LOCUS       DASPGK010000001         7551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_351298, whole genome shotgun sequence.
ACCESSION   DASPGK010000001 DASPGK010000000
VERSION     DASPGK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351298"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /gene="priA"
                     /locus_tag="VEC56_00005"
     CDS             complement(<1..1393)
                     /gene="priA"
                     /locus_tag="VEC56_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXV12560.1"
                     /translation="MLPIPVDQPFTYQIPDSLIGRVNVGCRVEVPFGKRELSGVVVDV
                     METSDVARTRAIREVADAFAAPALLELANWIASYYGCSRGEAMQSVLPPSMRRAKLKA
                     RPTGTLRVVDETLVREGETGDAYAAVRRALSRAKRQFALLAELVSAGGEAAVPVILEE
                     WDYAREVIDGLVLRGIARIDPVDTVSSLDALEARVGELNREQTQTLALVVEALRAREF
                     RPILIHGVTGSGKTEIYLRAARHVVEAGGGCIVLVPEIGLLPQATARYRRAFGDLVAI
                     IHSRLTGPERFEIWERIERGEHRVVVGPRSAVFSPVRDLRLIVVDEEQDDSYKQDDKP
                     RYHARAVALVRGKMEKAAVVLGSATPSAESMRWAREGKYASARLFERVAGGVLPEIEI
                     VDMREPDARGAVLSPKLSEHIERTVGAGRQAIVFLNKRGHARTVQCSACGWVARCKNC
                     DVSLTFHRVDNRLK"
     gene            complement(1520..1897)
                     /locus_tag="VEC56_00010"
     CDS             complement(1520..1897)
                     /locus_tag="VEC56_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV12561.1"
                     /translation="MALGKILVVDDEVYILHILDFSLGAEGFEVITANNGELAIEKAK
                     QEKPNLVVLDVMMPVLDGYETCRRLKRDPETKNIPVVLLTAKGRDLDKRLGYEVGAVD
                     YIVKPFSPSRLIERIQEILGCRK"
     gene            complement(1899..5225)
                     /locus_tag="VEC56_00015"
     CDS             complement(1899..5225)
                     /locus_tag="VEC56_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV12562.1"
                     /translation="MNERVQMAHLTGTPTEYLVVAGVLVGLVFLTLWRETIATNSALF
                     FSRTMAALAFAMSRVTVVVFAASMIEEPSGAPFLYILLNFACMYLAARCLPVGIQEPR
                     GSAGETPPGEDGTEAPSPRGLADADWRAVKYQTAVFGLTAGLAYVVIPQASTGFSILG
                     FLGAILLGATVRVCGALFSASHNAIAEVRPVLFLLVASLVTSWLLVVAAGTTSGATAA
                     MLIKAAQLADIGVLVTGLLSLQTSYIVIGTRMRREAEEKALEIGQARAELTLLNRVAS
                     DMYEDSHSMIRRQQDHSRSLIRRAENLERILEIGVRIQQQKNVKELLQMVAELIRENL
                     GFETVVVRVLNKRTQAFETKAYVGLNPEAQDAVMNHRISVVDYEQMIDPRSRVSRSFF
                     LKNPGPIVSGADAPARDGGAELVSDHWEDVERLIVPLIDDDAGEQTIGYVSVEEPGDP
                     QRSVVEVIETLETIATLAAVAIRNLNRHREVGEKNEKLKQYAEKLSSLNKMKSNFVAT
                     ISHEFRTPLTSIKAYCETLNKNAESIDRTILKEFLKVIEEESNRLMSLIEDILDFSQM
                     ESGAVRFERTSCNLNEIVTVAANELEKNFESKRITLEARLPATEVKVRAERDLMKQLA
                     INLLHNASKFTPEGGKVWIRLEDEVVSARIIVEDNGIGIPEDQLEKIFDHFHQVDGTA
                     TRRHGGTGIGLAICKNIVEWHDGRIWVENVPGKGARFVVVIPKKQAVVRSELLTDYVS
                     TRRFEVERYMELVVELVAELLNVKRASLMMVDDDGKELRIDTAIGLDEDVVEHARVKL
                     GEAISGTVASENNAFLVRDVDRERSMQPGGPSRNYESKSFLSVPLRKDGRVLGVINVT
                     DPVGRDHLDEADHALLELFADRVVLAMDALSHFKEKSREFEDVRSTFKSILDAKRYID
                     TQNVDALSGVLKDVADTLGMSPAESATLRYVFNVYDLGLAKVGHNIIKQPRELTHQDR
                     FDVEQHTIVGTDMLKPIEFIPRVRDAVLYHHENYDGSGYPGKLSGDEIPLDARIIRVA
                     DTFRALISHRPYQKQYSLSEAVQVLKHRAGTLFDPKVVDAFVEAVMRHADSFRTPGAS
                     VDSRRELDRSMLQQSLFKED"
     gene            complement(5244..>7551)
                     /locus_tag="VEC56_00020"
     CDS             complement(5244..>7551)
                     /locus_tag="VEC56_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV12563.1"
                     /translation="AISLETLLKIVAKEATLLLERPWAAIVLFNQKQEVTHSVFVGVN
                     QNRELKLEKKMRRGGLSEWIWTHNTPIVVEDATRDKRANCSEFLGHFGIRSIVGFPLT
                     SGKQVMGIVFTGDFAARTMSDTHMRLLTTLCQQLAVAIEKSRLYDSLERKIRELERQL
                     DTLEKTNILKSDFVSHVSHELRTPLTAIKAYVEALVDNSEDPKFLQRKEFLDIVSKET
                     NRLIRIVNDVLDVSKIEFGQRPMTRTPVDLPAVVADVLDMLSPTLRERSVRVEADVPS
                     DLPRIDGDADMIKQVFINLISNAIKYSPRGTTVTVAAREEAVNILVSVADQGVGIPKD
                     EIGNVFDKYFRARSQKESKMEGLGLGLAIVKNIVEQHGGAVRVESHEGKGSTFYFTLP
                     REHCFNDLIGYIGEVVDAREQLHEMLELIVRMIAELLNAKRVSLMLLDKSRSELFIKM
                     SFGIEEWVVENTRVRVGQGIAGMVVERGEPLLIANIEENDIYIAPNSPQYDTVSLVSV
                     PLRVNDVVVGVINVNNKTNGRPFDRDDLNLLMSFSERISRALERVRVVDDSHTFLQDT
                     IDAFKRMLETQTKTKVIEQAIDHAVKIARKLRLPEKDVSVIQYVASVHDIGMTEISDE
                     ILNKALHLSTEEMRQIQRHPERGAELIRPLEFVEAVSNIILYHHERFDGSGYPMGLKG
                     EEIPVGARILAVIDAYQSMTIGRPYRSRKSTEDALDELVDNSGTQFDRDVVEAFAGVL
                     KEDGKLAARDERELVRRLRGVPVGASQD"
BASE COUNT         1229 a         2474 c         2395 g         1453 t
ORIGIN      
        1 acttgagacg attatcgacg cggtggaagg tgagtgacac gtcgcagttc ttgcatcgcg
       61 ccacccaacc gcacgccgag cactgcacgg tgcgcgcgtg gccgcgtttg ttgaggaaca
      121 cgatggcctg gcggcctgcg ccgacggtgc gctcgatgtg ctcggagagc ttgggcgaga
      181 gcaccgcacc gcgcgcgtcg ggctcgcgca tgtcgacgat ttcgatttcg gggagcacgc
      241 ccccggcgac gcgttcgaag aggcgagcgc tggcgtactt tccttcgcgc gcccagcgca
      301 tcgactccgc cgacggtgtg gccgacccca gcacgaccgc cgccttctcc attttgccgc
      361 gcaccagcgc caccgcgcgc gcgtggtagc gcggcttgtc gtcttgtttg tacgagtcgt
      421 cctgctcttc gtcgaccacg atcaaacgca ggtcgcgcac cgggctgaac accgcggagc
      481 gcggcccgac cacgacacgg tgctcgccgc gctcgatgcg ttcccagatc tcgaagcgct
      541 cgggtcccgt gagccggctg tgaatgatcg cgaccaaatc gccgaacgcg cgccggtagc
      601 gcgcggtggc ctgcggcaaa agcccgatct cgggcaccag cacgatgcac ccgccgcccg
      661 cttcgaccac gtgacgagcc gcgcgcaggt agatttccgt cttgccgctg ccggtgacac
      721 cgtggatcag aatgggacgg aactcgcgtg cgcgcagcgc ctcgaccacg agtgcgagtg
      781 tctgcgtctg ctcgcggttc agttcgccga cgcgcgcttc gagcgcgtcc agcgacgaga
      841 ccgtgtccac cggatcgatg cgtgcgattc cacgcaaaac caggccgtcg atcacctcgc
      901 gcgcgtagtc ccactcctcc aggatcacgg gtaccgccgc ctcgccgccc gcgctcacca
      961 gctcggcgag gagcgcgaac tgccttttcg cgcgcgatag cgcgcgtcgc actgcggcgt
     1021 aggcgtcgcc cgtctcacct tcccgtacca gcgtctcgtc gactacacgg agcgtaccgg
     1081 tcggtcttgc cttcaacttc gcgcggcgca tgctgggagg aagcaccgac tgcatcgctt
     1141 cgccgcgcga gcacccgtag tatgacgcga tccagttcgc cagctcgagg agcgcgggtg
     1201 ccgcgaacgc gtccgcgacc tcgcggatcg cgcgcgtgcg agcgacgtcg ctcgtctcca
     1261 tgacatcgac tacgacaccg ctcaactcgc gtttgccgaa cggcacctcg acgcgacacc
     1321 cgacgttgac ccgtccgatc agactatccg gaatttgata cgtgaacggc tggtcgacgg
     1381 ggatcggaag cacgacgcgc gcgaagcggg gcgtgtcaga cggtagtagc gtgggatcgg
     1441 gccgcatgcg cggcagtata gcacctggaa cgaatgctgc cggggggctc accgtatggc
     1501 gagcgtcccc cggcgtggcc tacttccggc agcccaagat ctcctgaatg cgctcgatca
     1561 aacggctcgg gctgaacggc ttgacgatgt agtcgacggc cccgacttcg tagcccaaac
     1621 gcttgtcgag gtcgcggccc ttcgcggtca atagcacgac cgggatgttc ttggtctccg
     1681 ggtcgcgttt caagcggcgg cacgtttcgt acccgtcgag gaccggcatc atgacgtcca
     1741 gcacgacgag gttcggcttc tcctgcttgg ccttctcgat cgccagctcg ccgttgttcg
     1801 cggtgatcac ttcgaatccc tccgcgccca ggctgaagtc gagaatgtgc agaatgtaaa
     1861 cctcgtcgtc gacgaccaga atcttgccca gcgccatgtc agtcctcctt aaatagactc
     1921 tgctgcagca tcgaccgatc gagctcccgt cgcgaatcga cggaagcgcc cggtgtgcgg
     1981 aagctatcgg cgtgtcgcat gaccgcttcc acgaacgcgt cgaccacctt ggggtcgaac
     2041 agcgtcccgg cgcgatgctt tagaacctga actgcctcgc tcaagctgta ctgcttctga
     2101 tacggacggt gcgaaatcag ggcgcggaac gtatccgcga ctctaatgat gcgcgcgtcc
     2161 agcggaatct cgtctcccga cagcttgccg gggtatccgg agccgtcgta gttctcgtgg
     2221 tggtagagca cggcgtcgcg cacgcgcgga atgaactcga tcggcttgag catgtcggta
     2281 ccgacgatcg tgtgctgctc cacgtcgaag cggtcctggt gcgtcagctc gcgcggctgc
     2341 ttgatgatgt tgtgccctac cttggccaat cccaagtcgt aaacgttgaa cacgtagcgc
     2401 aacgtggcgc tctctgccgg gctcatgccc agtgtgtcgg cgacgtcctt cagcacgccc
     2461 gagagcgcgt cgacgttctg ggtgtcgatg tagcgcttgg cgtccaggat cgacttgaac
     2521 gtcgagcgta cgtcctcgaa ctcgcgcgac ttctccttga agtgcgacag cgcgtccatg
     2581 gccaggacga cgcggtccgc gaacagctcg agcagcgcgt ggtcggcttc gtcgaggtga
     2641 tcgcggccga ccgggtcggt aacattgatg acgcccagca cgcgcccgtc cttgcgcagc
     2701 ggcaccgaca gaaacgactt cgactcgtag tttcggctcg gtccgcccgg ctgcatgctg
     2761 cgctcgcggt ccacgtcccg caccaggaag gcgttgttct cgctcgccac cgtgcccgag
     2821 atcgcttccc cgagcttgac gcgcgcgtgc tcgaccacgt cttcgtcgag gccgatggcg
     2881 gtgtcgatgc ggagctcctt gccgtcgtcg tccaccatca ttagagaggc gcgcttgacg
     2941 ttgagcagct ccgcgaccag ctcgaccacg agttccatgt agcgctccac ctcgaagcgc
     3001 cgcgtcgata cgtagtcggt cagcaattcg ctgcgcacga ccgcctgctt cttggggatc
     3061 acgaccacga agcgggcgcc cttgccgggg acgttttcga cccatatgcg accatcgtgc
     3121 cattccacga tgttcttgca gatcgccaac ccgatgccgg tgccgccgtg gcggcgcgtg
     3181 gcggtcccgt ccacctggtg gaagtggtcg aagatcttct ccagctggtc ctccgggatg
     3241 ccgatcccgt tgtcctccac gatgatgcgg gcgctcacga cctcgtcttc caggcgaatc
     3301 cacaccttgc cgccctcggg ggtgaacttg gacgcgttgt gcagcaggtt gatggcaagc
     3361 tgcttcatca ggtcgcgttc ggcacgcacc ttgacctcgg tggccggcag ccgggcttcc
     3421 aaggtgatgc gcttgctctc gaagttcttc tccagctcgt tggccgccac cgtcacgatc
     3481 tcgttcaggt tgcaggacgt gcgctcgaag cgaaccgccc cggactccat ctgcgagaag
     3541 tcgaggatgt cctcgatcaa cgacatcagg cggttgctct cctcttcgat gactttcaag
     3601 aactccttca agatcgtgcg gtcgatcgac tccgcgttct tgttgagcgt ctcgcagtac
     3661 gctttgatcg acgtcagcgg agtgcggaac tcgtgcgaga tggtcgcgac gaaattcgat
     3721 ttcatcttgt tgaggctcga cagtttttcc gcgtactgct tgagcttctc gttcttctcg
     3781 ccgacctcgc ggtggcggtt caggttgcga atggcgaccg ccgccagggt cgcgatggtt
     3841 tccagggttt cgatcacttc caccacggag cgctggggat cgccgggctc ttcgaccgag
     3901 acgtacccga tggtctgctc gccggcgtcg tcgtcgatga gcggcacgat cagccgctcg
     3961 acgtcctccc agtggtcgct gaccagctcg gcgccgccgt cgcgcgcggg ggcatccgcg
     4021 ccgctgacga tcggccccgg attcttgagg aagaaactgc ggctcacgcg cgagcgtggg
     4081 tcgatcatct gctcgtagtc gacgaccgag atgcggtggt tcatcaccgc gtcctgcgcc
     4141 tcggggttga gccccacgta ggccttggtc tcgaaggctt gcgtgcgctt gttgagtacg
     4201 cgcacgacaa ctgtctcgaa gcccaggttt tcgcgaatca gctcggccac catttgcagc
     4261 agctccttga cgttcttttg ctgctggata cgaacaccga tctcgagaat gcgctcgagg
     4321 ttctccgcgc ggcgaatgag cgagcgcgag tgatcctgct ggcgccggat catggagtgc
     4381 gagtcctcgt acatgtccga cgcgacgcgg ttgaggagcg tgagctcggc gcgcgcctgg
     4441 ccaatctcga gcgccttttc ctcggcctcg cggcgcatgc gcgtaccgat cacgatgtag
     4501 ctcgtctgca gggagagcag gcccgtgacg agcactccga tgtcggcgag ctgcgccgcc
     4561 ttgatgagca tggccgcggt cgcgcccgac gtcgtgccgg cggctacgac caggagccac
     4621 gacgtcacca acgacgccac caataggaat agcaccgggc gcacttcggc gatcgcgttg
     4681 tgcgacgcgc tgaacagcgc gccgcacacg cgcacggtgg cgcccaagag aatcgcgccg
     4741 aggaagccga gtatggaaaa gccggtgctg gcctggggaa tcacgacgta ggcgaggccc
     4801 gcggtcagcc cgaatacggc cgtctgatac ttgacggcgc gccagtccgc gtcggcgaga
     4861 ccgcgcggcg agggcgcctc ggtgccgtcc tcgcccggcg gcgtttcacc cgcggagccg
     4921 cgcggctcct ggatgccgac cggaagacag cgcgcggcca gatacatgca cgcgaaattg
     4981 agcagaatat agaggaacgg cgcgcccgac ggctcttcga tcatggaggc ggcgaatacg
     5041 accacggtga cgcggctcat cgcgaacgcg agcgccgcca tcgtgcgcga gaagaacagt
     5101 gccgagttgg tcgcgatcgt ctcccgccac aaggtcagga acacgaggcc gaccaatacg
     5161 cccgccacga ccaagtactc ggtcggggtg ccggtcaaat gcgccatctg cacacgttcg
     5221 ttcatggtcc ccgtgccgct ccgtcaatcc tgcgacgcgc cgacgggtac gccgcgcagt
     5281 cgccgcacca gctcgcgctc gtcgcgcgcg gccagctttc cgtcttcctt gagcacgccg
     5341 gcgaacgcct ctaccacgtc gcggtcgaac tgggttcccg agttgtcgac cagttcgtcg
     5401 agcgcgtcct cggtcgattt gcgcgaccgg tacggccgtc cgatcgtcat cgactgatag
     5461 gcgtcgatca cggccaggat gcgcgcgccc accggaatct cctcgccctt gaggcccatg
     5521 ggataaccgc ttccgtcgaa gcgttcgtgg tgatacagaa tgatgttgct caccgcttcc
     5581 acgaactcga ggggacgaat cagctcggcg ccgcgctcgg ggtggcgttg gatctgccgc
     5641 atttcttcgg tcgacaggtg cagcgccttg ttcaagatct cgtcgctgat ctcggtcatg
     5701 ccgatgtcgt gcacgctggc gacgtactgg atgacgctga cgtccttctc gggcagccgc
     5761 agcttgcgcg cgatcttgac cgcgtggtcg atggcctgct cgatcacctt ggtcttggtc
     5821 tgcgtctcca gcatgcgctt gaacgcgtcg atggtgtcct gcaggaacgt gtgcgagtcg
     5881 tccaccacgc gcacgcgctc cagcgcgcgc gaaatgcgct cgctgaacga catcagcaga
     5941 ttcaagtcgt cgcggtcgaa ggggcgtccg ttggtcttgt tgttgacgtt gatcacgccc
     6001 acgacgacgt cgttgacccg cagtggtacg ctcaccagcg acacggtgtc gtactgcggg
     6061 ctgttgggag cgatgtagat gtcgttctcc tctatattgg cgatcagcag cggctctccg
     6121 cgctccacca ccatgccggc gatgccctgg cccacccgta cccgcgtgtt ctcgaccacc
     6181 cactcctcga ttccgaacga catcttgatg aagagctcgg agcgactctt gtcgagcagc
     6241 atcagcgaga cgcgcttcgc gttcaggagc tcggcgatca tgcgcacgat cagctcgagc
     6301 atctcgtgga gctgctcgcg cgcgtccacc acttcgccga tgtaaccgat gagatcgttg
     6361 aagcagtgct cgcgcggaag cgtgaagtag aaggtgctgc ccttgccctc gtggctctcg
     6421 acgcgcacgg cgccgccgtg ctgctcgacg atgttcttga cgatcgcgag ccccagcccg
     6481 aggccctcca tcttcgattc cttctgcgag cgcgcccgga agtacttgtc gaacacgttg
     6541 ccgatctcgt ccttgggaat gcccacgccc tggtcggcca ccgagaccag gatgttgacg
     6601 gcctcctcgc gggccgcgac ggtgaccgtg gttccgcgcg gcgagtactt gatcgcgttc
     6661 gagatcaggt tgatgaagac ctgcttgatc atgtcggcgt cgccgtcgat gcgcggcagg
     6721 tcgctgggga cgtcggcttc gacgcgcacg ctgcgctcgc gcagcgtcgg gctcagcatg
     6781 tcgagcacat cggcgaccac cgccggcagg tcgaccggcg tgcgcgtcat cggtcgctgg
     6841 ccgaactcga tcttcgagac gtcgagcacg tcgttgacaa tgcgaatgag gcggttggtc
     6901 tccttggaca cgatgtccag gaattccttg cgctggagga acttcggatc ctcggagttg
     6961 tcgaccaggg cctcgacgta ggccttgatg gcggtcagcg gcgtgcgcag ctcgtgtgac
     7021 acgtgcgaca cgaaatcgga cttcaagatg ttggtctttt ccagcgtgtc gagctggcgc
     7081 tcgagctcgc ggatcttgcg ctccagggag tcgtataggc ggctcttttc gatcgcgacg
     7141 gcaagctgct ggcacagggt cgtcaagagg cgcatgtggg tgtccgacat cgtgcgcgcc
     7201 gcgaagtcgc cggtgaagac gatgcccatt acctgcttgc cgctcgtgag cgggaagccc
     7261 acgatcgacc gtatgccgaa gtggccgagg aattcgctgc agttcgcgcg cttgtcgcgc
     7321 gtcgcatcct cgaccacgat gggcgtgttg tgggtccaga tccactcgct caagccgccg
     7381 cgccgcatct tcttttcgag cttgagttcg cggttctggt tcacgcccac gaagaccgag
     7441 tgcgtcactt cctgtttctg attgaagagc acgatcgcgg cccacgggcg ctcgagcagc
     7501 agggtcgctt ccttggccac gatcttgagg agcgtctcca gcgagatggc g
//
LOCUS       DASPGK010000002         6350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_159, whole genome shotgun sequence.
ACCESSION   DASPGK010000002 DASPGK010000000
VERSION     DASPGK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2095
                     /locus_tag="VEC56_00025"
     CDS             <1..2095
                     /locus_tag="VEC56_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXV12564.1"
                     /translation="DFVAVHQFGFLDRYDTLAAARDGATLLLNSPHPAETVWEHIPER
                     VQRQIVEKKLVVHCVDAYRLAREHEMGVRINAIMQTAFFALSGVLPRDEAIAHIKRYI
                     QKTYGKRGEAVVRANHAMVDATLSHLHQVTVRKGARAPASARATTGDFVARLLAGEGD
                     ALPVSAFPVDGTYPTGTTRVEKRNIAQEVPVWETDLCIQCGKCVLVCPHSVIRAKVVG
                     VDATSGAPQGFAFEDARWRELEGMKYTLQVSVDDCTGCRLCVEICPARDKSNVSRKAI
                     NMAPRASIRADHRAHWDFFSALPDTFVPDAGEALGLKYNKLKDVQMIPPLFEFSSACA
                     GCGETPYLRLMSQLFGDRLLIANATGCSSIYGGNLPTTPWSTDRDGRGPAWSNSLFED
                     NAEFGLGMREAIDAQAALARRLVEANRVAIGADLADTILSADQSTESSVSAQRHRVAE
                     LKARIRDRLGAQAWAADLLAVADTLVRKSVWIVGGDGWAYDIGFGGLDHVLASGRNVK
                     LLILDTEVYSNTGGQSSKATPLGAVAKFASGGKATAKKDIGAIARSYGHVYVAQIAMG
                     AGDQQTLRAFVEAESYHGPALIIAYSHCIAHGIDMAKGMTQQKLASESGHWPLYRFDP
                     RRAAQGENPMQLDSAPPKIPLKDYIYNEGRYRMLLQSDPDAAAMLLAAAQAHVNEQWK
                     HLQREAWTPNPEEKK"
     gene            2092..3108
                     /locus_tag="VEC56_00030"
     CDS             2092..3108
                     /locus_tag="VEC56_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HXV12565.1"
                     /translation="MSDLRTKYLGLELKNPLVHSASPLSESLDKMKMLEDAGCSAIVM
                     HSLFEEQIQHESRQLDHYLSYGSQSYAEALSYFPEAQSYRVGPDEYLNRIANAKRALS
                     IPVIGSLNGVSRGGWVEHARLIQEAGADALELNIYMIPTDVEMSGPEVEQMYIDTLSA
                     VRAEVTIPLAVKVGPYFSAMGHMAKRLRAAGADGLVLFNRFYQPDFDLETLEVVPHLV
                     LSTSWEMRLPLRWVAILFGRVDVDFAITTGVHTPEDVIKGVMAGARVTMMTSAILQHG
                     MGRVKVVLDGVERWMRENEYVSIGQMCGSMSQKNVAEPAAFERANYMRMLHSWQSDPT
                     GFLP"
     gene            3295..3672
                     /locus_tag="VEC56_00035"
     CDS             3295..3672
                     /locus_tag="VEC56_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV12566.1"
                     /translation="MIIDGGLPTVFISDMDRSIAFYTGVLELKLRERSGNYWALIETR
                     EGLIFGLHPASSHGPPSGTVGSITIGFNVTRPLDEVVDKLRARGVRFHASIKEGAEAG
                     LRTVFFGDPDGNQFYLCEATEER"
     gene            3881..4402
                     /locus_tag="VEC56_00040"
     CDS             3881..4402
                     /locus_tag="VEC56_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12567.1"
                     /translation="MVLSEFQDCYIVDDAPRLHTLTLMHRFNTGSIAARFKLEANAGM
                     AMTYVSETHAWPSLGNMRTGISVCYGSCQAGDTVLGTVTYQGYGMSHSCAQFIVVPHP
                     DAEALDVVTCGNEPELVTTFHFEVFEGPGGGCSLCINHDPGIQYGGDPELFDCQPVSS
                     NSSTWGAIKALYR"
     gene            4416..4994
                     /locus_tag="VEC56_00045"
     CDS             4416..4994
                     /locus_tag="VEC56_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12568.1"
                     /translation="MMIHSGGRTNRRGKQFILLGLSALVTAAEVGAQESASIVPGERI
                     RLRSSAASSWIVGNYLGTEGDRVRVQTEAAAPHLASTSSISELEVSRGSKSHAGRGAI
                     IGMTVGAVGGIVAIVMDDQDNADGSSNSFNGMDALAVPLFAALGAGVGALVGGMMRAE
                     QWVPMSKTSLSIGPASRTMPIGVALTIPMGGR"
     gene            5042..>6350
                     /locus_tag="VEC56_00050"
     CDS             5042..>6350
                     /locus_tag="VEC56_00050"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12569.1"
                     /translation="MRRRAPIAVVALAVLLNLANTPAQERRSTLNPKPPSSADARELS
                     ARIDTLLIARQADSVQAIVARSLPDAFAEGDSALVSALLLARAQANQMGGRMREAREA
                     FAAVREWAEGSRDSARWMDALGGGAYSAGVLGMRDESVALNTRLLEIALATDDLIREA
                     RARTGRAYVAMVGGDLASARAGYARSIRLYRMAGDGRAELVPLVGLGRVYSSLGQIDS
                     ARACFKRVWLSATEMGQQVEAAHALNNLGTLEHRYGDLMLAMQYYQTAFEIHRAQGET
                     MGAMTAASNLALAHQACGRYDKAAEILQEQLAVCEDAGTRPQAGLVLKNLGFAYASAR
                     RIQSANATFRRAAAMGDTLDVQVRIDALWGLADALAVDSLDAAIEVLRTGIETIGQGD
                     LTLDLKSRLTLARLLLDRQDGEDAIIEIRRALSASEGLPPDRMG"
BASE COUNT         1128 a         2065 c         2100 g         1057 t
ORIGIN      
        1 ggacttcgtc gccgtgcacc agttcggctt tctcgatcgc tacgacacgc tggcggcggc
       61 acgcgacggc gcgaccttgc tcctcaacag cccgcatccc gcggagacgg tgtgggagca
      121 cattcccgag cgcgtccagc ggcagatcgt agagaagaaa ctggtcgtcc attgcgtcga
      181 cgcataccgg ctggcacgcg agcacgagat gggcgttcgc atcaacgcca tcatgcagac
      241 ggcgttcttc gcgctctcgg gagttcttcc acgcgacgag gccatcgcgc acatcaagcg
      301 ctacattcag aagacctacg gcaagcgcgg ggaagcggtg gtgcgcgcca accacgctat
      361 ggtggacgca acgctgtcgc acctgcacca ggtgacggtg cgcaaaggcg cgcgtgcacc
      421 cgcatccgcg cgcgcgacca cgggcgattt cgtcgcgcga ctcctggccg gcgaaggcga
      481 cgcgctgccg gtctccgcgt tcccggtcga cggaacctat cccaccggga cgacgcgcgt
      541 cgagaagcgc aacatcgcgc aggaagtccc ggtgtgggag accgacctgt gcatccagtg
      601 cgggaagtgc gtgttggtgt gcccgcactc ggtgatccgc gccaaggtgg tgggcgtcga
      661 cgcgaccagc ggcgcgcccc aaggcttcgc cttcgaggac gcgcgctggc gcgagctcga
      721 gggcatgaaa tacacgctcc aggtctcggt ggacgattgc acgggctgcc ggctgtgcgt
      781 ggagatttgc cccgcgcgcg acaagagcaa cgtctcgcgc aaggcgatca acatggcgcc
      841 gcgcgcctcg atccgcgcgg accaccgcgc gcactgggac ttcttttcgg ccctgccgga
      901 caccttcgtg cccgacgcgg gcgaagcgct gggcttgaag tacaacaagc tcaaagacgt
      961 gcagatgatc ccgccgctct tcgagttctc gagcgcgtgc gcggggtgcg gcgaaacgcc
     1021 gtacctgcgc ctgatgagcc agctcttcgg cgatcgcctg ttgatcgcga acgccaccgg
     1081 ctgctcgtcg atctacggcg gcaatctgcc caccaccccg tggtcgaccg atcgcgacgg
     1141 ccgcggtccc gcgtggagca actcgctctt cgaagacaac gcggagttcg ggctcggcat
     1201 gcgcgaggcg atcgacgcgc aagcggcgct ggcgcgccgt ctggtcgaag ccaatcgcgt
     1261 cgcgatcggc gccgatctcg cggacacaat cctctccgcg gaccagagca cggagtcgtc
     1321 ggtgtctgcg cagcgccatc gcgtggccga gttgaaggcg cgcatccgcg atcgcttggg
     1381 cgcgcaggcg tgggcagccg atctcctcgc cgtggcggac accttggtgc gcaagagcgt
     1441 gtggatcgtg ggcggcgacg ggtgggcgta cgacatcggc tttggcggcc tcgaccacgt
     1501 gctcgccagc gggcgcaacg tgaagctcct gatcctggac acggaggtct actccaacac
     1561 cggcgggcag tcgtcgaagg cgaccccgct gggtgcggtg gcgaagttcg cgagcggcgg
     1621 caaggcgacg gccaagaaag acatcggcgc catcgcacgc tcgtacggcc acgtctacgt
     1681 ggcgcagatc gcgatgggcg cgggcgacca gcagaccttg cgcgccttcg tcgaagcgga
     1741 gagctaccac ggtcccgcgc tcatcatcgc ctacagccac tgcatcgcgc acgggatcga
     1801 catggcgaag gggatgacgc agcagaagct cgcgtccgag agcggccact ggccgctcta
     1861 ccgcttcgac ccgcgccggg ccgcgcaggg cgaaaacccc atgcagctcg acagcgcgcc
     1921 cccgaagatc ccgctcaagg actacatcta caacgagggc cgctaccgca tgctgctcca
     1981 gtccgacccg gacgcggcgg cgatgctgct cgccgccgcc caagcccacg tcaacgagca
     2041 gtggaagcac ctccagcgcg aagcctggac gcccaacccg gaggagaaga agtgagcgac
     2101 ctacgcacga agtacctggg cctcgaactc aagaatccgc tcgtgcactc ggcgtcaccg
     2161 ctgtccgaat cgctcgacaa gatgaagatg ctcgaggacg cgggctgctc ggccatcgtg
     2221 atgcactcgt tgttcgagga gcagatccag cacgagagcc gtcagctcga tcactacttg
     2281 agctacggtt cccagagcta cgcggaggcg ctctcgtatt ttcccgaggc gcagtcgtac
     2341 cgcgtggggc ccgacgaata tctcaatcgc atcgcgaacg ccaagcgcgc gctttccatt
     2401 ccggtcatcg ggagcttgaa cggggtgtcg cgcggcggct gggtggagca cgcccgtttg
     2461 atccaggaag cgggcgcgga cgctctcgag ctcaacatct acatgatccc gaccgacgtc
     2521 gagatgtccg gccccgaggt cgagcagatg tacatcgaca cattgagcgc ggtgcgcgcc
     2581 gaagtgacca ttccgctcgc ggtcaaggtg gggccctatt tctccgccat gggccatatg
     2641 gcgaagcggc tccgggctgc cggcgccgac ggactggtgc tgttcaatcg cttctaccaa
     2701 cccgacttcg acctggaaac gctcgaagtc gtgccgcacc tggtattgag cacgtcgtgg
     2761 gagatgcggc tgccgctgcg ctgggtcgcg atcctcttcg gccgcgtcga cgtcgacttc
     2821 gcgatcacga cgggcgtgca cacgcccgag gacgtcatca agggtgtgat ggcgggcgcg
     2881 cgcgtcacca tgatgacgtc ggccatcctg cagcacggca tgggtcgcgt gaaagtcgtg
     2941 ctcgacgggg tggagcgctg gatgcgggag aacgaatacg tttcgatcgg gcagatgtgc
     3001 ggcagcatga gccagaagaa cgtggccgaa ccggccgcct tcgaacgcgc gaactacatg
     3061 aggatgctgc actcgtggca gtcggacccg acgggatttc taccctgacg gttgtccgta
     3121 gtcctggcga actcctcgcc cgaagcgtat actctggacg aggccccgcc ggacagaatt
     3181 cgcgaatcga aggtcccacc ttaaccgcca cggcaattgt cccgcctgta cctccgccac
     3241 gtgctgctct ctccttcgtc gtggtgagac tgaacgactc ctcaaggagg acccatgatc
     3301 atcgacggag gccttccgac cgttttcatt tccgacatgg accggtccat agcgttttac
     3361 accggcgtgc tggagctcaa gttgcgcgag cggtccggca actattgggc gctgatcgag
     3421 acgcgcgaag gactgatctt cgggctccat ccggcgtcgt cccacggccc gccttcgggg
     3481 acggtcggct cgatcacgat cggcttcaac gtgacgcggc cgcttgacga agtcgtcgac
     3541 aaacttcgcg cccgcggggt caggttccac gcgtccatca aggagggcgc agaggccgga
     3601 cttcgcacgg tgttcttcgg cgatcccgac ggaaaccagt tctatctgtg cgaggccacg
     3661 gaggagcgct agtacaacgt ccgccatccc agtcgaaggc ggacggctgt ccatcctgtg
     3721 ctataatcac caacagacgc ggtcctcata gtccgaagtg agtcccaacg atcggagcgt
     3781 catgatgcgc ccgcccatcc tccgcccgat cctttcgtcg cttgcggcgc tagctcttgt
     3841 cggcctggcc gccccccggc cggccctcgc ggacgcaatc gtggtcctgt ccgaattcca
     3901 agactgctat atcgtggacg acgcccctcg actgcacaca ctgactctca tgcatcggtt
     3961 caacaccggc tcgatcgcgg cccgcttcaa gctcgaggcc aacgcaggca tggcgatgac
     4021 gtacgtgtcc gaaacacacg cgtggccgag cctgggtaac atgcgcaccg ggatatcggt
     4081 ttgctatggg tcgtgccagg ccggcgacac ggtgctcggc accgttacat atcaaggcta
     4141 tggcatgtcg cattcatgcg cgcagttcat cgtggtgccg catcccgacg cggaggcgct
     4201 ggatgtcgtg acatgcggca acgagcccga gctcgtgacg acctttcact tcgaagtctt
     4261 cgagggtccg ggtggcggct gctccttatg cattaatcac gatcccggga tacaatacgg
     4321 cggagacccc gaattgttcg actgccaacc ggtgtcgagc aactcgtcca cgtggggagc
     4381 aatcaaggcg ctgtatcgat aaggcaggtt attcgatgat gatccatagt ggtggccgca
     4441 cgaatcgtcg cggtaaacag ttcatccttc tcgggttgtc tgcgctcgtc accgccgcgg
     4501 aagtcggcgc gcaagagtct gcgtcgatcg tgccgggaga acgaatccgc ctgcgctcga
     4561 gcgcggcaag ctcgtggatc gtcggaaact atctcgggac cgaaggcgac cgcgtgcggg
     4621 tccagaccga ggccgcggct ccccatctcg cgtcgacgtc atcgatttcg gaactcgagg
     4681 tgagccgggg aagcaagtcc cacgccggca ggggcgcgat catcggcatg acggtgggag
     4741 cggtcggggg catcgtcgcg atcgtcatgg acgaccagga caacgccgat gggagcagca
     4801 acagcttcaa tggcatggac gctctggccg taccgttgtt cgcagcgttg ggcgcgggcg
     4861 tgggcgcgct ggtaggaggc atgatgcgcg ccgagcaatg ggttcccatg tcgaaaacct
     4921 cattgagcat cggcccggcg tcgcgcacga tgccgatcgg cgtggcgctc acgattccga
     4981 tgggcgggcg ttgacgaccg cgctaaggtc tctcgggaga caccgtcgct cggagttcaa
     5041 cgtgagaaga cgcgccccca ttgctgtcgt tgccttggcg gttctgttga accttgccaa
     5101 cacaccggcc caggagcgcc ggtcgacact gaaccccaag cctccgtcga gtgccgacgc
     5161 gagggaattg tcggcccgca tcgacacact cctaatcgcc cgccaggccg attccgtcca
     5221 agccatcgtc gcccggagcc tccccgacgc ttttgcggag ggcgactccg cgttggtgtc
     5281 cgcactgctc ctggcgcgcg cgcaagccaa tcagatgggc ggtcgcatgc gcgaggcgcg
     5341 cgaggccttc gcggccgtgc gcgagtgggc cgaggggtcc cgcgactccg cgcgctggat
     5401 ggatgcgctt ggaggcggcg cgtactcggc cggcgttctg ggaatgcgcg acgagtcggt
     5461 agcgctcaat acccgcttgc tcgaaatcgc gctcgcgacg gacgacttga tccgcgaagc
     5521 acgggcgcgc acggggcgcg cgtacgtggc catggtcggc ggggacttgg cgagcgcgcg
     5581 cgccggctac gcacgctcga ttcgattgta tcgaatggcg ggcgacggcc gtgcggagtt
     5641 ggtgcccctc gtcggactcg ggcgcgtgta ttcgtccctg ggccagatcg actccgcgcg
     5701 tgcgtgcttc aagcgcgtct ggttgagcgc gaccgagatg gggcagcagg tcgaagcggc
     5761 gcacgcactc aacaacctgg ggacgctgga gcaccgctat ggcgacctca tgctcgccat
     5821 gcagtactac cagaccgcgt tcgagatcca tcgcgcgcag ggcgagacga tgggtgcgat
     5881 gaccgcggcg tcgaacctgg cgctggccca ccaagcatgc ggacgctatg acaaggccgc
     5941 cgagatcctc caagagcagc tcgcggtctg cgaagatgcg ggcacgcgac cacaagccgg
     6001 cctcgtgctg aagaacctcg gattcgcgta cgcgagtgcg cggcgaatcc aatcggcgaa
     6061 cgcgaccttc cgccgcgcgg cggcgatggg ggatacgctg gacgtgcagg tgcgcatcga
     6121 tgctctgtgg ggactcgccg acgcactcgc cgtcgacagc ctggatgcgg ccatcgaagt
     6181 cttgcgaacc gggatcgaaa caatcgggca gggcgacctt actctcgacc tcaagtcacg
     6241 gctcacactc gcacgactgc tgttggatcg tcaggatggc gaggacgcga tcatcgagat
     6301 tcggcgtgcg ctcagtgcga gcgagggact tcctcccgac cgcatgggcg
//
LOCUS       DASPGK010000003         7419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_468993, whole genome shotgun sequence.
ACCESSION   DASPGK010000003 DASPGK010000000
VERSION     DASPGK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468993"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2476
                     /locus_tag="VEC56_00055"
     CDS             <1..2476
                     /locus_tag="VEC56_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXV12570.1"
                     /translation="PRSIEYYQQAIALRETTGDLGGLGTSYTVLGHVYNRTGRYVQAR
                     DCYEKALPILEAQGNPQQMTEILSGIALLNVDMGRAADAEAAYRRAIEIAQRGELANA
                     EATLRRNLADNLRLQGRYGEALSNLEAASTLLDAHPDAIEQALVLSTRGATYLNMGEI
                     DDARTDLLKFSELAAQLENPSYVPLSQITIAEFYRETGAYERGLKPAQEAIAGAEKAG
                     DARLYREAVALRGDLEMRLGRYDAALASWQEALAQDTHDQAVRYVLEDEIAIASVYAS
                     MGRTEEARERLRDVAPRVRTAGLTNLESAILFAAGESFEREQADSAAFYYDAALARVE
                     SQGAGIGGAEVQSGYLSGIRRHYYEEVTRYYVSTFEATKDARWSKRAFATIEKAKARG
                     LLDMLHSAVAGQSSPEEVKLLDRLYSLDPAASDYTTMRAEIERRYVEARRARVSSALG
                     GLAGAEAAVDLDAIAGSLPDKTVLVEYALGDSVSFVWVVGRDGYDMARLPPRAQIETM
                     TRRVRDALSRVDGDPSHLLGASRAAYGALLAPVSARLAKAEAAVIVPDGVLFELPFEV
                     LLTAEPSNGGWNKQPFLARNLVTMYAPSAAVYASLKSTSGSGDYPRELLAVGNPDFSA
                     LATEQGAPLAQLPFADEEVGVLSAKIKNDRKLILTGKDAREATVKRELKNGAPRVVHL
                     ATHGLVDASEPERSSIVLSPGDGEDGYFRTLEIIATPTRSRLVVMSACESARGKVSRG
                     EGVVGLSRAFLASGAGSVVASLWAVSDESTAELMKAFYERMLGKKRPASRALNEARLA
                     LIETEKYSHPFYWSPFVVTGTERSPW"
     gene            2566..3117
                     /locus_tag="VEC56_00060"
     CDS             2566..3117
                     /locus_tag="VEC56_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12571.1"
                     /translation="MTRSLVHWAVALAVGAVAVDSFATQVIQRSPKELAQESQLVVDG
                     KVSSVRSYWNEDQSKIFTEAVVTVGSTYKGGAAQTVRVVQIGGVVGNVRMTAHGALQW
                     KKGEEVLLFLEPSVPGAFQVAGFSQGKYMIERDARTGKAFVKHALPPDMGEGKAPATD
                     GTSAKAAERVTLEQFIDDVLPNQ"
     gene            3172..>7419
                     /locus_tag="VEC56_00065"
     CDS             3172..>7419
                     /locus_tag="VEC56_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV12572.1"
                     /translation="MKTTLLKLSVLIAVVLLTAYAASGLIPDDADGDGVPDAEDACPA
                     EDASGFDRNGDGCIDDGSGARHVEYWGQTDATITYVINDQGAPGVSGNSDLEAIQDAF
                     DGWATIPGTELAVVYAGTTPQEIADGMDGVNLVTFVDTSYPFGLTTLAVGLTTSFETD
                     SLIDGRLYRPGEIFDADMIFNPSFAYGTSGASSVKDIHSIAAHEAGHLFGISHTAVRS
                     STMYFVLPGGLAARSLEDDDLAVYRKAYGSPATMSNSSRLEGTVTHGQSGDPMPGAIV
                     FVIDEATEDTTACDFTLPDGSFTFVGLPDGDYYVSIYPLDGTSNIDRMTPSNINALLV
                     ETTRTDFQAESYDAAESNTDDALDRTAVSVTEGSTTTVSIITNIDAEAPTVTSTNPAD
                     GTTGAPIDGAYVVRFSERLDFMTIASAFSFRDPMDVGVAGNIVLVHDDSVAVFTPSTP
                     LAFSTVYTLRFNTNLLDLAGNPLASEFALSIETEVEPPISVSSLAPNKGVVGNTVVIN
                     GRGFDPGATVMFGGQPAPVLREGPNSLLVRVPVGATTGDVAVTNPGPEVSNALVFTVL
                     SETEIARGYESGSVPLSDAPRAIALGPSGAFAYVAVAGGVDAVGADPSQSNYLSRTFI
                     DASGVFSDVATTPDGRRVYAVNETSGEITEIMSDPTVDPGLFHQVIASRPLGATPKGI
                     VVDPFGYRAYVSTDEAEIQVWDIRLGSPTYQQQVGVLYSPDGIGLAGEMAVPPAGDQL
                     LALASSGEVFFFELATGAVADRIAVDPDPRHLAIDPQGQRAYVADGNGNLSVLAIEGA
                     PFFVQDIATGGSLRGLDTTPAGFYLYATDRELDNLKIVDLDETHATFRSVIETTPQMS
                     NPVDVVISGDGIYAFSILQGDIAGGPRMAVTTIGIGPTIMSMFPTAGNPGTQVVFTMS
                     PLDEGIDSVEVDFNGILVPAQLQSLTMAIAIVPAGITSGPVTLLATNFGASSPETSNP
                     VQFTALEPSSPDNVRLSASLLPFCDYVTPAIAFSPSGDYLYTGCGGTNETNVYDVRPE
                     SPNFHTLIGEFAPAGGAGDIVWDIAITADGKVGLVAGGIQDPADPRRIQSFYADPNDP
                     RFLTRGPAVPTPDLQARIVSASPNNRTVLTFVEVDEIVVYDAAGIADGVVPTVVDSTS
                     TGGDVVDIAHHPSSRVAYVAVDTGVIRIFDTNPLSPAYGLGVGTVFTGFDDIWSLAVS
                     PDGARLYVYGWFNTDLADYRVLTYDASTPPLLTNPFSCTTNTETEVVQKQEFALAPQG
                     DFSVRTVEGVGYLRYNLVCPTDGFPTVSAPGSLMEFAFHPHGNRMYAAEQLSSRIGVY
                     DFVAAETLTEVSGNDQDGVAGETLPAPLRVRVTTSMPDRDMGGIPVRFAVATGGGSIV
                     TSGGPVTQAVVATDDDGFAQIFWKLGPALGPQTLGVSS"
BASE COUNT         1262 a         2507 c         2454 g         1196 t
ORIGIN      
        1 tccgcgctcg atcgagtact accagcaagc catcgcgttg cgcgagacga ccggcgactt
       61 gggcggactc ggcacgtcgt acacggtcct cggccacgta tacaaccgaa ccggacgcta
      121 cgtgcaggcg cgcgactgct acgaaaaagc gctcccgatt ctcgaagcgc agggcaaccc
      181 gcagcagatg accgagatct tgagcggcat cgcgctgctc aacgtcgaca tgggccgcgc
      241 cgcggacgcg gaggcggcct accgccgcgc gatcgagatc gcacagcgcg gcgagctggc
      301 caacgccgaa gcgacgctgc gccgcaacct cgccgacaac ctccgcctcc agggccgcta
      361 cggcgaggcg ctctccaact tggaggccgc gtcgacgctc ctcgacgcgc accccgacgc
      421 gatcgaacag gccctcgtgc tgtcgacgcg cggcgcgacc tacctgaaca tgggcgagat
      481 cgacgacgcc cgcaccgacc tcttgaagtt ctccgagctg gccgcccagc tcgagaatcc
      541 gtcgtacgtg ccgttgtccc agatcaccat cgccgagttc taccgcgaga ccggggccta
      601 cgagcgcggc ctcaagcccg cgcaggaagc gatcgccggc gccgagaagg cgggcgacgc
      661 gcgcttgtat cgcgaggccg tggccctgcg cggcgacttg gagatgcggc ttggccgcta
      721 cgacgccgcg ctggcgtcgt ggcaggaagc gctcgcgcag gacacgcacg accaggcggt
      781 ccggtacgtg ctcgaggacg agatcgcgat cgccagcgtg tacgcctcca tgggccggac
      841 cgaggaagcg cgcgagcgcc tgcgagacgt cgcgccgcgc gtgcgcacgg ccggtctgac
      901 gaatctcgag tcggcgattc tgttcgctgc cggcgagagc ttcgagcgcg agcaggccga
      961 cagcgccgcc ttctactacg acgcggcgct ggcccgcgtc gaatcgcagg gggccggaat
     1021 cggcggcgcc gaggtccagt cggggtatct ctcgggcata cgccgtcact actacgagga
     1081 agtgacgagg tactacgtct ccaccttcga agcgacgaag gacgcgcgct ggtcgaagcg
     1141 cgcctttgcg accattgaaa aggcgaaggc gcgcgggctc ctcgacatgc tgcattccgc
     1201 tgtcgccggt caatcctcgc ccgaagaagt gaagctgctc gaccgcctct actcgctgga
     1261 tcccgcggct tccgattaca cgacgatgcg tgccgagatc gagcgccgct acgtggaggc
     1321 gcgccgcgcg cgcgtgtcgt cggcgttggg cgggctggcc ggcgccgaag cggccgtcga
     1381 cctcgatgcg atcgccggct cgctgcccga caagacggtg ctggtcgagt acgcgctcgg
     1441 cgactcggtg tcgttcgtgt gggtggtcgg ccgcgacggc tatgacatgg cccggcttcc
     1501 gccccgcgcg cagatcgaaa ccatgacgcg gcgcgtccgc gacgcgctct ctcgcgtcga
     1561 cggggacccc agccatctgc tgggagcgtc gcgcgccgcc tacggcgccc tgctcgcgcc
     1621 cgtctccgcg cggctggcga aagcggaggc cgccgtgatc gtccccgacg gcgtgctctt
     1681 cgagctgccc ttcgaagtat tgttgacggc ggagccctcg aacggcggct ggaacaagca
     1741 accgttcctc gcgcgcaacc tcgtcacgat gtacgcgccc tcggccgccg tctacgcctc
     1801 tctcaagtcg acttcgggct ccggcgacta tccgcgcgaa ctgctcgcgg tcggcaatcc
     1861 cgatttttcg gcgctcgcca ccgagcaagg cgcgccgctc gcgcagcttc ccttcgccga
     1921 cgaagaagtg ggcgtgctgt ccgcgaagat caagaacgac cgcaagctga tcctcaccgg
     1981 aaaggacgcg cgcgaagcga ccgtcaagcg cgaactcaag aacggcgcgc cccgcgtcgt
     2041 ccacctcgcg acccacgggc tggtcgacgc ctcggagccg gagcgatcga gcatcgtgct
     2101 ctcacccggc gacggcgagg acggctactt ccgaacgctg gaaatcatcg ccaccccgac
     2161 gcgcagccgg ctagtcgtca tgtcggcgtg cgagtccgcg cgggggaaag tctcgcgggg
     2221 cgagggcgtg gtcggactga gccgtgcctt cctcgcgtcc ggtgcgggct ccgtcgttgc
     2281 gagcctatgg gccgtctccg acgagtcgac cgccgagctc atgaaagcat tctacgagcg
     2341 catgctggga aagaagcgcc ccgccagtcg ggcgctcaac gaagcgcgcc tcgcgctcat
     2401 cgagaccgaa aagtattcgc atccgttcta ctggtcgccg ttcgtggtga cggggacgga
     2461 gcgctcgccg tggtagtgga tccgcggtag ttcggggccc gcgcgggccc gtcctgcagt
     2521 tctcaatcaa tcggggagag gttcgcaaac cgagaggggt tcgtcatgac ccggagtctg
     2581 gttcattggg cggtggcgct ggctgttggc gccgtcgccg tggattcgtt cgcaacccag
     2641 gtgattcagc gctcgcccaa ggagctcgcg caggaatcac agttggtcgt cgacggaaag
     2701 gtctcgagcg ttcgttccta ctggaacgag gaccagtcga agatcttcac ggaggccgtg
     2761 gtgaccgtcg gttcgactta caagggcggc gccgcgcaga ccgttcgcgt cgtgcagatc
     2821 ggcggcgtgg tcggcaacgt gcgcatgacc gcgcacggcg cgctgcaatg gaagaagggc
     2881 gaagaagtcc tgctgttctt ggagccgtcg gtgcccggcg cgttccaggt cgccggcttc
     2941 tcgcagggca agtacatgat cgaacgcgac gcgcgcaccg gcaaggcgtt cgtcaagcac
     3001 gcgcttccgc ccgacatggg cgagggcaag gcgcccgcga ccgacggaac cagcgcaaag
     3061 gcagcggaac gggtcacgct cgagcagttt atcgacgacg tgctcccgaa ccaataggac
     3121 gatgcgcgac ggtgttgcat gcaacttgat cgtgcaacgg aagggctagc catgaagaca
     3181 acgctcctga aactctcggt gctcatcgcg gtggtcctgc ttacggccta cgccgcgtcc
     3241 gggttgatcc ccgacgacgc ggacggggac ggcgttcccg acgccgagga cgcgtgcccc
     3301 gccgaggatg cgtccggatt cgatcgcaac ggcgacggat gcatcgacga cggaagcggc
     3361 gcgcgacacg tcgagtattg gggccagacg gacgcgacca tcacctacgt gatcaacgac
     3421 cagggggcgc cgggcgtttc cggaaacagc gacctcgagg cgatccagga cgcgttcgac
     3481 ggctgggcga cgataccggg caccgagctg gcggtcgtct acgcgggcac gacgccccag
     3541 gaaatcgccg acgggatgga cggggtcaac ctcgtcacgt tcgtcgatac cagttatccc
     3601 ttcggcctga ccaccctggc cgtgggcctc accacgagct tcgagaccga ctcgctgatc
     3661 gacgggcggc tctatcgccc gggcgagatc ttcgacgccg acatgatctt caatccgtcg
     3721 ttcgcgtacg gaacgtcggg agcttcgtcg gtgaaggaca tccacagcat cgccgcgcac
     3781 gaagccggcc acctgttcgg catctcgcac accgcggtac gctcgtcgac catgtacttc
     3841 gtgctgccgg gcggtttggc ggcgcgctcg ttggaagacg acgacctcgc ggtgtatcgc
     3901 aaggcctacg ggagccccgc cacgatgtcg aactcgagcc gcttggaagg caccgtcacg
     3961 cacggtcaga gcggggaccc gatgccgggc gcgatcgtgt tcgtgatcga cgaagccacc
     4021 gaggacacca ccgcgtgcga cttcacactc ccggatggct cgttcacatt cgtcggcttg
     4081 cccgacggcg actactacgt atcgatctat ccgctggacg gcacctcgaa catcgaccgc
     4141 atgacgccca gcaatatcaa cgcattgctg gttgagacca cgcgcaccga cttccaggcc
     4201 gaatcctacg acgcggcgga atccaacacc gacgacgcgc tggaccgaac cgccgtgtcg
     4261 gtgaccgaag gaagcacgac gaccgtttcc atcatcacca acatcgacgc cgaagcgccg
     4321 acggtgacta gcacgaatcc tgccgacggg acaacgggcg cgcccatcga cggggcctac
     4381 gtggttagat tttcagagcg cctcgacttc atgacgatcg caagcgcatt ctcgtttcgc
     4441 gaccccatgg acgtcggcgt ggccggcaac atcgtgcttg ttcacgacga ctcggtggcc
     4501 gtcttcacgc cctcgacccc gctggcgttt tcgaccgtgt atacccttcg tttcaatacc
     4561 aacctcctgg atctggcggg caatccgctg gcgagcgaat tcgcgctcag cattgagacg
     4621 gaggtcgagc cgccgatcag cgtctcgagt ctcgcgccca acaagggcgt ggtgggaaac
     4681 accgtcgtga tcaacggccg cggcttcgat ccgggtgcca cggtgatgtt cggcgggcag
     4741 ccggccccgg tactgcgcga gggcccgaat agtctcctcg tgcgcgtacc cgtcggcgcc
     4801 acgacgggcg acgtcgcggt gaccaacccc ggtcccgaag tgagcaacgc gctcgtgttc
     4861 accgtgctct cggagacgga gatcgcgcgc ggctacgaga gcgggagcgt gccgctctcc
     4921 gacgcgccgc gggcaatcgc gctcggtccg agcggcgcat tcgcgtacgt ggcggtggcg
     4981 ggcggtgtgg atgcggtggg agccgatccc tcgcagtcga actacctctc gcgcacgttc
     5041 atcgacgcgt ccggagtttt ctccgacgtg gcgacgacac ccgacgggcg ccgcgtgtac
     5101 gcggtgaacg agacctcggg cgaaatcacg gaaatcatgt ccgacccgac cgtcgatccc
     5161 gggctgttcc atcaggtcat cgcgtcgcgt cccctgggcg cgaccccgaa gggcatcgtg
     5221 gtggatccgt tcggctaccg cgcctacgtc tcgaccgacg aggccgagat ccaggtgtgg
     5281 gacattcgtt tgggaagccc gacgtatcaa cagcaagtcg gcgtgctcta ctcacccgac
     5341 ggcatcggcc tggccggaga gatggcagtc ccgcccgccg gtgaccaact gctcgcgctc
     5401 gctagctccg gagaggtctt cttcttcgag ctcgcgactg gcgccgtcgc cgatcgcatc
     5461 gcggtcgacc cggacccgcg ccacctcgcg atcgacccgc agggccagcg cgcgtacgtc
     5521 gccgacggca atggcaatct ctccgtgctc gccatcgagg gggcgccgtt cttcgtgcag
     5581 gacatcgcga cgggcggatc gctgcgcggc ctggacacga cgccggccgg cttctacctg
     5641 tacgcgaccg atcgcgagct cgacaacctg aagatcgtcg acctcgacga gacccacgcg
     5701 acttttcgca gcgtcatcga gaccacgccg cagatgtcga acccggtcga cgtcgtaatt
     5761 tcgggcgacg gcatctacgc gttctcgatc ttgcagggcg acatagccgg cgggccacgc
     5821 atggcggtca cgaccatcgg catcggcccc accatcatga gcatgtttcc gaccgccggc
     5881 aatccaggga cgcaagtggt gttcacgatg agtcccttgg acgagggaat cgatagcgtc
     5941 gaagtcgact tcaacgggat cctggtgccg gcgcagctgc aaagcctgac tatggcgatc
     6001 gccatcgtgc ccgcggggat cacatccggc ccggtcacgc tgctcgcgac caacttcgga
     6061 gccagctctc cggagacgtc gaatcccgtg cagttcaccg cgctcgaacc gtcgagtccg
     6121 gacaacgttc gcttgtccgc gtcgctgctg ccgttttgcg actacgtgac cccggcgatc
     6181 gcgttctcgc cctccggcga ctacctctac acggggtgcg gcgggaccaa cgagaccaac
     6241 gtgtacgacg tgcggccgga aagtccgaac ttccatacat tgatcggcga attcgcaccc
     6301 gccggcgggg ccggcgacat cgtgtgggac atcgcgatca ccgccgacgg taaggtgggc
     6361 ctcgtcgccg gcggcatcca ggacccggcg gatccgcggc gcattcagtc gttctacgcc
     6421 gaccccaacg acccccgatt tctgacgcgc gggcccgcgg tgccgacccc cgacctccag
     6481 gccaggatcg tcagcgcgag tcccaacaac cgcaccgtcc tcacgtttgt cgaggtcgac
     6541 gagatcgtcg tgtacgacgc ggccggcatc gcggacggtg tggtgccgac ggtcgtcgac
     6601 agcacgagca cggggggtga cgtggtggac atcgcgcacc atccctccag tcgcgtggca
     6661 tacgtggcgg tggataccgg agtcatccgg atcttcgaca ccaacccgtt atctcccgca
     6721 tacgggctcg gggtgggaac cgtgtttacg gggttcgacg acatctggtc gttggcggtg
     6781 tcccccgacg gggcgaggct gtacgtgtac ggctggttca ataccgactt ggcggattac
     6841 cgcgtcctca cctacgacgc cagcactccg ccgctgttga ccaatccctt ctcctgcacg
     6901 acgaacacgg agaccgaggt cgttcagaag caggaattcg cgctcgcccc gcagggcgac
     6961 ttctccgtgc gcacggtcga aggcgtcggg tacctgcgct acaacttggt ttgcccaacg
     7021 gacgggttcc cgacggtttc cgcgcccggg tcgttgatgg aattcgcgtt ccacccgcac
     7081 ggcaatcgca tgtacgcggc ggagcagttg agctcgcgga tcggggtcta cgatttcgtg
     7141 gccgccgaga cgctcacgga agtatcgggt aacgaccagg acggtgtcgc cggcgagacg
     7201 ctgccggcgc cgctgcgtgt gcgagtgacg acgtcgatgc ccgaccgcga catgggcggc
     7261 atcccggtca ggttcgcggt cgcgaccggc ggcgggtcga tcgtgacgag cggcggtccg
     7321 gtgacgcagg ccgtggttgc gaccgacgac gacggcttcg cgcagatttt ctggaagctg
     7381 gggccggcgc tcgggccgca gacgctgggc gtatcgtcg
//
LOCUS       DASPGK010000004         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_703432, whole genome shotgun sequence.
ACCESSION   DASPGK010000004 DASPGK010000000
VERSION     DASPGK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703432"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VEC56_00070"
     CDS             <1..1782
                     /locus_tag="VEC56_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12573.1"
                     /translation="GHSNTGVHMATYCSFDKGQTWTLVPVGDAQDHLGLTPRFDPAVT
                     FDANGNVFVAYGTLVFTGSTERTSLVVAKNTDGGQTYGPAFIIGTANRVLNVPGHDKW
                     VLATGPDPTVPGRENIYIAWTWNIPTPAGNVDQQISISRSIDGGQTFSPPLVINDDSI
                     AGRDRALVADPSVGPNGEVYVSWNDIDATDATLFVDRSFDGGLTWGTDVVVSTGAMAW
                     RTPIPAQPDRGVGAAPVMDVDISGGAFTGRAYIVYCQPGANGLDILLRYSDDQAQTWS
                     LPVRVNDDATMRDQFLPWIDVGRTNGLVSVVFYDAREDPLNQSVRVYAAMSRTGGVTF
                     EPNEPVADVPSNQSVANPNRYGGNYLEYIGVATGDDAVHIVWTDARFLWPVLIDYSLR
                     FYYDGIPLESPPVPVLFSAFDAVAIEEGVRISWELWSDEAIDEFVLYRHEDASLAIAV
                     ARGAIDGATGWYVDSSVDPGKTYAYDMVVRTTDGDEFRSRVATVTTPGVTLALGQNHP
                     NPFNPRTTIPYQGPAAARPERVRLVVLDVSGRVVRTLVDEDQSSGAFRVNWDGKDDRG
                     ARVSSGVYFYLLEVGEKRRARKMVLLK"
     gene            2000..3115
                     /locus_tag="VEC56_00075"
     CDS             2000..3115
                     /locus_tag="VEC56_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12574.1"
                     /translation="MPSGPRPGDGATDQAITGGLQWIGGEAVAYDVYFGTEEDPPLVA
                     SNVPDHFYQGGTLAFATVYRWRIVARDQFGAETSGPVWSFVTRENSPPIAPFDPNPPN
                     LGLTGPAVVLGWRSGDPDLQPVTYRLFFGATNPPPQIAIGLTTRSYPIDGLQLNTTYY
                     WRVIATDGEFSTSSLVWRFHVIQLAVLISKFDAAQVGDGVDVTWELSSDEPIESLTLY
                     RRAAGESLPVPLATVDTGARVFRDETVEAGKTYHYELVVRTLDEDVYRSPVATVTMRE
                     RALALLQNHPNPFNPQTTISYDLPHVDAPQHVRLSVFDVGGRTIRTLVNQEQPSGSYA
                     VVWEGIDNYGGAVPSGVYFYVLDVDGVRRTRKMVLLK"
     gene            3141..4166
                     /locus_tag="VEC56_00080"
     CDS             3141..4166
                     /locus_tag="VEC56_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HXV12575.1"
                     /translation="MVSILISSLFLLSCAEKSAPDQRAQVSYFDNTGRDDVLSGGVKM
                     VAIDTPKGQFHVWTKRVGNNPRVKLLLLHGGPGFNHEYFECFDSFLPKEGIEYYYYDQ
                     LGSFFSDQPDDSSLWDTARFVEEVEQVRQALGLDRENFYLLGHSWGGILAMEYALKYQ
                     DHLKGLVISNMMASIPEYSAYAKSAFKSTMDPAVLAELQALEAAKDYQNPRYMELLIP
                     HHYELHIIRIPYAQWPDPVLRAFEHMNPAVYIPMQGPSELDTSGKLEKWDRVADLGKI
                     TVPTLVIGAQHDTMDPKHMEMMAGAVQRGRYLFCPNGSHMAMYDDQATYVDGVTRFIH
                     DVDEGRF"
     gene            4251..>4984
                     /locus_tag="VEC56_00085"
     CDS             4251..>4984
                     /locus_tag="VEC56_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12576.1"
                     /translation="MPRFRRATPTESARIVLFALACMAARSAGPSFAQTPTIGLYTDA
                     SGSSCNFSGDAPGMVTAYVVVRPNGTGVTAVQFAAPVPVCFGATFLTDVSVPDVLVIG
                     SSQSGVSIALLGCTNVPTHVLEISYMRTGGATAPCCAYPIVADPVVGEVAATDCAFQG
                     IPAVGVTSRFNADGSCPCTGNSPPETPTNPLPLDGAVGVSAGLGLLSWESWDVDGDSI
                     EFDVYLGTSSPPPLAASALAAPSYSMP"
BASE COUNT          867 a         1627 c         1601 g          889 t
ORIGIN      
        1 gggcactcga acacgggggt ccacatggcc acgtattgct ctttcgacaa gggacagacg
       61 tggacgctgg tgcccgtcgg tgatgcccaa gaccatctgg gacttacccc gcgcttcgac
      121 cccgcggtga ccttcgacgc caacgggaac gtgttcgtcg cctacggcac gttggtcttc
      181 acgggaagca cggagcgcac gtcgttggtg gtggcgaaga acaccgacgg cgggcaaacc
      241 tacgggccgg cgtttatcat cgggaccgcg aaccgcgtct tgaacgttcc gggtcacgac
      301 aagtgggtgc tcgctaccgg gccggatccg accgtgccgg ggcgggagaa catctatatc
      361 gcgtggacgt ggaacatccc gacaccggcc ggcaacgtcg accaacagat cagcatttcg
      421 agatcgatcg acggcgggca aacgttctcg ccgccgctcg tgatcaacga cgattcgatc
      481 gccggccgcg accgtgcgct ggtggcggac ccgtcggtgg gcccgaacgg cgaggtctac
      541 gtgtcgtgga acgacatcga cgccaccgac gcgacgctct tcgtggatcg ctccttcgac
      601 ggcggcctga cgtgggggac cgacgtggtg gtgtcgaccg gtgccatggc gtggcgcacg
      661 cccatcccgg cgcagcccga ccggggcgtg ggcgcggcgc cggtcatgga cgtcgatatc
      721 agcggcgggg cattcacggg tcgcgcgtac atcgtgtact gccagccggg cgcgaacgga
      781 ctggacatct tgctgcgcta ctcggacgac caggcgcaga catggtcgct gccggtgcgc
      841 gtcaacgacg acgccaccat gcgggatcaa ttcctgccct ggatcgacgt cgggcgcacc
      901 aatggacttg taagcgtggt gttctacgac gcgcgcgaag atccgctgaa ccagtcggtg
      961 cgcgtgtacg ccgcgatgag ccgcaccggc ggcgtcacct tcgagcccaa tgaaccggtg
     1021 gcggacgtgc cctccaatca atcggtcgcg aatccgaatc ggtatggggg caactacctc
     1081 gagtacatcg gcgtggcgac gggcgacgat gccgtgcaca tcgtctggac cgacgcgcgc
     1141 ttcctgtggc cggttctcat cgactacagc ctgcgtttct actacgacgg catcccgctc
     1201 gaatcccccc cggtcccggt gttgttctcc gcctttgacg ccgtcgcgat cgaggagggc
     1261 gtgaggataa gctgggagct gtggagcgac gaagcgatcg acgaattcgt tctatatcga
     1321 catgaggatg cgtcgctcgc gatcgccgtt gcgcgcggtg cgatcgacgg tgcgacgggc
     1381 tggtacgtgg actcgtcggt cgatcccgga aagacgtacg catacgacat ggtggttcgc
     1441 accaccgacg gcgatgaatt tcgctcgcgc gtggcaaccg tcaccacgcc cggggtgaca
     1501 ctcgcgctcg gccagaatca ccccaacccg ttcaacccgc gcaccacgat tccgtaccag
     1561 ggtcccgccg cggcccggcc ggagcgcgtg cgactcgtcg tcctcgacgt gtccggccgc
     1621 gtggtgcgca cgctggtgga cgaagaccag tccagtggcg ccttccgagt gaattgggac
     1681 gggaaggacg atcggggcgc gcgcgtgtcg agcggagtct atttctacct gctcgaggtc
     1741 ggagagaagc gccgagcgcg caagatggtg ctgctcaagt gacggtgcgg tgctgcccgt
     1801 ggcgaggatt cggacgcggg tttgttttag gtggttccgg cgtggcggcg ggtgtacgct
     1861 ccggcctcca gccaagggag gcgcgaccat gccgacgtca aacgggcgag gccctagttt
     1921 tcgaaccttc ctcacggtcc tttctgcgac gtccctcatc gtcaccctcg cgcacaaacc
     1981 ttcccatgcc gagcccgccg tgccctccgg gccgcgcccc ggagacgggg cgaccgacca
     2041 agcgatcacg ggaggtctgc aatggatcgg cggcgaggcg gtcgcttacg acgtctactt
     2101 cggcaccgaa gaagatcctc ccctggtcgc gagcaatgtt cccgaccact tctatcaagg
     2161 cggcacgctc gcgttcgcga ccgtgtatcg atggcgcatc gtcgcgcgcg atcaattcgg
     2221 agccgagacg tccggtcccg tttggtcgtt cgtgacccgt gagaattcac cgcccattgc
     2281 accgttcgac cccaatccgc cgaacttggg gctgacgggg cccgcggtcg tcctcggctg
     2341 gcgatcgggt gatcccgacc tgcagccggt cacgtaccgg cttttcttcg gggccacgaa
     2401 tccgcctccg caaatcgcga tcggactcac gacgcgctcc tacccgatcg acgggttgca
     2461 gctgaacaca acgtactact ggcgcgtgat cgcgaccgac ggcgagttct ccaccagcag
     2521 tctggtgtgg cggttccacg tcattcagtt ggcggttctg atctcgaagt tcgacgcggc
     2581 ccaggtgggt gacggcgtcg acgtgacgtg ggagctatcg agcgacgaac ccatcgaatc
     2641 gctcacgctg taccgacgcg ccgcggggga atctcttccg gtgccgctcg cgacggtcga
     2701 taccggcgca cgcgtgtttc gcgatgagac cgtggaggcg gggaagacgt accactacga
     2761 gctggtcgtg cgaacgctcg acgaggacgt gtaccgatcg ccggtcgcga ccgtgacgat
     2821 gcgcgagcgc gcgctggcgt tgctgcagaa ccaccccaac ccgttcaatc cgcaaacgac
     2881 gatttcgtac gaccttcccc acgtcgatgc gccccagcac gtgcgactgt cggtattcga
     2941 cgtcggcggt cgcacgattc ggacgctggt gaaccaggaa cagccgagcg gctcgtacgc
     3001 ggtcgtgtgg gagggcatcg acaactatgg cggcgcggtc ccgagcgggg tgtacttcta
     3061 cgtgctcgac gtcgacggcg tacgacggac gcgtaagatg gtgctgctca agtgacagag
     3121 gtgatcataa cgtgacccgg atggtatcga tcctaatctc ctcgttgttt ctcctttcct
     3181 gcgcggagaa gtccgcgccc gaccaacgcg cgcaggtctc ttacttcgac aacaccggac
     3241 gcgacgacgt cttgagcggc ggcgtcaaga tggtcgcgat cgacacgccc aaaggtcaat
     3301 tccacgtgtg gacgaaacgc gtcggtaaca acccgcgggt gaaattactc ctcctgcacg
     3361 gcggtcccgg gttcaaccac gagtacttcg agtgcttcga cagctttctt ccgaaggagg
     3421 gcatcgagta ctactactac gaccagctcg gttccttctt cagcgaccaa cccgacgatt
     3481 cgtcgttgtg ggatacggcg cgcttcgtcg aggaagtcga gcaagttcgc caagcgctcg
     3541 gcctcgatcg cgagaacttc tacctgctcg gtcactcgtg gggcggaatc ctcgcgatgg
     3601 agtacgcgct caagtatcaa gatcatctga aaggtctcgt catctccaac atgatggcga
     3661 gcattcccga gtacagcgcg tacgccaagt cggccttcaa gtcgaccatg gacccggccg
     3721 ttctcgccga acttcaggcg ctggaggcgg cgaaggacta ccagaacccg cgctacatgg
     3781 aactcctaat cccgcaccat tatgaactgc acatcattcg cattccgtac gcgcagtggc
     3841 cggacccggt gctgcgcgcg ttcgagcaca tgaatcccgc ggtctacatc ccgatgcagg
     3901 ggccgagcga gctggacacg agcggcaagc tcgagaagtg ggatcgcgtc gcggacttgg
     3961 ggaagattac ggtgcccacc ctcgtcatcg gcgcgcagca cgacaccatg gacccgaagc
     4021 acatggagat gatggcggga gcggttcagc gcgggcgcta tctcttctgc ccgaacggga
     4081 gccacatggc gatgtacgac gaccaggcga cctacgtgga cggcgtgacc cgcttcatcc
     4141 atgacgtcga cgagggacgt ttttgaacga aaaagggctt cgacccttgt cccctccggc
     4201 acacgggtgt atcctgggag cgcccccggc gaccgggagt cgccagcgcc atgccgaggt
     4261 tccggcgagc cactcctacg gaaagcgcgc gcatcgttct gttcgcgctc gcgtgcatgg
     4321 ctgcgcgcag cgcaggcccc tccttcgcac aaactccgac gatcggcctc tacaccgacg
     4381 catcgggttc cagttgcaac ttctccggcg acgctcccgg catggttacg gcctacgtcg
     4441 tggtgcgccc caacggcacc ggtgttaccg cggtgcagtt cgcggcaccg gttcccgtgt
     4501 gcttcggggc cacgttcctc accgacgtct cggtacccga cgtcctggtg atcggctcct
     4561 cgcagtccgg cgtgtcgatc gcgctcctgg gctgcacgaa cgtcccgacg cacgtcctcg
     4621 aaatcagcta catgagaacc ggcggtgcca ccgccccgtg ctgcgcatac ccaatcgtcg
     4681 cggatccggt cgtgggcgag gtcgcggcca ccgactgcgc gttccaggga atccccgcgg
     4741 tcggggtcac gagccggttc aacgccgacg gatcgtgccc gtgcaccggc aacagcccac
     4801 cggaaactcc caccaacccg ctcccgctcg atggcgcggt tggagtgagc gcggggctcg
     4861 gactgctttc ctgggagtcg tgggacgtcg acggtgactc gatcgagttc gacgtttact
     4921 tggggacgtc ctcaccgcct cctctggcgg cgagcgcgct cgcggctcct tcctactcga
     4981 tgcc
//
LOCUS       DASPGK010000005         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_352312, whole genome shotgun sequence.
ACCESSION   DASPGK010000005 DASPGK010000000
VERSION     DASPGK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352312"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VEC56_00090"
     CDS             complement(<1..1625)
                     /locus_tag="VEC56_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV12577.1"
                     /translation="MTTKRIRCAIFGGGEGDLFVFGELHKRRDVEIAFVYDRYPAAVG
                     LEIAEILRVPRASTPEEMGAFLPIDVAVVGDPRDRFARELEMLGDAEVLDHTQALSRI
                     AKHPQPKPRAEAKTVGVGEKVPYSIDDALAGFERLFDRKRLLKFLLDVAVQTTGATNG
                     SIMLYSPEARELYIAYATGLSERVVKNTRQKLGEGIAGGVARDRKGKLIRQTAVGALY
                     ESGRDRVAIASACSVPLLDPEDGGERLLGVLNVSSTKNARELSMEDLATLERLSRRMA
                     RVLAESLKLQEVQLRSGEMNLRQSVGELAEKPGTTAEKFGLLASLVAKLTGAESVEVF
                     VSAPGGEWLVLGGSNRRVANEPELVRVGRGALARAFLEKRAIILTEPVDPQSMELASS
                     FVFVPLVLTEVVGVLTMEFADRARLDDFIAVKDSVALELARFIGSERRERRLKSELAS
                     LAKVSEAAPALIACRSLQDLADVVVRVTADALTCERVSMRVRARAEDAWTIARYDAEG
                     RAQEAWLAEDDETFAKLERSRAPFQTAVLHFDG"
     gene            complement(1785..>3210)
                     /gene="mutS"
                     /locus_tag="VEC56_00095"
     CDS             complement(1785..>3210)
                     /gene="mutS"
                     /locus_tag="VEC56_00095"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXV12578.1"
                     /translation="DRAGALLGDHAALCDTISRAIVADPPSHLRDGGVIRKGFDAGLD
                     SLIDESEAAKRWIAGLEARERERTGISSLKVGYNKVFGYFIEVSNAHKNSIPPDYVGK
                     QSLVNGQRFFTPELKEREDLILSTEERRVREENRVYDELCAQVASHAEALQRAARGIA
                     TIDAVLSLAMVARARGYRRPVVDETSIIEIVGGRHPVLERVVTEPFVPNDVHLDPERR
                     QFALITGPNMSGKSTFLRQTALIVLMAQMGSFVPADRARVGLVDRIFTRVGASDRLSR
                     GESTFLVEMNETANILHHMTDKSLLILDEIGRGTSTYDGLAIAWAVAEYVLQGVVARP
                     RTLFATHFHELTQLKSTYPRLVNLKIAIKEWEGGIVFLRKIVAGTSDKSFGIHAAKVA
                     GLPPLVIKRAEEILESLELRRDLVARGMDSIDANSRDQMSLFNPSTPPGGGDIARAID
                     AFDIDRSTPLDALDLIRRLKDRPR"
BASE COUNT          510 a         1102 c         1070 g          528 t
ORIGIN      
        1 ctcccgtcga agtgaagcac cgcggtctgg aacggcgcgc gcgagcgctc gagcttcgcg
       61 aacgtctcgt cgtcttcggc cagccacgcc tcctgcgcgc gtccctcggc gtcgtagcgc
      121 gcgatggtcc acgcgtcctc ggcgcgcgcg cgcacccgca tcgatacgcg ctcgcacgtc
      181 agcgcgtcgg ccgtcacgcg caccaccacg tcggccaggt cctgcagaga tcggcacgcg
      241 atcagcgcgg gtgcggcctc cgaaaccttc gcgagcgacg ccagctcgct cttgaggcga
      301 cgctcgcgcc gctccgagcc gatgaagcgc gccaactcga gcgccaccga atccttgacc
      361 gcgatgaagt cgtccaggcg cgcccggtcc gcgaactcca tggtcaggac accgaccact
      421 tcggtgagca ccagcggcac gaacacgaac gacgacgcca gctccatcga ctgggggtcg
      481 acgggctcgg tcagaatgat ggcgcgcttc tcgaggaacg cgcgcgccag ggcaccgcgg
      541 cccacgcgca cgagctcggg ttcgttggca acccggcggt tcgagccgcc cagcacgagc
      601 cactcgcccc cgggcgcact cacgaatact tcgaccgatt ccgcccccgt caatttcgcg
      661 accaacgacg ccaacaggcc gaacttctcc gccgtggtgc cgggcttctc ggccagttcc
      721 cccaccgact ggcgcaggtt catctcgccc gagcgcagct gcacttcctg gagcttgagc
      781 gactccgcga gcacgcgcgc catacgtcgg ctcaagcgct cgagagtggc caggtcttcc
      841 atcgagagct cgcgcgcgtt cttcgtggac gacacgttga gcacgccgag caagcgctcg
      901 ccaccgtctt cggggtcgag cagcggtacc gagcacgccg acgcgatggc gacgcggtcg
      961 cgcccggatt cgtagagcgc cccgaccgcg gtctgccgga tcagcttgcc cttgcgatca
     1021 cgcgccacac cgcccgcgat cccttcgccc aacttctggc gtgtattctt gaccacgcgc
     1081 tcgctcaacc cggtcgcgta ggcgatgtag agctcgcgcg cctcgggcga gtacagcatg
     1141 atcgagccgt tggtcgcacc ggtggtctgc acggcgacgt cgaggaggaa cttgaggaga
     1201 cgcttgcggt cgaagaggcg ctcgaagccg gccaacgcgt cgtcgatcga gtagggcacc
     1261 ttctcgccca cgccgaccgt cttcgcttcc gcgcgcggct tgggctgcgg gtgcttcgcg
     1321 atgcgcgaga gcgcctgggt gtggtccagg acctcggcgt cgccgagcat ctcgagctcg
     1381 cgcgcgaagc gatcgcgcgg gtcgcccacg acggccacgt cgataggcaa gaatgccccc
     1441 atttcctcgg gggtcgaggc gcgcggaacc cgcagaatct ccgcgatctc caggcccacc
     1501 gcggccggat accggtcata gacgaatgcg atctcgacgt cccggcgctt gtggagctcg
     1561 ccaaagacga agaggtcgcc ctcgccaccc ccaaagatag cgcagcgtat tcgctttgtg
     1621 gtcattgtgt tagatagagc acttcgagtg ctttcgacgc tccgtccgcc ggcggacgga
     1681 ggcgtcctca tggggagagc aaatcgcgga ccatgactcc ccgtcgtgcc cggggattac
     1741 gtggtggctt ccccccggcg ggtgtcactt cgtgggggcc ggcgtcagcg cgggcggtct
     1801 ttgaggcggc ggatcagatc gagggcgtcc aggggagtcg agcggtcgat gtcgaaggcg
     1861 tcgatggcgc gcgcaatatc gccgccgcca ggcggcgtcg atgggttgaa caagctcatc
     1921 tggtcacgtg aattggcgtc tatggaatcc atgccccgcg cgaccaaatc ccgccgcagc
     1981 tcgagtgatt ccaagatctc ctcggcgcgc ttgatgacca acgggggcag ccctgcgacc
     2041 ttggccgcgt ggatgccgaa gctcttgtcg ctggtgccgg cgacgatctt gcgcaggaac
     2101 acgatgccgc cctcccactc cttgatcgcg atcttgaggt tgaccaggcg cgggtaggtc
     2161 gacttgagct gggtcagctc gtggaagtgg gtcgcgaaca gcgtacgcgg tcgcgccacg
     2221 actccctgca gcacgtactc ggcgaccgcc cacgcgatcg cgagcccgtc gtaggtggag
     2281 gtgccgcgcc cgatttcatc gaggatcaag aggctcttgt cggtcatgtg gtgcaggatg
     2341 ttggcggtct cgttcatctc gacgaggaac gtcgattcac cgcggctcag gcggtcagag
     2401 gcgccgacgc gcgtgaagat gcggtcgacg agccccacgc gcgcgcggtc ggcggggacg
     2461 aacgacccca tctgcgccat cagcacgatg agcgcggtct ggcgcaggaa cgtgctcttg
     2521 cccgacatgt tgggtcccgt gatgagcgcg aactgccggc gctccggatc gaggtggacg
     2581 tcgttgggga cgaagggttc ggtgacgacc cgctccagca cggggtggcg tccgcccacg
     2641 atctcgatga tcgaggtctc gtcgaccacc gggcgccggt acccgcgtgc gcgcgcgacc
     2701 atggccaggg acagcaccgc gtcgatggtc gcgatccccc gtgccgcgcg ctggagcgct
     2761 tccgcgtgcg acgccacctg tgcgcacagc tcgtcataca cgcgattctc ttcgcgcacg
     2821 cggcgctctt cggtgctcaa gatcaagtcc tcgcgctcct tgagctccgg ggtgaagaag
     2881 cgctggccgt tgaccagcga ctgcttgccg acgtagtcgg gcgggatcga gttcttgtgc
     2941 gcgttcgaga cctcgatgaa gtacccgaag accttgttgt agccgacctt gagcgaggag
     3001 atgccggtgc gctcacgctc gcgcgcttcc aagcccgcga tccagcgctt ggccgcttcg
     3061 ctctcgtcga tcagcgaatc gagtcccgcg tcgaagccct tacgaatcac gccgccgtcg
     3121 cgcaggtgcg agggcgggtc ggccacgatg gcgcgcgaga tggtatcgca cagcgcggcg
     3181 tgatcgccca agagcgcccc cgcgcggtcg
//
LOCUS       DASPGK010000006         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_469922, whole genome shotgun sequence.
ACCESSION   DASPGK010000006 DASPGK010000000
VERSION     DASPGK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469922"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VEC56_00100"
     CDS             <1..1340
                     /locus_tag="VEC56_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXV12579.1"
                     /translation="SRATLAAPRAPGILREAWRRAGDQPQSNWGQTAAVVDIDGDGFS
                     ELIAGESLWIGADSVARGRVVVFPGTADGPSAEGKTVLTGDVRGFAYGHYAADAGDVD
                     GDGVHDFLITEVPYKMEEGPGHVFLFHGGPGPGPLTPWWTHQGQSRDSGFGLSMMSGD
                     VNGDGYSDVVIGEPVATDRIDREGIVRMFLGSRSGLSSEPAWTVRGEQAVAQLGGWMR
                     TTGDVNGDGFDDVLVTAPFWDGATTDCGQARLYLGGKDGVAEEPAWTAEGSGTNSHFG
                     TTVSGAGDVNGDGFGDVIIGEPQYSDASRPERGRVLLFLGGREGLSKTPAWQALGPIA
                     YMHVGYSVLGLGDIDGDGLADIAVGAPQYTEGKHTHLGVVEVYRGTREGCEATAAWRA
                     IGTAADEHFGFTMAVGDMNGDHIPDLIVSAPLFTDTLRERGLLISYLGQRLPK"
     gene            1543..>2254
                     /locus_tag="VEC56_00105"
     CDS             1543..>2254
                     /locus_tag="VEC56_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12580.1"
                     /translation="MTLVPAGQYTPILVPDGSGGAIVVWQDDRNGASDIYAQRVNSRG
                     TSLWAANGVALCTAPGAQWVLAQWAAIVADGAGGAIVVWQDLRSDAGDVYAQRVNSAG
                     VPLWAANGVALCAAANAQEFPVAIADGSGGVVVAWQDYRSGANYDVYAQRVNAVGAVQ
                     WVANGVLMCADGTDQDSHQITSDGTGGAIVAWRDWRYGASDHDIFAQRINGAGVLQWF
                     GSGNSMCNALGNQQAPQLV"
BASE COUNT          365 a          703 c          795 g          391 t
ORIGIN      
        1 tttcgcgcgc gacactcgcg gcgccgcgag cgccgggcat actccgagaa gcgtggcgac
       61 gggccggcga tcagccgcaa tcgaactggg gacagaccgc agcggtggtc gatatcgacg
      121 gtgatgggtt cagcgaattg atcgcgggag aatcgttgtg gatcggtgcg gacagcgtcg
      181 cgcgcgggcg cgtggtcgtg ttccccggca ccgccgacgg accgagcgcc gagggaaaga
      241 cggtgctcac cggggatgtt cgcggctttg cgtacgggca ctacgccgcc gacgccggtg
      301 atgtcgacgg cgacggcgtt cacgattttc tcatcaccga agttccgtac aagatggagg
      361 aaggcccggg ccacgtattc ttgtttcatg gcggccctgg ccctggtccg ctgaccccgt
      421 ggtggactca ccagggacaa agccgcgact ccggattcgg cctgtcgatg atgtcgggcg
      481 acgtgaacgg cgacggatac agcgatgtcg tgatcgggga gccggtcgcc accgatcgca
      541 tcgaccgcga agggatcgtg cgcatgttcc tgggctcgcg gtcggggttg tcatccgagc
      601 ccgcgtggac ggtgcgcggc gagcaggcgg tcgcacaact gggtggatgg atgcgcacga
      661 cgggcgacgt caacggcgac ggattcgacg acgtactggt gacggcgcca ttttgggacg
      721 gcgccacgac ggactgcggc caggcgcggc tctacctcgg cggcaaggat ggtgtcgccg
      781 aagagcccgc gtggaccgcc gaaggatccg gcaccaattc gcacttcggc accacggtgt
      841 cgggcgccgg cgacgtcaac ggcgacggct tcggcgatgt catcatcggc gagccgcagt
      901 actccgacgc gtcgcgcccc gagcgtggtc gtgtgttgct ctttctcggc ggcagggaag
      961 gactctcgaa gacacccgct tggcaagcgc tgggacccat cgcgtacatg cacgtcgggt
     1021 attcggtctt gggtctcgga gacatcgacg gcgacgggct ggctgacatc gccgtgggtg
     1081 cgccgcaata caccgagggc aagcacacac atttgggtgt ggtcgaagtg tatcgcggga
     1141 cgcgcgaggg ctgcgaagcc acggcggcgt ggcgcgcgat tggtaccgcc gccgacgaac
     1201 acttcggctt caccatggcc gtcggcgaca tgaacggcga ccacattccc gatctgatcg
     1261 tctccgcgcc gctgttcacc gatactctgc gcgagcgagg gctgctcatt tcctacctag
     1321 gtcagcggct ccccaagtaa ttcacaaacc ccctgttcgc tttcgtgctc gccggcattt
     1381 cgttgggatg acgggcgatt gtgcctcgct tcgcctgcga ggccgcgact cgcccctggc
     1441 acggaggttt ccatgaaaag ccggtcgttt agacttgccc taatctcgat cgtattcctt
     1501 cccagtctct cgtttgcgca atgggcgccg gacggcgctc ctttgaccct cgtaccagcc
     1561 ggccagtaca caccgatcct cgtccccgat ggctcgggcg gtgccatcgt ggtgtggcag
     1621 gacgatcgca acggcgccag cgatatctac gcccagcggg tgaactcgag gggcacgtcg
     1681 ctgtgggctg cgaacggcgt agcgctctgc acggcgccgg gcgcccagtg ggttctcgct
     1741 caatgggcag cgatcgttgc cgacggcgcg ggtggcgcca tcgtcgtgtg gcaggacctt
     1801 cgcagcgacg ctggcgacgt gtacgcccag cgcgtgaact cggccggcgt gcccctgtgg
     1861 gcggcgaacg gagtcgcgtt gtgcgccgcg gcgaacgcac aagagttccc ggtggcgata
     1921 gccgacggtt ccggcggtgt cgtcgtcgcg tggcaggact atcgcagcgg agccaactac
     1981 gacgtctacg cgcagcgcgt taacgcggtg ggcgcggtcc agtgggtcgc gaacggagtc
     2041 ctcatgtgtg cggacggcac cgaccaggat tcgcaccaga tcacctcgga cgggaccggc
     2101 ggggcgatcg ttgcgtggcg tgactggcgc tatggtgcgt cggatcacga catcttcgcc
     2161 cagagaatca atggggccgg ggtgctccag tggttcggga gcggcaacag catgtgcaac
     2221 gcgctcggga accagcaagc cccgcaactc gtct
//
LOCUS       DASPGK010000007         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1289931, whole genome shotgun sequence.
ACCESSION   DASPGK010000007 DASPGK010000000
VERSION     DASPGK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289931"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VEC56_00110"
     CDS             complement(<1..159)
                     /locus_tag="VEC56_00110"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXV12581.1"
                     /translation="MATKTRCTWPGTDPLMLSYHDEEWGTPLHDDRALFEFLILEGAQ
                     AGLSWRTIL"
     gene            complement(209..865)
                     /locus_tag="VEC56_00115"
     CDS             complement(209..865)
                     /locus_tag="VEC56_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12582.1"
                     /translation="MTPLGPYNIADQACPDHIVQVAGPEWGDWANLPHWADPQGPHCA
                     GSPPASLLLLEGPPDHPRNVYGIYDLRPDITFELRFVNNTVANLPGPDLVFFQMDQFN
                     EFQLPTRVPGGYMIALEDGAGGFGAFHIYPKELAVPIGETWGYILCGPEHGPAGFPIS
                     MIEIDLSDFGVEEGVSITALRFSALDLADPLIVAAVHSANPVAVESTTWGRIKAMYRH
                     "
     gene            complement(1051..2028)
                     /locus_tag="VEC56_00120"
     CDS             complement(1051..2028)
                     /locus_tag="VEC56_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXV12583.1"
                     /translation="MAPDKRMLTNDGAAFDPVTAFTTPTLEFDFPGLEVGVAEYAEGP
                     TGCTVFSFPRGATCVADVRGGAPGTILTHREGWVDAICLAGGSVYGLEAASGVNAELL
                     ARKNYDTDWNNIAIVSGAVIFDFNRFRANKAVYPDKALGRAALRASRPGMFPLGPRGA
                     GVCASVGKWLRDAYELERSGQGAAVYASGDARVAAFTVVNAVGALVDRNGRAVRGHLD
                     PKTGRRVRVGEVVAIRREAEPMPEPPPGNTTLTVIVTNQTMGTRELNQMARQVHSSMA
                     RAIDPFHTMYDGDVLYCVTTNTLAPAPLNYNEVSYVASELAWDAVLRSF"
     gene            2148..>3245
                     /locus_tag="VEC56_00125"
     CDS             2148..>3245
                     /locus_tag="VEC56_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HXV12584.1"
                     /translation="MRKNLSAALLLAATALLASCAKNVEKDAQAFVDTYTQEFVKLGL
                     AVSEAEWASNTHIVEGDTTNAYNSKVAKEAYAAFTGNAANIEQAQKFLESRDKLSPLL
                     VRQLDAILYEAGNNPQTVADIVKKRIKVETEQVEKLYGFDFQLDGKSVTTNDIDELLR
                     NDNDLAKRLAAWEQSKEVGKNLRAGLAEMQTLRNQTVQALGYSDYFAYQVSDYGMTTQ
                     ELRDQMQQYMRELWPMYRELHTWARYELAKKYSVEEVPEMIPAHWLPNRWGQDWSAMV
                     TVEGLDLDSKLKEKEAEWLVKQAESFYVSLGFGGLPQTFWDKSSLYPVPEGETYKKNN
                     HASAWHFDYDKDVRCLMSVIPNADWYETSHHE"
BASE COUNT          637 a         1042 c         1037 g          529 t
ORIGIN      
        1 gagaatagtt cgccagctca accccgcttg cgcgccttcg aggatcaaaa actcgaagag
       61 tgcgcggtcg tcgtggagcg gcgtacccca ctcctcgtcg tggtacgaca gcatgagagg
      121 gtcagtgcct ggccaggtgc agcgggtctt ggttgccatg cgattcgtct acggcattgg
      181 actcatcgcg tcaagagccc gcgtgcggtc aatgacggta catcgccttg atgcgtcccc
      241 atgttgtcga ctcgacggcg accgggttcg cggaatgaac cgccgccacg atgagcgggt
      301 cggccaggtc gagcgccgag aaccgaagcg ccgtaatcga cacgccctcc tccaccccga
      361 agtccgacag gtcgatttcg atcatcgata tcggaaagcc ggccgggccg tgctccgggc
      421 cgcaaaggat gtatccccag gtctcgccga tgggcacggc gagttccttc ggatagatgt
      481 ggaacgcgcc gaacccgccc gcgccgtcct cgagcgcaat catgtatcca cccggcacgc
      541 gcgtcggtaa ctggaattcg ttgaactgat ccatctggaa gaagacgagg tcgggtcccg
      601 gtaaattcgc gaccgtgttg ttcacgaaac gcagctcgaa ggtgatgtcc ggcctgagat
      661 cgtagatccc gtacacgttg cgagggtggt ccggcgggcc ctcgagcaac aaaagactcg
      721 ccggcgggga cccggcgcag tgcggcccct gcgggtcggc ccaatgcggc aagtttgccc
      781 agtcccccca ctcggggccc gcaacctgca caatgtggtc tggacaggct tgatcggcga
      841 tgttgtaggg gcccagcgga gtcatgccgg ccgacgcgcg aggcacggcc gatagtgcaa
      901 ggagagtcca tccgagaatc acgcgagttt gcatcgcgcc acctccagcg gaatcgcagt
      961 cggggattgg gcggcgagga tcattatacc acgatgtcac gccgccaaac gacccgctct
     1021 ctaccgggca aactacacag gatcacacgt tcagaagctt cgcaacaccg cgtcccaggc
     1081 cagctcggac gcgacgtagc tcacttcgtt gtagttcaac ggcgcgggag cgagtgtgtt
     1141 ggtggtgacg cagtacagca cgtcgccgtc gtacatggtg tggaacgggt cgatggcgcg
     1201 cgccatcgag ctgtggacct ggcgcgccat ctgattcaac tctcgagtcc ccatcgtctg
     1261 gttggtgacg atcacggtga gggtcgtgtt gcccggcggc ggctcgggca tgggctcggc
     1321 ctcgcggcgg atcgcgacga cttcgcccac gcgcacgcga cgtcccgtct tggggtcgag
     1381 gtggccgcgc accgcgcgcc cgttgcgatc gaccagtgct cccaccgcgt tgaccaccgt
     1441 gaaggccgcc acgcgcgcgt cgccggacgc gtataccgcg gcgccttgac ccgaccgctc
     1501 gagttcgtag gcgtctcgga gccattttcc gaccgacgcg cacacgcccg ccccgcgcgg
     1561 ccccagcggg aacatacccg gccgcgacgc gcgcagcgcc gcgcgaccga gcgccttgtc
     1621 gggatacacg gccttgttgg ctctaaagcg attgaagtcg aagatgacgg cacccgacac
     1681 gatcgcgatg ttgttccagt cggtgtcgta gttcttgcgc gcgagcaact ccgcgttcac
     1741 accgctggcc gcttcgaggc cgtacacgga tccgcccgct agacaaatcg cgtccaccca
     1801 cccctcgcgg tgggtgagga tggtgcccgg ggcaccgccg cgcacgtcgg ccacgcacgt
     1861 cgcaccgcgc ggaaaggaaa acaccgtgca gccggtgggg ccttccgcgt actcggccac
     1921 accgacttcg agcccgggga aatcgaactc gagcgtggga gtggtgaacg cggtgacggg
     1981 gtcgaacgcg gcgccgtcgt tggtcagcat tcgcttgtcg ggtgccatgc cgcgtcatta
     2041 tagcattggc acgttgggtg gaaatcagct agtatccgcg aacttgccgc ccggaccccc
     2101 acccaagcgc ggcgctatcc catacgagtt cccactcagg aggcaacatg aggaagaatc
     2161 tctcggccgc ccttctcctc gcggccacgg cacttctcgc ttcgtgcgcg aagaacgtcg
     2221 agaaggacgc gcaggcgttc gtcgatacct acacccaaga attcgtcaag ctggggctgg
     2281 ccgtcagcga agccgagtgg gcgtcaaaca cccacatcgt cgaaggcgac accaccaacg
     2341 catataactc caaggtggcg aaggaagcct acgccgcctt caccggcaac gcggcgaata
     2401 tcgagcaggc gcagaagttc ctggagagcc gcgacaaact ttcgccgctc ttggtccgcc
     2461 agctcgacgc catcctctac gaggccggca acaaccccca gaccgtcgcc gacatcgtga
     2521 agaagcgcat caaggtggag accgagcagg tcgagaagct gtacggcttc gacttccagc
     2581 tcgacggcaa gtcggtcacc accaacgaca tcgatgagct cttgcgcaac gacaacgacc
     2641 tcgccaagcg gctcgcggcg tgggagcaga gcaaggaagt cggcaagaac ttgcgcgcgg
     2701 gcttggcgga gatgcagacc ttgcgcaacc aaaccgtgca ggcgctgggt tacagcgact
     2761 acttcgcgta ccaagtctcc gactacggca tgacgaccca ggagctgcgc gatcagatgc
     2821 agcagtacat gcgcgagctg tggccgatgt accgcgagct gcacacctgg gcccgctacg
     2881 agctggccaa gaagtacagc gtcgaggaag tgccggagat gatcccggcg cactggctgc
     2941 cgaatcgctg gggccaggat tggtcggcca tggtcacggt cgagggtctc gacctcgatt
     3001 ccaagctcaa ggagaaggaa gcggagtggc tggtgaagca agccgagagc ttctacgtga
     3061 gcctcggctt cggcgggctc ccgcagacgt tctgggacaa gtcgtcgctg tatccggtcc
     3121 ccgaagggga gacgtacaag aagaacaacc acgcgtcggc gtggcacttc gactacgaca
     3181 aggacgtgcg ctgcttgatg agtgtgatcc ccaacgccga ttggtacgag acatcgcacc
     3241 acgag
//
LOCUS       DASPGK010000008         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_821784, whole genome shotgun sequence.
ACCESSION   DASPGK010000008 DASPGK010000000
VERSION     DASPGK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821784"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..1105)
                     /locus_tag="VEC56_00130"
     CDS             complement(407..1105)
                     /locus_tag="VEC56_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV12585.1"
                     /translation="MSSPIEALSDTCRGMLLHLKEHGGATIAELAEHIGISDEGARQH
                     LLRMERKGWIQRRETRPTGGRAGRPAAVYEVSPSGEAFFPKRYDELSIALADILVETQ
                     GPAALQSALARITDAWVDAWAPRLAGKSLDERLALLKDYYLPGDPFLTIERNGNVSLV
                     EHNCPFLNVAMARPALCSITVNALTRLLGRRVYRHRKFQNGDGCCEFRVTPQAVEAEQ
                     HTFALEKPSETENS"
     gene            1281..>2258
                     /locus_tag="VEC56_00135"
     CDS             1281..>2258
                     /locus_tag="VEC56_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXV12586.1"
                     /translation="MRIALVQFNPIVGDVEGNLARILRQVDDARARGAELVVFHELAV
                     SGYPPQDLLERRDFLAQCEAALERVREASARFPGLGIVIGVPLPARSGPGKGVANCAL
                     LIEGGQIRHRHDKLLLPTYDVFDEARHFEPGATAVPVEFGGERLGISVCEDAWNDPVL
                     CRPPLYRVDPIAMLAQAGATLMVNISASPFSVGKEAFRLDLVRSHCRRHRVPFVMVNQ
                     VGGNDELVFDGCSLFVGADGAVRAALPAFEEAVHLVDTRSGAPAIEHRALPDLESIHR
                     AIVLGVRDYFAKCGFRRAVVGLSGGVDSALTLCFAVEALGAANVLAVAMP"
BASE COUNT          356 a          772 c          735 g          395 t
ORIGIN      
        1 tgacgatgac accccaagca tgtaaaccgc tggatacgat gcggggagcg ttgacgcagg
       61 aagaagccct tgcgcttgaa cggccacgcg ggcgacaacg tgttgaagta tgtgcagttg
      121 gcgttgggac aatgtggagg cgcccatccc gtcgttccca gtcccatact tcgtccccgg
      181 cacatcccgt gccagggaca atctcgcggt cacaaccgaa atcttcaaat tcgattagcc
      241 gtcgcgtccg gaaacggcgc actacccctc aagtcaggta gcgggggaag gcggctcgcc
      301 gaagcccggc aagaaacaac cttaatacgg tcggcggttt ttcgccgcgg accggcgagc
      361 cgtttccacc gttttcggcg tttcccaccc gaccatcacc cggccctcac gaattctccg
      421 tctcgctcgg tttctccagc gcgaacgtgt gctgctcggc ttcgacggcc tgcggcgtca
      481 cgcggaactc gcagcatccg tcgccgttct ggaatttgcg atgccggtac acgcggcggc
      541 cgagcagccg tgtcagggcg ttcacggtga tgctgcacag cgcgggacgc gccatggcca
      601 cgttcaagaa cgggcagttg tgctcgacga gcgaaacgtt gccattgcgc tcgatggtga
      661 ggaagggatc gccggggagg tagtagtcct tgagcaacgc cagccgctcg tcgagggact
      721 tgcccgcgag ccgcggcgcc cacgcatcca cccacgcgtc ggtgatgcgc gcgagcgccg
      781 actgcagcgc ggcgggacct tgcgtctcga ctagaatgtc ggccagcgcg atcgagagct
      841 cgtcgtagcg tttgggaaag aacgcctctc cggatgggct gacttcgtac accgcggccg
      901 gccggccggc gcgaccgccc gtgggccgcg tctcacgccg ctgaatccag ccctttcgct
      961 ccatgcggag caagtgctgg cgggcgcctt cgtcggaaat gccaatgtgc tcggccagct
     1021 cagcgatcgt cgccccgccg tgctctttca agtggagcag catgccccgg caggtatccg
     1081 acaaggcctc gattggactg ctcacgtcgc ctccagacac cagccaccgc ccgtatcggc
     1141 gcccggcgtc gaacattcgt gggttcggga ttaaccaagc gtagggcttc catacccgca
     1201 ggtcaacgat taggacgagt ctaacttggg gaagctccga ttcacggagg ccgaatcggg
     1261 ctgtatactc ccaccgaccc atgcgaatcg ccctcgtgca gttcaatccc atcgtcggcg
     1321 acgtcgaagg caacctggcg cgcatcctcc gccaggtcga cgacgcgcgc gcccgcggcg
     1381 cggagctcgt cgtttttcac gaattggcag taagcgggta tcccccccag gatctcctcg
     1441 agcggcgcga cttcctcgcc cagtgcgagg ccgccctcga gcgcgtgcgc gaggcgtccg
     1501 cgcgtttccc gggcctcggc atcgtgatcg gcgtgccgct cccggcgcgc tccgggcccg
     1561 gcaagggtgt tgccaactgc gcccttctga tcgagggcgg gcagatccgg caccgccacg
     1621 acaagctcct gcttcccacc tacgacgtgt tcgacgaagc gcgccacttc gagcccggtg
     1681 ccaccgcggt accggtcgag tttggcggcg agcggctcgg catctcggtg tgcgaggacg
     1741 cctggaacga ccccgtgttg tgccggccgc ccctgtaccg cgtcgacccg atcgccatgt
     1801 tggcccaagc cggcgcgacc ctgatggtca acatctccgc ctcgccgttc tcggtgggca
     1861 aggaggcgtt ccgcctcgat ctggtgcgca gccattgccg ccgtcatcgc gtcccgttcg
     1921 tgatggtcaa tcaggtggga ggaaacgacg agctcgtgtt cgacgggtgc agcctcttcg
     1981 tgggcgccga cggcgccgtg cgcgcggcct tgcccgcgtt cgaagaagcc gtgcacctcg
     2041 tcgacactcg gtcgggcgcg cccgcgatcg agcaccgcgc gctcccggac ctggaaagca
     2101 tccaccgcgc catcgttctg ggcgtgcgcg actacttcgc caagtgcggg ttccggcgcg
     2161 cggtggtcgg cctgtcgggc ggggtcgact ccgcgctgac gttgtgcttc gcggtcgaag
     2221 cgctgggggc cgcgaacgtg ctcgcggtcg cgatgccg
//
LOCUS       DASPGK010000009         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1641866, whole genome shotgun sequence.
ACCESSION   DASPGK010000009 DASPGK010000000
VERSION     DASPGK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641866"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VEC56_00140"
     CDS             <1..1372
                     /locus_tag="VEC56_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12587.1"
                     /translation="IEINPIIIDLVTNRYRDYVGDAFHDPRVRLVQDEGRSCIARSRE
                     RFDVIQITLIDTWAASVSGAYSLSENYLYTTEAFAEYISHLSDGGLLSITRWYYEMPR
                     LVALARAGLEETGVEAPAHHVLVVHEKIRALLLVKHSPFTPDEVARAREYAHAMGWTV
                     AHDPVQPAPNAPYTNLLTAPDPTTIFKDADVALHPVRDDSPFFFQMTRWKSVKLDALR
                     NFGGVGFLEPLAIPVAQIALLGALVISLVLSAVLIAVPLVVKKGRAVPRENRWTWLVY
                     FLCLGLAFIVVEVVLMQRFALFLGHPTYSVTVVLFAILLFSGLGAAWSDRRGGTPAEV
                     AKPVMYWLPIAIVALAFVVPIVLQKLIGLPHAARLVIAVAMIAPVAFLMGVPFPLGIR
                     AIAATGGKHVPWAWAANGCASVVGSVCAVLGAMVWNFSTMLLIAGAIYVLALVVLARI
                     KTPLSA"
     gene            complement(1380..3398)
                     /locus_tag="VEC56_00145"
     CDS             complement(1380..3398)
                     /locus_tag="VEC56_00145"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV12588.1"
                     /translation="MHRRSVLGVLIVGVALVGVRNTPSAPPVMDDQGAPRCAESKAAL
                     SFRSVREPFSGASTNIDVGYYHLSLTLNFAPDSIAGVVRVEGKVVGSPLSVLTLDLKN
                     NMTVSAVSLAGGGALAFAHPGDVLDITMPAPVPVNGTVAVDVTYSGTPIQTGFGSFVF
                     GTRFGDRFAWSLSEPYGARDWWPCKDHPSDKADSVRVTVKVPSQYRVGSQGVLVSETT
                     VGPDKIYDWKSNYPISNYLVSVAVGEYVRHLGTYNRSAPLAGLYGPLSMPLDHLVYDD
                     GSSSLPSGWANVTDVLDVFEGWFGPYPFAGEKYGHAETTFGGGMEHQTMTSLGGTSVG
                     LVAHELGHQWYGDMISPKTWPHLWLNEGFATYSELLYYEARASTYPGQFEGILGARYN
                     SALTAVGKLVLEDTTTVSNMFAFSRVYAKGAVVLHMLRNVIGDVAFKSTMQAYAADPA
                     VQYGVATTADFQRVAEQESGMDLDTFFYQWVTDGFGHPFYRSFSFWQTAPGGGCDVWT
                     TVEQWQTMPYSNIDVFEMPLDIAVTTPSGEERFRFLNDQRHQVFAYHVTEHPLSVAID
                     PDYWILRPFEIFSSIEKTPALLTIVSLAPNPSHDRLRVDFALSRESRVDIEAFDVAGR
                     RVLKRSGVAAGAGPQTETIDVRSLAAGVYFLRLKSIEGVATRKFVVVR"
     gene            3525..>3713
                     /locus_tag="VEC56_00150"
     CDS             3525..>3713
                     /locus_tag="VEC56_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXV12589.1"
                     /translation="MSRIRPLFRDLSDAIRGTEQDYTQGSISRAILLLSVPMMLEMAM
                     ESVFAVVDVYFVSSLGASA"
BASE COUNT          618 a         1203 c         1225 g          667 t
ORIGIN      
        1 gatcgagatc aacccgatca tcatcgactt ggtcaccaac cgctaccgtg actatgtcgg
       61 cgacgccttc cacgacccgc gcgtgcgcct ggtgcaggac gaagggcgca gctgcattgc
      121 gcgcagccgc gagcgcttcg acgtgattca gattaccctg atcgacacct gggcggcgag
      181 tgtgtcgggc gcgtacagcc tctcggagaa ctacctttat acgaccgagg cgttcgcgga
      241 gtacatctcg catttgagcg atggcggact gttgtcgatc acgcgctggt actacgagat
      301 gccgcgcctg gtcgcgctgg cccgcgcggg tctcgaagag accggcgtcg aagcgcccgc
      361 gcaccacgtc ctggtggtgc acgagaagat tcgcgcgctc ttgctcgtga aacactctcc
      421 gttcacgccc gacgaagtgg cgcgcgcgcg cgaatacgcc cacgccatgg ggtggacggt
      481 cgcgcacgac ccggtccagc cggcgcccaa cgcgccctat acgaacctgc tgacggcgcc
      541 cgacccgacc acgatcttca aggacgccga cgttgcgctg cacccggtgc gcgacgacag
      601 tccgttcttc ttccaaatga cgcgctggaa gagcgtcaag ctcgacgcgc tgcggaactt
      661 cgggggagtc ggttttctcg aaccgctcgc gattccggtg gcgcagatcg cgctgttggg
      721 cgcgctcgtc atatcgctcg tgctctccgc ggtgctgatc gcggtgccgc tggtcgtcaa
      781 gaagggccgc gcggttccgc gcgagaaccg ctggacgtgg ctggtgtact tcctgtgcct
      841 ggggctcgcc ttcatcgtgg tcgaagtggt cctgatgcag cgcttcgcgt tgttcttggg
      901 ccacccgacc tactcggtca ccgtcgtctt gttcgcgatt cttctgttct cggggttggg
      961 ggcggcgtgg tcggaccgtc gcggcggaac gccggcggag gtcgcgaagc cggtcatgta
     1021 ctggctcccg atcgcgatcg tcgcactcgc cttcgtggta cccatcgtac tacaaaagtt
     1081 gatcggactg ccccacgcgg cgcgtctcgt gatcgccgtc gcgatgatcg cgccggtcgc
     1141 gttcttgatg ggggttccgt tcccgctcgg catccgcgcc atcgccgcga cgggcggcaa
     1201 gcacgtcccg tgggcttggg ccgcgaacgg ctgtgcgagt gtggtgggct cggtgtgcgc
     1261 ggtgctgggt gccatggtgt ggaacttcag cacgatgctc ttgatcgcgg gcgcgatcta
     1321 cgtgctggcg ctggtcgtgc tggcgcgaat caagacgccg ctgtccgcgt agcctcgact
     1381 tacctcacga caacgaactt gcgcgtcgcc actccctcga tcgacttcag gcgcaggaaa
     1441 tagactcccg ccgccagcga gcgaacgtcg atcgtctcgg tctggggccc ggcgccggcc
     1501 gccacacccg agcgtttgag cacgcgccga cccgccacgt cgaaggcctc gatgtccacg
     1561 cggctctccc ggctcagcgc gaagtcgacg cgcaggcggt cgtgggacgg attgggggcc
     1621 agcgagacga tggtcaggag cgcaggcgtc ttctcgatgc tggagaagat ctcgaagggt
     1681 cgcaggatcc agtagtcggg gtcgatcgcg accgacagcg ggtgctccgt cacgtggtag
     1741 gcgaacactt ggtggcgctg gtcgttcaag aagcgaaagc gctcctctcc cgagggcgtg
     1801 gtcaccgcga tgtcgagcgg catctcgaac acgtcgatgt tcgaatacgg catcgtctgc
     1861 cattgctcga cggtggtcca cacgtcgcac ccgccgcccg gtgcggtctg ccagaagctg
     1921 aacgaccggt agaaggggtg cccgaacccg tccgtcaccc actgatagaa gaacgtgtcg
     1981 aggtccatgc cggattcctg ctccgccacg cgctggaagt cggcggtggt ggccacgccg
     2041 tactgcacgg ccgggtcggc cgcataggcc tgcatcgtgc tcttgaaggc gacgtcgccg
     2101 atcacgttgc gcagcatgtg cagcacgacg gcgcccttcg cgtacacgcg cgagaacgcg
     2161 aacatgttcg agacggtggt ggtgtcttcg aggacgagct ttcccaccgc ggtcagcgcc
     2221 gagttgtagc gcgcccctag gatgccttcg aactgacccg ggtacgtgct cgcacgcgcc
     2281 tcgtaataga gaagctccga ataggtcgcg aatccttcat tgagccacaa gtgcggccag
     2341 gtcttgggcg agatcatatc gccataccat tggtggccca gttcgtgcgc gaccaaaccg
     2401 accgacgtcc cgcccagtga cgtcatggtc tggtgctcca tgccgccgcc gaacgtggtc
     2461 tcggcgtggc cgtacttctc gcccgcgaac ggatacggac cgaaccagcc ttcgaacacg
     2521 tcgagcacgt cggtgacgtt cgcccacccg gagggaagcg acgacgaccc atcgtcgtag
     2581 accaaatgat cgagcggcat cgacagcggg ccgtagagac ccgcgagcgg cgccgagcgg
     2641 ttgtaggtcc cgaggtgacg cacgtattcg cccaccgcga ccgaaaccag gtagttcgag
     2701 atggggtagt tcgacttcca gtcgtagatc ttgtcgggcc cgacggtggt ctcgctcacg
     2761 agcacgccct gcgatcccac gcgatactgc gaggggacct tcaccgtgac gcgcaccgaa
     2821 tcggccttgt cggaggggtg gtccttgcac ggccaccaat cgcgcgcccc gtagggctcg
     2881 gacagactcc acgcgaatcg atcgccgaag cgggtcccga acacgaagct cccgaatccg
     2941 gtctggatgg gagttcccga ataggtgacg tcgaccgcga cggtgccgtt caccggaacc
     3001 ggcgccggca tcgtgatgtc gaggacgtcg cccgggtgcg cgaacgcgag cgcgccgccg
     3061 ccggccagag aaaccgcgct gaccgtcatg ttgttcttga gatcgagggt cagcaccgag
     3121 agcggcgatc ccacgacctt gccttcgaca cggaccacgc cagcgatcga atcgggcgcg
     3181 aagttgaggg tgagcgatag gtgatagtac ccgacgtcga tgttggtgct ggccccggag
     3241 aagggctccc gcacggaccg gaacgagagc gcggccttgg actccgcgca ccggggagca
     3301 ccctggtcgt ccatgacggg tggggccgag ggggtgttac ggacgccgac gagtgctacg
     3361 ccgacgatca gcacgccgag gacgcttctg cgatgcatgg atgctccttc aagtccgcgg
     3421 cgaggttcgg cccgggcgcc gccgcgacct caattataat cgatactgcg accccgacga
     3481 cagtgtggaa aatacctcgt actctctggt agcctcccgc ctctatgtcc cgaattcgtc
     3541 cattgttccg cgacctgtcc gatgccatcc gcggcaccga gcaggactac acgcagggga
     3601 gtatctcacg cgcgatcctc ctcctgtcgg tccccatgat gctcgagatg gcgatggagt
     3661 ccgtcttcgc cgtggtcgac gtgtacttcg tgtcgtcgct gggggcctcg gcg
//
LOCUS       DASPGK010000010         6405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_705906, whole genome shotgun sequence.
ACCESSION   DASPGK010000010 DASPGK010000000
VERSION     DASPGK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705906"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..574
                     /locus_tag="VEC56_00155"
     CDS             131..574
                     /locus_tag="VEC56_00155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV12590.1"
                     /translation="MGKNNVEQMILNIPSDLKYLGAVDAAIQDLAREFSFSPDAINDV
                     STALVEACSNAIEHGNHFAKDKRVVVTLRFNGGDFVATVQDEGRGFDFAKALRDEAGP
                     DPMSERGRGILIMRAFTDDLKFRCDDKGLTVELTKLRGGGGDAGE"
     gene            684..2951
                     /locus_tag="VEC56_00160"
     CDS             684..2951
                     /locus_tag="VEC56_00160"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXV12591.1"
                     /translation="MTGAYSVIVSILYLVCGVVIFLLGLTLLRVGRTSAPTRAAALML
                     FFAGLGPLLSGSGLVLERSLREGAVLYTSMVQNFEYLWEFYFPSLLLFALSFPRENRL
                     LNRYPVIGVLVFLPYIFHLAAMVFGDRMLEQIVKLPAVLTERDLGATIREIDLTRFDT
                     MVAILVRILKRVHRNLFALVNIIYALVALDLLWRSQRGLINPRLARQLRTVSLGVAAS
                     VACYTFAKVSLVLDSALVPTRVNLALLNLSLIASGGAITYAVTRQQFLGVRHIVQRAA
                     LYGAVATVFALIYLVLVRPVTLFFGQYSGASQDVFETGFVVLVVLAFQPALERAEALL
                     ERVLARGRGDLARRFKLLGDAVAAATTFEALEDAVVKGLRDTLDASEVRLAFAYAPQD
                     PLPMALAEIGEPVRRRDLLRVAETRPSRWRRGGKRAAAALEHAARTTVAPDQVPPRAD
                     VLVPVVQERRCVAYVALGEKVYGLTYGAEELGHLSLFSTQIGSAMQNIRLLAESVDRK
                     VFEEELKIARKIQMQMLPGEPPVLDGFELFGVTVPSRQVGGDYYDFVVVDGRWLVVVV
                     ADVSGKGIPASLLTATLQATVRSNADAQTNPVGMMGRLNRLLFRNTSASEFATLFYAV
                     VDLETGRARYANAGHDFPFLLGNGGAHALVESGIVLGCIDEFEYHENHFEIPADGALV
                     VFTDGVTDSESRGGENYGAERLRAVLERHTGETAREMCRRVIEDVRAFGDGENQDDLT
                     LVVLKRRARAEAASR"
     gene            2948..3376
                     /gene="ruvX"
                     /locus_tag="VEC56_00165"
     CDS             2948..3376
                     /gene="ruvX"
                     /locus_tag="VEC56_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXV12592.1"
                     /translation="MSDETGRILAVDPGERRVGLAISDPLGITAQGLPTFDRKGGDVF
                     AHLRALAARYDLVRVVVGRPLSLSGRESDATRRAAEFARAIGDALSLPVDLWDERLSS
                     AEAQRVLAGTRAEKGAVDRVAAVLILQGYLDARRRSDPQP"
     gene            3393..4421
                     /gene="mltG"
                     /locus_tag="VEC56_00170"
     CDS             3393..4421
                     /gene="mltG"
                     /locus_tag="VEC56_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXV12593.1"
                     /translation="MELRRATAIVVVMAIVAGLGFAAWVTKDIQARGPADGERVRVVV
                     SQGAGFNSVVRELEARGLLRRAWTLKLFARATGQDRRVQRGTYEFAKGTPPVDILRAM
                     VRGDVLAFLVTVPEGFNVWQVAGAFDSAGVDSAALFATVTDAGYARQLEIPGATLEGY
                     LFPDTYRVPFGSTPHQVAEHMVARARAAWDDDHERRAREIGLSRHEVITLASIVEAEA
                     RVAEERPTIAAVYHNRLKKGMRLEADPTVAYAMGGFRGRLFYKDLEIDSPYNTYRRAG
                     LPPGPICNPGRASIEAALHPAEGVAALYFVARGDGRHVFSTNLRDHNAAVRDARRNHA
                     SRRSSNGK"
     gene            4473..4658
                     /locus_tag="VEC56_00175"
     CDS             4473..4658
                     /locus_tag="VEC56_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12594.1"
                     /translation="MPANPSQSSTPKASPHFRWKLFAAGLVAITAGYVLLAVNDITIA
                     PVLLVLGYCILVPLAFL"
     gene            4702..4777
                     /locus_tag="VEC56_00180"
     tRNA            4702..4777
                     /locus_tag="VEC56_00180"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4735..4737,aa:Val,seq:tac)
     gene            4962..5726
                     /locus_tag="VEC56_00185"
     CDS             4962..5726
                     /locus_tag="VEC56_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12595.1"
                     /translation="MSSFTTVEGGGLSAGFFAGGRLLINGPSPQPLFGGVGMLKGKFA
                     VLSLAAAALLCLQLASVDTVNSGVVDPCNSSASSAGTYPADITDVVFACPQSDGDAFA
                     PNYTINVSVRDNVNAPVVGVPAADVWLIGCNDLLALCGGSGAISASAATDINGLTTIT
                     GDLSAGGCDTGLRVVVQGVVIGAGVCADPCVAIAVRSADINGSLTVNLADFALFGAGY
                     TSPPKPYTACLDFVTPFAAVNLGDFAKFGSHYNHTC"
     gene            5800..6396
                     /locus_tag="VEC56_00190"
     CDS             5800..6396
                     /locus_tag="VEC56_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12596.1"
                     /translation="MKRALAGVLAIAFVGLMAAGASAQVPYFQVYFDDDSNGSYGETQ
                     ANCGEVNAFQSLYLVASNFNMFVSAVDYQVVLPPALFFISDNLPVVPGGTLALGATPA
                     TISSEGLALSYGLPRSGFSPMLITAMTTVWTGACNCATGPASVLVGPYPGVGKTSPTG
                     IRWGDFQEVGAVGMTSLVCPGPVSTTETTWGGIKALYR"
BASE COUNT         1013 a         2074 c         2117 g         1201 t
ORIGIN      
        1 cgcatcgaga gcatcctcta cgtcaccaag ctcaatctgt tgttcaagtc gttcaactcc
       61 gaggacgaag cggtccggag tttcacggcg gcgcgctgac cggcgtcgtg gcgcggaacc
      121 ggaacggcgt atgggaaaga ataacgtcga gcagatgatc cttaacattc cgagcgacct
      181 gaagtacctc ggggcggtgg acgcggccat ccaggacctg gcgcgcgagt tttctttctc
      241 gcccgacgcg atcaacgacg tcagcaccgc gctggtcgag gcgtgcagca acgccatcga
      301 gcacggcaat cacttcgcca aagacaagcg ggtcgtggtc acgctgcgat tcaacggcgg
      361 cgacttcgtc gcgacggtcc aagacgaagg ccgcggcttc gacttcgcca aggcgctgcg
      421 cgacgaagcg ggccccgacc ccatgagcga gcgcggacgc ggaatcttga tcatgcgagc
      481 cttcaccgac gacctcaagt ttcgatgcga cgacaagggc ttgacggtcg aattgaccaa
      541 gctgcgcggc ggcgggggcg acgccggcga gtgagtccag ccccgcacgc gtcccgcgcg
      601 gcgggggaac cgcccgcgag ccgcgtgggt agaaggggag ttcgcgaccc gcgtccccgc
      661 gatggtcgtc ggagcggctc gagatgaccg gcgcttattc cgtcatcgtt tccatcctct
      721 acctcgtctg cggcgtcgtc attttcctac tggggttgac gctgctccga gtcgggcgca
      781 ccagcgcacc cacccgcgcc gccgccctca tgctcttctt cgcgggcctg ggtccgctct
      841 tgagcggctc cggactcgtc ctcgaacgat ccctgcgcga aggcgccgtg ctctacacca
      901 gcatggtgca gaacttcgag tacctctggg agttctactt tccttcgctc ttgctgttcg
      961 cgctctcgtt cccgcgcgag aaccggttgc tcaatcggta cccggtcatc ggcgtcctgg
     1021 tctttctacc ctacattttt cacttggccg cgatggtctt cggcgaccgc atgctggagc
     1081 aaatcgtcaa gttgcccgcc gtgctgaccg agcgcgacct cggcgcgacc atacgcgaaa
     1141 tcgatctgac gcgcttcgac accatggtcg cgatcctggt gcgcatcttg aaacgcgtgc
     1201 accgcaactt gttcgcgctc gtgaacatca tatacgcgct ggtcgcattg gatctcttgt
     1261 ggcgcagcca gcgcgggctg atcaatccgc gcctggcgcg tcagctgcgg accgtgagcc
     1321 tcggtgtggc ggcgagtgtg gcgtgctaca ccttcgcgaa ggtgtcgctg gtgctggatt
     1381 ccgctctggt gccgacccgc gtcaacctcg cgctcctgaa tctgtcgctg atcgcgagcg
     1441 gcggagccat cacgtacgcg gtgacgcgcc agcaattcct gggcgtacgc cacatcgtcc
     1501 agcgcgcggc actctatggt gcggtcgcca cggtgttcgc gctgatctac ttggtgctgg
     1561 tgcgaccggt aacactcttc ttcggtcaat acagcggcgc gagccaggac gtgttcgaga
     1621 ccggcttcgt ggtgttggtg gtgctcgcgt tccagccggc gctggagcgc gcggaggcgc
     1681 tgctcgagcg cgtgctcgcg cgcggtcgcg gcgacctcgc gcgccgtttc aagctcttgg
     1741 gcgacgcggt ggcggccgcg accacgttcg aagcgctgga agacgccgtg gtcaagggac
     1801 tgcgcgacac actcgacgca tccgaggtcc ggctcgcgtt cgcgtacgca ccgcaggacc
     1861 cgcttccgat ggcgctggcc gagatcggtg agccggttcg acgccgcgac ctcttgcgcg
     1921 tggccgagac gcggccgagc cgatggcggc ggggcggcaa gcgcgccgcg gccgcactgg
     1981 agcacgcggc gcgcaccacg gtcgcgcccg atcaggtgcc gccgcgcgcc gatgtgctcg
     2041 taccggtcgt gcaggagcgc cgatgtgtcg cctacgtggc gttgggcgag aaggtctacg
     2101 gcctcaccta cggcgccgag gagttgggcc acctgtccct gttttcgacc cagatcgggt
     2161 cggccatgca gaacatccga ctcttggccg agagcgtcga tcgcaaggtg ttcgaagaag
     2221 agctcaagat cgcgcgcaag attcagatgc agatgctgcc gggcgaaccg cccgtgctcg
     2281 atggcttcga gctcttcggg gtcaccgtcc ccagccgcca ggtggggggc gattattacg
     2341 acttcgtcgt ggtcgacggg cgctggctgg tggtcgtggt cgcggacgtg tcgggcaagg
     2401 gcatccccgc gtcgctcttg accgcgaccc tgcaagccac cgtgcgttcg aacgccgacg
     2461 cgcaaaccaa tccggtcggc atgatgggac gcctgaatcg gctcctgttc cgaaacacca
     2521 gtgcctcgga gttcgcgacc ttgttctacg cggtggtgga cttggagacg gggcgcgcgc
     2581 gctacgccaa cgccgggcac gatttcccgt tcttgctggg aaacggcggc gcgcacgcgc
     2641 tggtcgagag tggcatcgtg ctggggtgca tcgacgagtt cgagtaccac gagaatcact
     2701 tcgagattcc cgccgacggc gcgctggtgg tgttcaccga cggggtcacc gattcggagt
     2761 cgcgcggggg ggagaactac ggggccgaac gcctgcgcgc ggtcctcgag cgccacaccg
     2821 gcgagacggc gcgcgagatg tgccggcgtg tcatcgaaga cgtgcgcgcc ttcggcgacg
     2881 gcgagaacca ggacgacctc acgctggtgg tcttgaagcg tcgcgcgcgc gcggaggcgg
     2941 cgtcgagatg agcgacgaaa ccggccgcat cctcgccgtg gacccaggcg agcggcgcgt
     3001 cggccttgcg atcagcgacc cactcggcat cacggcccaa ggactgccca ccttcgaccg
     3061 taagggcggc gacgtcttcg cccacctgcg ggcactcgcg gcgcgctacg acctggtgcg
     3121 cgtggtggtg ggaaggccgc tctcgctgtc ggggcgcgag agcgacgcga cgcgtcgcgc
     3181 cgccgagttc gcgcgcgcga tcggcgacgc gctctcgctc ccggtcgacc tgtgggacga
     3241 gcgcctctcg agcgccgagg cccagcgcgt gctggccgga acgcgcgccg aaaaaggcgc
     3301 ggtcgaccgc gtcgcggcgg tcctgatctt gcaaggctat ctcgacgcca ggcgtcgctc
     3361 cgacccgcag ccatgaacca accgaggcgt gcatggaatt gagacgcgca accgcgatcg
     3421 tggtcgtgat ggccatcgtc gccggtctgg gcttcgccgc gtgggtcacc aaggacatcc
     3481 aggcccgcgg ccccgcggac ggcgagcgcg tccgcgtcgt cgtctcacag ggcgccggtt
     3541 tcaattccgt ggtacgcgaa ctggaggcgc gtggtctcct gcgccgcgcg tggacgctca
     3601 agctcttcgc gcgcgccacc ggccaggatc ggcgcgtgca gcgcggtacc tacgagttcg
     3661 cgaaaggcac gccgcccgtc gacatcttgc gcgcgatggt acgcggcgac gtgctcgcgt
     3721 ttctggtgac ggtgccggaa ggattcaacg tctggcaagt cgcgggtgcg ttcgattccg
     3781 ccggcgtcga ttccgccgcc ctcttcgcga ccgtcaccga cgccggctac gcgcgccagt
     3841 tggagatccc gggcgcgacc ctcgagggct atctctttcc cgacacctac cgcgtcccct
     3901 tcggcagcac gccccaccag gtggcggagc acatggtggc gcgcgcgcgc gccgcgtggg
     3961 atgacgatca cgaacgacgc gcgcgcgaaa tcggcctgtc gcgccacgag gtgatcacgc
     4021 tcgcgtccat cgtcgaagcg gaagcgcgcg tggccgaaga acgcccgacc atcgccgccg
     4081 tctatcacaa tcgcctgaag aagggcatgc gcttggaagc ggatcccacc gtcgcctacg
     4141 cgatgggtgg attccgcggg cggttgttct acaaggacct cgagatcgat tctccctaca
     4201 acacgtaccg ccgcgccggt ctccccccgg ggccgatctg taacccgggc cgggcctcca
     4261 tcgaagccgc gctccacccc gccgaaggtg tggcggcact ctacttcgtc gcgcgcggcg
     4321 acgggcgtca cgtgttctcg accaatttgc gcgatcacaa tgcggccgtg cgcgacgccc
     4381 ggcgcaacca cgcgtcgcgg cgttcatcga acgggaagta agcgcgccgg tcgccttgac
     4441 accttcatga cgctgtggtg tagtacacct gcatgccggc caacccgagt caaagttcaa
     4501 cccccaaggc gtcgccgcac tttcggtgga agcttttcgc ggccgggctg gtggccatca
     4561 ccgcgggtta cgttctcctg gcggtcaacg acatcaccat cgcgcccgtc ctgctcgtgc
     4621 tggggtactg catcctggtg ccgctcgcgt ttctctaagc ctggatccgg gggcgacggc
     4681 caccccccgc gccgatccgc cgggcgaata gctcagttgg tagagcgtct gccttacacg
     4741 cagaaggtca caggttcgag ccctgtttcg cccaccatcc gctccacacc taactaatat
     4801 cgaaaaaacg agttatcggg acgcccccgg acccacccgg gaacgggggt cgaagtgggt
     4861 agtcgggggg gccttcttca tgggaacttc gcatacggac gcaaaatagt gcgttgacac
     4921 ggcttttcat tccgtggtag aattttgccc ggtcgagtaa ggtgtcgagt tttacaacgg
     4981 tggagggagg tgggctgtcg gcaggctttt ttgccggcgg caggcttctc atcaatgggc
     5041 catcaccaca accacttttt ggaggtgtag gaatgttgaa aggaaagttc gctgtgctct
     5101 cgcttgctgc cgcagctctg ctttgcctgc agctggcgag cgtcgatacc gtgaacagcg
     5161 gcgtcgtcga tccgtgtaat tcctcggcga gctccgcggg tacgtatcct gcggatatta
     5221 cggacgtcgt gtttgcgtgt ccgcagagcg acggcgatgc atttgctccc aactacacga
     5281 tcaacgtgtc ggttcgggac aatgtcaacg ctccggtcgt aggcgtcccc gcggccgacg
     5341 tttggttgat cggttgtaac gacctgttgg ctctgtgcgg cggcagcggc gcgatcagcg
     5401 cttcggctgc gacggacatc aacgggctga ccacgattac gggcgacttg tcggctggtg
     5461 gttgtgacac cggtctgcgc gtcgttgtcc agggcgtcgt cattggtgcc ggcgtttgcg
     5521 ccgacccgtg cgtcgccatc gcggttcgtt cggccgatat caacggcagc ctcacggtca
     5581 acttggccga tttcgcgctg ttcggtgccg gctacacttc gccgccgaag ccgtacacgg
     5641 cgtgtctcga cttcgtgacg ccgtttgccg ccgtcaacct cggtgacttc gcgaagttcg
     5701 gctcgcacta caatcacacg tgctagcaca gtagcggcgt aagtgactcc taaattgttt
     5761 gcgaccgtga ctttgcagta gaaaaaggag tttgtcgcta tgaagagagc tctagcaggg
     5821 gtgcttgcaa tcgcgttcgt cggcctgatg gccgccggtg cgagcgcgca agtgccttac
     5881 ttccaggtat acttcgacga cgacagtaac ggtagctacg gagagacgca ggcgaactgt
     5941 ggagaggtaa acgctttcca gagcctgtat ctcgtcgcct cgaacttcaa tatgttcgtg
     6001 tcggccgttg actaccaggt cgtccttccg cccgcgttgt tcttcatctc ggataatttg
     6061 ccggtggttc ccggggggac tttggccctc ggtgcgactc cggcgaccat ttcgagtgaa
     6121 ggactggccc tttcgtacgg cctgccgcgg agcggtttct cgcccatgct gattacggcc
     6181 atgacgacgg tctggaccgg cgcgtgcaac tgtgcgaccg gcccggcgtc cgtcctcgtg
     6241 ggtccgtatc ccggcgttgg caagacctct cccacgggta ttcgttgggg tgacttccag
     6301 gaggtcggcg cggtcggtat gacgtcgctg gtctgccctg gcccggtctc caccaccgag
     6361 acgacgtggg gcggaatcaa ggcgctgtac cggtagtagt ttgaa
//
LOCUS       DASPGK010000011         9990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1057807, whole genome shotgun sequence.
ACCESSION   DASPGK010000011 DASPGK010000000
VERSION     DASPGK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057807"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VEC56_00195"
     CDS             complement(<1..656)
                     /locus_tag="VEC56_00195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXV12597.1"
                     /translation="MNVVLEGIRVCASIAIWLLIAPLVPGVINKVKAWITGRKGPPVL
                     QLYFDLARLRRKGIVLSTAVSPGHVVGPAVAFVSLVGASLLLPVGRTGAPIAFEGDAV
                     LFVYLMALARFSMSYAALDTGSSFEGMGAAREVSFAVLAEIVVMTALLVLSVKTGSVS
                     LTKMLAPAAGPGTALLAAGLYMVLLAENCRVPFDDPNTHLELTMIHEVMVLDHSGPPL
                     A"
     gene            complement(653..2599)
                     /locus_tag="VEC56_00200"
     CDS             complement(653..2599)
                     /locus_tag="VEC56_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXV12598.1"
                     /translation="MTPGLFLLFAGTCTALGLFVYRARRVWLITTLVGAAAAFIASGW
                     VLVAGEDWDWQLAVTLAGEGLHLRLDGISAFFLALLAIVGGACSLYAHGYWTDEHHPA
                     SAPSGRAWLSAVLVSMALVLLSTNGLHFLVAWEAFSIGVYFLITLERDKREVRSSGWL
                     FLAASRAGTLCLVVFFVGLATRTGSWELGPMRDRPELAPWFWLALCGFGVKAGMYPLH
                     IWLPSAHGNAPSHVSALLSGVSIKMGIYGLVRFSGWLPTPEAAGWVIVALGAASAVLG
                     VAFALGQHDLKRLLAYHSVENIGIILMGLGFAMMAAAHGGGMWGQLALAGSLLHVWNH
                     GLFKALLFLGAGSVLHATGTREMSRLGGLWRAMPWTAGMFALGAVAIAGLPPLNGFIS
                     EWLLYLGLFGDVSSNGPLSWVAAPAAILLCMTGALALACFVKACGVVFLGLPRSEDAA
                     AAHESSWLMRAPMLLLSLACVIIGLAPALLWPLLARATSTWRPAWTEGVVPVPLASLG
                     GFNIGLVVVSVLVAVGLRRRIARNGIERAVTWDCGYVRPTARMQYTAGSFAAIITAWF
                     ALFLRPRRHARLSRTQFPAHASFEEHTPETVLAYVVEPGSRLVLRVVGAARRLQHGRI
                     QAYVLYVLIGVVALALLSVFGGSR"
     gene            complement(2657..3214)
                     /locus_tag="VEC56_00205"
     CDS             complement(2657..3214)
                     /locus_tag="VEC56_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12599.1"
                     /translation="MAAPRGKAGATRARSKTSARAKAPAPQRPKKPAKPSRARASVTP
                     LLRVTGIGGMFFKSDDPKQLMEWYRKHLGIEPDSYGGWSFKWRELRRTQRIGYTVWSP
                     FEKATDYFDPSEQPYMFNFRVADLKSLLTQLRREGVHVIDKVEEFPFGKFGWIIDPEG
                     RKIELWEPPTSDDPFGSGKKAKPNE"
     gene            complement(3216..3995)
                     /locus_tag="VEC56_00210"
     CDS             complement(3216..3995)
                     /locus_tag="VEC56_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HXV12600.1"
                     /translation="MIRIGKIPYLNCVLFFHGLENEPGVRLEPLVPRALSSAAQEGEV
                     DAGPVPLVTAWEIEDRFSPLGDFCISTIDRSRSILFFSKRPFDQLDGAHIGVTNETST
                     SVRLLRALLTHAWRVRPARLTRVDWPTNDAFLLIGDEALIHRRGVKDYPYVADLGEVW
                     HKWTGLPFVFARWMVRTTLPDADKAQLVTLLDRSIAEGWSHFERVVAPRAHDLHMTTE
                     EMREYLQGFRFRMTAAEHQATGKFREFDEAARRAENAPAAS"
     gene            complement(4011..4751)
                     /locus_tag="VEC56_00215"
     CDS             complement(4011..4751)
                     /locus_tag="VEC56_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12601.1"
                     /translation="MKRKLFIAAAVLLVVIVGIAVYLYNSIDSIVESAIEKHGSRVLG
                     TKVSVGSVDISLKSGRGTIRDVSVENPDGFSSGEMFKLGEITVDIDVSSLNRDPITID
                     EVTISAPTVHVEMNEKAQSNVGVVKAAADKYQSSSPSQAKKQDGGFEKRFIITKFVFE
                     EGTVDLDATAMGVEAVDLELPPVRLSNVGGSNGDTPDGIGKTVSRAFLGAVTSVVTNE
                     VKKRATDKAKQEATDAAKKKLGELLNTN"
     gene            complement(4753..5952)
                     /gene="mqnE"
                     /locus_tag="VEC56_00220"
     CDS             complement(4753..5952)
                     /gene="mqnE"
                     /locus_tag="VEC56_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HXV12602.1"
                     /translation="MLHATDGITFTDRSLVPIWEKVRAGERLSAAAGLRLFESADLTA
                     VGRMADHVARAKNGERVHFVINTHINPTNVCVLNCKFCDYVHSRNDVGAWEMTVEEIL
                     AHITPEMTEVHIVGGHHPDWTFEYHENYIRAIRDAHPHIQIKAFTAAEIDYFAKRWKL
                     SVDEILDRLIAAGLNSMPGGGAEIFSKRVAKELRYSGKAQSDRWLEIHAAAHARGIPS
                     NATMLYGHIETYAERVEHLRLLREQQDRSGGFLAFIPLEYQISAERIVARHASALEDL
                     KTIAVSRLMLDNFPHVKAYWIMLQEDTAGIALNFGADDVDGTISKERIAHAADARSPV
                     GVTRKRLVRLIRDAGKTPVERDALYNIVKVYEDADVAAVLADEYRPVQRSRESPRAGS
                     PLAKNVI"
     gene            6065..6964
                     /locus_tag="VEC56_00225"
     CDS             6065..6964
                     /locus_tag="VEC56_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXV12603.1"
                     /translation="MTARGLVHLLIVYIVWGSTYLGIRIAVRDGSGFPAFAMAGSRVF
                     VAGLILLAWGALAKRKMRPTRQDVEMFAVCGLLLWLGGNGLVTWAEKRAESGYAALLV
                     GSLPLWTTTLESFIDRRRPTLRLIGALLIGMSGIAILNYPTLRHGSNADMLAAGALLL
                     AVMTWGIGSVMQKRRPITLTGEVSSGYQLLFGSFFLIAASLISGEPRPHPTTEAWWAW
                     GYLVLFGSVIAFTSFVKALQLLPVHVVATYAYVNPVIAVLLGWLILAERITPWTIAGS
                     LLVLLGVAGVFHEQRRRNAAKSS"
     gene            7009..8118
                     /locus_tag="VEC56_00230"
     CDS             7009..8118
                     /locus_tag="VEC56_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12604.1"
                     /translation="MHQRWLPTVFLAVLVASSCDNDETSLSVDPPAPTTEELIIADCY
                     VVRDALEAFAAENGGDYPRQSNEPSDAGNLLVTFLPGGTRLTNRYTGLATAPTFGNPQ
                     WPGEIGVVPFGDGYSENGCTCDPTGYRIVARGRYDELVRIENVAAIDPRTITAIDSVL
                     ANLETALATAHEYARRNGGVYAADVGADALPNGDVFTDLLPGGMLMTNPYWGGQDSPV
                     DGSAAGPGQIGYQPQDRNGDGWWDGFIIDALGPDGATVIALRTRDSLEDEDVRSAFIH
                     MRDAVEDFASQNAGEYPRDLDTDETPSGDTLRDLLFNSLNPYTGAPAYRDGLATSRGE
                     VGYVGLEYNGIVVGYVINAVGLFDEELERFEVLTN"
     gene            complement(8115..8603)
                     /locus_tag="VEC56_00235"
     CDS             complement(8115..8603)
                     /locus_tag="VEC56_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXV12605.1"
                     /translation="MTHLDSLDAFSLQFWIPVGVSIVCGAIVGFERQLRGKPLDVRTA
                     VLVCLGTNVYIRLGVVISGEHVDTMRILGQVVTGIGFLGAGVIFNRGAHVQGLTTAAV
                     VWTLAAIGAAIGFDRYSAAIALSLVVFLVLNALEWFENRVEMMRRGAHAHDDREHRER
                     RL"
     gene            complement(8622..9224)
                     /gene="msrA"
                     /locus_tag="VEC56_00240"
     CDS             complement(8622..9224)
                     /gene="msrA"
                     /locus_tag="VEC56_00240"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXV12606.1"
                     /translation="MKTTMIALIAAIAVAIATVSHADEKKKTAIATFAGGCFWCMEPP
                     YDDIKGVKRTTSGYTGGTTKNPSYEEVSDGKTGHAEAVEIEYDPAQVTYQQLLDVFWR
                     NIDPTVTNRQFVDRGTQYRPEIFYHDEEQKRLAEASKKDVEKFFGKVEVQITPAGPFY
                     AAEEYHQDFYMTNPEHYNAYKNGSGRERRLKELWGDKAKK"
     gene            complement(9367..>9990)
                     /locus_tag="VEC56_00245"
     CDS             complement(9367..>9990)
                     /locus_tag="VEC56_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12607.1"
                     /translation="FLYHTDSPLRRKGRMTAHQKARRAYLETSLGRPDPRAVEAGVRD
                     LLEPLLFHKVTSIRSDDHRAYPRAIEGLGGSIAHRITSSKERRDRRNPLWEVNLLDLM
                     IRHSTAAHKRETIAWAKRRQASIEKLAIFQVWRNYIKRRHEKGARGTPAMRLRLATRP
                     WRIRDLLARRLYVERTALSSRWEEYYRRRVVTRALAVNRTHELRYAY"
BASE COUNT         1745 a         3307 c         3149 g         1789 t
ORIGIN      
        1 gccaggggcg gtccgctgtg gtcgagcacc atgacttcgt gaatcatcgt cagctcgagg
       61 tgagtattcg gatcgtcgaa gggcacgcgg caattctcgg ccaggagtac catgtagagc
      121 cccgccgcaa gcagcgccgt acccggcccc gccgccggag cgagcatctt cgtcagggac
      181 acgctgcccg tcttgacgct gaggaccagc aacgcggtca tgacgacgat ctccgcgagc
      241 accgcgaagc tcacctcgcg cgctgcgccc atcccttcga atgacgatcc ggtgtccagt
      301 gcagcgtatg acatcgagaa gcgcgcgagc gccatcaaat agacgaacag cacggcgtcg
      361 ccttcgaagg cgatgggcgc gccggttcgt ccgactggca ggagcagaga cgccccgacg
      421 agggacacga acgccaccgc aggccccacc acgtggccgg gggacacggc ggtgctcaac
      481 acgattccct ttcgccgcaa gcgcgcgagg tcgaagtaga gttggaggac cggcggcccc
      541 tttcgccccg tgatccacgc cttcaccttg ttgatgacac ccggcaccag cggggcgatc
      601 aacaaccaga tcgcgatgct cgcgcacaca cggatccctt cgagcacgac gttcatcggg
      661 atcctccgaa gaccgagagt aaggccaagg ccacgacacc gatcagcaca tagaggacgt
      721 aggcctggat ccgtccgtgt tgaaggcgtc gcgccgcgcc tacgacacgc aggacaagtc
      781 ggctcccggg ttcgaccacg taggccagaa cggtctcggg cgtgtgctct tcgaagcttg
      841 cgtgggccgg aaactgcgtc ctcgataacc gtgcgtgccg gcgcgggcga agaaacaacg
      901 caaaccacgc ggtgatgatc gcggcgaagg aaccggcggt gtactgcatg cgcgcggtcg
      961 gtcggacgta cccgcaatcc caggtgaccg cgcgctcgat tccgttgcgc gcgatgcgac
     1021 gccgaaggcc caccgccacc agcaccgaga ccacgaccag gccgatgttg aaaccaccca
     1081 gagaggcgag tggaacgggg acgactccct cggtccaggc cggcctccac gtcgatgtcg
     1141 cgcgcgccag caacggccac agtagcgccg gcgcgaggcc aatgatcacg cacgccaggc
     1201 tcagcaacag catgggtgcg cgcatgagcc agctgctctc gtgcgccgcg gcggcgtcct
     1261 cgctgcgtgg cagtccgagg aagaccacgc cgcacgcttt cacgaagcac gccagcgcga
     1321 gcgctcccgt catgcaaaga aggatcgccg ccggcgccgc cacccatgac agaggaccat
     1381 tcgacgacac gtcgccgaag agaccgagat acaggagcca ttcgcttatg aaaccgttca
     1441 gcggcggaag ccctgcgatg gccaccgccc ccagcgcaaa catgcccgcg gtccacggca
     1501 tcgcacgcca cagcccgccg agccggctca tttcacgagt acccgtggcg tgcagcaccg
     1561 acccggcgcc gagaaatagc agcgctttga acagaccgtg attccacacg tgcaagagac
     1621 ttcccgccag cgcgagctgt ccccacattc cgccgccgtg ggcggcggcc atcatcgcga
     1681 aaccgagccc catgaggatg attccgatgt tctctacgct gtggtaagcc aacagccgct
     1741 tgaggtcgtg ctggccgagc gcgaacgcca cgcccagcac ggcactggcc gcacccagcg
     1801 cgacgatgac ccagccggcc gcttcaggcg tcggcaacca cccgctgaat ctcaccaatc
     1861 cgtagattcc catcttgatg gagacgccgg agagaagggc cgaaacatga cttggcgcat
     1921 tgccgtgcgc ggatggcagc caaatgtgca gcggatacat cccggctttg acgccgaagc
     1981 cgcacagcgc gagccagaac cacggcgcga gctcgggacg atctcgcatc ggtcccagtt
     2041 cccagcttcc cgtgcgggtc gccaggccaa caaagaagac cactaagcac aacgtccccg
     2101 cgcgcgatgc cgcgaggaag agccaccccg acgaacggac ctcgcgcttg tcacgttcga
     2161 gcgtgatgag gaagtacacg ccgatggaga aggcttccca ggcgacgaga aaatgcagcc
     2221 cgttggtgga gagcagcacc aaagccatgc tgaccaggac ggcgctcaac caggcacggc
     2281 ccgagggcgc cgaagcagga tggtgctcgt ccgtccagta cccgtgcgcg tacaggctgc
     2341 acgcgccccc gacgatcgcg agcagcgcga gaaagaaagc gctgatgccg tcgagacgaa
     2401 ggtggagtcc ctctcccgcc aatgtcaccg cgagttgcca gtcccaatcc tcgccggcga
     2461 cgagtaccca ccccgatgcg atgaatgcgg cagccgcccc aaccagcgta gtgatcagcc
     2521 acacccgccg cgcgcgatag acgaacaagc cgagagcggt gcacgtgccc gcaaaaagaa
     2581 gaaacaggcc tggtgtcatt cccgggttcc gttcatggga tattcgttcg ctcggcggcg
     2641 cgccgcgcag ctgggcctat tcgttcggct tggccttctt cccgctgccg aacggatcgt
     2701 cggaggtggg cggctcccac aactcgatct tgcggccctc ggggtcgatg atccagccga
     2761 acttcccgaa tgggaattct tcgaccttgt cgatcacgtg cacgccttcg cgccgcaatt
     2821 gcgtgagcaa cgacttcaag tcggccaccc gaaagttgaa catgtacggc tgctcgctgg
     2881 ggtcgaagta gtcggtggcc ttctcgaacg gactccaaac ggtgtagccg atgcgctgcg
     2941 tgcgccggag ttcgcgccac ttgaacgacc acccgccgta cgaatcgggc tcgatcccga
     3001 ggtgcttgcg gtaccactcc atgagctgct tggggtcgtc ggacttgaag aacatgccgc
     3061 cgatgccggt gacgcgcagg agcggcgtca cactcgcgcg ggcgcgcgac ggcttcgcgg
     3121 gctttttggg gcgctgggga gcgggcgcct tggcgcgcgc ggaagtctta ctgcgcgctc
     3181 tcgtcgcgcc cgcctttcct cgcggcgcgg ccatgctatg aggccgcggg cgcgttttcc
     3241 gcccggcgcg cggcctcgtc gaactcgcgg aacttcccgg tcgcctggtg ctccgccgcc
     3301 gtcatgcgga agcgaaagcc ctgcaggtac tcgcgcattt cctcggtggt catgtggagg
     3361 tcgtgcgcgc gcggtgccac cacgcgctcg aagtgcgacc acccctccgc gatcgagcga
     3421 tcgagcagtg tcacgagctg cgccttgtcg gcatcgggca gcgtcgtgcg aaccatccag
     3481 cgcgcgaaca cgaacgggag tccggtccac ttgtgccaca cctcgcccaa gtcggcgacg
     3541 tacgggtagt ccttcacacc gcgacgatga atgagcgctt cgtcgccgat aagaagaaac
     3601 gcgtcgttgg tgggccagtc gacgcgcgtc aagcgcgcgg ggcgcacccg ccacgcgtgc
     3661 gtgagcagcg cgcgcaacag gcgcaccgac gtcgaggttt cgttggtgac gccgatgtgc
     3721 gcgccgtcga gttggtcgaa gggccgcttc gaaaagaaca ggatgctgcg cgagcgatcg
     3781 atggtggaga tgcaaaagtc gcccagcgga ctgaagcgat cctcgatctc ccacgcggtc
     3841 accagcggga ccgggcccgc atcgacctcg ccctcttgcg cggcactcga cagcgcgcgc
     3901 gggaccagcg gttccaaccg tacgccgggc tcgttctcga gcccgtgaaa gaacaggacg
     3961 cagttgagat acggaatctt gccgatgcga atcatggttg cgctctctat ctaattggtg
     4021 ttgaggagct ctccgagctt tttcttggcg gcgtcggtcg cttcttgttt ggccttgtcc
     4081 gtcgcgcgct tcttgacctc gttcgtcacc acgctggtca ccgcgcccag gaacgcgcgc
     4141 gatacggtct tgccgatccc gtcgggcgtg tcgccgttgg acccgccgac gttgctcaag
     4201 cgcaccggcg gcagttcgag atccaccgct tcgacaccca tcgcggtcgc gtcgagatcg
     4261 accgtgcctt cttcgaacac gaacttcgtg atgatgaagc gcttctcgaa tccgccgtcc
     4321 tgctttttgg cttgcgacgg cgacgaactc tggtacttgt ccgcggcggc cttgacgacc
     4381 cccacgttgc tctgcgcctt ctcgttcatc tcgacgtgga ctgtgggcgc cgagatggtc
     4441 acttcgtcga tcgtgatggg gtcgcggttg agcgaactca cgtcgatgtc gaccgtgatc
     4501 tcgcccagct tgaacatctc gcccgacgag aagccgtccg gattctccac cgagacgtcg
     4561 cggatggtcc cgcgtcccga cttgagcgag atgtcgaccg agcccacgct caccttggtg
     4621 cccagcacgc gcgagccgtg cttctcgatg gccgactcca cgatggaatc gatcgagttg
     4681 tagagataca ccgcgatgcc gacgatgacg accagcagaa cggcggcggc gatgaaaagc
     4741 ttgcgtttca cgtcagatca cattcttcgc caagggcgaa ccggcgcgcg gagactcccg
     4801 gctccgttgc acgggacggt actcgtccgc gaggaccgcg gccacgtcgg cgtcctcgta
     4861 caccttgacg atgttataga gcgcgtcgcg ctcgaccggt gttttgcccg cgtcgcggat
     4921 cagtcgcacc aggcgcttgc gcgtcacccc gaccgggctg cgcgcgtcgg ccgcgtgcgc
     4981 gatgcgctcc ttcgaaatgg ttccatccac atcgtcggcg ccgaaattga gcgcgatgcc
     5041 cgcggtgtct tcttgcagca tgatccagta cgccttgacg tggggaaaat tatccagcat
     5101 caagcgcgat accgcgatcg tcttcaagtc ttccagcgcc gaggcgtggc gcgccacgat
     5161 cctctccgcg ctgatttgat actcgagcgg gatgaaggcg aggaagccgc cgcttcgatc
     5221 ctgctgctcg cgcagcaggc gcaggtgctc cacccgctcc gcgtaggtct cgatgtggcc
     5281 gtagagcatg gtcgcattgg acgggatgcc gcgcgcgtgc gcggcggcgt gaatttcgag
     5341 ccagcgatcg gactgcgcct tgccactgta gcgcagctcc ttggcgaccc gcttcgagaa
     5401 gatctccgca cccccgcccg gcatcgagtt cagtcccgcc gcgatcagtc gatccaaaat
     5461 ctcgtcgacc gaaagcttcc agcgctttgc gaagtagtcg atctcggccg cggtgaaggc
     5521 cttgatctgg atgtgcgggt gcgcgtcgcg gatggcacgg atgtagttct cgtggtactc
     5581 gaaggtccag tcggggtggt gaccgccgac gatgtggacc tcggtcatct cgggggtgat
     5641 gtgcgcgagg atctcttcga ccgtcatctc ccacgctccc acgtcgttgc ggctgtgcac
     5701 gtagtcgcag aacttgcagt tcagcacaca gacgttggtg gggttgatat gggtattgat
     5761 gacgaaatgg acgcgctccc cgttcttcgc gcgcgcaacg tggtcggcca tgcggccgac
     5821 ggcggtcagg tcggcggatt cgaagaggcg caggccggcg gcggcagaca gtcgctcccc
     5881 ggcgcggact ttctcccaga tcgggaccaa gctgcggtcg gtgaaggtga ttccgtcggt
     5941 ggcgtggagc atggcgacgc gctgcagtat agagcgaacg gtatttcttc acaaggcgct
     6001 ctcaaggccc tagacaagcc catgggcgta tgcttacgtt gcgtctttcc ggagggcccc
     6061 ttccgtgacc gcccgcggcc tcgtccatct tctgatcgtc tacattgtct gggggagtac
     6121 ctacctcggc atccgaattg ccgtccgcga cggctccggc ttccccgcct tcgccatggc
     6181 cggctcacgc gtcttcgtcg ccgggttgat cctgctcgcg tggggcgctc tggccaagcg
     6241 caagatgcgt ccgacgcgcc aggacgtcga gatgttcgcc gtgtgcgggc tgctcttgtg
     6301 gctcggcggc aacgggctgg tgacgtgggc cgagaaacgc gccgagtccg gttacgccgc
     6361 gctcctcgtg ggctcgcttc ccctctggac cacgacgctc gagtcgttca tcgatcgtcg
     6421 ccggccgacc ctccgcctga tcggcgcgct cctgatcggg atgtccggca tcgcgatctt
     6481 gaactatccc acgcttcggc acggctccaa cgcggacatg ctggccgccg gcgccctgct
     6541 gttggcggtc atgacctggg gcatcggatc cgtcatgcag aagcgacgcc ccatcacgct
     6601 caccggtgaa gtgagttccg gctatcagtt gttattcggc tcgttcttcc tcatcgcggc
     6661 gtctttgatc tccggtgagc cccgcccgca cccgaccacc gaggcgtggt gggcgtgggg
     6721 ctacctcgtc ctcttcggct ccgtgatcgc gttcacgtcg ttcgtgaagg cgcttcagct
     6781 gctgcccgtg cacgtcgtcg cgacctatgc ctacgtcaat cccgtgatcg ccgtgctgct
     6841 cggctggctg attctcgccg agcgaatcac gccctggacg atcgcgggat ccctcttggt
     6901 cctcctcggt gtcgcgggcg tcttccacga gcagcggcgc cgcaacgccg ccaagtcctc
     6961 gtagcgcttc tggcacgatt gatgccgtgg gcatacactg gtggtcgtat gcaccagcga
     7021 tggcttccca cagttttcct cgcggttctc gttgcttcga gttgcgacaa cgacgagacg
     7081 tcgctgagcg tcgatccgcc cgctcccacg actgaagagt tgatcatcgc ggactgttac
     7141 gtcgttcgcg atgcgctgga ggcgttcgcg gcggagaatg gcggcgacta cccccggcaa
     7201 tcgaacgagc catccgacgc aggcaatcta ctcgtcacgt tcctccccgg cggcacgcgg
     7261 ctcacgaatc ggtacaccgg actcgcgacc gcgcccacgt tcggtaaccc tcagtggccg
     7321 ggcgaaatcg gcgtcgtccc gttcggcgac ggctactcgg aaaacgggtg cacgtgcgat
     7381 ccgacgggtt atcgcatcgt cgcgcgagga cgctacgatg aattggttcg catcgagaac
     7441 gtcgcggcga tcgaccctcg caccatcacg gccatcgact cggtcttggc caacctcgag
     7501 accgccctgg cgaccgcgca cgagtacgca cgtcgtaacg gcggggtgta cgcggccgac
     7561 gtcggtgccg acgcgttgcc gaatggagac gtgttcacag atctgcttcc cggtggaatg
     7621 ctgatgacga acccgtactg gggaggccaa gattcccccg tcgacggctc cgctgcgggc
     7681 cccgggcaga ttggatacca accccaggat cgcaatggcg acgggtggtg ggatgggttc
     7741 atcatcgacg ctctcggccc cgacggcgct accgtaatcg cgctgcgaac ccgcgactcg
     7801 ctcgaggacg aagacgtgcg atccgcgttc atccacatgc gcgacgccgt cgaagacttc
     7861 gcctcgcaga acgccggcga gtatccgcgc gatctcgaca ccgatgaaac gccttcgggc
     7921 gataccctgc gcgaccttct cttcaatagc ctcaacccgt acaccggcgc cccggcctac
     7981 cgcgacggcc tcgcgacctc gcgcggcgag gtaggctatg tcggattgga atacaacggg
     8041 atcgtggtgg gctacgtgat caacgcggtc ggtctcttcg acgaagagct cgagcgcttc
     8101 gaggtcctga cgaactagag tctgcgttcc cggtgttccc ggtcgtcgtg cgcgtgcgcg
     8161 ccgcgacgca tcatctccac gcggttctcg aaccactcca gggcgttcaa caccaagaac
     8221 acgaccagcg agagcgcgat cgccgcggaa tagcgatcga agccgatcgc ggccccgatc
     8281 gcggccagcg tccacacgac cgccgcggtc gtgagtccct gcacgtgcgc gccgcggttg
     8341 aaaatgacac ccgcgccgag gaagccgatt cccgtcacca cttgtccgag gatccgcatg
     8401 gtgtccacgt gctcgccgga gatgacgacc ccaaggcgaa tgtagacgtt ggtgccgagg
     8461 cacacgagga ccgcggtgcg aacgtcgagc ggcttgccgc gcaactggcg ctcgaagccg
     8521 acgatggcgc cgcacacgat cgacacgccg accgggatcc aaaattggag cgagaacgcg
     8581 tctagtgaat cgagatgggt catggtcgag gcgtcgacgc gctatttctt cgccttgtcg
     8641 ccccacagct ccttcaagcg tcgttcgcgg ccgctgccgt tcttgtacgc attgtaatgc
     8701 tcggggttgg tcatgtagaa gtcctgatga tactcctcgg ccgcatagaa cggcccggcc
     8761 ggcgtgatct ggacctcgac cttgccgaag aacttctcca cgtccttctt cgacgcctcc
     8821 gccagccgct tctgctcttc gtcgtggtag aagatctcgg ggcggtactg tgtcccccga
     8881 tcgacgaact ggcggttggt gacggtcggg tcgatgttgc gccagaacac gtcgagcagc
     8941 tgctggtagg tcacctgcgc ggggtcgtac tcgatctcga ccgcttcggc gtgaccggtc
     9001 ttcccgtccg atacttcctc gtaggagggg ttcttggtgg taccgccggt gtagcccgac
     9061 gtggttcgct tgacaccctt gatgtcgtcg taggggggct ccatgcacca gaagcacccg
     9121 ccggcgaagg tggcgatggc ggtctttttc ttctcgtccg cgtgggaaac cgtcgcgatc
     9181 gcaaccgcga tcgcagcgat aagagcaatc atcgtggtct tcattagcgt ctccttggca
     9241 gtctacgtcc aaccccagtt acgagggaac gggagttttt ggatggcccc caaagaaaaa
     9301 ggttgtggtc gtgagattgt ccctcggcta catcgaaggc cgtcttgagt gaacgatcca
     9361 cgtcggttag tacgcgtacc gcagctcgtg ggtccggttg accgccaggg cacgcgtgac
     9421 cacgcgccgg cgataatact cctcccaacg actcgatagt gccgttctct cgacatacag
     9481 ccttctcgcg agcagatctc ggatcctcca cggtcgagtc gcgagtctca accgcatggc
     9541 cggggtcccc ctcgctccct tctcgtgtcg tcgcttgatg tagtttctcc acacctggaa
     9601 gatcgcgagc ttctcgatcg aggcctgacg ccgcttcgcc cacgcaatcg tctcccgctt
     9661 gtgggcggcc gtgctgtgac ggatcatcag atcgagcaga ttgacctccc agagcggatt
     9721 gcgccgatcc cgccgctcct tcgaggacgt gatccgatgg gcgatgctgc caccaaggcc
     9781 ttcgatcgcc cggggataag cgcggtggtc gtcgcttcgg atcgacgtta ccttgtggaa
     9841 gagaaggggc tcgagcaggt cgcgcacccc ggcctccacc gcccgcggat ctgggcgccc
     9901 cagggaggtc tcgagataag cgcgacgagc cttctggtgc gcggtcatgc gccccttgcg
     9961 gcgaagcggg ctgtcggtgt gatagaggaa
//
LOCUS       DASPGK010000012         4788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_238368, whole genome shotgun sequence.
ACCESSION   DASPGK010000012 DASPGK010000000
VERSION     DASPGK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238368"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="fusA"
                     /locus_tag="VEC56_00250"
     CDS             <1..285
                     /gene="fusA"
                     /locus_tag="VEC56_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807327.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV12608.1"
                     /translation="NLQVKVPDEYMGDVMGDLSGRRGKIQGTENQGRFAVINAQVPMA
                     ELYKYSTHLRSMTQGRGSHSREFSHYEEVPREIAEKIIAEHKAQVAAEHK"
     gene            341..1084
                     /locus_tag="VEC56_00255"
     CDS             341..1084
                     /locus_tag="VEC56_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXV12609.1"
                     /translation="MAGHSKWKQIKHKKAKTDAQRSKVFTKVLREITIAARQGGSDPA
                     GNPRLRLAIQTAKENNMPNDTIMRAAKKGAGELEGQALEEFWYEGYGPAGVALIIDVV
                     SDNRHRTAGEVRHVLTKHNGSLGENGSVSWNFETKGVITVDKKRVDEETLMAAALDAG
                     AADVSDEGEVFEVYTDPAELSTVAELLKKASVPCEAAEVQKVPKTVVKLSGDDARKVL
                     NLVEALEDLDDVQRVSANFDIPDEILTQG"
     gene            1118..1606
                     /gene="ruvC"
                     /locus_tag="VEC56_00260"
     CDS             1118..1606
                     /gene="ruvC"
                     /locus_tag="VEC56_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008489614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXV12610.1"
                     /translation="MYRILGVDPGSNCTGYGLVSGNGDAVSYVSSGTIAPSRSQSRYE
                     RLREIYAGIERVIRELEPTHFAIEDVFYSKNPRSTLVLGEARGAAILAATLAGIPVYE
                     YSAREVKQSVTGNGAADKSQVSYMLVKILGLGNPPERADESDALAIAMCHAFKRRDWE
                     GA"
     gene            1603..2196
                     /gene="ruvA"
                     /locus_tag="VEC56_00265"
     CDS             1603..2196
                     /gene="ruvA"
                     /locus_tag="VEC56_00265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXV12611.1"
                     /translation="MISRVRGRVVSAHPRCVVEVGGVGFEVAIPEKDREALSPTDAEV
                     VFHTHLVVREDRLSLFGFLHRDDRELFLKLIEVSGVGPKLALAVLSLHPAARVVTAIK
                     KGDAAFLSKIPGIGKKTAERLGIELGDKLDDLTSTDEPEAPVSGGVREEVLLALTSLG
                     MTRSAAEAVLDRMEWHPEAEATVEDVVREALKYASRG"
     gene            2198..3226
                     /gene="ruvB"
                     /locus_tag="VEC56_00270"
     CDS             2198..3226
                     /gene="ruvB"
                     /locus_tag="VEC56_00270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXV12612.1"
                     /translation="MTERLTNPAIAADDAKSDALLRPRKLEEFVGQTELKENLRVFID
                     AARQRGEALDHVLLYGPPGLGKTTLAHIVANELEVPIKTTSGPAFQNAAELLGVLSHI
                     EKNQVVFIDEIHRLPRILEEHLYQAMDSLKCELIVDKGPNARHYTLNIEAFTLIGATT
                     RAGMITAPLRDRFGVVMRLDFYTPAELCTIIKRSAGILEIEISDDGADEIARRSRGTP
                     RIANRLLKRTRDFAQVQGKNTIDREAADFALGRLRVDARGLDEMDRRILTSIVQKFGG
                     GPVGIQSLAVAVSEEQETLEDVYEPFLIQQGFLQRTPRGRVATPLALTHLGLTATPGG
                     ADAETRLL"
     gene            3239..4684
                     /locus_tag="VEC56_00275"
     CDS             3239..4684
                     /locus_tag="VEC56_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12613.1"
                     /translation="MRVRNERLLWLLVALAGAWGVWFGTAEGVGVSRDSITYLRGAEH
                     IGHGDGYVGVGDTGRFGPVDHWPPLYSMTIALPVAAGLSVTQAARLVNALALFALLIV
                     TALLVRRLAPDAALAPLIAMIAVLVAPTILRNHLMAWSESIFLPLVLTGFLKVHDYLA
                     RDRVGDLAAAATCFGLAAVDRYSGVAFIAALTVVLALRDVAAGGRRRFVRPLTFVVVA
                     GIPTAVWVVRNMLVASRAHDRPFSPHMPSMNHVRALLDSLSLWLLPQPVPFRARVLAL
                     LVVTTLAVLLARRHRARPGSFWTVTGLCAAAYLGFVLVTISFFDFDVNYDTRMMSPIL
                     VLVVVYLATVGRRAFARPMAAPAKLLVVLFGAALVLLAGYSVTLVRAGHDEPAQFASD
                     RWTHARAWELVASVPSDVPVYASSPYVTAYFVDREVLPLPETPPGGAGFLLFYPNVDG
                     AGATLDPAARSLLNASAVDSTETVILYRIER"
     gene            4688..>4788
                     /locus_tag="VEC56_00280"
     CDS             4688..>4788
                     /locus_tag="VEC56_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXV12614.1"
                     /translation="MHLDDFSYHLPPALIAQAPATERTASRLLVVSRR"
BASE COUNT          804 a         1597 c         1638 g          749 t
ORIGIN      
        1 aatctgcagg tcaaggtgcc cgacgagtac atgggcgacg tcatgggcga tttgagcggg
       61 cgccgcggca agatccaggg caccgagaac caggggcgct tcgccgtgat caacgcgcaa
      121 gtgccgatgg cggagctcta caagtactcg acgcacctgc gctccatgac gcaggggcgc
      181 ggctcgcaca gccgcgagtt ctcgcactac gaagaagtcc cgcgcgaaat cgcggagaag
      241 atcatcgccg agcacaaggc gcaggtcgcc gcggagcaca agtaggggcc gcagtgggct
      301 gttgtgcgac aggacccgcg gcgcacacta gggagtcacg atggcagggc attccaagtg
      361 gaagcagatc aagcacaaga aggcgaagac cgacgcccag cgcagcaagg tcttcaccaa
      421 ggtgctgcgc gagatcacca tcgcggcgcg ccagggcggt agcgacccgg ccggcaaccc
      481 gcgcttgcgt ctggccatcc agaccgccaa ggaaaacaac atgcccaacg acaccatcat
      541 gcgggcggcc aagaagggcg ccggcgagct ggaagggcag gcgctggagg agttctggta
      601 cgaaggctac ggcccggccg gggtcgccct catcatcgac gtcgtcagcg acaaccgcca
      661 tcgcaccgcc ggcgaggtcc gccacgtgct caccaagcac aacggcagcc tgggcgaaaa
      721 cggcagcgtg tcgtggaatt tcgagaccaa aggcgtgatt accgtcgaca agaagcgcgt
      781 cgacgaggaa actctgatgg cggcggccct cgacgcggga gccgccgacg tcagcgacga
      841 gggcgaggtg ttcgaggtct acaccgaccc cgccgagctg tcgaccgtcg ccgagttgtt
      901 gaagaaggcc agcgtgccgt gcgaggcggc cgaggtccaa aaggtgccga agaccgtcgt
      961 caagttgagc ggcgacgacg cgcgcaaggt cttgaacctg gtcgaagcgc tcgaggactt
     1021 ggacgacgtc cagcgcgtga gtgcgaactt cgacattccg gacgagatac tgacgcaggg
     1081 ctagcgggcg cgcgcacggt gcgcgccggg aggggcgatg taccggatac tcggcgtgga
     1141 ccccgggagc aactgcaccg gctacggatt ggtctcgggc aacggcgacg cggtctcgta
     1201 cgtctcctcg gggaccatcg cgccctcgcg gtcgcaatcg cgctacgaac gcctgcgcga
     1261 gatctacgcg ggcatcgaac gcgtgatccg cgagctcgag cccacccact tcgccatcga
     1321 agacgtcttc tacagcaaga atccgcgcag cacgctggtg ctgggcgaag cgcgcggcgc
     1381 cgccattctc gccgccacgc tggccggtat tcccgtgtac gaatactccg cgcgcgaggt
     1441 gaagcagtcg gtgaccggca acggggccgc cgacaagtcg caagtgagct acatgctggt
     1501 caagatactc ggcctcggca atccacccga gcgcgcggac gaatccgacg cgctcgctat
     1561 cgcgatgtgt cacgccttca agcgtcgcga ctgggagggc gcgtgatctc gcgcgtacgc
     1621 ggccgtgtgg tgagcgcgca cccgcgctgc gtggtcgaag tgggcggcgt gggcttcgag
     1681 gtcgcgattc ccgaaaaaga ccgcgaggcg ctttcgccca ccgacgccga ggtcgtgttc
     1741 cacacccacc tggtggtgcg cgaggaccgc ttgagcctgt tcggtttcct gcaccgcgat
     1801 gaccgcgagc tgtttctcaa attgatcgaa gtctccggtg tcgggcccaa gctcgcgctg
     1861 gcggtgctct cgctgcaccc ggcggcgcgc gtggtcaccg ccatcaagaa gggcgacgcc
     1921 gcgtttctct ccaagattcc ggggatcggg aagaagacgg cggagcgtct ggggatcgaa
     1981 ctgggagaca agctcgatga cctgacgagc accgacgagc cggaagctcc ggtgtcgggc
     2041 ggggtgcgcg aagaagtcct gctcgcgctc acgtccttgg gaatgacgcg gagcgcggcg
     2101 gaggcggtac tggatcgcat ggagtggcac ccggaggcgg aggccacggt ggaagacgtg
     2161 gtgcgcgaag cgctcaagta cgcgagccga ggatgacatg accgaacgac tgaccaaccc
     2221 cgccatcgcg gccgacgacg ccaagagcga cgcgctccta cgcccgcgca agctcgagga
     2281 gttcgtcggg cagaccgagc tcaaggagaa cctgcgcgtc ttcatagatg ccgcgcgcca
     2341 gcgcggcgag gcgctcgatc acgtgctgct gtatggcccg ccgggactgg gcaagacgac
     2401 actcgcacac atagtggcaa acgagctcga agtgccgatc aagaccacgt cggggccggc
     2461 cttccaaaac gcggccgagc tgctcggcgt gttgagccac atcgaaaaga accaagtggt
     2521 attcatcgac gagatccatc gcctgccgcg catcctggag gagcacctct accaggcgat
     2581 ggactccttg aagtgcgagt tgatcgtcga caagggaccc aacgcgcgcc actacacgct
     2641 caacatcgag gccttcacgc tgatcggggc caccacgcgc gcgggcatga tcacggcccc
     2701 cttgcgcgac cgcttcggcg tcgtgatgcg gctggacttc tacacgccgg ccgagttgtg
     2761 caccatcatc aagcgctcgg cgggcatcct cgagatcgag atctccgacg acggcgccga
     2821 cgagatcgcg cgccgctcgc gggggacacc gcgcatcgcc aaccggctct tgaagcgcac
     2881 gcgcgacttc gcgcaggtgc aggggaagaa taccatcgac cgcgaggcgg ccgacttcgc
     2941 gctggggcgc ctgcgtgtcg acgcgcgcgg cctggacgag atggaccggc gcattctcac
     3001 ctccatcgtg cagaagttcg gcggcggccc ggttggcatc cagtcgctcg cggtcgcggt
     3061 cagcgaagag caagaaaccc tcgaagacgt ctacgaaccc ttcctcatcc agcaaggatt
     3121 cctgcaacgc acgccgcgcg ggcgcgtggc cacccctctc gcgttgaccc acctcgggct
     3181 cacggcgacg ccaggcgggg cggacgccga gacgcgactg ctctagccgg cgtcccgcat
     3241 gcgcgtgcgc aacgaacgcc tcctgtggtt gctcgtggca ctcgcgggcg cgtggggcgt
     3301 ttggttcggc accgcggaag gcgtgggcgt gtcgcgggac tcaatcacgt acctgcgtgg
     3361 cgcagagcac atcgggcatg gagacgggta cgtgggtgtg ggcgacaccg gccgcttcgg
     3421 tcccgtcgac cactggccgc ccctctacag catgacgatc gcgcttccgg tggcggccgg
     3481 gctttccgtc acccaggcgg cccgcctcgt caacgcgctg gcgctgttcg cgctcttgat
     3541 cgtcacggcg ctgctggtgc ggcggctcgc gcccgacgcc gcgctcgctc cgctgatcgc
     3601 gatgatcgcg gtgctggtcg cgcccaccat ccttcgcaac cacctcatgg cctggagcga
     3661 gtccatcttc ctcccgctgg tgttgacggg attcctgaag gtgcacgact acctcgcgcg
     3721 cgaccgggtt ggtgacctcg cggcggcggc cacgtgcttc ggcctcgcgg cggtcgatcg
     3781 ctactcgggg gtcgcattca tcgcggcact cacggtcgtg ctggccctgc gcgacgtcgc
     3841 cgcgggggga cggcggcgat tcgtgagacc gctgacgttc gtggtcgtgg ccggaatccc
     3901 gacggcggtg tgggtcgtgc gcaacatgct cgtggccagc cgagcccacg atcgcccgtt
     3961 ctcaccgcac atgccgtcga tgaaccacgt tcgcgcgctg ctggactcgc tgtcgctgtg
     4021 gctcttgccg cagccggtgc cgtttcgcgc ccgcgtgctg gctctcctgg tcgtgacgac
     4081 gttggccgtc ctgctcgcgc gccgacaccg cgcgcggccc ggctctttct ggacggtgac
     4141 cggactttgc gcggccgcgt atctcggctt cgtcctggtc acgatctcgt tcttcgactt
     4201 cgacgtcaac tacgatacgc gcatgatgtc gcccattctg gtgctggtgg tcgtttacct
     4261 ggcgacggtg gggaggcgcg ccttcgcgcg cccgatggcg gcgccggcca aactcctcgt
     4321 ggtgctgttc ggcgccgccc tggtgctgct cgccggctac tcggtgaccc ttgtgcgcgc
     4381 ggggcacgac gagcccgcgc aattcgcgag cgatcgctgg acgcacgcac gcgcgtggga
     4441 gttggtcgcg tcggtcccga gcgacgtacc ggtgtacgcg tcgtcgccgt acgtgacggc
     4501 gtatttcgtc gaccgcgagg tgctcccgct cccggagacg cccccgggcg gcgccgggtt
     4561 cctgctgttc tacccgaacg tggacggtgc cggtgccacg ctggatccgg ccgcgcgttc
     4621 gctgctcaac gccagcgcgg tcgactcgac ggagaccgtg atcttgtacc gaatcgaacg
     4681 gtagcgcgtg cacctcgacg acttttcata ccatctcccg cccgcgctga tcgcgcaggc
     4741 gcccgcgacc gagcggaccg cgagccggct gctcgtcgtg tcccgtcg
//
LOCUS       DASPGK010000013         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_823729, whole genome shotgun sequence.
ACCESSION   DASPGK010000013 DASPGK010000000
VERSION     DASPGK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823729"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VEC56_00285"
     CDS             <1..740
                     /locus_tag="VEC56_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV12615.1"
                     /translation="AAYRLGDPYRIAIVAHDVADMPVEAFAVAVKSDPRVSDVVLVML
                     TSLGQQGDAERLRSVGFDAYLTKPTRPSQLADVVSAVWSVKRRGAREVFVTRHTIAEA
                     RAQDVAAHPEAGPRARVLVAEDNLVNQRVAVRLLETLGCRVDVAASGIEAIKMLETGA
                     YDLVLMDCQMPEMDGYEATREIRRRESRAGGRTTIVAMTAHALPGDREKCTEAGMDDF
                     VAKPVRRDTLADVIDRHVAETDPPKGR"
     gene            complement(737..2038)
                     /locus_tag="VEC56_00290"
     CDS             complement(737..2038)
                     /locus_tag="VEC56_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV12616.1"
                     /translation="MRTWIVAIAAVLVLAPRTDARTIVHAGRLIDGVSNAPRERVSVV
                     VDEGRIVDVVAGFVNAGAGDEVVDLSDHTVLPGLMDMHTHLAFELSRTSYMDEFVMNP
                     TDVAIRSTHYARLTLMAGFTTVRDLGDDGMTTVALRKAIATGFAVGPRIFTAGKSIAT
                     TGGHADPTSGWCQALAGDPGPREGVVSGVDDARKAVRQRYKDGADLIKITATGGVLSM
                     ASSGDNAQFSDDELAAIVATGKDYGFSVAAHAHGAEGMLRAVNAGVASIEHGTYMNDE
                     IMKAMKKKGTYYVPTILAGVTVAELATKEDYLPAVVRPKAARIGPQIKDTFTKAYKSG
                     VKIAFGTDTGVSPHGQNAREFELMVECGMPAMTAIQAATMEAAKLLRMEDRLGSIEKG
                     KVADLVAVKDDPLANISTLKDIRFVMKDGVVYKRDGAAVAR"
     gene            2155..3417
                     /locus_tag="VEC56_00295"
     CDS             2155..3417
                     /locus_tag="VEC56_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXV12617.1"
                     /translation="MDATLVRFVIALLIGALIGIEREKTQRQSGEESATGLRTFILIA
                     QAGAVAAWLSREAREPLLFAGVGLVCAAFILAGYRAHVRTAPRSFGLTTEFAALVAYL
                     LGGVALFGDPRLAVALAIATSAVLAFRQVLHGAVERIGWGDIFAALKLLIVVFIVLPV
                     VPRRPVDPWGVINPYPMTWLVILIAGLSLLGYVVARWLGPKRGTAVTGLAGGLVSSTA
                     VTLSLSRRSQEADGPGVSATLASGILLSWAVMFVRVIVEAFVVNPTLAVRLVAPMGVM
                     ALVAVGGAAANHRAGVQVPSSAEFLRLKNPFSLSFAIKFALLFAAVAVLVNRAQELVS
                     ERAVYVVAALAGLTDVDAITLSMAREADHASGSIAITAITIAVLANTLVKTGLVWWVG
                     SRALSARVLPTAIVVAAGAAISLLFVAR"
     gene            complement(3414..>3784)
                     /locus_tag="VEC56_00300"
     CDS             complement(3414..>3784)
                     /locus_tag="VEC56_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HXV12618.1"
                     /translation="IRKAVAGFVLPLGATMNMNGTSLFEGVTVLFLAQVFGVDLSLGQ
                     QVLVIMMSVITAVGAAGIPGGSIPFIIIVLGMFGIPPEGIAIVLGVDRILDMCRTVVN
                     VAGDLVTASIIERIEARTTG"
BASE COUNT          571 a         1259 c         1292 g          662 t
ORIGIN      
        1 aggccgcgta ccggctggga gatccgtacc ggatcgcgat cgtcgcgcac gacgtcgccg
       61 acatgccggt ggaagccttc gcggtcgcgg tcaagagcga cccgcgcgtt tccgacgtcg
      121 tgctggtcat gctcacctcg ctcggccagc agggcgacgc ggagcggttg cggtcggtcg
      181 gcttcgacgc ctacctcacc aagcccacgc ggccgtcgca actcgccgac gtggtctcgg
      241 cggtgtggag tgtgaagcgc cgcggcgcgc gcgaggtgtt cgtcacgcgc cacaccattg
      301 cggaggcgcg cgcgcaggac gtggccgcgc accccgaggc gggcccgcgc gcgcgggtgc
      361 tggtggccga ggacaacctc gtcaatcagc gagtcgcggt gcgcttgctg gaaacgctcg
      421 ggtgccgcgt cgacgtcgcg gccagcggga tcgaagccat caagatgctc gagacgggcg
      481 cgtacgacct ggttctcatg gactgccaga tgccggagat ggacggatac gaagccacgc
      541 gcgagattcg ccgccgcgaa tcgcgcgcgg gcggccgcac cacgatcgtg gccatgacgg
      601 cgcacgcgct gcccggcgac cgcgagaagt gcaccgaggc gggcatggac gacttcgtcg
      661 cgaagcccgt gcggcgcgac accctagccg acgtaatcga tcgccacgtg gccgaaacgg
      721 acccgcccaa gggtcgttag cgcgccacgg cggcgccgtc gcgcttgtag accacgccgt
      781 ccttcatcac gaatcggata tccttcagag tcgagatgtt cgcgagcggg tcgtccttca
      841 ccgctaccag gtccgcgacc ttccccttct caatcgaacc cagtcggtcc tccatacgca
      901 atagcttggc ggcttccatg gtggcggcct ggatcgcggt catcgctggc atgccgcact
      961 cgaccatgag ttcgaactcg cgcgcgttct ggccgtgcgg cgaaacaccg gtgtcggtcc
     1021 cgaacgcgat cttgaccccg gacttgtatg ccttcgtgaa ggtgtccttg atttgcgggc
     1081 cgatgcgcgc ggccttcggc cgaaccactg cgggcagata gtcctccttg gtcgcgagct
     1141 cggccaccgt cacaccggcc agaatggtcg gtacgtagta ggtccccttc ttcttcatcg
     1201 ctttcatgat ctcgtcgttc atgtaggtgc cgtgctcgat cgacgccacg ccagcgttga
     1261 ccgcgcgcag catgccttcc gcgccgtgcg cgtgcgcggc cacggaaaac ccgtagtctt
     1321 ttcccgtggc cacgatggcc gcgagctcgt cgtcggagaa ctgcgcgttg tcgccgctgg
     1381 acgccatact caacacgccg ccggtggccg tgatcttgat cagatcggct ccgtccttgt
     1441 aacgttgtct gaccgccttg cgcgcgtcgt cgactccgct cacgacaccc tcacgcggcc
     1501 ccgggtcgcc cgcgagcgcc tggcaccagc cgctggtggg gtcggcgtga ccgcccgtgg
     1561 tcgcgatcga tttgcccgcg gtgaagatgc gcggtcccac cgcgaagccg gtggcgatcg
     1621 ccttgcgcag cgccaccgtc gtcatgccgt cgtcgcccag gtcgcgcacg gtggtgaagc
     1681 ccgccatgag agtcaacctc gcgtagtggg tcgagcggat cgccacgtcg gtgggattca
     1741 tcacgaactc gtccatgtag gacgtgcgcg agagctcgaa ggcgaggtgg gtgtgcatgt
     1801 ccatcaggcc gggcagcacg gtgtggtcgg agaggtcgac gacttcgtcg cccgcaccgg
     1861 cgttcacaaa gccggccacg acgtcgacga tgcgaccctc gtcgaccacg acggaaacgc
     1921 gctcgcgcgg cgcgttcgac acgccgtcga tcaagcggcc ggcgtgcacg atggtacgcg
     1981 cgtcggtgcg cggagccaga acaagcactg ctgcgatggc gacgatccaa gtacgcatga
     2041 agaacccccg ggttggaagt gctcgccggc gcgaccggcg cgcgccaacg agcacggatt
     2101 atacgaagcc gaggaaccgg cacgcaaggc gcgtggatag gttatgatag tcgcgtggac
     2161 gccaccctgg ttcgattcgt catcgcgctt ctgatcggcg ctttgatcgg catcgagcgc
     2221 gagaagacgc aacgccagtc cggcgaggag agtgccaccg gcctgcgcac gttcatcctg
     2281 atcgcgcagg cgggcgcggt ggcggcctgg ttgtcgaggg aagcgcgcga gccgctgctc
     2341 ttcgccggcg tcgggctggt gtgcgccgcg ttcatcctgg ccggttaccg cgcgcacgtg
     2401 cgcaccgcgc cccgctcgtt cgggttgacc accgagttcg cggccttggt ggcgtacttg
     2461 ctgggaggcg tggcgctctt cggcgatccg cggctcgcgg tggctctcgc aatcgcgact
     2521 tccgcggtgc tcgcgttccg gcaagtcttg cacggggcgg tcgaacgcat cggctggggc
     2581 gacatcttcg ccgcgctcaa gctcctgata gtagttttca tcgtgctgcc ggtggtgccg
     2641 cgtcggccgg tcgacccgtg gggcgtgatc aacccctacc cgatgacatg gctcgtgatc
     2701 ttgatcgcgg gtctgtcgct cctgggttac gtggttgcgc gctggctcgg tccgaaacgc
     2761 ggcaccgcgg tgacggggct cgcgggcggg ctggtgtcgt ccaccgccgt gacactctcg
     2821 ctctcgcggc gcagccagga ggcggacggg cccggtgtga gcgcgacgct ggcaagcggc
     2881 atcctgctgt cgtgggcggt gatgttcgtg cgcgtcatcg tcgaggcgtt cgtggtcaac
     2941 ccgacgctcg cggtgcgact ggtggcaccg atgggcgtca tggcgctggt agccgtgggc
     3001 ggcgcggctg caaaccaccg agccggcgtc caggtcccgt cctccgcaga gttcctgcgc
     3061 ctcaagaacc cgttcagctt gtctttcgcg atcaagttcg cgctcctgtt cgcggcggtg
     3121 gcggtgctgg tgaatcgcgc gcaggagctc gtgagcgaac gcgcggtgta cgtcgtggcc
     3181 gcgctcgcgg gcctgaccga tgtcgacgcc attacgcttt cgatggcgcg cgaagccgac
     3241 cacgcctccg gttccatcgc gatcacggcc ataacgatcg cggtgctcgc aaatacgctc
     3301 gtgaagaccg ggttggtgtg gtgggtgggg agccgcgcgt tgtccgctcg cgtgcttccc
     3361 accgcgatcg tggtcgcggc gggtgcagcg atctcgctgc tcttcgtggc gcgttagccc
     3421 gtcgtgcggg cctcgatgcg ctcgatgatc gaggcggtga cgaggtcgcc ggccacgttg
     3481 accacggtgc ggcacatgtc gaggatgcgg tccacgccca gcacgatcgc gattccttcc
     3541 ggcggaattc cgaacattcc caggacgatg atgatgaagg gaatcgaccc tcctggaatg
     3601 cccgccgcgc ccaccgccgt gatcaccgac atcatgatca ccagcacctg ctgacccagc
     3661 gacagatcga ctccgaacac ttgggcgaga aacagcaccg tgacgccctc gaagagcgaa
     3721 gtgccgttca tgttcatggt ggcacccaac ggcagcacga aacccgccac cgctttgcgg
     3781 atac
//
LOCUS       DASPGK010000014         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_238533, whole genome shotgun sequence.
ACCESSION   DASPGK010000014 DASPGK010000000
VERSION     DASPGK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238533"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VEC56_00305"
     CDS             <1..1319
                     /locus_tag="VEC56_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXV12619.1"
                     /translation="RLKINPRRKAHTDMASTPRTHTRTSTPARKHDRRAGGARAGKPT
                     LPRVGEEMWALVYDAERDRWDKTRGFRREKVAKPELDPKDPLDASAVILKVKFTGFCG
                     SDAGIWFRSSFKNMIHDSLKAEGKTTRVIGHEVFGEVAEVGAVAAAHYGLKKGELVAA
                     ESHIVCGRCHQCLIGQRHVCTDERIIGISRDGGFAEYIKLPASVIWRTDVAKIRPEVA
                     VVQEPFGNAVHACTAVDLRGKSVAIFGCGAIGQFTIMIAKALGASRVIGIEPNPINAE
                     LAEKLGADEVVSFKIDAAADERNGWKAYPDVVQKVVRFSGIDGVDVAMEMAGHNSSVN
                     NALQSVRRGGDVVLFGLKSGDFKIQDFQRTIVKGIRMHSVIGRRLFETWELTKNLLES
                     RENGIQDKIWNIMLNRGKDTIVDAEDFDPATFEKKIGKHPKLIIRW"
     gene            1313..1882
                     /locus_tag="VEC56_00310"
     CDS             1313..1882
                     /locus_tag="VEC56_00310"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXV12620.1"
                     /translation="MVTDRDRPLGPAPWSKRVVAFALDYLVILGYLAILLGLGLGARK
                     LFGPWEKSIVAAEPAAMDVFAFVVAILPVILYFAISESSPSRATWGKRISGLAVVGPN
                     DSRLSQPRALVRAAVKFLPWQLAHSCLFRIEGWPLEPMGPSPTVMAGLAVSQALALWY
                     ALAIALAKSHRAPYDWLAGSLVVTTRQSA"
     gene            1942..3657
                     /locus_tag="VEC56_00315"
     CDS             1942..3657
                     /locus_tag="VEC56_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12621.1"
                     /translation="MNPCLRIVLLAAVLGLAQVQFAQADSVAARKLDSALAAVAAEDW
                     AAAAKLYRELAADNPQQGLFWYELGTAEYHLKNYRESIAAYDRAIAVGQFVGTCYFNQ
                     ACCFALLGEQTPAIDRLELAIKAGLRNRETSIREDTDLVSIRDTEEFRKRILPAPPLD
                     ISRVDGWRMDLDYLTKRVAETHYDPWAHISRAEWDQEIARVSRGIPEMKDHEIIVALI
                     QLMVRIGDGHTGLSAPRQGKFAFHMLPVTFFDFKDGLYVKAAHPDYAQLVGKRVVRVG
                     ALSAKDAMARIQTTAQRDNDQGGRWMGPRYLARIEYLDALGISDGLESVVLTVADAKG
                     KETRITVKAIPFLGSSPHDTEVEPSGWIDMAPPTESPPLWRRQPDRYFTLDYLDDSKI
                     AYANFRAVIDSPHETLADFAGRVVPFARDKKARVLVIDVRTNNGGNNNLARPLLHAIA
                     NSEFNEMGRLFVITGRETFSACQNFCNWMDRQTAAIFVGEPTGSRPNFVGEGNEIILP
                     YSGLTANASSRYWQDSYSEDRRVWIAPELEAEMTSDDYRSNRDPALAAILEYVTTRGL
                     AAAKP"
     gene            complement(3659..>3886)
                     /locus_tag="VEC56_00320"
     CDS             complement(3659..>3886)
                     /locus_tag="VEC56_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12622.1"
                     /translation="TLTPEAYVREIVSGRLSDPTLSFQLNRGFKVLGVVANYMRHDPL
                     SLGYAAVIEYLNPEVATEEDYARQRRSPYYA"
BASE COUNT          724 a         1244 c         1272 g          646 t
ORIGIN      
        1 gtcggctgaa gatcaatcct agaagaaagg cacacaccga catggcgagc acaccgcgaa
       61 cgcacactcg aacgtccacg cccgcgcgca aacacgaccg gcgcgccggc ggcgcccgcg
      121 ccggcaaacc caccctcccg cgcgtgggcg aagagatgtg ggccctggtc tacgacgccg
      181 agcgcgaccg ttgggacaag acgcgcggct tccgccgcga gaaagtcgcc aagcccgagc
      241 tcgatcccaa agacccgctg gatgcgagtg cggtcattct caaggtgaaa ttcaccggct
      301 tttgcggctc cgacgccggc atctggttcc gctcgtcgtt caagaacatg atccacgatt
      361 cgctcaaggc cgaaggcaag acgacgcgtg tcatcggcca cgaggtcttc ggcgaagtgg
      421 cggaagtagg ggcggtcgcg gccgcgcact atgggctcaa gaagggcgag ttggtcgcgg
      481 ccgaatcgca catcgtgtgc gggcgctgcc accagtgcct gatcggccag cgccacgtgt
      541 gcaccgacga gcgcatcatc ggcatctcac gcgacggcgg cttcgcggaa tacatcaagc
      601 tccccgcgag tgtcatctgg cgcaccgacg tcgccaagat tcgccccgaa gtggccgtgg
      661 tgcaggagcc gttcggaaac gccgtgcacg cgtgcaccgc cgtcgacctg cgcggcaagt
      721 cggtcgccat cttcgggtgc ggcgccatcg gacagttcac catcatgatc gccaaggcgc
      781 tgggcgcctc gcgcgtgatc ggcatcgagc ccaatcccat caacgccgag ctggcggaga
      841 agttgggcgc cgacgaagtg gtgtcgttca agatcgacgc ggccgccgac gagcgcaacg
      901 gctggaaggc gtaccccgat gtggtacaga aagtggtgcg ctttagcggc atcgacggcg
      961 tcgacgtcgc catggagatg gccggccaca actcgtcggt caacaatgca ctgcaatcgg
     1021 tgcggcgcgg cggcgacgtg gtgctgttcg gcctgaagtc gggcgatttc aagatccagg
     1081 acttccagcg caccatcgtc aagggcatcc gcatgcacag cgtcatcgga cggcgcctgt
     1141 tcgagacctg ggagctgacc aagaatctcc tcgagtcgcg cgagaacggc atccaggaca
     1201 agatctggaa catcatgctc aaccgcggca aggacaccat cgtcgacgcg gaagacttcg
     1261 acccggccac cttcgagaag aagatcggca agcacccgaa gttgattatt aggtggtaac
     1321 cgatcgagat cgacccctgg ggcccgcccc atggtccaag cgcgtggtcg cgttcgcgct
     1381 cgactacctg gtcatcctcg gctacttggc gatcctcctc ggcctcgggt taggcgctcg
     1441 caagttattc ggcccgtggg agaagtcgat agtcgccgcg gaaccggcgg cgatggacgt
     1501 attcgcgttc gtcgtcgcca tccttcccgt catcctctac ttcgcgatca gcgagagctc
     1561 gccgtcgcgt gcgacatggg gaaagcgaat atcgggactc gcggtggtgg gtccgaacga
     1621 tagtcgcttg agtcagccac gtgcgctcgt acgagcggcg gtcaagttcc tgccgtggca
     1681 gctcgcgcac tcgtgtctct ttcgcatcga aggctggccg ctcgagccaa tggggccgtc
     1741 cccgacggtg atggcagggc tcgcggtgtc acaggcgcta gcactgtggt atgccctggc
     1801 gatcgcgctc gcgaagtcgc atcgcgcgcc ctatgactgg ctggccggct cgctggtggt
     1861 cacgacccgc caatccgctt gaaacttccc accccggccc accgtaggtg ctagaaatcc
     1921 agcaccgaaa ggagcccgct cgtgaatcca tgccttcgga tagtcctgct tgccgcggtc
     1981 ctggggctcg cgcaagttca attcgcccag gccgatagtg tcgccgctcg gaagctcgac
     2041 tcggcgctgg ccgccgtcgc ggcggaggac tgggcggccg ccgccaagct ctatcgcgag
     2101 ttggccgccg acaaccccca gcagggcctg ttttggtacg agctcggcac cgccgagtac
     2161 cacctcaaga actaccgcga atcgatcgcg gcgtacgatc gtgccatcgc ggtcggtcag
     2221 ttcgtcggca cctgctactt caaccaggcg tgctgcttcg cgctgctcgg cgagcaaacg
     2281 cccgccatcg accggctcga gcttgcgatc aaggccgggc ttcgcaaccg cgagacatcg
     2341 attcgcgagg ataccgatct ggtgagcatc cgcgacacgg aggagttccg gaaacggatc
     2401 ctgccggcgc cgccgctcga catctcgcgc gtggacggct ggcgcatgga cctcgattat
     2461 ctcaccaagc gcgtcgcgga gacgcactac gatccatggg cgcacatctc gcgcgccgag
     2521 tgggaccagg agatcgcgcg cgtctcgagg ggcatccccg aaatgaagga ccacgagatc
     2581 atcgtcgcgc tgattcagct gatggtgcga atcggagacg gtcacacggg attgagtgcg
     2641 ccgcgacaag gcaagttcgc gttccatatg ctgcccgtga cgttcttcga cttcaaggac
     2701 ggtttgtacg tcaaggcggc ccacccggat tacgcgcaac tagtggggaa gcgcgtggtt
     2761 cgggtgggcg cgctttccgc gaaggatgcc atggcgcgca tccagaccac ggcgcagcgc
     2821 gacaacgacc agggcgggcg ctggatgggg ccgcgctact tggcgcgcat cgagtacctc
     2881 gacgcgctgg ggatctcgga cggactcgag tccgtagtat tgacggtggc cgacgcgaag
     2941 ggaaaggaaa cccggatcac ggtcaaggcg attccgtttc tcgggagcag cccgcacgat
     3001 accgaagtgg aaccgtcggg ttggatcgac atggccccgc ccacggagtc gccgccgctg
     3061 tggcgccgcc agcccgatcg ctacttcacg ttggattatc tcgacgattc gaagatcgcg
     3121 tatgcgaact tccgcgccgt catcgattcg ccgcacgaga cgctggcgga cttcgccggc
     3181 cgggtggtcc cgttcgcaag ggacaagaag gcgcgcgtgc tggtgatcga cgtacggaca
     3241 aacaacggcg gcaacaacaa cttggcgcgg ccgctcctgc acgctatagc aaactcggag
     3301 ttcaacgaga tgggcaggct gttcgtcatc acggggcgcg agaccttcag cgcgtgccag
     3361 aacttctgta actggatgga tcggcagacc gcggccatct tcgtgggcga gccgacgggt
     3421 tcgcgtccca acttcgtcgg cgagggaaac gagattatcc tgccctacag cgggctcacg
     3481 gcgaacgcgt cgtcgcgcta ttggcaggac tcgtactcgg aggacaggcg ggtgtggatc
     3541 gcgcccgagt tggaggccga gatgacctcc gacgactatc gcagcaaccg tgacccggcc
     3601 ctggcggcga tcctggagta cgtgacgacg cgcggcctag cggccgcgaa accgtgagct
     3661 acgcgtagta cggactcctt cgctggcgcg cgtagtcctc ctcggtcgcg acttcggggt
     3721 tcagatactc gatcacggcc gcgtagccca ggctcaacgg gtcgtggcgc atgtagttgg
     3781 cgacgacacc gagcaccttg aagccccggt tgagttgaaa cgacagcgtc gggtcggaga
     3841 gccggcccga cacgatctcg cgcacgtacg cctcgggcgt gagggt
//
LOCUS       DASPGK010000015         5263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1058355, whole genome shotgun sequence.
ACCESSION   DASPGK010000015 DASPGK010000000
VERSION     DASPGK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058355"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VEC56_00325"
     CDS             complement(<1..442)
                     /locus_tag="VEC56_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXV12623.1"
                     /translation="MTEYIQADELQEKPYDRRLMTRLLRYLKPYKKYVGASMVLLVIE
                     SLLELAPPFITKFAIDRYLTPTGDVALDIRYAGLVKVVIVLGVVLLAGFVSSYAHGLM
                     MSVVGQRVMFDMRMQIFKRLQRLDVSFFDRNPVGRLMTRLTNDVE"
     gene            complement(442..2256)
                     /locus_tag="VEC56_00330"
     CDS             complement(442..2256)
                     /locus_tag="VEC56_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV12624.1"
                     /translation="MNAPTSSKEKRRIALRSVVRYLSRYPGRLALGAASLVAADVLVL
                     ANPWILKTTIDALSRGITKRELLTYAGLLIVITAFGGLFRFLMRRIMIGVSRRIEVDM
                     RADFFRHLQSLSPAFYHRHRTGELMALATNDLNAVRTLVGPAVMYSMNTVVIGSLSIT
                     LMAILSWKLTIAALLPMVVLTVAVYYSIRVIHRLFEIVQSKFAGINARAQENLSGIRV
                     VKAYAREPYEIEQFASASEDYVRANLKLFRVQSMLHPLLSTVAGLGVVAILLFGGRLV
                     IEGEITLGTFVAFNGYLTMLIWPVIALGWVMNMTEQGLASMQRMNDVMAEASEITDAG
                     VPAGAAEASAAAEAPARPRDASIRFEDVSFSYGSNGDRGDILKNVSFEVKDGESVAIV
                     GPTGAGKTTVISLILRLYDTTRGRVLVGGRDVREIPLHELRDMVGLVPQDIFLFSESI
                     RDNIGFGVKELPDPVLQEVASTASIYDEVNEFPNRFESMIGERGINLSGGQKQRVAIA
                     RALAKRPRILVLDDALSSVDATTEEKILGSLAGELENRTAILISHRISTARQADRVIV
                     LADGEIVEQGTHDELIARGGLYADMHHKQMLMVALEGA"
     gene            2340..2900
                     /locus_tag="VEC56_00335"
     CDS             2340..2900
                     /locus_tag="VEC56_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXV12625.1"
                     /translation="MSYPSRDEALAVLEEHTQGESLRKHAYAVEAAMRFYAARLGEDA
                     ERWGVVGLLHDFDYERYPSLDDHPFKGAEILRAKGYDADLIDAILSHADHTGVPRDTP
                     VRKTLFAVDELCGFVTAVTLVRPSKKIEEVEPSSVKKKLKDKAFARNVSREDIAAGAE
                     LLGIPLDEHIRNVITAMRGISDELGL"
     gene            2999..4393
                     /locus_tag="VEC56_00340"
     CDS             2999..4393
                     /locus_tag="VEC56_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV12626.1"
                     /translation="MGLALIALAAPVFAQSDGTIDTRAAIEKRIAELEGMIGGDASAE
                     KQAVTLRWLAELYVAAGRIDAAEAAYERILVFYPYDVASSNAFAAFLLDQRKDPERAL
                     EVTRAAIAWANSAPSPPPYLGRTYAIRARAFADAGQCADALRVAEEAERRADEDAAED
                     AVRTRVRCLSETGRHKEAKAALLELIGETGASNPDDQSALVAMLTRDKKRVDASEVGR
                     MTDAAVRDARTRRAQTLAGENAELIELESSGRVRLEATLRPGQGPSAILFVPDLDSRR
                     SAYTPYAQLFALDGITTLTVDLRGQGDSRCDSLPASDRLPPQHAAELPSDVAAAYAYL
                     TERRKIDPSRIAVVAAGGACVVVERAIHEHNLAPVVVYLSPVFPDDDRELASAFSFRP
                     PRPAFALASEEDIYATRSLRAFKATLDPAPVTTKIYQSAGHGVSLLREPAHFADVDAW
                     TKDALASGASESRQ"
     gene            4458..>5263
                     /locus_tag="VEC56_00345"
     CDS             4458..>5263
                     /locus_tag="VEC56_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12627.1"
                     /translation="MPRLVLVVFLVGITFTAALPAGAQDSSGLAGEWKNEALFDATTT
                     REELLDMGRAAVNMNHVTLAEVYYQEVLLRNPEDVDAMCELAALYRRTGRLEYARGLL
                     IRAGTLVPGRDDIAEARNEVEQRLRASLARQADSLMSAAEYESALPRLSLLLTIDPDN
                     ARFYAAKARCLAALGQSDAALSNVDLALTKDPREEYQRLRTEISAQHEARRIEDLESS
                     AKRLLESNEWMRDEAADVLQAILAQDPSNEWAREQFRALSGTPAPAPPVGA"
BASE COUNT          894 a         1817 c         1723 g          829 t
ORIGIN      
        1 tttcgacgtc gttggtgagc cgcgtcatca gtcgccccac cgggttgcgg tcgaagaacg
       61 acacgtcgag ccgctgcagg cgcttgaaga tctgcatgcg catgtcgaac atgacgcgct
      121 ggccgaccac gctcatcatg agaccgtgcg cgtagctcga cacgaaaccc gcgagcagca
      181 ccacacccag cacgatgacg accttcacca ggccggcgta gcggatgtcg agcgcgacgt
      241 ccccggtggg cgtgaggtaa cggtcgatgg cgaacttggt aatgaagggc ggcgccaatt
      301 cgagcagcga ctcgattacc agcagcacca tgctggcgcc gacgtatttc ttgtacggct
      361 ttagatatct caggagccgc gtcatcaggc ggcggtcgta gggcttctcc tgtaattcgt
      421 cggcctggat gtactccgtc atcacgcacc ctccagggcg accatcagca tctgcttgtg
      481 gtgcatgtcg gcgtagagcc cgccgcgagc gatcagctcg tcgtgggtcc cctgctcgac
      541 gatctcgccg tcggcgagga cgatgacgcg gtcggcctga cgcgcggtcg agatgcggtg
      601 cgagatcagg atcgcggtgc ggttctccag ctcgcccgcg agcgacccca gaatcttctc
      661 ctcggtggtg gcgtccaccg acgagagcgc gtcgtcgagg accaagatgc gcggccgctt
      721 cgccagcgcg cgcgcgatgg cgacgcgctg cttctgcccg cccgacaggt tgatgccgcg
      781 ctcgccgatc atcgactcga agcggttcgg aaactcgttg acctcgtcgt agatcgacgc
      841 cgtgctggcg acttcctgca gcaccgggtc gggcagctct ttcacgccga agccgatgtt
      901 gtcgcgaatc gattcggaga acagaaagat gtcctgcggt accaggccca ccatgtcgcg
      961 caattcgtgc agcggaatct cgcgcacgtc gcgcccgccg accagcacgc gcccgcgggt
     1021 ggtgtcgtag aggcgcagga tcagcgatat cacggtggtc ttgccggcgc cggtcggccc
     1081 gacgatggcc accgactcgc cgtccttcac ttcgaaggac acgttcttca ggatgtcgcc
     1141 ccgatcgccg ttggatccgt acgagaacga cacgtcctcg aagcggatcg acgcgtcgcg
     1201 gggccgggcc ggggcttcgg ccgcggcgct cgcctcagcc gcacccgcgg gtacgcccgc
     1261 gtccgtgatc tcggacgctt ccgccatgac gtcgttcatg cgctgcatcg acgccagccc
     1321 ctgctcggtc atgttcatca cccagccgag cgcgatcacg ggccagatca gcatcgtgag
     1381 atacccgttg aacgcgacga aggtgcccag cgtgatctcg ccctcgatca ccaagcgccc
     1441 gccgaagagg agaatcgcga ccacgcccag ccccgccacc gtcgatagga gcgggtgcag
     1501 catgctctgg acgcggaaca acttcaagtt ggcccggacg tagtcttcgc tggccgacgc
     1561 gaattgctcg atctcgtagg gctcgcgcgc gtaggccttg accacccgga tgccggacag
     1621 gttctcctgc gcgcgcgcgt tgatgcccgc gaacttcgac tgcacgatct cgaacagccg
     1681 atggatgacg cgaatgctgt aatagaccgc gaccgtcagc accaccatcg gcaggagggc
     1741 cgcgatggtg agtttccatg agaggatcgc catcaatgtg atcgaaagac tcccgatcac
     1801 gaccgtgttc atcgagtaca tgaccgcggg gcccaccagc gtgcgcacgg cgttcaagtc
     1861 gttggtcgcg agcgccatca gctcgccggt gcgatgccgg tggtagaagg ccgggctcaa
     1921 cgactgcaag tggcgaaaga aatcggcgcg catgtcgact tcgatgcgcc gcgagacgcc
     1981 gatcatgatg cgccgcatga ggaagcggaa cagcccgccg aacgcggtga tcacgatcag
     2041 cagaccggcg taggtcagga gctcgcgctt ggtgatcccg cgcgagaggg catcgatggt
     2101 ggtcttcaga atccacgggt tggcgagcac cagtacgtcg gccgcgacca ggctggcggc
     2161 gcccagcgcc aatcgccccg gataccggct caggtatcgg acgaccgagc gcagcgcgat
     2221 gcggcgtttt tctttggaac tcgtgggcgc gttcatacta tcatcgtgtg gcaactcgac
     2281 agcgtaatat aggcgatcat cctaccaact cgcaaaccgc cgtagtaagg aaagttcctt
     2341 tgtcgtatcc gtcgcgggac gaagcgctgg ccgtgctcga ggagcacacc caaggcgaga
     2401 gtctgcgcaa gcacgcctac gcggtcgagg ccgccatgcg gttctacgcc gcgcgcttgg
     2461 gcgaagacgc ggaacgctgg ggggtggtcg ggctcctcca cgacttcgac tacgagcgct
     2521 atccgtcgct cgacgaccac cccttcaagg gcgcggagat cctgcgcgcc aagggctacg
     2581 acgccgacct catcgacgcc attctctccc acgccgacca caccggggtg ccccgcgaca
     2641 cgccggtgcg caagaccttg ttcgcggtcg atgagctgtg cggcttcgtc accgcggtga
     2701 cactggtgcg gccgtccaag aagatcgagg aagtcgagcc gtcgtcggtg aagaaaaagc
     2761 tcaaggacaa ggccttcgcg cgcaacgtgt cgcgcgaaga catcgccgcg ggagcggagt
     2821 tgctcgggat tccgctcgac gagcacatcc ggaacgtgat caccgcgatg cgcgggatct
     2881 ccgacgagct cgggctatga tgtcgtcggc cgagcgttcc ccggcgggtg gcgcggctgg
     2941 ggcccccgcc gcgccaggcg gcggggtgag aggcgacagg acccgccggg cagcgttcgt
     3001 gggtctcgcg ctcatcgcgc tcgccgcgcc cgttttcgcg caatcggacg gcaccatcga
     3061 cacccgcgcc gcgatcgaga agcgcatcgc ggagttggaa ggaatgatcg gcggcgacgc
     3121 ctcggccgag aagcaggcgg tgacgctgcg ctggctcgcc gagctctacg tggcggccgg
     3181 gcgcatcgat gccgccgaag cggcgtacga gcgcattctc gttttctatc cctacgacgt
     3241 cgcctcgtcg aacgcattcg cggcgttctt gctcgaccag cgcaaggacc ccgagcgcgc
     3301 gctggaagtg acacgcgccg ccatcgcgtg ggccaactcg gcgccctcgc cgcctcccta
     3361 cctcggtcgc acgtacgcga tccgggcccg cgccttcgcc gacgccggtc agtgcgccga
     3421 cgcccttcgg gtcgcggagg aagcggaacg gcgtgccgac gaagacgccg ccgaagacgc
     3481 ggttcgcacg cgcgtgcgct gcttgtcgga gaccgggagg cacaaagaag ccaaggccgc
     3541 gttgctcgag ctgatcggcg agaccggtgc atccaatccg gacgatcaga gcgcgctggt
     3601 cgcgatgctt acgcgcgaca agaagcgcgt cgacgcgagc gaggtcggcc gcatgaccga
     3661 cgcggccgtg cgcgacgcgc gcacgcggcg cgcgcagacc ctcgcgggcg agaacgcgga
     3721 gctaatcgag ctcgagagca gcggccgcgt tcgcctcgag gccaccctgc gccccggcca
     3781 ggggccctcc gcgatcctct tcgttcccga cctggacagc cggcgctcgg cttacacgcc
     3841 gtacgcgcag ctcttcgcgc tcgacggcat cacgaccctc accgtcgact tgcgcggcca
     3901 aggcgacagt cgttgcgact cgctgccggc gtccgaccgg ctgccgccgc agcacgccgc
     3961 cgagctgccg tccgacgtcg cggccgcgta cgcatacctg accgagaggc gcaagatcga
     4021 tccgtcgcgt atcgccgtgg tcgcggccgg gggcgcgtgc gtcgtcgtcg agcgcgccat
     4081 ccacgagcac aacctcgccc cggtcgtcgt gtatctttcc cccgtgtttc ccgacgacga
     4141 tcgcgagctg gcctccgcct tctccttccg tcccccgcga cccgcgttcg cgctcgcgag
     4201 cgaggaagac atctacgcga cgcgttcgct gcgcgcattc aaggcgacgc tggatcccgc
     4261 cccggtgacg acgaagatct accaatcggc gggccacggc gtctccctcc tgcgcgaacc
     4321 cgcgcacttc gccgacgtcg acgcgtggac caaggacgcg ctcgcgtccg gcgcatccga
     4381 atcgcgccag tagggcgccc ccagggtgcg gccgcacctt ccttccctgg tacgaatgtt
     4441 gcgatttgct gccggttatg ccgcgcctcg tcctcgtcgt tttcctggtt ggaatcacct
     4501 tcaccgccgc cctcccggcc ggcgcccagg attcgtccgg cctcgccggc gaatggaaga
     4561 acgaggcact attcgacgca acgacgacgc gcgaggaact cctggacatg ggtcgcgcgg
     4621 ccgtcaacat gaaccacgtc acgctggccg aggtttacta ccaggaggtt ctgctgcgaa
     4681 accccgaaga cgtggacgca atgtgcgaat tggccgcgct ctatcggcgc acgggccggc
     4741 tcgaatacgc gcgcggactg ctgatccggg ccgggaccct ggtacccggg cgcgacgaca
     4801 tcgccgaggc gcgcaacgaa gtcgaacagc ggctccgtgc gtcgctggcg cgccaagccg
     4861 actcgctgat gtcggcggcc gagtacgaat cggcgctgcc gcgcctctcg ctcctcttga
     4921 ccatcgaccc cgacaacgcg cgcttctacg ccgccaaggc gcgctgcctg gccgcactcg
     4981 gccagagcga cgcggccttg tccaacgtcg acctggccct gacgaaggac ccgcgcgaag
     5041 agtatcaacg cctgcgcacg gagatctccg cccagcacga agcgcgacgc atcgaggacc
     5101 tcgagtcgtc ggccaagcgc ctgctcgagt cgaacgagtg gatgcgcgac gaagcggccg
     5161 acgtgctgca ggcgatcctg gcccaggatc cgtccaacga atgggcgcgc gagcagttcc
     5221 gcgcgctttc gggtaccccc gcgccggcgc cgccggtcgg cgc
//
LOCUS       DASPGK010000016         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1292907, whole genome shotgun sequence.
ACCESSION   DASPGK010000016 DASPGK010000000
VERSION     DASPGK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292907"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VEC56_00350"
     CDS             <1..624
                     /locus_tag="VEC56_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12628.1"
                     /translation="IAGTTSHEWSPAFARATAAFAFCSDRDGTPAAWLLSPGAREPAR
                     VSPARAVADLVSLAPSGNRLAWVDLEGRLIFWNLEAGKGMGVFEPRGVISSASWSPDE
                     NVVVVEAFDWGVAHLYLVGASDGRALMLTYSRTGEGMPSWSPDGAEIAAVTARDGTPS
                     VWVLGNLAPYLDRLREAYDVKTLERPEALRSPPPDGLRRVLPARAKK"
     gene            complement(627..1691)
                     /locus_tag="VEC56_00355"
     CDS             complement(627..1691)
                     /locus_tag="VEC56_00355"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXV12629.1"
                     /translation="MSIFLAIDFSALSGTTRALVVAGGIVLGSSLLGLVIERVLVAGL
                     RRLAASTDVKFDDIVVSSLRGVARWGFFLLGLFAALPALPIPVSFSDEITMVGRLTVL
                     VFAFIILGRFASGIAAHYAHRLFPSSVSLTKIVVNAVVMTIGLLIIFQTIGIKITPIL
                     GALGVGGLAVALALQDTLANFFSGLQVMASKQINLGDYVQLDGGQEGFVSDIGWRAAT
                     LKMLNNNVVIVPNTKLAQAIVTNYSLEERDFAVRMTIGVAYDSDLEKVERITIEEARN
                     VVGRLEGTTKNFDPVVRYHTFGDSAILFNVIMRYQDYGAQFLAQHELVKALNKRYRDE
                     GIEIPFPIRTVVMRGGAPPS"
     gene            complement(1725..2345)
                     /locus_tag="VEC56_00360"
     CDS             complement(1725..2345)
                     /locus_tag="VEC56_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12630.1"
                     /translation="MITQRGRPTNTCRTAWAVAAGAFILMLAGCGDEGDPVDPDGRLC
                     DGETGVGLLIEGRADPLEFCVDDPDVSVVLTSQNRYDVQAQVSNGAGVFVVRMVFAVR
                     SFPATLRITEDLAEAVADIDAVWLYYQEIPAGGDPIESFVVDAGTFTLSFVDEDVATG
                     VIRGVVFDMRDFTTGDPAGQREFSEGIFSISTKEPTAAPRVAATAR"
     gene            2375..2929
                     /locus_tag="VEC56_00365"
     CDS             2375..2929
                     /locus_tag="VEC56_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12631.1"
                     /translation="MRVQFLILFLVALGVTACSKRDASQEQAARSDSAVVATNENGPP
                     VVVREAGVRFDPPSTWPQARYRVDAKAGDAAAAEQPGAAHWVAIQYRPDQPGHEEALL
                     CRIVVFSSDAWTRIDAAPHPPVGTLIESFGAWAFVAQLPQSNPYPQDSLDGAQFEAMR
                     LSIREMRARFSVEGDGPSAEAGDL"
     gene            2934..3647
                     /locus_tag="VEC56_00370"
     CDS             2934..3647
                     /locus_tag="VEC56_00370"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXV12632.1"
                     /translation="MRARAWWAAGVCAAMWASSTGAQSEQDMTEQREKMVDRQIERRG
                     IEDERVLKALREVKRHLFVPPEEISEAYEDHPLPIGHGQTISQPYIVALMTESIEPKA
                     TDRVLEIGTGSGYQAAVLSKLVKEVYSIEIVEALGREAAERLKNLGYANVTVRVGDGY
                     KGWPDQAPFDAIVVTAAPPEVPQALVDQLAEGGRMVVPVGTSFQELLLIEKKKGGEVT
                     KHVITAVRFVPMVKGKETE"
     gene            4162..4503
                     /locus_tag="VEC56_00375"
     CDS             4162..4503
                     /locus_tag="VEC56_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12633.1"
                     /translation="MHWEYAPDYGTFPDDYEQGFGSAAPSEAYYAVEKVVDQFGNDVV
                     WIGAASGTQGLIFTSVPWDEFDGAHFCCAFFGIENKETSNTRAPGSISDQCCFSSLEV
                     GRFAYMTRETS"
BASE COUNT          831 a         1380 c         1562 g          764 t
ORIGIN      
        1 atcgccggca ccacgtcgca cgaatggagc cccgcgttcg cgcgcgccac ggccgcgttc
       61 gcgttctgca gcgaccgcga cggaacgccc gcggcgtggt tgctctcacc gggcgcgcgc
      121 gagcccgcgc gcgtctcgcc ggcgcgggcc gtcgccgact tggtgagctt ggctccgtcg
      181 gggaatcgac tggcctgggt cgacctcgag gggcggctca ttttttggaa tctcgaagcc
      241 ggtaaaggca tgggggtgtt cgagccgcgt ggagtgatct cgtcggcgtc gtggagcccg
      301 gacgaaaacg tggtggtcgt ggaggcgttc gactggggcg tggcgcacct ctacttggtc
      361 ggcgcgagcg acgggcgcgc gctcatgctc acgtactcgc gcacgggcga gggcatgccg
      421 tcgtggagtc ccgacggcgc cgagatcgcc gcggtgaccg cgcgcgatgg gacgccgagc
      481 gtgtgggtgt tggggaatct cgcgccgtat cttgatcgcc tgcgcgaggc ctacgacgtg
      541 aagacgctgg agcggccgga ggctctgcgc tcgccgccgc cggacgggct tcggcgcgtg
      601 ctcccggcgc gcgcgaagaa atagtcttag ctcgggggtg cgcccccgcg cattaccacg
      661 gtccgaatcg ggaagggaat ctcgatgcct tcgtcccgat accgcttgtt gagcgccttg
      721 accaactcgt gctgggcgag gaactgggcg ccgtagtcct ggtagcgcat gatcacgttg
      781 aacagaatgg cggagtcccc gaaggtgtgg tagcgcacca ccgggtcgaa gttcttcgtc
      841 gttccctcga gccgccccac cacgttgcgc gcctcttcga tggttatgcg ctctaccttt
      901 tcgaggtcac tgtcgtaggc cacgccgatg gtcatgcgca ccgcgaagtc gcgctcctcg
      961 agcgagtagt tggtgacgat ggcctgcgcg agcttggtat tcggaacgat gaccacgttg
     1021 ttgttgagca ttttcaaagt cgccgcgcgc cagccgatgt cgctgacgaa accctcctgg
     1081 ccgccgtcga gctgcacgta gtcgcccagg ttgatctgct tggaggccat gacctgcagt
     1141 cccgagaaga agttggccag tgtatcctgc agcgcgaggg ccaccgccag gccgcccacg
     1201 cccagcgcgc ccaggatcgg cgtgatcttg atcccgatgg tctggaagat gatcaacaac
     1261 ccgatcgtca tcacgaccgc gttgacgacg atcttggtca ggctgaccga cgacgggaac
     1321 aaccgatgcg catagtgggc cgcgatcccg ctcgcaaaac gaccgaggat gatgaacgca
     1381 aacacgagga cggtcaggcg tccgaccatt gtgatctcgt cgctgaagct cacgggaatg
     1441 ggcagcgcgg ggagtgcggc gaagaggccg agcaggaaga acccccagcg cgccaccccg
     1501 cgcagcgacg atacgacgat gtcgtcgaac ttgacgtcgg tggaagcagc cagtcggcgg
     1561 agtcccgcga ccaagacgcg ctcgatgacg agacccagga ggctcgaacc gagcacgatc
     1621 ccgcccgcca ccacgagcgc gcgggtggtg cccgagagag cggagaaatc gatggcgagg
     1681 aagatactca cgcgcgagag attaccctct cgggcgggcg atcgtcaacg cgcggtcgcc
     1741 gcgacgcgtg gggccgcggt cggctccttg gtggatatgg aaaagatgcc ctccgagaac
     1801 tcgcgctggc ccgcgggatc gccggtcgtg aagtcgcgca tgtcgaacac gacgccccgg
     1861 atcacgccgg tggccacgtc ttcgtcgacg aaggagagcg tgaaggtccc ggcgtcgacg
     1921 acaaaggact cgatggggtc gccgccggcg gggatctctt ggtagtagag ccacaccgcg
     1981 tcgatgtcgg cgacggcttc ggccaggtct tcggtgatgc ggagggtcgc gggaaacgag
     2041 cgcacggcaa ataccatgcg caccacgaac acgcccgctc cgttggagac ctgggcctgg
     2101 acgtcgtagc ggttctgcga ggtcaggacg acggagacgt cggggtcgtc gacgcagaac
     2161 tcgagcgggt cggcgcgtcc ttcgatgagc aggccgacgc cggtctcgcc gtcgcatagg
     2221 cgcccgtccg ggtcgacggg gtcgccttcg tcaccgcaac cggccagcat aagaatgaac
     2281 gccccggcgg cgaccgccca cgcggtacgg caggtgttgg tgggacggcc gcgctgcgtt
     2341 atcatagggg caagataggc gggaccccat aagcatgcga gttcaatttc tcatcctatt
     2401 cctcgtggcg ctcggcgtga cggcgtgctc gaagcgcgac gcgtcgcagg agcaagccgc
     2461 gcgctccgac agcgcggtcg tggcgaccaa tgagaacggc cccccggtcg tcgtgcgcga
     2521 ggcgggcgtg cggttcgacc cgccctcgac gtggccgcag gcgcgctatc gcgtggacgc
     2581 caaggcgggg gacgccgccg cggcggagca gccgggtgcc gcgcactggg tcgcgatcca
     2641 gtaccgcccc gaccaaccgg ggcacgagga agcgctgttg tgccgcatcg tcgtgttctc
     2701 gagcgacgcc tggacgcgca tcgacgccgc accgcacccg cccgtcggca ccctgatcga
     2761 aagtttcggc gcgtgggcgt tcgtggccca gctcccgcag tcgaatccgt acccgcaaga
     2821 ctccctcgac ggcgcgcagt tcgaagcgat gcgcctctcc atccgcgaga tgcgcgcgcg
     2881 cttctcggtc gagggagacg ggcccagcgc cgaagccggc gatctgtgac gccatgcgcg
     2941 cgcgtgcgtg gtgggcggcc ggcgtgtgcg cggcgatgtg ggcgagcagc accggcgcgc
     3001 aaagcgagca ggacatgacc gaacagcgcg agaaaatggt ggatcgccag atcgagcggc
     3061 gcggcatcga ggacgagcgc gttctgaaag cgctgcgcga ggtcaagcgc cacctcttcg
     3121 ttccgccgga agaaatctcc gaagcgtacg aagaccaccc gctccccatc ggtcacggac
     3181 aaaccatctc ccagccctac atcgtcgcct tgatgaccga gtcgatcgag cccaaagcca
     3241 ccgaccgcgt actcgagatc gggaccggct cgggctacca ggccgccgtg ttgtcgaagc
     3301 tcgtcaagga agtctattcg atcgagatcg tcgaggcgct cggacgcgag gccgccgagc
     3361 gcctgaagaa cctcggctac gccaacgtga ccgtgcgcgt cggggacggc tataaaggat
     3421 ggcccgacca ggcgcccttc gacgcgatcg tggtgaccgc cgcgccgccg gaggttccgc
     3481 aggcactcgt cgaccaactg gccgaaggcg gccgcatggt ggtcccggtg ggcacgtcgt
     3541 ttcaggagct actattgata gaaaagaaga agggcggcga ggtgacgaag cacgtcatca
     3601 cggcggtgcg cttcgtaccg atggtgaagg ggaaggagac ggagtgatag gacagtcagc
     3661 cggaaaacct gcggggtgtt ggcgatcgtc gttgcgcagg tcaatcttgc ggcatcggac
     3721 ctttctgcga agggcgtggt tcttcggtcg atagctgtat tgttgacacc gatcacagga
     3781 ctctgatagg gggtaattcg agtggagaac ctcgcccgct acccgggaga agctgcggcg
     3841 cttcagaaac gacttggaag gagtacacta ggctcgttca cacctccaat tttgatgtct
     3901 ctcctggcaa cggcactctc gtcgtcatcc gctatcgcgc agaccggccc tcttcaatcc
     3961 tatgtaacgt acacgggata tcatcagccc ggcagcatag ctgggtacac gggctcgcgg
     4021 gggtatattg aataccgtcc cgcgataggt acagaagcac tggcggcttg gggcggcatc
     4081 gataaccaga agggaggcca gaatggtgac tgccatatcc aggttgggtg gataaggtgg
     4141 ccccaggcca ctgaggggca ggtgcattgg gaatacgctc cggactacgg cacgttccct
     4201 gacgattatg agcagggttt tgggagcgcg gctccgagcg aagcctacta tgctgtcgag
     4261 aaagtagtcg atcagtttgg taatgacgtc gtttggattg gtgccgcgag tggtacgcaa
     4321 ggactgattt ttacatcagt accgtgggac gagtttgacg gcgctcattt ttgctgcgcg
     4381 ttttttggca ttgaaaacaa ggaaaccagc aatacgcggg cccctggatc gatcagcgac
     4441 cagtgttgtt ttagctccct ggaagtgggg cgctttgcgt atatgacacg agaaactagc
     4501 tagtcgggag gaagctgcgt gtgagccacg tcatcct
//
LOCUS       DASPGK010000017         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_239245, whole genome shotgun sequence.
ACCESSION   DASPGK010000017 DASPGK010000000
VERSION     DASPGK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239245"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VEC56_00380"
     CDS             complement(<1..811)
                     /locus_tag="VEC56_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXV12634.1"
                     /translation="MKKVGVVGCGLMGSGIAQMSALNGYATIVVEVNDEILKKGMASI
                     QDWLEKGKKRGKVTDEQIEAVKKNLSSSTQLSALKDCDIIVEAVTENTELKKKVFKQL
                     SDVVRDDAILATNTSSISVTELATVVKNPERMVGMHFFNPVPIMKLVEIIRGLATTDA
                     VMAASREFALSLGKEPITCPDTPAFVVNKLLIPYLLDAVRMVQDGIATPEDVDKAMVH
                     GCGYPMGPITLLDYVGLDTTLHAADVMYAEFRESKYASPVLLRRMVQAGRWG"
     gene            complement(843..1616)
                     /locus_tag="VEC56_00385"
     CDS             complement(843..1616)
                     /locus_tag="VEC56_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXV12635.1"
                     /translation="MIHADRKEECMLKHGFADGVATITIANPPVNVLTVAMLREMAAA
                     IREAERAKVLLVNSEGGVFSAGVDVGEHREPHAAEMISAFRAVFDALMEFEGIAVAAV
                     DGVALGGGCELACLCDVVVASDRAEFGQPESQVGVLPPVASVLWPRAFGYGRAIDLLL
                     TGARVGAREMHDAGLVSRVVPHARFDAAVASTVEQYRTMSRVVASHVKRACRIGAGEV
                     GAALKQVEALYLNSLMKSKDADEGLRAFLEKRAPKWRDQ"
     gene            complement(1613..2797)
                     /locus_tag="VEC56_00390"
     CDS             complement(1613..2797)
                     /locus_tag="VEC56_00390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXV12636.1"
                     /translation="MSSRVAILDAKRTPIGKFLGAFRNQSAVSLGADVARATIASSGV
                     AAGDVDEVWFGHARQAGNGPNPARQVSVRAGISETVPAYTVNAACGSGLKAIALGADS
                     IRLGRASLVVSGGMENMSQVPFLLDRVRTGYRLGHAPLVDDMYRDGFLCPLCDMVMGE
                     TAEVLAEEFKITRAEQDAWAAMSQNRAERAAKAGLFADEIVHVTVGGERGESKIVDRD
                     EHPREGVTAEALAKLAPVFKKGGTVTAGNASGITDGAAAVVLASEQVVKASGRAPLAW
                     VRDFVATGVEPRRMGIGPVPAVRQLLERNDLDLGRIDVIELNEAFAAQVIACERELRF
                     DRERVNRNGGSISLGHPIGCSGTRVVVTLVHEMRRAGAKLGIATLCVSGGMGMAMLVE
                     RA"
     gene            2997..>4476
                     /locus_tag="VEC56_00395"
     CDS             2997..>4476
                     /locus_tag="VEC56_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12637.1"
                     /translation="MIKKTLVNWVDRFSPRRFARIAPLIRQIVFARVAATVLMLPTGV
                     AADAAQAQTMTSPRSSSARISDPTARAIELDLGGVFLTQKDSGAYAFTHAHAGLAAHP
                     LMASMSLDAFYATGNKGVLSQAAFSIGQYYTYLITAHDRDGDRLVESAAPWGGKDARA
                     EDPAYNALLAIDLRALSRANFELRRVLPALYWYDCSRAVGRSVVAGTFDSDASYFFPN
                     DAATNRSVRQWSALAALPVVFDEFIGPNHAEAVVSRHLMRWATDFAARGSDTEVGPTQ
                     RAVERLVGVTVMRTTDHDDSADALRGLRVLSSSTSESISLYATERAMIDRPLRDPDIA
                     LDLFFAVARWSKKFTDPELVRLEKCLPDVRSLSRGFPVSPVNIGEQSVRTTYTALSQL
                     RDNLRASSFWSAEDRVAYPGADATIATSRLLDDVSTMLRRAENALFAQRFGAAGLRVT
                     TQFPKDASVIGEFAVLNWELSTTGRTVEIKTAMAGVFGEALGP"
BASE COUNT          703 a         1601 c         1408 g          764 t
ORIGIN      
        1 ggccccagcg tcccgcctgc accatgcggc gcaggagcac gggcgacgcg tacttggact
       61 cgcggaactc cgcgtacatc acgtcggcgg cgtgcagcgt cgtgtcgagc ccgacgtagt
      121 cgagcagcgt gatcggcccc atgggatacc cgcagccgtg caccatggcc ttgtcgacgt
      181 cttcgggcgt cgcgatgccg tcctggacca tgcgcaccgc gtcgagcagg tacgggatca
      241 agagcttgtt gacgacgaag gcgggcgtgt ccggacacgt gatgggctct ttgcccagcg
      301 agagcgcgaa ctcgcgactc gcggccatga ccgcgtcggt ggtggcgagc ccccggatga
      361 tctcgaccag cttcatgatc ggcaccgggt tgaagaagtg catgccgacc atgcgctcgg
      421 gattcttcac cacggttgcg agttcggtga ccgagatcga ggatgtattc gtggcgagga
      481 tggcgtcgtc gcgcactacg tcggagagct gcttgaacac cttcttcttg agttcggtgt
      541 tctcggtcac ggcctcgacg atgatgtcgc aatccttgag cgcggagagc tgggtcgacg
      601 acgagaggtt cttcttgacc gcctcgatct gctcgtcggt gaccttaccg cgcttcttgc
      661 ccttctcgag ccagtcctgg atgctggcca tgcccttctt caggatctcg tcgttgacct
      721 cgaccacgat ggtcgcatag ccgttcagcg cgctcatctg cgcgatgccc gagcccatca
      781 aaccgcaacc aaccactccg accttcttca tcttgattcc tttctctcga acctcgccgc
      841 ggttactggt ctctccattt cggcgcgcgt ttttcgagaa acgcgcgcag tccttcgtcc
      901 gcgtccttgg acttcatcag cgaattgaga tacaacgcct cgacctgttt gagcgcggcg
      961 cccacttcgc cggcgccgat gcggcaggca cgcttgacgt ggctcgccac cacacggctc
     1021 atggtccggt attgctcgac ggtcgatgcg acggccgcgt cgaagcgcgc gtgcgggacc
     1081 acacggctca ccagcccggc gtcgtgcatt tcgcgcgcgc cgacgcgagc gccggtgagc
     1141 aagaggtcga tggcgcggcc gtatccgaat gcgcgcggcc acaacaccga cgccaccggc
     1201 gggagcacgc ccacctggga ctccggctgc ccgaactcgg cgcgatcgct cgcgaccacg
     1261 acgtcgcaca gacacgcgag ctcgcagccc ccgcccagtg ccacgccgtc taccgcagcc
     1321 accgcgatcc cttcgaactc catgagggcg tcgaatacgg cgcgaaaggc cgagatcatc
     1381 tcggccgcgt ggggctcgcg gtgctcgccg acgtccacgc cggcgctgaa cacgccgccc
     1441 tcggagttga ccagcagcac cttggcgcgc tcggcctcgc ggatggcggc cgccatctcg
     1501 cgcagcatcg cgaccgtcag cacgttgacc ggcgggttgg cgatcgtgat ggtcgcgaca
     1561 ccgtcggcga acccatgctt gagcatgcac tcttcctttc ggtcggcgtg gatcacgcgc
     1621 gctccaccag catcgccatg cccattccgc ccgagacgca caacgtcgcg attcccagct
     1681 tggccccggc gcggcgcatc tcgtgcacga gcgtcaccac gacgcgcgtc cccgagcacc
     1741 cgatgggatg acccagactg atcgaaccgc cgttgcggtt cacgcgctcg cgatcgaagc
     1801 gcagctcgcg ctcgcacgcg atcacctggg ccgcgaaggc ctcgttcaac tcgatgacgt
     1861 cgatccggcc caggtccaga tcgttgcgct cgagcagctg acgcacggcc ggcacggggc
     1921 cgatgcccat gcggcgcggc tcgacaccgg tcgcgacgaa gtcgcggacc cacgcgagcg
     1981 gcgctctccc ggacgccttc accacctgct ccgacgcgag taccaccgcg gccgcaccgt
     2041 cggtgatgcc ggacgcgttg cccgcggtga cggtcccgcc tttcttgaag acgggagcga
     2101 gcttcgccag agcttcggcg gtcacaccct cgcgcgggtg ttcgtcgcgg tccacgatct
     2161 tcgattcgcc gcgctcaccg cccacggtga cgtgaacgat ttcatccgcg aagaggccgg
     2221 ccttcgccgc gcgttccgcg cggttttgac tcatcgccgc ccacgcgtcc tgctcggcgc
     2281 gcgtgatctt gaattcctcc gccagcacct cggcggtctc gcccatcacc atgtcgcaca
     2341 gcgggcacaa gaagccgtcg cggtacatgt cgtcgaccaa gggtgcgtgc cccagccggt
     2401 atcccgtgcg cacgcgatcg aggagaaacg gcacctgcga catgttctcc atgccgccgg
     2461 agacgaccag cgacgcgcgc ccgagtcgaa tcgaatcggc gccgagcgcg atcgccttca
     2521 atcccgaccc gcacgcggcg ttgacggtat acgcgggcac ggtttcggaa atgccggcgc
     2581 gcacgctcac ctggcgcgcg gggttggggc cgttgcccgc ctggcgggcg tggccgaacc
     2641 acacttcatc gacgtcgccc gcggcgactc cggacgacgc gatggtcgct cgcgcgacgt
     2701 cggcgccgag actcaccgcg gactgattcc gaaatgcacc gaggaacttg ccgatggggg
     2761 tccgttttgc atcgagaatg gcgacgcgcg aggacacgat ccccttcgag tcccgttcga
     2821 cgcggcgcgc gcgacacccg ggacctgcag attaacgact gcaatctcaa taagttgctc
     2881 ggtccgtagc ctatccgacg gggtccccat cgtcaagaaa aactagggga gcaggccggg
     2941 gttcggtgcg ctctttgcaa ggcggccggg gggtagtcgt gcgcccgcgt aatcccatga
     3001 ttaaaaagac acttgtaaac tgggtcgacc gcttttcgcc gcgccgtttc gcgcgcattg
     3061 caccgctcat ccgccagatc gtcttcgcgc gggtggcggc gacggtcctc atgctgccca
     3121 ccggggtggc ggccgacgcg gcccaggccc aaacgatgac gtccccgagg tcctcgagcg
     3181 cgcgcatttc ggaccccacc gcgcgtgcca tcgaactcga cctcgggggc gtcttcctaa
     3241 cccagaagga ctcgggggcc tacgcgttca cccacgccca cgccgggctc gcggcgcacc
     3301 cgctgatggc ctcgatgtcg ctcgacgcgt tctacgccac cggcaacaag ggcgtgctga
     3361 gccaggccgc gttctcgatc gggcagtatt acacgtacct catcaccgcg cacgaccgcg
     3421 acggcgaccg tctggtcgaa tcggccgcac cgtggggcgg caaggacgcc cgcgccgagg
     3481 acccggcgta caacgcactg ctcgccatcg acctgcgcgc tctttcccgc gccaacttcg
     3541 aactgcgtcg cgtgctgccc gcgttgtatt ggtacgattg ctcgcgcgcg gtcgggcgtt
     3601 cggtcgtggc aggcaccttc gactcggacg cgagttattt ctttcccaac gacgcggcca
     3661 ccaatcgcag cgtccgccag tggagcgcgc tcgcggccct gcccgtcgtg ttcgacgagt
     3721 tcattggtcc caaccacgcc gaagccgtcg tctcgcgcca cctcatgcgc tgggccaccg
     3781 actttgccgc gcgcggatcg gacaccgagg tcggtccgac ccagcgcgcg gtcgagcggc
     3841 tggtcggcgt caccgtgatg cgcaccaccg accacgacga ctccgccgat gcgctgcgcg
     3901 gcctgcgcgt gctctcgagc tccacgtcgg aatcgatctc gctctatgcc accgagcgcg
     3961 ccatgatcga ccgtccgctc agggatcccg acatcgcgct ggacctcttc ttcgcggtcg
     4021 cccgctggtc gaagaagttc accgacccgg agctcgtgcg cctcgagaag tgcctgcccg
     4081 acgtgcgttc gctctcgcgc ggcttccccg tctcgcccgt gaacatcgga gagcagtcgg
     4141 tgcgcaccac ttacaccgcg ctttcgcagc tgcgcgacaa cctgcgcgcg agctcgttct
     4201 ggtcggccga ggaccgggtc gcctacccgg gtgccgacgc caccatcgcg accagccgac
     4261 ttctcgacga cgtctcgacg atgctgcggc gcgccgagaa cgcgctcttc gcccaacgct
     4321 tcggggccgc cggattgcgc gtcaccactc agttccccaa ggatgcgtcg gtaatcgggg
     4381 agtttgcggt actgaactgg gagttgagca cgaccgggcg aacggtcgag atcaagaccg
     4441 cgatggcggg cgtattcggc gaagccctcg gtcccg
//
LOCUS       DASPGK010000018        11366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1293142, whole genome shotgun sequence.
ACCESSION   DASPGK010000018 DASPGK010000000
VERSION     DASPGK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293142"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEC56_00400"
     CDS             <1..207
                     /locus_tag="VEC56_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12638.1"
                     /translation="IVGLCLEYGAIAQTHQVIDRHLERAGDTLSRFSPSPAKDLLAGV
                     VKDLRAYAVQQVENFAGFAREAAG"
     gene            297..668
                     /locus_tag="VEC56_00405"
     CDS             297..668
                     /locus_tag="VEC56_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12639.1"
                     /translation="MKNLLLVAFVASTLLRPSLVLCMEPTGDVRLERQEAVCCESEHA
                     TADVAFSARTADDCDGCLDISLAMHSLTLKRVATPNPPTMPYFVLPVVPLLATSTTHA
                     RSTETLNGLHSLILSTIVIRC"
     gene            776..2065
                     /locus_tag="VEC56_00410"
     CDS             776..2065
                     /locus_tag="VEC56_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV12640.1"
                     /translation="MVLVVFLLLALSAGAGARAATSSVWDSVPDSAAARPDTLSFSGV
                     LTRIKLGSPALRAIPYRVTAATALVDQAGAWPNPALLAEAENVNGGYSGLDQSEFSLW
                     LAQEFELGGKRAKRVDQAASAVDEAALEASSESFAVYLDAKARYAAVVHAEERIRLAV
                     EAEALVAHLARAAGDRVRAGAALTADAALADAALARTRLAIDTARAERTSARVALSAL
                     WGQSTGFDEPVTSELPFVRSVLPTDSTQAWAARSPSVARLRIASAARRADAALERSLR
                     VPNFTLAAGARRVEADDASTLLFAVGLPLPVWDRRSAATRAAEARGRAAEIEVDAARA
                     SVASALVARITTLAVLQDRLHKTQDIVAPALATALDNMRTAYAIGRVSYSDLLEAQRA
                     LIALYNDANDARLAIVDETIAIERLTGRTLEELMGNE"
     gene            2058..3023
                     /locus_tag="VEC56_00415"
     CDS             2058..3023
                     /locus_tag="VEC56_00415"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV12641.1"
                     /translation="MNKHSRFSVLATACVALVLASVQCSREAATDQHAEDHDADHGHE
                     HDPTSLGKVTMMPDAFEANGVEIARSGPGLVSVEIDLPGEVVLNADRVAHIVPRFPGV
                     VLQVNKALGDPVRSGEVLAVVQSNVSAAAYDVVSMVDGTVIEKHVALGEFVRDDADIF
                     VVADLSTVWVNISVYARFLTDVKRGQKVRITSPGVRATARGTIDYVGPIVGESTRTGV
                     ARMVLPNPERLWQPGLFVTAHITVSEERVPVAVPDGAIQTVEGREVVFVRRMNEFTAR
                     PIVTGRRGADLIEVVEGLAPGEDYVAAGSFIFKAEFGKSEAGHEH"
     gene            3032..6220
                     /locus_tag="VEC56_00420"
     CDS             3032..6220
                     /locus_tag="VEC56_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXV12642.1"
                     /translation="MLERIIGLSIRNRWAVIAVTLAIAALGVYNFGRLPIDAVPDITN
                     VQVQINTEAAGMSPIEVEQQVTARIELAMGGIPGVDYMRSLSRYGLSQLTIVFHDDVD
                     IYFARQLVSERLREASEGLPPGTEQPAMGPIATGLGEIYMWAVEAEPGALRPDGQPFT
                     ATDFREAQDWIVKPQLRTVPGVTDVNSIGGYVKQYHVTPDPAKLVAHGLTFRDILDAL
                     WQNNLNAGAGYIEHVGEQYLVRIPGVVTTLDDVEKILVGVEDGTPVRVRDLAAVGLGQ
                     ELRTGAATENGEEVVLGTVFMLVGANSRTVSKAVGARMEQIQGTLPEGLKVRTVYDRT
                     RLVDATLHTVRENLTYGAVLVVVVLLLLLGNVRAALLVALTIPLAGLFAVTGMVQNRI
                     SGNLMSLGAIDFGIIVDGAVVMVENMVRRFGRRQHELGRLLTREERLVEAFQSAREVA
                     KPTLFGVAIIMIVYLPILTLGGIEGKMFKPMALTVLLALAGALILTFTFVPAGVAIFL
                     RGRVAERESAVVRGTKRLYAPALRHALRFRWPVIGAAALLVAGCLLLGTRMGSEFIPS
                     LDEGDIAMHAMRIPSTSLTQAVAMQHQLEEKIETVPEVERVFAKIGTAEIATDPMPPS
                     VADVFIIMKPRSGWPDPRKSKDQLVREIEELVSHVPGNNYEFTQPIEMRFNELIAGVR
                     SDLAVKIFGDDTDVMLEEARKIADILGDIPGAADVKAEQVTGLPLMTIVVDRDAIARY
                     GLDVADVQDVIEIAIGGKSAGHVWEGDRRFDLVVRLPEEARLDLHALEFLPIPVPAGT
                     GRAGTSAASLDGIRHLEAGYLPLSAVAEIRIAEGPNQISRENGKRRIVVQANVRGRDL
                     GSFVAEAQRRVEAGVDLPAGYWIEWGGQFENLIAARKRLAIVVPLSLLFIFTLLFATF
                     NSVKHALIVFSGVPLALTGGILALWTRGIPFSISAGVGFIALSGIAVLNGLVMVTFIN
                     SLRRDGAALDDAITQGALSRLRPVLMTALVAALGFVPMAIATGSGAEVQRPLATVVIG
                     GIVSSTLLTLVVLPSLYRVFHRTRPIPPRAVS"
     gene            6358..7269
                     /locus_tag="VEC56_00425"
     CDS             6358..7269
                     /locus_tag="VEC56_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXV12643.1"
                     /translation="MTRCAGIALCWILLSLTPTGSFAGGTATANEAASPLIGDDGGLG
                     SDVFDAVGSTALHLGTDFYEQLSYPFVLAGRDRSRFLLGSAGIVALILTDRFTYQPMA
                     HPPFLPDESLVGPARTLSNLGNTRNAIPLVLGFGAAGLITGSHREKQTSIMLAEALVT
                     SGVWTSLLKYASGRERPREMQEAVADWTGPGGAFADDDTRGGHASFPSGHATGIWAAA
                     TVLAHQYPQGRVVPIAAYGTAAAISYSRMVVGAHFLSDVVVGGLIGYGCARQVIGAHE
                     RSEGPSRFHLLYEPSGDEQRVGLAMDF"
     gene            7387..9738
                     /locus_tag="VEC56_00430"
     CDS             7387..9738
                     /locus_tag="VEC56_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXV12644.1"
                     /translation="MAVNHKPDLDHAFRSQSEHFVHWEKLKDCIDQYIDIMLNYRQSG
                     HPGGSRSKVHAMVSLLLSGAMRWDIRHPEKRFGDRFALVAGHTVPLIYATLPALAETL
                     RVKHERTQNPRYFIPKERMVWWEDLSMLRRNKGLPGHAEMEGKTLFVKANTGPSGHGS
                     PPAAGQAFALKRAGAEGVRVFAFEGEGGLTPGAAHETMNSAYGLGLTNFHYVVDWNDF
                     GIDDVAYSHFVYGTPRDWFGAHGWRVSEAKDGSSWESVTRAMFDMVMGDNPERRPSMM
                     FMRTRKGREYLKYDNKSHGTPHPMNSELFWGTKKPFMEKYGVSFAGFGEPAPKEVSEV
                     AAQCRANLEVVAGVIRSDSALVDYVAERLVEIGESVPGDIAGFKLDVSKNPGRDAKIT
                     DFKSYPESMWAKPGEKQPNRAALARWGAWVNAYCRSHYDRPLFIAMSADLAESTNIAG
                     FAKEWEDVKGYGWFDRDKNLDGILLPQGITEFTNSGIATGIASVNFSKTPYEDFNGFY
                     ATCSTYGSFVYLKYGPMRLFSQLAQDSQIKVGKVLWIAGHSGPETAEDSRTHFGIFSP
                     GVTQLFPDGHVIDVHPWEFNEVPVVIAAALKTSAPIIALHLTRPAVEIPDRAKLGMAS
                     HFEAARGAYVIRPYRKDQKKMGVVVVQGTSTTNNVVKILPKLDEAGLNVKIVAAISPQ
                     LFALQDSAYRDSVFSALDRADAMAITNRARRLMNDWVDYGLTGEYWLSSDWDNRWRTG
                     GSVDEVVEEAHLSPRWILEGIRRFAADRAQRLQKQRARIDELA"
     gene            complement(9735..10772)
                     /locus_tag="VEC56_00435"
     CDS             complement(9735..10772)
                     /locus_tag="VEC56_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV12645.1"
                     /translation="MGNPRNRMLVGTWLVVWGILLLLVSNHVLLGWKHLWPCILVVSG
                     VVMLRVLQSRLTFAVIFAAAWMILLGLFLTAFSFGLIEWGRMRSLWPIIPMIIGVSFV
                     VAGARRPPTSAGVVVGSMVILMGTACMLYETGTISARVAQPFLRWWPLVLVLAGFVLM
                     KRDARARDAAPAPERGHAPVGHAFDQGALSTDVENAILRRVRGAKGSRDAAGALVHEL
                     KDRFDRFSWVGIYRLQGELLSLDDAEFVGASPEHRRIPLSEGVCGAAARERETIVVPD
                     VCADDRYLACSPSVKSEIVVPIVDRGHIVGVLDIDSDQLDAFSDDDRRFLESLVAKAA
                     RNIGEEPRAVA"
     gene            10837..11064
                     /locus_tag="VEC56_00440"
     CDS             10837..11064
                     /locus_tag="VEC56_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXV12646.1"
                     /translation="MPQELGRTLIVIGAVLLVVGCALAFADRIPFLGRLPGDVVVKKR
                     NFTLYVPIATGLLLSLILTVLLNLWSHRGGQ"
     gene            11184..>11366
                     /locus_tag="VEC56_00445"
     CDS             11184..>11366
                     /locus_tag="VEC56_00445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV12647.1"
                     /translation="MKQLVAAAALAALIAGAAGAQTTKKELPVVVFETTKGNIEIELY
                     PDKAPVTVQNFLYYVDN"
BASE COUNT         1902 a         3880 c         3722 g         1862 t
ORIGIN      
        1 atcgtgggct tgtgcctgga gtacggggcc atcgcgcaga cccaccaagt catcgaccga
       61 cacctggagc gcgcgggcga cacgctgtcg cgtttctcgc cgtctccggc caaggatctc
      121 ctggccggcg tggtcaagga cctgcgcgcc tacgcggtcc agcaagtcga gaacttcgcc
      181 ggcttcgccc gcgaagcggc gggataagcc cggatgacgg ggcccggctg gcacgtttct
      241 ggctttttgc gcgcgcgccg ggacccaggg acgccggcgg tgtatactcc acgctcatga
      301 aaaatctcct cctcgtggca ttcgtcgctt ccactctgct gcggcccagc ttggtgctgt
      361 gcatggagcc cacgggcgac gtgcgtttgg aacgccagga ggccgtctgt tgcgagtccg
      421 agcacgcgac ggccgacgtc gcgttcagcg cgaggaccgc ggacgattgc gacggatgtc
      481 tcgatatctc cttggcgatg cactcgctga ccctcaagcg cgtcgccacc ccgaatcccc
      541 cgacgatgcc gtacttcgta ctgcccgtcg tgcctttgct cgcgacgagc acgactcatg
      601 cgcgatcgac cgaaactctc aacggcctcc attccctcat tctgtccacc atcgtcatcc
      661 gctgttgatc cccggcccgc gcacgcgggc ttccagtgtt cgttcgtgag cgtctcgcgg
      721 tgtccgcggg cgcagcttcg ttgttattgg tgctctgacg gtggagcgtg gtcgcatggt
      781 tctagtcgtg tttctacttc tcgccttgtc cgcgggtgcc ggcgcacgcg cggcgacgtc
      841 gtcggtgtgg gactcggttc ccgattccgc ggcagcgcgg cccgatacgc tttccttctc
      901 cggggtcctg acgcgaatca agctcggcag ccccgcgctg cgcgcgattc cctaccgcgt
      961 gacggccgcg acggcgttgg tcgaccaagc cggcgcatgg ccgaaccccg ccctgctggc
     1021 cgaggccgag aacgtcaacg gcggctattc ggggctcgac caatccgagt tttcgctgtg
     1081 gctcgcgcag gagttcgagc tgggcggtaa gcgcgcgaag cgagtcgatc aggcggccag
     1141 cgcggtcgac gaggcagcgc tcgaagcctc gtccgaatcc ttcgcggtct atctggacgc
     1201 gaaggcgcgc tatgccgccg tggtacacgc ggaggaacgg atccgtcttg cggtcgaagc
     1261 cgaagccctg gtggcgcacc tcgcgcgcgc ggccggcgac cgcgtgcgag cgggagccgc
     1321 cctgaccgcc gacgcggccc tcgccgacgc cgctctcgcg cgaacgcgcc tcgccatcga
     1381 caccgcccgt gcggagcgaa ccagcgcgcg cgtcgcgctc tcggcgttgt ggggccaatc
     1441 gaccggcttc gacgaaccgg tgacgagcga actgccgttc gtgcgctccg tgctcccgac
     1501 cgattcgacg caggcgtggg ccgcgcgcag cccgtcggtg gcgcggctgc ggatcgcgag
     1561 cgccgcgcgg cgcgccgacg ccgcgctcga acgcagcctg cgggttccca atttcaccct
     1621 cgccgccggc gcacgccggg tcgaagccga cgacgcgtcg accttgctgt tcgcggtggg
     1681 actcccgctt cccgtctggg accggcgatc ggcggcgacg cgcgcggcgg aagcgcgcgg
     1741 gcgggccgcg gagatcgagg tcgacgccgc ccgggcgagt gtggcgagcg ccctcgtcgc
     1801 gcgcatcacc accctcgcgg tgctccagga ccgcctgcac aagacccagg acatcgtggc
     1861 gcccgcgctg gcgaccgcgc tcgacaacat gcgcaccgcc tacgccatag ggcgcgtcag
     1921 ctactccgac ctgctggaag cacagcgcgc gctgatcgcc ctctataacg acgccaacga
     1981 cgcgcgactc gccatcgtcg atgagacgat cgcgatcgag agattgaccg gccgtaccct
     2041 cgaggagctg atgggcaatg aataagcatt cgcgattctc ggtgctggcc accgcgtgcg
     2101 tggcgctcgt gctcgcgagc gtccaatgct cgcgcgaggc cgccaccgac cagcacgcgg
     2161 aggaccacga cgccgaccac ggccacgagc acgacccgac cagcctgggc aaggtaacca
     2221 tgatgcccga cgcgttcgag gccaacggcg tcgagatcgc gcgctccggg cccgggctgg
     2281 tcagcgtcga gatcgacctt ccgggcgagg tcgtgctcaa cgccgaccgc gtggcgcaca
     2341 tcgtgccgcg cttccccggc gtggttctgc aggtgaacaa ggccctcggc gaccccgtgc
     2401 gctcgggcga agtgctggcc gtcgtgcaga gcaacgtcag tgccgcggcc tacgacgtgg
     2461 tatcgatggt ggacggcacc gtcatcgaga agcacgtggc cctcggggag ttcgtgcgcg
     2521 acgacgccga catcttcgtg gtcgcggacc tctccacggt gtgggtcaac atctccgtgt
     2581 acgcgcgctt cctgaccgac gtgaagcgcg gccagaaggt gcgcattacg tcgcccggcg
     2641 tgcgcgcgac cgcgcgcggc accatcgact acgtgggtcc gatcgtgggc gagtctaccc
     2701 gaaccggtgt cgcgcgcatg gtcctcccca atcccgagcg actgtggcaa ccggggttgt
     2761 tcgtcaccgc gcacatcacg gtctccgagg aacgcgttcc ggtagccgtc cccgacggcg
     2821 cgatccaaac cgtcgaaggg cgcgaagtcg tcttcgtccg gcgcatgaac gagttcaccg
     2881 cgcgcccgat cgtcaccgga cggcgcggcg ccgacttgat cgaagtcgtg gaagggctcg
     2941 cgccgggcga ggactacgtg gccgccggga gcttcatctt caaggccgaa ttcggcaagt
     3001 ccgaagccgg ccacgagcac taggagtcgg catgctggaa cgaatcatcg gactctcgat
     3061 tcgcaaccgg tgggcggtga tcgcggtgac actcgcgatc gcggcgctcg gcgtttacaa
     3121 cttcggccgg ctccccatcg acgcggtgcc cgacatcacg aacgtccagg tgcaaatcaa
     3181 caccgaagcg gccggcatgt cgcccatcga agtcgagcag caggtcaccg cgcgcatcga
     3241 gctcgcgatg ggcgggattc cgggcgtcga ctacatgcgt tcgctgtccc gatacgggct
     3301 ctcgcagctc accatcgtat tccacgacga cgtcgatatc tacttcgcgc gccagctcgt
     3361 gagcgagcgc ctgcgcgagg ccagcgaagg actgcccccc ggaaccgaac agcccgcgat
     3421 ggggcccatc gcgaccgggc tgggcgaaat ctacatgtgg gcggtggaag cggaaccggg
     3481 cgcgctgcga cccgacggcc agcctttcac cgctacggat ttccgcgagg cgcaggactg
     3541 gatcgtcaag ccgcagctgc ggaccgtacc gggcgtcacc gacgtcaact cgatcggcgg
     3601 ttacgtgaag cagtaccacg tgactcccga ccccgcgaag ctggtggcgc acggactcac
     3661 gttccgcgac atcctggacg cgctgtggca gaacaacctc aacgccggcg cgggttacat
     3721 cgagcacgtc ggcgagcaat acctggttcg cattcccggc gtggtgacca ccctcgacga
     3781 cgtcgagaag atcctggtcg gagtggaaga cggcacgccg gttcgcgttc gcgacttggc
     3841 ggcggtcggg ctcgggcagg agttgcgaac cggtgccgcc accgagaacg gcgaagaagt
     3901 cgtgctcggc accgtgttca tgctagtcgg cgccaacagc cgcacggtgt cgaaggcggt
     3961 gggcgcgcgc atggagcaga ttcagggcac gctgccggag ggcctcaagg tgcgcaccgt
     4021 ctacgaccgc acccgtctgg tcgacgcgac cctgcatacc gtacgcgaaa acctcaccta
     4081 cggcgccgtt ctggtcgtcg tcgtcttgtt gctgctgctc ggcaacgtgc gtgcggcgct
     4141 gctcgtggct ctcacgattc cactcgcagg actgttcgcc gtcaccggca tggtacagaa
     4201 ccgcatcagc ggaaacctga tgagtctggg tgcgatcgac ttcggaatca tcgtcgacgg
     4261 cgcggtggtg atggtcgaga acatggtgcg gcgcttcggg aggcgccagc acgagttggg
     4321 ccggctcctg acgcgcgaag agcgcctcgt cgaagccttc cagagcgcgc gcgaagtggc
     4381 caagccgacg ctgttcgggg tcgcgatcat catgatcgtg tacttgccga tcctgacgct
     4441 cggcgggatc gagggcaaga tgttcaagcc gatggcgctc acggtgctcc tcgcgctggc
     4501 cggcgcgctc attctcacct tcacgttcgt tccagcgggt gtcgcgatct tcttgcgcgg
     4561 gcgggtggcc gaacgcgaga gcgcggtcgt gcgcggcacc aagcgcctct acgcgcccgc
     4621 cctgcggcac gcgcttcgat ttcgatggcc tgtgatcgga gcggccgccc tgctggtggc
     4681 cggctgcctg ctcctgggca cgcgcatggg cagcgagttc attccctcgc tcgacgaagg
     4741 cgacatcgcc atgcacgcca tgcgcatccc ctcgaccagt ctcacgcaag cggtcgcgat
     4801 gcagcaccag ctcgaggaga aaatcgaaac ggtacccgag gtggagcgtg tgttcgcgaa
     4861 gatcggcacg gccgagatcg cgaccgaccc catgccaccc tcggtcgcgg acgtgttcat
     4921 catcatgaag ccgcgcagcg ggtggcccga ccctcgtaag tccaaggacc agctcgtccg
     4981 cgagatcgaa gaactcgtga gccacgtccc cggcaacaac tacgagttca cgcagcccat
     5041 cgagatgcgc ttcaacgagc tcatcgccgg cgtgcgcagc gacctggccg tcaagatatt
     5101 cggcgacgac accgacgtca tgctggagga agcgcgcaag atcgcggaca tactcggtga
     5161 tatccccggc gccgccgacg tgaaggccga gcaagtgacc gggctcccgc tcatgacgat
     5221 cgtcgtcgat cgcgacgcga tcgcgcgcta cgggctggac gtggccgacg tccaggacgt
     5281 catcgagatc gcgatcggcg gcaagtcggc gggtcacgtc tgggaaggcg accggcgctt
     5341 cgacctcgtg gtgcgcctgc ccgaagaagc gaggctcgac ctgcacgcgt tggagttcct
     5401 gcccatcccg gtgccggcgg gcacgggccg cgcggggacc tcggccgcgt cgttggacgg
     5461 gattcgccac ctcgaggcgg ggtacctgcc gttgagcgcg gtcgcggaga tccggatcgc
     5521 ggagggtccc aaccagatca gccgcgagaa cggcaagcgg cgcatcgtgg tgcaagccaa
     5581 cgtgcgcggc cgcgacctcg gctcgttcgt cgccgaagcg cagcgacgcg tcgaagccgg
     5641 tgtcgacctc cccgcgggct actggatcga atggggcggg caattcgaga acctgatcgc
     5701 ggcgcgcaaa cggctggcca tcgtggtccc gctctcgctc cttttcatct tcacgcttct
     5761 ctttgcgacc ttcaactcgg tgaagcacgc gctcatcgtg ttctccggcg tccccctcgc
     5821 cttgaccggc ggcattctgg cgttgtggac caggggaatt ccgttctcga tttccgccgg
     5881 cgtggggttc atcgcgctct ccggcatcgc ggtgctcaac ggactcgtca tggtgacgtt
     5941 catcaactca ctgcgccggg atggcgcggc cctggacgac gccatcaccc agggcgccct
     6001 ctcgcgcctg cggccggttc tcatgaccgc cctggtggcg gccctggggt tcgttccgat
     6061 ggccatcgcg accggcagcg gcgccgaggt gcagcgcccg ctcgctaccg tcgtcattgg
     6121 aggaatcgtt tcctcgacgc tcctgacact ggtcgtgctt ccctcgttgt accgggtgtt
     6181 ccatcgaacg cgaccgatac cgccgcgcgc cgtgtcataa agaccaccgc tcccgggttc
     6241 caacttcgga tcgcgccttg ccggtgagcg aagacggcac tactttaggt gcagcctccg
     6301 cagatcgcct acctgcgacg cgacatcacc accccttgag aaggatcgac gggttctatg
     6361 acgcgttgcg cagggatcgc tctgtgctgg attctccttt cgttgacacc cacggggtcg
     6421 ttcgccggcg gcaccgcgac cgccaacgaa gccgcctcgc cgttgatcgg cgatgacggc
     6481 ggtctgggga gcgacgtctt cgacgcggtc ggcagcaccg cgctccacct cggcactgat
     6541 ttttacgagc agctgtcgta tcccttcgtg ctcgcgggga gggatcggtc ccggtttctg
     6601 ctcggttcgg ccgggatcgt cgccctgatc ctcaccgatc gtttcaccta ccagccgatg
     6661 gcgcacccgc cgttcctccc cgacgaaagc ctcgtgggtc cggcacgcac gctctccaac
     6721 ctgggaaaca cgcgcaacgc gattccgctc gtactcggct tcggcgccgc gggtttgatt
     6781 accggctcgc accgcgagaa acagaccagc atcatgctgg ccgaagctct ggtgacgtcg
     6841 ggtgtgtgga cgagcctcct caagtatgcc tcgggacgcg agcgaccgcg cgagatgcag
     6901 gaagccgtcg ccgactggac cgggcccggc ggcgccttcg ccgacgacga cacgcgcggc
     6961 ggacacgcgt cgttcccctc cggccacgcg accggcatct gggccgcggc gacggtgctc
     7021 gcccaccagt acccccaggg ccgcgtcgta cccatcgccg cgtacggcac ggcggccgcc
     7081 atcagctact cgcgcatggt ggtcggagcc cacttcctgt ccgacgtcgt cgtgggcggc
     7141 ctgatcggct acggttgcgc gcgccaggtc atcggggcgc acgagcgctc ggagggcccg
     7201 tcgcgcttcc acctcctgta cgagcccagc ggagacgaac agcgcgtcgg gctggccatg
     7261 gatttctgac cgattcgcgg gcgctcgggc cttttgacaa cgaccgcgaa cacgcattac
     7321 actcggagcg ccgccatcgc cggggcctcg ccctttgcgc gttgtcttcc acagaagggg
     7381 cttcacttgg ccgtcaatca caaacccgac ctcgaccacg ccttccgttc gcagtccgag
     7441 cacttcgtcc attgggagaa gctcaaggac tgcatcgacc agtacatcga catcatgctg
     7501 aactaccgcc agagcggtca cccgggcggg tcgcgctcga aggtgcacgc gatggtgtcg
     7561 ctgctgttga gcggcgccat gcgctgggac atccggcacc ccgaaaagcg attcggcgat
     7621 cgcttcgcgc tggtggcggg ccacaccgtc ccgctcatct acgcgaccct cccggcgctc
     7681 gcggagacgc tgcgggtgaa gcacgagcgg acccagaacc cgcgctactt catccccaaa
     7741 gaacgcatgg tgtggtggga agacttgtcc atgctccgtc gcaacaaggg actccccggc
     7801 cacgccgaga tggaaggcaa gacactcttc gtcaaggcca acacgggtcc gtcggggcac
     7861 ggctcgccgc cggccgcggg acaggcgttc gcgctcaaac gcgcgggcgc cgagggcgtg
     7921 cgtgtgttcg cgttcgaggg cgaaggcggt ctcacccctg gggccgcgca cgagaccatg
     7981 aattcggcct acggcctggg gctcaccaac ttccactacg tcgtcgattg gaacgacttc
     8041 gggatcgacg acgtcgccta cagccacttc gtgtacggga cgccgcgcga ctggttcggc
     8101 gcgcacgggt ggcgcgtgag cgaagccaag gacggcagca gctgggaatc ggtgacgcgc
     8161 gccatgttcg acatggtgat gggcgacaac cccgagcgcc ggcccagcat gatgttcatg
     8221 cgcacgcgca agggccgcga gtatttgaaa tacgacaaca agtcgcacgg cacgccccac
     8281 ccgatgaaca gcgaactatt ctggggcacc aagaagccct tcatggagaa gtacggcgtg
     8341 tcgttcgcgg gtttcggaga gcccgcgccc aaggaggtct ccgaggtcgc cgcacagtgc
     8401 cgggccaacc tcgaggtcgt cgccggcgtg attcgctccg acagcgccct ggtcgattat
     8461 gtggcggaga gattagtgga gatcggcgag tcggtccccg gcgacattgc gggcttcaag
     8521 ctcgacgtct ccaagaaccc cgggcgcgat gccaagatca ccgacttcaa gagctacccg
     8581 gagtcgatgt gggccaagcc gggcgagaag caacccaacc gcgccgcgct cgcgcgctgg
     8641 ggcgcgtggg tgaacgcgta ctgccgctcc cactacgacc gtccgctctt tattgcgatg
     8701 agcgccgacc tcgccgagtc gaccaacatc gcgggcttcg cgaaggagtg ggaggacgtc
     8761 aagggctacg ggtggttcga ccgcgacaag aacctggacg gcatcctgtt gccgcagggc
     8821 atcaccgagt tcaccaactc cggcatcgcg accggcatcg cgagcgtcaa cttctcgaaa
     8881 acaccctacg aagacttcaa cggcttctac gcgacctgct cgacctacgg atcgttcgtg
     8941 tacctcaagt acgggccgat gcgcctgttc agccagctcg cgcaggactc gcagatcaag
     9001 gtcggcaagg tcttgtggat cgcggggcac agcggccccg agaccgcgga ggactcgcgc
     9061 acgcacttcg gcatcttctc ccccggtgtg acgcagctct tccccgacgg ccacgtcatc
     9121 gacgtgcacc cgtgggagtt caacgaagtg ccggtggtca tcgcggcggc cttgaagacg
     9181 agcgcgccca tcatcgctct gcacttgacg cggcccgcgg tcgagattcc cgaccgcgcc
     9241 aagctcggca tggcgtcgca cttcgaagcg gcgcgcggcg cctacgtcat tcgcccgtac
     9301 cgcaaggacc agaagaaaat gggcgtcgtg gtggtacagg ggacgtcgac cacgaacaac
     9361 gtggtgaaga tcttgccgaa gctggacgaa gcaggactga atgtgaagat cgtcgcggcg
     9421 atcagccccc agctgttcgc gcttcaggac tccgcgtatc gggattcggt cttttccgcg
     9481 ctcgatcgtg cggatgcgat ggcgatcacc aaccgcgcgc ggcgtttgat gaacgactgg
     9541 gtcgactacg ggctcaccgg cgagtactgg ctctcgagcg actgggacaa ccgctggcgc
     9601 accggcggga gcgtggacga agtggtcgaa gaggcgcacc tctcgcccag gtggattttg
     9661 gaagggattc gacgcttcgc cgcggaccgc gcgcagcgcc tgcagaagca gcgcgcgcgc
     9721 atcgacgagc ttgcctaggc gaccgcgcgc ggctcctcgc cgatgttgcg cgccgccttc
     9781 gccaccagcg attcgaggaa gcgacggtcg tcgtcgctga aggcatccag ctgatcgctg
     9841 tcgatgtcga gcacgccgac gatgtgaccg cggtccacga tcggcaccac gatttcggac
     9901 ttcacgctcg ggctgcacgc gaggtagcgg tcgtcggcgc acacgtcggg gaccacgatg
     9961 gtttcgcgct cgcgcgcggc cgcgccgcac acgccctcgg agagcgggat gcgacgatgc
    10021 tccggcgagg cgcccacgaa ctcggcgtcg tcgagcgaga gcagttcgcc ctgcagccga
    10081 tagatcccca cccacgagaa gcggtcgaag cgatccttga gctcgtgcac caaggccccg
    10141 gccgcgtcgc gcgacccctt cgctccgcgc acccgacgca gaatcgcatt ctcgacgtcg
    10201 gtggaaagcg cgccttggtc gaacgcgtga ccaaccggcg cgtgcccgcg ttccggcgcg
    10261 ggtgccgcgt cgcgcgcgcg tgcgtcacgc ttcatgagga cgaagcccgc gagcaccagg
    10321 accagcggcc accagcgcag gaagggctgc gcgacgcgtg cactgatggt accggtctcg
    10381 tacagcatac aggccgttcc catcaggatc accatcgagc ccacgaccac gcccgcgctg
    10441 gtcggcggcc ggcgcgcgcc cgccaccacg aacgacacgc cgatgatcat cggaataatg
    10501 ggccatagcg accgcatgcg tccccattcg atcaagccga aggagaacgc cgtgaggaac
    10561 agccccagga gaatcatcca cgcggccgcg aagatgaccg cgaacgtcaa acggctctgc
    10621 agcacgcgca gcatcaccac gccgctcacc accaggatgc acggccatag gtgtttccag
    10681 cccaggagga cgtgattgct cacgagcagg agcaggatgc cccagaccac gagccacgtt
    10741 cccaccagca tgcggttcct cgggttgccc atggggctat catatagaac ggcgcgcaac
    10801 cttgcaacga agcgtgcatc gccctagtat gtcggcgtgc cgcaggaact gggtcgaacg
    10861 ctgatcgtga tcggggccgt cctcttggtg gtcggttgtg ccctcgcgtt cgcggatcgc
    10921 atcccgttcc tgggccgcct cccgggcgac gtggtggtca agaagaggaa cttcaccttg
    10981 tacgtcccga tcgcgaccgg cctgctcctg agtctcatcc tgaccgttct tctcaatttg
    11041 tggtcgcacc gcggggggca gtaaggacgc ccacaacgcc gccgaccgcc gccttgacgc
    11101 ggtctccgcg atcccctaca ttcacgcgaa cggcgggccg acgggccgtc gcggtcattt
    11161 ttccgactcg gaggatccat tcaatgaaac agctcgtggc agcggcggcg ttggccgccc
    11221 tgatcgccgg tgcggccggt gcgcaaacaa ccaagaagga gttacccgtg gtcgttttcg
    11281 agacgacgaa aggcaacatc gagatcgagc tttaccccga caaggcaccc gtgacggtgc
    11341 agaacttctt gtattacgtc gataac
//
LOCUS       DASPGK010000019         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_122606, whole genome shotgun sequence.
ACCESSION   DASPGK010000019 DASPGK010000000
VERSION     DASPGK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122606"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VEC56_00450"
     CDS             complement(<1..691)
                     /locus_tag="VEC56_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12648.1"
                     /translation="MKSMRRLVFFVSLLCIGGWCASASLAAPLAPSADPVTSFITVAD
                     SVARTQGDEGLAAFVADNAVLVGASVAKLVDVAFQLAQGGDAVAAAENVAFAKKIAGA
                     HEAHGGTAVARGIVEAYEKWTPAQRKSRARAMALEEEAAAARKAGDLTKAVALLDQAR
                     AIYEKISDAHSVAVNWGTRGLTNFSTGVWDVVIQDYEKALAARRAVEDRILEGRTLNG
                     LGSAYQQRGDYP"
     gene            complement(925..1839)
                     /locus_tag="VEC56_00455"
     CDS             complement(925..1839)
                     /locus_tag="VEC56_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXV12649.1"
                     /translation="MRYAFWTVDVFTDRSFGGNPLAVIPYANGLSTEQMQSIAREFNL
                     SETAFVLPPENPEHTFRLRIFTPARELPFAGHPTIGTAFVLGLCGRVELKSESTRVVL
                     GENVGPVPVEIRSAGGKPVFTQLTTARLPEWGPSPPPSEIVAAMLSLDPADVLDDGHD
                     EPQAISCGMPFLFVPLASLEAVKRARLNFEWWNQYLSSAWAPQIYAFSFETETSGATI
                     HARAFTHAVGVEEDPATGSAASGLAGYLARRERLRDGTTRWRVEQGIEMGRPSVIDVE
                     AEARDGAVTAVRVGGSSVVVSEGHLLVP"
     gene            complement(1877..3142)
                     /locus_tag="VEC56_00460"
     CDS             complement(1877..3142)
                     /locus_tag="VEC56_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV12650.1"
                     /translation="MTRRVVAVCVLVALIVGSCAREQKPANLVLITVDTLRPDRLGYN
                     GHSRPTSPRIDRLASEGVVFTNATSVSGWTLPSVATIFTGHYPKDHGAVDFHWSLDAS
                     LPTLAGILRARGYDTYGFVSHIMLTPTYGMGEGFSTFDSSVLNVGHPHEVLTSKDLTE
                     IALRGMRNLKEPYFVWVHYFDPHFAYLEHPQYAFGKQDIDRYDAEIAYTDYYIDDLLK
                     VTDRGNTVVVFTSDHGEEFGEHAGQYHYTLHGEVMRVPLVIKAPGLTHRTDDHVVEQI
                     DFLPTILKMLGVEAPAGLPGRDMFAADAAPRNLFLERDRPPQWRQRGVVAGNYKLIVI
                     EEADITQVPESGRAEEIEVTNVHPGMYLYDLSRDPGETTNLFARDDSTSLSLLGALTT
                     HFATASARPQPVQVDQELLEKLRSLGYVR"
     gene            complement(3139..3918)
                     /locus_tag="VEC56_00465"
     CDS             complement(3139..3918)
                     /locus_tag="VEC56_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXV12651.1"
                     /translation="MSRVPDWLVHRPIAHRGLHDVAHAIAENSLPAFWAAARAGYPVE
                     LDVRLLADGEVVVFHDRELDRLTNASGPIAARTSEDLTSLTLLGASIGDDPKREDARI
                     PLLHDVLDLIAGATPLLIEIKNEGEVGALEGATVAALEGYNGPFAIQSFNPGTVQWWR
                     DTAPGVPRGLLAGDFRFEPVDDATRRRLQNLEDLAACEPDFIGYDIRLLPFERVARER
                     KAGRPVIGWTARSLDEATRALEFCDNVIFEGFDATRLRSAT"
     gene            complement(3915..>4247)
                     /locus_tag="VEC56_00470"
     CDS             complement(3915..>4247)
                     /locus_tag="VEC56_00470"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXV12652.1"
                     /translation="DGMEPNRASFGNVVVKPDKSAGMLYVYRFPMAPEDKEYQLWGLR
                     DGLPPVSLGMFSVNPDGTAMLNMKSIPEATHIVGFSVTIEPKGGMPQPTGMMYVKGMD
                     PMEETRDG"
BASE COUNT          714 a         1385 c         1440 g          708 t
ORIGIN      
        1 gcggatagtc gccgcgctgc tgatacgccg atcccagccc gttcagcgtg cgaccctcga
       61 ggatgcgatc ctctaccgcg cgccgcgcgg cgagggcctt ctcgtagtcc tggatgacga
      121 cgtcccagac gcccgtcgag aagttcgtca ggccgcgcgt gccccagttg acggcgaccg
      181 agtgcgcgtc gctgatcttc tcgtagatcg cgcgggcctg atcgagcaac gcgaccgctt
      241 tcgtcaggtc gccggccttg cgggccgccg cggcctcttc ctcgagtgcc atcgcgcgcg
      301 cgcgactctt gcgctgcgcc ggcgtccact tctcgtaggc ctcgacgatg ccgcgcgcga
      361 ccgcggtgcc gccgtgcgct tcgtgcgcgc ccgcgatctt cttcgcgaag gcgacgttct
      421 ccgcggcggc gacggcgtcc ccgccttgcg cgagctggaa cgcgacgtcg acgagcttcg
      481 cgaccgaggc gccgaccaag accgcgttgt cggcgacgaa cgcggcgagt ccttcgtcgc
      541 cttgggtgcg cgcgacggag tcggcgacgg tgatgaaact cgtaacgggg tcagcggagg
      601 gggcgagcgg ggcggccagg gaagcggacg cgcaccaccc ccctatacag aggagagaaa
      661 cgaaaaacac cagccggcgc atggacttca ccgtgcgtac gaatcccttg ccacgctggt
      721 cccacaacga cgtgagacgt caggcggatg cgggtggcgg ttcgggcatc cacctgtact
      781 acctgaatga cacgagatgg ttggcaacgg cacgcgggcg cgcggctgct gatcgcgctc
      841 ggccgtcgac acagtctcgg gatagaagct gcgtgggagg gattatagca gatttgggcg
      901 ttgcggcggg ccgcgttact cgcgctacgg caccaacaaa tgcccttcgg acacgaccac
      961 cgaagacccg ccgacccgca ccgcggtgac ggcgccgtcg cgagcctcgg cctcgacgtc
     1021 gatcacgctg ggacgcccca tctcgattcc ctgctccacc cgccagcgcg tcgtgccgtc
     1081 tctaagtcgt tcccgacggg caagatagcc cgccaaaccg ctggccgcgg agccggtggc
     1141 ggggtcttct tcgaccccga ccgcgtgcgt gaacgcccgg gcgtggatgg tggccccgct
     1201 ggtctcggtc tcgaacgaga aggcgtagat ctgcggcgcc caggccgagg acaggtactg
     1261 gttccaccac tcgaaattga gacgggcgcg tttcaccgcc tcgagcgagg cgaggggaac
     1321 gaacaggaag ggcatgccgc acgagatggc ctgcggctcg tcgtggccgt cgtcgagaac
     1381 gtcggccgga tccagcgaaa gcatggcggc gacgatctcg gagggcggcg gcgacgggcc
     1441 ccactcgggc aagcgcgcgg tcgtgagttg cgtgaacacg ggcttgccgc ccgcggatcg
     1501 aatctcgacc ggcaccgggc ccacgttctc gcccagcacg acgcgcgtcg actcgctctt
     1561 cagctcgacg cggccgcaca agcccagcac gaacgcggtg ccgatggtcg ggtggccggc
     1621 gaagggaagc tcgcgcgcgg gcgtgaagat gcgcagccgg aaggtgtgct ccggattctc
     1681 gggcggaagc acgaaggccg tctcggagag gttgaactcg cgcgcgatgc tctgcatctg
     1741 ctcggtcgag agtccgttgg cataggggat gacggcgagc ggattaccgc cgaaggagcg
     1801 gtcggtaaag acgtcgacgg tccagaacgc gtagcgcatg ggggcctcct ccaaggcctc
     1861 gatgatagca cgtttgctac cgcacgtagc ccaggctgcg cagcttctcg agcagctcct
     1921 ggtcgacttg caccggttgc gggcgcgcgc tggcggtggc gaagtgggtc gtcaacgcgc
     1981 cgagcagcga tagcgacgtc gagtcgtcgc gcgcgaagag gttcgtcgtc tcaccggggt
     2041 cgcgagagag gtcgtagagg tacatgcccg ggtgcacgtt ggtgacttcg atctcctcgg
     2101 cgcgacccga ttccggcacc tgcgtgatgt cggcttcctc gatcacgatc agtttgtagt
     2161 tccccgccac gacgccgcgc tggcgccatt gcggcgggcg gtcgcgctcc aggaacaggt
     2221 tgcgcggcgc tgcgtcggcc gcgaacatgt cgcggcccgg gagtccagcc ggggcttcga
     2281 cgccgagcat cttcagaatg gtcggaagaa aatcgatctg ctcgaccacg tggtcgtcgg
     2341 tgcggtgcgt gagcccgggc gccttgatga ccaacggcac gcgcatcact tcgccgtgga
     2401 gcgtgtagtg gtactgaccc gcgtgctcgc cgaattcctc gccgtggtcc gaggtgaaca
     2461 cgaccacggt gttgccgcgg tcggtgacct tcagcaggtc gtcaatataa tagtccgtgt
     2521 acgcgatctc cgcgtcgtag cgatcgatgt cttgcttgcc gaacgcatac tgcgggtgct
     2581 ccaagtaggc aaagtgcggg tcgaagtagt gcacccacac gaagtacggc tccttcagat
     2641 tgcgcattcc gcgcagcgcg atctccgtga ggtccttcga ggtcagcacc tcgtgcggat
     2701 ggccgacgtt gagcaccgag gaatcgaagg tcgagaatcc ttcccccatg ccgtaggtcg
     2761 gcgtgagcat gatgtggctc acgaagccgt aggtgtcgta gccgcgcgcg cgcagaatcc
     2821 cggccagggt gggaagcgag gcgtccagcg accaatgaaa atcgaccgcg ccgtggtcct
     2881 tggggtagtg ccccgtgaag atggtggcca ccgagggcag cgtccagccg ctgacgctgg
     2941 tggcgttggt gaacacgacg ccttcgctcg cgagccggtc gatgcgcggg ctggtcgggc
     3001 gggaatgacc gttgtagccc agacgatcgg ggcgtagcgt gtcgaccgtg atcaatacga
     3061 gatttgccgg cttctgctcg cgcgcgcacg agcccacgat aagcgcgacc agaacacaaa
     3121 cagcgacaac gcgacgcgtc acgtcgcgct ccggagtcgg gtggcgtcaa agccctcgaa
     3181 aatgacgttg tcacagaatt cgagcgctcg cgtcgcttcg tcgagcgagc gcgccgtcca
     3241 accgatgacg ggtcttcccg cctttcgctc gcgcgccacg cgctcgaacg ggagcaggcg
     3301 aatgtcgtag ccgatgaaat ccggctcgca cgcggccagg tcttcgaggt tctgcaggcg
     3361 ccgccgcgtc gcgtcatcga cgggctcgaa acgaaaatcg cccgcgagta gcccgcgcgg
     3421 tacgccgggc gcggtgtcgc gccaccactg cacggttccc gggttgaacg actggatcgc
     3481 gaagggaccg ttgtagcctt ccagcgcggc gacggtcgcc ccctccagcg cgcccacctc
     3541 gccttcgttc ttgatctcga tcaagagcgg tgtcgcaccc gcgattagat ccagtacatc
     3601 atggagcagt ggaatgcgcg cgtcctcgcg cttgggatcg tcgccgatgg aagcgccgag
     3661 cagcgtcagc gacgtcagat cctcggaggt gcgcgcggcg atggggccgg acgcattcgt
     3721 cagtcgatcg agctcgcggt cgtggaacac caccacttcg ccgtcggcca gcaatcgcac
     3781 gtccagttca accgggtagc ccgcgcgcgc ggccgcccag aacgccggca gcgaattctc
     3841 cgcgatcgcg tgcgccacgt cgtgcagacc gcggtgcgcg atgggacggt gaaccagcca
     3901 atcgggaact cgactcatcc gtccctcgtt tcttccatcg gatccatccc cttcacatac
     3961 atcatgccgg tcggctgcgg catgccgcct ttgggttcga tcgtcaccga gaagcccacg
     4021 atgtgcgtcg cttccggaat ggatttcatg ttgagcatcg cggtcccgtc ggggttgacc
     4081 gagaacatgc cgaggctcac gggcggcaac ccgtcgcgca aaccccacaa ttggtattcc
     4141 ttgtcctcgg gcgccatcgg gaagcgatac acgtacagca tgcccgcact cttgtcgggc
     4201 ttgacgacca cgttgccgaa cgaggctcga ttgggctcca tgccgtc
//
LOCUS       DASPGK010000020         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_6034, whole genome shotgun sequence.
ACCESSION   DASPGK010000020 DASPGK010000000
VERSION     DASPGK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6034"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VEC56_00475"
     CDS             complement(<1..1283)
                     /locus_tag="VEC56_00475"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXV12653.1"
                     /translation="MTAAVFWPVAAHSAKVECEIVGVSDPEVVENIEATLSIVRDQDR
                     DDLTAERISRLHERAPTEIQKAVEPFGFYRAVVEGSLTPGKKDRFRARYVVTLGEPVR
                     VQEVSVSVTGDGKDAPPFPALVARFPLGPGDVLDQRLYSRAKTTFASTAADSGYLDAT
                     FTASTIRIHREENVADVEIAFDTGPQYRFGAVVFDSTAIDDRVLRAYVTFKRGDPFRY
                     SKLLRFQSALGGAPYFSRVEAVARRDIAVNHEVPIQVKLALRRPRRYEVGVGYGTDTG
                     PRILFNAEFRRLNRVGHHYRGKLNVSEVELSLYADYVMPSLYPKTHTYTIGALAARID
                     PDAYTTDRFAIGPTRSQPRLGWLESITLSYEWEDYTVGSDEGTTDLVIAGLSYRYKRA
                     DDDIAPTHGHRLDLGLRGAGQDFLSTQSFLSLTGSV"
     gene            complement(1412..2191)
                     /locus_tag="VEC56_00480"
     CDS             complement(1412..2191)
                     /locus_tag="VEC56_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12654.1"
                     /translation="MTPEIPAYITTLVIAAAFAIAIAVTMALSRAAGRSGLPHASQRR
                     VRLGSMVFFGVWLAAVMLLAPTSSPPASVDSFAIAPLIPLAAVGSLAVALVAVARSAS
                     LRRVLLSVPLATLNALQVWRVVGILFVILLAQGVLPAHFALPAGWGDVAVGVTAPWVA
                     LALSRGVRGSAAIAIGWNVFGLLDLVVAVGMGTGLLAPVLVPELGPRVPPAAAMGMLP
                     MILVPTFAVPASVMVHVLALHRLRRDAQSAPAARGALIARS"
     gene            complement(2220..>3533)
                     /locus_tag="VEC56_00485"
     CDS             complement(2220..>3533)
                     /locus_tag="VEC56_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV12655.1"
                     /translation="ENTFQLMYPTGGFGGMVTKPWSERSKSTEQLQMESSVGLSQIAG
                     VRVIPLIPPALPGGGDFPVDFVIASTAEPEQLIEFANQLVMKAFASGMFMFADADLKF
                     DQPQTEVVFDRDKVRSIGVDMSRAGRDLSTLLSGDYVNRFSIQGRSYKVIPQIKRSER
                     LTPAQLEEIHVTGPEGRLVPLSTFASLRTTTEPRELKRFQQLNAVRIQGVIPPPVSLD
                     AALRFLETEAAAILPPGYAIDYAGESRQLRTEGGKFLATFLLSAILIYLVLAAQFESF
                     RDPFVILVGSVPLAISGALLFSFLGLTTLNIYSQVGLITLVGLVAKNGILIVEFANHL
                     QETGVSKLHAVIEAAGTRLRPILMTSAATVVGHMPLVFASGPGAGARNSIGIMLVTGM
                     VIGTVFTLFVVPSVYMLVAKTRKATAAAPQTSEPDGGSEVAVAAA"
BASE COUNT          643 a         1159 c         1228 g          503 t
ORIGIN      
        1 accgacccgg tgagcgagag gaagctctgc gtcgacagga aatcttgccc cgcgccgcgc
       61 agtccgaggt ccaagcggtg gccgtgcgtg ggcgcgatat cgtcgtccgc gcgcttgtac
      121 cggtacgaca gccccgcgat gacgagatcc gtggtgcctt cgtcggatcc caccgtgtag
      181 tcctcccact cgtaactcaa cgtgatcgac tcgagccagc cgaggcgcgg ctgcgagcgc
      241 gtgggcccga tcgcgaagcg gtcggtcgtg taggcatcgg ggtcgatgcg cgccgccagc
      301 gcgccgatgg tgtacgtgtg cgtcttgggg tagagcgacg gcattacgta gtccgcatag
      361 agcgagagct ccacctccga cacgttgagc ttcccgcggt agtggtgccc cacgcgattg
      421 aggcggcgga actcggcgtt gaacaggatg cgcgggccgg tgtcggtgcc gtagcccacg
      481 cccacttcgt agcggcgcgg gcgccgcagc gcgagcttca cctggatggg aacctcatgg
      541 ttcaccgcga tgtcgcggcg cgcgaccgct tccacgcggc tgaaatacgg ggcgcccccc
      601 agcgccgatt ggaacctgag cagcttgctg taacgaaacg gatcgccgcg cttgaacgtg
      661 acgtaggcgc gcagaacgcg gtcgtcgatc gcggtggagt cgaacaccac cgcgccgaag
      721 cggtactgcg gcccggtgtc gaacgcgatc tcgacgtcgg cgacgttctc ctcgcggtgg
      781 atgcggatgg tgctggcggt gaaggtcgcg tcgaggtagc cgctgtcggc cgcggtgctg
      841 gcaaaagtgg tcttcgcgcg cgagtacaaa cgctggtcga ggacgtcgcc gggcccgagc
      901 ggaaaccgcg ccaccaatgc gggaaacggc ggcgcgtcct tcccgtcacc ggtgacggag
      961 acggagacct cttgcacgcg caccggctcg cccaatgtaa cgacataacg cgcgcgaaat
     1021 cggtccttct tgccgggagt gagcgagcct tcgacgaccg cgcggtagaa gccgaacggc
     1081 tccaccgcct tctggatctc ggtcggcgcg cgctcgtgca ggcgcgagat tcgttccgcg
     1141 gtgagatcgt cgcggtcctg gtcgcggacg atcgaaagcg tggcctcgat gttctcgacg
     1201 acctcggggt cggagacgcc gacgatctcg cactcgacct tggccgaatg cgcggccacg
     1261 ggccagaaga ccgcggcggt caacgcccat gccgcgacga tcgcgcggcg acggagacgg
     1321 ttgcagagaa cggggtgcat agcctgaacc ccccagcgta accgacgcgt gcacgcggtg
     1381 tccacgcgcc gaggccggag gtccaggccg gttacgaccg cgcgatgagc gcacctcgtg
     1441 cggcgggggc ggactgtgcg tcgcggcgca ggcgatggag agccagcacg tggaccatga
     1501 ccgaggccgg cactgcgaac gtgggcacga ggatcatggg gagcattccc atcgcagccg
     1561 cgggcggaac gcggggtccg agttcgggga caagcaccgg cgcgaggaga ccggtcccca
     1621 ttccgaccgc gacgaccaga tcgagcaaac cgaagacgtt ccacccaatc gcaatcgccg
     1681 cgctgccgcg cacgccgcgg gagagcgcga gcgcaaccca tggggccgtg acgccgaccg
     1741 cgacgtcgcc ccaaccggcc ggcaacgcga agtgggccgg cagcacgcct tgcgcgagga
     1801 ggatcacgaa caggatcccg accacgcgcc aaacctgcag cgcattgagt gtcgcgagcg
     1861 gaaccgacaa gagcacgcgc cgcagggatg ccgagcgcgc gacggcgacg agcgccaccg
     1921 cgagcgatcc gactgccgcg agcggaatca acggcgctat ggcgaaggag tcgacggagg
     1981 ccggcggcga tgaagtgggt gcgaggagca tcaccgccgc cagccacacg ccgaaaaata
     2041 ccatcgagcc gagacgcacc cggcgctgcg aggcgtgcgg caacccactg cgacccgccg
     2101 cgcgcgacag cgccatggtg accgcgatcg caatggcgaa agcggccgcg atcacgaggg
     2161 tcgtgatgta agcgggtatt tcgggagtca tgactaagct cctttcctct cgccctgcgc
     2221 tatgcggcgg ccaccgcgac ctcggagccc ccatcaggct ccgaggtctg gggcgcggcg
     2281 gccgtggcct tgcgcgtctt cgccaccagc atgtagaccg acggcaccac gaacagcgtg
     2341 aacacggtgc cgatcaccat cccggtcacg agcatgatcc cgatcgagtt gcgcgcaccc
     2401 gcaccgggac cgctcgcgaa cacgagcggc atgtgaccga ccacggtggc ggcactcgtc
     2461 atgagaatgg gacgcagccg ggtgcccgcg gcttcgatga ccgcgtgcag cttggacacc
     2521 ccggtctcct gcaaatgatt cgcgaactcg acgatcagga tcccgttctt cgccaccagc
     2581 cccaccagcg tgatcagtcc cacctggctg tagatattga gcgtggtgag cccgaggaac
     2641 gagaacaaca aggcacccga gattgcgagc ggcaccgagc ccaccaggat cacgaaggga
     2701 tcgcggaagc tctcgaactg agcggccagc accagataga tgagaatggc cgagagcagg
     2761 aaggtcgcga ggaacttgcc gccctcggtg cgcagctggc gcgactcgcc cgcgtagtcg
     2821 atcgcgtagc ccggcggcag gatggccgcc gcttccgtct cgaggaagcg cagggccgcg
     2881 tcgagcgaca ccggcggggg aatgacgccc tggatgcgga ccgcgttgag ctgctggaag
     2941 cgcttgagct cgcgcggctc ggtcgtcgtg cgcagcgacg cgaacgtcga cagcggcacc
     3001 aagcgtcctt ccgggccggt gacgtggatc tcctcgagct gcgccggtgt gagccgctcg
     3061 ctgcgcttga tctgcggaat aaccttgtag ctccggccct ggatgctgaa gcgattgacg
     3121 taatcgccgg agaggagcgt ggagaggtcg cgcccggccc ggctcatgtc gacgccgatc
     3181 gaacgcacct tgtcgcggtc gaagaccact tcggtctgcg gctggtcgaa cttcaagtcg
     3241 gcatcggcga acatgaacat cccgctcgcg aacgccttca tgaccaattg attcgcgaac
     3301 tcgatcagct gctcgggctc cgcggtggac gcgatcacga agtcaaccgg gaagtcgccg
     3361 ccgccgggga gcgcgggcgg gatgagcggg atcacgcgca cgcccgcgat ttgagagagg
     3421 cccacggacg actccatctg gagctgctcg gtgctcttgc tgcgctcgct ccatggcttg
     3481 gtgaccatgc cgccgaagcc gcccgtggga tacatcaact ggaacgtgtt ctc
//
LOCUS       DASPGK010000021         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_942602, whole genome shotgun sequence.
ACCESSION   DASPGK010000021 DASPGK010000000
VERSION     DASPGK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942602"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..2181
                     /locus_tag="VEC56_00490"
     CDS             145..2181
                     /locus_tag="VEC56_00490"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXV12656.1"
                     /translation="MAEFGSFAVVLALTFCIYAIFALFYGAKSGRRDLVKSGEHAVFA
                     VFVFVTCAVIALEALLLKSDFSIEYVAGYSNRDLPVFYKLAALWGGQKGSLLFWSWIL
                     TMYGALVALRNRRYAGKLIPYTLGILCVTSAFFLIMNTFAANPFERLAMDHGDGVKHA
                     FAPPDGKGLNPLLQHPAMVIHPPMLYLGYIGFVVPFAFCLGALLTRELGAGWIRMARR
                     WTLTAWLFLGTGILLGGRWAYVELGWGGYWAWDPVENASLMPWLTGTAFLHSVIVQER
                     KNMLKVWNVVLILATYLLAIFGTFLTRSGIVSSVHAFAQSSIGTYFVVFLILMGLFSI
                     YLVLTRLEFLKSDNQLDSYASREASFLFNNLILLAACFAVLWGTIFPVISELIQGEQV
                     TVGAPFFNRINVPIALFLLFLTGVGPLLAWRKTSFESMRRIFMWPTIVGLVTAGLSVA
                     LGTRSFYAMISFGLAGFVTTTLVSEFSRAARARRRNTGENMLAALIGITATNKRRYGG
                     YIVHFGFVILMIGFTGQAFTTEGWGEVNEGEKFTIGHYEFECLAIEDIEDPNFVGMSA
                     TVAVFKDGKKLATLAPEKRHYAASDQTTSEVRIHHTLREDVYVVFANVNEETGKAVMQ
                     VWINPLVQWVWLGGFVIVLGTMVTLLPNRRERAITRQKEMVERLLAESERKVAS"
BASE COUNT          386 a          765 c          764 g          491 t
ORIGIN      
        1 cgcgcggacg gcacgttcgc ggccgatcac attcaagcca agtgcgcgtc gaagtacgaa
       61 gccaagtacg gcgcgagcgc gcagcacccg agtcaggttc cgatgagcgg ggcggccgac
      121 tcgacgtcgg gcggttcata ctaaatggcg gagttcggta gttttgcggt cgttctcgcg
      181 ctgacgttct gcatctacgc gatttttgcg ctcttttacg gcgcgaagtc ggggcgtcgc
      241 gacctagtca agagcggcga gcacgcggtg ttcgcggtgt tcgtgttcgt gacctgcgcg
      301 gtcatcgccc tcgaagcact ccttctcaag agcgatttct cgatcgagta cgtcgcgggg
      361 tattccaacc gcgatctccc cgtcttctac aagctcgccg ccttgtgggg cggccagaag
      421 ggctcgctgc tcttttggtc gtggatcctc accatgtacg gcgcgttggt ggcgcttcgc
      481 aaccgccgct acgccggcaa gctgattccg tacacactcg ggatcctgtg cgtgacgagc
      541 gcgttcttcc tcatcatgaa tacgttcgcc gccaatccgt tcgagcggct ggcgatggat
      601 cacggcgacg gcgtcaagca cgccttcgcc ccgcccgacg gcaagggact caacccgctg
      661 ctccagcacc ccgcgatggt gatccacccg cccatgctgt acttgggcta catcggcttc
      721 gtggtgccgt tcgcgttctg cctgggcgcg ctgctcacgc gcgagttggg cgcgggctgg
      781 atccgcatgg cgcggcgctg gacgttgacc gcgtggctct tcctcggcac cggtatcctg
      841 ttgggcggcc gctgggccta cgtcgaattg ggctggggcg ggtactgggc ctgggatccg
      901 gtcgagaacg cttcgctcat gccctggctc acgggcaccg ccttcctgca ctcggtgatc
      961 gtgcaggagc gcaaaaacat gctcaaagtc tggaacgtgg tgctcatcct cgcgacctat
     1021 ctgctcgcga tcttcggcac cttcctgacg cgcagcggca tcgtgtcgag cgtgcacgcc
     1081 ttcgcgcagt cgtcgatcgg aacctacttc gtcgtgttcc tcatcttgat gggactgttc
     1141 tcgatctacc tggtgctgac gcgcctcgag ttcttgaaga gcgacaacca actcgattcg
     1201 tacgcctcgc gcgaagcgag cttcctcttc aataatctga tcttgctggc cgcgtgcttc
     1261 gcggtcttgt gggggaccat cttccccgtc atctcggagt tgatccaggg cgaacaggtg
     1321 acggtgggcg cgcccttctt caatcggatc aacgtgccga tcgcgctctt cctcttgttc
     1381 ctcaccggtg tgggtccgtt gctcgcgtgg cgcaagactt cgttcgagag catgcggcgt
     1441 atcttcatgt ggccgacgat cgtcgggctg gtgaccgccg gcttgagcgt cgccctgggc
     1501 acgcgctctt tttacgcgat gatctccttc ggcctcgcgg gcttcgtaac caccacgctc
     1561 gtgagcgagt tttcgcgcgc ggcgcgcgcg cggcgtcgca acacgggcga gaacatgctg
     1621 gccgcgctga tcggtatcac cgccaccaac aagcggcgct acggcggcta catcgtccac
     1681 ttcgggttcg tcatcctgat gatcggattc acggggcagg ctttcacgac cgaaggctgg
     1741 ggcgaggtga acgagggcga gaagttcacg atcggtcact acgagttcga gtgcctcgcg
     1801 atcgaggaca tcgaggatcc caacttcgtc ggcatgagcg ccaccgtcgc ggtcttcaag
     1861 gacggcaaga agctcgcaac cctggcgccg gaaaagcgcc actacgcggc cagcgaccag
     1921 accacctccg aggtgcgcat ccatcacacg ctgcgcgaag acgtctacgt ggtgttcgcg
     1981 aacgtgaacg aggagacggg caaggcggtc atgcaggtgt ggatcaaccc gctggtgcag
     2041 tgggtgtggc tgggcggctt cgtgatcgtg ctgggcacga tggtgacgct actcccgaat
     2101 cgccgcgaac gcgcgatcac gcgccagaag gaaatggtcg aacggctgct cgcggagtcg
     2161 gagcgaaagg tggcgtcgtg acagcatcgt cgggcgcgta tcatccctcc tccgctcaag
     2221 gtcctcgcgc gagacatctg gtatcgcgct gccgaaatcg cgtcggaggg atgatacgcg
     2281 ccctcctatt gctggtcacg atcgccggcg cgccgttcac cgtcgccttc gcccagtcgg
     2341 ccaagaccca cctcgccgat ccggtgcagg cgaagcggtt caacgagatc tcggaccggc
     2401 tcatct
//
LOCUS       DASPGK010000022        12782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_6444, whole genome shotgun sequence.
ACCESSION   DASPGK010000022 DASPGK010000000
VERSION     DASPGK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6444"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VEC56_00495"
     CDS             complement(<1..151)
                     /locus_tag="VEC56_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12657.1"
                     /translation="MSARTKKVLLVILDGWGWREETEANAVRLANTPTVDRLWERYPH
                     TLIQAS"
     gene            complement(148..2409)
                     /locus_tag="VEC56_00500"
     CDS             complement(148..2409)
                     /locus_tag="VEC56_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV12658.1"
                     /translation="MKRIRLVVIAIAALLLLGAAAGLKRVPADREAVRVGRDGGVAVL
                     ASGWHWVGPGTTLIRYPSGEHSFRVPESDSFHVIFQMGDSLAVAYEFDLAFPPGSSEV
                     LYRQFSKDFEDAFSNLVVSVAEIEAASRTTAAEASDLDAAVVDQVRDEMQALGVEVRR
                     GARARADASMRVESKAPRRLVIVGVDGGDWQNLKPLIDAGRLPNFARLVREGATGPLR
                     SEEPMLSPLLWTTMATGRYPEDHGVLNFTVVDPKTGARVPISRLYRKVDAFWNMLSRH
                     DRRVVVVGWLATDPAEAIDGVMVTDKFGYVAYAPTASRGDSARASAPSSVHPRARAGE
                     LAGMVVHADAITDDQVGRFVQVPHAELSKHRGSFDPKDPVNNLIHLYASTLTFRNIAT
                     TLWQNDQPDVLAVYFEWVDAMSHLFMLHASPRMPDVPEKEYARYSRAIDETYVAQDEI
                     LGEIMSKIDERTVLMVVSDHGFKSGESRLKNRPEIWAGNAALWHRIDGIVAFFGAGVK
                     RGATIDGASILDVAPTILALQGLPRAGDMPGKPLASAFDDAVVATFSSETVATLDGPR
                     GEAHSDAAASSADAETMKKLEALGYLAPDNADAHNNLGQRYQERGEYEKAIAAYKEAI
                     AMRPNFHGAYNNLAVCYGKLKRYDEAEAALRKCIALKPDDFYAMNNLAVMLIETQRID
                     EGLRFAEQAVKTEPAYANGHVTLGSAYAMTRRFDEAEREFREALRLDPANNAAATNLD
                     RLAQARAGAGGNR"
     gene            2460..3320
                     /locus_tag="VEC56_00505"
     CDS             2460..3320
                     /locus_tag="VEC56_00505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV12659.1"
                     /translation="MRVPVPPSVVVVVLTWNGRALTMDCLRSLEAVATPNVRVMVVDN
                     ASTDGTAAAIRERYGERVEVVENASNLGYAGGNNVGIRRALDQGARFVLLLNNDTTVA
                     PDFVDQLLGATLDPAIGIAAPKIYFAEPPDRIWYAGGEISLWRGAARHAGIREHDRGQ
                     HDTARDVDYASGCALLARREVFERVGLLDESYRAYFEDADLCLRAARAGFRVRYVPTA
                     QVWHRISASTGGQVSRAKVRRKLESAWRFFRAHARPYHWITIPLFFALDVVRITGLVL
                     VGRIRDADPL"
     gene            3360..4238
                     /gene="rfbD"
                     /locus_tag="VEC56_00510"
     CDS             3360..4238
                     /gene="rfbD"
                     /locus_tag="VEC56_00510"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXV12660.1"
                     /translation="MKVAIFGVGGQLGRAVAATLATTHDVVSIDHDRADVRHAVAVAD
                     VVRAAQPEWVLNCAAMTHVDRCENDPLAAFEVNALGARNVARAAAAAGARLLHVSTDY
                     VFDGEKPSPYGEDDAVRPINAYGVSKLSGEWFVRADCPAHAIVRTSGLYGWYVCRGKG
                     ANFAETILARARQGQPLRVVSDERLTPTFTEDLAAQLRAIIEGNVPSGVYHATNAGAC
                     SWFDFASELVRLAGVPASIQPISAREWKSPTRRPANSVLENRALAKLGLDRMPDWRDA
                     LARYLAARDDKAGPAA"
     gene            complement(4243..4491)
                     /locus_tag="VEC56_00515"
     CDS             complement(4243..4491)
                     /locus_tag="VEC56_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12661.1"
                     /translation="MIEWFIVIGLVSLVGLFVGAVMLHILVGLVLLPFKLAFAVFKGL
                     VLGLFAIPLAALGLSVAIAFVAIVLALGAAALACAVFF"
     gene            4475..7234
                     /locus_tag="VEC56_00520"
     CDS             4475..7234
                     /locus_tag="VEC56_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV12662.1"
                     /translation="MNHSITRHLPTGPVAHTRGFVPGYPGRGRSFENGAKPRRRWEPF
                     AAIAALVLVASAANAADPPRVMTPDPLPYVSPSRAYDTEHTSLDLDIDLQARRVAGHV
                     THTVRALRSDLSEVRMNCVELMIDSVTVDGRRARFEYPVSAGQSTSWLAGASAGTADD
                     QLAVICDPPLARGAQARVSVYYSGTPEIGLYWIRPEKGIPDKRYEVWSQGEGEDNRYW
                     IPCNDYPNDKATFEGRFRVAKGYTAISNGTLMGTREVGGKTEFHWKLEEPQVSYLIML
                     AVAEYKVLEQKAGTIPLPYVVPPGTDDATILRGYGLTPDMMDYFEKEIGIPYPFSKYA
                     QVVVQDYIYGGMENTTATVMNMRTLYDERAELTRTEQNLVAHELAHQWWGDMVTCREW
                     SHMWLNEGFATYYAYLYKEHRDGDDAFRYQMRQAHNDVIKADDAEPRPMVVDFFNRTD
                     ARNNALVYVKGASLLHMLRAYLGDDLYRETIRHYGEENKHDVVETQDLMRAVREVTGE
                     NLDWFFEQWAFLAGHPKFKVTKSWDRDTRVLTLKVEQTQKTVGVVPVFRVPVDVEIGW
                     SGGSALHRVMIEEASASYYFPVPEEPRSVVFDKGDWILKTVDFPKLAGELVHELENGD
                     FMTRVVAAEALGAKGSDARVVPALRAVVLGDAFWGLRRESALSLGKIGTDAALSALVE
                     GTAVEDARVRLACAEALGGYYRNAKAAAQLEKLVGNDRAYGVRAAALTALVKVRGERA
                     ARVCADALKQTSDQDIVRNAALNGLADLNDTAALDRIRPYCASGNPRWYRHTAIAAYG
                     RLAKQLDKESDRRRAAESLYPALDDWHLRTREAAIDALAAIGEPSSVEPLRRVAANDR
                     LAAVRERAAKAADRIGAESAKTTARNNAADIEELKRRLESVERELEAAKKKLAAPASE
                     AAGSD"
     gene            7412..7672
                     /locus_tag="VEC56_00525"
     CDS             7412..7672
                     /locus_tag="VEC56_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXV12663.1"
                     /translation="MKSDKDRYSWARQAGMLSTIPFLMVVPPIAGLLIGRFIDRKLDT
                     QPAFTIVFLILGFAAGVREVAIVLKRANARDDDGDKRDRSAP"
     gene            7653..8099
                     /locus_tag="VEC56_00530"
     CDS             7653..8099
                     /locus_tag="VEC56_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12664.1"
                     /translation="MIEVRPSGAVWSAIDGDFLRRVRTSSIAFGCVLAIPLATKFGWL
                     AAGAWIAGGAWSIVNLAAIASVMRKVLTLEPRDRGAIVKALAIKFPVLYAIGFGLLAA
                     GLPVMWLLAGFAWPLLVAVLKAAGRSYLRLDETADAPRQGNTRVLS"
     gene            8086..8991
                     /gene="atpB"
                     /locus_tag="VEC56_00535"
     CDS             8086..8991
                     /gene="atpB"
                     /locus_tag="VEC56_00535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXV12665.1"
                     /translation="MFSPSLTRMFDGLAAMRLAAEGHADSAHAVVSHDASHAAAAHAE
                     EHAVHELPNWITLITKFMQDSPAKEFLHQWEGMIWALAIATLIIVIARLAARNPQMVP
                     GRLQNIVEMVVEGLSNFIVGVLGPEGRRYVPFLGTLFIYILSMNLAGVFPLGKSPTAS
                     PNTTVALALCVFVYVHFIGLTRNGPVGYMKHLAGNPRSPVEWAFVPLMLPIHVIGEIA
                     RPVSLSLRLFGNVTGEDVLLFAFCSLGVTTLAFVDSPVGIPFQVPFILLAFMTSFIQA
                     LVFTLLSTIYILLWLPHDEHAEEHH"
     gene            9038..9271
                     /gene="atpE"
                     /locus_tag="VEC56_00540"
     CDS             9038..9271
                     /gene="atpE"
                     /locus_tag="VEC56_00540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HXV12666.1"
                     /translation="MDTSSALSLALPIGLGLAAIGSGIGFQGAIGKAMEAIGRQPEAS
                     GKIQTAMIIGAGLIEALTIYAFVTVFLLMGKIQ"
     gene            9402..9896
                     /gene="atpF"
                     /locus_tag="VEC56_00545"
     CDS             9402..9896
                     /gene="atpF"
                     /locus_tag="VEC56_00545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXV12667.1"
                     /translation="MELQWKALLTQVVGFLVVLWLLRKYAWGKLLAFTEKRRETIANE
                     FANIEKAKVDADALRRRFEEELAGIENTRRTRIQDAAHEANEIASQIREEARRETVDL
                     RVKANQDIAMEMDKANAAFRDQMVNAVIAATDKLIRLRLDDAQHRRLINEFLDEVTTR
                     PASR"
     gene            9904..10467
                     /gene="atpH"
                     /locus_tag="VEC56_00550"
     CDS             9904..10467
                     /gene="atpH"
                     /locus_tag="VEC56_00550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HXV12668.1"
                     /translation="MATAGVGKRYAIALFNAAQAEDVLDQVHGDVTSFGKLLASEEGF
                     RRFLLSGRVTGDEKQGLIVKALGERASGLFVKFVLLLIDKKRTAAYDDIARAFVSLYE
                     EYKGVVEVRVVTAIPLDTELERKAKDVVERRTGKKVKLEKRIEPGIVGGMIMFIENQV
                     FDGSIRNRLGDLGTALLATRVHVVDQS"
     gene            10483..12012
                     /gene="atpA"
                     /locus_tag="VEC56_00555"
     CDS             10483..12012
                     /gene="atpA"
                     /locus_tag="VEC56_00555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXV12669.1"
                     /translation="MAFRPEEVSSIIQAQLQKTSLELEMESVGTILQVGDGIARIWGL
                     EDAMSGELLEFPNGGVGMVMNLEADNVGAVLFGSDTGIKEGDAVKRTGRVAQVPVGEA
                     LLGRVVNSLGQPIDGKGPIVTDTYNPMEPLVPNVVQRQPVKEPLQTGLKAVDSMIPIG
                     RGQRELIIGDRQTGKTAICIDTIINQKGTGVKCIYVAVGQKESTVAQIVSKLEQHGAM
                     EYTTIVSATASESAALQMLAPYAGCAMGEFFRDRGGHAVCFYDDLSKHAQAYRQMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDNLGGGSLTALPIIETQLGDVTAYIPTNV
                     ISITDGQIFLEGDLFYSGVRPAINVGISVSRVGSNAQTKMMKQVAGRLRLALAQYREL
                     AAFARFGSDLDKSTLAQLTRGEKMVEILKQDENVPLATEKQVLLIFAANEGLLDDLPT
                     ESLKRFEPEFFAFMDSKYPDVVRQLREKKAFDKQVEDEARKAAGEFKAAFVKSLPAKK
                     A"
     gene            12025..>12782
                     /locus_tag="VEC56_00560"
     CDS             12025..>12782
                     /locus_tag="VEC56_00560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HXV12670.1"
                     /translation="MATIRDLKNRIRAVENIRKITRAMQLVAAAKLNRAQQRAHAVRP
                     FADELDRVLGGLAGGGIEESADVAIEFAYADGVAPVKTTLGRFFSTHEVKKPGLVLFT
                     ADRGLSGAFNTNLIRAAQTFLREHAHQDVKLITIGRKGYGFFRRRSTPILYTRTGLSD
                     RLLLPEIKEVTGKLLELYVNDEVDALYFIYAKSLRASLYRVTTQQFLAIPAVAGATSD
                     DNYLIEPGRDELFADLIPLYATSRVFSVLADSFAS"
BASE COUNT         2246 a         4263 c         4191 g         2082 t
ORIGIN      
        1 ccgacgcctg gatcagcgtg tgcggataac gctcccacaa gcgatcgacg gtgggcgtgt
       61 tcgccagacg aaccgcgttg gcctccgtct cctcgcgcca gccccagccg tccaggatga
      121 cgaggagcac tttcttcgtg cgcgcgctca tcggttgccc cccgcgcccg cgcgcgcctg
      181 cgcgaggcga tcgaggttcg tggcggcggc gttgttggcc gggtccagcc gcagcgcctc
      241 gcgaaactcg cgctcggctt cgtcgaagcg gcgcgtcatc gcgtacgccg accccagcgt
      301 cacgtgcccg ttcgcgtagg cgggctcggt cttgaccgcc tgttccgcga aacggagccc
      361 ctcgtcgatg cgctgcgttt cgatcagcat gaccgcgagg ttgttcatgg cgtagaagtc
      421 atccggcttg agggcgatgc acttgcgcag cgccgcttcg gcctcgtcgt agcgcttgag
      481 cttgccgtag cacaccgcga ggttgttgta cgcgccgtgg aagttgggcc gcatcgcgat
      541 cgcctctttg taggccgcga ttgccttctc gtactccccg cgctcttgat aacgctgccc
      601 gaggttgttg tgcgcgtcgg cgttgtcggg cgcgaggtag cccagcgcct ccagcttctt
      661 catggtctcc gcgtccgcgg acgacgccgc cgcgtcgctg tgcgcctcgc cgcgcggacc
      721 gtccagcgtc gcgacggtct cgctcgagaa cgtcgccacc accgcgtcgt cgaaggccga
      781 cgccagcggc ttgccgggca tgtcgcccgc gcgcgggagg ccttgcagcg cgagaatcgt
      841 cggcgcaacg tcgaggatgg acgcgccgtc gatggtcgcg cccctcttca ctcccgcccc
      901 gaagaacgcg acgatgccgt ctatgcggtg ccaaagcgcg gcgttgcccg cccagatctc
      961 ggggcggttc ttcaagcgcg actctccgct cttgaatccg tggtccgaga ccaccatgag
     1021 cacggtgcgc tcatcgatct tcgacatgat ctctcccaga atctcgtcct gcgcgacgta
     1081 ggtctcatca atggcgcgcg agtagcgcgc gtactccttc tccggaacgt cgggcatgcg
     1141 gggtgaagcg tgcagcatga agagatgact catcgcgtcc acccactcga aatacaccgc
     1201 cagaacgtcg ggctggtcgt tctgccaaag ggtcgtcgcg atgtttcgga aggtcaacgt
     1261 cgacgcgtac aagtggatca ggttgttgac cggatccttg gggtcgaagg agccgcggtg
     1321 tttcgacagc tcggcgtggg gtacctgaac gaaccggccc acctggtcgt cggtgatcgc
     1381 atccgcgtgc acgaccatgc ccgcgagctc gccggcgcgc gcgcgcggat ggacactgga
     1441 cggcgccgag gcgcgcgcgc tgtcaccccg ggaagccgtg ggcgcgtacg cgacataccc
     1501 gaacttgtcg gtgaccatga cgccgtcgat cgcttcggcg ggatcggtgg cgagccagcc
     1561 cacgacgacg acgcggcggt cgtggcgcga gagcatgttc cagaacgcgt cgaccttgcg
     1621 gtacagccgg ctgatgggga cgcgcgcgcc ggttttggga tcgacgacgg taaaattgag
     1681 aacgccgtgg tcttcggggt agcgtcccgt cgccatggtg gtccacaaca gcggcgagag
     1741 catcggctct tcggagcgaa gcgggcccgt ggcaccttcg cgcacgaggc gagcgaagtt
     1801 cgggagcctg cccgcgtcga tgagcggctt caggttctgc cagtcgcccc cgtcgacgcc
     1861 cacgatgacg agccggcgcg gcgccttcga ctccacgcgc atggacgcgt ccgcgcgcgc
     1921 gcgtgcgcca cggcggacct cgacgcccag ggcctgcatt tcgtcgcgca cctggtcgac
     1981 aaccgcggcg tcgaggtcgc tcgcttcggc cgcggtggtg cggctggcgg cttcgatctc
     2041 ggccaccgac accaccaggt tcgagaacgc gtcctcgaag tccttcgaaa actgtcggta
     2101 gaggacttcc gaactgccgg gcgggaacgc gagatcgaac tcgtacgcga cggcgagcga
     2161 atcgcccatc tgaaagatga cgtgaaacga atcgctctcg ggaacgcgaa aggaatgctc
     2221 gccggaggga tagcgtatca gcgtcgttcc gggtcccacc cagtgccaac cgctcgcgag
     2281 caccgccacg ccgccgtcgc gccccacgcg cacggcctcg cggtcggcgg ggacgcgctt
     2341 caagccggcg gcggcgccca acaggagcag cgccgcgatg gcgatcacta cgagacgaat
     2401 acgcttcatc gggatgggag ggctagtata atgcatcgac catggattcc gcgatacccg
     2461 tgcgagtgcc cgtccctccg tcggtggtcg tcgtcgtgct cacgtggaac ggccgcgcgc
     2521 tcacgatgga ctgcctgcgg tcgctcgagg ccgtcgccac gcccaacgtg cgcgtgatgg
     2581 tggtcgacaa cgcctccacc gacgggaccg ccgcggccat ccgcgagcgc tacggcgagc
     2641 gggtcgaggt ggtcgagaac gcctccaatc tgggctatgc ggggggcaac aacgtcggca
     2701 tccggcgcgc gctcgaccag ggggcccgct tcgtcctgct cctcaacaac gacaccaccg
     2761 tggcacccga cttcgtcgac cagctcttgg gcgcgacgct cgacccggcg atcgggatcg
     2821 ccgcgcccaa gatctacttc gccgagcccc cggaccgcat ctggtatgcg ggcggcgaga
     2881 tctccttgtg gcgcggtgcc gcgcgtcacg ccgggatccg cgagcacgac cgcggccagc
     2941 acgacaccgc gcgcgacgtc gactacgcga gcgggtgcgc gctcctcgcg cgccgcgagg
     3001 tattcgagcg cgtggggctt ctcgacgaat cctatcgtgc gtacttcgag gacgccgacc
     3061 tgtgcctgcg tgcggcgcgc gccggttttc gggtccgcta cgtaccgacc gcccaggtgt
     3121 ggcaccgcat ctccgcgagc acgggcggcc aggtctcgcg ggccaaggtt cgccgcaagc
     3181 tcgagagcgc gtggcgcttc tttcgcgccc acgcgcggcc ctatcactgg atcaccattc
     3241 ctttattttt cgccctcgat gtggtacgaa ttaccggcct ggttctggtc ggccgcatcc
     3301 gcgatgccga cccactctag cccaacgaac accttgaacc atcggacggt tccttcgcgg
     3361 tgaaggtcgc gatcttcggg gtcggcggcc aactcggtcg cgccgttgcc gccacgctgg
     3421 cgacgacgca cgacgtcgtt tccatcgacc acgaccgcgc cgacgtccgc cacgccgtcg
     3481 cggtcgccga cgtggtgcgc gcggcccagc ccgagtgggt gctcaactgc gcggccatga
     3541 cgcacgtcga ccgctgcgag aacgacccgc tggccgcctt cgaggtcaac gcgctcgggg
     3601 cgcgcaacgt ggcccgggcc gccgccgccg ccggagcacg ccttctgcac gtcagcaccg
     3661 actacgtatt cgacggcgaa aagccgtcac cctatggcga agacgacgcc gttcgaccca
     3721 tcaacgccta cggcgtcagc aagctctcgg gcgagtggtt cgtgcgcgcg gactgccccg
     3781 cccacgcgat cgtgcgcacg agcgggctct acgggtggta cgtgtgtcgc ggcaaggggg
     3841 cgaacttcgc ggagaccatc ctcgcgcgcg cgcgccaggg ccagccgctg cgcgtcgtct
     3901 ccgacgagcg cttgacgccc acgttcaccg aggacctggc ggcgcagttg cgagcaatca
     3961 tcgagggcaa cgtgcccagc ggcgtctacc acgcaaccaa cgccggcgcg tgctcgtggt
     4021 tcgatttcgc gagcgagctc gtgcgcctgg ccggcgtacc ggcgtcgatc cagcccattt
     4081 ccgcgcggga gtggaagtcg cccacgcgac gccccgccaa ctcggtgctg gagaaccgcg
     4141 ccctcgcgaa gctcgggctc gaccgcatgc cggactggcg cgacgcgctc gcgcgctacc
     4201 tcgcggcccg cgacgacaag gccggcccgg ccgcgtgatc gactagaaaa agaccgcgca
     4261 ggcgagcgcg gccgcaccga gggccagcac gatggccacg aacgcgatcg cgaccgagag
     4321 gccgagcgcg gccagcggaa tcgcgaacag accgagtacg agtcctttga agacggcgaa
     4381 agccaatttg aacggcaaga gaaccaaccc cacgaggatg tggagcatga ccgcacccac
     4441 gaagagaccc acgaggctga ccaagccgat cacgatgaac cattcgatca cgagacacct
     4501 cccgacggga ccggtcgcgc acacgcgcgg cttcgtcccc ggttacccgg gccgcggccg
     4561 ttcattcgaa aacggcgcga agccacgacg gcggtgggag ccattcgcgg ccatcgccgc
     4621 gctcgtactg gtcgcgtccg cggccaacgc cgcggatccc ccgcgcgtga tgactccgga
     4681 cccgctgcct tacgtctcgc cgagccgcgc ctacgacacc gagcacacct ccctcgacct
     4741 cgacatcgac cttcaggcgc gtcgcgtcgc cggtcacgtc acgcacaccg tccgcgcgct
     4801 gcgcagcgac ctctccgagg tgcgcatgaa ctgcgtcgag ctcatgatcg actcggtcac
     4861 cgtcgacggg cggcgcgcgc gcttcgagta cccggtgtcg gcgggccaga gcacgtcgtg
     4921 gctcgcgggc gcgtcggccg gaaccgccga cgaccaactg gccgtgatct gcgatccccc
     4981 gctggcacgc ggcgcccaag cgcgcgtgag cgtctactat tcgggtactc ccgagatcgg
     5041 cctctattgg attcgccccg agaaagggat ccccgacaag cgctacgagg tgtggtcgca
     5101 gggcgaaggc gaagacaacc gttactggat cccgtgtaac gactacccca acgacaaggc
     5161 caccttcgaa ggacgattcc gagtggccaa gggttacacc gccatctcga acgggaccct
     5221 gatggggacg cgcgaagtgg gaggaaagac cgagtttcac tggaagctcg aagagccgca
     5281 ggtgagctat ctcatcatgc tcgcggtcgc cgagtacaag gttctcgagc agaaggcggg
     5341 caccatcccg ctgccctacg tcgtcccgcc cggcaccgac gacgccacca tcctgcgcgg
     5401 ctacggactc acgcccgaca tgatggacta cttcgagaag gagatcggca tcccgtatcc
     5461 gttctcgaag tacgcgcagg tggtcgtgca ggactacatc tacggcggca tggaaaacac
     5521 caccgcgacc gtgatgaaca tgcgcacgct ctacgacgag cgcgccgagc tcacgcgtac
     5581 cgagcagaac ctggtcgcgc acgagctcgc ccaccaatgg tggggcgaca tggtgacgtg
     5641 ccgcgaatgg agccacatgt ggctcaacga gggcttcgcg acctattacg cctacctata
     5701 caaggagcac cgcgacggcg acgacgcctt tcgctaccag atgcgccagg cgcataacga
     5761 cgtaatcaaa gccgacgacg ccgagccgcg ccccatggtg gtcgacttct tcaaccgcac
     5821 cgatgcgcgc aacaacgcgc tcgtgtacgt caagggcgct agcctcttgc acatgctgcg
     5881 cgcctatctg ggtgacgatc tctaccgaga gacgattcgg cactacgggg aggaaaacaa
     5941 gcacgacgtg gtcgagaccc aggacctgat gcgggcggtg cgcgaggtga cgggcgagaa
     6001 tctggactgg ttcttcgagc aatgggcctt cttggccgga catcccaagt tcaaggtgac
     6061 gaagagctgg gaccgggaca cgcgcgtgct gacactcaag gtcgaacaaa cccaaaagac
     6121 ggtcggcgtg gtgcccgtgt ttcgcgtgcc cgtggacgtc gagatcgggt ggagcggcgg
     6181 gagcgccctc caccgcgtca tgatcgagga agcctccgcg agctattact ttcccgtccc
     6241 cgaggaaccc aggtccgtcg tcttcgacaa gggcgattgg atattgaaga cggtcgactt
     6301 tcccaagctc gcgggcgagc tcgtccacga gctcgagaac ggcgatttca tgacccgcgt
     6361 cgtcgcggcc gaagcgctgg gggccaaagg ctcggacgcg cgcgtggtac cggcgctgcg
     6421 tgccgtggtg ctgggcgacg cgttctgggg actccggcgc gaatcggcgc tttcgctggg
     6481 caagatcggc actgacgcgg ccctctccgc tctcgtcgaa gggaccgcgg tcgaggacgc
     6541 gcgcgtgcgg ctggcgtgcg ccgaagcgct gggcggctac tatcggaacg ccaaggcggc
     6601 ggctcagctc gagaagctgg tcggaaacga tcgcgcatac ggcgtgcgcg ccgcagcact
     6661 gaccgccctg gtgaaagtgc gcggcgagcg tgcggcccgc gtctgcgccg atgcgctcaa
     6721 gcaaacgtcg gaccaggaca tcgtccgcaa cgcggcgctc aacgggctcg ccgatttgaa
     6781 cgacaccgcg gccctcgatc gcatcaggcc gtactgcgca agcggcaacc cgcgctggta
     6841 ccggcacacc gccatcgccg cgtacggacg actcgcaaag cagctcgaca aggagtccga
     6901 ccggcgccgc gccgcggagt ctttgtatcc cgcgctggac gactggcacc tgcgcacgcg
     6961 tgaagcggcc atcgacgcgc tcgcggccat cggggaaccc tcgtcggtgg agccgctgcg
     7021 gcgcgtggcc gccaacgacc gcctggccgc ggtgcgcgag cgcgcggcca aggcggccga
     7081 tcgcatcggc gccgagtcgg ccaaaacgac cgcgcgcaac aacgcggccg acatcgaaga
     7141 gctaaaacgc cgcttggagt cggtggagcg cgaactggag gccgccaaga agaagctggc
     7201 agcacccgcc tccgaagccg ccgggagcga ctagtccatt gtcaaataac acgttagaca
     7261 cctggaaaag taccggattg agtttccggt tgattttgat aaacgtttca catagtctgt
     7321 tggcggttgc tcgagggaac gacgcacggt acagtcgagc cttcttgagg gaatgggccc
     7381 ctcgacgcga ccggcgcgac gaagtgcgct cgtgaaatcg gacaaggacc ggtattcgtg
     7441 ggcccgccag gcggggatgc tgagcacgat ccccttcctg atggtggttc ccccgatcgc
     7501 cggactccta atcggccggt tcatcgaccg aaagctcgac actcaacccg cattcaccat
     7561 cgttttcctg atcctgggtt tcgccgcggg cgttcgcgag gtcgcgatcg tcctgaagcg
     7621 ggcgaacgcc cgggacgacg acggagacaa gcgtgatcga agtgcgccct agcggtgcgg
     7681 tgtggtctgc gatcgacggc gactttctgc gccgcgttcg cacgtcgtcg atcgccttcg
     7741 gatgcgtgct cgcgataccg ctggccacga aattcgggtg gttggccgcg ggcgcgtgga
     7801 tcgcgggcgg cgcgtggtcg atcgtcaatc tggcggcgat cgcgtcggtg atgcgcaagg
     7861 tcttgacgct ggagccgcgc gaccgcggcg cgatcgtgaa ggccctcgcc atcaagttcc
     7921 cggtgctgta cgcgatcggt ttcggattgc tcgccgcggg gctgccggtg atgtggctct
     7981 tggccggatt cgcgtggccg ctgttggtcg cggtgctcaa ggcggctggc cgcagttatc
     8041 ttcgcctcga cgagacggcg gacgcaccga gacaaggaaa cacacgtgtt ctctcctagc
     8101 ctgacacgca tgttcgacgg actggccgcg atgcgattgg ccgcggaagg ccacgccgac
     8161 agcgcacacg ccgtcgtctc acacgatgcg tcgcacgccg ccgcggccca cgccgaagag
     8221 catgcggtcc acgagctccc caactggatc acgctcatta ccaagttcat gcaggattct
     8281 ccggcgaagg agttcctgca ccaatgggaa ggcatgatct gggccctcgc catcgctacc
     8341 ttgatcatcg tgatcgcgcg cctggcggcg cgcaacccgc agatggtgcc gggacgcctg
     8401 cagaacatcg tcgagatggt ggtcgaagga ctctccaact tcatcgtcgg ggtcttgggt
     8461 cccgaagggc gccgctacgt cccgttcctc ggcacgctgt tcatctacat tctgtccatg
     8521 aacctggccg gcgtgtttcc gctgggcaag tcgcccaccg cgagcccgaa cacaaccgtc
     8581 gcgctggcgc tgtgcgtctt cgtctacgtg cacttcatcg ggctcacccg caacgggccg
     8641 gtcggttaca tgaagcactt ggccggcaat cccaggtcgc cggtggaatg ggcgttcgtc
     8701 ccgctcatgc tgcccatcca cgtgatcggc gagatcgcgc gcccggtgtc gctgtcgctg
     8761 cgactcttcg gtaacgtcac cggtgaggac gtgctgctgt tcgcgttttg cagcctgggc
     8821 gtcaccacgc tcgcgttcgt cgattcaccg gtcgggattc cgttccaggt accgttcatc
     8881 ttgctcgcgt tcatgaccag ctttatccag gcgctcgtgt tcacactgtt gagcacgatc
     8941 tacattttgc tgtggctgcc ccacgacgaa cacgccgagg agcaccacta agtcgtttcg
     9001 tagcgtcgtt tcgaaaacca acgaggagga aagaacaatg gatacttcgt ccgcattgag
     9061 tttggcgctt cccatcgggc tcggcctggc ggccatcggc tcgggcatcg gattccaggg
     9121 tgcgatcggc aaggccatgg aagccatcgg tcgccagccg gaggcgtcgg gcaagattca
     9181 gaccgcgatg atcatcggcg ccggcctgat cgaagctctg accatctacg cgttcgtgac
     9241 cgtgttcttg ttgatgggca agatccagta acgtgatgcc aatacgcgac ggtgtgcgtc
     9301 ccgcggcggg tgtcgccgat ggggcccccg ccgcgccagg cggcggggtg atcggtgaca
     9361 ggacccgccg cgcagcgttc ggtctgatct aggagagtaa gatggaactt cagtggaaag
     9421 cattactcac ccaggtggtg ggctttctcg tcgtcctgtg gctcttgcgg aagtacgcgt
     9481 ggggcaagct cctcgcgttc accgagaagc gccgcgagac catcgcgaac gagttcgcga
     9541 acatcgagaa agcgaaagtg gacgcggacg cgctgcgccg gcgcttcgaa gaagagctag
     9601 cgggcatcga gaatacgcgc cgcacgcgca tccaggacgc cgcgcacgag gccaacgaga
     9661 tcgcgagcca gatccgcgaa gaggcgcgtc gcgagaccgt ggacttgcgc gtcaaggcca
     9721 accaggacat cgcgatggag atggacaagg ccaacgccgc gttccgcgac caaatggtca
     9781 acgcggtgat cgcggctacg gacaagctca ttcgcctgcg cctggacgac gcccagcacc
     9841 gccgcctgat caacgagttc ctggacgaag tcaccacccg gccggcgagc cggtaggaga
     9901 accatggcca ccgccggtgt cggaaaacga tacgcgatcg cgctgttcaa cgcggcccag
     9961 gccgaggacg tcctcgacca ggtgcacggc gacgtgacca gcttcggcaa gttgctcgcg
    10021 agcgaagaag gcttccgccg cttcctgctc tcggggcgcg tgaccggcga cgagaagcag
    10081 ggactgatcg tgaaggcgct gggcgagcgc gcctccgggc tcttcgtcaa gttcgtgctg
    10141 ctcctgatcg acaagaagcg caccgcggcc tacgacgaca tcgcgcgcgc gttcgtgtcg
    10201 ctctacgaag agtacaaggg cgtggtcgaa gtgcgcgtcg tgaccgcgat acccctcgac
    10261 acggagctgg agcgcaaggc caaggacgtc gtcgagcggc gcaccggcaa gaaggtcaag
    10321 ctcgagaagc gcatcgagcc gggcatcgtc ggcgggatga tcatgttcat cgagaaccag
    10381 gtgttcgacg gcagcattcg aaaccgactc ggcgacttgg gcacggcgct gctcgccacc
    10441 cgagtgcacg tggtcgatca atcttagctt aggagagaag caatggcgtt tcgacccgaa
    10501 gaagtcagtt ccatcatcca agctcaactc cagaagacga gtctcgagct cgagatggag
    10561 tcggtcggta ccattctgca ggtgggcgac ggtattgcgc gcatttgggg tctcgaagac
    10621 gccatgtcgg gcgagctgct cgagttcccc aacggcggcg tcggcatggt catgaacttg
    10681 gaagccgaca acgtgggtgc ggtgctgttc ggctccgaca ccggcatcaa ggaaggcgac
    10741 gccgtcaagc gcacgggtcg cgtggcccag gtcccggtgg gcgaagcgct gctgggccga
    10801 gtggtcaact cgctggggca gcccatcgac ggcaagggtc ccatcgtcac cgacacctac
    10861 aacccgatgg agccgctggt cccgaacgtc gtgcagcggc agccggtgaa agagccgctg
    10921 caaaccgggc tcaaggccgt cgactcgatg attcccatcg gccgcggcca gcgcgagctg
    10981 atcatcggcg accgccagac cggcaagacc gccatttgca tcgacaccat catcaatcag
    11041 aagggcaccg gcgtgaagtg catctacgtc gcggtcggcc agaaggaatc gaccgtcgcg
    11101 caaatcgtct ccaagctgga gcagcacggt gccatggaat acaccaccat cgtcagcgcc
    11161 accgcgagcg agtcggccgc gttgcagatg ctggcgccgt acgccggttg tgccatgggc
    11221 gagttcttcc gcgaccgcgg tggccacgcg gtgtgcttct acgacgatct ttccaagcac
    11281 gctcaggcct accgccaaat gtcgctcctg ctccgccgtc cgccgggccg cgaggcgtac
    11341 ccgggcgacg tgttctatct gcactcgcgc ttgctcgagc gcgcggccaa gctcaacgat
    11401 aacctggggg gcggctcgct gacggcgctg ccgatcatcg agacccagct gggcgacgtc
    11461 accgcgtaca tcccgaccaa cgtgatctcg atcaccgacg ggcagatctt cctggaaggc
    11521 gacctgttct actcgggcgt tcgcccggcc atcaatgtcg gcatctcggt gtcccgtgtg
    11581 ggctcgaacg cgcagaccaa gatgatgaag caggtcgcgg gccgcttgcg cctggccctc
    11641 gcgcagtacc gcgagctggc cgcgttcgcg cgtttcggct ccgacctcga caagtcgacg
    11701 ctcgcgcagc tcacgcgcgg cgagaagatg gtcgagatct tgaagcagga cgaaaacgtg
    11761 ccgctcgcga ccgagaagca ggtactgctc atcttcgcgg ccaacgaggg cctgctcgac
    11821 gacctcccga ccgagagcct gaagcgcttc gagccggagt tcttcgcctt catggacagc
    11881 aagtacccgg acgtcgttcg ccagctgcgg gagaagaagg cgttcgacaa gcaagtcgag
    11941 gacgaagcgc gcaaggcggc cggagagttc aaggcggcct tcgtgaagtc actcccggcc
    12001 aagaaggcct aacggacacg acccatggcg accattcgcg acctcaagaa ccgcattcgc
    12061 gcggtcgaga acatccgcaa gattacgcgc gcgatgcagc tcgtggcggc ggccaagctc
    12121 aaccgcgccc agcagcgcgc gcacgcggtg cgtcccttcg cggacgagct ggatcgcgtg
    12181 ctgggcggtc tggccggcgg cggcatcgag gaatcggccg acgtggccat cgagttcgcg
    12241 tacgcggacg gggttgcgcc ggtcaagacg acgctcgggc ggtttttttc gacgcacgaa
    12301 gtgaagaagc ccggcctggt gctgttcacc gccgaccgcg gcttgagcgg cgccttcaat
    12361 accaacttga tccgcgccgc gcagacgttc ctgcgcgagc acgcccacca ggacgtcaag
    12421 ctcatcacca tcggccgcaa ggggtacggg ttcttccgcc gccgttcgac ccccatcttg
    12481 tacacgagga cgggcttgag cgatcgcttg ctcctgcccg agatcaagga agtcactggc
    12541 aagctgctcg agctttacgt caacgacgaa gtcgacgcgc tctatttcat ctatgcgaag
    12601 tcgctgcgcg cttcgctgta tcgcgtgacc acgcagcagt tcttggccat cccggccgtc
    12661 gcgggcgcca cctccgacga caactacctg atcgagccgg ggcgcgacga attgttcgcc
    12721 gacctgatcc cgttgtacgc aacctcgcgc gtgttctccg tgctggccga ttcgttcgcg
    12781 tc
//
LOCUS       DASPGK010000023        14107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_241155, whole genome shotgun sequence.
ACCESSION   DASPGK010000023 DASPGK010000000
VERSION     DASPGK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241155"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VEC56_00565"
     CDS             <1..331
                     /locus_tag="VEC56_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12671.1"
                     /translation="DGFEVTGGATAIGDETPKQFALRPNYPNPFNPATTIRFDVATRS
                     HVTLRVYDVSGAIVRTLVDETKGAGAHVVEWNGRNDQGDPAGSGVYFYRLTAPGFSDV
                     RKMTLIK"
     gene            333..1526
                     /locus_tag="VEC56_00570"
     CDS             333..1526
                     /locus_tag="VEC56_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HXV12672.1"
                     /translation="MYLYQTEARYFAQVPDGGEDVAAQELTALGAQEVKAGYRGLYFG
                     AERRALYGIVYRALTITRVLAPLIRFQCHSPEYLYKRTQSIAWSDFLTPKQTFAVFAT
                     VSNSAIRHSQYAALTIKDAIVDQFRDKTGARPSVETIEPDLWINLYLHANKATLSIDA
                     SGGSMHRRGYRVESIEAPMQETVAAAMLAMSEWDGSTPLVDPMCGSGTILCEALMRYC
                     RVPAGYLRARFGFERLPDFDREGWREIKREADGAIRTLPKDLIRGSDASPEAVRAARA
                     NLARLPGGRVVAVEKRRFEDVERVEGAAIVTNPPYGLRIGKRDAMPEFVKRFGDFLKH
                     RCAGSSAFVYFGDRELLKSFGLRPTWKRPLASGGLDGRLVRVDLFAGKRDALKVARTP
                     DAVRE"
     gene            1596..3971
                     /locus_tag="VEC56_00575"
     CDS             1596..3971
                     /locus_tag="VEC56_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12673.1"
                     /translation="MHRETTVVNSALFVGLVFAFLGATTADAALRHGRPPMSSGEAKG
                     VTLLGETDFDCGTYKGNEVEHAWRKAVNDRVQSEVKTGRRIVQGGLDYVYDDVWIVED
                     DGTLTFSGTNAFDTNLSTFQFAPAGGGVYNVTEPAFSFDAALGPAIAPGDDGAILVAL
                     PFSFPYGGGSWTQMYVGANGILSFGAHPNPSGFFDPADFYSTTPKIAGYYLDLNPAAG
                     GTVNTKSEPTKYTVTWNTVPEYGTSNFNTIQIVLYASGTFTSTYNGIASTVAASPIVL
                     GFHPGGAPALETISFSAGLPHASGAGAAVYEEYFSYASPLVNEVALMQRFYSQFPDEF
                     FQLVFFTNFTQTMSGFANELNIKNDVTGIGLGIFDSSGQYGSSGVLESRCNMNRLSVW
                     NFDPYFRFFSKGNNFLTIMGQEAGHRWGAFTYFDSGSGPSNLILGRSDAHWSYYVDVD
                     HSSLEGGNWISTGGSNYQCPTQIDFFSQLDEYTFGLRTPDEVKDFFYISSASNNLPGN
                     RSVGTPLLGATASGTLTPVTVEHIIAANGARTPTEPNENKDLRQGFIFLLQAGSSPSQ
                     AELDKIAGFRRAWEEYFERSCDGRLTCNTSITSNYDVAVICGNVRERWTGDLIPAFTA
                     RSIERSFVQDVPDDGRYTFRYQASATSGPSEGATIVFTAAGYEPDTLEVSLTYGTTTC
                     IDVELMVDQTTPTDRVPVTTELFANHPNPFNPSTTIRFNLGAAGPVQLVVFDASGRRV
                     RTLLNETRSVGEHAVTFDGRDERGNVLASGVYFYRMEAGGVVQTRKMVLLK"
     gene            complement(4084..4557)
                     /locus_tag="VEC56_00580"
     CDS             complement(4084..4557)
                     /locus_tag="VEC56_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12674.1"
                     /translation="MSRSNHKKINAALVIATVCICLWPGPAPANDMGVHMGYYFDSEA
                     ISLGFGLLTSMSSSQPRWFFNPNAEMIVGDGRDMAAFNFDFHYDFATDSDLTFWAGAG
                     PGLYLIERPFDDDLEVGLNVLAGFGAQTGSVRPFVQGKAVVMDDAEASLAVGLRF"
     gene            complement(4774..5910)
                     /locus_tag="VEC56_00585"
     CDS             complement(4774..5910)
                     /locus_tag="VEC56_00585"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXV12675.1"
                     /translation="MPQPSYKTDFWKGLSFKARAMLSLGIGSTFATIGIFQDFWAPGA
                     PPWYVVALHCVIAASISLSWAYTFMWSKKFIPAIVVTTLASIVLGMWTSFRFSSSAVT
                     DIGAWQTRASIEAFACMILIALGYIFFVRFIDTEGTEQLRLRTEVNLAREIHEVLVPP
                     VHAIHGRLEIFGRSIASSEVGGDLLDVFEGEHGVVVTVADVSGHGVAAGTMMAMMKSA
                     ARVKLMDGAKLDALVYDLNRVMFQLKGDGMFATISALRFHRDGSVEVAVAGHLPLLRV
                     RGNDGEVDVLPNEHVPLGILEDASFWSRDLRGESGDLFVLITDGLTEVENAKGEELGW
                     ERIRDITASLRTRPLPDIYTAVMDAVTSHGPQADDQTLVLIRVG"
     gene            5954..6373
                     /gene="arfB"
                     /locus_tag="VEC56_00590"
     CDS             5954..6373
                     /gene="arfB"
                     /locus_tag="VEC56_00590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXV12676.1"
                     /translation="MILVTPTISLDESELEVSFVRSSGPGGQNVNKVATAVQLRFDVA
                     RSPSLPDDVRARLLARLGSKLTKDGVLILSARRFRTQERNRADAVERLVELIRASATP
                     PKRRRKTKPTRASKQQRVEEKRRRSATKRMRTEPVDE"
     gene            6699..7613
                     /locus_tag="VEC56_00595"
     CDS             6699..7613
                     /locus_tag="VEC56_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12677.1"
                     /translation="MIGSPEGDDGELFGRISDIVVGPHGEVYVADLGYQRVQKYDSTG
                     QHVGVVGGQGEGPGLYYDPCAVALDSKANVYVASRGRISIFDGHGVFVQELRTNLSGF
                     VRKIRVNTDNVVFVSCFDLMSQQIVHRFPLTDGTRGGSFCDSYAVDSDEDTREEMMIA
                     GGSIDIRNDGTILYTQMTPYEIRLFSPEGVLLSAIYRANDFMTPPRVEYLPGGGVTLH
                     AYPMSVSIIVLGDGRFVNAVIRPVSAEESETILDVYEGAGVLIGTTRLAGALGLKCRD
                     ERGRLYASERGDFPKVVKYKVHFEGGRK"
     gene            7873..8289
                     /locus_tag="VEC56_00600"
     CDS             7873..8289
                     /locus_tag="VEC56_00600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV12678.1"
                     /translation="MLRSFKRDLSSLEPLFEFAGDFARTHRLDDAIVFAMNLAVEELF
                     TNMVKYGGGDDMVSVGLDVRGDDLVIELVHAGANPFDPGAAAPVDVTRTLDERRPGGV
                     GLHLVKSVMDDFRYEHKNGDARITVTKHLGPRPEGA"
     gene            8290..8574
                     /locus_tag="VEC56_00605"
     CDS             8290..8574
                     /locus_tag="VEC56_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV12679.1"
                     /translation="MFDIRRGTNGVILLTGRFDAAHVERVRDELNSVTQSCTVDFTDL
                     DYISSAGMGVLLGTQKRLAGSGHSLKLVNLNRHIREIFRIAGFDNVFFIE"
     gene            8621..9166
                     /locus_tag="VEC56_00610"
     CDS             8621..9166
                     /locus_tag="VEC56_00610"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV12680.1"
                     /translation="MRDHADAELVRACLSGNQKAFEVLVRKYEKPIYNLALRMLKDRD
                     DAADVAQTTFVKAYQKLDSFDEAHEFFSWIYRIAINESINFQKRTKRLDEYESGVTAA
                     LSATQEQAHGAEVLRDEMIDAIERLTLDYQMVIVLRHFHDFSYEEMADVLSIPEKTVK
                     SRLFTARQQLKDILSAKGITR"
     gene            9163..9807
                     /locus_tag="VEC56_00615"
     CDS             9163..9807
                     /locus_tag="VEC56_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12681.1"
                     /translation="MIDPKHIELMNRVLDGAATEPERADLDRILTSSPEAHAHYDHLR
                     RLVSRLDSVPMVDPPAELYPRVVSAVDASGIRHARSHEEHGFWAWLRHAFAPPGLRYA
                     STFGLGLAAGAFVLALVRPGTPVDPSQVSGTLANPGATGSMPVAVPEAGVWGSVAVVD
                     RGPVTQVHLQVDGQGGTEWVFDVPNRAEPSQSVVLRVVKSGETVFEGAVRPVEP"
     gene            9935..11056
                     /locus_tag="VEC56_00620"
     CDS             9935..11056
                     /locus_tag="VEC56_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12682.1"
                     /translation="MSRKSTTLLITTSVVIIAAVAFFTSNRNNPTSDQVAGAIGTVDK
                     YRSEQITPSDVDIQGQSPTSDDVFATWLNDTATLEELAGFMGRLSPQERLKFFDRATP
                     EMAAMIVERATVTEKASLFNRATPEERAAMVARMTPDERRKYLGKANVADLAAFEKMS
                     VSERATALDRLIPAEKAAIFERAIPSEKAVALSRCDVTERATILDRATVAERAQVLGR
                     ATVAERASLYERCTPAERAAVVGRMSIEGKAAMLDRAAVAEKSAIFERATPSEKVTMY
                     DNLTVGEKATVLEKTNITIESFAKMPLEERASVLSRATPEGRAQLLDRATVQAKAAIF
                     ERAPENERAQIFERAIVAERGTITNEKATPTMEKASATN"
     gene            11173..>14107
                     /locus_tag="VEC56_00625"
     CDS             11173..>14107
                     /locus_tag="VEC56_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12683.1"
                     /translation="MQIQAPGNLRGFVAAPLFAFAIVLALVPRGAHAQLDFQSLETST
                     MRVVYYDPEHAYIIPHLTRCFENSMAFYYDFVGYTPYEHVTILLQDFDDYGYAGTSTI
                     PNNYLTLGIEPFEYVYETCPTNERFNWVMSHELWHVVASEKASHTDRVFRGLFQGKVM
                     ADANNPESMIYSYLCSPRRFAPRWYHEGSAVFIETWLAGGIGRALGGYDEMAFRAMVE
                     SGAYFYDLVGLESEGTTSDFQIGQNSYLYGTRFMSYIALQYGPDKVVEWVNRRDDSKA
                     YYASEFKHLFGQKIDDEWKKWIAFEHEFQKQNLARIREYPVTQERVACEKALGSVSRG
                     YVDKQTGKLLVAVNYPAEFAHVASVDLATGRLEKIHDVQTPALYYVTSLAFDEAGRTL
                     FYTTNNGKHWRDLNAIDLRTRASRRLVTNCRIGDLAYCRADSTLWGVQHHNGFSTIVR
                     ISPPYDDWNKILPVMEFKYGTDVFDLDVSPDGTVLTASMLEINGAVKLVRFDVPDLMK
                     GQAGYSVLHEFANTAPANFVFSEDGAHLYGTSYQTGVSNVFRWSFAEQKMECVTNAEI
                     GYFRPIPMGDSLVVFSYTSDGFRPAVIADTTLQDVNAIRYLGQAVVDEWPVVKDWKLG
                     SPREVNLDSVTTYQGAYHKFRSMTTASLYPIVEAYKAYTSIGLRWNFMDPLGMHGGEL
                     KVGVTPTSSLPDDELVHAGFTYRHFPWTLKAAYNKADFYDFFGPTKTSRKGYGVGLIR
                     SDFLIADKPRFLEYTLDVAYYGGLERLPYNQNVLATFDSYASASASLDYKRTQSSIGA
                     ADSEKGVRWSLGVEGILVNQDFYTKGIAELDFGFLTPIDHSSIWLRGAGGYSRGDRDD
                     SFASFYFGGFGNNYVDHQEVKRYRDYDRFPGVEIDEIAGTNFARATVEWTLPPVRFRR
                     IGFQQLYCTYARTAVFASGIATNLDDDAFRREVGNVGGQVDFRLVIFSGLESTLSFGY
                     AAAKEE"
BASE COUNT         2643 a         4511 c         4481 g         2472 t
ORIGIN      
        1 ggacggcttc gaagtcaccg gcggcgccac cgcgatcggc gacgagacgc cgaagcagtt
       61 cgcgctgcgg cccaattacc cgaacccgtt caacccggcc accacgattc gcttcgacgt
      121 ggcgacgcgt tcgcacgtga cgctgcgcgt gtacgacgtg agcggcgcga tcgtgcgcac
      181 gctggtcgac gagaccaagg gcgcgggcgc gcatgtcgtc gagtggaacg ggcgcaacga
      241 ccagggtgac ccggccggct cgggcgtgta tttctatcgt ctgaccgcgc ccggtttctc
      301 ggacgtgcgc aagatgacgc tgatcaagta agttgtatct ctatcaaacc gaagcgcgct
      361 acttcgcgca agtgcccgac ggcggcgagg acgtcgccgc gcaggagcta acggcgctcg
      421 gtgcccaaga ggtgaaggcc ggatatcgcg gcctgtactt cggcgccgag cgccgggcgc
      481 tttatgggat cgtgtaccgt gcgctaacca tcacgcgcgt gctggcgccg ctgatccgct
      541 tccagtgcca ctcgcccgag tatctctaca agcgcacgca atcgatcgcc tggtccgact
      601 tcttgacgcc gaagcagacc ttcgcggttt tcgcgacggt gtcgaacagc gccatccgcc
      661 attcgcagta tgcggcgctg accatcaagg acgcgatcgt cgaccagttc cgcgacaaga
      721 cgggcgcgcg ccccagcgtg gaaaccatcg agcccgacct ctggatcaac ctctacctcc
      781 acgccaacaa ggccacgctg agcatcgatg cctccggggg ctcgatgcac cgtcgcggtt
      841 atcgcgtcga gtccatcgaa gcgcccatgc aggagacggt tgcggccgcc atgctcgcga
      901 tgagcgagtg ggacggatcg acgccgctgg tcgaccccat gtgcgggtcg ggcaccatct
      961 tgtgcgaggc gctgatgcgc tactgccgcg tgccggcggg gtacctgcgc gcgcgcttcg
     1021 gtttcgagcg cttgccggac ttcgaccgcg agggatggcg tgagatcaag cgcgaggccg
     1081 acggcgccat ccgcacgttg ccgaaggatc tgatccgtgg cagcgacgcg tcgccggagg
     1141 cggtgcgcgc ggcgcgcgcc aatctcgcgc ggctgccggg aggccgtgtg gtcgcggtcg
     1201 agaagcgccg cttcgaggac gtcgagcgcg tcgagggggc cgccatcgtc accaacccgc
     1261 cctacggcct gcgcatcggt aagcgcgacg ccatgccgga gttcgtgaag cgcttcggag
     1321 atttcttgaa acaccgctgc gcggggtcga gcgccttcgt ttatttcggc gaccgcgagc
     1381 tcttgaaatc gttcgggctg cggccgacgt ggaagaggcc gctcgcgagc ggcggcctcg
     1441 acggccgcct ggtacgcgtg gacttgttcg cgggtaagcg cgacgcctta aaggttgcgc
     1501 gcacgccgga cgccgtgcga gaatagagac gccccatcct tgacccgtaa ccgattcgag
     1561 ccccaatcga tcccttccgc ggaggcatga ccgccatgca tcgagagacc accgtcgtca
     1621 atagcgcgct attcgtgggt ttggtgttcg cttttttggg agcgaccact gctgacgcgg
     1681 ccctgcgcca cggccgcccg ccgatgtcgt cgggcgaggc caaaggggtc acgctgctcg
     1741 gcgagaccga cttcgactgc gggacgtaca agggcaacga ggtcgagcac gcctggcgca
     1801 aggccgtcaa cgatcgcgtc caaagcgagg tcaagaccgg ccggcggatc gtccaaggcg
     1861 gactcgatta cgtttacgac gacgtatgga tcgtcgagga cgacgggact ctgaccttca
     1921 gcggcaccaa tgcgttcgac accaacctga gtacgttcca gttcgcgccc gcgggcggcg
     1981 gcgtctacaa cgtcaccgag cccgccttca gcttcgacgc cgcgttgggg cccgcgatcg
     2041 cgcccggaga cgacggcgcg attctggtag cgctgccatt cagcttccca tacggaggcg
     2101 gcagctggac gcagatgtac gtgggagcga acggcatcct gtcgttcggc gcacacccga
     2161 atccgtccgg cttcttcgac ccggcggatt tctacagcac gactccgaag atcgccggct
     2221 actacctgga tttgaacccc gccgccggcg gcaccgtgaa taccaagagc gagccgacca
     2281 agtacacggt cacgtggaac acggtcccgg agtacggcac cagcaacttc aacaccattc
     2341 agatcgtcct gtacgcgagc ggtacgttca cgtcgaccta caacggcatc gcgtcgacgg
     2401 tggccgcgag cccgatcgtg ctcggtttcc accccggggg cgccccggcg ctcgagacca
     2461 tctcgttctc cgcggggctg ccgcatgcca gcggcgccgg ggcggccgtg tacgaggagt
     2521 acttcagcta cgccagtccg ttggtcaacg aagtcgcgct gatgcagcgt ttctactcgc
     2581 agttccccga cgagttcttc cagctcgtgt tcttcaccaa cttcacgcag accatgtcgg
     2641 gcttcgcgaa cgagctcaac atcaagaacg acgtgaccgg catcgggctg ggcatcttcg
     2701 actcgagcgg ccagtacggc agcagcggcg tgctcgagag ccgctgcaac atgaaccggc
     2761 tctcggtgtg gaacttcgac ccgtacttcc ggttcttctc caaggggaac aattttctca
     2821 ccatcatggg gcaggaggcc gggcaccgct ggggtgcgtt cacctacttc gactcgggtt
     2881 ccggccccag caatctgatt ctggggcgaa gcgacgcgca ctggagctac tacgtcgacg
     2941 tcgaccacag ctcgctcgaa gggggcaact ggatcagcac gggcggcagc aactatcagt
     3001 gcccgacgca gatcgatttc ttctcccagc tcgacgagta cacgttcggc ctgcgcacgc
     3061 ccgacgaggt gaaggacttc ttctatattt cgtcggcgtc caataatctg ccgggcaatc
     3121 ggtccgtggg aactcccctg ttgggcgcga ccgcgtcggg gacgctgaca ccggtcaccg
     3181 tcgagcacat catcgcggcc aacggcgcgc gcacgcccac cgagcccaat gagaacaagg
     3241 acctgcgcca gggcttcatc ttcttgctcc aggccggatc gtcgccttcg caggccgagc
     3301 tggacaagat cgcgggcttt cggcgcgcct gggaagagta cttcgagcgc tcttgcgacg
     3361 gccgcctgac gtgcaacacg agcatcacgt cgaactacga cgtcgcggtg atctgcggaa
     3421 acgtgcgcga gcgctggacc ggcgatctga tcccggcgtt caccgcgcgc tcgatcgagc
     3481 gttcattcgt ccaagacgta cccgacgacg ggcgctacac cttccgctac caggcgagcg
     3541 cgacgtcggg tccgagcgag ggcgcgacca tcgtgttcac ggcggccggg tacgagcccg
     3601 acacgctcga ggtctcgctg acctacggca ctacgacgtg catcgacgtc gagctcatgg
     3661 tcgatcagac cactcccacc gaccgcgtgc cggtgacgac cgagttgttc gccaaccacc
     3721 ccaatccgtt caacccgtcc acgaccattc gcttcaacct gggcgcggcg gggccggtgc
     3781 aattggtcgt gttcgacgcg agcggccgtc gcgtgcgcac gctcttgaac gagactcgtt
     3841 cggtgggcga gcacgcggtg acgttcgacg ggcgcgacga gcgcggaaac gtcctggcga
     3901 gtggcgtcta cttctatcgc atggaagcgg gcggcgtcgt ccagaccagg aagatggtgc
     3961 tgctcaagta agcgatctcg atcgagcgga ggggccccgt cgccgccatt tgacaggtgg
     4021 tgcagacggg aagcgacgac tcggcccccc cgcgctcctt ctcccgctcg ccttcgctag
     4081 ccgctagaaa cggagtccaa ctgcgagcga cgcctcggcg tcgtccatca cgacggcctt
     4141 gccttgcacg aagggccgca cggaaccagt ctgcgcgccg aaccccgcga gcacgttgag
     4201 cccgacttcg aggtcgtcgt cgaaggggcg ctcgatcaag tacagccccg gtcccgctcc
     4261 ggcccagaac gtcaggtcgg aatcggtcgc gaagtcgtag tgaaaatcga aattgaacgc
     4321 ggccatgtcg cggccatcgc ccacgatcat ttccgcgttc gggttgaaga accaccgcgg
     4381 ctggctactc gacatcgacg tcaacagccc gaagcccaag ctgatggcct cgctgtcgaa
     4441 gtagtaaccc atgtggacac ccatatcatt ggcgggtgcc gggcccggcc acaggcagat
     4501 gcaaacggtc gcgattacaa gcgcagcgtt gatcttctta tggttggatc ggctcatcgc
     4561 tcctccttga agttgggaag accgcgtgaa cgacgccgcg gtcgacggcg gactacatgg
     4621 aaaagctggg ccgtcgaacc ggagcatagc cacttaaacg gagatgagat ttgtcttcat
     4681 gtcgcgcaag tgacgcgggg ataggaggta gggccgcgaa atcaggggcg cgggcgcgcc
     4741 cgtcgcacgt aagtctgctt acagttgcgt tgtctagccc actcgaatca gcaccagtgt
     4801 ctggtcgtcc gcttgcggtc cgtgcgacgt caccgcgtcc atcacggccg tgtagatgtc
     4861 ggggagcggg cgcgtgcgca acgacgcggt gatgtcgcgg atgcgctccc accccaactc
     4921 ctcgcccttc gcattttcga cttcggtgag cccgtcggtg atcaggacga agaggtctcc
     4981 cgattcgccg cgcagatcgc gcgaccagaa ggaggcgtcc tcgaggattc cgagcggcac
     5041 gtgctcgttg gggagcacgt cgacctcacc gtcgttgccg cgcacgcgca agagcggcag
     5101 gtgccccgcg accgccacct ccaccgagcc gtcgcggtgg aagcgcagcg ccgagatggt
     5161 cgcgaacatc ccgtcgccct tgagctggaa catgacgcgg ttcaagtcgt agacgagtgc
     5221 gtcgagcttg gccccgtcca tgagtttcac gcgggccgca ctcttcatca tcgccatcat
     5281 ggtgcccgcg gcgactccgt gcccggagac gtcggccacc gtgacgacca cgccgtgctc
     5341 gccctcgaac acgtcgagga ggtcgccgcc cacttcgctg ctcgcgatcg agcgcccgaa
     5401 gatctccagg cgcccgtgga tcgcgtgcac ggggggcacc agcacttcgt ggatctcgcg
     5461 cgccaggttg acctcggtgc gcagccgcag ctgctcggta ccctcggtgt cgatgaagcg
     5521 cacgaagaag atgtagccga gcgcgatcag gatcatgcac gcgaacgcct cgatcgacgc
     5581 gcgcgtctgc cacgcaccga tgtcggtgac ggccgagctc gagaaccgga acgaagtcca
     5641 catgcccagc acgatgctgg cgagtgtggt gaccacgatc gcgggaatga acttcttgct
     5701 ccacatgaac gtgtacgccc acgagagcga gatggaggcc gcgatgacgc agtggagcgc
     5761 gacgacgtac cagggcggag cgccgggggc ccagaagtcc tggaagatgc cgatggtcgc
     5821 gaaggtggac ccgatgccga gggacagcat cgcgcgcgcc ttgaacgaga ggcccttcca
     5881 gaagtcggtc ttatagctgg gttgcggcat ggcgttcgac tatcatacgc ggcgaagcgc
     5941 ggaagtgcca ccgatgatcc tcgtcacgcc caccatctcc ctcgacgaat ccgagctcga
     6001 ggtctcgttc gtgcgctcgt cagggccggg cgggcagaac gtcaataaag ttgcgaccgc
     6061 ggtgcagctg cgcttcgacg tcgcgcgctc gccctcgctc cccgacgacg tgcgcgcgcg
     6121 cttgctggca aggctcggga gcaaactcac caaggacgga gtgctgattc tcagcgcgcg
     6181 ccgctttcgc acccaggagc gcaaccgcgc cgacgccgtc gaacgcctgg tcgagttgat
     6241 ccgggcctcc gccacgcctc ccaagcgcag gaggaagacc aagccaacac gcgcctccaa
     6301 gcagcaacgc gtcgaggaga agcgccggcg ttctgcaacc aagcgcatgc gcaccgagcc
     6361 ggttgacgag tagcgcgacg cagtccaggc aattgctgtt agcggtcagg cattgacatg
     6421 tgccgtggcg gtgctacgat aacaccaccg ccggctagtc gcgctttgtt cggactcgaa
     6481 ctgggagaaa cgcgatgcgg tttcatcaac aattcgccgc tgttgcgtca gtttcttcgc
     6541 gcccaatctt cttccgcttt gtcgtaatct ctgtttgcat gacgggactt gcgtccacgt
     6601 ggaccaacgt cattgccggt caggagcctc aagtagtgcg gaacgacgat taccgaaagg
     6661 gctcgccgca ggtccaacta gtcctgagta gagaaggcgt gatcggttcc cccgaaggtg
     6721 atgacgggga gttgtttggt cgaataagcg acatcgttgt gggtccgcac ggagaagtct
     6781 acgtcgccga ccttggctat cagcgcgtcc aaaagtacga cagcaccggg caacacgtgg
     6841 gagttgtggg tggtcaagga gagggtcccg ggttgtacta cgacccgtgt gcggtcgctt
     6901 tggactcgaa ggccaacgtc tacgttgcga gtcgaggaag gataagcatt tttgacggtc
     6961 acggggtatt cgttcaagag ttgcgtacca atctttctgg ttttgttcgc aagatccgag
     7021 ttaacaccga caacgtcgtg tttgtatcgt gtttcgacct gatgagtcag cagattgttc
     7081 accggtttcc gttgaccgat ggtacgcgcg gtggatcatt ctgtgactca tatgcagttg
     7141 attcagatga ggatactcgc gaggaaatga tgatcgctgg tggctccatc gacattcgga
     7201 acgatggcac catactgtac acgcagatga caccgtatga gattcgacta ttctctcctg
     7261 agggcgtgtt gctatcagcg atatacaggg caaacgactt catgactccg ccacgagtcg
     7321 agtatcttcc gggcggcgga gtcacacttc acgcctatcc aatgtccgtg tcaatcatcg
     7381 tgctcggtga cgggcggttt gtaaacgcgg tgatacgacc agtcagtgct gaagaaagcg
     7441 aaacaatact cgacgtctat gaaggtgcgg gggtgctgat agggacaacc cgattagcgg
     7501 gcgcgcttgg cctgaagtgc cgtgacgagc gggggagatt gtacgcgtct gagcgtggcg
     7561 actttccgaa ggtggtgaag tacaaggtcc actttgaggg cggcaggaag tagttgttga
     7621 cgttaaggcg ttgctacgcg agccagaaag gcggcgaaag aatgaggaca ttgcatgtac
     7681 tagccgacca aagacctgtg cgtaaaacca gtgagtagtg atcactctgc cgtgggtcat
     7741 accgtccgag aacccacttt gttcgacccc acctcgactt tgcgctattg acgtacgcga
     7801 ccggcgcgca gcacctccgg cggtcttcgc cgaaatgtgc taccctaggc gtcgtcggcg
     7861 acggcgggcc ccatgctgag atcgttcaag cgagacttga gttccctgga gccgctgttc
     7921 gaattcgcgg gcgacttcgc ccggacgcac cggctggacg acgcgatcgt cttcgcgatg
     7981 aacctcgcgg tcgaggagct attcaccaac atggtcaagt atggcggggg agacgacatg
     8041 gtctccgtcg gcctggatgt gcgcggcgac gatctagtga tcgagctcgt gcacgcgggt
     8101 gcgaaccctt tcgaccccgg tgcggccgcg cccgtcgacg tcacccgaac gctggacgag
     8161 cgacggccgg gtggggtggg gctccatctc gtcaagagcg tgatggacga ctttcggtac
     8221 gagcacaaga acggcgacgc gcgcattacg gtcaccaagc acctcggccc gcgccctgaa
     8281 ggagcgtgaa tgttcgacat tcgtagggga accaacggcg tgattcttct cacggggcga
     8341 ttcgacgccg cccacgtgga gcgtgtgcgc gacgagctca actccgtcac gcaatcgtgc
     8401 acggtggatt tcaccgatct cgattacatc tccagcgcgg gaatgggggt gttgctgggg
     8461 acgcagaagc gcctggcagg ttcggggcac tcgctcaagc tcgtcaattt gaaccggcac
     8521 ataagagaga tcttccgcat cgccggtttc gacaacgtct tcttcataga atgaacgaag
     8581 atcgcaccaa cttttcgctc ggggtgggtg tacgtctccc atgagagatc acgccgatgc
     8641 cgagcttgtt cgggcgtgcc tctccgggaa ccagaaggca ttcgaggtcc tggtccggaa
     8701 gtacgagaag ccgatctaca acctcgcgct tcgcatgctg aaggaccggg acgacgcggc
     8761 cgacgtggcc cagacgacgt tcgtcaaggc gtaccagaag ctcgattcgt tcgacgaggc
     8821 gcacgagttc ttcagttgga tctaccgcat cgccatcaac gaatcgatca acttccagaa
     8881 gcgcaccaag cgtttggacg aatacgaaag cggagtgacc gcggccctgt cggcgacgca
     8941 ggaacaggcg cacggcgccg aagtcctccg ggacgagatg atcgacgcga tcgagaggct
     9001 gaccttggac tatcaaatgg tgatcgtact ccggcacttc cacgacttct cgtacgaaga
     9061 gatggcggac gtgctttcga tccccgagaa gacggtcaag tcgcgcctct tcaccgcgcg
     9121 ccagcagctc aaagacatcc tatccgcaaa ggggatcacg cgatgatcga cccgaaacac
     9181 atcgagctca tgaatcgcgt cctggacggc gccgccaccg aaccggagcg cgccgacctg
     9241 gatcgaatcc tcacgtcgtc gccggaggcg cacgcccact acgaccactt gcgccggctg
     9301 gtgagccggc tcgactcggt cccgatggtc gacccgcccg cggaactcta cccgcgcgtg
     9361 gtgtcggccg tcgacgcgtc ggggatccgg cacgcgcgct cgcacgagga gcacggcttt
     9421 tgggcctggc tccgacacgc gttcgcgccg ccggggcttc gctacgcctc gaccttcggg
     9481 ttggggctgg ccgcgggagc cttcgtcctg gccctggtcc gaccgggaac gccggtcgat
     9541 cccagccagg tctcgggcac cctcgcgaac ccgggcgcga cggggtcaat gcccgtcgcg
     9601 gtcccggagg ccggggtgtg gggttctgtc gccgtcgtcg accgcggccc ggtgacgcag
     9661 gtccatttgc aggtcgacgg ccaggggggc actgaatggg tattcgacgt tcccaatcgt
     9721 gccgaaccta gccagtccgt tgtcttacgt gtggttaagt ccggagagac ggtgttcgaa
     9781 ggagccgtcc gtccggtcga gccgtgaact tttcaatcag cgcagcccgt cggatcgaag
     9841 aaatcgcgcc tcgcaccaac tcccgatacc gacgtttgta gtaccgtcag aaccaggcga
     9901 gataccaggt cccaccgttc aaggaggaag cgtcatgtca aggaagtcta cgaccctgtt
     9961 gatcacgacg agcgtcgtca tcattgccgc ggtcgcgttt ttcacgtcca ataggaacaa
    10021 ccccaccagc gaccaggtcg ccggtgccat cggaacggtc gacaagtacc ggtccgagca
    10081 gatcacgccc agcgacgtcg acatccaggg ccagagcccg accagcgacg acgtgttcgc
    10141 gacgtggctg aacgacaccg cgacgttgga ggagctcgcg ggcttcatgg gccgcctgtc
    10201 gccgcaggag cgcttgaagt tcttcgatcg cgcgaccccc gaaatggccg cgatgatcgt
    10261 cgagcgcgcc acggttaccg agaaggcgag cctcttcaat cgtgcgactc cggaagagcg
    10321 cgcggccatg gtagcgcgca tgacgccgga cgagagaagg aagtacttgg gtaaggcgaa
    10381 cgtcgccgac ttggccgcgt tcgagaagat gagcgtctcc gagcgcgcga ccgccctcga
    10441 ccggttgatc cccgccgaga aggctgcgat cttcgagcgc gcgattccct ccgagaaggc
    10501 cgtggcgctg agccgctgcg acgtgaccga gcgcgcgacg attctcgacc gcgccacggt
    10561 tgccgagcgc gcgcaggtgc tgggtcgcgc caccgtcgcg gagcgcgcga gcctgtatga
    10621 gcgctgcacg cccgccgagc gcgccgccgt cgtcggtcgc atgtcgatcg aaggcaaggc
    10681 tgcgatgctg gaccgcgccg cggtggccga gaagtccgcg atcttcgagc gcgccacgcc
    10741 gtccgagaag gtcaccatgt acgacaacct gacggtgggc gagaaggcga ccgtgctgga
    10801 gaagaccaac atcaccatcg agagcttcgc caagatgcct ctcgaggagc gtgcgtcggt
    10861 gctatcgcgt gcgacgccgg aaggccgcgc ccagctcctg gaccgcgcca cggtgcaggc
    10921 caaggctgcg atcttcgagc gtgcgcccga gaacgagcgc gcgcagatct tcgagcgtgc
    10981 gatcgtcgcc gagcgcggca ccatcaccaa cgagaaggcg acgccgacga tggaaaaggc
    11041 ctccgcgacg aactagtcca cagtcctcgc aaggacgcct tcgacaacga tgggacgcca
    11101 cgggcagacc gtggcgtccc gcgtttttat ggtgtacact cccgcccgac ggcctgaccg
    11161 aacccagtcc gcatgcagat ccaagccccc ggcaatctcc gcggtttcgt cgccgcgccc
    11221 cttttcgcct tcgcgatcgt cctagcgctc gtcccgcgcg gcgcgcacgc gcaactcgac
    11281 ttccagagcc tcgagacctc caccatgcgc gtggtctatt acgaccccga gcacgcctac
    11341 atcattccgc acctcacgcg ctgtttcgag aactcgatgg cgttctacta cgacttcgtg
    11401 ggttacacgc cctacgagca cgtgaccatt ctcctgcagg acttcgacga ctacggctac
    11461 gccggcacgt cgacgattcc caacaactac ctgacactcg gcatcgagcc cttcgagtac
    11521 gtctacgaaa cgtgccccac caacgagcgt ttcaactggg tgatgagcca cgagctgtgg
    11581 cacgtggtgg cttccgagaa ggcgtcgcac accgaccgcg tatttcgcgg gctgttccag
    11641 ggcaaggtga tggcggacgc gaacaacccc gagtcgatga tctactcgta cttgtgctcg
    11701 ccgcgccgct tcgcgccccg ctggtaccac gaaggctccg cggtgttcat cgaaacctgg
    11761 ctcgcggggg ggattgggcg cgcgctgggc ggctacgacg agatggcctt tcgcgccatg
    11821 gtcgagagcg gggcctactt ctacgacttg gtgggcctgg agtcggaagg aaccacgagc
    11881 gacttccaaa tcggacagaa cagctatctc tacggcacgc gcttcatgtc gtacatcgcg
    11941 ctccagtacg ggcccgacaa agtggtcgag tgggtgaatc gtcgcgacga ctccaaggcg
    12001 tactacgctt ccgagttcaa gcacctcttc ggccagaaga tcgacgacga atggaagaag
    12061 tggatcgcct tcgagcacga gttccagaag cagaacctgg cgcgcatccg cgagtacccg
    12121 gtgacccagg agcgcgtggc gtgcgagaag gcgctggggt cggtttcgcg cgggtacgtg
    12181 gacaagcaga cgggcaagct cctggtcgct gtcaattacc cggcggagtt cgcgcacgtg
    12241 gcgtccgtcg accttgccac cggccgactc gagaagatcc acgacgtgca aacgcccgcg
    12301 ctctattacg tcacgtcgct cgcgttcgac gaggcgggcc gaacgctctt ctataccacc
    12361 aacaacggca agcactggcg cgacctcaac gcgatcgacc tgcgcacgcg cgcgagccgc
    12421 cgcctggtta ccaattgccg catcggcgac ctcgcgtact gccgggccga ttccacgctg
    12481 tggggggttc agcaccacaa cggcttctcg accatcgtgc gcatctcgcc cccctacgac
    12541 gactggaata agatcctgcc cgtcatggag ttcaagtacg gcaccgacgt tttcgacctc
    12601 gacgtctcgc cggacgggac cgtgctgacg gcgtcgatgc tcgagatcaa cggcgcggtc
    12661 aagctggtgc gcttcgacgt gcctgatctc atgaagggcc aggccggcta cagcgtgctg
    12721 cacgagttcg ccaacaccgc gcccgccaat ttcgtcttct ccgaagacgg cgcacacctc
    12781 tacgggacgt cgtaccagac gggcgtgtcg aacgtatttc gttggagttt cgcggaacag
    12841 aagatggagt gcgtcaccaa cgccgagatc ggctacttcc gccccattcc gatgggcgac
    12901 tcgctggtgg tgttctccta cacgagcgac ggcttccggc cggcggtgat cgccgatacc
    12961 acgctccagg acgtcaacgc gatccgctac ctcgggcagg cggtcgtgga cgagtggccc
    13021 gtcgtcaagg attggaagct gggctcgccg cgcgaggtga atctggattc ggtgacgacg
    13081 taccagggcg cgtaccacaa gttccgcagc atgaccacgg cctcgctcta cccgatcgtg
    13141 gaggcgtaca aggcgtacac ctcgatcggt ttgcgctgga acttcatgga cccgctcggc
    13201 atgcacggcg gcgagctgaa agtgggcgtg acgccgacct ccagcctccc ggacgacgag
    13261 ctcgtgcacg cgggcttcac ctaccggcat tttccgtgga cgctcaaggc ggcgtacaac
    13321 aaggccgact tctacgattt cttcggcccc accaaaacca gccgcaaggg ctacggggtg
    13381 gggctgatcc gctccgattt cttgatcgcc gacaagccgc gcttcctcga gtacaccctc
    13441 gacgtcgcgt actacggcgg gctcgagcgc ttgccctaca accagaacgt gctcgcgacc
    13501 ttcgacagct acgcgtcggc cagcgcgagc ctcgactaca agcgcacgca gagctcgatt
    13561 ggggcggccg actccgagaa gggagtccgc tggtcgctgg gagtggaggg gatcctcgtc
    13621 aaccaggact tctataccaa ggggatcgcg gagctcgact tcggattcct gacaccgatc
    13681 gaccactcgt cgatctggct gcggggcgcg ggcgggtatt cgcgcggcga ccgcgatgac
    13741 tccttcgcga gcttctactt cggcggcttc ggcaataact acgtcgacca ccaagaagtg
    13801 aagcgctacc gcgactacga tcgcttcccg ggggtcgaga tcgacgagat cgcgggcacc
    13861 aacttcgcca gggcgacggt cgagtggacg ctcccacccg tgcgcttccg ccgcatcggc
    13921 ttccagcaac tctattgcac gtatgcgcgc accgcggtgt tcgcgagcgg tattgcgacc
    13981 aacctggacg atgacgcgtt tcgccgcgag gtcggcaacg tcgggggaca ggtcgacttt
    14041 cggttggtga ttttctcggg actcgagtcc acgctatcct tcggctacgc cgcggccaag
    14101 gaagaag
//
LOCUS       DASPGK010000024         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1646326, whole genome shotgun sequence.
ACCESSION   DASPGK010000024 DASPGK010000000
VERSION     DASPGK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646326"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..701)
                     /locus_tag="VEC56_00630"
     CDS             complement(72..701)
                     /locus_tag="VEC56_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12684.1"
                     /translation="MRYQCTLFLALALAVAACEDDDPAAPIANSASQAYEIKFEQGAA
                     NGWFGGDSRPTFSSRTVGNGCSVLIGASIVLESFSFHFERAFYVAPNTGDTGHAVTLM
                     LNVRDATGVILKTVQLDLPSSYSGGWATWTGIDMNVDAGTTLIFTTYLVGAYDSNQVT
                     SGANGDVAAGYAGGVRYTKESTSDAAMEDWADWNEHPWDTWFWLQGTTR"
     gene            complement(766..2235)
                     /locus_tag="VEC56_00635"
     CDS             complement(766..2235)
                     /locus_tag="VEC56_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12685.1"
                     /translation="MSKNRSHVVVGSIVLAITGILAFMGGVLPVSAQTPKPKWTATDW
                     KEIDDPYKGGQRALAIQRLEEKLATKGNRYSDEILDGILPMLKDYFVEFEDAKRFQAI
                     IERTLRQDKQGKVVDYFQGTSLYATANKAREEWSKPREAEAKLTKSTLAVGDTTTYEI
                     TVLNKSGDRLSTEGMQVRATPAAKASVNKSTRQITALAPGRVNVTVVTAEGRQVGGMK
                     VIEVVEAPPVRVEIVPQASELIVGESTTFIVNLFDKENKPMTGGPAVNVDLFPTGLAT
                     LDKAKQEISAVNAGTVTLRALDKDDNVLGETAIRIKNATVTIDPILQTLKVGESASFV
                     VRTTRSFESQNLHLVMEPPGIASYTETSSQLEKQVQVTATKVGTARLLVKNSNGATVA
                     EAAVEVTTGPTAAAAADSGPSAIPMVVGAGVTAGLFVMAAVEEENNYLYAGIATAAIT
                     GYFAYKYFTGKKKQQALNAQQDGLRLSAGVSHVAITYNF"
     gene            complement(2269..2940)
                     /locus_tag="VEC56_00640"
     CDS             complement(2269..2940)
                     /locus_tag="VEC56_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12686.1"
                     /translation="MRLAGLLLTQALVMLVAGTVLTLVYPSLPATAGGPATLGVVAFG
                     LVGIVVGSFAIKSSVKRVAGLLGLVVIGVLNAAYLAWLYRQVIGSRGDEDFVSVVYLP
                     YLWAAVTLLGAIPLASARVKERRRLKSEFRPTEAGALAFIEANGLFATEAELRKLAAR
                     PKGLKCGICRNPYSGDEHLGSGWKERWVRCPRSPEHVFHARDFEYAEWRCPIDKSLLY
                     EPKGP"
     gene            complement(2943..>4199)
                     /locus_tag="VEC56_00645"
     CDS             complement(2943..>4199)
                     /locus_tag="VEC56_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXV12687.1"
                     /translation="KILPVEPLQQAVAVVGGAGALGNEVLKNLALLGVGHVVVCDFDR
                     VEIHNLTRAILFRQGDAGALKAEVAAVRMREINPGVNAIPFADNIAELGGGFFRRAGL
                     IFSTFDAFFPRYAVNEACLRYGKTWVDAGMSALEHTRGGVTVYDGADRASFCYACGTS
                     PEMVSRRLAAMRANVGCTAYENVTGEMGGVPTTPMMASVIGGVQTAAGLDIFFHRRGY
                     TPACEWPFGSWDLDISNLTHRRVRRQRMPDCYHHQVVESVTTKVVEIPEWDSQRTTYR
                     EVLDRAQEEFGTKRIAVDLPEDFYTVGTCSKCKEPWDLFRLKSTYMTRGKHMRCPVCG
                     EGEFAIDGAGMYAEIDYDWEYLDRPMAKAGARPLDVLKVVKYDESGAPDATRYWEVSG
                     DAKRLGLPESARATSVGVVPSIGAES"
BASE COUNT          726 a         1440 c         1223 g          810 t
ORIGIN      
        1 cgccgacgac ggcgcccaaa ccgctcgcca ctttctgcgc aacctctttc gacagctctt
       61 ctaacctgct cctaacgagt cgtcccctgc agccagaacc aagtatccca ggggtgttcg
      121 ttccaatccg cccagtcttc catcgcggcg tcgctggtgc tctccttggt gtatcgaacg
      181 ccccccgcat aaccggcagc gacgtcgcca ttcgcaccgg aggtaacttg attgctatcg
      241 taggctccga cgagataggt agtgaagatg agtgtggtgc ccgcatcgac gttcatgtcg
      301 atacccgtcc acgtcgccca tcccccactg tagctgctcg gcaggtcgag ctggacggtc
      361 ttgagaatga cgccggtcgc gtcgcgaacg ttgagcatca acgttacagc atgacccgta
      421 tcgccggtgt tgggcgcaac atagaaggcc ctttcgaaat ggaaagagaa gctctccaac
      481 acgatacttg ctccgatcaa cacactgcaa ccgttgccca cggttcgaga gctaaacgtt
      541 gggcggctgt cccctccaaa ccacccgttt gctgctcctt gctcgaactt gatctcatac
      601 gcttgcgacg cgctattggc gatgggtgcg gcggggtcgt cgtcttcgca cgcggccacc
      661 gccaacgcga gcgcgaggaa tagggtgcat tggtaccgca tggcgctacc tccaattgtg
      721 gaccggctct cgcaaggtat ccacgtttac tacattggag cgcccctaga agttgtatgt
      781 aatcgccacg tgggacacac cggcggatag gcgaagtccg tcttgctgcg cgttcaacgc
      841 ctgctgcttc ttcttgcccg tgaagtactt gtacgcgaag tacccggtta tggccgccgt
      901 cgcgatgccg gcgtacaggt agttgttctc ctcctcgacc gcggccatca cgaacaaccc
      961 ggccgtcacg ccggcgccca ccaccatggg tatggcggag ggcccggagt cggccgccgc
     1021 cgccgccgtg ggtcccgtcg ttacctcgac ggccgcttcg gccacggtcg cgccgttgct
     1081 gttttttacc agcaaccggg ccgtgcccac tttggtcgcc gtgacttgga cttgcttctc
     1141 gagctgcgac gacgtctccg tgtaggatgc gatgcccggc ggttccatca ccaggtgcag
     1201 gttctggctc tcgaacgagc gcgtggttct aaccacgaac gacgccgact ctccaacctt
     1261 gagggtctgc aagatgggat cgatcgtgac ggtggcgttc ttgatccgga tagccgtctc
     1321 tccgagcacg ttgtcgtcct tgtccaacgc ccgcaacgtg accgtacccg cgttcactgc
     1381 gctgatctcc tgcttcgcct tgtccagcgt cgccaagccg gtcgggaaaa ggtcgacgtt
     1441 gacggccggc cctcccgtca tgggcttgtt ctccttgtcg aacagattca cgatgaaagt
     1501 ggtggattcg cccacgatga gctcgctcgc ctgtggaacg atctcgacgc gcaccggcgg
     1561 ggcctcgact acctcgatca ccttcatccc gcccacctgc cggccctccg cggtgacaac
     1621 cgtcacgttc acgcgtcccg gcgcgagcgc cgtgatctgc cgtgtcgact tgttgacgct
     1681 ggccttcgcg gccggcgtcg cgcgcacttg cattccctcg gtgctgagcc tgtctccgct
     1741 cttgttgagc accgtgatct cataggtcgt ggtgtcgcca accgcgagcg tggacttcgt
     1801 caactttgcc tccgcttcgc gcggcttcga ccactcttcc cgcgccttgt tcgccgtcgc
     1861 gtagaggctg gtaccctgga aatagtcgac gaccttgcct tgcttgtcct gtcgaagcgt
     1921 cctctcgatg atcgcttgga accgcttcgc gtcctcgaac tccacgaagt agtccttcaa
     1981 catcggcagg attccgtcga ggatctcgtc cgaatagcga ttacccttcg tcgccaactt
     2041 ctcctccagc cgctgaatcg cgagcgcccg ctgtcccccc ttgtacgggt cgtcgatctc
     2101 cttccaatcg gtcgcggtcc acttcggctt gggcgtttgt gcactcaccg gcaatacgcc
     2161 acccatgaag gcaagtatgc cggtgatcgc aagaacaatg cttccaacga cgacatgaga
     2221 gcggttctta ctcattgatt tcgcctttct ttggggagcg cgaacacgtc acggaccctt
     2281 cggttcgtaa agtaggctct tatcgatcgg acaacgccat tcggcatatt cgaagtctcg
     2341 ggcgtggaac acgtgctcgg gcgaccgcgg acaacggacc caacgttcct tccagccgct
     2401 gccaagatgc tcgtcgccgg agtacggatt ccggcagatc ccgcacttga gacccttggg
     2461 gcgggccgcc aacttgcgca actcggcctc ggtcgcgaac agcccgttgg cctcgatgaa
     2521 cgcgagcgcc ccggcctcgg tgggccggaa ttccgacttc agtcgacggc gctctttgac
     2581 ccgcgccgac gcgagcggta tcgcgcccag caatgtcacc gccgcccaca agtagggcaa
     2641 gtaaacgacg gacacgaagt cctcgtcccc gcgcgaaccg atgacctgtc ggtacagcca
     2701 cgccaggtag gcggcattga gcactccgat gacaaccaag cccaagaggc ccgcgacacg
     2761 cttcacggac gatttgatcg cgaacgaccc gaccacaatg ccgacgaggc cgaacgcgac
     2821 cacgccgagg gtcgccggcc cgcccgccgt cgccggcaag gacggataca ccagtgtgag
     2881 cacggtcccg gcgaccagca tgaccagcgc ttgggtcagc agcagtcccg cgaggcgcac
     2941 cgtcaactct ccgcaccgat cgagggaacg actcccacgg acgtcgcccg cgcactctcg
     3001 ggcaacccga gacgcttcgc gtcgccgctg acctcccagt agcgcgtcgc gtcgggcgca
     3061 ccggattcgt cgtacttgac caccttcaga acgtcgagtg ggcgcgcgcc cgccttcgcc
     3121 atcgggcgat ccaggtactc ccagtcgtaa tcgatctcgg cgtacatgcc cgctccgtcg
     3181 atcgcgaact cgccctcgcc gcagaccggg caccgcatgt gcttgccgcg cgtcatgtac
     3241 gtcgacttga gccggaacag gtcccacggc tccttgcatt tcgagcacgt gccaacggta
     3301 taaaaatcct cgggaaggtc gacggcgatt cgcttcgtgc cgaactcctc ttgcgcgcgg
     3361 tccagaacct cgcggtaggt cgtccgctgc gagtcccact ccggaatctc gaccaccttc
     3421 gtcgtcacgg actcgaccac ttggtggtgg tagcaatccg gcatgcgttg ccggcgtacg
     3481 cgacgatgcg tgaggttgct gatgtcgagg tcccagctgc cgaacggcca ctcgcacgcg
     3541 ggcgtgtagc cgcgacggtg aaagaaaatg tcgagcccgg cggccgtctg cacgccgccg
     3601 atcaccgacg ccatcatcgg cgtggtgggt accccgccca tctcgccggt cacgttctcg
     3661 taggccgtac agccgacgtt cgcgcgcatc gccgccaggc ggcggctcac catttcggga
     3721 gaggtgccgc aggcgtagca gaaactggct cggtctgcgc cgtcgtacac cgtgaccccg
     3781 ccgcgcgtgt gctccagcgc cgacatgccg gcgtcgaccc aggtcttacc gtagcgaagg
     3841 cacgcctcgt tcacggcgta gcgcgggaag aaggcgtcga acgtggagaa gatcaacccg
     3901 gcgcggcgaa agaacccgcc gcccaattcc gcgatgttat cggcgaaggg gatggcgttc
     3961 actcccggat tgatctcgcg catgcgtacc gcggcgacct ccgccttgag tgcgcccgcg
     4021 tcgccctggc ggaacaggat cgcgcgcgtg aggttgtgga tctccacccg gtcgaaatcg
     4081 cacaccacca cgtgcccgac gccgaggagc gcgagatttt tcaggacctc attccccaac
     4141 gcgccggctc cacccacgac ggccaccgcc tgttgcagag gttcgaccgg gaggatctt
//
LOCUS       DASPGK010000025         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_943456, whole genome shotgun sequence.
ACCESSION   DASPGK010000025 DASPGK010000000
VERSION     DASPGK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943456"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VEC56_00650"
     CDS             complement(<1..813)
                     /locus_tag="VEC56_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12688.1"
                     /translation="MHLRRPAVFLLLLALFVAALVTRSATRDRLESPYFKGTSAMVYA
                     HALAASEGTPLDTVSSKANRPEGYTPARYRADGFEQAWAASFRAARFVSEADGRDFAR
                     RVTMFLAALCVFTMYALARRVWDCQAAGFVAAFLVAFLAPFVLATNGRVFTHTVLVPF
                     VDSAHALAALLALRATTRRGAIVFGALTTAASFALASAWEPGMVVLATWSGGIALSGL
                     GEPRARAAVLWGHALAAVLASVLSPHLLATRAIGSWETAIVVASALLPYSARV"
     gene            complement(835..1599)
                     /locus_tag="VEC56_00655"
     CDS             complement(835..1599)
                     /locus_tag="VEC56_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXV12689.1"
                     /translation="MPKTVRRDDLVAYLDRYLRVAEIPDSSPNGLQVQGTDRVARLAF
                     AVDVSVKTVRAAAKARANFLVVHHGLWWGRHEPIVGNLHARVKALFESEMSLYAAHLP
                     LDCHGDVGNNVGLARLLSLRVVEPFGEYKGVKIGVVASSTPSVRLSTLVEKVERKLTT
                     QARVLGFGPATVRRVAIVSGGGGAFVEAAVRARCDTLLTGESSHAAYHAAEEAGINVI
                     FAGHYATETVGLESLQRHLRTRFGLAAEFVSAPTGY"
     gene            complement(1615..>4490)
                     /locus_tag="VEC56_00660"
     CDS             complement(1615..>4490)
                     /locus_tag="VEC56_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV12690.1"
                     /translation="FLDAIKRDVVDSGAIAALPYGEQLAIMVRIGDDFLARGRQPEAE
                     SLLVRARQLRTTGDEVRAHAPLASEAMRLLADAWAGRGQLRQALELLEDTKEDIAPYL
                     SLAEQARLLNEIGWLQYRLGDYSSCVDSCKFTVNTLNPNEHPLVVAQALNLLGVIAFN
                     TSRYDEAVSYYEQSAFLRERENDLNALAGSYNNLALAYQSKGEYDKALGYLHKSIDIK
                     RKQNNEAGLAGGFLNLAFLYLEVHNFEEAERRCRESLRISSELGLAQLIAENYNTLGD
                     ILLTRGQLDEAEAYYQRSLEHASRLSTVNEEMGAQKRLAKLYIRKNRHGDARAAIEEA
                     SRLARQVGSKYEQAQTEEIFGELKAAQKSHAESVDHFEKAAALYATLSKHRMAAACLA
                     KAGIAHAGSGNPLDAKRYLDRAQDLIKAQIGHEIPEEIVTLQQTLRESPRPAGSTGTA
                     SQKLMYAFYELSSLSDYADDKTAFFRRIINVFRDLTGSNWCALALRSEDEGFAMVSAT
                     GDQEPIEDAALKALMTHALQLGAVLYAESAEARDMVAHVAIQKASQDHLVVVPMKSMG
                     VDLGCVLLLVPSTAVPRSKDDVDFISCIGRHVAGDLRLSLHFDERSHKVERELGAVAP
                     VSDANRFQGMIGRDESMRKVCRMIEKIKDMDTGILIIGESGTGKTELARTVHNTSPRR
                     RHVFQSIHCAEIPSSLLESELFGHERGAFTGAVQRKLGRCEIADGGTVFLDDVNVMPL
                     DTQARLLHYLETKSFTRLGGTQKISTDVRLIAASNEDLEKLVKEGRFREDLFYRLKVI
                     QIELPPLRDRQDDLILIANEFLKKRCQAQNKPLKRLSTETIKLFQKYRWPGNVREFQN
                     VLEQIVLLCDDVIVEPSSLPEDFLRRAAGGGKSRWQSLEQLVQQMVEAGGYSEANPLM
                     PQIEVMLAEKMSTHTENKTKAANLLGITKPTLYSRLRGYDRLK"
BASE COUNT          707 a         1498 c         1529 g          756 t
ORIGIN      
        1 gacccgcgcc gagtaaggca agagcgcgct cgcgaccacg atcgccgtct cccacgagcc
       61 aatggctcgc gtcgcgagca aatgcgggga gagcacggac gccagcaccg cggccagggc
      121 gtgaccccac agcaccgccg cgcgcgcgcg cggctcgccg agacccgaca gcgcgatccc
      181 gcccgaccag gtcgccagca cgaccatgcc gggctcccac gccgacgcga gcgcgaagga
      241 cgccgccgtc gtcagcgcgc cgaacacgat cgcgccgcgc cgcgtggtcg cgcgcagcgc
      301 caacagcgcc gccagcgcgt gcgcgctgtc gacgaaggga accagcacgg tgtgcgtgaa
      361 gacccgtccg ttggtcgcca gcacgaaggg cgcgaggaac gccaccagga acgcggccac
      421 gaatcccgcc gcctggcagt cccacacccg acgcgcgaga gcgtacatcg tgaacacgca
      481 caaggcggcg aggaacatgg tgacgcgacg cgcgaagtcg cgcccgtctg cttcgctcac
      541 gaagcgggcc gcgcgaaacg aagccgccca tgcctgctcg aagccgtcgg cgcggtagcg
      601 cgcgggggtg tagccctcgg ggcggttcgc cttggacgag acggtgtcga gcggggtgcc
      661 ttccgaggcg gccagcgcgt gcgcgtacac catcgcggag gtgcccttga agtagggaga
      721 ttcgagccgg tcgcgggtgg cggaacgggt gacgagtgcg gccacgaaga gcgccaggag
      781 aagcaagaag acggccggtc gcctgaggtg catgtgcgat accgcgggcg gcgatcagta
      841 tcccgtgggt gcggaaacga actccgcggc caggccgaaa cgggtacgca ggtggcgctg
      901 gagcgactcc aggccgacgg tctcggtcgc atagtgtccg gcgaagatca cgttgatgcc
      961 cgcctcctcg gccgcgtggt aggccgcgtg cgacgattcg ccggtcagaa gcgtgtcgca
     1021 gcgcgcccgc accgccgcct ccacgaaggc gccgccgccc ccgctgacga tggccacgcg
     1081 ccgcaccgtg gcggggccga agcccagcac gcgcgcttgg gtcgtgagtt tgcgctcgac
     1141 cttctcgacc agcgtgctca atcgcaccga cggcgtcgac gacgccacca cgccgatctt
     1201 cacgcccttg tactcgccga acggctccac cacgcgcagc gacaggagac gcgcgagtcc
     1261 cacgttgttg ccgacgtcgc cgtggcaatc gagcggcagg tgcgcggcgt aaagcgacat
     1321 ctcgctctcg aacaaggcct tcacgcgcgc gtgcaggttg ccgacgatgg gctcgtggcg
     1381 gccccaccac aggccgtggt ggacgacgag gaagttggcg cgggccttgg cggcggcgcg
     1441 gaccgtcttc accgacacgt cgaccgcgaa ggccagccgc gcgacgcgat cggtgccttg
     1501 cacttggagc ccgttgggcg acgaatccgg gatctccgcc acgcgcagat agcggtcgag
     1561 gtaggcgacc aggtcgtcgc ggcgcaccgt cttgggcatc gagtggtttc ccgtctactt
     1621 caagcggtcg tagccgcgca gccgcgaata gagcgtgggt ttggtgatac cgagcaagtt
     1681 ggcggccttg gtcttgttct cggtgtgcgt cgacatcttc tccgccagca tgacttcgat
     1741 ctgcggcatg agcggattcg cttccgaata gccccccgcc tcgaccattt gctgcaccaa
     1801 ttgctcgagc gactgccagc ggctcttgcc cccgcccgcg gcgcggcgca ggaagtcttc
     1861 gggcagcgac gacggctcga cgatgacatc gtcgcacaag agcacgatct gttcgagcac
     1921 gttctggaat tcgcgcacgt tgccgggcca gcgatacttc tggaacagct tgatggtctc
     1981 ggtcgacagc cgcttgagcg gcttgttctg cgcctggcag cgcttcttca agaactcgtt
     2041 cgcgatcagg atcaagtcgt cctggcggtc gcgcaagggc ggcagctcga tctggatcac
     2101 cttcaggcga tagaacaagt cctcgcggaa gcggccttcc ttcaccagct tctccaggtc
     2161 ctcgttgctg gccgcgatga ggcgcacgtc ggtcgagatc ttctgggtgc cgcccaggcg
     2221 ggtgaaactc ttggtctcca agtagtggag cagccgcgcc tgcgtgtcga gcggcatcac
     2281 gtttacgtcg tccaggaaca cggtgccgcc gtcggcgatc tcgcagcggc cgagcttgcg
     2341 ctgcaccgca cccgtgaagg cgccgcgctc gtgcccgaac aactcgctct cgagcaggct
     2401 gctgggaatc tcggcgcagt ggatcgactg gaacacgtga cgccggcgcg ggctggtgtt
     2461 gtgcaccgtg cgcgcgagct cggtttttcc ggtgccgctc tcgccgatga tgaggatacc
     2521 ggtgtccatg tccttgatct tctcgatcat gcggcacact ttgcgcatcg actcgtcgcg
     2581 cccgatcatg ccctggaatc ggttcgcgtc cgagaccggt gccaccgctc ccagttcgcg
     2641 ctccaccttg tgcgagcgct cgtcgaagtg gagactcagt cgcagatcgc cggcgacgtg
     2701 gcggccgatg caggagatga agtcgacgtc gtccttggag cgcggcacgg cggtcgacgg
     2761 cacgaggagc agcacgcagc cgaggtcgac gcccatcgac ttcatcggca ctaccaccaa
     2821 gtggtcctgc gaggccttct ggatggcgac gtgcgcgacc atgtcgcgcg cctccgcgga
     2881 ctccgcgtac agcaccgcgc cgagctgcag cgcgtgcgtc attagagcct tgagcgccgc
     2941 gtcctcgatc ggctcctggt cgccggtcgc cgacaccatc gcgaagccct cgtcctcgga
     3001 gcgaagtgcg agcgcgcacc agttcgagcc cgtgaggtcg cgaaacacgt tgatgatgcg
     3061 gcggaagaac gcggtcttat cgtccgcgta gtcggataag gaagagagct cgtaaaaagc
     3121 gtacatcagc ttctgcgacg ccgtgcccgt cgaccccgcg ggccgcggac tctcgcggag
     3181 cgtctgctgc aaggtgacga tctcttccgg gatctcgtgg ccgatctgcg ccttgatgag
     3241 gtcctgtgcg cggtccaggt agcgcttggc gtcgagcggg ttgcccgagc cggcgtgcgc
     3301 gatcccggct ttggccaggc acgcggccgc catgcggtgc ttggacagtg tcgcgtacag
     3361 cgccgccgcc ttctcgaaat gatcgaccga ctccgcgtga ctcttctgcg ccgccttgag
     3421 ctcgccgaag atctcctcgg tctgcgcctg ctcgtacttc gatcccacct ggcgcgcgag
     3481 ccgcgaggcc tcctcgatgg ccgcgcgcgc gtcgccgtgg cggttcttgc ggatgtagag
     3541 cttggcgaga cgcttctggg cgcccatctc ctcgttgacg gtcgacaatc ggctcgcgtg
     3601 ctcgagcgag cgctggtagt acgcctccgc ctcgtcgagc tggccgcgcg tcaggagaat
     3661 gtcgcccagc gtgttgtagt tctcggcgat caactgggcc aggcccagct ccgagctgat
     3721 gcggaggctc tcgcggcagc gacgctcggc ttcctcgaag ttgtgcactt ccagatagag
     3781 gaacgcgagg ttcaagaagc cgcccgcgag cccggcttcg ttgttctgct tgcgcttgat
     3841 gtcgatgctc ttgtgcaggt accccagcgc cttgtcgtac tcgcccttgg actggtaggc
     3901 gagggccagg ttgttgtacg acccggccag cgcgttcagg tcgttctcgc gctcgcgcag
     3961 aaacgcgctt tgctcgtagt acgacaccgc ttcgtcgtag cggctggtgt tgaacgcgat
     4021 gacgccgagt aggttcagtg cttgcgccac caccagcggg tgttcgttgg ggttgagcgt
     4081 gttgaccgtg aacttgcacg aatccacgca gctcgaatag tcgcccaagc ggtactgcag
     4141 ccagccgatc tcgttcaaca ggcgcgcctg ctcggccagg ctcaggtagg gcgcgatgtc
     4201 ttccttggtg tcttcgagca gctcgagcgc ctggcgcagc tggccgcgtc ccgcccacgc
     4261 atcggcgagc aggcgcatcg cttcgctggc cagtggcgcg tgcgcgcgta cctcgtcgcc
     4321 ggtggtgcga agctggcgcg cgcgcacgag cagcgattcg gcttcgggct ggcgcccgcg
     4381 cgcgaggaag tcgtctccga tgcggaccat gatcgcgagc tgctcgccgt aggggagcgc
     4441 cgcgatggcg cccgagtcga cgacgtcgcg cttgatggcg tctaggaagg
//
LOCUS       DASPGK010000026         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_826948, whole genome shotgun sequence.
ACCESSION   DASPGK010000026 DASPGK010000000
VERSION     DASPGK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826948"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1545
                     /locus_tag="VEC56_00665"
     CDS             118..1545
                     /locus_tag="VEC56_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV12691.1"
                     /translation="MVAWHDFRFFASDIYCQRVSITGASYWAANGVAVCSAAGAQQNP
                     VVVPDGAGGFILAWTDGRGGSTPDFSNVFAQRLSAAGASQWTPDGISVCSAPDNQGYP
                     SIAPDGANGTIFAWGDVRSASFEVYAQRIDNVYGYWGHPEPIVESVDDIPHDQGGKVA
                     VNWTASGRDLPNPRAIDFYSVWRAVDAIPSGAAAISSGELSALSADETGPVYLATPGR
                     YFERVGTQTAHGWPGYSFAASTRSDSVAASPGNEVFMVAAHIPYDTYVAFASNEVAGH
                     SVDNLAPLAPFLLTAQRVGADVQLTWNRAVAPDLRDYAVYRATASGVTPVPIHFLTSA
                     EDTLAVDANAPSSALYYIVTAYDVHANQSAPSNEANVGALTDAGNTPALAALTVLQNH
                     PNPFNATTEFEIGLPSAAKVRIEVFDVAGRRVSTLEVDEAKAGWNRIPFASRDNRGRP
                     LASGVYFYRVKAVGASITKKMVIAR"
     gene            1629..>2501
                     /locus_tag="VEC56_00670"
     CDS             1629..>2501
                     /locus_tag="VEC56_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12692.1"
                     /translation="MARQRCLFNVAILMVIALAGCESDSGLLAPRGSERETSASINSI
                     SELGQGAYEFRALDVPADMGSYTSAFGNNNLGTIVGNFAAPDGSAHGFVHRLGRFIDV
                     TVPGAGPSDRGSLADINDLGIAVGSFYDEDDIGHTYVRGPLGNIIVLPDREPDVRSCE
                     GYGINNRGTIVGNFVDAAGVRHGYIWRLGQYATFDAPGARSTRLNAVNDRGEIAGQYT
                     DMSSRVRGFVLRDGMVQPVEFPDAVSTRATGINNRGQITGFYNNDDGVWHGFVCSDGQ
                     YAAIDFPGSIDTAAF"
BASE COUNT          435 a          818 c          810 g          438 t
ORIGIN      
        1 gcccagcgtg cgacttcgaa cggctctatc caatggggtt ctgcgggcgc cgctgtgtgc
       61 gtcgaggctc aagaccagcg ctggccggcc attggtagcg acggaaccgg aggcgccgtg
      121 gtcgcctggc acgactttcg gttcttcgcc tccgacatct attgccagcg ggtgagtatc
      181 accggcgcgt cctattgggc cgcaaacggg gtggccgtat gtagcgccgc gggcgcgcaa
      241 cagaatcccg ttgtcgtccc cgatggggcc gggggtttca ttctcgcctg gaccgacggc
      301 cgtggtgggt ctactcccga tttctccaac gtgttcgcac agcgactcag cgctgccggc
      361 gcgtcgcaat ggacgcccga cgggatcagc gtgtgcagcg cgccggacaa tcagggttat
      421 ccctcgatcg ctccggacgg cgcgaacggc acgatcttcg cgtggggtga cgtgcgcagc
      481 gcgagcttcg aggtctacgc acagcgcatc gacaacgtat acggctactg gggccacccg
      541 gagccgatcg tcgagtcggt cgatgacatt ccgcacgatc agggcggaaa agtcgccgtc
      601 aattggacgg cgagcgggcg cgacctgccg aacccgcgcg ccatcgattt ctactcggtg
      661 tggcgagccg tcgacgcgat tccctccggc gcggccgcca tttcgtccgg cgaattaagc
      721 gcgctctccg ccgacgaaac cggaccagtc tacctcgcga cccccgggcg ttatttcgag
      781 cgcgtcggca cgcagaccgc gcacggatgg ccgggttact ccttcgccgc ttcgacgcgt
      841 tcagactcgg tggcggcctc gccggggaac gaggtattca tggtcgcagc gcacatcccc
      901 tacgacacct acgtcgcttt cgcctcgaac gaggtggctg ggcactcggt cgacaacctg
      961 gcaccgctcg caccgttcct gttgaccgcg cagcgggtcg gcgccgacgt tcagttgacc
     1021 tggaatcgtg ccgtcgcccc cgatcttcgg gactacgcgg tctaccgcgc gacggcgtcc
     1081 ggtgtgacgc cggtgccgat ccacttcctc acgagcgccg aggacacgct ggccgtggac
     1141 gccaacgccc cctcctcggc tctctactac atcgtcaccg cgtacgacgt gcacgcgaac
     1201 cagagtgcac cttccaatga agccaatgtg ggagcgctga cggacgcggg caacacgccg
     1261 gcgttggccg cactcacggt tttgcagaac caccccaatc cgttcaacgc gacgaccgag
     1321 ttcgagatcg ggctgccgtc ggccgcgaag gtaagaatcg aggtgttcga cgtcgcgggg
     1381 cggagagtga gcaccctcga agttgacgaa gcgaaagccg gttggaatcg aatcccattc
     1441 gcttcgcgcg acaaccgcgg tcggccgctc gcgagcggtg tctatttcta tcgagtcaag
     1501 gcggtcggcg cgtccatcac aaagaagatg gtgatcgcac ggtaaggtcg ctgcccggcc
     1561 taggcacgat caacacgact ctctggtagg cagtgtccaa tcgcaacgat ggtccaagga
     1621 ggaaaagcat ggctaggcag cggtgtctct tcaatgtggc gatcctgatg gttatcgcgc
     1681 tcgcgggctg cgaatcggat tccggcttgc tggcgccgcg cgggtccgag cgggaaacct
     1741 cggcctcgat caattcgatc agcgagctcg gccaaggcgc ctacgaattc cgcgcgctcg
     1801 atgtccccgc ggatatgggg tcctacacca gtgccttcgg caacaacaac ctcggcacga
     1861 tcgtggggaa cttcgcggca ccggacggga gtgcccatgg gttcgttcat cgtctgggac
     1921 ggttcatcga cgtcaccgtc ccgggcgcgg gcccttccga tcggggttcc ctcgccgaca
     1981 tcaacgactt gggaatagcg gtgggcagtt tctacgacga ggacgacatc gggcacacgt
     2041 acgtgcgcgg accgttggga aatatcatcg tgctgcccga tcgcgaaccc gatgtgcgct
     2101 cctgcgaggg ttacggcatc aacaaccgcg gcaccatcgt cggcaacttc gtcgacgccg
     2161 cgggggtgcg gcacgggtac atctggcgcc tcggacagta cgcgacgttc gacgcccccg
     2221 gcgccaggag cacgcgtctc aacgcggtca acgatcgcgg tgaaatcgcg gggcagtata
     2281 ccgacatgtc atcgcgcgtc cgcggcttcg tactgcgcga cggcatggtg cagccggtcg
     2341 agtttccgga tgcggtgagc acgcgggcca ccggcatcaa caatcgcggt cagatcacgg
     2401 gcttctacaa caacgacgac ggcgtgtggc acgggttcgt ctgcagcgac ggccagtacg
     2461 cggccatcga cttcccgggc tccatcgaca cggccgcatt c
//
LOCUS       DASPGK010000027         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_7180, whole genome shotgun sequence.
ACCESSION   DASPGK010000027 DASPGK010000000
VERSION     DASPGK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7180"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /gene="purL"
                     /locus_tag="VEC56_00675"
     CDS             complement(<1..1949)
                     /gene="purL"
                     /locus_tag="VEC56_00675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXV12693.1"
                     /translation="MIDPKDLVPVEPKDADGAKDFLKKNGVSLTTGEYARVCELLGRR
                     PTIVELHIFNVMWSEHCSYKSSRWVLKEYLPTQAPHVVLGPGEDAGIIRFCEVGGKSY
                     CLVMAHESHNHPSQVLPVEGAATGIGGIVRDVYCMGADVIGVMDPLRFGDPDGPNAAH
                     VRDIALGVVDGIAQYGNALGVPTYGGEVFFDASFDDNCLVNVVAFGIVEESGIIRSRV
                     PEEAKHVPYDLILVGKPTDASGFGGAAFASERLDGSAATEKQGAVQVPDPFVKRVLAE
                     AIKDMLEFARDHKVPIGFKDLGAGGISCAFSEMPEASGFGAKIDLDKVNKAFANLLPE
                     VIACSETQERFAIAVPSRVSRDVVRIFNERFEMPHLYTGAGAVIIGRVTTDGRFQITH
                     GGRTVCDAAMDVITTGIAYKREQKKRQRKLAIPPSAPKANVRESLLALLGSVNISDKA
                     AIFQHYDCEVQGHAVLRPGEADAAVEMFVPGANVALVASVGSHSRIGLIDPYAGGAWS
                     VFEATRNAACVGALPLCITDCLNFGDPEDPGVFHEFVESVRGIGDACRALTLFGDNSP
                     LPVLSGNVSLYNQSEKGSAIAPTPIVACAARLDDASRARGFGLKQVGSRLLLLGKLHE
                     DLGGSEYERWFGNHGRERAPQPDFA"
     gene            complement(2059..>2240)
                     /locus_tag="VEC56_00680"
     CDS             complement(2059..>2240)
                     /locus_tag="VEC56_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV12694.1"
                     /translation="VTLASGEKALNPMRVVPNGEGSEVLFTLFQLSEMSDLQFVEDVA
                     MVERDLRSLKEVLEG"
BASE COUNT          384 a          742 c          724 g          390 t
ORIGIN      
        1 aacgcgaaat cgggctgggg agctcgttca cgaccgtgat tgccgaacca ccgctcgtac
       61 tccgacccgc ctagatcctc gtggagcttc cccagcagca acaaccgcga tccaacttgc
      121 ttcagcccga agccgcgcgc gcgcgaggcg tcgtccaggc gcgcggcgca ggcgacgatc
      181 ggcgtgggcg cgatggcgct ccccttttcg ctctggttgt agagcgagac gttgccggag
      241 aggaccggca acggcgagtt gtcgccgaag agggtgagcg cacggcatgc gtcaccgatg
      301 ccgcgcacgc tctcgacgaa ctcgtggaag acgcccgggt cttcggggtc accgaaattc
      361 aaacaatcgg tgatacacaa tggcagcgcc cccacgcacg cggcgttgcg cgtcgcttcg
      421 aacaccgacc acgcgcctcc cgcgtacggg tcgatcaggc cgatgcggga gtggctcccg
      481 accgacgcga ccagcgcgac gttcgcgccc ggcacgaaca tctccacggc cgcgtccgct
      541 tcaccggggc gcagcaccgc gtgaccttgc acttcgcagt cgtaatgttg aaagattgct
      601 gctttgtccg aaatatttac ggagcccaac agcgcgagca acgattcgcg cacgttcgcc
      661 ttcggcgcgc tagggggaat cgccagcttt cgctggcgtt tcttctgctc gcgcttatac
      721 gcaatgcccg tcgtgatcac gtccatggcg gcgtcgcaca ccgtgcggcc gccgtgggta
      781 atctgaaaac gcccgtcggt ggtcacgcgc ccgatgatga ccgcccccgc gcccgtgtac
      841 aggtgcggca tttcgaagcg ctcgttgaag atgcgcacca cgtcgcggga gacgcgcgag
      901 ggcaccgcga tcgcgaagcg ctcctgcgtc tccgagcacg cgatcacttc gggcaagaga
      961 ttcgcgaagg ccttgttgac cttgtcgagg tcgatcttgg cgccgaaacc gctcgcctcc
     1021 ggcatctccg agaacgcgca cgagatgccg cccgcgccca ggtccttgaa gccgatcggc
     1081 accttgtggt cgcgcgcgaa ctcgagcatg tccttgatcg cttccgcgag cacgcgcttg
     1141 acgaaggggt cgggtacttg cacggcaccc tgcttctccg tcgccgcgct tccatcgagg
     1201 cgctcggacg caaacgcggc cccgccgaat ccgctcgcgt cggtcggctt gcccaccagg
     1261 atcaaatcgt aggggacgtg cttcgcctct tcgggaacgc ggctgcgaat gatgccgctc
     1321 tcttccacga tgccgaaggc gaccacgttg accaggcagt tgtcgtcgaa ggacgcgtcg
     1381 aagaacacct cgccgccgta ggtgggaacc ccgagtgcgt tgccgtactg cgcgatcccg
     1441 tcgaccacgc ccaaggcgat gtcgcgcacg tgggccgcgt tgggcccgtc ggggtcgccg
     1501 aagcgcagcg ggtccatgac accgatcacg tccgcgccca tgcagtacac gtcgcgcacg
     1561 attccgccga tgccggtcgc ggcgccttcg acggggagga cctgcgaggg atggttgtgg
     1621 ctctcgtggg ccatgaccag gcagtagctc tttccgccga cctcgcagaa gcggataatg
     1681 ccggcgtctt cgccggggcc gagtacgacg tgcggggctt gggtgggaag gtattccttg
     1741 agcacccagc gcgaggactt ataggagcag tgctcgctcc acatcacgtt gaagatatgg
     1801 agctcgacga tggtgggcct gcggccgagc agctcgcaca cgcgcgcgta ctcgccggtc
     1861 gtgagcgaca cgccgttctt ctttaaaaag tctttcgccc cgtccgcgtc tttgggttcg
     1921 acggggacca ggtccttggg gtcgatcatt cgtgggatct cgtcggaaaa gaaatggacg
     1981 ccgcggccac gcggcgtctg acgggactat acgacacggg ggccggacgc gcaagacggg
     2041 gagaatgtgg cgtccggact agccttccag gacttctttg aggctccgca ggtcccgctc
     2101 gaccatggcg acgtcttcga cgaattgcag gtccgacatt tcggaaagct ggaagagcgt
     2161 gaacagcacc tcgctcccct cgccgttggg cacgacgcgc atcgggttca gcgctttctc
     2221 gcccgacgcg agcgtgaccc
//
LOCUS       DASPGK010000028        14370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_710115, whole genome shotgun sequence.
ACCESSION   DASPGK010000028 DASPGK010000000
VERSION     DASPGK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710115"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VEC56_00685"
     CDS             <1..1108
                     /locus_tag="VEC56_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV12695.1"
                     /translation="AELNADHLLPALADSCLGAEFETPLVELARRIAGTRATGAHPRD
                     AAPQRAIAASDRSPRRALDLTPAREGLSHRERYAKLALGDERVEVGYVATTRGCKHTC
                     RHCPLPPAYAGSFYALPIAPIMDDVARLVARGARHVTFADPDFLNGPTHALRVAREVV
                     RRFPGVTFDYTAKIAHLLRHDEVVDELHELGNLFVVSAVESFNDAVLAALQKGHTRAD
                     ALSVVRRFRARGLTLRPTFVPFTPWETRESYTEMFDIVEGEGLVGHIEPVQYAIRLLV
                     PERSLLLGAAEMRPHLGGFDTLTFSYAWHHPDPAMDDLQRTVARTAADLVGAGVDHDH
                     AFLRLREAAGVTRARGPARARAGRAPRLTEDWFC"
     gene            1220..1852
                     /locus_tag="VEC56_00690"
     CDS             1220..1852
                     /locus_tag="VEC56_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXV12696.1"
                     /translation="MDELTFNFELPEGRSVRITATPSMMHPEQCTFTVSEPVYPGRSA
                     YFGDRDQSRESVLVDRLFAIEGVADVLVNHDTVRVTLVGDANWEDKAPRVGAAIRDGL
                     AAGAPISQAVTESLLDPQETRRRVQQVLDTVINPAVASHGGVVRLLDVANNTVFLEFG
                     GGCQGCGMVSVTLKYGVERTIRDEVPEVGAIMDTTDHAAGRNPYYAPSSK"
     gene            1881..3128
                     /locus_tag="VEC56_00695"
     CDS             1881..3128
                     /locus_tag="VEC56_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV12697.1"
                     /translation="MKPRLLLLVPTSTYRAEAFIRAAQRLPIDLSIASESPNSLSHLH
                     PVDLPTFDFTSPADAAARAYALAKTHPVHAVVAVDDQATLAAAVISEALGLAHNPAHA
                     VRAALDKYRARERMRAVGIAQPDYRLVSFDGYVDAAQSLSYPAVVKPLAMAASRGVVR
                     VDNASQFVDAFARVASLVSSNASHLDESARSHLLVEAYVPGWEVAVEGLVVKGRARVF
                     AVFDKPDPLEGPYFPETMYVTPSRLGDATVDRIVRLTAEAVHAVGLTHGPFHVEMRGD
                     GDRLLPIEVHARSIGGLCSRVVRFADGRSLEDVILADALGLLGELPALDARAAGVWMM
                     QSPRRGRFESMRGVASACEVPGIEEVIVAARPGQVLEPLPEGFLYTGFVFARAQTPDA
                     VEHALRTAFARLSPVIVGADNPA"
     gene            complement(3125..5728)
                     /locus_tag="VEC56_00700"
     CDS             complement(3125..5728)
                     /locus_tag="VEC56_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12698.1"
                     /translation="MLVAIAGAFLGAVAASSVRAEVDPDSLYLARHPSLLYGPADVPA
                     LRARVQAPGAPAAAYAFILDVVQTVYPTAPFDTLLHNDFAQEPIVNLGLAAYLNDPVD
                     PTALALGRDLTLHIARTFDVDDEAYGSALRLRSLAIGYDAYFGVATPIERAEIRAEAA
                     SYIAYITANMNYDVWLHRPYVSNKSAMLAAALGLAAISFADEIDPALIASAFARSDAL
                     YAAWRDAHLSDDACYREGSLYGLWSLRQLVYYFHARKRFDGTVYSGDWALRWMERWLA
                     YELDPRGGARMNNIQDQTDYFLPLARHTTYFDWAMSEWGSGLSRYLWDRASGPLGVDM
                     GDENDKAGTVLWHQDVASVNPGTLLPRSMLWESRGLYYYRSGWTDQAASDDISISFYC
                     GEFRGGHAQEDQTQFTLTAFGEKLVIDHGSGSMARQSEAHNIVRIDQAGQHNAGSSIG
                     TDGRIAESMLTDFADYVCGDATAAYTTYSPYNAAGVPYPWTDWSWGYTGANPVEHALR
                     RVLVVHDSDFPTYLIVQDDIDKDGEVHRYDWCVHFPEVATVDTSIPAGPIGVAVGNAR
                     LDVHAIFPLKPKLPGVTLFWFDNQSEDPDSRMLALTSFAVNPKFAVLLMPRRDTDPAP
                     LVFSSTFSAGTMSVVRAPSGAIDVVFARGNRVGPIAPMPLAELPDAEGCGSISIDAEL
                     AVVRLGSEGVSRYLAVDARSIACAAGDIFTLYDGPATIAFDGTRVHIDRPDATFRILA
                     DGVSEVVCRGEVVPTGLDGRYLVRVSATDVPAPPARDGLALRAFPNPFNPSARISFAT
                     PARGRVDAAVYDAAGRFVARLASGVFPAGEHDLSWDGRDHAGRAAASGVYFLRVRAGT
                     ARETLKLVLLR"
     gene            complement(5903..6247)
                     /locus_tag="VEC56_00705"
     CDS             complement(5903..6247)
                     /locus_tag="VEC56_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012089800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HXV12699.1"
                     /translation="MRTTLITIILLTASNVFMTFAWYGHLKHMSAKPWFIAALASWGI
                     AFFEYMIQVPANRIGYRVMDLAQLKLVQEVVTLSVFIPFAMLYMKERPRLDYLWAALC
                     LLGAVYFAFRKG"
     gene            complement(6244..6678)
                     /locus_tag="VEC56_00710"
     CDS             complement(6244..6678)
                     /locus_tag="VEC56_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12700.1"
                     /translation="MTSRALAYAWALPTTAIGLALVAVAMATGARAAVVDGVMEAHGG
                     LLDPLLRRAVPLRGGASAMTLGHVVVGRTPDALARTRAHERAHVRQAERWGPFFLPAY
                     LLASVIVSARGGHYYRDNRFERDAVAVAARANDPNQPGGART"
     gene            complement(6675..8318)
                     /gene="mtgA"
                     /locus_tag="VEC56_00715"
     CDS             complement(6675..8318)
                     /gene="mtgA"
                     /locus_tag="VEC56_00715"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXV12701.1"
                     /translation="MIGYLVRKVRRHQAKRTFREYGFDVRRFQLPRDGEIEYAQWKHP
                     FEKPKVITQDDVDGLRRFIRPGDTAIDVGAHTGDTTVPMALACGSDGLTIALEPNPHV
                     FKILEENARRNLDKTHIAPLNFAATEDDGDFQFHYWDASFGNGGYLSRLRNQRHGHRF
                     PLRVKGRNLERYLRTYHADRLPRLAFLKTDCEGYDKDVLRSLRGVLKQYRPVVVCEVH
                     KKLDGSERHELFDALKEAGYALHTYLGGANPVGEALARDDLAKRPRLDVIALPEEVDR
                     AAWNHEGWAARAPAVPAAVDEGARAAPAPPRVERRATPAGRAGRVLRVAAWVFVGWIA
                     LTVTPVIALRWVDPPTSAFMVQRRIEGIVNPTKRVSIRYQWTDWDRISKNTRLAMIAA
                     EDQRFVEHDGFDFEAIEKAREQNRRRGRLRGASTISQQVAKNLFLWPGRSWFRKGLEA
                     GYTILIEALWPKRRVLEVYLNIAEFGDGVYGVGAASRRFFDTSPARLSRKESALLAAV
                     LPNPKRFRVDRPSKYVQRRAWWIERQMIRLGPAALGPLS"
     gene            complement(8335..8949)
                     /locus_tag="VEC56_00720"
     CDS             complement(8335..8949)
                     /locus_tag="VEC56_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12702.1"
                     /translation="MRKSGLVAAVVLAALAGACGNTQVLDSWTDSAVTPAPVQRIAVV
                     GLSGDEVTRRLFEDTFGGELKKRGNDAVAGHTFLPKELEGDPDAALALITKEGFDAVL
                     TARSLGVKTEETVTQGSAYYIPDSGYYGWNGYYGAVYQDITATTYTTRTEKAMVETHL
                     YQASTKKLVWAARSATTSTGKMTEGMRDYARTVVRNLAKSGFIK"
     gene            complement(8961..10562)
                     /locus_tag="VEC56_00725"
     CDS             complement(8961..10562)
                     /locus_tag="VEC56_00725"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXV12703.1"
                     /translation="MKTRFRIVRAMIPAVFVWLALTAPSLGAESDEPAVIRFHNRDIA
                     TLRATLGPVDPQERAAAALERLRSSTPGDLRRPVTVVPFEESRALLLGDRILFVLVTG
                     DVDVSAGETVNEAAEQAAANLRVAFASWADQRRPEVVARGVIHVLVATAIGLVLLFLL
                     LRLRRFLLNRLIPRAERAIEKRRFVVFGQDLRRHALNALRISIGALYAISVVVVVYSW
                     LMYSLRQFPLTQPWGDGLADFLLDTLGMIGVGILHGIPEMVVVVLVLVVTRFFARVVA
                     GIFDAVERGQVTIRGVHPDTADATRRIATVLVWIFGIAIAYPFIPGSQSDAFKGISVL
                     VGLMVSLGSSGVISQAMSGMVVVFSRALKAGEYVCIGDYEGTVSEVGALSTKLRTVRN
                     EEINIPNAMLVSSTTKNYSRLAKERGIIVYATAGIGYDAPWRVVHRLLIDAALRTPGI
                     KHEPAPFVRQSALGSFAVDYTINAYLERAEDRLPVLSDLHANIQDGFNEAGIQIMTPA
                     FESQPAEPVVVPKSMWGEGEGRNPR"
     gene            10680..12515
                     /gene="typA"
                     /locus_tag="VEC56_00730"
     CDS             10680..12515
                     /gene="typA"
                     /locus_tag="VEC56_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXV12704.1"
                     /translation="MPNQKRRKDVRNIAIIAHVDHGKTTLVDALLKQTGAHAFKEGEV
                     TILDSNPLEKERGITIFSKNASIRYKDLTINIVDTPGHADFGSEVERILRMVDGVLLL
                     VDAFDGPMPQTKFVLKKSLELHLKPIVVVNKVDRPHSRPMEVADLTFDLFCELNATDE
                     QLDFPIVYASGKLGWATMDLDEPATDMKALLETIVHRVLPPVAEVDHPFQMLVTMLDY
                     DSYVGRIGSGRIYHGAVKLGDPVVLIKRDGATARSKVTRILRYTGLKRSEVQAALAGD
                     IVSIAGVEDVHVGDTLASPERPEALPLIKVDEPTISMFFSHSTSPLSGKDGGRFLTSR
                     HIRERLEHEAMINVGIRVEDADGGERLKVSGRGELHLAILIETMRREGYEMEVSRPRV
                     ILKEVDGEVMEPVEEVVVEVDPGFEGVVIEALGKRKGEMKSMATSAVGTIRMEFIIAT
                     RGLIGFRSEFLTMTRGTGVMYQNFYEYQPYKGEMPQRNHGVQIALHPGTAVAYALWSL
                     EERGEILIRPGDTVYEGMIIGVNNKGSDLVVNAIKEKKLTNMRSSGADDAIQLTPPRE
                     MTLEFALEFIEDDELVEVTPKSIRLRKKILNDQERKVASKRPATV"
     gene            complement(12528..12845)
                     /locus_tag="VEC56_00735"
     CDS             complement(12528..12845)
                     /locus_tag="VEC56_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HXV12705.1"
                     /translation="MAWVHLFFAGVMEWGWPIGLKFAAQPQHRAWAIPLAVVSILLSG
                     WLLLLAQRTIPIGTAYAVWTGIGAVGTFVLGILVLGEPAQLARFFFVGLIVVGIMGLK
                     WVS"
     gene            complement(12847..13284)
                     /locus_tag="VEC56_00740"
     CDS             complement(12847..13284)
                     /locus_tag="VEC56_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12706.1"
                     /translation="MGRNIVAVVVGYVVMFVVVMATFTGVFLAMGADRAFQPGSYQVT
                     GLWIFVSTILSFAAAILGGFVAGRIGKGPRAAHLLAIVVVVLGVILAIPTLDAPEPGE
                     PRTGEVGNTDAMMKAQQPPVISFLNPVIGAVGALLGSRLRRPS"
     gene            13444..>14370
                     /locus_tag="VEC56_00745"
     CDS             13444..>14370
                     /locus_tag="VEC56_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12707.1"
                     /translation="MHRMRPRASTVVLSLTLLSTASFANDLTHFESPPVHPVELSPTG
                     NRLFVTHTADHRLVVFEVNASPPKKIAEVMVGLEPVTVRARTQSEVWVVNHVSDDISI
                     VDVLTGRVVRTLLVGDEPTDVVFAGNRAFVCVSQEDKLRVFDLTDLTLAPVDIPLEMS
                     DPRSLALSPDGSTVYVCALDGGNQTTLVPTEVVSANGGLPTPNPPMNPALAAAPDVGL
                     IVKHNGSYWFDEIGRNWNAFVPYTLLDNDVVALSTSSLAVVASYRNVGTTLFNVDVHP
                     NGNLYVTNQEAFNEVRFEPNVRGKFVRNRVTVV"
BASE COUNT         2417 a         4930 c         4733 g         2290 t
ORIGIN      
        1 cgcggagctc aatgcggatc acctcttgcc cgctctcgcg gactcgtgcc tgggcgccga
       61 gttcgagact ccgctcgtgg agctcgcgcg tcgcatcgcc gggacgcgcg cgaccggcgc
      121 ccacccgcgt gacgcggcac cgcagcgagc gatcgcggcg agcgatcgct ctcccaggcg
      181 cgcgcttgat ctcacacccg cgcgcgaggg actgtcgcac cgcgagcgct acgcgaagct
      241 cgcgctgggc gacgagcgcg tcgaggtcgg atacgtcgcg accacgcgcg ggtgcaagca
      301 cacctgccgg cactgcccgc tcccgcccgc gtacgcaggg tcgttctacg cgctcccgat
      361 cgcgcccatc atggacgacg tggcccgcct ggtcgcacgc ggcgcgcgcc acgtcacctt
      421 cgccgacccc gactttttga atggccccac ccacgcgctg cgggtggcac gcgaggtcgt
      481 gcggcggttt cccggcgtga ccttcgatta caccgccaag atcgcgcacc tcctccgcca
      541 cgacgaggtc gtggacgagc tgcacgaact gggcaacctg ttcgtcgtgt cggcggtcga
      601 gtcgttcaac gatgcggtgc tggcggcgct gcaaaagggg cacacccgcg ccgacgcgct
      661 gtccgtcgtg cggcgttttc gcgcgcgcgg cctcacgctc cgccccacct tcgttccgtt
      721 tacgccgtgg gagacgcggg aaagctacac cgagatgttc gacatcgtcg agggcgaagg
      781 actcgtcggc cacatcgaac ccgtgcagta cgcgattcgg ttgctcgtcc ccgaacgctc
      841 gctgttgctc ggcgcggccg agatgcgccc acacctgggc ggcttcgaca cgcttacgtt
      901 ctcctacgcg tggcaccacc ccgatcccgc gatggacgac ctgcagcgca cggtcgcgcg
      961 caccgcggcc gacttggtgg gggcgggtgt ggaccacgac cacgccttcc tgcgcctgcg
     1021 cgaggcggcc ggcgtcacgc gggcgcgggg accggcgcgg gcccgcgcgg ggcgcgcgcc
     1081 gcgcctcacc gaagactggt tctgctgagc ggagcccacc gagggccagt tgggctctct
     1141 ccacgcgtgg acccggcatg ccggcggcga gggaacgctt gcgtgatcgt cgcgcgttgc
     1201 ctatgatcga ctcgccgtca tggacgaact gaccttcaat ttcgaactgc ccgaggggcg
     1261 ctcggtgcgc atcaccgcga caccgtcgat gatgcacccg gagcagtgca ccttcacggt
     1321 cagcgagccg gtgtacccgg gccgctccgc gtacttcggc gatcgcgacc agtcgcgcga
     1381 gtcggtcctc gtcgatcgcc tgttcgcgat cgaaggcgtg gccgacgtgc tcgtcaacca
     1441 cgacacggtg cgcgtgacgc tcgtcggcga tgcgaactgg gaggacaagg cgccccgcgt
     1501 cggcgccgcc attcgcgacg gcctcgcggc cggagccccc atttcccagg cggtcaccga
     1561 gagcctgctc gacccgcagg agacgcgccg ccgcgtccag caggtgctcg acaccgtcat
     1621 caaccccgcc gtcgcgagcc acggaggcgt cgttcgattg ctggacgtgg ccaacaacac
     1681 ggtgttcctc gaattcggcg gcggttgcca gggctgtggg atggtcagcg tgaccctcaa
     1741 atacggcgtc gaacgcacca tccgcgacga agtccccgaa gtcggcgcca tcatggacac
     1801 caccgaccac gccgccggtc gcaacccgta ctacgccccg tcttccaagt aaacgccccg
     1861 cgctcgttct cgtcgcctcg atgaaaccgc gcctgctctt gctggttccg acctcgacgt
     1921 atcgcgccga ggcgttcatc cgggccgcgc agcgcttgcc catcgacttg agcatcgcgt
     1981 cggaatcacc caactcgctc tcgcatctcc acccggtcga tctcccgacc ttcgatttca
     2041 ccagcccggc ggacgccgcc gcgcgcgcgt acgcgctcgc caagacccat cccgtgcacg
     2101 ccgtggtcgc ggtcgacgac caggccacgc tggcggcggc ggtgatctcg gaagccctcg
     2161 gactcgcgca caatccggcc cacgccgtgc gcgcggccct cgacaagtat cgcgcacgcg
     2221 agcgcatgcg ggccgtgggc atcgcccagc ccgattatcg tctggtgtcg ttcgacggtt
     2281 acgtcgacgc ggcccagtcg ctctcgtacc cggccgtggt caagccgctc gcgatggcgg
     2341 ccagccgcgg cgtcgttcgg gtcgacaacg cgtcgcagtt cgtcgacgcc ttcgcgcgcg
     2401 ttgcctcgtt ggtgtcgtcc aacgcatcgc acctcgacga gtcggcgcga tcgcacttgc
     2461 tggtggaggc gtacgtgccg ggctgggagg tcgcggtgga agggctcgtg gtcaaggggc
     2521 gggcgcgcgt cttcgccgta ttcgacaaac ccgacccgct ggaaggacca tattttcccg
     2581 agacgatgta cgtgacaccg tcgcggctcg gcgatgcgac ggtcgaccga atcgtccggc
     2641 tgaccgcaga ggcggtgcac gcggtcgggc tcacgcacgg gcccttccac gtcgagatgc
     2701 gcggcgatgg cgaccgcctg ctgccgatcg aagtgcacgc gcgctccatc ggcggcttgt
     2761 gctcgcgcgt ggtgcgcttc gccgacgggc gctcgttgga ggacgtcatc ctcgcggacg
     2821 cactcggcct cttgggcgaa ctgcccgccc tggacgcgcg cgcggccggg gtgtggatga
     2881 tgcagtctcc gcgccgcggg cgcttcgaat cgatgcgcgg cgtggcgtcg gcgtgcgagg
     2941 tcccgggaat cgaagaagtg atcgtcgcgg cgcgaccggg gcaggtgttg gagccgctgc
     3001 ccgaggggtt tctctacacc ggcttcgtct tcgcgcgcgc gcaaacgccc gatgcggtcg
     3061 agcacgcgct gcgcaccgcg ttcgcgcgcc tctcccccgt catcgtcggc gccgacaacc
     3121 cggcctagcg gagcaagacc agcttcagcg tctcgcgcgc ggtccccgcg cgcacgcgca
     3181 ggaagtacac gccgctggcg gccgcgcgcc ccgcgtggtc gcgaccatcc cacgaaagat
     3241 catgctcgcc ggcggggaac acacccgacg ccagcctcgc gacgaagcgc cccgccgcgt
     3301 cgtagaccgc cgcgtccacg cgtccccgcg ccggggttgc gaacgagatt cgcgccgatg
     3361 gattgaacgg gttgggaaac gcgcgcaagg cgaggccgtc gcgcgccggc ggcgccggta
     3421 cgtcggtggc ggagacgcgc accaggtatc tcccgtccag gccggtgggc acgacttccc
     3481 cccggcacac cacttcgctg actccgtcgg cgaggatgcg gaacgtcgcg tccgggcggt
     3541 cgatgtggac gcgtgtcccg tcgaaggcga tggtcgcggg tccgtcgtag agggtgaaga
     3601 tgtcgcccgc cgcgcacgcg atcgatcgcg cgtcgacggc gaggtagcgc gaaacccctt
     3661 cgctccccaa tcgaacgacg gccagctcgg cgtcgatgga gatggagccg cagccctccg
     3721 cgtccggaag ctccgcgagc ggcatggggg cgatcggacc cacgcgattg ccgcgcgcga
     3781 acacgacgtc gatcgcgccc gagggcgccc tgaccacgct catcgttccc gcggagaagg
     3841 tcgacgagaa caccaggggg gcggggtcgg tgtcgcgccg cggcatcaag agcaccgcaa
     3901 acttcggatt cacggcgaac gacgtgagcg ccagcatccg cgagtccggg tcctccgact
     3961 ggttgtcgaa ccagaacaac gttacgccgg ggagtttcgg cttgagcggg aagatggcgt
     4021 gcacgtcgag gcgggcgttg ccgaccgcga cgccgatggg cccggccggg atcgacgtgt
     4081 cgacggtcgc tacctcggga aagtgcacgc accagtcgta gcggtgcacc tcgccgtcct
     4141 tgtcgatgtc atcctggacg atcaggtagg tgggaaagtc gctgtcgtgc acgaccagca
     4201 ctcggcgcag cgcgtgctcc accgggttgg ctccggtata cccccacgac cagtcggtcc
     4261 acgggtacgg gacgccggcc gcgttatagg ggctgtaggt ggtgtacgcc gcggtcgcgt
     4321 cgccgcacac gtagtccgcg aagtcggtca gcatcgactc ggcgatgcgg ccgtcggtgc
     4381 cgatggaact gcccgcgttg tgctgccccg cctggtcgat gcgcacgatg ttgtgtgcct
     4441 cgctctgccg ggccatgctc ccggagccgt ggtcgatcac caacttttcg ccgaacgcgg
     4501 tcagcgtgaa ttgggtctgg tcctcttgtg cgtgcccgcc gcgaaactcg ccgcagtaga
     4561 aactgatcga gatgtcgtcg gaggcggcct ggtcggtcca gccggagcgg tagtagtaca
     4621 gcccgcgcga ctcccacagc atgctccggg gcagcagcgt gcccggattc accgacgcca
     4681 cgtcctggtg ccagagcacg gtgcccgcct tgtcgttctc gtcccccatg tcgaccccca
     4741 gcggtcccga cgcgcgatcc cacaggtagc gcgacagacc gctcccccac tccgacatcg
     4801 cccaatcgaa atacgtggtg tggcgcgcga gcgggagaaa gtaatcggtt tgatcctgga
     4861 tgttgttcat gcgcgcgccc ccgcggggat cgagctcgta cgccaaccat cgctccatcc
     4921 atcgcaaggc ccagtcgccc gagtacaccg ttccgtcgaa gcgcttgcgc gcgtggaagt
     4981 agtagacgag ttggcgcaga ctccacagtc cgtagagcga cccttcgcga tagcacgcgt
     5041 cgtcggacag gtgcgcgtcg cgccacgcgg cgtagagcgc gtcggagcgc gcgaacgccg
     5101 atgcgatcag ggccgggtcg atctcgtccg cgaacgagat cgcggccaag ccgagggccg
     5161 cggcgagcat cgccgacttg ttgctcacgt acggccggtg cagccacacg tcgtagttca
     5221 tgttcgcggt gatgtaggcg atgtaactcg ccgcctcagc gcggatctcc gcgcgctcga
     5281 tcggggtcgc cacgccgaag tacgcgtcgt agccgatggc gagcgagcgc aggcgcagcg
     5341 cgctgccgta ggcctcgtcg tcgacgtcga aggtgcgcgc gatgtggagc gtgaggtcac
     5401 gcccgagcgc tagcgcggtg gggtcgacgg gatcgttcag gtaggcggcg agccctaggt
     5461 tgacgatcgg ctcctgcgcg aaatcgttgt ggagcagcgt gtcgaaggga gcggtcgggt
     5521 acacggtttg gaccacgtcg agaatgaagg cgtaggcggc ggcgggagcc cccggggcct
     5581 ggacccgggc ccgcagggcc ggcacgtcgg ccggaccgta gaggagcgag ggatggcggg
     5641 cgagatagag ggagtcggga tcgacttcgg cgcgcacgga cgacgccgcg accgctccga
     5701 ggaacgcccc cgcgattgcg accagcaacc gcgcgcgtgg gaggatccga accgtgatgg
     5761 gcatcccgta cgtgggtgcc cggcttcgtg ccggggcttc gactacgtgt ttctcgtggg
     5821 cccgcgggcc gtcccccatc gaccctgtaa ccgtagtagt gtatggcagt tcgacaacgt
     5881 ggtcaaaccg aattcgaggg ccttacccct tccggaaggc gaagtagacc gcacccagca
     5941 ggcacagagc ggcccacagg tagtccaggc gcggtcgctc cttcatgtac agcatcgcga
     6001 aggggatgaa caccgagagt gtcacgacct cttggaccag cttgagctgg gcgaggtcca
     6061 tcacgcggta gccgatccgg ttggcgggaa cctggatcat gtattcgaag aaggcgatcc
     6121 cccagctcgc cagcgcggcg atgaaccacg gcttcgcgct catgtgcttg aggtggccgt
     6181 accacgcaaa cgtcatgaag acgttggacg cggtgagcag gatgatcgta atcagtgtcg
     6241 tcctcacgtg cgtgctcctc cgggctggtt ggggtcattg gcgcgtgccg cgaccgcgac
     6301 cgcgtcgcgc tcgaatcgat tatctcggta gtagtgcccg ccgcgcgccg acacgatgac
     6361 cgaggcaagc aagtatgcgg gcaggaagaa cggcccccaa cgctcggcct ggcgaacgtg
     6421 ggcgcgttcg tgcgcgcgcg tgcgcgcgag cgcgtcgggg gtacggccga cgacgacgtg
     6481 cccgagcgtc atcgcactcg cgccgccgcg cagcggtacc gcgcggcgca agagcggatc
     6541 gagcagaccg ccgtgcgctt ccatgactcc gtccacgacc gcggcgcggg cgcccgtggc
     6601 catcgccacg gcgaccaacg ccagcccgat cgcggtggtc gggagggccc acgcgtacgc
     6661 gagcgcgcgc gacgtcacga cagcggaccc agcgccgccg ggcccaggcg gatcatctgc
     6721 cgctcgatcc accacgcgcg gcgctggacg tacttcgagg gccggtcgac gcgaaatcgc
     6781 ttggggttgg gcagcaccgc ggccaagagc gcgctctcct tacgcgatag ccgcgcgggc
     6841 gacgtgtcga aaaagcgtcg cgacgccgca cccacgccgt acactccgtc gccgaactcg
     6901 gcgatgttga ggtacacctc gagcacacga cgcttgggcc acagcgcctc gatcaagatg
     6961 gtgtaccccg cctccagtcc cttgcgaaac cagctccggc ccggccacag aaacaagttc
     7021 ttcgccactt gctgcgagat ggtgctcgcg ccgcgcaagc gcccgcggcg ccggttctgc
     7081 tcccgcgcct tttcgatggc ttcgaagtcg aagccgtcgt gctcgacgaa gcgctggtct
     7141 tcggcggcaa tcatcgccaa ccgcgtgttc ttggagatgc ggtcccagtc ggtccactgg
     7201 tagcgaatcg agacgcgctt ggtcgggttc acgatgccct cgatgcgtcg ctgcaccatg
     7261 aatgcggacg tcggggggtc gacccagcgc aacgcgatga ccggtgtcac ggtgagcgcg
     7321 atccacccca cgaacaccca cgccgcgact cggagcacgc ggcccgcgcg ccccgcgggt
     7381 gtcgcgcggc gctcgacgcg gggcggtgcg ggcgcggcgc gcgcgccctc gtcgacggcc
     7441 gcgggaaccg ccggcgcgcg ggcggcccac ccctcgtggt tccacgcggc gcggtccact
     7501 tcctccggca gcgcgatcac gtccaggcgc gggcgcttcg cgaggtcgtc gcgcgccagc
     7561 gcttcgccca ccgggttcgc accgccaaga tacgtatgca gcgcgtatcc ggcttccttc
     7621 agcgcgtcga acaactcgtg tcgttccgac ccatcgagct tcttgtgcac ctcgcacacc
     7681 acgacgggtc ggtattgttt gaggacgccg cgcagcgacc gcagtacgtc cttgtcgtag
     7741 ccctcgcaat cggtcttgag gaacgcgagc cgtggcagtc gatcggcgtg gtaggtgcgc
     7801 aggtaccgct cgaggttgcg ccccttgacg cgcaggggaa aacggtggcc gtggcgctgg
     7861 ttgcgaagcc gggagagata gccgccgttt ccgaacgacg cgtcccagta gtggaactgg
     7921 aaatcgccgt cgtcttcggt ggcggcgaag ttgagcggcg cgatgtgcgt cttgtcgagg
     7981 tttcggcgcg cattctcttc gaggatcttg aagacgtggg ggttgggctc gagcgcgatc
     8041 gtgagcccgt cggaaccgca cgcgagtgcc atgggcaccg tggtgtcgcc ggtgtgcgcg
     8101 cccacgtcga tcgcggtgtc gcccgggcga atgaaccgcc gcagcccgtc gacgtcgtcc
     8161 tgggtgatga ctttgggctt ctcgaacggg tgcttccact gcgcgtactc gatttcgccg
     8221 tcgcgcggaa gctggaagcg acggacgtcg aagccgtact cgcggaaggt gcgcttcgct
     8281 tggtggcgcc gaaccttccg caccaggtat ccgatcatgg gcgccgcaac cgcctcactt
     8341 gatgaagccg ctcttggcca gattccggac cacggtgcgc gcgtagtcgc gcatcccctc
     8401 ggtcatcttc ccggtgctgg tagtcgcgga gcgcgcggcc catacgagct tcttggtcga
     8461 cgcttggtac aaatgggttt ccaccatcgc cttctcggtg cgcgtggtgt acgtggtggc
     8521 cgtgatgtct tggtagaccg ccccgtagta gccgttccat ccgtagtagc ccgagtcggg
     8581 aatgtagtac gcgctcccct gcgtgaccgt ctcctcggtc ttgaccccga gcgagcgcgc
     8641 ggtcaggacc gcgtcgaagc cttccttcgt gatcagtgcc aacgccgcgt cgggatcccc
     8701 ctcgagttcc ttgggcagga aggtgtgccc cgccacggcg tcgttgccgc gcttcttcag
     8761 ctccccgccg aaggtgtcct cgaagagacg gcgcgtcacc tcgtcaccgc tcaacccgac
     8821 caccgcgatg cgctggacgg gggcgggcgt caccgcggaa tcggtccacg aatcgagcac
     8881 ctgcgtgttt ccgcacgcac cggccagcgc cgccagcacc accgccgcca ccaatccact
     8941 ctttctcatt gggtcctcca tcatcgcggg tttcgcccct cgccctcgcc ccacatggac
     9001 ttgggcacca ccaccggctc ggcgggctga ctctcgaacg ccggcgtcat gatctggatg
     9061 ccggcctcgt tgaacccgtc ctgaatattg gcgtgcaaat cggacaacac cggcaggcgg
     9121 tcctccgcgc gctcgaggta cgcgttgatc gtgtaatcga ccgcgaaact cccgagcgcg
     9181 ctttgccgca cgaacggcgc gggctcgtgc ttgatccccg gcgtgcgcag cgcggcgtcg
     9241 atcaggagcc ggtgcacgac gcgccagggg gcgtcgtaac cgattccggc cgtcgcgtac
     9301 acgatgattc cgcgctcctt tgccagtcgc gagtagttct tggtcgtgct cgataccagc
     9361 atcgcgttgg gaatgttgat ctcctcattg cgcacggtgc gcagcttggt cgacagcgcg
     9421 cccacttcgg acaccgtgcc ctcgtagtcg ccgatgcaca cgtactcgcc cgccttgagc
     9481 gcgcgcgaga agaccaccac catcccgctc atggcctggc ttatgacccc cgaagaaccc
     9541 agcgagacca tgaggcccac cagcacgctg atccccttga acgcgtcgct ttgcgagccc
     9601 gggatgaagg ggtacgcgat cgcaatgccg aagatccaca ccaataccgt ggcgatgcgg
     9661 cgtgtcgcat ccgccgtgtc ggggtggacg ccgcgtatcg ttacctggcc gcgttccacc
     9721 gcgtcgaaga tacccgcgac gacgcgggcg aaaaagcgcg tcaccaccag cacgagcacg
     9781 accacgacca tctccgggat accgtggaga atcccgaccc cgatcatccc caacgtgtcg
     9841 agaagaaagt ccgccaggcc gtctccccag ggctgcgtca gcgggaactg gcgcagcgaa
     9901 tacatgagcc acgagtagac gacgacgaca acggagatcg cgtacagggc cccgattgat
     9961 atgcgaagcg cgttgagcgc gtggcggcgc aagtcttgcc caaagaccac gaagcgccgc
    10021 ttctcgatcg cgcgctcggc gcgcgggatg agacggttca agaggaaccg gcgcaggcgc
    10081 aggagcagga agagcagcac aagtccgatg gcggtcgcga ccagcacgtg gatcacgccg
    10141 cgcgcgacga cttcgggacg acgctggtcg gcccacgaag cgaatgcgac gcgcagattg
    10201 gcggcggcct gctcggccgc ctcgttcacg gtttctcccg cgctcacgtc gacgtcgccg
    10261 gtgacgagca cgaagaggat gcgatcaccc aagagcagcg cgcgcgactc ttcgaaagga
    10321 accaccgtga ccggtcgacg aaggtcgccc ggagtgctgc tccgcaaccg ctccagcgcg
    10381 gctgcggctc gttcctgcgg gtcgaccggg cccagggtcg cgcgaagggt ggcgatgtcg
    10441 cgattatgga agcgaatgac ggcgggctcg tcggattccg cgcccaggga cggagccgtg
    10501 agcgcaagcc acacgaaaac cgccggtatc atggcacgga cgattcggaa tcgggtcttc
    10561 atagggttcg gtaacgtcct ttcggcccca aggcggcgaa acccgtcatt ttccatcgtt
    10621 gcagccgggc cgggtttggc ctatattacg tcgcccgcgc cggaaagcgc aacaagacca
    10681 tgcccaacca gaaacgacgc aaagacgtcc gcaacatcgc gatcatcgcc catgtcgacc
    10741 acggcaagac caccctggtc gacgcgctgt tgaagcagac gggcgcccac gccttcaagg
    10801 agggcgaggt cacgatcctc gactccaacc ccttggagaa ggagcgcggc atcaccattt
    10861 tctcgaagaa cgcgtccatc cggtacaagg acctgaccat caacatcgtc gacacccccg
    10921 gccacgccga cttcggcagc gaggtcgagc gcatcctgcg catggtcgac ggggtgctct
    10981 tgctcgtgga tgctttcgac ggccccatgc cccagaccaa gttcgtgctc aagaaatccc
    11041 tcgagctgca cttgaagccg atcgtcgtcg tcaacaaggt cgaccgtccg cattcgcgcc
    11101 cgatggaagt cgccgatctg accttcgatc tcttttgcga attgaacgca accgacgagc
    11161 agctcgactt ccccatcgtc tacgcgtccg gcaagctggg ctgggcgacc atggatctcg
    11221 acgagcccgc gaccgatatg aaggcgctct tggagaccat cgtgcaccgc gtgcttccgc
    11281 ccgtggccga agtcgaccac cccttccaga tgctggtcac catgctcgat tacgattcgt
    11341 acgtgggccg gatcggcagc gggcgcatct atcacggcgc ggtcaagctg ggcgatccgg
    11401 tcgttctcat caagcgcgat ggcgccacgg cgcgctcgaa ggtcacgcgc attctccgct
    11461 acaccggact gaagcgctcc gaagtacagg cggcgctggc gggcgacatc gtatccatcg
    11521 cgggggtcga agacgtccac gtgggcgata ccctggcctc gcccgagcgc ccggaagcgc
    11581 tgccgctcat caaggtcgac gagcccacca tttcgatgtt cttctcccac agcacgagcc
    11641 cgctgtccgg gaaggacggc gggcgcttcc tgacctcgcg ccacattcgc gagcgtctgg
    11701 agcacgaggc gatgatcaac gtcggcatcc gcgtcgaaga cgccgacggc ggcgagcgcc
    11761 tgaaggtctc cggccgcggc gagttgcacc tggccatctt gatcgagacc atgcgtcgcg
    11821 aaggctacga aatggaagtc tcgcgcccgc gcgtcatctt gaaggaagtc gacggcgagg
    11881 tcatggagcc ggtcgaggaa gtcgtggtgg aagtcgaccc cggattcgag ggcgtcgtca
    11941 tcgaagccct cggcaagcgc aagggcgaga tgaagtcgat ggcgaccagt gccgtgggga
    12001 ccattcgcat ggagttcatc atcgcgacgc gcgggctgat cggtttccgc agcgagttcc
    12061 tcacgatgac gcgcggcacc ggcgtcatgt accagaattt ttacgagtat cagccgtaca
    12121 agggcgagat gccgcagcgc aaccacgggg tccagatcgc cctccacccc ggaaccgccg
    12181 tggcgtacgc actgtggagc ctggaagagc gcggcgagat cctcattcgc cccggcgaca
    12241 ccgtctacga gggcatgatc atcggcgtca acaacaaggg ctccgacttg gtcgtcaacg
    12301 cgatcaagga gaagaagctc accaacatgc gctcgtcggg cgccgacgac gccatccagc
    12361 tgacaccgcc gcgcgagatg acgctcgagt tcgcgctcga gttcatcgaa gacgacgaat
    12421 tggtggaagt gacgccgaag tcgatccggc tgcgcaagaa gatcctcaac gaccaggaac
    12481 gcaaggtcgc gtccaagcga ccggcgacgg tgtagcgtag ggggaactca agagacccac
    12541 ttgagcccca tgatcccgac cacgatcaag cccacgaaga agaagcgcgc gagctgcgcg
    12601 ggctcgccca gcaccagaat ccccagcacg aaggtcccca ccgcgccgat gccggtccac
    12661 accgcgtagg cggtcccgat cgggatggtg cgctgcgcca agagcagcaa ccagccactc
    12721 agtagaattg agacgacggc gagcgggatg gcccaggcgc ggtgctgggg ctgcgcggcg
    12781 aacttgagcc cgatcggcca gccccactcc atcacaccgg cgaagaacaa gtggacccac
    12841 gccatgctac gacggccttc gcaagcggct gccgagcagg gcgcccaccg caccgatcac
    12901 cgggttcaag aatgaaatga cgggaggctg ctgggccttc atcatggcgt ccgtattgcc
    12961 cacctcgcct gtgcggggct cgcccggctc gggcgcatcg agggtcggaa ttgcgaggat
    13021 gacgccgagc acgaccacga cgatggccag gagatgtgcg gcgcgtggtc ccttgccgat
    13081 gcgcccggcc acgaagccgc ccagaatcgc ggcggcaaaa ctcaagatcg tgcttacaaa
    13141 aatccaaagc ccagtgactt ggtagctccc gggctggaac gcgcggtccg cccccatcgc
    13201 caagaacacg cccgtgaagg tcgccatcac gaccacgaac atcaccacgt accccacgac
    13261 gacggccacg atgtttcgtc ccatcgggac acctcctcgc ggcgctgcgc cgcgcgcaca
    13321 gggtaccgcg cctttcgccg gaggcgcaac cgcgattgag cccgcagcgt cgccatgcta
    13381 caatgatggc ctctttttcg acgaccacgc gcttcactcc catcgtctag gagacgcaac
    13441 ccgatgcatc gcatgcgacc ccgggcatcg accgttgtat tgtcgttgac cctgctttcc
    13501 acggcttcgt tcgccaacga cctcacgcat ttcgagagcc cgcccgtgca tccggtggag
    13561 ctgtctccaa ccggcaatcg cctgtttgtc acccacaccg cggatcaccg cctggtggtg
    13621 ttcgaggtca acgccagccc gcccaagaag atcgccgagg tcatggtggg cttggagccg
    13681 gtgacggtcc gcgcgcgcac gcaaagcgaa gtgtgggtgg tcaatcacgt ctccgacgac
    13741 atcagcatcg tcgacgtctt gaccgggcgc gtcgtgcgta cgctgctggt gggcgacgaa
    13801 cccaccgacg tcgtcttcgc gggcaaccgc gccttcgtgt gcgtgtcaca agaggacaag
    13861 ctgcgcgtct tcgatctgac cgacctgacg ctggcgccgg tcgacattcc gctcgagatg
    13921 agtgacccgc gctcgctggc gctctcaccc gacgggtcca ccgtgtacgt gtgcgcgctc
    13981 gacggcggca accagaccac cttggtcccg accgaagtgg tgtcggccaa cgggggtctc
    14041 cccacgccca acccgcccat gaaccccgcg ctcgccgcgg ccccggacgt gggcctgatt
    14101 gtgaagcaca acggctcgta ctggttcgac gagatcggcc gcaattggaa cgccttcgtt
    14161 ccctacacgc tcctcgacaa cgacgtggtc gcgctcagca cgtcgtcgct cgccgtcgtc
    14221 gccagctacc ggaacgtcgg gacgacgttg ttcaacgtcg acgtgcaccc gaacggcaat
    14281 ctctacgtca ccaatcaaga ggcgttcaac gaggtccgct tcgaacccaa tgtgaggggc
    14341 aagttcgtcc ggaatcgcgt gacggtggtc
//
LOCUS       DASPGK010000029         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_944478, whole genome shotgun sequence.
ACCESSION   DASPGK010000029 DASPGK010000000
VERSION     DASPGK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944478"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2219
                     /locus_tag="VEC56_00750"
     CDS             <1..2219
                     /locus_tag="VEC56_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12708.1"
                     /translation="AVLAAAGDDANAAVTGGVTPTDPGEGNYVLVPADTLSGTPAFYR
                     FDELLGEFLVSFVEVAANTGDYRRAGISSQGTPYFEFVGATLGSYVVGRRLPLPTSVD
                     VATARVARETGAFTFDAEWNVSQFDQNLLSSLDDDDNVGSAGQVRAGLESEGDWRLGV
                     SALASVLEDRFRSFDRARPAFFYRDWNLEDVDLVGREATQEVSLSAARTTAGSARVTA
                     SHLDRDDFEGWKQEGVLAAGRLDDRGLAARAFYTDTEGEANQRTRRHLTTEAAYGVWK
                     VVPGITYGNENYRNAFDAAPDSGRAYELVGAHLSSRGAPRLAWRLAGERRDTRSVEAA
                     SDDFVDARRDITLSGSLVYRSTGAARAELQLIHRREDNLIVGGEAKTDLARLKAGSAW
                     DGIGLRADADYEVSQNDAATLQRSVVFVGEGKGDYNAIGEAVGKGKGAYTVVFLPTTD
                     TTPVHSVGFNLRVSWKPSQRPHTVGGAGGWILRNVSVDQTLGVREESTYEPAWEVYLM
                     LPSALQRDDATVFGATTFRQDWSLLDDYKNVSLTVRYLREDREDNRFEGVHESAQSGE
                     RAARLSRSLSAVLTGVLEGGQRVDRRGGEGIPGGTGSSYDVEDWFGLVGAGIVLAPGA
                     NLDVDVRAATLSDAESGAEQRSLKVTPRLVWRIADQLNLFGTYELAQVTDQNQTGVRP
                     LVFARDGTSQRWSITPNIRISNIIGIFATYSGRNERVFTGQRVVEHEFRLETRAYF"
     gene            2219..>3704
                     /locus_tag="VEC56_00755"
     CDS             2219..>3704
                     /locus_tag="VEC56_00755"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXV12709.1"
                     /translation="MTRRTGIIVTVLALFAALPPIALAADGAPARTTKLRVLGTHALP
                     GERAEALLRENGFPAARALRILQEAYIGEGYLAASIAVTVEADSSFTVVIDEGQRPRV
                     GSARANGLRARTEGDVLAALELTGGSPFEPKRLARNIDALLASYDNEGYPFAQVWLDS
                     LGLSADATTVAVSLTVVEGEPRAIEGVVVEGLQKTRPALAQRIAALETGVPYRAQALE
                     DAYLRMVASGVFTDVEFPTVRLSSDGRGVDAVITVVEPKRSHTFAAALGYASSDGTRD
                     RVLSGLVQLDLNNVGGTLKDFGASWNNDGVGRSETRIAYRDRLFLGRRLAVGLQLEQV
                     GQDTVYTWQSADLDVERGVGRVAGMLTSVSVGFAGDRNVYSVGDLVNSTRLRARAGAS
                     VLAGSERRAAFARVGVSATLAQKDLSYREGATGPGDVSQWIYDGRFDLLVPAFWSLYY
                     SLEGRAQTLESDEATVPLPEQFYLGGARTVRGYRENQFHGRRVAF"
BASE COUNT          562 a         1240 c         1359 g          543 t
ORIGIN      
        1 gcgccgtgct ggccgcggcc ggcgatgatg cgaacgcggc cgtgaccggc ggcgtcaccc
       61 cgaccgaccc gggtgagggc aattacgtcc tggtccctgc cgacacgctg tcgggaaccc
      121 ccgccttcta tcgcttcgac gaactgctgg gcgagttcct ggtgtccttc gtcgaagtcg
      181 ccgcgaatac cggcgattac cgtcgcgcgg gcatctcgtc gcagggtacg ccgtacttcg
      241 agtttgtggg cgccaccctc ggcagctacg tcgtcgggcg caggctgccg ctaccgacgt
      301 cggtcgacgt ggccaccgcg cgcgtcgcgc gcgaaacggg cgcgtttacc ttcgacgccg
      361 agtggaacgt gagccagttc gaccaaaacc tgctctcgtc cttggacgac gacgacaacg
      421 tcggcagcgc ggggcaggtg cgggcggggc tcgagagcga gggtgactgg cggctgggcg
      481 tttcggcgct cgcgagcgtg ctcgaagatc gcttccggag cttcgatcgc gcgcgaccgg
      541 cgttcttcta ccgcgactgg aacctcgagg acgtcgacct ggtggggcgc gaggccaccc
      601 aggaggtcag cctgagtgcg gcgcgcacga cggccgggag cgcgcgtgtc accgcgagcc
      661 acctcgaccg cgacgacttc gaggggtgga agcaggaggg cgtgctggcc gccggccggc
      721 tcgacgaccg cggtcttgcg gcgcgcgcct tctacaccga caccgagggc gaggccaacc
      781 agcgcacgcg gcgccacctg accacggagg cggcgtacgg cgtgtggaag gtggtgccgg
      841 ggatcacgta cgggaacgag aactaccgga atgcgttcga cgcggccccc gacagcgggc
      901 gcgcgtacga gctcgtgggc gcacatcttt cgagtcgggg agcaccgcgg ttggcgtggc
      961 gcctggccgg cgagcgtcgc gacacgcgca gcgtggaggc cgccagcgac gatttcgtcg
     1021 acgcgcgccg cgatatcacg ctgagcgggt cgctcgtgta ccgctccacc ggggccgcgc
     1081 gcgccgagct gcagctgatc catcgtcggg aagacaacct gatcgtgggc ggggaagcca
     1141 agaccgacct cgcgcgcctc aaggccggca gcgcgtggga cggtatcggc ctgcgcgccg
     1201 acgccgacta cgaagtgagc cagaacgacg ccgccacgct gcagcgctcg gtcgtgttcg
     1261 tcggcgaagg caagggcgac tacaacgcga tcggcgaggc ggtgggcaag ggcaaaggcg
     1321 cgtacacggt cgtgttcctg cctaccaccg acaccacccc ggtgcactcg gtcgggttca
     1381 atctgcgcgt ttcgtggaaa ccctcgcagc gcccgcacac cgtgggcggg gcaggcggat
     1441 ggatcctgcg caacgtctcc gtcgatcaga ccctgggggt gcgcgaggaa tccacctacg
     1501 aaccggcgtg ggaggtatac ctcatgctcc cctcggcgct ccagcgcgac gacgccaccg
     1561 tgttcggcgc caccaccttc cggcaggact ggtcgctgct cgacgactac aagaacgtgt
     1621 cgctcacggt tcgctacctg cgcgaggacc gcgaagacaa ccgcttcgaa ggcgtgcacg
     1681 aaagcgcgca gtcgggcgag cgcgcggcgc gactgtcgcg atcgctctcg gcggtgctga
     1741 cgggagtgct cgagggcgga cagcgcgtcg accggcgcgg cggcgagggc atcccggggg
     1801 gcaccgggag cagctacgac gtcgaggact ggttcggtct ggtgggcgcg ggcatcgtgc
     1861 tcgcgccggg ggcgaacctc gatgtcgacg tgcgcgccgc cacgctcagc gacgccgagt
     1921 cgggcgccga gcagcgctcg ctcaaggtga cgccgcgtct ggtgtggcgc atcgccgacc
     1981 aactgaatct cttcggcacc tacgagcttg cgcaagtgac cgaccagaac cagaccggcg
     2041 tccggcccct ggtgttcgcg cgcgacggca cctcgcagcg ctggagcatc acgcccaaca
     2101 ttcgcatctc gaacatcatc ggcatcttcg cgacctactc ggggcgcaat gagcgcgtgt
     2161 tcacggggca gcgcgtggtc gagcacgagt tccgcctgga gacgcgggcg tacttctaat
     2221 gacgcgtcgt accggcatca tcgtcacggt cttggcgctg ttcgcggcgc tgccgccgat
     2281 tgcgttggcg gccgacggtg cgccggcgcg cacgacgaaa ctgcgcgtgc tgggtacgca
     2341 cgcgctgccc ggcgagcgcg ccgaagccct cctgcgcgag aacggttttc ccgccgcgcg
     2401 cgcgctgcgc atcctgcaag aggcgtacat cggcgaaggc tatctggccg cgtcgatcgc
     2461 ggtgacggtg gaggccgact cgtcgttcac ggtggtgatc gacgagggcc agcggccgcg
     2521 cgtcgggagc gcgcgcgcga acggcctgcg ggcgcgtacc gaaggcgacg tcctggcggc
     2581 actggagctg accggcggtt cgcccttcga gcccaagcgg ctcgcgcgca acatcgacgc
     2641 cctgctggcc tcgtacgaca acgagggcta tcccttcgcg caggtctggc tggacagcct
     2701 ggggctctcc gccgacgcga ccaccgtcgc cgtctcgctg accgtggtcg agggcgaacc
     2761 gcgcgcgatc gaaggcgtgg tggtggaggg cttgcagaag acgcggcccg cgctcgcgca
     2821 acgcatcgcg gcgctcgaaa ccggggtgcc gtaccgcgcg caggcgctcg aagacgccta
     2881 cctgcgcatg gtggcgagcg gcgtgttcac cgacgtcgag ttcccgaccg tgcgactgtc
     2941 gtccgacggc cgcggtgtgg acgccgttat cacggtggta gagccgaagc gctcgcacac
     3001 gttcgcggcg gcgctgggct acgcgtccag cgacggcacg cgcgaccgcg tgctctcggg
     3061 gctcgtgcag ctcgatctca acaatgtggg cggaaccctc aaggactttg gggcgtcgtg
     3121 gaacaacgac ggcgtggggc gcagcgagac gcgcatcgcg taccgcgacc ggttgttcct
     3181 cgggcggcgg ctggcggtgg gtctccaact cgagcaggtc gggcaggaca ccgtgtacac
     3241 gtggcagtcg gccgatctcg acgtcgaacg cggagtcggg cgcgtggcgg ggatgctgac
     3301 cagcgtatcc gtgggcttcg cgggagaccg caacgtgtac agcgtcggcg acctggtgaa
     3361 ctcgacgcgg ctgcgcgcgc gcgcgggcgc gagcgtgctc gcgggaagcg agcggcgcgc
     3421 ggccttcgcc cgcgtgggag tttcggccac gctggcccag aaggacctgt cgtaccgaga
     3481 gggcgcgacc ggccccggcg atgtctcgca gtggatctac gacggacgat tcgatctgct
     3541 ggtgccggcg ttctggtcgt tgtactacag cctggagggt cgcgcccaga cgctcgaaag
     3601 tgacgaagca accgtcccct tgccggagca attctacctg ggcggcgccc gcacggtgcg
     3661 cggctaccgc gagaaccagt tccacgggcg tcgcgtcgcg ttcg
//
LOCUS       DASPGK010000030        16931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1412891, whole genome shotgun sequence.
ACCESSION   DASPGK010000030 DASPGK010000000
VERSION     DASPGK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412891"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..6491
                     /locus_tag="VEC56_00760"
     CDS             21..6491
                     /locus_tag="VEC56_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXV12710.1"
                     /translation="MLLALDGVAPAPVTDLAISTTTSNTVFVAWTASGDDGVAGTAAR
                     YDLRYSTAPIDAGNFEAATPVAGIPAPRPSGTPESVEVTGLDFDTTYYFALVVEDELT
                     NRSPVSNPASGTTLGAPGLAFAPDTFSADVLTGGRSTHSLTIKNTAQGTLDFFFDDAP
                     SWLRTDPLSGRVYAGESLVVDVVFDATRVFGGEYRETVVLHSNDPVGPESPIELVLRV
                     TDAPDIAAVPSEIDFGVRYTGTCATDTVLVSNIGSEALTVDAVAVNNPEFTVDTGGFV
                     LGVGESRPLAVTFCPVVFEAGGPIRFPRRSRGVLSMTSNDPDHPRYAVPLFGEGIEPP
                     VIAVAPASFDEELFTGASATHTLTIGNDGATELDFEIVLEETGGVRVEVIADPSLDAG
                     VKAVGRPLSPGELAALTASVPKRVDMRADEHLDRDARAVTRTSGARMLERISAGNLEE
                     VFGSDQNEFLGGPRTRGNLFTCTTATTLREHRFYMNPTTATQVWFVVYEGATQDGVYD
                     LVSASDVTPAGPGLGWYSSGEIAVPLRAGKFYLIATAFEDAASYYNEQGIAPYPITTS
                     FGELTAGAGWSWEPYDQFPPAPFQFVTTGAFGEPVAYYQTLVTGGAVRWVSVDEAAQT
                     LPPGISIDVPLRFDASGMQGGEYAADVRVTSNDPVNPEVVVPVNLRVVKAPDIAISRT
                     RIDFGSAFVGTTAYDTLFVSNPGTDFLFVSATASSRPEYAVDAPVFVLGPGTRKLIVV
                     SFAPTTAGSLPATLSLTTNDPDQALVQIALAGEAREAPVLAIEPPSFLVDLASGEAAS
                     PVLVLTNSGGGELTYEIDTGLRPRPALSKRPPFPRRAADFPRGSHPASIGPAPRAGRS
                     DPPAASVPATSTAVAFSTETQNRRAARLRLDSPETLHLVGNAPDFIWAGDFGVGDNSF
                     AYAVNELNQFMTIDTLTGTQALLGSLVPFGTEVWTGMALDPTTGTLYAASTNVQQSSL
                     YVVDVAVPRATRVGAIGFPGIISLAVDDDGRMFAQDVITDELVSVEKSTGVGTAIGSL
                     GFDSNFGQGMAFDPVGEELYISAFNNFRFQSELRVADRTTGATSVVGVIGALEPGGLA
                     QLGWLGIPGLGGVPWLRASPRRGVVPPGQSVEVAVAFDAASLNGGDYDASLRITTDDP
                     ATPEITVLARLHVTGVPDIAASDSLLDYGVVFIGGRAKKTLVVSNVGTDVLNVSGVSV
                     TGDFAVDGSAFALAPGEDRELVVSLAPASAGSMLATLVIASNDADEGSFDVALRGEGR
                     EPPVMSIGPASFAENLFTGQSVTRAMTIDNSAGAADLIWNASTRYPGDARTVVATSPV
                     HRSRPIPDPLKEAGLPPARRPDPGGVYSFAPVALARAHPVDATSLESILASLDTLAGD
                     VTATIPDRFDFFDGAVSDGILDGGYDMYDGGNYLATNFGGFIPYSDGVIVDSPRFGAG
                     GRYFTRKYPGLFVLVAEMDNVEYFEIDGNLGADGGGSVDGAVLHARTAGADFLGFVKR
                     VYGAGDPSVNHMIIVQENEGASHEFSTDTNRDYHRAFGLSGSRRLYYLLYAGWDGAYI
                     DNTAALTVMTTFLNSLGLSPQWVRVTPAAGAVPAGESRVVDVVFDAARLVGDYVADIV
                     VAGNDPVTPEVAVPTGVHVDSAPDVALSTHLLRFDPIFVGAASQERFTISNAGSEVLT
                     VSAIASSAADFAADVSSTVLAPGESDGVAVTFAPLATGLRYASLSITSDDPDEATVVV
                     VLVGEGVEAPVVAVEPVSLADTLVAGDSSTHTLTVSNQGGTALDLRVTVEGGRAGTPP
                     ATPMIVTGGGPDYFGYRWQDTHDAGGPAFEWIDASGGTDVALADDGFVEGIPLGFSFR
                     YYGRTYTEIGVGANGWLSFNGSFPAFPASVPLADFCAGAIAPYARDLAPTMGAYVRHA
                     TIGTAPDRRFVIEYNQVPDAGGENPKTFQAILDERTGAIRFQYLAAPNAPEGLGIESP
                     DESVGLGDGGAGDTFIDPARVVDGYAIEFIAPPTWLTIAPTEASLPSGAGADFVVTMD
                     AAGLAGGEYHARLSIRSNDPVTPVVSIPVGLVVGAPLSVAGTGARGVPTRFALHPNRP
                     NPFNPVTTIAYDLPRASRVRLAIYDVRGREVRALIDANQPSGSHEVRWDGRDARGVPA
                     ASGVYFYRLSAGDFVQTKKMVLLK"
     gene            6514..7962
                     /locus_tag="VEC56_00765"
     CDS             6514..7962
                     /locus_tag="VEC56_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV12711.1"
                     /translation="MEIKHTKLLVNGEWIDSSDRSTTTLVNPATEEKLCDVATATAKD
                     VESAVASSRAAFDSGPWSKLDGGARAKLMWKLADLVERDGAEIARLETMNQGKPIFES
                     LKIEVPFVASLFRYYAGWADKLEGETIPVIPGFLNYTLREPVGVVGMIIPWNFPLLLT
                     VWKLAPALAAGCTALIKPSELTPLSALKLGALCLEAGFAPGVVNVITGKGSVAGQALL
                     EARGVDKIAFTGSTEVGRTVMKSAAGTIKRISLELGGKSPNIVFADAKLEAAANGACS
                     GIFYNKGEVCAAGSRLFVEKKVHDEFVEMIKTKMARYTPGDPLDDKTRLGPQVSKAHR
                     DSILKRVEEGAREGAKVAIGGRATAVNGKGYYVEPTVLTGVTNAMTVAREEIFGPVLC
                     VIPFEDENEAVKLANDSCYGLASGVWTQDPARAHRVAAALKAGTVWVNTYNVYHPASP
                     FGGYKESGFGRELGAQALDMYLEKKSVWVSLR"
     gene            complement(7987..10290)
                     /locus_tag="VEC56_00770"
     CDS             complement(7987..10290)
                     /locus_tag="VEC56_00770"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HXV12712.1"
                     /translation="MAICIAVLATAPARASDAASTPTVDASSAAYQPNTILTFDVPKL
                     AQAIRIDGRLDEPMWQTATKLTGFVEIDPGDNCRPKADTEAYLAYDAENLYIGFLCHD
                     DNPSAIRANITDRDAMFGDDFAGVMIDTFRDQQNGYEFFVNPRGIQGDLRRNRNNEDS
                     SYDAVWYSGGQVTSGGWSAEMAIPFRSLRFPDHDVQSWGIHVFRNRPRESREQMSWAP
                     ISRDNDCFFCQAGTMSGIAGVNQGRNLEVLPYALASQSSALTGSDDASFSWNDDDAAG
                     EAGVGLKYGVTPNHTLDFTYNPDFSQIESDATQIDANRTFALFYPEKRPFFLEGADMF
                     DSNMDIVYTRSINDPELAGKFTGKSGRNTVAFMSAWDETSPYIVPFEEQSLAAAGAGT
                     YSNILRVKRDVLTESYVGLMMTDRREADGDGSNTTFGADGLIRFNDNFRVNAQVTGSY
                     THEPDDTLMSANFPEIDFGDEDQYDSFFNGEEFAGFGSEVDVVRSGRHYNATLWYDDY
                     SPTFRAENGFVAQNDYRLLGFWNGYQFQLENNRLFERIEPQVEAARKYNYDGEFKDTW
                     LMPSLWLRFKKQTYFWAGYLWSEEVFAGTRVNGIERLNFDLDTAFSKYLSAGMYGHLG
                     HSVVRDRDNPRLGDEWSYGVYFNTKPLSQLRFDVTYDSFRLDELDGGPRIFDTFVSRA
                     KLTYQFSSKLFLRVIGEYVDDTQSYALDPLLSYKINPFTVFFLGSSHSFGNFEDDPAT
                     APIEVAQPGYKQTERLFFVKFQYLFRV"
     gene            10546..11253
                     /locus_tag="VEC56_00775"
     CDS             10546..11253
                     /locus_tag="VEC56_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXV12713.1"
                     /translation="MTKDALIRGSLSQSMENYLKAIFEILEHDQRATTSSIADRMGIA
                     PPSVTAMVKKLAELKLVSHEPYQGVTLTRVGEMAAIEVVRHHRLIEKYLADALGVPWD
                     LVHDEAERLEHVISEDLEDRIDGALGHPTVDPHGAPIPARDGTVVRVVARPLADASGG
                     DQLVVVEVDDRDPSLLRYLGERRLYPGTTVEVLRVEPYGGSTVLRIEGAEVAIGRDAA
                     AMIRVAPKIASGEEARP"
     gene            11250..12332
                     /locus_tag="VEC56_00780"
     CDS             11250..12332
                     /locus_tag="VEC56_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXV12714.1"
                     /translation="MTPAANLAVFFLGAALLGVVFWPRRGLFARGRQGRSLARRVELE
                     DALKHVYNHDARGQTSTLESLAGALEISSTSAVALVDRMQSVGLARVADGRILLTDEG
                     RRYALEVIRAHRLWERYLADETGIDPLEWHERAERKEHKLSREEADALSARLGDPRFD
                     PHGDPIPTADGAVFDGEVLPLTSLAQGERAMVAHVEDEPRVVYAQLVALGVYPGLVVC
                     VASKSEQRIEIESEGRRFVLAPLVAQNVSIRRIEIEEPAGAPVETLASLRPGEAADVV
                     RLSPACRGIERRRLMDLGIVPGTRVQLERRGLTGGLSAYHVRGTVIGLREEQASLIGV
                     VRREPAAEEKRGESAIRAGVLDEDMQ"
     gene            12329..14584
                     /gene="feoB"
                     /locus_tag="VEC56_00785"
     CDS             12329..14584
                     /gene="feoB"
                     /locus_tag="VEC56_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HXV12715.1"
                     /translation="MSTHTENSPSACAGCQHHIGNLVKLGLDMSKFDYVVALAGNPNT
                     GKSTVFNSITGLRQHTGNWPGKTVARAEGGFALDGRRYKLVDLPGTYSLLSTTVDEEI
                     ARNFILFARPDVTVVVVDASHLERNLNLVLQVLSITDRAVVCLNLIDEAERHGRRIDH
                     RRLARDLGVPVVPTVARSRRGIDDLLTTVAQVASGEIVCKPYRLARRSRQAERVVTEL
                     ADSVEATYPGLSNARWVALRLLDGDAHLEDALRSGELMELTAPAPGVAPAAGARAGSE
                     VLASEAAVARVIETARALRWKIGSEFHDSLTEDLYTEAARIADRATMVGEASGFDLDR
                     AIDNLVTHRVLGFPIMLAILTVVFWLTIAGANVPSGLIADFLIGRVHPWLHSLSGAIH
                     MPAWLAGFLFDGVYLATAWVVSVMLPPMAIFFPLFTLLEDFGYLARVSFNLDGLFKRA
                     GAHGKQALSMSMGFGCNAAGVVATRIIDSPRERLIAIITNNFALCNGRWPTQILIATI
                     FVGGLVPAHLSGLVSAGAVVTVAVLGVAMTFVVSWLLSRTVLKGEPSSFSLELPPYRP
                     PRFWHTLYTSIIDRTLIVLWRAVVFAAPAGAVIWLTGNIHVGGTSVAEHLVQAFDPFG
                     MLIGLNGVILLAYIVAIPANEIIIPTVLMLTVMTAGIAGVGSGSGVMFELDSIADVSQ
                     LLRAGGWTVLTGVNLMLFSLLHNPCSTTIYTIYKETRSRRWTTVAALLPVVMGLVVCF
                     LVAQVWRIVAP"
     gene            14643..15977
                     /locus_tag="VEC56_00790"
     CDS             14643..15977
                     /locus_tag="VEC56_00790"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXV12716.1"
                     /translation="MASRLTPEQLVTRTLGERSIPSPLTLSTHVGDGLAHFVPDRTRV
                     LYKVAGEKDADFDGHLFLERAGPRETLYFDPTKTRVGVVTCGGLSPGVNNVIRSMVLE
                     MTFQYGVRDIVGFRFGYRGMNPARGYDPVELNYDLVRTIHNEGGSVLGTSRGAEDVPV
                     MVDYIQDLGIDILFCIGGDGTLRGAHAIWEEVERRGAKIAIVGIPKTIDNDIPFVYKT
                     FGFDTAVEVVRQAVDGAHVEAIGTVNGVGLVKVMGRDSGFIAAYGSLASMQVNFCLVP
                     EVPFDLHGEGGFLDCLERRLMKHGHAVIVVAEGAGQQFFPDRSREMDASGNIVPRDIG
                     IFLREEVANYLRKRGHMFGMKYIDPSYMIRSVKANAGDAIFCDNLGRNAVHAGLAGKT
                     DVVIGLWHGIYTHVPIPLTVRGRKKIHPESYLWRSVVAATGQPMVMRARANR"
     gene            16094..>16931
                     /locus_tag="VEC56_00795"
     CDS             16094..>16931
                     /locus_tag="VEC56_00795"
                     /inference="COORDINATES: protein motif:HMM:NF042644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6588 family protein"
                     /protein_id="HXV12717.1"
                     /translation="MHPHSRTRGALWALIAFVVVAVPAVEAAADDGSVQNQVSVYTGD
                     NAVGYLQPLANALGAALNSSFGYSAYIPKTSFHISLEAPVMGLFFADDDKSFQASAES
                     GFVPLPGETSWSVPTVVGEGSAVVVDGTGGAQFAFPGGLQLNSFGLAVPQLRISSLAG
                     TEAVIRWVAYDQGDADVGKIDMFGIGGRHSISQYLGETPALDLTVGVLYQTLEVGEND
                     FGNPFVDSSALTVQGQASKRLPVGFATLEPYGIVAYESLDLDVEYADTNDQPVAVSME
                     AEN"
BASE COUNT         2692 a         5609 c         5715 g         2915 t
ORIGIN      
        1 ggcgactcaa catcaatact atgctgctcg cactcgacgg cgtggcgccc gccccggtaa
       61 ccgacctcgc gatatcgacg accacgtcca acacggtgtt cgtggcgtgg acggcgtcgg
      121 gcgacgacgg tgtggccggc acggccgcgc ggtacgacct ccgctattcc accgcaccca
      181 tcgacgcggg aaatttcgaa gcggccacac ccgtcgccgg gattccggcg ccgcgaccca
      241 gcggaacgcc cgaatcggtc gaggtgaccg gactggactt cgacacgacg tactacttcg
      301 cgctcgtcgt cgaagacgag ctgacgaacc gatcgcccgt ttccaacccg gccagcggaa
      361 ccaccctcgg cgcgcccggc ctcgcgttcg cgcccgacac cttcagcgcc gacgtgctga
      421 cgggcgggcg gtcgactcat tcgctcacga tcaagaacac cgcgcagggc acgctggact
      481 tcttcttcga tgatgcgccg tcgtggcttc gcaccgatcc cttgtccggg cgcgtgtacg
      541 cgggagagag tctggtcgtg gatgtggtgt tcgatgccac gcgcgtgttc gggggcgagt
      601 atcgcgaaac cgtggtcctg cacagcaacg atcccgttgg gccggaatcg cccatcgaac
      661 tcgttctgcg cgtcaccgac gcgcccgaca tcgccgcggt tccctccgag atcgatttcg
      721 gggttcgcta caccggcacg tgcgcgaccg acaccgtgct cgtctcgaac atcggctccg
      781 aagccctgac ggtcgacgcg gtcgccgtca acaaccccga gttcacggtc gacacgggcg
      841 gtttcgtgct cggggtggga gagagccggc cgctggcggt gaccttctgt cccgtggttt
      901 tcgaagcggg cggccccatt cgttttcccc gtcgcagccg gggcgtgttg tcgatgacga
      961 gcaacgaccc cgatcaccca cggtacgcgg tgccgctctt cggcgaggga atcgagccgc
     1021 cggtcatcgc ggtcgcgccg gcctcgttcg acgaggagtt gttcacgggc gcgagcgcga
     1081 cccacacgct gaccatcggc aatgatggcg ccaccgagct cgatttcgag atcgtcctgg
     1141 aagagaccgg cggcgtgcgc gtcgaggtga tcgcggaccc gtcgctcgat gcgggtgtga
     1201 aggcggtcgg gcgccccctg tcgcccgggg agctggccgc gctcaccgca tcggtcccca
     1261 agcgcgtgga catgcgcgcg gacgagcact tggaccgcga tgcgcgtgcg gtcacgcgca
     1321 cgagcggcgc gcggatgctc gagcgaatct cggccggaaa tctggaagaa gtgttcggga
     1381 gcgaccagaa cgagttcctg ggcggtccca ggacgcgcgg caatttgttc acgtgcacca
     1441 ccgcgaccac gctccgcgag caccgcttct atatgaaccc gaccacggcc acgcaggtgt
     1501 ggttcgtcgt ctacgaaggc gcgacgcagg acggcgtgta cgacttggtc agcgcctccg
     1561 acgtgacccc ggccggtccc ggcctgggct ggtacagctc gggcgagatc gcggtgccgc
     1621 tgcgcgcggg caagttctac ttgatcgcga ccgcgttcga ggacgcggcc agctactaca
     1681 acgagcaggg catcgcgccg tatccaatca cgacctcgtt cggagaattg accgcgggcg
     1741 cgggctggtc gtgggaaccg tacgaccagt ttcctcccgc gcccttccag ttcgtgacga
     1801 cgggcgcgtt cggcgagccg gtcgcgtact accagacgct ggtcaccggc ggcgcggtcc
     1861 gctgggtctc cgtggacgag gcggcgcaga cgcttccgcc cggcatctcg atcgatgtgc
     1921 cgcttcgctt cgatgcctcc gggatgcaag gcggcgagta cgcggccgac gtgcgcgtca
     1981 cgagcaacga tcccgtgaac cccgaggtgg tggtcccggt caatctgcgc gtcgtcaagg
     2041 cccccgacat cgcgatctcg cggacccgga tcgacttcgg tagcgcgttc gtgggcacga
     2101 ccgcgtacga cacactgttc gtttccaatc ccggcaccga ctttctattc gtgagcgcga
     2161 ccgcatcgag ccgtcccgag tacgcggtgg atgctccggt cttcgtgctg ggcccgggca
     2221 cgcgcaagct gatcgtggtg tcgttcgcgc ccaccacggc ggggtcgctc cccgcgacgc
     2281 tgtccttgac caccaacgac cccgaccaag cgttggtgca gatcgcgctc gcgggcgaag
     2341 cgagggaagc gccggttctc gcgatcgaac ccccctcgtt cttggtggac ctcgccagcg
     2401 gagaagccgc gtcacccgta ctcgtcctca ccaatagcgg tgggggtgag ctcacctacg
     2461 agatcgatac cgggctgcgt ccgcggcccg cgctgtcgaa gcgcccaccg ttcccgcgcc
     2521 gcgcggcgga ctttccgcgc ggaagccacc ccgcgtcgat cgggcccgcg ccgcgagcgg
     2581 ggcggagcga tcctccggcc gcgagcgtcc ccgcgaccag caccgcggtc gccttttcga
     2641 ccgagaccca gaatcggcgc gcggccagac tgcgcctcga ttcacccgag acgttgcatc
     2701 tggtcggcaa cgcccccgat ttcatctggg ccggcgactt cggcgtgggc gacaactcgt
     2761 tcgcgtacgc ggtcaacgag ctcaatcaat tcatgaccat cgacacgctg acggggacgc
     2821 aggcgctgct cggctcgctg gtcccgttcg gcaccgaggt atggaccggg atggcgctcg
     2881 accccaccac cgggaccctg tacgcggcca gcaccaacgt ccagcaatcg tcgctgtacg
     2941 tggtcgacgt cgcggttccg cgtgcgacgc gcgtgggcgc gatcggattc cccggcatca
     3001 tctcgctcgc ggtcgacgac gacgggcgca tgttcgcgca agacgtcatc accgacgagc
     3061 tggtgtcggt cgagaagtcg acgggtgtgg gcactgcgat cgggtcgctc ggcttcgatt
     3121 cgaatttcgg gcaggggatg gcgttcgacc ccgtgggcga agagctgtat atctccgcgt
     3181 tcaacaattt tcgcttccag tccgagctgc gggtcgcgga ccgcacgacc ggcgccacct
     3241 ccgtggtcgg tgtgatcggc gcactcgagc ccggcggcct cgcgcagctg gggtggctgg
     3301 ggataccggg gctagggggc gtgccgtggc tgcgcgctag cccgcgccgc ggtgtggtgc
     3361 cgccgggtca atcggtcgag gtcgcggtcg ccttcgacgc cgcgtcgctc aacggcggcg
     3421 attacgacgc gagcctgcgc atcaccaccg acgacccggc cactcccgag atcaccgtgc
     3481 tcgcgcgcct gcacgtgacc ggcgtgcccg acatcgcggc atcggattct ctgctggact
     3541 acggcgtggt gttcatcggc ggccgcgcca agaagacgct ggtggtgtcg aacgtcggca
     3601 ccgacgtgct gaatgtgagc ggcgtctccg tgacgggcga tttcgcggtc gacgggagcg
     3661 cgtttgcatt ggcgcccgga gaagaccgcg agctcgtggt ctccctggcg ccggcgtcgg
     3721 cgggttccat gctcgccacg ctcgtgatag cgagcaacga cgccgacgag ggttccttcg
     3781 acgttgcgct gcggggcgaa gggcgcgagc cgccggtgat gtcgatcggt ccggcgtcgt
     3841 tcgcggagaa cctgttcacc gggcagtcgg tcacgcgcgc gatgacgatc gacaactcgg
     3901 ccggcgccgc cgacttgatc tggaatgcgt cgacccgcta tccgggtgac gcgcgcacgg
     3961 tcgtcgcgac gagcccggtt caccgttcgc gcccgatacc cgatccgctc aaggaggcgg
     4021 ggttgccacc cgcccgacgc cccgacccgg gcggggtcta ctcgttcgcg cccgtcgcct
     4081 tggcccgcgc gcatcccgtc gacgcgacca gcctcgagtc gatcctcgcc agcttggaca
     4141 cgctcgcggg agacgtcacc gcgaccatcc ccgatcgctt cgacttcttc gacggagcgg
     4201 tgagcgacgg catcctcgac ggcggctacg acatgtacga cggcgggaac tatctcgcga
     4261 ccaacttcgg cggcttcatt ccgtactcgg acggcgtgat cgtggacagc ccgcgcttcg
     4321 gcgccggcgg ccgctacttc acgcgcaagt atcccgggtt gttcgtgctg gtcgccgaga
     4381 tggacaacgt cgagtacttc gagatcgacg gaaacctggg cgcggacggg ggcgggagcg
     4441 tggacggtgc cgtcttgcac gcgcgcaccg cgggcgccga tttcctcggt ttcgtcaagc
     4501 gcgtgtacgg agcgggcgac ccctcggtga atcacatgat catcgtgcag gagaacgagg
     4561 gagcgagcca cgaattctcg accgacacca accgcgacta ccaccgcgcg ttcgggctct
     4621 cgggctcacg acgcttgtac tacctgctct acgcgggctg ggacggtgcc tacatcgaca
     4681 acacggccgc gctcaccgtg atgacgacgt tcctcaactc gctcggcctc tccccgcaat
     4741 gggtgcgggt cacgccggcc gcgggtgcgg tgccggcggg cgagagtcgc gtcgtcgacg
     4801 tcgttttcga cgccgcccgg ctcgtgggcg attacgtggc cgacatcgtc gtcgccggca
     4861 acgatcccgt cacgcccgaa gtcgcggtgc cgacgggcgt gcacgtcgat tccgcccccg
     4921 acgttgcgct gtcgacgcat ctcttgcgct tcgatcccat cttcgtggga gccgcatcgc
     4981 aagagcggtt cacgatctcc aacgccggca gcgaggtcct gaccgtctcg gcgatcgcgt
     5041 cgagcgccgc cgacttcgcg gcggacgtgt cgagcacggt cctggcgccg ggagagagcg
     5101 acggggtggc ggtgacgttc gccccgctcg cgaccggact gcggtacgcg tcgctctcga
     5161 tcacgagtga cgatcccgac gaagcgacgg tggtcgtggt gttggtcggc gagggcgtcg
     5221 aggccccggt ggttgcggta gagccggtgt cgctcgccga caccctcgtg gcgggggatt
     5281 cgtcgacgca caccctcacg gtgtcgaatc agggcggcac cgcgctcgat ctgcgcgtca
     5341 ccgtggaagg cgggcgtgcc ggaacgccgc ccgcgacgcc catgattgtg acgggcggcg
     5401 gtcccgatta cttcggttac cgatggcagg acacccacga cgcgggcgga cccgccttcg
     5461 agtggatcga cgcgagcggg ggaaccgacg tcgcgctcgc ggacgacggc ttcgtcgaag
     5521 gcatcccgct cgggttctcg ttccgctact acggccgcac gtacaccgag atcggcgtgg
     5581 gcgcgaacgg ttggctgagt ttcaatggat cgtttccggc cttccccgca tcggttccgc
     5641 tggccgactt ttgcgcgggc gcgatcgcgc cctacgcgcg cgacctcgcg cccacgatgg
     5701 gcgcgtacgt tcgtcacgcc accatcggaa ccgcacccga tcgccgtttc gttatcgagt
     5761 acaaccaggt tcccgacgcg gggggagaga accccaagac gttccaagcg attctcgacg
     5821 aacggacggg cgcgattcgc tttcagtacc tggccgcacc caacgcgccg gagggattgg
     5881 ggatcgagag cccggacgag tcggtggggc tgggcgacgg cggagcgggc gacacgttca
     5941 tcgatcccgc gcgcgtggtg gacggctacg cgatcgagtt catcgcgccg ccgacgtggt
     6001 tgaccattgc acccacggag gcatcgcttc cctcgggcgc cggcgccgac ttcgtcgtga
     6061 ccatggacgc cgccggcctc gcgggcggcg agtaccacgc acggctctcg atccgcagta
     6121 acgacccggt gactcccgtg gtttcgattc cggttggtct cgtcgtcggc gcgccgttgt
     6181 cggtggccgg gacgggcgcg cgcggggttc cgacccgctt cgcgctccac cccaaccggc
     6241 ccaacccgtt caaccccgtc accaccatcg cgtacgacct cccgcgcgcg agccgcgttc
     6301 gccttgcaat ctacgacgtg cgcgggcggg aagtgcgggc cctaatcgat gccaaccagc
     6361 cgtccgggag ccacgaggtg cgctgggacg gccgcgacgc gcgcggcgtg cccgcggcca
     6421 gcggcgtcta tttctaccgc ttgagcgcgg gcgacttcgt gcagaccaag aagatggttc
     6481 tcttgaagta gcggttgtct tagaataccg cccatggaaa tcaaacacac caagctgctc
     6541 gtgaacggcg agtggatcga ctcctcggac cgttccacca cgaccctcgt caacccggcc
     6601 accgaagaga aactgtgcga cgtcgccacc gccacggcca aagacgtcga gtccgcggtc
     6661 gcgtcgtcgc gcgccgcgtt cgactccggc ccctggagca agctcgacgg tggagcgcgt
     6721 gccaagctca tgtggaagct cgccgacttg gtggagcgcg acggggccga aatcgcgcgc
     6781 ctggagacca tgaaccaggg caagcccatc ttcgagtcgc tgaagatcga ggtgcccttc
     6841 gtcgcgagct tgtttcgtta ctacgcgggg tgggccgaca agctcgaggg cgaaaccatt
     6901 cccgtcatcc ccggattctt gaactacacg ctgcgcgagc cggtcggcgt ggtcggcatg
     6961 atcatcccgt ggaacttccc gctcttgttg acggtgtgga agctcgcgcc cgcgctggcc
     7021 gcggggtgca ccgcactcat caagccgtcc gagctgacac cgctctccgc gttgaagctg
     7081 ggcgcgttgt gtctcgaggc gggcttcgcg cccggagtcg tcaacgtgat caccggcaag
     7141 ggctcggtgg cggggcaggc cttgttggaa gcgcgcggtg tggacaagat cgccttcacc
     7201 ggctcgaccg aagtcgggcg caccgtgatg aaatcggcgg ccgggacgat caagcgtatt
     7261 tcattggagc tgggcgggaa gtcgcccaac atcgtgttcg ccgacgccaa actggaagcg
     7321 gcggccaacg gcgcctgcag cggcatcttc tacaacaagg gcgaggtgtg cgcggcgggt
     7381 tcgcgcctgt tcgtcgagaa gaaggtgcac gacgagttcg tcgagatgat caagaccaag
     7441 atggcgcgtt acaccccggg cgatccgctc gacgacaaga cgcgcctggg cccgcaggtg
     7501 tcgaaggcgc accgcgactc gattttgaaa cgcgtcgaag agggcgcgcg cgagggggcc
     7561 aaggtcgcga tcggaggtcg cgcgaccgcc gtcaacggca agggatacta cgtggagccg
     7621 acggtactga ccggggtcac caacgcgatg acggttgcgc gcgaggagat cttcggaccg
     7681 gtgctgtgcg tgatcccctt cgaagacgag aacgaagcgg tgaaactcgc caacgatagc
     7741 tgctacggtc tcgcgtcggg cgtgtggacg caggaccccg cgcgcgcgca tcgcgtcgcg
     7801 gccgcactca aagccggcac ggtgtgggtc aacacctaca acgtgtacca cccggcttcg
     7861 cccttcgggg gctacaaaga aagcggcttc ggccgcgagc tgggcgcgca ggcgctcgac
     7921 atgtacctcg agaagaagtc ggtgtgggtg agcctgcggt agcgtccccc gccgggcagc
     7981 gcctcgctac acccggaaca gatactggaa cttcacgaag aacaaccgct cggtctgctt
     8041 gtagcccggc tgcgcgactt caatgggtgc cgtggccggg tcgtcctcga agtttccgaa
     8101 cgagtgcgac gagcccagga agaacaccgt gaacgggttg atcttgtagc tcaagagcgg
     8161 gtcgagcgcg tatgactgcg tgtcgtcgac gtactctccg ataacccgca agaagagctt
     8221 gctcgagaac tgataggtca gcttcgcgcg gctgacgaag gtgtcgaaga tgcgcggtcc
     8281 gccgtccaat tcatccagcc gaaacgagtc gtaggtaacg tcgaagcgga gctgcgagag
     8341 cggcttggtg ttgaagtaca cgccgtagct ccactcgtcg cccaagcgcg ggttgtcgcg
     8401 gtcgcgtacc accgagtggc cgaggtggcc gtacatcccc gcggagaggt acttgctgaa
     8461 cgcggtgtcg aggtcgaagt tcaaacgctc gatcccgttg acccgcgtgc ctgcgaacac
     8521 ttcctcgctc cacaggtaac ccgcccagaa gtaggtctgc ttcttgaaac gcagccacag
     8581 cgacggcatc agccatgtgt ccttgaactc cccgtcgtag ttgtacttgc gcgccgcctc
     8641 gacctggggc tcgatgcgct cgaacaaacg gttgttctcg agctggaact ggtagccgtt
     8701 ccaaaagccc aatagtcgat agtcgttctg ggccacgaag ccgttctcgg cgcggaaggt
     8761 cggcgagtag tcgtcgtacc agagggtcgc gttgtagtgg cggccgctgc gcacgacgtc
     8821 gacctccgag ccgaagcccg cgaactcctc gccgttgaag aacgagtcgt actggtcttc
     8881 gtcgccgaag tcgatttccg ggaagttggc cgacatcaag gtgtcatcgg gctcgtgggt
     8941 gtaactgccg gtcacctgcg cgttgacgcg gaagttgtcg ttgaagcgga tcagcccgtc
     9001 cgcgccgaag gtggtgttgg agccgtcgcc gtcggcttcg cgccggtcgg tcatcatcag
     9061 gccgacatac gattccgtga ggacgtcgcg cttgacgcgc agaatgttcg agtacgtgcc
     9121 cgcgcccgcg gcggccaggc tctgctcttc gaagggaacg atgtagggcg acgtctcgtc
     9181 ccacgcgctc atgaaggcga ccgtgttgcg gccggatttg cccgtgaact tgcccgccag
     9241 ttcggggtcg ttgatcgagc gcgtatacac gatgtccatg ttgctgtcga acatgtcggc
     9301 gccctccagg aagaagggac gcttctccgg atagaagagc gcgaaggtac ggttggcgtc
     9361 gatctgggtg gcgtcggact cgatctggct gaaatccggg ttgtaggtaa agtcgagcgt
     9421 gtggttcggc gtcacgccgt acttcaagcc gacgccggcc tcgccggccg cgtcgtcgtc
     9481 gttccacgag aaggacgcat cgtcggaacc ggtcagcgca ctcgactgac tcgcgagcgc
     9541 gtagggaaga acctccaagt tgcgcccctg gttcacacct gcaatacccg acatggtccc
     9601 cgcttggcag aagaaacaat cgttgtcgcg cgagattggc gcccacgaca tttgctcgcg
     9661 gctctcgcgc gggcggttgc ggaagacgtg gatgccccac gactgcacgt cgtggtcggg
     9721 aaaacggagg ctgcggaacg ggatcgccat ctccgcgctc catccgccgc tggtcacctg
     9781 cccgcccgaa taccagacgg cgtcgtagct cgagtcttcg ttgttgcgat tgcggcgaag
     9841 gtcgccttgg attccgcgcg gattgacgaa gaactcgtag ccgttttgct gatcgcggaa
     9901 ggtgtcgatc atgaccccgg cgaagtcgtc gccgaacatg gcgtcgcggt cggtgatgtt
     9961 ggcgcgaatc gcggaggggt tgtcgtcgtg acacaagaac ccgatgtaga ggttctccgc
    10021 gtcatacgcg agataggcct cggtgtccgc cttggggcgg cagttgtcgc ccgggtcgat
    10081 ctcgacgaag ccggtcagct tggtcgcggt ttgccacatg ggctcgtcga ggcggccgtc
    10141 gatgcggatg gcctgggcga gcttggggac gtcgaaggtg agaatggtgt tgggctgata
    10201 ggcggccgac gacgcgtcga cggtgggggt cgacgccgcg tcgctcgctc gcgcgggtgc
    10261 cgtcgcgagc accgcgatgc aaatggccac ggggtatgcc tgaatcgcgt cgatgcgcgc
    10321 tcgttctccg cgagcgcggg ggcgtgccga cagcatggtt cctcctcgaa caaggtcgcc
    10381 gctcgtctcg ccgtcccgtg gcacgtgggg atccggggcg gcgcttggat acggttaaga
    10441 cgtggggaga ggcaaaatgg tttcgtcgtt gccaacggga cggccgccag aggcgaaatc
    10501 gtcccttgac gtggaataag tcttgcacta gaatctgttt tagtcatgac taaagacgct
    10561 ctcatacgcg gatcgctctc ccagtcgatg gagaactacc tcaaggcgat cttcgagata
    10621 ctcgagcacg accagcgcgc gaccacctcg tcgatcgccg atcgcatggg gatcgccccg
    10681 ccgtcggtca ctgccatggt gaagaagctc gccgagctca agctcgtgtc gcacgagccc
    10741 taccaggggg tcactctgac ccgggtgggg gagatggcgg ccatcgaggt cgtccgccac
    10801 catcgcctga tcgagaagta cctggccgac gcactcggtg tcccgtggga cctcgtccac
    10861 gacgaggccg agcggctcga gcacgtcatc tcggaggacc tagaagaccg gatcgacgga
    10921 gccctgggcc acccgaccgt ggatccgcac ggcgcgccga ttccggcgcg cgacggcacc
    10981 gtcgtgcggg tggttgcgcg cccgctcgcc gacgcatccg gcggtgacca actggtcgtg
    11041 gtggaagtcg acgaccgcga tccgagcctg ctgcgctacc tcggcgagcg ccgtctctat
    11101 cccggcacca cggtcgaagt gcttcgtgtc gagccctatg ggggctcgac cgtgttgcgc
    11161 atcgagggcg cggaggtcgc gatcggtcgc gatgccgccg ccatgattcg cgttgcaccc
    11221 aagatcgcgt cgggcgagga ggcgcggcca tgaccccggc cgccaatctc gcggtgttct
    11281 tcttgggcgc cgcactcctc ggcgtggtgt tttggccgcg ccgcggcctg ttcgcgcgcg
    11341 ggcgccaggg gcggtcgctg gcgcgtcggg tcgagttgga ggacgctctc aagcacgtct
    11401 acaaccacga cgcgcgcgga cagacctcga cgctcgagag tctggccggg gcgctggaga
    11461 tctcgtcgac gagtgccgtg gcgctggtcg atcgcatgca gagcgtcggg ctggcgcgcg
    11521 tcgccgacgg ccgcattctc ttgaccgacg agggccgccg ctacgcgctc gaagtgattc
    11581 gcgcgcaccg gctctgggaa cgctacctcg ccgacgagac cggcatcgac ccgctcgagt
    11641 ggcacgagcg cgcggagcgc aaggagcaca agctctcgcg cgaggaagcg gacgcactgt
    11701 ccgcgcggct gggggatccg cgcttcgacc cccacggcga tcccattccc accgccgacg
    11761 gtgccgtctt cgacggcgag gtgcttccgc tgacctcgct cgcccaaggc gagcgcgcga
    11821 tggtggcgca cgtcgaggac gagccgcgcg tcgtgtacgc gcagttggtc gcactcgggg
    11881 tatatccggg gctcgtggtg tgcgtcgcgt cgaagagcga gcagcgcatc gagatcgaga
    11941 gcgaaggacg tcgattcgta ttggcaccgc tggtggcgca gaacgtgtcg attcgacgca
    12001 tcgagatcga agagcccgcg ggcgcgccgg tcgagacctt ggcgtcgctg cggccgggag
    12061 aagccgccga cgtcgtgcgc ttgagccccg cgtgtcgcgg aatcgagcgg cggcgcctga
    12121 tggacctcgg catcgtgccc gggacgcgcg tgcagctgga acggcgcggg ttgaccggtg
    12181 gtctctccgc ctaccacgtg cgcggcaccg taatcggcct gcgcgaggaa caggcgagcc
    12241 tgatcggcgt cgtgcgccgc gagcccgcgg ccgaggaaaa gcgcggcgag agcgcgatcc
    12301 gcgccggagt tctggacgag gacatgcaat gagcacccat accgagaatt cgcccagcgc
    12361 ctgcgcgggt tgccagcatc acatcggcaa cctcgtcaag ctcggtctcg acatgtcgaa
    12421 gttcgactac gtcgtggccc tggccggcaa tcccaacacc ggcaagagca cggtgttcaa
    12481 ctcgatcacc gggcttcgcc agcacaccgg caactggccc ggcaagacgg tggcgcgcgc
    12541 cgagggcggc ttcgcgctcg acggccggcg ctacaagctg gtcgatctgc ccggcaccta
    12601 ttcgctcctc tcgacgaccg tggacgaaga gatcgcgcgc aacttcatcc tgttcgcgcg
    12661 cccggacgtc accgtggtgg tggtcgacgc gtcgcacctc gagcgcaact tgaacttggt
    12721 gctgcaggtg ctctcgatca ccgaccgcgc ggtggtgtgc ttgaacctga tcgacgaagc
    12781 cgagcgtcac gggcggcgca tcgaccatcg tcgtcttgca cgcgacctcg gcgttccggt
    12841 cgtacccacg gtagcgcgca gccgccgcgg catcgacgac ctgctcacga ccgtggctca
    12901 ggtcgcttcg ggcgagatcg tgtgcaagcc ctatcgcctc gcgcgccggt ctcgacaagc
    12961 ggagcgcgtc gtcacggaac tcgcggactc ggtcgaggcc acgtatccgg ggctctccaa
    13021 cgcgcgctgg gtggcgctac ggttgctcga cggcgatgcg cacctcgagg atgcgcttcg
    13081 ctcgggggag ttgatggagt tgaccgcacc cgccccggga gtcgcgcccg ccgcgggcgc
    13141 gcgcgcgggc tccgaagtgc tcgcctccga ggccgcggtg gcgcgcgtga tcgaaacggc
    13201 gcgcgcgctg cgctggaaga tcggctccga gttccacgac tccctcaccg aggacctcta
    13261 caccgaagcc gcgcgcatcg cggaccgcgc gaccatggtg ggcgaggcgt ccggcttcga
    13321 cctcgatcgc gccatcgaca acctggtgac gcaccgtgta ctcggcttcc cgatcatgct
    13381 ggccatcctc acggtagtct tctggctcac gatcgcgggc gccaacgtgc cgtcgggatt
    13441 gatcgccgac tttctgatcg gccgcgtgca tccgtggctg cattcgctct ccggcgctat
    13501 ccacatgccg gcctggctgg cgggcttcct gttcgacggc gtctacctcg cgaccgcgtg
    13561 ggtggtgtcg gtgatgctcc cgcccatggc gatcttcttc ccgctgttca cgctgctcga
    13621 ggacttcggt tacctcgcgc gcgtatcgtt caacctggac ggcttgttca agcgcgcggg
    13681 cgcgcacggc aagcaagcgc tctcgatgag catgggcttc gggtgcaatg cggccggcgt
    13741 ggttgccaca cgcatcatcg acagcccgcg cgagcgcctg atcgccatca tcacgaacaa
    13801 cttcgccctg tgcaacgggc gctggcccac acagatcctg atcgcgacca tcttcgtggg
    13861 cggcctggtg ccggcgcacc tgtcgggcct ggtttcggcg ggagcggtgg tgacggtggc
    13921 ggtgctcggc gtggcgatga cgttcgtcgt gtcgtggctc ttgtcgcgca cggtgctcaa
    13981 gggcgagccc tcgtcgttca gcctggagct gccgccgtac cgtccgccgc ggttctggca
    14041 cacgctctac acctccatca tcgaccggac cctgatcgtg ttgtggcgag cggtggtatt
    14101 cgcggccccg gcgggggcgg taatctggct caccggcaac atccacgtgg gagggacgag
    14161 cgtcgccgag cacctggttc aggccttcga tccgttcggg atgctcatcg gattgaacgg
    14221 ggtcatcctg ctcgcgtaca tcgtcgcgat cccggccaac gagatcatca tcccgaccgt
    14281 cctcatgctg accgtgatga cggcggggat cgcgggggtg ggttcgggtt ccggcgtcat
    14341 gttcgagctc gattccatcg ccgacgtttc gcagctgctg cgggcgggag gatggacggt
    14401 tctgaccggc gtcaacctga tgttgttcag cctgctccac aacccatgca gcaccaccat
    14461 ctacaccatc tacaaggaga cgcgttcgcg ccggtggacc acggtggccg cactgctccc
    14521 ggtcgtgatg gggttggtgg tgtgcttcct cgtggcacag gtgtggcgca tcgtcgctcc
    14581 ttagcgcggc gtgaattcgc gctggacgcc ccggccggtc ttcctctatt tatagcgaag
    14641 gcatggccag tcgactcacc ccggaacaac tcgtcactcg aacgctcggc gagcgatcga
    14701 tcccctcccc actgaccctg tcgacccacg tcggggacgg gctcgcgcac ttcgtgcccg
    14761 accggacgcg cgtgctatat aaggtcgccg gcgagaagga tgccgacttc gacgggcatc
    14821 tgttcctcga gcgcgcggga ccgcgcgaga ccctctactt cgaccccacc aagacgcgcg
    14881 tgggtgtggt cacctgcggc ggcctctcgc ccggcgtcaa taatgtcatc cgctccatgg
    14941 tgctggagat gaccttccag tacggcgtgc gcgacatcgt cgggtttcgc ttcggttatc
    15001 gggggatgaa tcccgcgcgc ggctacgacc cggtcgaact caactacgac ctcgttcgca
    15061 ccatccacaa cgaaggcggc tcggtgctgg gcacctcgcg cggcgccgag gacgtcccgg
    15121 tgatggtgga ctacatccag gatctcggga tcgacatcct gttctgcatc ggcggagacg
    15181 gaaccttgcg cggcgcacac gcgatttggg aagaggtcga gaggcggggc gcgaagatcg
    15241 cgatcgtcgg catccccaag accatcgaca acgacattcc gttcgtctac aagaccttcg
    15301 gtttcgatac cgcggtcgaa gtcgtgcgcc aggcggtcga cggcgcccac gtggaggcca
    15361 tcggcaccgt caacggcgtg gggctggtca aggtgatggg gcgcgactcc ggcttcatcg
    15421 cggcgtacgg gagcctcgcc agcatgcagg tgaacttctg cttggtgccc gaagtcccct
    15481 tcgacctgca cggcgagggc ggcttcctcg actgcctgga acggcgactg atgaaacacg
    15541 ggcacgccgt gatcgtggtc gccgagggcg ccggccagca gttcttcccg gaccgctcgc
    15601 gcgagatgga cgcgtccggc aacatcgtcc cgcgcgacat cggtatcttc ctgcgcgagg
    15661 aggtggccaa ctacctgcgc aagcgcgggc acatgttcgg catgaagtac atcgacccca
    15721 gctacatgat ccggagtgtc aaggccaacg ccggtgacgc catcttctgc gacaacctcg
    15781 gccgcaacgc ggtgcacgcg gggctggcgg ggaagacgga cgtggtgatc ggactgtggc
    15841 acggcatcta tacgcacgtt ccgatcccgc tcacggtgcg cgggcgcaag aagatccacc
    15901 ccgagagcta cctgtggcgc agcgtggtcg cggccacggg ccagcccatg gtgatgcggg
    15961 cgcgcgcgaa ccgctagccg cctcggggag gcccgtcccc gcggccgcgc cccgatttgc
    16021 aattttccct gtgccgggtg tattctccgc gtgtcgtacc atcgcatctt ccacctacga
    16081 aaggagactg gatatgcacc cgcactcccg aacccgcggc gcactctggg ctctgatcgc
    16141 gttcgtcgtc gttgcagtgc ccgcggtaga ggccgcggcc gacgacggct cggttcagaa
    16201 ccaggtttcc gtgtacacgg gtgacaacgc cgttgggtac ttgcagccgc tcgccaacgc
    16261 gctcggcgcc gcactcaaca gctcgttcgg ttactccgcg tatattccca agaccagctt
    16321 ccacatcagc ctcgaggccc ccgtcatggg cctgttcttc gcggacgacg acaagtcctt
    16381 ccaggcgtcc gccgagtccg gcttcgtccc gctcccgggc gagacgtcgt ggtcggtccc
    16441 gaccgtggtg ggcgaaggca gcgccgtggt cgtcgacggt accggcggcg cgcagttcgc
    16501 gttccccggc ggcttgcagc tgaactcgtt cggcttggcg gtcccgcagc tgcgaatctc
    16561 ttcgctcgcc ggcaccgagg ccgtgattcg ctgggtcgcg tacgatcaag gcgatgccga
    16621 cgtgggcaag atcgacatgt tcggcatcgg cggccgccac agcatctcgc agtacttggg
    16681 cgagactcca gcactcgacc tgaccgtcgg cgttctttat caaaccctcg aggtgggcga
    16741 gaacgacttc ggaaacccct tcgtcgattc gtccgcgctg accgtccagg gacaggccag
    16801 caagcgcctg ccggtcgggt tcgccacact ggagccgtac ggcatcgtgg cctacgagag
    16861 cctcgacctc gacgtcgagt acgcggacac caacgaccag ccggtcgcgg tctcaatgga
    16921 agccgaaaac t
//
LOCUS       DASPGK010000031         7781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1647986, whole genome shotgun sequence.
ACCESSION   DASPGK010000031 DASPGK010000000
VERSION     DASPGK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647986"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VEC56_00800"
     CDS             complement(<1..289)
                     /locus_tag="VEC56_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV12718.1"
                     /translation="MHTKRNLFEGIPGSLADEWTERLAGDAGARVERIVSRGHSSPPD
                     FWYDQAQSEWVMVVKGSARIRFEDGRVVELRAGDHVTIAPHERHRVDWTAPG"
     gene            complement(291..1361)
                     /locus_tag="VEC56_00805"
     CDS             complement(291..1361)
                     /locus_tag="VEC56_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12719.1"
                     /translation="MARTRRAAGYGMILALLVLAPIARAQGGAPASPLAQAEELLDKN
                     QPKQAVELAKKAVAQEPNNPSAWFVLARANHAAGDLNGAIAAGHKAAEFAPVRASAFY
                     NLACAYAVKGDKDDAFKALSGAKRAGFADRDQMASDPDLASLRTDPRFQLPLERNYFT
                     LKLTDAATLPFSVDLPVGYEPGRAYPVLIAPGHGKKVEGNWGGLFWGEDTSQRGWITV
                     ESPALLTADAVDVTAQLLDEVARRYQVEGGKFHLACYGPSSGPAFGVAMAHSLRIKSL
                     VAIPGFPTTEKPEELKTLGGIRVLFIVGEKDTIWRPQVEVAHARLQQLGVDSYLEVVP
                     GAGHLLQEMFGGELAERLQLLR"
     gene            complement(1361..1831)
                     /locus_tag="VEC56_00810"
     CDS             complement(1361..1831)
                     /locus_tag="VEC56_00810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXV12720.1"
                     /translation="MSEHRASIIWTKQTESFRYEDYNREHTWTFPKSGIVVQATAAPK
                     YRGKPESVDPEEALVAAISSCHMLTFLALCARKGVVVERYEDEAVGWLEPNEERRLAI
                     TRVVIKPSITFAAGHQPDAEALAKLHEEAHEHCFIANSVRTAIAVEGSAQEVAR"
     gene            complement(1872..2954)
                     /locus_tag="VEC56_00815"
     CDS             complement(1872..2954)
                     /locus_tag="VEC56_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXV12721.1"
                     /translation="MKWSIRIGALFGIPVYIHLTFLLLLVWIGVLSWREGESARAGLE
                     GVLFISTIFACVVLHELGHALTARRYGIVTRDITLLPIGGVARMERMPDDPRQELWVA
                     LAGPAVNVVIAAVLIAITGVAAAIPDFSLTSGTFLNRLIAVNVFLVLFNLLPAFPMDG
                     GRVLRALLATRMEYTRATHLAATVGQMMALLFGFVGLFVNPMLIFIAFFVWIGAAQEA
                     SMVQMKHSLAGIPVSRAMVTSFDTLAPNDTLEKAITATLAGTQKDFPVVEDGRVVGIL
                     LQGDLLAALARGGAHVPVKNVMQTDFEVMEASEMLDTAFRRLSARKSSTAPVSNQGRL
                     VGLVTMDNIGEFLAIAGALERTKRTA"
     gene            3035..3847
                     /gene="thiD"
                     /locus_tag="VEC56_00820"
     CDS             3035..3847
                     /gene="thiD"
                     /locus_tag="VEC56_00820"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXV12722.1"
                     /translation="MKQALTIAGSDSGGGAGIQADLKTFHAHGVFGTSVITAVTAQNT
                     KEVRHAFDLPPELVRAQLEAVLDDFDIAAAKTGMLSSAAIIDTVADVLEARRFEKLVV
                     DPVMISKSGFRLLRDDAIDALVRRLAPRALVVTPNLHEASLLAAMPIESLDDMKRAAE
                     KIGELGARAVVVKGGHARFALAVDVLWHADGTIEVLHPEGEVAERSVHGTGCTFSAAI
                     TARIARGEDVATAVRNAKRYITRVIRHAVEPGRGHAVGDAFYFLEGDDWERE"
     gene            complement(3978..4673)
                     /locus_tag="VEC56_00825"
     CDS             complement(3978..4673)
                     /locus_tag="VEC56_00825"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HXV12723.1"
                     /translation="MAKHIGIVACSAEGAALCYTTVCTEAPELMGKKHAHPEVSMHTH
                     PFSDYVRLMEAGRWDGVAALMLSSEAKLAKAGAELIICPDNTVHRVFDQVAKQAKLPW
                     LHIAEEVAREAGRQSFKKVGLIGTAWTMESSFYKEKLDDVGVDIVFPAEKDRKLVNRI
                     IFDELVAAQFTAESRAYFTGLISEMRGQGCEAVVLGCTEVPLIITEENSPLPVLDSTR
                     LLARAALRASLED"
     gene            complement(4703..5401)
                     /locus_tag="VEC56_00830"
     CDS             complement(4703..5401)
                     /locus_tag="VEC56_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011610234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HXV12724.1"
                     /translation="MHGADFLKRLDETIARRALLTHPFYQLWNEGALSLEALREYAKQ
                     YYAHVRVFPRYVSAAHSACDDIRVRQLLLENLVDEDEGEKNHPELWMRFADGLGVARD
                     AVRGAALLEKTRESVDTMTRLTRSEDFRDGLAALYAYESQVPMVAKTKREGLAKFYAL
                     DDARAVEFFTVHEEADIVHSAVERDLLVEHCRTEDEQTRAIAVADQAAGALWHFLDGV
                     YEAYCQGMCPGKTN"
     gene            5633..7456
                     /locus_tag="VEC56_00835"
     CDS             5633..7456
                     /locus_tag="VEC56_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXV12725.1"
                     /translation="MATTVHDTIPRQFLASVEGYHRPDAFRHKIAGSYRDISHGEVYA
                     RVSALACALRALGVGKRDRVALLSENRIEWVIADLATLSCGGVSVPLYATLPANQIEY
                     MLRDSEARIVFVSSSAQLAKIESIRGRLPELKHVIVFDADTTDDGALSLEAVVSDGRA
                     LAAEADFRSMVNDVSTSDWASIIYTSGTTGEPKGAILSHGNFMSNVRQSLEVFDIGPS
                     DVCLSFLPLSHVFERMAGYYTMMTAGVSIAYAESIETVPENLREVSPTIVCSVPRVYE
                     KMYARVLDSVAKSPPLRRALFWWAIGVGKAHLAASLAGGAGPFLSAQRRLARSLVFKK
                     LEARVGGKLRFFVSGGAPLAREIAEFFNGAGVRIMEGYGLTETSPVVALNTFENFRIG
                     TVGKPVPGVEVRIADDGEVLVRGDNVMQGYFKKPEATAEAMAGGWFHTGDIGHLDPDG
                     FLVITDRKKDLIATAGGKKVAPQPIEGRLKQHPLIAEAVLVGDRRPFITTLIVPNFER
                     LSHWAAEHGIPHDDRGRLVDAPAVRQLYQKIVDGVNADLAQYERAKGFTLLKDELTID
                     AGHLTPTLKVRRRIVEKTYRNEIEAMYQSGAAAHTATEVGG"
     gene            complement(7477..>7781)
                     /locus_tag="VEC56_00840"
     CDS             complement(7477..>7781)
                     /locus_tag="VEC56_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV12726.1"
                     /translation="WVFTVRALSFARGMAAPMPGVEQEDFVKVANFGRQSWPGLIAQW
                     RAVRVASTMLFESFDDEAWKRMGIASENPVSVRALAYIIAGHERHHVGVIRERYLG"
BASE COUNT         1287 a         2661 c         2547 g         1286 t
ORIGIN      
        1 cgccgggcgc cgtccagtcg acgcgatggc gctcgtgcgg tgcgatggtg acgtggtcgc
       61 cggcgcgcag ctcgaccacg cggccgtcct cgaaacgaat ccgcgcgctt cccttcacca
      121 ccatcaccca ctcgctctgc gcctggtcgt accagaaatc cgggggcgac gaatggccgc
      181 gcgaaacgat gcgctccacg cgcgcgcccg cgtcgcccgc caagcgctcg gtccactcgt
      241 cggcgagtga gccggggata ccttcgaaga gattgcgttt ggtgtgcacg ctaacgaagt
      301 agctggagac gctccgcgag ctcgccgccg aacatctcct gcaggagatg ccccgcgccc
      361 ggcacgacct cgaggtacga atcgaccccg agctgctgga gccgggcgtg tgccacttcc
      421 acctggggcc tccagatcgt gtccttctcg ccgacgatga acagcacccg aatcccgccc
      481 agcgtcttga gctcttccgg cttctcggtg gtcggaaagc ccgggatcgc gaccaggctc
      541 ttgatgcgca gcgagtgcgc catcgcgacc ccgaacgcgg gtcccgacga cgggccgtag
      601 cacgcgaggt ggaacttgcc gccttccact tggtagcgtc gcgcgacttc gtccaggagc
      661 tgcgcggtca cgtccaccgc gtcggcggtc aagagcgcgg ggctctcgac cgtgatccac
      721 ccgcgctgcg acgtgtcctc gccccagaac aacccgcccc agttgccttc caccttcttg
      781 ccgtggccgg gcgcgatcaa caccggatac gcgcgccccg gttcgtaccc gaccgggaga
      841 tcgaccgaga acggcagcgt ggccgcatcc gtgagcttca gcgtgaagta gttgcgctcg
      901 agcgggagtt ggaagcgcgg gtcggtgcgc agcgacgcca agtcggggtc gctcgccatc
      961 tggtcgcgat ccgcgaagcc ggcccgcttg gcacccgaga gcgccttgaa cgcgtcgtcc
     1021 ttgtcgccct tgaccgcata cgcgcaggcc agattgtaga acgcgctcgc gcgcacgggt
     1081 gcgaactcgg ccgccttgtg cccggccgca atggcgccgt tcaagtcgcc tgccgcgtgg
     1141 ttcgcgcgcg cgagcacgaa ccacgcgctg gggttgttcg gttcctgcgc gacggccttc
     1201 ttcgcgagct ccaccgcctg cttgggctgg ttcttgtcga gcagctcctc ggcctgcgcg
     1261 agcggcgacg cgggagcacc accctgcgct cgcgcgatgg gtgcgagcac gaggagtgcc
     1321 aggatcatcc cataccccgc ggctcgcctc gttcgcgcca tcagcgcgcc acctcctgcg
     1381 ccgaaccctc caccgcaatc gccgtgcgca ccgaattggc gatgaagcag tgctcgtgcg
     1441 cctcctcgtg gagcttcgcg agtgcctccg cgtcgggttg gtgcccggcg gcgaaggtaa
     1501 tgctgggctt gatcacgacg cgcgtgatgg cgaggcgccg ctcttcgttc ggctccaacc
     1561 atccgacggc ttcgtcttcg tagcgctcga ccacgacgcc cttccgcgcg cacagggcga
     1621 ggaaggtgag catgtggcag ctcgagatgg cggccaccaa ggcctcctcg gggtcgacgg
     1681 actccggctt gccgcggtac ttgggcgccg ccgtcgcctg gacgacgatg ccgctcttgg
     1741 gaaacgtcca cgtgtgctcg cggttgtagt cctcgtaacg aaacgactcc gtctgtttcg
     1801 tccagataat ggacgcacga tgttcggaca cgattgcttc ctttcttgcg gcccctggga
     1861 cggcggcgtt ttcatgccgt ccgtttggtt ctctccagcg cgccggcaat ggccaggaac
     1921 tcgccgatgt tgtccatcgt gaccaacccg accaggcggc cttgattcga taccggtgcg
     1981 gtgctcgatt tgcgagcgct caagcggcga aacgcggtgt cgagcatctc cgacgcctcc
     2041 atgacttcga agtcggtctg catcacgttc ttgaccggaa cgtgggcgcc gccgcgcgcg
     2101 agcgcggcca ggagatcccc ctgcaggagg attcccacca cgcgaccgtc ttcgaccacc
     2161 ggaaagtcct tctgggtgcc cgcaagtgtg gcggtgatcg ccttctccag cgtgtcattg
     2221 ggggctagcg tgtcgaagct cgtcaccatc gcgcggctga ccggaatgcc cgcgagcgag
     2281 tgcttcatct ggaccatgct cgcttcctgc gccgcaccga tccagacgaa gaacgcgatg
     2341 aagatcagca tgggattgac gaacaacccc acgaagccga acaagagcgc catcatttgt
     2401 cccaccgtag ccgccaggtg ggtggcgcgc gtgtactcca tgcgcgtggc gaggagtgcc
     2461 cgcagcacgc gcccgccgtc catgggaaag gcgggcagca ggttgaacag caccaggaag
     2521 acgttgacgg cgatgagccg gttcaagaac gtcccgctgg tcagactgaa atccgggatg
     2581 gcggccgcga cacccgtgat cgcgatcagc accgcggcga tcacgacgtt caccgccggg
     2641 ccggccagcg ccacccacag ctcctggcgg gggtcgtcgg gcatgcgctc catgcgcgcc
     2701 acaccgccga tggggagcag cgtgatgtcg cgcgtgacga tgccgtagcg gcgcgcggtc
     2761 agcgcgtgtc ccagctcgtg gagcacgacg cacgcgaaga tggtcgatat gaagagtacg
     2821 ccctcgaggc cggcgcgcgc actctcgccc tcgcgccagc tcaagacacc gatccacacc
     2881 aacagcagga ggaacgtcag gtgaatgtag accgggatgc cgaagagcgc accgattcga
     2941 atcgaccact tcatgccgtg aatatacacc ttctccaccc cgcggtcgaa cgctgcggtt
     3001 gtggtaaact ggacccgcga gcgcgtatta atcgatgaag caagcactca ccatcgcggg
     3061 ctccgactcc ggcggcggcg ccgggatcca agccgatctc aagaccttcc acgcccacgg
     3121 ggtgttcggc accagcgtga tcacggcggt cacggcccag aacacgaagg aagtgcggca
     3181 cgcgttcgac ttacccccgg agctggtgcg cgcgcagctc gaggcggtgc tggacgactt
     3241 cgacatcgcg gcggccaaga ccggcatgct ctcctcggcc gcgatcatcg acaccgtcgc
     3301 ggacgtcctc gaggcgcggc gcttcgagaa gctcgtcgtc gacccggtca tgatctcgaa
     3361 gagcggtttc cggctcctgc gcgacgacgc catcgatgcc ctggtgcggc gcctcgcgcc
     3421 gcgcgcgctg gtggtcacgc ccaacctcca cgaagcctcg ctgctggccg cgatgcccat
     3481 cgaatccttg gacgatatga agcgcgcggc ggagaagatc ggcgagctgg gcgcgcgcgc
     3541 cgtcgtggtc aagggcgggc atgcccgctt cgcgctggcg gtcgacgtac tgtggcacgc
     3601 ggatgggacg atcgaggttc tgcaccccga aggcgaggtc gcggagcgaa gcgtccacgg
     3661 caccggctgc accttttcgg ccgcgatcac cgcccgcatc gcgcgcggcg aagacgtcgc
     3721 aaccgcggtg cgcaacgcca agcgctacat cacccgcgtg atccgccacg ccgtcgagcc
     3781 cggccgcggc cacgcggtcg gcgacgcgtt ctacttcctg gaaggggacg actgggagcg
     3841 cgagtagtcc tttggagaca cggccgacgc gatcggtccc ccgacgcgat ttctgcggca
     3901 cgaatacgac acgatactgt agcttctcgt gccgcagttt gagcggagag ggacgatcgc
     3961 gtcggccgaa agagatctta gtcttcgagg gacgcgcgca aggccgcgcg cgccaagagg
     4021 cgggtcgaat cgaggaccgg gagcggcgag ttctcttcgg taataatgag cgggacctcg
     4081 gtgcagccca gcacgacggc ctcgcagccc tggccgcgca tctcggagat caagcccgtg
     4141 aagtaggccc gcgactccgc ggtgaactgc gccgccacca gttcgtcgaa gatgatccgg
     4201 ttgacgagtt tgcggtcctt ctcggccggg aacacgatgt cgacacccac gtcgtcgagc
     4261 ttctccttat aaaagctcga ctccatcgtc cacgcggtgc cgatcagtcc caccttcttg
     4321 aaactctggc gcccggcttc gcgcgcgact tcctcggcga tgtgcagcca ggggagcttg
     4381 gcctgcttgg cgacctggtc gaacacgcgg tggacggtgt tgtcggggca gatgatcaat
     4441 tcggcgccgg ccttggcgag cttcgcttcc gatgagagca tcagcgccgc cactccgtcc
     4501 cagcgacccg cctccatgag gcgaacgtag tcggagaacg ggtgggtgtg catgctcacc
     4561 tcggggtggg cgtgcttctt ccccatcaac tcgggggctt ccgtgcacac ggtggtgtag
     4621 cagagggccg cgccttccgc gctgcacgcg acgataccga tgtgcttggc caatgtgctc
     4681 tccgccggaa agagaattcg cgctagttcg tcttgcccgg gcacatgccc tggcagtagg
     4741 cctcgtacac gccgtcgagg aagtgccaca gcgcacccgc ggcctggtcg gcgaccgcga
     4801 tcgcgcgtgt ctgctcgtcc tcggtgcggc aatgctcgac caacaagtcg cgctccaccg
     4861 cgctatggac gatgtcggcc tcttcgtgga cggtgaagaa ctcgaccgcg cgcgcgtcgt
     4921 cgagcgcgta gaacttggcc agaccctcgc gcttggtctt ggcgaccatg ggaacctgcg
     4981 actcgtaggc atagagcgcc gccaggccgt cgcggaagtc ctcgctgcgc gtcaagcgcg
     5041 tcatcgtgtc gaccgactcg cgggttttct ccagcagagc cgcgccgcgc accgcgtcgc
     5101 gcgccacacc gagtccgtcg gcgaagcgca tccacagttc gggatggttc ttctcgccct
     5161 cgtcctcgtc gaccaggttc tcgagcagga gctggcgcac gcggatgtcg tcgcacgcgg
     5221 aatgggcggc gctgacgtag cgcgggaaca cgcgcacgtg cgcgtagtac tgcttcgcgt
     5281 actcgcgaag cgcctcgagc gagagcgcgc cctcgttcca aagctgatag aagggatgcg
     5341 tgagcagcgc gcgacgggca atcgtctcgt cgagccgctt caggaaatcg gcaccgtgca
     5401 ccttgggcac ctccgtacag tgggcaattc gtaatgcgga ctcccgccgg gcggcgggtc
     5461 cgtgggccag caattgcgct aatgtaaccg cgacgggccc ggatttcaaa ccttttgtgc
     5521 ggccgcgaaa agcgcgtggc ggcgccccgc gcgggcgctc gccgtgtcga ggttgacacc
     5581 cccgggtgcg ggccggatac tgggcacggc ccttgcacga gagccgctca ttatggccac
     5641 cacggtccac gacaccattc cccgccagtt cctggcctcg gtcgagggat accaccggcc
     5701 cgacgcgttt cgccacaaga tagccggaag ctaccgtgac atctcccacg gcgaggtgta
     5761 cgcgcgcgtg agcgccctgg cgtgcgcgct gcgcgcgctg ggagtcggca agcgcgaccg
     5821 cgtggcgctg ctgtccgaga accggatcga atgggtgatc gccgacctcg cgactctttc
     5881 gtgcggcggc gtcagcgtcc cgctctacgc gaccctgccc gccaaccaga tcgagtacat
     5941 gctgcgggac tcggaggcgc gcatcgtctt cgtgtcgtcg agcgcgcagc tggccaagat
     6001 cgaatccatt cgcggccgcc tccccgaatt gaagcacgtg atcgtcttcg atgccgacac
     6061 caccgacgac ggcgcgcttt cgctcgaggc ggtcgtttcc gacgggcgcg cgctggcggc
     6121 ggaagccgat tttcgttcca tggtgaacga cgtgagcacg tcggactggg ccagcatcat
     6181 ctacacgtcc gggaccacgg gcgagcccaa gggcgcgatc ctctcgcacg gcaatttcat
     6241 gagcaacgtg cgtcagtccc tcgaggtatt cgacatcggc ccctccgacg tgtgcctatc
     6301 gttcctgccc ttgagccacg tcttcgagcg catggcgggc tattacacga tgatgaccgc
     6361 gggggtatcc atcgcgtacg ccgagtcgat cgagaccgtg cccgagaatc tgcgcgaagt
     6421 ctctcccact atcgtgtgca gcgtgccgcg cgtgtacgag aagatgtacg cgcgcgtgct
     6481 ggacagcgtc gcgaagagcc ccccgcttcg tcgcgcgctc ttctggtggg cgatcggggt
     6541 ggggaaggcg cacctggccg cttcgctcgc gggcggcgcg ggcccgttcc tttccgccca
     6601 gcgccgcctc gcgcgctctc tcgtgttcaa gaagctggaa gcgcgcgtgg gtggaaagct
     6661 gcggttcttc gtttccggcg gcgccccgct ggcgcgcgag atcgccgagt tcttcaacgg
     6721 cgccggcgtc cgcatcatgg agggctacgg gctgaccgag acctcgcctg tggttgcgct
     6781 caatacgttc gagaactttc gcatcggcac cgtcggcaag ccggtgccgg gcgtcgaggt
     6841 ccggatcgcg gacgacggcg aagtgctggt gcgcggcgac aacgtgatgc agggctattt
     6901 caagaagccg gaggcgaccg cggaggcgat ggcgggcggg tggttccaca ccggcgacat
     6961 cggccacctc gaccccgacg gattcctcgt catcaccgac cgcaagaagg acctgatcgc
     7021 gaccgcgggc ggcaagaagg tggcgccgca gcccatcgaa ggacgactca agcagcaccc
     7081 gttgatcgcg gaagcggtgc tggtcggcga ccgccgaccg ttcatcacca ccctgatcgt
     7141 ccccaacttc gagcgccttt cccactgggc ggcggagcac gggatcccgc acgacgaccg
     7201 cgggcggcta gtcgacgctc ccgctgtgcg ccagctgtac cagaagatcg tcgacggcgt
     7261 gaacgcggac ctggcgcaat acgagcgcgc caagggcttc acccttctga aggacgagct
     7321 caccatcgat gccggccacc tgacgcccac gctcaaagta cgccggcgca tcgtcgagaa
     7381 gacctaccgc aacgagatcg aagcgatgta ccagtcgggc gcggcggccc acaccgccac
     7441 cgaagtcggc gggtagtcga ccggtcgcgg tcctccctag cccaggtagc gctcgcgaat
     7501 gacgcccacg tggtggcgtt cgtgtcccgc gatgatgtag gcgagcgcac gcaccgacac
     7561 cgggttttcc gacgcgatac ccatgcgctt ccacgcctcg tcgtcgaagc tctcgaacag
     7621 catcgtgctc gccacccgca ccgcgcgcca ctgcgcgatc aaccccggcc acgattggcg
     7681 accgaagttc gcgaccttga cgaagtcttc ctgttcgacg ccgggcatgg gggccgccat
     7741 tccgcgcgcg aaggacaacg cgcgcaccgt gaacacccac t
//
LOCUS       DASPGK010000032         8215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_8743, whole genome shotgun sequence.
ACCESSION   DASPGK010000032 DASPGK010000000
VERSION     DASPGK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8743"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="ccmA"
                     /locus_tag="VEC56_00845"
     CDS             <1..793
                     /gene="ccmA"
                     /locus_tag="VEC56_00845"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HXV12727.1"
                     /translation="RHRRRIPRSRRPRQYHLLIGMTASTPALACERLTKRYGHIAALK
                     SVDVAVDAGECVAVFGRNGAGKTTLLQVAGSLIRSYEGEVRVFGVSRRDAGPETRRAV
                     GLVLHEICLYDDLSALDNLRFFARLYGVTDHEARAHEVLARFDLADRANAVVRTLSRG
                     MKQRLAIARAVVHRPRLLLMDEPFTGLDEPASRALATALRDFVRDGGAVVMSTHDVER
                     AFDVATRAIILERGSITLDRSVADIDLAAFRHAYWHVLFTGTPRS"
     gene            805..1704
                     /locus_tag="VEC56_00850"
     CDS             805..1704
                     /locus_tag="VEC56_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12728.1"
                     /translation="MVLLPFLVVCFWQLLAVDSFSAEPIWLTDMRYCDLVVRGTIETS
                     SVELKKRNEVMPMLPPTESDVFVDVALVRMKVDEVMRGDFDAEHVAFVSFLSSSAFGD
                     ENYEPGRELVVGLIWGQNVLGGSYFLFSETTRFVRLGDAWEEQRGDTVLTDLSALRSA
                     LVKTTPRQILTQADAVVTGVVRDVRLDTVSDEGDRHAVQMTIALDNVRWLAGDGAATQ
                     LSVRQLMSGNYWPSWRDAAPWREMPIAGKEYCLFLKEIEEGYAVFGGVNGTFEVRDDH
                     LYFAGRTPVNLTVPEIRRALEEK"
     gene            1763..2425
                     /locus_tag="VEC56_00855"
     CDS             1763..2425
                     /locus_tag="VEC56_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXV12729.1"
                     /translation="MFRTASVVLWKDLRIEMRTRDAVSAALVFSILVLVIFNFALDVN
                     TPEMRRLAAGFFWVAFAFAGTLSLNRSFALEKESGAGRALSLAPVDPAGIFLGKALAN
                     AVFLAVTQIIVLPLFVVFFDVSVTRAWALAVSFALGSIAFAATGTLFSAIASNTRMRE
                     LLLPVLFLPVSVPALIAAVETTSFALGNTESASFWFTLLIVYDVVFVTASLLVFEYVV
                     EE"
     gene            2426..3130
                     /gene="ccsA"
                     /locus_tag="VEC56_00860"
     CDS             2426..3130
                     /gene="ccsA"
                     /locus_tag="VEC56_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXV12730.1"
                     /translation="MARRFGMVLDAALLVAMAAGFGMIFFYAPIESEMGIVQKIFYVH
                     VPLAWNAFLGFAIVFFASFRYLATRDPKWDARALAAAEVGVLFTTLVLITGPIWAKPV
                     WGIWWTWDARLTLTLVLWLIYVGYLMLRRYVDSPERRAVLSAVVGVTGFIDVPLVYFA
                     IRWWRTQHPQPVIAGGEGSGLDPRMASTLWVCVAALTLLFIALYRRRLAIEALRARAD
                     ALREEADAARAEGARS"
     gene            3127..3249
                     /locus_tag="VEC56_00865"
     CDS             3127..3249
                     /locus_tag="VEC56_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12731.1"
                     /translation="MSWLLTAYAVVAIAVGGYALRLARVKRALVSEIDAATRRR"
     gene            3345..4211
                     /locus_tag="VEC56_00870"
     CDS             3345..4211
                     /locus_tag="VEC56_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV12732.1"
                     /translation="MKPPKPSKSPANSPQRETRVQEDLALIEMVLGGSTAHWHAFVDR
                     YAGLIYSVIRRQLFAEEEDDVRTVFADVLEALYRGKLSEFRGSSELSTWLIVVSRGKA
                     LDHLRHIQGRRKNPQGWDGLTPLEKHVFKFHFVEGLGFDAVIQSLRSAGVRANAEAVA
                     HAVLKIEGTIDKHYLRRLESDAKAPALGVVSGRLLDYLKHMQFERERGEEDPDESFTR
                     EGIERMAARVRELLADLSDEERDVVRLRYEEGWTAQQIADERGLSGQRKVYTILDGAL
                     RKLRRLLDRDPE"
     gene            4307..5230
                     /locus_tag="VEC56_00875"
     CDS             4307..5230
                     /locus_tag="VEC56_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12733.1"
                     /translation="MTELHLTDEQLAALADDSMSESDRGLLLEHVRSCNACHDSYLDT
                     IRYRAILLADASVFRAPDEVVRLARSIARKGAAYDAVERHPRAEARRRWFAPPLLAGA
                     GVAVVALAAFAVWQSGVRIGGNRYDLYFSPLQQAAASASAEGSIVLPGTEDAAAATTP
                     LHRSGFVAADETISSALAQLTRAYQNDANPEVAQWLISGYLATGELEQARLFAEDARL
                     RFSDDARFLVLDAIVAYRSEEFDRAERLLQAALEAEPTNGAALLNLALVQYETGRADS
                     ARRTLELVRSQFPGSPLEARAEALISDLLNG"
     gene            5528..6559
                     /locus_tag="VEC56_00880"
     CDS             5528..6559
                     /locus_tag="VEC56_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXV12734.1"
                     /translation="MNTATHAHLLPLTLALISLVIPAESARPSEVALVIHGGAGTILR
                     DQMTPEREAALRAALTEALETGYRILQNGGSSLDAVEAAVCVLEDSPLFNAGRGAVFT
                     SEGKNEMDAAIMDGATRKAGAVASVKGIKNPVKAARSVMERTSHVMLVGEGAEKFARE
                     QGLQFEDSAYFFTQHRWDELKRTQEKEKELKKEYGSLAFPHTHLGTVGAVALDQNGNL
                     AAATSTGGLTNKRWGRVGDSPIIGAGTYADNATCAVSCTGRGEVFIRAAAAHEVSALM
                     RLEGFSVEKAAKRVVHEELVELGGEGTGGLIALDRKGRFAMECNTPGMYRGFVGADGK
                     ARTFIYRDE"
     gene            6571..7002
                     /locus_tag="VEC56_00885"
     CDS             6571..7002
                     /locus_tag="VEC56_00885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV12735.1"
                     /translation="MAILRLTLTSHTAEIKRIGGALSEFLDEEGIPPSLAHRARLVVE
                     ELVVNVIRHAFDDAREHAILLDVRTEPGGVAIVVEDDGKAFDPRDVRAPELRALVETG
                     KPGGLGLAMIKKLARDLTYARVDGRNRVRAFVGHSPRGGSS"
     gene            6999..7763
                     /locus_tag="VEC56_00890"
     CDS             6999..7763
                     /locus_tag="VEC56_00890"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HXV12736.1"
                     /translation="MTSRRRATALLLLLVLVAWWVACGDDDPVEPDNGAPAHRSEEIT
                     ASKDNTLYEEDGLCDACPGTNGVLDSLSNGAGEWVFAGTNNLGRARRALVAFDVAGNI
                     PAGAVVDSVVLTLTMSRTIAGTHTVALHRVLADWGEGTSDASCCAPEEGTGGTATVGD
                     ATWVLRVFRAAAWTTPGGDFAVASSSVRSVAGSGPYQWQSAQMTSDVQAWLDAPATNF
                     GWILIGDETVDRSAKRFDSRESAATAPRLTVYYTVP"
     gene            7817..>8215
                     /locus_tag="VEC56_00895"
     CDS             7817..>8215
                     /locus_tag="VEC56_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12737.1"
                     /translation="MRARSFLFGVAVVLHAPVSTHAQASPFDRLDVGLYGAANVNRND
                     FHSHWDAGYSGAIDFVTPFYLGRASLLVRVGTNDAIAGAATSGFTSVFGALGWRVGRS
                     IVDDLRTDLGLHVGLTEWIFSEEEQTEVRYE"
BASE COUNT         1334 a         2691 c         2733 g         1457 t
ORIGIN      
        1 ccggcatcgc cgaaggatcc cccgctcccg ccgcccgagg cagtaccacc tcctgatcgg
       61 aatgaccgcg tccacacccg cactcgcgtg cgagcgactc accaagcgct acggccacat
      121 cgccgcgctc aagtcggtcg acgtcgcggt ggacgcgggc gaatgcgtcg ccgtcttcgg
      181 ccgcaacggc gcgggcaaga cgacgctgct gcaagtcgcg ggctcgctga ttcgcagcta
      241 cgaaggcgag gtgcgcgtgt tcggagtgag ccggcgcgac gccggtcccg aaacgcgccg
      301 cgcggtcggg ctcgtgctcc acgagatctg tctctacgac gatctttccg ccctcgacaa
      361 tctgcgcttc ttcgcccggc tctacggagt aaccgaccac gaggcgcgcg cccacgaggt
      421 gctcgcgcgc ttcgacctcg ccgaccgcgc gaatgcggtc gtgcgcaccc tctcgcgcgg
      481 tatgaagcag cgcctcgcga tcgcgcgagc ggtcgtgcat cgcccaagac tattgctaat
      541 ggacgagccc ttcaccggcc tcgacgagcc cgcgagccgc gcgctggcca cggctctgcg
      601 cgacttcgtg cgcgacggcg gtgccgtggt catgagcacc cacgacgtcg agcgcgcctt
      661 cgacgtcgcg acgcgcgcga ttatcctgga gcgcggctcg atcaccctcg accgttcggt
      721 ggccgacatc gacctggccg cgtttcgcca cgcgtactgg cacgtactgt tcacggggac
      781 gccacggtcg taggagtgag gtcgttggtg ttgctgcctt ttcttgtcgt ctgtttctgg
      841 caattgctcg cagtcgactc attcagcgca gaaccgatct ggttgacgga catgcgatac
      901 tgtgacctcg tagtccgtgg cacgattgaa acctcgagcg tggagctaaa gaagagaaat
      961 gaagtcatgc ctatgctgcc gccaacagaa tcggatgtgt tcgttgatgt ggcactcgtg
     1021 cgcatgaagg tcgatgaagt aatgcgcggt gatttcgatg ccgagcatgt tgcttttgtt
     1081 tcgtttctga gttcctccgc gtttggggac gagaactacg aacccgggag agagctcgtt
     1141 gtcggtttga tttggggaca aaatgttctc ggcggaagct atttcctttt ttctgagacc
     1201 actcgattcg ttcgtttggg cgatgcctgg gaggaacaac gaggggatac agtgctcact
     1261 gacctttcag cgctgagaag cgccctggtg aagacgacac cgcgccaaat actcactcaa
     1321 gcagacgctg tcgtgacggg agtcgtccgc gacgttcgtc tagacacagt atccgacgag
     1381 ggcgatagac atgctgtgca aatgacgatt gcgctcgaca acgtgcggtg gctagctggc
     1441 gacggcgcgg ccacgcaact gagtgttcgg caactaatgt ctggaaacta ttggccgagc
     1501 tggcgcgacg cagctccatg gcgtgagatg ccgatagcag ggaaggagta ttgccttttc
     1561 ctgaaagaaa tcgaagaagg ttacgccgtg ttcggcggcg tcaacggaac ttttgaggtt
     1621 cgtgacgatc atttgtactt cgcgggtagg acgcccgtaa atctaacggt tcctgaaatt
     1681 cgccgagctt tggaggaaaa gtgatgcgga cctggacgcc gcgcaaccag cagacatctg
     1741 ctccccttat tgacgggtaa taatgttccg tacagccagc gtcgtcttgt ggaaggatct
     1801 ccgcatcgag atgcgcacgc gcgacgcggt cagcgcggcg ctggtgttca gcattctggt
     1861 gttggtcatt ttcaactttg cgctcgacgt caatacgccg gagatgcggc ggctggcggc
     1921 gggcttcttc tgggtggcgt tcgcattcgc ggggacgctg tctctgaatc ggtccttcgc
     1981 gctggagaag gagtcgggcg cggggcgcgc gctgtcgctg gcccccgtcg acccggccgg
     2041 catcttcctc ggcaaggcgc tcgccaacgc ggtctttctc gcggtcacgc agatcatcgt
     2101 gctgcccttg ttcgtggtct tcttcgacgt gtcggtgacg cgcgcctggg cgctggcggt
     2161 ctccttcgcg ctggggtcga tcgcgttcgc ggccacgggg acgcttttct ccgccatcgc
     2221 gtccaacacg cgcatgcgcg agctcctgct gccggtcttg ttcctgcccg tgagcgtgcc
     2281 cgcgctgatc gcggccgtgg agacgacgtc cttcgcgctc ggcaacaccg agagcgcttc
     2341 gttctggttc acgcttctga tagtctacga cgtggtgttc gtgaccgcgt cgctgctcgt
     2401 gttcgaatac gtggtggagg aatagatggc tcggcgcttt ggaatggtgc tcgacgccgc
     2461 cttgctcgtc gcgatggcgg ccggcttcgg catgatcttc ttctacgcgc ccatcgagag
     2521 cgagatgggg atcgtgcaga agatcttcta cgtgcacgtg ccgctggcct ggaacgcgtt
     2581 cttgggcttc gcgatcgtgt ttttcgcgag cttccgctat ctcgccacgc gcgaccccaa
     2641 gtgggacgcg cgcgcgctgg cggcggcgga agtgggcgtg ctgttcacaa ccctggtgct
     2701 gatcaccggc cccatctggg ccaagccggt atggggcatc tggtggacgt gggacgcgcg
     2761 gctcacgttg acgctggtgc tgtggctcat ctacgtgggc tatctcatgc tgcgtcgcta
     2821 cgtcgactcg cccgagcggc gcgcggtgct ctcggcggtg gtcggcgtca ccggcttcat
     2881 cgacgttccg ctggtgtact tcgcgattcg ctggtggcgc acccagcacc cgcagcccgt
     2941 gatcgcgggc ggcgaaggct cgggcctcga tccgcgcatg gcgagtacgc tctgggtctg
     3001 cgtcgccgcg ctcacgctct tgttcatcgc cctctaccgg cgccgcctcg cgatcgaggc
     3061 gctgcgcgca cgcgccgacg cgcttcgcga ggaagccgac gccgcgcgcg cggaaggagc
     3121 ccgctcatga gctggttgct gacagcgtac gcggtcgtcg caattgcggt gggcgggtac
     3181 gccctccggt tggcgcgcgt gaagcgcgcg ctggtttccg agatcgacgc cgctacgcgc
     3241 cggcgatagc gggcggcggc cggattgagc acttgcccgg cggtgcccgg gtccatacaa
     3301 tagtagcgtc cgccggcccc cccacccgcc ggcgccggac ttccatgaaa ccgcccaaac
     3361 cgtccaagtc ccccgcgaac tcgccgcagc gcgaaacccg cgtgcaggag gacctcgccc
     3421 tgatcgagat ggtcctgggc gggtcgaccg cgcactggca cgccttcgtc gatcgctatg
     3481 cggggttgat ttacagcgtc attcggcgtc agctcttcgc cgaagaggaa gacgacgtcc
     3541 gcaccgtctt cgccgacgtg ctggaagcgc tctatcgcgg caagctctcc gagttccgcg
     3601 gcagctccga gctctcgacg tggctcatcg tggtgtcgcg cgggaaggcg ctcgaccacc
     3661 ttcgccacat ccagggccgg cgcaagaacc cacagggctg ggacggcctc accccgctcg
     3721 agaagcacgt gttcaagttc cacttcgtcg aagggctcgg cttcgacgcg gtgatccagt
     3781 cgctccgctc ggcgggcgtg cgcgccaacg cggaggcggt cgcgcatgcg gtcttgaaga
     3841 tcgagggcac catcgacaaa cactacctgc gtcggctcga atcggacgcg aaggcacccg
     3901 cgctgggggt ggtgtcgggg cgtctgctcg attacttgaa gcacatgcag ttcgagcgcg
     3961 agcggggcga agaagacccc gacgagagct tcacgcgcga gggcatcgag aggatggcgg
     4021 cgcgcgtgcg cgagctcttg gccgacctct cggacgagga gcgcgacgtg gtccggcttc
     4081 gctacgaaga aggctggacg gcccagcaga tcgcggacga gcgggggctc tcggggcagc
     4141 gcaaggtcta cacgatcctg gacggcgccc tccggaaact ccgaaggctc ctggaccgcg
     4201 acccggaatg agcccggaaa cggccttgga atcggcggac gaggtccgtc tctagaggga
     4261 cgagaaaact cgacgcaggc gccctgggcg ccggaaacca gcagccatga cagagcttca
     4321 cctcaccgat gaacagcttg cggccttggc cgacgactcc atgtcggagt ccgaccgggg
     4381 ccttcttcta gagcacgtcc gctcgtgcaa cgcgtgtcac gatagctact tggacacgat
     4441 ccgctaccgc gccatattgc tcgccgacgc ctcggtgttc cgggcgccgg acgaggtcgt
     4501 gcgcttggcc cggtccatcg ccaggaaggg tgcggcgtac gacgcggtcg aacgccaccc
     4561 gcgcgccgaa gcccggcgcc ggtggttcgc cccgccgctt ctggcgggag cgggtgtggc
     4621 cgtggttgct ctggcggcat tcgcagtttg gcagtccggg gtccgtatcg gcggcaaccg
     4681 ctacgatctc tatttttcgc cccttcaaca ggccgcggcg agcgcctcgg cggaggggtc
     4741 gatcgtgctc ccgggtaccg aggacgcggc cgcggccacg actccgctcc atcggagcgg
     4801 tttcgtagcg gccgacgaga ctatctcgtc cgcgctcgcg cagctcacgc gcgcctatca
     4861 gaacgacgcg aaccccgagg tcgcgcagtg gctgatcagc ggctacttgg cgaccggcga
     4921 gctcgagcag gcgcgcctgt tcgcggagga cgcccggctg cgcttctcgg acgacgcacg
     4981 cttcctggtg ctcgacgcga tcgtcgcgta tcggtccgag gaattcgacc gcgcggaacg
     5041 cctgctgcag gccgcgctgg aggcggagcc gaccaacggc gccgcgcttt tgaacctcgc
     5101 attggtccag tacgagacgg gtcgcgcgga ttccgctcgt cgaaccctcg aactggtccg
     5161 ctcccagttc ccggggtcgc ccctggaggc ccgggcggaa gcgttaatct ccgatctcct
     5221 aaacggttag ccactttctt cggctttttc caagtccctt gggaaattct ctcgggaatt
     5281 cccaccggtc cgtccgaccc tttctcgcag gatcgggtga taagcggttt cgaatccgtc
     5341 taaaggggcg agacgtaacg ggcccgccgc ccggtccaac tcacacagaa cctcccgacg
     5401 cggccgcgca agcgccggtc gggcggacgc gaaaatcgag accccaccac cctcgaactc
     5461 gcggctaacc ggctgcgagt tctttgtgta taatgctcgt cccggcgcca cccaacgccg
     5521 gatcgggatg aacaccgcca cgcacgctca cctgctcccc ctgacattgg cgctaatctc
     5581 gctcgtgatt cccgcggaat cggcgcgacc gtccgaagtg gccctcgtca tccacggcgg
     5641 cgccggcacc atcctgcgcg accaaatgac gcccgagcgc gaagccgccc tgcgcgccgc
     5701 cctcaccgaa gcgctcgaga ccggctatcg cattcttcaa aatggcggct ccagcctgga
     5761 cgccgtcgaa gccgccgtgt gcgtgctcga ggactctcct ctgttcaacg ccgggcgcgg
     5821 cgcggtcttc accagcgaag gcaagaacga gatggacgcc gccatcatgg acggcgccac
     5881 gcgcaaggcg ggtgcggtgg cgtcggtgaa ggggatcaag aacccggtga aagccgcgcg
     5941 cagcgtcatg gaaagaacca gccacgtgat gctggtgggc gaaggcgccg aaaagttcgc
     6001 gcgcgaacaa ggactccagt tcgaagacag cgcgtacttc ttcacacagc accggtggga
     6061 cgagctgaaa cgcacgcagg agaaagagaa agagctcaag aaggagtacg gttcgctcgc
     6121 gtttccgcac acccacctgg gcaccgtggg cgcggtggcc ttggaccaga acggcaacct
     6181 ggcggccgcc acctcgacgg gcgggctgac caacaagcgc tggggccgtg tgggcgactc
     6241 gcccatcatc ggtgcgggta cctacgcgga caacgcgacc tgcgcggtgt cgtgtaccgg
     6301 gcgcggcgag gtcttcattc gcgccgccgc cgcgcacgaa gtctcggccc tgatgcgcct
     6361 cgaaggattc tcggtcgaaa aggccgccaa gcgcgtcgtg cacgaggagc tggtggagct
     6421 gggcggcgag gggacgggcg gcttgatcgc gctcgatcgc aagggccgct tcgcgatgga
     6481 atgcaacacg cccggcatgt accgcggctt cgtcggggcg gacggcaaag cgcgcacctt
     6541 catctaccgc gacgagtaag ccggtgaaag atggctatcc tgcgcctgac gctgacgagc
     6601 cacacggccg aaatcaagcg catcggcggc gcgctgtctg agttcttgga cgaagaaggc
     6661 atccccccct cgctcgcgca ccgcgcccga ctcgtggtcg aagaattggt cgtgaacgtg
     6721 atccggcacg ccttcgacga cgcgcgcgag cacgcgatcc tcttggacgt gcgcaccgag
     6781 ccgggcgggg tggcgatcgt ggtcgaggat gacggcaagg ccttcgatcc gcgcgacgtg
     6841 cgtgccccgg aactgcgcgc gctggtggaa acgggcaagc ccggcggtct cggcttggcc
     6901 atgatcaaga agctcgcgcg cgacctcacg tacgcgcgcg tcgacggcag gaatcgcgtg
     6961 cgcgcgttcg tgggacactc gccgcggggt ggatcgtcgt gacgtcgcgg cgacgcgcga
     7021 ctgcccttct tctcctcctt gtgctggtcg cgtggtgggt ggcgtgcggt gacgacgacc
     7081 ccgtcgaacc ggacaacggc gcgccggcac accgctccga agagatcacc gcgtccaagg
     7141 acaacacgct ctacgaagaa gacgggctct gcgacgcctg ccccggaacc aacggcgtgc
     7201 tcgactcatt gagcaatggt gccggcgagt gggtgttcgc ggggaccaac aacctgggcc
     7261 gcgcgcggcg cgcattggtc gcgttcgacg tcgcgggcaa cattcccgcc ggcgccgtcg
     7321 tcgacagtgt cgtgctgaca ctcaccatgt cgcgcaccat cgccggcacg cacacggtcg
     7381 cactccaccg agtgctcgcc gactggggcg aaggaacctc ggacgcgagc tgctgcgcgc
     7441 ccgaagaggg cacgggcggg accgccaccg tcggcgacgc gacctgggtg ctgcgcgtgt
     7501 tccgcgcggc ggcgtggacg acgcccggcg gcgacttcgc ggtggcttcg agttcggtgc
     7561 ggagcgtggc gggaagcggt ccctaccagt ggcagtcggc gcagatgacg tccgatgtcc
     7621 aggcctggct cgatgcgccc gccaccaact ttggctggat cttgatcggc gacgagaccg
     7681 tggatcgaag cgccaagcgt ttcgattcgc gggagtctgc cgcgaccgcc ccgcgcctca
     7741 cggtctatta caccgtcccc taacccgcgc aagacgcgct tctcgcttcg ccgcgcgtcg
     7801 cgtactctca acccccatgc gcgcgcgatc gttcttattc ggtgttgccg tcgtgctgca
     7861 cgcgcccgtc tcgacgcacg cgcaagcgag tcccttcgac cggctggacg tcgggctcta
     7921 cggcgccgcc aacgtcaatc gcaacgactt ccactcccat tgggacgcgg ggtactcggg
     7981 cgcgatcgac ttcgtgacac cgttctacct cgggcgcgcc tcgttgttgg tgcgcgtggg
     8041 tacgaacgac gccatcgcag gcgcggcgac gtcgggcttc acctcggtgt tcggggcgct
     8101 cggctggcgc gtcgggcggt ctatcgtaga cgaccttcgc accgaccttg ggcttcacgt
     8161 cggcctcacc gagtggatct tctccgaaga ggagcaaacc gaggtccgct acgag
//
LOCUS       DASPGK010000033         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1296953, whole genome shotgun sequence.
ACCESSION   DASPGK010000033 DASPGK010000000
VERSION     DASPGK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296953"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="VEC56_00900"
     CDS             complement(<1..1807)
                     /locus_tag="VEC56_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV12738.1"
                     /translation="MERSQITVAELSSLLRMIQVIPDADNVGRIHQMLLAFCTAWRTI
                     GVRRAFLLLVDQRGRMLRGHLAAEQTVQEPGEGDSATFESLARRVVESTQRIDTGDLT
                     LKTRTFSVPMDWLACGAAKAVNTGVPVLADRRLGEFASDPFFDFFGSVAYVAIPLRLR
                     GVVNAVLVADNADAERPIGVEDISLVYSMSQQAATAMDRLYEAADNSRKFRVLRKLQD
                     ALAAVEDTRRFGEALTAMLSMTARAVGGTGALLKDPLRKKITHVKTVDDVDATDRDTD
                     VSITDAFDDIMDRAAGTQKPVRGDSGHPLLNDAAARVVRYFLALPLLAGGECLGAMAV
                     YVEGFSAGRESDEFSARDRLFFELCAGMMAERLDSLYKAEQVRRTQRMFDEVQSNWFR
                     ERAGTRARQQVEEHHDAVVSQLREVSRTLAGKESAGIRVASARDLLHRIQSEAESFQA
                     EQMAQRESLEMVDLFALVRAVAEEWAGPVRTAGVEVKLRSPERGPVLLMNRENISSAL
                     TNILGILAGHVSKGDRVLIECTTTAEKAVVLIADTAGTVDGSLLSRLFMPFAAAPGDT
                     DPRAAMSIAGDIIQRHAGEITVRSSSSWKTILAVSF"
BASE COUNT          335 a          703 c          692 g          312 t
ORIGIN      
        1 ggaacgagac ggcgaggatc gtcttccacg acgacgacga gcgcaccgtg atctcccccg
       61 cgtggcgctg gatgatgtcg ccggcgatcg acatcgcggc ccgcgggtcg gtgtcgccgg
      121 gagccgccgc gaacggcatg aacagccgcg agagcagcga gccgtcgacc gtacccgccg
      181 tgtccgcaat cagcaccacc gctttctccg ccgtcgtggt gcactcgatc agaacgcggt
      241 cgcccttcga gacgtggccg gccagaatgc cgaggatatt ggtgagcgcg gacgatatgt
      301 tctcgcgatt catcagcagc acggggccgc gctcgggcga gcgcagcttg acctcgacgc
      361 ccgccgtgcg caccgggccc gcccattcct cggccaccgc gcgcaccagc gcgaacaagt
      421 cgaccatctc gagcgactcg cgctgcgcca tctgctcggc ctgaaacgat tccgcctccg
      481 actggatgcg atgcaggaga tcgcgcgcgg acgcgacgcg aatgcccgcg gattccttcc
      541 cggcgagcgt gcgcgacacc tcgcgcagct gcgacaccac ggcgtcgtgg tgttcctcga
      601 cctgctggcg cgcgcgcgtc cccgcgcgct cgcggaacca gttcgactgc acctcgtcga
      661 acatgcgctg ggtgcgtcgc acctgctcgg ccttgtagag cgaatcgagc cgctccgcca
      721 tcatcccggc gcacagctcg aagaagaggc ggtcgcgcgc ggagaactcg tccgactcgc
      781 gcccggccga aaacccctcc acgtacaccg ccatcgctcc caggcactcg ccgcccgcga
      841 gcagcgggag cgcgagaaaa tagcgcacca cgcgcgcggc ggcgtcgttc aacagcgggt
      901 ggcccgagtc gccgcgcacc ggcttctggg tgcccgcggc gcgatccatg atgtcgtcga
      961 aggcgtccgt gatggagacg tcggtgtcgc ggtcggtggc gtctacgtcg tcgacggtct
     1021 tgacgtgcgt gatcttcttg cgcagcgggt ccttgaggag cgcgccggtc ccgcccaccg
     1081 cacgcgccgt catcgacagc atcgcggtca atgcctcgcc gaaacgccgc gtgtcctcca
     1141 cggccgccag cgcgtcctgg agctttcgca gtactcggaa tttgcgcgag ttgtcggccg
     1201 cttcgtagag acggtccatc gcggtggccg cctgctgcga catgctgtag acgagcgaga
     1261 tgtcttcgac gccgatgggg cgctccgcgt cggcgttgtc cgcgaccagc accgcgttga
     1321 cgacgccgcg cagccgcaaa ggaattgcga cgtaggcgac cgaaccgaag aagtcgaaga
     1381 aggggtcgct agcgaactcg cccaggcgcc ggtcggcgag gacgggaacg ccggtgttga
     1441 ccgccttggc cgcgccgcac gcgagccagt ccatcggcac gctgaaggtc cgcgtcttga
     1501 gggtcaggtc gccggtgtcg atgcgctgcg tgctctccac gacgcgacgc gcgaggctct
     1561 cgaaggtcgc ggagtcgcct tcaccgggtt cctgaaccgt ctgctcggcc gcgaggtgcc
     1621 cgcgcagcat gcgcccgcgc tggtcgacca ggagcaggaa cgcgcgccgc acgccgatgg
     1681 tccgccacgc ggtgcagaac gcgagcagca tctggtggat gcgcccgacg ttgtccgcgt
     1741 cgggaatcac ctggatcatg cgcaagagcg aggagagctc ggcgacggtg atttgggatc
     1801 gttccatggc ctcgggacgg aatcggcgtg cgacgacggt aaagatgccg tccccaggaa
     1861 cgcaagcttt gtgccacggg gtagatgctc acgacaccgc gcggaccccg agcctaggct
     1921 cgaatcgcgc gtcggtggtg cttctcgtag agcaggtgga cgatgccatc ggagagcccg
     1981 acctccggga ccaggatctt ggagatcttg ccccacttca tcaccgacag atagacgtcg
     2041 ga
//
LOCUS       DASPGK010000034         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_125904, whole genome shotgun sequence.
ACCESSION   DASPGK010000034 DASPGK010000000
VERSION     DASPGK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125904"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="VEC56_00905"
     CDS             <1..1643
                     /locus_tag="VEC56_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXV12739.1"
                     /translation="DYFLFDRVREGRGPHPALRYGARSYSYDDVAARARALASYFAAA
                     GVAPEARVYIVLPDAPPFAWSFFATLAHGGVVTMGNSIAPRDDLAYVIEYVRAAVLIT
                     VPQVAADLAAAIARAPHLQCVLLAPDVATGEDPEGACEIPSSLRGGRARVDTVVDAIA
                     AGTRAPRALPDVRRDDPAIWLFTSGSTGRPKAATHTHRDFAYNTEVYAKRTVGYRADD
                     VTVSVPRLFFGYATGTNLMFPFAVGATAALFSERPTAESLAHAIAMYRPTVVTNVPTM
                     MSKLLEHDDALRARGESVLDFRSIRFHLSAGEALPPALLERFRARFGNDVYDGIGSAE
                     MFHIYASNRPGDIKAGSLGRAVEGYEIRILPTDALEPGAAEVARGDVGVMWVRGDSVA
                     MSYHGDRDKSWETFHGAWCRTGDLFRMDDEGYLWFCGRADSLFKVGGVFVAPLEIEEC
                     LMEHEAVALAAVIPAEEAGLVKPKAFVALREGVAARDDGAREALRRALQEHVKERLSK
                     HKYPRWVVFVDDLPKNDRGKIDRKVLIDRERAGENPWR"
     gene            1640..2398
                     /locus_tag="VEC56_00910"
     CDS             1640..2398
                     /locus_tag="VEC56_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXV12740.1"
                     /translation="MSGRYESVFVARLTTEQFDAAIKNRPVVILLPVGAVEPHGPHLP
                     LDTDLIISRAAAERATHALWARDVLAFVAPDVAYGVTTFARDFSGAAGVSADALAAYL
                     RSVVEGWLATGVAHVCVVNNHLEPAHDAAVRDAIAPLAHAHASVASPLTRRWARTLSD
                     EFKRGACHAGEYETSIVQAADSDSVREAIRRTLPEVDVSLSDAIARGVDRFVDMGLSR
                     GYAGAPARATPAHGEEMLARLAEMIVGEVTEAMG"
     gene            complement(2406..3380)
                     /locus_tag="VEC56_00915"
     CDS             complement(2406..3380)
                     /locus_tag="VEC56_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXV12741.1"
                     /translation="MKRKLLIGTLVSLVFLYLAVRGISWSEFVDTMRRTRWTLLVPAV
                     AATMLGHFSRSLRWKFMMSPIKECRIGPLWSATAIAFMVNNLLPARLGEFVRAIAIGR
                     TERVSKSASFATIVYERVVDVFVLIILIWYCSVRVSAPGWLARSTEILIVFNVALFVL
                     LFVMVRWRRRFRALLARAVRPLPTEAQRRIHGSADAFIDGLGVVTKPSSALPITLLSV
                     AVWGFAVLGVYFSALAMNLTLPFLASVFLIVLVSLGSMIPSAPAFLGTIQYACVLGLG
                     LYHVDRGQALAFSTVYHATQFFPITLTGLYYAWRSHLRLSELSRGAIE"
     gene            complement(3481..4416)
                     /locus_tag="VEC56_00920"
     CDS             complement(3481..4416)
                     /locus_tag="VEC56_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV12742.1"
                     /translation="MYREKSVSVVIPAYNVAAHVADVVAGIPAFVDHIFVVNDASRDG
                     TADALAAVTDPRARIITLEKNQGVGGATVRGFREALAAGSDIVVKMDADGQMDPAYLG
                     ALIDPVAHGHFDYTKGNRFLDGAALARMPKVRLFGNLVLTFLTKLASGYWHIFDPQNG
                     YIAIDGTMLSRLPLERLSRRYFFENDMLVHLNIARAHVEDISIPARYGEEDSALRVST
                     VLLTFPPRLMQRFAYRVYQRHVLRDFSIVAVFWFFGLALLAWGVGFGLLVWGTSISSG
                     KPATTGTVMLSALPLILGVQFILQAIVIEVNESRS"
     gene            complement(4467..>4753)
                     /locus_tag="VEC56_00925"
     CDS             complement(4467..>4753)
                     /locus_tag="VEC56_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12743.1"
                     /translation="RRHHAVRALYAEAPLDSTTLRVLADAEAPLYVIVRPEHRRAGAA
                     LWAREDLFPIAFDDGTIQVREVNRDACRAAMSGAASPPLDEELIRASGLY"
BASE COUNT          834 a         1565 c         1618 g          736 t
ORIGIN      
        1 ccgactattt tctgttcgac cgcgttcgcg aaggccgcgg accccacccc gcgctgcgct
       61 acggcgcgcg ctcgtactcg tacgacgacg tcgccgcgcg cgcacgcgcc ctagcgagtt
      121 atttcgccgc ggccggcgtc gcccccgaag cgcgcgttta catcgtgctc ccggacgcgc
      181 ctcccttcgc atggtcgttt ttcgccaccc tcgcccacgg cggcgtggtg acgatgggga
      241 actcaatcgc gccgcgcgac gacctcgcgt acgtgatcga atacgtgcgc gcggcggtat
      301 tgatcacggt gccgcaggtg gccgccgacc tcgccgctgc gatcgcgcgc gcgccgcacc
      361 tccagtgtgt tctcttggcg cccgacgtcg ccaccggcga agatcccgaa ggcgcgtgcg
      421 agattccgtc gtcgctgcgc ggaggacgcg cgcgcgtcga caccgtggtc gacgccatcg
      481 ccgccgggac gcgcgcgccg cgtgctctcc ccgacgtccg acgcgacgac cctgcgatct
      541 ggctcttcac ctccggttcg accggtcgcc ccaaggccgc gacccacaca caccgcgact
      601 tcgcgtacaa caccgaggtg tacgccaagc gcacggtggg ttatcgcgcg gatgacgtta
      661 ccgtcagcgt gccgcgcctg ttcttcgggt acgccaccgg caccaacttg atgttcccgt
      721 tcgcggtggg ggcgacggcg gcgctcttct ccgagcgccc gaccgcggag tcgctcgccc
      781 acgccatcgc gatgtaccgt cccacggtgg tgaccaacgt gcccaccatg atgtcgaagt
      841 tgctcgagca cgacgacgcg ctgcgcgcgc gcggcgaatc cgtgctcgac ttccgctcga
      901 ttcggttcca cctatcggcc ggggaggcgc tcccgcccgc gctgctcgag cgtttccgcg
      961 cgcgctttgg caacgatgtc tacgacggga tcggctcggc ggagatgttt catatctacg
     1021 cgtccaatcg ccccggggac atcaaggcgg gctcgttggg gcgcgcggtg gagggctacg
     1081 agattcgcat tcttcccacc gacgcgctgg agcccggggc cgcggaggtc gctcgcggcg
     1141 acgtcggtgt gatgtgggtg cgcggcgaca gcgtcgccat gagttatcac ggcgaccgcg
     1201 ataagagttg ggagaccttc cacggcgcgt ggtgccgcac cggcgatctg ttccgcatgg
     1261 acgacgaggg ttatctgtgg ttctgcgggc gcgcggacag cctgttcaag gtgggcggcg
     1321 tgttcgtggc cccgctcgaa atcgaggagt gcctgatgga gcacgaggcg gtcgcgctgg
     1381 cagcagtgat cccggccgaa gaggccggcc tcgtcaagcc gaaggcgttc gtcgcgctgc
     1441 gcgagggtgt ggccgcgcgc gacgacggcg cgcgcgaggc gctgcgccgc gcgctccagg
     1501 agcacgtgaa ggagcgtctc tccaagcaca agtatccgcg ctgggtcgtg ttcgtcgacg
     1561 acctgcccaa gaacgatcgg ggtaagatcg atcgcaaggt gctgatcgac cgcgagcgcg
     1621 cgggggagaa tccatggcga tgagcggtcg atacgagagc gtgttcgtcg cgcggctgac
     1681 gaccgaacaa ttcgacgccg cgattaagaa ccggccggtg gtcatcctgc tcccggtggg
     1741 agcggtcgag ccccacggac cgcacttgcc tctcgacacc gatttgatca tctcgcgtgc
     1801 ggcggcggaa cgggccacgc acgcgctctg ggcccgcgac gtgctcgcct tcgtcgcccc
     1861 cgacgtcgcc tacggcgtca cgaccttcgc gcgcgacttt tcgggcgctg ccggggtctc
     1921 cgccgacgcg ctggcggcgt acctgcggtc ggtggtggag gggtggctcg cgacgggcgt
     1981 ggcccacgtg tgcgtggtca acaaccacct cgagcccgcc cacgacgccg ccgtgcgcga
     2041 cgcgatcgct cctcttgcac acgcgcacgc ctcggtggcg agcccgctca cccgccggtg
     2101 ggcgcgcacg ctctccgacg agttcaagcg cggcgcctgt cacgcgggcg agtacgagac
     2161 gtcgatcgtg caggcggcgg atagcgactc ggtgcgcgag gcgattcggc gtacgcttcc
     2221 cgaggtcgac gtgagcctgt cggatgcgat cgcgcgcggc gtcgatcgct tcgtcgacat
     2281 gggactctcc cgggggtacg cgggcgcgcc cgcgcgcgcc acacccgcgc acggcgagga
     2341 gatgctggcg cgcctcgcgg aaatgatcgt gggggaagtg acggaagcga tgggttagcg
     2401 cgcgctcact cgattgcgcc gcgcgacagt tccgacaggc gcaggtgcga tcgccaggcg
     2461 taatacagcc ccgtcagcgt gatcgggaag aactgcgtgg cgtggtatac ggtcgagaac
     2521 gcgagcgcct ggccgcgatc cacgtggtag agccccagtc ccagcacgca cgcatactga
     2581 atcgtcccga ggaacgcggg cgccgacgga atcatcgatc cgagcgaaac cagcacgatg
     2641 aggaacacgc tggccagaaa cggcagcgtg agattcatcg ccagcgcgga gaagtagacc
     2701 cccagcaccg cgaaccccca caccgccacc gacaggaggg tgatcggcag ggccgaggac
     2761 ggcttggtta cgactccgag cccatcgatg aaggcgtcgg cggagccgtg gatacggcgt
     2821 tgcgcctcgg tgggcagcgg tcgtaccgcc cgggcgagca gcgcgcggaa gcgccggcgc
     2881 cagcgtacca tgacaaaaag cagaacgaac aacgccacgt tgaacacgat cagaatctcg
     2941 gtactgcgag cgagccagcc cggggcggat acgcgcaccg agcagtacca gatcaggatg
     3001 attagcacga atacgtcgac cacgcgctcg tagacgatgg tcgcgaacga cgccgacttg
     3061 ctcacgcgct ccgtgcgccc gatggcgatg gcgcgcacga attcgcccag ccgtgccggg
     3121 agcagattgt tgaccatgaa cgcgatcgcg gtcgccgacc acaggggccc gattcggcac
     3181 tccttgatgg gcgacatcat gaacttccag cgcagcgagc gcgagaagtg accgagcatc
     3241 gtggccgcga ccgccgggac tagcaatgtc caacgagtcc ggcgcatcgt gtctacgaac
     3301 tcgctccacg agatgccccg cacggcgaga tagaggaaca ccagactcac caaagtgccg
     3361 atgaggagct ttcgtttcac ggctcggtca gccccggtcg tgcgtggatg gaggcaatct
     3421 cgagcaatcg ctcgctgccc ccggcggcgt cgcgttgcga cctgcaacga gcgtcggagt
     3481 tcatgagcgc gattcgttta cctcaatgac gatggcctgg agtatgaact ggacgccgag
     3541 gatgagcgga agcgcgctca gcatcacggt gccggtggtg gcgggcttgc ccgaggagat
     3601 cgaggttccc cataccagca atccaaagcc gactccccag gccagcaagg caaggccaaa
     3661 gaaccagaat accgctacga tcgagaaatc gcgcaggaca tggcgctggt acacgcggta
     3721 cgcaaagcgc tgcatgagac ggggcgggaa cgtcaacaga acggtgctaa cccgcaaggc
     3781 ggagtcttct tcgccatagc gcgccggaat ggaaatatcc tccacgtgag cacgagcgat
     3841 gttcaggtgg accagcatgt cgttctcgaa gaagtagcgt cgcgataaac gctcgagggg
     3901 aaggcgcgac agcatcgttc cgtcgatggc gatataaccg ttctgcggat cgaagatatg
     3961 ccagtaaccg gatgcgagtt tggtgagaaa ggtgagcacg agattgccga aaagacgcac
     4021 cttcggcatg cgcgcgaggg cggctccgtc caagaagcgg ttgcccttgg tgtagtcgaa
     4081 gtgcccatga gcgacgggat cgatcagggc gcccaggtac gcggggtcca tctgtccgtc
     4141 cgcgtccatc ttcaccacga tatcggagcc ggcggcgagc gcctcccgga aaccccgcac
     4201 ggtcgcgccg ccgacgcctt gattcttctc caaggtgatg attcgagctc gtggatcggt
     4261 gaccgcggcc agcgcatccg cggttccatc gcgcgaggcg tcgttcacga cgaatatgtg
     4321 gtcaacgaac gccggaatcc cggccaccac gtcggcgacg tgagccgcga cgttgtacgc
     4381 gggtatcacg accgagacgg acttctccct atacacgggc gagcctccgc gggatgggcc
     4441 gggctcgatg cccgggagcg cggcggtcaa tagagtcccg acgcacggat caactcctcg
     4501 tcgaggggcg gggaagccgc tccgctcatc gcggctcggc acgcatcacg gtttacctca
     4561 cgcacctgga tcgtgccgtc gtcaaacgca atcggaaaca gatcctcgcg cgcccacagc
     4621 gcggcacctg cgcggcgatg ctcggggcgc acgatgacgt agagaggggc ctccgcgtcc
     4681 gcgagcacgc gaagtgtcgt cgagtcgagc ggggcttcgg cgtacaatgc gcgcaccgcg
     4741 tgatggcgtc ggg
//
LOCUS       DASPGK010000035         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1648973, whole genome shotgun sequence.
ACCESSION   DASPGK010000035 DASPGK010000000
VERSION     DASPGK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648973"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEC56_00930"
     CDS             <1..226
                     /locus_tag="VEC56_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HXV12744.1"
                     /translation="WRSAGTVGNGRVHLFENDTGPDDANHAPEGIFIWHNRGKQAAGS
                     TERVSIYDVAPSILDFYSIPAPADMIGRTL"
     gene            252..851
                     /gene="cysC"
                     /locus_tag="VEC56_00935"
     CDS             252..851
                     /gene="cysC"
                     /locus_tag="VEC56_00935"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXV12745.1"
                     /translation="MKGFTLWFTGLPSSGKSTLARRVEEILLERGMNVEVLDGDEVRE
                     NLSKGLGFSKQDRDTNIRRIGFVARLLSRNGVVAITAAISPYADVRDEMRGAIGRFVE
                     VYVKCPIDVLKERDVKGLYKKALAGEIKHFTGVDDPYEAPARPEVLIESDRETIDQSV
                     AKIIQTLEVMGYVPRGDGGQYSAQDEEAIKTRLKDLGYI"
     gene            859..>2045
                     /locus_tag="VEC56_00940"
     CDS             859..>2045
                     /locus_tag="VEC56_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV12746.1"
                     /translation="MAVKWHQQPTQRACVVGLDGVPCSMMRRFIDAGVTPRIGEIARS
                     GALHDMTVSLPEISSVSWSTFMTGKNPGEHGIFGFTDLHAGTYTQSFPSFRDLKTDTI
                     WDRLGEHQMRSVVINQPATYPARPIHGVLVSGFVAVHLDKSVFPARYLRTVRDLDYRI
                     DLDLEEVRDRPDLLFPRLHDLLRVREQLMGILWAEEKWNLMQVVVTGTDRLHHFLFDA
                     FEDEAHPYHGAFLDYYRAVDGFVGRVYDRFAADPEAKGFFVLSDHGFCRTRHEVNVNA
                     VLAKSGYLAFTNPGAAAIDAVSERATAFALDPARIYLHKRGKYPRGGVAPERETALLS
                     ELADLFSSLTYQGERVVRHVFYGRDVYAGPETPHGPDLLLVPTPGFDLKGRMGSAEVV
                     VARR"
BASE COUNT          371 a          688 c          631 g          355 t
ORIGIN      
        1 ctggcgcagc gcgggcaccg tcggcaacgg tcgtgtccat ctgttcgaga acgataccgg
       61 acccgacgac gccaaccacg cgccggaggg gatcttcata tggcacaatc ggggtaaaca
      121 agcggcgggg tccaccgaac gcgtgtccat ctacgacgtg gcgccctcga ttttggactt
      181 ctattcgatt cccgcgccgg ccgacatgat cggtcggacg ctgtagcgca cggcaactca
      241 acggaggact catgaagggc ttcacactgt ggtttacggg gcttccctct tcgggcaagt
      301 ccacgctcgc gcgccgcgta gaggaaatcc tgctcgagcg cggtatgaac gtcgaagtac
      361 tggacggcga cgaagtgcgc gaaaacctga gcaaaggact cggcttctcg aagcaggacc
      421 gcgacaccaa cattcgccgc atcggtttcg tggccaggct tctgtcgcgc aacggcgtgg
      481 ttgccatcac cgccgccatc tcgccctacg ccgacgttcg cgacgagatg cgcggcgcca
      541 tcgggcgctt cgtcgaggtg tacgtcaagt gccccatcga cgtgctcaag gagcgcgacg
      601 tcaaagggct gtacaagaag gcgctggcgg gagagatcaa gcacttcacc ggcgtcgatg
      661 atccctacga agcgcccgcc cgccccgagg tgctgatcga gagcgaccgc gaaacgatcg
      721 accagagcgt ggccaagatc atccagaccc tcgaggtcat gggctacgtt ccgcgcggcg
      781 acggcgggca gtactcggcc caggacgagg aagcgatcaa gacccgattg aaggacctcg
      841 gctacatcta gtctcgccat ggcggtgaag tggcaccagc aaccgactca gcgcgcgtgc
      901 gtcgtcggac tcgacggcgt gccgtgctcg atgatgcggc gtttcatcga cgccggagtg
      961 acgccgcgca tcggtgagat cgcgcgctcc ggtgcgctcc acgacatgac ggtgtcgctt
     1021 ccggagattt cgtcggtgag ctggtccacg ttcatgaccg gcaagaaccc gggcgagcac
     1081 ggtattttcg gcttcaccga tttgcacgcg ggcacctaca cccaaagctt tcccagtttc
     1141 cgcgacctca agaccgacac catctgggac cgcctcggcg agcaccaaat gcgcagtgtt
     1201 gtgatcaacc agcccgccac ctaccccgcg cgccccattc acggcgtgct cgtgtcgggc
     1261 ttcgtcgcgg tgcatctcga caagtcggtg ttccccgcgc gctacttgcg aaccgtgcgc
     1321 gacctcgact atcgcatcga cctcgacctc gaggaagtgc gggaccgtcc cgatctgctc
     1381 ttccctcgcc tccacgacct cctgcgcgtg cgcgagcaac tcatgggcat cctgtgggcc
     1441 gaagaaaagt ggaatctcat gcaggtggtc gtcaccggaa ccgatcgcct gcaccatttc
     1501 ctcttcgacg ccttcgaaga cgaagcgcat ccgtatcacg gcgccttcct cgactactac
     1561 cgcgcggtcg acgggttcgt cgggcgtgtg tacgaccgct tcgccgccga ccccgaggcc
     1621 aagggctttt tcgtattgtc cgaccacgga ttctgccgca cgcgccacga ggtcaacgtc
     1681 aacgcggtgc tcgcgaagag cggatatctc gcgttcacga atcccggggc ggccgcgatc
     1741 gacgccgtct ccgagcgtgc gactgcgttc gcgctcgatc ccgcgcgcat ctacctccac
     1801 aagcgcggca agtatccgcg cggcggggtc gcaccggaac gcgaaacggc actgctctcc
     1861 gaactggccg acctcttctc ctcgctcacg taccagggag agcgcgtcgt tcggcacgtg
     1921 ttctacgggc gcgacgtata cgcgggaccg gagacgcccc acggtcccga tttgctgctc
     1981 gtgcccaccc ccggcttcga cttgaagggc cgcatgggaa gcgcggaggt cgtggtagcg
     2041 cggcg
//
LOCUS       DASPGK010000036         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_711995, whole genome shotgun sequence.
ACCESSION   DASPGK010000036 DASPGK010000000
VERSION     DASPGK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711995"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..2240)
                     /locus_tag="VEC56_00945"
     CDS             complement(15..2240)
                     /locus_tag="VEC56_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXV12747.1"
                     /translation="MNDPTKPTGTGDTPGQPRAPEASGAHTPAYVPPAPEAPGSGGKP
                     PFWRRWTPRQLALGAAVLVAGVLFSAYQYFASDLPSTSRLEMIEPTLKTVVYGADSTV
                     VGEFFVEDRALVPLADLPPHMVDAFVSVEDRKFYSHWGVDLFGIMRAVVKNVMAGETV
                     QGGSTITQQLGRNLFDMFEDTLTRKIKEAMLAMRIERAYSKDEILEMYLNQIYFGGGA
                     HGVEAAAQTFFGKSARELTVGESSLLAGLPKNPSRYSPINHPENAMQRRTVVLNAMVD
                     TRKLTRAEADSIDATPVNIRPGKVGRSEFAAYFLETIRQYLEEKYGADRIYRDGLKVY
                     TGLDPAMQRAAEDSMESHLRRIETARGYKQTRARYEQELAKGEELGPPHYLQGAVVAI
                     DVKTGLVRAMVGGRSYKHSKFNRAVQAKRQPGSSFKPFIFCAAIENGYTPADILLDAP
                     IVLDLPNGDVWKPQNYSGTFEGEVTVRHALNSSINIAAIRLLMAMGPGEAINVAHRLG
                     VKSDLENVYSLALGVSEVTLLEITNAYASIAAGGMRGEPILLYRLEDREGRVLEENTI
                     LREEVLDPKVNYMITSLLGSVMNEGFGRGARIAGFHEPAGGKTGTTDLNTDAWFVGFT
                     QDIAVGVWTGFDEKKTMGDGMTGGAVSLPTWTAVMKTAYRNGRHAPAFPVPEGIESRV
                     ICELTGLLATEACPKIRREVFIAGDEPQRACDRHGSASSRPVQDLQTLEEIDREILED
                     G"
     gene            2461..3702
                     /gene="tyrS"
                     /locus_tag="VEC56_00950"
     CDS             2461..3702
                     /gene="tyrS"
                     /locus_tag="VEC56_00950"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXV12748.1"
                     /translation="MTRKHAVDPARIDAQLAVIRQGAVEIIPEADLRRKLERSLSANE
                     PLVIKQGFDPTAPDIHLGHTVGLRKLRQFQDLGHKVVFLIGDFTGMIGDPSGRNETRK
                     RLDHQVLVENAKTYTEQAFKILDPKKTIIDYNSRWLAKMEFADVLRLTATQTVAQMLE
                     RDDFEKRYAAGKPISLLEFVYPLAQGYDSVVLNADVEIGATEQKFNLLMAREIQRQYG
                     QEPEVIITLPILEGTDGVEKMSKSLGNYIGVADSPEDIFGKTMKIPDSLIVKYFELVS
                     TKSPAELDAVRARMQDASVNPSHLKRELARDLVLQFHDAEAAQAAEAHFNRLFVEHQR
                     PQETTRVDVKSAEGGVWIAKALTQAGLCESTSAARRMIQQGAVKVDGAKVSDVDLRLS
                     ARKEPYAIQVGKRGFADVVVS"
BASE COUNT          627 a         1236 c         1202 g          652 t
ORIGIN      
        1 gttcggccga gtgtttagcc gtcttccaga atctcgcgat cgatctcttc cagcgtttgc
       61 aggtcttgca cggggcgcga cgacgccgaa ccgtggcggt cgcacgcgcg ctgcggttcg
      121 tcgcccgcga tgaacacttc gcgccggatc ttggggcacg cttcggtggc gagcagcccc
      181 gtcagctcac agatcacgcg cgactcgatg ccttcgggga ccgggaacgc cggcgcgtgg
      241 cggccgttgc ggtacgcggt tttcatgacg gccgtccacg tgggcagcga gaccgcgccg
      301 cccgtcatgc cgtcgcccat ggtcttcttc tcgtcgaagc cggtccacac ccccaccgcg
      361 atatcctgcg tgaaccccac gaaccacgcg tccgtgttga ggtcggtcgt gccggtcttg
      421 ccgcccgcag gctcgtggaa gcccgcgatg cgcgcgccgc gcccgaaccc ttcgttcatc
      481 acgctgccca agaggctcgt gatcatgtag ttcaccttcg ggtcgagcac ctcctcgcgc
      541 aggatggtat tttcctcgag cacgcggccc tcgcggtcct ccaaacgata gagtaggatc
      601 ggctcgccgc gcatgccgcc agcggcgatg ctcgcgtacg cgttggtgat ctcgagcagc
      661 gtcacttcgg agacgccgag agccagcgag tacacgttct cgaggtccga cttcacaccc
      721 aggcggtgcg cgacgttgat cgcttcgccc ggacccatcg ccatcagcaa gcgaatcgcc
      781 gcgatgttga tcgacgaatt gagcgcgtgg cgaaccgtga cctcgccctc gaaggtgccc
      841 gagtagttct gcggcttcca cacatcgccg ttggggaggt cgagtacgat gggcgcatcg
      901 aggaggatgt cggcgggcgt gtagccgttc tcgatggccg cgcagaagat gaacggcttg
      961 aacgagctgc ccggctgccg cttggcctgc accgcgcggt tgaacttgct gtgcttgtag
     1021 ctgcggccgc ccaccatcgc gcgtaccaga ccggtcttga cgtcgatcgc aaccaccgcg
     1081 ccctgcaggt agtgcggggg ccccaactcc tcgcccttgg ccagctcttg ctcgtagcgc
     1141 gctcgcgtct gtttgtagcc gcgcgcggtc tcgatgcgtc gcaggtgcga ttccatgctg
     1201 tcttcggccg cgcgttgcat ggccggatcc agccccgtgt acaccttgag gccgtcgcgg
     1261 tagatgcggt cggcgccgta tttctcttcc aagtactgcc gaatcgtctc gaggaagtag
     1321 gccgcgaact cgcttcgtcc caccttgccg gggcgaatgt taaccggggt ggcgtcgatc
     1381 gagtcggctt ccgcgcgcgt gagcttgcgc gtatccacca tcgcgttgag cacgaccgtc
     1441 cgccgctgca tggcgttttc ggggtgattg atgggcgagt accggctcgg attcttcggc
     1501 aatcccgcga gcagcgagga ctctcccacc gtcagctcgc gcgccgactt cccaaagaac
     1561 gtctgcgcgg cggcttcgac gccgtgcgcg ccgcccccga aataaatctg attgagatac
     1621 atctccagga tctcgtcctt ggagtacgca cgctcgatgc gcatcgccag catcgcttcc
     1681 ttgatcttgc gcgtcagcgt gtcctcgaac atgtcgaaca ggttgcgccc gagctgctgg
     1741 gtaatggtgc tgccgccctg cacggtctcg ccggccatga cgttcttcac caccgcgcgc
     1801 atgattccga acaagtcgac cccccagtgg gagtagaatt tccggtcttc gaccgaaacg
     1861 aaggcgtcaa ccatgtgggg ggggagatcg gccaggggaa ccagcgcgcg atcctcgacg
     1921 aagaattcgc ccaccacggt ggaatcggcg ccgtagacca cggttttgag cgtcggctcg
     1981 atcatctcga gtcgcgacgt gctggggagg tcgctcgcaa aatactggta ggccgaaaag
     2041 agcacgcccg ccacgagcac ggcggctccc aacgccagct ggcgcggcgt ccagcgccgc
     2101 caaaacggcg gctttccgcc ggagcccggc gcttccggcg ccggtggaac gtaggcgggc
     2161 gtgtgcgccc ccgaggcctc cggggcgcgc ggctggccgg gagtgtctcc ggtgccggtc
     2221 ggtttcgtgg gatcgttcat gttcgtccga ctgatcgggg ccgatccggg tcgccccgca
     2281 ccatagcatg gggtcgctgg acccggccac gctttacggg agctcacacc cgttgatata
     2341 atcccccgat tcgactcaag ttataccccg gccgcgcggg ctcggccggc cttcggccgg
     2401 cctgggggcc tttctcgcga acccgggccc ggctttttcc gtggcggagg tgccaccggc
     2461 atgacccgaa agcatgcggt cgatcccgcg cgcatcgacg cccaattggc cgtcattcgc
     2521 cagggagcgg tagagataat ccccgaggcc gacctgcgcc gcaagctcga gcgatcgctc
     2581 tcggccaacg agcccctggt gatcaagcag ggcttcgacc cgaccgcacc cgacattcac
     2641 ctcggccaca cggtcggctt gcgaaagctg cgccaattcc aggacctggg ccacaaagtc
     2701 gtgttcctga tcggggactt caccggcatg atcggcgacc ccagcgggcg caacgagacc
     2761 cgcaagcgcc tggatcacca ggtgctggtc gagaacgcca agacctacac cgagcaggcc
     2821 ttcaagatcc tggatcccaa gaagaccatc atcgactaca acagccgctg gctggccaag
     2881 atggagttcg ccgacgtgct gcgattgacg gcgacgcaaa cggtggcgca gatgctcgag
     2941 cgcgacgact tcgagaaacg ctacgcggcc ggcaagccca tcagcctgct cgaattcgtc
     3001 tacccgctgg cccagggcta cgactcggtg gtcttgaacg ccgacgtcga gatcggcgcc
     3061 accgagcaga agttcaacct gctcatggcg cgcgagatcc agcgccaata cggccaggag
     3121 ccggaagtga tcatcacgct gccgatcctc gaaggcaccg acggggtgga gaagatgagc
     3181 aagtcgctcg ggaactacat cggcgtggcg gattctcccg aggacatttt cggaaagacc
     3241 atgaagattc ccgactcgct catcgtcaag tacttcgagc tggtgagcac gaaatccccc
     3301 gccgagttgg atgccgtccg cgcgcgcatg caggacgcgt cggtcaaccc gtcgcacctg
     3361 aagcgcgagt tggcgcgcga tctcgtcctt cagttccacg acgcggaggc ggcgcaggcg
     3421 gcggaggcgc acttcaatcg cttgttcgtc gagcaccagc gcccgcagga aaccacccgc
     3481 gtcgacgtga agtcggccga aggcggggtg tggatcgcga aggcgctgac gcaggccggg
     3541 ctgtgcgaga gcacgtcggc ggcgcgtcgc atgattcagc agggagcggt caaggtcgac
     3601 ggggcgaagg tgagcgacgt cgatctgcgc ctgtcggcgc gcaaggagcc ctacgccatc
     3661 caggtgggca agcgcggctt cgcggacgtc gtcgtgtcgt gagacgcgcg ctccgct
//
LOCUS       DASPGK010000037         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_829375, whole genome shotgun sequence.
ACCESSION   DASPGK010000037 DASPGK010000000
VERSION     DASPGK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829375"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VEC56_00955"
     CDS             <1..886
                     /locus_tag="VEC56_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12749.1"
                     /translation="ISPWIYRVGTFVDFGRLATPVRPGQLDTAERLRGDTTFRFEAVP
                     YAAFAYGVGFTLGPSLRELHETTSLAGVFALHGAVIVWVGALFGALLVRGVARGARDH
                     GPWSVVEPLLYVGIPLLATLALNWQNAKAFNVRYVLVGLPMYLALVALGVASLPRARE
                     ALAGTLVAATCAVSLWNYYTDPRYAKEDVRSAVRAIEARIAPGECILAPTVWQIVEVY
                     ATTDAPVLNVYGSPESTMQDQLGRLVVQCDSFWYVRARTWVDDPQGRVLREIDARYVR
                     GETWEFPGVSASHFARRR"
     gene            1026..1523
                     /locus_tag="VEC56_00960"
     CDS             1026..1523
                     /locus_tag="VEC56_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12750.1"
                     /translation="MRPRHPYSSIALAIAVALCASCAASPPRRDATPEIQAIRDDYLR
                     ANPDVVYKDAIARGEVVVGMGYQDVLAAWGRPTARVVQQGTDDAERWTYVLVSDNGTD
                     WVRYDFFFAKWSVVQWETTRSVASGFAPARDDARGVSTRVPPMPAPAALGEGVRKGGA
                     GSMIR"
     gene            1695..2168
                     /locus_tag="VEC56_00965"
     CDS             1695..2168
                     /locus_tag="VEC56_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV12751.1"
                     /translation="MNTRLPHAPAWPLSRELDMSEFDNIRELLEMLMSEHTPAKHKIK
                     MKTSFGWEPAMNVFETDGEFVVIMDIAGMDPKEITVFTDGNILRISGVRKDLIPPGKK
                     QFHALEIQVGPFQRLIGIPVPVDRDSVTTHYANGLLEIRLKKRGDHPGKRRVEIE"
     gene            2194..>3782
                     /gene="lon"
                     /locus_tag="VEC56_00970"
     CDS             2194..>3782
                     /gene="lon"
                     /locus_tag="VEC56_00970"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXV12752.1"
                     /translation="MKSRSTLEFEEPGVSTGSIPSELAILPINDAVVFPLMMVPLILR
                     DPNLLRLADEALAEHKIIGAFTQKNAETEHPGPDDIYRVGTAIHIQKMIRFPNGEMRL
                     LAQGIARIQLTEITQVVPYLKGKVRPLDEIESDTDLHKAYVQNVVRTFSKMIDESDTL
                     ADELKIIINNIKEGGRLADVIATNLNIELDRKQKLLEEPDATERLKNLARYLADEMEV
                     MKLGRKLQADVKKEMDREQREYYLRQQLKAIRRELGEDEDRTVETEELREKIDGAQLT
                     PQAREVAVKELDRLQKMSPGAAEYTVSKTYIDWILDLPWGISTDDNLDMKEAEKVLDE
                     DHYDLERVKERILEFLAVKKLKRSMKGSIICFAGPPGVGKTSLGKSIARSMGRKFVRI
                     SLGGMRDEAEIRGHRRTYIGALPGRIIQGMRTAGSNNPVFMLDEIDKVGMDFRGDPSS
                     ALLEVLDPEQNNSFQDNYLEVPFDLSKVLFIATANMVDSIPPALRDRMEIIQLPGYTQ
                     LEKLRIAQQFLVPKQLENHGL"
BASE COUNT          700 a         1247 c         1217 g          618 t
ORIGIN      
        1 gatctcgccc tggatctacc gggtcggcac cttcgtcgac ttcggtcgac tcgcgacacc
       61 ggttcgcccg ggccagctcg acaccgcgga gcggctgcgg ggcgacacca cgtttcgctt
      121 cgaggccgtt ccgtacgccg ccttcgccta cggagtgggc ttcacgctgg gcccgtcgct
      181 gcgcgagctc cacgagacca cgtcgctcgc gggggtattc gcgctccacg gcgccgtgat
      241 cgtgtgggtg ggcgccctct ttggcgcgct gctcgtgcgc ggcgtggcgc gcggcgcgcg
      301 cgatcacgga ccttggtcgg tggtcgagcc cttgctctac gtcggcatcc cgttgctcgc
      361 cacgctggcg ctcaattggc agaacgcgaa agcgttcaac gtgcgttacg tgctggtggg
      421 gctaccgatg tacctagccc tggtcgcact cggcgtcgcc tcgctcccgc gcgcgcgcga
      481 agcgctggcc ggcaccctgg tggccgcgac ctgcgcggtg tcgttgtgga attactacac
      541 cgacccgcgc tacgccaagg aggacgtacg ctcggcggtg cgcgccatcg aagcgcgcat
      601 cgcacccgga gagtgcatcc tcgcgccgac ggtctggcag atcgtcgagg tgtacgcgac
      661 caccgacgca cccgtgctca acgtctacgg ctcgcccgaa tcgacgatgc aagatcaact
      721 cgggcgtctc gtcgtgcaat gcgattcgtt ttggtacgtg cgcgcgcgca cctgggtcga
      781 tgacccgcaa ggccgcgttc ttcgagaaat cgatgcgcgc tacgtacgtg gtgagacatg
      841 ggagttcccc ggagtttccg cgagtcactt cgcccgcaga cgctagccgc ttcctccgcg
      901 aagttgggac actttgtgca ttaggtaaca aggccggcgg acgttcggcc ccgaccgggg
      961 cattcgccgg ccgcgcgacc tctcgcgcgc aattcccgga catcgttgga aagcaggagg
     1021 catccatgcg tccccgccac ccctatagct caatcgccct cgcgatcgcg gtcgctctgt
     1081 gcgcctcgtg cgcggccagc ccgccgcggc gcgacgcgac cccggagatc caagcgatcc
     1141 gcgacgacta tctgcgcgcg aaccccgacg tcgtctacaa ggacgccatc gcgcgcggcg
     1201 aggtcgtggt cggcatgggg taccaggacg tgctggcggc gtggggccgt cccacggcgc
     1261 gcgtcgtcca gcaaggcacc gacgacgcgg agcgctggac ctacgtactc gtcagcgaca
     1321 acggcaccga ctgggtccgc tacgatttct tcttcgcgaa gtggtcggtg gtgcagtggg
     1381 agaccacgcg cagtgtggcc tccggtttcg cccccgcgcg cgacgacgcg cgcggcgtgt
     1441 cgacgcgcgt gcctcccatg cccgcgccgg ccgcgctggg cgaaggggtc cgcaagggtg
     1501 gtgcggggtc gatgattcga tagcgagcgc gtcgtgcgcg cggtcgtttt cgccgtttcg
     1561 gccccgcgga aatccttccg cggggccctc gcttttcttg acaggggaaa ccccgcgccg
     1621 gtacagtcct ttccgccgcc gtaatatgcg gcgatgccaa cggttagcgc cattcgttgc
     1681 gaggagagta ggttatgaat acgaggttgc cgcacgcccc cgcgtggccg ctgtctcgcg
     1741 agctcgacat gagcgagttc gacaacattc gcgagctgct cgagatgctg atgtccgagc
     1801 acacgccggc caagcacaaa atcaagatga agacgagctt cggctgggaa ccggccatga
     1861 acgtcttcga gaccgacggc gagttcgtgg tcatcatgga catcgccggc atggatccca
     1921 aggagatcac ggtcttcacc gacggcaaca tcctgcgcat tagcggcgtt cgcaaggacc
     1981 tgatcccgcc cggcaagaag cagttccatg cgctggagat tcaggtcggt cctttccagc
     2041 gcctgatcgg gatccccgtc ccggtcgatc gcgacagcgt gaccacgcac tacgcgaacg
     2101 gtctgctgga gattcgtttg aagaagcgcg gcgatcaccc cggcaagcgt cgggtcgaga
     2161 tcgagtagcg cgctcccaac cccggagtaa tcggtgaagt cgcgaagcac cctcgagttc
     2221 gaagagccgg gcgtcagcac ggggagtatc ccgagcgagc tcgcgatact gcccatcaac
     2281 gatgcggtgg tattcccgtt gatgatggtg ccgctcatcc tgcgcgaccc caatctcctg
     2341 cgcctggccg acgaggcgct cgccgagcac aagatcatcg gcgccttcac gcagaagaac
     2401 gccgagaccg agcacccggg cccggacgat atctaccgcg tcggcaccgc catccacatc
     2461 cagaagatga tccgtttccc aaacggcgag atgcgcttgc tcgcgcaggg aatcgcgcgc
     2521 atccagctca ccgagatcac gcaggtggtg ccgtacttga aagggaaggt gcgcccgctg
     2581 gacgagatcg agagcgacac cgacctccac aaggcctacg tgcagaacgt cgtgcgcacc
     2641 ttctcgaaga tgatcgacga gtcggacacc ctggccgacg agctcaagat catcatcaac
     2701 aacatcaagg aaggcgggcg gctggccgac gtgatcgcca ccaatctcaa tatcgagctc
     2761 gatcgcaagc agaagttgct cgaagagccc gacgccaccg agcgcctgaa gaatctcgcg
     2821 cgctacctcg ccgacgagat ggaggtcatg aagctcgggc gcaagctgca agcggacgtc
     2881 aagaaggaga tggaccgcga gcaacgcgag tactacctgc gccagcagct caaggccatc
     2941 cgccgcgagc tgggggaaga cgaagaccgc accgtcgaga ccgaggaact gcgcgaaaag
     3001 atcgacggtg cccagctgac accgcaggcg cgcgaagtgg ccgtgaaaga gctcgaccgg
     3061 ctccagaaga tgtcccccgg cgccgcggag tacacggtgt cgaagaccta catcgactgg
     3121 attctcgatc tgccgtgggg aatctccacc gacgacaacc tcgatatgaa agaggcggag
     3181 aaggtcctcg acgaggacca ctacgacctg gagcgcgtca aggaacgcat cctcgagttc
     3241 ctggcggtca agaagttgaa gcgatccatg aagggatcga tcatttgctt tgcgggtccg
     3301 ccgggagtgg gcaagacctc gctcggcaag tcgatcgcgc gctcgatggg gcgcaagttc
     3361 gtgcgcatct cgctcggcgg catgcgcgac gaggcggaga ttcgcgggca ccggcgcacc
     3421 tacatcggcg cccttccggg gcgcatcatc caaggcatgc gcaccgcggg cagcaacaac
     3481 ccggtgttca tgctggacga gatcgacaag gtggggatgg acttccgcgg cgacccgtca
     3541 tcggcgctgt tggaggtcct cgaccccgag cagaacaaca gcttccagga caactacctg
     3601 gaggtgccgt tcgacctgag caaggtcctg ttcatcgcga ccgcaaacat ggtcgattcc
     3661 atcccgcccg ccctgcggga ccggatggag atcattcagc tgcccggcta cacccagctc
     3721 gagaagctgc ggatcgccca gcagttcctc gtccccaagc agctcgagaa ccacggcctg
     3781 ag
//
LOCUS       DASPGK010000038         5767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_829701, whole genome shotgun sequence.
ACCESSION   DASPGK010000038 DASPGK010000000
VERSION     DASPGK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829701"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VEC56_00975"
     CDS             complement(<1..734)
                     /locus_tag="VEC56_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXV12753.1"
                     /translation="MAKITMIGTGYVGLVSGACLADFGHDVTCVDIDKDRIAALKRGE
                     IPIYEPGLKDVVVKNADGERLQFTTDLAPAVREAEVVFIAVGTPPQEDGGSDLSFVFG
                     AARDIAKHLEGYTVVVQKSTAPVGTAREIGRAIAAAKPKDAECDIVANPEFLREGSAV
                     NDFMRPDRVVVGAETERSRQLMKQVYRPLYLLETPIVWTSLESAEMIKYASNAYLAAK
                     ISFINEMARICELLGANVDDVARGMGL"
     gene            complement(727..1683)
                     /locus_tag="VEC56_00980"
     CDS             complement(727..1683)
                     /locus_tag="VEC56_00980"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXV12754.1"
                     /translation="MERALVTGCNGFVGRALVQRLSGAGYDVWGVDRTEADPAFKGKR
                     YLRGDLTDAEFVASVLDDASPACIVHLAAQSSVAKSFEAPASTLHDGTAPALHILEHV
                     RRSKTPVRILLIGSADEYGSVNPAQIPVREDTPTNPESPYALAKSIQNHYGRMYAKLY
                     RVDAMMTRSFNHTGPGQSDVFVLSSFAKQVAEIRRGLREAEIEVGNLDVRRDFMDVRD
                     VCDAYVVLLKKGRMGETYNVCSGQSFKVGDLLRELCSLAGAHVAIRVDVARLRPVDTP
                     ELRGDPSKMSQHTGWTTRHAIKDTLKSLLDYWDGVLEGKTHG"
     gene            complement(1684..2634)
                     /locus_tag="VEC56_00985"
     CDS             complement(1684..2634)
                     /locus_tag="VEC56_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXV12755.1"
                     /translation="MRVLITGITGFAGSHLADFCLARGGLDVHGIVRWRSRMENVEHL
                     AGKVLLHECDLRDASSTRDVVEHIRPDYIFHLAAQSFVPTSWKAPAESLTTNVIGQLN
                     IFEAVRKIGLKCRIQLACSSEEYGMVYENEVPIKETNPLRPLSPYGVSKVGQDLLGYQ
                     YYMSYQMDIVRTRGFNHEGPRRAPVFVMSDFAKQIVDIERGAKAPVLRVGNLDAKRDF
                     TDVRDMVRAYWLALEKGKPGEVYNICQEKCWSIRDMLDQLLTMTTAKIKVETDQDRLR
                     PSDVMLLLGDCSKFRADTGWKPTIPFAQTLRDTLDYWRGR"
     gene            2739..4073
                     /locus_tag="VEC56_00990"
     CDS             2739..4073
                     /locus_tag="VEC56_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV12756.1"
                     /translation="MRTVSRFVAAAALLPALAFSPEPARANFTAASLTPYDGFFVTRV
                     DVEGCKVTKEYIVHREIRIQPGDTFRVADAQADLTRLENLGIFSSGEIVAAATDSSVA
                     LTYRVREMPWIVPFPRVRYTEQDGWSFGAGVSSVNMHGRATRLSGSFLLGGLDSWSVR
                     YRYPWITGNHISFDFFASDFEREDDLNEFREHSFELTPWFGSYIKDHGRVGLTVSYFQ
                     MKSDRDGITLSGDRRDRFLRVGGRIGHDSRDNWRNPCRGWLHDFLALYNDGGTFGEPG
                     QWWLAEADVRRYQPLDDKLALIAGALVSYQDGQVGADIPGYMQYRMGGANSIRGYDID
                     VLGQEVYGRNQAIVTAEVHRVLSPMRERFIGKWSYSAGVEAAAFVDWGIAWSGSDEFD
                     DKRARTGFGLGLRWLLPAVFEVRTDVAMGEDGKAYFHLGVGEKLNAQRARLR"
     gene            4087..5442
                     /locus_tag="VEC56_00995"
     CDS             4087..5442
                     /locus_tag="VEC56_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12757.1"
                     /translation="MKKRFALMMCVVCLWSRASVAQDTNYWTLQYGTRGELLGGVVVG
                     SALDMSATFYNPGAIAYVENPSFILTASVFGMQTIKLIDQDPEQEGLTTTSFGPLPSM
                     FAGILPMKWFGGRTAYSFLTRQQLDFRITAREGVVVGRDEPGDTLSVGGEVIMTQNMG
                     ENWGGFTWAKKLGERASLGATLYGVYRGQYTHQTQTVEAIGSSGFGASAIVENELDYY
                     DVRTLGKVGVYVDLGRGSVGMSFTTPSASLFGTGSIAQNRSLIGDTDGDGTDDSRAEV
                     AFGEDLDTEYKSPMSVAVGGAYQFKRFKWHATVEYFAAVEQYTLVEAPAPAAGPGVTG
                     IDIEYQGASTSVVNWGLGFEQGFGDDNWFYVSYLTDQSAYQAVDERRSVVATWDINHL
                     NGGVALTFGEAELTLGGGFAWGQNKAVSDVNPGTSVLPQTVVPGGVGYTRLKFIVGFA
                     L"
     gene            complement(5452..>5767)
                     /locus_tag="VEC56_01000"
     CDS             complement(5452..>5767)
                     /locus_tag="VEC56_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12758.1"
                     /translation="ESKDVMGILVAMSLAGLLALAAVAAAVFIGPERIKRNAVLRGAV
                     VLGLLAGVAVAGYALWAQRSSIEWSTGANAGALFVIVPGLLVLGGPIVVALRYLPALL
                     QR"
BASE COUNT          941 a         1896 c         1902 g         1028 t
ORIGIN      
        1 agtcccatcc cgcgcgcgac gtcgtccacg ttggcgccca gcagctcgca gatgcgcgcc
       61 atttcgttga tgaatgagat cttcgccgcg aggtacgcgt tggacgcgta cttgatcatc
      121 tcggccgact cgagcgacgt ccacacgatg ggcgtctcga gcaagtacag cgggcgatac
      181 acctgcttca tgagctggcg cgagcgctcg gtctcggcac ccaccacgac gcggtcgggg
      241 cgcatgaagt cgttcaccgc ggagccctcg cgcagaaact ccggattggc gacgatgtcg
      301 cactcggcgt ccttgggctt ggcggccgcg atcgcgcgtc cgatctcgcg cgcggtgccc
      361 acgggtgcgg tgctcttctg caccaccacg gtgtagccct ccaggtgctt cgcgatatcg
      421 cgggccgcgc cgaacacgaa gctcaagtcg ctgccgccat cctcttgcgg cggcgtgccc
      481 acggcgatga acacgacctc ggcctcgcgc accgccggtg ccaagtcggt cgtgaactgg
      541 aggcgctcgc cgtcggcgtt cttcaccacg acgtctttca atcccggctc gtagatgggg
      601 atctcgccgc gcttgagcgc cgcgatgcgg tccttgtcga tgtcgacgca ggtcacgtcg
      661 tggccgaagt cggcgaggca cgccccgctc accagcccga cgtacccggt tccgatcatg
      721 gtgatcttag ccatgggtct ttccctccag cactccgtcc cagtaatcga gcagggattt
      781 caacgtgtcc tttatcgcgt gcctggtggt ccagcccgtg tgctggctca tcttcgacgg
      841 gtcgccgcgc agctccggcg tgtcgacggg acgcaacctc gcgacgtcga cgcggatcgc
      901 gacgtgggcg ccggccaagg aacacagctc gcgcaagagg tcgcccacct tgaacgattg
      961 ccccgagcac acgttgtagg tctcgcccat gcgccctttc ttcaggagca cgacgtaggc
     1021 gtcgcatacg tcgcgcacgt ccatgaagtc gcggcgcacg tcgaggttgc ccacttcgat
     1081 ctcggcttcg cgcagcccgc ggcggatctc cgccacctgc ttggcaaaac tcgacaacac
     1141 gaatacgtcg ctttgcccgg ggccggtgtg attgaagctg cgcgtcatca tggcgtcgac
     1201 gcggtagagc ttggcgtaca tgcggccgta gtggttctgg atcgactttg cgagcgcgta
     1261 cgggctctcg gggttggtgg gggtatcttc gcgcaccggt atctgcgcgg ggttgaccga
     1321 gccgtactcg tcggccgagc cgatcaggag aatgcgtacg ggggtcttgc tgcggcgcac
     1381 gtgctccagg atgtgcagcg ccggcgcggt gccatcgtgc agcgtggacg cgggtgcttc
     1441 gaacgacttg gccacactgg actgcgccgc gaggtgcacg atgcacgccg gcgacgcgtc
     1501 gtcgaggaca ctcgcgacga actcggcgtc ggtcaggtcg ccgcgcaggt aacgcttgcc
     1561 cttgaacgcg gggtcggcct cggtgcgatc gacaccccac acgtcgtacc ccgcgcccga
     1621 gagccgctgc accagggcgc ggccgacgaa tccgttacag cccgtgacga gtgcgcgttc
     1681 cacttatctt ccgcgccagt agtcgagcgt gtcgcgcagc gtttgcgcga acggaatggt
     1741 cggtttccag cccgtgtccg cgcggaactt cgagcagtcg cccagcagca gcatcacgtc
     1801 cgaggggcgc aggcggtcct ggtcggtctc gaccttgatc ttggcggtcg tcatggtgag
     1861 cagctgatcc agcatgtcgc ggatcgacca gcacttctcc tggcagatgt tgtacacctc
     1921 gcccggcttc cccttctcca gcgcgagcca gtacgcgcgc accatgtcgc gcacgtcggt
     1981 gaaatcgcgc ttggcgtcca ggttgccgac gcgcagcacg ggcgccttcg cgccgcgctc
     2041 gatgtcgacg atctgtttgg cgaagtcgct catcacgaac acgggcgcgc ggcgcggacc
     2101 ctcgtggttg aacccgcgcg tgcgcacgat gtccatttga taactcatgt agtactgata
     2161 ccccagcaag tcctgcccca ccttgctgac accgtatgga gaaagcggcc gcagcggatt
     2221 ggtctccttg atggggacct cgttctcgta caccatgccg tactcttcgc tcgagcacgc
     2281 gagctggatg cggcacttca agccgatctt gcgcaccgct tcgaagatgt tgagctggcc
     2341 gatgacgttg gtggtgaggc tctccgcggg cgccttccac gaggtcggca cgaagctctg
     2401 cgcggccaag tgaaaaatgt agtcgggtcg aatgtgctcg accacgtcgc gcgtgctgga
     2461 ggcgtcgcgc aggtcgcatt cgtggagtaa caccttgccc gcgaggtgct cgacgttctc
     2521 catgcggctg cgccagcgca cgatgccgtg gacgtcgagg ccgccgcgcg cgaggcagaa
     2581 gtcggccagg tggctcccgg cgaagccggt gattccggtg atcaagacgc gcacgaacag
     2641 gggtcctttc gaagggtttc gagacgccta ggctacgcga aaagtgcatt ggggtcaaag
     2701 ggtttggaag tcggccgggg ccgtgctaac ctgcgccgat gcgcacggtt tctcgattcg
     2761 tggcggccgc cgcgctcttg ccggcgctgg cgttctcgcc cgaacccgcc cgcgccaatt
     2821 tcaccgccgc ctcacttacg ccctacgatg gattcttcgt cacgcgcgtc gacgtcgagg
     2881 gctgcaaggt gaccaaggag tacatcgtgc accgcgagat ccgcatccag cccggcgaca
     2941 ccttccgcgt cgccgacgcc caggccgacc tcacgcggct cgagaacctc ggcatcttca
     3001 gctcgggcga gatcgtggcg gcggccaccg acagctcggt ggcgctcacc taccgtgtac
     3061 gcgagatgcc gtggatcgtt cccttcccgc gcgttcgtta caccgagcag gacggctggt
     3121 cgttcggcgc cggggtgtcg tcggtcaaca tgcacgggcg cgccacgcgg ctctcggggt
     3181 cgttcctctt gggcggtctc gactcgtggt cggttcgtta ccgctacccg tggatcaccg
     3241 gcaaccacat ctcgttcgat ttcttcgcct ccgacttcga gcgcgaggac gatctcaacg
     3301 agttccgcga gcattcgttc gagctgacac cgtggttcgg gagctacatc aaagaccacg
     3361 gccgcgtcgg cctcaccgtt tcgtactttc aaatgaagag cgaccgcgac ggcatcacgc
     3421 tctcgggcga tcgccgcgat cggttcttgc gcgtgggcgg gcgcatcggc cacgattcgc
     3481 gcgacaactg gcgcaacccg tgccgcgggt ggctgcacga cttcctcgct ttgtacaacg
     3541 acggcggcac cttcggcgag ccgggccagt ggtggctcgc ggaggcggac gtgcgccgct
     3601 accagccgct cgatgacaag ctcgcgctca tcgcgggcgc actggtgtcg taccaagacg
     3661 gccaggtcgg tgccgacatc cccggctaca tgcagtaccg catgggcggg gccaactcga
     3721 ttcgcggcta cgatatcgac gtcttgggcc aggaggtgta cggtcgcaac caggccatcg
     3781 tcaccgccga ggtccaccgt gtgctctcgc ccatgcgcga gcgcttcatt ggcaagtggt
     3841 cgtacagcgc gggggtcgaa gcggccgcct tcgtcgactg gggcatcgcg tggagcggca
     3901 gcgacgagtt cgacgacaag cgcgcgcgca ccggcttcgg gctggggttg cgctggcttt
     3961 tgcccgcggt gttcgaggtc cgcaccgacg tcgcgatggg cgaggacggc aaggcttatt
     4021 ttcatctcgg tgtgggtgag aagctcaacg cgcagcgagc gcggcttcgt taggaggatg
     4081 atcggcgtga agaaacgatt cgctctgatg atgtgtgtgg tatgcctctg gtcgcgcgcg
     4141 tcggtcgcgc aggacacgaa ctactggacc ctccagtacg ggacgcgcgg cgagctcttg
     4201 ggcggcgtgg tggtcggcag tgccctcgac atgagtgcca ccttctacaa cccgggtgcg
     4261 atcgcgtacg tcgagaaccc ctcgttcatc ttgaccgcga gtgtcttcgg catgcagacg
     4321 atcaagctca tcgaccagga ccccgagcaa gaagggctga ccacgaccag cttcggcccg
     4381 ctgccttcga tgttcgcggg catccttccc atgaagtggt tcggcggcag gacggcgtac
     4441 tcgttcctca cgcgccagca gctcgatttc cgtatcaccg cgcgcgaagg cgtggtggtg
     4501 gggcgcgacg agcccggcga cacgctttca gtcggcggcg aagtcataat gacccagaac
     4561 atgggcgaga actggggcgg cttcacctgg gcgaagaagc ttggcgagcg cgcgtcgctg
     4621 ggcgcaaccc tctacggtgt gtaccgcggg cagtacacgc accagaccca gaccgtcgaa
     4681 gccattggct cgagcggctt cggcgcatcc gccatcgtcg agaacgagct ggattactac
     4741 gacgtgcgca cgctcgggaa agtgggcgtg tacgtcgatc tggggcgcgg gtcggtcggc
     4801 atgtcgttca cgactcccag cgcgagcttg ttcggcacgg gcagcatcgc acagaaccgg
     4861 tcgctgatcg gcgacaccga cggcgacggc accgacgact cgcgcgccga ggtcgccttc
     4921 ggcgaggacc tcgacaccga atacaagtcc ccgatgtcgg tcgccgtggg cggcgcgtat
     4981 caattcaagc ggttcaagtg gcacgcgacc gtcgagtact tcgccgccgt cgaacaatat
     5041 accctggtcg aagcccccgc gcccgccgcc ggtcccggtg tgaccggcat cgacatcgag
     5101 tatcagggcg catcgacgtc ggtggtcaac tggggactcg gcttcgagca gggtttcggc
     5161 gacgacaact ggttctacgt gagctacctc accgaccagt ccgcgtacca ggccgtcgac
     5221 gagcggcgca gcgtcgttgc gacgtgggac atcaatcacc tcaacggcgg cgtggcactc
     5281 acgttcggag aagcggagct gacactcggc ggcgggtttg cgtgggggca gaacaaggcc
     5341 gtgagcgacg taaacccggg cacgtcggtg ctgccacaga cggtggtgcc gggtggcgtg
     5401 ggctacacgc gcttgaagtt catcgtcggc ttcgcgctgt aggtcgaaga atcagcgctg
     5461 caagagcgcc ggcaggtagc gcagtgccac tacgatcggg ccgccgagca cgagcaatcc
     5521 cggcacgatg acgaacagcg ctcccgcgtt ggcgcccgtc gaccattcga tgctcgagcg
     5581 ctgcgcccac agcgcatagc ccgccaccgc gacccccgcc agcaagccga ggacgacggc
     5641 accgcgcagc acggcgtttc gcttgatgcg ctcggggccg atgaacaccg ccgccgcgac
     5701 ggccgcgagc gcgagcaacc ccgccagtga cattgcgacc aggataccca tcacgtcctt
     5761 gctctcg
//
LOCUS       DASPGK010000039         4127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1649653, whole genome shotgun sequence.
ACCESSION   DASPGK010000039 DASPGK010000000
VERSION     DASPGK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649653"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VEC56_01005"
     CDS             <1..1367
                     /locus_tag="VEC56_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXV12759.1"
                     /translation="GRKGKTLWTDGEVGDKVRVTASIDEESEALAVIDLVKREVAAGT
                     SLKQIAVLYRTNAQSRALEDVFVQSSLPYQIIGSVRFYERAEVRDILAYCKAIVNPAD
                     SINLKRILNVPRRGIGKTTIDQIEALAADRGTSLVDAMRAAASTLGAAAASRVHAFLV
                     LFDELRRLAESQVAPHVVDAILNATEYHKHLEENYPDAEARIENVDELLSKAHGFAEG
                     AETKSLSAFLEEVALVADVDTLDLESGQLTLMTIHNAKGLEFEAVIVTGMEEGLFPHY
                     NSIDDPTAVEEERRLFYVGMTRARRRLYLTFAGMRRRMGMMEGGMPSRFLNEIPERTL
                     EAPVEPLVSPTLYGGAWSPRRDYARSVRDEYAQPEPDEAYSQETVVSPVVSGVRVSSN
                     APSSAGALDHAYSIGMRIQHERFGKGIVRKVEGKGEQTRVTVIFDAGGERKFLAHYAP
                     MRPL"
     gene            1382..1690
                     /gene="gatC"
                     /locus_tag="VEC56_01010"
     CDS             1382..1690
                     /gene="gatC"
                     /locus_tag="VEC56_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXV12760.1"
                     /translation="MSKIDRDVVRHLERLARIDLSPDEVEMITAQLGRIVGFVEKLQA
                     ANVENIEPTRAMSHEAADHVREDVVTPSLDRKVVLGQAPDHTPDYFRVPRVIEKGDEE
                     "
     gene            1687..3162
                     /gene="gatA"
                     /locus_tag="VEC56_01015"
     CDS             1687..3162
                     /gene="gatA"
                     /locus_tag="VEC56_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXV12761.1"
                     /translation="MSLYELSIAELRRRIASGEVTPLEATDAALSQIDARDRDVHAFL
                     DVHGDRARERAKALATTGVARTLPLAGVPIAIKDNMCVEGTPTTCGSKILGRYQPPYT
                     ATAVARLEDAGAIVVGKTNLDEFAMGSSTENSAFGPTRNPWDLSRAPGGSSGGSAAAV
                     SAGMAAAALGSDTGGSIRQPAALCGVVGMKPTYGRVSRYGLVAFASSLDQIGPFARSV
                     EDAALILNALCGKDSMDASSAGVAVPDFTNDLAKGLKGLRIGIPWEFLGPHVDPAVRK
                     SFDTTIDAAKREGADIVDVALPNADFGLAAYYIVANAEASANLARFDGIRYGHRSPRA
                     QSLYETYAKTREEGFGREVKRRVLLGTYVLSAGYYDAYYRRAQQVRSLIIKDFATAFE
                     RCDVVALPTSPTPAFKLGDKTDDPILMYLNDVFTIPVNLAGLPGISVPCGMSPERLPI
                     GLQLIGRAFDETKLLRAAAGMERVADFPTGAVAPVSKGARS"
     gene            3159..>4127
                     /gene="gatB"
                     /locus_tag="VEC56_01020"
     CDS             3159..>4127
                     /gene="gatB"
                     /locus_tag="VEC56_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXV12762.1"
                     /translation="MSAYEAVIGLEVHAQLSTNSKMFCGCSAAFGGAPNTHVCPTCLG
                     LPGALPVVNARAVEFAVRLGLATECTIANESIFARKNYFYPDCPKNYQISQFDTPLCE
                     HGRLAIENDAKHVRIRRIHLEEDAGKLVHMEGKDHSLVDMNRSGVPLVEIVSEPDIRT
                     PQEASEYLQKLRSLVRYLGICDGNMEEGSLRCDVNLSVRKHGSQELGVKTEVKNLNSF
                     KQVEQAIEFELDRQTKLLESGGRVTQDTLLWDPAAKRATVMRSKEEAHDYRYFPEPDL
                     RVLRVKDELIARVRSELPELPDAKQKRFVSQYGIPAYDAHVLTGSGD"
BASE COUNT          737 a         1371 c         1350 g          669 t
ORIGIN      
        1 tgggccgcaa gggcaagacc ctgtggaccg acggcgaggt gggcgataag gtgcgggtga
       61 ccgcgtccat cgacgaagag tccgaggcgc tggccgtgat cgacctggtc aagcgcgaag
      121 tggccgcggg cacgagcctc aaacagatcg cggtcttgta tcgcaccaac gcgcagtcgc
      181 gcgcgctcga ggacgtcttc gtccagagtt cgttgccgta tcagatcatc ggctcggtgc
      241 gcttctacga gcgcgccgag gtgcgcgaca tcctcgcgta ctgcaaggcc atcgtcaacc
      301 cggccgactc gataaatctc aaacgaatcc tcaatgtgcc tcgccgtgga atcggtaaga
      361 cgacgatcga tcaaattgaa gcgctcgccg ctgatcgtgg aacttccttg gtcgatgcga
      421 tgcgagcagc ggcgagtacg ttgggtgcgg ccgccgcctc ccgcgtgcac gccttcctgg
      481 tcttgttcga cgagctgcgg cgcctggcgg agagccaggt ggcgccgcac gtggtcgacg
      541 ccattttgaa cgcgaccgag tatcacaagc acctcgagga gaattatccc gatgccgagg
      601 cgcgcatcga gaacgtcgac gaactcctgt cgaaggccca cgggttcgcg gagggggccg
      661 agaccaagtc gttgagcgcg tttctcgaag aggtggcgct cgtcgccgat gtcgacacgc
      721 tcgatctcga gagcggtcag ctgacgctca tgaccatcca caacgcgaag ggcctcgagt
      781 tcgaggcggt gatcgtcacc gggatggaag agggattgtt cccgcactac aactcgatcg
      841 acgacccgac cgcggtcgaa gaggaacggc ggctgttcta cgtcggcatg acgcgcgcgc
      901 gccggcgtct ctatttgacg ttcgcgggca tgcgccgccg catggggatg atggaaggcg
      961 gcatgccgtc gcgctttttg aacgagatcc cggagcgcac gttggaagcg ccggtcgagc
     1021 cgctggtttc tcccacgctc tatggaggcg cctggtcgcc gcgccgcgat tacgcgcgct
     1081 cggtgcgcga cgagtacgca caacccgagc ccgacgaggc gtactcgcag gagacggtcg
     1141 tttctcccgt cgtttcgggc gtgcgggtct cgtcgaacgc gccctcgagc gcgggtgcgc
     1201 tcgaccatgc ctactcgatc gggatgcgca tccagcacga gcgcttcggg aagggaatcg
     1261 tgcgcaaggt ggaaggcaag ggcgagcaga cgcgcgtcac cgtgatcttc gatgccggcg
     1321 gcgagcgcaa gttcctcgcg cactacgcgc ccatgcggcc gttataactc cggagggcca
     1381 cgtgtccaag atcgaccgcg acgtcgtccg ccacctcgag cgcctcgccc gtatcgacct
     1441 ctcgcccgac gaagtcgaga tgatcaccgc gcagctcggg cgcatcgtcg gcttcgtgga
     1501 gaaattgcag gccgcgaacg tggagaacat cgagcctacg cgcgcgatgt cgcacgaggc
     1561 cgccgaccac gtgcgcgagg acgtggtgac gccgtcgctc gatcgcaagg tcgtcctcgg
     1621 gcaggcgccg gaccataccc ccgactactt ccgcgtcccg cgcgtcatcg aaaagggcga
     1681 cgaggaatga gcctctacga gctctcgatt gccgagctcc gccggcgaat cgcctccggc
     1741 gaggtaaccc cgctcgaggc caccgacgcg gccctctccc agatcgacgc gcgcgaccgc
     1801 gacgtgcacg ccttcttgga cgtgcacggg gatcgcgcgc gcgagcgcgc gaaggcgctc
     1861 gccacgaccg gcgtggcccg aacgctgccg ctcgccggcg tccccattgc gatcaaggac
     1921 aacatgtgcg tcgaagggac cccgaccacg tgcggatcca agattctcgg aagatatcag
     1981 ccgccctaca ccgcgaccgc ggtcgcgcgc ctcgaggacg cgggcgcgat cgtggtcggc
     2041 aagaccaacc tggacgaatt cgcgatgggc tcgtctacgg agaattcggc gtttggcccg
     2101 acgcgcaacc cgtgggatct ctcgcgcgcg ccgggcggat caagcggcgg gtcggcggcg
     2161 gcggtgtcgg cggggatggc cgcggccgcg ctgggcagcg acaccggcgg ttccattcgc
     2221 cagccggctg cgctgtgcgg tgtggtcggc atgaagccca cctacgggcg ggtgtcgcgc
     2281 tacggcctgg tcgcgttcgc gagctcgctc gaccagatcg gccccttcgc gcgttcggtc
     2341 gaagacgccg cgttgatctt gaacgcgctt tgcggcaaag actcgatgga cgcgagcagc
     2401 gcgggcgtcg ccgtacccga tttcaccaat gacctcgcca agggattgaa gggtttgcgt
     2461 attggcatcc cctgggagtt cttgggaccg cacgtcgacc cagccgttcg caagtccttc
     2521 gacacgacca tcgacgccgc caagcgcgaa ggcgccgaca tcgtcgacgt ggccttgccc
     2581 aacgccgact tcgggctcgc ggcctactac atcgtggcca acgcggaggc gagtgccaac
     2641 ctcgcgcgct tcgacgggat tcgctacggc caccgctcgc cgcgcgcgca gtcgctctac
     2701 gagacctacg ccaagacgcg cgaggaaggc ttcgggcgcg aggtgaagcg ccgcgtgctg
     2761 ctcggcacct acgtcttgag cgcgggctac tacgacgcct actaccgccg cgcgcagcag
     2821 gtgcgctcgc tcattatcaa ggatttcgcg acggccttcg agcgctgcga cgtggtggcg
     2881 ctgcccacct cgcccacacc cgcgttcaag ctgggcgaca agaccgacga tcccattctc
     2941 atgtatctca atgacgtgtt caccattccc gtgaacctcg cggggttgcc gggtatttcc
     3001 gtaccgtgcg gaatgtctcc ggaaagactt cccatcggac tccagctcat tggccgtgcg
     3061 ttcgatgaaa cgaaactgct ccgtgccgcc gcgggcatgg agcgcgtcgc tgactttccg
     3121 acgggcgcgg tcgcgcccgt ttcgaaggga gcgcggtcgt gagcgcctac gaagccgtca
     3181 tcggactcga agtccacgcg cagctctcca cgaacagcaa gatgttctgc ggctgctcgg
     3241 ccgccttcgg cggggcaccc aacacccacg tctgtccgac ctgcctgggt ctcccggggg
     3301 cgctgcccgt cgtcaacgcg cgcgcggtcg aattcgcggt acgcttgggc ctcgcgaccg
     3361 agtgcaccat cgccaacgag agtatcttcg cgcgcaagaa ctacttctat cccgattgcc
     3421 cgaagaacta ccagatttcg cagttcgaca cgcccctgtg cgagcacggg cgcctggcga
     3481 tcgagaacga cgccaagcac gtccgcattc gccgcattca cttggaggaa gacgccggca
     3541 agctggtcca catggaaggg aaggaccaca gcctggtcga catgaaccgc agcggcgtgc
     3601 cgctggtcga gatcgtgagc gagcccgata ttcgcacgcc ccaagaggcg agcgagtacc
     3661 tccagaagct acgctcgctc gtgcgctacc tcggcatctg cgacggcaac atggaagagg
     3721 ggagcctgcg ctgcgacgtg aatttatccg tgcgtaagca cggatcccag gagctcggcg
     3781 tcaagaccga ggtgaaaaac ctgaactcct tcaaacaggt cgagcaggca atagagttcg
     3841 agctggatcg tcagacgaag ctgctggagt ccggcgggcg cgtgacccag gacaccctgc
     3901 tctgggaccc ggccgccaag cgcgcgaccg tcatgcgcag caaggaagag gcgcacgatt
     3961 accggtactt cccggagccc gacctgcgcg tgctgcgcgt gaaggatgaa ctgatcgcgc
     4021 gcgtgcgctc cgaactcccc gaactcccgg acgccaagca gaagcgcttc gtgtcccagt
     4081 acggcatccc cgcctacgat gcccacgtgc tgaccggctc gggcgac
//
LOCUS       DASPGK010000040         9227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_126863, whole genome shotgun sequence.
ACCESSION   DASPGK010000040 DASPGK010000000
VERSION     DASPGK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9227
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126863"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..2158)
                     /locus_tag="VEC56_01025"
     CDS             complement(329..2158)
                     /locus_tag="VEC56_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HXV12763.1"
                     /translation="MTRALRIVSMVALVCAAPVAHAQVSVSPQPTSGSVNQTVLVDVV
                     LTNPGAAPVDAFGFRLSYPEALLAYQSVSVAGTLTDGWIAVSGAETTPGVVQVGGFHT
                     TPTTAGGVLVRVAFLVETNLLTSGAIALSNFVDDITAATTTPATFQVVVAPGGAGLLG
                     EYYNNIDFTGTLLRRVDATVNFDWALGSPDPSMGTNDFSIRWTGYVDPAFTQTYTFYT
                     ITDDGVRLWVNDQLIIDYWIPQAAIERSGSIALTAGVPASIRMEMFEEAGEAVAQLSW
                     SSPSQGKQIIPSNRLIASLCAQGIGDVDATGLLDASDVACAFDMFLSGQAVLPGCDSP
                     STTCELSAADVNCSGAVTPADARAIEIRAAASLAPAPCFAPPDPVPSPPHELGLVQYI
                     VDDGGTPRLQVLLVVEDASDLDAFGARLSFPASELVFNRVEPGYLTAGWHSIDGRSVA
                     VGQIVLGGFDPTMTAPPGTADVCRIFFDFVGSPATVTGLVLSNFVDDFTGATMGAVTD
                     VEPQVGIHSLHPNYPNPFNPSTHVRYDVGGRPGEHVRVRIAIYDVRGALVRSLVDADR
                     VSGSYLATWDGLADTGAQAASGVYFCSMRAGDFVASRRMVLLK"
     gene            complement(2155..6213)
                     /locus_tag="VEC56_01030"
     CDS             complement(2155..6213)
                     /locus_tag="VEC56_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXV12764.1"
                     /translation="MTDALLTPDARFARACAAFFAAAVLASALAPAPASAQSFQDPDF
                     YAENAASGATFTAPTTLAFAPDGRVFVGEKSGLVYIIQNDTKLPTPFIDLSWDVLNHH
                     DRGLLGMVLDPDFANTRYVYFLYTFDWNQGGDNQRTDVPGRLVRYQASLADPNVADLN
                     TRTVLIGGTYATGIPACYFSHAPAALRIGSDGTLLIAAGDGASYNQVDPGNLYEDCFG
                     ATKFPEIEDIGAYRSQWIGSMSGKILRVDRATGLGLPSNPYYTGNPADVQSKVWAYGL
                     RNPYRFGVRPNGSTNPADGDPGSLYIGDVGWNLYEEVNVSRAGGGENFGWPCREGPNA
                     QGQYQSGPQPAHHGCSTIGTSANPGPLTNPLTYWHHSNGNNSFPSGITGATAIGGAFY
                     QGTRYPAQYQGVYFYGDYSAQFIKALTVNANDQFVSQMNFGSSMGGVVDIGYNPTDEF
                     LYYINIFTGSVMRIRHVDGDINSPPVASATVEPRFGPLPLNVQFDASASMDPDGNPIT
                     FRWEFGDGQSSTQAVTSHAYTTDGAFVARLTVRDSFGAEATLTFDITAGNTPPNGTIV
                     YPVNGSTAAVGELVVLWGEATDDDTPSEDLTFHWVVSQVHNLHTHPDFFVGDGKLLVF
                     NVTEHGLPHEVNLLRVDMHVSDGSLTDTTTHFVVLARPGETDITGDGTPIALITSPTG
                     SGNPSLSVIHDAVFPIAGSVDPMQQYDTFTGGAPRAVDWIGYSFLETRYFSKLVFQEG
                     IHFADGGWFDDLDVEVLEGSTWLPVANLQVVPPYRANDGINFDMYTLTFTAQAGTAVR
                     VVGAPGGSADYISVGELRVFEIPRADFGADVRIGSPPLTVQFTDLSNVTGATAWLWRF
                     GDGATSAEQNPSHVYSVPGPHTVNLTVSGPSGIYFEEKLDYVFVGTPGITGEYFDGLD
                     FTGPSLTRVDPNVDFDWAFGSPDPSMGTETFSVRWTGWVRPPFTEDYTFRTLTDDGVR
                     LWIDGELIIDQWVLQAATEWTGSVPLVADRMYEVRMEFFDNTAHAVARLSWSSASQPL
                     EVIPQSRLWVRECGQGVGDMDGNGSLTPGDALCVFNTYLNGQVVPGACDAVGYDCEGP
                     AADADCSGLVTPGDALAVYQRYLLGQPVEECLGQAGPALAASFTPARVTTSRRTDAGA
                     VEVAFAVDPTAHFDAFGVRVVVPAGFELAGFERGAGTSDWMLVDARADEDGALWVGGF
                     DTRGLDAASSTELFRLRFRFVGLDADPDLIRFIDFVDDLAGAVEDGGAAPGAPSSFRL
                     YQNHPNPFNPQTSIRFDVPAEAGRVPVTLAIYSVRGERIRTLLHEERAPGIYSVTWDG
                     RDQNGVAVGSGVYFYSLRAGDFTSSRRMVLLK"
     gene            6602..7543
                     /locus_tag="VEC56_01035"
     CDS             6602..7543
                     /locus_tag="VEC56_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12765.1"
                     /translation="MSERSPIRASLMDVRVILEACPPPLADRDKAYAVLRQSFACYNS
                     FHAVSVPDLQDEGEFLRERARVPNEEFARWVRELTEKPLSLYKVVVGCSRAEMEDWLS
                     RAAAIGCRDIFVVGGDSSAKQYRPDALNVGTAAELAAERGFNCGGIIIPTRRAQFVAR
                     PASEDEVDRVLGKIHGHGIRFFSTQILYESEWMCSLLLDLVRRLDKDEMPKIFLTFSP
                     FVVPEDIHFARKTLGVFIPGDVERMLKGARSMREASISALIGVWERLSSFAGQIGFPA
                     DRLGVNVEYLDSRNPRSVDASFELAEEFGRIFRKSTP"
     gene            7562..8662
                     /locus_tag="VEC56_01040"
     CDS             7562..8662
                     /locus_tag="VEC56_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HXV12766.1"
                     /translation="MSARDSIVSVDVWPVDVPLDDPFIISRGRLSAAECAFVRVRLSG
                     GDAGYGEIAPFAALTGETRDGSMADARRLAASLAGRSASQWRELSASLEALAPESPAA
                     RAGLECALVDALARARGAPLHALWSHVEGARPDVRVRETDMTLPMLDDARVAELAQGW
                     YERGFRVLKLKVGDPDVDKDLARVDSLARRYPDVSFVLDANQGYDRARARAFVDALRA
                     WPGRVRLVEQPLSRDDLAGMAELRTLSTVPIVADESVFTLSDVHSVIAARAADVVNLK
                     IMKSGLAQTIEIARAARAAGLGLMIGGMMETRLAMGVSFSLVLGLGGIDHLDLDTPLL
                     MSDDPWVGGYAYDGPRLLPCDEPGLGMQPRES"
     gene            8825..>9227
                     /locus_tag="VEC56_01045"
     CDS             8825..>9227
                     /locus_tag="VEC56_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV12767.1"
                     /translation="MAEVTRRIMIEAKGLSKYYGPFVAIKDVSFSIPEGQIVSFLGPN
                     GAGKSTTMKILAGYLAASEGTARIAGFDMTVERLSGARQLGYLPENGPLYNDMTPFDL
                     LTFFGQARQLDPARLKNRIGVVVEQCALQAVI"
BASE COUNT         1492 a         2981 c         3241 g         1513 t
ORIGIN      
        1 tggcggcttt catccgcgtc tcgcgggatt gcgccagggt gaggaatggg gccgcccccg
       61 ttgcaatgac cacgccgaga aagcggcggc ggggaagcga gcgcgcggaa gtttcgatac
      121 gcgattgcat gtttgctggc gtagcgcgtc tgagggacgg tggcaagacg gatttttcga
      181 cagatcaccc ttgcttaacg gagcgagtgc cttttcaata tgtggccctc ggtggctggg
      241 tagctcagtc ggtagagcag gggactgaaa atccctgtgt cggcggttcg attccgtccc
      301 ttcgcaccat tctcttttct tcccgtaatt acttgagcag gaccatgcgg cgcgaggcga
      361 cgaagtcgcc cgcacgcatc gagcagaagt agaccccgga ggcggcctgc gcgccggtat
      421 cggcgaggcc gtcccaggtg gcgaggtagg acccggagac gcgatccgcg tcgaccagcg
      481 agcgtaccaa ggcaccgcgc acgtcgtaga tcgcgatgcg gacgcgcacg tgctcgcccg
      541 gtcggccgcc cacgtcgtag cgcacgtggg tgctgggatt gaacgggttg gggtagttgg
      601 ggtgcaggct gtggatgccc acctgcggct cgacgtcggt gaccgcgccc atggtggcac
      661 cggtgaagtc gtccacgaag ttggagagta cgagccctgt gaccgtcgcc ggagagccca
      721 cgaaatcgaa gaagatgcgg cacacgtcgg cggttcccgg cggcgcggtc atggtgggat
      781 cgaagccgcc cagcacgatt tgtcccacgg ccacggaccg cccgtcgatg ctgtgccagc
      841 cggctgtcag atatccaggc tccacgcggt tgaacaccag ctcggaagcg ggaaacgaga
      901 gtcgcgctcc gaaggcgtcc aagtccgagg cgtcctcgac gaccagaagc acctggaggc
      961 gcggggtccc gccgtcgtcg acgatgtact gcaccagccc cagctcgtgg ggcggcgacg
     1021 gcaccgggtc cgggggcgcg aagcacggcg cgggggccag gctcgcggcc gcgcgaatct
     1081 cgatcgcgcg cgcatccgcg ggcgtcaccg cgccgctgca attgacgtcg gcggccgaaa
     1141 gctcgcaggt cgtgctcggc gagtcgcacc ccggcagcac ggcttggccg gagaggaaca
     1201 tgtcgaacgc gcacgcaaca tcggaggcgt ccagcagccc ggtcgcatcg acgtcgccga
     1261 tcccctgcgc gcacagcgac gcgatcaggc ggttggacgg aatgatctgc ttgccctggc
     1321 tgggcgacga ccacgacagt tgcgccaccg cctcgcccgc ttcttcgaac atctccatgc
     1381 gaatcgacgc cgggacgccg gccgtgagcg cgatgctgcc gctgcgctcg atggcggcct
     1441 ggggaatcca atagtcgatg atcaactggt cgttgaccca caagcgcacg ccgtcgtcgg
     1501 tgatcgtgta gaacgtgtag gtctgcgtga acgccgggtc gacgtagccg gtccagcgaa
     1561 tcgaaaagtc gttggtgccc atcgacgggt cgggcgatcc cagggcccaa tcgaaattga
     1621 cggtcgcgtc gacgcggcgc aggagcgtgc cggtgaagtc gatgttgttg tagtactcgc
     1681 cgagcagccc ggccccgccc ggggcgacca ccacctggaa ggtggccggg gtcgtggtcg
     1741 ccgccgtgat gtcgtcgacg aagttcgaga gcgcgatggc tccgctggtg agcaggttgg
     1801 tctcgaccag gaaggcgacg cgcaccagga cgccgccggc cgtggtggga gtggtgtgaa
     1861 acccgccgac ttgaaccacg ccgggagtcg tctcggcgcc cgagacggcg atccagccgt
     1921 cggtgagcgt tcccgcgacc gataccgact ggtaggcgag cagcgcctcc ggatacgaaa
     1981 gacggaagcc gaacgcgtcg acgggagcgg ctcccgggtt ggtgagcacg acgtcgacga
     2041 gcacggtctg attgacggaa ccgctggtcg gctgggggct gaccgacacc tgcgcgtgcg
     2101 caaccggcgc ggcgcacacc agtgcgacca tggacacgat gcggagcgcg cgtgtcattt
     2161 cagcagcacc atccgccgcg agctcgtgaa atcaccggcg cgcaggctgt agaaatacac
     2221 gcccgatccg accgcgacgc cgttctgatc gcgaccgtcc cacgtgacgc tatagatgcc
     2281 gggcgcgcgc tcttcgtgca agagggtgcg aatgcgctcg ccgcgcaccg agtagatcgc
     2341 cagcgtcacc ggcacgcgac ccgcctcggc gggcacgtcg aagcgaatcg acgtctgcgg
     2401 attgaacgga ttgggatgat tctggtagag gcggaacgac gacggcgcac ccggcgcggc
     2461 gccgccatct tccaccgcgc ccgcgaggtc gtcgacgaaa tcgatgaagc gaatgagatc
     2521 cgggtcggca tcgaggccga caaagcggaa gcgcaagcgg aacagctcgg tcgagctcgc
     2581 cgcgtccagg ccgcgcgtgt cgaagccgcc cacccacagc gcgccgtcct cgtcggcgcg
     2641 cgcgtcgacg agcatccaat cgctggttcc cgcgccgcgc tcgaagcccg cgagctcgaa
     2701 tcccgccgga accaccacgc gcacgccgaa ggcgtcgaag tgagcggtcg ggtcgacagc
     2761 gaacgcgacc tccaccgcgc ccgcgtcggt gcgtcgcgac gtcgtgacgc gcgccggagt
     2821 gaacgacgca gccagcgcgg ggccggcttg ccccaggcac tcttcgaccg gctgccccag
     2881 caggtagcgc tgatagaccg cgagggcgtc gcccggtgtc accagcccgc tgcagtcggc
     2941 gtcggccgcg gggccttcgc aatcgtagcc gaccgcgtcg cacgcgccgg ggaccacttg
     3001 gccgttcaaa taggtattga agacgcacaa cgcgtcaccc ggtgtgagcg agccgttgcc
     3061 gtccatgtcg cccacaccct ggccgcactc gcgcacccac aggcgcgatt gggggatgac
     3121 ctcgagcggt tggctcgcgg acgaccagga gagccgcgcc accgcgtgcg cggtgttgtc
     3181 gaaaaactcc atgcgcactt cgtacatgcg atcggcgacg agcggaaccg aacccgtcca
     3241 ctcggtcgcg gcctgcagga cccattggtc gatgatcaac tcgccgtcga tccacaggcg
     3301 cacaccgtcg tcggtgaggg tgcggaacgt gtagtcttcc gtgaacggcg gccgcaccca
     3361 gccggtccag cggaccgaga aggtttcggt ccccatcgag gggtcgggcg aaccgaacgc
     3421 ccagtcgaag tcgacgttgg gatcgacgcg cgtcaggctc gggcccgtga aatcgagtcc
     3481 gtcgaaatac tcgccggtga tccccggtgt acccacgaac acgtagtcca gcttctcttc
     3541 gaagtagatg ccggatggcc ccgacacggt gaggttcacg gtgtgcggac cggggacgct
     3601 gtacacgtgg gacgggttct gctccgcgct ggtggccccg tcgccgaagc gccacagcca
     3661 cgcggtggcg ccggtgacgt tggacaggtc ggtgaactgg acggttaggg gcggcgagcc
     3721 gatcctcacg tcggcaccga agtccgcgcg gggaatctcg aacacgcgca gctcgccgac
     3781 cgagatgtag tccgcgcttc cgcccggtgc tcccacgacg cgcaccgccg tcccggcttg
     3841 ggccgtgaac gtcagcgtat acatgtcgaa gttgatgccg tcgttggccc ggtagggggg
     3901 aaccacctgc agattcgcga ccggcagcca cgtgctgccc tccaagacct cgacgtcgag
     3961 gtcgtcgaac cacccgccgt cggcgaagtg gattccttcc tggaacacga gcttcgagaa
     4021 gtagcgcgtc tcgagaaacg agtagccgat ccaatccacg gcgcgcgggg cgccgccggt
     4081 gaaggtgtcg tactgctgca tgggatcgac actgcccgcg atcgggaata ccgcgtcgtg
     4141 gatcaccgag aggctggggt tgccgctccc ggtcggagac gtgatcagcg cgatgggcgt
     4201 gccgtcgccc gtgatgtcgg tttcgcccgg gcgcgccagc accacgaagt gcgtggtcgt
     4261 atcggtgaga ctcccgtcgc tcacgtgcat gtcgacgcgc agcaggttca cctcgtgcgg
     4321 aagcccgtgc tcggtgacgt tgaagaccag gagttttccg tcgcccacga agaagtccgg
     4381 gtgggtatgg agattgtgga cctggctcac cacccaatgg aacgtgaggt cctcggaggg
     4441 cgtgtcgtcg tcggttgcct cgccccacag caccacgagc tcgcccactg cggcggtcga
     4501 tccgttgacc gggtacacga tggtgccgtt gggcggcgtg ttgccggccg taatgtcgaa
     4561 ggtgagggtc gcttccgctc cgaacgaatc gcggacggtc aggcgcgcga cgaaggcgcc
     4621 gtcggtggtg taggcgtgcg acgtcacggc ctgagtcgac gactgtccgt cgccgaactc
     4681 ccaccggaac gtgatcggat tgccgtcggg gtccatcgac gcgctggcgt cgaactgcac
     4741 gttcagcggc agggggccaa agcgcggctc gaccgtggcg ctcgcgacgg gcgggctgtt
     4801 gatgtcgccg tcgacgtggc gaattcgcat caccgagccg gtgaagatat tgatgtaata
     4861 gagaaactcg tccgtggggt tgtaaccgat gtcgaccacg ccacccatcg acgacccgaa
     4921 gttcatctgc gagacgaact gatcattggc gttgacggtg agcgccttga tgaactgcgc
     4981 ggagtagtcg ccgtagaagt acacgccttg gtactgcgcc gggtacctcg tgccctggta
     5041 gaacgcgccg ccgatcgcgg tggcacccgt gatgccggac gggaacgagt tgttgccgtt
     5101 ggagtggtgc caataggtga gcggattcgt caggggcccc gggttggcag atgtcccgat
     5161 ggtcgagcac ccgtggtggg ccggctgcgg tccgctctgg tactgaccct gcgcgttggg
     5221 tccttcgcgg cacggccacc cgaagttttc gccgccgccc gcgcgcgaaa cgttcacttc
     5281 ttcgtacagg ttccacccga cgtcgccgat atagagcgag ccgggatctc cgtcggcggg
     5341 gttggtactc ccattggggc gcacaccgaa gcggtacggg ttgcgcaggc cgtacgccca
     5401 caccttggat tggacgtcgg ccgggttgcc ggtgtagtac gggttcgaag gcagtcccaa
     5461 gccggtggcg cgatcgacgc gcaggatctt gcccgacatg ctcccgatcc actgcgagcg
     5521 atacgctccg atgtcttcga tctcggggaa cttggtggca ccgaagcagt cctcgtatag
     5581 gttgcccggg tcgacttggt tgtagctggc gccgtcgccg gccgcgatca agagcgtgcc
     5641 gtcggagccg atgcgcagcg ccgctggcgc gtgcgagaaa tagcacgccg gaatgcccgt
     5701 cgcgtaggtg ccgccgatga gcaccgtgcg cgtattcagg tcggcgacgt tggggtcggc
     5761 gaggctggcc tggtagcgca ccaggcgccc gggaacgtcg gtacgctggt tgtcgccgcc
     5821 ctggttccag tcgaacgtgt agagaaagta gacgtagcgc gtattcgcga agtcggggtc
     5881 gagcaccatc cccagcaacc cgcgatcgtg gtggttgagc acgtcccacg agagatcgat
     5941 gaacggcgtc gggagcttgg tgtcgttctg aatgatgtac accaggccgc tcttctcgcc
     6001 cacgaacacg cggccgtcgg gcgcgaaggc gagggtggtg ggggcggtga aggtggcgcc
     6061 ggaggccgca ttctcggcgt agaagtcggg gtcctggaag ctctgcgccg acgccggggc
     6121 gggcgcgagc gcggaggcca gtaccgcagc cgcgaagaac gcggcgcaag cgcgcgcgaa
     6181 gcgggcgtcc ggggtgagaa gagcgtcggt catcgtgttg gtttgaggtc gacctcggta
     6241 gtaccgaggg tctccaccgg gtcgagggca acgcctgtgc cacgcaccag catgctgtaa
     6301 acactcgact tgcgcgatgg cctggcggct tcactgggga gtctgccccg gcacagacgc
     6361 ggtttttccc aggtgcgtat ccgggggttg acggggcttc cccggtgccg cgtgtttcgg
     6421 ggtggtgcag ccgggctgga cggacgccgc gcgccgcgcc gcctcgcgcg tttccccgtt
     6481 gcgaccggca gggtcgtgac gtaagattcg gtccgtcgtc acgccgcggt cgcgccgccc
     6541 gttatttcgc gcgggtcggg agcaccgcgg cgtcgtcgca gaaggggatt ccgtggacac
     6601 gatgagcgaa cgatcgccga tcagggccag cctcatggac gtgcgggtga tactcgaagc
     6661 gtgcccgccg ccgttggccg atcgggacaa ggcgtacgcg gtgctgcgcc agagtttcgc
     6721 gtgctacaac tcgttccacg cggtcagcgt ccctgacctc caggacgagg gcgagttcct
     6781 gcgcgagcgc gcgcgggttc ccaacgagga gttcgcccgc tgggtgcgcg agctgaccga
     6841 gaagccgctg agcttgtaca aggtggtggt ggggtgttcg cgcgccgaga tggaggactg
     6901 gctctcgcgt gcggccgcca tcgggtgccg agacatcttc gtggtgggag gagactccag
     6961 cgccaagcag taccgccccg acgcgctcaa tgtgggcacg gcggccgagc tcgccgccga
     7021 gcgcgggttc aactgcggcg gtatcatcat cccgacgcga cgcgcgcaat tcgtggcgcg
     7081 accggcctcc gaggacgaag tcgaccgcgt gctgggcaag atccacggcc acggcatccg
     7141 cttcttctcg actcagatac tgtacgagag cgagtggatg tgtagcttgc tgctcgatct
     7201 ggtgcggcgg ctcgacaagg acgagatgcc gaagatcttc cttaccttct cgcccttcgt
     7261 cgtcccggag gacatccatt tcgcgaggaa gacgctcggc gtgttcatcc ccggcgacgt
     7321 cgagcgcatg ctcaagggcg cgcgcagcat gcgcgaagcc tcgatctccg cgctgatcgg
     7381 cgtgtgggag cggctgtcgt cgttcgcggg ccagatcgga tttcccgccg accgcctcgg
     7441 tgtcaacgtc gagtacttgg actcgcgcaa cccgcgctcc gtcgacgcgt ccttcgaact
     7501 ggccgaggaa ttcggtcgca tctttcgcaa gtcgaccccg taggcccgtg cccgcctcac
     7561 cgtgagcgcg cgcgactcga tcgtctccgt cgacgtatgg ccggtcgacg ttcccctcga
     7621 tgaccccttc ataatctcgc gcggccgttt gtcggcggcc gagtgcgcct tcgtgcgggt
     7681 tcgcttgtcc gggggcgatg ccggctacgg agagatcgcg ccgtttgcag ccctcacggg
     7741 cgagacgcgc gacgggtcga tggcggacgc gcgccggctg gccgcttcac tcgcggggcg
     7801 cagcgcctcc caatggcgcg agctgtccgc gagcctcgag gcgctcgcgc ccgagtcgcc
     7861 ggccgcgcgg gccgggctgg agtgcgcgct ggtcgacgcg ctggcgcgcg cgcgcggggc
     7921 gccgctgcac gcgctgtggt cgcacgtcga gggggcgcgg cccgacgtgc gcgtgcgcga
     7981 gaccgacatg acgctcccca tgctcgacga cgcgcgcgtc gcggagctcg cccagggctg
     8041 gtacgaacgc ggctttcgcg tcctcaagct caaggtcggt gaccccgacg tcgacaagga
     8101 cctcgcgcgc gtggattcgc tggcgcgccg ctaccccgac gtgtcgttcg tcctggatgc
     8161 aaaccagggc tacgaccgcg cgcgggcgcg cgccttcgtc gacgcgctgc gcgcgtggcc
     8221 cgggcgggtg cgcctggtcg agcagccgct ctcgcgcgac gacctggccg gaatggcgga
     8281 actgcgcact ctctcgaccg ttcctatcgt cgccgacgag tcggtgttca cgctctccga
     8341 tgtgcacagc gtgatcgcgg cgcgagccgc cgacgtggtg aatctcaaga tcatgaagtc
     8401 gggactcgcg cagacgatcg aaatcgcgcg cgcggcgcgg gccgcgggcc tcggtctcat
     8461 gatcggcggc atgatggaga cgcggctggc gatgggggtg tcgttttcgt tggtgctggg
     8521 gttggggggc atcgaccacc tcgacctcga cacgccgctc ctcatgtccg acgacccctg
     8581 ggtcggcgga tacgcctacg acggcccgcg cctgctcccg tgcgacgaac ccggcctcgg
     8641 catgcagccg agggagagtt aggctccttt ttttgggggt tgcgtggcgc ccgccggccg
     8701 cggtagcatt cgcggtcgtt tctcgaccgc accgtccccg agcgaaactc tccggggcag
     8761 cgcttgtata accgtccaaa ttttcgcacc ccgacgtcga accgtcgccc aaggagtctc
     8821 tgatatggcg gaagtgacgc gtcgcatcat gatcgaagcg aagggcctct cgaagtacta
     8881 cgggccgttc gtcgcgatca aagacgtttc gttctccatt cccgaaggcc agatcgtgtc
     8941 gttcctcgga cccaacgggg ccgggaagtc gaccaccatg aagatcctag ccggctacct
     9001 cgccgccagc gaaggcaccg cgcgcatcgc cgggttcgac atgaccgttg agcgcctctc
     9061 gggcgcccgt cagctcgggt acctgcccga gaacggcccg ctctacaacg acatgacgcc
     9121 gttcgatcta ttgacgttct tcggccaggc gcgccagctc gacccggccc gcctgaagaa
     9181 ccggatcggc gtggtcgtgg agcagtgcgc gctccaggcc gtgatcg
//
LOCUS       DASPGK010000041         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1064091, whole genome shotgun sequence.
ACCESSION   DASPGK010000041 DASPGK010000000
VERSION     DASPGK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064091"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VEC56_01050"
     CDS             <1..607
                     /locus_tag="VEC56_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV12768.1"
                     /translation="PAGTTLMTTQDDETLVRRFRRGEREVFGEILDRYEKRVFNVAWR
                     IVHDVEDARDVTQTVLTKVFTNIARYDARFCFSTWIYRIAVNESIRLLKKQRTQEEVD
                     DTIPSRFRDAHELLCGSELSAAIQRCLNRLRPEYRVVVVLRHFADCSYREMSRILDIP
                     EKTVKSRLFDARRMLRNELVDCGALAGPSAHEARRRRSDSA"
     gene            573..1334
                     /locus_tag="VEC56_01055"
     CDS             573..1334
                     /locus_tag="VEC56_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12769.1"
                     /translation="MKHAADDLIQRSLDGGLSEREQRRLRRILEADPSARAVRDELEA
                     VVDLLGQVEEVAPPPDLRSGILAALSDPPVRERGWLSMLERGGGSWLPRAAALTAAAA
                     IAAVLVVVLVPTYRIPREGEGLAGTLAPAHDAEPSIVRLDLRQEWISGTLRAEATPSG
                     VALELAATTAGSVSANVLYEPTELRFIASSAGEATVQGSGRLEIDPLQSGELTLFFER
                     IGTGESRLVVEFADSGGRRHQRVVLLPGARAEHGE"
     gene            1309..>2057
                     /locus_tag="VEC56_01060"
     CDS             1309..>2057
                     /locus_tag="VEC56_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12770.1"
                     /translation="MLGRSTGSEPDQLLTSYEVIRGSTAVRRTNRPEGGKPMAEQGNR
                     KFVFIGIGGLVVAAALVGYFGFIYPPNGQDLTGTIAPANRHQTTQITDADVQLTDEEI
                     AAVMQTDEFDRIIKDADLRKALASPDVQAALANPDFARALANADFARALGSADFARAL
                     GNADFARAMGHGDFSRAMASGDFARAMGNADFAKALGNADFARAMGNADFAKALGNAD
                     FARALGNADFARAMGGADFARAMGNADFAKAL"
BASE COUNT          361 a          665 c          690 g          341 t
ORIGIN      
        1 accggcaggt accaccttga tgacgacgca ggacgacgag actctggtcc gacgtttccg
       61 aagaggggaa cgcgaggtct tcggagagat cctcgatcgc tatgaaaaga gagtcttcaa
      121 cgtggcctgg cggatcgtgc acgacgtgga ggacgcgcgc gacgtcaccc agacggtcct
      181 caccaaggtt ttcaccaaca tcgcgcgtta cgatgcgcgt ttttgcttct cgacctggat
      241 ctaccgcatc gccgtcaacg aatcgatccg gttgctcaag aagcagcgca cgcaagaaga
      301 agtcgacgac acgatcccct cgcggttccg cgatgcccac gagctgctgt gcggatccga
      361 gctttctgcg gcgatccaac gatgtctgaa tcggctgcga ccggagtacc gcgtggtcgt
      421 cgtgctgcga cacttcgcag actgcagcta tcgcgaaatg agccgcatcc tcgacatacc
      481 cgagaaaacg gtgaagtcgc gtttgttcga cgcccgaagg atgctgcgca acgagctcgt
      541 cgactgcggc gcgctcgcgg ggccctccgc tcatgaagca cgccgcagac gatctgattc
      601 agcgtagcct cgacggcggt ctttccgagc gcgagcagcg ccggctgcgg aggattctgg
      661 aggccgaccc gtcggcccgg gcggtgcgag acgagctcga ggccgtcgtc gacctgctcg
      721 ggcaggtgga agaagtggcg cctccgcccg atctccgaag cggcatcctc gcggccctgt
      781 cggatccgcc cgtgagagag cggggttggc tgtcgatgct cgagcggggc ggtggcagtt
      841 ggttgccgcg cgccgcggcg ctgaccgccg cggcagcgat agccgccgtg ctggtggtcg
      901 ttcttgtgcc gacctatcgg atcccgcggg aaggagaggg gctggcggga acgctcgcac
      961 ccgcgcacga cgcggagccg tcgatcgtcc ggctcgacct ccgacaggag tggatctccg
     1021 gaaccctgcg ggccgaggcc acaccgagcg gcgtcgccct cgagctggcc gcgaccacag
     1081 ccgggtccgt cagcgcaaac gtgctctacg agccgaccga gcttcgcttc attgcctcct
     1141 cggccggcga ggcgacggta caaggctccg ggcggctcga gatcgacccg ctgcagtcgg
     1201 gcgagctgac gctcttcttc gagcgaatcg ggaccggaga gtcgcgtttg gtggtggagt
     1261 tcgccgattc cggcgggcga cgtcatcagc gggtcgtctt gcttcccggt gctcgggcgg
     1321 agcacgggga gtgagccgga ccaacttttg acgtcttatg aggtaattag gggtagcacg
     1381 gcggttcgcc gtaccaaccg gcccgaagga gggaaaccga tggcagagca agggaatcgc
     1441 aaattcgtct ttatcggaat cggagggctc gtcgtcgcgg cagcgctcgt cgggtacttc
     1501 gggttcatct acccgccgaa cgggcaggac ttgaccggga ctatcgctcc cgcaaatcgc
     1561 caccagacga cgcagatcac cgacgcggac gttcagctga cggacgaaga gatcgccgcc
     1621 gtcatgcaga ccgacgagtt cgaccgcatc atcaaggacg ccgacctgcg aaaggcgctg
     1681 gcaagtccag acgttcaggc cgcgctcgcc aatccggact tcgcacgcgc cctggccaac
     1741 gcggactttg cgcgtgcact cggtagcgcc gatttcgcac gtgcgctggg caacgcagac
     1801 ttcgcccgtg ccatgggcca cggcgacttc tctcgcgcga tggccagcgg cgacttcgct
     1861 cgcgccatgg gcaacgcaga cttcgccaag gcgctcggca acgccgactt cgctcgcgcg
     1921 atgggcaatg ccgacttcgc caaggcgctc ggcaatgcgg acttcgcccg cgcactgggg
     1981 aacgccgact tcgctcgcgc gatgggtggt gccgacttcg cccgtgcgat gggtaacgcc
     2041 gacttcgcca aggcgct
//
LOCUS       DASPGK010000042         5989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_361656, whole genome shotgun sequence.
ACCESSION   DASPGK010000042 DASPGK010000000
VERSION     DASPGK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361656"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2215)
                     /locus_tag="VEC56_01065"
     CDS             complement(<1..2215)
                     /locus_tag="VEC56_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXV12771.1"
                     /translation="MNPIRRYLHWLHGQWPAGAVERLPECNQDGSTRIPGLYIVGDLR
                     GVPLLKFAADSGARAIHAIRAMRGFVAANGEAGADVVDVAILGGGVSGAAAAMEATKH
                     GLTFRWFEASEPFSTIVNFPRRKPIFTYPKSMTPAGELRVSADVKEALVEELVAQARR
                     AGVTPETAHVARVERRGDGFDVALSDARTIRARHVVCALGKSGDFRRLNVPGERLDKV
                     TNRLHDPADFAGRRVLVVGGGDSALETAVALTDAGARVTLSYRGDTFARPKHENVEAL
                     ARAQNAGRLRVLMSSTVKEIRPDAVDIATGDGRVEPLPNDDVFVMIGREAPLGFFRRS
                     GIHLHGERSRRTWVAFASFVAFCVALYNWKSGGALGNLFYQRHWWPVSIADAARALAP
                     GSLGSVLLTSASSPSFWYTLAYSALVTVFGVRRIRRRRTPYITLQTSVLMAIQVLPLF
                     ILPEILLPYLGANGLLPAGVLDALFPVVDYGHGREYWRAYGFVLAWPLNVYNVFTNQP
                     LGWWLAIAFVQTCVLIPVLIYFWGKGAYCGWICSCGALAETLGDRHRHKMPHGPFWNR
                     FNMVGQVFLAFAFALLIFRAIGWIWPGSFAGRWYDYLLTKAPVINYVWFVDLLWAGIL
                     GVGLYFWFSGHVWCRFACPLAALMHIYTRFSRFRILADKKKCISCNVCTSVCHQGIDV
                     MQFANRGAPMEDPECVRCSACVQSCPTGVLSFGQVDRSGRVVSRDTLPASPVRMRE"
     gene            2303..3031
                     /locus_tag="VEC56_01070"
     CDS             2303..3031
                     /locus_tag="VEC56_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HXV12772.1"
                     /translation="MKRLLCVFAHPDDESYGPGGTIARCALAGADVFITMFTAGEAGT
                     IGVSKALARDELARLRRAELAAACEALGVREHRVLGAPDGGVASLDPAWAIAEILADI
                     RRFRPQVAITFHRGGVSGHRDHIAVAGFLDRAIAQAGADGPQASYEWGIPHRRSQLYQ
                     RPSLVPLEDHEIAAVVDIDDEAMERKLDAIRRHQTQIEFFVSLEQKFDYRAVSRPEHF
                     ARGRSRIAPAPSPVGDLFDGIEVD"
     gene            3033..3467
                     /locus_tag="VEC56_01075"
     CDS             3033..3467
                     /locus_tag="VEC56_01075"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HXV12773.1"
                     /translation="MWDALGRMHPFLVHFPVALILTAFVAESVAAARRDERWGDAARF
                     MITAAAWLAIPAAVAGFARADAIVIKPELQPTFAVHRIAGIATPVLAFLAAGLVAGVR
                     RSGQIWELFLYRAVLALAAVAAAIAGHHGGEMVYGTDFFPLW"
     gene            3600..4865
                     /locus_tag="VEC56_01080"
     CDS             3600..4865
                     /locus_tag="VEC56_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HXV12774.1"
                     /translation="MDKNALAAVAARIDGYRDQVIDLQRELCKRPALDPTSGGIGEEE
                     KAAWLESYLRGLGLTVARYDAPDARVPSGRRPNLVTYYPNNPSSGPRLWIIAHTDVVP
                     PGDQALWTGDPWTLRIDGDRLIGRGTEDNQGGLSAAVMAVRAFIETRTQPAIPFALAF
                     VADEETGSVYGLRYLLEKHRSLFRKDDLIIIPDSGNPDGTELEIAEKGILWVRFRTLG
                     KQTHGSTPATGINAHKAAAWMIVRLESLYTKFKKRNPLFHPSNSTFEPTKKESNVPNI
                     NTIPGEDVFYFDCRVLPAYKLDEVIRAIKAVVKETERKFKVKVEVTFPQKESPAPPTP
                     ADSPVAVAITRAVKELRKKRPKAIGIGGGTVAKYFREAGFPCAVWSTMDERAHRPDEH
                     VRVSHILADAKAFAHIALAGGAAPGAKKK"
     gene            4948..5817
                     /locus_tag="VEC56_01085"
     CDS             4948..5817
                     /locus_tag="VEC56_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXV12775.1"
                     /translation="MQGIAFGPTIRLLRQAKGISLREMARQLGVSPAFLSQIEAGRQH
                     KIPRARIVQVAEMLGVSEGYLLGTAKQVHPDLTKFLSETPEAAEFMVTAMRSGMIAQD
                     FTNLREIVSDKKLRASVLKPVAAPKKKSGPSHTLDGYLDASLCLAGVAVQNKERLFQR
                     FVKAVSKKSKGISGEVLLDRLMARERQAPTSIGGGVAIPHAFLQGIEKPLVGAFLLQR
                     AIPYGPTESDRVKTVFFLVGRDGNTEHHLPILARIARLCSTPEFLQVLAKAKNGRDIE
                     RTILAWDARIGSA"
BASE COUNT         1056 a         1983 c         2024 g          926 t
ORIGIN      
        1 gttcgcgcat gcggacgggc gaggccggca gcgtgtcgcg ggagacgacg cggcccgatc
       61 gatccacctg cccgaacgag agcactcccg tgggacagct ctgcacgcag gccgagcagc
      121 ggacgcactc cggatcctcc atgggagccc cgcggttcgc gaactgcatg acgtcgatcc
      181 cctggtggca gaccgaggtg cagacgttgc aagagatgca cttcttcttg tcggccagga
      241 tgcggaagcg gctgaagcgc gtgtagatgt gcatcaatgc ggccagtggg caggcgaagc
      301 ggcaccacac gtgaccgctg aaccagaagt agagcccgac cccgagaatg ccggcccaga
      361 gcagatcgac gaaccagacg tagttgatga cgggggcttt ggtgagcagg taatcgtacc
      421 atcgtcccgc gaaggagccg ggccagatcc agccgattgc gcggaagatc aacagcgcga
      481 acgcgaacgc gagaaacacc tgccccacca tgttgaaccg attccagaac ggcccgtgcg
      541 gcatcttgtg gcggtgacgg tcgccgagcg tctccgcgag tgcgccgcac gagcagatcc
      601 agccgcagta cgcgcccttg ccccagaagt agatgagcac gggaatcagc acgcacgtct
      661 gcacgaaggc gatcgcgagc caccacccga gcggctggtt ggtgaacacg ttgtagacgt
      721 tgagcggcca cgcgagcacg aacccgtacg cgcgccagta ctcgcggccg tggccgtagt
      781 cgaccacggg gaagagcgcg tcgagaacgc ccgcgggcaa gagcccgttc gcgccgaggt
      841 aggggagcag tatttcgggc aggatgaaga gcggcagcac ctggatggcc atcagcaccg
      901 aggtttgcag cgtgatgtag ggggtgcgcc gccgtcgaat ccgccgcacg ccgaacacgg
      961 tgacgagcgc ggagtacgcg agtgtgtacc agaacgacgg actggaggcg gacgtgagca
     1021 gcacggagcc cagcgaaccg ggcgccagcg cccgcgcggc atccgctatc gagaccggcc
     1081 accagtgcct ttggtaaaag agattcccga gcgccccgcc cgacttccag ttgtagaggg
     1141 cgacgcagaa ggcgacgaag gacgcgaacg cgacccacgt ccgccgcgaa cgctcgccgt
     1201 gcaggtggat ccccgagcgc cggaagaacc ccagcggcgc ctcgcggccg atcatgacga
     1261 acacgtcgtc gttggggagt ggctcgacgc gcccgtcgcc cgtcgcgata tcgacggcgt
     1321 ccgggcgaat ctccttgacg gtcgacgaca tcagcacgcg cagcctgccg gcgttctgcg
     1381 cgcgcgcgag tgcctccacg ttttcgtgtt tgggacgcgc gaaggtgtcg ccgcggtagg
     1441 agagcgtgac gcgggccccc gcgtcggtca gtgccaccgc ggtctcgagc gcgctgtcgc
     1501 cgcccccgac caccagcacg cgccggcccg cgaagtccgc ggggtcgtgc aggcggttgg
     1561 tcaccttgtc gagcctttct ccgggaacat tcaggcggcg gaaatcgcca ctctttccca
     1621 gcgcgcacac tacgtggcgc gctcgaatcg tgcgcgcgtc cgagagcgcc acgtcgaagc
     1681 cgtcgccgcg tcgttccacg cgcgcaacgt gggccgtctc gggcgtgact cccgcgcggc
     1741 gcgcttgcgc gacgagctct tcgaccagcg cttctttgac gtcggccgag acccgcagct
     1801 cgcccgcggg cgtcatcgac ttgggatagg taaagatcgg cttgcgccgc gggaaattga
     1861 cgatggtgga gaagggctcg ctggcttcga accagcgaaa cgtgagtcca tgcttggtcg
     1921 cttccatggc cgccgccgcg ccggagacgc cccccccgag gatggcgacg tcgaccacgt
     1981 ccgcgcccgc ttccccgttt gcggcgacga acccgcgcat ggcgcgaatg gcgtggatcg
     2041 cgcgcgcgcc gctatcggcg gcgaacttga gcagcggcac gccgcgcaag tcgccgacga
     2101 tgtacagccc cggaatgcgg gtggagccgt cctggttgca ctcgggcaac ctttcgacgg
     2161 cgccggcggg ccattgcccg tgcagccagt ggaggtaacg acgaatcggg ttcatgcggc
     2221 gagccgatta tgggtcgaag cagcccgacc acgcaaccgt tgactccgca ggggcgcgac
     2281 ggtacgttcc ggcggacgat tcatgaaacg attgctgtgc gtattcgccc accccgacga
     2341 cgagtcctac gggcccggtg ggaccatcgc gcgctgcgcg ctggccggcg ctgacgtgtt
     2401 catcacgatg ttcacggcgg gcgaagcggg caccatcggc gtgtccaagg cgctcgcgcg
     2461 cgacgagctg gcacgcttgc ggcgcgcgga gctcgcggct gcgtgcgagg ccctgggcgt
     2521 gcgcgagcac cgcgtgctgg gcgcgcccga cggtggcgtc gcttccctcg acccagcctg
     2581 ggcgatcgcg gagattctgg cggacatccg gcgcttcagg ccgcaggtcg cgatcacctt
     2641 ccaccgcggc ggcgtctctg ggcatcgcga ccacatcgcg gtcgcaggct ttctcgaccg
     2701 cgcgattgcg caggcgggcg ccgacggccc gcaggcgtcg tacgaatggg gtattcccca
     2761 ccgcaggtcg cagctctacc agcggccaag tttggtcccg ctcgaggatc acgagatcgc
     2821 cgcggtggtg gacatcgacg acgaggcgat ggagcgcaag ctcgacgcca ttcgtcgcca
     2881 ccagacccag atcgaattct tcgtgagtct cgagcagaag ttcgactatc gtgcggtttc
     2941 gaggccggag cacttcgcgc gcggccgctc ccggatcgcg cccgctccct cgccggtggg
     3001 cgatttgttc gacggaatcg aggtcgattg agatgtggga cgcgctgggt cggatgcacc
     3061 ccttcctggt ccatttcccg gtggcgctga tcctgaccgc cttcgtcgcg gagagcgtgg
     3121 cggccgcgcg gcgcgacgag cgctggggcg acgcggcccg cttcatgatc acggccgcgg
     3181 cttggctcgc gattccggcc gccgtggcgg ggttcgcccg cgccgacgcg atcgtgatca
     3241 agcccgagct gcagccgacc ttcgccgtcc accgcatcgc ggggatcgcc acgccggtgc
     3301 ttgcattttt ggccgcgggg ctggtggcgg gcgtccgccg cagcggccaa atctgggagc
     3361 tgttcctcta tcgcgcggta ctggcgctgg ccgcggtcgc ggccgcgatc gccggacacc
     3421 acggcggcga gatggtgtac ggcacggact tctttcctct gtggtaaacg gttgactcga
     3481 ggcgtggagc cgcggtaagc tgccactctc gaccgcacct catacgggcc ccgaaccacc
     3541 caaagaagtg tgacgaacca aacgacgcgc gtcagcgcgt ctatccttct cggaggacca
     3601 tggacaagaa cgccttagcg gccgtcgcgg cgcgtatcga cggctatcga gaccaggtta
     3661 tcgatctgca gcgagagctg tgcaagcgtc ccgcactcga ccccaccagt ggtggcatcg
     3721 gcgaggaaga aaaagcggcc tggctggagt cgtacctgcg cggcttggga ttgacggtcg
     3781 cgcgatacga cgcgcccgac gcgcgcgtcc ccagcggtcg gcgcccgaat ctcgtcacgt
     3841 actatccgaa caaccccagc tcgggcccgc gcctatggat catcgcgcac accgacgtgg
     3901 tcccgcccgg cgatcaggcc ctgtggaccg gtgacccgtg gacgctgcgt atcgacggcg
     3961 accggctgat cggacgcggc accgaagaca accagggcgg gctatcggcc gcggtgatgg
     4021 cggtgcgcgc gttcatcgag acgcgcaccc agcccgcgat tccattcgcg ctcgccttcg
     4081 ttgccgacga ggagaccggc agcgtctacg gcctgcgcta tctgctcgaa aagcatcgga
     4141 gcctgttccg caaagacgac ctcatcatca ttccggactc gggcaacccc gacgggaccg
     4201 agctcgagat cgccgagaag gggatcttgt gggtgcgctt ccgcacgctc ggcaagcaga
     4261 cccacggttc cacgccggcc acgggcatca acgcccacaa ggcggcggcc tggatgatcg
     4321 tgcggctgga atcgctctac acgaagttca agaagcgcaa tcctctattt catccctcca
     4381 actcgacctt cgaaccgacc aagaaagaat ccaacgtccc caacatcaat accattcccg
     4441 gagaggacgt cttttatttc gactgccgcg tgctgccggc gtacaagttg gacgaagtca
     4501 tccgcgccat caaggccgtg gtcaaggaga cggagcggaa gttcaaggtc aaggtcgagg
     4561 tgacgttccc tcagaaggag tcgcccgcgc cccccacgcc ggcggactcg ccggtggcgg
     4621 tggcgatcac gcgcgcggtc aaggagttgc gcaagaagcg gcccaaggcg atcgggatcg
     4681 gcggcggcac cgtggccaag tatttccgcg aggccggctt cccgtgcgcc gtgtggtcta
     4741 ccatggacga gcgcgcgcac cggcccgacg agcacgtccg cgtttcccac attctcgcgg
     4801 atgcgaaggc gttcgcgcac atcgccctcg cgggcggggc cgcgccgggc gcgaagaaga
     4861 agtagtcttt gacatctata atcgtttagt ttacattgag gcccaacccc gaaccgggtc
     4921 cacaacattg cgaaaggggc attagacatg cagggaattg cattcggtcc gacgattcga
     4981 ctactgcgcc aagccaaggg catcagcctg cgggagatgg cgcgccaact gggggtgtcg
     5041 cccgcgttct tgagccagat cgaagccggg cgtcagcaca agattccgcg tgcccgcatc
     5101 gtgcaggtcg cggagatgct gggtgtgtcg gaggggtatc tcctgggaac cgccaagcag
     5161 gttcacccgg acctcacgaa gttcttgagc gagacgcccg aggcggccga gttcatggtg
     5221 accgccatgc gcagcggcat gatcgcgcaa gacttcacca acttgcgaga gatcgtgtcc
     5281 gacaagaagc tccgcgccag cgtgctgaag ccggtcgcgg cgcccaaaaa gaaatccggt
     5341 ccgagccata cactcgacgg ctacctggac gcgtcgctgt gcctggccgg ggtcgcggtg
     5401 cagaacaagg agcgcctgtt ccagcgcttc gtcaaggcgg tctcgaagaa gagcaagggc
     5461 atctccggcg aggtgctgct cgatcgcctg atggcgcgcg agcgccaggc gccgaccagc
     5521 atcgggggcg gggtggcgat tccgcacgcg ttcttgcagg gcatcgagaa accgctggtg
     5581 ggcgcgtttc tcctccagcg cgcgattccg tacggaccga cggagagcga ccgggtcaag
     5641 acggtcttct tcctggtcgg ccgcgacggc aacaccgagc accacctgcc gattctcgcg
     5701 cgcatcgcac gcctgtgctc gacgcccgag ttcctgcagg tgctcgccaa agcgaagaac
     5761 ggccgcgaca tcgagcggac gattctggcc tgggacgccc gcatcggctc cgcgtaagga
     5821 gcgcccagta gtcatcgatc gctgaaacgg cccgcgattc gtcgcgggcc gcttcgtttt
     5881 ccaggcgacg ctgcaagcgt cgaccccgcc ggcctttgcg attgcggagc gggccgcatc
     5941 gctggtatca ttgaggcccg aatcgaccta gcatccgcat taccgggac
//
LOCUS       DASPGK010000043         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_361706, whole genome shotgun sequence.
ACCESSION   DASPGK010000043 DASPGK010000000
VERSION     DASPGK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361706"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VEC56_01090"
     CDS             <1..367
                     /locus_tag="VEC56_01090"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXV12776.1"
                     /translation="GGIAFDMYGADLSHHGEDCTFETMIKRFRLADDRALRRLAEIVH
                     DIDLGDSKFDRAEAAGLGAIVRGLTEGIDDDRQRIARVLPVFDGLYRLFGSASNAVEE
                     AKDGKRPRKRARSKAPRRK"
     gene            315..1718
                     /gene="chrA"
                     /locus_tag="VEC56_01095"
     CDS             315..1718
                     /gene="chrA"
                     /locus_tag="VEC56_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXV12777.1"
                     /translation="MESAPGSARVAKHRDASDRASPRGVPFAQALRFWVKLGFISFGG
                     PAGQIAIMHRELVDRRRWVSERRFLHALNYCMLLPGPEAQQLATYIGWLLHRTLGGLV
                     AGAFFVIPSIFVLLALSYVYAAYGSVAAVADVFRGFKPVVAALVVEAVFRIGRRALKG
                     PVHVAIAAAAFVAIHLLHLPFPGVVLGAGLVGFLCARFEPRRIISPLGSSPAPPAAEN
                     ETGGSPADSAVVVDDDALPLAHEVPSGRRALGVLAVGLALWLVPILALAAWQGRESLF
                     VQEYRFFTTAALVTFGGAYSVLSYVTQAAVEQFGWITQAQAIDGLALAETTPGPLIMV
                     LQFVGFMAGWNHPVNVGQTASAVVAALVATYTTFLPSFVFIFLGAPYVERARRNETLG
                     AALAGVTAAVVGVIASLALVFGYVVLWPRGWDGGVDGFAVLLGVAAFVALHRFRVNVL
                     WVVLAAGAAGFAHGLLA"
     gene            1730..>2270
                     /gene="pepE"
                     /locus_tag="VEC56_01100"
     CDS             1730..>2270
                     /gene="pepE"
                     /locus_tag="VEC56_01100"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HXV12778.1"
                     /translation="MEMLASNRTRRLLLISNSTLHGSGYLDHAEGEIRDFLGRTKRVF
                     FVPFALYDRDAYAKLARERFAAMGYGLDSAHEVSDARQAAAEAEVVFIGGGNTFRLLK
                     ALYDHDLVSTIRRRVESGMPYIGSSAGSNVACPTIKTTKDMPIVQPPSFEALGLVGFQ
                     ISPHYLDPDPHSTHMGETQE"
BASE COUNT          339 a          735 c          764 g          432 t
ORIGIN      
        1 gggtggaatc gcattcgaca tgtacggggc ggatctcagc catcacgggg aagactgcac
       61 gttcgagacc atgatcaagc gtttccgtct ggccgacgac cgggccctgc gccggttggc
      121 ggagatcgtc cacgatatcg atctcgggga ctccaagttc gatcgggccg aagcggcggg
      181 gttgggcgcg atcgttcgtg gtctgaccga aggaatcgac gacgaccgcc agcgcatcgc
      241 gcgggttctc cccgttttcg atggtttgta ccggctgttc ggcagcgcat cgaacgcggt
      301 cgaggaggcg aaagatggaa agcgcccccg gaagcgcgcg cgtagcaaag caccgcgacg
      361 caagtgatcg agcgagcccg cggggggttc ccttcgccca ggcgttgcgc ttctgggtca
      421 aactggggtt catctcgttc ggcggccccg ccggccagat cgcgatcatg catcgcgagc
      481 tggtcgaccg gcggcgctgg gtgagcgagc gccgattcct gcacgcgctc aactactgca
      541 tgctgctgcc cggcccggag gcgcagcaac tcgcgacgta tatcggctgg ctgctccacc
      601 gcaccctcgg cgggctcgtc gcgggagcct tcttcgtcat cccttccatc ttcgtgctgc
      661 tcgcgctctc ttatgtctat gcggcctatg gaagtgtcgc cgcggtcgcc gacgtgttcc
      721 ggggcttcaa acccgtggtg gcggcgttgg tcgtcgaagc cgtcttcagg atcggaaggc
      781 gcgcgctcaa ggggccggtg cacgtcgcca tcgccgcggc cgcgttcgtc gccatccatc
      841 tcttgcacct cccgttcccg ggtgtggtac tgggggcggg actggtcggg tttctgtgcg
      901 cgcgcttcga gccgcgacga atcatttctc ccctcggttc gtcccccgcg cccccggctg
      961 ccgagaacga aaccggcggt tccccggccg actccgcggt cgtcgtcgac gacgacgcgt
     1021 tgccgctcgc gcacgaggtg ccgtcgggac ggcgcgcact gggagtgttg gccgtgggac
     1081 tcgcgctgtg gctggtaccg atcctggccc tggctgcatg gcagggacga gaaagtctct
     1141 ttgttcagga gtatcgcttc ttcacgacgg cggctctggt caccttcggc ggtgcctatt
     1201 ccgtgctctc ctacgtgacg caagcggcgg tggagcagtt cggatggatc acgcaagcgc
     1261 aagcgatcga cggcttggcg ctggcggaaa ccactcccgg gccgctcatc atggtcctgc
     1321 agttcgtcgg gttcatggcg ggatggaacc accccgtgaa cgtgggtcag acagcgagtg
     1381 cagtggtcgc cgcactggtg gcaacgtaca ccacgttcct cccctccttc gttttcatct
     1441 ttctcggcgc gccctatgtc gagcgggctc gccggaacga gaccctgggg gccgcactgg
     1501 ccggcgtaac cgcggctgtg gtgggcgtca tcgccagctt ggcgctggtg tttggctacg
     1561 tcgtgctctg gccgcgggga tgggacggcg gcgtcgatgg gttcgccgtg ctgctcggcg
     1621 tcgcggcctt cgtagcactg catcgattca gagtcaacgt gttgtgggtc gtgctcgccg
     1681 cgggcgcggc cggatttgcg cacgggcttc tcgcttgaac aacggaggta tggaaatgct
     1741 cgcaagcaat cgaaccaggc gactacttct gatcagcaac tcgacgctcc acgggagcgg
     1801 ctacctcgac cacgcggaag gggagattcg cgatttcctg ggccgcacga agcgcgtgtt
     1861 cttcgtcccc ttcgccctgt acgatcgcga cgcctacgcc aagctcgcgc gtgagcggtt
     1921 cgccgccatg ggatacggcc tcgactcggc ccacgaggtc agcgacgccc gccaagcggc
     1981 cgccgaagcc gaggtcgttt tcatcggggg cgggaacacc tttcggctgc tgaaggcgct
     2041 ctacgaccac gacctcgtgt cgaccattcg gcgccgggtg gagagcggca tgccgtacat
     2101 cggttccagc gcgggatcga acgtggcgtg ccccaccatc aagacgacga aagacatgcc
     2161 gatcgtgcag ccgccgtcgt tcgaggcgct tggcctggtc ggcttccaga tcagtccgca
     2221 ttacctcgat cccgatcccc actcgaccca catgggcgag acacaggagg
//
LOCUS       DASPGK010000044         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_361787, whole genome shotgun sequence.
ACCESSION   DASPGK010000044 DASPGK010000000
VERSION     DASPGK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361787"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VEC56_01105"
     CDS             complement(<1..342)
                     /locus_tag="VEC56_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12779.1"
                     /translation="MLSLAPRILVLVTLSVLAGCASAPPNVAGDWVGGIRVGTTWHAA
                     HVTIDHAHGLISGRADVKALGVVDAPLEGSLDGRRVRFRVAAGELNELWFEGREEEDA
                     LRSDVHEWRGPA"
     gene            complement(518..1603)
                     /locus_tag="VEC56_01110"
     CDS             complement(518..1603)
                     /locus_tag="VEC56_01110"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV12780.1"
                     /translation="MGVSISADIERRLLGLSRLVGNTPLLALEFRFRGERRVIYAKAE
                     NLNMTGSIKDRMALHIIRRAYETGRLAPGDSIIEATSGNTGIAFAGIGRAMGHPVHIF
                     MPDWMSVERINLIRAYGAEIHLVTREQGGFRGSIEMAEALAESIANGFLPSQFSNPHN
                     VEAHGLGTGPEIWFQLEAHALRPDALVAGVGTGGTIMGAGRYLKHHLPTVKVHPLEPA
                     NSPTLSTGHRVGTHRIQGISDEFIPPIVDLAELDEVVSVDDGDAIIMARKLASEAGLG
                     VGISSGANFLGALKIQNEMGANAVVVTVLPDDNKKYLSTDLLRDEPARDGFLSTNVQL
                     LGFRAFNRVCNICFDAKEQTQTDPLGP"
     gene            1842..3335
                     /locus_tag="VEC56_01115"
     CDS             1842..3335
                     /locus_tag="VEC56_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXV12781.1"
                     /translation="MTSHPATLERKLGLTDATMLVVGSMIGSGIFIVSAESARLVGSP
                     GFLLLAWALAGVMTIAGALCCAELAAAIPRAGGQYVFLREAYGPATGFVFGWSFLLVI
                     QTGTIAAVAVAFANFSGILIPSISSTSYLVEPIRIGHCAISFSTQQATAIAMILLLTL
                     LNARGLVLGKWIQNTFTTAKTAALLGLIVACLALGWKLRGAAFTSSWWNPTDNGWTAQ
                     VAQPGLGVVGALALVMLLGKAMVGPLFSQSAWNNVTFIGAEVRDPGRNLPRALLIGCS
                     LVVGLYLLANLGYVVALSLDQIQRAPQNRVGTAAMQAVLGDRGTVLMAAAIMVSTFGC
                     ANGLILSGARISYAMARDGLFFARAGRVNRYHVPGAALVGQGVWASLLTLPRTAREDA
                     AGSVVYGNVYTQLLEYLVPADLVLYALMVAAVIVLRRKMPAMDRPYRTWGYPIVPAVY
                     IGLALFLVVDLLYVAPATSGVGYAIVLAGFPVYFLWRRVTVRTADAP"
     gene            3344..>3719
                     /locus_tag="VEC56_01120"
     CDS             3344..>3719
                     /locus_tag="VEC56_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXV12782.1"
                     /translation="MGDITRFQPASDQSLMVYFGDRISPDAHRRVRTLLHLLQSEPIT
                     GVRNLHPGYCSILVTFDALSLQHGDLEAKLRACLTRLDGIQLPDPRQVEIPVCYGGDF
                     GPDLDELAASHNMATERAIELHV"
BASE COUNT          574 a         1216 c         1219 g          710 t
ORIGIN      
        1 cgcgggtcct cgccactcgt ggacgtcgct gcgcagcgcg tcttcttctt cgcgcccctc
       61 gaaccacaac tcgttcaatt ccccagcggc cacgcgaaag cgaacgcggc gtccgtcgag
      121 cgacccttcc agcggcgcgt cgacgacgcc gagcgccttg acgtcggcgc gccccgagat
      181 caaaccatgc gcgtggtcga tggtcacgtg cgccgcgtgc cacgtcgtgc cgacccgaat
      241 tcctcccacc cagtccccgg cgacgttggg cggggccgaa gcgcacccgg cgaggacgga
      301 cagagtaacc aggacgagga ttcgcggggc gagcgatagc atggcggcct ccttgggtcg
      361 gtggttcggg cgccggcggc gcagactcac cgtcccacta cggacgggcc ccggcgcggg
      421 ttccgctcgc gctcagaata ccgatccttg atccaggaag aaagctaccg gctggcaaga
      481 tggaactccg tttcgcgcac ctccggaaaa ccttgaccta cggtcccagc ggatccgtct
      541 gcgtctgctc cttggcgtcg aagcagatgt tgcatacgcg gttgaaagcg cgaaagccca
      601 gcagttggac gttcgtcgac aggaatccgt cgcgcgccgg ctcgtcgcgc aacaggtcgg
      661 tgctcaagta cttcttgttg tcgtcgggga ggacggtcac gacgaccgcg ttggctccca
      721 tctcgttctg aatcttcaag gcgcccagga aattggcgcc cgatgagatc cccactccca
      781 gtccggcttc gctcgcgagc ttgcgcgcca taatgatggc gtcgccgtcg tcgacgctta
      841 ccacctcgtc gagttccgcg aggtcgacga tgggcgggat gaattcgtcg gagatgccct
      901 ggatgcggtg cgtgccgacg cggtggccgg tggaaagcgt gggcgagttc gcgggttcga
      961 gtggatgcac ctttacagtg ggcaagtggt gcttcaaata gcggccggcg cccatgatgg
     1021 tcccgcccgt gccgacgccg gcgaccaacg catcgggccg aagcgcgtgc gcttcgagct
     1081 ggaaccaaat ctccggtccc gtgccgagcc cgtgtgcctc cacgttgtgc gggttggaaa
     1141 actgactggg caagaacccg ttcgcgatcg attcggccag cgcctccgcc atctcgatgc
     1201 tgccgcggaa accgccttgc tcgcgcgtga cgagatgaat ctcggcgccg tacgcgcgaa
     1261 tcagattgat gcgttcgacg ctcatccagt cgggcatgaa gatgtggacg ggatgaccca
     1321 tcgcgcgtcc gatgcccgcg aacgcgatgc cggtgttacc gctggtggct tcgatgatgg
     1381 agtcgccggg ggccaggcgt ccggtttcgt acgcccgccg gatgatgtgg agcgccatcc
     1441 ggtccttgat gctcccggtc atgttgagat tttccgcttt cgcgtaaatg acgcgtcgct
     1501 ccccacgaaa gcgaaattcg agcgccagca gaggcgtgtt gcccacgagc cggctgagcc
     1561 caagaagacg tcgttctata tccgcgctga tgcttacacc catgtttcct ctccagtgcg
     1621 gccgccacca tcatagcacc gaacgcgtct caagggcacg atggccgatt cggagtttcc
     1681 gttccggcga cctacttcct cggacgacct gataccgccg cagcgactcc gaaaaccgag
     1741 tcgaaccacg gagcccggct tgcgcgcacc gaccaggcga cgcgctcgat ggccgcggag
     1801 taccatcgcg acgctgccgt atgctaccct cggcgccgcg aatgacctcc catcctgcaa
     1861 ctctcgagcg caaactcgga ctgaccgacg ccaccatgct ggtcgtcggc tcgatgattg
     1921 gctccggcat cttcatcgtc tccgcggagt cggcgcggct ggtggggtcg ccgggctttc
     1981 tgctcctcgc gtgggcgctg gccggtgtaa tgacgatcgc gggtgcgttg tgctgcgccg
     2041 agctggcggc ggcgataccg cgcgcgggtg ggcagtacgt attcctgcgc gaagcctatg
     2101 ggcctgcaac cggcttcgtg ttcgggtggt cgttcttgct ggtgattcag actgggacca
     2161 tcgcggcggt ggcggtggcg ttcgcgaact tctccggaat actgattccg tccatctcgt
     2221 ccacgagtta tctcgtcgaa ccgattcgca tcgggcattg cgcgatcagt ttctccaccc
     2281 agcaagcgac tgcgatcgcg atgatcctct tgctgacact cctcaacgcg cgcggactgg
     2341 tcctcggcaa atggatccag aacacgttca cgaccgcgaa gacggcggcg ctcttgggct
     2401 tgatcgtggc gtgcctcgcg ctgggttgga agctgcgcgg tgccgcgttc acgtcgtcgt
     2461 ggtggaatcc aaccgacaat gggtggaccg cgcaggtggc gcagccgggc ctgggcgtcg
     2521 tgggggctct ggcgctcgtc atgctgctcg gcaaggcgat ggtgggcccg ttgttctcgc
     2581 agtcggcgtg gaacaacgtg accttcatcg gtgccgaagt gcgcgacccg ggtcgcaacc
     2641 tcccgcgcgc gctccttata ggctgctcgc tcgtggtcgg gttgtacctg ctcgccaact
     2701 tgggctacgt cgtcgcactg tcgctcgacc agatccaacg cgcgccgcag aatcgcgtcg
     2761 gcacggcggc gatgcaggcg gtcctgggcg atcgcggcac ggtgctgatg gcggcggcca
     2821 tcatggtgtc gaccttcggg tgcgccaacg ggctcattct ctccggcgcg cgcatctcgt
     2881 acgcgatggc gcgcgacggg ctgttcttcg cgcgcgcggg ccgggtcaac cgctaccacg
     2941 tgccgggtgc ggccctggtt gggcaggggg tgtgggcctc actgctgaca ctcccgcgca
     3001 cggcgaggga agacgccgcg ggtagcgtcg tctacgggaa tgtctacacg caactcttgg
     3061 agtatttggt cccggccgac ttggtgctgt acgctctgat ggtggcggcc gtgattgtgc
     3121 ttcgtcgcaa gatgcctgcg atggatcggc cgtatcggac gtggggctat ccgatcgtgc
     3181 ccgcggtgta catcggtctc gcgttgttcc tcgtcgtgga cttgttgtac gtcgcgccgg
     3241 ccacgtctgg cgtgggatat gcaatcgtgt tggcgggatt ccccgtgtat ttcctatggc
     3301 gccgcgtgac ggtgcgaacg gcggacgcgc catgaggtga gcgatgggcg acatcacgcg
     3361 ctttcagccg gcgagcgacc agtcgctcat ggtctacttc ggcgaccgca tctcgccgga
     3421 cgcccatcga cgcgtgcgca cgctcctgca cctcctccaa tccgaaccga tcaccggcgt
     3481 ccgcaatctc caccccggct actgctcgat cctcgtcacc ttcgacgcgc tctcgctgca
     3541 gcacggcgac ctcgaggcga agctgcgcgc gtgcctcacg cgcctggacg gtatacagct
     3601 gccggacccg cgccaggtcg aaattcccgt gtgctacggc ggtgacttcg gccccgatct
     3661 cgacgagctg gcggcgtcgc acaacatggc gaccgaacgc gcgatcgagc tgcacgtat
//
LOCUS       DASPGK010000045         7925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_713356, whole genome shotgun sequence.
ACCESSION   DASPGK010000045 DASPGK010000000
VERSION     DASPGK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713356"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2543
                     /locus_tag="VEC56_01125"
     CDS             303..2543
                     /locus_tag="VEC56_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXV12783.1"
                     /translation="MTLEIRLHGRGGQGGVTCAKILAAIYARLGKSVQTFGDYAGERS
                     GAPVRAYTRVADQPITNRNKVYRPDHLLVLDPTLLSAEVVSGLAPGGLLLINTPDPPA
                     AFAEHFAAFRVATIDATAIARRHGIGTRSVVIVNTTIAGAYCKIVGLPYDALEFAYRD
                     LHLHGNLPAAKEAYDAVLAAEFPAALPARAAGTAHADAPASLTHGTPVMPLTEHTHSL
                     PTQLKTGSWRTQLPRYVEHLAPCNASCPAGNDVVTFVQTLMNEGEAAAADVLGRTQPL
                     ASVCGRVCPAPCMVGCSRSNYDGAVNIRALERWIGDRAVPVQRKERPPAKQRRIAVVG
                     SGPAGLSAAYELARGGHAVTIYDGEKELGGVLRTGIPTYRLPRDVLDREIAGILKLGV
                     QTRMGEFLPRDRILALAGEYDAVILAAGFGRPTKLEVPGVALAGVEDGTRFLHRVNLE
                     GGEAVRGHVVVLGGGNTAMDCARSALRSGASRVTVAYRRTRTEMPAIIEEIEESEHEG
                     VVFQFQRAPVAIRGNGRVAALVTADVEMGPPDASGRRRPVVTDRTSVMTCDHVLLALG
                     QSADMGLLPEGATIEDGRVHVGGEPTNLFASGDFATGEGTVAHAIGDGRRAAARSLSS
                     LGEDVAVFARPKKTQAVPLSSIRMEYFEPRLAATERHEPVATRITTFAETNHGIPDPR
                     EAERCFSCGHCTFCDTCLVYCPEGIVDRVISKTGNGYEIDLDYCKGCGICVSECPRGA
                     MEMFPQ"
     gene            2540..3703
                     /gene="porA"
                     /locus_tag="VEC56_01130"
     CDS             2540..3703
                     /gene="porA"
                     /locus_tag="VEC56_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HXV12784.1"
                     /translation="MSQQLISANHAAAEAALLAARANRRGRGFCSGVYPITPQTECIE
                     RLCSVPIEKGDVVRVESEHSAMGVCIGGSLSGARTFTASSSNGLAYMAENVFAAGFFR
                     LPIVMMAVNRTLGPPWNIWVDHGDTLMLRDSGWIQLYCEDNQEVFDTTLLAYRLAEDA
                     RVYLPVMVCQDAFVLSHTMMMTDIPAQEQVDRFLPVLDLSFRVSDKPRVVGGLDFPHQ
                     TEMHREQHIEAMKQVPAVYAEIQDEFEQAFGRRPPDAVVPYRMDDAEIAIVSMGTTAS
                     TVRTAVDGARARGIKAGGLRVRLFRPLPEDSIATALRACPRVAVIDRNLSPGLGGILW
                     GEIRGLARPNAIVQNYLVGLGGGDIRPEHVASIIQDVTERKRAGEPVFMEAGE"
     gene            3700..4641
                     /locus_tag="VEC56_01135"
     CDS             3700..4641
                     /locus_tag="VEC56_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV12785.1"
                     /translation="MKAEPILGGIANTAADRCEPLLRAGNTNCGGCGMSIALNMISRA
                     IAERPVQMVIPACCGIVTAGSFPFSAYGAPVVASTFAGAAAVATGLARVARANGETTR
                     VLCFAGDGGTYDIGMATVSAAAERDEDILYVCYDNEIYGNTGGQRSSATPLGASTSTT
                     PRGKHHEKKDILSIMASHRIPYAASVSLAHPEDTMRKFRRALDARGFRFLHILSPCPT
                     GWKSEPAEGIELIRQAVDSGLYPVIEIVDGTEYTINVEPPFSVDALRAFIEGQGRFVK
                     SKISVEEVLVGVRRQWEVLRRHVRVQGAWGASEEIDD"
     gene            complement(4743..6935)
                     /locus_tag="VEC56_01140"
     CDS             complement(4743..6935)
                     /locus_tag="VEC56_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HXV12786.1"
                     /translation="MRKSVVVCLLAINAILAFSTVYAVPVHQWSTCFGGPGNNTAYDV
                     AVDGSGNIVIVGTFVGAMTIGGQVLASAGASDIFLAKFDANGVHQWSQRFGGTGNDVG
                     KSVTVDGAGNIVMTGHFRGPVSVGGDPLPGRALQDIVVAKYDGAGLHQWSQWYGSAGG
                     DEGQDVVADASGNLIVTGLYGGVIQFGGGADTPAFGGADMFVLKLDAAGGHQWSKGMG
                     SASTDAGYGIAVDASNRVSITGVFTNTVDFGGGSLTSAGSTDAFVVTYAENGDHLWSA
                     RLGDAATDVGQGIAADAMGGIVVTGTNNGAFLLKYDPNGALEWSQSFRSTVLVQGQDV
                     ALGPGGTIVLTGNLRGTTSFGGASLTSAGDDDAFLAFFEGNGTHRWSQRFGNTGDDWG
                     HGCYFDPSGGLTVTGVFSSSVDFGGGPLVSAGLNDVYVLKFAEEADTSPPVITCPADA
                     AVEQTTPEGTPATHAVIAAFLTGASASDGVDPAPVVTHDAPDTFPVGTTSVTFRATDA
                     AGNFAECTATVEVVDTTPPQITLVLDKELLWPPNNQYETVCAKVTVSDNGTGEPTFWL
                     VSITSDEPVERGPRQRAQDISGAEFGTPDLCFELRAKRAGNGDGRVYTITYATIDGSG
                     NTVHATAFVRVPHDMSATLTSVHPNPFNPQTTLEYTLSVGDRVQIAIYDVRGSLVRRL
                     VNQVMPAGEHRVTWNGLDQAGRPAGSGIYFVKLSAGSDVDTRKIVLLK"
     gene            complement(7224..7547)
                     /locus_tag="VEC56_01145"
     CDS             complement(7224..7547)
                     /locus_tag="VEC56_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12787.1"
                     /translation="MNSRLNELAERRQALLARSDQNRAGIASIFDGLERKLAVAEVLV
                     ATARGLSRYRALVGAAGVFMVFAPRAFRSRLRRAMWLVPLVVGGYRAVKAHGEARDDS
                     PLNDD"
     gene            complement(7544..>7925)
                     /locus_tag="VEC56_01150"
     CDS             complement(7544..>7925)
                     /locus_tag="VEC56_01150"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXV12788.1"
                     /translation="NAEPPRGIFDSLRALFDTALSIVHNRAELFTTEIEEEVTRLVGV
                     ALWAFAAVLAGIIGTAFVGVAVLMVLPAGYRVWVAGGLGLLLLLFAGIGFVAIRRIAR
                     MKPRPFDASLRELEKDREHLRGRT"
BASE COUNT         1354 a         2705 c         2572 g         1294 t
ORIGIN      
        1 ttgacgcccg cccccgttcc tacccgcgag acgacgtggg gacagatcaa ggcgttctat
       61 cggtaacctc tgttaagggt taaggaccga ccgtcgcaac ggccatcctc gctctacgag
      121 ggtggccggt gtgtttttgg gagaaatccc aactcgtttt accacaacgt gttggatccg
      181 gagcgaagcg ccgtcggcgg tgagatttcc gttgtcccga ccatcgaatc tagatacaat
      241 atcagagtaa gatatctgct tatctactac gatgagcggg taccgaggag ggcgatcacc
      301 gcatgacact cgaaattcgg cttcatgggc gaggcggtca aggtggtgtt acctgcgcca
      361 agatcctcgc ggccatttac gcacgccttg gaaagagcgt gcagaccttc ggtgactacg
      421 ccggcgaacg ctccggggca ccggttcgcg cgtacacgcg ggtcgccgac caacccatca
      481 ccaaccgcaa caaggtctat cgcccggatc atctgctcgt actcgatccc acgttgttga
      541 gcgccgaggt cgtctccggc ctggcaccgg gcgggctgtt gctcatcaac actcccgacc
      601 cgcccgcggc attcgcggag cacttcgccg cgttccgggt ggccaccatc gacgccaccg
      661 ccatcgcgcg ccgacacggg atcgggaccc ggtcggtggt gatcgtcaac acgaccatcg
      721 cgggcgcgta ctgcaaaatc gtcggacttc cctacgacgc gctcgagttc gcgtaccgag
      781 acctgcatct ccatggcaac ctgcctgctg ccaaggaggc gtacgacgcc gtgctggccg
      841 ccgaattccc cgcggccctg cctgcccgcg cggccggcac ggcgcacgcc gacgcaccgg
      901 cgtcgctcac ccacggcact cccgtcatgc cgctgaccga gcacacgcac agcctaccca
      961 cccagttgaa gacgggatcg tggcgcacac agctgccgcg ttacgtggaa cacctcgcac
     1021 cgtgcaacgc ctcctgcccg gccggcaacg acgtggtgac cttcgtgcag acgctaatga
     1081 acgagggcga agcagccgcg gccgacgtac tcggtcgcac ccagccgctc gcgtcggtgt
     1141 gcggacgcgt gtgccccgcg ccctgcatgg tcggctgcag ccgttccaac tacgacggcg
     1201 ccgtcaacat ccgcgccctc gagcgctgga tcggcgaccg cgcggtgccc gtgcagcgca
     1261 aagagcggcc tcccgccaag cagcgtcgca tcgccgtcgt cggcagcgga cccgcggggc
     1321 tttccgcggc ctacgagctc gcgcgcggcg gtcacgccgt cacgatctac gacggagaga
     1381 aggagctcgg cggcgtgttg cgcaccggga ttcccaccta tcgcctcccg cgcgacgtgc
     1441 tcgaccgcga gatcgccggg atcctcaagc tcggtgtgca aacgcgcatg ggcgagtttc
     1501 tccctcgcga tcgcatcctc gcgctcgcgg gcgagtacga cgcggtcatt ttggccgccg
     1561 ggttcggccg gccgaccaag ctcgaggtgc cgggcgtcgc cttggcgggc gtcgaagacg
     1621 ggacgcgttt cttgcaccgc gtcaacctgg agggcggcga agcggtgcgc gggcacgtgg
     1681 tcgttctcgg cggcggaaac acggcgatgg actgcgcacg cagcgcgctt cgctcgggtg
     1741 cctcgcgcgt gacggtggcg tatcgacgca cgcgcaccga aatgcccgcg atcatcgaag
     1801 aaatcgagga gagcgagcac gagggtgtgg tgttccagtt ccagcgcgcc ccggtcgcca
     1861 tccggggcaa cgggcgggtc gcggcactcg tcaccgcgga cgtcgagatg ggtccgcccg
     1921 acgcgagcgg gcgccgccgc ccggtcgtga ccgatcgcac gtcggtgatg acgtgcgacc
     1981 acgtgctcct cgccctcggc cagtccgcgg acatggggct cctgcccgag ggcgcgacca
     2041 tcgaagacgg acgcgtgcac gtcggcggcg agccgaccaa cctcttcgcc tcgggcgact
     2101 tcgccaccgg cgagggaacc gtggcccacg ccatcggcga cggccgtcgt gccgcggcgc
     2161 gctctctctc gagcctcggc gaggacgtgg cggtcttcgc gcggcccaag aagacgcagg
     2221 ccgtgccgct gtcgagcatt cgcatggagt acttcgagcc gcgcctggcg gcgaccgagc
     2281 ggcacgagcc ggtggcgaca cgcatcacga ccttcgccga gaccaaccac ggcatccccg
     2341 atccgcgcga ggccgagcgc tgcttctcgt gcgggcattg caccttctgc gacacctgcc
     2401 tggtgtactg ccccgaaggc atcgtcgacc gcgtgatctc caagaccggc aacggttacg
     2461 aaatcgacct cgactactgc aagggctgcg gtatctgcgt gtccgagtgc ccgcgcggtg
     2521 cgatggagat gtttccccaa tgagccaaca actgatcagt gccaaccacg ccgcggcgga
     2581 ggccgcactg cttgccgccc gcgccaatcg ccgcgggcgc ggcttctgca gcggcgtgta
     2641 ccccatcacg ccgcaaaccg agtgcatcga gcgcttgtgc agcgtgccga tcgagaaagg
     2701 cgacgtggtg cgcgtggaga gcgagcacag tgccatgggc gtgtgcatcg gcgggtcgct
     2761 ctcgggcgcg cgcacgttca cggcgtcgtc gtccaacgga ctcgcataca tggccgagaa
     2821 cgtgttcgcc gcgggtttct tccgccttcc catcgtgatg atggcggtca atcgcacgct
     2881 cggtccgccg tggaacatct gggtggacca cggcgatacc ttgatgctgc gcgattcggg
     2941 ctggattcag ctctactgcg aagacaacca ggaagtgttc gacaccacgc tgctcgcgta
     3001 ccgtctggcg gaggacgcgc gcgtctatct ccccgtgatg gtgtgccagg acgcgttcgt
     3061 gctctcgcac accatgatga tgaccgacat ccctgcgcag gagcaggtgg accggttcct
     3121 tcccgtgctc gacctctcgt tccgcgtgag cgacaagccg cgggtggtgg gaggcctgga
     3181 cttcccccac caaacggaga tgcaccggga acagcacatc gaggccatga agcaggtgcc
     3241 cgcggtctac gccgagatac aggacgaatt cgagcaggcg ttcggccgcc gtccccccga
     3301 cgccgtcgtg ccctaccgca tggacgacgc cgagatcgcg atcgtcagca tggggacgac
     3361 cgcgagcacc gtgcgtaccg ccgtcgatgg ggcacgcgcg cgcggtatca aagcgggggg
     3421 gttgcgcgtg cgtctgttcc gccctctgcc cgaagactcg atcgcgaccg cgctgcgcgc
     3481 gtgcccgcgc gtcgcggtga tcgatcgcaa tctgtccccc ggcttgggcg gcatcctgtg
     3541 gggcgagatt cgcggcctcg cgcgcccgaa cgcgatcgta cagaactact tggtgggact
     3601 cggcggcggc gacatccggc cggagcacgt cgcgtcgatc atccaggacg tgacggagcg
     3661 gaaaagagcc ggcgagccgg tcttcatgga ggcgggcgaa tgaaagccga accgatcctg
     3721 ggcggcatcg ccaacaccgc ggcggatcgc tgcgagccct tgctgcgcgc gggcaacacc
     3781 aactgcggcg gctgcggaat gtcgatcgcc ctcaacatga tcagccgcgc catcgcggag
     3841 cggccggtgc agatggtgat tcccgcctgc tgcgggatcg tgaccgcggg gtcgttcccg
     3901 ttcagcgcct acggcgcgcc ggtggtggcc tcgacgttcg cgggggccgc ggcggtagcg
     3961 accgggctcg cgcgcgtggc acgcgccaac ggcgagacga cgcgcgtgct gtgcttcgcc
     4021 ggtgacggcg gcacctacga catcggcatg gccacggtga gtgcggcggc cgagcgcgac
     4081 gaggacattc tgtacgtctg ttacgacaac gagatctacg gcaacaccgg aggtcaacgc
     4141 tcgtcggcca caccgttggg cgcatcgacg tccaccacgc cgcgcggcaa gcaccacgag
     4201 aagaaggaca ttctgtccat catggcgtcg caccgaattc cctacgcggc ctccgtgtcc
     4261 ctcgcgcacc ccgaagacac gatgcgcaag ttccgtcgcg cgctcgacgc gcgcgggttc
     4321 cgtttcctgc acattctctc cccgtgcccg acggggtgga agtcggagcc ggccgagggc
     4381 atcgagctga tccgccaggc ggtcgactcg ggcctctatc ccgtcatcga gatcgtcgac
     4441 ggcaccgagt acacgatcaa cgtcgagcct ccgttttcgg tggacgcgct gcgcgcgttc
     4501 atcgagggac aggggcgctt cgtgaagtcg aagatctcgg tggaagaggt cttggtcggg
     4561 gtgcgccgcc agtgggaagt ccttcgccgc cacgtgcggg ttcagggagc ctggggagcc
     4621 tcggaggaaa tcgacgacta gtcgtacgcg atccgggcga agcgtacggc ctgggaggcc
     4681 gacgcttcgt ccggttggca cgcgccaggg agcggatttc gtgaccggtt gatacgggcg
     4741 ggttacttca gcagcacgat cttgcgcgta tccacgtcgg aaccggcgga cagcttcaca
     4801 aaatagattc cggaaccggc cggccggccg gcctggtcga gtccgttcca cgtcacgcga
     4861 tgctcacccg cgggcatgac ctgattcacc aggcggcgta ccaacgaacc gcgcacgtcg
     4921 tagatcgcga tctgcacccg atcgcccacc gacagcgtgt actccagcgt cgtctgtggg
     4981 ttgaagggat tcggatgtac agatgtcagc gtagccgaca tgtcgtgcgg cacgcgaacg
     5041 aacgccgtag cgtggaccgt gttgcccgag ccgtcgatgg tggcatacgt gatggtgtag
     5101 acccggccgt cgccgtttcc agcgcgtttc gcgcgcaact cgaagcacag gtcgggagtt
     5161 ccgaactcgg cgcccgagat gtcttgcgcc cgttgacggg gtccgcgctc taccggctca
     5221 tcggacgtga tggaaaccaa ccagaacgtc ggctccccgg tgccgttgtc cgacacggtc
     5281 accttcgcgc acaccgtctc atactggtta ttggggggcc acagcagctc cttgtccaag
     5341 accagcgtga tctgcggggg cgtcgtgtcg actacctcga ccgtggccgt gcattcggcg
     5401 aagttgcccg ccgcgtccgt ggcgcggaac gttaccgacg tcgttccgac cggaaaagtg
     5461 tcgggcgcgt cgtgcgtcac caccggagcc gggtccacgc catccgacgc cgatgcgccg
     5521 gtcagaaagg ccgcgattac ggcgtgcgtg gcgggggtcc cttccggcgt cgtctgttca
     5581 accgcggcat cggcgggaca ggtgatcacg gggggcgacg tgtccgcttc ctcggcaaac
     5641 ttcagtacat agacgtcgtt caggccggca ctgaccagcg ggccgccccc aaagtcgacc
     5701 gatgaggaga atacgcccgt caccgtcaga ccacccgacg ggtcgaagta acatccgtgg
     5761 ccccagtcat ccccggtatt gccgaagcgc tggctccagc gatgagttcc gttcccttcg
     5821 aagaaggcga ggaacgcgtc gtcatcgccc gcgctggtga gcgaggcgcc gccgaagctc
     5881 gtggtaccgc gcagattgcc ggtaagcacg atggtgccgc cggggccgag cgcgacatcc
     5941 tggccctgta ccaggacggt gcttctgaaa ctctggctcc actccagcgc accgttcggg
     6001 tcatacttga gtaggaatgc gccattgttg gtccccgtga cgacgatgcc gcccatcgca
     6061 tccgcggcga taccctgccc cacgtcggtg gccgcgtcgc cgagccgcgc gctccacagg
     6121 tggtcaccgt tctcggcgta cgtcaccacg aatgcatcgg tcgaacccgc gctggtgaga
     6181 gagcccccgc cgaaatcgac cgtgttggtg aatacccctg tgatcgaaac acggttggac
     6241 gcgtccaccg cgatgccgta cccggcgtcg gtactggcgc tgcccatacc cttgctccac
     6301 tgatggcctc ccgccgcgtc cagcttcaga acgaacatgt cggcgccgcc gaacgccggc
     6361 gtatccgcgc ccccgccaaa ctggatgacg ccaccgtaga ggcccgttac gatcaaattg
     6421 cccgacgcat cggcgaccac gtcctgcccc tcgtcgccgc ccgcgcttcc gtaccattga
     6481 ctccactggt ggagaccggc cccgtcgtac ttggccacga cgatgtcctg tagcgcccgg
     6541 cccggcagcg ggtcgccgcc gacgctgacc ggtcctctga agtgccccgt catgacgatg
     6601 ttgcccgcgc cgtccaccgt gacgctcttg ccgacatcgt tacccgtacc accgaacctc
     6661 tggctccact ggtgcacgcc attggcatcg aatttggcga ggaagatgtc cgacgccccg
     6721 gcactcgcga gcacctggcc gccgatggtc atcgcgccga cgaatgtgcc gacgatcacg
     6781 atgttgcccg aaccgtcgac cgcgacatcg taggcggtgt tgttgcccgg cccgccgaag
     6841 caggtactcc attgatggac cggaaccgcg tacacagtag aaaacgcgag aatggcattg
     6901 atcgccagca ggcacacgac cacggattta cgcattgtgg gcacctccgc gcgggtgtag
     6961 cgaaagacga gtcgtaccca gcgccgaagc gagaaagaag ggggagtctg cagaaacagc
     7021 tctgcccgcg cctcaattac tcgttgtttt cacggcgaat cgcgccaggc atccattcct
     7081 tggaacttca agcctcgcgc aggagcactt gtccttcgtg accgcgcaaa tggtggaagg
     7141 gagcatttcc catgccccgg caagacttgc ccggcttgtg caagccggcg tgatgtgtgg
     7201 aattggaact cgccagctcg cggtcagtcg tcgttgagcg gagagtcgtc acgcgcttcg
     7261 ccgtgggcct tcacggcgcg gtatcctccg acgacaagag gcaccagcca catcgcgcgt
     7321 cgcagcctgc ttcggaacgc ccggggggcg aacaccatga acacgccggc ggcaccgacc
     7381 agcgcccgat accggctcaa cccacgcgcg gtggcaacca gcacttccgc gacggccaac
     7441 ttgcgctcga gcccgtcgaa gatcgacgct atgccggcgc ggttctgatc ggatcgagcg
     7501 agcaatgctt gccggcgttc ggccagctcg ttgagccgcg agttcacgtc ctgccccgca
     7561 ggtgctcgcg gtccttctcc aactcgcgca agctggcgtc gaagggccgc ggtttcatgc
     7621 gcgcaatcct acggatggcg acgaatccga ttcccgcgaa caacagaagc aggagtccca
     7681 ggccgcccgc gacccagacg cgataccccg cgggaagcac catcaggacc gcgacgccga
     7741 cgaaggccgt gccgatgatc cccgcgagca ccgcggcgaa ggcccacagg gcgacgccga
     7801 cgaggcgcgt cacctcttcc tcgatttcgg tcgtgaatag ctcggcccga ttgtggacga
     7861 tcgacaacgc cgtatcgaac agcgccctca aggagtcgaa gatccctcgc ggcggttcgg
     7921 cgttc
//
LOCUS       DASPGK010000046         5444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1415735, whole genome shotgun sequence.
ACCESSION   DASPGK010000046 DASPGK010000000
VERSION     DASPGK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415735"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="fabD"
                     /locus_tag="VEC56_01155"
     CDS             <1..897
                     /gene="fabD"
                     /locus_tag="VEC56_01155"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXV12789.1"
                     /translation="VGMLFPGQGSQFVGMGKDYYDAFPEARALYRAANDALGFDIAGL
                     SFSGDMDELTKTKNAQPAILLHSVAVLEVLNARGVAPAVVAGHSLGEFSALVAAGFFR
                     PLDALRIVRRRGELMYDAGVKRPGTMAAIIGLDEPTVEACVAEAGGVVVVANYNSPAQ
                     FAISGDVDAVERACEVCKARGAKRALKLPVSGAFHSPLMEPAVDEFRAFLAGFPAGEL
                     RVPWVANVTGGVVAERERVLDLLAQQLKSAVQWTKSMRAFGAVHAGPSYEVGPGAVLA
                     GLMKRIVDGADVVSLSSASALP"
     gene            1043..1789
                     /gene="fabG"
                     /locus_tag="VEC56_01160"
     CDS             1043..1789
                     /gene="fabG"
                     /locus_tag="VEC56_01160"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXV12790.1"
                     /translation="MDLAGKTVVVTGAARGIGREIAGRLAGAGAALAMVDMAEETVVQ
                     SAAEVARTGATARGYRCDVANFDDVAEVGERIVADFGRVDVLVNNAGITRDKLFLRMS
                     PEDWGQVIAVNLTGAFNFTKALAPAMLKQRGGSIINIASVVGQAGNAGQANYAASKAG
                     LIGLTKSLAREFAPRGVRVNAVAPGFIRTAMTDALTEEQQNQMKQAIPLGRLGEPADV
                     ADVVMFLASNLAGYVTGQVINCDGGMITAR"
     gene            1816..2052
                     /gene="acpP"
                     /locus_tag="VEC56_01165"
     CDS             1816..2052
                     /gene="acpP"
                     /locus_tag="VEC56_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXV12791.1"
                     /translation="MSDIEAKVKKIIEEKLSVNADQITNEARFAEDLKADSLDTVELV
                     MALEDEFGLDIPDEEAEKIKTVQNAIDYITAKAG"
     gene            2132..3385
                     /gene="fabF"
                     /locus_tag="VEC56_01170"
     CDS             2132..3385
                     /gene="fabF"
                     /locus_tag="VEC56_01170"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXV12792.1"
                     /translation="MTRETHPRRVVVTGVGAVTSLGLSTADTWAAIVAGKSGAAPIAS
                     FDASGYTTRIGAEVRHFEPAEFMDLKDSKRTDRVVHLTMAAARQAIAGIDFDRLDRNR
                     VGVVIGSGIGGIATFEKQHANLLQRGPDKVSPFFIPMMISDMTSGYASIALNLRGPNY
                     TTVSACSSGGNAIADAFLLIQAGLADAILTGGAEAAITPMSFAGFCSAKAMSRRNDAP
                     EKASRPFDAERDGFVMGEGAGILFVEELEHARRRGAKILGEIVGVGLTGDAFHMTSPA
                     PNGEGAVRAMQMALEVAHLAPAAVDYINAHGTSTELNDASETAAIKHVFGEHAYKLAV
                     SSTKSMVGHLLGAAAAVELIFCLLAIRDDVIPPTINQEHPDPACDLDYVPNAARKRRI
                     DVALSNAFGFGGHNTAIIVRRYADG"
     gene            3393..4178
                     /gene="rnc"
                     /locus_tag="VEC56_01175"
     CDS             3393..4178
                     /gene="rnc"
                     /locus_tag="VEC56_01175"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXV12793.1"
                     /translation="MTGAFRRLLGRFLGSKRGSGAPAPHTVAHLVDLEQTLDYRFRDR
                     SLLVASLVHRSYYAGTEINAALQSNERMEFLGDSVLSLVVNEHLYKRYPERTEGELTK
                     MKSVVVSKQILAHLAKKMDLGRYVLVSENAQRAGVSTMESVVADALEAVFGAVFIDGG
                     FEAARRVVLGVLPDNLGEVISQEGTINYKSLLQEYIQALHKVPPRYRVHSTTGPDHDK
                     QFAVEVVVKGNILGSGTGKTKKDAEQKAAREAYRRLTNSPAEG"
     gene            4220..4711
                     /locus_tag="VEC56_01180"
     CDS             4220..4711
                     /locus_tag="VEC56_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12794.1"
                     /translation="MGRPEIEVPEAPYTYGGEKVRLCRDCRLPCHLDVTSPDCFRRFF
                     LQGMLQCTYFVYGYCFLVDRQGCVFDNGGDHDQYWLQLLNSYFHNVAGREEDDDEFAR
                     RRAELIHLVSEQIESRQVSLCRYFEEIYNKILEDGGGMSEDVMARLDEAYLSLLSGTS
                     RKR"
     gene            complement(4770..>5444)
                     /locus_tag="VEC56_01185"
     CDS             complement(4770..>5444)
                     /locus_tag="VEC56_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12795.1"
                     /translation="TFVYEDTDADVDGYGARFGLVFYALERAQLAVSLTAPMVVEVEA
                     NVVTETTRQVDNDVGSFTRETTSTTTDYRTPYSIEAAIAVPASRSLLLTAQVGYADWS
                     KATIDDERVITSSLQSVMRKVVDLRAGVEWSVSRWPLRVRAGFAQSRAAADFLEADRI
                     DNDRLERIDHESETRRYSVGLGWMVRGSIVIDAAGAYARGERASPTVSDERDAASLSI
                     GCGYYF"
BASE COUNT          922 a         1777 c         1875 g          870 t
ORIGIN      
        1 gtcggcatgt tgttccccgg acaggggtcc cagttcgtcg gcatggggaa ggactactac
       61 gatgcctttc ccgaagcgcg cgcgttgtat cgcgcggcca acgacgcgct cggcttcgac
      121 atcgccgggc tgtcgttctc cggagacatg gacgagctga ccaagaccaa gaacgcgcag
      181 cccgcgattc tcttgcacag cgtcgccgtg ctcgaggtcc tgaacgcgcg cggtgtggcg
      241 ccggcggtgg tcgcgggaca cagcctggga gagttctccg cgctggtggc ggccggcttc
      301 tttcgcccgc tcgacgcgtt gcggatcgtg cgccggcgcg gcgagctcat gtacgacgcg
      361 ggggtcaagc gccccggcac catggccgcc atcattggat tggacgagcc cacggtggaa
      421 gcgtgcgtgg ccgaagccgg tggggtggtg gtggtcgcga actacaactc gccggcgcaa
      481 ttcgcgattt cgggcgacgt ggacgcagtc gagcgcgcgt gcgaggtctg caaggcgcgc
      541 ggcgccaagc gcgcgctcaa gctcccggtc agcggcgcct tccactcgcc gctgatggag
      601 cccgcggtgg atgagtttcg cgcgttcctg gccggcttcc cggccggcga gctgcgcgta
      661 ccgtgggtgg ccaacgtcac cggcggggtc gtcgcggagc gcgagcgcgt tctcgatctc
      721 ctcgcgcagc agctgaagtc ggcggtgcag tggaccaaga gcatgcgtgc gttcggcgcc
      781 gtgcacgcgg gtcccagtta cgaagtcggg ccgggtgcgg tgctcgcggg cctgatgaag
      841 cgaatcgtcg acggcgcgga cgtggtgtcg ctctcgagcg cctccgcgct cccataatgg
      901 tgcgccggtg gcgcggggcg gcggggtcga cccgccggcc gacgcaactt tctcttttcg
      961 ggccgcgcgg cggtctgcta taagtcgcgg gcgcgtcgga gcggtcgggg gaccggggcc
     1021 ggattcgcaa caacgcacga ccatggacct tgcgggaaag acggtggtgg tgacgggcgc
     1081 ggcgcgcggg atcggccgcg agatcgccgg tcgcctggcg ggcgcgggcg cggcgctcgc
     1141 gatggtcgac atggccgaag aaaccgtcgt gcagtcggcg gcggaggtgg cccgcaccgg
     1201 tgccaccgcg cgcgggtacc gctgcgacgt ggcgaacttc gacgacgtgg cggaggtggg
     1261 cgagcgcatc gtcgccgatt tcgggcgcgt cgatgtgctg gtcaacaacg ccggtatcac
     1321 gcgcgacaaa ttgttcttgc gcatgtcgcc cgaagactgg gggcaggtga tcgccgtcaa
     1381 cctgacgggc gcgttcaatt tcaccaaggc cctcgcgccc gcgatgctca agcagcgggg
     1441 cggcagtatt atcaacatcg cgtcggtggt ggggcaggcg ggaaacgcgg gacaagcgaa
     1501 ctacgccgcc agcaaggcgg gcctgatcgg cttgaccaag tcgctggcga gagagttcgc
     1561 gccgcgcggg gtgcgcgtca acgcggtcgc acccgggttc atccggaccg cgatgacgga
     1621 cgcactgacg gaagagcagc agaaccagat gaagcaagcg attccgctgg gacgcctggg
     1681 cgaacccgcg gacgtcgccg acgtcgtgat gttcctggca tcgaacctgg cggggtatgt
     1741 caccggccag gtcatcaatt gtgacggtgg aatgataacg gcgcgctagc gccgcagcag
     1801 gaggtgaacc tcgacatgtc ggatatcgag gccaaggtca agaaaatcat cgaggagaag
     1861 ttgtccgtga acgcggacca aatcaccaac gaggcccgct tcgccgagga cctcaaggcg
     1921 gactcgctcg acacggtcga gctcgtcatg gccctcgagg acgagttcgg tctcgacatt
     1981 cccgacgagg aagccgagaa gatcaagacc gtccagaacg ccatcgacta catcacggcc
     2041 aaagccggtt agtccgaacg gcggccgcgc gcgcgccgtg atcacaccgc gcgcgccttc
     2101 gaagccgcga ccggctcggc gttttcgatt tatgactcgc gagacccatc cgagaagagt
     2161 cgtcgtcacc ggggtcggcg ccgtcacgtc gctcgggctt tccaccgcgg atacctgggc
     2221 cgcgatcgtc gccggcaaga gcggagccgc gcccatcgcg agcttcgacg cgtccgggta
     2281 cacgacgcgc atcggggccg aggtgcgcca cttcgagccc gcggagttca tggatctcaa
     2341 ggactccaaa cgcaccgacc gcgtcgtgca cctgaccatg gcggcggctc ggcaggcgat
     2401 cgccggcatc gatttcgacc gcctcgaccg caaccgcgtg ggggtcgtga tcgggtcggg
     2461 tatcggtggt atcgcgacct tcgagaagca gcacgcgaat ctcctccagc gcggccccga
     2521 caaagtgagc ccgttcttca ttcccatgat gatctccgac atgacgtcgg ggtacgcgtc
     2581 gatcgcactc aacttgcgcg gacccaatta caccaccgtg tcggcttgct cgtcgggcgg
     2641 caacgccatc gccgacgcgt tcctcttgat ccaagcgggg ctggccgacg ccatcctcac
     2701 cggtggcgcc gaagcggcca tcacgcccat gtcgttcgcc ggcttctgct cggccaaggc
     2761 gatgtcgcga cgcaacgacg cgcccgagaa agcctcgcgc cccttcgacg ccgagcgcga
     2821 cggcttcgtg atgggcgagg gggcgggcat cctcttcgtc gaagagctgg agcacgcgag
     2881 gcgccgcggc gccaagatcc tgggcgagat cgtcggcgtc gggttgaccg gcgacgcgtt
     2941 ccacatgacc tcgcccgcgc ccaacggcga gggtgcggtg cgcgcgatgc agatggcgct
     3001 cgaagtcgcc cacctggcgc cggccgcggt ggactacatc aacgcacacg ggaccagcac
     3061 cgaactcaac gacgcgagcg aaacggccgc gatcaaacac gttttcggcg agcacgcgta
     3121 caaactcgcg gtcagttcga ccaagtcgat ggtggggcac ttgctggggg cggcggccgc
     3181 ggtcgagctc atcttctgcc tgctcgccat tcgcgacgac gtcatcccgc ccaccatcaa
     3241 ccaggagcac cccgaccccg cgtgcgactt ggactacgtg cccaacgcgg cgcgcaagcg
     3301 ccgcatcgac gtggcgctgt cgaacgcgtt cgggttcgga ggccacaaca ccgcgatcat
     3361 cgtccgtcgt tacgcggacg ggtagtgcgc gcatgacggg cgcgttccga agactcctcg
     3421 gacgattcct ggggtcgaag cgcggctccg gcgcgcccgc cccgcacacc gtcgctcacc
     3481 tggtggacct cgagcagacc ctcgactatc gctttcggga ccgttcgctg ctcgtcgctt
     3541 cgttggtgca ccggtcgtat tacgcgggca ccgagatcaa cgccgccttg cagtcgaacg
     3601 aacgcatgga gttcctgggc gactcggtgc tgtcgctcgt ggtcaacgag cacctgtaca
     3661 agaggtaccc cgagcggacc gaaggcgagc tcaccaagat gaaatcggtg gtggtgagca
     3721 agcagatcct cgcccacctc gcgaagaaga tggatctcgg ccgttacgtg ctggtcagcg
     3781 agaacgcgca gcgcgccggt gtcagcacga tggagagtgt cgtagccgac gcgctcgagg
     3841 ccgtgttcgg cgccgtgttc atcgacggcg gattcgaagc ggcccggcgc gtggtcctcg
     3901 gcgtgttgcc cgataacttg ggcgaggtca tctcccagga agggacgatc aactacaaga
     3961 gcctcctgca agagtacatc caggcgctgc acaaggtgcc gccgcgttac cgcgtgcact
     4021 ccacgaccgg tcccgaccac gacaagcagt tcgcggtcga agtggtcgtg aaggggaaca
     4081 tcctggggtc gggcacgggc aagaccaaga aggacgcgga gcagaaggcg gcgcgcgaag
     4141 cctaccgacg cctgacgaac tcgcccgccg agggctaggg cccggcgccc gggcacgctt
     4201 cctgcaccga ccgccttcca tgggccgccc tgaaatcgaa gtccctgaag cgccatatac
     4261 ttacggcggc gagaaggttc gcctgtgccg cgattgccgg cttccgtgcc acctcgacgt
     4321 caccagcccg gactgttttc gccgcttctt cctccagggt atgttgcagt gcacgtactt
     4381 cgtgtacggc tactgcttcc tggtggatcg ccaaggctgc gtgttcgaca acggcgggga
     4441 ccacgaccag tactggctcc agctcctcaa ctcctacttc cataatgtgg ccggtcgcga
     4501 agaggacgac gacgaattcg cgcggcgtcg cgccgagctg atccacctcg tctccgagca
     4561 gatcgagagc cgccaggttt cgctgtgtcg gtacttcgag gagatctaca acaagatcct
     4621 cgaggacggc ggcggcatga gcgaagacgt catggcgcgc ctcgacgaag cgtatctctc
     4681 gctcttgagc gggacgtcgc gcaagcgcta ggaccgtgag cgaggacctg agagcggagg
     4741 gaagccttcg gcgctcgcct agacctcgcc tagaagtagt acccgcagcc gatcgagagc
     4801 gacgccgcgt cgcgctcgtc gctgacggtc ggactcgcgc gctccccgcg cgcgtacgcg
     4861 ccggcggcgt caatcacgat ggatccgcgc accatccagc ccaggcccac cgagtagcgc
     4921 ctcgtctccg actcgtggtc gattcgctcg aggcggtcgt tgtcgatgcg atcggcctcg
     4981 aggaagtcgg cggcggcgcg cgactgcgcg aagccggcgc gcacgcgcag cggccagcgc
     5041 gagacgctcc attcgacgcc ggcccgcagg tcgacgacct tgcgcatcac gctctgcagg
     5101 ctcgacgtga tcacgcgctc gtcgtcgatg gtcgccttcg accagtcggc gtagcccacc
     5161 tgtgccgtga gcaggagcga gcgcgaggcc ggcaccgcga tcgccgcctc gatcgagtac
     5221 ggcgtgcggt agtcggtggt ggtgctcgtg gtctcgcgcg tgaacgagcc cacgtcgtta
     5281 tcgacttggc gggtggtttc ggtgacgacg ttcgcctcga cttcgacgac catgggtgcg
     5341 gtcagcgaga cggcgagttg tgcgcgctcg agggcgtaaa aaacgagtcc gaagcgcgcc
     5401 ccatagccgt cgacgtcggc gtcggtgtct tcatagacga atgt
//
LOCUS       DASPGK010000047         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1651503, whole genome shotgun sequence.
ACCESSION   DASPGK010000047 DASPGK010000000
VERSION     DASPGK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651503"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1966)
                     /locus_tag="VEC56_01190"
     CDS             complement(<1..1966)
                     /locus_tag="VEC56_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXV12796.1"
                     /translation="MQDRFTDRVRKVIYYARDEAARLQHDYIGTEHLLLGIVREGEGI
                     AAKVLAKLELDFEQIQQAVENMVKSSGGTLTIGEIPFTPRAKRVLELAIEEARLLGHN
                     YVGTEHLLLGLIREGEGVAAQVLAELGVDRKRVREEVLKLLGPTTTVKTRKSKKEKSD
                     TPNLDQFGRDLTQMARENKLDPIIGREQEIERVIQVLSRRKKNNPVLIGEPGVGKTAI
                     AEGLAQRIVDNKVPEILKDRRICTLDLASVVAGTKYRGQFEERLKSIINEIRQAEDVI
                     IFIDEIHTIVGAGGAEGAIDASNMLKPALARGELQCIGATTLDEYRKYIEKDGALERR
                     FQPIMVNPPTPEETIQIIFGLRDKYEAHHRVKINDDSVRAAVFLSERYIHDRFLPDKA
                     IDVIDEAGSRARLSVVTAPSELTDLEREIEDVAREKESAIQAQEFEKAARYRDREKEL
                     RKKLEDIRKNWTETKQAQETVVTAKDISKVVAAMTGIPVSEVEKEETEKLLHLEDAIR
                     RRVIGQEEAVKAVARAVRRNRAGLRDPKRPIGSFIFLGPTGVGKTELAKSLAEVLFES
                     DEALIRIDMSEYMEKFAVSRLIGAPPGYVGYDDGGQLTEKVRRKPYSVVLLDEIEKAH
                     PDVFNILLQVMEEGQLTDSYGRRVDFRNTVL"
     gene            complement(1988..3058)
                     /locus_tag="VEC56_01195"
     CDS             complement(1988..3058)
                     /locus_tag="VEC56_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HXV12797.1"
                     /translation="MSGFDANDIVGKPGTWLDGSGDENEIVLSSRVRIARNLHGHRFT
                     HNCDNAELDAILRKSLDAALRTRAFARSRYIAMGEISTLDRQLLAERHLVSRDFLHNS
                     ASRGVLFTPDEATSLMINEEDHLRIQGFAAGFDLGGAFRRANDLDDQLSPDVEIAYHE
                     RLGFLTACPTNLGTGMRVSVLVHLPGLVHSKEIHKLLESLRKLNHSIRGFFGEGSEVM
                     GNFFQLSNSAALGTPEDVIVKNLREMVVKVIQYERRSRELLFRKAQRLLEDKVWRAYG
                     LLRYARSVNTKEALSLISAVRLGVGAGLVRDVPIQTLNELVVYIQPAHLQKRRGTTMD
                     AQERDVARAECIREALTRSGRG"
     gene            complement(3055..3567)
                     /locus_tag="VEC56_01200"
     CDS             complement(3055..3567)
                     /locus_tag="VEC56_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HXV12798.1"
                     /translation="MVTCQNCKVRPAKVHFTEIVNDNMVAMNLCLECAEEKGIDVNKA
                     GSYGLGDLVAGLIDDTVDSEAERISKVRCPSCGYEYSDFKKIGRLGCPECYDAFEAQL
                     VQVLRHVHGSTQHAGKKATQVGERAALRERMTVLREELTEAIRTEDYERAAAIRDEIR
                     GLEAQVEDDA"
     gene            complement(3575..>3782)
                     /locus_tag="VEC56_01205"
     CDS             complement(3575..>3782)
                     /locus_tag="VEC56_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12799.1"
                     /translation="LVNDAAVVFADEPTGNLDDDTGESIQTLMRDLATSERRTFVVVT
                     HKRRFGAYADRMLVLADKHLSEAE"
BASE COUNT          572 a         1247 c         1181 g          782 t
ORIGIN      
        1 tcagcacggt gttgcggaag tcgacacggc ggccgtagct gtcggtgagc tgaccctctt
       61 ccatgacctg caggaggata ttgaacacgt ccgggtgcgc cttctcgatt tcgtcgagca
      121 acaccaccga gtacggcttg cgacggacct tctcggtcag ctggccgccg tcgtcgtagc
      181 cgacgtatcc cggcggggcg ccgatcaagc gcgagaccgc gaacttctcc atgtactccg
      241 acatgtcgat gcgaatgagc gcctcgtcgg actcgaacag cacttcggcg agcgacttcg
      301 cgagctcggt cttgccgaca ccggtgggcc cgaggaagat gaagctgccg atggggcgct
      361 tgggatcgcg cagccccgcg cggttgcggc gcacggcgcg cgcgaccgcc ttgaccgctt
      421 cttcctggcc gatgacgcgg cggcgaatag cgtcttcgag gtgcaagagc ttctcggtct
      481 cttccttctc gacctcgctg accggaatgc cggtcatcgc cgcgaccacc ttcgagatgt
      541 ccttggccgt aaccacggtc tcctgcgcct gcttggtctc ggtccagttc ttccgaatgt
      601 cctccagctt cttgcgcaat tccttctcgc ggtcgcggta cctcgcggcc ttctcgaatt
      661 cctgggcctg aatggccgat tccttctcgc gcgcgacgtc ttcgatctcg cgctcgaggt
      721 cggtgagctc gctgggtgcc gtcaccaccg agaggcgcgc gcgcgatccc gcttcgtcga
      781 tcacgtcgat ggccttgtcg ggcaagaagc ggtcatgaat gtagcgctcc gagaggaata
      841 cggccgcgcg caccgagtcg tcgttgatct tgacgcggtg gtgggcctcg tacttgtcgc
      901 gcagcccgaa gatgatctgg atggtctcct cgggcgtggg cgggttgacc atgatgggct
      961 ggaaacggcg ctcgagcgcc ccgtccttct cgatgtactt gcggtactcg tcgagggtgg
     1021 tggcaccgat gcactgcagc tcgccgcgcg cgagcgcggg cttgagcatg ttggacgcgt
     1081 cgatggcgcc ctcggcgcca cccgcaccca cgatggtgtg gatctcgtcg atgaagatga
     1141 tgacgtcttc ggcctggcgg atctcgttga tgatcgactt caggcgctcc tcgaactgac
     1201 cgcgatactt ggtgcccgcc accaccgacg ccaggtcgag tgtgcagata cggcggtctt
     1261 tcaggatctc cggcaccttg ttgtcgacga tgcgctgcgc gaggccttcc gcgatcgcgg
     1321 tcttgcccac accgggctcg ccgatcaaga ccgggttgtt cttcttgcgg cgactcaaga
     1381 cttggatgac gcgctcgatc tcctgctcgc ggccgatgat ggggtcgagc ttgttctcgc
     1441 gcgccatctg ggtgaggtcg cgcccgaatt ggtccaggtt gggggtgtcg ctcttttcct
     1501 tcttggactt ccgggtcttg acggtggtgg tgggaccgag gagcttcagt acttcttcgc
     1561 gcacgcgctt gcggtcgacg ccgagctcgg ccagcacctg cgccgcgacc ccttcgccct
     1621 cgcggatcaa cccgagcagg aggtgctcgg tgccgacgta gttgtgaccc aaaagccgcg
     1681 cttcctcgat ggcgagttcg agcacgcgct tggcgcgcgg cgtgaaggga atctctccga
     1741 tggtgagcgt gccgccggag ctcttgacca tgttctcgac ggcctgctgg atctgctcga
     1801 agtcgagctc gagcttcgcg agcaccttgg ctgcgatgcc ttcgccttcg cgcacgatcc
     1861 cgagcaagag gtgctcggtg ccgatgtagt cgtgctggag acgcgcggct tcgtcgcgcg
     1921 cgtagtagat gacctttcga actcgatccg taaagcggtc ctgcatccgt gctccgtctt
     1981 cgttggtcta accccgtccg ctccgcgtca gcgcctcgcg gatacactcc gcgcgcgcca
     2041 cgtcgcgctc ctgcgcgtcc atcgtcgttc cccggcgctt ctggaggtgc gccggctgga
     2101 tgtagacgac cagctcgttg agcgtctgga tcggcacgtc acgtaccaac ccggccccca
     2161 ctcccaaccg caccgccgaa atgaggctca acgcttcctt ggtgtttaca ctacgagcat
     2221 aacgaaggag gccataggcc cgccagacct tatcctcgag caatctctgt gccttgcgaa
     2281 aaagtagctc ccgcgagcgc cgttcgtact ggatgacctt gaccaccatc tcgcgcaagt
     2341 tcttgacgat gacgtcttcg ggcgttccca gggcggcgct gttcgagagt tggaagaagt
     2401 tccccatcac ttccgatcct tctccgaaga acccccgaat cgaatggttg agcttgcgca
     2461 gcgactccag gagcttgtgg atctccttgc tgtggaccag tcccggcagg tgtacgagca
     2521 cggatacccg catgccggta cccaagttgg tggggcacgc ggtcagaaag cccaggcgct
     2581 cgtggtacgc gatctcgacg tccggggaga gctggtcatc caggtcgttg gcgcgccgaa
     2641 acgcgccccc gaggtcgaat ccggccgcga aaccctggat gcgcaggtgg tcctcttcgt
     2701 tgatcatcag gctggtcgcc tcgtcggggg tgaagagcac cccgcgggag gccgaattgt
     2761 gcagaaaatc gcggctgacc aggtgccgct cggccagcag ctggcggtcc agcgtcgaga
     2821 tttcccccat cgcgatgtag cggctacgcg caaacgcccg cgtccgcagc gccgcgtcca
     2881 gtgacttcct caagatggcg tcgagttccg cgttgtcgca attatgggtg aaacgatgcc
     2941 catgcaggtt ccgcgcgatg cgcacgcggc tggacagcac gatctcgttt tcgtcgccgc
     3001 tgccgtcgag ccaggtgccc ggctttccca cgatgtcgtt ggcgtcgaat ccgctcatgc
     3061 gtcgtcctcc acttgcgcct cgagcccgcg aatctcgtcg cggatggcgg ccgcgcgctc
     3121 gtagtcctcg gtccgaatcg cttccgtgag ctcttcgcgc agcaccgtca tgcgctcgcg
     3181 caaggcggcc cgctcgccca cctgggtcgc cttcttgccc gcgtgctggg tgctcccgtg
     3241 cacgtgacgc aagacttgca cgagttgcgc ttcgaaggcg tcgtagcact cggggcatcc
     3301 cagacgcccg atcttcttga agtcggagta ctcgtagccg cacgacgggc agcgcacctt
     3361 cgagatgcgc tcggcctcgg agtcgacggt gtcgtcgatc agtcccgcca ccaaatcgcc
     3421 cagcccgtag ctgcccgcct tgttgacgtc gatacccttc tcctcggcgc actccaaaca
     3481 caggttcatg gccaccatat tgtcgttcac gatctccgtg aagtggacct tggccggacg
     3541 cactttgcag ttctgacacg tcaccatggc tgcgctactc cgcctcgctc aggtgcttgt
     3601 cggccagtac cagcatccgg tccgcgtacg cgccgaagcg gcgcttgtgc gtgaccacca
     3661 cgaaggtccg ccgttcgctc gtggccaagt cgcgcatgag cgtttggatc gattcgcccg
     3721 tgtcgtcgtc gaggtttccg gtgggctcgt ccgcgaatac caccgccgcg tcgttgacca
     3781 gg
//
LOCUS       DASPGK010000048         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_363281, whole genome shotgun sequence.
ACCESSION   DASPGK010000048 DASPGK010000000
VERSION     DASPGK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363281"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1221)
                     /locus_tag="VEC56_01210"
     CDS             complement(121..1221)
                     /locus_tag="VEC56_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXV12800.1"
                     /translation="MTRVGRHDADFAALAADAAWEALAGADASAIEAVFLASFAPREL
                     CGLSDPAGTIASVLAARVPGVRAPLHGPFKTGGEAMFHALEFMRAARGDVLVMGCEKM
                     MHLDAGTAAGLLAPRVSDPVEARHGATLPSLAALATLAYARAQRVPARAFDEVAVKNH
                     AHAALNPLAHFRRAVTVDEVRASPLVADPLRRHHCAPMSDGAAACVLSFGAGAVRVTG
                     WGAGLDAPRFSERARPETFRAAHAAATAAFAMSGRSAREVDVVEIHDAFAPFELMNLE
                     AMGFFEAGDAWRALARGQLSPGGRVAVNPSGGMKARGHPIGVCGLTSMHEVVAQLTGA
                     ADARQHPGARVALIQSAGGVAPDCYAFVVEAT"
     gene            1338..1763
                     /locus_tag="VEC56_01215"
     CDS             1338..1763
                     /locus_tag="VEC56_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HXV12801.1"
                     /translation="MIHRVAVAALLAGLATQALKVVIEWARTRRLNLLRFFDNGGMPS
                     SHTALVTTLTIGVARYAGVSSSLFAVTLLFSLYFMFEAAGLRQEVGNQARVLNDLVEE
                     LRHTHHVDPKRLKELVGHTWGEVAGGFVFGLIVSAVAFR"
     gene            1875..3656
                     /locus_tag="VEC56_01220"
     CDS             1875..3656
                     /locus_tag="VEC56_01220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HXV12802.1"
                     /translation="MKPVNRISLALVLGAATMQGSCGTDDPTRVGRPPVVESFTPSSK
                     SLTVYVGDVVDFRLRAFDPDLDPLTTSFAVDGVTVSGGDRFEYHVDDVGDVVVRGTVG
                     DGEHVSFIEWRLKREIPVNLTPSFTAALPIEENPTLVIGNDMDFGVQAVDPEGVPLTY
                     KFTVDDVLAAEERQFTYRGLSTGFKIVKAVVSDGVNSIARQWRLKVTEIPDAIPPGEI
                     DIVFAGTGTNPGEIDLEWIAVGRDGMTGVASQYRVRTLPTPILTEQDWSRASERPGVP
                     APVTAGETMSMTLTGLQPARTTYVAVRAEDDFGNQSPLLETESAVTRGMYVSGVVLDA
                     RTGLPVPGALVSFGLSTVTADGTGAWELSELGAATGDIVVRDEPGEGLGAYYDYALPY
                     TVVHDDRVELFLLPDVPLETSTFPDFLTWFRSMTDAAGNPYGAETRRWQLPITLYVRA
                     FNYGGLDYRAAIERAAGEFNDILGVTVFNVVTTGLTNGVETVYVDGLPQDNYGVKEWT
                     SDWYPRRGLIEFRTVYSAPTTSVLEVVARHELGHVLGMNHSTDWAHLMVGGLAPQVAH
                     FSNDEIAIIQCRYHLPRGWDNRRFERD"
     gene            3826..>6089
                     /locus_tag="VEC56_01225"
     CDS             3826..>6089
                     /locus_tag="VEC56_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV12803.1"
                     /translation="MTLDKFTEKAQSALSDANEAAFLKGHAEVTAWHLLGSLCDQEDG
                     LVPALFQKMQIDPSRVRAVVEQRLDAMPTQEGGQALPSTDFRAALQGAVTEAKGLKDQ
                     FVSTEHLLLALLRDRKSPVGEYFQSINVTRDRVLAAIKELRGGESVVDANPEAKLQAL
                     EKFTVDLTARARAGKLDPVIGRDEEIRRVSQVLSRRTKNNPVLIGDPGVGKTAVVEGL
                     AQRIADGDVPDILRDKRVLALDMGSLVAGTKFRGEFEERVKALMKAIQHSEGEVILFI
                     DELHTLVGAGAAEGSLDASNMIKPALARGELHCIGATTMSEYRKHVEKDAALERRFQV
                     VIVHEPNVEETIAILRGLKERYEVHHGVKITDGALVAAARLSDRYITSRFLPDKAIDL
                     VDEAASRLKLELDSMPAELMSMKEKITQLTIEKKVLEKESDADSKKRKQEIERRLADI
                     EELYTAQRARWENEKTVIDRIRTVKERIEAARREEAAAIRENRLESVGEIRYGTLPKL
                     ARELEQTNAELEKLQQDGGLLREVVTAEEVAEIVARWTGIPVSKMMESDREKLLTLEA
                     RLSERVVGQREAIEAVSEAVRRVRAGLGSPHKPMGSFVFVGPTGVGKTELAKALAETL
                     FDDETRIVRVDMSEYMEKHSVARLIGAPPGYVGYEEGGYLTETVRRQPYSVILLDEIE
                     KAHPDVFNILLQILDDGRLTDGQGRTVDFANTMIIMTSNLGTSQERFRDQAEMRESVM
                     RAMRAAFKPEFLNR"
BASE COUNT         1080 a         2040 c         2041 g          928 t
ORIGIN      
        1 agacgcgcgc cctaccgtca cgcgtcaccg cgacgcccaa tcgaaacggc gccccgaagg
       61 ggttgatcct caccgtggtg accagcacca gagtggcccg cacgcgcgat cgccgtcgcg
      121 tcatgtcgct tccaccacga aggcgtagca atcgggtgcc acaccgccgg cactctggat
      181 caacgccacg cgcgcacccg ggtgctggcg agcgtccgcg gcgcccgtca actgggcgac
      241 gacttcgtgc atcgaggtca ggccgcacac gccgatgggg tgcccgcgcg ccttcatgcc
      301 gccggacgga ttgaccgcca cgcgcccgcc cggggaaagc tgaccgcgcg cgagcgcgcg
      361 ccacgcgtcc ccggcttcga agaatcccat cgcttcgagg ttcatgagtt cgaagggcgc
      421 gaaggcgtcg tggatctcga ccacgtcgac ctcgcgcgca ctccgacccg acatcgcgaa
      481 ggcggcggtg gcggcggcgt gcgccgcgcg gaaggtctcc ggacgcgcgc gctcggagaa
      541 gcggggggca tccaggcccg cgccccagcc ggtgacgcgc acggcacccg caccgaagga
      601 aagcacgcac gcggcggctc catccgacat gggcgcgcag tggtggcggc gaagcgggtc
      661 ggcgacgagc ggactcgcac gcacctcgtc gaccgtgacc gcgcgccgga agtgggccag
      721 cggattgagc gcggcgtggg catggttctt caccgccacc tcgtcgaagg cccgggccgg
      781 cacgcgctgc gcgcgcgcgt acgcgagggt ggcgagcgcc gcgagcgagg gcagagtggc
      841 gccgtgccgc gcttcgaccg ggtccgagac gcgcggtgcc aggagaccgg cggccgtacc
      901 cgcgtcgagg tgcatcatct tctcgcaccc catcacgagg acgtccccgc gcgccgcgcg
      961 catgaactcg agcgcgtgga acatcgcctc gccgccggtc ttgaacgggc cgtgcagcgg
     1021 cgcgcgaacg ccgggcacgc gagccgcgag cacgctcgca attgtgcccg cgggatccga
     1081 gagaccgcac aactcgcgcg gcgcgaagga ggcgaggaac acggcctcga tcgccgacgc
     1141 gtcggcgccg gccagcgctt cccacgcggc gtcggcggcc agcgcggcga agtccgcgtc
     1201 gtgccggccg acgcgtgtca tggccgcggt tttgaccttg atttcccgca cggtttccag
     1261 gataaataag ccccgatttc cctgtccaac gaaaccgcgc ggacgccgag cgtcgcatga
     1321 gcccgggggt gtgtcgaatg atccatcgcg tcgccgtcgc cgcgttgctc gcggggctcg
     1381 ccacgcaagc gctcaaggtc gtgatcgagt gggcgcgcac gcgacgcctg aatctcctgc
     1441 gcttcttcga caacggcggc atgccgtcgt cgcacacggc actcgtgacc acgctcacga
     1501 tcggggtcgc gcgctacgcc ggcgtgagct cctcgctctt cgcggtcacc ttgctgttca
     1561 gcctctactt catgttcgaa gcggccggcc tgcgccaaga agtgggcaac caggcgcggg
     1621 tcttgaacga cctggtcgag gagcttcgcc acacccacca tgtcgatccc aagcgcctga
     1681 aggagttggt ggggcataca tggggggagg tcgccggggg cttcgtcttc ggcctgatcg
     1741 tgtccgcggt ggccttccgg tagcccgttt cgggccttcc cccccccgcc cgagtccagg
     1801 cttgtcgggc cgcgccgcgg ggcggtatat tcgatccatc gcgtggaccc tcgcccccaa
     1861 ggatgcgcgc gcctatgaag cccgttaatc gaatttcgct cgcgctcgtg ctgggcgcag
     1921 cgacgatgca gggttcctgt ggaaccgatg accccacccg cgtcggccga ccccccgtcg
     1981 tcgagtcgtt tactcccagc agtaagtcgc tgaccgtcta cgtcggcgac gtggtcgatt
     2041 tccgcctgcg cgcgttcgac cccgatctcg atccgttgac cacctcgttc gccgtggacg
     2101 gcgtgacggt atccgggggc gatcgcttcg agtaccacgt cgacgacgtg ggcgatgtcg
     2161 tcgtgcgcgg cacggtcggc gatggcgagc acgtctcctt catcgaatgg agactcaagc
     2221 gcgaaatccc cgtcaatctg acgccgtcat tcaccgccgc cctgccgatc gaagagaatc
     2281 ccacgctggt gatcggcaac gacatggatt tcggggtgca ggcggtcgac cccgagggag
     2341 ttcccctcac gtataaattc accgtcgacg acgtgctcgc ggccgaagag cgccagttca
     2401 cgtatcgcgg actctcgacc ggtttcaaga tcgtcaaggc ggtggtttcg gacggggtga
     2461 attcgatcgc acgtcagtgg cggctcaaag taaccgagat acccgatgcc atcccgcccg
     2521 gggagatcga tatcgtcttc gccgggaccg gtaccaaccc gggcgagatc gacctggaat
     2581 ggattgccgt cggtcgggac gggatgaccg gcgtcgcgtc acagtaccgc gtgcgaacgc
     2641 tgccgacgcc gattctgacc gagcaagact ggagccgcgc gagcgagcgc cccggggttc
     2701 ccgcgccggt gaccgcgggc gagacgatgt cgatgacgct aaccggcctc cagcctgcgc
     2761 gcaccacgta cgtggcggtg cgggccgagg acgatttcgg caatcagtcg ccgctgctcg
     2821 aaaccgagtc ggcggtgacg cgcggcatgt acgtctccgg tgtcgttctc gatgcacgca
     2881 cggggctgcc ggtaccggga gcgctggtca gcttcggact gtcgacggtg acggccgacg
     2941 gcaccggggc ctgggagctc tccgagttgg gggcggccac cggcgacatc gtggtccgtg
     3001 acgagccggg tgaaggcctc ggcgcgtact acgactacgc gcttccgtac accgtcgttc
     3061 acgacgatcg ggtggaactg ttcctgctac cggacgtccc gctcgagacg agcacgtttc
     3121 ccgactttct cacctggttc cgcagcatga ccgacgccgc cggcaatccc tacggcgccg
     3181 agacgcgacg ctggcagctg cccatcacgc tctacgttcg cgcgttcaac tatggaggac
     3241 tggactaccg cgccgccatc gagcgcgccg cgggcgagtt caacgacatc ctgggcgtga
     3301 ccgtgttcaa cgtcgtcacc accggtctga cgaacggcgt cgagaccgtc tacgtcgacg
     3361 gcttgcccca ggacaactac ggcgtcaagg agtggaccag cgattggtat ccgcgacggg
     3421 ggctgatcga gtttcgaacc gtgtactcgg ccccgaccac gtcggttctc gaggtggtcg
     3481 cccgccacga gctcggtcat gtgctcggca tgaaccacag caccgactgg gcgcacctca
     3541 tggttggcgg tttggccccg caggtggccc atttctcgaa cgacgaaatc gcgatcattc
     3601 agtgccgcta tcacctcccg cgcggatggg acaaccgccg gttcgagcgc gactaacccg
     3661 cctattcctg ccggacccgc caactatggc tgccatgttg gcatgggccg gcgtcgggct
     3721 cggcgcgcca tagcgccgga atcttgtaac tcgttttaaa ataacaagta atcaaccacg
     3781 ggcgggtggc accgatcctg cgtgggttca ccccaggagg taaccatgac gctcgacaag
     3841 ttcactgaaa aagcccaaag cgccctgagc gacgccaacg aggctgcgtt cctgaaaggc
     3901 cacgccgagg tcaccgcgtg gcacctgctg ggcagtcttt gcgatcaaga agacgggctc
     3961 gtccccgcgc tcttccagaa gatgcagatc gacccctcgc gcgtgcgcgc cgtggtcgag
     4021 cagcgcctgg atgcgatgcc gacccaggag ggcgggcagg cgctcccatc caccgacttc
     4081 cgcgcggcgc tccagggtgc ggttaccgag gcgaagggac tcaaggacca gttcgtctcg
     4141 accgagcacc tgttgctggc gttgctgcgg gaccgcaagt cgccggtcgg cgagtacttc
     4201 caatcgatca acgtcactcg tgaccgcgtg ctcgcggcga tcaaggaact ccgcggcggg
     4261 gagagcgtcg tggacgccaa ccccgaagcc aaattgcagg cgctcgagaa gttcacggtc
     4321 gacctgaccg cgcgcgcgcg cgccggcaag ctcgaccccg tcatcgggcg cgacgaagag
     4381 atccgtcgcg tcagccaggt gctctcgcgc cgcaccaaga acaacccggt tctgatcgga
     4441 gatcccgggg tgggcaagac cgccgtcgtc gagggcctcg cgcagcgcat cgcggacggc
     4501 gatgtgcccg acatcctgcg cgacaaacgc gtgctcgcac tcgacatggg atcgctggtc
     4561 gcgggcacca agttccgcgg cgagttcgaa gagcgcgtca aggctctcat gaaagcgatt
     4621 cagcacagcg aaggcgaagt gatcttgttc atcgacgaac tgcatacgct ggtgggcgcg
     4681 ggcgcggccg agggctcgct ggacgcatcc aacatgatca agcccgcgct cgcacgcggc
     4741 gagctgcact gcatcggcgc caccaccatg agcgagtacc gcaaacacgt cgagaaagac
     4801 gccgcgctgg agcggcgctt ccaggtcgtc atcgtacacg agcccaacgt cgaggagacg
     4861 atcgcgatct tgcgcggcct caaggagcgc tacgaggtgc accacggcgt caagatcacc
     4921 gatggcgcgc tggtggcggc cgcgcgcttg tcggaccggt acatcacgtc gcgcttcctt
     4981 cccgacaagg ccatcgacct cgtggacgag gccgcctcgc gcctcaagct cgaactcgac
     5041 agcatgccgg ccgagctcat gagcatgaaa gagaagatca cgcagctcac catcgagaag
     5101 aaggtcctcg agaaggagtc ggacgccgac tcgaagaagc gcaagcaaga gatcgagagg
     5161 cgcctggccg acatcgaaga actctacacc gcccagcgcg cgcgctggga gaacgagaag
     5221 acggtcatcg atcggattcg aaccgttaag gagaggatcg aggcggcgcg ccgcgaagaa
     5281 gcggcggcca tccgcgagaa ccggttggag agcgtgggcg agatccgcta cgggacgctc
     5341 cccaagctcg cgcgcgagct cgagcagacc aacgcggagc tcgagaagct ccagcaagac
     5401 ggcggcctac tgcgcgaagt ggtcacggcc gaagaagtgg cggagatcgt ggcgcgctgg
     5461 accggcatcc cggtcagcaa gatgatggag tccgaccgcg agaagctctt gacgctcgaa
     5521 gcacggcttt ccgagcgcgt ggtgggacag cgcgaagcga tcgaggcggt atcggaggcg
     5581 gtacgccgcg tgcgcgccgg gctgggcagt ccccacaaac cgatggggtc gttcgtcttc
     5641 gtgggtccca cgggcgtcgg caagaccgag ctcgcgaagg cgctcgccga gacgctattc
     5701 gacgacgaga cgcggatcgt tcgcgtggac atgtccgagt acatggagaa gcactcggtc
     5761 gcgcgcttga tcggcgcgcc gccgggatac gtcggctatg aagagggcgg ctacctcacc
     5821 gaaaccgtgc ggcgccagcc ctactcggtg atcctcctgg acgagatcga gaaggcgcac
     5881 cccgacgtgt tcaacatcct cctgcaaatc ttggacgacg ggcggctcac cgacggccag
     5941 gggcgcacgg tggacttcgc gaacaccatg atcatcatga cgtcgaacct gggtacgtcg
     6001 caggaacggt tccgcgacca ggccgaaatg cgcgagtcgg tgatgcgcgc gatgcgcgcg
     6061 gcgttcaagc ccgagttttt gaaccgcat
//
LOCUS       DASPGK010000049        11028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1182976, whole genome shotgun sequence.
ACCESSION   DASPGK010000049 DASPGK010000000
VERSION     DASPGK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182976"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..582
                     /locus_tag="VEC56_01230"
     CDS             61..582
                     /locus_tag="VEC56_01230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV12804.1"
                     /translation="MNARSAILPDAEPIRALIDAHVGDGTLLPRSLPELCENIRDFVV
                     VEDDGAVVGCGALHLYGEHLAEIRSIAVMPEARGRGAGRLLVRALLRESLRQQVSCVC
                     VFTRIPEFFTPFGFVVTHETLPDKLYKDCRRCPRRHACDEIAMIRGPLPEVVAMPALA
                     RIRRPISIAGGGL"
     gene            579..1772
                     /gene="argJ"
                     /locus_tag="VEC56_01235"
     CDS             579..1772
                     /gene="argJ"
                     /locus_tag="VEC56_01235"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXV12805.1"
                     /translation="MSVQIPKGFLFAAATARIKESGNPDLAYVEAPRGATAAAMFTTN
                     RVVAAPVTVGREHLRASRGRIRALLINAGNANCATGSDGLAAARESCEALAALLSVSP
                     QEIIPGSTGIIGVPFPTSKLLAALPALLANRRADADGAYSVARAIMTTDTRMKTASTS
                     VSIDGRDVRVLGIAKGAGMIHPNMATLLVYLFTDIDASAPELRRVLRPAVAASFNSIS
                     IDGDTSTNDTVILMASGTSGACVRTTADEAAFLRAVTEICASLAAQVVADGEGVKHTV
                     TLRVDGAATHAAADRIARTLAHSLLVKTAWAGADPNWGRLLAAVGRAGVSVDPDVVAI
                     WIGGEKVCERGAAFPFDATAVHKMMSEPSYEIRVQVGGGEGAARILTGDLTAEYVRIN
                     ADYST"
     gene            1883..2269
                     /locus_tag="VEC56_01240"
     CDS             1883..2269
                     /locus_tag="VEC56_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12806.1"
                     /translation="MSESRSKSAVLAAGIAWVLVYFGCRLILDRVALPDWVRIVVALS
                     PIVPFAFFLGYAIANVRSMDELHRRVHLEALAFAFPAGVVVLMVLGLLQLVFTFHPQR
                     FNFRDAWPILLVLYCIGLAWNWRRYL"
     gene            2266..2460
                     /locus_tag="VEC56_01245"
     CDS             2266..2460
                     /locus_tag="VEC56_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV12807.1"
                     /translation="MKSRLRVLRAERNWSQAELADRLGVSRQTINAVETERYEPSLSL
                     AFKMAKLFALRVEEIFQPDE"
     gene            complement(2496..2768)
                     /locus_tag="VEC56_01250"
     CDS             complement(2496..2768)
                     /locus_tag="VEC56_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009631806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HXV12808.1"
                     /translation="MLYVVIERFKNGDPVPVYRRFRDQGRMMPDGVEYVSSWITTDMS
                     MCWQVMECDDRKLLDEWMANWDDLTDFEVIPVITSPEARERIAPKL"
     gene            complement(2776..3786)
                     /locus_tag="VEC56_01255"
     CDS             complement(2776..3786)
                     /locus_tag="VEC56_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12809.1"
                     /translation="MVKAWHILPVVGTAILAMTCGAQPDEVTHRRPSWAAGFIDSCPA
                     SEIDTTGWKTIQSRSVSFKVPLEYVDDPGGFFCGEGVHLEHGERTIGFCASCAPYVGN
                     EQWLRCTEMVGGHTAYIVASRNTVFEGKYFAGASWINGPSRMVLGGWSPDVQGQQIVL
                     AVIRTVQFKVTLDDVPVEWSPPPVDTTRPTPTFVDACPAPRIDTSRWNRFDIGSISIS
                     VPPEYVEAIGIPIDIEPDEEDRVSRTFVFGRSRVGITVDPGHSFGLTVGMNDSGCREA
                     IGEHQAHIVSGRGPGDTYFVSASWNDSPLNVFVGGSSTSMLEQRRLLAVVRTVRFSKQ
                     AE"
     gene            complement(3780..5144)
                     /locus_tag="VEC56_01260"
     CDS             complement(3780..5144)
                     /locus_tag="VEC56_01260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV12810.1"
                     /translation="MPFTKLGLHPNLLKGLKDLGFARPTPIQAEAIPPALAGKDVLAC
                     AMTGSGKTAAFLLPIAHHLIGRPRGVTRALILTPTRELAAQILDDLNDIAVHTPISGA
                     AVFGGVGMGPQEHAFRSGVDVIVATPGRLLDHFQYGYAKMNHIEFLVLDEADRMLDMG
                     FLPDIKRILRHIPARRQTLFFSATMPPPIAELTRQMLKNPATINLQRTSAPAVGITQA
                     VYPVSQELKASLLVALLRRGDVHDALVFTRTKHRANRVADYLVKQGVRAERIHGNRSQ
                     SQRTQALAGFKSGRHQVLVATDIAARGIDVEELGHVVNFDVPKAPEDYIHRVGRTARA
                     EMTGDAFTFVSPAEEGDMSRIERAIGKRLPRITVPDFDYKARPEGRLEQSSRERRDEA
                     RSKRPQHGARAGSSSGGRRPNRPKSPSRSPSPAPRSPEAPRPSSSGSRPRRPGRSRRK
                     GRSW"
     gene            complement(5198..5524)
                     /locus_tag="VEC56_01265"
     CDS             complement(5198..5524)
                     /locus_tag="VEC56_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV12811.1"
                     /translation="MTEPPTLTEPTSLRGLLQYQEHAVVSRVLLKNKGGSVTVFAFDQ
                     GEALSEHTTPFDALLVVVEGEAEVDIDRTPHAVREGEAVLLPANRPHAVRAIRAFKMM
                     LVMIKE"
     gene            complement(5585..5845)
                     /locus_tag="VEC56_01270"
     CDS             complement(5585..5845)
                     /locus_tag="VEC56_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012944860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXV12812.1"
                     /translation="MTESTWFVYIVECADDTLYAGVATDTKARVAKHNAGEGAKYTRT
                     RRPVELVYVEPASDRSTAQRREHEIKRMSAEAKRRLIADARR"
     gene            6226..9237
                     /locus_tag="VEC56_01275"
     CDS             6226..9237
                     /locus_tag="VEC56_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12813.1"
                     /translation="MRTALTRLVWLACACALIASDWGAPRLAAAGPYTRLQVLLPGEA
                     AAPGTGTGKSGAPVGQTVGVPFSVTVRACDSAWATVTTVTNSITLTSTDGSASLPPSF
                     VLSSGVATATVTLNAAGQFTFSADDNSDPTIPLATSSSVVSLLLQGFEFSRITQKNQY
                     AGQPMSIDVEAVDPNGARVGYSGPVSLNQITSYGLGRITPETITLSNGTWSGNVTMYR
                     ADETSINRGNVNIEAYLAADPSINGTSDPFTVHPGPFRKLQLVVPGQDPAPGSITGLT
                     GSPASQSAGQTFAVDVYATDAYWNPLSSSDVARITSSDPAASTPVSGALSNGFRRFTL
                     SLGSVGTQTLTVTDQTNGSITGMTSAGIPVMANAAHHFEVDPVSSPVTAGSAVPVRIR
                     ATDSTGNTVPGFSGNAIIIANTGPGSVSPEAIVFSNGIWQGDIVFRGAGGAVSFTVSD
                     FSAPPHTGTSNSFVVNAGPVAKLQVLLPGQSPAGGTASGVTGTPADQQAGTPFNLTVR
                     ACDDFWNRVPGVTHTVALSSTDAFAAMPSEVDLVNGEVVLPIRLFRSGIQTITASDVT
                     DPSIDAHTSSGVRVNGGPYARIVIVCPGESVAPGTPEGRSGTATDQSINFSFTVSIYA
                     TDAWFNPVGGVSDVIHLSSGDPLAQLPPDQAMVDGVAQMNMRLSTGGFQQITASNVSR
                     SMPTSTTQVRAISSGFHLEAEVTPTAVQAGEQFTLTVKVTNDAGSVIQEINSLVTVEV
                     QNASTQAAGRGTLLNTQFQLLQGQRSMAETYTFAESIVLIVRDDAGNAPAATEVITVT
                     PGAPTEVVLTSNPRWVGGNKHATVSASVVDLYGNGVPDQVVTFSRLAGTGTLTAIDSL
                     TASNGVARCDLHAARRPETNRVRATSGVLSDEIDIETAFVDPGAAGGYITNYPNPFHP
                     GEAPTTIAYKLADNANVTVKIYTLTGGLVFETSFASGMPGGRTGLNEFVWDGRNGRGE
                     IVASGGYVLDVQAAGTGETLHTMRRKIAVVR"
     gene            9251..10540
                     /locus_tag="VEC56_01280"
     CDS             9251..10540
                     /locus_tag="VEC56_01280"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HXV12814.1"
                     /translation="MNLNGTVRSIVTIVAAPRTACLSLAVLAGTLLAAPAANAAEATN
                     GVPGDWLSRYASARSIGLGGAFVAAADEPLGALWNPAGLTLSLQNEVHFETARYFEDT
                     AINGLSFAIPARRLPTIGFTMLSLGSGKFEKTNELNESLGSFEESDMAFLISASKSVT
                     PRLSLGANIKVVRQQVEDFDATGVGADVGVMFGLTPRLRLGASLLNIGGPTLQARDVA
                     ESFPIEMRAGAALAFLSGRGLLSLEVDHREGPGTSFRAGSEMWFYDHFGLRVGYFGDE
                     PAGGFSYRLNQAMRFDYGMSDHELGPVHHVAVSYRFGGFFANSYANPEVFSPLGETSV
                     TKFNIKARTKAETKQWRLDIVDKHNQVVRSFGGLGVPPAHVMWDGKDENGMTLADGQY
                     RYQLFVTDEEGRILRTDERTVVITTSGPQGGVPVIVD"
     gene            10666..>11028
                     /locus_tag="VEC56_01285"
     CDS             10666..>11028
                     /locus_tag="VEC56_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12815.1"
                     /translation="MAEIGMSLEDARRLPAVERDAALASIEERLDALLDGKVGAGRKA
                     AAMFLAGEIYFASGRYNEAAKAFRAAEKEAKNDPLADDAALAAIQALEAGRSAETEKE
                     WAKWLRAYGRASSASDAML"
BASE COUNT         1849 a         3692 c         3556 g         1931 t
ORIGIN      
        1 ccgccatcac gaacgtccgc gagcgattgg actccatgct ggacgacacc gaggccaaga
       61 ttgaacgctc gtagcgcgat tctgcccgac gcggaaccga tccgtgcctt gatcgacgcg
      121 cacgtgggcg acgggacgct cttgccgcgc tcgctccccg agctgtgcga gaacatccgc
      181 gacttcgtgg tggtcgagga cgacggcgcc gtggtcggct gcggggcgct ccatctctat
      241 ggcgagcatc tggccgagat tcgctccatc gcggtgatgc cggaggcgcg cgggcgcggc
      301 gccggacgac tgctggtgcg cgcattgctg cgcgaatcgc ttcgccagca ggtgtcgtgc
      361 gtgtgcgtat tcacccgcat cccggagttc ttcacacctt tcgggttcgt ggtgacgcac
      421 gagacgcttc ccgacaagct ctacaaggac tgccggcgct gcccgcggcg tcacgcatgc
      481 gacgagatcg cgatgattcg aggccccctg cccgaggtcg tcgccatgcc cgcgctggcg
      541 cggattcgtc ggccgatttc gatcgccgga ggcggattgt gagcgttcaa attccgaagg
      601 ggttcttgtt cgcggcggcc accgcacgca tcaaggagag cggcaatccc gacctcgcct
      661 acgtcgaagc gcctcgcggc gccacggccg cggccatgtt cacgaccaat cgtgtggtgg
      721 cggcgccggt gacggtcggc cgcgaacacc tgcgggcgtc gcgcggacgc atccgcgccc
      781 tcctcattaa tgcgggcaac gccaactgcg ccaccgggtc cgacggcctc gccgccgcgc
      841 gcgagtcgtg cgaggcgctg gcggctctat tgtcggtgtc cccccaggag atcatccccg
      901 gctcgaccgg catcatcggc gttccgtttc cgacgtcgaa gctgctcgcg gcgctgcccg
      961 cgttgctcgc caatcgccgc gccgacgccg atggggccta ctccgtcgcg cgcgccatca
     1021 tgaccaccga cacccgcatg aagacggcgt cgacctcggt ttcgatcgac ggccgcgacg
     1081 tgcgcgtgct cggcatcgcg aagggcgcgg gaatgatcca tcccaacatg gcgaccctgc
     1141 tggtgtacct gttcaccgat atcgacgcgt cggcgccgga gctgcgtcgc gtgctgcgcc
     1201 ccgcggtcgc ggcgagtttc aacagcattt ccatcgacgg cgacacctcg accaacgaca
     1261 ccgtgatctt gatggcgagc ggtacatcgg gcgcgtgcgt gcgtacgacg gcggacgagg
     1321 ccgcgttcct gcgggcggtg accgaaatat gcgcgtcgct ggcggcacaa gtggtcgccg
     1381 acggcgaggg tgtgaagcac acggttacgc tgcgggtcga cggtgcggcc acccacgcgg
     1441 ccgccgaccg catcgcgcgc acgctcgcgc attcgctatt ggtgaagacc gcgtgggcgg
     1501 gggccgatcc caactggggg cgcttgctcg cggcggtggg gcgcgcgggc gtttcggtcg
     1561 acccggacgt ggtcgcgatc tggatcggcg gggagaaggt gtgcgagcgc ggggccgcgt
     1621 tccccttcga cgcgaccgca gtccataaga tgatgagcga gcccagctac gagattcggg
     1681 tgcaggttgg gggcggcgaa ggcgccgccc gtatcctcac cggcgaccta actgccgaat
     1741 acgtaaggat taacgcggac tactcgacgt agccccggcc ccgccctcaa aaaacttcct
     1801 tcagaacgag taaaacatgc ttgacaaata gcaagcatgc tttacactat gtaaagtaga
     1861 cgttccgaat ggaggtttcc ctatgtctga atcacgctcg aagtcggccg tcctggccgc
     1921 cggcatcgcc tgggtactcg tgtacttcgg ctgccgattg atcctggacc gagtggccct
     1981 gcccgactgg gtccgtatcg tggtcgccct ttcgcccatc gttccgttcg cgttctttct
     2041 cggctacgcc atcgccaacg tccgctcgat ggacgagctc caccggcgcg tgcacctgga
     2101 ggcgctggcg ttcgcgtttc ccgcgggcgt ggtcgtgctg atggtgctgg ggctactcca
     2161 gctcgtgttc acgttccacc cgcagcggtt caacttccgc gacgcctggc cgatcctctt
     2221 ggtcttgtac tgcatcggtc tggcgtggaa ctggagacgg tatctatgaa gagccgactc
     2281 cgcgtcctgc gcgccgagcg caactggtcg caggccgagc tggccgatcg cttgggcgtc
     2341 tcacgccaga cgatcaacgc ggtcgaaacc gagcggtacg agccgagctt gagcctggcg
     2401 ttcaagatgg cgaagctatt cgcgcttcgg gtcgaggaga tctttcaacc cgatgaataa
     2461 cgatggaccg tctccggggg cgcgatgggc ggtcgttata atttcggcgc gatccgctct
     2521 cgcgcctccg gcgacgtgat cacgggaatc acttcgaagt cggtcaaatc gtcccaattc
     2581 gccatccatt cgtcgaggag tttgcggtca tcgcactcca tcacttgcca gcacatcgac
     2641 atgtcggtcg tgatccaact ggagacgtac tcgaccccgt cgggcatcat gcgtccctgg
     2701 tccctgaaac gccggtatac gggaacggga tcgccgttct tgaatcgctc gatgaccacg
     2761 tagagcatac gattcctatt ctgcttgctt cgaaaaacgc acggttcgta caacggccag
     2821 gagtctacgt tgttcaagca tgctggtact actcccgccg acaaacacat tcagaggcga
     2881 atcattccac gaggcgctaa cgaagtacgt atcaccgggc ccgcgacccg acacgatgtg
     2941 agcttgatgt tcgccaattg cttcgcgaca cccgctgtcg ttcataccca cggtaaggcc
     3001 aaatgagtgt ccagggtcga ccgtgatgcc cacgcgactc ctaccgaaaa cgaacgttcg
     3061 tgacacacgg tcttcctcat ctggttcgat gtcgatgggt atcccgatcg cttcgacgta
     3121 ctcgggagga accgagattg aaatcgagcc aatatcgaaa cggttccaac ggctcgtatc
     3181 gattcgaggc gcggggcaag catcaacaaa agttggggtt ggacgcgttg tgtcgacggg
     3241 cggtggtgac cactccacag gcacatcatc cagcgtcacc ttgaattgca ctgtccgaat
     3301 gacggctagg acgatttgtt gcccttgcac gtccgggctc cagccaccca ggaccatcct
     3361 gctggggcca tttatccacg acgctcccgc gaagtatttg ccctcgaaaa ctgtgttgcg
     3421 cgacgcaacg atgtaggccg tgtgtccgcc gaccatctcc gtacatcgaa gccattgctc
     3481 gttgccgaca tatggcgcgc agctagcgca gaagccaatc gttcgctcgc catgctccaa
     3541 gtgcacgccc tcgccgcaga agaagcctcc cggatcgtcg acatactcaa gtggaacctt
     3601 gaaggaaacg gagcgcgact gaatcgtttt ccacccggtg gtatcgatct cggatgccgg
     3661 gcacgagtcg atgaatccag ccgcccagct ggggcgacga tgagtcactt cgtctggctg
     3721 ggcaccacac gtcatcgcta gaattgcagt gccgacgaca ggcagtatgt gccacgcctt
     3781 caccaactcc tccccttccg tctcgacctc cctggtcgac gcgggcgcga gccgctcgaa
     3841 gaaggccggg gtgcttccgg cgagcgcggg gccggtgaag gcgagcgcga gggggacttg
     3901 ggccgattcg gacgacgtcc gccgctcgaa ctacccgcgc gggcgccgtg ctgcggacgc
     3961 ttcgagcgcg cttcgtcgcg gcgctcgcgg ctcgattgct cgaggcgccc ttccggcctc
     4021 gctttgtagt cgaagtccgg caccgtgatg cgcgggaggc gtttgccgat cgcgcgctcg
     4081 atgcgcgaca tgtcgccttc ctcggccggc gagacgaacg tgaacgcgtc gccggtcatc
     4141 tcggcgcgcg cggtgcggcc cacacggtgg atgtaatcct cgggggcctt ggggacgtcg
     4201 aagttgacca cgtgaccgag ttcttcgacg tcgatcccgc gcgcggcgat gtcggtcgcc
     4261 accagcacct gatgccgccc gctcttgaaa cccgcgagcg cctgcgtgcg ctgactctgc
     4321 gagcggttgc cgtggatgcg ctcggcgcgc acgccctgtt ttaccagata atccgcgacg
     4381 cgattggcgc ggtgcttggt gcgcgtgaac acgagcgcgt cgtggacgtc gccgcgccgc
     4441 agcagcgcca ccaacaacga cgccttcaat tcttgcgaca cggggtacac ggcctgcgtg
     4501 atgccgacgg cgggggcact agtgcgctgg agattgatgg tcgcggggtt cttgagcatt
     4561 tgccgcgtga gttccgcgat gggcggcggc atggtcgcac tgaaaaagag cgtctgccgc
     4621 cgcgcgggaa tgtggcgcag gatgcgcttg atgtcgggca agaagcccat gtcgagcatg
     4681 cgatcggctt cgtccaggac caggaactcg atgtggttca tcttcgcgta gccgtactgg
     4741 aaatgatcga gcagccgccc gggtgtcgcg acgatgacgt cgacgccgct gcggaacgcg
     4801 tgctcctgcg gtcccatccc gacgcccccg aataccgccg cgccactgat gggcgtgtgc
     4861 accgcgatgt cgttcaagtc gtcgagaatc tgggccgcca gctcgcgcgt cggcgtcaag
     4921 atgagcgcgc gcgtgacacc gcgcggacgg ccgatcaggt gatgggcgat gggcagcagg
     4981 aacgcggcgg tcttgccgct gcccgtcatc gcgcacgcga gcacgtcctt gcccgccagc
     5041 gcgggcggga tggcttcggc ttgaatgggg gtcggtcgcg cgaatccgag gtctttgagg
     5101 cccttgagaa gattgggatg aagtccgagt ttggtaaatg gcatggcgcc atcttaccgc
     5161 tttaatagag tcattgccag agcgtttaca cccgcgttca ctctttgatc atgaccagca
     5221 tcatcttgaa cgcgcgaatc gcccgcacgg cgtgcggtcg attggccggc agtaggacgg
     5281 cctcgccctc gcgcaccgcg tgcggcgtgc gatcgatgtc gacctccgcc tcgccctcga
     5341 ccaccacgag cagggcgtcg aagggtgtgg tgtgctcgct caacgcctct ccttggtcga
     5401 aggcaaagac ggtcaccgag ccgcctttgt tcttcagtag cacgcggctc accaccgcat
     5461 gctcttggta ctgcaggagg cctcggagac tcgtgggttc ggtcaacgtg ggtggctccg
     5521 tcatgatggc ccctctttcg ccgtctcatc gcacccggcc ggtgatgcgt cgcgacgcct
     5581 catcctagcg tctcgcgtcc gcgatcagcc gtcgcttcgc ttccgcggac atccgcttga
     5641 tctcgtgctc gcgcctctgc gccgtgcttc ggtcgctcgc gggctcgacg tacaccagct
     5701 cgacgggacg ccgggtgcgc gtgtacttgg ccccctcgcc ggcgttgtgc ttggcgacac
     5761 gtgccttcgt gtcggtggca acgcccgcgt agagcgtgtc gtccgcgcac tcgacgatgt
     5821 agacgaacca agtgctttcc gtcattcggc gcttcgcctt cctcccggca cgcgcccgcg
     5881 cgctcgcttg acggtcacgc gcgtccggga cgtccgtatc ctaccacggg ccttcccacg
     5941 cggccacctt cagccacgaa atcacacgaa ccaatgcccc cttgcattac acgcgcgcgc
     6001 ccaacggcca ccgggtgcca gtcacgacgc gcaccattgc gaagcgctaa cgaacgcacg
     6061 gcacgcacgg ggacgtgtgc gcgtattccc acgcacgctg gcacgtcgtt cgcacctaca
     6121 gatatcgcaa caaggcgatt tctaaccgtc gatggacgat gatggcggac cgcgtgcgaa
     6181 agtgtatcgt cgcggcctac accattcttt cgcgaggtga tcattgtgcg aacggcactg
     6241 actcgactcg tttggctcgc gtgcgcgtgt gcactcatcg cgtccgattg gggcgcgccg
     6301 cgtctcgccg cggccggacc gtacacgcgg ctccaggtcc tgctccccgg cgaagccgcc
     6361 gcccccggca ccggcaccgg caagtcgggc gccccggtgg gtcagaccgt cggtgtgccg
     6421 ttcagcgtga cggtgcgcgc gtgcgacagc gcatgggcca cggtaacgac ggtcaccaac
     6481 tcgatcacgc tgacctcgac cgacgggagc gcgtcgctac cgccgtcctt cgtgttgtcg
     6541 agcggcgttg cgacggcgac cgtcaccctg aatgcggcgg gacaattcac gttctcggcc
     6601 gacgacaact ccgacccgac cattcccctc gcgacgtcgt cctcggtggt gtcgctgttg
     6661 ctgcaaggtt tcgagttctc gcgcatcacg cagaagaacc agtacgccgg gcagcccatg
     6721 tccatcgacg tcgaagcggt ggatccgaac ggcgcgcgcg ttgggtattc gggaccggtg
     6781 agtctgaacc agatcacgag ctacgggctc ggacgcatca cgcccgaaac tatcacgctt
     6841 tcgaacggaa cgtggtccgg caacgtcacg atgtaccgcg cggacgagac ctcgatcaac
     6901 cgcggcaacg tcaacatcga ggcgtacctc gccgccgacc cgtccatcaa cggcaccagc
     6961 gacccgttca ccgttcaccc gggtccgttt cgcaagctgc agctcgtcgt gccgggccaa
     7021 gatcccgcgc cgggcagcat caccggattg acgggcagcc ccgcgtcgca aagcgccggg
     7081 cagaccttcg cggtcgacgt atacgcgacc gacgcgtact ggaatcccct ttcgagcagc
     7141 gacgtcgcgc gcatcacctc gagcgacccg gccgcaagca cgccggtgag cggcgcgctg
     7201 tcgaacggct ttcggcgctt cactctttcg ctcggctcgg tgggcacaca gaccctcacc
     7261 gtgaccgacc aaaccaacgg ctcgatcacc ggcatgacgt cggccggcat tcccgtgatg
     7321 gccaacgcgg cgcatcactt cgaagtcgat ccggtgtcct cgccggtgac ggcgggcagc
     7381 gcggtcccgg tgcgcatccg cgcgaccgat tccaccggca acacggttcc cggcttcagc
     7441 ggcaacgcga tcatcatcgc caacaccggc ccgggcagcg tgtcgccgga agcgatcgtg
     7501 ttctcgaatg gcatctggca gggcgacata gtctttcggg gcgccggcgg cgcggtgtcg
     7561 ttcacggtct ccgatttttc ggcgcccccg cacaccggca ccagcaactc cttcgtcgtg
     7621 aacgcgggtc cggttgcgaa gctgcaggtg ctgctgccgg gtcaatcgcc cgcgggcggg
     7681 accgcgagcg gcgtcaccgg cacgccggcc gaccagcagg cgggaacgcc gttcaacttg
     7741 accgtgcgcg cgtgcgacga cttctggaat cgcgtgcccg gggtgaccca cacggtcgcg
     7801 ctgtcgtcca ccgacgcgtt cgcggccatg ccgagcgagg tggacctcgt caacggcgag
     7861 gtcgtgctgc ccattcgcct gttccgctcg ggcatccaga ccatcaccgc gtcggacgtg
     7921 accgatccgt ccatcgacgc ccatacctcg agcggcgtgc gggtcaacgg cggtccgtac
     7981 gcgcgcatcg tcatcgtctg tccgggcgaa tcggtggcgc cgggaactcc ggagggtcgc
     8041 tccggaaccg cgaccgacca gtcgatcaat ttctcgttca ccgtgtcgat ctacgccacc
     8101 gacgcttggt tcaaccccgt cggcggcgtc agcgacgtca tccacttgtc gtcgggcgac
     8161 ccgctcgcgc agctgccgcc cgaccaggcc atggtcgacg gtgtggcgca gatgaacatg
     8221 cgcctctcga cggggggctt ccagcagatc accgcctcca acgtctcgcg ctcgatgccc
     8281 acgagcacca cccaggtgcg cgcgatctcg agcggattcc atctggaggc cgaggtgacc
     8341 ccgaccgcgg ttcaggccgg cgagcaattc accctcacgg tcaaggtgac caacgacgcc
     8401 ggcagcgtca tccaggagat caactctctg gtcacagtcg aggtccagaa tgcgtccacg
     8461 caggcggcgg gccggggaac gctgctcaat acgcagttcc agctcctgca gggccagcgc
     8521 tccatggcgg agacctacac gttcgcggag tccatcgtgc tgatcgtgcg agacgacgcg
     8581 ggaaacgcgc ccgccgcgac cgaagtgatc acggtgacgc cgggcgcacc cactgaagtg
     8641 gtgctcacga gtaacccaag gtgggtgggt ggcaacaagc acgcgacggt gagcgcgtcg
     8701 gtcgtcgatc tgtacggcaa cggcgttccc gaccaggtgg tcacgttcag tcgtctggcc
     8761 gggaccggca cactgacggc catcgactcg ctcacggcca gcaacggtgt cgcgcgctgc
     8821 gacctgcacg ccgcgcgccg ccccgagacc aaccgcgtgc gcgcgacgtc gggcgtgttg
     8881 agcgatgaaa tcgacatcga gacggcgttc gtcgacccgg gtgcggccgg cgggtacatc
     8941 accaactacc cgaacccgtt tcatcccggc gaagcgccca ccaccatcgc ctacaagctc
     9001 gcggacaacg ccaacgtgac ggtcaagatc tacaccctca cgggcgggct cgtgttcgag
     9061 accagcttcg cgtccggaat gccgggcggc agaaccggcc tcaacgaatt cgtatgggac
     9121 ggccgcaacg gccgcggcga gatcgtcgcc agcggcggtt acgtgctgga cgtccaggcc
     9181 gccggaaccg gcgaaacgct gcacaccatg cgccgcaaga tcgcggtcgt gcgctgaccg
     9241 ggaggacgac atgaatctca acggaaccgt tcgttcgatc gttaccatcg tcgccgcgcc
     9301 gcggacggcg tgcctttcct tggccgtcct ggccgggact ctgctggcgg ctcctgcggc
     9361 caacgccgcc gaggcgacca acggtgtgcc cggggattgg ctttcgcggt acgcgtcggc
     9421 gcgctccatc gggctcggcg gcgcgttcgt cgccgcggcc gacgagccgc tgggcgcgct
     9481 gtggaatccg gccggtctca cgctgtccct gcagaacgaa gtgcacttcg agacggcgcg
     9541 ctacttcgag gacaccgcga tcaacggctt gagcttcgcg atacccgcgc ggcgccttcc
     9601 gaccatcggg ttcaccatgc tgtccctggg ctcgggcaag ttcgaaaaga ccaacgagct
     9661 caacgagtcg ctgggatcgt tcgaggagag cgacatggcg ttcctgatct cggcgtcgaa
     9721 gagcgtgacg ccgcggctct cgctcggcgc caacattaaa gtcgtgcgcc agcaggtcga
     9781 agacttcgac gcgaccggtg tcggcgcgga cgtcggcgtc atgttcgggc tgacgccccg
     9841 gcttcgcctc ggtgcgtcgc tgctaaacat tggcggcccg acgctgcaag cccgcgacgt
     9901 ggcggagtcg tttccgatcg aaatgcgcgc cggcgccgcg ctcgccttcc tgtccgggcg
     9961 cgggctcttg agcctcgagg tcgaccaccg cgaggggccc ggcaccagct ttcgcgccgg
    10021 cagcgagatg tggttctacg accacttcgg tttgcgcgtc ggatacttcg gtgacgagcc
    10081 ggcgggtggt ttcagctacc ggttgaacca ggccatgcgc ttcgactacg gcatgtcgga
    10141 tcacgagctg ggccccgtcc atcacgtcgc ggtttcgtat cgattcggcg ggttcttcgc
    10201 caactcgtac gccaacccgg aagtgttctc gccgctcgga gagacctccg tcaccaagtt
    10261 caacatcaag gcgcgtacca aggcggagac gaaacagtgg agactggaca tcgtcgataa
    10321 gcacaaccaa gtcgtacgct cgttcggcgg gctcggtgtt ccgcccgccc acgtcatgtg
    10381 ggacggcaag gacgagaacg ggatgaccct tgcggacggt cagtatagat accaactgtt
    10441 cgtgaccgac gaagagggcc gcatactgcg caccgacgag cggaccgtcg tgatcacgac
    10501 gtcgggcccg cagggcggcg tgccggtcat cgtcgactag cggagagaac catgctctac
    10561 acgagtcaca gcacgggcgc cgtttcgagg cgcgccttcg cggtcgccgc cctggcggtc
    10621 gcgatcacaa cgaccgcact ggcggagaca tcgcgccagg aatcgatggc ggagatcggc
    10681 atgtcgctgg aggacgcgcg acgcctgccg gcggtggagc gggacgcggc gctggcctcg
    10741 atcgaggagc gcctcgacgc cttgctcgac ggcaaagtcg gcgccgggcg caaggccgcg
    10801 gcaatgttcc tcgccggcga gatttatttc gcctccggcc gttacaacga ggcggccaaa
    10861 gccttccggg ccgcggagaa agaagcgaag aacgatcccc tggcggacga cgccgcgctc
    10921 gccgccatcc aggcgctcga agccggccgc agcgccgaga ccgaaaagga gtgggcgaag
    10981 tggttgcgcg cctatggacg tgcgtcgtcc gcgtccgacg ccatgctc
//
LOCUS       DASPGK010000050         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1183010, whole genome shotgun sequence.
ACCESSION   DASPGK010000050 DASPGK010000000
VERSION     DASPGK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183010"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VEC56_01290"
     CDS             complement(<1..202)
                     /locus_tag="VEC56_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXV12816.1"
                     /translation="MRQFGAEFFGTFWLVLGGCGSAVLAAAFPGVGIGLLGVSLAFGL
                     TVLTMAFAIGHISGCHLNPAGSI"
     gene            complement(296..1426)
                     /locus_tag="VEC56_01295"
     CDS             complement(296..1426)
                     /locus_tag="VEC56_01295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV12817.1"
                     /translation="MQPKKIVFVSSLRRYGGGERWMLDTASGLRARGHDTRLVARPGS
                     VLATRAPARGIPLTELEMRGDVDPFAVATLTALVRRFQPHVIVPNLDREIRLAAAAIR
                     AARALRPRPTKPRLIPRRGSEFPLKDKRHYRIVYTGDVDRVIVNSEATKRTMMRDAPW
                     FPESKAVVIYNGIDVGPYEALAARRDDVRAKLRALIGAPADAPVVSLVGELNERKQQR
                     AVIEAAPHVLERFPATRILFVGDGDDRADLEKSIAERGLQSTILIQGFRFDVPEIIAG
                     SDALLLPSRVEGFGYVLVEAMAAAVPCIASNVSSIPEIVEDGVTGILHPVGDIGAIAN
                     AINFVLANPEAARAMGDAGQRVAHEKFTLPRMLDQVEHVLFE"
     gene            1658..>2411
                     /locus_tag="VEC56_01300"
     CDS             1658..>2411
                     /locus_tag="VEC56_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12818.1"
                     /translation="MRRGLRPESAAGAIALARVATAAALLAGLACSQTSEPSDTTTQS
                     GCVTDFSCPFGEECVGSGCAPIGASLYPHIQTASVQHRGPLDDAEVVWRAQHADLLIG
                     GIRADVARAANPNARMFEYVVTRYHRFDQGTKTASDWALAHGYNPEDFYLHFREDVDV
                     PTWESTVIVPGFPAGRVPGWNPGGPNASASSRNQSRVVGYYNGGEPWHFANLAHAGYR
                     AFLAEHIAGLIDGTWYFNQPFASGPIDGVLMDE"
BASE COUNT          410 a          860 c          765 g          376 t
ORIGIN      
        1 ctatggaccc ggccggattc aggtgacacc ccgatatgtg cccgatcgca aacgccatgg
       61 tgagcacggt caagccgaac gccagcgaga cgccgagcag cccgatcccg acgcccggga
      121 acgccgccgc gagcacggca ctcccgcatc cacccagtac cagccagaac gtcccgaaaa
      181 actcagcgcc aaactgtcgc atctgttacc tccgtggccg atctactgtc cttcgcatcg
      241 ataagggacc gtatgacacc cccgcgcccg cgtcaacctt ccccccgcgg gcagcctact
      301 cgaagaggac gtgctcgacc tggtcgagca tgcgcggcag cgtgaacttc tcgtgcgcga
      361 cgcgctgtcc tgcgtcaccc atggcgcgcg ccgcctccgg gttggccagg acgaaattga
      421 tcgcgttggc gatggccccg atgtcgccaa ccggatgcag tatgccggtg acaccgtcct
      481 cgacgatctc gggaatgctg ctcacgttgg acgcgatgca cggcaccgcg gccgccatcg
      541 cttcgaccag aacgtatccg aaaccctcca cgcgcgaggg caacagcagt gcgtccgaac
      601 cggcgattat ctcgggcacg tcgaagcgaa agccctggat caaaatcgtg ctctggagac
      661 cgcgttctgc gatcgacttc tccaagtcgg cgcggtcgtc tccgtcgccc acgaagagaa
      721 tgcgcgtggc cggaaagcgc tcgagcacgt ggggcgcggc ttcgatgacc gcgcgctgct
      781 gcttgcgctc gttcaactcg cccaccagcg agaccaccgg cgcgtccgcc ggcgcgccga
      841 tcagcgcgcg caacttcgcg cgcacgtcgt cgcggcgcgc ggccagtgct tcgtacggcc
      901 ccacgtcgat gccattataa atcacgaccg ccttgctctc cgggaaccag ggagcgtcgc
      961 gcatcatggt gcgtttggtg gcttccgaat tgacgatgac gcggtcgacg tcgccggtgt
     1021 aaacgatgcg gtagtgccgc ttgtccttga gcgggaactc gctgccccgg cgcggaatca
     1081 accgcggctt ggtcgggcgc gggcgcagcg cccgcgccgc gcggatcgcg gccgcggcca
     1141 accggatctc gcggtcgaga ttcggcacga tgacgtgggg ctggaagcgg cgcacgagcg
     1201 ccgtcagcgt ggccaccgcg aacggatcga cgtcgccgcg catctcgagt tccgtgagcg
     1261 gaatgccgcg cgcgggcgcg cgcgtcgcga gtacgctgcc gggccgggcc accaaacgcg
     1321 tgtcgtgtcc gcgcgcacgc aaccccgagg cggtgtccag catccaacgc tctcccccgc
     1381 cgtaacggcg cagcgacgac acgaacacga tcttcttggg ttgcaccaat agctccgaga
     1441 aacgaccgtg ccgcgccttg cgaaaccgga atgccaagcg cgcgacaggg agcccgagtc
     1501 tacccccgga accctcctat cgccagcgat ttccggaccc tcccgggagc gactcgaccc
     1561 catcgaccct cgagcgccgc accgcgccat gcgacgattt cgcggcatat cggttgcacg
     1621 gtactcgatg agcccctcga caccttggag cgacatcatg cggcgtgggt tacgtccaga
     1681 atcagccgcg ggcgccatcg cgctcgcgcg cgtcgcgacg gcggcggcct tgctcgccgg
     1741 cctcgcgtgc tcgcagacgt cggagccttc ggatacgacc acgcagagcg ggtgcgtcac
     1801 cgatttttcc tgtccctttg gagaggagtg cgtcggatcc gggtgtgccc ccatcggggc
     1861 ttcactctat ccgcacattc agaccgcttc ggttcagcac cggggtccgc tggacgatgc
     1921 cgaagtcgtg tggcgtgcgc agcacgccga tctcttgatc ggcggcatcc gcgccgacgt
     1981 cgcgcgcgcg gccaacccca atgcgcgcat gttcgagtac gtcgtgacgc gctaccatcg
     2041 cttcgaccaa ggaaccaaga ccgcgtcgga ttgggcgctc gcgcacgggt acaacccgga
     2101 ggacttctac ctccacttcc gcgaggacgt cgacgtgccg acctgggagt cgacggtcat
     2161 cgtgcccgga ttcccggccg gccgcgtgcc cgggtggaac ccggggggac cgaacgcttc
     2221 cgcgtccagt cgaaaccaat cgcgcgtggt cggctactac aacggcggcg agccctggca
     2281 cttcgccaac ctcgcgcacg cgggataccg cgccttcctg gccgagcaca tcgcggggct
     2341 gatcgacggc acgtggtact tcaaccagcc cttcgccagc gggccgatcg acggcgtcct
     2401 gatggatgaa g
//
LOCUS       DASPGK010000051         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1417325, whole genome shotgun sequence.
ACCESSION   DASPGK010000051 DASPGK010000000
VERSION     DASPGK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417325"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VEC56_01305"
     CDS             complement(<1..354)
                     /locus_tag="VEC56_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12819.1"
                     /translation="MKRTMLVALAAATTIACSAGTAVTTEIDYGAFSRTMGKFYLTAE
                     IGTPGSPDYRFFYADKSGHIHMYMAKDGGLVTDWEITTLGSRATALIVTDLYGDGKQK
                     LVTSSINGRLLVYDVA"
     gene            complement(396..1325)
                     /gene="mdh"
                     /locus_tag="VEC56_01310"
     CDS             complement(396..1325)
                     /gene="mdh"
                     /locus_tag="VEC56_01310"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXV12820.1"
                     /translation="MARPKIALIGGGQIGGTLALLAAQRELGDVVLYDVVEGMPQGKA
                     LDIMESRPVDGYDVDLVGTNDYRDIRGADVVIVTAGFPRKPGMSRDDLLNKNIEIMKT
                     VATNVKEHASKAFVIVISNPLDAMVFAMKRITGFPRERVVGMAGVLDSARFRTFVAME
                     LGVSVEDVTAFVLGGHGDEMVPLPRYCTVAGIPLPDLMSQEQIDRIVKRTRFAGGEIV
                     ELLKTGSAFYSPAAAAIAMAESYLKDKKRVLACAAYLEGEYGVDGLYVGVPVVIGKGG
                     VERVIEIKLAAEERKLFDVSVDHVRTLLKSLPL"
     gene            1557..1958
                     /locus_tag="VEC56_01315"
     CDS             1557..1958
                     /locus_tag="VEC56_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HXV12821.1"
                     /translation="MIRNLRSLSRLPHIVSAVAFGAAVFLMIPSCGDDEDPVDPGNGS
                     PLTVRVLATSFSPQNLTIQANQTVTWTFNGGPHTVTEGTAVGNPPSPLFDSGQRSSGS
                     FAFTFDTPGTYNYICEIHVGMGMTGTITVEP"
BASE COUNT          362 a          688 c          610 g          380 t
ORIGIN      
        1 cgcgacgtcg taaaccaaga ggcgtccgtt gatactgctc gtgaccagct tctgtttgcc
       61 gtcgccgtac aagtcggtga cgatgagcgc ggtcgcgcgc gagcccaggg tcgtgatttc
      121 ccagtccgtc acgagcccgc catccttggc catgtacata tggatatgcc ccgacttgtc
      181 ggcgtagaag aagcggtagt cgggcgaccc cggggtaccg atctcggccg tcagatagaa
      241 cttccccatc gtccgcgaga acgccccgta gtcgatctcg gtggtcaccg cggtacccgc
      301 gctgcacgcg atcgtggtgg cggcggcgag ggcgactagc atcgtgcgtt tcatgctcgg
      361 ctccttgaca ggggacgttc gtgagagcaa gcccgctaga gcgggagcga cttcaatagc
      421 gttcgtacgt ggtcgacgga cacgtcgaaa agcttgcgct cctcggcggc cagcttgatc
      481 tcgatcacgc gctccacccc gcccttcccg atcacgaccg gcacgccgac gtagagtcca
      541 tccacgccgt attcgccttc cagataggcg gcgcacgcca gcacgcgctt cttgtctttc
      601 agatacgact ccgccatcgc gatggccgcg gcggccggcg aatagaaggc gcttccggtc
      661 ttcaagagct ccacgatctc cccgcccgcg aagcgcgtgc gcttgacgat gcggtcgatc
      721 tgctcttgcg acatgaggtc cgggagcgga atgccggcca cggtgcagta gcgcggcagg
      781 ggcaccatct cgtcgccgtg gccgcccagt acgaacgccg tcacgtcctc gacgctcaca
      841 cccagctcca tcgcgacgaa ggtgcggaag cgcgccgagt ccagaacacc cgccatcccc
      901 accacgcgtt cgcgcggaaa cccggtgatg cgcttcatcg cgaacaccat cgcgtcgagc
      961 ggattggaga tgacgatgac gaacgccttc gacgcgtgtt ccttcacgtt ggtcgcgacc
     1021 gtcttcatga tctcgatgtt cttattgaga aggtcgtcgc ggctcatgcc gggcttgcgc
     1081 gggaatcccg ccgtgacgat gacgacgtcc gcgccccgga tgtcccggta gtcgttggtg
     1141 cccacgagat cgacgtcata gccgtcgacc gggcgcgact ccatgatgtc gagggccttg
     1201 ccctgcggca ttccctcgac gacgtcgtag agcaccacgt cgccgagctc gcgctgggcc
     1261 gccaaaagtg caagcgttcc accgatctgt ccgccgccga tcaaggcgat cttcggacgc
     1321 gccataaagc tcactgcctc cctggaaagg ccgcttcgcg gcctgcggcc cggtgagtta
     1381 aagaacggcg cccctggggt tcaggggcgc ccacgacgcg ggagtatacc gcctcgccca
     1441 atcgatcgta cagcccctgt tttttccggc agttgcggca ggtccatggt tcgcgatata
     1501 tttccaagca ctcgccccgg aaaccacctg tataccgatt cgatcgaggt acaaccatga
     1561 tccgaaacct tcggtcactc tcccgcttac cccacatcgt ctccgcggtc gcgttcggtg
     1621 ccgcggtgtt cctcatgatt ccctcctgcg gtgacgatga agatccggtg gaccccggca
     1681 acggctcccc gttgaccgtt cgggtactcg ccaccagctt ctccccgcaa aacctgacca
     1741 tccaggccaa tcaaacggtg acgtggacgt tcaacggcgg gccgcacacg gtcaccgagg
     1801 ggacggccgt gggaaaccct ccgtcgccgt tgttcgattc cgggcagcga tcgtcgggtt
     1861 cgttcgcgtt cacgttcgac acccccggga cctacaacta catctgcgag atccacgtgg
     1921 gcatgggaat gacgggtacc attacggtcg agccctaaga ccgcgtccac caagcagttg
     1981 acggcagcgc gcggcgtttg gtagaatcgg tgtagtctgc tatcggagcg cgccaccaga
//
LOCUS       DASPGK010000052         9064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_12684, whole genome shotgun sequence.
ACCESSION   DASPGK010000052 DASPGK010000000
VERSION     DASPGK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12684"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VEC56_01320"
     CDS             <1..453
                     /locus_tag="VEC56_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV12822.1"
                     /translation="PISILQLINLAQSTGELVFENGENAARVYFENGSVSYAEIANRR
                     MKLGEHLLSQGLITRERLERALAEKDKATRLGTALVEMGAIDARTLRRAIEEQIKEVV
                     YEIVRWRAGRFRFTSGRRPKAQDVFIDIPLDHLILEGLKRLDEEGAVR"
     gene            450..1586
                     /locus_tag="VEC56_01325"
     CDS             450..1586
                     /locus_tag="VEC56_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12823.1"
                     /translation="MTARRLVLILLAPLALFIDQPCDATTAASLSDRLRVDGDLSDWA
                     GDEWVLDATTALPERDDDSRWGPSEEIVRVGVTWDATYLYLAVEFRASAASLFAALAY
                     GPGGLASLDGAGTFRRAIDFPFALNIAALVPIRDEIALARVDDRGVLVLLDRALAPAV
                     VRAPPGQPAVFEAALPWSQLSLARPLELAVALTGEAGTGAGDAAPDPSSALPVSPGPW
                     SKTRAPLDRWLSIPADADTDGGADLGVSPRAAVTVRTDESPTRSRADGLEVETRVVPG
                     VFAPDRGQEATLEVAIEGDTVDEVFATARVYSVGGALVRVLYEDAPRAVAGGALVPSP
                     QDRWDGRDAVGRIVPGGVYVVAFEWGLVRGERAGRKSAGVAVAR"
     gene            1592..2497
                     /locus_tag="VEC56_01330"
     CDS             1592..2497
                     /locus_tag="VEC56_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12824.1"
                     /translation="MRARPLRWSPQRAAAALALAAALVVTTSARAHFEGAILSSRTAS
                     LGGAFVAIADDPSAVVDNPAGLSGLVQPSLLATYQKPFGVDGLDEGFVAVSVPARVLT
                     VGMAWFHRGLDGALSEDRFTLSLARDLKRTSEDASLSIGASVDFARVSAAGSIDENDT
                     AVSFGAGVLLRPFAFIGMGYNIRSINQPSIDLVEGGASTPLARAQAAGLSYYWDNRLT
                     VTAETFEDASSWRTRAGVELRLGPHVMLRCGLESSRASAGVGILWRSVGFDAGFLSHE
                     ALGESYVMTFRYARKEPATPYGSTR"
     gene            2481..3917
                     /locus_tag="VEC56_01335"
     CDS             2481..3917
                     /locus_tag="VEC56_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12825.1"
                     /translation="MDRRASTATYLCALAAVCLAGDASAFWPVTSRARLELRLTADEA
                     SAPWSWDAQSRSPLDDSRFMVDVTAGDARVGTLYAKGAASWNDDADAGGRVTFALEQG
                     DYLFRWNRAEVRAFGDERRFFTYDLGAALMDDDVVDDYQHRIGVRADGGTQRIGGTLL
                     VSSLDQGSDTQLVSYAKLRTGWRPLAAALSYRVQDDDATDLAIAKGEVAGFWKRATVV
                     ASYEQSGVGSGVFVPDGDWDSFGSGYAAAAPENSATFVEARLARSRIGSSAVLSGVYR
                     YALAGAAYANGLASLPAGTELHRLGLYLSHRRYALDGRLVLFDADHAYDERGVAASAR
                     AFLKDNSEMNLRAKIVDATRDDGEERTTGSAHAIYRRSLQRFMGGIDALVDDIGADAV
                     TAVGVETRINWSATSALYLRWIASGAVERSDAVYARLEFRPTARTWVTFAYGRAVAGD
                     GPYLLEDDDALPTAGTEDVFTIVVRGDF"
     gene            3919..5919
                     /locus_tag="VEC56_01340"
     CDS             3919..5919
                     /locus_tag="VEC56_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HXV12826.1"
                     /translation="MRARRVIVAAVAVWLVLTATQRARAAVRVEADEVIFTLTARDAK
                     EAFLVGDFNQWNPTVEPMQREGDQFVVRLFLVAGTYRYKFVVDGKAIADPDNRGPSPD
                     QGSPIVLVERSGGLILSTEIPEEAARARSASYQARYLGFLRDDDGDTDVEQRVDLGLR
                     ADLDRLRARAIVTTADSSWTWSPPSIEVEFDRGFVEVEMGKLSVRGFENDSTWASSGP
                     LGLVGQAGLYRYDAGFAYHGATAVAAAKHAALRARWSDETTRGDLSRASVPPLDLSSF
                     ANGTAAATTAYAYTPTFNGSDNVAVEASADAGDVASGYAFRNDTGVNPGLWVDVARAG
                     VDFATQTYATKEDRHVSAAWLSWSGLDRTTLSFAYGWGDAEARAYATASGTSDLSAPL
                     DAAAATTPVDVTRGILTSNRFVVEATSARSVATSLRWDYASFDFGGVEGTSQADVHRA
                     RADVAGTLRGWSLSGRVAYTHQRYRDTPDALYIDWPERNLWLSRWDALDAPSMVAIDL
                     EQHTVWSLKATREGTRVDAGGEALLQTLEVVDAPVHAEVRAYGDVTVRGPWYLYGDAR
                     VSWYDRAAWGVDEAFWDFYVEGGYRKGVVKLSAGFGLDPWAFDPVISDFADLGRSEFL
                     RDAIAGGVRRSDATAIGRALVERERALSDLRLFKLECVIELR"
     gene            5898..6320
                     /locus_tag="VEC56_01345"
     CDS             5898..6320
                     /locus_tag="VEC56_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12827.1"
                     /translation="MRHRAAISLSALVVLSTGCASLAPRARATGPAVADDGVRFRFFA
                     PTAHRVQLAGSWPENNWAKGDGSVGEANVGLMSDDDADGMWEIVVPLSAGRYQYLFWI
                     DEATWSLDPGNPEEVQAGPQGRASEIVVVVRDDEREIR"
     gene            6317..6688
                     /locus_tag="VEC56_01350"
     CDS             6317..6688
                     /locus_tag="VEC56_01350"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HXV12828.1"
                     /translation="MKPILWVGVALTLALAACGGPPPPAATGGPEPVAGGAVFRYRNP
                     DAKKVYLVGDFNDWSPTSDPMTDDNADGEWTLFYPLVPGTYAYKFLVDGRNWVADPTN
                     PLSEPDGFDGRNSILKLLPPG"
     gene            6957..>9064
                     /locus_tag="VEC56_01355"
     CDS             6957..>9064
                     /locus_tag="VEC56_01355"
                     /inference="COORDINATES: protein motif:HMM:NF020698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HXV12829.1"
                     /translation="MKTHRSIRITALAGVLAATVVALTAAGPGSGTANISPSSNVAAG
                     SAGEWSITYTAAEVHDNGTLRLTIPAGWTAPQDASAMSPGFVAVVTDEPTGAPGLSIA
                     GQVITVVVDTLNVGNTITIVYGSDDISMMGRASAATMVGSYAFLVESDPLGASPAPLV
                     TSPSINVIATTPASFDILPDDTTATAGDFVELHIRVLDTFGNRAPVSSNRTVNLFATH
                     GQYFDPSNHSTPITSTVIANGTNVKRVDYRPTLVPGSPHTLNVFTASGSPSLGGSEFV
                     RVTPAPMSPVQSLITATTPVVANGSAQSTVVVTSRDQFGNRRPGDTVTIDATGSAVDV
                     DPPLPTDAIGQTSGVVTNLVAEPVTVSAQINAQTITATAPISFTAGPPSGATSLVDAT
                     TNVVANGVATSVITVTVRDANNNPVSGQTVTLAVNPVPNATLSQPGGVTNALGQVTGT
                     LASTTAVQRTVTAVIAPSTPITDNAIIQFVPGPLASFQIGVDGNAIAGTVDPVTLTAR
                     DAQGNTVTSYTGTVNLRTNSGVANSVEWALGDGNGIITNVPGSDDGTYTFAAADNGVA
                     IIRVIDERVETIQITADLGGPPLGTSGNLVVTNNVADKIELVAGNGQSATVNTPVSVP
                     PLVRVVDAFNNPVPTATVTFTAVGGGGSVDVTSGGAPDSTGITGSDGRIDCDVWRLGT
                     GAGLNRLRARIASGTITSVD"
BASE COUNT         1351 a         3227 c         3094 g         1392 t
ORIGIN      
        1 cccatatcga ttctccagtt gatcaacctt gcccagtcga cgggcgagtt ggtcttcgag
       61 aacggcgaga acgccgcgcg tgtgtatttc gagaacggca gcgtctcgta cgccgagatc
      121 gcgaatcgcc gcatgaagct cggagagcac ctgctctcgc aaggcctgat cacgcgcgag
      181 cgcctcgagc gcgcactggc cgagaaggac aaggcgaccc gattgggcac cgcactggtc
      241 gagatgggcg ccatcgacgc gcgcacgttg cgccgcgcca tcgaagagca gatcaaagaa
      301 gtggtctacg agatcgtgcg ctggagagcg ggccgcttcc gcttcaccag cggccgtcgt
      361 cccaaggcgc aagacgtctt catcgacatc ccgctcgacc atctgattct cgaaggcctc
      421 aagcgcttgg acgaagaagg agcggtgcgg tgaccgcgcg ccggctcgtc ctaatcctcc
      481 tcgcccccct cgccctcttc atcgatcaac cctgcgacgc caccaccgcg gcctcgctct
      541 cggatcgctt gcgcgtcgac ggtgacctct ccgactgggc cggcgacgaa tgggtgctcg
      601 acgcgaccac cgcgcttccc gagcgcgacg acgattcgcg ctggggacct tccgaagaga
      661 tcgtgcgcgt gggcgtgacc tgggatgcga cctatctgta cctcgccgtg gaattccgcg
      721 cgtcggcggc gtcgttgttc gccgccctcg cgtacgggcc gggcgggctc gcctcgctcg
      781 acggcgccgg cacgttccgg cgcgcgatcg actttccctt cgctctcaac atcgcggccc
      841 tggtccccat ccgcgacgag atcgcgctcg cgcgcgtcga cgaccgcggc gtcctcgtcc
      901 tcttggaccg cgcgctcgca cccgcggtgg tgcgcgcgcc gccgggccag ccggccgtgt
      961 tcgaagcggc cctgccgtgg tcacagctct cgctcgcgcg accgctggag ctcgcggtcg
     1021 cgctcaccgg cgaggccggc accggcgcgg gcgacgccgc gcccgatccg tcgtcggcgc
     1081 tccccgtgag tccgggaccg tggtcgaaga cgcgcgcgcc gctcgatcgc tggctgtcga
     1141 ttccggccga cgccgacacc gacggcggcg ccgacctggg cgtgtcgccg cgcgcggccg
     1201 tgactgtgcg caccgatgag agcccgacac ggtcgcgcgc ggacgggctc gaggtcgaga
     1261 cccgtgtggt cccgggcgtg ttcgcgcccg accgcggcca agaagcgacg ctcgaggtcg
     1321 cgatcgaggg cgatacggtc gacgaggtct tcgccaccgc gcgcgtctac tcggtgggcg
     1381 gggcgctggt gcgggtcctc tacgaggacg cgccgcgcgc ggtcgcgggc ggcgcgctgg
     1441 ttccgagccc gcaggatcgc tgggacgggc gcgatgccgt cggccgcatc gtgcccggcg
     1501 gcgtatacgt cgtcgccttc gaatggggac tcgtgcgcgg cgagcgcgcg ggccggaaga
     1561 gcgccggagt cgcggtcgcg cggtgatacc catgcgcgca cgacctctcc gatggtcgcc
     1621 gcagcgcgcg gcagcggcgc tcgcgctggc ggctgcgctc gtcgtgacga cgagcgcacg
     1681 cgcccacttc gagggcgcga ttctctcctc gcggacggcg tccttgggcg gcgccttcgt
     1741 cgccatcgcc gacgacccgt cggccgtggt cgacaacccc gcggggttga gcgggctcgt
     1801 tcagccgtcg ctgctcgcca cctatcaaaa gcccttcggc gtcgacggcc tcgacgaggg
     1861 cttcgtcgcg gtgagcgtcc ccgcgcgtgt gctcaccgtc ggcatggcct ggttccaccg
     1921 cggcctcgac ggcgccctct ccgaggatcg cttcacgctc tcgctcgcgc gcgacttgaa
     1981 gcggacttcc gaggacgcct cgctctcgat cggcgcgagc gtcgacttcg cgcgtgtatc
     2041 cgcggccggc tccatcgacg agaacgatac tgcggtgtcg ttcggcgcgg gcgtgctcct
     2101 gcgccccttc gccttcatcg gcatgggcta caacatccgc agcatcaacc agccctcgat
     2161 cgacttggtc gaggggggag cgagcacgcc gctcgcgcgc gcgcaggcgg cggggctctc
     2221 gtattactgg gacaaccgcc tcacggtgac ggcggagacg ttcgaagacg cgtcgtcgtg
     2281 gcgaacgcgc gccggcgtcg agctgcgact gggcccgcac gtgatgctgc gctgcggctt
     2341 ggagtcctcc cgcgcatcgg cgggtgtggg gatcctgtgg cgcagcgtgg gcttcgacgc
     2401 cgggttcctc tcgcacgagg cgctgggcga gagctacgtg atgacgttcc gctacgcgcg
     2461 aaaggaaccg gcgacgccgt atggatcgac gcgctagcac cgcgacgtat ctgtgcgcgc
     2521 tggccgccgt gtgcctcgcg ggcgacgcgt cggcgttttg gccggtcact tcgcgcgcgc
     2581 gtctcgaact ccgcctgacc gcggacgaag ccagcgcccc ctggtcgtgg gacgcgcagt
     2641 cacgctcgcc tctcgacgac agccgcttca tggtcgacgt cactgcgggc gacgcgcgcg
     2701 tcggaacact ctacgcgaaa ggcgccgcga gctggaacga cgacgccgac gccggggggc
     2761 gggtcacgtt cgcgctcgag cagggcgact atctatttcg atggaaccgt gccgaggtgc
     2821 gcgccttcgg cgacgagcgg cgctttttca cgtacgacct cggcgcggca ctcatggacg
     2881 acgacgtggt cgacgactat cagcatcgta tcggcgtgcg cgccgacggg ggcacgcagc
     2941 gcatcggcgg cacgctcttg gtgtcgagcc tcgaccaagg aagcgacacc caactcgtga
     3001 gctacgccaa gctgcgcacg gggtggaggc cgctcgcggc ggcgctgtcg taccgggtgc
     3061 aggacgacga cgcgaccgat ctggcgatcg ccaagggcga agtggccggc ttctggaagc
     3121 gggccacggt ggtggcgtcg tacgagcagt cgggcgtggg gtcgggcgtg ttcgttcccg
     3181 acggcgactg ggactccttc gggagcggat acgccgccgc cgcgccggag aactcggcca
     3241 ccttcgtcga agcgcgcctc gcgcgcagcc gcatcggaag ctcggcggtg ctgtccggcg
     3301 tgtatcgcta cgcgctcgcg ggtgcggcct acgccaacgg cctcgcctcg ctgcccgccg
     3361 gtaccgagct gcaccggctc gggctgtacc tctcgcaccg ccgctacgcg ctcgacgggc
     3421 gcctcgtgct gttcgacgcc gaccacgcgt acgacgagcg cggggtggcg gcctcggcgc
     3481 gcgcgttcct caaggacaac tccgagatga atctacgcgc gaagatcgtc gacgcgacgc
     3541 gcgacgacgg cgaggagcgc accacgggct ccgcgcacgc gatctaccgt cgctcgctgc
     3601 agcgcttcat gggcggcatc gacgcgctgg tcgacgacat cggcgccgac gccgtcacgg
     3661 cggtcggggt cgagacgcgc atcaactgga gtgcgaccag cgcgctctac ctgcgctgga
     3721 tcgcctcggg cgcggtggaa cgcagcgacg cggtgtacgc gcggctggag tttcggccca
     3781 ccgcgcgcac gtgggtgacc ttcgcgtacg gtcgcgcggt ggcgggagat ggaccctatc
     3841 tcctcgagga cgacgatgcg ctgcccaccg ccggcaccga ggacgtgttc acgatcgtcg
     3901 tgcgggggga cttctagcat gcgcgcgcgc cgcgtcatcg tcgcagcggt agcggtgtgg
     3961 ctcgtgctca ccgctacgca gcgcgcgcgg gccgcggtgc gcgtcgaagc cgacgaggtc
     4021 atcttcacgc tcaccgctcg cgacgccaag gaggccttcc tggtcggcga cttcaatcag
     4081 tggaacccga ccgtcgagcc gatgcagcgc gaaggcgacc agttcgtcgt gcgcttgttt
     4141 ttggtcgcgg ggacgtaccg ctacaagttc gtggtcgacg gcaaggccat cgccgacccc
     4201 gacaaccgcg gcccctcgcc cgaccagggc tcgcccatcg tgctcgtgga gcgaagcggg
     4261 ggactgatct tgagcacgga gatccccgag gaggcggcgc gcgcgcggag cgccagctac
     4321 caggcgcgct acctcgggtt tctgcgcgac gacgacggcg acaccgacgt cgagcagcgc
     4381 gtcgacctcg gcctgcgcgc cgacctggac cgcctgcgcg cgcgcgcgat agtgacgacc
     4441 gccgactcct cgtggacgtg gtcgcccccg agcatcgagg tcgaattcga ccgcggcttc
     4501 gtcgaggtgg agatggggaa gctctccgtg cgcggcttcg agaacgactc gacgtgggcc
     4561 tcgtccggcc cgctgggcct ggtcggccag gcggggttgt accgctacga cgccggcttc
     4621 gcctatcacg gagccaccgc ggtcgccgcc gccaagcacg cggccttgcg cgcgcgctgg
     4681 tccgacgaga ccacgcgcgg cgatctctcg cgcgcgagcg tgccgcccct cgacctctcg
     4741 tcgttcgcga acggcaccgc ggccgccacc accgcctacg cgtacacgcc gaccttcaac
     4801 ggctccgaca acgtcgccgt cgaagccagc gccgacgcgg gcgacgtcgc gtccggctac
     4861 gcgttccgca acgacaccgg cgtcaacccg ggcctgtggg tggacgtggc gcgcgcgggg
     4921 gtcgacttcg cgacccagac atacgcgacc aaagaggatc gtcacgtctc cgcggcgtgg
     4981 ctttcatgga gcggtctcga tcgtaccacc ctgagttttg cctacgggtg gggcgacgcc
     5041 gaggcgcgcg cctacgccac cgcgagcggc accagcgacc tctccgcgcc gctcgatgcc
     5101 gccgccgcga ccacaccggt cgacgtgacg cgcggaattc tcacgagcaa tcgcttcgtg
     5161 gtggaagcga cgtcggcgcg gtcggtggca acgtccctgc gctgggacta tgcgagcttc
     5221 gacttcggcg gtgtggaggg cacgtcgcag gccgacgtgc accgcgcgcg cgccgacgtc
     5281 gcgggcacgc tgcggggctg gagcctctcg ggacgcgtcg cgtacacgca ccagcgctac
     5341 cgcgacaccc ccgacgcgct ctatatcgac tggcccgaac gcaacctgtg gctctcgcgc
     5401 tgggacgcgc tcgatgcgcc ttcgatggtc gccatcgacc tcgagcagca cacggtgtgg
     5461 tcgctcaagg caacgcgcga ggggacgcgc gtcgacgccg ggggggaggc actgttgcag
     5521 acgctggagg tcgtggacgc gcccgtgcac gcggaggtgc gcgcgtacgg cgacgtgacg
     5581 gtgcgcggcc cgtggtatct ctacggcgat gcgcgcgtct cgtggtacga tcgcgccgcc
     5641 tggggcgtgg acgaggcgtt ctgggatttc tacgtcgaag gcggctaccg gaagggtgtc
     5701 gtgaagctca gcgccggctt cggtctcgac ccctgggcct tcgaccccgt gatcagcgac
     5761 ttcgccgacc tggggcgcag cgagttctta cgcgacgcga tcgccggtgg ggtacgccgc
     5821 agcgacgcga ccgcgatcgg gcgcgcgctg gtcgagcgcg agcgggcact ctcggacctt
     5881 cgtttgttca agctggaatg cgtcatcgag ctgcgataag tttaagcgct ctggtcgtgc
     5941 tctcgaccgg gtgcgcatcg ctcgctccgc gcgcgcgcgc gaccgggccg gccgtggccg
     6001 acgacggggt gcgctttcgc ttcttcgcgc ccaccgcgca ccgcgtccag ctcgccggca
     6061 gctggcccga gaacaactgg gccaagggcg acggctccgt gggcgaggcc aacgtcggct
     6121 tgatgtccga cgacgacgcc gacgggatgt gggagatcgt ggtaccgctc tccgccgggc
     6181 gctaccagta cctgttctgg atcgacgagg cgacctggag tctggatcct ggaaaccccg
     6241 aggaggtcca ggctggtcct cagggacgcg cgtcggagat cgtggtcgtg gttcgagacg
     6301 acgaacggga gattcgatga agcccattct ctgggtcggg gtcgcgctca ctctcgccct
     6361 cgcggcctgc ggtggacctc cgccgcccgc cgccaccggg gggcccgagc cggtcgcggg
     6421 tggggccgtg tttcgctacc gaaaccccga tgccaaaaag gtttacctcg tcggcgactt
     6481 caacgactgg tcgcccacct cggaccccat gaccgacgac aacgccgacg gcgagtggac
     6541 gctcttctat ccgctcgtgc cgggcaccta cgcctacaag ttcctggtcg acgggaggaa
     6601 ctgggtggcc gacccgacca accccctctc cgagccggac ggtttcgacg gccgaaactc
     6661 gatcctgaag ctgctcccac ccggctaggc tgggcgataa cccccgaccc cgcagggggt
     6721 ctgggcggtt tcgtcgaccc ggcgttacga ttgcgcccag cagcccccgc ctgctacagt
     6781 agcctccaac cctcttgcga tcgccgaagt ccttgccgtc gttcgtggtg tccagggcat
     6841 tgcaggttaa ttctcgccat cgcccggtag cgtcgagcca tcgatagacc taggtgggcc
     6901 ggggcacagg agttgcagca ttgggggttg ggggattgtc tcctcgaacg gacccgatga
     6961 agacccacag atccatccga atcaccgccc tcgccggcgt gttggccgca accgtcgtcg
     7021 cactcaccgc cgccggtccc gggagcggca ccgcgaacat ttcgccctcc tcgaacgtgg
     7081 ccgcgggatc cgccggcgaa tggagcatca cctacaccgc ggccgaggta cacgacaacg
     7141 ggaccctccg gctcacgatt cccgcgggct ggaccgcgcc ccaggacgca tcggcgatgt
     7201 cgcccggctt cgttgccgtc gtcaccgacg agccgacggg tgcgcccggc ctctccatcg
     7261 cggggcaagt gatcacggtc gtcgtcgaca cgctcaacgt cggcaacacc atcaccatcg
     7321 tctacggcag cgacgacatc agcatgatgg gtcgcgcgtc ggccgcgacc atggtcggct
     7381 cgtacgcgtt cctggtcgaa tcggatccgc tgggcgcttc gcccgcgccc cttgtgacct
     7441 cgccgagcat caacgtcatc gcgaccacgc ccgcttcgtt cgacatcctt cccgacgaca
     7501 ccaccgcgac cgccggcgac ttcgtcgagc tgcacattcg cgtgctcgac accttcggca
     7561 accgcgcgcc ggtttcgagc aatcgcaccg tcaacttgtt cgcgacccac gggcagtact
     7621 tcgacccgtc gaatcattcc acgccgatca cgtcgaccgt gatcgcgaac gggaccaacg
     7681 tcaagcgcgt cgactaccgg cccacactcg tgccgggctc gccgcacacg ctcaacgtgt
     7741 tcaccgcgag cgggtcgccg tcgttgggcg gctccgagtt cgtgcgcgtg acaccggcgc
     7801 cgatgagccc ggtccaatcg ttgatcacgg ccaccacacc cgtggtcgcc aacggctcgg
     7861 cgcagagcac ggtcgtggtg acctcgcgcg atcagttcgg aaaccggcgc cccggtgaca
     7921 cggtgaccat cgatgccacc ggaagcgcgg tcgacgtcga tcctccgcta cccaccgatg
     7981 ccatcgggca gacctccggt gtggtcacga acttggtcgc ggagccggtg acggtgtcgg
     8041 cgcaaatcaa cgcgcagacc atcaccgcga ccgcgcccat ctcgttcacc gcggggccgc
     8101 ccagcggtgc caccagcctc gtcgacgcca ccaccaacgt cgtcgccaac ggtgttgcca
     8161 ccagcgtcat cacggtcacc gtgcgcgacg ccaacaacaa cccagtctcg gggcagaccg
     8221 tcaccctcgc ggtcaacccg gtcccgaacg cgaccctctc gcagccgggt ggcgtgacca
     8281 acgcgctggg tcaggtgacc ggaacgctcg cgagcacgac cgccgtgcag cgaacggtca
     8341 ccgccgtcat cgcgccgagc acgcccatca ccgacaacgc gatcatccag ttcgtcccgg
     8401 gtccgctggc gagcttccaa atcggagtcg acggcaatgc catcgccggc accgtcgatc
     8461 ccgtcacgct aacggcgcgc gacgcgcagg ggaacacggt cacgagctac accggcacgg
     8521 tcaatctcag gaccaacagc ggcgtggcga acagcgtcga atgggccctg ggcgacggca
     8581 acggcatcat caccaatgtt ccgggtagcg acgacggcac gtacaccttc gccgcggccg
     8641 acaacggcgt ggccattatt cgcgtcatcg acgagcgagt cgaaacgatt cagattaccg
     8701 cggacttggg aggcccgccg ctgggcacct ccggaaacct ggtggtgacg aacaacgtcg
     8761 ccgacaagat cgagctggtc gcgggcaacg gccagtcggc gaccgtcaac acgcccgtgt
     8821 ccgttccacc gctcgtgcgc gtcgtcgacg cattcaacaa cccggtgccg acggcgaccg
     8881 tgaccttcac cgcggtcggc gggggcggaa gcgtcgacgt gacatcgggc ggtgcgcccg
     8941 actcgacggg aatcaccggc tcggacggcc gcatcgactg cgatgtttgg cggttgggta
     9001 cgggcgcggg attgaaccgc ctgcgcgcgc gcatcgcgtc cggcaccatc acgagcgtcg
     9061 actt
//
LOCUS       DASPGK010000053         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1301188, whole genome shotgun sequence.
ACCESSION   DASPGK010000053 DASPGK010000000
VERSION     DASPGK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301188"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VEC56_01360"
     CDS             complement(<1..1527)
                     /locus_tag="VEC56_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXV12830.1"
                     /translation="MDPERRDDDARSAHTVDFGREDIEQSIGARFESRARRFPDRIAI
                     RTSTRRVTYREFEARVDAIARAIVAARGPQSEPVALLLNDDLDSVTAMLAVLKSGKFF
                     VVLDPGQPDARLSLILADARAPLVVTDARSRERARALAGAVLDIDAVGAPAAGAPPLP
                     PGSPDARAYIVYTSGSTGQPKGVLQSHRNVLHNTFIHTQFLSITPNDRLTLLSSRGTG
                     QAMTGIFSALLNGAALHPFDLRTRGLTELAAWLVDEKITIYHSSASVFREFADTVLDD
                     MTASSLRAVKLGSEPVSRKDVARFQGRFPPGCVFVNALSSTETGSIRQYRIDHHTRIE
                     GDAVPVGYAVPDKEVVLLAESGARVAIGDVGEICVRSRYLALGYWGDEESTRRSFTPD
                     PGDPEVRLYRTGDLGRMSADGCLVCVGRKDLRVKVRGNRVEVSEIEVALLGLGTVKQV
                     AVVERKDARGHARLVAFIVPARTDEAPRPRALREFLGTKLPRHMVPAAFVFVDELPRT
                     P"
     gene            complement(1517..2464)
                     /locus_tag="VEC56_01365"
     CDS             complement(1517..2464)
                     /locus_tag="VEC56_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HXV12831.1"
                     /translation="MSAPARRVTLRDATLREGLDTPHVSFTTEQRVGVARALVRAGVV
                     EAEVVAPSRVAADLELVGAIRDHGIALECSGLIYASSNDALSEIDGAAHVLDHIDLLM
                     PVSTERAPESRDAKLRALDDALVHAAGRHADVGAGFPHSLQCDPAFLCEIAALAIERG
                     ARRIVVYDTNGGGDPVAVAGLIELLRERVAGANVYFHAHNDLGLATANALAAVAAGAR
                     GLDVTVNGLGDRAGNAALEQVAMALHLKGFETGIHLHELGALSNVVAEASGVAVSKLA
                     PVVGEYVHWHRSPSHLRAPGLFEAFDPALVRSQRKIDGS"
     gene            complement(2461..3639)
                     /locus_tag="VEC56_01370"
     CDS             complement(2461..3639)
                     /locus_tag="VEC56_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV12832.1"
                     /translation="MSTPSSSRFEDCVDGRVDATQSVVLRYRNAFRFGATDDEDARFK
                     DWLAQTSIREGARIVPFLEYQDVLIHVLDETSLMHTRSLKSIDGCFTTARCKSLGYTR
                     TIFETGGNTGTALTAYGTHAGIETFCFVPEDNVSLLDSRMFASPLAHLIAVDKPGRVK
                     RAAHLFARVSDVRHIPQTSWRYEASQFRGCFILEHLLEHGSFDWLVQTISAAFGPIGI
                     YRVLERFRGELGTPPRLLGVQQEANCPMYRAWRSGSSYVVPQTVSSTTKLLSRVMYDN
                     APHTYGTFEDLRKVLTDYRGDLTTVNHEEFAEFLSSELDGSGVLELLADRGIDIAAET
                     IDKTGLIAMAATLREIRRGTIAPGSRVLCCVTSGTMNPDGRVQPDLVIRSRQPSRRAP
                     "
     gene            complement(3623..4165)
                     /locus_tag="VEC56_01375"
     CDS             complement(3623..4165)
                     /locus_tag="VEC56_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXV12833.1"
                     /translation="MEFDSVVRGRRSIRRYRSDAVSESEVRDLVDLARHAPSSMNEQP
                     WHFVLVASADKKARLAEIKNTYCPPDKRHYRADFLRSAAWIVVVCVDRARSHGRAVET
                     AVAATTTLLLAAHARGLGSVFMTAHAPDEPGLASEIRGLLGIPEGIDPVTLLPLGRPG
                     ETPAEKRLAPFASMIHVDTF"
     gene            complement(4217..>4464)
                     /locus_tag="VEC56_01380"
     CDS             complement(4217..>4464)
                     /locus_tag="VEC56_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXV12834.1"
                     /translation="NELPADLPVGPLSAHGADVTTFAFPERFAFLPGVEGPGLPERFR
                     KRALSIPMPGAVESLNAATATAIALYVWSRRTPKTRP"
BASE COUNT          716 a         1480 c         1575 g          693 t
ORIGIN      
        1 cggcgtgcgc gggagctcgt cgacaaaaac gaacgcggcg ggcaccatat gccgcggcag
       61 cttcgtgcct agaaactcgc gcagcgcgcg ggggcgcggc gcctcgtcgg ttcgagcggg
      121 gacgatgaac gccactagcc gcgcgtgacc gcgcgcgtcc ttgcgctcca ccacggcgac
      181 ttgcttgacc gtgccgaggc cgagcagggc cacttcgatc tcggatactt cgacgcgatt
      241 gccccgcacc ttgacgcgca gatccttgcg gcccacgcac accaggcatc cgtccgcact
      301 catgcgtccc aagtcgcccg tgcgatagag acggacctcc gggtcgccgg ggtcgggggt
      361 gaaggatcgg cgcgtgctct cctcgtcccc ccagtacccc agcgcgaggt accggctgcg
      421 cacgcaaatc tcgcccacgt cgccgatcgc gacccgcgcg ccgctctcag cgagcagcac
      481 gacctccttg tcgggcacgg catagcccac cggaaccgcg tctccttcga tacgcgtgtg
      541 gtggtcgatc cgatactggc ggatgctccc cgtctcggtg gacgaaagcg cgttgacgaa
      601 cacgcagccg ggaggaaagc gcccttggaa ccgcgcgacg tccttgcgcg aaaccggctc
      661 gctcccgagc ttgaccgctc gcagcgacga ggcggtcatg tcgtcgagaa ccgtgtcggc
      721 gaactcgcga aagacgctcg cactggagtg atagatggtg atcttttcgt cgaccagcca
      781 cgccgcgagc tccgtcaagc cgcgcgtgcg caggtcgaac gggtgcagcg cggccccgtt
      841 gagcagtgcc gagaagatgc cggtcatggc ctgcccggtc ccacgcgacg agagcagcgt
      901 caaacggtcg ttgggcgtga tgctcaagaa ttgagtgtga atgaaagtgt tgtgcagcac
      961 atttcggtgg ctctgaagaa ctcccttggg ctgtccggtc gaccccgatg tatagacgat
     1021 gtatgcacgc gcgtcggggg aacccggtgg tagcggcggc gcgcctgcgg cgggtgcgcc
     1081 cacggcgtcg atgtcgagta cggccccggc cagcgctcgc gcacgctcac gactgcgcgc
     1141 gtcggttacc accagggggg cgcgcgcgtc ggcgagaatc agcgacaggc gcgcgtcggg
     1201 ctggccgggg tcgaggacga cgaagaactt gcccgacttc aggaccgcca gcatggcggt
     1261 gacggaatcg agatcgtcgt tgagcaggag cgcgacgggt tcggactgcg ggccgcgcgc
     1321 ggcgacgatc gcgcgcgcga tcgcgtcgac gcgggcctcg aactcgcggt aggtcacgcg
     1381 tcgcgtggag gtgcggatcg cgatgcggtc ggggaagcgg cgcgcgcggc tctcgaagcg
     1441 cgcgccgatc gactgctcga tgtcctcgcg cccgaagtcc acggtatgcg cggaccgggc
     1501 gtcgtcgtcg cgtcgttcag gatccatcga tcttgcgctg ggaacgaacc agcgcggggt
     1561 cgaaggcctc gaacaagccg ggcgcgcgca agtggctcgg cgatcgatgc cagtggacgt
     1621 actcgccgac cacgggggcc agcttggaga ccgcgacccc gctcgcctcc gcgaccacat
     1681 tcgacaacgc gcccaattcg tgcaagtgaa tcccggtttc gaagcccttc aggtggagcg
     1741 ccatcgcgac ctgctccagc gcggcgttgc cggcgcggtc gccgagaccg ttcacggtta
     1801 cgtcgaggcc gcgggcgccg gccgcgaccg cggccagcgc gttcgccgtc gccaacccga
     1861 gatcgttgtg cgcgtgaaag tacacgttcg ctcccgcgac tcgctcgcga agcagttcga
     1921 tcaaccccgc caccgcgacc gggtcgccgc caccgttggt gtcgtagacg acgattcgtc
     1981 gcgcgccgcg ctcgatcgcg agggccgcga tctcgcacaa gaacgcggga tcgcactgga
     2041 gggaatgcgg gaagcccgcg ccgacgtccg cgtggcgccc cgccgcgtgg acgagggcgt
     2101 cgtcgagcgc gcgcagcttg gcatcgcgcg actccggcgc gcgctcggtc gacaccggca
     2161 tcaagaggtc gatgtgatcg aggacgtggg ccgccccatc gatctcggag agagcgtcat
     2221 tcgaggaggc gtaaatcagc cccgagcact cgagcgcgat tccgtggtcg cgaatcgcgc
     2281 ccaccagctc caggtccgcg gccacgcggc tgggtgcgac cacctcggct tccaccacac
     2341 cggcgcgcac gagcgcgcgc gcgacgccaa cgcgctgctc cgtggtgaag gagacgtggg
     2401 gcgtgtcgag cccctcgcgc agtgtggcgt cgcgaagcgt gacccggcgc gcgggtgcgc
     2461 tcacggcgcc ctccggctcg gttgccggct gcgtatcacg aggtcaggct gcacgcggcc
     2521 gtcggggttc atcgtgccgc tggtgacgca gcacaggacg cggctcccgg gcgcgatggt
     2581 ccctctgcga atctcgcgca gcgtcgccgc catcgcgatc agtcccgtct tgtcgatggt
     2641 ctccgcggcg atgtcgatgc cgcggtccgc aagcagctcg agcacgccgc tgccgtccag
     2701 ctccgaggac aggaactccg cgaactcctc gtggttgacc gtggtcaaat cgccccgata
     2761 gtcggtgagc accttgcgca agtcctcgaa ggtgccgtag gtatgcggcg cgttgtcgta
     2821 catgacgcgc gacaagagct tcgtggtcga gctcacggtc tggggaacca cgtaggacga
     2881 cccgctcctc catgcgcggt acatcgggca gttcgcttcc tgctggacgc cgagcaggcg
     2941 cggcggcgtg ccgagttccc cgcggaaccg ctccagcacg cggtagatcc cgatggggcc
     3001 gaaggccgcg ctgatggtct gcaccagcca gtcgaagctc ccgtgctcga gcaggtgctc
     3061 caggatgaag cagccgcgga actgcgaggc ctcgtagcgc cacgatgttt gcgggatgtg
     3121 cctgacgtcg ctcacgcgcg cgaacagatg cgcggcgcgc ttgacacgcc ccggcttgtc
     3181 gaccgcgatc aagtgggcca gcgggctcgc gaacatgcgg ctgtcgagga gcgagacgtt
     3241 gtcttcggga acgaagcaga aggtctcgat gcccgcgtgc gtcccgtacg ccgtcagcgc
     3301 ggtcccggtg ttgccgcccg tctcgaagat ggtacgggtg tagcccagcg acttgcaccg
     3361 cgccgtggtg aaacacccgt cgatcgactt gagcgagcgg gtgtgcatga gcgacgtctc
     3421 gtcgaggacg tgaatgagga cgtcctggta ctcgagaaac gggacgatgc gggcgccctc
     3481 gcggatgctg gtctgcgcga gccagtcctt gaagcgcgcg tcttcgtcgt cggtggcgcc
     3541 gaagcggaat gcgttgcggt agcgcaggac gacgctctgc gtggcgtcga cgcggccgtc
     3601 gacgcagtct tcgaatcggg agctagaagg tgtcgacatg gatcatcgac gcgaagggtg
     3661 cgaggcgctt ttcggcgggc gtctcgccgg ggcgccccag cggcagcagc gtgacggggt
     3721 cgatcccttc ggggatgccg agcaagcccc ggatctcgct cgccagtccg ggctcatccg
     3781 gggcgtgcgc ggtcatgaat acgctgccca gtccgcgcgc gtgggcggcc aggagcagcg
     3841 tggtcgtggc cgcgaccgcg gtttccaccg cacgtccgtg cgatcgcgcg cgatcgacgc
     3901 acaccaccac gatccacgcg gccgagcgca ggaaatcggc gcgataatgc cgcttgtcgg
     3961 gggggcagta cgtgttcttg atctccgcga ggcgggcctt cttgtcggcg gaggcgacca
     4021 ggacgaagtg ccacggttgc tcgttcatcg acgagggggc gtggcgggcg agatcgacga
     4081 gatcccggac ttcggattcc gagacggcgt ccgatcggta tctccggatc gatcgccggc
     4141 ccctgacaac gctgtcaaac tccatgcacc acctcgatgc ggctcattga tcttatcacg
     4201 ttgtcacgaa gcgcgcctac ggtcgtgttt tcggggtgcg ccgcgaccac acgtacaatg
     4261 cgatcgcggt cgcggtggcg gcgttcagcg attcgaccgc gcccggcatc gggatcgaga
     4321 gtgcgcgctt gcgaaagcgc tcgggcagac ccggtccttc gacgccggga agaaacgcga
     4381 agcgctccgg aaacgcgaag gtggtcacgt cggcgccgtg cgcggacagc ggaccgacgg
     4441 gcaaatcggc cggcaattcg ttca
//
LOCUS       DASPGK010000054         5290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1535423, whole genome shotgun sequence.
ACCESSION   DASPGK010000054 DASPGK010000000
VERSION     DASPGK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535423"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VEC56_01385"
     CDS             <1..597
                     /locus_tag="VEC56_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV12835.1"
                     /translation="LVVFAADAVTQCPLTLDYGLLTKLVDQVDFGMLDDGTAIGMGLA
                     NACNRMKTSEAKSKVIVLLTDGQNNAGMVDPTTAARVAESLGIKVYTIGVGTRGRAPI
                     PIDDPVFGRRLISVDVDIDEVTLRKIAELTDGRYFRATDRQELQGIYARIDELERTKV
                     ASETFVEYTERFKWLLLPVLGLLILELGLEQTILRETP"
     gene            710..2536
                     /locus_tag="VEC56_01390"
     CDS             710..2536
                     /locus_tag="VEC56_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV12836.1"
                     /translation="MIEFGYPSWLWGLAAAPLVVLMTVVGARYRRRALTSFVGAPLQR
                     ALAPGYSWRRHLAKGLLRAFAVGALFVALAAPRFGSQLVKVERQGIDVVIALDTSLSM
                     LAEDVRPNRLERAKQEIVDVLSTLDGDRVGVVAFAGEAIALCPLTVDYAAALMLVRSV
                     DVYTISEPGSAIAEAIEIATSLFEGAGAGDRAIILVTDGENQEGDPVQAAHAAAEKGI
                     RVFAIGLGSPKGELIPERGSDGSVTGYKKDPRGETVLSRLDEKTLEAVASASKGKYLP
                     ATTEGLEIKVLYDEISGMQKKLIKGEFIERKKERFYIPVAVALACLLLDALVTTRATR
                     RRGERARWLHTGAAAAIMLALFAPPATAKDSVDRGKVRSANKLYKKGDYQDAYRLYKE
                     ALGDTAKPVTDAPGVYYNGGNALYMQKKYDRALDAYQRSYSPDSALTGNMLYNRANAL
                     LKGGHVPEAIESYLQALQYLPDDEDARHNLELALAAKQQQEQQKQQQQQQSGDKGEKQ
                     DKSGESQSDSTRSDSQQNQESKPDSTQQQQQQQQQASPDSSMQEQQPDSSSAPLTEEE
                     LRKLSPEDAARILQALQEREEELQKERRKAAFRRNKRGGKDW"
     gene            2541..4364
                     /locus_tag="VEC56_01395"
     CDS             2541..4364
                     /locus_tag="VEC56_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HXV12837.1"
                     /translation="MRVRLIRAAVLAACVVATTVRADEVAVRATVEPSTVEVGGQAVL
                     TVVVEGKFRRGGSPELPPLPDFDVFESGTSQNFSFVNGQTQSSLTFHYVLSPRKEGAF
                     RIEPIRYAVGDKVYTANAVTLDVVAGGAGIAPGPGPGAQDPGQKGSTPSSDESIFVAA
                     SVDRDTVFVNQQVTWSLGYYTDGRVELLRSPNYSPPEAEGFWVEDLPPQSKYYRTLHG
                     RQYLVSEIKRGYFPTAPGTYRIGEARVDIVIDNINRRGVFDDFFSRRGGGFGEPRTLR
                     TDPLEVVVLPLPSSGKPTGFNGAVASDLVVSTVADKQVVQVGEPINVSVEISGVGNMR
                     TIAPPKLADLPQFKTYESGSSNDSFKKDYVVSGRRKFDFVVVPQVEGSFKIPAVRVPY
                     FDPDLKLYQVAQSAEIPVEVKPGTPEDGRKVIYAGGGDDFEVINRDIRFIHAVPASLP
                     IASRPFYTSPLFIALQALPMLLLGASAVVERRRRRLRSDVGFARASRARREAERKLSE
                     AEHAFRDGNASEGFGRVHAAVFGYFADKMNAPVAGLTTDALEAYLRETHASDEVATSL
                     KSVIDACDAARYAAGAASADNGRETAARARDALIALERGAR"
     gene            4361..5194
                     /locus_tag="VEC56_01400"
     CDS             4361..5194
                     /locus_tag="VEC56_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12838.1"
                     /translation="MRVSLGMVVLAVALSAARAGAQIDADSPAARFERANAAYEAGDY
                     AAAIDDYTRVVEAGVVTPELYYNLGNAYFKDGGLGRAVLWYERARRLSPRDPDVRDNL
                     DLTRSLLRDKQLVATEPRWRRALFAWHRDTTAGESVAAASALYVVLCLLGILFVFRES
                     DAISRLYARLSILSPGRLLGLDKAQDLGLAMGLVLLVCGAFATSAVSKIRAEQARSQG
                     VVVAEEVSVFSGPSRDASVQFKVHEGTLVSVRDARPGWVRVDLPGDLSGWVDETTLDR
                     I"
BASE COUNT          910 a         1766 c         1809 g          805 t
ORIGIN      
        1 ttggtggtgt ttgccgccga cgcggtcaca cagtgcccgc tgaccctcga ctacgggttg
       61 ttgaccaagc tcgtcgatca agtggacttc ggcatgctgg acgacggcac cgccatcggc
      121 atggggctcg ccaacgcgtg caaccgcatg aagacgagcg aagccaagag caaggtcatc
      181 gtgctgttga ccgacgggca gaacaacgcc ggcatggtcg acccgaccac ggccgcgcgc
      241 gtggccgaat cgctcggcat caaggtctac accatcggcg tggggacgcg cgggcgcgcc
      301 ccgattccca tcgacgaccc cgtcttcggc cggcggctga tctcggtcga cgtcgacatc
      361 gacgaggtga cgctgcgcaa gatcgcggag ctgaccgacg gccgctactt ccgcgccacc
      421 gatcgccagg agttgcaagg catctacgcg cgcatcgacg agctcgagcg caccaaggtc
      481 gcctccgaga cgttcgtcga gtacacggag cgcttcaagt ggctcctctt gccggtgctg
      541 gggctcttga tcctcgaact cgggctggag caaacgattc taagggagac cccgtgagcg
      601 cgggtggcgt ccaacgggcg cccacaagcg agggactact agtgcgtatg cgcggtaggc
      661 ccgagcgcag tgggctgttg gacgacagga cccgcgcgaa cggtcgaaca tgatcgaatt
      721 cggatatcca tcctggctgt ggggactcgc ggccgcaccg ctggtggtgc tcatgaccgt
      781 ggtcggcgcg cgttatcgcc gccgcgcgtt gacgtcgttc gtcggggcac cgctgcagcg
      841 agcgctggcg cccgggtaca gctggcgccg ccacctcgcc aaggggctcc tgcgcgcgtt
      901 cgcggtgggc gcgctcttcg tggcgctggc ggcgccgcgc ttcgggagcc aattggtgaa
      961 ggtcgagcgc cagggcatcg acgtggtgat cgcgctcgac acgtcgctct cgatgctggc
     1021 cgaggacgta cggcccaacc ggctcgagcg cgcgaagcag gagatcgtcg acgtgctgtc
     1081 cacgctggac ggcgaccgcg tcggtgttgt cgccttcgcg ggcgaggcca tcgccctttg
     1141 cccgctgacc gtcgactatg cggcggcact catgctggtg cgctcggtcg acgtctacac
     1201 gatctccgag cccggctcgg ccatcgcgga agcgatcgag atcgcgacct ccctgttcga
     1261 aggggcgggt gcgggcgacc gcgccatcat cctggtgacc gacggcgaga accaggaggg
     1321 cgaccccgtg caggcggcgc acgcggcggc ggagaagggt attcgcgtgt tcgccatcgg
     1381 gctgggcagc cccaagggcg agctcatccc cgagcgcggc tccgacggct cggtcaccgg
     1441 gtataagaag gacccgcggg gcgagaccgt cttgagccgg ctcgacgaga aaacgctcga
     1501 agcggtagcc tccgcgtcca agggcaagta tcttcccgct accaccgagg gactggagat
     1561 caaggtcctc tacgacgaga tttcgggcat gcaaaagaaa ctgatcaagg gcgagttcat
     1621 cgagcgcaaa aaagaacgct tctatatccc ggtcgcggtc gcgctcgcgt gcctcttgct
     1681 cgacgcgctg gtcacgacgc gcgccacgcg gcgtcgcggc gaacgcgcgc gctggctcca
     1741 caccggggcg gcggcggcca tcatgctcgc gttgttcgcc ccccccgcga ccgccaagga
     1801 ctcggtcgac cggggcaagg tccgctccgc caacaagctc tacaagaagg gcgactacca
     1861 ggacgcgtac cgtctctaca aggaggcgct cggcgatacc gcgaagccgg tcaccgacgc
     1921 accgggcgtg tactacaacg gcggcaacgc gctgtacatg cagaagaagt acgatcgcgc
     1981 gctcgacgcc taccaacgca gctactctcc cgacagtgcc ttgaccggca acatgctcta
     2041 taaccgcgcg aacgcgctcc tcaaaggcgg gcacgtgccg gaagcgatcg agtcgtacct
     2101 gcaagcgctc cagtacctgc ctgacgacga agacgctcgc cacaacctcg agctggcgtt
     2161 ggccgccaag cagcagcaag aacaacagaa gcagcagcaa cagcagcaat cgggcgacaa
     2221 gggcgagaag caggataagt ccggcgagtc gcagagcgat tcgacccgca gcgactccca
     2281 gcagaatcaa gagtcgaagc cggactcgac ccagcagcaa cagcaacaac agcagcaagc
     2341 cagcccggat tcgtccatgc aggagcagca gcccgactcg tcgtcggcgc cgttgaccga
     2401 agaggagctg cgcaagctct cacccgagga cgccgcgcgc atcctgcaag ccttgcagga
     2461 gcgcgaagaa gaactacaga aagagcggcg caaggccgcg ttccgccgca acaagcgcgg
     2521 cgggaaggac tggtagcgcg atgcgcgtgc gcctgatacg agccgctgtt ctcgcggcgt
     2581 gcgtggtcgc gacgacggtc cgcgccgacg aggtcgccgt gcgcgcaacc gtcgagccga
     2641 gcacggtgga agtaggcggg caggcggtgc tgacggtcgt ggtcgaaggg aaattccggc
     2701 gcggcggctc gcccgagctc ccgccgcttc ccgacttcga cgtgttcgag agcggcacgt
     2761 cgcagaattt cagcttcgtc aacggccaga cgcagtcgtc gctcaccttc cactacgtgc
     2821 tctccccgcg caaagagggc gcgttccgca tcgagcccat tcgctacgcg gtgggagaca
     2881 aggtatatac cgcgaacgcg gtgacgctcg acgtggtcgc gggcggcgcg ggaatcgcgc
     2941 cggggccggg cccgggggcg caggaccccg gacaaaaggg ctccacgccg tcgtccgacg
     3001 agagcatctt cgtggccgcg tcggtcgacc gcgacaccgt gttcgtcaac cagcaggtga
     3061 cgtggtcgct cgggtattac accgacggcc gcgtcgagct cttgcgcagc cccaactact
     3121 cgccgcccga ggccgaaggc ttctgggtcg aagacctccc gccgcaaagc aagtactacc
     3181 gcacgctcca cggccggcag tacctcgtgt ccgaaatcaa gcgcggctac tttccgaccg
     3241 cgcccgggac gtaccgcatc ggcgaggcgc gcgtcgacat cgtgatcgac aatatcaacc
     3301 ggcgcggtgt gttcgacgac ttcttcagcc gccgcggcgg cggcttcggc gagccgcgca
     3361 ccctgcgcac cgatccgctc gaagtggtgg tgttgccgtt gccctcgtcg ggaaagccga
     3421 ccgggttcaa cggcgcggtg gcgagcgacc tggtggtctc gaccgtggcc gacaagcagg
     3481 tcgtgcaggt cggcgaaccc atcaacgtgt cggtcgagat cagcggcgtg ggaaacatga
     3541 ggaccatcgc gccgcccaag ctcgccgacc tcccgcagtt caagacctac gagtcgggct
     3601 cctcgaacga ttctttcaag aaggactacg tcgtctcggg tcgccgcaag ttcgacttcg
     3661 tggtggtccc gcaggtcgaa gggtcgttca aaatccccgc ggtgcgcgtg ccctacttcg
     3721 accccgactt gaagctctac caggtggcgc aatccgccga gatcccggtc gaggtgaaac
     3781 cgggcacgcc agaggacggc cgcaaggtga tctatgccgg cgggggcgac gacttcgagg
     3841 tcatcaaccg cgatattcgt ttcatccacg cggttccggc cagcctgccg atcgcgtcgc
     3901 gtccgttcta cacgagccct ctattcatcg cgctgcaggc gctgccgatg ctgttgctgg
     3961 gcgcttccgc ggtggtcgag cggcgtcgcc ggcgcctgcg cagcgacgtc gggttcgcgc
     4021 gcgcgagccg cgcgcgtcgc gaggcggaac ggaagctctc cgaggccgag cacgccttcc
     4081 gcgacggcaa cgcgtcggaa ggcttcgggc gcgtgcacgc cgccgtgttc ggctacttcg
     4141 cggacaagat gaatgcgccc gtggccgggc tcacgaccga tgcgctcgaa gcgtacctgc
     4201 gcgagacgca cgcgagcgac gaggtggcga cgtctctcaa gtcggtcatc gacgcgtgcg
     4261 acgccgcgcg ctacgcggcc ggcgccgcga gtgcggacaa cgggcgcgag accgccgcgc
     4321 gcgcgcgcga cgcgttgatc gcgctcgaga ggggagcgcg gtgagagtct cccttgggat
     4381 ggtcgtgctc gcggtggcgc tgtcggcggc acgcgcgggg gcacagatcg acgccgactc
     4441 gcccgccgcg cgcttcgagc gcgcgaatgc cgcctatgag gccggcgact atgcggccgc
     4501 gatcgacgac tatacgcgcg tggtcgaagc gggtgtcgtc accccggaac tctactacaa
     4561 cctcggcaac gcgtatttca aggacggcgg cctggggcgc gccgtactgt ggtacgaacg
     4621 cgcccgtcgc ctctcgccgc gcgatcccga cgtgcgcgac aacctcgatc tcacgcgctc
     4681 gctcttgcgc gacaagcagt tggttgcgac cgagccgcgc tggcggcgag cgctcttcgc
     4741 gtggcaccgc gatacgaccg cgggcgaatc ggtggcggcg gcgagcgcgc tctacgtcgt
     4801 tctgtgcctc ttgggcattc tgttcgtgtt tcgcgagagc gacgcgatat cccgcctgta
     4861 cgcgcggctc tcgatcctgt cgcccgggcg attgctgggg ctcgacaagg cccaagacct
     4921 cggcctggcg atgggactcg tgctgctggt gtgcggcgcg ttcgcgacct cggcggtctc
     4981 gaagatccgc gccgagcagg cgcgctcgca gggtgtggtg gtggcggagg aagtgtccgt
     5041 gttcagcgga cccagccgcg acgcctcggt tcaattcaag gtgcacgaag gcacgctggt
     5101 ctcggtgcgc gacgcgcgcc ccggctgggt gcgggtcgat ttgcccggcg acttgagcgg
     5161 gtgggtcgac gagacgacgc tagaccggat ctgacgccca ctcgaagtcg atcgcgatct
     5221 ccgtcgaccc aacgcgcagc acgtcaccgt ggttgagcgc cacgcgacgc gtgcgctggc
     5281 cgttgaccca
//
LOCUS       DASPGK010000055         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_130082, whole genome shotgun sequence.
ACCESSION   DASPGK010000055 DASPGK010000000
VERSION     DASPGK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130082"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VEC56_01405"
     CDS             complement(<1..670)
                     /locus_tag="VEC56_01405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV12839.1"
                     /translation="MLERIGSGTAADVFRVRDRQTGVLRAAKILKPDNAADPKILARF
                     EDEFRILRTLHHPHLPEVYDYGWTEEGGRFLIMELVDGVPLDEYFRANPADIWAILYE
                     LCEALTFVHNHKLLHQDIKPSNILIKRTTAYGPDLPLVKLIDFGLIFKRDAGAEVELV
                     GTPEYVAPEVVRGDASLTRAVDYYSLGATLYELLVGRPPFVGEENEVLRAHLEREPVI
                     EEEEL"
     gene            907..1746
                     /locus_tag="VEC56_01410"
     CDS             907..1746
                     /locus_tag="VEC56_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12840.1"
                     /translation="MSVAVVVPVDSTDAPVDSIETSVGAPYDSLESISTTSVEERISK
                     FTLGAGGGRYYREVYPFGTECSSTQVPFEQEYADVGFEFDHQASRTGHLGVRAGYISG
                     DATLATGAILDGTVDATTIVGERSTWYVNPYFSVEKKGFGIGAGGMLSSNPLLLSGQE
                     DYPVDDDVSLYPTGHVRLGSLSQFYISGHLLEGVPVYSGGGALMAGAGLRMFPSLELY
                     AAYCAEGPYQNEAWLGRVTIDLHRSWVLMTTLRFPTSYQLEPDDEYGMSFGLSYRWYR
                     PGE"
     gene            complement(1757..4438)
                     /gene="acnA"
                     /locus_tag="VEC56_01415"
     CDS             complement(1757..4438)
                     /gene="acnA"
                     /locus_tag="VEC56_01415"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HXV12841.1"
                     /translation="MSADYRQTIRHTKGDVAWYSLPALAAKKGADLARLPYSIRVLLE
                     SALRNHDGFKITDADVERLLAWSPKTAGDVEIAFKPARVVLQDFTGVPAMVDLAAMRD
                     AMRRMGKDYRKINPMVPCDLVVDHSVQVDAWASPDALRINAALEFERNQERYEFLKWG
                     QKAFSNFSVVPPATGIVHQVNLEYLARGVFVHEGVAYPDSLVGMDSHTPMINGLGIVG
                     WGVGGIEAEAVMLGEPINMMMPHVVGFRFTGALRDGVTATDLVLTVTQILRKHGVVGK
                     FVEFFGAGLASMPLPDRATIGNMAPEYGATMGYFPVDEETLRYYRLTGREDDHVAFIE
                     AYTRAQQMFFSAKAPEPEYSEIIEMDLATVEPSIAGPKRPQDRIPVREVREAFDAAMS
                     KPVKEGGYGVEKGARGTAVKVALDGGAELRHGSTVIAAITSCTNTSNPSVMIGAGLVA
                     KKAVERGLHVKPYVKTSLAPGSMVVSEYLEESGLQHYLDELGFEVVGYGCTTCIGNSG
                     PLPDVIVSAIEQGSLVAASVLSGNRNFEGRVHPMTKANFLASPPLVVAYALAGTMEID
                     LDREPLGNDRKGQPVFLRDIWPTSSEVRDVVASVVKAKSFRERYQDVFKGNEQWNAIQ
                     SGSSDLYQWNEKSTYIRQPTFFDGVEGPPKPIAPISGARVLAWLGDSVTTDHISPAGS
                     ISQKSPAAKYLVEHGVAPKDFNSYGARRGNHEVMARGTFANIRLRNKLVPGSEGTWTK
                     CFVDGSEMSIYDAGLKYQAAGVPLIVLAGKEYGSGSSRDWAAKGPMLLGVRAVIAESY
                     ERIHRSNLVGMGILPLQFMEGESELLGLRGDEVFDILDLSEALKPGATVRVKATPASG
                     GAAREFKTRVRIDTPVEVEYYRNGGILQTVLRRLAAA"
     gene            complement(4602..5522)
                     /locus_tag="VEC56_01420"
     CDS             complement(4602..5522)
                     /locus_tag="VEC56_01420"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXV12842.1"
                     /translation="MKKFVLMLGAAAIAIYGTYSLVSALQRPDEVSSGPAVTGGIAIA
                     PPAAFVNTTLQARLEGKRGMGADPGACRWFVNDTEVAGVSASSLEPGHFKKGDRVRVE
                     ASIEGAALVSEPVVIANTPPRITMALAELKDAGGEIMVRISAVDADNDPITYSYEWFK
                     NGAPVPSESGATIDVSRFQKGDNVYASVSASDGEASSSPRKSDSIKLGSNAPKITSTP
                     PQALEEDRRFVYQVRVAQGSGSVKYELTEAPEGMVIDGNGRIEWTVPAHEDRDAKQEH
                     KAVVRVTDSTGGWSTQEFRITTSIQAGLAE"
BASE COUNT          943 a         1852 c         1804 g         1019 t
ORIGIN      
        1 cgagttcttc ctcctcgatc accggttctc gttccagatg cgcccgcaac acctcgttct
       61 cctcgcccac gaatggcggt cgtcccacca acagctcgta cagcgtcgct cccaggctgt
      121 aataatccac cgcgcgcgtg agtgatgcgt ccccacgaac cacttcgggg gcgacgtact
      181 ccggcgtccc gaccaactcg acctcggccc cggcgtcgcg tttgaagatg aggccgaagt
      241 cgatcagctt gacgaggggc aggtcgggcc cgtacgcggt cgtccgcttg atcaagatgt
      301 tcgacggctt gatgtcctgg tggagcaatt tgtggttgtg gacgaacgtg agggcctcgc
      361 acagctcgta gagaatggcc caaatgtccg cggggttcgc gcggaagtac tcgtcgagcg
      421 gcacgccgtc gacgagctcc ataatgagaa accgcccgcc ctcctcggtc cacccatagt
      481 cgtagacctc gggaaggtgg ggatgatgga gggtgcgcag gatgcggaac tcgtcctcga
      541 aacgcgcgag gatcttgggg tcggcggcgt tatcgggctt gaggatcttc gcggcgcgaa
      601 gcaccccggt ctggcggtcg cgcacgcgaa acacgtcggc cgccgtaccc gaaccgatcc
      661 gctcgagcac gtcgtagcga tcccagccga gaactcgctg gacgggggca gaggacgcta
      721 atgccataat ggataatatg ttacaacgtg tcgtgcgcgg ccgggttgcg acgggtctcg
      781 caagggtgct agcctttcga cgccagccgg gctggcccga tggtacatcc tagacgcaga
      841 caattgaagc cctccgccgc tgggcggcgc tcgacgggat ggctctgcta cctgctggca
      901 gcgccgatga gcgtcgcggt cgtcgtcccc gtcgatagca cggacgcccc cgttgattcg
      961 atcgagacct cggtcggagc gccgtacgat tccctcgaaa gtatctccac cacgtcagtc
     1021 gaggagcgca tctcgaagtt caccctcgga gccggcggtg gtcgctacta ccgcgaagtc
     1081 tatcccttcg gcaccgagtg cagctccacc caggttccct tcgagcagga gtacgccgac
     1141 gtcgggttcg agttcgacca tcaagcgtcg cgcaccggtc atctcggtgt tcgcgccggc
     1201 tacatctcgg gcgacgcgac gctcgccacc ggcgcgatcc tcgacggcac ggtcgacgcg
     1261 acgacgatcg tgggtgagag aagcacgtgg tacgtcaatc cctacttctc ggtcgagaag
     1321 aaaggcttcg gaatcggagc cggtggaatg ctttcctcga acccactctt gttgagtggc
     1381 caggaggact acccggtcga cgatgacgtc tcgctctatc ccacggggca cgttcgtctc
     1441 ggcagcctat cgcagttcta catctcggga catttattgg agggcgtccc ggtctactcg
     1501 ggtggcggag ccctgatggc gggggccggt ctgcgaatgt tcccttctct tgagctctac
     1561 gcagcctatt gtgcggaagg accgtatcag aacgaagcct ggctgggtcg tgtgacgatc
     1621 gatctccatc gaagctgggt tctgatgacg acgctccgct tccccaccag ctaccagctg
     1681 gaacccgacg acgagtacgg aatgtcgttc gggctttcct atcgctggta tcggccgggc
     1741 gagtagttcg cgccgattac gcggccgcga gtctccgcaa caccgtctga agaatcccgc
     1801 cgttgcggta gtactccacc tcgaccgggg tgtcgatgcg cacgcgcgtc ttgaactcgc
     1861 gcgcggcgcc accggatgcc ggagtggcct tgacgcgcac ggtggcaccg ggcttcagcg
     1921 cctccgagag atccagaata tcgaagacct cgtcgccgcg aagcccgaga agctcgctct
     1981 cgccctccat gaactggagc ggcaggatgc ccatgccgac caagttgctg cggtggatgc
     2041 gctcgtacga ttcggcgatg acggcgcgaa caccgaggag catggggccc ttcgcggccc
     2101 agtcgcgcga ggagccggac ccgtactcct tgcccgcgag cacgatcaac ggcacgcccg
     2161 ccgcctgata cttgagcccg gcgtcgtaga tcgacatctc gctcccgtcg acgaagcact
     2221 tggtccacgt cccctcggag ccgggcacga gcttgttgcg caggcgaatg ttggcgaagg
     2281 tcccgcgcgc catgacctcg tggttgccgc gtcgcgcgcc gtacgagttg aagtccttgg
     2341 gcgcgacacc atgctccacg aggtacttcg ccgcgggcga cttctgcgat atcgatcccg
     2401 cgggcgagat gtgatctgtg gtcacggaat cgcccagcca cgccagcacg cgcgcgccgc
     2461 tgataggcgc gatgggtttg ggcggtcctt ccacaccgtc gaagaaggtc ggctggcgga
     2521 tatacgtgct cttctcattc cactggtaca ggtcggagct cccgctctgg atggcgttcc
     2581 attgctcgtt gcccttgaac acgtcctgat atctctcgcg gaacgacttc gctttgacga
     2641 cactggcgac gacgtcgcgc acctcgctcg aggtgggcca gatgtcgcgc aggaacacgg
     2701 gctggccctt gcgatcgttg cccaacggct ctcgatcgag gtcgatctcc atcgttcccg
     2761 ccagggcata cgcgaccacc aacggcggac tcgcgaggaa gttggccttg gtcatggggt
     2821 gcacgcggcc ttcgaagtta cgatttccgc ttagcacaga ggcggccacc agcgagccct
     2881 gctcgatggc cgatacgatc acgtcgggca gcgggccgct gttgccgatg cacgtggtgc
     2941 agccgtagcc gaccacttcg aagccgagct cgtccaaata atgctgcagg ccgctttcct
     3001 ccaggtactc gctcacgacc atcgaaccgg gcgcgagcga cgtcttcacg tacggcttca
     3061 cgtgcagccc gcgctcgacc gccttcttgg ccaccaaccc cgcgccgatc atcaccgacg
     3121 gattggaggt gttggtgcac gaggtaatgg cggcaatgac ggtcgagccg tggcgcagct
     3181 cggcgccgcc gtcgagggcg accttgaccg cggtaccgcg cgcgcccttc tcgacgccgt
     3241 aaccgccttc cttgaccggc ttcgacatgg ccgcgtcgaa ggcctcgcgc acctcgcgca
     3301 cgggaatgcg atcctgcggg cgcttgggcc ccgcgatcga cggctccacg gtggcgagat
     3361 ccatttcgat gatttcggag tactcgggct cgggtgcttt cgcggagaag aacatctgct
     3421 gcgcgcgcgt gtacgcctcg atgaacgcga cgtgatcgtc ctcgcggccg gtgagccggt
     3481 agtacctcag agtttcctca tcgaccggga agtaccccat ggtggcgccg tactcggggg
     3541 ccatgttgcc gatggtcgcg cgatccggca gcggcatgga cgcgagtccg gcgccgaaaa
     3601 actcgacgaa cttgccgacc acgccgtgct tgcgcaggat ctgcgtcacc gtgagcacga
     3661 ggtcggtcgc ggtgacgcca tcgcgcagcg caccggtgaa gcgaaaaccg accacgtgcg
     3721 gcatcatcat gttgatgggt tcgcccagca tgacggcttc ggcctcgata ccgcccacac
     3781 cccaacccac gatccccagc ccgttgatca tcggcgtatg gctgtccatt ccgaccaacg
     3841 agtcggggta cgcgacgcct tcgtgcacga acacaccgcg cgcgaggtac tcgagattca
     3901 cctgatggac gatgcccgtc gccggcggca ccacgctgaa attggagaac gccttctgcc
     3961 cccacttgag gaactcgtag cgctcctggt tgcgctcgaa ctcgagggcg gcgttgatgc
     4021 gaagcgcgtc gggcgaggcc cacgcatcca cttgcacgga gtgatcgacc acgaggtcgc
     4081 agggaaccat cgggttgatc ttgcggtagt ccttgcccat gcggcgcatg gcgtcgcgca
     4141 tggcggccag gtcgaccatg gcgggcacac cggtgaagtc ctgcagcacc acgcgcgcgg
     4201 gcttgaacgc gatctcgacg tcgcccgcgg tcttggggct ccacgcgagc aagcgttcga
     4261 cgtcggcgtc ggtgatcttg aacccgtcgt ggttgcgcag ggcactctcg aggagcacgc
     4321 ggatcgaata cgggaggcgc gccaggtcgg cgcccttctt cgcggccagc gcgggcaggc
     4381 tgtaccacgc gacgtcgccc ttggtgtggc gaatcgtctg tcgatagtcg gcggatatga
     4441 gtggcaaggt tcctccttgg tgagcgaacc gcaaagatag cacgcgacgc cgttattgtg
     4501 aaggcgcggc ccccgcccgg aaacgaagac gccgcgcagc cgcccttggg gacgacacgc
     4561 gcggcgtttc gattcctacg cgcttgctct gccagagaaa actactcggc caaacccgcc
     4621 tggatggagg tcgtgatgcg gaactcctgg gtggaccagc cgccggtgga gtcggtgacg
     4681 cgaacgactg ctttgtgctc ctgcttggcg tctcggtcct cgtgcgcggg gacggtccat
     4741 tcgatgcggc cgttcccgtc gatgaccatc ccctcgggcg cttccgtcag ctcgtacttc
     4801 acagagcccg agccctgcgc caccctcacc tgataaacga agcggcgatc ctcttcgagc
     4861 gcctgcgggg gcgtcgacgt gatcttgggc gcgttgctcc cgagcttgat cgagtcgctc
     4921 ttgcgcggcg acgaggaggc ctcgccgtcg gaggcggaca cgctcgcgta cacgttgtcg
     4981 cccttctgga accgggagac gtcgatggtg gctccgctct cgctcgggac cggcgcgccg
     5041 ttcttgaacc actcgtagct gtaggtgatg ggatcgttgt ccgcgtcgac cgccgagatg
     5101 cggaccatga tctcgccgcc ggcgtccttg agctccgcca acgccatcgt gatccggggc
     5161 ggcgtgttgg cgatgacgac gggctcgctc acgagcgccg cgccttcgat ggaggcttcg
     5221 acacgcacgc ggtcaccctt cttgaagtgg ccgggctcga gactcgacgc gctcacaccc
     5281 gccacctcgg tgtcgttgac gaaccagcgg caggcacccg ggtccgcgcc catgccgcgc
     5341 ttgccttcca agcgggcttg cagcgtcgtg ttgacgaacg ccgccggggg cgcgatggcg
     5401 atgccgcccg tgaccgcggg acccgacgaa acctcatcgg gcctttggag cgcgctcacg
     5461 agcgagtacg tgccgtagat ggcgattgcc gcggcaccca acatcaacac gaacttcttc
     5521 acgatggcct ccttcgaaca ccggcacaaa agcgaacgcc tccccccgtg cgggaggagg
     5581 gttcgctgcc cttagctcaa ctctggcggc gccggcac
//
LOCUS       DASPGK010000056         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1184222, whole genome shotgun sequence.
ACCESSION   DASPGK010000056 DASPGK010000000
VERSION     DASPGK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184222"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VEC56_01425"
     CDS             <1..698
                     /locus_tag="VEC56_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV12843.1"
                     /translation="YSMIAPTLADSTVAAGQHLSVFFLRAATAAPDIYFDSPTGSGYS
                     LDNLAPGVPTNFAYASSMLSWDESNAADFDYFAVYGSNTNSFASAIVVDYTTGTSMDV
                     SSASYAYFFLTATDFSGNESAPAVLQAPTDVGDAPKRYVLSLSSYPNPFNPATTVSYT
                     VPSRGLVTVAIYDAHGALVTTLVNNEERAPGAYRIGWDGRNDRGASVTSGVYFARIEH
                     GGAARSRKMVLLK"
     gene            710..1579
                     /locus_tag="VEC56_01430"
     CDS             710..1579
                     /locus_tag="VEC56_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV12844.1"
                     /translation="MRTLLLAIFLFGSAGVSVSEPQTGWELVVLGIAQDGGMPHAGCS
                     KPPCAEVHAGTRTAEKVSCLGLVNAESGAAYLFDATPDFPAQLHALTGGRVPDGIFLT
                     HAHIGHYTGLMYLGKESMAAKSVPVYGTARMTKFLETNGPWSLLVKDNYIAPRVLAPD
                     VAVELPGGVRVTPMLVPHRDEFTDTVGFLIEGPRAKALFIPDIDKWEKWNRSLRELAE
                     GVDYLLLDGTFASLEELPGRDISQVPHPLMSETRALLEGTRAKLWFIHLNHSNPAIVE
                     GKDVVREGMRFEL"
     gene            complement(1576..1887)
                     /locus_tag="VEC56_01435"
     CDS             complement(1576..1887)
                     /locus_tag="VEC56_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12845.1"
                     /translation="MVRSAKQKYTGTKRTASVAVASKSNGSSTKARSGRARFVVCLDN
                     SGYDASLDVRKIYRAVPDDKADKLGLVRVVDESGEDYLYPKKLFATIEVSAALRRRLA
                     I"
BASE COUNT          418 a          784 c          687 g          429 t
ORIGIN      
        1 agtacagcat gatcgccccc acgctcgcgg actccacggt cgcggcgggc cagcacttat
       61 ccgtgttctt cctccgcgcc gcgaccgccg ctccggacat ttacttcgac tccccgaccg
      121 gcagcggata ctcgctcgac aatcttgctc ccggggttcc gaccaacttc gcgtatgcgt
      181 cgtcgatgct ctcgtgggac gagtcaaacg cggccgactt cgactacttc gcggtctatg
      241 ggtcgaacac gaactcgttc gcgtcggcca tcgtggtgga ctacaccacc gggaccagca
      301 tggacgtgag cagcgcgagt tacgcctact tcttcctcac cgccaccgac ttctccggca
      361 acgagagtgc gcctgcggtc ctgcaagcgc cgaccgacgt gggtgatgcg cccaaacgct
      421 acgtcttgtc gttgagcagt taccccaacc ccttcaatcc cgcgacgacg gtgagctaca
      481 ccgtcccgtc gcgcgggctg gtgaccgtcg cgatctacga cgcgcacggc gcgctcgtca
      541 ccaccctcgt gaacaacgaa gaacgcgcac cgggcgcgta ccggatcgga tgggatggtc
      601 gcaacgaccg cggggcgtcg gtcacgtcgg gggtctattt cgcgcgtatc gagcacggcg
      661 gagccgcgcg ctcgaggaag atggtgctgc tgaagtagca aggttgctgg tgcgtacgct
      721 cctacttgcg atcttccttt tcggctctgc cggtgtgtcg gtgtccgagc cgcaaaccgg
      781 ctgggagctc gtcgtcctcg gcatcgcgca ggacggcggc atgccgcacg ccggttgttc
      841 gaagcccccg tgcgccgagg ttcacgcggg cacgcgcacc gcggagaagg tgagctgtct
      901 cggtcttgtg aacgccgaat ccggtgcggc ctatctcttc gacgcgaccc cggacttccc
      961 cgcccaactc cacgccctaa ccggcggacg cgtgcccgac gggatctttc tcacccacgc
     1021 gcacatcggc cactacaccg gcttgatgta cctcggcaag gaatccatgg cggccaagtc
     1081 cgtccccgtc tacggcaccg cgcgcatgac gaagttcctc gagacgaacg gcccgtggag
     1141 cctgctcgtc aaagacaact acatcgcgcc gcgcgtgctc gcaccggatg tcgcggtgga
     1201 gttgccgggt ggggtgcgcg tgaccccgat gctggtcccg caccgcgacg agttcaccga
     1261 caccgtgggc tttttgatcg aaggcccgcg cgcgaaggcg ctcttcattc ccgacatcga
     1321 caagtgggag aaatggaacc ggagcctgcg cgagctggcg gaaggcgttg actatctgct
     1381 gctggatggg acgttcgcgt cactcgaaga attgccgggg cgcgacatct cgcaagtccc
     1441 gcatcccctg atgagcgaaa cgcgcgcgct gctcgaaggc acgcgcgcca aactatggtt
     1501 catccacctg aaccattcca acccggccat cgtggaggga aaagacgtgg tccgcgaggg
     1561 gatgcgcttc gaactttaaa tcgcgagccg gcggcgaagg gcggcgctga cttcaatcgt
     1621 ggcgaagagc ttcttcggat acagatagtc ttctcctgac tcgtcgacca cccgaaccaa
     1681 cccgagcttg tcggctttgt cgtcgggtac ggcgcggtag atcttgcgga catcgagcga
     1741 cgcatcgtag cccgagttgt ccaggcaaac gacgaagcgg gcgcgccccg accttgcctt
     1801 agtcgaggat ccgtttgatt ttgaggccac ggcgaccgat gccgtgcgct tcgtaccagt
     1861 gtatttctgc tttgcggagc gaaccatcct gcaacctcac aagcgcaaca cccttgagct
     1921 ttctccagcg cccttctccg aacttcctgc gcaggcgggc aatgtcgcgg atcgcggcac
     1981 ctgccgcgat gacttccacg tcgtgaattc gtccaacgat ctcgaagaac atccacccac
     2041 cacccaaccc aacccagtca actcattcta tgaaatcgtg ccagcaagcg ccagcgggcc
     2101 ctggcaaaaa aagaggggcg ggccccggcc cgcccccccg attgggtacc tggaatgaac
     2161 ggcggactag cgtgcgccgg ccttgtggat cgtcgatgag accatttcca tggtctcgac
     2221 cttcggcgtc ccttccacga ctgtcgccaa cagcttgacg acatccttat agccggagag
     2281 atcgtacgct tccgcgtctt ccttccggtc ccacatgc
//
LOCUS       DASPGK010000057         9590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_599256, whole genome shotgun sequence.
ACCESSION   DASPGK010000057 DASPGK010000000
VERSION     DASPGK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599256"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="serS"
                     /locus_tag="VEC56_01440"
     CDS             <1..718
                     /gene="serS"
                     /locus_tag="VEC56_01440"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXV12846.1"
                     /translation="VEQGYVEVSIPFLVHRASMTGTGQLPKFEVDMYRATEDDLFLIP
                     TAEVPVTNLHRESVLERERLPIKYCAYSPCFRREAGSHGADTRGLLRVHQFDKVEMVK
                     FVHPDSSYDELESLTTDATAVLDALELPYRVVALCAGDLGFASAKTYDLEAFAPGVDK
                     WLEVSSCSNFEDFQARRAEIRLKKSEGEKHKFVHTLNGSGLALPRVVASLLEVHQTPT
                     GRVRIPARLRPYMRGKEYLG"
     gene            722..1969
                     /locus_tag="VEC56_01445"
     CDS             722..1969
                     /locus_tag="VEC56_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV12847.1"
                     /translation="MASASGGSIRFWLKGYLFLGVAVLSLAMLLYSNHLIARMRENSE
                     ATSRLFSRYMANVIFEVTDDGRMADLREVINESDLPIIITVLDGIPILWSNVPVEERT
                     AADLDMLEFVDIDNPPNEKFRKLVALYRDYDKKNDPIPIRVVGATEPSGWVHYGPSPL
                     QRELRYMPFVLLGIFLVFMAVAIQGLRYLKLSEQRSIWVGLAKETAHQLGTPLSAMLG
                     WVQVIKDRAASKGYDDIRSSVEEMEVDLGRLNKVTDRFSKIGSTPDLVEVDLKRVLEN
                     TSAYFQKRLPSLKSKSAISLHVGDIARVRANEELLEWVFENLIKNAIDALGEAGGQIE
                     IRASDDASKKFVDVTVHDTGRGIPGALRDDVFRPGFTTKRRGWGLGLALTRRIVEEYH
                     GGSIKLVESRPGKTTFLVRLPAA"
     gene            2088..2918
                     /locus_tag="VEC56_01450"
     CDS             2088..2918
                     /locus_tag="VEC56_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12848.1"
                     /translation="MVHGFVHDISRDEVPAQDFMPGDQRSDATINVIVLEIEIRHVLK
                     GYHVGSHFMDVVVVQGIPWLRTDYNVGNEVVLSTIYWKYMRGGSYMVMNDGGCFRKVG
                     DTWWNQLVIAPNAPLAVAYSEIEMAAARCLPHVILGQASLAVIGTVDGISPHRKSTST
                     PSENARQIPARNDPRADVIMRVTDVVKGTPGGDTVTFRPVPTDDHARAAKENVPSFAV
                     GDQWLVFLGRDATGYFAIDCTNGLFRVKGDSLIQADRVVLPATKSTFIAEMRKADAAR
                     "
     gene            3011..4558
                     /locus_tag="VEC56_01455"
     CDS             3011..4558
                     /locus_tag="VEC56_01455"
                     /inference="COORDINATES: protein motif:HMM:NF020249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HXV12849.1"
                     /translation="MANKRILWVDDEIELLKSHVMFLRDKGYDVQTATNGTDAVNLVK
                     EGRFDIMLLDESMPGRGGLETLVEIKESDPVLPIVMITKNEEERLMDNAIGMKIDDYL
                     LKPVSPLQIYSACKRILDAPKIQENRLSPDYIREFNEINSLAQEGTWDAWLRVHRRLC
                     AWDREFDQFRNTGLEATHDDQRLQTAHHFGLFVEKNYRDWVASDKRPPMSLDVFPRFI
                     APHLLKGEKLAFILVDCVRLDQWMAIEDFLSEQFDVQWDFYCSILPTATPYSRNSIFS
                     GMFPDDIARRYPDKWLERVNEDSSKNKFEAFFMGEQMKKLKLDESKMRYMKIHTAQEA
                     GDARKRVASMMGSPFVSLVFNFVDILTHGRNQSDILQQLLPNESAFRSLMRSWYSHSV
                     LRDVLADLARAKVKVVLTTDHGSILGRKAALVYGRRDTSTNLRYKFGDNLKCDERQAI
                     ITRKPSEFRLPSESGTKTYLFAKEYFYFVYPTNFRDYEKAYEGSFQHGGVSLEEMILP
                     CLTLTPR"
     gene            4576..5043
                     /gene="tsaE"
                     /locus_tag="VEC56_01460"
     CDS             4576..5043
                     /gene="tsaE"
                     /locus_tag="VEC56_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXV12850.1"
                     /translation="MSRAIAQTVRTNDAAETERFGAALGACIHRGAGVCLTGPLGAGK
                     TVLVRGLCRGLGAVEPVTSPTFILMESFAGRLPIVHIDLYRLEHEREVEDIGVFDMLD
                     GETVVIAEWGERSPALLEECDLEIRIEPGEGTTRTIHVHATPDFARELEGLSW"
     gene            5037..5753
                     /gene="tsaB"
                     /locus_tag="VEC56_01465"
     CDS             5037..5753
                     /gene="tsaB"
                     /locus_tag="VEC56_01465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXV12851.1"
                     /translation="MVTIAIDTSHPVGTTALARDGVVLGEARFDSPSSHLVELSKAIE
                     RLLASSNLTMRDVDRIAVVLGPGSFTGVRIAVSFAKGLAAAGAQVVAMDSLRLLALPF
                     FDQGRARVCAMIDAKRGEVYGALFERASGSVEAVVAPSAGSAAKLLEAIATAPDVFAG
                     TGALAHRDEILARFPRAEVAGEARANPSTTYFASIAHHLPALARDAVRALEPVYLRAS
                     GAERKRLRAHAPEPGASGDD"
     gene            5746..6207
                     /gene="rimI"
                     /locus_tag="VEC56_01470"
     CDS             5746..6207
                     /gene="rimI"
                     /locus_tag="VEC56_01470"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXV12852.1"
                     /translation="MTDQPAFVLVPLEHQHIPDVLKIEREAFPAPWTEAMFRQEVEEK
                     WLSRSFVALLGEEIVGYVIAWFLRGEVHVLNLAVTKAHQRQGLARAMLRHVIGLASEE
                     ACHLITLEVRVSNDPAKLLYLSMGFAPVGIRRRYYHDNDEDALVMTKRLRG"
     gene            6204..6965
                     /locus_tag="VEC56_01475"
     CDS             6204..6965
                     /locus_tag="VEC56_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12853.1"
                     /translation="MSAAATRSLAFIAGSGMDPIAIAMTVGRATRFDQIAGVGAPGVE
                     GHTGRVLEGTIGGGACTLVMGRRHVYEGDPAAVAHLVAWLAERGVTDLVMASAAGALH
                     RGLAAGDLMVAHDIVDFQNRDRLMRVAGSAMRTASAERLRIDRGLSAAIERAATAARV
                     SWQRGIMVCGVGPAYETQAEVLALQEWGDAATMSSAPELAAASRLGLRAAAVAVITNP
                     CTGVASWRPNHAEVLDVGRKMAVGLAEVIRQLVID"
     gene            7089..7988
                     /gene="accD"
                     /locus_tag="VEC56_01480"
     CDS             7089..7988
                     /gene="accD"
                     /locus_tag="VEC56_01480"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXV12854.1"
                     /translation="MSWFRRERRGVQPIQKKAIPDGLWKKCDLCGEILYVKELEKRLW
                     TCTKCGNHFMISTQQYIDILADPGSFRETHTGLISGDPLQFKDLKKYKDRIREYHEKT
                     GMEDAMVTGFASMDGRDVVLAIMDFSYMGGSMGSVVGEKFARAAQDAMLRRRPLIALS
                     RSGGARMQESIFSLMQMAKTAAMLSRMSEEGLPFISILTNPTTGGVTASFASLGDVIV
                     AEPKALIGFAGPRVIQQTIRQDLPEGFQRSEFLLEHGMIDMIVSRDELRRTLAGVLDF
                     MCARAASAPDQEVKDDSQSPAVS"
     gene            7960..9333
                     /locus_tag="VEC56_01485"
     CDS             7960..9333
                     /locus_tag="VEC56_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXV12855.1"
                     /translation="MTRSRRPFPDSPVALPPNLQFVLALSPSVMTLGLDHMRALMERV
                     GHPERRFRSVLVAGTNGKGSTTTMLAAILGAHGLRTGRYTSPHVFNVAERITIDGAHA
                     SIEEMEVAAARLVPLRDEIPFSYFEALTAIAFMVFAARGVEVAVLEVGLGGRLDATNI
                     VEPDVSIVTSISLDHRRILGDTEEQILVEKLGVSRPGVPLLVGPLPDALRAVVNDRAK
                     RDGFPVFDLENIGRVSQVEEAFDGIAFRVETPHAEYGRVSIPFAGSHQVTNALLAIGA
                     AERMLPALVGLGGALARAYIPGRFERVERGGRSFVIDVAHNEASLVATADHLAAFRPR
                     DECAIVFGLMHRKELFEAPRHLLAAASCLCLIEPPSEPGSTDNAFAPHELWARYFAPL
                     LPDSATNVMLWNPSGPRADAMRRLVRWLDRSRYSTVVATGSHRVVEDFGLLLQTSTVE
                     EGGNTSK"
     gene            9330..>9590
                     /locus_tag="VEC56_01490"
     CDS             9330..>9590
                     /locus_tag="VEC56_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXV12856.1"
                     /translation="MSAWIGIDIGGTNIKIATISAHGEVFERGVVETQPADGPERAFA
                     RIHAAARYLAAHRSIQGVGIGCAGLIDSRRGILRESPNLPAWR"
BASE COUNT         1674 a         3154 c         3092 g         1670 t
ORIGIN      
        1 cgtcgagcag ggctacgtcg aagtgtcgat tccgttcctc gtgcaccgcg cgtcgatgac
       61 gggcaccggg caattaccca agttcgaagt cgacatgtac cgcgccaccg aagacgacct
      121 ctttctgatc ccgaccgccg aagttccggt gaccaatctc catcgcgagt cggtcctcga
      181 gcgcgagaga ttgcccatca agtactgcgc ctacagcccc tgtttccgcc gcgaagccgg
      241 gagccacggc gccgacacac gcgggctgtt gcgcgtgcac cagttcgaca aagtggaaat
      301 ggtgaagttc gtgcaccccg attcgtcgta cgacgagttg gagtcgctca ccaccgacgc
      361 caccgcggtg ctggatgcgc tggagctccc gtaccgcgtg gtggcgctgt gcgcgggcga
      421 cctgggcttc gccagtgcca agacctacga cctggaggcg ttcgcgcccg gcgtcgacaa
      481 gtggctcgag gtctcgtcgt gcagcaactt cgaggacttt caagcgcggc gcgcggaaat
      541 acgattgaag aagagcgaag gggagaagca caaattcgtc cacacgctga acggctcggg
      601 actcgcactc ccgcgcgtgg tagcgtcgct tctggaagtg caccaaaccc cgaccggccg
      661 cgttcgcatc ccggcgcgct tgcgccccta catgcgcggc aaggagtacc tgggctgacg
      721 gatggcgtcg gcgtccggcg gtagcattcg tttctggctg aagggatacc tgttcctcgg
      781 cgtggccgtg ctcagcctgg ccatgctgct ctattccaac catctcatcg cgcgtatgcg
      841 cgagaactcc gaggccacca gccgcctgtt ctcgcgctac atggccaacg tcatcttcga
      901 agtgaccgac gacggccgca tggccgactt gcgcgaggtg atcaacgaga gcgatctccc
      961 catcatcatc accgtgctcg acgggattcc cattctatgg agcaacgtgc cggtcgaaga
     1021 gcgcaccgcc gccgacctcg atatgctcga gttcgtggac atcgacaacc cgcccaacga
     1081 gaagtttcgc aagctggtgg cactctaccg cgactacgac aagaagaacg accccatccc
     1141 gattcgagtg gtgggcgcca ccgagccctc cggctgggta cactacggcc cctcgccgct
     1201 ccagcgcgaa ttacgctaca tgcccttcgt cctgctcggc atctttttgg tgttcatggc
     1261 ggtcgcgatc caggggctgc gttatttgaa gctaagcgag cagcgttcca tctgggtcgg
     1321 tctcgcaaag gagaccgcgc atcagttggg cacgccactc tccgcgatgc tcggctgggt
     1381 gcaggtgatc aaggaccgcg ccgcgtcgaa gggctacgac gatattcgtt cgtcggtcga
     1441 agagatggaa gtcgacctcg ggcgtttgaa caaggtcacg gatcgtttca gcaagatcgg
     1501 ctccacaccg gacctagtcg aagtggactt gaagcgcgtg ctcgagaaca catcggcgta
     1561 tttccagaag cgactcccca gcctcaagtc gaagtccgcc atttcgctcc atgtcggtga
     1621 catcgcgcgc gtgcgcgcca acgaagaact gctcgagtgg gtgttcgaga acctgatcaa
     1681 gaacgccatc gacgccctcg gcgaagccgg cgggcaaatc gaaatacgcg cctccgacga
     1741 cgcctcgaag aagttcgtcg acgtcaccgt ccacgacacc ggccgcggca tccccggcgc
     1801 actgcgcgac gacgtgttcc gccccggctt caccaccaaa cgccgcggct ggggcctcgg
     1861 cctcgccctc acccgccgca tcgtcgagga gtaccacggc ggctcgatca aattggtgga
     1921 atcacgaccc ggcaagacga cgtttctcgt ccgattgccc gcagcttgac aaagcggcgc
     1981 atctctctcc tgtccgtgct gggcgtactt gcgatcgcgc ccgtcgttgc gccacgcgcg
     2041 caatctgatc caccacccca catcgatttg atcgagcatt cgacctcgtg gttcacggtt
     2101 tcgtccacga catttcgcgt gacgaagtgc ccgcgcaaga tttcatgccc ggcgaccagc
     2161 gatccgatgc aacgataaac gtcatcgtac tcgagatcga aattcgccac gtgctaaagg
     2221 gatatcacgt cggatcgcac tttatggatg tggtagttgt tcaaggaatc ccctggcttc
     2281 ggaccgacta caacgttggg aatgaagtcg tactgtcgac catctattgg aagtacatgc
     2341 gcggcggatc gtacatggta atgaacgacg gcggctgttt ccgcaaggtt ggagacacgt
     2401 ggtggaatca gttggtgatt gcgccgaatg ctccactcgc agtcgcatat tcggagattg
     2461 agatggcagc tgcgcgctgt ctaccccatg tgattcttgg gcaggcctcg ctagcggtca
     2521 tcgggaccgt tgacggcatt tcgccacatc gcaagagcac gagcacgccc agcgagaacg
     2581 caaggcagat tccggcgaga aacgacccgc gcgccgatgt catcatgagg gtgaccgacg
     2641 tcgtgaaggg aactcccggc ggtgacacgg tcacgtttcg acccgtaccc accgacgacc
     2701 atgcacgcgc cgcgaaagaa aacgtccctt cattcgccgt gggggaccaa tggctggtct
     2761 tcctcggcag ggacgcgacc gggtacttcg cgatcgattg cacgaacgga ctgtttcgcg
     2821 tgaagggcga ttcgctcatt caagcggaca gggtcgtgct tccggcaacg aagtctacct
     2881 tcatcgcaga aatgcggaag gccgacgcag cccgctaacg catccgatcc ccgccttgat
     2941 gcccgcgccc gcgggcctta tactgcaagc acacccttaa ccgcagtccg tttccgcgct
     3001 ttctaagtct atggccaaca aacgcattct ctgggtcgac gacgagatcg agctcttgaa
     3061 gtcgcacgtg atgttcctgc gcgacaaggg ttacgacgtc caaaccgcca ccaacgggac
     3121 ggacgccgtg aacctggtca aggaggggcg cttcgacatc atgctgctgg acgagtcgat
     3181 gccggggcgc ggcggcttgg agaccttggt cgagatcaag gagtcggacc cggtccttcc
     3241 gatcgtgatg atcacgaaga acgaggaaga gcggctgatg gacaacgcga tcgggatgaa
     3301 gatcgacgac tatctcctga agccggtctc gccgctgcag atctattcgg cctgcaagcg
     3361 catcctggat gcgcccaaga tccaggagaa ccgactgtcg ccggactaca tccgcgagtt
     3421 caacgagatc aattcgctcg cgcaagaagg gacgtgggac gcgtggctgc gcgtgcaccg
     3481 tcgtctgtgc gcgtgggacc gcgagttcga ccagttccgc aacacggggc tggaggcgac
     3541 gcacgacgac cagcgcctgc agacggcgca ccacttcggg ctcttcgtgg agaagaacta
     3601 ccgcgactgg gtcgcctccg acaagcggcc gcccatgagt ctggacgtgt tcccgcgctt
     3661 catcgccccg catttgttga agggcgagaa gctggcgttc atcttggtcg actgcgtccg
     3721 cctcgaccag tggatggcga tcgaggattt cctgtccgag cagttcgacg tgcagtggga
     3781 tttctattgc tcgatccttc ccaccgcgac gccctactcg cgcaactcga tcttcagcgg
     3841 gatgttcccc gacgacatcg cgcgccgcta cccggacaag tggctcgagc gcgtcaacga
     3901 ggactcgagc aagaacaagt tcgaagcctt cttcatgggc gagcagatga agaaactcaa
     3961 gctcgacgag agcaagatgc gctacatgaa aattcatacg gcccaggaag cgggggacgc
     4021 gcgcaagcgc gtggcctcca tgatgggctc gcccttcgtc tcgttggtgt tcaacttcgt
     4081 cgacatcctg acgcacgggc gcaatcaatc ggacattttg cagcagctct tgcccaacga
     4141 gtccgcgttt cgctcgctca tgcgctcgtg gtactcgcac tcggtcctgc gcgacgttct
     4201 cgcggacttg gcgcgcgcca aggtcaaggt ggtgctcacc accgaccacg ggtcgattct
     4261 gggtcgcaag gcagcactgg tgtacggacg ccgcgacacc tccaccaacc tgcgctacaa
     4321 gttcggcgat aatctgaaat gcgacgagcg ccaggccatc atcacgcgca agccctccga
     4381 gttccgcttg ccgtcggaat ccggaaccaa gacgtacctc tttgccaagg agtacttcta
     4441 cttcgtctac ccgaccaact tccgcgacta cgagaaggcc tacgagggaa gcttccaaca
     4501 cggcggcgtc tctctcgagg aaatgatcct cccctgtttg accctgacgc cgcgctgacg
     4561 cggcgcccgc gaaccatgtc gcgagccatc gcccagacgg tacgcacgaa cgacgcggcc
     4621 gaaaccgagc ggttcggagc ggcgctgggt gcctgcatcc accgcggggc gggcgtgtgc
     4681 ttgacgggtc ccttgggcgc gggtaagacg gtgctggtca ggggactgtg ccgagggctg
     4741 ggggccgtcg aacccgttac gagcccgacc ttcattctga tggaatcgtt cgcggggaga
     4801 cttcccatcg tgcacatcga tttgtaccgc ctcgagcacg agcgcgaggt cgaggacatc
     4861 ggcgtgttcg acatgctcga cggcgagacg gtggtgatcg cggagtgggg cgagcgctcg
     4921 ccggcgctcc tcgaagagtg cgacctcgag attcgcatcg agccggggga aggaacgacg
     4981 cgcacgatcc acgtccacgc cacacccgat ttcgcgcgcg agctcgaggg actctcatgg
     5041 tgacgatcgc catcgatacc tcgcacccgg tgggcacgac cgcgctggcg cgcgacggcg
     5101 tcgtgctggg agaggcccgc ttcgactcgc cctcgtcgca cctggtcgag ttgtcgaagg
     5161 cgatcgagcg cttgctcgca tcgagcaacc tcacgatgcg cgacgtcgat cgtatcgcgg
     5221 tggtgctggg gccggggagc ttcacgggcg tgcgcatcgc ggtctcgttc gcgaaagggc
     5281 tcgcggcggc gggcgcgcag gtggttgcga tggactcgct gcgtttgctg gcgctgccgt
     5341 ttttcgacca aggacgtgcg cgcgtgtgcg ccatgatcga cgccaagcgc ggcgaggtgt
     5401 acggcgcgct cttcgagcgc gcctcgggaa gcgtcgaggc cgtcgtcgcg ccaagcgcgg
     5461 ggtctgcggc caagttgcta gaagcgatcg ctacagcacc ggacgtgttc gccggaacgg
     5521 gcgccctcgc gcaccgcgac gaaatcctgg cccgcttccc gcgcgcggag gtcgccggcg
     5581 aagcacgcgc gaatccttcg acgacgtact tcgcgtcgat tgcgcaccac ctacccgcgc
     5641 tcgcgcgcga cgccgtgcgg gcgctcgagc ccgtctatct gcgcgcgagc ggggccgagc
     5701 gcaagcgtct tcgcgcccac gcgccggaac cgggggcgag cggcgatgac tgaccagccc
     5761 gccttcgtcc tcgtgccgct cgagcatcag catattcccg acgtgctgaa gatcgagcgc
     5821 gaggcctttc ccgcgccctg gaccgaagcc atgttccgcc aggaggtcga ggagaagtgg
     5881 ctctcgcggt ctttcgtggc attgctcggg gaggagattg tgggctatgt cattgcttgg
     5941 ttcctgcgcg gggaagtgca cgtgctgaac ctggccgtca ccaaggcgca ccagcgccag
     6001 ggactcgcgc gcgccatgct gcgccacgtg atcggactcg cgtccgagga ggcctgccac
     6061 ctcatcacgc tcgaggtgcg cgtgtccaac gaccccgcca agctcctgta cctctcgatg
     6121 ggcttcgcgc ccgtcggcat tcgccgccgt tactaccacg acaacgacga ggacgcgttg
     6181 gtcatgacca agcggctgcg cggatgagcg ccgcggccac gcgctcgctc gccttcattg
     6241 ccggcagcgg catggacccg atcgcgatcg cgatgaccgt cgggcgcgcg acgcgcttcg
     6301 accagatcgc gggggtgggc gcgcccgggg tcgaagggca cacgggccgc gtgttggagg
     6361 ggaccatcgg cgggggcgcg tgcacgctcg tgatggggcg acgccacgtc tacgaaggcg
     6421 acccggccgc ggtggcacac ctggtcgcgt ggctcgccga gcgcggcgtc accgacttgg
     6481 tgatggcgtc ggcggccggc gcgctgcacc gaggcctcgc cgcgggcgac ctgatggtcg
     6541 cgcacgatat cgtcgacttc cagaaccgcg accgcctcat gcgcgtggct gggtcggcga
     6601 tgaggacagc gtcggctgag aggcttcgaa tcgaccgcgg cctttcggcc gccattgagc
     6661 gcgcggctac cgccgcgcgc gtctcgtggc aacgcgggat catggtgtgc ggtgtggggc
     6721 ccgcctacga aacccaggcc gaggtgctcg cgttacagga gtggggcgac gcggcgacca
     6781 tgtcgtcggc gccggagctg gccgcggcca gccggctggg ccttcgggcg gccgcggtgg
     6841 cggtgatcac gaacccgtgc acgggcgtcg cgtcctggcg tcccaaccac gccgaggtgc
     6901 tcgacgtggg gcgcaagatg gccgtcggcc tggccgaggt cattcgacaa ctcgttatcg
     6961 attaacgcgt tacgagttct cgtcggcgcc cgcccgcggg cgcccgatta ggagttgtgg
     7021 cctgctgtcg cgggcccgta tagtctcaac acaccccaag cacccaggga aggacggtgc
     7081 ttttcgaatt gtcctggttc cgtagggaaa gacggggcgt ccagcccatc cagaagaagg
     7141 ccatccccga cggcctctgg aagaagtgcg acctgtgcgg ggaaatcctc tacgtcaaag
     7201 agctcgagaa gcggttgtgg acctgcacca agtgcggcaa ccacttcatg atctcgaccc
     7261 agcagtacat cgatatcctg gccgacccgg ggtcgttccg cgagacccac accggcttga
     7321 tctccggcga cccgctccag ttcaaggacc tcaagaagta caaggaccgc atccgcgagt
     7381 accacgagaa gaccggcatg gaggacgcca tggtgaccgg cttcgcgtcc atggacggtc
     7441 gcgacgtcgt gctcgctatc atggacttct cctacatggg cggcagtatg ggctcggtgg
     7501 tgggcgagaa attcgcgcgc gcggcgcaag acgccatgct gcgccggcgc ccgctgatcg
     7561 cgctctcgcg ctcgggcgga gcccgcatgc aggagtcgat cttctcgctc atgcagatgg
     7621 ccaagaccgc cgcgatgctc tcgcgcatgt ccgaagaagg actcccgttc atctcgatcc
     7681 tgaccaaccc gaccaccggc ggcgtcaccg ccagtttcgc cagcctgggc gacgtcatcg
     7741 tcgccgagcc gaaggcgttg atcgggttcg cgggaccgcg cgtcatccag cagaccatcc
     7801 ggcaggacct ccccgaaggg ttccagcgca gcgagttcct gctcgagcac ggcatgatcg
     7861 acatgatcgt gtcgcgcgac gagctgcgcc gcacgctggc gggtgtgctc gacttcatgt
     7921 gcgcccgtgc ggcatccgcc ccggaccagg aagtgaagga tgactcgcag tcgccggccg
     7981 tttcctgatt cccccgtagc actaccgccc aaccttcaat tcgtcctcgc gctctcgcct
     8041 tcggtgatga cgctcgggct cgaccacatg cgggccctga tggagcgcgt cgggcatccg
     8101 gagcgtcgct tccgctccgt gctggtcgcc ggcaccaacg gcaagggctc gaccaccacc
     8161 atgctggccg cgatcctggg cgcgcacggg ctgcgcaccg gtcgctacac ctcgccgcac
     8221 gtgttcaacg tcgccgagcg catcacgatc gacggcgcgc acgcgtccat cgaggagatg
     8281 gaagtagccg cggcgcgcct ggtgccgttg cgtgacgaga tcccgttcag ctatttcgaa
     8341 gccctgaccg cgatcgcgtt catggtcttc gccgcgcgcg gggtcgaggt ggcggtgctg
     8401 gaagtcggcc tgggcggccg cctggacgcg accaacatcg tcgagcccga cgtctccatc
     8461 gtcaccagca tctcgctcga ccaccggcgc attctgggcg acaccgaaga gcaaatcctg
     8521 gtcgagaagc tgggtgtgtc gcgccccggc gtgcccttgc tcgtcggtcc gctcccggac
     8581 gcgctgcgcg ccgtggtgaa cgatcgcgcg aagcgcgatg gctttccggt gtttgacctg
     8641 gagaacattg ggcgcgtgtc gcaggtcgag gaagccttcg acgggatcgc gttccgggtc
     8701 gagacgccgc acgccgagta cgggcgcgtc tcgattccgt tcgccgggtc gcaccaagtc
     8761 acgaacgctc tcctcgccat cggtgccgcg gagcggatgc tccccgccct ggtcggactc
     8821 ggcggcgcgc tcgcgcgcgc ctacataccg gggcgcttcg agcgcgtcga gcgcggtggt
     8881 cgctcgttcg tcatcgacgt cgcgcacaac gaggcctcgc tcgtcgcgac cgccgaccac
     8941 ctggccgcgt ttcgcccgcg cgacgagtgc gcgatcgtgt tcgggctcat gcaccgcaag
     9001 gagttgttcg aggcgccccg gcacctgctc gcggcggcgt cgtgcctgtg cttgatcgag
     9061 ccgcccagcg agcccggcag caccgacaac gccttcgccc cgcacgaatt gtgggcgcgc
     9121 tacttcgctc cgctcttgcc ggattcggcc accaacgtca tgctgtggaa cccgtcgggc
     9181 ccgcgcgccg acgccatgcg ccggctcgtc cgctggctgg accgatcgcg ctattcgacg
     9241 gtggtggcga cggggtcgca tcgcgtggtc gaagacttcg ggctgctatt gcagacatct
     9301 accgtagagg aaggcggcaa tacgtccaaa tgagcgcgtg gatcggcatc gacatcgggg
     9361 gcaccaacat caagatcgcc accatctcgg cgcacggaga ggtgttcgag cgcggggtgg
     9421 tggagacgca gccggccgac gggcccgagc gcgcgttcgc gcgcatccac gccgccgcgc
     9481 gctacctggc cgcacaccgg tcgatccagg gtgtggggat aggctgcgcg ggcctgatcg
     9541 attcccggcg cggcattctc cgcgagtccc ccaacctgcc ggcgtggcgc
//
LOCUS       DASPGK010000058         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_130875, whole genome shotgun sequence.
ACCESSION   DASPGK010000058 DASPGK010000000
VERSION     DASPGK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130875"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1611)
                     /locus_tag="VEC56_01495"
     CDS             complement(64..1611)
                     /locus_tag="VEC56_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXV12857.1"
                     /translation="MTPSSQRLHPVSVVFLVFGTLKGFLLPAAVVVMTARSSTWEIWA
                     LWLSGPMALYAGYRYFTTRYAFADDELVIRTGLVFKNERHIRYARIQNIETVQNPLHR
                     ALRVAEVRVETGGGTEQEARLQVLSLDAVDEMRRRVFEGKRRSIGGDPAATAVPDAVS
                     SPSDDEGATATDALPASVDEAPRVLVRLGLRDLAIFGLVQNRGGLVVAAVLGVLWEAD
                     ILDWDAGNSVNMRRLIRFAIDSGVVSNGNLAVIAVLAGSFVAFLIVVRILSIGWAIVR
                     LYDFTLTRSGDELRTSCGLLTKVRTVIPLRRIQLVTMRQAPLQRAFDRVELRVQTAGG
                     DKNASPSREWLAPMLRRDAVDALMAEVLPGSGFDAMPWRPVDRRAAWREFRSWLRLIA
                     AAALIGMWFNPAITVVAAVVLAVLAFFCARGRVRSFGFALAGDRIGCRGGWLWRNASL
                     ARYAKLQVVNMSESPFDRRWKMASVSADTAGGGAHRISIPYLPVAEARTVFDDLGARV
                     NETSFRW"
     gene            complement(1598..2140)
                     /locus_tag="VEC56_01500"
     CDS             complement(1598..2140)
                     /locus_tag="VEC56_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXV12858.1"
                     /translation="MSDEHAAPSSDAPDPIADGALHSLDPRHVTHGRLVGGITVLAIA
                     TGVLIAMIIVLLAVDGMPKSLRLSIPLATLATIVGLGILAWRWPVLEHRRTAYRVNDD
                     GIEIRKGVYWRQVINVPRSRIQHTDVSQGPLERNFELSTLHVFTAGTEHSEVTLPGLA
                     HPLALRIRDHLLTGDEHDAV"
     gene            complement(2137..>4347)
                     /locus_tag="VEC56_01505"
     CDS             complement(2137..>4347)
                     /locus_tag="VEC56_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV12859.1"
                     /translation="GVVHRDIKSDNIVLTPKGQVKVLDFGLARGRDAAAFVTRIGSSA
                     GTPAYMSPEHIQGAEMDARSDLFSFGVVLYEMLTGRMPFKGEHEAALTYSIVNETPTA
                     IHTHNQAVPRGLENIVAKLLEKNPKDRYQGAVEVIEDLRRLEAGQRVKAHPRRRSRVA
                     AAVVAGVLVPLVAFWLVQQRSQRRDVAATDDAGRKMLAVLPFQNLGPTDQEYFADGIT
                     EEITTQLAKLSGLGVISRTSAMKYKGSNKSLREIGKELGVDYVLEGSVRWDKSGAQNN
                     VRVSTQLIRIADDTHLWADNFDRVYDQIFALQSEIAQKVASELNVTLVEPERAALAAR
                     PTKNLDAYDLYLRGRAQLGDATTTEEMDDALVMVEQAVAMDSTFARAQAFLARTYANQ
                     HFNHLHPELPRVKQARAAALAAVRHAGDQPAGHIAMGYYYYYAERDYDRALGEFEKAA
                     KRQPNDADVLEAIAYVQRRQNKWDASVANLERAIELDPTNANRITNLIQTLGYMRRWT
                     EAEQWATRGREMDPRSLQFPSYLAVAKVARDGDTRGAGELLDPIVSKHGAIATFRADI
                     YIMARDYEAVRKLLPNLRAYPNIDTLQFYVIRATASYFDGDHDTAKVFADSALAYLNG
                     RKAATSSDADLVAGYASVLAMLERREEALRAIDRAVSMMPIEKDALAGADIRNARMIV
                     YIAVGDYEAAIKELEFLMSVPSNLTPAILRMHPGFDPLRDNPRFKKLAEEKVSS"
BASE COUNT          711 a         1412 c         1485 g          739 t
ORIGIN      
        1 tgtccgcctc gcgctgcagt ttgagcggag agggacgatc gcaccggctg gcagggaccg
       61 cggttaccag cgaaatgacg tctcgttgac gcgcgcgccc aggtcgtcga agaccgtgcg
      121 cgcttcggcg acagggaggt aggggatcga gatgcggtgc gcgccgccgc cggccgtgtc
      181 ggcggagaca ctcgccatct tccagcgccg atcgaagggc gactcgctca tgttcaccac
      241 ttgcagcttc gcgtagcggg cgaggcttgc gttgcgccac aaccaaccgc cgcggcaccc
      301 gatgcggtcg cccgcgagcg cgaacccgaa cgagcgcacg cgcccgcgcg cacagaagaa
      361 agccagcacg gcgagtacaa ccgccgccac caccgtgatg gccgggttga accacatgcc
      421 gatcagtgcg gcggctgcga tcagccgcaa ccaggatctg aactccctcc acgcagcccg
      481 acgatccacg ggccgccacg gcatcgcgtc gaagccagaa cccgggagta cttcggccat
      541 gagggcgtcg accgcgtcgc ggcggagcat gggggccagc cactcgcggc tcgggctggc
      601 gttcttgtcg ccgccggctg tctgcacgcg cagttcgacg cggtcgaacg cgcgctgcag
      661 cggagcctgg cgcatggtga cgagctggat gcgccgcagc gggatcacgg tccggacctt
      721 ggtgagcagg ccgcacgacg tccgcagctc gtcgccggat cgcgtcagcg tgaagtcgta
      781 gagtcgaacg atcgcccaac cgatcgatag gatccgcacc acgatcaaaa acgcgacgaa
      841 acttccggcg agaacggcga tcaccgcgag attgccgttc gagacgacgc ccgaatcgat
      901 ggcgaatcgg atcaggcgtc gcatgttgac ggaattgccg gcgtcccaat cgaggatgtc
      961 ggcttcccac aggacgccga gcacggcggc gaccaccagt ccgccgcgat tttgcaccag
     1021 cccgaagatg gccaggtcgc gcaggccgag tcgaaccagg acgcggggcg cctcgtcgac
     1081 ggaagcgggc aacgcgtcgg tcgcggtcgc cccttcgtcg tcgctcggac tggacacggc
     1141 gtcgggcacg gcggtggccg ccgggtcgcc gccgatgctt cgccgcttgc cttcgaacac
     1201 ccggcgccgc atctcgtcga ccgcgtcgag tgagagcacc tgcaagcgcg cctcctgctc
     1261 ggtgccgccc cccgtctcca cccgaacttc cgccacccgc agcgcgcggt gcagcggatt
     1321 ctggaccgtc tcgatgttct ggatgcgcgc gtagcgaatg tggcgttcgt tcttgaacac
     1381 caggccggta cgaatcacga gctcgtcgtc ggcgaatgcg tagcgcgtgg tgaagtaacg
     1441 gtacccggcg tacaacgcca tcgggcccga gagccacagc gcccagatct cccacgtgct
     1501 gctgcgcgcc gtcatcacga cgacggcggc cgggagcaag aatcccttca gcgtcccgaa
     1561 aaccaggaag acgacggata ccggatggag gcgctggcta gacggcgtca tgctcgtcac
     1621 ccgtgagcag atggtcgcga atccgcagcg cgagcggatg cgcgagtccc ggcagcgtca
     1681 cttccgagtg ctcggtgccc gcggtgaaca cgtgcagcgt ggacagctcg aaattgcgct
     1741 ccaggggacc ttgcgacacg tcggtatgct ggatgcggga gcggggcacg ttgatcacct
     1801 ggcgccagta cacgcccttg cggatctcga tgccgtcgtc gttcacgcgg taagcggtgc
     1861 gacggtgttc gagcacgggc catcgccacg cgagaattcc cagcccgacg atcgtcgcca
     1921 gggtcgcgag aggtatcgat agccggaggg acttcggcat gccgtcgacc gccagcagca
     1981 cgatgatcat cgcgatcaag accccggtcg cgatggcgag cacggtgatt ccgcccacca
     2041 ggcggccgtg ggtgacgtga cgcgggtcga gggagtggag cgcgccgtcg gcgatggggt
     2101 cgggcgcatc ggatgagggt gcggcgtgct cgtcgctcac gagctgacct tttcctccgc
     2161 gagcttcttg aagcggggat tgtcgcggag cggatcgaag cccgggtgca tgcgcaggat
     2221 cgcgggtgtg aggttggacg gcaccgacat caggaactcc aactctttga tagctgcctc
     2281 gtagtcgccc acggcgatgt agacgatcat gcgcgcgttg cgaatgtcgg cgcccgcgag
     2341 cgcatccttt tcgatgggca tcatcgagac cgccctgtcg atggcgcgca gggcttcctc
     2401 gcggcgctcg agcatcgcga gtaccgacgc gtatccggct accaggtccg cgtcggacga
     2461 agtcgcggcc ttcctgccgt tcagatacgc gagcgcggag tcggcgaaga ccttggccgt
     2521 gtcgtggtcg ccgtcgaaat acgacgcggt ggcacggatg acgtagaact gcagcgtgtc
     2581 gatgttggga tacgcgcgca ggttgggaag cagcttgcgc acggcttcgt agtcgcgcgc
     2641 catgatgtag atgtcggcgc gaaacgtcgc gatcgccccg tgcttgctca cgatgggatc
     2701 gagcagctct ccggccccgc gcgtgtcgcc gtctctcgca actttcgcga ccgcgaggta
     2761 gctcggaaac tgcagcgagc gggggtccat ctcccggccg cgcgtggccc attgctccgc
     2821 ttcggtccag cgccgcatat acccgagcgt ctggatgaga ttcgtgatgc ggttcgcatt
     2881 ggtggggtcg agctcgatgg cgcgctcgag attcgccacg gaagcgtccc acttgttctg
     2941 ccggcgctgc acgtaggcga tggcttccag tacgtcggcg tcgttgggtt gccgcttcgc
     3001 cgctttttca aactcgccca gcgcgcgatc atagtcgcgt tcggcgtagt agtagtagta
     3061 acccatggca atatggccgg ccggctggtc gccggcgtgg cgcacggccg ccagtgccgc
     3121 ggcgcgcgcc tgcttgacgc gcggtagttc ggggtgcaag tggttgaagt gctggttcgc
     3181 atacgtgcgg gccaagaacg cttgcgcgcg cgcaaaggtc gagtccatcg ccaccgcttg
     3241 ctcgaccatc acgagcgcgt catccatctc ctcggtcgta gtggcatccc ccaactgcgc
     3301 gcgtccgcgc aagtagaggt cgtaagcgtc caggttcttc gtgggccggg cggcgagtgc
     3361 ggcccgttcg ggctccacca gcgttacgtt cagctcgctc gccactttct gggcgatctc
     3421 gctttggagc gcgaagatct ggtcgtacac gcggtcgaaa ttgtcggccc acaggtgggt
     3481 gtcgtccgca atacgaatga gttgggtact gacgcggacg ttgttctgcg cgcccgactt
     3541 gtcccagcgc accgagcctt ccagcacgta gtcgacgccg agctccttgc cgatctcgcg
     3601 cagcgacttg ttcgagcctt tgtacttcat ggcgctggtg cgcgagatga cccccagccc
     3661 ggacagcttg gccagctggg tggtgatctc ctcggtgatg ccgtcggcga agtactcctg
     3721 gtcggtgggg ccgaggtttt ggaaggggag caccgcgagc atcttgcggc cagcgtcgtc
     3781 ggtagcggcg acgtcgcgtc gctgcgagcg ctgttgcacg agccagaacg cgaccagcgg
     3841 caccagcaca cccgccacga cggcggcggc cacgcgcgag cggcggcgcg ggtgggcctt
     3901 gacgcgctgg cccgcctcca agcgtcgcag gtcctcgatc acttccaccg cgccttggta
     3961 gcggtccttg gggttcttct ccaggagctt ggcgacgatg ttctccagcc cgcgcggaac
     4021 cgcctggttg tgggtgtgaa tcgccgtcgg cgtctcgttg acgatcgaat aggtaagagc
     4081 cgcttcgtgc tcgcccttga acggcatgcg gccggtgagc atctcgtaca gcacgacacc
     4141 gaacgaaaag agatccgagc gggcgtccat ttctgcgccc tggatatgct cgggcgacat
     4201 gtacgccggc gtccccgcgg aagagccgat gcgcgtcacg aatgcagccg cgtcgcggcc
     4261 ccgcgccagc ccgaagtcga gcaccttcac ctggcccttg ggtgtcagca cgatgttgtc
     4321 ggacttgatg tcgcggtgga cgacgcc
//
LOCUS       DASPGK010000059         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_482821, whole genome shotgun sequence.
ACCESSION   DASPGK010000059 DASPGK010000000
VERSION     DASPGK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482821"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VEC56_01510"
     CDS             <1..485
                     /locus_tag="VEC56_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12860.1"
                     /translation="PLDSVAIVRVAPPAPEEITVYATEFGSGVGSFIDRNVIGGRSYR
                     YRLTASTTTGGEYRSGDVSATVPVFANQLAQNAPNPFNPATTIVYSLSQRASVAVSIF
                     DVTGVPIRRIEQGVRDAGEYAVEWDGRDDAGIVVGTGVYFYRLDGVTGVTPKKMVLLK
                     "
     gene            604..2505
                     /locus_tag="VEC56_01515"
     CDS             604..2505
                     /locus_tag="VEC56_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12861.1"
                     /translation="MRTIGAIVLVLCLPAGLRAADPEHLWSQSFQALPVGQAPAFFTV
                     SFVTVDHLGRTTVAGAFAGATDFGGGPLQTDATGDAFVVSFDTLGVHRFSRAFAGQGF
                     SVPSVRSVAVDGTGAVVLVGIPSDGIDFGGGLLSGNALFIAKFDADGVHSWSKGFPGG
                     IAHGVAVDAADDIIVIGDLTNRIDFGGGLLQTQGANDVFLVKFDANGTHVWSRRFGAA
                     DNQLGRLIAVDASGSIRAAGTFRGRLDFGAGALTTVGEDAYVAAFDTEGNHVWSRKLG
                     DESAQPLGALDVDADGNTLIVGNFSGWIDVGSGRWDSPPPDQDGFVASYTANGTHRWS
                     LHVGGSLRQNVAGAAFDARGRAIVGGSFEGSLALGQAVLVSEGAADAFAARIDPTGAI
                     GWIQRFGGPRGDAISNVATDPDGDMVFRGGNADPIDLGGGLVPGGGWFLASFGKRLPP
                     PVLDITLFARGSAIEVRWGVSTEHGVESIAIERTRAPGSTPELVFSGPLGSGQGVYLD
                     RDVVAGTSYRYELILTTTSGHEFRSSVVSASVPVFPNQLSQNAPNPFVQTSIAYSVSA
                     RADVVLVIYDVSGALVRRIDQGSREAGEYVVEWDGRDDAGNPVGSGVYFYRLDGVAGV
                     SPRKMVLVR"
     gene            2602..>3056
                     /locus_tag="VEC56_01520"
     CDS             2602..>3056
                     /locus_tag="VEC56_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12862.1"
                     /translation="MLLRSTILVCAVAVGTAAADPQHHWSSHFSSPRFRVDAIAVGPA
                     GDVVIVGYADAYTDFGNGRVPGSDPDVFAAKFDAFGDLAWARFFPGNYSLQQPRALAI
                     DSRGNIVMVGYYTATIDFGGGPMTANNLDVFVAKLDSDGGHRWSRHFSKV"
BASE COUNT          468 a          970 c         1048 g          570 t
ORIGIN      
        1 agccactcga cagcgtggcc atcgtgcggg tggcaccgcc cgcgccggag gaaatcacgg
       61 tttatgcgac cgagttcggt tcgggagtcg gctcgtttat cgaccggaac gtgatcggcg
      121 ggcggagcta ccgatatcgg ctcaccgcct cgacgactac gggaggcgag tatcgatcgg
      181 gcgacgtttc ggccacggtc ccggtcttcg cgaatcaact cgcgcaaaac gcacctaatc
      241 cattcaatcc cgcgacgacc atcgtctact cgttgagcca acgcgcctcg gtcgcggtgt
      301 cgatcttcga cgtgacgggc gttccgatac gccgcatcga acagggcgtg cgcgacgccg
      361 gtgaatacgc cgtcgaatgg gacgggcgag acgacgccgg catcgtggtg ggtaccggcg
      421 tctacttcta ccgcctcgac ggcgtcaccg gcgtcacgcc gaagaagatg gtgctgctga
      481 aatagccgac ggcgcgggtg gtcgcttgct ccgcaccgcc gcttgcccta ccatagactc
      541 gtatcccgaa gccgtcgcgc ggccccgcgg cccgcgacgc ccatcttgtt cgggacgcga
      601 gttatgcgga ccatcggtgc cattgtgctc gtgttgtgcc ttccggcggg gctgcgcgcc
      661 gccgaccccg aacatctctg gagtcagagt tttcaggccc tccctgtcgg ccaagccccg
      721 gcgtttttca cggtcagttt cgtcaccgtc gatcatcttg ggcgcaccac ggtggcgggt
      781 gcgttcgccg gcgctaccga cttcggcggc ggcccgctgc aaaccgacgc gaccggagat
      841 gcattcgtcg tttcgttcga cactctcggg gtccaccgct ttagccgcgc gttcgcgggg
      901 cagggtttct cagttccgtc ggtccgatcg gtcgcggtcg atggcaccgg cgccgtcgta
      961 ttggtcggca tcccttcgga tggtatcgac ttcggtggcg ggctgctttc cgggaacgcg
     1021 ctgttcatcg cgaagttcga cgcggacggc gtccattcgt ggagcaaggg gttccccgga
     1081 ggcattgcgc acggcgtcgc ggtcgacgcg gcggacgaca tcatcgtgat cggcgatctg
     1141 acgaatcgca tcgacttcgg cggcggactc ttgcagacgc agggcgccaa cgacgtcttc
     1201 ctcgtgaagt tcgacgcgaa cggtacgcac gtgtggagcc gccgtttcgg agcggccgac
     1261 aaccaactgg gacgactgat cgccgtcgac gcatcgggga gcatccgcgc ggcggggacg
     1321 tttcgtggcc ggctggattt cggcgctggc gcgctgacga cggtgggcga ggacgcctac
     1381 gtcgccgcct tcgataccga agggaaccac gtgtggagcc ggaagctggg cgacgagagt
     1441 gcgcagccgc tcggcgccct agacgtcgac gcggatggaa acacgctgat cgtcgggaac
     1501 ttcagcggct ggatcgacgt gggcagcggc cggtgggaca gcccgcctcc cgaccaggat
     1561 ggattcgtcg cgtcgtacac cgcgaacggt acccatcgat ggagcctgca cgtgggcggt
     1621 tcgctgcgcc agaacgtcgc gggtgcggca ttcgatgctc gcgggcgcgc gatcgtcggt
     1681 ggatccttcg agggctcgct cgcgctcggc caagcagttc tggtgagcga aggtgccgcc
     1741 gacgcgttcg cggcgcgcat cgatccaacc ggtgcgattg ggtggataca acgtttcggt
     1801 ggcccgcgcg gagacgccat ttcgaacgtt gccaccgatc cggacgggga catggtgttt
     1861 cgcggcggca acgcggaccc gatcgacctc gggggcggcc tggttccggg tggtggttgg
     1921 ttcctggcgt ccttcggcaa gcgcctgcct ccgccggtac tcgatatcac gctgttcgcg
     1981 cgcgggagcg cgatcgaggt tcgctgggga gtgagcaccg agcacggtgt ggagtcgatc
     2041 gcgatcgaac gcacgcgcgc cccgggctcg acacctgagc tcgtgttcag cggaccgttg
     2101 ggctcgggcc aaggcgtcta tctcgaccgc gacgtggtgg ccgggacgag ctatcgctac
     2161 gagttgattc tgaccacgac gtcggggcat gagttccgct cgagcgtggt ctcggccagc
     2221 gtgcccgttt tcccgaatca gctctcccag aacgcaccca atcccttcgt ccagacctcg
     2281 atcgcgtact ccgtgagcgc gcgggccgac gtggtactcg tgatctacga cgtctcggga
     2341 gccttggtgc gccgtatcga ccaagggtcg cgggaggcgg gcgagtacgt tgtcgaatgg
     2401 gacggtcgcg acgacgcggg caacccggtg ggcagcggcg tgtacttcta tcgactggac
     2461 ggcgtggcgg gggtgtcgcc gcggaagatg gtgcttgtca ggtaagttcg aatcgcggac
     2521 gcttcttcct ttgaagggcg cgaggaccga atggcgcggc gcttgcactg cgacccttcg
     2581 gcagggtttg gagtcgtccg catgctcttg cgatccacaa ttctcgtctg cgcggtcgcg
     2641 gtcggtaccg ccgccgccga tccccagcac cactggagca gtcacttctc ctcgccgcga
     2701 tttcgcgtcg acgcgatcgc ggtcggcccg gcgggagacg tcgttatcgt cggctatgcg
     2761 gacgcctaca cggacttcgg aaacggacgc gtccccgggt ccgaccccga cgtgttcgcg
     2821 gccaagttcg acgcgtttgg cgaccttgcg tgggcacggt tcttcccggg gaactactcc
     2881 cttcaacagc cgcgcgcgct tgccatagat tcgcgcggta acatcgtcat ggtcgggtat
     2941 tacaccgcaa cgatcgactt cggcggtggg cccatgacgg cgaacaatct cgacgtcttc
     3001 gttgcgaaac tggactccga cggcggccat cgttggagca ggcacttcag caaagt
//
LOCUS       DASPGK010000060        15699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_834577, whole genome shotgun sequence.
ACCESSION   DASPGK010000060 DASPGK010000000
VERSION     DASPGK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 15699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15699
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834577"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VEC56_01525"
     CDS             complement(<1..627)
                     /locus_tag="VEC56_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXV12863.1"
                     /translation="MIEYIPGLEKVPAAESSISYIDGEKGILEYRGIRIEDLAAHSTF
                     EETAYLLLTGKLPTEQELSDLTQRLARARSLPKDLQNVIKAVPKSAHPMAALQTCVAA
                     VGMVSPHQNIYDEKSREASAVDFIGKFPSLVTAIHRVRNNQEIIPPDPSLPIAADFLH
                     MLSGDKPDMHKARAMDICLILHADHTMNASTFTTRVTASTECQPAAALS"
     gene            complement(735..1679)
                     /locus_tag="VEC56_01530"
     CDS             complement(735..1679)
                     /locus_tag="VEC56_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXV12864.1"
                     /translation="MAEALKNTFQWSVSRDRTFRECPRQYWFNHYGFWGGWEFGVDDR
                     TREIYVLKQLKSRATWVGEVVHDCIKHSVENLSRGIPVLPVERILEITRNRMRNDYRV
                     SRDGLYRDNPKHACGFFEHEYRVPVTPEEWRDSADAVDRCLVTFYASEVWDQLKRTRP
                     GDFLEIERFSNFLLDGVGVTVKLDCATRETDHVVVWDWKTGRREGADAPLQLACYAFY
                     IHQAYGIPIHRVKMRRFELSGGTVHERTIAEKELTEILGYVRGSIADMQSLLEDRERN
                     LAREDRFSKVERREACLRCNFLKVCAPSIGPGILNPRA"
     gene            1715..2371
                     /locus_tag="VEC56_01535"
     CDS             1715..2371
                     /locus_tag="VEC56_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV12865.1"
                     /translation="MFRIESLVVGPFQSNCHVVACSETNQAVIFDAGDDATRILALVR
                     RMGVDVKAVINTHAHIDHVSGLADVVAALGVPVFMNQDEAPMYEGVGQQAMMFGLTPP
                     RAVPIHRWLSEGDVINVGKLRGDVMLMPGHSPGHIIVVFAGETPPRAIVGDVLFQGSI
                     GRTDLPGGDHDVMMQTLERFLPMGDDTIVYPGHGPETTIGAEKRWNPFLAPLAQRARR
                     "
     gene            complement(2375..2917)
                     /locus_tag="VEC56_01540"
     CDS             complement(2375..2917)
                     /locus_tag="VEC56_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12866.1"
                     /translation="MKRIAIAAVVCLSVLPAHADHIGIYPEPLATVCVGPPPLPFSNA
                     SVYVVHQFSTGSTGSGWSVGVDLNGLVYLGSACISNPLIGDPINGAHFSYGGCLSAPI
                     TVCQLTFLYANPQPVAGCYHLQVEPYPGDAAVQVFDCADNAHDATGGYFSFELPVILC
                     DDCAVAVEPTTWGAVKALYR"
     gene            complement(2983..4440)
                     /locus_tag="VEC56_01545"
     CDS             complement(2983..4440)
                     /locus_tag="VEC56_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12867.1"
                     /translation="MATTTRTLSLRGLTSFGDVAAEDDAVLDYFLTTDAVTRIKNGEV
                     FLVLGRKGAGKTALVRHFAEGNGRGLSRRLNLAGYPWAVHAQRIDRGSSDVEAYVSSW
                     RYLIAVQLAALALSRTKGSTDESAKAIRQFLTDNYGGESLDLDAVLKPSKLRLSSMSF
                     EPTVLGCKLVGIALDRNAGDVKFGTELNALSDMLLAATVKLARKMGAASLMLHFDELD
                     QGISTLTEERARMLSGLVLAVRAVRQGFREGGVPLKPVMYLRSDLWDKLSFSDKNKIT
                     EKMSLRLEWDSESLLELVTTRLRAKLGAGARWETITQNEQLKAANNKWNFILSRTFLR
                     PRDVIKFMNSALVQAKKRPDEPLVITNPDVVNAREQYSNYLKAELDDEIMAHWPNWEE
                     ALQACSGISTMTFQRDEFVKQYDQRRSGNNPLDADEALEMLYRFSVIGYERRSGYGGS
                     SWAFQYTNPEAGWDSRAPRFKVHLGLKEFAKLRED"
     gene            complement(4567..5469)
                     /gene="lipA"
                     /locus_tag="VEC56_01550"
     CDS             complement(4567..5469)
                     /gene="lipA"
                     /locus_tag="VEC56_01550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXV12868.1"
                     /translation="MSIDQKQRPQRPEWFKIRLQTNQEYKLVNHLVTSLNLHTVCQEA
                     RCPNIYECWGQGTATFMILGDVCTRHCGFCSVMKGKPGPVDPLEPDHVAQAIETLKLK
                     YAVITSVDRDDLPDEGAGHFARVIEAIRRRTPECMVEVLIPDFNGRRELLDVVLDARP
                     VCLAHNIETASHLYRRVRPVAVYENTLKILENSVLYREQHGVDLKVKCGIMVGLGETM
                     EQLLDTLRDIALTGCDILTVGQYLPPKKAALPLDRYYTLEEFAHIKAEGMKMGFKHVE
                     SGPLVRSSYHAKQQVDTLQAPACK"
     gene            complement(5459..6142)
                     /gene="lipB"
                     /locus_tag="VEC56_01555"
     CDS             complement(5459..6142)
                     /gene="lipB"
                     /locus_tag="VEC56_01555"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXV12869.1"
                     /translation="MGSMADRHACRAIDLGVVDYDEAFALQKAHVERLQRNEGDDVLI
                     FVEHPHVITVGRNATGRAIVADRAFIEARGAKVVPTDRGGDVTYHGPGQLVGYPIVRL
                     EPGRRDIRRYVHDLEQVLIDALADFGVEAARHPVHRGVWVREKKIASLGIRISRWVTS
                     HGFALNVSTDLSYFGLMNPCGIEGCTMTSLERECASPVSMPVVRERVTAHFGNVFARE
                     MHEEAAGVH"
     gene            complement(6144..7451)
                     /locus_tag="VEC56_01560"
     CDS             complement(6144..7451)
                     /locus_tag="VEC56_01560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXV12870.1"
                     /translation="MAVDVIMPQMGESIAEGTIVKWLKGIGENVDRDEPLFEISTDKV
                     DAEIPSPSAGVLLEILVPEGKTVEINTVVGRIGAAGEAKQPAERPAPSAPKQAAAPPK
                     PAPAPTPTPAPTAAPTAPPSFVRSSPVVQKIAAEHGVDVSLVEGTGEGGRVTKKDIMD
                     YIASAGQSAPPITAPTPTARPAPTPTTPTQPVAAPKPAAPKPSVFASGQEEVREPMSV
                     MRKKIAEHMVVSKRTSPHVYTIFEVDMHEVVKRRDASKREFEAEGVKLTFLPYFIEAC
                     IRGIKEYPIINSSVDGDTVIYKKTVNVGIAVALENGLIVPVIKHADQMNLLGLAKTAA
                     DLGERARTKRLVPDDVQGGTFTITNVGVFGGTFGLPIINQPQAAIMGIGTITKRPVVI
                     DDAIAIRPIVYLSLSYDHRIIDGADAARFLTVVKRELEKPAGA"
     gene            complement(7482..8456)
                     /locus_tag="VEC56_01565"
     CDS             complement(7482..8456)
                     /locus_tag="VEC56_01565"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXV12871.1"
                     /translation="MAVTTYIDAIKEGIWEEMEKDERVFILGEDVGIYGGAFKVTKGM
                     LDKFGPMRVIDTPISESAIVGAAIGAALVGMRPVAEMQFIDFISCAFDQIVNFAAKCR
                     YRWGAGVPIVVRGPSGGGVHGGPFHSQNPEAYFFNVPGLKIVCPSTAYDAKGLIKAAI
                     RDPDPVLYFEHKFLYRRIKEDLPTEDFTVPIGKAALRREGEDLSIITYGAMVYKALEA
                     AEALAAMGIDVEVLDLRSILPLDEEAILATAKKTSKVIVLHEATLTGGPGGEVVARIA
                     EKAFEYLDGPIVRIAPPDTPVPYSPPLEEAFLPQTKDIVEKAKKLAAY"
     gene            complement(8463..9476)
                     /locus_tag="VEC56_01570"
     CDS             complement(8463..9476)
                     /locus_tag="VEC56_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXV12872.1"
                     /translation="MVERQAMSAKKSRYVHVPDDETKIRMLHFMKLVREIEDRIEKKL
                     YRQGRIVGGVYVGRGQEAISVGTAIHMEKDDVVAPSHRDMGVFLMRGVTPRRIIAQYM
                     GRRTGLTRGRDGNMHMGDMNYNVIAFVSHLGDNVPVAAGVALAFKQRGQKQVALCYNG
                     EGATSRGDWHEGINFASIHKLPVAYFINNNAYAYSTPMELQMAVPDVAIRAAGYGIPG
                     EVVDGNDILAVYDSAKRAIDLARSGGGPSLVEYKTFRMTGHSAHDDAGYVPPELFDEW
                     KRKDPIVRFEKALLDEGVVTQARIDEMQKECALIVDDAVEWAEAQPYPAPEEVTKDVY
                     YEG"
     gene            complement(9469..10482)
                     /locus_tag="VEC56_01575"
     CDS             complement(9469..10482)
                     /locus_tag="VEC56_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXV12873.1"
                     /translation="MVKPSTSDAYSIAVPQGLELDRTGRLELYYFMALNRKLEERLGL
                     LYRQNKVVGGLYSSLGQEACSVGTAYALGHGDFLAPMIRNLGSLLVRGVRPTDVMAQY
                     CARAIGPTRGKDLNVHFGWVDRGLIAPISVVGALIPVMAGIALEMKMHGKKNVVLTYI
                     GDGGMSTTDFHEGMNFAGVHKLPMILVVENNLWAYSTHVSKQANVKDLAVRAEGYGCY
                     GEIVDGNNVLAVLDATRRARARCLSGGGPVMLEAKTFRRKGHAEHDDAGYVPAEQRAE
                     WEKKDPIDAYRRFLVERRVVDSPQEIDEIDAKIVAILEAAANEALSAPFPDPSIATEN
                     VYG"
     gene            complement(10460..11848)
                     /gene="lpdA"
                     /locus_tag="VEC56_01580"
     CDS             complement(10460..11848)
                     /gene="lpdA"
                     /locus_tag="VEC56_01580"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXV12874.1"
                     /translation="MKEFDVAIIGSGPGGYVAAIRAGINGLSTALVEKDPFLGGTCLH
                     RGCIPTKALLHTAELLDEIRHAKDHGIIVPVPLLDIVQVHARKQSVVDKLAKGVAFLC
                     KKRKVEVFTGLGSFVDANTIRITAPADTTIRARNVVIATGSTPSHLPHMKPDGKSIID
                     SDMILQAGPVPKSLAVIGSGAVGTEFASVFQSFGSQVHLIELLPRLLPIEDDECSQQL
                     ERSLKARGIQCYTATEVTEARVGGGGVALTLKKDGAASTLEVEKVLVATGRRPVLDGL
                     GHDKIGVAMNGRFVAIDEYMRTNVPGVYAIGDVVATPALAHVASHEGILAVDHIAGKP
                     VHPIDYLAIPNCTYSHPEVASVGLTERAAKEKGFDIRVGKFPFSAISKAAILGDTAGF
                     VKIVADKRYDEVLGVHIIGPRATELIAEAVLGKRLETTVEAISHTVHAHPTLSESMME
                     AAHAVYGETIHI"
     gene            complement(12064..12432)
                     /locus_tag="VEC56_01585"
     CDS             complement(12064..12432)
                     /locus_tag="VEC56_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXV12875.1"
                     /translation="MNVAVIGASRDREKFGNKAVRAYHAHGHRVFPVNLHEKEIEGLP
                     AFRSVLQIPEPLDVVLVYVPPSVTLRLLPDLAKLRPKQLFLNPGSEDDAVVARAAELG
                     LSPMLACSIVAIGDSPANYR"
     gene            complement(12429..13139)
                     /locus_tag="VEC56_01590"
     CDS             complement(12429..13139)
                     /locus_tag="VEC56_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12876.1"
                     /translation="MRKRTAWSSLTWRLRSGAFGFYVEGGRRLAGTVRLSARPAYRLR
                     PRGVAAIGTGLVLVLLPIALLLTDGSRSIRLAGELVDGRPAEEVASLRGPLERYYKDR
                     EEGRTGREVAVTVSVSAYTSRECETDDTPFITAANTSTRPGVLALSRDLLKRYTPGAP
                     FDFGDIVHINGLGDFVVEDSMHGRWQHRADIWFESVDDAREFGRRPAVLTGPYGLASG
                     QKLTRSIDVASARPGASR"
     gene            complement(13414..14178)
                     /locus_tag="VEC56_01595"
     CDS             complement(13414..14178)
                     /locus_tag="VEC56_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12877.1"
                     /translation="MGNFATARLSALILVLSWTGAQAAETPLPPRAGYTFYVQGQRVG
                     RCDVRITQTDTALRFESTLRVENGSAVIELSTKAEADPRTYALRSFSYEGTKGGMPAT
                     ASVTVAGDSVFGHFSLNGPRKAQRRSVNPRPVVVWEDWAMEIEILLALQQAREFKNPS
                     TRALLLAGSFTSTVVTLGFTGEAVVESADRSMTARKLVVGIEGGDPFESLIDPKLGVP
                     VYIHFPGIRAEAFLDDFFGDNPSPRYSPATDTSSGR"
     gene            14380..15165
                     /locus_tag="VEC56_01600"
     CDS             14380..15165
                     /locus_tag="VEC56_01600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV12878.1"
                     /translation="MSRFPVLMYHRVRSSRTPVADPAERPWAVSLEAFEWQLDRLKAA
                     GKIAVTMAQAHAALGTGSGVPPEWVVLTFDDGNQSDHTHALPLLTARGFRATFFVCGC
                     RVGEAGGLEPEMIREMHAAGMQVGSHAMTHRFLTTLSARDEEDELARSKDLLQGIVDT
                     PVDHFAPPGGRWSKRTAQALRRLGYRAVSTSAFGYNDAREAKFAYRRIPVVDATTRAR
                     FDDILAGERWKLAAGYLRAGSLGLARRAMGEATYARLRGYQDR"
     gene            15176..>15699
                     /locus_tag="VEC56_01605"
     CDS             15176..>15699
                     /locus_tag="VEC56_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV12879.1"
                     /translation="MKLVFFISIATIVYAYLLYPAIIVVWARLFPRRVAKRYRQVPVS
                     VVIAARDEEANISARIENLIAQEYPRALLEVVVVSDGSTDRTAELARHFANHGVNVVE
                     CSSPVGKAAALNIGVATASHGIVVFADARQRFSSNAIAELVSMFHDETVGAVSGELIL
                     LSGDDVGEVHEGVGL"
BASE COUNT         2565 a         5016 c         5295 g         2823 t
ORIGIN      
        1 cgagagggcg gcggcgggct ggcactcggt ggacgccgtg acgcgcgtcg tgaacgtgga
       61 cgcgttcatc gtgtggtcgg cgtgcaggat caggcaaatg tccatcgcgc gcgccttgtg
      121 catgtcgggt ttgtcgcccg agagcatgtg caggaagtcg gcagcgatgg ggagcgacgg
      181 atcgggcggg atgatctcct ggttgttgcg cacgcggtgg attgccgtca ccagagacgg
      241 gaatttgccg atgaagtcca ccgcactggc ctcgcgtgac ttctcgtcgt agatgttctg
      301 gtgcggcgat accatgccga ccgcggcgac acaggtctgc aacgccgcca tcgggtgtgc
      361 gctcttgggt accgccttga tgacgttctg caggtccttg gggagactgc gggcccgggc
      421 gaggcgctgc gtcagatccg agagctcctg ctcggtcggc agcttgccgg tcagaagcag
      481 gtacgcggtc tcctcgaagg tgctgtgcgc ggcgagatcc tcgatccgga tgccccggta
      541 ctccaggatg cccttctcgc cgtcgatgta acttatggaa gattcagcgg ccgggacctt
      601 ttccaggccg gggatgtact cgatcatctg gaatgctcct tcaatcgacg agtgctaagg
      661 cacttatact atagaagccg gtgcggcaca aggccggttt gggcctttcc ggggcctccc
      721 ggaagccccg cggactaagc gcgtggattg aggattccgg gtccgatact gggcgcacac
      781 accttcagga aattacagcg caagcaagcc tcgcgccgct ccacctttga aaagcgatct
      841 tcgcgggcca aattgcgctc gcgatcctcg agcaggcttt gcatgtcggc gatgctccca
      901 cgcacgtagc ccaggatctc cgtcagctcc ttctcggcga tcgtccgctc gtgcacggtg
      961 ccgcccgaga gctcgaagcg ccgcatcttc acgcggtgga tggggattcc gtacgcctgg
     1021 tggatataga aggcgtagca cgcgagttgg agcggggcgt cggcgccctc gcgccggccc
     1081 gtcttccagt cccacaccac cacgtggtcg gtttcgcgcg tggcgcagtc gagcttgacc
     1141 gtgacgccca cgccgtccaa gaggaagttc gaaaagcgct cgatttcgag gaagtcgccg
     1201 ggacgcgtgc gcttgagctg gtcccacacc tcggaggcgt agaaggtgac caggcagcgg
     1261 tcgaccgcgt cggccgagtc gcgccattct tccggggtga cgggcacgcg gtactcgtgc
     1321 tcgaagaacc cgcacgcgtg cttggggtta tcgcggtaga ggccgtcgcg cgagacgcgg
     1381 tagtcgtttc gcatgcggtt gcgcgtgatc tcgaggatgc gctcgaccgg aagcacggga
     1441 ataccgcgcg acaggttttc gaccgaatgc ttgatgcagt cgtgcaccac ttcccccacc
     1501 cacgtggcgc gcgacttgag ctgcttcaac acgtagatct cacgcgtgcg atcgtcgacg
     1561 ccgaattccc agccgcccca gaaaccgtag tggttgaacc agtattggcg cgggcattcg
     1621 cggaaggtgc gatcgcgcga aaccgaccat tggaaggtgt tcttcagggc ctcggccatg
     1681 gcggcaatat aacacgccgt gctaccatgg ccgcatgttc agaatcgaat cgttggtggt
     1741 ggggccgttc cagagcaact gccacgtggt cgcgtgctcg gagaccaacc aggccgtgat
     1801 cttcgacgcc ggcgacgacg cgacgcgcat cttggcactc gtgcgccgca tgggcgtcga
     1861 cgtcaaggcc gtcatcaata cgcacgcgca catcgaccac gtgtcgggcc tggccgacgt
     1921 cgtggcggcc ctcggcgtgc ccgtcttcat gaaccaggac gaagccccca tgtacgaagg
     1981 cgtcgggcag caagccatga tgttcggcct aacgcccccg cgcgcggtcc ccatccaccg
     2041 ctggctgtcc gaaggcgacg tgatcaacgt gggcaagctg cgcggcgacg tgatgctcat
     2101 gccgggccat tcgccgggac acatcatcgt ggtgttcgca ggcgagacac cgccgcgcgc
     2161 gatcgtgggc gacgtgctgt ttcagggcag catcgggcgc accgacttgc cgggcggcga
     2221 ccacgacgtc atgatgcaga cgttggagcg attcctgccc atgggcgacg acaccatcgt
     2281 gtaccccggc cacgggccgg agaccacgat cggcgccgag aagcgttgga atccgttcct
     2341 cgcgccgctc gcgcagcggg cgcgccgctg acgctcaccg atacagcgcc ttcaccgcac
     2401 cccacgtcgt gggctcgacc gcgaccgcgc agtcgtcgca gaggatgacc ggcaattcga
     2461 acgagaagta tccgccggtc gcatcatgcg cgttgtccgc gcagtcgaag acttggaccg
     2521 ccgcgtcgcc ggggtacggt tccacctgca ggtggtagca tccggccacg ggctgcggat
     2581 tcgcatagag gaacgtcagc tggcagaccg tgatcggagc cgagagacac cccccgtacg
     2641 agaagtgggc gccgttgatg ggatcgccga ttagcgggtt cgatatacac gcggatccga
     2701 ggtagaccag tccgttcagg tcgacaccga cactccagcc gctcccggtg ctgcccgtcg
     2761 aaaactggtg tactacgtaa accgaagcgt tcgagaatgg taacggcggt ggtccgacgc
     2821 acacggtcgc caacggctct gggtagatcc cgatgtgatc ggcgtgggcg ggtaggaccg
     2881 agaggcaaac gacggccgcg atagcaatcc ttttcatggg cagcctcctt tgagtctacc
     2941 catgagtcta gtccggttcc gacgacccgg caacgggccc ggtcagtctt cccgcagctt
     3001 cgcgaattct ttgagtccca gatggacctt gaagcgaggg gcgcggctgt cccagccggc
     3061 ctcggggttg gtgtactgga aggcccacga gctgccgccg tagccgctgc ggcgctcgta
     3121 gccgattacg gagaatcgat agagcatttc gagcgcctcg tcggcgtcga gcgggttgtt
     3181 gcccgagcgg cgctggtcgt actgcttgac gaactcgtcg cgctggaagg tcatggtgct
     3241 gatccccgag cacgcttgca gcgcttcctc ccagttcggc cagtgcgcca tgatctcgtc
     3301 gtcgagctcg gccttcaggt agttggagta ctgctcgcgc gcgttgacca cgtccggatt
     3361 ggtgatgacc agcggctcgt cggggcgctt cttggcttgc accaacgccg agttcatgaa
     3421 cttgatcacg tcgcgcggac gcagaaaggt gcgcgacagg atgaagttcc acttgttgtt
     3481 cgcggccttc agttgctcgt tctgggtgat ggtctcccag cgcgcgccgg cgccgagctt
     3541 ggcgcgaagg cgcgtcgtca ccaactcgag cagggactcg ctgtcccatt ccaggcgcag
     3601 cgacatcttc tcggtgatct tgttcttgtc cgagaacgag agcttgtccc acaagtcgga
     3661 gcgcaggtac atgacgggct tcaagggcac gccgccctcg cggaacccct ggcgcacggc
     3721 gcgcacggcc agcaccagcc cggaaagcat gcgcgcgcgc tcttcggtaa gcgtggaaat
     3781 cccctggtcc agttcgtcga agtggagcat gagcgacgcg gctcccatct tgcgcgcgag
     3841 cttgacggtt gccgcgagca gcatgtcgga gagcgcgttc aactcggtgc cgaatttgac
     3901 gtcgcccgcg ttgcgatcga gcgcgatgcc gaccagcttg cacccgagta cggtgggctc
     3961 gaacgacatg ctcgaaaggc ggagcttgct cggtttgagc accgcgtcca ggtcgagcga
     4021 ctcgccgccg tagttgtcgg tcaggaactg gcggatggcc ttggcgcttt cgtcggtgga
     4081 ccccttggtg cgcgagaggg cgagggccgc gagttggacc gcgatcagat agcgccacga
     4141 ggagacgtag gcctcgacgt cggaggaacc acggtcgatt ctctgcgcgt gcacggccca
     4201 tgggtatcca gcgaggttga gccggcgcga gagacccctg ccgttgcctt cggcgaagtg
     4261 acggacgagt gcggtcttgc ccgcgccctt gcgcccgagc acgaggaaca cctcgccgtt
     4321 cttgatgcgc gtcaccgcgt cggtagtcag aaagtagtcg agaacggcgt cgtcctcggc
     4381 ggccacgtcg ccgaaagagg tcaagccgcg cagtgaaagc gtccgcgtcg tcgtagccat
     4441 ttcagtcctc tccttagtaa acaagggctc gactgcaaac ggcgtgcctt gggcgggtcg
     4501 gtccctatgg gcgcgggtcg cggcgcatga gggcgcgtag tggattcggg ggacgggtgg
     4561 gcgtcgctac ttgcaggcgg gagcttggag ggtgtcgacc tgctgcttgg cgtggtagga
     4621 gctgcgcacg agcgggcccg actcgacgtg tttgaagccc atcttcattc cttcggcctt
     4681 gatgtgcgcg aactcttcga gcgtgtagta gcggtcgagg gggagggccg ctttcttggg
     4741 cggcaagtac tgcccgacgg tcaggatatc gcagccggtg agggcgatgt cgcgcaaggt
     4801 atcgagcagc tgctccatgg tttcgcccaa gcccaccatg atgccgcatt tgaccttgag
     4861 atcgacgccg tgctgctcgc gatagagcac cgagttctcg aggatcttga gcgtgttctc
     4921 gtacaccgcg accgggcgca cgcgtcggta caggtgcgac gcggtctcga tgttgtgcgc
     4981 gaggcacacg ggccgcgcgt ccagcacgac gtccaagagc tcgcggcgtc cgttgaagtc
     5041 ggggatcaat acttcgacca tgcactcggg ggtgcggcgg cgaatggctt cgatcacgcg
     5101 cgcgaagtgg cccgcgccct cgtcggggag gtcgtcgcgg tcgaccgacg tgataaccgc
     5161 gtacttgagc ttcagcgtct cgatggcctg cgccacgtgg tcgggctcga gcggatcgac
     5221 cggccccggc ttgcccttca tgacggaaca gaagccgcag tggcgcgtgc acacgtcgcc
     5281 cagaatcatg aaggtggccg tgccctggcc ccagcattcg tagatgttgg gacagcgcgc
     5341 ttcctggcac acggtgtgga gattgaggct cgtcacaaga tgattgacga gcttgtactc
     5401 ctggttggtc tggagacgaa tcttgaacca ctcgggccgc tgcgggcgct gcttctgatc
     5461 aatggacacc ggcggcctcc tcgtgcatct cgcgcgcgaa cacgttcccg aaatgcgcgg
     5521 tcacgcgctc gcgcacgacg ggcatcgaca ccggcgaggc gcactcgcgc tccaaggacg
     5581 tcatcgtgca gccttcgatc ccgcacgggt tcatcaaacc gaagtacgaa agatcggtcg
     5641 acacgttcaa ggcgaagccg tgcgaggtga cccagcgcga gatgcgaatg ccgagcgacg
     5701 cgatcttctt ctcgcgcacc caaaccccgc ggtgcacggg gtgccgcgcg gcctccacgc
     5761 cgaagtcggc cagggcgtcg atcaggactt gctcgaggtc gtggacgtag cggcgaatgt
     5821 cgcgacgccc gggctcgagc cgaacgatgg ggtagccgac cagctggccg ggtccgtggt
     5881 aggtgacgtc gccgccgcgg tcggtgggaa ccaccttcgc gccgcgcgcc tcgatgaagg
     5941 cgcggtccgc gacgatcgcg cgtccggtgg cgttgcgacc caccgtgatg acgtgcgggt
     6001 gctcgacgaa gatcagcacg tcgtcgccct cgttgcgctg gaggcgctcg acgtgcgcct
     6061 tctgcagcgc aaacgcctcg tcatagtcga ccactccgag gtcgatcgcg cgacaggcgt
     6121 ggcggtctgc catcgaaccc atgctacgcc cccgcgggct tctccagctc gcgcttgacc
     6181 acggtcagga agcgcgcggc gtcggcgccg tcgatgatgc ggtggtcgta cgagagcgac
     6241 agatacacga tgggccggat cgcgatggcg tcgtcgatga cgacggggcg cttcgtgatg
     6301 gtcccgatgc ccatgatggc cgcctgcggc tgattgatga tgggcagccc gaaggtcccg
     6361 ccgaacacgc ccacgttggt gatggtgaag gtcccgccct ggacgtcgtc ggggaccagg
     6421 cgcttggtgc gcgcgcgctc gcccaggtcg gcggcggtct tggcgagccc caggaggttc
     6481 atctggtcgg cgtgcttgat caccggcacg atgagcccgt tttccagagc cacggcgatt
     6541 cccacgttga ccgtcttctt atagatgacg gtgtcgccgt ccacgctgct gttgatgatc
     6601 gggtactcct tgatgccgcg gatgcacgct tcgatgaagt agggaagaaa cgtgagcttg
     6661 acgccctcgg cttcgaactc gcgcttgctg gcgtcgcggc gcttgaccac ttcgtgcatg
     6721 tcgacttcga agatggtgta gacgtggggc gaggtgcgct tgctcaccac catatgctcg
     6781 gcgatcttct tgcgcatgac gctcatgggc tcgcgcactt cttcctgtcc cgacgcgaac
     6841 acggacggct tgggggcggc cggcttgggt gccgcaaccg gctgggtggg tgtcgtagga
     6901 gtcggcgccg gtcgcgcagt cggcgtggga gcggtaatcg gtggcgccga ctgtcctgcc
     6961 gacgcgatgt agtccatgat gtctttcttg gtcacgcgac cgccttcgcc ggtgccttcg
     7021 accagcgaga cgtcgacgcc gtgctcggcg gcgatttttt gtaccaccgg ggagctgcgc
     7081 acgaacgacg gtggtgccgt cggcgccgcg gtgggtgcgg gggtcggtgt gggagccgga
     7141 gccggcttcg gaggcgcagc ggcctgtttc ggcgccgaag gcgccggcct ttcggccggc
     7201 tgtttcgctt cgccggccgc accgatgcgt cccaccacgg tgttgatctc caccgtcttg
     7261 ccttccggca ccaggatctc gagcagtact cccgccgacg gcgacggaat ctccgcgtcg
     7321 actttgtcgg tggagatctc gaacagcggc tcgtcgcggt ccacgttttc gccgataccc
     7381 ttcagccact tcacgatcgt cccttcggcg atactctcgc ccatctgggg catgatcaca
     7441 tcgactgcca tacgaaatgt cctttcctta tctcgacccg cctagtaggc ggcgagcttc
     7501 ttggcttttt caacgatatc cttggtctgc gggaggaacg cctcctcgag cggcgggctg
     7561 tagggcaccg gcgtgtcggg gggcgcgatg cggacgatgg gtccgtccaa atactcgaac
     7621 gccttctcgg cgatgcgcgc gaccacttcg ccgccgggac cgccggtcaa ggtcgcttcg
     7681 tgcagcacga tcaccttgct ggtctttttg gcagtggcga ggatggcctc ttcgtcgagc
     7741 ggcaggatgg agcgcaggtc cagcacctcg acgtcgatgc ccatcgcggc gagcgcctcg
     7801 gccgcttcca gcgctttgta caccatggcg ccgtaggtga tgatcgagag gtcttcgccc
     7861 tcgcggcgca gcgccgcctt gccgatcggc accgtgaaat cctcggtggg gaggtcttcc
     7921 ttgatgcggc gatacaggaa cttgtgctcg aagtagagca ccgggtccgg gtcgcgaatc
     7981 gcggccttga tcaatccctt cgcgtcgtac gcggtcgacg ggcacacgat cttcaggccc
     8041 ggaacgttga agaaatacgc ttccggattc tgggagtgga acggaccgcc gtgtacgccg
     8101 ccgcccgacg gcccgcgcac cacgatgggc acgcccgcgc cccagcggta gcggcacttg
     8161 gcggcaaaat tgacaatctg atcgaacgcg cacgagatga agtcgatgaa ctgcatctcg
     8221 gccaccgggc gcatgccgac cagggccgcg ccgatggcgg cgcccacgat ggccgactcg
     8281 gagatgggcg tatcgatgac gcgcatgggg ccgaacttgt cgagcatccc cttggtcacc
     8341 ttgaaggcgc cgccgtagat gcccacgtcc tcacccaaga tgaagacgcg ttcgtccttc
     8401 tccatctctt cccagatgcc ttccttgatg gcgtcgatgt acgtcgtcac tgccatgggt
     8461 tcctatcctt cgtagtagac gtctttggtg acttcttcgg gcgcggggta gggctgcgct
     8521 tcggcccatt cgacggcgtc gtcgacgatc agcgcgcact ccttttgcat ctcgtcgata
     8581 cgcgcctgcg tcaccacccc ttcgtcgaga agtgccttct cgaagcgcac gatcggatcc
     8641 ttgcgcttcc actcgtcgaa taactcgggc ggcacgtagc cggcgtcgtc gtgcgcggag
     8701 tggccggtca tgcggaaggt cttgtactcg accagcgacg gcccgccgcc cgagcgcgcg
     8761 aggtcgatcg cgcgcttggc cgagtcgtac acggccagga tgtcgttgcc gtcgaccact
     8821 tcgcccggga tgccgtagcc cgcggcgcgt atcgcgacgt cggggaccgc catctggagc
     8881 tccatcggcg tcgagtacgc gtaggcgttg ttgttgatga agtaggcgac cgggagcttg
     8941 tgaatcgacg cgaagttgat tccctcgtgc cagtcgccgc gcgaggtggc gccttcgccg
     9001 ttgtagcaca acgccacttg cttctggccg cgctgcttga acgcgagtgc cacccccgcc
     9061 gccaccggaa cgttgtcgcc caagtggctc acgaacgcga tcacgttata gttcatgtcg
     9121 cccatgtgca tgttgccgtc gcgcccgcgc gtaagaccgg tgcggcggcc catgtactgc
     9181 gcgatgatgc ggcgcggggt gactccgcgc atcaggaaca cgcccatgtc gcggtgcgac
     9241 ggtgccacca cgtcgtcctt ctccatgtgg atcgcggtgc cgaccgaaat cgcttcctgc
     9301 ccgcgcccga cgtagacgcc gcccacgatg cggccctggc gatagagctt cttttcgatg
     9361 cggtcctcga tctcgcgcac cagcttcatg aagtgcagca tgcggatctt ggtctcgtcg
     9421 tcggggacgt ggacgtagcg cgacttcttc gccgacatgg cctgtcgttc aaccataaac
     9481 gttctccgtg gcgatggatg gatcggggaa cggggcggac aacgcctcgt tcgcggcggc
     9541 ttccagtata gcaactatct tggcgtcgat ctcgtcgatc tcctggggag aatcgacgac
     9601 gcgccgctcg accaggaaac ggcggtacgc gtcgatgggg tccttctttt cccactcggc
     9661 gcgctgctcg gcgggcacgt agccggcgtc gtcgtgctcg gcgtgaccct tgcgccggaa
     9721 ggtcttcgcc tccagcatca ccggtcctcc gccggagagg cagcgcgcgc gcgcgcgccg
     9781 ggtggcgtcc agcaccgcca gcacgttgtt gccgtcgacg atctcgccgt agcagccgta
     9841 gccctcggcc cgcaccgcga ggtccttcac gttggcctgc ttcgagacgt gcgtcgagta
     9901 ggcccacagg ttgttctcga ccaccaggat catcgggagc ttgtgcacgc ccgcgaagtt
     9961 cattccttcg tggaagtcgg tggtgctcat gccgccgtcg ccgatgtacg tgagcaccac
    10021 gttcttcttg ccgtgcatct tcatctccag cgcgatgccg gccatcaccg ggatgagcgc
    10081 gcccaccacg ctgatgggag cgatcaggcc gcggtcgacc cacccgaagt ggacgttcaa
    10141 gtccttgccg cgcgtcggtc cgatggcgcg cgcgcaatac tgcgccatca cgtcggtcgg
    10201 gcgcacgccg cgcaccaaca gcgaccccag gttgcgaatc atgggcgcca ggaagtcgcc
    10261 gtggccgagc gcgtacgcgg ttcccaccga gcacgcctcc tggcccaagc tggaatagag
    10321 tccgcccacc accttgttct ggcgatacag gagacccagt cgctcctcga gcttgcgatt
    10381 gagcgccatg aaataataga gctcgaggcg accggttcgg tcgagctcga ggccctgcgg
    10441 caccgcgatc gagtatgcgt cagatgtgga tggtttcacc atagacggcg tgtgccgcct
    10501 ccatcatcga ttcggacagg gtgggatgcg cgtggacggt atgagaaatg gcttcgaccg
    10561 tggtctcgag gcgcttgccg agtaccgctt ccgcgatcag ttcggtcgcg cgcggcccga
    10621 tgatgtgcac gcccagcact tcgtcgtagc gcttgtcggc cacgatcttc acgaatccgg
    10681 ccgtgtcgcc caggatcgca gccttgctga tcgcggagaa ggggaacttg ccgacgcgga
    10741 tgtcgaagcc cttctccttg gcggcgcgct cggtcagtcc gacgctcgcg acttcggggt
    10801 gcgagtacgt gcaattgggg atggcgagat agtcgatggg gtggaccggc ttgcccgcga
    10861 tgtggtcgac ggccaggatg ccttcgtgcg acgcgacgtg cgcgagcgcg ggggtcgcga
    10921 ccacgtcgcc gatcgcgtac acgcccggga cgttggtgcg catgtactcg tcgatcgcga
    10981 cgaagcgccc gttcatggcc accccgatct tgtcgtgtcc gaggccgtcg agcaccgggc
    11041 gcctcccggt ggcgaccagc actttctcca cctcgagcgt cgaggcggcg ccgtcctttt
    11101 tcaaggtcag cgcgacaccg ccgccgccca ctctcgcttc ggtgacttcg gtggccgtgt
    11161 agcactggat gccgcgcgcc ttgagcgagc gctcgagctg ctgtgaacac tcgtcgtctt
    11221 cgatgggcag gaggcgcggg agcaattcga tcagatgcac ctgcgagccg aacgactgga
    11281 acaccgacgc gaattcggtg ccgaccgcac ccgacccgat gacggcgagg ctcttgggaa
    11341 ccgggcccgc ctgcaggatc atgtcgctgt cgatgatcga cttgccgtcg ggcttcatgt
    11401 gcggcaaatg cgacggcgtc gagccggtcg cgatgacgac gttgcgcgcg cgaatggtgg
    11461 tatcagccgg agcggtgatg cggatcgtgt tcgcatcgac gaagctgccc aggccggtga
    11521 acacctcgac cttgcgcttc ttgcacaaga acgcgacccc cttggcgagc ttgtcgacca
    11581 cggactgctt gcgcgcgtgc acctggacga tgtcgagcag gggaaccggc acgatgatgc
    11641 cgtgatcctt ggcgtggcgg atctcgtcga gcagctcggc ggtgtggagc agcgccttgg
    11701 tcggaatgca cccgcgatgc aggcaggtcc cgccgaggaa ggggtccttc tcgaccaggg
    11761 ccgtcgaaag gccgttgatg ccggcgcgga tcgcggccac ataaccgccg ggtccgctcc
    11821 cgatgatggc gacgtcgaac tctttcaagc gacgaaactc cttctctttc gggcggcccg
    11881 aaggccgagc gcccggcgac gcactcgagc atcgcgaaac tcgtcggttg ggccttacgg
    11941 tgggaaagcg acaggtggcg cgaccgcgct tgcgtgccgc gcccgtccgg atgggtgcac
    12001 tatagcgtta gccccgacgg tcgcgcaagt ggcaagcccg actggctggc tcgctccgag
    12061 ggcctagcgg tagttcgcag gagaatcgcc gatcgcgacg atcgagcagg ccagcatggg
    12121 cgagaggccg agctccgcgg cgcgggcgac gacggcgtcg tcctcgctgc cgggattcaa
    12181 gaagagctgc ttcgggcgca gcttcgcgag gtcggggagc aggcggagcg tgaccgaagg
    12241 gggaacgtaa accaggacga cgtccagcgg ttccggaatc tgcaggacgg accgaaaggc
    12301 ggggaggcct tcgatctctt tctcgtggag gttgaccgga aaaacacgat ggccgtgcgc
    12361 gtggtaagcg cgcacggcct tgttcccgaa cttctcgcgg tcgcgggagg cgccgatgac
    12421 ggcgacgttc accgcgacgc ccccggtcga gccgaggcga cgtcgatcga gcgcgtgagc
    12481 ttctgtccgc tggcgagccc gtaggggccc gtcaacaccg cgggccggcg accgaattca
    12541 cgggcgtcgt cgaccgactc gaaccagatg tcggcccggt gctgccagcg gccgtgcatg
    12601 gaatcctcga ccacgaagtc gcccaggccg ttgatatgga cgatgtcccc gaagtcgaag
    12661 ggggctcccg gcgtgtagcg cttcaacagg tcgcgcgaca gcgcgaggac gccggggcgc
    12721 gtggacgtgt tggccgcagt gatgaacggg gtgtcatcgg tctcgcactc gcggctggtg
    12781 taggcgctga cgctcaccgt caccgcgacc tcgcggccgg tgcgaccctc ttcgcggtcc
    12841 ttgtagtagc gctcgagcgg cccccgcagc gaagcgactt cctcggccgg acgtccgtcg
    12901 accagctcgc cggccaggcg gatggaccgc gagccgtcgg taaggagaag ggcgatgggc
    12961 aacaggacca gaacgagccc ggtcccgatg gcggcgacac cccgggggcg aagccggtag
    13021 gcgggacgag ccgagagccg gaccgtcccc gcgaggcgac gcccaccttc gacatagaac
    13081 ccgaacgccc ccgatcggag gcgccaggtc agactcgacc atgcagttcg ttttctcatt
    13141 cggttgtccg gtcctcctct cgaccggccc attccaccgt cacgccgctt aaaagagcgg
    13201 cccgccggtg agtgcgggcc caatcgatag tggttccatc gaccccacta ggctacccgt
    13261 tcggtgttgc gctgtcaaag gaaaagtcgg ccgggagacc ccgctacagg gcgtccgtac
    13321 agttcaaagt cctattctag aggaagttag gtcgcctatc ttcgagccgt gcaggaggtc
    13381 attaggctaa ccccaagcgc tggggtaggg tccctaacgg ccggatgagg tgtcggtggc
    13441 gggcgagtac cgcggcgagg ggttatcccc gaaaaaatcg tccaggaacg cctcggccct
    13501 gatcccgggg aagtggatat aaacggggac acccagtttg ggatcgatca ggctctcgaa
    13561 cggatcgccc ccctcgatgc cgaccaccag cttgcgagcg gtcatcgacc ggtcggccga
    13621 ctcgaccacc gcctcccccg tgaaacccag ggtgacgacg gtggaggtga acgaccccgc
    13681 cagcaggagg gcccgggtgg aggggttctt gaactctcgg gcttgctgga gggccaggag
    13741 gatctcgatc tccatcgccc aatcctccca aaccacgacc ggccggggat tgacgctccg
    13801 tcgttgggcc ttcctcgggc cgttcaacga gaagtggccg aagaccgagt cccccgcgac
    13861 cgtgacgctg gcggtggctg gcatcccgcc cttggtcccc tcgtacgaaa acgaccgcag
    13921 cgcgtaggtg cgcgggtcag cctcggcctt ggtcgacaat tcgatcaccg cgctcccgtt
    13981 ctcgacccga agcgtggact cgaaacgcag cgcggtatcg gtctgggtga tgcgaacgtc
    14041 gcagcgtccc acccgctgcc cctgcacgta gaaggtgtaa cccgcgcgcg gcggcagcgg
    14101 ggtctcggcc gcctgggcgc cggtccacga aaggacgagg atgagcgccg aaaggcgcgc
    14161 ggtggcgaag tttcccatgt acgttagtgc tttctcccat ctggcgggca cacgacccgg
    14221 ggtgaactca ggggacgacg ggttccagta tagcacatgc cctttggcgg ccggcccctc
    14281 cgcggcttcc gcatcggatt tccgcgacgt ggtaccggag ttgcatgtgc tccgatggaa
    14341 agatggacgc gcccgtccgc gcggactacg ctacgccgaa tgagccgatt tccggtcctg
    14401 atgtaccacc gagttcgatc ctcccgcacg cccgttgccg acccggccga gcgcccgtgg
    14461 gcggtctcgc tcgaggcgtt cgagtggcag ctcgatcggc tgaaggcggc gggaaagatc
    14521 gcggtgacca tggcgcaggc ccacgccgcg ctcgggaccg gttccggcgt gccgcccgag
    14581 tgggtggtac taaccttcga cgatggaaac cagagcgatc acacgcacgc gcttccgctt
    14641 ctcaccgccc gcggattccg cgcgaccttc ttcgtatgcg gatgtcgcgt cggggaagcg
    14701 ggcggcttgg agcccgagat gatccgcgag atgcacgccg cgggcatgca ggtcggctcg
    14761 cacgcgatga cacaccggtt cctgaccacg ttgtccgcgc gcgacgaaga ggacgagctc
    14821 gcgcgctcga aggatctctt gcagggcatc gtggacacgc cggtcgatca ttttgcgccc
    14881 ccgggagggc gctggtcgaa gcgaaccgcg caggcgctgc gccgcctcgg gtaccgcgcg
    14941 gtttcgacct cggccttcgg atacaacgac gctcgcgagg ccaagttcgc ctaccggcgc
    15001 atcccggtgg tggacgcgac cacgcgcgcg cgcttcgacg acatcctcgc cggcgagcgc
    15061 tggaagctgg cggccggcta ccttcgcgcc ggttcgctcg gattggcccg acgcgcgatg
    15121 ggcgaagcga cctacgcgcg cctgcgcggc taccaggacc gttagcagag acgaaatgaa
    15181 gctggtcttc ttcatcagca tcgcgaccat cgtgtacgcg tacctgttgt acccggccat
    15241 catcgtggtg tgggcgcgcc tatttccgcg ccgtgtcgcc aagcgctacc gacaagtgcc
    15301 ggtgagcgtg gtcatcgccg cgcgcgacga agaggccaac atctcggcgc gcatcgagaa
    15361 cctgatcgcg caggagtacc cgcgcgccct gctcgaggtc gtggtggtct ccgacgggtc
    15421 gaccgaccgc accgccgagc tcgcgcgcca cttcgcgaac cacggcgtca acgtggtgga
    15481 gtgctcgtcg ccggtgggga aggcggcggc gctcaacatc ggtgtcgcca ccgcgtcgca
    15541 cggcatcgtc gtgttcgccg acgcgcgcca gcgcttcagc tcgaacgcga tcgcggagct
    15601 ggtgagcatg ttccacgacg agacggtcgg agcggtaagc ggcgagctga tcctgctctc
    15661 gggcgacgac gtgggcgagg tgcacgaggg cgtgggact
//
LOCUS       DASPGK010000061         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_600688, whole genome shotgun sequence.
ACCESSION   DASPGK010000061 DASPGK010000000
VERSION     DASPGK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600688"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VEC56_01610"
     CDS             complement(<1..184)
                     /locus_tag="VEC56_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 glycosyl transferase"
                     /protein_id="HXV12880.1"
                     /translation="MKHLLMIVPFFPPMGGGGVYRPLSFVRYLPEHGWRTTVIAPRGD
                     AFWIRDDSLATKIPPQC"
     gene            complement(181..1692)
                     /locus_tag="VEC56_01615"
     CDS             complement(181..1692)
                     /locus_tag="VEC56_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXV12881.1"
                     /translation="MTPRRSRFLGNTVALVLSTAIGTALALAQMKILAAALPLATFGL
                     FASLRGLSLLVAMLAANGFPQTLTRYLPEHAARSERAAASRATAGALTLTALLCAVLL
                     AAVVAARSVFFDKVPADALDARFILWFSVTTLAVAIKLVVYGGFNGLRRFGSQTVFET
                     GTLLAQVVWMALEADRLTLTRLFEIVGVTSLVAALVATPWLAGRLRRDVVPASTCAAP
                     ASYRDYWLGAAGLSVVALAFTDADRWVLSNVLALEALSLFHVASRVSRLANRFIALPV
                     LAFQPEVTRVASEGRTDVVASSTRAFFKASVVASVFAAAAIAVFAGDLILLASNESFL
                     GARPTLWLLAASIPLSAMTAPLTAVMKAFDGVRAALACDLVWACLYIVLLVTLAGPLG
                     VAGAGVAQLAACAAQFTLASRLASVKPRAREVVSSVAKALLCAAAAFGPVVVVEIVRA
                     PRALAWVLAIPAAWFYLRAARRLRIFTDDERARLSQALPGLGLATRRLGWWMP"
     gene            complement(1689..2645)
                     /locus_tag="VEC56_01620"
     CDS             complement(1689..2645)
                     /locus_tag="VEC56_01620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV12882.1"
                     /translation="MRVVVVDTFPPELDAVADASARATFYHTSTWLTSLAGAYPRLRL
                     RCLLAEEGGRALAYLPYFTTRRGPFESLWSLPFATYGGPVGDPTAAAELLAAYSRQLE
                     RRNVLEVNWVDYYNAFEPAGGEVEIALTHVVDIAPGFDVVWRERFDSSRRRRARRAEE
                     AGVVVRRATEASDVRSFVEVYRSRLERWEARSGHPEALFQSLLARGGERVGLYLATHQ
                     GDVVGGQINFYHRDTVTSWYGMASPRGNELHAGVLLYATFLREACEAGFGACNLGASL
                     GKESLIAFKRALGGEPHEYRSVTHRRRVGRLLAAARAWRRSR"
     gene            complement(2649..3857)
                     /locus_tag="VEC56_01625"
     CDS             complement(2649..3857)
                     /locus_tag="VEC56_01625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV12883.1"
                     /translation="MSVSRSSLPAIAGGTPVRSKPLPFFRAAVDEGDVAAVAETLRSG
                     WLTSGPKAVELEKRLGEYLGTSHTIAVSSCSEAMFLCMKALGVGPGDEVITSPITFAS
                     TVHAILHNGATPVLADIEHDTFGVDPGAVRACVSSRTKAIMPVHFGGQACRIEDVLAL
                     ADPAGIPVVEDAAHSFGARVHGRLLGGFGRATAFSFYATKNLTTAEGGAITTEDEVLA
                     QRLRLLGYHGMSRDSWSRYSDKGSWYYQVQIPGYKCNLSDLHAALGLTQLAKIDLLLE
                     RRRAVADALTRALGACAAIDLPREYPGYWHTWHLYVIRVRPEALRIARDEFIQALRAE
                     NVGTSVHFIPIHRHPFFAPYLRPGQAFPAADDYYARCISLPIFPDMTDDDVRDVGEAV
                     TRIAEHFRAG"
     gene            complement(3854..5314)
                     /locus_tag="VEC56_01630"
     CDS             complement(3854..5314)
                     /locus_tag="VEC56_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HXV12884.1"
                     /translation="MVKRLLIVGAGEAGRMLAREVGSRARDQYLAVGFLDDAPALAGH
                     DVDGLPVLGTTDALQHVVRAQGVDEVLIAVPSAGRDFVRRMVASCRDAGVAYRIAPGL
                     LEIIKGPVHLEQIRDVRPEDLLGRETVEFDEGEIARFLNGRTVLVTGAGGSIGGEICR
                     QVGRFEIERLVLLGRGENQIYEIERELRTLYPTRDIVPVIVDVRDADALARTFSLYEP
                     EIVYHAAAHKHVHYMEAFPAEAVKNNILGTRNVIDAAYAAAVGRVVMLSTDKAARPGG
                     VMGATKRLAEYLMAARNNPAGSPKLVSVRFGNVLASRGSVVPLFVAQIRAGGPVTVSH
                     EDATRYFMSLKEACMLVIQASLMGEGGEIFILRMGEPIRIAELARDLIALSGHKAGDI
                     RIEFTGLRPGEKLHESLVAEGEETIASRHENILRTASRVPPRAELDAALETLATLAAA
                     GDDDAIRAELARIIADAGMSGTPRAGAAKGKPVKRP"
     gene            complement(5322..>5740)
                     /locus_tag="VEC56_01635"
     CDS             complement(5322..>5740)
                     /locus_tag="VEC56_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV12885.1"
                     /translation="LRYPLSIISPLSNSDCWALAMHPTEDILYVGTIAGVTVLDYTPA
                     PAVATNLSRVYLYPNPVYRSRGHNEIMIQNITGPVSIEVYNLEGVLVHSQTVDANGQV
                     AWDLTTETGFLVGSGNYLVRVVGPDGAVTKTISVLR"
BASE COUNT          974 a         2016 c         1970 g          780 t
ORIGIN      
        1 ggcattgggg cggtatcttc gtggccaggc tgtcgtcgcg gatccagaag gcgtcgccgc
       61 ggggcgcgat caccgtcgtt cgccacccgt gctcgggcag atagcgcacg aacgagaggg
      121 gccggtacac gccgccgccg cccatgggcg gaaagaaggg cacgatcatg agcaggtgct
      181 tcacggcatc caccacccca gccggcgcgt cgcgaggccg aggcccggga gggcctgcga
      241 aagtcgcgct cgctcgtcgt cggtgaagat acgcagccgg cgcgcggcgc gcaggtagaa
      301 ccacgccgcc ggtatcgcca gcacccacgc gagtgcgcgc ggggcacgca cgatctcgac
      361 caccaccacc ggcccgaacg cagccgccgc gcacagcagt gccttcgcga ccgacgacac
      421 gacttcgcgg gcgcgcggct tcaccgacgc caagcgagac gcgagcgtga attgcgccgc
      481 gcacgccgcc agttgcgcga cgcccgcacc ggccaccccc agcggtcccg ccagcgtgac
      541 caacagaacg atatacagac acgcccacac caggtcgcac gcgagcgcgg cgcgcacccc
      601 gtcgaatgcc ttcatgaccg cggtcaaggg cgcggtcatg gccgaaagcg ggatggatgc
      661 ggccagcagc cacagcgtgg gacgcgcgcc cagaaaactc tcgttcgacg ccagcagaat
      721 gaggtcgccg gcgaacaccg cgatggcggc cgcggcgaag accgacgcca ccacgctggc
      781 cttgaagaag gcccgcgtcg acgacgcgac cacgtcggtg cgcccctcgc tcgcgacgcg
      841 tgtaacctcg ggctggaacg cgagcaccgg gagcgcgatg aatcgattgg cgaggcgcga
      901 tacgcgggag gcgacgtgga ataacgaaag cgcttcgagt gcgagcacgt tggacagcac
      961 ccagcggtcg gcgtcggtga acgcgagggc taccacactc aagcccgcgg cgcccagcca
     1021 gtagtcgcgg tacgacgccg gagcagcaca cgtggacgcc ggcacgacgt cgcggcgcag
     1081 ccggcccgcg agccacggtg ttgccacgag tgccgccacc aaagaggtga cgccgacgat
     1141 ctcgaagagg cgcgtcaggg tgaggcgatc ggcttcgagc gccatccaca cgacttgcgc
     1201 gagcagggtg ccggtctcga agacggtctg cgagccaaag cgccgcagcc cgttgaagcc
     1261 gccgtacacc acgagcttga tcgcgaccgc gagcgtcgtg accgagaacc aaagaatgaa
     1321 tcgcgcgtcc aacgcatccg cgggcacctt gtcgaagaag accgagcggg ccgcgaccac
     1381 agccgccagc agtacggcgc acaagagcgc ggtgagtgtc agagcgcccg cggtcgcgcg
     1441 cgacgcggcc gcgcgttcgc tgcgcgcggc gtgctcagga agataccgcg tcagcgtttg
     1501 cgggaatccg ttcgcggcca gcatcgcgac cagcagcgag agcccgcgca gcgacgcaaa
     1561 caacccgaag gtcgcgagcg gcagcgccgc ggccaggatc ttcatctgcg cgagcgcgag
     1621 cgcggtcccg atcgcggtcg acaagaccag cgcgaccgtg ttgccgagaa agcgcgagcg
     1681 gcggggcgtc atcgcgaccg ccgccacgcg cgcgcggccg ccagcaggcg tcccacccgg
     1741 cgccggtgtg tcaccgagcg gtactcgtgg ggctcgccgc cgagggcgcg cttgaacgcg
     1801 atcagcgact ccttgcccag gctcgccccc agattgcacg cgccgaagcc cgcctcgcac
     1861 gcttcgcgca agaaggtcgc gtacagcagc acgcctgcgt ggagctcgtt gccgcgcggt
     1921 gacgccatgc cgtaccacga cgtcaccgtg tcgcggtggt agaaattgat ctgtcccccc
     1981 accacgtcgc cctggtgcgt cgccaggtaa agccccaccc gctcgccgcc gcgcgcgagg
     2041 agcgattgaa acaacgcttc ggggtgtccg gaccgcgcct cccagcgctc gagacgcgag
     2101 cggtatacct cgacgaagct gcgcacatcc gacgcctccg tcgcgcgccg caccaccaca
     2161 cccgcctcct cggctcgtcg cgcgcgacgg cggcgcgagc tatcgaagcg ctcgcgccac
     2221 acgacgtcga atcccggcgc aatgtcgacc acgtgggtca gggcgatctc cacctcgccg
     2281 cccgcgggct cgaacgcgtt gtagtagtcg acccaattga cttccagcac gttgcgtcgt
     2341 tcgagctggc ggctatacgc cgcgagcagc tccgcggccg ccgtggggtc gcccaccggc
     2401 ccgccgtaag tcgcgaacgg cagcgaccac agcgactcga acggcccgcg ccgggtggtg
     2461 aaatacggaa ggtacgccag cgcgcgcccg ccctcctcgg ccaagaggca gcgcaagcgc
     2521 aggcgcgggt aagcgccggc cagcgacgtg agccacgtcg acgtgtgata aaacgtggcg
     2581 cgcgcggacg cgtcggcgac cgcgtccagc tcgggcggga acgtgtcgac gaccacgact
     2641 ctcatcggct agcccgcgcg gaagtgctcg gcgatacgcg tcaccgcttc gcccacgtcg
     2701 cgcacgtcgt cgtcggtcat gtcggggaat atgggaagcg agatgcagcg cgcatagtag
     2761 tcgtcggccg cggggaacgc ctgcccggga cgcaggtaag gcgcgaagaa cgggtggcga
     2821 tggatgggaa tgaagtgcac cgacgtgccc acgttctccg cgcgcagcgc ttggatgaac
     2881 tcgtcgcgcg cgatgcgaag cgcctcgggc cgcacgcgaa tcacgtacag gtgccaagtg
     2941 tgccagtagc ccgggtactc gcgcggcaga tcgatggccg cgcaagcgcc cagcgcgcgc
     3001 gtgagcgcgt ccgccaccgc ccgccgccgc tcgagcaaga ggtcgatctt cgcgagctgc
     3061 gtcaatccca gcgccgcgtg caaatcgctc aggttgcact tgtagccggg aatctgcacc
     3121 tggtagtacc acgatccctt gtccgagtaa cggctccagc tgtcgcgcga catgccgtgg
     3181 tagccgagca aacgcagccg ctgtgcgagt acctcgtcct cggtggtgat ggcgccgccc
     3241 tcggcggtgg tgagattctt ggtcgcgtag aagctgaacg cggtggcgcg cccaaagccc
     3301 cccaacaacc gcccgtgcac gcgcgcaccg aacgagtggg cggcgtcttc gaccaccggg
     3361 atgccggccg ggtccgcgag ggcgaggacg tcttcgatgc ggcacgcctg cccgccgaag
     3421 tggaccggca tgatggcctt ggtgcgcgac gatacacacg cgcgcaccgc gcccgggtcg
     3481 acgccgaagg tgtcgtgctc gatgtcggcc agcacgggtg tcgccccgtt gtggagaatc
     3541 gcgtgcacgg tggatgcgaa cgtgatgggc gaggtgatga cttcgtcgcc gggtccgacc
     3601 ccgagcgcct tcatgcacaa gaacatcgcc tcgctgcacg agctgaccgc gatggtgtgc
     3661 gacgtcccca ggtactcgcc caggcgcttc tccagttcga ccgccttggg tcccgacgtc
     3721 aaccaacccg accgcagcgt ttcggccacc gcggccacgt cgccttcgtc gaccgccgcg
     3781 cggaagaacg gcagcggttt cgagcgcacg ggggttccgc ccgcgatcgc gggcagcgaa
     3841 gagcgcgaga cgctcacggg cgtttcaccg gcttcccttt cgccgcgccc gctcgcggcg
     3901 tgccggacat gcccgcgtcc gcgatgatgc gcgcgagctc ggcgcgaatc gcgtcgtcgt
     3961 cgccggccgc ggccagcgtc gcgagcgttt ccagcgcggc gtccagctcg gcgcgcggcg
     4021 gcacgcgcga ggccgtgcgc aggatattct cgtgccgcga cgcaatcgtc tcctcgcctt
     4081 ccgcgaccag cgactcgtgc agcttctcgc ccggtcgcag ccccgtgaac tcgatgcgaa
     4141 tgtcccccgc cttgtggccc gagagcgcga tcaggtcgcg cgccagctcc gcgatacgaa
     4201 tcggctcgcc catgcgcaga atgaagatct ctcccccttc gcccatcagc gaggcctgga
     4261 tcacgagcat gcacgcttcc ttgagggaca tgaagtagcg cgtggcgtcc tcgtggctca
     4321 ccgtcaccgg accgcccgcg cgaatctggg ccacgaacag cggtaccacg ctgccgcggc
     4381 tggcgagcac gttgccgaag cgcacggaga cgagcttggg cgacccggcc gggttgttac
     4441 gcgcggccat caggtactcg gcgaggcgct tggtggcacc catgacgccc cccgggcgcg
     4501 ccgccttgtc ggtcgagagc atgacgacgc gcccgacggc ggcggcgtag gcggcgtcga
     4561 tcacgttgcg cgtccccagg atgttgttct tcacggcctc ggccggaaac gcctccatat
     4621 aatggacgtg cttgtgggcg gcggcgtgat aaacgatctc gggctcgtag agcgagaagg
     4681 tgcgggcgag cgcgtccgcg tcgcggacgt cgacgatgac cgggacgatg tcgcgcgtgg
     4741 gatagagggt acgcagctcg cgctcgatct cgtagatctg attctcgccg cggcccaaca
     4801 gaaccagccg ctcgatctcg aagcgcccca cctggcggca gatctcgccc ccgatggagc
     4861 cgcccgcgcc cgtgacgagg acggtgcggc cgttcaggaa gcgcgcgatc tcgccctcgt
     4921 cgaactccac cgtctcgcgc cccaagagat cctcgggccg tacgtcgcga atctgctcca
     4981 agtggacggg acccttgatg atctccaaga gtccgggtgc gatgcgatac gcgacacccg
     5041 cgtcgcggca cgacgccacc atgcggcgca cgaagtcgcg cccggccgac gggaccgcga
     5101 tcaatacctc gtcgacgcct tgcgcgcgca ccacgtgctg gagcgcgtcg gtggtcccga
     5161 gcaccggcag tccgtcgacg tcgtgccccg cgagcgcggg ggcgtcgtcc aggaatccga
     5221 ccgcgaggta ctggtcgcgc gcgcgcgagc cgacttcgcg agccagcatg cggcccgcct
     5281 cgcccgcgcc gacgatgagg aggcgtttca ccatcgagat tctagcgcag caccgagatc
     5341 gtcttggtga cggcgccgtc cggacccacc acgcgcacca ggtagttgcc gctgccgacg
     5401 aggaagccgg tctcggtggt gagatcccac gccacctgtc cgttggcgtc gacggtctgc
     5461 gaatggacca gcacgccttc gaggttgtag acctcgatcg acaccggccc ggtgatgttc
     5521 tggatcatga tctcgttgtg cccgcggctg cggtacaccg gattcgggta caagtagacg
     5581 cgcgagaggt tggtcgcgac cgccggtgcc ggcgtgtagt cgagcacggt gacgccggcg
     5641 atggtgccga cgtagaggat gtcctcggtc gggtgcatcg cgagggccca gcagtccgag
     5701 ttcgacagcg gcgagatgat gctcaatgga taccgcagcg
//
LOCUS       DASPGK010000062         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1420108, whole genome shotgun sequence.
ACCESSION   DASPGK010000062 DASPGK010000000
VERSION     DASPGK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420108"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VEC56_01640"
     CDS             complement(<1..636)
                     /locus_tag="VEC56_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12886.1"
                     /translation="MRIGAVLLMTCAVLIPIGARADSTSVWEGNVRVGGAIEDEEGDQ
                     SVMQETFNIHEGVTFTRLFLNGRFNRDTHLHFDADNLTLDGRRANFDLRRTGVGRLRS
                     HYDESDYIFDPAGSVLATRRDWWSTMSLTPRRWLWISGDYGLQTRRGDRIGFPDNTAS
                     ALGTAYDSDLNRWRVQVQGNHGSGAAGTFAYDGVALTDKLDARNERDGTVFS"
     gene            complement(650..1588)
                     /locus_tag="VEC56_01645"
     CDS             complement(650..1588)
                     /locus_tag="VEC56_01645"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV12887.1"
                     /translation="MKTASGVAILTFLAAIGAALVAFAFQSAESSEPSTLTDTVCMDC
                     HEDVAHGLMGTAHQLVSAKVSCLDCHAGAATEAHADDPDLKPVNPAHLPADSLTALCA
                     TCHADAHALNVFERDPHRDGGLACAACHSVHASKTQMGLLVDEEPQLCFSCHASQKGD
                     FAMPTHHPVIEGVVRCSDCHVSVAQSAKQHVPSGPSETCTLCHAMFQGPYPFQHQAAV
                     DYSTEEGGCLNCHAPHGSPNPRLLKQSYQSPDYALCMQCHSVPKHEFNINHGAQWAGM
                     PCADCHVDVHGSYDNAKLLDPALQSQGCFAVGCHSY"
     gene            complement(1760..>2155)
                     /locus_tag="VEC56_01650"
     CDS             complement(1760..>2155)
                     /locus_tag="VEC56_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12888.1"
                     /translation="CLDCHKEGEKGFEAAGKIHAELAGLSTTYANAVAAQDEVRRIGM
                     DDVDIGFMLQDAHQSLIQARTLVHTFDPARVKTTTDEGARKATEALEAAAAQVRDFHA
                     RRRGFGMATLFTTVLVIALFLRIRQIESR"
BASE COUNT          371 a          689 c          697 g          398 t
ORIGIN      
        1 ggaaaacacc gttccgtcgc gctcgttgcg cgcatcgagc ttgtccgtga gcgccacgcc
       61 gtcgtaggcg aaggtgccgg ccgcacccga cccgtgattg ccttgtacct gcacgcgcca
      121 gcgattcagg tccgaatcgt aggcggtgcc cagcgcgctc gcggtgttgt cggggaaccc
      181 gatgcggtcg ccgcggcggg tctgcaggcc gtagtcgccc gagatccaca gccaccggcg
      241 cggagtgagc gacatggtcg accaccagtc gcggcgcgtc gccaagaccg agcccgcggg
      301 gtcgaagatg taatcgctct cgtcgtagtg cgaccgaaga cgtcccacac cggtgcgccg
      361 caggtcgaaa ttcgcgcgcc gtccgtccag ggtgagattg tcggcgtcga aatgcagatg
      421 cgtgtcgcga ttgaagcggc cgttcaggaa caacctggtg aaggtgacgc cctcgtggat
      481 gttgaaggtc tcctgcatca ccgactggtc gccttcctcg tcctcgatgg caccgcccac
      541 gcgcacgttt ccctcccaca cggaagtcga gtcggcacgc gcgccaatcg gtatcagcac
      601 cgcgcacgtc atcaacagca ccgctccgat tcgcatcgcc gtcgtctccc tagtacgagt
      661 ggcacccgac cgcgaagcac ccctgcgact gcagcgccgg gtccaggagt ttcgcgttgt
      721 cgtaagatcc gtgaacgtcg acgtggcaat ccgcgcacgg cattcccgcc cactgtgccc
      781 cgtggttgat gttgaactcg tgcttgggca cggagtggca ctgcatgcac agcgcgtagt
      841 ccggcgactg gtagctctgc ttgagcaggc gcgggttggg cgacccgtgg ggggcgtgac
      901 aattcaggca accgccctct tcggtcgagt aatcgacggc ggcctggtgc tggaacgggt
      961 atgggccctg gaacatggcg tggcagaggg tgcacgtctc ggacggcccg ctcggcacgt
     1021 gctgcttcgc actctgcgcc accgaaacgt ggcagtcgct gcagcgcacc acgccctcga
     1081 tcaccgggtg gtgcgtgggc atcgcgaagt cacccttctg cgacgcgtga cacgagaaac
     1141 acaattgcgg ctcttcgtcg accaggagcc ccatctgcgt cttgctcgcg tgcacgctgt
     1201 gacaggcggc gcacgcgagg ccgccgtcgc gatggggatc gcgctcgaat acattcaggg
     1261 cgtgcgcgtc ggcgtggcac gtggcgcata gcgcggtaag agagtccgct ggcaggtgcg
     1321 cggggttcac cggcttcagg tcgggatcgt cggcgtgcgc ttccgtggcc gcgccggcgt
     1381 gacagtccag gcacgagacc ttggccgaca ccagttgatg cgcggtgccc atcaatccgt
     1441 gggcgacgtc ctcgtgacag tccatgcaca cggtgtcggt aagagtgctc ggctccgagg
     1501 attcagcgct ctgaaacgca aacgcgacca gcgcggcccc gatggcagcc aggaaggtga
     1561 gaatcgcaac gccggatgcg gttttcatag atttcggatc actatctccg aagaagtata
     1621 tcgacgtccc aaaaagccgt ctagcgcgaa gtacacatgg agcctagttt tgcagagttt
     1681 caaataaaag aagtttgtag tctatttttg ctccggttct atcgtgtggc ggcgacgtgt
     1741 agacaccgaa gttccaacac tagcgcgatt ctatctgccg aatgcgtagg aacagggcaa
     1801 tcaccaggac cgtcgtgaac agcgtggcca ttccaaagcc gcgacgccgc gcgtgaaagt
     1861 ccctgacctg cgccgccgcc gcctcgagcg cctcggttgc cttgcgcgcc ccctcgtccg
     1921 tcgtggtctt gacgcgcgcg gggtcgaagg tgtgaaccag cgtgcgcgct tggattagac
     1981 tctggtgtgc gtcctggagc atgaagccga tatcgacgtc gtccattccg atgcgccgca
     2041 cctcgtcctg cgcggcgacg gcgttggcgt acgtcgtgct caggccggcc agctcggcgt
     2101 ggatcttccc cgccgcctcg aagcccttct cgccttcctt gtgacaatcg aggca
//
LOCUS       DASPGK010000063         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_15331, whole genome shotgun sequence.
ACCESSION   DASPGK010000063 DASPGK010000000
VERSION     DASPGK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15331"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1267)
                     /locus_tag="VEC56_01655"
     CDS             complement(140..1267)
                     /locus_tag="VEC56_01655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV12889.1"
                     /translation="MKVCIVSLNIVPLFAGERGGYFGGAEVQAVFVAEVLEACGQHVS
                     LVVADLSEPELIPFATQNAYHSAAGVRGLRFFHPRWTGVMRALERADADVYYQRNAGM
                     ITGLVAMFCKRNGKLFVYGAGSDVDFSPRDVAIRGARDRLIYNWGLKASRGFVVQNQA
                     QRAAAARFHKPTRVIPNGVTPIDVASDDTDGTIVWIGAMWRVKRPGLLLELARRMPDR
                     RFVVIGSGGELAKSIEREAARLPNVEMRGRLGRDEVSKVLRKAALLVNTSSVEGFPNA
                     FLEAWNHGVPVVAFNDVDGIIAEAGVGAVCSNLDEMVRAISDLTGDRGHAAAVRARAR
                     RLVSERFSPAVLGPRYVEFFQELLSGSEPAAVEGAVAVRRS"
     gene            1352..3877
                     /locus_tag="VEC56_01660"
     CDS             1352..3877
                     /locus_tag="VEC56_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12890.1"
                     /translation="MMHPSRLIPRAPLLVALATGLYAFVPMLPGRLAGPLGIAVVLLM
                     CVVVPGVAVMRATRLRVDDPLEAVAHTCASGFVFWLVCTFVWVLSGASLDAFRLALPA
                     TIVALWALVPTRDTTRVDPLRTPIRPRDRRFIVALALLVAMAAFGVAVVGPPLAITND
                     TIDHAGYVSEIARTGDPFPTSAIYWNAGEDGEDFRKALLHSIYGFTARHTGAPPIDVL
                     AAYGAFLLAVMALVVYAAARALFKRHRLPAVLAAVIFLVGTDWGVGNPMVRAAFYPNR
                     FGTAFLLLFITAAVEYVHRGPTTALRWCVAYAFAATAIHVQFAVIVPIAAAVLVPWRT
                     CSPCSSWGEHLTRALRVVAAAVIGAFPFALYRYLTAYRSNPLHEQVQGAVFLTDRWFI
                     ADPARLWHTFGPIGLAALVCIPALWKKRRDVPGVGYAIASFVTLLVVQLVPFILTPLY
                     AAVKYLAFRLDVLVPFYLLPAYFIATRPRRARFAMGVVLVALAAVVLPLAHHSAFSPS
                     VLSAERRVGPDRWARGLVQLAGALPRGSIVASDPVTSYLMSAFTPYHVVCTLDQHAPP
                     NDLRVEERMSAARDIVSPFTSAREKEALIQGYEVSHVVVNETLPENMVLNYWTLDPAD
                     AHDAEEMFRSLRYEFETIPLSDGLTAFRWKHDERLATLPRPVPRPVVEALPGNAQPIG
                     VRSGEATLEGVVLHGAGILPAGGELVLDLYWSRDRELPPGTYVVTIRFDRKDLDLPFD
                     GKPFPKITRKVVERWKRERYRFRADHMIQGGLFAPDAWRMGDIVEDDVRIAVPTDIAP
                     GRYRVEAKMLRVANQPNHRLRDYFFDDDSYSGVQIGEVTIRRW"
     gene            3941..>4138
                     /gene="queF"
                     /locus_tag="VEC56_01665"
     CDS             3941..>4138
                     /gene="queF"
                     /locus_tag="VEC56_01665"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXV12891.1"
                     /translation="MPNLPSKELEVFPNPHPDRDYLISFECPEFTCLCPRTGQPDFAT
                     IRIRYVPNERCVELKSLKLYLW"
BASE COUNT          631 a         1465 c         1298 g          744 t
ORIGIN      
        1 ggcgtggttc caaaggccct cgtcgtcggg gccccggtgg cgcaagcacg cgaccgctcg
       61 cgcgagatct cgctcgagtg ctccggcgtc tccaccgcgc gtcacaaatc ccgctattcc
      121 gcacataaat cgagttggct caggatcgac gaaccgcgac ggcaccctcg acggccgccg
      181 gttcactccc cgacaacagc tcttggaaga actcaacgta acgcgggccc agtacggcgg
      241 gcgaaaaccg ttcgctcacg agtctgcgcg cgcgggcccg aaccgcagcc gcgtgccccc
      301 ggtcgcccgt gagatcggag atggccctga ccatctcatc gagattggag cacaccgcgc
      361 cgacccccgc ctcggcgatg atgccgtcga cgtcgttgaa cgcgaccacg ggcaccccgt
      421 gattccacgc ctcgaggaac gcgttgggga atccttcgac ggacgacgta ttcaccaaca
      481 gcgcggcctt gcgaaggacc ttgctcacct cgtcgcgccc caaccgcccc cgcatttcga
      541 cgtttggcag ccgcgcggcc tctcgttcga tcgacttcgc caattctcct ccgctcccga
      601 taacgacaaa gcgccggtcg ggcatgcgac gcgccaactc gagcagcaac ccgggacgct
      661 tcacgcgcca catggcgccg atccatacga tcgtgccgtc ggtgtcatcg gatgcgacgt
      721 cgatcggcgt gaccccgttg ggtatgaccc gcgtcggctt gtggaaacgc gcggcggcgg
      781 cgcgctgcgc ttggttctgc accacgaagc cccggctcgc tttgagcccc cagttgtaga
      841 tcagccggtc gcgcgcgccg cgaatcgcga cgtcgcgcgg ggaaaaatcg acgtcgcttc
      901 ccgcaccgta cacgaatagc tttccgttcc tcttgcagaa catggcgacc agccccgtga
      961 tcatgccggc attccgctgg tagtaaacgt cggcgtccgc gcgctcgaga gcgcgcatca
     1021 cgccggtcca ccgcgggtgg aagaaacgaa gccctcgcac gcccgccgcc gagtgatagg
     1081 cgttttgcgt tgcgaaggga atgagctcgg gttcggagag atcggcgacc acgaggctga
     1141 cgtgctggcc gcacgcctcg agcacctcgg ccacgaagac cgcctggacc tcggcacctc
     1201 cgaaatagcc gccacgctcg cctgcgaata aggggacgat gttgagcgaa acgatacaga
     1261 ccttcatact gccgtggcct tcaagtttgt tattctagca cgtcgcgata tctgctataa
     1321 acacaaaacc ttaggcggtc cctagaccct cgtgatgcac ccatctcgac tgataccgcg
     1381 ggctcccctc ctggtcgcct tggcgacggg cctgtacgcg ttcgttccca tgctccccgg
     1441 ccgcctggcg ggaccgctgg gaatcgccgt ggtcttgctg atgtgcgtgg tcgtgccggg
     1501 agtcgcggtg atgcgtgcca cccggcttcg tgtcgacgat ccgttggagg ccgtggccca
     1561 tacctgcgcg agcggattcg tcttctggct cgtgtgcacg ttcgtctggg tgctctcggg
     1621 cgcctcgctg gatgcgttcc gcctcgccct cccggccact atcgtcgcct tgtgggcgct
     1681 cgtaccgacg cgggacacga cgcgggtcga tccgctacgc actcccattc ggccccggga
     1741 ccggcgcttc atcgtggcgc tggcactgct ggtggcgatg gcggccttcg gcgtcgccgt
     1801 ggtgggtccg ccgctcgcga tcaccaacga caccatcgac cacgccggct atgtgtccga
     1861 gatcgcgcgc accggggatc ccttcccgac ctcggccatc tattggaacg cgggcgagga
     1921 cggagaggat tttcgcaagg cgctattgca ctcgatctac ggcttcaccg ctcgccacac
     1981 cggcgcaccg cccatcgacg tgctggccgc gtacggggcc ttcctgctcg cggtgatggc
     2041 gctggtcgtg tacgcggcgg cgcgcgcgct gttcaagcgc catcgccttc ccgccgtgct
     2101 ggcggccgtc atcttcctgg tcggaaccga ctggggcgtg ggcaacccga tggtgcgggc
     2161 ggcgttctat cccaaccggt tcgggaccgc cttcctcctg ctcttcatca ccgcggcggt
     2221 cgagtacgtc catcgcggcc ccaccacggc gctgcgctgg tgcgtcgcgt acgcgttcgc
     2281 ggccacggcg atacacgtac agttcgccgt gatcgtgccg atcgcggccg ccgtgctggt
     2341 cccctggcgc acgtgttcgc cgtgttcgag ctggggcgag cacctgactc gagcgttgcg
     2401 cgtggttgcg gccgccgtca tcggggcgtt tcccttcgcg ctctaccggt acctcaccgc
     2461 gtatcgcagc aacccgcttc acgagcaggt ccagggcgcc gtattcctca ctgaccgttg
     2521 gttcatcgcg gacccggcgc gcctgtggca cacgttcgga ccgatcgggc tggccgcgct
     2581 ggtctgtatc cccgctttgt ggaagaagcg acgcgacgtg cccggggtcg gctacgccat
     2641 cgcgtcgttc gtaacactct tggtcgtgca gctggtgccg ttcatcctga cgccgctcta
     2701 cgcagccgtg aagtacctcg cgttccgact cgacgtcctg gtgccattct acttgctgcc
     2761 cgcgtacttc atcgcgacgc gcccacgccg ggcccgcttc gcgatggggg tcgtgctggt
     2821 ggcgctggcg gcggtcgtgc tcccgctcgc gcaccactcc gcgttctcgc cctcggtgct
     2881 gtcggccgag cggcgggtcg gacccgaccg ctgggcgcgc gggttggtgc agctcgcggg
     2941 cgcgttgccg cgcggatcga tcgtcgccag cgacccggtg acgtcgtacc tcatgtcggc
     3001 cttcacgccc taccacgtcg tgtgcacgct cgatcagcac gcgcccccca acgacctgcg
     3061 ggtcgaagaa cgcatgtccg cggcgcgcga catcgtgagc ccattcacgt ccgcgcgcga
     3121 gaaggaggcc ctgatccagg gctacgaggt ctcccacgtc gtggtcaacg agacgctgcc
     3181 ggagaacatg gttctcaact actggacgct cgacccggcc gacgcgcacg acgccgagga
     3241 gatgttccgt tcgctccgct acgagttcga gaccattccg ctctccgacg ggctcacggc
     3301 cttccgatgg aagcacgacg agcgcctggc cacgctgccg cggcccgttc cgcgcccggt
     3361 cgtcgaagcg ctgccgggga acgcgcaacc catcggcgtg cgctccggcg aagcgacgct
     3421 cgaaggcgtc gtcctgcacg gcgccggcat cctgcccgcg ggcggcgagc tggtgctgga
     3481 cctctactgg tcgcgcgacc gcgagcttcc gcccggcacc tacgtcgtca cgattcgctt
     3541 cgaccgcaag gacctcgatc tgccgttcga cggcaaaccg ttcccgaaga tcacgcgcaa
     3601 ggtcgtcgag cgatggaagc gcgagcgcta tcgctttcgt gccgaccaca tgatccaggg
     3661 cgggctgttc gcgcccgacg cgtggaggat gggcgacatc gtcgaggacg acgtgcgcat
     3721 cgcggtgccg accgacatcg ccccgggtcg ttaccgcgtg gaggccaaga tgctccgcgt
     3781 cgccaaccag ccgaatcatc ggctgcgcga ctacttcttc gacgacgact catactcggg
     3841 cgtacagatc ggggaagtga caattcgccg ctggtgatac gctgcgcgaa tgcgcagcgc
     3901 cttgcattga cgaagctgcg cgcgttacaa ttcgttgacc atgcccaacc taccctcgaa
     3961 ggaactcgaa gtatttccga acccacaccc cgaccgggac tacctgatct cgttcgagtg
     4021 ccccgagttc acgtgcctgt gcccgcgcac cgggcagccc gacttcgcga ccattcgcat
     4081 tcgctatgta ccgaacgagc gctgcgtgga gctgaaatcg ttgaagctct acctatgg
//
LOCUS       DASPGK010000064         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_250991, whole genome shotgun sequence.
ACCESSION   DASPGK010000064 DASPGK010000000
VERSION     DASPGK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250991"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VEC56_01670"
     CDS             complement(<1..481)
                     /locus_tag="VEC56_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV12892.1"
                     /translation="MPKTILIIDDEKTIRWSLAEAIAAGGYDTVDAETAADGVAKFRD
                     ASPDLVLLDMKLPDGSGLDVLKMVKAEDPSVPVVMMTAYAEVETAVEAMKLGAYDFVA
                     KPYSVDKLRVTISNALENQRLRSEIAYLRSGSTGNAFFKQFIGRSPAMVEIFDKIKKI
                     "
     gene            complement(513..2153)
                     /locus_tag="VEC56_01675"
     CDS             complement(513..2153)
                     /locus_tag="VEC56_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV12893.1"
                     /translation="MSSASPDPRVDGRPGPAPAQTPAAPERLRPLAAQLKTLYHMGRD
                     LGEDENWSDALDRFLMALVSFMEAEGAGLLLLSDGTRSLSARAVFHLDEGHVGRAIDT
                     IRDGWTRHARAAEIHALESYGSSRPTSCLERTAAWRTTVIPLRHRGRALGFLILDKAY
                     RRAHHFQGDYLFLSTLQTIFAEEIANASYISELRQLSRFNHKVLDNLRSGVVTTDLDG
                     AIRFANAWAAEMCPRLRGRGSAGTVHMDELFQHAIHGGLFRRLLEAADDAEGLEVTCH
                     GGRAPFPVRLRVTKMHDDNINGTVLVAIFDDLSEMKRMEEAVRRNDRLRALGQLSAGV
                     AHEIRNPLAGIATTAEVLGEKLAGDDDKVRYIHAMLDEIQRLDGIVKNLLAFARPPKP
                     QLSRCGVADVAARVVSLSADAATARGVVVATSVDADVICLADPSQLTQVLLNLVLNAV
                     QACGHGDRVAVAARRDEGWVAIEVADTGAGVAEEVRATLFDPFVSTKTQGTGLGLAIS
                     RQIVDDHAGTIACEFLEKGTKFTVRLPALAVPVAGAPA"
     gene            complement(2382..2735)
                     /locus_tag="VEC56_01680"
     CDS             complement(2382..2735)
                     /locus_tag="VEC56_01680"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV12894.1"
                     /translation="MTRVERRSAARVPARLAMEITLGGGRARTESLNVSANGVYFAST
                     SFIPLLTKLRITLELPEDGADPSQVTCDGVVVRTEPEVETAGEDDYQVACYFTDISDR
                     DRERLESYILRHVPF"
     gene            complement(2949..3233)
                     /locus_tag="VEC56_01685"
     CDS             complement(2949..3233)
                     /locus_tag="VEC56_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV12895.1"
                     /translation="MTKADLVEEISRVTGLNKKDTSIIVNEVITNICRALAEGDKVEL
                     RGFGSFKVKERRARQARNPRTGDGVQVPAKLVPFFKASNELKARVNDTNA"
     gene            complement(3791..>5199)
                     /locus_tag="VEC56_01690"
     CDS             complement(3791..>5199)
                     /locus_tag="VEC56_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HXV12896.1"
                     /translation="TTDEPPARIARAFEQVGPRVSGRICIFEDGKFANFFPLSLAQPV
                     FDLRVGAGTLRERWLAGGTHGQVSLICRDYLADVVRLAAPGLPVNDAVAGDTLLVNGR
                     WLCMAGERDAFLSRLPDDSVALQGGCLVAARVSRDAAADLARALRAQITDAAVDAVCS
                     QLEKARAQSAADADAEALSPDLMRVIDARRLRRVEMPEARVLSFPWQLVEHNSACIAD
                     DFARAARGHAKDAAIHPGVHLVEPKRIAIGSRAAIRAGTVIDASDGPVVIGEGVVVMA
                     NATIVGPAAIGAGSVVKAGARILAGTSVGKVCKVGGEVDESIFADYTNKQHDGFLGHT
                     YLGSWVNIGAASNNSDLKNNYGSVRMWNAGRPRDTGRQFLGLVMGDHTKTGINAVFNT
                     GTVVGFNCNLYSSEMPDAFVPSFSWGHGRDVVPYELEKAVQTATAAMHRRGIAFTAEH
                     RRLFERVHDMVARAGRNA"
BASE COUNT          810 a         1780 c         1729 g          880 t
ORIGIN      
        1 cgatcttctt gatcttgtcg aagatctcca ccatcgcggg gctgcgcccg atgaactgct
       61 tgaagaatgc gttgcccgtc gaacccgagc gcaggtacgc gatctcgctc ctcaggcgct
      121 ggttctcgag cgcgttggaa atcgtcacgc gcagcttgtc gactgagtac ggcttggcga
      181 cgaagtcgta ggcgccgagc ttcatcgcct cgaccgcggt ctccacttcg gcgtacgcgg
      241 tcatcatgac caccggcacg gagggatcct cggccttgac catcttcagc acgtcgagcc
      301 cggatccgtc cgggagcttc atgtcgagga gcaccaagtc gggcgaagcg tcgcggaact
      361 tggcgacacc gtcggccgcc gtctcggcgt cgacggtgtc gtaaccgccc gcggcgatcg
      421 cttccgccag cgaccatcgt atggttttct cgtcgtcgat aatgaggatc gtcttcggca
      481 tttacggctc cttctgcgtg ttgtccccgc gcttacgccg gcgcgcccgc aacgggcact
      541 gcgagcgcgg gaaggcgtac cgtgaacttc gtccccttct ccagaaactc gcacgcgatg
      601 gtgcccgcgt ggtcgtccac aatttgacgc gaaatggcca ggcccagtcc cgtcccctgc
      661 gtcttggtgg agacgaacgg atcgaagagc gtggcgcgca cctcttcggc cacccccgca
      721 ccggtgtcgg cgacctcgat cgccacccac ccctcgtcgc gccgggccgc gaccgccacg
      781 cggtcgccgt gcccgcacgc ctgcaccgcg ttgaggacca gattcaagag cacctgggtc
      841 agctgcgacg ggtcggccaa gcagatcacg tccgcgtcga ccgacgtcgc caccaccacg
      901 ccccgggccg tggccgcgtc cgcggacagc gacaccacgc gcgccgccac gtcggccacc
      961 ccgcaacggg agagctgcgg cttgggcggg cgcgcgaacg cgagcaggtt cttcacgatg
     1021 ccgtcgaggc gctggatctc atcgagcatg gcgtggatgt agcgcacctt gtcgtcgtcg
     1081 cccgcgagct tctcgcccag cacctcggcg gtggtcgcga tgcccgcgag cggattgcga
     1141 atctcgtgcg ccacaccggc cgagagctgt cccagggcgc gcagccggtc gttgcggcgc
     1201 accgcttcct ccattcgctt catctccgag agatcgtcga agatggcgac cagcacggtg
     1261 ccgttgatgt tgtcgtcgtg cattttcgtc acgcgcaagc gcacgggaaa aggcgcgcgc
     1321 ccgccgtggc acgtcacctc gaggccctcc gcgtcgtcgg ccgcctccaa caggcgccgg
     1381 aacaggcccc cgtgaatcgc gtgctggaac agctcgtcca tgtgaaccgt ccccgccgaa
     1441 ccgcgtcccc gcagtcgcgg gcacatctcc gccgcccacg cgttggcgaa gcgaatcgca
     1501 ccgtcgagat cggtggtgac gacaccgctg cgcaggttgt cgagcacctt gtgattgaag
     1561 cgcgagagtt ggcgcagctc gctgatgtac gacgcgttcg cgatctcctc ggcaaagatg
     1621 gtctgcagcg tgctcaagaa gagatagtcg ccctggaagt ggtgcgcgcg gcgataagcc
     1681 ttgtccagga tcaggaaccc cagcgcgcga ccgcggtgac ggagcggaat cacggtggtg
     1741 cgccacgcgg cggtgcgctc caggcacgac gtggggcgcg agctgccgta gctttcgagg
     1801 gcgtggatct ccgcggcccg cgcgtggcgg gtccagccgt cgcgaatagt gtcgatggcg
     1861 cgccccacgt gcccttcgtc gaggtggaac acggcgcgcg cggacagcga gcgagtgccg
     1921 tcgctcaaga ggagcagccc ggcgccctcg gcttccatga agctcacgag cgccatcaga
     1981 aagcggtcga gcgcgtcgct ccagttctcg tcttcgccca ggtcgcgccc catgtgatac
     2041 agcgtcttga gctgggccgc gagcgggcgc agccgttccg gggccgcggg cgtttgggcg
     2101 ggcgcggggc cggggcgccc gtcgacccgc gggtcggggc tagccgacga catggggggc
     2161 cgacgcggcg acgaggcgac ccccgccttg ggcgcttccg gcggcgacac ccgcgtcgat
     2221 cctgcaatct tttttttcac agtgggttat ggggatccgc cgccttcgcg cggacgaaag
     2281 tggtaacacg cttaccacag ggcgggaaac gtcggcaaac aaaaaagtgg ccggctttca
     2341 gccggcagcg gggacgggga aaccgaggaa acgggtcgcg atcagaacgg gacgtggcgc
     2401 aggatgtaag actccaggcg ctcgcgatca cggtcggaga tgtcggtgaa gtagcaggcc
     2461 acctggtagt cgtcctcgcc cgcggtctcg acctcgggct cggtgcgcac cacgacaccg
     2521 tcgcacgtga cctgggacgg atccgcaccg tcctcgggca gctcgagggt gatgcgcaac
     2581 ttggtcaaga gcgggatgaa gctcgtcgag gcgaaataga ccccgttcgc gctcacgttg
     2641 aggctctcgg tgcgcgcgcg gccgccgccc agcgtgatct ccatcgccag gcgcgccggt
     2701 acgcgggccg cgcttcgccg ttcgactcgg gtcatcgctt cttctttccc aggcgcgtgg
     2761 cgtcgcctgc ttgagtgcgt ggaaaagcgg cgcggcggtc gccggagacc gccgcgcacg
     2821 tgctaacgga ttctcttctt gtgacgattc ttccgcaagc gcttcttgcg cttgtgcgtc
     2881 cgaatcttct gtcgtttgcg cttacgtccg cacggcattc gtattcctct cctcccggct
     2941 tcgctcgatc atgcgttggt gtcgttgacc cgcgccttca gctcgttgga tgccttgaag
     3001 aagggcacca acttcgcggg cacttgcaca ccgtctccgg tgcgcggatt gcgcgcttgt
     3061 cgcgcgcgtc gttccttgac cttgaagctg ccgaagccgc gcagttcgac cttgtcgccc
     3121 tccgcgagcg cacggcagat gttcgtgatc acctcgttga cgatgatgct ggtgtctttc
     3181 ttgttgagcc cggtgaccct cgagatctcc tcgaccaagt ctgctttcgt catggctctc
     3241 tcccagttag gaaactcgaa gcgcgcccgc ttcggccatc acgccaagcg atccaccgcg
     3301 cccgttacct cgaccggccg gggcgcgaca cgctcgcccg gcttcgagac gatcgtcgtg
     3361 gaagctatca cagggctgga agcactgtca aataagaatt tacagtccgt gcgagggacg
     3421 ccggcaaggc tcggagggaa aaaaaaggcg ccgccgcgcc gtgtgcttgg tctcgggaaa
     3481 caacgtcctt gagggaatgg gcccacgccg aagcaagaca cgggcgaagc agcagccttg
     3541 cccatgtacc atcaagcgac atgccacggc ccgcgcgcgc ggcccgcgcg cgtattaagc
     3601 ggcgtattca tcatcgattg gcgcgtcgcc gcgggcgtcc gtggggttgt cgcaggcgcc
     3661 tcgcggtgcg ggttccggcg cacgcagtca cgtgcggtat gagtcttcgc gcgcggggcg
     3721 gcgtgtgctg attcgcgaag tggagaggga ttcctcggcg cggcgtgttg gtcctcgccg
     3781 taaccgcgcg tcacgcgttc cggcccgcgc gcgcgaccat gtcgtgcacg cgctcgaaga
     3841 gccgccggtg ctccgcggtg aacgcgatgc cgcgacggtg catcgcggcc gtggccgtct
     3901 gcaccgcttt ctcgagctcg tagggcacca cgtcgcggcc gtggccccag gagaaactgg
     3961 gcacgaacgc gtccggcatc tcgctcgagt agaggttgca gttgaagccc accaccgtcc
     4021 cggtgttgaa cacggcgttg ataccggtct tggtgtggtc ccccatcacc aggccgagga
     4081 actggcgacc ggtatcacgc gggcggccgg cgttccacat gcgtacgctg ccgtagttgt
     4141 tcttgaggtc gctgttgttg ctcgccgccc caatgttgac ccacgagccc aggtacgtgt
     4201 gacccaggaa tccgtcgtgc tgcttgttcg tgtagtccgc gaagatggac tcgtcgactt
     4261 ccccacccac cttgcacacc ttccccaccg acgtgcccgc gagaatgcgc gccccggctt
     4321 tcacgaccga gcccgccccg atcgccgccg gccccacgat ggtggcgttg gccataacga
     4381 cgacgccttc gccgatcacg accgggccgt cgctggcgtc gatcacggtg ccggcgcgaa
     4441 tcgcggcccg gctcccgatc gcgatccgct tgggctcgac caggtgcacg ccggggtgga
     4501 tcgcggcgtc cttggcgtgc ccgcgcgccg cgcgcgcgaa atcgtcggcg atgcacgcgg
     4561 aattgtgctc gaccagctgc cacggaaacg agagcacgcg ggcctcgggc atctcgacgc
     4621 gtcgcagtcg ccgggcgtcg atcacgcgca tgagatcggg cgacaacgcc tccgcgtcgg
     4681 catccgccgc ggactgcgcg cgcgcttttt cgagctgcga acacaccgca tcgacggccg
     4741 cgtcggtgat ttgtgcgcgc agcgcgcgcg ccaagtccgc agccgcgtcg cgggaaaccc
     4801 gcgccgcgac caggcacccg ccctgaaggg ccacgctgtc gtccggcaat cgagacagga
     4861 acgcatcgcg ttcgcccgcc atgcacagcc agcgcccgtt gaccaacagc gtgtccccgg
     4921 cgaccgcgtc gttcacgggc aggccgggag ccgcgaggcg aaccacgtcc gcgaggtagt
     4981 cgcggcagat caacgagacc tggccgtgcg tgccgccggc aagccagcgc tcgcgcagcg
     5041 tcccggcccc gacgcgcagg tcgaacaccg gctgggcgag cgagagcgga aagaagttcg
     5101 cgaacttccc gtcttcgaag atgcagatgc gaccggacac gcgcgggcct acttgctcga
     5161 acgcccgcgc gatgcgcgcg ggcggttcgt cggtcgtgc
//
LOCUS       DASPGK010000065         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_484594, whole genome shotgun sequence.
ACCESSION   DASPGK010000065 DASPGK010000000
VERSION     DASPGK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484594"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1293
                     /locus_tag="VEC56_01695"
     CDS             145..1293
                     /locus_tag="VEC56_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXV12897.1"
                     /translation="MQFPLTDEQRLIQTTAREFADNEIAPVADKHNREGAFPAALVKR
                     LGELGFLGMLVPETYGGSGIGNFGLVLALEEVNRACASTGVTMSVQNSLVCAPIVHFG
                     SEDLKKKYLPKLATGAFLGAYALSEPGSGSDAAGLVASAVKDGDSFVLNGTKNFITTG
                     AEADVVIAMVRTDPAHKTRGITAFVVESGWKGFAVGKKEDKMGLRSSSTVQLVFEDMR
                     VPAANILGEVGIGFKIAMHTLDGGRIGIAAQAVGIAQACLDASIKYAEEREAFDQQIG
                     KFQAVQFKLANMATRIEAARLLVYNAARLKDAGQPHGKEAAMAKLFSSETSNEAARQA
                     VQIHGGAGYLEDFPVERYFRDARITEIYEGTSEIQRIVIARHVLKNLR"
     gene            1331..2128
                     /locus_tag="VEC56_01700"
     CDS             1331..2128
                     /locus_tag="VEC56_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXV12898.1"
                     /translation="MKIVVLVKSVPDTESKIKIGPDGASIDPQGLKFIMNPYDEFAVE
                     EGLRIKERLKDDSTVVAVSMGPERAVEVLRTALAMGADDAIHVSDPAFEGGDAQANAR
                     VMAEVIRPLAPDLILGGKQAIDYDAAQVCPAIAEYLGLPQALIVTELKISDDKKTATA
                     RRRVEGGDEIVEMSLPALVTCEKGLNEPRYASLPGIMKAKKKEIKKAGLAGLSLQPES
                     VGAAGAKAKVVRYHNLPERPPVKMIAGDAAAQAQELVRLLREEAKVI"
     gene            2181..>2693
                     /locus_tag="VEC56_01705"
     CDS             2181..>2693
                     /locus_tag="VEC56_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXV12899.1"
                     /translation="MANGILVYGEVREGSLKAITKELVAAALQLSQAGAGPVSIALVG
                     DAATVDAALPAARALPVSKVYAARHAALAAYSSQGYAMAIQAALQASGAKVLLFGATA
                     LGRDLSARAAARCDASLFTDCTELVWAGGNLRVKRPVYSGKVILELESSAGVQMASIR
                     PNIFAAAAAAG"
BASE COUNT          483 a          874 c          924 g          412 t
ORIGIN      
        1 tcccgccggg cagcgttcgt ctaaccccat gcggcaattc gcgattgaca cccaccccgt
       61 cctcccgtaa ggtcgccttc tgatccggcg cctgtgccgc cagccgtcgc ttccgttcgc
      121 tttaccatcc caacgaggga catcgtgcaa tttccgctca ccgacgagca gcgcctcatc
      181 cagacgaccg cccgcgagtt cgcggacaac gagatcgcgc ccgtcgccga taagcacaac
      241 cgcgagggcg cgtttcccgc cgccttggtc aagcgcctcg gggagcttgg ctttttgggc
      301 atgctggtgc ccgagacgta cgggggcagc gggatcggga atttcgggct cgtcctcgcc
      361 ctggaggaag tcaaccgtgc ctgcgcgtcg accggcgtta ccatgtcggt gcagaactcg
      421 ctcgtgtgcg cccccatcgt ccacttcggc agcgaagatc tcaagaagaa ataccttccc
      481 aagctcgcga cgggcgcatt cctgggcgcg tacgcattga gtgagccggg ctcgggcagc
      541 gacgccgccg ggctggtcgc ttcggcggtc aaggacggcg actcgttcgt cctgaacggg
      601 accaagaact tcatcaccac cggcgccgag gccgacgtcg tgatcgcgat ggtgcgaacc
      661 gatccggcgc acaagacgcg cggcattacc gccttcgtcg tcgagagtgg gtggaagggg
      721 ttcgccgtcg gcaagaagga agacaagatg ggactgcgct cgtcgagcac ggtgcagctg
      781 gtgttcgagg acatgcgcgt cccggccgcc aacatactgg gcgaagtcgg gatcggtttc
      841 aagatcgcga tgcacacgct cgacggcggg cgcatcggca tcgccgcgca ggcggtcggt
      901 atcgcgcagg cgtgcttgga cgcgtcgatc aagtacgccg aagagcgcga ggccttcgac
      961 cagcagatcg ggaagttcca ggcggtccag ttcaagctcg cgaacatggc gaccaggatc
     1021 gaagcggcgc ggctgctggt gtacaacgcc gcgcgcttga aggacgccgg gcagccgcac
     1081 ggcaaggagg ccgccatggc caagctcttc tcttccgaga cgagcaacga ggcggcgcgc
     1141 caggcggttc agatccacgg cggcgccgga tacctggagg actttccggt cgaacggtac
     1201 ttccgcgacg cgcgtatcac cgagatctac gaagggacga gcgagatcca gcgcatcgtc
     1261 atcgcgcgtc acgtactcaa gaatctgcgc tagggttgcg cgcggggtcg cgcggccaca
     1321 ggaggatgtc gtgaaaatcg tggtgctggt aaagagcgtg cccgacaccg agtcgaagat
     1381 caagatcggg ccggacgggg cgtcgatcga cccgcaagga ctgaagttca tcatgaaccc
     1441 ctacgacgag tttgccgtcg aagaggggct tcgcatcaag gagcgcctca aggacgatag
     1501 caccgtcgtc gccgtgtcga tgggccccga gcgggccgtc gaggtgctgc gcaccgcact
     1561 cgcgatgggg gccgacgacg ccatccatgt gagcgaccct gccttcgagg gcggcgacgc
     1621 gcaggccaac gcgcgcgtga tggcggaagt gatccgaccg cttgcaccgg acctgatcct
     1681 gggcggcaag caggccatcg actacgacgc cgcgcaggtg tgccccgcca tcgccgaata
     1741 cctcggcctg ccgcaggcgc tgatcgttac cgaactcaag atctccgacg acaagaagac
     1801 cgccaccgcg cgccggcgcg tggaaggcgg agacgagatc gtggaaatgt cgctgccggc
     1861 gttggtcacg tgcgagaagg ggctcaacga gccgcgctac gcgtcgttgc ccggcatcat
     1921 gaaggcgaag aagaaagaga tcaagaaggc cgggctggcc gggctgtcgt tgcaaccgga
     1981 gtcggtcggc gcggcgggtg cgaaggccaa ggtcgttcgc taccacaacc ttcccgagcg
     2041 cccgccggtc aagatgatcg cgggtgacgc ggcggcgcag gcccaggagc tcgtgcgcct
     2101 cctgcgcgaa gaagcgaagg tcatctgacc accgaccacg accgctgaaa ggagccgggg
     2161 cgctggcgtc ccacgtcgac atggcaaacg gaattctggt ctacggagaa gtgcgggagg
     2221 ggtcgctcaa ggcgatcacc aaggaactgg tcgcggccgc gttgcagctg tcgcaagcgg
     2281 gcgccggccc ggtttcgatc gcgttggtgg gggatgctgc caccgtggac gccgcgctcc
     2341 ccgcggcgcg cgccctcccc gtaagcaagg tttacgcggc gcgccacgcc gcactcgcgg
     2401 catactcgtc gcagggctac gcgatggcga tccaggccgc gctccaggcc agcggcgcca
     2461 aggtcttgct gttcggcgcg accgcgctcg gtcgcgacct ctccgcacgc gccgccgcgc
     2521 gctgcgacgc gtcgctgttc accgactgca cggagttggt gtgggcgggc ggaaatctgc
     2581 gtgtcaagcg cccggtctac tcgggcaagg tgatcctcga actggagtcg agcgcgggcg
     2641 tgcaaatggc gtcgattcgt cccaacatct tcgccgctgc cgccgccgcc ggc
//
LOCUS       DASPGK010000066         9556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_485200, whole genome shotgun sequence.
ACCESSION   DASPGK010000066 DASPGK010000000
VERSION     DASPGK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485200"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VEC56_01710"
     CDS             complement(<1..218)
                     /locus_tag="VEC56_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXV12900.1"
                     /translation="MSTQRIEEANVVTRKLPNGLSVILKQDRTSPIVAVNVWFGVGSV
                     HESEEMNGLAHFQEHMVFKGTEKYKVGD"
     gene            complement(757..2334)
                     /locus_tag="VEC56_01715"
     CDS             complement(757..2334)
                     /locus_tag="VEC56_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV12901.1"
                     /translation="MSANILLVDDDPKVLEILSVSLERRGHRVSTADTGSSAIEQTSA
                     SPPELIILDLVLSDMNGVRVLEHVRDKHGDDPPVLFLSAETDVDARLAALERGADDFL
                     IKPVSLRELHSKVDGALRRSTRMRALGQSKRTLEAEIAQRRAHASHVSKEFKRHLLSM
                     RTLLTMSHDLNRALQSEDLIKMASLTLLGELKVSSMALFALEHESDKEFPLLGVRGFD
                     AKRFDGLALDRTSAFVSMLVDGGRPQQIARNQDQKWVKKLPDLRLAVFEYAAPIAFKG
                     EIKGVVFTGPKLSGEAYSEYDLDIMMFIANSIALGMENSHLVTQLQTTYVSTLRSLIS
                     IIEAKDPYTKGHTERVASYAMALANRLSFDEETLRTILFGALLHDIGKMGVLDEIIQK
                     PGPLTEDEWELMRAHPVVGAQIVEKMEFLTGTIDIVRHHHESWNGKGYPDGLRGEEIP
                     LLARLVTVADSFDAMTTDRPYRKALSLDEAVRRLEEASGVQFDAELVKVFVKYVREKG
                     RDLTVVAAAETRGVADL"
     gene            complement(2356..4116)
                     /locus_tag="VEC56_01720"
     CDS             complement(2356..4116)
                     /locus_tag="VEC56_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV12902.1"
                     /translation="MPPKTTIEKLSSSSPDVTIFKITGTLGFHEKNVLERLFNECARR
                     GLARVLFEVSELESLGGGCARIIREEAMRGRLAIGLAGATRTVLKFLQNDDVSRVVLA
                     ETIDQAIPLVQAKALAAPATPSAEVDSDEALLSKESLEEILRLGDVSADEEDADHDDE
                     EDAPHGASKKTRGKGGDAAKPARADAKSDAKPAAPPKEAPKAASRAAAPASPPPDLDD
                     SKQLQKRIVQYNTLFSINSDFYRFQDRKNLLDAFLLTTIAQVGVESAVFLESNKGYFV
                     PVAMKGIEAGELRGFALSAAQLCLPKLLALAGVILVEKSPFGDDVKAPLLALGCTYVA
                     PFVVHGEVRGVVLLGKPLKKELDTTTMDFMKILINQAAIAYESMARFEEENERTLGIV
                     QTLISLIEENTLARGNTNLISHYVFAVAKRMHYPDEHVRDLMYGTVLRDIGMIKVSDL
                     IVRSPRELMPEEWEIIKRHPSDGAKMLKDMKFSTHATDVVQNHHERFNGEGYPRGAQG
                     QDVPLGARIVSVVESYAAMLQVRPTRPALSREEALNTLKENWGMRYDPEVIRCFVEIV
                     DEEIRSGVDSSDQRFALFSV"
     gene            complement(4168..4392)
                     /locus_tag="VEC56_01725"
     CDS             complement(4168..4392)
                     /locus_tag="VEC56_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV12903.1"
                     /translation="MSKWRDTDYSSIVIGGAEDQDVVMTSVEAAEYLKMHVKTVCRLA
                     KERKIPAKKVGSEWRFLKSVLDKWLAQEVV"
     gene            complement(4599..6437)
                     /locus_tag="VEC56_01730"
     CDS             complement(4599..6437)
                     /locus_tag="VEC56_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV12904.1"
                     /translation="MSVRMLAGTMLVVDLADWTRLLGSVESLLNVIGQRRLAWDERLA
                     QVIFELIEKEELLASCHERDGEEGGRNAVSTQEVEALIAEVDALREECERAESVPMPA
                     NEVAVEVERMGRVATELGAACERVLAAVGSQEWQSRGADASAVETLRRSLGTIEFYAQ
                     AMDRIVASVEEDVTAPKCDIVPIETALGDFASELVRETGRELEIRLHADGLEIEPALM
                     PAAYWILHAMVADVFERCDEPKIEIDINMSDRNGAIHCRLKDNGENFLSDSRLDHEDQ
                     LAFYPGLRHVMRTLKEHHGTLSVEPGGGKEARFGFTLPSTTVAEPLLVWERAGHTFAA
                     RASQVVDVVAISANLRKAGSYGEHIMIDGRRVPLLKLDVLFPDAPTGNSHVVVLGALE
                     KHVAFYAPGEGAPKLGVVLGDSTPVWRGIPHDVVRVDGRRVPLMDAADILRSYLSITG
                     TLNSDAMSAAEPDSGGRDLVHEPEEYDAPVADAAADDPRADDPEVLVIEQSESTRNAL
                     TDILARQRVRASFAGDVEEALGLIRSSEPRVIISEFRIPTMAAKKIVDSLRAQGRAIP
                     VLVTTSQSGKTADLLVEKLGASGYLSKPLSAAQVASQLSTFLGVVH"
     gene            complement(6583..8517)
                     /locus_tag="VEC56_01735"
     CDS             complement(6583..8517)
                     /locus_tag="VEC56_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12905.1"
                     /translation="MDRKKSPVLTSPGKVGFFVALLLVVTALGVSYFITGTFGLQWNW
                     INLDGVSPAEWKFNLGAFVDEMVPLIALVALLAFASYVLVTGAVRRYQSSVSSGAEFR
                     ELLRSFKSMDDFEDAGRLEELKQHPELREFVMGFKNRMAARERQLEEREKRHKESGSS
                     RASSSASSSDRGNGNLASESAILLTAIVESKNGFSDGLSLTIPELKQIERAVRERLNG
                     QRVVQDDSAKRELDNLKASVDTTLAAVRSAVASARRDASSCMTGAREIEAGLASLQQA
                     IDALAVPAAAATGVATAAKRVDAVADALASLGEETKRIAIAAALSASGGAEGDAIKVA
                     EEVRTIATRFNAVAQQWKETSPAIRSAIDTIASNAVGAEKRRTAALKALEGVVAKTRL
                     WGERMVALAEAVNGLDRATGGKSKPAAAPAAPAAVAPTQEDWGNLAASLEEDVQAPEP
                     RASARAAAEPEPSVEEDFVPQRAANVFEDTPDDTPFVDIPGFEKDRNLFTDDAGRKTD
                     HHEHETDPRFVVEREAGGDWDLSRATKAAETAGEVDATHASQRPNAPAPAPSAAEDDG
                     FLTGPSSNETPAASKQASAAKPAPAAKPAPAAKQAKPSRRIKVDDIAVADAPARVDAD
                     ADAVDLYAIGAVDFVAGVHA"
     gene            complement(8765..>9556)
                     /locus_tag="VEC56_01740"
     CDS             complement(8765..>9556)
                     /locus_tag="VEC56_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXV12906.1"
                     /translation="YLQLRPQFGRTKENVRAAKSMLVGVTDATVVLPELFNTGYVFRN
                     MEEVRDLAEPATGATVVELRKIAIAQRLNLVFGFLEAKNRKYYNSSALITSKGATHVY
                     QKAHLFDREKLFFQPGNGTFKAYPVEGAKIGMMICFDWFFPEVTRSLALDGAQVICQP
                     ANLVLPWCQDAMRTRSIENRVFSVTANRIGMEKRGNISLTFTGKSQVVSPKGEVLASA
                     SERSETLKVVEIDAAEALDKHVTPNNDLFRDRRPTLYKALLRKVV"
BASE COUNT         1488 a         3149 c         3175 g         1744 t
ORIGIN      
        1 atgtcgccga ccttgtactt ctcggtcccc ttgaacacca tgtgttcttg gaagtgcgcg
       61 agaccgttca tctcctcgct ttcgtggacc gaacccacgc cgaaccagac gttgaccgcc
      121 acgatgggcg aggtccggtc ctgcttgagg atgacggaga ggccgttggg gagtttccgc
      181 gtgaccacgt tggcttcttc gatgcgttgt gtgctcatag taggccgaat ataccgaaag
      241 caagcggtgg gcccaaaaga caaaaagagg gagcgaagga ctcgatcgtc gtgcgctcct
      301 tggcaccggt ggcagccccg agtatggggt tccgtgagca cacccacctc gagtggctgg
      361 ctgcacacgc tcggatccgg ccctccaccg gcgctccggc tcgcccgacg atcgaatcca
      421 cggtcgaaag acggctcctc ccaaccgccc tcctcccggc tccctctttt ctaatcacgc
      481 acccgtcgcg aacgcggcga ccttcacccc tgcactaccg ccatcaccac acgccgcctt
      541 gcgcgctccg ggtcgatcat gagtgacggg gcctagggca agcgaagtgc cattcgggcg
      601 agagcgcgcc tagaagaaat cgtcaatcgt aagacgcgcg gcgtggtaac gagttgcgtg
      661 gtagcgggtg atcggcagcc gcggtttctc ccgcgctgcg cgcgcacgcg agctgtcgcg
      721 cgggttcaca attcggcggg aagatgacgg cggtgtttac aggtccgcga cgccgcgcgt
      781 ctccgcggcg gccacgaccg tgaggtcgcg gcccttctcg cgcacgtact tgacgaatac
      841 cttgaccagc tcggcgtcga actggacgcc ggaggcctct tcgagacggc gcacggcttc
      901 gtcgagcgag agcgccttgc ggtacggacg gtccgtggtc atggcgtcga agctgtcggc
      961 caccgtcacc agacgggcca ggagcgggat ctcctcgccg cgcagcccgt cggggtagcc
     1021 cttgccgttc cagctttcgt ggtggtgccg cacgatgtcg atggtgccgg tcaggaactc
     1081 catcttctcg acgatctgcg cccccaccac cgggtgcgcg cgcatcagct cccactcgtc
     1141 ctcggtcagc ggccccggct tctggatgat ctcgtcgagc acgcccatct tgccgatgtc
     1201 gtgcaggagc gcgccgaaca ggatggtgcg cagcgtctct tcgtcgaacg agagccggtt
     1261 ggccagcgcc atcgcgtacg acgccacgcg ctcggtgtgc cccttggtgt acgggtcctt
     1321 ggcctcgatg atcgagatca gcgagcgcag cgtggacacg taggtcgtct gcagttgtgt
     1381 caccaagtgc gaattctcca tccccaacgc gatggagttc gcgatgaaca tcataatgtc
     1441 gaggtcgtac tcgctgtacg cctcgcccga gagcttgggg ccggtgaata cgacgccctt
     1501 gatctcgccc ttgaacgcga tcggcgccgc gtattcgaac accgccaggc gcaagtcggg
     1561 cagcttcttg acccattttt ggtcctgatt gcgcgcgatc tgctgcggcc tcccgccgtc
     1621 gaccagcatc gaaacgaacg ccgaagtgcg gtcgagtgcg aggccgtcga agcgcttggc
     1681 gtcgaaaccc cgcacgccca ggagcgggaa ctccttgtcg ctctcgtgct cgagcgcgaa
     1741 gagcgccatg ctcgacacct tgagctctcc caggagcgtc agcgacgcca tcttgatgag
     1801 gtcttcggac tgcagcgcgc gattcaggtc gtgcgacatg gtgagcagcg tgcgcatcga
     1861 taggaggtgg cgcttgaact ccttcgacac gtgcgacgcg tgtgcgcgcc gctgggcgat
     1921 ctcggcctcc agcgtgcgct tgctttggcc gagggcgcgc atgcgggtgc tgcggcgcag
     1981 ggcgccgtcc accttggagt ggagttcgcg caacgacacc ggcttgatca ggaagtcatc
     2041 ggcgccgcgc tcgagcgcgg ccaggcgcgc atcgacgtcg gtctcggcgg agaggaacag
     2101 cacgggcggg tcgtcgccat gcttgtcgcg aacgtgttcg agcacgcgca cgccgttcat
     2161 atcggatagg accaggtcga gaatgatgag ctcgggcgga gacgccgagg tctgctcgat
     2221 ggcgctcgag ccggtgtcgg cagtggagac gcggtggccg cggcgctcca gcgagaccga
     2281 caggatctcc agcaccttgg ggtcgtcgtc gaccagcagg atattggcac tcattgattt
     2341 ctccccgtcg tgccgctata cactgaacag cgcgaagcgc tggtcgctcg agtcgacgcc
     2401 cgagcggatc tcttcgtcga cgatctcgac gaagcagcga atcacctcgg ggtcgtagcg
     2461 catgccccag ttctccttga gcgtgttgag cgcctcctcg cgcgagagcg cggggcgcgt
     2521 cgggcggact tgcagcatgg ccgcatagct ctccaccacc gagacgatgc gcgcgcccag
     2581 cgggacgtcc tgcccctgcg cgccgcgcgg atagccctcg ccgttgaagc gctcgtggtg
     2641 gttctggacc acgtcggtcg cgtgcgtcga gaacttcatg tccttgagca ttttggcacc
     2701 gtcgctcggg tggcgcttga tgatctccca ctcctccggc atcagctcgc gggggctgcg
     2761 cacgatcagg tcgctcacct tgatcatgcc gatgtcgcgc agcacggtgc catacatcag
     2821 gtcgcgcacg tgctcgtccg ggtagtgcat gcgcttggcg accgcgaaca cgtagtgcga
     2881 aattagattc gtattgccgc gcgcgagcgt gttctcctcg atcagcgaga tcagcgtctg
     2941 cacgatgccc agcgtgcgct cgttctcttc ctcgaagcgc gccatgctct cgtacgcgat
     3001 ggctgcctgg ttgatgagga tcttcatgaa gtccatggtg gtggtatcga gctccttctt
     3061 gagcggcttg cccaggagca ccaccccgcg cacctcgccg tggaccacga agggcgccac
     3121 gtaggtgcaa ccgagggcca acagcggcgc cttgacgtcg tcgccgaagg gcgacttctc
     3181 gaccaggatc acgcccgcca gcgcgagcag cttgggcagg cacagctgtg ccgccgaaag
     3241 cgcgaagccg cgcagctcgc cggcttcgat gcccttcatg gccacgggaa cgaagtagcc
     3301 cttgttgctc tcgaggaaca cggcgctctc cacgccgacc tgcgcaatag tggtcaacaa
     3361 gaacgcgtcg agcaggttct tgcgatcctg gaagcgatag aagtccgagt tgatcgagaa
     3421 tagcgtgttg tactggacga tgcgcttctg gagctgcttg gaatcgtcga ggtcgggagg
     3481 aggggatgcg ggagccgccg cccggctggc ggccttgggc gcttccttcg gcggcgccgc
     3541 cggtttcgcg tcgctcttcg cgtcggcgcg cgccggcttg gccgcatcgc cgcccttgcc
     3601 gcgggtcttc ttggacgcgc cgtgcggcgc gtcttcctcg tcgtcgtggt ccgcgtcctc
     3661 ctcgtcggcg gacacgtcgc ccaggcgcag aatctcctcc agcgactcct tgctgagaag
     3721 cgcttcgtcg gaatccacct cggcgctggg ggtcgcgggc gcggcgagcg ccttcgcttg
     3781 gacgagcgga atcgcctggt cgatggtttc cgccaagacc acgcgggaga cgtcgtcgtt
     3841 ctgcagaaac ttgagcaccg tcctcgtcgc gcccgcgagc ccgatggcga gtcgtccgcg
     3901 catcgcctct tcgcggatga tgcgcgcgca cccgccgccc aaggactcga gctccgagac
     3961 ctcgaacaaa acgcgggcaa gcccgcgcct tgcgcactcg ttgaagagtc gctctaggac
     4021 gttcttttcg tggaagccaa gggtgcccgt gatcttgaaa atcgtgacgt ccggggagga
     4081 cgacgaaagc ttctcgatcg tggtcttcgg cggcatggct gctcccggcg atgcgacgcc
     4141 gcggggagaa agagggtgat caggccgtta aacgacctct tgcgccagcc acttatcaag
     4201 cacgctcttc aggaacctcc actcgctgcc aaccttcttc gccgggatct tacgttcctt
     4261 agcgagacgg cagacagttt tcacgtgcat cttgagatac tcggcggctt cgaccgacgt
     4321 catcacaacg tcctggtcct cggcgccgcc gatcacgatg ctcgagtagt cggtgtctct
     4381 ccacttgctc atcttgcgcc catccttctt gttgacggtg ctgcctgagt tcccacccaa
     4441 gtagaccgat tcaaagcacg aagcgtacca aagacatccg ctttattttt caagcggtta
     4501 ggtcgcttcg accccatttt agggcgcgcg gacgggcagt ttgcaccgtg cggcgccgtc
     4561 aaacgcaaac ccgataaacg acgcggtacc cgcggcgtct agtggaccac tccgaggaac
     4621 gtcgacagct gcgacgccac ctgggcggcc gacaggggct tgctcaagta tcccgacgcc
     4681 cccagtttct ccaccaggag gtcggccgtc ttgcccgact gcgaggtcgt gaccagcacc
     4741 gggatggcgc ggccctgcgc gcgcagcgag tcgacgatct tcttcgcggc catggtcggg
     4801 atgcggaact cggagatgat cacgcgcggc tcgctcgagc gaatcagccc cagcgcttcc
     4861 tcgacgtccc ccgcgaacga cgcgcgcacc cgctggcgcg cgaggatgtc ggtgagcgcg
     4921 ttacgcgtcg attcgctctg ctcgatgacc agcacctcgg ggtcgtccgc ccgcgggtcg
     4981 tcggcggccg cgtcggccac gggcgcgtcg tactcctcgg gttcgtgcac gaggtcgcgg
     5041 ccgccgctat cgggttcggc ggccgacatg gcgtcgctgt tgagcgtgcc cgtgatcgag
     5101 agatagctgc gaagaatgtc ggcggcgtcc atcaaaggaa cgcggcggcc gtcgacgcgc
     5161 accacgtcgt gcgggatgcc gcgccacacc ggagtcgagt cgcccagcac cacacccagc
     5221 ttgggagcgc cttcgcccgg cgcgtagaac gccacgtgct tttccagggc gcccagcacc
     5281 accacgtgcg agttaccggt gggggcatcg ggaaacaaga cgtcgagttt gaggagcgga
     5341 acgcggcggc cgtcgatcat tatatgctcg ccgtacgagc cggccttgcg caggttggct
     5401 gagatggcga ccacgtcgac cacttgcgac gctcgcgccg cgaaggtgtg gccggctcgc
     5461 tcccacacca agagcggttc ggcgaccgtc gtgctcggga gcgtgaaccc gaagcgcgct
     5521 tccttgccgc cgcccggctc gaccgacagc gtcccgtggt gctccttgag cgtgcgcatg
     5581 acgtggcgaa ggccggggta gaaggccagc tggtcttcgt ggtcgaggcg cgagtcggac
     5641 aggaagttct cgccgttgtc cttcaggcgg cagtggatcg cgccgttgcg gtccgacatg
     5701 ttgatgtcga tctcgatctt gggttcgtcg cagcgctcga acacatcggc caccatcgcg
     5761 tgcaggatcc agtacgcggc cggcatgagc gcgggctcga tctcgaggcc gtcggcgtgg
     5821 aggcgaatct cgagctcgcg gccggtctcg cgcacgagct cgctcgcgaa gtcccccagg
     5881 gccgtctcga tggggacgat gtcgcacttg ggtgcggtca cgtcttcttc caccgaggcc
     5941 acgatgcgat ccatcgcttg cgcgtagaac tcgatggtgc cgagcgagcg gcgcagggtt
     6001 tcgacggcac tggcgtcggc tccgcgcgac tgccactctt gggatccgac ggcggccagc
     6061 acgcgctcac aagcggcgcc cagctccgtg gccacgcgtc ccatccgctc gacttcgacc
     6121 gcgacttcgt tcgccggcat cggaaccgac tcggcgcgct cgcactcttc gcgcagcgcg
     6181 tccacctcgg cgatcagcgc ttccacttcc tgggtcgaga cggcgttgcg cccgccttcc
     6241 tcgccgtcgc gctcgtggca cgacgccagc agctcctctt tctcgatgag ctcgaagatg
     6301 acctgcgcga gccgctcgtc ccaggcgagg cggcgctggc cgatgacgtt gagcagcgat
     6361 tcgaccgatc ccaggaggcg cgtccagtcg gcgaggtcga ccacgagcat ggtccccgcg
     6421 agcatgcgca cgctcacctt gcagcgctcc agcgtgggct cggggacgag actaccggcc
     6481 gtacaagcga gaagctcgca gagcgtttcg cactgcgagc gaacttgacg ggcgaagagt
     6541 gcgcggctgt ccattgggtc gtgcggtctc cgggacgcgt gcttaggcgt gcacgcctgc
     6601 tacaaagtcg acggctccga tggcgtacag atccacggca tccgcgtccg cgtcgacgcg
     6661 cgcgggggcg tccgccaccg cgatgtcgtc gaccttgatg cggcgcgacg gcttggcctg
     6721 cttggccgcc ggcgcgggct tggccgcggg agccggcttg gcggccgatg cttgcttgga
     6781 cgcggccggc gtttcgttgg agctcggacc ggtgaggaag ccgtcgtctt cggccgcact
     6841 cggcgcgggc gcgggagcgt tcggacgctg ggacgcgtgg gtcgcgtcga cctcgcccgc
     6901 ggtctcggcc gccttcgtgg cgcgcgacag atcccaatcg ccgccggctt cgcgctcgac
     6961 cacgaagcgc ggatcggtct cgtgctcgtg gtggtcggtc ttgcggcccg cgtcgtcggt
     7021 gaacaggttg cggtccttct cgaaaccggg aatgtcgacg aacggggtgt cgtcgggagt
     7081 gtcttcgaac acgttcgcgg cgcgctgggg aacgaagtcc tcctcgaccg acggctcggg
     7141 ttcggccgcg gcacgagcgc tggcgcgcgg ctccggcgcc tgcacgtctt cctcgagact
     7201 cgcggcgaga ttgccccagt cttcctgggt cggagcgacg gccgcgggtg cggcgggcgc
     7261 ggccgccggc ttgctctttc cgcccgtcgc gcgatcgagc ccgttgaccg cttccgccag
     7321 cgccaccatg cgttctcccc acaggcgcgt cttggcgacg acgccctcga gggccttcaa
     7381 tgcggccgtg cggcgcttct cggctcccac cgcgttgctc gcgatggtgt cgatggcgct
     7441 tcgaatggcc gggctcgtct ctttccactg ctgggccacg gcgttgaagc gcgtcgcgat
     7501 ggtccgcact tcctcggcca ccttgatggc gtcgccctcg gccccgccac tcgcggaaag
     7561 tgccgcggcg atcgcgatgc gcttggtctc ttcgccgagg ctcgcgagcg cgtccgctac
     7621 cgcgtccacg cgcttggcgg cggtcgcgac gccggtcgcc gccgccgcgg gcaccgcgag
     7681 cgcgtcgatg gcttgctgga gcgacgcgag cccggcctcg atctcgcgcg cgcccgtcat
     7741 gcacgaactc gcgtcacggc gcgccgacgc caccgcgcta cgcaccgcgg ccagcgtggt
     7801 gtccacactc gccttgaggt tgtccaactc gcgcttggcg gagtcgtcct gcacgacgcg
     7861 ctggccgttc agccgctcgc gcaccgcgcg ttcgatctgc ttgagctccg gaatggtaag
     7921 cgataggccg tcgctgaatc cgttcttgga ttccacgatc gcggtcaaga ggatcgcgct
     7981 ctcggaagct aggttgccgt tgccgcggtc cgacgacgac gcggacgacg aggcgcgcga
     8041 cgagccgctt tccttgtggc gcttctcccg ctcttcgagc tggcgttcgc gcgcggccat
     8101 gcggttcttg aaacccatca cgaactcgcg cagctcgggg tgctgcttga gctcctcgag
     8161 ccggcccgcg tcttcgaagt cgtccatgct cttgaacgag cgcaggagtt cgcggaattc
     8221 ggcgccgctg ctgacggagg actggtaacg acggacggcc ccggtgacga gcacgtagga
     8281 cgcgaacgcc agcagcgcga ccaacgcgat cagcggaacc atctcgtcga cgaaggcgcc
     8341 gaggttgaac ttccactccg ccggagacac gccgtccagg ttgatccagt tccactgcaa
     8401 cccgaaggtc ccggtgatga agtagctgac gccgagtgcg gtcacgacca ggagaagggc
     8461 gacgaaaaag ccgaccttgc cgggcgacgt cagtaccggt gacttcttgc gatccacgtt
     8521 gagactcctt cgttatggcg cgccggcggt tccggtcggt gccgggcagg ttgggtcgcg
     8581 cgagtgcact gtgctcgcgc cgatcacgca aggaatatgc cagcgaagtg cgccgtggcg
     8641 gacccggcga cgagcgctaa cgcattattt ggaaaaggga tagaggggcc gcgaaggacc
     8701 gcgagagggc ccgtccacgt gcagtttgca cggacgggga caccctcgcg ttgctttttc
     8761 gggactaaac gaccttgcga aggagagcct tgtacaaggt ggggcgtcgg tcgcgaaaca
     8821 ggtcgttgtt cggcgtcacg tgcttgtcga gcgcttccgc cgcgtcgatc tcgaccacct
     8881 tgagcgtttc gctgcgttcg ctcgcactcg cgagcacttc gcccttgggc gaaaccacct
     8941 ggctcttgcc cgtgaacgtc agcgagatgt tgccgcgctt ctccatgccg atgcgattgg
     9001 ccgtcaccga gaacactcgg ttctcgatcg agcgcgtgcg catcgcgtcc tggcaccagg
     9061 gcagcacgag attggccggc tgacaaatca cctgggcgcc gtcgagcgcg aggcttcgcg
     9121 tcacttccgg gaagaaccaa tcgaagcaga tcatcatgcc gatcttcgcg ccttcgacgg
     9181 gataggcctt gaacgtgccg ttgccgggtt ggaagaacag cttctcgcgg tcgaagaggt
     9241 gcgccttctg atacacgtgc gtggcaccct tggacgtaat caaggccgag gagttgtaat
     9301 acttgcggtt cttcgcttcc aggaatccga agacgaggtt gagtcgctgc gcgatcgcga
     9361 tcttgcgcag ctcgacgacg gtggcgccgg tcgcgggctc ggcgaggtca cgcacctctt
     9421 ccatgttgcg gaagacgtag ccggtgttga agagctcggg caggacgacg gtggcgtcgg
     9481 tgaccccgac caacatggac ttggcggcgc ggacgttttc cttggtgcgg ccgaattgcg
     9541 ggcgcagctg aaggta
//
LOCUS       DASPGK010000067         7750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1656346, whole genome shotgun sequence.
ACCESSION   DASPGK010000067 DASPGK010000000
VERSION     DASPGK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656346"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VEC56_01745"
     CDS             complement(<1..1604)
                     /locus_tag="VEC56_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12907.1"
                     /translation="MLAFGVAVPAVLMLPLQVLTWIHLMLVPGMALYLLVERGRPHAV
                     DALQAALVLSPVTLVGVGLLGLVTHTATLSIAAWVAVTALAALASCVRRAGPVITPPR
                     RELSLFLVLLGATVVLVAILPLTREWWRIRSDAWFHAAVVLQMRDFGVPPQDPYFAGI
                     KLQYMWAYHAVVLYLGDLLRVDYFRVMAFVNWHALTLLAVAAYRLATIFRTGTAPRIA
                     AVATVLFAFNGAFWLFLPIKAAKAFIGDVRGFDEIARMFALTPLDLTSAYAFLDIYRN
                     PEFFLDKFMVATAFSMALSFMLAALSSACAFLSASRPASLIVLSASTAGALLFHMYVG
                     LIALAAIMGAAAAILLFRSAIRGYTLRSALYPALCAAASAAVTAPFFYIVTRDRVGNG
                     PAALLNISLDRALSIAIPCALVLVLAWREGRIRNDRSPPARFLVFSALATLIACLVVS
                     LPGANDLDKPAFPVFLTVAVVAGFAIADHCESRRGPWRRAAPVLWVALFAVPCNAVAF
                     AGCFATPDRIEVTSEEHALGRWVRQAT"
     gene            complement(1819..2259)
                     /locus_tag="VEC56_01750"
     CDS             complement(1819..2259)
                     /locus_tag="VEC56_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HXV12908.1"
                     /translation="MIVATTRLEIATDEGASLSDVTSDVNSFVRASGIANGLCVLSAP
                     SSEACLTLSGELDEDVDDLLRLAWSHLAGRPGDAPDGGDEPGERPDRVDAEGAGYSPA
                     GVLAECISLPVRDGAIGLGTWEAVVLLDARGPALRPIDVTVMGS"
     gene            2457..3791
                     /locus_tag="VEC56_01755"
     CDS             2457..3791
                     /locus_tag="VEC56_01755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HXV12909.1"
                     /translation="MTKKSFRLPFGRPEDATGDELSVLREENRQLKRAVEELSILNEL
                     SRAIGASVDSEDIIRKIVDRSMRAVQAEQTVVTLVDRNANQPMKTLVRAMTSSAEHPH
                     YHVNENLLGWMHLYKKPLLTNDPTTDERFRGLKWDQTIKSILCVPLMVKSELIGVITS
                     YNKKGQGGFNEEDQRLLAIIAGQSAQIIENARLYEEEKALVSMKEQVKLAAQIQQDLL
                     PKAPPRLEGYDIAACSMAAQMVGGDYYDFVRVRDGRWAVCLGDVSGKGLPASLLMATL
                     QATLRGQTALEHAVRETISRSNRLIYQSTDPEKFATLFFAVLDTNGGGLAFCNAGHEY
                     PMLFKKGGGPPARLITGGMALGVLEEFPYEEDAARMDAGDTLIVYSDGITDAVNEFDQ
                     PFGEERLQKCVTDHAGERADEIMNRVVAAVKAHEGVAPRIDDLTLIVVKRVS"
     gene            3881..6766
                     /locus_tag="VEC56_01760"
     CDS             3881..6766
                     /locus_tag="VEC56_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV12910.1"
                     /translation="MIGQRVQHYQITEKLGEGGMGVVYRAQDTRLNRAVALKFLHPAI
                     VASPDDQQRLVQEARAAAALSHPNICTVYEINEHDGRTFIAMQFVEGSTLRDRVLSGR
                     IETVDALRYLIQIADGLKQAHEQGIVHRDMKSSNIMITPSGRAVIMDFGLARPAGTGR
                     TDERFSSRGTSAYMSPEQARGEMVDQRTDIWSLGVVLYEMLAGRLPFRGDYEQAVVYS
                     ILNEPHRPIAEFRAGLPAAVTKVVETCLAKNPADRYQSLDALIHAVRGALDDMSGLRR
                     RDGERWGRARLAGVAAAAVLTVAAGFVVFDFLRGGRAGGEERVPIAVIDFNNETDEAA
                     LDGLSGMLITALEQSRRLSVMTRSRMFDIAKTIGHGDAARIDEALGQRICEAAGVDAL
                     VIPTIRRFGDLYSIDLKVMDTRRHNYVFTTKEEGRGLESIPNMVDAIARDIRIDLRET
                     TESVARTAPLADVTTTNLGAYQAYFEGEELLDRLQFTPAGKAFERAIARDSTFALAYY
                     RLAYTEWWSRQNAKEAKQHIDYAIANLDRIPQKERYLVRALKVALDDGFEAQLPVLKE
                     MRTLYPDDKEMLFGLGDAEFHSNEFDSAEVHFRAALAIDPEMERALQHLAWTLLRLER
                     YEETLVIAERWTKAAGSSEAYEFLAIAHMRLGRPDTALRILDETIARDPENPKLVVRK
                     SLVYFTTHRPNESLAALDQAVRLGSKDPVVQLEIGQMRSMVLYPYLGRYRESARLLDA
                     ARAVSLTTFGDSTAAVNLAVAERALAYWAHQDARRAIADLEALQTVRAKLLTDNYWLT
                     LSAFHMIAGDTAEAGAIQRAHSKNLTEEQRASIGVFKAATSGDCEGAEALMRSAAAGK
                     GVPGSSEDDRLYVIGRCQLAGGAYDGAIASLRRILDRPVTFADSGPMIPVAWFYLGEA
                     YERKGDVAKAAAAYDRVLEIWKDGDDDLYCRREARIRLDRLAGTRSM"
     gene            6766..>7750
                     /locus_tag="VEC56_01765"
     CDS             6766..>7750
                     /locus_tag="VEC56_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV12911.1"
                     /translation="MVAFRSSHVVLTITFAVTLACNRSPSQPADLILNNGIVHTFAGS
                     ASSNARATAIAVRGERIVYVGDDAGAAAWRGNETRTVDLAGKTVLPGLVDAHGHLANL
                     GRSLEEVQLVGSTSREDVLRRVRQTQLQIPAGEWIHGRGWDQNDWEQTSFPTWRDLAG
                     TEANPVYLERVDGHAVWLNRTALDACGITRATPDPRGGRIERDANGEPTGVLVDEAEK
                     LLVGNVPEPTDDDLDRRLARAIAECNRLGLTGAHDAGTDPNVLASLRRLGERGALTIN
                     VYCMLDSDAPALVRSFFASGPSIEYEGRLVVRSVKLRADGALGSRGAALLRP"
BASE COUNT         1461 a         2476 c         2609 g         1204 t
ORIGIN      
        1 gtggcctgcc gcacccaccg gccaagagcg tgttcctcgc tcgtgacttc gatgcggtcc
       61 ggtgtcgcga agcagcctgc gaacgcgact gcattacaag ggacggcaaa gagggcgacc
      121 cagagcacgg gcgcggcgcg gcgccagggt cccctgcgcg actcgcagtg gtccgcgatc
      181 gcaaagcccg ccaccaccgc gaccgtgagg aacaccggga aggccggctt gtccaggtcg
      241 ttggctccgg gcaggctcac cacgaggcac gcgatgagag tggcgagcgc gctgaaaacg
      301 aggaatcgcg ccggcgggga acggtcattt cggatcctgc cctcgcgcca ggcgagcacc
      361 aggaccagcg cacagggaat ggcgatggac aaggcccggt ccagagagat gttcagcagg
      421 gcggctgggc cgttgcccac tcggtctcgc gtcacgatgt agaaaaacgg cgccgtcacc
      481 gccgcgctcg ccgcggcgca caacgcgggg tagagagcgg agcgcagcgt gtaaccgcga
      541 atcgcggagc ggaacagcag aatggctgcg gcggcgccca tgatggccgc cagcgcgatc
      601 aaccccacat acatatgaaa gagcagcgct cccgcggtgg aagcggacag gacgatcagc
      661 gaagccgggc gggatgcgga gagaaacgcg cacgccgacg acagggcggc gagcatgaag
      721 gacagcgcca tgctgaacgc cgtcgccacc atgaacttat cgaggaagaa ctccgggttg
      781 cgatagatgt ccagaaacgc gtacgcggag gtcaagtcga gcggagtcaa cgcaaacatt
      841 ctcgcgatct catcgaagcc tcttacatcg ccgatgaatg ccttcgcggc cttgatgggc
      901 aagaacagcc agaacgcgcc attgaacgca aagagtacgg ttgcgacggc ggcgatgcgc
      961 ggcgccgttc cggtccggaa gatcgtggcg aggcgatacg cggcgacagc gagaagggta
     1021 agggcatgcc agttgacgaa cgccatgacc cggaagtaat ccacgcgaag cagatcgccc
     1081 agatagagga cgacggcgtg gtaggcccac atgtactgga gctttatccc ggcaaaatac
     1141 gggtcctgcg gcgggacacc gaagtcccgc atctggagca cgacggcggc gtggaaccag
     1201 gcgtcggagc gtatgcgcca ccactcgcgc gtgagcggta gtattgcgac cagcacaacg
     1261 gtggcgccca ggagcaccag gaagagcgaa agctcccggc gcggtggcgt tatgaccggc
     1321 ccggcccggc gcacacagga ggcgagggct gccagcgcgg tcacggcgac ccacgcggcg
     1381 atgctcaagg tggcggtgtg cgtgaccaat cccagcaacc ccacgccgac aagggtgacg
     1441 ggcgagagca caagcgctgc ctggagagcg tccacggcgt gcggccgccc gcgttccacc
     1501 aggaggtaca gcgccattcc cggtaccaac atgagatgga tccacgtcag cacctgcagc
     1561 ggcagcatca gtaccgccgg cacggcaacg ccgaatgcga gcacgagcgc cgatagggtt
     1621 gtcagggtaa cacccgcgac gacacccctc gtaaatcgag acgacgcaat caatgatgcg
     1681 agggtcatgg ttacggggtc gacgggagag ttgctgatga gaagatcgtc ctggcgtact
     1741 gcgagtactg ggtactacgc gtaacatgct ttattgtaag ggatacacgc ttgaaagctc
     1801 aagtaacgtc tcaaccaact acgaccccat caccgtgaca tcgatcggac gcaatgcggg
     1861 gcccctcgca tcgagcagaa ccacggcctc ccacgttccc agcccgatcg ccccgtcccg
     1921 cacgggaagg gagatgcact cggccaggac gccagcgggc gagtagcccg caccctcggc
     1981 gtcgacccga tcgggccgtt cgccgggctc gtcgccaccg tcgggagcat cgcccgggcg
     2041 ccccgcgagg tggctccacg ccagccgcag caggtcgtcg acgtcttcgt cgagctcccc
     2101 gctcagggtc aggcacgctt cgctggatgg ggcgctgaga acgcacaggc cattggcgat
     2161 tccgctcgcg cgcacgaacg aattgacgtc cgaggtgacg tccgagagcg aagcgccttc
     2221 gtcggtcgcg atttccaggc gtgtggtggc gacgatcatg gagtggtgcc gggtccgtgg
     2281 ggggggaaag cgtgttgcat gtttgccggt ggcgtcggat actagggccc ggagtcccgc
     2341 ggcccgaacc atcgaaggga cgcgcctttg ggcgcgttcg cgatctgtct ccgcgacccg
     2401 tcactcgtgc gggacccggg cgcgcgctgc gtgcctgact ttaccagagg gatcccatga
     2461 cgaaaaagag ttttcgcctt ccgttcggcc gccccgagga tgccacgggc gacgagctct
     2521 cggtgctgcg tgaggaaaac cgccagctca agcgcgcggt cgaagagctc tcgatcctta
     2581 acgagctctc gcgcgccatc ggggcctcgg tcgactccga ggacatcatt cgcaagatcg
     2641 tcgatcgctc catgcgcgcc gtccaggccg agcagaccgt ggtcaccctg gtcgaccgca
     2701 acgccaacca gccgatgaag accttggtgc gggccatgac cagctcggcc gagcacccgc
     2761 actaccacgt gaacgagaac ttgctgggct ggatgcacct gtacaagaag ccgctcttga
     2821 ccaacgatcc caccaccgac gagcgcttcc ggggtctcaa gtgggaccag accatcaagt
     2881 cgatcctgtg cgtcccgctc atggtcaagt ccgagttgat cggcgtcatc acgagctaca
     2941 acaagaaggg gcagggcgga ttcaacgaag aggaccagcg cctgctcgcg atcatcgccg
     3001 gccaatccgc gcaaatcatc gagaacgcac gcctgtacga agaggaaaaa gctctcgtca
     3061 gcatgaagga gcaagtcaag ctggcggcgc agatccagca ggatctgctg ccgaaggccc
     3121 cgccgcgcct cgaggggtat gacatcgccg cgtgcagcat ggcggcccag atggtgggcg
     3181 gcgattacta cgacttcgtc cgcgtgcgcg acgggcgctg ggcggtgtgc ctgggcgacg
     3241 tctcgggcaa ggggcttccc gcatcgcttt tgatggcgac gctgcaagcc acgttgcggg
     3301 gtcagaccgc gctggagcac gccgtgcgtg aaaccatctc gcgctccaac cgtctcattt
     3361 accagagcac ggatccggag aagttcgcga cattgttctt cgcggtcctc gacaccaacg
     3421 ggggagggtt agccttctgt aacgcgggac acgagtaccc gatgctgttc aagaagggcg
     3481 gcggtccacc ggcgcgcttg atcacaggag gcatggcact cggggtcctg gaagagttcc
     3541 cgtacgaaga agacgcggcg cgcatggacg cgggcgacac gctgatcgtg tattcggacg
     3601 gcatcaccga cgccgtcaac gagttcgacc agcccttcgg cgaagagcgt ctccagaagt
     3661 gcgtcaccga ccacgccggc gagcgcgcgg acgagatcat gaatcgtgtc gtggccgccg
     3721 tgaaggcgca cgagggggtc gcgccgcgca tcgacgactt gacgctcatc gtggtcaaac
     3781 gggtgtcgta gccgcgaata ctgcttgaaa ccgatgtccc ctcccggcgt atctagggga
     3841 ggcgccgcgg atcccagcgc ttgaattcga gcccatacgt atgatcggtc aacgcgtaca
     3901 gcactatcag attaccgaga agctcggcga gggcgggatg ggtgtggtct atcgcgccca
     3961 ggatacccgt ctgaaccggg ccgtcgcgct caagttcctc cacccggcca tcgtcgcgag
     4021 ccccgacgac cagcagcgcc tggtccagga ggcgcgcgcg gccgccgcgt tgagccatcc
     4081 caacatctgc acggtctatg agatcaacga acacgatggc cgcacgttca ttgccatgca
     4141 gttcgtggag gggtcgaccc tgcgcgatcg cgtgctttcg ggacgaatcg agaccgtgga
     4201 tgccctgcgc tacttgattc aaatcgccga tgggctgaag caggcccacg agcaaggtat
     4261 cgtccaccgc gacatgaaga gctcgaacat catgatcacg ccctcggggc gcgcggtgat
     4321 catggacttt gggctggcgc gtccggccgg taccggccgc accgacgagc gtttctcgtc
     4381 gcgcggaacg tcggcctaca tgtcgcccga gcaggcgcgc ggtgagatgg tcgaccagcg
     4441 caccgacatt tggtcgctgg gcgtggttct ctacgagatg ctggccgggc gcttaccgtt
     4501 tcgcggcgac tacgagcagg cggtggtcta ttcgatcctg aacgagcccc accgcccgat
     4561 cgcggagttt cgggcggggt tgccggccgc ggtgaccaag gtcgtggaga cgtgcctggc
     4621 gaagaatccc gccgatcgct accagagcct cgacgcgctg atccacgccg tacgaggggc
     4681 cctcgacgac atgagcgggc tgcgtcgccg ggacggcgag cgctggggcc gggcgcgcct
     4741 cgcgggcgtg gccgcggcgg ccgtgctcac ggtggcggcc ggcttcgtgg tcttcgactt
     4801 cctgcgcggc gggcgcgcgg gcggcgaaga gcgcgtaccc atcgccgtga tcgatttcaa
     4861 caacgagacc gacgaggccg cgctggacgg actctcgggc atgctgatca ccgcgctcga
     4921 gcagtcgcgc cgcctctcgg tgatgacgcg ctcgcgcatg ttcgacatcg ccaagaccat
     4981 cggacacggg gacgccgctc gcatcgacga agcgctcggg cagcgcatct gcgaagccgc
     5041 cggcgtggac gcgctcgtga tcccgaccat tcgccgcttc ggcgaccttt actccatcga
     5101 cttgaaggtg atggacacgc gccggcacaa ctatgtgttc accaccaagg aagaagggcg
     5161 cggcctcgag agtatcccca acatggtcga cgccatcgcg cgcgacatcc gcatcgacct
     5221 gcgcgaaacc acggagtcgg tggcgcgtac ggccccgttg gccgacgtca ccaccaccaa
     5281 cctgggcgcg tatcaggcct atttcgaggg cgaagaattg ctcgaccgct tgcagttcac
     5341 tcccgcggga aaggcgttcg agcgcgcgat tgcccgcgat tcgaccttcg cgctcgcata
     5401 ctaccgcctc gcctacaccg agtggtggtc gcgccagaac gcgaaggagg ccaaacagca
     5461 catcgactac gcgatcgcca acctggatcg cattccgcag aaggagcgct acctcgtgcg
     5521 cgcgctcaaa gtggcgctcg acgacggctt cgaggcccag ctgccggtgc tcaaggaaat
     5581 gcgcacgtta tatccggacg acaaggagat gctgttcggc ctcggcgacg cggagttcca
     5641 ctccaacgag ttcgattcgg cggaggtcca tttccgcgcg gcacttgcga tcgatcccga
     5701 aatggagcgc gcgttgcagc acttggcctg gacccttctg cgcttggagc gttacgaaga
     5761 aacgctggtt atcgcggagc gctggacgaa agcggccggc tcgagcgaag cctacgagtt
     5821 cctcgccatc gcccatatgc gcctgggccg tcccgacacg gcgcttcgaa ttctcgacga
     5881 gacgattgcg cgcgaccccg agaatccgaa gctggtcgtg cgcaagtcgc tggtgtattt
     5941 tacgacccac cggccgaacg aatcgctggc ggcactggac caagccgtcc gcctgggatc
     6001 gaaggacccg gtcgtccaac tcgagattgg acagatgcgc tcgatggtat tgtacccgta
     6061 tctgggacgc tatcgcgagt cggctcgttt gctcgacgcc gcgcgcgcgg tgtcgttgac
     6121 gaccttcggc gactccaccg ccgcggtcaa cctcgccgtg gccgaacgtg ccctcgcgta
     6181 ctgggcgcac caggacgccc ggcgcgccat cgcggatctg gaagcgctgc aaacggtgcg
     6241 cgcgaaactc ctcaccgaca actattggct gacgctgtcc gcgttccaca tgatcgccgg
     6301 ggatacggcg gaagcggggg cgatccagcg cgcgcactcc aagaatctca ctgaggaaca
     6361 gcgtgcgtcg atcggcgtgt tcaaggcggc gacgtccgga gattgcgagg gcgccgaggc
     6421 gctcatgcgc tcggcggccg ccggcaaggg agtacccggt tcgtcggagg acgatcgcct
     6481 ctatgtgatc gggcgctgcc agctggcggg aggcgcgtac gacggcgcca tcgcgtcgtt
     6541 gcgccgcatc ctcgatcgcc cggtcacttt cgcggattcg gggccgatga tcccggtggc
     6601 gtggttctat ctcggcgaag cctacgagcg aaaaggcgac gtcgccaagg cggcggcggc
     6661 gtacgaccgc gtcctcgaga tttggaaaga cggcgacgac gatctgtact gccgccgtga
     6721 agcccggatt cgactggacc ggctcgcggg cacgcgctcg atgtaatggt agcgttccgc
     6781 tcgtcgcacg tcgtgctaac gatcacgttc gcggtaacgt tggcgtgcaa tcgatctcca
     6841 tcgcaacccg ccgatctcat tctcaataac ggcatcgtgc acaccttcgc gggcagcgcg
     6901 agttcgaacg cgcgcgccac ggcgattgcg gtaaggggcg agcgcatcgt ttatgtgggc
     6961 gacgatgcgg gtgcagccgc gtggcgcggt aatgagacac gcaccgtcga tctcgcgggg
     7021 aagacggtgc tgccgggact ggtcgacgcg cacggtcacc tggccaacct cgggcgttcg
     7081 ctggaggagg tgcagttggt cggctcgacc tcgcgcgagg atgtcttgcg gcgcgttcgt
     7141 caaacccagc tgcagatccc ggccggcgaa tggatacacg gacgcgggtg ggatcagaac
     7201 gattgggagc aaacgtcgtt tccgacctgg agggatctcg ccggaaccga ggccaacccg
     7261 gtttacttgg agcgcgtcga cggccacgcg gtgtggctca atcggacggc actcgacgcg
     7321 tgcggcatca cgcgcgcgac accggatccg cgcggtggca ggatcgagcg cgacgcaaac
     7381 ggggaaccga ccggcgtcct ggtcgacgag gccgagaagt tgctggtcgg aaacgtgccc
     7441 gagccaaccg atgacgactt ggatcgccgc ctcgcgcgcg cgatcgcgga gtgcaaccgc
     7501 ctgggattga cgggcgccca cgacgcgggc accgacccca acgtgctcgc gtcgttgcgg
     7561 cgtttgggag aacgcggcgc gctcaccatc aacgtgtact gcatgctcga ctccgacgcg
     7621 ccggcgctcg tgcgttcatt cttcgcgagc ggcccgagta tcgagtacga gggacgcctg
     7681 gtggtgcggt cggtgaagct gcgcgccgac ggcgcgctgg ggtcgcgggg tgccgcactc
     7741 ttgcgcccct
//
LOCUS       DASPGK010000068        14586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_837163, whole genome shotgun sequence.
ACCESSION   DASPGK010000068 DASPGK010000000
VERSION     DASPGK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14586
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837163"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1208
                     /gene="rlmN"
                     /locus_tag="VEC56_01770"
     CDS             141..1208
                     /gene="rlmN"
                     /locus_tag="VEC56_01770"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXV12912.1"
                     /translation="MERTHLRNLPLAHLRELVLSEGEPEFRFRQLATWVYARLESRFE
                     GMSDLPHDFRERLATRFDIAASAPVSIQQSAVDGTRKFLFEFADGARVEAVLMRYDRR
                     NTVCLSSQVGCPLDCVFCETAKGRFQRNLTAGEILDQLCLLKGEAGPPSHKINVVFMG
                     MGEPLLNLPNVIDAIRVMNDPLGLDLGGKRITVSTSSFPDRIRELADSGVDCSLALSL
                     NAPNDEKRRELMPKASHFPIAELLESAREFVRDGRRRVTLEYVLIKGRNMSDEDADQL
                     GALAQGQPFKLNLIPYNPGRHDPFQRPTEPDIDRFVRRLLPVAPTVTVRRSRGVDIDA
                     ACGQLWNQYLMQKKKPAKGAA"
     gene            complement(1273..2322)
                     /locus_tag="VEC56_01775"
     CDS             complement(1273..2322)
                     /locus_tag="VEC56_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12913.1"
                     /translation="MEIDIPQSGLRRALAGAFWLCLAGSPIPAAAGVEQVALANGLSA
                     TIYPADYLRDRVVTTGDESAIELDDERMIPVIVDINDPSIVNKGDGEFHPFSVDDVLA
                     TLRALDHRSVRMSIRVYLLPYPRRSLLVSSTSGGELFLSPHVLSIDRSVAAYIVAHEL
                     GHAFHNRFMADGSPAWDEYRLLRGITDTARFHDRASHAYRPKEILAEDFRVFFGGEAA
                     YFGGYVENPEIARPEAVMGLENFFLRVAAEPVAPRAPRIFATSFPNPFNPATEVRVSL
                     PDELTGKDQRVSVRVYSVTGALVRDLYDGRATGDFAVRWDGTDNRGNRVASSTYYAAV
                     IAGGSKETVKLVLLK"
     gene            complement(2618..3949)
                     /locus_tag="VEC56_01780"
     CDS             complement(2618..3949)
                     /locus_tag="VEC56_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV12914.1"
                     /translation="MRCSFARGTVACLALWAAVGSGPVEPRAAAQARFDVDAYRWAQG
                     RIIDTVYVHGNTRVKTFAVLREMESQPGQRLDAVSVDRDQRYLGDLSPFATVAIHVEP
                     IGEDRCVLSVVVKERPTLLLKFIYPVLDYDINTERILYGLKWFDRNFRRRLENFSLEF
                     LRDNRENDAASAQWSTAWIGWRHVGVSARASYFNRGEPASETTIIKQTRGAANVSIPL
                     TERRIAFSQIIAGVAIADNHVGLSDADFAREELLSPSLGFRYDGRDGTVKPRRGGYFY
                     VNLTGNRVLNGEGSTYYRVDNDIRFFHALDATTVVGLRSAASIQIGEFPEYVRFGIGG
                     PGTIRGYERSDFRSTQRWVQSLELRMMPWPKRLYKLPILGTTDFQFGLVAFIDTGIGW
                     TRESEFDLNNFHSGFGFGVRMFSPIQDVIRVDVGFTPQGDIRPYFTTGSNF"
     gene            complement(4015..5157)
                     /locus_tag="VEC56_01785"
     CDS             complement(4015..5157)
                     /locus_tag="VEC56_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12915.1"
                     /translation="MRCSLFNSIRFACAVLIGASALAGVPSSRAKAGSPDSASIDAPA
                     PPPPPTRKRIVIGGSGVRIEDEGISIEADTTVDFNFDFKYDEEEYGDDIVKVGESVFV
                     AAGDVVAGDVVVFGGNAIIEGTVAGSVVVLGGEIRARRGSEIKGDIVAIGGTIEEDED
                     VIIRGERILIGGVAGRIGDQLDISTRGLRVLIVAVSLFVTLVLYFITMLFLRARVLRA
                     SEHLSTSLLKCFGAGVLASVLGSFALLIVMIPLIITIVGIPLAVILGMSWLGVVTIAG
                     TVWVYALGRTIAARMGTDAGVFARLAIGLAVAIIPAIMSFMIEAVGGPTGLSVFFQVV
                     SFMVWFFGFLIGLGAIVLSRFGTRPPVNTEPPLARSMPELAPNPGV"
     gene            complement(5138..5764)
                     /locus_tag="VEC56_01790"
     CDS             complement(5138..5764)
                     /locus_tag="VEC56_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV12916.1"
                     /translation="MNCETAKNLIQPYLEGRLPTLERNEFVYHVTECGACETEVLAYR
                     QVFRSLRSMERFEAPSRVQVAVLAHLHAEGLIHEPRFPMFKRVLDAFLDLPARVRYPL
                     AALAAVMVMYLPVGFVLAGARGSLAGAAETLARGVLWFQGVFEGVSARANVEPYTRAA
                     RTVTHAAAEVALPIAVMLLVAALVAVVALTAARIVRRKKPSGHALFTF"
     gene            complement(5791..6870)
                     /locus_tag="VEC56_01795"
     CDS             complement(5791..6870)
                     /locus_tag="VEC56_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV12917.1"
                     /translation="MAGTVWLSLAGVAVAGPGHRLEREEKSSVSATGKKSLVVKNARG
                     RTVVVGRTDATTVSIVALKTAVGNDKDAAAEMLDKITVQVTERGDEVVIETRDDGKWD
                     NDWSLWSVVKGGRRSAWVDYTIEVPHAFKVAASTTSGEVRVSNLQGAAEVTATSGDVS
                     MRAVGGGSSARMTSGDLEVIDVAGDLSVSATSGSVVVDNIKGKLEIEGTSGDFRAARV
                     GKDADIQLASGDFMLEGCSGNVSFRAASGDAVMREIDGSVDASSSSGDIEVLIIPVAE
                     RSFSLSTSSGDVIVYFVPVKGFGFQLDVRTSSGSIEGDLPIKVTRADRRRLQGVVGSG
                     AARVDIETASGDVTITERAEAADNR"
     gene            complement(7124..7699)
                     /locus_tag="VEC56_01800"
     CDS             complement(7124..7699)
                     /locus_tag="VEC56_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV12918.1"
                     /translation="MKEEDILLVAKARSGDERAYRTLLQKYERAVFSICLRMVRNRDE
                     ASDLAQESFIKVFHSLDRYNPQYAFSSWLFKITSNLCIDHLRKRRIDTFGMDDPIDGE
                     KGEIQRQYVAPGPNPEETYVRGEKVKRLEEAIAALPEHYRIMLVLRHQEDLSYEEIAD
                     TLSIPLGTVKARIHRAREMLKGLLDDAEFLA"
     gene            7938..9221
                     /gene="eno"
                     /locus_tag="VEC56_01805"
     CDS             7938..9221
                     /gene="eno"
                     /locus_tag="VEC56_01805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXV12919.1"
                     /translation="MTIIEYVHAREIMDSRGNPTLEVDVGLDGGALGRAAVPSGASTG
                     KHEALELRDGEHTRYMGKGVHKAVSNVNDVIGPEIIDLDAIDQAYIDQLMLELDATAN
                     KARLGANAILGVSLAVARAAAESMNLPLYQHIGGVFARTLPVPLMNVVNGGAHADNNV
                     DIQEFMIVPVGAPNFAEALRMGAETFHALKKVLRDKKYATSVGDEGGFAPNLRSNEEA
                     IEVLLEGIARANYTPGKDVCIAIDAASTEFYQNGTYLLRGENRKLSSEQMVQFWADWV
                     KKYPIVSIEDGMAEDDWDGWAQLTSALGGRVQLVGDDLFVTNEERLRRGIDAGVANAI
                     LIKLNQIGTLTETLHSIEVAKSAGYACVISHRSGETEDTTISHLAVATNAGQIKTGSL
                     CRSERIAKYNELLRIDEELGSVAMFPGAAAFPAFA"
     gene            9259..9711
                     /locus_tag="VEC56_01810"
     CDS             9259..9711
                     /locus_tag="VEC56_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXV12920.1"
                     /translation="MQYWKTEQDATGDAGKFHDYRPRARAPHAHPSVVASGGSVGPFL
                     RKFYRHQVVVSAPVQRFFLFLLLAGVIYAFVLGDGGAIRIAMLRHDRSSLDRSIAELQ
                     RNAALLESEIALLDSDEFYIEKTGRERYGYIRPGEKVYKIVPTDSKSK"
     gene            9831..9907
                     /locus_tag="VEC56_01815"
     tRNA            9831..9907
                     /locus_tag="VEC56_01815"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9865..9867,aa:Met,seq:cat)
     gene            complement(9962..10312)
                     /locus_tag="VEC56_01820"
     CDS             complement(9962..10312)
                     /locus_tag="VEC56_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV12921.1"
                     /translation="MMFERSMMNDVCVLTPKRNLTGGEETRALTDAVEEITAGGVTKV
                     VIDFGKISWVNSMGLAGLQRARLACVSHGAAFALASVGGRIKNLMLTTRLVILFDTYE
                     TLDEALAGQESTNA"
     gene            10512..11447
                     /locus_tag="VEC56_01825"
     CDS             10512..11447
                     /locus_tag="VEC56_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HXV12922.1"
                     /translation="MMDTTTIDSKSNVGAGIWRLADPKISLASIASMTLAAGAAVRAG
                     EVSMVWLVLTVLGILAIEVAKNASGEVVDWDSGTDAAIRSDERTPFSGGKRVIVDGLL
                     TRAQTVRVAAVSYAIGIAIGAVIAMVREPRVWYFGVVGVACAFFYHAAPLRLSYRGWG
                     EAAVAFSYGPLIACGTFLVQHHRIGGPVVPLSILLGVLIAAFLWVNEFPDARADAQAG
                     KRTLVVRLGKARAARIFAILAVGPFAVLPALVATGLPGGVWLALVGLLPALNAARIVH
                     SEPEHHARIAAAQASTLVAFLLFALGGAIGLAAWR"
     gene            complement(11460..13121)
                     /locus_tag="VEC56_01830"
     CDS             complement(11460..13121)
                     /locus_tag="VEC56_01830"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HXV12923.1"
                     /translation="MSKRPTGARTRPSDFPRGVDLLSDPLLNKGTAFTPEERDALGLR
                     GLLPPRPADMNEQIARVMTNYRAKPNDLERYIYLTSLHDRNETLFYRVVVDHIEELLP
                     IIYTPTVGRACQVYGQIFRRARGLFVTADDRDRVKSVLSNWPHADVRAIVVTDGERIL
                     GLGDLGANGMGIPVGKLALYTACAGVHPAVTLPVTLDVGTDNESLLDAPLYLGLRRHR
                     MRGAPYDALVEEFVTAANELWPGVLIQFEDFGNQNAFRLLAKYRERVCSFNDDIQGTA
                     AVTLAGVFSALRVTGGDLRRQRFLFLGAGEAGVGIADLIVSAMVDAGMSREDARRACW
                     LVDSHGLVVRSRKDLAEHKRPYAHEAPGAANLLDAIRALEPTALIGVSGQPSTFTREI
                     LEAMAKLNERPIVFALSNPTANSECTAEEAYTHTGGRALFASGSPFATVTFQGKQMIP
                     SQANNAYVFPGVALGIVASRIARVTDEMFARAARTLAGLVSKDDLANGLLFPPLPSLR
                     GVSLEIAAAVARVAFERGLAGTAEPRDLKGFIREQVFDPEYQSYV"
     gene            complement(13251..14396)
                     /locus_tag="VEC56_01835"
     CDS             complement(13251..14396)
                     /locus_tag="VEC56_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV12924.1"
                     /translation="MQRIGHASGSVCVRSFGLARAATVVVLSWAALGHPRPACAVDAT
                     VLMYGVKPATSFDLIRNGVPVSAGASTALGSLAFVMDVSTGTLVAIAPVGDLAPPAPP
                     LFTSVEVGLPGCATASWMQSGDPTVIGYVVAYGSQSVAGGGASNYENMIEVGATGSAE
                     VCFLPLGRQYFAVRAKNIGGMLSAYSVERSVDIVIVSVLISRFDARPRGDGVHLEWQV
                     EADEVVSGYRVYRGAAGEAQRVLNDALLPSDATSFVDASVRGGVAYTYVLAAVKENGE
                     EVFSIPVVAMVAPLSFDLEPNVPNPFNPTTRIGFTLPESARAILRIYDVRGSHVATVL
                     DSPLGAGRHHVEWNGVDDAGTPVASGTYFYTLAAGKRTVARKMVLVK"
BASE COUNT         2577 a         4804 c         4756 g         2449 t
ORIGIN      
        1 gtcagcaatc aggatcgtct tgggcatacg agggttggca atcgaagcgg cgacaacaac
       61 attcggtgtt ttttagggat accacattgc ctataattta accacactga aatcgggcgg
      121 cgttctctcc gccccacgcc atggaacgaa cgcatctacg caacctgccg ctcgcgcacc
      181 tgcgcgagct cgtcctgtcc gagggcgagc cggagtttcg ctttcggcag ctcgcgacct
      241 gggtgtacgc gcgcctcgag tcgcgcttcg aggggatgtc cgatctgccc cacgattttc
      301 gcgagcggct cgcgacgcgc ttcgacatcg cagcgtcggc gccggtgtcg attcagcaat
      361 ccgcggtcga cggcacgcgc aagttcctgt tcgaattcgc cgacggtgcc cgcgtggagg
      421 cggtgctcat gcgctacgac cgtcgcaaca ccgtctgcct ctcgtcccaa gtcggctgtc
      481 cgctggattg cgtgttttgc gagacggcca aggggcgctt ccagcgcaac ctcaccgcgg
      541 gcgagatcct cgatcagttg tgcttgctca agggcgaagc cggcccgccg tcgcacaaga
      601 tcaacgtcgt gttcatgggc atgggcgagc cgctcctgaa tctccccaac gtgatcgacg
      661 ccattcgcgt gatgaacgat ccgctcggcc tggatctggg gggaaagcgc atcacggttt
      721 cgacgtcgag ttttccggac cgcatccgcg agctggcgga ctccggggtg gattgctcgc
      781 tcgcgttgtc gctcaacgcc cccaacgacg agaagcgtcg cgaattgatg ccgaaggcgt
      841 cccacttccc catcgccgag ctcctggagt cggcgcgcga gttcgtccgt gacggacgcc
      901 ggcgcgtgac cctggagtac gtgctgatca aagggcgcaa catgagcgat gaggacgcgg
      961 accagctggg tgcgctcgcg cagggccagc ccttcaagct caacctcatt ccctacaacc
     1021 cgggacgcca cgatccgttt caacgcccga ccgagccgga catcgatcgc ttcgtccggc
     1081 gcctccttcc ggtcgcgccc acggtcaccg tgcgccgaag ccgcggcgtc gacatcgacg
     1141 cggcctgcgg ccagctctgg aaccagtacc tcatgcaaaa gaagaagcct gcgaaagggg
     1201 cagcgtagtc gatcggggag gagaatcgcg actagcccca tttcgcaggc ttgaatgacg
     1261 tgcgacggcc gctcacttga gcagcacgag tttcaccgtc tccttcgaac cgcccgcgat
     1321 gaccgcggca tagtaggtcg aactcgcgac gcggttgccg cggttgtcgg ttccgtccca
     1381 gcgcaccgcg aagtcccccg tcgcgcgtcc gtcgtagagg tcgcgcacca aggcacccgt
     1441 caccgagtac acccgaaccg aaacgcgctg gtctttgccc gtcaactcgt cggggagcga
     1501 gacgcgcacc tcggtggccg gattgaacgg attggggaag ctggtcgcga agatgcgcgg
     1561 cgcgcgcgga gcgaccggct cggcggccac gcgcaggaag aaattctcca ggcccatcac
     1621 agcctccggg cgcgcgatct ccgggttctc gacatacccg ccgaagtacg ccgcctcgcc
     1681 gccgaagaag acgcggaagt cctccgcgag aatctccttg ggacggtagg cgtggctcgc
     1741 ccgatcgtgg aagcgcgcgg tgtcggtaat gccgcgtaag aggcggtact cgtcccacgc
     1801 cggcgaaccg tcggccatga atcgattgtg gaatgcgtgg cccaactcgt gcgccacgat
     1861 gtacgccgcg accgagcgat cgatcgagag cacgtgcgga ctcaagaaca actcgccgcc
     1921 gctggtcgaa ctgaccagga gggaacggcg cgggtacggc aagagataca cgcggatcga
     1981 catgcgcacc gagcggtggt cgagtgcgcg cagtgtggcc agtacgtcgt cgaccgaaaa
     2041 cgggtgaaac tcgccgtcgc ccttgttcac gatcgagggg tcgttgatgt cgacgatgac
     2101 ggggatcata cgctcgtcgt cgagctcgat ggcgctctcg tcgccggttg tgaccacgcg
     2161 atcgcgcaga tagtcggccg ggtagatggt ggcggagagt ccgttggcga gagcgacttg
     2221 ctcgacgccg gcggcggcgg ggatcggcga gccggcgagg cacagccaaa atgcgccggc
     2281 gagcgccctt cgaaggccag attgcggtat atcgatctcc atacgctatg tcactcgatc
     2341 tcctccaact ccttgtagtg catcgagtat tccccgcggg ccgagccgcc catggacacg
     2401 cggcgtcagg caccgtgcgc gagcgggtcg tggccaataa cgtgtttatt taattatggt
     2461 taccgaagat acgtccgccc atgcgacgcg gggacgaggg ccgaccgcgg gtcggaaagg
     2521 ggcgcctttg ggcgaccgat acggggcttt cgacaccgga cctgggggtg gtcgggcaat
     2581 cccacccaag ggtccgtcga ggagcggagg cctcggccta gaaattggag ccggtggtga
     2641 agtaggggcg gatgtcgcct tggggggtga acccgacgtc gacgcggatc acgtcctgga
     2701 tcggcgagaa catgcggacg ccgaatccga atccgctgtg gaaattgttg aggtcgaact
     2761 ccgactcgcg cgtccaaccg atgccggtgt cgatgaacgc caccagtccg aactggaagt
     2821 ccgtggtgcc caggatgggt agcttgtaga gccgcttcgg ccacggcatc atccgcagct
     2881 ccagggattg tacccaccgt tgcgtggatc ggaagtcgct gcgctcgtag ccgcggatgg
     2941 tcccggggcc cccaatcccg aagcgaacgt actcgggaaa ctcgccgatc tgaatcgagg
     3001 ccgccgaccg caaccccacc acggtggtgg cgtcgagagc gtggaagaac cgaatatcat
     3061 tgtcgacccg atagtacgtg ctgccttcgc cgttcaagac ccggttgccc gtgaggttca
     3121 catagaagta tccaccccgg cgcggcttca cggtgccgtc gcgaccgtcg taacggaacc
     3181 ccagcgacgg gctcaggagc tcctcgcgcg cgaagtccgc gtcgctcaag cccacgtgat
     3241 tgtccgcgat cgccaccccg gcgatgatct gcgagaacgc gatacgccgc tcggtgagcg
     3301 gaatggacac gttggccgcc ccgcgcgtct gcttgatgat ggtcgtctcc gacgccggct
     3361 cgccgcggtt gaagtacgac gcgcgcgcgc tcacgcccac gtggcgccag ccgatccacg
     3421 ccgtcgacca ctgcgccgag gccgcgtcgt tctcgcggtt gtcccgcagg aactcgagcg
     3481 agaagttctc gagccgccgc cgaaaattgc ggtcgaacca tttcaacccg tagaggatgc
     3541 gctcggtgtt gatgtcgtaa tcgaggaccg ggtagatgaa cttcagcagc agcgtggggc
     3601 gctccttgac caccacactc aacacgcagc gatcctcgcc gatgggctcg acgtggatgg
     3661 caacggtggc gaagggagaa aggtcgccca gatagcgttg gtcgcggtcg accgagaccg
     3721 cgtccagacg ctggcccggt tgggactcca tctcgcgcag aaccgcgaac gtcttgacgc
     3781 gcgtgtttcc gtgcacgtac acggtgtcaa tgatccgccc ctgggcccag cggtaggcgt
     3841 cgacgtcgaa gcgggcctgg gcggccgcgc ggggctcgac gggcccggag ccgacggccg
     3901 cccacagcgc gagacacgcg accgtacccc tcgcgaaaga acaccgcata cgtatctcaa
     3961 ggatacgccg cggccaccgc gacgccaaag tgaaacctgg gagttcgaga ggtgctagac
     4021 gcccgggttg ggggccagct cgggcatgct acgcgccagc ggaggctcgg tattgaccgg
     4081 cgggcgcgtg ccgaaacgac tgagcacaat ggcacccaag ccgatcaaga acccgaagaa
     4141 ccagaccatg aatgagacta cttggaagaa gacggagagg ccggtcgggc caccgacggc
     4201 ttcgatcatg aacgacatga tcgcggggat gatggccact gcaagcccga tcgcaagacg
     4261 ggcgaacaca cccgcgtcgg tgcccatgcg cgccgcaatg gtgcgaccga gcgcgtagac
     4321 ccacaccgtg cccgcgatgg tgaccacacc gagccaactc atgccgagga tcaccgcaag
     4381 cgggattccc acgatggtga tgatcaaggg gatcatgacg atcaagagcg cgaacgatcc
     4441 caacaccgac gccagcaccc ccgcaccgaa gcacttgagc aggctcgtgg agagatgctc
     4501 gcttgctcgc agtacgcggg cccgcaggaa cagcatcgtg atgaaataga gaaccagcgt
     4561 gacgaacagg ctgaccgcga cgatcaacac gcgcaaaccg cgcgtgctga tatccagctg
     4621 atcgccgatg cgaccggcca cgccgccgat caggatccgc tcaccccgaa tgatgacgtc
     4681 ctcgtcctct tcgatggtgc cgccgatcgc gacgatgtcg cccttgatct ccgagccgcg
     4741 gcgcgcgcgg atctcgcccc ccaagacgac caccgagccc gcgacggtac cctcgatgat
     4801 ggcgttgcct ccaaacacga ccacgtcgcc cgccaccacg tcgccggccg ccacgaagac
     4861 gctctcgccc accttgacga tgtcgtcgcc gtattcctct tcgtcgtact tgaagtcgaa
     4921 attgaagtcg acggtggtat cggcctcgat cgagatgcct tcgtcttcga tgcggacgcc
     4981 gctacccccg atgacgatgc gcttgcgggt gggcgggggc ggcggtgcgg gggcgtcgat
     5041 cgacgcggag tcgggcgaac cggccttcgc cctcgacgaa ggcaccccgg ccagcgcact
     5101 cgcgccgatc agcaccgcgc aggcgaaacg aatgctatta aaaagtgaac aacgcatgac
     5161 cggatggctt cttgcggcgc acgatgcgcg ccgcggttaa cgctacgacg gcgaccaggg
     5221 ccgcgacaag cagcatcacc gcgatcggca acgcgacctc ggcggccgcg tgcgtgacgg
     5281 tgcgtgccgc gcgcgtatac ggctcgacgt tggctcgcgc cgacacgccc tcgaaaacgc
     5341 cctggaacca cagcacaccg cgtgccaggg tctcggccgc gcccgcgaga gagccgcgcg
     5401 cgccggcgag gacgaatccc accggcaggt acatgaccat gaccgccgcc agcgccgcca
     5461 gcggataacg aacgcgcgcc ggcaggtcga ggaacgcgtc gagcacgcgc ttgaacatcg
     5521 ggaagcgcgg ttcgtggatc aagccctcgg cgtggagatg cgcgagcacg gctacctgca
     5581 cccggctcgg ggcctcgaaa cgctccatgg aacgcagcga acggaacacc tggcggtatg
     5641 cgagcacttc ggtctcgcac gcaccgcatt cggtgacgtg gtagacgaac tcgttgcgct
     5701 cgagcgtcgg caaccgacct tcgaggtacg gctggatcaa attcttggct gtttcgcagt
     5761 tcatggctct ttcacacgac agattgccta ctatcgattg tcggcggctt cggccctctc
     5821 ggtgatggtg acgtcgccgc tggcggtctc gatgtccacg cgcgcggcac ccgaaccgac
     5881 cacgccctgc agacggcgcc ggtcggcgcg cgtcaccttg atgggcaggt caccctcgat
     5941 cgagccgctg ctcgtgcgga cgtcgagctg gaacccgaac cccttcacgg gcacgaagta
     6001 cacgataaca tccccgctcg acgtgctcaa actgaacgag cgctcggcca ccgggatgat
     6061 caacacctcg atatcgccgc tcgaggagga ggcgtcgacg cttccgtcga tctcgcgcat
     6121 gaccgcgtcg ccgctggcgg cgcggaacga cacgttgccc gagcaccctt ccagcatgaa
     6181 gtcgccgctg gcgagctgga tgtcggcgtc tttgcccacg cgcgccgcgc ggaagtcgcc
     6241 gctggtaccc tcgatctcga gcttaccttt gatattgtcg accacgacgc tgccactggt
     6301 ggcagacacc gacaagtcgc cggcgacgtc gatcacttcg agatcgccgc tggtcatgcg
     6361 cgcggacgac cctccgccca cggcgcgcat gctgacgtct ccgctggtcg ctgtcacttc
     6421 ggccgcaccc tgcaggttgg acacgcgtac ttcgccgctc gtggtcgaag ccgccacctt
     6481 gaaggcgtgc ggcacttcga tggtgtagtc gacccacgcc gagcgacgac cgcccttgac
     6541 caccgaccat aggctccaat cgttgtccca cttgccgtcg tcacgcgtct cgatgacgac
     6601 ttcgtcgccg cgctcggtta cctgcaccgt gatcttgtcg agcatctcgg cggcggcgtc
     6661 cttgtcgttg ccgaccgccg tcttgagcgc gacgatcgag acggtggtcg cgtcggtgcg
     6721 accgacgacg acggtccgcc cgcgcgcatt cttgacgaca agggacttct taccggtagc
     6781 cgaaacggag ctcttctcct cgcgctcgag cctgtgaccg ggacccgcca cggccacgcc
     6841 cgcgagcgac agccacacgg tgcctgccac gagggctcgt ttaatcgagg ccccgttcgt
     6901 tgctctggcc aagtgagtga tcatcggtgt tcctcctgga ttcccgtgct gcgcggcgtc
     6961 gccatgacta ctgtggccgc ctcgcacatc tgctctacgg agccgggcgc caaaagttcg
     7021 cccggcacta aagttttcgt gggtcctgcg ccgactgggc cgagacctga cttccggcgt
     7081 aacccattat cggaactatc gatatgccca cgaatggcgc ccctcatgcg agaaactccg
     7141 cgtcgtccag cagacccttg agcatctcgc gggcccggtg gatgcgggcc ttgaccgtcc
     7201 ccagcgggat cgagagggtg tccgcgatct cttcgtacga aagatcctcc tggtgacgga
     7261 ggaccagcat gatccggtag tgctccggca acgccgcaat cgcctcttcc agccgtttca
     7321 ccttctcgcc acgaacgtac gtttcctccg ggttggggcc cggggcgacg tactggcgct
     7381 ggatctcgcc cttttccccg tcgatcgggt cgtccatccc gaaggtgtcg atgcggcgct
     7441 tgcgcaagtg gtcgatgcac aggttcgacg tgatcttgaa cagccagctc gaaaaggcgt
     7501 actgcgggtt gtaacggtcg agcgagtgga aaactttaat gaaggactcc tgcgcgaggt
     7561 cgctcgcctc gtcgcggttg cggaccatac gaaggcagat actgaaaacg gcccgctcgt
     7621 acttctgcag aagtgtccgg taggctcgct cgtcacccga gcgggccttc gccacgagca
     7681 ggatgtcctc ttctttcacg gtattggaaa tagacgagcg gccggccgtc tttccaggaa
     7741 ggcgcggcgg ttcacgcgag gcgtgatcgg tgcccggcgc gccgcggcaa ggcgcaccga
     7801 ctacggccac cggacatgcg gaaccgttcg gatggcgctg gagatcaccg ctccaagcgc
     7861 tgaaagtgat ttatgttgac acatttccga gtgcgtttct agagtttgtt tggcgtcctt
     7921 ccggcgcccc catccacatg acgatcatcg agtacgtcca cgcccgcgag atcatggatt
     7981 cgcgggggaa tcccacgctg gaggtcgacg tcggcctcga cggcggagcg ttgggccgcg
     8041 cggcggtccc ctccggcgcc tcgaccggca agcacgaagc tctcgagctg cgcgacggcg
     8101 agcatacccg ctatatgggc aagggggtcc acaaggccgt ctcgaacgtc aacgacgtca
     8161 tcggtcccga gatcatcgac ctggacgcca tcgaccaggc ctacatcgac caactgatgc
     8221 tcgaactgga cgcgaccgcc aacaaggcgc gcctgggggc caacgccatc ctgggagtgt
     8281 cgctcgcggt cgcgcgcgcg gcggccgagt cgatgaacct cccgctctac cagcacatcg
     8341 gcggcgtatt cgcgcgcacc ctgccggtac cgttgatgaa cgtggtcaac ggcggcgcgc
     8401 acgccgacaa caacgtcgac attcaggagt tcatgatcgt tccggtgggt gcccccaact
     8461 tcgccgaggc gctgcgcatg ggcgcggaga cgttccatgc cttgaagaag gttctccgcg
     8521 acaagaaata cgcgaccagt gtgggcgacg aggggggctt cgcgcccaat ctgcgctcga
     8581 atgaagaagc gatcgaggtg ttgctcgaag ggatagcgcg cgccaactac accccgggca
     8641 aggacgtgtg catcgcgatc gacgcggcgt cgacggagtt ctaccagaac gggacctacc
     8701 tgctcagggg cgagaaccgc aaactctcgt cggagcagat ggtgcagttc tgggccgatt
     8761 gggtgaagaa gtacccgatc gtgtcgatcg aagacggcat ggccgaagac gactgggacg
     8821 ggtgggcgca attgacctcg gcgctgggtg ggcgcgtgca gctggtgggc gacgatctct
     8881 tcgtcaccaa cgaggagcgc ctgcgccgcg gcatcgacgc cggcgtggcc aacgcgatcc
     8941 tcatcaagct caatcagatc gggacgctga cggagaccct ccactcgatc gaggtggcga
     9001 agtcggcggg ctacgcgtgt gtcatctcgc accgaagcgg cgagaccgag gacaccacca
     9061 tctcgcacct ggcagtcgcg accaacgcgg ggcaaatcaa gaccgggagc ctgtgccgga
     9121 gcgagcgtat cgcgaagtac aacgagctcc tgcgtatcga tgaagagctg ggctcggtgg
     9181 cgatgtttcc gggagcggcg gcgttccccg cgtttgcgta gcgcctccct gcggacccgc
     9241 gacgaaggag atcggacgat gcagtactgg aagacggagc aggacgcgac cggcgatgca
     9301 ggtaagttcc acgactaccg cccgcgtgcg cgcgcgccgc acgcgcatcc gtcggtggtc
     9361 gccagcggcg ggagcgtggg tccgttcctt cggaagttct accgccacca ggtggtcgtg
     9421 agtgccccgg tgcagcgctt ttttctgttc ctgctcctcg ccggtgtgat ctacgcgttt
     9481 gtcctcgggg acggcggcgc gattcggatc gcgatgctgc gtcacgaccg ttcgagcctc
     9541 gatcgtagca ttgcggaact ccagcgcaac gcggcactct tggagagcga gatcgcgctc
     9601 ctcgacagcg acgagttcta catcgaaaag acgggccgcg agcgctatgg ctacatccgc
     9661 ccgggcgaga aggtctacaa gatcgtgcct accgattcga agtcgaagta agccggttac
     9721 tcgcggccca ccccgcggac acgaagttgg cgtgcgcggg cggtcaatcg tgggcgttct
     9781 ttaatcgctt gacggcggag gcgttactag ttactctgcg ggaacattga cgcggggtgg
     9841 agcagtctgg tagctcgttg ggctcataac ccaaaggtcg caggttcgaa tcctgccccc
     9901 gctaccaact aggagaatcc cggtgtcgac gaaagtcggc accgggattt ctcgtttggg
     9961 gttacgcgtt ggtcgattcc tgcccggcga gggcttcgtc gagggtctcg taggtgtcga
    10021 acagaatcac gagacgggtg gtcagcatga ggttcttgat gcggccgccg acgctcgcca
    10081 gcgcgaacgc agccccgtgg ctgacgcacg cgagccgggc ccgctgcaaa ccggccagtc
    10141 ccatactatt gacccaggag atcttcccga aatcgatgac caccttggtc accccaccgg
    10201 cggtgatctc ctcgacggca tcggtcaagg cacgcgtttc ctctccaccc gtcagattcc
    10261 tcttcggggt caagacgcac acgtcgttca tcatggagcg ctcgaacatc atgcaaaatc
    10321 tccttcggcc ggatggcccc gggtgcaaat acccatatga tcccccgggg cgggggttgc
    10381 atcagacctt gtggctgtaa taccgcggaa ccgcctcgaa agttgccgac aaaattctcg
    10441 cgacgatctt cacgaagcgc cccggcgcgc gtggcttgcg gtaaccgatg ggccgcgcta
    10501 cgcttcgtcc catgatggac acgacaacca tcgactcgaa atcgaacgtc ggcgcgggca
    10561 tatggcggct ggccgacccc aagatatcgc tcgcgtcgat tgcctccatg acgctcgccg
    10621 ccggcgcggc ggtgcgggcg ggcgaggtgt cgatggtgtg gctcgtgctc accgtgctcg
    10681 gaatcctcgc catcgaagtc gcgaagaacg cctcgggtga agtggtcgac tgggactcgg
    10741 gcaccgacgc cgcgattcgg tccgacgagc gcaccccgtt ctcgggcggc aagcgcgtga
    10801 tcgtcgacgg gctgttgacg cgcgcgcaga ccgtgcgcgt cgcggcggtg tcgtacgcga
    10861 tcggcatcgc gatcggtgcg gtgatcgcga tggtgcgcga gccgcgcgtg tggtacttcg
    10921 gtgtcgtggg ggtggcgtgc gcgttcttct accacgccgc gccgctgcga ctttcgtatc
    10981 gcgggtgggg cgaagccgcg gtcgcgttct cgtacggccc cttgatcgcg tgcggcacct
    11041 tcctcgtgca gcaccaccgc atcggcggcc ccgtcgttcc gctgtcgatt ctactgggcg
    11101 tgctgatcgc cgcgttcttg tgggtgaacg aattccccga cgcgcgcgcg gatgcgcagg
    11161 cgggcaagcg caccttggtc gtcaggctgg ggaaggcgcg cgcggcgcgc atcttcgcga
    11221 ttcttgcggt gggtccgttc gccgtactcc cggccctggt agcaaccgga ctcccgggcg
    11281 gcgtgtggct cgcgctggtc ggcctcctcc ccgccctcaa cgcggcccgc atcgtccact
    11341 ccgaaccgga gcaccacgcc cgcatcgccg ccgcgcaggc gagcacgctc gtcgcgttcc
    11401 tactcttcgc gctgggcggc gcgatcggtt tggcggcatg gcggtagcgc cgcagcgcct
    11461 cacacgtagc tttgatattc ggggtcgaag acctgctcgc ggatgaatcc cttcaagtcg
    11521 cggggctcgg ccgtgcccgc gagtccgcgt tcgaaggcga cgcgcgccac cgccgccgcg
    11581 atctccagag aaaccccccg gagcgacggc agcggcggaa acagcagtcc gttcgcgaga
    11641 tcgtccttcg aaaccagccc ggcgagcgtg cgcgccgcgc gcgcgaacat ttcgtcggtg
    11701 acgcgcgcga tgcgtgaggc gacgattccg agcgccacgc cggggaacac gtacgcgttg
    11761 ttcgcctggc tcgggatcat ctgcttcccc tggaaggtga cggtcgcgaa ggggcttccg
    11821 ctcgcgaaca gcgcgcgtcc gccggtgtgc gtgtaggcct cttcggcggt gcattcggag
    11881 ttggcggtcg ggttcgacag agcgaatacg atagggcgct cattgagctt cgccatcgct
    11941 tcgagtatct cgcgcgtgaa ggtcgacggc tggccgctga cgccgatcaa ggccgtgggc
    12001 tcgagcgcgc ggatcgcgtc cagcaggttc gccgcacccg gggcctcgtg cgcgtagggc
    12061 cgcttgtgct ccgccaggtc cttgcggctt cgcaccacga gtccgtggga atcgaccagc
    12121 cagcacgcgc gccgcgcgtc ctcgcgcgac attcccgcgt ccaccatggc cgacacgatc
    12181 agatcggcga tgcccacgcc cgcctcgccc gcgcccagga acaagaagcg ctggcggcgc
    12241 aaatcgccgc cggtgacgcg cagcgccgag aacacccccg ccagcgtcac cgccgcggtg
    12301 ccctgaatgt cgtcgttgaa cgagcacacg cgctcgcggt acttcgcaag caggcgaaat
    12361 gcgttctggt tgccgaagtc ctcgaactgg atcagcacgc cgggccacag ctcgttggcc
    12421 gcggtgacga attcctcgac cagcgcgtcg tagggagcgc cgcgcatgcg gtgccggcgc
    12481 aatccgagat agagcggcgc gtccaacagc gactcgttat cggtgcccac gtcgagtgtc
    12541 accggcagcg tgacggccgg gtgaaccccc gcgcacgcgg tgtagagcgc gagcttgccc
    12601 accggaatgc ccatcccatt ggcgcccagg tcgcccagcc cgaggatgcg ctcgccgtcg
    12661 gtgacgacga tcgcgcgcac gtcggcgtgc ggccagttgc tcaacacgga cttcacgcgg
    12721 tcgcggtcgt ccgcggtgac gaacagcccg cgcgcgcggc gaaagatctg cccgtaaacc
    12781 tggcacgcgc gaccgacggt cggcgtgtag atgatcggca gcaactcttc gatgtgatcg
    12841 acgaccacgc ggtagaacag cgtctcgttg cgatcgtgga gcgacgtcag atagatgtac
    12901 ctctcgaggt cgttgggctt ggcgcggtag ttggtcatca cgcgcgcgat ctgctcgttc
    12961 atgtcggcgg ggcgcggcgg caacagaccg cgcagcccca gggcgtcgcg ctcttcgggc
    13021 gtgaatgcgg tgcctttgtt gagcagcggg tcgctcaaga gatcgactcc gcgcgggaaa
    13081 tcgctggggc gcgtgcgcgc gccggtggga cgcttgctca ttattgaact gactccgatc
    13141 tgcggcgggt ttcgaaaagc cagcagatcg tccctaattc gcgccgatgt gtcaagcgct
    13201 cccgcgaacg caagtgcgtt cgcggggcgc ttgcccgctc gcgcgtgctc ctatttgacg
    13261 agcaccatct tgcgcgccac cgtccgcttc cccgccgcga gcgtgtagaa gtacgtcccc
    13321 gaagccaccg gcgtgcccgc gtcgtcgacg ccgttccact ccacgtggtg ccgtcctgca
    13381 cccaggggcg agtccagcac ggtcgccacg tgcgacccac gcacgtcgta gatgcgaaga
    13441 atcgcgcgtg cggattccgg cagcgtaaac ccgatgcgtg tcgtgggatt gaagggattg
    13501 gggacgttgg gttcgaggtc gaaggagagc ggcgccacca tcgccaccac cggaatcgag
    13561 aacacctcct cgccattctc cttgaccgcg gccagcacgt acgtgtacgc gacccctccc
    13621 cgcacactcg cgtcgacgaa cgacgtcgcg tcgctgggga ggagcgcatc gttcagtacg
    13681 cgctgcgcct cgccggcggc gccgcgatac acgcggtacc cgcttacgac ctcatcggct
    13741 tcgacctgcc actccaagtg aaccccgtcg ccccgcggcc gcgcgtcgaa gcgcgaaatc
    13801 aacaccgaga cgatcacgat gtcgaccgat cgctcgaccg agtatgcgct cagcatcccg
    13861 ccgatgttct tcgcgcgtac ggcgaagtac tgccggccga gcggaaggaa gcacacctcg
    13921 gcggagccgg tcgctcccac ttcgatcatg ttttcgtagt tcgaggcgcc gcccccggcg
    13981 accgactggc ttccgtaggc cacgacgtat ccgatgaccg tgggatcgcc gctttgcatc
    14041 cacgacgcgg tcgcgcaccc gggcagaccc acctcgaccg atgtgaatag cggtggcgcc
    14101 ggcggcgcca gatcgccgac gggcgcgatg gctacgaggg tgcccgtgga gacgtccatc
    14161 acgaacgcca gcgatccgag agccgtcgac gcccccgcgg aaacgggaac cccgttccga
    14221 atgaggtcga atgaagtagc gggcttgaca ccgtacatga gcacggtcgc gtcgaccgcg
    14281 cacgcgggcc gcgggtggcc gagcgccgcc cacgagagca cgaccaccgt ggccgcgcgt
    14341 gcaagtccaa aggaacgaac gcagactgat ccggatgcat ggccgatgcg ctgcatggag
    14401 gtgctccttc ggcggcgacg gccgcggtgg ttgcgcgcgc atcgacgctg gtgaggtttc
    14461 cgttcttatt gaatccaggg attgaggcgt ccccatgcag caaattccgt gcagcgaaat
    14521 gggtgcggtg cttcggcgcg gcgcagcgtg gtgcagcacg gcgcatgcgc gcacgatgct
    14581 cttcat
//
LOCUS       DASPGK010000069         8890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1071382, whole genome shotgun sequence.
ACCESSION   DASPGK010000069 DASPGK010000000
VERSION     DASPGK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071382"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VEC56_01840"
     CDS             <1..713
                     /locus_tag="VEC56_01840"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXV12925.1"
                     /translation="FRDRFGMTFHAYCRARRMAGALHDIRVGLDLDAVVFDHGYESHS
                     GFRSAFAKTFGRPPGRSRATECIEVALVETPLGPVVVGATASALCLVEFATRRMLASE
                     VGVLRRRFGAVVVPGHNAVIAQAARELDEYFAGRRTRFDVAVAAPGTPFQERVWAAVR
                     AIPYGATESYEAIARAVGRPGAVRAVGTANGANRLAIVIPCHRVRKKSGELGGYGGGR
                     WRKEALLDLERSRSPSRT"
     gene            836..1495
                     /locus_tag="VEC56_01845"
     CDS             836..1495
                     /locus_tag="VEC56_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV12926.1"
                     /translation="MRDSSRPASGGVVGLCLALALAAIAGSCYPGDQLTIADSDVIVT
                     LYDPGADFSSKQSYAMPDTIIHLVEDGGTDTISRAYDDEVLARVASNMGGLGFTRETD
                     PNVADVFMLISVAVNDQVGYTGYPWGGYWGWYYPYPPGWGWGYYPWYGGGGTVYTYRT
                     GSVFLQMVDPAAADTVQTRVPTIWIGALNGLVQGDAVEERILNGIDQAFAQSPYLGAG
                     K"
     gene            1502..2125
                     /locus_tag="VEC56_01850"
     CDS             1502..2125
                     /locus_tag="VEC56_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12927.1"
                     /translation="MRHRSAKILTASAVVAALVGALVPAARAQDWFGVATWQVSFPID
                     DTQLFTDEVSFRGAGIDFRKTLTGGTVASITMAWNVFHERTGGVYELEAGAISGTQDR
                     YINSFPIMVGVHQYFGNRRSTRAYVGLNAGGYLLIQTFRLGITEIEEDSWEWGASPEA
                     GVVIPIQTGAWFVVNAHYHWSPTPESLAGNEVSLTYYQLNVGFMWEQ"
     gene            2346..2912
                     /locus_tag="VEC56_01855"
     CDS             2346..2912
                     /locus_tag="VEC56_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXV12928.1"
                     /translation="MIIQCQSCAAKYFLSEEKVPANPLKVRCPKCKAVFMLAPRSERV
                     PVGAGVAAAAPAPAAVPLDIETTASPRKKAAAAVPLDVTEDSPARAQAAAPAPAPAAP
                     AKSGRGGKGEDRAKRLARVLVSDILYYNRERRDQALREGNLMTALSEEIKKSWELYKE
                     KVGPEAAQSTSYFKEALNEILADGQEVF"
     gene            2947..3816
                     /locus_tag="VEC56_01860"
     CDS             2947..3816
                     /locus_tag="VEC56_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV12929.1"
                     /translation="MRSDRTVFVVAADDAYLEHVFASCADPPASAARFATIEALCHAL
                     AQFERLSLAFVLLVDARGDRIDIPALRRLRLDFPQVVLMALLGECDQQSALRLQSIGV
                     HGILLPPFERVNLGREITTAVPNVPNFKRHPDLMRRGSVRLDFVIPSDLTYVLGINYH
                     ISNLLKEFGYPPQDTRVNVPLACDEAITNAIVHGNRSRADKKVSIQIYASSNRFRIRV
                     RDEGEGFDLGRVANPTQGEALLRASGRGVYLMRSIMDSVEFKDGGRVVELEKRNANAV
                     GNGSDTENGGTRA"
     gene            complement(3818..4276)
                     /locus_tag="VEC56_01865"
     CDS             complement(3818..4276)
                     /locus_tag="VEC56_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12930.1"
                     /translation="MKNRNTSSGIVEKMMKEMFARLLNIEVSQSVIEKLVPVIEGVTE
                     KIDGQIDLDTVQGQNTFRALICSMVAHGWGLGMHPDNNPSVNLDSKALDVKVCKDILV
                     PMETLKSIRYVKKDQYGRVRKALFEPDQNARIWGSIDEMLEKLAKEKWQE"
     gene            complement(4279..5514)
                     /locus_tag="VEC56_01870"
     CDS             complement(4279..5514)
                     /locus_tag="VEC56_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV12931.1"
                     /translation="MAAIRRDVSLPFLSAVQFVALSADRLKQFSLVGMLGLLSPGSSV
                     DLLKLTLFSQVPILLFTPLAGVLIDRWNKALTILAACVVRAVILIALPSAYARTQSLD
                     TFFVAAGTLAFFDLMFAPARSALLPEVVSKERLLGANATFWTLGIAGTLLGFVGGGWL
                     FEAWSWQMSFHVDALLYVAAAALMLPLVFRERPPRTAPLARASMREAALIMLHSVRDA
                     VVLVRESRELRASLGAQAALFAVGGILSILAIARIQEVALDGSARFLSQVAAAFIGGL
                     IVGAFVAIPFRDTRLPERTVSVGTVVAGVAIAGLGRTETLIPMCVWSALLGAAISPVF
                     VVTETLMQRVSPRQFTGRVFAAREAAIKAAYIGAAVLATGLDAVLSRTALLVGLGLFL
                     ALLGVILERTRWSRSEKSE"
     gene            5869..6429
                     /locus_tag="VEC56_01875"
                     /pseudo
     CDS             5869..6429
                     /locus_tag="VEC56_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
     gene            7579..8682
                     /locus_tag="VEC56_01880"
     CDS             7579..8682
                     /locus_tag="VEC56_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12932.1"
                     /translation="MRKENSARIPLLRRRGVETEVDPQAEFERARLAWDAGRRREALD
                     AIERIARRDAVPASLLVAIGQFMTDAGELFVAAECFERLANEDSERALPHYFLGRIYD
                     RLGRLDDAVRAHKKAVELEPSNAECHYRMGVTFARNERRPEAEECYRRAIALDRGHAR
                     AYTNLGYLMDLRGENAQAIQFFRRALQFNPQSAEAHFNLGALYGEHGSHEEAIREFRQ
                     AIKLAPESVEAHYNLGLALYGFGDMEGALEAFRRVLKLESGHQQAPFFIGMIYYRKGL
                     YAKAVKLLQSALKASPDHPSILYHIGMCYNHLNQPVRAIPPLKRVTELTPEDHRAFYA
                     LGMVYDRADRQDDARECFRTADAKLHAHRQRVA"
     gene            8679..>8890
                     /locus_tag="VEC56_01885"
     CDS             8679..>8890
                     /locus_tag="VEC56_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12933.1"
                     /translation="MSAGRVVRPDTRFFLTLAWILSVVPVVTHAVGHERIPLDSWSYR
                     AVERFEALGYCTLPEDRPYSRDEFIR"
BASE COUNT         1550 a         2985 c         2930 g         1425 t
ORIGIN      
        1 acttccgcga ccgcttcggc atgacgttcc acgcctactg ccgggcccgg cgtatggcgg
       61 gcgcgctcca cgacatccgc gtgggcctcg atctcgatgc ggtcgtgttc gaccacggct
      121 acgagtcgca cagcggattc cgttccgcct ttgcgaaaac gttcgggcgt ccgccgggcc
      181 gttcgcgcgc gaccgagtgc atcgaggtgg cgctggtgga gacgccgctg ggaccggtcg
      241 tcgtgggagc gacggcgtcg gcgttgtgcc tggtcgagtt cgccacgcgg cgcatgcttg
      301 ccagcgaggt cggcgtgctt cgacgccgct ttggggcggt cgtggtgccg ggtcacaacg
      361 cggtgatcgc gcaggcggcg agggaactcg acgagtactt cgcgggacgg cgcacccggt
      421 tcgacgtcgc cgtcgccgcg ccgggcacac cgttccagga acgcgtgtgg gccgcggtgc
      481 gggcgatccc gtacggcgcc accgagtcgt atgaggcgat cgcgcgcgcg gtcggccggc
      541 cgggtgcggt gcgggcggtg ggcacggcca acggcgccaa tcgcctcgcg atcgtcattc
      601 cgtgccaccg cgtgcgcaag aagtcgggcg agctcggggg ctacggcggc ggccgctggc
      661 gcaaggaggc gctgctcgat ctggagcgaa gccgctcgcc ctcgaggacc tgagagcgga
      721 gggagccctc tagacgagcc cgagcccatc gtgttttttc ttggtgactt ccgcaccctt
      781 cccgtacatt tcccctcgac gcccgggccg gaaatcgtga aaggaaccaa ccatcatgcg
      841 tgattcatcg cgacccgcga gcgggggagt cgtcggactg tgcctcgcgc tcgcgctggc
      901 cgccatcgcc ggctcgtgct accccggcga ccagctcacg atcgcagact ccgacgtcat
      961 cgtgacgttg tacgaccccg gcgccgattt ctcgtcgaag cagtcctacg cgatgcccga
     1021 cacgatcatc cacctggtgg aagacggcgg caccgacacc atctcgcgcg cctacgacga
     1081 cgaggtgctc gcgcgcgtcg cgtcgaacat gggcggcctc ggtttcacgc gcgagaccga
     1141 tcccaatgtg gcggacgtgt tcatgctgat ttcggtcgcg gtcaacgacc aggtgggtta
     1201 caccggatat ccgtggggcg gttactgggg ctggtattac ccgtatccgc cgggctgggg
     1261 ctggggctat tacccgtggt acgggggcgg tggaaccgtg tacacgtatc gcaccggctc
     1321 cgtgttcctg caaatggtcg atcccgcggc cgccgacacg gtccagaccc gggtgccgac
     1381 catctggatc ggcgcgttga acggcctggt ccaaggcgac gcggtcgaag aacgcatctt
     1441 gaacggcatc gatcaagcct tcgcgcaatc gccgtatctc ggcgcgggaa agtagcgccg
     1501 catgcgacac aggagcgcga agattctcac cgcgtccgcg gtcgtcgcgg cactcgtcgg
     1561 tgcactggtt cccgccgcgc gcgcgcagga ttggttcggc gtggcgacgt ggcaggtctc
     1621 gtttcccatc gacgacaccc agctcttcac cgacgaagtg agcttccgcg gcgccggcat
     1681 cgattttcga aagacgctga cggggggcac ggtcgcgagc atcacgatgg cgtggaacgt
     1741 cttccacgag cgcacgggcg gcgtttacga gctcgaggcc ggggccatct cgggcacgca
     1801 ggaccggtac atcaactcgt ttccaattat ggtgggcgtg caccagtatt tcggcaatcg
     1861 acgctcgacg cgcgcctacg tcggcctgaa cgccggtggc tacctgttga tccagacgtt
     1921 tcgactcggc atcaccgaga tcgaggaaga ctcgtgggag tggggcgcgt cgccggaagc
     1981 cggggtcgtg attcccatcc aaaccggggc gtggttcgtc gtcaatgcgc actaccattg
     2041 gtcgccgacg ccggagtcct tggccggcaa cgaggtatcg ttgacgtact accagctcaa
     2101 cgtgggcttc atgtgggagc agtagggccg gagtccggcg tgaggcgggc gtttttgccg
     2161 tgcggcggat tcccgcagcg ggtcgaggcg cggcaggtcc ttcctggcgt gatagttgct
     2221 cccaaggccg gggtgcccct cggcgaagtc cgccgagacg tcctgcggcc cacccgggaa
     2281 catcgatcgg tgcgcggggg gaccgtgaag gcaccgcggc cagcctcccg acggagtgat
     2341 tcgcgatgat catccagtgc caaagttgcg ctgcaaaata cttcctgtcc gaggaaaagg
     2401 tcccggccaa cccgctcaaa gtgcgctgtc cgaagtgcaa agcggtgttc atgctggcac
     2461 ctcgcagcga gcgcgtcccg gtcggtgccg gtgtcgcggc ggccgccccc gcccctgcgg
     2521 cagtgcctct cgacatcgag acgacggcct cgccgcgcaa gaaggccgcg gccgccgtgc
     2581 ctctggacgt aaccgaagac tcgccggcgc gcgcacaagc ggccgctccg gctcccgcgc
     2641 ccgcggcgcc ggccaagtcc ggtcgcggcg gcaagggcga ggatcgcgcc aaacgactgg
     2701 cgcgcgtgct cgtctccgac atcctgtact acaatcgcga gcgtcgtgat caggcgctgc
     2761 gcgaaggcaa tctcatgacc gcactgagcg aagagatcaa gaaatcatgg gagctctaca
     2821 aggaaaaggt cggccccgag gccgcgcaat ccaccagcta cttcaaggaa gcgctcaacg
     2881 agatattggc cgatggccaa gaagtcttct gattccattc ccggcttacc gccggtcgca
     2941 ccgtcgatga gaagcgatcg aacggttttc gtcgtcgcgg ccgacgacgc ctatctggag
     3001 cacgtcttcg cctcctgcgc ggacccgccg gcttcggcgg cacgtttcgc caccatcgaa
     3061 gcgctttgtc acgcgctcgc gcagttcgag cgcctctcgc tggcgttcgt cctcctggtc
     3121 gacgcgcgcg gcgatcgcat cgacatcccg gcgctgcgcc ggctccggct ggattttccc
     3181 caggtcgtgc tgatggcgct cttgggtgag tgcgaccagc agagcgcgct gcgtttgcag
     3241 tcgatcggtg tgcacggcat cctcttgccg ccgttcgagc gcgtcaactt gggacgcgag
     3301 atcacgaccg cggttcccaa cgtccccaac ttcaagcgcc accccgacct catgcggcgc
     3361 ggaagcgtgc gcctggactt cgtgatcccc agcgacctca cctacgtgct cggcatcaac
     3421 taccacatct cgaacttgct caaggaattc ggctacccgc cgcaagacac gcgcgtgaac
     3481 gtcccgctcg cgtgcgacga agccatcacc aacgccatcg tgcacggcaa tcggtcgcgc
     3541 gcggacaaga aggtgagcat tcaaatctac gcgagttcca accggtttcg catccgcgtg
     3601 cgtgacgaag gagagggctt cgacctgggg agggtcgcca acccgacgca gggcgaggcg
     3661 ctcctgcgcg cgtcggggcg cggggtctac ctgatgcgca gcatcatgga ctcggtcgag
     3721 ttcaaggatg gcggccgggt ggtggaattg gagaagcgca acgcaaacgc ggtcgggaac
     3781 ggaagcgaca ccgagaacgg cggcacgcgg gcctgagtta ctcctgccac ttttccttcg
     3841 cgagcttctc cagcatctcg tcgatggatc cccagatgcg cgcgttctga tcgggctcga
     3901 agagcgcctt gcgtacgcgc ccgtactggt ccttcttgac gtagcggatc gacttcaggg
     3961 tctccatcgg gaccaggatg tccttgcaca ccttgacgtc gagcgccttg gagtcgaggt
     4021 tgacgctggg gttgttgtcg gggtgcatgc cgaggcccca cccgtgcgcc accatgctgc
     4081 aaatcagggc ccggaaggtg ttttggccct gcacagtgtc gaggtcgatc tggccgtcga
     4141 tcttctcggt cacgccctcg atgaccggga ccagcttctc gatcacgctc tggctcactt
     4201 cgatgttgag caaccgggca aacatctcct tcatcatctt ctcgacgatg cccgatgagg
     4261 tgttgcggtt cttcatgatt actccgattt ctccgatctc gaccaacgcg tgcgttcgag
     4321 gatgaccccc agcaaagcaa gaaataagcc caaccccacc aggagtgcgg ttcgggacag
     4381 taccgcgtcg agtccggtcg ccagtaccgc cgccccgatg taggccgcct tgatggcggc
     4441 ctcgcgcgcc gcgaacacgc gcccggtgaa ctggcgcggg ctcacgcgct gcatcaaggt
     4501 ctcggtcacg acgaacacgg gggagatcgc cgcgcccagg agcgcggacc acacgcacat
     4561 cgggatcagc gtctcggtgc gcccgaggcc ggcgatcgcg actcccgcca ccaccgttcc
     4621 caccgaaacc gtccgctccg gcagccgcgt gtcgcgaaag ggaatcgcca cgaaggcccc
     4681 gacgatgagc cccccgatga acgccgccgc gacctggctc aagaagcgcg cgcttccgtc
     4741 cagcgccacc tcctggatgc gcgcgatggc gagaatgctc aagatgccgc ccaccgcgaa
     4801 gagcgccgcc tgcgcgccca ggctcgcgcg cagctcgcgg ctctcgcgta ccagcacgac
     4861 cgcgtcgcgg accgaatgca gcatgatcaa cgcggcctcg cgcatggacg cgcgcgcgag
     4921 cggtgctgtg cggggcggtc gttcccgaaa cacgagcggg agcatgagag ccgccgcggc
     4981 gacgtagagc agcgcatcga cgtggaagct catctgccac gaccaggctt cgaacagcca
     5041 gccgcccccg acgaagccga gcagcgttcc tgcgatcccc agggtccaga aggtcgcgtt
     5101 ggcacccagc agtctttcct tcgacaccac ctcgggcagg agcgcggaac gagccggcgc
     5161 gaacatcaag tcgaagaatg cgagcgtgcc cgccgcgacg aagaacgtgt cgagcgattg
     5221 cgtacgcgcg taggcgctcg gcagcgcgat cagaatcacg gcgcgtacca cgcacgcggc
     5281 gagaatcgtg agcgccttgt tccagcgatc gatcagcacg cccgccagcg gagtgaacag
     5341 gaggatgggg acttgcgaga acagcgtcag cttgagcaga tcgacgctcg agccggggga
     5401 tagcaggccg agcattccga ccagggagaa ctgcttcaac cgatcggccg acagtgccac
     5461 gaactgcacg gcgctgagga aggggagaga gacgtcgcgg cgaatcgctg ccatcgaagc
     5521 tggcgcctat cctaggcgcc gggtcgcgct tttgcaagtc ccggagcata ttgcagccgc
     5581 gtcctgctcg aaacctcccg cggccgcccc gagcggcgtc aagttgcgtg gctcaaacgg
     5641 gttgggcgtt ccgcacggct cctcggttgg catgctactt gcccgctagc cggctgccgg
     5701 ggcggtccgt tcgcagaagc gcttgagctt tctcaccctg gcctcatcag caagagcacg
     5761 atctgtgggc cgccccggca gaactcacgc ccgacttggg cgacgtcaat caaccctgcg
     5821 ccgtcggcga acgccgcgcg cgcacacgag acggaaggac gcgacgacat ggtcgtgctg
     5881 aactataccg gcggggagat caacgccaag atcgtctact acggccccgg cgtgagtgga
     5941 aagacgacca atctggaatt catccacgag aagacgccga acaatgccaa gggcaagatg
     6001 gtctcgatga agacgcagac cgaccggacg ctcttctttg acttccttcc cctcgagctg
     6061 ggcgagatca acggctaccg cacgcgcttc ctgctctaca cggtgcccgg ccaggtctac
     6121 tacaacgcga cgcgcaagct ggtcttgaag ggcgtcgacg cggtcgtgtt cgtcgccgac
     6181 tcctcgcgcg cgaagctcga ggagaatctc gagagtctgc gcaacctcga acagaacttg
     6241 aacgagcacg ggatgacgct ggacgagatc ccgtgggtga tccagtacaa caagcgcgac
     6301 ctcccggacg cggttccgct cgccgagctc gacgcgcgcc tcaatccgct caagaagccg
     6361 tcgttcgaag cggtcgcgag caagggcacg ggcgtgtacg agaccttcca cggcatcgcg
     6421 accatggtgt accgcgtgct gaaggaccgg ctcgagaagg gcgacttgtc gaccgaggtc
     6481 ggcgacgccc cgccggccga gacgacgcgc ccaggcgcaa agccacccgc gggacgcggc
     6541 aagggcaaag cgatcgtggc cgatatcgtg atcgagccca ccgccctgcc acgcgcggca
     6601 cgcccggcaa caccgaccgc gcccaagccg gcgccgccgg agcccgccga tcccccggcg
     6661 gccgacccat tcgaatacga cgaccacgtc gacttggtgc tgcgcgaggt cgatccacct
     6721 gcggcaacac gccaagccgc gaagccgggc gctccggccc ccaagccgag cgcgcgcaaa
     6781 cccgcgccgg agccggtgcc tgcgggaccc gcgaagacgc cggggcgtcg tcccgccgaa
     6841 gcgcccgccg ccaaagcggt cgggcacggc gatgcaccgg tggacgagaa tctcttcgcc
     6901 gcgattccgg agtcaccgtt tgacatggag ctcggccgct tgccggctct cgacgaagca
     6961 tcgctcgcgt cggaaagcgc gcgcgaagaa ggtccggacg agttcgtcac cgatcccatg
     7021 cgccgcgtcg aacccaagcc ggcgccgccg aaagcgcccg aaccggcgcg cgaggtccgt
     7081 cccaaagtcg acgcgcggcc cgcgaagacc gaaccgcgtc cgccgatcga agagcgcgtg
     7141 gcggtcgagg cgcacgtcac ccaggaaatt cgcgaggtac gcgtgatggt gcctctcatc
     7201 gtcacgcgcg cgcagctcga aggcggggcg ccgattcgcg tcgtcttcga cttgaagatc
     7261 gtcgactgat ccaccccgcg gcgaacaacg ggcgtccccc ggcgaacgct gcgcggcggg
     7321 gggcccatcg gcgccacccg ccgcgggacg tccagaggcc cgccagttcc cccgctccct
     7381 ctcccgaaca tttctgtgga taaggaaccg aaccactttg tgagaaagca cttccgcgtt
     7441 tggggaaaag tcttgacagt ttttcgatag gtcacttacg gtgacgcccg aagtcaatgc
     7501 atctcgttga ctcggcgtga attatcgcgt ttggagcggt ccgccgaggg tcttctcgag
     7561 gaggcgggcg tgctgcgcat gcggaaggaa aactcggctc ggatccccct gttgcgccgg
     7621 cgcggggtgg agacggaggt cgacccccag gccgagttcg agcgggcgcg cctggcctgg
     7681 gacgccgggc ggcgccgcga ggcgctggac gccatcgagc gcattgcacg gcgcgacgcg
     7741 gtgcccgcgt cgctcttggt cgcgatcggt caattcatga ccgacgcggg cgagttgttc
     7801 gtcgcggccg agtgcttcga gcgcctggcg aacgaggatt ccgagcgtgc cttgccgcac
     7861 tacttcctcg ggcgcatcta cgaccgcctc gggcggctcg acgacgcagt gcgagcgcac
     7921 aagaaggccg tcgagttgga gccgtcgaac gcggagtgcc actatcgcat gggtgtgacg
     7981 ttcgcgcgca acgagcgccg ccccgaggcc gaagaatgct accgccgcgc gatcgcgctg
     8041 gaccgcggac acgcgcgcgc ctacaccaac ctcggttact tgatggacct gcgcggcgag
     8101 aatgcgcagg cgattcagtt ctttcggcgc gcgctccagt tcaatccgca gagcgccgag
     8161 gcacatttca atctgggggc gctctacggc gagcacggca gccacgagga agcgattcgc
     8221 gagttccggc aggccatcaa gctcgcgccg gagagcgtgg aggcgcacta caacttgggc
     8281 ctcgcgttgt acggcttcgg cgatatggaa ggcgcgttgg aggcgttccg gcgcgttctc
     8341 aagctggaat cggggcacca gcaggcgccc ttcttcatcg ggatgatcta ctatcgcaag
     8401 gggctgtacg cgaaggcggt caagctcctg cagtccgcgc tcaaggcgag ccccgaccat
     8461 ccgtcgatcc tgtatcacat cggcatgtgc tacaaccatt tgaaccagcc cgtgcgcgcg
     8521 atcccgccct tgaagcgcgt gacggagctg acgcccgaag accaccgcgc gttctacgcg
     8581 ctcggcatgg tgtacgatcg cgccgatcgc caggacgatg cgcgcgagtg tttccgcacc
     8641 gccgacgcca aactgcacgc ccaccggcaa cgcgtcgcat gagcgccggt cgcgtcgtgc
     8701 gacccgacac gcgcttcttt ctcacgctcg cgtggatact ctcggtcgtc ccggtcgtaa
     8761 cccacgcggt cggtcacgaa cgcatcccgc tggatagttg gagctaccgc gcggtcgagc
     8821 gcttcgaagc actgggctac tgcacgctcc ccgaagaccg gccgtattcg cgcgacgagt
     8881 tcattcgaat
//
LOCUS       DASPGK010000070         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_252627, whole genome shotgun sequence.
ACCESSION   DASPGK010000070 DASPGK010000000
VERSION     DASPGK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252627"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..727)
                     /locus_tag="VEC56_01890"
     CDS             complement(20..727)
                     /locus_tag="VEC56_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12934.1"
                     /translation="MRPVLLTITLLLAATVALAGPPQTGVYLSNDFGGTMLPGRFSES
                     WVNPLASHGQIGNTIHALSWDGLTLGDEWRLLCPSIAAAPVLVSDNRDGNGTGDVTYQ
                     TQYGGGTFWMSMNGPWGDGTVDYTGVIQTFVVTTTFQYVFGNVLGIRGNVTMSGSFDG
                     YDNCFDYTVNNTAIVGTTDTMLLPADYPAFRDASCFVGPTRGSWGSVTQIALQIYGSC
                     TVTTEESTWGAVKALYR"
     gene            935..3064
                     /locus_tag="VEC56_01895"
     CDS             935..3064
                     /locus_tag="VEC56_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV12935.1"
                     /translation="MRRAGVGADRILIGCVVVIAIATAVLHAVGARPNLSWMWGVHFY
                     VFYPLWVLVVATAALAAVVLTTIVRPKALADAVTNALRPALEGRLHLAFAAACIVAGA
                     LLFWFARISHTYLGDGNIIVEEIDTSRQLLEREPLSSLLQYAVYRTTKSWFHDPNRSI
                     ELNAQDALAPGSVLAGFFFLMVAWFLASELARLVKTDEDRHTRVITALVWLTVVAQGY
                     VQLFFGYIENYSFYAVGVVAYLWLALRHLRGAAPLLFPALALILCFALHLSSLVLGAS
                     FVVLAACALAHRESRLRALRDLGVAIAICFAVVFAFSRVREGYNPLMTLLGMVKTAFA
                     HESSRAYMFSSQHYRDFFNEQLLIGPLGMFLFLPAAAAAVRAKDARRHPAAWFVLVAG
                     LGFLVACWLIGDSNLGYARDWDLLSHSGLVFTTASLVLLLLPRVRHTVVVSALVCAAA
                     TSLYHTTPWIAVNADEPRSVARLQTLPLGLGRTEVVVSTYYRQRGDTENQRVWLQRAL
                     VTNPNNVNAIYLLGALDLKSGRYEDAVVSLEQAVRMRPDKVMFRTALVQALSALNRLQ
                     EAIPHLEMLARNEGNSLLVQITLGEALRGTGRIAEAQRAFEQAERLCRPIVDANPGDP
                     RITVAHGFTLMRLGRLDEAERQLTRAIALDPNLADAHCFLGYVMRDQGRPREATQEFD
                     TCLALNPAFGGRLEIEEWLSHNRPRTP"
     gene            complement(3079..4059)
                     /locus_tag="VEC56_01900"
     CDS             complement(3079..4059)
                     /locus_tag="VEC56_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12936.1"
                     /translation="MTTKRLFAIGPWVSATCLAAALACQPDVYPPVEQSKQLVTADPV
                     DPKVIVFVIDGPRYQDSFGDSLHAHVDEIWSQLRPLGTLCSNYRNLGWTLTIPGHATM
                     LTGVWQYLDNEGLERPQEPTLFEYYRKATGAPATDAILVGGKPKLAACAYSNDAAHGA
                     AFGASTDLDNTTDLATYDALIQRLQTDRPHLAMASFSQVDQKAHGGSWSAYLRQLEIV
                     DSLAVLTWNYLQADAEYANQTYLFITADHGRHDDAHGGFQNHGDACDGCQHVIFMALG
                     PDIRSDYEVTTLYTQRDVCTTVGQLLGVAAPQSDGFAMEEIFQPVSTGVR"
BASE COUNT          707 a         1368 c         1325 g          803 t
ORIGIN      
        1 ccgacgactt acggttccat tatcggtaaa gtgccttgac cgcaccccaa gtcgactctt
       61 ccgtcgtaac ggtgcacgag ccgtaaatct ggagcgcaat ctgtgtgacc gatccccaac
      121 ttccccgcgt cggccctacg aagcagctgg cgtcgcgaaa cgccgggtag tccgccggga
      181 gcaacatcgt gtcggtggtg ccgacgatcg cggtattgtt gacggtgtag tcgaagcaat
      241 tgtcgtagcc gtcgaacgaa ccactcatcg tcacgttgcc ccgaataccg agaacgtttc
      301 caaagacgta ctggaacgtg gtcgtcacga cgaaggtctg aatgacgccg gtgtaatcca
      361 cggtgccgtc accccacggc ccgttcatcg acatccagaa cgtgccgccg ccatactgcg
      421 tttggtaggt cacgtcgccg gtgccgttgc cgtcgcggtt atcggagacg agcacaggcg
      481 cagccgcgat cgacgggcac aagagcctcc actcgtcgcc gagcgtcagg ccatcccacg
      541 agagggcgtg gatcgtgttg ccgatctgcc cgtgtgatgc cagcggattc acccacgact
      601 cgctgaaacg gccgggcagc atggtgccgc cgaaatcatt cgaaagatag acacctgttt
      661 gcggcggacc cgcgagggcg accgtcgcgg ccaggagaag cgttatggtc aatagaactg
      721 gtctcattgg ttgcctcctt cggccaaata gtcctttcga agggagtata gcaatttcag
      781 agaaaattat ctcaaataat cgattgcctc gttcttttcc cctcaaggga ttgtgcttat
      841 gtcggttatg cccatgaccg aaccggcgtc acgggtcgac ttgccaccgg cgggacgcct
      901 ggactagcat cgagttatgc ccgccccgca acgcatgcgt cgggccggag tcggcgccga
      961 ccgcattctg atcgggtgcg tcgtggtgat tgcgatcgcg acggcggtcc tgcacgcggt
     1021 gggggcgcga ccgaacctct cgtggatgtg gggcgtccac ttctacgtct tctatccact
     1081 ctgggttttg gtcgtcgcca cggcggcact cgcggcggtc gtgctcacga ccatcgtccg
     1141 tcccaaagcg ctcgccgatg cggtcacgaa tgcgctgaga cccgcgttgg aaggtaggct
     1201 ccatctcgcc ttcgccgccg cgtgcatcgt cgccggtgct ttgctcttct ggttcgcccg
     1261 catctcgcat acctatctgg gcgacggcaa catcatcgtc gaggagatcg acacctcgcg
     1321 gcaattgctc gagcgcgagc cgctctcgag tctcctgcaa tacgcggtct atcgcaccac
     1381 caagtcgtgg ttccatgacc ccaatcgatc gatcgagctc aacgcccagg acgccctcgc
     1441 gcccggcagc gtcctggcgg gcttcttctt cctgatggtc gcgtggttcc tcgcgagcga
     1501 gctcgcgcgc ctcgtgaaga cggacgagga tcgtcacacg cgggtaatca cggcgctcgt
     1561 gtggctcacc gtcgtggccc aggggtacgt gcagctcttc ttcggctaca tcgagaacta
     1621 ctcgttctac gcggtcggcg tcgtggcgta cctgtggctc gctctgcggc acctgcgcgg
     1681 cgcggcaccg ctgctgttcc cggcactcgc gctgatcctc tgcttcgcgc tgcacctatc
     1741 gtcattggtc ctgggcgcgt ccttcgtcgt gctggctgca tgcgcgctgg cacacagaga
     1801 atcccgcctg cgcgcgttgc gcgacctggg cgtcgcgatc gcgatttgtt tcgccgtcgt
     1861 gttcgccttc tcacgcgtgc gcgagggcta caatcccctc atgacgcttc tggggatggt
     1921 gaagacggcg tttgcccacg agtcgagccg tgcctacatg ttctcgagtc agcactatcg
     1981 cgacttcttc aacgaacagc tgctgatcgg tccgctgggg atgtttctgt tcttgcccgc
     2041 ggcagcagcg gctgtgcgcg ccaaagacgc gcgtcgacac ccggccgcgt ggttcgtcct
     2101 ggtcgccgga ctgggcttct tggtggcgtg ctggttgatc ggcgattcga atctcggata
     2161 cgcgcgcgac tgggatctgc tctcgcactc agggctggta ttcacgacgg cgtctctggt
     2221 tctgctgctg ctgccgcgcg tgcgtcacac ggtcgtggtc tcggcgctcg tgtgcgccgc
     2281 cgcgacgtcg ctctatcaca ccacgccctg gatcgcggtc aacgccgacg agccgcgcag
     2341 cgtcgcgcgc ttacaaaccc tcccgctcgg actgggccgt accgaggtcg tcgtgtcgac
     2401 gtactaccgg cagcgcgggg acacggagaa ccaacgcgtg tggctgcagc gcgcgctggt
     2461 caccaacccc aacaacgtga acgcgatcta cctgctgggg gccctcgatc tcaagtcagg
     2521 gcgctacgag gacgccgtcg tgtcgctcga gcaggcggtt cgaatgcgcc ccgacaaggt
     2581 gatgttccga accgcgctgg tccaggctct gagcgcgctc aaccgactcc aggaggcgat
     2641 tccacacctc gagatgctcg cacgaaacga ggggaacagc ctcctcgtgc agatcacgct
     2701 cggcgaggcg ctacgaggta cggggcggat cgccgaagcg cagcgggcgt tcgagcaagc
     2761 cgagcggctg tgccggccca tcgtcgacgc caacccggga gacccccgca tcaccgttgc
     2821 gcacgggttc acactcatgc ggctcggacg actggacgaa gcggagcgtc agctcacgag
     2881 agcgatcgcg ctggatccga acctcgccga cgcccattgc ttcctgggct acgtgatgcg
     2941 cgaccagggc cgcccgcgcg aagcgaccca ggagtttgac acctgccttg cgctcaaccc
     3001 ggccttcggc ggacgcctgg agatcgagga gtggctctcg cacaaccggc cccgaacacc
     3061 gtgatctcgc caactcgact agcgcacgcc cgtcgaaacc ggctggaaga tttcctccat
     3121 cgcaaatccg tccgactgcg gagcggccac gccgagtagc tggccgacgg tggtgcacac
     3181 gtcgcgctgg gtgtagagcg tcgtcacctc gtagtcggaa cgaatgtcgg gccccagcgc
     3241 catgaagatc acgtgctggc aaccgtcgca cgcgtcgccg tggttctgaa aacctccgtg
     3301 tgcgtcgtcg tgacggccgt ggtcggcggt gatgaaaagg taggtttggt tcgcgtactc
     3361 cgcatcggct tgcagataat tccatgtgag cacggcaagc gagtcgacga tctccaattg
     3421 tcgaaggtac gcactccagc tgccgccgtg cgccttttgg tcgacctgcg agaaggacgc
     3481 catcgccagg tggggccggt cggtctgcag gcgttggatg agcgcgtcgt aggtcgcgag
     3541 gtcggtcgtg ttgtcgaggt cggtcgacgc gccgaacgcg gccccgtgtg ccgcgtcgtt
     3601 gctgtacgcg cacgcggcga gcttgggttt tccgcccacg agaatcgcgt ccgtggccgg
     3661 cgcgcccgtc gcctttcgat agtactcgaa cagggtgggc tcttgcggcc gttcgagtcc
     3721 ctcattatcg aggtactgcc aaacaccggt gagcatggtt gcatggccgg gaatggtgag
     3781 cgtccaaccg aggttgcgat aattggagca cagcgtcccc aacggacgca gctggctcca
     3841 gatttcgtcg acgtgggcgt gcagcgaatc cccgaatgag tcctgataac gcgggccgtc
     3901 gatcacgaac acgatcacct tgggatcgac gggatccgcg gtgaccagct gcttcgactg
     3961 ttcgacggga ggatagacgt cgggctggca ggcgagcgcc gccgccagac acgtagccga
     4021 cacccacggg ccgatcgcga ataaacgctt cgtcgtcatg cccttgcgcc gcgcgtcagc
     4081 gcagcaggac cagcttgcgc gcgaccgatg catcgggtgt gcgcatgcgc acgaagtaca
     4141 cacccgacgc aactcgcttc ccgcccgtgt cgcgaccgtc ccagcccagc cggtgatcgc
     4201 cac
//
LOCUS       DASPGK010000071         4575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_603627, whole genome shotgun sequence.
ACCESSION   DASPGK010000071 DASPGK010000000
VERSION     DASPGK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603627"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VEC56_01905"
     CDS             complement(<1..946)
                     /locus_tag="VEC56_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXV12937.1"
                     /translation="MTPASWQDDILAAGELYRVGGAVRDRLFAIDVADTDFLVRAVAP
                     QDLEKILSRHGVWSRVGKAFGVYKFTPRGGATVDIVFPRTETSIGVGHRDFEVRFDET
                     LPVEADLARRDFTINAIAERIPDGRLVDPFHGRDDLEAGVLRMIFPRAFDEDPLRILR
                     GARFAARFGLAIDGPTRAAMTAAAGLLATVSAERVQDELTKTLSQCERPSLAFDALHQ
                     TGSLVTWLPELERCAGVVQNEYHPDDVYWHTLKTCDRAPRERPVVRWSALLHDLGKVD
                     TRQVITEDGESRVVFYGHEAVGAEVAERVLSRLRYSNEL"
     gene            complement(943..1920)
                     /locus_tag="VEC56_01910"
     CDS             complement(943..1920)
                     /locus_tag="VEC56_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXV12938.1"
                     /translation="MKRFLPWIMPLFGLAVFVVVVQQAGPARIAAVLAEADTAQLVWA
                     PVLVGAIAIARGLRWRYVARSVGIPCGLLRATQVWMIAFFASSVTPAKAGDAVRAVYL
                     RNDSGRPLGECFLTVFVDRLWDLGFVLLAGLASALVFSHRYIAIPSASLFIAGVVVIA
                     GAAALMTHRGVMRALLKPAFLLLTPERHRETLSSSFHTFYDALRNYGESKRRALVMAV
                     LTLVGWALIFTLAVYVSRLLSIPVDPGFVVLIMPIVTLVELIPFTISGLGTRDATVVY
                     FFSVVGVGSAEAVGFSIAYVLIGTYLTALIGLALWVRHPIRWRAAPDAS"
     gene            2035..2250
                     /locus_tag="VEC56_01915"
     CDS             2035..2250
                     /locus_tag="VEC56_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HXV12939.1"
                     /translation="MNDKVTIKIPRPLYNKVQQLIDESGFNSPTDFIVYVLRDVLSER
                     GHRETDRLTPDELDAVKQKLRRLGYLD"
     gene            2279..3439
                     /gene="sat"
                     /locus_tag="VEC56_01920"
     CDS             2279..3439
                     /gene="sat"
                     /locus_tag="VEC56_01920"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HXV12940.1"
                     /translation="MITPHGGTLVSRIVPPSERASQRAKAASLPVFDLSAREASDLLL
                     IAIGAMSPLEGFMRRDQYESVLDRMRLPDGLPWSLPVTLSVPRAVLGDRRAPFSAALR
                     SPEGEIEGVIDVEEVYTPDKAREARLSLGTDDAKHPGVQYLQSAGDTYLGGKVWLIER
                     SKSGPFEAHERDPMETRALFAEKKWDRICAFQTRNPIHRAHEYLIKCALETVDGFLIH
                     PAMGETKSDDVPASARMECYLALTENYFPASHVSLSVFPYAMRYAGPREAILHALLRK
                     NYGCSHFIVGRDHAGVGNYYGTYDAQRIFSQFGADEIGITPVFFEHSFYCRRCGGMAS
                     EKTCPHGAAERVALSGTKVRELLAAGTPLPEEFTRREVSAILERHYAGQRTA"
     gene            3436..>4575
                     /locus_tag="VEC56_01925"
     CDS             3436..>4575
                     /locus_tag="VEC56_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV12941.1"
                     /translation="MSRRLVVIGLDCVTPELLFGPWLSELPNVRRLMADGLHGRLQST
                     IPPITVPAWMSMMTSHDPGTLGVYGFRNRKSYAYDDTFTVNATHVTSKTVWNILSVNR
                     RRSLVLGVPLTYPPKPLNGAMVGCFLTPDRNATFTYPPELQARIDTLAGGEYLIDVKD
                     FRNERRRETLAQIEAMTERRFRAFRALLAEDDFDFAMMVEMGPDRIHHAFWRFIDPGH
                     RLYQRGNEFENVVRDYYIAMDAEIGRTVDALPPDTSVIVVSDHGAKRMVGAVAINEFL
                     IREGYLVLKSYPTSPTRLTADMVDWSATTAWGDGGYYARVFLNIEGREPSGRIAAGDV
                     GRVRREIKDKLEALGDADGNPIGTRVFFPEDVYRACRGIPPDMIVY"
BASE COUNT          831 a         1552 c         1484 g          708 t
ORIGIN      
        1 ccaattcgtt cgagtaccgc aagcgcgaga gcacccgttc ggccacctcg gcgcccaccg
       61 cctcgtgccc gtagaacacc acgcgcgact caccgtcctc ggtaatgacc tggcgcgtgt
      121 ctaccttgcc caggtcgtgc aggagcgccg accagcgcac caccggtcgc tcgcgcggcg
      181 cccggtcgca ggtcttgagc gtgtgccagt acacgtcgtc ggggtggtac tcgttttgga
      241 ccacgccggc gcaccgctcg agctcgggaa gccacgtcac gagcgaaccc gtctggtgca
      301 gcgcgtcgaa cgccaggctg ggtcgctcgc actgcgagag cgtcttggtc aattcgtcct
      361 gaacccgctc ggcgctgacc gtggcgagga gcccggctgc cgccgtcatg gcggcgcgcg
      421 tcgggccgtc gatggccaat ccgaagcgcg cggcgaagcg cgctccccgc aggattcgca
      481 gcgggtcctc gtcgaatgcg cgcgggaaga tcatgcgcag cacgccggct tcgaggtcgt
      541 cgcggccgtg gaacggatcc acgaggcgtc cgtcggggat tcgctccgcg atcgcgttga
      601 tggtgaagtc ccggcgcgcg aggtcggctt cgaccggcag ggtttcgtcg aagcgaacct
      661 cgaagtcgcg atggcccacg ccgatcgacg tctcggtgcg cggaaacacg atgtcgaccg
      721 tggcaccgcc gcggggcgtg aacttgtaga cgccgaaggc cttgccgacc cgcgaccaga
      781 cgccgtgtcg cgagaggatc ttctcgagat cctgcggggc gaccgcgcgc acgagaaaat
      841 cggtgtcggc gacgtcgatc gcgaacaggc ggtcgcgcac cgcgcccccg acgcggtaga
      901 gctcgcccgc ggccaggata tcgtcctgcc acgacgcggg tgtcacgacg cgtccggggc
      961 cgcgcgccaa cggatcggat gccggaccca gagcgccagc ccgatgaggg cggtgaggta
     1021 ggtgccgatc aacacgtagg caatcgagaa gccgaccgcc tccgcgctcc cgacgccgac
     1081 cacggagaag aagtagacga cggtggcgtc gcgcgtcccc aatccggaga tcgtgaacgg
     1141 gatcagctcg accagcgtca cgatgggcat gatcagcacc acgaagccgg gatcgaccgg
     1201 aatcgagagc aggcgcgaca cgtagaccgc gagcgtgaag atgagggccc agccgacgag
     1261 cgtcagcacc gccatcacca gcgcgcgacg cttgctctca ccgtaattgc gaagcgcgtc
     1321 gtagaaggta tggaagctgg agctcaaggt ctcgcggtgc cgctccggtg ttagcagaag
     1381 gaacgcaggc ttgagcagcg cgcgcatgac gccgcggtgc gtcatgaggg cggcggcccc
     1441 cgcgatcacg accacgcctg cgatgaacag cgacgcggac gggatggcga tgtagcgatg
     1501 ggagaacacg agcgcactcg cgaggccggc caacagcacg aagccgaggt cccacaatcg
     1561 gtcgacgaag acggtgagga agcactcgcc gagcgggcgg ccggagtcgt tgcgaaggta
     1621 cacggcgcgt accgcgtcgc ccgccttggc cggcgtcacc gacgacgcga agaacgcgat
     1681 catccagacc tgggtcgccc gcagcagccc gcacggaatc ccgaccgaac gggcgacgta
     1741 gcgccagcgc agtccccgcg cgattgcgat cgcgcccacg aggaccggtg cccacacgag
     1801 ttgcgcggtg tcggcctcgg ccagaacggc cgcgatgcgg gccgggcccg cctgctgaac
     1861 cacgaccacg aagaccgcca gcccgaacag gggcatgatc caaggcagga agcgcttcac
     1921 ggaagatcgg ccatccatat gggttgctgg gggtcccggc aggatcggac ttgatcccgg
     1981 cgccgcgaca cccttataat actccaatct tagtgctcga ggagcctaca atctatgaac
     2041 gacaaggtga ccatcaagat accccgtccg ctctataaca aggtacagca actgatcgac
     2101 gagtcgggct tcaactcccc caccgacttc atcgtctacg tgctgcgcga cgtgctgagc
     2161 gagcgcggac atcgcgagac cgaccgactg acgcccgacg agctggacgc cgtcaagcag
     2221 aagttgcgcc gcctcggtta tctcgactga ccccgtcccc tcgaaggagg accgattcat
     2281 gataacgccg cacggcggga cgctcgtctc gcgcatcgtg cccccatcgg agcgcgcgag
     2341 tcagcgcgcg aaagcggctt cgttgcccgt gtttgatttg agcgcgcgcg aggcttcgga
     2401 cctgttgctg atcgccatcg gcgccatgag tccgctcgaa gggttcatgc gccgcgacca
     2461 gtacgaatcg gtgctcgacc gcatgcgatt gcccgacggg ctgccatggt ccttgccggt
     2521 gacgctgagt gtgccgcgcg cggtgctcgg cgatcgacgg gctcccttct cggccgcact
     2581 ccgctcgccc gaaggcgaga tcgagggcgt gatcgacgtc gaagaggtct acacgccgga
     2641 caaggcgcgc gaagcgcgtc tctccctggg caccgacgac gccaaacacc ccggcgtgca
     2701 gtacctgcag tcggccggag acacctacct cggcggcaag gtatggctga tcgagcgctc
     2761 gaagtcgggg cctttcgaag cgcacgagcg cgacccgatg gagacgcgcg ccctcttcgc
     2821 ggagaaaaag tgggatcgga tatgcgcgtt ccagacccgc aatccgatcc atcgcgcgca
     2881 cgaatatctc atcaagtgcg cgctcgaaac cgtcgacggg ttcctcatcc accccgcgat
     2941 gggcgaaacc aaatccgacg acgtaccggc ttccgcgcgc atggagtgct acctggcgct
     3001 cacggaaaac tacttcccgg ccagccatgt ttcgttatcg gtctttccgt acgcgatgcg
     3061 ctacgcgggg ccacgcgagg cgatcttgca cgcactcctg cgcaagaact acgggtgcag
     3121 ccacttcatc gtgggacgcg accacgcggg cgtcggtaac tattacggca cctacgacgc
     3181 gcaacgcatt ttctcgcagt tcggcgcgga cgagatcggc atcacgccgg tgtttttcga
     3241 acactcgttc tactgccggc gctgcggcgg catggcgtcc gagaagacgt gcccgcacgg
     3301 cgccgctgag cgcgtggccc tgagcggcac caaggtacgc gagctcctgg ccgccggcac
     3361 gccgctcccg gaagaattca cgcgccgcga agtttcggcc atcctcgagc gccattacgc
     3421 gggacaacgc acggcatgag ccgtcgcctg gtcgtcatcg gcctggattg tgtcaccccc
     3481 gagctcctgt tcgggccctg gctctccgag ctgcccaacg tgcgtcgcct gatggcggat
     3541 gggctgcacg gccggctcca gtcgaccatt ccgcccatca cggtgccggc gtggatgtcg
     3601 atgatgacct cgcacgaccc gggaaccctg ggcgtctatg gttttcgaaa tcgtaaatcg
     3661 tacgcctacg acgatacctt caccgtcaat gcgacacacg ttacttccaa gacggtttgg
     3721 aacatactgt ccgtcaaccg gaggcgctcg ctggtgctcg gcgtgccgct gacctacccc
     3781 cccaagccgt tgaacggcgc catggtgggg tgcttcctga ccccggatcg caacgcgacc
     3841 ttcacctacc cgccggagct gcaggcgcgc atcgacaccc tcgcgggggg cgagtatctg
     3901 atcgacgtga aggacttccg caacgagcgc cgacgcgaga cgctggccca gatcgaggcc
     3961 atgaccgagc gccgctttcg tgcctttcgg gcgctcctgg cggaggacga tttcgacttc
     4021 gccatgatgg tggagatggg accggaccgc atccaccacg ccttctggcg cttcatcgac
     4081 ccgggccatc gcctgtacca gcgcggcaac gagttcgaaa acgtcgtgcg ggactattac
     4141 atcgcgatgg acgccgagat cggccgcacc gtcgacgcgc taccgcccga cacgtcggtg
     4201 atcgtggtct ccgaccacgg tgccaagcgc atggtgggtg ccgtcgccat caacgagttc
     4261 ctgattcgcg agggttacct ggtgctcaaa tcgtatccga cttcccccac gcgactgacc
     4321 gcggatatgg tcgactggag cgcgaccacg gcctggggcg acggcggcta ctacgcccgc
     4381 gtgtttctca atatcgaagg ccgcgagccc agcggccgca tcgccgccgg cgacgtcggc
     4441 cgcgttcgcc gcgagatcaa ggacaaactg gaggcgctgg gtgacgccga cgggaacccc
     4501 atcggtaccc gcgtgttctt ccccgaagac gtctatcgcg cgtgtcgcgg catccccccc
     4561 gacatgatcg tctat
//
LOCUS       DASPGK010000072         5989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_369138, whole genome shotgun sequence.
ACCESSION   DASPGK010000072 DASPGK010000000
VERSION     DASPGK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369138"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VEC56_01930"
     CDS             complement(<1..270)
                     /locus_tag="VEC56_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXV12942.1"
                     /translation="MAKYPHAEIEAKWQERWARDGLMRCDLSKTAQKYYCLMMFPYPS
                     GELHVGHGRNYVIGDSLARFKKMEGFEVLAPMGWDAFGLPAENAAI"
     gene            complement(301..528)
                     /locus_tag="VEC56_01935"
     CDS             complement(301..528)
                     /locus_tag="VEC56_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXV12943.1"
                     /translation="MDQEVRVNARGLSAPGPRMMVDAALEKEQPALMRVVVSNAPAAE
                     DVRRYLEARGAQVEIDGVGTEFHVLARFPRP"
     gene            complement(611..1678)
                     /gene="holA"
                     /locus_tag="VEC56_01940"
     CDS             complement(611..1678)
                     /gene="holA"
                     /locus_tag="VEC56_01940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXV12944.1"
                     /translation="MAGRLSGYKKLFDEVRQGRVKALYFLHGPEEYMKREFVRELVGA
                     ALPDAERTFNLDVLHGDEFDAQAFDDRLMSFPLFSARRVVILRNFEALSPSNRERVIE
                     RAGGVPDSMTLVVESNDEKLETAAHKKLAALAAQRGVAFAFGPLDESESLERALARFR
                     REGVQIDPDALDLLVESVGTQLMDLANEIDKILLSASDTKTVTRERVASVVGKYRTES
                     LFSLLDGVGVAAPATTLSRLSSLLDAGEEPVFVVAMMLRRVVALLEVRHVVAERGRAV
                     SSDRALADSIAATQSPYFAGKLREQAARTTSRALETLLANLRWADLRLKTTNLDPKCL
                     VEEALVAAHVGKALASPALAP"
     gene            complement(1680..2201)
                     /locus_tag="VEC56_01945"
     CDS             complement(1680..2201)
                     /locus_tag="VEC56_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HXV12945.1"
                     /translation="MKPKKSRRRRSGLVWVALAASLGVACGYSTTSRTAKDIKSIYVP
                     FFENETAEPNLEISVTERVIRNLVADNTLKVVSEDAADAVLDARIVEFKNQPFSFNQD
                     LNAEEYIVVIKVVATLFNRRTNEAIWQDRSFQGDGSYFIESVGEGRTFTDAIDESLRE
                     ITERILNLTVQDW"
     gene            complement(2170..3591)
                     /locus_tag="VEC56_01950"
     CDS             complement(2170..3591)
                     /locus_tag="VEC56_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV12946.1"
                     /translation="MKSVILLVDDQDTIRFFLEKTLKQEGYEAVTARTGAEAIEKARE
                     VVPDLVLLDLKLPDMDGLEVLRRIKDVFPEIGVVMITAFGDIETAVTAMKNGAYDFVS
                     KPINLEQLLMVIRKGLASERLNREVLELKRQMDPEVGFDYILGETGAMKRVYEIVQQV
                     AKSDTTTVLIEGESGVGKEMVARLIHRYSNRANKPFMDINCASLPETLLESELFGYEK
                     GAFTDAKGQKQGLLELANRGSLFLDEIGEMSLTIQVKLLRVLERMEFRRLGGTQDIHV
                     SVRVISATNRDLQKEVDEGRFRADLFYRLKVVPLYIPPLRERKEDLLKFVNYFINMFN
                     TKFAKNFNQISDDARHALLSYPWPGNIRELKNVIERVVLLETGPVLKREMLPFATQRV
                     DEGSIGRRIDAILSSPLPEDGVDFEGLVGDFERALILKASEESGWNQSKTARLLNVKR
                     DKLRYRMKSFNLGDETEEVPTTS"
     gene            complement(3563..5641)
                     /locus_tag="VEC56_01955"
     CDS             complement(3563..5641)
                     /locus_tag="VEC56_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV12947.1"
                     /translation="MRIFAYIPRRRLLLTVAAVATILALLATALPWWATIPLAVLLCA
                     LYVARESIAVRNRYEGVLEGIRKIHGDAIGELSSVRPGEIDAAIYKALKEIATELERK
                     NFQLVEKNIQLLSIKEIGLTLVSSLDESKVVDAVINFLSKGLGYRELFVGIFHPDEEA
                     FNLYVFRDTPEGHVHAQVRVSLEELDGLLRKSVQMHQSILIRDPEMHPVGSAGGAPLF
                     RDSTMSSYIVVPLVKSGATQGCESRNDCVLHMTQVRRETASIEHGFRCPACDRIPLLG
                     VVGVTDGFKAASLSRVDLVSVETLAVQLSTMLENNSLFAELQQEESFRDNVINSMMNG
                     LITVDQRGRVLFANALAEEITGHTVAELRQLRVQDLINDSPTGTGENPVMRTLRTGRR
                     AFQQEAWLAKKDGTKDPVVLNTTLLFDEKKQPQGALAVFNDITRQKRMEEQISHLDKL
                     AALGRLSSSIAHEIRNPLTGIAAGIQYLQRAGQVADSQRENIEFILDEVKRIDRLIGD
                     LMSVVRVSDLIYEETSLEELIRNGIASMSDLAQRKSVTVTTEFPGESRPIVVDADRIT
                     QVMINLLKNAIEASPADASVTVSLSFSPDVRDVLFDAVGDFAIIRVRDNGLGLTEEDK
                     QRVFEPFFSKKSGGTGLGLYVTHSIIERHSGYIYVDSEYGVGTTFSVYLPVKQVQHGD
                     SREIGHPVGG"
     gene            complement(5671..>5989)
                     /locus_tag="VEC56_01960"
     CDS             complement(5671..>5989)
                     /locus_tag="VEC56_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12948.1"
                     /translation="AIHSDLGTRITTVSTWQAGYYIPAVEPGAGHIDLVIDSLVLQPN
                     RYYLSMWISTVEEWYDVLDHCIAIDVEAADALDNQGRGLHPKWGLVFTPSRWSLGPPV
                     AAR"
BASE COUNT          981 a         1936 c         1970 g         1102 t
ORIGIN      
        1 gatggcggcg ttctcggccg gcagcccgaa ggcgtcccag cccatcgggg ccagcacctc
       61 gaaaccctcc atcttcttga agcgcgccag cgagtcgccg atcacgtagt tgcgcccgtg
      121 gccgacgtgg agttcgccgg aggggtacgg gaacatcatc aggcagtagt atttctgggc
      181 ggtcttcgac agatcgcagc gcatgagccc gtcgcgggcc cagcgttcct gccacttggc
      241 ttcgatctct gcgtggggat acttcgccat aaacgaacgt tgaaggcgcg gccgccgcgg
      301 ctacgggcgc ggaaagcgcg cgaggacgtg gaactcggtg ccgaccccgt cgatctcgac
      361 ctgggcgccg cgggcttcca gatagcgccg cacgtcctcg gccgcgggcg cgttactgac
      421 cacgacgcgc atcagcgcgg gctgctcctt ctccagcgcg gcgtcgacca tcatgcgcgg
      481 gccgggcgcg gatagcccgc gggcattgac tcgaacttct tgatccatag gagattgcgg
      541 ctactggtta agaccgtttc gaagcgccgg gcccccgctg cgccgccgcg gcgctcacga
      601 ggaggtcact ctacggcgca agcgcggggc tggcaagcgc tttccccacg tgcgcggcga
      661 ccagggcctc ttcgaccagg cacttgggat cgaggttcgt cgtcttgagc ctgaggtcgg
      721 cccagcgcag attcgcgagc agtgtctcca gggcgcgtga ggtggtgcgc gcggcctgct
      781 cgcgcagctt gccggcgaag tacgggctct gggtggcggc gatggagtcg gccagggccc
      841 ggtccgacga caccgcgcgg ccgcgctcgg cgacgacgtg gcgtacctcg aggagtgcca
      901 ccacgcggcg cagcatcatc gcgaccacga agacgggctc ctcgccggcg tcgaggaggg
      961 acgacaggcg cgagagcgtc gtggccgggg cggccacccc cacgccgtcc aggagcgaaa
     1021 agaggctctc ggtccggtac ttccccacca cgctcgccac ccgctcgcgc gtgacggtct
     1081 tggtgtcgga ggcggacagc aggatcttgt cgatctcgtt ggccaggtcc atgagctgcg
     1141 tccccacgct ctccaccagg aggtcgaggg cgtcggggtc gatctggacg ccctcgcgcc
     1201 ggaagcgcgc cagcgcgcgc tccagactct ccgattcgtc gaggggcccg aacgcgaacg
     1261 ccacgccgcg ctgcgccgcc agcgccgcca gcttcttatg ggccgcggtc tcgagcttct
     1321 cgtcgttgga ctcgaccacc agcgtcatcg aatccgggac cccgcccgcg cgctcgatca
     1381 cgcgttcgcg gttggacggc gaaagcgcct cgaagttgcg caagatgacg acgcgacgcg
     1441 ccgagaagag cgggaacgac atcaggcggt cgtcgaaggc ctgcgcgtcg aactcgtccc
     1501 cgtggagcac gtcgaggttg aaggtgcgtt ccgcgtcggg tagcgcggcg ccgaccagct
     1561 cgcgcacgaa ctcgcgcttc atgtactcct cgggcccgtg caggaaatag agcgccttca
     1621 cgcgcccctg gcggacctcg tcgaagagct tcttgtatcc cgaaagacgt cccgccatgt
     1681 caccagtcct gcacggtgag gttcaggatg cgctccgtga tctcgcgcag cgactcgtcg
     1741 atcgcgtcgg tgaaggtgcg cccctccccc accgactcga tgaagtagga gccgtcgcct
     1801 tggaacgagc ggtcctgcca aatggcctcg ttggtccggc gattgaacag ggtcgccacc
     1861 accttgatca cgacgatgta ctcttccgcg ttgaggtcct ggttgaagga gaagggctga
     1921 ttcttgaact cgacgatgcg cgcgtcgagg acggcatcgg ccgcgtcttc ggacaccacc
     1981 ttgagggtgt tgtcggcgac caggtttcgg atcacgcgct cggtcaccga aatctcgagg
     2041 ttgggctcgg ccgtctcgtt ctcgaagaac ggcacgtaaa tcgacttgat gtccttggcg
     2101 gttcgcgagg tggtgctgta gccgcacgcc acccccagcg aggcggccag cgccacccag
     2161 accaatccgc tacgacgtcg tcgggacttc ttcggtttca tcgccgaggt tgaagctctt
     2221 catgcggtag cggagcttgt cgcgcttgac gttcaagagg cgcgcggtct tgctctggtt
     2281 ccagcccgac tcctcggagg ccttgagaat cagcgcgcgc tcgaaatcgc ccaccagccc
     2341 ttcgaagtcg acgccgtcct cgggcagcgg cgacgagaga atggcgtcga tgcggcgacc
     2401 gatgctcccc tcgtccacgc gctgggtcgc aaacggcagc atctcgcgct tcaagaccgg
     2461 tccggtctcc aagaggacca cgcgctcgat cacgttcttg agctcgcgga tgttccccgg
     2521 ccacgggtac gagagcagcg cgtggcgcgc gtcgtcggag atctggttga agttcttcgc
     2581 gaacttggtg ttgaacatat tgatgaagta gttcacgaac ttgaggaggt cctctttgcg
     2641 ctcgcgcagc ggcggaatgt acagcgggac caccttcaag cgatagaaga ggtcggcgcg
     2701 gaagcgccct tcatccactt ccttttgcag gtcgcggttg gtggcactaa tgacgcgtac
     2761 gctgacatgg atgtcctggg ttccgcccag gcgccggaac tccatgcgct cgagcacgcg
     2821 caggagtttg acctggatgg tgaggctcat ctcgccgatt tcatccagga acaggctccc
     2881 gcggttggcc aactcgagca gcccttgctt ctgccccttg gcgtcggtga aggccccctt
     2941 ctcgtagccg aacaattcgc tttcgagcag cgtctcgggc aggctcgcgc agttgatgtc
     3001 catgaagggc ttgttggcgc gattcgaata gcggtggatc agcctcgcga ccatctcctt
     3061 gcccaccccg ctctcgcctt cgatcaggac ggtggtggtg tcgctcttgg ccacctgctg
     3121 gacgatctcg tagacgcgct tcatggcacc cgtctcgccc aggatgtagt cgaagccgac
     3181 ctcggggtcc atctggcgct tgagctccag gacctcgcgg ttcaggcgct cgctggcgag
     3241 ccccttgcgg atcaccatca ggagctgctc gaggttgatg ggcttggaga cgaagtcgta
     3301 ggcgccgttc ttcatggccg tgacggcggt ctcgatgtcg ccgaaggccg tgatcatgac
     3361 caccccgatc tccgggaata cgtccttgat gcgccgcagg acctcgagcc cgtccatgtc
     3421 cgggagcttg agatcgagca ggaccaggtc gggcacgact tcgcgcgcct tctcgatggc
     3481 ctcggcaccg gtacgcgccg tcaccgcttc gtacccttcc tgcttcaggg tcttttcgag
     3541 aaagaagcga atcgtgtcct ggtcatccac caacaggatg accgatttca cgggagtctc
     3601 catgctgcac ctgcttcacg ggtaaataga ccgagaacgt cgtccccacg ccgtactcgc
     3661 tgtcgacgta gatgtagccg ctgtgacgct cgatgatgct gtgcgtcacg tacaggccca
     3721 gtccggtacc gcccgacttc ttggagaaga atggctcgaa gacgcgttgc ttgtcttctt
     3781 ccgtgagccc caacccgttg tcgcgcacgc ggatgatagc aaaatcaccc accgcgtcaa
     3841 acagaacgtc gcgtacgtcg ggcgaaaaag acagcgatac ggtgacgctg gcgtcggccg
     3901 ggctggcttc gatggcgttc ttgagcaggt tgatcatgac ctgggtgatg cgatcggcgt
     3961 cgaccacgat gggccgcgac tcgcccggga actcggtggt cacggtcacc gacttgcgct
     4021 gggccaggtc ggacatgctg gcaatgccgt tgcggatcag ctcctcgagc gacgtctcct
     4081 cgtagatgag gtcgctcacg cgcaccaccg acatcaggtc gccgatcagc cggtcgatgc
     4141 gcttgacctc gtccaggatg aactcgatgt tctcgcgctg gctgtccgcc acctggccgg
     4201 cgcgttggag gtactggatg ccggccgcga tgccggtcag cgggttgcgg atctcgtgcg
     4261 cgatgctgga ggagagccgg cccagcgccg ccagcttatc gaggtgcgag atctgctcct
     4321 ccatgcgctt ctggcgcgtg atgtcgttga ataccgccaa ggcgccctgc ggctgcttct
     4381 tttcgtcgaa gagaagggtc gtgttgagca ccaccgggtc cttggtgccg tctttcttgg
     4441 ccagccacgc ctcctgctgg aaagcgcgcc ggccggtgcg gagcgtgcgc atcaccgggt
     4501 tctcccccgt cccggtgggg ctgtcgttga tgaggtcctg cacgcgcagt tggcgcagct
     4561 ccgccaccgt atggcccgtg atctcttcgg ccagcgcgtt ggcgaacagc acgcgaccgc
     4621 gctgatcgac ggtgatgagc ccgttcatca tgctgttgat gacgttgtcg cggaagctct
     4681 cttcctgctg gagttcggcg aacaggctgt tgttctcgag catggtggaa agctggaccg
     4741 cgagggtttc caccgagacc aagtcgacgc gcgagagcga ggccgccttg aagccgtcgg
     4801 taacgccgac cacgccgagc agcggtatgc gatcgcaggc cgggcagcgg aacccgtgct
     4861 cgatggacgc cgtctcgcgg cggacttgcg tcatgtgcag cacgcagtcg ttgcgcgatt
     4921 cgcacccctg ggtggcgccg ctcttgacca aggggacgac gatgtacgag ctcatggtcg
     4981 aatcgcgaaa caagggcgcc ccgcccgcgc tccccaccgg gtgcatttcg gggtcgcgaa
     5041 tcaaaatcga ctggtgcatc tgcaccgact tgcgcaacag gccgtccagc tcctcgagcg
     5101 acacccgcac ttgcgcgtgc acgtggccct cgggcgtgtc gcggaagacg tagaggttga
     5161 acgcttcctc gtccgggtgg aagatgccga cgaacaactc gcggtaaccc aagcccttcg
     5221 acaggaagtt gatgaccgcg tcgaccacct tcgactcgtc caacgaggag accagcgtga
     5281 gcccgatctc cttgatgctc aagagctgga tgttcttctc gaccaactgg aagttcttgc
     5341 gctcgagctc ggtcgcgatt tccttgagcg ccttgtagat ggctgcgtcg atctcgcccg
     5401 ggcgcacgct cgagagctcg ccgatggcgt cgccgtggat cttgcggatg ccctcgagca
     5461 cgccttcgta gcggtttcgc accgcgatcg actcgcgcgc gacgtagagc gcgcacaaga
     5521 ggaccgcgag cgggatcgtc gcccaccacg ggagtgccgt ggccagcagg gcgaggatcg
     5581 tcgcgaccgc ggcgacggtg agtaagaggc ggcggcgagg tatgtacgcg aagattctca
     5641 tggtcccttg cgcgggccgc cggccgacgt ctagcgcgcg gccaccggcg gtcccaggct
     5701 ccagcgcgac ggcgtgaaca ccagccccca cttcgggtgc aggccgcggc cctggttgtc
     5761 gagtgcgtcg gcggcctcga cgtcgatcgc gatgcagtgg tcgaggacgt cgtaccactc
     5821 ctcgacggtc gagatccaca tggacagata atagcggttg ggctggagga ccaggctgtc
     5881 gatgacgagg tcgatgtgcc cggcccccgg ctccaccgcc gggatgtagt agcccgcctg
     5941 ccaggtgctc acggtcgtga tgcgcgtccc caagtcggaa tggatggcc
//
LOCUS       DASPGK010000073         9350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_18055, whole genome shotgun sequence.
ACCESSION   DASPGK010000073 DASPGK010000000
VERSION     DASPGK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18055"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..773)
                     /locus_tag="VEC56_01965"
     CDS             complement(60..773)
                     /locus_tag="VEC56_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXV12949.1"
                     /translation="MKALVYQGRGRRSFEDKPRPAIKAPTDAIVKVTNPTVCGSDLHA
                     MRGDAPRIARGRTLGSEGTGIVERVGDRVSNFRAGDSVLISGLTSCGTCVHCERGQPA
                     RCENGGWLLGCEIDGTCAEYVRVPFADYSLLSVAGAGSRDTDGPWIDNLPEGFVRNVI
                     HGPDERIDTAPIVFGGSVGMGPLLTVMQYYRTVLRPSARSVRTSVSGHPESRKPALRN
                     NRRGLRQHLLHYFAPTRTR"
     gene            complement(868..1014)
                     /locus_tag="VEC56_01970"
     CDS             complement(868..1014)
                     /locus_tag="VEC56_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXV12950.1"
                     /translation="MLWTIAVIMLVLWLLGLITSYTMGGFVHVLLVIAIVIVLLRVIR
                     GRRV"
     gene            complement(1043..2155)
                     /locus_tag="VEC56_01975"
     CDS             complement(1043..2155)
                     /locus_tag="VEC56_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12951.1"
                     /translation="MTRFLQRLIAGARQAHSTELGSKGCVALAVLLASCGTVPPARQE
                     GQIAASPPRYSLVFIIHGDGDYLYHDALGNARRADEDVLSRAQAIAERNPNAEVFIFH
                     QIERRHVLFLIPRRDGRAYYYRHGHLREQKAYWRDQGDSRFDPEVRLYEQFAGTQPLP
                     PVRLFFYFGHELPELNGAGYDASYSERRVSIHDLAEGVRAIAGESAKIDLLALATCFG
                     GTPYTVGALAPHARYIVASPDNLHLSYFDLEPLAGLDFGSSDGDITAFANRFARNAFE
                     QLARDVETVVSVVVYDVDAVSGFVDSVADAYARTVTAADGTIPAPIERCDCADEVTYT
                     SPGMNDGLTVFYRAPRFGRMKNEPRHSGWECWRATK"
     gene            complement(2175..2609)
                     /locus_tag="VEC56_01980"
     CDS             complement(2175..2609)
                     /locus_tag="VEC56_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12952.1"
                     /translation="MFTKTVIRMLFVIGALGYAGVAMGQDDVHNYLNDTAIKVKATES
                     PAEKREILSTSLGGMIKALDTVKSAPMTSDRDDAKLDRIQATLQEKSDELAGVNGFDR
                     VPDDRLNAFSTYIVQDMEQADKTFTISLVTALLIVIIIILLV"
     gene            2775..3944
                     /locus_tag="VEC56_01985"
     CDS             2775..3944
                     /locus_tag="VEC56_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXV12953.1"
                     /translation="MSTLPSTADVAIVGAGPTGLALASVLAAKDVSFVLVDRLAEGAN
                     TSRAAVIHARTLEVLDELGVTERLRAEGHVVPRFTLRDRDRALATIDFGRLPTRYPFT
                     LMIPQFRTEAILADRLRELGGRLYRGFDVVDVRQDADGASLTVAAEGRPSHELRARYV
                     VGADGMHSTVRECAGIGFTGDKYDQSFVLADVRMSWPLRLDEVMLFLSPDGLVVVAPL
                     PGGRHRVVATMDDAPEHPNLADVQRLLDTRGPTTGTTRVDEIVWGSRFRVHHRVADRY
                     RAGRILLAGDAAHVHSPAGGQGMNTGIQDAVALAHALAEVLRERASESRLDEYERTRR
                     PVAKHVVALTDRATRMATLRSRRARALRNAAIGVMGRIPAVRRRIATELAELRYR"
     gene            complement(3952..4467)
                     /locus_tag="VEC56_01990"
     CDS             complement(3952..4467)
                     /locus_tag="VEC56_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV12954.1"
                     /translation="MTKLNVDDIVRLLDLRPLPEEGGLFRETYRSANALDGSALPASY
                     RGPRSLATAIYYLITPELFSAMHRVRGDEVFHFYFGDPVEMLQLRADGTGERLTLGSD
                     VALGMRPQVVVAGGVWQGSRLRPGGSFALLGATMAPGFDVADFEIGQRMPLVRRYPDF
                     AEEIARLTRGE"
     gene            complement(4492..5451)
                     /locus_tag="VEC56_01995"
     CDS             complement(4492..5451)
                     /locus_tag="VEC56_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12955.1"
                     /translation="MPAFIRSSIQPVALLLVSTVVVASCSSGDKVDNDFDTHVAVPAY
                     SGPGPRVLFDEGHHNVHRAYKSYRPFVELIESDGYRVAQSTNEIRAEALDGFDVFVIA
                     AALGDNDVNDEPAFEETECDAVRDWVAGGGALLLIVDHFPVGDANQRLASRFGVQLET
                     GAAEDSVHYDPAFDRTHIVFSRDNGGLGMHPIVEGHDAATMIDRVLTFTGTAVYAEPP
                     AVGFLVLTSSAFARPPQPVVVRDGGDVVVNVAYGDPVAVPGWSQGLAMEFGKGRVVVL
                     GEAAMLTARLHRFDGRPIGMNTPGYDNRQLALNIMHWLTRAPE"
     gene            complement(5598..6047)
                     /locus_tag="VEC56_02000"
     CDS             complement(5598..6047)
                     /locus_tag="VEC56_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12956.1"
                     /translation="MKGRGLALPAGALLAGVLLSGGAMCIGGACSRDDAARLKRQERR
                     QFSIVMESKMRQLDRGIASLGDTPTLTDSVFASEVEDLKRNQQALRSRLAAMSAVPER
                     EWPALRDSVETDYHHVREQYGALVHHETLAEPAPADSSMGDAPSLPR"
     gene            complement(6071..6811)
                     /locus_tag="VEC56_02005"
     CDS             complement(6071..6811)
                     /locus_tag="VEC56_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV12957.1"
                     /translation="MMIATPTSQTRRVAVPEIAQLVECASLRTMELFRDVTSSDLARV
                     AGITRFQSVDKRTRIGLQSDPGNLSFLMDGLAKVSRLDGNGGETVLYMMKPGEAYGVP
                     VSDYREDVSITTLQSSVVARVRVRELEQIVGSARVQAEISRIRSLRMAQMEDRLDEMS
                     GRVPARTARILLRLCKEFPKALHCGTKVNVLLTQQDIASMIGATREVTSSTLNVFRRR
                     GWLGIHDRYMCIHDQDGLYEATSCASAV"
     gene            6975..7691
                     /locus_tag="VEC56_02010"
     CDS             6975..7691
                     /locus_tag="VEC56_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV12958.1"
                     /translation="MRVLVVEDNPKMAEAIRKGLVQHNYAVDVFGRGYEAEEAAAGAD
                     YDAIVLDLMLPDRDGVDVCRNLRRRGIATPILMLTALADTESKVAGLDAGADDYLTKP
                     FEFEELVARIRTLMRRGRAVESRVLKYGDLEFDLNARRITRAGRAIQLSGKETALLEY
                     FMRNPDRVVARQTLVEKIWDTSYQPVSNAIDVHISSLRKKIDRDFDESFIHTVVGAGY
                     RFGRTHGDVPGKTATDADTK"
     gene            7688..9130
                     /locus_tag="VEC56_02015"
     CDS             7688..9130
                     /locus_tag="VEC56_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV12959.1"
                     /translation="MTSLPLRLKLTLWFVTLFASIYMGLIGVVWFIGRSSQNRLLDEA
                     LITVARGVGGLLVDIEGDFSNLDLGPYQPVDREFSLLAVRDGNGVVVASDLRVDTGAL
                     PPLPPPDQESTITRINGDLARSLVGRAIQTRLVTYRVSSRDGVVHYIDLARATEFGRR
                     QRQLFSDVIFIAGLGGVFSSALAAWFLVGRATTPFERLAEIARRVGPESETRFPSGSS
                     GREIERLQTELNEALGRLEEGYRVRQRFLANVAHDLKTPISVMLTQSQVIEPEAASLD
                     EFRLYRNSMIEELRRLRGIIEGILALSRASQGEALLRRSQVPVNTLLAQSALRCKAQA
                     DAAQVRLKVVELDGDENRELFGDFDLLCTMLDNLIRNAIRFSPPRETVHLEAELADGR
                     ARLSVRDHGPGIPEEYLDRIFERFIQVPDEDGKVRGGTGLGLAIAKTVAEAHGGTIEA
                     RNRRRGGCAFTISLPLSATAPAANAASTRV"
BASE COUNT         1622 a         3036 c         3061 g         1631 t
ORIGIN      
        1 ccaccatcgc cacgagtagt gaagtcgctc gtttcatggg ttgctccttc tcgttgtgcc
       61 tacctcgttc tcgtaggcgc gaagtaatgc aagagatgct ggcgtaaacc ccttcgattg
      121 ttgcgaagcg cgggtttgcg cgactcggga tgaccactga cgctcgtgcg cacgctgcgt
      181 gcgctgggac gaagcaccgt ccggtagtac tgcatgacgg taaggagcgg gcccattccg
      241 accgaacccc cgaagacaat gggggccgtg tcgatgcgtt cgtccggccc atgaatgaca
      301 ttgcgcacga agccctcggg aagattgtcg atccagggcc cgtccgtatc gcggctaccc
      361 gcgccggcaa ccgagagcag gctatagtcc gcgaacggca cgcggacgta ctccgcgcaa
      421 gtcccgtcga tctcgcatcc gagtaaccaa ccgccgttct cgcagcgcgc cggttgtccc
      481 cgctcgcagt gcacgcaggt cccgcacgac gtcaggcccg agatcaacac gctatcgccc
      541 gcgcggaagt tggagacgcg gtcgccgact cgttcaacga tgccggtgcc ttcgcttccg
      601 agagttcgcc cgcgggcaat gcgcggggca tcacccctca tggcgtgcag gtccgagccg
      661 caaaccgtcg ggttcgtcac tttgacaatg gcgtccgtag gggccttgat ggcgggcctg
      721 ggcttgtcct cgaaggaacg cctgccccgg ccttgataaa cgagcgcttt catctcggat
      781 ctccctttgc tcatccaatt agggcaagac cgtgccccgc tgaacgggac gacgacccag
      841 ccattgacta ccgaccggga gcgacggtta cacccgtcgg ccccggatga cacgcaacag
      901 tacgatcacg atggcaatga ccagcaggac gtgaacgaac ccgcccatgg tgtacgacgt
      961 gatcagtcca agcagccaaa gcaccaacat gatgacagcg atggtccaca gcatgatgcc
     1021 cacctccttc acggttaaag agctacttcg tcgcgcgcca gcattcccaa ccggagtggc
     1081 gcggttcatt cttcatgcgt ccaaatcggg gcgcgcggta aaagaccgtg agcccatcat
     1141 tcatcccggg cgacgtgtag gtcacctcgt cggcacagtc acaccgttcg atgggtgcgg
     1201 gtatggttcc atctgcagcg gttaccgtgc gggcgtacgc gtcggccacc gaatcgacga
     1261 atccactcac ggcatccacg tcgtagacga cgacactgac cacggtttcc acgtctctgg
     1321 cgagttgctc gaacgcattg cgcgcaaaac ggttggcgaa ggcggtgatg tcgccgtcgc
     1381 tcgatccgaa gtcgagtccg gccagcggct cgaggtcgaa gtaggacaga tggaggttgt
     1441 cgggcgacgc gacgatgtat cgcgcgtggg gcgcgagcgc gcccacggtg tagggtgtgc
     1501 cgccgaaaca cgtagcgagt gccagcaggt cgatcttcgc cgactcaccg gcgatcgctc
     1561 gtacgccttc ggccaggtcg tgaatgctga cacggcgttc cgagtacgag gcatcgtagc
     1621 cggcgccgtt gagctcgggg agttcgtggc cgaagtagaa gaacaaccgc acgggaggga
     1681 gcggctgcgt cccggcaaac tgctcgtaga gacgcacttc gggatcgaac cgcgagtctc
     1741 cctgatcgcg ccagtacgct ttctgctccc ggaggtgccc gtgtcgatag tagtaggcac
     1801 ggccgtcgcg gcgcggaatg aggaacagca cgtgccggcg ctcgatctgg tggaagatga
     1861 acacctcggc gttcggattg cgctccgcga tcgcctgcgc tctggagagg acgtcttcgt
     1921 ccgcacggcg tgcgttgccg agcgcgtcgt ggtagaggta atcgccgtcg ccgtggatga
     1981 tgaacacgag cgaatagcgc ggcgggctcg cagcgatctg cccctcctga cgggcgggcg
     2041 gcaccgtacc gcacgacgca agtaagaccg cgagcgccac gcaccccttg cttcccagtt
     2101 cagtcgagtg tgcttgcctg gcgcccgcga tcagcctttg cagaaaacgt gtcatcgatt
     2161 cgaaggtccc cgcgttagac caatagaatg atgatgatga cgatcaggag cgccgtgacg
     2221 aggctgatcg tgaaggtctt gtcggcctgt tccatatctt gtacgatgta tgtggagaag
     2281 gcgttgagtc gatcgtcggg gacgcggtcg aagccattga cgccggcgag ttcatcgctt
     2341 ttttcttgca gcgtggcctg gatgcgatcc aatttggcgt cgtcccgatc cgacgtcatc
     2401 ggcgcactct tgacggtgtc gagcgccttg atcatgccgc cgaggctcgt gctcaggatc
     2461 tcacgcttct cggcgggact ctcggtagcc ttcactttga tggcggtgtc gttgagatag
     2521 ttgtggacgt cgtcctgacc cattgcaacc ccggcgtagc ccagggctcc gatcacgaaa
     2581 agcatgcgaa tgacggtttt ggtaaacatg acgttctcct ttggtctccg gggcgccatg
     2641 cgaagtcggg cgcgttgaga cgcttgaatt ggtttctgtt gtctcctggc caggatacgg
     2701 gagaccgccc gtgggattga atcgaccgaa ggggtgaaag gggagctagt ccttaggatg
     2761 gtcgtttcgc cgtgatgagt acacttccgt cgaccgccga cgtcgccatc gtcggcgccg
     2821 gccccactgg actcgcgctc gcgagcgttc tcgccgccaa ggacgtttct ttcgtcctcg
     2881 tcgaccgcct cgccgaagga gcgaatacgt cgcgcgccgc ggtgatccat gcccgcacgc
     2941 tcgaagtgct cgacgaactg ggcgtcaccg agcgcctgcg cgccgagggt cacgtcgtgc
     3001 cgagattcac gctgcgagac cgcgatcgcg cgctggcgac gatcgacttc ggccggctgc
     3061 ccacgcgcta cccgttcacg ttgatgattc cacagttccg caccgaagca atcctggcgg
     3121 accggcttcg ggagttgggc gggcgtctgt atcggggatt cgacgtcgtc gacgtgcgtc
     3181 aggacgccga cggtgcttcg ttgaccgtcg cggccgaagg ccggccgtcg cacgaacttc
     3241 gcgcgcggta cgtcgtcggt gccgatggaa tgcatagcac cgtgcgcgaa tgcgcgggga
     3301 tcggattcac cggagacaag tacgaccaat cgttcgtgct cgcggacgtg cgcatgagct
     3361 ggcccctgcg cctcgacgaa gtgatgctgt ttctgtcacc cgacgggctc gtggtggtgg
     3421 caccgctgcc gggcgggcga caccgcgtgg tcgcgaccat ggacgacgcg cccgagcacc
     3481 cgaacctggc ggacgtgcag cggctgctgg atacgagggg accgacgacc ggaacgacgc
     3541 gcgtcgacga gatcgtgtgg gggtcgcggt ttcgcgtgca ccaccgcgtg gcggatcgct
     3601 accgagccgg gcggattctg ctcgcgggtg acgcggcgca cgtgcacagc ccggcgggag
     3661 gacaggggat gaataccggc atccaggacg ctgtcgcgct cgcccacgcg ctggcggaag
     3721 tgctaagaga acgggcgagc gaaagccggc tcgacgagta cgaacgaact cgtcgtcccg
     3781 tcgcgaagca cgtggtggcg ttaaccgacc gcgcgacgcg catggcgacg ctgcgctcgc
     3841 ggcgcgcacg tgcgcttcgc aacgcggcca tcggagtgat gggccgaatc cccgcggtcc
     3901 ggcggcggat cgccaccgag ctggccgagc tccggtatcg atgagcgccg actattcgcc
     3961 ccgggtcagg cgcgcgatct cttccgcaaa atccggataa cgccgcacga gcggcattcg
     4021 ctggccgatc tcgaaatccg cgacgtcgaa tcccggcgcc atggtggctc cgagcagcgc
     4081 gaaagatccg ccgggccgca gcctcgaccc ttgccacacg ccacccgcca cgacgacctg
     4141 cgggcgcatg ccgagcgcca cgtcgctgcc cagcgtgagt ctctcgccgg tgccgtcggc
     4201 gcgcagctgc agcatctcga ccggatcacc gaaatagaaa tggaacactt cgtctccgcg
     4261 cacgcgatgc atcgcggaga agagctcggg agtgatcaga tagtagatag ccgtcgcgag
     4321 cgagcgggga ccgcgatagc tcgcggggag cgcgcttccg tcgagcgcgt tcgcggatcg
     4381 atacgtctcc cggaagagac cgccctcctc ggggagcgga cgcagatcca gcaagcgaac
     4441 gatatcgtcg acgttgagct tggtcattcc cgtctcccgc gcgcgagagt atcactccgg
     4501 ggcgcgcgtc aaccagtgca tgatattgag cgcgagctgc cggttgtcgt agccgggcgt
     4561 gttcatgccg atcggtcgcc cgtcgaagcg gtgcaggcgc gcggtcagca tggcggcctc
     4621 gccgagaact acgacgcgcc cctttccgaa ctccatcgcg agcccctgcg accagccggg
     4681 aaccgcgacc ggatctccgt acgcgacgtt gacgacgacg tcgccgccgt ctcgcacgac
     4741 gaccggttgc ggcggtcgtg cgaaggcgct gctcgtcaac acgagaaagc ccaccgccgg
     4801 cggctccgcg tagaccgcgg tgccggtgaa tgtgagcacg cggtcgatca tggtcgcggc
     4861 atcgtgcccc tcgacgatcg ggtgcatccc cagcccgccg ttgtcccgcg agaacacgat
     4921 gtgcgtgcgg tcgaacgccg gatcgtagtg cacggagtct tccgcggcgc ccgtttccaa
     4981 ctgcacgccg aagcgcgacg cgagccgttg gttggcatcg ccgaccggga agtggtccac
     5041 gatgagcagg agcgcgccgc cgccggcgac ccagtcgcga acggcgtcgc attcggtttc
     5101 ctcgaaggcc ggctcgtcgt tgacgtcgtt gtcgcccagc gcggccgcga tcacgaatac
     5161 gtcgaagccg tcgagcgctt cggcccggat ctcgttcgtg ctctgcgcga ctcggtagcc
     5221 gtcggactcg atcagctcga cgaagggccg gtagctcttg taagcccggt ggacattgtg
     5281 gtggccttcg tcgaagagaa cacgcgggcc tgggccggag tacgcgggca ccgccacgtg
     5341 ggtgtcgaag tcgttgtcca ccttgtcacc ggaggaacag ctcgccacga cgacagtgga
     5401 aacgagcagc agggcaacgg gctggatact cgagcggata aacgcgggca tcggcacacc
     5461 tctggaatag gatcaccggg cggaccgtcc acccggttcc acgcattggg accggtgtgg
     5521 ggatccggtg gtttcaggtt tcgatggtgt gtcgtcttgg aggcagggag tttcagccgc
     5581 tcgcacgggc gcgacggcta gcgcggcagc gagggggcgt cgcccatgga cgaatcggcc
     5641 ggtgcgggct cggcgagggt ctcgtggtgc accaacgccc cgtactgctc gcgcacgtgg
     5701 tggtagtcgg tctcgacgga gtcgcgcagc gcgggccatt ctcgctcggg caccgcgctc
     5761 atcgcggcca ggcgggagcg caacgcctgc tggttccgct tgagatcctc cacttcgctc
     5821 gcgaaaacgc tgtcggtcag cgtgggggtg tcgcccaggc tcgcgatccc gcggtcgagc
     5881 tgccgcatct tgctctccat cacgatcgag aactgacggc gctcctggcg cttgagccgc
     5941 gccgcgtcgt cgcggctaca cgcaccgccg atgcacatcg ccccgccgga gagcagaact
     6001 ccggcgagca gcgcacccgc gggcagcgcg agaccccttc ctttcatgac cgaacccttt
     6061 ctgcgtcgcg tcagaccgcg ctcgcgcagc tcgtcgcttc gtacaaaccg tcctgatcgt
     6121 ggatgcacat gtagcgatcg tggatgccca gccatccgcg gcggcgaaac acgtttagcg
     6181 tgctgctggt cacttcgcgc gtcgcaccga tcatgctggc gatgtcctgc tgcgtgagca
     6241 gaacattgac cttggtaccg cagtgcagcg ccttcgggaa ctccttgcac aggcgcagga
     6301 ggatgcgcgc ggtgcgcgcg ggcacgcgcc ccgacatctc gtcgagacgg tcttccatct
     6361 gcgccatgcg cagcgatcga atgcggctga tctcggcctg cacgcgggcg ctgccgacga
     6421 tctgctccaa ctcgcgcacg cgcacgcgcg cgaccacgct ggactgcagc gtcgtgatgc
     6481 tgacgtcctc gcggtagtcg gagacgggaa cgccgtaggc ctcacccggt ttcatcatgt
     6541 acagcacggt ctcgccgccg ttgccgtcca agcgcgaaac cttggccagg ccgtccatca
     6601 agaaggaaag gttcccgggg tcgctctgca ggccgatccg ggtccgtttg tcgacggact
     6661 gaaaacgagt gatcccggcg acgcgggcca ggtcggagga cgtcacgtcc cggaagagtt
     6721 ccatggtccg aagcgaagcg cattcgacga gttgcgcgat ctcggggacc gcgacccgcc
     6781 gggtctggct ggtcggtgtg gctatcatca tggtcggctc ctttcaacgg ggctgcctca
     6841 tctcgagcaa gattgacccc aaggcggttg cctggctatt gaagtgatgg ttataatgtg
     6901 ctcaccccga cttcatgggt tcttcagacg ccgatcctgc cccgctgcct tacactcgag
     6961 gggggccacc cgtcatgcgc gtactcgttg ttgaagataa tccgaaaatg gccgaggcca
     7021 tccgcaaggg cctggtccag cacaactacg ccgtcgacgt gttcggccgc ggctacgagg
     7081 ccgaagaagc cgcagccggg gcggactacg acgccatcgt gctcgatttg atgctgcccg
     7141 accgcgacgg cgtcgacgtg tgtcgcaatt tgcgacgccg cggcatcgca acgcccatct
     7201 tgatgctgac cgccctggcc gacaccgaga gcaaggtcgc gggcctcgac gccggcgccg
     7261 acgactacct caccaagccc ttcgagttcg aggagctggt ggcgaggatt cggaccctga
     7321 tgcgccgcgg gcgggcggtg gaaagccggg tcctcaagta cggcgacttg gaattcgacc
     7381 tgaacgcgcg ccgcatcaca cgcgcgggcc gcgccatcca actctccggc aaggagacgg
     7441 cgttgctcga gtacttcatg cgcaaccccg accgcgtcgt cgcgcggcag accctagtcg
     7501 agaagatctg ggacaccagc tatcagccgg tgagcaatgc catcgacgtc cacatctcgt
     7561 ccctgcgcaa gaagatcgac cgcgacttcg acgaatcgtt cattcatacc gtggtcgggg
     7621 cgggctaccg gttcggtcgc acccacggcg atgtccccgg caagaccgcg actgacgccg
     7681 acacgaaatg accagcctcc cgctgcgact caagctcaca ctgtggttcg tgactctgtt
     7741 cgcgtccatc tatatgggcc tgatcggggt agtgtggttc atcggtcgca gctcgcaaaa
     7801 ccggctcttg gacgaagcgc tcatcacggt ggcgcgcggc gtgggaggac ttctggtcga
     7861 catcgagggc gacttctcga atctggactt gggtccctat cagcccgtcg accgcgagtt
     7921 cagcctgctc gccgtgcgcg acggcaacgg ggtcgtggtc gcgtccgact tgcgcgtcga
     7981 caccggtgcc ttgccgcctt tgccgccgcc cgatcaagag agcacgatca cgcggatcaa
     8041 cggcgatctg gcccgcagct tggtcggacg cgcgatccag actcgcctcg tcacctaccg
     8101 cgtttcctcg cgcgacggtg tcgtgcacta tatcgatctc gcgcgcgcaa ccgaattcgg
     8161 ccggcgccag cgccagctct tctccgacgt catcttcatc gcggggttgg ggggcgtgtt
     8221 ctcttcggcg ctggcggcgt ggttcttggt gggacgcgca accactccct tcgaaagact
     8281 ggccgaaatc gcgcggcgcg tgggtccgga gagcgagacg cgttttccct ccgggtcgag
     8341 cggccgcgag atcgagcgcc tgcagaccga gctcaacgag gccctggggc gcctggagga
     8401 aggctatcgc gtgcggcagc gcttcctcgc caacgtcgcc cacgacttga agacacccat
     8461 tagtgtcatg ctcacgcagt cgcaggtaat cgagccggaa gccgcctcgc tcgacgagtt
     8521 tcgactgtat cgcaacagca tgatcgagga gctgcggcgc ctgcgcggca tcatcgaggg
     8581 cattctcgcg ctctcgcgcg cgagccaggg cgaggcattg ctgcggcgct cgcaggtgcc
     8641 cgtcaacacg ctgctcgcgc agtcggcgct gcggtgcaag gcgcaggcgg acgccgcgca
     8701 ggtgcggctc aaggtcgttg agctcgacgg cgacgaaaac cgggagctgt tcggcgactt
     8761 cgaccttctg tgcacgatgc tcgacaacct gattcgcaac gcgattcgct tttcgccgcc
     8821 gcgcgagacg gttcacctgg aagcggagct cgccgacggc cgcgcgcgcc tctcggttcg
     8881 cgaccacggg cccggcatcc cggaggaata cctggatcgc atcttcgaac ggttcatcca
     8941 ggtgcccgac gaagacggta aagtgcgcgg cggcaccgga ctcgggctcg cgatcgccaa
     9001 gacggtggcc gaggcgcacg gaggcaccat cgaagcacgc aaccggcgtc gcggcgggtg
     9061 cgccttcacg atctcgttac cgttgagtgc gaccgctccc gcggcgaacg ccgcttctac
     9121 ccgcgtgtga ggtccttcac agcctcccgc cccgcttcgg ggtaacatcc cttcgaccaa
     9181 ccaagggagg ctcatcatga atcgcacgac cctcgtgctc gccgccgtcg tgatcttcgc
     9241 gacgaccggc tgttcgaaca agaaactcat cgcacaaaaa gacgcacaga tcgccgatct
     9301 gcaagccgac gtaaccgagt tgcaggccga agtcgccgaa cagaggcgta
//
LOCUS       DASPGK010000074         9391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1306502, whole genome shotgun sequence.
ACCESSION   DASPGK010000074 DASPGK010000000
VERSION     DASPGK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306502"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="VEC56_02020"
     CDS             complement(<1..1664)
                     /locus_tag="VEC56_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXV12960.1"
                     /translation="MCTNTSRRPRRSLRRARALAVLALLLSGLSAPASAQLEWTNHID
                     ASMVNDIVHKDGVLYMATFGGLLVYDVARGQFEQFDNVDGLPSNALNCLVFDPDDNLY
                     IGTADFGVAKVRISGGRPVLVRSLNAQIDGLSSNTVNSIAPWGADILYGANPGAGTIR
                     NDFASARYFERDGLPDDDVKDVLADGDYAWIATGAGVAVLDRFGLLRRPTGAPGVANV
                     LASDGATIWVGTNDGVWRMDPSDSSWTDVGPDTREMYGLLWDGTRMWGGGTRNFFRYT
                     GAGQNWDLFSTTPIASSYRFGAGGSINQIRGLAVASDGNVYLGSIHPTELRGVNVIRF
                     DGTTQVNLIPNAPGGNDVKRLSVDVDGSVWAMFAPFYVGKLMPSGAWVNYNTAIAGIE
                     VPSSQYTNLALLADSQGFKWFCSLPSPTLDRLDDRADANYGNDAWTRLALDSGGGDGL
                     GTLFLQRAHEDPVGNRWFLSNRGGIQILSRDGSEWFEMAQVKESRMLSDDVIDVAFGP
                     SSVYIAYRTLGVQRWTLRGFDWASITNTTDDAWGTPVPGTLLGGQV"
     gene            complement(1654..3831)
                     /locus_tag="VEC56_02025"
     CDS             complement(1654..3831)
                     /locus_tag="VEC56_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV12961.1"
                     /translation="MPRFEEGFHSIVRVSAIIVTYNSARVIGACLASLAPDVQSGLLE
                     VIVVDNASADGSPDLVRREFPWATVIAGRENLGYSKGVNVGIREARARYLFILNPDTV
                     VKRGAVTRLVDFMDQNPGAGIAGPKLVFQDGSVQLSCRRFYTFTVLLLRRTPLGKLFK
                     NPKPLREHLMLDFDHASTREVDWLLGAAMFVRREAVDSVGMMDERFFLYFEDVDWCYR
                     MKQRGLSVYYVADAVVEHGYKRESAQTVLNRSFVAHLASLVRYYEKWGAVAYLVKRYR
                     EVGKVLLFLALDVAAFNAAFFSAYYLRVLLADIFTNPIFAVSAYRRFIVFENLLFVFT
                     FAAAGLYRIRRETKSVDELFDIGRAIVLASVLLMTSTYLSQIRTYSRMVVVFLVPFAI
                     LYAWLLRSGVRRVHRRLLAQKVDLKRACVVGPKARAREVELKLTQDDAMGIDVVGVID
                     TAGQSDEILTGTLGPLENLERIVDRYRIQQIVVLPGSLSDEQLANTLALARRRVVDVV
                     VLTDYAGLVFQNARVTDLQGRPVILYPRDARYLAARFAKRALDVAAGAAFAVVSAPFY
                     VLYSLYAFSKARKPFVVGERLGARGESIAVPVAGNGQADGPSDFVNLPLFWLVVIGKM
                     SIVGPYPLPVADARHVDGVARFRYDARPGVTGPWRSGAGAIARDVLLVQDAAYVQNWS
                     LVDDAKTLVATFGRMLFGKRRVLEIANGGPRATSNELGGTHVH"
     gene            complement(3803..4522)
                     /locus_tag="VEC56_02030"
     CDS             complement(3803..4522)
                     /locus_tag="VEC56_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HXV12962.1"
                     /translation="MEALVVIPTYNERDNLERLVRALLALPVDLNVLVVDDNSPDGTG
                     RIADDVVAQEPRVHVLHRAGKLGLGTAYIAGFKWGLAHTDARFVFEMDADFSHDPAAI
                     PRFLERIRDADLVIGSRYVEGVTVVNWPLSRLILSVSANIYTRIVTGMPLHDATGGFK
                     CFRREVLMALPLDQIKSDGYSFQIEMNFHAWKRKFRIVEMPIVFTDRLVGASKMSRRI
                     IWEAAFMVWKLRFASLRRRIP"
     gene            complement(4504..7002)
                     /locus_tag="VEC56_02035"
     CDS             complement(4504..7002)
                     /locus_tag="VEC56_02035"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXV12963.1"
                     /translation="MVLLAVVAMLAGVGLLYPDIVFEGKIFVSGDSQAAASHAAVGNK
                     SLARGEYPVWNPYLFCGMPSFGSLSFTPWVYPINWVIKPLRAVLPLPEYTWLFVHTMV
                     TGFGVFLLLRDRGIHAAAAIGAGVLMMWMPNLVAVGANGHGSQACAVAYLPLALLFWD
                     RVWRGRNVVGNASALAVTLGFSMLRSHFQISYYTYALVVLHLVFFGGMALVDAARGRA
                     ASVSPLPARLGAKLRRDGTTPWGGALADTGFSSVVLAAVVGVSLLICAVLYLPVHDYA
                     KYSIRGASETGGLDYGYATNWSLHPSEMLTFLVPFSFGFGKDLYLGHMPFTDYPNYLG
                     VAVLAFAVVALLRVRTRWVAFLAFVTVVATLVSFGKFFPVLYDPLFKFAPYFSKFRVP
                     VMVLIVQQLAVILLFAIGLDHVLKSDRARLRRAALRGLVVAGACFVLALLSQGYWTDG
                     FASGAAARVRVTDDPATRLTVARMAGEFLARDLVQLAALALALAAAVFAFASSRLRPL
                     AFASVVLVVGLVDYYRVDRYILHPERFRHHDAYRIIRDRAAVDRYLQTDPVMEFLRSR
                     EGIFRILPIDDLRNATSTASFMSNRYMVFDIASIGGYQPAKLSVYDEFLRAFAFSLQE
                     GRLDLVHMLNARYVVTSARLPEQPSLTPVFVGDDYEGTARAIYENRDAFPRAWVVGEY
                     AVASREQSLEALASGRVDLRRSVLLTKEPSPKPAPGDSAEVTITRWGAREAAFAVELD
                     RPGIVVVSDAYYPDWKATVDGQPVEILQANHAFRAVALPAGRHDVVMRYDASVLERGA
                     AISASSLAVAVLAMVFSWLAGARREKRLWWKRSS"
     gene            complement(7153..8277)
                     /locus_tag="VEC56_02040"
     CDS             complement(7153..8277)
                     /locus_tag="VEC56_02040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV12964.1"
                     /translation="MRVCLVGPAYPYRGGISQFTHMLARELRRSHDVALVSFTRLYPA
                     FLFPGRTQFDERARTAGEASVRVIDSIDPRSWRRAARVVLEFRPDVVVFQWWQPFFAP
                     AMRSIAARVRAHSDARIVFLCHNVLPHEARPTDRAVVKWGLERAHGFVVQSREDGATL
                     SGLIPGARVVFNPMPVFDLFAGGGPTREAARAALGLSGRVILFFGLVRAYKGLDTLLD
                     AFAACGERLHATLLVVGEFYEDRGRYDERIRALGIEPRVRVIDEYVPDDAVASYFRAA
                     DVVVLPYVSATQSAVVQTAFSLDRPVIVTRVGGLPDIVEDGVTGYVVPPRSPEDLARA
                     ITRFFEEDAGDSMVREIRARAGRFSWARCVDAVLEAAGAR"
     gene            complement(8274..9236)
                     /locus_tag="VEC56_02045"
     CDS             complement(8274..9236)
                     /locus_tag="VEC56_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV12965.1"
                     /translation="MKISLVIPCYNEQDALPELFDRIEAVMAEMKCDYEYVFVDDGST
                     DRTIAVLRALRERSPRVGVISFRRNYGKSAALNEGFRAASGDLVVTIDADLQDDPAEI
                     PNLLKRIQAGADLVSGWKQNRQDPPSKTLPSRVFNRVTSIVTGVKLHDFNCGFKMYRR
                     EVVDAITVYGELHRFIPALAAWEGFRVDEVGVRHFKRKYGKSKYGSRRFLNGFFDLVT
                     TMFVTRRALNPLHLFGRVALVLFVLGLAPQIYFLVQWLAGEGLRVRPIMLLGFVLVIV
                     AIQVASIGLLAELISARSAREAVYAFKEYDVRGERAPRARARSA"
BASE COUNT         1728 a         3205 c         3124 g         1334 t
ORIGIN      
        1 aacacctgac cgcccaagag cgtgccgggg accggcgtcc cccacgcgtc gtcggtcgtg
       61 ttggtgatcg aggcccagtc gaaaccgcgc agggtccatc gctgcacgcc cagcgtacgg
      121 tacgcgatgt agaccgacga gggcccgaac gcgacgtcga tcacgtcgtc cgagagcatg
      181 cgcgactcct tgacctgcgc catctcgaac cactcgcttc cgtcccgcga gagaatctgg
      241 atcccgcccc ggttgctcaa gaaccagcgg ttgccgaccg ggtcttcgtg cgcgcgctgc
      301 aggaacagcg tccccaaccc gtcgccgccg cccgagtcga gcgccaagcg cgtccacgcg
      361 tcgttgccgt agttggcgtc ggcgcggtcg tcgaggcggt cgagggtcgg gctcggcagg
      421 gagcagaacc acttgaaccc ctgcgaatcc gcgagcagcg cgaggttggt gtattggctg
      481 ctcgggactt cgatccccgc gatggccgtg ttgtaattga cccaggcccc cgacggcatc
      541 agcttaccga cgtagaaggg agcgaacatc gcccacaccg acccgtcgac gtcgaccgac
      601 agccgcttga cgtcgtttcc gcccggcgcg ttcgggatca aattgacttg cgtggtcccg
      661 tcgaatcgga tcacgttgac gccgcgcagc tcggtaggat ggatgctccc gaggtagacg
      721 ttgccgtcgc tggcgacggc gagcccgcga atctggttga tggaaccgcc cgcgccgaat
      781 cggtagctgg acgcgatggg cgtggtcgag aagaggtccc aattctgccc ggcgccggtg
      841 tagcggaaga aattgcgcgt gcccccgccc cacatccggg tgccgtccca aagcagcccg
      901 tacatctcgc gcgtgtccgg accgacgtcg gtccacgacg aatcgctcgg gtccatgcgc
      961 cacacgccgt cgttggtgcc gacccagatg gtggcgccgt cgctggcgag cacgttggcg
     1021 acgccgggcg ctcccgtggg acggcgcagg agcccaaagc ggtcgagcac cgccacgccc
     1081 gcgccggtcg cgatccacgc gtagtcgccg tcggccagca cgtccttgac gtcgtcgtcg
     1141 gggagcccgt cgcgctcgaa atagcgcgcg gacgcgaagt cgttgcgaat ggtgcccgcg
     1201 cccgggttgg cgccgtacag gatatccgca ccccagggcg cgatcgagtt caccgtattg
     1261 ctcgagaggc cgtcgatctg cgcgttgagc gagcgcacca ggaccggcct ccctcccgag
     1321 attcgcacct tggcgacacc gaagtcagcg gtcccgatat agagattgtc gtcggggtcg
     1381 aacaccaggc aattgagtgc gttcgagggg agcccgtcga cgttgtcgaa ctgctcgaac
     1441 tggccgcggg cgacgtcgta caccaacagg ccgccgaagg tcgccatata gaggacgcca
     1501 tccttgtgga cgatgtcgtt caccatgctg gcgtcgatgt ggttggtcca ctcgagctgt
     1561 gccgatgcgg gcgcggagag cccgctcaaa aggagcgcga gcaccgcgag ggcgcgcgcg
     1621 cgtcgaagcg accgtcgggg gcggcgcgag gtgttagtgc acatgggtac cccccaattc
     1681 gttggacgtc gcgcgcggac cgccgttcgc gatctcgagt acgcgcctct tgccgaacag
     1741 catgcgaccg aacgtcgcca ccagggtctt ggcgtcgtcg acgagcgacc aattctggac
     1801 gtaggcggcg tcctggacca ggagaacgtc gcgagcgatc gcacccgccc cgcttcgcca
     1861 cgggccggtg acgccgggcc gcgcgtcgta gcgaaaacgc gcaaccccat cgacgtgacg
     1921 cgcgtccgcg acgggcaacg ggtacggtcc cacgatactc atctttccga tgacgaccag
     1981 ccagaacagg ggaaggttga cgaagtcgga cgggccgtcc gcttggccgt ttccggccac
     2041 gggcaccgcg atggactctc cgcgggcgcc gagccgctca ccgacgacga agggcttgcg
     2101 cgccttcgaa aacgcgtaga gcgagtataa cacatagaat ggagccgaga ccaccgcgaa
     2161 ggccgctccc gccgcgacgt cgagcgcccg cttggcgaag cgagccgcga ggtagcgcgc
     2221 gtcgcgcgga tacaggatca ccggccggcc ctgcaggtcg gtcacgcgcg cgttctggaa
     2281 caccaggccc gcgtagtcgg tgaggaccac cacgtccacg acccgccggc gcgcgagcgc
     2341 cagtgtgttc gcgagctgct cgtcgctgag cgagcccggc agcaccacga tctgctggat
     2401 gcggtagcgg tcgacgatgc gctcgagatt ctccagcggt cccagggtcc cggtcaggat
     2461 ctcgtcgctt tgcccggcgg tgtcgatcac gcccaccacg tcgatgccca tggcgtcgtc
     2521 ctgggtgagc ttgagttcga cctcgcgtgc ccgcgccttg ggccccacca cgcacgcgcg
     2581 cttcaagtcc accttctgcg ccagcaaacg ccggtgcacg cgccgcacgc cggagcgcag
     2641 gagccacgcg tacaggatcg cgaacggcac caggaacacc accaccatgc gcgagtaggt
     2701 tcgaatctgg ctcaggtacg tgctggtcat gagcagcacc gacgcgagca cgatggcgcg
     2761 gccgatgtcg aacagctcgt cgacggactt ggtctcgcgt cgaatccggt acagaccggc
     2821 ggccgcgaag gtgaacacga agagcaggtt ctcgaacacg atgaagcgcc ggtacgcgct
     2881 cacggcaaag atggggttgg tgaagatgtc ggccagcagc acgcgcaggt agtacgcgga
     2941 gaagaatgcg gcgttgaacg cggccacgtc gagggccagg aacaagagca ctttcccgac
     3001 ttcgcgatac cgcttcacca agtacgccac cgctccccac ttctcgtagt agcggaccag
     3061 gctggccagg tgcgcgacga aggagcggtt gagcacggtt tgcgcgctct cgcgcttata
     3121 cccgtgctcg accaccgcgt cggccacgta gtacaccgac agcccgcgct gcttcatccg
     3181 gtagcaccag tcgacgtcct cgaaatacag aaagaagcgc tcgtccatca tgcccacgct
     3241 gtcgacggcc tcgcggcgca cgaacatcgc cgcccccagc agccagtcga cttcgcgcgt
     3301 ggacgcgtgg tcgaaatcga gcatcaagtg ctcgcgcagc ggcttgggat tcttgaacag
     3361 cttgcccagc ggagtgcgcc gcaagagcag cacggtgaag gtatagaagc gacggcacga
     3421 gagttggacc gagccgtcct ggaacaccag cttgggtccc gcgatgcccg cgcccgggtt
     3481 ctggtccatg aagtcgacca gccgcgtgac cgcaccgcgc ttgaccacgg tgtcggggtt
     3541 caagatgaac aggtagcgcg cgcgcgcctc gcggatgccc acgttgacgc ccttcgagta
     3601 ccccaggttc tcgcgccccg cgatcacggt ggcccacgga aactcgcgcc gcaccagatc
     3661 cgggctcccg tccgcactcg cgttgtcgac cacgatgact tcgagcaggc ccgactggac
     3721 gtcgggggca agcgaagcga ggcacgcgcc gatcacgcgc gcgctgttgt aggtaacgat
     3781 gatggcggag acgcgcacga tgctatggaa tccttcttcg aagcgaggca aagcggagct
     3841 tccacaccat gaaagccgct tcccagatga ttcggcgcga catcttcgac gcgcccacga
     3901 ggcggtcggt gaagacgatg ggcatctcga cgatgcggaa cttccgcttc caggcgtgga
     3961 agttcatctc gatctggaac gagtagccgt cgctcttgat ctggtcgagc gggagcgcca
     4021 tgagcacctc gcgccggaag cacttgaaac cgccggtggc gtcgtggagc ggcatgcccg
     4081 tgacgatgcg cgtgtagata ttggccgaca cgctcaagat caggcgcgag agcggccagt
     4141 tgaccaccgt gacgccttcg acgtagcgcg atccgatcac caggtcggcg tcgcgaatgc
     4201 gctccaagaa gcgcgggatg gcggccgggt cgtgggagaa atccgcgtcc atctcgaaca
     4261 cgaagcgcgc gtcggtgtgg gcgagccccc acttgaaccc ggcgatgtac gccgtgccca
     4321 gccccagctt ccccgcgcgg tgcagcacgt gcacgcgggg ctcctgggcc accacgtcgt
     4381 ccgcgatgcg tccggtcccg tccggcgaat tgtcgtcgac caccagcacg ttcaggtcga
     4441 cgggcagcgc caacagggcg cgaaccagtc gctcgagatt gtcgcgctcg ttgtaggtcg
     4501 gaattacgac gagcgcttcc accacagcct cttttcgcga cgagcccccg ccagccaaga
     4561 gaacaccatc gccagcacgg cgaccgccag cgacgacgcc gaaatcgcgg cccctctctc
     4621 gagcaccgac gcgtcgtaac gcatgaccac gtcgtggcgg ccggccggca gtgccaccgc
     4681 gcgaaacgcg tggttggcct gcaggatctc gaccggctgg ccgtcgacgg tcgctttcca
     4741 atcgggatag taggcgtcac tcacgacgac gatcccgggg cgatcgagct cgaccgcgaa
     4801 ggccgcttcc cgggcgcccc agcgcgtgat cgtcacctcg gcgctgtcgc cgggcgcggg
     4861 cttgggcgag ggttccttgg tcaacaagac gctgcgtcgc aagtccacgc gtccgcttgc
     4921 cagcgcctcc agggactgct cgcgcgacgc gaccgcgtac tccccgacca cccacgcgcg
     4981 cgggaacgcg tcacggttct cgtagatcgc ccgcgcggtt ccctcgtagt cgtcgcccac
     5041 gaacaccggc gtcagcgacg gctgctccgg caggcgcgcg ctggtcacga cgtagcgcgc
     5101 gttgagcatg tggaccagat cgagacggcc ttcctggagc gagaacgcga acgcgcgcag
     5161 gaactcgtca tagaccgaaa gcttggccgg ctggtacccg ccgatcgacg cgatgtcgaa
     5221 caccatgtag cggttggaca tgaaagacgc ggtcgaggtc gcgttgcgca ggtcgtcgat
     5281 cggcaggata cggaatatcc cctcgcgact gcgcaggaac tccatcaccg ggtcggtttg
     5341 caggtagcga tcgaccgcgg cccggtcccg gatgatccga taggcgtcgt ggtggcggaa
     5401 tcgctccggg tgcaggatgt aacggtcgac gcggtagtag tcgaccaaac ccacgacgag
     5461 taccacgctc gcgaaggcga gcgggcgcaa gcgcgacgac gcgaacgcga acaccgccgc
     5521 ggccagcgcc agtgcgaggg ccgccagctg gaccaggtcg cgcgccaaga actcgccggc
     5581 catgcgcgcg accgtcaggc gcgtggcggg gtcgtcggtc acgcgcacgc gcgcggccgc
     5641 gccgctcgcg aagccgtcgg tccagtaccc ttgcgacagc agggccagca cgaagcacgc
     5701 ccccgcgacc accagcccgc gcagcgccgc gcgtcgcaag cgcgcgcggt cgctcttgag
     5761 cacgtggtcg agtccgatcg cgaacaggag tatcaccgcg agctgctgga cgatgagcac
     5821 catgacgggc acgcggaact tgctgaagta gggcgcgaac ttgaagagcg ggtcgtacaa
     5881 cacgggaaag aacttcccaa acgagaccaa cgtcgcgacc accgtcacga aggccaggaa
     5941 ggcgacccat cgcgtgcgca cgcgcagcag tgcgaccacc gcgaacgcca gcacggccac
     6001 accgaggtag ttggggtagt ccgtgaaggg catgtgcccc agatagagat ccttgccgaa
     6061 cccgaacgag aacgggacca ggaaagtcag catctccgac gggtgcaggc tccagttggt
     6121 ggcgtacccg taatcgagcc cgccggtctc ggatgccccg cggatcgagt acttcgcgta
     6181 gtcgtgcacc gggaggtaga gcaccgcaca tataaggagc gagacgccca ccacggccgc
     6241 caagaccacc gacgaaaagc cggtgtcggc cagcgcgccc ccccacggcg tggtcccgtc
     6301 gcgacgcaac ttggccccga gcctcgccgg gagcggggag accgacgccg cccttccccg
     6361 cgccgcgtcg acgagcgcca tcccgccgaa aaagaccagg tggagcacca ccagcgcgta
     6421 tgtgtagtac gaaatttgga aatgactgcg cagcatcgaa aagccgagcg tcacggccag
     6481 cgcggaggcg ttgccgacca cgttgcgccc gcgccagacg cgatcccaga acaagagcgc
     6541 caacggcagg tacgccacgg cgcaggcctg gctcccgtga ccgttggccc ccaccgccac
     6601 caggttcggc atccacatca tgagcacgcc cgcgccgatc gcggccgccg cgtggattcc
     6661 acgatcgcgc agcaacagga acaccccgaa gccggtgacc atcgtatgaa cgaagagcca
     6721 cgtgtactcg ggaagcggga gcacggcgcg cagcggcttg atcacccagt tgatgggata
     6781 cacccagggc gtgaacgaca agctcccgaa gctcggcatg ccacagaaaa ggtagggatt
     6841 ccagaccggg tattcgccgc gagcgagcga cttgttcccc accgctgcgt ggctggcagc
     6901 ggcctggctg tcgcccgaca cgaagatctt cccctcgaag acgatgtccg ggtagagcag
     6961 cccgacgccc gccagcatcg cgacgacggc gagcagcacc acgattcccg gcgatgagaa
     7021 tcgcgcgcgc gcgtgcgcgg gcttcgcggt gccggaggct gcggatttgc gggaagactt
     7081 cgaggacata aggaatgagg tggtcgccga atgataccac aggccgcacg gggcgtgaat
     7141 cgccccgccg tgctagcggg cccccgccgc ctccaagacc gcatcgacgc atcgcgccca
     7201 cgaaaaccgg cccgcgcgcg cgcgaatctc tctcaccatg gaatccccgg cgtcctcctc
     7261 gaagaagcgc gtgatcgcgc gcgcgaggtc ctcgggcgac cgcggcggca cgacgtagcc
     7321 ggtgaccccg tcctccacga tgtccggcag gccccccacg cgggtcacga tcacggggcg
     7381 gtcgaggctg aacgcggtct gtaccaccgc actctgggtg gcggagacgt acggcagcac
     7441 gaccacgtcg gcggcgcgga aatagctcgc caccgcgtcg tcgggtacgt attcatcgat
     7501 cacgcgcacg cgcggctcga tgccgagcgc ccgaatgcgc tcgtcgtagc gcccgcgatc
     7561 ctcgtagaac tcgcccacca cgagcaaggt cgcgtgcagg cgctcgccgc acgccgcgaa
     7621 ggcgtcgagc agggtgtcga gccccttgta cgcgcgcacc agcccgaaga acaggatcac
     7681 gcggccggag agcccgagtg cggcgcgcgc cgcctcgcgc gttggaccac cacccgcgaa
     7741 caagtcgaac accggcatcg ggttgaacac gacgcgcgcg ccggggatca gccccgagag
     7801 cgtcgcgccg tcctcgcgcg actgcacgac gaacccgtgc gcgcgctcca ggccccactt
     7861 gaccacggcc cggtcggtcg gccgcgcctc gtgcggcagc acgttgtggc acagaaatac
     7921 gatgcgcgcg tcggagtgcg cccgcacccg cgccgcgatc gatcgcatgg cgggcgcgaa
     7981 aaacggctgc caccattgga acacgaccac gtcgggacga aactcgagca cgacgcgcgc
     8041 cgcgcgccgc cacgaacgcg ggtcgatcga atcgatgacg cgcacggacg cctcgcccgc
     8101 ggtgcgggcc cgctcgtcga actgggtgcg gccgggaaac agaaacgcgg ggtacaggcg
     8161 cgtgaaactg accagcgcga cgtcgtgcga gcgtcggagc tcgcgcgcga gcatgtgcgt
     8221 gaactggctg atgcccccgc gatacgggta cgcggggccg accaagcaga ctctcatgcg
     8281 ctgcgcgcgc gcgcacgcgg cgctcgctcg ccccgcacgt cgtactcctt gaaggcgtac
     8341 acggcctcgc gcgcggagcg cgcggagatg agctccgcca aaaggccgat cgacgccacc
     8401 tggatcgcga cgatgacgag cacgaagccc aggagcatga tcgggcgcac gcgcaggccc
     8461 tccccggcga gccactgcac gaggaagtag atctggggcg ccaggcccag cacgaatagg
     8521 accagcgcca cccggccgaa cagatgcaag gggttgagcg cgcgccgcgt cacgaacatg
     8581 gtggtgacga ggtcgaagaa gccgttcaga aagcggcggc ttccgtactt gctcttgccg
     8641 tacttgcgct tgaaatggcg cacgccgacc tcgtcgacgc ggaatccttc ccacgccgcc
     8701 agcgcgggga tgaagcggtg cagctcgccg tacacggtga tggcatcgac cacttcacgc
     8761 cggtacatct tgaatccgca gttgaagtcg tgcagtttca ccccggtgac gatcgaggtg
     8821 acacgattga acacgcgcga cggcagcgtt ttcgagggcg ggtcctgccg gttctgcttc
     8881 caccccgaca ccaagtccgc gcccgcctgg atgcgtttga ggagattggg gatttcggcc
     8941 gggtcgtctt gcaggtcggc gtcgatggtg acgaccaaat cgccggacgc ggcccgaaag
     9001 ccctcgttga gcgcggccga cttgccgtag ttgcgccgga agctgatcac gcccacccgc
     9061 ggggagcgtt cgcggagcgc ccgcagcacg gcgatggtgc gatcggtgct accgtcgtcg
     9121 acgaagacgt actcgtagtc gcacttcatc tccgccatga ccgcctcgat gcggtcgaag
     9181 agctcgggca gcgcatcctg ctcgttgtag cacgggatca cgagggagat cttcacggcg
     9241 gagaggctaa ccctttccga ggcggcggcc aagcgattcc agccactcgg cctcgcgcgc
     9301 gagcccggcc tccaaaaccg tcttgggcgc gtaggccagc tcgcgggcgg ccagcgaaac
     9361 gtccgcgtag gtgtgcatga cgtcgccctt c
//
LOCUS       DASPGK010000075         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1190057, whole genome shotgun sequence.
ACCESSION   DASPGK010000075 DASPGK010000000
VERSION     DASPGK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190057"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1137
                     /gene="rsmA"
                     /locus_tag="VEC56_02050"
     CDS             310..1137
                     /gene="rsmA"
                     /locus_tag="VEC56_02050"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXV12966.1"
                     /translation="MKDGRFRPKKRFGQNFLHDRGTAAKIVDAACIGPGDLVVELGPG
                     RGVLTRLIAERGARLVAIELDRGLVAELSAEFAGRPDVEVISGDFTKISLAALASERG
                     FKRCILIGNIPYHLTRDVLFDFLVDEYESIAHAVVMMQREVGDRIASRPGSRTYGIPS
                     VVLQSLYDIEIVSKVAAGAFFPPPKVASIVLSFRPLAHPLLAEGDMRPFIVLVKNVFQ
                     QRRKTVYSTLRSFYSLSEGALARVGAETGVDLQSRPEALSKEAFLLLSRSVSAIRAS"
     gene            1168..>2409
                     /locus_tag="VEC56_02055"
     CDS             1168..>2409
                     /locus_tag="VEC56_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12967.1"
                     /translation="MISTAVAMLAAAAWVDAPTIVRAEQTSPADTTQARSLADFAQQR
                     AQSGFTLPDTLATSPRIGIRFTPTYINQVTGDVSSVGMRNVFQTNMTTPFGSVFNFNV
                     SAEEKHYRLQNKRDENKQLGATVAHTFNIFTSGSIGFIDSRVFNRSIIPGGAVQDYIF
                     NDQSVNASGSYKRNYLLHSTPLRSVRFDALVNGAAVQGERTYKNDETLAAGGFAGLAA
                     SLRSGEINVRGRGGHRETWDRSTTTLSEFDGLGSSEDSLSTGLVAEVGDSIFVDANYV
                     YYEAARRWADQAQGSLGGQQGGVENVFEETERRSSRATTLSLNARVWRGFAVRITGSH
                     DSQLYDYQIQKTRYSNTVADGLRGTVEYTAPWRTRAIVTLENTKTLRDFGPLSVSSYD
                     DTRYKAALGLSHRFKSALSLEL"
BASE COUNT          437 a          760 c          780 g          432 t
ORIGIN      
        1 cgttgaccct ggacgagttg tatatgctcc tcgaggacaa cgtgtgcgac ccggccccct
       61 ttgtctcgac gttcggggtg aagctggaca gctacgagga caagattcct tcgctcttgc
      121 cgagggtcca agagctggcg gcctgaaacc gccttgacgc cccctggaga aagtggctat
      181 actaggcctc aatctcgcgc ccggtccccg aaggcgcgac caggaggttc ctctctcact
      241 tctgctatcg atcgatcgtc atccccctgg cgaccgcgcg aagttcgcgc gaatctaacg
      301 agtagtcctt tgaaagacgg ccgctttcgc cccaagaagc gcttcggcca gaacttcctt
      361 cacgaccgcg gcaccgccgc gaagatcgtc gacgccgcgt gtatcgggcc gggcgatctc
      421 gtcgtggagc tgggtcctgg acgaggcgtg ctgacgcggc tcatcgccga gcgcggcgcg
      481 cgcctggtcg cgatcgaact ggatcgcggg ctggtcgccg agttgagcgc cgagttcgcg
      541 ggccgtcccg acgtcgaagt gatttccggc gatttcacga agatctcgct ggcggcgctc
      601 gcgagcgagc ggggattcaa acgatgcatt ctaatcggca acattccgta tcacctgacg
      661 agggacgtgc tgttcgattt cctcgtcgac gagtacgaat cgattgcgca cgccgtggtc
      721 atgatgcagc gcgaggtggg cgaccggatc gcgtcgcggc cggggagccg tacctacggc
      781 atcccgagcg tggtgctcca gagcctgtac gacatcgaga tcgtatccaa ggtggcggcg
      841 ggcgcgttct ttccgccccc caaggtggca tcgatcgtgc tctcgtttcg accgctggcg
      901 cacccgttgc tcgccgaggg cgacatgcgc ccgttcatcg tgctcgtgaa gaacgtgttt
      961 cagcagcggc gcaagaccgt ctacagcacc ctccgctcgt tctattcgct ctcggagggt
     1021 gccttggcgc gcgtcggcgc ggagaccgga gtcgacctgc aatcgcggcc cgaagcgctg
     1081 tcgaaggaag cattccttct gttgtcgcgc tcggtgtcgg cgattcgcgc ttcgtgaggt
     1141 tggagtctcg tgcgagttgg gcgcggcatg atctcgacgg cggtggcaat gctcgcggcc
     1201 gccgcgtggg tcgacgcgcc gacgatcgtg cgcgcggagc aaacctcccc ggccgatacc
     1261 acgcaagccc ggtcgctggc ggatttcgcg cagcagcgcg cgcagagcgg tttcacgctt
     1321 cccgacacgc tggcgacctc gccgcgcatc ggaattcgct tcacgccgac gtacatcaat
     1381 caagtcaccg gtgacgtctc gagtgtcgga atgagaaacg tctttcaaac caatatgacg
     1441 acgcccttcg gctcggtgtt caatttcaac gtctccgccg aggagaagca ctatcgcttg
     1501 cagaacaagc gggacgagaa caagcaactg ggcgcgacgg tggcgcacac gttcaatatc
     1561 ttcacgagcg gctcgatcgg cttcatcgac agccgcgtgt tcaaccggtc catcattccc
     1621 ggcggcgcgg tgcaggacta catcttcaac gaccagtcgg tcaacgcgag cggctcctac
     1681 aaacgaaatt acctgcttca ttcgactccc ctcaggagcg tgcgattcga cgcgctggtc
     1741 aacggcgcgg ccgtgcaagg cgagaggacg tacaagaacg acgagacgct ggcggcgggc
     1801 ggtttcgcgg ggctcgcggc cagccttcgc agcggggaaa taaacgtcag gggacgcggc
     1861 ggccaccgtg agacctggga tcggtccacg accacgctct cggagttcga cgggctgggc
     1921 tccagcgagg actcgctgtc gacgggcttg gtcgcggaag tcggcgacag catctttgtc
     1981 gacgcgaact acgtgtacta cgaagccgcg cgcaggtggg ccgaccaggc gcaaggatcc
     2041 ttgggagggc agcaaggcgg cgtcgagaac gtgttcgagg aaaccgaacg gcgcagctcg
     2101 cgcgcgacca ccctgtcttt gaacgccagg gtgtggcgtg gattcgccgt ccggatcacg
     2161 ggcagtcacg acagccagct ctacgactac cagatccaaa agacgcggta cagcaacacc
     2221 gtcgccgacg ggttgcgagg tacggtggaa tacaccgcgc cctggaggac gagggccatc
     2281 gtgaccctcg agaacacgaa gacgctccgc gacttcggcc ccctgtcggt cagcagctac
     2341 gacgataccc gctataaggc cgcgctgggg ttgtcgcacc gcttcaagtc ggcgctgtcg
     2401 ctggagctt
//
LOCUS       DASPGK010000076         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_370205, whole genome shotgun sequence.
ACCESSION   DASPGK010000076 DASPGK010000000
VERSION     DASPGK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370205"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1373
                     /locus_tag="VEC56_02060"
     CDS             222..1373
                     /locus_tag="VEC56_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXV12968.1"
                     /translation="MAGCAAALRDAFVAACRARHLDAHDHVLSLSGGIDSRAVGAGMR
                     AAFGTFSAVTFVAPGSSHADERDVASRVARALGADWRAYEFDHTDPSSIDGIIRLKLG
                     LNPVDVAFGLDYVRRVQADHAQPVALWTGEGADKMLCEHRAIPRRPNTDELVRFIVEK
                     NAVFAPARVRALTGVHEDDLLESIRAAVLSAGDVEPDDAYVHFLLAERVVRWHTEGED
                     RHRTSVWPIAPYFARDFFELARAIPAQWKHGRRVYRAFLEALAPDVAALPLAGGHAAP
                     ASTRFALEYALRETLRNNRYASSAYAHLRRRRASARTHNDPWRDRLAALREASGAPAC
                     FDAAAIDDVLSGRAPASSYAMAVLLTTVLAVRHIEKDRPKGGRDGMPAC"
     gene            1383..1784
                     /locus_tag="VEC56_02065"
     CDS             1383..1784
                     /locus_tag="VEC56_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXV12969.1"
                     /translation="MIREEEPRSRGERMTAGLGIIASIKEKLADALGATRSRVDVFQA
                     DVEHRLFRLLAMLIWGVVAFVSLSLGLLLAMLTVLFGFDLPPKYAFGIPALVFLAIGA
                     VAVIMLRVKKASKYDREKHAKRSPHATQTKV"
     gene            complement(1781..1933)
                     /locus_tag="VEC56_02070"
     CDS             complement(1781..1933)
                     /locus_tag="VEC56_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12970.1"
                     /translation="MARKVEVSGITGPIWFMGWLFTIGYVHLSFWKAVLALVIWPYYL
                     GATLSL"
     gene            complement(1939..2853)
                     /locus_tag="VEC56_02075"
     CDS             complement(1939..2853)
                     /locus_tag="VEC56_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXV12971.1"
                     /translation="MRQLALIAVLLIAPASALAQPAEQAPCWHAEFDAFSFLPGEWTV
                     HAEDRLGNGTWETTDARCWVTKELRGCMLSVRYFGSRAGGPFEARGYYGFDSIQGKLQ
                     QVWIDGEHGMLVTYAGSKVGDAIVLDYPFVLRGQPTILRAVYSEISKDAFHLEDSRSS
                     DGGKTWDVTSRYAFTRVPAVETKPLPSIELPKDLARVLTDYETGWRNKDEKALASLFT
                     EDGFVLSSGNPPVRGRAAIEKHYQESGGALSLRALEYATNGDTGFIIGAYTYEGQKGD
                     VGKFTLTLAKGKGGRWLIVSDMDNSNRR"
     gene            complement(2850..>3299)
                     /locus_tag="VEC56_02080"
     CDS             complement(2850..>3299)
                     /locus_tag="VEC56_02080"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HXV12972.1"
                     /translation="DRAATTILVVRHAEKPGQADSLSAAGFARANALVHVAGRANLKA
                     IYHSDTTRNRLTAAPCAKAFGLTPTAYPAKETDALVARIFSEHEGETVLVVGHSNTVA
                     KIVAAAGGPTIDNLAEDEYDTLFVVVVPPCRRGPATLAILQYGEESP"
BASE COUNT          517 a         1106 c         1101 g          575 t
ORIGIN      
        1 gcgaccaagc cttcgtactc gcgcacctcg atcgacctac gatcgatcgc gtggcgctcg
       61 cacagctctt gctcttcgga tatccgctcc gcgcgcggac tctcatcgaa ggcgtccatc
      121 gcctcctccc cggcgaggtg atcgtcgcct cagcgcgcgg ctgtagagtc gtcgcgccct
      181 cggcgtctcc gtttcgacga agctcgggcg cacccacgac gatggcgggc tgcgccgcgg
      241 cgctccgcga cgccttcgtg gccgcgtgcc gcgcgcggca tctcgacgcg cacgaccacg
      301 tcctctcgct gagcggcggg atcgattcgc gcgcggtggg tgcgggcatg cgcgcggcgt
      361 tcgggacatt ctcggcggtg acgttcgtcg caccgggttc gtcgcacgcg gacgagcgcg
      421 acgtggcctc gcgcgtagcg cgtgcgctcg gcgccgactg gcgcgcgtac gagttcgacc
      481 acacggatcc gtcctcgatc gacggcatca ttcgcttgaa actcgggctc aacccggtcg
      541 acgtcgcctt cgggctcgac tacgtgcgcc gggtgcaagc cgaccatgcg cagccggtgg
      601 cactatggac cggcgagggt gcggacaaga tgctgtgcga gcaccgcgcg atcccgcgtc
      661 ggccgaacac cgacgagttg gtgcgcttca tcgtcgaaaa gaatgcggtc tttgctcccg
      721 cgcgcgtgcg cgcgctgacc ggcgtgcacg aggacgacct gctcgaatcg attcgtgcgg
      781 ccgtgctatc ggccggcgac gtcgagcccg acgacgccta cgtgcacttc ttgctggcgg
      841 agcgtgtcgt gcgatggcac acggagggcg aggaccggca tcgcacgagc gtgtggccaa
      901 tcgcgcccta ctttgcgcgc gacttcttcg agctggcacg cgcgattccc gcgcagtgga
      961 aacacggccg acgggtctat cgcgcgttcc tcgaggcact cgcgccggac gtagccgcac
     1021 tcccgctcgc gggcggtcac gcggcaccgg catcgacgcg cttcgccctc gagtacgcgc
     1081 taagagagac gcttcgcaat aaccgttacg cgtcgagcgc atacgcgcac ctgcggcgcc
     1141 ggcgcgcgtc ggcgcgaacg cacaatgacc cgtggcgcga ccgcctcgcg gccctgcgtg
     1201 aggcgagcgg cgcgcccgca tgcttcgacg cggcggcgat cgacgacgtg ctttcgggcc
     1261 gcgcgccggc ctcgtcgtac gcgatggcag tactcctcac caccgtgttg gcggttcggc
     1321 acatcgaaaa ggaccgacca aagggggggc gtgacgggat gccggcgtgc taggatgtgc
     1381 gcatgatcag agaggaagaa ccgcgttctc gcggcgagcg aatgacggcc ggcctgggga
     1441 tcatcgcgtc catcaaagag aaactcgccg acgccctggg ggcgacgcgc tcgcgcgtcg
     1501 acgtgtttca ggccgacgtc gagcaccgcc tgtttcgatt gctggccatg ttgatctggg
     1561 gagtggtcgc gttcgtgagc ctgtcgctgg ggctcctgct cgcgatgctc accgtgttgt
     1621 tcggcttcga tctgccgccc aagtacgcgt tcgggattcc cgcgctggtg tttctcgcga
     1681 tcggtgcggt cgcggtcatc atgctgcgcg tgaagaaggc gtcgaagtac gaccgcgaga
     1741 agcacgccaa acgctcaccc cacgcgacgc agaccaaagt ctagaggctc agcgtcgctc
     1801 ccaagtagta gggccagatc acgagcgcga gcacggcctt ccagaacgag aggtgcacgt
     1861 agccgatcgt gaagagccag cccatgaacc agatcggtcc cgtgatcccc gagacttcca
     1921 cttttcgagc cattccgatt acctcctatt cgagttgtcc atgtcggaca cgatgagcca
     1981 gcggccgccc ttccccttcg cgagcgtcag cgtgaacttg ccaacgtcgc ccttttgtcc
     2041 ttcgtacgtg taggcgccga tgatgaagcc ggtgtcaccg ttcgtcgcat actcgagcgc
     2101 gcgcagcgac agcgccccac ccgactcctg gtagtgcttt tcgatggcgg cgcggcctcg
     2161 cacgggcgga ttcccgctcg agagcacgaa accgtcctcg gtgaagaggc tcgcgagcgc
     2221 cttctcgtcc ttgttccgcc agccggtctc gtaatcggtc aggacacgcg cgagatcctt
     2281 gggcaattcg atcgacggaa gcggtttggt ctcgaccgcg ggcacgcggg tgaacgcgta
     2341 cctggacgtg acgtcccacg tcttgccgcc gtcggaggag cggctgtcct ccaggtgaaa
     2401 cgcgtccttc gagatctcgg agtacacggc gcgcaggatg gtgggctggc cgcggagcac
     2461 gaacgggtaa tcgagcacga tagcgtcgcc gactttcgac cccgcgtagg tcactagcat
     2521 gccgtgctcg ccgtcgatcc acacctgctg gagttttcct tggatggaat cgaagccgta
     2581 gtatccgcgc gcctcgaacg gcccgcccgc gcgggatccg aagtagcgaa ccgaaagcat
     2641 gcaccccctc aactccttcg tcacccagca gcgtgcgtcc gtcgtctccc aggtgccgtt
     2701 gccgaggcgg tcctccgcgt gcacggtcca ctcccccggc aaaaacgaga aggcgtcgaa
     2761 ctcggcgtgc cagcagggcg cctgctcggc gggttgggcg agcgccgacg ccggcgcaat
     2821 caacaagaca gcgatcagtg cgagttgtct catggcgatt cttctccata ttgaaggatg
     2881 gccagggtcg ccggcccgcg acgacacggc ggcaccacga ccacgaacag cgtgtcgtac
     2941 tcgtcttcgg cgaggttgtc gatggtcggg ccgcccgcgg cggcgacgat cttcgcgacc
     3001 gtgttgctgt ggccgaccac cagcacggtc tcgccttcgt gctcggagaa gatacgtgca
     3061 accagcgcgt cggtttcctt ggcgggatac gcggtgggcg ttaggccaaa tgctttggca
     3121 cacggcgcgg cggtcaggcg attgcgcgtg gtgtcgctgt gatagatcgc cttgaggttg
     3181 gcccggcccg ccacgtgtac cagcgcgttc gcgcgcgcga agccggctgc cgagagcgag
     3241 tcggcctggc ccggtttctc cgcgtggcgc acgaccagga tggtcgtggc cgcgcgatc
//
LOCUS       DASPGK010000077         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1659408, whole genome shotgun sequence.
ACCESSION   DASPGK010000077 DASPGK010000000
VERSION     DASPGK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659408"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="VEC56_02085"
     CDS             <1..2081
                     /locus_tag="VEC56_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12973.1"
                     /translation="WAFGCVLYEMLAGKKAFDGETVTDVLAAVVTRAPDFAALPAGTP
                     RRLHALIERCLEKDPRKRLRDAGEARIALEDIVAHPVEAPAPATAPPPSAKRPAWRRL
                     APWAVALAAIVFAARATIRVENFREPTFVSVTLPRADIADAGMENLLMDLSRDGRTLA
                     YCARRDGVVRLYTRSFNEREAVELAGTENASNPFFSPDGQWIAFAADAKLKKVSVRGG
                     PVFEIRDLYEGHRAGAWLDDGSIICVPSFASPVFRIPPGGGIGTAVTVIDSAAGERTH
                     RWPGVFPGDEWVYFTVGTARNPGDYENAEIAVVSLKTGERRSLIKGASMARYAKPGHM
                     LFSKGGVLMSAPVDPDHPKLLGPAVPVLDKIAGEQTSGAVHMAMSGNGTLAFVTAREG
                     EYDMRLAWVDRTGRVEVLAAPPRRYTAPRVSSDGKQVLVTVGPAFGRGDVWRYDVERE
                     TLTRVTFDDKSVIAMWMPDQRHMIFSREDGPFSVMLASIQGEAKERSLFARALPAYIS
                     DISPDGEWVVISDWGAPDSDISVLPMGGGEPTPVVTDPFGQFGGIVSPDGKWIAYTSS
                     ETGVYEIHMRPFRRPGNRIQVSSGGGIHAMWGPNSDEICYLNGQSMMSVAVDVQADAV
                     NVGRPRKLFDLQHNARLQVDYRDFDMTKDGTRFIATYVANPELARRRIDVLLNANELL
                     ERAEKKGVAE"
     gene            2124..>4574
                     /locus_tag="VEC56_02090"
     CDS             2124..>4574
                     /locus_tag="VEC56_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV12974.1"
                     /translation="MTSWIGKSIAHYRVTTQIGAGGMGEVYRASDSRLGRDVAIKVLP
                     EAFAAEPERMARFEREAKVLASLSHANIAAIFGLEESNGARALVMELVDGTTLAERIA
                     KGALPVDEALPIAKQIAEALEYAHERSIIHRDLKPTNIKLSGDGVKVLDFGLAKALSD
                     DATASNSDLSKSPTLTAASTKLGVILGTAAYMSPEQAKGKAVDRRADIWAFGAVLFEM
                     LTGRQAFAGETVSETLASVMKDQPDWDALPASTPARVRDVLRRCLVKDPKQRLRDIGD
                     ARIRIEEELSGAPDTASATATTAGAKRRGIPAAPVAIVALVAAAIGVAAGFVLRKPAV
                     PPMFRATLTFPEEMRLDEENASIVISPDGRTVAVAASAKDHPQQLWLRRFDSDATTPI
                     AGTNGATYPFWSPDGRFVGFFADRKLKRVPTNGGTVQTICDAVDARGGSWSEHGMIIF
                     SPGPLTGLEIVPASGGTPQPLTTLQKNGETHRLPRFLPGGKRVLFMVGSADLSPGHVE
                     CLDIESKERTPVMQCRSEARYLGTGHLAFMIDDNLVVQRFDASSLKLAGEPVPVAESV
                     QFNIYRYTGGWDISEHGAFAYTTGVGVENGQLTWFDLEGRELGTLGTPNPIVGLNISP
                     DAQRVVTSERADQFDLWIWDVARGVHTCLTFGPEPAASPIWSRDGRTIYFGTGAGEVL
                     AKSADGASAATTVYKLPDTWLVPESMAPNGKEMLLSTQLRETGTDILVMTMVDSTAPR
                     KLFASPANEDDARVSPDGRWVLVTSDESGQPEVYVYRYPSLSGRWQVSSDGGVNGEWL
                     PNGRAILYRAPDQRLIQVD"
BASE COUNT          767 a         1504 c         1550 g          753 t
ORIGIN      
        1 tctgggcctt cgggtgcgtg ctctacgaga tgctcgcggg caagaaggcc ttcgacggcg
       61 agacggtgac cgacgtgctg gcggccgtgg tgacgcgcgc gcccgacttt gccgcgctgc
      121 cggcgggcac gccgcgccgt ctgcacgcgc tgatcgagcg ctgcctcgag aaggacccgc
      181 gcaagcggct gcgggatgcg ggcgaagccc gcattgcctt ggaagacatc gtcgcgcacc
      241 ccgtcgaagc gcccgctcca gcaaccgcgc cgccgccgtc ggcgaagcgg ccggcgtggc
      301 gcagactcgc gccgtgggcc gtggcactcg ccgcgatcgt cttcgccgcg cgcgcgacca
      361 ttcgcgtcga gaacttcagg gagcccacct tcgtgagcgt caccctcccg cgcgccgaca
      421 tcgccgacgc cgggatggag aatctgctga tggacctctc gcgcgacggg cgcacgctcg
      481 cgtactgcgc gcgccgcgac ggcgtggtgc ggctgtacac gcgctccttc aacgagcgcg
      541 aagcggtcga gctggccgga accgagaacg cgtccaaccc gttcttctcg cccgatgggc
      601 agtggatcgc gtttgccgcg gacgcgaagc tcaagaaggt gtccgtgcgc ggcgggccgg
      661 tgttcgagat tcgcgacctg tacgaaggcc accgcgccgg cgcgtggctc gacgacggct
      721 caatcatctg tgtgccgagc tttgcctcgc cggtgtttcg catcccgccg gggggcggga
      781 tcgggaccgc ggtcacggtg atcgatagcg cggcgggcga gcggacgcat cggtggccgg
      841 gcgtgttccc cggcgacgag tgggtctact tcacggtcgg taccgcgcgc aacccgggcg
      901 attacgagaa cgccgagatc gcggtggtgt cgctcaagac gggagagcgt cgcagcctga
      961 tcaagggagc gagcatggcg cgctacgcga aacccggtca catgctgttc tcgaagggcg
     1021 gcgttctgat gtcggcgccg gtcgaccccg accatccgaa gttgctcggc cccgccgtcc
     1081 ccgtgctcga caagatcgcc ggcgagcaga ccagcggcgc ggttcacatg gcgatgtcgg
     1141 gcaacggcac gctcgcgttc gtcaccgcgc gcgaggggga atatgatatg cgcctggcgt
     1201 gggtggatcg cacgggccgg gtcgaggtgc tggccgcgcc accccgtcgc tacaccgcac
     1261 cgcgggtttc gagcgacggt aagcaagttc tagtgacggt ggggcccgcg ttcgggcgcg
     1321 gcgacgtgtg gcgttacgac gtggagcgcg agacgctcac gcgcgtcacc ttcgacgaca
     1381 agtcggtcat cgcgatgtgg atgcccgacc agcgccacat gatcttcagc cgtgaggacg
     1441 gtcccttcag cgtgatgctg gcatcgatac aaggcgaggc caaggagcgc tcactcttcg
     1501 cgcgcgcgct gcccgcctac atctccgaca tcagtcccga cggcgaatgg gtcgtgatca
     1561 gcgattgggg cgcgcccgac tccgatatct cggtgctgcc gatgggcggc ggcgagccga
     1621 ccccggtggt gacggatccg ttcgggcagt tcggcggcat cgtctcgccg gacgggaagt
     1681 ggatcgcgta cacgtcgagc gagaccggcg tctacgaaat ccatatgcgg ccgttccggc
     1741 ggccgggcaa tcgcatccag gtgtcctcgg ggggcgggat ccacgccatg tgggggccga
     1801 attcggacga aatctgctat ctcaacgggc agtccatgat gtccgtcgcc gtcgacgtgc
     1861 aggccgacgc cgtcaacgtg ggccggcccc ggaagctctt cgacttgcag cacaacgcgc
     1921 gcctgcaggt cgactaccgc gacttcgata tgaccaagga cggcacgcgc ttcatcgcca
     1981 cctacgtcgc caaccccgag ttggcgcgcc gccgcatcga cgtgcttctc aacgcgaacg
     2041 aacttctcga gcgcgcggag aagaagggcg ttgcggagta gccccgagcg ccgaccccga
     2101 ttcatgtcga aagggatgcc agaatgacga gttggattgg gaagtcgatc gcgcactacc
     2161 gcgttacgac acaaatcggt gcgggcggca tgggcgaagt gtatcgcgcg agcgattcgc
     2221 gcttgggtcg cgacgtcgcc atcaaggtgc tgcccgaggc gttcgccgcc gaacccgagc
     2281 gaatggcgcg cttcgagcgc gaggcgaagg tgctcgcgtc cttgagccac gccaatatcg
     2341 ccgccatctt cggcttggaa gagtcgaacg gcgcgcgcgc cttggtgatg gagctcgtgg
     2401 acggcacgac cttggccgaa cgcatcgcga agggcgcgct ccccgtcgac gaagcgctcc
     2461 caattgccaa gcagattgcc gaagcactcg agtacgcgca cgaacgttcc atcatccatc
     2521 gcgatctcaa gcccaccaac atcaagctct ccggggacgg cgtcaaagtc ctcgacttcg
     2581 ggctcgcgaa ggcgctttcc gacgacgcca ccgcgtcgaa ttccgatctc tccaagtctc
     2641 ccacgctgac ggccgcatcg accaagctcg gcgtcattct cggaacggcg gcctacatgt
     2701 cgcccgaaca ggcgaaaggg aaagcggtcg accgtcgcgc cgacatctgg gccttcggcg
     2761 cggtgctctt cgagatgttg accggccgtc aggcgttcgc gggcgagacg gtctccgaaa
     2821 cgctcgcatc cgtgatgaag gatcaacccg attgggatgc gctgccggcg tcgacgcccg
     2881 cgcgcgttcg cgacgtgttg cgtcgctgcc tggtaaaaga tccgaaacag cgcctgcgtg
     2941 atatcggcga tgctcgcatt cgcatcgagg aggagttgtc gggcgcgccg gatacagcct
     3001 ccgcgacggc cacgacggcg ggggcgaagc gacgcggcat cccggcggcc ccggtggcga
     3061 tcgtcgcact cgtcgccgcg gcgatcggcg tggccgcagg attcgtcctg cgcaagcccg
     3121 cagtaccccc gatgttccgg gccacgctca cgtttccgga agagatgcgc ctcgacgaag
     3181 agaacgcgtc gattgtcatc tcccccgacg gtcgcacggt agcggtggcc gcgtcggcga
     3241 aggaccatcc gcagcaactt tggcttcgtc gcttcgacag cgacgcgacg acgcccatcg
     3301 ccggcaccaa cggcgccacc tatccgttct ggtcgccgga tggccgtttc gtcgggttct
     3361 tcgccgatcg caagctcaag cgtgtgccga ccaacggagg cacggtgcaa acgatttgtg
     3421 acgccgtcga cgcgcgcggc ggcagctgga gcgagcacgg catgatcatt ttctctcccg
     3481 gcccgctcac cggcctcgaa atcgtcccgg cgtcgggcgg gactccgcaa cctctgacta
     3541 cgctccagaa gaatggagag acgcaccgcc taccccggtt tctcccgggc ggcaagcgcg
     3601 tgctcttcat ggtggggtcc gccgacctgt ctcccgggca cgtcgagtgc ctcgacatcg
     3661 agtccaagga acgcacgccg gtgatgcagt gccggtcgga agcgcgctat ctgggtaccg
     3721 gtcacctcgc gttcatgatc gacgacaacc tcgtggttca gcgtttcgac gcgtcgtcct
     3781 tgaaacttgc gggcgagccg gtaccggtcg cggagtcggt ccaattcaac atttatcggt
     3841 acaccggtgg ctgggacatt tccgagcacg gcgcgttcgc ttacacgacg ggtgtgggag
     3901 tcgagaatgg gcagctcacc tggttcgatc tcgagggacg cgagttggga acgctgggga
     3961 ctcccaatcc catcgtcggg ctgaacatct cgccggatgc gcaacgcgtc gtgacctccg
     4021 agcgcgcgga ccagttcgac ctttggatat gggacgtcgc gaggggcgtg cacacgtgcc
     4081 tgaccttcgg acccgaaccc gcggccagtc ctatttggtc gcgcgacggt cgaacgatct
     4141 acttcggtac cggggccggc gaggttctcg ccaaatccgc cgacggagca tccgccgcca
     4201 cgacggtcta caaacttccc gacacctggc tcgttcccga gagtatggcg ccgaacggga
     4261 aggagatgct cctgtcgaca caattgcgcg agaccgggac cgacatcctc gtgatgacaa
     4321 tggttgactc cacggcccca cgcaagctct tcgcgtcacc cgccaacgag gatgacgcgc
     4381 gggtgtcgcc ggacggacgg tgggttctcg tgacgagcga cgagtcgggg cagccggagg
     4441 tgtacgtcta tcggtatccg tcgctttcgg ggcgttggca agtgtcgtcg gacggagggg
     4501 tcaacggaga atggcttccc aacgggcgag cgattctgta tcgcgcgccc gaccagcggc
     4561 tgatccaggt cgac
//
LOCUS       DASPGK010000078         8564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1307868, whole genome shotgun sequence.
ACCESSION   DASPGK010000078 DASPGK010000000
VERSION     DASPGK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307868"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..156)
                     /locus_tag="VEC56_02095"
     CDS             complement(1..156)
                     /locus_tag="VEC56_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12975.1"
                     /translation="MEELKAYVDQHPEMKKALYVVPKRDDVVGKAANFILHVAERMAK
                     EGRRKAA"
     gene            complement(240..2489)
                     /locus_tag="VEC56_02100"
     CDS             complement(240..2489)
                     /locus_tag="VEC56_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV12976.1"
                     /translation="MAWHDRRNGTDYDIYAQRVDVFGVVQWTTHGVALCSAINDQTNP
                     AIVSDGAGGAIVTWEDYRVGFGDLHAQRVSASGVVQWAMNGVLLVGAAGLQQLPTMIS
                     DGAGGAIATWQDHRNGGVNWDVYAQRINASGVVQWANGVALCVAANDQPFPTIISDGA
                     AGAIVTWSDYRTGATADIYTQRVNASGLVQWTPNGVALCTAADEQSSPTIVSDGTGGA
                     IVTWYDHRTGTNFDIYAQRVNASGVAQWPLNGVAMCTAANQQTSPTLVSDGAGGAIVT
                     WADLRSGTNNDIYAQRVNASGVAQWPLNGVALCTAADVQVYPVIAPAGAGGAIVAWQD
                     SRNGNSDAYSHRVDASGVVQWTTGGVAVCTAADYQADLAIVSDGAGGAIVTWADLRSG
                     NFDIYAQRVEVDFGTWGHPEPTVISVADVPNDQGGKVAVNWKRSGHDALPLQEISHYS
                     VWRAIDLVALSSAEAEGWTDPATIGASFNGRAAFHERVGTTDNYWEWVANQSATHDLG
                     YAFAAATRADSVAANNAQHYFRVLAHTSNPYVLYKSNAMSGHSVDNLAPAAPLFLTAQ
                     RVGPDVHLEWNRVRVADLKNYAVYRATSMGVTPIPPNLLANDTDTVLVDASAPTSALY
                     YIVTAYDVHENQSAASNEANVGATTGVGETPAITALALRPNHPNPFAGTTELEIGLPQ
                     ESDVSIQIYDVLGRVVRDARVPRMSAGWQRVALSDRGVDGKPLASGVYFCRVTAAGRT
                     VTQKMVIAR"
     gene            complement(2919..4643)
                     /locus_tag="VEC56_02105"
     CDS             complement(2919..4643)
                     /locus_tag="VEC56_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="activator of (R)-2-hydroxyglutaryl-CoA
                     dehydratase"
                     /protein_id="HXV12977.1"
                     /translation="MSVDLNVAQPAAESLRHRAPQAPRAKKPITHFKRPVERPFTRAD
                     RPHTTVLFGGLTWSHERLIQGAWEGLGYKCEVVPVPDVAAFQLGKEYGNNGQCNPTYF
                     TVGNLVQFLQKLESQGMPHEKIVNDYVFITAGACGPCRFGMYEAEYRLALRNSGFEDF
                     RVILFQQSKGLSQEDVEAGLEMNLEFFLAMLNSMNIGDMLNEIGYLVRPYEVNEGATN
                     AVLANAQEYLAGVLKNQPKAKIGDGVEKILRRTGLYNQADYAVKFGRQLTSSYYTDAL
                     HTVRDQFADIEVDRFRVKPIVKITGEFWAQTTEGDGNYNMFRFLEKEGAEVIVEPIGT
                     WIMYMIHQFKQKLRDRKGLEDKTPMPSWKKEPHRRAAVEARYRKRMARMTVAERIFQR
                     EYTKLQHALGGGILHELVCQYELQRMGHPYYNSRAGGGEGHLEVAKNIYYSSKGIAHM
                     VVSLKPFGCMPSTQSDGAQAAVTEHYKDMIYLPIETSGEGEINAHSRVQMALGNARIK
                     ARQEFADALAKTGRTIEELRAYVDGHPEMKKALYLVPKRDDVVGKAANFVLHVAERMA
                     KEGSRRVA"
     gene            complement(4737..5282)
                     /locus_tag="VEC56_02110"
     CDS             complement(4737..5282)
                     /locus_tag="VEC56_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV12978.1"
                     /translation="MRVPPKRIEPVRFTLSTCTLREWRRGDEPSLVRHANNRKVWINL
                     RDSFPFPYTPADAKGWIRLATTSGLNEVFAIDVDGFAVGAIGIKPGEDVHRLSAEIGY
                     WLGEEFWNRGIATEAVKAVTEHAFETLGMKRVHAAVFEWNTASMRVLEKAGFVKEGVL
                     AKSALKDRKVIDQVVYARVRE"
     gene            5361..6617
                     /locus_tag="VEC56_02115"
     CDS             5361..6617
                     /locus_tag="VEC56_02115"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HXV12979.1"
                     /translation="MNRRAPFAPLCIALAGIAVPAAAGNWTGKETPKDGVVHVTNPGA
                     PSDGKSTVTLQELWRAGGDDEEVIFGVVNDVAVDTQGNLYALDTQLSQVSVFDPDGNL
                     LRTIGREGEGPGEFRRASQLFVTPDNKIAVAQMMPGKLVLLTPDGKPGGDFPMPAAPD
                     GGTQMIWDAGAAGRSVVIGGGTFAQKDNKMTRTTALQLYGMDGTSRGTLVERTSTDDI
                     ANMKFDEKSARRPVWTASADGKIYLNDNFDVYEIKYYGTDGKLARVATRDYQHRARSK
                     AEMEEDRPRIMMRRGGGGGRRFDAEPSPTDPDVIQMFARDDGTLWVLSSRGRDCPKGT
                     IGRFDVFDANGHFVREVTVQGEGNAKADGVTIAGDRLILLKGLRSAQRAMMAAMSDDS
                     AAEEEEEGEPMAIVSYRLDPQPTAKR"
     gene            6833..7975
                     /locus_tag="VEC56_02120"
     CDS             6833..7975
                     /locus_tag="VEC56_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12980.1"
                     /translation="MKQLRRSTLLLLLALSLAAMFWACSEDPGNNVASPEPENDLLAA
                     VQAHQEEFEQAIAIHNRAVPGILSTKGIVGTAIGADDNGLPVVKIYTSGPIAKGPLPQ
                     DVGGLPIVIEETGPIKAYKGGGGGGSSGQAKQTPPVKMGTSGGWRFDLANGFCCGGTL
                     GSLVQKGGTKYVLSNYHVLYADIVNGGNSRTAQAGDPVIQPGLIDVACNANTAQNIAS
                     LVANGGTLPDPGNPDAVDAAIASVTSGMVDETGSILNVGTISATTRSAAVGLAVKKMG
                     RTTGLTRSTISGINGAFSIQYENECAGGASFVQSYSGQIVISNRRCGFQNGGDSGSLL
                     VEDVTTNPRAVGLCFAGSVTCNNSAIAIANPIGDVLSKLGATMVGQ"
     gene            8054..8269
                     /locus_tag="VEC56_02125"
     CDS             8054..8269
                     /locus_tag="VEC56_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12981.1"
                     /translation="MKPIDQVIAERAGEWLKIDGVVGVYQGVAPDGAEIICIAVRKET
                     PALAARISSQAGGHRVEIVATGPIEPF"
     gene            complement(8277..>8564)
                     /locus_tag="VEC56_02130"
     CDS             complement(8277..>8564)
                     /locus_tag="VEC56_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12982.1"
                     /translation="EPPNTLGNVVLLASDRAMEMEEDALGDPHAALPFPYHHSRVVER
                     RHAWANRFEPNPAGVPVLTDDKNPVDLWAEGINWAARRHMHREQAWDRFAW"
BASE COUNT         1470 a         2812 c         2757 g         1525 t
ORIGIN      
        1 ttaggcagcc ttccgccgcc cctctttcgc catccgctcc gcgacgtgga ggatgaagtt
       61 ggccgccttg ccgacgacgt cgtcacgctt ggggacgacg tagagcgctt tcttcatctc
      121 ggggtgctgg tcgacgtagg ctttcagctc ttccaacgaa cgccccgtct tggcgagaca
      181 atccgcgaac tcctggcgcg ccttgatgcg cgcgttgccg agccagcgag attcctcagc
      241 taccgagcaa tcaccatctt ctgggttacc gtgcggccgg ccgccgtgac ccggcagaag
      301 tacacgccgc tcgcgagcgg cttgccgtcg acaccgcggt cgctcaacgc cacgcgctgc
      361 cagcccgcgc tcatgcgcgg cacgcgcgca tcgcgaacca cacggccgag gacgtcgtag
      421 atctggatcg atacgtcgct ctcttgcggc aggccgatct ccaattctgt cgtgcccgcg
      481 aacgggttgg gatggttcgg gcgtagcgcg agtgcggtga tcgccggcgt ttctccgacg
      541 cccgtggtcg cacctacgtt ggcctcgttc gacgcggcgc tctggttctc gtgcacgtcg
      601 taagccgtga cgatgtagta gagcgcgctc gtcggcgcac tcgcgtccac cagcaccgtg
      661 tccgtgtcgt tcgcgaggag gtttggcggg atcggtgtca cacccatgga tgtcgcccgg
      721 tagaccgcgt agttcttgag atccgcgacc cgcacgcgat tccactcgag atgcacgtcc
      781 ggcccgacgc gctgcgcggt caagaacagc ggtgccgccg gagcgagatt gtcgacagaa
      841 tggccactca tcgcattgga cttgtaaaga acgtacgggt tgctcgtatg cgccagcacg
      901 cgaaaatagt gctgcgcgtt gtttgcggcg accgagtccg cacgcgtcgc tgcagcgaat
      961 gcgtatccga ggtcgtgtgt ggccgattgg ttggccaccc attcccagta gttgtcggtg
     1021 gtgccgacgc gctcgtggaa tgccgcccgg ccattaaagc tcgcgccgat cgtcgccggg
     1081 tccgtccatc cctccgcctc tgcgctggag agagcgacga gatcgatggc acgccagacc
     1141 gagtagtgac tgatctcttg cagaggaaga gcgtcgtgcc cgctgcgctt ccagttgacg
     1201 gcgactttgc cgccctggtc gttgggaacg tcggcaaccg aaatcaccgt aggctccgga
     1261 tgcccccacg tgccgaagtc gacctctacc cgctgcgcgt agatatcgaa atttccgctt
     1321 cgaaggtcgg cccacgtcac gatcgctccc ccggcaccgt ccgagacgat cgcgaggtca
     1381 gcctgatagt ccgcggccgt gcacaccgcg accccgcccg tcgtccactg caccacgccg
     1441 gaagcgtcca cccgatgcga ataggcgtcg gagttgccgt ttcgagagtc ctgccacgcc
     1501 acgatcgccc ccccggctcc agccggggcg atcacgggat atacctgtac atccgccgca
     1561 gtgcacaagg cgaccccgtt caatggccac tgcgcgaccc ccgaggcgtt cacccgctgc
     1621 gcgtagatgt cattgttcgt accgcttcga aggtcggccc acgtcacgat cgcaccccct
     1681 gcaccgtccg agacgagcgt gggagacgtc tgctggttcg cggcggtgca catggcgact
     1741 ccgttcaatg gccactgcgc gacccccgag gcgttcaccc gctgcgcata gatgtcgaag
     1801 ttggtgccgg ttcgatggtc gtaccacgtc acgattgctc cccccgtacc gtcggagacg
     1861 atcgtcgggg aggactgctc gtcggcggcg gtacacagtg caaccccgtt cggcgtccac
     1921 tgcaccagcc ccgaggcgtt cacgcgctgg gtgtagatgt cggcagtggc accggttcga
     1981 taatcgctcc acgtcacgat tgcccccgcg gccccatccg aaatgatcgt ggggaagggc
     2041 tggtcgttgg cggcgacgca aagagccacc ccgttcgccc actgcacaac tcccgaagca
     2101 ttgatccgct gcgcgtagac atcccaattg acgccaccgt ttcgatggtc ctgccacgtc
     2161 gcgatcgccc ccccggcccc gtccgagatc atcgtgggta gttgctgtaa gcccgcggcg
     2221 ccaacaagaa gcacaccgtt catcgcccac tgcaccactc ccgacgcgct gactcgctgg
     2281 gcgtggaggt ccccgaagcc gactcgatag tcctcccacg tcacgatcgc ccccccggcg
     2341 ccgtccgaga cgatagcggg gttggtctga tcgtttatag cactacagag agccaccccg
     2401 tgggtcgtcc actgcaccac cccgaagacg tccacccgct gcgcatagat gtcgtagtcc
     2461 gttccgttgc gacggtcgtg ccaagccacg atcgcccccc cggctccatc cgatgcgata
     2521 gcggggaaat cttgaccgtt cgcagcggta gtgataggca ccccgtcgag ttgccattgc
     2581 gcttggatga cgcgcggatg aaggaccacc gatagaacga ggagcgaaac cgcgagggtt
     2641 ttcatgtgcg gcctccggcg aggagacgcg gcgtcggcga ccgctgaagt tgggagagaa
     2701 gacacccggc cgacgggttc gggtgtcgac gactgggcgg agctcgcgat actctggggg
     2761 ctctcgcgtt ggcaggagta tatcaccggc ccgggcgctt ttcagacgcg agatccaccc
     2821 gtccacgcga ggcggcaggc gagataccgg cgcatacccc aacgaaaggc ccggcaatcc
     2881 gccgggcctt tctccaaatc tttgcagacg gatggagcct acgccacccg tcggctcccc
     2941 tctttcgcca tccgctcggc cacgtgcagc acgaagttgg ccgccttgcc gacgacgtcg
     3001 tcgcgtttgg gaacgaggta gagcgccttc ttcatctcgg ggtgcccgtc gacgtacgca
     3061 cgcagctctt cgatcgtacg gcccgtcttg gccagggcat ccgcgaactc ctggcgtgcc
     3121 ttgatgcgcg cgttgccgag cgccatctgc acgcgcgagt gcgcgttgat ctcgccttcg
     3181 ccggaggtct cgatgggcag gtagatcatg tccttgtagt gctcggtcac ggccgcttgc
     3241 gcgccgtcgg attgcgtgct cggcatgcag ccgaagggct tcaatgaaac caccatgtgc
     3301 gcgatgccct tgctcgagta gtagatgttc ttcgcgactt cgaggtgacc ctcgccgccg
     3361 ccggcgcgcg agttgtagta cgggtgcccc atgcgctgga gctcgtactg gcataccagc
     3421 tcgtggagaa tcccgccgcc cagcgcgtgc tgaagcttgg tgtactcgcg ctggaagatc
     3481 ctctcggcca ccgtcatgcg cgccatccgc ttgcggtaac gggcttccac ggcggcgcga
     3541 cggtgcggct ccttcttcca cgacggcatc ggcgtcttgt cctcgaggcc cttacggtcg
     3601 cggagcttct gtttgaattg atggatcatg tacatgatcc aggtcccgat gggctcgacg
     3661 atcacctcgg cgccttcctt ctcgaggaag cggaacatgt tgtagttgcc gtcgccctcg
     3721 gtggtctggg cccagaattc gcccgtgatc ttgacgatgg gcttgacccg gaagcgatcg
     3781 acctcgatgt cggcgaattg gtcgcgcacg gtatgcaagg catccgtgta atacgagctc
     3841 gtgagctggc gcccgaactt caccgcgtag tcggcctggt tgtacaggcc ggtgcggcgc
     3901 aggatcttct cgacgccgtc gccgatcttg gccttgggct ggttcttcaa gacgccggcg
     3961 aggtactctt gggcattcgc cagcaccgcg ttggtggcgc cctcgttcac ctcgtaaggg
     4021 cgcacgaggt aaccgatctc gttgagcatg tcgccgatgt tcatcgagtt gagcattgcc
     4081 aggaagaact cgaggttcat ctcgaggccg gcttcgacgt cctcttgcga gaggcccttc
     4141 gactgctgga acaagatcac gcgaaaatct tcgaagccgg agttgcgaag cgccaggcga
     4201 tactcggcct catacatgcc gaagcggcac ggcccgcagg cacccgcagt tataaaaacg
     4261 taatcgttga cgatcttctc gtgcggcatg ccttggcttt cgagcttttg caggaactgc
     4321 acgagattgc cgaccgtaaa gtaagtcgga ttgcactggc cgttgttgcc gtactccttg
     4381 ccgagctgaa acgcggcgac gtcgggcacc ggcacgactt cgcacttgta gcccaaaccc
     4441 tcccacgcgc cctggatcaa tcgctcgtgg ctccacgtga gcccgccgaa gaggacagtg
     4501 gtgtgcggtc ggtcggcgcg cgtgaacggg cgctcgacgg gtcgcttgaa atgggtaatg
     4561 ggtttcttgg cgcgcggcgc ctgcggcgcg cggtggcgga gcgactcggc ggcaggctgg
     4621 gcgacgttga gatcgacgga catggcggcc tcctcatata cgacggaacg tcaagacgcc
     4681 gccagtatcc cccgagcctg tgggcgcgcg caacaagata gtggaggcgt tgtattctat
     4741 tctcggaccc gggcgtagac gacctggtcg atgaccttgc ggtccttgag cgcgctcttg
     4801 gccagcacgc cctctttcac gaagcccgcc ttctcgagca cccgcatcga cgcggtgttc
     4861 cattcgaaga cggcggcgtg cacgcgtttc atccccaggg tctcgaacgc gtgttcggtc
     4921 acggccttca cggcttcggt cgcgatgccg cggttccaga attcttcgcc cagccaatag
     4981 ccgatctcgg cgctcaagcg atgcacatcc tccccgggct tgatcccgat cgcgcccacc
     5041 gcgaagccgt ccacgtcgat cgcgaacacc tcgttgagtc cgctggtcgt ggccagtcga
     5101 atccacccct tcgcgtcggc cggcgtgtag ggaaagggga aggaatcgcg gaggttgatc
     5161 cacaccttgc ggttgttggc gtggcgaacc agggagggct cgtcgccgcg ccgccattcg
     5221 cgcagggtgc aggtcgacag cgtgaagcgg accggctcga tgcgtttcgg ggggactctc
     5281 atatgggaac cgccgcgcgt accagcgtgt tcaatcgccg tgcagtatac ttcaccgcac
     5341 acctttcgag gaggagatcg atgaatcgac gcgccccgtt cgccccgctt tgcatcgccc
     5401 tcgcgggcat cgccgtcccg gccgcggccg gcaactggac cggcaaggaa acccccaagg
     5461 atggcgtggt tcacgtgacc aaccccgggg ccccctcgga cggcaagtcg acggtgacgt
     5521 tgcaggagtt gtggcgcgcc ggcggcgacg acgaagaggt catctttggc gtcgtcaatg
     5581 acgtggcagt cgacacccag ggcaacctct acgccctcga cacccaactg agtcaagtca
     5641 gcgtcttcga ccccgacggc aatctcctgc ggacgatcgg tcgcgaaggc gaaggccccg
     5701 gcgaattccg gcgcgcgtcg cagctgttcg tgacacccga caacaagatc gcggtggcgc
     5761 agatgatgcc gggcaagctc gtgctgctca ccccagacgg caagcccggc ggcgacttcc
     5821 ccatgcccgc cgctcccgac ggcggtacgc agatgatctg ggacgcgggt gctgccgggc
     5881 gctcggtcgt catcggagga ggtaccttcg cacagaagga taacaagatg acgcgcacga
     5941 cggcgctgca gctgtacgga atggatggca cctcgcgcgg cacgctggtc gagcgcacga
     6001 gcaccgacga catagcgaac atgaagttcg acgagaagtc ggcgcgacgc ccggtctgga
     6061 ccgcgagcgc cgacggcaag atctatttga acgacaactt cgatgtctat gagatcaagt
     6121 actacggcac cgacggcaag ctcgcgcgcg tcgcgacgcg cgactaccag caccgcgcgc
     6181 gctcgaaggc cgagatggag gaagaccggc cgcgcatcat gatgcgccgc ggcggaggcg
     6241 gcggccggcg cttcgacgcc gagcccagtc ccaccgatcc cgacgtgatc cagatgttcg
     6301 cgcgcgacga cggcacgctg tgggtgttgt cgagccgcgg ccgcgattgc cccaagggca
     6361 cgatcgggcg cttcgacgtc ttcgacgcca acggacactt cgtgcgcgag gtcactgtgc
     6421 agggcgaagg aaacgccaag gccgacgggg tgaccatcgc gggcgaccgg ctcattctgc
     6481 tcaagggcct gcgctcggcc cagcgcgcca tgatggcggc gatgtcggac gattcggcgg
     6541 cggaggaaga ggaagagggc gagccgatgg cgatcgtgag ctaccgcctc gatccgcagc
     6601 cgacggccaa gcggtagtaa ctcccgaagt tgcgcgcctg cgggccccgg cggatgttcc
     6661 tccgtcgggg cccgtggcgt tcttgaagca agccgtgaaa cttttctcaa agccccgttg
     6721 gggctgcatc cccgttgact cgcttcggaa ccccgagtag cctccgtaca ttcgtttccg
     6781 tggggagaat tctcctcgct cgacatcctc accagcgtct cggaggtttc ggatgaaaca
     6841 actgcggcgt agtacgttac tcctgctgct cgccttgtcg ctcgccgcga tgttctgggc
     6901 gtgcagcgaa gatcccggca acaacgttgc gtcccctgaa cccgaaaacg acctgctggc
     6961 cgcagtccag gcccaccagg aagaattcga acaggcgatc gcaattcaca accgtgccgt
     7021 tcccggaatc ttgtcgacca agggcatcgt cggtaccgcc atcggcgcgg acgacaacgg
     7081 cctgccggtc gtcaagatct acacgtccgg accgatcgcg aagggtccgc tcccgcaaga
     7141 cgtcgggggc ctgccgatcg tgatcgagga aacagggccg atcaaggcgt acaagggcgg
     7201 cggtggcggt ggcagcagcg gtcaagccaa gcagacgccg cccgtgaaga tggggacgtc
     7261 gggcggctgg cgcttcgacc tcgccaatgg attctgctgc ggcggcacgc tgggctcgct
     7321 ggtccagaag ggcggcacca agtatgtgct ctccaactac cacgtgctct atgcggacat
     7381 cgtcaacggc ggcaacagtc gtaccgccca ggcgggcgac cctgtgatcc agccgggcct
     7441 gatcgacgtc gcgtgcaacg cgaacacggc gcaaaacatc gcgtcgttgg tcgccaatgg
     7501 cgggacgtta ccggatcccg gcaacccgga cgccgtcgac gcggccatcg cgtcggtgac
     7561 ctcgggcatg gtggacgaaa ccgggtccat cctgaacgtc ggcacgatct cggcgacgac
     7621 gcgcagcgcg gcggtcggcc tcgcggtcaa gaagatgggt cgcaccaccg gcttgacgcg
     7681 cagcacgatc agcggcatca acggcgcgtt ctcgattcag tacgagaacg agtgcgcggg
     7741 cggagcgtcc ttcgtccaga gctactcggg ccagatcgtg atctcgaacc gccgctgcgg
     7801 cttccagaac ggcggcgact cgggctcgct cctggtcgaa gacgtgacga ccaatccgcg
     7861 cgcggtgggg ctatgcttcg ccggtagcgt gacgtgcaac aactcggcga tcgcgatcgc
     7921 gaatccgatc ggcgacgtgt tgagcaagct gggcgcgacg atggtgggac agtagaccaa
     7981 ccgacgtcat tcgccagcgc gagcacccgt ctcggtcctc gaggcgggtg ctcgtgtttt
     8041 taggaggctc gatatgaaac cgatcgacca agtgatcgcg gaacgcgcgg gcgagtggct
     8101 gaagatcgac ggcgtggtcg gcgtctatca gggcgtggcg ccggacggcg cggagatcat
     8161 ctgcatcgcc gtgaggaagg agacgccggc gctggccgcc cgcatttcct cccaggccgg
     8221 cggccaccgc gtggagatcg tcgcgaccgg gcccatcgag cccttctagc cgcccatcac
     8281 cacgcgaagc ggtcccaggc ttgttcgcgg tgcatgtggc gtcgcgccgc ccagttgatg
     8341 ccctccgccc ataggtcgac cggattcttg tcgtcggtga gaaccgggac acccgccggg
     8401 ttcggctcga agcgattcgc ccacgcgtgt cgccgctcga ccacgcgcga atggtggtac
     8461 gggaacggga gcgcggcgtg cgggtcgccc agcgcgtcct cttccatctc catcgctcgg
     8521 tcggaagcga gcaagacgac gttgccgagc gtgttcggcg gttc
//
LOCUS       DASPGK010000079         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1191133, whole genome shotgun sequence.
ACCESSION   DASPGK010000079 DASPGK010000000
VERSION     DASPGK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191133"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VEC56_02135"
     CDS             <1..354
                     /locus_tag="VEC56_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HXV12983.1"
                     /translation="GSTVHAAGAPKGYRALLDPLPASVRFVTSFDQTTDVVHLFTTKK
                     ADLAKALARFRKKLAPDAVVWVSWPKKSAQVPTDITEDVVRDVALPLGFVDVKVCAID
                     DVWSGLKLVVRKALR"
     gene            351..1535
                     /locus_tag="VEC56_02140"
     CDS             351..1535
                     /locus_tag="VEC56_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HXV12984.1"
                     /translation="MTHSPGAPASRVASVDLIRGAVMVLMAIDHVRVYAGVPAGGPEP
                     AVFFTRWITHFCAPAFVFLAGSSAFLYGRTHAGLSRFLTTRGLWLVFLELTFMRWAWT
                     FNFDYAHYEMAGVIWVIGWSMILMAALVKLPAKVVGAVGVVIIAGHNLMDPLIGERID
                     AVAGSSLAWLWKILYLGFWAGPIQFGPDGPTLIVLYSIIPWVGVMAAGYGFGRVLILE
                     PARRDRVCMTLGLGAIALFIVLRGLNWHGDPFPWNASEGMPAPLAFLNTTKYPASLSF
                     LLMTLGPTIALIPLLERARGTLARWITVFGRVPFFFYVLHIPLIHALALVVSQIRLGE
                     VTPWLFANHPMGVPPAPEGYMWSLALLYAVWAVVIAMLYFPCRWFAGLKARRKDPWLS
                     YL"
     gene            1594..>3413
                     /locus_tag="VEC56_02145"
     CDS             1594..>3413
                     /locus_tag="VEC56_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXV12985.1"
                     /translation="MQAPSPAKNARSLALVVAALGVVYGDIGTSPLYAIRECFHGPHA
                     VAVTPEHVLGVLSLVFWSLVVIISIKYLMVVMRANNQGEGGIIALMALVADLDGRPSK
                     RQWMIITMGLFGAALLYGDGMITPAISVLSAIEGLEVVTPAFTPYVVPITALILVLLF
                     RIQKNGTGRIGRFFGPLTFAWMLALAMWGILSIVQSPVVFKAVNPAYGVRFIAEYGIE
                     SVKTLGVVFLVVTGGEALYADMGHFGLGPIRRAWFSIVLPALALNYFGQGAFLLRSPE
                     HAANPFYYMLPEQALIPMVILATTATTIASQAVIAGAFSLTWQAVQLGFIPRVQVRHT
                     SHEEIGQVYIPFVNTGLLLATLALVFGFRTSSNMAAAYGLAVSTDMVITTVLLFIAMR
                     RLWKWNLFLAFSVCALFLVVDLAFFSANVIKIADGGWFPLVVGIGILIVMTTWRTGRR
                     LLTQRMQERRRSFEDLKKTRAEVTRVPGTGVFLERDASGFPATLRRIQQHLNVMFDRV
                     IILSIRTERVPRIAASERVSVESMGDNVYRVIARYGFNEMPHVPKLLRRCRENGLHVD
                     PSEVTYVMSRETLIATRRPGMAMWRERLFAFMSRNAQRATA"
BASE COUNT          503 a         1111 c         1091 g          708 t
ORIGIN      
        1 ggctccaccg tccacgccgc cggcgcgccg aagggctatc gcgcgctcct cgacccgctc
       61 cccgcatccg tccgtttcgt cacctcgttc gaccagacca ccgacgtcgt ccatctcttc
      121 acgaccaaga aggccgatct cgccaaggcg ctcgcgcgat ttcgcaagaa actcgcgccg
      181 gacgcggtgg tgtgggtatc gtggccgaag aagtctgcgc aggtgcccac cgacatcacc
      241 gaggatgtgg tgcgcgatgt cgcgctcccg ctgggattcg tcgacgtcaa ggtgtgcgcg
      301 atcgacgacg tgtggtcggg actcaagctg gttgtccgga aggcgctgcg atgacccatt
      361 ctcccggtgc gcctgcttcg cgcgtcgcct ccgtcgatct gattcgcggc gcggtgatgg
      421 tccttatggc catcgaccac gtgcgcgtct acgccggggt tcccgcgggc ggacccgagc
      481 ccgcggtctt cttcacgcgc tggatcacgc acttctgcgc gcccgcgttc gtcttcctgg
      541 ccgggagcag cgcgttcttg tatggtcgca cccacgccgg actctcgcgc ttcctcacga
      601 cgcgcggcct gtggctcgtg ttcctagagc tcaccttcat gcgctgggcg tggacgttca
      661 atttcgatta cgcgcactac gagatggcag gcgtgatctg ggtcatcggg tggtcgatga
      721 tcctcatggc cgcgctggtg aaactccccg cgaaagtggt cggcgccgtc ggcgtcgtca
      781 tcatcgcggg acacaatctc atggacccgc tgatcggcga gcgcatcgac gcggtcgccg
      841 ggagcagcct cgcgtggctc tggaagatcc tctatctcgg gttctgggcc ggcccgattc
      901 agttcggacc cgacgggccc accttgatcg tgctctattc gatcattcca tgggtcggtg
      961 tcatggcggc gggctatggg ttcggtcgcg tgctcatcct cgagcccgcg cgtcgcgacc
     1021 gcgtttgcat gacgctggga ctcggcgcga tcgcgctgtt catcgttctg cgcggactca
     1081 attggcacgg cgatccgttc ccgtggaatg catcggaagg aatgccggcg ccgctcgctt
     1141 tcttgaacac gaccaagtat ccggcgtcgc tctcgttcct cttgatgacg ctgggcccga
     1201 ccatcgcgct cattccgctc ttggagcgcg cgcggggaac gttggcgcgc tggatcaccg
     1261 tgttcgggcg cgtgccgttc ttcttctacg tgctccacat tccgttgatc cacgcgctag
     1321 cactcgtggt ctcccagatt cgactgggcg aggtgactcc gtggctgttt gcgaatcacc
     1381 cgatgggtgt gccgcccgcg ccggaaggtt acatgtggag tctcgcgctg ctgtatgcgg
     1441 tgtgggcggt ggtgatcgcg atgctctatt tcccgtgccg gtggttcgcg ggcctcaagg
     1501 cgaggcggaa ggacccgtgg ctgagttacc tgtgacaccg gccacggata aacgatagct
     1561 gtcctggcgc gcggcgtgta tagtcgccgg tcgatgcagg ccccgtcccc cgcaaagaac
     1621 gcccggtcgc tcgcgctcgt ggtggccgct ctcggtgtgg tgtacggcga catcgggacc
     1681 agtccgctct acgccatccg cgagtgcttc catggcccgc acgcggtggc ggtcacgccg
     1741 gagcacgtcc tcggcgtcct ctccctcgtc ttctggtcgc tcgtcgtcat catttcgatc
     1801 aagtatctaa tggtcgtcat gcgcgccaac aaccagggcg aaggcggcat catcgcgctc
     1861 atggccttgg tcgccgacct cgacggacgc ccgtcgaagc gccagtggat gatcatcacg
     1921 atgggactgt tcggcgccgc actcctctat ggggacggga tgatcacgcc cgccatttcc
     1981 gtattgagcg cgatcgaagg cttggaagtc gtgaccccgg cgttcacgcc atacgtggtg
     2041 ccgatcacgg ccttgattct cgtcctgctg tttcgcatcc agaaaaacgg aaccggccgc
     2101 atcgggagat tcttcggacc cctcacgttc gcgtggatgc tggcactggc gatgtggggc
     2161 atcctctcga tcgtccagtc gccggtcgtc ttcaaggccg tcaatccggc ctacggtgtg
     2221 cgcttcatcg cggagtacgg aatcgagtcg gtgaagacgc tgggcgtggt gttcctggtc
     2281 gtcaccggcg gcgaggcgct gtacgccgac atgggccact tcggtctcgg ccccatccgg
     2341 cgcgcgtggt tctcgatcgt gttgcccgca ctcgcgctca attacttcgg acaaggggcg
     2401 tttctcttgc gctcgcccga gcacgccgcc aatccgttct attacatgtt gccggagcag
     2461 gctctcattc cgatggtgat cctcgccacc accgcgacca cgatcgcgtc gcaggcggtg
     2521 atcgcggggg cattctcgct gacgtggcag gcggtgcagc tgggcttcat accgcgcgtg
     2581 caggtgcgac acacgtcaca cgaggaaatc gggcaggtct atattccgtt cgtcaacaca
     2641 ggcctcttgc tggccacact cgccctcgtg ttcgggtttc ggacctccag caacatggcc
     2701 gccgcgtacg gtcttgcggt cagcaccgac atggtcatta ctaccgttct gcttttcatc
     2761 gccatgcgcc ggctgtggaa atggaacctg ttcctcgcgt tttcggtgtg cgcgttgttc
     2821 ctcgtggtcg acttggcgtt cttcagcgcg aacgtgatca agatcgccga cggcgggtgg
     2881 tttccgctgg tggtgggcat cggtatcctg atcgtcatga ccacgtggag gacggggcgc
     2941 cgcttgctga cgcagcgaat gcaggagcga cgccgctcgt tcgaggatct caagaagacg
     3001 cgcgccgagg tcacccgcgt gcccggtacc ggggtcttcc tcgagcgcga cgccagcggc
     3061 tttcccgcca ccttacggcg catccagcag cacttgaacg tgatgttcga ccgcgtgatc
     3121 atcctctcga ttcgaaccga gcgcgtgccg cgcatagcag cgagcgaacg cgtgagcgtg
     3181 gagtcgatgg gggacaacgt ctaccgcgtg atcgcgcgct acggcttcaa cgaaatgccg
     3241 cacgtgccca aactgctgcg ccggtgccga gagaacgggt tgcacgtcga ccccagcgag
     3301 gtgacctacg tgatgagccg cgagacgctg atcgccaccc ggcgcccagg catggcgatg
     3361 tggcgcgagc ggctgttcgc gttcatgtcg cgcaacgcgc agcgcgccac ggc
//
LOCUS       DASPGK010000080        16192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1074241, whole genome shotgun sequence.
ACCESSION   DASPGK010000080 DASPGK010000000
VERSION     DASPGK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 16192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16192
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074241"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /gene="pgk"
                     /locus_tag="VEC56_02150"
     CDS             <1..488
                     /gene="pgk"
                     /locus_tag="VEC56_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXV12986.1"
                     /translation="KYLAMCKELLAKTRPGRGRLLLPVDTIVAQEIDDNAPAREVSIA
                     DIPEDWIGVDVGPKTVDLFKAQIAGAKTVFWNGPMGVFEKPSFAEGTKAVARAIADAT
                     DRGAKTIVGGGDSVAAINLMGIANRFTHISTGGGASLEFMEGRTLPGIEALCDVAEVA
                     G"
     gene            514..1281
                     /gene="tpiA"
                     /locus_tag="VEC56_02155"
     CDS             514..1281
                     /gene="tpiA"
                     /locus_tag="VEC56_02155"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXV12987.1"
                     /translation="MPRPKLIAANWKMNKTISEAVAFASELRAALPSVPGHCDLVVIP
                     TFFGVRPVVEALAGARVWVGAQDLHWEKSGAFTGEVAADMVKDAGAMYVLVGHSERRH
                     GLGETDAVVAKKLAAAQGAGLTPILCVGETLSERESGLQAHVVERQLAVAFTGVSPDD
                     ARATVIAYEPVWAIGTGRTASPDDAVSMHRTIRGWVQGRYGPVVAKAIRIQYGGSVNP
                     TNAADLLSRDEIDGLLVGGASLEAASFLGIARAAGPR"
     gene            1399..1773
                     /gene="secG"
                     /locus_tag="VEC56_02160"
     CDS             1399..1773
                     /gene="secG"
                     /locus_tag="VEC56_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXV12988.1"
                     /translation="MLFNILIGLHVIVCVVLVLVVLLQSSKGGGLAGAFGGGGGQTMF
                     GGQETATFLSKATTYLAVTFMILSLLLAFLSARRSAPGSRSLVREAAEQQQNVIPSGQ
                     SIDEILGGADTTAAGADTTSPR"
     gene            1790..1876
                     /locus_tag="VEC56_02165"
     tRNA            1790..1876
                     /locus_tag="VEC56_02165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1824..1826,aa:Leu,seq:gag)
     gene            1997..3421
                     /locus_tag="VEC56_02170"
     CDS             1997..3421
                     /locus_tag="VEC56_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HXV12989.1"
                     /translation="MTYAKLLGIAALIGGVGTGASAPAADLKSTPADQKSVSITIYND
                     NLGLVKDVRTLDLKTGTQSLWFQGVAAHIDPTTVHIRSIDAPNALSVIEQNFEYDLVS
                     PERLMEKYIGQTVELVTSRENKDDETIRAKLIGIQGGTVYQLENGKIAVNPPGRVILP
                     ALPAGLISEPSLVWLLDTSKTKHTVEASYLTGGIGWRSDYVAVLSADDSKADLSGWVT
                     IDNQSGATYENASLKLVAGDVHRARPEYAAVPQMADMAVRSLAKEEQFREEAFFEYHL
                     YTLERPATVKDRQTKQISLLSAEGAKVKKTFVVQPQYAYWFNRAADVEKPKVGVYVSL
                     DNSKKNGMGMPLPKGIVRVYKKDSKSELQFIGEDRIDHTPEDETIRIKMGDAFDVVAE
                     RVQTNFEVLSSGHLYRSSYKVTVRNHKAEDITVQVVELLSGDWAITTSSYPHVKEASH
                     RARFDVPVKSKGSAELTYTAEIRY"
     gene            3545..4033
                     /locus_tag="VEC56_02175"
     CDS             3545..4033
                     /locus_tag="VEC56_02175"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HXV12990.1"
                     /translation="MDQTSGLDGNALVLAVGALGVVGIVIGVLALVRLRAISGRFSWV
                     TGKDAGGVDTLPALLAAVEGSQRDIQNLQTEFKLASIESRTHFKRLSIVRYNAFEGVA
                     GQQSYSLCLLDENQNGILISNLVGTNFSRSYAVEVKTGEPARPLGEEEKQALASAIKN
                     GN"
     gene            complement(4036..4770)
                     /locus_tag="VEC56_02180"
     CDS             complement(4036..4770)
                     /locus_tag="VEC56_02180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV12991.1"
                     /translation="MVATVAMFFVQNLFPSVEGILALTPRDVVEKLYLWQLVTYMFLH
                     GDFWHLFFNLIVLFFLGNMVEGAWGRKRFLRYYVVCGVGGALLHMMFDYNASVVGASG
                     AIFGLYLAAAMLFPDAYVYLYFLVPIKIKYLVIGLAVLQLANGIAGKAGIAYFAHIGG
                     MVAGLTFFRDEVARRLGWTGGARRRGPTWRPRVGFGTRRGPRASADNIDSILDKISAK
                     GYENLSPTEKRILENYSKQQREEPEE"
     gene            5142..5477
                     /gene="trxA"
                     /locus_tag="VEC56_02185"
     CDS             5142..5477
                     /gene="trxA"
                     /locus_tag="VEC56_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXV12992.1"
                     /translation="MSQEFEFTDANFDREVLQADRPVLVDFWAPWCGPCRAIAPIVAE
                     ISSEYAGKLKVGKVNTDDNQQIAVRYGIMSIPTILIFKNGKPVEQIVGVGGSPKKTLT
                     TKIDAVLAS"
     gene            5579..7276
                     /locus_tag="VEC56_02190"
     CDS             5579..7276
                     /locus_tag="VEC56_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXV12993.1"
                     /translation="MSRLYLLVLSACLAASSFACGTTDERTARSSHETLADADTSRGP
                     VSGGRIVIGVQQEPEMLSEILNSMATNNMVCNLIFSKFVKYDDHFNLIPDLITEIPTV
                     ENGGVSADHRVYTYHLRSGVRWHDGRPLTSRDVRFTFDIIMDPKVNVESREGWEAVAS
                     LETPDDTTVVFRLQRPYPDFVGETFFDEPVLPEHLLRDSAGERFHAARFHRAPVGSGP
                     FTFKSWTPGSHLELVANADYYGEGPYLDAIIFKFIPNENTLLVQLKTGEIDVFDNANI
                     NFMSQLEAIPGIRIYRTPMLMYEHLDLNVENPVLADRRVRRALSFATNKAEIAERIYN
                     GLVMAAPLDEFAESRYYSPSAAERARFDLAAARRLLHEAGWRDTDNDGIVDRDGVPLK
                     LTISASAGQPNRERTELVLREQYRAVGIDVEIRNYGPTVLYGTYEDGGILKRGSFDIA
                     MYAWLSTPEPSRREALYSATSVPPQGQNHPRFRHDELTRLLEEGSTETDVAKREVIYQ
                     RVQEILVDEAPVIPLFWYTAIDPVTERLQNFRPNATQSADTWNAATWYLGGPQVSSR"
     gene            7401..8360
                     /locus_tag="VEC56_02195"
     CDS             7401..8360
                     /locus_tag="VEC56_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV12994.1"
                     /translation="MLRFFVKRTIAIIPLVLLVSLICFALMQATPGGPVAVLTDNPMV
                     GPEDVARIRANFGFDRPVIVQYGLWLKRVLFHGDFGHSYVTGEPVIEMIWQRLPATLE
                     LMGAAFLLAFLFGLSGGIVAALKRYTKFDSVIMLVTLFGISIPVFWLGLMSMMLFTVK
                     WRLLPSAGMFALGVPFSVLDHLRHLLMPALVLSLLFIASWSRYMRASLSDVLAMNYIN
                     VARAKGATRGSVVFRHAVRNAAIPVLTVIALNLPALFTGSIITETIFAWPGMGRLFYD
                     GLLRQDYTRLMGIIFIASALIAVLNLIADTIYGILDPRIRLAR"
     gene            8323..9297
                     /locus_tag="VEC56_02200"
     CDS             8323..9297
                     /locus_tag="VEC56_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV12995.1"
                     /translation="MAFSTPVLDSPAKGGSRRTVVVQTVHPLRDTLRSSPTRASRGRE
                     HNYFSSVLRRSPLAYAALIVMSLVVLGAVFAPLMSPYHKDKIDLTRQYLPPSLDHPFG
                     TDDLGRDIFVRVMHGGRISLAVGFLAMLVALLIGVAAGGVAGYYGGWVDTVLMRAVDL
                     MLAVPVFFVILFLSSVVSPKISIVAVCLMIGCTQWMEVARLVRAVVMTTKEHEFVDAA
                     RTLGLPDRRIIVRHLLPHTTAPVLVAVTLGLAQAIIIESGLSFLGFGVQPPTPSWGAM
                     LQNAQSYLSDCPWIAIFPGLMICLTVVCCNVLADFLGEAMSPARRAAR"
     gene            9439..10182
                     /locus_tag="VEC56_02205"
     CDS             9439..10182
                     /locus_tag="VEC56_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXV12996.1"
                     /translation="MSSSTLIILFSLLAAAATLAGGMAATLPGRLSHRRLSHIIAFGA
                     GYILAAALVSLLPESLELVASAPLWVLAGYALAHLFEHTFTTHFHFGEETHAEHVVTT
                     RVSTTALVGLSLHAFFDGVAISSGFLVTPALGVLIALAVVLHKVPEGVTISSLMLASG
                     RTRKSAREAALLLAAATLLGAACMSLLGEGLKGIGLALSCGIALYVAATDLMPEINQH
                     PEGRYSISAATGVLLYFGVHALMGRVGLH"
     gene            10184..10516
                     /gene="cutA"
                     /locus_tag="VEC56_02210"
     CDS             10184..10516
                     /gene="cutA"
                     /locus_tag="VEC56_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXV12997.1"
                     /translation="MAGSDYIVVLITAPTMADARRIARLLVEERFAACVNLLPECRSV
                     YRWEDDVVEESEAMMVVKTARELFPALAKRVAEIHPYAVPEVVGLPVVEVAERYRHFL
                     ADGVRREK"
     gene            complement(10522..10974)
                     /locus_tag="VEC56_02215"
     CDS             complement(10522..10974)
                     /locus_tag="VEC56_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV12998.1"
                     /translation="MRTLAIGASAGLILIGAYSLVYACNESKANRDTAAKAAKAPKAA
                     TWYAVEASATTPKVDHVIGHPVGVCKTVEAPIAIEVDVPGEAFQMTRVIHIRGAAVKH
                     PEAPATPVDAVKRTAKVAATLGRAFVTTVGAVWGSLFDVAVQATAALV"
     gene            11096..11914
                     /locus_tag="VEC56_02220"
     CDS             11096..11914
                     /locus_tag="VEC56_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV12999.1"
                     /translation="MKLPAIGVALSGGTAKSVTHVGVVKALVEAGIPISYIAGTSGGS
                     IVGTMYASGMPISTMETVATNMSWRKLVSIRLTRLGFVSSQRIEDFVVETIGKLDFGD
                     LAIPCAVVATNLVTGDTKVFRSGPVARAVRASCSIPQIYLPVEIDGEYFVDGGLSQYL
                     PVETAREMGAEFVLASHLAPVDPSYRRPQNILQLVVQVTGLMARKNFPISEAKADFVV
                     HPNVDAYSSFDFEHAAEMIEIGYDATRRVIDDLKEKWERSGSLGQRIVRKLAGE"
     gene            11921..12736
                     /locus_tag="VEC56_02225"
     CDS             11921..12736
                     /locus_tag="VEC56_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXV13000.1"
                     /translation="MGRPIVLLTDYGSDEFYAGVTRAVLASSSPASAVVDLTHDVPAH
                     DVSRASFLLARSFEYLPAGAVVVAIVDPGVGTARRCLVIEIGDRTLVGPDNGFASDLL
                     AMHAAASFFAIDEAAAQLATGAVARGATFQGRDLFAPVAAAIARGCTPADFGPATDRV
                     VMLRGIPSVSIDGGRVHALGRHIDRFGNLLSDLPRAVLERAFGPDLGRVRARVGNIDA
                     GGLVRTYADGAAGTLIVLLDSWDVVEAAVNGGRACDLLGVARPQDARFELYAP"
     gene            12939..13640
                     /locus_tag="VEC56_02230"
     CDS             12939..13640
                     /locus_tag="VEC56_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV13001.1"
                     /translation="MKRALMTFSLSILGCMLVGSAHAQVGSPPEEAKYFRIPNKFHLF
                     VELGGSLPSNPGVFNDLWNSAFQFGAGGGMNVISWLDVNANFTYASWENNSTDSKSVI
                     GFVGVPDVEGGLITTMTFSGSARFVAVPSARTNPFAEFSVGYYQTSADPLTIEGVLSN
                     TMEDCSGMMIAPALGVQYALADSWGAYAKYSYVYCSSSEFAPGDLLRPQGGGEPAQGD
                     DEIFQTITVGVMLRF"
     gene            13661..14266
                     /locus_tag="VEC56_02235"
     CDS             13661..14266
                     /locus_tag="VEC56_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13002.1"
                     /translation="MRVVVRTIAALGIVAVWSVPPAYAEQPNRSGFSTGFGLGGASVS
                     WTWPDDERRREESAAGNARFAWALKQDVLVGLELWGWTKDYEIGSVPEDVPAQTSVWA
                     ATVAATYFPGNAGFFMRGGIGVGRGRAEVTPPPSVVGFPVSGTESDTGLSVLGAMGYE
                     AGLTPHLSLGGAAHVVYVGIDAAPFDDVFGYGLTVQFNWYW"
     gene            14314..15351
                     /gene="fbp"
                     /locus_tag="VEC56_02240"
     CDS             14314..15351
                     /gene="fbp"
                     /locus_tag="VEC56_02240"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HXV13003.1"
                     /translation="MPPEIPKATQPGVITIERHILMEQEKFPDSSGALTSLLYDIALA
                     GKLIASRTQRAGLADILGSAGHENVQGEEVQKLDVYAQRTIYQLNDHTGRLCCMASEE
                     SKDIIPIPEQYPTGEYVLVFDPLDGSTNIDFNVAVGTIFAIFRRRSKSGPGRMEDVLQ
                     SGREIVAGGYLVYGSSTMLVYTTGHGVNGFTLDPSIGEFLLSHPNVRIPDQAKYYSVN
                     QGYEKYWTEGVRHYTRWLQGEEGSPKSLSLRYIGSMVADVHRTLFGGGVFYYPADTKD
                     PKKPTGKLRLLYEAVPMAYVIEQAGGYGSTGRGPILDVEPEALHQRTPVFVGNRDVVR
                     KAEEFIAKYDR"
     gene            complement(15355..>16192)
                     /locus_tag="VEC56_02245"
     CDS             complement(15355..>16192)
                     /locus_tag="VEC56_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HXV13004.1"
                     /translation="DRDVVDTARRFFEGIGLEVGDIIARSDLYEKPGKEQHAYCLDID
                     RCGDVRVLANVQHDENWAGTMLHELGHAVYDRYIDAELPFLLREHAHVFATEAIAMLF
                     GRLSKDADWIGRMIGVAPPERESLARESERNARLSQLVFARWCQVMVRFERALYANPD
                     GDLNQLWWDLVERYQSVPRPEGRNEPDWAAKIHVVSAPVYYHNYMLGELFASQLDYTI
                     QKNVLHARNGRASFVDQRAVGEFLREKVFAGGKRWRWDELVTRATGEPLNPERFVAQF
                     VH"
BASE COUNT         2789 a         5296 c         5281 g         2826 t
ORIGIN      
        1 agaagtatct cgcgatgtgc aaggagctcc tggccaagac gcgccccggc cgcggccgcc
       61 tgctcttgcc ggtcgacacc atcgtcgcgc aggagatcga cgacaacgcg cccgcgcggg
      121 aagtctccat cgccgacatc cccgaggact ggatcggcgt cgacgtggga cccaagaccg
      181 tcgatctttt caaggcccag atcgcgggtg ccaagacggt gttctggaac ggccccatgg
      241 gcgttttcga aaagccgagc ttcgccgagg gaaccaaggc ggtcgcccgc gccatcgccg
      301 acgcgaccga ccgcggtgcc aagaccatcg tgggaggcgg cgacagtgtg gcggccatca
      361 acttgatggg catcgcgaac cgcttcacgc acatctccac cggcgggggc gcctcgctgg
      421 agttcatgga gggtcgcacg ctgccgggca tcgaagcgct gtgcgacgtc gcggaggtcg
      481 cgggctagct cgcgaggaat ttcagcgcgc ttcatgccac gccccaagct catcgccgcc
      541 aactggaaga tgaacaagac catctccgag gcggtcgcgt tcgcttctga attgcgcgcg
      601 gcgcttcctt ccgtccccgg ccactgcgac ctggtcgtaa tcccgacctt cttcggcgtg
      661 cgccccgtgg tcgaagccct cgcgggggcg cgcgtgtggg tgggcgcgca ggacctccat
      721 tgggagaaga gcggcgcgtt caccggcgag gtggcggccg acatggtcaa ggacgcgggt
      781 gccatgtacg tgctggtcgg tcactccgag cggcgtcacg gcctgggcga aaccgacgcg
      841 gtggtggcga agaaactcgc ggccgcgcag ggggcggggt tgaccccgat tctgtgcgtg
      901 ggcgaaacgc tctccgagcg cgaaagcggg ctgcaagccc acgtggtgga acgccagctc
      961 gcggtggcgt tcacgggcgt gagcccggac gacgcgcgcg cgaccgtgat cgcgtacgag
     1021 ccggtgtggg cgatcgggac cggtcgtacc gcgagccccg acgacgcggt gtctatgcac
     1081 cgcacgatcc ggggctgggt ccaaggacgc tacggcccgg tggtcgccaa ggcgatacgc
     1141 atccagtacg gcggcagcgt caaccccacc aacgcggcgg acctcctgtc gcgggacgag
     1201 atcgacgggc tcctggtggg gggcgcgagc ctggaagccg cctcgttcct gggcatcgct
     1261 cgggccgcgg ggccgcgtta agatccgcgc cggattgact ttcgggggcc tctctggtag
     1321 gctcccgcga cgcggtgacc cccaccgcaa tttttgtttg gcgggcgggc cagcccgccg
     1381 gaaaccaagg atacctcgat gttgttcaac attctgatcg gcctgcacgt catcgtgtgc
     1441 gtcgtgctgg ttctcgtggt cctgctccaa tcgagcaagg gtggaggact ggcgggcgcg
     1501 ttcggcggtg gtggagggca gacgatgttc ggcggtcagg aaacggcgac cttcttgagc
     1561 aaggcgacca cctacctcgc cgtgaccttc atgatcctct cgctcctgct ggcgttcctg
     1621 tccgcgcgcc gcagcgcgcc cggctcgcgc agtctggttc gcgaggccgc cgagcagcag
     1681 cagaacgtga ttccctcggg gcagagcatc gacgagattc tgggaggcgc ggacacgact
     1741 gccgccggcg cggacacgac ctcaccgcgc tgagacgatt gattcaccgg cccaggtggt
     1801 ggaattggca gacacgctat cttgaggggg tagtagacga aagtctgtgc gggttcgagt
     1861 cccgccctgg gcaccaacca cgatacggac tgaggcgccg ccgcgagcgc caaaacgaaa
     1921 tccacctttc gagcgactaa ccacgggacg cccacaacgg gtgtcccgtt ggcgtttttg
     1981 agacaggagg acgaccatga cctacgcgaa gctcctcggc atcgccgccc tgatcggcgg
     2041 cgtcggcacg ggtgcgtccg cccccgccgc ggacctcaag agcacacccg ccgatcagaa
     2101 gtccgtgagc atcaccatct acaacgacaa cctcggcctg gtgaaggacg tgcgcacgct
     2161 ggacttgaag accgggacgc agagcctgtg gttccagggg gtggcggcgc acatcgaccc
     2221 caccacggtg cacattcgat cgatcgatgc gcccaacgcg ttgtccgtga tcgagcagaa
     2281 cttcgagtac gatctcgtgt cgccggagcg attgatggag aagtacatcg gtcagacggt
     2341 ggagctggtc acatcgcgcg agaacaaaga tgacgaaact atccgcgcca agctgatcgg
     2401 catccagggt ggaacggtct accagctcga gaacggcaag atcgccgtca acccgccggg
     2461 acgcgtgatc ctgcccgcgc tgcccgcggg cttgatctcg gagccgtcgc tggtgtggct
     2521 gctggatacc agcaagacca agcacacggt cgaagcgagc tacctcacgg gcggcatcgg
     2581 ctggcgctcg gactacgtgg ccgtgctgtc cgcggacgac agcaaagccg atctctcggg
     2641 ctgggtgacg atcgacaacc agagcggtgc cacgtatgag aacgcgtcgc tcaagctagt
     2701 ggcgggcgac gtccaccggg cgcggcccga gtacgcggcg gtaccccaga tggctgacat
     2761 ggcagttcgc agtctggcca aggaagaaca attccgcgaa gaagcgttct tcgagtacca
     2821 cctgtacacg ctcgaacgcc ccgcgaccgt caaggaccgg cagaccaagc agatctcact
     2881 cttgtcggcc gaaggcgcca aggtgaagaa gaccttcgtc gtgcaaccgc agtacgcgta
     2941 ttggttcaat cgcgcggccg atgtcgaaaa gcccaaggtc ggcgtgtacg tgtcgctcga
     3001 caactcgaaa aagaacggta tgggcatgcc gctgccgaag ggcatcgtgc gcgtctacaa
     3061 gaaggactcg aagagcgagt tgcagttcat cggcgaggac cgcatcgatc acacgcccga
     3121 ggacgaaacc attcgcatca agatgggaga cgccttcgac gtggtggcgg agcgcgtaca
     3181 gaccaacttc gaggtgcttt cgagtgggca cctgtaccgc tcgtcgtaca aggtgacggt
     3241 gcgcaaccac aaggccgagg acattacggt ccaggtagtc gaattgctga gcggcgactg
     3301 ggccatcacg acgtcgtcgt acccgcacgt caaggaggcg tcccatcgcg cgcgcttcga
     3361 cgtcccggtc aagtcgaagg gttcggccga gctgacctat accgccgaga tccggtatta
     3421 gcccgtgtta ttggaggctt gaggacgcca cgatccgggc cacattggtg tttacatccg
     3481 cgcgcgcggc gtattagatt cgccgcggct ttcggccagt tacgggatca caccaggggg
     3541 caacttggat cagaccagcg gtcttgacgg caacgcactc gttctagcgg tcggcgcact
     3601 cggcgtcgtc ggcatcgtca tcggggtgct cgcgctcgtg cggttgcgcg ccatctcggg
     3661 tcgcttctcg tgggtgaccg gcaaggacgc cggcggcgtc gacaccctgc ccgcgctgct
     3721 cgcagctgtc gaaggcagcc agcgcgacat ccagaatctc cagaccgaat tcaagctggc
     3781 gtcgatcgag tcgcgcacgc acttcaagcg cctgagcatc gtgcgctaca acgcgttcga
     3841 gggtgtggcc ggacaacaga gctactcgtt gtgcctgctc gatgagaacc agaacggaat
     3901 cctgatctcg aacctcgtcg gcaccaactt cagtcgcagc tacgcggtcg aagtgaagac
     3961 gggggagccc gcccgtccgc tcggggaaga agagaagcag gcgctcgcct ccgcgatcaa
     4021 gaacgggaac tagtcctact cttccggctc ttcgcgctgc tgcttgctgt agttctccag
     4081 gatccgcttc tcggtcgggg acaggttctc gtagcccttc gccgatatct tgtcgaggat
     4141 cgagtcgatg ttgtccgccg aagcgcgcgg gccacgccgc gtgccgaagc cgacgcgggg
     4201 gcgccaggtg ggaccgcgac gacgggcacc tcccgtccag cccaggcgcc gcgcgacctc
     4261 gtcgcggaag aacgtcagcc ccgccaccat ccccccgata tgggcgaagt acgcaatacc
     4321 cgctttgccg gcgatcccgt tggcgagctg cagcaccgcc aggccgatca ccaggtactt
     4381 gatcttgatc ggcaccaaga agtagagata cacatacgca tcgggaaaga gcatcgcggc
     4441 cgcgagatag aggccgaaga tggcgccgct agcgcccacc acgctggcgt tgtaatcgaa
     4501 catcatgtgg agcagcgcgc cgcccacccc gcagacgacg tagtagcgca ggaatcgttt
     4561 gcgcccccac gctccctcaa ccatgttgcc gaggaaaaat aggacgatca gattgaagaa
     4621 gaggtgccag aagtccccgt ggaggaacat ataggtcacg agctgccaga gatacagctt
     4681 ctcgaccacg tcgcgcgggg tcagagcgag aatgccttcg acgctcggga acaggttctg
     4741 gacgaagaac atcgccacgg tggcgaccat gatgcgtccc acagcggagc ccagaaacgg
     4801 tggtcccgag tacggcggtt gtggacggtg agagcgcgac gtcataacgg ttgggcgcct
     4861 cgaggcgccg cggtcaacat aaccacaccg acgcgggcgg ggcaagcgtc gccacgcccg
     4921 cgcgggcggc gcagcccggg cacgatggcc gggtgtaagg gcgccgggtc ggccgcgtcc
     4981 aagaccgcgg ccagggcgcg gaaagccgct ttacaggcgc ttcacctcgc gcgataatcc
     5041 gccggcgcga tgagcgccgc cagggatacc cggggagaat cgagcgcacg cgccgcgccc
     5101 ccccgccatc tgggaatgac aacacgatcg aggaggactt gatgtcgcag gaattcgagt
     5161 ttaccgacgc caacttcgac cgggaggtcc tgcaagcgga ccgccccgtg ctcgtggatt
     5221 tttgggcgcc gtggtgcggc ccgtgtcgtg cgattgcacc catcgtcgcc gagatatcct
     5281 cggagtacgc gggcaagttg aaggtcggta aggtgaacac cgacgacaac cagcagatcg
     5341 cggtgcgata cgggatcatg agcattccga ctatcctgat cttcaagaac gggaaacccg
     5401 tggagcagat cgtcggggtc gggggcagcc ccaagaagac cctgacgacg aagatcgacg
     5461 ccgtgctcgc gagctgatcc aaacgcacgg agcacgcccg gccgaggccg gccccgtcgg
     5521 ggccggcctt tttgttgacc gacccggcgc ggcgtggaac ttgcaataac ctccgggtat
     5581 gtcccggttg tacctcttgg ttctctcggc gtgcctcgcc gcgtcctcgt tcgcctgcgg
     5641 aacgaccgac gagcgcaccg cgcgctccag ccacgagacg ctcgctgacg ccgacacctc
     5701 gcgcggcccc gtctccgggg gccggatcgt catcggcgtc cagcaggagc cggagatgct
     5761 gagcgagatc ctgaattcga tggcgaccaa caacatggtc tgcaacctga tcttctcgaa
     5821 gttcgtcaag tacgatgacc acttcaatct cattccggac ttgatcacgg agatcccgac
     5881 cgtcgagaac ggcggtgtct ccgcggacca tcgcgtgtac acgtaccatc tgcgctcggg
     5941 cgtgcgctgg cacgacggcc gcccgctcac cagccgcgac gttcggttca ccttcgatat
     6001 catcatggat cccaaggtca atgtggaatc gcgcgagggg tgggaagcgg tggcctcgct
     6061 ggaaacgccc gatgacacca cggtggtgtt ccggctccag agaccgtatc cggacttcgt
     6121 cggagagacc ttcttcgacg agccggtgct gccggaacac ctcctgcgcg actcggccgg
     6181 cgagcgtttc cacgccgctc gttttcatcg cgcgccggtg ggctccgggc cgttcacgtt
     6241 caagagctgg acgccgggct cgcacctgga gctggtcgcc aacgccgact actacggtga
     6301 gggaccttac ctcgacgcga tcatcttcaa gttcattccc aacgagaata cgcttttggt
     6361 acagctcaag accggtgaga tcgacgtctt cgacaacgcc aacatcaact tcatgtcgca
     6421 gttggaggcg atccccggca ttcggatcta ccgcaccccg atgctcatgt acgagcacct
     6481 ggacctcaac gtggagaatc ccgtactcgc cgatcggcgt gtgcgtcgtg cgctgagctt
     6541 cgccaccaac aaggcggaga tcgcggagcg catctacaac ggcctggtga tggcggcccc
     6601 gctcgacgag ttcgcggagt cgcgctacta ctcgccctcg gccgccgagc gagcccgctt
     6661 cgatctggcc gccgcgcgcc ggctgttgca cgaggcgggg tggcgcgaca ccgacaatga
     6721 cggaatcgtc gaccgggacg gcgttccgct caagctcacc atttccgcca gcgccggcca
     6781 gccaaaccgt gaacgcaccg agctggtgct gcgcgagcag taccgcgcgg tgggcatcga
     6841 cgtcgagata cgcaactacg gcccgacggt gctgtacgga acctacgaag acggcggcat
     6901 cctcaagcgc ggttccttcg acatcgcgat gtacgcgtgg ctctcgaccc cggaaccgtc
     6961 gcggcgcgag gccctgtact cggccaccag cgtcccgccg caggggcaga accacccgcg
     7021 cttccgccac gacgagctca cgcgcctgct cgaggaaggc agcacggaga ccgacgtcgc
     7081 caagcgcgaa gtcatttatc agcgcgtgca agagatcctg gtcgacgaag cccccgtcat
     7141 cccgcttttt tggtataccg cgatcgatcc ggtgaccgag cgcctgcaga acttccgccc
     7201 caacgcgacc cagtcggccg acacttggaa cgcggccacc tggtacctgg gcgggcctca
     7261 ggtctcctca cgctagcccg ccctcggcgc tctcgtcggc caacccgtag ttggtacaaa
     7321 actcgcagtc ccgcctggga agcccggcgt cagccaccgc cggacaccca taactcatta
     7381 ttgttaataa acttatgtcg atgctaaggt tcttcgtaaa gcgcacgatc gcgatcatcc
     7441 cgctcgtact gctggtgtcg ctgatctgtt ttgcactgat gcaggcgacc cctggcgggc
     7501 cggtggccgt gctgaccgat aatcccatgg tcggccccga ggacgtcgcg cgcatccgcg
     7561 ccaacttcgg cttcgaccgc cccgtgatcg tccagtacgg cttgtggctc aagcgcgtgc
     7621 tttttcacgg agacttcggg cactcgtacg tcaccggcga accggtgatc gaaatgatct
     7681 ggcagcgcct tcccgccacc ctcgagctca tgggggctgc gttcctgctg gcattcctgt
     7741 tcgggctctc gggcggcatc gtcgcggcac tcaagcgcta caccaagttc gactccgtca
     7801 tcatgctggt gacgctgttc ggcatctcga tcccggtgtt ctggctcggg ctgatgtcga
     7861 tgatgctgtt cacggtgaag tggcgcctgc tcccgtccgc gggcatgttc gcgctgggcg
     7921 tgccgttctc ggtcctggat cacttgcgcc acctgttaat gccggcgttg gtcttgtccc
     7981 tcttgttcat cgcgagttgg agtcgctaca tgcgcgcgag cctctccgac gtactggcca
     8041 tgaactacat aaacgtcgcg cgcgccaagg gcgccacgcg cggctcggtg gtgttccgcc
     8101 acgcggtgcg caacgcggcc attccggtgc tgacggtgat cgcgctgaat ctgcccgcgc
     8161 tgttcaccgg gtcgatcatc acggagacga tcttcgcgtg gccgggcatg ggacggttgt
     8221 tctacgacgg actgctgcgg caggactata cgcggctcat gggcatcatc ttcatagcgt
     8281 cggcgctgat cgccgtgctg aacctaatcg cggacacgat ctatggcatt ctcgaccccc
     8341 gtattagact cgcccgctaa aggcggctcg cggcgcacgg tggtcgtgca gacggtgcac
     8401 ccgctgcgcg acacgctgcg ctcgtcaccc acgcgcgctt cacgcggccg cgagcacaac
     8461 tacttcagct cggtgctgag gcgaagcccg ctggcgtacg cggccctcat cgtcatgagc
     8521 ctggtcgtgc tgggggcggt gtttgcgccc ctgatgagtc cgtatcacaa ggacaagatc
     8581 gacctcacgc gccagtacct gccgccctcg ctcgaccatc ccttcggaac cgacgacctc
     8641 gggcgcgaca tcttcgtgcg cgtgatgcac gggggccgca tctcgctcgc ggtgggcttc
     8701 ctggcgatgc tcgtcgcctt gctgattgga gtcgcggcgg gaggcgtcgc cggctattac
     8761 ggcggatggg tcgacaccgt cttgatgcga gccgtagacc tcatgctggc ggtccccgtc
     8821 ttcttcgtga tcctgttcct ctcgagcgtg gtgtcgccga agatatcgat cgtcgcggtc
     8881 tgcttgatga tcggttgcac gcagtggatg gaagtggcgc gcctcgtgcg cgcggttgtg
     8941 atgaccacca aggaacacga gttcgtggac gcggcccgga ccctgggtct gcccgaccgg
     9001 cgcatcatcg tccgccacct gttgccccac acgaccgcgc ccgtcctggt cgccgtgacg
     9061 ctcggtctcg cccaggcgat catcatcgaa agcggtcttt cgttcctggg ctttggcgtc
     9121 cagccgccca cgccgagttg gggtgccatg ttgcaaaacg cgcagagcta cttgagcgac
     9181 tgcccgtgga tcgcgatctt ccccggcctg atgatttgcc tgaccgtggt gtgctgcaac
     9241 gtcctggccg attttctggg cgaggccatg agccccgcac gccgcgcagc ccgctgaacg
     9301 cccgcccgcc gcacccgcct cgaattcacg ttgatgtccc cccgggcgtg gggtattctt
     9361 caccggcccg acgcgctatc ttcttagaaa caattggatt acatagtgat tcgggagggc
     9421 cgacgcggcc cgcacgatat gagttcctcc accctcatca tcctgttctc gcttctcgcc
     9481 gcggccgcca cactcgcggg cggcatggcg gcgacgctgc ccgggcgcct gtcccaccgc
     9541 cgcttgagcc atatcatcgc gttcggcgcc ggatacatcc tggctgcggc tctagtgagc
     9601 ctcttgccgg agagcctgga actggtggcg tcggcgccgc tgtgggtgct cgccggctat
     9661 gcgctcgcgc acctcttcga gcacaccttc accacgcatt tccatttcgg cgaagagacc
     9721 cacgccgagc acgtcgtcac gacgcgcgtt agcacgactg cgctggtggg cttgtcgttg
     9781 cacgcgttct tcgacggggt tgcgatttcg tccgggttcc tggtcacccc cgcgctcggc
     9841 gtgctgatcg ccctggcggt cgtcctgcac aaggtcccgg aaggcgtgac catctcgtcg
     9901 ctcatgctcg cgtccggtag gacgcgcaag agcgcgcgcg aggccgccct gctgctcgcg
     9961 gccgcgaccc tgctcggggc ggcgtgcatg tcgctcctgg gcgaggggct caaggggatc
    10021 ggattggcgc tctcgtgcgg catcgccctc tacgttgcgg ccaccgacct catgcccgag
    10081 atcaaccagc atcccgaggg gcgctactcg atcagcgcgg cgaccggcgt gctgctatac
    10141 ttcggagtcc acgccctgat ggggcgcgtg gggttgcact gacatggcgg gatccgacta
    10201 cattgtcgtt ctcattacgg cgccgaccat ggcggacgcg cggcgtatcg cgcggctgct
    10261 ggtggaggaa cgcttcgccg cgtgcgtgaa cctgcttccc gagtgccgaa gcgtctatcg
    10321 gtgggaggac gatgtggtgg aggagtccga ggcaatgatg gtggtcaaga cggcccgaga
    10381 gctgtttccg gcgctggcga agcgggtggc ggagattcac ccctacgccg tccccgaggt
    10441 agtggggcta cccgtggtgg aagtcgccga gcgctaccgg cactttctgg ccgacggcgt
    10501 gcggcgcgag aagtagtgtc tctagaccag ggccgcggta gcctggaccg cgacgtcgaa
    10561 gagcgatccc cacaccgcac cgaccgtcgt gacgaacgcg cggccgagtg tcgcggccac
    10621 cttcgcggtc cgcttcacgg catcgaccgg ggtcgcgggc gcctccgggt gcttcaccgc
    10681 cgcgccgcgg atgtgaatca cgcgcgtcat ctggaaagcc tcgccgggga cgtcgacttc
    10741 gatggcgatg ggggcctcga cggtcttgca gacgccgacc gggtggccga tcacgtggtc
    10801 gaccttgggc gtagttgcgc ttgcctcgac cgcgtaccac gtggcagctt tgggtgcctt
    10861 cgcggccttg gccgcggtgt cgcggtttgc cttggactcg ttgcaggcat atacaagtga
    10921 ataggcaccg atcaggatca acccagccga tgcaccaatc gccaacgttc tcatatcacc
    10981 cctccttgtc gactacatac gcggggccgg cgcgcccggt tcccaacgat ctcccatggt
    11041 tgacccttag acgccctttt tggcggaatc gttgcaattt ttcccaggag ccgcgatgaa
    11101 actgcccgcc atcggagtgg ccctcagtgg tggcacggcc aaatcggtga cgcacgtggg
    11161 ggtggtcaag gcactggtcg aagccggaat acccatttca tacatagccg ggaccagcgg
    11221 cgggagtatc gtcgggacga tgtacgcctc gggtatgccg atctcgacca tggaaacggt
    11281 cgcgaccaat atgagctggc ggaagctggt cagcatccgg ctcacccgat tgggattcgt
    11341 ctcgtcccag cggatcgaag acttcgtcgt ggagaccatc ggcaagctcg atttcgggga
    11401 ccttgcgatc ccgtgcgcgg tcgtcgccac caacctcgtg accggcgaca ccaaggtctt
    11461 ccgcagcggc ccggtcgcgc gcgcggtacg cgccagttgc agcattccgc agatctatct
    11521 gccggtcgag atcgacggcg agtatttcgt cgacggcggt ctctcgcagt acctgccggt
    11581 cgagaccgcg cgcgagatgg gtgcggagtt cgtgctggcg tcgcacctgg cgccggtcga
    11641 ccccagctac cggcgcccgc agaacatcct gcagctggtg gtccaggtga ccgggctcat
    11701 ggcgcgcaag aattttccga tatcggaagc caaggcggac ttcgtcgttc accccaacgt
    11761 cgacgcgtac agctcgttcg atttcgaaca cgccgccgag atgatcgaga tcggctacga
    11821 cgctacacgg cgggtgatcg acgacttgaa agagaagtgg gagcgctccg gcagcttggg
    11881 ccagcgaatc gtgcgcaagc tcgctggaga gtagcgcggt atggggcggc ccatcgtcct
    11941 gctcacggac tacgggagcg acgaattcta cgccggcgtg acgcgcgcgg tactggcctc
    12001 gtcgtcgccc gcgtccgccg tcgtcgacct cacccacgac gtccccgcgc acgacgtctc
    12061 gcgcgcgagc ttcttgctcg cgcggtcgtt cgaatatttg cccgcgggtg cggtggtggt
    12121 cgcgattgtg gaccccggtg tggggaccgc gcggcgctgc ctcgtgatcg agatcggcga
    12181 ccgcactctg gtgggacccg acaacggctt cgccagcgac ctgcttgcga tgcacgccgc
    12241 cgccagcttc ttcgccatcg acgaagccgc ggcgcaactg gccaccggcg ccgtagcgcg
    12301 gggcgccacc ttccagggcc gcgacttgtt cgctccggtt gcggccgcga tcgcccgcgg
    12361 atgcacgccc gccgacttcg ggcccgcgac cgaccgcgta gtcatgttgc gcggaatccc
    12421 cagcgtctct atcgacggcg gacgcgtgca cgcgctgggg cgccacatcg atcgcttcgg
    12481 caatttactt tcggatctgc cgcgggcggt tctcgagcgc gcgttcggac cggaccttgg
    12541 ccgcgtgcgc gcccgcgtcg gcaacatcga cgccggcggg ctcgtgcgga cctacgccga
    12601 cggagcggcc ggcacgctca tcgtgctgct cgacagctgg gatgtggtgg aggccgcggt
    12661 caacggcggc cgcgcgtgcg acctcttggg cgtggcgcgc ccccaggacg cgcgtttcga
    12721 gctctacgcc ccctgagcgg gtccccgtcg cgcgggcact tttgggcttc aagcgcacgc
    12781 ggcaatcggc tatgctttcg cccctgaaaa aaaacgcgag ggtcacgccc agaccggacc
    12841 ggcccgcctc gggccgggtc tcccgatcca tcacgaaaga tcattcgaaa cggacggcac
    12901 gcgggtcgcc gcgccgaatc agtaggagaa cggaatcgat gaaacgcgcg ttgatgacat
    12961 tcagcctctc gatcctggga tgcatgctgg tggggtcggc ccacgctcaa gtcgggagcc
    13021 cgccggaaga agcgaagtac tttcgcatcc cgaacaagtt ccacctcttc gtcgaactcg
    13081 ggggctccct gccgagcaac ccgggggtgt tcaacgatct gtggaactcc gcctttcagt
    13141 tcggcgccgg cgggggcatg aacgtaatct cctggctgga cgtcaatgcg aatttcacct
    13201 acgcgtcgtg ggagaataac tcaaccgatt cgaagtcggt gatcggcttc gtcggtgttc
    13261 cggatgtcga gggcgggttg atcaccacca tgacattcag cggctccgcg cgctttgtcg
    13321 ccgtgcccag cgcgcgtacc aatccgttcg ccgagttcag cgtgggctac tatcagacgt
    13381 cggccgaccc actcacgatc gaaggcgtgc tgtccaacac catggaggac tgcagcggaa
    13441 tgatgatcgc gcccgcgctg ggagtgcagt acgccttggc cgactcgtgg ggcgcgtacg
    13501 cgaagtactc gtacgtttac tgctcgagca gtgagtttgc tccgggtgat ctcctgcgcc
    13561 cccaaggagg cggcgagccc gcccagggcg acgacgagat cttccagacc atcactgtcg
    13621 gtgtcatgct gcgcttctga tcgaacgcgc ccggaagccc atgcgtgtcg ttgttcgcac
    13681 gatcgccgcg ctcggaatcg tcgcggtgtg gtccgtgccg ccggcatacg cggagcagcc
    13741 gaaccgaagc ggcttctcga cgggcttcgg cttgggtggg gcgtcggtgt cgtggacctg
    13801 gcccgacgat gagcgtcggc gggaggaatc ggcggccgga aacgcgcgct tcgcctgggc
    13861 gctcaaacag gacgtgctgg tcggcctcga gctctggggc tggaccaagg actacgagat
    13921 cggcagcgta cccgaggacg ttcccgccca gacgtcggtg tgggcggcca ccgtcgcggc
    13981 gacgtatttc cccggcaacg ctggtttctt catgcgcgga ggaatcggcg tcggtcgcgg
    14041 tcgcgccgag gtaaccccac cgccctcggt ggttggcttc cccgtgagcg gaaccgagtc
    14101 ggacaccggc ctgtcggtgc tgggcgcgat gggctacgaa gcggggctga cgccgcacct
    14161 gtcgctggga ggggccgcgc acgtggtcta tgtcggcatc gacgcggcgc ccttcgacga
    14221 cgtgttcggc tacggactga cggtccagtt caattggtac tggtagcgcg cggcgcgcga
    14281 cgtgctacca tcacaccaca tcgaggtcga agcatgccac ccgaaatccc caaggcaacg
    14341 cagcccggcg tcatcacgat cgagcgccac atcttgatgg agcaggagaa attccccgat
    14401 tcgtcgggcg ctctgaccag tctcctctac gacatcgcgc tggccggcaa gctcatcgcg
    14461 agccgcacgc agcgcgcggg cctcgccgac attctgggat cggccggcca cgagaacgtg
    14521 cagggggagg aagtgcagaa gctcgacgtg tacgcgcagc gcaccatcta tcagctcaac
    14581 gaccacaccg gccgcctgtg ctgcatggcc tcagaagagt cgaaggacat catccccatc
    14641 cccgagcagt atccgaccgg agagtacgtg ctcgtgttcg acccgctcga cggttcgacc
    14701 aacatcgatt tcaacgtcgc ggtgggaacg atcttcgcga tcttccgccg gcgctcgaag
    14761 tcgggaccgg ggcgcatgga ggacgtgctg cagagcgggc gcgagatcgt cgccggcggc
    14821 tatctggtgt acggatcgag cacgatgctc gtgtacacga cgggccacgg cgtcaatgga
    14881 ttcacgctcg acccgtcgat cggcgagttc ctgctctcgc acccgaacgt gcgcatcccc
    14941 gaccaggcga agtactacag cgtcaaccag ggctacgaga aatactggac cgaaggcgtg
    15001 cgccactaca cgcgctggtt gcagggcgaa gaggggagtc ccaagtcgct ctcgctccgg
    15061 tatatcggat cgatggtggc cgacgtccat cgcacgctct tcggcggcgg cgtgttctac
    15121 taccccgcgg acaccaagga ccccaagaag ccgaccggca agctgcggtt gctgtacgaa
    15181 gcggttccga tggcctacgt gatcgagcag gcggggggct atggatccac tgggcgcggt
    15241 cccatcctcg acgtcgaacc ggaggcgctc caccagcgca cgccggtctt cgtcggcaac
    15301 cgcgacgtgg tgcgcaaggc cgaagagttc atcgccaagt acgaccgttg attgtcagtg
    15361 cacgaactgc gcgacgaagc gctcggggtt gagcggctcg ccggtggcgc gcgtgacgag
    15421 ttcgtcccac cgccagcgct ttccgcccgc gaacaccttc tcgcgcagga attccccgac
    15481 cgcccgctgg tccacgaaag aggcgcgccc gttgcgcgcg tgcaacacgt tcttttggat
    15541 cgtgtagtcg agctgcgagg cgaacaactc tcccagcatg tagttgtgat agtacacggg
    15601 cgcggaaacg acgtggatct tcgcggccca gtcgggttcg ttccgccctt cgggacgcgg
    15661 cacggactgg tagcgctcga cgaggtccca ccagagttgg ttcaaatctc cgtccgggtt
    15721 cgcgtagagg gcgcgctcga accgcaccat cacctgacac caacgggcga agaccagctg
    15781 cgacaagcga gcgttgcgct ccgattcgcg ggcgagcgac tcacgctcgg gcggcgccac
    15841 gccgatcatg cgaccgatcc agtccgcgtc cttggacagg cggccgaaca gcatcgcgat
    15901 cgcttcggtg gcgaagacgt gcgcgtgttc gcgcagcagg aaggggagct cggcgtcgat
    15961 gtagcggtcg tagaccgcgt gcccgagctc gtggagcatg gtgccggccc agttctcgtc
    16021 gtgctggacg ttggcgagca cacgaacgtc gccgcaccgg tcgatatcga ggcagtaggc
    16081 gtgctgctcc ttcccgggct tctcgtacag gtcgctacgc gcgatgatat cgccgacctc
    16141 caggccgatg ccctcgaaga accggcgcgc ggtatccacc acgtcgcgat cg
//
LOCUS       DASPGK010000081         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_488940, whole genome shotgun sequence.
ACCESSION   DASPGK010000081 DASPGK010000000
VERSION     DASPGK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488940"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="VEC56_02250"
     CDS             complement(<1..1797)
                     /locus_tag="VEC56_02250"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HXV13005.1"
                     /translation="MRRSVSIAALTLVGVWMPAADPAGAADVTLSLRFRDSGGTQLSV
                     RAGVFAGDVSYPTGNPGANLYQSRGDRSYFYTGGNVSVVVPAGPVTIRAGRGFEFEVL
                     DTTLTVTSSRTLTFRLNRIINMKNLGWYSGDTHVHISHPPVVYSLDAQDLALVARAEE
                     LNFINSMEESEYFTGTIDPVSTSDRIVFFSKEQRNAHFSHLSILGMKQWIYDSAGCDD
                     ENVACGSTLDASIYAQAHAQPGEIAVIATHPFSTFDHFDIEGWPGVGMWRGMPVDLAA
                     GTVDAMDLLCYTSAVPPAGVEPYFQALNAGFRLPPAAGTDCGLGSGTSGPAGGFRVYV
                     DPVGTFTMDSWIAGLKAGRSFVTNYPLFTAFNVEGAVPGDVLTVNEATLSGTVSVRCR
                     LPIHRVEIWGDTGLLDSMTPPNGTAKSFTASFDVASAGLTWVVARVLGLASGWHAIPA
                     GGLFAQTAPIYLERSTAMRDPETRGVSYTRAQAAEYFLVQVDETETVFNALGSFPNGS
                     RESFDLAIASARAFYQTLAGTPTDVGSPALPEAWALRNVWPNPFAGDVHIDYTSPDGG
                     GEHTVAIYDVAGRVVNVLFSGRRDGGDHRLGWD"
     gene            2062..>3564
                     /locus_tag="VEC56_02255"
     CDS             2062..>3564
                     /locus_tag="VEC56_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HXV13006.1"
                     /translation="MKSFVSKAITVAIVALVAWLPACSRKSKQAEEAAELSFTHIPQD
                     SLLIQMARLAPVEIVYDATILSPPEKQALSRLVEVARIFDEIFLHQVWEGNVAMRDQL
                     AAAAAVDGPNKRLAQDLLYFFNLNSGPWLRLEHDTPFIGSTPKPAGAGYYPLDMTKEE
                     LEAYIAANPDQKDAMTGYFSRVARGADGTLHAVPYSEAYGSYLRDASRLMNEAADILT
                     AESSKAQLANGVDYTTLATFLRSRAAAFQSNDYFQSDMDWMDIKDNILDVTIGPYEVY
                     EDALFNYKASFEAVIAMRNPTDSKKLDEVKHYLPAMERNLPIPNEFKNPNRGTDSPIA
                     VVDVIHVGGDMGKGVHAIAYNLPNDERVREAKGSKKVMLKNISRAKYDKILMPIASLV
                     LTPEQLPYVNFDSFFGSSVMHELSHGLGPGTITLPDGSKTTVNLALKTLYSPLEECKA
                     DVMGLYNTEFLVAKGFYTKEELDQAYVVFLPGFFRAIRWGLHEAHARGNMI"
BASE COUNT          687 a         1159 c         1149 g          569 t
ORIGIN      
        1 gtcccagccc agccggtgat cgccaccgtc gcggcgtcct gagaacagca cgttgaccac
       61 gcggccggcc acgtcgtaga tcgcaaccgt gtgctcgcct ccgccgtccg gactcgtata
      121 gtcgatgtgc acgtcgcccg cgaatgggtt cggccacacg ttgcgaagcg cccaggcttc
      181 gggcaaggcc ggcgacccga cgtcggtcgg cgtgccggcc agggtttgat agaacgcgcg
      241 cgccgacgcg atcgccaaat cgaacgactc gcgcgacccg ttcgggaagg agcccagcgc
      301 gttgaagacg gtctcggttt cgtcgacttg aaccaggaag tactccgcgg cttgcgcccg
      361 cgtgtacgaa acgccgcgcg tctcgggatc gcgcatggcc gtgctgcgtt cgaggtagat
      421 gggtgcggtc tgcgcgaaca gcccgcccgc cggaatggcg tgccaccccg acgccaggcc
      481 gagaacgcgc gcgaccaccc acgtgagtcc cgcgctcgcg acgtcgaagg atgcggtgaa
      541 cgacttggca gtaccgttgg gtggggtcat gctatccaag agtccggtgt cgccccagat
      601 ctcgacgcga tgaatgggca gccggcaacg aaccgacacc gtgccgctta gcgtcgcttc
      661 gttgaccgtg aggacatcac cgggcaccgc gccctcgaca ttgaacgcgg tgaacagcgg
      721 gtagttggtg acgaacgagc gtcccgcctt caaccccgcg atccacgagt ccatcgtgaa
      781 cgtgccgacc gggtcgacgt agacacgaaa cccgcccgcc gggccggagg tgccggaccc
      841 cagcccgcag tccgtgcccg ccgcgggtgg gaggcgaaag cccgcgttga gcgcctggaa
      901 gtacggctcg acgccggccg gcggcaccgc gctcgtgtag cacaagaggt ccatggcgtc
      961 gaccgtaccc gccgcgagat cgaccggcat gccgcgccac attcccacac ccggccagcc
     1021 ctcgatgtcg aagtggtcaa aggtcgagaa gggatgcgtc gcgatcaccg cgatctcgcc
     1081 gggctgcgcg tgcgcctggg catagatgga cgcatccagc gtactgccgc acgccacatt
     1141 ttcgtcgtcg caaccggcgg agtcgtagat ccactgcttc atccccagaa tcgacaggtg
     1201 cgagaagtgc gcgttgcgct gctccttgct gaagaaaacg atgcgatccg acgtgctgac
     1261 cgggtcgatc gtgcccgtga agtactccga ctcctccatc gaattgatga agttcaactc
     1321 ctcggcgcgc gcgaccagcg ccaggtcctg cgcgtcgagc gagtagacga cgggtgggtg
     1381 cgagatatgg acgtgggtgt ctcccgaata ccaccccaga ttcttcatgt tgatgatgcg
     1441 attcagtcgg aaggtaaggg tcctcgacga ggtgacggtc agcgtcgtgt cgagcacctc
     1501 gaactcgaaa ccgcggccgg cgcggatggt gaccggcccc gccggcacga cgacggaaac
     1561 gttaccgccg gtgtagaagt agctccgatc gccccgcgac tggtacaggt tcgctcccgg
     1621 gttgccggtg ggatacgaga catcgccggc gaacacaccc gcgcgcacgg agagctgtgt
     1681 gcccccggag tctcggaacc ggagcgaaag ggtgacgtcg gccgcgccgg ccggatcggc
     1741 ggcgggcatc caaaccccga cgagtgtcag ggcagcgatg gagacgcttc gtcgcatggt
     1801 gcgcgctggg tacaggggaa gaggggtgga taatacgcca atcgagcccg cggaacaagt
     1861 gagtgcagtt tggccatacg gatgcggggc ggcggcgccc ccgagtgcca tcccgaccgc
     1921 tggcgggtgc gcggaccgcc tttacaactg gaaccggcaa ggcccataat acccgctcac
     1981 gctagatgag gaggcagggg ccgtcccccg aacccggccg gacacgcgtt cttcggagcg
     2041 ccatcccctg aaggagaatc gatgaaatcg ttcgttagca aggccatcac cgtggcgatc
     2101 gtcgcgctgg tcgcgtggct ccccgcgtgc agcaggaagt cgaagcaagc ggaggaggcc
     2161 gcggagttga gctttaccca catccctcaa gattctttgc tcattcagat ggcgcgcctg
     2221 gcgccggtcg agatcgtgta cgacgccacc atcctgtcgc ccccggagaa gcaggcgctc
     2281 tccaggctgg tcgaagtggc tcggatcttc gacgaaatct tcctgcacca ggtgtgggag
     2341 ggcaacgtcg cgatgcgcga tcagctcgcg gccgcggccg cggtcgacgg gcccaacaag
     2401 aggctggcgc aagacctgct gtacttcttc aatttgaatt cgggtccgtg gctgcgcctg
     2461 gagcacgaca cgccgttcat cggttccacg ccgaaacccg cgggcgccgg ctactacccg
     2521 ctcgatatga ccaaggagga gctcgaggcg tacatcgccg cgaatcccga ccagaaggac
     2581 gcgatgacgg gctacttctc gcgcgtcgcg cgcggggcgg acggcacgct gcacgcggtt
     2641 ccctacagcg aggcgtacgg gtcgtacttg cgcgacgcat cgcggctcat gaacgaggcc
     2701 gccgacattc tcaccgccga gtcgtccaag gcgcagcttg cgaacggtgt ggactacacg
     2761 accctcgcaa ccttcctgcg cagccgcgcg gcggcgtttc aatccaacga ctacttccag
     2821 agcgacatgg actggatgga catcaaggac aacattctgg acgtcaccat cggtccctac
     2881 gaagtctacg aggacgccct gttcaattat aaggcgtcgt tcgaggccgt catcgcgatg
     2941 cgcaatccga ccgacagcaa gaagctggac gaggtgaagc actatttgcc cgcgatggag
     3001 cgcaacctcc ccatccccaa cgaattcaag aaccccaatc gcggcaccga ttcgcccatc
     3061 gcggtcgtcg acgtgatcca cgtcggcggc gacatgggca agggcgtgca cgcgatcgcg
     3121 tacaacctgc ccaacgacga acgcgtacgc gaggccaagg ggtcgaagaa agtgatgctc
     3181 aagaacatct cccgcgccaa gtacgacaag atcctgatgc cgatcgcgag cctcgtgctg
     3241 acgccggagc agctcccata cgtgaacttc gatagcttct tcgggagttc ggtcatgcac
     3301 gaactctccc acggcctggg accgggcacg atcacgctcc ccgacggatc caagacgacg
     3361 gtgaacctag ctttgaagac gctttactcg ccgttggagg agtgcaaggc cgacgtcatg
     3421 ggtctctaca acaccgagtt cctggtggcg aaggggttct acaccaagga agagttggac
     3481 caggcctacg tcgtgttcct gcccggcttc ttccgtgcca tccgctgggg cctgcacgaa
     3541 gcgcacgcgc gcggcaacat gatc
//
LOCUS       DASPGK010000082         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_371480, whole genome shotgun sequence.
ACCESSION   DASPGK010000082 DASPGK010000000
VERSION     DASPGK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371480"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1280)
                     /locus_tag="VEC56_02260"
     CDS             complement(126..1280)
                     /locus_tag="VEC56_02260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13007.1"
                     /translation="MMHRILILDNEFEMGGKEKSLFQFVARADRSRFHFAVCCLKEGG
                     YFKSKTEAMGVPFYDGLQRHRFDAFAFLGLERVLRIERPGLIYTFSHPNTVIFSYLAK
                     LRGLVDRVVVSYHAMGDIGGTRQVLPYLRPLLRRADALLAVAEVQKDYLVRDEGLPRE
                     HIRVIHNGVDTSTFRPATTEERASGRRELGIDDRDVVLVTVASLKALKRIDALLAAAA
                     RLHGAGAPVRVLLVGEGPERSALESLARQLGIAERTIFAGLRDDVDRLLRAADLFVLS
                     SRTEAFPNVVLEAMSAGLPVVATDVGSVREMVEPGMSALVVPPGDDDALAAALAELVS
                     SPDRRRAFGHRGREIAMERFRIEAMCAAREALFDELLTRAPGAASAAVAS"
     gene            complement(1277..3196)
                     /gene="asnB"
                     /locus_tag="VEC56_02265"
     CDS             complement(1277..3196)
                     /gene="asnB"
                     /locus_tag="VEC56_02265"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV13008.1"
                     /translation="MCGIAGFMSRNGDSAQLARDLARAVACLRHRGPDDEGLWNDGAG
                     TGLGFRRLSILDLSERGHQPMRSKDGRYVMVFNGEVYNFAEVRARLLGLGHLFSGTGD
                     SEVILTAFQQWGERAVDHFIGMFAIGLWDEREQTMHLFRDRVGVKPLYYGWDGTTLWF
                     GSELKALRAFGHWRPRVDRQAVGEFLHYGYISSPRSIYTQVKKLPPGSRLALSRTGDP
                     VVTRYWSLLDALGSPIAGDDDAIEAELEALLASAFQYRMVADVPVGVFLSGGVDSSLV
                     TAILAREATQPIRTFTIGFAEDHRDESAWARKVANHLGTRHTEYILGVQEALAMTRDW
                     GTLFDEPFGDASGIPTLLVSRLARAEVTVALSADGGDELFMGYSVYDDVLGRLDTLAR
                     VPSWLKTASAHSLSHTPVGVLDGALSRLGSSAAVRGRVSRRIRRARAIMHDATPDRVY
                     DAAISYWLPEEVRGLMGDYSNPRTLADAYRGEPLERMCLWDFHHYLPDDIMTKVDRTT
                     MAVSLEGREPMLDHRLAEFAFRLPSRLRRGPLGPKHILKKILYRHVPRELVDRPKQGF
                     AIPLEGWLRDDLRALAGDYLAEARIRNAGYFDARVVRRLVDDFYRGNGSTADQLWFLL
                     AFELWREKWGAGVDA"
     gene            complement(3219..>3596)
                     /locus_tag="VEC56_02270"
     CDS             complement(3219..>3596)
                     /locus_tag="VEC56_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13009.1"
                     /translation="HDEVGHVLRRSALLINTSSVEGFPNAYLEAWNHGVPVVAFNDVD
                     GIIREAGVGTVCSSIDDMADAVRKLTADPAAMDAMRNRARRLVRDRFSSQSLGPQYAE
                     FFEELLTARHGAGAPPSVSVRRP"
BASE COUNT          641 a         1248 c         1139 g          568 t
ORIGIN      
        1 gtccatgaag cgttcgaagc gcgcccccat gcgctcgggg ccgggaccga agcgcgtcgc
       61 gatctgcgcg ctgcggcgca cgaggttgcc cgcgccgcgg tgaccgaata cgaacttgag
      121 gacgttcatg acgccaccgc cgcgctggcc gcccccggcg cgcgcgtgag caactcgtcg
      181 aagagcgctt ccctcgccgc gcacatcgcc tcgatgcgaa agcgttccat ggcgatttcg
      241 cgcccgcgat gaccgaaggc ccgccgccgg tcggggctgc taacgagctc ggcgagtgcc
      301 gctgcgagtg cgtcgtcgtc gccgggcgga accacgaggg cgctcatccc cggctcgacc
      361 atctcgcgga cactcccgac gtcggtggcc acgaccggca ggcccgccga catcgcctcc
      421 aacaccacgt ttggaaacgc ctctgtgcgc gacgaaagaa cgaacaggtc cgccgcgcgc
      481 aacagccggt cgacgtcgtc gcggagcccc gcgaagatgg tccgttcggc gattcccaac
      541 tgtcgcgcca gcgactccag cgcgctccgc tccggcccct cgcccacgag cagcacgcgc
      601 accggtgcgc cggcaccgtg cagtcgcgcg gcggccgcga gcagcgcgtc gatgcgcttg
      661 agcgccttca gcgacgccac cgtgacgaga acgacgtcgc gatcgtcgat gccgagctcg
      721 cgccggccgc tggcgcgctc ttcggtcgtc gccggccgga acgtcgacgt gtccacgccg
      781 ttgtggatca cgcgaatgtg ctcgcgcgga agcccttcgt cccggaccaa atagtccttc
      841 tgcacttcgg ccacggccag cagcgcgtcc gcgcgtcgca acagcgggcg caggtacggc
      901 agcacctggc gcgtgccgcc gatgtcgccc atcgcgtggt aggacaccac cacgcgatcc
      961 acgagtccgc ggagcttcgc gaggtaagag aaaatgacgg tgttcgggtg cgagaacgtg
     1021 tagatgagcc cggggcgctc gatcctcagc acgcgctcga ggcccaagaa cgcgaaggcg
     1081 tcgaaacggt gccgctggag cccgtcgtag aacgggacgc ccattgcctc cgtcttgctc
     1141 ttgaagtatc cgccctcctt gagacagcac accgcgaagt gaaatcggct ccggtcggcg
     1201 cgcgcgacga actgaaacaa cgatttctcc ttgccgccca tctcgaactc gttatccaga
     1261 atcaaaattc gatgcatcat gcgtcgacgc ccgctcccca cttctcgcgc cacaactcga
     1321 atgcgagcag gaaccacaac tggtcggcgg tgctgccgtt tccccgatag aaatcgtcca
     1381 ccagtcgtcg aaccacccgg gcgtcgaagt accccgcatt cctgatacgc gcttcggcca
     1441 ggtagtctcc cgcgagcgcg cggaggtcgt cgcgcagcca gccttccagc ggaatcgcga
     1501 acccttgctt gggccggtcc acgagctctc gaggaacgtg gcggtacaat atcttcttga
     1561 ggatgtgctt gggaccgagc ggcccacgcc gcagacggct ggggagccga aacgcgaact
     1621 cggcaagtcg gtggtcgagc atcggctcgc gcccttccag actcaccgcc atggtcgtgc
     1681 ggtccacctt ggtcatgatg tcgtcgggga gatagtggtg gaaatcccag agacacatgc
     1741 gctcgagcgg ctctccgcga tacgcgtccg ccagcgtgcg cgggttcgag tagtctccca
     1801 tcagcccgcg cacctcttcg gggagccagt acgagatggc ggcgtcgtac acgcgatcgg
     1861 gcgtggcgtc gtgcatgatc gcgcgcgcgc ggcgaatgcg gcgcgagact cgaccccgga
     1921 ccgcggccga gcttccgagc cgggacagcg cgccgtccag cacgcccacg ggggtatggg
     1981 acagcgagtg cgcggacgcc gtcttcagcc aactcggcac gcgcgcgagc gtatcgagtc
     2041 gtcccagcac gtcgtcgtag acgctgtagc ccatgaacaa ctcgtcgccg ccgtccgcgg
     2101 agagcgccac cgtgacttcg gcccgcgcca gtcttgaaac caacaaggtc ggtattcccg
     2161 acgcgtcccc gaacggctcg tcgaacagag tcccccagtc gcgcgtcatc gcaagcgctt
     2221 cttgcacgcc gagaatgtac tccgtgtgcc gagtaccgag gtggttcgcc actttgcgcg
     2281 cccacgcgga ctcgtctctg tggtcctccg cgaagccgat cgtgaaggtt cgaatgggct
     2341 gcgtcgcttc gcgggccagg atggcggtca ccagcgacga gtcgacgccg cccgagagga
     2401 atacgcccac gggaacgtcg gccaccatcc gatactggaa cgcgctcgcc agcagcgcct
     2461 ccaactcggc ttcaattgcg tcgtcgtcac cggcgatcgg gcttcccagc gcgtcgagca
     2521 gcgaccagta gcgcgtgacg accggatcgc ccgtgcgcga cagcgcgagc ctcgatccgg
     2581 gcggcagctt cttcacctgg gtgtagatgc tgcgcggcga cgagatatac ccatagtgca
     2641 gaaactcgcc taccgcttgt cggtcgacgc gcggacgcca atggccgaag gcgcgcaggg
     2701 ccttgagttc ggagccgaac cacagcgtgg tcccgtccca gccgtagtac aacggcttga
     2761 ccccgacacg atcgcgaaac aggtgcatgg tctgctcgcg ctcgtcccac agaccaatcg
     2821 cgaacatgcc gatgaagtga tcgaccgcgc gttcgcccca ttgctggaag gcggtgagga
     2881 tcacttcgct gtcgccggta cccgagaaca aatgccccaa accgagcagt ctcgcgcgca
     2941 cttcggcgaa gttgtagacc tcaccgttga acaccatgac gtagcgtccg tccttggatc
     3001 gcatgggctg gtgaccgcgt tcgctcaaat cgaggatcga cagcctccga aaccccaggc
     3061 cggtaccggc gccgtcgttc cataggcctt cgtcgtcggg gccgcggtgg cgcaagcacg
     3121 ccaccgctcg cgcgaggtct cgcgcgagct gtgccgagtc gccgttacgc gacataaatc
     3181 ctgcgattcc acacatgggc tagagattcc gcgcggtttc agggacggcg caccgagaca
     3241 cttggcggcg cacccgcgcc gtgacgcgcc gtcaataact cctcgaagaa ctccgcgtac
     3301 tgcgggccga gcgattgcga cgaaaaacgg tctcggacga gtcgccgcgc gcgattgcgc
     3361 atggcgtcca tggccgccgg gtcggcggtc agtttgcgaa ccgcgtccgc catgtcgtct
     3421 attgaagaac acacggtgcc aacgccagcc tcgcgaatga tgccgtcgac atcgttgaac
     3481 gcgaccaccg gcacaccgtg gttccaggct tccagatacg cattgggaaa cccctcgacc
     3541 gacgacgtgt tgatgagtaa cgccgaacgg cgcaggacgt ggcctacttc gtcgtg
//
LOCUS       DASPGK010000083         4844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_840370, whole genome shotgun sequence.
ACCESSION   DASPGK010000083 DASPGK010000000
VERSION     DASPGK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840370"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VEC56_02275"
     CDS             complement(<1..1472)
                     /locus_tag="VEC56_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXV13010.1"
                     /translation="MGRQDQVLCLEGGEAAARVAYRLSEVIAIYPITPSSVMGELSDE
                     WAAHGQPNVFGTVPRVIEMQSEGGAAGAVHGALQAGALTTTFTASQGLLLMIPNMYKI
                     AGELTSTVFHIAARTVATHALSIFGDHSDVMAARQTGFAMLFSASVQEVMDLALVAHV
                     ATLDARVPFLHVFDGFRTSHEIQKIDVLSDEDLRAMLPAISVRAHRERALSPDHPVVR
                     GTAHNPDTYFQAREAANPFYLATPAIVQRTMDALAARTGRHYRLFDYVGAPDAERVIV
                     AMGSACETIEETVATLSARGDKVGVVKVRLFRPFDARALVEALPPSTRVVSVLDRTKE
                     PGGAGEPLYQDVVTALHEDAQSSTARFARVPRVVGGRYGLSSKEFTPAMVKAVLDDAA
                     SAAPKNHFTVGIHDDVTHTSLEYDPAFLLDGRDVVSAVFWGLGSDGTVGANKNSIKII
                     GEQTDRFVQGYFVYDSKKAGARTVSHLRFGPSPIRSPYLI"
     gene            complement(1666..>4844)
                     /locus_tag="VEC56_02280"
     CDS             complement(1666..>4844)
                     /locus_tag="VEC56_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13011.1"
                     /translation="TTTYRDAANTTADWNTAAGELRLFPFVVSELGSYATTGFNLSVA
                     VSGDLAFIGDTGNGLRVLDIEDPANPVLLGTAAPPFACFDIVAHGDLVFVADGAGGLR
                     IVDVTDPTTPTQIGNYATTDARAVAIFGYSVIVADNLGGLVVVDVTNPASPTLVSTYD
                     TPGEALGVTTSGLRVIVADGTAGLHVVDLTNLASPTLLGSYDTVGEARAIAMDGLVAL
                     VADGLSGLHAISTSDPVNPALLGSYDTPGFAQSVFVAGDRAYVADGMGGGLQAIDITN
                     ASALSLILAYDTPGTTHDVVVDGTEAFVADRGSFRVIAVGSLVDAEIGSLGTALYPRV
                     HVSGNLAAVASEAGFDFVDVSNPANPVIASSFSFFEMISQVVIDGSRAYVAAIGSLSR
                     VDITDPSNPTFLPGYAEVTAYDMAVAGGLAYVSGSGGIRILDVSYPDTLVQIGSAANG
                     RDGVFVSGNQVYGWTRQLLQSTLSIYVVDDPTAPVSAALVFFTGQINDVFVAGDRAYI
                     AAGWSNVDPTGSDGLWIYDVADVTNPQLITRFDPPGNANVRSVSVSGNNAIVGIDNAL
                     WFLDVTNPASPVVQEVRTVTGGATSVAFDGEHAFVTDPSNLRVLTVLQSQVIGLDKEA
                     QSLLVDGPASGILRARLTTTQSGSISLEIAADSPATWQLVTPGSTWHRLDVPGDDLTW
                     RSTLNWMPGQIPSISDLQLEWLTAAAIVQTIADVPNDQGGWLRVNFARSGYDFVEETV
                     TPVTNYGIWRRVDDLALVASLDGARQPDADVAIAEAFRGMPLRAVGDRVFVQSSRVTD
                     ADFPAGTWELVMNVPSLQKQTYLAAIPTAADWTPSADNYTALVITAHTTTPSIWFASS
                     PDSAYSVDNIAPSVPSSFVVAYNTGSGNALSWDESPDEDFQYFRVYRSSNPSFTPSAG
                     TLVHETIAAGWSDPDYDGWPVYYKVTALDYAGNESAPSGTGTTTDVGDAAPRTFALYP
                     NVPNPFNPTTLIRYDVPASGGEVTLRIYDVSGRPVKTLVEGVQSAGSKSATWDGRDDA
                     GRAVASGVYFYRLAAPGFTKTHKMVLMK"
BASE COUNT          856 a         1554 c         1616 g          818 t
ORIGIN      
        1 tcgatcaggt acggactccg gatcggcgac ggcccgaagc gcaggtgcga gaccgtgcgc
       61 gcccccgcct ttttcgagtc gtagacgaag tagccctgca cgaagcgatc ggtttgttct
      121 ccgatgatct tgatcgagtt cttgttggcg ccgacggtgc cgtcgctccc aaggccccag
      181 aataccgcgc tcaccacgtc gcgcccgtcg agcaggaacg ccggatcgta ttcgaggctg
      241 gtgtgggtga cgtcgtcgtg gatgccgacg gtgaagtggt tcttcggcgc cgcgctggcc
      301 gcgtcgtcga ggaccgcctt gaccatggcg ggcgtaaact ccttcgacga gagaccgtag
      361 cgcccgccga ccacgcgcgg cacgcgcgcg aagcgcgccg tggaactctg cgcatcctcg
      421 tgcagggcgg tcaccacgtc ctgatagagc ggctcgccgg ccccgcccgg ttccttggtg
      481 cgatcgagca cggagaccac gcgggtcgac ggcgggagcg cttcgaccag cgcgcgcgcg
      541 tcgaagggac gaaagaggcg gactttgacc acgcccacct tgtcgccgcg cgcggagagc
      601 gtagcgacgg tctcctcaat ggtctcgcag gccgagccca tggcgacgat cacgcgttcc
      661 gcgtcgggcg cgcccacgta gtcgaagagg cgataatgcc gccccgtgcg cgccgccagc
      721 gcgtccatcg tgcgctgcac gatggcgggc gtcgcaagat agaacggatt ggccgcctcg
      781 cgcgcctgga agtaggtgtc ggggttgtgc gcggtgccgc gcaccacggg atgatccggt
      841 gacagtgccc gctcgcggtg ggcgcgcacc gagatcgcgg ggagcatcgc gcgcaagtct
      901 tcgtcggaga gcacgtcgat cttctggatc tcgtgcgagg ttcgaaaacc gtcgaagacg
      961 tgcagaaacg gaacgcgtgc gtcgagcgtg gcgacgtgcg cgaccagcgc caggtccatg
     1021 acctcctgca cgctcgcgga gaagagcatc gcgaagccgg tctggcgcgc ggccatgacg
     1081 tcgctgtggt ccccgaagat cgaaagcgca tgggtggcca cggtgcgcgc cgcgatgtgg
     1141 aacaccgtgc tcgtcagctc gcccgcgatc ttgtacatgt tcgggatcat gagaaggagg
     1201 ccctgactcg cggtgaaggt cgtggtcaaa gcgccggctt gcagcgcgcc gtgcaccgcg
     1261 cccgcggcgc ccccctcgct ctgcatctcg atgacgcgcg gtaccgtgcc gaaaacgttc
     1321 ggctgaccgt gcgcggccca ctcgtcggac aattcgccca tgacggagga cggcgtgatg
     1381 ggatagatgg cgatgacttc gctcagtcgg taggcgacgc gcgcggcggc ctcgccgcct
     1441 tcgaggcaca ggacctggtc ctgccgcccg atcgaaaacg acgaggtatt ggcgggggag
     1501 cttttcgacg actggtcgcg cgcggggatg gacccggcgc tcttcgcgcg gcccttggtt
     1561 acattcggca cgtggatgcc tttctataga ctgcccgcaa gcgggcaact cggcgcggcc
     1621 acatgcagtc tacggcattc gggcgcgaac ggcaaggaac gcggtctatt tcatcagcac
     1681 catcttgtgg gtcttcgtga agcccggtgc cgcgaggcgg tagaaataca cgcccgacgc
     1741 caccgcgcgc cccgcgtcgt cgcggccgtc ccacgtcgcc gacttcgacc ccgcggattg
     1801 cacgccctcg accagcgtct tcaccggccg cccgctcacg tcgtagatgc gcagcgtcac
     1861 ctcgcccccg ctggccggca cgtcgtagcg aatgagcgtg gtcggattga acggattggg
     1921 gacgttcggg tagagcgcga acgtccgcgg agcagcgtct cccacatcgg tcgtggtccc
     1981 cgtgccggac ggcgcgctct cgttgcccgc gtagtccagt gcagtcacct tgtaatagac
     2041 cggccagccg tcgtagtccg gatcggacca gccggcggcg atggtctcgt gcaccagggt
     2101 gccggccgac ggcgtgaagc tgggattgct cgaacggtac acgcggaagt actggaagtc
     2161 ctcgtcgggc gattcgtccc acgagagcgc gtttccgctc ccagtgttgt acgcgaccac
     2221 gaagctgctc ggcaccgacg gcgcgatgtt atcgaccgag taggcgctgt ccggcgagct
     2281 cgcgaaccag atcgagggcg tggtggtatg cgcggtgatc acgagcgcgg tgtagttgtc
     2341 cgccgacggc gtccaatcgg ccgcggtcgg aatcgcggcc agataggttt gcttctgcag
     2401 cgaaggcacg ttcatgacca actcccacgt tccggccggg aagtcggcat cggtcacccg
     2461 agacgattgc acgaacacgc ggtcgcccac ggcgcgcaac ggcatcccgc gaaacgcctc
     2521 cgcaatcgca acgtccgcgt ccggctggcg ggccccatcc agcgacgcca ctagcgcaag
     2581 atcgtcgacg cgtcgccaga tgccgtagtt ggtcaccggc gtgacggtct cttcgacgaa
     2641 gtcgtagccg ctgcgcgcga agttgacacg tagccatccg ccctggtcgt tggggacgtc
     2701 ggcgatggtt tggactatgg cggccgctgt cagccattcg agctggaggt cggagatgct
     2761 cggaatctgc cccggcatcc aattgagcgt cgagcgccac gtgagatcat cgccgggaac
     2821 atcgagacgg tgccacgtac tgcccggcgt taccaactgc cacgttgcgg gggagtctgc
     2881 cgcgatctca agcgagatgc tgccgctctg ggtcgtggtc agtcgcgcgc ggaggatccc
     2941 gctcgcgggc ccgtcgacga gcaaggattg cgcctccttg tcgaggccga tgacttgaga
     3001 ttgaagtacc gtcaatactc gcaggttcga cggatccgtc acgaacgcat gctcgccatc
     3061 gaaagcgaca ctcgtggctc cgccggttac cgtccgcacc tcttggacta cgggcgacgc
     3121 gggattcgtc acatcgagaa accacagcgc gttgtcgatg ccaacgatgg cgttgttccc
     3181 cgagacgctg acactcctga cgttggcatt accgggggga tcgaatctcg taatgagttg
     3241 cggattcgtg acgtccgcga cgtcgtaaat ccacagcccg tcgctgccgg tcgggtccac
     3301 gttactccaa ccagcggcta tgtaagcacg atcgccggca acgaagacat cgttgatttg
     3361 acctgtgaag aagactaggg ctgccgagac gggggcggtc gggtcgtcga cgacgtagat
     3421 gctcaaggtg ctctgaagaa gttgtcgagt ccaaccgtac acttggttgc cggaaacgaa
     3481 gaccccgtcc cttccattcg ccgcggagcc gatctgtacc agggtgtcgg ggtacgatac
     3541 gtcgagtatg cggatacccc cagaaccgga tacgtaggca agaccacctg ccaccgccat
     3601 gtcatacgcc gtcacttccg cgtagccggg aaggaaagtc ggattggacg gatccgtgat
     3661 gtccactcga gagagcgaac caatggcggc gacgtacgcg cgactaccgt cgatcacaac
     3721 ctggctgatc atttcgaaga acgaaaacga actcgcgatc accggattgg ctgggttgct
     3781 gacatcgaca aaatcaaacc cggcttccga agcgaccgcc gcgagattgc ccgacacgtg
     3841 gacgcggggg tagagcgctg tgcccaagct cccaatctcg gcgtcgacca gacttccgac
     3901 tgcaatcaca cgaaaacttc cacgatctgc gacgaacgcc tccgttccgt cgaccacgac
     3961 gtcgtgcgtc gtgcccggcg tgtcatacgc gagaatgagc gacagggccg aagcgttggt
     4021 gatgtcgatc gcctgcaggc cgccgcccat tccgtccgcc acgtacgcgc gatcgcccgc
     4081 gacgaagaca ctctgcgcga agcccggagt gtcgtatgag ccgagcagcg cgggattcac
     4141 gggatcgctg gtgctgatcg cgtgcagccc ggagagtcca tccgccacca acgcgacgag
     4201 tccgtccatc gcgatcgcgc gcgcttcgcc gacggtgtcg tacgacccga gtagcgtcgg
     4261 gctcgcaagg ttggtgaggt cgaccacatg gagccccgcg gttccatccg cgacgattac
     4321 gcgcagcccc gacgtcgtca cgccgagcgc ttcgccgggt gtgtcgtacg tactgacgag
     4381 cgttgggctc gcgggattgg ttacgtcgac gacgacgagg ccacccaggt tgtcggcgac
     4441 gatcaccgag tagccgaaga tggcgacggc gcgcgcgtcg gtcgtcgcgt agttgccgat
     4501 ctgcgtcggc gtcgtggggt cggtgacgtc gacgatgcga agcccgccgg cgccgtcggc
     4561 gacgaacacg aggtcgccgt gtgcgacgat gtcgaagcac gcgaacggcg gcgccgccgt
     4621 cccgaggagc accggattgg ccggatcctc gatgtccaac acgcgaagcc cattgccggt
     4681 gtctccgatg aaggcgaggt cacccgacac ggcgacactc aagttgaatc ccgtcgtcgc
     4741 atagctccca agttcggaga cgacgaacgg aaagagccga agctcgcccg cggcggtatt
     4801 ccaatccgcg gtggtgttgg cggcgtctcg ataggtcgtg gtcg
//
LOCUS       DASPGK010000084         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1543144, whole genome shotgun sequence.
ACCESSION   DASPGK010000084 DASPGK010000000
VERSION     DASPGK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543144"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEC56_02285"
     CDS             complement(<1..194)
                     /locus_tag="VEC56_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV13012.1"
                     /translation="MLHVRIISGNSKGRTVALEPANLPLTIGRDPENLVVLDHTAISR
                     FHCRITSENGLYYVQDLGST"
     gene            429..2654
                     /locus_tag="VEC56_02290"
     CDS             429..2654
                     /locus_tag="VEC56_02290"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXV13013.1"
                     /translation="MSTAYDTIEFRPSALRVLQARYLRKDTAGNPAETPDEMFRRVAG
                     AVAAAEAGYGASPEEQRRWEDAFYRSMCSLEFLPNSPTLMNAGTELPQLSACFVLPVE
                     DSMEGIFDAVKHTAIIHKSGGGTGFSFSRVRPKNDRVKSTHGVSSGPVSFMAVFDAAT
                     ETVRQGGKRRGANMAMLRVDHPDILEFIDAKTSSRRLNNFNLSVALTDSFMDAVANDA
                     SFDLVNPRSGEATGSLRARAVFQSIVDRSWRNGEPGVVFIDRMNRDNPTPALGKIEST
                     NPCGEQPLLPYESCNLGSLNLSRFAAGSFGAATVDEPRLRTAVALAVRFLDDVIDVNH
                     YPLPEIDRLTRTNRKIGLGVMGFADLLVSMGIPYDSPRAVAVAEDIMELVNLAAVEAS
                     QELARQRGPFPAFEQSAYAARKLPARRNATVTTVAPTGSISIIAGTSSGVEPLFALAY
                     QRRVLDGDTLTEVHPRFEEVARREGFYSEALIERIARTGHIADDAEVPERWRAVFQTA
                     HEVSPEGHVRIQAAFQRHTENAVSKTVNLARDAAPDDVERVFRLAFELGCKGVTVYRD
                     GSREAQVLSAGPAVDETRELRVPIARPETLEGRTVRMTTGCGNLYVTTNEGERGPLEL
                     FAHIGKAGGCATSLTEAIGRLVSLALRSGVDPFAVARQLRGVRCPFPSWNRGHRVLSC
                     ADAIGQVLEDGLTASPVSSEATAPGGRAERLAGTCAECGSVLEFEGGCVVCRACGFTR
                     C"
     gene            complement(2665..3393)
                     /locus_tag="VEC56_02295"
     CDS             complement(2665..3393)
                     /locus_tag="VEC56_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13014.1"
                     /translation="MAKPTQPETPVVAPERASAHPGWWTSLAVLTLTWLGIPFLAVGA
                     AFPWYLFRRANPELSRGLTPAIRWGLAAWVTAVAMTALAGSRALRAIPFGADAASSAR
                     AWIEGSGAVPSWTAMAIATVVFAAAAAVSPGILGAIALIHVVLITAVHAAVVFSSSSN
                     VLGACVVALPVWSALLLAGMITLLEPLGQWGETTLRRRRGDGAPTVSRTRLMVGAGLI
                     GAAFAARLVLAEPLGDLLRRVTLP"
     gene            3623..4225
                     /locus_tag="VEC56_02300"
     CDS             3623..4225
                     /locus_tag="VEC56_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HXV13015.1"
                     /translation="MKKSVCTALCLVVALAAAAFAGDEKKAAQQPQMDPAAMQAMMTA
                     MSPGEHHEHMKKLAGNHTYTMKMWMDPSAPPQESAGKRTSEFLLGGRFLQEKYSGTFM
                     GMPFEGIGILGYDNVKKEYVGTWIDNMSTGIMTSHGTCDKGSWTMTGESTDPMTGKSW
                     TSRSVVKLVDDNTFVMEMYAPGPDGKEFKMMEMTCKKAAM"
     gene            complement(4289..>5854)
                     /locus_tag="VEC56_02305"
     CDS             complement(4289..>5854)
                     /locus_tag="VEC56_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HXV13016.1"
                     /translation="SRPDACAERVFAPWIDMEAELRRRELPLVTLETFTPLDRFDVIG
                     FSMQYELGFTNLLNMLELGRVPVLTKDRSISDPVVLGGGSITLAMEPVAPFFDAILVG
                     DAEDVIHPVLDAVKAWRRSGRPRADLHRALCAIPGMYVPSLYHIDYNADGTIARIDHV
                     PPAPANIPKTTVWDLENAPFPVRPVIPNVEVIHDRINIEIMRGCPNQCRFCQAVQHYR
                     PLKMRSIAKVMELAEAAYKETGYDEISLTSLSTADYPGIVDLLRAVSERFRARRVNVS
                     LPSLRVGPGLRELPGLTSTVRKSGLTMAPEVATDRLREVIRKPIKNEDLMTGCAAAYD
                     AGYRLVKFYFLIGAPTETDDDLRGIVELAEAASRLRLQSHGRRATINCSISSHIPKPH
                     TPFQWEEMNSREELVAKQRYLKSLNRSSQIALKFHDVDISYLEAVFSRGDRRLATSLL
                     KAREAGLRMDAWSECFDIRKWGDVFRDAGVDPDWYALRARSTDEILPWDMIGLKIPTS
                     HLAKEHARAHAAA"
BASE COUNT         1042 a         1942 c         1957 g          913 t
ORIGIN      
        1 ttggtgctgc ccaggtcctg gacgtagtag agcccattct cgctggtgat ccggcagtga
       61 aagcgggaga tcgcggtgtg gtcgagcacc acgaggttct cggggtcgcg accgatggtg
      121 agcggcaagt tggccggctc gagtgccacg gtcctaccct tcgagtttcc ggagatgatg
      181 cgtacgtgca gcatggggta ccaggccgcg acacgggcgg caaccccaag aaagcaagaa
      241 tcaggtcaaa cgaagcaccg cctcccgcgt cggcgcggag aggccactct tcctcgtaac
      301 ctattgttta tacgtctgtt ggcatcatcc gaccacggag cccgaaagtt tattgaagat
      361 aatcacgtct taatatatga atactgcgag cagctttttg ccgcggtccg caaaccaaag
      421 gggcgatcat gagcacggca tacgacacca tcgagttccg cccgagcgcg cttcgcgtgc
      481 tccaggcgcg ctacctccgc aaagacacgg ccggaaatcc ggccgagacg cccgacgaaa
      541 tgttccggcg cgtggccggt gccgtggccg cggcggaggc cggctacggc gcgtctcccg
      601 aagagcagcg gcgctgggag gacgcgttct accgcagtat gtgctcgctc gagttcctgc
      661 ccaattcgcc gaccctgatg aatgccggca ccgagctccc ccagctctcc gcgtgcttcg
      721 tgctcccggt cgaggactcc atggaaggca tcttcgacgc cgtcaagcac accgccatca
      781 ttcacaagag cgggggcggc accggtttct cgttttcgcg cgtgcgcccg aagaacgacc
      841 gcgtcaagag cacgcacggg gtttcgagcg ggccggtctc gttcatggcc gtgttcgacg
      901 ccgcgaccga aaccgtgcgg caaggcggca agcggcgcgg cgccaacatg gcgatgctgc
      961 gggtcgacca cccggacatc ctcgagttca tcgacgccaa gacgtcgagc cggcggctca
     1021 acaacttcaa tctgtcggtg gcgctcacgg attcgttcat ggatgcggtc gcgaacgacg
     1081 cctcgttcga cctcgtcaac ccgagaagcg gtgaagcgac cggatccctg cgcgctcgcg
     1141 ccgtgttcca atcgatcgtc gaccgctcgt ggcgcaacgg cgagccggga gtggtcttca
     1201 tcgaccgcat gaaccgcgac aatcccaccc ccgcgctggg caagatcgaa agcaccaacc
     1261 cgtgcggcga acagccgctg ctcccctacg agtcgtgcaa cctcggatcg ctgaacctgt
     1321 cccgattcgc ggccggctcg ttcggggccg ccaccgtcga cgaaccgcgc ctgcgcacgg
     1381 cggtggccct cgcggtccgc ttcctcgacg acgtgatcga cgtcaatcac tacccgctgc
     1441 cggagatcga ccggctcacc cgcaccaacc gcaagatcgg gctcggcgtg atgggcttcg
     1501 cagacctgct ggtgtcgatg gggattccgt acgattcccc gcgcgcggtg gcggtcgccg
     1561 aggacatcat ggagctcgtg aacctcgcgg ccgtggaagc gtcgcaggag ttggcgcgac
     1621 agcgcggccc gttcccggcc ttcgagcaga gcgcatatgc cgcgcgcaag ctgccggcgc
     1681 ggcgaaacgc gaccgtgacc acggtggcgc cgaccggatc gatcagcatc atcgccggaa
     1741 cgtcgagcgg agtggagccg ctgttcgcgc tcgcgtacca gcggcgcgtc ctcgacggcg
     1801 acacgctgac cgaggtgcat ccgcgcttcg aggaagtggc gcgtcgtgag ggcttctact
     1861 ccgaggccct gatcgagcgc atcgcgcgca ccgggcacat cgccgacgac gccgaggtgc
     1921 ccgagcgctg gcgcgcggtc ttccagaccg cgcacgaggt atccccggaa gggcacgtgc
     1981 gcatccaagc cgctttccag cgccacaccg agaacgcggt gtcgaagacc gtgaatctcg
     2041 cgcgcgacgc cgctcccgac gacgtcgagc gcgtgtttcg gctcgccttc gagctgggct
     2101 gcaagggtgt taccgtgtat cgcgacggga gccgggaggc gcaggtcttg agcgccgggc
     2161 cggccgtgga cgaaacgcgg gagttgcgcg tgccgatcgc gcgacccgaa acgctcgagg
     2221 gccgaaccgt gcgtatgacg accggctgcg gcaacttgta cgtcaccacc aacgagggcg
     2281 agcgcggtcc gctcgagttg ttcgcgcaca tcggcaaggc gggcgggtgc gcaacctctc
     2341 tcaccgaggc gatcggccga ttggtgtcgc tcgcgctgcg ctccggcgtc gaccccttcg
     2401 ctgtggcgcg ccagttgcgc ggcgtgcgct gcccgtttcc gtcctggaac cgtggccacc
     2461 gcgtcctctc gtgcgccgac gcgatcggac aagtgctcga agacggcctg accgcatcgc
     2521 ccgtctcgtc cgaagcgacg gcccccggtg ggcgcgcgga gcgtctggcc ggtacctgcg
     2581 ccgagtgcgg gagcgtgctc gagttcgaag gcgggtgtgt cgtgtgccgc gcgtgcggat
     2641 tcacgcgctg ctagcgcgat cgcgctaggg aagcgtcaca cgtcgaagga ggtcgccgag
     2701 cggttcggcc aggaccaggc gcgcggcgaa ggcagcgccg atcaatcccg ctcccaccat
     2761 gagacgcgtg cgtgaaacgg tcggagcccc atcgccgcgc cggcgtcgca gcgtggtttc
     2821 gccccactgt ccgagcggtt ccaagagagt gatcattccc gccaagagca gcgccgacca
     2881 gaccggcagt gcgaccacgc acgcgccgag cacgttcgac gaactcgaga atacgaccgc
     2941 ggcatgaacg gcggttatca agacgacatg gatcaacgcg atggcgccca ggatgccggg
     3001 cgagacggcc gccgccgccg cgaacacgac cgtcgcgatc gccatcgccg tccacgacgg
     3061 caccgcgccg cttccctcga tccacgcccg cgccgaactc gccgcatcgg cgccgaacgg
     3121 aatcgcgcgc agcgcgcgac tgcccgccag cgccgtcatc gcgaccgccg tcacccacgc
     3181 ggcgagtccc catcgaatcg cgggcgtcag ccctctcgat aactccgggt tggcccttcg
     3241 aaagaggtac caggggaacg cggctccgac ggcgaggaag ggaatcccca gccaggtaag
     3301 ggtcaatacc gccaacgagg tccaccagcc gggatgggcc gaggcgcgct cgggggcgac
     3361 gacgggggtc tcgggttggg tcggtttggc catgccatac ccgccaggaa ggcgggcccc
     3421 gtaggctacc acagcccggc gaccgcgcaa agccaagccc cttgagggcg cccgcggcgt
     3481 atgatatcgt gcccttggct ttgaatccca ccgcgcgtgc gccccaggac cgcgtcggaa
     3541 aacgccctgc ctcgagcggg gcacgggccg ccacgatggc ggcgctacaa cgtccaacga
     3601 cacgataggg aaaggtgagc ttatgaagaa gtccgtgtgt accgcactgt gcttggtcgt
     3661 cgctctggcg gcggccgcgt tcgccggcga cgagaagaag gccgcgcagc agccccagat
     3721 ggatcccgcc gccatgcagg ccatgatgac cgcgatgtcg ccgggcgaac accacgaaca
     3781 catgaagaag ctggccggca accacacgta cacgatgaag atgtggatgg atccttcggc
     3841 acccccgcag gaatcggccg gcaagcgcac ctcggagttc ctgctcggcg gtcgtttcct
     3901 ccaggagaaa tactcgggca ccttcatggg catgcccttc gaaggcatcg gcatcctggg
     3961 ctacgacaac gtgaagaagg agtacgtcgg cacgtggatc gacaacatga gcaccggcat
     4021 catgacctcg cacggcacct gcgacaaggg tagctggacc atgacgggcg agagcaccga
     4081 ccccatgacc ggcaagtcgt ggaccagccg cagcgtggtc aagctcgtgg acgacaacac
     4141 gttcgtgatg gagatgtacg cgccgggtcc ggacggcaag gaattcaaga tgatggagat
     4201 gacctgcaag aaagcggcga tgtagcccaa caggtcgatt cgcatcgatt cgcgaaaggg
     4261 agtcgccgtt cggcggctcc ctttttcgtt aggccgcggc gtgcgcgcgc gcgtgctcct
     4321 tggccagatg cgaggtggga atcttgaggc cgatcatgtc ccagggcagg atttcgtcgg
     4381 tggaacgggc gcgcagcgcg taccagtccg ggtcgacccc ggcgtcgcgg aacacgtcgc
     4441 cccacttgcg aatgtcgaaa cactccgacc acgcgtccat gcgcaggccg gcttcgcgcg
     4501 ccttcaacaa ggacgtcgcc aggcggcgat cgccgcgcga gaataccgcc tccaggtaac
     4561 tgatgtcgac gtcgtgaaac ttgagcgcga tctgcgacga tcgattgagc gacttcaagt
     4621 agcgctgctt cgcgacgagc tcctcgcggc tgttcatctc ctcccactgg aagggcgtgt
     4681 gcggcttcgg aatgtgggac gagatcgagc agttgatggt cgcgcggcgg ccgtgcgact
     4741 gcagccgcag ccggctggcg gcttcggcca gctccacgat tccgcgcaag tcgtcgtcgg
     4801 tctcggtggg agcaccgatc agaaaataga acttcaccag gcggtagccc gcgtcgtacg
     4861 cggccgcgca ccccgtcatc aggtcctcgt tcttgatcgg cttgcggatc acttcgcgca
     4921 agcggtcggt cgccacttcg ggggccatgg tcagccccga cttgcgcacc gtgctcgtca
     4981 gccccggcag ctcgcgcaac cccggcccca cgcgcagcga ggggagcgac acgttcacgc
     5041 gccgcgcgcg gaatcgttcc gacaccgcgc gcaagagatc gacgatgccg gggtagtcgg
     5101 cggtcgacag gctggtcagg ctgatttcgt cgtagccggt ctccttgtag gccgcctcgg
     5161 ccagttccat cactttcgcg atcgagcgca tcttgagcgg gcgatagtgc tgcacggcct
     5221 ggcagaagcg gcactgattc gggcacccgc gcatgatctc gatgttgatg cggtcgtgga
     5281 tcacttcgac gttggggatg accggacgca ccggaaaggg cgcgttctcg aggtcccata
     5341 ccgtggtctt cggaatgttg gccggcgccg gcgggacgtg gtcgatgcgc gcgatggtgc
     5401 cgtcggcgtt gtagtcgatg tgatagagcg acggcacgta catgccgggg atcgcgcaca
     5461 gcgcgcgatg caggtcggcg cgcggccggc cgcttcgtcg ccacgctttg acggcgtcga
     5521 gcacgggatg aatgacgtct tcggcgtcgc ccaccaagat ggcgtcgaaa aagggagcca
     5581 ccggctccat cgcgagggta atcgacccac cgcccagaac caccgggtcg gagatgctgc
     5641 gatctttggt caggaccggc acgcggccca gctcgagcat gttgagcagg ttggtgaacc
     5701 ccagctcgta ctgcatcgaa aacccgatca cgtcgaagcg atcgagcggc gtgaacgtct
     5761 cgagcgtcac caagggcagc tcacggcggc gcaactcggc ctccatgtcg atccagggcg
     5821 cgaacacgcg ctcggcgcac gcgtcggggc ggct
//
LOCUS       DASPGK010000085         5473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1309524, whole genome shotgun sequence.
ACCESSION   DASPGK010000085 DASPGK010000000
VERSION     DASPGK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309524"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1103)
                     /locus_tag="VEC56_02310"
     CDS             complement(90..1103)
                     /locus_tag="VEC56_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HXV13017.1"
                     /translation="MNRTRVLVACILCLAPSAARAAEVASTVVTRPVHTYSIVARDSV
                     TGEMGVAVQSHWFSVGTVVSWAEAGVGAVATQSLSEITYGPLGLDLMRAGKTAPQALA
                     ALLASDPQREVRQVAMVDAHGNVAVHTGSRCIAEAGHRRGRQFSVQANLMEKDTVWNA
                     MARAYERTHGDLAAKLLAALDAAQAEGGDIRGTQSAAILSVPGTSTGRPWADRVVDLR
                     VDDHADPVREIRRLVDVHRAYQHMNEGDEKLALNDVDGAMSEYAAAAKMLPNNVEVKY
                     WAAITMITAGREKEAMPYFREVFSKDRNWVEVTKRLPASGLLPDDPGLIEKILGAAPK
                     KKR"
     gene            1238..2506
                     /gene="pepT"
                     /locus_tag="VEC56_02315"
     CDS             1238..2506
                     /gene="pepT"
                     /locus_tag="VEC56_02315"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HXV13018.1"
                     /translation="MRPPKEPLLDRFLRYVKIDTRSDENSDSCPSTAKQWDLLRLLHE
                     ELLGLGLKDATIDTNGYVFATVPSNLPPEHAAQVPIVGFIAHVDTAPSITGTNVKPVV
                     HRNYRGGDIALPGDPAQIIRAAEYPNLEKMYVGMDIVTSDGTTLLGADDKAGVAEIMT
                     AVDLFLQNPDIPRGIVKVGFTPDEEIGRGADRFDVARFEAKVAYTMDGGPLGEIEYET
                     FNAFSATITFDGFGVHPGYAKNKLVNAIRMVGDFITRLPRELSPEATESREGYLHPYV
                     INGQSETVTLRMLLRDFEMAGIEKQKAIVKAVAEEVQRLYPKGKVTVDVKESYRNMRY
                     QVEKEPRLVAYAEEAIKRIGETPRHTIIRGGTDGARLSYMGLPCPNLFAGGEAIHSTL
                     EWVPVQVMDKAAETIVRLAEIWAERSATKQ"
     gene            complement(2519..3127)
                     /locus_tag="VEC56_02320"
     CDS             complement(2519..3127)
                     /locus_tag="VEC56_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13019.1"
                     /translation="MTRMLWAVLVSGSVLGLSGSTFAQSNNFLRFVSEPGGSTCSLNY
                     SEPGVVMIHVILDGTAPSSAVQFSAVTPECWTGATWLCDRVDLPFLSLGNTQDPVNGI
                     SVAVNGCKEMPVYIGTIHHQVTDLSASCCAYAPGPAEVQTPAGPLNILATVDCTIPYF
                     MEWVIRPVEPVGITVNAGASCPCELALSTSETTWGRVKALYR"
     gene            3271..3561
                     /locus_tag="VEC56_02325"
     CDS             3271..3561
                     /locus_tag="VEC56_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HXV13020.1"
                     /translation="MCRNIKTLFNFEPPATDEEIRASSLQFVRKLSGTTKPSRANEAA
                     FNRAVEEVAAAARRLIDSMVTSAEPRNRDEEMAKLKARAAERFGRPKSAIER"
     gene            complement(3614..>5473)
                     /locus_tag="VEC56_02330"
     CDS             complement(3614..>5473)
                     /locus_tag="VEC56_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13021.1"
                     /translation="GDLDDGDFFGAGLSSIGDLDGDGVLDLAIGGVFDDDGGFDRGAV
                     WIVFRNADNTVKGHQKISNTEGGFTGDLADSDRFGVSLSSVGTSCTGERVLAVGATLD
                     DDGGFDQGAVWLLRLRADGTVASHSKISDTEGGFTGNLGWEDYFGLGVTSLGDFDGDG
                     AADLAVGARLDDDGGENHGAVYILLLDDCLVQPASIDFGSVTVGQFADASFTVKNSGC
                     DPISGDVSEACDGFSILSGGGPFTIDGGDSIVVSVRFAPTIPGPLDCVVQTGNSGCGS
                     VALTGLGQAAPVDPNPDILSILDVGNDQGRNVRISFARSGRDVLNSPTPILQYEIFRR
                     IDELPSATSGVAAAEKRRDEMVSDRGVLLEGWEYVGSLPAHGETIYNVIAPTLADATH
                     RFGMHWSVFFVRAATAQPLVFFDSPPDSGYSEDNNPPGPPSSLVVAYQPALGNELDWT
                     APSDADVYEYRVYRDDQPAFEPSAESLVHVTSATEWFDAVEMPAQYHYLVAAVDWAGN
                     EGPVVEPSVTTSIGDGVPGQTALHQNVPNPFNPSTTIAYDVAAPGGRVRLEVFDVRGQ
                     LVRTLVDAQQAPGSRSAHWDGRDDRGQRVVSGMYFYRLRFGDLVLTRKMTIVQ"
BASE COUNT         1010 a         1892 c         1704 g          867 t
ORIGIN      
        1 ggccgtgatc gcgttgcggc gcgccttgag cgcgatcgac ttcgtcgagt gcgaagagat
       61 cgacctgcac gccgcctagt gcgttcttcc taacgcttct tcttcggggc ggcacccagg
      121 atcttttcga tcagcccggg gtcgtccgga agcagccccg acgcgggcag gcgcttcgtc
      181 acttccaccc agttgcgatc cttcgaaaac acctctctga agtacggcat cgcttctttc
      241 tcgcgccccg cagtgatcat cgtgatcgcg gcccagtact tcacctcgac gttgttgggc
      301 agcatcttgg cggcggccgc gtattccgac atggcgccgt cgacgtcgtt caaggccagc
      361 ttctcgtcgc cttcgttcat gtgctggtag gcgcggtgca catcgaccaa acgccggatc
      421 tcgcgcaccg ggtcggcgtg gtcgtcgacc cgcaggtcca cgacacggtc cgcccacggc
      481 cgccccgtcg acgtgcccgg cacgctcagg atggcggccg actgcgtgcc gcggatgtcg
      541 ccgccttcgg cttgggcggc gtcgagcgcg gccagcagct tggcggccag gtcgccgtgc
      601 gtgcgctcgt acgcgcgcgc catcgcattc cacaccgtgt ccttctccat gagattcgcc
      661 tgcaccgaga actgccgccc gcgacggtgg ccggcttccg cgatgcaacg cgacccggtg
      721 tgcaccgcca cgttgccatg cgcgtccacc atcgctacct gacggacctc gcgctgcggg
      781 tcgctcgcga ggagcgccgc cagcgcttgc ggcgcggtct tgcccgcgcg catgagatcg
      841 aggcccagcg ggccgtaggt gatctcggag agcgactggg tcgcgaccgc ccccacgccc
      901 gcctccgccc acgacaccac cgtgccgacc gagaaccagt gcgactgcac ggccacaccc
      961 atttcgcccg tcaccgagtc gcgcgcgacg atcgagtagg tgtggacggg acgcgtgacc
     1021 acggtcgacg cgacctcggc cgcgcgcgcc gcggacggcg ccaggcacag aatgcacgca
     1081 acgagcaccc gggttcgatt catggcgact ccttctctgg cgggtagttg acgtcgagga
     1141 gcgaaacgcc tatcctcgca acggtttcac gctaccacga cccggcggaa accgggaagt
     1201 ccacacctgc atcgaactcc aaccacggac ggcgctcatg aggcccccga aagaaccgtt
     1261 actcgaccgc ttccttcgct acgtcaagat cgacacgcgc tcggacgaga attccgactc
     1321 gtgcccgagc acggccaagc agtgggacct gctgcggctg ttgcacgagg agctcctggg
     1381 gctcgggctg aaggacgcca ccatcgacac caacggctac gtcttcgcca ccgtgccgtc
     1441 caacctgccc cccgagcacg cggcccaggt gcccatcgtc ggcttcatcg cccacgtcga
     1501 tacggcgccc tcgatcaccg gcaccaacgt caagccggtc gtgcaccgca actaccgcgg
     1561 cggggacatc gcgcttccgg gtgatcccgc ccagatcatc cgcgcggccg agtatcccaa
     1621 cctggagaag atgtacgtcg gcatggacat cgtgaccagc gacggcacaa cgctcttggg
     1681 tgcggacgac aaggccggtg tggccgagat catgaccgcg gtcgacttgt tcctgcagaa
     1741 tccggacatc ccgcgcggca tcgtcaaggt gggtttcacc cccgacgaag aaatcgggcg
     1801 cggcgcggat cgcttcgacg tggcgcgctt cgaggccaag gtggcctaca ccatggacgg
     1861 cgggcccttg ggcgagatcg agtacgagac cttcaacgcg ttcagcgcca ccatcacgtt
     1921 cgacggcttc ggcgtgcacc cgggctacgc gaagaacaag ctcgtcaacg cgattcgcat
     1981 ggtgggcgat ttcatcacgc ggctcccgcg cgagctgagc cccgaggcca cagagagccg
     2041 cgagggttac ctgcacccct acgtgatcaa cggccagagc gaaaccgtga cgctgcgcat
     2101 gctgctgcgc gacttcgaga tggccgggat cgaaaaacag aaggcgatcg tcaaggcggt
     2161 cgcggaggaa gtgcagcgcc tctatcccaa ggggaaggtc acggtcgacg tcaaggagtc
     2221 gtaccgcaac atgcgctacc aagtcgagaa ggagccgcgc ctggtcgcgt atgccgagga
     2281 agcgatcaag cgcatcggcg agacgccacg gcacaccatc attcgcggcg gcaccgacgg
     2341 cgcgcggctg tcgtatatgg ggctcccctg ccccaacttg ttcgcgggcg gtgaagccat
     2401 ccactcgaca ctcgagtggg taccggttca agtcatggac aaagcggccg aaacgatcgt
     2461 gcgcctcgcg gagatctggg ccgagcgctc ggcgaccaag cagtaatcgc tcaccggctc
     2521 atcgatacag cgccttgact ctcccccagg tggtctcgga agtcgacagc gccaactcgc
     2581 acggacagga agcgcccgcg ttcaccgtga tcccgaccgg ctccaccggc cgaatgaccc
     2641 actccatgaa gtacgggatc gtgcaatcca ccgtggccag tatgttgagc ggtcccgccg
     2701 gggtctggac ctccgccggc ccgggcgcgt atgcgcagca gctcgcggac aagtccgtga
     2761 cctggtggtg gatcgtcccg atgtacaccg gcatctcttt gcagccgttg acggcgaccg
     2821 agattccgtt gacgggatcc tgggtattcc cgagcgacag gaagggcaag tcgacccggt
     2881 cgcacaacca cgtggcgccg gtccagcact cgggtgtgac cgcgctgaac tggacggcac
     2941 tcgaaggagc cgtgccgtcg aggatcacgt gaatcatcac gacccccggc tccgaatagt
     3001 tcaacgaaca cgtcgaacca cccggctcgg acacgaagcg aaggaagttg ttcgactggg
     3061 cgaatgtgga tccagaaagg cccaagacgg aaccggacac caagacggcc catagcatgc
     3121 gagtcatagg aggtcctccc taacgacggg gcgttggacg gaattgtggg gcttgcaccg
     3181 tatgttgtgc gataaactgg agtcggccgg ctagcagtcc tcccccatga aattcgttcg
     3241 atcacggtca gtaccgtcta ggagcctcaa atgtgccgta acatcaagac tctcttcaac
     3301 ttcgagcccc cggcgaccga cgaggagatc cgcgcctcgt ccctccagtt cgtccgcaag
     3361 ctctccggaa ccaccaagcc ctcgcgcgcc aacgaggcgg ccttcaaccg cgcggtcgaa
     3421 gaagtggccg cggcggcgcg gcgcttgatc gattccatgg tgacgagcgc ggagccgcgg
     3481 aatcgtgacg aggaaatggc caagttgaag gcgcgggcgg ccgaacgctt cggccgcccg
     3541 aaatccgcca tcgagcggtg acaacggcgc acgcaacgcc gccttctgct cgcgagccct
     3601 cgctctcccg ccgctactga acgatcgtca tcttgcgcgt aagcacaagg tcgccgaacc
     3661 gcagtcgata gaagtacatc cccgacacga cgcgttgccc gcggtcgtcg cgcccatccc
     3721 aatgggcgct gcgcgatccc ggcgcctgtt gcgcgtcgac cagtgtgcga accagctgcc
     3781 cgcgcacgtc gaagacttcc agccgcacgc gcccgcccgg cgcggccaca tcgtacgcga
     3841 tcgtggtcga cggattgaac gggttgggca cgttctggtg caacgcggtc tgccccggca
     3901 cgccgtctcc gatacttgtc gtcacgctgg gctcgacgac ggggccttcg ttgcccgccc
     3961 agtcaacggc cgcgaccagg tagtgatact gcgccggcat ctcgacggcg tcgaaccact
     4021 cggtcgcact cgtaacgtgc acgagcgact cggccgaggg ctcgaacgcg ggctggtcgt
     4081 cgcgatatac acgatactcg tacacgtccg catcggacgg cgcggtccag tcgagttcat
     4141 ttcccagcgc cggctggtac gcgaccacca agctggacgg cggccccggc ggattgttgt
     4201 cctccgagta gccggaatcg ggcggtgaat cgaagaagac cagcggctgc gcggtggccg
     4261 cacgcacgaa gaacaccgac cagtgcatgc cgaagcgatg ggtcgcatcg gccagcgtcg
     4321 gagcgatgac gttgtaaatc gtctcgccgt gcgcgggcag cgaccccacg tactcccaac
     4381 cctccaacag cacgccccgg tccgaaacca tctcgtcgcg ccgcttctcc gcggccgcca
     4441 cccccgatgt cgcggacggg agctcgtcga tgcgccggaa aatttcatac tgcaggatcg
     4501 gggtcggcga attcagcaca tcccgccccg agcgagcgaa actgatgcgc acgttgcgtc
     4561 cctggtcgtt gccgacgtcg agaatggaga gaatatcggg gtttggatcg accggcgccg
     4621 cctgcccgag gccggtcaga gcgacgctcc cgcaaccgct gttgccggtc tgtacgacgc
     4681 agtcaagcgg gcccggaatc gtgggcgcga atcgaaccga gacgacgatg gagtcgccgc
     4741 cgtcgatcgt gaacggtccg ccgcccgaaa gaatggagaa accatcgcac gcctcgctca
     4801 cgtccccgga aatggggtcg cacccggagt tcttgaccgt gaaggaagcg tccgcgaact
     4861 ggcctaccgt gaccgatccg aagtcgatcg aggcgggttg aaccaggcaa tcatcgagca
     4921 gcaggatgta gaccgcgccg tggttctcgc cgccgtcgtc gtccagccgg gcaccaaccg
     4981 cgaggtcggc ggcgccgtcg ccgtcgaaat ctccgagcga agtaacgcca aggccaaagt
     5041 agtcctccca gcctagattg ccggtgaacc caccctcggt gtcgctgatt ttcgaatgcg
     5101 acgcgaccgt tccatccgcg cgcaagcgca gcaaccacac cgcgccctgg tcgaagcccc
     5161 cgtcgtcgtc gagcgtcgcc cctaccgcga ggacgcgctc tccggtgcag cttgttccga
     5221 ccgacgaaag cgacacgccg aaccggtcgc tgtccgcgag atcgccggtg aacccccctt
     5281 cggtgttgct gatcttctgg tgacccttga cggtgttgtc cgcgttccgg aacacgatcc
     5341 acaccgcgcc gcgatcgaac ccgccgtcgt cgtcgaacac gccgccgatc gctagatcca
     5401 acacgccgtc accgtccaag tcaccgatcg acgaaagtcc ggcgccgaag aagtcaccgt
     5461 catcgagatc gcc
//
LOCUS       DASPGK010000086        14376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1544134, whole genome shotgun sequence.
ACCESSION   DASPGK010000086 DASPGK010000000
VERSION     DASPGK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544134"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6093)
                     /gene="sprA"
                     /locus_tag="VEC56_02335"
     CDS             complement(<1..6093)
                     /gene="sprA"
                     /locus_tag="VEC56_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HXV13022.1"
                     /translation="MALAFVSLLAPVNTARAQGFAAYSIDLGEVGKLYDTLYRDRVTD
                     EIADSVVVYDYHRIRFGGLRYQVPHFLTSDVSTTRAVDDYLAVPGYRARITVPRGAFF
                     EATERQAGDLILTEPRDYTIPDLAVDLRPMDDVATEIYRNTTRQVWRESARASVAALA
                     VTTEATTGGITFDIPLPMPKQLESIFGPGEKTSITLRGREEITIAGETTVLDPFIGAE
                     GRQEQSLFPSLDMQQQLDVSLTGTIGDKVSIQVDHSSEAIGDDANRVRLAYTGYEDEV
                     IQLIELGNTSLSLPGSQLVSVSTNAQGLFGAKMLAKIGSTDFTMIASKQEGEVSSSTF
                     TPTGGALGETEFREIRDVDYVHNKYFYFDNPRAFIGPQAATIEVYKGFINNNTGTGAN
                     RTQGFAVPDPSGDGAGIDAVVATLSAGGQPPGISTQYFELLTLGVDYTFIIDAVTDSI
                     TGIELFQAIPNTASETLAVSYVNVLEDVIGGTYSSLGVQNPATGQVYPPTSPEGRQLL
                     LEMIKAPDPDPRNPFFGSTWFLEIKNIYNLGLTNIDGTSLEISIVDDLRPRLNPQFPD
                     SSDVPYLQIFGLDQTDRSGTGPPDGRIDLTFGRVNLDAGLLQFPVLEPFIPDPALVAD
                     WTDGRFSFTGPYAAQYDTSRAIYTEKLNPTREEEVNQYSIHVRAVSTSKSFRINALNI
                     VEQSETITLDGQRLNRGSDYDIDYTTGEVTLKDSALSRLNPDSNINIDYQFKPLGGVG
                     SSTLAGMSTTSKFGENARLGTTLLYESRATSSDRARLGEEPTRAIVGGLTGSYQHQSR
                     ILTDIANWLPYVDSDQPSTISLDGEIAGSAPNPNTKNEAYIDDFEGVEDTDRIGLARR
                     SWYPASLPLEETNLAKSDTSRAGFYWYNIEPEFGLHRRDLNPTLDDQENTLLQSLDLE
                     LAAAPAVDDTASYAGVMLGFSGGGLDLSQGQFLEIWVNDFKPVPADRGGKLRIDLGII
                     DENFYELNTNTYHDEDKARDGFAATFDDTGFDGLFNEAEPTLPGGTPDDPSGDDIDLR
                     RINGRYSKVNGTEANLVYDTEDLDRNGQLGRVNAYFSFEIDLADSAEIDIRRQYPGYD
                     GFSDAGHSNDSWRLYRVKLSDYVIRTNTAIQPRLDEIRHVRIWVDDLEEVVRTDGGVG
                     NLRLQIAEFSIEGNRWEIDGVRNLYDGIRENAPTEFAIGVISTKTDPGVYQPPVVPNE
                     QNEIADKESSLALRYAGLRTAEQVRILKRFLGAGLNLTLYRDLNFWVHTDSLRAGTEY
                     YFRMASNETNYYEIAVPFTSTFYNETGWARVVVNLSDLTNLKFAAPDTIVTTGAVDLA
                     DPSRVYPVRMRGAPNLTGVRFLYAGVRNVSNPDSLGGEIWMNDIYVGDVMRDFDHAER
                     VSANFNIAGGAISFGGNWARTGADYRGLRQTRGPGADNTVLGLNAKTDLQYFLPLAGF
                     SLPVSGNYSQTRSLPKFPPNSDTEITDASVSDSLKTVRKTRSFNVSLARRTTSKNFLM
                     RYTLDRLRPTFSYSDQRGASPATRDTTTNMQGSLTYQMTWSGGNTVPLFGRNQFRWWI
                     NQLDLSSSASRQTGKRWSLINGEYRRDPYQYTASLRNQGTVRYNPFRSLETSFTMTQD
                     RDLALDHMWKGYNVGTEIGRTNNMRVSFVSPEWRLLKLFEKPSIEVQSSYSENSGPNV
                     RQVGDPEGTRNVSASRNDTGRIGFDIGKQFGNVFRWVGWDIAKPQAPPRQTVPGGAPP
                     DSANPIPSPPDTTQAKNARPGAGTAFRGLGRILTGIRPIKANITRRKGSSYTRIPDRP
                     DWSYQLGLDNNTGILVDGSGIGPPDTRSANLTYNLDTGVTLRENLDIQGRYSQAINDQ
                     DVRANQNRSTSTTWPDIQARWGGLEKLRVLDQSITQGELRVDWRETTVESGPKDQEPV
                     TTNETFTLTPALVMQWKNQLNSSINVSMTENTSDTRGSRSVNTSTSVGVELKKTFRGG
                     GGLKLFGKGIDWTNEMEATLQLAYSTSGGERFQPGSTLAEPIPKSTALSVNPLVRYTF
                     SRNINGSAFVGYERS"
     gene            6448..7911
                     /locus_tag="VEC56_02340"
     CDS             6448..7911
                     /locus_tag="VEC56_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXV13023.1"
                     /translation="MSRSPMIFERYILRNYLGPFVFSMAVITFVFIMDFILRYIDLFL
                     GKGVKFHIVLQAFALSLGHMFALIIPMAVLPATLMTYGNLTSENEITAMRACGYSTYR
                     MIVPGIFGAALLTVGLILYNNHVLPESNHRLMNLLIDIQRKQPTVELNPNRFIDDVQG
                     YTIYFQEKDDRTGEIQDVQIFKRNEKGGLPTTIIAESGKLQMLEGQNVLRVDLNNGEI
                     HELPVAADRGTYRRTTFKEYTLNIKDVDRSLQRSERDYRGDREMSVRMMKDKIQEIRA
                     DLALSRQEIIRQANASMDNTFSLLDAKSRARRWASKPDSAAASSVEGPPKPRLTVRPS
                     GSAGGRAPLAARGEFLARQRIETEMGKRESFEKQVRRYQVEIHKKYSIPFACIIFVVV
                     GAPLAIRMGRSGMNMAIGLSILFFLVYYVCLIGGEKLADRGLADPFLAMWAPNIIFGA
                     LAIVFLRKAAREQALADWTWVSVLRWLFRRHATANPR"
     gene            7889..8968
                     /locus_tag="VEC56_02345"
     CDS             7889..8968
                     /locus_tag="VEC56_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXV13024.1"
                     /translation="MRPRIHDRYLLARFVRIFLYSVLSFVVIYITVNVFEEIDNFIDH
                     DAQLGEIARYYVFSIPFVLTYVVPVSLLLGTVFAMGILARRNELTALLASGVSLVRVA
                     APIFSVALLVSIGSVFFNDAVVSRANRIKSDIMRHDIEGRERQNPDVKNNFRYLGADG
                     FVYLAETYSHENLAMYDVVVQQFDGNTMERRIDAKNAKWNGSRWVFSSGFVRTFGSEE
                     HVEGFDTLTVREMTETPEDFAQEEIDEENMTFVELQRYIERVQKSGGKVQKYLVDLYF
                     KLSFPFAGSIFVLIGVALSSGKRKPSIATGFGLTLVIAFVYYAILRVGQTLGHNDFLP
                     AALAAQLGNIIFLAVGLAMLRRANR"
     gene            8965..10662
                     /locus_tag="VEC56_02350"
     CDS             8965..10662
                     /locus_tag="VEC56_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXV13025.1"
                     /translation="MITPPRTILFVAAVLTAAAHAHAKPEYKITPGDLTKLDARMQRE
                     VASLQYLMNPYQLRQFFALPSDSARREWITRFWAKNDPTPATPKNEMKTEHYLRADIA
                     QIEFKISTFPGWDKRGEVLIRYGFPDYRGTMPPEVTARKVHAPGELWFYKRHQMIVQF
                     RDESLRGNFQYAITPFGDAEGVSPELMEFLTYDAEETIQEQIPPQYLEFYEAPEINDT
                     GRRWGTLDELFKGLPQDTPLRPRVHGTSENIDEIASPDYERNLPSNPSEVFYQDEARE
                     YAANFEGVLEDTPSSYPFNFAKTAFTFYFGVDQFRGGDGVNRVEVNLEFPVKPVPGKS
                     VPARSYHAEAIVMDERYRVVDKQKRDISLPSTLARADGARLMPAQIVFSLPRGYYRVA
                     VNMSDLDEKTASAYRTNVSARNFDDALALSDVLFAQKIAATGELSPFARGPLEVVPHP
                     IRRYALGTPVPVYFEVYELSLDERGLSDYEVEYRVVPSSDDKKSILERFGGSPTVFAS
                     RFDGSGYNATEPLHITIKSENLKPGLYDFIVRVKDELAQSETFRQASFRIVEKSEKD"
     gene            10701..11393
                     /locus_tag="VEC56_02355"
     CDS             10701..11393
                     /locus_tag="VEC56_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13026.1"
                     /translation="MTSGYWQCGKCGALHTKDPVRRATSEKYIETGAPVLGHETCAKC
                     STRYDAEEIYRGRRDPPQLDSFVAEVISDPTSQWNAALKVWTRKRPLAKDIAVILELD
                     PATVAHYRDSASPLQLAAVALAKTEGEEIDLHSDSTGLVVPGGRITSDFPSWRVWLSA
                     RGHLWVTKKRVAWEDYSESGPVPEALKHAHCAGPVPAIPYLVGLCMAAGLDPNKLSPL
                     EIKRLDEAAAKE"
     gene            complement(11459..12715)
                     /gene="ahcY"
                     /locus_tag="VEC56_02360"
     CDS             complement(11459..12715)
                     /gene="ahcY"
                     /locus_tag="VEC56_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXV13027.1"
                     /translation="MKYDIKDIGLATVGKDRIEWADDRMPVLRSIRDRFQKEKPLNGV
                     RIVACLHVTTETANLMRTLQAGGAQAGLCASNPLSTQDDVAASLVADYQIPVFAIHGE
                     DNDTYYKHINMALDLKPQITMDDGADLVSTLHSSRDDALKGVFGGTEETTTGVIRLRS
                     MQKEGVLKYPIVAVNDAITKHFFDNRYGTGQSTIDGILRATNVLLSGSTLVVAGYGWC
                     GRGVATRARGMGANVIVVEVDPVKALEAVMDGYRVMPGEEAAAIGDVFVTLTGNIAVI
                     RREHFERMQDGAIVCNSGHFNVEIEIEELEKMAVKKKRVRKDVDQYTLKNGKRINILG
                     EGRLINLAAAEGHPASVMDMSFANQALAAEYMVSHHGSLKPEVIPVPERIDREIARLK
                     LVSMGYQIDVLTDQQKRYLASWEQGT"
     gene            complement(12740..13885)
                     /gene="metK"
                     /locus_tag="VEC56_02365"
     CDS             complement(12740..13885)
                     /gene="metK"
                     /locus_tag="VEC56_02365"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXV13028.1"
                     /translation="MGAKRLFTSESVTEGHPDKIADQISDSVLDAIISQDPKSRVACE
                     TLVTTGLAFVAGEITTEAYVDIPSLVRSVIKDIGYTDATFGFDCHTCSVITSIDEQSP
                     DISMGVDREGAGDQGMMFGYACRETEVMMPLPIFLAHRMSERLAWVRRNEASCAYLRP
                     DGKTQVTVEYIDGKPYRIDTVVVSTQHSEDVKDETIRKDMDEKVVRPVLKKYGFPGDG
                     YRLLVNPTGRFVQGGPKADCGLTGRKIIVDTYGGMGSHGGGAFSGKDPSKVDRSASYA
                     ARHIAKNLVAAGLAEECELQIAYAIGEKDPVSVMVDSFGTGKLSDEKLTGIVRETFDL
                     TPKGIIERLKLRRGIYRKTAAYGHFGRDDKEFTWEHTDFVDELKRRA"
     gene            complement(13896..>14376)
                     /locus_tag="VEC56_02370"
     CDS             complement(13896..>14376)
                     /locus_tag="VEC56_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HXV13029.1"
                     /translation="LLDGKGLFAAVREIIDRNITTNNEYQITDALQLMIGRGVVFAPF
                     AIDEWFDCGKPEAMLETNRKLLKNGPSTPPIEGSIIIPPVSISPGAEIVNSIIGPYVS
                     IAERCVIHSSVVRDSVIAEGASVTDALLESSLVGANAVIRGGFKKLDVGDSSEISFK"
BASE COUNT         2435 a         4523 c         4641 g         2777 t
ORIGIN      
        1 gcttcgttcg tagccgacga acgcgctgcc gttgatgttc cgcgagaagg tataccggac
       61 gagcgggttg acgctcaagg ccgtcgattt cggaatcggc tcggccagcg tcgagccggg
      121 ttggaagcgc tcgccgcccg aggtcgagta ggccaactgg agcgtggcct ccatctcgtt
      181 ggtccagtcg atgcccttcc cgaacagctt gagcccgccg cccccgcgaa acgtcttctt
      241 gagctcgacg ccgaccgacg tgctggtgtt caccgagcgc gagccgcgcg tgtcgctcgt
      301 attctcggtc atgctcacgt tgatcgagct gttgagctgg ttcttccact gcatgaccag
      361 cgccggcgtg agcgtgaacg tttcgttggt ggtgacgggc tcctggtcct tgggacccga
      421 ttcgacggtg gtctcgcgcc agtcgacgcg cagttcgccc tgcgtgatcg actggtccag
      481 cacgcgcagt ttctcgagtc ccccccagcg ggcctggatg tcgggccacg tggtggaggt
      541 tgaccggttc tggttggcgc gaacgtcctg gtcgttgatg gcctggctgt agcggccttg
      601 gatatccagg ttctcgcgca gcgtcacgcc ggtatcgagg ttgtaggtca agttggcgct
      661 gcgcgtgtcc ggcggtccga ttccgctacc gtccaccaag atcccggtgt tgttgtcgag
      721 tcccagttgg tacgaccaat cggggcggtc gggaatgcgc gtgtagctcg atcccttgcg
      781 ccgcgtgatg ttggctttga tgggacgaat accggtcaat atgcgaccga gcccgcggaa
      841 cgcggtgccg gctcccggcc gcgcgttctt ggcctgggtg gtgtccgggg gactcggaat
      901 cgggttggcc gagtcgggcg gcgcgccgcc cggtaccgtt tgacgcggag gcgcttgcgg
      961 cttcgcgatg tcccatccca cccagcggaa cacgttcccg aactgcttgc cgatgtcgaa
     1021 tccgatgcgc ccggtatcgt tgcgcgatgc actcacgttg cgcgtgcctt ccgggtcgcc
     1081 gacctggcgc acgttggggc ccgaattctc cgaatagctc gattgcactt cgatgctggg
     1141 tttctcgaac aacttcagca gtctccactc gggagagacg aacgaaacgc gcatgttgtt
     1201 cgtgcgaccg atctcggtgc cgacgttgta ccctttccac atgtggtcga gcgcgaggtc
     1261 tcgatcctgc gtcatggtga acgaggtctc gagcgaccgg aaggggttgt accgcacggt
     1321 gccttggttg cgcaggctcg cggtgtattg gtacgggtcc cgtcgatact ctccgttaat
     1381 gagactccag cgcttccccg tttggcgcga cgccgacgac gacaaatcga gctggttgat
     1441 ccaccaccgg aattgattcc taccgaagag cggcaccgtg ttcccacccg accacgtcat
     1501 ttggtaggtg agacttccct gcatgttggt ggtggtatcg cgggtcgcgg ggctggctcc
     1561 gcgctggtcg ctgtaggaaa acgtcgggcg caagcggtcc agggtgtagc gcatgagaaa
     1621 attcttcgac gtcgtgcgac gggcgagcga gacgttgaag ctgcgcgtct tgcgcaccgt
     1681 cttcaggctg tcgctcaccg aggcgtcggt gatctcggtg tcgctgttgg gcgggaactt
     1741 gggcaatgac cgagtctgcg aatagttgcc cgataccggg agggagaacc ccgccagcgg
     1801 caggaaatac tgaaggtcgg tcttggcgtt gagtcccagt acggtgttgt ccgcgcccgg
     1861 gccgcgtgtt tgcctgagtc cgcggtaatc cgcgccggtg cgcgcccagt tgccgccgaa
     1921 gctgatcgca ccgccggcaa tattgaagtt ggcgctcacg cgctcggcgt ggtcgaagtc
     1981 gcgcatgacg tcgccgacgt agatgtcgtt catccagatt tcgccgccca acgagtcggg
     2041 gttggagacg ttgcgcacgc cggcgtacag gaagcgtaca ccggtcaggt tgggcgcgcc
     2101 tcgcatgcgc accgggtaca cgcgcgacgg atccgccaag tcgaccgcgc cggtggtcac
     2161 gatcgtgtcc ggcgccgcga acttcaggtt ggtcaggtcg gacagattca cgacgacgcg
     2221 tgcccatccc gtctcgttgt agaacgtgct cgtgaagggc accgcgatct cgtagtagtt
     2281 ggtctcgttc gaggccatgc ggaagtagta ctccgtgccc gcgcgcagcg agtcggtgtg
     2341 gacccagaag ttgaggtccc ggtacaacgt gagattgagg ccggcgccca ggaagcgctt
     2401 gaggatgcga acctgctcgg cggtgcgcaa tccggcgtag cgcagcgcga gcgacgattc
     2461 tttgtccgcg atctcgttct gctcgttggg gacgaccggc ggctgataga cgcccgggtc
     2521 ggtcttggtg ctgatgacgc cgatcgcgaa ctcggtgggc gcgttctcgc ggatgccatc
     2581 atagaggttg cgcaccccgt cgatctccca tcggttgccc tcgatcgaaa actcggcgat
     2641 ctggagccgc aggttgccca caccgccgtc ggtgcgcacg acttcttcga ggtcgtccac
     2701 ccagatgcgt acgtggcgaa tctcgtccaa ccgcggctgg atggcggtgt tggtgcgtat
     2761 cacgtagtcg gagagcttca cccggtacag gcgccacgag tcgttgctgt gcccagcgtc
     2821 gctgaagccg tcgtaacccg ggtactggcg acgaatgtcg atctcggcgg agtcggccag
     2881 gtcgatctcg aaactgaagt acgcgttgac gcgaccgagc tgcccgttgc ggtcgaggtc
     2941 ctcggtgtcg tagacgaggt tggcttcggt cccgttgacc ttggagtagc gtccgttgat
     3001 gcgcctcagg tcgatgtcgt caccggacgg gtcgtcgggc gtgccgcccg ggagcgtggg
     3061 ctcggcctcg ttgaacaacc cgtcgaaacc ggtgtcgtcg aaggtggccg cgaagccgtc
     3121 gcgagccttg tcctcgtcgt ggtaggtgtt ggtgttgagc tcatagaagt tctcgtcgat
     3181 gatgcccagg tcgatgcgga gcttgccgcc gcgatccgcc ggcacgggct tgaagtcgtt
     3241 cacccagatt tccaggaatt gaccctggct caaatcgagc cctcccccgg agaacccgag
     3301 catgacaccc gcgtacgacg ccgtgtcgtc gaccgccggc gcagcggcca gctccagatc
     3361 gagcgactgc aggagcgtgt tctcctggtc gtcgagggtc gggttcaggt cgcgccggtg
     3421 cagaccgaac tcgggctcga tgttgtacca gtagaaaccg gcacggctcg tgtccgactt
     3481 ggccagattg gtctcctcga gcggcaagct ggccgggtac cacgagcgtc gcgccagccc
     3541 gatgcggtcg gtgtcctcga ccccctcgaa atcgtcgatg tacgcctcgt tcttggtatt
     3601 cggattggga gcgctgcccg cgatctcgcc gtcgagcgag atcgtgctgg gctggtcgct
     3661 gtcgacgtag ggcagccagt tagcgatgtc ggtcaagatc cgcgattggt gctggtagct
     3721 tcccgtgagc ccgcccacga tggcgcgcgt gggctcttcg cccaagcgcg cccggtcgct
     3781 gctggtggcg cgcgattcgt ataacaatgt ggttccgaga cgcgcgttct cgccgaactt
     3841 ggaggtcgtg ctcatgccgg ccagggtgct cgatcccacc cctcccagcg gtttgaactg
     3901 atagtcgatg ttgatgttcg agtctggatt caagcgcgac agcgccgagt ccttgagcgt
     3961 gacctcgccc gtggtgtagt cgatgtcgta gtcgctgccg cggttcaaac gctgcccgtc
     4021 caacgtgatg gtttcgctct gctccacgat attgagcgcg ttgatgcgga acgacttcga
     4081 cgtactgacc gcacgcacgt ggatcgagta ctgattgacc tcttcttcgc gcgtcggatt
     4141 gagcttttcg gtgtagatcg cgcgcgaggt gtcgtactgc gccgcgtagg gccccgtaaa
     4201 cgagaacctg ccgtccgtcc aatccgccac cagggctggg tcggggatga acggctcgag
     4261 aaccggaaac tgcagcaggc ccgcgtcgag gttgacacgt ccgaacgtga gatcgatgcg
     4321 gccgtcgggc ggacccgtgc ccgaacgatc ggtctggtcg agtccgaaga tctgcaagta
     4381 cggaacgtcg gagctgtcgg gaaactgcgg gttcaagcgc gggcgcaggt cgtcgacgat
     4441 gctgatctcg agcgaagtac cgtcgatgtt ggtcagaccc aggttgtaga tgttcttgat
     4501 ctcgagaaac caggttgagc cgaagaacgg attccgaggg tccgggtcgg gcgccttgat
     4561 catttcgagc aggagctgcc gaccctcggg cgacgtgggc gggtagactt gtccggtcgc
     4621 cgggttttgg acgccgagcg acgaatacgt cccgcctatg acgtcctcca gcacgttgac
     4681 gtagctgacg gccagcgtct cgctggcggt attggggatg gcctggaaca gctcgatgcc
     4741 ggtgatcgag tcggtcaccg cgtcgatgat gaaggtgtag tccacgccca gcgtgagcag
     4801 ctcgaagtac tgcgtggaga tgccgggtgg ctggcccccg gcgctgaggg tcgcgaccac
     4861 cgcatcgatg ccggctccat cgccgctcgg gtccggcacc gcgaacccct gcgtgcggtt
     4921 cgcgcccgtc ccggtattat tgttgatgaa gcccttgtag acctcgatcg tcgccgcctg
     4981 cggcccgatg aaggcgcgcg ggttgtcgaa gtagaagtac ttgttgtgca cgtagtcgac
     5041 gtcgcgaatc tcgcggaact cggtctcgcc caaggcaccg ccggtgggcg tgaacgtcga
     5101 ggaggagact tcgccttcct gcttgctcgc gatcatcgtg aaatccgtcg accctatctt
     5161 ggccagcatc ttggcgccga aaagaccctg cgcgttggtc gacacgctga ccaactgcga
     5221 gcccggcagc gaaaggctgg tgttgccgag ctcgatcaac tggatcactt cgtcttcgta
     5281 gccggtgtag gcgaggcgca cgcggttggc gtcgtctccg atggcttcgc tggagtggtc
     5341 cacttggatg ctcaccttgt cgccgatggt gcccgtcaga ctcacgtcca gctgttgctg
     5401 catgtcgagc gacgggaaca gcgactgctc ctgccggccc tcggcgccaa tgaacggatc
     5461 gagcacggtg gtctcgcccg cgatcgtgat ctcctcgcgg ccgcgcagcg tgatgctggt
     5521 cttttctccg ggcccgaaga tagactcgag ttgcttgggc atggggagcg gaatgtcgaa
     5581 ggtgatgccg ccggtggtgg cctcagtggt caccgccagg gccgccacac tcgcgcgcgc
     5641 cgattcgcgc cagacttggc gcgtggtgtt gcgatagatc tcggtcgcga cgtcgtccat
     5701 cgggcgcaag tcgacggcga gatcggggat ggtgtagtcg cgcggctcgg tcaggatgag
     5761 atcgcccgcc tggcgctcgg tggcttcgaa gaacgcgccg cgcggcaccg tgatgcgcgc
     5821 ccggtagccg ggtaccgcga ggtagtcgtc gaccgcgcgt gtggtcgaca cgtcgctggt
     5881 caggaagtgc ggtacctggt agcgaagacc gccgaagcgg atgcggtggt agtcgtagac
     5941 caccaccgag tcggcgattt cgtcggttac gcggtcgcga tagagggtgt catagagctt
     6001 gccgacctcg cccaagtcga tcgagtaagc cgcgaaccct tgtgcgcgcg cggtgttaac
     6061 aggagcgagg agggaaacaa atgcgagagc caagaccggg gtccagaggc ggaaggtcgc
     6121 atgcgagatt ctggacaaca ccgaaataca atggcgcggc acgtcgatac caatcgttca
     6181 gaagtttcga gatcaccttc tcgtgggtgt cgtgcatcgg ttcggtcgca ctcgtcgcgt
     6241 cccgattcga ggacgaaacg agccgaaagt gtcgccgttc ggcgacaggt cgggacaaac
     6301 ggctagacgc ccccacctac ccggtccgtt cgccttcgcg cggcctcgaa gcgtgcgcga
     6361 tgccaggggg cgtcgtcgat aaaacgtctt gtcagcaaac ccgttatcag tgattatgca
     6421 gaggtctcta ggaaccgtcc ctcccgaatg agccgtagcc cgatgatctt cgagcgctac
     6481 atcctgcgaa actacctcgg ccccttcgtc ttctcgatgg cggtaataac cttcgtgttc
     6541 ataatggatt tcatcctccg ctacatcgac ctgttcttgg gcaagggggt caagtttcac
     6601 attgtgctgc aagcatttgc gctctcgttg gggcacatgt tcgcattaat cataccaatg
     6661 gccgtattgc ccgcgacgct aatgacttac ggcaatttga cgagcgaaaa cgagatcacg
     6721 gccatgaggg cgtgcggcta cagcacgtat cggatgatcg tccccgggat cttcggcgcg
     6781 gcgttattga ccgtcgggtt gatcctctac aacaaccacg tgctgcccga atcgaaccac
     6841 cgcctgatga atctcttgat cgacatccag cgcaagcagc ccacggtcga gctgaatccg
     6901 aatcgtttca tcgacgacgt gcagggctac accatctact tccaggagaa ggacgaccgc
     6961 acgggcgaga tccaggacgt tcagattttc aagcgcaacg agaagggcgg gctcccgacc
     7021 acgatcatcg ccgagtccgg caagctccag atgctcgagg ggcagaacgt gctgcgcgtc
     7081 gacttgaaca acggcgagat acacgagctg ccggtggcgg cggaccgcgg cacgtaccgg
     7141 cgcacgacct tcaaggagta cacgctcaac atcaaggacg tcgaccggtc gctgcagcgc
     7201 agcgagcggg actaccgcgg cgaccgcgag atgagcgtgc gcatgatgaa ggacaagatc
     7261 caggagattc gggcggacct ggcgctttcg cgccaggaga tcatccgcca ggcgaacgcg
     7321 tccatggaca acacgttttc gctgctggac gccaagagcc gcgcgcgtcg ctgggcatcc
     7381 aagcccgata gcgcggcggc ctcctcggtc gagggtccgc ccaagccgcg gctgactgtg
     7441 cggccgagcg ggtccgccgg cggtcgcgcg ccgctcgcgg ctcgcggcga gttcctcgcg
     7501 cgccagcgca tcgagaccga gatgggcaag cgcgagtcgt tcgagaagca ggtccgccgc
     7561 taccaggtgg agatccacaa gaagtactcg atccccttcg cgtgcatcat cttcgtggta
     7621 gtgggggccc cgctcgccat ccgcatggga cgctcgggca tgaatatggc gatcgggctg
     7681 agcatcctgt tcttcctggt ttactacgtg tgcctgatcg gaggcgagaa gctggccgac
     7741 cgcgggctcg ccgacccctt cctcgccatg tgggcgccca acatcatctt cggggcgctc
     7801 gcgatcgttt tccttcgcaa ggccgcacgc gaacaagcgc ttgcggactg gacctgggtt
     7861 tccgtgctaa gatggctctt ccgccgccat gcgaccgcga atccacgata gatacctcct
     7921 cgcccgcttc gtcagaatct ttctctattc ggtactgtcg ttcgtcgtga tctacatcac
     7981 ggtgaacgtg ttcgaggaga tcgacaactt catcgaccac gacgcgcagc tgggcgagat
     8041 cgcgcgctac tacgtgtttt cgatcccgtt cgtgctgacc tacgtggtgc cggtgtcgct
     8101 gttgctcggc accgtgttcg cgatgggaat cctggcccgc cgcaacgagc tcaccgcact
     8161 gctggcgtcg ggggtgagcc tggtgcgcgt ggccgcaccg atcttctcgg tcgccctgct
     8221 cgtgagtatc ggctccgtgt tcttcaacga cgccgtggtc tcgcgcgcca accggatcaa
     8281 gagcgacatc atgcgccacg acatcgaggg ccgcgagcgc cagaacccgg atgtgaagaa
     8341 caatttccgc tacctgggcg ccgacggctt cgtgtacttg gcggagacct acagccacga
     8401 gaacctggcc atgtacgacg tcgtggtcca gcagttcgac ggcaacacca tggagcggcg
     8461 catcgacgcc aagaacgcca agtggaacgg gtcgcgctgg gtgttctcga gcggcttcgt
     8521 gcgcaccttc ggaagcgaag agcacgtcga gggcttcgac acgctgaccg tgcgcgagat
     8581 gacggaaacg cccgaagact tcgcccagga ggagatcgac gaggagaaca tgaccttcgt
     8641 cgagctccag cgctatatcg agcgcgtgca gaagagcggc ggcaaggtcc agaagtacct
     8701 ggtggacctc tacttcaagt tgagcttccc cttcgcgggg tcgatcttcg tgctgatcgg
     8761 tgtcgcgctg tcgtcgggca agcgcaaacc gtcgatcgcg accggcttcg ggctgacgct
     8821 ggtgatcgcg ttcgtctatt acgcgatcct gcgcgtcggg cagacgctgg gccacaacga
     8881 tttcctgccc gccgccttgg ccgcgcagct cgggaacatt atctttctgg ccgtcggcct
     8941 cgcaatgttg aggcgcgcca accgatgatc acgccgccgc gcaccatcct cttcgtggcc
     9001 gccgtcttga cggcggcggc gcacgcccac gccaaacccg agtacaagat cacgcccggt
     9061 gacctcacga agctcgacgc gcgcatgcag cgcgaagtcg cctcgctcca gtacctgatg
     9121 aacccctacc aactccgcca gtttttcgcg cttccgtcgg attccgcgcg gcgcgagtgg
     9181 atcacccgct tctgggccaa gaacgacccc acacccgcga ccccgaagaa cgagatgaaa
     9241 accgagcact acctgcgggc cgacattgcg cagatcgaat tcaagatctc cacgttcccg
     9301 ggctgggaca aacgcggcga ggtactcatt cgctacggat tccccgacta ccgcgggacc
     9361 atgccgcccg aggtgaccgc gcgcaaggtg cacgcaccag gcgaattgtg gttctacaaa
     9421 cgccaccaaa tgatcgtgca gttccgcgac gagagcctgc gcggcaattt ccagtacgcg
     9481 atcacgccct ttggcgacgc cgagggtgtc tcgcccgagc tgatggagtt cctgacctac
     9541 gacgccgagg agaccatcca ggagcagata ccgccgcagt acctcgagtt ctacgaagcc
     9601 cccgagatca acgacaccgg tcgccgctgg ggcacgctcg acgagttgtt caaaggcctc
     9661 ccgcaagaca cgccgctgcg gccgcgcgtc cacggcacca gcgagaacat cgacgagatc
     9721 gccagccccg actacgagcg caacctccct tccaacccct cggaggtgtt ctaccaggac
     9781 gaagcccgcg agtacgcggc caacttcgag ggcgtcctcg aggacacgcc gtcgtcgtac
     9841 ccgttcaatt tcgcgaagac cgcgttcacg ttctacttcg gggtcgacca gttccgcggc
     9901 ggcgacggtg tcaatcgggt cgaagtgaac ctggagttcc cggtcaagcc ggtgccgggc
     9961 aaatccgttc cggcccgctc gtaccacgca gaagcgatcg tcatggacga acgctatcgt
    10021 gtcgtcgata agcagaaacg agacatctcg cttccgtcga cgctcgcgcg cgccgatggc
    10081 gcgcgtttga tgccggccca gatcgtgttc tcgctccccc gcggctacta ccgggtggcc
    10141 gtcaacatgt cggacctcga cgagaagacc gcgtcggcct atcgcaccaa cgtctcggcg
    10201 cgcaatttcg acgacgcgct cgcgctcagc gacgtgttgt tcgcccagaa gatcgcggcc
    10261 acgggcgagc tgtcgccctt cgcgcgcggg ccgctggagg tggtcccaca cccgattcgt
    10321 cgctacgcgt tgggaactcc ggtgccggtc tacttcgagg tgtacgagct atcgctcgac
    10381 gaacgcgggc tctccgacta cgaggtggag taccgagtgg taccgagctc ggacgacaag
    10441 aagagcatcc tggaacgctt cggcggcagt ccgaccgtgt tcgcgtcgcg cttcgacgga
    10501 tccggctaca acgcgaccga accgctccac atcaccatca agagcgagaa cctgaagccc
    10561 ggtctgtacg acttcatcgt gcgcgtcaag gacgagctcg cgcagtccga aacgttccgg
    10621 caggcgtcgt tccggatcgt cgagaagagc gagaaagact gatgctcgcg ggcagcgctg
    10681 ctgtgctatc ctagccgcgt atgaccagtg gctactggca gtgcgggaaa tgcggcgcgt
    10741 tgcataccaa ggacccggtg cgacgcgcga cctccgaaaa gtacattgag acgggcgcac
    10801 ccgtgctcgg ccacgagacg tgcgcgaagt gcagcacgcg ctacgacgcc gaggagatct
    10861 atcgcggccg gcgcgatccg ccgcagttgg actctttcgt cgcggaggtc atctccgacc
    10921 cgacctcgca gtggaacgcg gcgctcaagg tgtggacgcg caagcgtccg ctcgcgaagg
    10981 acattgccgt gatcctcgag ctcgaccccg ccaccgtcgc gcattaccgc gactccgcgt
    11041 cccccctcca gctcgccgcc gtcgccctgg ccaagacgga gggcgaagag atcgacctcc
    11101 actccgacag caccggcctc gtcgtccccg gcgggcgcat taccagcgac ttccccagct
    11161 ggcgcgtgtg gctctccgcg cgcggtcact tgtgggtcac gaagaagcgg gtggcgtggg
    11221 aggactattc cgaatccggc cccgtccccg aagccctgaa gcacgcgcac tgcgcgggtc
    11281 cggtaccggc cattccctac ctcgtgggtc tgtgcatggc cgccggactg gaccccaaca
    11341 agctctcgcc cctcgagatc aagcgcctcg acgaagccgc cgccaaggag taagaaaaag
    11401 cgccgccgac taccgacgtc gacggcgctt cgttgcgcga atgcttgtcg cgaccccgct
    11461 aggtcccctg ctcccagctg gccagatagc gcttctgctg gtcggtgagc acgtcgatct
    11521 gatagcccat cgagaccagc ttcaagcgcg cgatctcgcg gtcgatgcgc tccggcaccg
    11581 ggatcacttc gggtttgagc gagccgtggt ggctcaccat gtactccgcg gccagcgcct
    11641 ggttggcgaa actcatgtcc atcaccgacg cggggtggcc ttcggccgcg gccaggttga
    11701 tcaagcgccc ttcgcccagg atgttgatgc gcttgccgtt cttgagcgtg tactggtcga
    11761 cgtccttgcg cacgcgcttc ttcttgaccg ccatcttctc gagctcttcg atctcgatct
    11821 cgacgttgaa atggcccgag ttgcacacga tggcgccgtc ctgcatgcgc tcgaagtgct
    11881 cgcgtcggat gaccgcgatg ttgccggtca gcgtgacgaa cacgtcgccg atggcggccg
    11941 cttcctcgcc gggcatgacg cgatagccgt ccatgaccgc ttccagcgcc ttgaccgggt
    12001 cgacttcgac cacgatcacg ttggccccca tgccgcgcgc gcgtgtggcg acaccgcggc
    12061 cgcaccagcc gtacccggcc accaccaggg tcgacccgct caacagcacg ttggtggcgc
    12121 ggaggattcc gtcgatggtg ctctggccgg tgccgtagcg gttgtcgaag aagtgcttgg
    12181 tgatggcgtc gttgaccgcg acgatcgggt acttcagaac gccttccttt tgcatgctgc
    12241 gcaggcgaat gacgcccgtg gtggtctctt cggttccgcc gaacacaccc ttgagcgcgt
    12301 cgtcgcggct cgagtggagc gtcgagacga ggtcggcgcc gtcgtccatg gtgatctgcg
    12361 gtttgaggtc gagcgccatg ttgatgtgct tgtagtaggt gtcgttgtcc tcgccgtgga
    12421 tcgcgaacac gggaatctgg tagtccgcca ccaggctggc cgcgacgtcg tcctgagtgg
    12481 agagtgggtt cgacgcgcac agccccgcct gcgccccgcc cgcttgcagc gtgcgcatga
    12541 ggttggcggt ctcggtggtt acgtgcaggc acgcgacgat gcgcacgccg ttgagcggct
    12601 tctctttctg gaagcgatcc cggatgctgc gcaggaccgg catgcggtcg tccgcccact
    12661 cgatgcgatc tttaccgacg gtggcgagcc cgatgtcctt gatgtcgtac ttcattctgg
    12721 atctcgttcc tttctttgat tacgcgcgcc gtttcagttc gtctacgaag tcggtgtgct
    12781 cccaggtgaa ctccttgtcg tcgcggccga agtgcccgta cgcggcggtc ttgcgataga
    12841 tgccgcggcg cagcttcagg cgctcgatga tgcccttggg agtcaggtcg aaggtctcgc
    12901 gcacgatgcc cgtgagcttc tcgtccgaca gcttgccggt cccgaacgaa tcgaccatca
    12961 cggagaccgg gtccttctcg ccgatcgcgt acgcgatctg gagctcgcac tcctcggcga
    13021 gtccggcggc gaccaggttc ttcgcgatgt ggcgcgctgc gtacgaggcg ctgcggtcca
    13081 ccttgctcgg atccttgccc gagaacgcgc cgccgccgtg gctgcccatg ccgccgtagg
    13141 tgtcgacgat gatcttgcgc ccggtcaagc cgcagtcggc cttggggccg ccctgcacga
    13201 agcgcccagt cggattgacc agcaggcgat agccgtcgcc cgggaacccg tacttcttca
    13261 agaccggccg caccactttt tcgtccatgt ccttgcgaat ggtctcgtcc ttgacgtctt
    13321 cggagtgctg cgtcgagacc accacggtgt cgatgcggta cggtttgccg tcgatgtact
    13381 cgaccgtcac ctgggtcttg ccgtccggcc gcaagtacgc gcacgacgcc tcgttgcgcc
    13441 gcacccacgc caaacgctcc gacatgcggt gcgcgaggaa gatgggcagg ggcatcatca
    13501 cttcggtctc gcggcacgcg tagccgaaca tcatgccctg gtcgccggcg ccctcgcggt
    13561 cgacgcccat cgagatgtcg ggcgactgct cgtcgatcga cgtgatcacc gagcaggtgt
    13621 ggcagtcgaa gccgaaagtc gcgtcggtgt agccgatgtc cttgatgacg ctgcgtacga
    13681 gcgagggaat gtcgacgtag gcctcggtgg tgatctcgcc ggccacgaag gccagcccgg
    13741 tggtcaccag ggtctcgcac gccacgcgcg acttggggtc ctgggaaatg atcgcgtcca
    13801 ggaccgagtc ggaaatctgg tcggcgatct tgtcgggatg accttccgtc accgactcgg
    13861 aagtaaacag tctcttcgcg cccatcgttt ctcctctact tgaagctaat ttcgctcgag
    13921 tctccgacgt cgagcttctt gaagcccccg cgaatcaccg cgttggcgcc caccaggctc
    13981 gattcgagca acgcgtcggt gaccgacgca ccctccgcga tgacggaatc gcgcacgacc
    14041 gaggagtgaa tgacacaccg ctcggcgatc gacacgtacg gcccgatgat ggagttcacg
    14101 atctcggcgc ccggcgaaat ggacaccggc gggatgatga tcgaaccctc gatggggggc
    14161 gtgctggggc cattcttcaa caattttcga ttggtttcaa gcatcgcttc gggttttccg
    14221 cagtcgaacc actcgtcgat ggcgaagggg gcgaacacga ccccccggcc gatcatgagc
    14281 tggagggcgt cggtgatctg gtactcgttg ttggtggtga tgttgcggtc gatgatctcg
    14341 cggacggcgg cgaatagccc cttgccgtcc aacagg
//
LOCUS       DASPGK010000087        10484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_22022, whole genome shotgun sequence.
ACCESSION   DASPGK010000087 DASPGK010000000
VERSION     DASPGK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22022"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VEC56_02375"
     CDS             <1..514
                     /locus_tag="VEC56_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXV13030.1"
                     /translation="SYLPLSKEESTLIVDRFVAVTRASLVSSIVIGVIQGVLGGLAFS
                     IAGIKGAVFWGTLMGVAALVPGVGATVVWLPGVLYLILSGHIAAGLLLFAFCAVIVGS
                     VDNVIRPRLVGRDTKLPELIVLLSTLGGIMLMGPIGFIIGPVVAALFITVWDIYATFL
                     RGPAESSSES"
     gene            543..2285
                     /gene="sppA"
                     /locus_tag="VEC56_02380"
     CDS             543..2285
                     /gene="sppA"
                     /locus_tag="VEC56_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXV13031.1"
                     /translation="MNFFKMVLAVLVAQVLLAFVLFFGLGVMTALFTSGDTTVHVTDG
                     SWLVIDLYGEIPPYDEPESIAGSIFAEPETLAELLVNLEKAAADERLAGVVMKISSSN
                     SLGLASLGELRTSIARVRESGKPVIAFSDDLDRNSLYLAAACDSIFMPNVADLTFTGY
                     GAVESFYKGTLDKLGVHQNLHKIDEYKTAAEPFQRDSMSPENREMTGWLLQEVWEVEL
                     GAISRDRGLSMDSLVACMDHALFTPEEAKQVGLIDGVVYWDELEKRLSDEDELPVVTS
                     AEYGDVLRADVGLKGKSRIAVVHAYGFIGGRESRTDPGLGIMMGHETVVENLRAAADD
                     DRVDAVVLRVDSPGGESLASQIIAREVGKIAAEKPIVVSMGDVAASGGYAIAYPATKI
                     VADSLTITGSIGSIYGKLNIAGAWNKIGITFDSVTKGPNALLWSTVHDFDQAQWKRIE
                     AHHHQSVDQWLAEISKARNIPVEELRGYAEGRVWTGRQAKERKLVDEVGGFSRAIEVA
                     KEAAGIPIDEEVTLDYFPKKRGLYALLMSGDAPITLLRGVVVRALRQDAAETMRVLQR
                     GGEWRLWTGAQPVE"
     gene            complement(2256..3380)
                     /gene="solA"
                     /locus_tag="VEC56_02385"
     CDS             complement(2256..3380)
                     /gene="solA"
                     /locus_tag="VEC56_02385"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HXV13032.1"
                     /translation="MKPFDAAVVGLGAMGSAALCHLARRGARVVGFDAYTPPHALGSS
                     HGETRMIREAYYEDARYVPLLRRAYALWHDLARDAATELIVETGGIFAGPPDAELVAG
                     VERAGREHGIAVERLDGAALAARAPWLRLPSDMVALSEARAGLLHPERCIDAHLRLAV
                     AAGASIHADESVIAWRASGDGVVVETKSGRYPAARLILCAGAGMTAWLADAGVAATVT
                     RQPMFWFTNGRDAASLNQVWAIQIDGERLLYGFPDVGGGLKAAIHYGGAPTTWQSVDR
                     AVSADEARETADLLARFLPGVHGDLLRAVACIYTNTKDHHFAIDAHPAHANVLVVSAC
                     SGHGFKFASAIGEASSQWALDGAWKLDLSLFSLDRLRAGP"
     gene            3426..3866
                     /locus_tag="VEC56_02390"
     CDS             3426..3866
                     /locus_tag="VEC56_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXV13033.1"
                     /translation="MRLRSVLFVIVLVLPLASAHADEVFVASLSGARVGSPATGAATF
                     TLRADLSAIDYVVTYDNLSSPEYMAHVHRADDTVLIFLPLGTPKIGTWEAPSAQDIAE
                     LRNEELYVNVHSEYYPTGEIRGTLRRQALPAAVTTWGAIKALYR"
     gene            complement(3863..4762)
                     /locus_tag="VEC56_02395"
     CDS             complement(3863..4762)
                     /locus_tag="VEC56_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV13034.1"
                     /translation="MVFDAHIHFFGREFYAFQATFVPREDPETVLGRVRAGGLEIPGP
                     DPKVHAARWIAELDKNRIDRAVLFASCPTEMKSVGEAAASHPRRLFPYTVINPTAPAT
                     LQALESMQSTYHFKGVLLFPSMHEFSIGSPEVAKVIEVAKRHHLVVFVHCGKLRVNVR
                     KLVGLNADFPAERSRPRDLIPVARANPDVRFVVPHFGSGWFEEALALGATCPNVYTDT
                     AGSNAWILEHDPPLSLAEVFQAARVSFGVDRMLFGSDSGVFPRGYRADVLTAQQETMR
                     AAGFSNDEQRMVLGGNLTRLLEV"
     gene            4897..5976
                     /gene="amrS"
                     /locus_tag="VEC56_02400"
     CDS             4897..5976
                     /gene="amrS"
                     /locus_tag="VEC56_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019589581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXV13035.1"
                     /translation="MNHTVSTRYWHLLDDGRVQCDVCPRFCKLREGQRGMCFVRAREG
                     DAIVLTTYGSSSGFCIDPIEKKPLNHLLPGSAVLSFGTAGCNLACKFCQNWDISKSRE
                     VETLSEEATPEAIARAAVETGCRSVAFTYNDPVVFMEYAIDVAKACHERGILTVAVTA
                     GEICDEPRREFYRYMDAANVDLKGFTEDFYWRVCKGDLKTVLDTLVYLKRETSVWFEI
                     TTLLIPGENDSSDEIEAECAWIREHLGLDVPLHFTAFHPDFMMLDKPPTPPETLTRAR
                     DIALKQGLRYVYVGNVHDESGSSTYCPKCGTTLIGRDWYRLTAWNLDSRGCCKKCGTQ
                     IPGRFEAKPGTWGARREAVNMRAFA"
     gene            6093..7184
                     /gene="hppD"
                     /locus_tag="VEC56_02405"
     CDS             6093..7184
                     /gene="hppD"
                     /locus_tag="VEC56_02405"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HXV13036.1"
                     /translation="MKPPFKLLGFDHVTFAVGNAKQAAVYYQSVFGFDLVGYRGLEQG
                     DRQTASYVLRQNHVTFVLNTPYTPSSSLNNLLAKHGDGATDIAFAVDDAVKAWEHTTK
                     HGAKNWFEPRTMEDEGGKVILSGIHTYGDVTHTFVQREGYKGVFLPGYAPAKSRGEVR
                     QTGLLFIDHIVGNQPDSEMEDVVRFYENIFGFHRLWTVDDKDISTEYTALRSVVVADD
                     NENVKMPINEPASGLKKSQIQEYVEFNSGAGVQHIAMSTNDIIGTIKQLTANGAEFLT
                     VPGSYYDELPKRVGDIDEEIPTLRKLGILVDRDDSGYLLQLFTKPVQDRPTLFFELIQ
                     RKNARSFGKGNFKALFESIEREQARRGTL"
     gene            7197..8351
                     /locus_tag="VEC56_02410"
     CDS             7197..8351
                     /locus_tag="VEC56_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HXV13037.1"
                     /translation="MPIYIQRGKIPHRRHTVFKSPQGKHYYEEHISRLGFSDIYSNVY
                     HVHMPTRVAKVGKFRPVTLEPLEAEHRHRHLKTFDLKPEGNWLTGRRPMMFNADVILG
                     TTSPVRGASDFFYKNATCDEVHFVHKGRGVLDSMFGRLAFGEGDYVVIPRGVIHKMTF
                     ESDDNRVFFVESSGPVDWPKSYRNQYGQLLEDAPYCERDIRTPEFVEAMDKPGEFPMH
                     IRLRDGIQEYLWGQHPFDLVGYDGFYYPWIFNIKDYMSKVGRVHLPPPTHLTFTGPGY
                     VLCSFCPRPFDFEEGAVPIPYAHSNVDSDEVIYYVDGSFMSRKGIGVGSITLHPYGIP
                     HGPQPGLLEQSLGAKETGELAVMVDTFRPLKVAKVCATIDDANYPYSWQY"
     gene            8380..9006
                     /locus_tag="VEC56_02415"
     CDS             8380..9006
                     /locus_tag="VEC56_02415"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXV13038.1"
                     /translation="MIIDPATTRKQEFYPWLIRCIVPRPIAWVSTISREGVPNLAPFS
                     FFTAISTEPPTLCFAPGRHAVTGKKKDTLVNIEATGDFVVNVVPEELAEAMNETATDY
                     PHGVSEFAEARLTPVPAERVKAPRLAESPIHFECERYEIVQVGPDGVGGGALVIGKIV
                     LLHVDDRVITDGKVDYDLFRALGRLGGMEYTRTHDRFQMARKKYTPRS"
     gene            9294..9722
                     /locus_tag="VEC56_02420"
     CDS             9294..9722
                     /locus_tag="VEC56_02420"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXV13039.1"
                     /translation="MCKSRNRLSQAGYTLMELMIVVAVVGILAAIAVPSFSGYLKRTR
                     VVGARNQLITDCYLARSLAISRRETITMVFSADQYQIVNGGGTVFRTTPAQSGVTFNA
                     SDDPNFYAWGLADAVDIAIDGPSNSTDVSLSPTGVASHGY"
     gene            9712..10086
                     /locus_tag="VEC56_02425"
     CDS             9712..10086
                     /locus_tag="VEC56_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV13040.1"
                     /translation="MVTKRVANAHHRQEGFSLVELMVALVILTIGLLPLAFVQTRAQQ
                     DVFDSGRYTEAITIATLQMEETKSLGFGVAVTDTGTVDNYTWVRDVQSISPTLDQITV
                     SVSWTERGTPRLVQIINRLSDR"
     gene            10091..>10484
                     /locus_tag="VEC56_02430"
     CDS             10091..>10484
                     /locus_tag="VEC56_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV13041.1"
                     /translation="MSKHARKIRNAFSDNRGITLVELVVTVIILGIVLLVVNQVFFST
                     TRIYGSTSTRANQQMNARAGLSVMVTELRPAGSDPAEQGIQGVLAAQSDTVHVQADLT
                     GDGAIQTAEPSEDIMYYYDSNAQAVMREI"
BASE COUNT         1905 a         3422 c         3378 g         1779 t
ORIGIN      
        1 ctcctatttg ccgctgtcca aggaagagag cacgctcatt gtcgatcgct tcgtcgcggt
       61 gacgcgcgcg tcgctggtta gctcgatcgt gatcggcgtc atccagggtg tgttgggcgg
      121 cctcgcgttc tccatcgcgg gcatcaaggg cgcggtgttc tggggaacgc tcatgggagt
      181 agccgcgttg gtgcccggcg tgggggccac cgtggtgtgg ctgccgggcg ttctctacct
      241 catattgagc ggtcacatcg ccgccggctt gctattgttc gcgttctgcg ccgtgatcgt
      301 gggcagtgtc gacaacgtga ttcggccgcg cctggtcggg cgcgacacca agctccccga
      361 gttgatcgtg ctgttgtcca cgctgggcgg catcatgctg atgggcccga tcggcttcat
      421 catcgggccg gtggtggccg cgttgttcat tacggtttgg gacatctacg ccaccttctt
      481 gcgcggcccg gcggaatcgt cgtcggaatc gtaaccgcgc gcgaatcgaa agggattgtg
      541 cgatgaactt cttcaagatg gtactcgcgg tgttggtggc gcaggtgctg ctcgccttcg
      601 tcttgttctt cggcctgggt gtcatgaccg cgctgttcac gagcggcgat accacggtgc
      661 acgtaaccga cgggtcgtgg ctcgtgatcg atttgtacgg cgagatcccg ccttacgacg
      721 agcccgagtc gatcgcgggc tcgatcttcg ccgagccgga aacgctggcc gaactcctcg
      781 tcaacctcga gaaggcggcc gccgacgaac gcctcgcggg cgtggtgatg aagatctcct
      841 ccagcaactc actggggctc gcgtcgctgg gcgaactgcg cacgtcgatc gcgcgcgtgc
      901 gcgagagcgg taagccggtg atcgccttct ccgacgacct cgaccgcaat tcgctctacc
      961 tggccgccgc gtgcgattcg atcttcatgc ccaacgtggc cgacctgacc ttcaccgggt
     1021 acggcgcggt ggaatcgttc tacaagggca cgctcgacaa gctcggcgtg caccagaatc
     1081 tccacaagat cgacgagtac aagaccgccg cggagccgtt ccagcgcgac agcatgtcgc
     1141 cggagaatcg cgagatgacg gggtggctgc tgcaggaagt ctgggaagtc gagctgggcg
     1201 cgatctcgcg cgaccgcggg ttgtctatgg actcgctggt cgcatgcatg gaccacgcgc
     1261 tcttcacgcc cgaggaagcc aagcaagtcg gactcatcga cggcgtggtg tattgggacg
     1321 agctggagaa gcgcctgagc gacgaggacg agctgcccgt cgtcaccagt gccgagtacg
     1381 gcgacgtcct gcgcgccgac gtcggcctca agggcaagag ccgcatcgcg gtggtgcacg
     1441 cgtacgggtt catcggcggg cgcgagagcc gcaccgaccc cggcctgggc atcatgatgg
     1501 gccacgagac ggtggtcgag aatctgcgcg ccgcggccga cgacgaccgc gtggacgcgg
     1561 tggtgctgcg cgtcgacagc ccgggcggcg agagcctcgc cagtcagatt atcgcgcgcg
     1621 aagtgggcaa gatcgcggcc gagaaaccga tcgtggtgtc gatgggcgat gtcgcggcgt
     1681 cgggcggcta cgccatcgcg tatcccgcca ccaagatcgt cgccgactcg ctgaccatca
     1741 cgggctcgat cgggtcgatc tatggcaagc tcaacatcgc gggggcgtgg aacaagatcg
     1801 gcatcacctt cgactcggtg accaaagggc ccaacgcgct cttgtggtcg accgtgcacg
     1861 acttcgacca ggcgcagtgg aaacgcatcg aggcccacca ccaccaatcg gtggatcagt
     1921 ggctggccga gatctccaag gcgcgcaaca tcccggtcga agaattgcgc ggctacgcgg
     1981 agggccgcgt gtggacgggc cggcaggcca aggagcgcaa gctcgtcgac gaggtgggcg
     2041 gcttctcacg cgcgatcgaa gtggcgaaag aggcggcggg gattccgatc gacgaagaag
     2101 tgaccctcga ctacttcccc aagaagcgcg ggctctacgc gctgctcatg agcggcgacg
     2161 cgcccattac actcctgcgc ggcgtggtgg tgcgtgcgct gcgccaagac gccgcggaga
     2221 cgatgcgcgt cctgcagcgc ggcggcgagt ggcggctatg gaccggcgcg cagccggtcg
     2281 agtgagaaga gcgagaggtc cagcttccag gcaccgtcca gcgcccactg cgacgacgcc
     2341 tctccgatcg cactcgcgaa cttgaagccg tggcccgagc acgcgctcac gacgagcacg
     2401 ttcgcgtgcg cggggtgggc gtcgatcgcg aagtggtggt ccttcgtatt cgtgtaaatg
     2461 cacgcgaccg cgcgcaagag gtcgccgtgg acgccgggga gaaagcgcgc cagcaggtcc
     2521 gcggtctcgc gggcctcgtc ggcggacacc gcgcgatcga ccgactgcca cgtggtgggc
     2581 gcgccgccgt agtggatggc cgccttgagc ccgcctccca cgtcggggaa tccgtacagc
     2641 aagcgctcgc cgtcgatttg aatcgcccac acttggttga gtgacgccgc gtcgcggccg
     2701 ttcgtgaacc agaacatcgg ttgccgcgtg accgtcgccg cgacccccgc gtccgccagc
     2761 cacgccgtca tgcccgcgcc cgcgcagaga atcaatcgag ctgctgggta gcgccccgat
     2821 ttcgtttcta cgaccacgcc atcgccgctc gcgcgccaag cgatcacgga ttcatccgcg
     2881 tggatgctgg cccccgcggc cacggccagc cgaaggtgcg cgtcgatgca ccgctcgggg
     2941 tgaaggagcc ccgcgcgcgc ctcgctcaac gcgaccatgt cgctcggcaa tcgaagccac
     3001 ggcgcgcgcg ccgcgagcgc ggcaccgtcg agccgctcga ccgcaatgcc gtgttcgcga
     3061 cccgcgcgct ccacccccgc gaccagctcc gcgtccggcg ggcccgcgaa gatcccgccc
     3121 gtttcgacga tgagctcggt tgccgcgtcg cgcgcgaggt cgtgccagag ggcgtatgcg
     3181 cggcgcagga gcgggacgta gcgcgcgtct tcgtagtagg cctcgcggat catgcgcgtc
     3241 tcgccgtgcg acgagcccag cgcgtgcggc ggcgtgtacg cgtcgaagcc caccacgcgc
     3301 gcgccgcggc gcgcgaggtg acacagcgcg gcgctgccca tggcacccaa gccgaccacg
     3361 gccgcgtcga acggcttcac gccgcgcccg cctgtgctat tatcgaggtc ccgaaaggag
     3421 tcgccatgcg gctgcgctcc gtgctgttcg tcatcgtgct ggtccttccg ctcgcgtcgg
     3481 cgcatgccga cgaggtcttc gtcgcgagtc tctcgggcgc ccgcgtgggc tcgccggcca
     3541 ccggcgctgc caccttcacc ctgcgcgcgg acctctccgc catcgactac gtcgttacct
     3601 acgacaacct atcgagcccc gagtacatgg cgcacgtcca ccgcgcggac gacaccgtct
     3661 tgatcttctt gccgctgggc acgcccaaga tcgggacgtg ggaggcaccc agcgcgcaag
     3721 acatcgccga gcttcgcaat gaggaattgt acgtgaacgt ccacagcgaa tactacccga
     3781 ccggagagat tcgcgggacg ctgcgccggc aggcgttgcc cgcggcggtc accacgtggg
     3841 gagcgatcaa ggcgctgtat cgctagactt cgagcagccg ggtgagattt ccgcccagca
     3901 ccatccgctg ctcgtcgtta ctgaagccgg ccgcgcgcat cgtctcttgc tgggcggtta
     3961 gcacgtcggc gcggtagccg cgcggaaaca ctcccgaatc cgacccaaag agcatacgat
     4021 cgacgccgaa ggagacgcgc gccgcctgga acacttcggc cagcgagagc ggcgggtcgt
     4081 gctccaagat ccaggcgttc gaccccgcgg tgtcggtgta gacgttggga cacgtcgcgc
     4141 ccagcgcgag cgcttcttcg aaccagcccg agccgaagtg cggcaccacg aagcgcacgt
     4201 cgggattggc gcgcgcgacc gggatcagat cgcgcggccg gctcctctcg gcggggaaat
     4261 cggcgttcag accgaccagc ttgcgcacgt tcacgcgcag cttgccgcag tggacgaaca
     4321 cgaccaagtg gtgccgcttc gcgacctcga tcaccttcgc cacttcgggc gacccgattg
     4381 agaactcgtg catggaggga aacagcagca cgcccttgaa gtggtacgtg ctctgcatgg
     4441 actccagggc ctgcagggta gccggcgcgg tcgggttgat gacggtgtag ggaaacaatc
     4501 gccgcgggtg gcttgcggcc gcctcgccca ccgacttcat ttcggtagga cacgaggcga
     4561 acaagaccgc gcggtcgatg cggttcttgt cgagctcggc gatccagcgc gcggcgtgca
     4621 ccttcggatc ggggccggga atctcgagac cgccggcgcg gacgcgcccg agcacggtct
     4681 cggggtcctc gcgcgggaca aaggtggcct ggaacgcgta gaactcgcgc ccgaagaagt
     4741 ggatatgggc gtcgaacacc attctagagc ctccgcggcg ggggtgcggt gctatagtcc
     4801 tgggcagtat gccccccggt tgggcgcggg gtcaatcaga cttggaaaat ggcggcacat
     4861 gcccaccgct gttgctatta gacagttcag acggacatga atcacacggt ttccacaaga
     4921 tattggcacc tcctcgacga cggccgggtc cagtgcgatg tctgcccgcg cttctgcaag
     4981 ctgcgggagg gccagcgcgg tatgtgcttc gtccgcgccc gcgagggcga cgccatagtt
     5041 ctaacgacgt acgggagttc gagcggcttc tgcatcgacc ccatcgaaaa gaagccgctc
     5101 aatcaccttt tgcccggttc cgcggtgctg tcgttcggga cggcggggtg caacctggcc
     5161 tgcaaattct gccagaactg ggatatttcc aagtcgcgtg aggtggaaac gctttccgag
     5221 gaagccaccc ccgaagcgat cgcgcgcgcg gcggtcgaga ccggctgccg cagcgtcgcc
     5281 ttcacctaca acgaccccgt cgtgttcatg gagtacgcaa tcgacgtcgc caaagcgtgc
     5341 cacgagcggg gtattctcac ggtggcggtg accgcgggcg aaatctgcga cgaaccgcgc
     5401 cgcgagttct atcgctacat ggacgcggcc aacgtcgact tgaagggctt caccgaggac
     5461 ttctactggc gcgtgtgcaa gggtgacttg aagacggtgc tggacacgct cgtgtatttg
     5521 aagcgcgaga ccagcgtgtg gttcgagatc acgaccctgc tgatccccgg cgagaacgat
     5581 tccagcgacg agatcgaggc cgagtgcgcg tggatccgcg agcacttggg gctcgacgta
     5641 ccgctccact tcaccgcctt ccaccccgac ttcatgatgc tcgacaagcc gcccaccccg
     5701 cccgagacct tgacgcgcgc gcgcgacatc gcgctcaagc aagggctgcg ctacgtctac
     5761 gtcgggaacg tccacgacga atccgggagc agcacctact gcccgaaatg cggcacgacg
     5821 ctcatcggcc gcgactggta tcgattgacg gcctggaacc tcgactcgcg cgggtgctgc
     5881 aagaaatgtg gaacgcagat ccccgggcgc ttcgaagcca aaccgggcac ctggggcgcg
     5941 cgccgcgagg cggtcaacat gcgggcgttc gcgtgatcgc ctcgcgttga tttctcctcc
     6001 caaaccgcct acaatgccgg tatcacgcgg gccggccgga cccccgctca agcgaacaat
     6061 accgacgaca tttccaggag gcacatcctc taatgaaacc gcctttcaag ctgctcggct
     6121 tcgaccatgt gacgtttgcg gtcgggaatg ccaagcaagc ggctgtctat tatcagtcgg
     6181 tcttcggctt cgacctcgtg gggtatcgcg ggttggagca gggagaccgc cagaccgcga
     6241 gctacgtcct gcgccagaac cacgtcacct tcgttctcaa tacgccgtac acgcccagtt
     6301 cgtcgctcaa caacctgctt gcgaagcatg gggatggtgc gactgacatc gcgttcgcgg
     6361 tggacgacgc ggtcaaggcg tgggagcaca ccaccaagca cggggcgaag aactggttcg
     6421 agccgcgcac gatggaagac gaaggcggca aggtgattct ctccggcatt cacacctacg
     6481 gcgacgtgac ccacaccttc gtgcagcgcg agggatacaa aggcgtgttc ctccccggct
     6541 acgcgccggc gaagtcgcgc ggcgaagtca ggcaaacggg ccttttgttc atcgaccata
     6601 tcgtcggcaa ccagcccgac agcgagatgg aagacgtggt tcgcttctac gagaacatct
     6661 tcggcttcca cagactatgg acggtcgacg acaaggacat atcgaccgag tacacggcac
     6721 tgcgctcggt ggtggtcgcg gacgacaacg agaacgtcaa gatgcccatc aacgagcccg
     6781 cgtcggggct gaagaagtcg cagatccagg aatacgtcga gttcaactcc ggagccggcg
     6841 tgcagcacat cgcgatgtcg accaacgaca tcatcgggac catcaagcag ctcaccgcca
     6901 atggcgccga gttcttgacc gtgccgggct cgtactacga cgagctgccc aagcgcgtgg
     6961 gcgacatcga cgaagagatt ccgaccctgc gcaagctggg catcctggtc gaccgcgacg
     7021 acagcggcta cctgctgcag ttgttcacca agccggtgca ggaccgcccg acgctgtttt
     7081 tcgagttgat ccagcgcaag aacgcgcgct cgttcgggaa gggaaacttc aaggcgctct
     7141 tcgagtcgat cgagcgcgag caagcgcgcc gcgggacgct ataaggagtc agtactatgc
     7201 cgatctacat ccagcgaggc aagattccgc accggcggca caccgtcttc aagagcccgc
     7261 aagggaagca ctactacgaa gagcacatca gccgcctcgg tttctcggac atctattcca
     7321 acgtctacca cgtgcacatg ccgacgcgtg tcgcgaaagt ggggaagttc cgccccgtca
     7381 ccctcgagcc gctcgaggcc gagcatcgtc atcgtcattt gaagaccttc gacctgaagc
     7441 cggagggcaa ctggctgacc ggccgccgcc ccatgatgtt caacgccgac gtgatcctcg
     7501 gcaccacgag tcccgtgcgc ggcgcgagcg atttcttcta caagaacgcc acctgcgacg
     7561 aggtgcactt cgtccacaag gggcgcggcg ttctggatag catgttcggt cgcctcgcgt
     7621 tcggcgaggg cgattacgtc gtcattccgc gcggcgtgat tcacaagatg acgttcgaga
     7681 gcgacgacaa ccgcgtgttc ttcgtcgaat cgtcgggccc cgtcgactgg cccaagtcgt
     7741 accgcaacca gtacggccag ctgctcgagg acgcgcccta ctgcgagcgc gatattcgta
     7801 cccccgagtt cgtggaggcg atggacaagc cgggcgagtt cccgatgcac atccggctgc
     7861 gcgacggcat ccaggaatac ctgtggggcc agcacccgtt cgacctggtg ggctacgacg
     7921 gcttctacta tccgtggatt ttcaatatca aggactacat gtcgaaggtg gggcgcgtgc
     7981 atttgccgcc cccgacgcac ctcaccttca ccggtccggg ctacgtgctg tgctcgttct
     8041 gcccgcgtcc cttcgacttc gaagagggtg ccgtgccgat cccgtacgcg cactccaacg
     8101 tggacagcga cgaagtgatc tactacgtcg acgggagctt catgagccgc aagggcatcg
     8161 gcgtgggatc gatcacgctg cacccgtacg gcattccgca cggtccgcag ccggggttgc
     8221 tcgagcagtc gctgggtgcc aaggaaaccg gcgagcttgc ggtcatggtg gacaccttcc
     8281 gcccgctcaa ggtggcgaag gtgtgtgcca ccatcgatga cgccaactac ccgtacagct
     8341 ggcagtacta atgcgcgcgt cgcgacgcga agcggcagca tgatcatcga cccggccacg
     8401 actcgcaagc aagagttcta cccctggctg atccggtgca tcgtgccgcg cccgatcgcg
     8461 tgggtgtcga ccatctcgcg cgagggcgtc cccaacctgg cccccttctc gttcttcacc
     8521 gccatctcca ccgaaccgcc caccctgtgt ttcgcccccg gccggcacgc cgtgacgggg
     8581 aagaagaagg acacgctggt caacatcgaa gcgaccggcg acttcgtggt caacgtggta
     8641 ccggaagagc tggccgaggc gatgaacgag actgccaccg actaccccca cggtgtgagt
     8701 gagttcgcgg aggcgaggct caccccggtg ccggccgaac gggtgaaggc cccgcgcctg
     8761 gcggagtcgc ccatccactt cgaatgcgag cgctacgaga tcgtccaggt cgggcccgac
     8821 ggcgtgggcg gcggggcgct cgtgatcggc aaaattgtcc tactccatgt cgacgaccgg
     8881 gtcatcaccg acggcaaggt cgactacgac ttgttccgcg cgctcgggcg cttgggcggc
     8941 atggagtaca cacgcacaca cgaccgcttt cagatggccc gcaagaagta cacgccgcgc
     9001 tcgtagcgac gcgcgcatta ctcctcatcg ttgcgaattg catcgcgtcg tttcgacgtc
     9061 gcgtcgcgct ccttatatag ggcgatcgca cgcgccacgc cgtttctcgg gcaactattt
     9121 cccacgattt ggccccgtgc gagccctccg cagtttgcat tagccgatgc gcgcgatttc
     9181 acccggcggt cttccagaag tggtcgcaga ttcttccaag ggggccggaa cgggcgaaaa
     9241 gcgtatcccg cgacgtggca catggattgc agcggtggga ccgggggtaa tctatgtgta
     9301 agtcgcgcaa cagactgagc caagcaggct acacgctcat ggagctcatg atcgtcgtcg
     9361 cggtggtcgg catactggcc gccatcgcgg tgccttcgtt ctcgggctac ctcaaacgaa
     9421 cgcgtgtggt cggagcgcgg aatcagctga tcaccgactg ttacctcgct cgctcgcttg
     9481 ccatctcgcg ccgcgaaacc atcaccatgg tattcagcgc ggatcagtat cagatcgtca
     9541 acggcggtgg gaccgtattc cggaccacgc cggcgcaaag cggcgtgacc ttcaacgcga
     9601 gcgacgaccc caacttttat gcgtggggat tggccgacgc cgtcgacatc gcgatcgatg
     9661 gtccgtccaa ctcgacggac gtcagccttt cacccacggg agttgccagc catggttact
     9721 aaacgcgtgg cgaacgcgca ccatcgccaa gaaggcttca gcctcgtcga gttgatggtg
     9781 gccctggtca tcttgaccat cggactgctg ccgctcgcat tcgtgcagac gcgcgcgcag
     9841 caagacgtgt tcgactcggg ccgctacacc gaggcgatca cgatcgccac tctgcagatg
     9901 gaagagacca agagtctggg attcggcgtc gccgtaaccg acaccggtac ggtcgacaac
     9961 tacacgtggg tgcgcgacgt tcaaagcatt agtccgacac tcgaccagat cacggttagc
    10021 gtgtcgtgga ccgagcgcgg aaccccgcgg ctcgtccaga tcatcaaccg actctcggac
    10081 cggtaatacc atgagcaagc atgcacggaa gattcgcaac gcgttctcgg acaaccgggg
    10141 catcaccttg gtcgagctcg tcgttaccgt gatcatcctc gggatcgtcc tgctcgtcgt
    10201 caaccaggtg ttcttctcga ccacgcgcat ctacggcagc acgtcgacgc gcgcgaacca
    10261 gcagatgaac gcgcgcgcgg gcctctcggt catggtcacc gagctgcgtc cggccggatc
    10321 cgaccccgcc gagcagggca tccagggggt cttggccgcg cagagcgaca cggtgcacgt
    10381 ccaggccgac ctgacgggtg acggggccat ccagaccgcg gagccgtccg aggacatcat
    10441 gtactactac gactccaacg cccaagccgt catgcgcgag atcg
//
LOCUS       DASPGK010000088         5573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1310295, whole genome shotgun sequence.
ACCESSION   DASPGK010000088 DASPGK010000000
VERSION     DASPGK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310295"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VEC56_02435"
     CDS             <1..442
                     /locus_tag="VEC56_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV13042.1"
                     /translation="EAHEIENDELASYFGAKEGILVLGVEDESVAAKAGVKAGDVIQK
                     IGDEKVTDIGDLRDAVRDFDEGDEFTISVLRHGKSQSLKATMDEQEFSFFRGEAPMFR
                     EWRHTPRAPRAPRVHVDRESLRDEIDDLKREIREMKEQLEREDG"
     gene            complement(491..1618)
                     /locus_tag="VEC56_02440"
     CDS             complement(491..1618)
                     /locus_tag="VEC56_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13043.1"
                     /translation="MAPDLPTVGFVVDDLGHGGAQKQLVLLAEALRPRFATRVYALSP
                     VDAPHGPRLRARGVPVTAFPRRSGLDFERLQGLTRALAADRVDVVHGFLDASDIYAFL
                     AGRRIGKPVVLSLQSDRLQLKGARGRVLRWMHRHADAVTVNSEAGRDYLVERVRVARD
                     SVHVVRNIADVFAGPRGGEPTPPVVGCVGRLVALKRFDAAVRALPEVRAQVPGTRLEL
                     VGDGPAREELRALSASLGVGDAVAFAGAVDDATPLIARMSCLVLPSAFEGLPNAALEA
                     MGCGIPVVASPVGDLASIIVDGSTGVVVRDTAPATLAAGIVRALTDRSLRERSRQDGP
                     RAIRERFSPEVPLSVLIPLYDRLCKRTGAAALKATTPVLGE"
     gene            1755..2921
                     /locus_tag="VEC56_02445"
     CDS             1755..2921
                     /locus_tag="VEC56_02445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13044.1"
                     /translation="MNVVWFAEIKWEYLRTRKQQIVLRRPHGVDVVFFEPYVKGRENR
                     FDLRSVDGIRVATVPFVKSIPGRGPLRALLDRAPARRAVDAIAVRRVRAHLDAAGVRP
                     AESVFVFSNIYALPVARALEPRRLVYDCNDAHGDFPGAPEWARAYRDDAFRAADAVVV
                     SAARLRDDAVRVRGSHARVEVIGNGVDFAAFRRAADAARGSASTDDTVRVGYLGAIAP
                     WLDFELIAAAARARPRWSFDLVGPVIGGVEMDLAALLTLGNVRHRPAVAHERVPEILL
                     DFTVGVIPFRRTPLTAGVNPNKLYEYLAVGLPAVATPFSPETAHYAGQADDADTVVAI
                     ANDANELVKACEAFAAARRDPAARARISERAVAIAAAHDWKTIAAQFWRVIGNV"
     gene            2991..3668
                     /locus_tag="VEC56_02450"
     CDS             2991..3668
                     /locus_tag="VEC56_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HXV13045.1"
                     /translation="MDFLIRLGWIFTSPGRVFDDIRERRVSWVQPWLISSAIYALITW
                     IAMPIQRAILEVHPDLTTEQIDQQIEMMDSFGFMWVILAPAGALIITFVMAGLSYIAV
                     TMASRAANFKQYLTLSFYTGIVGMMGQLVSALVIRARGLENITGPEDGQMALSLRAIA
                     PDNAALRGLYGSIEFFAIWSLVLVAMGLMRIFGMSRGASIAVAVVLWVLYAGMMVIAE
                     VLGGMAG"
     gene            3799..>5573
                     /locus_tag="VEC56_02455"
     CDS             3799..>5573
                     /locus_tag="VEC56_02455"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV13046.1"
                     /translation="MKQFPAAQIRNVALVGHQESGKTMLSESLLYTAGAIPRLGRIED
                     KNTTMDYDSEEIARGISIHSGVAHLEHAGTKVNLIDTPGYEDFVGDVLLGLDVVEGAI
                     VCLRADAGVEVGTDRVWGFLQDRGLPALFVANRMDKEHADYDRCLALLHEHFGGGVHA
                     LQVPIGAGDKFRGVVDLIDMKAYEFERDGKGAAKPIDIPADLKEKASALRKELMESAA
                     ESDEKLMEKYLETESLSEEEFRAGLAAGVRTAQIFPVLCASAAHNMGAALVLDSIVRL
                     LPGADAGKCVTVDDKVIKTEANGAPLAYVFKNISDPHVGDMLCVRVFHGVLAPALDVH
                     NTTRNNAERLGGLFVLEGKNRQDADKLQAGDIGALVKLKVTKVRDTLGDKASGTVVRP
                     TALPKPSINSAILPVAKGDDEKMGSGLHRIMDEDPTFEVTPSAETHQTIIAGQGELHL
                     EIIVHRLKERFGVNVQLEKPRIPYRETIRRPAEAQGRHKKQTGGRGQFGDVWLKIEPL
                     QRGKQFEFVDGVVGGVVPGKFIPAVEKGVVATMEMGVIAGYPMVDVRATIYDGSYHTV
                     DSSEQAFKTAGSLAFKAAVEKANPV"
BASE COUNT          948 a         1848 c         1936 g          841 t
ORIGIN      
        1 cgaggcccac gagatcgaga acgacgagct ggcctcgtac ttcggcgcca aggaaggcat
       61 cctggtgctg ggggtcgaag acgagtcggt cgccgccaag gcgggggtca aggccggcga
      121 cgtcatccag aagatcggcg acgagaaggt caccgacatc ggcgacctgc gcgacgccgt
      181 ccgcgacttc gacgagggcg acgagttcac gatcagcgtg ctgcgccacg gcaagtcgca
      241 atcgctcaag gcgacgatgg acgagcagga gttctcgttc ttccgcggcg aggcgccgat
      301 gttccgcgag tggcgtcaca cgccgcgcgc gccccgagcc ccgcgcgtgc acgtcgatcg
      361 cgaatcgttg cgcgacgaga tcgacgatct caagagagag attcgggaga tgaaggagca
      421 gctcgagcgc gaggacggct aacgcctccg cccggcaggt gcgtaaacgc gaaacccaag
      481 acacgcattc ctattctcca aggacgggcg tcgtcgcctt caaggcggcg gcgcccgttc
      541 gcttgcacag gcggtcgtac agcgggatga gcacggaaag gggaacctcg ggcgagaagc
      601 gctcgcgaat cgcgcgcgga ccgtcttggc gggaccgctc gcgcagcgat cgatcggtga
      661 gcgcacgcac gatgccggcg gcgagggtgg cgggcgcagt atcgcgaacc accacaccgg
      721 tcgacccgtc gacgatgatc gacgccaggt ctcccaccgg cgaggcgacc accgggatcc
      781 cgcaccccat cgcctcgagc gcggcgttgg gaagcccctc gaaggcggac ggcagcacga
      841 ggcacgacat gcgcgcgatg agcggggtcg cgtcgtccac ggcacccgcg aaggcgacgg
      901 cgtcgccgac gccgaggctc gcactcaacg cgcgcagctc ctcgcgggcg ggtccgtctc
      961 ccaccaactc gagccgcgtt ccgggcacct gtgcgcgcac ctccggcagc gcgcgtaccg
     1021 ccgcgtcgaa gcgcttgagc gcaaccagac gtcccacgca tccgaccacg ggcggggtgg
     1081 gctcgccgcc gcgcgggccg gcgaagacgt cggcgatgtt gcgaacgacg tgcacgctat
     1141 cgcgcgcgac gcgcactcgt tcgacgaggt agtctcgtcc cgcctccgag ttcaccgtca
     1201 ccgcgtcggc atgacggtgc atccagcgca gcacccgtcc ccgcgcgccc ttgagctgga
     1261 gtcggtcgct ctgcagcgag agcacgaccg gcttcccgat ccggcggccg gccaggaacg
     1321 cgtagatgtc cgatgcgtcg aggaatccgt gcacgacgtc gacgcggtcg gcggcgagcg
     1381 cccgcgtcag cccttgcaga cgttcgaagt cgagccccga gcggcgggga aacgccgtca
     1441 ccggaacgcc gcgtgcgcgc agccggggcc cgtgcggcgc gtcgaccggg gagagagcat
     1501 acacgcgggt tgcaaagcgc gggcgcagcg cctcggccaa gagcacgagc tgtttctgcg
     1561 cgccgccgtg accgagatca tcgacgacga agccaacggt gggaagatcg ggcgccatgt
     1621 cgcgattcta gaggaacacg cggcgtgccg accacatatc tgttgggacc gcccgggccc
     1681 cgtgatagag tgcgaccacg tgcgcgccgc gggtgtgtcg tggcggcagc cgcacgggat
     1741 ggcccgcaca acgcatgaac gtcgtttggt tcgccgagat caagtgggag tacctgcgca
     1801 cgcgcaagca gcaaatcgtg ctgcggcgcc cgcacggcgt cgacgtcgtc ttcttcgagc
     1861 cctacgtcaa ggggcgcgag aaccgcttcg atctgcgttc cgtcgacggg atccgtgtcg
     1921 ccaccgtacc gttcgtgaag tccattcccg ggcgcggccc cctgcgcgcg ctcctcgacc
     1981 gcgcgccggc acggcgagcg gtcgacgcga tcgcggtgcg gcgcgtgcgc gcacacctcg
     2041 acgccgccgg cgtccggccg gccgagagcg ttttcgtatt ctcgaacata tatgccttgc
     2101 cggtcgcgcg cgctctcgag ccgcggcgac tcgtgtacga ctgcaacgac gcccacggcg
     2161 actttccggg cgcgcccgaa tgggcgcgcg cctaccgcga cgacgcgttc cgtgccgcgg
     2221 acgcggtggt ggtgagtgcc gcgcgcctgc gcgacgatgc ggtacgcgtg cgcggctcgc
     2281 acgcgcgcgt ggaagtgatc ggtaacggcg tcgacttcgc ggcctttcgt cgcgccgccg
     2341 acgccgcgcg cgggtccgcc tccaccgacg acacggtgcg cgtcggctac ttgggggcga
     2401 tcgcgccgtg gctcgacttc gagttgatcg ccgcggccgc gcgcgcccgt ccgcgctgga
     2461 gtttcgatct ggtggggccc gtgatcgggg gcgtcgagat ggacctcgcc gcactgttga
     2521 cgctgggcaa cgtgcgccac cgtcccgcgg tcgcgcacga gcgcgtcccc gagatcctcc
     2581 tcgacttcac cgtgggcgtg attccgtttc gacgcacccc gctcacggcg ggcgtcaatc
     2641 ccaacaagct ctacgagtac ctggcggtgg ggctgccggc ggtcgcgaca ccgttctcac
     2701 cggagaccgc gcactacgcg gggcaggcgg acgacgccga taccgtggtc gcgatcgcga
     2761 acgacgccaa cgagctggtc aaggcctgcg aagcgttcgc ggccgcgcgc cgcgaccccg
     2821 ccgcgcgcgc ccgtatcagc gagcgcgcgg tcgccatcgc cgccgcgcac gactggaaga
     2881 cgatcgcggc ccagttctgg cgcgtgatcg gcaatgtctg acacgcgagt gtgctacact
     2941 gcgcgggccg cgcgcgcgcg gtcgatggtg tcgacaacga aggaggcgtc atggacttct
     3001 tgatacgcct gggctggatc ttcacgtccc ccggtcgcgt gttcgacgat attcgcgagc
     3061 gccgcgtgag ctgggtgcag ccgtggctaa tctcgtcggc gatctacgcg ctcattacct
     3121 ggatcgcgat gccgatccag cgcgcgatcc tcgaggtgca tcccgacctc accaccgagc
     3181 agatcgacca gcagatcgag atgatggaca gcttcggatt catgtgggtc atcctcgccc
     3241 ccgcgggcgc gctgatcatc acgttcgtca tggcggggct ctcgtacatc gcggtcacga
     3301 tggcctcgcg ggccgccaac ttcaagcagt acctcaccct gtcgttctat accggcatcg
     3361 tgggcatgat ggggcagctg gtctcggcgc tcgtcattcg agcgcgcggg ctggaaaaca
     3421 tcacgggacc ggaggacgga cagatggcgc tgagcctgcg cgccatcgcg cccgacaacg
     3481 ccgcgttgcg cggactgtac gggagcatcg agttcttcgc catctggagc ctggtgctgg
     3541 tggcgatggg gctgatgcgg atcttcggga tgtcgcgcgg ggcgtcgatc gcggtggcgg
     3601 tggtgctgtg ggtcctgtac gccggcatga tggtcatcgc cgaggtgctc ggcggtatgg
     3661 ccggctgacg gtcgcgcggc tccgcaccga atttgacacc gccctcgccg cggtgataga
     3721 atccaaaacc caaccccaag tctacgttga ggttgcagcc gttccctcga gacgattcgg
     3781 aataggagga ggctcatcgt gaagcagttt ccggcggccc agattcgcaa cgtcgcactg
     3841 gtcggacacc aggaatccgg caagaccatg ctttccgagt ccctgctgta cacggcgggt
     3901 gccattccgc gcctgggccg catcgaggac aaaaacacca cgatggacta cgactccgaa
     3961 gagatcgcgc gcggcatctc gatccactcc ggtgtcgcgc acctcgagca cgcgggcacc
     4021 aaggtcaact tgatcgacac gcccggctac gaggatttcg tcggcgacgt gctgttgggc
     4081 ctcgacgtgg tcgagggggc catcgtgtgc ctgcgcgccg acgccggcgt ggaagtcgga
     4141 accgaccgcg tgtgggggtt cctccaggat cgcggcttgc ccgcgctgtt cgtggccaac
     4201 cgcatggaca aggaacacgc cgactacgac cggtgcctcg ccctcttgca cgagcacttc
     4261 ggcgggggcg tgcacgcgct gcaggtcccg atcggggccg gcgacaagtt ccgcggggtc
     4321 gtcgacctga tcgacatgaa ggcgtacgag ttcgaacggg acggcaaggg ggccgcgaaa
     4381 ccgatcgaca tccccgccga cttgaaggaa aaggcgagcg cgctgcgcaa agaattgatg
     4441 gaatcggcgg ccgagtccga cgagaaattg atggagaagt acctcgaaac cgaatccctg
     4501 tccgaggaag aattccgtgc cggactggcg gccggggtgc gcacggcgca gatctttccc
     4561 gtcctgtgcg cgtcggcggc gcacaacatg ggagcggcac tcgtcctcga ttccatcgtt
     4621 cgcttgctgc cgggcgcgga tgccggcaag tgcgtcaccg ttgacgacaa ggtgatcaag
     4681 accgaagcca acggcgcgcc gctggcgtac gtgttcaaga acatctccga cccgcacgtg
     4741 ggtgacatgt tgtgcgtgcg cgtcttccac ggcgtgttgg cgccggccct cgacgtgcac
     4801 aacacgaccc gcaataacgc cgagcggctg ggcggtctgt tcgtgctcga aggaaagaac
     4861 cgccaggacg ccgacaagct gcaggcgggc gacatcgggg cgctggtgaa gctcaaggtg
     4921 accaaggtgc gcgacacgct gggtgacaag gccagcggaa ccgtcgttcg tccgacggcg
     4981 ttgcccaagc cgtccatcaa ctcggccatt ctcccggttg caaagggcga cgacgagaag
     5041 atggggagcg gcctccatcg tatcatggac gaggacccga ccttcgaagt cacgccgagt
     5101 gcggagacgc accagaccat catcgccgga cagggcgagt tgcacctgga gatcatcgtg
     5161 caccggctca aggagcgctt cggcgtcaac gttcagctcg agaagccgcg cattccctac
     5221 cgcgagacca ttcgacgccc ggcggaagcg caggggcgtc acaagaagca gaccggcggc
     5281 cgcgggcagt tcggcgacgt gtggctcaag atcgagccgc tccagcgcgg caagcagttc
     5341 gagttcgtcg acggcgtggt gggcggcgtg gtgccgggca agttcatccc cgcggtcgaa
     5401 aagggcgtcg tcgccaccat ggagatgggg gtgatcgcgg gttacccgat ggtcgacgtc
     5461 agggcgacca tctacgacgg cagctaccac accgtcgatt cgtccgagca ggcgttcaag
     5521 accgcgggct cgctcgcgtt caaggccgcg gtcgagaagg cgaacccggt gtt
//
LOCUS       DASPGK010000089         4326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_490988, whole genome shotgun sequence.
ACCESSION   DASPGK010000089 DASPGK010000000
VERSION     DASPGK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490988"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VEC56_02460"
     CDS             complement(<1..588)
                     /locus_tag="VEC56_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV13047.1"
                     /translation="MKCAEVEKLLDDFVDGNLAPDRAQAVREHTASCLECAAELVALE
                     ALRETTTELSRDIEPLEDLWQEIEAGLFCRVEYGREGRTRVYGWVWRGPKWNAAGYAW
                     RLTAAAAIVLLVVAGVREWRTRGLANGAGWEVTTLQGAPVVGTHDLSDKGTLRPGEWL
                     ETDAASRARIDVADIGHVTVGPASALRLKGSSEKEH"
     gene            complement(585..1160)
                     /locus_tag="VEC56_02465"
     CDS             complement(585..1160)
                     /locus_tag="VEC56_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV13048.1"
                     /translation="MHAATQETTVMTPPEAIEIAAWVRLARTGDRAAYERLYRAHVGR
                     VHALCLRLSGERTRASELTQDAFVQAWQKLSGLESDAAFASWLYRLTVNMVLMRLRRD
                     KKWSAREAGSDVLEAVEPVRGRRDDGAGARVDLERAIAALPSQARVVFVLHDVEGYRH
                     EEIANHMNIAVGTSKAQLHRARRLLREVLDR"
     gene            1347..1727
                     /locus_tag="VEC56_02470"
     CDS             1347..1727
                     /locus_tag="VEC56_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXV13049.1"
                     /translation="MRGFLIRALISALGLWVASAILPSVHSTGFLALIGAGLLLGIVN
                     AVIRPLIVVMTLPVTIVTLGLFILVINAAMIGLVAKMLNGFAVGGFGGAVMTAIIVSI
                     VSWCANAFVGTTGRFERFAGGRKA"
     gene            1744..2511
                     /locus_tag="VEC56_02475"
     CDS             1744..2511
                     /locus_tag="VEC56_02475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV13050.1"
                     /translation="MTERHELNPQTRFSNRVDDYIRYRPGYPRELMAWLREAIGVSPA
                     WRVADIGSGTGLLSQHFLEAGCTVYGLEPNAAMRAAAEREFADNPHFVSVAGSAEATT
                     LPDASVDLVGAGQAFHWFEPVATRGEWRRILKPDGWAMVVFNSRLIEATPFMRAYDQY
                     LVDHAVDYTGVDHRRGLHEKLRAVLGEYRERRFRFTLRHRYDDVRGLSMSSSYVPAPG
                     HPKHEAFFAGLRETFDAHAIDGVVEFPYETEAYVGRV"
     gene            complement(2508..3596)
                     /locus_tag="VEC56_02480"
     CDS             complement(2508..3596)
                     /locus_tag="VEC56_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13051.1"
                     /translation="MSKAARHTYALAVVLSLSGFVACSDDSTAPPPDDGPTRTYRMGF
                     SGIPPRPDLPTAIAAIDMWSLRADAAIMSFELPWDSLLAGVPVETLVIREQESLANYY
                     RFKGHELWIYLDPANGLNRGGEADALVRNGRSITEPAIQQMFRRYAVVVDSIVRPEHL
                     GLTLETNLIRGASPDSLYQAIRQVANDAAADVRAIDATVKLSVSVQVDFAWGRFGGPY
                     QGVDQDFVDFPFIEELGLSSYPYLARFAQPEEIPIDYYTRLIEGRTIPVMVTEGGWSS
                     ATIDTIVSSEDEQRRYIERQARLLDEANAIAVFQLTFTDLDLASFPPQPPNSILYLFV
                     TCGLVDADLNPKAALTAWDAIFARPLGS"
     gene            3713..>4326
                     /locus_tag="VEC56_02485"
     CDS             3713..>4326
                     /locus_tag="VEC56_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV13052.1"
                     /translation="MSFHMLNLPEPILRALDDAGYQTPTEIQAQAIPPALEGRDLIGR
                     AQTGTGKTAAFAIPILVRLSKTPAHAGPPSREEPDDDAEASKPHARPPHHPHRKSHDA
                     PARAVRSLILTPTRELAVQIEESFKAYGKYVDVRALAVYGGVRIDRQLKKLRSGVDVV
                     VATPGRLLDHLGRHTVDLRHVEIFVLDEVDRMFDMGFIQDVRKI"
BASE COUNT          716 a         1470 c         1362 g          778 t
ORIGIN      
        1 atgctccttt tcgctcgagc ccttgagtcg aagcgcactc gcggggccga cggtcacgtg
       61 gccgatgtcg gcgacgtcga tgcgcgcgcg ggaggcggcg tcggtctcga gccactcgcc
      121 ggggcggagc gttcctttgt cagacaggtc gtgggtaccg accacgggcg cgccttgcag
      181 cgtcgtcact tcccagcccg cgccgttcgc aagaccgcgc gtgcgccatt cgcgcactcc
      241 ggcgacaacc aacagcacga tggccgcggc ggcggtgaga cgccacgcgt agccggccgc
      301 gttccacttc gggccgcgcc acacccaacc gtacacgcgg gtacgtcctt ctctcccgta
      361 ttcgactctg cagaacagcc cggcttcgat ctcttgccac agatcctcga gcggttcgat
      421 atcgcgcgag agttccgtag tcgtctcgcg cagcgcttcg agcgcgacca actccgcggc
      481 gcattccagg cacgacgccg tgtgctcgcg aaccgcctgc gccctgtcag gcgccagatt
      541 cccgtcgacg aaatcgtcca gcagcttctc tacttcggcg cacttcatcg atccaacacc
      601 tcacgcagca atcggcgcgc tcgatggagc tgcgccttgg atgttcccac cgcgatgttc
      661 atgtgatttg cgatctcctc gtggcggtag ccttcgacgt cgtggaggac gaacacgacg
      721 cgcgcctgcg aagggagcgc ggcgatagcc cgctccaaat cgacgcgcgc gcccgcaccg
      781 tcgtcgcgac ggccgcgcac cggctcgacc gcctcgagta catctgagcc cgcttcgcgg
      841 gccgaccatt tcttgtctcg tcgcagtctc atgagcacca tgttcaccgt cagtcgatag
      901 agccaggacg cgaatgccgc gtcgctttcc aagcccgaga gtttctgcca cgcctgcacg
      961 aaggcgtcct gggtcagctc gctcgctcgc gttcgctcgc ccgagagtcg caggcagagc
     1021 gcgtgtacgc gcccgacgtg ggcgcggtac aaccgctcgt acgccgcacg gtcacccgtg
     1081 cgcgcgaggc gcacccaggc cgcgatctcg atcgcttccg gcggggtcat caccgtcgtc
     1141 tcctgggttg ccgcgtgcat gcgtgaattg cgatctcctt cgcccgttta gatgccatgg
     1201 tgctccgaaa ggttggagtg ccggaccggc aaattctgcc ccgcttcccc aatccccttt
     1261 cgagctatat aatgaggcac ctcggcccgc cgcgcacgcc tttgcgcccg gcgggtcgaa
     1321 ggccccactc ttgaggaggg tgatcgatga gaggattctt gattcgcgcg ctcatttccg
     1381 cgctcggact gtgggtcgca tcggcgattc tccccagcgt gcactcgacc ggttttctcg
     1441 cactgatcgg cgccggactc ctgctcggca tcgtcaacgc cgtgatccgg ccgctcattg
     1501 tagtaatgac gctccccgtc accatcgtca ccctcggtct cttcatcttg gtcatcaacg
     1561 ccgccatgat cgggctggtt gcgaagatgc tgaacggctt cgcggtgggc ggattcggcg
     1621 gcgctgtgat gaccgccatc atcgtgtcga tcgtgagctg gtgcgcgaac gccttcgtcg
     1681 gcacgaccgg gcgcttcgag cgcttcgcgg gcgggcgcaa ggcgtaaccg cgcgcgcgcg
     1741 accgtgaccg agcgccacga gctcaatccc cagacgcggt tctccaaccg cgtcgacgat
     1801 tacattcgct atcgaccggg gtatccgcgc gagttaatgg cgtggctccg cgaagcaatc
     1861 ggggtctcgc cggcatggcg cgtcgccgat atcgggagcg gcaccgggct cctctcccag
     1921 cattttctcg aagcaggctg caccgtctac ggtttggaac ccaacgccgc catgcgcgcg
     1981 gccgcggagc gggaattcgc agacaatcca cacttcgtct ccgtggcggg atccgcggag
     2041 gcaaccacgc tgccggatgc gtcggtcgac ttggtcggcg cgggccaagc gttccattgg
     2101 ttcgaacccg tcgcgacgcg gggcgaatgg cgtcgcatcc tgaagccgga cggctgggcg
     2161 atggtggtct tcaactcgcg cctcatcgaa gcgacaccct tcatgcgcgc ctacgaccag
     2221 tacctcgtcg accacgccgt cgactacacc ggcgtcgatc accggcgggg gttgcacgag
     2281 aagctgcgcg cggtcctcgg cgagtaccgc gagcgtcgtt ttcgcttcac gctccgccac
     2341 cgctacgacg acgtgcgcgg cctctcgatg tcgtcgtcgt acgtccccgc accgggacac
     2401 cccaagcacg aggcgttctt cgccgggctg cgcgagacct tcgacgcaca cgcgatcgac
     2461 ggcgtggtgg agtttccgta cgagacggag gcgtatgtgg ggagggtcta gctccccaac
     2521 gggcgcgcga agatcgcgtc ccacgcggtg agcgccgcct tcggattcaa gtcggcgtcg
     2581 accaggccgc aggtcacgaa caagtagaga atcgaattcg gcggttgcgg cgggaacgag
     2641 gccagatcga ggtccgtgaa cgtgagttgg aacaccgcga tcgcgttcgc ttcgtcgagc
     2701 aaccgcgcct ggcgctcgat gtagcggcgc tgctcgtctt cgctcgacac gatggtgtcg
     2761 atggtcgccg agctccaccc gccttcggtc accattaccg gaatcgtccg tccttcgatc
     2821 aaacgcgtgt agtagtcgat cgggatctct tccggttggg cgaagcgggc caaatacgga
     2881 tacgacgaga gcccgagttc ttcgatgaag ggaaagtcca cgaagtcctg gtcgactccc
     2941 tgatacggac cgccgaagcg accccacgcg aagtcgacct gaacgctcac gctcaacttg
     3001 acggtggcgt cgatcgcgcg cacgtcggcg gccgcgtcgt tggcgacctg gcgtatcgct
     3061 tggtagagcg aatcgggtga agcgccgcga atcaggttgg tctcgagtgt gagccccagg
     3121 tgctccgggc gcacgatgct gtccaccacc accgcgtagc ggcgaaacat ctgttgaatc
     3181 gcgggctcgg tgatgctgcg gccgttgcgt accaacgcgt ccgcctcgcc gccgcgattg
     3241 agtccgttgg cgggatcgag atagatccat agctcgtgtc ctttgaaccg atagtagttc
     3301 gcgagtgatt cctgctcgcg aatcaccagc gtctccacgg gaactcccgc gagcagcgag
     3361 tcccacggca gctcgaagct catgatggcg gcgtcggcgc gcagcgacca catgtcgatg
     3421 gccgcgatgg cggtggggag atcggggcgg ggcggaatgc cggagaagcc catgcgatac
     3481 gtgcgtgtcg gtccgtcgtc tggcggtggc gcggtggagt cgtcggagca cgcgacgaac
     3541 cccgacaacg agagcacgac cgcgagcgcg taagtgtgac gagccgcctt cgacataggt
     3601 ccgtctatgg gcctcggcgt gcaaaccgcg cgccaaacag tgctttgtat cccctataag
     3661 ttcgttgtat gctacgggtg tatcgactgg cgctccaagc caagatatca ctatgtcatt
     3721 tcacatgctc aaccttccgg agccgatcct ccgcgccctc gacgacgcgg ggtaccagac
     3781 tcccaccgag attcaggccc aagcgattcc gcccgcgctc gagggtcgcg acttgatcgg
     3841 tcgcgcccag accggcaccg gcaagaccgc ggccttcgcc attcccatcc tggtgcgcct
     3901 gtcgaagacg cccgcgcacg ccgggccgcc ctcgcgagag gagcccgacg acgacgcgga
     3961 ggcgtccaaa ccccacgcgc gcccgcccca ccacccgcat cgcaagagcc atgacgctcc
     4021 cgcgcgcgcg gtgcgttcgc tgatcctcac ccccacgcgt gagctcgcgg tacagatcga
     4081 ggagtcgttc aaggcctacg gaaaatacgt cgacgtgcgc gcgctcgcgg tctacggcgg
     4141 cgtgcgcatc gaccgtcaat tgaagaagct ccgttccggc gtcgacgtcg tagtggcgac
     4201 accgggccgc ttgctcgacc acctgggccg tcacaccgtc gacttgcgcc acgtcgagat
     4261 cttcgtgctg gacgaagtcg atcgcatgtt cgacatgggc ttcatccaag acgtccgtaa
     4321 gatcat
//
LOCUS       DASPGK010000090         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_139524, whole genome shotgun sequence.
ACCESSION   DASPGK010000090 DASPGK010000000
VERSION     DASPGK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139524"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VEC56_02490"
     CDS             complement(<1..614)
                     /locus_tag="VEC56_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HXV13053.1"
                     /translation="MRSHHRVSPWFLVLCLTVMSFSVPVDAATPVFINELHYDNTGAD
                     ADEGVEIAGPAGTDLTGWRVRLYNGATNLAYGVVVLSGFIPNQCNGFGTIWFPELGVQ
                     NGSPDGLALVDAANNVIQFLSYEGSFVAGDGEAVGLPSTDIGVSEASDSPLGFSLQLG
                     GVGSFYEDFTWAGSAAHSRDACNNGQTFVGTVPVSKTTWGGVKA"
     gene            complement(764..2083)
                     /locus_tag="VEC56_02495"
     CDS             complement(764..2083)
                     /locus_tag="VEC56_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV13054.1"
                     /translation="MARRRSTVDAPGQSHPGVHLNLSVRGMTPSATLAIQERCAALRA
                     ERRDVYRLGLGQSPFPVPRPVVEELRVNAHQKDYLPVRGLPALREAVAEYNIRTQGVP
                     RTAEDVLIGPGSKELMFILQLVYYGEIVIPSPAWVSYAPQARIAGRQIHWVHTRRQNG
                     WRLTPAELEAVCRDDPDRPRLLVLNYPSNPTGGSFTADELQAIADVAREYRVVVLSDE
                     IYGELTFSGQHVSIARFYPEGTIISSGLSKWCGAGGWRLGTFSFPGTMDWLLEAMATV
                     ASETYTSTSAPIQYAAVQAFRGSETLEQYLWLARRILGALGSWSASRLEAGGAWVLPP
                     AGAFYLFPDFTPLSDKLRARGITSSRTLCEKLLDETGVATLPGTEFGRPPNELTVRAS
                     LVDFDGAKAMAGLEVLSRDAPVTEEFLRGHCGRTLRAIELMGDWVGQ"
     gene            complement(2083..3489)
                     /locus_tag="VEC56_02500"
     CDS             complement(2083..3489)
                     /locus_tag="VEC56_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HXV13055.1"
                     /translation="MIRKSRIQPRKYRAFTERDIDRIPQLQAIDADRRLRMKAVARVL
                     PFRVNEYVVDELIDWRRVPDDPMFQLTIPQEGMLDPRDLDRVVSLLRAGASEAKIKAA
                     AREIQMGLNPHPAGQMELNVPSLDGEPVPGLQHKYRETVLVFPSQGQTCHAYCTYCFR
                     WAQFVGIDDLKFAARESESLAAYLKSHKEVQSVLFTGGDPMIMKTSVLRRYIEPLLEN
                     EFKHLVSIRIGTKAPAYWPYRFITDPDADDLLKLFEQMRKAGRHVAIMAHYSHPRELE
                     TPAAQEALRRIVDTGAVVRCQAPIVRHVNDNAKAWSEMWRTQIALGAVPYYMFVERDT
                     GPKNYFEVPLARALDIFTNAYTQLSGLARTVRGPSMSATPGKVLVDGVAEVRGEKVFV
                     LKFLQAREPEWVNRPFFAKFDARATWLDDLVPAFDESQFFFERSMEEIKRGHRHPAWG
                     DKTERRRPLVLFGSVEWE"
     gene            complement(3564..>4004)
                     /locus_tag="VEC56_02505"
     CDS             complement(3564..>4004)
                     /locus_tag="VEC56_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXV13056.1"
                     /translation="ALGPGNRLVSEILSGETTASAQLVLVRVLSTGVSYLSGCAGGVF
                     APFLAIGASAGSWISTWWPGSNAILLALLGMIAVLTGLTRAPLTSFVLILEMTDRHSA
                     IFPMMLTALVALGSSRMAGGTSFYEQTKRLIVSDVEQRRGRDAS"
BASE COUNT          724 a         1314 c         1287 g          679 t
ORIGIN      
        1 atcgccttca caccgcccca cgtggtcttc gacaccggca cggttcccac gaaggtctgg
       61 ccgttgttgc acgcgtcgcg cgagtgcgcg gcgctcccgg cccacgtgaa gtcctcgtag
      121 aatgaaccca cgccgcccaa ctggagcgag aaccccagag gagagtcgct cgcctcgctc
      181 acgccgatgt cggtgctggg caatccgacg gcttcgccgt cgccggcaac gaacgaacct
      241 tcgtagctca agaactgaat gacgttgttg gccgcgtcga ccagcgccaa gccatcgggg
      301 ctcccgttct ggactcccag ttcgggaaac cagatcgttc cgaacccgtt gcactgattg
      361 ggaatgaagc ccgacagcac gacgacgccg taggccagat tggttgcacc gttgtagagc
      421 cgaacccgcc agcccgtgag gtcggttccc gcggggccgg cgatttcaac gccctcgtcg
      481 gcgtctgcgc cggtattgtc gtagtgaagc tcgttgatga agacgggggt cgccgcgtcg
      541 accggaacgg agaatgacat cacggtgagg cacaacacca gaaaccaggg ggatacgcgg
      601 tggtgtgacc gcatggttta gacctcctag cccgcccgag aggaggcgca atcacgaggg
      661 gggcgcttcg cacggaccga gcttgagtga tttgcactgc atcgcgacat ccgagcgcag
      721 agagcgtacc accgcgggcg ttattcgtca acttgatggc atgttattgc cccacccagt
      781 cgcccatcag ctcgatggcg cgcagcgtgc gcccgcagtg cccgcgcagg aactcctccg
      841 tcacgggcgc gtcgcgcgag agtacctcga ggccggccat ggccttggcc ccgtcgaagt
      901 cgaccagcga cgcacgcacc gtcagttcgt tgggcgggcg gccgaactcg gtgccgggca
      961 aggtcgcgac gccggtttcg tcgagcagct tttcgcacag cgtacgactg ctggtgatgc
     1021 cgcgcgcgcg cagcttgtcc gacaacggcg tgaaatccgg aaacaggtag aacgcccctg
     1081 cgggcggcag gacccacgca ccccccgctt ccagccgtga cgcggaccac gagcccagag
     1141 cgcccaggat gcgccgcgca agccagaggt attgctcgag ggtctcactc ccgcggaacg
     1201 cttgcacggc cgcgtattgg atcggcgccg aagtggacgt gtaggtctcg ctcgcgaccg
     1261 tcgccatcgc ctccaagagc cagtccatgg tgccgggaaa cgaaaacgtc cccaaccgcc
     1321 agccgcccgc cccacaccac ttcgacaagc cgctcgagat gatggtcccc tccgggtaga
     1381 agcgcgcaat ggaaacgtgc tgaccggaga acgtgagttc gccgtagatt tcgtcggaaa
     1441 gcacgaccac gcggtactcg cgcgccacgt cggcgatcgc ctgcagttcg tccgcggtga
     1501 acgagccccc ggtgggattg ctgggataat tgagaacgag caggcggggc cggtcggggt
     1561 cgtcgcggca aaccgcttcc agctcggcgg gtgtcaggcg ccacccattc tggcgtcgcg
     1621 tgtgcaccca atgaatctgc cggccggcga tgcgcgcctg gggcgcgtac gaaacccacg
     1681 ccgggctcgg aatgacgatt tctccgtagt agaccagctg gagaatgaac atcaattcct
     1741 tcgaacccgg cccgatcagc acgtcctccg cggtacgcgg cacgccttgt gtgcggatat
     1801 tgtactcggc gaccgcttcg cgcaacgcgg ggagtccgcg cacgggcagg tagtccttct
     1861 ggtgcgcgtt gacgcgcaac tcttcgacca ccggacgcgg caccggaaag ggcgactgcc
     1921 ccagccccag acgatagaca tcgcgacgtt ccgcgcgcag cgccgcacat cgctcctgga
     1981 tcgcgagcgt tgccgatggc gtcatgcctc gcacgctcaa gttcagatgc acgccggggt
     2041 ggctctggcc gggggcgtcc accgtgctcc gtcgtcgtgc catcactccc actccaccga
     2101 gccaaagagc accagcgggc gccgccgttc ggtcttgtca ccccacgcgg ggtgacgatg
     2161 accgcgcttg atctcctcca tgctgcgctc gaagaagaac tgcgactcgt cgaaggccgg
     2221 caccaggtcg tcgagccagg tggcacgcgc gtcgaacttg gcgaagaatg gccggttgac
     2281 ccactccggt tcgcgcgcct gcaggaattt cagcacgaat accttttcgc cccgcacttc
     2341 ggcgacgccg tccaccagca ccttgcccgg tgtcgccgac atggagggtc cgcgcaccgt
     2401 gcgcgccaga ccggaaagct gcgtatacgc gttcgtgaag atgtcgagcg cgcgggcgag
     2461 cggcacctcg aagtaattct tggggccggt gtcgcgctcg acgaacatgt aatacggtac
     2521 cgcacccagc gcgatttgcg tccgccacat ctccgaccac gccttggcgt tgtcgttgac
     2581 gtgccgcacg atgggtgctt ggcagcgcac cacggcaccc gtgtcgacga tgcgtcgcag
     2641 cgcttcctgc gcggcgggcg tctccagctc gcgcgggtgg ctgtagtgcg ccatgatggc
     2701 gacgtggcgc ccggccttgc gcatctgctc gaacagcttg agcaggtcgt cggcgtcggg
     2761 atcggtgatg aaacggtagg gccagtacgc cggcgctttg gtcccgatgc gaatgctcac
     2821 caggtgcttg aactcgttct cgagcagcgg ctcgatgtag cggcgcagca ccgacgtctt
     2881 catgatcatc ggatcgccgc cggtgaacaa cacgctttgc acttccttgt gcgacttcaa
     2941 ataggcggcc agcgattcgg actcgcgcgc ggcgaacttg aggtcgtcga tgccgacgaa
     3001 ttgcgcccag cggaagcagt acgtgcagta ggcgtggcag gtctgcccct ggctgggaaa
     3061 gacgagcacg gtctcgcggt acttgtgctg caggccgggc accggctcgc cgtcgagaga
     3121 gggaacgttg agctccatct ggccggccgg atgcgggttt agtcccatct ggatttcgcg
     3181 cgccgccgcc ttgatcttcg cctcggaggc cccagcccgc agaagactca ccacgcggtc
     3241 caggtcgcgc gggtccagca tgccctcttg gggaatggtg agctggaaca tggggtcgtc
     3301 ggggactcgt ctccagtcga tgagctcgtc gacgacatat tcgttcacgc gaaacgggag
     3361 cacgcgcgcg accgctttca tgcgcaggcg tcgatcggca tcgatggcct gcaactgcgg
     3421 gatgcgatcg atatcgcgct cggtgaacgc gcgatacttc ctgggttgga tccgggattt
     3481 gcgaatcatc cgggctcctt tcggaagatg gatattaacg atgtatcttg acgatatgcg
     3541 atgacattcg attccgcaac cttttacgag gcatctcttc cgcgccgctg ttcgacgtcg
     3601 gacacgatca ggcgcttggt ctgctcgtaa aaggaggtcc cgcccgccat gcgcgaactt
     3661 ccgagcgcga ccagcgcggt cagcatcatg ggaaagatgg cgctgtggcg gtcggtcatc
     3721 tcgagtatca acacgaacga cgtgagcggc gcgcgcgtca gccccgtcaa caccgcgatc
     3781 atccccaaga gcgcgagcag gatcgcgttc gaacccggcc accaggtcga aatccaggac
     3841 cccgcgctgg caccgatggc gagaaacggc gcgaacacac cccccgcgca gccgctcaag
     3901 tacgagacgc cggtggatag tacgcgcacg agcaccaatt gagcgctggc cgtggtctcg
     3961 cccgaaagaa tctccgagac caatcggttg ccggggccca gggc
//
LOCUS       DASPGK010000091         6946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_257881, whole genome shotgun sequence.
ACCESSION   DASPGK010000091 DASPGK010000000
VERSION     DASPGK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257881"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..3173)
                     /locus_tag="VEC56_02510"
     CDS             complement(12..3173)
                     /locus_tag="VEC56_02510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HXV13057.1"
                     /translation="MNRFSSLASYVPLWCKSNFSFLEGASHPDELVEEAARVGIRSLA
                     LTDRDGVYGLVRAFVKARELGVQLIAGSQVTTEDGSPIVLLAMDRRGYANLCNLISDG
                     RLRSPKGESMVRWREVCERAEGLVALWGGDGSLLAGVPDPVFVARDLKDAFGDRLYAM
                     VTRHRTAEDVVAEKRVRERAARFGLATVAAVEVLYHTRARRDLQDVLTCLRHGVTLSS
                     AGRLTRSNAEHALRSPYAFHVLFEDDRASIARTEEVASRCAFTLAEIRYRYPLESLPD
                     GTSAFEHLRRLTFEGAKERYPRERFPAGVPRETAAQLEKELEVVRELEYEGYFLTMYE
                     IVQFCRRNRILCQGRGSAANSAVCYCLGITAVDPVRMELLFERFLSKERAEPPDIDLD
                     IEHDRREEVIQWVYEKYGRTHAAMAANVVRYRARSAVREVGKALDIPDTTLDRIARLL
                     SHYDEITPDGLASAGLDLEAPAHQHLVRLTSEIQDFPRHLSIHPGGFLLGHEPVRDLV
                     PIENATMENRTVIQWDKDDLESIGLFKVDLLGLGMLTVVNRAFDLIDAHHRQKLDMAT
                     VPADDEPTYEMIRRADTVGVFQIESRAQMAMLPRLKPRNYYDLVIEVSIVRPGPITGG
                     MVHPYLRRRNGEEEVVYPHECLEPVLKKTLGIPLFQEQVMKLAVVAADYTPGEADQLR
                     RDMAAWRRSGRLERHHDKLISRMRAKGIEAEFAERVFEQIRGFGDYGFPESHAASFAL
                     IAYVSAWLKRHYLDAFTCAILNAQPMGFYSPATLVDDAKRHGLLVLPVCVCTSDWDCV
                     MEKAPASMRTLGLRNPGDVGVQSFAVRMGLRYVKGLREDFAARLTTARAERAFVSVDD
                     FVQRTGCDERMRTRLAEAGAFEALERGRREALWQARGSLAAPPMELAGSEREVAFRAL
                     SELEKVVWDYETTGHSPRGHPLGPLRDRLAALGLPDARAVQAMPNGKRVRYAGLVIVR
                     QRPGTAKGVVFMTLEDETGFVNLVLWQDVFNRFALVAKTSSFLGVTGKIQRESNVVHV
                     VVESLWEPESLSRKASVRSRDFH"
     gene            3287..3799
                     /locus_tag="VEC56_02515"
     CDS             3287..3799
                     /locus_tag="VEC56_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV13058.1"
                     /translation="MTTQTATPTADAALERDTRDLYEALTDLVRVYQFRDRNCICCHD
                     ISVTQCYALDALVRAGSLSLGSLASTLMLDKSTTSRVVDALDRKGYVKRTVHPDDARV
                     VRLTATIRGRNLHQTIRRDLLNEERAVIESLPAEVRRATVGVVRELAKVAAKRIGRVA
                     PCCGDDACET"
     gene            3868..4308
                     /gene="arsN2"
                     /locus_tag="VEC56_02520"
                     /pseudo
     CDS             3868..4308
                     /gene="arsN2"
                     /locus_tag="VEC56_02520"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
     gene            4450..5253
                     /locus_tag="VEC56_02525"
     CDS             4450..5253
                     /locus_tag="VEC56_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HXV13059.1"
                     /translation="MRAVVREKYAQAARKAADGSSACCGSSCGCSNPITDDLYDASQT
                     AGLPETAMLASLGCGNPTLLAELREGETVLDLGSGGGIDVLLSARRVGPKGFAYGIDM
                     TDEMLALARENQRKAGAINVEFRKGEIEALPLPNATVDVIISNCVINLSADKRRVLAE
                     AFRVLKPGGRFAVSDIVVRREIPEAIRRHAELWAGCVAGALEEWTYRDLLREAGFVDI
                     EVVPTRIYGGDDAREIASGAGLDSATLAVLDGAFMSAFVRARKPSEGGR"
     gene            complement(5448..>6946)
                     /locus_tag="VEC56_02530"
     CDS             complement(5448..>6946)
                     /locus_tag="VEC56_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HXV13060.1"
                     /translation="RKHPSWSGHPAAVVDRDVAQGVILWSNERARACRVLPGLRYAAA
                     LSLSPDLHAGEVPAPEIQHTIDSLTDALRFYTPDVEPSDAEPGVFWLGASGLSLLYPS
                     LRKWAAWVEREVARVGFSASVVVGFSRFGTYALARSDPKPRAEVLDTEADERARAALV
                     PLDRLGIAPDVRDTLGKLGVATLGDFLALPANGVRTRFGADAHRMHQIASGELAAPLQ
                     PRPAREPVASTVHLDYPETDLERLMAVIDRSIQSLARTLDERGEVLRAIALQLEFDDG
                     TKTAERLEPASPTLDLSQIVELVRLRLTGVLAARAEPEAGPARSRAIKDSVRARGVTG
                     VRVEMDGVSASHTQADLFDRKPRRDPAVAARAMARVRAELGASAVVRAVLREGHLPEA
                     RFDWEPVASLDAAQPRAVRQPPLVRRIYARPIPFSPARHRDAEAELLKHIDEGTVRET
                     FGPYIVAGGWWAREVQREYYFVRTSNGRALWTYYDRRRMGWFIQGEVE"
BASE COUNT         1142 a         2303 c         2407 g         1094 t
ORIGIN      
        1 gaagaactgc gttagtggaa atcccggctc cgcacgctcg cttttcgcga aagcgactcc
       61 ggctcccaca gcgattccac caccacgtgc acgacgttcg attcgcgctg gatcttgccc
      121 gtcaccccca agaacgacga cgtcttcgcg accagcgcga agcgattgaa cacgtcttgc
      181 cataagacga ggttcacgaa cccggtctcg tcttcgagcg tcatgaacac cacgcccttc
      241 gcggtgcccg ggcgttggcg cacgatgacg agccccgcgt agcgcacgcg cttgccgttg
      301 ggcatcgctt gcaccgcgcg cgcgtcagga aggccgagcg cagcgaggcg atcgcgcagc
      361 ggccccaacg gatgcccacg cgggctatgg cccgtggtct cgtaatccca taccaccttc
      421 tccagctcgg agagcgcgcg gaacgcgacc tcgcgctcgg agcccgcgag ctccatcggc
      481 ggcgcggcga gtgacccgcg cgcctgccac agcgcctcgc gccgtccgcg ctcgagcgct
      541 tcgaaggcgc ccgcttccgc caagcgcgtg cgcatgcgct cgtcgcagcc ggtgcgctgc
      601 acgaagtcgt ccacggaaac gaacgcgcgc tctgcgcgcg ccgtcgtcag gcgcgccgcg
      661 aaatcttccc gtaacccctt tacgtagcga agtcccattc ggacggcaaa tgattgcaca
      721 ccgacgtctc ccggattgcg tagccccagt gtgcgcatcg aagcgggggc tttctccatg
      781 acgcagtccc agtcgctggt gcacacgcat accggcagca cgagcaaacc gtggcgcttg
      841 gcgtcgtcga cgagggtggc gggggagtag aagcccatgg gctgcgcgtt caagatcgcg
      901 cacgtgaacg cgtccaaata gtgccgcttg agccacgccg acacgtacgc gatcaaggcg
      961 aagctggccg cgtggctctc ggggaatccg tagtcgccga agccgcgaat ctgctcgaac
     1021 acgcgctcgg cgaactcggc ctcgatgccc ttggcacgca tgcgcgagat gagtttgtcg
     1081 tggtggcgct cgagtctccc gctgcgccgc caggcggcca tgtcgcggcg cagctggtcg
     1141 gcttcgccgg gggtgtagtc ggcggccacc accgcgagct tcatcacctg ctcctggaag
     1201 agcgggatgc cgagggtttt tttgaggacc ggttccaggc actcgtgggg gtagacgacc
     1261 tcctcttcgc catttcgccg gcgcaagtac gggtgcacca tcccgcccgt gatggggccg
     1321 ggcctcacga tgctaacttc aatgacgaga tcgtaatagt tgcgcggctt caagcggggc
     1381 agcatggcca tctgggcccg gctctcgatc tggaacacgc ccaccgtgtc ggcgcggcgg
     1441 atcatctcat aggtgggctc gtcgtcggcg gggacggtgg ccatgtcgag cttctggcgg
     1501 tggtgcgcgt cgatcaagtc gaaggcgcgg ttgaccaccg tcagcatgcc gaggcccaag
     1561 aggtcgacct tgaacaggcc gatcgattcc aggtcgtcct tgtcccactg gatcaccgtg
     1621 cggttctcca tggtggcgtt ctcgatgggg accaagtcgc gcacgggttc gtggccgagc
     1681 aggaatcctc cggggtggat cgacaagtgg cgcgggaaat cctggatctc ggaggtcagt
     1741 cgcacgaggt gctggtgtgc gggggcttcg aggtccaagc ccgcgctcgc gagaccgtcg
     1801 ggcgtgatct cgtcgtagtg cgagagcaaa cgcgcgatgc ggtcgagcgt ggtatccgga
     1861 atatcgagcg ccttgcccac ctcgcgcacc gccgaacgcg cgcggtagcg caccacgttg
     1921 gccgccatgg ccgcgtgcgt acggccgtat ttttcgtaca cccactggat cacttcctcg
     1981 cggcggtcgt gctcgatgtc gaggtcgatg tcgggcggct ccgcgcgctc tttggaaaga
     2041 aagcgttcga agagcagctc catgcgtacc gggtcgaccg cggtgatgcc gaggcagtag
     2101 cacaccgccg agttcgccgc cgagccgcgg ccctggcaca gaatgcggtt gcgacggcag
     2161 aactgcacga tttcatacat ggtcaagaag tagccctcgt actcgagctc gcgtacgact
     2221 tcgagctcct tctccagttg cgccgcggtc tcgcgtggaa cgccggcggg gaagcgctcg
     2281 cgcgggtagc gctccttggc gccctcgaag gtcaagcggc gcaggtgctc gaaggcgctg
     2341 gtgccgtcgg ggagcgactc cagcgggtag cggtagcgaa tctccgcgag tgtaaaggcg
     2401 cagcgggacg caacctcttc ggtgcgcgcg atcgacgcgc ggtcgtcttc gaacaacaca
     2461 tggaatgcat agggtgagcg cagcgcgtgc tcggcgttgg accgcgtgag acggcccgcg
     2521 ctcgagaggg tgacgccgtg gcgcaggcac gtgaggacgt cctgcagatc gcggcgcgcg
     2581 cgtgtgtgat agagcacttc gaccgcggcc acggtggcga gcccgaagcg cgcggcgcgc
     2641 tcgcgcacgc gcttctcggc cacgacgtct tcggcggtgc ggtgccgcgt caccatcgcg
     2701 tacagccggt cgccgaaggc atccttcaag tcgcgcgcga cgaacacggg gtcgggaacc
     2761 cccgcgagca aactcccatc gccgccccag agcgcgacga gtccctccgc gcgctcgcac
     2821 acctcgcgcc atcgcaccat actctcgcct ttgggagagc gcaggcgccc gtcggagatc
     2881 agattgcaca ggttcgcgta gccgcggcgg tccatcgcga gcagcacgat gggcgagccg
     2941 tcctcggtgg tgacctgcga ccccgcgatc aattggaccc cgagctcgcg cgctttgacg
     3001 aaggcgcgca ccagcccgta gacgccgtcg cggtcggtca gggcgagcga gcggatgccg
     3061 acgcgggcgg cttcttcgac caattcgtcg gggtggctgg ctccctccaa gaaagagaag
     3121 ttggatttgc accacagggg gacgtaggac gcgagggagg agaagcggtt catggcgttc
     3181 cccatgctag cagacaattg tcttgtgtct cggcaaaaaa agtcccttga ccggccgcgg
     3241 cgcccaggct atagttggca ctgacaacta acgaggagcc actcctatga cgacccagac
     3301 cgccaccccg accgccgacg ccgccctcga gcgcgacacg cgcgacctgt acgaggcgct
     3361 caccgacctc gtgcgcgtgt atcaatttcg cgaccggaat tgtatttgct gccacgacat
     3421 atcggtcacg caatgctacg cgctggatgc gctggttcgc gcaggctccc tatccctcgg
     3481 ctcgcttgcg tcgacgttga tgctcgacaa gagcacgaca agccgcgtcg tggatgcgct
     3541 cgaccgcaag ggttacgtca agcggaccgt ccaccccgac gatgcccgcg tggtgcggct
     3601 gacggccacg attcggggac gaaatctcca ccagaccatc cgtcgcgacc tgctcaacga
     3661 agaacgcgcg gtgatcgaga gtcttccggc cgaggtgcgt cgcgccaccg tgggtgtggt
     3721 gcgcgagctg gccaaggtgg cggccaagcg catcgggcgg gtggcaccgt gctgcggcga
     3781 cgacgcgtgc gagacctaat tgttctttgg acaagatgtt gttactagca accatatgag
     3841 gcgaacgata tgctggccac cgaccgtatg aacccgagag acgaatcgac ccgcgcgggg
     3901 tggaccctgc gcccggcgcg cgccagcgac ctcgccgcgg tgtgcgcgct cctggcatcg
     3961 tgcaagctcc ccgagcaggg cgtcgaggac caattcggcg acggctacgt ggtcgccgaa
     4021 cacgggagcg aaatcgtcgg cgtcgccggc atcgagcgcc acggcccgta cggcttgttg
     4081 cgttcggtgg cggttacgcc cccgtggcgc ctggccggcg tgggcgcggc actcgtgctc
     4141 gatcgtgtgc agtggtcgga ggaagcgggc gtgaagtcgc tctacctgct caccaccgac
     4201 gccgcgcgct atttccaccg ttacggattc cgcgccctcg ctcgcgacaa cgcccccgcg
     4261 gagattcgcg cctcgcgcga attctcggag acgtgtccca cgtcggcgcg cgtgatgcgc
     4321 ttcgacgccg acgccgccat gcgcggcccc gaggacgaat gctgcggcgg caactgcccg
     4381 tgcaaggacc acgcggggtc gaagacggcc gtgtggtcct ccgcgcagac gaccgacgac
     4441 ggcgagggga tccgcgcggt ggtacgcgag aaatacgcgc aggcggcgcg caaggcggcg
     4501 gacggctcgt cggcgtgctg tgggagcagc tgcggttgca gtaatcccat cacggacgat
     4561 ctctacgacg cgtcgcagac cgcaggactg ccggagaccg cgatgctggc ctcgctcggg
     4621 tgcggcaatc ccacgctcct cgcggaattg cgcgaaggcg agaccgtgct cgacttgggc
     4681 tcgggcgggg gaatcgacgt attgctgtcg gcgcggcggg tggggccgaa ggggttcgcc
     4741 tacggcatcg acatgaccga cgagatgctg gccctggcgc gcgagaacca gcgcaaggcg
     4801 ggtgcgatca acgtggagtt tcgcaagggt gaaatcgaag cgctcccgct cccgaatgca
     4861 accgtcgacg tcatcatctc caactgcgtg atcaatctct cggccgacaa gcgtcgcgtg
     4921 ctggcggagg cgtttcgcgt cttgaagccg ggcgggcgct tcgcggtgag cgacatcgtg
     4981 gtgcggcgcg agatcccgga ggcgattcgt cgtcacgccg agctgtgggc cggctgcgtg
     5041 gcgggtgcgc tcgaggagtg gacctaccgc gacctcctgc gcgaggccgg cttcgtcgac
     5101 atcgaagtcg ttccgacgcg gatctacggc ggtgacgacg cgcgcgagat cgcgtcgggc
     5161 gccggtctcg attccgccac cctggcggtg ctcgacggcg ccttcatgag cgcgttcgtg
     5221 cgggccagga agccctccga aggagggcgg taggcgttcg gcccggcgct cgcgccgcgc
     5281 gcgtgcacac ctccatgcga gcagtgagaa gcgaatggga atcacacgac gaaaggagga
     5341 cgccatgaaa aacacgtgtt cgtgcggatg cggtggtggt tgttgcggcg gcggggacta
     5401 gtcccacgtc gagcgggcgc gcggcgaaaa ggagtcgcgc gcccgcttca ctccacctct
     5461 ccctgaatga accatcccat ccgacgccgg tcgtagtacg tccataacgc gcgcccgttc
     5521 gaggtgcgca cgaagtagta ttcacgctgc acctcgcgcg cccaccatcc gcccgccacg
     5581 atgtagggtc cgaacgtctc gcgcaccgtg ccttcgtcga tgtgcttcaa tagctccgct
     5641 tccgcgtcgc gatggcgcgc gggcgagaag ggtatgggac gcgcgtagat gcgccgcacc
     5701 agcggcggct ggcgcaccgc gcgcggctgt gcggcgtcga gcgatgctac cggctcccag
     5761 tcgaagcgcg cttcgggcaa gtggccctcg cgcagcaccg cccgaaccac cgcgctcgct
     5821 cccagctcgg cgcgcacgcg ggccatcgcg cgtgcggcga cggccgggtc gcgacggggc
     5881 ttgcgatcga agagatccgc ctgggtgtgc gacgccgaca cgccgtccat ctcgacgcgc
     5941 acgccggtaa cgccgcgggc tcgcacgctg tcctttatag cgcgcgagcg cgctgggccc
     6001 gcttccggct cggcgcgggc cgcgagcaca ccggtcaagc gcagccgcac cagctcgacg
     6061 atctgcgata aatcgagagt gggcgaggcg ggctccagtc gctccgcggt tttggtaccg
     6121 tcgtcgaact cgagctggag tgcgatcgca cgcagcacct cgccgcgctc gtcgagggtt
     6181 cgggccagcg actgaatgct gcgatcgatc accgccatca atcgttccag gtcggtctcg
     6241 gggtagtcga ggtgcaccgt cgatgcgacc ggctcgcgcg cggggcgcgg ctgcagcggg
     6301 gcggccagct cgccgctcgc gatctggtgc atgcgatgcg cgtcggcgcc gaagcgggtg
     6361 cgaacgccgt tggcggggag ggcgagaaag tcacccagtg tggccacgcc aagtttgccg
     6421 agcgtgtcgc gcacgtcggg ggcgatcccg agccggtcga gcggcacgag ggccgcgcgc
     6481 gctcgttcgt cggcttcggt gtcgaggacc tcggcgcgcg gcttcgggtc cgagcgcgcg
     6541 agcgcgtacg tgccgaagcg cgaaaacccc accaccaccg aggcggaaaa gcccacacgc
     6601 gcgacttcgc gctccaccca cgccgcccac ttgcgaagcg acgggtagag cagcgatagc
     6661 cccgatgccc ccagccagaa cacccccggc tccgcgtcgg agggctcgac gtcgggcgtg
     6721 tagaagcgaa gcgcgtcggt cagcgagtcg atggtgtgct ggatctcggg cgcgggaact
     6781 tcgcccgcat gcaggtcggg ggaaagcgaa agggcggcag cgtagcggag accgggaagg
     6841 acgcggcacg ccctcgcgcg ttcgttggac cacagaatga cgccctgcgc aacgtcgcgg
     6901 tcgacgaccg cggcggggtg cccgctccag ctcgggtgct tacgca
//
LOCUS       DASPGK010000092        21430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_842969, whole genome shotgun sequence.
ACCESSION   DASPGK010000092 DASPGK010000000
VERSION     DASPGK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 21430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21430
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842969"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VEC56_02535"
     CDS             complement(<1..689)
                     /locus_tag="VEC56_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HXV13061.1"
                     /translation="MVFWIVLVLVLLVAFQMFEMGKEPQYRISYSEFLGQVDRGNVKK
                     ITFKGLEVRGQLATPTLVPVPTTTERGRTREVKVEQFLLVLPAEDKDLPQRILEKNPT
                     AVIEGQIPGGSTWVKVLGSAIPLLLIFGIWLLFMRQMQVGGNRAFSFGKSRARMFGGD
                     RPRVTFNDVAGCDEAKEELQEIIEFLKEPKKFQKLGGRIPKGALLLGPPGTGKTLLAR
                     AVAGEAGVPFF"
     gene            complement(799..1353)
                     /gene="hpt"
                     /locus_tag="VEC56_02540"
     CDS             complement(799..1353)
                     /gene="hpt"
                     /locus_tag="VEC56_02540"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012808203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXV13062.1"
                     /translation="MSRNHPDHPSPGQVLVTAAEIDDIVARLGREISRDYSSRHPVLI
                     SLLKGGVVFLADLIRHLTIPHHIEFMRVSSYESTRSTGAVRIISDLDTSIEGRDVLLV
                     EDVIDTGTTLAYIVDMLRLRNPGSLEVCALLRKRVASRPAGIPIRYLGHDIPDTFVVG
                     YGLDHDENYRNLPFIAALDPSLGR"
     gene            complement(1350..2714)
                     /gene="tilS"
                     /locus_tag="VEC56_02545"
     CDS             complement(1350..2714)
                     /gene="tilS"
                     /locus_tag="VEC56_02545"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXV13063.1"
                     /translation="MILPLEHPVERALVASALLPRDGVVLVAVSGGADSMALVDALRA
                     VVQPVVAHFDHRLREDSAHDAEHVAAYARAHGLEFVLGSEDVARRARAERASIEEAAR
                     NARYQFLTATAKHLGASCIATAHTQTDQVETVLMRILRGAGRVGLAGIPARRGSFVRP
                     LLGITRADTRDYCASRGVVFVDDPTNEDVQFARNRVRHQILPELRAMFPSIDDALLRI
                     AEGARGEQRAFEARTDVRRVVREEESGVWVLGLDAFEALDDDDATALLRDALAHAGLA
                     HDVGRVHYQRLLELVRSTHAGSSVDLPRVRARREHDAIVLRPREAASERPAALLTIPG
                     RTRAGDWLIETSFIDAAAARRELAGASRDATTAYFDADAVSTRLVARAPREGDRMRPF
                     GLDGRKKLSDLFVDRKVPRRARERALVVEGETIHWVPGVATSDDGRVLPETARVVRMK
                     ATRA"
     gene            complement(2752..2828)
                     /locus_tag="VEC56_02550"
     tRNA            complement(2752..2828)
                     /locus_tag="VEC56_02550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2792..2794),aa:Met,seq:cat)
     gene            complement(2964..4910)
                     /locus_tag="VEC56_02555"
     CDS             complement(2964..4910)
                     /locus_tag="VEC56_02555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV13064.1"
                     /translation="MPPSVTPRRKRDTPRDQNAASAVRRPSRRWLLVAALFLFSVVLV
                     RTAWVTDDTYITYRVVDNCLHGYGLTWNTDERVQAYTHPLWLFLNLVFQSVTREAYLT
                     GIVLSIAVSLSALVLLAFRVARTTNAALLGLLILLLSRAFVDYATSGLENPLTHLLLA
                     LFILTWFRTPRDVRRFFHLCAVAGLGILTRADFALLVGPALVVAAKGLPKRAALKTAV
                     VAFLPVIAWETFSLVYYGSLVPNTAYAKLNTGIQRSELVTQGVHYLIESLRRDPLTLV
                     VIAFGIAVPFLVRRERLVPLVFGIAAYLAYTVWIGGDFMSGRFLTPPLFAAVAVLSQF
                     RFQSRRVLWPAAAAAVLTGLAPGRPTVTSGRSHGLEKRAMVRGIADEREFYFQHTGLF
                     RADGKRGVDHPKATEGRGARGVGRSVQLGGMVGFLGYYSGPDAHILDGYALADPLLAR
                     LPERGIKWMIGHFAREIPAGYMLTLVRGENLIEDERIAEFYDAVRVVTRGPIFDGRRA
                     RVVWKMNTGGYDHLLQPFFQARRLQEDAVRELAAGRTDACIAAAERAVALDPRRAAAW
                     SLISRASLQAGDLARASDCALKAAAIIPTIHATDALAVGDACEQQGDVAAAIAVYERL
                     LEIDPKQAVAAERVSRLRGSPADR"
     gene            5013..5513
                     /locus_tag="VEC56_02560"
     CDS             5013..5513
                     /locus_tag="VEC56_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13065.1"
                     /translation="MRFHAASFALAVVVAPLFAMAESSQPMPPEASQFDFWVGEWDAT
                     WGDQQRGINRVTKRWDTVVVEEFDGKPGLQLEGHSVSVFDANAKQWKQTWVDNQGGYL
                     DFTGGFADGRMTLSRSFQKDGQTIHQRMVWYDITPDRFEWNWERSLDAGKTWEVQWQI
                     HYARRK"
     gene            5607..6617
                     /gene="ilvC"
                     /locus_tag="VEC56_02565"
     CDS             5607..6617
                     /gene="ilvC"
                     /locus_tag="VEC56_02565"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018921644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXV13066.1"
                     /translation="MNVYAHHDLKQNLLDGATVAVLGFGNQGHAHALNLRDSGARVVV
                     GARRNGAGWKAAKAADFETLPIGDATARADVVVMLLPDEEQASVFASEVAPSLREGAV
                     LVFAHGFMVAFGGLNTGSSHDVVLVAPKGQGHFLRREYQAGRSVPCMVGVETDISGRA
                     LQWALSYASHLGCLRAGAIATSFREEAITDMFGEQAVLCGGVPALVKAAFETLVAKGY
                     SPEVAYIECLQELKIIADLMYEGGLADMRARISKTAAWGSFESGPRLVTDATRRALES
                     ILDDIESGRFASQWLEEARAGQRTLAARMHEEETHPIEKAGRAVRALMRRSDGKSNKE
                     ST"
     gene            6614..7528
                     /locus_tag="VEC56_02570"
     CDS             6614..7528
                     /locus_tag="VEC56_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13067.1"
                     /translation="MKGIKMGRIGPRLAVVATAALALASCSEKEAAREKPAVKRYTVP
                     ASLDRELNNAELDSLSRMKRTHNITRDEYWDGYGGVLANDVLEVWYPDGKLNAMQGMA
                     MMKLASEARARTQRVFGVAPAERAVIICAGDIDVYTSSTGREWWHYSRIVGDTISIQP
                     PINLHTRGILTVVGPREYYEWAIGKISKGHAPRWIEEGLASYLAGEAAVLEELRVDTG
                     DKPIAMSTKDMESALEKENDRQETRRAYYNAYRTVEQIVKAHGEEAMGRFVVALGGDD
                     NLDAASQAAFGAKFGEVAERAHAWARAE"
     gene            complement(7533..8858)
                     /locus_tag="VEC56_02575"
     CDS             complement(7533..8858)
                     /locus_tag="VEC56_02575"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HXV13068.1"
                     /translation="MSPRLTSRIAESFWRSNPRPDARVVDEMRLADAPDARPVARHGR
                     EGIAFIEKLARALHRYGSAAPTVEDALALVAERLGIHGEFFATPTAIFASLDTGDIDQ
                     TIMIRVEPGSVNLDKLSRLDRLTQDVADGRVSPAEASRIIDEIRATPPRFRGKTTAIA
                     FAVASAASACFFGGGWREMVACSAIGLVSGLLALLARRNPRFRLFEPLAAFIGGFGAY
                     AAAALMPPTSLYVAMAGGLIVLLPGLTLTTAISELATRHLQSGTSRLMSALIILVSIG
                     FGVALGLEAGERLFGSVPVVNPEALPPWTLAIAVVMASLAFMVLNQAHYGDGGWILLV
                     CVLAFVGSRLGAQWIGVESGAFLGAVLVGVTSGIISHARNRPAIVTATPGTIMLVPGS
                     IGFRSITEMIANDPVSGLHTAFTMILTGVALATGLLVARLIVPRRSLFK"
     gene            8947..9020
                     /locus_tag="VEC56_02580"
     tRNA            8947..9020
                     /locus_tag="VEC56_02580"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8979..8981,aa:Gln,seq:ctg)
     gene            complement(9026..9712)
                     /locus_tag="VEC56_02585"
     CDS             complement(9026..9712)
                     /locus_tag="VEC56_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXV13069.1"
                     /translation="MRRVVYSLALAALAAAMASAQTIPDEKKTIGTQMPDVALVSDAD
                     STFHVSALWGKPVIVSPIFTSCPHTCSMITESLRDALREIGEPGVGYEVLTISFDPAD
                     DSAAMRAYRERLELPAGWRLATASAADLATFLGAIDFTVTALAEGGFAHPNLVVVLSP
                     DLKVASYVHGVMYDADDVRAALEDASERASLVRRFRPLLVVIVGLGLGAVGWALYVTR
                     GAAHRRQREA"
     gene            complement(9718..10158)
                     /locus_tag="VEC56_02590"
     CDS             complement(9718..10158)
                     /locus_tag="VEC56_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXV13070.1"
                     /translation="MLHSSACEYAIRALTHMAGSPVGTRMLARDIAAHERVPGPFLGK
                     IFQTLVRADILNSSKGPGGGFSIAREPSTITLFDIYQAIDGTTYLDGCAVGLARCSDE
                     TPCPLHERWKPLRERIRTYLQTTTLADMAEATRKKRELLARDSG"
     gene            10307..10381
                     /locus_tag="VEC56_02595"
     tRNA            10307..10381
                     /locus_tag="VEC56_02595"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10340..10342,aa:Glu,seq:ctc)
     gene            10453..11289
                     /locus_tag="VEC56_02600"
     CDS             10453..11289
                     /locus_tag="VEC56_02600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV13071.1"
                     /translation="MATRTTGRRARTRTPSGPKSRDRHWLYQESVQSPEVHFGFFERA
                     YRERTGRRPRSLKEDFCGTALLAAQWVEGHRDRTAIGVDLDAPTLRWAQKHNIAPLSD
                     DARARITLRRDNVMHVVKPKVDIVVALNFSYNIFHTREALRAYFEAARRSLTPGGVFI
                     IDMFGGWDSQRPMTERTRHRGFTYLWELHHYDPVANFGQFYIHFKFNDGGGIRRAFSY
                     AWRTWSIPEVREVLLEAGFEDLDLYWEGFDTRTGLGNSVFRKVTRAKNSPGWIAFFVA
                     SA"
     gene            11399..12823
                     /locus_tag="VEC56_02605"
     CDS             11399..12823
                     /locus_tag="VEC56_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV13072.1"
                     /translation="MFFRQVYDPKLSQYAYVIGCQATGEAIVVDPLRDVESYLEIAKS
                     EGLRLVAVAETHIHADFLSGARELCERAGVTAYVSGEGGADWQSEWLKGSAYQHRVLH
                     DGDHFMVGNIRFDVIHTPGHTPEHVCYTVTDTPARAALAVGILSGDFVFVGDVGRPDL
                     LETAAGQAGAMEPSARALFRSIEKFRALPPALQLWPAHGAGSACGKALGAVPVSTVGY
                     ELAANASIGAATDPESFLAYILEGQPEPPLYFARMKRDNRGGPAVLGKMPEPKPLAAS
                     KLVELERATGVALLDLRSWDAYKRAHVPGSLFTPLNRAFNTVAGSYVPEGMAIYLIVD
                     EKRVREATVDLVRVGLDRVIGYATPAVFDEYVRKGKPASIAEIDATKVEAQLDAGAYL
                     LDVRRAAERAERGQIAGSHNIAYERLLARVGEIPKDRPVVVHCQGGTRSAYAAGLLDR
                     MGYSVTNVAGGYDAWKSKAEVAKR"
     gene            12835..13833
                     /locus_tag="VEC56_02610"
     CDS             12835..13833
                     /locus_tag="VEC56_02610"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXV13073.1"
                     /translation="MQFVVYGAGAVGSVLGGMLSLHRHDVLLVGRPALVDAVREGGLR
                     LKSATGEYVSHPRVAAALSAGDVGADTHVLFTVKSYDVPSAVAALAKVAPSTSPVVCF
                     QNGIDSEDVVAKHFARVYGAVVRMTCSMVQPGHASFRALGRLVLGAHPKGTDAVVAEL
                     VAAFRAAGFDAAASKSIVDDKWLKLAVNAQSVVHALVDARDQDENEFFDLKVAILEET
                     RRVYKAAKVKARSGDGKDPSIDEMIAEIKRPRARRNEHGVKVHNSVWQDFYLKRPRIE
                     ADAIHGPVIALGKEHGVATPYHDASLALARRCHVSKSAPESIRLSEVLSEIAKRRS"
     gene            13830..15506
                     /locus_tag="VEC56_02615"
     CDS             13830..15506
                     /locus_tag="VEC56_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13074.1"
                     /translation="MRTIAYGAFLLLLLAASVVLLRSIEPVHEGAGPFPRVTGSLEPV
                     VSAIDLDRLEGAGATELYDLAAEFMQVWRPREATMLLERAVAADSTFYPAWLKLVECY
                     AHPLVGDESALEKAVVRAGTTAPAPADTALVAGLRQLYVDRDYAGAVGALSAVVRAEA
                     ATIDARYHLALAYYQMGRLRDASRYLEPLLSEDPTVAPVVELYIRRAVAARELERAAD
                     AARELARMYAEEPFPYVLSAQVELARGNREAAVEFCDNALDLDPACAPAIMTRACLYA
                     EAGDFQSARVSFEKLLLFDAAALQSIGHEGVAFVDFLAGDFDHGVASMDEATRHAMFA
                     GATRRGLSLAYGHVEYLCQLGQPDAAQGVIERWLTGFGEIPVQMASARVALARGDTRR
                     AAETVARLSAEKEWILWARKLSLDATDLAARVEIGEERQREAAVRLQEDAKSATAVAA
                     GAFERRTFLAGYAAFESGDAESAAAAFSDVHRRLYGVEFPYHGDPVVYVQALFFLAET
                     DLARGNQVAARASYEAFLGYWGDASWKLEAVDRARRKLEALGEATASPQG"
     gene            complement(15547..16809)
                     /locus_tag="VEC56_02620"
     CDS             complement(15547..16809)
                     /locus_tag="VEC56_02620"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXV13075.1"
                     /translation="MSDRMNLFEMSSSQFRKAADLINLNPMVRTILSEPQNEIIVNFP
                     VTMDDGTHRLFKGYRIQHNNTNGPYKGGIRYHESVNLSEVKALASWMTWKSALMGIPF
                     GGAKGGVSFNPRNFSLAELQRITRRFTYALGNNIGPEYDIPAPDVGTGAREMVWMMDT
                     YMQMFNASERNQTRRVVTGKSLINGGSLGRDKATGQGVVFCFDVWAQHVGFEIKGSTF
                     FVQGFGNVGSHAARIMQARGSRLMSVNDHTGSIGNADGIDAEDLAAYVAQKGGVKGYP
                     RAKEIALTEFYHTKADIFIPAALECTVNVDNDSYVQAKFVLEGANGPTTPEAEENLAS
                     RGITVIPDILANSGGVTVSYFEWVQNKNSEIWELEKVDEELNRVMTRATLGVIDEMNA
                     RKCSPRTAAMALAVGRLEKIYMERGIFP"
     gene            18225..18773
                     /locus_tag="VEC56_02625"
     CDS             18225..18773
                     /locus_tag="VEC56_02625"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HXV13076.1"
                     /translation="MDLVPSAMFLTKGMGRHREQLTSFELALRDAGIAHVNLVTVSSI
                     FPPQCRLVPKQRGAAMLHPGQVVFSVIARMSTDEAFRMTACSIGVAIPGDRDKYGYLS
                     EHHAYGQNEKAAGDYAEDLAATMLATTLGIELDTDKAWNQRKEQWKIGRQIVRTTNIT
                     QTAIGKEGLWTTVIAAGVFCKY"
     gene            18784..19806
                     /locus_tag="VEC56_02630"
     CDS             18784..19806
                     /locus_tag="VEC56_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HXV13077.1"
                     /translation="MTLGFLEDKADPGDIVRDENRYVLALLPYEQTTTYGQGVGRAPE
                     AIVQASSHVELFDEVLEVDASRHGILTVRPSITDLASVTRAARKLREEHPHSLIGFVG
                     GEHSLTPALVEAFAPPATGSKPNLGIVWIDAHADLRQEFHGREDNHACAGHNSMRFGP
                     IVQVGIRTLAEEEWSLLKKTDRVRAHRHWNKKARADLLALPRDVYLTVDFDGFSPEVI
                     RAVGTPEPGGLYWEEVMDILDTVFRHKNVVAFDAVELAPNDADIASSFTAARLVYKVM
                     NYHAAYTLGDRRKKAHRATSSSARKKHRRLPRKPKRRARTARARRSRRRTKARSTRMR
                     TTSTAR"
     gene            complement(19787..20122)
                     /locus_tag="VEC56_02635"
     CDS             complement(19787..20122)
                     /locus_tag="VEC56_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13078.1"
                     /translation="MEQKDWEQRLDRVAERVAGSVKEGVRVLEDAYEKGKEGVMNEAR
                     GTGSGASSEAGAGAPGTGATKKGSPRLGLALVALGIIWLLHSLGILDQPVFPILLIIL
                     GVYFIARSK"
     gene            complement(20161..>21430)
                     /locus_tag="VEC56_02640"
     CDS             complement(20161..>21430)
                     /locus_tag="VEC56_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV13079.1"
                     /translation="ESAASIHMKPFRRMSEFSPEEHRILLVTCPGHFFTHFFTLAFPA
                     ITIPLTAAFAMPLEAVVKLSFWMYLLYGLLALPVGLAADRWRAKPMLVAGIAIMGAGL
                     VVAGAYPSPGVMTASLAFVGVGASIYHPAGLALISRTVRQRGVAMGVNGVFGNLGIAL
                     SPLATGALSWAFGWQAALIVLGAIGLITAAWLQTIHVDETIRPAAKPVVHNGVPRREM
                     ARYFGILCVALVLGGIAYRGNMLLLPAYMELKTTFLAKLFEHLPRVDGHSTATFAATV
                     LASIVLCGGLFGQIVGGRLADRMELRRAYLLFHAASFPFVLAMAFSGDVVLVACAVAY
                     EFFSLGMQPIENSLIAAMTPDRWRSTSYAVKFMLTFGVGAWAVHLVAPIKQAYSIEMV
                     YVFLAGVVFLLVMNIVVLMVASRKIPAVRN"
BASE COUNT         3720 a         7061 c         7301 g         3348 t
ORIGIN      
        1 ctgaagaacg ggaccccggc ttcgcccgcg accgcgcgcg cgaggagcgt cttgccggtg
       61 cccggaggac cgagcaggag ggcgcccttc gggatgcggc cgccgagctt ctggaacttc
      121 ttgggctcct tcaagaactc gatgatctct tgcagctctt ccttggcttc gtcgcagccg
      181 gccacgtcgt tgaaggtcac gcgcggacgg tcgccgccga acatgcgcgc gcgcgacttg
      241 ccgaagctga acgcgcggtt gccgcccact tgcatctggc gcatgaacag gagccagatg
      301 ccgaagatca acaggagcgg aatcgcggag ccgaggacct tgacccacgt gctgccgccc
      361 ggaatctgtc cctcgatgac ggcggtggga ttcttctcga gaatgcgctg cggcagatcc
      421 ttgtcctccg cggggagcac cagcaggaat tgctcgacct tgacctcgcg cgtgcgaccg
      481 cgctcggtgg tggtggggac ggggaccaac gtcggtgtcg cgagctggcc gcgcacctcg
      541 agccccttga acgtgatctt cttgacgttg cctcggtcta cttggcccag gaactccgag
      601 tacgagatgc ggtactgcgg ctccttgccc atctcgaaca tctggaacgc gaccaagagg
      661 acgagcacga ggacgatcca gaacaccatc gcgcgcagcg ggcgcgtggg ccggcccagc
      721 ggaggccgcg tcggaagagt gggacgaccc gagggtcgct tatcgggatc tcgataatca
      781 ggcatggcgt gtcgcgcgct atcgacccaa gctggggtcg agtgccgcta tgaagggtaa
      841 gttacgataa ttttcgtcgt ggtccagccc gtatcctacc acgaacgtat ccgggatatc
      901 gtggccgagg tatcgaatgg gtatgccggc ggggcgggag gcgacgcgct tacgcagaag
      961 ggcgcacact tcgaggctgc cgggattccg taacctcaac atgtccacaa tataagccag
     1021 agttgtgcca gtgtctatca cgtcctcgac cagcaggacg tctcgcccct cgatggaggt
     1081 atcgaggtcg gaaatgatcc gaaccgcccc cgtcgaccgg gtcgattcgt aggacgaaac
     1141 ccgcatgaat tcgatgtgat ggggaatggt gaggtggcgg atgaggtcgg ccaggaacac
     1201 cacccctcct ttgagaaggc tgatcaggac cgggtgccgg ctggaataat cgcgcgaaat
     1261 ctcccggccc aaccgggcca cgatgtcgtc gatctcggcc gcggtgacga ggacctggcc
     1321 gggcgacggg tggtcggggt ggttccggct cacgcgcggg tcgccttcat tcggacgacc
     1381 ctggcggtct ccggcaggac gcgaccgtcg tcgctcgtcg ccacccccgg cacccaatga
     1441 atcgtctcgc cttcgaccac gagcgcgcgc tcacgcgcgc gtctcggaac cttccgatcg
     1501 acgaagaggt cggagagctt ctttcgaccg tcgaggccga aggggcgcat gcgatcgcct
     1561 tcgcgaggtg cgcgcgcgac gagacgcgtg gagacggcat cggcgtcgaa ataggcggtg
     1621 gtggcatcgc gcgacgcgcc tgcgagctcg cggcgggcgg cggctgcgtc gatgaacgat
     1681 gtctcgatga gccagtcgcc ggcgcgggtg cggccgggga tggtcagcag cgccgcggga
     1741 cgttcggagg cggcttcacg gggcctgagc acaatcgcat cgtgctctcg ccgcgcacga
     1801 acgcgcggta aatcgaccga cgatcccgcg tgcgtgctcc gcaccagctc gagcaatcgc
     1861 tggtagtgca cacgacccac gtcgtgcgcc aagcccgcgt gcgcgagcgc gtcgcgcagt
     1921 agggcggtcg cgtcgtcgtc atcgagcgct tcgaaggcgt cgagtccgag cacccacacg
     1981 ccggactcct cttcgcgcac gacgcggcgc acgtcagtcc gggcctcgaa cgcgcgctgc
     2041 tcgccgcgcg cgccttcggc gatgcgcaac agcgcgtcgt cgatcgatgg gaacatcgcg
     2101 cgaagctccg gcaggatctg gtggcggacg cggttgcgcg caaactgcac gtcctcgttc
     2161 gtcggatcat cgacgaagac aacgccgcgc gacgcgcaat agtcgcgcgt gtcggcgcgg
     2221 gtaatgccga gcagcgggcg cacgaacgac ccccgccgcg ccggaatccc cgccaggccg
     2281 acgcggcccg cgccgcgcag gatgcgcatc aagacggttt cgacctggtc ggtttgggtg
     2341 tgcgccgtcg cgatgcacga cgcaccgagg tgcttcgcgg tggcggtcag gaattggtag
     2401 cgcgcgttgc gcgctgcttc ttcgatcgag gcgcgctccg cccgggcccg ccgcgccacg
     2461 tcttcgcttc ccaacacgaa ctccagccca tgcgcgcgcg cgtacgccgc cacgtgctcc
     2521 gcgtcgtgcg cactgtcttc ccgcaatcga tggtcgaagt gggcgacgac gggttgcacg
     2581 accgcgcgca atgcatcgac cagcgccatc gaatccgcgc cgcccgagac cgcgaccaac
     2641 acgactccgt cgcgcggcag caaggccgac gcgacgagcg cgcgctcgac cggatgttcg
     2701 agagggagga tcatgcgtgg actatatcac gcgaccgcgg agagaaaagg gtggtggcgg
     2761 agcagggatt cgaaccccgg acacgcggat tatgattccg ctgctctaac caactgagct
     2821 actccgccaa cccaatgtcg tgaaagaacg ccgcttagag atgaaagacc gcacgcgaga
     2881 acggcggccc tccaatgtac ccgagcgtcg caccggtgtc aaccagacgg cgtggaggcc
     2941 ggtcccgctg gcggagagca ccatcaccgg tccgcggggg agccgcgaag ccgggaaaca
     3001 cgttccgcag cgaccgcttg cttggggtcg atttcgagca ggcgctcata taccgcgatg
     3061 gcggccgcga cgtcgccttg ctgctcgcag gcgtcgccga cggcgagcgc gtcggtggcg
     3121 tgaatggtgg ggatgatcgc ggccgccttc agcgcgcaat cgctcgcccg cgcgagatcc
     3181 cccgcttgca agctcgcgcg tgagatcagt gaccacgccg cggcccgtcg cgggtccaac
     3241 gcaacggcgc gttcggccgc ggcgatgcac gcatcggtgc gtcccgccgc caactcgcgc
     3301 acggcgtcct cctgcaagcg ccgcgcctgg aagaacggct ggagcaggtg gtcgtatccg
     3361 ccggtgttca tcttccagac cacgcgtgca cgacgcccat cgaagatcgg gccccgcgtc
     3421 acgacccgca ccgcgtcgta gaactccgcg atgcgctcgt cctcgatcag gttttctccc
     3481 cgcaccagtg tcagcatgta gccggccggt atctcgcgcg cgaagtgccc gatcatccat
     3541 ttgataccgc gctcggggag ccgcgcgagc aacgggtcgg ccagcgcgta gccgtcgagg
     3601 atgtgggcgt ccgggcccga gtagtacccg aggaacccga ccatgccgcc gagttggact
     3661 gagcggccca cgccgcgcgc gccgcggcct tcggtcgcct tggggtggtc gacgccgcgc
     3721 ttgccgtccg cgcgaaacag gccggtgtgc tggaagtaga actcgcgctc gtcggcgatc
     3781 ccgcgaacca tggcgcgttt ctcgaggccg tggctgcgcc cgctcgtcac cgtcgggcgg
     3841 ccgggggcga gcccggtcag taccgccgcg gcggcggcgg gccaaagcac gcgccggctc
     3901 tgaaaccgaa actgcgacaa tacggccacg gccgcgaaga gcggcggcgt cagaaaccgc
     3961 ccgctcatga agtcgccgcc gatccacacg gtgtaggcga gatacgcggc gatgccgaag
     4021 acgaggggga cgagtcgctc gcgccggacc aggaacggga ccgcgatacc gaacgcgatg
     4081 acgacgagcg tcaagggatc acgtcgcagg gactcgataa gataatggac tccctgggtg
     4141 accagctccg agcgctggat gcccgtgttg agcttggcgt aggcggtgtt ggggacgagc
     4201 gacccgtagt agacgagcga gaacgtttcc cacgcgatga cgggcaggaa cgcgaccacg
     4261 gccgttttca gagccgcgcg cttggggagt cccttcgccg cgaccaccag cgcaggcccg
     4321 accaggaggg cgaagtccgc gcgcgtgaga attccgaggc ccgcgaccgc gcacaagtgg
     4381 aagaaccggc gaacgtcgcg cggcgtgcga aaccacgtta ggatgaacag cgcgagcaac
     4441 aggtgcgtca gcgggttttc gagcccgctg gtggcgtagt cgacgaaggc gcgcgacaac
     4501 agcagaatca agagtcccag cagcgcggcg ttcgtggtcc gtgctacacg gaacgcgagc
     4561 agcacgagcg ccgagagcga cacggcgatc gagagaacga tcccggtcaa gtacgcctcg
     4621 cgcgttacgg actggaacac gaggttcagg aacagccaca aggggtgcgt gtacgcctgg
     4681 acgcgttcgt ccgtgttcca ggtgagaccg tatccgtgga ggcaattgtc gaccacgcga
     4741 tacgtgatgt aggtgtcgtc cgtcacccag gccgtgcgca cgaggacgac ggaaaacagg
     4801 aacagggcgg cgacgaggag ccagcggcgc gacggccggc gaacggcgga ggccgcgttc
     4861 tgatcacgcg gagtgtcgcg cttgcggcgg ggtgtgacag acggtggcac gaagcggcag
     4921 tatacagcaa accgcattcc cctttccccc cgcgatgcga tcgactagat tcgcatcatc
     4981 cgcgaggcca gcgaactctc cggaggctaa acatgcgttt ccatgctgcg tcgtttgctc
     5041 tcgccgtcgt cgtcgcgcct cttttcgcca tggccgagtc ctcgcaaccc atgccgcccg
     5101 aagcctcgca attcgacttc tgggtcggcg aatgggacgc aacgtggggt gatcaacagc
     5161 gcggcatcaa tcgcgtcacc aagcgctggg acaccgtcgt ggtcgaagag ttcgacggaa
     5221 aacccggcct gcagctcgag ggtcacagcg tctcggtgtt cgacgccaac gcaaagcagt
     5281 ggaagcagac gtgggtcgac aaccaggggg gctatctcga tttcaccggc ggcttcgcgg
     5341 acggtcgcat gacgctctcg cgctcgttcc agaaagacgg ccagaccatc caccagcgca
     5401 tggtctggta cgacatcaca cccgatcgct tcgaatggaa ctgggagcgc tcgctggacg
     5461 ccggaaagac ctgggaagtc cagtggcaga tccactacgc gcgccgtaag tgaggcgagg
     5521 cgcgcggtgg tcggcgcgaa gtcccacccg tgcgcgccac ccgaaactac gctacaatac
     5581 gcggacgccc gcgccgggtg tccgcgatga acgtttacgc ccaccacgat ttgaagcaga
     5641 acctcctcga cggtgctacc gtcgcggtgc tgggtttcgg gaaccagggc cacgcgcacg
     5701 cgttgaacct gcgcgactcg ggggcccggg tggtggtggg cgcgcggcgc aacggggcgg
     5761 ggtggaaggc cgcgaaagcg gccgacttcg agacgctccc gatcggcgat gcgactgcgc
     5821 gtgccgatgt ggtcgtgatg ctgctcccgg acgaagagca ggcgagcgtg ttcgcctccg
     5881 aggtcgcgcc ctcgcttcgc gagggtgccg tcctggtctt cgcgcacggt ttcatggtcg
     5941 ccttcggcgg cttgaacacg gggagtagcc acgacgtcgt gctggttgca cccaaggggc
     6001 agggacactt cctgcgccgc gagtaccaag ccggccgctc ggtcccgtgc atggtcggcg
     6061 tcgaaaccga tatctccggc cgcgcgctgc agtgggcgct ctcgtacgcg tcgcacctcg
     6121 ggtgcttgcg ggcgggtgcc atcgcgacct cgtttcgcga ggaagcgatc accgacatgt
     6181 tcggcgagca ggcggtcttg tgcggtgggg tgccggcgct ggtcaaggcg gccttcgaga
     6241 cgctggtggc caagggctac tcgcccgagg tcgcgtacat cgagtgcctg caagagctca
     6301 agatcatcgc ggacctgatg tacgaaggcg gcctggccga catgcgcgcg cgcatcagca
     6361 agaccgcggc gtggggatcg ttcgaaagcg ggccgcgcct cgtgaccgac gccacgcgcc
     6421 gcgcgctcga gtcgatcctc gatgacatcg agtccgggcg cttcgcgagc caatggctgg
     6481 aggaagcgcg cgcgggccag cgtaccctcg ccgcgcgcat gcacgaggaa gaaacgcacc
     6541 cgatcgagaa ggcgggccgc gcggtgcgcg cgctcatgcg ccgctcggac ggcaaatcca
     6601 acaaggagtc gacgtgaagg gaatcaagat gggtcgaatc gggccgcgcc tggcggtggt
     6661 tgcgacggcg gcgttggcgc tcgcgagttg cagcgagaag gaggcggcgc gcgagaagcc
     6721 cgcggtcaaa cgctacaccg tccccgcgtc tctcgaccgc gagctgaaca acgcggagct
     6781 cgacagcttg agccggatga agcggaccca caacatcacg cgcgacgagt actgggatgg
     6841 gtacggcggt gtcctggcca acgacgtgct cgaagtgtgg tacccggacg gcaagctcaa
     6901 cgcgatgcaa ggcatggcga tgatgaagct cgcctccgaa gcgcgcgctc gcacgcagcg
     6961 ggtcttcggc gtggcacccg ccgagcgcgc ggtcatcatc tgcgcgggcg acatcgacgt
     7021 gtacacctct tcgaccggac gcgagtggtg gcactattcg cgcatcgtcg gcgacaccat
     7081 cagcatccag ccgcccatca atctgcacac gcgcgggatt ctcaccgtgg tggggccgcg
     7141 cgaatactac gagtgggcga tcggcaagat ctcgaagggg cacgcgccgc gctggatcga
     7201 agagggcctg gcgtcgtacc tggcgggcga agccgcggtg ctcgaggaac tgcgcgtcga
     7261 caccggcgac aaaccgatcg cgatgtcgac caaggacatg gagagcgccc tcgaaaaaga
     7321 aaacgaccgc caggagacgc ggcgcgctta ctacaacgcc taccgcacgg tcgagcagat
     7381 cgtgaaggcg cacggcgagg aagcgatggg acgcttcgtg gtggcgctgg gcggggacga
     7441 caacctcgac gccgcgtcgc aagccgcctt cggcgccaag ttcggcgagg tggccgaacg
     7501 cgcgcacgct tgggcgcgcg ccgagtagag aactacttga agagactccg gcgcggcacg
     7561 atcagccgcg cgaccaggag gccggtggcc agcgccacgc cggtcaggat catcgtgaag
     7621 gcggtgtgca gtccgctcac ggggtcgttg gcgatcatct cggtgatgct gcgaaagccg
     7681 atcgacccgg ggacgagcat gatggtgccc ggcgtagcgg tcacgatcgc cggacgattg
     7741 cgcgcgtgcg aaatgattcc gctcgtgacg cccaccagca ccgcgcccag gaacgccccg
     7801 ctctcgaccc cgatccactg cgcgcccaag cgcgacccca cgaaggcgag cacgcacacc
     7861 aggagtatcc accctccgtc gccgtagtgc gcttggttga gcaccatgaa cgcgagcgac
     7921 gccatcacga cggcgatggc gagcgtccac ggcgggagtg cttcgggatt gacgaccggc
     7981 accgacccga acagccgctc gccggcctcg agtcccaggg ccacgccgaa gccgatcgac
     8041 accaggatga tcaaggccga catcaagcgc gacgtgcccg actgcaagtg gcgcgtcgcg
     8101 agctcgctga tcgcggtggt cagcgtgagg ccgggcaaga gcacgatcag cccgcccgcc
     8161 atcgcgacgt agagcgaggt tggcggcatg agcgccgcgg ccgcatacgc gccgaatccg
     8221 ccgatgaacg ccgcgagggg ttcgaacaac cggaacctcg ggttgcgacg cgccaacagc
     8281 gccaacagac cgctcaccag gccgatcgcg ctgcacgcca ccatctcgcg ccagccgccg
     8341 ccgaagaagc acgcggaggc cgccgaggcc accgcgaacg cgatcgcggt ggtcttgccg
     8401 cggaagcggg gcggtgtggc gcgaatctcg tcgatgatgc gcgaggcttc cgcgggcgag
     8461 acccggccgt cggcgacgtc ctgcgtcaag cggtcgagac gcgaaagctt gtcgaggttc
     8521 accgagccgg gctcgacgcg aatcatgatg gtctgatcga tgtcaccggt gtcgagcgag
     8581 gcgaagatgg cggtcggggt ggcgaagaac tcgccgtgga tgcccagccg ctcggcgacc
     8641 aatgcgaggg catcctcgac cgtgggcgcg gccgagccgt agcggtgcag cgcgcgcgcg
     8701 agcttctcga tgaacgcgat cccttcgcgc ccgtgacgcg cgaccgggcg cgcgtcgggc
     8761 gcgtccgcca ggcgcatctc gtccaccacg cgcgcgtcgg ggcgcgggtt cgagcgccag
     8821 aaggactcgg cgattctgct cgtgaggcgt gggctcatgg tcgggccggg cttgcgcggt
     8881 cccggcgatg ctgctaaggt atagcatctt cgcgcgcgac gcaccgcgtt tgcgcgcacg
     8941 accgggtgga gggtcgtcta gtggcaggac aactgactct ggatcagttt gcgggggttc
     9001 gaatcctccc cctccaacca accttctatg cttcccgttg gcgccggtgc gccgcgccgc
     9061 gcgtcacgta gagcgcccag cccaccgccc ccagtcccag ccccacgatc acgaccagga
     9121 gcggccgaaa gcgccggacc agcgacgctc gctccgaggc gtcttcgagc gcggcgcgca
     9181 cgtcgtcggc gtcgtacatg acgccgtgca cgtagctcgc gaccttcaag tcggggctca
     9241 gcacgacgac caggttgggg tgcgcgaagc ccccctccgc gagcgccgtc accgtgaagt
     9301 cgatcgcgcc caggaaggtc gcgagatcgg cggcactcgc ggtcgcgagg cgccaacccg
     9361 ccgggagttc cagacgctcg cggtacgcgc gcatcgcggc ggaatcgtcg gcgggatcga
     9421 aggagatcgt caatacttcg tagcccacgc cgggctcgcc gatctcgcgc agcgcgtcgc
     9481 gcaggctctc ggtgatcatg ctgcacgtgt gcgggcagct ggtgaagatc gggctcacga
     9541 tgacgggctt gccccagagc gcgctcacgt gaaacgtgga gtcggcgtcg gacacgagtg
     9601 ccacgtcggg catctgggtc ccgatggtct tcttctcgtc ggggatcgtt tgggccgaag
     9661 ccatcgcggc ggcgagcgcc gccagcgcga gcgagtagac gacgcgtcgc attcggatca
     9721 gccggaatcg cgcgccaaca actcgcgctt cttgcgcgtc gcttccgcca tgtcggcgag
     9781 ggtcgtggtt tgcaggtaag tgcgaatgcg ttcgcgcagc ggcttccagc gctcgtgcag
     9841 cgggcacggc gtctcgtcgg agcagcgcgc gagtccgacc gcacaaccgt ccaagtaggt
     9901 cgtgccgtcg atcgcctgat aaatgtcgaa cagggtgatg gtcgagggtt cgcgcgcgat
     9961 cgagaaaccg cctccaggtc ctttgctcga gttcaggatg tcggcgcgca ccagcgtttg
    10021 gaatatcttg ccgagaaacg gacccggtac gcgctcgtgg gcggcaatgt cgcgcgccag
    10081 catgcgggtc ccgacgggag agccggccat gtgcgtcagc gcgcggatcg cgtattcgca
    10141 ggcgctcgag tggagcatcg acgtctcgat tgcggcttgg gtgcttgagt ttaggacccg
    10201 aatgtccggc ggccgcctgg aatctaaccg aggcggccat gtgctgtcaa cccatggggt
    10261 tcttgacact ccggggcgcc gatactattc tctcccgcac ggttgcggcc ccttcgtcta
    10321 gcggttagga cgtatggttc tcagccataa aacaggggtt cgattcccct aggggctacc
    10381 agttcttcgg ggacccgcgc ggcgcgggtc tctttttttc tgctcgttcc accggaaagg
    10441 acgacccgcg tcatggcaac tcgcacgacc ggccggcgcg cgcgcacgcg cacgccgagc
    10501 ggtccaaagt cgcgcgaccg gcactggctc taccaagaat cggtacaaag ccccgaggtc
    10561 cacttcgggt tcttcgagcg cgcgtatcgc gagcgcaccg gtcgccggcc gcgctcgctc
    10621 aaagaagact tctgcggcac cgcgctcttg gcggcgcaat gggtcgaagg ccaccgcgac
    10681 cgcaccgcga tcggcgtcga tttggacgcg cccacgttgc gatgggcaca gaagcacaac
    10741 atcgcgccgt tgtccgacga cgcgcgcgcg cgcatcacgc tgcgacgcga caacgtgatg
    10801 cacgtcgtta agcccaaggt cgatatcgtg gtggcgctca acttctcgta caacatcttt
    10861 catacccgtg aggcgctgcg cgcgtacttc gaggcggcgc ggcgatcgct cactccgggc
    10921 ggcgtgttca tcatcgacat gttcgggggt tgggattcgc agcggcccat gaccgaacgc
    10981 acccgccatc gcgggttcac atacctgtgg gagctgcacc actacgaccc ggtcgcgaac
    11041 ttcggccagt tctacattca cttcaagttc aacgacgggg gcggcattcg gcgcgcgttc
    11101 tcgtatgcgt ggcgaacctg gtcgatcccg gaggttcgcg aggtcctgct ggaggcgggg
    11161 ttcgaggacc tcgatctgta ttgggaaggc ttcgacacgc gcaccggttt gggcaacagc
    11221 gtgttccgca aggtcacgcg ggccaagaat tcgccgggct ggatcgcctt tttcgtggcg
    11281 tccgcatgag caaaaatggg gcatgacagg ggcgccgcgt tggcgcaaga tcgatgcacc
    11341 aaatccgccg tcgacgtccc gcggcctgcg ccgcggccac ggccaccgag gtaaagccat
    11401 gttcttccgc caggtctacg accccaagct ctcccagtac gcgtacgtca tcggttgcca
    11461 ggcaaccggc gaggccatcg tcgtcgatcc gcttcgtgac gtggaaagct atctcgagat
    11521 cgcgaagagc gaagggctgc gattggtcgc ggtggccgaa acccacatcc acgccgattt
    11581 tctctccggc gcgcgtgagc tgtgcgagcg cgcgggggtc accgcgtacg tctccggcga
    11641 aggcggggcc gactggcagt cggagtggct caagggaagt gcgtaccagc accgcgtgct
    11701 ccacgacggc gatcacttca tggtcggcaa cattcgcttc gacgtgatcc acacgccggg
    11761 gcacacgccc gagcacgtgt gctacaccgt gaccgacacg cccgcgcgcg cggcactcgc
    11821 ggtcggcatc ctctcgggcg acttcgtgtt cgtgggtgac gtcgggcggc ccgacttgct
    11881 cgagacggcg gccggacaag cgggcgcgat ggagcccagc gcacgcgcgc tgtttcgctc
    11941 catcgagaag ttccgcgcgc tcccgcccgc cctgcagctg tggccggcgc acggcgcggg
    12001 gagtgcctgc ggcaaggcgc tcggcgccgt gcccgtgtcg acggtcggct acgaactcgc
    12061 cgcgaacgcc tccatcggag cggccaccga ccccgaatcg ttcctcgcct acatcttgga
    12121 aggccagccc gagccgccgc tctacttcgc gcgcatgaag cgcgacaacc ggggcgggcc
    12181 cgcggtgctg ggcaagatgc cggaacccaa gccgctcgcc gcgagcaagc tcgtcgagct
    12241 cgagcgcgcc accggggtgg ccctgctcga cctgcgttcg tgggacgcgt acaagcgcgc
    12301 gcacgtgccg gggtcgctct tcacgccgct caaccgcgcg ttcaacacgg tggcgggctc
    12361 atacgtaccc gaaggcatgg cgatctacct gatcgtggac gagaagcgcg tgcgcgaggc
    12421 gaccgtcgat ttggtccgtg tcgggctcga tcgggtgatc ggctatgcga ccccggccgt
    12481 cttcgacgag tacgtgcgca aggggaagcc ggcgtcgatc gcggagatcg acgcgaccaa
    12541 ggtggaggct caactcgacg cgggcgcata tctgctcgac gttcgtcggg cggccgagcg
    12601 ggccgagcgc gggcagatcg cggggtcgca caacatcgcg tacgagcgct tgctcgcgcg
    12661 ggtgggcgag atcccgaagg accgcccggt ggtggtccac tgccagggcg gcacgcgctc
    12721 cgcgtacgcc gcggggctgc tcgatcgcat gggctattcg gtgacgaacg tcgccggcgg
    12781 ttacgacgca tggaagtcga aggccgaggt cgcgaagcgc taggggacga ccgcatgcaa
    12841 ttcgtcgtgt atggagccgg tgcggtgggg agcgtgctcg gtggcatgct atcgctccat
    12901 cgccacgacg tcctcctagt ggggcgcccg gcactcgtcg acgcggttcg cgagggcggg
    12961 ctgcggctca agtccgccac cggggagtac gtctcccacc cgcgcgtcgc cgcggcgctc
    13021 tccgcgggcg acgtgggagc cgacacccat gtcctcttca ccgtcaagtc gtacgacgtc
    13081 ccttcggccg tggccgcgct cgcgaaggtc gcgccgtcca ccagccccgt cgtgtgcttc
    13141 cagaacggca tcgatagcga agacgtggtc gcgaagcact tcgcgcgcgt gtacggcgcc
    13201 gtggtgcgca tgacgtgctc gatggtgcag ccgggccacg cgtcgtttcg cgcactgggc
    13261 aggctcgtgc tcggggcgca tcccaaggga accgacgccg tcgtggccga gctggtggcc
    13321 gcgttccgcg ccgccggctt cgacgcggcc gcgtccaagt cgatcgtcga cgacaagtgg
    13381 ctcaagctcg ccgtcaacgc gcagagcgtc gtccacgcgc tcgtcgacgc gcgcgaccag
    13441 gacgagaacg agttcttcga cttgaaggtc gccatcctgg aggagacgcg gcgcgtgtac
    13501 aaggcggcca aggtcaaggc gcgctcgggc gacggcaagg acccgtcgat cgacgagatg
    13561 atcgccgaga tcaagcgtcc acgcgcgcgg cgcaacgagc acggcgtcaa ggtgcacaac
    13621 tcggtgtggc aggacttcta tttgaagcgg ccgcgcatcg aggcggatgc gatccacggc
    13681 ccggtgatcg cgttgggcaa ggagcacgga gtcgccaccc cgtaccacga cgcgtcgctc
    13741 gcgctggccc ggcgctgcca cgtttccaag tccgctcccg agtcgatccg tctatcggaa
    13801 gtcctctccg agatcgcgaa acgacggtca tgaggacgat cgcgtacggc gcgtttctcc
    13861 tcctgctgct ggcggccagc gtcgtgctgt tgcgctcgat cgagcccgtc cacgaaggcg
    13921 cgggaccgtt tccgcgtgtg acgggctcgc tggagcccgt cgtgtcggcg atcgacctcg
    13981 atcgactgga aggcgcgggt gcgaccgagc tctacgacct cgcggcggag ttcatgcagg
    14041 tgtggcgacc gcgcgaggcg accatgctgc tggagcgcgc ggttgcggcc gactccacct
    14101 tctaccccgc ctggctcaag ctggtcgagt gttacgcgca cccgttggtg ggggacgagt
    14161 ctgcattgga gaaggcggtc gtgcgggccg ggacgaccgc tcccgcgccg gcggataccg
    14221 cactggtcgc cgggttgcgc cagctctacg tggaccgcga ctacgcgggt gccgtcggcg
    14281 cgctctccgc ggtggtgcgc gcggaggccg cgaccatcga cgcgcgctac cacctggcgc
    14341 tcgcgtacta ccagatgggg aggctgcgcg acgcgtcgcg ctacctcgaa ccgctcctgt
    14401 cggaagatcc gaccgtggcc ccggtggtcg aactctacat tcgccgcgcg gtcgcggctc
    14461 gcgagctcga gcgcgccgcc gacgccgccc gcgagctggc ccgcatgtac gcggaggagc
    14521 cgtttccgta cgtgttgagc gcgcaggtcg agctcgcgcg ggggaaccgg gaggccgcgg
    14581 tggagttttg cgacaacgcg ctcgatctgg accccgcgtg cgcgcccgcg atcatgacgc
    14641 gcgcgtgttt gtacgccgag gccggcgatt tccagtcggc gcgtgtgagc ttcgagaagc
    14701 tcctgctctt cgacgcggcc gccttgcagt cgatcggtca cgagggggtc gccttcgtgg
    14761 acttcctggc cggcgacttc gaccatgggg tggcttccat ggacgaagcg acgcgccacg
    14821 caatgttcgc gggtgccaca cgacggggcc tgtcgctcgc gtacggccac gtcgagtatc
    14881 tctgccaact cggacagccc gacgccgccc agggggtgat cgagcgctgg ctgacgggct
    14941 ttggtgagat ccccgtacaa atggcgagcg cccgtgtcgc gctcgcgcgc ggcgacaccc
    15001 gacgggccgc ggagacggtc gcacgcctgt cggccgagaa ggagtggatc ctgtgggcgc
    15061 gcaagctctc gctcgacgcc acggacctgg ccgcgcgcgt cgagatcggc gaagagcgcc
    15121 agcgtgaagc cgccgtacgc ctgcaggaag acgccaagtc cgcgaccgcg gtggcggcgg
    15181 gggcattcga gcggcgcacg ttcctcgccg gctacgccgc cttcgagagc ggagacgcgg
    15241 aatcggcggc ggccgcgttc agcgacgtgc accggcgcct gtacggcgtc gagtttccgt
    15301 atcacggcga cccggtcgtg tacgtgcagg cgctcttctt cctggccgag acggacctgg
    15361 cgcgcggcaa ccaggtcgcc gcgcgggcga gctacgaggc gtttctgggg tactggggag
    15421 atgcgtcgtg gaaactggaa gcggtcgacc gcgcccgccg caaactagaa gccctgggcg
    15481 aagcgaccgc ttcgccacag ggctagtcac ttgggccgac gcgtgggccg gtcagggctc
    15541 cctcgatcag gggaagatgc cgcgttccat atagatcttc tcgagacggc ccacggccag
    15601 cgccatggcc gcggtgcgcg gcgagcactt gcgcgcattc atctcgtcga tgacgccgag
    15661 ggtcgcgcgg gtcatgaccc ggttgagctc ctcgtccacc ttctcgagct cccagatctc
    15721 gctgttcttg ttctgcaccc actcgaagta gctcaccgtg acgccgcccg agttggcgag
    15781 aatgtcgggg atcacggtga tgccgcggga cgcgaggttc tcttccgcct ccggcgtcgt
    15841 cgggccgttg gcaccctcca gaacgaactt ggcctggacg taggagtcgt tgtcgacgtt
    15901 gacggtacac tcgagggcgg ccgggatgaa gatgtcggcc ttggtgtgat agaactcggt
    15961 cagcgcgatt tccttcgccc gcgggtagcc cttcacaccg cccttctgcg cgacgtacgc
    16021 cgccaggtcc tcggcgtcga taccgtccgc gttgccgatc gatccggtgt ggtcgttgac
    16081 cgacatgagg cgggagccgc gcgcctgcat gatacgcgcc gcgtgcgagc ccacgttacc
    16141 gaagccctgg acgaagaacg tcgacccctt gatctcgaag ccgacgtgct gcgcccacac
    16201 gtcgaagcag aacaccacgc cctggccggt ggccttgtcg cgccccaggc tgcccccgtt
    16261 gatgaggctc tttccggtga ccacgcggcg ggtctggttg cgctcggagg cgttgaacat
    16321 ctgcatgtag gtgtccatca tccagaccat ctcgcgcgcg ccggtgccga cgtcgggggc
    16381 cggaatgtcg tactcgggac cgatgttgtt gcccagggcg taggtgaagc gccgagtgat
    16441 gcgctggagc tccgccaacg agaagttgcg cgggttgaag ctcaccccgc ccttcgcccc
    16501 cccaaacgga atccccatga gcgccgactt ccacgtcatc cacgaggcga gcgccttgac
    16561 ctcggacagg ttcaccgact cgtggtagcg gatgccgcct ttgtaggggc cgttggtgtt
    16621 gttgtgctgg atgcgatagc ccttgaagag gcgatgggtc ccgtcgtcca tcgtcacggg
    16681 gaagttgacg atgatctcgt tctgcggctc cgacaggatg gtgcgcacca tcggattcag
    16741 gttgatgagg tcggcggcct tcctgaactg ggacgacgac atttcgaaca ggttcattcg
    16801 gtcactcata gggtctttct attcgggtct tgggaatggg tgattggctt cttggctcgc
    16861 ccgtgtcgtg cactcgcgca tcgtcgcggg cgggtctctg tcgagggaag tagtggcgca
    16921 gaacactatc agaaaacccg cgtgcgccgc aaccccgggg cgccccccga tccctggcgc
    16981 ccgcgatcca tcctcaagcc cggcaacgag atggcccggt gcccgggccc gtggccggcg
    17041 cccgtggcgt gacgcactat aaggagcgcc tgggatgctc gtccaagccc gggctccccg
    17101 atcatggcga ggaccgcagg ctccctcccc ggtcagatcc gcgcgcgcgt tccaaaggag
    17161 atcacgggac gttgcggaag ttcgcgtcga aagaacctcg cccccgcctt cgctcactcg
    17221 cccgacacgg cgacaccatc aacgggcgaa tcatcatcgc gacgatgttc gattcgttcg
    17281 tcgtgttgtg ccgagcaacg cccgccgcgc gcgcctcgac gcgtcgcatt gcgccgatga
    17341 gaacgccacg gaaacgcgtc gcgcgcgcga cgtgtgctgc gcacgcgcac gacgcatgaa
    17401 cccacgtgca cgacgcgcga cgtcccgcga atgatcacat tcggtcgtgc acatcgacga
    17461 tgcgcgaggg ggtcgcatga aagaattttt gccaccaacg ataatttttt attgacagcc
    17521 tttttcagtg tgcaagaatt cgcccaacga tgaaatcagc gatcgctgga tcattcgttg
    17581 aacgttggtt ggtctaaccc tgaacgaggc ggaggaagga tccttcgtcc gccaaggaag
    17641 gaaccaaaaa tggcagcaaa gaggaagtcc aaggccaaag gtaaggccaa gggcaaagcc
    17701 aagcgcaaag ccaagcgtcg ctccaagaag taggactatc ttggatcgca gcctggccag
    17761 gcagcgaatt cgcacaggga agcgagacgg cagtctcctt ctaacctttc ctggaggtaa
    17821 tagaaatggc aaagcgtaga gcaaaggcga aggcgaaacg caaggtgcgt cgcgccaagc
    17881 gtaaggtaaa gcgcaaagct aggcgcgtag tacgcaaggt ccgtcgcaaa gtgcgtcgca
    17941 aagtgcgtcg cgccaagcgc aaggtccgtc ggaaggtccg tcgccgcgcg gcagctgcgg
    18001 ccatggcgcc cgagatgtag ttccgggctc gcaagcccta gagctgcgaa tccgtgggaa
    18061 gggagacgtc agtctccctt ctcttttttt gtccccgctc ggccggccgg gcgaagggtg
    18121 gttgacaccg ccgcgcgatt ctcctatatg ttgcgcgact ttttctcgct tccacccatc
    18181 ccgagcggcg tcgacgcggc ccgtctcgat tccgaggaga acgcatggat ctggtcccgt
    18241 cggcaatgtt tctgaccaag ggcatgggcc gtcaccgcga gcagctcacc agcttcgagc
    18301 tggcactgcg cgacgccggc atcgcccacg tcaacctcgt caccgtctcg agcatcttcc
    18361 cgccgcagtg ccggctggtg cccaagcagc ggggcgccgc catgctccac cccgggcagg
    18421 tcgtgttcag cgtcatcgcc cgcatgtcga ccgacgaggc ctttcgtatg accgcgtgct
    18481 cgatcggggt cgccattccc ggcgatcgcg acaagtacgg atacctttcc gagcaccacg
    18541 cctacggcca gaacgagaag gccgccggcg actacgccga ggacctcgcc gccaccatgc
    18601 tggccacgac cctcggcatc gagctcgata ccgacaaggc gtggaaccag cgcaaggagc
    18661 agtggaagat cggccggcag atcgtgcgca ccaccaacat cacccagacc gcgatcggca
    18721 aggaaggttt gtggaccacc gtgatcgcgg cgggcgtgtt ctgcaagtac tagcccggct
    18781 cccatgactc tgggtttcct cgaggacaag gccgaccccg gcgacatcgt ccgcgacgag
    18841 aatcgctacg tcctggcgtt gctcccctac gagcagacca ccacctacgg gcagggagtg
    18901 ggccgagcgc cggaggcgat cgtccaggcc tcgagccatg tcgagctgtt cgacgaagtg
    18961 ctcgaggtgg acgcctcgcg ccacggaatc ctcaccgtcc ggccttcgat caccgacttg
    19021 gcctccgtca cgcgcgcggc ccggaagctg cgcgaggagc acccccattc actgatcgga
    19081 ttcgtgggag gcgagcactc gctgaccccc gcgttggtcg aagcgttcgc gccgcccgcc
    19141 accgggtcga agccgaatct cggcatcgtg tggatcgacg cccacgccga cctgcgccag
    19201 gaattccacg gccgcgagga caaccacgcc tgcgcgggtc acaactcgat gcgcttcggc
    19261 cccatcgtgc aggtgggaat ccgcacgctg gccgaagagg agtggtcgct cttgaagaag
    19321 accgaccgcg tccgcgccca tcgccactgg aacaagaagg cgcgcgccga cctcctggcg
    19381 ctccccaggg acgtctatct caccgtcgac ttcgacggct tctcgcccga ggtgattcgc
    19441 gcggtcggga ctcccgagcc gggcgggctc tattgggaag aggtcatgga catcctcgac
    19501 accgtgtttc gtcacaagaa cgtggtcgcg ttcgacgcgg tggaactggc ccccaacgac
    19561 gccgacatcg cgtcgtcgtt cacggccgcg cgcctggtct ataaggtgat gaactaccac
    19621 gccgcctaca cgctggggga ccgtcgcaag aaggcgcacc gggcgacgtc gtcctccgcg
    19681 cgcaagaagc accgacgcct cccgcgcaag cccaagcgaa gagcccgcac ggcgcgggcg
    19741 cgacgctcgc ggcggcggac gaaggcgcgt tcgacgcgga tgcggactac ttcgaccgcg
    19801 cgatgaaata gacgccgagg atgatgagga gaatgggaaa gacgggctgg tcgaggatgc
    19861 ccagcgagtg caagagccag atgatgccga gggcgaccag ggcgaggccc agtcgcgggg
    19921 aacctttctt cgtcgcaccc gtccccggcg cgcccgcgcc cgcttccgag gacgcacccg
    19981 agcctgtccc gcgcgcttcg ttcatcacgc cctccttgcc cttctcgtag gcgtcttcga
    20041 ggacgcgcac gccttccttg accgaaccgg ccacgcgctc ggcgacacgg tccagtcgtt
    20101 gttcccagtc tttctgttcc atgccgaaag cgtaaatcgc cgggcgggaa gtcgccaagt
    20161 ctaattccgc accgccggga tcttccgcga agccaccatc agaaccacga tgttcatcac
    20221 cagcaggaac accacgcccg cgaggaacac gtacaccatc tcgatcgagt aggcctgctt
    20281 gatgggcgcc acgaggtgca cggcccacgc gcccacgccg aaggtcagca tgaacttgac
    20341 cgcgtagctg gtgctgcgcc agcggtcggg cgtcatggcg gcgatcaggc tgttctcgat
    20401 gggctgcatg ccaaggctga agaactcgta ggcgaccgcg cacgcgacca gcacgacgtc
    20461 gcccgagaac gccatggcta ggacgaacgg gaagctggcc gcgtggaaca ggagatacgc
    20521 gcgacgcagc tccatgcggt cggcgagtcg cccgcccacg atttggccga acagcccgcc
    20581 gcataagacg atcgacgcca gcacggtggc cgcgaaggtc gccgtgctgt gtccgtcgac
    20641 gcgcggcaag tgctcgaaga gcttcgcgag aaacgtggtc ttgagctcca tgtaagcggg
    20701 caggagcagc atgtttcctc ggtacgcgat cccgcccagg acgagcgcca cgcacagaat
    20761 cccgaagtag cgcgccatct cgcgtctcgg cacgccgttg tggacgacgg gcttcgccgc
    20821 ggggcgaatg gtctcgtcga catggatggt ttgcaaccac gccgccgtga tcaagccgat
    20881 cgcgcccagc acgatcagcg ccgcctgcca cccgaacgcc cacgacaagg cgcccgtcgc
    20941 gagcggcgag agcgcgatgc cgaggttgcc gaacacaccg ttgacgccca tcgccacgcc
    21001 acgctggcgc acggtgcgcg agatgagcgc caggcccgcg gggtgataaa tactcgcgcc
    21061 caccccgacg aacgcgagcg acgccgtcat cacgcccggg ctcgggtagg cgcccgccac
    21121 gacgagcccc gcgcccatga tcgcgatgcc cgcgaccagc atcggcttgg cgcgccagcg
    21181 gtccgcggcg agtccgaccg ggagcgccaa gaggccgtag aggaggtaca tccagaagct
    21241 caatttgacg acggcctcga gcggcatcgc gaatgccgcc gtcagcggaa tcgtgatggc
    21301 gggaaatgcg agcgtgaaga agtgggtgaa gaaatgcccc gggcacgtga cgaggagaat
    21361 gcgatgctcc tcggggctga attcgctcat gcgacggaat ggcttcatgt ggatcgacgc
    21421 cgcggattca
//
LOCUS       DASPGK010000093         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1077193, whole genome shotgun sequence.
ACCESSION   DASPGK010000093 DASPGK010000000
VERSION     DASPGK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077193"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VEC56_02645"
     CDS             complement(<1..510)
                     /locus_tag="VEC56_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13080.1"
                     /translation="MSLSAPLIHVLSRLGIHQGLPAGTPTSPDDAREWSLATLAGRMV
                     EVSCDGAGASLTAATSLILEAQHHAEPAAWIEAGGSSFYPPDVAASGVDLNGLIVVRT
                     DATSKAARAADHLLRSGGFGLVILDLSHDASLAMSVQSRLSGLANAHRAAVVCLTRKK
                     SDALSIGSLV"
     gene            complement(526..741)
                     /locus_tag="VEC56_02650"
     CDS             complement(526..741)
                     /locus_tag="VEC56_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXV13081.1"
                     /translation="MKKPYDFTKAKKNPYARKLKRQVTIRLDEQTIEYFKKLAEEAGI
                     PYQTLINLYLRECAATGRRLSMTWKRD"
     gene            complement(744..1037)
                     /locus_tag="VEC56_02655"
     CDS             complement(744..1037)
                     /locus_tag="VEC56_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXV13082.1"
                     /translation="MAEIRFEWDEEKSRESRAKHGVSFEEAFTVFFDEEALLIPDAEH
                     SSTEERFIILGMSSTLRVLVVCHCYRRSSEVIRIISARKAFRVERRQYFSRWH"
     gene            complement(1184..1822)
                     /gene="lexA"
                     /locus_tag="VEC56_02660"
     CDS             complement(1184..1822)
                     /gene="lexA"
                     /locus_tag="VEC56_02660"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXV13083.1"
                     /translation="MSPKTPPGETRESIYQFMRDRLLDGKPPTVREVQARFGFKAVQT
                     AQAHLEQLVAEGRLAKEYGEYKGRARGYSLPESSVDYAKPTVFAPLLGHIQAGGLQAA
                     IEDREGVVPVQRGSRRAGSEQLFALRVRGESMTGIGILPDDVVVVRSQPSAEAGDVVV
                     ALVGDEATVKTLRKQRGQIVLQPENPSFEPIVLDPNDVTILGKVIEVRRYLD"
     gene            1995..2402
                     /locus_tag="VEC56_02665"
     CDS             1995..2402
                     /locus_tag="VEC56_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13084.1"
                     /translation="MIITRFAAVAGFLALSACDPLDSEDERPCVCTDQFVSVLFWAVD
                     SLQTPQDSFVVTVRNHRNGETYDVPQPTWGVGVYRAIDDSFTRRVSNGDPIQVTGERG
                     ARSFDAQFLIGVDDCQCHVHKISGPDTVVIENP"
     gene            2399..2869
                     /locus_tag="VEC56_02670"
     CDS             2399..2869
                     /locus_tag="VEC56_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13085.1"
                     /translation="MTSTSEDLWNHVIAQFACGVDSIHGPSHWRRVERDGLYIAERTG
                     AIVEVVTLFALFHDSRRENEDEDEGHGERGAAFAASLRGVMFDLPHEHFDLLHHACAR
                     HTDGETSDDPTVGACWDADRLDLGRVGIVPSADYMSTAVGRELAARGPSGRVIR"
     gene            2879..>3117
                     /locus_tag="VEC56_02675"
     CDS             2879..>3117
                     /locus_tag="VEC56_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13086.1"
                     /translation="MEPFIKAFIRASLLWFTGGIVLGVAIAIQPQWIVWRPAHAHMNV
                     VGFLTMLVFGVGYQLLPRLFGHPLHSQRLAVAHLYL"
BASE COUNT          545 a          977 c          975 g          620 t
ORIGIN      
        1 caccagcgat ccgatcgaca gcgcgtcgct cttcttacgc gtcaagcaca cgacggcggc
       61 ccggtgcgcg ttggccagcc cggagagccg gctctgcacc gacatggcga ggctcgcgtc
      121 gtgcgagagg tccaagatca cgagcccgaa cccgcccgag cgcaaaagat ggtccgcggc
      181 gcgcgcggcc ttcgaggtcg cgtcggtgcg cacgacgatc aatccgttca agtcgacgcc
      241 cgaagccgcg acgtcggggg gatagaagct cgacccgccc gcttcgatcc aggccgcggg
      301 ttcggcgtgg tgctgcgctt cgaggatgag ggacgtcgcc gccgtgagcg aagccccggc
      361 cccatcgcac gacacctcca ccatgcggcc cgcgagtgtc gcgagactcc actcgcgcgc
      421 gtcgtcgggc gaggtgggag tcccggccgg gagtccttga tggattccca gccgggacag
      481 cacgtggatc agtggtgcgg agagactcat gagaactcga ctcggttaat cgcgtttcca
      541 cgtcatcgac aagcgcctgc cggtagcagc gcactcgcgt aagtacaaat tgatcagcgt
      601 ctgatacggt attccggctt cctcggcgag ctttttgaaa tactcgatgg tttgctcgtc
      661 gaggcgaatc gtgacttgtc gtttcagttt gcgtgcatac gggttctttt tcgccttcgt
      721 gaagtcgtac ggtttcttca tcgtcaatgc cacctcgaga aatactgccg acgttcgacg
      781 cgaaacgcct tccgggccga aatgatccgg atgacctcgc tgctccggcg gtaacagtgg
      841 cagacgacca acacacgaag agtcgaactc atgccaagga tgatgaagcg ctcctccgtg
      901 ctcgagtgct ccgcatccgg gatgagcagc gcctcttcgt cgaagaagac cgtgaatgct
      961 tcttcgaaag agacgccgtg cttggcccga ctttctcggc tcttctcctc atcccattcg
     1021 aaacgtattt ccgccacacc ccgcctcccg ccaccctagt gtagttacag tgcaatgtca
     1081 atgcattgcc atagtattgc cttcgaaccg cggtcgcggc gtccctcgcc gtgtggtgca
     1141 gaagggaagg accggcgagg gacgcgcgcg cacacgtggt tggtcagtcc aaatagcgtc
     1201 tgacttcgat caccttgccc aaaatcgtga cgtcgttcgg atccaggacg atgggttcga
     1261 aactcggatt ctcgggttgc aatacaattt gaccgcgctg cttgcggagc gtcttgacgg
     1321 tggcttcgtc gccgaccagg gcgaccacga catcgcccgc ttcggcggac ggttgcgagc
     1381 gaaccaccac gacgtcgtcg ggaagaatcc cgatgccggt catgctctcg ccgcgcacgc
     1441 ggagcgcaaa gagctgttcg ctcccggcgc ggcggctgcc gcgctggacg gggaccacgc
     1501 cctcgcggtc ttcgatggcc gcttgcagac cgcccgcttg gatgtgaccc aacagcggcg
     1561 cgaagacggt cggcttcgcg taatccacac tcgactcggg gagcgagtag ccccgcgcgc
     1621 ggcccttgta ctcgccatat tctttggcga ggcgtccttc ggcgaccagc tgctcgaggt
     1681 gcgcctgggc cgtttgtacg gctttgaatc cgaacctcgc ctgcacttca cgaaccgtcg
     1741 gcggttttcc atcgagcagc cggtcgcgca taaactggta gatcgattcc cgggtttcgc
     1801 ctggaggagt cttgggagac atgttgcctt tcctcttcgg gcggctatca tgggtgcgtc
     1861 gccgtggcgc accaccccgt ccgacctgac gaaggacagt aaacagacaa ttgtcttttt
     1921 tggcaagagg gtttattttg gggtcttcga ggtggggagg cacgaggggt tggggccctg
     1981 agagaaatgt ggccatgatt atcacgcgct tcgccgccgt cgcgggattt ctcgccttga
     2041 gtgcctgtga tccgctcgac tcggaagacg agaggccttg tgtgtgcacg gaccagtttg
     2101 tcagcgtgct cttctgggcg gtcgattcgt tgcaaacacc gcaagacagt ttcgttgtga
     2161 cggttcgaaa ccatcgcaac ggagagacat acgatgtgcc gcagccaacc tggggtgtcg
     2221 gggtctaccg cgcgatcgac gattccttca cgcgacgagt ctcgaacggc gacccgatcc
     2281 aagtgaccgg cgagagaggc gcccgatcct tcgacgcaca gtttttgatc ggcgtcgacg
     2341 actgtcagtg ccatgtccac aaaatctccg ggccggacac ggtcgtcatc gagaatccat
     2401 gacatcgacc tccgaggacc tttggaatca cgtcatcgcg cagtttgcct gcggcgtcga
     2461 ttccatccac ggcccctcgc attggaggcg tgtcgagcga gatggcctct acatcgcgga
     2521 gcgcaccggc gccatcgtcg aggttgtgac gctgttcgcg ctctttcacg actcccgtcg
     2581 cgagaacgag gacgaagacg agggccatgg cgagcgcggt gccgcattcg ccgcgagtct
     2641 gcggggcgtc atgttcgatc tcccacacga acacttcgat ctcttgcatc acgcatgcgc
     2701 ccgccacacc gacggcgaga cgagcgatga tcccaccgtc ggcgcgtgct gggacgcgga
     2761 tcggttggac ctcgggcgcg tcggcatcgt gccgtcggcg gactacatga gcaccgcggt
     2821 cggccgggag cttgcggcgc gcggaccgag cggtcgcgtg atacgatagc cccgtacgat
     2881 ggaaccgttc attaaggcat tcatccgcgc ctcgctgctg tggttcacgg gaggcatcgt
     2941 gctcggcgtg gcgatcgcga ttcaaccgca gtggatcgtc tggcgcccgg cgcacgccca
     3001 catgaacgtg gtcggttttc tcaccatgct ggtgttcggc gtgggctacc agctcttgcc
     3061 gcggctgttc gggcacccgc tgcacagcca gcgtctcgcg gtggctcacc tctatct
//
LOCUS       DASPGK010000094         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1546154, whole genome shotgun sequence.
ACCESSION   DASPGK010000094 DASPGK010000000
VERSION     DASPGK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546154"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1969)
                     /gene="asnB"
                     /locus_tag="VEC56_02680"
     CDS             complement(77..1969)
                     /gene="asnB"
                     /locus_tag="VEC56_02680"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV13087.1"
                     /translation="MCGIVGVVALTERGQSWLDRLDAATERLALRGPDHGAVCRDRNV
                     GLGHRRLAVIDTSSAANQPMNDDSGRYTIVYNGEIYNFRELRDALVASGRTPRTHSDS
                     EVVLSLFADEGPSMLQKLNGFFAFAIYDRHERSLFLARDRLGIKPLLTYQDDDVLIFA
                     SELKAILSFPIRRELDDVSLFQYLQLSYVPAPHSMLKGVRKLEPGCTLTVREGAVRVE
                     RYYAVPTPTEKAALAYEDAQRRLSQVLDAAVAKQLVADVPLGAFLSGGLDSTVVAALA
                     ARHKPDLETYAIGFPDQPLYDESADAAAAAKALGVRHTTFPVTDDELYGHLFDVLDYT
                     DEPFSDSSALAVYILSRHTRRHVTVALSGDGADELFGGYNKHYAEALARRSRVRATLV
                     RAASPLWRSLPRSRETAFADAVRRLDRFARRAGLSPAERYWSWCSFTDQADVLRTLGG
                     RFQDLAASREYAARKGECTRWIDGRGINDVLYSDVALVLPNDMLKKVDAMSMANGLEV
                     RVPFLDHEVVEYAFSLPSHFKIDGGARKRILRDAFRGTLPAAVLRKRKHGFEVPLMRW
                     FRSHLCTLIRDDLLNRRFVEQQGIFDANEIDALVARLYSERPGDIAAQVWSLVVFQYW
                     WKKVMV"
     gene            2083..>2625
                     /locus_tag="VEC56_02685"
     CDS             2083..>2625
                     /locus_tag="VEC56_02685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV13088.1"
                     /translation="MQTELITIGITCFNAEGTIERAVQSARAQAWPSIEIVVVDDAST
                     DGSWAVVQRFAAGESRLRAFRHAANRGVGAARNSVLERAAGEFVAFFDDDDVSLPERL
                     FAQHRRIVEYERATGAPAVVCHTATEQRYPDGTTVYSPALGADATPGPSGDEVARLIL
                     LGQPTSGNRGVCPTSSQMARR"
BASE COUNT          434 a          878 c          907 g          406 t
ORIGIN      
        1 cacggctttc gctccgatcg ctccggcgct cctcccacgc tcgccattgg gttacgcgcc
       61 tccatcgccg cgcgcatcat accataacct tcttccacca atactggaac acgaccaggc
      121 tccacacttg ggccgcgata tcgcccggcc gctcggagta gaggcgcgcg accagcgcgt
      181 cgatctcgtt ggcgtcgaag atgccctgct gctcgacgaa gcggcggttc aagagatcgt
      241 cgcgaatgag ggtgcaaagg tgcgagcgga accaccgcat caggggcacc tcgaacccgt
      301 gcttgcgctt gcgcagcacc gcggcgggca gggtcccgcg gaaggcatcg cgcaggatgc
      361 gcttgcgcgc gcccccgtcg attttgaagt gcgacggcag cgagaaggca tactccacca
      421 cctcgtggtc gaggaacggc acgcgcacct cgagaccgtt ggccatcgac atcgcgtcca
      481 ccttcttgag catgtcgttc ggcaaaacga gcgcgacgtc cgagtaaagc acgtcgttga
      541 taccgcggcc gtcgatccag cgcgtgcatt cacccttgcg cgccgcgtac tcgcgcgacg
      601 ccgcgagatc ctggaaacga ccgccgagcg tgcgcagcac gtcggcttgg tcggtgaacg
      661 agcaccacga ccagtagcgc tccgcgggag acagccctgc gcggcgcgcg aatcgatcga
      721 gacggcgcac cgcgtcggcg aaggcggtct cgcgcgagcg cgggagcgat cgccacagcg
      781 gcgaagcggc gcgcaccaag gtcgcgcgca cccgcgaccg gcgcgccagg gcctccgcat
      841 agtgcttgtt gtagccgccg aacaattcgt cggcgccgtc gccggagaga gccacggtca
      901 cgtggcgccg ggtgtggcgg ctcaatatat agacggccag cgcggaggaa tcggagaacg
      961 gctcgtcggt gtagtcgagg acatcgaaga ggtgcccgta cagctcgtcg tcggtgacgg
     1021 ggaacgtggt gtggcgcacc cccagggcct tggccgcggc cgccgcgtcg gcgctctcgt
     1081 cgtagagcgg ctggtcggga aacccgatcg cataggtttc caagtcgggt ttgtgtcgcg
     1141 ccgccagtgc cgcgacgacg gtcgagtcga gtcccccgct caagaaggcg ccgagcggga
     1201 cgtccgcgac cagctgcttc gccaccgccg cgtcgagcac ttgcgacaat cgacgttgcg
     1261 catcctcgta ggcgagtgcg gccttttccg tgggcgtcgg caccgcgtag tagcgctcga
     1321 cccgcacggc accctcgcgc accgtgagcg tgcaccccgg ctcgagcttg cgcacgcctt
     1381 tcaacatgga atgcggggcc gggacgtagc tcaattgcag gtactggaac aaggagacgt
     1441 cgtcgagctc gcggcggatc ggaaaggaca ggatcgcctt gagctcggag gcgaagatca
     1501 acacgtcgtc gtcttggtag gtgaggagcg gcttgatccc caggcgatcg cgcgcgagga
     1561 acaacgagcg ctcgtggcgg tcgtagatcg cgaaggcgaa aaagccgttg agcttttgca
     1621 gcatcgaggg accctcgtcg gcgaagagcg agagcaccac ttccgagtcc gagtgggtgc
     1681 gcggcgtcct tcccgacgcg accagcgcgt cgcgcagctc gcgaaaattg tagatctcgc
     1741 cgttgtagac gatcgtatag cgcccgctgt cgtcgttcat gggctggttg gccgcgctcg
     1801 aggtatcgat gacggccagc cgccggtgcc ccagcccgac gttgcggtcg cggcacaccg
     1861 ccccgtggtc cggtccccgc aacgcgagac gctcggtggc cgcgtccaag cgatccagcc
     1921 acgattgtcc tcgctcggtg agcgcaacca cgccgacgat cccacacatg ggggcgaatc
     1981 ttagagcgaa cgtggccggt tcgccagggt ttccgccggg cggcaccgcg cgcccagagc
     2041 gcgcttggag gcggagcgag ggcgtgctag cctccgtccg gcatgcagac cgagttgatt
     2101 accatcggaa tcacctgctt caacgcggaa ggaactatcg agcgcgcggt gcagtccgcg
     2161 cgcgcgcaag cctggccgtc gatcgagatc gtggtggtcg acgacgcttc gacggatggc
     2221 tcgtgggcgg tcgtgcagcg cttcgcggcc ggggagtcgc gtttgcgagc gttccgccac
     2281 gccgccaatc gcggcgtggg tgcggcgcgc aactcggtgc tcgagcgcgc cgcgggcgag
     2341 ttcgtggcct tcttcgacga cgacgacgtg agcttgcccg agcgcctgtt tgcgcagcac
     2401 cgccgcatcg tcgagtacga gcgcgcgacg ggtgcgccgg cggtcgtgtg ccacaccgcc
     2461 accgagcagc gctatccgga cggcaccacg gtgtactctc ccgctctcgg cgcggatgcg
     2521 acaccggggc cgtcgggcga cgaggtggcg cgcttgatac tcctgggcca gccgacctcg
     2581 ggcaatcgcg gcgtgtgccc gaccagctcc cagatggcgc gccgc
//
LOCUS       DASPGK010000095         5956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1546666, whole genome shotgun sequence.
ACCESSION   DASPGK010000095 DASPGK010000000
VERSION     DASPGK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546666"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VEC56_02690"
     CDS             <1..708
                     /locus_tag="VEC56_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXV13089.1"
                     /translation="MALFGEKYGEEVRVVSIDGFSKELCGGTHVRRTGEIGAFFIRQE
                     TAVAAGVRRVEALTGEGALHVARQVMDDWSNIASLLRVAPHEVEKRVRSLLDENEALQ
                     KERKKSESKRAESGASEALASAVDAHGVRFVATTVEAPDVNALRSYGDALRGKLGLGV
                     ALVCQTGVEKPVCLIVSSDSAISERGVKADDLARRVATELGFKGGGKPHMAQFGIPSI
                     LEFERVRDVVKRALESA"
     gene            731..2227
                     /locus_tag="VEC56_02695"
     CDS             731..2227
                     /locus_tag="VEC56_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HXV13090.1"
                     /translation="MFFRIVSSAMILVMLATSVAPAQILQPRRTRDPFQQPTRSTTAA
                     PADTALVKESLRSIPQGFDAPVDPKTYVIGPGDQFVLFFRSTGQDIPLRVLPEGTVLV
                     PNAGMVRAAGLTIEAFRAELGRVLASYYRSTEFFCELVVPRTFVVYVLGEVAHPGPVA
                     MLPPYRVDTALTAAGGVTKSGSDRAIEIRKEGEPARFVDQLKFRRLGDVGMNPMLHEG
                     QTVFVPSRGRSCEVVGEVWRAGAYEILPGETVIDLIELAGGFTTNAERDELVLEQLSK
                     DEEVSIVKLDEATAATTVISDRDVIVVPDKRSFPGIDFVRVQGGGGRDGRIYLQEGET
                     LQSFLPRFIRLRNDFDLPHARIERKKGDGTFEFISIDLAKVLEGDSLDVELQTGDVIN
                     IPRLEDVVFVGGEVVRAGEIEFQRGLPAGRYIAMAGGPSDAGSVDKLQIYDSYGNRRD
                     GNRESVVYRGETILVKRRTGVILGNLFIGFVSLTSLFLSVYAVITANE"
     gene            2261..3325
                     /locus_tag="VEC56_02700"
     CDS             2261..3325
                     /locus_tag="VEC56_02700"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HXV13091.1"
                     /translation="MKALILAGGRGTRLRPITHTSAKQLVPIANKPILFYGIEAIRDA
                     GIEDIAIIVGDTADEVKHAVGDGSRFGARVHYIRQDAPLGLAHAVKISRDYMGQEPFV
                     MYLGDNLLSGGIRGFVEEFTRGKYDAQILLTRVKNPSDFGVAELKGDRVVGLEEKPKK
                     PKSDYALVGVYMFTPSIFEATEKIKPSWRNELEITDAIQYLIEHDYHVQSHVVEGWWK
                     DTGKLEDMLDANRMVLTGMATRVEMKQLEDSEFHGHVHLDDDVEVKRSILRGPIIIGK
                     GSRIVDAYIGPFTSIGPGSRVEKSEIENSIVLENCQIVDIGSRMTDSLIGRNVVVRRG
                     DKPPRAYRFMVGDNSEITVL"
     gene            3341..3925
                     /locus_tag="VEC56_02705"
     CDS             3341..3925
                     /locus_tag="VEC56_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXV13092.1"
                     /translation="MTRLADEMQKELAARAGLVASLDAEAARRMSAIATGMIACLRAG
                     GTVYACGNGGSATQAAHFAAELVGRYRKDRTALPAFALSENSAVVTSLSNDYSFDDVF
                     AHQIAGIGRPGDCLLALSTSGTSRNVVRACRAAADKGLHVFALTGAGGGEMARHSEIT
                     VAVPSTETPHVQELHLAILHILCRVVEDELFPAT"
     gene            3931..5178
                     /locus_tag="VEC56_02710"
     CDS             3931..5178
                     /locus_tag="VEC56_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXV13093.1"
                     /translation="MTVAALPRGVLVVSFFAFATFVPFSIAGMNIAFGFGALAWLASW
                     ASRDSARVPLHEDRLAWASLLLVASALPSVWMSENSARAIKDVSSYWQLLIVFWVGCN
                     VLAVRMRAASFWTLVVSSTLACCMVLVQGSSGLDLGFLDIPKRYRPGGTLYTMTFAGI
                     VYQLIVLNCAVLFTHAMRARNRIVLAAVVAIQIAAIMFTMTRGAWLALAAGLFALCVL
                     LRNRVLSWIATVLVVALAVFAVSAHKGRSIPELLRSGLDIDASTRVVLWDIAWDLFKQ
                     HPLTGVGMGDYTIEADKLLAGRRVTTTVDTHNVYLHILATRGVIGFVPFVLFWTVLVR
                     RLLRIRGSTEKGTADHQYAVGALAATVAVLVGALTELNIDDSEVFMAFMFVAGLALSP
                     SFAAGSRYDGGAAQRRKNDSQRS"
     gene            complement(5127..>5956)
                     /locus_tag="VEC56_02715"
     CDS             complement(5127..>5956)
                     /locus_tag="VEC56_02715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13094.1"
                     /translation="FVMGADKSIYAAARLAGVPLAAAERNSPEMYRMRLPAAAARFYF
                     ALFGLCRGIAVQFESYRRGYPPHLHDRIDVIPNAVREATRLAEPGRAAARHTLLSVGR
                     LSEQKRGDVLIDAFAALAARFPDWDLRIVGEGPERPTLEAHVRAHGLAGRVALPGATA
                     AVEDEYARAHAFAVASRWEGFPNALAEALAHGLPAVGFAACPGVNELIEPGSNGLLAA
                     GVDDANALATTLAGLMSDAETRRSMGERARASMAAYHGEAVYDRWESFFRRCAAPPS"
BASE COUNT          968 a         1922 c         2062 g         1004 t
ORIGIN      
        1 atggcccttt tcggcgagaa gtacggcgag gaggtccgcg tggtctccat cgacggtttc
       61 tcgaaggaac tatgcggcgg cacgcatgtt cgacgcaccg gtgagatcgg cgccttcttc
      121 attcgccagg agaccgcggt cgcggccggc gtgcggcgcg tcgaagcgct gaccggcgag
      181 ggcgcgctcc acgtcgcgcg tcaggtcatg gacgattggt cgaacatcgc gtctctgctg
      241 cgcgtggcac cccacgaggt cgagaagcgc gtgcgttcgc tgctcgacga gaacgaagcg
      301 ctgcaaaaag agcgcaagaa gagcgagagc aagcgggccg agagcggcgc cagcgaagcg
      361 ctcgcgagtg cggtggacgc gcacggagtt cgcttcgtcg cgaccacggt cgaggccccc
      421 gacgtcaatg cgctgcgcag ctacggcgac gcgctgcgcg gcaagctggg gttgggcgtc
      481 gccttggtct gccagaccgg ggtggagaag cccgtgtgcc tcatcgtatc gagcgactct
      541 gccatcagcg aacgcggtgt gaaagccgac gacctcgcgc gccgggtggc gaccgagctg
      601 ggcttcaagg gcggcggcaa gccccacatg gcgcagttcg gcatcccgag tatcctggaa
      661 ttcgagcgcg tgcgcgacgt cgtcaagcgt gcgctcgagt cggcgtgacg ttccgtcgta
      721 cgaggagagt atgttttttc gaatcgtatc ttccgcgatg atcctggtca tgctcgccac
      781 ttcggtggcg ccggcgcaga tcctgcagcc gcgccggacg cgcgacccgt tccagcagcc
      841 gacccggtcg accacggccg cgccggcgga caccgctctg gtcaaggaat cgcttcggtc
      901 gattccgcag ggcttcgacg cgcccgtcga ccccaagacc tacgtgatcg gacccggcga
      961 ccagttcgtc ctcttcttcc gttccaccgg acaggatatc ccgctgcgcg tgctgccgga
     1021 agggaccgtg ctggtcccca acgcgggtat ggtgcgcgcg gccggactga ccatcgaggc
     1081 ctttcgcgcg gagctcggac gggtgttggc ctcttactat cgcagcacag aattcttctg
     1141 cgagctggtc gtgccgcgca cgttcgtggt gtacgtgctg ggagaggtgg cgcaccccgg
     1201 tccggtggcg atgctcccgc cgtatcgcgt cgacaccgcc ctcaccgccg cgggcggtgt
     1261 gaccaagagc ggcagcgacc gcgcgatcga gattcgcaag gagggcgagc cggcgcgttt
     1321 cgtcgatcaa ctgaagttcc ggcgtctggg tgacgtcggc atgaacccca tgctgcacga
     1381 gggccagacg gtgttcgttc cctcgcgcgg gcgttcgtgc gaggtcgtgg gtgaggtgtg
     1441 gcgcgcgggt gcgtacgaga ttctgccggg cgagacggtg atcgacctca tcgaactcgc
     1501 gggggggttc acgaccaacg ccgagcgcga cgagctggtg ctggagcaac tctcgaagga
     1561 cgaggaggtg tcgatcgtca aattggacga ggcgaccgcg gcgaccaccg tcatcagcga
     1621 ccgcgacgtg atcgtggtgc cggacaagcg cagcttcccc gggatcgact tcgtgcgcgt
     1681 ccagggtggc ggcggccgcg acgggcgcat ctacttgcag gagggcgaaa ccttgcagtc
     1741 gttcctgccc cgcttcattc gcctgcgcaa cgacttcgac ttgccgcacg cgcgcatcga
     1801 acgcaagaag ggtgacggta ccttcgagtt catttccatc gacctggcga aggtgctgga
     1861 aggggattca ctcgacgtcg aactccagac cggtgatgtg atcaacatcc cgcggttgga
     1921 agacgtggtg ttcgtggggg gcgaagtggt acgggcgggc gagatcgagt tccagcgcgg
     1981 gcttccggcc ggccgctaca tcgcgatggc gggcggcccg tcggacgcgg gcagcgtcga
     2041 caagctgcag atctacgaca gctacgggaa ccggcgcgac ggcaaccgcg agagcgtggt
     2101 gtaccgaggc gagacgattc tggtcaagcg acgcacgggc gtcattctcg gcaacctgtt
     2161 catcgggttc gtcagcctca cgagcctgtt cttgtcggtg tacgcggtga tcacggccaa
     2221 cgagtgacgg cgcacgccgc gaggaaggaa gggatcgacc atcaaagcgc tcattctcgc
     2281 cggtggacgg gggacgcggc ttcgtcccat cacgcacacc agtgccaagc agctggtgcc
     2341 catcgccaac aagccgattc tcttctacgg catcgaagcg atccgcgacg ccggcatcga
     2401 ggacatcgcc atcatcgtgg gtgataccgc cgacgaagtg aagcacgcgg tgggagacgg
     2461 ctcgcgtttc ggcgcgcgcg tgcactacat cagacaggac gcgcccctgg gcctcgcgca
     2521 cgcggtcaag atctcacgcg actacatggg ccaggagccg ttcgtcatgt atctgggaga
     2581 caatctgctc tcgggcggca tccgcggttt cgtggaagag ttcacccggg gcaaatacga
     2641 cgcgcagatc ctcctcacgc gggtcaagaa cccgagcgac ttcggcgtgg ccgaattgaa
     2701 gggcgaccgt gtggtgggtc tcgaagagaa acccaagaag cccaagagcg actacgcgct
     2761 ggtaggggtg tacatgttca cgccgtccat cttcgaggct accgaaaaaa tcaagcccag
     2821 ctggcgcaac gagctcgaga tcaccgacgc catccagtac ctgatcgagc acgattacca
     2881 cgtgcaatca cacgtggtgg agggatggtg gaaggacacc ggcaagctcg aagacatgct
     2941 cgacgcgaat cgcatggtgc tgaccggcat ggccacgcga gtcgagatga aacagctgga
     3001 ggattcggag ttccacggcc acgtccacct cgacgacgac gtcgaagtca agcgcagcat
     3061 cctgcgcggt ccgatcatca tcggaaaggg ctcgcgcatc gtcgacgcct acatcggtcc
     3121 cttcaccagc atcggcccgg ggtcgcgggt ggagaagagc gagatcgaga acagcatcgt
     3181 gctcgagaac tgccagatcg tggacatcgg cagccgcatg accgacagcc tgatcgggcg
     3241 caacgtggtg gtgcggcgcg gcgacaagcc cccgcgcgcc taccgtttca tggtgggcga
     3301 caacagcgag atcacggttc tgtaacgccg gacgatccag atgacacgat tggccgacga
     3361 gatgcagaag gaactggccg cgcgcgcggg gctggtcgcg tccctcgacg ctgaggccgc
     3421 gcgtcgcatg agcgccatcg cgaccgggat gatcgcgtgt ctgcgggccg gcggcacggt
     3481 ctacgcgtgc ggaaacggcg gcagcgccac ccaggccgcc cacttcgcgg ccgagctggt
     3541 cggccgctac cggaaggacc gcaccgcgct gcccgcgttc gcgctctccg agaacagcgc
     3601 ggtggtgacg tcgctctcca acgactattc cttcgacgac gtgttcgcgc accagatcgc
     3661 cggcatcggg cggcccgggg actgcctgct cgcgctctcg accagcggga cctcgcgcaa
     3721 cgtggttcgc gcgtgccgcg ccgccgcgga caagggcctg cacgtgttcg cgctgaccgg
     3781 cgcgggcggg ggcgagatgg cacggcacag cgagatcacg gtggcggttc cgtccaccga
     3841 gacgccccac gtgcaagagc ttcacctcgc gatcctgcac atcctgtgtc gcgtggtgga
     3901 agacgaactc tttcccgcca cctagccgtg atgacggtcg cggccctccc tcgcggcgtc
     3961 ctggtcgtct cgttcttcgc attcgcgacc ttcgttccct tttcgatcgc ggggatgaac
     4021 atcgccttcg ggtttggcgc gctggcgtgg ctcgcctcgt gggcgtcgcg cgactcggcc
     4081 cgggtccccc tgcacgagga ccgcctggcg tgggcctcgc tcctgctcgt tgcttcggcg
     4141 ctcccgtcgg tttggatgtc cgagaactcg gcgcgcgcga tcaaggacgt ctcgtcgtac
     4201 tggcagttgc tgatcgtctt ctgggttggg tgcaacgtgc tcgcggtgcg catgcgcgcg
     4261 gcgtcgttct ggaccctggt ggtctcgtcg accctggcct gttgcatggt cttggtgcag
     4321 ggatcgagcg gactcgatct cggttttctc gacattccca agcgctaccg cccgggcgga
     4381 acgctctaca ccatgacctt cgcgggaatc gtttatcaac tgatcgtctt gaattgcgcg
     4441 gtgctgttca cgcacgcgat gcgagctcgc aatcggatcg tgctggcggc ggtggtggcg
     4501 atccagatcg cggccatcat gttcacgatg acgcgcggcg cgtggctcgc gctcgcggcg
     4561 ggtctattcg ccctgtgcgt gctgctgcga aaccgcgtgc tgtcgtggat cgcgaccgta
     4621 ctcgtggtgg cgctggcggt gttcgcggtc agcgcccaca aggggcgctc gattcccgag
     4681 ctgttgcgtt cggggctcga catcgacgcc tccacgcgcg tcgtgctgtg ggatatcgcg
     4741 tgggacctgt tcaagcagca cccgctcacg ggtgtgggga tgggcgacta caccatcgaa
     4801 gcggacaagc tactggcggg gcggcgcgtc accaccaccg tcgacacgca caatgtctat
     4861 ttgcacatcc tggcgacgcg gggtgtcatc ggattcgttc cgttcgtctt gttctggacc
     4921 gtgctcgtgc ggcgcttgct gcgcatacga ggcagcaccg agaagggcac cgcggaccac
     4981 cagtacgcgg tgggtgcgct tgcggccacc gtcgcggtgc tggtcggcgc gctgaccgag
     5041 ctcaacatcg acgacagtga agtcttcatg gcgttcatgt tcgtcgcggg gctggccttg
     5101 tcgccctcgt tcgcggcggg aagtcgctac gacggcgggg ccgcgcagcg gcgaaagaac
     5161 gactcccagc ggtcatagac cgcctcgccg tggtaggcgg ccatcgacgc gcgcgcgcgt
     5221 tcgcccatcg agcgcctcgt ctccgcgtcg ctcatcagcc ccgcgagtgt ggtcgcgagt
     5281 gcgttcgcgt cgtcgacccc ggccgctagc aatccgttcg atcccggctc gatcaattcg
     5341 ttgacgcccg gacaggcggc gaatccgact gccggaagcc cgtgcgcgag tgcttcggcc
     5401 agcgcgttgg gaaagccctc ccagcgcgag gcgacggcga acgcgtgcgc gcgcgcgtac
     5461 tcgtcctcga cggccgcggt cgcgcccggc agcgcgacgc ggcccgcgag cccgtgcgcg
     5521 cggacgtgcg cctcgagcgt ggggcgctcg ggcccctcgc ccacgatgcg caggtcccaa
     5581 tccgggaagc gcgcggcgag tgccgcaaac gcgtcgatca gtacgtcgcc gcgtttctgc
     5641 tccgagagac gccccaccga gagcagcgtg tggcgcgcgg ccgcgcgccc cggctcggcc
     5701 agcctggtcg cttcgcgcac cgcgttgggg atgacgtcga tgcggtcgtg caggtgtggc
     5761 gggtagccgc gccggtacga ctcgaactgc accgcgatgc cgcgacacag cccgaagagc
     5821 gcgaagtaga agcgcgcggc ggcggccggt aaacgcatgc ggtacatctc gggcgagttg
     5881 cgctcggccg cggcgagcgg caccccggcg agccgggcgg cggcgtagat ggacttgtcc
     5941 gcacccatca cgaagc
//
LOCUS       DASPGK010000096         5939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1546749, whole genome shotgun sequence.
ACCESSION   DASPGK010000096 DASPGK010000000
VERSION     DASPGK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546749"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VEC56_02720"
     CDS             <1..698
                     /locus_tag="VEC56_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13095.1"
                     /translation="FLEQDKPYMSTTHAHNAVESLNEATIEMLRSAQSCSQGQGGSQS
                     SAQQMLQQMIPQQQDVIKETQAMMQMRLTEEALRQQRQAQLDRLAGQQRSLQDIAKRI
                     EESAKGNREKLGSLERTIEEMEAVVEALERGAVDEDLVNKEQRILSRLLDAERSVHTR
                     DYEKRRESVSAEDVFSKSLGRRPEGADAQTLRDEIQRAMQLKAPGEFEDLIRMYFRAL
                     ADEAPAPAAPRSP"
     gene            700..2619
                     /locus_tag="VEC56_02725"
     CDS             700..2619
                     /locus_tag="VEC56_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13096.1"
                     /translation="MRVRFVIAATLGTAIAVASVFAPVLAQEQRPRQPFAADPLSVSG
                     DVLEATRLLAVNPPAGIALLQQLNARYPGRDDILARLGYGMQVVGKPDSAAFYYRAAL
                     QANPLNLDAGKSLGSIYFSSGKEREAMQIFDRLLEANNYSMGAYKMVAGALRDLGRID
                     EAISVLEEGRGKSKKNAGLSLEIANLYKQSGNPKRAMDEYFSYAATDARAYRFAREKM
                     LEILNDAGPDEGAIVAYLRTRADARGTGAFAASDVLAAYYLQKGQLESSLEMALRADE
                     DQAADGASLLVLTEESLSRAETQPRNSRARYLDLALRSSESYVRNHPNSPGLDRAMFA
                     LAGIYAEYGSGANPALPAAERTAYLERAVAQYADVSKRFGGSDLAERAYVARGDVLLY
                     DLKRPDAALDAYRSGAVNSRRLGGEFAGRIARVFIGTGRTKDAEHYIASLSRAGNPEL
                     AQAGQYYTGLYLATQKKYELARDTLTALAEGAPSSAFTNDAIEAAWVIEEGLMLKSGS
                     LDDFVAAEKAAMVGDTTAIVSKYQAIAGREIHDPLRPRALHRLGLLYFDQGAFDTALA
                     TLQGFLKDYPKEDDCPAVTRDVGRVYEVGLGDYNQALKEYEKILLSYPEYAMLDDVRR
                     DVQRVRSLVKGSYAP"
     gene            2609..3865
                     /locus_tag="VEC56_02730"
     CDS             2609..3865
                     /locus_tag="VEC56_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HXV13097.1"
                     /translation="MRRSLVALAFLVATALPASAQQLLVPMDLTQSNHLKAYGLAFTA
                     LSKGHKIDWLLNYRGGSFVMPASADIVLDARLKNVSFVEVSGADVAKIHAEIAQNNME
                     VVHLETAPKVAVYIPPLKQPWDDAVTLALEYAEIPYARVFDEEVLANGLEKYDWLHLH
                     HEDFTGQYGKFYAAYRHTDWYQAEQRDAEARARAMGFSKVSEVKKAVAEKIREYVMSG
                     GFLFAMCSACDSYDIALAANGVDIVAQEFDYDGLTPGFQDKLDYERCFAFTGFHLVPS
                     PLVYEFSDIDTSDYVSARGSDADYFTLFEFSAKYDPVPTMLTQNHVGYVKGFLGQTTG
                     FKKALVKKSVVILGEDAVHDEVKYLHGNAGRGTFTFLGGHDPEDYQHAVGDPATDLAL
                     YTTSPGYRLILNNILFPAAKKKTLKT"
     gene            3884..4372
                     /locus_tag="VEC56_02735"
     CDS             3884..4372
                     /locus_tag="VEC56_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HXV13098.1"
                     /translation="MDATVWLWILAGALVVAGFVGVIAPVLPGAPLVLAGLIVAAWAE
                     DFQYVGWPAITILAILTAITYIVDAVASALGVKRVGASKQAVWGAVLGTTVGVFFGIP
                     GILLGPFVGAVAGELLVTRDAKQLGRAGVAAWIGFIIGTIGKLALMFTMVGVFIVARL
                     LI"
     gene            4380..5342
                     /locus_tag="VEC56_02740"
     CDS             4380..5342
                     /locus_tag="VEC56_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXV13099.1"
                     /translation="MRRNAIVLLVVLVVCAAGAEVFFRSRAASERERIRSRMGERGLC
                     TQPADDERLIYTYTPGECGANSLGYRDIEHSIEKPEGVFRIVLIGDSVAEGRDVEADS
                     AFGRVIERQLNARGDGRRYEVILLAHIGYSTSQEIVLLHDTAFRYDPDLILWSYCLND
                     PAHPVYHNANGDLGRYYHRPRSYLVARVEAFAFRVKQKYRGLECPKEFHAFVHCAFWP
                     EVEENLGRIGEITLSHRVPVVFVIHPIFEKDTGFEDYSLRDVHARLAEAARTNGFDVV
                     DLLEPFRAYAPAGLKIENETYYDPWHMNALGHRVAARAIAPHIP"
     gene            5502..5693
                     /locus_tag="VEC56_02745"
     CDS             5502..5693
                     /locus_tag="VEC56_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13100.1"
                     /translation="MEKVFPGCQKCNKGLLLPLSDYGRDGAPITYKAWVCNNPDCGFN
                     IRIDNGEISVGRDISTSVK"
     gene            complement(5705..>5939)
                     /locus_tag="VEC56_02750"
     CDS             complement(5705..>5939)
                     /locus_tag="VEC56_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13101.1"
                     /translation="IYHVTGNLAYTIVAHAIFNSVAFAQLAFQTEEQASQLPVYLSDV
                     RIVVGALVIFIFLLFKTKEGGPETEPPYESLDQ"
BASE COUNT         1056 a         1936 c         1960 g          987 t
ORIGIN      
        1 tgttcctgga gcaggacaag ccttacatgt cgaccacgca cgcgcacaac gcggtggagt
       61 cgctcaacga ggccaccatc gagatgctgc gcagcgcgca aagctgctcg cagggacagg
      121 gcggaagcca gtcgagcgcg cagcagatgc tgcagcagat gatcccgcag cagcaggacg
      181 tgatcaaaga gacgcaggcc atgatgcaga tgcgcctcac cgaggaggcg ctgcgccagc
      241 agaggcaggc gcagctcgac cgcctggccg ggcagcagcg ctcgctgcaa gacatcgcga
      301 agcggatcga ggaatcggcc aagggcaacc gcgagaagct gggcagcctc gaacgcacca
      361 tcgaagagat ggaggccgtg gtcgaagcgc tggagcgcgg cgcggtcgac gaggatctgg
      421 tcaacaaaga gcagcgcatt ctgagccggc tgcttgacgc cgagcgctcg gtgcacacgc
      481 gcgactacga gaagcggcgc gagagcgtgt cggccgaaga cgtgttctcg aagagcctgg
      541 gacggcggcc cgagggcgcc gacgcgcaga ccttgcgcga cgagatccag cgcgcgatgc
      601 agctcaaggc gccgggcgag ttcgaggacc tcattcgcat gtacttccgc gcgctcgccg
      661 acgaagcgcc ggcgccggcc gcgccgcgga gcccgtagcg tgcgcgttcg cttcgtcatc
      721 gccgcaaccc tcgggaccgc gatcgcggtc gcgagtgttt tcgcgcccgt gctcgcgcaa
      781 gagcagcgcc cgcgccagcc cttcgccgcc gacccgctga gtgtcagcgg cgacgtgctc
      841 gaggcgaccc ggctcctcgc cgtcaatccg cccgccggca tcgcgctcct tcagcaattg
      901 aacgcgcgtt accccggccg cgacgatatc ctcgcgcgcc tgggctacgg catgcaggtg
      961 gtcggcaagc ccgactcggc cgcgttctac taccgcgcgg cactccaggc caacccgctc
     1021 aacctcgacg ccggcaagtc gctgggctcg atctacttct cgtccggcaa agagcgcgag
     1081 gcgatgcaga tcttcgaccg cctgctggaa gcgaacaact acagcatggg cgcctacaag
     1141 atggtggcgg gcgcgctgcg cgacctggga cgcatcgacg aggcgatctc ggtgctcgaa
     1201 gaggggcgcg gcaagtccaa gaagaacgcg gggctctcgc tggagatcgc gaacctctac
     1261 aagcaatcgg gcaatcccaa gcgcgccatg gacgagtact tctcgtacgc agccaccgac
     1321 gcgcgcgcgt accgcttcgc gcgcgagaag atgctcgaga tcttgaacga cgccggcccg
     1381 gacgaaggcg ccatcgtcgc ctacttgcgc acgcgcgccg acgcgcgcgg taccggcgcc
     1441 ttcgccgcgt cggatgtgct ggcggcgtac tacctgcaga agggtcagct ggagagctcg
     1501 ctcgagatgg cgctgcgcgc cgacgaggac caggccgccg acggcgcgtc gctcttggtc
     1561 ttgaccgagg agtcgctctc gcgcgcggag acgcagccgc gcaactcgcg cgcgcgctac
     1621 ctcgacctcg cgctgcgctc gtcggagtcg tacgtgcgca accaccccaa ctcgccgggc
     1681 ctcgatcgcg ccatgttcgc gctggccggt atctacgccg agtacggctc gggcgcgaac
     1741 cccgcgctgc cggccgcgga gcgcaccgcg tatttggagc gcgcggtcgc gcagtacgcc
     1801 gacgtctcga agcgcttcgg cgggtcggac ctggccgagc gcgcgtacgt cgcgcgcggc
     1861 gacgtgctgc tctacgatct caagcgcccc gacgcggcgc tggacgcgta ccgctcgggt
     1921 gcggtcaact cgcgccgcct gggcggcgag ttcgcgggcc gcatcgcgcg cgtgttcatc
     1981 gggaccgggc gcaccaagga cgcggagcac tacatcgcgt cgctctcgcg cgccggcaac
     2041 cccgagctcg cgcaggccgg ccagtactac accggtctct atctcgccac ccagaagaag
     2101 tacgaattgg cgcgcgacac gctgaccgcg ctggccgagg gtgccccgtc gtccgcgttc
     2161 accaacgacg ccatcgaggc cgcgtgggtg atcgaagagg gcctcatgct gaagtcgggc
     2221 tcgctcgacg acttcgtcgc cgcggagaag gcggccatgg tgggcgatac caccgccatc
     2281 gtgtcgaagt accaggccat cgcgggccgc gaaatccacg acccgctgcg cccgcgcgcg
     2341 ctccatcgac tggggttgct gtacttcgac cagggtgcgt tcgacaccgc gctcgcgacg
     2401 ctgcagggtt tcctcaagga ctatcccaag gaagacgact gccccgcggt cacgcgcgac
     2461 gtcggtcgcg tgtacgaagt cggtctcggc gattacaacc aggcgttgaa agagtacgag
     2521 aagattctgc tgtcgtaccc cgagtacgcc atgctcgacg acgtgcggcg cgacgtgcag
     2581 cgcgtgcgct cgctcgtgaa gggatcgtat gcgccgtagt ctcgttgcac tcgcgtttct
     2641 ggtcgcgacg gcgctgcccg cgagcgcgca gcagctcttg gtcccgatgg acctcacgca
     2701 gtcgaatcat ttgaaggcgt acgggctcgc gttcacagcc ctctccaagg ggcacaagat
     2761 cgactggctg ctcaattata ggggcggcag tttcgtgatg cccgcgtcgg ccgatatcgt
     2821 gctcgacgcg cgcttgaaga acgtcagctt cgtggaagtg agcggcgccg acgtggccaa
     2881 gattcacgcc gagatcgcgc agaacaacat ggaggtcgtc cacctcgaga cggcacccaa
     2941 ggtggcggtg tacatcccgc ccttgaagca gccctgggac gacgcggtga cgctggcgct
     3001 cgagtacgcg gagattccgt acgcgcgcgt gttcgacgaa gaggtgctgg cgaacgggct
     3061 cgagaagtac gactggctcc acctgcacca cgaggacttc accggccagt acggcaagtt
     3121 ctacgccgcc tatcgccaca ccgactggta ccaggccgag cagcgcgacg ccgaggcacg
     3181 cgcgcgcgcc atgggattca gcaaggtctc cgaagtgaag aaggcggtcg cggagaagat
     3241 tcgcgagtac gtgatgagcg gcggattcct gttcgcgatg tgctcggcgt gtgactcgta
     3301 cgacatcgcg ctggcggcca acggggtcga catcgtggcg caggagttcg actacgacgg
     3361 cttgacgccc ggcttccagg acaagctgga ctacgagcgc tgcttcgcgt tcaccgggtt
     3421 ccatttggtg ccgagcccgc tcgtgtacga attctccgac atcgacacca gcgactacgt
     3481 ctccgcgcgc ggcagcgacg ccgactactt cacgctgttc gagttctcgg ccaagtacga
     3541 ccccgtgccc accatgctga cgcagaacca cgtgggttac gtgaagggtt tcctcggaca
     3601 gacgacgggg ttcaagaagg cgctggtcaa gaagagtgtc gtcatcctgg gcgaagacgc
     3661 ggtgcacgac gaggtcaagt atctccacgg caacgcgggc cgcgggacgt tcacgttcct
     3721 cggcggtcac gatcccgagg actaccagca cgcggtcggt gacccggcca cggacctcgc
     3781 tctctacacc acctctcccg gctaccggct catcctcaac aacatcctct tcccggccgc
     3841 gaagaagaag acactcaaga cctagtcgcg ctatcctctc tccatggacg ccaccgtctg
     3901 gctctggatt ctggccggcg cgctggtcgt ggccggcttc gtcggtgtca ttgcgcccgt
     3961 gctgccgggc gcgccgctgg tcctcgcggg attgatcgtg gccgcgtggg cggaggactt
     4021 ccaatacgtg ggctggccgg ccatcacgat tctggcgatc ctcaccgcca tcacgtacat
     4081 cgtcgacgcg gtcgcgagtg ccctgggtgt caagcgcgtg ggcgcgagca agcaggcggt
     4141 gtggggtgcg gtactcggga ccaccgtggg cgtcttcttc ggtattcccg gcattctact
     4201 cggaccgttc gtgggcgcgg tcgctgggga gctgctcgtc acgcgcgacg ccaagcaact
     4261 cggccgcgcg ggggtggcgg cgtggatcgg cttcatcatc ggcacgatcg gcaagctcgc
     4321 tctcatgttc acgatggtgg gtgtcttcat cgtggcgagg cttctgatct aagcggcgta
     4381 tgcgtcggaa cgcgatcgtc ttgctcgtcg tcttggtcgt gtgcgccgcc ggcgccgaag
     4441 tcttctttcg ctcccgcgcc gccagcgagc gcgagcgcat ccgatcgcgc atgggcgaac
     4501 gcgggttgtg cacccaaccc gcggacgacg agagactcat ctacacctac acgcccgggg
     4561 agtgcggcgc caattcgctc ggttatcggg acatcgagca ttcaatcgag aagccggaag
     4621 gcgtcttccg catcgtgttg atcggcgact cggtcgcgga ggggcgcgac gtggaagccg
     4681 actcggcatt tgggcgcgtg atcgagcgcc agctcaacgc gcgcggcgac gggcgtcgct
     4741 acgaagtgat cctcctggcg cacatcggct attcgacctc gcaggagatc gtgttgttgc
     4801 acgacacggc cttccgttac gatcccgatc tcattctatg gagctattgc ctcaacgacc
     4861 ccgcgcaccc ggtctatcac aatgccaacg gcgacctcgg acgctactac caccgcccgc
     4921 gctcgtatct ggtcgcgcgc gtcgaagcct tcgcgttccg cgtgaagcag aagtatcgcg
     4981 ggctcgagtg cccgaaagaa ttccacgctt tcgtgcactg cgccttctgg cccgaggtcg
     5041 aagagaatct cgggcgcatc ggagagatta cgctgagcca ccgcgtgccg gtcgtcttcg
     5101 tcattcatcc catcttcgag aaggacaccg gcttcgagga ctactcgctg cgcgacgtgc
     5161 acgcgcgcct ggcggaagcg gcgcgcacga acggcttcga cgtggtcgat ttactggagc
     5221 cgtttcgcgc gtacgcgccc gcgggcctca agatcgagaa cgagacctac tatgacccat
     5281 ggcacatgaa cgcgctcggt catcgcgtgg ccgcgagggc catcgcgcca catatacctt
     5341 gacaccctcg gcgagtcgcg cggataatta tgcggtcacc cctgacagtg ctgtaacatc
     5401 ttcattcgca tcgagttgat ggagaagtcg gtgggtcggg ggtcggtgat gaaagggagg
     5461 tgacgaccca agcgcgaacc cccctcgtgc tgcgggcgag aatggaaaag gttttcccgg
     5521 gatgccagaa gtgcaacaag gggctcttgc tgcccctctc cgattacggc cgcgacggtg
     5581 cgcctatcac gtataaggcg tgggtctgta acaacccgga ttgtggattc aatattcgaa
     5641 tcgacaacgg ggagatcagc gtcggacgcg acatcagcac gtccgtgaaa taatacgccg
     5701 cgcgctactg atcgagcgac tcgtagggag gctccgtttc ggggcctccc tctttcgttt
     5761 tgaagagcag gaagatgaag atcacgagcg ctcccaccac gatgcggacg tcgctcaagt
     5821 aaaccgggag ctggctggcc tgctcctcgg tttggaacgc aagctgcgcg aaggcgacgg
     5881 aattgaaaat ggcgtgcgcg acgatggtgt acgcgagatt tccggtcacg tggtagatg
//
LOCUS       DASPGK010000097         8208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1312297, whole genome shotgun sequence.
ACCESSION   DASPGK010000097 DASPGK010000000
VERSION     DASPGK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312297"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VEC56_02755"
     CDS             complement(<1..797)
                     /locus_tag="VEC56_02755"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXV13102.1"
                     /translation="MTTYYLTTAIDYVNARPHLGTAYEKIGADCLARYKRLAGFDTYF
                     LMGTDEHSTNVEREARNLGKDPQAYTDEMALVFEHTWKTLHISYDQFIRTTDARHKRA
                     VAKLFETIHRNGFIYEGKYSGLYCEGCEEFKAEKDLMDGKCPRHLTVPKFIEESNYFF
                     ALSKFSDRLKSHILAHPDFVRPEIRKNEILNVIESGLEDVSVSRAGKTWGVPLPIAPD
                     QVVYVWFDALINYISAIGYAADGADDKRLFERYWPCDVHVIGKDITR"
     gene            899..1690
                     /locus_tag="VEC56_02760"
     CDS             899..1690
                     /locus_tag="VEC56_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXV13103.1"
                     /translation="MDWLTDPQVWVSLATLTALEIVLGIDNIIFIAILSMKLPAHQQD
                     RARFVGLAIAMITRVALLFSLTWMMKLTNPLFGVFGRDFSGRDLILIGGGLFLLAKST
                     LEIHEKLEGHEDEHHATFKRATFGVVILQIVLLDIVFSLDSVITAIGLSEHLPVMVTA
                     VVIAVIFMMAFAKGISAFVDKHPTIRMLALSFLILIGVSLVGEGFHFHIPKGYIYFAM
                     AFSVLVEMLNLRLRKVSEPVALHGEIEASKRAAQTVAPPEPSGDA"
     gene            complement(1687..2787)
                     /gene="corA"
                     /locus_tag="VEC56_02765"
     CDS             complement(1687..2787)
                     /gene="corA"
                     /locus_tag="VEC56_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HXV13104.1"
                     /translation="MSRRSRRARARAKPPRLFKRHHAGKVLPPGTLVYDREPRVAQVS
                     IQVMDYGPDACRVRQPKTIEDTFPLRDSTSTSWIDIEGLHDTELLNKFGAHFGLHPLV
                     LEDILNTHQRPKLEEYENYLYVVLRMLAMRNGDALHSEQVSIIVGRGFVATFQEIPGD
                     VFDPVRKRIEQGGGRSRRMGSDYLAYALMDAIVDNYFALLEKTAETIEEVEQRVGDKP
                     RPDDLAKIHELRREMVYLRRNVWPLREVVAALQRSESDLIHDETRVYFRDLHDHVVQV
                     IESLENFRDVLASLQDLYVSGIGVRTNEVIRVLTIISTIFVPLTFLAGVYGMNFDVLP
                     ELRWPHGYLYFWIVSVIVVISLIVFLRRRGWY"
     gene            complement(2756..2989)
                     /locus_tag="VEC56_02770"
     CDS             complement(2756..2989)
                     /locus_tag="VEC56_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXV13105.1"
                     /translation="MRTVKTIIIVLLFALLLIVMVQNAQPVTFNFLNWRYQVSQLLLV
                     VIVFVIGWLVGFVMGKVTGKRKEDEPPLSPRPR"
     gene            complement(3010..3675)
                     /locus_tag="VEC56_02775"
     CDS             complement(3010..3675)
                     /locus_tag="VEC56_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXV13106.1"
                     /translation="MSKPPRRARKTKKPDAPAPKTKAPMNRVFAHVRRFVIRGTLALI
                     PIALPAFAVYLLYGFIDRRLLVLVHKTFGISFPGMGIVLLLVILYLFGLVVSNFFGRQ
                     LLQGLESLTERIPIINTAYRIGKQLSGTLSLPEKQIFRRPILVPYAAPGTWQVGFVTG
                     TVRSERDGVELLKVYVPTPPNPTSGFVYFMKESDTRDPGWTVEEAMQCILSGGLIGSP
                     TIR"
     gene            3725..4498
                     /locus_tag="VEC56_02780"
     CDS             3725..4498
                     /locus_tag="VEC56_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HXV13107.1"
                     /translation="MPIVWQWVLFHVLLAIALWIDLGVVHKRPESVTTKRALSWVGVW
                     MALAFLFCAGVWVVDGRHDAVLFLTGYLVEQSLSVDNLFVFWVIFQYFHVPRKLEHRI
                     LFWGIVGALVFRLSFILAGVALLERFHWLIYVLGAFLVVTAARLMFAKDRQVHPEKNP
                     VFKFLKPFMGTQSYDSGKFFMRKDGRLMITPALVVLLVIETTDIVFAVDSIPAILAIT
                     TDPFLVYTSNAFAILGLRSLYSAIGKLIHTFEFLHHGLA"
     gene            4522..4653
                     /locus_tag="VEC56_02785"
     CDS             4522..4653
                     /locus_tag="VEC56_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13108.1"
                     /translation="MLISHWYKMPAGVTLVVVITVLTSTVVSSIWLRKIKESRRKAS"
     gene            complement(4625..5749)
                     /locus_tag="VEC56_02790"
     CDS             complement(4625..5749)
                     /locus_tag="VEC56_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13109.1"
                     /translation="MPPRASQEPRPLPALDLEQPQASAYLRSLKRGAIPPLLFDGPEG
                     TGKEFTAIEFARALQCEKDEPCRLDGPRCASCLHASILEHPGIHLVYPTPTQGSNEDD
                     EGDVGDIAKILEEKRADIFAAPTFTKKTSIRVARARAVIQRAFTKPFDARYHVFIFVD
                     AHAMREEAQNALLKLVEEPPAHAAIVFVTPNAEGLLYTIRSRCQRVRFFPVKRAVLEK
                     ILISYYNADAQVAKRAAALAQGSIVRARALLDSADFAERDAALGLVAGLAKEKESWAL
                     SQALIGARGANRESVARVLDEMAVIFRDAMTGDASLYVNADVAKDIERVGAVWGSAKL
                     PRAIESILETRAQVFLANANIDGALAELFLKLKRLSAATP"
     gene            complement(5794..6972)
                     /locus_tag="VEC56_02795"
     CDS             complement(5794..6972)
                     /locus_tag="VEC56_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXV13110.1"
                     /translation="MMRPLRAVLLTAALAAALTTVVAWAQTDDVDEKQAELDRIRREI
                     EEHRAKSQKLGSQEKQVLKKLSAVDKDIDLSKRYLKKLTEQESTIDNRIGDLKVMIGG
                     REIVLSQQEEALGKRLRQMYKRDPQYTWEVFLGAQSIDDAITRYQFMKLIASQDANLI
                     GEFKESKKSLEIETARLAESLQEVWELREARERETAQLQHSKKQRQTVLAGIRSEKSK
                     HAKAIEDLEAAQAELQALIDDIIARGTAGKDLPPTGEFAAMKGRLLWPVAGKIIRGFG
                     KHTHPKYGTVTMNNGVDIQAPAGAPIVAVATGVVEFVDWIDAFGKCVILNHGGGFYTL
                     YAHVATTMVAQGQKVGRGQAIAEVGDTGSLEGYICHFEVRRGRLAMNPAEWLGKKQGS
                     "
     gene            complement(6969..7838)
                     /locus_tag="VEC56_02800"
     CDS             complement(6969..7838)
                     /locus_tag="VEC56_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXV13111.1"
                     /translation="MMRRILYIVDEALRTVRRHKGLTSISVVIMSLSLLMLAVFLLAT
                     DNLLKLVSETQEEMKIYVYLDDNVGQAHIEELYRSILATPEVSSVQFVSKEEALVDFK
                     GQLGDDAELLSALRANPLPNSFWIEPKAEHKSRDAMVGLAERFGHMRGVEEVRYGKEF
                     LDKFDSIVKGIYTIDIVVGFIVIMSAIFIIANAVRLTVISRRKTIDILKLVGATNPFI
                     VAPFIIEGAFQGGVAAVLSLLLLRVVTEVSRSAIPDINFFSVEKSLVFFLTCVLIGAL
                     GSFMALRKYLRMQ"
     gene            complement(7846..>8208)
                     /locus_tag="VEC56_02805"
     CDS             complement(7846..>8208)
                     /locus_tag="VEC56_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV13112.1"
                     /translation="QQRVAIARALVNHPAILLADEPTGNLDPDSAEDVLRLLFKINNG
                     GTAVLMATHDHSFVQNFGQRIIHLRDGKVYEDKTTGLVGNLSDRLDALRMERDRARRV
                     NDAIKRQRTTSLAPTKTE"
BASE COUNT         1393 a         2584 c         2609 g         1622 t
ORIGIN      
        1 aagcgcgtga tgtccttgcc gatgacgtgg acgtcgcagg gccagtaccg ctcgaagagg
       61 cgcttgtcat cggcgccgtc ggccgcgtat ccgatggccg atatataatt gatcagcgcg
      121 tcgaaccaga cgtacaccac ctggtcgggc gcgatcggca gcgggacgcc ccaggtcttc
      181 cccgcgcgcg acacgctgac gtcctccagc cccgactcga tcacgttcag gatctcgttc
      241 ttgcgaatct cgggtcgaac gaagtccggg tgggcgagga tgtgtgactt caagcgatcc
      301 gagaacttag agagcgcgaa gaagtagttg ctctcctcga tgaacttggg gaccgtgagg
      361 tgacgcggac acttcccgtc catcaggtct ttctcggctt tgaattcttc gcaaccctcg
      421 cagtaaaggc cgctgtactt cccctcgtag ataaacccgt tcctgtggat cgtctcgaag
      481 agtttcgcta ccgcgcgctt gtggcgcgcg tccgtcgtgc gaatgaactg gtcgtacgaa
      541 atatggagcg tcttccaggt gtgttcgaac accagcgcca tttcgtcggt ataggcctgc
      601 ggatccttgc ccaggttgcg cgcttcccgc tcgacgttgg tcgaatgctc gtcggtcccc
      661 atcaggaaat aggtgtcgaa gcccgccagg cgcttatagc gcgcgaggca gtcggcaccg
      721 atcttctcgt acgccgtgcc caggtgcggg cgcgcgttga cgtagtcgat ggcggtcgtg
      781 aggtagtagg tggtcattcg ggattcgctg gcgaaaaatg acgctagaat gtagcattcg
      841 ggctccgcgc tcgcaagcgc ggacccggtg aggggaatcc caaggagaag tcactggtat
      901 ggactggctg accgacccgc aggtgtgggt gtcgctcgcg accctgaccg cgctcgagat
      961 cgtcctcggc atcgacaaca tcatcttcat cgccatcctg tcgatgaagc tgcccgcgca
     1021 ccagcaggac cgcgcccgct tcgtgggcct cgcgatcgcg atgattaccc gcgtggcgct
     1081 gctcttttcg ctcacgtgga tgatgaagct caccaacccg ctgttcgggg tgttcggccg
     1141 cgacttctcg ggccgcgacc tgatcttgat cggcggcggc ctgtttctcc tcgccaagag
     1201 caccctcgag attcacgaga agctcgaggg ccacgaagac gagcaccatg cgaccttcaa
     1261 gcgcgcgacc ttcggcgtcg tcatcctgca gatcgtcctg ctcgacatcg tcttctcgct
     1321 cgactcggtc atcacggcca tcgggctctc ggagcatctc cccgtgatgg tgaccgcggt
     1381 cgtcatcgcg gtcatcttca tgatggcgtt tgcgaagggg atcagcgcct tcgtcgacaa
     1441 acacccgacc atccgcatgc tggcgctgag cttcttgatc ctgatcggcg tgtcgctggt
     1501 gggcgagggc ttccacttcc acatcccgaa gggatacatc tacttcgcga tggcgttctc
     1561 ggtgctggtg gagatgctga acctgcgcct gcgcaaggtg agcgagccgg tcgctctgca
     1621 cggcgagatc gaagcgtcga agcgcgccgc gcaaaccgtc gcaccgccgg agccgtccgg
     1681 cgacgcctag taccagccgc gccggcgcaa gaagacgatg agcgagatca cgacgatcac
     1741 gctcacgatc cagaagtaga gatagccgtg cggccagcgc agctccggca gcacgtcgaa
     1801 gttcatgccg tacacgcccg cgaggaacgt gagcggtacg aaaatggtcg agatgatggt
     1861 gagcacgcgg atcacttcgt tggtgcgcac cccgattccc gacacgtaca ggtcctgcaa
     1921 gctcgccagc acgtcgcgga agttctccag cgattcgatc acctgcacca cgtggtcgtg
     1981 gaggtcgcgg aagtagacgc gcgtctcgtc gtgaatgagg tcggactcgc tgcgctgcag
     2041 ggcggccacg acttcgcgca gcggccacac gtttcggcgc aagtagacca tctcgcgacg
     2101 cagctcatgg atcttcgcca ggtcgtcggg gcggggttta tcgccgaccc gctgctccac
     2161 ttcttcgatc gtctcggcgg ttttttccag caaggcgaag tagttgtcga cgatcgcgtc
     2221 catgagcgcg tacgcgaggt agtcggagcc catgcggcgc gagcgaccgc cgccttgctc
     2281 gatgcgcttt cgcaccgggt cgaacacgtc gcccgggatc tcctggaacg tcgccacgaa
     2341 accgcggccg acgatgatgc tcacctgctc ggagtggagc gcgtcgccgt tgcgcatcgc
     2401 cagcatgcgc agcaccacat agagataatt ctcgtattcc tcgagcttgg gacgctggtg
     2461 ggtgttgaga atgtcttcga gcacgagtgg gtgcagcccg aagtgcgcgc cgaacttgtt
     2521 caacaactcg gtgtcgtgga gtccttcgat atcgatccac gacgtggacg tcgaatcgcg
     2581 cagcggaaag gtgtcttcga tggtcttcgg ctgcctcacg cggcacgcgt cggggccgta
     2641 gtccatgacc tggatggaga cttgggcgac acgcggctcg cggtcgtaga ccagcgtgcc
     2701 gggcgggagc accttgccgg cgtggtgccg cttgaagagc cggggcggtt tggccctagc
     2761 gcgggcgcgg cgagagcggc ggctcatctt ccttcctctt gccggtgacc ttgcccatga
     2821 cgaaccccac caaccatccg atcacaaaca cgatcacgac cagcaacagt tgcgacactt
     2881 gatatcgcca gttcaggaag ttgaacgtca ccggctgcgc gttctgaacc atcacgatca
     2941 acagtagagc gaagagaagc acgattatga tggtcttcac ggttcgcatc gatttacctc
     3001 cgttcgtcgt caccgaatcg tcggtgaccc gatgagtccg cccgagagaa tgcactgcat
     3061 ggcctcttca acggtccagc cggggtcgcg ggtgtcggat tccttcatga agtagacgaa
     3121 ccccgacgtg gggttgggcg gggtgggcac gtagaccttg agcagttcca cgccgtccct
     3181 ctccgagcgc acggtgcccg tgacgaatcc cacctgccac gtgccggggg cggcgtacgg
     3241 caccaggatc gggcggcgga agatctgctt ctcgggcagc gataacgttc ccgacagctg
     3301 cttgccgatt cgatacgccg tattaatgat ggggatgcgc tcggtcaggc tctcgagccc
     3361 ttgcagcagc tgccggccga agaaattgct gacgacgagc ccgaagaggt acaggatcac
     3421 cagcaagagg acgatgccca tgcccgggaa gctgatcccg aacgtcttgt gcaccagcac
     3481 gagcaagcgc cggtcgatga agccgtagag caagtagacc gcgaaggccg gcagcgcgat
     3541 gggaatcaac gccagcgtcc cgcgtataac gaaccgccgc acgtgcgcga acacgcgatt
     3601 catcggcgct ttggtcttgg gagcgggagc gtcaggcttc ttggtcttgc gcgcacgtcg
     3661 ggggggcttg ctcatcgggt tcactctacc aaatccgcgc cgcgggtgtt aacgtcttct
     3721 gcctatgccc atcgtctggc agtgggtcct cttccacgta ctcctggcca tcgcgctgtg
     3781 gatcgacctc ggcgtcgtcc acaagcgccc cgaatccgtc accaccaagc gcgcgctgag
     3841 ctgggtcggg gtgtggatgg cgctggcgtt cctgttctgc gcgggcgtct gggtcgtcga
     3901 cggccggcac gacgcggtct tgttcctcac cggctatctg gtcgagcagt cgctcagcgt
     3961 cgacaacctg ttcgtgttct gggtgatctt ccagtacttc cacgtgccgc gaaagctcga
     4021 gcaccgcatt ttgttctggg gcatcgtggg cgcgttggtg ttccgtctct cgttcatcct
     4081 ggccggggtc gcgctgctcg agcgcttcca ctggctgatt tacgtgctgg gcgcgttcct
     4141 ggtggtgacc gcggcgcgtc tgatgttcgc gaaggaccgc caggtccacc ccgagaagaa
     4201 ccccgtgttc aagttcttga agcccttcat gggcacgcag tcgtacgact cgggtaaatt
     4261 cttcatgcgc aaggacgggc ggctcatgat cacgcccgcg ctggtggtgc tgctcgtgat
     4321 cgagaccacc gacatcgtgt tcgcggtcga ctcgatcccc gccatcctcg ccatcacgac
     4381 cgacccgttc ttggtctaca cgtcgaacgc gttcgccatc ctggggctgc gctcgctcta
     4441 ctcggccatc gggaagctga tccacacctt cgagttcctg caccacgggc tggcctgatc
     4501 ctgggattca tcggcgtcaa gatgctgatc tcgcactggt acaagatgcc ggcgggggta
     4561 acgctggtcg tggtcatcac cgtgctcacg tcgacggtgg tgtcgtcgat ctggctgcgc
     4621 aaaatcaagg agtcgcggcg gaaagcctct tgagcttcaa gaacaactcg gccagcgcgc
     4681 cgtcgatgtt ggcgttggcg aggaagacct gcgcgcgcgt ctccaggatc gattcgatgg
     4741 cgcgcgggag cttggcactg ccccacacgg cgcccacgcg ctcgatgtcc ttcgcgacgt
     4801 ccgcgttgac gtagagcgac gcgtcccccg tcatcgcgtc gcggaaaatc accgccatct
     4861 cgtccagcac gcgcgcgacg ctctcgcgat tggccccgcg cgccccgatc agcgcctgcg
     4921 agagcgccca cgactccttc tccttggcca gtccggcgac cagcccgagg gccgcgtcgc
     4981 gctccgcgaa atccgccgag tcgagcaacg cgcgcgcgcg cacgatcgac ccttgcgcga
     5041 gggcggccgc gcgcttggcc acttgggcgt cggcgttgta gtaggagatc aggatcttct
     5101 ccagcaccgc gcgcttgacc gggaagaagc gcacacgctg gcagcgcgag cgaatcgtgt
     5161 agagcaaccc ctccgcattc ggggtaacaa agacgatggc ggcgtgcgcg gggggttctt
     5221 cgaccagttt caagagcgcg ttctgcgctt cttcgcgcat ggcatgcgcg tcgacgaaaa
     5281 tgaagacgtg gtagcgcgcg tcgaagggct tggtgaaagc cctctgaatg accgcgcgcg
     5341 cgcgcgccac gcggatgctg gtcttcttgg tgaaggtcgg ggccgcgaag atgtcggcgc
     5401 gcttttcttc caggatcttc gcgatgtcgc cgacgtcgcc ttcgtcgtcc tcgttggagc
     5461 cctgcgtggg ggtcgggtag acgagatgaa tgccggggtg ctccaagatc gacgcgtgca
     5521 ggcacgacgc gcacctgggt ccgtcgaggc ggcacggttc gtccttctcg cactgcagcg
     5581 cgcgcgcgaa ctcgatggcc gtgaactctt tcccggttcc ctcggggcca tcgaacaaga
     5641 gcggcgggat cgcaccgcgc ttcaacgaac ggagatacgc cgatgcctgc ggctgctcga
     5701 ggtcgagcgc gggcaggggg cgcggttctt gggaggcacg cggcggcatg ccgcgagtat
     5761 agtcgttctc cggcgtcttt gtcaggcgac tcgctatgac ccctgcttct tgcccagcca
     5821 ctccgccggg ttcatcgcga ggcggccccg tcgcacttcg aagtggcaga tgtagccctc
     5881 cagcgatccc gtgtctccca cctccgcgat cgcctgcccg cgtcccactt tctgtccttg
     5941 cgccaccatg gtggtggcga cgtgcgcgta gagggtgtag aagccgccgc cgtggttcaa
     6001 gatgacgcac ttgccgaagg cgtcaatcca gtccacgaac tccaccacgc cggtagcgac
     6061 cgcgacgatg ggcgcgccgg cgggcgcctg gatgtcgacg ccattgttca tggtcacggt
     6121 tccgtacttg ggatgggtgt gcttgccgaa gccgcggatg atcttccccg ccaccggcca
     6181 caataggcgc cccttcatcg cggcgaactc gcccgtgggc ggcaggtcct tgccggccgt
     6241 gccgcgcgcg atgatgtcgt cgatcagcgc ctgcaactcg gcttgcgccg cttcgaggtc
     6301 ttcgatcgcc ttggcgtgct tggatttctc gctgcgaatg ccggccagta cggtctgtcg
     6361 ttgcttcttc gagtgctgga gctgtgccgt ctcgcgctcg cgcgcttcgc gcagctccca
     6421 cacttcctgc agcgactcgg ccaagcgcgc cgtctcgatc tcgagagact ttttgctctc
     6481 cttgaactcg ccgatcaagt tggcgtcctg cgacgcgatc agcttcatga actggtagcg
     6541 cgtgatggcg tcgtcgatgc tctgggcgcc gaggaagacc tcccacgtgt actggggatc
     6601 gcgcttgtac atttgccgca agcgcttgcc cagcgcttct tcttgctgcg acagcacgat
     6661 ctcgcgcccg ccgatcatca ccttgagatc gccgatgcgg ttgtcgatgg tgctctcttg
     6721 ctcggtgagc ttcttgagat agcgcttcga gaggtcgatg tccttgtcga cggcggagag
     6781 tttcttcaag acttgcttct cctgcgagcc gagcttctgc gacttcgcgc ggtgctcctc
     6841 gatctcgcgt cgaatgcgat cgagctccgc ctgcttctcg tcgacgtcgt cggtctgcgc
     6901 ccacgccaca accgtggtca acgcggcggc caacgccgcc gttagcaata cggcgcgaag
     6961 cgggcgcatc attgcatccg taggtatttt cggagcgcca tgaagctccc gagcgcgccg
     7021 atcagaacgc acgtcaagaa gaagaccagg gacttctcga ccgagaagaa gttgatatcg
     7081 gggatggcac tgcggctcac ctcggtgacg acgcgcagca gtagaagcga caggaccgcc
     7141 gccacgccgc cctggaacgc gccctcgatg atgaacggcg ccacgatgaa cgggttggtg
     7201 gcacccacga gcttcaggat atcgatggtc ttgcgccggc tgatcacggt caggcgcacc
     7261 gcgttcgcga tgatgaagat ggccgacatg atgacgatga agcccaccac gatgtcgatc
     7321 gtataaatgc ccttcacgat cgagtcgaac ttgtcgagga actccttgcc gtagcgaact
     7381 tcctcgacgc cgcgcatgtg cccgaagcgc tcggcgagcc cgaccatggc gtcgcgcgac
     7441 ttgtgctccg ccttgggctc gatccagaac gagttgggca gcggattggc gcgcagcgcg
     7501 gacaaaagct ccgcgtcgtc gcccagctgt cccttgaagt ccacgagcgc ttcttccttg
     7561 gacacgaact ggacgctcga gacttcgggg gtggccaaga tcgagcggta caactcctcg
     7621 atgtgcgcct gccccacgtt gtcgtcgagg tagacgtaga tcttcatttc ctcttgggtc
     7681 tcgctcacga gcttgaggag attgtcggtg gccagcagga agacggccag catcaggagc
     7741 gagaggctca tgatgacgac ggagatcgac gtgaggcctt tgtggcgccg cacggtgcga
     7801 agcgcttcgt cgacgatata gagaatgcgt cgcatcatgg tcgatttact ccgtcttggt
     7861 cggcgccagc gaggtcgtgc gctggcgctt gatggcgtcg ttgacgcggc gcgcgcggtc
     7921 gcgctccatg cgcagcgcgt ccagacggtc gctcaagttg ccgaccaggc ccgtcgtctt
     7981 gtcttcgtac accttgccgt cgcgcaggtg aatgatgcgc tggccgaagt tctgcacgaa
     8041 cgaatggtcg tgcgtggcca tcaagaccgc ggtgccgccg ttgttgatct tgaacaagag
     8101 gcgcagcacg tcctccgcgg aatccgggtc gaggttgccg gtaggctcgt cggccaaaag
     8161 aatcgcggga tgattgacga gtgcccgcgc gatcgcgacc cgctgctg
//
LOCUS       DASPGK010000098        10882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_727619, whole genome shotgun sequence.
ACCESSION   DASPGK010000098 DASPGK010000000
VERSION     DASPGK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727619"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /gene="tkt"
                     /locus_tag="VEC56_02810"
     CDS             <1..1934
                     /gene="tkt"
                     /locus_tag="VEC56_02810"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXV13113.1"
                     /translation="MGAAPMAFALWDRVLKHNPGDPAWFDRDRFILSAGHGSMLLYAL
                     LHLYGYDLPLDQLVQFRQFDSRTPGHPEYGHTPGVEATTGPLGQGFAIGVGMAMAEAH
                     LAARFNIPGEPPVVDHYTYAIVSDGDLMEGVSNEAASLAGTLKLGKLVYLYDDNRITI
                     DGSTALAFTEDVGARFEALRWHVQAVEDGNDVDAIERAIRAAREEKDRPSLIRVRTHI
                     GFGSPKQDSEKAHGEPLGADAVRASKEKLGWPQTPLFHVPKEVAAHCREASERGSKLE
                     SEWSERVRAYGKKHRESGALFEAARFEKFPKGWDRELPVFPKEKPVATRAASGKAINA
                     IASKIPALFGGSADLTGSNNTRIESSKTFALDGYDQRNVYFGVREHAMGAAVNGMALH
                     GGLIPFAGTFLIFSDYMRPSIRLSALMRVPSTFIFTHDSIGLGEDGPTHQPVEHLMSL
                     RAIPGLTVFRPADANETAAAWACALSLRGPRALVLTRQALPILDVDPEDVRKGVSRGA
                     YVVAEASKRKPDVVLIATGSEVHLALESRTVLEARGVATRVVSMPSWELFLAQAPRYR
                     NSVLTRSARKVAIEAGATLGWREFVGDKGAIVGLDRFGASAPGPVAMEKLGFSVDNVV
                     NTALSVTKRGGSRVSSKNG"
     gene            complement(1931..2443)
                     /locus_tag="VEC56_02815"
     CDS             complement(1931..2443)
                     /locus_tag="VEC56_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13114.1"
                     /translation="MNRLVFLAVALLVAVPSRAEVLHGTATLEFWSSCGLYWGGNAID
                     FESQSTVGYYVLEADLSLSEPVNYCGRLNLWFSNGGHGAQVGGSLEELTTAPEPLGQW
                     VMIMPPVDEACVLKTVGGLYVKFAVRALRMGAREVDIEYYVQTDGTPEFGPVLPVTAT
                     TWGQIKALYR"
     gene            2596..3009
                     /locus_tag="VEC56_02820"
     CDS             2596..3009
                     /locus_tag="VEC56_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HXV13115.1"
                     /translation="MNCPICEVPMIVLELDQVEVDHCRKCGGVWLDPDEMDLLLEGSA
                     GREEVRASLKFGEPGAEKTHRCPICRHKMEKARVRRNAGGEGIQIDRCERGHGTWLDG
                     GELHAIIALSDFPEAHRIHEFLQAVFRDSNRETRA"
     gene            3038..3595
                     /locus_tag="VEC56_02825"
     CDS             3038..3595
                     /locus_tag="VEC56_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXV13116.1"
                     /translation="MAALIFLGVVVLAVMFFVGIYNSLVGLRNRVKNAWSQIDVQLKR
                     RHDLIPNLVETAKGYMTHERETLSAITEARSRAMGAGSVAEKGVAETQLTQALSKFMV
                     VVENYPDLKANQNFLSLQEELTSTENKIAFARQSYTDAVNFYNNKIEMFPSNVVAGMF
                     NFRPETFFEIEDAAQREAPKVSFTR"
     gene            3598..5085
                     /locus_tag="VEC56_02830"
     CDS             3598..5085
                     /locus_tag="VEC56_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXV13117.1"
                     /translation="MFWELIAANRRKSFMLFVGMAALLVTLGYFIGEATMGEGGGTIG
                     IVVATAVWVIMSAASVFGGPDLVLRMSRAKPVTADVHPQLFNIVEEMKIAAGLPAMPK
                     VYIIDEKAPNAFATGLRPEDSSIAVTAGLLTKLNRDELQGVIAHETSHIMNRDVQFLT
                     LASIMLGSIALISQVFLRGMWYSGGSSKRWRSSKKSEGGGGGGALIVVAIVLAILGPI
                     FARILYFSISRQREYLADACAVRLTRYPAGLAGALEKISQSPLHLEAANQVTAPMYTV
                     APLQGAKAASWGSTHPPIQERIAILRGMSEGAGFLSYQHALAKVRGKPSMIMPASALK
                     KDERVAVREAHPDVAREQSEKDKARDVMDLMRAVNGFAFLLCACGLKIKLPPEIEDAS
                     IACPRCGRENEVPRAQVTEFAEVAAAVGAIVGSQAKADAGGPPFAQPAPGGSSGPDAP
                     LEYRRRAHKEWESFSCACGHLLQLSPVFSASQMKCPNCTRITRIV"
     gene            5108..5626
                     /locus_tag="VEC56_02835"
     CDS             5108..5626
                     /locus_tag="VEC56_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13118.1"
                     /translation="MSPEPSAKPTIRERVRHELREYAQVSIYLLICFSAILFYKAAIL
                     HEHGVDAWRFGAAVGKSLILGKFVLIGQAMRIGERRESATLARGILVKSFLFLLLLVA
                     LSLLEELVTSMIHHRPFAASLAERGSVRELVASSVVMLLILIPYVAFMELDDAMGEGK
                     LRRLMFGRRPPE"
     gene            5636..6958
                     /locus_tag="VEC56_02840"
     CDS             5636..6958
                     /locus_tag="VEC56_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13119.1"
                     /translation="MAWTREDQAKAVEVILNKVGGVWFAYYDVDLSRDDPPIPESDAR
                     AVLDAIVLRDGRYLWNGDDAQRTALLPTLTHVFRDHPEIAAEAFRRIFDMNEIPTESG
                     AERGLIGELNRGERRARLDQYLAAVREGKCRDRTILVAEGDSWFQFPGKWVRLFGLRW
                     VHFDAVKDVLDQLMARKKYCVHSLAAGGDWLFEMLRVKDYVEPLSQIEPDAFLLSGGG
                     NDLLGDGRVANMTLHQRRFDPESARHKEILDARLAAVARRRDVVFDREKYAHGVFFLA
                     KEFVSFLNLVLVQYVVFLSDLRRSKLSDMRVVTHGYDFAIPTPKSTARLISIRRLVNK
                     VMGSGKWLWVPLEQKRLSDDDKRAVLYAMITEFNELLVSLAESPRFPRLHHVDCRGIA
                     RSEKDWYDEIHLTSKGYRRAAALFERCIKSALASDEPRQKVFTTADLS"
     gene            6993..7967
                     /locus_tag="VEC56_02845"
     CDS             6993..7967
                     /locus_tag="VEC56_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13120.1"
                     /translation="MHRRISRFVAALALVLTSCAIEPSPPLPPHTFAFAVFGDGPYRA
                     WEVGRFRHMIKDVNRTDLAWFLHVGDIFWYPCSNLNYERALEAMNAIQHPVVYTPGDN
                     EWADCHDRLAGSHDPLERLAHLRKTFFANPGRSVGQHPMPLEPQSEDPAYAEFVENAR
                     WMHGGFVFATVHMVGAENATMPFEGRGAANDEEAARRIRAGIAWMESAFALADSMGLK
                     GVVIAMHGSPGLHYHPRPRPGYVEFLERLEALTKAFGGPVLLIHGDGHQYQADHSLRD
                     RATYEPLKNFTRLETYGSPDIGWVRVVVDTVSGEFTFEPRMMRRWWLW"
     gene            8253..9470
                     /locus_tag="VEC56_02850"
     CDS             8253..9470
                     /locus_tag="VEC56_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HXV13121.1"
                     /translation="MRERITPKYITRLEQVPQLNDAERERLQPVHDAFVFRTNDYYQS
                     LIDWNDPDDPIRRIVMPDTTELEPWGRLDASNEADYTRVPGLEHKYRSTALLLVNDVC
                     GAYCRFCFRKRLFMDDNDEVVRDVSQGIAYIREHREITNVLLTGGDPLLMSTRLLRSV
                     IEPLRAVEHVQIIRIGSKMPAFNPFRILDDPSLCEMLREFSTPAKRIYVMCHFNHPRE
                     LTDEAIEGLARLQEAGVITVNQTPLIAGVNDDPMVLAELFRKCSFVGMPPYYVFQCRP
                     TLGNRGYTVPVERGYQIVEAAKARVSGLAKRARFVMSHETGKIEVIALTSELVVFKYH
                     RAADDADSGRVMVYGRNPDATWFDDYTALVELHAPEPMIALVSGAADERDCCAASMYR
                     SARARSPDIRVVR"
     gene            9666..>10882
                     /gene="ftcD"
                     /locus_tag="VEC56_02855"
     CDS             9666..>10882
                     /gene="ftcD"
                     /locus_tag="VEC56_02855"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXV13122.1"
                     /translation="MKIVECVPNFSEGRDRGVIDAIVAAIAGVEGAHVLDVDPGRDTN
                     RTVVTFVAPLERAMEAAYRGVAKAAELIDMSKHHGEHPRMGATDVCPFVPLGTTTMEE
                     CVALARALGERVGKELGIPVYLYEFAATHDDRRNLASIREGEYEGLPEKLTHAHWKPD
                     FGPAKFNARSGATVIGARKFLIAYNVNLNTRDKKKASDIAFAIREQGRKRRDEHGRFI
                     RDMNGDFVFDAGTLKACKAVGWYIDEYQRAQVSINLTDFDVTPPHVAFEEVARQAAER
                     GLRATGSELVGLVPLAAMRRAGEFYLEKQGASVGVPEPVLVETAIQSLGLRELGGFDP
                     MQKIIEYRVANDAPLASSSLKQFVDLTSTDAPVPGGGSVAALCGSLAASLTAMVANLT
                     FGKKGYESVSSEM"
BASE COUNT         1855 a         3559 c         3596 g         1872 t
ORIGIN      
        1 cgatgggtgc cgcacccatg gccttcgcgc tctgggaccg cgtgctcaag cacaatccgg
       61 gtgatcccgc gtggttcgac cgcgaccgct tcattctctc agccggtcac gggtcgatgc
      121 tgctctacgc gttgttgcac ctctacggct acgacctccc gctcgatcag ttggtgcaat
      181 tccgccagtt cgacagccgc acgcccggcc accccgagta cggccacacg cccggcgtcg
      241 aggcgaccac cggcccgctt ggccagggct tcgcgattgg ggtcgggatg gcgatggccg
      301 aagcgcatct ggccgcgcgc ttcaatattc ccggcgagcc gcccgtcgtc gaccattaca
      361 cgtacgcgat cgtctccgac ggagatctca tggaaggcgt ctcgaacgaa gccgcctcac
      421 tcgcggggac gttgaagctc gggaaactcg tgtatctcta cgacgacaat cgcatcacca
      481 tcgacggctc gaccgcgctc gccttcaccg aagacgtcgg cgcgcgcttc gaggcgctgc
      541 gctggcacgt gcaagcggta gaggatggca acgacgtgga tgcgatcgag cgcgccatcc
      601 gcgccgcgcg cgaggagaag gatcgcccgt cgctcattcg cgtgcgcacg cacatcggct
      661 tcgggagtcc caagcaggac tcggagaagg cgcacggcga gccgctgggc gcggacgcag
      721 tccgcgcctc caaagagaag ctcggttggc cgcaaactcc gttgttccat gttccgaagg
      781 aagtcgcggc gcactgccgc gaagcctcgg agcgcggttc caagctcgaa tcggaatgga
      841 gcgagcgcgt gcgcgcttat gggaagaagc accgcgaaag cggtgcgtta ttcgaagccg
      901 cacgcttcga gaaattcccg aagggctggg atcgcgagct gcccgtattt ccgaaggaga
      961 aaccggtcgc gacgcgcgcc gcctcgggca aggccatcaa cgcgatcgcg tcgaagatcc
     1021 ccgcgctgtt cggcggctcg gcggacctca ccggttccaa caacacgcgt atcgagtcgt
     1081 cgaagacgtt cgcgctggac ggctatgatc aacgcaacgt ctatttcggc gtacgcgagc
     1141 acgcgatggg ggcggccgtc aacggcatgg cgctccacgg cggactgatc ccgttcgccg
     1201 ggacgttctt gatcttttcg gactacatgc gcccgtcgat tcgtctctcc gcgctgatgc
     1261 gggtgccgtc gaccttcatc ttcacgcacg actccatcgg cctgggcgaa gacggcccca
     1321 cccaccagcc cgtcgaacac ctgatgagcc tgcgcgcgat tccggggctg acggtgtttc
     1381 gccccgcgga cgccaacgag accgcggccg cgtgggcgtg cgcgctttcg ctgagaggcc
     1441 cgcgcgcgct ggtcttgacg cgccaggcgc tcccgatcct cgacgtcgac cccgaggacg
     1501 tgcgcaaggg tgtctcgcgc ggggcgtacg tcgtggccga ggcgtccaag cgcaagcccg
     1561 acgtcgtgct gatcgcgacc ggctccgagg tccacctcgc gctcgagtcg cgcaccgtcc
     1621 tggaagcgcg cggtgtggcc acgcgcgtgg tgtcgatgcc gtcctgggag ctgttcctcg
     1681 cccaggcgcc gcgctaccgc aattcggtgc tgacgcgctc ggcgcgcaag gtcgcgatcg
     1741 aagccggtgc caccctgggc tggcgcgagt tcgtcggcga caagggtgcc atcgtcggcc
     1801 tcgaccgctt cggcgcctcg gcgccgggcc cggtcgcgat ggagaagctc ggattcagcg
     1861 tggacaacgt ggtgaacacc gcgctctcgg taaccaagcg cggcgggtcg agggtctctt
     1921 cgaagaacgg ctagcggtag agcgctttga tctggcccca ggtcgttgcc gtaaccggca
     1981 aaacgggccc gaattctggc gtgccgtcgg tttggacgta gtattcgatg tcgacttcgc
     2041 gtgcgcccat tcgaagcgcg cgcaccgcga acttgacgta gagcccgccg actgtcttca
     2101 gcacgcatgc ttcgtcaacg ggcggcatga tcatgaccca ttgtccgagg ggctccggcg
     2161 cggtggtcaa ctcctcgagc gatcctccca cctgtgcgcc gtgcccgccg ttcgagaacc
     2221 atagattcag ccgaccgcaa taattcaccg gttcagacag cgacaggtcg gcctcgagta
     2281 cgtagtaacc caccgtggat tgcgactcga agtcgatcgc atttccaccc cagtacaagc
     2341 cgcaactaga ccagaactca agagtcgctg ttccgtggag tacttcggca cgggagggga
     2401 ccgcgaccaa gagcgcgacg gcgaggaaga ctaggcggtt catacgcacc tccacagacc
     2461 ctggagcata gcacggcaag ggggggtgaa gtgcgttctc gtcctttctt ggcgtcccgt
     2521 ggtaggcttc gcccgtagtc ggtcgaatag cgcgaagcgg cccgggtaac aggtgccgtg
     2581 gacacggcaa ccgcaatgaa ctgtcccatc tgcgaagtcc ccatgatcgt cctcgagctc
     2641 gaccaggtcg aggtggacca ctgccggaaa tgcggcggcg tctggctcga cccggacgag
     2701 atggatcttc tgttggaggg ttccgcgggg cgcgaggagg ttcgcgccag cctgaaattc
     2761 ggggagccgg gcgcggagaa aacgcatcgt tgccccatct gccggcacaa gatggagaag
     2821 gcccgtgtgc gccggaacgc cggaggcgag ggcatccaga tcgaccgttg cgagcgcggc
     2881 cacgggacgt ggctcgacgg cggggaattg cacgcgatca tcgcgctgtc ggatttcccg
     2941 gaagcgcatc ggattcacga gtttctgcag gcggtctttc gcgatagcaa ccgcgagacg
     3001 cgcgcatagc tcgagttagc gaaggaagga gtcagcaatg gctgcattga ttttcttagg
     3061 ggtcgtcgtc ttggcggtca tgttcttcgt cgggatctac aactcgctgg tcgggttgcg
     3121 caaccgcgtc aagaacgcgt ggtcgcagat cgacgtgcag ttgaagcgcc gccacgacct
     3181 cattcccaac ctggtcgaga ccgcgaaggg ctacatgacg cacgagcgcg agacgctctc
     3241 cgcaatcacg gaggcgcgca gccgcgccat gggcgcgggc tcggtggccg agaagggtgt
     3301 ggcggagacg cagctcacgc aggctttgtc gaagttcatg gtcgtggtcg agaactaccc
     3361 cgacttgaag gcgaaccaga acttcctctc gctccaggaa gagctgacct cgaccgagaa
     3421 caagatcgcg ttcgcgcgcc agagctacac cgacgcggtc aacttctata acaacaagat
     3481 cgagatgttc ccgtcgaacg tggtcgcggg catgttcaac ttccgcccgg agacgttctt
     3541 cgagatcgaa gacgccgcgc agcgcgaggc gcccaaggtg tcgttcacgc gctagacatg
     3601 ttctgggaac tgatcgccgc caatcgtcga aagtcgttca tgctgttcgt cggcatggcc
     3661 gcgctcctgg tcacgctcgg ctacttcatc ggcgaagcga ccatgggcga gggcggcggg
     3721 acgatcggca tcgtcgtggc gaccgcggtg tgggtgatca tgtcggccgc gagcgtgttc
     3781 ggcggccccg acctggtgct gcgcatgagt cgcgccaagc ccgtcacggc cgacgtccat
     3841 ccccagttgt tcaacatcgt cgaggagatg aagatcgcgg ccgggctgcc tgccatgccc
     3901 aaggtctaca tcatcgacga gaaggcgccc aacgcgttcg ccaccgggct tcgccccgag
     3961 gacagctcga tcgcggtcac cgcgggtctg ctcaccaagc tcaaccggga cgagctgcaa
     4021 ggggtcatcg cgcacgagac gtcgcacatc atgaaccgcg acgtgcagtt cttgaccctc
     4081 gcgtcgatca tgctcgggag catcgcgctg atctcgcaag tgttcctgcg cggcatgtgg
     4141 tacagcggcg gctcgtccaa gcgctggcgt tcgtcgaaga agagcgaagg cggtggcggg
     4201 ggtggcgcgc tgatcgtggt cgccatcgtg ctcgccattc tggggccgat cttcgcccgc
     4261 attctctatt tttcgatctc gcgccagcgc gagtacctgg ccgacgcgtg cgccgtgcgc
     4321 ctgacgcgtt atccggccgg actcgcgggc gcgctcgaga agatctcgca gtcgcctctc
     4381 catctcgaag cggccaacca ggtgacggca cccatgtaca ccgtcgcccc gttgcagggt
     4441 gccaaggcgg cgagctgggg ctcgacgcac ccgcccatcc aggaacgcat cgcgattttg
     4501 cgcggtatga gcgagggcgc ggggttcttg agctaccagc acgcgctcgc gaaggtgcgc
     4561 ggcaagccgt ccatgatcat gccggcctcg gcgctcaaga aggacgagcg cgtcgcggtg
     4621 cgcgaggcgc accccgacgt ggcgcgcgaa cagtccgaga aggataaggc gcgcgacgtg
     4681 atggacctca tgcgcgcggt caacggcttc gcgtttctcc tgtgcgcgtg cggcctgaag
     4741 atcaagttgc cgccggagat cgaggacgcg agtatcgcgt gcccgcgctg cggtcgcgaa
     4801 aacgaggtcc cgcgcgcgca ggtgaccgaa ttcgccgaag tggccgccgc cgtgggtgcg
     4861 atcgtggggt cgcaggccaa ggcggacgcg ggcgggcctc ccttcgcgca gccggcgccg
     4921 ggcggatcgt ccggtcccga cgcgccgctc gagtaccgcc gccgcgcgca caaggagtgg
     4981 gagtcgttct cgtgcgcgtg cgggcatctc ctgcagctgt cgccggtgtt ttcggccagc
     5041 cagatgaagt gtccgaactg tacgaggatc acgcggatcg tgtagcctgc tcgcacagga
     5101 ggcgtccatg agtcccgaac cgagtgcgaa accgacgata cgcgagaggg tgcgtcacga
     5161 gctgcgcgaa tacgcgcagg tgtcgatcta cctgctgatc tgcttcagcg cgattctctt
     5221 ctacaaggcc gcgattctcc acgagcacgg cgtcgacgcg tggcgcttcg gggccgcggt
     5281 gggcaagtcg ctcatcttgg ggaagttcgt cctcatcggg caggcgatgc gcatcggcga
     5341 gcggcgcgag agtgcgacgc tggcacgcgg catcctggtg aagtcgttct tgttcctcct
     5401 cctactcgtc gctctctcgc tgctcgaaga gctggtcacg tcgatgatcc accatcgccc
     5461 cttcgcggcg tcgctcgcgg agcggggcag cgtgcgcgag ctggtcgcat ccagcgtcgt
     5521 catgctcttg atcttgattc catacgtcgc gttcatggag ctcgacgacg cgatggggga
     5581 aggcaagctc cggcgcctga tgttcgggcg gcgtccgccg gagtaggttg acaccatggc
     5641 ctggacgagg gaggaccaag ccaaagcggt cgaggtgatc ctcaataagg tcggcggcgt
     5701 ttggttcgcc tactacgacg tcgatctctc gcgcgacgac ccgcccatcc ctgaatccga
     5761 cgcgcgcgcc gtcctcgatg cgattgttct ccgcgacggc cgctacctct ggaacggtga
     5821 cgacgcccag cgcaccgcgc tcctgccgac cttgacccac gtctttcgcg accaccccga
     5881 gatcgcggcg gaggcgtttc gtcgcatctt cgacatgaac gagatcccca cggagtccgg
     5941 cgccgagcgc ggcctgatcg gcgagctgaa ccggggcgag cggcgcgcgc gcctggacca
     6001 gtacctcgcc gcggtgcgcg aggggaagtg ccgcgaccgt accatcctgg tggcggaagg
     6061 cgactcgtgg tttcaattcc ccggcaagtg ggttcggctc ttcggcctgc gctgggtcca
     6121 tttcgacgcc gtcaaggacg tgctcgacca attgatggcg cgcaagaagt actgcgtcca
     6181 cagcctcgcg gccggcggcg actggctctt cgaaatgctg cgcgtgaagg actacgtcga
     6241 accgctctcg cagatcgagc ccgacgcctt cctgttgagc ggcggcggca acgatctctt
     6301 gggcgacggg cgcgtggcca acatgacgct gcaccagcgg cgcttcgacc cggagtcggc
     6361 acggcacaag gagatcctcg atgcgcgtct cgcggcggtc gcgcgccggc gcgacgtcgt
     6421 cttcgaccgc gagaagtacg cgcacggcgt cttcttcttg gcgaaagagt tcgtgagctt
     6481 cctgaacctg gtgctggtgc agtacgtcgt cttcctctcc gacctgcggc gatcgaaact
     6541 gtccgacatg agagtggtga cgcacggcta cgacttcgcg attccgaccc cgaagtctac
     6601 cgcgcgtctg atctccattc gccggctcgt gaacaaggtg atgggatcag gcaagtggct
     6661 gtgggtcccg ctcgagcaga agcgcctctc cgacgacgat aagcgggccg tcctgtacgc
     6721 gatgatcacc gagttcaacg agctcttggt ttcgctggcg gagtcgccgc ggtttccgcg
     6781 cctccaccac gtcgactgcc gcggcatcgc gcgctcggag aaggactggt acgacgagat
     6841 ccatctgacg tcgaaggggt accgccgcgc ggcggcccta ttcgagcggt gcatcaagag
     6901 tgcgcttgct tcggacgaac cccggcagaa ggtgttcacg accgccgact tgtcgtaggc
     6961 ccgaacggcg gcgctcgtgg tatggtgtac cgatgcaccg ccgtatatcg cgcttcgtgg
     7021 cggcactcgc actcgttctc acgtcgtgtg cgatcgagcc gtcgccgccg ctcccgcccc
     7081 acaccttcgc cttcgccgtc ttcggcgacg gaccgtaccg cgcgtgggag gtcgggcgtt
     7141 ttcgccatat gatcaaggac gtcaatcgca ccgacctcgc gtggttcctc cacgtcggcg
     7201 acatcttttg gtacccgtgc tcgaacctca actatgagcg ggcgctcgag gcgatgaacg
     7261 cgatccagca ccccgtcgtc tacacgcccg gcgacaacga gtgggccgac tgtcacgacc
     7321 gcctcgccgg cagtcacgac ccgctcgaac ggctcgcgca cttgcgcaag acgttcttcg
     7381 cgaatccggg acgaagcgtt ggccagcacc ccatgccgct cgaaccgcag agcgaagacc
     7441 ccgcctacgc cgagttcgtg gagaacgcgc gctggatgca cgggggtttc gtattcgcga
     7501 ccgtccacat ggtgggcgcg gagaacgcca ccatgccgtt cgaggggcgc ggggccgcga
     7561 atgacgaaga ggcggcgcgt cgtatccgcg cgggcatcgc atggatggag tcggcgttcg
     7621 cgctcgcgga ctccatgggc ttgaagggcg tcgtgatcgc gatgcacgga agccccggac
     7681 tgcattacca cccgaggccg cgcccgggat acgtcgagtt cctggagcgg ctcgaggcgc
     7741 tgaccaaggc gttcggcggc cccgtgttgt tgatccacgg cgacgggcac cagtatcagg
     7801 ccgatcattc gctgcgcgat cgcgcgacct acgagcccct gaaaaacttc acgcgtctcg
     7861 agacgtacgg atcccccgat atcggatggg tccgcgtggt ggtcgatacg gtctcgggag
     7921 aattcacatt cgagccccgc atgatgcggc gctggtggct atggtgagaa aagggttgca
     7981 cgcctcgccg atctgggcta attttgtcat ggcccttgcg gcggtggccc atgcggccgg
     8041 gatcgcgtgc ccatcaatgg cgcgcacata gtcaatcagt cgagacgctt cataacctta
     8101 cggatgacga cgcatcgtcg gcgtccgatc cgccctgtga accggcacgc gccggcgacc
     8161 ggggcaaaat aatccgagcg cggctctcac acggtgctac cggcccgttt gccgcgtaca
     8221 taatcgatca ccccagtcca cggaggtgag gtatgcggga acgtatcact cccaagtaca
     8281 tcacgcgcct ggaacaggtg ccccagttga acgacgccga gcgcgagcgc ttgcagcccg
     8341 tgcacgacgc cttcgtgttt cgaaccaacg actactacca gtcgttgatc gactggaacg
     8401 accccgacga tcccatccgg cgcatcgtca tgccggatac caccgagctc gagccctggg
     8461 ggcgcctcga cgcgtccaac gaggccgatt acacgcgcgt gcccggtctc gagcacaagt
     8521 accgctccac ggccttgctc ctggtcaacg acgtgtgcgg cgcgtactgc cgcttctgtt
     8581 tccgcaagcg cttgttcatg gacgacaatg acgaggtcgt gcgcgacgtc tcgcagggta
     8641 tcgcgtacat ccgagagcac cgcgagatca ccaacgtgtt gctcacgggc ggcgacccgc
     8701 tgctgatgtc cacgcggctt ctccgttccg tgatcgagcc cctgcgcgcc gtcgagcacg
     8761 tgcaaatcat tcgcatcggg tcgaagatgc ccgcgttcaa cccgtttcgc atcctcgacg
     8821 acccctcgtt gtgcgagatg ctgcgcgagt tcagcacgcc cgcgaagcgc atctacgtga
     8881 tgtgccactt caaccacccg cgtgagctga ccgacgaggc gatcgaaggc ctcgcgcggc
     8941 tgcaggaggc gggggtcatt acggtcaacc aaaccccgct catcgcgggc gtcaacgacg
     9001 accccatggt gctggcggag ctgttccgca agtgctcgtt cgtgggcatg ccgccgtact
     9061 acgtgttcca gtgccgcccc acgctgggca accgcggcta cacggttccg gtggagcgcg
     9121 gctatcaaat cgtcgaggcg gccaaggcgc gtgtgtcggg gctggccaag cgcgcgcgct
     9181 tcgtcatgtc gcacgagacc ggtaagatcg aagtgatcgc gctcacctcg gagctagtgg
     9241 tgttcaagta ccaccgcgcg gccgacgacg cggattcggg tcgcgtgatg gtgtacgggc
     9301 gcaacccgga tgccacctgg ttcgacgact acaccgcgct ggtggagttg cacgcgccgg
     9361 agccaatgat agctctcgtt tcgggcgcag ccgacgagcg cgactgctgc gcggcttcga
     9421 tgtatcggag cgcgcgtgca cgaagtcccg atatccgcgt ggttcgctag ggtcgtagtc
     9481 agcgatcgat agcagcaccc tccctcggcc ctgcgggccg cgcggtatat aaccagccgg
     9541 cggaagcgcc gagcgcgttc agcttgtcgc ggggtgaatt ggcgccccgc ctgcaacctc
     9601 ctattacgcg cccgcgctcc gccggccctt cgcccgcggg ccgcgatcgt gtatgatcgc
     9661 ggcccatgaa gatcgtcgaa tgcgtcccca attttagcga gggccgcgac cgcggcgtta
     9721 tcgacgcgat cgtggccgcc atcgcgggcg tcgaaggcgc ccacgtcctc gacgtcgacc
     9781 ccggccgcga caccaaccgc accgtcgtca ccttcgtcgc cccgctcgag cgcgcgatgg
     9841 aagccgcgta tcgcggcgtc gccaaggccg ccgagctcat cgacatgtcc aagcaccacg
     9901 gcgagcaccc gcgcatgggg gcgaccgacg tgtgcccgtt cgtgcccctc ggtacgacga
     9961 ccatggagga gtgcgtcgcc ctcgcgcgcg cgctcggcga gcgggtgggc aaggagctcg
    10021 gcattccggt ttacttgtac gagttcgccg cgacccacga cgaccggcgc aacctcgcca
    10081 gcatccgcga gggcgagtac gagggtctgc cggagaaact cacgcacgcg cactggaagc
    10141 ccgacttcgg ccccgccaag ttcaacgcgc gctccggcgc taccgtgatc ggcgcgcgta
    10201 agttcctgat cgcctacaac gtcaatctca acacgcgcga caagaagaag gcgagcgaca
    10261 tcgcgttcgc gatccgcgaa caggggcgca agcgccgcga cgagcacggg cgcttcattc
    10321 gcgacatgaa cggcgatttc gtgttcgacg cgggaacgtt gaaagcgtgc aaggcggtcg
    10381 ggtggtatat cgacgagtac cagcgcgcgc aggtctccat caatctgacc gacttcgacg
    10441 tgaccccgcc gcacgtcgcc ttcgaggaag tggcacgcca ggccgccgag cgcgggttgc
    10501 gcgcgaccgg gagcgagctg gtgggattgg tgccgctcgc ggccatgcgc cgcgcggggg
    10561 agttctactt ggagaagcag ggcgcgtcgg tgggcgtgcc cgaaccggtg ctcgtggaga
    10621 ccgcgatcca gtcgctgggg ctgcgcgagc tgggaggatt cgatcccatg cagaagatca
    10681 tcgagtatcg cgtggcgaac gatgcgccgc tggcgtcgtc gtcgctcaag cagttcgtcg
    10741 acctgacctc cacggacgcc ccggtcccgg ggggcggctc ggtggcggcc ctgtgcgggt
    10801 cgctggcggc gtcgctcacg gccatggtgg ccaacttgac cttcgggaaa aaaggctacg
    10861 agtccgtctc tagcgagatg ag
//
LOCUS       DASPGK010000099        11614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_612185, whole genome shotgun sequence.
ACCESSION   DASPGK010000099 DASPGK010000000
VERSION     DASPGK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612185"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VEC56_02860"
     CDS             <1..232
                     /locus_tag="VEC56_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13123.1"
                     /translation="TSPSFVAPGASAALGGPGPGVSGGGGGGGGGGTDEGDADGLSGL
                     KGRPPLNGSANRDVPNSFFLELWWKFMILWAR"
     gene            297..3341
                     /locus_tag="VEC56_02865"
     CDS             297..3341
                     /locus_tag="VEC56_02865"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV13124.1"
                     /translation="MSDARDEREWLEKGQREQSRGSVVSPGEEIGEMHFATENFSTAL
                     EYFCAALEQPGLVEADQFRLLLRVSDCHRKRGDYAAAKENLDRARALSTLSGEDLGRI
                     EYREGYLLLWQGAYDAALKTGFGAYRRLKRSQSHGEVAYIQLLIANSYQRLGLAAEAE
                     EFFMDALSSYRRAEDRVGIAYVYNSLGLLHKNACRWSRSLASLSKSFDLAKTLGLSQH
                     LIVVQLNLGVVYSKLRRFEDAISSFDAAASGAERFGDQASLTRALLMLGRTHVARGSL
                     GRGEKALLRAQAMADELGYERESALADEYLGELMMARNDLRCAHANLSAALEKGRRLA
                     PQGDIVAESLRRFADVQYRLGEVEGALASIDEGLRIATTCGELYEVGYFHRTRALALA
                     RTGNLPGALESMTTSVASFEEYGNSYEKALSEQLLARLCVRARSEAPLLRAKSALADS
                     VMALGKIDEPRALIVSHVLLSNVERRLGNVDDALLAVYEADRIAADEECAKFRKALAA
                     LRRRIEDQMSNATRRVLDQFSVLGEIQSGARSRERLSKGLESTLRLIIEKLDAASGFI
                     AIPAPNGRGLQVIAREGLAPKEAQAIESWYSKRGASARGPVVVTDFDKDPSLADLRGR
                     LANAGPLLIQDLGFDREGLGIMCIVKSADSARAPIGQDDLHFVAAYSNLISLSVYELA
                     RSERKGRAARPRPGASKGFESIVTDNKQMIHLLNLAERVAHSDATVLLQGETGTGKGL
                     IAYAVHLLSERRDRAFVHVNCAALPEQLLESELFGHVRGAFTNAYFDKDGLLMEANGG
                     TIFLDEIGKTSMAMQGKLLQFLDSGKVRRVGANEFTNVNVRVICASKSNLLKSVEEGR
                     FLEDFFYRINDFPLTVPPLRERPEDIPLLIHHYVGKISLEFGKAIDGTTPEFMGKLVE
                     YHWPGNVRELEKVIKRAIILADDGDALAVAHLAPEVLRGMPEPDAASKNEETLRERIE
                     QIEQAMILEAMRRHAGNKSQVAVHLGISYPNLLSKIKRYNIQ"
     gene            4280..5011
                     /locus_tag="VEC56_02870"
     CDS             4280..5011
                     /locus_tag="VEC56_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13125.1"
                     /translation="MVDAPPFPPDGVFSVTGDGVVSLFWNPNQESDLAGYAIYRGNQA
                     PEGPYFFLADVNASTTNYDDFDVVNEEFWYYAVTAFDAAGNESELSAELVFDTPRPEG
                     FGLVLVELGQDPSRAGYDFSSLSNSSQSASLGTTDIYFESAGGVEYVVAADPLVDIQD
                     YGLIELVAVDWAPLVGWAPSKRAEAIAGHSYIVRIVDGQGDFNMAKFEVRAVTATNLT
                     VDWAYQGVDNNPELSIAPGVGGAMK"
     gene            5008..6180
                     /locus_tag="VEC56_02875"
     CDS             5008..6180
                     /locus_tag="VEC56_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13126.1"
                     /translation="MNSSIRHSIALGLACAAVAALAAAAEAGHGRYAVSAQANVRVWI
                     EGGDVFDNYANVAIWLRPERDCYTTLFMVDTDGYVHVLYPSAPYHDTWLYGGRSYCYR
                     ASDLGLDRLDGAGIAYVYAVGSPVPFDYSAYGAGVFVGGFGFRVYGDPFVACRDFYMT
                     LLPGSCRWDYVGVSYARFYVRHWARYPSYLCYGGPGVHVYVGDGCRACGDVYVSYRNS
                     CSRPWDAFQPVPRFKQVHSGGRIERDDDWNDGRNHARTSRVERVQHESFKSKVTVRSS
                     QREYTAKQFTKPAVRSHEYKAVSKPSPARVETRSTTVTRAKSPVKSHDRARVVSTSSA
                     RERASVDKADVAAMTKQSSRAVRPSRSSDTGKRAVTGKSVRTESTREKGTKKKQAR"
     gene            6490..9738
                     /locus_tag="VEC56_02880"
     CDS             6490..9738
                     /locus_tag="VEC56_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV13127.1"
                     /translation="MVALATLVGAMGARAAQFAPPYRGADARLTTHATPAHVSAPASP
                     NRYQRARIEAMSRATAAVTDDTLRVLALQVQFADSLMGGQPGSNRSAVRDSTWFANEL
                     IHTEQYFRGASRNRFTFEWTLEDSLYSLPRGMGYYGADSREEERVVELAQTLIDLADA
                     NVDFSLYDHVFIIHAGAGQETDINADSPNQIWSSFYDRGDIREIQDDDASLGLPTDDL
                     LGGDPFFVDNFSIVPSHASQDFATVGTLGIWTFEIGSRIGLLPLFDSTPNGAPDSQGV
                     GNFCLMAYGIFNVNGFVPAFPCAFNRILAGWLDPVVVDPDDTPAAIRLADVNTGADAD
                     TLCVKVPITDSEYYLVVNRVHDENFDSLFTFADADSDLVPDNTESLDGAEFDFFLTDL
                     TNPFERRFDPRYGFNVLFRYTGSGVYVWHVDERVVIDAARRGYLPDDYAARKGVDLEE
                     ADGVQDMDRPGSAAFALGSHFDSYRTGDGNNPVFGPDTDPASGTNAGAETGITIETLT
                     GPAFRMRVSIGRLVAYNDTRARWSASARNQPATVVNLDGAGDPEIVTLGDNAGVFVLD
                     ESGREVVDPDANPSTIAPFIAVPNVTWIGPPAFANLDGAPDIEIVAGARNGDVYVWKA
                     DGTPLAGAPFAGLSGAAAPVIVDVGDAGLPEILVASGGTGTQLRFIDATGAPVDPPAA
                     AALWPAPFDAQILAPLAIAGVSGPSVMGVVACGVDTTTSRVVVQWTPLDAARAPWEQA
                     IAIPSGWDAASFVPTAPAVGDVDHDGDDEIVIATPDGSVVVFDMSSTTVTRESGVLRA
                     RYPSAPALGDVDDDGTLEIAIWDAEYMYLLKSNARPMLEWPRAIRDESAGAAPTIRPH
                     RELESPLLADIDGGTVDVVFPLDDGTLAAFRADGRPVASFPRPGPAEAGATPTLTDLA
                     PGSGSLVVLGAYGTLDGVDTVVDTLAHDPSSSLSIQSLSASVTSPYWIMARADLARTG
                     RVVESVPLATSTSTFDEGSFIIYPNPVMESTVHARITTNARARVRLSIHTLEGQEATV
                     REFDVNPNGLVDTPFDEAIDVSGLKSGIYFMRLEIDGPGGSGAVYKTFAIRR"
     gene            9767..10675
                     /locus_tag="VEC56_02885"
     CDS             9767..10675
                     /locus_tag="VEC56_02885"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HXV13128.1"
                     /translation="MRRQLVLATLLVAAFVETAHAGDPGDAGALFLRVGFGARASAMG
                     EAFTAVAEDATSIYWNPGAMAAVLGTKVTLAHTEYFATMRLEQASVTHETDWGTIGFM
                     FTGLYMDEMDRYEDTPSANPLGTFGAYDVAVSAAYARYLVPNLAAGISIKPVYERIDE
                     LSASGIAFDIGLYHVARIQGVKLAAVATNVGPPMKFDQEEFALPRSIKVGGSYEREIA
                     ALDGRILVTLDGVFPNDGEVREHVGAEYSYRGMLALRAGYKVNYDAQDATFGAGFTWH
                     EITVDYAFLPSEKLGDNHRVGLGFSF"
     gene            complement(10665..11423)
                     /locus_tag="VEC56_02890"
     CDS             complement(10665..11423)
                     /locus_tag="VEC56_02890"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HXV13129.1"
                     /translation="MTPANLFEPIAAADVRIVADTHFRDRTLPGESERRDRFIRFLDG
                     TPKGTFLVLLGDIFDFYFEYRSVVPRRFLDVFAAISRATARGVETRFLGGNHDYWVGD
                     FFTRELGVRVHRKELCVEAQGRKLVLAHGDLVMPRDYGYKVLKNVIRNPVVIEVSRWI
                     HPDILDAIAGGVAHGSRVLFHIAQEKRARAVAARAWDHFFSRGNDGFVMGHVHFPMHE
                     TREGKEFLIVGDWIEHYTFVRMTDAHLRIETFKS"
BASE COUNT         1988 a         3727 c         3817 g         2082 t
ORIGIN      
        1 gacctccccg agcttcgtcg ctccgggggc aagtgcggct ttgggcggtc ctggacctgg
       61 ggtttcgggc ggtggcggcg gtggcggcgg cggtggtacc gacgagggcg acgcggacgg
      121 gctttccgga ctgaagggac gtcctccgct caacggctcg gcgaatcgcg acgttccgaa
      181 ttcgttcttc ttggagttgt ggtggaagtt catgattctg tgggcgagat aacggtgtgc
      241 gccccgaagc gggggcgcgc gttcgtttca taaagaccac ttagctcgag gaatctatgt
      301 ccgacgcacg ggacgagaga gagtggctgg agaagggtca gcgcgagcaa tcgcgcggat
      361 cggtggtttc gccgggtgaa gaaatcggcg agatgcactt tgctacggag aacttctcca
      421 cggcactgga atacttctgc gcagcgcttg agcaacccgg actggtcgaa gcggaccagt
      481 tccggttgct gctgcgggtc agcgattgcc accgcaagcg tggggactac gcggcggcga
      541 aagagaatct cgatcgcgcg cgagcgcttt cgaccctttc gggcgaggat ctcggtcgca
      601 tcgagtaccg agagggttat ctgctcctgt ggcagggcgc gtatgacgcc gccctgaaga
      661 ctgggttcgg cgcctaccgt cggctgaagc gatcgcagtc gcacggcgag gtcgcctaca
      721 tccagctcct gatcgcgaac tcctaccagc gtctcggcct cgccgccgaa gcggaggagt
      781 tcttcatgga cgcgctgtcg tcgtaccgcc gggccgaaga ccgcgtcggc atcgcgtacg
      841 tctacaacag cctcggtctc ctgcacaaga atgcctgccg ctggtcgcgc tcgctcgcgt
      901 cgctgtcgaa gtcgttcgac ctcgccaaga cgctcgggct ttcgcagcac ctgatcgtcg
      961 tccagctcaa cttgggcgtc gtgtactcga agcttcgtcg ctttgaagat gcgatcagct
     1021 ccttcgacgc ggcggcgtcg ggtgccgagc gcttcggcga ccaggccagc ctgacccgcg
     1081 cgttgttgat gctgggtcgc acccacgtcg cgcgcggttc gctgggtcgc ggcgagaagg
     1141 cgctcctgcg cgcgcaggcc atggccgacg agctcggata cgagcgcgaa tccgccctcg
     1201 ccgacgagta cctcggcgag ttgatgatgg cccgcaacga tctgcgctgc gcgcacgcca
     1261 acctatccgc ggccctcgag aagggccgtc gcctggcgcc gcagggcgac atcgtggccg
     1321 agagcctgcg ccgcttcgcc gacgtgcagt accggctggg tgaggtcgaa ggcgcgctcg
     1381 cttcgatcga cgagggactt cgcatcgcga ccacgtgcgg cgagctctac gaagtgggtt
     1441 acttccatcg cacccgcgcg ctggccctgg cccgcaccgg gaaccttccg ggcgcgctgg
     1501 aatcgatgac caccagcgtc gcgtccttcg aggaatacgg caattcgtac gagaaagcgc
     1561 tctccgagca gttgctcgct cgtctgtgcg tgcgcgcccg ttcggaagcc ccgctgttga
     1621 gggccaaatc cgcgctggcc gacagtgtca tggcgctggg caaaatcgac gaaccacgcg
     1681 cgctcatcgt gtcgcacgtg ctgttgtcca acgtcgagcg gcgactgggt aacgtcgacg
     1741 acgcattgtt ggccgtctac gaggcggacc gcatcgcggc cgacgaagag tgcgccaagt
     1801 tccgcaaggc gctggcggcg ctgcggcgcc gtatcgagga ccagatgtcg aacgcgacgc
     1861 ggcgcgttct ggatcaattc tcggttctgg gcgagatcca gagcggcgcg cgctcgcgcg
     1921 agcgcctctc gaagggcctc gaatcgacat tgcgcttgat catcgagaag cttgacgcgg
     1981 cctcggggtt catcgcgatt ccggcgccca atgggcgcgg gctgcaggtg atcgcgcgcg
     2041 aagggcttgc ccccaaggaa gcacaggcga tcgagtcgtg gtattcgaag cggggcgcgt
     2101 cggcgcgcgg gccggtcgtg gtcaccgact tcgacaagga tccgtcgctg gcggatctgc
     2161 gcggccgatt ggccaatgcg gggccgctgc tgatccagga cctcggcttc gaccgcgaag
     2221 gcctgggcat catgtgcatc gtcaagtccg ccgactcggc gcgcgcaccc atcggccagg
     2281 acgacctcca cttcgtcgcg gcgtattcca atttgatatc cctgtcggtc tacgagctcg
     2341 ctcgttccga gcgcaagggt cgcgccgcgc gtccgcgccc gggtgcgagc aagggcttcg
     2401 agagcatcgt caccgacaac aagcagatga tccatctgct gaacttggcc gagcgcgtcg
     2461 cgcattcgga cgcgaccgta ctcctgcaag gcgagaccgg caccggcaag gggctgatcg
     2521 cctacgcggt gcacctactg agcgagcgcc gcgatcgtgc cttcgtccac gtcaactgcg
     2581 ccgccctccc cgagcagttg ctcgagtccg agctgttcgg ccacgtgcgc ggtgccttca
     2641 ccaacgcgta cttcgataag gacggcttgc tgatggaggc caacgggggg accatcttcc
     2701 tcgacgagat cgggaagacc tcgatggcga tgcagggcaa gctcctgcag ttcctcgatt
     2761 ccggcaaggt gcgcagagtc ggcgccaacg agttcacgaa cgtaaacgtg cgcgtgatct
     2821 gtgcgtcgaa gtcgaaccta ttgaagtcgg tcgaagaggg tcgcttcctg gaggacttct
     2881 tctaccgcat caacgatttc ccgctgacgg tgccgccgct gcgcgagcgc cccgaggaca
     2941 ttccgctact catccatcac tacgtcggca agatttcgct cgagttcggc aaggccatcg
     3001 atgggacgac gcccgagttc atgggcaagc tggtggagta tcactggccc gggaacgttc
     3061 gcgagctcga gaaagtcatc aagcgcgcta tcatcctggc cgacgatggt gacgcgctcg
     3121 cggtggcgca tctggccccc gaggtgctgc gggggatgcc cgagcccgat gccgcgagca
     3181 agaacgaaga gacgcttcgc gagcggatcg agcagatcga acaggccatg atcctcgagg
     3241 ccatgcgccg gcacgccggt aacaagtccc aggtcgctgt ccacctgggc atcagctatc
     3301 ccaacctcct ctcgaagatc aagcgctaca atatccagta gacccgacga ttttcggccc
     3361 aatcgctggc tgtcttgccg gctacccacg ccttgtaaaa acttttaata acgacttcat
     3421 attgcttcaa cttgttccct gtcagtccgt tagcagagta tcccaggctc gggcatacaa
     3481 ccgttcttaa tagccttccg tttgctttcc acacgccgat cgcgctcgac tgtggattcg
     3541 cgtccgtaac ggaatagcaa acaaagggat agcgcttcgg ccgggcgtcg cgccggcccg
     3601 ccattccgtt ggcatacggg ttgcggtacg cccctatcgg aagtcatccc gtgcgtaaat
     3661 gaacttccat ctggtggggg aagggttctg ggggatcgca agttccccca gagccctgaa
     3721 ctaaagattc aggactggag acagaggaga aacactctcg acccaaagag tcgggagccg
     3781 aaaggcgaac cggattaagc tgacgcccta gattcgttcc taagcaagct gctggctttg
     3841 cggtgtaagt taatttcgag aggtggggtc tcgaaaattg acaacaaggg acggatcggg
     3901 gcgtcagtgt ttcctcccgt cttcgcgtca tttcccagcc aactaaaccc cgggcgccca
     3961 atctcgaaag cgtcctcgtt ttaggtcagc gctgtaactc caacaccacg attctatctc
     4021 ccgccgcgac gcccggcgtc gcattccgag cgccgggctg tctcctaacc gatttcatgg
     4081 caggcggttg accgccctcg gcaatatctg ccagggcgtg gcacgtggct tgcgctaggg
     4141 gatcggcatg atgacgcacc aactgcgtac gcgaaaccca atcgaccgcg cgactcggct
     4201 gtcgggtgcc gtctcgtcgg tcgccgcagt aatgctcatg ctccctttcg ttgctggttg
     4261 cgatgatgaa gaccccgtcg tggtggatgc tccaccgttt ccgcccgacg gggtcttctc
     4321 agttacggga gacggcgtcg tcagcctttt ttggaatccg aatcaggaga gcgatttggc
     4381 cggctacgcg atctatcgcg gcaatcaagc gccggaggga ccgtacttct ttctcgcgga
     4441 cgtcaacgcg agcaccacga attacgacga cttcgacgtc gtcaatgaag agttctggta
     4501 ctacgccgtg accgcattcg acgcggccgg caatgagagc gaattgagcg cggagttggt
     4561 gttcgacact ccgcgcccgg agggattcgg tttggtgctg gtcgaactgg ggcaagaccc
     4621 gtcgcgtgcg ggctacgact tttcgagtct atccaatagc tcgcaatcgg cgtctttggg
     4681 caccacggat atctacttcg agtctgccgg cggcgtggag tacgtcgtcg ccgcggaccc
     4741 gttagtagat atccaggatt acggcctgat cgagttggtg gcggtggatt gggcacccct
     4801 cgtggggtgg gctccgtcga agcgcgcgga ggcgatcgca ggccatagct atatcgttcg
     4861 tatcgtcgac ggccaaggtg acttcaacat ggcgaaattc gaggttcggg ccgtcacggc
     4921 gacgaatctc acggttgact gggcgtacca aggggtagac aacaaccctg agctttcgat
     4981 cgcccctggt gtaggaggtg ccatgaaatg aactcatcaa ttcggcatag catagctctt
     5041 ggcctcgcgt gcgcggcagt ggccgcgctg gccgctgcgg ccgaggccgg tcacgggcgc
     5101 tacgcggtct ccgcgcaggc gaatgtgcgg gtgtggatcg aaggcgggga cgttttcgac
     5161 aattacgcga acgtcgccat ctggctgcgg ccggagcgcg actgctacac cacgctcttc
     5221 atggtcgata ccgacggtta cgtgcacgtg ctctacccga gcgcgccgta tcacgacacc
     5281 tggctctacg gcgggcgctc gtactgctat cgggcgagcg atctcggtct tgaccggctc
     5341 gacggcgccg gtatcgccta cgtgtacgcg gttggcagcc cggtgccctt cgattattcg
     5401 gcctacggcg ccggcgtgtt cgtcggaggc ttcggattcc gcgtctacgg cgatcccttc
     5461 gtcgcgtgcc gcgacttcta catgacgctg ctccccggct cgtgccgctg ggactacgtc
     5521 ggcgtgtcct acgcgcgctt ctacgtgcgc cactgggcgc gctatccgtc gtacctttgc
     5581 tacggcgggc cgggcgtcca cgtctacgtg ggcgacggat gccgcgcgtg cggcgacgtg
     5641 tacgtctcgt atcgcaacag ctgctcgcgc ccgtgggacg cgttccagcc ggtgccgagg
     5701 ttcaaacaag tccattcggg cgggcgcatc gagcgcgacg acgactggaa cgacggccgc
     5761 aaccatgcgc gcaccagccg cgtcgagcgt gtgcagcacg agagtttcaa gagcaaggtc
     5821 acggttcgtt cctcgcagcg cgagtacacg gccaagcagt tcaccaagcc ggcggtccgc
     5881 tcgcacgagt acaaggcggt ttcgaagccg tcaccggcgc gcgtcgagac tcgctcgacc
     5941 acggtcacgc gcgcgaagtc accggtgaag tcgcacgacc gagcccgtgt cgtctcgacc
     6001 tcgagtgcgc gcgagcgcgc gtcggtcgac aaggccgacg tcgccgcgat gaccaagcag
     6061 tcgagccggg cggtgcgccc gtctcgctcg agcgataccg gcaagcgtgc ggtgacaggg
     6121 aagagcgtgc ggacggagtc cacgcgcgag aagggaacga agaagaaaca ggctcgttaa
     6181 atagaagcct cgcggtttgg ttggctggta gcgtcgagta ggctggttag gcaagccgac
     6241 caagtagggg ggagttgcgc cggagtaagc gtcgatcagg ggcggcgaat tacgggcgtc
     6301 aactcccctt tactgttgac cgtcgcgagt gaccttggct agagtaatac gcgttccttg
     6361 ccccgtgacc gaggcccgag tcgtcgggcg gttccgttcc cgagagttgt tccaccgttc
     6421 gcattccgta gaaagatcat cgcattgacc cttcgcatcg gttgttctca cccctgcgcc
     6481 gtgatccgca tggtcgcgtt ggcgaccctg gtcggggcca tgggcgcacg agcggcccag
     6541 ttcgcgccgc cgtatcgcgg ggccgacgcg cggctcacga cccatgcgac gccggcccac
     6601 gtctccgcgc ccgcgtctcc caaccggtac cagcgtgcgc gcatcgaagc catgtcgcgc
     6661 gccaccgccg ccgtcaccga cgacaccttg cgcgtgctcg ccctccaggt ccagttcgcc
     6721 gacagcctga tgggcgggca acccggcagc aaccgcagcg cggtgcgcga ctcgacctgg
     6781 ttcgctaacg agctcattca caccgagcaa tacttccgcg gcgcgtctcg caatcgcttc
     6841 acattcgaat ggacgctcga ggactcgctc tactcgctgc cgcgcgggat ggggtactac
     6901 ggcgccgact cgcgcgaaga agagcgcgtg gtcgagctcg cgcagactct gatcgatttg
     6961 gccgacgcga acgtcgactt ctcgctgtac gaccacgtgt tcatcatcca cgccggcgcg
     7021 gggcaagaga ccgacatcaa cgcggactca cccaaccaga tctggtcgag cttctacgat
     7081 cgcggcgata ttcgcgagat ccaggacgac gacgcttcgc tgggacttcc gaccgacgat
     7141 ctcctgggcg gggatccctt cttcgtcgac aacttctcga tcgtcccttc gcatgcatcc
     7201 caggacttcg ctaccgtggg gacgctcggc atctggacct ttgagatcgg gagccgtatt
     7261 gggctcctgc cgttgttcga ctcgaccccc aacggcgcgc ccgattcgca gggggtgggg
     7321 aacttctgcc tgatggcgta cggcattttc aatgtcaacg gcttcgtgcc cgcgttcccg
     7381 tgcgcgttca atcgcatctt ggcgggctgg ctcgaccctg tggtcgtgga cccggacgac
     7441 acgccggccg cgatccggct ggccgacgtc aacaccggcg ccgacgcgga cacgctgtgc
     7501 gtcaaggtgc cgatcaccga cagcgagtac tacctcgtgg tgaatcgtgt ccatgacgag
     7561 aatttcgaca gcctgttcac ctttgcggat gcggacagcg acctggtgcc ggacaacacc
     7621 gagtcgctcg acggcgccga gttcgatttc ttcctgaccg acctcaccaa ccccttcgag
     7681 cgccgtttcg atccgcgtta cggattcaac gtgttgttcc gttacaccgg cagcggcgtg
     7741 tacgtgtggc acgtcgacga gcgcgtggtc atcgacgcgg cccggcgcgg gtacctcccc
     7801 gacgactacg cggcgcgcaa gggagtcgac ctcgaggagg ccgacggcgt gcaggacatg
     7861 gatcgaccgg ggagtgcggc gttcgcgctc gggagtcact tcgattcata ccgaaccggc
     7921 gacggcaaca accccgtttt cggtcccgac acggatccgg catcgggcac caacgcgggc
     7981 gccgagaccg gcatcacgat cgaaaccctc accgggccgg cgttccgcat gcgcgtctcg
     8041 atcggacgcc ttgttgctta caacgatacg cgcgcgcgct ggtcggcgtc ggcacgcaat
     8101 cagcccgcga ccgtggtgaa tctcgacggc gccggcgacc ccgagatcgt gaccctgggc
     8161 gacaacgcag gtgtgttcgt cctggacgaa tccggccgcg aggtcgtgga tcccgacgcc
     8221 aacccgagca ccatcgcgcc cttcatcgcg gttcccaacg tgacgtggat cgggccgccc
     8281 gcgttcgcga acctcgacgg cgcgcccgac atcgaaatcg tcgcaggtgc gcgcaacggc
     8341 gacgtgtacg tgtggaaggc ggacgggacg ccgttggcgg gagcgccgtt cgcgggcctt
     8401 tccggcgcgg ccgcccccgt gatcgtcgac gtgggtgacg ccgggttgcc ggaaatcctg
     8461 gtcgcgtcgg gcggtaccgg cacgcagctc cgttttatcg acgcgaccgg ggcaccggtc
     8521 gatccacccg ccgccgcggc gttatggccg gccccgttcg acgcgcagat actggcgccg
     8581 ctcgcgatcg ccggcgtttc ggggccgtcg gtgatggggg tggtggcgtg cggcgtcgac
     8641 accacgacct cgcgggtggt ggtgcagtgg acgccgctcg atgccgcgcg cgcgccatgg
     8701 gagcaggcta tcgcgattcc atccgggtgg gacgcggcgt cgttcgtgcc cacggccccc
     8761 gcggtcggcg acgtcgacca cgacggcgac gacgagatcg tgatcgccac gccggacggc
     8821 agcgtcgttg tgttcgacat gtcgtcgact accgtcacgc gcgaaagcgg tgtgctgcgc
     8881 gcccgttatc cgtccgcgcc cgcgctcggc gacgtagacg acgacggcac gctcgagatc
     8941 gcgatctggg acgccgagta catgtacctc ttgaagtcga acgcgcggcc catgctggag
     9001 tggccgcgcg cgatccgcga cgaatcggcg ggtgccgctc ccaccatcag accgcatcga
     9061 gagctggaga gtccgttgtt ggcggacatc gacggcggca ccgtcgacgt ggtgttcccg
     9121 ctggacgacg ggacgctggc tgcgtttcgc gccgacggca ggccggtcgc gtcgttcccg
     9181 cgccccggcc cggcggaggc cggggcaact cccacgctga cggatctcgc cccgggctcg
     9241 ggcagcctgg tcgtgctggg cgcatacggc acgctggacg gcgttgacac ggtggtcgac
     9301 acgctcgcgc atgacccgtc gtcgtcgttg tcgattcagt cgctctcggc gagcgtcaca
     9361 tccccgtatt ggatcatggc gcgtgccgac ctcgcccgta ccgggcgcgt cgtcgagtcg
     9421 gtgccgctcg caacctcgac gagcacattc gacgagggat cgtttatcat ttatcccaac
     9481 ccggtgatgg agagcaccgt gcacgcgcgc atcacgacca acgcccgcgc gcgcgtccgc
     9541 ctctcgatac acactctgga ggggcaggag gcgaccgtgc gcgagttcga cgtcaatccc
     9601 aacgggctcg tcgacacgcc gttcgacgag gccatcgatg tatcgggact gaagagcggc
     9661 atctacttca tgcggctcga gatcgacggc cccggcggca gcggtgcggt ctataagacg
     9721 ttcgcgattc ggcgctaggg cgtcacgagg aaaaggagtc gacaccatgc gtaggcaact
     9781 cgttctggca accctgctcg tcgccgcttt cgtggaaacg gcccacgcgg gcgaccccgg
     9841 cgacgccggg gcgctcttcc tgcgcgtcgg cttcggagcg cgcgcgtcgg ccatgggcga
     9901 ggcgttcacc gccgtggccg aagacgcgac ctcgatctac tggaatccag gtgcgatggc
     9961 ggcggtgctg ggcaccaagg tgacgctggc gcacaccgag tacttcgcca ccatgcgcct
    10021 cgagcaggca tcggtgacgc acgagaccga ctggggcacg atcggattca tgttcacggg
    10081 gctctatatg gacgagatgg atcgctacga ggacaccccc tcggccaacc cgctcggcac
    10141 cttcggcgcc tacgacgtgg cggtctcggc cgcctacgca cgctacctcg tccccaatct
    10201 cgcggccggc atttccatca agccggttta cgagcgcatc gacgagctat cggcctcggg
    10261 gatcgcgttc gacatcgggc tctaccacgt cgcgaggatc caaggggtga agctggccgc
    10321 ggtggcgacc aacgtcggtc cgcccatgaa attcgaccaa gaggagttcg cgttgccgcg
    10381 ctcgatcaag gtgggcggga gctacgagcg cgagatcgcc gcgctcgacg ggcgcattct
    10441 cgtgaccctc gacggcgtgt ttcccaacga cggcgaggtg cgcgagcacg tcggggcgga
    10501 gtactcgtac cgcggaatgc tcgcgttgcg tgcaggctac aaggtgaact acgacgcgca
    10561 ggacgcgacc ttcggcgccg gtttcacctg gcatgagatc accgtcgact acgcgttcct
    10621 gccctcggag aagctcggcg acaaccaccg cgtcgggctc ggcttcagct tttgaacgtc
    10681 tcgatgcgca ggtgtgcgtc ggtcatgcgg acgaaggtgt agtgctcgat ccagtccccg
    10741 acgatcagga actccttgcc ctcccgcgtc tcgtgcatgg gaaagtgcac gtgacccatc
    10801 acgaacccgt cgttgccccg ggaaaagaaa tgatcccacg cgcgtgctgc gaccgcccgc
    10861 gctcgcttct cctgggcgat gtgaaacagc acccggcttc cgtgagccac cccgcccgcg
    10921 atcgcatcca aaatgtcggg gtggatccag cgcgacacct cgatgacgac ggggtttcga
    10981 atgacgttct tcaagacctt gtacccgtag tccctcggca tgacgagatc gccgtgcgcc
    11041 agcacgagct tacggccctg cgcctcgacg caaagctcct ttcggtgcac gcgcacgccc
    11101 agctcgcggg tgaaaaaatc gcccacccag taatcatggt tgccccccag gaagcgggtc
    11161 tcgacgccgc gcgcggtggc gcgtgagata gcggcgaaca cgtcgaggaa gcgccgcggc
    11221 acgaccgacc ggtactcgaa gtagaagtcg aagatgtcgc cgaggaggac gaggaaggtg
    11281 cccttgggcg tcccgtcgag gaaccggatg aagcggtcgc ggcgctccga ttcgccggga
    11341 agcgtccggt cgcggaagtg agtgtcggcg acgatgcgga cgtcggcggc ggcgatgggc
    11401 tcgaacaggt tggctggggt cacgttaagc tctttcgacc gaaaggggga gcatcagcgg
    11461 cgaatcatgc ccgattggca ggatgcatgc aacctgtttg ggcgaggcgg cttgcgcgaa
    11521 ggaacccgcg ccccgactgg gacgtacaat ggagcgcgcg ggccctgagg gatcgcgcga
    11581 cgagtaggca aggttctccg ggtacgcagg ttac
//
LOCUS       DASPGK010000100         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_612417, whole genome shotgun sequence.
ACCESSION   DASPGK010000100 DASPGK010000000
VERSION     DASPGK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612417"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VEC56_02895"
     CDS             <1..562
                     /locus_tag="VEC56_02895"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXV13130.1"
                     /translation="PLDGTTNYIHAYPVFAVTLALEVEGRLMVAVTYGPRDRELFHAV
                     RGHGAFLNDVPMHASRLTDHNRILLGTGFPFRARHLLDLYLKSFAHFFNQARGIRRGG
                     AAALDLAYVAAGRLDGFWELTLSPWDIAAGVLLIEEAGGRVTDFFGGDTYLDSGHVIA
                     TNGLLHEWMCEGIAGIFPRDGNYAKP"
     gene            559..2355
                     /locus_tag="VEC56_02900"
     CDS             559..2355
                     /locus_tag="VEC56_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13131.1"
                     /translation="MTQARALRSLDFWIVLGVALLVRLAYFFLNSRTNPAFDFLIMDS
                     MHIDAWAKAIAAGHAGDAVYFRGPLVPYLLALVYKAGGGVAGAVFVNHLAGAFTCALV
                     WLLAREYFARAVALTAGLGAALYWPAIYFEGEVLIEPVYVALVVLSLWRLARAIARPT
                     TARLLVAGVCVGLAALARPTILALLPAIPFAFSARTAAPSTPPKRAWLRPSLLVAAAC
                     ALVLLPTAIRNYTVGKALVPVTWSGGLNFYIGNNPDSDGRSAMIPGAATPWMGGDDEA
                     LAIARAQAGDSTLTAAAASRFYARRGVGYLATQPRDAASLVAKKFYMFWEGPERSNEK
                     YIYFFWDRFGLGRVPMPGFWLVSPLALAGMIRLWPRRRELALLYLFVVAYTIGVVAFF
                     VVARYRLPVAPVLIVFAAWTAVELTSMVRARRWRAAMSPLALVAALFVAVNASYPEFI
                     ERRPSHIAISHYTLAGAYSDRGDEEGALVELWHARAAYERAPSRYYGEIGQDIYFKLG
                     TLLYQRGKCAEAIKALGEIQPHNPRAHEARFMFAECCEKTGRFPEAGKAYEIVLKTDP
                     ANERALRGYIRCLEAVGRWDDAADARKRLPAE"
     gene            2461..3183
                     /locus_tag="VEC56_02905"
     CDS             2461..3183
                     /locus_tag="VEC56_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198583.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXV13132.1"
                     /translation="MGSRIAAPVLAVGIVLAGWFVGNGFVRGRTADRFVSVKGVSERD
                     VTANMALWPLRFVSTDDNLARAQQKFDESKQVVVAFLGKHAIDASTVELQGFEVTDVL
                     ANPYRGQGEVTSRYIISGTLMVRVEDPALVQKASQDLGSLVQSGVVFSSQGGYNSGPT
                     YLFTKLNDFKPQMIAEATASAREAAEQFAKDSGSRLGPIRTASQGVFQILPRDRAPGV
                     MEESQVDKTVRVVTTVEYTLED"
     gene            3206..>3584
                     /locus_tag="VEC56_02910"
     CDS             3206..>3584
                     /locus_tag="VEC56_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV13133.1"
                     /translation="MTKLASMVVCAALLAGSYSDAEARKSYAGVKGGVNIGDVTGDDA
                     EGLDKRNGFIGGAFYGIDFTDDFGVRLDGLYVQKGAEGPFVAPGDDHAHETVVRLDYL
                     ELPVLCMVGFPTGDSFAVNLFLGP"
BASE COUNT          524 a         1214 c         1202 g          644 t
ORIGIN      
        1 ccccctggac ggcaccacca actacatcca cgcctacccg gtgttcgcgg tcaccctggc
       61 cctcgaagtc gagggccgct tgatggtcgc ggtgacctac gggccgcgcg accgcgagtt
      121 gttccacgcc gtgcgcggcc atggtgcgtt cctgaacgac gtgcccatgc acgccagccg
      181 cttgaccgac cacaatcgga tcctgctcgg cacggggttc ccgtttcgcg cgcgccacct
      241 gctcgacctc tacttgaaat cgttcgcgca cttcttcaac caggcgcgcg gcatccggcg
      301 cggtggtgcg gccgcactcg atctcgccta cgtagcggcg gggcgcctgg acggattctg
      361 ggagctgacg ctctccccct gggatatcgc ggccggcgtg ctgttgatcg aagaggcagg
      421 cggtcgcgtc accgatttct tcggcggcga cacgtacctc gattcggggc acgtgatcgc
      481 gaccaacggg ctgctccacg agtggatgtg cgagggcatc gcaggtatct tcccgcgcga
      541 cggcaattac gcgaagccat gacgcaagcg cgcgctcttc ggtcgctcga cttctggatc
      601 gtgctcggcg tcgcgctgct ggtgcgcctc gcgtatttct tcctcaactc gcgcacgaat
      661 cccgccttcg acttcctcat catggactcc atgcacatcg acgcatgggc caaggcgatt
      721 gcggccggcc atgccggcga cgccgtctat ttccgcgggc ccctggttcc gtaccttctt
      781 gctctggtgt acaaggcggg gggcggtgtg gccggcgcgg tgttcgtgaa ccacctggcg
      841 ggcgcgttca cgtgcgctct ggtctggctc ctggcgcgcg agtatttcgc gcgcgccgtc
      901 gcgctcacgg ccgggctcgg cgcggcgctg tactggcccg cgatctactt cgagggcgaa
      961 gtgctgatcg agcccgttta cgtcgcgctg gtggtgttgt cgctgtggcg cctcgcgcgc
     1021 gcgatcgccc ggccgacgac ggcgcgattg ctcgtggcgg gcgtgtgcgt cggcttggcc
     1081 gcgctcgccc gtcccaccat tctcgcgctc ctgcccgcga ttccgttcgc cttttcggcg
     1141 cgcaccgccg cgccctcgac gccgcccaag cgcgcgtggc tgcggccgtc gctgctggtc
     1201 gcggcggcct gcgcgctcgt cctcttgccc acggcgattc gcaactatac ggtcggaaag
     1261 gcgctggttc cggtgacgtg gtccggcggc ctcaacttct acatcggcaa caatcccgac
     1321 tccgacgggc gcagcgccat gattcccggc gcggccacgc cttggatggg cggagacgac
     1381 gaagcgctcg ccatcgcgcg cgcgcaggcc ggcgattcca cactcactgc ggccgccgcg
     1441 tcgcggttct acgcacgccg cggcgtgggt tacttggcca cccagccccg cgacgcggca
     1501 tcgctggttg cgaagaagtt ctacatgttc tgggagggcc ccgagcggtc caacgaaaag
     1561 tatatctatt tcttctggga ccgattcgga ctgggtcgcg tgcccatgcc cggattctgg
     1621 ttggtgtcgc cgctggcgct cgcgggcatg attcgattgt ggccgcgccg ccgcgagttg
     1681 gcgctgctgt atctgttcgt cgtcgcgtac acgatcggcg tggtggcgtt cttcgtggtg
     1741 gcgcgctacc gcctcccggt cgcaccggtg ctgatcgtgt tcgcggcctg gaccgccgtc
     1801 gagctgacga gcatggtgcg cgcccggcgc tggcgcgccg cgatgtcccc gctggcgctc
     1861 gtcgccgctc tcttcgtggc cgtcaacgcg agctacccgg aattcatcga gcgacggccg
     1921 tcccacatcg cgatttcgca ctacacgctg gccggggcgt atagtgaccg cggcgacgaa
     1981 gagggcgccc tcgtcgagtt gtggcacgcg cgcgcggcct atgagcgcgc gccgtcgcgc
     2041 tactacggcg agatcgggca ggatatctac ttcaagctcg gcacgctcct ctaccagcgc
     2101 ggcaagtgcg cggaagcgat caaggcgctg ggcgagatcc agccccacaa cccgcgtgcc
     2161 cacgaggcgc gcttcatgtt cgccgagtgc tgcgaaaaga ccgggcgctt cccggaagcg
     2221 ggcaaggcct acgagatcgt gctcaaaacc gaccccgcca acgagcgcgc gctgaggggc
     2281 tacattcggt gtctcgaagc ggtgggccgc tgggacgacg cggccgacgc gcgtaagcgc
     2341 ctgccggccg agtagcacgc gcgacaccgg tcgcgcggtt gaccgcgcgc ggattccgct
     2401 ggtagtattc ctttctcgtt tccaatcaac cgtccaaata cgtgtgaacg aggaggtccc
     2461 gtgggttccc gcatcgccgc acccgtgctc gccgtcggca tcgtcctggc cggctggttc
     2521 gtcggcaacg gattcgtgcg gggacgcacc gccgaccgct tcgtgtcggt gaagggcgtc
     2581 tcggagcgcg acgtcaccgc caacatggcc ctgtggccgc tgcgcttcgt gtccaccgac
     2641 gacaacctgg cgcgcgccca gcagaagttc gacgagagca agcaggtcgt ggtcgcgttc
     2701 ctcggcaagc acgcgatcga cgcctccacc gtcgagctgc agggcttcga agtgaccgac
     2761 gtgctcgcca acccttaccg gggacagggc gaggtgacga gccgctatat catcagcggc
     2821 acgctgatgg tgcgggtcga agatcccgcc ctcgtgcaga aggcgagtca ggatctgggc
     2881 tcgctcgtgc agtcgggggt cgtgttctcg tcgcagggcg gctacaacag cggaccgacc
     2941 tacctgttca ccaaactcaa cgacttcaag ccgcaaatga tcgcggaagc gaccgcgagc
     3001 gcgcgcgagg cggccgagca gttcgcaaaa gactcgggca gccgactggg gcccatccgt
     3061 accgcgagcc agggcgtgtt ccagattctc ccgcgcgatc gcgcgccggg tgtcatggaa
     3121 gagtcgcagg tcgacaagac cgttcgcgtc gtcacgaccg tcgagtacac gttggaagac
     3181 tgatcagcgt gaggaaagcc gagcaatgac gaaactggca agcatggtgg tgtgcgcggc
     3241 gctgctggcg ggttcgtatt cggacgcgga ggctcgcaag agttacgcgg gcgtcaaggg
     3301 tggcgtcaac atcggcgatg tcaccggtga cgacgccgag ggactcgaca agcgcaacgg
     3361 gttcatcggc ggcgcgttct acggcatcga cttcaccgac gacttcggcg tgcgcctcga
     3421 cgggctctac gtccagaagg gggccgaggg cccgttcgtc gcccccggcg acgaccacgc
     3481 gcacgagacc gtcgtccgcc tcgactacct ggagctgcct gtcctgtgca tggtcgggtt
     3541 tcccaccggc gactcgtttg cggtgaactt gttcttgggg ccca
//
LOCUS       DASPGK010000101         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_25863, whole genome shotgun sequence.
ACCESSION   DASPGK010000101 DASPGK010000000
VERSION     DASPGK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25863"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VEC56_02915"
     CDS             <1..560
                     /locus_tag="VEC56_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HXV13134.1"
                     /translation="GGLEGRTLRAGDVLQIGAASGSFRKRTVDSRALGEVWPHTIADR
                     AAGEPVRLRVTDGEQSASFSEPARRAFAASVFRVSEQANRMGIRLEGAPIAVPSGGGM
                     VTEGVSLGAVQVPPGGLPIILFVEQQTTGGYPKIANVIAADLASLGQLRPRDAVRFER
                     VQIAAARIALFRQEQLLRSRKTIVE"
     gene            557..1327
                     /locus_tag="VEC56_02920"
     CDS             557..1327
                     /locus_tag="VEC56_02920"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXV13135.1"
                     /translation="MKTIDLNCDMGELPEAVADGTQESLMQSLTSVNVACGGHAGDTR
                     TMQTTIEQALRRRLAIGAHPGYPDRAHFGRKEMKLPPEAVRDFVFEQVRALADVAERC
                     GATLSHVKPHGALYNQAVRDRALAQAIADGVAQCTRDVVLVGLAGSPMLDAFRERGFM
                     VAAEAFADRRYQPDGTLRPRTFDEALIAEPEEAGRQALRIAAHRSVIAHDGTEVPVDA
                     QTICIHGDTPGAVAIAAAVAAAVRGGGVVPRALSTHGD"
     gene            complement(1337..2257)
                     /locus_tag="VEC56_02925"
     CDS             complement(1337..2257)
                     /locus_tag="VEC56_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13136.1"
                     /translation="MLTRLTLLLSFVIASTAPPGFAADIVITAGVDDNFALPADPASP
                     SAALLALPMTFQNFDLIAGVNGLADRNVAHTFTGLPANIQGASLELRVRAGNDAGVNT
                     DGLLISFVDELTTVYCGGAVVYARTFGPFAGGGCFPTADATGLVGSWAVGQTATILLD
                     LSDLPLAGGGGVSVIPEMNSRGFIDVNVSDETGCDFMRLSIDTTQATSVPPRAASVAV
                     LHRPVPNPFNPTTTFRFELVESAQVEFAIYDTAGRRVRTLVAGERPRGTSEVQWDGRD
                     DRGVPAASGVYLGSLRVGPHRLTTKAVLLK"
BASE COUNT          418 a          864 c          924 g          404 t
ORIGIN      
        1 tcggcggtct cgaggggcgg acgctgcgcg ccggcgacgt gctccagatc ggcgcggcga
       61 gcgggtcgtt tcgcaagcgg accgtcgact cgcgcgcctt gggggaggtg tggccgcaca
      121 cgatcgcgga ccgcgcagcc ggcgaaccgg tgcgcctgcg cgttacggat ggggagcaaa
      181 gcgcgtcgtt ttcggagccc gcgcgccgtg ccttcgccgc gagcgtcttc cgcgtgagcg
      241 aacaggccaa ccggatgggg attcgactgg aaggagcgcc gatcgcggta ccgtcgggcg
      301 ggggcatggt gaccgagggc gtttcgctcg gagcggtgca agtgccgccg ggggggctgc
      361 ccatcattct gttcgtcgag cagcagacca cgggcggtta ccccaagatc gcgaacgtca
      421 tcgccgccga cctggcctcg ctcgggcagc tacgaccgcg cgacgcggtg cgattcgaac
      481 gcgtgcaaat agccgcggcg cgcatcgccc ttttccgtca ggagcaactc ctgcgctcgc
      541 gcaagaccat cgtcgaatga agaccatcga cctcaactgc gatatgggcg agttgccgga
      601 ggcggtggcc gatggcacgc aagagtcgct gatgcaatcc ctgacctcgg tcaacgtggc
      661 gtgcggcgga cacgccggtg acacacgcac catgcagacg acgatcgagc aggcgctgcg
      721 ccgcagactc gcgatcggcg cgcacccggg ttatcccgat cgcgcgcact tcgggcgcaa
      781 ggagatgaaa ctcccgccgg aggcggtgcg cgacttcgtg ttcgagcagg tgcgcgcgct
      841 cgccgacgtc gccgagcgct gcggggcgac gttgtcgcac gtcaagcccc acggcgcgct
      901 ctacaaccag gcggtacgcg accgcgcgct cgcgcaggcg atcgccgacg gtgtcgcgca
      961 atgcacccgc gacgtcgtgc tggtcgggtt ggcggggtct cccatgctgg acgcgtttcg
     1021 cgagagaggt ttcatggttg cggcggaggc gttcgcggac cggcgctacc agccggatgg
     1081 gacgctgcgc ccgcgcacct tcgacgaagc gctcatcgct gaacccgagg aagcgggccg
     1141 tcaagcgctg cggatcgccg cgcaccgatc cgtgatcgca cacgacggca ccgaggttcc
     1201 cgtggacgcg cagacgatct gcatccacgg cgatacaccg ggtgcggtgg cgatcgcagc
     1261 ggcggtagcc gcggcggtgc gagggggcgg cgtcgtgccg cgggcgctct cgacgcacgg
     1321 cgactaaggt gcgcccttac ttcagtagca cggccttcgt cgtcagtcgg tgcgggccga
     1381 cgcgcagcga gcccaagtac acgcccgacg cggcgggaac gccccggtcg tcgcgcccgt
     1441 cccattggac ctcgctcgtc ccccgcgggc gttcgcccgc gacgagcgtg cgcacgcgcc
     1501 ggcccgccgt gtcgtagatc gcgaactcga cctgtgccga ctccaccagc tcgaagcgga
     1561 acgtcgtcgt cgggttgaac ggattgggga ccggtcgatg gagtaccgca acactcgccg
     1621 cgcgcggcgg aaccgacgtg gcctgtgtcg tatcgatgct taggcgcatg aagtcgcacc
     1681 cggtctcgtc cgaaacgttc acgtcgatga atccgcggct gttcatctcc ggaatcacgc
     1741 tcactccgcc cccgccggca agcggcaggt cggagaggtc gagcaggatc gtcgccgtct
     1801 gtccgaccgc ccacgacccg accagcccgg tcgcgtcggc cgttggaaaa cagccgcctc
     1861 ccgcgaacgg tccgaacgtg cgcgcataaa cgacggcacc cccgcagtag acggtggtca
     1921 gctcgtccac gaagctgatc aagaggccgt cggtgttgac gccggcgtcg ttgcccgcgc
     1981 gcacgcgcag ctccagcgag gcgccctgaa tgttggcggg cagacccgtg aacgtgtgcg
     2041 cgacgttgcg atccgcgagc ccgttgacac cggcgattag gtcgaaattc tgaaaggtca
     2101 tcggcagtgc gaggagcgcg gcactggggg acgcggggtc cgcgggcaac gcgaagttgt
     2161 catcgacgcc ggcggtgatg acgatgtcgg ccgcgaatcc cggcggggcg gtgcttgcga
     2221 tgacgaagga aagcaagagc gtgaggcggg tgagcatggg gcctccttct gccgcgtgtg
     2281 gcaggtgcgg cgcttggctg cgggacaaga aattgtacgc cccgtcagga tgagtttcaa
     2341 ggtgtcgatg gtgagggatg tctcgccgcg tcgcggtccg ttgccgatgc gcgcggaccg
     2401 gtgttagact tccagagccc cgcgactcgc caaaggagat cgaatcatgc gttgccactt
     2461 gctccgcgtc ctcttcctcc ccgcgctgct cttcgggtgc acgtccgggc tcgaccgccc
     2521 cgtaaccagc gtcgatgcca cgatcgattc cgacggtgtg cagcgcgtca cggttgacat
     2581 gcacagctac tacttcgagc ccaatcgcat
//
LOCUS       DASPGK010000102         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_963414, whole genome shotgun sequence.
ACCESSION   DASPGK010000102 DASPGK010000000
VERSION     DASPGK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963414"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1909
                     /locus_tag="VEC56_02930"
     CDS             260..1909
                     /locus_tag="VEC56_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13137.1"
                     /translation="MASFLPGGTATSDRIDDLAAELTLSLELSRGEGCDLAATQRAAD
                     RLADAFHAAIVAAEEAERDPTNASLDDVYKLVESAYRQWKGVDAAFAQTLRLAYEHQV
                     PRTNLLIDDIRASLERRVGEPIAEAYTELRTRWAAHVDSRPWDSFLNRAPQHVDRGPL
                     EGALNALARGDDLDVEDAIGSLANGLRYAFAEHLHTHPEDLDDIEVGIWKRPEVLVAS
                     DFWGHPKRTRLIDVLAHSGSDRSRKTAVTLAGLFAPNPKGAAAVVRALDDVHESQRER
                     FTRCLMLHPDYEVRRYAANNTDINSIWKVVTPDAVPCATILSLLERLVGSSRYTTAQR
                     KIFFDAVYRRLLSLTTRSDVLYARGIVRIFVKLNFFLEDSYFYKLSTLMDYLASKEKL
                     HGINDGLLAEYIARMRAEKTRVGSVPGEDPSFDGIPLVVLRKLARDGHFWHVLITHPI
                     AKIARETVVHVSTKDRALQIANNHRVNSEVLRGLGRKRELFSSLPAKMALLSNPQTPI
                     AVSMNYVSDLTLQDVEALLRRSTVHPELRNQLRAKYDAVRR"
     gene            1973..3313
                     /locus_tag="VEC56_02935"
     CDS             1973..3313
                     /locus_tag="VEC56_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXV13138.1"
                     /translation="MHANLGEVLSVWWVIPFVGILLSIALFPLFAPRFWHHHFPKVSA
                     FWALALAVPFVWFFQNAALHEIAHIFLVDYIPFIILLWALYTVSSGILLTGTLDGSPR
                     QNTLIIASGTLIASFIGTTGASMVLIRPLLRAISWRRHKAHIVVFFIFLVSNIGGSLT
                     PLGDPPLFLGFLHHVPFFWTFNLAPAMLFMAVPLLAVFYLIDRRYHAREEKAPERASA
                     VMGIRGWQNFLCLGGIVAAVLMSGVWKLGSVHVLGVEMKIEGILRDVLLIVLGVTSLK
                     TTSPAIHKDNQFSWFPIKEVAILFAGIFMTIIPALAILKAGTHGALKPLVELADQPES
                     YFWLTGVLSSFLDNAPTYLTFFNTALGALAPGLPEPQAVQQLIHMHGHYLHAISCGAV
                     FMGANTYIGNAPNFMVKSIAEEAGVRMPSFFGYLFKYSIPFLIPLFLIATFIFF"
     gene            3336..>3781
                     /locus_tag="VEC56_02940"
     CDS             3336..>3781
                     /locus_tag="VEC56_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13139.1"
                     /translation="MATYYELVIKGDDRDLVPYLAGYLAGAAVDGVYFAGESGLHVQA
                     MRERIRHHGEVVHVVCAKKVLAAVREALQEAAPRYRFEIKEERAIASAHFRFKVETPS
                     RKVAEAVKEAVAKPGRGVEVKGFEPRESVDPDKTGAEVYSPVHDYT"
BASE COUNT          633 a         1271 c         1175 g          702 t
ORIGIN      
        1 cgcgcgcggg tcctgtccgc caacagccca ctgcgctcgc aaccggtcgg cgcgatcgat
       61 tcctcgacgc gatttctgac gcgcgaatac gacaccgtat tgtgccgttc gcgcgtcagt
      121 ttgagcggag aggaacgatc gcgccggcca aacgcctctc caaggcacgt cgcttgcgat
      181 atcccacctc gaagcaccgt agcgattcgc tcaagttgcg aatcgccaac gggttcctgc
      241 ttcgggaggg gaatcgcgca tggccagttt cttgccgggc ggcacggcga catccgatcg
      301 aatcgacgat cttgcagccg agctcacgct ctcgctcgag ctttcgcggg gcgaagggtg
      361 cgacctcgcc gcgacacagc gcgccgccga ccggctggcg gacgcgttcc acgcggccat
      421 cgtggccgcc gaggaagccg agcgcgaccc gaccaacgcg tcgctcgacg acgtgtacaa
      481 gttggtcgag tcggcgtacc gccagtggaa gggcgtcgac gccgccttcg cgcaaaccct
      541 tcgtctcgcc tacgagcacc aggtgccgcg caccaatctc ttgatcgacg acattcgcgc
      601 ctcgctcgag cgccgggtgg gcgagccgat cgccgaggcg tacaccgaat tgcgtacgcg
      661 ctgggccgcg cacgtcgact cgcgcccgtg ggactcgttc ctgaaccgcg cgccgcagca
      721 cgtggatcgg ggcccgttgg agggcgcctt gaacgcgctc gcgcgcggcg acgacctcga
      781 cgtggaagac gcgatcggtt ccctcgcgaa cgggctccgc tacgccttcg cggaacattt
      841 gcacacgcac ccggaggacc tcgacgacat cgaagtggga atctggaagc gccccgaagt
      901 attggtcgcc tccgacttct ggggacaccc caagcgtacg cggttgatcg acgtactcgc
      961 gcacagcggc tcggaccgct cgcgcaagac cgcggtcacg ctggccggtc tcttcgctcc
     1021 caatccgaag ggcgcggcgg cggtggtgcg cgcgctcgac gacgtccacg aatcgcagcg
     1081 cgagcgcttc acgcgctgcc tgatgctgca cccggactac gaggtgcgcc gctacgcggc
     1141 caacaacacc gacatcaact cgatctggaa ggtcgtgaca cccgacgcgg tcccgtgcgc
     1201 gaccatcctg tcgctgctcg agcgcctggt gggatcgagc cgctacacta ccgcccagcg
     1261 caagatcttc ttcgacgcgg tctatcgccg gctcttgtcg ctcacgacgc gctccgacgt
     1321 gttgtacgcg cgcggtatcg tgcgcatctt cgtgaagctc aatttcttcc tggaagactc
     1381 gtacttctac aagctctcca cgctaatgga ctacctcgcc agcaaggaga agctgcacgg
     1441 catcaacgac gggctcctcg ccgaatacat cgcgcgcatg cgcgcggaga agacccgggt
     1501 gggctcggtg cccggagagg acccgtcctt cgacggcatc ccgctggtgg tgctgcgcaa
     1561 gctcgcgcgc gacgggcatt tctggcacgt gctgatcacc cacccgatcg ccaagatcgc
     1621 gcgcgagacg gtggtgcacg tctcgaccaa agaccgcgcg ctgcagatcg ccaacaacca
     1681 ccgcgtgaac tcggaggtgc tgcgcgggtt gggccgcaag cgcgagctgt tctcgtcgct
     1741 gccggccaag atggcgctgc tttcgaatcc gcagaccccg atcgcggttt cgatgaacta
     1801 cgtctccgac ttgacgctcc aggacgtcga ggcgctcttg cgccgctcga ccgtccatcc
     1861 ggaactgcgc aaccaactgc gcgcgaagta cgacgccgtt cggcgctgac gccgccgcgc
     1921 ggccattttc gcttgcgctg gggggcgacc atgccgcact ctaggcggca ttgtgcacgc
     1981 aaacctcggc gaagttctct cggtctggtg ggtcatcccg ttcgtgggca tcttgctctc
     2041 gatcgcgctg ttcccgctgt tcgcgccgcg cttctggcac caccacttcc ccaaggtgtc
     2101 ggcgttctgg gccctcgcgc tcgcggtccc gttcgtctgg ttcttccaaa acgcggcctt
     2161 gcacgagatc gcgcacatct ttctggtcga ttacattccg ttcatcattc tgttgtgggc
     2221 gctctacacg gtctcgtcgg gcatcctgct gacgggaacg ctcgacgggt ctcccaggca
     2281 gaatacgctc atcatcgcgt ccggaacgct gatcgcgtcg ttcatcggga ccacgggcgc
     2341 gtcgatggtc ctgatccgcc cgctcctgcg cgcgatctca tggcgacggc acaaggcgca
     2401 catcgtcgtc tttttcatct tcctggtgag taacatcggc ggttcgctca caccgctggg
     2461 cgatccgccg ttgttcctgg gcttcctgca ccacgtgccg ttcttttgga ccttcaacct
     2521 ggcgcccgcg atgctcttca tggcggtccc gctgctcgcg gtcttctatc taatcgaccg
     2581 gcgctaccat gctcgcgaag agaaggcgcc ggagcgcgcg tcggcggtca tgggcatccg
     2641 agggtggcag aatttcctgt gcttgggcgg gatcgtggcg gcggtcctga tgagcggcgt
     2701 gtggaagctg gggagcgtcc atgtgctggg cgtcgagatg aaaatcgaag gcatattgcg
     2761 cgatgtcctc ctgatcgtcc tcggcgtcac gtcgctcaag acgacgtcgc ccgcgatcca
     2821 taaggacaac cagttctcgt ggttccccat caaggaagtc gcgatcctct tcgcgggtat
     2881 cttcatgacc atcatccccg cgctcgcgat tttgaaagcg ggtacacacg gggcgctgaa
     2941 acccctggtc gagctggccg accagcccga gagctacttt tggctcacgg gggtgttgtc
     3001 gagcttcctg gacaacgcgc cgacctatct cacgtttttc aacaccgcgc tcggcgcgct
     3061 ggcccctgga ctcccggagc cgcaagccgt gcaacagctg atccacatgc acggacacta
     3121 cctgcacgcg atctcgtgcg gcgccgtgtt catgggcgcc aacacctaca tcggcaacgc
     3181 gcccaacttc atggtgaaga gcatcgccga ggaagcgggc gtgcggatgc cgagcttctt
     3241 cggctacttg ttcaaatact cgattccgtt cctgattccg ctgttcttga tcgcgacctt
     3301 catcttcttc tgatgagctc gcgacggagg tcgtcatggc gacatactac gaattggtga
     3361 tcaaaggcga cgaccgcgac ctcgtgccct atctcgccgg ctacctggcg ggcgcggccg
     3421 tcgacggggt ctacttcgcg ggggaatccg gcctgcacgt gcaggcgatg cgcgaacgca
     3481 tccgccacca cggcgaagtc gtgcacgtcg tgtgtgccaa gaaggtgctc gcggccgtgc
     3541 gcgaggccct ccaagaggcg gcaccgcgct atcgcttcga gatcaaagaa gagcgcgcga
     3601 tcgcgagcgc gcactttcgc ttcaaggtgg aaacgccgag ccgcaaagtc gcggaagcgg
     3661 tgaaggaagc cgtggcgaag cccgggcgcg gggtcgaggt gaagggtttc gaaccgcgcg
     3721 aatccgtcga ccccgacaag acgggagcgg aagtctactc gcccgtgcac gactacacgt
     3781 t
//
LOCUS       DASPGK010000103         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_378312, whole genome shotgun sequence.
ACCESSION   DASPGK010000103 DASPGK010000000
VERSION     DASPGK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378312"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(703..1572)
                     /locus_tag="VEC56_02945"
     CDS             complement(703..1572)
                     /locus_tag="VEC56_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13140.1"
                     /translation="MLALRARMGYGRDVAMFTITRKQSFNEDTFLLEFRLPTIAERAM
                     PGQYVDIHMNVDGPTFTLPIATIDREAGTFTVVEQARDLATERLMMLAQGEEVFQIRG
                     PLGAPCRIDSDGKVVLIAENLGAASLLWRARAFKEHGAHVIVVLGFDSKDRVFWKDEF
                     AAVADELYVATEDGSFGVSGRVTGVLQALCETHKDIDRIGVVANLKHMKRAAKIASDF
                     GIDCTVSFDAIRFPAGAPTIFDHADDAQETFAFARAPELDAESVDFEKLLSRERALSS
                     NTESQPGSSRAIG"
     gene            1734..2774
                     /locus_tag="VEC56_02950"
     CDS             1734..2774
                     /locus_tag="VEC56_02950"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXV13141.1"
                     /translation="MKDTMHKADLNWSELPFDYVRTDCHLQYVYRDGKWDEGTVCEDD
                     KVTISVASTCLHYGQECFEGLKVYETKNGHALAFRPDENAKRMAHTAKKALMVAPPVD
                     LFVDAVDRVTKLNRRFIPPYGSGASLYIRPLLIGITGTIGIKPSREYMLAVLATPVGP
                     YFKTGLKPVRLWVEEEVDRAAPLGVGDAKLGGNYTAGLRATYGARDRGYAEVLFLDSK
                     QHKYIDESGSSNFYAITKDGRYVTPKSSSILPSITNKSLMDVARDLKIPVDERPVEVA
                     ELPNFAEAGLVGTATIISPVYAIQHGKKLIQYGGPNDVGPVSMKLRDTLLAIQTGDAP
                     DTHGWTREIQLD"
     gene            2842..>3782
                     /locus_tag="VEC56_02955"
     CDS             2842..>3782
                     /locus_tag="VEC56_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXV13142.1"
                     /translation="MNTIEKQQLTLTCHVDSYRSGWSIVEFLAHRFPYHTREGWTTRV
                     VDHWVRVNDAVVPPDHVVRKNDAVAYTILHAEPPVDDRYEVLFEDDHYLAVAKSGNIP
                     VHACGIYIVNTLIARLRADFGAGVNLVHRLDRETSGVVVLAKHRDANRLLARMFERGE
                     VEKGYLAIVHGHIAERAFVIDAPIGKIDARFQYPVEYAWGKENDLATYLPKRRVDPEG
                     KPARTRGEVSQYVGRFTILRVLPEQGRTNQIRVHLAHAGHPIVGDKIYSLSGDVREEQ
                     LREGLTDRVRAALVLPRHALHCESLAFTHPLDGARVEL"
BASE COUNT          716 a         1181 c         1207 g          678 t
ORIGIN      
        1 tcgacatcgc ctccactcta gtgtaagata cacgtgggtt ggaaggttct gcagaaggct
       61 acgggaaacg catgaacccg gtcaatatcc gataccgttt ccgcgccgtg gcgagcaccg
      121 ccggtctatt ctgctgctcg ctcgtctccg acccgcgcgc agcagacatc acagttctcg
      181 gtgctgagcc atgggatcag gtcacacttt cccagcaaag aggcgatttc gtgaagccta
      241 cttctacttc ggtaccgccc ccgttactac agtgcaaccg acaagtgtcg cgccactggg
      301 agcgcgtctg gcacccaacc catttggcga ttcagcaact cttcagttcc agcttgcgac
      361 gccttcggaa gtaacacttc gcgtctacga cgttcggggt cgtctagtcg acgcgcaata
      421 ctatggcacg ctgggcgcgg gtgaccagga gttacgctgg ccggcagccc ctggcgcgtc
      481 ttccctagct agcgggatct actttgcgcg tctgcaggtc ggtaatcgta tccaaactgt
      541 ccgaatgcac cggattcgat aaggacgctc gcgaaacggt cccttgattt ctccgggttc
      601 cgtggagtgg ggacggcgcg gacaagtcga atcaacttcc atcggggatt tacttcctgc
      661 ggttgacgac tccctcggct accgtcacgc gtaagatggt gctcatccga tagctcggct
      721 agagccaggc tgggactcgg tattgctgct caacgcgcgc tcgcgcgata gcagcttctc
      781 gaagtcgacc gactccgcgt ccagctcggg ggcgcgcgcg aatgcgaagg tctcttgcgc
      841 gtcgtcggcg tggtcgaaga tggtgggtgc gccggcgggg aagcggatgg cgtcgaagct
      901 cacggtgcag tcgatgccga agtcgctggc gatcttggcg gcgcgcttca tgtgcttcag
      961 attggcaacc acaccaatac gatcgatgtc cttgtgggtc tcgcacaagg cctggaggac
     1021 gccggtcacg cgcccgctca cgccgaagga gccgtcttcg gtggcgacgt agagctcgtc
     1081 ggcgacggcg gcgaattcgt ccttccagaa cacgcggtct ttggagtcga agccgagcac
     1141 gacgatcacg tgcgcaccgt gctccttgaa cgcgcgcgcg cgccacaaca aggacgccgc
     1201 gcccaagttc tccgcgatca agaccacctt gccgtcgcta tcgatgcggc acggcgctcc
     1261 caacggcccg cgaatctgaa acacctcttc gccctgggcg agcatcatga gtcgctcggt
     1321 ggcaagatcg cgcgcctgct cgacgacggt gaaggtgccg gcttcgcggt cgatggtggc
     1381 gatggggagg gtgaaggtgg gcccatcgac gttcatatga atgtcgacgt actggccggg
     1441 catcgcgcgc tcggcgatcg tgggcaggcg gaactcgagg aggaaggtgt cctcgttgaa
     1501 ggattgcttg cgcgtgatgg tgaacatggc cacgtcgcga ccgtacccca tgcgggcgcg
     1561 gagggcaagc acggagtcgc ccgtggaaac cgcatgagga cgcctcattc gtcctgtgga
     1621 caaatcgtgc cgtgggggcg aaagcgattg acacccggcc cgcgcggcgg gtattttgga
     1681 ggcgtccatc cgacggttcc gccgcaattc tccatccact aggacgtgcc gcgatgaagg
     1741 ataccatgca caaagcggat ctcaattgga gcgaactgcc gttcgactac gttcgcacgg
     1801 attgccattt gcagtacgtc taccgcgatg gcaagtggga cgaaggaacg gtctgcgagg
     1861 acgacaaggt cacgatctcg gtcgcctcga cgtgtctcca ctacggccag gaatgcttcg
     1921 aaggtctcaa ggtctacgag accaagaacg ggcacgcgct tgcgttccgc cccgatgaga
     1981 atgcgaagcg catggcgcac acggccaaga aggcgctcat ggtggcgccc ccggtggacc
     2041 tcttcgtcga cgcggtggac cgcgtcacca agctcaaccg ccgtttcatt ccgccctatg
     2101 ggagcggtgc gtcgctctac attagaccct tgctgatcgg catcacgggc accatcggta
     2161 tcaagccgtc gcgcgagtac atgctcgcgg tgttggcgac accggtgggg ccgtacttca
     2221 agaccggatt gaagccggtg cgcctgtggg tcgaggaaga agtcgaccgc gcggccccgc
     2281 tcggcgtggg cgatgcgaag ctcggcggca attacacggc cggactgcgc gcgacctacg
     2341 gcgcccgcga ccgcggctac gccgaggtgc tgttcctcga ttcgaagcag cacaagtaca
     2401 tcgacgagtc gggcagctcg aacttctacg cgattaccaa agacgggcgc tacgtgacgc
     2461 cgaagtcgtc gtcgattctg cccagcatca ccaacaagag cctgatggac gtcgcgcgcg
     2521 acctgaagat tccggtcgac gaacggccgg tcgaagtagc ggagcttccg aactttgcgg
     2581 aagcggggtt ggtcggcacg gccactatca tctcgccggt gtacgcgatc cagcacggca
     2641 agaagctcat tcagtacggc gggccgaacg acgtgggtcc ggtgagcatg aaactccgcg
     2701 acacactcct ggcgatccag accggcgacg ctccggatac gcacggctgg acgcgggaga
     2761 ttcagctcga ctagaacgag cccgcgagat cccgatcacg aaggctcctt cgcgtcggag
     2821 gggccttcgt cgtttgaacg agtgaatacc atcgagaagc aacagctcac gctcacgtgc
     2881 cacgtcgact cgtaccgctc cggctggtcg atcgtcgagt ttctcgcgca ccggttcccg
     2941 taccacacgc gcgagggctg gacgacgcgc gtcgtcgatc attgggtgcg cgtgaacgac
     3001 gcggtggttc cgcccgatca cgtcgtacga aagaacgatg cggtggcgta caccattctg
     3061 cacgcggagc cgcccgtcga tgaccgctac gaggttctgt tcgaggacga ccattatctc
     3121 gcggtggcga agtcgggcaa catccccgtc cacgcgtgcg ggatctacat cgtcaatacg
     3181 ctgatcgcgc ggctgcgggc ggacttcggc gccggcgtca acctggtgca tcgcctggac
     3241 cgcgagacca gcggcgtcgt cgtactcgcc aagcaccgcg acgccaaccg cctcctcgcg
     3301 cgcatgttcg agcgcggcga ggtggagaag ggctatctcg cgatcgtgca tgggcacatc
     3361 gccgagcgcg cattcgtcat cgacgcaccc atcggaaaga tcgacgcgcg cttccagtat
     3421 ccggtcgagt acgcgtgggg gaaggagaac gatctagcaa cgtaccttcc caagcgtcgc
     3481 gtcgatccgg aggggaaacc cgcgcgtacg cgcggggaag tatctcaata cgtgggtcgc
     3541 ttcacgatcc tacgagtact cccggagcag ggacgaacca atcagattcg cgtccacctc
     3601 gcgcacgccg gccacccgat cgtgggagac aagatctact ccttgagcgg agacgtgcgg
     3661 gaagagcagc ttcgagaagg actgaccgat cgcgtgcgcg cggcgctcgt tctcccgcgc
     3721 cacgcgctcc actgcgagtc gctcgcgttc acgcacccgc tggacggggc gcgcgtcgag
     3781 ct
//
LOCUS       DASPGK010000104         8110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1081806, whole genome shotgun sequence.
ACCESSION   DASPGK010000104 DASPGK010000000
VERSION     DASPGK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081806"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VEC56_02960"
     CDS             complement(<1..942)
                     /locus_tag="VEC56_02960"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HXV13143.1"
                     /translation="MRRSPRFLLLCTIAALTAGCSDTNQPTSTTPNPQTNFALSPMGE
                     GCASIGDRVWLDENCNGIQDKRDDDSPEVGVAGVVVELYTCDGSLVASTTTDATGYYL
                     FEGLDEYGQFKVCFVLPDGYSFTKPNQGTSEGLDSDAGDDGCSDCIDFVDCKPIRYVD
                     AGLCRDESTGCRVTGGGVDEFGNWDGSTTNGRCHNDRYTFGGQAGANTALPPQPAGEW
                     EHNNHSGPSGSFAFHGGTHSAPDGTEIDRIECSDAGFCDPARHAPAKQIDFWGVGMFH
                     NARNLSPILAANVVVSESLHWFEVNIDDGGEPGRGKGG"
     gene            complement(1119..1964)
                     /locus_tag="VEC56_02965"
     CDS             complement(1119..1964)
                     /locus_tag="VEC56_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV13144.1"
                     /translation="MSLTLLSLILSAAILHATWNFFAKRSGGGLTVLWLGAAISTVAT
                     IPAAVAAQWGKPIARDGLVIGCVSGLVHCAYWWALARMYEAGDISLAYPIARGSGVAG
                     TAIGSIVWLREPLSFAGAMGIGCVCVGVAALGYQRRVEAVRVRAVALALITGLTITGY
                     TLLDDRGVETMSPPTYLAIETGVGAAALALLMRRRLKAGLFSAYRTHWKTAWIIGIGS
                     PLTYLIILVAYAAGPVGYITAVREFSVVIAALLGVWFLGERMSIARWVGIALVVAGMV
                     LIKGA"
     gene            complement(1983..5372)
                     /locus_tag="VEC56_02970"
     CDS             complement(1983..5372)
                     /locus_tag="VEC56_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXV13145.1"
                     /translation="MKPIDPYQPKHKVRIVTAASLFDGHDAAINVMRRIIQSTGCEVI
                     HLGHDRSVEEVVDAAIQEDAQAIAMTSYQGGHIEYFKYMKDLLEQRGASHIRIFGGGG
                     GVILPHEIEELHQYGIARIYSPDDGRAMGLQGMINDLVMKSDFATGVSLNGAVDGVLK
                     RDFGSIAKTISAAENAPKESERDLAKVRARAKESKAPILGITGTGGAGKSSLVDELVR
                     RFLIDFPDKTVGVVCVDPSKRKTGGALLGDRIRMNAIRSKRVYVRSLATRQSNLALSA
                     HVKDAIDVLKAANFDLILLETSGIGQSDTEIVDHSNVSLYVMTPEYGAASQLEKIDMI
                     DFADLIALNKFDKRGAMDALRDVRKQYKRAHKKFDLDDEKTPVFGTIASQFNDPGTNN
                     LYRAVVDALVAKCGLPAEKWRSSFDLTAEQSEKIYIIPPPRTRYLSEISETCREYDKW
                     VNEQSGVAQKLFALRTSIETVRAGKAADKDRIVKDLESTYAEIARDLAPENQDIIAGW
                     DEKKKRYREPLYTFQVRGKDISIQTHSESLSHTQVPKIALPRYCSWGDVLRWNLSENV
                     PGEFPYAAGIYPFKRTEEDPTRMFAGEGAPERTNRRFHYVSYHMPARRLSTAFDSVTL
                     YGRDPDTRPDIYGKIGNSGVSVCCLDDAKKLYSGFDLADPKTSVSMTINGPAPMILAY
                     FLNAAIDQQCELYIKKNGLEGDVKKKIDAIYQGRDALRPRYAGELPEGNEGLGLLLLG
                     VTGDEVLPREVYDRIKANTLRAVRGTVQADILKEDQAQNTCIFSTEFALRLMGDVQDH
                     FIRNQVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTYVEYYLSRGMDINKFGPNL
                     SFFFSNGIDPEYAVIGRVARRIWAKAMKHKYGADERSQMLKYHIQTSGRSLHAQEIAF
                     NDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNEN
                     PLQGAFIVEELTDLVEEAVLLEFDRITERGGVLGAMETMYQRGKIQEESLYYETLKHS
                     GDLPVIGVNTFLSDEGSPTILPREVIRATEDEKKRQIATVSALHARAGARAHDALRAI
                     QRVAVRNENVFDQLMEAAKVCSLGQTTQALFEVGGEYRRNM"
     gene            complement(5449..6102)
                     /locus_tag="VEC56_02975"
     CDS             complement(5449..6102)
                     /locus_tag="VEC56_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13146.1"
                     /translation="MTRIVVTGIVLTLFAASAAAQEARRSSWGFYAAPTSHHVSSGTD
                     LETMESSTSLDWGLFYARTFTRAMVRIEARYASREFYGSYTSALFPTGFPVQPMREDF
                     LELPIVLHGTNTVSLNDATIRISIGGGLYYALLISQEFLGGPSSLPDAPEELGAGDYS
                     RYGWLGDGGVALIMKERNAVFLHFRMQSDLGVTGEPEQSIAREDAAFGFYAGFEWLF"
     gene            complement(6099..6575)
                     /locus_tag="VEC56_02980"
     CDS             complement(6099..6575)
                     /locus_tag="VEC56_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13147.1"
                     /translation="MKQKVVGRKGIDVTIVWAPMMEGDDEAAAREAARMFDKKHVAHF
                     YDPERRVGLAYRREVFPNAYDEALASLPADHWLRDVLLERGREYGDRPEWDIYMFFDR
                     GAKWMAAPPRPIHFVRHLGRVGENGESLMWVDGYGREPVEGDLANEIQRLAREVAK"
     gene            7002..>8110
                     /locus_tag="VEC56_02985"
     CDS             7002..>8110
                     /locus_tag="VEC56_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HXV13148.1"
                     /translation="MRPVADPEPSGGATAAAEQLRAGPRVVSGDPEANPPLGASADGF
                     NFDTNGVLTGNLFIPPDPHCAAGPDHVVMVGNVAIEWRPKDPLAAAPQVLMSLQSFFS
                     ALPGPPPAPGTTLGSFTFDPKVIYDQYRQRFIAVALERWDVAFGNPSNQSRILVAVSR
                     GPDPNLGWWFHSIDSKLLIGAFEHWADYPGLAVGDDAVYITNNMFRFVAGGSNFGGVR
                     LWIIDKVPTYAGPNNSIAFTVHDPYAAAGIATTTQPAHTFGPTGAGSTGFHLGTFLLS
                     YSGITDGVNEAVQVVEVTDPLGAVGGPFFVQQFVNFGNIDNTAVVTLPDAPQAGGAPA
                     IEVNDRRALNAVWRSNNIWTCAELVPVAGADVGQTT"
BASE COUNT         1391 a         2602 c         2600 g         1517 t
ORIGIN      
        1 gcctcccttt ccgcgaccgg gctcgccgcc gtcgtcgatg ttcacttcga accagtggag
       61 cgattcgctc acgacgacgt tcgccgccag aatcggtgac aggttccgcg cgttatggaa
      121 catgccaacg ccccagaaat cgatctgctt ggccggcgca tgacgcgcgg gatcgcagaa
      181 cccggcatcg ctgcactcga tgcggtcgat ctcggtgccg tccggcgcgc tgtgggtccc
      241 gccgtggaat gcgaagctgc cgctgggacc actgtggttg ttgtgctccc attcgccggc
      301 cggttgcggc gggagcgccg tgttggcgcc cgcctggccg ccgaacgtgt agcggtcgtt
      361 atggcagcga ccgttggtcg tggaaccatc ccagttgccg aactcgtcga cgccaccgcc
      421 ggtcacccgg cacccggtgg actcgtcgcg gcacagaccg gcgtcaacgt accggatcgg
      481 cttgcaatcg acgaaatcga tgcagtccga gcacccgtcg tcgcccgcat cgctgtcgag
      541 accctcgctc gttccctgat tcggtttcgt gaaggaataa ccgtcgggca ggacgaagca
      601 cactttgaac tggccgtact cgtcgaggcc ttcgaacagg tagtagccgg tcgcgtcggt
      661 cgtcgtggag gcgacgaggc tgccgtcgca ggtgtagagt tctaccacca cgccggctac
      721 gccgacttcc ggcgagtcgt cgtcgcgctt gtcctgaata ccgttgcagt tttcatcgag
      781 ccagacgcgg tcgccgatcg acgcacaccc ctcgcccatc ggcgagagcg cgaagttcgt
      841 ctgcggattc ggcgtcgtgc tggtaggctg attggtgtcg ctacatcccg ccgtcagcgc
      901 ggcaatggta cacaatagga ggaaacgggg gctacgtcgc atggctgcac tcccatgttg
      961 gggcgttgtt atgttggggg gctgggcggt acttttaccc gcacccgcgg ataggctgct
     1021 caaacgagca aactacagtt tagcgcaggc gatgacccga ggggaagccc aaaaatggcg
     1081 ttcccaggct aaatctcgcc gatttggggc cggcgccgtc aggccccttt gatcaggacc
     1141 atcccggcca cgacgagcgc gatcccgacc caacgcgcga tcgacatgcg ctcacccagg
     1201 aaccacaccc ccaagagggc ggcgatcacc accgagaact cccgcacggc tgtgatgtac
     1261 cccaccggcc ccgcggcgta cgccacgaga atgatcaagt aggtgagcgg cgaaccgatt
     1321 ccgatgatcc acgccgtctt ccagtgggtg cggtacgcgg aaaacagccc agccttgagg
     1381 cgcctgcgca tcagcagcgc cagcgccgcc gcgccgaccc cggtctcgat ggccaggtac
     1441 gtcggcggcg acatcgtctc cacgccacgg tcgtcgagga gcgtgtaacc ggtgatggtc
     1501 agccccgtga tcagcgcgag tgccaccgcc cgcacgcgga ccgcttcgac gcgccgctgg
     1561 tatccgagcg cagccacgcc cacgcacacg cacccgatac ccatggcacc cgcgaacgac
     1621 agcggctcgc gcagccacac gatcgatccg atggccgtac ccgccacccc gctcccgcgc
     1681 gcgatcggat acgcgagcga gatatcgccg gcctcgtaca tgcgtgccag ggcccaccag
     1741 taggcgcagt gcaccaggcc gctcacacaa ccgatgacga gcccgtcgcg cgcgatgggc
     1801 ttgccccact gcgcggcgac ggccgcgggg atggtcgcga cggtcgagat agcggcgccg
     1861 agccagagca cggtgagtcc cccgccggat cgcttggcga agaagttcca cgtggcgtgg
     1921 agaatggcgg cggaaagaat gagcgagagg agggtgaggg acatgatcga cgcgggaggg
     1981 tatcacatgt tgcgccgata ctctcctccc acctcgaaca gcgcctgcgt cgtctgtccc
     2041 aacgagcaca ccttggccgc ttccatgagt tgatcgaaaa cgttctcgtt gcgaaccgcg
     2101 acgcgttgga tcgcgcgcag cgcgtcatgc gcgcgcgcac cagcgcgcgc gtgtagtgcg
     2161 ctgactgtgg caatctgacg tttcttctcg tcttcggtcg cgcgaatgac ctcccggggg
     2221 agtattgtgg gcgacccctc gtcggagagg aatgtgttga cgccgatcac cggcagatcg
     2281 cccgagtgct tgagcgtctc atagtaaagc gactcttctt gtattttgcc cctctggtac
     2341 atggtttcca tggcgcccaa gacgccgccg cgctcggtga tgcggtcgaa ctcgagcagc
     2401 accgcctctt cgaccaagtc ggtgagctct tccacgatga aggcgccctg gagggggttc
     2461 tcgttcttgg ccagccccaa ctccttgttg atgatgagct ggatcgccat cgcgcgccgc
     2521 accgactctt cggtgggcgt ggtgatggcc tcgtcgtagg cgttcgtgtg gagcgaattg
     2581 cagttgtcgt agatggcgta gagtgcctgc agcgtggtgc gaatgtcgtt gaacgcgatc
     2641 tcctgcgcgt gcagcgagcg gcccgaggtc tggatgtggt acttgagcat ctgcgaacgc
     2701 tcgtcggcgc cgtacttgtg cttcatcgcc ttggcccaga tacggcgcgc gacccggccg
     2761 atcaccgcgt actcggggtc gatgccgttc gaaaagaaga acgacaagtt gggaccgaat
     2821 ttgttgatgt ccatcccgcg cgacaaataa tactcgacgt aggtgaaccc gttggcgagc
     2881 gtgaaggcga gctgcgagat cgggttggca cccgcctccg cgatgtggta gcccgagatc
     2941 gacaccgagt agaaattgcg cacctggttg cgaatgaagt ggtcctgcac atcacccatc
     3001 agccgtagtg cgaattcggt ggagaagatg caggtgttct gggcctggtc ttctttcaat
     3061 atgtcggcct ggaccgtccc acgaaccgcg cgcagggtgt tggccttgat gcggtcgtac
     3121 acctcgcgcg gcagtacctc gtcgccggtg acaccgagca gcaggagccc caagccctcg
     3181 tttccttccg gcagctcgcc cgcgtagcgg gggcgcaatg cgtcgcgacc ttggtagatc
     3241 gcatcgatct tcttcttcac gtcgccctcg agaccgttct tttttatgta tagctcgcac
     3301 tgttggtcta tggcggcgtt caggaagtaa gcgaggatca tgggtgcggg accgttgatg
     3361 gtcatcgaca ccgaggtctt gggatccgcg aggtcgaagc ccgaatagag cttcttcgcg
     3421 tcgtcgagac agcacaccga cacgcccgag ttgccgatct tgccgtagat gtcgggacgc
     3481 gtatcggggt cgcggccgta gagcgtcacc gagtcgaacg cggtcgacaa acgccgggcc
     3541 ggcatgtggt agctcacgta gtggaagcgg cggttggtgc gctcgggagc gccttcgccc
     3601 gcaaacatgc gggtcggatc ttcctcggtg cgtttgaagg gatagatgcc ggccgcgtac
     3661 gggaactcgc cgggcacgtt ctcggagagg ttccatcgca gcacgtcacc ccatgaacaa
     3721 tagcgcggta gcgctatctt tggaacctgg gtgtgcgaaa gactttcgga gtgtgtctgg
     3781 atcgagatgt ccttcccgcg gacttgaaac gtatagagag gctcgcggta acgcttcttc
     3841 ttctcgtccc atcccgcgat tatgtcctgg ttttccgggg ccaggtcgcg agctatttcc
     3901 gcatacgtcg attcgaggtc cttgacaatc cggtccttgt ccgccgcttt gccggcgcgt
     3961 accgtctcga tcgaagtccg cagggcaaat agcttctgcg cgacaccgga ctgttcattc
     4021 acccacttat cgtactcgcg gcacgtttcc gagatctcgg agaggtagcg cgtgcgcggc
     4081 ggcgggatga tgtaaatctt ctccgactgc tcggcggtga gatcgaagct cgagcgccac
     4141 ttctccgccg gcaggccgca cttggccacc agcgcgtcga ccaccgcgcg atagaggttg
     4201 ttggtcccgg ggtcgttgaa ctgcgacgcg atggtgccga acaccggtgt cttctcgtcg
     4261 tcgagatcga acttcttgtg cgcgcgcttg tactgtttgc gtacgtcgcg cagcgcgtcc
     4321 atggcacccc gcttgtcgaa cttgttgagc gcgatcaagt ccgcgaagtc gatcatgtct
     4381 attttttcga gttgggaggc ggcgccatac tcgggcgtca tcacgtacag gctcacgttc
     4441 gaatgatcga cgatctcggt gtcggactgc ccgatgcccg acgtctcgag caggatcaag
     4501 tcgaagttgg cggccttcaa gacgtcgatg gcgtctttca cgtgcgcgga gagcgccagg
     4561 ttcgactggc gggtggccag cgagcgcacg tacacgcgct tgctgcgaat cgcgttcatg
     4621 cggatgcggt cgccgaggag tgcgccgccc gtcttgcgct tggacggatc gacgcacacc
     4681 acgcccacgg tcttgtcggg gaaatcgatc aggaagcgcc gcaccagttc gtccaccaaa
     4741 gacgacttgc ccgcgccgcc ggtgccggta atgccgagga tcggcgcctt ggactccttc
     4801 gcgcgcgcgc gaaccttggc gaggtcgcgc tcgctctcct tgggcgcgtt ctcggccgcc
     4861 gagatggtct tggcgataga tccgaagtca cgcttcagga caccgtcgac cgcaccgttc
     4921 agcgacacgc cggtggcgaa gtcgctcttc atcacgaggt cgttgatcat cccctggagc
     4981 cccatcgcgc gcccgtcgtc gggcgaatag atgcgcgcga tgccgtactg gtgcagctcc
     5041 tcgatctcgt gggggaggat cacgccgccg ccgccgccga agatgcgaat gtgcgaggcg
     5101 ccgcgctgct cgagcagatc tttcatgtac ttgaagtact cgatgtggcc gccctggtag
     5161 gaggtcatcg cgatggcctg cgcgtcttct tgaatcgcgg cgtccaccac ttcttcgacg
     5221 ctgcggtcgt ggcccaaatg aatgacctcg cagccggtcg actggatgat gcgacgcata
     5281 acgttaatag ccgcgtcgtg gccgtcgaac agcgaggccg cggtgacgat gcgcaccttg
     5341 tgtttgggct ggtaggggtc gatgggtttc atgtcgaagg cccgcgccat gttgccgcgg
     5401 gctgggtgta tcttagcgaa tggcgtccgg atcggcaacg gccggtcgct agaagagcca
     5461 ctcgaatccg gcgtagaagc cgaacgcggc gtcctcgcgg gcgatcgact gctctggctc
     5521 cccggtgacg cccaggtcgc tctgcatccg gaagtgcagg aaaacggcgt tgcgttcctt
     5581 catgatcagt gccacgccgc cgtcgccgag ccagccgtag cgggagtaat cgcccgcgcc
     5641 gagctcctcg ggcgcgtcgg ggagggaact gggaccgccc aggaattcct gcgagatcag
     5701 gagtgcgtag tacaggcccc cgccgatcga tatccgaatc gtcgcgtcgt tcagcgaaac
     5761 cgtgttggtc ccgtgtaaga cgatcggcag ctccagaaaa tcttcgcgca taggctgcac
     5821 cgggaaaccg gtggggaaca acgccgacgt gtacgatccg tagaattcgc gcgacgcgta
     5881 gcgcgcctcg atgcgcacca tcgcgcgggt gaaggtccgc gcgtagaaaa gcccccaatc
     5941 gagggacgtg ctcgactcca tcgtctcgag atcggttccg gacgagacgt ggtgcgacgt
     6001 cggcgcggcg tagaaccccc acgagctcct gcgcgcctcc tgcgcggcgg cactcgccgc
     6061 gaacaacgtc aggacgatac cggtcacgac gatccgcgtc acttcgcgac ctcccgcgcg
     6121 agtcgttgga tctcgttcgc caggtctcct tcgaccggct cccggccata cccgtcgacc
     6181 cacatcaggc tttcgccatt ctcgcccacc cggcccaggt gccggacgaa gtgaatgggt
     6241 ctcggtggtg ccgccatcca cttcgctccg cgatcgaaga acatgtagat gtcccactcg
     6301 ggccggtcgc cgtactcacg cccgcgctcg agcaggacat cgcgcagcca gtggtcggcc
     6361 ggcagcgacg ccaacgcctc gtcgtacgcg tttgggaaca cctcgcgacg gtatgcgagg
     6421 ccgactcgcc gctccgggtc gtagaagtgt gcgacgtgct tcttgtcgaa catgcgcgcc
     6481 gcctcgcggg ccgcggcttc gtcgtctccc tccatcattg gcgcccacac gatggtgaca
     6541 tctattccct tgcgaccgac caccttctgc ttcacggcag aagctccgtg caggcactcc
     6601 gctcaggtgg gcgagaggat cgcgacgaac cgcaagcggt cggttcgcgc gttgaaatgc
     6661 gcgacgaagg gagcggtatc gtcagcgaga agaatgactt cgggggcgga gccgagagcc
     6721 gagactccga tcgcgaataa ggcgagggtt aaggtccgca cggccgtcag cataccatgc
     6781 gtatggcccc ggtgcgagcc gccgcgagcg cggcgatcgg gtggaaaggg tctggaatcg
     6841 gcaagcggcg gggtgtatcc tattgaccgt tcactagtgc cagccggtcc agcgtttccc
     6901 caacgttcca tggaggatgg cgaatgcgat accgactccc ctgtttcggt atttcccttc
     6961 tcgtactgcc cctgttcatc gtcggatcgg cgtttgcgtc gatgcgaccg gtcgccgatc
     7021 cggagccttc ggggggagcc acggctgcgg ccgaacagct ccgcgccggc ccgcgcgtcg
     7081 tgagcggcga tccggaggcc aatccgccgc tcggcgcgag cgccgacggc ttcaatttcg
     7141 acacgaacgg agtcctaacg ggcaatctgt tcatcccgcc ggatccccac tgcgcagcgg
     7201 gccccgacca cgtcgtcatg gtcgggaacg tcgccatcga gtggcggccg aaggacccgc
     7261 tagcggccgc accccaggtc ctcatgagtt tgcagtcgtt cttctcggcg ctgcccggcc
     7321 cgccgcccgc gccgggtacc acgctcggtt cgttcacgtt cgatccgaag gtgatctacg
     7381 atcagtatcg ccaacgtttc atcgcggtgg cactcgagcg ctgggacgtc gccttcggca
     7441 acccgtcgaa tcagtcgcgc attctggtcg ccgtgtccag gggaccggac ccgaacctcg
     7501 gttggtggtt ccactccatc gattcgaagc tgctcatcgg agccttcgag cactgggcgg
     7561 actatcccgg gctggcggtg ggagacgacg ccgtgtacat cacgaacaac atgttccgtt
     7621 tcgtggccgg cgggagtaat ttcggcggcg tgcgcctgtg gatcatcgac aaggtcccca
     7681 cctacgcggg tccgaacaac agcatcgcgt tcacggtgca cgatccctac gccgccgccg
     7741 gaatcgcgac gacaacgcag ccggcgcaca cgttcggccc gaccggcgcc ggttcgacgg
     7801 gcttccacct gggcacgttt ctattgagct attccggcat caccgacggc gtcaacgagg
     7861 cggtgcaagt ggtcgaggtg accgacccgc tgggcgccgt aggcggtccg ttcttcgttc
     7921 agcagttcgt gaacttcggc aacatcgaca acactgcggt ggtgacgctc ccggacgccc
     7981 cccaggcggg cggcgccccc gccatcgaag tgaacgaccg ccgggcgctc aacgccgttt
     8041 ggcgcagcaa caatatatgg acctgcgccg aactcgtccc cgtggccggt gcggacgtcg
     8101 gacaaacgac
//
LOCUS       DASPGK010000105         6240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_613772, whole genome shotgun sequence.
ACCESSION   DASPGK010000105 DASPGK010000000
VERSION     DASPGK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613772"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VEC56_02990"
     CDS             complement(<1..289)
                     /locus_tag="VEC56_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13149.1"
                     /translation="MDLTTACVKTLTSPRAFRAAALALGAAAIFVGQNAGAAKDVFPY
                     PATTRSLDNGLKVVLVPFDSPGIVAYYTIGRAGSRNEVEPGKSGFAHFFEHM"
     gene            590..715
                     /locus_tag="VEC56_02995"
     CDS             590..715
                     /locus_tag="VEC56_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13150.1"
                     /translation="MRYTILSNLGLIISVLAVFGKSIWGVMGNSTWGNSTWGGRG"
     gene            925..2631
                     /locus_tag="VEC56_03000"
     CDS             925..2631
                     /locus_tag="VEC56_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV13151.1"
                     /translation="MSTRKTPPPTPRKPAAPSTKKPAPRNRLEDFREWDRTLRASRPP
                     AASPTAAPAPPATPDAEAQRRFDELRKIVLLLGTAVDLKETLDRVVDGILVVARCERG
                     FVILMDAADHFATYTGRRKDQGEWDKDDARAISSTIVNAVIANGQPVIHSDLQEVDDL
                     RERGSIQAGKIRAAVCLPVVDDERLIGVIYADHRFVTPRYTQWDRSFLQFFALQAAFA
                     IGNARRKGELKHQGDRLAEENAALARQLFREFAMGGTISKSPAMLDVFEMVTKVAPDD
                     ITVLIQGESGTGKDGVARAIHARSRRREGPFCALSCGSIAANLVESTLFGYVRGAFTN
                     ADADRPGFFEAAQGGTLFLDEIGTMPLSAQVALLRVLENREFSRVGDVRVQRADVRVI
                     SATNRDLERAVAEGEFREDLYYRLNQAKIEVPALRERPEDIIPIAEHFLDQYAKNNKQ
                     MRPYLSGEAKAILLGHSWPGNVRELKSAIEGAILFRSADNSIDAKALERFISSRRGAP
                     SGESSPVTLRAVMERAEEQAVRQALAQHGNNVTLAAQALDLSRQQLYNKIRKYGIALR
                     LE"
     gene            complement(2635..3456)
                     /locus_tag="VEC56_03005"
     CDS             complement(2635..3456)
                     /locus_tag="VEC56_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HXV13152.1"
                     /translation="MFELLTYPFVQLALLAALILAGIHAYLGYHVVSRGVIFVDLSLA
                     QASALGATVAFCLGVRSEAVTYLVSLLFTLAGALVISVARTRDERVPHEAFIGILYAA
                     ATAVPLLLLSHHAEGAEMLHHMIAGSLLTVTPHELVKIAVLYAALGGFFFRYRKRFDT
                     ISRHRDRAQAEGWNLVWWDFLFYAAFALVVTSSVAIAGVLLVFALLVIPPVTALLMTT
                     HPAKRLALGWTIGVAGALVGVAASVAMDLPAGPAVIATLASILVVVAGLLRLARR"
     gene            complement(3459..4343)
                     /locus_tag="VEC56_03010"
     CDS             complement(3459..4343)
                     /locus_tag="VEC56_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXV13153.1"
                     /translation="MKRTLLLLLLIAVATNPVAAKVSVVASTTDLAAIATFVGGDAVE
                     VTSIARGTSDPHFVEVLPSYMVRVQKAKLYLKLGMDIDRWAAPIIDGSRNGKLVIVDC
                     SSRIAPLNVPTGKVDASMGDVHARGNPHYWLDPDNGVIIATTIADALSGIDPENTSAY
                     LANLERFKAELAARRTRWSDTAGGLRGMKMVTYHDSWPYFSRAFGVEIEDFVEPLPGI
                     EPTPSHTAKVIDMIKAQGIRVIGVEPYFSKRTPETIARATGAVVVELPPSVGGAPGAT
                     DYFSLFDVLIDRLMKAGR"
     gene            complement(4362..5372)
                     /locus_tag="VEC56_03015"
     CDS             complement(4362..5372)
                     /locus_tag="VEC56_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13154.1"
                     /translation="MSAIGDMRAVYRPSESAAGVGSNELTFDFEELELALNAYLNPYM
                     RADVFLSIHGTGPEVEIEEANFTVLRGLPAALQFNAGQYLLDFGKLNTQHPHQWAWLD
                     RPLMHRTMLGEDGLRALGARLTTLFAVGENALGLSASAFSGGAFESHEHGDEGEEDEA
                     PPDIMGSARASLFRQVSDAWSAELGASYLFGNYDPVEPLATQIAAVDWKLRWRPNSYQ
                     AFVWVAEAMSSEREVAEEIDSVTVNLSTVDAMGAFTSAELQFRKRWSAGAFFDWTQDA
                     SIQDAESTAYGAFFDFLPVEETARIGIVYRHETSDLTDFDDDSVTLQFLWSLGPHKVH
                     TF"
     gene            complement(5530..5808)
                     /locus_tag="VEC56_03020"
     CDS             complement(5530..5808)
                     /locus_tag="VEC56_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13155.1"
                     /translation="MKRVAALFLAAALVAAVVIHGAHGHDEDQHASRLHATCVVCQLG
                     SPVATQPVALPAVVDASPVCQLSIADRDRAIPPARVEVDLSRAPPLAS"
     gene            complement(5861..>6240)
                     /locus_tag="VEC56_03025"
     CDS             complement(5861..>6240)
                     /locus_tag="VEC56_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13156.1"
                     /translation="AAGLIAGGVSPGAAMVFLLAGPATNVSSFVVVKGLLGGRGLVGY
                     LASIVLVSVAAGVALDAFLDRLTLPTFAPSHAHESHSRVELVLTVVFLLLVAWSFART
                     RLLPRMWSRVRSYLAQAPRDSAR"
BASE COUNT         1168 a         2031 c         2065 g          976 t
ORIGIN      
        1 tcatgtgctc gaagaagtgc gcgaacccgg acttgcccgg ctcgacttcg ttgcgcgagc
       61 cggcgcgccc gatggtgtag taggcgacga tgcccgggct gtcgaagggc accagcacca
      121 ccttgagtcc gttgtcgagc gaccgggtgg tggcggggta agggaaaaca tccttggccg
      181 ccccggcgtt ttgacccaca aagatggctg cggcgcccag cgccagagcc gccgctcgaa
      241 aggcccgcgg cgacgtcagg gttttaacac aagcggtcgt caaatccatt tgacacctct
      301 aactgttttt gattcagctg gttaggatgc tcttgggttc ccgaaggggc gatgggtgtc
      361 aactctccct tacactcccc aaccgggatt gacccggacg cgcgcgcccc gagtggcgcc
      421 gtctggcaat aacgtgttgt agaaaggaag gttgcgaatc ttttgggaat ttctttccct
      481 tttggtacgg cggttgccat taccaggaac agaacggcca atgcagagcg aaggagacgg
      541 tttcgcggca gaggccccgg caaccaagtt ccaacgggag gtaaaagcca tgcgttacac
      601 gatcctttcg aatctcggac tcatcatcag cgtgctggcc gtgttcggca agagcatctg
      661 gggtgttatg ggcaatagca cctggggcaa cagcacctgg ggcggccgcg gctagttcac
      721 tgcttatatc ggattcccgc cgcgaatcca agcgggagtt cggtcggccg gaatagccgg
      781 ggagaagaga ctcgcgtggt ctctaatatc cgatgccggg gagaggccac gtggacgagc
      841 ccgcctgaaa aggcgggctc gctctatttg ggggagccgc gcgccctttc gcgaggggcg
      901 aggaaactga tacaatagaa ggcgatgtct acccgcaaga cacctccgcc gaccccacgg
      961 aagcccgccg cgcccagcac caagaagccc gcgccgcgca accgcctcga agactttcgc
     1021 gagtgggacc gcacactccg tgcatcgcga ccgccggccg cttcgcccac cgccgcgccg
     1081 gcgccgccgg ccacccctga cgccgaggcc cagcgtcggt tcgacgagct gcgcaagatc
     1141 gtcctgttgc tcggtaccgc cgtcgacctc aaggagaccc tcgatcgtgt ggtcgacggg
     1201 atcctcgtcg tggcgcgctg cgagaggggc ttcgtgatcc tcatggatgc cgccgaccac
     1261 ttcgccacct acaccggccg gcgcaaggac cagggcgagt gggacaagga cgacgcgcgc
     1321 gcgatcagca gcaccatcgt caatgccgtg atcgcgaacg gccagcccgt gatccattcc
     1381 gacctgcaag aggtcgacga cttgcgcgag cggggaagca ttcaggcggg gaagatccgc
     1441 gccgcggttt gcctgcccgt cgtcgacgac gagcgcctga tcggcgtcat ctatgccgac
     1501 catcgattcg tgacaccgcg gtacacgcag tgggaccgca gctttctgca gttcttcgcc
     1561 ctccaggccg cgttcgccat cggcaatgcg cgccggaaag gcgagctgaa gcaccagggc
     1621 gaccgcctcg cggaagagaa cgccgccctc gcgcgccaac tcttccgcga gttcgcgatg
     1681 ggcggaacga taagtaagag ccccgccatg ctggacgtgt tcgaaatggt gaccaaggtc
     1741 gcgcccgacg acatcaccgt gctgatccag ggcgaaagcg ggacgggcaa ggacggcgtc
     1801 gcgcgcgcga tccacgcccg cagccgccgc cgcgaggggc ccttctgcgc gcttagctgc
     1861 gggagtattg cggcgaatct ggtcgagagc acgctcttcg ggtacgtcag gggcgcgttc
     1921 accaacgccg atgcggaccg cccgggcttt ttcgaggccg cccagggtgg cacgctgttc
     1981 ctggacgaga tcggcaccat gcccttatcg gcacaggtcg cgctactgcg cgttctggaa
     2041 aaccgagagt tcagtcgcgt cggtgacgta cgcgtgcaac gcgccgacgt gcgcgtcatc
     2101 tcggccacca atcgggacct cgagcgagcg gtggccgagg gcgaattccg agaagatcta
     2161 tactatcggc tgaatcaggc caagatcgag gtgcccgcgc tgcgcgagcg ccccgaggat
     2221 atcattccga tcgccgagca tttcctcgat caatacgcga agaacaacaa gcagatgcgt
     2281 ccgtatctgt cgggtgaggc gaaggcgatt ctcctcgggc attcctggcc cggcaacgtg
     2341 cgcgaactca agagcgccat cgagggcgct attctgtttc gcagcgccga taactcgatc
     2401 gacgccaagg cgctggagcg tttcatctcg tctcggaggg gggcgccatc cggcgagtcg
     2461 tcgccggtga ccctgcgtgc agtgatggag cgtgcggagg agcaagcggt tcgtcaggcg
     2521 ctggcgcagc acggcaacaa cgtgaccctc gccgcccagg ccctcgactt gagccgccag
     2581 cagctctaca acaagatccg caagtacggc atcgccctgc gtctggaata gccgctagcg
     2641 ccgcgcgagg cgcagcaggc ccgccaccac caccaatatc gacgccagcg tcgcgatcac
     2701 cgccggcccc gccggcaggt ccatcgcgac cgacgcggcg acaccgacca gtgctccggc
     2761 cactccgatc gtccacccca gtgcgaggcg tttggccggg tgtgtcgtca tcaacaacgc
     2821 ggtgacgggc ggaatgacca gcagcgcgaa taccaggagc acgccggcga tcgcgaccga
     2881 cgaggtcacc accagtgcga aggcggcgta gaacaagaag tcccaccaca ccagattcca
     2941 tccctccgcc tgggcgcgat cgcggtgacg gctgatggtg tcgaagcgct tgcgatagcg
     3001 aaagaagaac ccgcccagag ccgcgtacaa caccgcgatc ttgaccagct cgtgcggcgt
     3061 caccgtgagc agcgaccccg cgatcatgtg gtgcagcatc tccgcgcctt cggcgtggtg
     3121 ggagagcaat agcagtggca ccgcggtggc agccgcgtac aagatgccga tgaacgcctc
     3181 gtgcggaact cgctcgtcgc gggtgcgcgc gaccgagatg acgagcgcgc ccgcgagcgt
     3241 gaagaggagc gagacgaggt aggtgaccgc ttcgctcctc acacccaggc agaaggcgac
     3301 cgttgcccct aacgccgacg cctgcgccag cgacaagtcg acgaagatga ccccgcggct
     3361 caccacgtga tagcccaagt acgcgtggat gccggccagg atcagcgcag ccaggagtgc
     3421 cagctgcacg aacgggtagg tcaggagctc gaacacgtct accgccccgc cttcatcaag
     3481 cggtcgatga gcacgtcgaa cagcgaaaag taatcggtcg cgccgggggc cccgcccacc
     3541 gagggtggga gctcgacgac gacggcgccg gtggcacgcg cgatcgtctc cggggtgcgc
     3601 ttggagaagt acggctcgac cccgatcacc cggatgccct gcgccttgat catgtcgatg
     3661 accttcgcgg tgtgcgacgg cgtgggctcg atcccgggca gcggctcgac gaagtcttcg
     3721 atctccacgc cgaacgcccg cgagaagtac ggccacgagt cgtggtacgt caccatcttc
     3781 atcccccgca atccgccggc cgtatcgctc cagcgggtcc tgcgggcggc cagctccgcc
     3841 ttgaatctct cgaggttggc aaggtacgcg gacgtgttct ccgggtcgat tccggaaagc
     3901 gcgtcggcga tggtggtcgc gatgattact ccgttgtcgg ggtccagcca gtagtgcggg
     3961 ttgccgcgcg cgtgtacgtc acccatcgac gcgtccacct taccggtggg cacgttgagc
     4021 ggtgcgatgc ggctcgagca atcgacgatg acgagcttgc cgttgcgcga cccgtcgatg
     4081 atgggtgcgg cccaccgatc gatgtccatt ccgagcttga gatagagctt cgccttctgc
     4141 acccgaacca tgtagctcgg cagtacctcg acgaagtgcg gatccgacgt gccgcgcgcg
     4201 atcgacgtca cctcgaccgc gtcgccgccc acgaaggtcg cgatcgcggc cagatcggtc
     4261 gtcgatgcca ccacgctgac cttcgccgcg acaggattcg tcgcgaccgc gatgagaagc
     4321 aagagcagca gtgtacgttt catgatggtg tctccgtagg actaaaaggt gtgcaccttg
     4381 tgcgggccca aagaccacaa gaactggagg gtcaccgagt cgtcgtcgaa gtcggtgagg
     4441 tccgaggtct cgtgccggta cacgatgccg atgcgcgcgg tctcttcgac cggcagaaag
     4501 tcgaagaacg cgccgtaggc ggtactttcc gcgtcctgga tggacgcgtc ctgcgtccaa
     4561 tcgaagaagg cccccgcgct ccagcgcttg cggaactgga gttccgcgga cgtgaacgcg
     4621 cccatcgcgt cgaccgtcga caggttcacc gtgaccgagt cgatttcttc cgcgacctcg
     4681 cgctcgctcg acatcgcctc cgcgacccac acgaacgcct ggtagctgtt cggcctccat
     4741 cggagtttcc aatcgacggc cgcgatctgc gtcgcaagcg gctcgacggg gtcgtagttg
     4801 ccgaagagat aggacgcgcc gagttcggcc gaccacgcat ccgagacctg ccggaacaag
     4861 gaggcgcggg cactgcccat gatgtcgggt ggcgcctcat cttcctcgcc ttcgtcgccg
     4921 tgctcatggg actcgaaggc acccccgctg aacgcgctgg cagacagccc cagcgcgttc
     4981 tctccgaccg cgaacagcgt ggtcaggcgc gcgcccagcg cgcgcaggcc gtcttctccc
     5041 agcatggtcc ggtgcatcaa cgggcgatcg agccaggccc actggtgcgg gtgctgggtg
     5101 ttgagctttc cgaagtcgag gagatactgg ccggcgttga actgcaacgc ggccggaagc
     5161 ccgcgcagca ccgtgaagtt cgcttcttcg atctcgactt cgggccccgt gccgtggatg
     5221 gagagaaaga cgtcggcccg catgtacggg ttgaggtacg cattgagcgc caactcgagc
     5281 tcctcgaagt cgaaggtgag ctcgttcgac cctacgccgg cggccgactc actcggccgg
     5341 tacacggcgc gcatgtctcc gatcgcgctc aagtccggat tggatagtgt ttgcgcccgc
     5401 gcgaacccgg tcgcgcatat cgccgcaagc cccgcgatcc aaaccgcgcg gggtcgcccg
     5461 ctggacaaca tagaaccctc ctcgcctgtg gagtgctgcg actctgtgta acgggaagaa
     5521 gaagtggagc taggacgcga ggggaggcgc gcgggagagg tcgacctcga cgcgcgcggg
     5581 tggaatggcg cggtcgcggt cggcgatgga aagttgacaa acgggggacg cgtcgacgac
     5641 ggcgggcagg gcgaccggtt gggttgcaac cggcgagccc aactggcaca ccacgcaggt
     5701 ggcgtgcagc cgcgacgcgt gctgatcctc gtcgtgcccg tgggcgccat ggatgaccac
     5761 cgcggcgacc aacgccgccg cgaggaagag tgcggccacc cgtttcatgc cccgaggatg
     5821 cgtcggctgg gagggaaagt caagccgcct tcgggcgggc tcagcgagcg ctgtcgcgtg
     5881 gtgcttgcgc gaggtacgag cgaacgcgcg accacatacg ggggagcagg cgcgtacgcg
     5941 cgaagctcca cgcgaccaag agcaagaaga ccacggtgag cacgagttcg acgcgcgagt
     6001 gcgactcgtg cgcgtgcgag ggcgcgaagg tcggcagcgt gaggcggtcc aagaacgcat
     6061 cgagtgcgac accggccgcc acgctcacga gcacgatcga cgccaggtag cccacgagac
     6121 cgcggccacc gagcaagccc ttcacgacga cgaagctcga cacattggtc gcgggacccg
     6181 cgagcaagaa gaccatggcg gcacccgggc tcacgccgcc ggcgatcagt cccgccgcga
//
LOCUS       DASPGK010000106         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_27552, whole genome shotgun sequence.
ACCESSION   DASPGK010000106 DASPGK010000000
VERSION     DASPGK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27552"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VEC56_03030"
     CDS             <1..1275
                     /locus_tag="VEC56_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HXV13157.1"
                     /translation="MFNEYIRQANFNQISPRSFWDNVEEGLHFDDNKFKTNQYVHPFN
                     GSTYFNSGRSNGLGFWASTLTAMTGATIWEVAGETHPASYNDLIATGIGGIAFGETVY
                     RLSSYLLDNTATGSGRTWREIGSFLIDPIHGFNRFVSGRASKVQGNPSDPYDWRPPRY
                     YTNLAIGARITGKGESINDSTEVRPVIDLYINHGNPFENARRKPFDHFDTGVQFNGDD
                     KNTLSRFQIRGDLFSKALGEAAYPKYAIAFTQYFDYVNNYDYEFGGQSFGGTFYSRFR
                     PSSNIGIQARVDLLWSILAAVNAEYSFLAQVEDRERFREYDYGPGGGVSAEAGVLHKG
                     RRVFWLGYRAQYVHVRNGSIWNPSETGSNAEHYVQSFGVKLAIPVRGAMNVGADASVF
                     RRDSDYEIAELQDTHNQRVPQARLYLSWETSR"
     gene            1402..1674
                     /locus_tag="VEC56_03035"
     CDS             1402..1674
                     /locus_tag="VEC56_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13158.1"
                     /translation="MQAIKKDLEYKRLQAVQMPFEEIETPGTYYSNWSGHLIRVPEDG
                     LKAGRSPVIDILGKSAMIVTKLSDDPFMTLTKARIVAADLDLEVNF"
     gene            1841..2458
                     /locus_tag="VEC56_03040"
     CDS             1841..2458
                     /locus_tag="VEC56_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXV13159.1"
                     /translation="MGVPVWITDPAGRISYLNTRAEALFGHTLSQWHGRPCHVAVAGR
                     TRTGPFCCPRCRVRSQAAAHAEIEPVHLEVRARDGSRTEVCVVVIAVEAPAGHHLVHM
                     VVDDERERRIRGFLERVVSRTAEDVGKPAPPAQRSAVSSTELSRREREVLSLLAADAN
                     LYEVAARLSISHVTVRNHVQHILSKLGVHSILEAVAVWLVDARRN"
     gene            complement(2464..>2717)
                     /locus_tag="VEC56_03045"
     CDS             complement(2464..>2717)
                     /locus_tag="VEC56_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13160.1"
                     /translation="PGAHDDYWRRGYTVALSYASQPVGDKEKAKEARFRVDLARANGR
                     LVWTGTSTPVDPKDAEKLRTEIAGQIVPELERRKLIPKD"
BASE COUNT          483 a          883 c          861 g          490 t
ORIGIN      
        1 atgttcaacg agtacattcg tcaggcgaac ttcaaccaga tcagcccgcg ctccttctgg
       61 gacaacgtcg aagaaggtct ccacttcgac gacaacaagt tcaagaccaa ccagtacgtc
      121 caccccttca acgggagtac atacttcaac tcgggccgct cgaacgggct cggcttctgg
      181 gcctcgacgc tgaccgccat gacgggcgcc accatttggg aggtggcggg cgagacgcac
      241 ccggcctcgt acaacgactt gatcgcgacc ggcatcggcg gcatcgcgtt cggcgagacc
      301 gtgtaccgcc tatcgtcgta tttgctcgac aacacggcga ccggcagcgg ccgcacgtgg
      361 cgcgagatcg ggagcttctt gatcgacccg atccacgggt tcaaccgctt cgtgtcgggc
      421 cgcgcgagca aggtgcaagg caatccgtcg gacccgtacg actggcggcc gccgcggtac
      481 tacacgaatc tcgccatcgg ggcgcgcatc accggcaagg gcgaatcgat caacgacagc
      541 accgaggtgc gtcccgtgat cgacctgtac atcaatcacg ggaacccgtt cgagaacgcg
      601 cgccgcaagc cctttgacca cttcgacacc ggtgtccagt tcaacgggga cgacaagaac
      661 acgctgtcgc gcttccagat tcgcggcgat ttgttctcga aggcgctggg cgaggcggcg
      721 tacccgaagt acgcgatcgc gttcacgcag tacttcgact acgtcaacaa ctacgactac
      781 gagtttgggg ggcagagctt cggggggacc ttctactcgc gcttccggcc ctcgtccaac
      841 atcggaatcc aggcgcgcgt cgatctgttg tggagcattc tggcggcggt caacgcggag
      901 tattcattcc tcgcccaggt cgaagaccgc gagcgcttcc gtgagtacga ctacggcccg
      961 ggtggcggtg tgtcggcgga agccggcgta ctgcacaagg gtcgccgcgt gttctggctg
     1021 gggtatcgcg cgcagtacgt ccacgtacgc aacgggtcga tctggaatcc gtccgagacg
     1081 ggctcgaacg cggagcacta cgtgcagtcg ttcggggtaa agctggccat tccggtgcgc
     1141 ggtgccatga acgtcggcgc ggatgcgtcg gtgttccggc gcgatagcga ctacgagatc
     1201 gccgagctcc aggacacgca caaccagcgc gtgccgcagg cgcgactgta cctgtcgtgg
     1261 gagacctcgc gctagtctcg ccagtgaccc ggcgaggatt cccgccccga aaatgatgca
     1321 agtgcatcat ggccgggccg ccggattgtc accatccttt ccaggtgagt actgatttct
     1381 ggaaaggagg aggtgaatcc gatgcaagcg atcaagaagg atctcgaata caagcggctt
     1441 caggcggtgc aaatgccgtt cgaagagatc gagacgcccg gcacctacta cagcaactgg
     1501 tccgggcacc tgattcgcgt ccctgaggat ggcctaaagg ccggacgctc gcctgtgatc
     1561 gatatcctgg gcaagtcagc catgatcgtc accaaactga gtgacgaccc attcatgacc
     1621 ctcaccaagg cccgtatcgt cgcggccgat ctcgacctcg aggtcaattt ctagacccct
     1681 ggttgggact tctgcaccaa acgcggcgcc ggattgctgc tattccacgc aaccggcgcc
     1741 gcgtggtatg atcgcaccgg ccgcccgttc catggccgaa atcgatcagg accgcatagc
     1801 cgcctctttt ggttccacgc cgccatcttg ggtggaatcg atgggcgtcc ccgtttggat
     1861 caccgaccct gccggtcgca tttcctattt gaacacccga gccgaggccc tcttcggcca
     1921 cacgctttcc cagtggcacg ggcgcccgtg ccacgtcgcg gtggcggggc gcacgcgcac
     1981 ggggccgttt tgctgccctc gctgtcgcgt gcgctcgcaa gcagccgccc acgcggagat
     2041 cgagccggtt catttggaag tcagggcgag ggatggatcg cgaacggaag tctgtgtggt
     2101 cgttatcgcg gtcgaggcgc ccgccgggca ccacctcgtc catatggtgg tcgacgacga
     2161 acgcgagcgc cggattcgcg gcttcctgga gcgggtcgtg agccggaccg cggaggatgt
     2221 ggggaagccg gcgccgccgg ctcagaggag cgcggtctcg tccacggagc tatcgcggcg
     2281 cgagcgcgag gttctctcgc tgctggccgc ggacgcgaac ctctatgaag tcgcggcgcg
     2341 cctctcgata agccacgtga cggttcgcaa ccacgtccag cacatcctct ccaagctcgg
     2401 cgtccactcc atcctggaag cggtcgcggt gtggctcgtg gacgcgcgcc ggaactgatc
     2461 gtctcagtcc ttgggaatca gcttgcgcct ctcgagctcg ggcacgatct gcccggcgat
     2521 ctcggtgcgc agcttctcgg cgtccttggg gtcgaccggt gtcgaggttc ccgtccacac
     2581 cagacgcccg tttgcgcgcg cgaggtcgac gcggaagcgc gcctccttcg ccttctcctt
     2641 gtctccgacc ggctggctcg cgtacgacaa cgccaccgtg tagccgcgcc gccagtagtc
     2701 gtcgtgggcg ccgggcg
//
LOCUS       DASPGK010000107         9801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_379016, whole genome shotgun sequence.
ACCESSION   DASPGK010000107 DASPGK010000000
VERSION     DASPGK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379016"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VEC56_03050"
     CDS             complement(<1..601)
                     /locus_tag="VEC56_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV13161.1"
                     /translation="MEKNYRHIETKLIHAGEPRLNGAVVMPIFQTAMFESKSDGDAGY
                     HDLRYIRLNNMPNHTVLAAKLAALENAQAALVTASGMAAITTSLLTVLKNGDHLLIQD
                     CTYGGTHSFVTHEFARYGIAYDVIDGADPGSWKAKLRSTTRAIYVEAIANPVMSVSDL
                     AAAADFAKEHGVVSMIDNTFASPMNFRPSEWGFDLSLHSC"
     gene            720..2432
                     /locus_tag="VEC56_03055"
     CDS             720..2432
                     /locus_tag="VEC56_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV13162.1"
                     /translation="MTISLRPHRFEAGNGRAVDAELGSLEVPMRRGAPAAGAVRLAFV
                     RLPATADRGRPPIVFLNGGPGLSGIRFGRGRYFGLFDGLRTAGDVILLDQRGSGSSTP
                     SLECAEPLALPFEQAFSREEVVRAVIESTRRCAEQLARSGIDIAAFNSAESAADVADL
                     ARALGAKQISLLGWSYGTHLAMAVLRRHDDLVARAVLAGPEGPDHTYKLPSRIERQLA
                     SIGERVRTAMPRAPDFVESVRDVLASVAREPARITLPVSGGTPRPAVIGRFDLEWMTA
                     EGIGDVRMIARMPRWYRAMARGDFGDIARDDLLRSHLEELRTGLNRSLVRACMDCASG
                     ASLERWRRIEDEARATLLGRTIDFPFPEVCDAIGRPDLGDAFRAPLHSRVPTLFVTGT
                     LDARTPADNVADLAPGFLNARHLVVEDAGHADLLLPAGVQRTIVRFFTDGDADTAHAR
                     AETPLRFEPATPVLLYDADCGFCRRRVEGLRAHVGDAVAFEPYQRAAWSGISTTDLAR
                     SVHFVDGNGDVSKGAEAILRARAAGRRRSAILWMYRRVPGFAPIAELVYDWVARRRGR
                     IGSI"
     gene            2630..3319
                     /locus_tag="VEC56_03060"
     CDS             2630..3319
                     /locus_tag="VEC56_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HXV13163.1"
                     /translation="MRTLSLLAAVLALSLAFTGCDDDDENNNPVAPPTPQASVNVTHA
                     SPDAPGVDLLVDDTPVGTNLTFPNSTGYLDVAAGTRNVKVNVTGTSTTAIEADLPLED
                     GKAYSVFAVNEVASIEAIVIEDNLAAPAAGKAHVRFLHASPDAPAVDVTTNTGAVVFG
                     DYVFKEYSPFTPLDAGTYDLEVRVAGTTTVALDLAPITLEAGKIYTVFAKGLLAGAGG
                     QALGAQIIVNN"
     gene            complement(3443..3850)
                     /locus_tag="VEC56_03065"
     CDS             complement(3443..3850)
                     /locus_tag="VEC56_03065"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HXV13164.1"
                     /translation="MPIFGWIEPEVVAIFIPIIGVLGGVLIAVTAVVVGGRRKELEHR
                     ERIIAMEKGLPVPGPMVEPERPKYAGRRANGLVMTGIGLALTIALSAEEGFADGGVWG
                     LIPLFIGVGLLVAGSLDKREWETQQRKNNSTPM"
     gene            4063..4638
                     /locus_tag="VEC56_03070"
     CDS             4063..4638
                     /locus_tag="VEC56_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV13165.1"
                     /translation="MSNDQDRELVARSLRGDGRAFRDLVGKHHATAYAVVRAILGPQR
                     FDDVDDVMQLTYLKAYQGLGSFRGESKFSTWLYQIARREALDVVSKKRVDTTSIDDVE
                     IPARADATPDAATHERGERERIERAMAELDESYRTAIELRYMAEKSYEEIAELMRLPV
                     GTVKTYVHRGKLELKKILSRPAWRERKDAVS"
     gene            4638..5171
                     /locus_tag="VEC56_03075"
     CDS             4638..5171
                     /locus_tag="VEC56_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV13166.1"
                     /translation="MNRFDPMDCERIEAKLPLFVARELSADEAASVSTHLDACPQCRD
                     ALASFVSIEQALVARRDEVPAIDAFLPDMSAAGFRARRGILIRSFRALMSVPGVAIVL
                     VMWGALLFGRYSDRLTGEATQKTMLERLTAVAKHGLDAMVAAAGGDAWTLTAVYGALA
                     IGVIVSAGALTVRFVRS"
     gene            complement(5092..5817)
                     /locus_tag="VEC56_03080"
     CDS             complement(5092..5817)
                     /locus_tag="VEC56_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXV13167.1"
                     /translation="MTKRPTQRTRVRRLPKRGAYDRATIDAILDEGLVCHVGFVVDGQ
                     PFVIPTLHARDGDRVLIHGSAASRMLRNLGAGVDACLTVTLADQLVIARSAFHSSMNY
                     RSVMLLGKARLIEEPRAKMDALRALVEHLIPGRWDDCRRPNAKEAKATWILELPIDEA
                     SAKIRTGPPIDDDEDYALDIWAGLLPLAKGFGKPIRDAKLKRGVEAPSYLTRYARPSS
                     ARSAPSKPPRKRSRRSRAPRTPR"
     gene            complement(5814..6596)
                     /gene="nth"
                     /locus_tag="VEC56_03085"
     CDS             complement(5814..6596)
                     /gene="nth"
                     /locus_tag="VEC56_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXV13168.1"
                     /translation="MKRVTARSQRRKAPRVPKHVLARKGPPKQGPPPRPKKKVTAKRK
                     PLPVVATPELRRRALRLHDGLKDAYPDARCELDFTNALEALVSTILAAQCTDARVNMV
                     TPALFKKYPTARDYLNADDDVLKEEIKSTGFFNNKTKAIKAAAKLLVEEFSGEMPRTM
                     EDLLKLPGVGRKTANVILANIYGVPGVVVDTHMLRLSNRMGFSVQHDADKVEKDMMAI
                     FPESTWISLSHLIPWHGRRCCTARNPNCDGCPVADECPKLIE"
     gene            6691..7056
                     /locus_tag="VEC56_03090"
     CDS             6691..7056
                     /locus_tag="VEC56_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV13169.1"
                     /translation="MTYQPKLVALADPTRRAILERLRHGAMPVGKLAAGFDVTRPAVS
                     QHLKILQDAGLVRSQRVGTSRFYSVDAAGLDELRRYLDHFWADVLAAFRNETERVTAP
                     AKKPGRTIRQRKAKKHGKR"
     gene            7043..7510
                     /locus_tag="VEC56_03095"
     CDS             7043..7510
                     /locus_tag="VEC56_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV13170.1"
                     /translation="MANAERAREPIAPLRKSIVVGRTPAEAFEIFTARFGTWWPFQKF
                     SVNQAKARGCAMETRLGGAIYEIAPDGKRVAWGHVIAWEPPHRFVMSWYPGRDPETGQ
                     EVELRFIAVKEGTRVELEHRDWEKLGDIATEERRNYENGWAVVFNQRFAEACR"
     gene            complement(7553..8263)
                     /locus_tag="VEC56_03100"
     CDS             complement(7553..8263)
                     /locus_tag="VEC56_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXV13171.1"
                     /translation="MSTDRPDRTESPYTVPPGWFQIESDLATAGRIAGDDERLTETAL
                     LTINAKYGVTHRIDLQFVFNPWERVEATQEGFPDQSDSGTGSAGLRAKFNLFGNDDGG
                     IAFALLPFATVPTRGDAILDFITWGMVAPLAIPIGDAAALTFMGGITSIDDEDWWGSV
                     SGSFGTAIAGDFAGFVEFYLSRSSFDSDAIDDTTFDIGVTFAPGDNWQLDAGVYRGLA
                     TETEDWRVFLGASVRTPL"
     gene            complement(8442..>9801)
                     /locus_tag="VEC56_03105"
     CDS             complement(8442..>9801)
                     /locus_tag="VEC56_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXV13172.1"
                     /translation="AGRDGKLAVLPGFGEKTQLNILNAVARYRKYAQRFLFSVAEDEA
                     NGILEAVRRVKGVERTLIGGSLRRHRETIGDIDILATAEDADPVMEVFTTLANVATVI
                     AKGPTKSSVIMKSGIHVDLRVVKDDEFSAASHYFTGNKEHNTEVRARAKKLGFKLNEY
                     GLFEGEHAVKIMDEEALFAKLGMDFIPPELRENAGEIEAAEEHTLPKLIEVGDIRGTF
                     HCHTTYSDGRSTLREMAEAAKAMGLEYLGIADHSQTAVYAGGMDKAKVKKQKREIEKL
                     NEELAPFVLFAGIESDILAEGQLDYDDETLSLFDHVVASVHGQFTGTEAQMTKRIVTA
                     VSNPLTTMLGHPTGRLLLSRDGYPLNLTAVFEACAAHKVMIEINAHPYRLDLDWRHLR
                     TAKDMGVHFVINPDAHHTSEIGYFRYGVGVARKGWLTKGDVFNTRNAAEVRRRFEARR
                     KG"
BASE COUNT         1624 a         3161 c         3258 g         1758 t
ORIGIN      
        1 tgcagctgtg cagcgacagg tcgaagcccc actcggaggg acggaagttc atcgggctcg
       61 cgaacgtatt gtcgatcatc gagaccacac cgtgctcttt ggcaaagtcg gccgcggccg
      121 cgaggtcgct gacgctcatg accgggttgg cgatggcttc gacgtagatg gcgcgcgtgg
      181 tcgagcgcag cttggctttc cacgaaccag ggtcggcacc gtcgatgacg tcgtaggcga
      241 taccgtagcg cgcgaactcg tgggtcacga acgagtgcgt gccgccgtag gtgcaatcct
      301 ggatcaacaa gtggtcgccg ttcttgagca cggtgagcag cgacgtggtg atggcggcca
      361 tgccgctcgc ggtcaccagc gccgcctgcg cgttttcgag cgccgcgagc ttggccgcga
      421 gcacggtgtg attcggcata ttattgagcc gaatgtagcg caggtcgtgg tatcccgcgt
      481 cgccgtcgct cttcgactcg aacatggcgg tttgaaagat gggcatgacg acggcgccgt
      541 tcaggcgcgg ctcgccggcg tggatgagct tggtctcgat gtgccgatag ttcttttcca
      601 ttggatggct ccttggcagt gcgcgtgtcc gcccggagac gcgccggcgg acttctctcg
      661 tgagcgcgga aagctataca atacggggcc cgcgccacgt tgacaagcgc gcgcatcgca
      721 tgacgatttc gctccgaccg catcgattcg aggccggcaa cggccgcgcc gtggacgccg
      781 agctcggctc cctcgaggtc cccatgcgcc gcggcgcacc cgccgcgggc gcggtccggc
      841 tcgcgttcgt gcgactcccc gccaccgcgg accgcgggcg cccgcccatc gtgttcttga
      901 acgggggacc ggggctctcg gggattcgct tcgggcgcgg gcgttacttc gggctgttcg
      961 acggtctgcg cacggcgggg gacgtgatcc tgctcgacca gcgcgggtcg ggatcctcga
     1021 ccccgtcgct cgagtgcgcc gagccgctgg cactcccgtt cgagcaggcg ttctcgcgcg
     1081 aggaggtggt gcgcgcggta atcgagtcga cgcgccgctg cgctgagcag ctcgcgcgta
     1141 gtgggatcga catcgccgcg ttcaacagcg cggagagcgc ggccgacgtg gccgatctcg
     1201 cgcgggcgct gggtgccaag cagatctctt tgctcggttg gagctacgga acccacctcg
     1261 cgatggcggt actgcgccgg catgacgacc tggtggcgcg cgccgtgctg gccggacccg
     1321 aaggacccga tcacacctac aagctgccgt cgcgcatcga gcgccagctc gcatcgatcg
     1381 gcgagcgcgt gcgcaccgcg atgccgcgcg cgcccgactt cgtcgagagc gtgagggacg
     1441 tgctggcgtc ggtagcgcgt gagcccgcgc ggatcacgct gcccgtttcc ggcggcacgc
     1501 ctcgcccggc cgtgatcggc cgcttcgacc tggagtggat gacggccgaa ggaatcgggg
     1561 acgttcgcat gatcgcacgc atgccgcgct ggtatcgcgc catggcgcgg ggcgacttcg
     1621 gcgacatcgc gcgcgacgac ctgttgcgct cccacttgga agagcttcgc acggggctca
     1681 atcggtcgct cgtgcgcgcg tgtatggact gcgcgtcagg tgcctcgctc gagcggtggc
     1741 gccgcatcga ggacgaggcg cgcgcgacgc tcttggggcg gacgatcgac tttccgttcc
     1801 cggaggtctg cgacgcaatc ggacggcccg acctcggcga cgcgttccgc gcgccgctgc
     1861 actcgcgtgt gccgacgctc ttcgtcacgg gtacgctcga cgcgcgcact cccgccgaca
     1921 acgtcgccga tctcgcgccc gggttcttga acgcacgcca cctggtcgtc gaggacgccg
     1981 ggcacgccga cctgctgctg ccggccgggg tgcagcggac aatcgtgcgc ttcttcacgg
     2041 acggagacgc cgataccgcg catgcgcgcg cggagacccc gcttcgcttc gagcccgcca
     2101 cgccggtgct cctctacgac gccgattgtg gattctgtcg ccgccgcgtc gaagggctac
     2161 gcgcgcacgt cggcgacgcg gtggcgttcg agccgtatca acgggcggcg tggtccggaa
     2221 tctcgacgac cgaccttgcg cgctcggtgc acttcgtcga cgggaacggg gatgtgtcga
     2281 agggggcgga ggccatcctc cgagcgcgcg ccgcgggccg gcgcaggtcg gcgatcctat
     2341 ggatgtatcg acgcgtcccg ggcttcgccc cgatcgccga gctcgtctac gactgggtcg
     2401 cccgccgccg cgggcgtatc ggctctatct agataatttt attagagtta ctccaacgta
     2461 gcgttgcgga aagccgaggc ggggaaacgc ctgtccatat tttgtctatg atttgtccaa
     2521 aaatatttgc gcgcgcgtca aggttttccg accttgttgg ccgagcccca agccggtcgg
     2581 acgaccggcg gcaaaacgaa ctcgaaccag gtccacagga ggacacgcaa tgcgcactct
     2641 ctccctgctc gcggccgtgc tggcgctctc gctggcgttc accggctgcg acgacgacga
     2701 tgagaacaac aaccccgtcg cgcctccgac gccgcaggcg tcggtcaacg tgacgcacgc
     2761 ctcgcccgac gcgcccggcg tcgacctctt ggtcgacgac acgcccgtgg ggacgaacct
     2821 cacgtttccg aacagcaccg gctacctcga cgtcgcggcc ggcacgcgca acgtgaaggt
     2881 caacgttacc ggcacgtcga cgacggcgat cgaagccgat ctgccgctcg aagatggcaa
     2941 ggcctattcg gtgttcgcgg tcaacgaagt cgccagcatc gaggctatcg tgatcgagga
     3001 caacctcgcc gcgcccgcgg ccggaaaggc gcacgtgcgc ttcctgcacg cctcgccgga
     3061 tgcgcccgcc gtcgacgtca cgaccaacac cggcgccgtc gtcttcggcg actacgtgtt
     3121 caaggagtac tcgccgttca cgccgctgga cgcgggcacc tacgacctgg aagttcgcgt
     3181 ggccgggacc accaccgtcg cgctggactt ggctccgatc acgctcgaag cgggtaagat
     3241 ctacacggtg ttcgcgaagg gcttattggc cggcgcgggc ggccaggcct tgggcgcgca
     3301 gatcatcgtc aacaactagt catcgcaagc agtcgaaaaa gcgaatcgcc gctcgcgcgt
     3361 tgctgcgcgg gcggcgattc acgttggggg cgctgactcg cgggtgtccg tttgcgacgc
     3421 gcccccgcat gcgtcgttcg cactacatcg gcgtgctgtt gttcttccgc tgttgggttt
     3481 cccattcgcg cttgtccagc gaaccggcga cgagcaaccc gactccgatg aagagcggga
     3541 tcaatcccca cacgccgccg tccgcgaaac cttcctcggc ggacaacgcg atggtcaatg
     3601 ccaagccgat cccggtcatc accagaccgt tcgcgcggcg acccgcgtac ttggggcgct
     3661 cgggctcgac catcggtccc gggaccggaa ggcccttctc catcgctatg atgcgttcgc
     3721 gatgttcgag ctctttgcga cgaccgccca cgaccacggc ggtcaccgcg atcaatacgc
     3781 cacccaagac cccgatgatg gggatgaaaa tggcgaccac ttcaggttcg atccagccga
     3841 agattggcat gacaggttcc tttcgtgtcc ggcgaataag tgtctatttt gcatggggtt
     3901 gggctacctt cgactgggcc cgactccgcc attcactccc ttggaccgtc cccgggccgg
     3961 ccgcgtttca agaagttttc ggcggggcgg tcctcgcggg tccgctaaga tagatgaaac
     4021 ggccgggcct ggggcgcggt cgtagcatgc ggagacgatg atttgtcgaa cgaccaggat
     4081 cgcgagctcg tcgcacgatc gctccgcgga gacgggcggg cgtttcgcga cctcgtcggc
     4141 aagcaccacg ctaccgccta cgcggtggtg cgcgccatcc tggggccgca acgattcgat
     4201 gacgtggacg atgtcatgca gttgacatac ctcaaggcat accaagggct cgggtccttt
     4261 cgcggcgagt cgaagttctc gacctggctc taccagatcg cgcgccgcga agcgctcgac
     4321 gtggtttcga agaagcgcgt ggacaccacc tctatagacg acgtcgagat ccccgcccgc
     4381 gccgatgcaa ccccggatgc cgcgacgcac gagcgcggcg agcgcgaacg aatcgagcgc
     4441 gcgatggccg aattggacga gtcctatcgc accgccatcg agcttcggta tatggccgag
     4501 aaatcctacg aagaaatcgc cgagttgatg cgcttgccgg tgggcacggt caagacgtat
     4561 gttcatcgtg gcaagctgga attgaagaag atactgtcgc gcccggcgtg gcgggaacgc
     4621 aaggatgccg tgagttgatg aatcggttcg accccatgga ctgcgaacgc atcgaggcga
     4681 agctgccgct cttcgtcgcg cgcgagcttt ccgccgacga ggcggcgtcc gtgtcgactc
     4741 acttggacgc gtgcccgcag tgccgcgacg cgctggcttc tttcgtgtcg atcgagcagg
     4801 cgctggtcgc gcgccgcgac gaagtgcccg cgatcgacgc cttcctgccc gatatgagcg
     4861 cggctggttt tcgcgctcgc cgcggcattc tgattcgaag cttccgcgcg ctgatgagcg
     4921 tgcccggagt cgcgatcgtg ctcgtgatgt ggggggcact cctgttcggg cgctacagcg
     4981 atcgcctgac gggcgaggcg acgcaaaaga cgatgctcga gcgcttgacc gcggtcgcca
     5041 agcacggtct cgacgcgatg gtcgcggccg ccggcggcga cgcgtggacg ctcaccgcgg
     5101 tgtacggggc gctcgcgatc ggcgtgatcg tttccgcggg ggctttgacg gtgcgcttcg
     5161 tgcggagcta ggccgcgcgt agcgcgtgag gtaggacggc gcttcgaccc cgcgtttgag
     5221 cttcgcatcc cgaatcggct tcccgaatcc cttcgccagc ggaagcaaac ccgcccagat
     5281 atccagcgcg tagtcttcgt cgtcgtcgat cggcggtccc gtgcgaatct tggccgacgc
     5341 ttcgtcgatc ggcagctcga gaatccaggt cgccttcgcc tctttcgcat tgggacgccg
     5401 gcagtcgtcc cagcgtcccg ggatcaagtg ctcgaccagc gcgcgcagtg cgtccatctt
     5461 ggcgcgcggc tcttcgatca agcgcgcctt tcccaacagc atcaccgacc ggtagttcat
     5521 cgagctgtgg aacgccgagc gcgcgataac caactggtcg gccagcgtca cggtgaggca
     5581 cgcgtcgacg cccgcgccga ggttgcgcag catgcggctc gcggccgagc cgtggatcag
     5641 cacgcgatcg ccgtcgcgcg cgtggagggt ggggatcacg aagggctgcc cgtcgacgac
     5701 gaagccgacg tggcatacca gaccctcgtc caggatggcg tcgatggtgg cgcggtcgta
     5761 ggcgccgcgc ttggggaggc gacgcacgcg ggtgcgttgg gtaggccgct tggtcattca
     5821 atcagcttcg ggcactcgtc ggcgaccggg cagccgtcgc agttgggatt gcgcgcggtg
     5881 cagcagcggc gtccgtgcca cggaataaga tgagagagcg agatccatgt gctctcgggg
     5941 aagatcgcca tcatgtcctt ttcgactttg tcggcgtcgt gctggaccga gaagcccatg
     6001 cggttcgata accgcagcat gtgggtatcg acgaccacgc cggggacgcc gtagatgttg
     6061 gccaggatca cgttcgcggt cttgcggccg acaccgggga gcttgagtag gtcttccatg
     6121 gtgcgcggca tctcgccgct gaactcctcg acgagcagct tcgcggccgc cttgatggcc
     6181 ttggtcttgt tgttgaagaa gccggtgctc ttgatctctt ccttcaagac gtcgtcgtcg
     6241 gcgttgagat agtcgcgcgc ggtcggatac ttcttgaaca gcgcgggcgt gaccatgttg
     6301 acgcgcgcgt cggtgcactg cgcggccagg atggtcgaga ccagcgcctc gagcgcgttg
     6361 gtgaagtcga gctcgcagcg cgcgtcgggg taggcgtcct tcaatccatc gtgcagccgg
     6421 agcgcgcggc ggcgcagctc gggtgttgcg acgacgggaa gaggcttccg cttcgcggtg
     6481 actttcttct tcggccgggg gggagggccc tgcttcggag ggcctttccg ggccaggacg
     6541 tgcttgggca ctcggggtgc cttgcggcgc tgcgatcgtg cggtgacgcg tttcatggcg
     6601 tgatcataca aaggggtgct cggggtcgcg tccatgttcg ccgcaaaatc gcgtcttgac
     6661 aagtaggtaa gtatccacta accttaagcc atgacttacc aacccaagct cgtcgccctg
     6721 gccgatccca cccgccgcgc tattctggaa cgtctgcgac acggcgcaat gccggtcggc
     6781 aagctcgcgg ccggcttcga cgtgacgcgg cccgcggtgt cgcagcatct caagatattg
     6841 caggacgcgg ggctggttcg gtcgcagcgc gtggggacga gtcgcttcta ctccgtggac
     6901 gcggccggac tcgacgagct gcgccgctac ctcgaccatt tttgggccga cgtgctggcc
     6961 gcattccgca acgagaccga acgcgtgacc gcgccggcca agaaaccggg ccgcaccatc
     7021 cgccagagaa aggcgaaaaa acatggcaaa cgctgaacgc gcgcgcgaac cgatcgcgcc
     7081 gctgcgcaag tcgatcgtcg tgggccgcac gcccgccgag gcattcgaga ttttcacggc
     7141 ccgcttcggc acgtggtggc cgtttcaaaa attctcggtg aaccaggcca aggcgcgcgg
     7201 gtgcgcgatg gagacgcgcc tgggcggcgc gatctacgag atcgcgcccg acggcaagcg
     7261 cgtcgcgtgg gggcatgtga ttgcgtggga gccgccgcac cgattcgtga tgtcgtggta
     7321 tcccgggcgc gatcccgaga cggggcaaga agtcgaactg cgcttcatcg ccgtgaaaga
     7381 agggacgcgc gtcgagctcg agcatcgcga ctgggagaaa ctgggcgaca tcgccacaga
     7441 agagagaaga aactacgaaa acggctgggc cgtcgtattc aaccagcgct tcgcggaggc
     7501 gtgccgatga tgcccatgcc accgaccatc aaggtagcac tggccggatg agctagaggg
     7561 gtgtgcgcac gctcgcgccg agaaagacgc gccagtcttc ggtctcggtg gcgagtccgc
     7621 ggtagacgcc ggcgtcgagt tgccaattgt cgccgggcgc gaaggtgacg ccgatgtcga
     7681 aggtagtatc gtcgatggcg tcggagtcga agctgctgcg cgagaggtag aactccacga
     7741 accccgcgaa gtctcccgcg atcgcggtcc cgaaggagcc cgacaccgac ccccaccaat
     7801 cctcgtcgtc gatgctggtg atacccccca tgaacgtcag cgcggcggcg tctccgatgg
     7861 gaatcgcgag cggcgccacc atcccccacg tgatgaagtc gaggatcgca tccccgcgcg
     7921 tcggcacggt cgcgaagggg agcagcgcga acgcaattcc gccgtcatca ttaccgaaca
     7981 agttgaactt cgcgcgcagg ccggcgctcc cggtgccact gtcgctctga tcgggaaatc
     8041 cctcctgcgt cgcctccacg cgctcccacg gattgaacac gaactgcaga tcgatgcgat
     8101 gggtgacgcc gtacttcgcg ttgatcgtga gtaacgcggt ctccgtcagt cgctcgtcgt
     8161 caccggctat gcggcccgcg gtggcgagat ccgactcgat ctgaaaccag cccggcggca
     8221 ccgtgtacgg actctcggtg cgatcggggc ggtcggtgct caacggacgc tcttgtgcga
     8281 acgcgattgc aggagacacg ctcgcgacag cgagcgaggc aagaagtcca cgcaatagag
     8341 ccatgctgca actatatggg acgtgcgcat caaagtgaac ggagaagacg cgccgcacgc
     8401 tcgattagga agagactaag tcgtagtcga ggactaaaac gtcagccctt gcgacgtgcc
     8461 tcgaaccggc gccgcacttc ggcggcgttc ctcgtgttga agacgtcgcc cttggtgagc
     8521 cagcccttgc gcgccacacc cacgccatag cggaagtagc cgatctcgct cgtatggtgt
     8581 gcgtcggggt tgataacgaa gtggacgccc atgtctttcg cggtacgaag gtggcgccaa
     8641 tccaggtcga ggcggtaggg gtgcgcattg atctcgatca tgaccttgtg tgccgcgcac
     8701 gcctcgaaca cggcggtcag attcagcgga tatccgtcac gagaaagcag caagcgccca
     8761 gtcgggtggc ccagcatggt ggtgagggga ttcgacaccg cggtcacgat gcgcttggtc
     8821 atctgggcct cggtaccggt gaactgcccg tgcaccgatg ccaccacgtg atcaaagagt
     8881 gagagcgtct cgtcgtcgta gtcgagctgt ccttcggcca agatatcgct ctcgatgccc
     8941 gcgaataaga cgaacggcgc caactcttcg ttgagcttct cgatctcgcg cttctgcttc
     9001 ttgaccttgg ccttgtccat tccacccgcg tataccgcgg tttgactatg atccgcgatg
     9061 ccgaggtact cgagacccat cgccttggcc gcctccgcca tctcacgcaa tgtcgagcgc
     9121 ccgtcgctgt aagtggtgtg acaatggaac gtgccgcgaa tgtcgcccac ttcgatgagc
     9181 ttggggagcg tgtgttcctc ggccgcctcg atctcgcccg cgttctcgcg cagctcgggc
     9241 gggatgaagt ccatcccgag cttcgcgaac agcgcctctt cgtccatgat cttcaccgcg
     9301 tgctcgccct cgaagaggcc gtactcattg agcttgaagc ccagcttctt cgcgcgcgcg
     9361 cgcacttcgg tattgtgctc cttgttgccg gtgaagtagt gcgaggcggc cgagaactcg
     9421 tcgtctttga ccacccgcaa gtcgacatgg atgcccgact tcatgatgac gctcgacttg
     9481 gtgggcccct tcgcgatcac ggtggcgacg ttggcaagcg tcgtgaacac ctccatcacc
     9541 gggtcggcgt cctctgcggt ggcgagaatg tcgatgtcgc cgatggtctc gcgatgccgg
     9601 cgcaggctcc cgccgatcag cgtgcgctcc acacccttca cgcgccgcac cgcctcgagg
     9661 atcccgttgg cctcgtcttc ggcaacacta aagaggaaac gttgcgcgta ttttcggtag
     9721 cgcgcgaccg cgttcaagat gttgagctgc gtcttctccc cgaagcccgg cagcaccgcc
     9781 agcttcccgt cgcgccccgc c
//
LOCUS       DASPGK010000108         6799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_964621, whole genome shotgun sequence.
ACCESSION   DASPGK010000108 DASPGK010000000
VERSION     DASPGK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964621"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VEC56_03110"
     CDS             <1..840
                     /locus_tag="VEC56_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXV13173.1"
                     /translation="EHLDFYPDLAAIEEAFVEYANRVPFYGAVILCADDARTMEIAPR
                     IRRRTVTYSLGGRADIQGSVVERGAGWTRFAVSDARGPLGDVTLHIPGDHYARNALAA
                     VAAGRWLDIPFEQIRAGLESWQGVGRRFEVKGEAGGVLVIDDYGHHPTEIAATIQAAL
                     SSYRRRLFVLFQPHRYSRTQALAAEFGTCFDGAHRVFLTDIYAAGEKPIAGVSTDRIV
                     ERVKRDGRTEVEHVRTYEDGVRRIAQDVQRGDMVITMGAGDVTKAGELLLSQLGAPAG
                     RKG"
     gene            844..1758
                     /gene="murB"
                     /locus_tag="VEC56_03115"
     CDS             844..1758
                     /gene="murB"
                     /locus_tag="VEC56_03115"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXV13174.1"
                     /translation="MTSAIDWPGAAVRLSAFLAGRVRVDEPMSKHTTIGVGGPARVMV
                     FPRTPAEVGRVVRLALALGLPYLPVGKGSNLIVRDGGYDGVVIQLAEHMTRVRVDART
                     VWAEGGASFAALARKMTKAGRTGLEFGIGIPGSVGGAVRMNAGAFGGEVKDALARIRV
                     VDEAGRLRAMAASQIVFSYRHTSIPARAVVVEAVFRCAPGTIRKDVYERSIGRKETQP
                     IWERSFGSTFVNPPGRYAAEMIEGCGLKGTRLGGAMFSDLHANFIVNVEGRASAADVE
                     ALIATAQREVKKKYGVDLKTEVIVIGNR"
     gene            1745..2515
                     /locus_tag="VEC56_03120"
     CDS             1745..2515
                     /locus_tag="VEC56_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXV13175.1"
                     /translation="MGIVSTEAERKRTLRARARRVIAVFIVGVLIVASLYLYDYVTTA
                     DRFAVEHVELSGLSRIDRAEVERLVADLKGHNILLAPLDVYEARLEAHPRIASVAVRR
                     VLPDRVVYVVTEREPVALVFTDRYYEIDADGVVMPGDAYTGMLDLPTITGVSARDVRA
                     GRVCDQPLVRGALEALSVCRSIGRGFAEDISEVRASEIGLSIRSLKDDCVLLVGDGDF
                     EKRLRKFFLLRDELARRDSASGLIDLRFDNQVVLRSDI"
     gene            2576..3814
                     /gene="ftsA"
                     /locus_tag="VEC56_03125"
     CDS             2576..3814
                     /gene="ftsA"
                     /locus_tag="VEC56_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXV13176.1"
                     /translation="MIMKSARKLYGAIDLGTTKVAALVAEEAGGALRVVGAGLAPSEG
                     MRQGVVVDIERASVCVARAVAEAERMAGATPRTYNVGAAGEHVRSMNSRGVVSVGDIE
                     KEIAPEDVARAFEAARKFSLPQDREIIHTLPQEFIVDSQRGIRQPAGMFGARLEARVH
                     VVTASRPALDNLAKTLLLAGVEVGDIVLEPLASATAVLSADEREHGAIVLDVGGGTTD
                     VLVVADGGVLASGVIGLGGHNITSDVAYGLRTSPREAERLKVEHGSALSTLVAGDEVV
                     QVSMVAGRGPRRVAKTVLSGIIEPRVSELFGLIDQQIAQSGLKRSLRAGVVLTGGSAM
                     LTGIRELAEQVFDLPARVASPTGLEGLSEVVAHPRFSTAVGLLRALAPAVMPGERASE
                     RGGRLASGLYSFKRAIASFI"
     gene            3830..5266
                     /gene="ftsZ"
                     /locus_tag="VEC56_03130"
     CDS             3830..5266
                     /gene="ftsZ"
                     /locus_tag="VEC56_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXV13177.1"
                     /translation="MRFELDEEKKLTVLRVVGVGGAGGNAVNRMVTAGFRGVEFAAVN
                     TDLQVLRESNAYQKIQIGEALTRGLGSGGDPRVGREAAEESVEAIRACVRGADMVFIT
                     GGMGGGTGTGAAPVVAALAREAGALTVGVVTRPFFFEGTPRLRQGEAGIEALKEAVDT
                     LIVIPNDKLLETSGDDTTMLDAFARADEVLCNATRGISALITETGVVNLDFADVRSVM
                     RNGGAALMGTGVANGPEAAEEAARMAIHSPLLDDVSIQGATSILVNITGPRSLGIKQI
                     SRAATVINSEAGAGAHVFLGTVIDESMPEGEIRVTVIATGFHRPSQAARVAEQRQMEL
                     PRAQAEPRAREWQAAPAAPVAAAPVREPVREPAPAAQVAPPVSPLPIAPPPTVMPAPA
                     MVPVEHAARVAEPVARVEAASERVDTRFDDEEPVLDFPEILARGDSVIEFATEPEDDE
                     PVIMDNGSIRSFNTEVFRIPTFVRKQMD"
     gene            5283..>6799
                     /locus_tag="VEC56_03135"
     CDS             5283..>6799
                     /locus_tag="VEC56_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13178.1"
                     /translation="MSFWTRLLRGATGDQYHRGIRLFNEGNYDEAARVLEELVDHRRA
                     NPFAQLGAFYAAEAHSKLGLAHFHRGELDKARHHFATALSENPRYPDLSYFLGAVEHR
                     AGNHAAAIEHLNRAVELNPDYAEASCLLGLALYDAGYFEQAAEAFGRALELVRSHAHP
                     LSSVLIQQLDARAFDLPALEELRGAVADNSSYTESVREATNAFNVGDYERAVALFAAA
                     VDAHPGYADLHARLGLARMEADDPAGAIECQRRALAINPEYADARYYLGVALFRAGHY
                     GESVAELERVSSAHPAYADVHCQTALARMALGDFALARDALEKSLAISPGYAKAHYLN
                     GLARYALGDTAGAGESLDRALGLKPDLFAVRMERGLYALHDERWSDAAAEFEAVLSSA
                     PRHADARCLLGTALAAMGRDDAAQRCFEEALAINPGYVDARKKLAHLHYANGDTVAAE
                     RHVRAALEAHPDYADLHKILGDVRMQAGDPDGARAAYAHASSINPDYADAVFGLV"
BASE COUNT         1049 a         2230 c         2501 g         1019 t
ORIGIN      
        1 gagcacctcg acttctaccc cgaccttgcg gccatcgagg aggcgttcgt cgagtacgcg
       61 aaccgggtgc cgttctacgg cgcggtgatc ctgtgcgccg acgacgcgcg caccatggag
      121 atcgcgccgc gcatccgccg acgcacggtg acgtactcgc tgggcggtcg ggccgacatc
      181 cagggaagcg tggtcgagcg cggcgcgggc tggacgcgct tcgcggtgtc cgacgcgcgc
      241 ggtccgctgg gggacgtgac gctgcacatt cccggcgacc actatgcgcg caacgcactg
      301 gccgcggtgg cggcggggcg gtggctcgac atccccttcg agcagattcg cgcgggactg
      361 gagagctggc agggcgtggg gcggcgtttc gaggtcaagg gcgaagcggg cggggtgctc
      421 gtcatcgacg actacggcca ccacccgacc gaaatcgcgg ccaccatcca ggccgcgctc
      481 tcgagctacc ggcgccgtct cttcgtgctg ttccagccgc accggtactc gcgcacgcag
      541 gcgctcgcgg ccgagttcgg cacgtgcttc gacggcgcgc atcgcgtatt tctgaccgat
      601 atctacgcgg cgggcgagaa gcccatcgcg ggggtgagca ccgaccgcat cgtcgagcgc
      661 gtcaagcgcg acgggcgcac agaggtcgag cacgtgcgca cctacgaaga cggcgtgcgc
      721 cgcatcgcgc aggacgtgca gcggggcgac atggtcatca cgatgggcgc cggggacgtc
      781 accaaggccg gcgagctcct gctgtcccag ctgggtgctc cggccggaag gaaaggatga
      841 ccggtgacga gcgcaatcga ctggccgggg gcggccgtac gactgtccgc gttcctcgcg
      901 gggcgcgtgc gcgtggacga gccgatgtcg aagcacacga ccatcggcgt gggcgggccg
      961 gcgcgcgtga tggtgttccc gcgcaccccc gcagaagtgg gacgcgtggt gcggctcgca
     1021 ctcgcgctcg ggctccccta cctaccggtg ggcaaggggt cgaacctgat cgtgcgcgac
     1081 gggggctacg acggtgtggt gatccagctc gcggagcaca tgacgcgcgt gcgcgtcgac
     1141 gcgcgcacgg tgtgggcgga gggcggggcg tcgttcgcgg ccctcgcgcg caagatgacc
     1201 aaggcggggc gcacgggcct ggagttcggc atcggcattc ccgggtcggt gggcggggcg
     1261 gtgcgcatga acgcgggtgc cttcggcgga gaagtgaagg acgcgctcgc gcggattcgg
     1321 gtggtggacg aagccggacg cctgcgcgcg atggccgcgt cgcagatcgt gttctcgtac
     1381 cggcacacga gtattcccgc gcgcgccgtg gtggtggaag cggtgtttcg ctgcgcgccc
     1441 ggtaccattc gcaaggacgt gtacgaacgc tcgatcgggc gcaaagaaac gcagccgatc
     1501 tgggagcgca gcttcggcag caccttcgtc aatccgcccg gccgctacgc ggcggagatg
     1561 atcgaggggt gcgggctcaa gggaacgcgg ctcggggggg cgatgttctc ggatttgcac
     1621 gcgaatttca tcgttaacgt ggagggccgc gccagcgcgg ccgacgtcga ggcgctgatc
     1681 gcaacggcgc agcgcgaggt caagaagaag tacggcgtgg atctcaaaac ggaggtgatc
     1741 gtcattggga atcgttagca cggaagccga acggaagcgt accctgcgcg cgcgcgcgcg
     1801 ccgcgtgatc gcggtcttca tcgtgggcgt cctgatcgtg gcgtcgttgt atctctacga
     1861 ttacgtgacc acggcggacc gcttcgccgt cgagcacgtc gagctgtcgg ggctctcgcg
     1921 catcgaccgc gccgaagtgg agcggctggt ggccgacctc aagggccaca acatcctctt
     1981 ggcaccgctc gacgtctacg aggcgcgcct ggaggcgcac ccgcgcatcg cgagtgtcgc
     2041 cgtacggcgc gtgctgcccg accgcgtggt gtacgtggtg accgagcgcg agccggtggc
     2101 gctggtcttc accgaccgct actacgagat cgacgccgac ggcgtggtca tgcccggcga
     2161 cgcgtatacc ggcatgctcg acctgccgac catcaccggg gtctcggcgc gcgacgtccg
     2221 cgcggggcgg gtgtgcgacc agccgctggt gcggggcgcg ctggaagcgc tgagcgtgtg
     2281 ccggtcgatc gggcgcggct tcgccgagga tatctccgag gtgcgcgcga gcgagatcgg
     2341 tctttccatc cgatcgctca aggacgactg cgtcctcctc gtgggcgacg gcgacttcga
     2401 gaagcgtctg cgcaagttct tcttgttgcg cgacgagctc gcgcgacgcg acagcgcgtc
     2461 gggcttgatc gatttgcgct tcgacaatca ggtggtgttg cggagcgata tctaggggcc
     2521 ggttcccggc gccgtgggtg cagtcaaggg aaggcgagca aggaggccgg aaggcatgat
     2581 catgaagagc gcgaggaagc tgtacggtgc gatcgacttg ggcacgacca aggtcgcggc
     2641 cctggtggcg gaggaagccg ggggcgcgct gcgcgtggtg ggagcggggc tggcgccctc
     2701 ggaagggatg cgccaggggg tggtggtgga catcgagcgc gccagcgtgt gcgtggccag
     2761 ggcggtggcc gaagccgagc gcatggcggg tgcgacaccg cgcacgtaca acgtcggtgc
     2821 tgccggcgag cacgtgcgca gcatgaacag ccgcggggtc gtctcggtgg gcgacatcga
     2881 gaaagagatc gcgcccgaag acgtggcgcg cgcgttcgag gcggcgagaa agttttcctt
     2941 gccacaagat cgcgagatca tccataccct accgcaggag tttatcgttg acagccagcg
     3001 gggcattcga cagcctgccg gcatgttcgg cgcgcgtctc gaagcgcgcg tgcacgtggt
     3061 gacggcgtcg cgccccgcgc tcgacaactt ggccaagacg ctcttgctag cgggcgtgga
     3121 agtgggcgac atcgtgctgg agccgcttgc gtcggcgacc gcggtgttgt cggccgacga
     3181 gcgcgagcac ggcgcgatcg tgctcgacgt gggcggaggc accaccgacg tgctggtggt
     3241 ggccgacggg ggcgtactcg cgagcggagt catcggcctc gggggccaca acattacgag
     3301 cgacgtcgcc tacggcctgc ggacgtctcc gcgcgaagcg gagcggctga aggtcgagca
     3361 cggatcggct ctgtcgacgc tggtcgcggg cgacgaagtc gtccaggtca gcatggttgc
     3421 cgggcgcggt ccgcgccggg tggccaagac ggtgctctcg ggaatcatcg agccgcgcgt
     3481 gagcgagctg ttcggtctga tcgaccaaca gatcgcgcag agcggcctca agcgctcgct
     3541 acgcgcgggc gtggtattga ccggcggttc ggcgatgctc accggaatcc gggagctcgc
     3601 cgagcaggtg ttcgacctgc cggcgcgcgt cgcgtcgccc accgggctgg agggattgtc
     3661 ggaagtggtc gcccatccgc ggttctcgac cgcggtcggg cttcttcgcg cgctcgcgcc
     3721 ggccgtaatg ccgggcgagc gagcctcgga gcgcggtggg cgcctcgcgt ccgggctgta
     3781 ttcgttcaag cgtgccatcg ccagttttat ctgacgagaa ggagtcacca tgcgcttcga
     3841 gttggacgaa gaaaagaaac tcacggtcct ccgcgtcgtc ggagtcggtg gcgcgggcgg
     3901 caacgcggtc aatcggatgg tgaccgcggg cttccgcggc gtcgaattcg cggccgtcaa
     3961 caccgacctg caggtcctgc gcgaatcgaa cgcgtatcag aagatccaga tcggcgaggc
     4021 gctgacccgc ggtctggggt cgggcggcga ccctcgcgtg ggacgcgaag ccgccgagga
     4081 aagcgtcgaa gcgatccgcg cttgcgtgcg cggcgccgac atggtcttca tcaccggcgg
     4141 catggggggc ggcaccggaa cgggcgcggc tcccgtggtt gctgcgctcg cgcgcgaggc
     4201 gggcgcgctc acggtcggtg tcgtcacgcg gcccttcttc ttcgagggga ccccgcgcct
     4261 gcgccagggc gaagccggca tcgaggcgct caaggaagcg gtcgacacgt tgatcgtcat
     4321 tcccaacgac aagctgctcg agacctcggg cgacgacacc acgatgctgg acgcgttcgc
     4381 gcgtgcggat gaggtgttgt gcaacgcgac gcgcggcatc tcggccctga tcaccgagac
     4441 gggtgtggtc aacctggact tcgccgacgt gcgcagcgtg atgcgcaacg gcggtgccgc
     4501 cctgatgggc accggtgtgg ccaacggccc cgaggcggcc gaagaggcgg cccgcatggc
     4561 catccacagc ccgctcctcg acgacgtgtc gatccagggc gccacctcga tcctggtcaa
     4621 catcaccggt ccgcgctcgc tcggaatcaa gcagatcagc cgcgccgcga ccgtgatcaa
     4681 ttcggaagcg ggcgcgggcg cccacgtgtt cttgggtacc gtgatcgacg aatcgatgcc
     4741 cgagggtgag attcgcgtga ccgtgatcgc gaccggcttc caccgaccgt cgcaggctgc
     4801 gcgggtcgcc gagcagcgcc aaatggagct gccgcgcgcg caggccgagc cccgtgcgcg
     4861 cgagtggcag gcggctccgg cagcaccggt ggctgccgct ccggtgcgcg agccggtgcg
     4921 tgaaccggcc cccgcggcgc aggtcgcgcc gccggtgtcg ccgcttccga tcgcaccgcc
     4981 gccgaccgtc atgcccgcgc ccgcgatggt tccggtcgag cacgcggccc gcgtggccga
     5041 gccggtggcg cgcgtggaag cggcgagcga gcgcgtggac acgcgcttcg acgacgagga
     5101 acccgtgctc gactttccgg agattctcgc tcgcggcgac tcggtgatcg agttcgcgac
     5161 cgagcccgag gacgacgagc cggtgatcat ggacaacggg tcgatccgct ccttcaacac
     5221 cgaggtgttt cgcatcccga ccttcgtcag aaagcagatg gactagcgaa acggaggaga
     5281 gagtgtcgtt ctggacacgg ctcttgcgcg gtgccacggg cgaccaatac caccgcggca
     5341 ttcgtctgtt caacgaaggc aactacgacg aagccgctcg cgtgctggag gagttggtgg
     5401 accaccggcg cgcgaacccg ttcgcccaat tgggcgcgtt ctacgcggcc gaagcgcatt
     5461 cgaaactggg actcgcgcac ttccaccgcg gcgagctcga caaggcgcgc caccacttcg
     5521 cgaccgcgct ctccgagaat ccgcgctacc ccgacctctc ttacttcctg ggtgcggtcg
     5581 agcaccgcgc cggcaaccac gcggccgcca tcgaacattt gaaccgcgcc gtcgaactca
     5641 accccgacta cgcggaggcg tcctgcctgc tcggtctcgc cctgtacgat gcgggctact
     5701 tcgagcaggc cgccgaggcc ttcgggcgcg cgctcgagct ggtgcgctcg cacgcgcacc
     5761 ccttgagcag cgtgctgatc cagcaactgg acgcgcgtgc cttcgacctg cccgcgctcg
     5821 aagagctgcg cggggcggtg gcggacaact cgagctacac cgagtcggtg cgcgaggcga
     5881 ccaacgcgtt caacgtggga gactacgagc gcgcggtggc gctcttcgcc gccgccgtcg
     5941 acgcgcatcc cggctacgcc gatctgcacg cgcgactggg actggcccgc atggaagccg
     6001 acgaccccgc gggtgccatc gagtgccagc gccgggcgct cgcgatcaac cccgagtacg
     6061 cggatgcgcg ctactacttg ggggtcgcat tgtttcgcgc cggacactac ggcgagagcg
     6121 tggccgagtt ggaacgcgtg tcatcggcgc acccggccta cgcggacgtg cactgccaga
     6181 ccgcactcgc gcgcatggcg ctgggcgact tcgcgctcgc gcgcgacgcg ctggagaagt
     6241 cgctcgccat ttcccccggc tacgcgaagg cgcactacct gaacggcctc gcgcgctacg
     6301 cgctcggcga cacggccggg gcgggcgagt cgctggaccg cgcgctgggc ttgaagccgg
     6361 acctgttcgc ggtgcgcatg gagcgaggcc tgtacgccct gcacgacgag cggtggtccg
     6421 acgcggccgc cgagttcgaa gcggtgctct cgtcggcgcc acgccacgcc gacgcgcgtt
     6481 gcctcctggg gacggcgctg gccgcgatgg gccgcgacga cgccgcgcag cgctgcttcg
     6541 aagaggcgct cgcgatcaac ccgggctacg tcgacgcgcg caagaaactc gcgcacctcc
     6601 actatgccaa cggcgacacc gtcgcggccg aacgccacgt gcgcgcggca ctggaggcgc
     6661 accccgacta cgccgatttg cacaagatcc tgggcgacgt gcgcatgcaa gccggcgatc
     6721 ccgacggcgc gcgcgccgcg tacgcgcacg cgtcgtcgat caatcccgac tatgcggacg
     6781 cggtgttcgg gctcgtgat
//
LOCUS       DASPGK010000109         6239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1433539, whole genome shotgun sequence.
ACCESSION   DASPGK010000109 DASPGK010000000
VERSION     DASPGK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433539"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="VEC56_03140"
     CDS             <1..1346
                     /locus_tag="VEC56_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV13179.1"
                     /translation="VEEFSKWMADGAGAPVGVEISVLSSVSGTLLDNPASYSKMLRAL
                     FSAVQIMAEDRLAHAGVSAPFALVTRDVRDLAKVFGLRRVATDGVQVAAWLLTPTPPS
                     TPAPFRDLGRSIAQARALAFPWRIDTLLEKTRALFAGEERPTAGATLHEIDVAAQILA
                     IAWYRHAVGVGDAVEETAAVKGRLRAAVGRVASLEVVEAYVRMLESAGADSERRQVVV
                     VTVNDDMVKEMSPRFARMGHPVVQKNSMADARPYCEATPPVAVLVDFSTLGMEAARLS
                     VVLRLIPNVMLYAIVDSSDPAVTLDLIDAGFDDVLAPPLDWDVVAARVSRAARGMARK
                     QARESVGFRGTFASFSFVDLIQSLSQSRKSVRVDLLRGDGEGAVIYLDSGRLVHGRAG
                     THAGNEAIYRVVTWADDGSFTVEPVSTFPVANISDPTEAVLMEGCRLFDESRAEV"
     gene            1389..2156
                     /locus_tag="VEC56_03145"
     CDS             1389..2156
                     /locus_tag="VEC56_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXV13180.1"
                     /translation="MGLLGAGALVFAAGVLFGLFGAGGSILLVPILIYVLDLPVKSAL
                     GTSLLILMLTGFVATLAHARARNVSWWIGLRWAALGIVAAYVGGRVAEFIPERILLTM
                     FAIIVVVVSLAMMRRRPLAPAVDPRSIPMGKVVAVGAALGFLTGVMGVGGGFLLVPAL
                     VLLCGMDVKVAVGTSLLIIAVNSAGGFLGFAAHEEFPWRLTLTVAAFNAAGSLVGQRV
                     GRRLPGARLRPAFGVFLLLVGAAMVVQNLLHFASRAR"
     gene            2190..3377
                     /locus_tag="VEC56_03150"
     CDS             2190..3377
                     /locus_tag="VEC56_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXV13181.1"
                     /translation="MFDLNAVQSSLRDLGYDAWLLYDFRGSNPLARRVLGLDEKAINS
                     RRFFYLIPANGAPRKLLHRIETFVLDTLPGDKFVYLRWEELEKAIKELMSPFKRVAME
                     YSPKNAIPYVSHVDAGTVELVKATGVEVVSSGDLITVFEATWDDAQWAMHLEADTHTQ
                     AGYDVAWKLIAERVRAGQSLREVEVQKAIMDHFARHKMTTYHPPIVGVGPHSGDPHYE
                     PVPMKDAEIKENDFVLVDLWAKMDRPRAVYSDLTRVGFVGKTVPEKYTKIFDIVAAAR
                     DAAIACVRSAFKDKRVLHGWEVDDAARNVIKKAGYGDYFVHRTGHSIGQETHGNGANM
                     DNLETHDERRVLKRTCFSVEPGIYLPEFGVRSEINVFVDGDGQVHVTGGLQTEVLPIL
                     AKY"
     gene            3389..3934
                     /locus_tag="VEC56_03155"
     CDS             3389..3934
                     /locus_tag="VEC56_03155"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXV13182.1"
                     /translation="MRIFVVCAAAAALSAALASAPARGQVKQLPTGAFSFQQSITLPG
                     PPDEIFGAITGEIGGWWDHSFSEHPKRFHLEPKVGGCFCEVFDDAGNGVRHAIVTYVD
                     RPKLLRFEGPLGLAGNATHMVFTYQFEPVWGDSTKLTLTGQGSGHVEPGWPDVVAKVW
                     HHFLVERFKPYVEAGKHKAKP"
     gene            4000..>6239
                     /locus_tag="VEC56_03160"
     CDS             4000..>6239
                     /locus_tag="VEC56_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HXV13183.1"
                     /translation="MRKAAVTLALLCVFASLAKAQIVPPAPGVDIPDQVLQSVNEKGA
                     GAFQFKRAWIEKARTTRAARERFIEERGFYQRDLIPAAERPSVAITGNFAVPVFAVKY
                     SDTGADPYPVSTLQARLYSGPHAPRTLTEYYDEISYGDLNLTGTVYGWTLLSGTNAFY
                     AGAGTCNGLCGSANIDNLIIETMNANDGAIDFSQYDNDGPDGLPDSGDDDGYVDFVAF
                     VHPEAGAECGVSGNIWSHRYALSGWTGSPYITNDARTGGGFIRVDDYVIQPAFNCGGA
                     TVIDIGVYCHEYGHAFGLPDLYDTNGGSQGVGHWCLMGSGNWNTPTDPGHMSAWSKNE
                     LGWVDVTVVDNPPANYNIPNVENNRDVFRLDVMQERWRRSSECPLAGTYSMKCGLTAA
                     EAAARNWASGAGYGNGWSETVSHDFNYNGVGPVALTYQYSYNLEATYDFVYGEVTVGP
                     TTSAFAVYDGTSAGLANIDLTPYLGGGPTPYRVSFRMTSDTAFSDEDGSFTTPCAAFL
                     LDNVAVTGGGEAHSANFETRENGWACDMTVPNEHFLVENRKPLGSDVNVWGGGGLAIW
                     HIDDSVAHGSGNTGGPANNRPRGVVLEQADGLLNLENNINRGDAGDPFPGSTNKLAFN
                     GGTTPNSNGYTAASTVTVQLLTGNADPMVATMTGGWPAPAVATALPGTGTSGTTVQVV
                     IGGGGFASGATAELVGASTIPATTVEWVGKDLIAAEFDLTGAANGLYDVVVFNPYGAS
                     DALVDG"
BASE COUNT         1019 a         2053 c         2095 g         1072 t
ORIGIN      
        1 tggtcgaaga gttctcgaag tggatggcgg atggcgcggg tgcgccggtc ggcgtcgaga
       61 tcagcgtctt gtcgagtgtc agcggcaccc tgctcgacaa cccggcgtcg tactcgaaga
      121 tgctgcgcgc gctgttctcc gcggtacaga tcatggcgga ggatcgcttg gcgcacgcgg
      181 gcgtttcggc gcccttcgcg ctcgtgacgc gcgacgtgcg cgacctggcc aaggtgttcg
      241 gtctgcgccg ggtggccacc gacggcgtgc aagtggcggc gtggctcctg acgccgacgc
      301 ccccgtcgac gccggcgccc ttccgcgacc tggggcgctc gatcgctcag gcgcgcgcgc
      361 tggcgtttcc gtggcgcatc gacacgctgc ttgaaaagac ccgggcgctc tttgcgggcg
      421 aggagcgccc caccgcgggt gccaccctgc acgagatcga cgtcgccgcg cagatcctcg
      481 ccatcgcgtg gtatcgccac gcggtggggg tgggcgacgc ggtggaagag acggcggcgg
      541 tgaagggacg cttgcgcgcc gcggtcggac gcgtcgcgtc gctggaagtg gtcgaagcct
      601 acgtgcgcat gctggagtcg gccggggccg acagcgagcg ccgtcaggtc gtggtcgtca
      661 ccgtgaacga cgatatggtc aaggagatgt cgccgcgttt cgcgcgcatg ggacacccgg
      721 tggtgcagaa gaacagcatg gccgacgcgc gcccctattg cgaagcgacc ccgccggtcg
      781 cggtgctggt cgacttctcc accctgggga tggaagcggc ccgattgagc gtcgtgctgc
      841 gcctgattcc gaacgtgatg ctctacgcca tcgtcgactc gagcgacccg gccgtgaccc
      901 tcgatttgat cgacgccggc ttcgacgacg tgctggcgcc cccgctggac tgggacgtgg
      961 tggcggcgcg cgtgagccgt gccgcgcgcg ggatggcgcg caagcaggcg cgcgagtcgg
     1021 tcgggttccg cggcaccttc gcgagcttct cgttcgtcga tctcattcag agcttgtcgc
     1081 agagccgcaa gtcggtccgc gtcgatctgt tgcgcggcga cggcgagggc gcggtcattt
     1141 atctcgacag cggccgcctg gtccacgggc gcgccggtac gcacgcgggt aacgaagcga
     1201 tctatcgcgt ggtcacctgg gccgacgacg gctccttcac ggtcgagccg gtctcgacct
     1261 tcccggtcgc caatatctcc gatcccaccg aagcggtcct catggagggc tgccgcctct
     1321 tcgacgaatc ccgcgccgag gtatagtcgg ccttacgcat ccgagcgcgc ggcgctatac
     1381 tgtcgcgcat gggactactc ggcgccggcg ctctcgtgtt cgcggccggc gtgctgttcg
     1441 ggctcttcgg cgccggcggg tcgatcctcc tcgttcccat cctcatttac gtcctcgatc
     1501 ttcccgtcaa gtccgcgctg gggacctcac tgctcatctt gatgctgacc gggttcgtcg
     1561 ccaccttggc gcacgcgcgc gcgcgcaacg tctcgtggtg gatcggactg cggtgggcgg
     1621 cgctcgggat cgtggccgcg tacgtcggcg gtcgcgtggc tgagttcatc cccgagcgaa
     1681 tcttgctgac gatgttcgcg atcatcgtcg tggtggtgtc gcttgcgatg atgcgccgcc
     1741 gtccgctcgc gcccgcggtc gatccgcgct cgattcccat gggaaaggtg gtcgccgtgg
     1801 gcgcggcgtt gggattcctg accggcgtca tgggcgtggg aggcggattc ctgctggtgc
     1861 ccgcgctcgt gctcttgtgc gggatggacg tcaaggtggc ggtgggaacc tcgctcctca
     1921 tcatcgcggt caactcggcg ggcggcttcc tcggattcgc cgcgcacgag gagtttccgt
     1981 ggcgactcac gctcaccgtg gccgcgttca acgcggccgg aagcctggtc ggtcagcggg
     2041 tcggacggcg gcttccgggc gcgcgcttgc gccccgcgtt cggtgttttc ctgctgctgg
     2101 tgggcgccgc catggtcgtg cagaatctgc tccacttcgc gtcccgcgcg cgctagcatg
     2161 tacacactca caaccgtcgg gagatcacca tgttcgattt gaacgcagtc caatcgtcgc
     2221 tgcgcgacct cggttacgac gcgtggctcc tctacgactt tcgcggcagc aacccgctcg
     2281 cgcgccgggt gctgggcctg gacgagaaag ccatcaactc gcggcgcttc ttctacctga
     2341 ttcctgccaa cggagcgccg cgcaagctgc tccatcgcat cgagaccttc gtgctcgata
     2401 cgctgcccgg cgacaagttc gtctacctgc gatgggagga actggagaag gccatcaagg
     2461 agctgatgtc gccgttcaag cgcgtggcca tggaatactc gcccaagaac gcgattccgt
     2521 acgtgtcgca cgtcgacgcg ggcaccgtcg agctggtcaa ggccacgggc gtggaagtcg
     2581 tctcctcggg cgatttgatc acggtgttcg aagcgacctg ggacgacgcg cagtgggcga
     2641 tgcatctcga agccgacacg cacacccagg ccggctacga cgtcgcctgg aagctcatcg
     2701 ccgagcgcgt gcgcgcgggc cagtccctgc gcgaagtcga ggttcaaaag gcgatcatgg
     2761 accacttcgc gcgccataag atgacgacct accacccgcc catcgtgggc gtgggtccgc
     2821 acagcggcga cccgcactac gagccggtgc cgatgaagga cgccgagatc aaggagaacg
     2881 acttcgtgct ggtcgatctg tgggccaaga tggaccggcc gcgcgcggta tacagcgact
     2941 tgacccgcgt cggtttcgtg ggcaagacgg tgcccgagaa gtacacgaag atcttcgaca
     3001 tcgtcgcggc cgcgcgtgac gccgcgatag cttgcgtgcg gtcggctttc aaggacaagc
     3061 gcgtgctgca cggctgggaa gtggacgacg ccgcgcgcaa cgtcatcaag aaagcgggct
     3121 acggcgatta cttcgtccat cgcaccggcc actcgatcgg tcaggagacg cacggcaacg
     3181 gcgccaacat ggacaatctg gagacgcatg acgaacgccg cgtgctcaaa cgcacgtgct
     3241 tctcggtcga gccggggatt tatctccccg agttcggcgt gcgctccgag atcaacgtgt
     3301 tcgtcgacgg cgacggccag gtgcacgtga cgggcgggct gcagaccgag gtccttccca
     3361 ttctcgcgaa gtactaggag gcgtcgaggt gagaatcttc gtcgtgtgcg ccgccgccgc
     3421 ggcgttgtcc gcagcgctcg cctcagcgcc ggcgcgcgga caggtcaagc agctccccac
     3481 gggcgccttc tcgttccaac aatcgatcac gcttccggga ccccccgacg agatcttcgg
     3541 cgccatcacg ggcgagatcg gcggctggtg ggaccactcg ttctccgagc accccaagcg
     3601 gttccacctc gagcccaagg tgggcgggtg cttctgcgag gttttcgacg acgccggcaa
     3661 cggcgtgcgc cacgcgatcg tcacctatgt cgaccgtccc aagctgctgc gcttcgaggg
     3721 tccgctcggc ctggcgggca acgcgaccca tatggtcttc acctaccaat tcgagccggt
     3781 gtggggagac tcgaccaaac tcacgctcac cgggcaaggc tcgggtcacg tcgaaccggg
     3841 ctggcccgac gtggtcgcca aggtgtggca ccacttcctg gtcgagcgct tcaagcccta
     3901 cgtcgaagcg gggaaacaca aggccaagcc gtagcgtgta tgctataatc atcgaacact
     3961 cgccctagtc gacgtgcccc tcaacaacct ggagagaaca tgcgtaaagc agccgttacg
     4021 ttggccctcc tttgtgtttt tgcatccctg gcgaaggccc agatcgttcc ccccgcgccg
     4081 ggtgtcgata tccccgacca agtcctgcag agcgtcaacg agaagggcgc gggtgctttc
     4141 cagttcaagc gggcttggat cgagaaggcc cgcacgacgc gtgcggcgcg cgagcgcttc
     4201 atcgaagaga gagggttcta ccagcgcgac ttgatccccg cggccgagcg cccgagcgtc
     4261 gccatcacgg gcaacttcgc ggtcccggtg ttcgcggtga aatactccga caccggcgcc
     4321 gatccatatc cggtttcgac gctccaagcc cgcctgtaca gcgggcccca cgcaccccgc
     4381 acgctgaccg agtactacga cgagatctcg tacggcgact tgaatctgac cggcaccgtg
     4441 tacgggtgga cgctcttgtc cggtaccaac gcgttctatg cgggcgcggg cacgtgcaac
     4501 ggcctgtgcg gcagtgccaa catcgacaac ctcatcatcg aaaccatgaa cgccaacgac
     4561 ggcgcgatcg acttcagcca gtacgacaac gacggccccg acgggttgcc cgactccggc
     4621 gacgacgacg gctacgtgga cttcgtcgcc ttcgtgcacc cggaagctgg ggcagagtgc
     4681 ggcgtcagcg gcaacatctg gtcgcatcgc tacgcccttt cgggttggac cggttcgccc
     4741 tacatcacca acgacgcgcg caccggggga ggcttcattc gcgtcgacga ctacgtcatt
     4801 caacccgcgt tcaactgcgg cggcgccacc gtcatcgata tcggcgtgta ctgccacgaa
     4861 tacggccacg ccttcggcct ccccgacttg tacgacacca acggcggctc gcagggtgtg
     4921 gggcactggt gcttgatggg cagcggcaac tggaacacac cgaccgatcc ggggcacatg
     4981 agcgcgtggt cgaagaacga gctcggctgg gtcgacgtca ccgtggtcga caatccgccc
     5041 gcgaactaca acatccccaa cgtcgagaac aaccgcgacg tgtttcgcct cgacgtcatg
     5101 caagagcgct ggcggcgttc gagcgagtgt ccgctcgccg gcacctattc gatgaagtgc
     5161 ggcttgaccg cggcggaagc ggcggcgcgc aactgggcgt cgggcgcggg ctacggcaac
     5221 gggtggagcg agacggtgtc gcacgatttc aactacaacg gcgtggggcc ggtggcactc
     5281 acgtaccaat actcgtacaa tctcgaggcc acctacgact tcgtctacgg cgaggtcacc
     5341 gtcggtccca cgacgagcgc cttcgcggtg tacgatggta cctcggcggg tctagccaac
     5401 atcgacctga cgccgtacct cggcggcggg ccgacgccgt accgcgtgtc gttccgcatg
     5461 acgtccgaca cggcgttttc cgacgaggac ggcagcttca cgacaccgtg cgccgccttc
     5521 ttgctggaca acgttgccgt caccggcggc ggcgaagcgc actccgcgaa cttcgagacg
     5581 cgcgagaatg gttgggcgtg cgacatgacg gtgccgaacg agcacttctt ggtcgagaat
     5641 cgcaagcccc tgggctccga tgtcaacgtg tggggcggag gcggtctcgc gatatggcac
     5701 atcgacgaca gcgtcgcgca cgggagcggc aacacgggcg gaccggcgaa caaccggccg
     5761 cgcggggtgg tgctcgagca ggcggacggg ctgttgaact tggagaacaa catcaaccgg
     5821 ggcgatgcgg gcgatccgtt ccccggcagc accaacaagc tcgcgttcaa cggcggcacg
     5881 acgccgaaca gcaacggcta cacggcggct tcgacggtga cggtgcagct cttgaccggc
     5941 aacgccgatc cgatggttgc gacgatgacc ggcggttggc cggcaccggc ggtcgcgacc
     6001 gcactgcccg gcaccggcac cagcggcact accgtgcagg tcgtgatcgg cggcggcggc
     6061 ttcgcgagcg gcgcgaccgc ggagttggtg ggcgcgagca ccattcccgc gaccacggtc
     6121 gagtgggtgg gcaaggactt gatcgcggcc gagttcgatc tcaccggcgc cgccaatggt
     6181 ctctacgacg tggtggtctt caacccgtac ggtgcctcgg acgcactcgt ggacggctt
//
LOCUS       DASPGK010000110         5749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_379786, whole genome shotgun sequence.
ACCESSION   DASPGK010000110 DASPGK010000000
VERSION     DASPGK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379786"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1195)
                     /gene="sucC"
                     /locus_tag="VEC56_03165"
     CDS             complement(62..1195)
                     /gene="sucC"
                     /locus_tag="VEC56_03165"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXV13184.1"
                     /translation="MKVHEYQAKELFARYGIPVPPGTVVTSAADAEMVAAKVGKPVVV
                     KAQVLVGGRGKAGGVKIAKTPAEAREKASQILGMDIKGETVKKVLVTEAVDIAREIYV
                     AVILDRRAQKPLIMASAEGGVEIEVTAKENPAAIQRAWVDPLYGVHPFAARRLAMALA
                     GDAELAKPMADIIQKLYTLYDDVDASLAEINPLVVTPDRRVLALDAKLNFDDNGLERH
                     PDFEAMRDLEAEDEGEVRARNEGLSFVRLSGNVGCVVNGAGLAMATMDLVKHYGGQPA
                     NFLDIGGSSRPEKVVAALSIILQDKSVKSILFNIFGGITRCDDVARGLVTAFAQMNLK
                     LPVVVRLTGTNEMEAREILRQVPAIHPAETMDEAVKKAIALAA"
     gene            complement(1221..1697)
                     /locus_tag="VEC56_03170"
     CDS             complement(1221..1697)
                     /locus_tag="VEC56_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXV13185.1"
                     /translation="MREVIPEERLREAGDAWRRAGARIVFTNGVFDLLHTGHLQLLED
                     SAAQGDVLVVAINSDASVKRLKGPERPIYPAHERAEILLALRWVDAVTIFDEDTPLET
                     IRRVRPDVLVKGAEYGAGAIVGEDDVVGWGGRVWRYPMREGYSTSRIVDRIRGGEK"
     gene            complement(1697..2701)
                     /gene="rfaE1"
                     /locus_tag="VEC56_03175"
     CDS             complement(1697..2701)
                     /gene="rfaE1"
                     /locus_tag="VEC56_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HXV13186.1"
                     /translation="MTQPTHARPTLSRDTARRYVGRFADSHVVVLGDVMLDRYFWGDV
                     ERISPEAPVPIVHVTRRSRRLGGAANVAANLRALGVRSEVVSVRGSDREGREVARMLT
                     RRGIGSEGLVEAPGRTTTEKVRIIARSQQVIRADFETDDAVDGSARSAIYDRVRSLAA
                     RADALVISDYGKGVVLESEIAEVIAAWRAEEKPVLVDPHVPHFAWYRGATVITPNTRE
                     AQLATGIDYRKGKNPSSVAFDVVERMQLSALLVTRGEDGMSLYYGDRQEVHIPTVAKH
                     VFDVTGAGDTVISVLAAGLATRADVVDAVVMANTAAGEVIKEVGTSTLTADELIAAFE
                     "
     gene            2898..3215
                     /locus_tag="VEC56_03180"
     CDS             2898..3215
                     /locus_tag="VEC56_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXV13187.1"
                     /translation="MNPEPQRTVNIEIGDKEWEGVYSNFVIITHSNSEFVLDFARMLP
                     GAQKAKVFSRIIMTPQHAKALLTTLEQNVKRFETEHGEIKLTPADLSRGPIGFQTPAS
                     KKD"
     gene            complement(3212..4150)
                     /locus_tag="VEC56_03185"
     CDS             complement(3212..4150)
                     /locus_tag="VEC56_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV13188.1"
                     /translation="MGAQMEFNRRAFRQSLEYHAARGALWLAGRLPIEAGRRIGAVIG
                     RAAFDLVRVRRSVSISNIVDSLGVSRAEATRIARAAYTDYGRCLMEFCAFAHLTTAEI
                     LALAECEGWENAERVRAEGKGGLIITAHLANYELVGAWIAARGIPLHYLVGQQTNLRV
                     DDVINEIRHRGSIGIIRRSVALRKVLQALSAGEFVATMPDQDARKGGVMVDFLGRPAS
                     TVRGPALFAIRRGCPILPCFITRQGSRHLLSIEAPLYPAPAASEDEAVRDLTQRYTDR
                     ISARVRLHPDEYFWPHRRWKTKAVQETQSQSVAEIS"
     gene            complement(4160..5251)
                     /gene="dprA"
                     /locus_tag="VEC56_03190"
     CDS             complement(4160..5251)
                     /gene="dprA"
                     /locus_tag="VEC56_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXV13189.1"
                     /translation="MEVNHIALALSMTRNVVPGDWLRLEGTPSEIWDLLQGNAGLAAL
                     GAALGHDVGPVNWGAFDDQLARAEKCGARVVSRWDADYPWRLRHVAAPPPILFVRGCV
                     DCLQRESLAIVGTRAPSAAGMEFAARLARAVAAQDVVVVSGLARGIDTAAHRGALEAA
                     RGGTVAVLGTGVDVVYPAENAALFARVVERGAVVSEQWCGMIGAPHVFPRRNRIISGL
                     SEGVVVVEGGVKSGALITARWALEQGREVGAVPGFPGDFRSAGPNYLIKQGAFVVEDA
                     VDVFANVAGLGVGWVNAGAPRASARETDTLDTEARRVYDLVSHDVDADHVARASGLEV
                     GRVQEILARLEIDGYLVRGESGRFARTGR"
     gene            complement(5276..>5749)
                     /locus_tag="VEC56_03195"
     CDS             complement(5276..>5749)
                     /locus_tag="VEC56_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXV13190.1"
                     /translation="TLISVVSAAHPKIADYPFTTTTPVLGIVRVDEARSFVMVDIPGL
                     IEGAHQGRGMGKEFLRHVERCRILIYMVDVTHPDPATSYRMLRSELMHHDPVLLERKS
                     ILVLTQCDKLPGGVTGVDPKLLELHERTVPISAVSKQGLDDLMRVVSKLLYVSTA"
BASE COUNT          933 a         2078 c         1803 g          935 t
ORIGIN      
        1 gatactgtcc gtctcgcgct gcagtttgag cggagaggga cgatcgcgtc ggcctttccc
       61 cctaagcagc cagcgcgatc gccttcttga ccgcttcgtc catcgtctcg gccggatgga
      121 tggccgggac ctggcgcagg atctcgcgcg cttccatctc gttggtgccc gtcagtcgca
      181 ccaccaccgg tagcttcaag ttcatctgtg cgaacgcggt caccaagccg cgtgcgacgt
      241 cgtcgcaccg cgtaataccg ccgaaaatat tgaacaggat cgacttgaca cttttgtcct
      301 gcaagatgat ggagagcgcc gcgaccacct tctccggccg cgaactgccg ccgatatcga
      361 ggaagttcgc gggctggccg ccgtagtgct tgaccaaatc catcgtcgcc atggccaggc
      421 cggcgccgtt cacgacgcag cccacgttcc ccgacaggcg cacgaaggaa agcccctcgt
      481 tgcgggcgcg cacttccccc tcgtcttcgg cctccaagtc gcgcatggct tcgaagtcgg
      541 gatgccgctc gagcccgttg tcgtcgaagt tgagcttggc gtcgagggcc agcacgcggc
      601 ggtccggcgt caccaccagc ggattgatct ccgccaggct tgcgtcgacg tcgtcgtaga
      661 gcgtgtagag cttctgaata atgtcggcca tcggcttggc cagctcggcg tcgcccgcga
      721 gcgccatggc cagccggcgc gcggcgaacg gatgcacgcc gtacaacggg tcgacccacg
      781 cgcgctggat cgcggcggga ttctccttcg cggtcacttc gatctccacg ccgccttccg
      841 ccgaagccat gatgagcggt ttctgcgcgc gtcggtccaa gatcacggcc acgtagatct
      901 cgcgcgcgat gtcgacggcc tcggtgacca gcaccttctt gaccgtctcg cctttgatgt
      961 ccatgccgag aatctgcgac gccttctcgc gcgcctcggc cggcgtcttc gcgatcttga
     1021 cgccacccgc tttgccgcgg ccgcccacca gtacttgcgc tttgaccacc accggcttgc
     1081 ccaccttggc cgccaccatc tccgcgtccg ctgccgacgt caccaccgtc cccggcggca
     1141 ccgggattcc gtagcgcgcg aacaactcct tggcctggta ctcgtgtact ttcatgatgc
     1201 tcctcgagag tgcgacggcg tcacttttcg ccgccgcgaa ttcgatcgac gatgcgcgag
     1261 gtcgaatagc cttcgcgcat cggatagcgc cacacccgcc caccccagcc cacgacgtcg
     1321 tcctcgccca cgatcgcgcc cgcgccgtac tcggcaccct tgacgagtac gtcggggcgc
     1381 acgcggcgga tggtctcgag cggcgtatcc tcgtcgaaga tcgtcaccgc gtccacccac
     1441 cgcagcgcca agaggatctc cgcgcgctcg tgcgcgggat agatgggccg ctcgggcccc
     1501 ttgagccgct tcaccgacgc gtcgctgttg atcgccacga ccagcacgtc gccctgtgcg
     1561 gcggaatcct ccagcaattg gaggtgcccg gtgtgcaaca gatcgaagac gccgttggtg
     1621 aacacgatgc gcgccccggc gcgccgccag gcatcgcccg cctcgcgcag ccgctcctcc
     1681 ggaatgacct cgcgcatcac tcgaaggccg cgatcagctc gtcggccgtg agcgtcgacg
     1741 tccccacttc cttgatcact tcgccggccg ccgtgttggc catgaccacc gcgtcgacca
     1801 cgtcggcgcg cgtggccagt ccggccgcga ggacgctgat caccgtgtcg ccggcaccgg
     1861 tgacgtcgaa cacgtgcttg gcgacagtcg ggatgtggac ttcctggcgg tctccgtaat
     1921 agagactcat cccgtcctcg ccgcgcgtca ccaagagcgc cgagagctgc atgcgctcga
     1981 ccacgtcgaa ggcgaccgac gacgggttct tgcccttgcg gtagtcgatg ccggtcgcga
     2041 gctgcgcttc gcgcgtgttg ggtgtgatca cggttgcacc ccggtaccac gcaaagtgcg
     2101 gcacgtgcgg atcgaccagc acgggcttct cttcggcgcg ccacgcggcg atgacctcgg
     2161 cgatctcgga ttcgagcacg acgcccttgc cgtagtcgga gatcacgagc gcgtccgcgc
     2221 gcgcggcgag cgaacgcacg cggtcgtaga tggcgctgcg cgcggacccg tccaccgcgt
     2281 cgtcggtctc gaagtcggcg cgaatcacct gctggctgcg cgcgatgatg cgcacctttt
     2341 ccgtcgtcgt gcgcccgggc gcttcgacca gtccttcgct cccgatcccg cggcgcgtca
     2401 gcatgcgcgc gacctcgcgg ccctcgcggt cgctgccgcg caccgagacc acttcggagc
     2461 gcacccccag cgcgcgcaga ttggcggcca cgttggccgc accccccagg cggcggctgc
     2521 gccgcgtcac gtgcacgatg ggcaccggcg cttccggcga gatgcgctcc acgtcgcccc
     2581 agaagtagcg atcgagcatg acgtcgccga gcacgacgac gtgcgagtcc gcgaagcggc
     2641 cgacgtaccg gcgtgccgtg tcacggctca acgtgggtcg tgcgtgggtg ggttgggtca
     2701 aggtcgtcct caaaaaaaag gccccgctcg aggctccccg aggcgggaca ggttcgttgc
     2761 gggcgggccc gtaaacagcg cgcaccctat cacgcgcgag cgggtcctgt caagcgggcc
     2821 gcccggcttg ctaaccccaa cccagcgtgg tagattcggc cgcgaccggt gatcggcgtg
     2881 tgacccaagg aggccccatg aaccccgagc cccagagaac cgtcaatatc gagatcggcg
     2941 acaaggagtg ggaaggcgtc tactccaact tcgtcattat tactcactcc aactcggagt
     3001 tcgtcttgga cttcgcgcgc atgctgccgg gggcgcagaa ggccaaggtc ttctcgcgca
     3061 tcatcatgac gccccagcac gccaaggcgc tcctcacgac gctggagcag aacgtcaagc
     3121 gcttcgagac cgagcacggc gagatcaagc tcaccccggc cgacctctcc cgggggccga
     3181 tcggcttcca gaccccggcc tcgaaaaaag attagctgat ttcggcaacc gactgcgact
     3241 gtgtctcttg aacggccttt gtcttccagc gccggtgcgg ccagaagtac tcgtcggggt
     3301 gcagccggac acgcgcggaa atgcgatccg tgtagcgctg ggtgaggtcg cggacggctt
     3361 cgtcctcgct ggcggccggg gccgggtaga ggggggcttc gatcgagagg agatgacgcg
     3421 acccctgccg cgtgatgaag cacggcagga tcgggcagcc gcgccgaatc gcgaacagcg
     3481 ccggaccgcg cacggtcgac gcggggcgtc ccaaaaagtc gaccatcaca ccgcccttgc
     3541 gcgcgtcttg gtccggcatg gtggcgacaa actctccggc tgataacgcc tgcagcacct
     3601 ttcgcagcgc caccgatcgt cggatgatgc cgatgctccc gcggtggcgg atttcgttga
     3661 tcacgtcgtc gacccgcaga ttggtctgct ggcccaccag gtaatggagc ggtattcccc
     3721 gcgccgcgat ccacgcgccg accaactcat agttcgccag gtgtgcggtg atgatcaggc
     3781 cgcccttgcc ctccgcgcgc acgcgctcgg cgttctccca cccctcgcac tccgccaggg
     3841 cgaggatttc ggccgtcgtg aggtgagcga acgcgcagaa ctccatcaag caccggccat
     3901 agtccgtgta ggcggcgcgc gcgatgcgcg tcgcttccgc gcgcgaaacg ccgagcgagt
     3961 ccacaatatt agaaatgctc acggaacgcc gcacgcgcac caggtcgaag gcggcgcgcc
     4021 cgatcacggc gccgatgcga cggccggctt cgatgggcag acggcccgcg agccacaagg
     4081 cgccccgcgc cgcgtggtac tcgagcgatt ggcgaaatgc gcgccgattg aactccattt
     4141 gggctcccat gactcctgat tagcgacccg tgcgcgcgaa gcgtccgctc tcgccccgca
     4201 cgaggtaccc gtcgatctcc aggcgcgcga gaatctcctg cacgcgaccg acctcgagcc
     4261 ccgacgcgcg ggccacgtgg tcggcgtcga cgtcgtgcga gacgagatcg tagacgcgcc
     4321 gcgcttccgt gtcgagggtg tcggtttcgc gcgcgctcgc gcgcggcgca cccgcattca
     4381 cccaccccac accgagtccc gccacgttcg cgaatacgtc gacggcgtct tccaccacga
     4441 aggcgccctg tttgattagg tagttggggc ccgcgctgcg aaaatccccc gggaacccgg
     4501 gtacggcacc tacttcgcgc ccctgctcga gcgcccagcg cgcggtgatc aaggcaccgc
     4561 tcttgacgcc cccttcgacg accaccacac cttcgctcaa gccgctgatg atccggttgc
     4621 gccgcgggaa cacgtgcggt gcgccgatca tcccgcacca ttgctccgaa acgacggcgc
     4681 ctcgttccac cacgcgcgcg aagagcgctg cgttctccgc cggatagacg acgtcgacgc
     4741 ccgtccccag aaccgccacg gtaccgccgc gcgcggcctc gagcgccccg cggtgggcgg
     4801 cggtatcgat cccgcgcgcg agcccgctca ccaccacgac gtcctgcgcc gccacggcgc
     4861 gcgccaaacg cgccgcgaat tccatcccgg cggcactggg cgcccgcgtg ccgacgatcg
     4921 cgagcgactc cctctgcaga cagtcgacgc acccgcgcac gaacagaatc ggcggcgggg
     4981 cggcgacgtg gcgcagccgc cacggatagt ccgcgtccca gcgcgagacg acgcgcgcgc
     5041 cgcatttttc cgcgcgcgcg agctggtcgt cgaacgcccc ccagttgacg gggcccacgt
     5101 cgtggcccag ggccgcgccc aacgcggcaa gccccgcgtt gccctgcaag agatcccaga
     5161 tctcgcttgg cgttccctcc aggcgcaacc agtcgcccgg cacgacgttg cgcgtcatcg
     5221 acaacgccag cgcgatgtga ttcacctcca ccccacacct ccctcgacct cggcgctacg
     5281 ccgttgagac gtataacaac ttggacacca cccgcatcaa gtcgtccaaa ccctgcttgg
     5341 agacggccga gatcggcacc gtgcgctcgt gcagctcgag gagtttgggg tcgacccccg
     5401 tgacgccacc cgggagcttg tcgcactggg tcaggacgag gatgctcttg cgctcgagca
     5461 gcaccgggtc gtggtgcatg agctcgcttc gcagcatgcg atagctcgtc gccggatcgg
     5521 ggtgcgtcac atcgaccata taaatgagaa ttcggcagcg ctcgacgtgg cgcaggaact
     5581 ccttacccat gccgcgtcct tggtgggccc cctcgatcag ccccggaatg tcgaccatga
     5641 cgaacgaccg cgcctcgtcc acgcgcacga tgcccaacac cggggtcgtg gtcgtgaacg
     5701 gatagtcggc gatcttgggg tgcgcagcgc tgaccacgct aatcagcgt
//
LOCUS       DASPGK010000111         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_146089, whole genome shotgun sequence.
ACCESSION   DASPGK010000111 DASPGK010000000
VERSION     DASPGK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146089"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..1603
                     /locus_tag="VEC56_03200"
     CDS             428..1603
                     /locus_tag="VEC56_03200"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HXV13191.1"
                     /translation="MRRLFQVILVVLAVALALCAALAGPLARLVDACFNRTTNTSRPP
                     TSAAAIELHDRLFVADLHCDAVLWDRGLLTRSTRGHADLPRLQEGRVALQVFSLPNAY
                     PLLSNYRRTPARPDVLGAAAFAHRWPRATWASPVARALHQARVFHDAVARSEGGLVVV
                     TSASGLAEFGTQRLATPPPVGAILAVEGLHLARDDLRSIDYLFMTGVRVFGLAHMGDN
                     AVGGSAHGWRKHGLTDFGRRVVAHIDSLGGIVDLAHASDATIADVLAMPTVPVLVSHT
                     GVDGTCPGPRNLSDDQLARIAARGGVIGIGFWNAAVCGHDPAAIARAVRHAVRVAGID
                     AVALGSDFDGAVRTPFDASGMVYLTEALMAEGFDAHQIARIMGLNVLDFFLRSLPPE"
     gene            complement(1609..>2312)
                     /locus_tag="VEC56_03205"
     CDS             complement(1609..>2312)
                     /locus_tag="VEC56_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV13192.1"
                     /translation="DVGCGMGQLSRAIARAAGKRGRVVGIERSREQIEEGRRQAAAAK
                     EADTVDVREGDAGTPPLSDDEWGTFDIAHARFVLEHVADPARVVRAMVKAVRPGGRIV
                     LEDDDHELLRFHPAVPEFESAWRAYMRAYQAVGNDPVIGRKLPALLVEAGARPSASDW
                     LFFGACQGSPTFPIIVSNCRSIMAGARASVIGYGGLSADAFEAALVAFDRWSREPGAA
                     LWYCTFWAEGVRPNA"
BASE COUNT          340 a          843 c          732 g          397 t
ORIGIN      
        1 ccaacatgcg gatcgaactc gtcgacctac tctggtggct taggaagcga taactcgcgc
       61 ggtgttcctg gatgcccgtt tcgcgagcgt gacacctggg aacaccgccc gccacctctc
      121 ccccgccgga ctcgaccctc cttcgagtag tacacgaaga aaagaccact tcgtcccgtt
      181 ttcaggcttc aaacaaccct attgactagg ttgacaattt tcggaaagcg cgactatact
      241 ccactcttgc aacgagagtc ctccctcttc tgcgcgtgcg cctgctcttc aagctagtgg
      301 atagcgaagc ggttgccgca tgggcctctg acgggcgaaa cagcgatacg gcagttcggg
      361 ggcgaactct cgttcttttt ttgcctacct ttgaggcacg atccgtcggc atcgtgggcc
      421 gattctcatg cgtcgcctct tccaagtcat actcgtggtt ctggcggtag ctctcgcgct
      481 ctgtgcggcg ttggccggtc ccctcgcgcg tctggtcgac gcatgcttca accgaacgac
      541 gaacacgtcc cgccccccca catccgccgc cgcgatcgag ctccacgacc gcctcttcgt
      601 cgccgatttg cactgcgacg cggtgctgtg ggatcgcggc ctcctgaccc ggtcgacccg
      661 cggccacgcc gacctcccgc gtctccaaga ggggcgcgtg gcgctccagg tcttctcgct
      721 tcccaacgcc tacccgctcc tctccaatta ccgccgcacg ccggcaaggc ccgatgttct
      781 cggggcggcc gccttcgccc accgatggcc gcgcgcgacc tgggcctcgc cggtggcccg
      841 cgcgctccat caagcgcgcg tgttccacga cgcggtcgcc cggtccgagg gcggcctggt
      901 ggtggtgacg agcgcgagcg gcttggccga attcggcacc cagcgcctcg cgactccgcc
      961 gcccgtcggc gctattctcg cggtcgaagg cttgcacctt gcgcgcgacg atcttcgatc
     1021 gatcgactat ctgttcatga ccggcgtgcg cgtgttcggc ctcgcccaca tgggcgacaa
     1081 cgccgtcggc ggttcggcgc acgggtggcg aaagcacggc ctcaccgact tcgggcgccg
     1141 tgtggtcgcg cacatcgact cgctgggcgg catcgtcgac ctcgcgcacg cgtcggatgc
     1201 gaccatcgcc gatgtgctcg cgatgccgac ggtccccgtg ctcgtctcgc acaccggagt
     1261 cgacgggacc tgcccggggc cacgcaacct ctcggacgac caactcgcgc ggatcgcggc
     1321 gcgcgggggc gtcatcggga tcggattctg gaacgccgcg gtatgcggcc acgaccccgc
     1381 agccatcgcg cgggcggtgc gccacgcggt gcgcgtggcg ggcatcgacg cggtggcgct
     1441 gggatccgac ttcgacggag cggtgcgcac gccgttcgac gcgtccggaa tggtctatct
     1501 gaccgaggcc ttgatggcgg agggattcga cgcgcaccaa atcgcgcgca tcatggggct
     1561 caatgtgctg gacttcttcc tgcgctcgct gccgcccgag taacgggctc acgcgttcgg
     1621 cctgacgcct tccgcccaga acgtgcagta ccacagcgcc gcacccggct cacgactcca
     1681 gcgatcgaag gcgaccagcg ccgcctcgaa ggcgtccgcc gaaagtccgc cgtacccgat
     1741 caccgacgcg cgcgcacccg ccatgatgct ccggcaattc gagacgatga ttggaaacgt
     1801 gggcgaaccc tggcaggccc cgaaaaacag ccagtcgctc gcggacggtc gtgctcccgc
     1861 ttcgaccagc agcgccggca gcttgcgtcc gatcacgggg tcgttgccga ccgcttggta
     1921 ggcgcgcatg tacgcgcgcc acgccgattc gaattcggga acggcggggt ggaagcgaag
     1981 cagctcgtgg tcgtcgtctt cgagcacgat acgcccgccg gggcgcaccg ccttgaccat
     2041 cgcgcgcacg acgcgcgcgg ggtcggcgac gtgctcgaga acgaagcgcg cgtgcgcgat
     2101 gtcgaacgtg ccccactcgt catcggagag cggcggggtg cccgcgtcgc cctcgcgcac
     2161 gtcgaccgtg tcggcttctt tggcggccgc ggcctgccgc cggccttcct cgatctgctc
     2221 gcggctgcgc tcgatcccca ccacgcgccc gcgctttccc gccgcgcgcg cgatcgcgcg
     2281 cgaaagctgc cccatgccgc agccgacgtc ga
//
LOCUS       DASPGK010000112         5529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1316818, whole genome shotgun sequence.
ACCESSION   DASPGK010000112 DASPGK010000000
VERSION     DASPGK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316818"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..3158)
                     /gene="tssH"
                     /locus_tag="VEC56_03210"
     CDS             complement(366..3158)
                     /gene="tssH"
                     /locus_tag="VEC56_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HXV13193.1"
                     /translation="MAKQSHLSSLRNLLGRCNSTTVEILLDATALCRRLGHAEILPEH
                     VVDSALARSDSDFGLLCRHFGLDLSSLKSDVNRVLNEQKTIGVPRPPMSAGTVELLER
                     AWNRVSTGAGRGFIRSGDLYWAIATDANDKVSRPRRRIREATKVEELEKDFDRLVAHA
                     SEGVGAVEGGAGAAATDAPSAMPTGGGGTSEAPARGDGGYVAKYCVNFTLLAEQGKLD
                     PVFGRDEEIREAIDILARRRKNNPILVGEPGVGKTAIVEGLALRIVQGDVPANFANFH
                     VIGLDLGLMQAGASVKGEFEKRLKGVITEIKESPVPTIFFIDEAHTLVGAGGQAGGSD
                     AANLLKPALARGELRSIAATTWSEYKQYFETDPALTRRFQLVHVKEPDLDDAKTMLRG
                     LRAKYEQAHGIRILDSAIEGAVEAAKRYVAGRYLPDSAIDLIDTACAKVKNVLQSKPA
                     VIDQIERDLTDLEIERTSVLREVEEGRAGAKTRLQAVDGRRSELSARLEAQRERWQVE
                     RVKVDAIHEVARQVAQARERLEEARQALDFEKAATIEREELAKLGEQLGREIDALKEL
                     QGDEPLLHAEVDSDTVLSVVAQWTGVPVHKMAAGDAAKIASLERHLGERIKGQEHAVQ
                     LISEVLKTHKAGIGDPNRPIGVFLCLGPSGVGKTEIALAVADILFGGDSFITKINMSE
                     YSSGFSESRLIGAPPGYKGYGKGGVLTEAVRQKPYSVVLLDEVDRADSQVWSLFYSPF
                     EKGEMNDGEGRKINFRNTCIFLTSNLGSDAILKWAEMRAKQAAPAAAGKVDADETAEE
                     QLTLEALTEYLKDVAGKTFTMPLLARMTLVPFIPLDREALKEIVRLKLAKVKNQLKSQ
                     RKLEFGWDEGVPDWIVDRCETAGFGARLIDQTVQTDMLPGVTRELLAALARGESPTSV
                     HVSVGANGAGLQVRVS"
     gene            complement(3124..4203)
                     /locus_tag="VEC56_03215"
     CDS             complement(3124..4203)
                     /locus_tag="VEC56_03215"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HXV13194.1"
                     /translation="MDLSARVATHARKYTYYKLLHDLERLGCYGPERLKVRPVGSRMF
                     PARDVCDVHLGGTDGAPDELPRAEVVVSFGGLFGVDSPLPMYFDDILDEDTPASANLR
                     GLLTLIGNRYYHLLYEIWSRMAPMFCGEQALENFVRKPALSICGLGHLKGARINVLAG
                     AAFLVQGMRNRWGLASMLESFLGAPVYVADFDPAQVPLRDRERTRLGDADTAVLGRTF
                     RATPYRDTFTTHVRVVCGPLDCDKYIGLLPGGELHAVLSYLTFRYLPADLEYRVELVL
                     AAEAVKELRFQLRPGGLRLGATTVLGASRIRRELRIVREGISLASRPEQLREIIEFIE
                     SRGPARSTGTRNEQDGEAIASLKSA"
     gene            complement(4194..>5529)
                     /locus_tag="VEC56_03220"
     CDS             complement(4194..>5529)
                     /locus_tag="VEC56_03220"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HXV13195.1"
                     /translation="SAVELGETSSVDRTALRITLSSRNGTPLVAPDPMPAELPILLFW
                     SDLAHTCTVRRCLANMPAPPRFVAIAAKGGQESVIEKKVALRVRRPMEHEPMLTAGRR
                     AFPGFRMMREFFALPQRFMQFVITGLDTCEELQEAGALRIEATLPLSIRDLPPFDVKN
                     FLPFTSAVCNEWSGSAKPIHYDGTRETYALEADHAGGAEILRVESVRMLPRGADSFTP
                     VEPFNYYNREIPSYRPGSESYHLSESRRRVRWNLDLNNLAPPGTQVVVGADLALANAD
                     AHEVLGARAVLANYEGVPEDLAANTIAPSTEFTPALEAEGQYWQLVDMVASSLSAMAS
                     PLRLQMFLLHLNRTDNHIMAGAIRGIKGVELRMTTTTLRQQPLKVATMQVDIDRGEYL
                     NSGFIEVFFDAFYGLLEEFVPLNCSLELEVRDADTKKVERRWLPEGARYTWT"
BASE COUNT          911 a         1864 c         1737 g         1017 t
ORIGIN      
        1 ggtgctctcg atggtcactc gcccgccgtt gtcaccattg cgtccagcgt tcacgagcgt
       61 ctcgatcgag tcgacttgct gaatcttgaa ggtaactgcg aagctcccca cgtcgggcgc
      121 gtcactgtcc tcgaacgacg ccgacagcgt agtcgtttgc accccacaga cgttcaccga
      181 gctcggaact gcctgtgtaa cttccgccgt gacgaccgga gccgacaagt tggggggaaa
      241 cgggccccag tcgttgtttt cctcatcgca ccccgcgacg agcgcgagcg tcgcaatcac
      301 cattaaccac gcagatcgtt tgtgctttga caacgtgttt tcctccctgt ctcgagccga
      361 catccctacg acacccgaac ctgcaacccg gccccgttcg cccccaccga cacgtgcacc
      421 gaggtcggcg attcgccccg ggccaacgca gcgaggagct cgcgagtcac ccccgggagc
      481 atgtcggttt gcaccgtctg atcgatgagg cgtgcgccga agcccgccgt ctcgcagcga
      541 tcgacgatcc agtcgggaac accctcgtcc cagccgaact cgagcttacg ctgcgatttg
      601 agctggttct tcactttggc caacttgagt ctcacgatct ctttgagcgc ctcgcggtcg
      661 aggggaatga acgggacgag cgtcatgcgc gcgagcagtg gcatggtgaa cgtcttgccc
      721 gccacgtcct tcaggtattc cgtgagcgct tccagcgtga gttgctcctc cgccgtttcg
      781 tccgcgtcca ccttgccggc ggcggcaggc gccgcctgtt tcgcgcgcat ctcggcccat
      841 ttcagaatgg cgtcgctgcc gaggttcgac gtgagaaaaa tgcacgtgtt gcggaagttg
      901 atcttgcgcc cctcgccgtc gttcatctca cccttctcga agggcgaata gaacaagctc
      961 catacttgcg agtccgcgcg gtccacttcg tcgagcaaca ccaccgaata gggtttctgc
     1021 cggacggctt cggttagcac gccgcccttg ccgtagccct tgtagcccgg cggcgcccca
     1081 atcagccgcg attcgctgaa tcccgagctg tactccgaca tgttgatctt ggtgatgaac
     1141 gaatcgcccc cgaacaatat gtcggcgacc gccagcgcga tttcggtctt tcccacgccc
     1201 gacgggccca ggcacaggaa cacgccgatg ggacggttgg ggtccccgat gccggccttg
     1261 tgcgtcttga gcacttccga tatcagttgc accgcgtgct cttggccctt gatgcgctcg
     1321 cccaggtgac gctcgagcga cgcgatcttg gccgcgtcgc cggccgccat cttgtggacc
     1381 ggcactcccg tccactgcgc cacgaccgac agcaccgtgt ccgaatcgac ttccgcgtgc
     1441 aacagcggct cgtcgccttg tagctccttc agcgcgtcga tctcgcggcc aagctgttct
     1501 cccagcttcg cgagttcctc ccgctcaatt gtagcggctt tctcgaagtc gagcgcctgg
     1561 cgcgcctcct ccagtcgctc gcgcgcttgc gcgacctgac gtgccacctc gtggatcgcg
     1621 tcgaccttga cgcgttccac ctgccagcgt tcgcgctgcg cctccaagcg tgcgctgagc
     1681 tcgctgcggc gaccgtcgac ggcctgcaag cgggtcttcg cgcccgcgcg accctcttcg
     1741 acttcccgaa gcaccgacgt gcgctcgatc tcgaggtcgg tgagatctcg ttcgatctgg
     1801 tcgatgaccg cgggtttgct ctggagcacg tttttcacct tggcgcacgc agtatcaatg
     1861 agatcgatcg cggagtcggg gagatagcgg cccgcgacgt agcgcttggc cgcctccacc
     1921 gcgccctcga tggcactgtc caggatgcgg atcccgtggg cttgttcgta tttcgcgcgc
     1981 agtccgcgca gcatagtctt ggcgtcgtcc aagtcgggct ccttgacgtg aacgagttgg
     2041 aagcgccgcg tgagcgccgg gtcggtctcg aagtactgct tgtactccga ccacgtggtg
     2101 gccgcgatgg agcgaagctc tccgcgcgcg agcgcgggct tgagcagatt ggcggcgtcg
     2161 ctgccgccgg cctgtccgcc tgcgcccacc agcgtatggg cttcgtcgat gaaaaagatg
     2221 gtgggaaccg gggattcctt gatctcggtt atgacgccct tgagccgttt ctcgaactcg
     2281 cccttgacgc tggcgccggc ctgcatcagc ccgaggtcca ggccgatgac gtggaagttg
     2341 gcgaaattgg cgggaacgtc gccctgcacg atgcgcagcg ccaacccctc gacgatggcg
     2401 gtcttgccca ccccgggctc tcccacgaga atgggattgt tctttctccg acgggcgagg
     2461 atgtcgatcg cttctcgaat ttcttcgtcg cggccgaaca cggggtcgag cttgccctgc
     2521 tcggccagga gcgtgaagtt cacgcagtac ttcgcgacgt agccaccgtc gccgcgcgcc
     2581 ggtgcctcgg aggtaccgcc cccgcccgtc ggcatcgccg acggtgcgtc cgtcgccgcc
     2641 gcccccgctc cgccctccac cgcacccacg ccctcgctcg cgtgcgcgac caaacggtcg
     2701 aagtccttct ccagttcttc gaccttggtc gcttcgcgaa tccgacgccg cggacgggag
     2761 accttatcgt tcgcgtcggt cgcgatcgcc cagtacagat cgccgctgcg gatgaagcct
     2821 cgcccggcgc cggtcgacac gcgattccat gcgcgttcca ggagctcgac agtgcccgcg
     2881 ctcatgggtg gcctcggcac gccgatggtc ttctgttcgt tcagcacccg attgacgtcg
     2941 gacttgagcg acgataggtc gagaccgaag tgccggcaca ggagtccgaa gtcggagtcg
     3001 gaccgggcca gcgcgctgtc cacgacgtgc tccgggagga tctcggcgtg ccccagccgg
     3061 cggcacaagg cagtcgcatc cagcaggatc tcgaccgtcg tgctgttgca gcgtccgagt
     3121 agattacgca gacttgagag atgcgattgc ttcgccatct tgttcgttcc tcgtccctgt
     3181 agaacgcgcg gggccccgtg actcgatgaa ctcgatgatc tcccgaagtt gttccggccg
     3241 tgaggccagg ctgattccct cgcggacgat gcggagctcg cgccggatcc gcgaggcgcc
     3301 caaaacggtg gtcgcaccca ggcgaagccc gcccggcctc aactggaagc gcaactcttt
     3361 taccgcctcg gcggccagca ccaactcgac ccggtactcg aggtcggccg gcaggtaacg
     3421 gaacgtcagg tacgacagaa ccgcgtggag ctcgccgccg ggcaggagac cgatgtactt
     3481 atcgcagtcc agcgggccgc acacgacgcg cacgtgcgtc gtgaaggtgt ctcggtacgg
     3541 cgtcgcgcga aaagtccggc cgagcacggc cgtgtcggcg tcgccgaggc gcgttcgttc
     3601 ccggtcgcgc agcggcactt gcgcggggtc gaagtccgcc acgtacaccg gcgcgcccag
     3661 aaagctctcc agcatggagg ccaggcccca gcgattgcgc atcccctgga cgagaaacgc
     3721 cgcgcctgcg agcacgttga tacgtgctcc tttcaagtga ccgagaccgc agatggacaa
     3781 cgccggcttc cgcacgaagt tctccaacgc ctgctcaccg cagaacatcg gtgccatccg
     3841 actccagatc tcgtagagca ggtgatagta gcgattcccg atcagggtca gcaggccgcg
     3901 cagattggcg ctcgcgggcg tatcctcgtc caggatatcg tcgaaataca tcggcagggg
     3961 ggagtcgacg ccgaagagac cgccgaacga gaccacgacc tcggctctcg gaagctcgtc
     4021 cggcgcgccg tccgtgccgc ccaagtgaac gtcgcaaacg tcccgcgccg gaaacatacg
     4081 cgatcccacc gggcgcacct tgagtcgctc gggcccgtag caccccagcc gctccagatc
     4141 gtggagcagt ttgtagtagg tgtactttct cgcgtgggtc gcgacccggg cgctcaagtc
     4201 cacgtgtacc gggctccttc cggcagccaa cgacgctcga ctttcttggt atcggcgtcg
     4261 cgaacttcga gttccaacga gcaattcagc gggacgaact cttcgagcag gccgtagaac
     4321 gcgtcgaaga acacctcgat gaacccactg ttcaagtact cgccgcgatc gatgtcgacc
     4381 tgcatcgtgg ccaccttgag cggctgctgg cgcaaggtgg tcgtcgtcat gcgaagctct
     4441 acgcccttga ttccgcgaat ggcaccggcc atgatgtggt tgtcggtgcg gttcaaatgg
     4501 agcaggaaca tctgcaagcg cagcggactc gccatggcgg agagactgct cgcaaccatg
     4561 tcgaccagct gccagtactg cccttcggcc tcgagtgcgg gcgtgaactc cgtgctgggg
     4621 gcgatcgtgt tggccgccag atcctcggga acgccctcgt agttggccag cacggcgcgg
     4681 gcccccagca cttcgtgcgc gtccgcattg gcgagcgcga ggtcggcgcc gacgaccacc
     4741 tgcgtgcccg gcggcgccag gttgttcaag tcgaggttcc agcgaacccg gcggcgcgac
     4801 tccgacaggt ggtacgactc ggagccgggc cggtagctcg gtatctcgcg gttgtagtaa
     4861 ttgaagggct cgacgggcgt gaacgaatcg gcgccgcgcg gcagcatgcg cacgctctcg
     4921 acccgcagga tctcggcgcc gccggcgtgg tcggcttcta gcgcgtaggt ctcgcgcgtg
     4981 ccgtcgtagt gaatcggctt ggcactccca ctccactcgt tgcacaccgc cgacgtgaac
     5041 ggtaggaagt tcttcacgtc gaaggggggc aggtcgcgaa tcgacagggg gagagtggcc
     5101 tcgatgcgca gggcacccgc ttcctgcagc tcctcgcacg tgtctagccc ggtgatcacg
     5161 aactgcatga agcgctgtgg cagggcgaag aactcgcgca tcatgcgaaa gcccgggaag
     5221 gcgcgccgtc ccgccgtgag catcggctcg tgctccatcg ggcgacgcac gcgcaaggcc
     5281 actttcttct cgatcacgga ttcctgtccg cccttggccg caatcgcgac gaagcgcggc
     5341 ggcgccggca tgttggcgag gcagcggcgg acggtgcagg tgtgcgcgag gtcgctccaa
     5401 aagagcagga tcggcagctc ggccggcatg ggatcgggcg cgaccagcgg cgttccgttg
     5461 cgcgaggaga gagtaatacg caaggccgtg cgatcgaccg acgacgtctc gcccagctcg
     5521 accgcgctg
//
LOCUS       DASPGK010000113         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_264928, whole genome shotgun sequence.
ACCESSION   DASPGK010000113 DASPGK010000000
VERSION     DASPGK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264928"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VEC56_03225"
     CDS             complement(<1..324)
                     /locus_tag="VEC56_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13196.1"
                     /translation="MKQYLLDTWAFNDRANKQVLARIRDLPDTAESIRFFSHLINSQK
                     KWLARIHQFPKDPNLDWWEPGYALEDLERAWDASLAAWREFVADKSEQELFAEVKWVG
                     YDGKTW"
     gene            complement(530..973)
                     /locus_tag="VEC56_03230"
     CDS             complement(530..973)
                     /locus_tag="VEC56_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13197.1"
                     /translation="MNRWIRLCSVTLVLVATATPSFAALGLQAGAAIDPDMFLIGGRF
                     RSSPLSDTSITMVPSIEFGIGDNIWMFAGNLDGHYDFKTDSKYAPYVGAGLTLNWFDF
                     DEGDGDLEFGGSILGGIQLNEKFFFETKVGLGDVPDWKFILGWGK"
     gene            complement(1121..2299)
                     /locus_tag="VEC56_03235"
     CDS             complement(1121..2299)
                     /locus_tag="VEC56_03235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXV13198.1"
                     /translation="MRDAYIISAVRTPVGRAFKGTLRNTRPDELAAIAIREAVARAKG
                     LDPARIEDVVIGCAMPEGEQGLNVARLALLRAGLPITAAGVTVNRFCASGLQAIHDAS
                     ARIRMGEIDVAVAGGTESMSMIPMSGVKPSPNPGLVGDMPGAYVSMGNTAENVAEKYQ
                     IAREAQDAFALRSHTNAAKAIAEGRFNDEIVPVKVVEKSINAKNKIESKEIEFKVDEG
                     VRTDTSLEALAALKPAFRAGGTVTAGNSSQMSDGAAALVIVSEDVMKELGAKPLGKLV
                     AFATAGVEPELMGIGPALAIPKVLKQANLTVGDIGLVELNEAFASQAVYVQQTVGIRS
                     DRLNVNGGAIALGHPLGCTGSKLTTTLLYEMQRRGVEYGIVTMCVGGGMGVAGIFQRA
                     "
     gene            complement(2301..>2679)
                     /locus_tag="VEC56_03240"
     CDS             complement(2301..>2679)
                     /locus_tag="VEC56_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXV13199.1"
                     /translation="DGDSFVMNADRLLSAAKTYALALARRGYQPPDPMVEIPVAGEGG
                     IAAIKTQLYNMKEGRAISEYDAYLGGELARILCGGELPAGTKVSEDYLLQLEREVFLR
                     VCGQKKTQERMAFMLKEGKPLRN"
BASE COUNT          463 a          893 c          817 g          506 t
ORIGIN      
        1 ccaggtcttt ccatcgtagc ccacccactt cacctcggca aacaactctt gctcgctctt
       61 gtcggcgacg aactccctcc acgccgcgag gctcgcgtcc cacgcgcgct cgagatcctc
      121 cagcgcgtag ccgggctccc accaatccag gttgggatcc ttcgggaatt gatggatgcg
      181 cgccagccac ttcttctggg aattgatgag gtggctgaag aagcgaatgc tctccgctgt
      241 gtcgggcaga tcgcggatgc gggccagcac ctgcttgttg gcgcgatcgt tgaacgccca
      301 ggtgtcgagt agatactgct tcatgtccat gccgatcaca ctctctgaaa cataccggcc
      361 acgcccatgc cgccgcccac gcacattcgg aaatcgatgc tggtgctggt ctaaacgtaa
      421 gacttggggg caaccggggt caacgacgca aacatcgtac cggaaagagg cgcgggcggt
      481 gcgcccaacg cgccaccgcc cgcttacaaa ccgcgatatc gatccggcgc tattttcccc
      541 aaccgaggat gaacttccag tcggggacat cgcccagtcc aaccttcgtc tcgaagaaga
      601 acttctcgtt gagttggatg cccccaagga tgctgccgcc gaactcgaga tcgccgtcgc
      661 cctcgtcgaa gtcgaaccag ttcagcgtca ggccggcgcc gacatacggc gcgtacttgg
      721 agtcggtctt gaagtcgtag tgtccgtcga ggttgcccgc gaacatccag atgttatcac
      781 caatgccgaa ttctatactg ggaaccatgg tgatcgacgt gtcgctcagg gggctactcc
      841 gaaagcgtcc gccgatcagg aacatgtcgg ggtcgatcgc cgcgcccgcc tgcaggccca
      901 gagccgcaaa cgacggcgtt gccgtggcaa cgaggaccag agtaacgcta caaagacgta
      961 tccagcgatt catacgcact cctcggtgag gtggcaacgt gccacgcggt taatgaatta
     1021 cactaactgc gcgtgcgaat gaatcgaaga ccggagttgt cgagtcgacg cgcgtgggct
     1081 ggaccatacc acgagcggag gggaaatggg agcgggttcc tcacgctcgc tgaaagattc
     1141 ccgcgacacc catgccgccg ccgacgcaca tcgtcacgat gccgtactcg acgccgcgcc
     1201 gctgcatctc gtagagcagc gtggtcgtga gcttcgaccc cgtgcacccc agcggatgcc
     1261 cgagtgcgat ggcgccgccg ttgacgttca ggcggtcgga gcgaatcccg accgtttgct
     1321 gcacatagac ggcctgcgac gcaaaggctt cgttcagctc gacgagcccg atgtcgccca
     1381 cggtgaggtt cgcttgcttg agcaccttgg ggatggcgag cgcgggcccg atgcccatca
     1441 gctccggctc gacaccagcg gtcgcgaagg cgacgagttt gccgagcggc ttcgcaccga
     1501 gctctttcat gacgtcttcg gagacgatga cgagtgcggc ggcgccgtcg ctcatctgcg
     1561 acgaattgcc ggccgtcacg gtgccgccgg cgcggaacgc gggcttcaac gccgccagcg
     1621 cttcgaggga tgtgtccgtc cgcacgccct cgtcgacctt gaactcgatc tccttcgact
     1681 cgatcttgtt cttggcgttg attgacttct cgaccacctt gaccggcacg atttcgtcgt
     1741 tgaaacggcc ctcggcgatg gccttggcgg cgttggtgtg gctgcgcagc gcaaaggcgt
     1801 cctgcgcctc gcgcgcgatc tggtacttct ctgcgacgtt ctccgcggtg ttccccatgc
     1861 tgacgtaggc gcccggcatg tcgccgacca agcccgggtt gggcgacggt ttcacgccgc
     1921 tcatcgggat catgctcatc gactcggtgc cgcccgcgac ggcgacgtcg atttcgccca
     1981 tgcgaatgcg cgcgctggcg tcgtggatgg cctgcagtcc cgaagcgcag aagcgattca
     2041 cggtgacgcc cgcggcggtg atgggcagcc ccgcgcgcaa gagcgccaag cgcgccacgt
     2101 tcaggccctg ctcgccttcc ggcatcgcac agccgatcac gacgtcttcg atccgcgcgg
     2161 gatccaaccc cttcgcgcgc gcgacggctt cgcgaatggc gatcgccgcc agctcgtcgg
     2221 ggcgcgtgtt gcgcagcgtt cccttgaacg cgcggcccac gggcgtgcgc acggcggata
     2281 tgatgtaggc gtctctcatg ctagttcctc agcggcttgc cttccttcaa catgaacgcc
     2341 atgcgctcct gggttttctt ctgaccgcac acccgcaaga aaacctctcg ctccagttgg
     2401 agcaagtagt cctcgctgac cttggtgccc gccggcagtt ccccgccgca caggatgcgc
     2461 gcgagctcgc cgcccaggta ggcgtcgtac tcgctgatcg cgcgtccttc cttcatgttg
     2521 tagagctgcg tcttgatggc cgcgatgccg ccctcgcccg cgaccggaat ctccaccatc
     2581 gggtcgggcg gttgatagcc gcggcgcgcg agtgccagcg cgtacgtctt cgcggcgctg
     2641 agcaatcgat ccgcgttcat cacgaacgaa tcgccgtcg
//
LOCUS       DASPGK010000114         8721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_966653, whole genome shotgun sequence.
ACCESSION   DASPGK010000114 DASPGK010000000
VERSION     DASPGK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966653"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEC56_03245"
     CDS             <1..249
                     /locus_tag="VEC56_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13200.1"
                     /translation="TVVDEEGVEHQLSGEAVETSVKVYNVTGRLVTTLVESPHAPGRY
                     RTGWSAQDDQGASVASGVYYVKLTIGRRSVTQRMVQLK"
     gene            complement(251..1153)
                     /locus_tag="VEC56_03250"
     CDS             complement(251..1153)
                     /locus_tag="VEC56_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV13201.1"
                     /translation="MSSRLYTATLLFAGIHTIVAPAFAQRIGVYLDPTGDLSSWCNTI
                     PSFQIYVVFHGDPTQYGGGEYKQVRFAVSPPPCASVVADAWVLGLGNVDPSVLLSGVT
                     VPLGSCRSDEAVVARIAVLALGPHTECCALRVVPDSGAPSGMIEFTRCDDEIVRLFEG
                     TVAGNLGDPGQPCTPGTWTLPSNPYPPDGATGVALDTDVAAIVHWPPMVLGCPALYGE
                     MIRVFFGTDPNPPEIGAELLATVAFNPGPLQSNTKYYWKIVYINTLGSGSQLPGEPAV
                     WSFTTTETVATQSSTWGAVKAMYR"
     gene            1347..2678
                     /gene="hflX"
                     /locus_tag="VEC56_03255"
     CDS             1347..2678
                     /gene="hflX"
                     /locus_tag="VEC56_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXV13202.1"
                     /translation="MKRTFELITRTRVERAYLVGVSLPGSSVGAAHEHLDELEQLATT
                     AGAVVVGRTVQGRARVDVTTFIGDGKVDDLKQTCDELGVNLVVFDDDLAPAQAKNLEK
                     RLNVRCIDRTELILDIFARHAKTQQSKIQVELAQLEYSLPRLKHFWQHLERQAGGIGT
                     RGPGETQLEVDRRKIYQRIASLKRQLEKIDRHRETMRNARSSRHVAALIGYTNAGKST
                     VMRRLTGADVLVRDQLFATIDTTTRKMSSNGNGNGNGNGNGNGHASHDILLIDTVGFI
                     RKLPNHLTTSFKATLEDTAQAELYLHVVDVSHPAWEEQMEVTDTTVRSIDNPGVQTLY
                     VFNKIDRMAPPLLEGLRQRFPDAAFVSASEGTGIDELRERIDSFFYGRNIRVEVKLSA
                     GDGKTISLVRGLLHDASNTYEDDLCVLNGTIEAKLMGRLEAIPGTNVRYLI"
     gene            complement(2680..3978)
                     /locus_tag="VEC56_03260"
     CDS             complement(2680..3978)
                     /locus_tag="VEC56_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13203.1"
                     /translation="MPTQPDPTPPSRRQDVAFFVLLNLVGLLFFSLVRNNSFWHGDDW
                     EYLIHALRIEGDWREIFSTRLHQTFQPLPNLIFFLEFQAFQLDAERYYLFNVLVHSAN
                     AFLVYKLVRTLLRDRTIAVLSGLLFEFAVGNYGKSVMTVSGVSDLVITALTLLTMIFY
                     VRNELQDRGALWTRNFFLCLVFFTLSLLSKTTSFSVIGLMFAFNLLYREQTGRRLLDA
                     NFVAIAGYALVVLVVKLVAQGAIPGSQDLAIFSWSFFKNYPAYLVRMVFPIHASTLVS
                     DAGWLVRFIYEFATAIRILIFLSIVSYSVFGFIFGNRVIRFFIAWTYIMITPFCFFRF
                     PSDWLDIRFLYLVSVGFHMILASGTVLAARLLYQKPWRRRLPYLIPAFFVGLSYFVLQ
                     QLDSNYERKAASPQLNTVKGRFYEMYAKKTETRETLPKTE"
     gene            4150..4962
                     /locus_tag="VEC56_03265"
     CDS             4150..4962
                     /locus_tag="VEC56_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HXV13204.1"
                     /translation="MSPLRFGAADFIANRPLLCGLSNGHADLARLTYESQATLVDSFE
                     RGRYDAALVPSIEYLRGAGRYLLPGPALVGRSAPGGIVLVSQKPLEEVQRIAVGECCR
                     TPVAVLRIVLAEQQHVFPDLMVEKRLNEDDWRDRYDAALITGVAALREAVAPPTEGLT
                     RFNVTEMWKSLTKTPLVHSVWVSDEEPRLEEIRNTLASLSTYGLERLASLAEEIALAL
                     SMDAMVAYDYLTRSWSYELGAAEMDGLRALSDYARKYDLVRSSRFATIPAVQ"
     gene            5045..5395
                     /locus_tag="VEC56_03270"
     CDS             5045..5395
                     /locus_tag="VEC56_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXV13205.1"
                     /translation="MSDIVREFNEYRKKMNDRVLATDNLVVKRFFNLDTNTYQGGALP
                     AKTKEMLGLVASMVLRCDDCIAYHVQECHQAGVTREEFFEVFSIGLVVGGSITIPHLR
                     RAVEFLDALESKSK"
     gene            5401..6390
                     /locus_tag="VEC56_03275"
     CDS             5401..6390
                     /locus_tag="VEC56_03275"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HXV13206.1"
                     /translation="MWNRIREFIDRHDAFVVTSHINPDGDAIGSEMGLTHFLRQIGKK
                     VIVMNSTDTPEVLRFLDADNEIRVYGEPGTQPLLDTVDACIIVDVNNWEQLGNIGRAL
                     QARSLPRACIDHHQGNTSGFADHTVTDTSAAATGLLIYEMIRARDGEVTRPIADSLYA
                     AIITDTGTFRFSNTDARVLRTAAELCDAGVVPFEMHRKVFGSKSWGAGRLLGPVLSTL
                     ESEAGGKIAIFTATLEMVNNAGATYDDMDGFVDLVRAIKGVELVLFFKETEEGDIKVS
                     LRSNGKVDAHAIAKQFGGGGHTMASGMRVAGPMQRAIDTVVSSCRNLSEFMIR"
     gene            complement(6395..8422)
                     /locus_tag="VEC56_03280"
     CDS             complement(6395..8422)
                     /locus_tag="VEC56_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13207.1"
                     /translation="MTRSLMRMDLSLNKAPYRFWVAMAVFLLSWIPWAPARVIAIFLA
                     AGLLPGYAIAVRSGVRFDVAVGVGAAISPVIFGAAALVAMLVGADVRVAGTFAKGIGL
                     ILFLALGRPSQPLTNEDRRALAGALVVCVVAAVLALSLPLAETWWRVREDSWFHAAVA
                     DKLARDGLPLSDPYFAGLRIQYMYVYHAIVAACSSLCRIDYFHAMILLNAMALSSTVF
                     AFHALAGEFTRRAGPRVLGTFFWVFAMNGWFYLSYPVRLARAVFGETHGLEALRAMFP
                     WTPPGHATAMSLLSVEGNQFMFLDKFMIGTALSLTFGLAATLLLLLVRARRGQWSAYD
                     DVAFVLALAGSVLLHLVTGLTVAFVTAAVLAFLLVIRSQPSPGGPSYARLVGWIVLVL
                     AATAPYLYSVAPREGSASIAFALQRAHAVGLVFDVLPALVLAVVFLRSASRDTADALG
                     ARPLAEMSLSATGILLLWTLLVAGVGLTFDLATNNETKFAFLVFLPLAALAVGALDRW
                     WDARCSRVVAIVLVASATLPLHAIYFAHAFRDASTFDVSDPEKAVYGWVAKSAPRNAI
                     VIEENDIVRIPVLASRDLYWGTEGYARNWGYPQDEMIARKRVRDAVFSDAGASELDMM
                     RLRSLERPVLVVYRRHPEGMIDAPERFEKDRRFHGRFATPEIAVWELFVDE"
BASE COUNT         1778 a         2792 c         2822 g         1329 t
ORIGIN      
        1 accgtggtcg acgaagaggg cgtcgagcac caactctccg gcgaagccgt cgagaccagc
       61 gtcaaggtgt acaacgtcac cggtcgcctg gtcacgaccc tggtcgaatc cccgcacgcg
      121 cccgggcgct atcgcacggg ctggtcggcc caagacgacc agggcgcgag tgtggcgagc
      181 ggggtctact acgtgaaact caccatcggc cggcgctcgg tcacgcaacg catggtgcag
      241 ttgaagtagc tcaccgatac atcgccttta cggcccccca cgtgctcgac tgggttgcca
      301 cggtttccgt cgtggtgaag ctccatacgg ccggttcacc cgggagctgg gagccactac
      361 ccagggtgtt tatgtagacg atcttccagt agtatttcgt gttcgactgc agcgggcccg
      421 ggttgaacgc gaccgttgct agaagttcgg cgccgatctc gggcggattc ggatcggtac
      481 cgaagaacac tcggatcatc tcgccataaa gcgcggggca accgagcacc atgggcggcc
      541 agtggacgat tgcggccacg tcggtatcga gcgcaacgcc agtggcgcca tccggcgggt
      601 aggggttgga tgggagcgtc caggtaccgg gggtgcacgg ctgacctggg tcgccgagat
      661 tgcccgcgac ggttccttcg aacaaacgca cgatctcgtc atcgcagcgc gtgaactcga
      721 tcatgccgga gggcgcaccc gagtccggca ctactcggag ggcgcagcat tccgtgtgag
      781 gacccagcgc gagtaccgct atccgggcca cgaccgcctc gtccgatcga cacgacccaa
      841 gaggaaccgt cactccgctc aggagcacgc tggggtccac atttccgagc cccaacaccc
      901 aggcgtcggc aacgacgctg gcgcacggtg gcggagagac ggcgaatcgg acttgcttgt
      961 attcgccgcc gccgtactga gtgggatcac cgtggaatac gacatagatc tggaaggacg
     1021 ggatggtgtt gcaccatgag gagaggtcgc ccgttgggtc caggtagacg ccgattctct
     1081 gcgcaaacgc gggcgcaacg atagtgtgga tgcccgcgaa caagagcgtt gcggtatata
     1141 gcctggagga catactccgc ctgccggacg agggggaaat gcgcgacgta gccgaccaac
     1201 caatgctaat cgagatacgc gagtggaatc aatcccgccg ctatcgactt ggccccaatt
     1261 ttgttatact ccgggcagat ttgacccatt agcgaggttg ccatcgaaac cagacgtctt
     1321 tcacaaagcc gaggttgcct tcccaagtga agcgcacgtt tgaacttata actcgaacgc
     1381 gcgtcgagag ggcctacctc gtcggcgtga gtctgccggg gagttccgtc ggcgccgcgc
     1441 acgagcacct ggacgagctc gagcagcttg caaccaccgc gggcgccgta gtcgtcggtc
     1501 gcaccgtcca aggacgcgcc cgcgtcgacg tcaccacctt catcggcgac ggtaaagtcg
     1561 acgacttgaa gcagacctgc gacgagctcg gcgtcaacct ggtcgtgttc gacgacgacc
     1621 tcgcacccgc gcaggccaag aacctcgaaa agcgtctcaa cgttcgctgc atcgaccgca
     1681 ccgagctgat cctcgacatc ttcgcgcgcc acgcgaagac ccagcagtcc aagatccaag
     1741 tcgagctcgc gcagctggag tattcgctgc cgcgcctgaa gcatttctgg cagcacctcg
     1801 aacggcaggc gggcggtatc ggaacacgcg gccccggcga aacccagctc gaagtcgacc
     1861 gccggaagat ctatcagcgc atcgcgtcgc tgaagcgcca gctcgagaag atcgaccgcc
     1921 accgcgagac catgcgcaac gcgcgctcga gtcgtcacgt cgccgccttg atcggctaca
     1981 ccaacgcggg caagtcgacc gtgatgcggc gattgaccgg cgccgacgtc ctggtgcgcg
     2041 accagctctt cgcgaccatc gacaccacga cgcgcaagat gagctccaac ggcaacggca
     2101 atggaaacgg gaatggtaac ggcaacggcc acgcgagcca cgatattctg ctgatcgaca
     2161 ccgtcgggtt catccgcaag ctgcccaatc acctgacgac gtccttcaag gccaccctcg
     2221 aagacaccgc gcaggccgag ttgtacctcc acgtcgtcga cgtgtcgcac cccgcgtggg
     2281 aagagcagat ggaagtgacc gacacgaccg tgcgctcgat cgataatcct ggggtgcaga
     2341 cgctctacgt gttcaacaag atcgatcgca tggcgccgcc cctcttggaa ggacttcgcc
     2401 agcgcttccc cgacgcggcc ttcgtgtcgg cctccgaagg caccggcatc gacgagctgc
     2461 gcgagcgcat cgactcattc ttctatggtc gtaatattcg ggtcgaagtg aagctctcgg
     2521 ccggcgacgg caagaccatc tcactcgtgc gcgggctcct gcacgacgcg agcaacacct
     2581 acgaagacga cctctgcgtg ttgaacggca ccatcgaagc caagctgatg ggccgcctgg
     2641 aagcgattcc ggggaccaac gtccgctact tgatctagct cactccgtct tcggtagcgt
     2701 ctcgcgcgtc tccgtcttct tcgcgtacat ctcgtagaac ctccccttga ccgtgttcag
     2761 ctggggcgag gccgccttgc gctcgtagtt cgagtcgagc tgctgcagca cgaagtacga
     2821 gagccccacg aagaacgcgg ggatcagata cggcaatcgg cgtcgccacg gcttctgata
     2881 cagcagccgc gccgcgagta ccgtccccga cgcgaggatc atgtggaagc cgaccgacac
     2941 cagatacagg aaccgaatgt cgagccaatc cgatggaaac cgaaagaagc aaaacggcgt
     3001 gatcatgatg taggtccaag cgatgaagaa gcggatcacg cggttgccga aaatgaaccc
     3061 gaagaccgag tacgagacga tgctcaagaa gatgaggatc cgaatcgcgg tcgcgaactc
     3121 gtagatgaag cggaccagcc agccggcgtc ggagaccagg gtggaggcgt ggatcgggaa
     3181 caccatgcgg accaggtacg cggggtagtt cttgaagaac gaccacgaga agatggccag
     3241 atcctgcgag ccggggatcg ccccctgggc gacgagcttg accaccagga cgaccagcgc
     3301 atagccggcg atggccacga agttcgcgtc caggagcctt cggccggtct gctcccgata
     3361 gagcaggttg aaggcgaaca tgagcccgat gaccgagaac gaggtcgtct tggaaaggag
     3421 gcttagcgtg aaaaagacca agcaaagaaa gaagttacgt gtccacaggg ccccccggtc
     3481 ctggagctcg ttgcgaacgt agaagatcat cgtgaggagg gtgagcgccg tgatgacgag
     3541 gtcgctcacc ccggagaccg tcatcaccga cttcccgtag ttccctaccg cgaactcgaa
     3601 caggagcccc gacagcaccg cgatggtccg gtccctcagg agggtccgga ccagcttgta
     3661 gaccaggaac gcgtttgcgg aatgcacgag gacgttgaac aggtagtagc gttccgcatc
     3721 gagttggaag gcctggaact cgaggaagaa gatcaggttg ggcagcggct ggaacgtttg
     3781 gtggagccgg gtcgaaaaga tctcccgcca atccccctcg atccgaaggg cgtggatcag
     3841 atactcccaa tcatcgccgt gccagaagga attgttacgg acaagagaga agaataggag
     3901 cccgaccagg ttcaggagga cgaagaaagc gacgtcctga cggcgggacg gaggggtcgg
     3961 gtcgggttgg gtcggcatcc gggaatcctc gggagacgag gcgagcagca tcataaccca
     4021 agtggaataa cgcttgctgt aatttttgag cgggtgctat aagcagcgca ccttcgaccg
     4081 ccgcgggcgt ggtgctccgc gcgccgaagg gcaaagacga ccaccgaacc cgtcccgtgg
     4141 gaggacgacg tgagcccgct ccgattcggc gccgccgact tcatcgccaa ccggcctctc
     4201 ctgtgcgggc tctccaacgg acacgccgac cttgcgcgcc tgacctacga gtcccaagcc
     4261 accctcgtcg attcgttcga acgcggccgc tacgacgcgg ccctggtccc gagcatcgag
     4321 tacctgcgcg gcgcggggcg ttacctcctc ccgggccccg cgctggtggg tcgctcggcg
     4381 ccgggcggca tcgtgctggt ctcgcagaag ccgctcgaag aggtccagcg catcgccgtg
     4441 ggcgagtgct gccggacacc ggtggcggtg ctccgcatcg tattggccga acagcagcac
     4501 gtcttccccg acctcatggt cgagaagcgc ctgaacgagg acgattggcg cgaccgttac
     4561 gacgcggcgt tgatcaccgg cgtggcggcc ctgcgcgaag ccgtcgcccc gccgaccgaa
     4621 gggctcacgc gcttcaacgt caccgagatg tggaaatcgc tcaccaagac cccgctcgtg
     4681 cactcggtgt gggtgagcga cgaagaaccg cgcctcgaag agatccgcaa cacgctcgcg
     4741 tcgctgagca cgtacggcct cgagcgactg gcgtcgctgg ccgaagaaat cgccctcgcg
     4801 ctctcgatgg acgcgatggt cgcctacgac tacctcacgc gctcgtggtc ctacgagctc
     4861 ggcgccgccg agatggacgg gctgcgcgcg ctctcggact acgcccgcaa gtacgacttg
     4921 gtgcgcagca gtcgattcgc gaccatccct gcggtccagt aaccgctttc acgcccgtca
     4981 acgagccgat ggcgcgaagc gcctcggtag gctattctgt tcggacattg cgccggaggc
     5041 aggtatgtcc gacatcgtcc gcgagttcaa cgagtaccgt aaaaaaatga acgatcgcgt
     5101 cctcgcgacc gacaatctgg tcgtgaagcg gttcttcaac ctcgacacca acacgtatca
     5161 ggggggcgca ctcccggcca agaccaagga gatgctgggg ctggtcgcct cgatggtgct
     5221 gcgctgcgac gactgcatcg cctaccacgt ccaggagtgc caccaggccg gtgtcacgcg
     5281 cgaggagttc ttcgaggtat tcagtattgg gctggtcgtg ggcgggtcga tcacgattcc
     5341 gcacctacgc cgcgcggtcg aattcctcga cgccctggag tcaaagtcga agtagccgcc
     5401 atgtggaacc ggatccgcga gttcatcgac cgccacgacg ccttcgtggt gacgtcccac
     5461 atcaacccgg acggcgacgc catcggctcg gagatggggc tcacgcattt tctccgccag
     5521 atcgggaaga aagtgatcgt gatgaactcc accgacacac ccgaggtgct tcgcttcctc
     5581 gacgccgaca acgagattcg cgtctacggc gagccgggga cgcaaccgct cctcgacacg
     5641 gtcgatgcgt gcatcatcgt cgacgtcaac aactgggagc agctcggtaa catcggccgc
     5701 gcgctccaag cgcgttcgct accgcgcgcg tgcatcgacc accaccaggg gaacacgtcc
     5761 ggcttcgcgg atcacacggt gaccgacacc tcggccgccg ccaccggcct cttgatttac
     5821 gagatgattc gggcccgcga cggcgaggtg acgcgcccca tcgccgactc gctctacgcc
     5881 gccatcatca ccgacaccgg cacgttccgc ttctcgaata cggacgcgcg cgtgctgcgc
     5941 acggcggcgg agctgtgcga cgcgggcgtg gttccgttcg agatgcaccg caaggtgttc
     6001 gggagcaaga gctggggggc ggggcgtctg ttggggccgg tcttgagcac gctggagtcc
     6061 gaagcgggcg ggaagatcgc catattcacg gccacgctcg agatggtcaa caacgcgggc
     6121 gccacctacg acgacatgga cggtttcgtc gacctggtac gcgcgatcaa gggcgtcgag
     6181 ctggtgctct tcttcaagga gaccgaggaa ggcgacatca aggtgtcgct gcgctccaac
     6241 ggcaaggtcg acgcgcacgc gatcgcgaag cagtttggcg gcggcgggca cacgatggcg
     6301 tcgggcatgc gcgtcgcggg gccgatgcag cgcgccatcg acacggtggt gtcttcgtgc
     6361 cgcaatctct ccgagttcat gatccgctaa cgcctcactc gtccacgaac agttcccaca
     6421 ccgcgatctc cggggtcgcg aagcgcccgt ggaagcgccg gtccttctcg aagcgctcgg
     6481 gcgcgtcgat catgccctcc ggatgccgac gatacacgac caagacgggc ctctcgagcg
     6541 agcgcaggcg catcatgtcg agctcgctcg cgcccgcgtc ggagaacacg gcgtcgcgca
     6601 cgcgcttgcg cgcgatcatc tcgtcctgcg ggtagcccca gttgcgcgcg tagccttccg
     6661 tgccccagta caggtcgcgc gaggccagca cgggaatgcg cacgatatcg ttctcttcga
     6721 tgacgattgc attgcgcggt gcgctcttgg cgacccaacc gtagaccgcc ttttcgggat
     6781 cgctcacgtc gaaggtggag gcgtcgcgga aggcgtgcgc gaaatagatc gcgtgcagag
     6841 gaagtgtcgc ggacgcgacg agcacgatgg cgacgacacg cgagcatcgt gcatcccacc
     6901 accgatcgag cgcacccacc gcgagcgccg cgagcggcag gaacacgaga aacgcgaact
     6961 tggtctcgtt gttggtggcg aggtcgaagg tgaggccgac gcccgcgacc agaagcgtcc
     7021 acagcagcag aatcccggtc gcgctcaagc tcatctcggc caagggccga gcgccgagcg
     7081 cgtcggccgt gtcgcgcgac gcgctgcgaa ggaacacgac ggccaggacc agcgccggaa
     7141 gcacgtcgaa gaccagtccg accgcgtgtg ctcgctggag cgcgaaggcg atcgatgcgc
     7201 tcccctcgcg cggcgcgacc gagtacaggt agggcgcggt ggccgcgagt accaacacga
     7261 tccatcccac caggcgcgcg tacgacggcc cgcccggcga cggttgcgaa cgaatcacca
     7321 gcaggaacgc gagcacggct gccgtcacga aggccacggt caatccggtc acgaggtgca
     7381 acaacaccga gcccgcgagt gcgaggacga aggcgacgtc gtcgtacgcg ctccactgcc
     7441 cgcgccgcgc gcgcacgagg agcaagagca gcgtcgccgc gagcccgaag gtcaacgaca
     7501 gcgcggtgcc gatcatgaac ttgtcgagga acatgaactg gttgccttcg accgacaaga
     7561 gggacatcgc ggtcgcgtga ccgggcgggg tccacgggaa catggcgcgc agcgcctcca
     7621 gcccatgcgt ctcgccgaag accgcgcgcg cgaggcgcac cggatacgac aggtagaacc
     7681 agccgttcat cgcgaacacc caaaagaagg tgccgaggac gcgcgggccc gcacggcgcg
     7741 tgaactcacc cgcgagcgcg tggaacgcga acaccgtgga cgagagcgcc atcgcgttca
     7801 gcaggatcat ggcgtggaag tagtcgatgc ggcaaagcga cgagcacgcc gcgactatcg
     7861 cgtgatagac gtacatgtac tggatgcgaa gccccgcgaa gtacggatcg gagagcggaa
     7921 gtccgtcgcg cgcgagcttg tcggcgaccg cggcgtggaa ccagctgtcc tcgcgcacgc
     7981 gccaccacgt ttcggcgagc ggaagcgaga gcgcgagcac ggcggcgacg acgcaaacga
     8041 cgagcgcgcc ggccagcgcg cgacgatctt cgtttgtgag cggttgcgag ggacgaccga
     8101 gcgccaagaa cagaatgagc ccgattccct tcgcgaatgt tccggccacg cgcacgtcgg
     8161 caccgaccag cattgcgacg agcgccgccg cgccgaagat cacgggcgag atcgccgcgc
     8221 ccactcccac cgcgacgtcg aaccgcacgc ccgagcgaac cgcaatcgcg tagccgggca
     8281 ggagtccggc cgcgaggaag atcgcgatga cgcgcgccgg agcccaggga atccacgaca
     8341 gcagaaagac cgccatcgcg acccaaaagc gatacggcgc cttattgagc gagagatcca
     8401 tccgcatgag tgagcgggtc acgctgcgca catacaggcg tgtgaggtgg gcgcgtccgc
     8461 gaggtgtaac acgctgcgaa gtgccatgtt accgcaaatg agacggccct ggaattgcaa
     8521 gcgcggggag cgcaagggtc tcaatcccag ggccgtgtgg tgatggaggt tgctagtgct
     8581 ctcttctacg agagagcgcg ccgaaagttt cacgccgcgg ggatgcctgc gacctcgcgg
     8641 ggccgcagga ttcgatagat gtgcgcgagc gccagcccgc ttaccgggag cgaaaccagc
     8701 gcgccgatac ccaggcaaat c
//
LOCUS       DASPGK010000115        10566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1084369, whole genome shotgun sequence.
ACCESSION   DASPGK010000115 DASPGK010000000
VERSION     DASPGK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084369"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..450)
                     /locus_tag="VEC56_03285"
     CDS             complement(55..450)
                     /locus_tag="VEC56_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HXV13208.1"
                     /translation="MGVVAGLAAQTLKLFSFLILEKRINFRRLVETDGAPNMHSAAFA
                     GLTTAIAQSDGLATLEFALAASLTTLITVDIWNVKHAASRQADVVDSIVRRIRPELAG
                     AERRSLSYTVFDVLAGTGVGVALAWIIPK"
     gene            complement(494..1408)
                     /locus_tag="VEC56_03290"
     CDS             complement(494..1408)
                     /locus_tag="VEC56_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXV13209.1"
                     /translation="MTPGDFESALARDRDLVEARLRAIVEERASHHAGIAEAIRYALL
                     GGGKRLRPLLCLWTHDVFAGPARARTRATALDAACAVECVHAYSLAHDDLPCMDDDDL
                     RRGKPSLHRRFDEATAVLTGDALLTLAFDVLANLDAAPAVALDAVRVLASAAGTGGLV
                     TGQALDLEHTGRDDDRALALVERIHEFKTARLFAAALEIGAVAAWDGRVPDGDARERV
                     RRAGHQAGSAFQIVDDLLDIEGDAATLGKTPRKDVARGKLTFPAVAGRGAAGQAAHDR
                     IASALGTLPEAGGTPLASLIALVETRRS"
     gene            complement(1405..1683)
                     /locus_tag="VEC56_03295"
     CDS             complement(1405..1683)
                     /locus_tag="VEC56_03295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXV13210.1"
                     /translation="MKEKPNPIPPAGDDAPSFEEAMTRLERIVADLEGGRYTLEESLQ
                     RFEEGIALGNQCRRMLERADLRVRTLVEAGDRLVVSDPTETPDEEPRA"
     gene            complement(1680..2909)
                     /gene="xseA"
                     /locus_tag="VEC56_03300"
     CDS             complement(1680..2909)
                     /gene="xseA"
                     /locus_tag="VEC56_03300"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXV13211.1"
                     /translation="MVHGESTVFAVSELNALVRELLEDQFAEVAVEGETSNVRVHASG
                     HVYFRLKDASASLSAVCFRSDARLLDFDLSDGQRVVARGRLTIYEVQGSYQLVARAIE
                     PAGRGDLERAFRLLVSKLDQEGLFDEARKRPLPRYPRTIAVVTSPTGAAVRDIFSTLA
                     RRFPCVEVVFAPVQVQGERAPFEIVRALDALNDHDVDVVILGRGGGSIEDLWAFNDES
                     VARAIHRSRAPVVSAVGHETDVTIADFVADRRAATPTMAAEIVVPEREDVARRLDAYA
                     EKSRARMTARVGLEKRRVAELVRSYALGQVRGRVERGMQSLDFALERVQRALVSAALA
                     RRAALDTAGARLAALDPREVLRRGYAVCHDESGAHLVRGAHEAAAAGRMRVTFHDGVV
                     AAAVEGMEIPSAKEAAS"
     gene            complement(2913..3788)
                     /gene="folD"
                     /locus_tag="VEC56_03305"
     CDS             complement(2913..3788)
                     /gene="folD"
                     /locus_tag="VEC56_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXV13212.1"
                     /translation="MQLLDGKACAAEIKREVATETARLARARKPVLALVRVGEDPASK
                     VYVASKAKASEECGIESRNVHLPEDSSEARVLGVLSEFNEDRDVDGILLQLPLPRQVD
                     ADRAIASISPEKDVDGFHPESLGRLAAGAPRFVPCTPLGIRELLIRNQIATAGANVVV
                     LGRSVIVGKPMALLLSLKGEGGDATVTMCHSRSRDVAAHTRRADIVIAAMGVPRFLQG
                     DMIKDGAVVVDVGINRVDDPSSPKGYHLVGDVDFDAVAPRTSFITPVPGGVGPMTVAM
                     LMANTLSAFRMRTGS"
     gene            complement(3788..4567)
                     /locus_tag="VEC56_03310"
     CDS             complement(3788..4567)
                     /locus_tag="VEC56_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXV13213.1"
                     /translation="MIIAFLGDVVGKTGRRAVEWCVPRIRERHRTDLLIANAENSAGG
                     FGINPTGIAEMEAAGIAFFTTGNHVWDKREGIALLDSKPNIVRPANYPDAPGRGAALV
                     PGTDGRVAVLNVQGRVFMPPLDCPFRTTDRLLSELPESAKVILVDFHGEATSEKIAMG
                     FYLDGRVSLVLGTHTHVPTKDARILPGGTGYVTDVGMTGSYDSILGVRKEDVIDRFLS
                     MRPTRFEVAKLDVRCDYIVADVDEATGRARRFEHHQLVLED"
     gene            complement(4587..6155)
                     /gene="rny"
                     /locus_tag="VEC56_03315"
     CDS             complement(4587..6155)
                     /gene="rny"
                     /locus_tag="VEC56_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXV13214.1"
                     /translation="MNPNWLWVVLGGVAAAIAGFALGIAGARRVARSGLLRAEQLAEQ
                     MAADSTRRADSIVRAASLEAENAMAAQRIELEREMLGAREDLRRRVHDADVRESALAK
                     RLEILDRKDAGLGERSLALDAKQKVLEERLKESTQLIAEENARLERIAGVRKEDAKRM
                     LLKNLEDEAHVDASRRAREIREEAERWAQQESRKIIALAIQRYAAEQCVETTVAVVGL
                     ASDDMKGRIIGREGRNIRAFEKETGVDVVIDDTPGAVLISSFDPVRREVARLAMEKLV
                     QDGRIHPGRIEEIVAEVRASVDAGVRESGERALLDLGINGMAPALVDCVGRMKFRTSY
                     GQNCLAHSLEVAYLAGVMASELNLDPKVARRAGLLHDIGKVASHEAEGSHTTVGAELA
                     RRHGEPEDVVNAIAAHHGDVPTTSLYTPIVAAADAVSGSRPGARRESIEHYVKRLAKL
                     EEIADAFAGVEKSYAIQAGREIRVVVSGRKVDDEQAKQLAFDISRRLEKEIEYPGQIK
                     VVVIRETRAIEYAK"
     gene            complement(6270..6449)
                     /gene="ssrS"
                     /locus_tag="VEC56_03320"
     ncRNA           complement(6270..6449)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEC56_03320"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6503..6826)
                     /locus_tag="VEC56_03325"
     CDS             complement(6503..6826)
                     /locus_tag="VEC56_03325"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXV13215.1"
                     /translation="MNPDDERVEMENTKATTVEIMGRAYRVRGAADEQYVQEVARYVD
                     EKVREVSQGSSLPASDRVAILAAMNIADELFQLRRASHEEMSTIERKAESLISLLDEK
                     LSVDG"
     gene            complement(6860..7144)
                     /locus_tag="VEC56_03330"
     CDS             complement(6860..7144)
                     /locus_tag="VEC56_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13216.1"
                     /translation="METKPAIEKLSMLEDRIAQTAERFAHLKDQYRALQEQRSSLERE
                     IDKLKSANRELNDRINHLKSNHDKILSSFHKEEVRKRIDRVLEKLGELQL"
     gene            complement(7165..9564)
                     /gene="pheT"
                     /locus_tag="VEC56_03335"
     CDS             complement(7165..9564)
                     /gene="pheT"
                     /locus_tag="VEC56_03335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXV13217.1"
                     /translation="MNVSLSWLRRYVDITVDAPTLAHDLTMHGIKVERLTSSGLTERL
                     VVVGHVLEAKRHPEADRLSVCRVDVGQGEPLEIVCGAPNVASGQRVAVALVGAKLPNG
                     VKLRKSKIRGVASNGMICSEVELGLGTESGGILVLAPDAPVGVPLADVLGETDAVLEL
                     EVTPNRPDQLSHVGVAREIAALYRTRLRPPQVETPVETPDDTEVAVEIADPADCFRFT
                     ARVIHGVVVKPSPAWLKGALERVGVHSVNNIVDVTNFVMMEMGQPMHGYDVNGLPSSR
                     MGVRRARRGESIEALDAATYELDDSHLVITAGDDVVGVAGVIGGMASRINEATTDVLL
                     ESAAFDPKTVRRTRRALGLSTDASYRFERGSDREACRRASDRAAALIVEVAGGRVGKW
                     VDVFPAPRQPRAVTLRRSTVRRLLGENLTSDAIAEILGRLEFALVGSDADAVTVAVPS
                     FRWDIEQEADLVEEVARVYGYENIGKGWRYRSTVPSAPDPFDRFVDRLAEHLVARGHT
                     EILTTSFSDGREQRWFGWADSDPRSQAVVLKNPLSSTHAQMRTHLLPGALDAVAHNVA
                     RGRRELYLFALGRVFVRSAESAALPDEPTQILIARTRPEGSAFWRAGAEPVDFFEIKA
                     EVESLLATHAPDVYADLVYEFEPTHGTFRFSDRRRAVVEGGIVPVAAARELDLTQPVW
                     YAVVDVSALFEHAAATPAFKPYSEYPPSRRDLSLVAPSGIVWAQIEKHVVKVGGRLLE
                     SLQVFDVYRGGGLGEGMTAYGVRLSFRSNDATLTDTDVDALIAKLVSKLESELGVVLR
                     S"
     gene            complement(9578..>10566)
                     /gene="pheS"
                     /locus_tag="VEC56_03340"
     CDS             complement(9578..>10566)
                     /gene="pheS"
                     /locus_tag="VEC56_03340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXV13218.1"
                     /translation="ADVAACSTPDELEAARVKYLGRKGVLTAFLRAIGEARAEDRPRL
                     GAEANRLKALGETLLAERRAELGGADERADYAAPVDGTLPATNGLCGRRHVISQTFRR
                     IGSVLEGMGYRLARGPEVELEYYNFQALNFPDEHPSRDLQDTFYVSNDILLRTHTSPI
                     QVRYMQEHKPPLKVYAPGRVYRNEALDASHQAEFHQVEGLYIDTDVTMADLRGDLLLF
                     LRDLFGSDIQVRFKPHFFPFTEPSVDVDMSCFNCHGGGCVICGQSGWIEILGAGMVHP
                     SVLRAVGIDPDRYSGFAFGVGVDRIAMIRHGIPDIRMFLTNDLRFLDQFAER"
BASE COUNT         1617 a         3615 c         3562 g         1772 t
ORIGIN      
        1 agatccgatg ggtagtggat gcggtcgagg atgctcatgc gatgcgcctt cttgttactt
       61 cgggatgatc caggccagtg ccacccccac accggtgccc gccaggacgt cgaacaccgt
      121 gtacgacaac gaccgtcgct cggccccggc cagttcgggc cggatgcgcc gtacgatcga
      181 atcgacgacg tccgcttggc gcgacgccgc gtgtttgacg ttccagatgt ccaccgtgat
      241 cagggtggtc aggctcgccg ccaacgcgaa ctccaaggtt gccaacccat cgctctgcgc
      301 gatcgccgtg gtcagtccgg cgaacgcggc actgtgcatg ttgggggcgc cgtccgtctc
      361 gacgagccgt cggaagttta tccgcttttc caagatgagg aaagaaaaca gtttgagggt
      421 ctgtgccgcc agcccggcca cgacacccac gaccaagggg cgcgcgatgg gggcatcgaa
      481 tagaccctcc accctacgac cgcctcgttt cgaccaacgc gatgagtgaa gcgagcgggg
      541 tcccgccggc ttcgggcagg gttccgaggg cgctcgcgat gcggtcgtgc gcggcctgcc
      601 cggcggcgcc acggccggcg acagccggga acgtgagctt cccgcgcgcg acgtccttgc
      661 ggggggtctt tccgagggtc gcggcgtcac cctcgatatc gagcaagtcg tcgacgattt
      721 ggaaagcgct gcccgcctgg tgcccggcgc ggcgcacgcg ctcgcgcgcg tcgccgtccg
      781 ggacccgtcc gtcccacgcg gccacggcgc cgatctccag cgccgccgcg aagaggcgtg
      841 cggtcttgaa ctcgtggatg cgctcgacca gcgcgagggc gcgatcgtcg tcacggcccg
      901 tgtgctcgag gtcgagggcc tggccggtga cgagcccgcc ggtgccggcc gccgacgcga
      961 gcacccgcac cgcgtccagg gcgaccgccg gcgcggcgtc caggttcgcg agcacgtcga
     1021 aggcgagggt gagcagcgcg tccccggtca gcaccgcggt cgcttcgtcg aagcggcggt
     1081 ggaggctggg ttttccgcgc cgcagatcgt cgtcgtccat gcacgggaga tcgtcgtggg
     1141 cgagcgaata ggcgtgcacg cactcgacgg cgcaggccgc gtcgagcgcg gtggcgcgtg
     1201 ttcgcgcgcg cgcggggccg gcgaagacgt cgtgcgtcca caggcacagg agcgggcgca
     1261 ggcgctttcc gccccccaag agcgcgtagc gaatcgcctc ggcgataccg gcgtggtgcg
     1321 acgcgcgctc ctcgacgatc gcgcgcaggc gcgcctccac caggtcgcgg tcgcgcgcga
     1381 gcgcggactc gaagtcgcca ggcgtcatgc gcgcggttcc tcgtcgggcg tttcggtcgg
     1441 atcggagacg acgagacggt cgccggcttc gaccagcgtg cgcacgcgca ggtcggcgcg
     1501 ctcgagcatg cgccggcact ggttcccgag cgcgatcccc tcttcgaacc gttgcagcga
     1561 ctcttcgagc gtgtagcggc ctccctccag gtcggccacg atgcgctcga gccgggtcat
     1621 cgcctcttcg aacgacggtg cgtcgtcgcc ggcgggtgga attgggttgg gcttctcttt
     1681 cacgacgctg cctccttggc ggacggaatc tccatgccct cgacggccgc cgccaccaca
     1741 ccgtcgtgaa aggtgacgcg catgcgcccg gccgcggccg cttcgtgcgc gccgcgcacc
     1801 aggtgcgcac cggattcgtc gtggcagacc gcgtagccgc ggcgcagcac ctcgcgcggg
     1861 tcgagtgccg ccaggcgcgc gcccgcggtg tccagcgccg cccgccgcgc gagcgcggcc
     1921 gagaccagcg cgcgctgcac gcgctccagc gcgaaatcga ggctctgcat cccccgctcg
     1981 acacgcccgc gcacttgtcc gagcgcgtag ctgcgcacca gctcggccac gcgccgcttc
     2041 tcgagcccca cgcgcgcggt catgcgcgcg cggcttttct ccgcgtaggc atcgaggcgg
     2101 cgcgccacgt cctcacgctc cggcaccacg atctcggccg ccatggtggg tgtggcggcc
     2161 ctgcggtcgg ccacgaagtc ggcgatggtg acgtcggtct cgtggcccac ggcggagacc
     2221 acgggggcgc gactgcggtg gatggcacgc gccacgcttt cgtcgttgaa ggcccacaag
     2281 tcttctatac tgccgccgcc gcggccgaga atcacgacgt cgacgtcgtg atcgttgagc
     2341 gcgtcgagcg cgcgcacgat ctcgaagggg gcgcgctcgc cctgcacctg caccggcgcg
     2401 aacacgacct cgacgcacgg aaagcgtcgc gccagggtcg agaagatgtc gcgcacggcg
     2461 gcgccggtgg gcgatgtgac caccgcgatg gtgcgcgggt agcgcgggag cggacgcttg
     2521 cgtgcttcgt cgaagaggcc ctcctggtcg agcttggaaa ccaggaggcg aaacgcgcgc
     2581 tccaggtcgc cgcggcccgc gggctcgatg gcgcgcgcca ccaactggta gctgccctgc
     2641 acttcgtaga tggtgaggcg cccgcgcgcg accacgcgct ggccgtcgct caggtcgaaa
     2701 tcgagcagcc gcgcgtcgct gcgaaagcac accgcggaga gcgacgccga cgcgtccttg
     2761 agccggaagt aaacgtggcc ggaggcgtgc acgcgtacgt tggacgtctc gccctccacc
     2821 gcgacttcgg cgaattggtc ctccaggagc tcgcgcacca gggcgttgag ttcggatact
     2881 gcgaataccg tcgactcgcc gtggaccatc ttctagcttc ccgtgcgcat ccgaaacgcg
     2941 gagagcgtgt tcgccatcag catcgccacc gtcatcggcc ccaccccgcc cggcaccggt
     3001 gtaatgaacg acgtgcgcgg tgcgaccgcg tcgaaatcga cgtcgcccac caggtggtac
     3061 cccttgggag aagaggggtc gtccacgcga ttgatcccga cgtcgaccac caccgcgccg
     3121 tccttgatca tgtcgccttg gagaaaccgg gggaccccca tggccgcgat cacgatgtcg
     3181 gccctccgcg tgtgcgcggc gacgtcgcgg cttcggctgt ggcacatcgt gacggtggcg
     3241 tcgccgcctt ctcctttgag cgagagcagg agcgccatcg gcttgcccac gatcacgctg
     3301 cgtcccagca cgaccacatt ggcgcccgcg gtcgcgatct ggttgcgaat caagagctcg
     3361 cggatcccga gcggcgtaca cggcacgaag cgcggggcac ccgccgcaag ccggcccaga
     3421 ctttccgggt ggaagccgtc cacgtccttc tcgggactga tgctcgcgat cgcgcgatcc
     3481 gcatcgacct ggcgcgggag cggcagctgc agcaggatac cgtccacgtc acggtcttcg
     3541 ttgaactcgc tcaggacgcc gagaacgcgc gcctccgagc tgtcttcggg caggtgcacg
     3601 ttgcgcgact cgatcccgca ctcctcgctg gccttggcct tggaagcgac atagaccttg
     3661 gacgcggggt cttcgcccac gcgcaccagc gccagcacgg gcttgcgcgc gcgcgccagc
     3721 cgcgccgtct cggtggccac ttcgcgcttg atctcggccg cgcacgcctt tccgtcgaga
     3781 agctgcatca atcctccagg accagttggt ggtgttcgaa gcggcgcgcg cgcccggtgg
     3841 cctcgtcgac gtcggcgacg atgtaatcgc accgcacgtc cagcttggcc acctcgaaac
     3901 gcgtgggccg catgctcaag aagcgatcga tcacgtcttc tttacgcacg cccaggatcg
     3961 agtcgtacga ccccgtcatg ccgacgtcgg tcacgtagcc ggtgccgccg ggcaagatgc
     4021 gcgcgtcttt ggtcggcacg tgcgtgtgcg tgcccaagac gagcgacacg cgcccgtcca
     4081 agtagaaccc catcgcgatc ttctcgctgg tcgcttcgcc gtggaagtcg accaggatca
     4141 ccttggcact ctccgggagc tcggacaaga gccggtcggt ggtgcgaaac ggacagtcca
     4201 acggcggcat gaatacgcgt ccctgtacgt tcaatacggc cacgcgcccg tcggtgccgg
     4261 gtaccagcgc cgcgccgcgc ccgggcgcgt cggggtagtt ggcgggccga acgatgttgg
     4321 gtttggaatc gagtagggcg atcccttcgc gtttgtccca cacgtggtta ccggtggtaa
     4381 agaacgcgat gccggccgcc tccatctccg cgatcccggt cgggttgatg ccgaagcctc
     4441 cggccgagtt ttccgcgttg gcgatcaaca ggtcggtgcg gtggcgctcg cgaatacgcg
     4501 gcacgcacca ctcgacggcg cggcgcccgg tcttgccgac cacgtctccc aagaaggcaa
     4561 tgatcatcgt ctcgtcgtcg gtctttctat tttgcgtact cgatcgcgcg cgtctcgcgg
     4621 atcacgacca ctttgatctg gccgggatac tcgatttcct tctccaggcg tcgcgagatg
     4681 tcgaacgcga gctgcttggc ctgctcgtcg tccaccttgc gcccgctcac gacgacgcga
     4741 atctcgcgtc ccgcctggat cgcgtacgac ttctccacac ccgcgaaagc atctgcgatt
     4801 tcttccagtt tggccagtcg cttcacgtaa tgctcgatgg attcgcgccg cgcgcccgga
     4861 cgcgacccac tgaccgcgtc ggccgccgcc acgatgggcg tgtagagcga cgtggtcggg
     4921 acgtcgccgt ggtgggcggc gatcgcgttg accacgtcct cgggctcgcc gtggcggcgc
     4981 gccaattccg cccccaccgt ggtgtgcgaa ccctccgcct cgtgcgacgc cacctttccg
     5041 atgtcgtgca agagccccgc gcggcgcgcg accttgggat ccaaattgag ctcggaggcc
     5101 atgacccccg cgaggtaggc gacctccagc gagtgcgcga ggcagttttg cccgtagctg
     5161 gtgcggaact tcatgcgtcc cacgcagtcg accaaggccg gtgccatccc gttgatgccg
     5221 aggtcgagga gcgcccgttc cccgctctcg cgcacacccg cgtccaccga cgcgcgtact
     5281 tcggcgacga tctcttcgat gcgcccgggg tggatgcgtc cgtcttggac cagcttctcc
     5341 atggccaacc gcgccacctc gcgccgcacg gggtcgaagc tcgagatgag caccgctccc
     5401 ggggtgtcgt cgatgaccac gtcgacgccg gtctccttct cgaaggcgcg gatgtttctg
     5461 ccctcgcgac cgatgatgcg gcccttcatg tcgtcggagg ccaagccgac cacggccacc
     5521 gtggtttcga cgcactgctc ggcggcgtag cgctggatcg cgagcgcgat gatcttgcgg
     5581 ctctcctgct gggcccagcg ctcggcttcc tcgcgaatct ctcgcgcgcg ccgcgatgcg
     5641 tcgacatggg cctcgtcctc gaggtttttc aagagcatgc gcttggcgtc ttcctttcgg
     5701 acaccggcga tgcgctccag gcgcgcgttt tcttcggcga ttagctgcgt cgactccttg
     5761 aggcgctctt cgagcacctt ctgcttggca tcgagggcca gggatcgctc gcccaggccc
     5821 gcgtccttgc ggtccaggat ctcgaggcgc ttggcgagcg cggactcgcg cacgtcggcg
     5881 tcgtggacgc ggcggcgcag gtcttcgcgc gcgccgagca tctcgcgctc gagctcgatg
     5941 cgttgggcgg ccatcgcgtt ctcggcttcc aggctggcgg cgcgaacgat ggagtcggcg
     6001 cgccgcgtgg agtccgcggc catctgttcg gcgagctgct ccgcgcgcag gaggcccgag
     6061 cgcgccaccc tacgcgcgcc ggcgatcccg agcgcgaagc ccgcgatcgc ggcggcgaca
     6121 ccgcccaaga ccacccagag ccagttggga ttcacgggcc ctccgcgccg gcgtccgcgg
     6181 ccggcaatcg cgtggcgtcg gcgtgtccgc cgacgagacg tcgtgcgatc ggtcaggaag
     6241 gcgaggagtg aagagccggc gggaaaagaa aattccccac gctatgccgt gtgcaaggga
     6301 actcatgaac cagtcggtcc acgtggatac ccgtacgaat cgttttgatc tgccgccgaa
     6361 gcggctacag cccgatccga ataaggcgtc cctgaataag gtgttggctc aaaaattacc
     6421 ttacttcatc acgaacatgg tagggaattt tcggttctgc atcggccgcg accaacgcgg
     6481 cccgttgcca tgtccattgc aatcatccgt cgacggacag tttctcgtcg agcaagctga
     6541 tcaagctctc cgcttttcgt tcgatggtcg acatttcttc gtgcgaagcg cggcgcaatt
     6601 ggaacaactc gtccgcgata ttcatcgcgg cgagtatggc cacgcgatcc gaagccggca
     6661 acgatgaccc ctgggagacc tcgcgcacct tttcgtcgac gtagcgcgcg acttcctgga
     6721 cgtactgctc gtcggcggca ccgcgcacgc ggtacgcgcg acccatgatc tcgaccgtcg
     6781 tcgccttcgt gttttccatc tcgacccgtt cgtcgtcggg gttcattttc aacacccagt
     6841 cagtgttcgc ccgcacccgc tacagctgca gctctccgag cttctccagc acgcggtcga
     6901 tacgcttcct gacttcctcc ttgtgaaacg aactcaatat tttatcgtga ttcgatttga
     6961 gatgattgat tctgtcgttc aactcgcggt tcgcgctctt gagtttgtcg atctcgcgct
     7021 cgagcgagct tctctgctcc tgcagcgcgc ggtactggtc cttgaggtgc gcgaagcgtt
     7081 cggcggtctg cgcgatgcga tcttcgagca tcgacagctt ctcgatcgcg ggtttggtct
     7141 ccatcgaaac cgtctccttc tccgctacga ccgcagcacc acgcccaatt cggactcgag
     7201 cttggatacc agcttggcga tgagcgcgtc gacgtcggtg tcggtgagcg tcgcgtcgtt
     7261 cgatcggaac gaaagccgca cgccgtaggc ggtcattccc tcgccgaggc cgccgccgcg
     7321 atagacgtcg aacacctgca gggattctag caaccgtccg ccgaccttga ccacatgttt
     7381 ttcgatctga gcccatacga ttcccgacgg cgcgaccaac gacaagtcgc ggcgggaggg
     7441 cgggtactcg gagtagggct tgaaggccgg tgtggcggcc gcgtgctcga agagcgcgga
     7501 cacgtcgacg accgcgtacc aaaccggctg cgtgaggtcg agctcgcgcg cggcggcgac
     7561 gggcacgatg ccgccttcga ccaccgcgcg gcggcggtcg gagaagcgga aggttccgtg
     7621 ggtgggctcg aactcgtaga cgaggtcggc gtacacgtcg ggcgcgtgcg tcgcgagcag
     7681 ggactcgact tcggccttga tctcgaagaa gtcgaccggc tcggcgcccg cgcgccagaa
     7741 cgcggagccc tcggggcgcg tgcgcgcgat caggatctgc gtgggctcat cgggcagggc
     7801 cgcgctctcc gcggagcgaa cgaacacgcg tcccagcgcg aacaggtaga gctcgcgccg
     7861 gccgcgcgca acattgtgcg cgaccgcgtc gagcgcgccg ggcaagagat gggtgcgcat
     7921 ctgggcgtgg gtcgaagaaa gcgggttctt gagaaccacc gcctggctgc gcggatcgct
     7981 atccgcccag ccgaaccagc gctgctcgcg cccgtcgctg aacgacgtgg tcaggatctc
     8041 cgtgtgcccg cgcgccacca agtgctcggc cagccggtcg acgaatcggt cgaaggggtc
     8101 gggtgcggag ggcaccgtgg agcgatagcg ccaccccttg ccgatgttct cgtagccgta
     8161 cacgcgcgcg acttcttcta ccaaatcggc ttcctgttcg atgtcccagc ggaacgacgg
     8221 caccgcgacc gtgaccgcgt cggcgtccga tcccaccagg gcgaactcga gccggccgag
     8281 gatctccgcg atggcgtcgc tggtgagatt ctctcccagg agccggcgca ccgtcgagcg
     8341 ccgcagcgtg accgcacgcg gttggcgcgg agccgggaac acgtcgaccc acttgcccac
     8401 gcggccgccg gcgacttcca cgatcagcgc cgccgcgcga tcggacgccc gccggcacgc
     8461 ctcgcggtcg ctcccccgct cgaatcgata ggaggcgtcg gtgctgaggc cgagggcccg
     8521 gcgcgtgcgc cggaccgtct tggggtcgaa ggcggccgat tcgagcagga catcggtggt
     8581 cgcctcgttg atgcgcgatg ccattccgcc gatgacgccg gccacaccca ccacgtcgtc
     8641 gcccgcggtg atgaccagat gggagtcgtc gagttcgtag gtggcggcgt cgagcgcctc
     8701 gatgctttcg ccgcgccgcg cgcggcgtac gcccatgcgc gaagacggga gcccgttcac
     8761 gtcgtagccg tgcatcggct gtcccatctc catcatcacg aagttggtga cgtcgacgat
     8821 gttgttgaca ctgtgaaccc cgacccgttc gagcgccccc ttgagccagg cgggcgaggg
     8881 cttgacgacc acaccgtgga tcacgcgcgc ggtgaagcga aaacagtcgg ccgggtcggc
     8941 gatctccacc gcgacctcgg tgtcgtcggg ggtctccacg ggcgtctcga cctgcggcgg
     9001 tcgcaggcgc gtgcgataca gcgcggcgat ctcgcgcgcg acacccacgt gcgaaagctg
     9061 gtcgggccgg ttgggcgtca cctcgagctc gagcaccgcg tcggtttcac cgaggacgtc
     9121 ggcgagtgga acgccgacgg gcgcgtcggg agcgagcacc aggatgccgc cgctctcggt
     9181 gccgagaccc agctcgacct ccgagcagat cattccgttg gaggcgacac cgcggatctt
     9241 gctcttgcgc agcttcactc cgttggggag cttcgccccc accagggcca ccgcgacgcg
     9301 ctggcccgag gccacgttgg gggcaccgca cacgatctcg agcggctcac cctgccccac
     9361 gtcgacacgg cacacgctca agcggtcggc ttccgggtgg cgcttggctt cgagcacgtg
     9421 tcccaccacg accaggcgct cggtgaggcc cgagctcgtg agccgctcga ccttgatgcc
     9481 gtgcatcgtg agatcgtggg cgagcgtggg cgcgtcgacg gtgatgtcga cgtagcgacg
     9541 gagccacgaa agcgagacgt tcatgcgcgc gcgcctctca acgctcggcg aactgatcga
     9601 ggaagcgcag gtcgttggtc aggaacatgc gaatgtcggg gatgccgtgg cggatcatcg
     9661 cgatgcgatc gacgcccacg ccgaacgcga agccgctgta acggtcgggg tcgatgccca
     9721 ccgcgcgcag cacgctcggg tgcaccatgc cggcgcccag gatctcgatc caccccgact
     9781 gcccgcagat cacgcatcct cctccgtgac agttgaaaca cgacatgtcg acgtcgacgc
     9841 tcggctccgt gaacggaaag aagtgcggct tgaagcgcac ctggatgtcg gagccgaaca
     9901 gatcgcgcag gaacaaaagt aggtcgccgc gcaaatcggc catggtgacg tcggtgtcga
     9961 tgtagaggcc ctcgacctgg tggaattcgg cttggtgcga cgcgtccagg gcttcgttgc
    10021 ggtacacgcg cccgggtgcg tacaccttca gcggcggctt gtgctcttgc atgtaacgca
    10081 cctggatggg cgaggtgtgt gtgcgcaaga ggatgtcgtt cgagacgtag aaggtgtcct
    10141 gcaggtcgcg cgacgggtgc tcgtcgggaa aattgagcgc ctggaaattg tagtactcga
    10201 gctcgacctc ggggccgcgt gcgagccggt accccatgcc ctcgagcacc gagccgatgc
    10261 gccggaaggt ttgggagatg acgtgacggc ggccgcagag cccgttggtg gccggaaggg
    10321 ttccgtcgac gggggcggcg tagtccgcgc gctcgtcggc gccgcccagt tccgcgcgcc
    10381 gctccgcgag cagggtctcg cccagcgcct tgaggcggtt ggcttcggca cccaggcgcg
    10441 ggcgatcttc cgcgcgcgcc tcgccgatcg cgcgcaggaa cgcggtgagc acgcccttgc
    10501 ggcccaggta cttgacccgc gccgcttcga gctcatcggg ggtcgagcac gcggcgacgt
    10561 cggcgc
//
LOCUS       DASPGK010000116         5468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1669928, whole genome shotgun sequence.
ACCESSION   DASPGK010000116 DASPGK010000000
VERSION     DASPGK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669928"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEC56_03345"
     CDS             <1..366
                     /locus_tag="VEC56_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014927672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV13219.1"
                     /translation="REGQSMIHGLHAMFYSSKAVELREFFRDKLGFDGTDVGDGWLIF
                     DLPEADLGVHPTDREGPASGTADISFYCDDLEATVETLRARGVEFTREVEDHGYGLVT
                     FFKAPGDFVIQLYQPKYRK"
     gene            363..923
                     /locus_tag="VEC56_03350"
     CDS             363..923
                     /locus_tag="VEC56_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016914875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXV13220.1"
                     /translation="MSARRLVRDDFNRPTLKVARGLVGTYIVRSLNGRRIAAMITETE
                     AYKGPRDAASHAFAGRRTARVEPLYRDGGTAYVYLVYGMHWLLNFSTAGAGKPEGVLI
                     RAVVLENDEPVIGPGRVTRALHIDKSLDGVDVVSSKELWLEDRGVRVPARRIQTGPRI
                     GVDYAGPYWAARPWRFWTDGPALASR"
     gene            920..1504
                     /locus_tag="VEC56_03355"
     CDS             920..1504
                     /locus_tag="VEC56_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HXV13221.1"
                     /translation="MKVRHFKSATEFRTWLAKNHAAERELWVGYYKKETGKGGITWQE
                     SVDEALCYGWIDGIRKKVDAESYTNRFSPRTTKSTWSSINIRRAQALIDAGRMRAAGL
                     AAFESRREYRSGIYSYEQRKDQLDQPYQGRLKRNQKAWAFYQGQTPGYRRLANWWVVS
                     AKREATRLKRLDQLIELSAQGKTIRELAKKPKAK"
     gene            1510..2412
                     /locus_tag="VEC56_03360"
     CDS             1510..2412
                     /locus_tag="VEC56_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXV13222.1"
                     /translation="MSIRAVKQITQSQLTMEGAGVKLRRAFGFGNTDEFDPFLLFDDF
                     RNENPNDYLAGFPWHPHRGIETITYVLAGEVSHGDSLGNRGTLGAGDVQWMTAGSGIL
                     HQEMPQGDAKGRMHGFQLWANLPRSLKMTQPRYQDVSGRDIPEIVDDDGTRVRIVVGD
                     FWGKRGPVEGIAADPRYLDVSVPAGRRKTLPVETTRHAFAYVFEGSGTFRDASQPQAV
                     ATDRIGPSGEEIRDPVGNRSLVLFDNGDEVTVQAGPDGIRFLLVSGKPLEEPVAWQGP
                     IVMNTPEELRQAYAELRNGTFIKR"
     gene            2517..2960
                     /locus_tag="VEC56_03365"
     CDS             2517..2960
                     /locus_tag="VEC56_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13223.1"
                     /translation="MEFLRVAKRIGIRALAALMVIGGALAIALIRALKALANPTDLLA
                     RLRRRALLWPWLTQSYKQVIGMVAYNAFVFFLVEPILRRWFPPLKPWEHVTGIFSGGT
                     RTTYEEIVPTVATALWIAGNALVIVFLRDAERKGSHAGTGAHTTL"
     gene            3062..3892
                     /gene="otsB"
                     /locus_tag="VEC56_03370"
     CDS             3062..3892
                     /gene="otsB"
                     /locus_tag="VEC56_03370"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HXV13224.1"
                     /translation="MQALRPTFDIDRFFSRVRSSSTRVLMLDYDGTLAPFRDRPAEAV
                     PYPGVIPLLDTMMEAGHTRVVIVSGRWARDLLPLIRLKRRPELWGSHGWERVRTSEEY
                     ESARVGPGVAEMLVAVDEWTEEIEALGGRVENKPAGVAFHWRGLSGDRVAAIRYALTV
                     RWAALASSNEIEWHDFDGGIEIRAAGRNKGDVVRALFAEAGSSAAIAYLGDDLTDEDA
                     FEAVPPHGVAVLVREQLRSTAADVWIKPPDELLEFLRRWHETARGQDHKESRDADGTC
                     "
     gene            3873..>5468
                     /locus_tag="VEC56_03375"
     CDS             3873..>5468
                     /locus_tag="VEC56_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXV13225.1"
                     /translation="MPTERADSGLELGLIGNCNVSALIDARGRFVWSCLPRYDGDPVF
                     CVLLNEGADAGGAFAIELLNLARSEQRYLRNTAILVTELHDEDDGVVEITDFAPRFRQ
                     HGRMFYPAMFIRRVRRLSGSPVVRVRLQPRYGYGKSTPSLTFGSNHIRYVGPEVVMRL
                     TTDASVTAVLEETAFAVDRDLTFVLGPDETVTESVAYVGARFLGETRSYWLDWVRFLG
                     IPFEWQEAVIRAAITLKLSAYDDTGAIVAAMTTSIPEAPDSGRNWDYRYCWLRDSYFV
                     VAALNRLSATSTMERYLGYIVNIAATAPGGSLQPVYGVSGRAALDEMVVDGLPGFRGM
                     GPVRVGNAAYAQTQNDVYGSAVLSATHVFFDERLERSGDPALFQRLEALGEAAVNVHQ
                     RPDVGIWEFRSNSKVHTFSAVMCWVACDRLARIALRLDLRDRANVWRERADAMRVVIE
                     KRAWNAKLGSFVDSFEGKDLDGSLLLLHEVGFLAAADPRFAGTVAAVERHLRKGDFVY
                     RYLRPDDFGVPQSAFVVCTFWYID"
BASE COUNT          946 a         1738 c         1837 g          947 t
ORIGIN      
        1 agggagggcc aatccatgat ccatggtctg cacgcgatgt tctattcctc gaaggcggtc
       61 gaactgcgcg agttctttcg cgacaagctc ggcttcgacg gtaccgacgt cggcgacggc
      121 tggctcatct tcgaccttcc cgaagccgac ctcggcgttc accccaccga ccgcgaaggg
      181 ccggcttctg gaaccgcgga catctcgttc tattgcgacg acctggaggc cacggtcgaa
      241 accctgcggg cgcgcggggt cgagttcacg cgcgaggtcg aagatcacgg ctacggcctg
      301 gtcaccttct tcaaggctcc gggcgacttc gtcatccagc tctaccaacc caagtatcgc
      361 aaatgagcgc gcggcgcttg gtgcgcgacg acttcaaccg gccgaccctg aaggtggcgc
      421 gggggttggt cggtacttat attgtgcgaa gcctgaacgg ccgccgcatc gcggccatga
      481 tcacggagac cgaggcatac aaggggcccc gggacgccgc gtcgcacgcg ttcgcggggc
      541 ggcgcacggc ccgcgtcgag ccgctgtatc gcgacggtgg gaccgcctac gtctacctcg
      601 tctacggaat gcattggctc ttgaatttca gcaccgcggg tgcgggcaag cccgaaggcg
      661 tgttgattcg cgccgtcgtg ctcgagaacg acgagccggt gatcggaccc ggtcgcgtga
      721 cgcgcgcgtt gcacatcgat aagagtttgg acggtgtcga cgtggtttcg tcgaaggagt
      781 tgtggctgga agatcgcggc gtgcgtgttc ccgcgcgccg gatccagacc gggccgcgca
      841 tcggcgtcga ctacgcgggc ccctactggg ccgcgcgccc gtggcgcttc tggaccgacg
      901 gcccggcact cgcatcgcga tgaaggttcg gcacttcaaa tccgccaccg agtttcgcac
      961 ctggctcgcg aagaaccacg cggccgaacg cgaactatgg gtgggctact acaagaagga
     1021 gaccggcaaa ggcggcatca cctggcagga gtccgtcgac gaggcgctct gctacggttg
     1081 gatcgacggc atccgcaaaa aggtggatgc ggagagctac accaaccggt tctcgccgcg
     1141 cacgacgaag agcacctgga gctcgatcaa catccgacgc gcgcaggcgc tgatcgacgc
     1201 gggacgcatg cgcgcggcgg gcctggccgc cttcgaatcg cgccgggagt atcggtccgg
     1261 catctactcc tacgagcagc gtaaggatca actcgaccaa ccgtaccaag ggcggctcaa
     1321 gcgcaaccag aaggcgtggg cattctatca gggacagacc cccggatacc gtcgcctggc
     1381 gaactggtgg gtcgtgagtg cgaagcggga ggccacgcgc ctcaaacgcc tcgaccagct
     1441 gatcgagctc tcggcgcagg gaaagacgat tcgagagctg gctaaaaaac cgaaagcgaa
     1501 gtgatttcga tgtcgatacg agccgtaaag cagatcacgc aatcgcaatt gaccatggag
     1561 ggcgcgggag tgaagctccg tcgcgcgttc ggcttcggca acacggacga gttcgacccg
     1621 tttctgttgt tcgatgattt ccgcaacgag aacccgaacg actacctggc cggctttccg
     1681 tggcatccgc accgcggcat cgagacgatc acgtacgtcc tcgcgggcga agtgtcccac
     1741 ggcgacagct tgggtaatcg cggcaccctc ggtgcgggcg acgtgcaatg gatgacggcg
     1801 ggaagcggca ttctccacca agagatgccg cagggagacg ccaagggacg catgcacggc
     1861 ttccagttgt gggcgaatct cccgcgctca ttgaagatga cgcagccgcg ctatcaagac
     1921 gtgtcgggcc gcgacatccc cgagatcgtg gacgacgacg gcacgcgcgt gcgcatcgtg
     1981 gtaggcgatt tctggggcaa gcgcggaccc gtcgaaggaa tcgccgccga cccgcgctac
     2041 ctcgacgtgt ccgtgccggc gggccggcgc aagacgctcc cggtcgagac cacgcgccac
     2101 gccttcgcgt acgtgttcga aggctccggg accttccgcg atgcgtcgca gccccaagcg
     2161 gtcgcgaccg accgcatcgg tccctcgggc gaggagatcc gcgacccggt gggcaaccgt
     2221 tcgctggtct tgttcgacaa cggcgacgaa gtgaccgtgc aagcgggacc ggacgggatt
     2281 cgtttcctgt tggtttccgg aaaaccactc gaagaaccgg tcgcgtggca gggccccatc
     2341 gtgatgaaca cgccggaaga gcttcgccag gcctacgccg agcttcgaaa cgggacgttc
     2401 atcaagcgat agaggcaggc gcggatattg ctcattcctc ggcggtcccc cggtgcatcg
     2461 aggtcgcgac gggcgccaag cgccatcgtc ggaaatcacg tggagcaagg accgtcgtgg
     2521 agtttcttcg cgttgcaaag cggatcggta ttcgcgcgct ggcggccctc atggtgatcg
     2581 ggggtgcgct cgcgatcgca ttgatccggg ccctcaaggc actcgcgaat cccaccgacc
     2641 tactcgcccg gcttcgccgg cgcgcgttgc tctggccgtg gctcacgcag tcgtacaagc
     2701 aggtcatcgg aatggtggcc tacaatgcct tcgtcttctt tctcgtggag cccatcctgc
     2761 gccgctggtt ccctccgctc aagccctggg agcacgtgac gggcatcttc tcgggaggca
     2821 cgcgcaccac ctacgaagaa atcgtcccga cggtcgcgac ggcgctctgg atcgcgggca
     2881 acgctctggt catcgtgttc ctgcgcgacg ccgagcgaaa gggctcccac gcgggcaccg
     2941 gtgcgcacac caccctgtga gggtccgcga agttcctcgc ccattgcact tgcccatcgc
     3001 ccgccttgtg gcctagcctg cttgcgaggg gtctgcttat cgacgcacct ggtggcgtac
     3061 catgcaggca ttgcgtccga cgttcgacat cgaccgtttc ttctcccgtg tgagatcgag
     3121 cagcacgcgc gtgctgatgc tcgactacga tgggacgctg gcgccgtttc gcgaccggcc
     3181 ggcggaagcc gtgccttacc cgggcgtcat tccgctgctc gacacgatga tggaagccgg
     3241 gcacacgcgt gttgtcatcg tgagcgggcg ctgggcccgc gacctcctgc ccctgattcg
     3301 actcaaacga cgccccgaac tatggggctc gcacggctgg gagcgtgtgc gcaccagcga
     3361 ggagtacgag agcgcgcggg tgggtccggg cgtggccgag atgctcgtgg ccgtagatga
     3421 atggacggaa gagatcgagg cgctgggtgg tcgcgtcgag aacaaaccgg ccggcgtcgc
     3481 gttccattgg cgcggacttt ccggcgaccg ggtcgcggcg attcgttacg cgctgaccgt
     3541 gcgttgggcg gcgctcgcaa gctcgaacga aatcgagtgg cacgacttcg acggcggcat
     3601 cgagatccgc gcggcggggc gcaacaaggg cgacgtggtg cgcgcactct tcgcggaggc
     3661 gggaagctcg gccgcgatcg cttacctagg ggacgacctc accgacgagg acgcgttcga
     3721 ggccgttccg ccgcacggcg tcgccgttct ggttcgagag caactgcggt ccaccgcggc
     3781 cgacgtttgg atcaaaccgc ccgacgagct gctggagttc ctgcgtcgct ggcacgagac
     3841 ggcccgcggg caggatcaca aggagagtcg agatgccgac ggaacgtgct gacagcggtc
     3901 tggaacttgg gctgatcggc aactgcaacg tgagcgcgct catcgacgca cgcggccgat
     3961 tcgtatggtc ctgcctgccg cggtacgacg gcgatcccgt gttctgcgtg ctgttgaacg
     4021 aaggtgccga cgccggcggc gcgttcgcga tcgagctgct gaacctggct cggagcgagc
     4081 agcgctatct ccgaaacacc gcgatcttgg tgacggagct gcacgacgaa gacgacggcg
     4141 tggtcgagat cacggatttc gccccgcgct tccggcagca cggacgcatg ttctaccccg
     4201 cgatgttcat ccggcgcgtg aggaggttgt cgggtagccc cgtcgtgcgc gtccgactgc
     4261 agccgcgcta cggttacggc aagagcacgc cctcgctgac cttcggcagc aatcatattc
     4321 gttacgtcgg gccggaggtc gtcatgcgac tgaccaccga tgcgtcggtg accgcggtgc
     4381 tcgaagaaac tgccttcgcg gtcgatcgcg acctgacctt cgtgctgggg cccgacgaga
     4441 cggttaccga gtcggtggcc tacgtcggcg cccgtttcct gggcgagacc cggagctatt
     4501 ggctcgattg ggttcgattc ctgggaattc cattcgagtg gcaggaggcg gtgattcgcg
     4561 ccgcgatcac actcaagctc agcgcctacg acgacacggg tgcgatcgtg gcggcgatga
     4621 cgacctcgat tcccgaggcc cccgacagcg gccggaattg ggactaccgc tactgctggc
     4681 ttcgcgacag ctacttcgtg gtcgccgcgc tcaatcgctt gagtgccacc agcacgatgg
     4741 aacggtacct cgggtacatt gtcaatatcg ccgcgaccgc gccgggcgga agcttgcagc
     4801 ccgtgtacgg agtgagcggc cgcgcggcgt tggacgaaat ggtcgtcgac ggtttgccgg
     4861 gtttccgagg catggggccg gtgcgcgtgg gcaacgccgc gtacgctcaa acgcagaacg
     4921 acgtctatgg ctccgcggtg ctatccgcca cgcacgtctt cttcgacgaa cgcctcgagc
     4981 gttccggcga cccggcgttg ttccaacggc tggaggcgct cggcgaagcc gcggtgaacg
     5041 tccaccaacg gcccgacgtc gggatttggg agtttcgatc gaactcgaag gtacacacgt
     5101 tctccgccgt catgtgctgg gtcgcctgcg accggctcgc ccgcatcgcg ttgcgcctcg
     5161 atctgcgcga ccgcgcgaac gtctggcgcg agcgggccga cgccatgcgc gtcgtgatcg
     5221 agaaacgggc gtggaacgcg aagctgggca gcttcgtgga ttcgttcgaa ggcaaagacc
     5281 tcgacggcag tctcctgctg ctgcacgagg tgggtttcct cgccgccgcc gaccccaggt
     5341 tcgcggggac tgtggccgcg gtggagcggc acttgcgcaa gggcgatttc gtgtaccggt
     5401 accttcgccc cgatgacttc ggcgttcctc aaagcgcgtt cgtggtgtgc accttctggt
     5461 acatcgac
//
LOCUS       DASPGK010000117         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_733425, whole genome shotgun sequence.
ACCESSION   DASPGK010000117 DASPGK010000000
VERSION     DASPGK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733425"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1079
                     /gene="purM"
                     /locus_tag="VEC56_03380"
     CDS             69..1079
                     /gene="purM"
                     /locus_tag="VEC56_03380"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXV13226.1"
                     /translation="MRYRDSGVDIDKANEATRAIARLAKATWGPEVLSEIGHFGGLFA
                     VPKNYRDPVLVSSMDGVGTKLLVARAAQKFDTVGQDLVNHCVNDILVQGAHPLFFLDY
                     VAAGKLEPAMVASVVAGLAQACKENHCALIGGETAEMPGLYQHGDFDLAGTIVGVVER
                     DGVIDGSAIVAGDLVFALPSNGLHTNGYSLARRVFEQAGLALDARVEDFGSTVAEELL
                     RVHRSYLAPVKEIAERVRIKGLAHITGGGILENLPRILPDRTGARIHKGTWPVLPIFD
                     YIARAGDVVEGEMYRVFNMGAGMLIVVSAQDAAKVPERAAGLTVYRVGEIVKGDGSVA
                     LV"
     gene            complement(1076..2461)
                     /locus_tag="VEC56_03385"
     CDS             complement(1076..2461)
                     /locus_tag="VEC56_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV13227.1"
                     /translation="MSKSRLRSGVGRSSGLVALGLVVFCAACEDDTGPGPAPADIEIV
                     SGNGQYSKQGTELEDPVVVQVTLTDGEVGSGVTVSFSVRSGGGSLSRASATTNSDGQT
                     SVRWTLGPTLGAQQISISVTGDAEVATVAQATAGIYSCPEEDPTFVQRFSPSHDLFLF
                     SHASSATVSGGTPRAGVVHLGLDLMNTEFDASSFVAFDWTVLQAVVRDCAFSQSGEFY
                     IAWTNTSADREIAKVAPDRSVTHFATLEGLLGTEITWIEDGVLGGVDEFGPFTVGCRD
                     TLTRYADATFIGTEPNNANFDAVAYDAAGDYLYFIDRENRRLKRIPLDGYTQTGATEE
                     VVTLEPDESTGAVGMAVHTDGAVYLVVESAGTKAIVKVVPGVSKEIAFDFFSRGGGNA
                     AGIQDDLAIDSAPTRGYLYTLDTLNNVILLYRISDGQLFELDPDGNTDPNAASSTSSG
                     ERVGLAVLPGT"
     gene            2633..3181
                     /locus_tag="VEC56_03390"
     CDS             2633..3181
                     /locus_tag="VEC56_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HXV13228.1"
                     /translation="MAKRVIVPIANGFEEIEAVVIVDILRRAGLDVVVAGVEGADAIG
                     SHDIALRCDVMLDECDRADAIVLPGGMPGSKRLRESDAVRSWVTRLNAEGKLVAAICA
                     APTVLESCGVLEGRRATSHPSHADEMKRCVYQTADVVTDGNVVTSRGAGTAIAFAAEV
                     VRHLVDDATANEILARIQYTTQ"
     gene            complement(3185..>3437)
                     /locus_tag="VEC56_03395"
     CDS             complement(3185..>3437)
                     /locus_tag="VEC56_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13229.1"
                     /translation="LTLADVNGAIKRHLRADRARIGVVTEGADAFLRDLITNQPSPMK
                     YDAEGIAQDVLTEDAAIAVYQLNINREKSKIVDVDEMFE"
BASE COUNT          580 a         1076 c         1206 g          575 t
ORIGIN      
        1 ctcatcgtcg agaacgatcc gaccaccgcc atctacgaat cgctgcaaga aaggctatag
       61 aggatcccat gcgttaccga gattcgggcg tcgacatcga caaggccaac gaggcgacgc
      121 gcgcaatcgc gcgcctggcg aaggcgacgt ggggacccga ggtactctcg gagatcggac
      181 acttcggggg actgttcgcg gtgcccaaga actaccgcga cccggtactg gtgagcagca
      241 tggacggggt cggcaccaag ctcctggtgg cgcgcgcggc gcagaagttc gacaccgttg
      301 ggcaggacct cgtcaatcac tgcgtcaacg acatcctcgt gcagggagcg cacccgctct
      361 tcttcctgga ctacgtcgcg gccggcaagc tggaacccgc gatggtggcg tccgtggtgg
      421 cggggctggc gcaggcgtgc aaggagaacc attgcgcgct gatcggcggt gagacggccg
      481 agatgccggg actgtatcag cacggcgact tcgacctggc ggggaccatc gtgggcgtcg
      541 tcgagcgcga tggggtgatc gacggctccg cgatcgtcgc cggcgatttg gtgttcgcgc
      601 ttccatcgaa cgggctgcac accaacggct actccctggc acggcgcgtg ttcgaacaag
      661 ccggtcttgc gctggacgca cgcgtggagg atttcggctc cacggtagcg gaagagttgc
      721 tgcgcgtgca ccgctcgtat ctggctccgg tcaaggagat cgcggagagg gtccgcatca
      781 agggactcgc ccacatcacc ggcggcggga tactggagaa cctcccgcgt atcctgcccg
      841 accgcaccgg tgcccgcatc cacaagggca cctggccggt gctgccgatc ttcgactaca
      901 tcgcgcgcgc cggcgacgtg gtggaagggg agatgtaccg cgtgttcaac atgggcgcgg
      961 ggatgctgat cgtggtctcc gcccaggacg cggccaaggt ccccgagcgc gccgcgggac
     1021 ttacggtcta ccgggtcggg gagatcgtca agggcgacgg atcggtggcg ctggtctagg
     1081 tcccgggcaa gaccgccaag ccgacgcgct ctcccgagga tgtgctcgac gcggcgttgg
     1141 ggtcggtgtt tccgtccggg tccagctcga agagttgtcc atcgctgatc cggtagagca
     1201 agataacatt gttcagcgtg tccagcgtgt agaggtatcc gcgcgtcggc gccgaatcga
     1261 tggccagatc gtcctggatg ccggctgcgt tgcccccccc gcgcgagaag aaatcgaaag
     1321 cgatctcctt cgacaccccc ggcacgacct tcacgatcgc tttcgttccg gccgattcca
     1381 ccacgaggta gaccgcgccg tcggtgtgca cggccatgcc gaccgcaccc gtggactcgt
     1441 cgggttccaa tgtgacgacc tcttcggtcg caccggtctg cgtgtagccg tcgagcggaa
     1501 ttcgcttcaa gcgccggttc tcacggtcga tgaagtagag gtagtcgccg gcggcgtcgt
     1561 acgccaccgc gtcgaagttg gcgttgttgg gctcggtgcc gatgaaggtg gcgtccgcgt
     1621 agcgggtgag ggtgtcgcgg cagcccacgg tgaagggccc gaactcgtcg acgccgccca
     1681 ggactccgtc ttcgatccac gtgatctcgg tgccgaggag tccctcgagg gtcgcgaagt
     1741 gggtgacgga acggtccggg gcgaccttgg cgatctcgcg gtcggccgac gtgttggtcc
     1801 acgcgatgta gaattcgccg ctttgcgaga aagcgcaatc gcggaccacg gcctgcagga
     1861 cggtccaatc gaaagccacg aagctgctgg cgtcgaactc cgtgttcatc aggtccaatc
     1921 cgagatgaac gacccccgcg cgcggggtgc cgccggagac cgtagcgctc gaggcgtgcg
     1981 aaaagaggaa caggtcgtgc gacggcgaga accgctgcac gaaggtcggg tcttcttcgg
     2041 gacagctgta gataccggcc gtcgcctgcg cgacggtggc aacctcggcg tcgccggtga
     2101 ccgagatcga gatctgctgg gcgccgaggg tcggcccgag ggtccagcgc acgctggtct
     2161 gtccatcgct gttggtggtg gcactggcgc gcgagaggct gccgccgccg ctccggacgc
     2221 tgaagctcac cgtcacgccg gagcctactt cgccgtccgt gagcgtcacc tgaaccacca
     2281 ccgggtcctc gagctcggtc ccctgcttgg aatactgccc gttgcctgat acgatttcga
     2341 tgtcggccgg agcggggccg gggccggtgt cgtcttcgca cgcggcgcag aagacgacca
     2401 gcccgagcgc gacgaggccg gatgaacgac cgacaccaga gcgtaaccgt gacttcgaca
     2461 tgagcagcgt cctgtggttt gaaactgttc gatggggacg gcgcccgggc gacgcgggcc
     2521 gtggggcgac cccggggtgt tcgcgccggg aatgcagtgg gacgataaca ccccagccgg
     2581 gcttcgtcaa tcgaacgagt gcggatcgaa tgcgcgagtg gggaggttct gcatggccaa
     2641 acgagtgatc gtgccgatcg cgaacgggtt cgaagagatc gaagcggtgg ttatcgtcga
     2701 catcctgcga cgagccggcc tggacgtggt cgtcgcgggg gtcgagggcg cggatgcgat
     2761 cggctcacac gacatcgcgc tccgctgcga cgtgatgctc gacgaatgcg atcgcgccga
     2821 cgcgatcgtc ttgccgggcg gcatgccggg gagcaagcgg ctgcgcgaga gcgacgcggt
     2881 tcggtcgtgg gtgacgcggc tcaacgccga aggcaagctg gtggccgcga tctgcgccgc
     2941 tcccaccgtc ctggagtcgt gcggtgttct ggaaggccgt cgcgcgacca gtcaccccag
     3001 tcacgccgac gagatgaagc gctgcgtcta ccagaccgcg gacgtggtga ccgacggcaa
     3061 cgtggtcacg agccgcggcg ccggcaccgc catcgcgttc gccgccgaag tggttcggca
     3121 cctggtcgac gacgccaccg cgaacgagat cctggcgcgc attcagtaca cgacccagta
     3181 gcggctactc gaacatctcg tcgacgtcga cgatcttcga cttctcgcgg ttgatgttga
     3241 gctggtagac cgcgatcgcc gcgtcctcgg tgaggacgtc ctgcgcaatt ccctccgcgt
     3301 catatttcat gggagaaggc tgattggtga tcaggtcgcg caggaaggcg tccgcgccct
     3361 cggtcacgac cccgatgcgc gcgcggtcgg cgcgcaggtg gcgcttgatg gccccgttga
     3421 cgtcggccag cgtcaac
//
LOCUS       DASPGK010000118         6250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1202583, whole genome shotgun sequence.
ACCESSION   DASPGK010000118 DASPGK010000000
VERSION     DASPGK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202583"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VEC56_03400"
     CDS             complement(<1..1434)
                     /locus_tag="VEC56_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXV13230.1"
                     /translation="MRPRIQIYTAVLTLWAVLATGCASTGDSSSARGRSVAQRNLEPR
                     VDYGRSIADFSSRVAASRSLYANNDVAGFLSARDSLLADVNDFIRDHPHAEQDPDFVR
                     LLNSLSALDTLVVNQPRVPLYSAVDDSLALAARPWPDADITASDEARLFAMSDSVFPF
                     IESPRIDFWISYFTGPGRERFERAMYRMETHRPTVEAILAEKGMPHELICIAFIESGF
                     AMKAVSSARAVGPWQFINGTARRYNLRVNWWFDERCDIVASTYAAANYLTDLYGIWGD
                     WFLAFAAYNCGEYRVARQVARQKTTNFWQLDLPLQTERYVPKFLAALYIMRDPEKYGF
                     TIPTVEPNRFDLVTVSVPTDLDVIARGAGTTTEVIRDMNPQLKRGATPPMMEVHVKVP
                     RGAGEACTAALANVPPSERVAWTEHKVKKGETLSHIAGQYGTSVDALRDTNNLKRSSM
                     LRVGQIVVVPVPGGSYAEAASSKPTYMN"
     gene            1642..3024
                     /locus_tag="VEC56_03405"
     CDS             1642..3024
                     /locus_tag="VEC56_03405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXV13231.1"
                     /translation="MRIYDRDFRYLLVNLVPQHRLSPDLRRDVDRALRSGDAAVLRRE
                     SVRALEELCESTYFERTGVRADDGTVVVEYRKRGGRFQLSVALPRSEWEDLGDEPRAP
                     EIVLSGDEEVARAPVAWVSPRRPSAEPVTPPVPPSAVLLPDIIRSFTIADRSTPVWER
                     LETLLESLERWLGFAAVRLDVIEDTLIGGGGPGRRVHVVAENELRANDAIARVFESGA
                     RRVVERRDADDEAPGAPESWGSLGVAPIFALGSVVGALSVYYPAGSPRDAMDANLDLA
                     AGVVRQAIEFNHHFESLTSIDALTGIYNRQFFDRQMPVEIERAMRSGSALSMLVLDLD
                     DFKRINDELGHKKGDEALVAVAEIIRKNLRKVDLAFRYGGEEVVILLPGTPEFEAVHT
                     AERLRRVIQQHRGFRDARGAPREITVSVGVAVYPDTAKSADELFVQADEAMYRAKQRG
                     KNQVVLYSGG"
     gene            3052..4527
                     /locus_tag="VEC56_03410"
     CDS             3052..4527
                     /locus_tag="VEC56_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXV13232.1"
                     /translation="MVWLFSLGVVAGVYRSFSLIVILMLALLARAALLRAAHAWVCVP
                     LALACGVVGWASIERVDADRGAVERAARASGGAAIRLCGWVDEFPQSGRYGTTFSLAT
                     RVEGRPVRLWVRAGRFDVGYGDSLAIDARLASASRTPSVFLVSRGLAGEARARFEDVR
                     EIGTPRGCPLLREVLWPMHRAARCNLARALGGEAALPVGLLLGERGMLDRRAHDAVRG
                     LGITHLLALSGMHLTMIAALAVFATRWSPRRRDTVVALALSLYVGVVGNVDSLTRAWW
                     MALLILASRALVRPPRPLDALGKALFLMLLCAPHAVLSVGLQLSFVATFAVLVSLERL
                     PSALLRSPPARAPLWRRAGIRAGQGAAIAFMVSLVVEVFIAPLQVHHFGRISVVGPLA
                     TVVFLIPVTVLQGMALAASFGLPVVGEPLAALLAWASRATCEAIVLAGTTAPEPLEAP
                     EPHWFWYYAAVLVVCARPRRAVAWAMAAAGIALSLRLGAGS"
     gene            4616..5260
                     /gene="purN"
                     /locus_tag="VEC56_03415"
     CDS             4616..5260
                     /gene="purN"
                     /locus_tag="VEC56_03415"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXV13233.1"
                     /translation="MTVTTGRCRVAVLASGRGSNFRALAERCQDKTFPAEVACLMTDN
                     RSAGAIEIADAFGIPWHAVDAGEKRGRLKPGAESEIVAHCQSARTDLVLLAGFMRILE
                     DAPGGLLDCFRSRVINVHPSLLPSFKGLHAQRQALEHGVKVAGCTVHFVDASVDGGPI
                     VLQSAVPVRDDDDVESLSARILEEEHRAVTRAVELFARGQLKVEGRRVLGTDEP"
     gene            5257..6156
                     /locus_tag="VEC56_03420"
     CDS             5257..6156
                     /locus_tag="VEC56_03420"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXV13234.1"
                     /translation="MIKAIGSTTELGLKPDRAGKVRDVFDIGDRLLIVSTDRLSAYDV
                     VLPTRLPGKGVLLTQITLGWYEHFGRSLATHFLTAEVSEYPAPFRGVDALAGRSMLVA
                     KAKRFDVECVVRGYLSGSGWKEYQRNRSVCGIALPDGLSESAELPEPIFTPATKADSG
                     HDENISFEQMCEIVPRRDAEALRAQSIDLYRRGRDYARTRGILLADTKFEFGVIDGQI
                     KLIDEVLTPDSSRFWPADQYAPGRAQESFDKQFVRDYLDASGWDHNPPGPELPGDVVT
                     RTIARYKEAHDLLFPGRNVARYL"
BASE COUNT          933 a         2014 c         2235 g         1068 t
ORIGIN      
        1 attcatgtac gtgggcttcg agctcgccgc ctcggcgtag ctgccgccgg gcacggggac
       61 cacgacgatc tgccccacgc gcagcatgct cgaccgcttg agattgttgg tgtcgcgcag
      121 ggcgtcgacc gacgtgccgt actggccggc gatgtgcgag agcgtctcgc ccttcttgac
      181 cttgtgctcg gtccacgcga cgcgctcgga cggcggcacg ttggcgaggg ccgccgtgca
      241 cgcctcgccc gccccccgcg gtaccttgac gtgcacctcc atcatgggag gcgtggcgcc
      301 gcgcttgagc tgcggattca tgtcgcggat cacttcggtg gtggtgccgg ccccacgcgc
      361 gatcacgtcg aggtcggtgg gcacgctcac cgtgaccagg tcgaagcggt tgggctcgac
      421 ggtcggaatg gtgaatccgt acttctcggg gtcgcgcatg atgtagagcg cggccaggaa
      481 cttcggaacg tagcgttcgg tctgtagcgg gagatcgagc tgccagaagt tcgtggtctt
      541 ctggcgcgcg acctggcgcg ccacgcggta ctcgccgcag ttgtacgcgg cgaaggccag
      601 gaaccagtcg ccccagatgc catacaagtc cgtgagatag ttggcggccg cgtacgtcga
      661 cgcgacgatg tcgcagcgct cgtcgaacca ccagttcacg cgcaggttgt agcggcgcgc
      721 ggtcccgttg atgaactgcc acggccccac cgcgcgcgcg ctcgagaccg ccttcatggc
      781 aaagccgctc tcgatgaacg cgatgcagat gagctcgtgg ggcatgccct tctcggccag
      841 gatggcctcg accgtgggcc ggtgggtttc catgcgatac atcgcgcgct caaagcgctc
      901 gcgtccgggc cccgtgaagt agctgatcca gaagtcgatg cgaggactct cgatgaaggg
      961 gaacaccgaa tcgctcatcg caaacaggcg cgcctcgtcg gaggcggtga tgtcggcgtc
     1021 cggccacggg cgcgcggcca gcgcgaggct gtcgtcgacc gccgaataca agggaacgcg
     1081 cggctggttg acgaccagcg tgtcgagcgc ggagagcgaa ttgagcaggc ggacgaagtc
     1141 ggggtcttgc tccgcgtgcg gatgatcgcg gatgaaatcg ttcacgtcgg cgagcaacga
     1201 gtcgcgcgcg gacaggaagc cggcgacgtc gttgtttgca tacaggctgc gcgaggcggc
     1261 aacgcgcgag ctgaagtccg cgatagaacg cccgtaatcg acgcggggtt cgaggttgcg
     1321 ttgagcgacg gagcgcccgc gggcactgct gctatcgccg gtggaagcgc agccggtcgc
     1381 gaggacggcc cacagggtca ggacggcggt gtagatctga attctcggac gcaccggagc
     1441 ctcctcgagg tcgaatgctg tgtcggatga acgacttggg gcaatatcgg gtcttccagg
     1501 agcaacttgc aagccaaaag ttcccacccc cccgaagagg gggaagttgg cgtacgaaag
     1561 ccctggaagg gtcctatcgg attgcgttaa gatcaatgcg gcagaaaagt aaccagcgac
     1621 gggacagaag gtaccgacgc gatgcgcatc tacgataggg acttccgata ccttctcgtc
     1681 aacctcgttc cacaacaccg actttcgccg gacctccggc gcgacgtcga tcgggccctc
     1741 cgcagcgggg atgccgcggt cctgcgtcgc gagtcggtgc gggcgctcga ggaactgtgc
     1801 gagtcgacct acttcgagcg gaccggggtt cgggccgacg acggcaccgt cgtggtcgaa
     1861 taccgcaagc gcgggggccg tttccagctc agtgtggcgc ttccgcgcag cgagtgggag
     1921 gacttgggtg acgagccccg cgcgcccgag atcgtccttt cgggcgacga agaggtcgcc
     1981 cgcgctcccg tcgcctgggt ctccccgcgc cgcccgagtg cggagccggt cactcccccc
     2041 gtgccgccct cggcggtatt gctccccgac atcatacgat cgttcaccat cgcggaccgc
     2101 tccacgccgg tctgggagcg cctcgagacg ctgctcgaat cgctcgagcg ctggcttgga
     2161 ttcgcggcgg tgagactgga cgtgatcgaa gacacgctca tcggcggcgg tgggccgggg
     2221 cgccgcgtgc acgtcgtggc cgaaaacgag ctgcgcgcga acgacgcgat cgcgcgcgtc
     2281 ttcgagtccg gcgcgcgtcg cgtcgtggag cggcgcgacg cggacgacga ggcccccggc
     2341 gcgccggagt cgtggggatc gctgggagta gcgccgatct tcgcgctggg cagcgtcgtg
     2401 ggggcgctgt cggtctacta tcccgcggga tcgccgcgcg atgccatgga cgccaatctc
     2461 gatctcgcgg ccggcgtggt gcgccaggcc atcgagttca accatcactt cgagagtttg
     2521 acctccatcg acgcgctcac cgggatctac aaccggcagt tcttcgaccg ccagatgccg
     2581 gtggaaatcg agcgcgcgat gcgatcggga agcgccttgt cgatgctggt gctggacctc
     2641 gacgacttca agcgcatcaa cgacgaattg ggtcacaaga agggtgacga agcgctggtg
     2701 gcggtggcgg agattattcg caagaacctg cgcaaggtcg acctcgcgtt tcgctacggc
     2761 ggcgaggagg tcgtgatact cttgccgggg acacccgagt tcgaagcggt gcacaccgcg
     2821 gaacgactgc ggcgcgtgat ccagcagcac cgcggatttc gcgacgcgcg tggtgcgccg
     2881 cgcgagatta cggtcagcgt cggggtggcg gtctatccgg acacggccaa gtcggccgac
     2941 gagctgttcg tccaggccga cgaagcgatg taccgcgcca agcagcgcgg caagaaccag
     3001 gtggtgctgt actcgggcgg ctagtggtat gcggggtggg ggtggtgcgg cgtggtgtgg
     3061 cttttctcat tgggcgtcgt cgccggcgtc tatcgatcgt tctctctcat tgtcatttta
     3121 atgctggccc tgctcgcgcg tgcggcgctc ttgcgcgcgg cgcacgcgtg ggtgtgcgtg
     3181 ccgctggcgc tcgcgtgcgg tgtcgtgggg tgggcgtcga tcgagcgcgt cgacgcggac
     3241 cggggcgcgg tcgagcgcgc ggcgcgggca tcggggggcg cggcgattcg gctgtgcggc
     3301 tgggtcgacg aatttccgca gtcggggcgc tacggcacga cgttctcttt ggcgacgcgc
     3361 gtcgaagggc ggccggtgcg actgtgggtg cgcgccgggc gcttcgacgt cggctacggc
     3421 gattcgctcg cgatcgacgc gcgcttggcg agcgcctcgc gcacgccgag cgtctttctg
     3481 gtctcgcgcg gactggcggg cgaagcgcgg gcgcgcttcg aagacgtgcg cgagatcgga
     3541 accccgcgcg gctgcccgct cctgagagag gtgttgtggc cgatgcaccg cgcggcgcgc
     3601 tgcaacctcg cgcgcgccct cggcggcgag gcggcgcttc cggtgggctt gctgctgggc
     3661 gagcgcggca tgctcgaccg ccgcgcccac gacgcggttc gcgggctcgg catcacgcac
     3721 ctgctggcgc tctcggggat gcacctgacg atgatcgcgg cgctggccgt gttcgcgacg
     3781 cgctggtcgc cgaggcgccg cgacacggtc gtcgcgctgg cgctatcgct ctacgtcggt
     3841 gtggtcggca acgtcgattc gctgacgcgt gcgtggtgga tggcgctctt gatcctcgcc
     3901 tcgcgcgcgt tggtgcggcc gccgcggccg ctcgatgcgc tcggaaaggc gcttttcctc
     3961 atgctcctgt gcgcgccgca cgcggtcttg tcggtcggtc tgcaattgtc gttcgtggcc
     4021 accttcgcgg tcctcgtgag tctcgagcgt ttgccctccg cgctgctgcg atcgccgccc
     4081 gcccgcgccc cgctgtggcg gcgcgcggga attcgtgccg gacaaggcgc cgcgatcgcg
     4141 ttcatggtga gcctggtggt cgaagtgttc atcgcgccgc tccaggtgca ccacttcggg
     4201 cggatcagcg tcgtgggtcc gctggccacc gtcgtgttct tgatcccggt gacggtgctg
     4261 cagggaatgg cgctggcggc gtccttcggc ctgccggtgg tcggggaacc gctggctgcg
     4321 ctgctggcct gggcctcgcg tgccacgtgc gaggccatcg tgctcgcggg gaccacggcg
     4381 ccggagccgc tggaggctcc ggagccccac tggttctggt actacgcggc cgtcctcgtg
     4441 gtctgcgccc ggccgcggcg cgcggtcgcg tgggcgatgg cggcggccgg aatcgcgctg
     4501 tcactccggc tgggcgccgg atcgtgacgg tacgccgcgt caaacgggcc ccttcggttt
     4561 tttccttcgc cgcccgcgcg cgcgatggta tagtgcgccg caaccgaagc ggaaggtgac
     4621 tgtgaccacc ggacgatgcc gagtggccgt tctggcctcc ggccgcggaa gcaacttcag
     4681 agcgctcgca gagcgctgcc aggacaagac gtttccggcc gaagtggcct gcctcatgac
     4741 cgacaaccga tccgcgggcg cgatcgagat cgcggacgcg ttcggtatcc cgtggcacgc
     4801 cgtggacgcg ggcgaaaagc ggggccggct caagccgggt gcggagtccg agatcgtggc
     4861 gcactgccag tcggcgcgca ccgatctcgt cctgctcgcg ggtttcatgc gcatactcga
     4921 ggacgcgccg ggcgggttgc tggattgttt ccgcagccgc gtgatcaacg tccacccgtc
     4981 gttgctcccg agcttcaagg ggctgcacgc gcagcggcag gccctcgagc atggggtcaa
     5041 ggtcgcgggg tgtacggtcc acttcgtcga cgcgtccgtc gacggcgggc ccatcgtcct
     5101 gcagtcggcg gtaccggtgc gcgacgacga cgacgtggaa tcgctctccg cgcgtattct
     5161 cgaggaagag caccgcgcgg ttacgcgggc cgtcgagttg ttcgcgcgcg gccagctcaa
     5221 ggtcgaagga agacgtgttc tggggacgga tgaaccatga tcaaagccat cggaagcacc
     5281 actgaactcg gactcaagcc cgatcgcgcc ggaaaagtgc gcgacgtatt tgacatcggc
     5341 gatcgcctgc tcatcgtctc gaccgaccgc ttgagcgcgt acgacgtcgt gctgccgaca
     5401 cggctgcccg gcaagggcgt cttgctgacc caaatcacgc tcggctggta cgagcacttt
     5461 ggacgttcgc tcgcgaccca cttcttgacc gccgaggtgt cggagtatcc cgctccgttt
     5521 cgcggcgtcg acgccctcgc cggtcgcagc atgctcgttg cgaaggcgaa gcgcttcgac
     5581 gtcgagtgcg tcgtgcgcgg ctacttgagc gggtcgggct ggaaggaata ccagcgcaat
     5641 cgctcggtgt gcggcatcgc gttgcccgac gggctcagcg agtccgcgga gctgcccgag
     5701 cccatcttta ccccggccac caaggccgat tccggccacg acgagaacat ctccttcgaa
     5761 cagatgtgcg agatcgtccc gcgtcgcgac gccgaagcgc tgcgcgcgca gagcatcgac
     5821 ctgtaccgcc gcggccgcga ctacgcccgc acgcgcggca tcctcctcgc cgacaccaag
     5881 ttcgagttcg gcgtgatcga cggccagatc aagttgatcg acgaggtgct cacaccggat
     5941 tcgtcgcgct tctggccggc cgaccagtac gcgcccggcc gcgcgcagga gagcttcgac
     6001 aaacaattcg tgcgcgacta cttggacgcg agcgggtggg accacaaccc gcccggaccc
     6061 gagctgcccg gcgacgtggt gacacgcacc atcgcgcgct acaaagaagc gcacgacctg
     6121 ttgttcccgg gccgaaacgt ggcgaggtac ctgtgagcaa gtcggctcgg aatcgtaccg
     6181 cgctgatcag cgttaccgac aaggcgggtc tgcccgagtt ggccgctgcg cttggcgacg
     6241 cgggacgatc
//
LOCUS       DASPGK010000119         9069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_734536, whole genome shotgun sequence.
ACCESSION   DASPGK010000119 DASPGK010000000
VERSION     DASPGK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734536"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VEC56_03425"
     CDS             <1..385
                     /locus_tag="VEC56_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV13235.1"
                     /translation="LSTHILPEVNLLCRRVLIIHRGRLIADARPEDLRAHGATALRVD
                     VEARASAATVSDLLRQTPGVASVEALPPAASGDARVRVTAAEGADPREAIAHALVRAG
                     MGLRSMSVATATLEDVFLELVTKEE"
     gene            387..1157
                     /locus_tag="VEC56_03430"
     CDS             387..1157
                     /locus_tag="VEC56_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV13236.1"
                     /translation="MHKVLAIYRREIAYYFQSPVAYAVITIFLLLAGYFFYNLLGYFN
                     LASMQAMHNPVQSRSLSLTASVMQPLLGNLSTVLLLLLPLVTMRLFAEERRSGAAELL
                     FTYPVSDAAVILGKFGAAVTVYLVMLAMTLPYPALLYHFGNPEPGPIVSGYLGVFLLG
                     VAFIAMGTFFSSLGDSQMVAAASTFGCGLLFLIIGWTTPFVSTTLGAVLEQLSLVHHL
                     DGFARGVIDTNDLVFYLGLTVFFLFLCARVLDSARWRS"
     gene            1165..2751
                     /locus_tag="VEC56_03435"
     CDS             1165..2751
                     /locus_tag="VEC56_03435"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HXV13237.1"
                     /translation="MHTPPVDRTSSRTLVWRVVAAVGLALFAVAALFFAMGSADARAW
                     GIPAALGAAMLVAAGAANARSVTAFLGRRESRRGADAFLSTLFLAAILVVVQATSMRR
                     SHTFDLTRNHRHTLSPQTTALLDSLDRDVEVTGFFRQASARRDGAEDLFALYAGRSHR
                     FRASVVDPDRQPDLARRLGASLDEVVVQADDARRVVRTVDEESLTNAILSVTRTGTRA
                     VYFVTGHGEKDPQVSDREGYSAVRHGLESQGYVVRAASLMGGAPVPDDCAVLVLAGPR
                     QDYFAEEVTAVRRYLDAGGSLLALIDPRVEVPRLAQLFARYHLGLVDAVVLDELALDA
                     GDRSFDATVAKVRRYESHPITRDFNYVTMFPRARPVVIAEDSVVAGLEARYLCISDDR
                     AWGEVDMESFAIGRATRDGADIAGPLPIAAAATRTPLSPAGVGAGSGKSRVVLVGDSD
                     FAGNTMYNVLGNSDFFLNAIAFLSEDENMIRIRPRRPLGESVYITERQGRMVFLVCLV
                     LLPLTPIVTGAVVVARRRRL"
     gene            2748..4082
                     /locus_tag="VEC56_03440"
     CDS             2748..4082
                     /locus_tag="VEC56_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXV13238.1"
                     /translation="MTLSRTSRVLFVLAAAAVAAFALLELPRRRAAEREMDHARRLFP
                     PFAASVDRVEIVRPGARVLLEARGTRWEVVEPVHDAAEYSRVATLLDALARAYVERNL
                     GPTDDDARFGLSPPVAVVTVSAAGDTLAYLELGAHTVDGAFAYARRDDRDIVLVPPAL
                     VSAAALPTDAFRDQNLVRFDPGEVEAFTVRRDGEAPVRWTRHRADAWFTVVAGDTVAG
                     DSVEVPAHLRRFRGMRVRAFVDPADTLGAFAHASGSVTFHKRAPAPAVTIRFAARSDS
                     SYWARTDGESRVIDVHGDVASALDASPARLRDRRLLQFDPRRARRIQVVTPDTSAVLV
                     RAGDAWALPNPALGRIDPDAATDFVRALRTLRYRSVVDGTPNDLEPPAFTLVIAGDGD
                     TILDELRGRPRAGEAAAWIVTSRSSRAISELAADDVEALADRLRRLRVAPRR"
     gene            4084..4953
                     /gene="amrB"
                     /locus_tag="VEC56_03445"
     CDS             4084..4953
                     /gene="amrB"
                     /locus_tag="VEC56_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXV13239.1"
                     /translation="MGSSRSVRTPAVAGMFYPEDPNVLARTVDALLAGAAAERPGTIE
                     RPRVLVAPHAGYTYSGAVAARAFALLDDSFHTVVLVGPSHVDAFDFTSVYDGDGYETP
                     LGVLEVDRAAARRIAANTTTIRISDRGHVAPRHARGEHGLEVMLPFLQRVCGSPRIVP
                     VVMGSQDWGACAELGRALAETAERERVLIVASSDLSHFYSYDDAVRLDTAFCELLATG
                     DAERLYEGVRAGRCEACGAGPVVASLVAANTWSDRRCRVLARINSGDVTGDRTRVVGY
                     ASAVVTASSRLPA"
     gene            4950..5510
                     /gene="amrA"
                     /locus_tag="VEC56_03450"
     CDS             4950..5510
                     /gene="amrA"
                     /locus_tag="VEC56_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HXV13240.1"
                     /translation="MTIDFVSRRTLLAIARHAIGSAVDPEQHSDAAAVDHALPVLAEL
                     RGAFVTLHQRGRLRGCIGRIEPDAPLRVLVPDVARAAALSDPRFPAVRADDLRTLVIE
                     ISLLSVLATVKDVDEIEIGRHGLVIAARGRRGLLLPQVAVEFGWSRAEFLSEVCAKAG
                     LAPDAWRDEGVRLQSFTAEVFSEQDE"
     gene            complement(5557..7056)
                     /locus_tag="VEC56_03455"
     CDS             complement(5557..7056)
                     /locus_tag="VEC56_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HXV13241.1"
                     /translation="MSVIEQEQLTSLIGEIEDYARGYGLDFFPVIFEMLDYEEINMIA
                     SYGGFPTRYPHWRFGMQYEYMQKSYGYGLHKIYEMVINNNPCYAYLLNSNQIVDQKLV
                     IAHVYAHCDFFKNNAYFRNTNRRMMDEMANHGNRIADYMSTYGRDVVEDFIDVCSSLE
                     NLIDPNALFEPDERREETASEEVSPARDVARLKSKSYMDPYINPPEYLEQQRRAMDEA
                     RANKQFPARPAKDVLRFLIEYAPLPNWKRDILCMIREEAYYFAPQAQTKIMNEGWASY
                     WHEKIMTQKALSASELVDFADHHSSTLAVHPGHLNPYKLGYELYHDIERRWDTGRFGK
                     EYDECDDIARKAAWRRDTGEGREKIFQVRAIYNDMTFVDEFLTEEFCHEHKLFTYSLN
                     ERNGRYEIADRDFRAVKEKLLFQLTNLGHPFIGVVDANFDNRGELYLKHRFDGVELRA
                     DYAKATMEALYRVWTRPVHVQTTVGEATVVLTYDGSTHRERALSPADAA"
     gene            complement(7059..8162)
                     /locus_tag="VEC56_03460"
     CDS             complement(7059..8162)
                     /locus_tag="VEC56_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HXV13242.1"
                     /translation="MIQRIDRDLNRFRQVVRGIVKKELRKFMVTGELVGRQGRDYVSI
                     PIRQIEIPTFRHETRRFGGVGQGDGDPGTPIGQAEGDGKGPAGDAPGDHMVEVEVALA
                     ELARIMAEELELPNIKPKSKDVIEAEHGKFTGIHKNGPESLRHFRRTFREALKRQIAS
                     GAYDFAHPVIVPIKDDKRYRARRTVHAPETNAVIFYLMDVSGSMGDEQKDVVRNESFW
                     INTWLRHQYKGVVTRFIVHDAEAREVDEHTFFHTRESGGTRISSAYQLAADLIESECP
                     PSEFNVYLFHFSDGDNWGGGDTQVCVKLLNERLLPAANLFCYGQVASPYGSGAFINDL
                     GGAFKGIDNVVLAEINSRDEIYDSIKKFLGKGR"
     gene            complement(8180..>9069)
                     /locus_tag="VEC56_03465"
     CDS             complement(8180..>9069)
                     /locus_tag="VEC56_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HXV13243.1"
                     /translation="HIAPHSIEMAAMWAVLTRLEQPKKAQLNLLQKLKLYDGKTMPGF
                     TEENVKELQNEAKREGLDGISPRYIQDKISNALVSDEADRCINPFMVLNELESGLQHH
                     SLINSDELRKRYRDLLAQVKDEYEDIVKNEVQRAISADEDAITKLCANYVDNIKAYTQ
                     KERVKNKYTGEDEEPDERLMRSIEEKIGIPENRKDDFRREIMNYIGALAIDGKTFDYK
                     TNARLQKALELKLFEDQKDSIKLTNLVSSVVDKETQAKIDIVKQRLIRNMGYCDICAT
                     DVLNFVASIFARGDVKRSS"
BASE COUNT         1256 a         3081 c         2990 g         1742 t
ORIGIN      
        1 cttgagcacg cacatcctgc ccgaggtcaa cctgctgtgc cggcgcgtgc tcattatcca
       61 ccgcggccga ttgatcgcgg atgcgcgccc cgaggacctg cgcgcgcacg gggcgaccgc
      121 gctgcgcgtc gacgtggagg cgcgcgcgag tgccgctact gtctcggatc tactgcggca
      181 aacgccgggc gtcgcgtcgg tcgaagcgct cccgcccgcg gccagcggcg acgcgcgcgt
      241 gcgggtgacg gcggccgagg gagccgaccc gcgcgaagcg atcgcgcacg cgttggtgcg
      301 cgccggaatg gggttgcgct cgatgtccgt cgccaccgcg accctcgagg acgtattcct
      361 cgaactcgtg acgaaggagg agtagcgtgc acaaggtgct cgccatctat cgccgggaga
      421 ttgcgtacta tttccagtcg ccggtcgcgt atgccgtgat cacgatcttc ctgctgctgg
      481 ctggatactt cttctacaac ctgctgggct acttcaacct ggcgtcgatg caagccatgc
      541 acaacccggt gcagtcgcgt tcgctgagcc tgaccgcctc ggtgatgcaa ccgctgcttg
      601 gcaatttgag caccgtgctg ctcctcttgc tccccctcgt caccatgcgt ctcttcgccg
      661 aggagcgtcg cagcggcgcg gccgagttgc tgttcaccta tccggtttcc gacgccgccg
      721 tgattctcgg caagttcggg gcggcggtca ccgtgtacct cgtgatgctg gccatgacgc
      781 tcccctaccc cgcgctgctc tatcatttcg gcaaccccga gccggggccc atcgtctcgg
      841 gatacctcgg cgtgtttctg ttgggcgtcg ccttcattgc gatggggacc tttttttcgt
      901 ccctgggcga cagtcagatg gtggcggcgg cctccacctt cgggtgcgga ctcctgtttc
      961 tgatcatcgg ttggacgacc ccgttcgtct cgaccacact gggcgcggtc ctcgaacaac
     1021 tctccttggt ccaccacttg gacggcttcg cgcgcggcgt catcgatacc aacgacctcg
     1081 tgttctatct cggtctcacc gtcttcttcc tcttcctgtg cgcgcgcgtg ctcgattcgg
     1141 cgcgctggcg gagttgacgg gcgcatgcac acccctccgg tcgaccgcac ttcctcgcgc
     1201 acgttggtct ggcgcgtcgt cgccgcagtg gggctcgcgc tgttcgcagt cgcggcgctg
     1261 ttcttcgcga tggggagcgc cgacgcgcgc gcgtggggaa tcccggccgc gctgggtgcg
     1321 gccatgctgg tggccgccgg cgccgccaac gcgcgctcgg tgacggcgtt cctcggccgg
     1381 cgcgaatcgc gccggggcgc ggacgcgttt ctctcaaccc tattcctggc cgcgatcctg
     1441 gtggtcgtcc aggcgacgtc gatgcgccgc tcgcacacct tcgatctgac gcgcaaccat
     1501 cgtcatacgc tctcgccgca gaccacggcg ctgctcgatt cgctcgaccg cgacgtcgag
     1561 gtgacgggat tcttccgaca ggcctccgcc cggcgcgacg gcgcggagga tctctttgcg
     1621 ttgtacgcgg gccggtcgca tcgctttcgc gcctccgtcg tcgatcccga ccgccagccc
     1681 gatctcgcgc ggcgcctggg cgcgtcgctc gacgaggtgg tcgtgcaggc ggacgacgcc
     1741 cggcgcgtcg tccgcaccgt cgacgaggag agcctgacca atgcgattct ctccgtcacg
     1801 cgaacgggaa cgcgcgcggt gtacttcgtt acgggacacg gcgagaagga cccccaggtc
     1861 tccgaccgcg aggggtactc ggcggtgcgg cacggcctcg aatcccaggg ctatgtggtg
     1921 cgcgccgcct cgctcatggg cggcgcgccg gtgccggacg actgcgcggt gctcgtcctc
     1981 gcgggcccgc gccaagacta cttcgccgag gaagtcacgg ccgtccggcg ctacctcgac
     2041 gccggcggct cgctgctcgc gttgatcgac ccccgggtcg aagtaccgcg gcttgcgcag
     2101 ctcttcgcgc gctatcacct ggggttggtc gacgcggtcg tgctggacga gctcgcgctc
     2161 gacgccggcg accgctcttt cgacgccacc gtggccaagg ttcggcgcta cgaatcgcac
     2221 ccgatcacgc gcgacttcaa ctacgtgacc atgttccctc gcgcgcgccc ggtcgtgatc
     2281 gccgaggact cggtggtggc cggactcgaa gcgcgctatc tgtgcatctc cgacgaccgc
     2341 gcgtggggcg aggtcgacat ggagagcttc gcgatcgggc gcgcgacgcg cgacggcgcc
     2401 gatatcgccg gcccgcttcc catcgccgcc gcggccacgc gcactccgct ctcccccgcc
     2461 ggagtcggag ccggcagcgg caagagccgc gtggtcctgg tgggtgactc ggatttcgcc
     2521 ggcaacacga tgtacaacgt gctcggcaac tccgacttct ttctgaacgc aatcgcgttc
     2581 ctatccgaag acgagaacat gattcgtatt cgcccgcggc gcccgttggg cgagagcgtc
     2641 tacatcaccg agcggcaggg gcgcatggtc ttcctcgtgt gcctggtctt gctcccgctc
     2701 acacccatcg tgacgggcgc ggtggtggtg gcgcgccgcc gccggctatg acgctctcgc
     2761 gcacctcgcg cgtcctcttc gtgctcgcgg ccgcggccgt ggcggcgttc gcgctgctcg
     2821 agttgccgcg ccgccgggcg gcggagcgag aaatggacca cgcgcgccgc ctcttcccgc
     2881 ccttcgcggc gtcggtcgac cgcgtcgaga tcgtgcgtcc cggagcgcgc gtgctcctcg
     2941 aggcgcgcgg cacgcgttgg gaagtggtcg agcccgtgca cgacgcggcc gagtactcgc
     3001 gcgtggccac gctgctcgac gcgctcgcgc gtgcctatgt ggagcgcaat ctcggtccca
     3061 ccgacgacga cgcgcgcttc ggtttgtcac ccccggtcgc cgtcgtgacc gtcagcgcgg
     3121 ccggcgacac cctcgcctac ctggagctgg gcgcccacac cgtcgacggc gcctttgcgt
     3181 acgcgcgccg cgacgaccgt gacatcgtgc tggttccacc cgcgctcgtg agcgcggccg
     3241 cacttcccac cgacgcgttt cgcgaccaga acctcgtgcg cttcgacccc ggcgaagtcg
     3301 aggccttcac cgtacgccgc gacggcgagg cgccggtgcg atggacgcga cacagagccg
     3361 acgcgtggtt cacggtggtc gcgggcgaca ccgtcgccgg cgactcggtc gaggtcccgg
     3421 cccacttgcg ccgatttcgc ggcatgcggg tgcgcgcgtt cgtcgatccg gcggacacgt
     3481 tgggcgcgtt cgcgcacgcg agcggaagcg tcacctttca caagcgcgcc cccgcgcccg
     3541 cggtgacgat tcgcttcgcc gcgcgttccg acagttcgta ctgggctcgt accgacggag
     3601 agtcgcgtgt gatcgatgtg cacggggatg tcgcgagtgc cctcgacgcc tcccccgcgc
     3661 gtctccgcga ccggcgtttg ctgcagttcg acccgcgtcg cgcgcggcgc atccaggtcg
     3721 tgacgccgga tacctcggcc gttctggtgc gcgccggcga cgcgtgggcg ctgcccaacc
     3781 cggccctggg tcgcattgac cccgatgccg ccaccgactt cgtgcgagcc ctgcgcacgc
     3841 tgcgctaccg ctccgtggtc gacggtacgc cgaacgacct ggaaccgccg gcctttaccc
     3901 ttgtgatcgc cggcgacggt gatacgattc tcgacgagtt gcgcggacgg ccgcgcgcgg
     3961 gcgaggcggc ggcgtggatc gtcaccagcc gctcgtcgcg cgcgatatcg gagctggctg
     4021 cggacgacgt cgaggcgctg gcggaccgtc tgcgacgact gcgcgtcgcg ccccggaggt
     4081 agcatgggtt catcgagatc ggtgcgcaca cccgcggtgg ccgggatgtt ctaccccgaa
     4141 gatccaaacg tactcgcgcg cacggtcgat gcactcctgg ccggcgcggc ggccgagcgc
     4201 cccggaacga tcgaacgccc gcgcgtgctc gtcgcaccgc acgcgggata cacgtactcc
     4261 ggcgcggtcg ccgcgcgcgc cttcgcgctg ctcgacgact cgttccacac cgtcgtcttg
     4321 gtgggaccgt cacacgtgga tgcgttcgac ttcacgtcgg tgtacgacgg cgacgggtac
     4381 gaaaccccgc tcggtgtgct ggaggtcgac cgcgctgccg cgcgtcgaat cgccgccaac
     4441 accacgacca ttcgtatctc tgaccgcgga cacgtcgcgc cgcgccacgc gcgcggtgag
     4501 cacggcctcg aagtcatgct gcccttcctg cagcgcgtgt gcggaagccc gcgcatcgtg
     4561 cccgtagtca tgggaagtca ggattggggt gcgtgcgccg agctgggacg cgccctcgcg
     4621 gagaccgcgg agcgcgagcg agtgctcatc gtcgcgagct ccgacctctc gcatttctat
     4681 tcgtacgacg acgcggtgcg tctcgacacc gcgttctgcg agctgctggc aaccggcgac
     4741 gccgagcgcc tctacgaggg tgttcgcgcc ggtcgctgcg aagcctgcgg tgcgggcccg
     4801 gtggtggcct ccctggtcgc cgcgaacacg tggtcggacc ggcgctgccg cgtgctcgcc
     4861 cggatcaact ccggggacgt caccggcgac cgcacccgtg tggtcggcta cgcgtcggcc
     4921 gtcgtcaccg cgtcctcgag gctgcccgca tgaccatcga cttcgtgtct cgacgaacgc
     4981 tgcttgcgat cgcccgccac gcgatcggct cggccgtcga ccccgagcaa cattcggacg
     5041 ccgccgcggt ggatcacgcg ctgcccgtcc tcgccgagct gcgcggggcg ttcgtgacgc
     5101 tccaccagcg cggacgcctg cgcgggtgca tcggccgtat cgagcccgat gccccgctgc
     5161 gcgtgctcgt tcccgacgtc gcgcgcgcgg ccgcgctctc cgacccccgg tttcccgccg
     5221 tgcgcgccga cgacctgcgc acgctcgtga tcgagatctc gctcctctcg gtgctcgcaa
     5281 cggtcaagga cgtggatgag atcgagatcg gtcggcacgg gctcgtgatc gccgcgcggg
     5341 gtcgtcgcgg gctcctcctg ccccaggtcg cggtggaatt cggctggtcg cgcgcggagt
     5401 tcctctccga agtgtgcgcc aaggccggcc tcgccccgga cgcgtggcga gacgaagggg
     5461 ttcgcctgca gtcgttcacc gccgaggtgt tttcggagca agacgagtag tgcgctgcgc
     5521 ggcacgagcg cgcgatgggc gcgccctcgg gtaagcctag gccgcgtcgg cgggcgagag
     5581 tgcgcgctcg cggtgggtcg atccgtcgta ggtgagaacc accgtggctt cgcccaccgt
     5641 ggtttgcacg tgtacgggac gcgtccacac ccggtacagc gcttccatgg tggccttggc
     5701 gtaatccgcg cgcagttcca ctccgtcgaa acggtgcttg aggtatagct cgccgcggtt
     5761 gtcgaagttc gcgtcgacga cgccgatgaa ggggtggccg aggttggtga gctggaacag
     5821 cagcttctcc ttgaccgcgc gaaaatcgcg gtccgcgatc tcgtagcgcc cgttgcgctc
     5881 attgagcgag tacgtgaaca gcttgtgctc gtggcagaat tcttcggtca agaactcatc
     5941 cacgaacgtc atgtcgttgt agatcgcgcg cacctggaag atcttctcgc gcccctcgcc
     6001 cgtgtcccgc ctccaggccg ccttgcgcgc gatgtcgtcg cactcgtcgt attccttgcc
     6061 gaagcgcccc gtgtcccagc ggcgctcgat gtcgtgatag agctcgtagc cgagcttgta
     6121 gggattcagg tgacccgggt ggaccgccag cgtcgacgag tggtgatcgg cgaagtcgac
     6181 cagctcgctc gccgacagtg ccttctgggt catgatcttt tcgtgccagt aagacgccca
     6241 gccctcattc atgatcttgg tctgggcctg gggcgcgaag tagtaggcct cttcgcgaat
     6301 catgcacagg atgtcgcgct tccagttggg caggggcgcg tactcgatca agaagcgcag
     6361 gacgtccttg gccggccggg ccggaaactg tttgttcgcg cgcgcttcgt ccatggcgcg
     6421 acgctgttgc tccaagtatt cgggagggtt gatgtacggg tccatgtacg acttgctctt
     6481 gagacgagcg acgtcgcgcg ccggcgacac ctcctccgac gcggtttcct cgcgccgctc
     6541 gtcgggctcg aacagggcgt tgggatcgat caggttctcc agcgacgagc acacgtcgat
     6601 gaagtcctcc accacgtcgc ggccgtaggt cgacatgtag tccgcgatgc ggttgccgtg
     6661 gttcgccatc tcgtccatca tgcggcggtt ggtgttcctg aagtaggcgt tgttcttgaa
     6721 gaagtcgcag tgggcgtaga cgtgcgcgat caccagcttc tggtcgacga tctggttgga
     6781 gttcaggaga taggcgtagc atgggttgtt gttgatcacc atctcgtaga tcttgtgcag
     6841 cccgtagccg tagctcttct gcatgtactc gtactgcata ccgaagcgcc agtgcgggta
     6901 gcgggtcgga aagccgccgt acgaggcgat catgttgatc tcctcgtagt ccagcatctc
     6961 gaagatcacg ggaaagaagt cgagaccgta gccccgcgcg tagtcttcga tctcgccgat
     7021 cagcgacgtc agctgctctt gctcgatgac cgacatgctc accgcccctt ccccaagaat
     7081 ttcttgatcg aatcgtagat ctcgtcgcgg ctgttgatct ccgctagaac cacgttatca
     7141 atgcctttga aggcgccgcc cagatcgttg ataaaggctc cgctgccata cgggctcgcc
     7201 acctggccgt agcagaacag gttcgcggcc ggcaacaggc gctcgttcag gagcttcacg
     7261 cacacttggg tatcgccgcc gccccaattg tcgccgtccg agaagtggaa caggtagacg
     7321 ttgaactcgc tgggcggaca ctcggactcg atcaggtcgg ccgccagctg gtaggccgac
     7381 gagatgcgcg tgccgccgct ttcgcgcgtg tgaaaaaagg tatgctcgtc gacttcgcgc
     7441 gcctccgcgt cgtggacgat gaagcgcgtc accacgccct tgtactggtg gcgtagccag
     7501 gtgttgatcc agaaggattc gttgcgcacg acgtccttct gttcgtcccc catcgacccg
     7561 gacacgtcca tgagatagaa gatgaccgcg ttggtctcgg gcgcgtggac ggtcctgcgc
     7621 gcgcggtagc gcttgtcgtc tttgatgggc acgatgacgg ggtgcgcgaa gtcgtacgcg
     7681 ccggacgcga tctggcgctt cagggcctcg cgaaacgtgc ggcgaaaatg gcgcagcgat
     7741 tcggggccgt tcttgtggat gccggtgaac ttgccgtgct cggcctcgat gacgtctttg
     7801 ctcttgggct tgatgttggg gagctcgagt tcctcggcca tgatgcgggc gagctcggcc
     7861 agcgccactt cgacctcgac catgtggtcg ccgggtgcgt ccccggccgg ccccttgccg
     7921 tcgccttccg cctggccgat cggggtcccc ggatcgccgt caccctggcc caccccgccg
     7981 aagcggcgtg tttcgtggcg aaacgtggga atctcgatct gccggatggg aatggagacg
     8041 tagtcgcgtc cctggcgccc caccagctcg cccgtcacca tgaacttgcg gagctctttc
     8101 ttgacgatgc cccgcaccac ctgacggaac cgattcaggt cccggtcgat gcgctggatc
     8161 atacgcgcct cccggaccgc tagctcgatc gcttgacgtc gccgcgcgcg aagatgctgg
     8221 ccacgaagtt caagacgtcg gtcgcgcaga tgtcgcagta acccatgttg cgaatcaagc
     8281 gctgcttgac gatatcgatc ttggcctgcg tctccttgtc gaccacactc gacaccaagt
     8341 tggtgagctt gatcgagtcc ttctggtcct cgaacagctt gagctcgagc gccttctgca
     8401 gacgcgcgtt ggtcttgtag tcgaaggtct tgccgtcgat cgcgagcgcc ccgatgtagt
     8461 tcatgatctc gcgccggaag tcgtccttgc gattctcggg gatgccgatc ttctcttcga
     8521 tcgaccgcat caggcgctca tcgggttctt cgtcctcgcc cgtgtacttg ttcttgacgc
     8581 gctccttctg cgtgtaggcc ttgatgttgt cgacgtagtt cgcgcacaac ttggtgatcg
     8641 cgtcctcgtc ggccgagatg gcgcgctgga cctcgttctt cacgatatcc tcgtactcgt
     8701 ccttgacctg ggccaacagg tcgcggtagc gcttgcgcag ttcgtcggag ttgatcagcg
     8761 agtggtgctg cagccccgac tccagctcgt tgaggaccat gaaggggttg atgcagcggt
     8821 cggcctcgtc gctcaccagc gcgttggaga tcttgtcctg gatgtagcgc ggcgagatcc
     8881 catcgaggcc ctcgcgcttc gcctcgttct gcagctcctt gacgttctcc tccgtgaacc
     8941 cgggcatcgt cttgccgtcg tagagcttca gcttctggag caggttgagc tgggccttct
     9001 tgggctgctc caggcgcgtc agcacggccc acatcgccgc catctcgatg gagtgggggg
     9061 cgatgtgct
//
LOCUS       DASPGK010000120         5952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1671422, whole genome shotgun sequence.
ACCESSION   DASPGK010000120 DASPGK010000000
VERSION     DASPGK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671422"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /gene="purF"
                     /locus_tag="VEC56_03470"
     CDS             <1..1331
                     /gene="purF"
                     /locus_tag="VEC56_03470"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXV13244.1"
                     /translation="FLGLYALQHRGQEGAGIVSTDGDRMYEHKGMGLVAQVFDEESIA
                     RLKGNAAVGHNRYSTTGLPRVANIQPLLVDCRVGKLALAHNGNLVNARELRQHMEASG
                     SIFNTTMDSEVILHLIARSKEEDLDSMIVDAVQQLRGAFSVLLLTKDSLIAVRDPQSF
                     RPLCLGRKGDATIITSESCALDIIGAEYVRDIEPGELVIVDERGIRSRKAFEPQQQAK
                     CIFEFIYFSRPDSKVFGENVDKVRRKLGVVLAKNHPVDADIVISVPDSSNTSSLGFAQ
                     SSGIPFEIGLIRNHYVGRTFIQPSQRLRDGTVRLKFNTVDGVLKGKRVVVVDDSIVRG
                     STMKKLVVMLRKAGAKEVHLRIASPPVKFPCFYGIDMPSRSELIASSTSVEGIRQYLG
                     VESLEYLTIADLRSVVDDPDRFCNACFSGEYPIPMASDLDKSVLEDTAVA"
     gene            1437..2117
                     /locus_tag="VEC56_03475"
     CDS             1437..2117
                     /locus_tag="VEC56_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HXV13245.1"
                     /translation="MKIRIQFEKLGTVRFTSHKDVVRMFERAFAAAAVPVAWSEGFHP
                     HMRMSFGPPLRTGWESREEYMDITMEDASSAVMDGFAAAVNEKLPEGLVIRRVVAIDD
                     RTPKLASDMSAVSLEVSVAADDAGTDADAVSSRISNRFLNNNHAETEPRVSEADVVRS
                     QDALRIRYTSTMQNGRIVTPEDVVSAAIGDPESFRVPIKVVRLAQFVARGDRLVSPLD
                     EGVVQASV"
     gene            2114..3781
                     /locus_tag="VEC56_03480"
     CDS             2114..3781
                     /locus_tag="VEC56_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXV13246.1"
                     /translation="MKEVLINSTSQEVRAAIVEEGELVEFMVERAATRRLVGDIYLGR
                     VNAILPGIQAAFVDIGYEKAGFLHASDLVPDTESMESDVDVDDDDDHGRRGRGRGRDD
                     EDDRDDRNDRNGRDNRRGGGRGRDDRGGRGGGGRREPRDRPVVQPIEKMLTKGQEVLV
                     QVTKEAIGTKGPRLTTQISIPGRHVVYMPNSDYLGISRRIESRQERARLRTLISKKKP
                     PNCAVIARTACEGVEDKQIVADINYLHKLWQEIRRRAEKAQAPELVHEEVGMVVGMVR
                     DLIAEDIDKIWMDNEREYRRLVRHLRQVSPELASRTLLFRDKSPIFEKYNIENEIDKT
                     LERKVWLKKGGYLVFDQTEAMVVVDVNTGRFVGKRDQEETILETNLLAAREVPRQLRL
                     RDIGGIIVIDFIDMESEANKKKVLTELRNHLRRDRSRAKAFAISDLGLVEISRKRVRP
                     SLLHFLSEECPYCRGTGKVLSFDTLANKVERQIHRIGQRTKERRVQLRVNTSFGVFLE
                     EQRAGMLDSLEKQYRMRIEIQDDPRLHREDFKLVSLSNFRDLVAEAG"
     gene            3821..4525
                     /locus_tag="VEC56_03485"
     CDS             3821..4525
                     /locus_tag="VEC56_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV13247.1"
                     /translation="MNLKDARILVTGGSLGIGKAAAQVLVEAGARVAITARHEERLHK
                     TANAIGAVAIVADAGSPRDVDRTFETVRERLGGLDALINNAGIGEFTDMESVTFEEFD
                     KIFRVNVYGAALMGARAAAMFKAQKSRGHIINVGSTAGLKGFAGGTVYAASKFALRGM
                     TECWQAELRKHNIRVTLIAPSEVATAFGREDGKERTAPPNKLTPMEIAHAIKSVLELD
                     DRAMIPELSVWATNPW"
     gene            complement(4644..>5952)
                     /locus_tag="VEC56_03490"
     CDS             complement(4644..>5952)
                     /locus_tag="VEC56_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV13248.1"
                     /translation="NGEKVLIGKDDAYYVVDVKEDQKLEKKMPSGELAMTVDPRAEWK
                     QIFNDVWRFERDYFYDPGMHGVDWNRMRTQYGKLLDDAVTRWDVNFVIGELIAELNAS
                     HTYRGGGDEEQAQEVAVGYLGVDWELANGAYRIKKIIRGAAWDTEVRSPLDMSGIDVN
                     EGDYVLAVNGRALDAAKGPWAAFAGLAKRPVVLTVNSKPSTDGARSVLVETLDSETRL
                     RHLAWIEGNRKRVDEATGGKIGYIYVPSTGRDGQTELVRQFAAQFDKQGLIVDERFNS
                     GGQIPDRFVELLNRKPLAFWAVRDGRTWQWPPIANFGPKVMLINGWSGSGGDAFPDYF
                     RKAGAGPLIGTRTWGGLIGITGAPTLIDGGQVTVPTFRMYDPDGTWFKEGYGVDPDIE
                     VPEDYGQLAKGVDVQLERGIQEVLRLLAASPSPMVKQPAFEKR"
BASE COUNT         1094 a         1915 c         1940 g         1003 t
ORIGIN      
        1 cgttcctcgg tctgtatgcc ctgcagcacc gcggtcaaga gggcgccggc atcgtatcca
       61 ccgacggcga ccggatgtac gagcacaaag ggatgggact cgtcgcacag gtgttcgacg
      121 aggagtccat cgctcggctg aagggaaatg ccgccgtcgg ccacaaccgc tattcgacga
      181 ccggcctgcc acgcgtggcg aacatccagc cgctcttggt ggactgccgg gtcgggaaac
      241 tcgccctcgc gcacaacgga aacctcgtca acgcccgcga gcttcgccag cacatggaag
      301 cgtcggggtc catcttcaat accacgatgg acagcgaggt cattctccac ctcatcgcgc
      361 gcagcaagga agaggatctc gactccatga tcgtcgacgc ggtgcagcag ctgcggggtg
      421 cgttctccgt cctcctactg acgaaggact cactcatcgc cgttcgcgac ccgcagtcgt
      481 tccggccgct ctgtttgggc cgtaagggtg atgcgaccat catcaccagc gaatcctgcg
      541 cgctggacat catcggcgcc gaatacgttc gagacatcga gccaggggaa ctcgtcatcg
      601 tagacgagcg ggggattcgg tctcgaaagg cgttcgaacc ccagcagcag gcgaagtgca
      661 tattcgagtt catctacttc tcgcgtccgg acagcaaggt gttcggggaa aacgtcgaca
      721 aggttcgtcg caagctgggc gtggtcctgg ccaagaacca tcccgtcgac gccgacatcg
      781 tcatctctgt gccggactcg agcaatacgt cgagtttggg attcgctcaa tcctcgggca
      841 ttccgttcga gatcgggctg attcggaacc actacgtcgg gcgtacgttc atccagccca
      901 gccagcgtct ccgtgatggg acggtccggc tgaagttcaa cacggtggac ggtgtgctca
      961 agggaaaacg ggtggtggtg gtggacgact cgatcgtccg tggttccacg atgaagaaac
     1021 tcgttgtcat gctccgaaag gccggtgcga aggaagtcca cctgcgcatc gcgtctcccc
     1081 ccgtcaagtt cccctgcttc tacgggatcg atatgccgtc gcgaagcgag ctgatcgcgt
     1141 cgtcgacgtc cgtcgaaggc atccgccagt acctcggggt cgaaagcctc gagtacttga
     1201 ccatcgcgga ccttcgctcg gtcgtggacg acccggatcg attctgcaac gcgtgcttca
     1261 gcggcgagta tccgatcccg atggcgtcgg acctggacaa gtccgtgctc gaagacaccg
     1321 ccgtcgcctg acggttccaa ggggcttcgc gcgcgcgact gccgaacggc tcttttgagg
     1381 gccctggcgg ttcgggtcgc ttgaaacaac aacgcgcggg ttgtgccgcg cacagcgtga
     1441 aaatcaggat ccagttcgag aagctgggaa cggttcgctt tacgtcccac aaggacgtcg
     1501 ttcgcatgtt cgagcgcgcc ttcgccgcgg ccgcagttcc ggtcgcgtgg agcgaagggt
     1561 tccacccgca catgcgcatg tccttcggtc cgcctttgcg gaccgggtgg gagagccgcg
     1621 aagagtacat ggacatcacc atggaggatg cgtcctccgc cgtgatggac ggcttcgcgg
     1681 ccgccgtgaa cgaaaaactg cccgaagggc ttgtgatccg gcgcgtggtc gcgatcgacg
     1741 accgcacgcc caagctcgcg tcggacatga gcgccgtatc gctcgaagta tccgtcgcgg
     1801 cggacgacgc cgggacggac gcggacgccg tttccagccg catctcgaat cggttcctca
     1861 acaacaacca cgccgagacc gaaccccggg tcagcgaggc ggacgtcgtt cgatcgcagg
     1921 acgcgctccg catccgctac acgtcgacca tgcaaaacgg tcgcatcgtc accccggagg
     1981 acgtggtctc ggccgcgatc ggagaccccg agtcgtttcg cgtgcccatc aaagtggtgc
     2041 gtctcgcaca attcgtggcc cgcggcgacc ggctggtttc tccgctcgac gaaggagtcg
     2101 tgcaagcaag cgtatgaaag aagttctgat caattcgacc tcgcaggagg tccgggccgc
     2161 catcgtcgaa gagggcgagc tggtcgagtt catggtggag cgcgccgcca cgcggcgcct
     2221 ggtgggcgac atctacctgg gtcgcgtgaa cgcgattctc cccggcatcc aggccgcgtt
     2281 cgtcgacatc ggctacgaga aggcggggtt cctccacgcg agcgacctgg tgccggacac
     2341 cgagtcgatg gaaagcgacg tcgacgtcga cgacgacgat gaccacgggc gccgcggccg
     2401 gggccgcggt cgcgacgacg aagacgaccg cgacgaccgc aacgatcgta acgggcgcga
     2461 caaccggcgc ggcggcggac gaggtcgcga cgatcgcggg ggccgcggag gcggggggcg
     2521 gcgcgagccg cgcgaccggc ccgtcgttca gcccatcgag aagatgctga ccaagggcca
     2581 ggaggtcctg gtccaggtga cgaaggaggc catcggcacc aagggcccgc gcctcaccac
     2641 gcagatctcc attccgggtc gtcacgtggt ctacatgccc aacagcgact acctcggcat
     2701 ctcgcggcgt atcgagtccc ggcaggagcg cgcgcgtctg cgcacgctca tttcgaagaa
     2761 gaagccgccc aactgcgccg tcatcgcgcg caccgcgtgc gaaggcgtgg aagacaagca
     2821 gatcgtcgcc gatatcaatt atctgcacaa actctggcag gaaatccgcc gccgcgccga
     2881 aaaggcgcag gcgcccgagt tggtgcacga ggaagtcggc atggtggtcg gcatggtgcg
     2941 cgacttgatc gcggaggaca tcgataaaat ctggatggac aacgagcgcg agtatcgccg
     3001 gctggtccgt cacctgcgac aggtgagccc cgagctcgcc tcgcgcacgc tgttgtttcg
     3061 cgataagtcg cccatcttcg aaaagtacaa catcgagaac gaaatcgata agacgctcga
     3121 gcgcaaggtg tggctcaaga agggcggcta cctcgtgttc gaccagaccg aagccatggt
     3181 cgtggtcgat gtcaatacgg ggcgcttcgt cggcaagcgc gaccaggagg agaccatcct
     3241 cgagaccaac ctgctggcgg cgcgcgaggt gcccaggcag ctgcgcctgc gcgacatcgg
     3301 cggcatcatc gtcatcgact tcatcgacat ggagagcgag gccaacaaga agaaagtgct
     3361 gaccgagctg cgcaatcatt tgcggcgaga ccgctcgcgc gcgaaggcgt tcgcgatcag
     3421 cgacctggga ctggtcgaaa tcagtcgcaa gagggtgcgg ccgtctttgc tgcatttctt
     3481 gagcgaggag tgcccgtact gccgcggcac cggcaaggta ctctcgttcg ataccctggc
     3541 caacaaggtc gagcggcaga ttcaccgcat cgggcaacgc accaaggagc gccgcgtgca
     3601 gctgcgcgtc aacacctcct tcggcgtgtt tctggaagag cagcgcgccg gcatgctcga
     3661 ctcgctcgaa aagcaatacc gcatgcgcat cgagatccag gacgacccgc ggctgcaccg
     3721 cgaggacttc aagctggtct cgctgtcgaa cttccgcgac ctggtggccg aggcggggta
     3781 gcaccgcgcc gcaggcgcgg cgtgaacacg ggggtgtgcg atgaatctga aagacgcgag
     3841 aattctcgtc acgggcggga gcctgggcat cggcaaggcc gcggctcagg tcctggtgga
     3901 agccggcgcg cgcgttgcga tcaccgctcg ccacgaagag cgcctacaca agacggcgaa
     3961 cgcgatcggt gccgtcgcca tcgtggcgga cgcgggcagt ccgcgcgacg tcgatcggac
     4021 cttcgagacg gtgcgcgagc gcctgggagg gctcgacgcc ctgatcaaca acgcgggcat
     4081 cggcgagttc accgacatgg aatcggtgac gttcgaggag ttcgacaaaa tcttccgcgt
     4141 gaacgtctac ggcgcggcct tgatgggcgc gcgcgccgcg gccatgttca aggcgcagaa
     4201 gtcccgcggg cacattatca atgttggctc gaccgcgggg ctcaagggct tcgccggcgg
     4261 caccgtctac gcggcgtcca agttcgcgct gcgcgggatg acggagtgct ggcaggcgga
     4321 actgcgcaag cacaacatcc gcgtgaccct gatcgccccc agcgaagtcg ccacggcgtt
     4381 cgggcgcgaa gacggcaagg agcgcaccgc gccgcccaac aagctcacgc cgatggagat
     4441 cgcgcacgcg atcaagtcgg tactcgaact cgacgaccgc gcgatgatcc cggaattatc
     4501 ggtatgggcg acgaatccgt ggtgagcgcg tcatcgcacc ccgccgttgt ggtgggcttg
     4561 tgaattcatc cccaaactcc cacactggcg ataaacaatc atccactccg gatgaggacc
     4621 ggcgtcgcca gacggcgcgg gccctaccgc ttctcgaagg ccggctgctt caccatcggt
     4681 gacgggctcg cggcgagcaa gcgaagcact tcctgaattc cgcgctcgag ctggacgtcg
     4741 acacccttcg cgagctggcc gtagtcctcg ggcacttcga tgtcggggtc cacaccgtag
     4801 ccctccttga accacgtgcc gtcggggtcg tacatgcgga aggtcggcac ggtgacttgg
     4861 ccgccgtcga tcagcgtcgg cgcgccggtg atcccgatca aaccgcccca cgtgcgcgtc
     4921 ccgatgagcg gtcccgcgcc cgccttgcgg aagtagtcgg gaaaggcgtc gccgcccgaa
     4981 ccgctccagc cgttgatcag catcaccttg ggaccgaagt tcgcgatggg cggccactgc
     5041 cacgtgcgcc cgtcgcgcac ggcccagaac gcgagcggct tgcggttcag taattcgacg
     5101 aagcgatcgg gaatctgacc gccgctgttg aagcgctcgt cgacgatcaa tccctgcttg
     5161 tcgaactggg ccgcgaactg gcgcacgagc tcggtttgcc cgtcgcgccc ggtgctgggc
     5221 acgtagatgt agccgatctt gccgccggtc gcctcgtcga cgcgcttgcg gttgccctcg
     5281 atccacgcga gatggcggag tctcgtctcg ctatcgaggg tttcgaccag caccgaacgc
     5341 gcgccgtccg tcgacggctt cgagttgacg gtgaggacga cgggtctctt cgcgaggccc
     5401 gcgaacgcgg cccacggacc tttggcagcg tcgagtgcgc gcccgttgac ggcgagcacg
     5461 tagtcgcctt cgttcacgtc gatgcccgac atgtcgagcg gcgagcggac ttcggtgtcc
     5521 cacgcggcgc cgcggatgat cttcttgatc cggtaggcgc cgttcgcgag ctcccagtcc
     5581 acgcccaggt agccgaccgc gacctcctgg gcctgttcct cgtcgccgcc gccgcggtag
     5641 gtgtgcgacg cgttgagctc ggcgatcaac tcgccgatga cgaagttcac gtcccagcgc
     5701 gtcaccgcgt cgtcgaggag cttcccgtac tgcgtgcgca tgcggttcca gtcgacaccg
     5761 tgcatgccgg ggtcgtagaa gtagtcgcgc tcgaagcgcc atacgtcgtt gaagatctgc
     5821 ttccactcgg cgcgcgggtc gaccgtcatc gcgagctcgc ccgacggcat cttcttctcg
     5881 agcttctggt cttccttgac gtcgacgacg tagtacgcgt cgtccttgcc gatgagcacc
     5941 ttctcgccgt tc
//
LOCUS       DASPGK010000121         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_500663, whole genome shotgun sequence.
ACCESSION   DASPGK010000121 DASPGK010000000
VERSION     DASPGK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500663"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..875)
                     /locus_tag="VEC56_03495"
     CDS             complement(108..875)
                     /locus_tag="VEC56_03495"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HXV13249.1"
                     /translation="MKWTSQHVAFATALAVALSAAAVRSQDAAQLSETVKKTVETHQQ
                     TQKKQEEWAGEQAELTARLRAARAQVDFFEKKKALEEKEVAALDKGIAELERRMVESV
                     RLNESLIDTLNAVVTRLEAFVADDLPFLMEERRARIAGVKDALARPDYTGAEKLRRVL
                     EALQVEANYGSVAEVYQEKIIVNDEEVHADMVRVGRVSVYWLTPDGERVGEYDRAQGK
                     WVELDQKYIDTIIATREMALRMRSVEVIDLPLGRIQP"
     gene            1176..3923
                     /locus_tag="VEC56_03500"
     CDS             1176..3923
                     /locus_tag="VEC56_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV13250.1"
                     /translation="MRGTARRTGVISFLLAIAASTGAWAGTITGSVKDGKTGEPLIGA
                     TVGVVNTKLGGSTDLDGRYSIADVPAGLHSLRVMYAGYALKVVTGVAVAGEQTTSMDI
                     RLEPMTTEEGDAMRIEDIYVTAERLRTSATSILADRQRAAVIGDAISAEQIRLSPDGN
                     SSDALRRVTGLSIVDDKFVFVRGVTDRYNATTLNGVSVTGTDTDSDKKSFNFDLMPAS
                     LIASTVVLKTATPDLPGDFSGGLVQVNTLDLPNDFLAAANIEGGYDNISSRADIKAAP
                     GGGKDWTGQDDGSRALPSGLEGNALAQALPNTWGTAGDESRMNQTYGLAIGDRYQTGA
                     GEFGFIASGTYKNNFKVEQFHQEPYGEGIDGTRSNTPLFVFDGTRYKHRYLWGGLLNL
                     TYRPWGNHSFSAENNYTRSAEDKVVQSTGVVSDSTRYQTIEWDQRDLYLGQIAGDHVF
                     PQFNQLEFDWRASYSNSDAQEPDRKFAEYSRDPRGNYLLGENLRSWSTLEEDTRSAQA
                     DVTYPIGDTDIKAGYLYLKRERSYEIDAYTTDASKLSRPNRILIIEPIDEIFAAENYG
                     DGKFGFLPYSPLTGDYDGTQDLNTYYGMVDSPFRLAGQRFRFAGGARVEDSNQIVVSP
                     TSAQDPTPQTAQIDETDVLPSANLTYQVTGTSNVRLGYFESVNRPEFREMANVAYIDF
                     DANQGVIGNPDLKRALITNYDARLEWFPGPGEVLAVSYFYKEMTDAIEEQLLPSPDRY
                     VRTWFNSPEGKNYGFEVEIRTNLGFAWRQLENFVVQGNYTRVDSEVEYTESYTDPLGN
                     PITETKTRTMQGQAPYTVNAGLVYSIPDVGLSMSLLYNRFGRRLDAVGDTRDYDIFEE
                     SRDIVDFALTQQFYEWMRLKFTMKDLIGEDIVYTFGETGSVWESVKVGTTYAVSLSFS
                     L"
     gene            4167..>4467
                     /locus_tag="VEC56_03505"
     CDS             4167..>4467
                     /locus_tag="VEC56_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13251.1"
                     /translation="MKRLITMTAVAIAGYASAASAALPEVVIGAGTGAPVRTPYCQGF
                     DRTLSADTIYVLTGLYYVEPGASLTIEPGTIIKGHDATGGTLIVTRGGQIFAQGTP"
BASE COUNT          843 a         1467 c         1390 g          767 t
ORIGIN      
        1 ggccacgcgg tcggcttcgg ctttcctggc cgcttcgcga acgtcctgcg cggcggccac
       61 actcgaccac gctgccgtga gcgcgagcgc aatgatcgtc agtctcttca tggctggatc
      121 ctcccgagcg gcagatcgat gacttcgacc gatcgcatcc ggagcgccat ctcgcgcgtc
      181 gcgatgatcg tatcgatata cttctggtcc agctcgaccc atttgccctg ggcgcggtcg
      241 tactcgccca cgcgctcccc gtcgggggtg agccagtaca ccgagacgcg gcccacgcgc
      301 accatgtcgg cgtggacctc ttcgtcgttg acgatgatct tctcctggta gacctcggcg
      361 acgctcccgt agttggcctc cacctgcagc gcctccagaa cgcggcgcag cttctcggcc
      421 cccgtgtagt cgggtcgcgc gagcgcgtcc ttaacccccg cgatgcgcgc cctgcgctct
      481 tccatgagga agggcaggtc gtcggcgacg aaggcctcca gcctcgtcac caccgcgttg
      541 agcgtatcga tcaggctctc gttcaggcgc accgattcca ccatgcgccg ctcgagctcg
      601 gcgatgccct tgtcgagcgc cgccacttcc ttctcttcca gagccttctt cttctcgaag
      661 aagtcgacct gcgcgcgcgc ggcgcgtagg cgggcggtca actcggcctg ttcgccggcc
      721 cactcttcct gtttcttctg cgtctgctga tgcgtctcga cggtcttctt gaccgtctcg
      781 ctcagctggg ccgcatcctg cgatcgcacg gccgcagccg agagggccac ggccagcgcg
      841 gtcgcgaagg cgacgtgctg ggacgtccat ttcatgtggc ttcgagtcct ttcgtgccgt
      901 tgcggtgcgg tcgcggtaag ccgtgcggat tgcgcgagcg ttcatcgacc gaaccgaggt
      961 cgcaggcaga ttagccgggg cggataaatg cgagataaac gaaccgtgaa tcgaccgtga
     1021 atcccaagcg ccgtcgtatt ttcactgccc ctttaccgca ggttcacgcg cgactcctag
     1081 actaccggcg ttcggaaata cgacgtatcg acgccggtgt ctgggcgccc gcggcggtac
     1141 gagaagcgag gacgcgtgag gagggacacg aagacatgag aggaaccgcg agacgcaccg
     1201 gggttatctc gttcctgctc gccatcgccg catccacggg cgcctgggcg ggcacgatca
     1261 ccggttcggt caaggacggc aagaccggcg aaccgctcat cggggcgacg gtcggcgtgg
     1321 tgaataccaa gctgggcggg tccaccgacc tcgacggtcg ctactcgatc gccgacgttc
     1381 ccgcggggct gcactcgctg cgcgtgatgt acgcgggcta cgcgctcaag gtggtgacgg
     1441 gggtggcggt ggccggagaa caaaccacgt cgatggatat ccgactggag cccatgacga
     1501 ccgaggaagg cgacgcgatg cgtatcgaag acatctacgt gacggcggaa cgcctgcgca
     1561 cctccgcgac gtcgattctc gccgaccgcc agcgcgcggc cgtgatcggc gacgccatca
     1621 gcgcggagca gattcgcctt tcgcccgacg gtaactcgag cgacgcgctc cgccgcgtga
     1681 ccgggctgtc catcgtcgac gacaagttcg tgttcgtgcg cggcgtcacc gaccgctaca
     1741 acgcgaccac gctcaacggg gtctcggtga ccggtaccga caccgactcc gacaagaaga
     1801 gcttcaactt cgacctcatg ccggcatcgc tcatcgcgag cacggtggtg ctcaagaccg
     1861 cgacaccgga tctgccgggc gatttcagcg gcggcttggt gcaggtcaac accctggatc
     1921 ttcccaacga tttccttgcc gccgccaaca tcgagggcgg ctacgacaac atcagctcgc
     1981 gcgccgacat caaggccgcg ccgggcggcg gcaaggactg gaccggccag gacgacggga
     2041 gccgcgcgct tccctcgggc ctggaaggca acgcgctcgc gcaggcgctg cccaacacgt
     2101 ggggcaccgc gggcgacgag tcgcgcatga atcagaccta cgggctcgcg atcggcgacc
     2161 gctaccaaac cggcgcggga gaattcggct tcatcgcgtc ggggacgtac aagaacaact
     2221 tcaaggtcga acagttccac caggagccgt acggcgaagg catcgacgga acgcgcagca
     2281 acacgccgct gttcgtgttc gacggcacgc gctacaagca ccgatacctg tggggcgggc
     2341 tgctcaacct cacgtaccgt ccgtggggca accactcgtt cagcgccgag aacaactaca
     2401 cgcgctcggc cgaggacaag gtcgttcagt cgacgggagt ggtgagcgat tcgacgcggt
     2461 atcaaaccat cgaatgggat cagcgcgatc tctacctggg ccagatcgcc ggcgaccacg
     2521 tgtttccgca attcaaccag ctcgagttcg actggcgcgc gtcgtattcc aattcggatg
     2581 cgcaggagcc cgaccggaag ttcgcggagt actcgcgcga cccgcgcggt aactatctgc
     2641 tgggcgaaaa tctccgttcg tggtcgacgc tcgaggagga cacgcgcagc gcgcaggccg
     2701 acgtcaccta tccgatcggc gataccgata tcaaagccgg ctacctgtac ctgaagcgcg
     2761 agcggtccta cgagatcgac gcctacacga ccgacgccag caagctgagc cgccccaatc
     2821 gcattctcat catcgagccc atcgacgaga tcttcgcggc ggagaactac ggcgacggca
     2881 agttcggctt tctgccgtac tcgccgctga ccggagacta cgacggcacc caggacctga
     2941 acacctatta cgggatggtg gactcgccgt tccgactcgc gggccagcgc tttcgcttcg
     3001 ccggcggggc gcgcgtcgag gactcgaatc aaatcgtggt ctcgccgacg tcggcgcaag
     3061 accccacccc gcagacggcc cagatcgacg agaccgacgt gctgccgtcg gccaacctga
     3121 cctatcaggt cacgggtacg tcgaacgtgc gcctgggcta cttcgagtcg gtcaaccgcc
     3181 ccgagttccg cgagatggcc aacgtcgcgt acatcgactt cgacgccaac cagggtgtta
     3241 tcggcaaccc cgacttgaag cgcgcgctga ttaccaacta cgacgcgcgc ctcgagtggt
     3301 tcccgggtcc gggtgaagtg ctcgcggtca gctacttcta caaggagatg accgacgcca
     3361 tcgaagagca gttgttgccc tcgccggatc ggtacgttcg cacctggttc aactcgcccg
     3421 agggtaagaa ctacggcttc gaggtcgaga ttcgaacgaa cctgggcttc gcatggcggc
     3481 agctcgagaa cttcgtcgtt cagggcaact acacgcgcgt cgactcggag gtcgagtaca
     3541 cggagtcgta caccgatccc ttgggcaatc ccatcaccga gaccaagacg cgcacgatgc
     3601 aggggcaggc gccctatacc gtcaacgcgg ggctcgtcta ctcgatcccc gacgtgggtt
     3661 tgtcgatgag cctgctctat aaccgcttcg ggcggcgctt ggacgcggtg ggcgatacgc
     3721 gcgactacga catcttcgaa gaatcgcgcg acatcgtcga cttcgcgctg acgcagcagt
     3781 tttacgaatg gatgcgtctg aagttcacaa tgaaggatct tattggtgaa gacatcgtct
     3841 acaccttcgg tgagacgggc tcggtctggg agagtgtcaa ggtcggaacc acgtatgcag
     3901 tttctctctc tttcagtcta tagaggctga cgccttactc cgaccaaaat gattgagagg
     3961 gagggaacct gcacattcgg tcgcaaacgc aggaaagatt cggtggccat ccccggaggg
     4021 cgagctccgg ggatggcagg ccgagaagaa aggaggccga cggcggatca ggcacgcaac
     4081 ccccgttttt tctggcgtta ccagaataga ggaaatgtga agtcgatttc aaccagcaac
     4141 gaattctaaa taggaggaca gcatccatga agcgtctcat caccatgacg gcggtcgcca
     4201 tcgccggcta cgcgagcgcg gcgagcgccg ccctcccgga ggtcgtcatc ggcgccggaa
     4261 ccggcgcgcc ggtgcgaacg ccctactgcc agggcttcga ccgcacgctc tccgcggaca
     4321 cgatttacgt gctcacgggt ctctactacg tggaacccgg cgcctcgctg accatcgaac
     4381 ccggaacgat catcaagggc cacgacgcga ccggcggcac gctcatcgtg acccgcggcg
     4441 gacagatctt cgcgcagggc acgccgc
//
LOCUS       DASPGK010000122         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_968645, whole genome shotgun sequence.
ACCESSION   DASPGK010000122 DASPGK010000000
VERSION     DASPGK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968645"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VEC56_03510"
     CDS             <1..1407
                     /locus_tag="VEC56_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13252.1"
                     /translation="PIVFTSQYAPGARASGDWGGVIILGDAPTNKVNPLIEGGLIPGS
                     CSGGGGTYGGTDPSDDSGVFSYVRIEFPGFRFAANNEVNGLTMGGVGGQTQIDHVQVS
                     YSDDDSFEWFGGTVDCSYLVAFGGTDDEFDTDFGFRGDVQFGFGLRDPDQSDPTGESN
                     GFESDNDGSATSTATPYTRPIFSNITLVGPERTNAEVPFPLGETYQYSAVLRRSTQTN
                     IFNSVIMGYPWGLRIRDVNTIAWAGADSLQIRNTSVQATLQPSGSTHIHDETLWTGID
                     TWFTTGGWNNIGLATRLPSTILLNNLNDLNAPDPRPAAGSELIGSASFASPRLAGFVN
                     TTYRGAFPPVADAAPNELWTYYWTSFDPQTMDYSDGLPTAIGDAGPQYASHLSQNYPN
                     PFNPQTTIDFVVPATGPVTLEVFDARGAKVASLVDGVKSKGNHTVSFNAQGLASGVYF
                     YRLKGTGFNEMRKMVLLK"
     gene            complement(1486..2034)
                     /locus_tag="VEC56_03515"
     CDS             complement(1486..2034)
                     /locus_tag="VEC56_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13253.1"
                     /translation="MAALIATSTWTADARGQRSCADKRAESWEITIAGAKEKGDRLVV
                     EGIVTDAEGKPVAGAIVYAYQTDADGYYTREKDSNHVRLCGYMKTDADGRYRIHSILP
                     GSYPGSTIAAHIHYVVSDANVVEESFLLEFEGDRFVTQQSLERDRSRHQGLPESYRGI
                     RPLEKRDDGSLYCRRDLRVDTR"
     gene            complement(2045..2284)
                     /locus_tag="VEC56_03520"
     CDS             complement(2045..2284)
                     /locus_tag="VEC56_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXV13254.1"
                     /translation="MSLEEQLGSRFVCSKCQTHGATVKRISAAGTGLSKIFDIEHNQF
                     VTASCTNCGYTEIYNPEVLEGKRHLGSIFDILFGG"
     gene            complement(2339..3517)
                     /locus_tag="VEC56_03525"
     CDS             complement(2339..3517)
                     /locus_tag="VEC56_03525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV13255.1"
                     /translation="MNDRDVIVVGAGPAGSTAASVLARRGRDVLLVDRAEFPRDKVCG
                     DGIPPGTVGILNELGMAESLRAAGFQPIDSLRLVSARGRDWRLRFRPRHPGADFFIAP
                     RLRFDDLLRSHAVANGASFQTAHVRAPLVESGRVVGVRAESEGVERELRARIVIGADG
                     ATSAIARSLVPDKPPEAHRAVAIRAYLDDFDALPRTVEFHFGARFAPGYAWVFPLGER
                     RANVGVIVRTDRFKRRGLTIESLLREFIEGTELRARGARFDSLREVATWQLSLAAPDR
                     VPRAFDGALLAGDAARLVDALTGEGIHNAVVSGKLAGEAADRALASGHASRAALSADY
                     EARLEAELGRLVRRSYRYQKYITAYPPVLEAMFVLAGAFRVRVIRWLDRVSTDFRVAE
                     "
     gene            complement(3551..5293)
                     /locus_tag="VEC56_03530"
     CDS             complement(3551..5293)
                     /locus_tag="VEC56_03530"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXV13256.1"
                     /translation="MALLTGLVMMTSYAMAATPAESLKQLMAEHWEFEMREDPLFATQ
                     TGDHRFNDKLPSVAVADEVRREAATRDFLKRLEAIDRAQLGATDALNYDLLRRSFRNR
                     IGEYEFKTYLMPITNREGFHTDFPQLPDRVPLDNTKDYENYCARLEACNAYMNQYVGV
                     MREGVKAGYVLPKVVMEGWETAVDAHIVDDPTKSLLYAPFENYPDDVPAGDRAGLTER
                     GKKAITGSVVPGYRALRDFMAKEYVPACRDAIGASALPNGKAFYEHRVRYYTTLDLTP
                     KQVHDTGLSEVKRIRAEMDQVIAKAGFKGTFAEFLNFLRTDERFYVDTPEQLLKEVSF
                     ICKKMDGQLPKVFKTLPRMPYGVKPIPDFIAPKTTTAYYNQPAGDGSRAGTYYMNTYD
                     LKARPLYELEALSLHEAVPGHHLQIAIMQELPDVPNFRRYTYFTAFVEGWGLYSERLG
                     LETGFYEDPYSDFGRLTYEMWRAMRLVVDTGMHYFGWTRPQAIDYMAANGALSLHNVT
                     TEVDRYISWPGQAVAYKTGELKIRELRAWAERELGARFDVREFHDVVLWSGAVPLDVL
                     EANVRAWAVRVKKG"
     gene            complement(5417..>5899)
                     /locus_tag="VEC56_03535"
     CDS             complement(5417..>5899)
                     /locus_tag="VEC56_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13257.1"
                     /translation="GYLGLRFRPTAAWAVEAYADYEDRIDSGDRQTVHGFVGYKAEKA
                     RAGVQYIRQLRKETGEDVELQFVSAFLTGAITEKAWLFGRVDLNMDANPEGDRIAYIP
                     FDPTASNTFILVGLDYWVRETVGFSPNVEVVVYDEPDTAGLPTPDTDVIPRFTFHFTF
                     "
BASE COUNT         1002 a         1933 c         1927 g         1037 t
ORIGIN      
        1 cccatcgtgt tcacgagcca atacgcaccc ggcgcgcgcg catccggcga ctggggtggc
       61 gtgatcattc tgggcgacgc tccgaccaac aaggtcaacc cgctcatcga gggcggtctc
      121 attcccggtt cttgctccgg cggcggcggg acctacggcg gtaccgatcc gagcgacgac
      181 agcggcgtgt tcagctacgt gcgcatcgag ttccccggct tccgcttcgc ggccaacaac
      241 gaggtcaacg gcctcaccat gggcggggtc ggcggacaga cgcagatcga ccacgtgcag
      301 gtgagctatt cggacgacga ctcgttcgag tggttcggcg gcacggtgga ctgctcgtac
      361 ctggtcgctt tcggcggcac cgacgacgag ttcgacaccg acttcggttt ccgcggcgac
      421 gtgcagttcg gcttcggcct gcgcgacccc gatcagtcgg acccgaccgg cgagtcgaac
      481 ggcttcgagt cggacaacga cggctcggcc acttccaccg cgacgccgta cacgcgtccg
      541 attttcagta acatcacgct ggtcggcccc gagcgcacca acgccgaagt accgttcccg
      601 ctcggggaga cctaccagta cagcgccgtg ctccgccgct cgacgcagac caacatcttc
      661 aactcggtga tcatgggcta cccgtggggg ctgcgcatcc gcgacgtcaa cacgatcgcg
      721 tgggcgggcg cggactcgct gcagattcgc aataccagtg tgcaggccac gctgcagccc
      781 agcggctcga cgcacatcca cgacgaaacg ttgtggaccg gtatcgacac gtggtttacc
      841 accgggggct ggaacaacat cggtctggcc acacgcctgc cgagcacgat tctgctcaac
      901 aacctgaatg atctgaacgc acccgatccg cgtcccgcgg cgggctccga gctcatcggt
      961 agcgcgagct tcgcaagccc gcgtctggcg ggcttcgtga atacgaccta ccgcggcgcg
     1021 ttcccgccgg tggccgacgc ggcccccaac gagttgtgga cctactactg gaccagtttc
     1081 gacccgcaga cgatggacta cagcgacggc ctcccgacgg ctattggcga cgccggtccg
     1141 cagtatgcgt cgcatctctc gcagaactat ccgaacccgt tcaacccgca gacgacgatt
     1201 gacttcgtcg tccccgcgac gggcccggta acgctcgagg tgttcgacgc gcgcggcgcc
     1261 aaggtggcct cgctggtcga cggtgtgaag tcgaagggca accacacggt gagcttcaat
     1321 gcccagggcc tcgcttcggg cgtgtacttc taccgcctca agggcacggg cttcaacgag
     1381 atgcggaaga tggtcctcct gaagtagtgt tttcaggagt gaatcgcttt cgcgtgagtg
     1441 gacgcgcccc ggcgagcaat cgtcggggcg cgcttcgttt ccgggctagc gagtgtcgac
     1501 gcggaggtcg cggcggcagt agagcgaccc gtcgtcgcgc ttctcgagcg ggcggatgcc
     1561 gcgatagctc tccgggagtc cttggtggcg ggaccgatcg cgttcgagtg actgctgggt
     1621 cacgaagcga tcgccctcga actcgagcag gaagctctcc tcgacgacgt tcgcgtccga
     1681 cacgacgtag tggatgtggg cagcgatggt gctgccggga tagctgcccg gcaggatgga
     1741 atgaatgcga taacgcccgt cggcgtcggt cttcatatat ccgcacaagc gcacgtggtt
     1801 ggagtccttc tcgcgggtgt aatagccgtc ggcgtcggtc tggtacgcgt atacgatggc
     1861 gcccgcgacc ggcttgcctt ccgcgtcggt gacgatgcct tccacgacca gccggtcacc
     1921 cttctccttc gcacccgcga tcgtgatttc ccaagactcc gcacgcttgt cggcgcagga
     1981 acgctgccca cgggcatccg ccgtccaggt cgaggtggcg atcaatgccg ccacgactgc
     2041 ggcgctaccc gccgaacaga atgtcgaaga tggagccaag gtgtcgcttc ccttccagca
     2101 cttcggggtt gtagatctcg gtgtacccgc agttcgtgca cgacgccgtg acgaactgat
     2161 tgtgctcgat gtcgaatatc ttcgaaagcc cggtgccggc cgccgatata cgtttgacgg
     2221 tggcgccgtg ggtctgacac ttactgcata cgaatcgtga gccaagttgt tcctcgagtg
     2281 acatattgcc tccttgtgga ctccagtaac cgagattccc gacggtattc gaaagcgcct
     2341 attccgccac ccggaagtcg gtggaaacgc ggtccagcca ccggatcact cgcactcgga
     2401 aggcgccggc caatacgaac atggcctcga gcaccggggg gtaggccgtg atgtattttt
     2461 ggtaccgata cgagcgacgt accaagcgac ccagctcggc ttcgagccgc gcctcgtagt
     2521 ccgccgacag cgccgcccgg gacgcgtggc cggatgccag cgcgcgatcg gccgcctcgc
     2581 ccgcgagctt gccggacaca accgcgttgt gaatgccctc tccggtcagc gcgtcgacga
     2641 gccgggccgc gtcgccggcc aacaatgcgc cgtcgaacgc gcgcggcacg cggtccggag
     2701 cggccagcga aagctgccag gtcgcgacct cccgcaacga atcgaagcgg gccccgcgcg
     2761 cgcgcagttc cgtcccctcg atgaattcgc gcaagagcga ctcgatggtg agaccgcgcc
     2821 gcttgaaacg gtccgtgcgc acgatcacgc ccacgttggc gcgccgttct ccgaggggga
     2881 acacccacgc gtagcctggc gcgaagcgcg ctccgaaatg gaactcgacc gtccgcggca
     2941 gcgcgtcgaa gtcgtcgagg taggcgcgga tcgccaccgc gcggtgcgcc tcgggaggct
     3001 tgtcggggac gagagagcgc gcgatcgcgg acgtcgcgcc gtcggcgccg atcacgattc
     3061 tcgcccggag ctcgcgctcg acgccctccg actcggcgcg cacaccgacg actcgtccgg
     3121 attcgaccag cggagcacgc acgtgcgcgg tctggaagga tgcgccgttc gccacggcgt
     3181 gcgagcgcag caaatcgtcg aagcggagtc gcggcgcgat gaagaaatcg gccccggggt
     3241 gtcgcggccg gaagcgcagg cgccagtcgc gtccgcgcgc cgaaaccagt cgcagcgagt
     3301 cgatgggttg gaatcccgcg gcgcgaagcg actcggccat gccgagctcg ttcaagatac
     3361 cgaccgtgcc cggcgggatc ccgtcgccgc acaccttgtc gcgcggaaac tccgcgcgat
     3421 ccacgagcag cacgtcgcga ccgcgccgcg cgagtacgct cgccgccgtg ctgcccgcgg
     3481 gacctgcgcc gaccacgatg acgtcgcgat cgttcatggg ccgttgcggc cggtccgcgc
     3541 gacgaaccgg ctatcctttc ttgacccgca ccgcccaagc gcgcacgttg gcctcgagga
     3601 cgtccagcgg caccgcaccg ctccacagga cgacatcgtg gaactcgcgc acgtcgaacc
     3661 tcgctcccag ctccctctcc gcccacgcgc gcagctcgcg gatcttgagc tcgccggtct
     3721 tgtacgccac cgcctggccg ggccacgaga tgtaacggtc gacttcggtg gtcacgttgt
     3781 gcagcgagag cgcgccgttg gccgccatgt agtcgatggc ttgcggtcgc gtccacccga
     3841 agtagtgcat gccggtgtcg acgacgagac gcatcgcgcg ccacatctcg taggtcaaac
     3901 ggccgaagtc gctgtaggga tcctcgtaga accccgtctc gaggccgagg cgctcggagt
     3961 aaaggcccca cccttcgacg aaggccgtga agtaggtgta gcgccggaag ttggggacat
     4021 cggggagctc ctgcatgatt gcaatctgga ggtggtgacc cggcaccgct tcgtgcagcg
     4081 aaagcgcttc cagctcgtag agcggccgtg ctttcaagtc gtacgtgttc atgtagtagg
     4141 ttcccgcgcg cgatccgtct cccgccggct ggttgtagta ggccgtcgtc gtcttgggcg
     4201 cgatgaagtc ggggatgggc ttgacgccgt agggcatgcg cggaagcgtc ttgaagacct
     4261 tgggaagctg gccgtccatc ttcttgcaga tgaacgagac ttccttgaga agttgttcgg
     4321 gagtatcgac gtagaagcgc tcgtcggtgc ggagaaagtt gaggaactcc gcgaacgtcc
     4381 ccttgaaccc cgccttcgcg atcacttggt ccatctcggc gcggatgcgc ttgacctccg
     4441 agagcccggt gtcgtgcacc tgcttggggg tgagatcgag cgtggtgtag tagcgaacgc
     4501 ggtgttcgta gaacgccttc ccgttgggaa gcgcggaggc gccgatggcg tcgcggcacg
     4561 cgggcacgta ttccttggcc atgaagtcgc gcagcgcgcg ataacccggg accaccgagc
     4621 ccgtgatcgc cttcttaccc cgttcggtca agcccgcgcg atcgcccgcg ggaacgtcgt
     4681 cggggtagtt ctcgaagggc gcgtacaaca agctcttggt tggatcgtcg acgatgtggg
     4741 cgtccaccgc ggtctcccac ccctccatga ccaccttcgg cagcacgtac ccggccttga
     4801 caccctcgcg catcacgccc acgtactgat tcatgtaggc gttgcacgcc tccaggcgcg
     4861 cgcagtagtt ctcgtagtcc ttggtgttgt cgagcggcac gcgatcgggc agctgcggaa
     4921 agtcggtatg gaagccctcg cggttggtga tgggcatgag gtaggtcttg aactcgtact
     4981 cgccgatccg gtttcgaaac gaacgacgca gcaagtcgta gttgagcgcg tccgtggccc
     5041 cgagctgggc gcggtcgatc gcctccagcc gcttcaagaa gtcccgcgtg gccgcttcgc
     5101 ggcgcacttc gtcggccacc gcgaccgagg gcagcttgtc gttgaagcga tgatcgccgg
     5161 tctgggtcgc gaagagcggg tcctcgcgca tctcgaactc ccagtgctcg gccatcagct
     5221 gcttcaagct ttcggccggc gtggcggcca tcgcgtagga cgtcatcatc accaatccag
     5281 tcaatagggc catcgttctc accgcgatct cctttgggtt gtcgggaaga acttggagac
     5341 tacacaaagc gggatacggc ggcaaccgcg ccatgttgcg cgcggttgct cgtcggatcc
     5401 cgcggcgggc acctggctag aacgtgaaat ggaaggtgaa gcgggggatg acgtcggtat
     5461 ccggcgtcgg cagcccggcc gtgtccggct cgtcgtagac cacgacttcg acgttggggc
     5521 tgaagcccac cgtctcgcgc acccagtagt cgaggccgac caggatgaac gtattggagg
     5581 cggttgggtc gaagggaatg tacgcgatcc ggtcgccctc cgggttggcg tccatattga
     5641 gatcgacgcg accgaagagc caggcttttt cggtgatggc accggtgagg aacgcgctca
     5701 cgaactgcaa ttcgacgtct tcgcccgtct ccttgcggag ctgccggatg tactgaacgc
     5761 ccgcgcgggc tttctccgct ttgtatccca cgaagccgtg caccgtctgg cggtcgcccg
     5821 agtcgatgcg gtcttcgtag tccgcgtacg cttccacggc ccacgcggcc gtggggcgga
     5881 agcgaagccc caggtaacc
//
LOCUS       DASPGK010000123         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_852678, whole genome shotgun sequence.
ACCESSION   DASPGK010000123 DASPGK010000000
VERSION     DASPGK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852678"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VEC56_03540"
     CDS             complement(<1..1138)
                     /locus_tag="VEC56_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13258.1"
                     /translation="MQFHPLLAGLVVIALHAPAAAQPAPGTTVYDLSTWAEAADTLRG
                     VTAGARVTLGAAFLEAGSVRVEINGVETSRDAYQVNYHLGTIRFTDEVPAGATVIVRY
                     RRRPFLIAPVYTLRPADVSPTEPAEQAPAREVAVRPREPSTQPDLVFGGTKSVSFSTG
                     SNRGSTLDQSLEATVEGKLTPTISVRALLSDNNLPIQPEGNTEELEYFDRVFVEIEGP
                     RARAAVGDISLDNRTSTFSPLVRQLRGFSGGAWSSRGRVTAAGAETKGEFRSVTFRGT
                     TGLQGPYALLSPARTTLDVIIAGTERVSIDGLRVERGPNRDYLIDYDAGTITFTPRRL
                     ITTDTEIAVDFEATAENYSRSTLLGGAERVTLGGGVAMSVLYARE"
     gene            complement(1155..2039)
                     /locus_tag="VEC56_03545"
     CDS             complement(1155..2039)
                     /locus_tag="VEC56_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HXV13259.1"
                     /translation="MESVAPVWAMRACDRVTFGAPAALCVDFGGTVYLADASPPRLLS
                     YTESSQRCVEFQTPDEWPAFRPSDVAVRGFFVYAIDEPDRALLRWDASGSWRDVLLNF
                     EDITTPGRRISPYGLDVHASTGRVAVTDVENHRVILLDTYLNVDVSFGNYGAFEGQLD
                     TPLGISFTPRGELLVADSGNARVQFFSDAGTFRRAIPADGAASPMRRPRRAVATEDGR
                     VLVADPGAGRVFEFAPDGVLARALTPEGSARFEPSDVAVGRGGRVYVTDAASQTLFAF
                     DAAPPARADSPTSTTKEP"
     gene            complement(2228..>2745)
                     /locus_tag="VEC56_03550"
     CDS             complement(2228..>2745)
                     /locus_tag="VEC56_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13260.1"
                     /translation="ATFFAVEGVIWASFIAFQVQGGSLEEEYQDLAVIFAGVTRTDHS
                     DEFYATIRDYDSAQEYEASIKNEGQFELYPNIGSEALDQYFTDHRVEDFEPWLWASTD
                     RRLQYSEVRSASKTAYRRADYMLAAAAANRVVSAIFAYAAARSVAKHEVGLNVDVTPR
                     GEVALTLTKGF"
BASE COUNT          436 a          904 c          997 g          408 t
ORIGIN      
        1 attcgcgcgc gtacaagacg ctcatcgcga ccccgccccc cagcgtcacg cgctccgcgc
       61 cgccgaggag tgtcgagcgc gagtagtttt ccgccgtcgc ctcgaagtcg accgcgatct
      121 cggtgtcggt cgtgatcagg cgccgcggcg tgaaggtgat ggttccggcg tcgtagtcga
      181 tcaggtagtc gcggttgggc ccgcgctcga cgcgcagccc gtcgatggag acgcgctcgg
      241 tgcccgcgat gatcacgtcg agggtggtgc gcgccggtga gagcagcgcg tacggtccct
      301 gcaggccggt ggtgccgcgg aaggtcacac ttcggaactc ccccttggtc tccgcgccgg
      361 ccgcggtcac gcgtccccgc gacgaccacg cgccacccga gaacccgcgc agctggcgca
      421 ccaagggaga gaacgtcgag gtccgattgt cgagcgagat gtcgcccacc gcggcgcgcg
      481 cgcgcggccc ttcgatctcc acgaacacgc ggtcgaagta ctcgagctcc tcggtgttgc
      541 cttcgggctg gatgggcagg ttgttgtccg agagcagcgc gcgcaccgag atggtgggcg
      601 tgagcttccc ttcgaccgtc gcctccagcg actggtcgag ggtcgagccg cggttgctgc
      661 cggtcgagaa ggacaccgac ttggtaccgc cgaataccag gtcgggctgg gtcgacggct
      721 ctcgcggtcg caccgcgacc tcgcgcgccg gggcctgctc ggcgggttcg gtgggcgaca
      781 cgtccgcggg ccggagcgtg tacacgggcg cgatgagaaa gggccggcgc cgataccgaa
      841 cgatcacggt ggcgccggcg ggaacctcgt cggtgaatcg aatcgtgccg aggtgatagt
      901 tgacttggta ggcatcgcgc gacgtctcga cgccgttgat ctcgacgcgc acactacccg
      961 cttccagaaa cgcggcaccc agcgtgacgc gcgcgcccgc ggtcacgccg cgcagcgtgt
     1021 cggccgcttc ggcccaggtg gagagatcgt agacggtggt accgggggcg ggttgtgcgg
     1081 ccgccggcgc gtggagagcg atgacgacca agccggcgag cagggggtgg aactgcacgc
     1141 ggaatcggtc gatgtcacgg ctccttggta gtcgaggtgg gcgagtctgc gcgggccggg
     1201 ggtgccgcat cgaacgcgaa cagcgtttgc gacgcggcgt cggtaacgta gacgcgtccg
     1261 ccgcgtccca ccgcgacgtc gctgggctcg aagcgcgcgc ttccctccgg cgtcagcgcg
     1321 cgcgccagca caccgtcggg ggcgaattcg aacacgcgcc ccgcgcccgg gtcggccacc
     1381 agcacgcggc cgtcttcggt cgccaccgcg cggcgcgggc gccgcatcgg cgaggccgcc
     1441 ccgtcggccg gtatcgcgcg gcggaaggtc cccgcgtcgg agaagaattg cacgcgcgcg
     1501 ttgccgctgt cggccacgag caattccccc cggggcgtga acgagattcc gagcggcgtg
     1561 tcgagctggc cctcgaaagc accgtagttg ccgaacgaca cgtccacgtt gagataggtg
     1621 tcgagcagga tcacgcggtg attctcgacg tcggtcaccg ccacgcggcc ggtcgaagcg
     1681 tggacgtcga gcccgtaggg cgagatgcga cgccccggtg tggtgatgtc ttcgaagttg
     1741 agcagcacgt cgcgccagga accactcgcg tcccagcgca agagcgcgcg gtcgggctcg
     1801 tcgatggcgt agacgaagaa tccgcgcacg gccacgtccg aggggcgaaa ggccggccac
     1861 tcgtccgggg tctggaactc cacgcagcgc tgcgacgact cggtgtacga caagaggcgc
     1921 ggaggcgacg cgtccgccag atacacggtc ccgccgaagt ctacgcacag cgccgcgggc
     1981 gcgccgaagg tcacgcgatc gcacgcgcgc atggcccaca cgggagcgac ggattccacg
     2041 ggggaaggcg ccggaaccgc gggagcgtcg gtgcgagtct cgttggagga cggcgcggcg
     2101 gacgcgcacg cgagggacag ggcgaggaga atgatcaaga tactgcgcat actgctacgt
     2161 gcattcgcgg ccgtctcgcg ctgccgttgg agcggagagg ggcgatcgta ccggccggcc
     2221 tgggaaatca gaaccccttc gtcaacgtga gggcgacttc gccgcgcggg gtcacgtcga
     2281 cgttcaatcc cacctcgtgc ttggcgacgc tgcgcgccgc ggcgtacgcg aagatcgcgc
     2341 tcacgacacg gttggcggcc gccgccgcga gcatatagtc ggcccgccgg tacgcggtct
     2401 tgctggccga gcgcacctcg gagtactgca gacgccggtc ggtcgaggcc cacagccacg
     2461 gctcgaagtc ttcgacgcgg tggtcggtga agtactggtc gagcgcctcg cttccgatgt
     2521 tgggatacaa ctcgaactga ccctcgttct tgatgctcgc ttcgtactcc tgggccgaat
     2581 cgtagtcgcg aatggtggcg tagaactcgt cggagtggtc cgtgcgggtg acgccggcga
     2641 aaatcaccgc caggtcctgg tactcctctt cgaggctgcc gccttgcacc tggaacgcga
     2701 tgaacgaagc ccagatgacg ccttcgaccg cgaagaacgt cgcgg
//
LOCUS       DASPGK010000124        11115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1672351, whole genome shotgun sequence.
ACCESSION   DASPGK010000124 DASPGK010000000
VERSION     DASPGK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672351"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VEC56_03555"
     CDS             complement(<1..357)
                     /locus_tag="VEC56_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV13261.1"
                     /translation="MTLVGSKILHYRILQEIGAGGMGIVYLAEDEKLHRRVALKFLAK
                     SIQDDPDARDRLMREARAASALNHPNIVAIHAIESTPDQVFIVMEYVEGESIHGLVKS
                     GRMTWQRAVELAPQILS"
     gene            complement(420..1061)
                     /locus_tag="VEC56_03560"
     CDS             complement(420..1061)
                     /locus_tag="VEC56_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXV13262.1"
                     /translation="MYNPRSYRNESISELHEFVRRYNFAALFTHNGGESFATHLPFMI
                     DPERGRLGTLVAHMARANPHWRAFEGAPPSLVVFSGPNAYISPAWYQEQVTVPTWNYT
                     VVHASGPARLIDDPALLRAMVLRLVNLHEAPLGHPWDLRKAEAVMDVELEGIVGFEIP
                     VERLEGKFKLNQNRSVEDREGVVRALEGSSHPDEREIARLMRAQLDSDRNSKR"
     gene            complement(1174..1605)
                     /locus_tag="VEC56_03565"
     CDS             complement(1174..1605)
                     /locus_tag="VEC56_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXV13263.1"
                     /translation="MRTRGFTLIELMIVVVIIGILAAIAVPNFVRMKANAQDAGVKSN
                     SHAVQVAAEDFAVKNDGFYAADLTDLCTTGETVIDALPQNQRLENPFTRALTEPVDGP
                     AAAIGQTGYEPVVDGSGMTIGYIITGQGNGGQQICRFTNGM"
     gene            complement(1802..2482)
                     /locus_tag="VEC56_03570"
     CDS             complement(1802..2482)
                     /locus_tag="VEC56_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13264.1"
                     /translation="MAKKPEESKPAPVAESNVIRFPVEAVSRFGFKRAHVKRGRVGRM
                     EEEGQMNLFDPSARRGGPRMGDVVPLPMQMGAFEAALLLDDANDPRAEAMYQRAIEEG
                     DYTADAWCNLGVLKSVRRNLDDAFQCFAQSLALDPRHAESHYNIGNLYFEYEDLKLSR
                     MHYEKAIECDPSLASAYFNLGLVTALTEDYQVAYDSLLHYKTLAPGEDSSVADELMRY
                     LQTAIRLS"
     gene            complement(2488..3390)
                     /locus_tag="VEC56_03575"
     CDS             complement(2488..3390)
                     /locus_tag="VEC56_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXV13265.1"
                     /translation="MAALRSIWKGHLRFSLVTIPIRVYNAVDTAESISFNQIHRECNG
                     RIGYDKRCKKCNNLVENDEILKGFQYEPDQYVVFEKDDLDKLKLKSTKVIDIEGFVDA
                     SEIDATLYDSPYYAGPDGDVAAKTYGLLSAALRDSGKLGVGKVVLRDREDVVMIAPKG
                     DGIVLYKMRYPKEIRKIEDVPLVEKHKANKDELKLAKSLIDSMSTTFGAMKLENKYND
                     AVREMVQAKIAGKEIVAIEEVEPEVVDIMTALKQSIEQAKGKKKPMEKVTAEKAVAKE
                     KKSAKSKATTTAKSKAKTKARKTA"
     gene            complement(3537..4541)
                     /locus_tag="VEC56_03580"
     CDS             complement(3537..4541)
                     /locus_tag="VEC56_03580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV13266.1"
                     /translation="MMLRSVVAMVLCSFLPAPAMAADAPFDSSAALARARELRPFVSS
                     GTPGALWTAFAPPMREAMGDSLRFAAIMASIHGQVGAITEVIDERLLREMGWTYRARC
                     RFEKAPMPLVLVLSFDDDARVSGLLVQPEKQPFASTKLDYVAKTTLELPFKGEWYVFW
                     GGRTIDENYHAISKSQRFAYDLLIMKGGVSHAGDGKALADYSSYGAEIFAPAAGTVVW
                     SCDSLPDQQPGQMDPKNPIGNGVVIDHGNGEFSLLAHMQPKSLKVKTGDPVKAGAPLG
                     LCGNSGNTSEPHIHYHLQDGPDLKTAEGLPAPFTDLIVNGKPVVKAELTKGQSVRRAR
                     "
     gene            complement(4538..4957)
                     /locus_tag="VEC56_03585"
     CDS             complement(4538..4957)
                     /locus_tag="VEC56_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13267.1"
                     /translation="MIAGCRANGSEREGVAVTCGTVLGLDAENAARKDWSGETREWSA
                     EAIGDTLRLVRKGRCGDECTFVDEIVLAGLSARCPSLVRATTTRRDAGSPVPGAKIVE
                     AQHGSLAIQDWDARGVVSGRLVAEISLMFYVTLAKEE"
     gene            5107..7608
                     /locus_tag="VEC56_03590"
     CDS             5107..7608
                     /locus_tag="VEC56_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13268.1"
                     /translation="MKRLFLVVAVLAISASIPRTSIVDASADEPLWRCGTELTQSDID
                     QFKAREQRPRNGFIPQGAPAPPYCIPIAAHIVRTSGGTGGLSLDRLDQGIVDCNTMYQ
                     NTGMVFQLLSVDYIDDDDYYYNVNTDAEIDAMIQENVVADAINVYFTPNLSNEDGGLC
                     GRGSFTTSTPQGVAMNNGCTGTSDNPSTFPHELGHFFDLFHTHETAFGDELVDGSNCG
                     SAGDKLCDTPADPGLDEGTNVNSYPSCSYYGGATDGNGDSYNPDTHQLMSYAPKECRD
                     VMSPQSETKVVATLLNERPELLNKGCAPTANAGGDIIAECTGPSLTQVQLDGTGSSDP
                     EGGALTYSWSATGIAFDDDTSSQPTGGFPFGTTTVTLTVSDGNYTRTDEVNVTVDDTT
                     PPDITCPSDITVECESHCGTPATNAAIAAFLADVSATDVCDASLTIENNAPTCFPEGA
                     TVVTFTTEDSHGNDISCQATVTVEDTTPPEITVVLDRDALWPPNHKMVEVCVDFIEVT
                     DICDDAPTYVLQEITSDEPENDVGDGNTEPDIQGADFETPDLCFDLRAERQGTENGRT
                     YTIVYRAEDGAGNEAFATVYVEVPHDQSANAMAASGYSPEGTEFIGDRFAVVIPSTDG
                     IDAAAIDPNRVYVGNTRGATRPVASRLVDVNADGREDLALEFPSNEAMSYMPVLMNVD
                     PEAGGLQSIYDGPVGVHFNAGGIDYLVSDIFALGEPVEIPTLGAVAPPLVVTNDVVST
                     PRETGFKAIHPNPFNPQTTVTFTLASPEHVRIAIYDVRGTLVRRLEDRAMPAGEHSVV
                     WDGRDDGGRTGSSGIYFVRMLAGRTVETRKIVMLK"
     gene            complement(7669..8370)
                     /locus_tag="VEC56_03595"
     CDS             complement(7669..8370)
                     /locus_tag="VEC56_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV13269.1"
                     /translation="MTGRCNVCGWSDRFLRLDRGREGTVCRNCGSTSRDRAVAHAFAR
                     VLDEDQAVFRWTYRLKTRLLDSGTRGSLAMYFRMKFDCRGTELPYDDETFDFVVAADV
                     FDRAGRDIEAIGEVQRVLKSGGYLVLTAPHDGDLIDVMRAPDFYAERIAVDVPEFGIA
                     PQSVFVGRKDRPVALSPARALRAGSTGPMIPFRLFVALKYGKHPFSPVRDFCPYNFVR
                     GFPHLFRDARHARSR"
     gene            complement(8385..10706)
                     /gene="ligD"
                     /locus_tag="VEC56_03600"
     CDS             complement(8385..10706)
                     /gene="ligD"
                     /locus_tag="VEC56_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXV13270.1"
                     /translation="MPRSSQVVQVGKRTLELSNLNKVLWPEDGVLKAELIQYYLAIAP
                     TILTHIRGRPLSLVRFPDGIHGETFFQKNLPGWVPDWLESVEIGDAKKTINYVIATEE
                     ASLVWLANMACIELHQMHCRAKNRDHPDYFVFDVDPPDGTPWAQIVEVGEGLRAAFEQ
                     HGYHAFVKTTGRKGLHVVAPIEPKWDFDTVFKACESIAKPFVAANSKTCTLRIHKEQR
                     DNKILIDIYRNRPSQTIVAAYSVRGLPGATVSMPLKWEQLHTMDGIRDFHLRNVPDIV
                     KRDGDPWEAMEAYAVELHTHRSAPKPTKKLEKSGKRKTPEQLKSYEKKREFSKTPEPA
                     PQPLEEGTNRFVVHRHHASRLHYDLRLEKDGVLKSWAVPKGLPPRPGIKRLAVQTEDH
                     PIEYLNFEGTIPKGEYGGGDMWVFASGKYEVTKAKKDSTYVRLSGRGLTGEYRIIPTR
                     GNECLLERVDAPQTDWLVEPVEFMLADLSDKVPSVGDWIYEVKWDGIRAMIALDEGVV
                     RIRSRNQNDITVAFPELADPGSFRATCGLFDCEIVCLDSAGRPLFKDVINRMRQTSEG
                     AVARAKSKHPAVCYVFDCLYLDGRPLMNEPLTRRREWLADAIKKESAYRVSQTVEDGD
                     ALFEAAREAGLEGIMAKKATSPYTPGRRNDAWLKVKTRRTMDCLVVGYTQGKGDRAKT
                     FGALHIAERRGDAWAYLGKVGTGFDDRTLEAVAAEVRALREAKRPFKEKPIDDAISVW
                     VEPTLWCEVQYASLTPNGTLREPVFVRLRPDRA"
     gene            10735..>11115
                     /locus_tag="VEC56_03605"
     CDS             10735..>11115
                     /locus_tag="VEC56_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXV13271.1"
                     /translation="MNLYAGTSGFSYKEWKGHFYPEKIAPDQMLNFYSTKLPAVEINN
                     TFYRLPRREALEAWAAQVPPDFRFAVKASQKITHMKRLKNADDETGYLMDVVGAMEKK
                     LGVVFFQLPPNFKKDVERLGAFLGT"
BASE COUNT         1890 a         3563 c         3635 g         2027 t
ORIGIN      
        1 ggagaggatc tgcggcgcca gctcgaccgc gcgctgccac gtcatcctgc cggacttcac
       61 caagccgtga atcgactcgc cctcgacgta ttccatcacg atgaacacct ggtcgggtgt
      121 ggactcgatg gcgtggatcg caacgatatt gggatgattg agcgcggagg cggcgcgcgc
      181 ctcccgcatc aagcgatcgc gggcgtccgg gtcgtcctgg atggatttgg ccaggaactt
      241 gagcgcgacg cggcggtgga gtttctcgtc ctccgccagg tagacgatgc ccatgccgcc
      301 ggcgccgatc tcctgcagga tgcgatagtg aaggattttg ctgccgacga gcgtcatgat
      361 ccggacagtt taggcgacac ggatggattc cgcaaccggg atgccggtaa tacgcggcgc
      421 tagcgcttcg agttgcggtc cgagtccagt tgcgcgcgca tcagacgggc gatctcgcgc
      481 tcgtcggggt gggacgaccc ttcgagcgcg cgtaccacgc cctcgcgatc ctcgaccgag
      541 cggttctggt tgagcttgaa cttgccctcc agacgttcga cggggatctc gaaaccgacg
      601 attccctcga gctcgacgtc catgaccgct tccgccttgc gcaggtccca tgggtggccg
      661 agcggcgcct cgtgcagatt caccagccgc agcaccatcg cgcgcagcag cgccgggtcg
      721 tcgatcaagc gcgcggggcc ggacgcgtgc accacggtgt agttccaggt cggcacggtt
      781 acctgctctt ggtaccacgc gggcgagatg tacgcgttcg gccccgagaa caccactagg
      841 gaaggaggtg cgccttcgaa cgcgcgccag tgcgggttgg cgcgcgccat gtgcgcgacc
      901 agcgtcccca gcctcccgcg ctcggggtcg atcatgaacg gcaagtgggt cgcgaacgac
      961 tcgccgccgt tgtgcgtgaa caacgcggcg aagttatagc ggcgaacgaa ctcgtggagc
     1021 tccgagatgc tttcgttacg gtaggagcgc ggattgtaca cgcctggaag gatacgaaag
     1081 ccggtcgcag cgggccaggg aagaaatggg ggccggaccc gatctggggg cccgaccccg
     1141 tctcattttg gtttcgagtt gaattcgttg gagctacatg ccgttcgtga agcggcagat
     1201 ctgttggccg ccgttgccct ggcccgtgat gatgtagccg atggtcatac cactgccgtc
     1261 caccaccggc tcgtagccgg tctggccgat tgcggcggcg gggccgtcga cgggctccgt
     1321 gagggcgcgg gtaaagggat tctcgaggcg ctgattctgg ggaagcgcgt cgatgaccgt
     1381 ctcgcccgtg gtgcacaagt cggtgagatc ggcggcatag aaaccgtcgt tcttcacggc
     1441 gaagtcttcc gccgcgacct gcacggcgtg gctgttcgac ttgacgcccg cgtcctgggc
     1501 gttggccttc atgcggacga agttgggtac tgcgatggcg gcgagaatac cgatgatcac
     1561 aacgacgatc atcaattcga tgagtgtgaa tcctctggtt ctcatagcgg tcctcactgt
     1621 gtcattcgcg cggcgtttgc ggcgcacgcg cgggccgatt tagctccgac ccgggtaacg
     1681 caagccccgt gccacagaac ggagaacggt cgccggcgct tcggggcgcg tcgcgcgagg
     1741 gtgtggaagg gagcgggatt tctcggtgcc gggacggggc gaaacctgga acggtcgaat
     1801 cctaggagag gcgaatcgcg gtttgaaggt agcgcatcag ctcgtcggcc accgaggaat
     1861 cctcgcccgg agcgagcgtc ttgtaatgga ggagtgagtc gtacgccact tggtagtcct
     1921 cggtgagcgc ggtcacgaga cccaaattga aataagcgct cgccagggac gggtcgcact
     1981 cgatcgcctt ctcgtagtgc atgcgcgaga gcttcaagtc ctcgtactcg aagtagagat
     2041 tcccgatgtt gtagtgcgac tccgcgtgac gcgggtcgag ggcgagcgat tgcgcgaagc
     2101 attggaaggc gtcgtcgagg ttgcggcgaa ctgacttcaa gacacccaag ttgcaccacg
     2161 cgtcggcggt gtagtcgcct tcttcgatgg cgcgctggta catcgcctcc gcgcgcggat
     2221 cgttggcgtc gtccaagagg agggcggcct cgaaggcccc catctgcatg ggcaggggga
     2281 ccacgtcgcc catgcgcggc ccgccgcggc gcgcggacgg atcgaagagg ttcatctggc
     2341 cctcttcttc catgcggccc acgcgaccgc gcttcacgtg cgcgcgcttg aagccgaaac
     2401 gcgataccgc ctccaccggg aagcggatca cgttgctctc ggcgaccggg gcgggcttgg
     2461 attcttcggg tttcttcgcc actcgtgcta cgccgtcttg cgcgccttgg tcttcgcctt
     2521 ggatttcgcc gtggtcgtag ccttggactt ggccgacttt ttttccttgg ccaccgcctt
     2581 ctccgcggtc accttttcca tcggcttctt cttgcccttg gcctgctcga tgctctgctt
     2641 caaggccgtc atgatgtcga ccacttccgg ttcgacctct tcgatcgcga cgatctcctt
     2701 gcccgcgatc ttggcctgta ccatctcgcg cacggcgtcg ttgtacttgt tctcgagctt
     2761 catggcgccg aaggtggtcg acatggaatc gatcaggctc ttcgcgagtt tcaattcgtc
     2821 cttgttggcc ttgtgcttct cgaccagcgg gacgtcctcg atcttacgga tttctttcgg
     2881 gtaacgcatc ttgtagagca cgatgccgtc gcccttgggc gcgatcatga ccacgtcctc
     2941 gcggtcgcgc agcaccacct tgcccacgcc cagcttgccg ctgtcgcgca gcgccgccga
     3001 cagcaggccg taggtcttgg cggccacgtc gccgtcgggg ccggcgtagt agggagagtc
     3061 gtagagagtg gcgtcgatct cggacgcgtc gacgaagcct tcgatgtcga tcaccttggt
     3121 gctcttgagc ttcaacttgt cgagatcgtc cttctcgaac acgacgtatt ggtcgggctc
     3181 gtactggaag cccttcagga tctcgtcgtt ctcgaccagg ttgttgcact tcttgcatcg
     3241 cttgtcgtaa ccgatgcggc cattgcattc gcggtgaatc tgattgaacg agatcgactc
     3301 cgccgtatcc accgcgttgt agacgcggat gggaatggtg acgagcgaaa agcggagatg
     3361 acctttccag atggatcgaa gggctgccat gatcgactcc ttgttgggcc gcgggtgagg
     3421 gtctcgcgac cgtatcaata tatagcttcc cgtaggcctt tcgcgcgtgt caaggcaagt
     3481 tagcggccta cattatatgg gagcgtcggc gggttgacgg caagatatgg gccgggctac
     3541 cgggctcgcc ggaccgactg ccccttggtg agctcggcct tcacgaccgg cttcccattc
     3601 acgatcaggt ccgtgaacgg agccggcaag ccctcggcgg ttttcagatc gggcccgtcc
     3661 tgcaggtggt aatgaatgtg cggctcgctg gtgttgcccg agttcccgca tagccccaag
     3721 ggagcgcccg ccttcacggg gtcgccggtt ttcaccttca gcgacttcgg ctgcatgtgc
     3781 gcgagcagcg agaactcgcc gttaccgtgg tcgatcacga ccccgttgcc gatcgggttt
     3841 ttggggtcca tctggccggg ttgctggtcg ggcagggagt cacacgacca caccacggtg
     3901 ccggccgcgg gggcgaagat ctcggcgccg tagctggagt agtccgcgag cgccttcccg
     3961 tcaccggcgt gagagacgcc gcccttcatg atgagcaggt cgtacgcgaa gcgctggctc
     4021 ttcgatatcg cgtggtagtt ctcgtcgatg gtccggccgc cccagaacac gtaccactcg
     4081 cccttgaacg gaagctcgag cgtggtcttc gcgacatagt cgagtttggt gctcgcgaag
     4141 ggttgcttct ccggctgcac gagcaggccg gatacgcgcg cgtcgtcgtc gaacgagagc
     4201 acgaggacca gcggcatagg cgccttctcg aagcggcagc gcgcgcggta cgtccacccc
     4261 atttcgcgca gcagtctttc gtcgatgacc tccgtgatgg ctccgacctg cccgtggatg
     4321 ctggccatga tggccgcaaa gcggagcgaa tcgcccatgg cctcgcgcat agggggggcg
     4381 aaggcggtcc acagcgcccc cggcgtcccg ctgctcacga acgggcgcag ctcgcgtgcg
     4441 cgcgccaacg ccgccgacga gtcgaagggg gcatcggcgg ccatcgcggg cgccggcaag
     4501 aacgagcaca agaccatcgc aaccaccgaa cgaagcatca ttcttccttt gccagagtga
     4561 cgtagaacat cagggagatt tcggccacga gccgtccgct gaccacgccg cgcgcgtccc
     4621 agtcttggat ggcgagcgag ccgtgctgcg cctcgacgat cttggcgccg ggcacgggcg
     4681 agccggcgtc gcgacgggtg gtcgtggccc gtaccaggct cgggcaccgc gccgacaggc
     4741 cggccagcac gatctcgtcg acgaacgtgc actcgtcgcc gcaccggccc ttgcgcacca
     4801 ggcgcagcgt gtcgccgatc gcctccgccg accactcgcg cgtctcgccg ctccagtctt
     4861 tgcgagccgc gttctccgcg tcgagtccga gcacggtgcc gcacgtgacc gcgacccctt
     4921 cgcgctcgct gccgttcgcg cggcagcccg cgatcacggc gaccgccgca accgcgcaca
     4981 agattctggc ccgagtactt gcatccatcg ttgacacccc tcggggcgga tgatagcatg
     5041 gcgacgctgt tttttcgacc cggaccatct agcccggcca catttcagcc cccggaggtt
     5101 cccgtcatga agcggttgtt cctcgttgtg gcagtactgg cgatttctgc cagcatccct
     5161 cgcaccagta tcgtggacgc cagcgcggac gaaccgctgt ggcgctgcgg taccgagctc
     5221 acacagtccg acatcgatca gttcaaagcc cgcgagcagc gcccgcgcaa cgggttcatc
     5281 ccgcagggtg cccccgcgcc gccgtactgc attcccatcg cggcgcacat cgtgcgcacg
     5341 tcggggggaa ccggcggtct gtcgctcgat cgcctcgacc aaggcatcgt cgactgcaat
     5401 accatgtacc agaacacggg catggtgttt cagctcctca gcgtcgacta catcgacgac
     5461 gacgattatt actacaacgt caataccgac gccgaaatcg acgccatgat tcaggagaac
     5521 gtggtcgcgg acgcgatcaa cgtgtacttc acgcccaacc tctcgaacga ggacggcggg
     5581 ttgtgcggcc gcggatcgtt cacgacctcg acgccgcagg gtgtcgcgat gaacaacggg
     5641 tgcaccggta cgtcggacaa tccctccacg ttcccgcacg agctggggca cttcttcgac
     5701 ctgttccaca cccacgagac cgcgttcggt gacgagctgg tggacgggtc gaactgcggg
     5761 agcgccggcg acaagctgtg cgacacgccc gcggaccccg gcctcgacga gggcaccaac
     5821 gtcaactcgt atccgagttg ttcgtactac gggggtgcga ccgacggcaa cggcgactcg
     5881 tacaatcccg acacgcatca gctgatgtcg tatgcaccca aagagtgccg cgacgtcatg
     5941 tcgccgcaga gcgagaccaa ggtcgtggcc acgctattga acgaacgccc cgagctgttg
     6001 aacaaggggt gcgcgccgac cgcgaacgcg ggcggcgata tcatcgcgga gtgcacgggg
     6061 ccgtcgctga cgcaggtcca gctcgacgga accggttcca gcgaccccga gggcggggca
     6121 ctgacgtact cgtggtcggc gacgggcatc gcgttcgacg acgacacgtc gtcgcagccg
     6181 accgggggct tccccttcgg caccaccacg gtgacgctca ccgtgagcga cggcaactac
     6241 acgcgcacgg atgaagtcaa cgttaccgtc gacgacacca cgccgccgga tatcacgtgc
     6301 ccgtcggaca tcacggtcga gtgcgagagc cactgcggca cgccggccac caacgccgcc
     6361 atcgccgcct tcctggccga cgtctccgcg accgacgtgt gcgacgcgag cctcaccatc
     6421 gagaacaacg cccccacctg cttccccgaa ggagcgaccg tggtgacgtt cacgaccgag
     6481 gattcgcacg gaaacgacat ctcgtgccag gccaccgtca ccgtcgaaga caccacgccc
     6541 ccggagatca ccgtggtgct cgatcgcgac gcgttgtggc cgcccaacca caagatggtc
     6601 gaggtgtgcg tcgacttcat cgaggtgacc gacatctgcg acgacgcgcc gacgtacgtg
     6661 ctgcaggaga tcacgtcgga cgagccggag aacgacgtcg gtgacggcaa caccgagccg
     6721 gacatccaag gcgccgactt cgagacgccg gacctgtgct tcgacctgcg cgcggagcgc
     6781 caagggaccg agaacggccg cacgtacacg atcgtgtatc gcgcggaaga cggtgccggc
     6841 aacgaggcct tcgcgaccgt ctacgtcgag gtgccgcacg accaatcggc caacgccatg
     6901 gcggcgagcg gttactcgcc cgagggcacc gagttcatcg gcgatcgctt cgcggtcgtg
     6961 attccgtcca cggacgggat tgacgcggcc gccatcgatc cgaaccgcgt ctacgtgggg
     7021 aacacccgtg gagcgacgcg cccggtggcc agtcgtctcg tggatgtaaa cgcggacggg
     7081 cgcgaggatc tcgcgctcga gttcccctcc aacgaagcga tgtcgtacat gccggtcttg
     7141 atgaacgtcg acccggaagc cggcggtctc cagagcattt acgacgggcc ggtgggagtc
     7201 cacttcaacg cgggtggcat cgactacctg gtttcggaca tcttcgcgct gggagaaccc
     7261 gtggagattc cgaccctggg cgccgtggcg ccgccgctcg tggtgaccaa cgacgtcgtc
     7321 tcgacgccgc gcgaaaccgg cttcaaggcg atccacccca acccgttcaa cccgcagacg
     7381 acggtgacgt tcacgttggc gtcgcccgag cacgtgcgca tcgcgatcta cgatgtgcgc
     7441 gggacgctgg tgcgccgctt ggaagaccgc gccatgcccg cgggcgagca ctcggtggtg
     7501 tgggacggac gggacgacgg aggccgcacg ggcagctccg gaatctattt cgtgcgcatg
     7561 ttggcgggtc ggaccgtcga gacgcgcaag atcgtcatgc tgaagtagcg aagcaagacg
     7621 aatcgtgagt cgaaacaggc tctccttcgg gggagcctgt ttcgttttct aacggctgcg
     7681 cgcgtggcgc gcgtcgcgga aaagatgcgg gaacccgcgc acaaagttgt acgggcagaa
     7741 gtcgcggacg gggctgaacg ggtgcttccc atacttgagg gcgacgaaga ggcggaacgg
     7801 gatcatgggt ccggtcgatc cggcgcgcag cgcgcgcgcc ggcgacagcg ccaccggccg
     7861 gtccttgcgc cccacgaata cggattgcgg ggcaatgccg aactcgggga cgtcgacggc
     7921 tatgcgctct gcgtaaaaat ccggcgcgcg catcacgtcg atcaggtcgc cgtcgtgggg
     7981 agcggtgagt accaggtaac cgcccgactt cagcacgcgc tggacctcgc ctatcgcttc
     8041 gatgtcgcgg ccggcgcgat cgaagacgtc ggctgccacg acgaagtcga acgtctcgtc
     8101 gtcgtagggg agttccgtgc cgcggcagtc gaacttcatg cggaagtaca tcgcgaggga
     8161 gccgcgcgtg cccgaatcca gcaggcgcgt cttcaggcga tacgtccacc ggaagaccgc
     8221 ctggtcctcg tcgaggacgc gcgcgaacgc gtgggcgacc gcgcggtcgc ggctggtcga
     8281 cccgcagttg cggcacaccg tcccctcgcg cccgcgatcg agacgcagga agcggtcgct
     8341 ccagccgcac acgttgcatc gaccggtcat acgacgatga taggctaggc ccggtcgggc
     8401 cgcaatcgca cgaacaccgg ctcgcgcagg gtgccgttcg gtgtgagcga cgcgtactgg
     8461 acctcgcacc acagcgtggg ctcgacccac acgctgatcg cgtcgtcgat tggcttttct
     8521 ttgaaggggc gtttggcttc gcgaagggcg cgcacctcgg cggccaccgc ctccagcgta
     8581 cgatcgtcga agccggtccc gactttgccc aggtacgccc acgcgtcgcc gcggcgctcc
     8641 gcgatgtgca gcgcgccgaa ggtcttcgcg cggtcgccct tgccctgggt gtacccgacg
     8701 acgagacagt ccatggtgcg cctcgtcttg accttgagcc acgcgtcgtt gcggcgaccc
     8761 ggcgtgtacg ggctcgtagc cttcttcgcc atgatgcctt ccaggccggc ctcgcgcgcc
     8821 gcctcgaaca acgcgtcccc gtcttcgacc gtctggctca cgcggtaggc cgactctttc
     8881 ttgatcgcgt cggccagcca ctcgcgccgg cgcgtgaggg gctcgttcat gagcgggcgc
     8941 ccgtcgaggt agaggcaatc gaagacgtag cacaccgcgg ggtgtttcga cttcgcgcgc
     9001 gcgaccgctc cttcgctggt ctggcgcatg cgattgatga cgtccttgaa gagggggcgg
     9061 ccggcggagt ccaggcacac gatctcgcag tcgaacagcc cgcacgtggc gcgaaacgaa
     9121 cccgggtcgg cgagctccgg gaacgccacc gtgatgtcgt tctggttgcg cgagcgaatg
     9181 cgcaccacgc cctcgtcgag cgcgatcatg gcgcggatac cgtcccactt gacctcgtag
     9241 atccagtcac caaccgaggg caccttgtcc gacagatcgg ccagcatgaa ctcgaccggt
     9301 tcgaccagcc agtcggtctg gggtgcgtcg acgcgttcga gcaggcactc gttgccgcgc
     9361 gtgggaatga tgcggtactc gccggtcaag ccgcggccgc tcaggcgcac gtaggtgctg
     9421 tctttcttcg ccttggtgac ttcgtacttg ccggacgcga acacccacat gtcgccgccg
     9481 ccgtattctc ccttggggat ggtcccctcg aagttgagat actcgatggg gtggtcttcg
     9541 gtttgcacgg cgagacgctt gatgccgggg cgcggcggga gccccttcgg gaccgcccac
     9601 gatttcaaga cgccgtcctt ctcgagacga aggtcgtagt gcagccgcga ggcgtggtgg
     9661 cgatgcacga cgaagcggtt ggtgccttct tcgagcggct ggggtgcggg ctcgggcgtc
     9721 ttcgagaact cgcgcttctt ctcgtacgac ttcagctgct cgggagtctt gcgcttaccc
     9781 gacttctcga gcttcttcgt gggttttggc gcgctgcggt gggtgtgcag ctcgaccgca
     9841 tacgcctcca tcgcctccca cgggtcgccg tcgcgcttga cgatgtcggg cacgttgcgc
     9901 agatgaaaat cgcggatccc gtccatggtg tggagctgct cccatttgag cggcatcgac
     9961 accgtggctc ccggaagccc gcgcacgctg tacgccgcca cgatcgtctg cgaggggcga
    10021 ttgcgataga tatcgatcag aatcttgttg tcgcgttgct ccttgtggat tcggagtgtg
    10081 cacgtcttgg aattggcagc gacgaagggc ttcgcgatcg attcgcacgc cttgaacacc
    10141 gtgtcgaagt cccacttggg ctcgatgggg gcgacgacgt gcagcccctt gcgccccgtc
    10201 gtcttgacga aggcgtggta gccgtgctgc tcgaaggcgg cgcgcagccc ctcgccgacc
    10261 tcgacgatct gcgcccacgg ggtcccgtcg ggcgggtcga cgtcgaagac gaagtagtcc
    10321 gggtggtcgc ggttcttcgc gcggcagtgc atctggtgca gctcgatgca cgccatgttg
    10381 gcgagccaca ccaaggacgc ctcctcggtg gcaatcacgt agttgatggt cttcttcgcg
    10441 tccccgatct ccacgctctc cagccagtcc ggcacccatc cgggcaggtt cttctggaag
    10501 aaggtctcgc cgtggatgcc gtcgggaaag cgaaccagcg agagcgggcg gcctcgaatg
    10561 tgcgttagaa tggtgggcgc gatagcgagg tagtattgga tcaactcggc tttgaggacg
    10621 ccgtcctccg gccacagaac cttgttgagg ttcgacagct cgagcgtgcg cttgccgact
    10681 tgaacgactt gggacgagcg aggcatactg gctcattata gcagggagcg atcgatgaat
    10741 ctctacgccg gtaccagcgg gttctcttac aaagagtgga agggtcactt ctatccggag
    10801 aagatcgcgc ccgaccagat gctgaacttc tactcgacca aactccccgc ggtcgagatc
    10861 aacaacacct tctatcgctt gccgcgccgc gaagcgctcg aggcctgggc ggcgcaagtg
    10921 ccgcccgact ttcgcttcgc cgtgaaagcg tcgcagaaga tcacgcacat gaagcggctg
    10981 aagaacgcgg acgacgagac gggctatctc atggacgtcg tcggcgcgat ggagaagaag
    11041 ctcggggtgg tgttcttcca gctcccgccc aacttcaaga aagacgtcga gcgattgggc
    11101 gcattcctag gaaca
//
LOCUS       DASPGK010000125         6622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_32700, whole genome shotgun sequence.
ACCESSION   DASPGK010000125 DASPGK010000000
VERSION     DASPGK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32700"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VEC56_03610"
     CDS             <1..492
                     /locus_tag="VEC56_03610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV13272.1"
                     /translation="PDKAPVTVQNFLYYVDNKFYDGLIFHRVIPDFMIQGGGFTPEMA
                     QKQGKPAIEIESANGLTNDKYTVAMARTSDPNSATSQFFINVKDNAFLNFKSKTPQGW
                     GYAVFGKVTSGQQVVHAIEKVKTGQKNGMNDVPVEVVKITKAYRKGAAPAKEAAKTPD
                     NKG"
     gene            complement(562..1359)
                     /locus_tag="VEC56_03615"
     CDS             complement(562..1359)
                     /locus_tag="VEC56_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV13273.1"
                     /translation="MKRVGAVARMTLRRYAKSRLLHVAGIVAILVLGAPTAYLPNVIA
                     AARRGDAEAVQTAVEVMLNMAVSFFMILVAFIATAVGSTCVRRDLVDGTIYGVLSKPL
                     SRTEYMVGTLLGGAAVVVFSWLVFGGALTALAALATDPLGPPEYAMLAGRCLQIVTLM
                     CMATLLSTRFGPWVAGPLALLAFYGRETIDRLAALFELPGVVVDILKFPFPVLKALGA
                     ITERVSRSDFDPAPVLPGVIHVIDYILVMVVLACLSFRRLDLNRTSD"
     gene            complement(1356..2243)
                     /locus_tag="VEC56_03620"
     CDS             complement(1356..2243)
                     /locus_tag="VEC56_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV13274.1"
                     /translation="MTPVLEARGLSKTYASRFPRREFTALDDVTFEVQRGEILAFLGP
                     NGAGKTTAINLLLGFVKPTRGEPRLFGGDPADPRSRSRLGYLPENYAFYASFTAPVLL
                     HYFGQLCGIPRMERAQRIDALLDRVGLADARDRPVGKYSRGMRQRLGIAQALLNHPEL
                     LILDEPTSGFDPLGRLMVRDLLVDMKRAGASILLSSHILSEVEAVCDRVVILNRGRVV
                     REGTLGDVLGRGGGFEVAFRDANGQESTIVVESERDAQHVLDDVRRAGGVVVRYRPVG
                     RSLEEVFLEDVGSNEGGDA"
     gene            complement(2227..2787)
                     /locus_tag="VEC56_03625"
     CDS             complement(2227..2787)
                     /locus_tag="VEC56_03625"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV13275.1"
                     /translation="MAPRWIVALFLLASLGVQYWAHSRYENSGPTLNVEEGMAAPAWS
                     LADVDGNRVSSQDFRGRILILDFWATWCGPCRSEFNMLRAWWKNGEETGLLNGVELVA
                     VNLGEPLALVNRFIENRDLPFRFVLDSDGAVAGAFGVTSLPSLIVIDPDGRVAYTSIG
                     YHAAIGVNLSRTLSEIEKERRRDPRP"
     gene            complement(2806..3450)
                     /locus_tag="VEC56_03630"
     CDS             complement(2806..3450)
                     /locus_tag="VEC56_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13276.1"
                     /translation="MKKWFGILGALTALAGCQDSTSDVAVVAHEELAAAPDTVIVSDG
                     LTVEMYLPDNPLAIELDARLQANAGVQMGLDLMADEGFTRDGTAYYLRGVGVDGRALE
                     ATWFPVTDATGERVSLLGHVRTDTNEFVIPVRSLDPGEPAANVGLLARGKNGQWRPST
                     SSFESCLSFYQALFVACYGACIESGIKSDACYRSCRVTVMVAFVACIFFATADS"
     gene            complement(3732..4094)
                     /locus_tag="VEC56_03635"
     CDS             complement(3732..4094)
                     /locus_tag="VEC56_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13277.1"
                     /translation="MLYESKKHALLTRRRFVDRLAKHAVVAAIIVVASLAIGIAGYME
                     FEHLSLLDALLNSAMLLGGMGPVDLPRTTGGKLFASCYALYAGFVILAVVGIVLAPVA
                     HRILHRFHLADEEGTRGK"
     gene            complement(4094..6118)
                     /gene="ligA"
                     /locus_tag="VEC56_03640"
     CDS             complement(4094..6118)
                     /gene="ligA"
                     /locus_tag="VEC56_03640"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXV13278.1"
                     /translation="MPAHKPAKPSRAEVERLRREIEEHNYTYYVLDQPTVSDAEYDTM
                     LRRLQEIEATWPEFRTSDSPTQRIGAPPAEGFAVVTRAVPMLSLENAMAEEEFREWRE
                     RLIRVVGPAGETDYVCEPKMDGVAVELVYEDGTLVQGSTRGDGVNGEDVTPNVRTIRA
                     IPLRLMHPARGAKPPKTLSVRGEVYMPLADFERINKKQMESGEKLYANPRNTAAGSLK
                     QLDPRITATRPLKFFAYGIGSVEGTPIRSQWSLLETLKAWGLPVNPLSKRCASVEDVI
                     AFHNTLEEKRPKLPYEIDGAVVKVNDFGVQEEAGTRSRSPRWAIAWKFPPQEARTRVL
                     DIEVQVGRTGALTPVARLEPVRVGGVTVSNATLHNADEIEKKDVRVGDWVFVRRAGDV
                     IPEVVAPVKELRKGSLPKFKMPEKCPVCGTRVVRPEGEAVTRCPNFACPIQVRGRLIH
                     FVSRGAIDIEGLGYKLIDQLVTAGLVETPADFYRLTADDLIPLERMAEKSAKNVIGAI
                     ERSKHTTLPRFIYALGIRNVGETVAEVLAENFPSMDALMDASEEELSSIHGVGDVIAQ
                     EIRAWAAVASHRKLVRELLKAGIAFARAPRAVSNEFAGKTFVFTGTLTKLTREEAEGE
                     VKKRGGKAASSVSKNTDYVVAGEKAGSKLEKAQKLGVEVIDEDAFLRMIR"
     gene            6196..>6622
                     /locus_tag="VEC56_03645"
     CDS             6196..>6622
                     /locus_tag="VEC56_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13279.1"
                     /translation="MSKQRTAPPSTEKPLVGELERIGPDGRDAVVVGVEAPGFDDLSA
                     REKRFLYLMTRAAIAGNGIAFQQSHRDADDIVRLLEACFAHADGLDPAVRDALHEHLK
                     LVWIHHGPYHHHTHAKFVPSRLTPAMLTAAARSAASHGAI"
BASE COUNT         1220 a         2242 c         2093 g         1067 t
ORIGIN      
        1 cccgacaagg cgccggtcac ggtgcagaat ttcctctact acgtggacaa caagttctac
       61 gacggcctga tcttccatcg cgtgattccc gacttcatga tccagggcgg cggtttcacg
      121 cccgaaatgg cgcagaagca gggcaagccg gccatcgaga tcgagtccgc caacggcttg
      181 accaacgaca agtacacggt cgcgatggcg cgaaccagcg accccaacag cgccaccagc
      241 cagttcttca ttaatgtcaa ggacaacgcg ttcttgaact tcaagtccaa gacgccgcag
      301 ggctggggtt acgcggtgtt cggcaaggtc acgtcgggcc agcaggtcgt gcacgcgatc
      361 gagaaggtca agacgggaca gaagaacggc atgaacgacg ttccggtcga agtcgtcaag
      421 atcaccaagg cctaccgcaa gggggccgcg cccgcgaagg aagccgcgaa gacgcccgac
      481 aacaagggct agtcgttccc gacggttaga attgctcgcg aagagccgcg tccggccgag
      541 ccgggcgcgg cttttccttc ttcagtccga cgtgcggttg aggtcgagac gccggaagga
      601 gaggcacgcg agaacaacca tcacgaggat gtagtcgatg acgtggatga cgccggggag
      661 gacgggggcg ggatcaaaat cgctcctcga cacccgttcg gtgatcgcgc ccagcgcctt
      721 caagaccggg aacggaaact tcaatatatc cacgaccacg ccgggcagtt cgaagagcgc
      781 ggcgagacgg tcgatcgtct cacggccgta gaacgcgagc agcgcgagag gtcccgccac
      841 ccaagggccg aagcgcgtcg acagcaacgt cgccatgcac atgagcgtca cgatttgcag
      901 gcaacggccc gccagcatcg catattcggg cgggcccagc ggatcggtcg ccagcgcggc
      961 cagcgcggtc aacgccccgc cgaacacgag ccacgaaaat acgacgacgg ccgcgccgcc
     1021 gagcagcgtc cccaccatgt actccgtgcg ggaaagcggc ttcgacagca caccgtatat
     1081 cgtcccgtcc acgaggtcgc ggcgcacgca agtcgatccg acggccgtcg cgatgaacgc
     1141 gaccaggatc atgaagaagg atacggccat gttgagcatt acctcgaccg cggtctgaac
     1201 cgcctccgcg tcgccgcggc gagcggccgc gatcacgttg ggcaggtagg ccgtcggagc
     1261 gccgagcaca aggatcgcga cgatgccggc gacgtgcagc aggcggctct tggcgtagcg
     1321 tcgaagcgtc atccgggcca cggcgccgac tcgtttcacg cgtctcctcc ttcgttcgat
     1381 ccgacatcct ccaggaacac ctcttcgaga gaccggccga cgggccggta gcggacgacc
     1441 acgccgcccg cacgacgaac gtcatcgagc acgtgctgcg cgtcgcgctc gctctccacc
     1501 acaatcgtgg actcctgccc gttcgcgtcg cggaacgcga cctcgaaacc ccctccgcgc
     1561 cccaacacgt ctccgagggt gccttcgcga acgacgcggc cgcgattgag gatcaccacc
     1621 cggtcgcaga cggcctcgac ttcggagagg atgtgcgacg acaagagaat cgacgcaccc
     1681 gcgcgcttca tgtcgaccag caggtcgcgc accatcaggc ggcccagcgg atcgaagccg
     1741 gacgtgggct cgtcgaggat caggagctcg ggatggttga ggagcgcctg cgcgatcccc
     1801 aaccgctgac gcatcccgcg agagtacttc cccaccggcc gatcccgcgc gtccgcgagc
     1861 ccgacgcggt cgagaagcgc gtcgatccgc tgcgcgcgtt ccatccgcgg aatcccgcac
     1921 agctgcccga agtagtggag cagcaccggt gcggtgaacg acgcatagaa cgcatagttc
     1981 tcgggaaggt acccgaggcg cgagcgggag cgcgggtcgg ccgggtcgcc gccgaacagc
     2041 ctgggctccc cgcgcgtggg cttgacgaac ccgagcagca gattgatcgc cgtcgtcttg
     2101 cccgcgccgt tggggccgag aaacgcaaga atctcgccgc gctgcacctc gaaggtgacg
     2161 tcgtcgaggg cggtgaactc ccggcgggga aaccgcgacg cgtaggtctt ggacagaccg
     2221 cgtgcctcaa ggacgggggt cacgccggcg ctccttctcg atctcactga gcgtgcgcga
     2281 aaggttgacc ccgatcgccg cgtggtatcc gatgctcgta taggcgacgc gtccgtccgg
     2341 gtcgatcacg atcaatgagg gcagcgacgt cacgccgaag gcgcccgcca ccgcgccgtc
     2401 gctgtcgagc acgaatcgaa acggcagatc ccggttctcg atgaatcgat tgaccagcgc
     2461 cagaggctct cccaagttga cggccaccaa ctcgacgccg ttcaagagcc ccgtttcctc
     2521 cccgttcttc caccacgccc gcagcatgtt gaactccgaa cggcacggtc cgcaccacgt
     2581 cgcccagaag tcgaggatca ggatgcggcc ccgaaagtcc tgcgatgaaa cccggttgcc
     2641 gtccacgtcc gcgagcgacc acgcgggggc cgccatgccc tcctcgacgt tcagcgtcgg
     2701 gcccgagttc tcgtatcggg agtgggccca atactgaacg ccgagggacg cgagcaggaa
     2761 caatgcaacg atccagcgtg gggccatgag atcgacgtga cccgtttagg agtccgccgt
     2821 ggcgaagaag atgcacgcca cgaaggcgac cattaccgtc acccggcaac tccgataaca
     2881 cgcatccgat ttgattccac tttcgatgca cgcgccatag cacgccacaa acagggcctg
     2941 gtagaatgac aagcaacttt cgaaagagct tgtactcgga cgccattggc cgttcttgcc
     3001 ccgcgccaac agtccgacgt tcgctgccgg ctcgccggga tcgaggctcc taacggggat
     3061 tacgaattcg ttcgtgtccg tacgaacgtg tcccaatagc gagacacgtt cccccgttgc
     3121 gtccgtgacc ggaaaccacg tcgcctcgag cgcgcggccg tcgacgccca cgccgcgcaa
     3181 atagtacgcg gtaccatcgc gcgtgaaacc ctcgtcggcc atgaggtcca atcccatctg
     3241 cacgcccgcg ttcgcctgca agcgagcgtc gagctcgatc gccaacggat tgtccggcaa
     3301 atacatctcg accgtcagac cgtcactgac aatgaccgta tcgggcgcgg cggcgagttc
     3361 ctcgtgcgcg acgaccgcca cgtccgacgt cgaatcctgg caccctgcga gagccgtcaa
     3421 cgcgcccaga atcccaaacc acttcttcat cacagcctcc cttccacacc acccaaaaga
     3481 agcccggcgg cgtatccgcc gttgcgacga gagaagcgca cgcgacgtgc caggggaccc
     3541 gcgcgtgatc taatccgttc tgcgattgcg gattggagcg tttgtggcgg tggtggcggg
     3601 gaaccctgag aagggactcc gaagcgacga tgcaatctct ggaaacccgg ccaagtcgcg
     3661 cgggaacgcg acgaagcgag atgtctccgg cgtggacggg cgtgtcgacg gatcgacagc
     3721 ggccgttgag gtcacttgcc gcgtgtgcct tcctcgtcgg cgaggtggaa acggtggagg
     3781 atgcggtggg cgacgggggc caggacgatg ccgaccaccg cgaggatgac gaaccccgcg
     3841 tacagcgcat agcacgacgc aaacagcttg ccgccggtcg tgcgcggcaa gtcgacgggt
     3901 cccatgccgc cgagcagcat ggcggagttc agcagcgcgt cgaggagact caagtgttcg
     3961 aactccatat aacccgcgat accgatcgcg agcgatgcca cgacgatgat cgccgctacg
     4021 accgcgtgct tcgccaggcg gtccacgaac cggcggcgcg tgagcagcgc gtgcttcttg
     4081 ctctcgtaca acatcagcga atcatgcgca agaacgcgtc ctcgtcgatg acctcgacgc
     4141 ccagcttctg cgccttctcg agcttcgagc ccgccttctc acccgcgacc acgtagtcgg
     4201 tgttctttga taccgatgac gccgccttgc cgccgcgctt cttcacttct ccttcggctt
     4261 cttcgcgcgt gagtttggtg agggtgcccg tgaaaacgaa ggtcttcccc gcgaactcgt
     4321 tgctcacggc acgcggcgcg cgcgcgaagg cgatgccggc cttgagcaat tcgcgcacga
     4381 gcttgcgatg ggacgcgacc gccgcccagg cgcggatttc ttgcgcgatg acgtcgccca
     4441 cgccgtggat gctcgacagc tcctcttcgc tcgcgtccat caacgcgtcc atggacggga
     4501 agttctccgc gagcacctcc gccaccgttt cgccgacgtt gcgaatgccg agcgcataga
     4561 tgaaacgcgg gagagtcgtg tgcttcgagc gctcgatggc gccgatcacg ttcttcgccg
     4621 acttctccgc catgcgctcg aggggaatca gatcgtcggc ggttagccgg tagaaatccg
     4681 cgggcgtctc gaccaacccg gccgtcacca gctggtcgat cagcttgtag ccgaggcctt
     4741 cgatatcgat ggcgccgcgc gacacgaagt ggatcaagcg cccgcgcact tgaatggggc
     4801 acgcgaagtt ggggcagcgc gtgacggcct cgccctcggg acgcacgacg cgcgtgccgc
     4861 acacggggca cttctccggc atcttgaact tggggagtga gcccttgcgc agctctttga
     4921 ccggcgccac cacttccggg atgacgtcgc ccgcgcggcg cacgaacacc cagtcgccca
     4981 cgcgcacgtc cttcttctcg atctcatcgg cgttgtggag ggtggcgttc gataccgtca
     5041 cgccgcccac gcgcaccggc tccaggcgcg cgaccggtgt caaggcaccg gttcgcccga
     5101 cttgcacttc gatgtcgagc acgcgcgtgc gcgcttcctg gggcgggaac ttccacgcga
     5161 tcgcccagcg cggcgagcgc gagcgcgtcc ccgcttcttc ctgtacgccg aagtcgttga
     5221 ccttcacgac ggcgccgtcg atctcgtacg gcagcttggg ccgcttctct tccagcgtat
     5281 tatgaaacgc gatcacgtcc tcgaccgacg cgcagcgctt cgacaacgga ttgacgggaa
     5341 gcccccacgc cttgagcgtc tcgagcagag accactgcga ccggatcggc gtcccttcga
     5401 cgctcccgat tccgtacgcg aagaacttga gcggccgcgt cgcggtgatc ctggggtcga
     5461 gctgcttgag cgagccggcc gcggtgttgc gcgggttggc gtaaagtttc tccccgctct
     5521 ccatctgctt cttgttgatg cgctcgaagt cggcgagcgg catgtagact tcgccgcgca
     5581 cgctcagcgt cttgggcggc ttggcaccgc gcgcggggtg catgagacgc agcggaatgg
     5641 cgcggatggt gcgcacgttg ggcgtgacgt cttcgccgtt cacaccgtcg ccgcgcgtcg
     5701 agccctgcac cagcgtgccg tcttcgtaca ccaactcgac ggcgaccccg tccatcttgg
     5761 gttcgcacac gtagtcggtc tcgcccgcgg gaccgaccac acggatcaag cgctcgcgcc
     5821 actcgcggaa ctcctcctcg gccatcgcgt tctcgagcga tagcatgggg acggcgcgcg
     5881 tgacgacggc gaagccctcc gccggcggag cgccgatgcg ttgggtcggc gaatcggaag
     5941 tccggaactc cggccacgtg gcctcgatct cctggaggcg gcgcagcatc gtgtcgtact
     6001 cggcgtccga aacggtgggc tggtcgagca cgtaatacgt gtagttgtgc tcctctattt
     6061 cgcggcgaag cctctcgact tcggcgcgag agggcttggc gggcttgtgc gcgggcatac
     6121 ggcttgctcc gtcgtccatg gcgggattct agcagagggc cgccgaagag tgtacaatcg
     6181 agccggcttt ccactatgtc gaagcaacgc accgcacccc catcgaccga gaagccgctg
     6241 gtcggcgagc tcgagcgcat cggccccgac ggccgcgacg ccgtcgtggt cggcgtcgaa
     6301 gcgcccggct tcgacgatct gtcggcgcgc gagaagcgct tcctctatct aatgacgcgg
     6361 gccgcgatcg ccggcaacgg gatcgcgttc cagcagagcc accgcgacgc cgacgacatc
     6421 gtgcgtttgc tggaggcgtg cttcgcgcat gccgacgggc tcgaccccgc ggtacgcgac
     6481 gcgctgcacg agcacttgaa gctggtctgg atccatcacg gtccgtacca ccaccacacc
     6541 cacgccaagt tcgtgccgtc tcgactaacc cccgcgatgc tcactgcggc cgcgcgcagc
     6601 gcggcttctc atggtgcgat ac
//
LOCUS       DASPGK010000126         5696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1087419, whole genome shotgun sequence.
ACCESSION   DASPGK010000126 DASPGK010000000
VERSION     DASPGK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087419"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VEC56_03650"
     CDS             complement(<1..411)
                     /locus_tag="VEC56_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HXV13280.1"
                     /translation="MAVYAVGITGGSGAPYALRLLGALTASGHDAHVVISPAGEKVLQ
                     LETGMRLGRTLREKEEALTRALEIGGEARGTLRLFDPMNLAAPISSGSFPSAGMVIVP
                     CSTGTLGRVANGISSNLIERAADVMLKERRRLVVV"
     gene            complement(413..1261)
                     /locus_tag="VEC56_03655"
     CDS             complement(413..1261)
                     /locus_tag="VEC56_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HXV13281.1"
                     /translation="MRSLREFLDTIRVEHTIFALPFAYATLFLTSDGWPTASNAIWIT
                     VAMVAARTVGMAVNRIIDAEVDARNPRTASRAIPAGRLHSSRALAFTAVALAFFIVAV
                     WQLHPICRMLWPIVVAVMVAYPYAKRVTPLAHLVLGLVYFMVPTSVWLAVTGELSSAS
                     LFLGVGAGAWVAGFDIIYACQDVEVDRREKLHSIPADFGVARGLAVAFGLHAAFVVCL
                     VAAGSILSAGRPYYIGVTLTTVLLLYEHRLVKPHDLSKVNAAFFTTNGVVSIALFALI
                     ALDTVI"
     gene            1455..2234
                     /locus_tag="VEC56_03660"
     CDS             1455..2234
                     /locus_tag="VEC56_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13282.1"
                     /translation="MERTRTRSIQGFILDHVDGDPRGIARRVAQAYGISRQAANRHLD
                     ALVQTGLLDEAGHTRARSYALRRTSAVSREFRVTPVLGAERVWSEHAAPVLAGDRAAL
                     RDACRAVFKELLENAVAHAGASWITFELATTAREVDLTVSDDGRGVFVSLAEKLRVAT
                     PREAVEEFARLARMRSMESPAVKLMLLARGTERFTLASSGVSCEFDAARDLWSVREDE
                     ATRAGTSASCRFRRTPAGSAHTRGSRESSGSARTHPPLRPR"
     gene            complement(2173..2952)
                     /locus_tag="VEC56_03665"
     CDS             complement(2173..2952)
                     /locus_tag="VEC56_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV13283.1"
                     /translation="MSLLGALESLGLAQIEALSYAFLAGSSPTTGYKVARGIGKPTAN
                     VYRALESLERKGLVVQDRGTPPAYRALPPDDMLARLEREFLEKKARAARELAALKPGV
                     DDEKLYALKLQDQVIARAHLLLAAARRVVLMEAPTASLVGLESALDDARSRGIRVVIV
                     TDHQPAVPHDDSIVSERRAIKATRMVADARDSLISTLPNDAGGVREAIWTRSPLVGRM
                     LHDALVSEVFYGLVERSLEDGISVDEVEDAFERCRRIRESL"
     gene            complement(3099..3518)
                     /gene="msrB"
                     /locus_tag="VEC56_03670"
     CDS             complement(3099..3518)
                     /gene="msrB"
                     /locus_tag="VEC56_03670"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXV13284.1"
                     /translation="MSKINKTEKEWKEILSPDAYRVAREKGTEPAFTGSYWNNHDAGV
                     YVCVACGQPLFASDTKFDSGTGWPSFYEPVDQRNVEMEGDGSHGMQRTEVLCSRCESH
                     LGHVFDDGPRPTGLRYCINSVSLKFVPKDAKGDPSKK"
     gene            complement(3627..4349)
                     /locus_tag="VEC56_03675"
                     /pseudo
     CDS             complement(3627..4349)
                     /locus_tag="VEC56_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
     gene            complement(4331..5059)
                     /gene="pepE"
                     /locus_tag="VEC56_03680"
     CDS             complement(4331..5059)
                     /gene="pepE"
                     /locus_tag="VEC56_03680"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HXV13285.1"
                     /translation="MSNATQRLLLLSNSRNFGGGYMEHADAWMRAFFAGTRSAAFVPY
                     AGVTVEWDEYERMVAERFAPLGIEVRSIHHAKHPAQEIETAEAVLVGGGNTFHLLKHL
                     YDTGALWAIRARVLSGTPYMGWSAGSNVACPTIKTTNDMPVVEPARFDATRLIPFQVN
                     PHYTDATLPNHGGETRAQRLAEFTKSNPSIYVVGLREGSAVEVSNGTTKLLGPHSARI
                     FFGSREPVEHAPGASMEFLWNVTT"
     gene            5155..>5696
                     /locus_tag="VEC56_03685"
     CDS             5155..>5696
                     /locus_tag="VEC56_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13286.1"
                     /translation="MPKRPGLPLAESIRFGWRTVREYPLFLVGTILVAIGVPSLVEWG
                     GRVALDDGAPQLAIQVISMAVSATLTLGLAKIYLRFRDGERPVFENLFDGLAHFHKYL
                     GASIIVFFAVIMGLVLLVVPGIVFLIRLWFLGFVIVDTKAGPLEAVQQSWDISRGYTT
                     DLLVLFFILVGVNLLGLICLG"
BASE COUNT          970 a         1892 c         1829 g         1005 t
ORIGIN      
        1 caccaccacg aggcgtcgtc gttccttgag catcacgtcg gcggcgcgct cgatcaagtt
       61 cgacgagatg ccgttcgcga cgcgaccgag cgtgccggtc gagcacggca cgatgaccat
      121 ccccgcgctc gggaacgatc cgctggagat gggcgcggcc aggttcatag ggtcgaagag
      181 acgcagcgtt ccacgtgctt ccccgccgat ttccagcgcg cgcgtaaggg cctcttcttt
      241 ttcgcgcagc gtgcgaccca gtcgcatccc ggtctcgagc tgcagcacct tctcccccgc
      301 cggcgagatg acgacgtgcg cgtcgtgccc ggacgccgtc aacgcgccca gcagtcgcag
      361 cgcgtacggc gccccgcttc cccccgtgat gccgaccgcg taaaccgcca cgctagatca
      421 ccgtgtccag ggcgatgagt gcgaacagag cgatgctcac gacaccgttg gtcgtgaaga
      481 acgccgcgtt caccttggag aggtcgtggg gcttgacgag tcggtgctca tataggagca
      541 gcactgtcgt gagcgttaca ccgatgtaat acgggcgtcc cgccgacagt atcgaccccg
      601 ctgcaacgag gcacacgacg aatgccgcgt ggagtccgaa cgcgaccgcg agtccgcgtg
      661 ccacaccgaa gtccgcggga atcgagtgca gtttttcgcg ccggtcgacc tccacgtcct
      721 ggcacgcata gatgatgtcg aagccggcca cccatgcgcc cgcccccacc ccgaggaaca
      781 gcgaggcgct cgacagctcg ccggtgaccg ccagccacac ggaggtcggc accatgaagt
      841 acaccagccc caggaccagg tgggctaagg gagtcacgcg ctttgcgtat gggtaggcga
      901 ccatcacggc gaccacgatc ggccagagca ttcgacagat ggggtggagc tgccagaccg
      961 ccacgatgaa aaatgcgagg gccaccgcgg tgaacgcgag ggcccgcgag ctatggagtc
     1021 gcccagccgg aatcgccctc gacgcggtac gcggattacg agcatccact tcggcgtcga
     1081 tgatccgatt gaccgccatc ccgacggtac gggcagccac catcgcgacg gtgatccaga
     1141 tcgcgttcga tgcagtgggc cagccgtcgc tcgtgaggaa cagggtggcg tacgcgaagg
     1201 ggagcgcgaa gatggtgtgc tcgacccgaa ttgtatcgag gaattcgcgg aggctacgca
     1261 ttcagataac cttttgtaaa agaatcgagt agttatctaa agttgacaat agtccggctc
     1321 aacaagttga caatcctcgc ggcccaatgt tgacaatatg gcgtggttaa tgttgacaat
     1381 tccgctgcct gccttggccg attcacggca aaacttgttg acaatatccg gggcccgcgc
     1441 ctaaacgcaa actcgtggaa cggacccgaa ctcgatcgat ccagggcttc attctcgacc
     1501 acgtcgacgg ggatcccagg gggattgccc ggcgagtcgc gcaagcatac gggatttccc
     1561 ggcaggccgc aaaccggcac ctcgacgccc tggtgcaaac cggcctcctg gacgaagcgg
     1621 ggcacacgcg ggcgcgatcc tacgcgttgc ggcggacgtc ggcggtctcg cgggagttcc
     1681 gcgtgacccc ggtgctgggc gccgaacgtg tctggagcga acacgcggcg cccgtgctgg
     1741 cgggggatcg ggccgcgctt cgcgacgcgt gccgcgccgt cttcaaggag ctgctcgaga
     1801 acgccgtcgc gcacgcgggt gcgtcgtgga tcaccttcga gctcgccacc accgcgcgcg
     1861 aggtcgatct gacggtctcc gacgacggcc gcggagtctt cgtctcgctc gcagagaagc
     1921 ttcgcgtcgc gactccgcgc gaggcggtcg aagagttcgc gcgccttgcg cgcatgcgct
     1981 cgatggagtc gcccgcggtc aaactgatgc tcctcgcgcg cggcacggag cgcttcacgc
     2041 tcgcgtcctc gggggtgtcg tgcgagttcg atgcggcgcg cgatctctgg agtgtgcgcg
     2101 aagacgaggc cacacgcgcg gggacgtcgg cttcgtgccg attccgtcgc acgcccgcgg
     2161 gatcggcgca cactagaggc tctcgcgaat cctccggcag cgctcgaacg catcctccac
     2221 ttcgtccacg ctgatgccgt cctcgagcga tcgctcgacc agcccgtaga acacctccga
     2281 aaccagcgcg tcgtgcagca tgcgtcccac cagggggctg cgcgtccaga tggcttcgcg
     2341 cacacctccg gcgtcgttgg gcagggtgga gatgagcgag tcgcgcgcgt ccgccaccat
     2401 gcgtgtggcc ttgattgcgc gtcgctcgct cacgatggag tcgtcgtgcg ggactgcggg
     2461 ctggtgatcg gtcacgatga cgacgcggat cccgcgcgac cgcgcgtcgt cgagcgcgga
     2521 ttcgagtccc acgagcgacg cggtgggcgc ctccatcaag accacgcggc gtgcggctgc
     2581 cagcaggagg tgggcgcggg caatcacctg gtcctgcaac ttcagcgcgt agagcttttc
     2641 gtcgtccacg ccgggcttga gggccgcgag ctcgcgcgcg gcgcgcgcct tcttttccag
     2701 aaactcgcgc tcgagtcgcg cgagcatgtc gtcggggggg agtgcgcgat acgccggggg
     2761 cgtcccgcga tcctgcacca ccagcccctt ccgctcgagc gactcgagcg cccgatagac
     2821 gttcgcggtc ggcttgccga tcccgcgcgc gactttgtac ccggtcgtgg gggaactccc
     2881 ggccaggaag gcgtaggaga gagcctcgat ctgggccaaa ccaagggact ccaaggcacc
     2941 gagcaagctc atagtagcgc agactactat cagactgagc gaccggtcaa gcgcctatat
     3001 cgagttagct gctcaagcta ctatcaaaac aaatgcgcgc ttaactgaaa gatgcacaga
     3061 caattagatc gttggcgtcc cgtgagggac gcgaagggct acttcttcga cgggtcgccc
     3121 ttcgcgtcct tggggacgaa cttcagcgat acggagttga tgcagtaccg gagcccggtg
     3181 gggcgggggc cgtcgtcgaa gacgtggccc aggtgggact cgcaccggct gcagagcacc
     3241 tcggtgcgct gcatcccgtg gctgccgtca ccctccatct cgacgttacg ctggtcgacc
     3301 ggctcgtaga agctcggcca ccccgtcccg gagtcgaact tggtatccga agcgaacaag
     3361 ggctggccgc acgcgacgca cacatagacg ccggcatcgt ggttgttcca atacgaaccg
     3421 gtaaacgccg gctcggtccc cttctcgcgc gccacccggt acgcgtcggg ggagaggatc
     3481 tccttccatt ccttctcggt cttgttgatc ttcgacatag tggtcctttc gtcttttcgt
     3541 tgcagcgcgg cgaccgcggt ggtggtgagc catccacccg ccgcttgcag gggctcgcga
     3601 cttccttcgg ggacgaccca caccgcgacg gcgtaccggc cgggcgtgtc ggacgtcgcc
     3661 gcgcgccacg ttcccgttcc cgtccacgcc tcgccggcct ccttgagcgg cacgcgctga
     3721 aggtccatca cgacgaagtc gtgcaggagt ttcttcccct tgttctctcc agccttgacg
     3781 tctcgcgaca gcgcgccgcc cagcacggct acgtacgctt ccggacgccc gtgcatgatc
     3841 ttccgcgcgc cgctcgggaa cgcgaagcga atcgcggtgg atcccgcgaa gacatccaac
     3901 gtcaacgtcc ccatctccgc gctcgaagag gggagcggct tcccgtcgaa ccagccgcgc
     3961 cactcgctcc cgttcagaac aaagccgggt gtgtacacgc ggtccgactt ccacgccgcc
     4021 gcgtaatcgc gctggcgctc gctgaattcg ggccgagcga acacgtcgac ccatcccagg
     4081 tagtcccaat agtcgacgtg gaacgccacc gggacgacgt ccttccacag gcgcgcgtcg
     4141 gtcgcgagct tggagaacca cgcctccgcg ggcgggcagc tcgagcagcc ctcggatgtg
     4201 tagagctcca agagcgatgc gcgcgcgggg ccgcccgcaa tggtctggat cggcgcgggc
     4261 ttcggcgcgg tctgcgccca tgccgccgcc gcgacgatgc ccgccagcgc ggccacgacg
     4321 gccgcgcgct tcacgttgtt acgttccata gaaactccat cgacgcgccg ggcgcgtgct
     4381 ccacgggttc tctgcttccg aaaaaaatcc gtgcgctgtg gggccccaag agcttggtgg
     4441 ttccgttcga gacttccact gcgctgcctt cacgcaatcc tacgacgtaa atcgacggat
     4501 tggatttcgt gaactcggcc agtcgctggg cgcgcgtctc cccgccatgg ttggggaggg
     4561 tggcgtcggt gtagtgcggg ttgacctgga acgggatcaa ccgcgtcgcg tcgaagcgcg
     4621 ccggctcgac gacgggcatg tcgttggtcg tcttgatggt gggacacgcg acgttggagc
     4681 ccgcgctcca ccccatatac ggtgtccccg agagcacgcg cgcgcgaatc gcccacaagg
     4741 cgccggtgtc gtacagatgc ttcaacaagt ggaacgtgtt gcctccgccc accaggaccg
     4801 cttccgccgt ttcgatctcc tgcgccgggt gcttcgcgtg gtggatcgat cgcacttcga
     4861 ttcccagcgg ggcgaatcgc tccgccacca tgcgctcata ctcgtcccat tccaccgtga
     4921 caccggcgta gggcacgaag gccgcactcc gcgtgccggc gaagaaggcg cgcatccacg
     4981 cgtcggcgtg ctccatgtag ccgccgccga agttgcgcga gttgctcaac agcagcagtc
     5041 gttgcgttgc gttgctcatt acaggtcgac ctcgaggttg gggtaactca ccgcccataa
     5101 tcccgtatac ccagtcacgg cggtctagtg taccctcccg cgcaggagga aaaaatgccc
     5161 aagagaccag gacttcccct cgcggagtcg atccggttcg ggtggcggac ggttcgggag
     5221 tacccgctgt tcttggtcgg gaccatcctg gtcgcgatcg gcgtcccctc gctcgtcgaa
     5281 tggggaggca gggtggcgct cgacgacggc gcgccccagc tcgcgatcca ggtgatcagc
     5341 atggcggtct cggccacgct gacactgggg ctcgcgaaga tctacctgcg cttccgcgac
     5401 ggcgaacgcc ccgtcttcga gaatctcttc gacggcctcg cgcatttcca caaatatctc
     5461 ggcgcgtcca tcatcgtgtt cttcgccgtc atcatggggc tcgtcctgct cgtggtgccg
     5521 ggaatcgtgt ttctcattcg cctgtggttc ctcggcttcg tcatcgtgga cacgaaagcg
     5581 ggtccgctag aagcagtcca gcagagctgg gatatttctc gcggatacac gacggacttg
     5641 ctcgttctct tcttcatact ggtgggcgtg aacctccttg ggttgatttg cctggg
//
LOCUS       DASPGK010000127         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_33157, whole genome shotgun sequence.
ACCESSION   DASPGK010000127 DASPGK010000000
VERSION     DASPGK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33157"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VEC56_03690"
     CDS             <1..849
                     /locus_tag="VEC56_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV13287.1"
                     /translation="AKLTAEIETQAQQMGTASSPEEQMGILDAIAAKLDAFRRQYPGT
                     PEALDAAFQLGAMRASTQQFEQAEPYLVEFVGKADSSQREKLTYAHYYLAETYKNTGQ
                     YDKAEGEYKLILRDYADVNRNLTNYVSTNLDGLAAERKLAIGGDPIDFDVKSIKGERL
                     SPAAYRGKVLLIDFWATWCGPCIAEMPNVKNVYAKYHGKGFEIVGISLDQSRDKLDRY
                     IENQGISWPQYFDGKWWNNDVAVRYGVKSIPTTILVDKKGKIRYKSLRGKQLETAVQE
                     LLAEKV"
     gene            complement(871..2124)
                     /locus_tag="VEC56_03695"
     CDS             complement(871..2124)
                     /locus_tag="VEC56_03695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13288.1"
                     /translation="MSDPRRSEVPRAQARVLVVSLWETMWSLGVDADVKAGVSDDEHF
                     VERFARAGYELHFLRPASRRPITAQDPRVHTHYYPNFFRPTSRLPTWIRRPLWPLVYA
                     LVVTPRVLRLARELRIDVVLGHTYYAAPTTWWCRKRLGIPGAVKLFGVMDLVHTEWPP
                     LKYAFKNFEQLSSFKYEQSAWIVLDDGTRGGEILRERGVPAEKIHFLPNGLDVEWADL
                     TIDRGPARARYALADDAPVVLFLARLVPSKRPRDFVRAAALVLKRRPACFVFAGDGFE
                     REACERAARAAGVADHVRFLGVVPHADVPVLMAAADLFVSTSNLTNRALPTCEAMLCG
                     VPVVAYDTGDTRTVVRDGDTGAVVRDGDVEALASAIARLLDDDGTRARMSANARALAR
                     ATFTSWDERVRMEMEIIEGLLRAVR"
     gene            complement(2121..>2690)
                     /locus_tag="VEC56_03700"
     CDS             complement(2121..>2690)
                     /locus_tag="VEC56_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV13289.1"
                     /translation="KSAPESPMMFLKGPNALCGDGDVVPYPRGVEQFDYEVELAFVMG
                     KRAQRVPREGAYGYVAGYAVFMDLTARDLQVREKQWFRAKSTDGSGPLGPWLTTGDEI
                     PDPHALAISLAVNGESMQSSRTDRMTFTVDYLVHYISQTATLEPGDVVATGTPAGVGV
                     YRKPPRFLQPGDRLVASIEGLGTLSCRIG"
BASE COUNT          484 a          908 c          905 g          393 t
ORIGIN      
        1 gccaagctga ccgccgagat cgaaacccag gcccagcaga tggggacggc gtcgtcgccg
       61 gaagagcaga tgggcatcct cgacgccatc gccgccaagc tggatgcgtt ccgacgccag
      121 tatccgggca cgccggaagc gttggacgcc gctttccagc tcggggccat gcgggccagc
      181 acgcagcagt tcgagcaggc cgaaccgtac ctcgtcgagt tcgtcggcaa ggccgattcg
      241 tcgcagcgtg agaagctcac gtacgcgcac tactatctcg ccgagaccta caagaacacc
      301 ggccagtacg acaaggcgga gggagaatac aagctcatcc tgcgcgacta cgccgacgtg
      361 aaccgcaacc tgaccaacta cgtctcgacc aacctcgacg gtctcgcggc cgagcgcaag
      421 ctcgccatcg gcggcgaccc gatcgatttc gacgtgaaga gcatcaaggg cgagcggctc
      481 tcgcccgccg cgtatcgcgg caaggtccta ttgatcgact tctgggccac gtggtgcggt
      541 ccgtgcatcg cggagatgcc gaacgtgaag aacgtgtacg cgaaatacca cggaaagggc
      601 ttcgagatcg tcggcatctc gctcgaccag tcgcgcgaca aactcgaccg ctacatcgag
      661 aaccagggta tcagctggcc gcagtacttc gacggcaagt ggtggaacaa cgacgtcgcg
      721 gtgcgctacg gcgtcaagtc gattccgacc accatactgg tcgataagaa ggggaagatc
      781 cgctacaagt cgctgcgcgg caagcagctc gaaacggccg tgcaggaatt gctggcggag
      841 aaggtgtaag cccgcgtcgt ccgtcgggat tcaccgaacc gcccgtagca atccctcgat
      901 aatctccatc tccattcgaa cccgttcgtc ccacgacgtg aacgtggctc gcgccagggc
      961 gcgcgcgttg gcggacatgc gtgcgcgcgt gccgtcgtcg tcgagcagtc gcgcgattgc
     1021 gctggcgagc gcctcgacgt cgccgtcgcg gaccacggca cccgtgtcgc cgtcgcgcac
     1081 caccgtgcgc gtgtcgcccg tgtcgtaggc cacgacgggg acgccgcaca gcatcgcctc
     1141 gcacgtcggc agcgcgcggt tggtgaggtt gctggtcgac acgaagaggt cggcggcggc
     1201 catgagcacg ggcacgtcgg cgtgggggac cacgccgagg aagcgcacgt ggtcggcgac
     1261 gcccgccgca cgggccgccc gctcgcacgc ctcgcgctcg aatccgtcgc ccgcgaacac
     1321 gaagcaagcc ggacggcgct tcagcaccag cgccgccgcg cggacgaagt cgcgcggccg
     1381 cttcgacggc accagtcgcg cgaggaacag caccaccggc gcatcgtcgg ccagagcgta
     1441 gcgcgcgcgc gccggccctc gatcgatcgt gagatcggcc cactcgacgt cgagaccgtt
     1501 ggggaggaag tggatcttct cggcggggac gccgcgctcg cgcagtatct cgccgccgcg
     1561 cgtgccgtcg tcgaggacga tccacgcgct ttgctcgtac ttgaacgacg atagctgctc
     1621 gaaattcttg aacgcgtact tgagcggcgg ccattcggtg tgcacgaggt ccatcacgcc
     1681 gaatagcttg accgcgcccg gaatccccag ccgtttgcgg caccaccacg tcgtgggcgc
     1741 ggcgtagtag gtatggccga ggaccacgtc gattcgcagc tcgcgcgcca gcctcagcac
     1801 ccgcggcgtt accacgagcg cgtacacgag cggccacagc ggacggcgaa tccacgtcgg
     1861 caagcgactg gtgggccgga agaaattcgg atagtaatgc gtgtgcacgc gcgggtcctg
     1921 ggcggtaatc gggcgtcgtg aagcggggcg taagaagtgc agctcgtatc cggcgcgcgc
     1981 gaaccgctcg acgaagtgct cgtcgtcgga aacgccggcc ttgacgtcgg cgtcgacccc
     2041 gagcgaccac atcgtctccc acagcgacac gaccagaacg cgcgcctggg cgcgcggcac
     2101 ctcgctgcga cgcggatcgc tcacccgatg cggcacgaga gcgtacccag gccctcgatg
     2161 ctcgcgacca gacggtcgcc gggttgcagg aagcggggcg gcttgcggta cacacccacc
     2221 ccggccggcg tcccggtcgc gaccacgtca ccgggctcga gggtcgcggt ctgcgagatg
     2281 tagtgcacga ggtagtcgac ggtgaaggtc atgcggtcgg tgcggctcga ctgcatcgac
     2341 tcgccgttga cggcgaggga gattgcgagc gcgtgcgggt ccgggatctc gtcgccggtg
     2401 gtgagccacg gccccagcgg tccgctgccg tcggtgctct tggcgcggaa ccactgcttc
     2461 tcgcgtacct ggaggtcgcg cgcggtcaga tccatgaaca ccgcgtagcc cgcgacgtac
     2521 ccgtaggcgc cctcgcgcgg cacgcgctgc gcgcgcttgc ccatgacgaa cgcgagctcc
     2581 acttcgtagt cgaactgctc cacaccgcgc ggataaggaa ccacgtcgcc gtcgccgcac
     2641 agcgcgttgg ggcccttcag gaacatcatc ggggactcgg gcgcggactt
//
LOCUS       DASPGK010000128         4605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_502097, whole genome shotgun sequence.
ACCESSION   DASPGK010000128 DASPGK010000000
VERSION     DASPGK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502097"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..270
                     /locus_tag="VEC56_03705"
     CDS             91..270
                     /locus_tag="VEC56_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13290.1"
                     /translation="MGLTDDQRDEAAKALRGAQEAMEYLIKGVKGVDEASTLRGRIEE
                     VELYLKRAKMALKFI"
     gene            complement(267..1043)
                     /locus_tag="VEC56_03710"
     CDS             complement(267..1043)
                     /locus_tag="VEC56_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13291.1"
                     /translation="MAAPLVLLLAAACAQQKSSTPDDVSARFHSLRDSLDANFPGEAV
                     HSLEAFVDENARYQVADSARAEMLHFRSMLDGRYHEARELAREGEFDAAEYMFRDLAL
                     LPDTEDGASAARHLEFEFYFEKAKWLLVRQRFEESEAVARELLTRDLNRFQRDQVEQI
                     LDYHANVDGAMTMSQRASAEGACRQLVVFLANLYVNDGHYPSSLSLSELEGLDPYASQ
                     TISNALASIEDYRATQDTYSLVAVSKDGHRFRIENGKIED"
     gene            1220..3178
                     /locus_tag="VEC56_03715"
     CDS             1220..3178
                     /locus_tag="VEC56_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13292.1"
                     /translation="MKTALRLLAVVLALTASPNPSLADWTVDGNVVSTADDLQMNPAM
                     ASDGGTGVFVTWQDRRDGTSFNIYVQRFDETGNAVWTANGVAVCLEANDQTDPKVVSD
                     GAGGAIVVWEDARTSTNFDVYVQRVDADGNPMWTTDGVALCALDLNQHNPTIVSDDLG
                     GAVVAWQDHRGGQPDIYAQRVDADGNALWAEDGVEVCTAARAQNTPVAVADGGEGAVV
                     CWVDKRAGLGDIYVQRIDADGNALWTTNGVALCTDLEEQSVPALVVDQLGGAYVAWAD
                     KRDGNLDIYAQYVDFDGVVQWTANGLAVCAQPGDQNNPAIVGDGLATFALGVAPPGGA
                     IIAWEDFRSGLHYDIYASHVDGGGNFAWTANGVALCTAPLNQVKPVITSDANHGAIVA
                     WQDHRPNFTTDVYARRVDQGGSPQWDADGVRLCGAADNQFDPVIAFADAGAAIVAWED
                     LRTGTYDVYAQRAAGAPTAVAILSFSAVPSVEGVVLHAAFRSDLVVEGVNVYRGGPSG
                     SLTKIGALAGVGGEMVYVDRTVEPGATYRYQIGVVDADGEFWSPIVALSVAALSAALD
                     QNRPNPFNPSTTIRFVVPEREHVSLAVYDASGRAVRTLVDAITEPGAREVVWDGRDDR
                     GVAQSSGVYFYRLSTGKRAESRKMVLLK"
     gene            3357..3923
                     /locus_tag="VEC56_03720"
     CDS             3357..3923
                     /locus_tag="VEC56_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13293.1"
                     /translation="MKTCAMVLLLVALAVPAVAANHDESINGDLSTDPGAPTLLALSV
                     GGNTVIGTTGNPAGVVDRDYITFNIPAGHMLVGLTLHALAPDNIAFASFNAGTTSFVP
                     SGATAGLFLAGIHIDGGDVATNLMPLFVSESLTGNSLPTPDLGPGDYCFLIQQTSPIT
                     QGYTLEFVLAGAVPTTDKTWGAIKALYR"
     gene            4003..>4605
                     /locus_tag="VEC56_03725"
     CDS             4003..>4605
                     /locus_tag="VEC56_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXV13294.1"
                     /translation="MSARSFRVIESDDNRVFKRLAKTLTGRGIRKHDAAIVSGAKIVA
                     DVLRASPQLAEAWISTPKHEPPPAALPAAVAWYQLSPRLFQRLDVSGTGTPLLLVETP
                     PIEAWQPADGFASGCSILVPFQDPENVGAVIRSAVAFGAAHVVLLAESAHPYHPKSLR
                     ASGGAVFLAALRQGPSLHELPDDLPVVPLSTHGADVATFGF"
BASE COUNT          800 a         1509 c         1534 g          762 t
ORIGIN      
        1 cgccacgcgc gcctgggaca cgacgccgac cagccgcacc ggcttcgcta ccgaaaaatc
       61 cacgcgtagg aacgcaaagg aggcgcgact atgggactga ccgacgacca gcgcgacgaa
      121 gccgccaagg cactacgcgg cgcgcaagag gcgatggagt atctcattaa gggtgtgaag
      181 ggcgtcgacg aggcgagcac gctccggggc cgcatcgaag aagtggagct ctacctgaag
      241 cgcgccaaga tggcgctcaa gttcatctag tcctcgatct tcccgttctc gatccggaag
      301 cgatggccat ccttgctcac ggccacgagc gagtaggtgt cctgcgtcgc gcggtagtcc
      361 tcgatggacg cgagtgcgtt cgagatggtc tgcgatgcgt aggggtcgag gccctcgagt
      421 tcggacaacg ataggctcga cgggtagtga ccgtcgttga cgtagagatt ggccaagaag
      481 acaaccaact gacggcatgc accttcggcg ctggcccgct gcgacatcgt catcgcgccg
      541 tccacgttgg cgtgatagtc caggatctgc tcgacttggt cgcgctggaa tcggttgagg
      601 tcacgcgtca agagctcgcg tgcgaccgct tcgctctcct cgaagcgctg gcgaaccaag
      661 agccacttcg ccttctcgaa atagaactcg aactcgaggt ggcgcgccgc acttgcgccg
      721 tcctcggtgt cggggagcag cgcgagatcg cgaaacatgt actccgcggc gtcgaactcg
      781 ccctcccgcg ccagctcgcg cgcctcgtgg tagcgaccat cgagcatcga acggaagtgc
      841 agcatctccg cgcgcgcgct gtcggcgacc tgatagcggg cgttctcgtc cacgaacgcc
      901 tcgaggctgt ggaccgcctc cccgggaaag ttggcgtcca agctgtcgcg caacgaatgg
      961 aagcgggccg agacatcgtc cggcgtggac gatttctgct gcgcgcacgc ggcggcgagc
     1021 aacagcacga gaggcgcggc catgcggaag aacccggtca tgcggccctg gagtgcaact
     1081 ttcgggcccg gtcgccgtac gcgatatggt atcatttttc cgcaccgagg gagcgcggct
     1141 cgatcggagc cgcaaatctg ctaagggctg caatctcgtc atgagcctcg gtcgctaccg
     1201 ccgaccagga gtagcgctca tgaaaaccgc tcttcgtctg cttgcggtcg tgttggcact
     1261 gaccgcttca cccaatccgt ccctcgccga ctggaccgtc gacggcaacg tcgtatccac
     1321 cgcggacgac ctgcaaatga accccgccat ggcctccgac ggcgggaccg gggtcttcgt
     1381 cacatggcaa gaccgtcgcg acggcacctc gttcaacatt tacgtgcagc gcttcgatga
     1441 aacgggaaac gccgtgtgga ccgcgaacgg agtcgcggtt tgtctcgagg ccaacgacca
     1501 gaccgacccc aaggtcgtct ccgacggcgc cggcggtgcc atcgtggtgt gggaggacgc
     1561 gcgcacgagc accaatttcg acgtctatgt ccagcgcgtc gacgcggacg gaaatccaat
     1621 gtggaccacc gacggggtcg ccctgtgtgc gctcgacctc aaccagcaca acccaaccat
     1681 cgtctccgat gacttgggcg gtgccgtcgt cgcgtggcag gatcaccgcg ggggacagcc
     1741 cgacatctac gcgcaacgag tcgacgccga cggcaacgcg ctctgggccg aggacggtgt
     1801 ggaggtgtgc accgccgcgc gcgcgcagaa cacaccggtc gcggtagcgg acgggggcga
     1861 aggcgcggtt gtgtgctggg tggacaagcg ggcgggactg ggcgatatct acgtccagcg
     1921 catagacgcg gacggaaacg cgctgtggac gacgaacggg gtggcgctgt gcacggacct
     1981 cgaggaacag tcggtaccag cgctcgttgt cgatcagctc ggcggcgcgt acgtcgcttg
     2041 ggccgacaag cgcgatggca atctcgacat ctatgcgcag tacgtcgatt tcgacggcgt
     2101 cgtgcagtgg acggccaacg gcctcgcggt gtgcgcgcaa ccgggcgacc agaacaatcc
     2161 cgcaatcgtg ggggacggac tcgcgacctt cgcgctggga gtcgccccgc cggggggcgc
     2221 gatcatcgcg tgggaggatt ttcgcagcgg gttacactac gacatctacg cgagtcacgt
     2281 cgacggcggc ggcaacttcg cctggaccgc caacggggtg gcgctgtgca ccgcgcccct
     2341 caaccaggtc aagcccgtga tcacgtccga tgcgaaccac ggcgcgatcg tcgcgtggca
     2401 ggatcatcgc cccaacttca cgaccgacgt gtacgcgcgg cgagtcgacc agggcgggag
     2461 cccgcaatgg gacgccgacg gcgtgcgact ctgcggcgcc gccgacaacc agttcgaccc
     2521 ggtcatcgcc ttcgcggacg cgggggccgc catcgtcgcg tgggaggacc tccgcaccgg
     2581 gacgtacgac gtctatgcac agcgcgcggc cggcgccccc accgcggtcg ccattctctc
     2641 cttctcggcc gttccgagcg tggagggcgt cgtgctacac gcggcgtttc gctccgacct
     2701 ggtcgtggag ggcgtgaacg tctatcgcgg cggaccgagc ggctcgttga cgaagatcgg
     2761 cgcgctcgcc ggcgtgggag gagagatggt gtacgtcgac cgcaccgtcg aaccgggcgc
     2821 gacctatcgc tatcagatcg gcgtcgtcga cgcggacgga gagttctggt ctccgatcgt
     2881 ggcgctctcg gttgcggcgc tttccgccgc actcgatcag aatcgtccca acccgttcaa
     2941 cccgagcacg accatacgtt tcgtggttcc cgagcgcgag cacgtgtcgc tggcggttta
     3001 cgacgcgagc ggccgcgcgg tgcggacgct cgtcgacgcg ataacggaac ccggcgcgcg
     3061 cgaggtggtg tgggacggcc gtgacgaccg cggcgtcgcg cagagctcgg gcgtctattt
     3121 ctatcgcttg agcacgggca agcgagcgga gtcgcggaag atggtccttc tcaagtgagg
     3181 gagtcgcggc tggattcgaa aagaaaaatc atgtataatc atgggcagcc aactcgacac
     3241 ggcgcgcgag ggatggggaa gcgaccgggc cgcggcgtgc gtccaagatg gcagtcctcg
     3301 agcagtaccc tacagaattg gcgtagccac gacgcctcac agcaaggaga ggtaccatga
     3361 agacctgcgc tatggttttg ctactggtcg cactggcagt ccccgcggtc gcggccaacc
     3421 atgacgagtc catcaacggt gatctgtcga ccgaccccgg cgcgcccacg ctgctcgcgc
     3481 tgtcggtcgg gggcaacacc gtcatcggaa cgaccggcaa cccagcgggc gtggtcgatc
     3541 gcgactacat cacgttcaac attccggccg ggcacatgct ggtcgggctc accctgcacg
     3601 cactcgcccc cgacaacatc gcgttcgcgt ccttcaacgc gggcacgacc agcttcgtcc
     3661 ccagcggggc gacggcgggc ttgttcctgg cgggtatcca tatcgacggc ggcgacgtgg
     3721 cgacgaacct gatgccgctt ttcgtctccg agtcgttgac gggcaactcg ctgccgactc
     3781 ccgatctcgg acccggcgac tactgctttt tgatccagca gaccagcccc atcacgcagg
     3841 gctacacgct cgagttcgtg ctcgcggggg cggtcccgac cacggacaag acgtggggtg
     3901 cgatcaaggc gttgtaccgc tagttcttcg cgaacgcctc gcgaattccg aagccccggt
     3961 ggccgagttg atatagactc ggcggccggg gcttccatcg ttatgagcgc gcgttccttc
     4021 cgtgtcatcg aaagcgacga caatcgcgtc ttcaagcggc tcgcgaagac gttgaccggc
     4081 cgcggcattc gcaagcacga cgcggccatc gtctcgggcg ccaagatcgt cgccgacgta
     4141 ctgcgcgcct cgccccagct ggcggaagcc tggatcagca ccccgaagca cgagccgccg
     4201 ccggccgcgc tcccggccgc ggtggcgtgg tatcaactct cgccgcgctt gtttcaacgt
     4261 ctcgacgttt ccggaaccgg aacgccgctg ctcttggtcg agacgccgcc catcgaggcg
     4321 tggcagcccg cggacggatt cgcgtccggg tgcagcatcc tggttccgtt tcaagacccg
     4381 gagaacgtgg gcgcggtgat ccgttccgcg gtggcgttcg gcgccgcgca cgtcgttctg
     4441 ctggcggagt ccgcgcatcc ctatcacccc aagtcgttgc gcgcgtcggg cggcgcggtg
     4501 ttcctggccg ccctgcggca gggaccttcg ctgcacgaat tgccggacga tctgcccgtc
     4561 gttccgttgt ccacgcacgg cgccgacgtg gccacgttcg ggttt
//
LOCUS       DASPGK010000129         4693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1204937, whole genome shotgun sequence.
ACCESSION   DASPGK010000129 DASPGK010000000
VERSION     DASPGK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204937"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="VEC56_03730"
     CDS             <1..1654
                     /locus_tag="VEC56_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV13295.1"
                     /translation="IYDFIARDPRTYAPKLSSSEVYGSSRFVRVGPFSLPWGRAGVRG
                     LRKSRTFWSVLTDHGVFSSVLRVPITFPVEKIDGVMVAGMCVPDLRGSQGSFTFITSD
                     PARKPDGGVTVSITADGGEVEIPGPASPVKKETLTARVSIRRAAVRDGRERFQLVSGK
                     ERVSLIEGTYTPWVRLTFRAARGVTLAGIVRFLPVTLNGSIAVYMTPIQIDPERPAMP
                     VSYPSVFSIYLAKLLGPFGTLGLLEDTGALNDGAIDEQGFLDQAYLYHDERRAMWFHT
                     LARLRSGLAVCVFDVSDRLQHMFFRYLDPSHPANAGRDLTRHAGAIEDMYRRMDALVG
                     ETVQHVDDKTALFVISDHGFKTFRRGVNLNAWLEREGLLVTRDGAAPGEYLSAIDWSR
                     TRAYALGLGGIFINRKGRERHGIVDESEVDALKRRICERLVLLEDGGARCIRRVVDVA
                     REWSGPYRTIGPDLLPGYEVGYRVSWDCAKGAVRPELFEDNTKRWSGDHCMDSEVVPG
                     VLFTNVPLSSAESRLMDLGPTVLDLFGVPVPSYMTGRSLAAR"
     gene            1651..3615
                     /locus_tag="VEC56_03735"
     CDS             1651..3615
                     /locus_tag="VEC56_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV13296.1"
                     /translation="MRNRAGRLARATLAAFVISACIARAETSVVVIGIDGMDPGLLAK
                     FIAEGRMPNFARLAAEGSFDSLETSIPPQSPVAWSNFITGMDPGGHGIFDFIHRDPAT
                     YTPVFSAADVVEPGRTLSLGDWVLPLSRGGTVLLRGGEAFWQVLDRHDVPCTIVRVPA
                     NYPPAETRARSLAGMGTPDLLGSYGTFSLYTNDEAWRGAHASGGRIYSIELLDDRAQA
                     TLVGPPNTLRKDRAPLSCPMAIDADPEHDAARITIGGAQTLLFAGEWSDWVPVAFRVA
                     GPFKKLRGMVRLYLRSVTPLQLYVSPLNIDPTAPALPISTPDDYARELAARVGRYYTQ
                     GIAEDTKALEAGVFDDAEFVAQTDTLLSERSRMLDAVLDDYEGGFLFFYVSTVDQSCH
                     ALWRNADPAHPAHPAGGEFADRFGQLYEEMDAMLGRVRDRIPADATLMVLSDHGFAPY
                     YKKVNLNSWLHQNGYLALIRSDEIGQHPLFNNVFWRRTRAYGAGINGLYVNRAGRESK
                     GIVPAGQVEELLDEISRGLLALRDPETGEQVITTVYRGREVYHGAHAADAPDLVIGYN
                     RGYRGSDDSALGMVTSDVITPNLGKWTGDHCIDHRWVPGILLMNRRAAIDDPSLLDMP
                     VTILAQFGVPAPPAMTGRNVLAETTAASSR"
     gene            3640..3837
                     /locus_tag="VEC56_03740"
     CDS             3640..3837
                     /locus_tag="VEC56_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13297.1"
                     /translation="MFGKKGIVLDPGLLERAKQAAAKAGYASVDEFVAHVVERELNRS
                     EDAGADADDIKKKLEGLGYIS"
     gene            3839..4660
                     /locus_tag="VEC56_03745"
     CDS             3839..4660
                     /locus_tag="VEC56_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13298.1"
                     /translation="MESIALLITLLFDALVLPFGRHHAAALVIVSILSGAAFALVFRA
                     TSKPDRITRARGRFQARVLEMRLYPDDVVLLTRALLGALATQIDYFRAAGKPILIVLI
                     VALPVYFQLESRFAGRPLHASERALVTATVKPGLDARAIPAELTGSDGLLVDPRSVRV
                     RATREIAWRVEAKAAAVHHLTARVYDVEYRFPVQGSSRARALGRERSAGPFWSAFVHP
                     GLPSIPDNSAIETVRVSYPEQRFHLLGVDWDWLGVFLTGTLVGAAIPAVLLRIQM"
BASE COUNT          729 a         1597 c         1549 g          818 t
ORIGIN      
        1 catctacgac ttcatcgccc gcgacccgcg cacgtatgcg cccaagctct cgtcgagcga
       61 ggtgtacggg agctcgcgct tcgtgcgcgt gggaccgttc tcgctgccgt ggggtcgggc
      121 gggcgtgcgc ggactgcgca agagccgcac gttctggagc gtgttgaccg accacggagt
      181 gttcagctcg gtgttgcgcg tgcccattac ctttccggtc gagaagatcg acggcgtcat
      241 ggtggcgggc atgtgcgtcc ccgacctgcg cgggagccag ggctcgttca ccttcattac
      301 cagcgacccg gcccgcaagc ccgacggcgg cgtcaccgtt tccatcacgg ccgacggcgg
      361 cgaggtcgag attccggggc cggcgagccc cgtcaagaag gagacgctca ccgcgcgggt
      421 ttcgatacgc cgcgccgcag tgcgcgacgg ccgtgaacgc ttccagctcg tctcgggcaa
      481 ggagcgggtt tctttgatcg aaggaaccta cacgccgtgg gttcgtctca cgtttcgcgc
      541 cgcgcgcggc gtcaccctcg cgggcatcgt gcgctttctc ccggtcacgc tcaacggctc
      601 gattgcggtg tacatgacgc ccatccagat cgaccccgag cgccccgcca tgccggtgtc
      661 gtacccgagc gtattctcga tctatctcgc caagctcctg gggccattcg gaactctggg
      721 tctgctcgaa gacacgggcg ccttgaacga cggcgccatc gacgagcaag gcttcctcga
      781 tcaagcgtat ctctaccacg acgagcggcg cgcgatgtgg ttccacacgc tcgcgcggct
      841 gcgcagcggg ctggccgtgt gcgtgttcga cgtgagcgac cgcttgcagc acatgttctt
      901 tcgctacttg gacccgtccc accccgccaa cgccgggcgc gacctcacgc gccacgcggg
      961 ggccatcgag gacatgtacc ggcgcatgga cgcgttggtg ggcgagaccg tgcagcacgt
     1021 ggacgacaag actgcgctct tcgtgatttc ggaccacggc ttcaagacct ttcggcgcgg
     1081 tgtcaatttg aacgcctggc tcgagcgcga aggactcctg gtgacgcggg acggggcggc
     1141 tccgggcgaa tacttgagtg cgatcgactg gtcgcgcacg cgcgcctacg cgctgggact
     1201 aggcggcatc ttcatcaatc gcaagggacg cgagcgacac ggtatcgtcg acgagtccga
     1261 ggtggacgcg ctcaagcggc gcatctgcga gcgcctggtg ttgctcgaag acgggggtgc
     1321 gcgctgcatc cggcgcgtgg tcgacgtcgc ccgcgaatgg agcgggcctt atcgaaccat
     1381 cggacccgac ctgctccccg gctacgaagt cggctaccgc gtgtcgtggg actgcgcgaa
     1441 gggtgcggta cgccccgagc tcttcgagga caacaccaag cgatggagcg gcgaccactg
     1501 catggattcg gaggtggtgc cgggcgtcct gttcaccaac gtgccattgt cgagcgccga
     1561 gtctcgcttg atggatctcg ggccgaccgt cctggatctc ttcggcgtac cggtaccgtc
     1621 gtacatgacg ggccgaagcc tggctgcgcg atgagaaatc gcgccggcag actcgcgcgc
     1681 gcgactctcg cggcgttcgt gatctcggcg tgcatcgcgc gcgcggagac ctccgtggtc
     1741 gtgatcggga tcgacggcat ggacccggga ctgctcgcaa agttcatcgc cgaggggcgc
     1801 atgccgaact tcgcgcgctt ggcggcggag ggatccttcg acagcctgga aacgtcgatc
     1861 cctccccaga gcccggttgc gtggtcgaat ttcatcaccg ggatggatcc cggcggacac
     1921 ggcattttcg acttcatcca ccgcgacccc gcgacttaca ccccggtctt ctccgcggcg
     1981 gacgtcgtcg aaccgggccg cacgctgtcg ctcggcgatt gggtgctgcc gctttcgcgc
     2041 ggcggcacgg tgctcttgcg cggcggcgaa gcgttctggc aggtcctcga ccgccacgac
     2101 gtcccctgca cgatcgttcg cgtgcccgcc aactacccgc ccgcggaaac gcgcgcacgg
     2161 tcgcttgcgg gcatggggac gcccgatctc ttgggctcct acgggacctt ctcgctctat
     2221 accaacgacg aggcctggcg cggcgcgcac gcgagcgggg gccgcatcta ttcgatcgag
     2281 ttgctcgacg accgcgcgca agcgaccctc gtgggtccgc ccaacacgct gcgcaaggat
     2341 cgcgcgccgc tctcgtgccc gatggcgatc gacgccgacc ccgagcacga cgccgcacgc
     2401 atcacgattg gtggcgccca gacactgctt tttgcgggcg agtggagcga ttgggtgccg
     2461 gtcgcgttcc gcgtcgcggg gccgttcaag aagttgcgcg gcatggttcg actctacctg
     2521 cgctcggtaa cgccgctgca gctctacgtg tcgccgctca acatcgaccc gaccgcaccc
     2581 gcgcttccga tctcgactcc cgacgactat gcacgagagc tcgctgcgcg cgtgggccgc
     2641 tattacaccc aagggatcgc cgaggacacc aaggcgttgg aggcgggggt gttcgacgac
     2701 gcggagttcg tcgcgcaaac cgacacgctg ctctccgagc gctcgcgtat gctcgacgcg
     2761 gtcctcgacg actacgaggg aggcttcctg ttcttctacg tctccaccgt cgaccagtcg
     2821 tgtcacgcgt tgtggcgcaa cgccgacccc gcccaccccg cgcacccggc gggcggtgaa
     2881 ttcgccgacc gcttcggcca gctgtacgag gaaatggacg ccatgctggg ccgcgtgcgc
     2941 gatcgcatcc ccgccgacgc cacgctcatg gtcctctccg accatggctt cgcgccctat
     3001 tacaagaagg tcaacctgaa ctcgtggctc catcaaaacg ggtacctcgc gctcattcgg
     3061 tcggacgaga tcgggcagca cccgctgttc aacaacgtgt tctggcgccg gacacgcgcg
     3121 tacggtgcgg gcatcaacgg gctgtacgtc aaccgggccg ggcgcgagtc gaagggcatc
     3181 gtgcccgcgg gtcaggtcga ggaactgctc gacgagatct cacgcgggct tctcgcactg
     3241 cgcgacccgg aaaccggcga gcaggtgatc acgaccgtgt accgcgggcg cgaggtctat
     3301 cacggcgcgc acgccgcaga cgcccccgac ttggtgatcg gctacaatcg cggctaccgc
     3361 ggcagcgacg actcggcgct ggggatggtc acgagcgacg tcatcacgcc caacctcggc
     3421 aagtggaccg gcgaccattg catcgaccat cgctgggtgc ccggtatcct gctcatgaat
     3481 cggcgcgccg cgatcgacga cccctcgctg ctcgacatgc cggtcaccat cctggcgcag
     3541 ttcggcgtgc ccgcgccgcc ggccatgacg ggacgcaacg tgctggcgga gacgacggcc
     3601 gcgtcgagtc gctgaaggaa cactctcaag gaggaaacga tgttcgggaa gaaaggcatc
     3661 gtgctcgacc ccggcctgct cgagcgcgcg aagcaagcgg ccgcgaaggc cggctacgcg
     3721 tcggtcgacg agttcgtggc gcacgtggtc gagcgcgagt tgaatcggag cgaagacgcc
     3781 ggcgcggacg ccgacgatat caagaagaag ctcgagggcc tgggctacat ttcctgacgt
     3841 ggaatcgatc gcgcttctaa tcacgctgct gttcgacgcg ctggtgctcc ccttcgggcg
     3901 gcatcacgcg gcggcgctcg tcatcgtgtc gattctctcc ggcgccgctt ttgcgctggt
     3961 attccgggcc acgtcgaagc cggatcgcat cacgcgcgct cgcggtcgtt tccaggcccg
     4021 cgtcctcgag atgcgtctgt atcccgacga cgtggttctc ttgacgcgtg ccctcttggg
     4081 cgcgctcgcg acccagatcg actattttcg cgccgccggc aagccgatcc tgatcgtgct
     4141 gatcgtcgca cttcctgtgt acttccaact cgagtctcgc ttcgcgggcc gccccttgca
     4201 tgcaagcgag cgagcgctcg tcaccgcaac cgtgaagccg ggactcgacg cgcgcgcaat
     4261 ccccgccgaa ctcaccgggt ccgacggtct cttggttgat ccgcgctcgg tgcgcgtgcg
     4321 ggccacgcgc gagatcgcct ggcgcgtcga ggcgaaagcg gccgcggtgc accatctcac
     4381 ggcccgggtt tacgacgtcg agtaccgttt tccagtccag ggttccagcc gcgcccgcgc
     4441 gctgggccgc gagcgaagcg ccggcccgtt ttggagcgcg ttcgttcacc cgggcctgcc
     4501 ttccatcccc gacaactcgg ccatcgaaac ggttcgcgta tcctacccgg aacagcgttt
     4561 tcacctgctg ggagtcgatt gggactggct gggggtgttc ctcaccggaa ccctggtggg
     4621 tgccgcgata ccggccgtgc tgctccggat tcagatgtag tggtgccgga ggccggagtc
     4681 gaaccggcac ggg
//
LOCUS       DASPGK010000130         7917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_502738, whole genome shotgun sequence.
ACCESSION   DASPGK010000130 DASPGK010000000
VERSION     DASPGK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502738"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VEC56_03750"
     CDS             complement(<1..275)
                     /locus_tag="VEC56_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV13299.1"
                     /translation="MKFTDLFVRRPVLAMVVNLVILIAGLQSIRSLSVRQYPRSDIAV
                     INITTAYIGANADLVRGFITTPLERVIASADGIDYIESTSTQGLSTI"
     gene            complement(276..1397)
                     /locus_tag="VEC56_03755"
     CDS             complement(276..1397)
                     /locus_tag="VEC56_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV13300.1"
                     /translation="MRKRMFITLGIVLAIVAGLGYFKYRQIKIAMSAPWSAPPEAVTT
                     VVAKEESWTASQTAIGTVVPVQGVVVSADLPGLVEEITFDSGRWVKQGDVLVRLDTSQ
                     ERAQLAAASAQSDLAKLNLERAKGLREKGVMSQAEYDRNDAEAKTAEANAAAYRAIIE
                     RKTIKAPFSGRLGIRRVNLGQYLAGGDPVVPLQALDPIYVNFSVPQQEVGLLKVGAEV
                     NVLLEGLEGVAATGKITAINSLIDPDTRNIEVQATFRNPDGRLGAGMFVQVQVVGRES
                     NSVIALPASAINFAPYGNSVFVVEDVTGPDGSTYRGVRQQFVKLGVGRGDQVAVLSGV
                     KPGEEIVSSGVFKLRPGAAVEVNNSVTPSNDPAPQPEDS"
     gene            complement(1675..2253)
                     /locus_tag="VEC56_03760"
     CDS             complement(1675..2253)
                     /locus_tag="VEC56_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXV13301.1"
                     /translation="MSKKILQLVGDFAEDYEVMVPFQALQMVGHTVHAVCPDKKAGET
                     IRTAIHDFEGDQTYSEKRGHDFALNATFATVREHDYDALVISGGRAPEYIRRNARVLE
                     IVRHFFDAKKPVAAVCHGAQVLTAAGVVKGRTVSAYPAVGPEVTLAGGEFADIPISDA
                     ITDGNLVTAPAWPAHPAWLSQFLKVLGTKVVG"
     gene            complement(2333..2710)
                     /locus_tag="VEC56_03765"
     CDS             complement(2333..2710)
                     /locus_tag="VEC56_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13302.1"
                     /translation="MPVTYSFHGSLLKIELVAQYQPDDVIKAFVAAMDDPKCPKPASL
                     LLDVRRSETLATAAVVDIRRVAEYVGPYAARVGGRVAVVAASDVHFGLSRLGSVYTEG
                     VGVEAQVFRDVESAVAWLGSPKK"
     gene            complement(2727..3113)
                     /locus_tag="VEC56_03770"
     CDS             complement(2727..3113)
                     /locus_tag="VEC56_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV13303.1"
                     /translation="MIAPLLAELGYPATPEEVARRMESLECDEGGEVFAALDDGGMAG
                     VAHVQRLPVLHSNERMAQLVLIVVAARARRTGVGKALVAACEAWAKGEGCKRMLVTSG
                     EERKEAHAFYEAMGYHHYARRFAKKL"
     gene            complement(3178..7026)
                     /locus_tag="VEC56_03775"
     CDS             complement(3178..7026)
                     /locus_tag="VEC56_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13304.1"
                     /translation="MGLRPASAQNATVGLYTDPSGSTCNFTGDAPGVVTAYVVVRPGA
                     PGVTGVQFSAPLPACFGATYMGESVPPNALVIGNSQTGVSIALLSCALQPVSVMQITY
                     QQIGGTTPCCPYPILPDPTLTEVVATDCSFQPTPIGTMTARLNSDGTCPCTGNSAPDT
                     PSNPVPTDGALAQSRTPLLSWYAADADGNLAEFDLYLGTTSPPALVAAGLTQPYYRPP
                     GLDALTQYYWRVVARDGLGLESSGPTWTFTTRATNYPPFAPTPVFPTDLAWGVQLNTD
                     LAWSASDLDADVLVYDVYFGTSSTPPLVASDLLVPSYDPGQLNYSTHYYWRVVVSDGT
                     DETSGPVWTFETRGSNTPPSAPTNEQPPNTLTGVSVMPVLSWQADDPDGDPLVYRIHF
                     GTTNPPPPAAQGLTQPTYMPGTLMANTTYYWRVWVSDGQFETPGPGWSFTTGGVNSPP
                     TAPANPAPPHNQTNVALNPVLSWTSSDPDGDLLVYRVHFGTSTPPPLVAQGLTQTLYG
                     PGTLTGSTTYYWRVVVSDGASEVIGPVWSFRTGTGDIPPGQPSVGLYANPTGSLCSFT
                     GDGPGIVTAHVILRPGAVGATGVQFAAPVPGCFGAVHLSDVAPAGMLTLGTSQTGVSV
                     AIGSCVNEPVKVLEITYFRSGGATAPCCPYPIVADPHVGSIVVADCTYELYDVPGATS
                     FFNANESCPCGVEPSPPSIPTNPQPADQAVGVSFTPTLSWFASDVDGDLEEFDVYFGT
                     TPTPPLVAAGLFPMEYTPAPLDPFTQYYWRVVARDAGDLETSGPVWTFTTRVTNQPPL
                     PPSDESPHDGQVSVPLTASLSWTGTDPEGDPLTYDVYFGTAANPPLIASDLTNSSFSP
                     GALQIETQYYWRVVAKDGTDETSGSTWMFRTLFVGDVIPDGQLTVADAECALRLGLFD
                     FSCGGSGASDRADVDCSNRVTPRDARCIHKKVVDGSCTFCGGGTAAPENAVVQPVLWA
                     TNTFRDGDELVSRLFLSGVASLEAFGFYVRHDANIAFSRALRYDVTAGFEGFHWTPSP
                     LPPFIPAAVGGYTLGGVPADIGVAFVELRFVVTSGEYGYAYVEGGFDDLGGANQVVIL
                     VDDGNPTPVLISRFEAVRSGSDVEVRWDFSSDEPVDTYTLYRRDESAALPVAIAEGPA
                     DATRSYVDRTVAPGTTYRYELLVRTRGGDEFRSETATVTTAELSLALRQNHPNPFNPQ
                     TTIPFDVPGAGTSRVRLLVMDAAGRLVRTLVDEAMPGGSHAVVWNGRDQRGGAVSSGV
                     YFYVLDVGGERRTRKMVLLK"
     gene            complement(7202..>7917)
                     /locus_tag="VEC56_03780"
     CDS             complement(7202..>7917)
                     /locus_tag="VEC56_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV13305.1"
                     /translation="DPACDGKFPSLRGDWRDLMGSFDAGPLRTVYSDSLDSRVVTMER
                     GPFCEAFRNALMTTTVQRRMPFIIHYAARGNFYPFFNVAPPDTGSVSPFAEGMYLCVT
                     CPEGTQRITSDEIETETAGTFPGRYRVDEQVGACNEWGLAPLSDEALAPVSASVPTLL
                     IAGGMDYVTPVSWAQLVSSRLSDSRVVVIDYLGHYPDGMANLECLDELIRAFLRAGTT
                     VGLDISCVETMTPPPFTID"
BASE COUNT         1370 a         2621 c         2642 g         1284 t
ORIGIN      
        1 atgatcgtgc tcaacccctg cgtgctggtc gattcgatgt agtcgattcc gtccgcgcta
       61 gcgatcacgc gctcgagcgg ggtcgtgatg aagccgcgga ccaggtcggc gttggcgccg
      121 atgtaggcgg tcgtgatgtt gatcaccgcg atgtcgctgc gcgggtactg gcgcaccgag
      181 agcgagcgaa tcgactgcaa cccggcgatc aggatcacca ggttgacgac catcgccagg
      241 accggccgtc gaacgaacag gtcggtgaac ttcatttagc tgtcctcggg ctgcggcgcc
      301 ggatcgttgg acggtgtcac cgagttgttc acttccaccg cggcgccggg gcggagcttg
      361 aacacgcccg acgacacgat ctcctcgccc ggcttgaccc cggagagcac ggccacctgg
      421 tcgccgcggc cgacaccgag cttgacgaac tgctggcgca cgccgcggta cgtcgagccg
      481 tctggccccg tcacgtcttc gaccacgaag accgagttgc cgtagggcgc gaagttgatg
      541 gcggacgccg gcagcgcgat cacactgttg gactcgcgtc cgaccacctg cacctggacg
      601 aacatgcccg cgcccagcct gccgtccggg ttgcggaacg tcgcctgcac ttcgatgttg
      661 cgcgtgtccg gatcgatgag cgagttgatc gcggtgatct tgccggtggc cgcgacccct
      721 tcgaggccct cgagcaacac gttgacctcc gcgccgacct tcagcaatcc cacttcctgc
      781 tgcggaaccg agaaattgac gtagatcggg tcgagtgcct gcagcggcac gaccgggtcc
      841 ccgcccgcga ggtactgccc gaggttgacg cggcgaatgc cgagacggcc cgaaaagggc
      901 gccttgatgg tcttgcgctc gatgatcgcg cggtaggccg cggcgttcgc ttcggccgtc
      961 ttcgcctccg cgtcgttgcg atcgtactcg gcctgcgaca tgaccccctt ctcgcgcagg
     1021 cccttggcgc gttcgaggtt gagcttggcg aggtcgctct gcgccgaggc cgccgccagc
     1081 tgcgctcgct cttggctggt gtcgaggcga accaacacgt ctccctgctt gacccagcga
     1141 cccgagtcga acgtgatctc ttcgaccaac ccgggcaagt ccgcgctcac gaccacgccc
     1201 tggaccggga ccacggtgcc gatggcggtc tggctcgcgg tccagctttc ttctttggcg
     1261 acgacggtcg tcaccgcctc cggcggtgcg gaccacggcg cgctcatcgc gatcttgatc
     1321 tggcggtact tgaaatagcc gagcccggcg acgatcgcga ggacgatccc cagcgtgatg
     1381 aacattcgct tacgcatgtc gagaagctcc taaaagatgc atagacgact ccgcccggcg
     1441 gagccggata tcagttcgag atggttgggc ggcccgcgat agcgggcacc cggcttactc
     1501 ccggtggccc gacgaaccgc tggattcaag gcggtgcgga ctgcggctca atgaagataa
     1561 atcagagaat gtattcccgg ggggaaattt ttcgagaaag tcccgccccc ggcacacccc
     1621 gcccggcggc gatgcgtaga tttgccaagg aattacgccg gcgggacgct cgctctagcc
     1681 cacgactttg gtccccagca ctttcaagaa ttgcgagagc cacgccgggt gcgccggcca
     1741 cgcgggtgcg gtgaccagat tgccgtcggt gatggcgtcg ctgatgggga tgtccgcgaa
     1801 ttcgccgccc gccagggtca cctcggggcc gacggccggg tacgccgaca ccgtgcgccc
     1861 cttgaccacc ccggccgcgg tcaatacttg tgcgccgtgg cacaccgccg ccaccggctt
     1921 cttcgcatcg aagaagtggc gcacgatctc cagcacgcgc gcgtttctgc ggatgtactc
     1981 gggcgcccgc ccgcccgaga tcacgagcgc gtcgtagtcg tgctcgcgga ccgtcgcgaa
     2041 ggtcgcgttc agcgcgaagt cgtgcccgcg cttctcgctg tacgtttgat cgccttcgaa
     2101 atcgtggatc gcggtgcgaa tcgtctcgcc cgccttcttg tcggggcaga cggcgtgcac
     2161 ggtgtgcccc accatttgca gcgcctggaa cgggaccatc acttcgtagt cttcggcgaa
     2221 gtcgccgacc agctgcagaa ttttcttcga catgggtagg cctcctggag tgagatcgcg
     2281 gcgcggccga acgaattcta cgcccgagaa ccgggcgggg caagtcgcgg cactattttt
     2341 tcggcgagcc gagccaggcg accgcgctct cgacgtcgcg aaagacctgt gcttccaccc
     2401 cgacgccttc ggtgtacacc gatcccagcc ggctcaaacc gaagtggacg tcgctcgccg
     2461 ccaccaccgc cacgcgaccg cccacgcgcg ccgcgtacgg tccgacgtac tcggcgacgc
     2521 ggcgaatgtc gacgacggcc gcggtcgcga gcgtttcgga tcgtcgaacg tcgagcagga
     2581 gggacgcggg cttggggcac ttcgggtcgt ccatcgcggc gacgaacgcc ttgatcacat
     2641 cgtcgggttg gtactgagcg accagctcta tcttgagaag gctaccgtgg aacgagtacg
     2701 tcaccggcat gggggctcac ctcacgttag agcttcttgg cgaagcggcg ggcataatga
     2761 tgatacccca tcgcttcgta gaacgcgtgg gcttctttgc gttcctcgcc gctggtgacc
     2821 agcattcgtt tgcacccctc tcccttggcc cacgcctcgc aggccgccac caacgccttc
     2881 cccacacccg tgcgccgcgc gcgcgcggcg accacgatga ggaccaattg ggccatgcgc
     2941 tcgttggagt ggagcacggg gagacgctgg acgtgggcca cgccggccat gccgccgtcg
     3001 tcgagggcgg cgaacacctc gcctccctcg tcgcactcga gcgactccat tcggcgcgcg
     3061 acctcttcgg gggtggctgg atagcccaac tcggcgagca gcggggcgat caccggggca
     3121 tcggcagggg tggcggtgcg aacgtcggtg ggcacgccgc gacgatatca gacggcgcta
     3181 cttgaggaga accatcttgc gggtgcggcg ctcgccgccc acgtcgagca cgtagaagta
     3241 caccccgctc gagaccgctc ccccgcgctg gtcgcgcccg ttccacacca ccgcgtgcga
     3301 gccgcccggc atcgcttcgt cgaccaaggt ccgcaccagg cgcccggccg cgtccatcac
     3361 caacaggcgc acgcggctcg tccccgcccc cggaacgtcg aacgggatcg tcgtctgcgg
     3421 attgaacggg ttgggatggt tctgtcgcag cgccagcgag agctcggccg tggtcaccgt
     3481 cgcggtctcc gagcggaact cgtcgccgcc gcgcgtgcgc acgaggagct cgtagcggta
     3541 cgtcgtcccc ggcgccaccg tgcgatccac gtacgagcgc gtggcgtcgg ccgggccctc
     3601 ggcgatcgcg accgggagcg ccgcgctctc gtcgcggcga tagagcgtgt acgtgtccac
     3661 cggctcgtcg ctcgagaagt cccagcgcac ttcgacatcg gagcccgagc gcacggcttc
     3721 gaagcgcgag atcaggaccg gcgtggggtt gccgtcatcg actagaatca cgacttggtt
     3781 cgcgccgccg agatcgtcga agccgccttc gacgtacgcg tagccgtact cgccgctggt
     3841 cacaacgaaa cgcaactcga cgaaggcgac cccgatgtcc gcgggcacac cgccgagcgt
     3901 gtacccgccc accgccgcgg gaatgaacgg cggaagcggc gacggggtcc agtggaaccc
     3961 ctcgaagccc gcggtcacgt cgtatcgtag cgcccgcgag aaggcgatgt tggcatcgtg
     4021 tcgaacgtag aagccgaatg cctcgagcga cgcaacgccc gataagaaaa gccgcgatac
     4081 caactcgtcg ccgtcgcgga acgtgttggt ggcccacaag accggctgga cgacggcgtt
     4141 ctccggcgcc gccgtgccgc cgccgcagaa cgtgcacgat ccatcgacga ctttcttatg
     4201 gatgcagcgc gcgtcccgcg gtgttacgcg gttgctgcaa tccacgtcgg cgcggtcgga
     4261 cgcgccgctc ccgccgcagc tgaagtcaaa cagccccagt cgcagcgcgc actcggcgtc
     4321 cgcgaccgtc agctgcccgt ccgggatgac gtcaccgacg aagagggtcc ggaacatcca
     4381 cgtcgagccc gacgtctcgt cggtcccgtc cttggcaacc acgcgccagt agtactgcgt
     4441 ctcgatttgc agcgcgccgg gcgaaaagga cgagttggtg agatcgctgg cgatcaacgg
     4501 aggattcgcc gccgtgccga agtacacgtc gtacgtcaag gggtcgccct ccggatcggt
     4561 gcccgtccaa gacaacgacg cggtcagcgg gaccgagact tgtccgtcgt gcggactctc
     4621 gtcggacggc ggaagcggcg gctggttcgt cacacgcgtc gtgaaggtcc agaccggacc
     4681 ggatgtctcg aggtcacccg cgtcgcgcgc caccacacgc cagtagtatt gggtaaaggg
     4741 atccaatggt gccggcgtgt actccatggg aaacagaccc gctgcgacca gcggcggcgt
     4801 cggcgtggtc ccgaagtaca cgtcgaactc ctcgagatcg ccgtcgacgt cggacgcgaa
     4861 ccagctcaag gtcggcgtga atgaaacgcc gaccgcttgg tccgccggct gcggattggt
     4921 cggaatcgag ggcgggctag gctccacgcc gcacgggcac gactcgttcg cgttgaagaa
     4981 gctcgtcgcc cccggcacgt cgtacagctc gtacgtgcaa tcggcgacga caatcgaccc
     5041 cacgtgcgga tccgcgacga tcgggtacgg gcagcagggg gcggtggccc cgccggagcg
     5101 gaaatacgtg atctcgagca ccttgaccgg ctcgttgacg cagcttccga tcgcgaccga
     5161 gacccccgtt tgtgaggtac ccaacgtgag cataccggcg ggagcgacgt cgctgaggtg
     5221 aactgcaccg aaacaccccg gcaccggggc cgcgaactga acgccggtcg caccgaccgc
     5281 gcccggccgc agaatgacgt gggcggtcac gatgccgggc ccgtctccgg tgaagctgca
     5341 cagcgaaccg gtcgggttgg cgtacagacc aaccgacggc tgtcccggcg gaatgtcgcc
     5401 ggtgccggtc cggaaggacc acacgggtcc gatgacctcg ctggcaccat cactgacgac
     5461 gacgcgccaa taataggtgg tgctgcccgt gagcgtcccg gggccatata acgtctgcgt
     5521 caatccctgc gcgaccagcg gcggcggcgt tgacgtgccg aaatggaccc ggtacacgag
     5581 cagatctccg tcgggatcgc tgctcgtcca cgagagcacc gggttcagcg cgacgttcgt
     5641 ctgattgtgc ggcggagccg ggttggcagg tgccgtcggc ggcgagttga cccctcccgt
     5701 ggtgaagctc cagccgggac cgggcgtctc gaactgcccg tcgctcaccc agacgcgcca
     5761 atagtaggtc gtgttggcca tcaacgtccc cggcatgtac gtcggctgtg tcaaaccttg
     5821 cgccgccggc ggcggaggat tcgtcgtgcc gaaatggatt cgatagacga gcgggtcgcc
     5881 gtcggggtca tcggcttgcc acgaaaggac gggcatgacc gaaacgccgg tcagtgtatt
     5941 cgggggttgc tcgttggtcg gagccgacgg gggcgtgttc gatccccggg tctcgaaggt
     6001 ccacacgggc ccggaggttt cgtcggttcc gtcgctcacg accactcgcc aataatagtg
     6061 cgtcgaatag ttcaactggc cggggtcgta gctcggcacc aagagatccg aagcgaccag
     6121 cgggggcgtc gacgaagtgc cgaaatacac gtcgtagacg aggacgtccg cgtcgagatc
     6181 ggacgccgac cacgccaggt ccgtattcaa ctgaacgccc cacgcaagat ccgtgggaaa
     6241 caccggcgtg ggagcgaacg gcggatagtt ggtcgcgcgc gtcgtgaacg tccacgtcgg
     6301 cccagacgac tcgaggccga gcccgtcgcg agcgaccacg cgccagtagt attgcgtcag
     6361 cgcatcgagt cccggtggcc ggtagtacgg ctgcgtgagt ccggctgcca cgagcgcagg
     6421 cggcgatgtg gttcccaggt acaggtcgaa ctcggcgaga ttgccgtccg cgtccgccgc
     6481 gtaccaactc agcaacggtg tgcggctctg cgccagcgca ccgtctgtcg gtacgggatt
     6541 cgacggagta tccggcgcgc tgttgccggt gcacgggcac gtcccgtccg agttcaagcg
     6601 ggcggtcatc gtgccaatcg gcgtgggctg gaaggagcaa tcggtcgcaa ccacctcggt
     6661 cagcgtgggg tcggggagaa tcggatacgg gcagcagggc gtggtgccgc cgatctgctg
     6721 gtacgtgatc tgcatcacgg agaccggctg caacgcgcac gacagcagcg caatcgacac
     6781 gccggtctgc gagttcccga tcacgagcgc gttgggcggg accgattcgc ccatgtaggt
     6841 cgcgccgaag caggcgggca gcggagcgga gaactgaacg cccgtcactc ccggcgcccc
     6901 cggccggact acgacgtagg ccgtgacgac gcccggagca tcgccggtga aattgcaggt
     6961 ggagccggag gggtcggtgt agagcccgac cgtcgcattc tgcgccgagg ccggacgcag
     7021 gcccacgcaa gcgatgagga tcgcggcgat tactcctgta tccgggagtc gggaccgacc
     7081 gcccgggaca cgccctcgcc actgtcgcat gggcatcgga cgccgggttg gaactgtggc
     7141 gtcagagagg agtgtacacc cgtttacgca cgaacgtcaa tgcagagatt atgtaaagcc
     7201 ctcaatctat agtgaacggc ggcggcgtca tggtctcgac gcacgagata tcgagaccca
     7261 cggtggttcc cgcgcgcaaa aacgcgcgaa tcaactcgtc caggcactcc aggttggcca
     7321 tgccgtcggg atagtgtccg agatagtcga tcacgaccac gcggctgtcg ctcaatcgcg
     7381 aggagaccag ctgcgcccac gaaaccgggg taacgtagtc catgcccccc gcgatcaaca
     7441 gagtcggaac cgacgccgaa accggtgcca gcgcttcgtc ggacagcggt gcgagtcccc
     7501 actcgttgca cgcgccgact tgctcgtcga cgcggtagcg ccccgggaag gtcccggcgg
     7561 tttcggtttc gatttcgtcg ctcgtaatgc gttgggtgcc ttcgggacac gtgacacaca
     7621 ggtacatccc ctccgcgaac ggcgagacac tgcccgtgtc gggcggggcg acattgaaaa
     7681 acgggtagaa gtttccgcgc gccgcgtagt ggatgatgaa gggcattcgg cgctgcaccg
     7741 tcgtggtcat gagagcgttt cgaaacgctt cgcagaacgg gccgcgctcc atggtgacca
     7801 cgcgcgaatc gagcgagtcg ctgtagaccg tgcgcaatgg ccccgcgtcg aacgacccca
     7861 tgagatcgcg ccagtcgccg cgcagcgacg ggaacttccc gtcgcaggcg gggtccg
//
LOCUS       DASPGK010000131         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_34171, whole genome shotgun sequence.
ACCESSION   DASPGK010000131 DASPGK010000000
VERSION     DASPGK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34171"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VEC56_03785"
     CDS             <1..445
                     /locus_tag="VEC56_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13306.1"
                     /translation="GVNEKIEGFFDVCLRRGLTGRQGVMIPALNADELMLRRDVVDAV
                     KKGRFHIYAVRTVDEGIEVLTGKRAGQWIPRHGFEHNSMHDLVDRGLRHFHDRLRDAE
                     DGSTDAAVEAAVKKAKRSKGSGTPKKPPKKPPRRPPRRRRNKRGE"
     gene            451..1410
                     /gene="queG"
                     /locus_tag="VEC56_03790"
     CDS             451..1410
                     /gene="queG"
                     /locus_tag="VEC56_03790"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXV13307.1"
                     /translation="MCGHSMELPREEIVKEIARDVGFSLVGIASLESDVHANDAFARW
                     LGAGMHGDMTYLERHRVLREDATLLLPGARSAVCVALNYYQDVERKQLEMDGRDGRGR
                     FSVYVHGEDYHAVMERMLSELEARLKLAFPNAATRVCCDIQPISDRSMALRAGIAWLG
                     KNTNVISPEYGSWIFLGEVLTDLEMQADEPLETLCGKCTRCIDACPTGALDTPFVLDA
                     RRCISYLTIEKRGEIDPELASKIGIDVYGCDTCQSVCPFNRVASESLVFFRGDRSPLV
                     EMTLDELATISDEEFREKTRGSAIRRAKAEGMRRNAGLARVTR"
     gene            complement(1416..3029)
                     /locus_tag="VEC56_03795"
     CDS             complement(1416..3029)
                     /locus_tag="VEC56_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13308.1"
                     /translation="MKLLRSLALTLLLAGGLAAVGDDPAESAGPALTASRDLTDAEVR
                     QVLHRVFNAQIRAALTWTDSLAPIAKSEPKYHLMRARLQRELIPVDDEQKEVVKQLAQ
                     PLYVELDQVIATCDARIKAGETDPELRLYRGWAWMFSSHVHTYEKSFWSAGRDAKKGK
                     EDLEWYLQQKPGEPVASSLMGAFLYFADTLPSAYKFVSKLLFLPSGDRDRGLQMMELA
                     QGYDSIQETDNALILYSVYIGFEGRFEEGLEGFSFLRRVYPRHATFVRPEAILYPLMP
                     RRGSALGDSLDAAVARLSQIPANETDHTTFTLIRFQRAYADRIYQPSRAIPRLEQMIR
                     ENPEHPDWVPAFSAFELGRIMAQHGENERAKELWKRVLADTRTKYLHDEAKKMLSDLD
                     KPPAATGPGPSDVEAIYGSDIASRERVRAALEAVKNPTVADQFYLGEAWLMSGDQEKA
                     LDAYTGAINPKVCVWDHVYQMIASTRAGEILASRGEYKAAAKHYERAGSFWHKELYFD
                     WLFEARKRYFERLDEGKETTPPTMLTTSR"
     gene            3177..5009
                     /locus_tag="VEC56_03800"
     CDS             3177..5009
                     /locus_tag="VEC56_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13309.1"
                     /translation="MKDTKPNTTVETQAPPEHVAAAEQVLKALTKLVAGRKIYAANNP
                     RLEQFRGDLGAAMRKFFEFEDELVLDVDQFVIRWSDSIVYENPRREESLAFLLYKDGV
                     GELTFQPRSVGEEVDRLVEMMADELHSLGGDEDIVTRLWNANFEHIHYRVLDDYVAEE
                     FGAGSADGSSEARSDETSDHAELLPDHPSFAEKGRVIVDQAASLGSIDTYLRGIAGQH
                     HHKDDDGEREAAYQRTLRSCFNMTGDETALYRAELEREKTEDGVAAFAEAILVFTLLV
                     DASAVRDVTTVVERLVEYALHEKNPATLERVVRLVKEFRAREDLTDPVRQFCDKELAR
                     LADPDLVASFLESTSADGRADAGIAYAMVVGTPALDALVKTLHRAEGPVLHKRICDAI
                     LAIAGESPQDVLDIIDHLDVDHPEEAIDAVYLAKALKVKALTPRLRELVFYPDARVKL
                     EMLGIVAADDDADATQLLLQSLNDLDKRVRIRVLEALAARNEPKVREKLAEMAFAKDL
                     AERASDEQETIFKTLGSVGDTHTVDQLRSLLEKRKLIHLGKGNESKLLSIKALERIRD
                     RSALDVLTRLCEDSSEAVRLRAQRAKENLAAVLAGKTPKGGEDA"
     gene            5006..>5372
                     /locus_tag="VEC56_03805"
     CDS             5006..>5372
                     /locus_tag="VEC56_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13310.1"
                     /translation="MMDDRIHMGAGEATMTADDDGRTPEDRAFLKLNGEALVIRLFAA
                     SKTLRLYDLNNRATQRVLAEVMESLARIMEREGRIFIRSSNDFLLLNEYRIPVEPQHY
                     GPFEFWVGEMKKREVEAIEI"
BASE COUNT          967 a         1761 c         1771 g          873 t
ORIGIN      
        1 cggcgtcaac gagaagatcg agggcttctt cgacgtgtgc ctgcgacgcg gcctgaccgg
       61 acgccagggt gtgatgattc ccgcgctcaa cgccgacgag ttgatgctgc ggcgtgacgt
      121 cgtggacgcg gtcaagaagg ggcgtttcca catttacgcg gtacgcaccg tcgacgaggg
      181 catcgaagta ctgaccggca agcgcgcggg gcagtggatc ccgcgccacg gcttcgagca
      241 caactcgatg cacgacctgg tcgaccgcgg cctgcgccac ttccacgacc gcctgcgcga
      301 cgccgaggac ggttccaccg acgcggccgt cgaagccgcg gtgaagaagg ccaagcgcag
      361 caaagggtcc ggcaccccca agaaacctcc caagaagccg ccccgcagac cgccgcgccg
      421 ccggcgaaac aagagggggg agtagtcgcg atgtgcggtc actcgatgga gctcccgcgc
      481 gaggagatcg tcaaggagat cgcgcgcgac gtcggcttct cgctggtggg tattgcgtcg
      541 ctggaatccg atgtgcacgc caacgacgcg ttcgcgcgct ggctcggcgc cggcatgcac
      601 ggcgacatga cttacttgga gcgccatcgc gtgctgcgcg aggacgcgac cttgctcctg
      661 cccggcgcgc gctccgcggt gtgcgtcgcg ttgaactact accaagacgt cgaacgcaag
      721 cagctcgaaa tggacggccg cgacgggcgc gggcgcttct cggtctacgt gcacggcgag
      781 gactaccacg ccgtgatgga gcggatgctc tccgagctcg aagccaggct caagctcgcg
      841 ttcccgaacg ccgcgacgcg cgtctgctgc gacatccagc ccatatcgga ccgctcgatg
      901 gcgctgcgcg cggggatcgc gtggctgggg aagaacacca acgtcatctc gcccgagtac
      961 ggctcgtgga tcttcctcgg cgaagtgctc accgatctgg agatgcaggc cgacgagcca
     1021 ctcgagacgc tttgcggaaa gtgcacgcgc tgcatcgatg cctgcccgac cggcgcgctc
     1081 gataccccct ttgtcctcga cgcccgccgc tgcatctcgt acctcaccat cgagaagcgg
     1141 ggcgagatcg accccgagct ggcctccaag atcggcatcg acgtctacgg atgcgacacc
     1201 tgccagagcg tgtgcccctt caaccgggtg gcgtcggagt cgctcgtgtt cttccgtggg
     1261 gaccgcagcc cgctggtcga gatgacgctc gacgaactcg cgaccatcag cgacgaggag
     1321 tttcgcgaga agacccgcgg gagtgcgatt cggcgcgcaa aggcggaagg gatgcggagg
     1381 aacgcgggcc tcgcgcgagt gactcggtag ctaactcacc gactcgtcgt cagcatggtg
     1441 ggcggcgtgg tctccttgcc ctcgtccagc cgctcgaagt agcgcttgcg cgcctcgaac
     1501 agccagtcga agtagagctc cttgtgccag aaactccccg cgcgctcgta gtgcttggcg
     1561 gccgccttgt attcgccgcg gctggcgagg atctcgcccg cgcgcgtgct cgcgatcatc
     1621 tggtagacgt ggtcccacac gcacaccttg ggattgatgg ccccggtgta cgcgtcgagc
     1681 gccttctctt ggtcgcccga catgagccac gcctctccca gataaaactg atccgcgacc
     1741 gtcgggttct tcaccgcttc caacgcggcg cgcacgcgct cgcgtgaggc gatgtcggac
     1801 ccgtagatcg cctcgacgtc ggacgggccc ggacccgtgg ctgcaggcgg cttgtcgagg
     1861 tcggacagca ttttcttcgc ctcgtcgtgc agatacttcg tacgcgtgtc ggcgagcacg
     1921 cgcttccaca gctccttcgc acgctcgttc tcgccgtgct gcgccatgat gcggcccagc
     1981 tcgaaggcgc tgaaggcggg cacccagtcg gggtgctccg gattctcccg aatcatttgc
     2041 tccagccgag gaatcgcgcg cgacggttgg tagatgcgat cggcgtaagc gcgctgaaaa
     2101 cgaatcagcg tgaaggtggt gtggtcggtc tcgttggccg gaatttgact taggcgtgcg
     2161 accgccgcgt cgaggctgtc gccgagtgcc gagccccgcc gcggcatcag cgggtacagg
     2221 atcgcctcgg ggcgcacgaa ggtggcatgg cgcgggtaca cgcgccgcag aaacgagaat
     2281 ccttcgagcc cctcttcgaa gcgaccctcg aagccgatgt acaccgagta cagaatcagc
     2341 gcgttgtcgg tctcttgaat cgaatcgtag ccctgcgcga gctccatcat ttgcaggccg
     2401 cggtcacggt cgcccgaggg gagaaagagg agctttgaga cgaacttgta cgccgagggc
     2461 agcgtgtcgg cgaagtagag aaacgccccc atcaggctgg acgccaccgg ctcgccgggc
     2521 ttctgctgca ggtaccactc gagatcttcc ttgcctttct tggcgtcgcg acccgcggac
     2581 cagaagctct tctcgtaagt gtgcacgtgc gaggagaaca tccacgccca gccgcgatac
     2641 aagcgcagct cggggtcggt ctcgcccgct ttgatgcgcg cgtcgcaggt cgcgatgacc
     2701 tggtcgagct cgacatagag cggctgcgcg agttgtttca cgacctcttt ctgctcgtcg
     2761 tcgacgggga tcaactcgcg ttggagtcgc gcgcgcatga ggtggtactt gggctcgctc
     2821 ttggcgatcg gggccaagga gtcggtccag gtcagggccg cccggatctg cgcgttgaag
     2881 acgcggtgca ggacctgtcg cacttccgcg tccgtgaggt cgcgacttgc ggtgagggcc
     2941 ggaccggcgc tttctgccgg gtcgtcgccg acggcggcca gcccgcccgc caagagtagc
     3001 gtaagtgcaa gtgaacgcaa tagtttcatg tctgaggaga ccccttccgg gccgcgaaat
     3061 atcggcggag tatgcacgaa gcggaccgct tcggctagcc ccgaccggca atgtggggtg
     3121 gcatatgggt tgctccctct cgttggattt cccctttttg ccatgtccgg attgtcatga
     3181 aagatacgaa gccaaacacc accgtcgaga ctcaggctcc gcccgagcac gtcgccgccg
     3241 ccgagcaggt cttgaaggcg ctgaccaagc tggtcgccgg ccggaagatc tacgccgcca
     3301 acaacccgcg gctggagcag ttccgcggcg acttgggcgc tgccatgcgc aagttcttcg
     3361 agttcgagga cgagctcgtg ctcgatgtcg accaattcgt gattcgctgg agcgacagca
     3421 tcgtctacga gaacccgcgc cgcgaagaga gccttgcctt cctgctctac aaagacggcg
     3481 tgggcgagct gacgttccag ccccggtcgg tgggcgagga agtcgatcgg ctggtcgaaa
     3541 tgatggccga cgagctgcac agcttgggcg gcgacgagga catcgtcacg cggctgtgga
     3601 acgccaactt cgagcacatc cactaccgcg ttctcgacga ctacgtcgcg gaggaattcg
     3661 gggccggctc cgccgacgga tcgtcggagg cgcgctccga cgagaccagc gaccacgccg
     3721 aactcctgcc cgaccacccg agcttcgccg agaaggggcg cgtgatcgtc gaccaagccg
     3781 cgtcgctggg gtcgatcgat acgtatttgc gcggcatcgc ggggcagcac caccacaagg
     3841 acgacgacgg cgagcgcgaa gccgcgtatc agcgcaccct gcgctcgtgc ttcaacatga
     3901 cgggcgacga gaccgcgctc taccgcgccg aattggagcg cgagaagacc gaggatggtg
     3961 tggccgcctt cgccgaagcg atcctggtct tcacgctcct ggtcgacgca tccgcggtcc
     4021 gcgacgtcac gacggtggtc gagcgcctgg tcgagtacgc gttacacgaa aagaatcccg
     4081 cgacgctcga gcgcgtcgtg cgcttggtca aagagttccg cgcccgcgaa gacctgaccg
     4141 acccggtgcg ccagttctgc gacaaggagc tcgcgcgcct ggccgacccc gacctggtgg
     4201 cgtcgttcct cgagagcacc agcgccgacg ggcgcgcgga cgcgggcatc gcctacgcga
     4261 tggtggtcgg tacgccggcg ctggacgcgc tcgtaaagac cctccatcgc gcggagggcc
     4321 cggtgctgca caagcgcatc tgcgacgcca tcctcgccat tgcgggcgaa tcgccgcaag
     4381 acgtcctcga catcatcgac cacctcgacg tcgaccatcc ggaagaagca atcgacgccg
     4441 tctacctcgc caaggcgctc aaggtgaagg cgctcacccc gcgcctgcgc gagctggtgt
     4501 tctatcccga cgcgcgcgtc aagctcgaga tgctcggcat cgtggccgcg gacgacgacg
     4561 cggacgcgac ccagctcctg ctgcagtcgc tcaacgacct cgacaagcgc gtacgcatcc
     4621 gcgtgctcga agcgctggcc gcgcgcaacg agcccaaggt gcgcgagaaa ctggccgaga
     4681 tggccttcgc caaggatctg gccgagcgcg cgagcgacga gcaagagacg atcttcaaga
     4741 cgctgggctc ggtgggcgat acgcacaccg tcgaccaact gcgatcgctg ctcgagaagc
     4801 gcaagctgat ccatctcgga aagggcaacg aatccaagct gctttccatc aaggcgctcg
     4861 agcgcatccg cgaccgcagc gcgctcgacg tgctcacgcg cctgtgcgag gacagcagcg
     4921 aagcggtgcg gctgcgcgcg cagcgcgcga aggagaacct cgcggccgtg ctggcgggga
     4981 agactccgaa gggcggtgag gacgcatgat ggacgaccgc atccacatgg gagccggcga
     5041 ggccacgatg accgccgacg acgacggtcg cacgcccgaa gatcgcgcgt tcttgaagct
     5101 caacggcgaa gccctcgtca ttcgcttgtt cgcagccagt aagacgctgc gcctctacga
     5161 cctcaacaac cgcgccaccc agcgcgtgct ggccgaagtg atggaatcgc tcgcgcgcat
     5221 catggagcgc gagggccgca tcttcatccg cagctcgaac gacttcctct tgctgaacga
     5281 ataccgcatc ccggtcgagc ctcagcacta cgggcccttc gagttctggg tcggcgagat
     5341 gaagaagcgg gaagtggaag cgatcgagat cg
//
LOCUS       DASPGK010000132         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_503156, whole genome shotgun sequence.
ACCESSION   DASPGK010000132 DASPGK010000000
VERSION     DASPGK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503156"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1058)
                     /gene="aroF"
                     /locus_tag="VEC56_03810"
     CDS             complement(33..1058)
                     /gene="aroF"
                     /locus_tag="VEC56_03810"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXV13311.1"
                     /translation="MIVVMKSDATMSDISSVIKSLESMGYKPHPTRGADRTIIGVVDA
                     RQGADFSQIESTPGVEQVIPITKAYKMVSREFRPENTIVNVNGVAVGGPEFVVMAGPC
                     AVENRDQVLTAARAVKHAGASILRGGAFKPRTSPYSFQGLGEDGLKILLAAKRETGLP
                     VVTEVISPELVPLVSEYADVLQIGARNMQNYALLEAVGKIRKPVLLKRGMMSTVEELL
                     MAAEYILSNGNQQVILCERGIRTFENSTRNTLDISAVPVIKRNSHLPIIVDPSHAAGH
                     TEFVSALALAAVAAGADGLIVEVHPCPETAWCDGVQSLSIEKFNEMMTGIRAVAKAVG
                     RPLRDAS"
     gene            complement(1140..1934)
                     /gene="trpA"
                     /locus_tag="VEC56_03815"
     CDS             complement(1140..1934)
                     /gene="trpA"
                     /locus_tag="VEC56_03815"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXV13312.1"
                     /translation="MKALGDTTRALRSHGRKALVAYFTAGYPDDDAFDALVRGAADAG
                     ADVVEIGIPFSDPIADGPVIQAASNAALARGVTLARALESIARLSPLVDVPLVVMSYV
                     NPILRLGVDAFAREAARAGVAGVILPDVAFEESVAFRPALRTAGLAYIDLVAPTTDEA
                     RMSEIARASDGFVYVVSITGVTGAGAAARTDVEGIVARVRAATDTPAYVGFGVSTPEA
                     AAEVASASDGVIIGSRLVRLAGEGPAQAAAGRVGDFLASVRRALDG"
     gene            complement(1931..>2982)
                     /gene="trpB"
                     /locus_tag="VEC56_03820"
     CDS             complement(1931..>2982)
                     /gene="trpB"
                     /locus_tag="VEC56_03820"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXV13313.1"
                     /translation="QAELDSLLARYVGRPTPVYLASRFSASLANDARVYLKREDLNHT
                     GAHKINNCVGQALLARRMGKRRLVAETGAGQHGVATATVAALFGMECTVYMGEVDIAR
                     QSPNVRRMKLLGATVLPVASGTRTLKDATNETIRDWVTNVRTTHYIIGSTVGPHPYPT
                     IVRDFQSVIGREARAQMLETESRLPSDVVACVGGGSNALGIFSAFLSDDVRLHGVEAG
                     GSRDGDAATLSHGTPGVLHGAFSYLLQDDHGQVSPAHSIAAGLDYPGVGPEHSFLKDA
                     GRVTYQRVSDDDALAAFSSLARLEGILPALESAHALAFVRRAFAGAAGDGRVVLVNLS
                     GRGDKDADAVEGRLP"
BASE COUNT          479 a         1039 c          995 g          469 t
ORIGIN      
        1 gtcccaagcc ggcggtcgcg gaccgcggcc cgctagctgg cgtcgcgaag cggtcgtccc
       61 accgctttgg cgacggcgcg aatgcccgtc atcatctcgt tgaatttctc gatcgagaga
      121 ctctggacgc cgtcgcacca cgccgtctcg gggcacgggt gcacctcgac gatgagtccg
      181 tccgctcccg ccgcgaccgc cgccagcgcc agcgcagaca cgaactccgt gtgaccggcg
      241 gcgtggctcg ggtcgacgat gatcggcagg tgcgagttgc gcttgatgac cggcaccgcg
      301 gagatgtcga gcgtgttgcg cgtcgagttc tcaaaggtcc gaatcccgcg ctcgcacaga
      361 atcacctgct ggttgccgtt agaaaggatg tactcggccg ccatcaggag ctcttccacc
      421 gtggacatca ttccgcgctt caagagcacg ggcttgcgaa tcttgcccac cgcttcgaga
      481 agcgcgtagt tctgcatgtt gcgcgccccg atctggagca cgtcggcgta ttcgctcacc
      541 aagggaacca actcgggcga gatgacctcg gtgacgacgg ggagtccggt ttcgcgcttg
      601 gccgccagca ggatcttcaa cccgtcctcg cccagtcctt ggaaactata cggcgaggta
      661 cggggcttga acgcgccgcc gcgcaggatc gaagcacccg catgcttgac cgcgcgcgcc
      721 gcggtcaaga cttggtcgcg gttctcgacc gcgcacggac ccgccatcac gacgaactcc
      781 ggtccgccca cggcgacgcc gttcacgttg acgatggtgt tttcgggccg aaactcgcgg
      841 ctcaccatct tgtaggcctt ggtgatggga atgacctgct ccacaccggg cgtgctttcg
      901 atttgactaa agtcggcgcc ctggcgcgcg tcgaccacgc cgataatggt gcggtccgcg
      961 ccgcgcgtcg ggtgcggttt gtagcccatc gattcgagcg atttgatgac gctcgagatg
     1021 tcggacatgg tggcgtccga tttcatgacc acgatcatgc tgcgtgtctc cgattctcga
     1081 ttgaatggcg gcggccagcg gccgccatgg tatcacgaat gcccggtttc cgtcgcgtct
     1141 caaccatcga gcgcccggcg tacgctcgcc aagaaatcgc ccacgcgccc ggccgcggcc
     1201 tgggccggcc cctcgcccgc gagccggacg aggcggctcc cgatgatgac gccgtccgag
     1261 gccgacgcga cctcggcggc cgcctccggg gtcgagacgc cgaagccgac gtaggcgggc
     1321 gtgtcggttg cggcgcgcac gcgcgcgacg atcccttcga cgtcggtccg agccgccgca
     1381 cccgcgccgg tgacacccgt gatcgacacc acgtacacga agccgtccga cgcgcgcgca
     1441 atctcgctca tacgcgcttc gtcggtggtc ggcgccacaa ggtcgatgta cgcgagtccg
     1501 gcagtgcgca gcgcgggacg aaacgccacc gactcctcga aggcgacgtc ggggaggatg
     1561 acacccgcca caccggcgcg agcggcttcg cgcgcaaacg catccacgcc gaggcgcagg
     1621 atcgggttga cgtagctcat cacgaccagg ggaacgtcga caagaggcga gagacgcgcg
     1681 atcgactcca gcgcgcgcgc aagcgtgacg ccgcgcgcga gcgccgcgtt cgacgcggcc
     1741 tggatcacgg ggccgtcggc gatcgggtcc gaaaagggaa taccgatctc gaccacgtcg
     1801 gcgccggcat cggccgcgcc gcgcaccaac gcgtcgaacg cgtcgtcatc ggggtagccg
     1861 gcggtgaagt acgccaccag cgccttgcgc ccgtgcgatc gcagcgcgcg cgtggtatcg
     1921 ccgagcgcct tcacggcagc cgtccttcca ccgcgtcggc gtccttgtcg ccgcggcccg
     1981 agagattgac gagtacgacg cgcccatccc ccgcggcgcc ggcgaacgcg cggcggacga
     2041 acgccagcgc gtgcgccgac tcgagcgcgg ggagaattcc ctccaagcgc gcgagcgacg
     2101 aaaacgccgc gagcgcgtcg tcgtcggaaa cgcgctggta ggtaacgcgt cccgcgtcct
     2161 tcaggaagct gtgctcgggg cccacccctg ggtagtcgag tccggccgcg atcgagtgcg
     2221 cggggctcac ctggccgtgg tcgtcctgca atagatagct gaacgcgccg tggagcacgc
     2281 ccggcgttcc gtgcgacaac gtcgccgcgt caccgtcgcg cgatccgccc gcttcgacgc
     2341 cgtgcagccg cacgtcgtcg ctgaggaatg cggaaaaaat gccgagcgca ttcgacccgc
     2401 cgcccacgca cgcgaccacg tcgctcggca gccggctctc cgtctcgagc atttgcgcac
     2461 gcgcctcgcg cccgatcaca ctttggaaat cgcgcacgat ggtcgggtac gggtgtggcc
     2521 ccaccgtcga gccgatgatg tagtgcgtcg tgcgcacgtt cgtcacccag tcgcggatgg
     2581 tctcgttggt ggcgtccttg agcgtgcgcg tccccgacgc gaccgggagc acggtcgcgc
     2641 ccagtagctt catgcggcgc acgttgggac tctggcgcgc gatgtcgact tcccccatgt
     2701 agaccgtgca ctccatgccg aacagcgccg cgaccgtcgc cgtcgccaca ccgtgctggc
     2761 ccgcgccggt ttcggccacc aggcggcgct tgcccatgcg ccgcgcgagc aacgcctgcc
     2821 cgacgcaatt gttgatcttg tgcgcgccgg tgtggttcag gtcctcacgc ttgaggtaga
     2881 cgcgcgcgtc gttcgcgagc gaagccgaaa aacgggacgc gaggtaaacc ggtgtcgggc
     2941 ggccgacgta gcgggcaagg agcgagtcca gctcggcttg aa
//
LOCUS       DASPGK010000133         6293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1556882, whole genome shotgun sequence.
ACCESSION   DASPGK010000133 DASPGK010000000
VERSION     DASPGK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556882"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VEC56_03825"
     CDS             complement(<1..836)
                     /locus_tag="VEC56_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV13314.1"
                     /translation="MYEAFFSLRENPFGTTPDPRYLYKSRAHREALAYLAHGIFLKKG
                     FLAVTGEVGVGKTTIVRAFVQTFHPCLEVSFVINTKVDFREMLYMLLTDFGVEIKNDS
                     KVSMLAQLNEFLIKRYAENQNPVVVIDEAQNLSPDVLEELRMLSNLETNQQKLIQIVL
                     VGQPELWDMLQRDDLRQLRQRIPGVCRMNLLSRDEVYRYIRYRLAVAGLTNGQIVFSE
                     NAIDTIFEVTGGIPRLINMLCDRILLRGYLQRSRAIESDLVVASAHELGMGASPGRED
                     RAA"
     gene            complement(837..2228)
                     /locus_tag="VEC56_03830"
     CDS             complement(837..2228)
                     /locus_tag="VEC56_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HXV13315.1"
                     /translation="MDRSASRQETSVRDFLNVVFRRKRIILSIFVLSIVFVIVLDSRK
                     PEVWESNSRVLVRRGEQASVLSQNIRTLGWEEEVASEIQVILSDDVFKRARVLFADSA
                     RARNLAADVVFNPGGARADVIGESNVFVIGYVDARKEVCQIGCDAVTLAFRDYYRERK
                     APPLLTDFFAGEIADVRNELDAWRQRRNDFLNEKKFFGADETSKFLLTKIGHMESRLS
                     SLTGDISSQELRVANLKTLSEKSGTELEKELAFAASQNGLQTGIVQNIKFELQRLNGR
                     REELSQMYTEQHPELAAVNQQIADLHTDLKQQVENAYSVERVSLNEMKAREASLSEEL
                     GAARQLLDAVPDQERELAQIDQMIRSLDEKHKLLVGRQSEAEIARAGYPEWDVSVLSA
                     AGAPYKKKTRDFVRLALAPVLSIIIGLGIAFFLESMDHSVKSRAEAEEYLNAPVLATI
                     SERAGRKAAGGSR"
     gene            complement(2236..3036)
                     /locus_tag="VEC56_03835"
     CDS             complement(2236..3036)
                     /locus_tag="VEC56_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXV13316.1"
                     /translation="MGEAFLLSMAGLLLVTAAGCGGGIKGADRSEGWYDADSVSEFVA
                     DSGERAPLVLESEYVIGVNDVLEVVFLYHSNLSTRDLVVRRDGRISMPHVGDQMAAGI
                     TPMVLDSLLTVRFSEVLRDPNLSVIVTRPAPQKVYVLGEVASPGRFEFEDDLSMIQAV
                     ALAGGVKSGGMANHAVLIRRRGLSEIVGVEVDLRAAMNGSQMTNDLRLRNYDIVMVPK
                     HPIYSAADFMQAVGNIINVPLDAVFKGWQIANLKENYQFYQNSVTTNR"
     gene            complement(3184..3879)
                     /locus_tag="VEC56_03840"
     CDS             complement(3184..3879)
                     /locus_tag="VEC56_03840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXV13317.1"
                     /translation="MRLRPGTPSRLPTPPSATVALDAAAIDVTEPFGYAFAKRAFDLV
                     VGCAILLLLVPVIPMVGLMIRMDSQGPVFYRQERVGKKGRPFKFYKFRSMRVDSDRLR
                     AQLESSNEMTGPVFKMKNDPRVTAVGQFLRRSSLDEIPQIFNVLKGDMSIVGPRPALP
                     GEVAKYEAWHRRRLDVKPGITCLWQVAGRSHVGFDEWMRLDIEYMSRRSLRTDLGIFV
                     KTIPAVIARRGAY"
     gene            4194..4403
                     /locus_tag="VEC56_03845"
     CDS             4194..4403
                     /locus_tag="VEC56_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13318.1"
                     /translation="MLRFWVPAVGVAALLVPHDAHAYLDPGTGSYVLQMIIAGLLGAA
                     FAIKMSWVRIKRFITGLFTRHDRGD"
     gene            4387..5799
                     /locus_tag="VEC56_03850"
     CDS             4387..5799
                     /locus_tag="VEC56_03850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV13319.1"
                     /translation="MTAATDRESHTVGASFRDPAGFVFESGGHLYRQINRSFAEEFDA
                     FVGAGLYERLVRDGLLVPHEERSRSEVTSPAPELAHRIIEPERIGFVSYPYEWCFSQL
                     KDAALATLSIQSIALEHGFSLRDASAFNIQFYRGRPVLIDTLSFERYREGEPWVAYRQ
                     FCQHFLAPLALAARVDARLPLLTRPFIDGIPLDLASRALPAASRLSPALLIHIHLHAR
                     SQRRFASSTAITKRRVSRLAMRGLVDSLAGAVKQQRWTPAGTEWGDYYAATNYSDAAR
                     DRKAEVIRAFIAVAAPARVWDLGANTGEFSRAASDRGIETVAFDVDPAAVEKNYRRVR
                     EKAEAHILPLVMDLTNPSPALGWDHDERASLAGRGPADLVLALALVHHLAISNNVPLA
                     RVARFFARLGRWLVVEFVPKGDSQVQRLLATRADVFPDYHRVGFEAAFGGFFETVDVR
                     AVEGSERSLYLMRRRGEPEP"
     gene            5796..>6293
                     /locus_tag="VEC56_03855"
     CDS             5796..>6293
                     /locus_tag="VEC56_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13320.1"
                     /translation="MRRWPLLLPFLFAVAPTLTLYASNSGQTAFAAALLPMGAALALT
                     LLLVPPCVVLFRSPVRAAVALSMFWWVVFSYGHVAAVIGKFPLAGHNPANAKFLLPFT
                     CLLLVAGAVAIRRARSEPVLLARVLLVVVASTLVASLVTVARVGASRRPFASSSVFPD
                     ESLAAT"
BASE COUNT         1031 a         2175 c         1920 g         1167 t
ORIGIN      
        1 gccgcgcggt cttcgcgacc gggcgacgcc cccattccca gctcgtgtgc gctcgccacc
       61 accaagtcgg actcgatcgc acggctgcgc tgcaggtagc cccgcagcag aatgcgatcg
      121 cacagcatat tgatcaaacg cggaatgccg cccgtcactt cgaaaatcgt gtcgatcgcg
      181 ttctccgaga agacaatctg gccgttggtc agacccgcga ccgccagacg gtaacggatg
      241 tagcggtaca cttcgtcgcg cgagagcagg ttcatccggc acacaccggg gatgcgctgt
      301 cgcagctgcc gcaggtcgtc gcgctggagc atgtcccaca gttccggctg ccccaccagc
      361 acgatctgga tgagcttctg ctggttggtc tccaggttcg acagcatgcg cagctcttcc
      421 agcacatccg gggagagatt ctgggcctcg tcgatcacca ccaccgggtt ctggttctcg
      481 gcgtagcgct tgatcaggaa ctcgttcaac tgcgccagca tcgagacctt ggagtcgttc
      541 ttgatctcga ctccgaagtc ggtcagcagc atgtacagca tctcgcgaaa gtcgaccttg
      601 gtattgatca cgaaagacac ttcgaggcac gggtgaaacg tctgcacgaa cgcgcgcacg
      661 atggtggtct tgcccacccc cacctcgccc gtgacggcca ggaacccctt cttgaggaag
      721 atgccgtgcg ccaggtacgc gagcgcctcg cggtgggccc ggctcttgta caggtagcgc
      781 gggtccggcg tagtcccgaa cggattctcg cgcaagctga agaacgcttc gtacatctag
      841 cggctcccgc ccgcggcctt gcgaccggcc cgctccgaga tggtcgcgag caccggcgcg
      901 ttcaagtact cctcggcctc ggcccggctc ttcaccgagt ggtccatgct ctccaggaag
      961 aacgcgatgc ccaggccgat gatgatgctc aacaccggcg ccagggccaa tcgcacgaag
     1021 tcgcgcgtct tcttcttgta gggcgcgccc gccgccgaga gaaccgaaac gtcccactcg
     1081 gggtagccgg cgcgcgcgat ctccgcttca ctctggcggc ccaccagcag cttgtgcttc
     1141 tcgtccaggc tgcggatcat ctggtcgatc tgagccagct cccgctcctg gtccggcacc
     1201 gcgtccaaca gctggcgcgc ggccccgagc tcctcggaca gggacgcctc gcgcgctttc
     1261 atctcgttca gggagacccg ttcgacgctg tacgcgttct ccacctgctg cttgagatcc
     1321 gtgtgcagat ccgcaatctg ttggttcacg gccgccagct cggggtgctg ctcggtgtac
     1381 atctgcgaga gttcttcccg ccgcccgttc aggcgctgca actcgaactt gatgttctgg
     1441 acgattccgg tctgcagccc gttctgcgac gccgcgaacg cgagctcctt ctcgagctcg
     1501 gtccccgact tttcactcag cgtcttcagg ttggcgaccc gcagttcttg cgacgagatg
     1561 tcgccggtca gcgaggagag acgcgattcc atgtgcccga tcttggtcaa cagaaacttg
     1621 ctggtctcgt ccgcgccgaa gaacttcttc tcgttcagga agtcgttgcg gcgctggcgc
     1681 cacgcatcca gctcgttgcg cacgtccgcg atctcgccgg cgaagaaatc ggtcagcaac
     1741 ggcggagcct tgcgctcgcg atagtaatcg cggaaggcca gcgtcaccgc gtcgcagccg
     1801 atttgacaca cttccttgcg ggcatcgacg tagccgatta cgaagacgtt cgattcgccg
     1861 atcacgtccg cgcgcgctcc gccgggattg aatacgacgt ccgcggccag gttgcgtgcg
     1921 cgggccgagt ctgcgaacag gacccgcgcg cgcttgaaca cgtcgtccga gagaattacc
     1981 tggatctccg acgccacttc ctcttcccac cccagcgtgc ggatgttctg cgagagcacg
     2041 ctcgcctgct cgccgcgacg caccagcacg cgcgaattcg actcccacac ctcgggcttg
     2101 cgcgaatcga ggacgatcac gaatacgatc gacagaacga agatcgacaa gatgattcgc
     2161 ttgcggcgaa acaccacgtt caagaagtcg cgtacgctcg tctcttgcct gctggcgctg
     2221 cggtccatgt tgcccctagc gattcgtcgt aaccgagttc tggtagaact ggtagttctc
     2281 tttgaggttc gcgatctgcc agcccttgaa cacggcgtcg agtggaacgt tgataatgtt
     2341 gcccaccgct tgcatgaagt cggccgcgct gtagatgggg tgcttcggca ccatcacgat
     2401 gtcgtagttg cgaagccgca aatcgttcgt catctgggat ccgttcatgg ccgcgcgcag
     2461 gtcgacttcc accccgacga tctcggatag tccgcgacgc cggatcagca cggcgtggtt
     2521 cgccatgccg ccgctcttga caccgcccgc gagcgccacc gcctggatca tcgacaggtc
     2581 gtcctcgaac tcgaagcgcc ccggactggc gacttcgccc agcacataga ccttctgcgg
     2641 cgcgggccgg gtcacgatca cggataggtt ggggtcgcgc agtacttcgc tgaagcgaac
     2701 cgtgagcaaa gagtcgagca ccatgggcgt gatccccgcc gccatttggt cccccacgtg
     2761 cggcatcgaa atgcgaccgt cgcggcgcac gaccaaatcg cgcgtgctca agttggagtg
     2821 atacaagaac acgacctcga gcacgtcgtt gacgccgatg acgtactcgg actcgagcac
     2881 gagcggcgcg cgctcacccg aatcggcgac gaactcgctc accgagtcgg cgtcgtacca
     2941 accctcggaa cgatcggccc ccttgatccc cccaccgcaa ccggccgccg tcaccagcaa
     3001 taacccagcc atcgagagca ggaacgcttc gcccacgcgt cgcccgcgcg ggcgaacccg
     3061 cccaagcctg agtcgattgg cgcaggtcgg cgcctgtccc ggtgtgtaac tcattgttga
     3121 aacagaggaa ttcgaatcga ttgacaaacg actacctcgc aaacgcaatc gccatgccac
     3181 gggttaatac gcgccacgcc gcgcgatcac ggccggaatc gtcttgacga agatcccgag
     3241 atcggtgcgc aggctgcggc ggctcatgta ctcgatatcg aggcgcatcc actcgtcgaa
     3301 gccgacgtgg ctgcgcccgg cgacctgcca cagacacgtg atacccggct tcacatccag
     3361 gcggcgccgg tgccacgcct cgtacttggc gacctcgccg ggcagcgccg gccgcgggcc
     3421 cacgatgctc atgtcgcctt tgagcacgtt gaaaatttgc ggtatctcgt ccaggctcga
     3481 gcgccgcaag aactgcccga cggccgtgac acgggggtcg ttcttcatct tgaataccgg
     3541 cccagtcatc tcgttggatg attcgagctg cgcgcgcagg cgatccgaat ccacccgcat
     3601 cgagcgaaac ttgtagaact tgaacggacg ccccttcttc ccgacgcgct cctgccgata
     3661 gaacaccggc ccttgcgagt ccatccggat catcaacccg accatcggaa tcaccggcac
     3721 cagaaggagc aaaatcgcgc aacccacgac gaggtcgaac gcgcgtttcg cgaatgcgta
     3781 tccgaacggt tcggtaacgt cgatcgccgc cgcgtcgagt gcgacggtcg cggagggcgg
     3841 ggtgggcagc cgggacggcg tgccgggtcg caagcgcaaa ggtgtatcgc cgttcatggt
     3901 cgtctgcaca cgcaatcaca ccgcgcgcgg ggcgcggtgg actcgatcgt tcgttgtcgg
     3961 atgggccgcc cttgccacga ggcgagcgcg gggccaagat gcgttagcag aggacctagt
     4021 cgaacacctc cagagagccg aatagtagca ggaaccgccg gttagcgtca aggcgcatgt
     4081 cccggccttc cccgccgcga ttgccaggcc ggtcgggaat ctgctaccat tgcggcagga
     4141 ttgctcgccg cgccggaccg cgttgggcac ggtcgtattc acggaggaaa gagatgttgc
     4201 gtttctgggt ccccgcggtg ggcgtagccg ccctgctcgt cccccacgat gctcacgcgt
     4261 acctcgaccc cggtaccggg agctacgtcc tgcagatgat catcgccggc ctcctgggcg
     4321 ccgctttcgc gatcaagatg tcgtgggtcc gtatcaagcg tttcatcacc ggtctcttca
     4381 cgcgccatga ccgcggcgac tgaccgggag agccacaccg tcggcgcatc gttccgggac
     4441 cccgcgggct tcgttttcga gagcggcggg catctctacc gccaaatcaa ccgctccttc
     4501 gccgaggaat tcgacgcctt cgtcggcgcc ggcctctatg agcggctggt gcgggacggc
     4561 ctgctcgtcc cccacgagga gcgttcccgc tccgaggtga cctcgcccgc gccggaactg
     4621 gcccaccgta tcatcgagcc ggaacgcatc ggcttcgtct cctatcctta cgagtggtgc
     4681 ttctcgcagc tcaaggacgc cgcactcgcc accctttcga tccaatcgat cgcgctggag
     4741 cacggcttct cgctgcgcga cgctagtgcc ttcaacatcc agttttaccg cggacggccg
     4801 gtcttgatcg atacgctctc gttcgaaaga taccgggagg gcgaaccgtg ggtcgcgtac
     4861 cggcagttct gccagcattt cctcgccccg ctcgcgctcg cggcgcgcgt cgatgcgcgt
     4921 ctcccgctgc tcactcgtcc cttcatcgac ggtattcccc tcgaccttgc gagccgtgcg
     4981 ctgcccgccg cctcgcgcct atcgcccgcg ctcttgatcc acatccacct ccacgcgcgt
     5041 tcgcagcgac gcttcgcgtc gtcgaccgca atcacgaagc gtcgcgtgag ccgcctcgcg
     5101 atgcgcggac tagtcgacag cctggcgggt gcggtgaagc agcagcgctg gacgccggcc
     5161 ggcacggagt ggggcgacta ctacgcggcc accaactact ccgacgccgc tcgcgatcga
     5221 aaagccgagg tcatccgcgc gttcatcgcc gtggccgccc ccgcccgggt gtgggacctg
     5281 ggcgccaaca cgggcgagtt cagccgtgcc gcctccgacc ggggaatcga gaccgtcgcc
     5341 ttcgacgtcg atccggccgc agtcgaaaag aattaccgac gcgtgcggga gaaggcggaa
     5401 gcccacatcc tccccctggt catggacctg accaatccga gccccgcgtt gggctgggac
     5461 cacgacgagc gcgcctcgct ggcgggtcgc gggccggccg acctcgtgct ggcgctcgcg
     5521 ctcgtgcacc atctcgcgat ctccaataac gtcccgctcg cgcgcgtggc gcgttttttc
     5581 gcgcggctcg gtcgctggtt ggtggtcgag ttcgtcccca agggtgactc ccaggtccag
     5641 cgcctgctcg cgacgcgcgc cgacgtgttt ccggactacc atcgcgtggg tttcgaggcc
     5701 gccttcgggg gctttttcga gaccgtcgac gtgcgcgcgg tcgagggaag cgaacgctcg
     5761 ttgtacctca tgcgccggcg gggtgagccc gagccgtgag gcgctggccg ctgctgctcc
     5821 cgttcttgtt cgcggtcgcg cccacgctca cgttgtacgc gagcaactcg gggcagaccg
     5881 cgttcgccgc ggctctcctc cccatgggcg cggcgctggc gttgacgctc cttcttgtgc
     5941 cgccgtgcgt cgtcctattt cgcagcccag tgcgcgccgc ggtcgcgtta tcgatgtttt
     6001 ggtgggtcgt gttctcgtac ggccacgtcg cagccgtgat cggcaagttt cccctcgccg
     6061 ggcacaatcc cgccaacgcc aagttcctgc tccccttcac gtgcctgcta ttggtggccg
     6121 gggcggtagc gatccgccgc gcgcgctccg agccggtctt gctcgcgcgc gtgctcttgg
     6181 tggtcgtggc ctcgacgctc gtcgcctcgc tggtcacggt ggcgcgcgtg ggcgcctcgc
     6241 gccgcccctt cgcgtcgtct tccgtctttc ccgacgagtc gttggccgcc aca
//
LOCUS       DASPGK010000134         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_503377, whole genome shotgun sequence.
ACCESSION   DASPGK010000134 DASPGK010000000
VERSION     DASPGK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503377"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VEC56_03860"
     CDS             complement(<1..542)
                     /locus_tag="VEC56_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXV13321.1"
                     /translation="MAKIDAFLRIMQEHDASDLHIGADFEPMLRIHGTLEKTSHRVLT
                     TEEVKVLVYELLTDSQIEVLERNGELDCAYTLHGVARFRINVFRKHPGLAAAFRMIPH
                     DIPTLDALGFPETLKRMLHQKNGLILVTGPTNSGKSTTLAAMVDYLNEHMNYHMITLE
                     DPLEFIHSNKNSLINQRQIG"
     gene            complement(720..1763)
                     /locus_tag="VEC56_03865"
     CDS             complement(720..1763)
                     /locus_tag="VEC56_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV13322.1"
                     /translation="MKPQFQWLVGGAEAEAGSKASNAGDPIDERELLDAYSRAVVDVV
                     DVVTPSVVSIRVVAENRRGGTGEGAGSGFVVAPDGFVVTNDHVVERAKSVAVVFTDGR
                     ELDARVVGTDPATDLALLRVPDGGLAPVVIGASEMLRVGQMAIAIGNPLGFQSTVSAG
                     VVSALGRNLRSRSGRLIENVIQTDVALNPGNSGGPLVDSRGRVIGVNTAMIREAQGLC
                     FAIPSATMTWVVGDLMTRGRVRRAVLGLVAETRPVSRRVQRGFDLPRAAAVHVVSVAE
                     NGPAHRAGLRTHDLIVTMDGRGVATVDDLQRTLAKWTPGESLAITVLRNGGHRHDLVV
                     VPSEDLPRASLAN"
     gene            1967..3148
                     /locus_tag="VEC56_03870"
     CDS             1967..3148
                     /locus_tag="VEC56_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXV13323.1"
                     /translation="MSTKILVVDDEPDLEQLIRQKFRRSIRDNELVFLFARNGVDAVA
                     TLAAEPDVELVLTDINMPEMDGLTLLGKIDELNAAALKSVVVSAYGDLPNIRVAMNRG
                     AFDFVTKPIDLGDLETTIQKALRESALIKEALRTRDELIALQRDLDVATDIQMSMLPR
                     TLPSVADREGVDIYATMLPAKEVGGDLYDFFRVDENRIGFVVGDVSGKGVPAALLMGI
                     SRTLLRSAGRSGVAPDECLATVNRVLSEEILPNMFVTVFYGVIDTLTGEMAYSSAGHN
                     PPYLLTGDGRVTALNGVGGPPIGVVGNAPYQQERVTLGPKDAVLLFTDGVTEAMDLND
                     EFFGEKRLEELLSDSHALASESLVRRTIEAVQAFSKGAPQADDITCLALRFLGRGRPL
                     E"
     gene            3171..4496
                     /locus_tag="VEC56_03875"
     CDS             3171..4496
                     /locus_tag="VEC56_03875"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HXV13324.1"
                     /translation="MAFLNRIRGWVGLFAVASVAFAGCSADRTDVSSPVGNESPVVHA
                     PLASFVSQETTINNFVVRFDGRTVSGGNTTFTYTVRGTGVDPNLSHFTVELPACAPAP
                     LSFSPAGSANVNTNPQSGIYGVEWHLSVSKDNLAGQTYSITFPGEVALGIVRSEVKTE
                     NGARGVGSVFGPCSGFRISGTVFVDADNNGLLSATEAGIEGVTVTVSDGAATQSSLTD
                     AAGGYTFVVTSGTYTVSIEASTAAADLNEDLFATATATTPTSRTVTTGPDAPNTNFAF
                     EPASGEVVDDLKTGVLLTAGKPVRFWKTQLRNAQMGKASTYSAAQLLAWLGQIEGLGI
                     ADPFQFGTGNEINEAFAILSDNTRNDIVALKRELLASELNLVSGNGLVGQEDLHEVLI
                     IWGEELVAQASAPAGPIAPAAIKEFSEAQSATGMFGLMNGSTGGGGTDE"
     gene            4540..>5796
                     /locus_tag="VEC56_03880"
     CDS             4540..>5796
                     /locus_tag="VEC56_03880"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXV13325.1"
                     /translation="MTALGFLLPGVVAGQELPFAHYTPASDRTPLPSAEVIRTYQDRD
                     GYLWFVIFSSGIVRYDGHSFETYTISDGLRDLAVWEIVEDRSGRLWVGSNAGLVVSER
                     PLGEYPPGERIRFVVSVGGVDLIDTLINRNRLVADARGRVWAGTSSTGIVRYHVASDG
                     DSIRTTCDTLTTDPHGEGKNATVRSIAVRRDGSVWASIGGGMLLEFRDDTSTVSAISQ
                     ESGLPTQSITTLYESPRGVLWGGCRNGLIWRLDETSDTRRVVTVNERLSASIAAMLET
                     SDSTLWVASDGAGVLTLDPAHPERASPITRADGLLSDNVHHVQLDREGSLWFSQSGGA
                     SKLRTNYEAFRNYTAAPRSDVPSTLPSPAVNVVIPPAATNYSALWLGTTESGVVAIRA
                     DGTVSILDVEQGLRNNWVNGLVFDGAG"
BASE COUNT          979 a         1915 c         1915 g          987 t
ORIGIN      
        1 tcgccgatct gcctctgatt gatgagcgag ttcttgttgg aatgaatgaa ctcgagggga
       61 tcttcgagcg tgatcatgtg gtagttcatg tgctcgttga gatagtcgac catggccgcc
      121 agcgtggtcg acttgcccga gttggtcggc cccgtcacca agatcaatcc gttcttctgg
      181 tgcagcatgc gtttgagcgt ctcgggaaat ccgagcgcgt cgagcgtggg aatgtcgtgc
      241 gggatcattc gaaacgccgc cgccagaccg ggatgctttc gaaacacgtt gatgcggaag
      301 cgcgcgacgc cgtgcagcgt gtaggcgcag tcgagctcgc cgttgcgctc cagcacctcg
      361 atctgcgaat ccgtgagcag ctcgtacacg agcaccttga cctcttcggt ggtgagcaca
      421 cggtggctcg tcttctcgag ggtgccgtgg atgcgcagca tgggctcgaa gtccgcgccg
      481 atatgaagat cggaagcgtc gtgctcctgc atgatgcgga ggaatgcgtc gatcttagcc
      541 atggtgctgg acttcctcct ggtgcctcgc gttcgggagc aaaataggcg acggtagacc
      601 ttcaaggggc agcaaggagt gtgcccggga ataattctcc cggccactgg aacctcctga
      661 aatttatcgg cttacgacgg ccggtgtgcc agatcagcgt cctcggctcg gccggcgtct
      721 caatttgcga ggctggctcg cggaaggtcc tcggaaggca cgacgacgag gtcgtggcga
      781 tgaccgccgt tgcgaagcac ggtgatggcg agcgactcgc cgggagtcca cttggcgagc
      841 gtgcgctgga ggtcgtcgac ggtcgcgacc ccgcgcccgt ccattgtcac gatgagatcg
      901 tgggtgcgca gaccggcgcg gtgcgcgggt ccattctcgg ccacggagac gacgtgaacg
      961 gcggccgcgc gggggaggtc gaagccgcgc tgcacgcgcc ggctgaccgg acgcgtctcg
     1021 gcgacgagcc ccagcaccgc gcgccgaacg cgcccgcgcg tcatcaagtc gcccaccacc
     1081 cacgtcatgg tcgccgacgg aatcgcgaag cacagcccct gcgcctctcg gatcatggcg
     1141 gtgttcacac cgatcacgcg gccgcggcta tcgaccagcg gaccaccgga gttgccgggg
     1201 ttgagtgcca catcggtctg aatcacgttc tcgatcaaac gccccgaccg gctgcgcaag
     1261 ttgcgcccga gcgccgacac gacgcccgcc gacaccgtgc tctggaaccc gagcgggttg
     1321 ccgatcgcga tcgccatctg ccccacgcgc agcatttccg acgcgccgat cacgaccggc
     1381 gcgaggccgc cgtcgggaac gcggagcagg gccaagtcgg tggcggggtc ggtgccgacc
     1441 acccgcgcat cgagttcgcg cccgtccgtg aacacgaccg ccacgctctt cgcgcgttct
     1501 accacgtggt cgttggtgac gacgaagccg tcgggtgcga ccacgaagcc ggagcccgcg
     1561 ccctccccgg taccgccgcg gcgattctcg gccaccacgc ggatgctcac gaccgacggc
     1621 gtcaccacgt cgacgacatc gaccacggcg cgggaatacg cgtccagcag ctcgcgctcg
     1681 tcgatcggat cgccggcgtt cgaggccttg gaacccgcct cggcttccgc cccgccgacc
     1741 agccattgga actgtggttt catagcacgt gcctccggcg gcaatatagc gacgctgccg
     1801 cggcgccgca aacgagcggc ccggggccgc ggaacggcac aaacgctggt atgcccggcc
     1861 gccgacgtgc tatcatattg ccacgcgcgc cctaagggcg ggttgcgcgt cccatccatt
     1921 tctcacggat gggtcctccc tcgacccgtt tggagtacgg acctcgatgt ccacgaagat
     1981 actcgtcgtc gacgacgagc cggatctgga gcagttgatc cgccagaaat ttcggcggag
     2041 cattcgcgac aacgagttgg tcttcctgtt cgcccgcaac ggcgtcgacg ccgtcgccac
     2101 gctggcggcc gagcccgacg tcgagctcgt gttgaccgac atcaatatgc cggagatgga
     2161 cgggctgacg ctgctgggca agatcgatga gctgaacgcg gccgcgctca agtccgtggt
     2221 cgtgtcggcg tacggcgacc ttccgaacat tcgcgtcgcc atgaatcgcg gggcgttcga
     2281 cttcgtcacc aagccgatcg acctcggtga cctggagacc accatccaga aggccttgcg
     2341 cgaatccgca ctgatcaagg aagccctccg cacgcgggac gagctgatcg cgctccaacg
     2401 cgatttggac gtcgcaaccg atattcagat gtcgatgctg ccgcgcacct tgccgtcggt
     2461 ggccgaccgg gaaggcgtgg acatttacgc gaccatgctg ccggccaagg aagtgggcgg
     2521 cgatctgtac gatttcttcc gcgtggacga gaaccgcatc ggcttcgtcg tcggcgacgt
     2581 ctccggcaag ggtgttccgg ccgcgttgct catggggatc agccgcacgc tgctccgcag
     2641 cgcagggcgg tcgggcgtcg caccggacga gtgcctggcg acggtcaatc gcgtcctgtc
     2701 cgaagagatt ctgcccaaca tgttcgtcac ggtcttctat ggcgtgatcg acacgctgac
     2761 gggcgagatg gcgtactcga gcgccggtca caacccgccc tacctgttga cgggcgatgg
     2821 acgcgtcacc gccctcaacg gggtcggcgg cccccccatc ggggtggtcg gtaacgcacc
     2881 ctatcagcag gaacgggtca cgctgggccc gaaggacgcc gtgctcctct tcacggacgg
     2941 cgtcaccgaa gcgatggact tgaacgacga attcttcggg gagaaacggc tcgaggagct
     3001 tctttccgat tcgcacgcgc ttgcctcgga gtccctggtc cgccggacca tcgaggcggt
     3061 gcaggcgttt tcgaagggcg cgccgcaggc cgacgacatt acctgtctgg ccctgcgctt
     3121 tttgggtcgc gggcgaccgt tggagtaacc tacataggag gcatggcacc atggcattct
     3181 tgaatcgcat ccgtgggtgg gtcggactgt tcgccgtcgc gagcgttgcg ttcgctggct
     3241 gctcggcgga ccggaccgac gtttcgtcgc cggtcggcaa cgagtcgccc gtcgtccacg
     3301 ctccgttggc gagcttcgtt tcgcaagaga cgacgatcaa taacttcgtg gtccgtttcg
     3361 acggccggac cgtttcggga ggaaacacga cgttcacgta taccgtgcga ggcaccggcg
     3421 tcgatccgaa cctcagccac ttcaccgtcg agctgccggc gtgcgcgccg gcgccgctgt
     3481 cgttcagccc ggccggctcg gcgaacgtca acaccaaccc gcaatcgggg atatatggcg
     3541 tcgagtggca cctctcggtt tcgaaagaca acctcgccgg gcagacctat tccatcacct
     3601 tcccgggcga ggttgcgctg ggcatcgtcc gctccgaagt gaagaccgaa aacggcgccc
     3661 ggggcgtggg cagcgtgttc ggcccgtgta gcgggttccg catctcggga acggtgttcg
     3721 tcgacgccga caacaacggc ctcctgtccg cgacggaagc cggcatcgag ggcgtcaccg
     3781 tcacggtgtc cgacggggcg gccacacagt cctcgctaac cgacgccgcc gggggttata
     3841 ccttcgtcgt gacctcgggc acctatacgg tatcgatcga ggcttccact gcggcagccg
     3901 acctgaacga ggacctcttc gccacggcga cggcgaccac gccgacgtcg cggacggtta
     3961 ccacgggtcc cgacgcgccc aacacgaact tcgcgttcga accggcgtcg ggcgaagtcg
     4021 tcgacgattt gaagaccggc gtgttgctca ccgcgggcaa gcccgtgcgc ttctggaaga
     4081 cccagttgag aaacgcgcag atgggcaagg ccagcaccta cagcgcggcg cagctcctcg
     4141 cgtggctggg ccagatcgag gggctgggca tcgccgaccc gttccagttc ggcaccggga
     4201 acgagatcaa tgaggcgttc gcgatcctca gcgacaatac gaggaacgac atcgtcgcgc
     4261 tcaagcgcga gctgctcgcc tcggagctca acctggtttc gggcaacggc ctcgtcggcc
     4321 aggaagacct gcacgaagtg ctgatcatct ggggagagga actggtcgcg caggcgtcgg
     4381 cgccggcggg tcccatcgcc cccgcggcca tcaaggagtt cagcgaagcc cagagcgcga
     4441 cgggaatgtt cgggctgatg aacggcagca cgggcggcgg tggcacggac gagtagtctc
     4501 cattccgatg cggcgggagc gcggcgccga gtcgccctct tgaccgccct ggggtttctc
     4561 ctgcccggcg tcgtcgccgg gcaggagctg cccttcgcgc actacacgcc ggcgagtgat
     4621 cgcacaccgc tcccctccgc cgaagtcatc cggacctatc aggaccgcga cggctacctc
     4681 tggttcgtca tcttctcctc cggcatcgtt cgctacgacg gccactcgtt cgagacctac
     4741 accatctccg atggattgag ggatctcgcc gtttgggaga tcgtcgagga ccgctcgggg
     4801 cggctctggg tggggtcgaa cgcgggcctc gtggtctcgg agcgcccgct cggcgagtat
     4861 cctcccggcg agcgcattcg tttcgtggtg agcgtggggg gcgtcgacct catcgacacg
     4921 ctgatcaatc gaaaccggct ggtcgccgac gcgcgcgggc gcgtgtgggc ggggacgtcg
     4981 tcgacgggga tcgtccgtta ccacgtcgcg tccgacgggg actcgatacg aacgacgtgc
     5041 gacaccctca cgaccgaccc gcacggcgag ggcaagaacg ccaccgtgcg ctcgatcgcc
     5101 gtgcgtcgcg acgggtccgt gtgggcgagc atcggcggcg gcatgctgct ggaattccgc
     5161 gacgatacga gtaccgtctc cgcgatctcg caagagtcgg gactcccgac gcagagcatc
     5221 acgaccctct acgagtctcc gcggggcgta ctgtggggcg ggtgccgtaa cgggctgatc
     5281 tggcgcctcg acgagacgag cgacacgcgc cgcgtcgtga ccgtcaacga acggctcagc
     5341 gcgagtatcg ccgcgatgct ggaaacgtcg gatagcacgc tctgggtcgc gagcgacggc
     5401 gccggcgtct tgacgctcga cccggcacac cccgagcgcg cctcccccat cacgcgcgcc
     5461 gacggactct tgagcgacaa cgtccatcac gtgcagctcg atcgcgaagg ctccctgtgg
     5521 ttctcccagt cgggcggggc gtccaagctg cgcaccaact acgaagcgtt ccggaactac
     5581 accgccgcac cgcgctcgga tgtgccgtcg acgctcccgt caccggccgt gaacgtcgtc
     5641 attcctccag cggccacgaa ctattcggct ctctggctgg ggaccacgga aagcggcgtg
     5701 gtcgcgattc gcgccgacgg caccgtttcg attctcgatg tcgaacaggg acttcgcaac
     5761 aactgggtca acggactcgt gttcgacggg gcgggc
//
LOCUS       DASPGK010000135         6997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1322738, whole genome shotgun sequence.
ACCESSION   DASPGK010000135 DASPGK010000000
VERSION     DASPGK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322738"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="VEC56_03885"
     CDS             <1..1749
                     /locus_tag="VEC56_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV13326.1"
                     /translation="IAGQLFRDQAVTVSVGQFAALAVALTAIPMLAALGARVRRADVA
                     GVSSDKLDQEDAHAVLSLGRFSQAYDRFVRRAIARKKTTVAIALGIFAAALAALPLIG
                     RELIPPLTEGEFFFEVSLPQGSSLAATDRVMTELEAAAAADPRVEQSYARVGSRLVAG
                     GVSLNTVGEHFGQINVVLKNKSDDRVEAAAVETLRQHFAEIPDVTAKFGRPSYFSVKT
                     PVEVVLYGESLEELRDYSLDLSRELATVPGLADVRSSLEAGNPELHVVFDRDRLAALN
                     LDLGTMSSTLQNRVQGVVPTRFKESDRQIDVRVRNQESDRASVEDVRNLVLPGPSGTP
                     IRLVSIADVRMDEGPAEIHRLQQQRAAVVSANLKGRSLGAAVDDIRASLAHSPPPSGI
                     TVELGGQNAEMAVSFRSLRFAMILAIFLVYLVMAATFESFVHPFIVLFSVPLAVIGVV
                     IGLLVTGTDISVMVLIGVILLVGVVVNNAIVLIDAINRLRRAGLEKSEAVVRAGHIRL
                     RPILMTTLTSVLGLLPMAISWGEGAELRAPLAITVLFGLSVCTLLTLVVIPAIYNWIP
                     SRVTVEAPVAPAVAEQ"
     gene            1761..4844
                     /locus_tag="VEC56_03890"
     CDS             1761..4844
                     /locus_tag="VEC56_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV13327.1"
                     /translation="MTLPEIAIKRHVTMLMLLVSLVVLGLVAVFRLPLAFMPDFENPM
                     LFVRLPYPNASPAQIERLVVRPVEEAVGSVSGMKNMWSHIDDGGGTIGLEFDWAQNMK
                     MARTEVWEKIDRVRRDLPDDVGDITVSNNWDARESDMPIIEGRLSSKKDLSESYDLLE
                     RRIVKPLERIPGVASVRLDGVNPREVRINLDIAKVEAHGVDVRDVIGKLESSNFDQSL
                     GKISEGDARWSLRMVGTFDDLDQIQNFPINETGLRLRDIADVRYEEPPLIYGRHLDGD
                     FAIGVTIAQESKANTVEVCDAVQARIAEMNTDPELEGVNFLTWFSQGDEIKKTLRDLT
                     FTGIFGAILAAMVLFAFLRRVSTTLVSVACIPFSLIVACGFVWARGGSMNTLVLLGLI
                     VAIGMLVDNAVVVMENIFRHQEQGEDPKTATSKGAAEVSIAVITGTLTSVIVFLPLIF
                     NKPSEMNVYLKELGIAVCLVILASLFISQTLIPMATSWFIRSKPKPKSKTLIKAEGVY
                     ERMLVFNLKHRWLAPIVMVAVLASAYFPFSKVEKNFDTSESELFVQVNYNPSESISLE
                     RKEELVNQVEALLEPHREELMARSIYSFWSDNWSMTRIYLKEGEANEENIATVRKKLR
                     ELLPEIAGVKVEVQENGQFWRQDRGKRVALQLVGEDSEVLAGLADDARTRIEGIPGLV
                     DPFSGSEERQQEIHVNLDRELASRMGIASTQPAEVVGLTFRGQRLQRFRTPDGEREMR
                     VTLDEEETETVAQLRNLPIWTEEGEKIPLASLATFDEKPGEQWINRDNRLTSVWVGAR
                     FEEGTREDYIPLVTAAMNGIDFPYGYSWTFGRWEERRKENAQEFLTNLYLALLLIFAV
                     MASLFESARQAWTLLISLPFALAGAFWTLYLTGTDFDQPAAVGLLLLIGTVVNNGIVM
                     LAHVNHYRNEGMPRRDAMLRGCRERLRPVIMTALTTLVSLVPMVVQKPSLGGVYYYSM
                     ALVIMGGLSLSTFLTLILLPTATTLTEDSTSWVARMLGKLGVLLRLRKKHEVLAD"
     gene            4841..5782
                     /locus_tag="VEC56_03895"
     CDS             4841..5782
                     /locus_tag="VEC56_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HXV13328.1"
                     /translation="MTARSLRTAAICLVALAPVPRVDDARAQQVADSLFSPRIESPAY
                     PTEHGPVVLLDEAHHNFHTSGGRFFAFAKTLRRDGYIVRPLRASVTRAALDSARVFVI
                     SNALHAQNEEDWFLPTPSAFTDDEIAAVKAWVEDGGSLFLIADHMPFPGAVEKLARVF
                     GVLFANGFALNADEESGRMTYRRRDGSLADHPITRGRNRAERVDSLVTFTGQAFWLEG
                     RGDPLLRLARDAVLLMPEEAWMFSKRTPRLSASGTLQGAALRVGKGRVAVFGEAAMFS
                     AQVSGPQRAPMGMNDPTAGQNPQFLLNVVHWLSGVLK"
     gene            6026..>6997
                     /locus_tag="VEC56_03900"
     CDS             6026..>6997
                     /locus_tag="VEC56_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13329.1"
                     /translation="MPTHAAQPSRSIYAGIFAVSAATLLFQVAFVRIFSVSIWYHFAF
                     LVVSIALFGIGASGVALSFLKPGPRDTRYRALAPACFSASALLAYLLTNAIPFSPFRI
                     LQEPKQLFFFLVYDVLLMAPFFFSGAAVALILRAFPARAGRLYAFDLVGAAVGTLLVF
                     FVLPIGGARGAIALASALGLVSAAFLTPAWGGRRSLVMVGPACVLLVLGARALPDVRM
                     DSTKPVTTEVNQRGGSHVVSRWNALSRIDVVEKPGANPMIFIDAAAATPVMPPQDSTQ
                     AARDISTIGYRLRAGAAVAVIGSGGGVDVQNALALGARSVTAIEINPI"
BASE COUNT         1113 a         2236 c         2356 g         1292 t
ORIGIN      
        1 atagcgggcc agttgttccg cgaccaggcg gtgacggtga gcgtgggaca gttcgccgcc
       61 ctggcggtgg cgctgaccgc catccccatg ctcgccgcac tgggcgcgcg cgtgcgcagg
      121 gccgacgtgg ccggtgtgag ctcggacaag ctggatcaag aggacgcgca cgcggtgctc
      181 tcgctcggtc gtttctcgca ggcgtacgac cgcttcgtgc ggcgcgcgat cgcgcgcaag
      241 aagaccacgg tggcgatagc gctcgggata ttcgcggccg cgctagccgc gctcccgttg
      301 atcgggcgcg agctgattcc gcccctcacc gaaggtgagt ttttcttcga agtgagcttg
      361 ccgcagggct cgtcgctggc cgcgaccgac cgtgtgatga ccgagctcga agcggccgcg
      421 gccgccgacc cgcgcgtcga gcagagctac gcgcgcgtgg gtagccgcct ggtcgcggga
      481 ggcgtttcac tcaacacggt gggcgagcac ttcgggcaga tcaacgtggt cttgaagaac
      541 aagtccgacg accgcgtcga agcggccgcg gtcgagacgc tgcgccagca tttcgccgag
      601 attcccgacg tcaccgcgaa gttcggccgg ccctcgtact tcagcgtcaa gaccccggtc
      661 gaagtcgtgc tctacggcga gagcctcgag gagttgcgcg attactcgct cgacctgtcg
      721 cgcgagctgg ccaccgtgcc ggggctggcc gacgtgcgct cgtcgctcga agcgggcaac
      781 cccgagttgc acgtggtctt cgaccgcgac cgtctggccg cactcaatct ggatctcggc
      841 accatgtcgt cgacgctgca gaaccgcgtc cagggcgtgg taccgacgcg cttcaaggaa
      901 tcggaccgcc agatcgacgt gcgggttcgc aaccaggagt ccgaccgcgc ctcggtcgag
      961 gacgtgcgca acctggtgct gcccgggccg agcgggacgc cgatccgttt ggtttcgatc
     1021 gccgatgtgc gcatggacga gggcccggcc gagatccatc gcctgcagca gcagcgcgcg
     1081 gccgtggtca gcgccaacct caagggccgc agcctcggcg ccgcggtgga cgacattcgc
     1141 gcgtcgctcg cgcatagccc gccgccgtcc gggatcaccg tcgagttggg cggtcagaac
     1201 gccgaaatgg cggtgtcgtt ccggagccta cgcttcgcga tgatcctcgc gatattcttg
     1261 gtctacttgg tgatggcggc gaccttcgag tcgttcgtgc accccttcat cgtgctcttc
     1321 tcggtaccgc ttgcagtgat cggcgtggta atcggactcc tcgtcaccgg caccgacatt
     1381 tcggtgatgg tgttgatcgg cgtcattctg ctggtgggcg tggtggtcaa caacgcgatc
     1441 gtcttgatcg acgccatcaa ccggctgcgc cgcgccggcc tcgaaaagtc ggaagcggtg
     1501 gtgcgggccg gtcacatccg cctgcgcccc attctcatga ccacgctcac ctcggtgttg
     1561 ggtctcttgc cgatggcgat ctcgtggggt gaaggcgccg agctgcgcgc gccgctcgcc
     1621 attacggtac tgttcggact ttccgtgtgc acgttgctca cgttggtcgt gatccccgca
     1681 atctacaact ggattccttc gcgcgtgacg gtggaagcgc ccgtcgcgcc tgcggttgct
     1741 gaacaatagg caggtgtgac atgactctcc cggaaattgc catcaagcgt cacgtgacga
     1801 tgctcatgct gctggtgagc ttggtcgtat tgggactggt cgcggtgttt cgtctgccgc
     1861 tcgcgttcat gcccgacttc gagaatccca tgctgttcgt gcgccttccg tacccgaacg
     1921 cgtcgccggc ccagatcgag cgcctggtgg tgcggccggt cgaagaagcg gtgggctcgg
     1981 tgagcggcat gaagaacatg tggtcgcaca tcgacgacgg cggtggcacc atcggactcg
     2041 aatttgactg ggcgcagaac atgaagatgg cgcgcacgga agtgtgggag aagatcgacc
     2101 gcgtgcgccg cgatcttccc gacgacgtcg gcgacatcac ggtctcgaac aactgggacg
     2161 cgcgcgagtc cgacatgccc atcatcgagg gacgcctgtc gtccaagaag gacctctcgg
     2221 agagttacga cctgctcgag cgccgcatcg tcaagccgct cgagcgcatc cccggcgtgg
     2281 ccagcgtacg cctggacggc gtgaatccgc gcgaagtgcg catcaatctc gacatcgcca
     2341 aggtcgaggc gcacggcgtc gacgtccgcg acgtgatcgg caagctcgag agcagcaact
     2401 tcgaccagtc gctcgggaag atatccgagg gtgacgcacg ctggtcgcta cgcatggtag
     2461 ggacgttcga cgacctcgac cagatccaga actttcccat caacgagacc ggtttgcgcc
     2521 tgcgcgacat cgccgatgtc cgctacgaag agccgccttt gatctacgga cgccacctcg
     2581 acggtgactt cgccatcggt gtgaccatcg cgcaggagtc gaaggccaac acggtggagg
     2641 tgtgcgacgc agtgcaggcg cgcatcgcgg agatgaacac cgatccggag ctggaggggg
     2701 tcaacttcct gacctggttc agccagggcg acgagatcaa gaagacgctg cgcgatctca
     2761 cgttcacggg catcttcggg gccattttgg cggccatggt tctgttcgct ttcctgcgcc
     2821 gcgtgtcgac caccctggtc tcggtggcct gcattccgtt ctcgctcatc gtcgcgtgcg
     2881 gtttcgtgtg ggcccgcggt ggatcgatga acacgctggt gctgttgggc ctcatcgtgg
     2941 ccatcggcat gctggtcgac aacgcggtgg tggtgatgga gaacatcttc cgccaccaag
     3001 agcagggcga ggaccccaaa acggccactt cgaagggcgc ggccgaagtc tccatcgcgg
     3061 tgatcacggg gaccctgact tcggtgatcg tgtttctccc gctcatcttc aacaagccga
     3121 gcgagatgaa cgtgtatctc aaggagttgg gtatcgccgt gtgcctggtg attctggcct
     3181 cgctgttcat cagccagacg ctcatcccca tggcgacgtc gtggttcatt cgttccaagc
     3241 cgaagcccaa gtcgaagacg ctgatcaaag ccgagggagt ctacgagcgc atgctcgtgt
     3301 tcaatctaaa gcaccgctgg ttggcgccga tcgtgatggt ggcggtgctg gcctccgcgt
     3361 acttcccgtt ctccaaagtc gagaagaact tcgacaccag cgagagcgag ctgttcgtcc
     3421 aggtcaacta caaccccagc gaaagcatca gcctggagcg caaggaagag ctggtcaatc
     3481 aagtcgaagc cctgctcgag ccccatcgcg aggagctgat ggcgcgctcg atctacagct
     3541 tctggagcga caactggtcg atgacacgga tttatctcaa ggaaggcgag gccaacgagg
     3601 aaaacattgc cacggtgcgc aagaagttgc gtgagctcct gccggagatc gccggagtca
     3661 aggtcgaggt gcaggagaac ggccagttct ggcgtcagga ccggggcaag cgcgtcgccc
     3721 tccagttggt gggcgaagac tcggaagtgc tcgccggcct cgccgatgat gcgcgtactc
     3781 gcatcgaagg gataccgggg ctggtcgatc cgttctcggg cagcgaggag cgccagcagg
     3841 agatccacgt caacctggac cgcgagcttg cgtcgcgcat gggcatcgcg tccacccagc
     3901 cggccgaggt ggtcggactg acgttcagag gccagcgttt acagagattc cgcacacccg
     3961 acggcgaacg cgagatgcgc gtgacgctcg acgaggaaga gaccgaaacc gtcgcgcagc
     4021 tgcgcaacct gcctatctgg accgaggaag gcgagaagat cccgcttgcg tcgctcgcca
     4081 ccttcgacga gaagccgggc gagcaatgga tcaaccgaga caatcggttg accagcgtgt
     4141 gggtgggcgc gcgcttcgaa gagggaacgc gcgaagacta cattccgctc gtcaccgcgg
     4201 ccatgaacgg gatcgacttc ccgtacgggt actcgtggac gttcggccgc tgggaggaac
     4261 gccgcaagga gaacgcgcaa gagttcctca ccaacctgta cctcgcgctg ctcttgatct
     4321 tcgccgtgat ggcgtcgctg ttcgagtccg cgcgccaggc gtggacgctc ctgatttcgc
     4381 tgccattcgc gctggcgggc gcgttctgga ccttgtacct caccggcacc gacttcgatc
     4441 agccggccgc ggtgggactg ctgctgctga tcggcaccgt cgtcaacaac ggcatcgtga
     4501 tgctggccca cgtgaaccac taccgcaacg agggcatgcc gcgccgcgac gccatgctgc
     4561 gcgggtgccg cgagcgcctg cgccccgtca tcatgaccgc gctgacgacg ctggtgtcgc
     4621 tggttccgat ggtggtgcag aagccgtcgc tgggcggcgt gtactactac tcgatggcgc
     4681 tcgtgatcat gggcgggttg tctctctcga cgttcctcac cctgatcctc ctgccgacgg
     4741 cgacgacgct cacggaggac tcgaccagct gggtggcgcg catgctcggg aagctgggcg
     4801 tgctgctgcg gctccgcaag aagcacgagg tgctggctga ttgacggcac gatcgctccg
     4861 caccgcagcc atttgcctgg tggcgttggc gcccgtgccg cgggtcgacg acgcgcgcgc
     4921 ccagcaggtg gcggattcgc tcttctcgcc gcgcatcgag tcgcccgcct accccacgga
     4981 gcatggcccg gtagtcctgc tggacgaggc gcatcacaac ttccacacct ccggcgggag
     5041 attcttcgcg ttcgcgaaga cgctgcgccg ggacggctac atcgtccgtc cgctgcgcgc
     5101 gagcgtcacg cgcgcggctc tcgattccgc gcgcgtgttc gttatctcca acgcgctgca
     5161 cgcgcaaaat gaggaagact ggttcctgcc gactccatcc gcgttcacgg acgacgagat
     5221 cgcggcggtt aaggcgtggg tcgaggacgg cgggtcgctg ttcctgatcg cggaccatat
     5281 gccgtttcca ggcgcggtgg agaagctcgc gcgtgtgttc ggcgtcctgt ttgccaacgg
     5341 gttcgcgttg aacgcggacg aggagtcggg acgaatgacg tatcgccggc gcgacggctc
     5401 gctcgccgat cacccgatca cgcgcgggcg caatcgagcc gagcgcgtgg actcgttggt
     5461 caccttcacg gggcaggcat tttggttgga agggcgcggc gatccgctcc ttcgcttggc
     5521 gagggacgcg gtgctcctca tgcccgaaga agcgtggatg ttctcgaagc gcacgccccg
     5581 gctctccgcg tcgggcacgt tgcagggcgc agccttgcgg gtggggaagg gtcgggtggc
     5641 cgtgttcggc gaggccgcga tgttctcggc ccaggtgtcc gggccgcagc gtgcaccgat
     5701 gggcatgaac gatcccacag ccgggcagaa cccgcagttc ctgctcaacg tggtgcactg
     5761 gctgagcggc gttctgaagt gacccgcgcg gcgcgggctc gatgccatcg ccggtctcat
     5821 ggtccggata gaggtcgttg tcggatcagc tcgtgggcgc cgacgacgga gtggacgttc
     5881 acccagccta cctcaccacg tcgaaggcct cgatgtccgc gcggctctcg cggctcagtg
     5941 cgaagtcgac gcgcaggcgg gtcccgaggt cgcggattga tgctgtcaag gccacgcgac
     6001 gtgggttagg cttgcgctcg atcgcatgcc gacccacgcc gcccaacctt cccggtccat
     6061 ctacgcgggc atcttcgccg tgtcggcggc gacgctgctc ttccaggtcg cgttcgttcg
     6121 catcttctcg gtctccatct ggtatcactt cgcctttctc gtggtctcga tcgccctctt
     6181 cggcatcggg gccagcgggg tggcgctgtc gttcctgaag cccggtccgc gcgatacgcg
     6241 ctatcgcgcg ctcgcccccg cgtgtttctc ggcctcggcc ctgttggcgt atctcttgac
     6301 caacgcgatc ccgttctcgc cgtttcggat tctccaagag ccgaagcagc tcttcttctt
     6361 cctggtgtac gacgtgctgc tgatggcgcc cttcttcttc tcgggcgcgg cggtggcgtt
     6421 gatcctgcgc gcgttcccgg cccgggcggg gcggctgtac gcgttcgact tggtgggtgc
     6481 ggcggtgggg acgctgctcg tgttcttcgt acttccgatc ggcggcgcgc gcggggccat
     6541 cgcgctcgcg tccgcgctgg gcttggtctc ggccgcgttc ctcacgccgg cgtggggcgg
     6601 gcggcgatcg ctggtcatgg tggggccggc gtgcgtgctg ctcgtgctcg gcgcgcgcgc
     6661 ccttcccgac gtgcgcatgg actcgaccaa gccggtcacc accgaggtca atcagagggg
     6721 cggatcgcac gtggtgagcc gctggaacgc gctctcgcgc atcgacgtgg tcgagaagcc
     6781 cggcgccaac ccgatgatct tcatcgacgc ggcggccgcg actccggtga tgccgccgca
     6841 ggattccacc caggcggcgc gtgacatcag caccatcggg taccggctgc gcgcgggcgc
     6901 tgcggtggcg gtgatcggat ccggcggcgg cgtcgacgtg cagaacgcgc tggcgctggg
     6961 agcaagatcg gtcaccgcga tcgagatcaa cccgatc
//
LOCUS       DASPGK010000136        10123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_621124, whole genome shotgun sequence.
ACCESSION   DASPGK010000136 DASPGK010000000
VERSION     DASPGK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621124"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VEC56_03905"
     CDS             <1..519
                     /locus_tag="VEC56_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13330.1"
                     /translation="GMCSRAIGILERVGGNDEFALHALDCLGDCYLNRKDLANATRVY
                     ERLRSISPSDQRGVAGLARCAAMTGDYATAERMLHEIVDPRRAVYPPAYIALAEVQRA
                     QGKIDEAIASYSHIAQLLGYQRQGYLALVELYRQKGDVKSALAALDKAATYSPPNDPT
                     IQGLFAALRSGR"
     gene            516..1490
                     /locus_tag="VEC56_03910"
     CDS             516..1490
                     /locus_tag="VEC56_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13331.1"
                     /translation="MSPARRGRRIGVIDVGTNSTKLVVGVVRGDRVVVLHFARRPSRL
                     GERLTHTGRISAVAADRTARDVRALSLLAQSHGAEVVVAVGTYAFRKASNGEAVARRI
                     ARRADVPLRVLAGREEAMLAYLSVLTRLRRPKPYTFLIDVGGGSTEFVAARRGRLERA
                     RSLPLGALRLTERHLRSDPVRPAERRALERSVDTAVSRVVAPFRRVPASQIDLIASGG
                     SATTALAMLAPRARRPATARVSRRALETLADACFARSLAQRKKMRGLPADRADIIPAG
                     LVVVLSFLRATGKRTLSVSDGGVREGVLIAIDAELRALAHERHSHRPI"
     gene            complement(1483..2172)
                     /locus_tag="VEC56_03915"
     CDS             complement(1483..2172)
                     /locus_tag="VEC56_03915"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXV13332.1"
                     /translation="MAIDILYEDNHIIAVNKPAGVLTQGDRTGDETALDLGKAYIRST
                     SHRPGNVFLGLVHRIDRPVSGVLVFARTSKAASRLARSFHDRQVEKVYLAVVMGRFEK
                     DTGELEGFIERTHLRSRLASAPSSKAKAAVLSYRVLATRDALSLLEVTPRTGRHHQIR
                     LQLSAAGHPVVGDLKYRAPRALPDKSIALHSARLAFPHPVREETITISAPPPEHEPWS
                     LFRDAIAAYFI"
     gene            2241..3167
                     /locus_tag="VEC56_03920"
     CDS             2241..3167
                     /locus_tag="VEC56_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV13333.1"
                     /translation="MIEVTNLSKSYGKVEAVRDVTFAVRPGEIYGLLGPNGAGKSTTI
                     HILSGLIAASAGTAKVGGHDIAREPLAAKHILGVVPQQSVVIDELSALANCMFFGALY
                     GVDKGVLRDRARGLLDWIELSDHLNKPASALSGGMLRRLTLVLGIIHEPKALILDEPT
                     TGLDPQTRLLILDRVREVAAKGTAVLLTTHHLEEAERLCHRVGIIDGGRIIKEGTLAE
                     LRAEVEDVQLFSLRGGFARERLREAVARLAGAEIVLDDANEVVVAVPTGSGLTARLIE
                     TAASLDLVQEVAIKPPSLESLFIRLTGRELRE"
     gene            3172..4425
                     /gene="arcA"
                     /locus_tag="VEC56_03925"
     CDS             3172..4425
                     /gene="arcA"
                     /locus_tag="VEC56_03925"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HXV13334.1"
                     /translation="MTQQYRVDSEVGKLRKVLVHRPDLSLRRLTPSNREELLFDDVLW
                     VRKARGEHDKFVGHLERRGVEVFYLDDLLADVLKNPEARSFVIDKTVTRLTVGPSLVK
                     EFRAYLFEMDPRRLATILIGGLAREEIDESLFGSHSLTLTTMDQTDFLLPPLPNSIFT
                     RDSSAWIFNGVSLNPMYFEARQLEVVHVAAIYNWHPMFVDADFEFWYPDQDEQGRFVQ
                     EDWGRASLEGGDVMPIGNKTVLMGIGERTTGQMVERLAQSLFRHGAAERVIACVMSKD
                     RAHMHLDTVFTMLDRDAITVYPRVVDQTRAYSLRPSDKPGRMELTEEKSFLDAVADAL
                     NVGKLRVIGTGGDEYQAEREQWDDGNNLIAIEPGVVVAYSKNEYTNKRLRKAGIKVLT
                     IDGSELGRGRGGGHCMTCPLLRDPI"
     gene            4442..5437
                     /gene="argF"
                     /locus_tag="VEC56_03930"
     CDS             4442..5437
                     /gene="argF"
                     /locus_tag="VEC56_03930"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXV13335.1"
                     /translation="MNLRNRHFLKDLDFTKEEIVYLLDLSKQLKAEKKERRETRRLSR
                     RNIALIFEKTSTRTRCAFEVAAFDQGAHVTYLGPSGSQIGHKETMKDTARVLGRMYDA
                     IEYRGYGQDIVEALAAHAGVPVYNGLTDDYHPTQMLADVLTMMEHCDRPVEKISFCYL
                     GDTRNNVSNSLLAGAAILGMDVRLAGPKECWPLESLLETCRKLAHESGARITLTDDAR
                     AAVRGADYLYTDVWVSMGEPESKWSERIRLMQPYQVNRALMEATGNARAKFLHCLPAF
                     HNRDTKVGESIFQQYGLDGLEVTEEVFESPASIVFDQAENRLHTIKAILVATLGD"
     gene            5442..6353
                     /gene="arcC"
                     /locus_tag="VEC56_03935"
     CDS             5442..6353
                     /gene="arcC"
                     /locus_tag="VEC56_03935"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXV13336.1"
                     /translation="MARRRIVIALGGNALLKRGEPMTVANQRENVRIAARAIAPVTAD
                     NDLVISHGNGPQVGLLALQAAASGQHTPLDVLGAETEGMIGYMIEQELGNLVPFERPF
                     ATMLTMVEVDPADPAFQNPTKPIGPMYTKDQAAMLSTEKGWTVAEDGAGWRRVVPSPL
                     PKRIFEMRPIKWLLEHHTIVICAGGGGIPTLYRPDGTLTGAEVVIDKDRASAVLAREV
                     GADLFVMATDVTGVYADWGTSKHRLLRNTTPAELRALRFAAGSMGPKVEAAIAFVEET
                     GRRAAIGSLEEIERIVAGQAGTIVERG"
     gene            6360..7571
                     /locus_tag="VEC56_03940"
     CDS             6360..7571
                     /locus_tag="VEC56_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV13337.1"
                     /translation="MHLFRMYWQRVLKRPGSALLWMALPFAFMTIYTLAFGGGDGGMP
                     KVGIAIVDQDSSLVSGLVKTSLAQGPVAGMVTLVEARDLTEVEALFEDETASAAIVVP
                     DGFADKMLRSEPDTLLLYRNPRHTIGPQIAEGILGGMVTIGNELLGQFSAPLRSARNM
                     LDQPTAPSSEEVASLSNAFFAAGRRTQGLSSLRQIDVIVVDEEAKEASGFSTAALFFP
                     GLIMFGLLSLSLHLEHRFILDRTRHVTDRLVTAPVAPWRIAIEQRVFALSFLYLIGIV
                     SLTLGAVIWRIPLRGVASANLILVAMVIFIAGWNGLLYALSRSPRAASAIASTAMVFL
                     SILGGGFFPAEFMPASFLSAVKWVPTGMANLGLTRALTGRDVTISLPLLFAMSLTVLT
                     MAVLLSRKRIR"
     gene            7571..8674
                     /locus_tag="VEC56_03945"
     CDS             7571..8674
                     /locus_tag="VEC56_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV13338.1"
                     /translation="MRSSNAWTIAKLELTLALRDKEAVVWSLIAPIVMAAIFGAMFGG
                     NEPPAPTRVTLEAGTNPPVVRDAFTTLLERRGLEVGEGGIRVQLPDSLIDRMLDGRGA
                     TARVVKKDETDIRAMGVSASVREAMVVFAFRAGDLRGRADAAVALESTGPLAVHASTL
                     GAAPRIVSGKEHTLPAMLVMFIMFQLTTFFMVLWVDDLNTGKTKRITMSPVRTREILA
                     GNVAARLVWATMQVAVILGMGALVLGVRLAINPAHFAVVIAAYMLAATALGLLLGTCF
                     KSSEKAGAAGVISGLMMAALGGCWWPLEIVPGAMRAVALFLPTGQAMDAIGEMTALGA
                     AAPFPMRNVIVLLAMTAIVLPIATRRMRVQLTS"
     gene            8698..>10123
                     /locus_tag="VEC56_03950"
     CDS             8698..>10123
                     /locus_tag="VEC56_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXV13339.1"
                     /translation="MSSRVHSILTSSRVAIACAVVAAVALAASARDGSVSADRMLAHV
                     KYLASDELEGRAPGSRGEQLTVAYLTDQLEKAGCAPGNPDGSYTQKVPMYGFEVTNSP
                     ALVVRNKDGQSEKRYAAGKEFVGWTVQQKPATSVRDAELVFVGYGVDAPEYQWNDFKT
                     DVRGKVIVILVNDPPMADASLFGGKAMTYYGRWTYKFEQAAAVGATGAILVHNTVDAG
                     YPWAVVENSWSGEQFDVVRTDQGASRCAFESWISEDAARDLFMAAGRSFDDDKKSAAA
                     REFQPVSLGRLASVDIKTRSRTIESSNVVASVKGSGQTADEWIVYTAHWDHLGVGKAA
                     DNDSIYNGALDNASGVAGVMEIARAFASHGRPLGRSVLFIFTTGEESGLLGATHYTNH
                     PLYPLEKTIAEINIDGLNIWGRTEDMIVVGWGQSDLEQTLADAILTMNRRIAPDSEPE
                     KGYYYRSDHFPFAKKGVPALYADSG"
BASE COUNT         1660 a         3426 c         3377 g         1660 t
ORIGIN      
        1 ggcatgtgct cgcgcgcgat cgggatcctc gagcgcgtgg gcggcaacga cgaattcgcg
       61 ctccacgcgc tcgactgcct cggcgactgc tacttgaatc gcaaggacct cgccaacgcg
      121 acccgcgtgt acgagcgact gcggtcgatc tcgccctcgg accagcgcgg agtcgcgggg
      181 ctggcgcgct gcgccgccat gaccggcgac tacgcgacgg ccgaacgcat gctgcacgag
      241 atcgtcgacc ccaggcgcgc ggtgtatccg cccgcgtaca tcgcgctggc cgaggtgcag
      301 cgggcgcagg gcaagatcga cgaagccatc gcgagctact cgcacatcgc gcagctgctc
      361 ggctaccagc gccagggcta cctggcgttg gtcgagttgt atcggcagaa gggcgacgtg
      421 aagtccgcgt tggcggcgct ggacaaggct gcgacctatt ccccgcccaa cgaccccacc
      481 atccagggtc tgttcgcggc gctgcgctcc gggcgatgag ccccgcgagg cgcggccgac
      541 gcatcggcgt catcgacgtc ggcaccaatt ctaccaagct cgtggtggga gtggtccgcg
      601 gcgaccgcgt ggtcgtgctc catttcgcgc ggcggccgtc gcggctgggc gagcgcctca
      661 cgcatacggg aagaatctcc gccgtcgcgg ccgatcgaac cgcgcgcgac gtgcgtgcgc
      721 tctcgctgct ggcgcaatca cacggcgccg aggtcgtggt ggcggtgggc acctacgcct
      781 tccgcaaggc gtccaacggc gaggcggtgg cgcggcgcat cgcgcggcgc gccgacgtcc
      841 ccctgcgcgt cctcgcggga cgcgaggaag ccatgctcgc ctacctctcc gtgctgacgc
      901 ggctgcggcg gccgaagccg tacacgttcc tgatcgacgt cgggggcgga tcgaccgaat
      961 tcgtggccgc gcgccggggc cggctcgagc gcgctcgctc gctgccgctg ggtgcgctgc
     1021 gcttgaccga gcgccacctc cgctcggatc ccgtccgtcc cgccgagcgg cgcgcgctgg
     1081 agcgatcggt cgacaccgcc gtttcccgcg tcgtggcgcc gtttcggcgc gtgcccgcgt
     1141 cccaaatcga cctcatcgcg tccggtggct cggccaccac cgcgctcgcc atgctggctc
     1201 cgcgcgcgcg tcgccccgcg accgcacgcg tttcgcgccg cgcgctcgaa accctcgccg
     1261 acgcgtgttt cgcgcgctcg ctcgcccagc gcaagaagat gcgcgggctc cccgccgacc
     1321 gcgccgacat catcccggcc ggactcgtcg tcgtcctgtc cttcttgcgc gcaaccggca
     1381 aacgaacgtt atcggtgagc gacggcggcg tgcgcgaagg cgtgctgatc gcaatcgacg
     1441 ccgagctgcg cgcgctggcc cacgagcgcc actcgcatcg gcctatatga agtaggcggc
     1501 gatcgcgtcg cggaagaggc tccacggctc gtgctcgggc ggtggggcgg aaatcgtgat
     1561 cgtttcctca cgcaccgggt gcgggaacgc gagccgcgcc gagtgcagcg cgatcgactt
     1621 gtccgggagc gcgcgcggcg cgcgatactt caagtcgccg accaccgggt gtcccgccgc
     1681 cgacaattga agccggatct ggtggtggcg ccccgtgcgc ggcgttactt cgagcagaga
     1741 cagcgcgtcg cgcgtcgcga gcacccggta cgaaagcacc gccgccttcg ccttcgacga
     1801 cggcgcggat gccaggcgcg agcgcaggtg cgttcgctcg atgaagccct ccagctcccc
     1861 cgtgtccttt tcgaagcgcc ccatcaccac cgcgaggtag accttttcga cctgacggtc
     1921 gtggaacgac cgcgcgagcc gcgacgccgc cttcgaggtg cgtgcgaaca ccaagacgcc
     1981 cgagacggga cggtcgatcc gatggacgag ccccaggaac acgttgccgg ggcggtgcga
     2041 ggtggaccgg atatacgcct taccgagatc cagcgcggtc tcgtcgcccg tccggtcacc
     2101 ttgcgtcaat acgcccgcgg gtttgttgac cgcgatgatg tggttgtcct cgtagaggat
     2161 gtcgatggcc agcgtcgctc cttcgcttga ccgcgctcgc cgcctcccct atactccccg
     2221 gattcggcca agtgcaaatc atgatagaag ttaccaacct atcaaaatcc tacgggaaag
     2281 tcgaggcggt gcgcgatgtc accttcgcgg tgcgccccgg cgagatttac ggcctcctcg
     2341 gacccaacgg tgcgggcaag tcgaccacga tccacatcct ctcgggtctg atcgcggcct
     2401 cggccgggac cgccaaggtc ggcgggcatg atattgcgcg cgaaccgctg gccgccaagc
     2461 acatcctcgg tgtggtgccg cagcagtcgg tcgtgatcga cgagttgagc gcgctcgcca
     2521 attgcatgtt cttcggcgcc ctctacggcg tcgacaaagg cgtgctgcga gaccgcgcgc
     2581 gcggcctcct ggactggatc gagctgtcgg atcacttgaa caagccggcg agtgcgttgt
     2641 cgggcgggat gctgcgacgg ctgaccctcg tgctcggcat catccacgag cccaaggcac
     2701 tgatcctgga cgagccgacc accggcctcg acccgcagac gcgcctcttg atcctcgatc
     2761 gcgtgcgcga ggttgcggcc aagggtaccg cggtgctgct gaccacgcac cacttggaag
     2821 aagcggagcg cctgtgccac cgcgtcggca tcatcgacgg cggccgcatc atcaaagagg
     2881 gcacgctggc cgagctgcgc gcggaagtcg aagacgtcca gctcttcagc ctgcgcggcg
     2941 gcttcgcgcg cgagcgcttg cgcgaggcgg tggcacggtt ggccggagcc gagatcgtat
     3001 tggacgacgc caacgaagtc gtggtcgcgg tgccgaccgg ctcggggctc accgcgcgct
     3061 tgatcgagac cgcggcctcg ctcgacctcg tgcaggaagt cgccatcaag ccgccttcgc
     3121 tcgaaagcct cttcatccgg ctcacgggtc gtgagctccg ggagtgattc gatgacgcag
     3181 cagtaccgcg tggactccga agtgggcaag cttcgcaagg tcctcgtgca tcgccccgac
     3241 ttgagcctgc gtcgcctcac gccgtcgaac cgcgaggagc tcctcttcga cgacgtgctg
     3301 tgggtacgca aggcgcgcgg cgagcacgac aagttcgtcg gccacttgga gcggcgcggc
     3361 gtcgaggtgt tctatctcga cgacctcctg gcggacgtct tgaagaaccc ggaagcgcgt
     3421 tcgttcgtga tcgacaagac ggtgacgcgg ctcacggtgg gcccgtcgtt ggtgaaggag
     3481 ttccgcgcct acctgttcga gatggacccg cgccggctcg cgaccattct gatcggcggg
     3541 ctcgcgcgcg aggagatcga cgagagcctg ttcggcagtc actcgctgac gctgaccacg
     3601 atggaccaaa ccgacttcct tctgccgccg ctgcccaact cgatcttcac gcgcgactcg
     3661 tcggcgtgga tcttcaacgg cgtgtccctg aacccgatgt acttcgaggc gcgccagctc
     3721 gaggtcgtgc acgtggccgc gatctacaac tggcacccga tgttcgtcga cgccgacttc
     3781 gagttctggt accccgacca ggacgagcag gggcgcttcg tgcaggagga ctgggggcgc
     3841 gcgtcgctgg aaggcggcga cgtgatgccg atcggcaaca agaccgtgct gatgggcatc
     3901 ggcgagcgca ccaccgggca aatggtggag cgcctcgcgc agtccttgtt tcgccacggc
     3961 gccgcggaac gcgtgatcgc gtgcgtgatg tcgaaagacc gtgcgcacat gcacctcgac
     4021 accgtgttta ccatgctcga ccgcgacgcc atcaccgtgt acccgagagt cgtcgatcag
     4081 acccgcgcct acagcctgcg tccgtccgac aaacccgggc gcatggaatt gaccgaggag
     4141 aagagcttct tggacgcggt cgccgatgcg ctgaacgtcg gcaaactgcg tgtcatcgga
     4201 accggcggcg acgaatacca ggccgagcgc gagcagtggg acgacggcaa caacctgatc
     4261 gcgatcgagc ccggcgtggt ggtcgcatat tccaagaacg agtacaccaa caagcgcttg
     4321 cgcaaggcgg gcatcaaagt attgaccatc gacggctccg agctgggccg cggccgcgga
     4381 ggcggacact gcatgacgtg cccgttgctg cgcgatccca tttgaggtgc aacgtaacgt
     4441 ggtgaatttg cgcaaccggc actttctcaa ggatctcgat ttcacgaagg aggagatcgt
     4501 ctacctcctc gacctttcga agcaactcaa agccgagaaa aaagagcgcc gcgagacgcg
     4561 gcgcttatct cgccgcaaca tcgcgctcat cttcgagaag acctcgacgc gcacgcgctg
     4621 cgccttcgag gtggccgcgt tcgatcaggg cgcgcacgtg acctacctgg gccccagcgg
     4681 ctcgcagatc gggcacaagg agacgatgaa agacaccgcg cgcgtgctcg gccgcatgta
     4741 cgacgccatc gagtaccgcg gctacgggca ggacatcgtc gaagcgctgg cggcgcatgc
     4801 gggtgtgccc gtctacaacg ggctcaccga cgactaccat cccacccaga tgctggccga
     4861 cgtgctgacc atgatggagc actgcgaccg gccggtcgaa aagatctcgt tctgctatct
     4921 gggcgacacg cgcaacaatg tgtccaactc gctcttggcg ggcgctgcga tcctgggtat
     4981 ggacgtgcgt cttgcgggcc cgaaggaatg ctggccgctc gagtctctgc tcgaaacgtg
     5041 ccggaaactc gcccacgaga gcggcgcacg tatcacactt accgacgacg cgcgcgccgc
     5101 ggttcgcggc gcggattatc tctacaccga cgtgtgggtc tcgatgggcg agcccgagtc
     5161 gaagtggagc gagcgcatcc ggctcatgca accgtaccaa gtcaatcgcg cgctcatgga
     5221 ggcgaccgga aacgcccgcg cgaagttcct ccactgcctg cccgcgttcc acaatcgcga
     5281 taccaaggtg ggcgagtcga tcttccagca atacggtctg gacggtctcg aagtgaccga
     5341 agaggtgttc gagtcgccgg cgtcgatcgt gttcgaccag gccgagaacc ggttgcacac
     5401 catcaaggcg atcctggtcg ccaccctcgg cgactagatc catggcccgt cgccgcatcg
     5461 taatcgcgct gggagggaac gccctgttga agcgcggcga gccgatgacg gtcgccaacc
     5521 agcgcgagaa cgtgcgcatc gccgcgcgcg cgatcgctcc cgtcaccgcc gacaacgacc
     5581 tcgtcatctc gcacggcaac gggccgcaag tgggattgct cgcgctgcag gcggcggcca
     5641 gcggacagca caccccgctc gacgtgctgg gtgcggagac cgagggcatg atcggctata
     5701 tgatcgagca ggagctgggc aacctggttc ccttcgagcg gccgttcgcg accatgctga
     5761 ccatggtcga ggtcgacccc gccgaccccg cgttccagaa tccgaccaaa ccgatcgggc
     5821 cgatgtacac caaggaccaa gcggcaatgc tctcgaccga gaaaggctgg acggtcgccg
     5881 aagacggcgc cggctggcgc cgtgtggtgc cttcgccgct gcccaagcgc atcttcgaga
     5941 tgcgccccat caagtggctg ctcgagcacc acaccatcgt gatttgcgcc ggcggcggcg
     6001 gcatcccgac cctgtaccgt cccgacggga cgctcaccgg cgccgaagtc gtgatcgaca
     6061 aggaccgcgc gagtgcggtg ctcgcgcgcg aagtgggcgc cgacttgttc gtgatggcga
     6121 ccgacgtcac cggcgtgtac gccgattggg ggacgtcgaa gcaccgtctc ctgcggaaca
     6181 ccacacccgc ggaactgcgc gcgcttcgct ttgcggcggg ctcgatgggc cccaaggtcg
     6241 aagccgccat cgccttcgtc gaagagacgg gccggcgcgc cgccatcggg tcgctcgaag
     6301 agatcgagcg catcgtcgcg ggccaggccg gcaccatcgt cgagcgcggg tagcacgcca
     6361 tgcacctgtt ccgcatgtac tggcagcggg ttctcaagcg accggggtcg gcgctgttgt
     6421 ggatggcgct ccccttcgcg ttcatgacca tctatacgct cgccttcggc ggcggtgacg
     6481 gcggcatgcc caaggtcggc atcgcgatcg tcgaccagga cagctcgctg gtgtcgggac
     6541 tggtgaagac gtcgctcgcg cagggaccgg tggccgggat ggtgacgctc gtcgaggcgc
     6601 gcgacttgac cgaagtcgaa gcgctcttcg aggacgagac cgcgtcggcg gccatcgtcg
     6661 tgccggacgg gttcgccgac aagatgctgc gatcggagcc cgacaccttg ctcctgtacc
     6721 gcaacccgcg ccacaccatc ggaccgcaga tcgcggaggg gatcctgggg ggcatggtga
     6781 cgatcggcaa cgagttgctg ggccagttca gcgcgccgct gcgttcggcg cgcaatatgc
     6841 tcgaccagcc gaccgcgccg tcatcggaag aggtcgcgag cttgtcgaac gccttcttcg
     6901 cggccggccg gcgcacgcaa ggcctgtcgt ccctgcgcca gatcgacgtc atcgtggtcg
     6961 acgaggaagc caaggaagca tcgggcttca gcacggcggc gctgttcttt cccgggctca
     7021 tcatgttcgg gctcttgtcg ctctcgctcc acctcgagca ccgcttcatc ctggatcgca
     7081 cgcgccacgt gaccgatcgg ctggtgaccg cacccgtcgc gccctggcgc atcgcgatcg
     7141 agcagcgggt gttcgcgctg tcgtttctct acctgatcgg catcgtgtcg ctcaccttgg
     7201 gcgcggtgat ctggaggatc ccgctgcgcg gtgtcgcgtc cgccaacctg atcctggtcg
     7261 cgatggtgat cttcatcgcg ggttggaacg ggctgctcta cgcgctgtcg cgctcgccgc
     7321 gcgccgcctc cgcgatcgca tccaccgcga tggtcttcct ctcgattctg ggcggcggct
     7381 tcttccccgc cgagttcatg cctgcgagct tcctttcggc cgtgaaatgg gtcccgaccg
     7441 gcatggccaa cctgggactg acgcgcgccc tcacgggccg cgatgtcacc atttcgctcc
     7501 cgctgctgtt tgcgatgagc ctgacggtac tgacgatggc ggtcctgctc tcgcgcaaga
     7561 ggatccgctg atgcgttctt ccaacgcgtg gaccatcgcc aagctcgagt tgaccctcgc
     7621 cctgcgcgac aaggaggcgg tggtgtggtc gctgatcgca cccatcgtga tggcggccat
     7681 cttcggggcg atgttcggcg gcaacgagcc gcccgcgccc acccgcgtca ccctcgaggc
     7741 gggtacgaat ccccccgtcg tgcgcgacgc gttcacaacc ctcctcgaac gacgcggcct
     7801 cgaggtaggc gagggcggga tccgcgtgca gctgcccgac agcctcatcg accgcatgct
     7861 cgacgggcgc ggggctaccg cgcgcgtggt caagaaggac gagaccgaca ttcgcgcgat
     7921 gggtgtgagc gcgtccgtgc gcgaggcgat ggtcgtgttc gcgtttcgcg ccggcgatct
     7981 tcgcgggcgc gcggacgcgg ccgtcgcgct ggaaagcacc ggtcctttgg ccgtgcacgc
     8041 gtccacgctg ggcgcggccc cgcgcatcgt ctccggcaag gagcacacgc tccccgcgat
     8101 gctggtgatg ttcatcatgt tccagctcac caccttcttc atggtcctgt gggtcgacga
     8161 cttgaacacc ggaaagacga agcgcatcac gatgagcccg gtacgcacgc gtgagatcct
     8221 cgccgggaac gtcgccgcac gactggtgtg ggcgaccatg caggtggccg tcatcctcgg
     8281 catgggcgcg ttggtgctgg gcgtgcgcct ggcgatcaat cccgcgcact tcgcagtcgt
     8341 gatcgcggcc tatatgctgg ccgccaccgc gctcgggctc ctgctcggga cctgtttcaa
     8401 gtcgagcgag aaggcgggag cggccggggt gatctccggc ctgatgatgg cggccctggg
     8461 cgggtgctgg tggccgctcg agatcgtgcc gggggcaatg cgcgccgtgg cgctctttct
     8521 ccccaccgga caggccatgg acgccatcgg cgagatgacc gcgttgggcg ccgccgcgcc
     8581 cttccccatg cgcaacgtga tcgtactgct cgcgatgacc gccatcgtgt tacccatcgc
     8641 gacccggcga atgagagtac aattgacgag ctagacagga atcgaaagga gatcctcatg
     8701 tcctcccgcg tgcactcgat actcacctct tcgcgcgtcg cgatcgcgtg cgcggtcgtc
     8761 gcggccgtgg cactggcggc gtccgcgcgc gacggatcgg tctcggccga ccgcatgctc
     8821 gcgcacgtga agtacctcgc ttccgacgag ctcgaggggc gcgcgccggg gtcgcgcggc
     8881 gagcaattga cggtcgccta tttgaccgac cagctcgaga aggcaggatg tgcgcccggc
     8941 aaccccgacg gcagctacac ccagaaggtc cccatgtacg gcttcgaggt gacgaactca
     9001 ccggcgctgg tcgtgcgcaa caaggacggc cagagcgaga agcgctacgc ggccggcaag
     9061 gagttcgtcg gctggaccgt gcagcagaaa ccggcgacgt cggtgcgcga cgccgagttg
     9121 gtgttcgtgg gctacggtgt ggatgcgccc gagtaccagt ggaacgactt caagaccgac
     9181 gtgcgcggca aggtgatcgt gatcctggtc aacgatcccc cgatggcgga cgcatccctc
     9241 ttcggcggca aggccatgac ctactacggc cgctggacct acaagttcga gcaggccgcg
     9301 gccgtgggcg cgacgggcgc gatcctcgtg cacaacaccg tggacgccgg ttacccgtgg
     9361 gcggtggtcg agaacagttg gtcgggcgaa cagttcgacg tcgtgcggac cgaccagggt
     9421 gcgtcgcgct gcgccttcga gtcgtggatc tccgaagacg ccgcccgcga tttattcatg
     9481 gccgcggggc ggtccttcga cgacgacaag aaatcggcgg ccgcgcgcga gttccagccc
     9541 gtttccctcg ggcgtctcgc ctccgtcgac atcaagacgc gctcgcgcac catcgaatcg
     9601 agcaacgtgg ttgcgagcgt caagggatcg ggccagaccg ccgacgagtg gatcgtctac
     9661 accgcgcact gggatcacct gggggtcggc aaggccgccg ataacgattc catctacaac
     9721 ggcgccctcg acaacgcgtc gggtgtggcg ggcgtcatgg agatcgcgcg cgccttcgcg
     9781 tcgcacggcc gcccgctcgg gcgctcggtg ctgttcatct tcaccaccgg cgaagaaagc
     9841 gggctgctcg gtgcgaccca ctacaccaac catccgttgt acccgctgga gaagacgatc
     9901 gcggagatca acatcgacgg gttgaacatc tggggccgca ccgaagacat gatcgtcgtg
     9961 ggctggggcc agtcggacct cgagcagacc ctggccgacg cgattctgac catgaaccgc
    10021 cgcatcgcgc ccgacagcga gcccgagaag ggttactact accgctcgga ccacttcccc
    10081 ttcgccaaga agggcgtccc cgcgctctac gccgacagtg gtg
//
LOCUS       DASPGK010000137         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_504223, whole genome shotgun sequence.
ACCESSION   DASPGK010000137 DASPGK010000000
VERSION     DASPGK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504223"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VEC56_03955"
     CDS             complement(<1..994)
                     /locus_tag="VEC56_03955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXV13340.1"
                     /translation="MSLPWRDVPEWSADDFVSALGAQLNAGARLCALFGVPEGEDTHM
                     VAVVAFDDGGTLAVGRSAPIRGRYHALTPRYPQAHLFERELWETHGLVPDGHPWLKPV
                     RVETGAGPAAIDFFKVDGGEVHEVGVGPVHAGIIEPGHFRFQCHGEEVLYLEIALGFQ
                     HRGIEAALAGGPHRATMSQMETIAGDTTIAHATAHAMVLEALAKVEAPLRAQWIRAIA
                     LELERLANHTGDLGALANDVAFLPTSAACGRIRGDFLNLTATLCGNRFGRGLVHPGGC
                     RLDLESDRAARVWDQLRIALAEADEAVSWLWESNSTRTRFEGVGVVTSEQAESLG"
     gene            complement(1030..2475)
                     /locus_tag="VEC56_03960"
     CDS             complement(1030..2475)
                     /locus_tag="VEC56_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXV13341.1"
                     /translation="MIEIALIVTPLLLAAIAVAWPSQRTRPWLLPAAGAVHTSLTLTL
                     LLRPPSEPVGAWLGFDELARAVLPMVSILFLVCSIYAVAYLRIRADQSNRLFVTMLLS
                     LHALFSFALQARHLGLLWIGVEAATLLTVPLLHFNNTARAIEATWKYLIVGGTGIALS
                     LLGSFCLGYASLHGGGSGDLTFAGLTSQGPALSRPWLVAAWVLLLAGYGTKMGLAPMH
                     TWKPDAYGEAPGIVGAILAGGATTVAFVAVLRVKQVVDAAGAGPMAERTLLAFGLFSM
                     VVAALFLLGTRDFKRMLAYSSVEHMGILAVGAAFGAAGLSAALFHVWNNGLTKGALFL
                     SAGNIRRAARARTVDTVSGMAWCSPISARLLIVGLLAVTAIPPFGPFFSELRVVGAGL
                     ATGHGGAVAVFVICVLLAFLGLTRLMFAIVDGRPRGPAREERRASGRETAGVVVPPLL
                     FLCLSLWLGLAAPSLLQQSWHAAATFLHPLR"
     gene            complement(2472..3113)
                     /locus_tag="VEC56_03965"
     CDS             complement(2472..3113)
                     /locus_tag="VEC56_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HXV13342.1"
                     /translation="MNDVHNLLVGVAMGLNLIGLASSRLTSLIRAAAVQGMVLGVLPL
                     LLGHGANWWVILIAAATVAVKGFLIPGLLRKALRSAHIDREVQPLIPFVPSLLLGAAA
                     TVGAVALGGVLPLRAEHAHSLIVPGAFATVVSGFLLLMGRAKAIAQVCGYLMLENGIY
                     MFGLLLIGSTPIMVEAGILLDLTVAVFVVGIIVDRIQREFDTLDTRRLTALRE"
     gene            complement(3110..>3341)
                     /locus_tag="VEC56_03970"
     CDS             complement(3110..>3341)
                     /locus_tag="VEC56_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 4"
                     /protein_id="HXV13343.1"
                     /translation="LIFSVLLTEAVVPLGGLSPAVVAVTLGGGVLLTAVSVGVVESMQ
                     ARLPFRRVPMLLTTAFLLCLFALMIAWERSPQ"
BASE COUNT          655 a         1165 c         1066 g          455 t
ORIGIN      
        1 atcccaacga ttccgcctgt tcgctcgtca cgacaccgac gccttcgaag cgcgtgcgcg
       61 tcgagttcga ctcccaaagc caggatacgg cttcatccgc ctcggccagg gcgatgcgca
      121 attgatccca cacgcgcgcc gcccgatccg actcgaggtc taatcgacac ccgcccggat
      181 gcaccagccc gcgtccgaac cgattaccgc acagcgtcgc ggtgagattg agaaagtcgc
      241 cgcggatgcg accacacgcg gcggacgtcg gcaagaatgc aacgtcgttg gccaacgcac
      301 cgaggtctcc ggtgtggttg gccaagcgct ccaactcgag cgcgatcgcg cgaatccact
      361 gcgcgcgtaa cggcgcttcc acctttgcga gcgcctcgag caccatcgca tgggcggtcg
      421 cgtgggcgat cgtcgtgtcc cccgcgatgg tctccatttg gctcatcgtc gcccggtgcg
      481 gtccgcccgc gagcgccgct tcgatcccgc ggtgctggaa tcccagcgcg atttcgaggt
      541 agagcacctc ttcgccgtgg cattgaaatc ggaaatgtcc cggttcgatg atgccggcgt
      601 gcacggggcc gaccccgact tcgtggacct cgcccccatc gactttgaag aaatcaatgg
      661 ccgcgggacc cgcgcccgtt tcgacgcgca ccggtttgag ccatgggtga ccgtcgggga
      721 cgaggccgtg cgtctcccac aattcgcgct cgaacaggtg cgcctgcggg tatcgcggtg
      781 tcagcgcgtg gtaccgaccc cggatcggcg cgctccgtcc gaccgccagc gtgccgccat
      841 cgtcgaaagc gaccacggcc accatgtgcg tgtcctcgcc ctcgggcacg ccgaacagcg
      901 cgcacaggcg cgcgccggcg ttcaactggg cgccgagcgc ggacacgaaa tcgtccgcgc
      961 tccactccgg cacgtccctc cacgggaggc tcatgccgtt tctcaacacg gcgaattcgc
     1021 gcgacgtatt catcggagcg gatggaggaa cgttgccgcc gcgtgccacg actgttgcag
     1081 caacgagggc gcggcgagac ccagccacag cgaaaggcaa aggaacaaga gcggagggac
     1141 gacgacaccc gctgtctccc tcccgctggc gcggcgttct tcgcgcgcgg gcccgcgcgg
     1201 gcgcccgtcc acgatggcga acatcaatcg cgtaagaccc aggaacgcca gcaagacaca
     1261 gatcacgaac accgcgaccg cacccccgtg acccgttgcc agtccggccc ccacgacgcg
     1321 cagttcgctg aagaagggcc cgaacggcgg gatggcggtg acggctagca atcccacgat
     1381 cagcagtcgc gcggaaatgg gcgagcacca cgccatcccc gacaccgtgt cgacggtccg
     1441 cgcgcgcgcg gcgcgacgaa tgttgccggc gctcaggaac agcgctccct tggtcagccc
     1501 gttgttccat acgtggaaca aggcggccga caggcccgca gccccgaatg ccgctcccac
     1561 cgcgagaatc cccatgtgct cgacgctgga gtaggcaagc atgcgcttga agtcccgcgt
     1621 gccaaggagg aacaacgccg ccaccaccat cgaaaacaac ccgaaggcga gcagcgttcg
     1681 ctccgccatc ggtcccgcgc ccgcggcgtc caccacctgc ttgacgcgca gcacggcgac
     1741 gaacgctacc gtcgtcgcac cgccggcgag aatggccccg acgatgcccg gcgcctctcc
     1801 gtacgcatcg ggcttccagg tgtgcatcgg cgcgagtccc atcttggtgc cgtaacccgc
     1861 aagcagcagc acccacgcgg ccacgagcca cgggcgcgac agcgccggtc cctggctcgt
     1921 cagacccgcg aacgtgaggt cgccgcttcc tccgccgtgc agcgacgcgt agccgaggca
     1981 aaacgagccg agcaatgaaa gcgcgatccc cgtcccgccg acgatcaggt atttccaagt
     2041 cgcttcgatg gcgcgcgcgg tgttgttgaa gtgcagcagg ggaaccgtca acagcgtggc
     2101 ggcctcgacc ccgatccaga gcaaaccgag gtgtcgcgct tgcagtgcga aactgaacag
     2161 agcgtgcagc gagagcagca tcgtgacgaa gagacggttg gattggtccg ctcgaatccg
     2221 cagatacgcg acggcgtaga tcgagcacac caggaacaag atcgaaacca tcggcaacac
     2281 ggcccgcgcc agctcgtcga atccgagcca cgcgccgacg ggttccgagg gcggccgcag
     2341 cagcagggtc aacgtgagcg acgtgtgaac cgctccggcg gccgggagga gccacggtcg
     2401 cgttcgctgc gatggccaag ccaccgcgat cgccgccaac agcagcggtg tgacgatgag
     2461 ggcgatctcg atcattcgcg caacgccgtg agacgacggg tgtcgagcgt atcaaactcg
     2521 cgctggattc gatccacgat gatgccgacc acgaataccg cgacggtgag gtcgagcagg
     2581 atcccggctt cgaccatgat cggcgtcgag ccgatgagca gaagaccgaa catgtagatg
     2641 ccgttctcga gcatgagata gccgcagacc tgcgcgatcg ccttggcgcg ccccatcaag
     2701 agcagaaagc cggacacgac ggtcgcgaag gcgcccggga cgatcagcga gtgcgcgtgc
     2761 tcggctcgca gcggcagtac gccgccgagt gccacggctc caacggtcgc cgccgccccg
     2821 agcaagagcg aggggacgaa tggaatgagg ggttgtactt cgcggtcgat gtgggccgag
     2881 cgaagtgcct tgcgcaacag ccccggtatc aggaatccct tcacggccac ggtcgccgcc
     2941 gcgatcagaa tgacccacca attcgcccca tggccgagca gcagcgggag cactccgagc
     3001 accatcccct gaacggccgc cgcccggatg agcgacgtca gtcggctgct ggccaggccg
     3061 atcaagttca gccccatggc gaccccgacg aggaggttgt ggacgtcgtt cactgcggac
     3121 tccgttccca cgcgatcatg agcgcaaaga ggcaaagcaa gaacgccgtc gtcaggagca
     3181 tcggcacgcg gcgaaacggc agtcgggctt gcatcgattc gaccacgccg acgctcaccg
     3241 cggtaaggag cacgccgccg ccgagcgtca ccgccacgac cgcgggcgac agacccccga
     3301 gcggaaccac ggcctccgtc agcaagacgg agaatatcag g
//
LOCUS       DASPGK010000138         6430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1558208, whole genome shotgun sequence.
ACCESSION   DASPGK010000138 DASPGK010000000
VERSION     DASPGK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558208"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1435)
                     /gene="lat"
                     /locus_tag="VEC56_03975"
     CDS             complement(92..1435)
                     /gene="lat"
                     /locus_tag="VEC56_03975"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HXV13344.1"
                     /translation="MAHATGAGTDIHVEPKKVLETIGRHMLVDGFPIVVDLENSEGIR
                     LRDKADGRTYLDFFQFFASLPIGINHPKLTDPAFRERLGALAVNKPSNSDFYSQQMAE
                     FVEAMDRFAIPKELPHMFLIEGGALGIENALKAAFDWKVQKNYKRGATKDIGQQVIHF
                     REAFHGRTGYTMSLTNTDPVKIKHYPKFDWPRVVNPKVTFPLERHLEQVVAFEEQAKK
                     EIKEAIRVHGDDIAALIIESIQGEGGDNHFRPEFFRFLREVTLEHDIMFIVDEVQSGV
                     GITGKFWGYEHNGIVPDMITFGKKLQVCGFLCSTRIDEVDSVFKVPSRINSTWGGNLV
                     DMVRATRYLQVIEEEGLVENSRVVGDYLLGKLHELRDKYAGKVTNTRGRGVMNAFDLP
                     DPETRSKYLAKAKENGVLMVGCGTRSVRFRPPLNLKKSEVDEGVAIIDRSLGQVL"
     gene            complement(1609..3207)
                     /locus_tag="VEC56_03980"
     CDS             complement(1609..3207)
                     /locus_tag="VEC56_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV13345.1"
                     /translation="MPTLVIAGARVWTDDGAGSIRFEPRSIWIRDGRIVEVLPGVAAP
                     QPGARPDLHVFDATGKFVGPAFVDAHFHLIALANKALRVDLSAATDARDVARRLAGAG
                     GAEAGPPRPRTRGGEATGPAASRDAIVGVDFDESEWRDATLPTRADLNAVSGTRLVYA
                     RRVCCHVGVANDALLRLLEPRVAERFVDRESGRITEDAVFEANRLTRPPDDAVAAAMD
                     GAIAHLHSLGITAIHDIVDPDTIEVYGAGLRASRRPLRIDAYLHLPARDFEPARARFD
                     AIDPRRARAVGIKIFSDGSMGARTAALHEPYADAQTTGDLLVDRRALAAELAACARGG
                     FACAVHAIGDRAFSTVLDAIADSPGARVRIEHAEVIREAEIERAARAGIPLVMQPNFV
                     RNWGGEGGLYERRLGRQRWERNNPFATLLRAGARVVFSSDGMPAGPLYGLKGATHHAV
                     ERERVGAVDAFRRYTTAASMLWNANEGGPTIEAGAPADLLVLSGNPLFSDTDRLGVEG
                     TFAEGVEVYRSPAADPPRHSKGAR"
     gene            complement(3316..3696)
                     /locus_tag="VEC56_03985"
     CDS             complement(3316..3696)
                     /locus_tag="VEC56_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13346.1"
                     /translation="MRKFVWTFALSLLIAAPAFADAVYYAPQVTGKKERKFLRHYLYI
                     KNLDGDKKAVYDEYGYTAHRVRLNEYGRVKEKWTYYERGLVFVFDQCNELLETHEIEP
                     EKRRTWQYQRDVAGYDEDVCCDDE"
     gene            complement(4053..>6430)
                     /locus_tag="VEC56_03990"
     CDS             complement(4053..>6430)
                     /locus_tag="VEC56_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13347.1"
                     /translation="RAARSAEGYCHMKQGIAFVLACTALLAACASDEIGGMAAENQPP
                     TVWIAAAPPEGSTASYSVKIYWGGWDPDGEISHYEYFITDNVRGSFIPADTVDADWNP
                     VLSNDSTFTFSADSLAEPGPGQQAAVFTRSHTFFIRAVDREGLRSREPAYRSFTARTI
                     SPEVTIDTPRQALGLTPSELPPIATYQWTAVDYIDDMITRQQPESVQWALESTVNHSA
                     SFTATLDYLRSPASAASWYPWIYYRAPQDSGKSWTTPPMEFGDYVFAIRAKDEAGAVT
                     PVLDERRNARRIKVSRRTTGPRLVVSNQYVGSVVASTCDYPLTILDMVAGVGMAFTLS
                     ACADDYGGTVSGYRYGWDILDLTDPDQWETDYTPFVGSLAEIPARSFNFGTHTFTAEV
                     IDNSGFCSRIEVKVNIVRFSGERNLLLVDDYGADENPGFSGFDLTNGSVPNDAEHDAF
                     WRDMVSNVADFDPSRDVISVSAEREIPLTTLAAYKAIVWSVFSDLTTTNVARLPLLYQ
                     YIQFRGTQAQSGTSACNAGAGVSGKVVPNALALAMQAGAHVLIAGQQPVQNVLPRRSD
                     LLVRWPMIPLYETEDPQTGAPDVSDPPGENGFAYKDLCLEAIDYGYLPTSRARLPGSG
                     STRRYCPVSPVYRQPGSASTRDDTMREAQPLDPGFPPLRLRPEVAGAGRVYAPESQGI
                     DAEVYNPSYFRTGLACQYVPEPRPCFEPIYGLVSRDTNERTYLQPIAFWTSVYGDVVA
                     QDIPGAVAARSAVFGFPPVYFNPHEVKPAIEYILFDEWQLPRIAGEVSGSR"
BASE COUNT         1098 a         2066 c         2156 g         1110 t
ORIGIN      
        1 cgagagatcg cagtatcgta tcgtattcgc gccgcagaaa tcgcgttcga gggatcggtc
       61 gcatcggcct ctacctcatg gcagcgtcct gctaaagcac ctgtcccagc gagcggtcga
      121 tgatggcgac gccctcgtcg acttcggact tcttgagatt gagcggcggg cggaagcgaa
      181 ccgaccgcgt cccgcaaccc accatgagca ctccgttctc cttggccttg gcgaggtact
      241 tggagcgcgt ctcggggtcg ggcaggtcga aagcgttcat gacgccccgt ccgcgcgtgt
      301 tcgtcacctt gccggcgtac ttgtcgcgca gttcgtggag cttgcccagg agataatcgc
      361 cgaccacgcg cgagttctcg accagcccct cctcctcgat gacctgcagg tagcgcgtgg
      421 cgcgcaccat gtcgaccaga tttccgcccc aggtggagtt gatgcggctt ggcaccttga
      481 agacgctgtc gacttcgtcg atgcgcgtcg agcacaagaa cccgcacacc tggagcttct
      541 ttccgaaggt gatcatgtcg gggacgatgc cgttgtgctc gtagccccag aacttgcccg
      601 tgatgcccac gcccgactgg acttcgtcga cgatgaacat gatgtcgtgc tcgagggtga
      661 cctcgcgcaa gaagcggaag aactcggggc ggaaatgatt gtcgccgcct tcgccctgga
      721 tcgactcgat gatgagcgcc gcgatgtcgt cgccgtggac gcggatcgct tccttgattt
      781 ctttcttggc ctgttcctcg aaggccacga cctgctccag gtggcgctcg agcgggaagg
      841 tgaccttggg attgacgacg cgcggccagt cgaacttcgg gtagtgcttg atcttgaccg
      901 ggtcggtgtt ggtcagagac atggtgtagc cggtgcgacc gtggaaggct tcgcggaagt
      961 gaatcacctg ctggccgatg tctttggtgg ccccacgctt gtagttcttc tgcaccttcc
     1021 agtcgaaggc cgccttgagc gcgttctcga tgccgagcgc accgccttcg atcaggaaca
     1081 tgtgcggcag ctctttggga atcgcgaagc gatccatcgc ttccacgaac tcggccatct
     1141 gttggctata gaaatcggag ttcgacggct tgttgaccgc gagggcaccc aggcgctcgc
     1201 ggaacgcggg gtcggtcagc ttgggatgat tgatcccgat cggcagcgac gcgaagaatt
     1261 ggaagaagtc gaggtaggtg cggccgtccg ccttgtcgcg cagccgaatg ccctcgctgt
     1321 tttcgagatc gacgacgatc ggaaacccgt cgaccagcat gtggcggccg atggtctcga
     1381 gcaccttctt gggctcgaca tggatgtccg ttccggcccc agtcgcgtgc gccatggaac
     1441 tctcctcttg tgatgaagaa aaaaagcgac tcggtcgtgc gcgcggcccg tccccgcacc
     1501 caccgcagta acccgctgtc aggactaaag ctaagggatg gtacgagggg gtgtcaagaa
     1561 cgcaggatga cgccgacagg cgtccgcaac ccacgccatg ggtgcgagtt agcgagcacc
     1621 cttcgagtga cgcggtgggt ccgcggcggg tgaccgatag acctcgacgc cttcggcgaa
     1681 ggtgccctcg acaccaagcc gatcggtgtc cgaaaaaagc gggtttccgg agagaaccag
     1741 gaggtcggcg ggcgccccgg cttcgattgt gggcccgccc tcgttcgcgt tccagagcat
     1801 ggacgccgcc gtcgtgtagc gccggaacgc gtccacagca ccgacgcgct cgcgctcaac
     1861 cgcgtggtgt gtcgccccct tgagaccgta caagggcccc gccggcatgc cgtcggagct
     1921 gaacacgacc cgcgcgcccg cgcgcaacaa ggtcgcgaag gggttgttgc gttcccagcg
     1981 ctggcggccc agtcggcgct cgtacagacc gccttcgccg ccccaattgc gcacgaagtt
     2041 gggctgcatc acgagcggaa tcccggcgcg cgcggcccgc tcgatctccg cctcgcgaat
     2101 cacttccgcg tgctcgatcc ggacccgcgc gccgggagag tccgcgatcg catccagtac
     2161 ggtcgaaaag gcccggtccc cgatggcgtg gaccgcgcac gcgaacccgc cgcgcgcgca
     2221 cgcggctagc tcggccgcca gcgcgcgccg atccaccagg agatcgcccg tcgtctgcgc
     2281 gtccgcatac ggctcgtgga gcgcggctgt gcgcgcgccc atcgacccgt cggagaatat
     2341 cttgatgccg accgcgcgcg cgcgccgggg gtcaatggcg tcgaagcggg cgcgcgcggg
     2401 ctcgaagtcc cgcgcgggaa ggtgcaggta ggcgtcgatg cgcagcgggc gtcgcgacgc
     2461 gcgcagcccc gccccgtaaa cctcgatggt gtcgggatcg acgatgtcgt ggatggcggt
     2521 gatcccgagc gagtgcaggt gggcgatggc gccgtccatc gcggcggcca ccgcgtcgtc
     2581 gggcgggcgc gtgagccggt tggcctcgaa cacggcatcc tcggtgatgc ggccgctctc
     2641 gcggtcgacg aagcgctcgg caacacgcgg ctcgagcaac cgcagcaacg cgtcgttggc
     2701 gacaccgacg tggcagcaca cgcgccgcgc gtagaccagc cgggtcccgc tgacggcgtt
     2761 caagtcggcg cgcgtgggca gggtcgcgtc ccgccactcg ctctcgtcga agtcgacccc
     2821 gacgatggca tctctgctcg ccgcgggtcc tgtcgcctcg ccaccccgcg tccgaggacg
     2881 cgggggcccc gcctcggcgc cacccgcgcc ggccaagcga cgggctacat cacgtgcgtc
     2941 tgtggcggcg ctcaggtcga cgcgcagcgc cttgttcgcg agcgcgatca ggtggaagtg
     3001 cgcatcgacg aaggccgggc ccacgaattt cccggtcgcg tcgaagacgt ggaggtcggg
     3061 acgcgcgccg ggctgcgggg ccgcgacacc cgggaggact tcgacgatgc gcccgtcacg
     3121 gatccagatc gagcgcggct cgaagcgaat cgatcccgcg ccgtcgtcgg tccacacccg
     3181 ggcgcccgca atcaccagtg tcggcatggg gtgcatagta gcaaaaaaaa caagggggcc
     3241 cgattgttgg aggaggcccc cttgtcccaa cccgtccagt cgcggaccga acgggcagtc
     3301 atcgccgcac gcgatttatt cgtcgtcgca acagacgtct tcgtcgtacc ccgcgacgtc
     3361 gcgctggtac tgccacgtgc gccgcttctc gggttcgatc tcgtgcgtct cgagcagctc
     3421 gttgcactgg tcgaacacga agacgagtcc gcgctcgtag tacgtccact tctctttcac
     3481 acgtccgtac tcgttcaagc ggacgcgatg ggccgtgtag ccgtactcgt cgtagacggc
     3541 cttcttatcc ccgtcgaggt ttttgatata caaatagtga cggaggaact tgcgttcctt
     3601 cttccccgtc acctgaggcg cgtagtaaac ggcgtccgcg aaggcgggcg ctgcgatcag
     3661 cagcgacagg gcgaacgtcc acacgaactt tctcatactc ccactccttg atgctaacgc
     3721 tagtgatgct tggagcttac ggttgtgact tattgtatca catacagtct cgaaatggca
     3781 agatgggagg ccctgtggag gcttgcggcg ggaaacgccg acggatggat gggggcggct
     3841 taaccggctg ctagacggtc aattagtcgt gtctttgggg gcctggcgca gaggggaaaa
     3901 aagtagggcc cgctcgccgg ctgtcgcttg agtttggaac aggggcaaca ggcggaagac
     3961 aagcagggcc ctattatgtc gaagtcgaaa cgaagaccgc gcgcagtata ccacgcaacc
     4021 gaagctggcg cacccccggg attccgggct cgctaccgcg agcccgagac ctcgcccgcg
     4081 atccgcggga gctgccactc gtcgaacaga atgtactcaa tggccggctt cacttcgtgc
     4141 gggttgaagt agaccggcgg gaagccgaag accgcgctgc gcgcggccac cgcgcccggg
     4201 atatcctgtg ccaccacgtc gccgtacacg ctggtccaga acgcgatcgg ctgcaagtag
     4261 gtccgctcgt tggtgtcccg gctgacgagc ccgtagatcg gctcgaaaca cggacgcggc
     4321 tccggcacgt actggcacgc cagcccggtg cggaagtacg acggattgta cacctcggcg
     4381 tcgatgccct gcgactcggg cgcgtacacg cgccccgctc ccgcgacctc gggtcgcagg
     4441 cgcagcggcg ggaagccggg gtcgagcggc tgcgcttcgc gcatcgtgtc gtcgcgggtg
     4501 gacgccgagc ccggctgccg atagacggga ctcaccgggc aatagcgccg cgtgcttccg
     4561 gatcccggca agcgcgcgcg cgatgtcggg aggtagccgt aatcgatcgc ctccaggcac
     4621 agatccttgt acgcgaatcc gttctcaccc ggcgggtcgg acacgtcggg ggcgccggtc
     4681 tgcggatcct cggtctcgta gagcggtatc atcggccaac gcaccaggag atccgagcgg
     4741 cgcggcagca cgttctgaac cggctgctga cccgcgatca gcacgtgggc gcccgcctgc
     4801 atcgcgagtg ccaaggcatt gggcacgacc ttgcccgaca ccccggcacc ggcgttgcac
     4861 gccgaggtcc ccgactgcgc ctgcgtgccg cggaattgga tgtactggta taggagcggc
     4921 aggcgagcga cgttggtggt ggtcagatcg ctgaacacgc tccacacgat ggccttgtac
     4981 gccgccagcg tggtgagcgg gatctcgcgc tcggcgctga ccgaaatcac gtcgcgcgaa
     5041 gggtcgaagt cggccacgtt cgataccatg tcgcgccaga aggcgtcgtg ctcggcgtcg
     5101 ttgggcacgc tcccgttggt caggtcgaat cccgagaatc cggggttctc atcggcgccg
     5161 tagtcgtcga cgagcaacag gttacgctcg ccgctgaagc ggacgatgtt caccttcact
     5221 tcgatgcgcg agcagaagcc cgagttgtcg atcacctcgg ccgtgaaggt atgggtgccg
     5281 aagttgaagg aacgggccgg gatctcggcg agcgaaccca cgaacggcgt gtagtccgtc
     5341 tcccactggt ccggatcggt tagatcgagg atatcccagc cgtaacgata ccccgacacg
     5401 gtgccgccgt agtcgtctgc gcacgcgctc agagtgaacg ccatgcctac gccggcgacc
     5461 atgtcgagga tggtcagcgg gtagtcgcac gttgaagcta ccacgctgcc cacgtactga
     5521 ttgctgacga cgagccgcgg gccggtcgtc cgacgcgata ccttgatgcg acgcgcgttc
     5581 cggcgctcgt cgagcacggg cgtcaccgca ccggcctcgt ccttggcgcg aatcgcgaac
     5641 acgtagtcgc cgaactccat cggtggagtg gtccacgact taccgctgtc ttggggagcc
     5701 cggtaataga tccacgggta ccacgacgcc gccgaggccg ggctgcgcaa gtaatcgagc
     5761 gtggcggtga agctggcgct gtggttgacg gtgctctcga gggcccattg cacggactcc
     5821 ggctgctggc gcgtgatcat gtcgtcgatg tagtcaaccg cggtccactg ataggtggcg
     5881 atgggaggaa gctccgacgg cgtcagtccc agggcctggc gcggcgtgtc gatggtgacc
     5941 tcgggcgaga tcgtgcgcgc ggtaaacgaa cggtacgcgg gctcgcgcga ccggagcccc
     6001 tcgcgatcga cggcccgaat aaagaacgtg tgagaccgcg tgaacaccgc cgcctgctgc
     6061 ccggggccgg gctccgcgag cgaatcggcg gaaaatgtga atgtggagtc gttggacagc
     6121 accgggttcc agtccgcgtc caccgtgtcc gcggggatga acgagcccct cacgttatcg
     6181 gtgatgaagt actcgtagtg agagatctcg ccgtcggggt cccatccgcc ccaatagatc
     6241 ttgaccgagt agctggcggt cgagccttcg ggaggcgccg ccgcgatcca aaccgtgggg
     6301 ggttgatttt ccgccgccat gcctccgatt tcgtcggatg cgcaggcggc gaggagagct
     6361 gtgcacgcga gaacgaacgc gatgccttgc ttcatgtgac agtacccttc cgcactccgc
     6421 gccgcgcggg
//
LOCUS       DASPGK010000139         5673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_387214, whole genome shotgun sequence.
ACCESSION   DASPGK010000139 DASPGK010000000
VERSION     DASPGK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387214"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..644
                     /gene="rplD"
                     /locus_tag="VEC56_03995"
     CDS             12..644
                     /gene="rplD"
                     /locus_tag="VEC56_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXV13348.1"
                     /translation="MAKANCFEKDGSRAGTVDLPKSIFEVEVNLFAVHEAVVAYLANQ
                     RQGTVNTKERSDVSGGGKKPWKQKGTGRARAGTIRSPLWRGGGTVFGPHPRDHRLKLN
                     KKVKRVALKSSLTSRALEGNVLVIKSLAFDAPKTKTFAAVLKNMEAYPRKKTLVVLDK
                     ASEATLKSARNVAGVRLTAADKVNTYDVVWADKIVVTTDALRRMEEVYAS"
     gene            641..949
                     /gene="rplW"
                     /locus_tag="VEC56_04000"
     CDS             641..949
                     /gene="rplW"
                     /locus_tag="VEC56_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXV13349.1"
                     /translation="MRERDIRFVIERPMLTEKGAEMKELKNRYVFRVNRKANKREIKE
                     AVEKMFNVKVKDVRTAVYGGKKSVAMNRSGRYSGLRSSWKKAYVTLAEGQNIEAFDLV
                     "
     gene            962..1804
                     /gene="rplB"
                     /locus_tag="VEC56_04005"
     CDS             962..1804
                     /gene="rplB"
                     /locus_tag="VEC56_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXV13350.1"
                     /translation="MGIKKFNPITPTLRYRTVSDFAEITKSTPEKSLTESLGKTGGRN
                     NVGRQTSRRRGGGHKRLYRVVDFKRKKRGIPASVVAIEYDPNRTSRIALVHYADGEKS
                     YIIAPNDLKVGDRIVAGPEADIKPGNALPLEKVPVGTMVHNVELVAGKGGQMVRSAGS
                     FAQLMAKEGDYATLRLPSGEMRMVRKECYASVGQVGNLDHENVVSGKAGRTRWLGKRP
                     QVRGVAMNPVDHPLGGGEGKSSGGRPGCSPWGLPDGRKTRKRKKPSSRLIVRRRVNKL
                     TEGK"
     gene            1814..2110
                     /gene="rpsS"
                     /locus_tag="VEC56_04010"
     CDS             1814..2110
                     /gene="rpsS"
                     /locus_tag="VEC56_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXV13351.1"
                     /translation="MPRSTKKGPYIDENLLGRITSLNQTGEKRVVKTWARRSTISPEM
                     VGHTLAVHNGKQFIPVYVTENMVGHKVGEFSLTRTFRGHTFKTKEEKAAKKPGA"
     gene            2141..2500
                     /gene="rplV"
                     /locus_tag="VEC56_04015"
     CDS             2141..2500
                     /gene="rplV"
                     /locus_tag="VEC56_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXV13352.1"
                     /translation="MMDAFAVSKHVGYPPRKVRQLADMVRGQKVDSALTTLRFTKRAA
                     SAAVRKTIQSAMHNLVVKHGGSVSAEDMVVKSILIDEGRTMYRIRPRAQGRAYRIRKR
                     SSHIKVTVTLLEEKKNA"
     gene            2528..3199
                     /gene="rpsC"
                     /locus_tag="VEC56_04020"
     CDS             2528..3199
                     /gene="rpsC"
                     /locus_tag="VEC56_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXV13353.1"
                     /translation="MGQKTNPVGLRLGINRTWDSRWFSSKEYSKWLDEDIKVRNYVKR
                     RLFKAGVARIEIERSGGKATVNIYTARPGMVIGRKGSEVDQLREELKQRCGREVQINI
                     KDVKKPELNAQLVAEHIALQLQQRVAFRRAMKKTIASAMRMGAQGIKVQCSGRLGGAE
                     IARREGYHEGRVPLHTLRADIDFARWTAHTTFGCVGVKVWIYKGEIFPDEFKKNLAKA
                     AEGGR"
     gene            3202..3618
                     /gene="rplP"
                     /locus_tag="VEC56_04025"
     CDS             3202..3618
                     /gene="rplP"
                     /locus_tag="VEC56_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXV13354.1"
                     /translation="MLMPKRVKHRKQQRGRMGGQATRGATIAFGEYGLKAMEPQWITN
                     RQIEAARVAITRHIKRVGKLWIRVFPDKPVTVKPAETRMGKGKGNPEYWVAVVKPGRV
                     LFELEGVPADVARRALELAASKLPCKTKFVQRNVEE"
     gene            3621..3818
                     /gene="rpmC"
                     /locus_tag="VEC56_04030"
     CDS             3621..3818
                     /gene="rpmC"
                     /locus_tag="VEC56_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXV13355.1"
                     /translation="MKPLEMRNMTVDELKAHHDTLIDELSGLRLKHVLRQLDDPMKVR
                     ALRRQIARAKTIIRQKQTSAK"
     gene            3849..4124
                     /gene="rpsQ"
                     /locus_tag="VEC56_04035"
     CDS             3849..4124
                     /gene="rpsQ"
                     /locus_tag="VEC56_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXV13356.1"
                     /translation="MTETKTATRPEGRKTFVGVVHSNKMNKTIVVTVERLVKHAQYGK
                     YIKRTTKLYAHDEENKAGIGDKVLVMSTRPLSKTKRWRLIEVLERAK"
     gene            4162..4530
                     /gene="rplN"
                     /locus_tag="VEC56_04040"
     CDS             4162..4530
                     /gene="rplN"
                     /locus_tag="VEC56_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXV13357.1"
                     /translation="MIKMRTMLQVADNSGAKSLQCIQVVGSTKKNSAAIGDIISVSVK
                     MAIPGGMVKKGQVAKAVVVRTKKETRREDGTFIRFDENAAVIINDAKEPIGTRIFGPV
                     ARELREKKFMKIISLAPEVL"
     gene            4544..4864
                     /gene="rplX"
                     /locus_tag="VEC56_04045"
     CDS             4544..4864
                     /gene="rplX"
                     /locus_tag="VEC56_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXV13358.1"
                     /translation="MRSIVKNDLVKVISGNYKGKTGKVIKVLVDKDRVIVEKIRMVKR
                     HTKPRSAQQPGGIVEKEASVHISSVMLVCPKCGKPTRTRTGALANGKKVRVCGACEEM
                     ITGE"
     gene            4867..5409
                     /gene="rplE"
                     /locus_tag="VEC56_04050"
     CDS             4867..5409
                     /gene="rplE"
                     /locus_tag="VEC56_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXV13359.1"
                     /translation="MARLKEFYNKEVVPALKKDFGFTNPMEVPHLKKIVVNMGVGEAV
                     ANGKLVDAAMSDLGLITGQKPQLNRARISVAAFKLREGMPIGCKVTLRGERMYEFFDR
                     LVNLSLPRIRDFRGVPTKGFDGRGNYTLGVKEHIIFPEIDYDKIAQVFGMDITIVTSA
                     RTDEQALALLTHMKMPFRKN"
     gene            5440..5625
                     /locus_tag="VEC56_04055"
     CDS             5440..5625
                     /locus_tag="VEC56_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011077818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXV13360.1"
                     /translation="MAKKSWIAQTKRPPKFKVRAYSRCNRCGRSRAYLRRFGICRICF
                     RDLALQGEVPGVVKASW"
BASE COUNT         1238 a         1671 c         1910 g          854 t
ORIGIN      
        1 caggggtcaa tatggcgaag gcaaattgct tcgaaaaaga cggatcgcgc gcgggcacgg
       61 tcgacctccc gaagagcatc ttcgaggtcg aggtcaacct gttcgccgtg cacgaggccg
      121 tggtcgcgta cctcgcgaac cagcggcagg gaaccgtcaa cacgaaggag cgctcggacg
      181 tgagcggcgg cggcaagaag ccctggaagc agaagggaac cggccgtgcg cgtgccggca
      241 ccattcgctc gccgctgtgg cgcggcggcg gcaccgtgtt cggaccgcac ccgcgcgacc
      301 accgcttgaa actcaacaag aaggtcaagc gcgtggcgct caagtcgtcg ctgaccagcc
      361 gcgccctcga gggcaacgtg ttggtgatca agtcgctcgc gttcgacgcg cccaagacca
      421 agactttcgc ggccgtgttg aagaacatgg aagcctaccc gcgcaagaag acgctggtcg
      481 tgctcgacaa ggccagcgag gcaaccttga agtcggcccg caatgtggcc ggcgtgcgct
      541 tgaccgcggc cgacaaggtc aatacctacg acgtcgtgtg ggccgacaag atcgtcgtca
      601 ccaccgacgc gctgcgccgc atggaagagg tgtacgcgtc atgagagagc gcgacattcg
      661 tttcgtgatc gagcgcccca tgctcaccga aaagggcgcc gagatgaaag agctcaagaa
      721 ccgctacgtg tttcgcgtca accgcaaggc caacaagcgc gagatcaagg aagcggtgga
      781 gaagatgttc aacgtgaagg tcaaggacgt gcgcaccgcc gtatacggcg gcaagaaatc
      841 ggtcgcgatg aaccgctccg gtcgctacag cggattgcgt tcgtcgtgga agaaagcgta
      901 cgtgacgctg gccgagggtc agaacatcga ggccttcgac ctcgtctagg gaaggatcgc
      961 gatgggtatc aagaagttca atccgatcac gccgacgctg cgctaccgca ccgtctccga
     1021 cttcgcggaa atcacgaagt cgacgccgga gaagtcgctg accgagtcgc tcggcaagac
     1081 cggcggccgc aataacgtgg ggcgccagac ctcgcgtcgc cgcggcggcg gccacaagcg
     1141 cctttaccgc gtcgtcgatt tcaagcgcaa gaagcgcggg attccggcgt cggtggtggc
     1201 gatcgagtac gatccgaatc gcacctcgcg catcgcgctc gtccactacg cggacggcga
     1261 gaagagctac atcatcgcgc ccaacgacct caaggtgggc gaccggatcg tcgcgggtcc
     1321 cgaagccgac atcaagccgg gcaacgcact cccgctcgag aaggtgccgg tcggcaccat
     1381 ggtgcacaat gtcgagctcg tggccggcaa gggcggacag atggtgcgct cggccggatc
     1441 cttcgcgcag ttgatggcga aggagggcga ctacgcgacg ctgcgtttgc cgtccggcga
     1501 gatgcggatg gtgcgcaagg agtgctacgc gagcgtgggc caggtcggga atctcgacca
     1561 cgagaacgta gtctccggca aggccggccg cacgcggtgg ctgggcaagc gtccgcaggt
     1621 gcgcggcgtc gcgatgaacc ccgtcgatca cccgttgggc ggcggcgagg gcaagtcgag
     1681 cggcggtcgc ccgggctgca gtccgtgggg cctccccgac ggccgcaaga cgcgcaagcg
     1741 gaagaaaccg agctcgcgac tgatcgttcg tcgtcgcgtg aacaagttga ccgaaggaaa
     1801 gtaagggtcg gacatgccga gaagcacaaa gaagggcccg tacatcgacg agaacctgct
     1861 gggcaggata acgtcgctca atcagacggg cgagaagcgc gtggtcaaga cctgggcgcg
     1921 tcgttcgacg atttcgcccg agatggtggg ccacacgctc gcggttcaca acggcaagca
     1981 gttcatcccg gtctacgtga ccgagaacat ggtggggcac aaagtcggcg agttttcgct
     2041 gacgcggacg ttccgcggcc acaccttcaa gaccaaggaa gaaaaggccg cgaagaagcc
     2101 gggcgcgtag ccccgccctc tcgcgacgtt caaggacggc atgatggacg cgttcgcagt
     2161 ttccaagcac gttggctacc cgccccgcaa ggtgcgtcag ctcgccgaca tggtgcgagg
     2221 gcagaaggtg gatagcgcgc tcacgacgct tcgcttcacc aagcgcgcgg cgtcggcggc
     2281 ggtgcgcaag acgatccaat ccgcgatgca caacctcgtg gtcaagcacg gcggttcggt
     2341 gtcggcggaa gacatggtcg tcaagtcgat cctcatcgac gaggggcgca cgatgtaccg
     2401 gattcgcccg cgcgcgcagg gtcgcgccta tcggattcgc aagcgcagca gccacatcaa
     2461 ggtgaccgtg accctcctgg aggagaaaaa gaatgcgtga gtcgacggta gtgcggaggt
     2521 ccgagtaatg ggacagaaga ccaatccggt cgggctgcgg ctcgggatca accgcacgtg
     2581 ggattcccgg tggttctcct cgaaggagta ctcgaagtgg ctcgacgagg acatcaaggt
     2641 gcgcaactac gtcaagcgcc ggttgttcaa ggccggtgtc gctcgcatcg agatcgagcg
     2701 ctcgggcggt aaggcgaccg tcaacatcta taccgcgcgt ccgggcatgg tgatcggccg
     2761 caagggctcc gaggtcgacc agctgcgcga agaactgaag cagcgctgcg gccgcgaggt
     2821 ccagatcaat atcaaggacg tcaagaagcc ggagctcaac gcgcagctgg tcgccgagca
     2881 catcgcgctg cagttgcagc agcgcgtcgc gttccgtcgc gccatgaaga agacgatcgc
     2941 gtcggccatg cgcatgggcg cgcagggcat caaggtgcag tgctcgggcc gcctgggcgg
     3001 cgccgagatc gcgcgccgcg agggttacca cgagggtcgc gtgccgctgc acacgttgcg
     3061 cgcggacatc gatttcgcgc gctggaccgc gcacaccacc ttcgggtgcg tgggcgtcaa
     3121 ggtgtggatc tacaagggag agatcttccc cgacgagttc aaaaagaacc tcgccaaagc
     3181 ggccgaggga ggacgttagc catgttgatg ccaaagcgcg taaagcaccg gaagcagcag
     3241 cgcggccgca tgggcggcca ggccacgcgc ggcgccacca ttgcattcgg cgagtacggc
     3301 ttgaaggcga tggagccaca gtggatcacc aaccgccaga tcgaagccgc tcgcgtcgcg
     3361 attacgcgcc acatcaagcg cgtgggcaag ctgtggattc gcgtgtttcc cgacaagccg
     3421 gtcacggtga agccggccga gacgcgcatg ggtaaaggaa agggcaaccc cgagtactgg
     3481 gtcgccgtcg tgaagccggg tcgcgtgttg ttcgagctcg aaggcgtacc cgccgacgtc
     3541 gctcgccgcg cgctcgagct ggcggcgtcc aagctcccgt gcaagaccaa gttcgtgcag
     3601 cgcaacgtgg aggaataggg atgaagccgc tcgagatgcg aaacatgacc gtcgacgagc
     3661 tcaaggctca ccacgacacg ctgatcgatg agctgtcggg gctgcgcctc aagcacgtgc
     3721 tgcgccagct cgacgatccc atgaaggtgc gcgcgttgcg caggcagatt gcgcgcgcca
     3781 agacgatcat tcggcagaag cagacgagcg ccaagtaggc cctcgcgcgg atgcaagcag
     3841 gtatatcgat gaccgaaacg aaaacagcga cgaggccgga aggacgcaag accttcgtcg
     3901 gcgtcgttca tagcaacaag atgaacaaga ccatcgtcgt gacggtggag cgcctggtga
     3961 agcacgcgca gtacggtaag tacatcaagc gcaccaccaa gctctatgcg cacgacgagg
     4021 agaacaaggc cggcatcggg gacaaggtgc tcgtcatgtc gacgcgcccc ctgagcaaga
     4081 ccaagcgctg gcggctgatc gaagtgctcg aacgggcgaa gtgagccgtt cgctctagaa
     4141 acggaacgcg gtaggtaaac catgatcaag atgcggacaa tgcttcaggt cgccgacaac
     4201 tcgggtgcga agagtctgca atgcatccaa gtggtcggtt cgacgaagaa gaacagcgcc
     4261 gctatcggcg acatcatctc ggtttcggtc aagatggcga tcccgggggg aatggtgaag
     4321 aaggggcagg tggccaaggc ggtcgtggtt cgcaccaaga aggagacgcg ccgcgaggac
     4381 gggacgttca tccgtttcga cgagaacgcg gccgtcatca tcaacgacgc caaggagccc
     4441 atcgggaccc gaatcttcgg cccggtggcg cgcgaacttc gcgagaagaa gttcatgaag
     4501 atcatctcgc tcgcacccga agtgctgtga gcgcgggaga accatgagaa gcatcgtaaa
     4561 gaacgatttg gtgaaggtga tttccggcaa ctacaagggc aagaccggaa aagtcatcaa
     4621 ggtgctggtc gacaaggacc gcgtgatcgt cgagaagatc cgcatggtca agcggcacac
     4681 caagccgcgg tcggcgcagc agccgggcgg catcgtcgag aaggaagcgt cggtccacat
     4741 ctccagcgtc atgctggtgt gcccgaagtg cggaaagccg acgcgcacgc gcacaggcgc
     4801 gctcgccaac ggcaagaagg tccgcgtgtg cggggcgtgc gaagaaatga tcacgggtga
     4861 gtagcaatgg ctagactcaa agagttctat aacaaggaag tcgttccggc cctcaagaag
     4921 gactttgggt tcaccaaccc gatggaagtc ccgcatctga agaagatcgt ggtcaacatg
     4981 ggcgtgggcg aagcggttgc caacggcaag ctggtcgacg ccgccatgag cgacctcggc
     5041 ctaatcaccg ggcagaagcc gcagctgaat cgcgcgcgca tctcggtcgc ggccttcaag
     5101 ctgcgcgagg gcatgcccat cggctgcaag gtgacgctgc gcggcgagcg catgtacgag
     5161 tttttcgacc ggctggtcaa tctgtcgctc ccgcgcatcc gcgactttcg cggcgtgccg
     5221 accaagggct tcgacgggcg cggtaactac acgctgggtg tgaaggaaca catcattttc
     5281 ccggagatcg actacgacaa gatcgcgcag gtcttcggga tggacatcac gatcgtgacg
     5341 tcggcgcgaa ccgacgagca ggcgttggcg ctcctgacgc acatgaagat gccgttccgc
     5401 aagaactagg cggacggcga gtcgtttaga aaggtaaacg tggcaaagaa atcctggatc
     5461 gcgcagacca agcggccgcc gaagttcaag gtgcgcgcgt acagccgttg caaccgctgc
     5521 ggtcgttcgc gtgcgtacct gcgccgcttc ggcatctgcc gaatttgctt ccgcgacctc
     5581 gccctgcagg gtgaagtccc gggtgtcgtc aaggctagct ggtagaagca caagcggtcc
     5641 gagtcgcgtc aacagcgttc gacgcggact tcc
//
LOCUS       DASPGK010000140         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_387571, whole genome shotgun sequence.
ACCESSION   DASPGK010000140 DASPGK010000000
VERSION     DASPGK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387571"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEC56_04060"
     CDS             complement(<1..439)
                     /locus_tag="VEC56_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13361.1"
                     /translation="MKKFIFILIFFVSVGVSYVIWDNSPEYLKQGGPLLVVGLTLLFL
                     VFTFTVERAFVLWRAGGKGGVSTFMRNVKSAVQENDIDRAVEACRKQGGSLGNVVGAG
                     LERYNMERTKLDSPKDLLEETKHAIEEATALEAPILERNLSALS"
     gene            complement(587..2401)
                     /locus_tag="VEC56_04065"
     CDS             complement(587..2401)
                     /locus_tag="VEC56_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13362.1"
                     /translation="MSLLRHVAIAAFVVPCLATSVSWAQKSYTYETDPVRVGTKALEK
                     NDLAAAKAAFDEAVTNSYELPKAKVGLAEVATRSGRFEEAENLYREALDIQGRESGKG
                     FPEADAGLGILLIDADRWDEGVTHINKAYKQNSGYWPAIYGQARIEIRNKKWDKAKSY
                     LDWGASRKGVGQGEDLYHRGMALYFMGRNDLQGAEKEALTALHMNPTDPRHGVLVAQV
                     YEKRNVPASAIAACEEVLRTPGVTPTASFIHFLGTLYQKAGRYNDARDKYVQSVDMDS
                     TYAPALKDLGGLFVLAKQYDNASKVYLRYIELEPDDIEAYVGLTESLGESGRYAQALQ
                     SANKAMAIDSTRADVRLVYARTALRNRDKALRSRGAEMYASLPDSVSLGAEDLVLLAS
                     YEIDSGSLEEAKTHLDQAVAADSSNAEAHFQRGLLAFKNSQPDQAVGHFEEAIKRDPR
                     VSLYHLNLGVAYFQANQIDKAKPELRRAIALDKRPVMGHVLLGQALVSSDSLPAAEAE
                     YQKALTLDPKNARALRGLGFTYLRTASFDKAAQSYRAATDADPRNADGWAGLGQSYLG
                     LGDLASAEDAFSKAQSIDPSNPSLKRGRDLLNKARKGG"
     gene            complement(2422..>3237)
                     /locus_tag="VEC56_04070"
     CDS             complement(2422..>3237)
                     /locus_tag="VEC56_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXV13363.1"
                     /translation="DLMPQLIKKYHDIYPKLHVRLEDGGTVHALEHLANGRAQVGFLY
                     RLPTPDERRLVRIAVDDTVLCFPIALGGVAVLANSLSGVESFAMEELRAIARDQADPR
                     FDRLYAPDPNQGLWDAFRASLAVTEDVARPGGNAAPELAHITFLADEAAVIEAVLADP
                     RGLGLASTLSMPDSLPDMGGGRSVRAVPVLPDTGLVPVEPGYEQIGYGEYPLYHYLYV
                     ACLAGGSIRGSMFVTHLTSDRGQRHVERAGFLPARQSIRQIYLTRKPLGSKAN"
BASE COUNT          513 a         1066 c         1060 g          598 t
ORIGIN      
        1 tcgacagcgc gctcaggtta cgctccagaa tgggcgcttc gagcgcggtg gcctcttcga
       61 tggcgtgctt ggtctcttcg agcaaatcct tggggctgtc gagcttggtg cgttccatgt
      121 tgtagcgctc gaggcctgcg cccaccacgt tgcccaggct gccgccctgt ttgcggcacg
      181 cctcgacggc gcggtcgatg tcgttttcct ggaccgcgga cttgacgtta cgcatgaacg
      241 tcgacacgcc gcccttgccg cccgcgcgcc agagcacgaa cgcgcgctcg accgtgaacg
      301 tgaacacgag gaacaggagt gtcaaaccga cgacaagaag cgggccaccc tgcttcaagt
      361 actccggcga gttgtcccag atcacgtagg acacacccac ggagacgaag aaaatcagga
      421 tgaagatgaa ctttttcatt tttctccttc cgatgtccgg cgcgggtggg gcccgccctg
      481 aagctgcttc ggcgcgaagc ccgcgccggt gaaaggtgcc gcgcgggagt gaagcagttt
      541 acttacgttg acgactatcg tcaaacgaaa aacgggttta gcgctcctaa ccgcccttgc
      601 gggccttgtt caacagatcg cggccgcgct tgagcgaggg gttgctgggg tcgatcgact
      661 gcgccttgct gaaggcgtct tcggcgctcg caaggtcccc gagtcccagg tacgactggc
      721 ccagtccggc ccacccgtcg gcattgcgcg ggtcggcgtc ggtcgcggcc ctgtacgact
      781 gggccgcctt gtcgaacgac gccgtacgca agtaggtgaa gccgagcccg cgcagcgccc
      841 gggcgttctt gggatcgagc gtaagcgcct tctggtactc cgcttcggcc gcgggcagcg
      901 aatccgacga caccagggcc tgaccgagga gcacgtggcc catcaccggg cgcttgtcga
      961 gcgcgatcgc ccggcgcaac tccggcttgg ccttgtcgat ctggttggcc tggaagtagg
     1021 ccacgccgag attcaagtgg tacagcgaca ccctggggtc gcgcttgatc gcctcttcga
     1081 agtgaccgac ggcctggtcc ggctggctgt tcttgaacgc cagcaagccg cgctggaagt
     1141 gcgcctccgc gttcgacgag tccgccgcca ccgcctggtc gaggtgagtc ttcgcctctt
     1201 cgagactgcc gctgtcgatc tcgtagctcg ccagcagcac gaggtcctcc gcgcccagcg
     1261 acacgctgtc ggggagcgag gcgtacatct ccgcgccgcg cgagcgcaac gccttgtcgc
     1321 ggttgcgcag ggccgtgcgc gcgtacacga gccgcacgtc ggcgcgcgtg ctgtcgatcg
     1381 ccatggcttt gttggcggac tgcagcgcct gggcgtagcg gcccgactcg ccgagcgact
     1441 ccgtcaatcc gacgtacgct tcgatgtcgt ccggctcgag ctcgatgtag cgcaagtata
     1501 ccttggaagc gttgtcgtac tgcttggcga gcacgaacag gccccccagg tctttgagcg
     1561 cgggcgcata ggtcgaatcc atgtcgaccg actgcacgta cttgtcgcgc gcgtcgttgt
     1621 agcggcccgc cttctggtag agcgtgccca ggaagtggat gaacgacgcc gtcggcgtca
     1681 cacccggcgt gcgcaacacc tcctcgcagg ccgcgatggc ggacgcgggg acgttgcgct
     1741 tctcgtagac ctgggccacg agcacgccgt ggcgcgggtc ggtcgggttc atgtggaggg
     1801 ccgtcagcgc ttctttctcc gcgccttgca ggtcgttgcg ccccataaag tagagcgcca
     1861 tgcctcggtg atagaggtcc tcgccctgcc cgacgccctt acgcgacgcg ccccagtcga
     1921 ggtacgactt cgccttgtcc cacttcttgt ttcggatctc gatgcgcgcc tgcccgtaga
     1981 tcgcgggcca gtagccggag ttctgcttgt aggccttgtt gatgtgcgtg acgccctcgt
     2041 cccagcggtc ggcgtcgatc aacaggatgc ccagtccggc gtccgcttcg gggaatccct
     2101 ttccactctc gcgcccctga atgtcgagcg cctcgcggta aagattctcc gcctcttcga
     2161 agcgccccga gcgcgtggcc acttcggcca gtccgacctt cgctttgggc agctcgtagc
     2221 tgttggtgac ggcttcgtcg aaagccgcct tggccgccgc gaggtcgttc ttctcgagcg
     2281 ccttggtgcc gactcggacc ggatcggtct cgtacgtgta gctcttctgc gcccaactca
     2341 ccgaggtcgc cagacaaggg acgacgaacg cggcgatggc aacgtgacga agcagggaca
     2401 tcggacctcc ttgggtctct actagttggc cttgctgccc aacggttttc gcgtgagata
     2461 aatctgacgg atcgactgtc gcgccgggag aaatccggcc cgttcgacgt ggcgctggcc
     2521 acggtcgctg gtcagatgcg tcacgaacat cgagccgcgt atgctgccgc cggcaaggca
     2581 tgcgacgtac agataatgat acaacggata ctctccgtag ccgatctgct cgtagcccgg
     2641 ctcgaccgga accaggccgg tgtccgggag gacgggtacc gcgcgcacgg accgcccccc
     2701 gcccatgtcg gggaggctgt cgggcatgct caaggtgctc gccagaccga gcccgcgcgg
     2761 atcggcgagg accgcctcga tcaccgcggc ctcgtccgcg agaaacgtga tgtgcgccag
     2821 ctcgggcgcc gcgttgcccc cggggcgggc gacgtcttcg gtcaccgcga ggctcgcgcg
     2881 gaacgcgtcc cacaacccct ggttggggtc gggcgcgtag agccggtcga agcgcggatc
     2941 ggcctggtcg cgcgcgatcg cgcgcagctc ttccatggcg aacgattcga ccccggacag
     3001 cgagttggcc aacacggcca cgccgccgag ggcgatcggg aagcacagca ccgtgtcgtc
     3061 gacggcaatg cgcaccagcc gccgttcgtc aggagtcggc agccgataga gaaatcccac
     3121 ctgggcgcgg ccattggcca agtgctcgag cgcgtgcacg gttccaccgt cctcgaggcg
     3181 cacgtgcagc ttcgggtaga tatcgtggta tttctttatc agttgcggca tgagatc
//
LOCUS       DASPGK010000141         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1441712, whole genome shotgun sequence.
ACCESSION   DASPGK010000141 DASPGK010000000
VERSION     DASPGK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441712"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1181)
                     /gene="hrcA"
                     /locus_tag="VEC56_04075"
     CDS             complement(159..1181)
                     /gene="hrcA"
                     /locus_tag="VEC56_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXV13364.1"
                     /translation="MLNEKDKPILRTIIDLYVGDAVPVSSQRVHDSGRFRMSTATIRN
                     RMVVLEREGFIAKAHVSSGRIPTDEGYRAYVDELQAGTVRPRGDGLAAVREELRDDLD
                     VNGVMQRASQLLGALSQHVAVVYGAIVQESRVRTVRLLRLEGAKIIVVVHLIPDYERT
                     VTLRLGRDVAAEAVAAAERLLDRMVAGRSLQEARARLAEMVRDNVTDEGIVVGEIALR
                     RDDVFSEPVAVELCFEEPAQMLAQPELSDPRTLQLLLRILHDRGYLTSILADRRLNSV
                     EVTIGSEHRDDTLRPFSLVTAGYRMGAARGVLGLIGPTRMRYDLALGLVESMSRELCA
                     IGDERF"
     gene            complement(1175..>2157)
                     /locus_tag="VEC56_04080"
     CDS             complement(1175..>2157)
                     /locus_tag="VEC56_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HXV13365.1"
                     /translation="LVDEISVAFAEIGPASLRTVFVGGGTPTVLEPHEWERVIAAIAP
                     HVPRDGVAEFTVEMNPEDVDARVVGFLRGLGVNRASIGVQSMDAVGQKVLKRCAPEVN
                     ERAIDLVRERFDNLSFDVLLGVPKTTIDDLRDTLRRLLPKEPTHLSVYGLEPGGDLGE
                     EVERFFRAVDPDRVADEYLEVCSLLAAAGYRHYEVSNFCRPGFESLHNRVYWDGGDYL
                     GVGPAAHSSIGGERFANAPSIDSYLARRGAEHLAARLRDPGGDQRLESAMLALRTDRG
                     IARDACEPRAVADIIDDGLAVVERGRIRLTDRGYLMLNDVVLRLVSTTPC"
BASE COUNT          343 a          758 c          704 g          352 t
ORIGIN      
        1 tcgggatcgg cgacgaccgg ctccgcggag tccgtatcca gatcgtacac gcgatcgttc
       61 ggcgcctccg tggcgtcggt gacctcgcgg tcgatgtcgg tgggctgcgg tgtgtctttg
      121 tctttcacgc gttccgctct cctgctcgat gatgatgtct agaagcgctc gtccccgatc
      181 gcgcacagct cgcgcgacat cgattcgacc aaccccaggg ccaggtcata gcgcatgcgg
      241 gtcggcccaa tgagcccaag taccccgcgc gcggcgccca tgcgataccc cgccgtcacc
      301 aagctgaagg gacgcagcgt gtcgtcccgg tgctcgctgc cgatggtgac ctcgacgctg
      361 ttcagccgcc ggtcggccag gatcgaggtc aaatagccgc ggtcgtgcaa gattcggagc
      421 aaaagttgga gcgtccgggg gtcggacagc tcgggttggg cgagcatctg agccggctcc
      481 tcgaagcaaa gctcgaccgc gaccggttcc gagaagacgt cgtcacgccg cagcgcgatc
      541 tcgcccacca cgatgccctc gtcggtcacg ttgtcccgga ccatttcggc cagccgggcg
      601 cgcgcttcct gcaggcttcg tcccgcgacc atgcggtcga ggaggcgctc cgcggccgcc
      661 accgcttcgg ccgcgacgtc gcgacccagg cgcagcgtga cggtgcgctc gtaatcgggt
      721 atcaggtgca cgacgacgat gatcttggcg ccttccaggc gcaggagacg caccgtgcgc
      781 acgcggctct cttgcacgat ggcgccgtag accaccgcga cgtgctgcga gagcgcgccc
      841 aggagctgcg acgcgcgctg catgacgccg ttcacgtcga ggtcgtcgcg cagctcctcg
      901 cgcacggccg cgagcccgtc accgcgcggg cgcaccgtgc ccgcttgcag ctcgtcgacg
      961 taggcgcggt agccttcgtc ggtggggatg cggcccgacg acacgtgggc cttggcgatg
     1021 aacccctcgc gctcgagcac caccatgcgg ttgcgaatgg tggccgtact catgcgaaaa
     1081 cgccccgagt cgtgcacgcg ctgcgagctg acgggcaccg cgtcgccgac gtagaggtcg
     1141 atgatggtgc ggagtatggg cttgtccttt tcgttcagca tggcgttgtc gaaaccaatc
     1201 gcaacacgac gtcgttgagc atcaagtaac cacggtcggt gagccggatt cgtcccctct
     1261 cgactaccgc gagaccgtca tcgataatgt ccgccacggc gcgcggttcg cacgcgtcgc
     1321 gcgcgatccc gcggtcggtg cgcagcgcca gcatcgcgga ctcgagccgc tggtcgccgc
     1381 ccgggtcgcg aagccgcgcg gccaagtgtt ccgccccgcg ccgtgcgagg tacgaatcga
     1441 tcgacggcgc gttcgcgaaa cgctcgcccc cgatcgacga atgggccgcg ggaccaaccc
     1501 cgaggtaatc gccgccgtcc cagtacaccc gattgtggag gctctcgaac ccgggccggc
     1561 agaagttcga cacctcgtag tgtcgatatc ccgccgcggc caggagcgag cacacctcca
     1621 agtattcatc agccacgcgg tccgggtcaa ccgcgcgaaa gaagcgctcg acctcttcgc
     1681 cgaggtcgcc gcccggctcc agcccgtaca ccgacaggtg cgtgggttct ttgggcagga
     1741 gccggcgcag ggtgtctcgc aggtcgtcga tcgtggtctt cggcaccccc aataggacgt
     1801 cgaagctgag gttgtcgaag cgctcgcgaa ccaggtcgat ggcgcgctcg ttgacctcgg
     1861 gcgcgcaccg cttcagcacc ttctgcccca ccgcgtccat cgattgcacg ccgatgctcg
     1921 cgcggttcac tcccaggccg cgcagaaaac cgacgacgcg cgcgtcgacg tcttccgggt
     1981 tcatctcgac tgtgaactcc gcgacgccgt cgcgcggaac gtgcggcgcg atggcggcga
     2041 tcacgcgttc ccactcgtgc ggttccaaca ccgtcggcgt cccacccccg acgaagacgg
     2101 tccgcagcga cgccggcccg atctcggcga atgcgaccga tatctcatcg acgagcg
//
LOCUS       DASPGK010000142         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_974036, whole genome shotgun sequence.
ACCESSION   DASPGK010000142 DASPGK010000000
VERSION     DASPGK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974036"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VEC56_04085"
     CDS             complement(<1..553)
                     /locus_tag="VEC56_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV13366.1"
                     /translation="MLNLEITWEGEAHRVALDNGEHAVGRSSENTVQVAMARVSKKHA
                     LLRVDGDRLFVKDLGSRNGTEINGNPVGDQWTEVPAGSLVSFAGALMRRATPASTAAH
                     QLLGDHQVSPSLRYNMSQGFSRDAQSRLMDRSSQLFELLASSDDAMAIETAACRFVAE
                     SVPAERVVMLSDAGEATQVEARAR"
     gene            complement(723..2345)
                     /locus_tag="VEC56_04090"
     CDS             complement(723..2345)
                     /locus_tag="VEC56_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HXV13367.1"
                     /translation="MATNTKQGSTHVVDKQEWGTRVGIILAVAGSAVGLGNFLRFPGQ
                     AVQNGGGAFMIPYFCALLFLGIPIGWAEWTMGKYGGQKGFRSAPSILGVIGGGAAARY
                     LGVIGVLIPLAVSFYYTYIEAWCLGYFWHYLTGGIGIDASAPITDQTARASQFYNDLT
                     GTAHNGVIAGGSIKTFIFWTITFAINIWLVYRGVSKGIERFVSWAMPLMAVCALIVLA
                     RVLTLGTPDPAYPDQNVVSGLAYMWNPSFDALLNPQTWLAAAGQIFFSLSVGFGVIIN
                     YAAYLRKNDDVVLSGLTASSTNELFEVGFGGLITITAAFIFLGTAGMIGAVQGGSFGL
                     GFTTLPVVFVHMGSFGNFIGAVWFLMLFIAAITSSISMYQPSLAFFQESLGWTRRGSN
                     ALMVTVCVIGSFLVMYFSERGLFWSTIDDWVGTFLIFILAMVQIICFSWIFGVDRGLK
                     YAHKGAHIRIPGFYRFIMKYVTPTYLLVVFGAFCWMNLGSWIRAVAAEPLKQGAMVLI
                     LAVIVLLVVCVRVGEKRWRAAGMDVDGKIIEE"
     gene            complement(2471..3325)
                     /locus_tag="VEC56_04095"
     CDS             complement(2471..3325)
                     /locus_tag="VEC56_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HXV13368.1"
                     /translation="MTTKGADLFGFLKHKRRARLRDQPVPSQWPRILEANVPVYRRVP
                     QALRTQLHGHMHVFLAEKHFEGCDGLEMTDEIRVTVAAQACLLILDREANYYPDLVTI
                     LVYPSVFYADVEEPDGAIVSEYKEDRSGESWDLGVVILSWEDVVEYASTGRAGYNVVL
                     HEFAHQLDMENGAMDGMPKLQGPAQRTEWARVFSQAYESFEQAASSNRGTLIDSYGAG
                     DPAEFFAVVTESFFERPLALKREYAALYDTLAHYYRVDPASWEPPGSVAAEWAAPRRA
                     RGRRRRRG"
     gene            3370..>4217
                     /locus_tag="VEC56_04100"
     CDS             3370..>4217
                     /locus_tag="VEC56_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HXV13369.1"
                     /translation="MPASNHPRILLGLLLGAVAGVVTNVVTDGGEATQRFVSVVTEPA
                     GRMWLSALIMTVIPLILSSLSLGVAGLGDLRQVGRIGLVTLSIFVGLTAVAAVIGLTL
                     TNTFRPGEGLDPAMRTELMDTYGGEAKNAAGLSGSGLSMDMLVKIVPRNPVKAAAEGD
                     MLAVIFFSLMLGIGIASVPREKSGALASFLESLGYVTVEIIHLVMRLAPIGVFCLIYS
                     VTARFGFDLLLQLFKYVGTVLAGLTIMMTVTFGIVLKLGAKRNPLDFMKKARVVIVTA
                     FSTSSS"
BASE COUNT          773 a         1368 c         1341 g          735 t
ORIGIN      
        1 agcgcgcgcg cgcttccact tgggtcgcct caccggcgtc agacagcatg acgacgcgct
       61 cggcgggaac gctctccgcg acgaaacgac acgcggcggt ctcgatggcc atggcgtcgt
      121 cgctggaggc gagcagctcg aagagctgcg agctgcgatc catgaggcgg ctttgcgcgt
      181 cgcgcgaaaa gccctggctc atgttgtagc gtaggctggg agagacctgg tgatcaccca
      241 ggagctggtg cgcggccgtc gaggcgggcg tagcgcgccg catcagcgcg cccgcgaagc
      301 tcaccagcga gccggcaggc acttcggtcc actggtcgcc caccggattg ccgttgatct
      361 cggttccgtt gcggctgccg agatctttga cgaacaggcg atcgccgtcg acgcgaagca
      421 gcgcgtgctt cttgctcacg cgcgccatcg cgacttggac ggtgttctcg ctcgagcgcc
      481 ccaccgcgtg ctcgccgttg tccaacgcga cgcgatgggc ttctccttcc caggtgattt
      541 cgaggttcag catagctgtg tgacgccggc ggccggcggc tggtcggttc gaattagggc
      601 tgcgcgtcct cgcgtttgcc gctggtcgtc atgaccttgt agaaacacca ccccgtcagc
      661 ccccacacga aagcgagaga cccgagcaag aatagccatc ccatcgtcgt cattggcgtc
      721 tcctattctt cgatgatctt gccgtcgaca tccatgccgg ccgcgcgcca gcgcttctcg
      781 cccacgcgga cgcacaccac cagcagcacg atgaccgcca ggatcaggac catcgcgccc
      841 tgcttgagcg gttcggccgc gacggcgcgg atccaagacc ccaggttcat ccagcagaac
      901 gcgccgaaga cgaccagcag ataggtcggc gtcacgtact tcatgatgaa ccgatagaaa
      961 cccggaatcc ggatgtgtgc gcccttgtgc gcgtacttga gtccgcggtc gacgccgaag
     1021 atccagctga agcagatgat ctgcaccatg gcgaggatga agatcaagaa agtcccgacc
     1081 caatcgtcga tggtgctcca gaacagtcca cgctcgctga agtacatgac gaggaaggaa
     1141 ccgatcacgc acaccgtcac catgagcgca ttggaaccac ggcgcgtcca gcccagcgac
     1201 tcttggaaga acgcgagcga cggctggtac atcgagatcg agctggtaat cgccgcgatg
     1261 aacagcatca ggaaccagac cgcgccgatg aagttcccga aggagcccat gtggacgaac
     1321 acgaccggaa gggtcgtgaa gccgaggccg aacgaaccgc cctgcacggc gccgatcatg
     1381 ccggccgtgc cgaggaagat gaacgcggcg gtgatcgtga tcagaccgcc gaagcccact
     1441 tcgaacagct cgttggtcga cgacgcggtc aaacccgaga ggacgacgtc gtcgttctta
     1501 cgcaggtagg ccgcgtagtt gatgatcacg ccgaaaccga ccgaaaggct gaagaagatt
     1561 tgcccggccg cggccagcca cgtctgcggg ttcaagagcg cgtcgaagct cgggttccac
     1621 atgtaggcga gcccactgac cacgttctga tcggggtacg cggggtcggg cgtaccgagc
     1681 gtcagcacgc gcgcgagcac gatgagtgcg cacaccgcca tcagcggcat ggcccaactc
     1741 acgaagcgct ctatcccctt cgatacgccg cggtatacca gccagatgtt gatcgcgaag
     1801 gtgatggtcc agaagatgaa ggtcttgatc gagcccccgg cgatgacgcc gttgtgcgcg
     1861 gtgccggtca ggtcgttgta gaactgtgac gcgcgcgcgg tctggtcggt aatgggcgcg
     1921 ctcgcgtcga tgcctatgcc ccccgtcagg tagtgccaga agtagcccag gcaccacgcc
     1981 tcgatgtacg tgtagtagaa cgacaccgcg agcggaatca gcacgccgat gacacccaag
     2041 taacgcgcgg cggcaccgcc gccgatcaca cccaagatcg agggggcgga gcgaaatccc
     2101 ttctgaccgc cgtacttccc catcgtccac tccgcccagc cgatcggaat gccgaggaag
     2161 agcagcgcgc aaaaatacgg aatcatgaac gcgccgccgc cgttctgcac ggcttggccg
     2221 gggaaacgca ggaaattccc gagtcccacg gccgagccgg caacggcgag gatgatgccg
     2281 acgcgcgttc cccactcctg cttgtcgact acgtgggtcg agccttgctt cgtgttcgtg
     2341 gccattggtc cctacttgga aggagtaagc gccgacgcgc acgggtccca gctccctgcg
     2401 cggcacgagg ttcgggtgcc gatggtatcg gcgcgccggg ggtgggtcaa cgaccacttt
     2461 cggccctggg ctaaccgcgg cggcggcggc ggccgcgcgc gcgccgaggt gccgcccact
     2521 cggccgcgac cgagcccggc ggctcccacg aggccggatc gacgcgatag tagtgcgcga
     2581 gcgtgtcgta cagcgcggcg tactcgcgct tgagagcgag cggccgctca aagaaactct
     2641 ccgtgacgac ggcgaagaac tcggccggat cgccggcgcc gtagctgtcg atcagcgtac
     2701 cgcgattgga cgacgccgcc tgctcgaagg attcataggc ttggctgaac acgcgcgccc
     2761 attcggtgcg ctgcgccggg ccctggagct tcggcatgcc gtccatggcg ccgttttcca
     2821 tgtcgagttg gtgcgcgaac tcgtgcagaa cgacgttgta gcccgcacgg cccgtcgacg
     2881 cgtactcgac cacatcctcc caagacagaa tcacgacccc caggtcccac gattcgcccg
     2941 atcggtcttc tttgtattcg ctcacgatgg ctccgtcggg ttcctcgacg tcggcgtaga
     3001 acaccgacgg atagaccagg atggtgacga gatcggggta gtaattcgcc tcgcgatcga
     3061 gaatgagcag gcatgcttgg gccgcaaccg tcacgcggat ctcgtccgtc atctccaggc
     3121 cgtcgcaccc ttcgaagtgc ttctcggcca gaaagacgtg catgtgtccg tgcagctgcg
     3181 tgcgcagcgc ttggggaacg cgacggtaca caggaacatt ggcctcgaga atgcgcggcc
     3241 actgggatgg aacgggctgg tcgcgcagac gcgcgcgccg cttatgcttc agaaaaccga
     3301 acaggtccgc tcctttcgtg gtcacccgag tttgacacgt tcggaccgag ggtgctagcg
     3361 tcgagggcca tgccggcctc caaccatccc cgcattctgc ttgggcttct gctcggcgcc
     3421 gtcgcgggtg tcgtcaccaa cgtcgttacc gacggcggtg aagcgacgca gcgcttcgtc
     3481 tccgtggtca ccgaacccgc ggggcgtatg tggctttccg ctctcatcat gacggtgatt
     3541 ccgctcattc ttagctcgct gtcgctgggt gtcgcggggc tgggcgatct tcggcaagtc
     3601 ggtcgcatcg gcctggtgac cttgagcatc ttcgtcgggt tgacggcggt cgccgcggtg
     3661 atcgggttga cgctgaccaa caccttccga cccggcgagg ggctcgatcc cgccatgcgc
     3721 accgagctca tggacaccta cggcggcgaa gccaagaacg ccgccgggct ctcgggcagc
     3781 ggcctctcca tggacatgct ggtcaagatc gtgccgcgca atccggtcaa ggccgcggcc
     3841 gagggagaca tgctggcggt catcttcttc tcgctcatgc tcggcatcgg catcgcgtcg
     3901 gtgccgcgcg agaagtcggg cgctctggcg tcgttcctgg agagcctggg ctacgtcacc
     3961 gtcgagatca tccacctcgt catgcggctg gcaccgatcg gcgtcttctg tctcatctac
     4021 agcgtcaccg ctcgtttcgg cttcgactta ctcttgcagc ttttcaagta cgtcggcacc
     4081 gtgctcgcgg gactcacgat catgatgacc gtcacgttcg gcatcgtgct gaagctcggc
     4141 gcgaaacgca acccgctcga cttcatgaag aaagcacgcg tggtcatcgt cacggctttt
     4201 tcgacgtcgt ccagcaa
//
LOCUS       DASPGK010000143         6916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_39046, whole genome shotgun sequence.
ACCESSION   DASPGK010000143 DASPGK010000000
VERSION     DASPGK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39046"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VEC56_04105"
     CDS             complement(<1..500)
                     /locus_tag="VEC56_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13370.1"
                     /translation="MLRAILAAGLSVAILHAPAWTRDTGSEPIRTSLPVAEAGATDGS
                     LTPDPRFALAAANTTLLASYSFDVGATCNRQGWTVVDATAQIAEFWHVDDFAGANVNP
                     GDSLAVLAGSKSLWCGVRASTSGLTCGYALLPGYGNGWDQLWVTKACLSVTGTLDVSF
                     LMETDS"
     gene            629..1498
                     /locus_tag="VEC56_04110"
     CDS             629..1498
                     /locus_tag="VEC56_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13371.1"
                     /translation="MLQAIPYHLGAFFARFTPDWFCRAITWTLAEINWRLRPGTRRTV
                     AENLRVIHPEWTESERRRAARQVVHNFAKSILLFLQLPFFDPDELFARLDMSALRAEL
                     EKLGPKRSFIVASAHVGPWELGGFALTRMGYTAHTVALDHPSAQVAKFYSDRRAYVGV
                     RAHPLSGSFMVLKEAIDRGEVVALMIDRAYGKAKKRFAMFGLPTEVPLGHLVLSARCR
                     APVLTGVLVFDGPKRFRYVHGGTHFPEDHEDEFEKLERLQEECLRDLERLIRAHSEQW
                     FHFMPIVRTEAST"
     gene            1502..2194
                     /locus_tag="VEC56_04115"
     CDS             1502..2194
                     /locus_tag="VEC56_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV13372.1"
                     /translation="MNTRALPYDDLAAVIPAYHAARHLAPVIDHVARYVPRNRIVVVD
                     DGSKDDTRAVAERAGVVVEVHPENRGKGAAIRSGIARAQTLGVPLAILMDADGQHSPD
                     EIPAFVDCQKRTGADVVVGNRLEDPANMPWLRKATNYVTSAVVSLLAGRRVPDSQNGY
                     RLIRLDHFKKFPLTTSRYEIESEMIIRAGRAGGKFASVPIRTIYAGEASFINPFVDTM
                     RFLRMVGKSFFW"
     gene            2220..2969
                     /gene="surE"
                     /locus_tag="VEC56_04120"
     CDS             2220..2969
                     /gene="surE"
                     /locus_tag="VEC56_04120"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXV13373.1"
                     /translation="MAKHILICNDDGFDARGIQVLERALSPLGEITVVAPDAEQSASS
                     HSLTVRRPIDVKQVDARHFRVVGTPTDAIVLALQVLLERKPDLIVSGINHGPNMGEDV
                     QYSGTVAAASEGMILGVPSIAVSALQRTVANEEVNGRFARIIVERALAEGLPKDVLLN
                     VNIPNPDVAPIKGLRITKLGSRHYENFIDPGRGPLQKSYTIGGREPVWRDDDGSDISA
                     VRLGFVSVTPLHLDPTHYKAIVEMERWRFEL"
     gene            2983..3672
                     /locus_tag="VEC56_04125"
     CDS             2983..3672
                     /locus_tag="VEC56_04125"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXV13374.1"
                     /translation="MRWRQYKIARERMVREHVYNRGVRDPRVLEAMMRVPRHLFIGRD
                     AGIEAYADHSYPIGFQQTMSQPYMVAYLTEELRLSGEETVLEIGTGSGYHTAVLAALS
                     KNVATVERVPELAERAQSVLRDLLFSNVDVKTDDGWKGWPEKGPFDRVLLTAAAQQVP
                     RALLAQLSDAGFLLGPVIRADGKQEIVRLIRRGGKVEIERLIECSFVPLIRTVGRTGR
                     SKRTETGVSRS"
     gene            3799..3876
                     /locus_tag="VEC56_04130"
     tRNA            3799..3876
                     /locus_tag="VEC56_04130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3834..3836,aa:Pro,seq:cgg)
     gene            3920..4804
                     /locus_tag="VEC56_04135"
     CDS             3920..4804
                     /locus_tag="VEC56_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV13375.1"
                     /translation="MSNDRPHNDERAEPSYDIQARYLAEIGRYELLTPEQEVELGRAL
                     AEGSEDAKRRLIIANLRLVVTIAKRYQRGSQNLLDLIEDGNLGLIRAAAKFDYTKGFR
                     FSTYASYWIKQSIKRGIASQSRAIRIPVHIYHLINRYLKVEDRHAGKSLDDEAMSAEL
                     DVSVRRVKLVRTLIHGIRAEDPLASADALQRLASDAYHRGNGTPESLVTLQLENEEMS
                     TLFDRCLNPREQEVLTLRYGLDDGEPRTLGETGREVGVSRERVRQIEKRALQKLNLVL
                     SGRKRAGGSAREPREGSN"
     gene            4801..5334
                     /locus_tag="VEC56_04140"
     CDS             4801..5334
                     /locus_tag="VEC56_04140"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXV13376.1"
                     /translation="MNQAAVERIRASIRDVPDFPKPGIVFKDITPILGDPTLFGEVIR
                     VFVDRYRTMRIQKVAAMESRGFIFGAPLAATIGAGFVPLRKHGKLPHTTLAESFTLEY
                     GEETLEIHTDAIRKGERVLVIDDLLATGGTAAAAASLVGKTGGTVVEFAFLIELAFLN
                     GRERLAGFPAYAMIRFD"
     gene            complement(5350..5426)
                     /locus_tag="VEC56_04145"
     tRNA            complement(5350..5426)
                     /locus_tag="VEC56_04145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5390..5392),aa:Arg,seq:cct)
     gene            5788..>6916
                     /locus_tag="VEC56_04150"
     CDS             5788..>6916
                     /locus_tag="VEC56_04150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002782720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HXV13377.1"
                     /translation="MSNRYLRLGAFAVLFVAIASWALTAGGQQPQSYVPRQVIIKLEA
                     STPTADALAVRSSLRASVKQRFKSSGAELWVIDGIEVNEAIARFKDDPRVDYIEPNYT
                     VRALDVFPNDPRFDDLWGMHNTGQTGGTPDADIDAPEAWELGTGGGVLVGVIDTGVDY
                     NHVDLAANMFINPNEIPGNGVDDDFNGYIDDVRGWDFVNNDNNPMDDHGHGTHVSGTI
                     GALGNNGVGVVGVSWSVRIMGLKFLDAGGFGSTSDAILAVEYATMMGVKLTSNSWGGG
                     GFSQALQDAIEAAGNAGILFVAAAGNASSNNDVFPNFPSNYPLDNIIAVASTDHRDNL
                     SGFSNFGLTTVDLGAPGTDILSTFPNNTYGTISGTSMATPHV"
BASE COUNT         1252 a         2242 c         2263 g         1159 t
ORIGIN      
        1 tccgaatcgg tctccatgag gaacgacacg tcgagcgtgc cggtgaccga aaggcacgcc
       61 ttcgtcaccc acagttgatc ccagccgttc ccgtagcccg gcagtaacgc gtatccgcac
      121 gtcagtccgc tcgtcgatgc acgcacgccg caccagagcg acttcgatcc cgcgaggacc
      181 gcgagcgagt cgcccggatt cacattggcg cccgcgaagt cgtcgacgtg ccagaactcc
      241 gcgatctgcg cggtggcgtc gaccacggtc cagccctgac ggttgcaggt ggcaccgacg
      301 tcgaaggagt agctggcgag gagggtggtg ttcgcggccg cgagcgcgaa gcgcggatcg
      361 ggagtgagcg aaccgtcggt cgcgcccgcc tcggcgacgg gcaacgaggt gcgtatgggt
      421 tcggagcccg tgtcccgggt ccaagcgggg gcgtgcagaa tcgccaccga gagtcctgcg
      481 gcgaggattg cccttagcat gattcgaagc ctccagtcgg tcggccggtc cggggcgctg
      541 acagagggaa gtgcgccgct tcgggccaaa acggctaacc cgaaccgcct tgcggggcta
      601 gcggggccgg ggtatggtcg gcggacccat gctgcaggcc atcccgtacc acctcggcgc
      661 cttcttcgcg cgcttcaccc ccgactggtt ctgccgggcc atcacgtgga ccttggccga
      721 gatcaactgg cgcctccgcc ccggaacccg ccggacggtg gccgagaacc tccgcgtcat
      781 ccatcccgag tggaccgaga gcgagcgccg gcgcgccgcg cggcaggtgg tccacaattt
      841 cgccaagagc attctcttgt tcctccaact cccgttcttc gaccccgacg agctgttcgc
      901 gcgcctggac atgtcggcgc tgcgcgccga gctggagaag ctggggccga agcgatcgtt
      961 catcgtcgcc tcggcgcacg tggggccgtg ggagctgggg ggcttcgcct tgacgcgcat
     1021 ggggtacacg gcgcacacgg tggccctcga ccacccgagc gcgcaggtgg caaagtttta
     1081 cagtgaccgt cgcgcctacg tgggcgtgcg cgcgcatcca ctgtcgggga gcttcatggt
     1141 gctcaaagag gcgatcgacc gcggcgaggt ggttgcgttg atgatcgatc gtgcctatgg
     1201 caaggctaag aaacggttcg cgatgttcgg cctcccgacc gaggttccgc tggggcattt
     1261 ggtcctttcg gcgcgctgtc gcgcgcccgt gttgaccggc gtactcgtgt ttgacggccc
     1321 gaagcgcttc cggtacgttc acggcggcac gcactttccc gaggaccacg aggacgaatt
     1381 cgagaagctg gagcgcttgc aggaggaatg cctgcgcgac ttggagcgct tgattcgcgc
     1441 gcacagcgag cagtggttcc acttcatgcc catcgttcga accgaagcga gcacgtagtc
     1501 gatgaacacg agagccttgc cctacgacga cctcgccgcc gtcatccccg cctaccacgc
     1561 ggcccgtcac ctcgcgccgg tgatcgacca cgtcgcgcgc tacgtgccgc gcaaccgcat
     1621 cgtggtggtc gacgacggct cgaaggatga cacgcgcgcg gtggcggaga gggcgggtgt
     1681 ggtggtcgaa gtccacccgg agaatcgcgg caagggggcc gcgatccgct cggggatcgc
     1741 gcgcgcgcaa accctcgggg tgccgctcgc tattttgatg gatgcggacg ggcagcacag
     1801 cccggatgaa atcccggcct tcgtcgactg ccaaaaaaga accggcgccg acgtggtggt
     1861 ggggaaccgc ctcgaggacc cggccaacat gccgtggctt cgcaaggcga ccaactacgt
     1921 gaccagtgcg gtggtgtcgc tgttggcggg ccgccgcgtc ccggactctc aaaacggcta
     1981 ccggctgatc cggctcgacc acttcaagaa gttcccgctg accacctcgc gctacgaaat
     2041 cgagtcggaa atgatcatcc gggccggccg cgcgggcgga aaattcgcct cggtgccgat
     2101 ccggaccatc tacgcgggcg aggccagctt tatcaaccca tttgtcgata cgatgcggtt
     2161 tttgcgcatg gtcgggaagt cgttcttctg gtagaatggc acctccttcc ggcggttcga
     2221 tggccaagca catcctgatt tgcaacgacg acggcttcga cgcgcgcggc attcaagtgc
     2281 tcgagcgagc gctctcgccg ctgggggaga tcacggtcgt ggcaccggat gccgagcaga
     2341 gtgccagcag ccactcgctc accgtgcggc gcccgatcga cgtcaagcag gtggacgcgc
     2401 gccactttcg cgtcgtgggc acgccgaccg acgcaatcgt gctcgcgttg caggtgctgc
     2461 tcgagcgaaa accggatctc atcgtgtcgg ggatcaacca cggacccaac atgggggagg
     2521 acgtgcagta cagcgggacg gtggcggcgg cctcggaggg gatgattctc ggggtcccgt
     2581 cgatcgcggt ctccgcgctc cagcgcacgg tcgcgaacga agaggtcaac gggcgcttcg
     2641 cgcgcattat agtggagcgc gcgctggcag aagggcttcc caaggacgtc ctgctcaacg
     2701 tcaacatccc gaatccggac gtcgccccca tcaaggggct gcgcatcacc aaactgggct
     2761 cgcgccacta cgagaacttc attgatcccg ggcgcggccc cttgcagaag agctatacca
     2821 tcggcgggcg ggagccggtg tggcgcgacg acgacggttc ggacatctcg gcggtgcggc
     2881 tgggcttcgt gtcggtgaca ccgctgcacc tggacccgac gcactacaaa gcgatcgtcg
     2941 aaatggaacg ctggcggttc gaactgtgag aggatcctcg cgatgagatg gcggcagtac
     3001 aagatcgcgc gcgagcggat ggtgcgcgag cacgtctaca accgcggcgt gcgcgacccg
     3061 cgcgtcttgg aagcgatgat gcgcgtgccg cgacatttgt tcataggacg cgacgcgggc
     3121 atcgaagcgt atgcggatca ttcgtaccca atcgggttcc agcagacgat gtcgcagccg
     3181 tacatggtcg cctacctgac cgaagagctg cggctttcgg gcgaagagac cgtgctcgaa
     3241 atcggaaccg gctccgggta tcacaccgcg gtgctggccg cgctgtcgaa gaacgtcgcc
     3301 acggtggagc gcgtacccga attggccgag cgcgcgcagt ccgtgctgcg cgacctcttg
     3361 ttctccaacg tcgacgtcaa gaccgacgac gggtggaagg gctggcccga gaagggtccc
     3421 ttcgaccgcg tgctcttgac cgcggccgcg cagcaggtgc cgcgcgcgct gctcgcgcaa
     3481 ctctccgacg cgggctttct cctggggccg gtgattcgcg cggacggcaa acaggagatc
     3541 gtccgcctga ttcgacgtgg aggcaaggtt gagattgaac gcctgattga atgctcattc
     3601 gttcctctga ttcggaccgt cgggcgaacc gggcgttcga agcgcacgga aactggcgtg
     3661 tcgcggtcct gacggaccgc cgaatctagg gttgattcgt cgagaaaacg tgttacgata
     3721 aacgaactta gagcctcgcc cgccggcctc gtggaatcgg acggagggca gaggcattat
     3781 attgtgcggg cttggggtcg gggcgtagcg cagtccggtt agcgcgcctg cttcgggagc
     3841 aggaggtcgg aggttcgaat cctctcgccc cgaccattca cccctggaac gccggcgggg
     3901 ttcgacgaac acgacgacga tgagcaacga tcgccctcac aacgacgaac gcgcggagcc
     3961 gtcttacgac atccaggcgc gttacctggc cgagatcggc cgctacgagc tgttgacccc
     4021 cgagcaggag gtcgagctgg ggcgagcgct ggccgaaggc agcgaggatg ccaagcgccg
     4081 tctcatcatc gccaacctgc gactcgtggt caccatcgcc aagcgctacc agcgcggcag
     4141 ccaaaacctc ctcgatctaa tcgaagacgg caacctgggg ctgattcgcg cggccgcgaa
     4201 attcgactac accaagggat tccgcttcag cacctacgcg tcctactgga tcaagcagtc
     4261 gatcaagcgc gggatcgcga gtcagtcgcg cgcgatccgc attcccgttc acatttatca
     4321 cctcatcaat cgctatctca aggtggagga ccggcacgcg gggaagtcgc tcgacgacga
     4381 agcgatgtcg gccgagctgg atgtcagcgt gcggcgcgtg aagctggtgc gcacgctgat
     4441 ccacggcatc cgcgccgaag atccgctggc gagcgcggac gcactccagc gcctagcctc
     4501 cgacgcctac caccgcggga acggcacgcc cgagtcgctg gtgacgcttc aattggagaa
     4561 cgaggagatg tcgacgctct tcgatcgctg cttgaatccg cgcgagcagg aagtcttgac
     4621 gcttcgctac gggctcgatg acggcgagcc gcgcaccctg ggcgagaccg gccgcgaagt
     4681 gggcgtctcc cgcgagcgcg tgcgtcagat tgaaaagcgc gcgctgcaga agctcaacct
     4741 cgtcttgtcg gggcgcaagc gggcgggagg ctcggcccgc gaacctcgcg agggttcgaa
     4801 ttgaatcagg ccgcggtcga gcgcatccgt gcctccattc gtgacgtccc cgattttccc
     4861 aagcccggca tcgtcttcaa ggacatcacg ccgatcctcg gcgacccgac cctcttcggc
     4921 gaggtgatcc gcgtcttcgt cgaccgctac cgcacgatgc gcatccagaa agtggcggcg
     4981 atggagtcgc gcggcttcat cttcggcgcg ccgctggcgg cgacgatcgg cgcgggcttc
     5041 gtgccgctgc gcaagcacgg caagctcccg cacaccaccc tggccgaatc gttcacgctc
     5101 gaatacgggg aagaaacgct cgagatccac accgacgcca ttcgcaaggg cgagcgcgtg
     5161 ctcgtgatcg acgacttgct cgcgaccggc ggcaccgcgg ccgccgcggc cagcctggtc
     5221 ggaaagacgg gcggcaccgt ggtcgaattc gcgttcctga tcgagctcgc gttcctcaac
     5281 ggacgcgaac gcttggccgg gtttcccgcc tacgccatga tccgcttcga ctgacgcact
     5341 attaaggagt ggtggccccg gcgcgagtcg aacgcgcgac aaacggttta ggaaaccgct
     5401 gctctatcca tctgagctac ggggccactc gaggagactt aacaaaactc acttcatgtg
     5461 aatcggtgcg ccagtatacg aaaatcgtgt agtcgtcgct agacattcat aaaccacatc
     5521 tgtatactcg gccctcggag taccaggcct cgcttttcac tggcccacca aggaggtcta
     5581 tgtgaaggca gtacggctcg cccccggtac ctccgtctgt tcctttccaa tgttcggtcc
     5641 gccgcattcg cggctggacc gacaggctcg cgcccgaacc aaccccgcga ccatactccc
     5701 tctgcgttcg cgcgagtcgc acgcccctac acgccaattg cgcacaccgt gcgcagttcc
     5761 attgtcacca caatgagagg gatcgtcatg tcgaatcgct atctccgtct cggtgcattc
     5821 gcggttctct tcgttgccat cgcctcgtgg gcactgacgg ccggcggcca gcagccgcag
     5881 agctacgtgc cgcggcaggt gatcatcaag ctggaggcgt cgacgccgac ggcggacgcg
     5941 ctcgcggtgc gatcgagcct gcgcgcgagt gtgaagcagc gcttcaagag cagcggggcg
     6001 gagttgtggg tgatcgatgg gatcgaggtc aacgaggcca tcgcgcgctt caaggacgat
     6061 ccgcgcgtcg attacatcga acccaactac acggtgcgcg cgctggacgt gttcccgaac
     6121 gatccccgct tcgacgatct gtggggcatg cacaacacgg ggcaaaccgg cggcaccccg
     6181 gacgcggaca tcgacgcgcc ggaggcgtgg gagctgggga cgggcggcgg cgtgctggtc
     6241 ggcgtgatcg acaccggtgt ggactacaac cacgtcgatc tggccgccaa catgttcatc
     6301 aacccgaacg aaatcccggg caacggcgtc gacgacgact tcaacggcta catcgacgac
     6361 gtgcgcggct gggacttcgt caacaacgac aacaatccca tggacgacca cgggcacgga
     6421 acgcacgtct ccggcaccat cggcgccctc ggcaacaacg gcgtcggcgt ggtgggtgtg
     6481 agctggtcgg tgcgcatcat ggggctcaag ttcctcgatg cgggcggatt cgggtcgacc
     6541 tcggacgcca tcctcgcggt cgagtacgcg accatgatgg gcgtcaagct caccagcaac
     6601 agctggggcg gcggcgggtt ctcgcaggcg ctgcaggatg cgatcgaggc cgccggcaac
     6661 gcgggcattc tgttcgtggc cgcggccggc aacgccagtt ccaacaacga cgtgttcccg
     6721 aacttcccgt cgaactatcc cctcgacaac atcatcgccg tggccagcac cgaccaccgc
     6781 gataacctga gcggcttttc caacttcggt ttgaccacgg tcgacctggg tgcgcccggc
     6841 accgacatct tgagcacgtt cccgaacaac acctacggga ccatctcggg cacgtccatg
     6901 gcgaccccgc acgttt
//
LOCUS       DASPGK010000144        11103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_507897, whole genome shotgun sequence.
ACCESSION   DASPGK010000144 DASPGK010000000
VERSION     DASPGK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507897"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..282
                     /locus_tag="VEC56_04155"
                     /pseudo
     CDS             109..282
                     /locus_tag="VEC56_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(287..1912)
                     /locus_tag="VEC56_04160"
     CDS             complement(287..1912)
                     /locus_tag="VEC56_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV13378.1"
                     /translation="MPRIPRPDPETVRERDALRRQVHALRAELEEYRIAQVPLRDTEQ
                     RLRALVEQAPIVVFAIDRDGIFTLSEGHGLVALGLRPGQVVGQSAYDVYRDVPRIVDN
                     VRRCLAGESINEVVQVGDLVFESIYTPRRDEHGFVAGVSGVAWDVTQRVRAETVAKEL
                     EIQLQQAQKIETIGTLAGGIAHDFNNILSPIIGYTEIAMDQLANDHPARADMEQVMNA
                     AIRAKELVQQILVFARGGSNERRPLQLHLVIHEALRLVRSTLPSTIEISQRVATRGDT
                     VVADPAQMHQVIMNLCTNAAHAMRESGGVLRIELVGEDVSETTAARVAGINPGPYVVL
                     TVRDQGTGMDAATMARVFEPFFTTKKSGEGTGLGLSVVHGIVHSHGGAVTVESDLGRG
                     SVFRVYLPSASAEAEEALRHGAAESRGQGEHVLVVDDEADVTQLLERILVARGYRVTS
                     FTSSEEALNAFRRDPASFRAVITDQTMPRLTGIELARQLKALRPGVAVILTTGYGENL
                     VSPPIGSGVDAIAAKPFDAAALAQTLRRTLTAE"
     gene            complement(1932..3998)
                     /locus_tag="VEC56_04165"
     CDS             complement(1932..3998)
                     /locus_tag="VEC56_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXV13379.1"
                     /translation="MGRSARSLYSVVRRVAALWAVEVGALWAMARVLPGLSLEGWSAA
                     VIGVAVIALLNALVRPLLLYLTLPFTVLSFGLLTLAINAVIVSIAALVVPGLHIAEFA
                     TGVVVALGLSVVNTVAASLLAFNDEDSVYRNIARRIARHRHPMPEGVAPGIVFVEIDG
                     LAFEALARATGDGHLPTLASWIRSGSHRVLRWDCGVPSQTSSSQAGILFGNNFDIPGF
                     RWYEKSTGKLVVSNDPSDAAHIEQRVSRGAGLLRLGGLSVCNMFTGDAEKNVAIISTF
                     SSPARQVRKTSSLYFSFYVNPYNFTRALVLMLREIVIERWEAFRQHARDLQPRVSRGG
                     SYPLLRAMSTVAQRELGTYTLMREMFAGVPSAYITYVGYDVVAHHAGPERPDALRILH
                     DLDRQVAILERAARDGPRPYRFVVLSDHGQTASIPFRQRHGTTIDQLVQSLVSKTRRV
                     RASTLDTEGWGHVNALLSEAISHDRLSGRAARRILRARTRGGYVELGNRRKREASHGD
                     VVVCSSGNLGLVYFDELADRLTLEALVADHPGLIEGLIAHEGIGFVMVRSHLHGPVAM
                     GRGGVHYLREHRVDGSDPLADYGPHAASQLMRLDGFPHCGDVVVNGRYHPSIDQVESF
                     EEMVGAHGGLGGAQTHGFLLYPSQWAVPEEEIANAEDVYQLFVRWRDTLASGADPSAA
                     RVDALQ"
     gene            complement(4017..4778)
                     /locus_tag="VEC56_04170"
     CDS             complement(4017..4778)
                     /locus_tag="VEC56_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV13380.1"
                     /translation="MRAPLHITYVGHATCLIEMDGVRLLTDPVLRDRVGHIRRQSSAV
                     DPSWSHELDAVLISHAHLDHLDLPSLRRLGRDVRLIVPEGSRDLLARNGFRRVEEVRP
                     GEWTRVGSIDIETTLATHDGTRFPFGPVAETVGFIVHGHRAVYFAGDTDIFPEMDGIH
                     DALDVALLPVWGWGPTLGDGHMNPRRAAESLTMLRPHTAIPIHWGTFAPLGMGWLRPR
                     FLIDPPYDFVEHANDLAPNVDVKIVHPGNRFRFLN"
     gene            5012..5371
                     /locus_tag="VEC56_04175"
     CDS             5012..5371
                     /locus_tag="VEC56_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV13381.1"
                     /translation="MKPRVLVVDDEPQIRSMLRESLTRAGFDVNEASDGKEAIDTLRK
                     SSFDVVVADILMPEKDGLEVIMYLQRESPLTKCVAISAPSNRVFLQSAQLLGATRVVE
                     KPFTAAEIEAAVREVLS"
     gene            complement(5325..5837)
                     /locus_tag="VEC56_04180"
     CDS             complement(5325..5837)
                     /locus_tag="VEC56_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV13382.1"
                     /translation="MNTYFFTREQFVDRPVAGVFSFFADARNLEFITPPWLRFHITTA
                     GPIRMERGARVDYRIRLRGVPVRWTSEILEWSPPYRFVDVQVRGPYRLWEHTHEFFEV
                     AGGTLVRDEVRYALPMGRLGEIAVRRFVAKDVARIFEYRRDALASRLARDARPLTTAP
                     HEPPLRSRRP"
     gene            complement(5888..6295)
                     /locus_tag="VEC56_04185"
     CDS             complement(5888..6295)
                     /locus_tag="VEC56_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXV13383.1"
                     /translation="MSILDTANAAGFTTLVAAVKAAGLEETLSGTGPFTVFAPTDEAF
                     KKLPEGALDGLLADKEALKNVLLYHVVMGDVKAADVVKLKSAKTVEGADVAIDAKDGV
                     KINDAKVVKADVVASNGVIHVIDTVLLPPAKKK"
     gene            complement(6533..7474)
                     /locus_tag="VEC56_04190"
     CDS             complement(6533..7474)
                     /locus_tag="VEC56_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXV13384.1"
                     /translation="MEHSKDRAAARHPIGVVASRTGLPQDLIRAWERRYGAVAPARGR
                     TDRRLYSDEDIDKLRMLRQAVSRGRRISDVARLPVAELEALVAEDRSASDAGSRVRAR
                     SRVSADSLLAEAIEALEALDRHRLENVLANAAVEMSGRTVRQDLIVPLLELIGARWEE
                     GSLRIVHEHLASTIVRTFMSASRNGNDRTNAPRIVITTPAGQNHELGALMTATVAEEA
                     GWNVYYLGPNLPAEEIAAAVRQLDAKAVALSVIYRDTEAHVVDEMKRLRRLIDSSIPV
                     FVGGRAVASIRSRLDEVGIKSPGDLQEFRAELQVISS"
     gene            7604..7825
                     /locus_tag="VEC56_04195"
     CDS             7604..7825
                     /locus_tag="VEC56_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HXV13385.1"
                     /translation="MKSLDIVSAILVVVGALNWGLVGLFQFDLVATLFGGQSAVTSRI
                     VYALVGIAGIFLATRLKSGLGVRTATQRA"
     gene            7851..8705
                     /locus_tag="VEC56_04200"
     CDS             7851..8705
                     /locus_tag="VEC56_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV13386.1"
                     /translation="MSRTSTMPRATRSTLDVYLNEIQRYPLLERARENELARRTRAGD
                     AIAREELIRSNLRFVVAIAKRYVGNGVALEDLVNEGNLGLVRAAERFDPERGHKFISY
                     AVWWIRQGILHAVSEHSRLVRLPMNRVALAQKATRAARQMEQHLGREAHPDEIASALN
                     VTADEIEEVAIFSRPHLSLDEPVGDGTDDLTFANQIEDTNSEQPDSALNAELANRSLR
                     RMLDGLGDRERTIISLYYGMESGEPLTLEQIGKRLGYTRERIRQIKEQAIEKLRVKAR
                     PLGDYASA"
     gene            complement(8858..9226)
                     /locus_tag="VEC56_04205"
     CDS             complement(8858..9226)
                     /locus_tag="VEC56_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV13387.1"
                     /translation="MSRIVHFEVPADNPERAQKFYAQALGWEFTAFGGPMPYWLVKTG
                     PTGAPGIDGGLLKRGAPGQGPMVIAGVASIDTSIEQVKKAGGTQVVDKMAIPGVGYAA
                     YFLDTEGNCFGIFQDDVNAK"
     gene            complement(9252..9608)
                     /locus_tag="VEC56_04210"
     CDS             complement(9252..9608)
                     /locus_tag="VEC56_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13388.1"
                     /translation="MNKIVLGLVVGGILGALDGLSALVSAPETQPQIVGIVIGSTIKG
                     IVAGLLIGWFARKVRSVPAGVAFGFFIGLVLAFLVALMPDAEGKHYWWQIMLPGSIVG
                     LIVGYATQRYGAVARA"
     gene            complement(9689..10687)
                     /locus_tag="VEC56_04215"
     CDS             complement(9689..10687)
                     /locus_tag="VEC56_04215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV13389.1"
                     /translation="MSHITSRAIARLREAVEFPDLAGTQYDAVEAIGRGGMGTVFRVH
                     DRELARDVALKVLSAREVSEDARARMLDEARILARLEHPGIVPVHDVGVLPDGRVFYT
                     MKLVRGSRLDEYARATHEIADLLRVFVRACEAVAFAHSEGVVHRDMKPSNVMVGAFGE
                     VLVLDWGVAKLLDRGTATETTTADAAAPSSSAPSTPPRRSADTATGAVLGTPGFMAPE
                     QARGESAHVDPRSDVYALGAMLRQILQASRAGAMPRRLRAICDRATAVDPAARYPDVA
                     SLSRDVERFATGQAVTAYKEHWTERARRLASRHRIAVTLIAAYLLMRVLIFFLDRP"
     gene            complement(10684..>11103)
                     /locus_tag="VEC56_04220"
     CDS             complement(10684..>11103)
                     /locus_tag="VEC56_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13390.1"
                     /translation="RGGGVDHVPLDFTTAEGELRELPVAGGADPEALFREEWVRRMFE
                     LAVEDLRGECESGGKQIHFALFERYDLDSPEAHESPESYHDLARRFDIPVTQVTNYLA
                     WARRRFREALLERVREVTSGEEEFRAEVRDLLGGFEP"
BASE COUNT         1948 a         3616 c         3722 g         1817 t
ORIGIN      
        1 gggaagcccg cggcgctcgc cgggtccgcc gcaggcacag tcgcttaaga aaggatcgtc
       61 atgcacgtat cgatcgagta ctgcatccct tgagactaca ctccccaggc cttcagtttg
      121 aaggccgcgc ttgaaaaggc attcccggat atccagatcg agctcatcaa agggaaaggc
      181 ggggcgttcg agattcgaag cggccagacg cttgtgttct ccaagaagaa atcggggcgt
      241 ttcccgagca acgacgagat cgttgccgcg ctgaagcgct agcgcgtcac tcggccgtga
      301 gcgtgcggcg cagcgtctgc gcgagcgcgg ccgcgtcgaa gggcttggcc gcgatcgcgt
      361 cgacgccgga gccgatgggc ggcgagacca gattctcgcc gtagccggtg gtgagaatca
      421 ccgcgacgcc gggacgcagc gccttgagct gccgcgccag ctcgatgccc gtcagtcgcg
      481 gcatcgtctg gtcggtgatg acggcgcgaa acgatgccgg gtcgcgacga aacgcgttca
      541 gcgcttcctc gctggacgtg aacgacgtca cacggtagcc gcgcgcgacc aggattcgct
      601 cgagcagctg ggtgacgtcg gcttcgtcgt ccacgaccag cacgtgctcg ccctgcccgc
      661 gcgactcggc ggcgccgtgg cgcagcgcct cctcggcctc cgccgaggcg cttggcaagt
      721 acacgcgaaa aaccgagccg cgcccgagat cgctctcgac cgtgaccgcg ccgccgtggc
      781 tgtgcacgat gccgtgcacc accgagagcc ccagtccggt tccctcgccc gacttcttgg
      841 tcgtgaagaa cggctcgaag acgcgcgcca tggtggcggc gtccatgccc gtaccttgat
      901 cgcgcacggt gaggacgacg tacggtcccg ggttgatccc ggccacgcgc gcggccgtcg
      961 tctcgctcac gtcctcgccc acgagctcga tgcgcagcac cccgccggat tcgcgcatcg
     1021 cgtgcgcggc gttggtgcac aagttcatga tgacctggtg catctgtgcg gggtcggcga
     1081 cgacggtgtc accgcgggtc gcgacgcgct gcgaaatctc gatggtggac gggagcgtgg
     1141 aacgaaccag gcgcagcgct tcgtgaatca ccaggtggag ctggagtgga cggcgctcgt
     1201 tgcttccgcc gcgcgcgaag acgagaatct gctggaccag ctccttcgcc cggatggccg
     1261 cgttcatcac ctgctccatg tccgcgcgcg cgggatgatc gttcgcgagc tggtccatcg
     1321 cgatctcggt atatccaatg atgggcgaca gaatgttatt gaagtcgtgc gcgatgcccc
     1381 ccgccagcgt cccgatggtt tctatcttct gcgcctgctg cagctggatc tcgagctcct
     1441 tggcgaccgt ctccgcccgc acgcgctggg tgacgtccca cgcgacgccc gagacgccgg
     1501 ccacgaaacc gtgctcgtcg cggcggggcg tgtagatcga ttcgaacacc agatcgccga
     1561 cctgcaccac ctcattgatg gactcgccgg ccaaacagcg acgcacgttg tcgacgatgc
     1621 gaggcacgtc gcggtagacg tcgtacgcgg actgccccac cacctgcccc ggcctcaatc
     1681 ccagcgccac gagaccgtgt ccctcggaca acgtgaagat gccgtcgcga tcgatcgcga
     1741 acacgacgat cggcgcttgc tcgaccaagg cccgcaagcg ctgctcggtg tcgcgaagcg
     1801 gtacctgggc tattcggtac tcttcgagct cggcgcgcag ggcgtggacc tggcggcgca
     1861 gagcgtcgcg ctcgcggacg gtttcggggt cgggacgagg gatgcggggc atgccgtcaa
     1921 ctatacggca ttcattgcag cgcgtccacg cgcgcggccg acggatcggc gccggacgcg
     1981 agcgtgtcgc gccagcgaac gaatagctgg taaacatcct cggcgttggc gatctcctcc
     2041 tcgggcacgg cccattgcga cggatacagc agaaagccgt gggtctgggc gccgcccagc
     2101 ccgccgtggg cacccaccat ctcctcgaag ctctccactt ggtcgatact cgggtggtag
     2161 cgcccgttga cgacgacgtc gccgcagtgc gggaacccgt cgagacgcat cagctgcgat
     2221 gcggcgtggg gtccgtagtc cgcgagcgga tcgcttccgt ctacgcggtg ctcgcgcaag
     2281 tagtggacgc cgccgcgtcc catcgcgacc ggcccgtgca agtgggagcg aaccatcacg
     2341 aagccgatcc cttcgtgggc gatcaagccc tcgatcaagc cggggtgatc ggcgaccaga
     2401 gcttcgagcg ttagtcgatc cgcgagctcg tcgaagtaga cgagccccag gtttcccgac
     2461 gagcacacga cgacgtcgcc gtgcgacgcc tcgcgcttcc tgcggttacc gagctcgacg
     2521 tagcccccgc gcgtgcgcgc gcgcaggatg cggcgggccg cgcgcccgct gagccggtcg
     2581 tggctgatcg cctccgacag gagcgcgttc acgtgccccc acccttcggt gtcgagcgtc
     2641 gaggcgcgca cccggcgtgt cttgctgacc agagattgta ccagctgatc gatggtagtc
     2701 ccgtggcgct gtcggaaggg aatgctcgcg gtttggccgt ggtccgacag cacgacgaat
     2761 cgatagggtc gtggcccatc gcgggcggcg cgctccagaa tggccacctg gcggtcgagg
     2821 tcgtggagga tgcgcaacgc atcggggcgc tcggggccgg cgtggtgcgc gaccacgtcg
     2881 tagccgacgt acgtaatgta cgcgctgggc acgccggcga acatctcgcg catgagtgtg
     2941 taggttccca attcacgctg ggccaccgtc gacatagcgc gcaggagcgg gtagctcccg
     3001 ccgcgcgaga cgcgcggctg caggtcgcgc gcgtgctgcc gaaacgcctc ccatcgctcg
     3061 atgacgatct cgcgcagcat gagcacgagc gcgcgtgtga agttgtaagg attcacgtag
     3121 aacgagaagt agagcgacga cgtcttgcgg acctgccgcg cgggcgagga gaacgtgctg
     3181 atgatggcca cgttcttctc ggcgtctccg gtgaacatgt tgcacacgct cagcccaccc
     3241 agccgcaaga ggccggctcc gcgcgacacg cgctgctcga tgtgcgccgc gtcggagggg
     3301 tcgttcgaca ccaccagctt gccggtggac ttttcgtacc agcggaaccc gggaatgtcg
     3361 aagttgttgc cgaagagaat cccggcctgg ctcgacgacg tctgcgaggg aaccccgcaa
     3421 tcccagcgca gcacgcggtg cgatccgctt cgaatccacg acgcgagcgt gggcaggtgt
     3481 ccgtcgccgg tggcgcgcgc cagggcctcg aaggcgagac cgtcgatctc gacgaagacg
     3541 atcccggggg ccacgccctc gggcatcggg tggcggtggc gcgcgatccg gcgcgcgatg
     3601 ttgcggtaca ccgagtcttc gtcgttgaac gcgagcagcg atgccgcaac cgtgttgacc
     3661 accgagagcc cgagtgcgac caccacgccg gtcgcgaatt cggcgatgtg caacccgggc
     3721 acgaccaggg ccgcgatcga tacgatgacg gcgttgatcg cgagggtgag cagcccgaag
     3781 gagagaaccg tgaaggggag cgtgaggtag aggagcaggg gacgcacgag cgcgttcaag
     3841 agagcgatca ccgccacccc gatcacggcc gcgctccaac cttccaagga gaggccgggc
     3901 aacacgcggg ccatggccca gagggcgccg acctcgacgg cccatagcgc tgccaccctc
     3961 cggaccaccg agtagaggga ccgagccgaa cgacccacgg cctcgattat tgccgattaa
     4021 ttgaggaaac ggaaccggtt accggggtgc acgatcttca cgtcgacgtt gggagccaaa
     4081 tcgttggcgt gctcgacgaa gtcgtagggg ggatcgatca ggaaacgcgg gcgcagccag
     4141 cccatcccga gcggagcaaa cgttccccag tggatgggaa tcgcggtgtg cgggcgcaac
     4201 atcgtcagcg attcggcggc ccggcgcggg ttcatgtgcc cgtcgccgag ggtcggaccc
     4261 caaccccaga ccggcagcag agcgacgtcg agcgcgtcat ggatgccgtc catctcgggg
     4321 aagatgtcgg tatcgccggc gaaatagacg gcgcgatggc cgtgcacgat gaagccgacg
     4381 gtttcggcca cgggaccgaa ggggaagcgc gtcccgtcgt gggtggcgag cgtcgtttcg
     4441 atgtcgatcg agccgacccg cgtccactcg ccaggccgga cttcttcgac gcgacggaag
     4501 ccgttgcgag ccaggaggtc gcggctgcct tccggaacga tgaggcgcac gtcgcgcccc
     4561 aggcgacgca aggacggcaa gtcgagatgg tccagatggg cgtgcgagat cagcaccgcg
     4621 tcgagctcat gcgaccacga cgggtcgacg gcgctggact ggcggcgaat atgcccgacg
     4681 cgatcgcgga gaacggggtc ggtcaggagg cgcacgccgt ccatctcgat caagcaggtt
     4741 gcgtgtccga cgtaagtaat atggagcggc gctcgcatgc gtcgatattt ctgcaaggac
     4801 ccggccaagc ggttggtggc ggcgggatgg gaacggttgg ggtggtctct tcctgtttgg
     4861 ggacttgccg gcaatataac aggcgcgatg cggttcgcaa gagacaactc cggggaacac
     4921 cggaaatgcc ctccgcgaat cccccttcca aagccgacta ccggagcata gactctgtta
     4981 ctggttaggg ccctggagga aggagggaga catgaagccc cgtgtcctgg tagtggacga
     5041 cgagccgcag atccgcagca tgctgcgcga gtcgctcacc cgtgccggtt ttgatgtgaa
     5101 tgaagcctcc gacggcaagg aggcgatcga tacgcttcgc aagagttctt tcgacgtcgt
     5161 cgtcgcggac atcctgatgc ccgagaaaga cggcctcgaa gtcatcatgt acctccagcg
     5221 cgaaagcccc ctcaccaagt gcgtcgccat cagcgccccg agtaaccgcg tcttcttgca
     5281 gagcgcccag ctcttgggag cgacgcgcgt cgtcgaaaag cctttcacgg ccgccgagat
     5341 cgaagcggcg gttcgtgagg tgctgtcgtg agcggtcgcg cgtctcgcgc caagcgagag
     5401 gcgagcgcgt cgcgccggta ttcgaagatg cgcgccacgt ctttcgcgac gaatcgccga
     5461 actgcgatct cgcccagtcg tcccatgggc agcgcgtagc ggacttcgtc gcggacgagc
     5521 gtacctcccg cgacctcgaa gaactcgtgg gtgtgttccc acagccgata cgggccgcgc
     5581 acctggacgt ccacgaagcg gtacggtgga ctccactcca ggatctcgct cgtccaccgg
     5641 accggcacgc cgcgcaggcg gatgcgatag tcgacgcgcg ctccgcgctc cattcgaatc
     5701 ggtcccgcgg tcgttatgtg gaaccgcaac catggcggag tgatgaactc gaggttgcgt
     5761 gcatccgcga agaacgagaa cacacctgcc acgggacgat cgacgaactg ctcgcgggta
     5821 aagaagtatg tgttcatatg ggcctgaccg ggcagctcgg cttcgaattc cgagctgccc
     5881 agccgagtta cttcttcttc gccggcggca ggagcacggt gtcaatgacg tgaatgacgc
     5941 cgttgctcgc gacgacgtcg gccttgacga ccttggcgtc gttgatcttg acgccgtcct
     6001 tggcgtcgat cgccacgtcc gcgccctcca cggtcttcgc cgacttcagc ttgacgacgt
     6061 cggctgcctt cacgtcaccc atgacgacgt ggtagagcag gacgttcttg agcgcttcct
     6121 tgtcggccaa cagtccgtcg agtgcgccct ccggaagctt cttgaaggct tcgtcggtag
     6181 gggcgaacac ggtgaacggc cccgtgcccg agagcgtctc ttccaagccg gcggccttga
     6241 ccgcggcgac gagcgtggtg aagccggccg cattcgcggt gtcgaggatc gacatcggtg
     6301 tggtggcgga cgtcgctttg gccgccttct gctgggtatc gccggccgac gccgagagca
     6361 cgaggccgaa gcctatcgag atcgcggcga gcaccgccgt aagtttgcga ttcatggttt
     6421 cctcctagtt gaattgcatc tttcattcga gtccgaagtg gtggtcgact cgagcggagg
     6481 gaacgtacga acacgcatgg cgcgtgtcaa aactcgcgat cgaattggag ccctaagacg
     6541 agattacttg cagctcggcg cggaattcct gcaggtcgcc gggcgacttg atgcctactt
     6601 catcgaggcg actgcgaatc gaagccacgg cgcgacctcc gacgaacacg gggatcgatg
     6661 agtcgatcaa acgacgcagt cgtttcatct cgtcgacgac gtgggcttcg gtgtctctat
     6721 aaatgacgct caacgcgacc gccttggcgt ccagctggcg gacggcggcc gcgatttcct
     6781 ccgcggggag attgggcccc aagtaataga cgttccagcc ggcttcctcg gcgacggttg
     6841 cggtcatcaa cgcgccgagc tcgtggttct gtcccgcggg agtcgtgatg acgatgcgag
     6901 gcgcgttggt tcgatcgttc ccgttgcgcg acgccgacat gaacgttcgc acaatcgtgc
     6961 tggcgaggtg ctcgtgcacg atgcgcagcg acccttcctc ccaccgagcg ccgatcagtt
     7021 ccagcagcgg cacgatgaga tcctgacgaa cggttcggcc gctcatctcg accgccgcgt
     7081 tcgcgagcac gttctctaag cgatgccggt cgagcgcttc gagcgcttcg atggcctcgg
     7141 cgagcagcga gtccgccgaa acgcgagagc gcgcgcggac ccgagatccg gcgtcggacg
     7201 cggagcgatc ctcggccacc agagcctcga gctcggcgac ggggagtcgc gcgacgtcgc
     7261 tgatgcgacg accgcgcgag accgcctgcc ggagcatgcg gagcttgtcg atgtcttcgt
     7321 cggagtagag gcgccggtcg gtgcgaccgc gggccggggc gacggcgccg tagcgacgct
     7381 cccacgcccg gatcaggtcc tgggggagcc cggtccggga ggccaccacg ccgatcggat
     7441 gtcgtgcagc ggcacggtct ttcgagtgtt ccacgccccc agcttacgga cgtgtcccat
     7501 ctttgtcaag gaaatgacca aaattacgct tgacgagctg gcttctgtac atgtattgtc
     7561 ttggacatca agttggcaat tgtccaaacc aaggaggaac accatgaaga gcctcgacat
     7621 tgtctcagcg attctcgtcg tcgtgggtgc gctcaactgg ggcctcgtcg gcctgttcca
     7681 gttcgacctc gtcgccacgc tgttcggggg tcagtcggcc gtgacttcac ggatcgtgta
     7741 cgcgctcgtc ggcatcgcgg gcatcttcct cgccacccgc ttgaagtcgg ggctcggggt
     7801 ccgcaccgca acccagcgcg catagcgcgc cgtgagagga cgtgatcgac atgagccgca
     7861 cttcgacgat gccccgcgcg acccgctcga cgctcgacgt gtacttgaac gagatccagc
     7921 gctatccact cctcgagcgg gcgcgagaga acgagctcgc gcgcaggacg cgcgcgggcg
     7981 acgccatcgc gcgtgaggag ctgatccgct ccaacctccg tttcgtggtc gcgatcgcga
     8041 agcgctacgt cgggaacggg gtggcgctcg aggacctggt caacgaaggg aacctcggac
     8101 tcgtgcgggc ggcggagcgc ttcgacccgg aacgcgggca caagttcatc tcgtatgcgg
     8161 tgtggtggat tcggcagggg attctgcacg cggtatccga gcattcgcgc ttggtgcgac
     8221 tgccgatgaa ccgtgtcgcg ctcgcccaga aggcgacgcg ggcggcgcgc cagatggagc
     8281 agcacctggg ccgcgaagca caccccgatg agatcgcgtc cgcattgaac gtcaccgccg
     8341 acgagatcga agaagtcgcg atcttctcgc gccctcatct ctcactcgac gagcccgtcg
     8401 gcgacggcac ggacgacctg acgttcgcca atcagatcga agatacaaac tcggagcaac
     8461 ccgactcggc gctcaacgcg gagcttgcga accgcagctt gcgacgcatg ctggacggac
     8521 tcggcgaccg cgagcgcacg atcatctcgc tgtattacgg gatggagagc ggcgagccgc
     8581 tgacgctgga gcagattggc aagcgcctgg gatacacgcg cgagcgcatt cggcagatca
     8641 aagaacaagc catcgagaag ctgcgcgtga aagccaggcc cctcggcgac tacgctagcg
     8701 cctagtggaa cacgagcggg ggccggtcgc acgatcgaga acgagtcagt caacgaggag
     8761 cgagtgcctc gattgggcgt gccgactccc gcagaaccga agaagacccc ggtggacgag
     8821 tccgccgggg tcttctcttg tcgatgcggc ggcgacgcta cttcgcgttg acgtcgtcct
     8881 ggaagatccc gaaacagttg ccttcggtat cgaggaagta ggccgcatag cccactcccg
     8941 ggatcgccat cttgtcgacc acttgggtcc cgccggcctt tttcacctgt tcgatcgacg
     9001 tgtcgatcga cgcgacgccg gcgatcacca tcgggccttg gccgggcgcc ccgcgcttga
     9061 gcagacctcc gtcgatcccc ggcgccccgg tcggcccggt cttgacgagc cagtacggca
     9121 tcggcccgcc gaacgcggtg aactcccatc cgagggcttg cgcgtagaac ttctgcgcgc
     9181 gctcgggatt gtcggcggga acttcgaaat gaacgattcg cgacatggaa cgtctccttt
     9241 tccgttgagg gctacgcgcg cgcgaccgcg ccgtagcgtt gtgtggcgta cccgacgatg
     9301 aggccgacga tgctcccggg cagcatgatc tgccaccaat agtgtttccc ctccgcgtcc
     9361 ggcatcaggg ccacgaggaa cgccagcacc agaccgatga agaacccgaa tgcgacgccc
     9421 gcgggaaccg agcggacctt gcgcgcgaac cagccgatca ggagaccggc gacgatcccc
     9481 ttgatggtcg atccgatgac gatgccgacg atctgcggct gggtttccgg cgcggaaacc
     9541 agcgcggaga gcccatcgag ggcgcccaag atgcctccca ctacgagccc caacacgatt
     9601 ttgttcatgt ccgtcctttc tttagccctt gcccgggccc ttcggccctc actctccatt
     9661 caacgattcg ggggattcct ttaacacgtc agggacgatc gaggaaaaaa atcagcaccc
     9721 gcatcaacag gtaggcggcg atcaaggtaa cggcgatgcg gtggcgcgac gccagccgcc
     9781 gggcccgctc ggtccagtgc tctttatagg cggtgaccgc ctgtccggtg gcgaaccgct
     9841 cgacgtcccg ggacaaggac gcgacgtccg ggtagcgcgc ggccgggtcg acggcggtcg
     9901 cgcggtcaca gatcgcgcgc aaccggcgcg gcatcgcacc cgcgcgcgat gcttgcagga
     9961 tctgccgcag catcgcgccc agcgcgtaca cgtcgctgcg cggatcgacg tgggccgatt
    10021 cgccgcgcgc ctgctcgggc gccatgaagc cgggagtccc gagcaccgcg ccggtggcgg
    10081 tgtcggcgct tcgccgcggc ggcgtcgacg gggccgacga cgacggcgcc gccgcgtcgg
    10141 cggtggtcgt ctcggtggcc gtgccgcgat cgaggagttt cgcgacaccc cagtccagga
    10201 ccagcacttc gccgaaggca cccaccatca cgttgctcgg cttcatgtcg cgatgaacca
    10261 cgccctccga gtgcgcgaag gcgacggctt cgcacgcgcg cacgaacacg cgcaggagat
    10321 cggcaatctc gtgcgttgca cgcgcgtact catcgaggcg gcttccgcgc acgagcttca
    10381 tggtatagaa gacacggccg tcggggagca cgccgacgtc gtggacgggg acgatgcccg
    10441 ggtgttcgag gcgcgcgagg atgcgcgctt cgtccagcat gcgcgcgcgc gcgtcttcgg
    10501 aaacctccct cgcgctcaac accttgagag ccacgtcgcg cgccagctcg cggtcgtgga
    10561 cgcgaaacac cgtccccatg ccgccgcgtc cgatggcctc gaccgcgtcg tactgggtgc
    10621 ctgcgaggtc ggggaactcg accgcctcgc gcaagcgggc gatggcacgc gaggtgatgt
    10681 ggctcacggc tcgaagcctc ccaggaggtc gcgcacctcg gcgcggaact cctcttcgcc
    10741 ggaggtcact tcgcgaacgc gctcgagcag ggcctcgcga aaccgccgcc gcgcccacgc
    10801 gaggtagttg gtcacctggg tgaccggaat gtcgaaacgg cgcgcgaggt cgtggtatga
    10861 ctcgggggac tcgtgtgcct ccggcgagtc caaatcgtag cgctcgaaga gcgcgaaatg
    10921 gatctgcttg cctccggact cgcactcgcc gcgcaggtcc tcgacggcca gctcgaacat
    10981 gcgtcgcacc cattcctcgc ggaacagcgc ctcgggatcc gcaccgcccg cgacggggag
    11041 ctcgcgcagc tcgccctcgg cggtcgtgaa gtcgagcggc acgtggtcaa caccaccgcc
    11101 gcg
//
LOCUS       DASPGK010000145         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1210626, whole genome shotgun sequence.
ACCESSION   DASPGK010000145 DASPGK010000000
VERSION     DASPGK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210626"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VEC56_04225"
     CDS             <1..525
                     /locus_tag="VEC56_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13391.1"
                     /translation="YVFVGNVFTDVLTARGVTIIAPRAASAPAMPAVGGANPMVASPP
                     GSDGSGGDPQSPSSDTVVLTFQNTAFAIAYADVYRSHLIGGKRVKRRADVRVHATVTE
                     AGSGRVLWVGEAFRGSADEFDYEDSARVEQGTYQFARPVLPGSGWGKYAEPVFVTGII
                     VGLIYLFFSNQSDN"
     gene            515..1438
                     /locus_tag="VEC56_04230"
     CDS             515..1438
                     /locus_tag="VEC56_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13392.1"
                     /translation="MTTSARGRVRWAAIPLGIAWLVCGLSACGERGVVGEMHRAFDQG
                     DYDEVMALGRHALRSDSSSAGVHLYYGMALVARGRLHEGFGAIDRAVAIDADAARKAS
                     EFLWERGEAAKLDASTARELAKASELDPSLDLGKKRFAVGDTYFSERRYPEAAGMYEA
                     AVRLYPDTAACERALSQLAECYAFLDRPADARKTMETLVKRYPRGDVARQAWVRLDDI
                     SYAEAQKALEAGELEEAVEIAQGLVDRTKNRSLQQKGRLVLGEAYERMGETGRAYTAY
                     RDVLENDRGDSGNVVERARARIEALQAAGLR"
     gene            1444..2199
                     /gene="bamD"
                     /locus_tag="VEC56_04235"
     CDS             1444..2199
                     /gene="bamD"
                     /locus_tag="VEC56_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXV13393.1"
                     /translation="MPAARVSRRAGVSLALVILAGCVGGMPKVPATPTAVLERADDYH
                     ARGKEREAIALYTQFLDRYPGHDRADYAQFQLAECQFKARDYALAAVEYQILISNYGY
                     SEWVDDALFQMGVCNWREAPKYPRDQQKSTEALSRFNQFLQIYPDSPRASEARAYIRE
                     IHARLAQKAYSAAKWYYRQREPKAALVYCEKIIREYPDNEYWAEALLLKGQILIDRGD
                     TEGAIQQFTQIIAYPGELPQKKEAEHRIKEARP"
     gene            2196..2804
                     /gene="nadD"
                     /locus_tag="VEC56_04240"
     CDS             2196..2804
                     /gene="nadD"
                     /locus_tag="VEC56_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HXV13394.1"
                     /translation="MTPDPSEDWKGNRIGILGGTFDPPHVGHTRMAAAARDALGLDRV
                     FCSPAPHPPHKSDAETTSYRHRRAMLEFAIAGEDRVAVTGIEESHATSYTVDLLRACR
                     ERTSADLYFIMGADSLAGLPTWRDPADVMRLATLVVFPRGREIVRAPDAREVSLVVFE
                     SPVIDVSSTEIRERLARGETSVGGLAPAVARYVAHHRLYARG"
     gene            2809..>4066
                     /locus_tag="VEC56_04245"
     CDS             2809..>4066
                     /locus_tag="VEC56_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXV13395.1"
                     /translation="MALFLVDGHALAYRAYFAFAGTPLSNSRGEETGAVYGFVNTLLS
                     IIVKHKPEQLVVAFDSLEPTFRHERFEAYKAHRPRMPEGLIRQLPVIFEAVDAMGIPR
                     LQAPGYEADDIIATLAHRFRERVPVAIVSGDKDLLQLVDERVHVIRPGKGAVLENELD
                     PAGLEEKTGLRAGQFIDYLALVGDTSDNVPGVRGIGEKTATELLQRFGSLDEIYRRLD
                     EIEKPSVRARLEEGRDAALESRELVRLRDDVPLEVSLLDLARGDHRTPAFAAILHRLE
                     FKRMHDQMFGAGSPPAPRPRDPDPPARATERARTPEVTTHYRAIADAASLASLARDLA
                     ARDEIAVDVETSGLDPMRATLAGIAIARSPGDAVYVAIASEIDDGSSATLPGLLPASR
                     APGLDLGAVRTALAPLLAAGRPAKIGQ"
BASE COUNT          655 a         1391 c         1417 g          603 t
ORIGIN      
        1 tatgtcttcg tgggcaacgt cttcaccgac gtgctgaccg cgcgcggcgt gaccatcatc
       61 gcgccccgcg ccgcgagcgc gcccgccatg ccggcggtcg gcggcgcgaa cccgatggtc
      121 gccagtccac cgggaagcga cggatcgggc ggcgacccgc agagtccgtc gtcggacacc
      181 gtcgtcctga cctttcagaa caccgccttc gccatcgcgt acgccgacgt gtaccgctcc
      241 cacctgatcg gcggcaagcg cgtcaagcga cgggccgacg tgcgcgtgca cgccaccgtg
      301 accgaggcgg ggtcggggcg ggtgctgtgg gtcggtgagg cgttccgcgg gagcgcggat
      361 gaattcgact atgaggactc ggcgcgcgtc gagcagggta cgtaccaatt cgcgcgaccg
      421 gtgctcccag gaagcggttg gggcaagtac gcggagcccg tgttcgtcac cggtatcatt
      481 gtcgggctga tctacctctt cttttccaac cagagtgaca actagcgcgc ggggcagggt
      541 tcgctgggcg gcgatcccgc tggggatcgc gtggctggtg tgcgggctgt cggcgtgcgg
      601 cgagcgcggc gtggtcggcg agatgcaccg ggccttcgac cagggcgact acgacgaagt
      661 gatggcgctg ggtcggcacg cgctgcgctc ggactcgagc agcgcggggg tccatctcta
      721 ttacggaatg gcgctggtcg cgcgcggccg cttgcacgag ggctttggcg ccatcgaccg
      781 cgcggtcgcg atcgacgccg acgccgcgcg caaggcctcc gagtttctgt gggaacgcgg
      841 cgaggccgcg aagctcgatg ccagcactgc gcgcgagctc gcgaaagcat cggagctcga
      901 tccctccctc gacttgggca agaagcgatt cgcggtcggc gacacgtact tcagcgaacg
      961 ccgctatccg gaggcggcgg gcatgtacga agcggccgtt cgcctgtacc cggatacggc
     1021 cgcgtgcgag cgcgcgctct cgcagctggc cgagtgctac gccttcctcg atcgacctgc
     1081 ggacgcacgc aagaccatgg agaccttggt caagcgctac ccgcgcggcg acgtcgcgcg
     1141 ccaggcgtgg gtgcgcctgg acgacatctc ctacgcggag gcccagaagg cgctcgaggc
     1201 cggcgagctg gaagaagcgg tcgagatcgc gcaagggctg gtggaccgca ccaagaatcg
     1261 gtcgctgcag cagaaggggc gcctggtgct gggcgaggcg tacgagcgca tgggcgaaac
     1321 cggccgcgcc tacacggcct atcgcgacgt gctcgagaac gaccgcggcg attcgggcaa
     1381 cgtagtcgag cgcgcgcgcg cacgcatcga agcgctgcag gcggcggggc tgcgttgagc
     1441 acgatgccgg cggcgcgggt gtcgcgacgc gcgggagtct cgctcgcgct ggtcatcctc
     1501 gcggggtgcg tgggcggaat gccgaaagtc ccggccacgc ccacggccgt gctggagcgc
     1561 gccgacgact accacgcgcg cggcaaggag cgtgaggcga tcgcgctcta cacccagttt
     1621 ctcgaccgct acccgggcca cgaccgcgcc gattacgcgc agttccagct cgccgagtgc
     1681 cagttcaagg cgcgagacta cgcgctggcc gcggtcgagt atcagatcct gatcagcaac
     1741 tacggctaca gcgagtgggt cgacgacgcg ctgtttcaga tgggcgtgtg caactggcgc
     1801 gaagcaccca agtacccgcg cgaccagcag aagtcgaccg aggcgctgag tcgtttcaat
     1861 caattcctgc agatctatcc ggacagcccg cgcgcatcgg aagcgcgcgc gtacatacgc
     1921 gagatccacg cccgcctcgc gcagaaggcg tacagtgcgg ccaagtggta ctatcgccag
     1981 cgcgagccca aggcggcgct ggtgtactgc gagaagatca ttcgcgagta tccggacaac
     2041 gagtattggg ccgaggcgct gctcttgaag ggacagatct tgatcgaccg gggcgacacc
     2101 gagggcgcga tccagcaatt cacgcagatc atcgcgtacc cgggggagct tccgcagaaa
     2161 aaggaagccg aacaccgcat caaagaggcg cgcccatgac gccggaccca agcgaagact
     2221 ggaaagggaa tcgcatcggg attttgggcg gcaccttcga tccgccgcac gtggggcaca
     2281 cgcgcatggc ggccgccgcg cgcgacgcgc tcggcctcga tcgcgtgttc tgttctccgg
     2341 cgccgcaccc gccccacaag tccgacgcgg agacgacgtc gtatcgccat cgccgggcca
     2401 tgctcgagtt cgcgatcgcg ggcgaagatc gcgtcgccgt gacagggatc gaagagtccc
     2461 acgccacgtc gtacacggtc gatctcttgc gcgcatgccg cgagcgaacc tctgcggacc
     2521 tctatttcat catgggcgca gacagcctgg ccggattgcc gacctggcgc gaccccgcgg
     2581 acgtgatgcg gctggccacg ctggtggtct ttccgcgcgg gcgcgagatc gtaagggcgc
     2641 cggacgcgcg cgaggtctcg ctggtggtct tcgagtcgcc cgtgatcgac gtttcctcca
     2701 ccgagattcg cgagcggctc gcgcggggcg agacctccgt cggcgggctc gcgcccgcgg
     2761 tggcccgtta cgtcgcgcat caccgcctct acgcgcgggg gtagcgcggt ggccctcttc
     2821 ctcgtcgacg gacacgcgct cgcgtaccgc gcctacttcg cgttcgcggg caccccgctg
     2881 tcgaactcgc gcggggagga gaccggcgcg gtctacggct tcgtcaacac gctgctttcc
     2941 atcatcgtta agcacaagcc cgagcagctg gtggtcgcgt tcgacagcct ggagcctacc
     3001 tttcgccacg aacgcttcga agcctacaag gcgcaccggc cacggatgcc cgaggggctg
     3061 atccgccagc tccccgtcat cttcgaagcg gtggacgcca tgggcatccc gcgcctgcaa
     3121 gcccccggat atgaagccga cgacatcatc gccactctcg cgcatcgctt tcgcgagcgg
     3181 gttccggtgg cgatcgtctc cggcgacaag gatctcctgc agctggtcga cgagcgcgtg
     3241 cacgtgattc gccccggcaa gggcgcggtg ctggagaacg agctggaccc cgcaggcttg
     3301 gaggagaaga ccggcctgcg cgccggtcag ttcatcgact acctggcgct ggtgggggac
     3361 accagcgaca acgtccccgg cgtgcgcggc atcggcgaaa agaccgccac cgagctcttg
     3421 cagcggttcg gttcgctcga tgaaatctac cggcggctgg acgagatcga aaaaccgtcg
     3481 gtgcgcgcgc gcctcgaaga gggaagggat gcggccctcg agtcgcgcga gctggtacgc
     3541 ctgcgcgacg acgttccgct ggaggtttca ctcctggatc tcgcgcgcgg cgaccatcgc
     3601 acaccggcgt tcgccgccat cctgcaccgc ctggagttca agcgcatgca cgaccagatg
     3661 ttcggcgcgg gctcaccgcc cgcgccgcgc ccgcgcgacc cggacccgcc cgcacgcgcg
     3721 acggagcggg cgcgcacccc cgaggtcacg acgcactacc gcgcgatcgc cgacgcggcg
     3781 tcgctggcct cgctggcccg cgacctggcc gcgcgcgacg agatcgcggt cgacgtcgag
     3841 acgtcgggtc tggatccgat gcgcgcgacc ctggccggca ttgcgatcgc gcggtcgccc
     3901 ggcgacgcgg tctacgtcgc catcgcgagc gagatcgacg acggctcttc cgcgacgctg
     3961 cccgggttac tcccggcctc gcgcgcgcct ggactcgacc tcggcgcggt acgcaccgcg
     4021 ctggcccccc tcttggccgc cggacgcccg gccaagatcg gccaga
//
LOCUS       DASPGK010000146         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_742057, whole genome shotgun sequence.
ACCESSION   DASPGK010000146 DASPGK010000000
VERSION     DASPGK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742057"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VEC56_04250"
     CDS             complement(<1..511)
                     /locus_tag="VEC56_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV13396.1"
                     /translation="MDKNFLKGRGSVSNPTGRFERLAYDDGDADVVQPDEFAEDAPAR
                     PPTEFYRDSSSSIVARNQSPDVGFDASINPYRGCEHACIYCYARPTHEFLGLSAGLDF
                     ETKIFVKEKAPELLRAKLSSRSWKPTPLALSGVTDPYQPIERRLRLTRRVLEVLAEFK
                     NPVIVVTKNH"
     gene            575..1738
                     /locus_tag="VEC56_04255"
     CDS             575..1738
                     /locus_tag="VEC56_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV13397.1"
                     /translation="MPRTVWVLFVGTLINRFGSFVFVFLAVYLTGKGYTAPQAGLAIG
                     AYGVGAICASLVGGWLADHLGRRNTIVLSMLLSASVMVGLSQAAGYALLMTLTVLAGF
                     CAEMYRPAASALIADVTTPAQRVTAYALYRLAINAGFAIGPAVGGFMASKSFFFLFLG
                     DAVTSLLYAVIAIAALPNRVVEHHATHPRRSALRVVLNDSKFLLFLGSTMAASMVFMQ
                     HVSSYPLWITSLGFSSAVFGMLISLNGVLICLTELPLTSYTRRLPARRVMVIGMLVFG
                     TGFAATGLGRSIPFLAATVIVWTIGEMFYFPMAAAHVANISPPDMRGRYHGAWGISWG
                     LGAVVGPVLGTALFAVAPRAVWPVCGVVAVLGALLLLPATREHRAARAVTEAP"
     gene            complement(1751..2179)
                     /locus_tag="VEC56_04260"
     CDS             complement(1751..2179)
                     /locus_tag="VEC56_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXV13398.1"
                     /translation="MEWLQKEIVLAPRARGFHLVTDEVVRAIPELARFRVGVAHFFIQ
                     HTSASLCINENADATVRTDLERSFNELVPEDAPYYRHTTEGPDDMPAHVKAAMIGTSV
                     SVPITNGRLNLGTWQGLYLCEHRNRGGSRRVVATISGETV"
BASE COUNT          328 a          707 c          765 g          437 t
ORIGIN      
        1 ggtggttctt cgtcacgaca atgacggggt tcttgaactc ggcgaggact tcgagcacgc
       61 ggcgcgtcaa tctcaagcgg cgttcgatcg gctgatacgg atcggtgacg ccggagagcg
      121 ccagcggggt gggcttccac gagcgcgacg acagcttcgc gcgcaagagc tcgggtgcct
      181 tctccttgac gaagatcttg gtttcgaaat cgagacccgc ggacaggccc aggaattcgt
      241 gcgtcgggcg cgcgtaacag tagatgcacg catgttcgca gccacgatac ggattgatgc
      301 tggcgtcgaa gccgacgtcg gggctctggt tgcgcgcgac gatcgaggac gacgagtcgc
      361 ggtagaactc ggtgggcggg cgcgcgggcg cgtcctcggc gaactcgtcc ggttgcacga
      421 cgtcggcatc gccgtcgtcg taggcgagcc gctcgaagcg cccggtcgga ttggaaacgc
      481 tgccgcgacc cttgaggaag tttttgtcca ttgacgcctc cggccgtgta gtgtaggcca
      541 ccttgctatg tcgttgagaa aagatatcgc ctccatgccc cggaccgtgt gggtgctgtt
      601 cgtcggcacc ctgatcaacc ggttcgggag cttcgtcttc gtctttctcg ccgtctacct
      661 gacgggcaag gggtacacgg caccccaggc cggcctcgcg atcggggcct acggcgtggg
      721 tgcgatctgc gcctcgctgg tgggcgggtg gctggccgac catttgggcc gccgcaacac
      781 catcgtgctc tcgatgctgc tctcggccag tgtgatggtg gggctctcgc aggccgcagg
      841 ctacgcgctg ctcatgacgc tgaccgtcct ggccggcttc tgcgccgaga tgtaccgccc
      901 cgcggccagc gctctcatcg ccgacgtcac caccccggcg cagcgcgtca ccgcctacgc
      961 gctctaccga ttggcgatca acgccggctt cgcgatcgga cccgcagtgg gcggattcat
     1021 ggccagcaag tcgttcttct tcctgttcct cggcgacgcg gtgacgtcgc tactctatgc
     1081 cgtcattgcc atcgccgcgc tccccaatcg cgtggtcgag caccacgcga cgcacccgcg
     1141 acgcagcgcg ctgcgcgtgg tccttaacga ctcgaagttc ctcctgttcc tgggatcgac
     1201 catggccgcg tcgatggtct tcatgcagca cgtgtcgagc tatcccttgt ggatcacgtc
     1261 gctggggttc tcgagcgccg tgttcggcat gctgatctcc ctgaacggcg tgctcatctg
     1321 tctgaccgag ctgccgctga cgtcgtacac gcgcaggctg ccggcgcggc gcgtgatggt
     1381 gatcgggatg ctggtgttcg ggacgggctt cgcggcgacc gggctcggga ggtcgatccc
     1441 gttcctggct gccaccgtga tcgtctggac gatcggcgag atgttctatt ttcccatggc
     1501 cgcggcgcac gtcgccaaca tttcgccgcc cgatatgcgc gggcgctatc acggggcgtg
     1561 gggaatttcg tggggattgg gggcggtggt gggaccggtg ttgggcacgg cactcttcgc
     1621 ggttgcgccg cgcgcggtgt ggccggtgtg cggggtggtc gcggtgctgg gagcactcct
     1681 cctactgccg gcgacgcgcg agcaccgcgc ggctcgtgcg gtgacggagg cgccgtagcg
     1741 cgcccagaaa ctacactgtc tcgccgctga tggtcgctac cacgcgacgc gaaccgcctc
     1801 ggttgcggtg ttcgcacaga tagagcccct gccacgtgcc cagattcaag cgaccgttcg
     1861 taatgggaac gctcacactc gtgccaatca tggccgcctt gacgtgggcc ggcatgtcgt
     1921 cgggcccttc cgtggtgtgg cgatagtaag gagcatcttc ggggacgagc tcgttgaagc
     1981 ttcgctccag gtcggtgcgc acggtcgcgt ccgcgttttc gttgatgcac aacgacgccg
     2041 aggtgtgctg gatgaagaaa tgcgccacgc cgacgcgaaa gcgcgcgagc tcggggatcg
     2101 cgcgcaccac ctcgtcggtg acgagatgga agccccgcgc tctcggcgcg agcacgatct
     2161 ctttctggag ccattccatg gcggcagggt agcacgatgg gcgccgcgcg ccgcaatgtt
     2221 ataatgaccg cgatgac
//
LOCUS       DASPGK010000147         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_390912, whole genome shotgun sequence.
ACCESSION   DASPGK010000147 DASPGK010000000
VERSION     DASPGK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390912"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VEC56_04265"
     CDS             <1..1329
                     /locus_tag="VEC56_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HXV13399.1"
                     /translation="PVPAPDQPAATEAPKAAPTPPVPVASTANDLDYSNFFWGSGKDP
                     FDILGPFDDWFDEARPQGYYLFGQPMASAPRPRVDIVEQLHKERLAGLINLASYNYLG
                     LSYRPDVIEAACDAIRRFGTGSSGSPILSGTTSLHDELAQTVARFKNKEECLLFPSGY
                     SANVGIIAGLMRSGDLVVTDQFAHASIVDGIILSKAQVRYFRHNNAADLERKLKDFPG
                     RKLVIVEGVYSMDGDLANLPEIVEVAKSNGARIMIDEAHSAFLFGPNGRGVAEHFGLD
                     DEIDIHFGTFSKSLGGQGGYVVGSRKLIRYLKGFGRSYVFSCALAPGVVGGLIKACQI
                     ARDEPQLRERLWANTAYLQEQLRERGVDIGDSQSQVIPIMIRDDDRVFKVAEDMIHNG
                     VYLNPVRYPAVGKHRSRLRISVTAAHELPELREAADIIARVLDRHGLLCR"
     gene            1320..1982
                     /locus_tag="VEC56_04270"
     CDS             1320..1982
                     /locus_tag="VEC56_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HXV13400.1"
                     /translation="MPLTRYHFKNAVNGFFEMSREHAKRLVPAHLEPMELKHSIGVFA
                     VTAFDFTKSEVGPYTELVMAVITPPRIGAGGEFPRSAFYPFVVGTSTEASRLHAIERW
                     HLPHYMADIAVDFHEDNGTMRVHAHEKGRPILDMTVSAHKWVPVNHLYQAFTVDSPRR
                     FKADIHMEGAFTEHEEETGELVLHPHPMCEAVLNADVASFPFRELWMRDGVQTFEELQ
                     TI"
BASE COUNT          366 a          733 c          648 g          358 t
ORIGIN      
        1 cccgttcccg cgccggacca gccggccgcg accgaggctc cgaaggccgc cccgaccccg
       61 ccggttccgg tggcgtcgac ggcgaacgac ctcgactaca gcaatttttt ctggggatcc
      121 ggcaaggacc ccttcgacat cctcgggcct ttcgacgact ggttcgacga agcccgaccg
      181 cagggctact acctgttcgg ccagcccatg gcgagcgccc cgcggccacg cgtcgacatc
      241 gtcgagcagc tgcacaaaga gcgcctggcc gggctcatca acctggcgtc gtacaactat
      301 ctcggtctct cctaccggcc ggatgtcatc gaagccgcgt gcgatgcgat tcgtcgcttc
      361 ggtaccggct cgagcggttc tcccatcctg agtgggacga cgtcgctcca cgacgagctc
      421 gcgcaaaccg tggcgcgctt caagaacaag gaggagtgcc tcctcttccc ctccggctat
      481 agtgccaacg tcggcatcat cgccggcctg atgcgctcgg gcgacctcgt ggtgaccgac
      541 cagttcgcgc acgcgagcat cgtcgacggc atcattctct cgaaggcgca agtgcgctac
      601 ttccgtcaca acaacgcagc cgacctggag cgcaagctca aggacttccc gggccgcaag
      661 ctggtcatcg tcgaaggcgt gtactccatg gacggcgatc tggcgaacct tcccgagatc
      721 gtcgaagtgg cgaagagcaa cggcgcccgc atcatgatcg acgaggcgca ctcggcgttt
      781 ctcttcggcc ccaacgggcg tggtgtggcc gagcacttcg gcctcgacga cgagatcgac
      841 attcacttcg gcacgttttc gaagagcttg ggcggccagg gcggctacgt ggtcggatcg
      901 cgcaagttga ttcgctacct caagggcttc gggcgctcgt acgtgttctc gtgtgcgctc
      961 gctcccggcg tggtcggcgg actgatcaag gcctgccaga tcgcccgcga cgagccccag
     1021 ctgcgcgagc gcctgtgggc caacacggcc tacctccagg agcagctgcg cgagcgcggc
     1081 gtcgacatcg gcgactcgca gtcgcaggtg atccccatca tgattcgcga cgacgatcgc
     1141 gtgttcaaag tggccgagga catgattcac aacggcgtct acctgaaccc ggtccgctac
     1201 cccgcggtcg gcaagcaccg ctcgcggctg cgcatctcgg tgacggccgc gcacgagctg
     1261 cccgagctgc gcgaggcggc cgacatcatc gcgcgcgtcc tcgaccgcca cggtctctta
     1321 tgccgctaac gcgctaccac ttcaagaacg cggtcaacgg attcttcgag atgtcgcgcg
     1381 agcacgcgaa gcgcctcgtg ccggcccacc tcgagccgat ggagctcaag cacagcatcg
     1441 gcgtgttcgc ggtaaccgcc ttcgacttca cgaagagcga ggtcggcccg tacaccgaac
     1501 tggtgatggc ggtcatcacg cccccgcgca ttggcgccgg cggcgagttc ccgcgctcgg
     1561 ccttctatcc cttcgtggtc ggaaccagta ccgaggcctc gcgcctgcac gcgatcgagc
     1621 gctggcacct cccgcactat atggccgaca tcgcggtcga ctttcacgag gacaacggca
     1681 ccatgcgcgt ccacgcgcac gagaaggggc gcccgatcct cgacatgacg gtcagcgcgc
     1741 acaagtgggt gccggtcaac catctctacc aggcgttcac ggtggactca ccgcgccgct
     1801 tcaaggccga catccacatg gaaggggcct tcacggagca cgaagaggag acgggcgagc
     1861 tcgtcttgca tccccatccg atgtgcgaag cggtcctcaa cgccgacgtc gcatcgttcc
     1921 cgttccgcga gttgtggatg cgcgacggtg tgcagacgtt cgaagaactc cagacgatct
     1981 agcggtcacg atcatgggtg agcaccgggg gcttccctcg gtgctcgtct cggcgggccc
     2041 agcccagcgg taatccatgt cgacgaatcg ctccatcttc gtgctcgtca atcccgccgc
     2101 gggac
//
LOCUS       DASPGK010000148         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1562302, whole genome shotgun sequence.
ACCESSION   DASPGK010000148 DASPGK010000000
VERSION     DASPGK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562302"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VEC56_04275"
     CDS             complement(<1..776)
                     /locus_tag="VEC56_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV13401.1"
                     /translation="MIMDNKSNGHAVGYPTDAIIALTYRCDARCEMCNIWQLKPQEFL
                     SVDDYAKVPSSLKDINVSGGEAFMRKDVVDIVKVIHQKCGDPRIVVSTNGFRTEQIIA
                     AMEELRKTIPNIGIGVSLDGIGETHNRIRGVKYAWENANATLRQLKERGFTNVRIGFT
                     AMNENVHEMRQVYDMACEMGIQFTTAVAQNSEIYFSTQVNRDVADAHLYDALGYVMKH
                     ELLSYHPKKWLRAYFENGTLIFNKEKRRILACRAGIDFFYL"
     gene            complement(773..1903)
                     /locus_tag="VEC56_04280"
     CDS             complement(773..1903)
                     /locus_tag="VEC56_04280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13402.1"
                     /translation="MIRERTRVAVVASTFGVGGSERVTADVLRRLDRDRFEVELYFLR
                     DAGVVGRELFREGFGGSERLGKRRRDRRALLRLLAHFRQFRPHVVFCLDHHDAMTAGR
                     LAGLAAGARGLVVASHATALIGRRRLFGLTDRLLMEFTRRVIAVSKTHAHHLRAQEGI
                     ERERIAVIENGIDPSAWPMVSPASRRAARAELGLEAAAHVVLMVAGMRPEKGHEVLLE
                     AVARAKDAGRSVRVLLAGDGERRGALEALALSLGIRGDVEFLGVRRDVARLLHASDVV
                     VLPSVVEALPMALLEAMAAGTPVIASAVGSVPELVQDGRTGLLIPPCDAASLADRIGV
                     ILDDAAGAARMRDGARECVVGRYSIDETAARYGDLFEDVVAA"
     gene            complement(1900..2946)
                     /locus_tag="VEC56_04285"
     CDS             complement(1900..2946)
                     /locus_tag="VEC56_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV13403.1"
                     /translation="MVRIGVIGCGYWGPNLIRNFSHSKGSQVLVCADLSEDRLTHMRT
                     LYPQIETTTDYRRVLARKDVDAVVVATPPETHVRLTVEALRAGKHVFVEKPLALSSAE
                     GTQMVEEALQAKRVLMVGHTFVYTAAVNKIKEVIESGELGEIFYISTTRVNLGIFQES
                     INVIWDLAPHDVSILSYILDAAPESVATQASCNIRKNVEDVAFLTLRYPGDVLAHVHV
                     SWLNPNKIRSTTVVGSKKMLVYDDVSALEKIRIYDKGVTVTPHYDTFGEFQLSYRYGD
                     ILIPRLDDAEPLKVACQHFIDCIEKGTVPRSSGVHGLNVVRVLEAAVESIREDGRMIS
                     VEPIGSGLAGTDET"
     gene            complement(2977..3720)
                     /locus_tag="VEC56_04290"
     CDS             complement(2977..3720)
                     /locus_tag="VEC56_04290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXV13404.1"
                     /translation="MKKNLATFRYTDHKVIPLYASEGARGAFPLAPASAASRYSSLAA
                     KRVVDIVFSLAVMVLGLPFYALIAALIKITSEGPVLFVQDRVGRDGQPFKFYKFRTML
                     VDCTDAQHRNFAEDFIKGRLMHDEENGRVFKLKNDPRVTAIGRFLRRTSLDELPQFIN
                     VLRGEMTLVGPRPPLAYELAHYKEWHRGRLAVKPGLTGLWQVSGRSTVPFDEMVMLDL
                     YYIENWSLALDMKIILRTVPVMVFGLGGY"
     gene            complement(3761..4879)
                     /locus_tag="VEC56_04295"
     CDS             complement(3761..4879)
                     /locus_tag="VEC56_04295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV13405.1"
                     /translation="MGLKQTVPFLDLTLQHRALRTEISAAVERTLDRGDFILGAPLEE
                     FEREFARYCDCEFAVGLSSGTAALHLALVALGVGAGDEVVTVPNSFIATVESILYTGA
                     TPVLVDVDPDTFVLDPAAFERAITPRTKAVVPVHLYGQPCDMETINAIASRHGIAVVE
                     DACQAHGATFHGRKTGSFGTAAAFSFYPTKNLGTIGDGGALTTNDRELAATVKALRHH
                     GQFEPNVFPRVGFNYRLDTMKAAVLGVKLAHLDAWNARRREIAQRYRARLKGTEFVFQ
                     ERVAGTEPVFHILAAKHPRQRVVQEALNAAGVGWGKHIVAPAHRHSGYVDLVRTAGSL
                     GVSEALSTELVSLPVFPELTDEQVDYAAEVLSKVEVSV"
     gene            complement(5004..>5903)
                     /locus_tag="VEC56_04300"
     CDS             complement(5004..>5903)
                     /locus_tag="VEC56_04300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13406.1"
                     /translation="AARSLAREPFDLVILEYWQMYPLLGIFPRERTVLLTHDIDVQVH
                     EARSQLERSVPARIRAATTFRAEAREEERAYRRAARVWALTRRDADAVRALTGREAAV
                     LPLGLAEEAFVAQPAPRSSREVLLMGAMHSAFNRDAIDHFVRDIHPALATLPGIRFTV
                     VGGALPPEVATFASVPGVEVTGHARDINPYLERAACMVVPLRFAGGIRIRILHAMARG
                     LPVVCSPVAVEGMGLEAGRHVLVAESPSEYRAHVQRVFDEPAFALDLARAARERVWSA
                     HGPDARGPGLRAFVERAMGERPS"
BASE COUNT         1020 a         1964 c         1920 g          999 t
ORIGIN      
        1 agataaaaga agtcgatgcc cgctcggcac gccaggatgc ggcgcttctc tttgttgaag
       61 atcaaggtgc cgttctcgaa ataagcgcgc agccacttct tcgggtggta gctcaagagc
      121 tcgtgtttca tcacatagcc gagtgcgtcg tagaggtgcg cgtcggcgac gtcgcggttg
      181 acctgggtcg agaagtagat ctccgagttc tgtgccaccg cggtggtgaa ctgaatcccc
      241 atttcgcacg ccatgtcgta cacctggcgc atctcgtgga cgttctcgtt catggcggtg
      301 aagccgatgc gcacgttcgt gaacccgcgt tccttgagct ggcggagcgt cgcgttcgcg
      361 ttctcccacg cgtacttcac gccccggatg cgattgtggg tctcgccgat cccgtcgaga
      421 gagacgccga tgccgatgtt gggaatcgtc ttccgcagct cttccatggc ggcgatgatc
      481 tgctcggtgc gaaagccatt ggtcgagacg acgatgcgag ggtcgccgca cttctggtgg
      541 atgaccttca cgatatcgac gacgtctttg cgcataaagg cttcgccgcc ggagacgttg
      601 atgtccttga gcgacgaggg caccttggcg tagtcgtcga ccgagaggaa ctcctggggc
      661 ttgagctgcc agatgttgca catctcgcag cgcgcgtcgc agcggtaggt gagcgcaatg
      721 atggcgtcgg tcgggtagcc gaccgcgtgc ccgttgctct tgttgtccat gatcatgccg
      781 ccaccacgtc ctcgaagaga tccccgtagc gcgccgccgt ctcgtcgatc gagtaacgac
      841 ccaccacgca ctcccgcgcc ccgtcccgca tccgcgccgc acccgcggcg tcatccagta
      901 taaccccgat gcggtcggcg agcgaagcgg cgtcgcaagg cggtatcagg aggccggttc
      961 ggccgtcctg gaccagctcg ggcaccgagc cgaccgcact cgcgatcacc ggcgtcccgg
     1021 ccgccatcgc ttccaggagc gccatcggga gcgcctccac caccgagggc agcaccacca
     1081 cgtcgctcgc atgcagcaac cgcgccacgt cgcgacgcac cccgaggaac tccacgtccc
     1141 cgcgaatgcc cagggatagc gcgagggcct cgagggctcc gcggcgctcc ccgtcgcccg
     1201 ccaggagcac gcgcaccgag cgccccgcgt ccttggcgcg cgccacggct tcgagcaaga
     1261 cctcgtgccc cttctccggg cgcatcccgg ccaccatgag aacgacgtgc gcggccgcct
     1321 ccagccccag ctcggcgcgg gccgcgcgcc ggctcgccgg cgagaccatc ggccacgccg
     1381 aggggtcgat tccgttctcg atcaccgcga tccgctcgcg ctcgatcccc tcttgcgcgc
     1441 gcaggtggtg cgcgtgggtc ttcgaaaccg cgatcacgcg gcgggtgaat tccatcagca
     1501 aacggtcggt gaggccgaac agccgtcggc gcccgataag ggcggtcgcg tgcgaagcga
     1561 ccacgagacc gcgggctccg gcggccagac cggccaagcg accggccgtc atcgcgtcgt
     1621 ggtggtcgag gcagaagacc acgtggggcc ggaactgccg gaaatgcgcg agcagccgaa
     1681 gcagtgcgcg ccggtcgcgg cggcgtttgc ccaagcgctc gcttcctccg aatccctcgc
     1741 ggaacaactc gcggccgacg actccggcgt cacgcaggaa atacaactcg acctcgaagc
     1801 ggtcgcgatc gagtcgccgg aggacgtcgg cggtgacgcg ctcggagccc ccgacgccga
     1861 aggtggacgc caccaccgcg acccgggttc gctcccggat cacgtctcgt ccgttccggc
     1921 caggccgctc ccgatcggtt cgacgctgat catgcgtccg tcttcgcgga tggactccac
     1981 cgccgcttcc aacacgcgca ccacgttcaa gccgtgcacg ccggagctgc ggggtacggt
     2041 gcccttctct atgcagtcga tgaagtgctg gcaggccacc ttgagcggtt cggcgtcgtc
     2101 caggcgcggg atcaggatgt cgccatagcg atacgagagc tggaactcgc cgaaggtatc
     2161 gtagtggggg gtgaccgtga cccccttgtc gtagatgcga attttctcga gcgcgctcac
     2221 gtcgtcgtac acgagcatct tcttgctgcc cacgaccgtg gtcgatcgga tcttgttggg
     2281 gttgagccag ctcacgtgga catgcgccag cacgtcgccc gggtaccgca aggtcagaaa
     2341 ggccacgtcc tcgacgttct tgcgaatgtt gcaactggcc tgggtggcca ccgactcggg
     2401 cgcggcgtcg aggatatagc tcaagatcga cacgtcgtgc ggggccagat cccagatcac
     2461 gttgatgctc tcttggaaga tcccgaggtt cacgcgcgtc gtgctgatat agaagatctc
     2521 gcccagttcg cccgattcga tcacttcctt gatcttgttg acggccgccg tatacacgaa
     2581 cgtgtggccc accatcagca cgcgcttggc ctgcagcgcc tcctcgacca tctgggtccc
     2641 ttcggcggac gaaagtgcca gcggcttttc gacgaatacg tgcttgccgg cgcgcagcgc
     2701 ttccacggtc aggcgcacgt gggtctccgg aggtgtggcc accacgaccg cgtcgacgtc
     2761 cttgcgagcg agcacgcgac ggtagtcagt cgtagtctcg atctgcgggt acagggtgcg
     2821 catgtgcgtg agccggtcct ccgagaggtc ggcgcaaacc agcacttgcg aacccttcga
     2881 gtgcgagaaa ttgcgaatga ggttggggcc ccaatagccg caaccgatga ctccgatgcg
     2941 aaccaaagca gctgtactcc tgtggatagg acggaatcaa tagccgccga gaccgaacac
     3001 catgacgggg acggtccgga ggatgatctt catgtcgagc gcgagcgacc agttctcgat
     3061 gtagtagagg tcgagcatca ccatctcgtc gaacggaacc gtgctgcgac cgctcacctg
     3121 ccaaagaccg gtgaggcccg gcttcaccgc caggcgcccg cgatgccact ctttgtagtg
     3181 cgcgagctcg taggccaagg gcggcctcgg gcccaccagc gtcatctcgc cgcgcagaac
     3241 attaataaac tgcggcagct cgtcgaggct ggtgcggcgc aggaaccggc cgatggcggt
     3301 tacgcgcggg tcgttcttga gcttgaacac gcggccgttc tcctcgtcgt gcatgaggcg
     3361 acccttgatg aagtcctccg cgaagttccg gtgctgcgcg tcggtgcagt cgaccagcat
     3421 ggtgcggaac ttgtagaact tgaacggttg cccgtccctg cccacgcggt cctgcacgaa
     3481 caacaccggg ccttcggagg tgatcttgat caaggcggcg atgagcgcgt agaacgggag
     3541 tcccagcacc atcaccgcga gcgagaacac gatgtcgacg acgcgcttgg cggccaggct
     3601 cgagtagcgg gaggccgccg aggcgggggc caggggaaat gcgccccttg ctccttccga
     3661 ggcgtacagg ggaatcacct tgtgatcggt atatctgaac gttgcaaggt tcttcttcac
     3721 ggctgctgac cttcttaaag ctggtgggtg caattcagat tcagaccgag acttcgactt
     3781 tggacagaac ctcggcggcg tagtcgactt gctcgtcggt cagttccggg aacaccggaa
     3841 gggacacgag ctccgtcgac aacgcctcgc ttaccccgag cgacccagcc gtgcggacca
     3901 ggtcgacgta accggagtgg cgatgcgcgg gtgccacgat gtgcttcccc caaccgacgc
     3961 cggccgcatt gagcgcttcc tgaaccacgc gctgccgggg gtgcttcgcc gccaagatgt
     4021 gaaagaccgg ttccgttccc gccacgcgct cctgaaatac gaactccgtg cccttcaaac
     4081 gcgcgcggta gcgttgcgcg atctcccgtc gcctcgcatt ccacgcgtcg agatgcgcga
     4141 gcttcacgcc caggaccgcc gccttcatcg tgtcgagccg gtagttgaaa ccgacccggg
     4201 ggaagacgtt gggctcgaac tggccgtggt gacgcagtgc cttcaccgtg gctgccagct
     4261 cgcggtcgtt ggttgtgagc gccccgccgt cgccgatcgt gccgaggttc ttcgtcggat
     4321 agaacgagaa cgcggccgcg gtcccgaacg aaccggtctt gcggccgtga aacgtcgccc
     4381 cgtgcgcctg gcaggcgtct tccacgaccg cgatcccgtg gcgggacgca atcgcgttga
     4441 tcgtctccat gtcgcagggc tgtccgtaca aatgaaccgg cacgaccgcc ttggtgcggg
     4501 gtgtgatcgc gcgttcgaag gccgcgggat cgagaacgaa cgtgtccggg tcgacgtcga
     4561 cgagcaccgg ggttgccccc gtgtagagaa tggactcgac ggtcgcgatg aacgaattgg
     4621 gtacggtgac gacttcgtcg cccgcgccca cgcccaacgc gaccagcgcg aggtgcaacg
     4681 ccgcggtacc cgagctcaag ccgaccgcga actcacagtc gcagtagcgc gcgaactcgc
     4741 gctcgaattc ctcgagcggg gcgccgagaa tgaagtcccc acggtcgagc gtgcgttcca
     4801 cggccgcgga gatctcagtt cgaagcgccc ggtgttgaag cgttaggtcc aggaagggaa
     4861 ccgtctgctt aagacccatc aatgacaccc ggatagttga cccttgcccc tgacgcgagt
     4921 cggttgattc tagcacagtt acgcggagcg aatccagccc caattttcca ccgttcgggg
     4981 gcctctagag ggactttggg gcgttacgac ggcctttccc ccatcgcgcg ctcgacgaac
     5041 gcccgcagcc caggaccgcg tgcgtcgggt ccgtgggcgc tccagacgcg ttctcgggct
     5101 gcgcgcgcca ggtcgagggc gaaggcaggc tcgtcgaaga cgcgctggac gtgggcccgg
     5161 tactccgacg gcgactcggc caccaggacg tgccgcccgg cctccaagcc catcccctcg
     5221 actgcaaccg gagagcacac gaccggcagc ccccgcgcca tcgcgtgcag gattcgaatc
     5281 cgaattcccc ccgcgaagcg cagcggtacc accatgcacg cggcgcgctc gaggtaggga
     5341 ttgatatcgc gcgcgtggcc ggtgacctcc acgcccggca cgctcgcgaa cgtggccacc
     5401 tcgggcggga gcgccccacc caccacggtg aagcgaatgc ccggcagggt tgccagcgcg
     5461 gggtgaatgt cgcgcacgaa gtggtcgatc gcatcgcggt tgaacgccga gtgcatcgct
     5521 cccatgagca gcacttcacg cgaggagcgc ggcgccggct gggcgacgaa ggcctcctcg
     5581 gccagtccca ggggaagcac cgcggcctcg cggccggtga gagcgcgtac cgcgtcggcg
     5641 tcgcggcgcg tgagcgccca cactctcgcg gcgcgccggt aggcgcgctc ctcttccctc
     5701 gcctccgccc taaacgtggt cgccgcgcgt atccgggccg gcacgctgcg ctcgagctgg
     5761 gaacgtgcct cgtggacctg aacatcgatg tcgtgggtga gcagcaccgt gcgctcgcgc
     5821 gggaaaatcc cgaggagcgg gtacatctgc cagtattcga ggatgaccaa gtcgaagggt
     5881 tcgcgcgcaa gcgatcgcgc ggc
//
LOCUS       DASPGK010000149         7882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_742664, whole genome shotgun sequence.
ACCESSION   DASPGK010000149 DASPGK010000000
VERSION     DASPGK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742664"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VEC56_04305"
     CDS             complement(<1..724)
                     /locus_tag="VEC56_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXV13407.1"
                     /translation="MYARTFRALAACFLVHALFVTAAPGQPLLDPVAHPKFVNSLPIA
                     ARIDLTAGGYWETSVTQFQQYLGLVDPMTGTPLATTVWGYHGSYPGPTLVARSGVPLD
                     IRWTNDLWGLPHLLPVDETLHWAMPEGYPESGVPIVTHVHGGHSESASDGLPDAWFTP
                     GFAQTGSGFVKETLHYDNDQEAGTIWYHDHALGITRLNVYAGLAGFYLIRDANEDALV
                     LANQLPTGPYEIEMAIQDRMFTA"
     gene            complement(877..952)
                     /locus_tag="VEC56_04310"
     tRNA            complement(877..952)
                     /locus_tag="VEC56_04310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Glu,seq:ttc)
     gene            1264..2631
                     /locus_tag="VEC56_04315"
     CDS             1264..2631
                     /locus_tag="VEC56_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13408.1"
                     /translation="MRIRIRAGVYLLSGSRVRAGKGDRTMNFNEPSLRAVASVVAFPM
                     LFQSALIAFAWGTGDATWLMVAKRIFLLLPACAVVAACWATVVSVLSIPIRSARHEFI
                     TALFVTWWDLGKSVLSFWGGLFAFAVKLPLAILALLRVTVIAVWALIADIVLMPFSFL
                     RHVGGNVVRSPIPWIAVSLTLAWCAIETTIFTYVMSPVVIDTFSNITGEALSETFVRV
                     PLFLFLFFVVLGSYAVLSTFVDALKSKSVQQIAGILVIECVVIFVEVMFLYREFVDSL
                     VPWFAQYSANFELGIYGTIGIACFAWFGIRSLSWFLFAAHGTPTIMSVIQGRGVEYTI
                     SGAAPRTRFFELSEALWTRTHQDSAWMRERGDSLLASFMLPPLQVVAAAVNFCTLCLL
                     NKHLFSLPFENLSAIRYSEKLIRDFKARSGFHGPSSAHPSGHAARRHDAEMDPDSMTI
                     TAGRS"
     gene            2673..3452
                     /locus_tag="VEC56_04320"
     CDS             2673..3452
                     /locus_tag="VEC56_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13409.1"
                     /translation="MTRSVAITASIVLVLGAAVLAGCEAAGGAESETPRQSLFIGVDV
                     SGSFMDSGHFNDSLEFLAYYIYGHLNGVGGMERPNTLFVSSIGGAREGEPKTFFPIQT
                     FENKSVEEIHATLCEMFPKENPNPFTDYNAFFEQVAFTVRSKNLVLRPITIVMVSDGV
                     PDVSRNGDTGYNSVVLHPLERLARSVTVRLLYTDATVGKSWQTEVSRKRVKVWTQDAD
                     VMRSWKDPKIMLADVPFGEQPHFFDWVEDNVDFGVRARRVD"
     gene            complement(3581..4210)
                     /gene="lexA"
                     /locus_tag="VEC56_04325"
     CDS             complement(3581..4210)
                     /gene="lexA"
                     /locus_tag="VEC56_04325"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXV13410.1"
                     /translation="MEKLPLTIRQKEILEYLGVHTRDYGYPPTVREICRATGLKSPRS
                     VSQHLQALERKGYIHRGRDKSRAIRFLHRPDSLGSNGSDHAVALPLKGHVAAGLAVNG
                     NEVDGASYYVDRGLFSGDGNFLMKVEGDCMVDAHILEGDLIVVSPEREAASGDVVVAR
                     IGGEVTVKRYEMRGGSEYLVSQMGPVKIDSKGPQVQILGRVVGLIRSAS"
     gene            complement(4167..7262)
                     /locus_tag="VEC56_04330"
     CDS             complement(4167..7262)
                     /locus_tag="VEC56_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXV13411.1"
                     /translation="MSTRNVRPFVHLRVLSSYSLGHGLSTPADLCRHARRVGFDAVAL
                     TDVGGTHGLVEFHRAAREVGVKPIYGTLLVLDWGVAPASRDPVQTLILLALDRVGLRH
                     VCAAATLSATRRERREPLTVADIEGCGDGVVAIAGFSAPADGPSPRHYLSALRGVFGD
                     RVFVEYRDGLSGEPMEMQARVVADAADVGVPAVLVQDVRFVGPARQQLIDLVASASER
                     AFEHRVFSDPRIGDSVPEHGMRTAAEMSAAYDAAPEAHSNAALIASLVQPDLLESLEV
                     HEGPAHADMFDPSGEASRVLRGRVEAAFEERFGRRDDAKVRRAVVQDELAALERAGLA
                     ETIVQFADVVERLRRAGVVVGPATGLSLQSACAYLLGLTTFDPYLIDAAFRPDFEDTS
                     ATRVLDVQTTPEFRPRVLGTLNRVFDGAGIGYVPTVEHITAARALRIVAKRLEDIPPE
                     VEDAIKVASRHHGVTLRELSEDNRSFGALYRKSAAFRDIVSHAAAIEGLPYGYARTKR
                     SVIVSPKPLRAAFAFTVDPETGDRFVQATRDSFPVGDIRRVDVATLQVLAVVDDAGAD
                     TTELDRDALVLVARGDLDGVYLLEGAPGRLAATFGISTFADLVHFVALLRQRRTGTPL
                     AERVATFRDEGRVVPAAKAVGDVLTSTYGVLLFEDQMRDVVARLTRQSAADASALAAR
                     FGDHAPGNLAALRREFFAYAVEAAVSFEDATAWFTRLLRHAHDVQNRQRVIAEALIVR
                     RCLAAKARDRAAFFARFLDAPLDADKRARYRALLEQEGTWLAPSITASERGHAVEGER
                     VRAPLWTIDGVTRDASDTIIRMRGAKKVTTAEEFRFAALNAGINLEVIDALIRAGAID
                     GAAGTGKEGTGRTRPVLSSVAASGQMNMVLSGTVPEGSTPPGPAFAQSPSAQKDGNSR
                     HGFRVVPSLSEFYPHPSATPVELAGRIRNFHDFKTSSGKTVGFFELFDSSGSVRVFVP
                     WERVARIGEPLSDGCRVTVKGKVRLRDGRKVCDALEIVVAEGGNGHGETSPDDPSKGD
                     P"
     gene            complement(7313..7389)
                     /locus_tag="VEC56_04335"
     tRNA            complement(7313..7389)
                     /locus_tag="VEC56_04335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7353..7355),aa:Lys,seq:ttt)
     gene            complement(7460..>7882)
                     /locus_tag="VEC56_04340"
     CDS             complement(7460..>7882)
                     /locus_tag="VEC56_04340"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HXV13412.1"
                     /translation="GHEYGTTTGRPRRCGWFDAVAARTVVKINGMTDIALTKLDVLDT
                     FASVKVCHAYRIGKRTLDVFPADARDVESVVPTYETRPGWKEATTGRRLEDLPRPAVD
                     YIRHLESLVSCPIHLVSLGPERSAMVRIPRGPASVLGA"
BASE COUNT         1335 a         2610 c         2583 g         1354 t
ORIGIN      
        1 cggccgtgaa catgcggtcc tggatcgcca tttcgatctc gtagggtccc gtgggcagct
       61 ggttcgcgag cacgagtgcg tcctcgttcg cgtcgcgaat cagatagaag cccgccaacc
      121 ccgcgtatac gttgaggcgc gtgattccca gggcgtggtc gtgataccag atcgtccccg
      181 cttcctggtc gttgtcgtag tgcagcgttt ccttcacaaa tccactgccg gtctgtgcga
      241 aaccgggcgt gaaccacgcg tccggaagac cgtcgctcgc cgattcactg tgtcccccgt
      301 ggacatgggt cacgatgggg acgccggatt cagggtaccc ctcgggcatg gcccagtgga
      361 gggtctcatc gaccggcaga aggtgtggga gcccccacag atcgttcgtc caccggatat
      421 ccaggggcac gccgctgcga gccaccagtg tcggccccgg gtagctgccg tgataccccc
      481 acaccgtcgt cgcgagcgga gtaccggtca tgggatcgac gagtccaagg tactgctgga
      541 actgcgtgac ggacgtctcc cagtagccgc cggcggtcaa gtcgatgcga gcggcaatgg
      601 ggagtgaatt gacgaacttg ggatgggcga ccggatcgag aagaggttgg cccggtgctg
      661 cggtgacaaa tagcgcgtgc acgaggaagc acgctgccag ggcccgaaat gtgcgggcgt
      721 acataaatag ccccctttcg ctgctcgacg cgagcagcgc agacgttcac tgcatacatg
      781 aatcgagccc gggggccccg cgactcgcct gccagtatac ataccctgca ccgaaataca
      841 agacaaaatg gtcttgtttc ccagagggcg agaaaatggc gtccccaacg ggatttgaac
      901 ccgtgtcgcc gccgtgaaag ggcggtgtcc tgggccacta gacgatgggg acgccgaacg
      961 gtgctggata gagttgtgga ggggcaaggc tacggccgtg ggggggcgct gtcaaacgaa
     1021 aacgcctgca accggtcgag gccggcgctc ggggttgcga cgccgaagcc ggagcgtcgc
     1081 aatttgacac gtcggcggcg gtgcctggcc gcgtgtggcc atccacgcac gcggcccatg
     1141 cgacacgctc ttccctcgca ctcccgcatg cgttgcgacc gtccgtcgag acacgatcac
     1201 tggcacgctg catgcggctg taatcccgct tctggaaact tcaccatcgt cacgcgccgg
     1261 cggatgcgaa ttcggatccg cgcgggcgtc tacttgctgt ccggctcgcg cgtgcgtgcc
     1321 ggcaaggggg atcgcacgat gaatttcaac gaaccctcgc ttcgcgccgt ggccagcgtc
     1381 gtggcctttc cgatgctgtt tcagagcgcg ctcatcgcct tcgcgtgggg cacgggggac
     1441 gcgacctggc tgatggtcgc caagcggata ttcctgctgc tccccgcgtg tgccgtcgtg
     1501 gccgcgtgtt gggccacggt cgtgtccgtg ctgtccattc ccatccggag cgcgcgccac
     1561 gagttcatca cggcgctgtt tgtcacctgg tgggacctcg gcaagtccgt gctctcgttc
     1621 tggggcggct tgttcgcctt cgcggtgaag ttgccgctgg cgatcctcgc gctccttcgc
     1681 gtcacggtga tcgcggtgtg ggcgctgatc gcggacatcg tgctgatgcc cttctcgttc
     1741 ctgcgccacg tgggggggaa cgtcgtgcgc tcgcccatcc cgtggatcgc ggtgtcgctg
     1801 acgctggcct ggtgtgcgat cgagaccacg atcttcacct atgtcatgtc tccggtggtc
     1861 atcgacacgt tctcgaacat caccggcgag gcgttgagcg agaccttcgt gcgcgtgccg
     1921 ctcttcttgt tcctattttt cgtcgtcctg ggcagctacg cggtcttgtc gaccttcgtc
     1981 gacgcgctca agagcaagag cgtccagcaa atcgccggga tcctggtgat cgagtgcgtc
     2041 gtgatattcg tcgaggtcat gttcctctac cgcgagttcg tcgactcgtt ggtgccctgg
     2101 ttcgcgcagt actcggccaa cttcgagctc ggcatctacg gcaccatcgg catcgcctgc
     2161 ttcgcctggt tcggtatccg cagcttgtcg tggttcctgt tcgccgccca tggcacgccg
     2221 accatcatgt cggtgatcca gggacgcggg gtcgaataca ccatttccgg cgcggcaccg
     2281 cgcacgcgct tcttcgagct gagcgaggcg ctgtggacgc gaacccacca ggacagcgcg
     2341 tggatgcgcg agcgaggcga ctcgttgctg gcgtcgttca tgctgccgcc gctccaggtg
     2401 gtggcggctg cggtcaactt ctgcaccctg tgcttgctca acaagcacct gttctcgctg
     2461 ccgttcgaga acctgagcgc gatccgctac tcggaaaagc tgattcgaga cttcaaggcg
     2521 cgctcgggct tccacggccc gtcgtccgct catcccagcg gccacgccgc cagacggcac
     2581 gacgccgaaa tggatcccga ttccatgacc atcacggcgg gcagatcgtg aaggggtcgc
     2641 gactaggggc ttcgtcccgc aggaggatca tcatgacacg gagcgtcgct atcaccgcct
     2701 cgatcgttct ggtactcggc gcggccgtcc tggccgggtg cgaggccgcc ggcggggcgg
     2761 agtccgaaac gcccaggcag tcgttgttca tcggcgtcga cgtgagcggg tcgttcatgg
     2821 acagcgggca cttcaacgac tcgctcgagt tcctcgccta ctacatctac ggacacctca
     2881 acggggtggg cgggatggag cggcccaaca cgctgttcgt gagttcgatc ggcggggcgc
     2941 gcgagggcga gcccaagacg ttcttcccca tccagacctt cgagaacaag tcggtggagg
     3001 agattcacgc caccctgtgc gagatgttcc ctaaggagaa ccccaatccg ttcacggact
     3061 acaacgcatt cttcgagcag gtcgcgttca ccgtacgcag caagaacctg gtgctgcgcc
     3121 cgattaccat cgtcatggtg agcgacggtg tacccgacgt gtcccgcaac ggcgacacag
     3181 gctacaactc cgtcgtgctg cacccgctgg agcggttggc gcgcagcgtc acggtacggc
     3241 tcttgtacac cgacgcgacc gtcggcaaga gctggcagac ggaagtgagc cgcaagcggg
     3301 tcaaggtgtg gacgcaggat gccgacgtca tgcgctcgtg gaaggatccc aagatcatgc
     3361 tggcggacgt tcccttcggc gagcagccgc acttcttcga ctgggtcgaa gacaacgtcg
     3421 acttcggcgt gagggcacga cgagtggact gagagcggtg aggcaagaat gatattccgc
     3481 ggcgaaggca gtcgccggcg gacggcgttc gcggcgcgcg ggtaggggtg cgcgccgcgt
     3541 ccacgcgttc ggggctagta ctggggcgag acgttttacc tcaagacgcc gatcggatca
     3601 atccgaccac gcgtccgaga atctgtacct gcggtccttt cgagtcgatc ttgaccggtc
     3661 ccatctgcga cacgaggtac tcgcttccac cgcgcatctc gtaacgcttg accgtgacct
     3721 ctccgccgat ccgggcgacc acgacgtctc cgctcgcggc ctcccgctcc ggcgatacca
     3781 cgatcagatc gccttccaag atgtgcgcgt cgaccatgca atcgccttcc accttcatga
     3841 ggaagttgcc gtccccggaa aacaacccgc ggtcgacgta gtaactcgct ccgtcgacct
     3901 cgttaccgtt gacggccagg ccggccgcga cgtgcccctt gagcggcagc gccaccgcgt
     3961 gatccgaccc gttcgaaccc agagaatcgg gacgatgcag gaaacgaatc gcgcgcgact
     4021 tgtcccgccc ccggtggatg tagcccttac gctcgagcgc ctgcagatgc tggctcaccg
     4081 agcgcggtga cttcagcccc gtggcacggc agatctctct gaccgtcggc gggtaaccgt
     4141 aatcgcgcgt gtgcacacct agatactcaa ggatctcctt ttgacggatc gtcaggggaa
     4201 gtttctccat gaccattccc tccttcagcg actacgatct ccagcgcatc gcacaccttt
     4261 cttccatcgc gcaaccgaac cttgcccttt acagttacgc gacacccgtc gctcaatggc
     4321 tcgccaattc gagcgacgcg ctcccagggt acgaagacgc gcacggaacc actggagtcg
     4381 aatagttcga agaaaccaac cgttttccct gaactggtct tgaagtcgtg gaagttccgg
     4441 atgcgcccgg ccagttcgac tggggtggcg ctcgggtggg gatagaactc cgacaacgat
     4501 gggactacgc gaaaaccgtg ccgcgagttg ccatcttttt gtgccgatgg cgattgggcg
     4561 aaggcgggac caggtggagt ggagccttcg ggcacggtac ccgagaggac catattcatc
     4621 tgtccactgg ccgcaacgga cgacaaaacg ggccgggttc ggcccgtacc ctccttccct
     4681 gtccctgcgg caccatcgat tgcccctgcc cggatcagcg catcgatcac ctccaaatta
     4741 atgcccgcat tcaaagcggc aaagcggaat tcctcggcgg tcgttacctt cttcgcgccc
     4801 ctcatacgga tgatggtgtc cgacgcatcg cgtgtgacac cgtcgatcgt ccacaacggc
     4861 gcacgcacgc gttccccttc aaccgcgtgt ccgcgctcgc tcgcggtgat gctgggcgcg
     4921 agccaggtcc cctcttgctc gagtagcgcg cggtaacgcg cccgcttgtc ggcatcgagg
     4981 ggcgcgtcca aaaagcgcgc aaagaacgcg gcgcggtcgc gcgccttggc agccaggcac
     5041 cgccgcacga tcaatgcttc ggcgatcacg cgctgccgat tctgcacgtc gtgggcgtgg
     5101 cgcaggagtc gtgtgaacca cgcggtcgcg tcttcgaaag agaccgccgc ttccacggcg
     5161 taggcgaaga actcgcgccg caacgccgcc aggttccccg gcgcgtggtc gccaaaacgg
     5221 gccgccagag cggacgcatc ggccgcggac tgccgcgtca gccgtgccac cacgtcgcgc
     5281 atttggtcct cgaagagcag gacaccgtag gtcgaggtca gaacgtcgcc taccgccttc
     5341 gccgcgggca ccacacgacc ttcgtcgcgg aaggtcgcga cacgctccgc aagcggcgtc
     5401 ccggttcggc gctggcgcag caacgcgacg aagtgcacca ggtcggcgaa cgtcgatatg
     5461 ccgaaggtcg ccgccagccg acccggggca ccttcgagca ggtaaacgcc gtcgaggtcg
     5521 ccgcgtgcca ccagcaccaa ggcgtcgcga tcgagctcgg tcgtatctgc tcccgcgtcg
     5581 tccacgaccg cgaggacctg aagggtcgcg acgtcgacgc gccggatgtc accgaccggg
     5641 aacgaatcgc gcgttgcctg cacgaagcgg tcgcccgttt cggggtcgac ggtgaaggcg
     5701 aaggcggccc gcagtggctt gggcgagacg atgacactgc gcttggttcg cgcgtagccg
     5761 tacggcagcc cttcgatcgc ggccgcgtgc gacacgatgt ctcggaacgc ggcgctcttt
     5821 cgatagagcg ccccgaacga acggttgtcc tccgagagct cgcgcaaggt gacgccgtgg
     5881 tgacgcgacg ccactttgat ggcgtcctcg acctccggtg gaatgtcttc caagcgcttc
     5941 gctacgatcc gcagcgcccg tgccgccgtg atgtgctcga ccgtgggcac gtacccgatg
     6001 ccggccccgt cgaacacgcg attcagcgtg cccaggacgc gcggccgaaa ctcgggggtg
     6061 gtctggacgt cgagtacgcg cgtcgcgctc gtgtcctcga agtcgggacg aaacgcggcg
     6121 tcgatcaaat aggggtcgaa tgtcgtcagg cccaagaggt acgcgcacgc cgactgcagc
     6181 gagagtccgg tcgccggccc caccaccaca cccgcgcggc gcagccgttc caccacgtcg
     6241 gcgaactgca cgatcgtctc cgccagcccc gcgcgctcga gggcggcgag ctcgtcctgc
     6301 acgaccgctc ggcgaacctt ggcgtcgtcg cggcgcccga atcgttcttc gaaggccgcc
     6361 tcgacgcgcc cgcgcagcac gcgcgacgcc tcgcccgagg ggtcgaacat gtcggcgtgc
     6421 gcggggccct cgtggacttc gaggctctcc aggagatcgg gctgcaccag cgacgcgatc
     6481 agcgccgcgt tggaatgcgc ctcgggcgcg gcgtcgtacg ccgccgacat ctcggccgcc
     6541 gtgcgcatgc cgtgttcggg aaccgaatcg ccgatgcgcg ggtcgctgaa cacccggtgc
     6601 tcgaaggcgc gctcgctggc actggcgacg aggtcgatga gctgctggcg ggccgggccg
     6661 acgaagcgca cgtcctggac cagcacggcc ggcacgccga cgtcggcggc gtccgcgacc
     6721 acgcgcgctt gcatctccat cggctccccc gacaggccgt cgcggtactc cacgaacacg
     6781 cggtcgccga acacgccgcg cagcgcggat aagtagtggc gcggcgaggg tccgtcggcc
     6841 ggcgccgaaa acccggcgat cgcgaccacg ccgtcgccgc acccttcgat atccgcgacc
     6901 gtaagcggct cacgccgctc gcgacgcgtg gcggagaggg tggccgccgc gcacacgtgg
     6961 cgaagcccga cgcggtcgag ggccagaagg atgagcgtct ggacgggatc gcgagacgcg
     7021 ggcgcgacac cccaatcgag caccagcagc gtgccgtaga tcggcttgac gccgacctcg
     7081 cgcgccgcgc gatgaaactc gaccagaccg tgggtgccgc cgacgtcggt cagggcgacc
     7141 gcgtcgaaac cgacgcgccg tgcgtggcgg cacaggtcgg cgggtgtcga gaggccgtga
     7201 cccaggctgt aggaggatag cacccgcagg tggacgaacg gtctgacgtt gcgggtcgac
     7261 atggtctcgt ccccgttcca gccgtgctcg gccgacgcgg aggagaaaaa ggtggtgagc
     7321 cgcccaggac tcgaacctgg caccacctgc ttaaaaggca ggtgctctaa cctgatgagc
     7381 tagcggctcg acgtgaggcc gtcactttac gaatgccctc gtccgatgtc aaggacgggc
     7441 gccggtatgc ggcaagcgac tatgcgccca gcaccgacgc ggggccgcgc gggatgcgta
     7501 ccatcgcgga gcgctcggga ccgagcgaga cgaggtggat cgggcacgac accaggctct
     7561 ccagatggcg aatatagtcg accgccggcc gcggcaggtc ctcgaggcgg cgtccggtgg
     7621 tcgcctcctt ccaaccgggg cgcgtctcgt acgtgggcac cacggactcg acgtcgcgcg
     7681 cgtcggccgg gaacacgtcc agcgtgcgct tgccgatgcg ataggcgtgg cacaccttga
     7741 ccgaggcgaa ggtgtccagt acgtcgagct tggtgagcgc gatgtcggtc atcccgttga
     7801 tcttcacgac cgtgcgcgcg gccacggcgt cgaaccagcc gcaccggcgc gggcgcccgg
     7861 tcgtcgtgcc gtactcgtgc cc
//
LOCUS       DASPGK010000150         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_742851, whole genome shotgun sequence.
ACCESSION   DASPGK010000150 DASPGK010000000
VERSION     DASPGK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742851"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..431)
                     /locus_tag="VEC56_04345"
     CDS             complement(234..431)
                     /locus_tag="VEC56_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004043415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 domain-containing protein"
                     /protein_id="HXV13413.1"
                     /translation="MAKAAKKAASSDLIISKSRTKAAAKGYNVSSDFYGALDKAVRVI
                     IKNAGDRAKANKRKTLKPGDL"
     gene            606..1658
                     /locus_tag="VEC56_04350"
     CDS             606..1658
                     /locus_tag="VEC56_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13414.1"
                     /translation="MIIRPHMTASSPSAIPRIEFSDDDVTFFTIGDDAFFPGVAGLIN
                     SLRLLGYAQRIVIADCGFTPPQRELLSPHATLIPLSRAEVKNPQQYKAFPHLLRPRGT
                     VAIVDSDIIVTDSIDDMIASARAGKICVFPDPEEHRWFAEWEGVFALAAPPRKQTYVN
                     SGFEVFSTTHWPALLGRWWQACERIFPQPTIREGAARDDPTSQSDQDALNALLMSEFP
                     ADAIAPQAKNREVFRWDFERVRVRDAATLHCEFEGTRVAALHAIAGPKPWQRAAWPEA
                     TRRNAYLRVFRRLLAGDDVAVRVPPGELPPWLRRGVVGRLSLEGLGAMRAARARLPRD
                     TGVVTRARRLARRPKG"
     gene            complement(1662..2219)
                     /gene="dcd"
                     /locus_tag="VEC56_04355"
     CDS             complement(1662..2219)
                     /gene="dcd"
                     /locus_tag="VEC56_04355"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HXV13415.1"
                     /translation="MSIKPDKWITRMVKEYKMIEPYVESQVKDNAVSFGVSSYGYDIR
                     IADEFKIFTNINTTVVDPKNFDPKSFVDFKGEVAIIPPNSFALGRSVEYFRIPRNVLT
                     ICVGKSTYARCGIITNVTPFEPEWEGFVTLEISNTTPLPAKIYANEGIAQVLFFESDE
                     DCLVSYKDKKGKYQKQQGITLPRVP"
     gene            complement(2261..>2843)
                     /gene="folE"
                     /locus_tag="VEC56_04360"
     CDS             complement(2261..>2843)
                     /gene="folE"
                     /locus_tag="VEC56_04360"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXV13416.1"
                     /translation="PPSRKKRPPADALSHSVRVFLSALGRDPKAPELRETPDRVAEAW
                     RHFTRGYQSTAAEIFSEGVFEDSYEGMVLVRDIDFFSVCEHHLVPFFGKCHVAYVPAR
                     RVAGLSKVARLVELHARRLQVQERMTREIARDLAQALKPRGVAVRVEARHLCMAMRGV
                     EQKNADVVTVYATGVMKTDPALAEEFRAALTRR"
BASE COUNT          462 a          938 c          922 g          521 t
ORIGIN      
        1 caccacctac tactgggcgg tgtccctggc ttgcggcata gacatcggtg ttcgcagccc
       61 ggtatggacg ttcacgacgg gtgcgggacc ggtgcccgtg aaatcctcga cgtggggtgc
      121 agtgaaggcg ctgtaccggc cctagccgct cgccgagaga aaacacgaag gcccggccga
      181 cgaggccggg ccttccacga gtacctttcg gtacgaagga accaaactcc gatttacaga
      241 tcgccgggct tgagcgtctt gcgcttgttc gccttggcgc ggtcgcccgc gttcttgatg
      301 atgacgcgga cggccttgtc gagcgcgccg tagaagtcgc tcgatacgtt gtaacccttc
      361 gccgccgcct tggtacgcga cttcgaaatg atcaggtcgc tcgacgcggc cttcttcgca
      421 gcttttgcca tttcggcttc cctccaggag gaatgtgaac gtcttcgaaa aatcggctca
      481 ccagagcctc gttgtcggtc tcggaatacc aggaacgccg gagcctgtca aacacaaaat
      541 aggcctaaac ggccgaagtc gggcatcccg ggccgatcgg cggttgacac tgcttcccgg
      601 gagtcatgat aatccgacca cacatgaccg cctcgagccc gtccgcgata ccgcgcatcg
      661 aattctccga cgacgacgtc accttcttca cgatcggtga cgacgcgttc ttcccgggcg
      721 ttgcgggcct gatcaactcc ttgcgtttat tggggtacgc gcagcgcatc gtgatcgcag
      781 actgcggctt cacgccgccg cagcgggagc ttctttcgcc ccacgccacg ctgatcccgc
      841 tctcgcgcgc cgaggtcaag aacccgcagc agtacaaggc gttcccccac ctcctgcgcc
      901 ctcgcgggac ggtggccatc gtcgactccg acatcatcgt caccgattcg atcgacgaca
      961 tgattgcgtc ggcgcgcgcg gggaaaatct gcgtgtttcc cgaccccgag gagcaccgtt
     1021 ggttcgcgga gtgggagggc gtgttcgcgc tcgcggcccc gccgcgaaag cagacctatg
     1081 tcaactcggg cttcgaggtg ttctcgacca cgcactggcc ggcgctcttg ggtcgctggt
     1141 ggcaggcgtg cgagcgcatc ttcccgcagc cgaccatccg cgagggcgcc gcgcgcgacg
     1201 accccaccag ccaatcggac caggatgcac tcaacgcgct cttgatgagc gagttccccg
     1261 ccgacgcgat cgcgccgcaa gccaaaaacc gcgaggtctt tcggtgggac ttcgagcggg
     1321 tgcgcgtgcg cgacgcggcc acgctgcact gcgagttcga gggcacgcgc gtcgccgcgc
     1381 tgcacgcgat cgcggggccg aagccgtggc agcgcgcggc ctggccggag gcgacgcggc
     1441 gcaacgcata cctgcgcgtg ttccggcgtc tgctcgccgg cgacgacgtg gcggtgcggg
     1501 taccgccggg cgaactgccg ccgtggctgc ggcgcggtgt cgtagggcga ttgtcgctcg
     1561 aaggactcgg cgcgatgcgc gcggcacgcg cgcggctccc gcgcgacacc ggggtggtca
     1621 cgcgtgcgcg ccggctcgcg cgacgcccga aggggtagcg cctacggcac cctcggtagt
     1681 gttatgccct gctgcttctg gtacttgccc ttcttgtcct tgtaggacac caggcagtcc
     1741 tcgtcgctct cgaagaacag tacctgcgcg atgccttcgt tggcgtagat cttcgcgggc
     1801 agcggtgtgg tgttcgagat ctccagcgtc acgaagcctt cccactcggg ctcgaacggc
     1861 gtcacgttgg tgatgatgcc gcagcgcgcg taggtcgact tgccgacgca gatcgtcagc
     1921 acgttgcgcg ggatgcggaa gtactccacg cttcgcccca gcgcgaacga gttgggcggg
     1981 atgatggcga cctcgccctt gaagtcgacg aacgacttgg ggtcgaagtt cttgggatcg
     2041 acgacggtgg tattgatatt ggtgaagatc ttgaactcgt ccgcgatgcg aatgtcgtag
     2101 ccgtacgacg agaccccgaa ggacaccgcg ttgtccttga cctgcgattc cacgtagggc
     2161 tcgatcatct tgtattcctt caccatgcgc gtgatccact tatcgggctt gatcgacatg
     2221 cctgtctcct attctatcct gactcttgcc tagtttccgt tcaacgccgg gtgagcgcgg
     2281 cgcgaaattc ctcggccagc gcggggtcgg tcttcatgac gccggtcgcg tacacggtga
     2341 cgacgtcggc gttcttctgc tcgacgccgc gcatcgccat gcacaagtgg cgcgcttcca
     2401 cgcgcaccgc aacgccgcgg ggcttgagcg cctgcgccaa gtcgcgcgcg atctcgcgcg
     2461 tcatgcgctc ctgcacctgc aagcggcgcg cgtggagttc gaccaggcgc gccaccttgc
     2521 tcaaccccgc gacccggcgc gccggcacgt acgcgacgtg gcacttcccg aagaacggta
     2581 ccaagtggtg ctcgcacacc gagaagaagt cgatgtcgcg aaccagcacc attccctcgt
     2641 aggagtcctc gaacacgcct tcggagaaga tctcggccgc ggtcgattgg tagccgcgcg
     2701 tgaagtggcg ccacgcctcg gcgacgcggt cgggggtctc gcgcaactcg ggagccttgg
     2761 ggtcgcgccc caacgcggac agaaagaccc gcaccgagtg cgagagcgcg tcggccgggg
     2821 ggcgtttctt gcgcgatggg ggg
//
LOCUS       DASPGK010000151         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1327958, whole genome shotgun sequence.
ACCESSION   DASPGK010000151 DASPGK010000000
VERSION     DASPGK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327958"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="plsX"
                     /locus_tag="VEC56_04365"
     CDS             complement(<1..650)
                     /gene="plsX"
                     /locus_tag="VEC56_04365"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXV13417.1"
                     /translation="MRIAVDAMGGDKGPKVVVPGAIDAVLARRGEVRLILVGREEEIR
                     PLVGGVDPAMVEIVHASEVIDMHESPATAIRRKKDASITVALRMVREGKADAMVSAGN
                     TGAVVASSLLTIGRLHGVSRPAISTLWPNKRGGAVVLDVGANHECTPKNLVQFAIMGS
                     VFAEHHLGVARPRVGLLNIGEEKSKGNELVRDSYLLLEADPRVNFVGNVEGRDVFDG"
     gene            complement(661..846)
                     /gene="rpmF"
                     /locus_tag="VEC56_04370"
     CDS             complement(661..846)
                     /gene="rpmF"
                     /locus_tag="VEC56_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXV13418.1"
                     /translation="MGLPKRRHSKTRKRKRRTHWKLSTPSLTKCSHCGQPKTPHSVCP
                     HCGFYAGREVVRIEEEL"
     gene            complement(923..1402)
                     /locus_tag="VEC56_04375"
     CDS             complement(923..1402)
                     /locus_tag="VEC56_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXV13419.1"
                     /translation="MKVDLRELVETSGVLEGDESVAFLDPVGEEIVAPCHVRVEYRQS
                     QGTLHFSGAVATTLLTQCHRCLDPVRQDVAGEFDVMVRRGEHAAEAGDDVVALPLNQY
                     EIDLEPYVHEAVVLATPMLVLCREDCRGLCPSCGVNWNRETCSCRPSADSRWDALRK"
     gene            complement(1463..1864)
                     /gene="ndk"
                     /locus_tag="VEC56_04380"
     CDS             complement(1463..1864)
                     /gene="ndk"
                     /locus_tag="VEC56_04380"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXV13420.1"
                     /translation="MKTLLMIKPDAVAAGHVGDILWLVEKNRFAIRRLAKTQFSRESA
                     ERFYAVHRERPFFKDLVAYITSGPVVAIEIEADEAVSRIREFIGATNPADARPGTVRY
                     MFGKSLQNNAVHGSDAAENAQKELEIVFGGA"
     gene            complement(1892..2782)
                     /gene="sucD"
                     /locus_tag="VEC56_04385"
     CDS             complement(1892..2782)
                     /gene="sucD"
                     /locus_tag="VEC56_04385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXV13421.1"
                     /translation="MSMLIDRNTRLVVQGITGRDGSFHASQMAAYGTNLVAGVTPGKG
                     GTTFENTVPIFDSVHEAVDATGANTSVIYVRAPFAADAICEAADAGVRLVVCITEGVP
                     VRDMLRVVPFLKERATRLVGPNCPGLIAPNARVKVGILPASIVHPGSVGVVSRSGTLT
                     YEVVYQLTRAGMGQSTCLGIGGDPVIGTNFIDALRLFQADEETEAIVLIGEIGGSDEE
                     EAAAFIKEKVTKPVVSFIAGQTAPPGKRMGHAGAIVTGGSGTAADKIAAFAAVGIPVA
                     KVPSEITSLVASAAPSLKRA"
BASE COUNT          476 a         1011 c          914 g          526 t
ORIGIN      
        1 ccatcaaaca cgtcacgccc ttctacgtta ccgacaaagt tcacgcgtgg gtcggcttcg
       61 agcaacaggt aggagtcgcg caccagctcg ttgcccttgc tcttctcttc gccgatgttc
      121 aagagtccga cgcgcgggcg cgcgacgccc aagtggtgct cggcgaacac cgagcccatg
      181 atcgcgaatt ggaccaagtt cttgggcgtg cactcgtggt tggcgcccac gtcgagcacc
      241 accgctcccc cgcgcttgtt gggccacagc gtggaaatgg ccgggcgcga gacgccgtgc
      301 aggcgtccga tcgtcaacag cgagctcgcc accaccgccc ccgtgttacc cgcgctcacc
      361 atcgcgtccg ccttcccttc ccgcaccatg cgcagcgcga ccgtgatgga cgcgtctttc
      421 ttgcgccgga tcgcggtcgc gggcgactcg tgcatatcga tgacctcgga ggcgtgcacg
      481 atttcgacca tggcggggtc gacgccaccc accagcgggc ggatctcttc ctcgcgcccc
      541 accagtatca ggcgcacctc tccgcgccgc gccagaaccg catcgatcgc gccgggaacg
      601 accaccttcg ggcccttgtc gcctcccatc gcgtcaacgg cgattcgcac gcatcacctc
      661 ttagagctct tcttcgatgc ggacgacttc ccgccccgcg tagaacccgc agtgcgggca
      721 cacgctgtgc ggcgtcttcg gctggccgca gtgcgagcac ttggtgagac tcggcgtcga
      781 cagcttccag tgcgtgcgac gtttgcgctt gcgcgtcttc gaatgccttc tcttgggcag
      841 acccatgact tctccttacc tcacgaacga ttacttcgac ttcacgcgcg gccgcgaaag
      901 caccggatgc gcgcacctac tcctatttac gaagcgcgtc ccagcgcgag tcggcactgg
      961 gccggcacga gcaggtttcg cggttccaat tgacgccaca cgacggacac aagccgcgac
     1021 aatcctcgcg gcacaggacg agcatcgggg tcgcgagtac caccgcctcg tgcacgtacg
     1081 gctcgagatc gatctcgtac tggttcagcg gcagcgccac cacgtcgtcg cccgcttcgg
     1141 ccgcgtgctc gccgcggcgt accatcacgt cgaactcgcc ggctacgtcc tgccggaccg
     1201 ggtcgagaca gcggtggcat tgggtcaaga gcgtggtcgc caccgcgccg ctgaagtgga
     1261 gggtcccctg gctctgacga tactcgacgc ggacgtggca cggggccacg atctcctcgc
     1321 cgaccggatc cagaaaggcc accgactcgt cgccctcgag gaccccgctc gtttcgacga
     1381 gctcgcgcag gtcgaccttc atctccgact cccacccggg cgcgtaacta ccgacacgat
     1441 gggcggttcc agccggacga atctaggccc caccgaacac aatttcaagc tctttctgcg
     1501 cgttctcggc agcgtccgag ccgtgtaccg cgttgttttg caagctctta ccgaacatgt
     1561 agcgcaccgt accggggcgc gcgtcggcgg ggttggtggc cccgatgaac tcgcggatgc
     1621 ggctgaccgc ctcgtcggct tcgatttcga tcgccaccac cggaccgctc gtgatgtatg
     1681 caaccaggtc cttgaagaac ggccgctcgc gatggacggc gtagaagcgc tcggcacttt
     1741 cgcgcgagaa ctgcgtcttg gccagccgcc gaatcgcgaa gcggttcttc tccaccagcc
     1801 acaggatgtc tccgacatga ccggcggcga cggcgtcggg cttgatcatt agaagggtct
     1861 tcatgggttc gatgtcctcc tggacgaatg cttacgcgcg cttgagcgac ggtgcggccg
     1921 aggcgaccag cgacgtgatt tccgacggca ccttcgcgac cggaatgccg accgccgcga
     1981 aggccgcgat cttgtcggcg gccgtgcccg agccgccggt gacgatggcg ccggcgtgcc
     2041 ccatgcgctt gcccggcggc gcggtttgtc cggcgatgaa cgacaccacc ggcttggtca
     2101 ccttctcttt gatgaatgcg gccgcctctt cttcgtcgga accgccgatc tcgccgatca
     2161 ggacgatcgc ttcggtttct tcgtcggctt ggaacaggcg cagcgcgtcg atgaagttgg
     2221 tgccgatcac cgggtcgccg ccgatgccga ggcacgtcga ctgccccatg ccggcgcgcg
     2281 tgagttggta gacgacttcg taggtcagcg taccgctgcg cgacaccacc ccgaccgagc
     2341 cgggatgcac gatgctcgcg gggagaatac cgactttgac gcgcgcgttg ggcgcgatca
     2401 ggcccgggca gttggggccg accaggcgcg tggcgcgctc tttcaagaac ggcacgacgc
     2461 gcagcatgtc gcgaaccggc acgccttcgg tgatacaaac cacgagacgg acgccggcgt
     2521 cggcggcctc gcagatggcg tcggcggcga acggcgcgcg cacgtagatc accgacgtgt
     2581 tggccccggt tgcgtcgacg gcttcgtgga cggagtcgaa gatgggaacg gtgttctcga
     2641 acgtggtacc gcccttgccc ggcgtcacgc cggccaccag gttggtcccg tacgccgcca
     2701 tctgcgacgc gtggaaagat ccgtcgcggc cggtgatgcc ctgcaccacc aggcgcgtgt
     2761 tgcgatcgat cagcatgctc acgtttatcg gtttcctttc gtcgaaatgg gccgacgcga
     2821 tcgatccctc gaacgcgatt tctgcagcgc gaataccacg cgatactgtc cgtctcgcgc
     2881 tgcagtttga gcggagaggg acgatcgcat cggccgccgt gcgctac
//
LOCUS       DASPGK010000152         5825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_977128, whole genome shotgun sequence.
ACCESSION   DASPGK010000152 DASPGK010000000
VERSION     DASPGK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977128"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..2356)
                     /locus_tag="VEC56_04390"
     CDS             complement(188..2356)
                     /locus_tag="VEC56_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV13422.1"
                     /translation="MVTRRDKRSRGAWWFAAGAALVIGLVVMASSNSVFAKKYVYPEA
                     MKQDVVDNYHGTQVADPYRWLEDPDSKDTMDWVAKENELTRSYIDSYAKRDAIEQRLT
                     KLWNYPKYGLPNKQGDRYFFSKNDGLQNQSVLYVQKSLDGEAKVVIDPNTLSADGTVA
                     LSGTNYTKDGTMMTYAVSASGSDWQEVKVRDIDSGKDNGDVLKWCRFSGIGWKKDKSG
                     FWYNRFPAEGEVPEEDRSNYNKVYWHKLGTPQSEDVLVYEDKANKELGFSPYVTEDGD
                     YLVLYVYHGTDPKNGIYYRKTDGTGDWVKLIEHGTAMFTPIDNIGTTWYFHTDLDASR
                     GRVFTMDITKPAKENWKEIIPQKTEVIDNVTMVNNELVVAYLKDARHQLMIFSKEGKM
                     RREIELPTIGTVGGVSGDREDTEMFFAFTSYTYPTTSFRYDFKTDKVEVFRKPEVDFD
                     GSQFETKQVFYPSKDGTKIPMFITHKKGVKLDGSNPTLLYGYGGFNISMTPTFSIGRV
                     MWLENGGVYAVACLRGGNEYGEEWHQQGVLDRKQNVFDDFIAAGEYLIKEKYTQTKLL
                     AINGGSNGGLLTAACMVQRPDLFGAVVCQVPVIDMLRYHKFTVGRYWVSDYGNAEENA
                     KDFAYMIKYSPLHNVKPGYKSPPVLITSADTDDRVVPAHAKKFAATLQANDAGDNPIL
                     IRIETKAGHGAGKPTSKQIEEAADIYSFLFKTLNVKPLTV"
     regulatory      2523..2611
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2665..3813
                     /gene="thiO"
                     /locus_tag="VEC56_04395"
     CDS             2665..3813
                     /gene="thiO"
                     /locus_tag="VEC56_04395"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HXV13423.1"
                     /translation="MFLDTTSIQMTGRRVVVVGGGVIGLSIAWRSARAGAHVTVIERD
                     RVGAGASRAAAGMLAPITEAGHGHGDQAMTRFARASLARYRSFAAELEADADSPVTLD
                     TRGTLIAALDRDDVEAVRRAFEHRRALGLPVEWLAGAGAREKEPLLSPRTSAAMWIPD
                     DHQVDTRGLLEALLRACTHRGVVIRESAEVSRVLVRDGAVAGVRTTHDDAEAEVVVMA
                     AGAWSGAIDGIPAGAVAPVRPVKGQIVRLRGAEHFPLAHVIRSPRVYVLPKTDGSVLV
                     GATQEEMGFDVRPTAGAVKDLLEHAWELLPAIYELAFEGVEVGLRPASRDHLPIIGPT
                     RVRGLVMATGHFRSGILLAPATADAVVEGIASGRFGDLVAAFSPERFG"
     gene            3816..4022
                     /gene="thiS"
                     /locus_tag="VEC56_04400"
     CDS             3816..4022
                     /gene="thiS"
                     /locus_tag="VEC56_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXV13424.1"
                     /translation="MEIRINGTMSTVAADSTVADVVHSLGVDEDTSGVAVAVNDSVLP
                     KRDWAARRLAAGDAVEVIRAVQGG"
     gene            4024..4812
                     /locus_tag="VEC56_04405"
     CDS             4024..4812
                     /locus_tag="VEC56_04405"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXV13425.1"
                     /translation="MTPANDNLVIAGREFRSRLIVGTSRYPDPHTMLEALEASGTQMV
                     TVAVRRVSLTDGSKENLLAMLRGRWSVLPNTAGCYTAKEAVLTAQLAREALDTNWIKL
                     EVIGDEETLFPDVPELLKAAHELLADGFVVLPYCNDDPITCRKLADMGCAAVMPLGAP
                     IGSGMGIRNPYNLRIIREQCRVPLIVDAGVGTASDVAVAMELGVDGVLLNTAIAQAQR
                     PVQMATAMRLAAEAGRLAYTAGRVPRRLYAKASSPEEGLIGRDE"
     gene            4815..5453
                     /locus_tag="VEC56_04410"
     CDS             4815..5453
                     /locus_tag="VEC56_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXV13426.1"
                     /translation="MVDFRLYLVTDRTRCAPRSLASIVHDACVSGVRAVQLREKDLSE
                     TDLVSAATRLSGLLRPRGAKLFVNVTPFVDRDTASLLAASPGVDGFHVPDDPALLTEF
                     RALFPKLLIGASTHSVDGVRNAAAAGADFLTFGPIFATRSKQNRPHGLDLLQSACRAV
                     SVPIFAIGGVTAENARACLDAGAHGVAVVGAVMEASSVRSAVRAFAEALGEL"
     gene            5450..>5825
                     /locus_tag="VEC56_04415"
     CDS             5450..>5825
                     /locus_tag="VEC56_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXV13427.1"
                     /translation="MSSIGRLHVITDVVVQDRYSHEELAEMACAGGADVVQLRDKLLA
                     RDAFAETARRVAAICRRHGVRLIVNDYADVAREVEADGVHLGRTDLSIREARALLGPR
                     AILGASTGTLEQAEQAEREGADY"
BASE COUNT          912 a         1872 c         1973 g         1068 t
ORIGIN      
        1 cgaattcgtt ggtcccaagg ctcgttgctt tcgacatgaa ctcctcccgc gctcgatcag
       61 ctacgtaacg acgcgggaac ttggatcaca actcctcacg ctgtcttaca cattcccggg
      121 caataaagca agcaggggcg gcccgccgcg cgagccgccc ctgcgacaaa agcacccgct
      181 cgcgacgcta gaccgtcaag ggtttgacgt tgagcgtctt gaacaggaac gagtagatgt
      241 cggccgcctc ctcgatctgc ttcgaggtgg gcttgccggc gccgtggccc gccttggtct
      301 cgatgcggat caagattgga ttgtcgccgg cgtcgttggc ctgcaaggtg gccgcgaact
      361 tcttggcgtg ggcggggacg acgcgatcgt cggtgtcggc gctcgtgatc aagaccggcg
      421 ggctcttgta gcccggcttt acgttgtgaa gcggcgagta cttgatcatg tacgcgaagt
      481 ccttggcatt ctcctcggcg ttcccgtagt cggagaccca gtagcgaccg accgtgaact
      541 tgtggtagcg cagcatgtcg atcaccggca cctggcacac caccgcgccg aacaagtcgg
      601 gacgctgcac catgcaggcc gcggtcaaca gacccccgtt gctgccgccg ttgatggcca
      661 ggagcttggt ctgcgtgtac ttctccttga tgagatactc gcccgccgcg atgaagtcgt
      721 cgaagacgtt ctgcttgcgg tcgaggaccc cctgctggtg ccactcttcg ccgtactcgt
      781 tgccgcctcg caggcaggcc accgcgtaca cgccaccgtt ctcgagccac atcacgcgcc
      841 cgatggagaa ggtcggcgtc atgctgatgt tgaaaccgcc gtagccgtat agcagcgtcg
      901 ggttgctgcc gtccagcttg acacccttct tgtgggtgat gaacatcggg atcttggtgc
      961 cgtccttgga cggatagaac acctgcttgg tctcgaactg ggatccgtcg aaatcgacct
     1021 cgggcttgcg gaacacttcg accttgtcgg tcttgaagtc gtagcggaac gacgtggtcg
     1081 ggtatgtgta cgaggtaaac gcgaagaaca tctcggtatc ctcgcgatcg cccgacacgc
     1141 cgcccacggt cccgatggtc ggcagctcga tctcgcggcg catcttgccc tctttgctga
     1201 agatcatcag ttgatggcgc gcgtccttca ggtaggccac caccagctcg ttgttgacca
     1261 tggtgacgtt gtcgatcacc tcggtcttct gcgggatgat ctccttccag ttctccttgg
     1321 ccggcttggt gatgtccatc gtgaacacgc ggccgcgcga tgcgtccagg tcggtgtgga
     1381 agtaccaggt cgtgccgatg ttatcgatgg gcgtgaacat cgcggtgccg tgctcgatca
     1441 acttgaccca gtcgccggtg ccgtcggtct tgcgatagta gatgccgttc ttggggtcgg
     1501 tgccgtgata gacgtagagc accaggtagt cgccgtcttc ggtcacgtac ggcgagaagc
     1561 cgagttcctt gttggccttg tcttcgtaga ccaggacgtc ttcggactgg ggcgtcccca
     1621 gcttgtgcca gtacaccttg ttgtagttgc tgcggtcctc ttcgggcact tcgccctcgg
     1681 ccgggaagcg gttgtaccag aagccgctct tgtctttctt ccagccgata ccggagaagc
     1741 ggcaccactt gagaacgtca ccgttgtcct tgccgctgtc gatatcgcgg accttgactt
     1801 cctgccagtc gctgccgctg gcggagacgg catacgtcat catggtcccg tctttggtgt
     1861 agttggtccc cgagagcgcg accgttccgt cggcgctcag cgtattggga tcgatgacga
     1921 ctttcgcttc gccatccagc gacttctgca cgtagagcac ggactggttc tgcagcccgt
     1981 cgttcttgga gaagaagtag cgatcgccct gcttgttggg gagtccgtac ttgggatagt
     2041 tccacagctt ggtcaagcgc tgctcgatgg cgtcgcgctt ggcgtacgaa tcgatgtagg
     2101 accgcgtcag ctcgttctct ttcgcgaccc agtccatagt gtccttggag tccgggtcct
     2161 cgagccagcg atacgggtcc gcgacttggg tgccgtggta gttgtccacg acgtcctgct
     2221 tcatcgcttc gggatacacg tacttcttcg cgaacacgga attcgaactc gccatgacga
     2281 ccaatccaat cactagtgcg gccccggccg cgaaccacca cgcgcctcgc gagcgcttgt
     2341 ctcgacgtgt caccatcttg tcctcctgct cgactggaat cgaaggaatc gttcgggtca
     2401 cctaagaaaa gggtgggtgg cgcgggccgc gaggggtcca cgccgaacgc ggggcattat
     2461 acacgaatat gggttcactt gacacccgcc gggcgggggc gtatcgtcgg ggccaagaca
     2521 catggggtgc tcgccgattt cgcggggaaa gcgcgccggc gggctgagat cagacccaac
     2581 gcacctgatc cgggtaatgc cggcgaaggg aaccctcgga acgaacgctg cgccgacttc
     2641 cgctcgccgg gtgcgaagtc ggcgattttt ttggatacca ccagcataca aatgacgggg
     2701 cgacgcgtgg tcgtggtcgg gggcggcgtg atcggtctct cgatcgcgtg gcggtcggcc
     2761 cgagccggcg cgcacgtgac cgtgatcgag cgcgatcggg tcggcgcggg tgcgagccgt
     2821 gcggccgcgg gcatgctggc gccgatcacc gaggccggcc acgggcacgg cgaccaagcc
     2881 atgacgcgct tcgcgcgcgc gagcctcgcg cgctatcgct ccttcgcggc cgagctggaa
     2941 gcggacgccg attcaccggt caccctcgac acccgcggca cgctgatcgc ggccctcgac
     3001 cgcgacgacg tcgaagcggt gcggcgcgcg ttcgagcacc ggcgcgcgct cgggctcccg
     3061 gtcgaatggc tcgcgggtgc gggtgcgcgc gagaaggagc ccctgctctc tccgcgcacg
     3121 agcgccgcga tgtggatccc ggacgaccac caggtcgaca cgcgcggttt gttggaagcc
     3181 ttgctgcgcg cgtgcacgca ccgcggcgtc gtgattcgcg agtcggcgga ggtctcgcgc
     3241 gtgctcgtgc gcgacggcgc ggtcgcaggc gtgcgcacga ctcatgacga cgccgaggcc
     3301 gaggtcgtgg tgatggctgc gggcgcgtgg tcgggagcca tcgacggtat tccggccggc
     3361 gcggttgcgc cggtgcggcc ggtcaaagga cagatcgtgc gcctgcgcgg cgcggagcac
     3421 ttccccctgg cacacgtgat acgctcgccg cgcgtctatg tgctccccaa gaccgacggc
     3481 agcgtgctgg tgggagccac ccaggaagag atgggcttcg acgtgcgccc gaccgcgggt
     3541 gccgtgaagg acttgctcga gcacgcgtgg gagctcctcc ccgcgatcta cgagctcgcg
     3601 ttcgaagggg tcgaggtggg gctgcgaccg gcgtcgcgcg accacctgcc catcatcgga
     3661 ccgacgcgcg tgcgcgggct ggtcatggcg acgggacatt ttcgaagcgg catcctgctg
     3721 gcaccggcga ccgcggacgc ggtcgtcgaa gggatcgcga gcgggcgttt cggcgacctg
     3781 gtggcggcgt tttcgccgga gagattcgga taacgatgga gatcaggatc aacggcacga
     3841 tgtcgacggt cgcggcggat tccaccgtcg ccgacgtcgt gcactcgctc ggcgtcgacg
     3901 aggacaccag cggtgtcgcc gtcgcggtga acgattccgt tctgcccaag cgcgactggg
     3961 ccgcgcgccg cctcgcggcg ggcgacgcgg tcgaggtcat tcgcgcggta caaggaggat
     4021 agcgtgacac ctgccaacga caacctcgtc atcgcgggcc gcgagtttcg ttcgcgcttg
     4081 atcgtgggaa cgtcgcgcta ccccgacccg cacaccatgc tggaggcact cgaggcgagc
     4141 gggacacaga tggtcacggt cgcggtgcgg cgcgtgagcc tcaccgacgg ctccaaagag
     4201 aatctgctcg cgatgctgcg cgggcgctgg tcggtgctgc ccaacacggc cgggtgctac
     4261 accgccaagg aggcggtctt gaccgcgcag ctcgcgcgtg aagcgctcga caccaactgg
     4321 atcaaactcg aggtgatcgg cgacgaagag acgctctttc ccgacgtccc cgagctcttg
     4381 aaggccgcgc acgaactgtt ggccgacggg ttcgtggtac tcccctactg caacgacgac
     4441 cccatcacgt gccgcaagct ggccgacatg gggtgcgccg cggtgatgcc gctgggtgcg
     4501 cccatcggct ccgggatggg gattcgcaac ccgtacaacc tgcgcatcat tcgggagcag
     4561 tgccgcgtcc cgctgattgt cgacgcgggc gtgggaaccg cgtccgacgt cgcggtggca
     4621 atggagctcg gcgtcgacgg cgtgctcttg aacaccgcca tcgcgcaggc gcagcgcccg
     4681 gtgcaaatgg cgaccgcgat gcgcctggcc gcggaagcgg gccgtctggc ctacacggcg
     4741 ggccgcgtcc cgcgccgtct gtacgcgaag gcgtcgagcc cggaagaggg actcatcggg
     4801 cgcgacgagt gacgatggtc gactttcgcc tctatctggt caccgaccgc acgcgctgtg
     4861 cgccgcgctc gctcgcgtcc atcgtccacg acgcgtgcgt gtccggggtg cgcgcggtgc
     4921 agttgcggga gaaagacctc tccgagaccg acctcgtgag cgcggccacg cgcctttccg
     4981 gattgctgcg gccgcgcggc gccaagctgt ttgtcaacgt cacccccttc gtcgaccgcg
     5041 acacggcgtc gctcctggcc gcgagtcccg gcgtggacgg attccacgtg ccggacgatc
     5101 ccgctctcct gaccgagttc cgcgcgttgt tcccgaagtt gctgatcggc gcgagcacgc
     5161 actccgtcga tggcgtccgc aacgcggcgg cagcgggcgc tgactttctc actttcggtc
     5221 ccatcttcgc gacgaggtcc aaacagaacc gcccacatgg actcgacctc ctgcagagcg
     5281 cgtgcagggc ggttagtgtc ccgatattcg cgatcggcgg tgtgacggcc gagaacgcgc
     5341 gcgcgtgcct cgacgccggt gcgcacgggg tggcggtggt gggtgccgtc atggaggcgt
     5401 cgagcgttcg cagcgccgtg cgcgccttcg ccgaggccct gggagagctg tgagctccat
     5461 cggtcgcctg cacgtcatca ccgacgtcgt cgtccaggat cgctactcgc acgaagagct
     5521 ggccgagatg gcgtgcgcgg gaggcgcgga cgtcgtgcag ctgcgcgaca aattgctcgc
     5581 ccgcgacgcc ttcgcggaga ccgcgcgacg cgtggccgcg atctgtcgcc gacacggagt
     5641 ccgcttgatt gtcaacgatt acgccgacgt tgcgcgcgag gtcgaggcgg acggcgtgca
     5701 cctggggcgc accgacctgt cgatccgcga ggcgcgcgcg ctgttggggc cgcgcgcgat
     5761 cctcggcgcg agcaccggca ccctcgaaca ggccgagcag gcggaacgag agggcgcgga
     5821 ctacg
//
LOCUS       DASPGK010000153         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1328344, whole genome shotgun sequence.
ACCESSION   DASPGK010000153 DASPGK010000000
VERSION     DASPGK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328344"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VEC56_04420"
     CDS             <1..1641
                     /locus_tag="VEC56_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13428.1"
                     /translation="IEREFAYDGAGPVTLQFTCTYDLEPGYDFARALIEVNGTESVLL
                     THTGSGSGTANVPLAGALAPLAGAGGTYTVKFRVTSDFSYDDTDGQNPTTCGAFVVDN
                     VSVNGGGESYATDFESNVGGWHQDPAENPASEYWLVENRRRVGFDQGLREQGLLIWHV
                     DDEVMRAWAQVNNAVLTDVRGLVLEEADGQFDLNNGINSNTGEAADVFPGPLAKTSFT
                     SATSPNSDDNTGRDTQIAVTSIGAPAATMTATLRAGDPAPTAASVAPNNIDNDQVAVV
                     VDVAGTRIRHGATFRFVKSGTVATNGTRDSEDVVATSLEWIDPTLLRGTVNVYSKESG
                     AWDLVVTNPDGQTVTVLNAVTLNLIVATKLLSAAIDVAADGVRLRYELLGREAGEELR
                     LYRSQRANDGWVKIADLPASTTEFYEYLDADVEAGRTYYYLLESVIDGESRELHRGVA
                     IVPARELVLEQNHPNPFNPRTAIRFYLPERGPVELDVYDVRGALVRRLARGDFDSGPH
                     EVDWDGTDAHGQPVASGMYVYRLVTERRALSKKMMLLK"
     gene            complement(1667..>2903)
                     /locus_tag="VEC56_04425"
     CDS             complement(1667..>2903)
                     /locus_tag="VEC56_04425"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HXV13429.1"
                     /translation="RFASRHALKGTAGTLRLVTYMITVEDAAGNRGRYYAERSVYAHP
                     PVGILARFPRGRVITGARVPIEIDLTQRARNAEPTRYYWFSPSGLRLAEGNQGSFAAG
                     PDDTSGATLHVEIPTPCRPGVFPFTLKFLNGEREAGTIQASLFKPYQWAFLGPFAGGT
                     LDKKFPPENGIALLNAYNAGKRKVQWAPVPAGATGPRGEVVMRKLVDEPGVSYLYTVV
                     SVANETDINARLVSNCPAALFLNGRRVLANPGGAGDSTAAMVHLHADKNHILVKVAGD
                     AASTVSFGLGDDNNIAADEFNNDLVELVEGYQELLARAEASEEVPSEARRLVTFTYSD
                     PNASAVSVVGSFNGWSPETHRLQKTAEGRWELTLSLAPGRYSYRFLIDQKKQVLDPST
                     AMTEPDGYGGKNSVVVVKR"
BASE COUNT          485 a          982 c          967 g          469 t
ORIGIN      
        1 atcgagcgcg agttcgccta cgacggcgcc ggacccgtga cgctgcagtt cacgtgcacc
       61 tacgatctcg agcccggcta cgatttcgca cgcgcgttga tcgaggtcaa cgggaccgag
      121 tcggtgctgc tgacgcacac cggcagcggc agtgggaccg ccaacgtgcc gttggccggc
      181 gcgctggcgc cgctcgccgg cgcgggcggg acgtacacgg tcaagttccg tgtcactagc
      241 gatttctcct acgacgatac cgacggacag aaccccacga cgtgcggtgc gtttgtcgtc
      301 gacaacgtga gcgtcaacgg cggcggcgag tcgtacgcca ccgatttcga gtccaatgtc
      361 ggcgggtggc accaggatcc cgccgagaac ccggcctcgg agtactggct ggtcgagaac
      421 cggcggcgcg tcggcttcga ccaggggctg cgcgaacaag gactcttgat ttggcacgtg
      481 gacgacgaag tgatgagggc gtgggcccag gtcaacaacg ccgtgctgac cgacgtgcgc
      541 ggcctggtac tcgaagaagc cgacggccag ttcgacctga acaacggcat caactccaac
      601 acgggcgagg ccgcggacgt gttcccgggt ccgcttgcga agacctcctt cacctcggcc
      661 acctctccga acagcgacga caacaccggg cgcgacaccc agatcgcggt gacgtccatc
      721 ggcgcgccgg ccgccaccat gacggcgacg ctgcgcgccg gcgaccccgc gcccaccgcc
      781 gcgtcggtgg cgcccaacaa catcgacaac gaccaagtcg cggtcgtggt cgacgtcgcg
      841 ggcacgcgca tccgtcatgg cgcgacgttc cgcttcgtca agtccggcac cgtggccacg
      901 aacggcaccc gcgacagcga agacgtggtc gcgacctcgt tggagtggat cgaccccacg
      961 ctcctgcgcg ggacggtcaa cgtgtactcg aaggagtcgg gcgcgtggga cttggtcgtg
     1021 accaatccgg acggacagac ggtcacggtc ctgaacgcgg tgacgctgaa cctgatcgtc
     1081 gccaccaagc tcttgtccgc ggccatcgac gtcgccgccg acggcgtgcg gttgcgctac
     1141 gaactcctgg gacgcgaagc gggcgaagag ctgcgcctct accgatcgca gcgcgcaaac
     1201 gacggttggg tgaagatcgc cgacctgccg gcgtccacca ccgaattcta cgagtacctg
     1261 gacgccgacg tcgaagccgg acgtacctac tattacttgc tggagtccgt gatcgacgga
     1321 gaatcgcgcg agctgcaccg gggcgtggcg atcgtgccgg cgcgcgaact ggtgctcgag
     1381 cagaaccacc ccaatccctt caacccgcgc accgcgattc gcttctatct gccggagcgc
     1441 ggacccgtgg agttggacgt ttacgacgtg cgcggtgccc tcgtgcgccg gctcgctcgc
     1501 ggcgacttcg attcgggacc gcacgaggtg gactgggacg gcaccgacgc gcacgggcag
     1561 ccggtcgcgt cgggaatgta cgtctatcgt ctggtgacgg agcgccgcgc gctctcgaag
     1621 aagatgatgc tgctgaagta ggattgcggc aggcagcgtt cgcgcgttag cgcttcacca
     1681 ccacgaccga gttcttgccg ccgtagccgt ccggctccgt catcgccgtc gacggatcca
     1741 gcacttgctt cttctgatcg atgaggaatc gatacgaata gcgtcccggg gccagcgaca
     1801 gcgtgagttc ccaccggcct tcggccgtct tctggagccg gtgcgtctcg ggcgaccagc
     1861 cgttgaagct ccccaccacc gaaaccgcgc tcgcgttcgg gtccgagtag gtgaacgtca
     1921 ccaagcgacg cgcctcactc ggtacttctt cgctcgcttc cgcgcgcgcc aagagctcct
     1981 gatagccttc gaccaattcg accaggtcgt tgttgaattc gtcggccgcg atgttgttgt
     2041 cgtcgccgag tccgaacgac accgtggagg ccgcgtcgcc cgcgaccttg accaggatgt
     2101 ggttcttgtc ggcgtgcagg tggaccatgg cggcggtcga gtcgcccgca ccgcccgggt
     2161 tggcgagcac gcggcggccg ttgaggaaca gcgccgcggg acagttcgac accaggcgcg
     2221 cgttgatgtc ggtttcgttc gcgaccgaga ccaccgtgta caggtagctg acacccggct
     2281 cgtcgaccag cttgcgcatc acgacctcgc cgcgcggtcc cgtggcaccc gcgggaaccg
     2341 gcgcccactg gaccttgcgc ttgcccgcat tgtacgcgtt caagagcgcg atgccgttct
     2401 cgggcgggaa tttcttgtcg agggtgccgc ccgcgaacgg ccccaggaac gcccactggt
     2461 agggtttgaa caagctggcc tggatcgtcc cggcttcgcg ctccccgttt agaaacttca
     2521 aggtgaacgg aaacacgccc ggccggcaag gggttgggat ctcgacgtgg agcgtcgcgc
     2581 cggaagtatc gtccggcccg gccgcgaacg acccctgatt gccctcggcc aggcgcagcc
     2641 cgctcggact gaaccagtag tagcgcgtgg gctccgcgtt gcgcgcgcgc tgcgtcaggt
     2701 cgatctcgat ggggacgcgc gcgccggtga tcacgcgccc gcgcgggaag cgcgccagaa
     2761 tgccgaccgg cgggtgggcg tacacggacc gctcggcgta gtaacgcccg cgattcccgg
     2821 ccgcgtcctc caccgtgatc atgtaggtga ccagccgcag cgtgcccgcc gttcccttga
     2881 gcgcgtgacg cgaggcgaat cgc
//
LOCUS       DASPGK010000154         7480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_509642, whole genome shotgun sequence.
ACCESSION   DASPGK010000154 DASPGK010000000
VERSION     DASPGK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509642"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VEC56_04430"
     CDS             complement(<1..544)
                     /locus_tag="VEC56_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV13430.1"
                     /translation="MGEFVPSRAHEHDARVLSNLRLSRRYFLLRLERPRDFIEPAAGQ
                     FVHVAVPTPDDRFFLRRPFSIHDCTRDAIELVIVEVGPGSQALRRAREGDTIRFYGPL
                     GHAYPVLPDKRILAIGGGVGLAPLYFYGFRAPGRIGNGYRLLYGARTREDLFLEHVAL
                     EGKGVSLSTDDGSYGFKGNVV"
     gene            complement(546..2183)
                     /locus_tag="VEC56_04435"
     CDS             complement(546..2183)
                     /locus_tag="VEC56_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13431.1"
                     /translation="MIARRIVTAAAAALALLGASCAYYNTLYNAKEKYREAEKAQDRS
                     ATAQVASGVAPGSTTPGRNPQAAQYEDVVEKCKNVIATYPKSRHVDDAMLLSAQALYR
                     LAQFDEAVAALDTLETRYPKSDLLDDAAFLKGKSLVAAEKYDLAVSVLRAYVDRYRKH
                     DDRAEGLYLLCISLMQMGLNDDAVATLGRLEKDHGRSDYRFRAQVDMAEILAGKELYK
                     ESLAIYERLGESRIPESVRFDVLIGMARVQEHLGEYANALETLRRIRKIPPGSSKEPE
                     LVLLRAGAFAGIDSTTRAINTYKDVTKRFARGVYAAEAHFRLGEIYEGMDSLQTAQTN
                     YQEVPRAYSGSPFAEDAIKRSSNIGRILKLEQTSGDDSPEAIAMRTFSMAEIQLLQFN
                     SAEKAIPSYEKVAADYPDSEYAPRAVYALGYIHGVVLGDSVKAREWYDVLLAKYPESQ
                     QAQFAYAFYKGATPPPPYSDLMKTATAVKPAAAQQRPPRREPPRVAPTRPAATDTTRT
                     PTQPPAGATPAGAVPTSPTSAPRDTSTAPSDTTQGGG"
     gene            complement(2185..3507)
                     /locus_tag="VEC56_04440"
     CDS             complement(2185..3507)
                     /locus_tag="VEC56_04440"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXV13432.1"
                     /translation="MMLKLMNLNHTSYIIDNVVVVDPVRNTSYPGHVAVVEGKIDAVE
                     RGRAKEASLPIYDGGGLHLAPGFVDIHVHLREPGYEYKETIASGTLAAVAGGFTAVAC
                     MPNTNPAIDDPSVVEFILKQAKIAGRARVYPIAAATKGRKGEILAEYHELVAAGAVAV
                     TDDGAPVASAGMARRVMEYAAPLGIPFIEHCEDMAASGEGVMHEGFYSTKLGLKGIPA
                     HSEEVCLARDLLILKSVRGAKFHAAHMSTKGSINLLRRAKEDGLPVTAETAPHYLCLE
                     DKDLVTYDTNKKINPPLRGEDDREAMIEALKDGTIDCVASDHAPHAAHEKQVEFNAAP
                     NGSVGLETMFPLLVKHLVKPGHLTLPQVVALITHKPAQCLGIPGGTLTKGAAADLVLF
                     DPGEEWTVRADALHSKSRNSAFIGHTITGRVKHTLVDGVSVLPAKVGV"
     gene            complement(3504..4493)
                     /locus_tag="VEC56_04445"
     CDS             complement(3504..4493)
                     /locus_tag="VEC56_04445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXV13433.1"
                     /translation="MTPMTRKHLLGLEGMSADDLKRFLDTAESFKEVSERPVKKVPAL
                     RGTTIANLFFEPSTRTRISFELAEKRLSADSVNFTVSGSSVSKGETLRDTAENIQAMK
                     VDMIVIRHSAAGAPHFLASLLEASVVNAGDGAHEHPTQGLLDMFTLRERWGSFDDRKV
                     VIVGDLAHSRVARSNIHGLVTMGVEVVVCGPPTLIPTDIERLGVSVETDLRKALEDAD
                     AVNVLRIQKERQAAGLFPSIDEYARVWGVNADALKRAKPDCVVMHPGPMNRGVEIAQD
                     VADGERSVILDQVTNGVAVRMAVLYLCNGGVAIESEEEQAATDAKATKGKQVA"
     gene            complement(4490..5041)
                     /gene="pyrR"
                     /locus_tag="VEC56_04450"
     CDS             complement(4490..5041)
                     /gene="pyrR"
                     /locus_tag="VEC56_04450"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXV13434.1"
                     /translation="MSEIVYKSTLLDRDSIRRAVKRIAHEIVERNGGVDGLVVVGIRR
                     RGVPLAERIAQYIEQIEGRAVPVGALDITLYRDDFQVVTKQPHVGKTEIKDDITDKIV
                     ILVDDVLYTGRTIRAALDELIDFGRPKRIQLAVLIDRGHREFPIKADYVGKNVPTSET
                     ELVEVHLEEIDQADEVLLAEKQP"
     gene            complement(5284..5823)
                     /locus_tag="VEC56_04455"
     CDS             complement(5284..5823)
                     /locus_tag="VEC56_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV13435.1"
                     /translation="MNAYGVIVGPGTVRFERLLPGPIERVWSYLTDSEKRGTWLAKGN
                     MDLRIDGNVEFEFCNSQLTGHDETTPAKFAHVDTEKRMTGRVVACEPPRLLTYTWGEN
                     FGENSEVTFELAPRGDKVLLTLTHRRLGTRDTMVMVGGGWHTHLDILADRLEQRKPPA
                     FWATYLEREAEYEKRVPTT"
     gene            complement(5820..6155)
                     /locus_tag="VEC56_04460"
     CDS             complement(5820..6155)
                     /locus_tag="VEC56_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV13436.1"
                     /translation="MEIFHALADPTRRRIVELLGRGEHTAGAIGEKFAVSAPAISQHL
                     KVLREAQLVRVRAQGQHRIYSIDPAGLAELDAWLQRTRSFWAKSLDALERELAKPAVT
                     PKNKTRRKR"
     gene            complement(6201..6749)
                     /locus_tag="VEC56_04465"
     CDS             complement(6201..6749)
                     /locus_tag="VEC56_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13437.1"
                     /translation="MHIADAQREVRTVFQGGFVGSLVSAALWGTSTALATVRSPRAGI
                     IALTLGGVLIFPVTSALLKLTQGRGALSKENPLDKLAMQVAFTIPFALPVVGGAALYK
                     LSWFYPAMLVIVGAHYLPFVFLYGMRAFYVLGGGMIMAGVMIGLYANESFTLGGWVGT
                     AMFVVFAFVAKAIVGREARTQG"
     gene            complement(6761..>7480)
                     /locus_tag="VEC56_04470"
     CDS             complement(6761..>7480)
                     /locus_tag="VEC56_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV13438.1"
                     /translation="SKLRTDRATRKPVKLILFDIDGTLVDCGGQARVAFATALNAVFG
                     ESGPIDEYDFSGKTDTRIVVDLMTMAGRPRDEILATLPRLKDAYLDTLDRSLERERMR
                     VLPGIEPLLEHLAARDDIVLALLTGNFERGARIKLSRHDLNRYFGFGSYGDNVFDRND
                     LPPIALERALLATQRRFRPDETVIIGDSVLDVGCARAHGIPCLAVATGRTSRSDLEAV
                     APSWIVDDLAGVLDHPAFSPR"
BASE COUNT         1155 a         2415 c         2527 g         1383 t
ORIGIN      
        1 gcaccacgtt gcccttgaat ccgtagctcc cgtcgtcggt cgatagcgag acccccttgc
       61 cttcgagggc cacgtgctcg aggaagagat cctcgcgcgt gcgcgcgccg tacagcaagc
      121 gatagccatt tccgatgcga cccggcgcgc gaaatccgta gaagtaaagc ggcgccaacc
      181 ccactccccc gccgatcgcg aggatgcgct tgtccggcag cacggggtac gcgtgaccga
      241 gcggtccgta gaagcggatg gtgtcgcctt cgcgcgcgcg gcgcagcgcc tgcgaacccg
      301 ggcccacttc gacgatcaca agctcgatgg cgtcgcgcgt gcaatcgtgg atactgaacg
      361 gccggcgcag gaagaagcgg tcgtcgggcg tggggaccgc gacgtgcacg aattgccccg
      421 cggccggttc gatgaagtca cgcgggcgct cgagtcgaag caggaagtag cggcggctca
      481 ggcgtaggtt ggaaagaacg cgcgcgtcgt gctcgtgcgc gcgcgagggg acaaactcac
      541 ccatgctaac cgccaccttg cgtggtgtcg ctgggcgcgg tgctggtgtc gcgcggcgcg
      601 gaggtcggcg acgtgggcac ggccccggcg ggcgtcgcgc ccgccggcgg ttgcgtcggc
      661 gtgcgcgtcg tgtccgtcgc ggccggccgc gtcggagcga cccgcggcgg ttcgcgccgc
      721 ggcggccgct gttgcgcggc ggccggcttc accgcggtgg ccgtcttcat gagatccgag
      781 tacggcggcg gcggcgtggc acctttgtag aacgcgtagg cgaattgtgc ctgctgactc
      841 tcgggatact tggcgagcag cacgtcgtac cactcgcgcg ccttgaccga gtctccgagc
      901 accacgccgt ggatgtagcc cagcgcgtat accgcgcgcg gcgcgtactc gctgtccggg
      961 tagtcggccg ccaccttctc gtagctgggg atcgccttct cggcgctgtt gaactgcagg
     1021 agctggatct ccgccatcga aaaggtgcgc atcgcgatcg cttccgggct gtcgtcgccc
     1081 gaggtctgct cgagcttgag aatacgcccg atgttcgagc tgcgcttgat cgcgtcctcg
     1141 gcgaagggcg aacccgagta cgcgcgcggg acttcctgat agttggtctg cgcggtctgt
     1201 agactatcca ttccttcgta gatctcgccc aggcggaagt gcgcctcggc ggcgtacacg
     1261 ccgcgcgcga agcgcttggt gacgtccttg tacgtgttga tcgcccgggt cgtcgaatcg
     1321 ataccggcga acgcgcccgc gcgcaagagc accagctcgg gctccttgct ggagcccggc
     1381 ggtatcttcc ggatgcgccg cagcgtctcg agtgcgttcg catactctcc gaggtgctcc
     1441 tggacgcgcg ccattccgat cagcacgtcg aagcgcacgc tctcggggat gcgtgattcg
     1501 cccaggcgct cgtagatcgc gaggctctcc ttataaagtt cctttccggc caggatctcg
     1561 gccatgtcga cctgggcccg gaagcgatag tcggatcggc cgtggtcctt ctccagccgc
     1621 cccagtgtcg ccaccgcgtc gtcgttcaat cccatctgca tgaggctgat gcacaggaga
     1681 tacagccctt cggcgcggtc gtcgtgcttg cgataccgat cgacgtaggc ccggagcacg
     1741 gagacggcga ggtcgtactt ctcggcggca accagactct tgcccttgag gaaggccgcg
     1801 tcgtcgagca ggtcgctctt cggatagcgg gtctccagcg tgtcgagagc ggccaccgct
     1861 tcgtcgaact gagccaagcg atagagcgcc tgcgcggaga gcagcatggc gtcgtcgacg
     1921 tgacggctct tgggatacgt cgcgattacg ttcttgcact tctcgaccac gtcttcgtac
     1981 tgcgcggcct gcgggttgcg gcctggtgtc gtggacccgg gcgcgacccc gctcgcgacc
     2041 tgcgccgtcg cggagcggtc ctgcgccttc tcggcttcgc gatacttttc cttcgcgttg
     2101 tagagcgtgt tgtagtaggc acagctcgcg cccaagagcg cgagggccgc cgcagccgcc
     2161 gtgacgatgc gtcgcgcgat catgctacac accaactttc gcggggagga cggagacgcc
     2221 gtcgaccagc gtgtgcttga cgcggcccgt tatggtgtgg ccgatgaacg ccgagttgcg
     2281 cgacttcgag tggagcgcgt ccgcgcgcac cgtccattcc tcgccggggt cgaagaggac
     2341 caggtcggcg gccgcgccct tggtcagcgt gccgcccgga atccccaggc actgcgcggg
     2401 cttgtgggtg atgagcgcga ccacctgcgg gagggtcaag tgtcccggct tcaccagatg
     2461 cttcaccagg agcgggaaca tcgtctccag gccgaccgat ccgttgggcg ccgcgttgaa
     2521 ctcgacctgt ttctcgtgcg cggcgtgggg cgcgtggtcg gaggccacgc agtcgatggt
     2581 gccgtccttg agcgcttcga tcatcgcttc gcgatcgtcc tcgccgcgca gcggcgggtt
     2641 gatcttcttg ttggtgtcgt aggtgaccaa gtccttgtcc tcgagacaaa gataatgcgg
     2701 cgcggtctcc gccgtaaccg ggagtccgtc ttctttcgcc cggcgtagca gattgatcga
     2761 acccttggtc gacatgtggg cggcgtggaa cttcgcgccc cggaccgact tcaagatgag
     2821 caagtcgcgc gcgaggcaca cttcctcgct gtgcgcggga attcccttca acccgagctt
     2881 ggtcgagtag aagccttcgt gcatgacgcc ttcgccggaa gcggccatgt cttcgcagtg
     2941 ctcgatgaac gggattccga gcggggccgc gtactccatc acgcgccgcg ccatcccggc
     3001 actcgccacc ggcgcgccgt cgtcggtgac cgcgaccgcg ccggccgcga ccaattcgtg
     3061 atactccgcg aggatctcgc ccttgcgccc cttggtggcg gccgcgatcg gatacacgcg
     3121 cgcgcgcccc gcgatcttcg cctgtttcaa gatgaactcg accacgctgg ggtcgtcgat
     3181 ggccgggttc gtgttgggca tgcacgcgac cgccgtgaag ccgcccgcca ccgcggccag
     3241 cgtccccgac gcgatcgtct ccttgtactc gtagcccggt tcgcgcaggt gcacgtggat
     3301 gtcgacgaaa ccgggcgcga ggtgcaatcc gcctccatcg tatatgggaa gcgacgcttc
     3361 cttggcgcgg ccgcgctcga ccgcgtcgat cttaccctcc acgaccgcga cgtgacccgg
     3421 gtacgacgtg ttgcgcaccg ggtccaccac gaccacgttg tcgatgatgt aggacgtgtg
     3481 attgagattc atgagcttca acatcatgct acctgcttgc ccttggtcgc cttggcgtcg
     3541 gtggccgcct gctcttcttc cgactcgatc gcgacgccgc cgttgcacag gtacaagacc
     3601 gccatgcgca ccgcgacgcc gttggtcacc tggtccagga tcacggagcg ctcgccgtcg
     3661 gcgacgtctt gcgctatttc gacgccgcgg ttcatcggcc ccgggtgcat gacgacgcag
     3721 tccggctttg cgcgcttcaa ggcgtcggcg ttgacacccc acacgcgcgc gtattcgtcg
     3781 atgctgggga agaggcccgc cgcctgccgc tccttctgga tgcgcagcac gttgaccgcg
     3841 tcggcgtctt cgagtgcctt gcgcaggtcg gtctcgacgc tgacgcccag tcgctcgatg
     3901 tcggttggga tcaacgtagg cggtccacat accacgactt caactcccat cgtgacgaga
     3961 ccgtggatgt tggagcgcgc gacccggctg tgcgccaaat cgccgacgat gacgaccttg
     4021 cggtcgtcga acgaacccca gcgctcgcgc agcgtgaaca tgtcgaggag gccctgggtg
     4081 gggtgctcgt gggcgccgtc gcccgcgttg accaccgagg cttcgagcag gctcgcgagg
     4141 aagtgcggcg cgcccgcggc cgagtgtcgg atcacgatca tgtcgacctt catggcctgg
     4201 atgttttcgg cggtgtcgcg caacgtctcg cccttggaca cgctgctgcc gctcacggtg
     4261 aagttcacgc tgtccgcgct caagcgcttt tccgccagct cgaagctgat gcgcgtgcgc
     4321 gtgctgggtt cgaagaagag gttcgcgatc gtggtgccgc gcagcgcggg caccttcttg
     4381 acgggacgtt cggagacttc cttgaaggac tcggcggtgt cgaggaagcg cttcaagtcg
     4441 tccgcgctca tgccttccag gccgagcaag tgcttgcgag tcatcggggt catggttgtt
     4501 tctccgcgag aaggacttcg tccgcctggt cgatctcttc cagatgaact tccaccaact
     4561 cggtctccga cgtgggcacg ttcttgccga cgtagtcggc cttgatggga aactcgcggt
     4621 ggccgcggtc gatcaaaacc gcgagctgga tgcgcttggg ccgaccgaag tcgatcagct
     4681 cgtcgagtgc cgcgcgtatg gtgcggccgg tgtagaggac gtcgtcgacc aggatcacga
     4741 tcttgtcggt gatgtcgtct ttgatttcgg tttttcccac gtgcggttgc ttggtgacga
     4801 cctggaagtc gtcgcgatag agcgtgatgt cgagcgcgcc caccgggacc gcgcgtccct
     4861 cgatctgctc gatgtattgc gcgatgcgct cggccagcgg caccccgcgg cgccggatgc
     4921 cgaccaccac cagtccgtcg acgcccccgt tgcgctccac gatctcatgg gcaatgcgct
     4981 tgacggcgcg acgaatggag tcgcggtcga ggagggtgga cttgtagacg atttcgctca
     5041 tgcctgcctt tcgggcggcg cgggaggcgg ttcggaggga ataagaaagc cctccggggg
     5101 attcagaggg cgagcgcgaa gcagcggcga cgaacgccga tgtgtggatt ctcgacccct
     5161 tgctggcctc ccggaccagc tttaaaagga tctgtcccga ggatggtagg gcttcggggg
     5221 ccgggaggca aggggtttct tgttcgtgtg ctcccggccc cgatcgtgct tcgtggtgcc
     5281 cgcctaggtg gtcggtactc gcttctcgta ctccgcttcc cgctcgaggt aggtggccca
     5341 gaacgcgggc ggcttccgct gctccagccg gtccgcgagg atgtccaggt gcgtgtgcca
     5401 gccccctccg accatgacca tagtgtcgcg cgtccccagc cggcgatgcg tgagcgtgag
     5461 cagcaccttg tcgccgcgcg gggcgagctc gaaggtcacc tcggagttct cgccgaagtt
     5521 ctcgccccag gtgtacgtca agaggcgcgg cggctcgcac gcgacgacgc gtccggtcat
     5581 gcgcttctcc gtgtcgacgt gggcgaactt cgcgggcgtc gtttcgtcgt ggccggtcag
     5641 ctgggagttg cagaactcga actcgacgtt cccgtcgatg cgcaggtcca tgttgccctt
     5701 cgcgagccag gtgccgcgct tctccgaatc ggtgagataa ctccacacgc gctcgatcgg
     5761 gccgggcaag agccgctcga agcggaccgt gccggggccg acgatgactc cgtaggcgtt
     5821 catcgctttc tccttgtctt gttcttgggt gtaacggcgg gcttggcgag ctcgcgctcg
     5881 agggcatcga ggctcttggc ccagaacgac cgcgtgcgct ggagccacgc gtcgagctcg
     5941 gccagcccgg cgggatcgat gctgtagatg cgatgctgcc cttgcgcgcg cacgcgcacc
     6001 aattgtgcct cgcgcaggac cttgaggtgc tgagagatgg cgggcgcgct gacggcgaat
     6061 ttctcgccga tggcacccgc ggtgtgctca ccgcgaccca gcagctcgac gatgcggcgc
     6121 cgggtcgggt cggccaaggc atggaagatt tccatgcggg gatatttgca tatctactta
     6181 attaagtcaa gaggaaattt ctagccctgg gtccgggcct cgcgccccac gatggccttc
     6241 gccacgaagg cgaacacgac gaacatcgcg gtgcccaccc agccgcccag ggtgaaggat
     6301 tcgttcgcgt acagcccgat catgacgccg gccatgatca tcccccctcc gaggacgtag
     6361 aacgcgcgca tgccgtagag aaacacaaac ggcaagtagt gcgcgccgac gatgacgagc
     6421 atcgcggggt agaaccagct gagcttgtag agcgcggcgc cccccaccac gggcaaggcg
     6481 aacggaatcg tgaacgcgac ctgcatcgca agcttgtcca acgggttctc cttggacagc
     6541 gcaccgcgtc cttgcgtcag cttgaggagc gcgctcgtga ccgggaagat gaggacgcct
     6601 cccagcgtga gcgcgatgat ccccgcgcgc ggggatctca cggtggcgag cgccgtcgat
     6661 gtcccccaca gcgcggcgga caccaagctg ccgacgaagc ctccctggaa cacggtgcga
     6721 acttcgcgtt gggcgtctgc gatgtgcatg aaacaacctc ctatcgagga gagaaggccg
     6781 ggtgatcgag cacacccgca aggtcgtcga cgatccagct cggggcgacg gcttcgaggt
     6841 cgctgcgcga ggtgcgtccc gtcgccaccg cgaggcacgg gatcccgtgc gcgcgcgcgc
     6901 acccgacgtc gagtaccgag tctccgatga ttaccgtctc gtcagggcga aagcgccgct
     6961 gggtcgcgag caacgcgcgc tcgagcgcga tcggcggcag gtcgttgcgg tcgaacacgt
     7021 tgtcgccgta actcccgaat ccgaagtagc gattgaggtc gtggcgcgag agcttgatgc
     7081 gcgcgccgcg ctcgaaattt ccggtcagga gcgccagcac gatatcgtcg cgcgcggcca
     7141 ggtgctcgag gagcggctcg atgccgggca gcacgcgcat gcgctcgcgc tcgaggctgc
     7201 ggtccagggt gtcgaggtat gcgtccttca ggcggggcaa cgtcgccagg atctcgtcgc
     7261 gcgggcgccc ggccatggtc atgaggtcga ccacgatgcg ggtgtcggtc ttgccggaga
     7321 agtcgtactc gtcgatggga ccggactcgc cgaagacggc gttcagcgcg gtcgcaaacg
     7381 cgacacgcgc ctgcccgccg caatcgacca aggttccgtc gatgtcgaat agaatgagct
     7441 tcacgggttt gcgtgtcgcg cgatccgttc gtagcttaga
//
LOCUS       DASPGK010000155         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_157807, whole genome shotgun sequence.
ACCESSION   DASPGK010000155 DASPGK010000000
VERSION     DASPGK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157807"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..411)
                     /locus_tag="VEC56_04475"
     CDS             complement(58..411)
                     /locus_tag="VEC56_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV13439.1"
                     /translation="MSKKLFVGSLSWNTTDEGLRRAFEKFGEVLEAKVIKDRETDRSR
                     GFGFVSFANPKDAMDAMAAMDGQELDGRTIKVNEAQERERTGGGPRGGGERRGGGGFG
                     GRGGGERRGGGGGRW"
     gene            complement(610..1101)
                     /locus_tag="VEC56_04480"
     CDS             complement(610..1101)
                     /locus_tag="VEC56_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV13440.1"
                     /translation="MHHRVSVPMLAACAAIVLATYAPALGEDAAKSKPAKTANKSNAQ
                     DAITVGPSIYRVILENDRVRVLEARFLPGASIGSHMHPDHVVVVVSAGKLSITNAEGT
                     REIDAKVGDTLFMPAETHSARNVGTTEFVCVVTELKGKYKTKGQASPPPGTVDVQQGK
                     VDG"
     gene            complement(1125..2549)
                     /locus_tag="VEC56_04485"
     CDS             complement(1125..2549)
                     /locus_tag="VEC56_04485"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXV13441.1"
                     /translation="MCGILGYCAREWDPGLVKFFGLQGFNLQHRGQENFGCTYSNGRK
                     FSHFKDRGLVSQVFTKERREDFTAARPLIAIAQTRYATAGGTDAISAQPHWMTLLDGF
                     YSNATNGDLPLLQDEIADLEKSGTVMLSGNDGEVILKKILHGAREHGGDLVAGIRRLM
                     ETTPGAYSGCLMTLTELYLYRDPHGFRPLYFGRWRDAVVFSSESCAFHERDATIEREV
                     LPGEVIRFDVFGKREHFQAVPTHAPRQQCIFEPIYFARPDSRVFGDEKEVGHFRYLLG
                     RRLATEHPLPDAAVVPVPDSGRYAAVGFASESGSPLRELLVRNPYIPRTFQIAGQANR
                     EDLVRLKFTVMHQLLRQYRRFLLVDDSLVRSTTLRILISMIRDAAEETGLARGEIEVH
                     VRVASPPIIGCCYYGIDTPEMDELVAAHQTIQEIAKGIDADSLEYLSLEGLDAVAREF
                     GNPKDFCHACFTGRYPTPFLPRSR"
     gene            complement(2595..3230)
                     /locus_tag="VEC56_04490"
     CDS             complement(2595..3230)
                     /locus_tag="VEC56_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV13442.1"
                     /translation="MDRQGPNYDPTQWLTLTEAAEALRVHPTTLRRWADSGQVPVFLT
                     PGGHRRFAESDVRHIAARRHSVRRVGPVERIWADQALSMARKQLAAQEGERWLKQHDG
                     GARDRNRQMGQQLMGLILEYLTAEDDDGPQLGQARKIGAHYGRFSRDLGLPLTDALRA
                     AMFFRDALTTSAIQLPDNVRIPTASQVRLVTRINRVVNEVQLGVADAYASS"
     gene            3357..3839
                     /locus_tag="VEC56_04495"
     CDS             3357..3839
                     /locus_tag="VEC56_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13443.1"
                     /translation="MKRLFYPLAIAGLAMTLAFLPACDDDDDNPMGGGPTTGTVAGTV
                     TFEGTWPSVGNVQISVYSTYPPMGPPDAFTDPIPAGTSYDYSIAGLDPGDYAAILVGW
                     RDPADPPGAKVIGMYWAFVDSVAVDGNGDPVGPPSAVTVEAGQTESNLDITANLDVAP
                     "
BASE COUNT          651 a         1277 c         1259 g          692 t
ORIGIN      
        1 aaacggcccg ggttcgcacc cgggccgttt cgctatgctt cgttgggaag cgtacgacta
       61 ccagcggccg ccgccgccgc cacgacgctc gccgccgccg cggccgccga agccaccgcc
      121 gccacgacgc tcgccgccgc cgcgcggtcc gccgccggtg cgctcgcgct cctgagcctc
      181 attgaccttg atggtgcggc catcgagctc ctggccgtcc atggcagcca tggcatccat
      241 ggcgtccttc ggattcgcga acgagacgaa accgaacccg cgggagcggt cggtctcgcg
      301 gtccttgatg accttggcct cgagcacttc gccgaacttc tcaaacgctc gccggagccc
      361 ctcgtcggtg gtgttccagc tcaagctacc cacaaagagt ttcttagaca cgatacattc
      421 tcctttgacg tttcccgcga tgcgataggc ttcccaatct gacaactaac taacaactcc
      481 gacgagaatc tccgcaccat cggtcaaaca ctgccagcaa gcataactca ttcccccggg
      541 ggggccaacc ctcatttttg ggctcttccg cgcaggttga cccggcagaa tccgccgggt
      601 tgcgattagt taaccatcga ctttcccctg ctgaacgtca accgttcccg gcggcggcga
      661 cgcctgcccc ttggtcttgt acttcccctt gagctcggtc acgacgcaca cgaactcggt
      721 ggtgccgacg ttacgggccg agtgggtctc cgccggcatg aacagggtat cgccgacttt
      781 ggcgtcgatc tcccgcgtcc cttcggcgtt ggtgatcgac agttttccgg ccgagacgac
      841 cacgacgacg tggtcggggt gcatgtgcga cccgatgctc gcgcccggca ggaagcgcgc
      901 ctccagcacg cgcacgcggt cgttttcgag gatgactcga tagatgctgg ggccgaccgt
      961 gatcgcgtcc tgcgcgttcg acttgttcgc cgtcttggcg ggcttgctct tcgcggcgtc
     1021 ctcgcccaag gcgggcgcgt acgtcgccag cacgatcgcc gcgcatgcgg cgagcatcgg
     1081 taccgatacg cgatgatgca tgacgaatcc tcctgggctt tctctcaacg cgaacggggc
     1141 agaaacggtg tggggtagcg gccggtgaag cacgcgtggc agaagtcctt cggatttccg
     1201 aactcgcgcg ccacggcgtc gaggccttcg agggaaagat actccagcga gtccgcgtcg
     1261 attcccttcg caatctcctg gatcgtttgg tgcgccgcca ccagctcgtc catctcgggc
     1321 gtgtcgatgc cgtaatagca gcacccgatg atggggggcg aagcgacccg cacgtgcact
     1381 tcgatctcgc cgcgcgcgag ccccgtctcc tcggcggcgt cacggatcat cgagatcagg
     1441 atgcgcagcg tggtcgatcg aaccagcgaa tcgtcgacca agaggaagcg gcggtactgg
     1501 cgcaggagct ggtgcatgac cgtgaacttc agccgcacga ggtcctcgcg gttggcttgc
     1561 cccgcgattt gaaacgtgcg cgggatgtac gggttgcgca ccagcaactc gcgcaacggc
     1621 gacccgcttt cgctcgcgaa gccgacggcc gcatagcgcc ccgaatccgg cacgggcacc
     1681 acggccgcgt ccgggagcgg gtgctcggtg gcgagacggc gccccaagag gtagcgaaaa
     1741 tgcccgactt ccttctcgtc gccgaacacg cggctgtcgg ggcgcgcgaa gtagatgggc
     1801 tcgaagatgc actgctggcg gggcgcatgg gtcggcacgg cctggaagtg ctcgcgcttg
     1861 ccgaatacgt cgaagcgaat cacctcgccg gggagtactt cgcgctcgat ggtggcatcg
     1921 cgctcgtgaa acgcgcacga ctccgacgag aagacgacgg cgtcgcgcca gcgcccgaag
     1981 tagagcgggc ggaagccgtg cgggtcgcgg tagaggtaca gctcggtcag cgtcatcagg
     2041 cacccggagt atgcacctgg tgtcgtctcc atcaggcgac gaattccggc caccaagtct
     2101 ccaccgtgct cgcgcgcgcc gtgcaggatc ttcttgagga tcacctcgcc gtcgttgccc
     2161 gagagcatca cggtgccgga cttctcgagg tccgcgatct cgtcttgaag caggggtaag
     2221 tcaccgttgg tcgcgttgga atagaagccg tcgaggagcg tcatccaatg gggctgggcc
     2281 gagatggcgt cggtgccgcc ggcggtggcg tagcgcgtct gcgcaattgc gatcagaggg
     2341 cgcgccgccg tgaagtcttc gcggcgctcc ttggtgaaaa cttgcgaaac gaggccccgg
     2401 tccttgaagt gggagaactt ccggccgttg ctgtaggtgc agccgaagtt ctcctgcccg
     2461 cggtgctgca ggttgaagcc ctgcaggccg aagaacttga ccaaccccgg gtcccactcc
     2521 cgggcgcagt atccgaggat tccgcacatg cgtcgatttc gatggagcgc cgcgaaattc
     2581 ggcggggcgt tatcttacga cgacgcgtag gcgtccgcta cacccaactg cacttcgttg
     2641 acgacccggt tgatgcgtgt gaccagccgc acctgcgacg cggtcgggat gcgaacgttg
     2701 tcggggagct ggatggcgct ggtcgtgagc gcgtcgcgga agaacatggc cgcgcgcagc
     2761 gcgtcggtga gcgggagacc gaggtcgcgc gaaaaccttc cgtagtgcgc gccgatcttg
     2821 cgcgcctgcc ccagctgcgg gccgtcgtcg tcctcggcgg tcaggtactc gagaatcagg
     2881 cccatcagtt gctgtcccat ctgacgattc cggtcgcgcg cccccccgtc gtgctgcttg
     2941 agccagcgct cgccctcctg ggcggcgagt tgcttgcgcg ccatcgaaag ggcttgatcg
     3001 gcccagatgc gttcgaccgg tcccacgcga cggaccgaat gccggcgcgc ggcgatgtgg
     3061 cggacgtcgg actccgcgaa gcgtcgatgc cctcccgggg tgaggaatac ggggacctga
     3121 ccggaatcgg cccaccgccg caaggtcgtc gggtgaacgc gcagcgcctc ggcggcctcg
     3181 gtcagagtca gccactgggt gggatcgtag ttggggcctt gacggtccat gaaagctgtg
     3241 tttgaatgct cgaaactatg caaaactaac cggtcgtgac gcaggttgca atgggccgga
     3301 cgccccgcgg cctgcgctcg acgggtcaca gaaaccttaa catcaggatt ggagccatga
     3361 aacgcctttt ctatccactt gcgatcgccg gcttggcgat gaccttggcc ttcctccccg
     3421 cgtgcgacga cgatgacgac aaccccatgg gtggcggccc gacaaccggc accgtggccg
     3481 gcacggtcac gttcgagggc acctggccct ccgttggcaa tgtgcagatc tcggtctact
     3541 cgacttatcc acccatggga ccgccggacg cgttcaccga cccgatcccg gcgggtacct
     3601 cgtacgacta ctcgattgca gggctcgacc ccggcgacta cgccgccatc ctcgtcggct
     3661 ggcgcgaccc ggccgatccc cccggtgcga aggtgatcgg aatgtattgg gcgttcgtgg
     3721 acagtgtcgc ggtcgacggc aacggcgatc cggtgggacc tccctccgcg gtgacggtcg
     3781 aagccggaca gacagagagc aatctcgaca tcaccgccaa tctggacgtg gcgccgtagg
     3841 actccgatcg tggtcgctct ccgcaccatc gtcgcgtgc
//
LOCUS       DASPGK010000156         8371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1095085, whole genome shotgun sequence.
ACCESSION   DASPGK010000156 DASPGK010000000
VERSION     DASPGK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095085"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VEC56_04500"
     CDS             complement(<1..1259)
                     /locus_tag="VEC56_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV13444.1"
                     /translation="MKQTIFSISKALGVALIAGIAIGAAARADQPGKVTPRLSEAMRA
                     AAPNETVRAWVFFADKAGAAKAEISDRARARRERNHVAVDQHDLPVTAAYVREVARTG
                     ARVRHASRWLNAVSVEADAADIDAIASLAFVRRIDRVRAYREPLPRYADVPQAVAPPR
                     QRATLALDYGPSLAQNVQINVPVVHGAGYSGSGVVIAVLDAGFNNMAHEALVDIDVLA
                     TWDFVNGDSIVSDQVGQAGTGNHGTYTLSALAGFEPGDLIGPAYGATFLLAKTENTDW
                     ERHVEEDDWIAGAEWADALGADVISSSLGYSTGFTPPDVDYSWEDMDGNTTIVAIGAD
                     IAASLGIVVVNSAGNGGFVSLPANTLGSPADGDNVLAIGAVDAAGIRAGFSSVGPSAD
                     GQIKPDVMAMGVGVISASPFSPTGYFGV"
     gene            complement(1357..2547)
                     /locus_tag="VEC56_04505"
     CDS             complement(1357..2547)
                     /locus_tag="VEC56_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13445.1"
                     /translation="MPTMSSQPPRSSKRPSPSRRILAYGALLAFAGLAALGIAEVILR
                     LVPIPGITYHSFYFDPLTGGKHYPNSTLIYQRAGTTVVRRANSWGYPDVDHELEPAPG
                     TLRIGFFGDSYTEAIQVPLEDTFFRRIEESLNARIPDLVGGSNRRGEPVRHIETISFG
                     VSGRSTLQSYLECTQWMEKSDLDVVVYVFVENDPGDQLAGVQPSDEIPLAVVAGDSFA
                     VDNSFLQRYGHKTTWWHRAIQRVKANSLVVSTVEGRLKLLKRHGIKRAVTEADRAGGA
                     QGGGVTMVPSAWPPERAEEGWMLVERVLDRWRREVAAQGRTFVIFRVPREEVVAVPLP
                     EQDAWAPRLHEYCARRGIPLVDPTPYFVPRMASGDAVYGDHFTALGHRLFADAFVDYW
                     LQRG"
     gene            complement(2603..3286)
                     /locus_tag="VEC56_04510"
     CDS             complement(2603..3286)
                     /locus_tag="VEC56_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13446.1"
                     /translation="MEQLTLFIAAIVLALGIALRVFAQKFGAMAKPPLRIQLVPVDDR
                     AKGRFADPHRRELESLGFTPMGTYQVREMPGVTLVAFTQSFQAVCAVVYRHPLAGVFI
                     DMVSATEDDRGLTVTNAPAGGNLDQPPGRAKIFEPKARLRRLYDRLVSERPAGPYQRI
                     DASNFMRVFEAEYAREMEWRQKRGGPTEEEVRREAHAMGIRNEKMIQKATQNLRKQYS
                     EPDQPTGVR"
     gene            complement(3348..6479)
                     /locus_tag="VEC56_04515"
     CDS             complement(3348..6479)
                     /locus_tag="VEC56_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HXV13447.1"
                     /translation="MLRGFGMRNYAVAGLWGILAAGPVLAGTESRSDIDRAIDVYLAE
                     GQTDSAAALAERALDLATAQFGGDARAVAVYADSLGNRFFQAYDFAHGVALFERGVTL
                     RESVLAPDDLALADPIENLSTAYYIAGRYDEAAIAQERVVAIKAAKLGPDDASTARSR
                     LDLAIVYFPSARYADAERELRAAIRSLEPARGEDPLPLAQAEQVLGEVCRELNRYREA
                     EEYLTDALALVRRGLPERDPGTFTYLNSLAGYYKDQARYDESELLLEEALDIVEAHPS
                     LEDERAALTLNMAEVHRLQGRYDDAIPLYQSAIALATKSMPSLDVAIFRNQIASAYAE
                     MGRPRDAEAQYRQALAVADSSTDASPLVIAQFKNDLGVLLAREGRLADGESLLGEAIT
                     LRESVYGPSHPLVAVSLTQLARAQARLFGGASSKRTPRDAEAALLLDRALSIFDSTTA
                     EPEGRVDAGISRAELYHRDHKIDRAAETMAGALENVESLRPYRGGGGEERIEFMRRYV
                     DAYDRMTAWQVELGKVGPALDYSERRRARVLVDQLTGASRGAPTEEARAALERLRVDR
                     RDLESQLGKCQADAGALRAKPRLSKSERSQLASIEATCDRIVGEIQRAGERIRRLEST
                     GNYSPAPTAAARPRVPTGEALLVYHVGGESSFVFIVEGEAVRAYPLKVSSEAFAKLGM
                     PSGPLTRASLAQLLSGYDAAGKPAGLGILRQLATPVESSAQADVAARAHASALDRLHA
                     LFGVLMPAEVWSRIRTARQVTVIPDGPLASFPFEALVVTREKNDASFWLDDGPVVRYA
                     PSIATLEALASAGRARTASRDVLSVCNPRYATANEGTVTKPVTRGGPLSPLPGTERET
                     ETVVAAFGKDRVTVLCGEAATEAAVRRAVAGKEIVHLATHGLVSERRSDLLTALAFTP
                     ASSPPRDLRDDGFLHLFEIYDLALSAELVVLSACESSTGSYVLGEGVMALSRGFLSAG
                     ARRVVATQWKVDDDATAALVGEFLSRVASAGRANQSVDYARALREAKRTVRARAEWSQ
                     PFYWAAFVVSGAP"
     gene            6589..7320
                     /locus_tag="VEC56_04520"
     CDS             6589..7320
                     /locus_tag="VEC56_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13448.1"
                     /translation="MHRRLLSRVLIAVAAFASIGASASEVLAQTQQLNKELVRKFPEG
                     FCPLDTNFIGTFVSNTGIIQLTFVTGLGPHGNGLKSGDVAVGFNQVLDDVAIIEAAEF
                     AANSVENSNFCYSDGNPFVPEYPVGLLAGNPKVPFFDLFNPVTSPCPWDETNGATINL
                     VDGWIEFTGAGDVASTSVVISGLTPGTQYVIAGQWSAIEFPFATECTPTSLCLQITVD
                     DLPNGCGPLPVETKTWGQVKALYNK"
     gene            complement(7330..7635)
                     /locus_tag="VEC56_04525"
     CDS             complement(7330..7635)
                     /locus_tag="VEC56_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXV13449.1"
                     /translation="MTAYERIYRVVRRIPRGRVATYGQVAELAQASGPRQVGYALHAL
                     PDGSDVPWHRVVNAQGRISLRSGLGGESFQNALLKAEAVEFDAKGRIDLDRFRWKKR"
     gene            complement(7652..>8371)
                     /locus_tag="VEC56_04530"
     CDS             complement(7652..>8371)
                     /locus_tag="VEC56_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13450.1"
                     /translation="GQYVELFNTTAADIVLPNIYFLCSHFVYAQVAGTVPAGGYATVP
                     WPASFTTAVDASGEMMLYDSGLFGTSTDILDYVIWGNPAITRKNQALAVGKWVGANAP
                     SISGGAIHRRIGVQGNEAADYDAAAAPTPENCTPDPGTGVGDTPALTGARIWNSPNPF
                     KSSTNVDFVLDAPASVEVAVYAIDGSLVKVLATQSYAAGTHRATWDGTDQAGRTVASG
                     TYLARVSGHGVSAAARLTVIR"
BASE COUNT         1364 a         2819 c         2826 g         1362 t
ORIGIN      
        1 acgccgaagt atccggtcgg gctgaacgga ctggccgaaa tcacgcccac gcccatcgcc
       61 atcacgtcgg gcttgatctg cccgtccgcc gatggaccga cggagctgaa accggcgcga
      121 atgccggccg cgtccacggc accgatcgcg agcacgttgt cgccgtccgc gggactcccg
      181 agcgtgttgg ccggcagcga cacgaagccc ccgttgccgg cggagttcac gaccacgatg
      241 ccgaggctgg cggcgatgtc cgcgccgatc gcgacgatgg tcgtgtttcc gtccatgtct
      301 tcccacgagt agtcgacgtc gggcggagtg aatccggtgg agtaaccgag cgagctcgag
      361 atgacgtcgg cgccgagcgc gtcggcccac tccgcgcccg cgatccagtc gtcctcttcg
      421 acgtgacgct cccagtcggt gttctcggtc ttggcgagca ggaacgtggc gccgtacgcg
      481 ggaccgatca agtcgcccgg ctcgaagccg gccagcgcac tcaacgtgta ggtgccgtgg
      541 ttgccggttc ccgcctgccc cacttggtcg gaaacgatgc tgtcgccgtt gacgaaatcc
      601 cacgtcgcga ggacgtcgat gtcgacgagg gcttcgtgcg ccatgttgtt gaagcccgcg
      661 tcgagcaccg cgatcaccac cccgctcccg ctgtagcccg cgccgtgcac caccggcacg
      721 ttgatctgca cgttctgcgc gagcgagggg ccgtagtcga gggccagcgt ggcgcgctgg
      781 cgcgggggcg cgacggcctg cggcacatct gcgtaccttg gaagcggctc gcgatacgcg
      841 cgcacgcggt cgatgcggcg cacgaaggcg agcgacgcga tggcgtcgat gtcggcggca
      901 tcggcctcga ccgaaaccgc gttcagccag cgcgacgcgt gccgcacgcg ggcgccggtg
      961 cgcgcgacct cgcgcacgta ggccgccgtg accgggaggt cgtgctggtc cacggcgaca
     1021 tggttgcgct cgcgccgagc gcgcgcgcgg tcggagatct cggccttggc ggcgcccgcc
     1081 ttgtcggcga agaacaccca cgcccgcacc gtctcgttgg gcgcggctgc gcgcatcgcc
     1141 tcggacaatc gcggcgtcac cttccccggc tggtccgcac gtgcggcggc gccgatcgcg
     1201 atccccgcga tcagcgcgac accgagggct ttggaaattg agaagatggt ttgcttcaac
     1261 gggtggactc ccacgcgcgc cggcagagcg ccccggcgga ggcatttatt ataaccgaaa
     1321 cgtctcggcg aacggaaggc gcgcgtcgct tcaaagttag ccgcgctgga gccagtagtc
     1381 cacgaacgcg tcggcgaaca gacgatgccc cagcgcggtg aagtggtcgc catagaccgc
     1441 atccccggac gccatgcgcg ggacgaagta cggcgtggga tcgacgagcg ggatgccgcg
     1501 gcgggcgcag tactcgtgca ggcgcggcgc ccacgcgtct tgttccggca gcggcaccgc
     1561 gacgacttcc tcgcgcggta cgcggaaaat gacgaacgtc cggccctggg ccgcgacctc
     1621 gcgccgccag cggtcgagca cgcgttcgac cagcatccag ccctcctcag cgcgctccgg
     1681 cggccacgcc gacggcacca tggtgacgcc cccaccctgg gcgccgcctg cgcgatctgc
     1741 ctccgtcacc gctcgtttga ttccatgacg cttgaggagc ttcaagcgac cctcgaccgt
     1801 gctcacaacc aatgagttgg ccttgacacg ctgtatcgca cgatgccacc acgtggtctt
     1861 gtgtccgtaa cgctgcaaga acgagttgtc gacggcgaac gaatcgcccg cgacgacggc
     1921 gagcggtatc tcatcggagg gctgaactcc ggccagctga tcgccggggt cgttctcgac
     1981 gaagacgtac accaccacgt cgaggtcgct tttttccatc cactgcgtgc attcgagata
     2041 cgactgcagc gtgctgcgtc ccgacacgcc gaacgagatg gtctcgatgt ggcgaaccgg
     2101 ctccccgcgc cggttgctcc cgcccacgag atccgggatg cgggcgttga gcgattcctc
     2161 gattcgtcga aagaacgtgt cctccaacgg gacctgtatg gcctcggtgt aggagtcgcc
     2221 gaagaacccg atgcgcagcg ttcccggagc gggttcgagt tcgtgatcga cgtcggggta
     2281 tccccacgag ttcgcgcggc gaacgacggt ggtgccggca cgctgataga tgagcgtcga
     2341 gttcgggtag tgcttcccgc ccgttagcgg atcgaaatag aacgagtggt acgtgatccc
     2401 gggaatcggg accaggcgca ggatgacttc ggcgatgccg agcgcagcca gtcccgcgaa
     2461 tgcgagcagc gcgccgtagg cgagaattcg tcgcgatggc gacggacgct tcgatgaacg
     2521 cggcggctgc gagctcatgg tgggcaagac gaacccgcgg atcttacccg ctcgtgcgcc
     2581 tcgcgtccac tgctgatgcg cgttagcgga cgccggtggg ctggtcgggc tcgctatact
     2641 gcttgcgcag attttgggtg gccttctgga tcatcttctc gttgcggatg cccatggcgt
     2701 gcgcttcgcg gcggacttcc tcttcggtcg gaccgccgcg tttctgtctc cactccatct
     2761 cgcgcgcgta ttcggcctcg aagacgcgca tgaagttcga cgcgtcgatg cgctggtacg
     2821 gtcccgcggg gcgctccgag accaagcgat cgtagaggcg ccgcagccgg gccttgggtt
     2881 caaaaatctt cgcgcgcccg ggcggctgat cgaggttgcc gccggcgggg gcgttggtca
     2941 ccgtcaaccc gcggtcgtct tcggtcgcgc tgaccatgtc gatgaatacg cctgcgagcg
     3001 ggtggcgata gacgaccgcg cacacggcct ggaacgactg cgtgaacgcg accaaagtca
     3061 cgccgggcat ctcgcgcact tggtaggtcc ccatcggcgt gaacccgaga gattccaact
     3121 cgcgacggtg tggatcggca aagcgcccct tcgcacgatc gtcgaccgga acgagttgga
     3181 tacgtagcgg cggcttggcc atggcgccga acttttgggc gaagacccgg agggcgatgc
     3241 cgagcgccag cacaatggct gcgataaaga gggtgagttg ttccacgatc gacctccgat
     3301 tgccgttcgg gaggtcgatg tgcaagggcg atgccagggc gggggttcta aggagctccg
     3361 gagaccacga aggcggccca gtagaagggc tgggaccatt cggcgcgcgc gcggaccgtg
     3421 cgcttggcct cgcgcagtgc acgcgcgtag tcgacggatt ggttcgcgcg cccggcggag
     3481 gcaacgcggg acaggaactc gccgaccagc gccgcggtgg cgtcgtcgtc gaccttccac
     3541 tgcgtcgcta ccacacgccg cgctcccgcc gagagaaagc ctcgcgacag cgccatgact
     3601 ccctcgccca gcacgtagct gccggtgctg ctctcgcacg cgctcaacac gaccaactcg
     3661 gcggagagcg cgaggtcgta gatctcgaac aggtgcagga acccgtcgtc gcgcaggtcg
     3721 cgcggcggcg acgacgccgg ggtgaacgcc agcgcggtga gcaggtcgct ccttcgttcg
     3781 ctcaccagcc cgtgggtcgc caggtgcacg atctcctttc cggcgaccgc gcggcggacc
     3841 gccgcttcgg tggccgcctc gccgcacagg accgtcacgc gatccttgcc gaaggcggcc
     3901 acgaccgtct cggtctcgcg ctcggtaccg gggagcggcg agagcggacc gccgcgtgtc
     3961 accggcttgg tgacggtgcc ctcgttcgcc gttgcgtaac gaggattgca gacactcaac
     4021 acgtcgcgcg acgccgtgcg ggcgcgcccg gcggacgcca gcgcctccag cgtcgcgatc
     4081 gagggcgcgt agcgaaccac gggcccgtcg tcgagccaga aggacgcgtc gttcttctct
     4141 ctcgtgacca cgagcgcttc gaacggaaac gatgccagcg gcccatcggg gatcacggtg
     4201 acttgccgcg cggtccgaat ccgcgaccac acctcggccg gcatcagcac gccgaagagc
     4261 gcgtgcaggc gatcgagcgc gctcgcgtgc gcgcgcgcgg cgacatccgc ctgcgcgctg
     4321 gactccacgg gagtcgcgag ttggcgaagg atgcccaggc ccgcgggctt ccccgccgcg
     4381 tcgtagccgg agaggagttg ggcgagcgac gcgcgcgtga gcgggccgga gggcatgccg
     4441 agcttggcga aggcctcgct cgacaccttc agcgggtacg cacgaaccgc ctcgccttcg
     4501 actatgaaca cgaagctcga ttcgccgccg acgtgataaa cgagcagcgc ctcgcccgtg
     4561 ggaacgcgcg ggcgcgccgc ggcagtcggg gccggcgagt agttgcccgt gctctcgagg
     4621 cggcggatgc gctcgccggc gcgctggatc tcgcccacga tgcgatcgca cgtcgcttcg
     4681 atggaggcca gctgggagcg ctccgacttc gagaggcgcg gcttggcgcg aagcgcgccc
     4741 gcatccgctt ggcacttgcc cagctgcgac tccagatcgc gacgatcgac gcgcaggcgc
     4801 tccagcgccg cgcgtgcctc ctcggtggga gcaccacgcg aggcacccgt cagctggtcc
     4861 accagcacac gcgcgcgacg ccgctccgaa taatcgagag ccgggccgac ctttcccagc
     4921 tcgacctgcc acgccgtcat gcggtcgtag gcgtcgacgt agcgtcgcat gaactcgatg
     4981 cgctcctcgc caccgccgcc gcgatagggt cgaagcgatt ccacgttttc gagggcaccg
     5041 gccatggtct cggccgcgcg gtcgatcttg tgatcgcgat ggtaaagctc cgcacgcgat
     5101 atgccggcgt cgacgcgacc ctcgggctcg gccgtggtgg aatcgaagat cgagagcgcg
     5161 cgatcgagca agagggccgc ttcggcgtcg cgcggggttc gcttgctcga tgcgccccca
     5221 aagagtctcg cctgcgcgcg cgccagctgc gtcagcgaca ccgcgaccag ggggtggctc
     5281 gggccgtaga cgctctcacg gagcgtgatg gcttccccga gcagcgactc gccatcggcg
     5341 agtcggcctt cgcgggcgag cagaaccccc aggtcgttct tgaactgcgc gatcaccagc
     5401 ggcgacgcat ccgtggaaga atcggcgacg gcgagcgcct gccggtactg tgcctccgcg
     5461 tcccggggtc ttcccatttc cgcatacgcc gatgcaatct gattgcgaaa gatggccacg
     5521 tcgagcgacg gcatggactt ggtggccagc gcgatcgcac tctggtagag cggaatggcg
     5581 tcgtcgtagc ggccttgcag gcgatgaacc tcggccatgt tgagcgtgag ggcggcacgc
     5641 tcatcctcga gacttgggtg cgcctcgacg atgtcgagcg cttcctccag gaggagctcc
     5701 gattcgtcgt agcgcgcttg gtctttgtag tacccggcca ggctattcag gtaggtgaag
     5761 gtccccggat cgcgttcggg aaggccccta cgcacgagtg ccaacgcatc ggtgaggtac
     5821 tcctccgcct cgcggtaccg attaagctcg cgacacactt cgcccagaac ttgctcggct
     5881 tgggcaaggg gaagcgggtc ttccccgcgc gccggttcca gcgaccggat ggcggcgcgc
     5941 agctcgcgct cggcgtcggc gtagcgcgcg ctcgggaagt agacaattgc caggtcgagg
     6001 cggcttcgtg cggtggacgc atcgtcgggc ccgagcttcg cggccttgat cgccacgacg
     6061 cgctcttggg cgatggcggc ctcgtcgtag cggccggcga tgtagtacgc ggtggacaga
     6121 ttctcgatcg ggtcggcgag tgcgaggtcg tcgggggcca gcaccgattc gcgaagcgtg
     6181 accccgcgct cgaacagcgc cacgccgtgg gcgaaatcgt acgcttgaaa gaagcgattc
     6241 ccgagggagt ccgcgtaaac ggcgaccgcc cgcgcatcgc caccgaactg ggcggtcgcg
     6301 agatcgagcg cgcgctcggc cagcgccgcg gcgctatcgg tctgcccctc cgcgaggtag
     6361 acatcgatgg cccggtcaat gtccgaccgg gactccgtcc ccgccaagac ggggccggcc
     6421 gccaggatcc cccataaccc ggcgaccgcg taattccgca taccaaagcc acggagcatg
     6481 ccgctgacct cccaaaaagg cacgggggca tgatatactg tcccgcgtat tcgcctggat
     6541 gcatttctgc gagcgccagt cacaacccgt ttcgaaccgg agggccccat gcaccgaaga
     6601 ctgctatcgc gcgtccttat cgccgtcgcg gcgttcgcct cgatcggcgc gtccgcgagc
     6661 gaggtactcg ctcagaccca gcaactcaac aaggagctgg tccgcaagtt cccggagggc
     6721 ttctgcccgc tggatacgaa cttcatcggc acgttcgtct ccaataccgg gatcattcaa
     6781 ctgacgttcg tcaccgggct tggcccccac ggcaacggtt tgaaatcggg agacgtcgcg
     6841 gtcggcttca accaggtgct ggacgatgtc gccatcatcg aggcggcgga gttcgcggcc
     6901 aactccgtgg agaacagcaa cttttgctac tccgacggga acccgttcgt gcccgagtac
     6961 cccgtcggcc ttctggctgg gaacccgaag gttcccttct tcgatctctt caacccggtg
     7021 acgagcccgt gcccgtggga cgagaccaac ggcgccacca tcaatttggt cgacggttgg
     7081 atcgagttca ccggcgccgg tgacgtcgca tcgacgagtg tggtgatttc cggtttgacg
     7141 ccgggaacgc aatacgtcat cgccggccag tggtccgcga tcgaattccc gttcgcaacc
     7201 gagtgcacac cgacgtcctt gtgcctgcag atcaccgtcg acgacttgcc gaacggttgc
     7261 ggcccgctgc cggtcgaaac caagacctgg ggtcaggtga aggcgctcta caacaagtag
     7321 aacgaaacgc tagcgcttct tccaacgaaa gcggtcgagg tcgatgcgtc ccttcgcgtc
     7381 gaactcgacc gcttccgctt tcaggagggc attctggaag gactcgccgc ccagacccga
     7441 gcgcaggctg atgcgcccct gagcgttcac gacgcggtgc caggggacgt cgcttccgtc
     7501 gggcagcgcg tggagcgcgt atccgacttg ccgtggcccc gacgcctgcg caagctccgc
     7561 gacctggccg taggtcgcga cacgaccccg cgggatccgt cgcacgacgc ggtagatgcg
     7621 ctcgtacgcg gtcacgcgct cccggtccgt cctagcgaat gacggtcaga cgcgcggctg
     7681 cggaaacgcc atggccggag acgcgcgcga gatacgtgcc gctcgcgacc gtccgcccag
     7741 cctgatcggt gccgtcccag gttgcgcgat gcgttccggc cgcatacgac tgcgtcgcca
     7801 gtaccttcac caacgacccg tcgatcgcgt acaccgccac ttccaccgac gcgggggcgt
     7861 cgagcacgaa gtcgacgttg gtgctcgact tgaacggatt ggggctgttc cagatgcgcg
     7921 ccccggtcag cgcgggcgtg tcgccgactc cggtccccgg atcgggcgta cagttctccg
     7981 gcgtcggagc cgcggcggca tcgtagtccg cggcttcgtt cccctggacg ccgatgcggc
     8041 gatggatggc gccaccgctg atcgagggcg cgttggcgcc gacccatttg ccgaccgcga
     8101 gcgcttggtt cttgcgcgtg atcgcggggt tgccccagat gacgtagtcg aggatgtcgg
     8161 tgctggtacc gaacaacccg ctatcgtaaa gcatcatttc gccgctggcg tcgaccgcgg
     8221 tcgtgaagct cgccggccag ggcacggtcg cgtatccacc cgccggtacc gtgcccgcca
     8281 cctgcgcgta cacgaaatgc gaacacagga agtagatgtt cggcaacacg atgtcggccg
     8341 cggtggtgtt gaacaactcg acgtactgcc c
//
LOCUS       DASPGK010000157         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_977396, whole genome shotgun sequence.
ACCESSION   DASPGK010000157 DASPGK010000000
VERSION     DASPGK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977396"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..890
                     /locus_tag="VEC56_04535"
     CDS             165..890
                     /locus_tag="VEC56_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV13451.1"
                     /translation="MIRIQHLNKSFDALAVLNDVSLEIQRGAITAILGPNASGKSTLI
                     KCIVGLVNPDSGKILVDDDPITGRWHYRSKIGYMPQIARFPENLTVDELFAMLRDLRE
                     NDSDVDIELFARYELGTMGRKRLGQLSGGTRQKILAAIAFLFAPKILILDEPTVGLDP
                     LATAAFKDKLRKERAAGRTILLSSHLVNEVEELADNIVYLQDGRPFFDGTIDEIRRAT
                     AEARLDRALVRVMEGEVGHGTRR"
     gene            874..1665
                     /locus_tag="VEC56_04540"
     CDS             874..1665
                     /locus_tag="VEC56_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXV13452.1"
                     /translation="MGHAGKILRYEFQNMLRSKWLIGIAVMLLAVTEALFRFGGDPAK
                     TIASVMNVVLVVVPLLSVVLGTIYFYNSREFNELLLSQPVNRTSIYLGKLVGFAAALC
                     LAYAVGIGLPFLIHSFGLREYAGKIATLLLVGCCFIVIFSSLAFYAATRFEDRIKGLG
                     ATLLAWFFLAVIWDGVILLFVHLFREYPYEPGLLAMVFANPIDLGRILILMQLDISAL
                     MGYTGAVFRSVFGAGQGTLLAAAAMVIYAAVPVVFGLRMFRAKDF"
     gene            1740..2141
                     /gene="mscL"
                     /locus_tag="VEC56_04545"
     CDS             1740..2141
                     /gene="mscL"
                     /locus_tag="VEC56_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXV13453.1"
                     /translation="MSMIKEFRDFAVKGNMIDMAVGIIIGAAFGKLITSLVNDIIMPP
                     LGQLIGGVNFTELKLTIGSPIEGREPATLNYGSFIQTAFDFLIVAFAIFMVVKTINKM
                     KRKQEAAPAAPPAPTKDQVLLTEIRDLLKAR"
     gene            complement(2138..3211)
                     /locus_tag="VEC56_04550"
     CDS             complement(2138..3211)
                     /locus_tag="VEC56_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV13454.1"
                     /translation="MKRVLACLMLSLVTASLLPTTNATAQKKSNYQFSDSHFHLTNYI
                     QEGISAKQFLELMGNRVERSTLFGIPLQQQWSYRVSGDDAPTYYLDTDADLYYYSFTD
                     AWIAQQCLALSKKDRARFDPMITGFNPADMYAADHIRRVLLTYPGVFTGIGEFTIHKE
                     FVSSKIAGDVASLTDPALDRIFDFVAETGMVAILHNDIDRPIASKSAEPVFLSQLKDL
                     FRRHPKATIVWAHVGVGRIVHPVENHLGMLESMLSDPAYAHVYFDLSWDETAKYVVSS
                     PEATRRTADLLNRYPDRFMFGTDTVAPGSPEKYFAVYEMYQPLWDSLTQEASENVRLG
                     NYRRIFDEGRRRVRAWEKANLKP"
     gene            complement(3221..>4466)
                     /locus_tag="VEC56_04555"
     CDS             complement(3221..>4466)
                     /locus_tag="VEC56_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13455.1"
                     /translation="AQDEAAIPDTTAAAPEDIHPNTIAGEFSPAKGFDIIKTKRGSLN
                     ISAYGLFRFLNQTPAEQTFRDHLGRERQVVARNDINWHRTMIWVSGFFYDPKFRYTIT
                     VWSLPTTEQTLVFGLLRYLASKPLTVAAGIGPSLTARSMQGSWPFWAGSDRTMAEEFY
                     RGGFATSFFVTGEPLDRFFYTASINRSLSQLGIPAGNDNRSYAYSASLLWMPTTGEFG
                     PRGGFGDLEHHEQVATRFGISACHAREGRYAPDTQSPRHAQLKLSDGVNPFETGALAD
                     TVTVNELDYDEIAFDAGFKYRGFSFQAEYGLRVLSDFDADGPLPDDSIFDHGFYAQAM
                     HMVVPKKLGIYATTGLIFDEFGRDPWEVSGGASFYPYGSRSLRLNLHYIHVEKSPTGS
                     NFGYYTAGQTGDTISMGVDILL"
BASE COUNT          792 a         1381 c         1430 g          863 t
ORIGIN      
        1 ccgccgggca tcgtgctctt gaacagcctg tttgttacgt tgatcgacac tgccgagcga
       61 gtgatgcccg tgttcacgcc cgagacgctg gtggacgcga gcccgctcat gagcgtggtc
      121 gtcgtgcggc cgaccggggc cccggcgccg caggagggat cccgatgatt cgcatccagc
      181 acctcaacaa gtcgttcgac gccctcgcgg ttctcaatga cgtgtcgctc gagatccagc
      241 ggggcgcgat caccgccatc ctgggcccca acgcgtcggg gaaatcgacg ctgatcaagt
      301 gcatcgtcgg gcttgtcaac cccgactcgg gcaagatcct ggtcgacgac gatcccatca
      361 cggggcgctg gcactatcgt tcgaagatcg gctacatgcc gcagatcgcg cgcttccccg
      421 agaacctcac ggtcgacgag ctgttcgcga tgctgcgcga cttgcgcgag aacgacagcg
      481 acgtcgacat cgaactcttc gcgcgctacg aactggggac catggggcgc aagcgtctgg
      541 gccagctctc gggcgggacg cggcagaaga tcctggccgc catcgcattc ctgttcgcgc
      601 ccaagatcct gatcctcgac gagcccaccg tcgggctcga cccgctcgcg accgcggcct
      661 tcaaggacaa gctccgcaag gagcgcgcgg ccgggcgcac gatccttttg tcgtcgcacc
      721 tggtgaacga agtcgaggag ctggccgata acatcgtcta tctgcaggac ggacgcccgt
      781 tcttcgacgg caccatcgat gagatcaggc gcgccaccgc cgaagcgcgg ctcgaccgcg
      841 cgctcgtgcg cgtaatggaa ggggaggtcg gtcatgggac acgccggtaa gatccttcgc
      901 tacgaattcc agaacatgct gcgcagcaag tggctgatcg ggatcgcagt catgctgctc
      961 gcggtcaccg aggcgctgtt ccgcttcggc ggcgaccccg ccaagaccat cgccagcgtc
     1021 atgaacgtcg tcctggtggt ggtcccgctg ctctcggtcg tgctaggaac gatctacttc
     1081 tacaactcgc gcgagttcaa cgagctgttg ctttcgcagc ccgtcaatcg gaccagcatc
     1141 tatctcggaa agctggtggg tttcgccgcc gcgttgtgcc tcgcctacgc ggtggggatc
     1201 gggctgccgt tcctcattca ctcgttcggg ctgcgggagt acgcgggaaa aatcgcaacg
     1261 ctgctcctcg tcggctgctg cttcatcgtc atcttctcgt cgctcgcgtt ctacgcagcc
     1321 acgcgcttcg aggaccgcat caaagggctg ggcgcgacgc tattggcgtg gttcttcctc
     1381 gccgtgatct gggacggcgt gatcctgctg ttcgtgcacc tgttccgcga gtacccctac
     1441 gagcccggat tgctcgcgat ggtgttcgcc aacccgatcg acctcggccg catcctgatc
     1501 ctgatgcaac tcgacatctc ggcgctcatg ggctacacgg gagcggtgtt ccgcagcgtg
     1561 ttcggggccg gccaggggac gttgctcgcg gccgcggcca tggtgatcta cgccgcggtg
     1621 ccggtcgtgt tcggcctgcg gatgttccgc gcgaaggact tctaggtcaa tttcgagctt
     1681 gaatcgacgg cgtgcccgtc ggtaagttcg ggccgttcgt tcaccaagga ggacgactcg
     1741 tgagcatgat caaggagttc cgcgatttcg ccgtcaaggg caacatgatc gacatggcgg
     1801 tgggcatcat catcggcgcg gctttcggaa aactcatcac ctcgctcgtc aacgacatca
     1861 tcatgccgcc cttggggcag ctgatcggtg gggtcaattt cacggagttg aagctgacca
     1921 ttgggtcacc catcgaaggc agggagcccg cgacgctcaa ctatggcagt ttcatccaaa
     1981 ccgcgtttga ttttctgatc gtcgcgttcg ctatcttcat ggtcgtcaag accatcaaca
     2041 agatgaagcg caaacaggag gcggcaccgg cggcccctcc ggcgccgacc aaggaccagg
     2101 tgctcctgac cgaaattcgc gacctgctga aggcgcgcta gggcttgaga ttcgctttct
     2161 cccaggcgcg aacgcgacgg cggccctcgt caaagatgcg ccgatagttg cccaggcgca
     2221 cgttctcgct ggcttcttgc gtgaggctgt cccacagcgg ctggtacatc tcgtacaccg
     2281 cgaagtattt ctccggcgag ccgggcgcta ccgtatccgt cccgaacatg aagcggtcgg
     2341 ggtagcggtt caggagatcg gccgtgcgtc gcgttgcctc cggcgaggag accacgtatt
     2401 tcgccgtctc gtcccacgat aggtcgaagt acacgtgggc gtaggccgga tccgacagca
     2461 tgctctcgag cattcccagg tgattctcca ccgggtggac gatgcgcccc acgccgacgt
     2521 gggcccacac gatcgtcgct ttgggatgtc gacggaagag gtccttgagt tgggacagga
     2581 acaccggctc cgcgctcttc gatgcgatgg gccggtcgat gtcgttgtgg aggatcgcga
     2641 ccatcccggt ctccgcgacg aagtcgaaga tgcggtcgag cgccgggtcg gtgagactcg
     2701 ccacgtctcc cgcgatcttc gacgacacga actccttgtg gatcgtgaac tcaccaatgc
     2761 ccgtgaacac gcccggatag gtgagcagca cacggcggat gtggtcagcc gcgtacatgt
     2821 cggcggggtt gaacccggtg atcatggggt cgaagcgcgc ccggtccttc ttggacagcg
     2881 ccaggcactg ctgcgcgatc cacgcgtcgg taaacgagta gtagtagagg tcggcgtcgg
     2941 tatccaggta gtacgtgggc gcgtcgtcgc ccgagacccg gtacgaccac tgctgctgga
     3001 gcgggatgcc gaacagcgtc gagcgctcca cgcgattgcc catcagctcg aggaactgct
     3061 tcgccgagat gccttcctgg atgtagttgg taagatggaa gtgcgagtcg ctgaactggt
     3121 agttcgattt cttttgggcc gtcgcgttcg tcgttggcaa cagcgacgcc gttaccaagg
     3181 aaagcatcaa acaagcgagc acgcgtttca tcgattcctc ctataacagg atgtccactc
     3241 ccatggaaat cgtgtctccg gtttgcccgg cggtatagta tccgaagttc gaccccgtcg
     3301 gcgacttctc gacatggatg tagtgcaaat tgaggcgcag gctccggctc ccgtaggggt
     3361 agaagctcgc gccgccggag acttcccatg gatcgcgtcc gaactcgtcg aagatgaggc
     3421 cggtggttgc gtagatcccg agcttcttcg gcaccaccat gtgcatcgct tgcgcgtaga
     3481 aaccatggtc gaagatagag tcgtcgggaa gcggaccgtc ggcgtcgaag tcggacagca
     3541 cgcgtaaacc gtactcggcc tggaacgaga aaccccggta cttgaatccc gcgtcgaacg
     3601 cgatctcatc gtagtcgagc tcgttgacgg tgacggtgtc ggcgagggct ccggtctcga
     3661 acggattgac gccgtccgaa agcttgagct gcgcgtggcg cggtgactgg gtatcgggcg
     3721 cataacggcc ctcgcgcgcg tggcacgcgg agatcccgaa gcgtgtcgcc acctgctcgt
     3781 ggtgctcgag atcgccgaag ccgccgcgcg gtccgaactc gccggtcgtc ggcatccaga
     3841 gcaggctggc gctgtacgcg tacgagcggt tgtcgttgcc cgcgggaatg ccaagctgac
     3901 tcaagctacg attgatcgat gccgtgtaga agaagcgatc cagcggctcg ccggtgacga
     3961 aaaacgacgt cgcgaagccg ccgcggtaga actcctccgc catggtgcgg tcgctgcccg
     4021 cccagaacgg ccacgacccc tgcatcgagc gcgcggtcaa gctgggcccg atccccgcgg
     4081 ccaccgtcag cggttttgac gcgaggtagc gcagcaatcc gaacaccagt gtctgctcgg
     4141 tagtggggag cgaccacacc gtgatcgtgt accggaattt cggatcgtag aagaacccgc
     4201 tcacccagat catggtgcgg tgccagttga tgtcgttgcg cgcgaccacc tgtcgttcgc
     4261 gccccaagtg atcgcgaaac gtctgctcgg cgggagtctg attcaggaag cggaacaatc
     4321 catacgcgct gatgttgagg ctgccgcgtt tggtcttgat gatgtcgaag cccttcgccg
     4381 gggagaactc gccggcgatg gtgttgggat gaatgtcctc cggcgccgcg gctgtggtgt
     4441 cggggatggc cgcctcgtcc tgcgcg
//
LOCUS       DASPGK010000158         8715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_627201, whole genome shotgun sequence.
ACCESSION   DASPGK010000158 DASPGK010000000
VERSION     DASPGK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627201"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VEC56_04560"
     CDS             <1..211
                     /locus_tag="VEC56_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13456.1"
                     /translation="DLNKFLEKGEVASNPILRPGDTVAVGRKGFTTQELTTILAVITT
                     LGTVALLYFTIQNEIEDNEAQNNTN"
     gene            229..1896
                     /locus_tag="VEC56_04565"
     CDS             229..1896
                     /locus_tag="VEC56_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HXV13457.1"
                     /translation="MRKITESNEPEAGFRLNIGDYARVLWRKKYFLVVPLLISVAVTA
                     VGSRFLVPEYESSAIVRISSTRDMGEMERFIGMSRSRDDEVGAQLEADLLGSAFLDEL
                     IRSVGMDRDPDLIELAEYQRQNLYPGVTTEDLVMRRLRNFLQRHISLHREGPSMWRIS
                     YADANPEAAYVIADAMTRLYIERKRRLAMRDIQEVSQFSEEQLAVYKERLDRSVRDLE
                     RFQEQMASRGTEANPVTQSNISLAEKIRTDLDFTIRNAETTQQRIRERLVAVLGGVPS
                     GERIFSDPEIRKLESNLAARREAELLADLSVTPTPTPLSESDDIVESQQAIQHHLGTL
                     VAEKLSDVPYDYRPLVVEYFYQQIQVNAYRQKLSRLETYNRAFRAQVQLAPQQDTELS
                     RLRQEVEHNREVYNTFRATQTSTQISEAAQSTDIGATVALVEAASRPLEPVRPNKIKI
                     LALAFVLGAGIGASGLLLTEFSDTSFRSVDDVEKLLGLKVLGTVPRFEKTRWFHDSAR
                     KRAIIWTITCVVATSAALTAFYLFGKSTREQLIELDIDNAAAAPREP"
     gene            1900..2604
                     /locus_tag="VEC56_04570"
     CDS             1900..2604
                     /locus_tag="VEC56_04570"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HXV13458.1"
                     /translation="MATATDKKIPTIFDLYDGESPVATELRRLYHNAKRREDGGQNRT
                     FLVTSSNRGEGKSTISSWFAMTVAQFPTKKVVIVDTDLRRPRIHSIFGLNNTIGFKDC
                     LADHVDPMNVLKKTPLPNLHVITAGDRTPQPGKLFESDHLRDVLNKLAFYFDIVIVDS
                     PPVLAVSDTLFLCSEIESVLLVVLAGVTPRQVVTRARNVLLDSSARIGGVILNNASQA
                     LPYYYDYKYYGAQREK"
     gene            2608..3867
                     /locus_tag="VEC56_04575"
     CDS             2608..3867
                     /locus_tag="VEC56_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13459.1"
                     /translation="MTPEVGHRVDASISAPGANPRRRLRVLVSSGIFPNRVLVNRGIY
                     NLKAATALTRYCDVRVVAPVPYLPSFLRAPGYEWYSQVPRRDDVGSFHVEYPRYFVTP
                     KVGRSLHGIFLYLSVRSLYRDILRQFAPDVILGYFAYPYGFANVLLGRAAGVPVVTFC
                     RGSDIHSIARKRSQARLIAWALRRSAKVFAVSESLKSDIVALGVAPSHVVVIPNGIEP
                     ERFTRVGREAARARVGLPASGPVVVCVSRLSHEKGVDVLVDAAALLKTPGARVVIVGD
                     GPEEISLKARAQRAGVGDRVLFAGARPHADVPLWIAAADVAALSSRKEGHPNALVEYL
                     ACGRPAVATRVGGVPEILTSRDFGTMVEPDDAPALAVALDEALARSWNEEAIAQAGCA
                     RDWDAVARDIVRELEPVVAARATVGSA"
     gene            3864..4976
                     /locus_tag="VEC56_04580"
     CDS             3864..4976
                     /locus_tag="VEC56_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV13460.1"
                     /translation="MTTRGVKLKVAIVGCGKIADAHVEEVGKLAAAEVVAVCDREPLM
                     AEQLAARYGVPRHFSDFAALLTEVRPDVVHITTPPQSHLALAAQSLDAGCHVYIEKPF
                     ALNRAESLTIIEHARRVARKVTVGHSFAFDPLTVELRRLRDEGFLGDTVHVESHLGYN
                     LSGAFGAALMGDRAHWVHGLPGKLFHNNLDHVLHKIEDFIPEDAPTLVARATRRLPAG
                     FGDERDDLMDELRVLIEGAHATACATFSSHAVPVRHYARVWGTRNTVTADYVSRVLVV
                     EQGENYPSAIGRLLPAFAQARQYGRGGAKNIRRFMKSEFQFFTGMNELFRRFYHSIQD
                     DGEPPIAYDRIVRISGWMDDVFAQVNAALPRREVGR"
     gene            4973..5983
                     /locus_tag="VEC56_04585"
     CDS             4973..5983
                     /locus_tag="VEC56_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV13461.1"
                     /translation="MKRLVTGAGGFLGQAVVERLLALPNADLRCVVRSAAQAERLREK
                     AAAHPGARLEVVTGNLLSRADVARAVDGIDVLYHLAASLKGSPADIFMNTVVASNNVL
                     DALAGRPVTIVVVSSMGVYGTANLARGAVVDETTPLEPHPERRDVYSHAKWRQEKLFR
                     DRCAHAGHRLIVIRPGVIYGPGGPPMSARVGLNLFGLFLHLGGGNRIPLTHVENCADA
                     IVTVSENPDAVGHAFNVVDDDLPTSRAYLRRYRREVTRVRSVRIPYAGMLCVSRGVEW
                     YHRHSRGQLPAVFTRYKTATLWKGTRFDNTKLKCIGWTPRVGTREGLTKSFAYLRAAS
                     KG"
     gene            5987..7135
                     /locus_tag="VEC56_04590"
     CDS             5987..7135
                     /locus_tag="VEC56_04590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13462.1"
                     /translation="MVEPRDSDSPVPLVHVVQNLAIGGLERVVLNLATHAHRERYRTA
                     IVCLGAGGPLVEQARDAGVGVSVLAQGSGFRPGTVLGLARAFRAQRVGVVHCHNAAPL
                     VYGALAARMSGAAVVYTAHGMKTSGARQPVRLARLGLVDEFVTVSEDARRIATETAGV
                     DPARVRTIWNGVDTAAYRRIEGEARARVRRSLGLPEDAFVFAIVARLSAAKDHANLFR
                     AFAALTTSDANARLVVVGDGELRGSLGELVRELKMEQRIHMVGARDDVAAIAGAFDCF
                     VLSSYTEGLAMTLLEAMAAELPVVATRVGGNAEVVVDGETGRLVPARDSDRLRDAMQW
                     MSDHREAARAMGAKGRARAQSRFSVEAMVAAYEDAYRRALARRGHGHR"
     gene            7144..8310
                     /locus_tag="VEC56_04595"
     CDS             7144..8310
                     /locus_tag="VEC56_04595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13463.1"
                     /translation="MLRNQDIVYIGNDWFADNRTSSHQIARLLARHNRLLYVEAAGQR
                     APTASKRDLAKIVSKLKKAWADPVRVADNVHVYSPLIVPFHGSALARRVNRVALGASI
                     RRACHKVGFERPILWIILPHYSTIVDTVPHKGVVYYVVDEYSAMPGVDPDRIRAMEAL
                     VLGKADVVFAISEQLVTSKGRANPHTYFSPHGVETAHFARAYNEQLDRPRELEGIARP
                     VVGFFGLIEDWIDFDLLEYLARARPNYSFVMVGRVARDIARFAKVPNVFFLGKKSYEE
                     IPSFLRAFDVCHIPFKLNDVILHSSPLKLKEYLAGGKPVVSVAIDEVKKLDGLAYVAD
                     DYAGYLAAIDRALAEDSPQRAAERVRAMERESWESKVESISARVAAHVPGVRND"
BASE COUNT         1453 a         2937 c         2880 g         1445 t
ORIGIN      
        1 cgacctcaac aagttcctgg agaagggcga agtcgcgagc aatccgatcc tgcgcccggg
       61 ggacaccgtg gccgtggggc gcaagggctt caccacccag gagctgacga ccatcctggc
      121 cgtgataacc accctgggaa cggtggccct gctctacttc acgatccaga acgagatcga
      181 agacaacgaa gcgcagaaca acaccaacta gggtgcctgt agtacgccgt gcgcaagatc
      241 accgaatcca acgaacccga agccggcttt cgcctcaaca tcggcgatta cgcccgcgtt
      301 ctgtggcgca aaaagtactt cctcgtcgtt ccgctgctca tctcggtggc ggtcaccgcg
      361 gtcggctcac gcttcctggt accggagtac gagtcgagcg cgatcgtgcg tatttcgagc
      421 acccgcgaca tgggcgagat ggagcgcttc atcggcatgt cgcgctcgcg cgacgacgag
      481 gtgggggcac agctcgaggc cgacctgttg ggcagcgcct ttctcgacga gctgatccgc
      541 tccgtcggca tggaccgcga ccccgatctc atcgagctgg cggaatatca gcgccaaaac
      601 ctctacccgg gagtgacaac cgaggatctc gtcatgcgac gcctgcgcaa cttcttgcag
      661 cgtcacatca gcctccatcg cgagggcccc agcatgtggc ggatctcgta cgccgacgcc
      721 aacccggagg ccgcgtacgt catcgccgat gcgatgactc ggctctacat cgagcgcaag
      781 cgtcggctcg cgatgcggga catccaagag gtgagccagt tcagcgagga acagctcgcc
      841 gtctacaagg agcgtctcga ccgatccgtg cgtgatctcg agcgcttcca ggagcaaatg
      901 gcgtcgcgcg gcactgaggc caacccagtt acgcagtcca acatctcgct ggcggagaag
      961 attcgaaccg atctcgactt caccattcgc aacgcggaga ccacacagca gcgcatccgc
     1021 gagcggctgg tcgcggtgct aggaggcgtc cccagcggcg agcgcatctt ctcggatccg
     1081 gagatccgca agctcgagag caacctggcc gcgcgccgcg aagccgagct cttggccgac
     1141 ctctcggtga cccccacgcc cacgccgctc tccgaatccg acgacatcgt cgagagccag
     1201 caggcgatcc agcaccacct cggaaccctg gtggccgaga aactttccga cgtcccctac
     1261 gactaccgtc cgctggtggt cgagtatttc taccagcaga tccaagtgaa cgcgtaccgc
     1321 cagaaactct cgcgcctcga gacctacaac cgggcctttc gcgcgcaggt gcaactcgcg
     1381 ccgcagcagg ataccgagct ctcgcgcctg cgccaggaag tcgagcacaa ccgcgaggtc
     1441 tacaacacgt ttcgcgccac ccagacctcg acccagatca gcgaggcggc ccagagcacc
     1501 gacatcggcg ccaccgtcgc gctggtcgag gccgccagcc ggccgctcga gccggtgagg
     1561 cccaacaaga tcaagatcct cgccctggcg tttgttctgg gcgccggcat cggcgcgtcc
     1621 ggactcctgc tcaccgaatt ctccgatacg tcgttccgct cggtcgacga cgtcgagaag
     1681 ctactcggtc tcaaggtcct gggcacggtg ccgcgcttcg agaagacgcg ctggttccac
     1741 gactcggcgc gtaagcgcgc gatcatctgg accattacct gcgtggtcgc gacctccgcc
     1801 gcgctcaccg cgttctatct gttcggaaaa tcgacccgcg agcagttgat cgagctcgat
     1861 atcgacaacg cggccgcggc accccgggag ccttgaacca tggccaccgc caccgacaag
     1921 aaaatcccga ccatcttcga tctctacgac ggcgagtcgc cggtcgcaac cgagttgcgc
     1981 cgcctctatc acaacgccaa gcgccgcgag gacgggggac agaaccgcac cttcctggtc
     2041 accagttcca accgcggaga gggaaagagc actatctcgt cgtggttcgc gatgaccgtc
     2101 gcgcagtttc cgaccaagaa ggtcgtcatc gtcgacaccg acctgcgccg tccgcgcatc
     2161 cattccatct tcggtctcaa caacacgatc ggcttcaagg actgcctggc cgaccacgtc
     2221 gaccccatga acgtcctgaa gaagacgccg ctgcccaacc tgcacgtgat cacggccggc
     2281 gaccgcacgc cgcagccggg caagctgttc gagtccgacc acctgcgcga cgtgctcaac
     2341 aagctcgcct tttacttcga catcgtcatc gtcgacagcc ctcccgtact ggcggtcagc
     2401 gacacgctgt tcttgtgctc cgagatcgag tcggtgctgc tcgtggtact ggccggcgtc
     2461 acgccgcgcc aagtggtgac gcgcgcacgc aacgtgctat tggattccag tgcgcgcatc
     2521 ggcggtgtga tcctcaacaa cgcctcccag gcgcttcctt actactacga ctacaagtac
     2581 tacggcgcgc aacgggagaa gtagccggtg acgccggaag ttggtcatcg cgtggacgca
     2641 tcgatatcgg cacccggcgc gaacccgcgc aggcgcctgc gcgtgctggt atcgtccggg
     2701 atatttccca accgggtgct cgtgaaccgc ggtatttata acctcaaggc cgccaccgca
     2761 ctcacgcgct actgcgacgt gcgcgtggtg gcgccggttc cgtacctgcc gtcattcctg
     2821 cgcgcgcccg gctacgaatg gtattcccaa gtgccgcgcc gcgacgacgt ggggagcttc
     2881 cacgtcgagt acccgcgcta tttcgtcacc ccgaaggtcg gccgcagctt gcacggcatc
     2941 tttctctacc tgtcggtgcg ctcgctctac cgggacatcc tgcgccaatt cgcccccgac
     3001 gtgatcctcg ggtatttcgc gtacccctac ggattcgcga acgtgctgct cgggcgcgcg
     3061 gccggtgttc cggtcgtcac cttctgccgc ggaagcgaca tccacagcat cgcgcgcaag
     3121 cgatcgcagg cgcgtctgat cgcgtgggcg ctgcggcgct cggccaaggt gtttgcagtg
     3181 agcgagtcgc tcaagtcgga catcgtggct ctcggcgtcg ccccgtcgca cgtggtcgtg
     3241 attcccaacg gtatcgagcc cgagcgcttc acgcgcgtgg gtcgtgaggc cgcgcgcgcg
     3301 cgggtcggtt tgcccgcgtc ggggccggtg gtggtgtgcg tgagccgctt gagtcacgag
     3361 aagggcgtcg acgtgctggt cgacgcggcg gcactgctca agaccccggg cgcgcgcgtg
     3421 gtcatcgtgg gcgacggacc cgaggagatt tcgttgaagg cgcgcgcgca acgggccggg
     3481 gtgggcgatc gcgtcctgtt cgcgggcgcg cgaccgcacg cggacgtccc cctgtggatc
     3541 gcggccgccg acgtggcggc gctctcgagc cgcaaggagg gccaccccaa cgcgttggtc
     3601 gagtacctcg cgtgcgggcg tcccgcggtc gccacgcgcg tggggggcgt gcccgagatc
     3661 ctgacctcgc gcgacttcgg aaccatggtc gagcccgatg acgcgcccgc gctcgcggtg
     3721 gcgctcgacg aggcgctcgc gcgctcgtgg aacgaggagg cgatcgcgca agccggttgc
     3781 gcgcgggact gggacgcggt ggcacgcgac atcgtgcgcg agctcgagcc cgtggtggcg
     3841 gcgcgcgcga cagtagggtc ggcatgacca cccggggcgt caaactcaag gtggcgatcg
     3901 tgggctgcgg caaaatcgcc gacgcgcacg tcgaggaagt ggggaagctc gccgccgccg
     3961 aggtggtcgc ggtgtgcgac cgcgagccct tgatggccga gcagctggcg gcgcgctacg
     4021 gagtccctcg tcacttctcc gatttcgcgg ccttgctgac cgaagttcgt cccgacgtgg
     4081 tccacatcac gacgccgccg cagtcgcacc tcgcactggc cgcgcagtcg ctcgacgccg
     4141 gctgtcacgt ctacatcgag aaaccgttcg cgctcaatcg tgcggaatcg ctgaccataa
     4201 tcgagcacgc gaggcgggtc gcgcgcaagg tgacggtcgg gcactccttc gccttcgacc
     4261 cgttgaccgt cgagttgcgt cgcctgcgcg acgaaggctt cctcggcgac accgtgcacg
     4321 tcgagagtca cctcggttac aacctctcgg gtgccttcgg agcggccctg atgggtgacc
     4381 gcgcgcactg ggtgcacggg ctgcccggaa agctcttcca caacaacctc gaccacgtgc
     4441 tccacaagat cgaggacttc atcccggaag atgcgcccac gctcgtcgcg cgcgccacgc
     4501 gccggttgcc ggccgggttc ggcgacgagc gcgacgacct catggacgag ctgcgcgtct
     4561 tgatagaggg cgcgcacgcc accgcgtgcg ccaccttctc gtcgcacgcg gttccggttc
     4621 gccactacgc gcgcgtgtgg ggaacccgca acaccgtcac cgccgactac gtgtcgcgcg
     4681 tgctggtggt ggagcagggc gaaaactacc cgagtgcgat cggacggttg ctgccggcgt
     4741 tcgcgcaggc gcgccagtac gggcgcggcg gggccaagaa catccggcgc ttcatgaagt
     4801 ccgagttcca gttcttcacg ggtatgaacg agctatttcg tcggttctat cacagcatcc
     4861 aggacgacgg cgagccgccc atcgcgtacg atcgcatcgt gcgcatctcg ggatggatgg
     4921 acgacgtctt cgcgcaggtg aacgccgccc taccacgacg ggaggtgggg cgatgaagcg
     4981 tttggtcacc ggggcgggtg gattcctcgg gcaagccgtg gtggaacggc tgctggcgct
     5041 cccgaacgca gatctccgct gcgtggtgcg ctcggcggcg caggcggagc gcttgcgcga
     5101 gaaagccgcc gctcaccccg gcgcgcgact cgaggtcgtg accggcaacc tgctctcgcg
     5161 cgccgacgtc gcgcgtgcgg tcgacggcat cgatgtcctg tatcacctgg ctgcgtccct
     5221 caagggctcg cccgccgaca ttttcatgaa cacggtggtg gcgtcgaaca acgtgctcga
     5281 tgcgctcgcc ggccgtccgg tcaccatcgt ggtcgtgagt tcgatgggcg tgtacgggac
     5341 cgcgaacctc gcgcgcggcg ccgtcgtcga cgagaccaca ccgctcgagc cgcacccgga
     5401 gcggcgcgac gtgtattcgc acgccaagtg gcgccaagaa aagctgtttc gcgaccgttg
     5461 cgcgcacgcg gggcatcgtc tgatcgtgat tcggcccggc gtcatctacg gccccggcgg
     5521 gccgcccatg tcggcgcgcg tcgggctgaa tctgttcggt ctctttctcc atttgggagg
     5581 aggcaatcgc attcccctga cccatgtcga aaactgcgcg gacgcgatcg tgaccgtgtc
     5641 ggagaacccg gacgcggtgg ggcacgcgtt caacgtggtc gacgacgacc tgccgacctc
     5701 gcgcgcgtac ctgcgccggt accggcgcga ggtgacgcgc gtgcgctcgg tgcgcattcc
     5761 ctatgccggg atgctctgcg tctcgcgggg cgtggaatgg taccaccgcc actcgcgcgg
     5821 ccagctaccg gccgtgttca cgcgctataa gaccgccacg ttgtggaagg gtacgcgctt
     5881 cgacaacacc aagctcaaat gcatcgggtg gacgccccgg gtgggaacgc gcgaaggcct
     5941 cacgaagagc ttcgcgtacc tgcgcgccgc gtcgaaggga tgacgcgtgg tggagcctcg
     6001 cgattccgac tcgcccgtgc ccctcgtgca cgtggttcag aatctcgcga tcgggggact
     6061 cgagcgggtc gtcctcaatc tggcgaccca cgcccaccgc gagcgttatc gcaccgccat
     6121 cgtgtgtctg ggtgcgggcg gacccctggt cgagcaggcg cgcgacgcgg gcgtcggggt
     6181 gtcggtgctc gcccaaggct cgggttttcg gccgggcacg gtgctgggac tcgcgcgcgc
     6241 attccgcgcc cagcgcgtcg gcgtcgtcca ttgtcacaac gccgccccct tggtctacgg
     6301 cgccttggcg gcgcgcatgt cgggcgccgc ggtggtgtac accgcgcacg gtatgaagac
     6361 atcgggcgcg cgccaaccgg tgcggctggc gcgcctcggg ctggtcgacg aattcgtcac
     6421 cgtgtccgaa gacgcgcggc gtatcgcgac cgagaccgcc ggcgtggacc cggcccgcgt
     6481 gcgcacgatc tggaacggcg tcgacaccgc tgcctatcgc cgcatcgagg gtgaggcacg
     6541 cgcacgcgtg cgccgttcgc tcgggctgcc ggaagacgcg ttcgtgttcg cgatcgtggc
     6601 gcgcctctcg gcggccaagg accacgccaa cctgttccgc gccttcgccg cgctcacgac
     6661 gtccgacgcg aacgcgcgct tggtcgtggt gggggacggc gaactgcgcg ggtcgctggg
     6721 ggaactcgtg cgcgaactga agatggagca acgtatccat atggtcggcg cgcgcgatga
     6781 cgtggccgcg atcgcgggcg cgttcgattg cttcgtgctg tcgtcctaca cggaagggtt
     6841 ggcgatgacg ctgctcgagg ccatggcggc cgaactgccg gtggtcgcca cacgcgtggg
     6901 cggcaacgcc gaggtcgtgg tcgacggcga gacgggtcgc ttggtgccgg cgcgcgattc
     6961 cgaccggctg cgagacgcga tgcagtggat gtcggaccac cgcgaggcgg cgcgcgccat
     7021 gggggcaaag ggccgcgcgc gcgcgcaatc gcgcttcagc gtcgaggcaa tggtggcggc
     7081 gtacgaggac gcgtaccggc gggcgctcgc gcgtcgcgga cacgggcatc gctaggacgg
     7141 aagatgctgc gcaatcaaga catcgtctac atcggcaacg attggttcgc cgacaatcga
     7201 accagcagcc accagatcgc gcgcctgctg gcccgccaca accgcctgct ctacgtcgag
     7261 gcggcgggcc agcgcgctcc caccgcgtcc aagcgcgacc tggccaagat cgtgtccaag
     7321 ctcaagaagg catgggccga cccggttcga gtcgcggaca acgtgcacgt gtactcgccg
     7381 ctgatcgtgc cgttccacgg ctcggcgttg gcgcgccgcg tcaatcgcgt ggccctgggc
     7441 gcgtccatcc ggcgcgcgtg ccacaaggtc ggtttcgagc gccccatcct gtggatcatc
     7501 ctgccgcatt acagcaccat cgtcgacacg gtgccgcaca aaggcgtggt ttactacgtg
     7561 gtcgacgagt actccgccat gcccggcgtc gaccccgacc ggattcgcgc catggaagcg
     7621 ctcgtgctgg ggaaggccga cgtcgtgttc gccatctcgg aacagctggt gaccagcaag
     7681 ggacgcgcga acccgcacac ctacttcagc ccgcacggcg tcgagacggc gcacttcgcg
     7741 cgtgcctata atgagcagct ggatcggccg cgcgagctgg agggcatcgc acgaccggtg
     7801 gtcggcttct tcggcttgat cgaggactgg atcgacttcg atctcctcga gtacctggcg
     7861 cgcgcgcgac cgaactactc gttcgtgatg gtggggcgcg tggcccgcga catcgcgcgc
     7921 tttgcgaagg tgccgaacgt cttcttcctc ggtaagaaat cttacgaaga gattccgtcg
     7981 ttcttgcgcg ccttcgacgt atgccatata ccattcaagc tgaacgacgt catcctgcac
     8041 tccagccccc tgaagctcaa agagtacctg gccggcggca agccggtggt ctcggtggcg
     8101 atcgacgagg tcaagaagct cgacggtctc gcgtacgtcg ccgacgacta cgcgggctac
     8161 ctagcggcca tcgaccgcgc gctggccgag gattcgcccc agcgcgccgc ggagcgcgtg
     8221 cgcgccatgg agagggaatc gtgggagtcg aaagtggagt ccatctcggc gcgcgtggct
     8281 gcccacgtac cgggtgtccg caacgactga ggcaaggtgc aggctgcggc cggtcccacc
     8341 gcaatgcgca cgaaggacgc gggggtggtc ggatctcgag cgcgggtcgc aacgacgacg
     8401 ccgttgcgtc ggcgcggggc gaaactcttt gccgccgatg ccgaagtcgt tctacgctta
     8461 cgcggtggcg cgttttggac gcccatcgtg gaggcgggtg tgatcgccct tacctcgccg
     8521 gccgacggtc ggtcgacgcg gggtacgata tttgctgata tgagaccgat aaaccactgt
     8581 caatcaacgg gtaacatgac ccgacccgag gggcaatcgg acgcgcgaag gacctcttcg
     8641 gcgtcgtttg tcgttttcgg tcaatcaagg agtgggcgat gagaccaacc accacgtgca
     8701 ggaacctgac tctct
//
LOCUS       DASPGK010000159         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_744441, whole genome shotgun sequence.
ACCESSION   DASPGK010000159 DASPGK010000000
VERSION     DASPGK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744441"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEC56_04600"
     CDS             <1..261
                     /locus_tag="VEC56_04600"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXV13464.1"
                     /translation="EAVLLALGLCRELGARHVMLCLDSELVVRQVDGSYKVKHPELQR
                     LHARVHELMREFDSVEVEHVPREKNKSTDKLVNAALDGKELD"
     gene            295..663
                     /gene="rnpB"
                     /locus_tag="VEC56_04605"
     ncRNA           295..663
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEC56_04605"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            681..2438
                     /gene="recJ"
                     /locus_tag="VEC56_04610"
     CDS             681..2438
                     /gene="recJ"
                     /locus_tag="VEC56_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXV13465.1"
                     /translation="MNKYRWVVKEGGDDAAARELARSLDLPPAAARLFASRAFTDPTQ
                     VRAFLDPSRVDTHDPFLFERMREAVALIDATLRAKAPILVHGDYDVDGISGAALLFRY
                     LNRGDSPIHRFVPDRRKDGYGIAERAIEWALEQKVGLFIAVDCGTSDAARLRRLESAG
                     IPVIVCDHHLLPVDGDVAGVLLNPMRPGESYPFPLLCGTGVAFKLVQALEASGIRGAE
                     STESLVDLVALATVADVAPLIGENRYFTRVGLDRMNRSPRAGVEALRNFSRLSAGAIT
                     TRHLSFAFAPRLNAPGRVSRPKPALEILCVDAKDRAFQLAGILEAENERRRELTQRVQ
                     DEATAVITDMHDRDTRGGFVLANDGWDEGVLGIAAARLAEQFGRPTILMGSSGGLLKG
                     SGRSIPGVDLKAQLDQFREHFVRYGGHAQAVGLTMEPQHLPRFSAGFAERLHDVVQPE
                     RGLPLFIDASLSIDEVNLDLLGFLARCEPFGAGNEEPVWLIRDVQVARETSLVGDGHL
                     KLFFHDASGARASAIAFGWDRPQSPDDLHGRAIDLAVRVRKNTYQGSVYPDLRLVDLM
                     EAGTEHARAASAAARGQAE"
     gene            2440..>2565
                     /locus_tag="VEC56_04615"
     CDS             2440..>2565
                     /locus_tag="VEC56_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006720907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXV13466.1"
                     /translation="MRAVVQRVAEASVRVDGREVAGIGRGFLVLAGFSRADTRTTI"
BASE COUNT          417 a          846 c          896 g          406 t
ORIGIN      
        1 gaggccgtcc tgctcgccct gggactgtgc cgcgagctcg gcgcgcgcca cgtgatgctg
       61 tgcctggaca gcgagctcgt cgtgcgccag gtcgacggga gctataaggt caaacacccc
      121 gagctgcaac gcctccacgc acgcgtgcac gaactcatga gggaattcga ctcggtcgag
      181 gtcgagcacg taccgcgcga gaagaacaaa tccaccgaca agctcgtcaa cgccgctctg
      241 gatgggaagg aactcgacta acccgttcgg gattcatata atgcggttgg gaaccgagcg
      301 ggcgagacgg tcgcgggcgg tcttgaaccg taccgaggaa agtccgagca ccgcggagca
      361 ggatgctggg taacgcccag cggaagcgat tccagggaca gtgccacaga aaacataccg
      421 cccaagctcg gggcaacccg ggacggcaag ggtgaaaagg tgaggtaaga gctcaccggg
      481 ctcgcggcga cgcgggccgt ccgggtaaac cccatccggt gcaagaccaa ataggagagc
      541 agaggcgctc cgcctcgcca cgactctcgg gttggttgct cgaggtgtcc ggtaacgggc
      601 atcccagata aatgatcgtc ctccttcggg ggaacagaac tcggcttacg tcccgctcgg
      661 tcccgttcta caatcgacgc atgaacaagt accgctgggt ggtgaaggag gggggcgacg
      721 acgcggctgc gcgcgagctc gcgcgctcac tcgacctccc gcccgcggcg gcgcgcctgt
      781 tcgcgtcgcg cgcgttcacc gacccgaccc aagtgcgcgc gttcctcgat ccctcgcgcg
      841 tcgacaccca cgaccccttc ctcttcgagc gcatgcgcga agcggtcgcg ttgatcgacg
      901 cgacgctgcg cgcgaaagcg cccatcctcg tccacggcga ctacgacgtc gacggcatct
      961 cgggcgccgc gctcctgttt cgctacttga accgcggcga ctcgccgatt caccgcttcg
     1021 tcccggaccg gcgcaaggac ggctatggga tcgccgagcg cgccatcgaa tgggcgctcg
     1081 agcagaaggt cggactcttc atcgcggtcg actgcgggac gtccgacgcg gcgcggctgc
     1141 gccgcctgga gagcgccggc atccccgtca tcgtgtgcga tcaccatctc ttgcccgtcg
     1201 acggcgacgt cgccggcgtg ctcctgaacc cgatgcgccc cggcgagagc tatccgtttc
     1261 cgttgctgtg cgggaccggg gtcgcgttca agctggtgca ggcgctggaa gcgagcggga
     1321 ttcgcggcgc cgagagcacg gagtcgctgg tcgacttggt ggcgctcgcg accgtcgccg
     1381 acgtggcacc cttgatcggc gagaaccgtt acttcacgcg cgtgggattg gatcgcatga
     1441 accgctcccc gcgcgcgggg gtcgaagcgc ttcgcaactt ctcgcggctg tccgcgggtg
     1501 ccatcaccac gcggcacctg tcgttcgcgt tcgcaccgcg gttgaatgcg cccgggcgcg
     1561 tgtcgcgacc caagccggcg ctcgagattc tgtgcgtcga cgccaaggat cgcgcgtttc
     1621 agttggcggg gatcttggaa gcggagaacg aacgccgccg cgaattgacg cagcgcgtcc
     1681 aggacgaggc caccgcggtg atcacggaca tgcacgaccg cgacacgcgc gggggcttcg
     1741 tgctcgcgaa cgacgggtgg gacgaaggcg ttctgggcat cgcggccgcg cgcctggccg
     1801 agcagttcgg gcgcccgacc atcttgatgg gctcgagcgg cgggttgctc aaggggtcgg
     1861 gccggagcat cccgggcgtg gacttgaaag cgcagctcga tcagtttcgc gagcacttcg
     1921 ttcgctacgg cggacacgcg caggcggtcg ggctcacgat ggagccccag cacctgccgc
     1981 gtttctcggc cggcttcgcc gagcgcctgc acgacgtcgt gcagccggag cgaggattgc
     2041 cgctcttcat cgacgcgtcg ctttccatcg acgaggtcaa tctggatctg ctgggtttcc
     2101 tcgcgcgctg cgagcccttc ggcgccggca acgaagagcc ggtatggctc atccgcgacg
     2161 tccaagtcgc gcgcgagacg tcactggtcg gcgacgggca cctgaagctc ttcttccacg
     2221 acgcgagcgg cgcgcgcgcg tcggccatcg cgttcgggtg ggaccgcccg caatcgcccg
     2281 acgacttgca cggtcgcgcg atcgacctcg cggtgcgcgt gcgcaagaac acctaccagg
     2341 gttcggtgta tccggacctg cgtctggtcg atctcatgga ggcggggacg gaacacgcgc
     2401 gcgcggcgag tgcggccgca cgcgggcagg cggagtgacg tgcgcgcggt cgtgcagcgt
     2461 gtggccgaag cgagtgtgcg cgtcgatggt cgcgaggtgg ccgggatcgg ccgcggattc
     2521 ctggtgctgg cgggcttttc gcgcgcggac acgcgcacca cgatc
//
LOCUS       DASPGK010000160         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_978267, whole genome shotgun sequence.
ACCESSION   DASPGK010000160 DASPGK010000000
VERSION     DASPGK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978267"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VEC56_04620"
     CDS             <1..1213
                     /locus_tag="VEC56_04620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13467.1"
                     /translation="TPPVNRAGDGSAPLSVLQSVQTWLPQTATWLYTQVRFLPPGIES
                     TIACERTAHLDQFPFPRIRCLKDAPTVSQIRDRALRRLGVRHHLSFTLDAGRRAGARV
                     VHSHFGVVGWRDLAVARRLGAAHAVTFYGVDVRHYPQSDPRWFGRYQEMFAQVDAVLC
                     EGPFMGQAIVDLGCPKEKLRIHHLGVDLAAIPFAPLAWEPGQPLRVLLSGSFREKKGF
                     PDAIDALGRVGNEVAVEITIIGDAHGLPSTVLEKKKILEALARNGLAERTRLLGYQPH
                     RVVMDEASRHHVFLSPSVTASDGDTEGGAPVSLIEMSAAGLMVVSTTHCDIPGVVIHG
                     KTGLLAPEHDIDALAAHLRWLVANPSLWRAMGAAARAHIEAEFDARRQGERLAAIYRE
                     IGAGHATGMVT"
     gene            1213..1872
                     /locus_tag="VEC56_04625"
     CDS             1213..1872
                     /locus_tag="VEC56_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV13468.1"
                     /translation="MARAAHSTWYNWYANGLLARNIEQLARGLSGSLVIDVGCGESPY
                     RAMLSGFARYVGFDAPGRPDSASHPDVVGDAGALPFRTASADAVLCTEVIEHVPDPAI
                     LLAEAHRVLVPGGSLLLSSPFTWHLHDEPHDYWRFTEYGLRLLLERAGFRVAAVRATN
                     GSFGALLQARAYWLYFVSGGMRPLTRPIVWLLQAAAVAAAPLDRNRRMASNYVVLARK
                     A"
     gene            1872..2906
                     /locus_tag="VEC56_04630"
     CDS             1872..2906
                     /locus_tag="VEC56_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXV13469.1"
                     /translation="MGRPLVFLLGTQRSGTTWLANIFDSHPACLHFYEPFAPAYRLFP
                     YFPGEFQWLAPPAPALAGRLRADLPRLVSYRSRLFDPVHATRRQFELEARLMAKLETA
                     YRRLHLTPLRFAAQFNLLHLNRLGQKPVAYFEKGEIEVAAIKDVRLYFKATFLADALP
                     DARFIHVVRHPAAVVSSMDHQLKQGRLIEIAAHIDRFVPEIRAQEPLARYQPVLVRVR
                     PGHRLDALCAYWRVANEELERQLTALPARALPLVYEDLATDPLARVTAMFAWSGLAMS
                     ASTDAYVRASSTSRTDRRTVLDTNRVSATYYRDWVSKVSPELLASVERVCGDSPLMAR
                     FAPFYDDAGR"
     gene            2916..4376
                     /locus_tag="VEC56_04635"
     CDS             2916..4376
                     /locus_tag="VEC56_04635"
                     /inference="COORDINATES: protein motif:HMM:NF007773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="HXV13470.1"
                     /translation="MSQDVDIKHLRRRATSGARWTAAATGVRVGLQFLQLAILARLLR
                     VEDFGVMAIANVAMAFAVTFSDAGVSNAIIHYRDAKREELSSLYWLNVLGGLVVFVAM
                     WFAAPLVARFYHQPTLEPVLRVAASVFIVGPLGQQFQVLFERDLHFGRLSVVEIASLV
                     AGAVVGIVLAVRGFGVWSLVWSSVLSSVLRSLGLAAIGWARWRPMLHFVPRECGRFLH
                     FGAYQMGEKTVNLAAQHLDKLVIGYLLGTGPLGYYELAYRFISRPHQIINSLFTRVAF
                     PVFSAVQHDRDRLRKGYLELIEAIAAIMIPLHLAMFALAEPLVGVQLGPRYETTVALL
                     QILWIAGMAFAITSPVGTLLLACGRADIGFWINVVRTTLVIVSVFVGARWGVVGIAWA
                     LVVVVVAVMFPAQAYVRWLVVGMGWGEFVSRLTPFLAAASVAAAVCILARRVIAWPSD
                     LVELVVMLPVAGAVYLGLLWWRARPRVQRILRLVRL"
     gene            4420..>4688
                     /locus_tag="VEC56_04640"
     CDS             4420..>4688
                     /locus_tag="VEC56_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV13471.1"
                     /translation="MSNPVRSPVRTKDRFLVFGAPQIEQAEIDEVVHSIKSGWLGTGP
                     KVARFEEDFKAYKGAPHAAALYSCTAALHLAMVASGVGEGDQVITT"
BASE COUNT          620 a         1612 c         1580 g          876 t
ORIGIN      
        1 aaccccgccg gtgaaccgcg ccggcgacgg atccgcgccg ctttccgtcc tccagtccgt
       61 ccagacctgg ctcccccaga cggcgacgtg gctctacacg caggtccgct ttctgccgcc
      121 cggtatagag tcgaccatcg cgtgcgagcg caccgcgcac ctcgaccagt ttccattccc
      181 tcgcattcgc tgcttgaagg acgcgcccac cgtgtcccag attcgggacc gtgctctgcg
      241 caggctggga gttcgccacc atctctcgtt caccctcgac gccggtcgac gggcgggcgc
      301 gcgcgtggtg cactcgcact tcggagtcgt ggggtggcgc gacctcgcgg tggcgcggcg
      361 cttgggcgcg gcccacgccg ttaccttcta cggcgtcgac gtgcgccact atccgcagtc
      421 ggatccgcgc tggttcggtc gctaccagga aatgttcgcg caggtcgacg cggtcttgtg
      481 cgaggggccg ttcatggggc aggcgattgt ggatctaggt tgcccaaagg agaagctccg
      541 cattcaccat ctgggcgtgg acctcgcggc gattcccttc gcgccgcttg cgtgggagcc
      601 ggggcagccg ctgcgcgtcc tgctctcggg gtcgtttcgc gagaagaaag gcttcccgga
      661 cgcgatcgat gcgctgggtc gggtcgggaa cgaggtcgcg gtcgagatca cgatcatcgg
      721 cgacgcgcac ggcctgccta gcacggtcct cgagaagaag aagatcctcg aggccctcgc
      781 gcgcaacggc ctggccgagc gcacgcgcct gctcggctac cagccccatc gcgtcgtcat
      841 ggacgaagcg agtcgccacc acgtgttctt gagcccgagt gtcaccgcct ccgacggcga
      901 caccgaaggc ggcgcgccgg tgtcgctgat cgagatgagc gcggcgggtc tcatggtcgt
      961 gagcacgacg cactgcgata ttcccggtgt ggtaatccac ggcaagaccg gcttgctcgc
     1021 ccccgagcac gatatcgacg cgctcgcggc gcacctgcgc tggctggtcg cgaatccgtc
     1081 gctgtggagg gccatgggcg cggcggcccg cgcccacatc gaagccgagt tcgacgcgcg
     1141 ccgccagggt gagcgcctgg ccgcgatcta tcgtgagatc ggcgcggggc acgcgacggg
     1201 gatggtgacg tgatggcgcg cgccgcccat tcgacctggt acaattggta cgcgaacggc
     1261 ttgctcgcgc gtaacatcga gcagctcgcg cgggggctgt cgggatcgct cgtgatcgac
     1321 gtgggctgcg gcgagtcgcc ctaccgcgcc atgctctcgg gtttcgctcg ttacgtcggc
     1381 ttcgatgcgc ccggtcgccc cgacagcgcg agccacccgg acgtcgtggg cgacgccgga
     1441 gcacttccgt ttcgcaccgc gagtgcggac gcggtgttgt gcaccgaggt catcgagcac
     1501 gttcccgacc ccgcgatact gctggccgag gcgcaccgcg tgctcgtgcc cggcggctcg
     1561 ctcctgctct cgtcgccgtt tacgtggcac ctccacgacg agccgcacga ctactggcgt
     1621 ttcaccgagt acggcctgcg cctgctcctc gagcgcgcgg ggtttcgcgt cgccgccgta
     1681 cgcgccacca acgggagctt cggcgcgctc ttgcaggcgc gcgcgtactg gctctatttc
     1741 gtttcgggcg gcatgcgtcc gctcacgcga ccgatcgtgt ggcttttgca ggcggccgcg
     1801 gtggctgcgg caccgctcga tcgcaaccgg cgcatggcga gcaactacgt cgtcctggcg
     1861 agaaaggcgt aatgggtcgc ccgctggttt tcctgttggg aacgcagcgc tcgggcacga
     1921 cctggctcgc caacatcttc gattcgcacc ccgcgtgtct gcacttctac gagccctttg
     1981 caccggccta tcggttgttt ccgtattttc ccggcgagtt ccagtggctg gcgccccccg
     2041 cgcccgcgct ggccgggcgg cttcgggccg acctcccgcg cctggtctcg taccgctcga
     2101 ggctcttcga cccggtgcac gccacgcgac gccaattcga gctcgaggcg cggctcatgg
     2161 ccaagctgga aacggcgtac cggcgccttc atttgacgcc gctgcgtttc gccgcgcaat
     2221 tcaacttgct gcacttgaac cgcctcggtc agaaaccggt cgcgtatttc gaaaagggag
     2281 agatcgaagt cgccgccatc aaggatgtgc gcctgtactt caaggcgacc ttcctggccg
     2341 acgcgctccc ggacgcgcgc tttatccacg tggtacgcca cccggccgcg gtagtgagct
     2401 cgatggacca tcagttgaag caggggcgat tgatcgagat cgcggcccac atcgaccgct
     2461 tcgtgcccga gatacgcgcg caggagcccc tcgcgcgcta ccagccggtg ctcgtgcgcg
     2521 tgcggcctgg acaccgtctg gacgcgctct gcgcgtattg gcgcgtggcc aacgaagagc
     2581 tcgagcggca gctgacggcc ctgccggcgc gcgcgctccc cttggtctac gaagatctcg
     2641 ctaccgaccc cctcgcgcgc gtgacggcga tgttcgcctg gtccgggctc gcgatgtccg
     2701 catcgaccga cgcctacgtg cgcgcgtcgt ccacctcgcg caccgaccgc cggacggtgc
     2761 tggacaccaa ccgcgtctcg gccacctact atcgcgattg ggtgtcgaag gtctcgcccg
     2821 agttgctggc gtcggtcgag cgcgtgtgcg gtgacagccc gctcatggcc cgcttcgcgc
     2881 cgttctacga cgacgcgggg aggtgaaacg agtccgtgtc ccaggacgtc gacatcaagc
     2941 acctgcgccg acgcgcgacg tcgggggctc gttggaccgc cgccgccacc ggggtgcgcg
     3001 tcggcctgca gttcctccag ctcgcgatcc tcgcgcgctt gctgcgggtc gaagacttcg
     3061 gcgttatggc gatcgccaac gtcgcgatgg ccttcgcggt caccttcagc gacgccggtg
     3121 tcagcaacgc gatcattcac taccgcgacg ccaagcgcga ggaactgtcg agcctgtact
     3181 ggctcaacgt cctgggtggc ctcgtggtgt tcgtcgcgat gtggttcgcg gcgcccttgg
     3241 tggcgcgctt ctaccaccag cccacgctgg agccggtgct gcgcgtggcc gcctccgtgt
     3301 tcatcgtcgg tccgctgggc cagcaattcc aggttctgtt cgagcgcgat ctgcacttcg
     3361 ggcgtctgtc cgtggtcgag atcgcgtcgc tggtggcggg cgcggtggtg ggaattgtgc
     3421 tcgccgtgcg cggcttcggc gtgtggtcgc tggtgtggtc gtccgtcctc tccagcgtgc
     3481 tgcgttcgct gggcctcgcg gccatcggct gggcgcggtg gcgcccgatg ctgcacttcg
     3541 ttccccgcga gtgcggtcgc ttcctgcact tcggcgcgta ccagatgggg gagaagaccg
     3601 tgaacctggc ggcccagcac ctcgacaagc tcgtgatcgg gtacctgctc ggcaccggac
     3661 cgctcggcta ctacgagctg gcctatcgat tcatctcgcg gccgcaccag atcatcaact
     3721 cgctgttcac ccgcgtcgcg ttcccggttt tctccgcggt gcagcacgac cgcgaccgcc
     3781 tgcgcaaggg ctacctcgaa ttgatcgagg ccatcgccgc gatcatgatt ccgctccacc
     3841 ttgccatgtt cgcgctggcc gagcccctgg tcggcgtcca gctgggcccg cgctacgaaa
     3901 cgaccgtcgc gctgctgcag atcctttgga tcgcgggcat ggccttcgcg atcacgagcc
     3961 cggtcggcac gctcctcttg gcgtgcgggc gcgccgacat cgggttctgg atcaacgtcg
     4021 tgcgtaccac gctcgtcatc gtcagcgtgt tcgtgggggc gcgctggggt gtggtcggga
     4081 tcgcgtgggc gctggtcgtg gtggtggtcg cggtcatgtt cccggcccag gcgtacgtgc
     4141 gctggctcgt ggtcggaatg ggttggggag agttcgtttc gcgtctcacg ccgttcttgg
     4201 ccgccgcctc ggtggccgcg gcggtgtgca tcctcgcccg ccgtgtgatt gcatggccga
     4261 gcgacctcgt cgagctggtc gtcatgctgc cggtcgcggg cgcggtctac ctggggctcc
     4321 tgtggtggcg cgcgcgaccg cgagtgcagc gcatcctccg gctcgtgcgc ttgtgagcga
     4381 ttgcgcggct acacgccgcg tgatagtctt gctccttcta tgagtaatcc cgtccgttcg
     4441 ccagttcgca ccaaggaccg ctttctcgtc ttcggggcgc cgcagatcga acaagccgag
     4501 atcgacgaag tcgtccactc catcaagagc gggtggctcg gcaccggccc caaggtcgcg
     4561 cgcttcgaag aggacttcaa ggcctacaag ggcgcgccgc acgcagccgc cttgtattcg
     4621 tgcacggcgg cgctgcacct ggcgatggtg gcgtccgggg tgggcgaagg cgaccaggtc
     4681 atcaccac
//
LOCUS       DASPGK010000161         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_277701, whole genome shotgun sequence.
ACCESSION   DASPGK010000161 DASPGK010000000
VERSION     DASPGK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277701"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VEC56_04645"
     CDS             <1..405
                     /locus_tag="VEC56_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV13472.1"
                     /translation="GAGRMQELQKQYTEQGVVWLTICSSAEGKQGYYDSDALKGMVSE
                     KKLASTAYLRDVDGKVGKMYGAKTTPHMFVINPDGVLIYAGAIDDKPSPNPDDLAGAN
                     NYVTASLTTAMAGKPVDVKSTASYGCSVKYAN"
     gene            531..>2664
                     /locus_tag="VEC56_04650"
     CDS             531..>2664
                     /locus_tag="VEC56_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXV13473.1"
                     /translation="MRKLPLLTVALGVTLTALLIDGRADRNHLEVRVRTAASDVVVTP
                     SPLGAVVTAHPSAPTSLVPLEDAGKPAVPFRVVEVLIPRGERVSGVTATARATETIVR
                     DVVPALVAPLEPQPDAPRSTLAPAEAPVAPENGGDIYPSELVRYLGTGTWHGYSIASF
                     AVFPVRVGGGAVSLHTDVEVRIELTPGAENEATTRAVRATPRTAAEVTSRVRARVENP
                     EAMSTYSSPRTDRHAGAFAPSSLPSEDGSPVDYLIVTTAALAPAFQVLADWKTAKGVA
                     TVVRTVDWITANSRNGSDLPETIRFFLQDAYASWGIRYVLLAGDTPEIPPRYLYSAYH
                     YGGTSIPGDIYFACLDGTYNADGDTRFGEQPADAPDLYPELSVGRLPVSDPEDADVVV
                     GKIIGYETPLDAEFTDKVLMLSEVLFPSPWTSGEILLNGGDVTDVPYVLYVNSPERRT
                     VRMYETPWIYPGSVQESRALAIDSLEAGFNQVFHVGHGFRFNMHCADDNIAIPDADVL
                     QHPNRFFNLYMLNCTAAAFDYDCLGEHLLRNPEGGAVSIVGAVNSAFAQVAAVYLDNY
                     ARALYRDDVVRLGDTFAASRLPRTSLAVLGDNVDLWTHYIYTLLGDPEQAIWTARARV
                     PQVALPDSVPAGDSAIQVGVTVDGQPVPGALVCLRKEGEDYRVGETDAGGIVTMFFTA
                     PTAGAIDVVVTGPNLVRTAASIAVTGAIG"
BASE COUNT          422 a          955 c          844 g          443 t
ORIGIN      
        1 ggcgcgggga gaatgcagga attgcagaag cagtacaccg agcagggcgt ggtgtggctc
       61 accatctgct cgtcggctga aggcaagcag ggctactatg actccgacgc cctcaaggga
      121 atggtctcgg agaagaagct cgcgtcgacc gcttacctgc gcgacgtcga cggcaaggtc
      181 ggtaagatgt acggtgccaa gacgacgccg cacatgttcg tcatcaaccc cgatggcgtg
      241 ctgatttacg ccggcgccat cgacgacaag ccgtccccca acccggacga cctcgcgggg
      301 gcgaacaact acgtcactgc atccttgact acggccatgg cgggcaagcc ggtcgacgtc
      361 aagtcgacgg cctcctacgg ctgcagcgtg aagtacgcga actagcgtcc cctattgagg
      421 atgggccggc ggccgtgggt ggccgccagc cctcctcgcc ccccgatcgc cgccgttgac
      481 acaaccttgt aaatccctta cagttactcg cgcatctcgt gctggacgcc atgcgcaaac
      541 ttcccctgct cacggtcgcg ctgggggtta ccctcacggc gctcttgatc gacggtcgcg
      601 ccgaccggaa ccacctcgag gtccgggtcc gcaccgcggc gagcgacgtc gtggtcaccc
      661 cgtcacccct cggggcggtc gtcaccgcgc atccctccgc tccgacttcg ctcgttccct
      721 tggaggacgc gggtaagccc gccgtcccgt tccgcgtcgt cgaagtgctg atcccgcgcg
      781 gtgagcgggt gagcggtgtg accgcgacgg cgcgcgcgac cgagacgatt gtgcgcgacg
      841 tcgttcccgc gctggttgca cccctggagc cgcagcccga tgcgccgcga tccacccttg
      901 cgccggccga agcgccggtc gcgcccgaga acggcggcga catctatccc tcggagttgg
      961 ttcgctacct cggcaccggg acgtggcacg gctactcgat cgcgagcttc gccgtgtttc
     1021 ccgtgcgcgt cggcgggggg gccgtttcgc tccacacgga cgtcgaggtg cgcatcgagc
     1081 tcacccccgg tgctgagaac gaagctacga cgcgcgcggt gcgcgcaacc ccgcgcacgg
     1141 ccgccgaggt cacctcgcgc gtgcgggcac gggtcgagaa cccggaggcg atgtcgacgt
     1201 actcgtcgcc gcgaaccgac cgccacgcgg gtgcgttcgc tccctccagc ctcccgtccg
     1261 aagacgggag cccggtcgac tatctgatcg tcaccaccgc ggcgctggcc cctgcgttcc
     1321 aagtgctcgc cgattggaag acggccaagg gtgtcgccac cgtagttcgc accgtcgatt
     1381 ggatcacggc caactcgcgc aacggctccg acctgcccga gacgattcgc ttcttcctgc
     1441 aggatgccta cgcgagctgg ggcatccgct acgtgctctt ggccggcgac acgcccgaaa
     1501 tccctccgcg ctacctgtac agcgcgtacc actacggcgg cacctcgatc ccgggcgaca
     1561 tctacttcgc gtgcctggac gggacttaca acgccgacgg cgacacgcgc ttcggcgagc
     1621 agccggccga tgcccccgat ttgtatccgg agttgtccgt cgggcgcctg ccggtttcgg
     1681 accccgaaga cgccgacgtc gtagtcggca agatcatcgg ctatgagacg cccctcgacg
     1741 ccgagttcac cgacaaggtg ctcatgctgt ccgaggtgct gttcccgtcg ccgtggacgt
     1801 cgggagaaat cctgctcaac ggcggcgatg tgaccgacgt cccctacgtc ctgtatgtga
     1861 actcccccga gcggcgtacc gtgcgcatgt acgaaacccc ttggatctac cccggctccg
     1921 tgcaggagtc gcgcgcgctc gccatcgact cgctcgaggc cggattcaac caggtgttcc
     1981 acgtcggcca cggttttcgg ttcaacatgc actgtgccga cgacaacatc gccatccccg
     2041 acgccgacgt gcttcagcac ccgaatcgct ttttcaatct ctacatgctc aactgcaccg
     2101 cggccgcgtt cgactacgac tgcctgggcg agcacctgct gcgcaacccc gagggcggcg
     2161 cggtctcgat cgtcggtgcc gtgaacagcg cgttcgcgca agtagccgcg gtctacctcg
     2221 acaactacgc gcgcgcgctg taccgggacg acgtcgtgcg tctgggtgat acgttcgcgg
     2281 cctcgcggct tccgcgcacc tcgctcgcgg tgctcggcga caacgtcgat ttgtggacgc
     2341 actacattta cacgctgctc ggcgaccccg agcaggcgat atggaccgcg cgcgcgcgcg
     2401 tgccccaggt cgcgctgccc gacagcgtcc ccgccggcga tagcgccatc caagtcggcg
     2461 tgaccgtcga cggccagccg gtgccgggcg ccctcgtgtg cctgcgcaag gaaggcgaag
     2521 actaccgcgt cggcgagacg gacgcgggcg gtatcgtcac gatgttcttc accgcgccga
     2581 ccgccggcgc tatcgacgtg gtcgtgaccg gccccaacct ggtgcgcacc gcggcgtcga
     2641 tcgcggtcac cggcgcgatc ggac
//
LOCUS       DASPGK010000162         8694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1447061, whole genome shotgun sequence.
ACCESSION   DASPGK010000162 DASPGK010000000
VERSION     DASPGK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447061"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VEC56_04655"
     CDS             <1..1237
                     /locus_tag="VEC56_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13474.1"
                     /translation="MRRSMHSIHLVPSTAGRSIAIATLLALALIGIAVSARAQTANTI
                     QVRGLVDLVASDVGDYRYLNTNNTNDGNFDALRSRLFVDGKRGNTSVFLQFLISPESY
                     NEFRFYGGYVMQRIHEERNVFIEAGLIPVHDGIWASHTYSNKNPLMGIPLMQYWKSSL
                     PYTMMPNDIDDLLSQRGRGQEYGVSYADSNGTRGYRYASMPILYDNCWNYGAYTLGTL
                     GKLEFSLGITLGAPSAAVQGTDFNDNIATHAKFGFAFTPGFKVWGSYAHGAYLDRAVA
                     PYLPLGKTVNDYYQNLYGGSVDWKVGKFWFMSEYFYNHYDTPIRNDGLSNQSLYVQTV
                     FSMAAAWDLCLRWDALRFGEVQDSAGNTMTWDENVYRVESGVGYHVSRDLVVKAVAQM
                     TRFEGDWDLIPAIQASFGF"
     gene            complement(1370..3421)
                     /locus_tag="VEC56_04660"
     CDS             complement(1370..3421)
                     /locus_tag="VEC56_04660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV13475.1"
                     /translation="MSSPIAPKVRVGLVGAGYVATHHLRALNALPFVEVVGICDRDRT
                     RAEALAKKFRVRAVYTDLSSMTSAKPDVIHILTPPESHCSLSLAAMDMGCHVFVEKPM
                     AESVEECDRMIAHAREKGVVLSINHSDRFDPVVLKAIAVARSGSIGDVLAVHSIRSSD
                     YPTYAGGPLPQPYRQGSYPFRDLGVHSLYLIESFVGQVRTLDVNFYESGRNPMLTFDE
                     WRAYAECEHGTGYAYLSWNTRPIQSELEIHGTRGVIKVDRFLQVCDVNRVLPGPKQIG
                     NILNGARNAARRAFVIPLNLLRFMTGSLKPSPGIYRCVQDFHLALHNGTPVPVPAEEG
                     RRMAKWIDVASRDADHQKAVRLSHEMTKPLARSRVLVTGGGGFLGRALVERLRESGES
                     VRLLLRRAPAGAGDDVVAGSLGQPEVVERAVQGVEVVYHVGAAMKGGKEEFEQGTVWG
                     TRNVIDACLRHGAKLVYVSSMSVADHAGHTTGVPVTESSPYEPHPDRRGVYTQTKFRA
                     EQMVLEAIRDKGLRAVIVRPGQIFGPGAEHVTPNGVIGIAGRWIVAGKGDRNLPLVYR
                     DDVVDALLASESSPRALGHVVNVVDPTPITQNDYLAYAGRAQSLRVHRVPVWALMVLG
                     WKIEILGKLMKRDVPLTRYKIRSLEPLWPFDVTKARDVLGWSPRVGTMDGLKRTFGDR
                     V"
     gene            complement(3435..4412)
                     /gene="rfaD"
                     /locus_tag="VEC56_04665"
     CDS             complement(3435..4412)
                     /gene="rfaD"
                     /locus_tag="VEC56_04665"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HXV13476.1"
                     /translation="MAAPIIVTGGAGFIGSNLVDALNARGHDDVVVVDHVEGDPGKLR
                     NLERLQIGEYLDKREFRARVRAGKAPKASAVFHLGACSSTTETDEGYLRDNNTEYTRE
                     VCEWSLAGGARFVYASSAATYGDGSLGYSDEDAVTPTLKPLNLYGASKQWFDEWALRN
                     GVLKRVAGIKYFNVYGPYEDHKGDMRSLVNKAYAQVMREGEIALFKSYRPAFKDGEQR
                     RDFIHVDDAVAVTLFFLDRPEVSGLFNCGTGSARTWVDLAKALFSAVGLPPRIRFIDM
                     PESIREKYQYHTQADMTKLRAAGYSAPFLQVEDGVRRYVQEYLQPQSAH"
     gene            complement(4453..5238)
                     /locus_tag="VEC56_04670"
     CDS             complement(4453..5238)
                     /locus_tag="VEC56_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV13477.1"
                     /translation="MSTKTIAFVHGNFVSKRCWDPFVQRFEARGYKCVPIAYPLRDDT
                     VESLRGKHPDPALARLTLPEVIDHHVRTIQSLDEKPIIMGHSFGGLLTQLLMQRDVGA
                     AGVAIDSVPPQGVLGTQWSFVRSVFPAINPIKRHQPYLMPFEHFQYTFVNNMPPAAQR
                     EAYDRHVVPESRMLASGGLSSAAHVDFARKRAPLLLIAGSADHIMPAALNRTNYKRYK
                     GSAGVTDFKEFPGHSHYSIIAGPGFEEVADYALAWAERAVAKG"
     gene            5392..6633
                     /locus_tag="VEC56_04675"
     CDS             5392..6633
                     /locus_tag="VEC56_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV13478.1"
                     /translation="MNARLSRISQFLALRRSTVGMFAMVLLVGMGEEMAERFLPIYLL
                     AVGGSAMSIGVLQAMDNFLSALYSFPGGYLADRIGTRKALLVFNLMAMAGFAVVILIP
                     TWQAVLAGAVLFISWSAISAPATLSLIYKVLPPDKRTMGVSMVSIMRRIPKGLGPLMG
                     GLCIALWGEREGVRLAFIAAFVLALFAVVLQRRLIADDGPRARAAAGLALPLVEKDPR
                     RMFRLMSPEMRRLLFTDILVRFCEQIPYAFVVVWCMKSIPHPVTAVQFGVLSTIETVV
                     ALLCYIPVAHLADRGHKKRYVLITFGFFALFPLVLLYSRSFEWLVVAFAVRGLKEFGE
                     PTRKSLIMDLAPDHNKAAMFGLYYLIRDVVVSAAALGGAYLWNVGPEVNLLAAFAFGV
                     IGTITFALFGKDARSSAAPRP"
     gene            complement(6630..8258)
                     /locus_tag="VEC56_04680"
     CDS             complement(6630..8258)
                     /locus_tag="VEC56_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13479.1"
                     /translation="MSTGFRLLVAACVSLSVLPTCSEQITPSAPVFSLSSSSCTTDLD
                     CPLGQECVDGTCVPLRRSIFPHVQTGVVLLRAPVGDEIEWCATHYDVMIGAVRPDEMR
                     ALNPSMRFFDYTLSRYHTFDSGTKTAAEWAIARGWDPEDFYLHYEEDVYVPTWEGTVI
                     VPGFPPGMVPGWNPGGGANPASASHRSQSRAVGFRWGNPALPYFANVSHPGYRLYFLA
                     RTTGLVDGSWYFNQPFATGPLEGVLCDEAVYYPAFGEGLLDRTVEYYGIPLTDNHPYA
                     VAVAGFYPALAAALRNTVGTTVDVMPNFGHVLFLNYPNPSAMTIQTTTPWILGEVWVT
                     FTGLSSPTSGPNRCITYENDYVNAVRDIVEQTRARGRRVLGARDLSNGTSGSDRGRVF
                     TLSLFYLLYNPNSYYVYETVDQSSAHVSAWSWNPLVEFDVGRPATIPPGAVDFEGRAN
                     TREHWVLDSGPDPYRPDLTYRVLARRFTNALVLVKMLPMGSVDDSRSVTTHPLDRGYR
                     VLQADGTLGLSVTEARLRNNEALILVPDVATGVD"
     gene            complement(8364..>8694)
                     /locus_tag="VEC56_04685"
     CDS             complement(8364..>8694)
                     /locus_tag="VEC56_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV13480.1"
                     /translation="IDHVNHLRKEGMPMERAILEGGRERLRPILMTALTTILGLIPMA
                     IGGSHIGEAQYYPLARAVMGGLVSSTFLTLLVLPTYYILGERAKAWWLGVLARSRPGT
                     SSTRVLD"
BASE COUNT         1461 a         2826 c         2867 g         1540 t
ORIGIN      
        1 tatgagacgc tcgatgcact cgatccatct ggttccctcg accgcggggc gctcgatcgc
       61 catcgccacg ctcctggcgc tcgcgctgat cggcatcgcc gtgagcgcgc gcgcgcagac
      121 cgccaacacc atccaggtgc gcgggctggt cgacctggtc gcgtccgatg tcggcgacta
      181 tcgctatctc aacaccaaca acaccaacga cggcaacttc gacgcgctgc gctcgcgcct
      241 gttcgtcgac ggcaagcgcg gcaacacctc cgtcttcctc cagttcttga tctcgccgga
      301 gagctacaac gagttccgct tctacggcgg ttacgtcatg cagcgcattc acgaggagcg
      361 caacgtcttc atcgaagcag ggctcatccc ggtgcacgac ggcatctggg ccagccacac
      421 ctattcgaac aagaacccgc tgatggggat cccgctcatg cagtactgga agtcgagcct
      481 cccctacacg atgatgccca acgacatcga cgacctcctc tcgcagcgcg ggcgggggca
      541 ggaatacggc gtctcctacg ccgactccaa cggcacgcgc ggctatcgct acgcctccat
      601 gccgatcctg tacgacaatt gctggaacta cggcgcgtac acgctgggca cgctcgggaa
      661 gctggagttc tcgctcggta tcacgctggg agcgccgtcg gccgccgtgc agggcacgga
      721 cttcaacgac aacatcgcga cccacgccaa gttcggcttc gcgttcacgc cggggttcaa
      781 ggtatgggga tcgtacgcgc acggcgcgta tttggaccgc gcggtggcgc cctacctgcc
      841 gttgggcaag accgtcaacg actactacca gaatctgtac ggcgggagcg tcgattggaa
      901 ggtggggaag ttctggttca tgagcgagta cttctacaac cactacgaca cgcccatccg
      961 caacgacggc ttgagcaacc agtcgctcta cgttcaaacg gtgttctcga tggcggcggc
     1021 gtgggacctg tgcctgcgct gggacgcgct tcgcttcgga gaggtccagg acagcgcggg
     1081 caacacgatg acctgggacg agaacgtgta ccgcgtcgag tcgggcgtgg gctaccacgt
     1141 ctcgcgcgac ctggtggtca aggcggtggc ccagatgacg cgcttcgaag gcgactggga
     1201 cctgattccc gccatccagg cgtcgttcgg cttctagggt ggaaatggcg tccgtacgcc
     1261 ctgtatactg atatggccgc gccggcgcgg ggccggaaca atgagtccgg tcttgacccg
     1321 ggtacccacg ctgcgacgat gtgtggtgtg gtccgcgccc ggcgcggcgc tagactcgat
     1381 ccccgaaggt tcgcttcaat ccgtccatgg tacccacgcg cggcgaccat ccgagcacgt
     1441 cgcgcgcctt ggtcacatcg aacggccaca gcggctcgag cgagcggatc ttgtagcgcg
     1501 ttagcggcac atcgcgcttc atgagcttgc cgaggatctc gatcttccat cccagcacca
     1561 tgagggccca caccggcacg cggtgcacgc gcagggactg cgcgcggccc gcgtatgcga
     1621 gatagtcgtt ctgcgtgatc ggggtcgggt cgaccacatt taccacgtga cccagcgcac
     1681 gcggagagct ctcgctcgcc aacagtgcgt ccaccacgtc gtcgcgatag acgagcggca
     1741 ggttgcggtc gcccttgccc gcgacgatcc agcgtcccgc gatgccgatg acaccgttgg
     1801 gggtgacgtg ctcggcgccc gggccgaaaa tctgcccggg cctcacgatc acggctcgca
     1861 gccccttgtc acgaatcgct tccagcacca tctgctcggc ccgaaacttg gtctgcgtgt
     1921 atacgccgcg ccggtcggga tgcggttcgt acggcgacga ctcggtcacg gggacgccgg
     1981 tggtatgccc cgcgtggtcg gccacactca tcgagctcac gtagacgagt ttggcgccgt
     2041 ggcgcaggca cgcgtcgatc acgttgcgcg ttccccacac ggtcccctgc tcgaactctt
     2101 ccttgccgcc cttcatcgca gcccccacgt ggtacacgac ctcgacgccc tgcaccgcgc
     2161 gctcgaccac ttcgggttgt cccaagcttc ccgccacgac gtcgtcgccg gcgcccgcgg
     2221 gtgcgcgccg caagagcagc cgcaccgatt cgccgctctc ccgcaggcgc tccaccagcg
     2281 cgcgccccag gaaaccgccg ccgccggtga cgaggacgcg cgagcgagcg agcggcttgg
     2341 tcatctcgtg cgagagtcgc accgcctttt gatggtcggc gtcgcggctc gcaacatcga
     2401 tccacttcgc catgcgccgg ccttcttccg ccggcaccgg cacgggcgtg ccgttgtgca
     2461 gtgccaggtg gaagtcctgc acgcagcgat agatccccgg cgagggcttg agcgacccgg
     2521 tcatgaagcg gagcaggttc aacggaatga cgaacgcgcg gcgggccgcg ttgcgcgcgc
     2581 cgttgaggat attgccgatc tgcttcggcc ccgggagcac gcggttgacg tcgcacacct
     2641 gcaggaagcg atcgaccttg atcaccccgc gcgtgccgtg aatctccaat tcgctctgga
     2701 tgggccgcgt gttccacgag aggtacgcgt agccggtgcc gtgctcgcac tccgcgtacg
     2761 cgcgccactc gtcgaaggtc agcatcgggt tgcggcccga ttcgtagaag ttgacgtcga
     2821 gggtgcggac ctggcccacg aagctttcga tgagatacag gctgtgcacg ccgagatcgc
     2881 ggaagggata cgacccttgc cgatagggct gtggcaaggg tccgcccgcg tacgtcgggt
     2941 agtccgaact tcgaatcgaa tgaaccgcga gcacgtcgcc gatggagccc gagcgcgcaa
     3001 ccgcgatcgc cttcaagacc acggggtcga agcggtccga atgattgatc gagagcacca
     3061 cacccttctc gcgcgcgtgc gcgatcatgc ggtcgcattc ctcgaccgat tccgccatcg
     3121 gcttctcgac gaagacgtgg cagcccatgt ccatggcggc gagcgacagc gagcagtggc
     3181 tttcgggagg cgtgaggata tgaatgacgt ccggcttggc gctggtcatc gacgagaggt
     3241 ccgtgtacac cgcgcgcacg cggaacttct tggccagcgc ctccgcgcgg gtgcggtcgc
     3301 ggtcgcagat gccaaccacc tcgacgaatg gcagcgcgtt caacgcgcgc aggtggtgcg
     3361 ttgcgacgta gccggcgccg accaagccga cgcgaacttt gggcgcgatg ggactgctca
     3421 tttatatagg aagactaatg ggccgactgg ggttgcaggt actcctgaac gtaccggcga
     3481 acgccgtcct cgacttgcaa gaatggcgcc gaatagccgg ccgcgcgcag cttcgtcatg
     3541 tcggcctggg tgtggtactg gtacttctct cgtatcgact ccggcatatc gatgaagcgg
     3601 atgcggggcg ggagccccac ggcggaaaag agcgccttcg ccaggtcgac ccacgtccgc
     3661 gcgctgcccg tgccgcagtt gaagagcccc gagacctccg ggcgatcgag gaagaatagc
     3721 gtcaccgcga cggcgtcgtc cacgtgaatg aagtcgcgcc gctgctcccc gtccttgaac
     3781 gcgggccggt aggacttgaa gagcgcgatc tcgccctctc gcatcacctg cgcgtaggcc
     3841 ttgttgacca acgagcgcat gtcgcccttg tggtcttcgt acgggccgta gacattgaaa
     3901 tacttgatcc ccgcgacccg cttcaagacg ccgttcctca aggcccactc gtcgaaccac
     3961 tgcttcgagg cgccgtacag attgagcggt tttaacgtcg gtgtcaccgc atcctcgtcg
     4021 gagtatccca atgaaccgtc gccgtaggtg gcggcactcg acgcgtagac gaagcgcgcc
     4081 ccgccggcga gcgaccactc gcacacctcg cgcgtgtact ccgtgttgtt gtcgcgaaga
     4141 tagccttcgt cggtctcggt ggtcgagctg cacgcgccca ggtggaagac cgcggaggcc
     4201 ttgggggctt tgcccgcgcg cacgcgcgcg cgaaactcgc gcttgtcgag gtattcgccg
     4261 atttgcagtc gttcgaggtt gcgaagcttc ccgggatcgc cctcgacgtg atcgaccacg
     4321 accacgtcgt cgtgcccgcg tgcgttcaac gcgtcgacga ggttgctgcc gatgaacccg
     4381 gctccgccgg tgacgatgat gggtgctgcc acggggcgat tatagggcac cgggcgcggg
     4441 gtggccagcg ggctatccct tcgccaccgc gcgctcggcc cacgccagcg cgtagtccgc
     4501 cacctcttcg aaacccgggc cggcgatgat gctgtagtgg ctgtgccctg ggaactcctt
     4561 gaagtcggtg accccggccg accctttata gcgcttgtag ttcgtgcggt tcagcgccgc
     4621 cggcatgatg tgatccgcgc tgcccgcgat caggagcagg ggcgcgcgct tccgcgcgaa
     4681 gtccacgtgc gcggccgacg agagaccgcc cgacgcgagc atgcgcgact ccgggacgac
     4741 gtggcgatcg tacgcctcgc gctgcgcggc gggcggcatg ttgttcacga aggtgtactg
     4801 gaagtgttcg aacggcatga ggtacggctg gtgtcgcttg atcgggttga tggccgggaa
     4861 gaccgagcgc acgaacgacc actgcgtgcc gaggacaccc tggggaggga ccgagtcgat
     4921 ggccacgccc gcggcgccaa cgtcgcgctg catcaggagc tgcgtgagca gaccgccgaa
     4981 cgagtgcccc atgatgatgg gcttctcgtc cagcgactgg atggtgcgca cgtggtggtc
     5041 gatcacctcg ggcagcgtga ggcgcgcgag cgcagggtcg gggtgcttgc cgcgcaacga
     5101 ttccacggtg tcatcgcgca atggatacgc gatgggcacg cacttgtatc cccgtgcctc
     5161 gaagcgctga acgaacggat cccagcatcg cttgctgacg aagttcccgt gcacgaaggc
     5221 gatggtcttg gtggacatga ttccctcctg gttgagcctc gctctgcacg aggtcattcg
     5281 gtgattatac ataccgaggc gagaacgcgc accccgaagc cgcacgcccg accgttcgat
     5341 gcgaactctt tacaattccc ggtccgcgtg cattagactc ccccgcgtcc catgaacgcc
     5401 cgcctctccc gcatctccca gtttctcgcc ctgcggcgca gcaccgtggg catgttcgcg
     5461 atggtgctct tggtgggcat gggcgaggag atggcggagc gatttctccc catctatctc
     5521 ctcgcggtcg gggggagtgc catgtccatc ggcgtgttgc aggcgatgga caacttcctc
     5581 tccgcgctct actcgttccc gggcgggtac ctggcggacc ggatcggcac gcgcaaggca
     5641 ctgctcgttt tcaatctgat ggcgatggcg ggtttcgcgg tcgtgatcct gattcccacc
     5701 tggcaggccg tgctggcggg agccgtactg ttcatctcgt ggtccgcgat ctccgccccc
     5761 gccaccttga gtttgatcta caaggttcta ccacccgaca agcgcacgat gggggtgagc
     5821 atggtgtcaa tcatgcggcg catcccgaag gggctggggc cgctgatggg cgggttgtgc
     5881 atcgcgctct ggggcgagcg cgagggcgtg cggctcgcgt tcatcgcggc cttcgtgctg
     5941 gccctgttcg cggtcgtctt gcagcggcgt ctgatcgcgg acgatggccc ccgcgcgcgc
     6001 gccgcggccg gtctcgcgct ccccttggtc gagaaggacc cgcgccggat gttccgtctc
     6061 atgagcccgg agatgcggcg gctccttttc accgatattc tggtgcgttt ctgcgagcag
     6121 attccctacg ccttcgtggt cgtgtggtgc atgaaatcga ttccccatcc ggtgacggcg
     6181 gtccagttcg gcgtgctgtc gaccatcgag acggtcgtgg cgttgctctg ctacatcccg
     6241 gtcgcgcacc tagccgatcg cggccacaag aagcgctacg tgctgatcac attcggcttc
     6301 ttcgccctgt tcccgctcgt gctcctgtac agccgctcgt tcgagtggct cgtcgtggcg
     6361 ttcgcggtcc gaggcttgaa agagttcggc gaaccgacgc gcaagtctct catcatggac
     6421 ttggcgccag atcacaacaa agcggcgatg ttcggcttgt attacttgat ccgggacgtc
     6481 gtcgtgtccg cggcggccct gggcggggcc tacttgtgga acgtgggccc ggaagtcaat
     6541 ttgctcgccg ccttcgcgtt cggcgtgatc gggacgatta cgttcgcgct cttcggcaag
     6601 gacgcgcgat cgtccgccgc gccgcgtccc tagtcgaccc ccgtcgccac gtcgggtacc
     6661 aagatcagcg cctcgttatt gcgcaagcgc gcctcggtga ccgacaaccc gagcgtgccg
     6721 tcggcctgca ggacccgata gccgcggtcc agcgggtgcg tcgtgacgct tcggctgtcg
     6781 tcgacggagc ccatcgggag catcttcacg agcaccaggg cgttggtgaa gcggcgcgcg
     6841 aggacgcgat acgtgaggtc ggggcgatag gggtcgggac ccgagtccaa gacccagtgt
     6901 tccctcgtgt tggcccgtcc ctcgaaatcg accgcgcccg gcgggatggt ggccggccgc
     6961 ccgacgtcga actcgaccag cgggttccag gaccacgctg acacgtgggc gctcgactgg
     7021 tccacggttt cgtagacgta gtacgagttg gggttgtaga gcagatagaa aagcgacagc
     7081 gtgaagaccc gtccgcggtc cgatcccgac gtcccgttcg atagatcacg cgctccgagc
     7141 acgcgccggc cgcgtgcgcg ggtttgctcc acgatgtcgc gcacggcgtt gacgtagtcg
     7201 ttctcatagg tgatgcaccg gttgggtccg ctggtcggcg acgagaggcc ggtgaaggtc
     7261 acccacacct cgcccagaat ccacggcgtg gtggtctgga tggtcatggc cgatgggttg
     7321 ggatagttca agaagagcac gtgcccgaag ttcggcatca cgtccaccgt ggtgccgacc
     7381 gtgttccgca gggccgcggc cagcgccgga taaaaacccg cgacggcgac ggcgtagggg
     7441 tgattgtcgg tcaacggaat cccgtagtac tcgacggtac gatcgagaag gccttctcca
     7501 aaggcggggt aatacaccgc ctcgtcgcat aggacgccct ccagaggacc ggtcgcgaac
     7561 ggctggttga agtaccagct gccgtccacc agtcccgtcg tgcgcgccaa gaagtacaac
     7621 cggtaaccgg gatgagacac gttggcgaag tacgggagtg cggggttgcc ccaccggaat
     7681 ccgaccgcgc gggattggga gcgatgcgac gccgaggccg ggttggcccc gccgcccggg
     7741 ttccagcccg gtaccatccc cggcggaaag cccggcacga tcaccgtccc ctcccacgtg
     7801 gggacataca cgtcctcttc gtaatggaga tagaagtcct cggggtccca cccgcgagcg
     7861 atggcccatt cggccgcggt cttggtcccg ctgtcgaacg tgtggtagcg cgagagcgtg
     7921 tagtcgaaga agcgcatgct cggattgagc gcgcgcatct cgtccgggcg gaccgctcca
     7981 atcatgacgt cgtagtgcgt cgcgcaccac tcgatctcgt cgccgaccgg cgcgcgcaag
     8041 aggaccacgc ccgtctgcac gtgcggaaag atcgagcgcc gcagggggac gcacgtcccg
     8101 tcgacacatt cttgtcccag cgggcagtcg aggtcggtgg tgcaggacga actggagagc
     8161 gagaacacgg gtgccgaggg cgtgatctgc tcggagcagg tcggcagcac ggagagagac
     8221 acgcacgccg ccacgagcag gcggaaacca gtggacacgg taatgctccc ggggccaata
     8281 ggccgattgg tgacggggga gcgtcagcgg aagatgacta caacgaggcg ggatgcggag
     8341 ccgaattcag gccgggtgtg taatcaatct aacacgcggg tcgagctcgt tcccggccgc
     8401 gaacgcgcca acacgcccag ccaccacgcc ttcgcgcgct cgccgaggat gtagtacgtc
     8461 ggcaggacca ggagggtgag gaaggtcgag ctcaccagtc cgcccatgac cgcgcgcgcg
     8521 agcgggtagt actgcgcctc gccgatgtgc gaaccgccga tggccatggg aatgaggccg
     8581 aggatggtgg tgagcgcggt catcaagatg ggtcgaagcc gctcgcgccc gccttccagg
     8641 atggcccgct ccatcggcat gccttccttg cgcagatgat tgacgtggtc gatc
//
LOCUS       DASPGK010000163         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_745039, whole genome shotgun sequence.
ACCESSION   DASPGK010000163 DASPGK010000000
VERSION     DASPGK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745039"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VEC56_04690"
     CDS             <1..204
                     /locus_tag="VEC56_04690"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXV13481.1"
                     /translation="KAVQKLADRLNIPHYASLENRMGCALGACRACVVPTKLDGASPY
                     RTVCHDGPVFDASVLQWDALPVP"
     gene            329..1243
                     /locus_tag="VEC56_04695"
     CDS             329..1243
                     /locus_tag="VEC56_04695"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXV13482.1"
                     /translation="MNDISVQIGPVRFKNPVFIASGICGYAEEYSPLVNLSRLGGVVT
                     KTITMLPRAGNLPPRIHELPHGMLNSIGLENVGLDRYVNEKLPQLNQRPDVGVMVSVA
                     AETEDEFIRLAERFAPMEGYAGLELNLSCPNVKGEALDQGRNPQLVETISRIVKERIP
                     KKSLWVKVTPNITDVGTVARAAEQGGADAIAAINTLVGIDFDLSSGKPVFARGSAGYS
                     GPAILPIALQKVWECAKAVSIPIVGIGGITTIDDARKFFLAGATAIQVGTSLYNDPGL
                     PERIVDALAAHPEWIHARARLTPSLPNG"
     gene            1306..2817
                     /locus_tag="VEC56_04700"
     CDS             1306..2817
                     /locus_tag="VEC56_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010382062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HXV13483.1"
                     /translation="MSGAASTPGTGALPAFKEVLGHPRPLWMLFMSEFWERFAFYGMR
                     WALVLYMVAQFHGGDPSGEAPSSRIYGAYLALVYAAAIFGGYAADRILGYQRSILLGA
                     IIMATGLFMVSMPNEQIFKLGLATIIAGNGLFKPNISTMVGKLYAPGDQRRDSGFTIF
                     YMGINAGAFIAPILTGWFADRVFGTPDMPAYKVVFITTGIGMLISMVWFWFGRKQLQG
                     IGRPPEADGGGRRKVLYVGLGALAAIPIVYFLLSVGADKLQWLLTAMFIVLCVMIMRE
                     GMREGQVQRDRAIAMLVIFAFNILFWMFFEQAGSSFTFLADQIVDRDLGAAGGFIFPN
                     AWFQSVNAIGIVVLAPIFAWLWVKMGKMNPSIPRKFGLGLIFNGLAFVVLMVALSSLV
                     DAAGKIPFWTLFTVYLIQTVGELCLSPIGLSMVTKLAPMRLVGFGMGGWFLSTGIGNN
                     LSGIFAGHVSGDTGMTTASALSGYTFGFWALVIPGVLLFLIAPLIQRLMHGVK"
     gene            2843..>3303
                     /locus_tag="VEC56_04705"
     CDS             2843..>3303
                     /locus_tag="VEC56_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13484.1"
                     /translation="METLTSYWHARRTLRRTTALVACAALVLTACDSFRRVERRSIAP
                     DAFRAMGAGEKQELREASPFLKAHMRNGDLFVLQDWIAGGDGVSGSGTRYDPSRNPVE
                     TGQFHLAVDSVAVFETNVTDSSPALAAITILAVATAAVAIMCAINPKSCFGS"
BASE COUNT          541 a         1092 c         1017 g          653 t
ORIGIN      
        1 aaggccgtcc agaagctcgc ggatagactc aacatcccgc actacgcctc actcgagaat
       61 cgaatgggct gcgcgctggg cgcctgccgc gcgtgcgtcg tccccaccaa gctcgacggc
      121 gccagcccat accgcaccgt ctgccacgac gggcccgtct tcgacgcgag tgtgctgcag
      181 tgggatgcac tgccggtgcc atgatgaacc tcgcgcgggt cctgtcgtcc aacagcccac
      241 tgcgctcggg cctaccgcgc atgcgcacta gtagtccctc gcttgtgggc gcccgttgta
      301 cgccacccgc gctcgaggta ccacggtcat gaacgacatc tccgtccaaa tcggtcccgt
      361 tcgcttcaag aacccggtgt tcatcgcgtc ggggatttgc ggctatgccg aggagtactc
      421 gccgctcgtc aacctctcgc gtctgggcgg tgtggtgacg aagaccatca ccatgctgcc
      481 gcgcgccggg aatctcccgc cgcgcattca cgagcttccg cacggcatgt tgaactcgat
      541 cggcttggag aacgtcggcc tcgatcgcta tgtgaacgag aagctcccgc agctgaacca
      601 gcgtcccgac gtcggcgtga tggtgagtgt cgcggccgag accgaggacg agttcatccg
      661 gctcgcggag agattcgcgc ccatggaggg ctacgcggga ctcgagttga acctctcgtg
      721 ccccaacgtc aaaggcgaag ccctcgacca ggggcgcaac ccgcaattgg tcgagacgat
      781 ctcgcgcatc gtgaaagaac gcatccctaa gaagtctctg tgggtgaaag tgacgcccaa
      841 catcaccgat gtcggcacgg ttgcgcgcgc ggccgagcaa ggcggcgccg acgccatcgc
      901 cgccatcaac accctggtcg ggattgattt tgatttgagc tccggcaaac ctgttttcgc
      961 gcgcgggagt gcgggctatt ccggccccgc cattctcccc atcgcgctcc agaaggtgtg
     1021 ggagtgtgcg aaggcggtgt cgatccccat cgtcggcatc ggtggcatca ccaccatcga
     1081 cgacgcccgc aagttcttcc tcgccggggc gaccgcgatc caagtgggga cgtcgctata
     1141 taatgacccc ggtctccccg agcgcatcgt cgacgcgctc gccgcgcacc ccgagtggat
     1201 acacgcccgg gctcgcttga cgccttcttt gccaaacggt tagactcccc cgacggttcg
     1261 atcgcaactc ccccacatgt atcgttgcat ggaaggagaa accgcatgag cggtgccgct
     1321 tcgacgcccg gaacgggcgc actccccgcg ttcaaagagg tcctcgggca tccgcgcccc
     1381 ttgtggatgc tgttcatgtc cgagttctgg gagcgcttcg cgttctatgg aatgcgatgg
     1441 gcgctggtgc tgtacatggt ggcgcagttc cacggcggcg atccgtcggg cgaagcgccg
     1501 tcgagccgca tctacggcgc ctacctcgcg ctcgtgtacg cggccgccat cttcggcggc
     1561 tacgcggccg atcgcatcct cggctaccag cgctcaatct tgttgggcgc gatcatcatg
     1621 gccaccgggt tgttcatggt ctcgatgccc aacgagcaga ttttcaagct cggactcgcc
     1681 accatcatcg ccggcaacgg gctcttcaaa cccaacatct cgaccatggt gggtaaactg
     1741 tacgcaccgg gcgatcagcg ccgcgactcc ggcttcacca tcttctacat gggcatcaac
     1801 gcgggtgcgt tcatcgcgcc catcttgacc gggtggttcg cggaccgcgt gttcggcacg
     1861 ccggacatgc cggcctacaa ggtcgtgttc atcaccaccg gcatcggcat gctgatcagc
     1921 atggtctggt tctggttcgg ccgcaagcaa ctccaaggca tcggtcggcc gcccgaagcg
     1981 gacggcggcg gacgccgcaa ggtgctgtac gtcggcttgg gtgccctggc cgcaatcccg
     2041 atcgtgtact tcctcctgtc ggtcggcgcc gacaagctgc aatggctcct gaccgcgatg
     2101 ttcatcgtgc tgtgcgtgat gatcatgcgc gaaggaatgc gcgagggtca ggtccagcgc
     2161 gatcgcgcca tcgcgatgct ggtcatcttc gccttcaata ttctgttttg gatgttcttc
     2221 gagcaggccg gcagctcgtt cacgttcctc gcggaccaga tcgtcgaccg cgacttgggc
     2281 gctgccggcg gcttcatatt ccccaacgcg tggttccagt ccgtcaacgc catcgggatc
     2341 gtcgtgctcg cgcccatctt cgcgtggttg tgggtgaaga tggggaagat gaacccctcc
     2401 atcccgcgca agttcggact cggtttgatt ttcaacggcc tcgccttcgt ggtcctgatg
     2461 gtcgcgctct cgagcctggt ggacgcggcg ggcaagattc cgttctggac cctgttcacg
     2521 gtctatctca ttcaaaccgt cggcgagctg tgcctgtcgc ccatcggcct ctcgatggtg
     2581 accaagctcg cgcccatgcg cctggtcggc ttcggcatgg gcggctggtt cctctcgacc
     2641 ggcatcggca acaacttgtc aggcatcttc gccggccacg tcagcggcga caccggcatg
     2701 acgaccgcgt cggcgctgtc gggctacacc ttcggcttct gggccctggt catcccgggc
     2761 gtcttgttgt tcttgatcgc gccgctgatt cagagactga tgcacggggt gaaatagcga
     2821 atcgctcctt tcgaagggag tgatggaaac gctcacttcg tactggcacg cgcgacggac
     2881 tctgcgacgc acgaccgcgc tggtcgcatg cgccgcgctc gtgctgaccg cgtgcgattc
     2941 gtttcggcgc gtcgagcgac gaagcattgc gcccgacgcg tttcgcgcca tgggcgcggg
     3001 tgagaagcaa gagttgcgcg aagcatcgcc gttcctcaag gctcacatga ggaacggcga
     3061 tctttttgtg ctgcaagact ggatcgccgg tggcgacggt gtctcgggca gcggcacgcg
     3121 ttacgatccg tcacgcaatc ccgtcgagac cggacaattc cacctcgcgg tcgactcggt
     3181 ggccgtcttc gagaccaacg tcacggactc gtcgccggcg ctggccgcca tcaccattct
     3241 ggcggtggcc acggcggccg tggccatcat gtgcgcgatc aatcccaagt cgtgcttcgg
     3301 gtc
//
LOCUS       DASPGK010000164         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1214271, whole genome shotgun sequence.
ACCESSION   DASPGK010000164 DASPGK010000000
VERSION     DASPGK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214271"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VEC56_04710"
     CDS             <1..921
                     /locus_tag="VEC56_04710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV13485.1"
                     /translation="GRSKANTILITGESGSGKELVARAIHACSHDDPRPFMEINCASV
                     PETLLESELFGYEKGAFTDAKTRKKGLVELAEGGTLFLDEIGEMGIMLQSRLLRVLEN
                     KTFRRVGGVKDLTVNTRIVSATNRDLASAIKDKLFREDLYYRLKVIPLHIPPLRERRE
                     DIPLLVSHFIERFNRELGKQVPIPSSDVMELLVRYDWPGNVREMRNVIERALLLDAQD
                     ELLVAHLPAEIRGQAGAELATVRAPAAAVTGAFFPKTLRDMERIQIERTLEETRGNKS
                     RAAAILGISRQTLREKLKSFHGDEGVRDEA"
     gene            1137..1949
                     /locus_tag="VEC56_04715"
     CDS             1137..1949
                     /locus_tag="VEC56_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV13486.1"
                     /translation="MPFHRGVSDHPSHFSTESARAALRRTLADRLGRMGVRPAPELAP
                     SELQRLGDVEALVAQCVRAARHTAGSVRAYASLIADGYAHEANASTWAAKIERSAMDL
                     DDFATRFGALRLCASERPSRVEWGEVLNRVAARCAGLGACTIEANDRTDGPFRQRAEL
                     LGRALFQVVRNAVEAAPRGGSVRARVDECRVGTTRVFHVRVADDGPGVSGVREPDDAW
                     RPFVTTKPDHAGLGLAFVAACAPVLGAVAGMRRESDRTVVHVLVAEEGDLEW"
     gene            1943..2428
                     /locus_tag="VEC56_04720"
     CDS             1943..2428
                     /locus_tag="VEC56_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV13487.1"
                     /translation="MVVLMVGIPGVDAETLPRSNGNLEFVAVESLAEALAFLDGHACE
                     AVMIGPNAADMANEPLSALGLRGGAMERAMRGGSASIETRADSGDGPRERGDVVESVF
                     RRGSIRDMERLMILDRLARLNQNRTRSAESLAISVRTLRNKLRDYRQATTLVTADSET
                     G"
     gene            2473..3969
                     /locus_tag="VEC56_04725"
     CDS             2473..3969
                     /locus_tag="VEC56_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV13488.1"
                     /translation="MSSQQVHYIVLSLDATIRQALSGLFPREGWRVDTVDDPSGVVEA
                     LTETTAGVLIDEASEPAYVALVKRLRKTAPAIDAAVAGGPKAEAVKAGERLEGIDLYI
                     ERPLDASALRATLDHRFALIALKSRAGIIGRSPAMEELLESVLLVAPTEVPILIQGES
                     GTGKDVVARAIHDASRRRDRPFEAINCGSLAEGVLESELFGHEKGAFTGAVARRAGMF
                     ERASTGTIFLDEVGETSPNMQVRLLRVLETGDVLRVGGGTPLKVDVRVIAATNRNLAE
                     AVRSGAFRQDLYYRLKGVSLFLPPLRERRDDIPILVQRFIAQANRAHQKSVRGVEADA
                     MRRLVDHAWPGNIRELKNLVETLVVLTPGPRITRALVDAHLSDAASAAASPLLPVPLG
                     RSREEAEREMLYASILALHRDVREILHVLKEGPLRAAPEAGALGGLREVRAEDGSHAP
                     PTLSLDRIERTAVKEALNRSGGNRRRAAESLGISERTLYRKIKEYGLA"
     gene            4110..5126
                     /locus_tag="VEC56_04730"
     CDS             4110..5126
                     /locus_tag="VEC56_04730"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HXV13489.1"
                     /translation="MHVRTVGLCALGLFVAAPVHATKLAGEFMAPGGGARALGMGGAF
                     AAVAADASAVFWNPAGIAGMEKRQVLAMHAEQFGDLLNYNFASYVQPTGLVDEARKPA
                     FGFGLIHLGDPDQLLTSQYRSVDNDSDGEIDYLIDEDGNVVDYDAIPRESNNSFAGLG
                     TFALETSAGSVGGTLKLIYQDFIAGESSMGIGIDLGFLRRDFLTENLSVGVKLADATG
                     TYISWSTGTNEFIVPSLKLGSAYRIESASLNGSVLFAVDADLFFDDRQTASQFWSETV
                     STDLRLGVELSFQEKVMLRGGLDAENPTAGAGLYFGVFGLDYAYLHHDAFDSTHRVSL
                     LASF"
     gene            complement(5140..>5524)
                     /locus_tag="VEC56_04735"
     CDS             complement(5140..>5524)
                     /locus_tag="VEC56_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV13490.1"
                     /translation="YRVKSGDTLSEIAQRFGTSVSRIRSWNNLSRRRHIYAGQRLTIY
                     TNRAATTEVASPAPASAPTPGDDVTKLTHVVVRGESLFSISRQYNVGVQDLMAWNNRG
                     SSMIKAGERLVVYKRGTTPSENGAR"
BASE COUNT          901 a         1838 c         1880 g          905 t
ORIGIN      
        1 ggccgcagca aggcgaacac gatcctgatt accggcgaga gcggttcggg caaggagttg
       61 gtcgcgcgcg ccattcacgc ctgcagccac gatgacccgc ggcccttcat ggagatcaac
      121 tgcgcatcag tccccgagac gctgctcgag agcgagctgt tcggctacga gaagggggcc
      181 ttcaccgacg ccaagacgcg caagaagggg ctggtcgagc tggccgaagg cggcacgctg
      241 ttcctggacg agatcggcga gatgggcatc atgctccagt cgcgcctgct gcgcgtgctc
      301 gagaacaaga ccttccggcg cgtgggcggg gtcaaggact tgacggtcaa cacgcgcatc
      361 gtgtcggcca ccaaccgcga cctagcctcc gcgatcaagg acaagctctt ccgcgaggat
      421 ctctactacc ggctcaaggt gatcccgctc cacattccgc cgctgcgcga gcgccgcgaa
      481 gacatcccgc tgctcgtcag ccacttcatc gagcgcttca accgcgagct cgggaagcag
      541 gtgccgatcc catcgtcgga cgtgatggag ttgctcgtgc gctacgattg gccgggcaac
      601 gtgcgagaga tgcgcaacgt gatcgagcgc gcactcttgc tcgacgccca agacgagctc
      661 ctcgttgcac acctgccggc cgagattcgc ggccaagcgg gcgcggagct cgcaaccgtg
      721 cgcgccccgg ccgcggcggt gacgggcgcg ttcttcccca agacgctgcg cgacatggaa
      781 cgcatccaga tcgagcgcac cttggaagag acgcgcggca acaagtcgcg cgcggccgcg
      841 atcctgggca tctcgcggca aacgctgcgc gagaagctca agtcgttcca cggggacgaa
      901 ggcgtgcgcg acgaggcgta gatccgccgc cgccaccggg cgagcgccga gggcttcctt
      961 cgatgctcgc ccgacccggc cccccggtgg ccagttccgt ttccctgcgc atcccgcgcg
     1021 cgaccgaacc ggggtatttc ttgccgcctc ggtcgccggg cgagctagcc tactcgcgta
     1081 agtaactaga tacccaccaa caggatagtc cggttcccgg agtcggggca cggcgcttgc
     1141 catttcaccg cggcgtgtcc gaccacccgt cgcacttctc gaccgagtcc gcgcgcgccg
     1201 cactgcgtcg cacgctggct gatcggctcg gccgtatggg cgttcgaccc gcgcccgagt
     1261 tggccccctc ggagctgcag cgcttgggcg acgtcgaggc gttggtcgca cagtgcgtgc
     1321 gcgcggcgcg tcacaccgcc ggcagcgtgc gcgcgtacgc gtcgctgatc gccgacggct
     1381 acgcgcacga agccaacgcg tccacgtggg cggcgaaaat cgagcgctcg gcgatggacc
     1441 tggacgattt cgcgacccgc ttcggcgcgc tgcgcctgtg cgcgagtgag cgtccgtcgc
     1501 gcgtcgagtg gggcgaggta ctgaatcgag ttgcagcccg ctgtgcgggt ctgggtgcgt
     1561 gcacgatcga agcgaacgat cggaccgacg gtccgtttcg acagcgtgcg gagcttttgg
     1621 ggagggccct gtttcaagtc gtgcgtaacg cggtggaggc cgcgccccgc ggggggagtg
     1681 tgcgcgcgcg cgtagacgag tgccgcgtgg gaacgacgcg cgtgtttcac gtgcgcgtcg
     1741 ccgacgacgg acccggagtg tcaggcgtgc gcgagcccga cgacgcgtgg cgcccgttcg
     1801 tgacgaccaa gcccgaccac gccggtctgg gcctcgcgtt cgtcgcggcg tgcgcaccgg
     1861 tcttgggagc ggtcgcggga atgcgccgcg agtccgatcg aacggttgtt cacgtgctgg
     1921 tcgcggagga aggagacctc gaatggtagt gctcatggtg ggaattcccg gcgttgacgc
     1981 cgagacgctt ccccggtcca atggaaatct cgagttcgta gcggtcgaga gcctcgcgga
     2041 ggcgctggcg ttcctcgacg gacacgcgtg cgaagccgtc atgatcgggc ccaacgcggc
     2101 cgacatggcc aacgaaccgc tgtccgcgct cggtttgcgc ggcggcgcca tggagcgcgc
     2161 gatgcgcggc gggtccgcct cgatcgaaac gcgcgcggac tccggcgacg gaccgcgcga
     2221 gcgcggtgac gtggtcgaga gcgtgtttcg ccgcgggtca atccgcgaca tggagcgcct
     2281 gatgatcctg gaccgcctgg cacggctgaa ccagaatcgc acgcgctcgg ccgagtcgct
     2341 cgcgatcagc gtgcgcacgc tgcgcaacaa gctgcgcgac tatcgccagg cgaccacgct
     2401 cgtgaccgcc gactccgaaa cgggttgaca gtctggcagt cgctgggcta gcctcacctg
     2461 cggtgaaacc gcttgagttc acagcaggta cactacatcg ttctctcgct cgatgcgaca
     2521 atccgccagg ccctctcggg actcttcccg cgtgagggct ggcgcgtcga tacggtcgat
     2581 gatccctccg gggtggtcga ggcgctgacc gaaaccaccg cgggagtact catcgacgag
     2641 gccagcgagc ccgcctatgt ggcgctcgtc aagcgcctgc gcaagaccgc ccctgcgatc
     2701 gacgccgccg tcgccggggg gcccaaggcg gaggcggtca aggcgggcga gcggctcgag
     2761 ggaatcgacc tctacatcga gcgcccgctc gatgcatccg cgctgcgcgc gaccctcgac
     2821 catcgcttcg cgctgatcgc gctcaagtcg agagcgggaa tcatcgggcg ctcgcccgcg
     2881 atggaggaac tgctcgagtc cgtcctcctg gtggcaccca ccgaagtccc gattctaatt
     2941 caaggcgaga gcggcaccgg caaggacgtt gtcgcacgcg ccattcacga cgcgagccgc
     3001 cgccgcgata gacccttcga agccatcaat tgcggttctc tggccgaggg cgtgctcgag
     3061 agcgagctgt tcgggcacga gaagggtgcc tttaccggtg cggtcgcgcg ccgcgccggc
     3121 atgttcgagc gcgccagtac tggcaccatc ttcctcgacg aagtgggcga gaccagcccc
     3181 aacatgcaag tgcgactctt gcgcgtgctg gaaaccggcg acgttctgcg cgtcggcggg
     3241 ggcaccccgc tcaaggtcga cgtgcgcgtg atcgccgcca ccaaccgtaa cctggccgaa
     3301 gcggtccgct cgggggcgtt tcgccaggac ctctattacc gattgaaggg cgtgagcctg
     3361 ttcctgcccc cgctgcgcga gcgtcgcgac gacattccca tcctggtcca gcgcttcatc
     3421 gcgcaagcca accgcgcgca tcagaagtcg gtgcgcggcg tcgaagccga cgcgatgcga
     3481 cgattggtcg accacgcgtg gcccggcaac attcgcgagc tcaagaacct ggtcgagacg
     3541 ctggtggtgt tgacgccggg accgcgcatc acacgcgcgc tggtcgacgc gcacctctcc
     3601 gacgccgcgt ccgccgccgc gtcgccgctc ttgcccgtcc ccttgggccg cagccgggaa
     3661 gaggccgagc gcgaaatgct gtacgcctcg atactggcgc tgcaccgcga cgttcgcgag
     3721 atcctgcacg tgctcaagga ggggcccctg cgcgccgcgc ccgaggcggg tgccctggga
     3781 ggcctgcgcg aagtgcgcgc ggaagacggg tcccatgccc cccctacgtt gagcctcgac
     3841 cgcatcgagc ggaccgcggt caaagaggcg ctgaaccgat cgggagggaa ccggcgccgc
     3901 gcggcggaaa gtctgggcat ctcggagcgg acgctgtacc ggaagatcaa ggagtacggg
     3961 ctggcctgac gggcccgaaa atgccgattt caggcgttgt cgcggcgtgt ggagtggtcg
     4021 tatacttgtt cgcgaagccc ctcccctgtg atcccgatcc gatgaagcat cgtcgtagtt
     4081 tgccaccccg attcggccag aggattcgta tgcatgttcg caccgtcggt ctgtgtgcat
     4141 tgggattgtt cgtcgcggct ccggtccacg ccaccaagct ggcgggcgag ttcatggcac
     4201 cgggcggggg agcccgtgcc ttgggcatgg ggggcgcctt cgcggccgtc gccgccgacg
     4261 cctccgcggt gttctggaac cccgccggta ttgccggcat ggagaaacgg caggttctcg
     4321 cgatgcacgc cgagcaattc ggcgacctgt tgaattacaa cttcgcgtcg tacgtccagc
     4381 cgaccgggct ggtcgacgag gcgcgcaagc ccgccttcgg cttcgggctc attcacctgg
     4441 gcgaccccga ccaactgctc acgagtcagt atcgctccgt cgacaacgac agcgacggcg
     4501 aaatcgatta cctgatcgac gaggacggca acgtcgtgga ctacgacgcc atcccgaggg
     4561 agtccaacaa cagcttcgcg gggttgggga cgttcgcgct cgagacgagt gcgggctcgg
     4621 tgggtgggac gctcaagctg atctaccagg atttcatcgc gggcgaatcg agcatgggaa
     4681 tcggcatcga cctgggcttc ttgcgccgcg atttcttgac cgaaaatctc tccgtaggag
     4741 tgaagctcgc ggacgccacc ggcacctaca tcagctggag cacgggcacc aacgagttca
     4801 tcgtgccctc actcaagctg ggcagcgcgt accgcatcga gtcggcctcg ctcaacgggt
     4861 cggtgctgtt cgcggttgac gccgacctct ttttcgacga ccgccagacc gcgtcgcaat
     4921 tctggagcga gacggtcagc accgacctgc ggctgggggt ggagttgagc ttccaggaga
     4981 aagtgatgct gcgcgggggg ctcgacgcgg agaaccccac cgcgggagcc gggctctact
     5041 tcggggtgtt cggcctcgac tacgcgtatc tgcaccacga cgcgttcgac tcgacgcacc
     5101 gcgtgtcgtt gctcgccagc ttctagggga ttcggaactc tagcgcgcac cgttttcgga
     5161 cggggtggta ccgcgcttgt agacgacgag cctctcgccc gccttgatca tcgagctccc
     5221 gcggttgttc cacgccatca ggtcctgcac gcccacgttg tactggcggc tgatcgagaa
     5281 cagcgactcg ccgcgcacca ccacgtgcgt gagcttggtg acgtcgtcgc cgggcgtcgg
     5341 cgcggacgcc ggcgccgggg atgcgacctc ggtcgtcgcg gctcgattcg tgtagatcgt
     5401 gagacgctgg cccgcgtaga tgtggcgccg gcggctcaaa ttgttccacg agcggatgcg
     5461 cgacacgctg gtgccgaagc gctgcgcgat ttcactgagc gtatcgccgc tcttcacccg
     5521 atac
//
LOCUS       DASPGK010000165         5611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_278715, whole genome shotgun sequence.
ACCESSION   DASPGK010000165 DASPGK010000000
VERSION     DASPGK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278715"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VEC56_04740"
     CDS             complement(<1..1274)
                     /locus_tag="VEC56_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXV13491.1"
                     /translation="MTRLLIAFAAVAMLAGIARAVEISPAFPNLAAFDSPINIEDPLD
                     GTDRLFVVERAGQIYVFQNDPGVSQRSLFLDISSLLTTQGECGLLGLAFHPDYETNRF
                     FYVCYVDASPFQTVIARFTANAANPNFADLGSQVPALTISQNGFFHKGGCIEFGPDGY
                     LYISLGEDGTPNNAQSLTTLKGKVLRIDVDSPGGGLQYGIPPDNPFEGNPNGYREEIY
                     AFGFRNPWRFSCDSQTGQLWLGDVGQNTWEEVDVILKGRNYGWPRMEGRVCYPIGQPC
                     DTTNLDLVLPVAVYNHGGNGASITGGCIYRGPSVPSLYGEYVFADYIDGRIWHLDPEV
                     NPSSIQLIDDTALNIAAFGTDASGELYFTTFNGAIYRFVESTTDVPAPAPAMGALHAV
                     HPNPFQSSAIMEYSIASAGRTMLEVFDVRGQIG"
     gene            1563..2027
                     /locus_tag="VEC56_04745"
     CDS             1563..2027
                     /locus_tag="VEC56_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13492.1"
                     /translation="MTDAIVAAILGGLIAIAGGFVAAWFQLRIARRTRMHAIIAEKKV
                     KANAEAYQYMKEIERSLIQRSDEETLLLLNRHDAWLAANRLFLPGSFPEKWTSLRITL
                     QWLVGSPMEGPRDPDTAHRLRLKALRLAKEALAEVYEDMEIEDRGGTTINDE"
     gene            complement(2130..2975)
                     /locus_tag="VEC56_04750"
     CDS             complement(2130..2975)
                     /locus_tag="VEC56_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13493.1"
                     /translation="MRRKLALAVLGGLVMGACSASKRVDTEPAAVHIVATDSGYVAPD
                     TLHEGLNHLVFENRGTTIHECMFVRLPDGMSARAYLDAVEAGYAFPEGAIDCSGPGLT
                     SPMQRFELWVPLEAGTYLLACWFTGHLTTTEPRTVVVHGAPRVAVTPPREDVTLKLID
                     FRFELIGEIETGPQTIRVEAAGPSMHEVDIFHLQEGRSVDVVRAWYASHQRGSLPATL
                     MGGVLDSHEIPSEVWLRREFAAGRHVLWCGMPMIQSGKESDAGAAADVSHADAGMVLE
                     FDVGK"
     gene            complement(2981..4165)
                     /gene="kbl"
                     /locus_tag="VEC56_04755"
     CDS             complement(2981..4165)
                     /gene="kbl"
                     /locus_tag="VEC56_04755"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HXV13494.1"
                     /translation="MNTIEPILNAELAAIREAGLYKDERVIQSAQAAAIKVAGGEVIN
                     FCANNYLGLSSHPQVIEAAHKALDQWGYGLSSVRFICGTQEIHKQLERGIASFLRTDD
                     AILYGSCFDANGGLFETLLGDKDVIISDELNHASIIDGVRLCKAERMRYGNGDMNHLE
                     DRLKEAKNFRVRMIATDGVFSMDGHIAKLKDICDLAEKYKAVVMVDDSHATGFMGKTG
                     RGTPEHHGVMERVDIVTSTLGKAMGGASGGFTTGRKEIVEMLRQRSRPYLFSNSLAPP
                     IVAASIAVLGLLEKSTELRDRLEENTRYFRTGMTAAGFDIIPGTHPICPIMLGDAKLA
                     QDVARDMLAEGIYVIGFSYPVVPKGKARIRVQISAAHTKQHLDRALEAFRKVGNKHGL
                     VK"
     gene            4281..5168
                     /locus_tag="VEC56_04760"
     CDS             4281..5168
                     /locus_tag="VEC56_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXV13495.1"
                     /translation="MERIRVATLQYYVRPISSFDQFRDQVAGLVETAEDYKAHLLVFP
                     EYFTVQLLTLQDIRRPIREQIRTLARQRDRFVEVMSGLARKYKIHIAAGTIPSDGENG
                     ALYNECFFFGPSGDHASQFKIHMTRFEKEEWLVQPRHEFRLIETVFGKVAINICYDVE
                     FPELARVAAREGASILIVPACTDDRQGFLRVRYCAQARAIENQMYVIHSATVGSLPMV
                     PAVSLNYGQASILTPSDFPFSRDGILAEGIANQETMVIGELNLTTLTESRETGTVLPL
                     RDSQASADIKKIVEHVKVS"
     gene            5179..>5611
                     /locus_tag="VEC56_04765"
     CDS             5179..>5611
                     /locus_tag="VEC56_04765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV13496.1"
                     /translation="MSFTVRELTPADFDRVAALGRKIYPHGTPWAPEQLSSHLRIFPE
                     GQFVVIENETGDLVGYAATLVVFWDDYDVDADWRDFTNHGMFTNHDPGRGRTLYAADI
                     MIDPDHQGKGAGKRLYAARQELLRRMGLLRIRAGARLQGYHR"
BASE COUNT         1007 a         1786 c         1779 g         1039 t
ORIGIN      
        1 ccgatctggc cccgcacgtc gaagacttcc agcatcgtgc gccccgcgct tgcgatcgag
       61 tattccatga tggcgctcga ctggaacggg ttggggtgga ccgcgtgcag cgcgcccatg
      121 gccggcgccg gcgcgggtac gtcggtggtc gattcgacga atcgatagat cgcgccgttg
      181 aaggtggtaa agtacaactc cccgctcgcg tccgtaccga acgcggcgat gttgagcgcg
      241 gtgtcgtcga ttagctggat ggaactcggg ttcacttcgg gatcgaggtg ccagatccgt
      301 ccgtcgatgt agtcggcgaa gacgtactcg ccgtacaggc tcggcacgga cgggccccga
      361 tagatgcagc cgcccgtgat cgacgcgcca tttcctccgt ggttgtacac cgcgaccggc
      421 agcacgagat cgaggttggt cgtgtcgcac ggctggccga tcgggtaaca cactctgcct
      481 tccatccgcg gccagccgta attgcgtccc ttgagaatga cgtcgacctc ttcccacgtg
      541 ttctggccga cgtcgcccag ccacagctgg cccgtttgcg aatcgcagct gaagcgccac
      601 ggatttcgga atccgaacgc gtagatctcc tctcggtagc cgttcgggtt gccttcgaac
      661 gggttgtcgg gcgggatccc gtactgcagg cccccgcccg ggctgtccac gtcgatgcga
      721 agcaccttgc ccttgagagt cgtcagcgat tgcgcgttgt tcggggtgcc gtcttcgccc
      781 agcgaaatgt agagatagcc gtcggggccg aactcgatgc aaccgccctt gtgaaagaag
      841 ccgttctgcg aaatcgtcaa cgccggcact tggctgccga ggtccgcgaa gttcgggttg
      901 gccgcgttcg cggtgaaacg tgcgatcacg gtctggaacg gcgatgcgtc cacgtagcac
      961 acgtagaaga accgattggt ctcgtagtcg gggtggaacg cgaggcccag gagtccgcac
     1021 tcgccttggg tcgtgagcag cgaggagatg tcgaggaaca acgatcgctg cgacactccc
     1081 gggtcgtttt ggaacacgta gatctgcccc gcgcgctcga ccacgaaaag ccggtccgtg
     1141 ccgtccaggg gatcctcgat attgatgggc gagtcgaagg ccgcgagatt gggaaacgcc
     1201 ggcgagatct ccaccgcgcg cgcgatcccg gccagcatcg cgaccgcggc gaaggcaatg
     1261 agtagtcgtg tcatggtgct ctcgcgttgg gaaatacgcg agcctccttg cacatcaata
     1321 tagacaaccc ggtcgccgtg cgggtgtgag gtcgttcacc ggtagccaat aattcacgcg
     1381 cggttcataa ggagcgagga agttcacgcc atcgacggca accattggag cgagcgtcgg
     1441 cggccgcgga ccttgcgcat cccggattcg accgcgacta ttcctatgtg agcaaagtca
     1501 ctcgttgcag ggtcggcggc ggtcgcgaag gccgtcggcc caattgggga gcgatcggtt
     1561 gaatgacgga cgccatagtg gccgcgattc tgggcggcct catcgcaatc gcgggtggat
     1621 tcgtcgcggc gtggtttcag ctgcgcatcg cgcgacgcac acgcatgcac gcgatcatcg
     1681 ccgagaagaa ggtcaaggcc aacgcggaag cgtatcagta tatgaaagag atcgagcggt
     1741 cgttgatcca gcgatccgac gaggagacat tgctgctgtt gaaccggcac gacgcatggc
     1801 tggcggcgaa ccgtctcttc ctgcccggat cgttcccgga gaaatggacg tcgcttcgaa
     1861 tcacgctgca gtggctggtc ggtagtccga tggaggggcc gcgcgacccg gacaccgcgc
     1921 accgcctgcg gctgaaggcg ctgcgcctcg cgaaggaagc gctggcggaa gtctacgagg
     1981 acatggagat cgaagaccgt gggggaacaa caatcaacga cgaatgagtc gggcttcctc
     2041 cgcgcggcgg gatcccaccg caaatcgaga ccgccggcgt ccgccgatcc gcgacatgcg
     2101 cgttcggcgc ttctttcacc gtcgtcttcc tacttgccca cgtcgaactc gagcaccatc
     2161 cccgcgtcgg cgtgcgacac gtcggccgcg gcgccggcgt ccgactcctt accgctctgg
     2221 atcatgggca tgccgcacca gagcacatga cgtccggctg cgaactcgcg acgcagccag
     2281 acctcactcg gaatctcgtg gctgtccagc acgccgccca tcagggtcgc ggggagggaa
     2341 cctcgctggt gactcgcata ccacgcacga acgacgtcga cacttcggcc ctcctggagg
     2401 tggaagatat cgacttcgtg catcgacggc cccgccgctt cgacccgaat cgtttgtggg
     2461 ccggtctcga tctcgccgat caactcgaag cggaagtcga tgagcttgag cgtgacatcc
     2521 tcgcgcggcg gcgtcacggc cacgcgcgga gcgccgtgaa cgacgactgt gcgtggctcg
     2581 gtcgtcgtga gatggccggt gaaccagcac gcgagcaggt aggttcccgc ctcgagcggg
     2641 acccatagct cgaatcgctg catcggcgac gtgagcccgg gccccgaaca gtcgatggcg
     2701 ccttcgggaa aagcgtagcc cgcctcgacg gcgtccaggt atgcgcgtgc gctcatgccg
     2761 tcagggagac ggacgaacat gcattcgtgg atcgtcgtgc cgcgattctc gaaaacgagg
     2821 tggttgaggc cttcgtggag cgtgtccggt gcgacgtagc cgctgtcggt cgcgacaatg
     2881 tgaactgcgg ccggctccgt gtccacgcgt ttcgatgcgg aacatgcgcc catcaccaac
     2941 cctcccaaga ccgcgagcgc gagcttccgg cgcatcgttc ctacttcacc agcccgtgct
     3001 tgttcccgac tttccggaac gcctccagcg cgcgatccag gtgctgcttg gtgtgcgccg
     3061 cggaaatttg tacgcggatg cgcgccttgc ctttcggtac caccgggtag ctgaagccga
     3121 tcacgtagat cccctcggcg agcatgtcgc gcgcgacgtc ttgcgcgagc ttcgcgtcgc
     3181 ccagcatgat gggacaaatc gggtgcgtgc ccgggatgat gtcgaagccg gccgccgtca
     3241 tgccggtacg aaaatagcgc gtgttctctt ccaggcgatc gcgcaactcg gtcgatttct
     3301 cgagcaagcc cagcaccgcg atcgacgccg ccacgatggg aggcgccagc gagttcgaaa
     3361 acagatacgg acgcgagcgc tggcgaagca tctcgacgat ttccttgcgc ccggtcgtga
     3421 agccgcccga agcgccgccc atcgccttcc cgagcgtcga ggtcacgata tcgacgcgct
     3481 ccatgacgcc gtggtgctcc ggcgtcccgc gccccgtctt gcccatgaac ccggtcgcgt
     3541 gcgagtcgtc caccatgacg accgctttgt acttctccgc caggtcgcag atgtccttga
     3601 gcttcgcgat gtggccgtcc atgctgaaca cgccgtcggt tgcaatcatg cgcacgcgga
     3661 agttcttcgc ctctttcaga cgatcctcga ggtgattcat gtcgccgttg ccgtagcgca
     3721 tccgctccgc cttgcacaat cgaacgccgt cgatgatgga ggcgtggttg agctcgtcgg
     3781 agatgatgac gtctttgtcg ccgaggagtg tctcgaagag cccgccattg gcgtcgaagc
     3841 aggagccgta gaggatggcg tcgtctgtcc tcaagaacga cgcgattccg cgctcgagtt
     3901 gtttgtgtat ttcctgcgtc ccgcagatga agcgcaccga cgagaggccg tagccccact
     3961 ggtcgagggc tttgtgcgcg gcttcgatca cctgggggtg cgacgacagt ccgaggtagt
     4021 tgttcgcgca aaagttgatc acttcaccgc cggcgacttt gatggcggcg gcttgcgcgc
     4081 tctgaatgac gcgctcgtct ttgtagaggc cggcctcgcg gatggcagcg agttccgcgt
     4141 tgagaatggg ttcgatggtg ttcatgggcg aattctagca caagcgcgcg aggtacgaaa
     4201 cggcgtgcca tggtcggggc gattcgactt gccccgcgcg ccgatctgtg tcaatgattg
     4261 gctgtcgacg gaggtcgatc gtggaacgaa tcagagttgc tacactccaa tactacgtcc
     4321 gtcccatctc gtcgttcgac cagttccgag accaggtggc cggcctggtc gaaaccgccg
     4381 aagactacaa ggcgcacctc ttggtcttcc ccgagtactt caccgtccag cttttgaccc
     4441 tccaagacat ccggcgtccc atccgcgagc agatcaggac cctcgcgcgc cagcgcgacc
     4501 gcttcgtcga agtgatgtcg gggctggcca ggaagtacaa gatccatatc gccgccggta
     4561 ccatcccgtc cgacggggaa aacggcgcgc tgtacaacga gtgcttcttc ttcggcccca
     4621 gcggtgatca cgcatcgcag ttcaagatcc acatgacgcg cttcgagaag gaagagtggc
     4681 tggtgcagcc gcgccacgag tttcgtctca tcgagaccgt gttcggcaag gtggccatca
     4741 acatttgcta cgacgtcgaa tttcccgagc tggcgcgcgt ggccgcccgc gagggcgcga
     4801 gcattctgat cgtgcccgcg tgtaccgacg atcgccaggg tttcctgcgc gtccgctact
     4861 gcgcccaggc gcgcgccatc gagaaccaga tgtacgtcat tcactccgcc accgtcggct
     4921 cgctgcccat ggtgcccgcg gtgtcgctca actatggcca ggcgtcgatt ttaacgccca
     4981 gcgacttccc cttctcccgc gacggcatcc tcgccgaagg catcgccaac caggaaacca
     5041 tggtgatcgg cgaattgaac ctgaccacgc tcacggaatc gcgcgagacg ggtaccgtgc
     5101 tgccgttgcg cgacagccag gcttccgccg atatcaagaa gattgtcgaa cacgtgaagg
     5161 tgtcgtgagg gagggcgaat gtcgttcacc gttcgcgagc tcacgccggc cgatttcgac
     5221 cgcgtcgccg cgctcggccg caagatctac ccgcatggaa caccgtgggc gcccgagcag
     5281 ctctcatcgc acctgcgcat ctttcccgag gggcagttcg tcgtcatcga gaacgaaacc
     5341 ggagacctag tcggctacgc cgccaccctc gtcgtatttt gggacgatta cgacgtggac
     5401 gccgactggc gcgacttcac caaccacggc atgttcacca accacgaccc cgggcgcggg
     5461 cgcacccttt acgcggccga catcatgatc gaccccgacc atcaggggaa aggggccggc
     5521 aagcggctct atgcggcgcg tcaggagctt ctgcggcgca tgggactgct ccgcattcgc
     5581 gccggcgctc gcctgcaggg ctaccaccgc t
//
LOCUS       DASPGK010000166         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1565449, whole genome shotgun sequence.
ACCESSION   DASPGK010000166 DASPGK010000000
VERSION     DASPGK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565449"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VEC56_04770"
     CDS             <1..403
                     /locus_tag="VEC56_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13497.1"
                     /translation="KEALFRYTVVAQVRAQMLAGVCREDAIAEVAERSHARVDGGEPE
                     EVTQRRLQRWTSAYEAAGIAVLEPKSRRRTNTSLALDEKLIDFLRRERATDSDASIPE
                     LLRRAKALGVIEGCGSFATSRIRSDLLDIAS"
     gene            479..2065
                     /locus_tag="VEC56_04775"
     CDS             479..2065
                     /locus_tag="VEC56_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HXV13498.1"
                     /translation="MQPQAINEEKPFAEADAKFNELKTRLGSESSRLLSHSEIEELLQ
                     VEGREVLRLLYQGHLDLRGPGDVDGDVVGGDRVVRVAGRIHDRKLQTVFGTVEVSRRS
                     YAAEGHASLHPLDADLNLPSERYSHGTRRRVAEEIRKQSFDETVDELARTLGEPVPKR
                     QVEELTIRAAQDFDGFYEKRKAASTREVAKTGDIVVISSDGKGVPMHTGDLRPATKKA
                     AEKQAADNANEKPFSLGRPRSSEEARDRKRMATVAAVYTTAAFFRTPEDVVRELNHLQ
                     VVDSGESAKRPKPERKRVWASLEKTPHEVLGAAFTEALRRDPKRKKLWAALVDGNETQ
                     LDAIHALEREHELTISIIVDFIHVAGYVWKAAHAFHPRGSKDAETWVRDHLLEILRGR
                     SSAVAGGMRRSATLRGLSGKERESVDDCADYLIKYRAFLHYDEYLAAGLPIATGVIEG
                     ACRYLVKDRMEITGAVWGLASAEAVLKLRALHASGDFDEYWSFHKDKEFRRNHASHYA
                     NSRPPAVRNTTRHGRLRLAT"
BASE COUNT          459 a          675 c          763 g          344 t
ORIGIN      
        1 gaaggaggcc ctgttccgct acaccgtcgt cgcgcaggtc cgcgcgcaga tgctggccgg
       61 cgtttgccgc gaggacgcga tcgcagaggt cgccgagcgc tcgcatgcgc gggtcgacgg
      121 cggcgagccg gaagaggtca cacagcggag gctccaacgg tggacgtccg cgtacgaagc
      181 cgcgggaatc gcggtgctcg agcccaaatc acgccgtcgg acgaacacct cgctcgcgct
      241 cgacgaaaag ctgatcgact tcctccggcg cgagcgcgcg acagattctg acgcctccat
      301 ccccgagctg ctccggcgcg ccaaagcgct gggcgttatc gaggggtgcg gatcttttgc
      361 gacgagccgg attcgatctg atctcctgga catagccagt tgatcgatgg tgaggagggg
      421 ccgaaccgtg gcacgtacgg gttgcgacac tcgatacgac caaggaggcc ccgacgccat
      481 gcaaccacag gcgatcaacg aagagaagcc cttcgcggaa gcggacgcca agttcaacga
      541 gctcaagacg cggctgggat cggagtcgag ccgtctgctc agccacagcg agatcgagga
      601 acttctgcag gtcgagggac gggaggtgct ccggttgctg taccagggac atctggatct
      661 gcggggcccc ggcgatgtcg acggcgacgt tgtgggcggc gatcgtgttg tgcgggtggc
      721 gggccggatc cacgaccgga agttgcagac ggttttcggg acagtggagg tgagccgaag
      781 atcctacgca gccgaggggc acgcgagcct gcatccgctg gacgccgacc tgaacctgcc
      841 gagcgagagg tattcgcacg gtacgcgccg acgggtcgcc gaagagatcc gcaaacagtc
      901 gtttgacgag acggtcgatg aactcgcgcg gacgctcggt gagccggtgc cgaagcgcca
      961 ggtcgaggag ctcacgattc gcgccgcgca agatttcgac gggttctacg aaaaacgcaa
     1021 agcagcgtcg acacgtgagg tggcgaagac cggcgacatc gtcgtgatca gcagtgacgg
     1081 caagggtgtg ccgatgcaca ccggggatct gcgtcccgcg acgaagaagg ccgccgagaa
     1141 gcaggccgcc gacaacgcga acgaaaagcc gttctcgctg gggcgaccga ggtcgtcgga
     1201 ggaagcccgc gatcgaaaac ggatggcgac ggtggctgcc gtgtacacga cggcggcgtt
     1261 cttccgtact cccgaggacg tcgttcgcga gttgaatcat ttgcaggtcg tcgattccgg
     1321 cgaatcggcg aagagaccca agccggaacg gaagcgtgtc tgggcaagtc tcgagaagac
     1381 gcctcacgaa gtcctcggcg ccgccttcac ggaggcactg cgacgcgatc ccaagcgaaa
     1441 gaagctgtgg gccgcgctcg tcgacggcaa tgagacgcag ctcgacgcga tccacgcact
     1501 cgagcgcgaa cacgagctca cgatcagcat catcgtcgac tttattcacg tcgctgggta
     1561 cgtatggaaa gcggcgcacg cgttccaccc ccgtgggagt aaggacgccg agacatgggt
     1621 gcgcgaccac ctcctcgaga tccttcgcgg tcgttcgagc gcggtggcgg gtggtatgcg
     1681 acgaagcgcg acgcttcgcg gactctcggg gaaggaacgc gagtccgtcg atgactgtgc
     1741 ggattacctg atcaaatacc gcgcgttcct ccactacgac gagtacctcg cggccggtct
     1801 tcccatcgcg acgggcgtga ttgagggcgc gtgccgatat ctcgtgaagg accgcatgga
     1861 gatcaccggc gcggtgtggg gcctcgccag cgccgaggct gtcctgaagc tccgcgcgtt
     1921 gcatgccagc ggggacttcg acgagtactg gagcttccat aaggacaagg agtttcgacg
     1981 gaaccacgcg tcccattacg cgaactcacg accgcccgcc gtcaggaaca ccacgcgcca
     2041 tggccgcttg agactggcca catgatcagg gctcatgaat gtcgaagaag agccgcaccc
     2101 catcaaaagg cttggggctg gagaccatct ggtggaggtc gaaatttctc cggcgtccag
     2161 aaaggaagac ccgagtctcc caaagacgat gcagatgcgc gcgatccgct accatcgcag
     2221 gggcttcaag ccgcaggtcc t
//
LOCUS       DASPGK010000167         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1448257, whole genome shotgun sequence.
ACCESSION   DASPGK010000167 DASPGK010000000
VERSION     DASPGK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448257"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VEC56_04780"
     CDS             complement(<1..832)
                     /locus_tag="VEC56_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXV13499.1"
                     /translation="MTRFSVFQGLAVALAALSIAATSARSQLGFTEFALTDTTLNSDP
                     STDFWIASAAPADVDADGDLDLLVAGYYVIYFVSAEDRLVLYRNDGPVDASTWELTPI
                     AVDATGLSFTAADIAWGDYDNDGDPDAVVAGNGATTLYRNDAGTLLRTPTVLPEYWED
                     SDFSTMDLRSLTWADYDNDGDLDLLVPSVVGEFVYAPTAVLRNDGAGTGDAWVFTDVG
                     ASLPATSNAVSVWADMEGDGDLDLLLANVSPWGDNFMNTYRNDGASFTEIDTALAFIR
                     Y"
     gene            complement(1186..>3001)
                     /locus_tag="VEC56_04785"
     CDS             complement(1186..>3001)
                     /locus_tag="VEC56_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HXV13500.1"
                     /translation="DANTQRAIAGATVLVEGTRLTTHTGNDGGFLIANVPAGTYTVRV
                     DALGLTSAKQRVNVVAGRTATADFHMLSLRTRAEAPVEAETSADERAGRVPASREMKK
                     DALGYPSLGNNAPLQCYTPPMAWPSPGFEDYDVIHENGWTWVRTRPLSTFSVDVDAAS
                     YGNVRRFITQGQLPTIDAVRIEELVNYFDYDYPNPRGRHPFSITTEVAQCPWNPGNKL
                     VHIGLQGARVDVEDLPPSNLVFLIDVSGSMNQPNKLPLLKSSFRMLVDQLRHEDRVAI
                     VVYAGAAGLVLPSTRGSDKETILCAIDRLEAGGSTAGGAGIRLAYHVAQENFLGDGNN
                     RIILATDGDFNVGAASDGDMIRLIEEKRGSGVFLTVLGFGEGNLKDSKMEKIADHGNG
                     HYAYIDDVAEARKVLVNEMGASLLTIAKDVKLQVEFNPARVSSYRLIGYENRVLADRD
                     FDDDAKDAGEIGAGHSVTALYEVTLADREDDRAGKKFKYSDVTVKDNARRSAELLTVS
                     FRYKLPTESESSLLSVTVKDRNIRFANASNDFRFSAAVAQFGMILRDSPERGDATMRD
                     VIATARAARGEDAHGYRAEFVALAEMAEALMTPTVGVR"
BASE COUNT          520 a          968 c          968 g          545 t
ORIGIN      
        1 cataccggat gaaggcgagg gccgtatcga tctccgtgaa cgatgctccg tcgttgcggt
       61 acgtgttcat gaagttgtcg ccccacggac tgacattcgc gagcaggaga tcgagatcgc
      121 cgtctccttc catgtcggcc catacgctca cggcgttgga cgtcgcggga agggacgcgc
      181 ccacgtcggt gaacacccac gcgtctccgg tgccggcgcc atcgttccga agaaccgccg
      241 tcggcgcata gacgaattcg cccaccaccg aaggcactag aagatcgaga tcgccgtcgt
      301 tgtcgtagtc ggcccacgtg agcgagcgca ggtccatggt ggagaaatcg ctgtcctccc
      361 agtactccgg aaggaccgtg ggcgtgcgca gcagcgtgcc cgcgtcgttg cgatagagag
      421 tcgtcgcgcc gttaccggcg acgaccgcgt cggggtcgcc gtcgttgtca tagtctcccc
      481 acgcaatgtc cgcggccgtg aagctgaggc cggtcgcgtc caccgcgatc ggggtgagtt
      541 cccacgtcga ggcgtctacg ggtccgtcgt tccgatagag cacgagtcgg tcttcggcgg
      601 agacgaaata gatcacatag tagcccgcga ccagcaagtc gagatccccg tcggcgtcga
      661 cgtcggcggg agccgccgag gcgatccaga agtcggtcga cgggtcggaa tttagagtgg
      721 tatcggtcaa cgcgaattcc gtgaagccga gttgggatcg cgccgaagtt gcagcgatac
      781 tgagtgcggc cagtgcaacc gccagcccct gaaagaccga gaatcgggtc atggttccac
      841 ccccggggcg caagaagtag ttgaagatcg tttactcctg atagtgtagt ccagggtcaa
      901 ccagcagaca agccccacct tgtcaaattg ttgcagctct ttcacaaggt attacgcacc
      961 attgcgttac gccatggtat ccgcctcgcg ccgcccgacc gcgccgctgg acaatcggga
     1021 tcgagactcc gggtcgggat gggtcggccg acggtcaaat tggaattgtc gaaggcggca
     1081 tcgccaccag cagtgacgcg cgacacggca aagcaacagg ccgcccctct tgcgaggagc
     1141 ggcctggtcc gcggaaagcc cgcgcccccg cgcttcccgc gtgaactacc ggacacccac
     1201 cgtcggcgtc atgagagcct ccgccatctc ggcgagggcc acgaactccg cgcgataccc
     1261 gtgtgcgtcc tcgccgcgcg ccgcgcgcgc cgtggcgatg acgtcgcgca tggtcgcgtc
     1321 gccgcgctcg ggcgaatcgc gcaggatcat cccgaattgc gcgaccgcgg ccgagaaacg
     1381 gaagtcgttg gaagcgttcg cgaagcggat gttccgatcc ttgaccgtca cggagagtag
     1441 agagctctcg gattcggtcg gcagcttgta ccgaaacgag acggtgagca gctccgcgct
     1501 tcgacgcgcg ttgtctttaa cggtgacgtc cgagtacttg aacttctttc cggcgcgatc
     1561 gtcttcgcgg tccgccagcg tgacctcgta caaagccgtc accgagtgcc cggccccgat
     1621 ctcgcccgcg tccttggcgt cgtcgtcgaa atcgcgatcc gcgagcacgc gattctcgta
     1681 gccgatcaat cggtacgacg acacgcgcgc ggggttgaac tcgacctgca gcttgacgtc
     1741 cttcgcgatg gtcaggaggg aggcgcccat ctcgttgacg agcaccttgc gcgcctcggc
     1801 gacgtcgtcg atgtacgcgt agtggccgtt gccgtggtcg gcgatcttct ccatcttcga
     1861 gtccttgagg ttgccctcgc cgaacccgag cacggtcagg aagacgccgc tcccgcgctt
     1921 ctcttcgatc agacgaatca tgtcgccgtc gctcgcggcg cccacgttga agtcgccgtc
     1981 ggtggcgagg atgatgcggt tgttgccgtc cccgaggaag ttctcttggg cgacgtggta
     2041 cgcgagccgg atgccggcgc cgcccgcagt cgagccgccc gcttccaagc gatcgatggc
     2101 acacagaatc gtctctttgt cactgccgcg cgtcgacggc agcacgagcc cggccgcacc
     2161 cgcatacacc acgatcgcga cgcgatcctc gtgacgcaac tggtcgacga gcatgcggaa
     2221 cgacgatttc aggagcggca gtttgttggg ctggttcatc gagcccgaca cgtcgatcag
     2281 gaacacgagg ttgctgggcg gcagatcctc gacgtcgacg cgtgcgcctt gcagaccgat
     2341 gtggaccaac ttgttaccgg gattccacgg gcactgcgcc acttcggtcg tgatcgagaa
     2401 gggatgacgt cctcgcggat tgggatagtc gtagtcgaag taattgacga gctcttcgat
     2461 acgcaccgcg tcgatggtag gaagttgtcc ctgggtgata aagcgacgga cgttgccgta
     2521 cgacgccgcg tcgacgtcga cggagaaggt cgaaagcggc cgggttctca cccacgtcca
     2581 cccgttctcg tggatcacgt cgtagtcctc gaagcccggc gacggccagg ccatcggcgg
     2641 cgtgtagcac tggagcgggg cgttgtttcc cagggacgga taacccagcg cgtccttttt
     2701 catctcgcgc gacgcgggca cgcgccccgc gcgctcgtcg gcggatgtct ccgcctcgac
     2761 cggtgcttcg gcgcgcgtgc gcagcgatag catgtggaag tcggccgtcg ccgtcctccc
     2821 ggcgaccacg ttcacgcgct gcttcgcgct cgtcaaaccc agcgcgtcta cccgcacggt
     2881 gtacgtcccc gcgggaacgt tcgcgatcag gaagccgccg tcgttcccgg tgtgggtcgt
     2941 gagacgtgtt ccctcgacga gcaccgtggc tcccgcgatc gcgcgctgcg tgttcgcatc
     3001 g
//
LOCUS       DASPGK010000168        18829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_395270, whole genome shotgun sequence.
ACCESSION   DASPGK010000168 DASPGK010000000
VERSION     DASPGK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 18829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18829
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395270"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VEC56_04790"
     CDS             <1..1679
                     /locus_tag="VEC56_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV13501.1"
                     /translation="DTRVDDLRGLGIYLVVEETEALYLLCARDAHARVRVGGVFVPLH
                     ETTQDRCSIRGEEMDIETTRAQHDLFAQTLPDALVLYRIPRPQDGTELALLLGGSPGD
                     LAAALDALDARPAGAASLESDRIARSLVCLRFDARRATRDDGDVRVARRSPGAWTRGA
                     VAALLVVGGAVGVGLVATRVDFERPPLAHNDADAERTARATQERPTPVMTRDASVSEA
                     TAEVAAVDERAREEADHPRFALAWEKSYSEPVTSSPSLLGDGVVFGSRDGRVYAVDRG
                     NGKQMWSHAAVGGVGASPLVRGDAVVVADYGGNVYRLARGDGRTLWKRALKERIVSTP
                     AATGERVAVGTSRGNVYALSFETGRVLWKFAARGQVRGGIAHAGGTFFVPSHDGRLYA
                     LADDTGRKRWSLALGGPVASTPFTDGTRVVVGTAQGSVVALAHADGKRLWSFATRGPV
                     NSAVLIHDGRVYAGSADEHVYCLDAGTGKLIWSFETSGTVLSRPFVDGKRVVVTSYDG
                     SVYALDASDGTLVDRYPTEQSIFSSPVVDGDRVYFGNNAGRFFCLNLQDS"
     gene            1787..4897
                     /gene="secA"
                     /locus_tag="VEC56_04795"
     CDS             1787..4897
                     /gene="secA"
                     /locus_tag="VEC56_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXV13502.1"
                     /translation="MSLLQKLFKPKSQRDFKRHGDLLEQVKARAEEYRGFTPEQFRAK
                     SDEFRLRLQEGATLDDLLPEAFALVKSACQALVGTTWSVVGIPTKWEMVPYDVQVVGG
                     IVLHQGKIAEMATGEGKTLVATMPLYLNALAGRGVHLVTVNDYLARRDSEWMGKLFEL
                     LGISVGVIQNDMPNDLRKQMYARDITYGTNNEFGFDYLRDNMKTRADDRVQRGHPYAI
                     VDEVDSVLIDEARTPLIISGPVSHSQSSDLFARLKPKVERVVALQNRLVNDLVAFAEK
                     NLENEEQADEVARALLKVKRGSPKSNRFMKLMAEPGTERMIQRIEVELMRDKVLHELD
                     EELYFAVDEKGNSINLTEKGREALSPEDREQFVLPDLSEAIAAIDADESLSPADRVRA
                     KDDAHRAYAERSEAIHNFSQLLRAYTLFQKDNEYVVQEGRVLIVDEFTGRLMPGRRFS
                     DGLHQALEAKEGVKIEGETQTLATITLQNYFRMYQKLAGMTGTAETEAEELHKIYKLD
                     VQVVPTNEMVRRVDYDDLIFKTKREKYNAIVDEVARLHEQKLPVLVGTISVEVSETLS
                     RMLKRKGIMHNVLNAKFHQQEAEIVAKAGRAGAVTIATNMAGRGTDIKLEGSVVRCTE
                     CGIGSGRKDWQTKDGGTVDTVACAQDVPCGLHIIGTERHESRRIDRQLRGRAGRQGDP
                     GASVFYISLEDDLMRLFRPERIAGVMDRLGVKEGEVITHPLVTKSIARSQKRVENHNF
                     EIRKRLIDYDDVMNKQREVIYGRRNEVMDAQELKPIIETIIDDYVDTTIEKTVDSSQV
                     PGAAERTELLGQLENVFMYTFAHDSDHAETVEELREHAKAQAREALRAREELLARDLN
                     NPDLVREFEKFVLLQVIDEKWMDHLHELDSLKEGIHYRAYAQKDPLVEYKREAFGLFA
                     DLNERIDRDALYAMFHARVAAAPRPTADLARARAVHREAAPVLAPGGQGTSPLGTPSS
                     APAATPALNRPAQPPLGATEATSRVLRGAASTQPVVRTEDKVGRNDPCPCGSGKKYKK
                     CHGISS"
     gene            5038..5484
                     /locus_tag="VEC56_04800"
     CDS             5038..5484
                     /locus_tag="VEC56_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HXV13503.1"
                     /translation="MWTRLMDLVMLVAEMSRSADRRPVELDRELTRRGYSSEEIEHAV
                     FWISSRPEASRAMARPGRAMRVLSEFERMSLSTEAYDYLFRLQNLGIIDGRQFETILA
                     RAIPVGAEKVHVEDVKGIACSVIFNRDTGDVDEEAFDRFDTDGAPV"
     gene            5557..7788
                     /gene="topA"
                     /locus_tag="VEC56_04805"
     CDS             5557..7788
                     /gene="topA"
                     /locus_tag="VEC56_04805"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXV13504.1"
                     /translation="MANNALVIVESPAKANTIKKFLGKGFKVVASVGHVMDLPTNRLG
                     VDLDNGFSPEYVVSRGKKKILDQIKKDAKAADQVYLALDLDREGEAIAWHIREYIAPV
                     QRNVKRIIFNEITKGAIKSAIDDPMDVDMKKVDAQQARRILDRLVGYKISPILWQIFY
                     YGLSAGRVQTVGLRLVCEREAEIEAFVAEEYWTIEGVLTTPGGGELPVKLIEKNGEKI
                     ELKNQKQSDAALADLKKASYAVTSVEKKERKRNPQPPYITSTMQRDAARRLRFSAKKI
                     MMLAQQLYEGVDTGNGDRVGLITYMRTDSVRIADQAKESARDYIVKTWGEDYSPEKAR
                     AYKTSAKAQDAHEAIRPSDVERTPESLKANLTKDQYALYDLIWRRFLASQMESARYDT
                     TEVLVGAGAYTLKANGRVMTFPGFLAVYEESLGDEGGELPPVKEKDALAVKSIDGIQH
                     FTEPPPRYTEATLIKDLEDKGIGRPSTYANIVSIIQDREYVEKEEGRLRPTTLGRQVW
                     KTLERFFPEVFDTSFTAQMEQELDKVEGGEFTWQAVVKDFYQPFKESLEHLDDKKARL
                     KSELQEETDVKCEKCGRNLIKKWGRNGQFLACPAYPECKFSRPIADPSANVTVEGACP
                     KCGSQLVAKSGRYGRFVACARYPECKHTEAFPIGMKCPREGCGGKIVEKRTQRGKTFY
                     GCSNYRAPKEGQAPGCDWASWDKPVAEECSNCGSPYMVHKSSKTRGDFLKCPKCKEEM
                     ASE"
     gene            7822..9138
                     /gene="trmFO"
                     /locus_tag="VEC56_04810"
     CDS             7822..9138
                     /gene="trmFO"
                     /locus_tag="VEC56_04810"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXV13505.1"
                     /translation="MGAVTIVGGGLAGCEAALQLADRGFHVRLFEMRPETATGAHKTD
                     RLAEIVCSNSFKSTLLDTASGLLKAEMDALGCRLLPVARENSVPAGHALGIDRDLFSS
                     AVTARVEAHANITIERRRVDSLDLPVPAVVATGPLTAEALSQALVSHCSRDHLYFYDA
                     IAPSIDGSTVDPAVGYWASRYGKGDADYLNIPFSRDEYRRLIEFVRGAEYSQSHAFEE
                     EKYFEACLPIEVLVARGEDTLRFGPMKPKGLPDPRTGRDPYAVLQLRKESREGTLMGL
                     VGFQTRMTWACQKEMLHLLPGCSDVSVLRFGTIHRNIFLDIPTLCDPYLRDRARPGLH
                     FAGQICGVEGYVESIMSAIIVSLSIAAASRGREMPPIPSETMIGSLMDYVHTPRKNFQ
                     PMNANFGLLPAGAGGRRNRQTRHQDAAKAAIAAMESYRDRNAWLFE"
     gene            9254..10183
                     /locus_tag="VEC56_04815"
     CDS             9254..10183
                     /locus_tag="VEC56_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXV13506.1"
                     /translation="MLLDTAIERFRTHLAHERGLSPRTVEAYASDLASLSAFLAERGR
                     DRIQAGSVDATALRSYLAAEVARGLSGRSMMRRVSALRSFFRFLEKRGAIASDPTLHL
                     AQRVARRSVPTIVSEERIELMMGLPDVSSPAGARDRAILEFLYGTGVRLGELVALKVG
                     DFLPLSDRVVVHGKGSKRRVVPFAGSARESMLAYWASRFGLSRATDGSLEAYAGAPAL
                     AGRAKGDKESRVSRRTVQRVVGRHLRRVASVTQASPHVLRHAFATHMLDHGADLRAVQ
                     ELLGHESLSTTQVYTHVSIEHLRKVYRKAHPRA"
     gene            10176..11240
                     /locus_tag="VEC56_04820"
     CDS             10176..11240
                     /locus_tag="VEC56_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HXV13507.1"
                     /translation="MRSTRAALAVLLALACAVEEPPSGGPEDKAPPAVVETTPSADST
                     GVDPASPIAITFGEKMTRARIERLVTVQPPIEIERVRWEGNTLVIEPRGGLQRDTTYV
                     VRLKPGYRDSHGVTNTAGREFAFATGDAPLDTARISGTVLFKREPSGKAMVRVFRVPR
                     EADFRADAARPDREVATRRDGTYTLGHLPANDARFIVMAYIDQNGNAAFDKASEPFTV
                     LPDTLVLTGAVTEVAGVQIGVIDPNEPGLVRGTVANQTGIDSLLATVSLTATRDSTRV
                     QYLVRCDTSGAFEFARVKPGGYRLWAFLDLRADSARGHFDCGDTLTCPEPWATLPDSL
                     SVGPGATVDVGTLVLRRRDN"
     gene            11251..12087
                     /gene="dapF"
                     /locus_tag="VEC56_04825"
     CDS             11251..12087
                     /gene="dapF"
                     /locus_tag="VEC56_04825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXV13508.1"
                     /translation="MRIEFVKMNGAANDFILVDNRRDDVRLTGAQIARLCDRRRGAGA
                     DGFVSIEGGAAAQGRDFFMRFYNSDGTEAEMCGNGARCSARFAAELGLGKRHDHEVVL
                     DFMTGSGPIEARVRGARAWMRMMDAARMRRGIETRVKTPLGAVHFMTVGTRHAVVPVA
                     DAPAMTPAEVFELGRTLRYDPAFAPEGANVNFASIGKDGRIYLRTYEKGVEAETFACG
                     TGSVAASVLFAHEGRLNSPTRIVQHSGDELTASFELAPTGAVNVALEGPAEVNFHGWA
                     DV"
     gene            12099..14981
                     /locus_tag="VEC56_04830"
     CDS             12099..14981
                     /locus_tag="VEC56_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV13509.1"
                     /translation="MRTCLSTLALAAIAVCLAGSARAQYPFGKNKVIYQGKDWRVLET
                     PHVDIYHYAPDSTLILYLAPLVEETFLEFSETFRLEFRRRLPFVFYATHYDFQQTNIL
                     PVLISEYTGGFTDLMKGRIAVPFNGSYANLRHVVRHEMVHAFMLEKLQVHMHEMGKYT
                     FSHPPLWFVEGMAEYFANAPQNSQGEMFVRDALLNGRLYDLDEIWRIEGSFMMYKQGE
                     AVINYIATNFGSDAVIRILEAWWTAPEFTLVLTNAIGMDLYELNDAFMKYMKRKHYPA
                     VLTRQFASDQGLQLTPPRSFHSRPAGAFVDGPNADSTGAPIQEKVVYSLTGRDGVVVL
                     TRGHGSQRVTPATPGAAGVTAVADMPWNEHVVVAGGRSRELESLPAFRSKIEALGHTL
                     VFVSKSSARDRIYFWSARKGERIAHFEFDGLSIIQSPTVSGDRTKMVFSAIDTRGVMD
                     LYLVHLEDGRLERLTEDGFSEEDADYHPYDDVVLFASDRGAGQSKDRTHIYRMDLETR
                     EIAALEGGPFGDSNPEWAPDGNSFLFTSDRDGAANIYLREGDAVIQQTDVLTGVSAPS
                     FYPDGSGFVAVVYEGGEFQLYDFPIRRHTPVPASARPPDDLVVPWTRSDMPDSAFVTK
                     PYETRFSIDFIGAGVAIDPEAGDVGNGGAIVMTDVLGNHQLSFVFGTTTEEFNNFWDD
                     FNAAFAYLNLSHRVNYSLSFFHLNTYSDLRLLSQREKRVGGAFGISYPLDKFQRIESS
                     LVLRQIEISDLEALIRDSEKRSFTGSVFMSYVRDNAVWTFGGPLTGWRYYVTGGQTVD
                     FRDRGFDNVLLQVDARRYFKLGSRAAFALRYLNRSAWGGDEQVFYLGGPWTLRGYPYN
                     QFFGKTIQLVSAELRFPLLDGLRIVLPFGPIEFPMFRGSLFFDAGMTDRNEFKILDTE
                     WLGSVGTGIELNLGYAPVMRVNFTWPTDFDTISSDTGFELFIGYNY"
     gene            15018..16721
                     /locus_tag="VEC56_04835"
     CDS             15018..16721
                     /locus_tag="VEC56_04835"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXV13510.1"
                     /translation="MRWSKYYLPTYKEVPKDAEIPSHRLMLRAGMIRILTSGVYSFLP
                     MGYRVLRRVAAIVREEMDRIGSQEVQMPVLHPAELYEETGRLKNFGEILFQLKDRRAR
                     QFALGPTHEEVVTDIARAEIKSYRQLPQTLYQIQTKFRDEFRPRFGVMRAREFMMKDA
                     YSFHASDESLHDTYAAMAGAYRRILERCGLEGVQVQAESGAIGGDINEEFIVLADAGE
                     SVMFRCPSCGYAANDERAVSSGAATLEKEPSAEMEKVATPGQHTVDEVSAFFKVSKQR
                     LVKTLLYKSKWRDVAVLVPGDRELNEVKLVKALDDPEARPMTPEEVEALTGAPVGFAG
                     PVGLKAILIADDLLKDYDGMIVGANAADAHLRGVKMGRDFKPERTAPLSTVVAGDRCA
                     SCGKGELALQRGVEIGHIFKLDVKYSKAMHATFLDPEGQERPFVMGCYGFGVSRAVAA
                     AIEQHYDEKGIRWPKALTPFHVVVTPLNLNDEGTVKTMERVYADLQAAGFDVLLDDRD
                     LRPGPKFKDAELIGVPMRVTLGERNLKDGNCELYYRLEDKTDIVALADVTSKIRSYYA
                     N"
     gene            complement(16761..>18829)
                     /locus_tag="VEC56_04840"
     CDS             complement(16761..>18829)
                     /locus_tag="VEC56_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13511.1"
                     /translation="RMRCIPLILIAVLGTTVATSVAAGQEPRRTPTTALAPGWPLSEI
                     LGPRDRFQSNDDVTVLGLWTFEDGMGGPDAQGWTTYDRSSQLGTFFHVEDFNPAIPGM
                     GPLAGTKSLWCGARACPAVPEVCDYLTLPGYGNSWDQRFSSVALSVTGHVTLTFLLGF
                     DLEPSYDFIDLQYRSLTSTWQTLDSFTGLDYAPFEYSVLSSEHNGTVEFRFRVRSDAV
                     YSDEDGGYNSLGAAVIDDILVEDATGIVSYQQFEAEAEGALTTLDGEWFASPVPAKGD
                     YAELINGGSVLQEDPNVVNPSHVWSFFKNSPDDYTCGGHPGQAVVPFGSDGSFIRNEI
                     QSPVISLAPLAQPVTSLTLAFDVYRYLPLDNLVTYEYRVRSRVGGQWNDWRASGINYY
                     GPTAQWHVSAFEIAPLIDGGATDIQVAIGVRDLCGIWCGSVGSGACHTNAPLIDNVRV
                     ANFIANTPAGFDVVVTPEDVVTGTTPVTLTFNYIDQPGATTLTTGSLGPAVTGLFTLA
                     NGTYYDVSTSATFLGNVEICIAYDEGTLTVPEADLRLLHWDTELNPDAWVDITTSLEI
                     IGNTICGVTDHFSPFALVAPVPTAVGDAPVVLALHQNVPNPFNPNTTIAYDVPAGGAH
                     VSIEIYDVTGRAVRTLVDGPRPAGTHRVDWDGRNASGTPVASGVYLYRMTSGTFVETR
                     RMVLLK"
BASE COUNT         3364 a         6098 c         6330 g         3037 t
ORIGIN      
        1 tcgacacgcg cgtcgacgat ctgcgcgggc taggcatcta cctcgtggtc gaggaaacgg
       61 aggctttgta tctcctgtgc gcgcgcgatg cgcacgcccg cgttcgcgtg ggcggcgtct
      121 tcgtgccact gcacgagacg acgcaggacc ggtgctcgat ccgcggcgaa gagatggata
      181 tcgaaaccac gcgcgctcag cacgatctgt tcgcgcagac gctgcccgac gccttggtgc
      241 tgtatcgaat cccgcgcccg caggacggga ccgagctcgc gttgttgctg ggcgggtctc
      301 ccggcgacct ggccgccgcg ctggatgccc tcgatgcgcg cccggcgggg gccgcctcgc
      361 tcgagagcga tcgcatcgcg cgctctctcg tgtgtttgcg cttcgacgcg aggcgcgcga
      421 cccgcgacga cggagacgtg cgcgtcgcac gccgctcgcc gggcgcgtgg acgcggggcg
      481 cggtggcggc gctcttggtg gtgggcggtg cggtgggtgt agggctggtg gcgacgcgcg
      541 tcgacttcga gcggcctccg ctcgcgcaca acgacgccga cgccgagcgg accgcgcgcg
      601 cgacccagga gcgcccgacg cccgtcatga cgcgcgacgc gtcggtctcg gaggcgacgg
      661 cggaagtcgc cgccgtcgac gagcgggcgc gcgaggaagc cgaccacccc cgcttcgcgc
      721 tggcgtggga gaagtcgtac tcggagccgg tgacgtcgtc gccgtcgctg ttgggcgacg
      781 gcgtcgtgtt tgggtcgcgc gacgggcgcg tgtacgcggt cgaccgcggg aacggaaagc
      841 agatgtggtc gcacgcggcg gtgggcgggg tgggtgcttc gccgctcgtg cggggcgacg
      901 ccgtcgtggt cgccgactac ggcggcaacg tctaccggct cgcgcgcggc gacggtcgca
      961 cgctgtggaa gcgcgcgctc aaagagcgca tcgtgagcac gccggccgcg accggcgagc
     1021 gggtcgcggt ggggacgtcg cgcggcaacg tgtacgcgct ctcgttcgag accgggcgcg
     1081 tgctgtggaa gttcgcggcg cgtggccaag tgcgcggcgg catcgcgcat gcgggcggca
     1141 ccttcttcgt gccgtcgcac gacggtaggt tgtacgcgct cgccgacgac accgggcgca
     1201 agcgctggtc gctggccctg ggcggcccgg tcgcgtcaac gccgttcacg gacgggacgc
     1261 gcgtggtggt ggggaccgcg cagggaagcg tcgtggcact cgcacatgcc gacggcaagc
     1321 gactgtggtc cttcgcgacg cgcggtccgg tcaactcggc ggtgttgatc cacgacgggc
     1381 gggtgtacgc gggaagcgcg gacgagcacg tgtactgcct cgatgcgggg acgggaaagc
     1441 tgatctggag cttcgagacc tcggggacgg tgctgtcgcg acccttcgtc gacgggaagc
     1501 gcgtggtggt gacgtcgtac gacggatccg tctacgcgct cgacgcgagc gacgggaccc
     1561 tggtggatcg gtatcccacc gagcagtcga tcttctcttc gccggtcgtc gacggcgacc
     1621 gggtctactt cggcaacaac gccgggcgct tcttctgcct caatctgcag gactcttagg
     1681 ctgcctgccg ggcagggttc gccccgagcc ccgccgacgc gccttgcgca tcccggcgtc
     1741 tatttgctac cttgcaacgt aattcacgag agggtttcga cgttctatga gtctgcttca
     1801 gaagctattc aagcccaagt cacagcgtga ttttaagcga cacggggacc tcctcgagca
     1861 agtcaaagcg cgagccgaag agtatcgcgg cttcaccccg gagcagttcc gtgcgaagag
     1921 cgacgagttc cgcctccgtc tccaggaggg agcgaccctc gatgacctgt tgccggaggc
     1981 tttcgcgctg gtcaagtccg cgtgccaggc gctggtcggg acgacgtgga gcgtggtcgg
     2041 tatccccacc aagtgggaaa tggtgccgta cgacgtccag gtcgtgggcg gcatcgtgct
     2101 gcaccagggg aagatcgccg agatggcgac cggcgaaggg aagaccctcg tcgccaccat
     2161 gccgctgtat ctcaacgcgc tcgccgggcg cggcgtacac ttggtcaccg tcaacgatta
     2221 cctcgcgcgc cgcgactccg agtggatggg gaagctcttc gagctcctgg gcatttccgt
     2281 cggcgtgatc cagaacgaca tgcccaacga cctgcgcaag cagatgtacg cgcgcgacat
     2341 tacctacggg accaacaacg agttcggctt cgactacctg cgcgacaaca tgaagacgcg
     2401 cgcggacgac cgcgttcagc gcgggcaccc ctacgccatc gtcgacgaag tcgacagcgt
     2461 tctgatcgac gaagcgcgaa ccccgctcat tatctcgggt ccggtttcgc acagccagtc
     2521 gtccgacttg ttcgcgcgct tgaagcccaa ggtcgagcgg gtggtcgcgc tgcagaatcg
     2581 tttggtcaac gacctggtcg cgttcgccga gaagaacctg gagaacgagg agcaggccga
     2641 cgaagtggcg cgcgcgctgc tcaaggtcaa gcgcggctcg cccaagagca atcgcttcat
     2701 gaagctcatg gcggagccgg gcacggagcg catgatccag cgcatcgaag tcgagttgat
     2761 gcgcgacaag gtgctccacg agctcgacga ggagttgtac tttgcggtcg acgagaaggg
     2821 caactcgatc aacctgaccg agaagggtcg cgaggcgctc tcgcccgagg atcgcgagca
     2881 attcgtgctg cccgacctga gcgaggccat cgccgccatc gacgccgacg agtccctttc
     2941 gcccgccgac cgcgtgcgcg ccaaggacga cgcgcaccgg gcgtacgccg agcgctcgga
     3001 ggcgatccac aacttcagcc agttgttgcg ggcatacacg ctgttccaaa aggacaacga
     3061 atacgtggtc caggaaggcc gcgtgctgat cgtcgacgaa ttcacggggc gcctgatgcc
     3121 ggggcgccgc ttctccgacg ggctccacca ggcgctcgag gccaaggaag gggtcaagat
     3181 cgagggcgag acgcaaaccc tcgccaccat aaccctgcag aactacttcc gcatgtacca
     3241 gaagctggct ggtatgaccg gcaccgccga gaccgaggcg gaagaactgc acaagatcta
     3301 caagctcgac gtccaggtcg taccgaccaa cgagatggtg cggcgcgtcg actacgacga
     3361 cctgatcttc aagaccaagc gcgagaaata caacgcgatc gtggacgagg tggcgcggtt
     3421 acacgagcag aagctcccgg tcctggtggg cacgatcagc gtggaggttt cggagacgtt
     3481 gtcgcggatg ctcaagcgca aggggatcat gcacaacgtc ttgaacgcca aattccacca
     3541 gcaggaagcc gagatcgtgg ccaaggcggg acgcgcgggc gcggtgacca tcgcgaccaa
     3601 catggcgggc cgcggcaccg acatcaagct ggagggctcg gtcgtcaggt gcacggagtg
     3661 cggcatcggg tcggggcgca aggactggca gaccaaggac ggcggcaccg tcgacacggt
     3721 cgcgtgcgcc caggacgtcc cgtgcggcct ccatattata ggcaccgagc gccacgaatc
     3781 gcggcgcatc gaccgccagc tgcgcggccg cgccgggcgc cagggcgacc cgggcgcgtc
     3841 ggtcttttat atctcgctcg aggacgacct catgcggttg ttccgccccg agcgcatcgc
     3901 cggcgtgatg gaccgcctgg gcgtcaagga gggcgaggtc atcacccacc cgctggtgac
     3961 caagagcatc gcgcgttcgc agaagcgcgt cgagaatcac aacttcgaga tccgcaaacg
     4021 gttgatcgat tacgacgacg tgatgaacaa gcagcgcgag gtcatctacg ggcgccgcaa
     4081 cgaggtgatg gacgcacagg aattgaagcc catcatcgag accatcatcg acgactatgt
     4141 cgacaccacg atcgagaaga cggtcgactc gtcgcaggtg ccgggtgccg cggagcgaac
     4201 cgagctgctc ggccagctcg agaacgtgtt catgtacacc ttcgcgcacg attcggacca
     4261 cgcggaaacg gtcgaagagc tccgcgagca cgccaaggcg caagcgcgcg aggcgctgcg
     4321 cgcgcgcgaa gaattgctgg cacgcgactt gaacaacccc gatctggtgc gcgagttcga
     4381 gaagttcgtc ctgctccagg tcatcgacga gaagtggatg gaccacttgc acgagctcga
     4441 ttcgctcaag gagggtatcc actaccgtgc gtacgcccag aaggatccct tggtggagta
     4501 caagcgcgag gccttcggcc tgttcgcgga cttgaacgag cgcatcgacc gcgacgcgct
     4561 ctacgccatg ttccacgcgc gcgtggcggc ggccccccga ccgacggcgg acctcgcgcg
     4621 cgcgcgggcc gtccaccgcg aggcggcccc ggtgctggcg cccggggggc agggcacgag
     4681 cccgctgggg actccatctt cggcgccggc cgcgacgccg gcgcttaatc gcccggccca
     4741 gccgccgctg ggtgccacgg aggcgaccag ccgcgtgctc cggggcgcgg cttcgaccca
     4801 acccgtggtc cgcaccgagg acaaggtggg ccgcaacgac ccgtgcccgt gcgggagcgg
     4861 aaagaaatac aaaaagtgcc atggaataag cagttagccc gctaaccgac ccccctcctt
     4921 gacacctgcg ctaaattgat ggttgccggt tccgccgggg cgggtttatg tttcagggcc
     4981 cgtttcgggg gcgtgcacgg ctcttgcaga gtcaccgccc gaacggaggc aacccgcatg
     5041 tggactaggc tcatggatct ggtgatgctc gtcgcggaga tgagccgcag cgctgaccgg
     5101 cgtccggtcg agctggaccg ggaactcacc cggcgcggct actcgagcga agagatcgag
     5161 cacgcggtgt tctggatttc gtcgcgaccg gaggcgagcc gcgcgatggc ccggcccggg
     5221 cgtgcaatgc gcgtcttgag cgaattcgag cgcatgagtt tgagcacgga ggcgtacgac
     5281 tacttgttcc gcctccagaa tctcgggatc atcgacggac ggcagttcga gacgatcctg
     5341 gcccgcgcca tcccggtcgg cgcggagaag gtacacgtgg aagacgtcaa gggaatcgcg
     5401 tgctcggtga tcttcaaccg cgacacggga gacgtcgacg aggaggcgtt cgatcggttc
     5461 gataccgacg gcgcgccggt ctaaagaaac cggcgcccga cagactcctc ccgcgacgca
     5521 agattcccat cttgcgattt tgtttttagg gaaactatgg ccaacaacgc actggtaatc
     5581 gtcgaatcgc ccgccaaggc gaacaccatc aagaagttcc tgggcaaggg cttcaaggtg
     5641 gtggcctcgg tcgggcacgt gatggacttg ccgaccaacc gtctcggcgt cgatctggac
     5701 aacggattct cccccgagta cgtcgtctcg cgcgggaaga agaagatcct cgaccagatc
     5761 aagaaggacg ccaaggcggc cgaccaggtc tacctcgcgc tcgacttgga tcgcgagggc
     5821 gaggcgatcg cgtggcacat tcgcgaatac atcgcgcccg tgcagcgcaa cgtcaaacgc
     5881 atcatcttca acgagatcac caagggcgcc atcaagagcg ccatcgacga ccccatggac
     5941 gtcgacatga agaaggtcga cgcgcagcag gcgcggcgca tcctcgatcg cttggtgggc
     6001 tacaagatca gccccatcct gtggcagatt ttctactacg gattgagcgc gggccgcgtg
     6061 cagaccgtgg gcttgcgctt ggtgtgcgag cgcgaggccg agatcgaagc gtttgtcgcg
     6121 gaagagtact ggaccatcga gggcgtgctg accacccccg gcggcggcga gcttccggtc
     6181 aagctgatcg agaagaacgg cgagaagatc gagctcaaga accagaaaca gtccgacgcg
     6241 gcgctcgcgg acctcaagaa ggcgtcctac gccgtgacct cggtcgagaa gaaggagcgc
     6301 aagcgcaacc cgcagcctcc ctatatcacg agcaccatgc agcgggacgc ggcgcgccgg
     6361 ctgcgctttt cggccaagaa aatcatgatg ctcgcccagc agctctacga gggcgtggac
     6421 accggcaacg gcgaccgcgt cggcttgatc acctacatgc gtaccgactc ggtgcgtatc
     6481 gccgatcagg ccaaggagtc cgcgcgcgac tacatcgtca agacgtgggg cgaagactac
     6541 agcccggaaa aggcgcgcgc gtacaagacc agtgccaagg cgcaggacgc gcacgaggcc
     6601 attcgcccct cggacgtgga gcgcacgccg gagtcgctca aggccaacct gaccaaggac
     6661 cagtacgcgc tctacgacct gatctggcgc cgcttcctcg cctcccagat ggagagcgcg
     6721 cgttacgaca ccaccgaagt gctggtgggc gcgggcgcgt acacgctcaa ggccaacggc
     6781 cgcgtgatga cgttccccgg cttcctggcg gtctacgagg agagcctggg cgacgagggc
     6841 ggcgagttgc cgccggtcaa ggagaaggac gcgctcgcgg tcaagtcgat cgacggcatc
     6901 cagcacttca ccgaaccgcc accgcgctac accgaggcca ccctcatcaa ggatctcgaa
     6961 gacaagggca tcggccgtcc cagcacgtac gcgaacatcg tgtcgatcat ccaggatcgc
     7021 gagtacgtcg agaaggagga gggccgcctg cgcccgacca cactgggccg ccaggtgtgg
     7081 aagacgctcg agcgcttctt tcccgaagtg ttcgacacgt cgttcaccgc ccaaatggag
     7141 caggagctcg acaaggtcga aggcggtgag ttcacctggc aagcggtggt caaggacttc
     7201 taccagccct tcaaggagtc actcgaacac ctcgacgaca agaaggctcg gctcaagagc
     7261 gagctccagg aagagaccga cgtcaagtgc gagaagtgcg gtcgcaacct catcaagaag
     7321 tggggccgca acggccagtt cctggcctgc ccggcgtacc cggagtgcaa gttctcgcgg
     7381 cccatcgcgg atccgtcggc caacgtcacg gtggaaggcg cctgcccgaa gtgcggctcg
     7441 caactggtgg cgaagagcgg gcgctacggg cgcttcgtcg cctgcgcgcg ctaccccgag
     7501 tgcaagcaca ccgaggcgtt cccgatcgga atgaagtgcc cgcgcgaagg ctgcggcgga
     7561 aagatcgtcg agaagcgcac ccagcgcggc aagacattct atgggtgcag caactaccgc
     7621 gcgcccaagg aaggccaggc acccggctgc gactgggcca gttgggacaa gccggtggcc
     7681 gaggaatgct cgaactgcgg cagcccgtac atggtccaca agtcctcgaa gacgcgcggc
     7741 gacttcctca agtgcccgaa gtgtaaagag gaaatggcga gcgagtagag tcgcgcgcgc
     7801 aagcgcgcta gggtggcgag catgggtgcg gtgacaatcg tcggtggcgg tctggccggt
     7861 tgtgaagctg cactacaact cgccgaccgc ggcttccatg ttcgcctttt cgagatgcgg
     7921 cccgagaccg caaccggcgc ccacaagacc gaccgcctgg ccgagatcgt ctgctccaat
     7981 tccttcaagt cgaccttgct cgacaccgcg tcgggactgc tcaaggccga gatggacgcg
     8041 ctgggctgca ggctgctccc ggtcgcgcgc gagaactcgg tgcccgcggg gcacgcgctc
     8101 ggcatcgacc gcgacctgtt ctcctccgcg gtgacggcgc gcgtcgaggc gcacgccaac
     8161 atcaccatcg agcgccgtcg cgtggactcg ctcgacctcc ccgtgcccgc cgtggtggcc
     8221 accggcccgc tgaccgcaga ggcgctctcg caggcgctgg tatcgcactg ctcacgcgac
     8281 cacttgtact tctacgatgc catcgcgccg tcgatcgacg gcagcaccgt cgacccggcc
     8341 gtgggctatt gggcgagccg gtacggcaag ggcgatgcgg attacctcaa cattcccttc
     8401 agccgcgacg agtaccggcg cttgatcgag ttcgtacgcg gtgccgaata ctcgcagtcg
     8461 cacgccttcg aagaggagaa gtacttcgag gcttgtctgc ccatcgaagt gctggtcgcg
     8521 cgcggcgagg acacgctgcg cttcggcccc atgaagccca aggggctgcc cgacccgcgc
     8581 accggacgcg acccctacgc ggtgctccag cttcgcaagg agagccgcga aggaacgctg
     8641 atgggtctgg tcggattcca gacccgcatg acctgggcct gccagaagga gatgctgcac
     8701 ctgctcccgg ggtgctcgga cgtctcggtg ctgcgcttcg gcaccatcca tcgcaacatc
     8761 ttcctcgaca tccccacgct gtgcgacccg tacctgcgcg accgcgcgcg cccgggactc
     8821 cacttcgcgg gccagatctg cggtgtggaa ggctacgtcg aatcgatcat gagcgcgatc
     8881 atcgtgtcgc tctcgatcgc ggccgcgtcg cgggggcgcg agatgcctcc tatcccgagc
     8941 gagacgatga tcgggtcgct gatggactac gtgcacacgc cgcgcaagaa cttccagccc
     9001 atgaatgcga atttcggcct gcttcccgcc ggcgcgggcg gccggcgcaa ccggcagacg
     9061 cgccaccaag acgccgcgaa ggcggcgatc gccgcgatgg agtcgtaccg ggaccgcaat
     9121 gcctggctct tcgagtaacg ccggccgcct cacggcccgc gcggcgtcct agatttcgct
     9181 taaccgcttt tcgaatcacc ccttatattc ggggcacgcc cggcactcgc cccgcccggc
     9241 gcaccgagtt cctatgctgc tggacaccgc catcgagcga tttcggaccc acctcgcgca
     9301 cgagcgcggg ctgtccccgc ggaccgtcga ggcctacgcg agcgacctcg cgtcgctgtc
     9361 ggcgttcctg gccgagcgtg gaagggatcg tatccaggcc ggctccgtcg acgcgaccgc
     9421 gctgcggtcg tacctcgccg ccgaggtggc gcgcggactc tccggtcgct cgatgatgcg
     9481 acgcgtctcc gccttgcgtt cgttctttcg cttcctcgag aagcgcgggg cgatcgcgtc
     9541 ggatccgacc ctgcatctcg cgcaacgcgt cgcgcgccgc tcggtgccga ccatcgtgag
     9601 cgaggagcgc atcgagctca tgatgggcct gccggacgtg tcctcgccgg cgggtgcacg
     9661 cgaccgcgcg attctcgagt ttctatacgg caccggggtg cgcctgggcg agttggtggc
     9721 actcaaggtt ggcgattttc tgccgctttc ggaccgtgtc gtggtgcacg gcaaagggag
     9781 caagcggcgc gtcgtgccct tcgcgggcag cgcgagagag agcatgctgg cgtactgggc
     9841 gtcgcgcttc gggctttcgc gtgccacgga tgggtcgctg gaggcgtacg ccggagcgcc
     9901 ggcgcttgcc gggcgcgcga agggggacaa agagtcacgc gtgagccggc gtaccgtgca
     9961 gcgcgtggtc ggtcgccact tgcggcgcgt ggcgtcggtc acccaggcct ccccccacgt
    10021 cctgcgccac gccttcgcga cccacatgct cgaccacggc gcggacctgc gcgcggtcca
    10081 ggagttgctg gggcacgaga gcctctcgac cacgcaggtg tacacccacg tctcgatcga
    10141 gcacctgcgc aaggtctatc gaaaggcgca cccacgtgcg tagcacgcgg gcggcgctcg
    10201 ccgttctctt ggcgctcgcc tgcgcggtcg aggaaccgcc ctcgggcggg ccggaagaca
    10261 aggcgccccc ggccgtggtg gagaccacgc cgtcggcgga ctcgacgggc gtggacccgg
    10321 cgagccccat cgcgatcacg ttcggggaga agatgacgcg cgcgcgcatc gagcgattgg
    10381 tgacggtgca gccgcccatc gagatcgagc gcgtgcgctg ggaaggaaac acgctggtaa
    10441 tcgagccgcg cgggggattg cagcgcgaca cgacgtacgt cgtgcgcttg aagccgggtt
    10501 accgcgacag ccacggtgtc accaacaccg cggggcgcga gttcgcgttc gcgaccggcg
    10561 atgcacctct cgacaccgcg cgcatcagcg ggacggtgct gttcaagcgc gagccgtcgg
    10621 gcaaggcgat ggtgcgcgtc ttccgcgttc cacgcgaggc ggattttcgc gccgacgccg
    10681 cgcgccccga ccgcgaggtg gcgacgcggc gggacgggac ctacacgctg ggtcacctgc
    10741 cggccaacga tgcgcgtttc atcgtgatgg cgtacatcga ccagaacggc aatgccgcct
    10801 tcgacaaggc cagcgagccg ttcacggtcc ttcccgacac cctggtgctg acgggcgccg
    10861 tcaccgaggt cgcgggcgtt cagatcggcg ttatcgatcc caatgagccc gggctcgtgc
    10921 gcggcacggt cgccaaccag acgggaatcg acagcttgtt ggccacggtg tcgctgaccg
    10981 cgacgcgcga ctcgactcgc gtgcagtatc tggtgcggtg cgacacctcg ggggccttcg
    11041 agttcgctcg ggtcaagccg ggggggtatc gcctgtgggc gtttctcgac cttcgcgcgg
    11101 acagcgcgcg cggccacttc gactgcggcg acacactgac ctgccccgag ccgtgggcga
    11161 cgctgccgga ttctctttcg gtcgggcccg gcgcgaccgt cgacgtgggc actctcgtct
    11221 tgagacgacg cgacaattag cgaggcgacg atgcgaatcg aattcgtgaa gatgaacgga
    11281 gccgcgaacg acttcatcct ggtggacaat cgccgcgacg acgtgcgact caccggggcg
    11341 cagatcgccc ggctgtgcga ccggcgtcgg ggcgcgggcg cggacggctt cgtgtccatc
    11401 gagggcgggg cggcggcgca agggcgcgac ttcttcatgc gcttctacaa cagcgacggc
    11461 accgaggccg agatgtgcgg caacggcgcg cgctgctcgg cgcgtttcgc cgccgagctg
    11521 ggcctgggga agcgccacga ccacgaggtc gtgctcgatt tcatgaccgg ttcgggcccc
    11581 atcgaggcgc gcgtgcgggg cgcacgcgcc tggatgcgca tgatggacgc cgcccgcatg
    11641 cgccgcggca tcgagacgcg cgtgaagacc ccgctgggtg ccgtgcactt catgaccgtg
    11701 ggcacgcgcc acgcggtggt tccggtcgcg gatgccccgg cgatgacgcc cgccgaggtt
    11761 ttcgagctcg ggcgcacgct tcgctacgac cccgccttcg cgcccgaagg cgccaacgtg
    11821 aacttcgctt cgattggcaa ggacggccgt atttaccttc gcacctacga gaagggtgtc
    11881 gaagccgaga cgttcgcgtg cggaaccggc tcggtcgcgg cctcggtact cttcgcgcac
    11941 gagggtcgtc tcaatagccc cacgcgcatc gtacagcaca gcggcgacga gctgaccgcg
    12001 tccttcgaac tcgcccccac cggcgcggtg aacgtcgcgc tcgaaggccc cgccgaggtg
    12061 aacttccacg ggtgggccga cgtctgacct cttctcgcat gcgaacatgt ctgagcacgc
    12121 ttgcattagc ggcgatcgcg gtttgcctgg ccgggagcgc gcgggcgcag tatcccttcg
    12181 gtaagaacaa ggtcatttat cagggcaaag actggcgcgt cctcgaaaca ccccacgtcg
    12241 acatctatca ctacgccccc gactcgacgc tgatcctgta cctcgcgccg ctggtcgaag
    12301 agaccttcct cgagttcagc gagaccttcc gcctggagtt tcggcgccgg ttgccgttcg
    12361 tcttctatgc cacccactac gacttccagc agaccaacat cctgcccgtg ctgatctcgg
    12421 agtacacggg cggtttcacc gacctcatga agggacgcat cgcggtcccg ttcaacggat
    12481 cgtatgccaa cctgcgtcac gtcgtgcgcc acgagatggt gcacgccttc atgttggaga
    12541 agctccaggt gcacatgcac gagatgggga agtacacctt ctcgcacccg ccgctatggt
    12601 ttgtggaagg aatggcggag tatttcgcca acgcgcccca gaactcccag ggtgagatgt
    12661 tcgtgcgcga cgcgctgctc aacggacgcc tctacgacct cgacgagata tggcgcatcg
    12721 agggctcctt catgatgtac aagcagggcg aagcggtgat caactacatc gcgaccaatt
    12781 tcgggtccga cgccgtgatc cggatcctgg aggcgtggtg gaccgcaccc gagttcacgc
    12841 tggtgctcac caacgcgatc ggaatggacc tctacgagct caacgacgcc ttcatgaagt
    12901 acatgaagcg aaagcactat ccggccgtcc tcacgcgaca gtttgccagc gaccagggcc
    12961 tgcaactcac gcccccacgg tcgttccaca gccgacccgc cggggccttc gtcgacgggc
    13021 ccaatgcgga ctcgaccggc gcgcccatcc aggagaaggt cgtgtactcg ctcaccggtc
    13081 gcgacggggt ggtggtgctg acgcgcggac acgggagcca gcgcgtgacc cccgccaccc
    13141 ccggtgcggc cggggttacg gccgtcgcgg acatgccgtg gaacgagcac gtcgtcgtcg
    13201 ccggcggacg ttcgcgcgag ctggaatcgc tgcctgcgtt tcgcagcaag atcgaggcgc
    13261 tcggccacac cctggtgttc gtctccaaga gcagcgcgcg cgaccgcatc tatttctgga
    13321 gcgcgcgcaa gggcgagagg atcgcgcact tcgaattcga cggtctctcg atcatccagt
    13381 cgcccacggt atcgggcgac cgcaccaaga tggtgttcag cgccatcgac acgcgcggcg
    13441 tcatggacct gtacctcgtc cacctggagg acggtcgcct cgagcgattg accgaggatg
    13501 gtttcagcga ggaagacgcc gactaccacc cgtacgacga cgtggtcctg ttcgcgtcgg
    13561 accgcggcgc gggacagagc aaggaccgca cgcatatcta tcggatggac ctcgagacgc
    13621 gcgagatcgc cgcgctggaa ggcggaccct tcggcgacag caatcccgag tgggcaccgg
    13681 acgggaattc gttcctgttc acgtccgacc gcgacggcgc ggcgaacatc tacctgcgcg
    13741 agggcgacgc ggtcatccag cagaccgacg tcctcacggg tgtcagcgcg ccgtcgttct
    13801 accccgacgg gagcggattc gtggcggtgg tgtacgaggg aggcgagttc cagctctacg
    13861 actttccgat tcgacgccac acgcccgtac ccgcgagcgc acgcccgccc gacgacctgg
    13921 tggttccgtg gacacggtcc gatatgccgg actcggcctt tgtcaccaag ccctacgaga
    13981 cgcgcttctc gatcgacttc atcggcgccg gcgtggccat cgaccccgag gcgggcgacg
    14041 tcggcaacgg aggggcgatc gtcatgaccg acgtgctcgg caaccaccag ctctccttcg
    14101 tgttcgggac tactacggag gagttcaata acttctggga cgacttcaac gcggccttcg
    14161 cgtacttgaa cctctcccat cgcgtcaatt attcgttgag tttctttcac ctcaacacct
    14221 actccgacct gcgcctgttg tcgcagcgcg agaagcgcgt gggaggcgcc ttcgggatca
    14281 gctatccgct cgacaagttc cagcgcatcg agagctcgtt ggtgctgcgg cagatcgaga
    14341 tctcggacct ggaagcgctc attcgggaca gcgagaagcg cagcttcacc gggtccgtct
    14401 tcatgagcta cgtccgcgac aacgcggtgt ggacgttcgg cggccccctc acgggctggc
    14461 gttactacgt cacgggcggg cagacggtgg acttccggga ccgggggttc gacaacgtgc
    14521 tcctgcaggt cgacgctcgg cgctacttca agctggggtc gcgggccgcg ttcgcgctgc
    14581 gctatctgaa ccgcagcgcg tggggcggcg acgagcaggt gttctacctg ggcggccctt
    14641 ggacgctgcg cgggtatccc tacaaccagt tcttcggcaa gaccatccaa ctcgtcagcg
    14701 cggagctgcg ctttcccttg ctcgacgggc tccgcatcgt gctgccgttc ggccccatcg
    14761 agttccccat gttccgcggg tcgctcttct tcgacgcggg catgacggat cgcaacgagt
    14821 tcaagatcct cgacaccgag tggctggggt cggtcgggac cggcatcgag ctcaatctcg
    14881 ggtacgcacc cgtgatgcga gtcaacttta catggccgac cgatttcgat acgatctcca
    14941 gcgacaccgg cttcgagttg ttcattggct acaactacta gcctctccct tcaatccttt
    15001 accgaaggtc gtcacgcatg cgctggtcga agtactacct gccgacgtac aaggaagtcc
    15061 ccaaggacgc ggagatcccg agccaccgcc tcatgctgcg cgcggggatg atccgcatcc
    15121 tcacctcggg ggtgtactcg ttcctcccga tgggataccg cgtgctgcga cgcgtcgcgg
    15181 cgatcgtgcg cgaggagatg gaccgcatcg gttcgcaaga ggtacaaatg ccggtcttgc
    15241 acccggccga gctctacgaa gagaccggcc gcttgaagaa cttcggcgag atcttgttcc
    15301 aattgaagga ccggcgcgcg cgacagttcg cgctcggccc cacgcacgag gaagtcgtaa
    15361 ccgacatcgc gcgcgcggag atcaagtcgt accgccagct cccgcagacg ctctaccaga
    15421 ttcagaccaa gttccgcgac gagttccggc cgcgcttcgg tgtgatgcgc gcgcgcgagt
    15481 tcatgatgaa ggacgcctac agcttccacg cgtccgacga gtcgctgcac gacacctacg
    15541 cggccatggc gggggcctac cgccgcatcc tcgagcggtg cgggctcgaa ggcgtgcagg
    15601 tgcaggcgga gtcgggggcg atcggcggcg acatcaacga ggagttcatc gtgctggcgg
    15661 atgcgggcga gagcgtcatg tttcgctgtc cgtcgtgcgg gtacgcggcc aacgacgaac
    15721 gcgcggtgag ctcgggtgcc gccacattgg agaaagagcc gtccgcggag atggagaagg
    15781 ttgcgacccc gggccagcac acggtcgacg aagtgagcgc gttcttcaag gtgtcgaagc
    15841 agcgcctcgt gaaaacgctc ctgtacaaga gcaagtggcg cgacgtcgcc gtgctcgtcc
    15901 cgggcgatcg cgagttgaat gaggtcaagc tcgtgaaagc gctcgacgac cccgaggcgc
    15961 ggccgatgac gcccgaggaa gtcgaggcgc tcacgggggc gccggtcgga ttcgcgggtc
    16021 cggtgggctt gaaggcgatc ctgatcgccg acgatctgtt gaaggactac gacggcatga
    16081 tcgtcggggc caacgcggcc gacgcgcacc tgcgcggtgt gaagatgggg cgcgacttca
    16141 agcccgagcg cacggcaccc ctttccaccg tggtggcggg agaccgttgc gcgagctgcg
    16201 ggaagggcga gttggcgctc cagcgcggcg tcgagatcgg ccacatcttc aagctcgacg
    16261 tgaagtacag caaggccatg cacgccacct tcctggaccc ggaagggcag gaaaggccgt
    16321 tcgtcatggg ctgctacggc ttcggtgtct cgcgcgcggt ggcggccgcc atcgagcagc
    16381 actacgacga gaagggcatt cgctggccga aggcgctgac accgttccac gtcgtcgtga
    16441 cacctctcaa tctgaacgac gagggcacgg tgaagacgat ggagcgcgtg tacgctgatc
    16501 tgcaggcggc tggcttcgac gtcttgctgg acgaccgcga cctgcgcccc ggtcccaagt
    16561 tcaaggacgc ggagctgatc ggcgtgccga tgcgcgtcac cctgggcgag cgcaacctca
    16621 aagacggaaa ctgcgagctc tattaccggc tcgaggacaa gaccgacatc gtggccttgg
    16681 ccgatgtgac atcgaagatc cgcagctatt acgcgaacta gcgggctccc ggacgcgcac
    16741 gagagccgct agcctgcgac ctacttcagc aacaccattc gacgcgtctc cacgaaggtc
    16801 ccggacgtca ttcgatacag atagacgccg gaagccaccg gcgtgccgct cgcgttgcgc
    16861 ccgtcccaat ccacccggtg cgtgccggcg gggcgcgggc cgtcgaccag tgtgcgcacc
    16921 gcacgccccg tcacatcata gatctcgatc gaaacgtgcg cgccgcccgc gggcacgtcg
    16981 taagcgatcg tcgtgttggg gttgaacggg ttggggacgt tctggtggag ggcgagtacg
    17041 acgggtgcgt cacccacggc ggtcggcact ggcgcaacca acgcgaacgg cgagaagtgg
    17101 tcggtgacgc cgcagatggt gttgccgatg atctcgagcg acgtggtgat atccacccag
    17161 gcgtcgggat tcagttccgt gtcccagtgg agcaaccgga ggtcggcctc ggggacggtg
    17221 agcgtcccct cgtcataggc gatgcaaatc tcgacgttgc ccagaaacgt cgccgacgtg
    17281 gaaacgtcgt agtaggtgcc gttcgcgagt gtgaacagcc cggtgaccgc cggacccagg
    17341 cttccggtgg tcagcgtggt tgcgcctggt tggtcgatgt aattgaacgt gagtgttacc
    17401 ggggtggtgc ccgtgacgac gtcttcgggc gtgacgacga cgtcgaatcc ggccggcgtg
    17461 ttggcgatga agttcgcgac gcgcacattg tcgatcaagg gcgcgttggt gtgacaggcg
    17521 ccgctgccca cggaaccgca ccaaatgccg cacaggtcgc gcaccccgat cgcaacctgg
    17581 atatcggtgg caccgccgtc gatgagcggc gcgatctcga acgcgctgac gtgccactgc
    17641 gcggtaggac cgtagtagtt gattccggag gccctccagt cgttccactg gcccccgacg
    17701 cgagaccgga cgcgatactc gtaggtcacc aagttgtcga gcgggaggta tcggtacacg
    17761 tcgaacgcca gcgtcagcga cgtgaccggt tgggccagcg gcgcgagcga gatcacgggg
    17821 gattggatct cgttccgtat gaacgatccg tcgctcccga acggtaccac ggcctgcccc
    17881 gggtggcctc cgcacgtgta atcgtccggg gaattcttga agaagctcca tacgtggctc
    17941 gggttgacga cgttggggtc ttcctgcaat acggagcccc cattgatgag ttccgcgtag
    18001 tcacccttgg ccggaaccgg ggaagcaaac cactcgccgt cgagagtggt cagggctccc
    18061 tccgcttcgg cttcgaattg ctgatagctg acgatcccgg tggcgtcctc gaccaggatg
    18121 tcgtcgatta ccgccgcgcc cagcgaattg tacccgccgt cctcgtcgga gtagacggcg
    18181 tccgaccgca cgcgaaaacg aaactcgacg gtcccgttgt gctcgctcga aaggacggag
    18241 tactcgaagg gggcgtaatc gaggccggtg aacgaatcga gggtctgcca ggtcgacgtg
    18301 agactccggt actggaggtc gatgaagtcg tagctcggct cgaggtcgaa gcccaagagg
    18361 aacgtcagcg tgacgtgtcc cgtcacggaa agtgcaaccg agctaaagcg ctgatcccag
    18421 ctgttgccgt aaccaggcag cgtcaaatag tcgcacactt cgggtaccgc cgggcaagcc
    18481 cgcgcgccac accataatga cttggtgccc gccaagggac ccattccggg aatggccggg
    18541 ttgaagtcct cgacgtggaa gaacgtgccg agctgagagc tgcgatcgta ggtcgtccaa
    18601 ccttgcgcgt cggggccgcc catcccgtct tcgaaggtcc acaagccgag cacggtgacg
    18661 tcgtcgttcg actggaaccg gtcgcgcggg ccgaggatct ccgagagcgg ccatccgggc
    18721 gcgagcgccg tcgtgggcgt gcgccggggc tcttgcccgg ccgcgaccga cgtcgcgacg
    18781 gtcgtgccga gaaccgcgat caggatcagg ggaatacatc gcatgcgat
//
LOCUS       DASPGK010000169        17294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_44326, whole genome shotgun sequence.
ACCESSION   DASPGK010000169 DASPGK010000000
VERSION     DASPGK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44326"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1217
                     /locus_tag="VEC56_04845"
     CDS             129..1217
                     /locus_tag="VEC56_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV13512.1"
                     /translation="MALEGNLTSFKIEEILQLIAVQQKTGMLSVVANDKSAVLFFRDG
                     KITTTRDRRSKTRDPFREYLTRYGCLGREDLTRITQIIAQSKLDLLDILNSEGIFDEK
                     ALARHWRNHIQETLHDVLTWDQCTYKFVSGEEIVAGVKSLGEFAVEPMLMESMRRIDE
                     FPALLQLFPAQGITITATGKVPTGEEPMSDNENLVLSLLKTARSMRDVIARANMPTYD
                     AYESLKLLKEKGLIVIKEDARDGAVGEARISGQVVRRKRGNPMLLVAASFVFFACVVV
                     GVWQKSDEVALVARDGVLRSDPASRARVEQRVRWLLEVYRAEHGVYPESLGGLAEGGV
                     ANRSIGERAATYRFEYRLTRDGAGYTLL"
     gene            1262..2227
                     /locus_tag="VEC56_04850"
     CDS             1262..2227
                     /locus_tag="VEC56_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXV13513.1"
                     /translation="MFLRAGDFVFVLRPLILVPAWSFYALGVHLAPVRARVELFSVVV
                     QPGFWCMTALLASAYVINQIFDQSSDRCNSKGLFLTHGVFRTRSMIAIGLACFLGASW
                     LFQKVEGAQRIPLAASMLVAFAYSLPPIRLCARRWLDLLANAIGFGGLAFAAGAGGVS
                     DYALAAFLDAAPWMLLVGAVFLHTTILDVDGDAAAGKRTTTVAIGVARSAHLATVLAA
                     LAAAMLGWRYFEKAGSLVAMLVAALAFVVVAIANVAVARAEKLDPALRAAARNRASAR
                     VVQSITALIALWACVRDPALLLLFVPLAVAARFYYRARFSIRYPG"
     gene            complement(2224..3117)
                     /locus_tag="VEC56_04855"
     CDS             complement(2224..3117)
                     /locus_tag="VEC56_04855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV13514.1"
                     /translation="MRALVKADSLARMLIPIALALGIPLAMHATWRVRYGYPPDHVPR
                     VLCFHKLSRRFLFEGTWTTPARFAAYIDRLRARGYTFIDEAQYLAALDAPPADRSTQI
                     LLTFDDGYTEVREVAWPLLSERGIPFHVFLVTDYAGRANDWDLSLGRPPFRHLDWAAV
                     REMSGRGVTFGSHTASHADLTRAPRARVHQDLERSRKAIEDATGKTARTLSYPFGRYG
                     SASQEAARAVGFDAAFSLYPSHRNATIDRFALRRDAVYIIDPVWAIETKLRPNPLYGL
                     EEMKCRAINAVAVLTPLLKPR"
     gene            3162..4346
                     /locus_tag="VEC56_04860"
     CDS             3162..4346
                     /locus_tag="VEC56_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV13515.1"
                     /translation="MIQPAKRIQKTGRYVFEEIARMKREDTRPPEDIFDLGVGSPDQP
                     PDPAIIEMMAKQLRARPFENHRYSSFDGAPEFRRAVANWYKRRFNVSVDPDKEVLPLI
                     GSKDGISKLMLAYLDPGDTIVVATPCYPAYLGAAKIVEANVVELPLKPENNYLPDFAA
                     VPHTVWEHAKFLFLNYPNNPVGAAAPLSMYKEAVALAEKFDFIVVSDLAYSELALEPN
                     VPTPSILQVPEAADVAIEFHSFSKSYNMAGWRLGYVLGNREVISNLVKIKSNMDFAVF
                     LAIQRTGADILDSGIDFAEAQRKRYRARRDLFIEGFGKLGWRLTPPPAAMYIWDRVPK
                     RYADATDFAKEFFKATGVIVSPGSAFGSHSKEFMRISMVVDEQKIERLLAKVKASGFR
                     FD"
     gene            complement(4318..4761)
                     /locus_tag="VEC56_04865"
     CDS             complement(4318..4761)
                     /locus_tag="VEC56_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13516.1"
                     /translation="MGASVRIAAALLLIGIVAACDEDENPVDWDDNRAPILDAQPDTS
                     VAYGDTLHLRASARDPDGDRLAYSMAVFASLSEVRTGYVAYARIDPGTGAFWFFPNPR
                     DIPDRSFQFSVQDGRGGQDTVDFTVTVTTTELRATASSRTGTRSP"
     gene            complement(4771..6021)
                     /locus_tag="VEC56_04870"
     CDS             complement(4771..6021)
                     /locus_tag="VEC56_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV13517.1"
                     /translation="MTPARVANKIVLPSGLTILHEKNPISKAFCIGVWTRTGARDESP
                     REVGLCHFMEHMLFKGTNRRSAKQISTDIEKVGGSLDAFTTKDTMCVYGQVLESKRDV
                     AIDLIADMLTESRFAEEQVALERKVVVEEIGDVDDAPEDLIHELFAAEMYPQHPLGRP
                     ILGFRKTVAAFKRNDLVRYAHRAFRSSNLVVAVYGNIDETELIRICESRFAFPKGTLS
                     RDGTRFPAPLARRRHVRRKLHQQHVCMGARTFSFLDERRYALMVLNAALGGGMSSRLF
                     QKIREEMGLAYSVYTYVDHSRDTGMFAAYMAVNPVAAGRAIDAMTRELEAVRTGGLTR
                     TELRDIKDHIKGRILLGLETSASRMMRLARNEISYGRQVPERELLKKLAAVKMADVNA
                     VAREVLDTDRYVMVSLGPSGSGLN"
     gene            complement(6120..8291)
                     /gene="pnp"
                     /locus_tag="VEC56_04875"
     CDS             complement(6120..8291)
                     /gene="pnp"
                     /locus_tag="VEC56_04875"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXV13518.1"
                     /translation="MKRIVNGQPFSIETGRMAKQADGSVVVRMGDTMVLVAVVAAQQK
                     TDRDFFPLTVEYREKFYASGRIPGGFFKREGRPGERETLTSRLIDRPIRPLFDKHFMC
                     ETQVIATPISYDGVNAPDVISIVGASAALALSDIPFQDIVSGVRVGRIDGQFIVNPTT
                     EQNEASDLDVIIAGTDTGVMMVEGGCSEVSEEDMLAAVRFGHEEIKRLNALQRELVSL
                     FGGKKKRVVEVPERLAELDAKIAKEFSDRIGQALFIAKKLDRQDALKTIQKDALAKYE
                     VDYPEKGAYVALVLEEIEKKLMRGMILDKGKRLDGRGSTDIRPITCEVGVLPRAHGSA
                     LFTRGETQSLVTTTLGTTDDEQMLDTIEGESWKRYMLHYNFPAFSVGEVGRFGGPGRR
                     EIGHGALAERALRPMIPPKDEFPYTIRIVSEILESNGSSSMASVCGGSLALMDGGIPI
                     KKPVAGIAMGLVIEDDRVAVLSDILGAEDHLGDMDFKVTGTAEGITAFQLDTKIAGIS
                     DAVMMKALAQAKTGRMHILQEMNKAIAQARADISPFAPKVTTIHIPVDKIREIIGPGG
                     KVVKRIQEESGAKIEIEDDGTVRVIAVNTESAEIALGRIKEITAEPEVGKVYEGEVRS
                     IMPFGAFVQIMPGRDGLLHISEIAHRRLERVEDELQMGEIVKVKVLEVQPDGKVRLSR
                     KVLLEKPEGYQEEERSERPHRERSDRDHGRGGGGRRHSSRR"
     gene            complement(8408..8677)
                     /gene="rpsO"
                     /locus_tag="VEC56_04880"
     CDS             complement(8408..8677)
                     /gene="rpsO"
                     /locus_tag="VEC56_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXV13519.1"
                     /translation="MVMTPQQKSDIIAKYKLHDKDSGSAEVQIALLTERIRMLTEHFQ
                     VHKKDHASRRGLLKLVGRRRKLLDYLKNQKIDSYREMIKQLNLRK"
     gene            complement(8803..9753)
                     /gene="ribF"
                     /locus_tag="VEC56_04885"
     CDS             complement(8803..9753)
                     /gene="ribF"
                     /locus_tag="VEC56_04885"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HXV13520.1"
                     /translation="MRPLAVARSLEELRALAPRDSAVTIGVFDGVHLGHRKILEELVS
                     RRESGRVASAWAITFDPHPLVVTHSREAPPILTTVAERIELLSKFGLDGVFVLPFDEA
                     TARIEYRDFIQRYFMDAMDMREVVMGYDCHFGHQREGSPERVAEEGVRRGFAVTVIPP
                     VYVDGEKISSTNIRRALQAADLERANHLMGHAYLVRGTVATGQGRGRDLGFPTANVAV
                     THPHKQWPSSGVYAVRVGWRGRVYDGMMNIGRSPTIKRVDHDEMEVHVFDFDHHIYGD
                     ELSVHCEAYLRAEARFPTVTALIEQLNEDRRAARERLARG"
     gene            complement(9758..10735)
                     /gene="truB"
                     /locus_tag="VEC56_04890"
     CDS             complement(9758..10735)
                     /gene="truB"
                     /locus_tag="VEC56_04890"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXV13521.1"
                     /translation="MTNRFDNRVFVIDKVPGPTSFEIVAALRRVSGLRRVGHAGTLDA
                     LARGVLLVCTGHATRAVEHFMNLEKEYRFDVRLGVETTTLDAEGDVVREAPVPPLTIE
                     EMRAAAAQFVGDYVMVPPTFSALKLGGRRFSDLARAGEPAEASPRTVRVHAFDVVSVE
                     LPIVHCVVRCSRGTYVRALARDLGAKLGLPASIASLARSRIGNYRVEDAVSSECLVPE
                     RADEVIGLDLGEALSFLPGVVVSTRAKRALLFGAAPLRNDVVETVGAPTIGAIAGPLR
                     ILDEEGTLLAIGTRGGDHPRDPLRVCDSFRLFVEASPGGTGAGAARVSR"
     gene            complement(10732..11112)
                     /gene="rbfA"
                     /locus_tag="VEC56_04895"
     CDS             complement(10732..11112)
                     /gene="rbfA"
                     /locus_tag="VEC56_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXV13522.1"
                     /translation="MTRPNYRIERVNELIKEVLSELVLTEIKDPRVGLITITGVRVTN
                     DLSSAKVLFSVMGDEAQRAMTLKTLKAASGFLRRVLVRHMDVRVAPELRWVYDDSLDR
                     AFRIEQALRETGTIPNDEPEPKDE"
     gene            complement(11113..11394)
                     /locus_tag="VEC56_04900"
     CDS             complement(11113..11394)
                     /locus_tag="VEC56_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXV13523.1"
                     /translation="MLVKLLTVDLLIPGSSSLKEKRFVLKSLKARLRSRFNVSVAEID
                     YQDKWQRCLIAVAIVGSDRESVDGTCGQVRRFIEGDHRVELADALEEFR"
     gene            complement(11435..13975)
                     /gene="infB"
                     /locus_tag="VEC56_04905"
     CDS             complement(11435..13975)
                     /gene="infB"
                     /locus_tag="VEC56_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXV13524.1"
                     /translation="MRISELAKKYNVTNDELVALLKNAGYQVASAGSAVDYDMLTALD
                     RHFSRSGASAKKGKGKTTSKASKAAAAASADTAKAKVKLRKAKGPEPEPVVEAPAKEK
                     PKVKLAKAAKPAAEPAPSVEPKAEAKAEVKEAKSAKKSSSVATAPAAAKTSKRAEAEA
                     EKPALKVAPAPKAPPKPKKLTPLDELAKAAVEPLIAIEPDTPADEVEEWVQATEEHKR
                     HGHVHAKTSDAEQVRESVRRTLAKMETTRKTKRRKTRAATVAPVDLPPVRVQEGATPA
                     DLGYALGVSADDVLSRMSQLDLQADASAALDRDTIELVAEELGRRVDVEASFGETQLK
                     LDAQVDPTKLVARAPVVTVMGHVDHGKTSILDYIRKANVAAGEAGGITQHVGAYEVTT
                     PNGKITFIDTPGHEAFTAMRARGAKSTDIVVLVVAADDGVMPQTIEAINHVRAAKVPM
                     IVAMNKVDLPTANPAGLMQQLAQQSVLVEGYGGDVVSVEVSAKTGKNIDKLLEMILLQ
                     AELAELKADPTARAQGVVVEVRKEEGRGILITVLVQQGTLRIGDVFVVGNEYGKVRSL
                     FDHAGRALREAGPSTPVVVLGADGMPEAGDQFIAVKNEREAREVAQKRQENLRAKGLE
                     PTKALTLEELYAQIQGGAAKELNVVLKADTNGSLEALRDSLLPLNIEGIKINLVHGGV
                     GGVSESDVLLAQSTNAVIIAFNTKVTPKAKDIAKLKGIEIRSYKIIYEAIEDVDKALK
                     GMLEPVFVERVLGRAEVRKIFRVSKLGTIAGCMVVEGTIQRNASARVIRNGEVIAKGK
                     IGSLKRFQDDAREVAQNFECGIGVSGFDDFTERDIIEAFVVEEKARVF"
     gene            complement(13976..15550)
                     /gene="nusA"
                     /locus_tag="VEC56_04910"
     CDS             complement(13976..15550)
                     /gene="nusA"
                     /locus_tag="VEC56_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXV13525.1"
                     /translation="MSIHFAEAFNQLIRERRLDRAILMDTLTQGFASAVRRKYGPNAE
                     VRIEDDGHGSIELFLVKNVVDAVEDPSIQISVVDAQEYAEGAEAGDAIEINLPFEEFG
                     RNAIQVTKQILMQKIREFERDRVFEEYKDRVNEIVSGSVQQIDRTGILVNLGSAEAFM
                     PKKDQIRRERYQQGSSIRAVVMKVDKEAKGPMITLSRTSPEFLKALFIQEVPEIYEGL
                     IEIKSIAREAGGRSKIAVYSRDDRVDAVGACVGMKGSRVQAVVNELSGERIDIVPWSD
                     DVQTFVSRALSPAKITQVRPDIETKTVTVVVEEDQLSLAIGKEGQNVRLASRLTGWQI
                     ELVSSRELEQRQRLQEHLLMPIEEMVGVSDKMAEKLREAGINTVQKLIKTPVGSLLTI
                     PGFGERTVERLMETAQGTVKELEKALEELIRKENEDREEKKRQEKPLFDESMLEAETE
                     EPKKETKKLTAAQLFRDPAEVEAEAAAEPASTEPAEAPAETTAETPAEISAEPTAEAT
                     GDGDAESNPEAEDTKD"
     gene            complement(15551..16066)
                     /gene="rimP"
                     /locus_tag="VEC56_04915"
     CDS             complement(15551..16066)
                     /gene="rimP"
                     /locus_tag="VEC56_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXV13526.1"
                     /translation="MTEQELEKLIVPELETLGFECVKLEVVGGPRHPVVRLFIDKPGG
                     VTVGECALVSRTLGLVFDREDPFPGSFLLEVSSPGSNRPLVTEAHFQRFVGHEAKVVV
                     ERAGARATHIGRIRSCVNGALTLDTDAGEVAVGLREVVRANLINQEYKIDKKREKHRR
                     PPNWEGRGGEE"
     gene            complement(16331..>17294)
                     /locus_tag="VEC56_04920"
     CDS             complement(16331..>17294)
                     /locus_tag="VEC56_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXV13527.1"
                     /translation="QLLKTMPRGEALLRRARAATLASAAALAAMAAGCGASEPPAGSV
                     DSVMVHVERQLSFGPRVPGTPARDAAARYLARALERYGARVSLQSFEVEDPYELRRLR
                     LINVVGSFAPERTRRIMLCAHYDSRPWADQERDSTLWTEPIPGAVDGATGVAVLLEVA
                     RLVGLASPGEIGVDVVLFDGEDYGKRGNIDHYLLGSKHFVMNLNGYRPQGAILLDMVG
                     GKGTRIRREPFSKEHARAFLDHVFARAAALRLDYFEAADGAPMIDDHVPLLQAGIPAI
                     DLFGYDYAAWHTLGDDLSQVDPAKLSQVLVLLRDLLYDRPFPAE"
BASE COUNT         2701 a         5818 c         5619 g         3156 t
ORIGIN      
        1 tggcgtacat ggtcgcgctg ctgtcgcgcc agctctacga ggcgcgcaac acgcgcacgg
       61 tgacggttcg cggcaccaat acggaaagcg actcggcgtc gcagtcggca taaggcaggt
      121 gagacgacat ggccctcgag ggaaatctta ctagtttcaa gatcgaagag attctgcagc
      181 tcatcgcggt gcagcagaag acgggcatgt tgtcggtggt cgccaacgac aagtcggccg
      241 tgctcttttt ccgcgacgga aagataacga ccacgcgcga ccgtcgcagc aagacgcgcg
      301 accccttccg cgagtacctc acgcgctacg gatgcttggg gcgcgaggac ctgacgcgta
      361 tcacgcagat catcgcgcag tccaagctgg acctgctcga catcctgaac tccgagggta
      421 tcttcgacga gaaggcgctc gcgcgacatt ggcgcaacca catccaggag acgctccacg
      481 acgtcctcac gtgggaccaa tgcacctaca agttcgtctc cggcgaggaa attgtcgcgg
      541 gtgtcaagag cctgggagag ttcgcggtcg agcccatgct catggagagc atgcgccgta
      601 tcgacgagtt cccggccctc ctgcagctgt tccccgccca gggcatcacg atcacggcca
      661 cgggcaaggt tcccacgggc gaggagccca tgagcgacaa cgagaacctc gtcctgtcgc
      721 tcttgaagac cgcgcgttcc atgcgcgacg tcatcgcccg cgccaacatg cccacctacg
      781 acgcctacga gtccttgaag ctcctcaagg agaaggggct catcgtcatc aaggaagacg
      841 cccgcgacgg agcggtcggc gaagcccgca tcagcggcca ggtcgtgcga cgcaaacgcg
      901 gcaacccgat gctcctggtg gccgcatcct tcgtgttctt cgcctgcgtc gtcgtgggcg
      961 tgtggcagaa gtcggacgag gtggcgctgg tcgcgcgcga cggcgtgctg cgcagcgacc
     1021 cggcttcccg cgcccgcgtc gaacagcgcg tgcgctggct cctggaagtc taccgcgccg
     1081 agcacggcgt ttaccccgag tcgctcgggg gcctcgcgga aggcggagtg gccaaccggt
     1141 ccatcggcga gcgcgcggcg acctatcgct tcgagtatcg attgacgcgc gacggggcgg
     1201 gttatacgct cctctagttt tcgcgcacac cctctcatcc tcccgtatcg acgccgcgcg
     1261 cgtgttcctg cgcgccggcg atttcgtgtt cgtgttgcgc cccctcatcc tggtgccggc
     1321 ctggagcttc tacgcgctcg gcgtccacct cgcccccgtg cgggcgcgcg tcgagctctt
     1381 ctcggtggtc gtgcagcccg ggttctggtg catgacggcg ttgttggcca gcgcctacgt
     1441 catcaatcag atcttcgatc agtccagcga ccggtgtaac agcaaggggc tctttctcac
     1501 ccacggcgtg tttcgcaccc gcagcatgat cgcgatcggg ctcgcgtgct tcctgggtgc
     1561 gtcgtggctg ttccagaagg tggaaggcgc gcagcgcatt ccgctcgccg cctccatgtt
     1621 ggtcgcgttc gcgtactcgc tgccgccgat tcgtctgtgc gcgcgccggt ggctcgactt
     1681 gctggccaac gcgatcggct tcggcggtct cgccttcgcc gccggcgcgg gcggcgtgtc
     1741 cgactacgcg ctggccgcct tcctcgacgc cgccccgtgg atgctgctcg tgggcgccgt
     1801 cttcctccac accaccatcc tggacgtcga cggcgacgcc gcggccggca agcgcaccac
     1861 cacggtcgcg atcggggtgg cgcggtcggc gcacctggcg accgtcctgg cggctctggc
     1921 ggcggcaatg ctgggctggc gttacttcga gaaggccggt tcgctggtcg cgatgctcgt
     1981 ggccgcgctc gccttcgtcg tcgtggcgat cgcgaacgtc gccgtcgcac gcgccgagaa
     2041 gctcgacccc gcgttgcgcg ccgcggcgcg caatcgcgcg agcgcccgcg tcgtgcagtc
     2101 gatcacggcc ctgatcgcgc tgtgggcatg cgtgcgcgac ccggccctgc tgctgctctt
     2161 cgtgccgctg gcggtggccg cgcgcttcta ctaccgcgcg cgcttctcga ttcgctaccc
     2221 gggttagcgc ggctttagga gcggggtcag caccgcgacc gcgttgatgg cgcggcactt
     2281 catctcttcc aatccataga gcggattcgg gcgcagcttg gtttcgatcg cccacaccgg
     2341 atcgatgatg tagaccgcgt cgcgccggag cgcgaagcga tcgatggtcg cgttgcggtg
     2401 cgagggatac aacgagaacg cggcgtcgaa accaaccgcg cgggcagcct cttgggaggc
     2461 actgccatac cgtccgaacg gatacgacag ggtgcgcgcc gtcttccccg tcgcgtcctc
     2521 gatcgctttg cgcgagcgct cgaggtcttg gtggacgcgc gcccggggcg cgcgcgtgag
     2581 gtcggcgtgc gaggccgtgt gcgagccgaa ggtgacgccg cgccccgaca tctcgcgcac
     2641 cgcggcccag tcgaggtgac gaaagggcgg gcgccccagc gagaggtccc agtcgttcgc
     2701 gcgccccgcg tagtcggtga ccaggaagac gtggaacggg atgccgcgtt ccgacagcag
     2761 cggccacgcg acctcgcgca cctcggtgta gccgtcgtcg aacgtgagca ggatctgcgt
     2821 gctgcggtcg gccgggggcg catcgagtgc cgcgaggtac tgcgcctcgt cgatgaaggt
     2881 gtagccgcgc gcgcgcagac ggtcgatgta tgcggcgaag cgcgcgggcg tggtccaggt
     2941 accttcgaag agaaagcgcc gcgagagttt gtggaagcac agcacgcgcg ggacgtggtc
     3001 gggcgggtag ccgtagcgca cgcgccacgt cgcgtgcatc gcgagcggta tgcccagcgc
     3061 caacgcgatc ggtatcagca tgcgtgcgag actatcggct ttgacgagcg cgcgcaaacc
     3121 tgataatctc gttgcttctt caaacggagg ttctcgccag tatgatccag ccggccaagc
     3181 gcatccagaa aacgggccgc tacgtgttcg aggaaatcgc gcgcatgaag cgcgaagaca
     3241 cccgcccgcc cgaggacatt ttcgacctcg gcgtgggcag ccccgaccag ccccccgacc
     3301 ccgccatcat cgagatgatg gccaagcagc tgcgcgcgcg gcccttcgag aaccaccgct
     3361 actcgtcctt cgacggcgcg cccgagttcc gccgcgcggt cgcgaattgg tacaagcgcc
     3421 gtttcaacgt ctccgtcgac cccgacaaag aggtcctgcc gctgatcggg tcgaaggacg
     3481 gcatctcgaa gctcatgctc gcctacttgg atccgggcga caccatcgtg gtcgcgaccc
     3541 cgtgctaccc cgcgtacctg ggtgcggcga agatcgtcga agccaacgtg gtcgagctgc
     3601 cgctaaagcc ggagaacaac tacctgcccg atttcgccgc cgttccgcac actgtctggg
     3661 agcacgcgaa gttcctgttt ctcaattacc ccaacaaccc cgtgggtgcg gctgcgcctc
     3721 tctccatgta caaggaggcc gtcgcgctcg cggagaagtt cgacttcatc gtggtgtcgg
     3781 atctcgcgta ctccgagctg gcgctcgagc ccaacgtccc gacaccgtcg attctgcagg
     3841 tccccgaagc cgccgacgtg gccatcgagt tccattcgtt ctcgaagagc tacaacatgg
     3901 ccggctggcg cctgggctac gtcctgggca accgcgaggt gatctcgaac ctcgtaaaaa
     3961 tcaagtcgaa catggatttc gccgtgttcc tcgccatcca gcgcaccggc gccgacatcc
     4021 tcgacagcgg catcgacttc gccgaagcgc agcgcaagcg ctaccgcgcg cggcgcgacc
     4081 tgttcatcga aggattcggg aagctgggct ggagactcac acccccgccg gccgcgatgt
     4141 acatctggga ccgcgtgccc aagcgctacg ccgacgcgac cgacttcgcg aaggagttct
     4201 tcaaagcgac gggcgtgatc gtgtcgccgg gctccgcctt cggatcgcac tccaaggagt
     4261 tcatgcgcat ctcgatggtg gtcgacgagc agaagatcga gcggctcttg gcgaaggtca
     4321 aggcgagcgg gttccggttc gactagacgc cgtcgcgcga agctcggtcg tcgtcacggt
     4381 gaccgtaaag tcgacggtgt cttggcctcc gcgtccgtcc tgcaccgaga actgaaacga
     4441 tcgatcggga atatcgcgcg ggttgggaaa gaaccagaac gcgcccgttc caggatcgat
     4501 gcgcgcgtac gccacgtaac ccgttcggac ttcagacagg ctcgcgaaaa cagccattga
     4561 gtacgccagc cgatctccat cggggtcgcg cgcggatgcc ctcaagtgca gcgtatcgcc
     4621 gtaggcgacg gaagtgtcag gttgggcgtc aagaatgggt gcccggttgt cgtcccagtc
     4681 gaccggattc tcgtcttcgt cgcacgcggc aacgatcccg attaacagca aagccgccgc
     4741 gattcgaacg ctcgcgccca ccagtgcctc ctagttcaac cccgacccgg acggtcccag
     4801 gctcaccatc acgtagcggt cggtgtcgag cacttcgcgc gcgaccgcgt tcacgtcggc
     4861 catcttgacc gcggcgagct tcttcaagag ctcgcgctcc ggcacctgcc gcccgtagct
     4921 gatctcgttg cgcgccagcc gcatcatgcg cgaggccgac gtctccagcc ccaagagaat
     4981 cctccccttg atatggtcct tgatgtcgcg cagctcggtg cgggtgagcc cgcccgtgcg
     5041 caccgcctcc agctcgcgcg tcatggcgtc gatggcgcgc ccggcagcca cgggattgac
     5101 cgccatgtag gccgcgaaca tgccggtgtc gcgtgagtga tccacgtacg tgtacacgct
     5161 gtacgcgagc cccatctcct cgcggatctt ctggaacaac cgcgacgaca tgcccccgcc
     5221 cagggccgcg ttcagcacca tcagcgcgta gcggcgctcg tccaggaacg agaacgtgcg
     5281 cgcacccatg cacacgtgct gctggtggag cttgcggcgc acgtggcgtc ggcgcgcgag
     5341 cggcgccggg aagcgcgtgc cgtcgcgcga gagagtcccc ttcgggaacg cgaagcgcga
     5401 ttcgcagatc ctgatgagtt cggtctcgtc gatgtttccg tagaccgcga ccaccaagtt
     5461 cgaggagcgg aacgcgcggt gtgcatagcg cacgaggtcg ttgcgcttga acgccgccac
     5521 ggtcttgcga aagccgagga tggggcgtcc gagcgggtgt tgcgggtaca tctccgcggc
     5581 gaacagctcg tggatcaggt cctcgggggc gtcgtcgacg tcaccgatct cctcgaccac
     5641 gaccttgcgc tccagcgcca cctgctcctc cgcgaagcgg gattccgtca gcatgtcggc
     5701 gatcaggtcg atggcgacgt cgcgcttgct ctcgagcacc tggccgtaga cgcacatggt
     5761 gtccttggtg gtgaaggcgt cgagcgagcc gcccaccttc tcgatgtcgg tcgagatctg
     5821 cttcgcgctg cgccggttcg tacccttgaa gagcatgtgc tccatgaaat ggcacagccc
     5881 cacctcgcgc ggggattcgt cgcgcgcgcc cgtgcgggtc caaacgccga tgcagaaggc
     5941 cttcgagatg gggttcttct cgtgcaggat cgtgagcccg ctgggcaaca cgatcttgtt
     6001 ggcaacgcgt gcgggtgtca ttcttcctcc catcatcgtc cacaaaacga ctgcgcccgc
     6061 ccacaagatg gtacctgtga gcgggccgcg caatacgacc gaagtctccg atatcgccgc
     6121 tagcgccgcg agctgtgacg gcgtccgccg ccgccgcggc cgtggtcgcg gtcgctgcgt
     6181 tcgcgatgcg gacgctcgct gcgctcttcc tcctggtagc cttccggctt ctcgaggagg
     6241 accttgcggc tcaagcgaac cttgccgtcg ggctggactt cgagcacctt gaccttgacg
     6301 atctcgccca tctgcagctc gtcttcgacg cgctccagac ggcggtgcgc gatctccgag
     6361 atgtgcaaga gcccgtcgcg tcccggcatg atctgcacga acgcgccgaa gggcatgatg
     6421 ctgcgcactt cgccttcgta caccttgccg acttcgggct cggcggtgat ctccttgatg
     6481 cggcccagcg cgatctccgc gctctccgta ttgacggcga tcacgcgcac ggttccgtcg
     6541 tcctcgatct cgatcttggc gcccgactcc tcctggatgc gcttgacgac cttgcctccc
     6601 gggccgatga tctcgcggat cttgtcgacc gggatgtgga tcgtggtcac cttgggagcg
     6661 aacggcgaga tgtccgcgcg cgcctgcgcg atggccttgt tcatctcctg caggatgtgc
     6721 atgcggcccg tcttggcctg cgcgagcgcc ttcatcatga cggcgtcgga gataccggcg
     6781 atcttggtgt cgagctggaa ggcagtgatg ccttccgcgg tgcccgtcac cttgaagtcc
     6841 atatcgccca ggtgatcctc ggcgcccagg atgtcgctca acacggccac gcggtcgtcc
     6901 tcgattacga ggcccatcgc gatgcccgcg accggcttct tgatcgggat gccgccgtcc
     6961 atcagcgcca ggctcccgcc gcacaccgac gccatcgagc tcgagccgtt ggactcgaga
     7021 atctccgaga cgattcggat cgtgtacggg aactcgtcct tgggcgggat catggggcgc
     7081 agcgcgcgct cggcgagggc gccgtgtccg atctcgcgcc taccgggccc gccgaagcgg
     7141 cccacttcgc cgacactgaa ggccgggaag ttgtagtgga gcatgtagcg cttccagctc
     7201 tcgccttcga tggtgtcgag catctgctcg tcgtcggtgg tgccgagcgt ggtcgtcacc
     7261 aggctctggg tctcgccgcg cgtgaacagc gcggagccgt gtgcgcgcgg gagcacgccc
     7321 acttcgcagg tgatggggcg aatgtcggtg ctgccgcgtc cgtcgaggcg cttgcccttg
     7381 tcgaggatca tgccgcgcat gagtttcttc tcgatctcct cgagcaccag cgccacgtag
     7441 gcgcccttct cgggatagtc gacctcgtac ttagcgagcg cgtccttctg aatggtcttg
     7501 agcgcgtcct ggcggtcgag cttcttcgcg atgaagagcg cttgaccgat gcggtcggag
     7561 aactccttgg cgatcttggc gtccagctcg gccagtcgct cgggaacctc gaccacgcgc
     7621 ttcttcttgc cgccgaacaa cgacaccagc tcgcgctgga gcgcgttgag gcgcttgatc
     7681 tcctcgtggc cgaagcgcac cgcggccagc atgtcctctt cggacacctc ggaacaaccg
     7741 ccctcgacca tcatcacgcc ggtgtcggtg ccggcgatga tgacgtccag atcgctggcc
     7801 tcgttctgct cggtggtcgg gttgacgatg aactgcccgt cgatgcgacc gacgcgcacg
     7861 cccgagacga tgtcttggaa gggaatgtcg gacagggcca gcgcggccga cgcgcccacg
     7921 atgctgatca cgtcgggcgc gttcacgccg tcgtagctga taggggtcgc gatgacctgc
     7981 gtctcgcaca tgaagtgctt gtcgaagagc ggccggatgg gccggtcgat cagtcgcgag
     8041 gtgagggttt cgcgctcgcc ggggcggcct tcgcgcttga agaagccccc gggaatgcgg
     8101 ccggaagcgt agaacttctc gcggtactcg accgtgagcg gaaagaagtc gcggtcggtc
     8161 ttttgctggg cggccacgac ggccaccagg accatggtgt cacccatgcg cacgacgacg
     8221 gatccgtcgg cctgcttggc catgcgtccg gtttcgatac tgaacggctg gccgttgacg
     8281 attcgtttca ctgtatgact tgccatattg attctcggac ctatccaagg tctcactggt
     8341 aaagcgcgcg tacacacgcg cgcccgagag agaagccgat caacccgcgg agtcgttccc
     8401 ggaggaacta cttccgcaag ttgagctgct tgatcatctc ccggtagcta tcgattttct
     8461 ggttcttcaa gtaatccagg agttttctcc ggcgaccgac caacttcaac aggccgcgac
     8521 gggacgcatg gtctttcttg tgaacctgga agtgctcggt caacatgcgg atgcgctcgg
     8581 tgaggagagc aatctgaact tctgccgacc cggagtcttt gtcgtggagc ttgtacttcg
     8641 caatgatgtc actcttctgc tgcggagtca ttaccatcgt ctgatcacct cctgataatc
     8701 ggtacgcgac acgatggtta cagccagcga aaggccagcc gttcgacttg gcgggaaccc
     8761 taccacaggg ccccgggggt gtaaagtgcc aaatgacgcg gcctagcccc tcgccaggcg
     8821 ctcccgcgcg gcccggcggt cctcattgag ctgctcgatc agcgcggtca cggtcgggaa
     8881 gcgcgcctcg gcgcgcaggt aggcctcgca gtggaccgat agctcgtcgc cgtagatgtg
     8941 gtggtcgaag tcgaagacgt ggacctccat ctcgtcgtga tcgacgcgct tgatggtggg
     9001 cgagcgcccg atgttcatca tgccgtcgta gacccggccg cgccacccca cccgaaccgc
     9061 gtacacgccc gacgacggcc actgcttgtg cgggtgcgtc accgcgacgt tggcggtcgg
     9121 gaaaccgagg tcccggcccc ggccctgtcc ggtggcgacc gtgccccgca cgagatacgc
     9181 gtgccccatc aggtgattcg cgcgctcgag gtcggcggct tgcagggccc gtctaatatt
     9241 ggtgctcgat atcttctcac cgtcgacgta cacgggcggg atcacggtga cggcgaagcc
     9301 gcgtcgcacg ccctcttccg ccacgcgttc ggggctcccc tcgcgctggt ggccgaagtg
     9361 gcagtcatag cccatcacca cttcgcgcat gtccatcgcg tccatgaagt agcgctggat
     9421 gaagtcgcgg tactcgatgc gcgcggtggc ttcgtcgaag gggagcacga acacgccgtc
     9481 gaggccgaac ttcgacagca gctcgatgcg ctccgcaacg gtggtgagaa tcggtggggc
     9541 ctcgcgcgag tgcgtgacca ccagcgggtg cgggtcgaac gtgatcgccc acgcggaggc
     9601 cacgcggccg ctctcgcggc gtgagaccaa ttcttcgagt atcttgcggt ggccgaggtg
     9661 gacgccgtcg aaaacaccga tggtcacggc cgagtcgcgc ggggcgagcg cgcgcagctc
     9721 ttcgagactt cgggcgactg cgagcggccg catcgtttca gcgcgagacg cgcgccgcac
     9781 cggcgcccgt gccgcccggg ctcgcttcga cgaagagacg gaaggaatcg cacacgcgca
     9841 agggatcgcg cgggtggtct ccgccgcgcg tcccgatggc gaggagtgtt ccctcttcgt
     9901 ccaggatgcg aagcggcccc gcgatggcgc cgatggtagg cgcccccacg gtctccacca
     9961 cgtcgttgcg caagggtgcc gcaccgaaca agagcgcgcg cttggcgcgc gtcgacacga
    10021 cgacgccggg aagaaacgac agggcttccc cgaggtcgag accaattact tcgtcggccc
    10081 gctctggcac cagacactcg gacgagaccg cgtcttcgac gcggtaattg ccgatgcgcg
    10141 agcgcgcgag gctcgcgatc gacgcgggca gccccagctt cgcccccagg tcgcgcgcga
    10201 gcgcgcgcac gtaggtgccg cgcgagcagc gcacgacgca gtgcacgatc ggcagttcca
    10261 ccgagacgac gtcgaaggcg tggacgcgca ccgtgcgcgg cgaggcctcg gccggctcgc
    10321 ccgcgcgcgc gaggtcgctg aagcggcgcc cacccagctt gagcgcggaa aacgtgggcg
    10381 gcaccatgac gtaatcgccc acgaactggg ccgccgccgc gcgcatctct tcgatcgtga
    10441 ggggcggaac cggcgcctcg cggaccacgt cgccttcggc gtcgagggtc gtggtctcca
    10501 cacccaggcg cacgtcgaag cggtactcct tctcgaggtt catgaagtgc tccaccgcgc
    10561 gcgtcgcgtg gcccgtgcac acgagcagca cgccgcgcgc gagcgcgtcg agggtgccgg
    10621 cgtggcccac gcgccgcagt cccgagacgc gccgcagcgc ggccacgatc tcgaacgagg
    10681 tgggcccggg cactttgtcg atcacgaaca ctcggttgtc gaagcgattg gtcattcgtc
    10741 cttcggttcc ggctcgtcat tcgggatggt gccggtctcg cgcagcgcct gctcgatgcg
    10801 gaacgcgcgg tccagcgagt cgtcgtatac ccagcgcaat tcgggtgcta cccgcacgtc
    10861 catgtggcgc acgagcacgc ggcgcaggaa acccgacgcg gccttcagcg tcttgagagt
    10921 catcgcgcgc tgcgcctcgt cccccatcac gctgaacagc accttggcgc tcgagaggtc
    10981 gttggtcacg cgcacgccgg tgatggtgat cagccccacg cgcgggtcct tgatctcggt
    11041 caataccaac tcgctcaaga cttccttgat gagctcgttg acgcgctcga ttctgtaatt
    11101 cggtctggtc atctatcgaa attcctcgag ggcatcggcc agctccacgc ggtggtcgcc
    11161 ttcgatgaag cgtcgcactt gcccgcaggt cccgtcgacc gactcccgat ccgagccgac
    11221 gatcgccacc gcgatcaggc agcgctgcca cttgtcctga taatcgatct ccgccaccga
    11281 cacgttgaag cggctgcgga gcctggcttt gaggctcttc agcacgaagc gcttctcttt
    11341 caacgaagaa gagccgggga tcagaaggtc gacggtcaag agcttgacca acatcgcacg
    11401 aaccgcgccc ggcaggtgac tggccgcggc gccgttagaa tacgcgggcc ttttcttcga
    11461 cgacgaacgc ctcgatgatg tcgcgctcgg tgaaatcgtc gaagccggag accccgatac
    11521 cgcactcgaa gttctgcgcc acctcgcgcg cgtcgtcctg gaagcgcttg agcgagccga
    11581 tcttgccctt ggcgattact tcgccgttgc gaatgacgcg cgcgctggcg ttgcgctgga
    11641 tcgtcccctc caccaccatg cagcccgcga tggtaccgag cttcgacacc cggaagatct
    11701 tgcgcacctc ggcgcgtccg agcacgcgct cgacgaacac cggttccagc atccccttga
    11761 gcgccttgtc gacgtcttcg atggcctcgt aaatgatctt gtagctgcgg atctcgatgc
    11821 ccttgagctt cgcgatgtcc ttggccttgg gcgttacctt ggtattgaac gcgatgatca
    11881 cggcgttggt gctctgcgcc agcagcacgt ccgactccga gacgccaccg acgccaccgt
    11941 gcacgaggtt gattttgatc ccctcgatgt tcagcggcag gaggctgtcg cgcagcgctt
    12001 cgagcgagcc gttggtgtcg gctttcaaga ccacgttcaa ttccttcgcg gccccgccct
    12061 gaatttgggc atagagctct tccaaggtga gggccttggt gggctccagg cccttcgcgc
    12121 gcaggttctc ctggcgcttc tgcgccactt cgcgcgcctc gcgctcgttc ttgaccgcga
    12181 tgaactggtc gccggcctcg ggcatgccgt cggcgccgag cacgaccacc ggggtcgacg
    12241 gacccgcctc gcgcagcgcg cgacccgcgt ggtcgaacag cgagcgcacc ttgccgtact
    12301 cgttgcccac cacgaacacg tcgccgatgc gaagcgtgcc ttgctgcacg agcacggtga
    12361 tcaggattcc gcgcccctct tccttgcgca cttcgaccac gacgccctgt gcgcgcgcgg
    12421 tcgggtcggc cttgagctcc gcgagctcgg cctgcaagag gatcatctcg agcagcttat
    12481 cgatgttctt gccggtcttg gccgagactt ccaccgagac cacgtcgccg ccgtagcctt
    12541 cgaccaggac gctctgctgt gccagctgct gcatcaaccc ggccgggttc gcggtcggca
    12601 aatcgacctt gttcatggcc acgatcatcg gcaccttggc cgcgcgcacg tggttgatcg
    12661 cctcgatggt ctgcggcatg acgccgtcgt ccgcggccac aaccagcacg acgatgtcgg
    12721 tgctcttcgc gccgcgcgca cgcatggcgg tgaaggcctc gtggcccgga gtgtcgatga
    12781 aggtgatctt cccgttgggt gtcgtgactt cgtacgcgcc gacgtgctgc gtgatgccgc
    12841 ccgcttcgcc ggcggcgacg ttggccttgc gaatgtaatc gaggatcgag gtcttgccgt
    12901 ggtcgacgtg acccatgacg gtcaccaccg gcgcgcgcgc caccagcttg gtcggatcga
    12961 cctgggcgtc gagtttgagc tgggtctcgc cgaacgacgc ttccacgtcg acgcggcggc
    13021 ccaattcttc cgccaccaac tcgatggtgt cgcggtcgag tgcggcgctg gcatcggcct
    13081 ggaggtcgag ctgcgacatg cgcgagagaa cgtcgtcggc cgaaacgccg agggcgtatc
    13141 cgaggtcggc gggtgtcgca ccctcctgca cgcgcacggg agggagatcg accggcgcca
    13201 ccgtggcggc gcgcgtcttg cgccgcttgg tcttgcgcgt ggtctccatc ttggccagcg
    13261 tgcgccgcac gctctcgcgc acctgctcgg catcgctggt cttggcgtgc acgtgaccgt
    13321 gacgcttgtg ctcttcggtc gcctgcaccc actcctcgac ctcgtcggcc ggagtgtcgg
    13381 gctcgatggc gatgagcggc tccacggccg ccttcgccaa ctcgtcgagc ggtgtcagct
    13441 tcttgggctt gggcggggcc ttgggcgcgg gcgccacctt cagcgcgggc ttctccgctt
    13501 cggcctccgc gcgcttgctc gtcttggccg cggcgggagc ggtcgcgacc gacgacgact
    13561 tcttggccga cttggcttcc ttgacctccg ccttggcctc cgctttcggc tcgaccgatg
    13621 gcgcgggctc ggcggccggc ttggccgcct tcgcgagttt gaccttgggc ttctccttgg
    13681 cgggcgcttc gaccaccggc tcgggctcgg ggcccttcgc cttgcgcagc ttgaccttcg
    13741 ctttggcggt atccgcgctc gcggcggcgg cggccttgga cgccttcgac gtcgttttgc
    13801 ccttgccctt cttggccgac gcgcccgagc ggctgaagtg gcgatcgagt gcggtcagca
    13861 tgtcgtagtc gacggcgctg ccggcgctcg ccacctggta gcccgcgttc ttcaacaacg
    13921 cgacgagctc gtcgttggtg acgttatact tcttggccaa ctcggagatg cgcatttagt
    13981 ccttcgtgtc ctctgcttcg ggattcgatt cggcgtcgcc gtcgccggtc gcttcggcgg
    14041 tcggctccgc ggaaatctcc gccggagtct cggcggtagt ctccgccggt gcttcggcgg
    14101 gctcggttga cgccggctcc gcggccgctt ccgcctcgac ctcggccggg tcgcggaaca
    14161 actgcgcggc cgtgagcttc ttggtttcct tcttgggctc ttcggtctcc gcttcgagca
    14221 tgctttcgtc gaagagcggc ttctcttgcc gcttcttctc ctcgcggtcc tcgttctcct
    14281 tgcggatcaa ttcttcgagc gccttctcga gctccttgac ggtgccctgc gcggtctcca
    14341 tcagcctctc gaccgtgcgc tcgccgaaac ccgggatcgt gagcaacgac ccgaccggag
    14401 tcttgatcag cttctgcacg gtgttgatgc cggcctcgcg cagcttctcc gccatcttgt
    14461 cggaaacacc caccatctct tcgatcggca tgagcaggtg ttcctgcagg cgctggcgct
    14521 gctcgagctc gcgcgacgag accaattcga tctgccagcc ggtcaagcgc gacgcgagtc
    14581 gcacattctg gccttccttg ccgatcgcga gcgagagctg gtcttcttcg accaccacgg
    14641 taaccgtctt ggtttcgatg tcggggcgaa cctgggtgat cttggccggc gagagcgcgc
    14701 gcgagacgaa ggtctgcacg tcgtcgctcc acggcacgat gtcgatgcgt tcgcccgaaa
    14761 gctcgttgac gacggcttgc acgcgcgagc ccttcatgcc cacgcacgcg cccaccgcgt
    14821 ccacgcggtc gtcgcgcgag tagacggcga tcttcgagcg cccgcccgct tcgcgcgcga
    14881 tgctcttgat ctcgatcagg ccttcgtaga tttcggggac ttcctggatg aacagggcct
    14941 tcaggaactc gggactggtg cgcgagagcg tgatcatcgg ccccttggct tccttgtcga
    15001 ccttcatcac gacggcgcgg atcgacgagc cctgctggta gcgctcgcgg cgaatctggt
    15061 ccttcttggg catgaacgcc tcggcgctgc ccaggttgac caggatgccg gtgcggtcga
    15121 tctgctggac ggagccggag acgatctcgt tgacgcggtc cttgtactct tcgaacacgc
    15181 ggtcgcgctc gaactcgcga atcttctgca tcaggatctg cttggtgacc tgaatcgcgt
    15241 tgcggccgaa ctcctcgaac gggagattga tctcgatggc gtcgccggct tcggcgccct
    15301 cggcgtattc ctgcgcgtcg accaccgaaa tctgaatgct ggggtcctcg accgcgtcga
    15361 caacgttctt gaccaggaac agctcgatcg acccgtggcc gtcgtcctcg atgcgcactt
    15421 ccgcgttcgg cccgtacttc ctgcgcacgg ccgacgcgaa gccctgcgtc agggtgtcca
    15481 tgaggatggc gcgatccagc cgccgctcgc ggatgagctg gttgaacgcc tctgcaaagt
    15541 ggatgctcat ttactcctcg cctccgcgcc cttcccaatt gggggggcgc cggtgcttct
    15601 ctctcttctt gtcgatcttg tattcctgat tgattagatt cgctcgcacg acctcgcgca
    15661 gccccaccgc cacttcgccc gcgtcggtat ccagggtgag cgcgccgttc acgcaggaac
    15721 gaatgcggcc aatatgcgtc gcccgcgcgc ccgcgcgctc gacgaccacc ttcgcttcgt
    15781 gtcccacgaa gcgctgaaaa tgcgcctcgg tcacgagcgg ccggttgctt cccggcgacg
    15841 aaacctcgag cagaaaacta ccggggaacg gatcttcccg atcgaacacg agcccgaggg
    15901 tgcgactgac gagcgcgcac tcgccgacgg tgactccgcc gggcttgtcg atgaacaacc
    15961 gcacgacggg gtgtcgcggg ccaccgacca cctcgagctt cacgcactcg aagccgagcg
    16021 tctccagctc gggcacgatc agcttctcga gttcctgttc tgtcacgatc agcccggcga
    16081 cggatggata cgaaccggac ggcaataaaa aagtgggcga ccccgcccac tggtcagcac
    16141 acccacaaca cacggaattt ctagcacatc gctcgggaaa gcgcaagccc ctcggcgaca
    16201 cgtgcacgag ccgcgggtgg cgtccggcgg gcgcccacaa acgagggcct actagtgcgc
    16261 atgcgcggta ggcccgagag cagtgggctg ccggacgaca ggagccgcgg cgggtacctg
    16321 cccggccgcg tcactcggcc ggaaagggcc tgtcgtagag cagatctcga agcaggacaa
    16381 gcacttggga gagcttggcg gggtcgacct gggacaggtc gtcgccgagg gtgtgccagg
    16441 cggcgtagtc gtagccgaag aggtcgatgg cggggatgcc ggcctggagc agggggacgt
    16501 ggtcgtcgat catcggggcg ccgtcggcgg cttcgaaata gtcgaggcgg agagccgccg
    16561 cgcgggcgaa gacgtggtcc aggaaggcgc gcgcgtgttc cttggagaag ggctcgcgac
    16621 ggatgcgtgt ccccttcccg cccaccatgt cgagcaggat ggcgccctgc gggcggtacc
    16681 cgttgaggtt catgacgaag tgcttcgagc ccaagaggta gtggtcgatg ttcccccgct
    16741 tgccgtagtc ctcgccgtcg aacagcacca cgtcgacgcc gatctcgccc gggctcgcga
    16801 gtccgaccag gcgcgcgacc tccagcagga cggccacacc cgtggccccg tcgaccgcgc
    16861 ccgggatggg ctcggtccac aaggtcgagt cgcgctcttg atcggcccac ggacgcgagt
    16921 cgtagtgcgc gcacagcatg atgcggcgcg tgcgctccgg cgcgaagctc ccgacgacgt
    16981 tgatcaatcg gagccggcgc aactcgtacg gatcctcgac ctcgaaggac tgcagcgaca
    17041 cgcgcgcgcc gtagcgctcg agggcgcgcg cgaggtagcg cgcggccgcg tcgcgcgcgg
    17101 gagtgccggg cacgcgcggg ccgaaggaga gctggcgctc gacatggacc atgacgctgt
    17161 cgaccgagcc cgccgggggc tcgctcgcgc cgcagccggc cgccatcgcg gccagggccg
    17221 ccgcactcgc gagcgtagcg gcgcgcgcac ggcgcaagag agcttctcca cgcggcatcg
    17281 ttttcagaag ttga
//
LOCUS       DASPGK010000170         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1683879, whole genome shotgun sequence.
ACCESSION   DASPGK010000170 DASPGK010000000
VERSION     DASPGK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683879"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="VEC56_04925"
     CDS             complement(<1..1721)
                     /locus_tag="VEC56_04925"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HXV13528.1"
                     /translation="MRPPLLDRRSSHQPRSTIATTIAFGLAASLGAAIATPLNGAPDE
                     ADPRSVEWVCAHAPERVSLLFDSLNLDRPALSKVKQAVLASDYPAACHALLQYYRTAP
                     SAKWLRHPVVKESRKDDARADAILSDELEFYGERATVPRTAAGGLDWTYAGPRNDPEW
                     NWSLNWMGYLGPLTAGYFKTGRRDYVERIDQDVRDWILANPRPSAATRAGGWRGLEVA
                     TRVGNWKSVFYGLQAVDEFSPAARILMLSSLVEHAQHLMLFHRRDANNWTVSEMNALG
                     TVGCAWPEFRDAGGWRAYSQENIGRLAGELVYPDGAEAELTSGYHRIATETFDGYVDT
                     FRRFGYPVADSIAVDIKRMWTYLAYTLRPDGTSPQNNDSDRSDIREKLTAAAMTYDRP
                     DWSYIASNGREGNRPRIGPSVTFPWAGHAVMRNGWGADAHWAFFDAGPFGVAHQHRDK
                     LHLSIDAFGRPLLVDTGRFTYSEGPQRDYFAGSAGHNVLLIDGAGQNKTPERAQRPMP
                     GGDYGSTAELDFARGIFDAGFSGTKGRAVHTRTVVYIKDRFWIVADRVETDRSRTVEA
                     LWHFAPD"
     gene            complement(1737..>2287)
                     /locus_tag="VEC56_04930"
     CDS             complement(1737..>2287)
                     /locus_tag="VEC56_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXV13529.1"
                     /translation="ETFALPNPFFTIATQNPIEQEGTYPLPEAQLDRFMFNIRVDYPS
                     EEEEIQIVETTTSPQATTPKVVLKASDILRTQELVRTVPVDSHVLRYAIRLVRATRDD
                     SIPQVKQYVSWGAGPRAGQNLVLGAKVRAVMNGRPIPSIDDVRAIAHPVMRHRIVTNF
                     NADADGVSTDVLIDRLLESVPA"
BASE COUNT          351 a          742 c          802 g          392 t
ORIGIN      
        1 cagtcgggcg cgaagtgcca gagcgcttcg accgtgcgcg agcggtcggt ctcgacgcga
       61 tcggcgacga tccagaagcg gtcctttata tagacgacgg tgcgtgtgtg caccgcgcgc
      121 cccttggtgc cggagaagcc cgcgtcgaag atgccgcgcg cgaaatcgag ctccgcggtc
      181 gatccgtagt cgccgccggg catggggcgc tgcgcgcgct cgggcgtctt gttctgcccc
      241 gcgccgtcga tgaggagcac gttgtgcccc gccgaccccg cgaagtagtc gcgctgcggt
      301 ccctccgaat acgtgaagcg ccccgtgtcg accaggagcg gccgcccgaa tgcgtcgatc
      361 gagaggtgaa gtttgtcgcg gtgctgatgc gcgacgccga acgggccggc gtcgaagaag
      421 gcccagtgcg cgtcggcgcc ccacccgttt cgcatgaccg cgtgtcccgc ccacgggaac
      481 gtgacggatg gaccgatgcg cggccggttg ccctcgcgcc cgttgctcgc gatataactc
      541 cagtccgggc gatcgtaggt catcgcggcg gccgtcaatt tctcgcggat atcgctgcgg
      601 tcggagtcgt tgttctgcgg cgaggtcccg tcgggtcgca gcgtgtacgc gagataggtc
      661 cacatgcgct tgatgtcgac cgcgatcgag tccgccaccg gatagccgaa acggcggaac
      721 gtgtcgacgt agccatcgaa cgtctcggtg gcgatgcggt ggtacccact cgtgagctcc
      781 gcctccgcgc cgtccgggta cacgagctcg cccgcgagcc ggccgatgtt ctcctgcgag
      841 tacgcgcgcc agccgcccgc gtcgcgaaac tcgggccacg cgcatcctac cgtccccagc
      901 gcgttcatct cgctcaccgt ccagttgttg gcgtcacgcc ggtgaaacag catcaggtgc
      961 tgcgcgtgct cgaccaggct cgacagcatc aggatgcgtg cggccggcga gaactcgtcg
     1021 accgcctgca gtccgtagaa caccgacttc cagttgccga cccgcgtggc gacttcgagc
     1081 ccgcgccacc cgcccgcgcg cgtcgcggcg gacggacgcg ggttggcgag aatccagtcg
     1141 cgcacgtctt ggtcgatccg ttcgacgtaa tcgcggcgcc cggtcttgaa atagccggcc
     1201 gtgagcgggc ccaagtagcc catccaattg agcgaccagt tccactccgg gtcgttgcgc
     1261 ggaccggcgt aggtccaatc gaggccgccc gccgcggtgc gcggcacggt ggcgcgttca
     1321 ccgtagaatt cgagctcgtc ggagagaatg gcgtccgctc gcgcatcgtc cttgcgcgat
     1381 tccttcacca ccggatgacg gagccacttc gcgctggggg cggttcgata gtattgaagg
     1441 agcgcgtggc acgcggccgg gtagtcgctc gcgagcacgg cttgcttgac cttggagagc
     1501 gcggggcggt cgagattgag actgtcgaag aggaggctca cgcgctccgg cgcgtgcgcg
     1561 cacacccact cgacgctgcg cggatcggct tcatcgggcg cgccgttgag gggcgtcgcg
     1621 atcgcggcgc cgagcgaagc ggccaggccg aatgcgatgg tggtcgcgat cgtacttcgc
     1681 ggctggtgcg acgagcgccg gtcgaggagg ggcggccgca tgtgccgatg gagacgctac
     1741 gcgggcacgc tctcgagcag ccggtcgatc aacacgtcgg tcgagacgcc gtccgcgtcg
     1801 gcgttgaagt tggtgacgat gcggtggcgc atcaccggat gcgcgattgc gcgcacgtcg
     1861 tcgatgctcg gaatggggcg gccgttcatg acggcgcgca ctttggcgcc gagcaccaag
     1921 ttctgtcccg cgcgcgggcc ggcgccccac gacacgtact gcttcacctg cggaatcgag
     1981 tcgtcgcgcg tcgcgcgcac caatcgaatc gcgtagcgca gtacgtgcga gtcgaccgga
     2041 accgtccgca ccagctcctg cgtgcgaaga atgtcgctcg ccttgagcac gaccttgggg
     2101 gtggtggcct gcggcgacgt ggtggtttcg acgatctgga tctcttcctc ttcggacgga
     2161 tagtcgacgc gaatgttgaa catgaagcgg tcgagctggg cttcggggag cggataggtt
     2221 ccctcctgct cgatcggatt ctgcgtggcg atggtaaaga acggattggg caacgcaaac
     2281 gtctctc
//
LOCUS       DASPGK010000171         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1215744, whole genome shotgun sequence.
ACCESSION   DASPGK010000171 DASPGK010000000
VERSION     DASPGK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215744"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VEC56_04935"
     CDS             complement(<1..1029)
                     /locus_tag="VEC56_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXV13530.1"
                     /translation="MASRNKKTWREKVYDPAVKKTPERKRRFRTSSGIEIEPLYTSPG
                     DLDVGSEEKVTFPGEYPFTRGVYPSMYRSRPWTTRQYAGFGTARETNQRFHSLLKRGQ
                     NGLSVAFDLPTQMGLDSDNPLALGEVGRVGVAIDTVDDLEVLFDRIPLESVSTSMTIN
                     ATASVLLAMYAVVHEERGGKRRDLSGTVQNDILKEYIARGTYIYPPAPSMRVITDLFA
                     YAKEEWPEWNTISISGYHIREAGSTAPQELAFTLADGIAYVEAAIRAGLDADAFASQL
                     SFFFNVHNDFLEEIAKFRAARRMWAKIMRERFRAKNPRSWLLRFHAQTAGSTLAAQQP
                     DVNIVRVAI"
     gene            complement(1029..1547)
                     /locus_tag="VEC56_04940"
     CDS             complement(1029..1547)
                     /locus_tag="VEC56_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXV13531.1"
                     /translation="MKFYVRVDGTERVLHVSRDGAVYRVDVDGRVLNVDARNFGDKDA
                     FSLLIDRKSYLVEAAPVKPERGVYFARVLGRHYDVEVLDELLVAVRDAERAREHTGAF
                     TLRAPMPGLVVQVRVTPGARVSVGEAVVVMEAMKMRNELASEVAGVVTSVAVAEGDKV
                     DSQTPLVTIERE"
     gene            complement(1544..3052)
                     /gene="accC"
                     /locus_tag="VEC56_04945"
     CDS             complement(1544..3052)
                     /gene="accC"
                     /locus_tag="VEC56_04945"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXV13532.1"
                     /translation="MFAKILIANRGEIAVRVARACKEMGITPVAIYSDADRTALHVAH
                     AYEAYRVGGPESRESYLNIPNILAACRESGAEAVHPGYGFLAENADFAQACADAGVAF
                     IGPSANAIRAMGNKLAARAAMKKAGVPTVPGSDVPVTDAREAARLAREIGYPVLLKAA
                     AGGGGKGMRVVRTDAEFEAALKLTTGEAKAAFGDASVYIEKFIERPKHIEIQVMADAH
                     GNVVTYGERECSMQRRYQKVIEEAPSPIVTPQMRASLSDAARKAAIAVGYTGAGTVEF
                     IVDSERRFFFLEMNTRLQVEHPVTEMVYGVDLVKQQIEVAGGAALTVQQSDVVPRGHA
                     IEMRVYAEDPAHGFLPSIGTIKRLVLPQGPGVRNENGVYEGYEIPIYYDPMLGKLVVY
                     ADTREDAIQRARRALREYRCEPVRTNVDFLLWALNEPAFVDGSYDTKHIEAHFDPSQL
                     HQRDEEVELAAIAAAIAAFDYRSTVRYATLDESKDGAWRRAARADATKRWSR"
     gene            complement(3070..>3662)
                     /locus_tag="VEC56_04950"
     CDS             complement(3070..>3662)
                     /locus_tag="VEC56_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXV13533.1"
                     /translation="IGVVANQPKSLAGVLDIKSSIKAARFVRFCDAFNIPLVTFEDVP
                     GFLPGTEQEWGGIIKHGAKLLFAYCEATVPKITVITRKAYGGAYDVMSSKHIRADYNV
                     AWPSAELAVMGAEGAVNILNKREIESAKDPAAERARRVQEYNEKFANPYIAASLGYID
                     DVIEPADTRPKIIAALESLVNKRQSMPPKKHGNIPL"
BASE COUNT          568 a         1200 c         1229 g          665 t
ORIGIN      
        1 gatcgcgacg cgcacgatgt tgacgtccgg ctgctgcgcc gcgagcgtcg agcccgcggt
       61 ctgcgcgtgg aagcggagca gccacgagcg cgggttcttc gcgcggaacc gctcgcgcat
      121 gatcttcgcc cacatgcgcc gcgccgcgcg gaacttggcg atctcctcga ggaagtcgtt
      181 gtgcacgttg aagaagaacg agagctggga tgcgaacgcg tcggcgtcga gcccggcgcg
      241 gatcgccgcc tcgacgtagg cgatcccgtc ggcgagcgtg aacgcgagct cctgcggcgc
      301 ggtcgagccc gcctcgcgga tgtgatagcc gctgatcgag atcgtgttcc actccggcca
      361 ctcctctttc gcgtaggcga agaggtcggt gatgacgcgc atcgaaggcg cgggcggata
      421 gatgtacgtg ccgcgcgcga tgtactcctt caagatgtcg ttctgcacgg tgccggagag
      481 atcgcggcgc ttgcccccgc gttcctcgtg cacgacggcg tacatggcca agaggaccga
      541 cgcggtcgcg ttgatggtca tcgacgtgct gacggactcg agtggaatcc gatcgaagag
      601 cacctccaga tcgtccacgg tgtcgatggc gacgccgacg cggccgactt cgcccagggc
      661 cagcgggttg tcggagtcga ggcccatctg ggtggggagg tcgaacgcga ccgagagtcc
      721 gttctgcccg cgcttcaata gagaatgaaa gcgctggttg gtctcgcgcg cagtcccgaa
      781 gccggcgtac tggcgcgtgg tccacggccg gctgcggtac atcgacggat acacgccgcg
      841 tgtgaatggg tattccccgg ggaaggtgac tttctcctcg ctgccgacgt caaggtcccc
      901 gggagatgtg tacagcggct caatctcgat tcccgaggaa gtgcgaaaac gtcgtttccg
      961 ctccggcgtc tttttcaccg ccgggtcgta gaccttctcg cgccacgtct tcttgttcct
     1021 cgacgccatc actctctctc gatcgtcacc agcggggtct gcgagtcgac cttgtcgccc
     1081 tcggccacgg cgaccgacgt caccacaccg gccacttcgc tcgcgagttc gttgcgcatc
     1141 ttcatcgctt ccatgacgac caccgcttca cccacggaaa cgcgcgcccc gggtgttacg
     1201 cgcacttgga ccacgagccc cggcatgggc gcgcgcagag tgaaggcgcc cgtgtgctcg
     1261 cgcgctctct cggcgtcgcg taccgcgacc agcaattcgt ccagcacctc gacgtcgtag
     1321 tggcggccca gcacgcgcgc gaaatacacg ccgcgctcgg gtttcacggg ggcggcttcg
     1381 actaggtagg acttgcggtc gatcaagagc gagaaggcgt ccttgtcgcc gaagttgcgc
     1441 gcatccacgt tgagcacgcg cccgtcgacg tcgacgcgat agaccgcgcc gtcgcgggag
     1501 acgtgcagca cccgctcggt tccgtcgacg cggacgtaga acttcatcgc gaccaccgct
     1561 tggtggcgtc ggcgcgcgcg gcgcgacgcc aggcgccgtc cttggactcg tcgagggtgg
     1621 cgtaacgtac ggtgctgcgg tagtcgaaag cggcgatggc ggcggcgatc gcggccagct
     1681 cgacttcctc gtcgcgctgg tgcagttgcg acggatcgaa gtgcgcctcg atgtgcttgg
     1741 tgtcgtagct cccgtcgacg aaggcaggct cattcagcgc ccacaatagg aagtcgacgt
     1801 tggtgcgcac cggctcgcag cggtactcgc gcagcgcgcg tcgcgcgcgc tggatcgcat
     1861 cctcgcgcgt gtcggcgtac accaccagct tgccgagcat gggatcgtaa taaatcggaa
     1921 tctcgtagcc ctcgtacacg ccgttctcgt tgcgcacgcc ggggccctgg gggagcacca
     1981 agcgcttgat ggtgccgatc gaggggagga agccgtgcgc ggggtcttcg gcgtaaacac
     2041 gcatctcgat cgcgtgcccg cgcggcacga catcgctctg ctggaccgtg agcgccgcgc
     2101 cgccggccac ttcgatctgc tgcttgacca agtcgacgcc gtagaccatc tcggtcaccg
     2161 ggtgctcgac ctgcaggcgc gtgttcatct cgaggaagaa gaagcgccgt tcggaatcga
     2221 cgatgaactc gaccgtgccc gcgccggtgt agcccaccgc aatcgcggcc ttgcgcgcgg
     2281 cgtcggagag ggaggcgcgc atctgtggcg tcacgatggg cgaaggcgcc tcttcgatca
     2341 ccttctggta gcgacgctgc atcgaacact cgcgctcgcc gtacgtgacc acattgccgt
     2401 gcgcgtcggc catgacttgg atctcgatgt gcttgggccg ctcgatgaac ttctcgatat
     2461 aaaccgatgc gtccccgaag gcggccttgg cttcgcccgt ggtgagcttg agcgcggctt
     2521 cgaactcggc gtcggtgcga acgactcgca tccccttgcc cccgcctccg gcggcggcct
     2581 tgagcaagac cgggtaaccg atctcgcgcg ccagacgcgc ggcttcgcgc gcgtccgtca
     2641 cgggtacgtc gctgccgggg acggtaggaa cgccggcctt cttcatcgcg gcacgcgccg
     2701 cgagcttgtt gcccatggcg cgaatcgcgt tcgcggaggg accaatgaac gcgacgcccg
     2761 cgtcggcgca cgcctgcgcg aagtcggcgt tttcggccaa gaagccgtag ccgggatgga
     2821 ccgcctccgc gccgctctcg cggcacgcgg ccaggatgtt gggaatattg aggtagcttt
     2881 cgcggctctc gggtccgccc acccgatacg cctcgtacgc gtgcgcgacg tggagcgcgg
     2941 tgcggtccgc gtcggagtag atcgccaccg gcgtgatccc catctccttg cacgcgcgtg
     3001 ccacccgaac ggcgatttcg ccgcggttgg cgatcaggat cttggcgaac atggcaatgg
     3061 gcctcggcgt cagagcggga tgttgccgtg cttcttgggc ggcatgctct ggcgtttgtt
     3121 cacgaggctc tcgagagcgg cgatgatctt ggggcgcgtg tccgcgggct cgatcacgtc
     3181 gtcgatgtaa ccgagcgacg cggcgatgta cgggttggcg aatttctcgt tgtactcttg
     3241 cacacggcgt gcgcgctccg cggcggggtc cttcgcggat tcgatctcgc gcttgttgag
     3301 gatattgacg gccccctcgg cgcccatcac cgccagctcc gcgctcggcc acgccacgtt
     3361 gtagtccgcg cgaatgtgct tcgaactcat gacgtcgtac gccccgccgt aggccttgcg
     3421 ggtgatgacc gtgatcttgg ggacggtggc ttcgcagtac gcgaacaaga gcttcgcgcc
     3481 gtgcttgatg atgccccccc actcctgctc ggtccccggc aaaaatcccg gcacgtcctc
     3541 gaaggtgacc aggggaatgt tgaacgcgtc gcagaagcga acgaagcgcg cggccttgat
     3601 cgacgacttg atgtcgagga cccccgcgag cgacttgggc tggttggcca ccacgccgat
     3661 ac
//
LOCUS       DASPGK010000172         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1331967, whole genome shotgun sequence.
ACCESSION   DASPGK010000172 DASPGK010000000
VERSION     DASPGK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331967"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VEC56_04955"
     CDS             complement(<1..940)
                     /locus_tag="VEC56_04955"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HXV13534.1"
                     /translation="MKTPNQARGGLFTAVAALTIAGPAQGVGQTISDSEALARVPMTF
                     TRNQVRIPVSVNGSEPFTLILDTGMPTRGLLLRAGERVDALGLEFLGTDSVSGGGSDG
                     ALSARVSKGQRIELGGLAIPEVPVIVLPEQNLMQDVDGVIGAELFERFAVRVDADRLR
                     VELIDPGIFQPAHGSSIVPLRFNQGVAFVDAEVTVDAGAPVAADLALDLGAGHALWLN
                     EKSNPRLASPAGAIETVLGRGLSGEIRGSIGRVHRLQLGDFAFENVVTLFPVGEHQNP
                     GGFDFRDGFIGAEILTRFVVTYDYAQKRMVLERGGMF"
     gene            1079..2410
                     /locus_tag="VEC56_04960"
     CDS             1079..2410
                     /locus_tag="VEC56_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13535.1"
                     /translation="MPRRLTPVAACAALFIAAAPSVRATGFDLVGYLDPSASNNYTDV
                     YGWSHPSNGRSYALVGNNATGLHIVDVSDPTNPFQVAVATTVPRYDTKTFGNLAYTVD
                     GLPGGVGGILDLSNPGSPVAVGSFTGGHNLWIDSKGFMYVALPGLTCYDLNGDPTVPA
                     FAWDIPGVDGHDSYVDGDILFDFRGHAGTFIYDVSDRYSPQGLGSITDPLIEFHHQGR
                     LTADGRYLYLCDEFAVSPQPDITIWDLAAIWNPVKVGAIYDPSATVHYCYVVGNTLAV
                     AYYTAGFKLYDITIPAWPVLVEQYDTSPMTGEGVYVGAYGCYPFGPGGTMYVSDRPNG
                     LYVFEMDFATGIERSPAGMPSVGPNTPNPFGASTTVPIRLARSEYASVAVYDARGARV
                     RELRAGFTTAGEHALEWDGRDDAGRAVASGVYFCRVRAGGREAVRKLVLVR"
     gene            complement(2499..>3173)
                     /locus_tag="VEC56_04965"
     CDS             complement(2499..>3173)
                     /locus_tag="VEC56_04965"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HXV13536.1"
                     /translation="GNYAFHRLPAGVYTVSIPPKPGIEPVTPTEITVLLTETEGDVSD
                     FLDANFGVLASYVPPDPFPIGAYIEANGEYATDPDRLVAQGIELARCGHNPYSPFDGG
                     DDDDDDGDNDDYDDERDCRKGKLRGPVTAVDDENGAFAVMGTWVSIDPTTTAYEVEVG
                     QRLDVRVHRADDGSLVADVIKPWHSDKEQVHGRIDEVEIGDDGVVQLKVLDTLVIVTR
                     HPHVTP"
BASE COUNT          512 a         1068 c         1039 g          554 t
ORIGIN      
        1 agaacatgcc tccccgttcg agtaccatgc gcttctgggc gtagtcgtaa gtcacgacga
       61 agcgagtcaa gatttccgcg ccgatgaaac cgtccctgaa atcgaagcca cccggatttt
      121 ggtgctcgcc gacggggaat agcgtcacca cgttctcgaa cgcgaagtcg cccagctgga
      181 gtcggtgcac gcgcccgatg gagccgcgaa tctcgcccga gaggccgcgg ccaagcacgg
      241 tctcgatcgc gcccgcgggc gacgccaggc gcgggttgct cttttcgttc agccacagcg
      301 cgtggccggc gcccaggtcg agtgcaagat cggcggcgac gggagcaccg gcgtcgacgg
      361 taacctcggc gtccacgaat gcgactccct ggttgaagcg aagcggcacg atcgaactcc
      421 cgtgggcggg ctggaagatc cccgggtcga taagctcgac ccgcaaacgg tcggcgtcga
      481 cccgcaccgc gaagcgctcg aacaactccg cgccgatgac gccgtcgacg tcttgcatca
      541 agttctgctc gggcaacacg atcacgggca cctcggggat cgcgagtccg cctagttcga
      601 tgcgttgccc cttcgacacc cgcgcgctga gggcgccgtc gctcccgccg cccgaaaccg
      661 agtcggtgcc gagaaactcc agccccagcg cgtcgacgcg ctcgccggcg cgcagcaaca
      721 agccgcgggt cggcatgccg gtgtcgagga tgagcgtgaa cggctccgag ccgttgaccg
      781 aaacgggaat gcggacttgg ttgcgcgtga aggtcatggg cacacgcgcc agggcttcgc
      841 tgtccgagat ggtctggcca accccctggg ccgggccggc gatggtgagt gccgcgacgg
      901 cggtgaagag gccgccccgg gcctggttcg gtgttttcat ggcggcaaaa tagggcgagg
      961 cgcacctacg gtcaagcgcc atttagtacg aatcgtacta agaaggggac catccatccg
     1021 tgttatcatg gtagctcgcc gccctcacca tcgtccctca tcccgaacgg cggtcaagtt
     1081 gcctcgtcgt ctcacacccg tcgccgcctg cgcggcgctg ttcatcgcag ccgccccgag
     1141 cgttcgtgcg accggcttcg acttggtcgg ctacctcgat ccctcggcgt cgaacaacta
     1201 caccgacgtg tacgggtgga gccacccgtc caacgggcgc tcgtacgccc tcgtcggcaa
     1261 caacgccacc ggtctgcata tcgtcgacgt gagcgatccc accaatccgt ttcaggtggc
     1321 ggtcgcgacc accgtgcccc gctacgacac caagaccttc ggcaatctgg cctatacggt
     1381 ggacgggctg cccggtggcg tgggtggcat cctcgatctc tccaacccgg ggagcccggt
     1441 cgcggtcggg agtttcaccg gcgggcacaa cctctggatc gattcgaagg gattcatgta
     1501 cgtcgccctc cccggcctca cctgctacga cctgaacggc gacccgaccg tgcccgcatt
     1561 cgcgtgggac atcccgggtg tcgatggcca cgattcgtac gtcgacggcg atattctttt
     1621 cgactttcgc ggccacgcgg gcacattcat ctacgacgtc agcgaccgct attcgcccca
     1681 gggactgggg tcgatcaccg acccgctgat cgagttccac caccaaggcc ggctcacggc
     1741 cgacgggcgc tacctctatt tgtgcgacga attcgcggtg tcgccgcaac ccgacatcac
     1801 catctgggat ctggcggcca tctggaatcc ggtcaaggtg ggcgcgatct acgacccgag
     1861 cgcgaccgtg cactactgct acgtggtggg gaacacgctc gcggtcgcgt actacacggc
     1921 cggtttcaag ctctacgaca tcacgattcc ggcctggccc gtgctggtgg agcagtacga
     1981 cacctcgccc atgacgggcg agggggtcta cgtgggcgcg tacgggtgct atccgttcgg
     2041 gccgggcggg acgatgtacg tcagcgatcg ccccaacggg ctctacgtgt tcgagatgga
     2101 cttcgcgacc ggcatcgagc gctctcccgc gggcatgccc tccgtgggcc ccaacacgcc
     2161 caaccccttc ggtgcttcga cgaccgtacc gattcgtctc gcgcggtccg agtacgcctc
     2221 ggtcgcggtg tacgacgcgc gcggcgcgcg cgtgcgcgag ctgcgtgcgg gcttcaccac
     2281 ggcgggcgag cacgcgctcg agtgggacgg gcgcgacgat gccgggcgcg cggtggcgtc
     2341 gggggtctat ttctgccggg tgcgcgcggg cgggcgcgag gcggtccgga agctggtgct
     2401 cgttcgatga gcgcgcgcga aacaagaaga ccgtccggag cggcctgctt gcccagtcgc
     2461 cccgaacggt cttccgtttg tcgctatgac aaactctctt atggcgtgac gtgcgggtga
     2521 cgcgtcacga tcaccaacgt atcgagcacc ttcaactgaa cgacaccgtc gtcgccgatc
     2581 tcgacctcgt cgatgcggcc gtgtacctgc tccttgtcgc tgtgccacgg cttgatcacg
     2641 tcggccacca gcgagccgtc gtcggcgcgg tggacgcgca cgtcgaggcg ctggcctact
     2701 tcgacctcgt acgcggtcgt cgttggatcg atcgacaccc acgtgcccat gaccgcgaag
     2761 gcgccgttct cgtcgtcgac ggcggtcacc gggccgcgca gcttgccctt acgacaatcg
     2821 cgctcgtcgt cgtagtcgtc gttgtctccg tcgtcgtcgt catcgtcgcc gccgtcgaag
     2881 ggagagtacg ggttgtgtcc gcaccgcgcc agctcgatgc cttgggcgac gagacggtcg
     2941 gggtcggtgg cgtactcgcc gttggcctcg atgtacgcgc cgatcgggaa cgggtcgggc
     3001 ggcacgtatg acgcgagcac gccgaaattg gcgtcgagga agtcggatac atcgccctcg
     3061 gtctcggtca agagcaccgt gatctcggtc ggtgtgaccg gctcgatgcc cggtttgggc
     3121 ggtatcgaca ccgtgtagac gcccgccggc aggcgatgga acgcatagtt ccc
//
LOCUS       DASPGK010000173         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1332277, whole genome shotgun sequence.
ACCESSION   DASPGK010000173 DASPGK010000000
VERSION     DASPGK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332277"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1127
                     /gene="purT"
                     /locus_tag="VEC56_04970"
     CDS             3..1127
                     /gene="purT"
                     /locus_tag="VEC56_04970"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HXV13537.1"
                     /translation="MGREVVISAKRLGKSVVAVDRYAGAPAMQVADCFEVINMLDAGE
                     LGRVVAKHRPDMIVPEIEAIRTERLRDFERNGITVIPTAEATYLTMNRVAIREVASKE
                     LALRTARYAYATTLDELRAAAVDVGIPCVVKPVMSSSGKGQSTVGTADQLGAAWDYAC
                     SNQRGDEKKVIVEEFVRFHLEITLLTIRQWDGPTLFVSPVGHRQERGDYMESWMPAEI
                     TSAQLEEAQRMAKKVTDRLGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTMISQDL
                     SEFDLHVRAILGLPIPTVTYYGASASAVILADRESPRIAGYEGLEKALAFPNSQVRIF
                     GKPDSRKYRRMGVALARAETVEEARTLAKQVADCVTIRYA"
     gene            1147..1887
                     /locus_tag="VEC56_04975"
     CDS             1147..1887
                     /locus_tag="VEC56_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV13538.1"
                     /translation="MKFTWLGGPSFVLELGPFRLVGDPVLADRVEVPGLGVVTRVKPA
                     PSPDALGVVADVSLVTSLRPDHFDPVALEKRTAGTVLAPAAAKLPTARVLSWGESIVL
                     EKAGTTLSITAVPAGVAAAPPPAERDNGYFLRLEGGAQPVTAYVTGDTRFSEATRELQ
                     LAYGYSNLLVLYIGAERAPDGSMRSLDSKDAMQIVYRMQPNAIAAVHHSTFSHYQESI
                     DPLIEKIELTIYDKRLRRLREGESFEKG"
     gene            complement(1910..2077)
                     /locus_tag="VEC56_04980"
     CDS             complement(1910..2077)
                     /locus_tag="VEC56_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXV13539.1"
                     /translation="MSESPFDRRWKMASVSADTAGGGAHRISIPYLPIAEARTVFDDL
                     GARVNETAFRW"
BASE COUNT          354 a          689 c          695 g          344 t
ORIGIN      
        1 agctggggag ggaagtcgtc atctcggcca agcggctggg caagtccgtg gtcgcggtcg
       61 accgctacgc gggcgcgccc gcgatgcaag tcgcggactg tttcgaagtg atcaacatgc
      121 tcgacgcggg cgagttgggg cgcgtggtcg ccaagcatcg ccccgacatg atcgtccccg
      181 agatcgaagc cattcgcacc gagcgactgc gcgatttcga gcgcaacggc atcacggtga
      241 ttccgaccgc cgaggcgacg tatctcacca tgaaccgcgt ggccatccgc gaagtggcgt
      301 ccaaggagct ggcacttcgc accgcgcgct acgcctacgc gaccacgctg gacgaattgc
      361 gcgcggccgc ggtcgacgtc ggcatcccgt gcgtggtcaa acccgtcatg tcgtcgtcgg
      421 ggaagggcca gtcgacggtt gggaccgcgg accagctggg cgctgcgtgg gactacgcgt
      481 gcagcaacca acgcggagac gagaagaagg tcatcgtcga ggaattcgtc cgctttcatc
      541 tcgagatcac gctgctcacg attcgccagt gggacggtcc cacgctgttc gtaagcccgg
      601 tcgggcaccg gcaggagcgc ggcgactaca tggagtcgtg gatgccggcc gagatcacgt
      661 ccgcgcagct cgaagaggcg cagcgcatgg cgaagaaggt gacggaccgc ctgggcggcg
      721 cgggcatctt cggcgtcgag ttcttcatca ccaaggacga agtgatcttc tccgagcttt
      781 cgccgcggcc gcacgacacc ggtatggtga cgatgatctc gcaggatctg tccgagttcg
      841 acctgcacgt gcgcgccatc ctcggactgc cgatcccgac cgtcacctac tacggcgcgt
      901 cggcgtcggc ggtgatcctg gccgaccgcg agtcgccgcg catcgcgggc tacgaaggcc
      961 tcgagaaggc gctcgcgttc ccgaattcgc aggtgcgcat cttcggcaag cccgactcgc
     1021 gcaagtaccg ccgcatgggc gtggcactcg cgcgcgcgga gaccgtggaa gaggcccgca
     1081 cgctcgccaa gcaagtcgcc gattgcgtga ccattcgcta cgcgtgacga ttcccctgac
     1141 acccgcatga agttcacctg gctcggtggt ccgtcgttcg ttctggagtt ggggccgttt
     1201 cgcctggttg gcgacccagt gctcgccgac cgcgtcgaag ttccgggact gggcgtggtg
     1261 acacgggtga aaccggctcc cagccccgac gcgctcggcg tcgtcgccga cgtgagcttg
     1321 gtcacctctc tccggcccga tcacttcgac ccggtggcac tcgagaagcg cacggcgggg
     1381 acggtgcttg ctcccgccgc cgccaagctg ccgaccgccc gcgtgctttc gtggggcgag
     1441 tcgatcgtgc tggaaaaagc cgggaccacg ctgtcgatca ccgcggtgcc cgcgggtgtg
     1501 gccgccgcgc caccgcccgc cgagcgcgac aacggatact tcctacgact cgaaggaggc
     1561 gcgcagcccg tcaccgccta cgtcaccggc gacacgcgct tctcggaggc gacgcgcgag
     1621 ctacagctcg cgtacggtta ctcgaacttg ctcgttctct atatcggcgc cgagcgcgcg
     1681 ccggacggtt ccatgcgcag cctggattcc aaggacgcga tgcagatcgt gtatcgcatg
     1741 cagcccaacg cgatcgcggc cgtccatcat tccaccttct cccactatca ggagtcgatc
     1801 gacccgctga tcgaaaagat cgagcttacg atctacgaca agcgcttgcg ccgattgcgg
     1861 gaaggcgagt cgttcgagaa gggatagggc ctcgccggcc ggtcacgagt taccagcgga
     1921 atgccgtctc gttgacgcgc gcgcccaggt catcgaagac ggtgcgggct tcggcgatgg
     1981 ggaggtaagg gatcgagatg cggtgcgcgc cgccgccggc cgtgtcggca gagacactcg
     2041 ccatcttcca gcgccgatcg aagggcgact cgctcatgtt ca
//
LOCUS       DASPGK010000174         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_865164, whole genome shotgun sequence.
ACCESSION   DASPGK010000174 DASPGK010000000
VERSION     DASPGK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865164"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VEC56_04985"
     CDS             complement(<1..265)
                     /locus_tag="VEC56_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13540.1"
                     /translation="MAWEDNRAANEDIYVQRITSAGVPQWTANGVALCVAVNGQYLPS
                     IESDGAGGAIVTWRDLRTPANGYDIYARRVNSAGTPLWTANGVA"
     gene            complement(645..1079)
                     /locus_tag="VEC56_04990"
     CDS             complement(645..1079)
                     /locus_tag="VEC56_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HXV13541.1"
                     /translation="MPPSAPPTDVVHEYPYDSVTTPEARGNHVVELAEQFLGTPYRSG
                     GTTSNGVDCSGLTYAVFREVGVTLPRGSDDQARVGAPVTRDELAPGDLIFFGSSSNVS
                     HVGIYAGGGEFIHASTRSRSVRFDKLETTYFRDRYLTARRVL"
     gene            1084..1326
                     /locus_tag="VEC56_04995"
     CDS             1084..1326
                     /locus_tag="VEC56_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13542.1"
                     /translation="MAVCVGADRGTDGSTGDGRPVKRGALEHAENVAAQIARRASLVA
                     EYRESLRIMNRQSGKERALHMPQCPIPVDTRAPCFV"
     gene            1370..>2302
                     /locus_tag="VEC56_05000"
     CDS             1370..>2302
                     /locus_tag="VEC56_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXV13543.1"
                     /translation="MSLSIQRAAVLGSGVMGSALAAHLANAGIPTLLLDIVPPAGAKV
                     KGDPGSREYRDAFARAGLERALKSKPAAFFSPARARRVAVGNLEDDVAQLRDCDWILE
                     AVVEKLDIKRALFEKIAPHVKDGAIVTTNTSGLSITDMAAVLPESMRPRFLGTHFFNP
                     PRYLHLLELIPHAGTDPAVLEFVREFGDAVLGKGVVVAKDTPNFIANRIGTFAVMSAI
                     RAMIEGNYTVEEADALTGPLIGRAKSATFRTADLVGLDILVHASTTVYDRATNDERRD
                     TFKPPALLEKMLKAGMLGDKTGKGFYQKVAGKTGS"
BASE COUNT          394 a          749 c          790 g          369 t
ORIGIN      
        1 gcgcgacccc gttggccgtc cacaacggcg ttcccgcgga gttcacgcga cgggcgtaga
       61 tgtcgtagcc gttcgcaggc gtgcgcaggt cgcgccacgt gacgatggcg ccacccgcac
      121 cgtcggactc gatcgacgga aggtactgtc cgttgacggc gacgcacagc gcgacaccgt
      181 tggcggtcca ctgcgggaca cccgcggacg tgatcctctg aacgtagatg tcctcgttgg
      241 cggcacggtt gtcctcccac gccacgatcg cgcccccccg cgccgtcgga cacgagcacg
      301 gggccttcct gcgcattgat cgcaacgcag atcgggacgc cgtcgaccgt ccacagggga
      361 acacccgcgg cgctgatgcg ctggacgtag atgtcgtcgt cgccgtttcg actgtcggca
      421 aacgccaaga tcgccccgcc ggatccgtcg ggaatcatcg atgtcagcac cggtccgccg
      481 ggcgacaggg taaggggatt tccgtcgagc tgccattcgg cctgggcgcc gaagggtgta
      541 atgaccaagg cgagcgcggc gaggacgggt gtcggcttca tcgtttcacc tctggggttt
      601 caacgagtct accggactcg cggtgcgact ggcaatgaag gggtttacag aacgcgccgg
      661 gcggtcaggt agcgatcgcg gaagtaggtg gtttcgagct tgtcgaagcg aacgctgcgc
      721 gagcgcgtgg aggcgtggat gaactcgccg ccgcccgcgt aaatgccgac gtgcgagacg
      781 ttgctgctcg atccgaagaa gatcaagtcg ccgggtgcca actcgtcgcg ggtgacggga
      841 gcgcccacgc gcgcttggtc gtcggagccg cgagggagcg tgacgccgac ttcgcgaaat
      901 acggcgtacg tcagcccgga gcagtctacg ccgttactcg tcgtcccgcc cgagcggtac
      961 ggtgttccga gaaactgctc ggccagctcg accacgtggt tgccgcgcgc ctcgggtgtc
     1021 gtgacggagt cgtagggata ctcgtgcacg acgtcggtcg ggggcgcgga cggaggcacg
     1081 cgcgtggcgg tctgcgttgg cgcggaccgc ggcaccgacg gctcgaccgg cgacgggcgg
     1141 ccggtgaagc ggggcgcgct cgagcacgcc gagaacgtcg ccgcgcagat cgcgagaagg
     1201 gcgagcctcg tcgcggaata tcgggaaagc cttcggatca tgaaccgcca aagtggcaag
     1261 gaacgtgcct tgcatatgcc gcaatgccca atcccggttg acacgcgggc gccgtgcttc
     1321 gtatagctag cggtgtcggg ctatttcgag gaacgataag gagtttccgt tgtccctctc
     1381 gatccaacgc gccgccgtac tgggctcggg cgttatgggg agtgccctcg ccgcgcacct
     1441 cgcgaacgct ggaatcccta cactgctgct cgacatcgtt ccacccgccg gggccaaggt
     1501 caagggcgac ccgggctcgc gcgagtaccg cgacgccttc gcgcgcgccg gcctggagcg
     1561 cgccctcaag tcgaagccgg ccgcgttctt ttcgcccgcc cgcgcgcgcc gcgtggccgt
     1621 gggcaacctc gaggacgacg tcgcccaact ccgcgactgc gactggatcc tcgaagccgt
     1681 ggtcgagaaa ctcgatatca agcgcgcgct gttcgagaag atcgcgcccc acgtcaaaga
     1741 cggcgcgatc gttaccacca acacgtcggg gctttcgatc acggatatgg cggcggtgct
     1801 gccggaatcg atgcgcccgc gcttcctcgg cacgcacttc ttcaacccgc cgcgctatct
     1861 ccatctgctc gagctgatcc cgcacgcggg caccgacccc gccgtgctgg agttcgtgcg
     1921 cgagttcggc gacgccgtgc tcggcaaggg tgtggtggtc gcgaaggaca cgcccaattt
     1981 tatcgccaac cgaatcggaa cgttcgcggt gatgtccgcg atccgcgcca tgatcgaggg
     2041 aaactacacc gtcgaagagg ccgacgcgct gacgggaccg ctgatcgggc gcgcgaagag
     2101 tgcgaccttc cgcaccgccg acctggtcgg tctcgacatc ctggtgcacg cgtcgaccac
     2161 cgtgtacgac cgcgcgacca acgacgagcg acgcgacacc ttcaagccgc cggcgttgct
     2221 ggaaaagatg ctcaaggccg gcatgctggg cgacaagacg gggaagggtt tctatcagaa
     2281 ggtcgcgggg aagacgggtt cg
//
LOCUS       DASPGK010000175         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1449833, whole genome shotgun sequence.
ACCESSION   DASPGK010000175 DASPGK010000000
VERSION     DASPGK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449833"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VEC56_05005"
     CDS             complement(<1..491)
                     /locus_tag="VEC56_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HXV13544.1"
                     /translation="MTDEPGRRYPVPARGVRVEDRIEKSRFIASAARAESAREARAFV
                     DALRAEFPDATHHCFAFVAGPPGSTSAVGSSDDGEPAGTAGRPMLSVLLNSGVGEIVV
                     VVTRYFGGVKLGKGGLVRAYTGAVQHVLRELETMERVTLIEVRVVVSYAQADAVRRAL
                     ERAG"
     gene            complement(484..858)
                     /locus_tag="VEC56_05010"
     CDS             complement(484..858)
                     /locus_tag="VEC56_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011705627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV13545.1"
                     /translation="MKHSKRFLEIVKAVKARVKETNVQTIRARQKKGDTFYLVDVRED
                     NEWDKGHLPGAIHLGKGVIERDVEGVIPDVDAEIVLYCGGGYRSALAAENLARMGYTN
                     VVSMDGGFRGWTEAGFPIEHDD"
     gene            complement(855..1541)
                     /locus_tag="VEC56_05015"
     CDS             complement(855..1541)
                     /locus_tag="VEC56_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXV13546.1"
                     /translation="MDARARSLHVSDAAGDVSALVAVPRDAWAGYVLAHGAGAGMRHA
                     FLEEVAARLYDAGVATLRYQFPYMEGRKGRPDPPAVAEKTVEAAVACAATELAGLPLF
                     AGGKSFGGRMTSQAASRAALAGVRGLVFLGFPLHAPKRPSVARAAHLSSVTVPMLFVQ
                     GTRDDLASMDLMREVCASLGNRAMMHVVEDGDHSFKVLKRTGRTHEQVMEGIRDAAVA
                     WMRRVLKGAS"
     gene            complement(1554..2309)
                     /locus_tag="VEC56_05020"
     CDS             complement(1554..2309)
                     /locus_tag="VEC56_05020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV13547.1"
                     /translation="MQIDLSRQRALVTGASRGIGRAIAAALMDSGACVVAHYHRERAG
                     AEALVRGRDGSIALAADLGDGRAAASLVARAVDALGGLDLLVNNAGIALESPLDSSTE
                     DWLRAWDATMAVNLTASGVLSRDAVRHFAAHGGGRIVFIASRAAFRGDTPEYLAYAAS
                     KGGMVALARSIARGCGEQGVKSFVIAPGFTRTEMADDFIARYGEAYAMKDVALDRMTE
                     PRDIAPLVVFLASGLGDHCTGTSIDVNAASYVR"
     gene            complement(2356..2868)
                     /locus_tag="VEC56_05025"
     CDS             complement(2356..2868)
                     /locus_tag="VEC56_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13548.1"
                     /translation="MVQTLVRKVLGAIAGVAICIAIWTIQDKLTGGGSESTDSIPHEV
                     WGGGAGTVTLEVEASEPAFVSASFETNSYSLGDDGHEYLESWQKIPAGKHTFEIDVPA
                     DVSGSVWVRVDEPSVGAKVKLVLRANGQVVGEDAMTLEKPLEDGYGFAAGLEVDDYAK
                     GKLTDEGFFD"
     gene            complement(3055..3540)
                     /locus_tag="VEC56_05030"
     CDS             complement(3055..3540)
                     /locus_tag="VEC56_05030"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HXV13549.1"
                     /translation="MAHKSFTVGEANALVSALTQTFRQIDAHKYQIREAGKKIEVLEL
                     LWGDALRDPAHTDHAEFMTHRETVDRCLRGIQRCVQEGVVALGLRLPMGGIEDGLVDF
                     PTTYEGRWVYLCWHVGERELTHWHEIDAGYQGRQPITASQREAMGRDDPSTIDDSELD
                     F"
     gene            complement(3544..4581)
                     /gene="mtnA"
                     /locus_tag="VEC56_05035"
     CDS             complement(3544..4581)
                     /gene="mtnA"
                     /locus_tag="VEC56_05035"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXV13550.1"
                     /translation="MAPVEALPTVRFADGSVRIIDQTLLPARYEIVSLATVDAVCEAI
                     RSLRVRGAPAIGIAGAFGILVALSEARTKSLDDLREGLADATRRIGATRPTAVNLAWA
                     LDRMRYVWENATDARALPDALAREAHAILEEDLAMSAAMTSHGATLLRSADAVLTHCN
                     TGGLATGGGGTALGAVFAAVGEGRRIHVFADETRPLLQGARLTAWECAQRGIPVSVLV
                     EGAAASLLTSGRVAAVLVGADRIAANGDVANKVGTLGLAIMARRAGVPFYVVAPSSSI
                     DPSRASGVDIPIEHRAHDEVVRFGGLATAPEGIDAYNPAFDVTPAELIDAIVTERGVH
                     RPPYVFARSRG"
     gene            complement(4585..5175)
                     /locus_tag="VEC56_05040"
     CDS             complement(4585..5175)
                     /locus_tag="VEC56_05040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXV13551.1"
                     /translation="MNPFLPPGQQKDLVLASRSPRRVEIMRGLGFEFDVEPAPEHLED
                     GFDHDDPFAIPQILAARKCVHVAERRPKSLVIAADTVVIIDDRILNKPANDAEARAFL
                     RSLAGRTHVVVTGLAVVGHERGVHIEKSERTFVTFRELSDDEIARYVATGEGRDKAGS
                     YAAQGVGAGLIRAIDGCFFNVVGLPVALLIDMLREV"
     gene            complement(5172..>5401)
                     /locus_tag="VEC56_05045"
     CDS             complement(5172..>5401)
                     /locus_tag="VEC56_05045"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXV13552.1"
                     /translation="VVSQFTLYGDVGKGARPSFDDSAPPEVARALYDDFVAALRALLP
                     GRVHTGEFQATMQVSLVNDGPVTILVEKEAP"
BASE COUNT          836 a         1900 c         1835 g          830 t
ORIGIN      
        1 cccgcgcgct cgagcgcgcg ccgaaccgca tcggcctggg catacgaaac aaccacgcgc
       61 acttcgatca gcgtgacgcg ttccatcgtc tccagctcgc gcagcacgtg ctgcaccgcg
      121 cccgtgtacg cgcgcaccag cccccccttg ccgagcttga caccgccgaa gtagcgcgtc
      181 accaccacga cgatctcgcc cacgccggag ttcaagagaa ccgagagcat gggacgcccc
      241 gccgtgcccg cgggctcgcc gtcgtcgctc gagcccaccg ccgaggtgct cccgggcggg
      301 cccgcgacga aggcgaaaca atggtgggtg gcgtcgggga actcggcgcg cagggcgtcg
      361 acgaaggcgc gcgcctcccg cgcggactcc gcgcgcgccg cgctcgcgat gaagcggctc
      421 ttctcgatgc ggtcttcgac gcgcacgccg cgcgcgggaa ccgggtagcg ccggccgggc
      481 tcgtcagtca tcgtgctcga tcggaaagcc ggcttcggtc caaccgcgga acccgccgtc
      541 catcgagacc acgttggtgt agcccatgcg cgccaggttc tccgcggcca gcgcggagcg
      601 atagccgccg ccgcagtaca acacgatctc cgcgtcgacg tcggggataa caccttcgac
      661 gtcgcgctcg atcaccccct tgcccaagtg gatggcgccc ggcaggtgcc ccttgtccca
      721 ctcgttgtcc tcgcgcacgt ccacgagata gaaggtgtcg cccttcttct ggcgcgcgcg
      781 gatcgtttgc acgttggtct ctttcacgcg cgccttgacg gccttgacga tctcgaggaa
      841 acgcttcgaa tgcttcatga tgctcctttt aggacgcgac gcatccatgc cacggcggcg
      901 tcgcgaatcc cttccatgac ttgctcgtgc gtgcgaccgg ttcgcttcag caccttgaac
      961 gaatggtcac cgtcttcgac cacgtgcatc atcgcgcgat ttccgagcga cgcgcacacc
     1021 tcgcgcatca ggtccatcga cgccaagtcg tcgcgggtgc cttgaacgaa cagcatcggc
     1081 accgtcacgg acgacaggtg cgcggcccgc gcgacactcg ggcgtttggg cgcgtgcagc
     1141 ggaaagccga ggaagacgag tccgcgcaca cccgccagcg cggcccgcga cgccgcctgc
     1201 gacgtcatgc gcccgccgaa cgacttgccg cccgcgaaca gcggcaaccc ggccagctcg
     1261 gtcgctgcac aggcgacggc cgcttccacc gtcttctccg ccaccgccgg cgggtccggg
     1321 cgccccttgc gtccctccat gtacgggaac tggtagcgga gcgtcgccac accggcgtca
     1381 tacaaccgcg cggccacctc ttcgagaaac gcgtgccgca tccccgcccc cgcgccgtgc
     1441 gcgagcacgt agccggccca cgcgtcgcgc ggcacggcca ccagcgcgga cacgtcgccc
     1501 gcggcgtccg aaacatggag acttcgcgcg cgcgcgtcca tgccgtcgcg cgatcagcgc
     1561 acgtagctcg ccgcgttgac gtcgatcgac gtccccgtgc agtggtcgcc aagcccgctc
     1621 gcgaggaaga ccacgagtgg cgcgatgtcg cgcggctcgg tcatgcgatc gagggccacg
     1681 tccttcatgg cgtaggcctc gccgtagcgc gcgatgaagt cgtcggccat ctcggtgcgc
     1741 gtgaatccgg gagcgatcac gaacgacttg accccttgct cgccgcagcc gcgggcgatc
     1801 gagcgcgcga gggcgaccat gccgcccttg gaggccgcgt aggccaggta ttcgggggtg
     1861 tcgccgcgaa acgcggcgcg cgaggcgatg aacacgatgc gaccgccgcc gtgggcggcg
     1921 aagtggcgca cggcgtcgcg cgagagaacc cccgacgcgg tgaggttcac cgccatcgtg
     1981 gcgtcccacg cgcgcagcca gtcttcggtc gatgaatcga ggggagactc gagcgcgatg
     2041 cccgcattgt tgacgagcaa gtcgagtccg ccgagggcat cgacggcgcg cgcgacgagc
     2101 gacgcggcag cgcgcccatc gccgaggtcg gccgcgagcg cgatcgaccc gtcacggccg
     2161 cgcaccagcg cttcggcgcc ggctcgctca cggtgatagt gggcaaccac acacgcgccg
     2221 gagtccatca gggccgcggc aatcgcgcgt ccgataccgc ggctggcacc cgtgaccagc
     2281 gcccgctgtc gggagagatc aatctgcatg cgggcgatgg tagcgcaacc gcgaccggtg
     2341 cgctaccgcg cccgatcagt cgaagaagcc ttcgtccgtc agcttgccct tggcgtagtc
     2401 gtccacctcg agcccggccg cgaaaccgta gccgtcttcg agcggcttct cgagcgtcat
     2461 ggcgtcttcg ccgacgacct ggccgttggc gcgcaggacc agcttgacct tggcgcccac
     2521 gctgggctcg tcgacgcgca cccacaccga gcccgacacg tcggccggga catcgatctc
     2581 gaaggtgtgc ttgccggcgg gaatcttctg ccaggattcc aggtactcgt ggccgtcatc
     2641 gccgagcgag taactgttgg tctcgaacga cgcggaaacg aaggcgggct cgctggcctc
     2701 gacctcgagg gtcacggttc cggctccccc gccccagacc tcgtgcggga tggagtcggt
     2761 ggactcgctg ccgccgccgg tcagcttgtc ctggatggtc caaatcgcga tacagatcgc
     2821 gacaccggcg attgcgccga gcactttgcg aactagggtc tgcaccatat tggcacctca
     2881 atgctttatg tgacacacca agggacccaa ctaacccgtt gtcgattcgt gagaacgcgc
     2941 aggcggcggg ctgggcaaat cgtgccgccc ccgcgcgcgc tcgagcgccg caggtccttg
     3001 gtccgggtcc gcgctgcgcc ttgcgggttt tgtgccggac ggggtggagg ttgactagaa
     3061 gtccagctcg gagtcgtcga tcgtagacgg gtcgtcgcga cccatcgcct cgcgttggga
     3121 cgcggtgatg ggctggcggc cctggtagcc agcgtcgatc tcgtgccagt gggtaagctc
     3181 gcgctcgccg acgtgccagc acagatagac ccagcgccct tcgtacgtgg tcggaaagtc
     3241 gaccaggccg tcttcgatgc cgcccatcgg gaggcgtagt cccagtgcga ccacgccttc
     3301 ctggacgcag cgctggatgc cgcgcaggca gcggtcgacg gtttcgcggt gggtcatgaa
     3361 ttcggcgtgg tcggtgtgcg cggggtcgcg cagcgcgtcg ccccacaaga gctcgagcac
     3421 ctcgatcttc ttgccggctt cgcggatctg atacttgtgt gcgtcgattt gacgaaacgt
     3481 ttgcgtcagc gcggacacga gcgcgttggc ttcgccgacg gtgaacgatt tgtgagccat
     3541 cggctacccc cgcgatcgcg cgaagacgta gggcgggcgg tgcacgccgc gctcggtgac
     3601 gatggcgtcg atcaactcgg ccggtgtcac gtcgaaggcg gggttgtacg cgtcgatgcc
     3661 ttcgggtgcg gtggcgagtc cgccgaagcg cacgacttcg tcgtgggcgc gatgctcgat
     3721 gggaatgtcc acgccggagg cgcgcgacgg gtcgatcgag ctcgacggtg cgacgacgta
     3781 gaacggcacg ccggcgcgtc gcgccatgat cgcgagcccg agcgtcccga ccttgttggc
     3841 cacgtcgccg ttggccgcga tgcgatccgc acccacgagc acggccgcca cgcggcccga
     3901 cgtgagcagc gacgccgccg ccccttcgac cagcaccgag acgggaatcc cgcgctgcgc
     3961 gcactcccac gcggtcaagc gcgcgccttg caggagtggg cgcgtttcgt cggcgaacac
     4021 gtggatgcgc cggccttctc ccaccgccgc gaacacggcg ccgagggcgg ttcccccgcc
     4081 cccggtggcc aggccgccgg tgttgcagtg ggtgaggacg gcgtccgcgc tgcgcaggag
     4141 ggtggcgccg tgcgacgtca tcgcggcgct catcgccagg tcttcctcga ggatcgcgtg
     4201 cgcttcgcgc gcgagtgcgt ccggcagcgc gcgagcgtcg gtcgcgtttt cccacacgta
     4261 acgcatgcga tcgagcgccc acgccaggtt gaccgccgtc ggtcgggtgg caccgatgcg
     4321 tcgtgtcgca tcggccaacc cctcgcgcag gtcgtcgagg ctcttcgtgc gtgcctccga
     4381 cagcgcaacc aggatcccga aggcccccgc gatcccgatc gccggggctc cgcgcacgcg
     4441 cagcgaccgg atcgcctcgc acaccgcgtc gacggtggcg agcgacacga tctcgtagcg
     4501 cgcgggcagg agcgtttggt cgatgatgcg aacgctcccg tcggcgaagc gaacggtcgg
     4561 aagggcttcg acgggcgcca tcgactacac ctcgcgcagc atgtcgatta gaagcgccac
     4621 cggcaggccg accacgttga agaagcaccc atcgatggcg cggatcagcc ccgcccccac
     4681 gccttgcgcg gcgtacgagc ctgccttgtc gcgcccctct ccggtggcga cgtaacgcgc
     4741 gatctcgtcg tcggagagct cgcgaaaggt cacgaaggtg cgctcgctct tctcgatgtg
     4801 aacgccgcgc tcgtgaccga ccaccgccag tcccgtcacc accacgtggg tgcgtccggc
     4861 gagcgagcgc aggaaggcgc gcgcctccgc gtcgttggcg ggcttgttga ggatgcggtc
     4921 gtcgatgatg acgaccgtgt cggcggcgat cacgagcgac ttcgggcgcc gctcggcgac
     4981 gtgcacgcac ttgcgcgccg ccaagatctg cggaatggcg aaggggtcgt cgtgatcgaa
     5041 accgtcttcg aggtgctcgg gcgcgggttc gacgtcgaac tcgaagccca gcccgcgcat
     5101 gatctcgacc cggcgcggcg agcgcgacgc gagcacgagg tccttctgct gtccgggcgg
     5161 gaggaacgga ttcatggcgc ctccttctcc acgaggatgg tcacagggcc gtcgttgacg
     5221 agcgagacct gcatggtggc ctggaactcg ccggtgtgca cgcgccccgg aaggagcgcg
     5281 cgcagcgcgg ccacgaagtc gtcatatagg gcgcgcgcca cctcgggcgg cgcgctatcg
     5341 tcgaagctcg gccgcgcgcc tttaccaacg tccccgtaga gtgtgaactg ggagacgacc
     5401 a
//
LOCUS       DASPGK010000176         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_514193, whole genome shotgun sequence.
ACCESSION   DASPGK010000176 DASPGK010000000
VERSION     DASPGK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514193"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VEC56_05050"
     CDS             complement(<1..237)
                     /locus_tag="VEC56_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13553.1"
                     /translation="MSETIIIAPPGRVSGDLVYSERVSRDRSLHVLMQDVSARADGER
                     RVFLSAAFRDAVARYHSAAQPERALAYLRRTVRAL"
     gene            complement(328..1092)
                     /locus_tag="VEC56_05055"
     CDS             complement(328..1092)
                     /locus_tag="VEC56_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXV13554.1"
                     /translation="MEQVKFIDYYEVLGVWPTADNDTIKKAYFKLAKLHHPDVVGEAA
                     GKDGVDFKLINEAFAVLSDTVKRREFDEKLKRKTGTPAERREADKRSAQLSYDQARAA
                     MRQNRYDKAAVLLKAAIKYDDSNPAYLSWYGFSLGVLRTQLHEARDACKKALEMEFYN
                     ADYHANLGFVYMQAGLAKTAMECFEEALKWDPDHPVALKYMKGGKDGKPFANGDGKSV
                     VGGLLGIFRKGDKPAEKPAKAGTGARKTPSGRPPRR"
     gene            complement(1132..2076)
                     /locus_tag="VEC56_05060"
     CDS             complement(1132..2076)
                     /locus_tag="VEC56_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13555.1"
                     /translation="MFLKRLFAFIPKDEFSKALNLFNSGEYRKALGRFQEIQAVAGSA
                     GDIDRATLDLYTCEAHVALAKEFDQAGDLDSAMREMEAAVSIKPMFADLHYKLGALYV
                     RKSRLEDACNCFRNSLDINNKFFRSRIYLSKVLRELGDKDAAIREAVSSKHSCPNFYR
                     ESLDTLVVALRNGNDEDIRRLFEEMIEERPSSAQISKELAVEAIQNGNMDEAIRELKK
                     AIVLKPDYPDLHNYLGIAYGNSGMVDDAVHEFEVALKINPYYAKARLNLALLYYENTR
                     FDEAQAQIDHVLKVQPDNQLANNLLSELRAVTSGKFGK"
     gene            complement(2081..2692)
                     /locus_tag="VEC56_05065"
     CDS             complement(2081..2692)
                     /locus_tag="VEC56_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HXV13556.1"
                     /translation="MSTPRHDAPEPKPMRHELQRKSFHLGMIVVPAAVYFLDPTPALL
                     GLIIATFVTVAIDLMRLGDHRLRRFFLQLFRPLIRRHEEEHLLGSTHYMIAALLSVVV
                     FDHEIAIAALMFLVLGDAAAAIIGKRFGKPLFWGKSPQGSIACFVVCLGLGWPLLRSP
                     ELAVIGALAATVAEAMPSPLDDNMRVPIFSGIAMQIAARFLQG"
     gene            complement(2805..>4670)
                     /locus_tag="VEC56_05070"
     CDS             complement(2805..>4670)
                     /locus_tag="VEC56_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXV13557.1"
                     /translation="EALVGLIDEERVSVLRALGRPADALASLEDGIAHAGDGPERRRL
                     LALRYHVALEAGDPALASTSAIALTEGYRSFPEAEACALEVTRPLRASGSAEGISTRL
                     LLACAEVFSARGRPDDARRVLRALDARRLSVEDAEHHRLLWGEYHYATGDPSRAIQLA
                     RPSYSSAEYKRRSMILLARGLRGAGRKADAAVIYERFAATYPNDQIAPDALYAAASLR
                     EQNGQPAESARLLDQLRRSYPSSFHGWAAAIRRANALQKGGDVDEAAAIFEQWLARSR
                     RTDEAALFYLSRLYESTSSGENASLLLDELRRVNPYSFYVAPDPGRDARGPMRDAMGT
                     MVRDGAGSLSAWLEGADTDRDRAYRRVLEAAAGEGDASGDPATRDALERGRWFLEAGF
                     RDWAERELEVVRRKPGISPELALSLARTYEEYAMPWRSVRAFDRARVGLPWEKRRNHA
                     DDFRYLMYPVPFPARVFDASAQSEVPAHLIYAIMREESRFETDAISRAGAVGLMQLMP
                     DTARRVAKRKELGADVDGRLDDPDINVAIGTWYASDLLRAGDGSVAWMLAAYNAGPIA
                     ARRWLEPGVSGDDAIAAVESIDYRETRGYVKRVVESANVYQALYFGASGAENSPR"
BASE COUNT          758 a         1569 c         1548 g          795 t
ORIGIN      
        1 gagggcacga acggtgcggc gcaggtacgc gagcgctcgc tccggttggg cggccgaatg
       61 gtagcgagcc acggcgtcgc ggaaggcggc ggagagaaaa acgcgccgct cgccgtccgc
      121 gcgcgcagaa acgtcctgca tgagcacgtg gagactgcgg tcgcggctca cgcgctcgga
      181 atagaccaga tcgccggaga cccgccccgg cggtgcgatg atgatggttt cgctcatgct
      241 aagggcgcgg gggcgtacca atgccgccgc gatcccgagc ctaggcgcgc gcgtttcgca
      301 gtgtcaaagg tttgtttgga aggaacgtta gcggcgcggc gggcgacccg agggtgtctt
      361 gcgggcgccg gtccccgcct tcgcgggttt ctcggccggc ttgtcgccct tgcggaaaat
      421 tcccaggaga ccgcccacca ccgacttgcc gtcaccgttg gcgaagggct tcccgtcctt
      481 gccgcccttc atgtacttca gggccaccgg gtggtcgggg tcccacttga gcgcttcttc
      541 gaagcactcc atcgccgtct tggccagccc cgcttgcata tacacgaagc ccaggttggc
      601 gtggtagtcg gcgttgtaga actccatctc gagcgccttc ttgcaggcgt cgcgcgcctc
      661 gtgcagctgg gtgcgcagca cgccgagcga gaacccatac caactcaggt acgcggggtt
      721 actgtcgtcg tacttgatgg ccgccttgag caacaccgcg gctttgtcgt atcggttctg
      781 tcgcatcgcg gcccgcgctt ggtcgtacga gagctgcgcc gagcgcttgt cggcttcgcg
      841 gcgttcggcc ggcgtccccg tcttgcgctt gagtttctcg tcgaactcgc ggcgcttgac
      901 ggtatcgctc aacacggcga acgcctcgtt gatcagcttg aaatcgacgc cgtccttgcc
      961 ggcggcctcc ccgaccacgt ccgggtggtg gagcttggcg agcttgaagt acgccttctt
     1021 gatggtgtcg ttgtcggccg tcggccacac acccaacacc tcatagtagt cgatgaattt
     1081 tacctgctcc atgcgtcgcc ttcaaaaccg cgccgttgcg cgtgggctcc cctacttacc
     1141 gaactttccg ctagtcaccg ctcgcagctc gctcagcaga ttgttggcga gctggttgtc
     1201 gggctggacc ttcagtacgt gatcgatctg cgcctgcgcc tcgtcgaagc gggtgttctc
     1261 gtagtagaga agcgccagat tcaagcgggc cttggcgtag tacgggttga tcttgagcgc
     1321 cacctcgaac tcgtgcacgg cgtcgtccac catgccgctg ttgccgtagg cgatgcccag
     1381 gtagttgtgc aaatccgggt agtcgggctt gagcacgatc gccttcttga gctcgcggat
     1441 cgcctcgtcc atgttgccgt tctggatcgc ctcgaccgcc agctccttgc taatctgcgc
     1501 cgagctgggt cgctcctcga tcatttcttc gaacaagcgc cggatgtcct cgtcgttgcc
     1561 gttgcgcagc gccaccacca acgtatcgag gctctcgcga tagaagttcg gacagctgtg
     1621 cttggaactg accgcctcgc gaatggcggc gtccttgtca cccagctcgc gcagcacctt
     1681 ggagaggtaa atgcgcgaac ggaagaactt gttgttgatg tcgagagagt tgcggaagca
     1741 attgcacgcg tcttcgagcc tgctcttgcg aacgtacagc gcccccagct tgtagtggag
     1801 gtccgcgaac atgggcttga tggagaccgc cgcctccatt tcgcgcatcg cgctatccag
     1861 gtccccggcc tggtcgaact ccttggccag tgcgacgtgc gcctcgcacg tgtacaagtc
     1921 gagcgtggcg cggtcgatgt cgcccgccga ccccgcaacg gcctggatct cctgaaaacg
     1981 cccgagcgcc ttccggtact cccccgagtt gaacaggttc aacgccttcg agaactcgtc
     2041 cttcggaatg aacgcaaaca gtcgcttgag aaacatgggc ctacccctgc aggaaccgtg
     2101 cggcgatctg catcgcgatg ccgctgaata tcggaacccg catgttgtcg tccagaggcg
     2161 acggcatcgc ttccgccacc gtcgcggcta gcgcgccgat caccgcgagc tcgggagacc
     2221 gcaagagcgg ccacccgagt cccagacaaa cgacgaagca cgcgatgctg ccctgcgggc
     2281 tcttgcccca gaacagcggc ttgccgaagc gcttgccgat gatcgcggcc gccgcgtctc
     2341 cgagcaccag gaacatgagc gccgcgatgg cgatctcgtg atcgaagacg acgacgctca
     2401 acagcgccgc gatcatgtag tgcgtcgacc ccagcaggtg ttcctcttcg tgtcgccgga
     2461 tgagcggtcg aaacagttgg agaaagaacc gccggagccg atggtcgccc aatcgcatga
     2521 gatcgatcgc gaccgttaca aaagtagcaa taatgaggcc aaggagagcc ggggtggggt
     2581 ccaggaagta gaccgccgcg ggcacgacga tcatcccgag atgaaacgac ttacgctgca
     2641 gctcgtgccg catcggcttg ggctcgggag catcgtgacg aggcgtgctc atgggacagg
     2701 aatgtagccg accttgggcc acacaggcaa ttctgactcg gggagaacgc gactcggcaa
     2761 acgtcgccgg ccggcccggg gccgggcgtg caaactgcac gcggctagcg aggggaattc
     2821 tcggcgcccg aagcgccgaa gtagagcgct tggtagacgt tcgccgactc cacgacgcgc
     2881 ttgacgtaac cgcgcgtctc ccggtagtcg atcgattcga cggccgcgat ggcgtcgtcg
     2941 ccggagacac ccggctcgag ccagcgtcgg gccgcgatcg ggcccgcgtt gtacgcggcc
     3001 agcatccacg ccacgctccc gtctcccgcg cgcagcaagt ccgacgcgta ccaggtaccg
     3061 atcgccacgt tgatatcggg gtcgtcgagg cggccgtcca cgtcggcgcc gagttccttg
     3121 cgcttggcga cccggcgcgc ggtgtcgggc atcagctgca tgagcccgac cgcgcccgcg
     3181 cgcgatatcg cgtcggtctc gaagcggctc tcttcgcgca tgatggcgta gatcaggtgc
     3241 gcgggcacct cgctctgcgc cgacgcgtcg aaaaccctgg ccgggaacgg cacgggatac
     3301 atgagatagc gaaagtcgtc cgcgtggttg cgccgcttct cccacgggag ccccacgcgc
     3361 gcgcgatcga acgcgcgcac gctccgccac ggcatcgcgt attcctcgta cgtcctcgcg
     3421 agcgagagcg cgagctcggg cgaaatgccg ggcttgcggc gcacgacctc gagttcgcgc
     3481 tccgcccagt cgcggaagcc ggcctcgagg aaccagcgcc cgcgctccaa cgcgtcgcgc
     3541 gtggcgggat cgccggacgc gtcgccctca cccgcggccg cctccagcac gcgccggtag
     3601 gcgcgatcgc ggtcggtgtc cgcgccctcc aaccacgccg agagcgagcc cgcgccgtcg
     3661 cgcaccatgg tgcccatcgc gtcgcgcatc ggtccgcgcg cgtcccgccc ggggtcgggc
     3721 gccacgtaga acgagtaggg attgacgcga cgcaattcgt ccagcagcaa cgatgcgttc
     3781 tcccccgagc tggtcgattc gtacaggcga gagagataga acaacgccgc ctcgtcggtc
     3841 cggcgcgagc gcgcgagcca ctgctcgaag atggcggccg cttcgtcgac gtcgccgccc
     3901 ttttgcagcg cgttcgcgcg ccggatcgcc gccgcccagc cgtggaagga cgacgggtaa
     3961 gagcgccgca gttggtcgag caagcgcgcg ctctccgcgg gctggccgtt ttgctcgcgc
     4021 aaggacgcgg ccgcgtagag cgcatcgggc gcgatttggt cgttggggta ggtggcggcg
     4081 aagcgctcgt agatgaccgc ggcatccgcc ttcctccccg cgccgcgcag tccgcgcgcc
     4141 aggaggatca tcgagcgccg tttgtactcg gcgctcgagt acgacggacg cgcgagctgg
     4201 atcgcgcgcg aggggtcacc ggtggcgtag tggtactcgc cccacaggag ccggtggtgc
     4261 tcggcgtctt cgaccgacag tcggcgagcg tcgagcgcgc gcagcacgcg acgcgcgtcg
     4321 tcgggacggc cgcgcgcaga gaacacctcc gcgcacgcga gcaagaggcg cgtcgagata
     4381 ccctcggcgc tgcccgacgc gcgcaacggt cgcgtcacct cgagcgcgca cgcctccgcc
     4441 tcgggaaacg agcggtagcc ttcggtcagc gcgatcgcgg aggtcgatgc gagcgcgggg
     4501 tcgcccgctt caagtgccac gtggtagcga agtgccagca gtcgccgccg ttcggggccg
     4561 tcgccggcgt gggcgatgcc gtcctcgagc gacgcgagcg cgtcggcggg ccggccgagt
     4621 gcgcgcagca cgctcacgcg ctcctcgtcg atcaatccca ccagcgcctc
//
LOCUS       DASPGK010000177         7932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_162299, whole genome shotgun sequence.
ACCESSION   DASPGK010000177 DASPGK010000000
VERSION     DASPGK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162299"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VEC56_05075"
     CDS             complement(<1..585)
                     /locus_tag="VEC56_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV13558.1"
                     /translation="MSSRYTTLGLEKDGPVARLTLNRPEVRNAFDDVMIGELADVAAS
                     LSSDESVRVVVLTGAGEVFSAGADLNWMKKVAQYSFEENVKDALTFARMIESLYRMPK
                     ATIARINGACLGGGVGLVSACDVAVAVREAKFALREILLGIAPAAISPYVLRKMGESH
                     AHDYFLTGRTFDAERAREIGLINEVVDGELLDAET"
     gene            complement(594..2189)
                     /locus_tag="VEC56_05080"
     CDS             complement(594..2189)
                     /locus_tag="VEC56_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXV13559.1"
                     /translation="MYRIESKVQTSSSVFQENAAFQREASARLRERLDAVKQGGPPSA
                     VEKHKARGKLTARERIAKLLDRDTPFLEFSALAANGMYDDQAPSAGVITGAGVIHGRE
                     VVVVANDATVKGGTYFPMTVLKHVRAQQIAIENRLPCVYLVDSGGVFLPLQDGVFPDK
                     DHFGRIFYNQAVMSAAGIPQICAVMGSCTAGGAYVPAMSDENVIVRGQGTIFIGGPPL
                     VKAATGVDVTPEELGGADVHTRISGVSDHFADDDDHAIQILRNSVEGFGRVEKTKLEI
                     HEPEDPYYDPQELYGIVPKDLSKPFDSREVIARIVDGSRFHEFKARYGTTLVTGFAHI
                     MGYPVGIVANNGILFSESARKGAHFVSMCAVRRIPLLFLQNITGFIVGKEFEHGGIAR
                     DGAKMVHAVATAQVPKFTVIVGGSYGAGNYAMCGRGYSPRLLWMWPSAKISVMGGQQA
                     ADVLWTVKQSEGKQPSAGEEADFKKPILDKYEREGNAYYSTARLWDDGVIDPVDTRTA
                     VALGISLSLNAYIPKHRFGVFRM"
     gene            2313..2945
                     /locus_tag="VEC56_05085"
     CDS             2313..2945
                     /locus_tag="VEC56_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV13560.1"
                     /translation="MRILVIGATGGTGREIVKQALERGHHVVALARKPSHVKIEDPRL
                     RVAEGDVMGPDSLDAAMANVDAVVCALGHKRWLGPSRILSAGTRNVVRAMEKHGVKRL
                     VVETALGVGDSAGRLGLYYTLFTIPFILPFYWYDKGRQERVVRDSSLDWVIVRPAQLT
                     NGRKRGTYRHGPRVGNYIWSVSISRADVADFMLNQLGETPYLRQAPGVCY"
     gene            complement(2939..3823)
                     /locus_tag="VEC56_05090"
     CDS             complement(2939..3823)
                     /locus_tag="VEC56_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13561.1"
                     /translation="MTKAQTLIAFALVLAAPARGQDAFESHWRDGQAELDGYRLVVSR
                     YGQDRSGTAVMIFVTEPFSESKRVKDDRPPHNSRDTIDVLKLNLVRDFQTGVYDYNTM
                     ASVFVRASDFEPVKASFSSAEWCGHVYAEMIFRPKQIRGMYASYFEDESGPIVLERPK
                     GGITEDQLFIALRGLRAEFLPAGEQRTVPYLPGLFFTRLSHQPLRWTQAVIKREAVQQ
                     SVTVPAGTFDAMLYDVRIGDGRTGQFFIEAAYPHRIVKWSLPPDVGGELTGSTRLPYW
                     KLNSEGDESYLKEIGLGQ"
     gene            complement(3820..4209)
                     /locus_tag="VEC56_05095"
     CDS             complement(3820..4209)
                     /locus_tag="VEC56_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HXV13562.1"
                     /translation="MPPHKDQDFLEFVLDQLSALKSVTSRRMFGGIGLYRAETFFAII
                     AEGRLYFVTDEATRAAYVDRGMGPFQYAPGKVIQTYYEVPVDVLEDDGALNDWARAAL
                     DAQTRRKTKPARSRTKAKTRTTARKRS"
     gene            4282..5064
                     /locus_tag="VEC56_05100"
     CDS             4282..5064
                     /locus_tag="VEC56_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13563.1"
                     /translation="MRPRTTGLGIAAALVLAAGCSKNQSTAPALKGPQIIHEMIAAHG
                     GMEEWRNAASVSFTDQWGESSPRMGFVVEQSSRRAYFDIPGTATAGGWDGQRAWSMNW
                     PTPTPVRFLALLNYYFINLPWLTQDPGVILGEPGTGRLHDDPTEYVTVMMTFEPGTGD
                     TPDDYYRLYIHPETKMLRACDYIVTYRALLDAGETSTPEHCLVYDQMTPVEGVTVPSH
                     FTIYEGDRVYAECSISGYSLAKAFDESRMTMPEGAVVDTSTP"
     gene            5228..6712
                     /gene="sufB"
                     /locus_tag="VEC56_05105"
     CDS             5228..6712
                     /gene="sufB"
                     /locus_tag="VEC56_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXV13564.1"
                     /translation="MAGKAESIRPHDDSIDPIQERISKEYEWGFVTEVEEDKPPKGLS
                     EDVVRAISARKKEPEWLLEWRLRAYRAWIKMAAEEPTWANIKHPPIDFQDIHYYSAPK
                     KAGDQPKSLDEVDPEIRRTFDKLGIPLVEQMRIAGVAVDAVFDSVSVATTFKEKLGSL
                     GIVFCSFSEAVQTHPELVKKALGTVVPYNDNFYAALNSAVFSDGSFVYVPAGVRCPME
                     LSTYFRINARETGQFERTLIVAEKGAYVSYLEGCTAPIRDENQLHAAVVELVAEEDAT
                     VKYSTVQNWYPGDKQGKGGIYNFVTKRGECRGARSKISWTQVETGSAITWKYPSCVLT
                     GDDSTGEFYSVALTNNYQQADTGTKMIHRGKNTRSTIISKGISAGHGQNSYRGLVRML
                     PEASNARNFTQCDSMLIGDKCGAHTFPYIEVGNSTSRAEHEATTSKIGEDQLFYCRAR
                     GISNEDAVHMIVNGFCREVFAELPMEFAVEAQRLLAVSLEGSVG"
     gene            6729..7469
                     /gene="sufC"
                     /locus_tag="VEC56_05110"
     CDS             6729..7469
                     /gene="sufC"
                     /locus_tag="VEC56_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXV13565.1"
                     /translation="MLEIKNLHARVDEKEILKGVNLTVKAGEVHAIMGPNGSGKSTLA
                     QVLAGRDTFEVTEGDILIGGESVLELDPEERAAKGLFLAFQYPVEIPGVRNNYFLKTA
                     LNSLRKSRGEKELDAMEFLKLVKEKMKILKIDESFMNRSVNEGFSGGEKKRNEILQMA
                     ILDPMLAVMDETDSGLDIDALRVVANGVNALRSPQRAMVVITHYQRLLNYIVPDVVHV
                     FVDGRVIKTGGKELALELEKEGYAEVTG"
     gene            7469..>7932
                     /locus_tag="VEC56_05115"
     CDS             7469..>7932
                     /locus_tag="VEC56_05115"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXV13566.1"
                     /translation="MPGVAAYESAFAKLEPALKASGPAWLHDLRREGLERFKSLGFPS
                     LKEEEWRFTRTRPIAELDFVPAAAAPALADAVITSRTFDDSSCRRLVLVNGALAPKLA
                     QGPEWPKGVRAESLAEAMRRDGERIRELLARGARAENQRFAALNTAFLNDGVL"
BASE COUNT         1451 a         2583 c         2553 g         1345 t
ORIGIN      
        1 ggtctccgcg tccagcagct cgccgtccac gacttcgttg atgagcccga tctcgcgcgc
       61 gcgctccgcg tcgaaggtac gcccggtgag aaagtaatcg tgcgcgtgcg actcgcccat
      121 cttgcgcagc acgtagggcg agatggccgc gggggcgatg cccaggagaa tctcgcgcag
      181 cgcgaacttg gcctcgcgca ccgcgaccgc gacgtcgcac gcgctcacca gtcccacccc
      241 gccgcccagg cacgcgccgt tgatgcgcgc gatggtcgcc ttcggcatgc gatagagcga
      301 ctctatcatg cgcgcgaagg tgagcgcgtc cttgacgttc tcctcgaacg agtactgcgc
      361 caccttcttc atccagttca agtcggcgcc ggcggagaac acctcgcccg cgcccgtcaa
      421 gaccaccacg cgcaccgatt cgtcgctcga gagcgatgcg gcgacgtcgg cgagctcgcc
      481 gatcatcacg tcgtcgaagg cgtttcgcac ctcggggcgg ttgagcgtca gacgcgcgac
      541 cgggccgtcc ttctccaatc cgagcgtggt gtagcgggaa gacatgcgag ctcctacatg
      601 cgaaaaacgc cgaagcgatg cttggggatg tacgcgttga gcgacaagct gatgccgagg
      661 gcgaccgcgg tgcgcgtgtc gacggggtcg atgacgccgt catcccacag gcgcgcggtc
      721 gagtagtagg cattgccctc gcgctcgtac ttgtcgagga tgggcttctt gaaatctgct
      781 tcttcgcccg cggagggctg cttcccttcc gactgcttga ccgtccacag cacgtcagcc
      841 gcctgctggc cgcccatcac ggagatcttc gcggagggcc acatccacaa taggcgcggc
      901 gagtatccgc gcccgcacat cgcgtaattg cccgcgccgt acgacccccc gacgatgacg
      961 gtgaacttgg gcacctgggc ggtcgcgacc gcgtgcacca tcttggcccc gtcgcgcgcg
     1021 ataccgccgt gctcgaactc cttgcccacg atgaagccgg tgatgttctg caggaacagg
     1081 agcgggatgc gccgcaccgc gcacatggag acgaagtgcg cgcccttgcg cgcgctctcc
     1141 gagaagagaa tgccgttgtt ggcgacgatc ccgaccgggt agcccatgat gtgcgcgaag
     1201 ccggtgacca gtgtggtgcc gtagcgcgcc ttgaattcgt ggaagcgcga tccgtcgacg
     1261 atgcgcgcga tcacttcgcg cgagtcgaag ggcttggaga gatccttcgg cacgatgccg
     1321 tagagctcct gtgggtcgta atacgggtcc tcgggttcgt ggatttcgag tttcgtcttc
     1381 tcgacccgcc cgaagccctc cacgctgttg cgcaggatct ggatcgcgtg gtcgtcgtcg
     1441 tccgcgaaat gatccgacac gccggagatg cgcgtgtgca cgtcggcccc gcccaattcc
     1501 tcgggtgtca cgtcgacgcc ggtcgccgcc ttgaccagcg gcggcccgcc gatgaagatg
     1561 gtgccctgcc cgcgcacgat cacattttcg tcgctcatgg cgggaacgta ggcgccgccc
     1621 gccgtgcacg accccatcac cgcgcagatt tgcggaatgc cggcggcact catgacggcc
     1681 tggttgtaaa agatgcgccc gaaatgatcc ttgtcgggaa acacgccgtc ttggaggggg
     1741 aggaacacgc cgccggagtc gaccaggtac acgcacggca gtcgattctc gatcgcgatc
     1801 tgctgcgcgc gcacgtgctt caagaccgtc atgggaaagt aggtgccgcc cttgacggtg
     1861 gcgtcgttgg cgaccaccac cacctcgcgc ccatgaatca cgcccgcgcc cgtgatcaca
     1921 ccggccgagg gcgcctggtc gtcgtacatg ccgttggccg ccagcgccga gaactcgagg
     1981 aagggcgtgt cccgatcgag cagcttcgcg atgcgctcgc gcgcggtgag cttgccgcgc
     2041 gccttgtgct tctcgaccgc actcggcggc ccgccctgct tgaccgcgtc caagcgctcg
     2101 cgcaggcgcg cggacgcctc gcgctggaag gcggcgtttt cttggaagac ggaggaggac
     2161 gtctggactt tggattcgat tcggtacatc ggtaatggcc tgtgtgacag tgagttgcac
     2221 tccccgagag gccggggcaa cgggcctagg atagaacaaa accccttccc gccgtaagcg
     2281 caatttgcca ctcgatctgc tagagtcgcg cgatgcgaat cctcgtcatc ggcgcgaccg
     2341 gcggtaccgg tcgcgagatc gtgaagcaag cgctcgagcg cggccaccac gtcgtcgcgc
     2401 ttgcgcgcaa gccatcccac gtcaagatcg aggacccgcg actgcgcgtc gcggagggcg
     2461 acgtgatggg gccggattcg ctcgacgcgg cgatggcgaa cgtcgacgcg gtcgtgtgcg
     2521 cgctcggcca caaacggtgg ctgggcccgt cgagaatcct ctcggctgga acgcgcaacg
     2581 tcgtccgagc gatggagaaa cacggcgtca aacgactcgt ggtcgagacc gcgctcggcg
     2641 tgggcgacag cgcggggcgc ctcggcctct actacacgct cttcaccatc ccgttcattt
     2701 tgccgttcta ctggtacgat aagggacggc aggaacgcgt cgtgcgggat agttcgctcg
     2761 actgggtgat cgtgcgaccg gctcaactga ccaacggtcg caagcgcggg acctaccggc
     2821 acgggccccg cgtcggaaac tacatttggt cggtgagcat ctcgcgagcg gacgtggccg
     2881 acttcatgtt gaaccagctg ggcgaaacgc cgtatctgcg acaggcaccc ggcgtctgct
     2941 attgaccgag cccgatctcc ttcagatacg attcgtcgcc ttctgaattg agcttccagt
     3001 agggcaagcg cgtcgatccg gtgagttcgc cgcccacgtc gggcggcagc gaccacttca
     3061 cgatgcggtg cgggtaagca gcctcgatga agaactgccc cgttcgaccg tccccgattc
     3121 gcacgtcgta gagcatcgcg tcgaacgtgc ccgcggggac cgtcaccgat tgttgcaccg
     3181 cttcgcgttt gatgacggcc tgcgtccagc gcagcggctg gtgcgacaag cgcgtgaaga
     3241 acagtcccgg tagatacggc accgtccgct gctcgcccgc ggggaggaac tcggctcgca
     3301 gcccgcgcaa cgcgatgaac agctggtcct cggtaatccc gcccttcggt cgctcgagca
     3361 cgatcggtcc cgattcgtcc tcgaagtagc tcgcgtacat tccgcgaatc tgtttggggc
     3421 ggaaaatcat ttccgcgtag acgtgcccgc accattcggc gctcgaaaac gacgccttca
     3481 cgggctcgaa gtccgacgcg cgcacgaaca cggacgccat ggtgttgtag tcgtagacgc
     3541 cggtttggaa gtcgcgcact aggttgagct tcagcacatc gatggtgtcg cgcgagttgt
     3601 ggggcggacg atcgtccttc acgcgtttcg attcgctgaa gggctcggtg acgaagatca
     3661 tcaccgcggt gcccgaacgg tcctggccgt agcgagaaac gaccaggcga tatccgtcga
     3721 gctcggcctg gccgtcccgc caatgggact cgaaggcgtc ttggccgcgc gcgggggccg
     3781 ccagcaccag ggcaaacgcg atgagggtct gggctttcgt catgatcgct ttctcgccgt
     3841 cgttcgcgtt ttcgctttcg tgcgcgatcg tgcgggcttc gtcttgcgtc gcgtttgcgc
     3901 atcgagcgcc gcgcgcgccc agtcgttcaa ggcgccgtcg tcctcgagca cgtcgacggg
     3961 gacttcatag tacgtttgaa tgaccttgcc gggcgcgtac tgaaagggcc ccatgccgcg
     4021 gtcgacgtag gccgcgcgcg tggcctcgtc ggtgacgaag tacaggcgtc cctcggcgat
     4081 gatggcgaag aacgtctcgg cccgatacaa gccgatcccg ccgaacatcc ggcgcgaggt
     4141 gaccgacttg agcgcggaga gttgatcgag cacgaactcc aggaagtctt ggtccttgtg
     4201 cggcggcatg ggaggaatgt accgcggggg atgaaccccg acaagcggcg tgctagactg
     4261 gccgcgtcga aaggagcctc catgcgtcca cgcacgaccg gtctgggaat cgccgcggcc
     4321 cttgtcctcg ccgccgggtg ttcgaagaac caatcgaccg caccagccct caaaggcccg
     4381 caaatcattc acgagatgat cgcggcgcac ggcggtatgg aagaatggcg aaacgccgcc
     4441 agcgtttcgt tcaccgacca atggggagag tcgtcgccgc gcatgggctt cgtggtcgag
     4501 cagtcgagtc gacgagccta cttcgacatt cccggaaccg ccaccgcggg cgggtgggac
     4561 ggccagaggg cgtggagcat gaactggccc acaccgacgc cggttcgctt ccttgcgctg
     4621 ctcaactact actttatcaa tctgccgtgg ctgacccagg accccggtgt gatcctcggc
     4681 gaacccggca ccggccggct gcacgacgac ccgaccgagt atgtgaccgt catgatgacg
     4741 ttcgaacccg ggaccggcga tacgcccgac gattattacc gcctctacat ccatcccgag
     4801 accaagatgc tgcgtgcctg cgactacatc gtgacctatc gggcgctgct ggacgcgggc
     4861 gagacctcga cacccgaaca ctgcctggtc tacgaccaga tgacccccgt cgaaggtgtc
     4921 acggtgccct cccacttcac catctacgag ggcgatcggg tctacgccga gtgctcgatc
     4981 agcgggtact ccctcgcgaa ggccttcgac gagtcccgga tgacgatgcc cgagggggcg
     5041 gtcgtagaca cctcgacccc gtagcgcgcg tgcgtatccc ggcccccaac agccgcttag
     5101 cggctcggcc aggtagtcgg aatcgtgggt tgattccgca tggtgaattg tttatcctac
     5161 aaagcggacc aaattagtgc gattaaatag attagcggcg cccagcgggc gcccggagga
     5221 agaccccatg gcgggcaaag ccgaatccat tcgaccccac gacgactcga tcgaccccat
     5281 ccaggagcgg atctccaaag agtacgagtg gggcttcgtc accgaggtcg aagaagacaa
     5341 gccacccaag ggattgagcg aggacgtcgt ccgcgccatc tccgcgcgca agaaggagcc
     5401 cgagtggctg ctcgagtggc gcctgcgcgc gtaccgcgcg tggatcaaga tggcggccga
     5461 agagccgacc tgggcgaaca tcaagcaccc gcccatcgac ttccaggaca tccactacta
     5521 ctcggccccc aagaaggccg gcgaccagcc caagagcctg gacgaagtcg accccgagat
     5581 ccggcgcacc ttcgacaagc tcggcattcc gctggtcgag caaatgcgca tcgccggggt
     5641 ggcggtcgac gccgtgttcg actcggtctc ggtcgcgacc accttcaagg agaagctcgg
     5701 ctcgctcggg atcgtgttct gctcgttctc cgaagcagtg cagacccatc ccgagctggt
     5761 gaagaaggcg ctcggcaccg tcgttcctta taacgacaac ttctacgccg cgctcaactc
     5821 cgcggtattc agcgacggct cttttgtata cgtcccggcc ggcgtgcgct gtccgatgga
     5881 gctctcgacc tatttccgca tcaacgcgcg cgagacgggc cagttcgagc gcacgctgat
     5941 cgtggccgaa aaaggcgcgt acgtctcgta cctggaaggt tgcacggcgc ccattcgcga
     6001 cgagaaccag ctgcacgcgg cggtggtcga attggtggcc gaggaagacg cgaccgtcaa
     6061 gtactcgacg gtgcagaact ggtaccccgg cgacaagcag ggcaagggcg gcatctataa
     6121 tttcgtcacc aagcgcggcg agtgccgcgg cgctcgctcg aaaatctcgt ggacccaggt
     6181 cgagacgggc tccgcgatca cgtggaagta cccgagctgc gtgctgaccg gcgacgattc
     6241 gaccggcgaa ttctattcgg tggcgctgac caacaactat cagcaggccg acaccggcac
     6301 caagatgatc caccgcggca agaacacgcg cagcaccatc atctcgaagg ggatctcggc
     6361 cggccacggg cagaacagct accgcgggct ggtgcggatg ctcccggagg cgtccaacgc
     6421 gcgcaatttc acgcagtgcg actcgatgct gatcggcgac aagtgcggtg cgcacacgtt
     6481 cccgtatatc gaagtcggca actcgacctc gcgcgccgag cacgaggcca ctacctccaa
     6541 gatcggcgag gaccagctgt tctactgccg cgcgcgcggt atctccaacg aggatgccgt
     6601 gcacatgatc gtcaacggat tctgccgcga agtcttcgcc gagctgccga tggagttcgc
     6661 ggtcgaagcc cagcgcttgc tcgcggtcag cctggaaggg tcggtcggtt agtcacatgg
     6721 gagagaaaat gctcgaaatc aagaacctgc acgcgcgcgt agacgagaag gaaatcctca
     6781 aaggcgtcaa cctcaccgtc aaggcgggcg aggtgcacgc gatcatgggc cccaacggat
     6841 cgggcaagag cacgctcgcg caggtgctgg cggggcgcga cacattcgag gtgaccgagg
     6901 gcgacatcct gatcggcggc gagagcgtcc tcgagctcga ccccgaggaa cgcgccgcca
     6961 agggtctctt cctcgccttc cagtacccgg tcgagattcc gggcgtgcgc aacaactact
     7021 tcctgaagac ggcgctcaat tccctgcgca agtcgcgcgg cgagaaagag ctcgacgcga
     7081 tggagttcct gaagctcgtc aaagagaaga tgaagatctt gaagatcgac gagagcttca
     7141 tgaatcgctc cgtcaatgag ggcttctcgg gcggcgagaa gaagcgcaac gagatcctgc
     7201 agatggccat cttggacccc atgctcgcgg tcatggacga gaccgactcg ggcctcgaca
     7261 tcgacgccct gcgcgtcgtc gccaacggcg tcaacgccct gcgcagcccc cagcgcgcga
     7321 tggtcgtcat cacgcactac cagcgtttgt tgaactacat cgtgcccgac gtggtacacg
     7381 tcttcgtcga cggccgcgtc atcaagaccg gcggcaagga gctggcgctc gagctcgaga
     7441 aggaaggcta cgcggaggtg acgggctagt gccgggtgtc gccgcttacg agagcgcgtt
     7501 cgccaaactc gagcccgcct tgaaggcgag cggccccgcg tggctgcacg acctgcggcg
     7561 cgagggcctc gaacgcttca agtcgctcgg tttcccttcg ctcaaggaag aggagtggcg
     7621 ctttacgcgc acgcgtccga tcgccgaact cgacttcgtc cccgcggccg ccgcgcccgc
     7681 gctggccgac gctgttatca cctcgcggac cttcgacgac tcgagctgcc gccgcttggt
     7741 gctggtcaac ggagcgctcg cgccgaagct cgcgcagggt ccggagtggc ccaagggggt
     7801 gcgcgcggag agcctcgccg aggcgatgcg ccgcgacggt gagcgcattc gcgagctact
     7861 ggcgcgcggc gcgcgagccg aaaatcagcg cttcgcggcc ctcaacaccg cgttcctgaa
     7921 cgacggcgtt ct
//
LOCUS       DASPGK010000178         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_981941, whole genome shotgun sequence.
ACCESSION   DASPGK010000178 DASPGK010000000
VERSION     DASPGK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981941"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..835)
                     /locus_tag="VEC56_05120"
     CDS             complement(116..835)
                     /locus_tag="VEC56_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXV13567.1"
                     /translation="MPRRKTGIVVSCEHAGNRVPTAYRSLFRGHAGALKSHRGWDPGA
                     LDLARAVAFACHAPLLANTTTRLLVECNRSIGSAELFSEFTRDLDTSEHMRLLVTHYL
                     PHRRAVDIAVRRALRRHERVVHIGVHTFTPVLGGKRRSADLGLLYDPSRRFEVEVAGA
                     LATSLRAFGPTLRVRRNYPYRGWTDGLTTTLRRRFSAQGYAGIEIEVNHALMRKPVVW
                     KQVKRALADAARAGIQLSQDF"
     gene            complement(822..>2026)
                     /locus_tag="VEC56_05125"
     CDS             complement(822..>2026)
                     /locus_tag="VEC56_05125"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HXV13568.1"
                     /translation="SLDVLPVVDKLIVQVAGDYEAEVERGPLAWSNELVNHVIELKTN
                     GPAPALFRLDEVFAAHVDEIAALLKPMGGRLMPTAAHPWMDPTRDTVLWPHEMGEVYR
                     AFDRIFGCKGHGWSNLQCVHLNLPFQGDDEFARLHAAIRLVLPLVPALAASSPILDAR
                     LTGLMDSRMHYYRENQKRIPSIAGSVIPEPVYSKREYEERILAPMYVDIAPHDQEGIL
                     QEEWLNARGAIARFDRDAIEIRIIDIQECPTADLAVSHAIACVLRALVKERWQALERQ
                     KRFATEALDAILLATTRDAEATVLEDREFLDAFGVRAKRASAGEVWEHLVETCVAGDA
                     EFTAGWASVMGTILGEGTLARRIVRAVAGDNPSVAARLSLPRERLYDVYAELCDCLKE
                     NRTFHAEA"
BASE COUNT          320 a          678 c          696 g          332 t
ORIGIN      
        1 cgaaggacgt gacccgcttc cgtttctacg gtcgtgcgct ggctcgggga tatggcgcgc
       61 aagcgaccgt ctgcccattt cgacccacga cggcgccgac aatcacacga agccgtcaga
      121 aatcttgaga aagttggatg ccggcccgcg ccgcgtccgc cagcgcgcgt ttaacctgtt
      181 tccacacaac cggtttcctc atgagggcgt ggttgacctc gatctcgatc ccggcgtagc
      241 cctgtgcgga gaatcggcga cgaagcgtgg tcgtgaggcc gtcggtccac cctcgatagg
      301 ggtagttgcg cctgacccgg agggtcggcc cgaaggcgcg caggctcgtc gcgagcgcgc
      361 cggcgacctc gacctcgaag cgccggctcg ggtcgtagag caggccgagg tccgcgctgc
      421 gacgcttccc gcccagcacg ggcgtgaagg tgtgcacgcc gatatgcacg acgcgttcgt
      481 gccgccgaag cgctcgccgc accgcgatgt cgaccgcgcg ccgatgggga aggtagtggg
      541 tcacgagcag gcgcatgtgc tccgaggtgt cgaggtcgcg cgtgaactcg gagaagagct
      601 ccgccgatcc gatcgagcgg ttgcactcga ccaaaaggcg cgtggtcgtg ttggcgagca
      661 acggagcgtg gcacgcgaac gcgacggcgc gtgcgaggtc gagtgctccc gggtcccacc
      721 cgcggtgcga tttcagcgca ccggcgtggc cccggaacag cgaccggtac gcggtcggca
      781 cgcgattgcc cgcgtgctcg cacgatacga cgattcccgt cttacgcctc ggcatggaac
      841 gtccggttct ccttcaggca gtcgcacaac tcggcgtaca catcgtacag ccgctcgcgc
      901 gggagcgata gtcgcgccgc gacgctcggg ttatcgcccg ccaccgcgcg cacgatgcgt
      961 cgcgcgagcg tcccctcgcc caggatcgtg cccatgacgg acgcccagcc ggcggtgaac
     1021 tcggcgtcgc cggccacaca cgtctcgacc aggtgctccc agacctctcc cgcgctcgcc
     1081 cgcttcgcgc gcacgccgaa ggcgtccagg aactcgcggt cctcgagcac ggtcgcttcc
     1141 gcgtcgcgcg tcgtcgccaa gaggatcgcg tcaagcgcct cggtggcgaa gcgtttctgg
     1201 cgttcgagcg cttgccagcg ctccttgacg agcgccctca gcacgcaggc gatcgcgtgc
     1261 gagacggcca ggtcggcggt gggacattct tggatgtcga tgatccgaat ctcgatcgcg
     1321 tcccggtcga agcgcgcgat ggccccgcgt gcgttgagcc attcctcctg caggatgccc
     1381 tcctggtcgt gcggcgcgat atcgacgtac atgggcgcca aaatgcgctc ctcgtactcg
     1441 cgcttcgagt agacgggctc cggaatcacg ctgcccgcga tcgacggaat gcgcttctgg
     1501 ttctcgcggt agtagtgcat gcggctgtcc atcaagccgg tgaggcgcgc gtccagaatg
     1561 ggcgagctgg cggcgagcgc ggggaccagc ggtaggacca ggcggatggc cgcgtgcagg
     1621 cgcgcaaact cgtcgtcgcc ttggaagggg aggttcaagt gcacgcactg gaggttcgac
     1681 caaccgtgtc ccttgcaccc gaagatgcgg tcaaaggcgc ggtagacctc tcccatttcg
     1741 tgcggccaca gcacggtgtc gcgcgtgggg tccatccacg ggtgcgcggc cgtcggcatg
     1801 aggcggccgc ccatgggctt cagcagcgcc gcgatctcgt cgacgtgggc cgcgaagacc
     1861 tcgtcgaggc gaaagagcgc gggcgcgggc ccgttggtct tgagctcgat cacgtggttg
     1921 acgagttcat tcgaccacgc gagcggtccg cgctcgactt ccgcctcgta gtcgcccgcg
     1981 acctggacta tcagcttgtc gaccacggga agcacgtcga gcgaat
//
LOCUS       DASPGK010000179         7762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1099949, whole genome shotgun sequence.
ACCESSION   DASPGK010000179 DASPGK010000000
VERSION     DASPGK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099949"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /gene="ftsH"
                     /locus_tag="VEC56_05130"
     CDS             <1..1334
                     /gene="ftsH"
                     /locus_tag="VEC56_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXV13569.1"
                     /translation="GTGKTLLARAVAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFE
                     TGKKHAPCILFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVILVAA
                     TNRPDVLDPALMRPGRFDRRIVVDLPDVRGREGVLKVHSRKTPLAEDVDLAVLARATP
                     GMSGADLANLVNEASLLAARRNANKVMMDDLEEAKDKIYLGPERKSRVVREETRRLTA
                     YHEAGHAVVGSALEYSDPVHKVTIIPRGNAGGLTFFLPEDDRSEYSKAWCEDTITQAL
                     GGRVAEEMVLNRMGSGAQSDIQMVSKLVRKMVMQWGMSPKIGPIAFGERDEQVFLGRD
                     ITQTPEYSEKTAQEIDDEIKRIIDESYDRAKKILEDRIDILHRMANALLERETLDAAE
                     IKVILEGGTLEPMPPRLSVKPAEEAATERAVEPERKKIPGIPPIAEPGPTT"
     gene            1365..2255
                     /gene="folP"
                     /locus_tag="VEC56_05135"
     CDS             1365..2255
                     /gene="folP"
                     /locus_tag="VEC56_05135"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXV13570.1"
                     /translation="MLPTRPGRQSIRFKDRTLSFGRTPIVMGILNVTPDSFSDGGRFV
                     DVRTAVAHAHAMVEAGAALVDVGGESTRPGSEPVPASLEIDRVVPVIDALARGGFDHG
                     PLPAPVSIDTRKPKVADAALRAGATMVNDVTAGRDPAMIDVLRWREDVPVVLMHMLGE
                     PKTMQAEPRYDDVLREVGRYLDERARALIEAGVARERIVIDPGIGFGKRFEDNIALLK
                     GLETLRKLGYPVMVGASRKAFIGTLLAGDAGAPAAGDRVAGSLAVAAHCYHAGVEMIR
                     VHDVRETVQLLRVLDAVHSD"
     gene            2257..3033
                     /gene="cdaA"
                     /locus_tag="VEC56_05140"
     CDS             2257..3033
                     /gene="cdaA"
                     /locus_tag="VEC56_05140"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXV13571.1"
                     /translation="MPHIPIELVVDVIDIAIVSFLLYHLFALMRGTRAVHMFFGLIVL
                     FLLSVIAQWMNLIAVNWLISSLRTAWVIAFVIIFQPELRRALSMLGQNRLLSRFVTMR
                     QSHVIPEVVKAVQEMAEKKTGALIVLEKDMGLKNYVETGTTVDARVSAELIESIFTRP
                     SPLHDGAIVIQNDRVVAAGCTLPLSQDQRAAQSMGMRHRAALGLSEETDALVVVISEE
                     SGSIAYAEDGKLYRKIDLTTLKNELFRSFGVAGEERGAAG"
     gene            3199..4371
                     /locus_tag="VEC56_05145"
     CDS             3199..4371
                     /locus_tag="VEC56_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13572.1"
                     /translation="MLPATVIVLLASSGAKAQLPTVVTGGDVFVYATFVSPPGTISFD
                     AFDNMFVGNFNNPGPPNGPAPVWVVDAEDSTVSTCATIDDPDAVYIDRNGLLSLSGTL
                     LVGGLISPTTTGQIRGVTNCGGSSLLASGGCVHNVRMFAEDALGNLYVANATSRTICR
                     WNGATWAQFLPAFPLAGPIEPAIAIDGTDVYVSAAGVVKLYDTSGAVVDPAFAVGEVV
                     GIGPFATAFEGVIVSRADSLELVAVDPDTKRERPILTGPGSPGVVAFDSAGDLYVAQD
                     YANRVLHVTQPIPTHVPGVISTRTCTLYQNVPNPFNPTTTIQFEIPRSASTQLDVFDV
                     SGRHVRSLLRGMIGKGPHAVTWDGTNHRGELVGSGVYFYRLEVGGERLTRKMVLLK"
     gene            4833..5381
                     /locus_tag="VEC56_05150"
     CDS             4833..5381
                     /locus_tag="VEC56_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXV13573.1"
                     /translation="MSIDDLIRAHNAGEAAATSHLLELLYAELRRIASRLMSRERPGH
                     TLQTTALVSEAYLRLVGGGSTEISDRKHFLCLAATTMRNILFDHARERDAIKRGGGAA
                     HVTLDHSWLAAPEESMDTQALKEAIEALSKEYPRHVRVIDLRYFVGFSVEETADLLQV
                     CEKTIKNDTQFAKAWLRRRLVA"
     gene            5394..>7762
                     /locus_tag="VEC56_05155"
     CDS             5394..>7762
                     /locus_tag="VEC56_05155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV13574.1"
                     /translation="MDAERSARVSDLFDHAKELPPAERDAFLARECAGDTELLHEVRS
                     LLDHHVDEPESRFIGWNANHAPAVDDEIKPGDVVQRFRIARTIGEGGMGIVYLAEQSS
                     PVRRKVALKIVRVGMDTKELLARFEAERQALAMMDHPAIARIYDAGATERGRPYFAME
                     YVDGVPITEYCDQRRLDVTSRLRLFADVCNAIHHAHQRGIIHRDIKPTNVLVTEYEGK
                     PAPKVIDFGIARATDQSLAAETMHTRRDVMLGTPAYMSPEQADPMEPPDTRTDVYSLG
                     ALLYQLLTGRPPLEFGERQTVESIRRTIIDTDPPRPSARLTSLGEKAAAVAAERRTDA
                     KHLLSLLRNDVDWIVMRALEKDRARRYGSASELAADIQRFLDREPVVARPPTVGYRVR
                     KYARRHRYLMAGATAVFVVLVAGLTATIWQAREARQRTRELEAIAARLVSHFYELSDY
                     PGAMAAKQTLAELATQTLDDLTKRGGDDPSVRRQLASAYEGLGQLLGDNYSASLGQSA
                     AAVVARKKALDIREELHRANPADFGLANEYLKSYAAWGMLALFGYLDEVGIPGVAWHP
                     EVIPTAQQRRDRKMILSLSDDVASERAWFLGRTDSMVFYIERARARREALLAEEPGNL
                     EYQRLLADTYDRLAYLTRDVDEAERLARRAVEFCENYYRAHPFSWKASMDVAYTRRNL
                     GYARWRKGDLAGGLEEQRLGLEARRRLASADSMNVRGTFLLSEGYSLVGQAFLRMGRP
                     DSSIHYSRQALGLLYELAKSPNPPDWTGNELAFVHTYLANAHQDRNDAES"
BASE COUNT         1441 a         2556 c         2465 g         1300 t
ORIGIN      
        1 cgggtaccgg caagacgctc ctcgcgcgcg cggtcgcggg cgaagcgaac gtgcccttct
       61 tctcgatgag cggctcggac ttcgtggaga tgttcgtggg cgtgggtgcc tcgcgcgtgc
      121 gcgacctctt cgagaccggc aagaagcacg cgccctgcat cctcttcatc gacgagatcg
      181 acgcagtcgg gcgccaccgc ggcgccggac tgggcggcgg ccacgacgag cgcgagcaga
      241 cgctcaatca gttgttggtc gagatggacg gcttcgaatc gaacgacggc gtgattctgg
      301 tggctgccac caaccgtccc gacgtgctcg acccggcgct catgcgcccg ggccgtttcg
      361 accgccgcat cgtggtcgac ctgcccgacg tgcgcggccg cgaaggtgtt ctcaaggtac
      421 attcgcgcaa gaccccgctc gcggaggacg tcgatctggc cgtcctcgcg cgcgcgacac
      481 ccggcatgag cggcgccgac ctcgcgaacc tggtcaacga ggcgtctctc ctcgcggcgc
      541 gccgcaacgc caacaaggtg atgatggacg acctcgagga ggccaaggac aagatctacc
      601 tgggccccga gcgaaagagt cgcgtggtgc gggaagagac ccggcgcctg accgcgtacc
      661 acgaagccgg ccacgcggtg gtgggatcgg cgctcgagta ttccgacccg gtgcacaagg
      721 tgacgatcat cccgcgcgga aacgcgggtg ggctgacctt cttcctcccc gaagacgatc
      781 gctccgagta ctcgaaggcg tggtgtgagg acaccattac gcaagccctc ggcggccgcg
      841 tggccgagga gatggtgctg aaccgcatgg gcagcggcgc gcagtccgac atccagatgg
      901 tgtcgaagct ggtgcgcaag atggtcatgc agtggggcat gagtccgaag atcggaccca
      961 tcgcgttcgg cgagcgcgac gagcaggtgt tcctcggccg cgacatcacg cagacgcccg
     1021 agtacagcga gaagaccgcg caggagatcg acgacgagat caagcgcatc atcgacgagt
     1081 cgtacgaccg cgcgaagaag atcctcgaag atcgcatcga catcctgcat cgcatggcca
     1141 acgcgttgct cgagcgcgag acgctcgacg cggccgagat caaggtcatc ctggaaggtg
     1201 gtacgttgga accgatgccg ccgcgtttgt ccgtgaagcc tgcggaagag gcggccaccg
     1261 aacgcgcggt cgagcccgag cgcaagaaga tccccgggat tccgcccatc gccgaacccg
     1321 ggccgacgac gtagccggcg ccgcgtcagc ggcgcctttt ttccatgctc ccaacccgac
     1381 ccggccgtca atcgattcgg ttcaaggatc gaacgctgtc gtttggtcga actcccatcg
     1441 tcatgggcat cctcaacgtc acgcccgatt ccttctcgga cggcgggcgc ttcgtcgatg
     1501 tgcgcacggc ggtggcccac gcgcacgcca tggtggaagc cggagccgcc ctcgtcgacg
     1561 tgggcggcga gtcgacgcga cccggctccg agccggtgcc cgcctcgctc gaaatcgatc
     1621 gcgtggtgcc ggtgatcgac gcgttggcgc gcgggggctt cgaccacggt ccgctgcccg
     1681 cgcccgtttc gatcgacacc cgcaagccca aggtggccga cgccgcgctg cgcgcgggcg
     1741 ccaccatggt caacgacgtc actgccggcc gcgaccccgc catgatcgac gtgctgcgct
     1801 ggcgcgagga cgtgcccgtc gtgttgatgc acatgctggg tgagcccaag accatgcagg
     1861 ccgagccgcg ctacgacgac gtactgcgcg aggtcgggcg ctacttggac gagcgcgcgc
     1921 gcgccctgat cgaggccggt gtcgcgcgcg agcgcatcgt catcgacccc gggatcggct
     1981 tcggcaagcg cttcgaggac aatatcgcgc tgctcaaggg cctcgagacg ctgcgcaagc
     2041 tgggctaccc cgtgatggtg ggcgcttcgc gcaaggcatt catcgggaca ctgctggccg
     2101 gggacgccgg cgctcccgcg gcgggcgacc gcgtcgcggg cagcctggcc gtcgccgcgc
     2161 attgctacca cgccggagtg gagatgattc gcgtgcacga cgtgcgcgag accgttcagc
     2221 tgctgcgcgt cctcgacgcg gttcactccg actgacatgc cgcacattcc gatcgagctc
     2281 gtcgtcgacg tgatcgacat cgcgatcgtg tcgtttttgc tctaccacct cttcgccctc
     2341 atgcgcggca cccgcgccgt gcacatgttc ttcggcctca tcgttctgtt tctattgtcc
     2401 gtgatcgcac aatggatgaa cctgatcgcc gtcaattggc tcatctcgag cttgcgcacg
     2461 gcgtgggtga tcgcgttcgt gatcatcttc cagcccgagt tacggcgcgc tctctccatg
     2521 ctggggcaaa accgattgct ctcgcgcttc gtgaccatgc gccagagcca cgtcatcccc
     2581 gaggtggtca aggcggtgca ggagatggcc gagaagaaga cgggcgcatt gatcgtgctc
     2641 gaaaaagaca tgggcttgaa gaactacgtc gagaccggca ccaccgtcga cgcgcgcgtg
     2701 tcggccgagc tgatcgaatc gatcttcacc cgccccagcc ccttgcacga cggcgccatc
     2761 gtgatccaga acgaccgcgt ggtggcggcc gggtgcacgc tgccgctctc gcaggaccag
     2821 cgagccgcgc agtcgatggg catgcggcac cgcgcggcgc tggggctctc cgaagagacg
     2881 gacgccttgg tggtcgtgat atccgaagaa tcggggtcga tcgcctacgc ggaggacgga
     2941 aaactctacc gtaagatcga cctgaccacg ctcaagaacg agctgtttcg gagcttcggg
     3001 gtggccgggg aggagagagg ggccgcgggg taggctttcg ttcaactagt aacgtgcccc
     3061 ttcgggcaga atttgacgct acctcttcag taaagtagca gtatgattgg ggacattgcg
     3121 atgtcccttc gtccatgaca cgcgaggaac cgaaaccaac ccaaggctcc gtcagaggtc
     3181 tacgtgagta gaatacgctt gctgcccgca accgtcatcg tccttcttgc ttcctcaggt
     3241 gccaaagcgc aacttccgac ggttgtaacc gggggcgacg tatttgttta cgcaacgttc
     3301 gtctcgcctc caggaacgat ctcgtttgat gccttcgaca acatgttcgt cggcaatttc
     3361 aacaatcctg ggccgccgaa tgggccggcc cctgtttggg tcgtagatgc cgaagattcg
     3421 acagtcagca cctgtgcgac gatcgacgat ccagatgcgg tctatatcga tcgaaacggc
     3481 ctactttccc tttccgggac gcttctggtg ggaggattga tcagcccgac aacgaccggt
     3541 cagattagag gcgtaaccaa ctgcggcggg tcatctctgc tggcgtccgg cggatgcgtc
     3601 cacaatgtgc ggatgttcgc cgaagatgcg ctgggcaacc tatacgtcgc caatgcgacg
     3661 tcgagaacga tttgccgctg gaacggcgct acttgggcgc agtttctgcc cgcgtttcca
     3721 ctggccggtc cgatcgaacc tgcgatcgca atcgatggga ctgacgtcta cgtgtccgcg
     3781 gcgggagttg tgaaactcta tgacacatcg ggagcggtgg tcgaccctgc attcgctgtg
     3841 ggggaagtcg ttggcatagg gcccttcgcg acggctttcg agggcgtcat tgtctcacgg
     3901 gcggattcgc tcgaactggt ggccgtcgat ccggacacga aacgcgagag accgatcttg
     3961 actggcccgg gctcgccagg cgttgtcgca tttgattccg cgggcgacct ctacgtcgca
     4021 caggactacg cgaatcgcgt gctgcacgtg acacaaccca ttccgacgca tgtacccggc
     4081 gtaatctcga ccagaacatg cacgctctac caaaatgtcc cgaacccgtt caacccgacc
     4141 acgaccatac aattcgagat tccacgcagc gccagcacac agctggacgt ttttgatgtg
     4201 tcgggcaggc atgttcgttc gctcttgaga ggcatgatag gcaaggggcc acacgcagtt
     4261 acatgggatg ggacaaatca ccgtggcgag ctcgtgggat ccggcgtcta tttctacagg
     4321 ctagaagttg gtggcgaaag actgacgagg aagatggtgc tgctgaaata agcgcggggg
     4381 ccacgcgcca cgtggagtgg attctagtcg acgacccgcc gagaggcaaa cgggcaagtc
     4441 aagtcggagg taaacgtcta gacgctactt aaggacgacc agtttcacag tttccaaggc
     4501 tcccccccta cgaagcgcgc agtagtacac cccagacgtg acagcctttc catcgacgcg
     4561 acaccccctg ggcaagactg gaaccttcgc caaacaggat tcgcttgtct gtcgcgaacg
     4621 gaacccaaaa tatcaaaatc ggttacggac acatagctgc cgacttggac atcccgtcca
     4681 cactggtacg cctggtgacc ggaagtagat acccggcgtc gtttcatcgt gggaaggcgg
     4741 aggcggccgt atggcggggc cgtcgctttg actcccgttc gacttcgtgg tagcctgatt
     4801 gggctagagt tcactccacg accccgaacc atatgtctat cgacgatctc atccgggccc
     4861 acaacgccgg ggaggccgcg gctacgtcgc atctcctcga gctcctctat gcggaactcc
     4921 gccgtatcgc gtcgcgcttg atgtcgcgcg agaggcccgg ccacaccctg caaaccaccg
     4981 cgctcgtcag cgaggcctac ctgcgcctgg tcggcggtgg atcgaccgag atcagcgatc
     5041 gcaaacattt cctgtgcctg gccgcgacca ccatgcgcaa catcctcttc gatcacgcgc
     5101 gtgagcggga tgcgatcaag cgcggcggag gcgcggccca cgtgacgctc gatcactcgt
     5161 ggctcgcggc accggaggag agcatggata cccaagcact caaagaagcc atcgaagcac
     5221 tgtccaagga gtacccgcgc cacgtacgcg tcatcgatct gcgctacttc gtcggcttct
     5281 cggtcgaaga gaccgccgac ctactccaag tctgcgagaa gaccatcaag aacgataccc
     5341 aattcgcgaa ggcctggctg cgccgccggc tggtcgcgta gcgcgaaacc ctcatggacg
     5401 cggaacgaag cgctcgcgtc tccgatctct tcgatcacgc caaggaactt ccgcccgcgg
     5461 agcgcgacgc gttcctggcc cgcgagtgcg cgggcgacac cgagttgctg cacgaagtcc
     5521 gctcgctgct cgaccaccac gtcgacgaac cggagtcgcg cttcatcggt tggaacgcga
     5581 accacgcccc cgcggtcgac gacgagatca aaccgggcga cgtcgtccaa cgtttccgca
     5641 tcgcgcgaac catcggcgaa ggcggcatgg gcatcgtcta cctggccgag cagtcctcgc
     5701 cggtgcgccg caaggtggcc ctcaagatcg tccgcgtcgg catggacacc aaggaactcc
     5761 tggcccgctt cgaagcggaa cgccaggcgc tcgcgatgat ggaccacccc gccatcgcgc
     5821 gtatctacga cgcgggtgcc accgagcgcg ggcgcccgta cttcgccatg gagtacgtgg
     5881 acggcgtgcc gatcacggaa tactgcgacc agcgccggct cgatgtcaca tcgcgcctgc
     5941 gcctgttcgc cgacgtgtgc aacgcgatcc accacgcgca ccagcgcggc atcattcatc
     6001 gcgacatcaa gccgaccaac gtgctggtca ccgagtacga gggcaagccc gctcccaagg
     6061 tgatcgattt cggcattgcg cgcgcgaccg accagtcgct cgcggccgag acgatgcaca
     6121 cgcgaaggga cgtcatgctg ggaacgccgg cctacatgag tcccgagcaa gccgacccga
     6181 tggagccgcc cgacacgcgc accgacgtgt actcgctggg cgcgctcttg taccagctgc
     6241 tgacggggag accgccgctc gagttcggcg aacgccagac ggtcgagtcg attcgaagga
     6301 cgatcatcga taccgaccct ccgcgcccca gcgctcggct gacctcgctg ggcgagaagg
     6361 ccgccgcggt ggccgcggag cgccggaccg acgccaagca cctgctcagt ctcctgcgca
     6421 acgacgtcga ctggatcgtc atgcgcgcgc tcgagaagga ccgcgcgcgc cgctacggct
     6481 cggcctccga gctggccgcc gacatccaac gcttcctcga tcgtgaaccc gtggtggctc
     6541 gcccgcccac ggtgggctat cgcgtgcgca agtacgcgcg ccgacaccgc tacctcatgg
     6601 ccggcgcgac ggccgtgttc gtggtgctgg tggcgggatt gaccgcgacc atttggcaag
     6661 cgcgcgaggc gcggcagcga acgagagagt tggaagcgat cgcggcgcga ctcgtctccc
     6721 acttctacga gttgagcgac tacccgggag cgatggcggc gaagcaaacg ctcgcggaac
     6781 tcgccacgca aaccctcgac gatttgacga agcgaggagg cgacgatccg tcggtccggc
     6841 gccagctcgc gtccgcctac gaaggcctgg gtcagttact gggggacaac tactccgcga
     6901 gcctggggca atccgcggcg gccgtggtcg cgcgtaagaa agcgctcgat attcgcgagg
     6961 agctgcaccg cgcaaacccg gccgatttcg gtctcgccaa cgaatacttg aagagctacg
     7021 cggcctgggg aatgctggct ctcttcggat acctggacga ggtcggtatc cccggggtcg
     7081 catggcaccc cgaagtcatt cccaccgcgc agcagcggcg cgaccgcaag atgatcctat
     7141 ccctctccga cgacgtcgcc tccgagcgcg cgtggttcct cggtcgcacc gactcgatgg
     7201 tgttctacat cgaacgcgcg cgcgcgcgcc gcgaggcgct gctcgcggaa gagcccggca
     7261 acctcgagta ccagcggctc ctggccgaca cctacgatcg cctcgcgtac ctcacgcgcg
     7321 acgtcgacga agccgagcgc ctcgcgcgac gcgccgtcga gttctgcgag aactactacc
     7381 gcgcgcaccc gttcagctgg aaggccagca tggatgtcgc ctacacgcgc cgcaacctcg
     7441 gctatgcgcg gtggcgaaaa ggcgacctcg cgggcggtct cgaagagcag cgcctgggcc
     7501 tcgaagcgcg tcgccgcctg gccagcgcgg acagcatgaa cgtgcgcggc acgtttctct
     7561 tgagcgaagg ctactcgctg gtcggccagg cgttcttgcg gatgggccgg cccgattcgt
     7621 cgattcacta ttcgcgccaa gccctgggac tactgtacga gctggccaag tcacccaacc
     7681 cgcccgactg gacggggaac gagctggcct tcgtgcacac ctacctcgcg aacgcgcacc
     7741 aagaccggaa cgacgccgaa tc
//
LOCUS       DASPGK010000180         6714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1333405, whole genome shotgun sequence.
ACCESSION   DASPGK010000180 DASPGK010000000
VERSION     DASPGK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333405"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VEC56_05160"
     CDS             <1..692
                     /locus_tag="VEC56_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXV13575.1"
                     /translation="NIGERYVLYKKFMAFAWGNGGDVFDETGTVVLNSPAVLEALEFY
                     LTLAPFSLQEKQEVLDQYFKTGRLGMQVSGAWNLKNYKLEAPDLDYSVALVPKPAADH
                     GSHASFAGAEMLVVFKASPRKDAAVALARFLQAYPQAKTLSLAAGSVFPAARAAMNDP
                     TFTSDPRVRVFVEQSLTSRTTPAHPGWIEMEEAIDRAVEESLYGRRAPRWCLDDAAKE
                     VQQIAARFPKK"
     gene            699..1592
                     /locus_tag="VEC56_05165"
     CDS             699..1592
                     /locus_tag="VEC56_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXV13576.1"
                     /translation="MATRRFVLPPVAWFLLPWAVVYAAFFVFPFVFSFALAFLDYNPL
                     NVAGTRFVGTENFARLFGDAAFHGALRNTVLFVVGTVPLTTAAALALAGLMRGSIPLK
                     GAFKAALFLPSILSVVVISLLFKLFYAPGGALNEALARFGLAGHGWLTDPSTALPAIM
                     VMDVWAAVGYYAIVLDAARTSIPDALYEAAELEGAGAWATFRHVTFPLLKPAIAFVLV
                     INTIRSFQVFVEVLVMTQGGPLGSTNTLVLYLYDTAFRRLDYGYASVVAYAVFLLSGL
                     FSIVAMRRFRSETAVEGARPL"
     gene            1589..1801
                     /locus_tag="VEC56_05170"
     CDS             1589..1801
                     /locus_tag="VEC56_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13577.1"
                     /translation="MMRVMRMVLLVALVAVMAYPLAWMIAISLKTADGPSLRYYGEVW
                     QAGPFDRYFINSFVVAAIVLVGNIVC"
     gene            1805..2362
                     /locus_tag="VEC56_05175"
     CDS             1805..2362
                     /locus_tag="VEC56_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXV13578.1"
                     /translation="MAGYAFARFRIRGGKFLFLLVLSTIMLPKQVILVPLYILMQKMG
                     LIDTYWALTLPFLVDPFNIFLIRQYVSTLPADCEEAARIDGAGEFTILFRVVFPMLKP
                     VLAVVAIHTCLTNWNSFLFPFILTHSSNMRTLPVGLAMLSQGAHSIDWGHLMAGAVIS
                     ALPVVAAFLVFQRRIIGGLTAGISK"
     gene            2368..3561
                     /locus_tag="VEC56_05180"
     CDS             2368..3561
                     /locus_tag="VEC56_05180"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HXV13579.1"
                     /translation="MSNHVWPSLVGKDHFTCVGLMSGTSMDGVDAALVAMSASPEHPR
                     VELLEFVSLPYPGSLRESLADLAGGAAVTAEEVALLSTGVAVAFAGGFFDVCRRAGKE
                     PRVIDFVGSHGQTVAHVPPGSGSPVAGTLQIGSPAMIAALTGVTTVGDFRSGDVAIGG
                     QGAPLAPYCDYMLRRSSTASRVILNIGGISNLTYLRKGGSRESVIAFDAGPGNMVADT
                     LFRALFPGHGAYDEDGVRAGAGTVSRDVVERLMRHPYFGASPPKSAGHREFGPPFAWA
                     LKTAADAENASPEDVLATAVALTVESIVDAMRRFLPEGGVDEVFVTGGGARNRAMLAS
                     LEVALAPVAVRPVDELGIPAEAKEAVDFAFLAREALLGRRNVLRAVTGASRELVLGTI
                     ARGSQ"
     gene            3558..4808
                     /locus_tag="VEC56_05185"
     CDS             3558..4808
                     /locus_tag="VEC56_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXV13580.1"
                     /translation="MTLMKMASVALATAGLWVPLGGPASAAQTRVEPTIRVLVFSTSG
                     SATVATTAGFRVLAGGALKFESDRGGTVSVYRDGRTLTVRHDATSQSASNETEVTIEP
                     VEKPLSVGGVWYPGSIRALPREDGGVDLVSLVPLETYLEGVVPHEIGEPGPDAYAAVQ
                     AQAIAARTYAMSRMKLNRDKPYDVEAGVMDQVYRGNEKRGRMTSSAVYDTRGLVLMHD
                     GDLCDAYYSATCGGHTSDIRKIWPDRPSAPYLHGRRDRGEEGGESFCAAARNFRWRYS
                     FSGRALGDTVRRTLPMVAGVDESEVGELVDVRVRRRSASGRVQELEIVTSTRSFVVEN
                     DKIRRVVMLDVGKGRILPSTLFDVETTMNGARVGIVSFAGGGNGHGVGMCQNGALGMA
                     RKGYSYSMILQHYYPGAELRPGYK"
     gene            4805..6604
                     /locus_tag="VEC56_05190"
     CDS             4805..6604
                     /locus_tag="VEC56_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXV13581.1"
                     /translation="MNKEHDVLTPEVRFHLIRKMERHKVIALTGPRLLPHERKYLEFH
                     SVSGVILFGRNIESLSQVGDLIGGVCEELAREGLTPLVMADHEGDFVAELRQIIGVPP
                     APLAIAATGDLDLAAEVAYETGKAMRKLGANVVLAPVADCYLESPSAITGLRTFGADP
                     GRVAAFVEQTIAGFREAGVLSCAKHFPGHGSTAEDSHETLPEVRKSAEDLAAADLIPF
                     RRSIGVGVDLMMMSHVAYPMGREELIPASFDARIIRGMLRTELNYDGVVITDALEMAG
                     ARWYASERSGMVTGGNERSLLAGSDLLLHTKPIPEQVQVERGSEPVMSLNVMETIVRT
                     LEKIVDRGRVDEKLAEAAAESEPLRNVLSILDASSARVTKLRERAADPRASARPAGGK
                     VIELNAYPSVPTVYRTVAERSITAGSDWVRFQALAPDSTCVVLPVEWSPSETLKKQDM
                     AGFLDVVCRHFPRWHRMPLVTEFETDAEGSLRPVFQRERATVVDAARFAGGTGAAREL
                     ANEEVVMVFSSRGAPSEEFALGLTALADEYRPSVVVLTGWIVDDWVPEGTPIVISLGA
                     STQVASAVAQVLAGALVPEGSLAGLLPAAYPRP"
BASE COUNT         1028 a         2184 c         2286 g         1216 t
ORIGIN      
        1 agaacatcgg cgagcggtac gtcctctaca agaagttcat ggccttcgcg tggggcaacg
       61 gcggcgacgt cttcgacgaa accggcaccg tggttctcaa cagccccgcg gtcctggagg
      121 cgctcgagtt ctatctcacg cttgcgccgt tctccttgca ggagaagcag gaagtcctcg
      181 accagtattt caagaccggg cgactgggaa tgcaggtctc gggcgcttgg aatctcaaga
      241 actacaagct cgaggccccc gacctcgact attcggtggc gctggtaccc aagcccgccg
      301 ccgatcacgg ctcgcacgcg agcttcgcgg gcgcggaaat gctggtggtg ttcaaggctt
      361 cgccgcgcaa ggacgcggcc gtggcgctcg cgcgcttctt gcaggcttat ccgcaggcga
      421 agacgctctc gctggccgcg ggcagcgtgt ttcccgccgc gcgcgccgcc atgaacgatc
      481 ccacgttcac cagcgacccg cgcgtgcgcg tgttcgtcga gcagtcgctc accagccgta
      541 ccacgcccgc ccacccgggg tggatcgaga tggaagaagc catcgatcgc gcggtcgaag
      601 aatcgttgta cggccgccgt gccccgcgct ggtgcctcga cgacgccgcc aaagaagtgc
      661 agcagatcgc cgcgcgcttc ccgaagaagt aggtgggtgt ggcgacgcgt cgattcgtcc
      721 tcccgcccgt cgcgtggttc ctcctgccgt gggcggtcgt gtacgccgcc ttcttcgtgt
      781 tcccgttcgt gttctcgttc gcgctcgcgt ttctcgacta caacccgctc aacgtggcgg
      841 gcacgcgctt cgtggggacg gagaacttcg cacggctctt cggcgacgcg gcctttcacg
      901 gcgcgctgcg caacacggtg ctgttcgtgg tggggacggt acccttgacg accgcggccg
      961 cgctggcgct cgcgggactc atgcgcggat cgattccact gaagggcgca ttcaaggccg
     1021 cgctgttcct gccttcgatt ctctcggtgg tggtgatctc gctcctgttc aagttgttct
     1081 acgcgccggg cggggccttg aacgaagcgc tcgcgcgctt cggcctcgcg gggcacgggt
     1141 ggctcaccga cccgagtacc gcgctcccgg ccatcatggt catggatgtg tgggcggccg
     1201 tgggctatta cgccatcgtg ctggacgccg cgcgcacgtc gattccagat gcgctctacg
     1261 aggcggccga actggagggc gcgggtgcgt gggcgacgtt ccgccacgtg acctttccgc
     1321 tcttgaagcc ggccatcgcc ttcgtgctgg tgatcaacac cattcgctcg ttccaggtat
     1381 tcgtcgaagt gctcgtgatg acgcagggcg gcccgcttgg atccaccaac acgctggtgt
     1441 tgtatctcta cgacaccgcc tttcggcgcc tcgactacgg ctacgcgagt gtggtggcgt
     1501 acgcggtgtt cctgctctcg ggcctcttct cgatcgtggc catgcggcgc tttcgatccg
     1561 agaccgcggt ggaaggagcg cgcccgctgt gatgcgagtc atgcgcatgg tgctcttggt
     1621 cgccctggtg gcggtgatgg cgtatccgct ggcgtggatg attgccatct ctctcaagac
     1681 cgcggacggc ccgagccttc gatactatgg agaagtgtgg caggcgggac ccttcgaccg
     1741 ctacttcatc aatagcttcg tggtcgcggc catcgtgctg gtcggcaaca tcgtgtgttg
     1801 aacgatggcg ggctacgcct tcgcgcgctt ccgcatccgg ggcggcaagt ttctgttcct
     1861 gctcgtgctc tcgaccatca tgctgcccaa gcaggtgatc ttggtgccgc tctacatatt
     1921 gatgcagaag atggggctca tcgacaccta ctgggcgctg acgctgccgt tcctggtcga
     1981 cccgttcaac attttcctca ttcgccagta cgtctcgacg ctgccggccg actgcgaaga
     2041 agccgcgcgc atcgacgggg ccggcgagtt cacgatcctg tttcgcgtcg tgtttccgat
     2101 gctcaagccg gtcttggcgg tggtggcgat tcatacctgc ctcaccaact ggaactcgtt
     2161 cttgtttccg tttatactga cgcacagttc caacatgcgc acgctcccag tcggtctcgc
     2221 gatgttgtcg cagggcgcgc acagcatcga ttggggtcac ttgatggcgg gggccgtcat
     2281 ctcggcgttg ccggtggtgg cggcgtttct cgtgttccag cgccgcatca tcggcggcct
     2341 caccgcgggc atctcgaaat agaccaaatg tcgaatcacg tctggccatc gctcgtcgga
     2401 aaggatcact tcacgtgcgt ggggctcatg tcgggcacgt cgatggacgg cgtcgacgcg
     2461 gccctggtcg caatgtcggc gagtcccgag cacccgcgcg tcgagctgct cgagttcgtc
     2521 tcgctgccct acccggggtc gctgcgcgaa tcgctcgccg acctggcggg gggcgcggcc
     2581 gtgacggccg aagaggttgc gctgttgtcg accggcgtcg cggtggcctt cgccggcggc
     2641 ttcttcgacg tgtgccggcg cgcgggcaag gagccgcgcg tgatcgactt cgtgggctcg
     2701 cacggacaaa ccgtcgcgca cgtacctccg gggagcggct cgccggtggc gggaacgctc
     2761 cagatcggtt cgccggccat gatcgccgcg ctcaccggtg tcaccaccgt cggtgacttt
     2821 cgcagcggcg acgtcgccat cggagggcag ggcgcgccgc tcgctccgta ttgcgactac
     2881 atgctgcgaa ggagcagtac ggcgagccgc gtgatcctga acatcggcgg catctcgaac
     2941 ttgacgtact tgcgcaaggg cggttcgcgc gaaagcgtga tcgccttcga cgcggggccg
     3001 gggaatatgg tggcggacac gctgtttcga gcgctgtttc cgggccacgg cgcgtacgac
     3061 gaagacggtg tgcgagccgg cgcggggacc gtttcgcgcg acgtggtcga gcggttgatg
     3121 cggcacccgt acttcggcgc ctcgccgccc aagtcggcgg gccatcgcga attcggaccg
     3181 cccttcgcgt gggcgctaaa gaccgcggcg gacgcggaga acgcctcacc cgaggacgtg
     3241 ctcgccaccg cggtcgcgct caccgtcgag tcgatcgtcg acgccatgcg ccggtttctg
     3301 cccgagggag gtgtggacga ggtattcgtc accggtgggg gtgcccgcaa ccgcgccatg
     3361 ctcgcgagcc tcgaagtggc cctggcgccg gttgcggtgc ggcccgtcga cgagctgggc
     3421 atccccgccg aagccaagga ggcggtcgac ttcgcgttcc tcgcgcgcga ggcattgctc
     3481 gggcgacgca acgtgctgcg cgcggtaacc ggcgcttcgc gcgagttggt tctggggacg
     3541 attgcaaggg gttcgcaatg acgctcatga agatggcatc ggtcgcgctc gcgaccgcgg
     3601 ggttgtgggt cccgctgggc ggtcccgcca gcgccgcgca aacgcgcgtc gaacccacca
     3661 ttcgcgtgct cgtgttctcg accagcggtt cggccaccgt cgccaccacc gcgggcttcc
     3721 gcgtcctcgc cggcggcgcg ctcaagttcg agagcgaccg cggcggcacc gtctcggtgt
     3781 accgcgacgg ccgcaccttg accgttcgcc acgacgccac cagtcagagc gcgtccaacg
     3841 aaaccgaggt caccatcgag ccggtcgaga agccgttgtc ggtgggtggg gtatggtatc
     3901 ctggcagcat tcgcgcactg ccgcgcgagg acgggggagt ggacctcgtg agcctggttc
     3961 cgctggagac ctatctcgag ggggtcgtcc cacacgagat tggcgagccc gggccggatg
     4021 catacgccgc ggtccaggcc caggccatcg ccgcgcgcac ctacgccatg tcacgcatga
     4081 agctcaatcg ggacaaaccg tacgacgtcg aggcgggagt gatggaccag gtctatcgcg
     4141 gcaacgagaa gcgcggccgt atgaccagct ccgcggtcta cgacacgcgc ggactcgtgc
     4201 tgatgcacga cggcgacttg tgcgacgcgt attacagcgc cacctgcggt ggtcacacct
     4261 ccgacattcg gaagatctgg cccgatcgtc cgtcggcgcc ctacttgcac ggacgccgcg
     4321 accgcggcga agagggcggc gagagcttct gcgccgcggc ccgcaacttc cgctggcgct
     4381 actcgttctc ggggcgcgcg ctgggagaca cggtgcggcg aacgctcccc atggtcgcgg
     4441 gtgtggacga gtcggaagtg ggcgagctgg tcgacgtgcg cgtgcgccgg cggagcgcct
     4501 cgggacgcgt gcaagagttg gagatcgtga cttcgacgcg gtcgttcgtg gtggaaaacg
     4561 acaagattcg ccgcgtggtc atgctggacg tggggaaagg gcgcattctc cccagcacgc
     4621 tcttcgacgt cgagacaacc atgaacggcg cgcgcgtggg catcgtgagc ttcgcgggcg
     4681 gcgggaacgg ccacggtgtc ggcatgtgcc agaacggcgc cctcggcatg gcgcgcaagg
     4741 gctactcgta ttcgatgatc ctacagcact actatcccgg cgcggagttg cggcccggct
     4801 acaaatgaac aaagagcacg acgttctcac tccggaagtt cgcttccatc tgattcgaaa
     4861 gatggagcgg cacaaggtca tcgcgctgac cggaccgcgt ctgttgccgc acgagcgcaa
     4921 gtacctcgag ttccattccg tttcgggtgt gattctgttc gggcgcaaca tcgagagcct
     4981 ctcgcaagtg ggggatctca tcggcggtgt gtgcgaagag ctcgcgcgcg aagggctgac
     5041 cccgctcgtc atggccgacc acgagggcga cttcgtggcc gagttacggc agatcatcgg
     5101 ggtgcccccg gcgccgctcg cgatcgcggc caccggggac ctggacctcg ccgctgaggt
     5161 cgcgtacgaa accggcaagg cgatgcgcaa gctgggcgcc aacgtggtgc tggcgccggt
     5221 cgccgactgc tatttggaat cacccagtgc tattaccggg cttcgcacct tcggcgccga
     5281 ccccgggcgc gtggccgctt tcgtcgagca gaccatcgcg ggctttcgcg aggccggtgt
     5341 cctctcgtgc gcgaagcact tcccgggcca cggttccacc gccgaggact cgcacgaaac
     5401 gctccccgag gtgcgcaagt ccgccgaaga cctcgcggcc gcggatctga ttccgttccg
     5461 gcgctccatc ggggttggcg tcgacctcat gatgatgtcg cacgtcgcct accccatggg
     5521 ccgcgaagaa ttgataccgg cgagcttcga cgctcggatt atccgtggca tgctgagaac
     5581 cgaattgaac tacgacggcg tcgtcatcac cgacgccctc gagatggcgg gcgcgcgctg
     5641 gtacgcctcc gagcgctccg gcatggtgac gggcggcaac gaacgctcgc tgctggccgg
     5701 ctcggacttg ctgctgcaca ccaaacccat ccccgagcag gtgcaagtcg agcgcggtag
     5761 cgagccggtg atgtcgctga acgtcatgga gaccattgtt cgtacgctgg agaagatcgt
     5821 cgaccgcgga cgggtggacg agaagctggc cgaggcggcc gccgagagcg agcccttgcg
     5881 caacgtgctc tcgatcctgg atgcatccag cgcgcgcgtg accaagctgc gcgagcgggc
     5941 cgccgacccc cgcgcctcgg cgcgacccgc gggtggaaag gtgatcgagc tcaacgcgta
     6001 cccgtcggtt ccgaccgtgt atcgcacggt cgcggagcgc agcatcacgg ccgggagcga
     6061 ttgggtgcgc ttccaggcac tcgcaccgga ctcgacctgc gtcgtgctgc cggtcgaatg
     6121 gtcgccgagc gagaccctca agaagcagga catggccggt ttcctcgatg tggtgtgccg
     6181 gcacttcccg cgctggcacc gcatgccgct cgtcaccgag ttcgagaccg acgccgaagg
     6241 cagtctgcgc cccgtattcc agcgcgagcg cgcgacggtg gtggacgcgg cgcgcttcgc
     6301 gggcggtacc ggcgccgcgc gcgaactggc gaacgaagag gtcgtgatgg tgttctcgtc
     6361 gcggggagcg ccctcggaag agttcgcgct gggactgacc gcgctcgccg acgaataccg
     6421 cccgagcgtg gtcgtgctga cgggctggat cgtcgacgac tgggtgccgg aaggaacgcc
     6481 gatcgtgatc tcgctcggcg catcgacgca ggttgcatcc gcggtcgcgc aagtgctcgc
     6541 gggtgcactg gtccccgaag gatcgctcgc cggcctcttg cccgccgcct atccgcgtcc
     6601 ctgacaccgg cttaaagtat tgaaaagacg tagaataggg gattgacacg gcgccgtgtc
     6661 ggtgctagct tggagtccgc gcgggaagct ccttccactc cgcgcgcttg cttt
//
LOCUS       DASPGK010000181         4453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_982440, whole genome shotgun sequence.
ACCESSION   DASPGK010000181 DASPGK010000000
VERSION     DASPGK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982440"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VEC56_05195"
     CDS             <1..993
                     /locus_tag="VEC56_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13582.1"
                     /translation="FFLRQRSPHRIQRFTCLGCHRHFSTQTFSTSYWQKRPELMGRIV
                     MMVVGCMGNRQMARALLCSPATVAHQVARLGRHCLLVQANELGRIDSLREIAIDGFES
                     FEWSQYFPFHHNVAVDVESGYFLYHTDSPLRRKGRMTAHQKARRAYLETSLGRPDPRA
                     VEAGVRDLLAPLVSRRVASIRSDDHRAYPRAIEGLGGSIAHRITSSKERRDQRNPLWE
                     VNLLDLMIRHSTAAHKRETIAWAKRRQASIEKLTIFQVWRNYIKRRHEKGARVTPAMR
                     LGLTTRPWRIRDLLARRLYVERTGLTNRWEEYYRRRVVTRALAVNRTHELRYAY"
     gene            complement(1070..3796)
                     /locus_tag="VEC56_05200"
     CDS             complement(1070..3796)
                     /locus_tag="VEC56_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HXV13583.1"
                     /translation="MDSERFRRVQEVFHAALDAPARDRERFVFEACREDWSLYREVLS
                     LLDHHATDDWTPGDDPATAGRPSQRAREPDSARALTNGNERYELVDILGEGGMGIVYL
                     AEQTHPIRRRVALKVIKLGVDSRRATARFESERQALALMDHPNIARVFEAGATEDGRL
                     YFAMEYVPGRPISEFCDQAKLSVDERVRLFTTVCDAVQHAHQKGIIHRDLKPSNILVA
                     TQGASPEPKIIDFGVARAMNQRLTEHTMFTQLGVVVGSFRYMSPEQADCRLLEIDTRT
                     DIYALGAVLYELLTGDTPIDAPTIEQTGYAEVQRRIREEEPAKMSERAQRLAGEDAAT
                     VATQRGTDLASLSRRLKGDLDWIVLKALDKERTRRYATAAEFSDDLRRHLANEPVLAG
                     PPTRAYRLRKFVRRNRVPVVAGTAVALAVVLGLVASTTLFLENRASRRVAEAQRDQIL
                     RLSDANVLAQLESTVDDLRPPHPERVPAMEEWLADADVLASRLPAHRATLAALRAHAL
                     HADAPAPPSFASQEDQFHFDHLSTLVADLERFVDPDPHVGSIARVRERHAFATSVMEE
                     SIHSKRREWNDAIRSIASIDESPRYGGLVIAPQVGLVPLGRDRASGLWEFVHIRTGKA
                     PVRGRDGRWVMAEETGIVLVLLPGACYWMGMSKERLPDPAMPSGNPLARFDEVPVHRV
                     CLDPFFISKYEVTQAQYQRMTGANPSRVRPGIDYGMYVTSLRHPVENLDWAMADAFSR
                     DVGLELPTEAQWEYAGRAGSTARFPCGWDPACLARTENILDASARVPARPGGWSYASW
                     HDGFVDSSPVGFFAPNAFGLHDVMGNVTEWTRDWYGSYQLPVAPGDGERLGGAQAERV
                     VRGGGFYFWPDRVNLALRLNLQPHINDRIGVRPARAVDPADRVAGASARPSR"
     gene            complement(3789..4352)
                     /locus_tag="VEC56_05205"
     CDS             complement(3789..4352)
                     /locus_tag="VEC56_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXV13584.1"
                     /translation="MTNESDHNHADASAIERILPTVYEALRELAHRRLLSERKGHTLQ
                     TTELVHEAYLRLQSGGNAPWNSDAHFYCAAAEAMRRILIERARRRKRLRHGGGLVRLN
                     IEDQDVAADVAGEDIIAISDALDELEKHDRRVADVVRLRFFVGFTVEETAEKLGVSPA
                     TVKLDWSFARAWLQRAIAEGRRGDERG"
BASE COUNT          693 a         1477 c         1549 g          734 t
ORIGIN      
        1 ttcttcctgc gtcaacgctc cccgcatcgt atccagcggt ttacatgctt ggggtgtcat
       61 cgtcacttta gtacgcagac cttctcgacc tcctactggc aaaagcgccc cgagctgatg
      121 ggtcgcatcg tcatgatggt cgtcgggtgc atgggcaacc gacagatggc ccgagccctc
      181 ctgtgcagtc cagccaccgt ggctcaccaa gtcgcgcgtc tcgggcggca ctgtctgctc
      241 gtccaggcga acgaactggg tcgcatcgat tcgttgcgtg agatcgcgat cgacggtttc
      301 gagagcttcg agtggagcca gtactttccg ttccaccaca acgtggcggt ggacgtggag
      361 tcgggatact tcctctatca caccgacagc ccgcttcgcc gcaaggggcg catgaccgcg
      421 caccagaagg ctcgtcgcgc ttatctcgag acctccctgg ggcgcccaga tccgcgggcg
      481 gtggaggccg gggtgcgcga cctgctggcg cctctcgtct cgcgccgtgt cgcatcaatc
      541 cgaagcgacg accatcgcgc ttatccccgg gcgatcgaag gccttggtgg cagcatcgcc
      601 catcggatca cgtcctcgaa ggagcggcgg gatcagcgca atccgctctg ggaggtcaat
      661 ctgctcgacc tcatgatccg ccacagcaca gccgcccaca agcgggagac gattgcgtgg
      721 gcgaagcggc gtcaggcctc gatcgagaag ctcacgatct tccaagtgtg gcgaaactat
      781 atcaagcgac ggcacgagaa gggagcgagg gtgaccccgg ctatgcggtt gggactcacg
      841 actcgaccat ggaggatccg ggatctactc gcgagaaggt tgtacgtcga gagaacgggg
      901 ctaacgaatc gttgggagga gtactatcgc cggcgggtgg tcacgcgcgc tctggcggtc
      961 aaccggaccc acgagctgcg gtacgcgtac taaccgacgt gattacttca ctcgagagcc
     1021 atcaacgtga tcgcgaggga caatctcgcg gccacaaccg atttcttggc tagcgggaag
     1081 gacgcgccga ggcgcctgca acccgatccg cggggtcgac cgcgcgcgcc gggcgcaccc
     1141 cgatgcggtc gttgatgtgg ggctgtaagt ttaaccgtag tgctaggttg acccggtccg
     1201 gccagaagta gaacccgccc ccgcgaacca cgcgctcagc ctgggcaccc cccaaccgct
     1261 ccccgtcgcc cggtgccacc ggcagttgat acgagccgta ccagtcccgc gtccactcgg
     1321 tgacgtttcc catcacgtcg tggagaccga aggcgttggg cgcgaagaac cccaccggcg
     1381 acgaatccac gaatccgtcg tgccaggagg cgtagctcca gccgcccgga cgcgccggaa
     1441 cgcgcgcgga cgcgtcgagg atgttctcgg tgcgcgccag gcacgcgggg tcccagccgc
     1501 acgggaagcg cgcggtcgat cctgcgcgcc ccgcgtactc ccactgcgcc tcggtgggaa
     1561 gttcgaggcc gacgtcgcgc gaaaacgcgt cggccatcgc ccagtccagg ttctcgaccg
     1621 ggtggcgcag cgacgtgacg tacatcccgt agtcgatccc cggtcgaacc cggctcgggt
     1681 tcgcgcccgt catgcgctgg tactgggcct gcgtgacttc gtacttcgag atgaagaacg
     1741 ggtcgaggca cacgcggtgc accgggacct cgtcgaagcg cgcgagcgga ttgccgctcg
     1801 gcatggcggg gtcgggcaga cgctccttcg acattcccat ccagtagcac gcgccgggca
     1861 gcagcacgag cacgatgccg gtttcttccg ccatgaccca gcgcccgtcg cggccgcgca
     1921 ccggcgcttt cccggtgcga atgtggacga actcccatag gcccgacgcg cggtcgcggc
     1981 ccagcggtac cagccccacc tggggcgcga tgacgagtcc gccgtagcgc gggctctcgt
     2041 cgatgctcgc gatcgaccgg atcgcgtcgt tccattcccg gcgtttcgag tgaatgctct
     2101 cctccatcac cgaggtggcg aacgcgtggc gctcgcgcac gcgcgcgatc gagcccacgt
     2161 gcggatccgg atcgacgaag cgttcgaggt cggcgaccag cgtggaaaga tgatcgaagt
     2221 ggaactggtc ttcctggctg gcgaacgacg gcggcgcggg cgcgtcggcg tgcagcgcgt
     2281 gcgcccgcaa tgccgcgagg gtggcgcggt gcgcgggcag gcgcgacgcg agcacgtccg
     2341 catcggcgag ccactcctcc atggccggga cgcgctccgg gtgcggcggc cgcaagtcgt
     2401 ctaccgtgga ctcgagctgg gccagcacgt tggcgtcgga caggcgcagg atctggtcgc
     2461 gctgcgcttc cgcgacccgc cgcgaggcgc gattctcgag gaacaacgtg gtgctagcga
     2521 ccaaccccaa gaccacggcg agcgccaccg cggtcccggc caccaccgga acgcgattgc
     2581 gtcgcacgaa cttgcgcagt cggtacgcgc gcgtgggcgg acccgccagc acgggctcgt
     2641 tggccaggtg gcggcgcagg tcgtcggaga actcggccgc ggtcgcgtag cgccgcgtgc
     2701 gctccttgtc gagcgccttg agcacgatcc agtccaggtc gcccttgaga cgcctcgaca
     2761 gcgaggcgag gtcggtgccg cgttgcgtgg cgaccgtcgc cgcgtcctcg cccgccagcc
     2821 gttgcgcacg ctcgctcatc ttcgcgggct cttcttcgcg gatgcggcgc tgcacctccg
     2881 cgtagccggt ctgctcgatg gtgggcgcgt cgatgggcgt gtcgccggtc aacagctcgt
     2941 agaggaccgc gcccagcgcg tagatgtcgg tgcgcgtgtc gatctcgagc agccggcagt
     3001 cggcctgctc cgggctcatg taccggaagc tccccaccac gacgccgagt tgcgtgaaca
     3061 tggtgtgctc ggtcaggcgt tggttcatcg cgcgcgcgac accgaagtcg atgatcttgg
     3121 gctcggggga agcgccttgg gtggcaacca ggatgttcga gggcttgagg tcgcggtgaa
     3181 tgatgccctt ctggtgcgcg tgctggaccg cgtcgcacac cgtcgtgaac aagcgcacgc
     3241 gctcgtcgac cgacagcttg gcctggtcgc agaattcgct gatgggacgg cccggcacgt
     3301 actccattgc gaagtagagg cgtccgtcct cggtggcacc ggcttcgaag acgcgcgcga
     3361 tgttggggtg gtccatcagg gccaaggcct ggcgctccga ctcgaagcgc gcggtcgcgc
     3421 ggcgcgagtc gacccctaac ttgatgacct tgagggcgac gcgccggcga atggggtgcg
     3481 tctgctcggc gaggtagacg atgcccatcc cgccctcgcc gagaatatcg acgagctcgt
     3541 agcgctcgtt gccgttggtc agcgcgcgcg cactatcggg ttcgcgcgcc cgctgcgacg
     3601 ggcgcccggc ggtggccggg tcgtcgcccg gggtccagtc gtcggtggcg tggtggtcga
     3661 gcagcgagag cacttctcga tagagcgacc agtcttcccg gcaggcttcg aacacgaagc
     3721 gctcgcggtc gcgcgcgggg gcgtcgagcg cggcgtggaa gacttcctgg actctgcgga
     3781 agcgctcgct atccacgttc gtctccgcgg cgcccctcgg cgatcgcgcg ttgcagccac
     3841 gcgcgcgcga aggaccagtc gagtttgacg gtcgcgggcg aaacacccag cttctcggcc
     3901 gtttcttcga ccgtgaatcc gacgaagaag cgcaggcgca cgacgtcggc cacgcggcga
     3961 tcgtgcttct ccagttcgtc cagcgcgtcg ctgatggcga tgatgtcctc gccggccacg
     4021 tcggcggcga cgtcctggtc ctcgatgttg aggcgcacca gcccgccccc gtggcgcagc
     4081 cgcttgcggc gtcgcgcgcg ctcgatgagg atgcgccgca tggcttcggc ggccgcgcag
     4141 tagaagtggg cgtcgctgtt ccagggggcg tttccgcccg actgcaagcg caggtacgcc
     4201 tcgtggacca gctcggtggt ttgcagggtg tggcccttgc gctcggacag gaggcggcga
     4261 tgggcgagct cgcgcagcgc ctcgtaaacc gtgggaagaa tgcgctcgat ggccgacgcg
     4321 tcggcgtggt tgtggtcgga ttcgtttgtc atcgcatggg gccggcgacg tcgagtatgg
     4381 catcgacgcg caggcggtcg caaggggact gggtcgtcac cgcacgggcc gcaagcgcgc
     4441 gccggcccgc aca
//
LOCUS       DASPGK010000182         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_982475, whole genome shotgun sequence.
ACCESSION   DASPGK010000182 DASPGK010000000
VERSION     DASPGK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982475"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VEC56_05210"
     CDS             complement(<1..748)
                     /locus_tag="VEC56_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXV13585.1"
                     /translation="METRTVTLGALVDAAGPLALAPLDAPARALAVTGIAIDSRALRP
                     GNVFVAVPGTASDGHDFIDDAVARGASAVVVEHACPSARVPAVVVANSAQALAVMASR
                     FYGDPARDLFLCGVTGTNGKTSTTYLLRSIFQRAGRRMGIIGTLGHGVEQLVRDPHTT
                     PDAVTLHSWFRAMRDQNCFGVVMEVSSHAVRQHRVWGLDFSVGIITNVTHDHLDFHKT
                     MDDYKAAKAEFCHSLVAAGRHKPNGILVYWA"
     gene            complement(766..>2242)
                     /locus_tag="VEC56_05215"
     CDS             complement(766..>2242)
                     /locus_tag="VEC56_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXV13586.1"
                     /translation="PVDGKHVYLTIDATVQDVAETELRRAVEEFDARGGAVIVMEIAT
                     GDVLALAELPSIADRERASRADSLWTLRSVSHVYEPGSTFKLVTMAALLDKTRVTPAD
                     SFDAEDGKADIGVATIRDPHPHRWLTLDEAFMYSSNIVMAKASSYLQPGDLYGYARLF
                     GFGTKTGIGLPGESAGLVPPIERWSARTKATMAFGQEVAVTPIQILNAFAAIANDGVM
                     MMPRLVRGVADPETGDTSRSEPVVVRRVVSEETAATLRAMCLRVVEDGTAQAARLEFM
                     RVAGKTGTAQKAGSRGYLLHRYVSSFVGFAPYEKPKIAAIVMLDEPKWASRFGGDSAA
                     PVFARICRALATSTAWFDDVLSLETMRVASLDGRGATAPNFLRMDREAALSAARRLGA
                     NVLASGERGRVVAQEPAPGSPMDRDGVVRLFVMDASGVEKTGVDSNRRLRASYRDAVR
                     AGDDGDEQAPRARDARASNQRNAAGVVAAHNATARGSGQRR"
BASE COUNT          350 a          810 c          748 g          334 t
ORIGIN      
        1 cggcccagta caccaggatg ccgttcggct tgtgacggcc cgccgcgacg agcgaatgac
       61 agaactccgc cttcgccgcc ttgtagtcgt ccatggtctt atgaaaatcg aggtggtcat
      121 gcgttacgtt cgtgattatc ccgacgctaa aatctagccc ccatactcga tgttgccgca
      181 ccgcatgaga actgacctcc atgaccaccc cgaaacagtt ctggtcgcgc atggcgcgga
      241 accaggaatg cagcgtgacg gcgtcgggtg tcgtgtgcgg gtcgcgcacc agttgttcga
      301 cgccgtggcc gagcgtgccg atgattccca tccggcgccc ggcgcgttga aaaatcgatc
      361 gcagcagata cgtggtcgac gtcttgccgt tggtcccggt gacgccgcac aagaacaggt
      421 cgcgcgcggg gtcgccgtag aagcgcgagg ccatcaccgc gagcgcctgc gcggagttgg
      481 cgaccacgac cgcaggaacg cgcgcgctgg ggcatgcgtg ctcgaccacc accgcggaag
      541 caccgcgcgc caccgcgtcg tcgatgaagt cgtgcccgtc gctcgccgta cccggtaccg
      601 cgacgaagac gttgccgggc cggagcgcgc gcgagtcgat cgcgatcccg gtcaccgcga
      661 gtgcgcgcgc gggcgcgtcg agcggtgcca gcgcgagcgg ccccgccgca tcgaccagcg
      721 cgcccaaggt caccgtgcgc gtctccatgg cttcctccgc gaccgtcatc gccgctggcc
      781 cgatccccga gccgtcgcgt tgtgcgcggc cacgactccg gccgcgttcc gctggttcga
      841 cgcgcgcgcg tcccgtgcgc gcggcgcctg ctcgtccccg tcgtcgccgg cacgcaccgc
      901 gtcgcgatag ctcgcgcgca ggcgtcgatt gctatccacc ccggtcttct ccaccccgga
      961 cgcgtccatg acgaacagcc gcacgacgcc gtcgcgatcc atgggcgatc ccggcgccgg
     1021 ctcctgcgcc accacccgcc cgcgctcgcc cgaggcgagc acgttggcgc ccaaccgccg
     1081 cgccgccgac aacgccgcct cgcgatccat gcgcaagaaa ttgggcgccg tggcgccgcg
     1141 cccatcgagc gaggccacgc gcatcgtctc cagcgacagc acgtcgtcga accacgcggt
     1201 ggacgtggcc agtgcgcgac agatgcgcgc gaacacgggt gcggcggaat cgccgccgaa
     1261 gcgcgaggcc cacttgggct cgtcgagcat gacgatggcg gcgatcttgg gcttctcgta
     1321 gggagcgaat ccgacgaagc tcgacacgta gcggtgcagg aggtagccgc ggctccccgc
     1381 cttctgcgcc gtaccggtct tgccggcgac gcgcatgaac tcgaggcgcg cggcctgcgc
     1441 ggtcccgtcc tcgaccacgc gcaggcacat cgcgcgcagc gtggcggcgg tttcctccga
     1501 gaccacgcga cgcacgacca cgggttcgga gcgcgaggta tcgccggtct cgggatcggc
     1561 cacaccgcgc accaagcgcg gcatcatcat gacgccgtcg ttggcgatgg ccgcgaacgc
     1621 gttcagaatc tggatcggcg tcaccgcgac ctcctggccg aaggccatgg tcgccttggt
     1681 gcgcgccgac cagcgctcga tgggcggcac caggccggcc gactcgcccg gcagcccgat
     1741 gccggtcttg gtcccgaagc cgaacaggcg cgcgtagccg tacaagtcgc cgggctgcaa
     1801 atacgacgac gccttcgcca tcacgatgtt gctcgagtac atgaaggcct cgtccagggt
     1861 gagccagcgg tgcgggtgag ggtcgcgaat ggtggcaacg ccgatgtcgg ccttgccgtc
     1921 ttcggcgtcg aaggagtcgg cgggtgtgac gcgcgtcttg tccaagagcg ccgccatcgt
     1981 caccagcttg aaggtcgagc ccggctcgta tacatgcgac acgctgcgca gggtccacag
     2041 cgagtcggcg cgcgaggcgc gctcgcgatc ggcgatcgac ggcagctcgg ccagggccag
     2101 cacgtcgccg gtcgcgatct ccatcacgat caccgcacct ccgcgcgcgt cgaattcttc
     2161 caccgcacgc cgcaactcgg tctcggccac atcctgcacg gtggcgtcga tggtcaggta
     2221 cacgtgcttg ccgtcgaccg gc
//
LOCUS       DASPGK010000183         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_397836, whole genome shotgun sequence.
ACCESSION   DASPGK010000183 DASPGK010000000
VERSION     DASPGK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397836"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="VEC56_05220"
     CDS             <1..1891
                     /locus_tag="VEC56_05220"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXV13587.1"
                     /translation="LTRVAGTRVHLVAQPLPVNAPATGVLVVAHDVSYLDERASQRFA
                     QYAFWILIVTLLAVTLVVGSTWLAYERPLVKLADWMRRLRVDDAVESPPGLPAGRLRS
                     ESDRLAASFRAARSTGRALSEAAVHAEQVWTRDRLRTHALASLGDARELIVVSNREPY
                     MHQRRDGQARMMVPAGGLVTALDPVLEACGGLWVAHGAGDADRETADAAGRITVPPGD
                     ARYTLRRVWLTREEEQGYYYGLSNEGLWPLCHLAHERPIFRVADWDQYVRVNRRFADT
                     VLDEAGASGAVVLVQDYHLALVPRMLKEAHPELRVGIFWHIPWPNPEAFRICPWGVPI
                     LDGMLGADLVGFQLQQYANNFLDTVDRMLESRIDRDHFTVELRGRRTVVQPFPISVQP
                     WAERGVPEGDALEQRKAAFREQHQIGDAFVAVGVDRIDYTKGLPERFRACERLLDQYP
                     QYRGRFTLVQLGAPSRTHIPRYREYLDHLEALADEINWRYQTDGWKPIRLLVGHHDSA
                     TVYTCLHMAPMCIVSSLHDGMNLVAKEYVAAQAGRDGVLVLSEFAGAARELNDALIVN
                     PYDIEQFAEAIRHGMEMEASERVARMKRMYASVETNNVYHWAGNLLSGLARTQPSNVG
                     APAGT"
     gene            1922..4054
                     /locus_tag="VEC56_05225"
     CDS             1922..4054
                     /locus_tag="VEC56_05225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXV13588.1"
                     /translation="MTLTKRLAVAIAIVCLAASPIVGDATERRPISETDILQFQWIGD
                     PQVSPDGSQVVFVRVVVNEDKGRYQTSLFVVPAKGGAPARPFTAGPFDAQPRWSPDGK
                     TIAFVRAIEKDGKPGKGQIHLISAEGGEARALTDLAKGAGSPRWSPDGKTIAFTSATL
                     PEDTTKEDPKESDVRVITRAVYRFNGQGYAEPGRHDHIWTVSSAFDAEGERPTPRQVT
                     SGSFDEENIAWSPDGSRLYFVSTRVLEPYYVPNDADLYSVPAPGGEIVRVASIDGEID
                     AFAPSPDGKQIVFMGTLNASPPRSYNQPDLFVTDATPESTPRNLTADYDFDMGGGVSG
                     DQRAPRGGRGGGIVWSRDGKSVLVTSTALGRTNVKRVRVSDGRMEAFTDGNNDVLAQS
                     ASADGATLVALVSTATDIGDLFVTDARKKAASFKPLTRVNDALFSKLDVPEPEEIWYD
                     SFDGQKIHGWIVKPPGFDPSKKYPMILQVHGGPHIPYGYCFTHEFLWMAAKGYVVLYT
                     NPRGSSSYGQEFGNLIQFAYPGDDYKDLMAGVDFVLQRGYVDEKRIGITGGSGGGVLT
                     NWAITQTDRFAAAVSQRSIADWSSFWYACDFSLFQPVWFRGAPWEQPEDFAARSAITH
                     VAKVTTPLMLIEGEADMRTPPGNGGEQMFRALKYLKKPAVMVRFPDETHELSRSGKPS
                     HRVERLQHIVAWFDQYLQGKDDGAYDVR"
     gene            4060..>4395
                     /locus_tag="VEC56_05230"
     CDS             4060..>4395
                     /locus_tag="VEC56_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXV13589.1"
                     /translation="MPRYVAFLRAINVGGHVVKMDALRRLFEALDFERVETVIASGNV
                     IFDAPSKSAAALEKKIEGHLHKKLGYEVATFLRTPAQMVGIAEYTPFKRAELDAEGNV
                     LYVGFLGEAP"
BASE COUNT          755 a         1407 c         1498 g          735 t
ORIGIN      
        1 gcttacgcgg gtggcgggca cgcgcgtcca cttggttgcg caaccgctgc ccgtcaacgc
       61 ccccgcgacc ggcgtgctcg tcgtcgcgca cgacgtgtcc tacctcgacg agcgtgcgtc
      121 gcagagattc gcgcagtacg cgttctggat cctcatcgtt actctattgg cggtcacgct
      181 cgtggtgggc agtacctggc tcgcgtacga acgcccgctg gtcaaactgg ccgactggat
      241 gcgccgattg cgcgtggatg acgccgtgga atcacccccg ggtctcccgg cgggacggtt
      301 gcggagcgag tcggatcgct tggccgcgtc gttccgggcc gctcgttcga ccggacgagc
      361 cctctcggag gcggctgtgc acgcggagca ggtatggacg cgcgatcgcc tgcgcacgca
      421 cgcgttggcc tcgctgggcg acgcacgaga gctcatcgtg gtgagcaacc gcgagcccta
      481 catgcaccag cggcgcgacg ggcaagcgcg catgatggtt ccggcgggtg gactggtgac
      541 ggcgctggac ccggtcctgg aggcgtgcgg cggactgtgg gttgcgcacg gcgccggcga
      601 cgccgaccgc gaaaccgccg acgccgcggg acgcatcacg gtgccgccgg gtgacgcccg
      661 ctacacgctg cggcgcgtat ggttgacgcg cgaggaggaa cagggctatt actatgggct
      721 ctcgaacgag ggactgtggc cgctatgcca tcttgcgcac gagcgcccga tcttccgcgt
      781 cgcggattgg gatcaatatg tccgggtgaa tcgccggttc gcggacaccg tcctcgacga
      841 ggccggtgcg agcggagcgg ttgttctcgt ccaggactat cacctcgcgc tggtgccgcg
      901 aatgctcaaa gaggcccatc ccgaactgcg cgtggggatt ttctggcaca taccgtggcc
      961 gaatccggag gcgtttcgta tttgtccgtg gggtgtcccg atcctcgacg gtatgctggg
     1021 agcagacttg gtcggattcc agctgcagca gtatgccaac aactttctcg acacggtgga
     1081 ccgtatgctg gaatcgcgca tcgatcggga tcacttcacc gtcgagcttc gcgggcggcg
     1141 tacggtcgtg cagccgtttc cgatcagcgt gcagccgtgg gccgaacgcg gtgtgcccga
     1201 gggtgacgcg ctcgagcagc ggaaggccgc gtttcgcgag cagcaccaga tcggagacgc
     1261 gttcgtcgcc gtcggggtcg atcgtatcga ctacaccaag ggactgccgg aacggttccg
     1321 cgcttgcgag cggctgttgg accagtatcc gcagtatcgg gggcgtttca ccctggtcca
     1381 gctcggcgct ccttcgcgca cgcacatccc gcgttatcgc gagtacctgg atcatctcga
     1441 ggcgttggcg gacgagatca actggaggta ccagaccgac ggctggaagc cgattcgctt
     1501 gctggtgggt caccacgatt ccgcgaccgt gtacacctgc ctccacatgg cacccatgtg
     1561 tatcgtgagc tccttgcacg atggaatgaa cctggtcgcc aaggagtacg tggccgcgca
     1621 agccggtcgc gacggcgtgc tcgtcctgtc cgagttcgcg ggcgcggcgc gcgagctgaa
     1681 cgacgcatta atcgtgaatc cctatgatat tgagcaattc gccgaggcga ttcgccacgg
     1741 catggagatg gaggcctccg agcgagttgc tcgcatgaag cggatgtacg cgagcgtgga
     1801 gacgaataac gtctatcact gggcagggaa tctgctctcc ggattggccc gcacccaacc
     1861 gagcaacgtc ggtgccccgg ccggcacgtg agccgcgcat cgatgcaaca aggagtcgac
     1921 gttgacattg actaagcggc tagctgtcgc aatcgcgatc gtttgtctcg cggcgtcgcc
     1981 gatcgtgggc gacgcgaccg agaggcgtcc catcagcgag accgatatcc tccaattcca
     2041 atggatcggc gacccgcaag tctctcccga cggctcgcag gtggtgttcg tgcgcgtggt
     2101 cgtcaacgaa gacaagggcc gctaccagac gtcgctgttc gtcgtgcccg cgaagggggg
     2161 cgcccccgcg cgcccgttca cggcgggacc gttcgacgcg cagccgcgtt ggtcgcccga
     2221 cggcaagacg atcgcgttcg tgcgcgcgat cgagaaagac ggcaagccgg ggaaggggca
     2281 gatccacctc atatcggccg agggcggcga ggcgcgcgcg ctcaccgatc tcgccaaggg
     2341 tgcgggctca ccgcgctggt cgcccgacgg caagacgatc gcgttcacgt cggccacgct
     2401 ccccgaagac accaccaagg aagaccccaa ggagagcgac gtgcgcgtga tcacgcgcgc
     2461 ggtctatcgc ttcaacgggc agggttacgc cgagccgggg cgccacgacc acatctggac
     2521 ggtgtcgtcg gccttcgacg ccgaaggcga gcgaccgacg ccgcgtcagg tcacgtcggg
     2581 cagcttcgac gaggagaaca tcgcgtggtc gcccgacggt tcgcgcctct acttcgtgtc
     2641 gacgcgcgtc ctcgagccct actacgtgcc caacgacgcc gacctctatt cggttccggc
     2701 gccgggcggg gagatcgtgc gcgtcgcgag catcgacggc gagatcgacg ctttcgcgcc
     2761 ctcgccggac gggaagcaga tcgtgttcat gggcacgctc aacgcgtcgc cgccgcgttc
     2821 gtacaaccag cccgatctgt tcgtaaccga cgcgacgccg gaatcgacgc cgcgcaacct
     2881 gaccgcggac tatgacttcg acatgggtgg aggggtcagc ggcgaccagc gcgcgccgcg
     2941 cggggggcgc gggggcggca tcgtgtggag ccgcgacggc aagtcggtgc tggtgacgag
     3001 caccgcgctg ggtcgtacga acgtgaagcg cgtccgcgtc tccgacgggc gcatggaagc
     3061 gttcaccgac gggaacaacg atgtgctcgc gcaatcggcg agcgccgacg gcgccaccct
     3121 ggtcgcactc gtctcgaccg cgaccgatat cggcgacctg ttcgtcaccg atgcgcgcaa
     3181 gaaggccgcg agtttcaagc cgctcacgcg cgtcaacgac gcgctgtttt cgaagctgga
     3241 cgtcccggag ccggaagaga tttggtacga cagcttcgac ggccagaaga tccacggctg
     3301 gatcgtgaag ccgcccggct tcgacccgtc gaagaagtat ccgatgatcc tccaggtcca
     3361 cggggggccg cacatcccgt acggttactg cttcacgcac gagttcctgt ggatggcggc
     3421 caagggctac gtcgtgctgt acaccaaccc gcgcggcagc tcgtcgtacg gacaggagtt
     3481 cggcaatctg atccagttcg cgtacccggg cgacgattat aaggacctga tggcgggcgt
     3541 cgatttcgta ttgcagcgcg gctacgtcga cgagaagcgc atcgggatta cgggcggcag
     3601 cggcggcggc gtgctgacca actgggccat cacgcagacg gaccggttcg cggccgcggt
     3661 gtcgcagcgc tcgatcgcgg attggtcgag cttctggtac gcgtgcgatt tttcgctctt
     3721 ccagccggtc tggttccgag gcgcgccgtg ggagcaaccc gaggacttcg ccgcgcgctc
     3781 ggcgattacg cacgtcgcca aggtgacgac tccgctcatg ttgatcgaag gcgaagccga
     3841 catgcgcacc ccacccggca acggcggcga gcagatgttc cgcgccctca agtacctgaa
     3901 gaagccggcc gtgatggtgc gtttccccga cgagacgcac gagctttcac gctctggaaa
     3961 gccgtctcac cgcgtcgagc gactccagca catcgtggcc tggttcgacc agtacctgca
     4021 gggtaaggac gacggcgcat acgacgtgcg gtgagtgaaa tgccgcgcta cgtcgcattc
     4081 ctgcgcgcga tcaacgtggg cggccacgtg gtcaagatgg acgcgctgcg ccggctgttc
     4141 gaggcgctcg acttcgagcg cgtcgaaacg gtgattgcca gcggcaacgt gatcttcgac
     4201 gcgccgtcga aaagcgcggc ggcgctcgag aagaagatcg aaggccacct ccacaagaag
     4261 ctcggctacg aagtcgcgac gttcctgcgc acacccgcgc agatggtcgg aatcgcggag
     4321 tatacgccgt tcaagcgcgc cgagctggac gccgagggca acgtgctcta cgtcggcttc
     4381 ctcggcgagg cgccc
//
LOCUS       DASPGK010000184         5421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1450932, whole genome shotgun sequence.
ACCESSION   DASPGK010000184 DASPGK010000000
VERSION     DASPGK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450932"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VEC56_05235"
     CDS             <1..641
                     /locus_tag="VEC56_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13590.1"
                     /translation="GGGFADGTHFNLNFQPVAPIKFEKFTVIARTIVPYNSFPAGGSE
                     RATGIGDIQEQLYVTPPNPGKVVWGVGPMFSFPTATNNFAETGSWAVGPGAVVLTMPG
                     PWVIGGLVNQYWTYSDEGGDPEVNLLVFQPFVNYNFAGGWALAFAPIWTANWDAPDGE
                     EWTIPLGLGLSRTTVFNRRPMVFGVQVYRNVEHPTGGAETQVRAIVSFLYPK"
     gene            846..1790
                     /locus_tag="VEC56_05240"
     CDS             846..1790
                     /locus_tag="VEC56_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV13591.1"
                     /translation="MRTRKKLKRAGEIAVVGALIGVARAVPRRLGSAAFAGIGALAAR
                     AFERDRLRAVENIAVAFPDAPAPIRAALARASFKALGRNVYEALRLSGMSPQSVRERV
                     ERVEGMEHFLDAHRAGKGVIVITGHIGCWELLPAYFVSLGYPVSVVARRMKSERLNAK
                     LVAMRASVGVKSLDRDENPRRMIEPLRRGEILGVLIDQHTRVAGMYVPFFGRPAYTPT
                     AVAKLALATGAAIVPMGIYLGRGGRHVVRVAPAIPIDATLRDSPNKDAAVRAITAECS
                     LAVEQLIRFDPAQWVWFHHRWREEERDEGAQVAYAAQR"
     gene            1774..2340
                     /gene="lptC"
                     /locus_tag="VEC56_05245"
     CDS             1774..2340
                     /gene="lptC"
                     /locus_tag="VEC56_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXV13592.1"
                     /translation="MRRSDRARAALAVALVALHALACDEEAKKVASASAELPDETVSD
                     FRTQESDSGRVQWTVKAPRADRYNARNVFVMDDPKIEFYDEMGNLQTTLTADNGEYSL
                     TTHDMLAYGNVVAVTFKGEVLETDTLRYLNQTDKIVSDSFVKLTRGRDVLTGIGLECD
                     HTLESVDIKKDVRARILEEDGKLDDITP"
     gene            2351..3097
                     /gene="lptB"
                     /locus_tag="VEC56_05250"
     CDS             2351..3097
                     /gene="lptB"
                     /locus_tag="VEC56_05250"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXV13593.1"
                     /translation="MEPVTATARAKLATESVAKSYGRRRVVNGVSIDVNGGEIVGLLG
                     PNGAGKTTTFYMIVGMIRPNEGSIRLDGTDITHMPMYKRARLGIGYLPQEPSIFRGLS
                     VEDNLMAVIETMPYPRSERTAVLDGLLAQLDIAHIRKSMGYQLSGGERRRVEITRALV
                     ARPKFMLLDEPFAGLDPIAVQDLQGIVRKLKEQGLGVLITDHNVRDTLSITDRAYVMY
                     EGSIKRSGTAAELAADTEVREIYLGNAFKL"
     gene            3121..4629
                     /gene="rpoN"
                     /locus_tag="VEC56_05255"
     CDS             3121..4629
                     /gene="rpoN"
                     /locus_tag="VEC56_05255"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXV13594.1"
                     /translation="MEMKIGLHLRQKQQLVMTPKLQQALKLLQMPAIELQQMLKQEIM
                     ENPLLEEVEEYEEIVEETEQHADGSGEGSEGDGDATAPAEVTAPAAEEAPAEEPIEVK
                     TDGGEAGDERVDWDEYFNDGFDIGRAGAEEEHKEEFFEKVPVAKRSFTDQLMGQLRIV
                     TDDPRMLTIGDYLIGSLDESGYLTCDLEEVAHTFSVGVDEVDKVLELIQNFDPPGVGA
                     RNLQECLLLQLKYRGQGDSIAAAIIRDHFDEFKQKKYVDIARKLRISVQDVQDQCKLI
                     STLDPKPGLEVVAEDPKYVIPDLIVDSIDGKYVVYLNDRNIPRLRVSQHYHDELMREV
                     RDGDSEAREFINARLKSAKWLIQTIEQRRRTMVKVMECIVRKQRDFFDKGTAFLRPLT
                     LQQVASEINMHESTVSRVTTNKYVQTPRGVFELKFFFSSSLGTQDGGEVSAKSAKDKI
                     RRIIEAETDRTPLSDQKIADMLKKEGLNIARRTVAKYREQLNILPARMRKQY"
     gene            4768..5145
                     /gene="raiA"
                     /locus_tag="VEC56_05260"
     CDS             4768..5145
                     /gene="raiA"
                     /locus_tag="VEC56_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXV13595.1"
                     /translation="MFLPKFVIEKGVVMNITTTARHYDLAPALKDYAEGKVHSLKKYF
                     DHIVNAHLIFSLEKYRQRVEITLHVNGKDFVTLQESEDMYLSVDRAVEKLEEQLKRYS
                     GKLKKRKRAQGLGEASGSVAEGE"
     gene            5167..>5421
                     /locus_tag="VEC56_05265"
     CDS             5167..>5421
                     /locus_tag="VEC56_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13596.1"
                     /translation="MSKPQKSTLPVRVLLEDTRGVLQLELVNGIDEGPLPITVTDIHR
                     PGLALAGFMQNYLNERIQVLGETEILYLNTRPEDIRREAVA"
BASE COUNT         1052 a         1704 c         1807 g          858 t
ORIGIN      
        1 agggaggcgg cttcgcggac gggacccact tcaatttgaa ctttcaaccc gtcgccccca
       61 tcaagttcga gaagttcacg gtgatcgcgc gcaccatcgt gccgtacaac agcttcccgg
      121 ccggcgggag cgagcgcgcg accggcatcg gcgatatcca ggagcagctc tacgtcacgc
      181 cgccgaaccc gggcaaggtg gtctggggcg tgggcccgat gttctccttc ccgaccgcaa
      241 ccaacaactt cgcggagacg ggcagttggg ccgtcggtcc cggcgcggtc gttttgacca
      301 tgcccgggcc gtgggtcatc ggcgggctcg tgaatcagta ttggacctac tccgacgagg
      361 gcggcgaccc ggaggtcaac ctgctcgtct tccagccgtt cgtgaactac aacttcgccg
      421 gagggtgggc gctcgcgttc gcgccgattt ggaccgcgaa ctgggacgcg cccgacggcg
      481 aagaatggac gattccgctg ggcctcggac tctcgcgcac cacggtgttc aatcgccggc
      541 cgatggtgtt cggggtgcag gtctatcgca acgtcgagca cccgacgggt ggcgccgaga
      601 cgcaggtgcg cgcgatcgtc tccttcctgt atccgaagtg accggttcgg ggcgccggcc
      661 gctccctccc ggtgttcctc gatcttcccc gttcagcccg gtaagtcctc gttgttacgt
      721 ctcttggcac actcgttgct tgacaccgtt ctcggattgt gctaactaat tgcaggtgaa
      781 cggttttgac cgcttttgcg gcggtcgagc ggaaggattc tgagcacgat ccgcgggggg
      841 cggcagtgcg gacgcggaag aaactcaagc gggccggaga aatcgcggtc gtcggggcgc
      901 tgatcggggt cgcgcgcgcg gtgccgcgcc ggctcggttc cgccgccttc gccggcatcg
      961 gcgcactggc cgcccgcgcc ttcgagcgcg accggcttcg cgccgtcgag aatatcgcgg
     1021 tcgcgttccc ggacgcgccc gcgccgatcc gggccgcgct cgcgcgcgcg agtttcaagg
     1081 cgctgggccg caacgtgtac gaagcgctgc gcctctccgg aatgtcgccg caatcggttc
     1141 gcgaacgcgt cgagcgcgtc gaagggatgg aacacttcct cgatgcgcac cgggccggca
     1201 agggcgtcat cgtaatcacg gggcacatcg ggtgttggga actcctgccc gcgtacttcg
     1261 tgagcctcgg ctatccggtc tcggtggtcg cgcggcgcat gaagtccgag cggttgaacg
     1321 cgaagctcgt cgccatgcgc gcgtcggtcg gggtcaagag cctcgaccgc gacgagaacc
     1381 cgcgccgcat gatcgagccg ctgcgacggg gcgagatcct gggcgtgctg atcgatcagc
     1441 acacccgtgt ggcggggatg tacgtgccct tcttcggccg cccggcctac acgccgacgg
     1501 cggtcgcgaa gttggcgctc gcgaccgggg ccgcgatcgt ccccatgggg atctatctcg
     1561 ggcgcggcgg acggcacgtc gtacgcgtgg cccccgcgat cccgatcgac gcgacgctgc
     1621 gcgattcgcc gaacaaggac gccgcggtgc gcgcgatcac ggccgagtgc tcgctggcgg
     1681 tggagcagtt gattcggttc gaccctgcgc agtgggtgtg gttccaccac cggtggcggg
     1741 aagaggaacg ggacgaaggg gcgcaggtgg cgtatgcggc gcagcgatag ggcgcgcgcc
     1801 gcgctggcgg tggcgctggt ggcgctccac gcgctcgcgt gcgacgaaga ggcgaagaag
     1861 gtggcgtcgg cgtcggccga gcttcccgac gagaccgtga gcgactttcg cacccaggag
     1921 tccgacagcg ggcgggtgca gtggacggtc aaggcgccgc gcgccgaccg ctacaacgcg
     1981 cgcaacgtgt tcgtcatgga cgacccgaag atcgagttct acgacgagat ggggaacttg
     2041 cagacgacct tgaccgccga caacggcgag tactcgctca ccacgcacga catgctggcc
     2101 tacggcaacg tcgtcgcagt gacgttcaag ggcgaggtgc tggagacgga cacgctgcgt
     2161 tacctgaacc agaccgacaa gatcgtgagc gacagtttcg tcaagctcac gcgcggccgg
     2221 gacgtgctga ccggcatcgg gctcgagtgc gaccacacgc tcgaatccgt cgatatcaag
     2281 aaggacgtgc gggcccggat tctggaagaa gatggaaagc tcgacgatat caccccctag
     2341 ggtggcaccg atggaaccgg tgaccgccac ggcacgcgcg aaactcgcca ccgagtcggt
     2401 ggcgaagagc tacggccgtc gccgcgtcgt gaacggggtc agcatcgacg tcaacggcgg
     2461 agagatcgtg gggctcttgg ggcccaacgg cgccggcaag acgacgacgt tctacatgat
     2521 cgtcgggatg attcggccca acgaaggatc gattcgcctc gacggcaccg acatcacgca
     2581 catgccgatg tacaagcgcg cgcgcctggg catcggatat ctgccgcagg agccgtcgat
     2641 ctttcgcggt ttgagcgtcg aggacaactt gatggccgtg atcgagacca tgccgtaccc
     2701 gcgctcggag cgaacggcgg tgctcgatgg gctcttggcc cagctcgaca tcgcgcacat
     2761 tcgcaagtcg atgggctacc agctttccgg cggagagcgc cggcgggtcg agatcacccg
     2821 cgcgctggtc gcgcgaccga agttcatgct gctcgacgag cccttcgccg gtctcgaccc
     2881 gatcgcggtc caggacttgc agggcatcgt gaggaagctc aaggaacaag ggctcggcgt
     2941 gctcatcacc gatcacaatg tgcgcgacac gctgagcatc accgaccgcg cctacgtcat
     3001 gtacgaaggc agtatcaagc ggtcgggaac cgcggccgag ctggccgccg acaccgaagt
     3061 gcgcgagatc tatctgggca acgcgttcaa gctctagggt ccacgcgagg cgacgttctt
     3121 atggaaatga aaatcgggct gcacctacga cagaagcaac agctggtgat gacgccgaag
     3181 cttcagcagg cgctgaagct cttgcagatg cccgccatcg agctccagca gatgctcaag
     3241 caggagatca tggagaaccc tctgctcgag gaagtggagg agtacgaaga gatcgtcgag
     3301 gagaccgagc agcacgcgga cggttcggga gaaggcagcg aaggcgacgg cgacgcgacc
     3361 gcgcccgcgg aagtcaccgc cccggcggcc gaggaagccc ccgccgagga gcccatcgag
     3421 gtcaagaccg acggcggcga ggctggcgac gagcgcgtcg actgggacga atacttcaac
     3481 gacggcttcg acatcgggcg cgcgggcgcg gaggaagagc acaaggaaga gttcttcgag
     3541 aaagtcccgg tcgcgaagcg cagcttcacc gaccaattga tggggcagct gcgtatcgtg
     3601 accgacgacc cgcgtatgct gacgatcggc gattatctga tcgggtcgct cgacgagagc
     3661 gggtacctca cctgcgatct ggaagaagtg gcgcacacct tcagcgtggg cgtcgacgaa
     3721 gtcgacaagg tgctggagct gatccagaac ttcgacccgc ccggcgtggg cgcgcgcaac
     3781 ctccaggagt gcctcttgct ccagctcaag taccgcgggc agggcgactc catcgcggcg
     3841 gccatcattc gcgaccactt cgacgaattc aagcagaaga agtacgtcga catcgcgcgc
     3901 aagctgcgca tctcggtcca ggacgtccag gaccagtgca agctcatcag cacgctcgac
     3961 cccaagccgg ggctcgaagt ggtggccgag gaccccaagt acgtcattcc ggatttgatc
     4021 gtcgacagca tcgacggcaa atacgtcgtc tatttgaacg accgcaatat tccgcgcctg
     4081 cgcgtgagcc agcactacca cgacgagctc atgcgcgagg tgcgcgacgg cgacagcgag
     4141 gcgcgcgagt tcatcaacgc gcgcctcaag tcggccaagt ggctgatcca gaccatcgag
     4201 cagcgccggc gcaccatggt caaggtcatg gagtgcatcg tgcgcaagca gcgcgatttc
     4261 ttcgacaagg ggaccgcgtt cctgaggccg ctgacgctgc agcaggtggc gagcgagatc
     4321 aacatgcacg agtcgacggt tagccgtgtg accaccaaca agtacgtgca gacgccgcgc
     4381 ggcgtcttcg agctgaagtt tttcttctcg tccagcctgg ggacgcagga cggcggcgag
     4441 gtctcggcca agagcgccaa ggacaagatc cgccgcatca tcgaggcgga gaccgaccgc
     4501 acgccgctgt cggaccagaa gatcgcggat atgctcaaga aggaagggct gaacatcgcc
     4561 cgtcgcacgg tggcgaagta tcgcgagcag ctcaatatcc tgcccgcgcg tatgcgcaag
     4621 cagtactagc gttttggggg cgttaaggag gcgcttgccg cctccccggg tcgggcgtat
     4681 attcgaagca gcgaaatgat tcacctcgca ccgtcaacga gacgagccat cgcgttaaca
     4741 ccctccccaa gtcggcgtca gaccgccgtg ttcctcccga agttcgtaat cgagaaaggg
     4801 gtcgtcatga acattactac gaccgcacga cactacgact tggcgcccgc cctcaaggac
     4861 tacgccgagg gcaaggtgca cagcctgaag aagtacttcg accatatcgt caacgcgcac
     4921 ctcatcttct cgttggagaa gtaccgccag cgcgtggaga tcacgctcca cgtcaacggc
     4981 aaggacttcg tcacgctgca ggaatccgag gacatgtatc tctccgtcga ccgggcggtg
     5041 gagaagctgg aagaacaact caagcgttat agcggcaagc tcaagaagcg caagcgcgcg
     5101 caaggactcg gcgaagcctc gggcagcgtc gccgaggggg aataggcacc caaaaggacg
     5161 caggccgtga gcaagcccca gaagagcaca cttccggttc gcgttctgct cgaggacacg
     5221 cgcggcgtgc tgcagctcga gcttgtcaac ggcatcgacg agggcccgtt acccatcacc
     5281 gtcaccgaca tccatcgacc cggcctggcg ctggccgggt tcatgcaaaa ctatttgaac
     5341 gagcgcatcc aggtgctcgg cgagaccgag attctgtatc tcaatacccg ccccgaggac
     5401 atccgccgcg aggcggtcgc g
//
LOCUS       DASPGK010000185         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_46770, whole genome shotgun sequence.
ACCESSION   DASPGK010000185 DASPGK010000000
VERSION     DASPGK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46770"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VEC56_05270"
     CDS             <1..325
                     /locus_tag="VEC56_05270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13597.1"
                     /translation="ETFPNVVLEAMASGLAVVSTDVGSVREMAEEGRSALIVPPDDEG
                     ALHAALERVVGDVALRRALGARGREIAEERFRIEAMCRKREAIFEELMEPRAPGAMRL
                     GSAER"
     gene            322..1590
                     /locus_tag="VEC56_05275"
     CDS             322..1590
                     /locus_tag="VEC56_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV13598.1"
                     /translation="MNVLKFVFGHRGAGNLVRRSAQIATRFGPGPERMGARFERFMDI
                     LDEFAIRPTFPITALPMSRNPSFAHRLIGRGAELAVHAWSHVDLSALDLEGQRTHMGR
                     AVELFRSCSVPFTGFRAPYLHWNEDTMRVVSEFQFRYSSNQTVWWDVLDVASMTPQQK
                     EGMQRGLDFYRPVAASAYRVLPFRRDGLVEIPVSLPDDEIPLDRMYIHDADYLGAMWR
                     RILDASHARGELFTLQLHPERIDFFADALRGLLADARARKPGVWIATLDEIAQWWDAK
                     ARNRAQFVREGDRFRVEVSACAGTKVLVRANGSDRVAETAVFTVDSSARPCVGVAPGS
                     SREALAVLADLGYIVEVGERADGYAVHLGRVERHDFAAVDACRRVIDESRRPLVRFGV
                     WPEGRKSALAVTGDIDALTIWDFVQRFRGL"
     gene            1597..2688
                     /locus_tag="VEC56_05280"
     CDS             1597..2688
                     /locus_tag="VEC56_05280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13599.1"
                     /translation="MARRILIVSEDLVQPWDEGIKKFAYSIGTALAPDHPVRMINVDR
                     SGGAASRGGTHAREAGAAPIVDVSGSRTFATPALRREVSSFQPEGIVYVPSPSSTAGS
                     FARSYVLRRHWPRAKLGMVALIPRRHGKAARAFLQGAAPDVLFVPSYASLLHASDLSL
                     RAELLPVGVDVATFRPARDGERSAFRRARGIPEDAFVFLHVGHLSEKRNLRSLATLAG
                     IPGAHVVVVGSTSTPEDAALRRDLEAAGVRVIREVVPVDEFYRLADAYVFPTIDSEGC
                     VEIPLSVMEALASGLPVLARPFGGLRDVLAPGEDVRYFESDDEIRAAAARLMGRPRPV
                     ARSMGAFAWRAIAERIVTRLCDPPGARAS"
     gene            2685..>3585
                     /locus_tag="VEC56_05285"
     CDS             2685..>3585
                     /locus_tag="VEC56_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13600.1"
                     /translation="MNQPAALHTRAEFLRVAARIASPYHVEDLSRLDSVTLHAAGRLL
                     EEQKVSFIPIVARYGPILDATPLSEFVRRDVERFRVERDEFERIRVRLDADRVDAMLF
                     KSTGMSPAFHYQSSNLDVLVPEGRASLARAALVDLGYVELLNVEEPKKFLFRRFVGDG
                     SSFAFHLHEVVGWGVPFLDNAPVWRRARRAPDDTDVIIPGPSEALLITLAHWFYEDKA
                     LSLGNLLLTAHALRSMDCALAEPAAHARGRGWEEGFWGALHVFEAAWTHLFGEGFLDG
                     EQRAELGRAPARYAAVRTRLLPYV"
BASE COUNT          506 a         1205 c         1257 g          617 t
ORIGIN      
        1 ggagacgttc cccaacgtcg tcctcgaagc aatggcgagc ggcctcgcgg tcgtctccac
       61 cgacgtgggc agtgtacgcg agatggcgga ggaaggtcgc agcgcgttga tcgttccgcc
      121 cgacgacgag ggggcgctgc acgccgctct cgagcgcgtg gtgggagacg tcgcgttgcg
      181 ccgggctttg ggcgcacgcg ggcgtgagat cgccgaggag cgattccgca tcgaggcgat
      241 gtgccggaaa cgggaagcga tcttcgaaga gttgatggag ccccgagcgc ccggtgcgat
      301 gcgtctgggg agtgcggagc gatgaacgtc ctcaagttcg tattcggtca ccgcggcgcg
      361 ggcaacctcg tgcgccgcag cgcgcagatc gcgacgcgct tcggtcccgg ccccgagcgc
      421 atgggggcgc gcttcgaacg cttcatggac atcctggacg agttcgcgat tcgccccacg
      481 tttcccatca ccgcgctccc catgagccgc aacccgtcgt tcgcgcaccg attgatcggg
      541 cgcggcgcgg agctcgcggt gcacgcgtgg tcgcacgtcg acctctcggc gctcgacttg
      601 gaaggacagc gcacgcacat gggccgcgcg gtggagttgt tccgctcctg ctcggtgccg
      661 ttcacgggct ttcgcgcccc ctacctgcat tggaacgagg acaccatgcg cgtggtcagc
      721 gaattccagt ttcgctacag ctcgaaccag accgtgtggt gggacgtgct cgacgtcgct
      781 tcgatgacac cgcaacagaa ggaaggcatg cagcgcgggc tcgatttcta ccggccggtc
      841 gcggcctccg cctaccgcgt gctcccgttt cgacgcgacg gcttggtcga gattccggtc
      901 tcgctccccg acgacgagat accgctcgac cgcatgtaca tccacgacgc cgactatctg
      961 ggagcgatgt ggcgccggat cctggacgcc agccatgccc gcggggagtt gttcacgctc
     1021 cagctccacc ccgagcgcat cgacttcttc gcggacgcgc tgcgcgggct cttggccgac
     1081 gcgcgcgcgc gcaagccggg ggtatggatc gccaccctgg acgagatcgc ccagtggtgg
     1141 gacgcgaagg cgcgcaaccg cgcgcagttc gtgcgcgaag gcgatcgctt ccgcgtcgag
     1201 gtgagcgcgt gcgcgggtac caaggtgctc gtgcgtgcca acgggagcga ccgcgtggcc
     1261 gagacggccg tgttcacggt cgactcgagc gcgcgcccct gcgtcggtgt cgcgccgggg
     1321 tcgagccgcg aagcgctcgc ggtgctcgcc gacttgggat acatcgtcga ggtgggcgag
     1381 cgcgccgatg gctacgccgt gcacctgggc cgcgtggagc gccacgactt cgccgccgtc
     1441 gacgcgtgcc ggcgtgtcat cgacgagtca cgccgtccgc tggtgcgctt cggcgtatgg
     1501 cccgaggggc gcaagagtgc cctcgcggtc accggagaca tcgacgcgct caccatctgg
     1561 gacttcgtcc agcggtttcg aggcttgtag caacgcatgg cgcgacgcat cctcatcgta
     1621 tccgaagacc tcgtccaacc gtgggatgag ggcatcaaga agttcgcgta ctcgatcggg
     1681 accgcgctgg cgcccgacca ccccgtccgc atgatcaacg tcgatcgcag cggaggggcg
     1741 gcgtcgcgcg gcggaacgca cgcgcgtgaa gcgggcgcgg cgccgatcgt cgacgtttcg
     1801 ggttcacgca cgttcgccac accggcgctt cggcgcgaag tgtcttcctt ccagcccgag
     1861 gggatcgtgt acgtgccgtc gccgtcgtcc accgcaggta gtttcgcgcg ttcgtacgtg
     1921 ctgcgcaggc actggccgcg cgcaaagctg ggcatggtgg cgctgattcc gcgccgtcac
     1981 ggcaaggctg cgcgcgcgtt cctgcagggt gcggccccgg acgtgctctt cgtcccctcg
     2041 tatgcgtcgc ttttgcacgc gagcgatttg tcgctgcgcg cggagctgct tccggtgggc
     2101 gtcgacgtgg ctacctttcg tcccgcgcgc gacggcgagc gatcggcatt ccggcgcgcg
     2161 cgcggcatcc ccgaggatgc cttcgtcttt ctccacgtcg gacacttgag cgagaaacgc
     2221 aatttgcgtt cgcttgcgac cctcgccggc atccccggag cacacgtggt ggtggtcggg
     2281 agcaccagca cgcccgaaga cgccgcgctg cggcgcgact tggaggccgc gggcgtccgc
     2341 gtgattcgcg aggtggtccc ggtggacgag ttctaccggc tcgcggacgc atacgtcttt
     2401 ccgaccatcg acagcgaagg ctgcgtcgag attccgttgt ccgtgatgga agcgctcgcg
     2461 agcgggttgc cggtgctggc gcgccccttc ggggggctgc gtgatgttct ggcacccggc
     2521 gaggacgtcc gttacttcga aagcgacgat gaaattcgcg ccgccgccgc gcgactcatg
     2581 gggcggcctc gtccggtcgc gcgcagtatg ggcgcattcg cgtggcgcgc gatcgcggag
     2641 cgcatcgtga cccgactgtg cgacccgccg ggagcacgcg cgtcgtgaac cagccggccg
     2701 ccctgcatac gcgcgcggag tttctgcgcg tcgcggcgcg catcgcgagc ccgtatcacg
     2761 tcgaggatct ttcgcgtctc gattcggtca cactgcacgc ggcgggtcgc ctcctggaag
     2821 agcagaaggt ctctttcatt cccatcgtcg cgcgctacgg accgatcctg gacgccacgc
     2881 cgctttcgga attcgtccgc cgggacgtcg aacgcttccg cgtcgagcgt gacgaattcg
     2941 agcgcatccg cgtgcgcctg gacgcggatc gcgtcgacgc gatgctgttc aaatcgaccg
     3001 ggatgtcgcc cgcgttccat taccagtcca gcaacctcga cgtgctggtg cctgaagggc
     3061 gcgcttcgct cgctcgcgcg gcactcgtgg acctgggcta cgtcgagctg ctgaacgtcg
     3121 aagagcccaa gaaattcttg tttcggcgct tcgtcggcga cggctcgagc ttcgcgttcc
     3181 acctgcacga agtggtgggc tggggagtcc cgttcctcga caacgcgccg gtgtggcgcc
     3241 gggcgcggcg cgcgcccgac gacaccgacg tgatcatccc gggaccctcg gaggcgcttt
     3301 tgatcacgct cgcgcactgg ttctacgagg acaaggcgct ctcgctggga aacctgctgc
     3361 tcaccgcgca cgcgctgcgt tcgatggact gcgcgctggc cgaaccggcc gcacacgcgc
     3421 gcggtcgcgg ctgggaggaa ggcttctggg gggcgctgca cgtgttcgag gccgcgtgga
     3481 cgcacctctt cggcgaggga ttcctggacg gggagcagcg cgccgagctt ggtcgcgcgc
     3541 ccgcgcgcta cgccgctgtg cgcacgcgcc tgctgccgta cgtgc
//
LOCUS       DASPGK010000186        11164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1687245, whole genome shotgun sequence.
ACCESSION   DASPGK010000186 DASPGK010000000
VERSION     DASPGK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687245"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VEC56_05290"
     CDS             complement(<1..670)
                     /locus_tag="VEC56_05290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV13601.1"
                     /translation="MSTDRFRRLVDIVGDAMQLPAGERAAFVERACAGDADLLAEARS
                     LLAEAHATSFEAVTARLGARVDRAAESLERAATLPAQVGPYRILELLGEGGMGLVYRA
                     EQTSPIRREVALKMVRGGLRGEHARARFEAERQALAVMDHPHIARIYDAGATDDGTPY
                     FAMELVRGEPITTYCDAHHLDVDARIDLFGKVCRAVQHAHIKGVIHRDLKPSHILVSR
                     VDQHA"
     gene            complement(671..1237)
                     /locus_tag="VEC56_05295"
     CDS             complement(671..1237)
                     /locus_tag="VEC56_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXV13602.1"
                     /translation="MDDNRTRVTHILQNLDPSDPRQIGELVAVVYDELREIAQRALRH
                     ERPGHTLQPTALVNEVYLRLFDEPANAWENRAHFFGVVARAMRQVLVDHARKRHAAKR
                     GGDLERVTLVTDIAEAGDNATDVLELHLALEKLSQNDEALARLVELRFFAGLTLDEAA
                     DAIGVSRRKAAKDWAVARLWLQRELGRG"
     gene            complement(1266..2870)
                     /locus_tag="VEC56_05300"
     CDS             complement(1266..2870)
                     /locus_tag="VEC56_05300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV13603.1"
                     /translation="MKRVQDAAVESDRQVTLVRPDAPAPVGKRTPSSFPSDLFEQARR
                     RLRLLAMFFLVAFSFDVLIFVGVMAFVQDVPRDAVRAAPFQWVNLGAALLSAGVWWAA
                     RSPRISATRLHTIGLMFEVVICLTAAMMTYWQYFLSTGLIPNLTWVPAIVIMFPLIMP
                     GPPRRMLLAAIAAGATGPLSLLILDLAGKIDTGDGSPYAPAIVSSIFAVAFAYMGSRV
                     IYGLGREVAKARAMGSYQLEERLGAGGMGEVWSARHRLLARTAAIKLIRPTLSGAAGV
                     SEQARQRFEREAQAIASLRSPHTVDLFDFGIADDGAFYYAMELLDGFDADALVRRFGP
                     IPAERVVHVLRQVCHSLSEAESRGLVHRDIKPSNIFLCRYGEDHDFVKVLDFGIVKSH
                     DGPEGATALTGENVIHGTPAFMAPEQALGSATIDGRADIYAVGCVAYWLLTGELVFTA
                     DTPTGVLMKHIQSAPAAPSSRTELHVPPPLDHVVLACLAKDPAERPQTARELSRRLAE
                     LEVEALWTEARRREWWEKHQPAGRDL"
     gene            complement(2867..4141)
                     /locus_tag="VEC56_05305"
     CDS             complement(2867..4141)
                     /locus_tag="VEC56_05305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13604.1"
                     /translation="MSTPARTNPGRIAYVASGFPKLTETFILREVMTMERSGIPVSVF
                     SVRPRPGGKLHADALPYLEKTHYAPWFGARHVAALASLAVRRPLGLARGLAFLAQDVF
                     AHARYPSAIAKALGATPKMVLFTREMEREGVVHIHGHFANIPTTFAAFAARVLGISYS
                     FTGHAWDIFVPVNQAGLAAKIANARFVATCTGYNTGVLARFCGTDADRGKIWRNYHGL
                     DLARYVAAGQRDGARIVAGGSLVEKKGLLYLVEAVALLRARGVSLRVELVGEGAQRGA
                     LESAIARHALAQSVVLVGSMPHEHLVERMRTSAMVVLPCIETRGGYMDGIPNLLIESL
                     ALEVPVISTPISGIPELVVDGETGLLVPPRDAAALASAIEALLRDPERARAMGRAGRA
                     RVETMFDLDRNVGELVARFRTIMDEPTRGRMV"
     gene            complement(4138..6003)
                     /gene="asnB"
                     /locus_tag="VEC56_05310"
     CDS             complement(4138..6003)
                     /gene="asnB"
                     /locus_tag="VEC56_05310"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV13605.1"
                     /translation="MCGICGLLGDVDPQHARASVKRMADCMRHRGPDDEGFFDDAPVY
                     LGHRRLSVVDLATGHQPISNEDATIWTAYNGEIYNYPSLRSELETKGHAFRTRSDTEC
                     IVHLYEEMGADFPTKLNGMFAIALWDRARKTLVLARDRLGVKPVYYARLADGALAFAS
                     EIRALLRCPGVDTEIDPVALDSYLALQYIPGPRTIYRGVHKLPAGHTLVANAAGAVVT
                     PFWTLEPAPAPPSFDQACDEFRALFEDAVRIRLMSDVPLGAFLSGGIDSGLVVAAMAK
                     AMDRPVRTFSIGFEADGGYSELPYAATVASHFGTSHQTLTVSALDMQSLLPTVAVALD
                     EPLADPAAIPTFLLSRFAREHVTVALTGEGADELFAGYDRYRLEALRDRTDVIPAAVR
                     RGASALAGPLVGPRGRKAMRAAAMDPAAGFVHLRSVMPREMRRELLRAEVAAQVPSDH
                     LEDRMSAYFARSSGLDAALRADTLEWLPDDLLMKVDKMTMLASLEARVPFLDYRIVEQ
                     VAGFPAEWKYRNGRSKVLLKAVAEKVLPSEIVHRPKHGFRPPIGRWLQGSLRAHLEST
                     LLDPAALCNRWLEPRAVRGVVGRFLKGDERLALPVWVLLCLEIWLRNRDAGETAS"
     gene            complement(6265..7494)
                     /locus_tag="VEC56_05315"
     CDS             complement(6265..7494)
                     /locus_tag="VEC56_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13606.1"
                     /translation="MAIRKFLDYYRNTALFLLNLVVLVVAVNGVLYVLFRVKDARTHV
                     SDAERIARIHNSPELRRVYPDLDAESIHQLMQETWSRSYGYEPFTQFRERPFTGRFVN
                     VSEAGFRLSKNQGPWPPLPENCNVFVFGGSTTFGYGLPDSQTVTSFLQDDLTVRASPR
                     VRVYNFGRGSYVSEQERVLLEELLLGGFVPDLALFIDGLNEFAFPAGPAETERLKAVF
                     GYPAGTLDRWPSIQRLPMTRLARYMRRSATAALTSGPRIDRRNLEPTPAFDDGKYRNP
                     EVIERVIERYFQNKKLIEAIAGAYGVPAVFVWQPIPLYKYDLTYHLFAKSDFGSNFFA
                     HYGYADVARRVASEPPGATFLWCADLQASLKEPLYVDKVHYTARMSRLLATTIADSLL
                     ERGLLPVTAPPSRDVSD"
     gene            complement(7737..10703)
                     /locus_tag="VEC56_05320"
     CDS             complement(7737..10703)
                     /locus_tag="VEC56_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13607.1"
                     /translation="MRKLSIVLSMLVLLVASNAMAGGPLAIFDPATQTPYAYPGLVSM
                     YTDNDPMFSLTGPVSNADADARVADGAAQWTGVATSSLTMVVAGDFASIGLPDINLGN
                     LGLVYGAFNGGGYHVIYDHDGSITASLAGPGVLGFSGPEWATGAALDESFAVLNGATV
                     DPGDAMGLWWQGVFTHEFGHGINLAHTQCNGAVVFFGDDEGPAGCALPYGGLPTIAQI
                     ETMYPFIDPSFLTGSGPDQGTVDLLDDRSSVSDVYPAGAWPLGYGTISGTIYLPNGVT
                     EITGANVIVRNLADPFGDCSSMLSGAFTQGDLGPDGRYTFNGLTPGQTYVVYVDEIVA
                     GGFSTPPSLLPGPEEFYNAPNETGDSDTDDKCASTGITAILDTNQDADIIFNFELNLG
                     DDSSVEVALPFLFNLCGEDYSSVWVGSNGFVTFGMGNTDFTESIADLMSGPPRIAIMW
                     DDLNPAAGGTITAEQVGADFVITWDGVPEFATTNSNSFTLTLRPDNTFNMDYPGLDAT
                     DGLVGRALGNGIGNNPGEVDLSAAAEPITGGPGDAVYELFDPGTNDLSGDNLEWDPCP
                     SFTVVIAPATMGVIYATSGAADGGNLYTIDTSTGAATLIGPIGVPMPGLAINSMGEMW
                     GTARISGDLYRVDAATGQGYFQTTTPGVDFIDCIAFDASDVLYGIGYDPPAFQLRIIN
                     TSNGTTTLVGPTNDVWVGLAFHPTNGTLFASVGGFSPVNPDAIATINIGNGATTVIGT
                     TGLGGPIPDVAFDAYGNMYGLKGGGGGTNNLILVNQTNGSGTVIGPTGYPAMSGMASW
                     IEATVPTLLQAFDAKTDENGVTLQWRVHTTGDDLVGFNVRRRDVGGSYGNVLEVLDPS
                     ARSFDDQTVVPGKSYVYQLEIVSDNERSFFSDEIEVRVDALKLELAQNHPNPFNPTTT
                     IGYTVPTRAKVSVQIYDVTGRLVATLVDETRDAGRYSAVWNGRSASGDPVGSGVYFYR
                     LNVGKESLTRKMVLLK"
     gene            complement(10718..>11164)
                     /locus_tag="VEC56_05325"
     CDS             complement(10718..>11164)
                     /locus_tag="VEC56_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13608.1"
                     /translation="GFDANNLPYTEVTISIQESLKGNLSGNYTFRQFGLTAPRDMGNG
                     RTFLGVSPDGFPRFKQGEDVVVFLFATTSLGFQSTAGLMQGKFNVSGRQLTNEINNLG
                     LFQGVSIDPAQLTPAEQKMLGSNEGPLDSELFKGFVKKAVQNAWFE"
BASE COUNT         1934 a         3655 c         3717 g         1858 t
ORIGIN      
        1 cggcgtgctg gtcgacgcgg gagacgagga tgtgggacgg cttgaggtcg cggtgaatga
       61 cgcccttgat gtgggcgtgc tggacggcgc ggcagacctt cccgaacaaa tcgatgcgcg
      121 catcgacgtc caggtgatgc gcgtcgcaat aggtggtaat gggctcgccg cgcaccagct
      181 ccatcgcgaa gtacggggtg ccgtcgtcgg tggcgccggc gtcgtagatg cgcgcgatgt
      241 gggggtggtc catgacggcg agcgcctgcc gctcggcctc gaagcgcgcg cgcgcgtgtt
      301 cgccgcgcag cccgccgcgc accatcttga gggccacttc gcgccggata ggtgaggtct
      361 gctccgcgcg gtacaccaga cccatgccgc cctccccgag cagctcgagg atgcggtagg
      421 gcccgacctg cgcgggcagc gttgcggcgc gctcgagcga ctcggcggcg cgatcgacgc
      481 gcgcgcccag ccgcgcggtg acggcttcga acgaggtggc gtgtgcctcc gcgagcagtg
      541 agcgcgcctc ggcgaggagg tccgcgtcgc ccgcgcaggc gcgctcgacg aacgcggcgc
      601 gctcccccgc gggcagctgc atggcgtcgc ccacgatgtc gacgagtcgg cggaagcggt
      661 ccgtgctcac tcagccacgt cccagctcgc gctggagcca cagccgcgcg acggcccagt
      721 ccttggccgc cttgcgccgc gacaccccga tcgcgtcggc ggcttcgtcc agcgtgagcc
      781 cggcgaagaa acgcagctcg accaagcgcg cgagggcttc gtcgttctgc gacagcttct
      841 ccagcgccag atgcagctcg aggacgtcgg tggcgttgtc accggcctcg gcgatgtcgg
      901 tgacgagcgt cacgcgctcg agatcgccgc cccgtttggc cgcatgccgc ttgcgcgcgt
      961 ggtcgaccaa cacctgccgc atcgcgcgcg cgaccacccc gaagaaatgg gcgcgatttt
     1021 cccaggcgtt ggctggctcg tcgaacaggc gcaggtacac ctcgttgacg agagcggtgg
     1081 gctgcagcgt gtggccgggg cgctcgtgtc gtagcgctcg ctgcgcgatc tcgcgaagct
     1141 cgtcgtacac gacggcgacc agttcgccta tctgacgagg atcggacggg tcgaggtttt
     1201 gcagaatatg ggtgacgcga gtgcggttgt cgtccactcc ggggtccctt tcgctcctcg
     1261 aatcgttaca ggtcgcgacc ggccggctga tgtttctccc accattcgcg ccggcgcgct
     1321 tcggtccaca gcgcttcgac ctccagctcg gcgagccgcc gcgacaattc gcgcgccgtc
     1381 tgcggtcgct cggcggggtc cttggccagg cacgcgagca cgacatggtc caggggcggc
     1441 ggaacgtgca gctcagtgcg cgacgacggc gcggcgggcg cggactgaat gtgcttcatg
     1501 agcactcccg tcggggtgtc ggcggtaaac acgagctccc ccgtcaggag ccagtacgcc
     1561 acgcatccca ccgcatagat atcggcgcgt ccatcgatgg tcgcgcttcc cagcgcctgc
     1621 tcgggcgcca tgaacgccgg cgtgccgtgg atcacgttct caccggtcag cgccgtcgca
     1681 ccctcgggtc cgtcgtgcga cttcacgatg ccgaagtcca gcaccttgac gaagtcgtgg
     1741 tcctcgccgt agcggcacag gaaaatgttc gacggcttga tgtcgcggtg aaccaaaccg
     1801 cgcgactcgg cttccgagag cgagtggcag acctggcgca gcacgtgcac cacgcgctcg
     1861 gcgggaatgg ggccgaagcg acgcaccagc gcgtcggcgt cgaatccgtc cagcagctcc
     1921 atcgcgtagt agaacgcgcc gtcgtccgcg atgccgaagt cgaacagatc gaccgtgtgc
     1981 ggagagcgga ggctggcgat cgcttgcgct tcccgctcga agcgctggcg cgcctgctcc
     2041 gatacgcccg cggcgccgga gagcgtcggg cgaatgagct tgatggccgc ggtacgcgcg
     2101 agcaagcggt gccgcgcact ccacacttcg cccataccgc ccgcacccag gcgctcttcc
     2161 aactggtagc tccccatcgc gcgcgccttc gccacttcgc gaccgagtcc atagatgaca
     2221 cgcgatccca tgtaggcgaa cgcgaccgca aagatcgaag atacgatcgc aggagcatac
     2281 ggactgccgt cgcccgtgtc gatcttcccg gctaggtcga gtatcagcag ggacagcggt
     2341 ccggtcgcgc ccgcggcgat cgcggcgaga agcatccggc ggggcggacc gggcatgatg
     2401 agcggaaaca tgatgacgat ggcagggacc cacgtgagat tgggaatcag ccccgtgctg
     2461 aggaagtact gccagtacgt catcatcgcg gccgtcaaac agatcacgac ttcgaacatc
     2521 aacccgatcg tgtggagacg agtcgcggag atgcgggggc tcctcgcggc ccaccaaacg
     2581 ccggcggaca agagcgccgc acctaggttg acccattgga acggagcggc ccgcacggcg
     2641 tctcgcggaa cgtcctggac gaacgccatc accccgacga aaatcaagac gtcgaaggag
     2701 aacgccacca agaagaacat ggccaggaga cggagacgcc tgcgagcttg ctcgaagagg
     2761 tcggacggaa acgacgacgg tgtccgcttt ccgaccggcg cgggcgcgtc cggacgcacg
     2821 agcgttacct ggcggtcgga ttcgaccgcg gcgtcttgca cgcgtttcat accatccggc
     2881 ctcgcgtagg ttcgtccatg atagtgcgga atcgggccac caattctccc acgttgcgat
     2941 cgaggtcgaa catggtctcg acgcgcgcgc gtcctgcgcg gcccattgcg cgcgcccgct
     3001 ccggatcgcg cagcagcgcc tcgatcgcgc tcgcgagcgc cgcggcgtcg cggggcggga
     3061 ccaagagacc cgtctccccg tcgacgacca gctccgggat ccccgagatc ggcgtcgaaa
     3121 tcacgggcac ttcgagcgcc aggctctcga tgagaaggtt gggtatgccg tccatgtaac
     3181 cgccccgcgt ctcgatacac ggtagcacga ccatggccga cgttcgcatg cgttccacga
     3241 ggtgttcgtg cggcatcgat ccgaccagta ccaccgactg ggccagggcg tggcgcgcga
     3301 tcgcgctctc caaggcaccg cgctgcgctc cctctccgac cagctcgacg cgcaacgaga
     3361 caccgcgcgc acgcagcagc gccaccgctt ccacgagata caggagaccc ttcttctcga
     3421 ccaggctccc gcccgccacg atgcgggcac cgtcgcgttg cccggcggcg acgtaccgcg
     3481 cgagatcgag gccgtggtag ttacgccaaa tcttgccgcg gtcggcgtcg gtcccgcaga
     3541 agcgcgcgag cacgcccgtg ttgtatccgg tgcacgtcgc gacgaagcgc gcgttcgcga
     3601 tcttcgccgc cagacccgcc tggttgaccg ggacgaagat atcccacgcg tgtccggtga
     3661 agctgtagga aatgcccagc acgcgggctg cgaaggcggc gaacgtggtg gggatgttcg
     3721 cgaaatgccc gtgaatgtgg accacgcctt cgcgttccat ttcgcgcgtg aacagcacca
     3781 tcttgggcgt tgctcccagc gccttcgcga tcgcggacgg gtagcgcgcg tgggcgaaga
     3841 cgtcctgcgc gaggaaggcc aagcctcgcg ccaaccccag cggcctgcgc accgcgagtg
     3901 aggccaacgc ggccacgtgg cgcgcgccga accacggcgc atagtgggtc ttttccaggt
     3961 agggcaacgc gtccgcgtgc agttttcctc caggacgcgg tcgcacgctg aacacggaca
     4021 ccgggatgcc gctgcgctcc atcgtcatga cttcgcgcag gatgaacgtc tccgtgagct
     4081 tggggaagcc ggacgccacg taggcgatgc gtcccggatt ggtccgcgcc ggcgtgctca
     4141 cgatgccgtc tccccggcgt cccggttgcg cagccagatc tccaagcaca acaggaccca
     4201 caccggcagt gcgagtcgct cgtcgccctt caggaaacgg ccgacgacac cgcggaccgc
     4261 gcgcggctcg agccagcggt tgcacagcgc ggccggatcc agcagcgtgg actcgaggtg
     4321 cgcgcgcaag ctcccttgca gccagcgacc gatgggtggc cggaatccgt gcttgggacg
     4381 atgaacgatc tcggacggga gcaccttctc cgccaccgcc ttgagcagca ccttgctgcg
     4441 tccgttgcgg tacttccact ccgccggaaa tcccgccacc tgttcgacga ttcgatagtc
     4501 gagaaacgga acccgcgcct cgagggacgc gagcatcgtc atcttgtcca ctttcatcag
     4561 caagtcgtcg ggcagccact ccagcgtgtc cgcgcgaagc gcggcgtcga gaccgctcga
     4621 cctggcgaag taggccgaca tgcggtcctc gaggtgatcg ctcggtacct gcgccgccac
     4681 ctcggcgcgc agcaattcgc gccgcatctc gcgcggcatc acggagcgca ggtgcacgaa
     4741 gccggcggcc ggatccatcg cggcggcgcg catcgccttg cgaccgcggg ggccgaccaa
     4801 cggacctgcc agcgcgctcg caccgcggcg caccgcggcc gggatcacgt cggtgcgatc
     4861 gcgcagcgct tcgagccggt agcggtcgta gccggcgaag agctcgtcgg cgccttcgcc
     4921 tgtgagggcc acggtcacgt gctcgcgcgc gaagcgcgac agcaagaaag tcgggatcgc
     4981 ggcggggtcc gcgagcggtt cgtcgagcgc gacggccacg gtagggagga gcgactgcat
     5041 gtcgagcgcc gacacggtca gcgtctggtg gctggtcccg aagtggctcg ccaccgtcgc
     5101 ggcatacgga agctcggagt acccgccgtc ggcctcgaag ccgatcgaga acgtgcgcac
     5161 cggccggtcc atcgccttcg ccatggccgc gaccacgagg cccgagtcga ttccccccga
     5221 aagaaacgcg cccagcggaa catcgctcat caatcgaatg cgcaccgcgt cctcgaacag
     5281 cgcgcggaac tcgtcgcatg cctgatcgaa cgacggcggc gccggcgccg gctcgagcgt
     5341 ccaaaacggc gtcacgacgg ccccggccgc gttcgcgacc agcgtgtgtc cggccggcag
     5401 cttgtgcacg ccgcgataga tcgtgcgcgg tccggggatg tactggagcg ccaggtagct
     5461 gtccaacgca accgggtcga tctcggtgtc gacgccggga caccggagca gcgcccggat
     5521 ctccgacgcg aaggcgagcg ctccgtccgc gaggcgcgcg tagtacaccg gcttcacgcc
     5581 cagccggtcg cgcgcgagca cgagcgtctt gcgcgcgcgg tcccacagcg cgatggcgaa
     5641 catcccgttg agcttcgtgg gaaaatcggc gcccatctct tcgtagagat ggacgatgca
     5701 ttcggtgtcc gaccgcgtcc ggaacgcgtg gcccttcgtc tcgagctcgg agcgaaggga
     5761 cgggtagttg tagatctccc cgttgtaggc ggtccaaata gtcgcgtctt cgttggagat
     5821 cggctgatgg ccggtggcga gatcgacgac ggaaagccgg cgatgcccga ggtacacggg
     5881 ggcatcgtcg aagaacccct cgtcgtcggg gccgcgatgg cgcatgcaat cggccatgcg
     5941 cttcaccgac gcgcgggcgt gttgcggatc gacgtccccc agtagtccgc agatcccgca
     6001 catgcccgga gattaccaga cagtgcgcgt ttcaaacagg acgggccggc gacccttgca
     6061 tgggtgccga cccgtcgaat gcgccgacga gagtggccgc aatttaccct cgccgacgtg
     6121 tcgcgcctcc attgggtgcg actccgaacc ttcgaaggct aatatagcac agattcggag
     6181 tgatttctcc tattatttcc cggtgggcaa gaaataattc caccggtcgc gcctgaagcg
     6241 cgtaagtgta ataatcccag attgttaatc gcttacgtcg cggctcggag gggccgttac
     6301 gggaagtagt ccgcgctcga gcaggctgtc ggcgatggtc gtcgccagca agcggctcat
     6361 gcgggccgtg tagtgcacct tgtcgacgta gagcggttcc ttcagcgacg cctggaggtc
     6421 cgcgcaccat agaaacgtcg cccccggggg ttccgacgcg actcggcgcg cgacatcggc
     6481 gtacccgtag tgcgcgaaga agttcgatcc gaagtcgctc ttcgcgaaca ggtggtaggt
     6541 caggtcgtac ttatagagcg gaatcggctg ccagacgaaa acggcgggca cgccgtatgc
     6601 gcccgcgatc gcttcgatca gcttcttgtt ctggaagtag cgttcgatca cgcgctcgat
     6661 cacttcggga ttgcgatact tcccgtcatc gaaggccggc gtcggttcga ggttgcgacg
     6721 gtcgatgcgc gggccgctcg tcaacgcggc ggtggcgctc cggcgcatgt agcgcgccaa
     6781 gcgggtcatc gggagccgct ggatcgacgg ccagcgatcg agggttcccg ccgggtagcc
     6841 gaacacggct ttcaggcgct ccgtctccgc cggtccggct ggaaacgcga actcattcag
     6901 cccgtcgatg aagagtgcca gatcgggaac gaatcccccg aggagcaact cctcgaggag
     6961 cacacgctcc tgctccgaca catagctgcc gcggccgaag ttgtacacgc gcacgcgcgg
     7021 cgaggcgcgc acggtgagat cgtcttggag gaaggacgtc accgtctgcg aatccggcag
     7081 gccgtacccg aaggtcgtcg agccgccgaa cacgaagacg ttgcaattct cggggagcgg
     7141 tggccatggg ccctggttct tcgacaaccg gaagcccgct tcgctcacgt tgacgaagcg
     7201 acctgtgaac gggcgctccc gaaactgcgt gaacggctcg tagccgtacg agcgggacca
     7261 cgtttcctgc atcagctggt ggatgctctc cgcgtcgagg tcggggtaga cgcgccgcag
     7321 ctcggggctg ttgtgaatac gcgcgattcg ctccgcgtcg ctgacgtgcg tgcgcgcgtc
     7381 cttgacgcga aacaggacgt agagcacgcc gttgacggcc acgacgagga cgaccagatt
     7441 caacaggaac agggccgtgt tccggtagta gtccaggaac ttccggattg ccacggaagc
     7501 cccctttcgc gcgcccttcg cttcgcccgg agacgtggga gtcgaattct cgggggcgcg
     7561 acgacgccgc gcccgttcga gaggatcggc cagaccggga gtttaccaca tgatcgcggc
     7621 gcggtgaacg ggtccggtac ggctggggtc ttcgccggct ggggaagaaa aagcgccgtg
     7681 gccgaagcgt ctcgctccgg ccacggcgct ggatcatccg cgttgggcgc gaacgactac
     7741 ttcaggagca ccatctttcg cgtgagggat tccttcccca cgttcagacg gtagaagtaa
     7801 acgccgcttc ccaccgggtc gccgctcgcc gagcgaccgt tccacaccgc cgagtagcgg
     7861 ccggcgtcac gggtctcgtc gacgagtgtc gccaccaggc ggccggtcac gtcgtaaatc
     7921 tggacgctga ccttggcccg cgtgggaacc gtgtaaccga ttgtcgtcgt cgggttgaac
     7981 gggttggggt ggttctgcgc cagctcgagc ttgagtgcat cgacacgcac ctcgatctcg
     8041 tctgagaaga acgaccgctc gttgtccgac acgatctcga gttggtacac gtacgacttg
     8101 cccggcacga ccgtctggtc gtcgaagctg cgcgcactcg gatcgagaac ttcgagtacg
     8161 ttgccgtaag aaccgcccac gtcgcggcgg cgaacgttga agccgacgag atcgtcaccc
     8221 gtggtgtgta cacgccactg caacgtcacg ccgttttcgt ccgtcttcgc atcgaaggct
     8281 tgcagcaagg tcggaaccgt agcctcgatc cacgacgcca taccgctcat cgccgggtaa
     8341 ccggtgggtc cgatgaccgt tcccgacccg ttggtctggt tgacgaggat gaggttgttg
     8401 gttccgccac cgccgccctt gaggccgtac atgttgccgt acgcgtcgaa cgcgacgtcc
     8461 ggaatcggtc cgccaagccc ggtggtaccg attaccgtgg tggcaccgtt gccgatgttg
     8521 atggttgcga tcgcgtccgg gttgacgggc gagaaaccgc ccaccgacgc gaacagcgtt
     8581 ccgttggtcg ggtggaacgc cagtccgacc cacacgtcgt tggtcgggcc gaccagtgtg
     8641 gtcgttccgt tcgacgtgtt gatgatgcgc aactggaatg ccggcgggtc gtagccgatg
     8701 ccgtacaaca catcggacgc atcgaacgcg atacagtcga tgaagtcgac gcccggcgtc
     8761 gtagtctgga agtatccctg acccgtcgcc gcgtcgaccc gatagaggtc gccagagatg
     8821 cgagccgtac cccacatctc acccatcgag ttgatcgcga gtcccggcat gggcacgccg
     8881 atggggccga tcaatgtcgc agcacccgtc gaggtgtcga tcgtgtacag attcccaccg
     8941 tcggccgcac ccgacgtggc gtagatcacg cccatcgtcg ccggcgctat gaccacggtg
     9001 aagcttgggc acggatccca ctcgaggttg tcgcccgaga ggtcgttcgt cccgggatcg
     9061 aagagctcgt acacggcgtc gcccggaccg ccggtgatgg gctcggccgc cgcgctcaag
     9121 tcgacctcgc cgggattgtt cccgattccg ttgccgaggg cccggccgac caacccgtcg
     9181 gtcgcgtcga ggcccggata gtccatgttg aaggtgttat ccgggcgcag cgtcagcgtg
     9241 aacgaattgc tgttggtcgt cgcgaactcc ggtactccgt cccacgtgat gacgaaatcg
     9301 gctcccactt gctcggccgt gatggttccg ccggccgcgg gattgagatc gtcccacatg
     9361 atggcgatgc gcggcggtcc gctcatcagg tcggcgatcg actcggtgaa gtcggtgttg
     9421 cccatgccaa acgtcacgaa tccgttcgag ccgacccaca ccgagctgta gtcctcgccg
     9481 cacagattga acaagaacgg aagcgccact tcgaccgacg agtcgtcccc aaggttcaac
     9541 tcgaagttga agatgatgtc cgcgtcttga ttcgtgtcga ggatcgcggt gatgccggtc
     9601 gaggcacact tgtcgtcggt gtcagagtcg ccagtttcgt tgggggcgtt gtagaactcc
     9661 tccggccccg gcaacagcga gggcggcgtg ctgaaaccac ccgccacgat ctcgtccacg
     9721 tacacgacgt aggtctgccc tggcgtcaag ccgttgaacg tgtaacggcc gtcgggcccg
     9781 aggtcgccct gcgtgaaggc acccgagagc attgacgaac aatcgccgaa cggatcggcc
     9841 aggttacgaa cgatgacgtt ggcgccggtg atctcggtga caccgttggg aaggtagatg
     9901 gtgccggaga tggtgccgta gcccagcggc caggcgccgg cgggatacac gtcggagacc
     9961 gacgagcgat cgtcgagcag atcgacggtg ccctggtccg gccccgatcc cgtcaggaac
    10021 gacggatcga tgaacggata catggtttcg atctgagcga tggtgggcaa tccgccgtac
    10081 ggcagcgcgc acccggccgg gccttcgtcg tcaccgaaga acacgaccgc gccgttgcac
    10141 tgcgtgtgcg ccaggttgat gccgtgtcca aattcgtgtg tgaacacgcc ctgccaccaa
    10201 agtcccatcg cgtcgcccgg gtccacggtc gcgccgttga gcaccgcgaa cgactcgtcg
    10261 agcgccgcgc ccgtcgccca ctccggaccg gagaagccga ggacgccggg gccggccaag
    10321 cttgcggtga tggaaccatc gtgatcgtag atcacgtggt atccgccgcc gttgaacgcg
    10381 ccgtagacga gcccgaggtt gcccaagttg atatccggta gcccgatgga cgcgaagtca
    10441 cccgccacaa ccatggtgag tgacgaggtt gcgacaccgg tccactgcgc ggcgccgtcg
    10501 gccacgcgcg cgtcggcgtc cgcgttggaa accggccccg tcaacgagaa catcggatcg
    10561 ttatccgtgt acatgctgac cagccccgga tacgcgtacg gagtttgcgt cgcagggtcg
    10621 aagattgcta gcggtccgcc tgccatcgcg ttggatgcga cgaggaggac cagcatgctc
    10681 aatacaatag atagtttgcg catgtgtgtt cctcctgtta ctcgaaccaa gcgttttgaa
    10741 cggccttctt gacgaacccc ttgaacagct cgctgtcgag cggtccttca ttggaaccga
    10801 gcatcttctg ctcggccggg gtcagctggg cggggtcgat gctgacgccc tgaaagagac
    10861 cgaggttgtt gatttcgttc gtcagctgtc gcccgctgac gttgaacttg ccctgcatga
    10921 gtcccgccgt cgactggaag cccagcgacg tggtcgcaaa caggaacacg acgacgtctt
    10981 cgccctgctt gaagcggggg aatccgtcgg gcgatacgcc gagaaatgtc cggccgttgc
    11041 ccatgtcacg cggagcggtg agcccgaact ggcggaaggt gtagtttccg ctcagattgc
    11101 ccttgaggct ctcctggatc gagatcgtga cttcggtata aggcaggttg ttggcgtcga
    11161 agcc
//
LOCUS       DASPGK010000187         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_750498, whole genome shotgun sequence.
ACCESSION   DASPGK010000187 DASPGK010000000
VERSION     DASPGK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750498"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VEC56_05330"
     CDS             complement(<1..648)
                     /locus_tag="VEC56_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13609.1"
                     /translation="MRKAYSTRNQTLGILNGMLVNLGNAFVDPFTVLPVFITTFGGST
                     VLVGFVSAAFFAGWFLPQVFVANLAQSRRRVLSIYAAAAAFRFVGFAGAGASVFLLDV
                     SQRDLILLCVCAGLSLNALAAGVAAVPFLEITSKTVPVDRRGAFFAGRRVVGGILGIV
                     AGLVIAAVLGGEPGAMWAQSGIYQSVKSAVDAIGWSGYEFPHDYGILIILGAVISA"
     gene            716..1090
                     /gene="acpS"
                     /locus_tag="VEC56_05335"
     CDS             716..1090
                     /gene="acpS"
                     /locus_tag="VEC56_05335"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXV13610.1"
                     /translation="MIRGLGIDSIELARVARVHAEYGARFLERILTPAERDYVARWAD
                     PVPRIAGRFAVKEACMKALGTGWGAGVRWRDIEVVRHPSGKPEIQLHGRAKEICAALG
                     GAVVHCTITHTDDHAMAVVVIE"
     gene            complement(1121..2146)
                     /locus_tag="VEC56_05340"
     CDS             complement(1121..2146)
                     /locus_tag="VEC56_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HXV13611.1"
                     /translation="MKLSIEPIRIETAHPFQIARGTRREYEVFIVTLETDGLLGMGEA
                     APQSFYGENPMSVRGAIQSVGRLLDVDPETARANLNTAGSDLFEVLRPHASVRAALDM
                     ALWDLRGRREGQPVWKLVGADAARAPLTSFTIGFDQPKVIDAKVDAAAPYRILKVKVG
                     LPGDLEILDRVIARGGKLVRVDANEGWDLETAIEKTVELYRRRVEFCEQPLPHDDEEG
                     LRQLKRVSPLPIVLDESIVDPEDVAPRHDQGHGINIKLMKCGGITRALALVEAARAHD
                     MSVMIGCMIETSLGITAAAHVSPLCDYADLDGNLLLASDPFAGVRVEDGRLVLPDEPG
                     LGVRRVG"
     gene            complement(2163..>3069)
                     /locus_tag="VEC56_05345"
     CDS             complement(2163..>3069)
                     /locus_tag="VEC56_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HXV13612.1"
                     /translation="VQDAIGFGGDAPVVATIEQALARRPEVAVVGTAPAGGELEPVMR
                     AHVASCLEAGLDVASGMHAFLADDPALASIARESGARIWDIRRVPEVRAVSNGAGCTT
                     GAKVVLTVGTDCNVGKMTVAVELERAARANGVRSSWAATGQTGILLRGRGVPVDRVVA
                     DFVGGATQALVDLEGRDVDLVVVEGQGSITHPGYAGVTLGILYGAMPDAMVLVHTAGR
                     ERYKRFDHTIPPLSEVVALYESLMRPYTGARVAAIALNTRRLPHDDARRALERASADT
                     GLPAGDLVRFGAEPVWSAVRASIGM"
BASE COUNT          500 a         1093 c         1027 g          449 t
ORIGIN      
        1 agcggaaatg accgcgccca ggatgatgag gatgccgtag tcgtgcggga actcgtatcc
       61 cgaccagccg atcgcgtcga ccgcggattt cacggattga tagatgccgg attgcgccca
      121 catggcgccc ggttcgcccc cgagcacggc cgcgatcacc aggcccgcca cgattcccag
      181 gatcccgccc accacgcgtc ggcccgcgaa gaacgccccg cgccggtcga ccgggaccgt
      241 cttcgacgtg atctcgagga agggtacggc cgcgacgccg gcggcgagcg cgttcaggct
      301 cagccccgcg cacacgcaca acagaatgag atcgcgctga gatacgtcca gcaggaacac
      361 gctggccccc gcacccgcga agcccacgaa gcggaaggcc gccgccgcgg catagatcga
      421 cagcacgcgc cggcgcgact gcgccaggtt ggccacgaac acctgcggca ggaaccaccc
      481 cgcgaaaaac gccgccgaaa cgaagccgac cagcaccgtc gagccgccga aagtggtgat
      541 gaacaccggc agcaccgtga acgggtcgac gaaggcgttg ccgaggttga ccagcatccc
      601 gttgaggatg ccgagggttt ggttgcgagt tgagtacgct tttctcacga ggcgccacgc
      661 taacgcggaa cgggcgcggt agcaagcggc gagtcggctc ggctatactg gcgcgatgat
      721 tcgcggactc gggatcgatt cgatcgagct agcgcgcgtg gcccgcgtgc acgcggagta
      781 cggcgcgcgc ttcctggagc gcatcctgac acccgccgag cgcgactacg tcgcgcgctg
      841 ggccgacccg gtgccgcgca tcgccgggcg cttcgcggtc aaagaggcgt gcatgaaggc
      901 gctggggacg gggtggggtg cgggggtgcg ctggcgcgac atcgaggtgg tgcgccaccc
      961 gagcggcaag ccggaaatcc agctccacgg ccgcgcgaag gagatctgcg ccgcgctcgg
     1021 tggagccgtc gtccactgca cgatcacgca caccgacgac cacgcgatgg cggtggtagt
     1081 gatcgagtga cccgagggct cttcggcgcg actccctcgg ctaccccacc cgtcgaaccc
     1141 ccagacccgg ctcatcgggc aacaccaacc ttccgtcctc gacccgaact cctgcgaacg
     1201 ggtcgctcgc cagcagcagg ttgccgtcga gatccgcgta atcgcacagc ggcgagacgt
     1261 gcgcggcggc ggtgatgccc agcgaggtct cgatcatgca cccgatcatc accgacatgt
     1321 cgtgggcgcg ggcagcctcg accagtgcca gcgcgcgcgt gatacccccg cacttcatga
     1381 gcttgatgtt gatgccgtgg ccctggtcgt ggcgcggtgc gacgtcttcg gggtcgacga
     1441 tgctctcgtc gagcacgatg ggcagcgggc tcacgcgctt caattgacgc agcccctcct
     1501 cgtcgtcgtg cgggagcggc tgctcgcaga actcgacccg gcggcggtac aactccaccg
     1561 tcttttcgat cgcggtctcg aggtcccagc cctcgttggc gtcgacgcgc accaatttgc
     1621 cgccgcgcgc gatcacgcgg tccaggatct ccaagtcgcc cgggagcccc accttcacct
     1681 tgagtatccg gtacggcgcg gccgcgtcca ctttggcgtc gatcaccttc ggttggtcga
     1741 agccgatcgt gaacgacgtc agcggcgcgc gcgcggcgtc ggccccgacg agtttccaaa
     1801 ccggctgccc ttcgcggcgg ccgcgcaggt cccacagcgc catgtcgagt gcggcgcgca
     1861 ccgacgcgtg cggccgcaac acctcgaaga ggtcgctgcc cgcggtgttg agattcgcgc
     1921 gcgccgtctc cggatcgacg tcgagcaggc ggccgaccga ttgtatggcg ccgcgcacgc
     1981 tcatcgggtt ctcgccgtag aacgactgcg gcgccgcctc ccccattccg aggagtccgt
     2041 cggtctccaa cgtgacgatg aatacctcgt attccctgcg cgtgccgcgc gcaatctgga
     2101 acgggtgcgc ggtctcgatg cgaatcggtt cgatggatag tttcacaacg ttccctcttg
     2161 gctcacattc cgatcgacgc gcgcaccgcg gaccacaccg gctccgcccc gaaacgaacc
     2221 agatcgcccg cgggaagacc ggtgtcggcg ctcgcgcgct cgagcgcgcg ccgcgcgtcg
     2281 tcgtgcggga ggcggcgcgt gttgagcgct atggccgcca ctcgcgcgcc cgtatacggt
     2341 cgcatcaaag attcgtacag cgcaaccact tcggaaagcg gcggaatggt atgatcgaag
     2401 cgcttgtaac gctctctccc ggccgtgtgc acgagcacca tcgcgtcggg catggcgccg
     2461 tacaggattc cgagggtgac gcccgcgtat ccggggtggg tgatggatcc ctggccctcg
     2521 accaccacga ggtcgacatc gcgcccttcc aggtcgacca acgcctgggt cgcgccgccc
     2581 acgaagtccg cgaccacgcg gtcgacgggg acgccgcggc cgcgcagcag gatgccggtc
     2641 tgtccggtgg cggcccagct cgagcgcacg ccattcgcgc gtgccgcgcg ctccaactcc
     2701 accgcaaccg tcatcttgcc cacgttgcaa tcggttccca cggtgagcac caccttggcg
     2761 ccggtcgtac agcccgcgcc gttggacacg gcgcgcactt ccggtacgcg ccggatgtcc
     2821 cagatgcgcg cccccgactc gcgcgcgatg gaggcgagcg cggggtcgtc cgcgaggaag
     2881 gcgtgcatcc cgctcgcgac gtcgagaccg gcctcgaggc agctcgccac gtgcgcgcgc
     2941 atgaccggct cgagctcgcc acccgcgggt gcggtgccga cgacggcgac ctcggggcgc
     3001 cgcgcgagcg cttgctcgat ggtggcgacc acgggcgcgt ccccgccgaa gccgatcgca
     3061 tcctgcacg
//
LOCUS       DASPGK010000188         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_399825, whole genome shotgun sequence.
ACCESSION   DASPGK010000188 DASPGK010000000
VERSION     DASPGK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399825"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEC56_05350"
     CDS             complement(<1..706)
                     /locus_tag="VEC56_05350"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV13613.1"
                     /translation="MRKRIDRPAAAWAFYDWANSAFSTTVMAGFFPLLFKQYWCADVD
                     AAVSTHRLGIANSLSSLGIALVAPALGAIADAGGSRKRFLLASAAIGIAATTLLFFVG
                     SGAWAAAATLYVVAAIGFSASMIFYDSLLIDVSTPETSDYVSSYGFALGYLGGGLLFA
                     VNILMYRMPEQFGLSDATSAARWSFLTVAAWWALFSIPLARVVQERKRDGARGWRPAG
                     ARLGEESSAQARTTSPE"
     gene            807..2771
                     /gene="acs"
                     /locus_tag="VEC56_05355"
     CDS             807..2771
                     /gene="acs"
                     /locus_tag="VEC56_05355"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXV13614.1"
                     /translation="MPGIDMKQLDTVLKEARRFKPAESFRKGAHLRSEAEYRKLYKES
                     IQNPRKFWARVASELHWFKKWNLVLDEKKAPFYRWFVGGKTNLAYNCLDRHLDSPTRH
                     KAAIVWEGEPGDRRVLTYADLARDVGTFANALKSLGIKKGDRVAIYLPMTPELVVSLL
                     ACARIGAVHSVIFAGFSAESIRDRVQDCQAKLVITGDGGWRRGKVLPLKDIVDEALAD
                     APSVENVIVVRRNPDSPFPCHMRDGRDQWYHEITDGMSADCPAEKMDSEDMLFLLYTS
                     GTTGKPKGIIHTTGGYMVFTYLTAKYVFDLKPEDMFWCTADIGWVTGHSYIVYGPLQN
                     GATCLLYEGAPDWPNRGRFWEMIQRYRVNVFYTAPTAIRAFMKWGDEWPAKHDLSSLR
                     LLGSVGEPINPEAWMWYQKAIGGKKCPIVDTWWQTETGGIMITPLPGVTDTKPGSATR
                     PFFGVDAAILNDAGQEVDAGYLAIRKPWPGMLRGVYGDPERYKQTYWSKWPGVYFPGD
                     GARKDKDGYYWIVGRVDDVVNVAGHRIGTAELESIFVEHPRVAESAVIGVAHELKGQA
                     LVAFVSLRDGVMGTEALDKELKEWVVKKIGKFAIPDRIVFAGDLPKTRSGKIMRRLLR
                     DVAEGRALGNVTTLADAGVVEKLKSQYEED"
     gene            2799..3824
                     /locus_tag="VEC56_05360"
     CDS             2799..3824
                     /locus_tag="VEC56_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXV13615.1"
                     /translation="MKTAFHVDEIYLRHRNPEGHPERGARLEALLELAKHADRLGVDV
                     IPSRRRATVEELSRVHTPEYVETIASTAGRSLMLDPDTFTVADSYEVACHAAGSALDL
                     VDRVIAREFSNAFAAVRPPGHHAERERAMGFCLFNNVAAAAAHAVQRHGMERVLIVDW
                     DVHHGNGTQDIFWSDPRVLFVSLHQFPFYPGSGHVEEVGEDAGRGFTVNIPMPGGFGD
                     DEWLAAFRRVVEPVAHQFAPELVLVSAGFDAHANDPLGGMRVSERGFAAMADSLLTIA
                     REHADGRLIAVLEGGYDIQALSTSVEAVLDRLTNSGPAAPSAAADGRFGEVFARMQAA
                     QSAYWKL"
BASE COUNT          729 a         1334 c         1337 g          616 t
ORIGIN      
        1 actccgggct cgtcgtccgc gcctgcgcac tagactcctc gcccaggcgg gcgcccgccg
       61 gacgccaccc gcgcgcaccg tctcgcttgc gctcctgcac cacccgcgcc agcggaatcg
      121 agaacagcgc ccaccacgcc gccacggtca ggaacgacca gcgcgccgcg ctcgtggcat
      181 cgctcaaacc gaactgctcg ggcattcggt acatcaggat gttgaccgcg aagaggaggc
      241 cgccgcccag gtaccccagc gcgaagccgt acgacgagac gtagtccgac gtctcgggag
      301 tcgacacgtc gatgagcagg gagtcgtaga agatcatcga ggcggagaag ccgatggccg
      361 cgacgacgta cagagtcgcg gccgcggccc acgcgccgct gccgacgaag aacaggagcg
      421 tggtcgcggc gataccgatg gcggcactcg ccaagaggaa gcgcttgcgc gaaccgcccg
      481 cgtccgcgat cgcacccagc gccggtgcca ccagagcgat tcccaggctc gaaagcgaat
      541 tcgcgatccc gagccggtgc gtcgaaacgg ccgcgtccac gtccgcgcac cagtattgct
      601 tgaacaagag ggggaagaaa cccgccatca cggtggtgga gaaggcggag ttcgcccagt
      661 cgtagaaggc ccacgcggcc gccggccgat cgatgcgttt gcgcatgcgg gatcgtcgcg
      721 gaaaaacggc gttggttcgg tgcgcgacgt ttggatagac taggccccag cgcgccgcaa
      781 ggcaagcgaa aggagccccg caagctatgc caggcatcga catgaagcag ctcgacacgg
      841 tgttgaaaga ggcccgccgg ttcaagccgg cggagtcgtt ccgcaagggc gcccacctgc
      901 gctcggaggc cgaataccgc aagctttata aggagagcat ccagaacccg cgcaagttct
      961 gggcgcgcgt ggcgagcgaa ctccactggt tcaagaagtg gaacctcgtg ctcgacgaga
     1021 agaaggcgcc gttctaccgc tggttcgtgg gcgggaagac gaacctggcg tacaactgcc
     1081 tcgaccggca cctggattcg cccacgcgac acaaggccgc catcgtgtgg gagggcgagc
     1141 cgggggaccg ccgcgtgctg acgtacgcgg acctcgcgcg cgacgtgggc accttcgcca
     1201 acgcgctcaa gtcgctcggc atcaagaagg gcgaccgcgt cgccatctac ctcccgatga
     1261 cgcccgagct cgtggtatcg ctgctcgcgt gcgcgcgcat cggcgcggtc cactccgtaa
     1321 ttttcgccgg cttttccgcc gagtcgatcc gcgaccgcgt gcaggattgc caggccaagc
     1381 tcgtgatcac gggtgacggc gggtggcgcc gcggcaaggt tctccccctc aaggacatcg
     1441 tcgacgaagc gctggccgac gcgcccagcg tcgagaacgt catcgtcgtg cgtcgcaacc
     1501 ccgacagccc gttcccgtgt cacatgcgag acggccgcga ccagtggtac cacgagatca
     1561 ccgacgggat gtcggcggac tgccccgccg agaagatgga cagcgaggat atgctgttcc
     1621 tcttgtacac gagcggcacc accggcaaac cgaagggcat cattcacacc accggcggtt
     1681 acatggtgtt cacgtatctc accgcgaagt acgtcttcga tctcaagccc gaagatatgt
     1741 tctggtgcac cgccgacatc gggtgggtga ccggtcacag ctacatcgtc tacggcccgc
     1801 tgcagaacgg cgctacctgc ctcctgtacg aaggcgcgcc cgactggccc aaccgcggac
     1861 ggttctggga gatgatccag cgctaccggg tcaacgtgtt ctataccgcc cccaccgcga
     1921 ttcgcgcctt catgaagtgg ggcgacgaat ggcccgccaa gcacgacctc tcgtcgctgc
     1981 gcttgctcgg cagcgtgggc gagccgatca atccggaagc gtggatgtgg taccagaaag
     2041 cgatcggcgg gaagaagtgc ccgatcgtcg acacgtggtg gcagacggag acgggcggca
     2101 tcatgatcac gccgctgccc ggcgtcacgg ataccaagcc ggggagtgcc acgcgcccgt
     2161 tcttcggagt cgatgcggcc atcttgaacg acgccggcca ggaagtcgac gccggctatc
     2221 tcgcgattcg caagccctgg cccggcatgc tgcgcggcgt ctacggagat ccggagcgtt
     2281 acaagcagac ctactggtcg aagtggcccg gcgtctactt cccgggcgac ggcgcgcgca
     2341 aggacaagga cggctactac tggatcgtgg ggcgcgtcga cgacgtggtc aacgtggcgg
     2401 gacatcgcat cggcaccgcc gagttggaga gcatcttcgt cgagcacccg cgcgtggcgg
     2461 agtcggccgt gatcggcgtc gcgcacgaat tgaagggcca ggcgctggtc gccttcgtga
     2521 gcctgcgcga cggggtcatg ggcaccgagg cgctggacaa ggaactcaag gaatgggtcg
     2581 tgaagaagat cgggaagttc gcgatcccgg accgcatcgt gttcgcgggc gatctgccca
     2641 agacgcgctc gggcaagatc atgcggcgcc tcctgcgcga cgtcgccgag ggccgcgcgc
     2701 tcggcaacgt gacgacgctc gccgacgcgg gcgtggtgga gaagctaaag agccagtacg
     2761 aggaggacta gctgccgcgt ctctcgactc gagcaagaat gaaaacagcc ttccacgtcg
     2821 acgaaatcta ccttcgtcac cgtaatccag agggtcatcc cgaacgaggc gcgagactcg
     2881 aagcgttact cgagttggcg aagcatgccg atcgactcgg cgtcgacgtg atcccatcgc
     2941 gccggcgcgc gacggtcgaa gaactctccc gcgtgcacac tccagagtac gtcgagacga
     3001 ttgcatcgac cgcggggcga tcgctgatgc tcgaccccga cacgttcacc gtggccgact
     3061 cgtacgaggt cgcctgtcat gcggccgggt ccgcgctcga tctggtcgac cgcgtcatcg
     3121 cgcgcgaatt cagcaacgcc ttcgccgccg tgcgcccacc cggccaccac gccgagcgtg
     3181 agcgcgcgat ggggttctgc ctgttcaata acgtcgccgc ggccgcggcc cacgccgtgc
     3241 agcggcatgg aatggagcgc gtcctgattg tcgattggga cgtccaccac ggcaacggca
     3301 cccaagacat cttctggagc gatccacgtg tgctgttcgt atcgctgcac caattcccgt
     3361 tctatcccgg cagcgggcac gtcgaagaag tgggcgagga cgcggggcgc ggcttcacgg
     3421 tcaatatccc gatgccgggg gggttcggcg acgacgagtg gctggccgcg tttcgccgcg
     3481 tggtcgaacc ggtcgcgcac cagttcgccc ccgagttggt gctggtgtcg gccggcttcg
     3541 acgcgcacgc gaacgatccc ttgggcggta tgcgggtctc cgagcgcggg ttcgcggcga
     3601 tggccgattc gctgctaacg atcgcgcgcg agcacgcgga cggccgcttg atcgccgtgc
     3661 tcgaaggcgg ctacgacatc caggccctct ccacgagcgt cgaggcggta ctcgatcgtt
     3721 tgacgaactc cggtcccgcg gccccgtcgg ccgccgcgga cggccgattc ggcgaagtct
     3781 ttgcacgcat gcaggcggct caatctgcct actggaagct gtaaccgttc gctcccccga
     3841 gaggaacgac gatgcccctt acgaccttcg ccgccatcgc ggtttccgtc gcgcaatgcg
     3901 cggcggatgg caaagcgctg cccccggacc tcgcctcgct ggtcgaggcc gagcgcgcgt
     3961 tcgcgcaggc gtcggtgtcg aacggcgccc gggcggcctt tctcgagtac ctggct
//
LOCUS       DASPGK010000189         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1687841, whole genome shotgun sequence.
ACCESSION   DASPGK010000189 DASPGK010000000
VERSION     DASPGK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687841"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VEC56_05365"
     CDS             <1..238
                     /locus_tag="VEC56_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13616.1"
                     /translation="VQHAQATPVRPSSRTELAVPEALDRIILSCLAKNPDDRPASADA
                     LAEALAAIAIGEPWTTPRAHEWWERHKPAGAVSS"
     gene            256..660
                     /locus_tag="VEC56_05370"
     CDS             256..660
                     /locus_tag="VEC56_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13617.1"
                     /translation="MTFRIWAKSLVLWLVILVLAMLNGALREKVLIPALGSFAGLVGS
                     GVVLSICVFLVALCAAPWYGRLPRRQWWLIGMFWLLLTVGFEFGFGHFVQREPWSGLL
                     EAYTFQGGNIWPLVLVVTLVSPRLAARLRGVG"
     gene            677..1066
                     /locus_tag="VEC56_05375"
     CDS             677..1066
                     /locus_tag="VEC56_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXV13618.1"
                     /translation="MAVRIVRLGSPRAKGEGLRIGTVRRPPRGVPKDQFASRDFYDVW
                     FPNLAPTEPLLKASFPIQDERAWKNFRRRFLAEMKEPGARRDLELLAALSHHADFAIG
                     CYCETESHCHRSILRELLRELGAKIAG"
     gene            1196..>2089
                     /locus_tag="VEC56_05380"
     CDS             1196..>2089
                     /locus_tag="VEC56_05380"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HXV13619.1"
                     /translation="MELARIGGANTEPAYLFGQITFIAIADDNSVFVVDGQLNMIRHY
                     DPRGVYLAHVGRAGEGPGEYREIKGMLMLPSGELVVLSNPNRLTFFDGRSGAYLRDFT
                     VRSLLSSPRMLECDSAGFVYVKDVDGTPPMDAEWSKVWLKIDAAGKEAAVIPIPMENQ
                     APKAFHLYFPEGHRENFVVATKSALSRDGHVVSGRNDRYAFDIQGDPLVHIERDVEPV
                     ELVKEERSEWQAWARRFSRGGETYEIPKAKPAYREIYVDTDGLIWVHRYVAARKVKLD
                     PRPPGDDRPLSTWREPNTFDVF"
BASE COUNT          359 a          636 c          698 g          396 t
ORIGIN      
        1 ggtgcagcac gcgcaagcga cgcccgtgcg gccctcgtcg cgaaccgagc tagcggtgcc
       61 cgaagcgctc gaccggatta ttctctcatg cctcgcgaag aatcccgacg accggcccgc
      121 ttccgccgac gcgctggccg aggcactcgc agccatcgcg atcggcgaac cctggaccac
      181 gccgcgcgcg cacgagtggt gggagcggca caagccggcg ggtgcggtgt cgtcgtaacg
      241 aggcgagcgg tcgatatgac attccggatt tgggcgaagt ccctcgtgtt gtggctcgta
      301 attctcgtgc tcgccatgct caacggtgcg ctacgagaga aggtgctcat ccccgcgctc
      361 gggtccttcg cggggcttgt cggcagcggc gtcgttcttt caatctgcgt gttcttggtg
      421 gcgctgtgtg cagcgccgtg gtatggccgg ctccctcgac gtcagtggtg gctgatcggg
      481 atgttctggc ttctcctgac cgtcgggttc gaattcggct tcggtcattt cgtgcaacgc
      541 gagccgtggt cggggctcct ggaagcgtac acgttccagg gaggcaatat ctggccgctc
      601 gtcctcgtgg ttacgctcgt ctcgccccgg ctcgcggcgc ggctgcgcgg agttggttga
      661 aagcgagggt cgcatcatgg ccgtccgcat cgtaagactc gggagtccgc gcgcgaaggg
      721 cgaagggctg cgcatcggga ccgtgcgccg cccgccgcgc ggagtgccca aggaccaatt
      781 cgcgtcgcgc gacttctacg acgtgtggtt tcccaacctc gcgccgaccg agccgctcct
      841 caaagcgtcg tttcccattc aagacgagcg cgcgtggaag aacttccggc ggcggttcct
      901 cgcggagatg aaggagccgg gagcgcgccg cgacctcgaa ctcctcgcgg ccctctccca
      961 ccacgccgac ttcgcgatcg gctgctactg cgagaccgag tcgcactgtc atcgatcgat
     1021 tttgcgcgag ctcttgcggg agttgggggc gaagatcgcg gggtgaatca tgacaggagc
     1081 atcccgcata gtctggatca tcgtgcgtgc ctcgggagcg cgcgcgatgc tcatgatctc
     1141 gtttctcctc gtagggctca ccgccacgcc gcaatccgcg gggaccgtcg aacccgtgga
     1201 attggcgcgc atcggcggcg cgaacacgga gcccgcttac ttattcggtc aaatcacttt
     1261 catcgccatc gcagacgaca actcggtgtt tgtcgttgat gggcagctca acatgatcag
     1321 gcactacgat ccgcgaggtg tgtacctggc gcacgtgggc cgggcgggtg agggaccggg
     1381 cgagtatcgc gagatcaaag ggatgctgat gctgccttcc ggcgagctcg tggtgttgag
     1441 caatcccaac cggttgacct tcttcgatgg aaggagcgga gcatacctcc gggacttcac
     1501 tgtgcgaagt cttctctcgt cgccgcgcat gctcgagtgt gacagcgcgg ggttcgtcta
     1561 cgtgaaggac gttgacggca ccccgccgat ggatgcggaa tggtccaagg tgtggctcaa
     1621 gatcgacgcc gcgggcaagg aggccgccgt gatccctata ccgatggaga accaggcccc
     1681 gaaggcgttc cacctgtatt ttccggaagg ccatcgggag aatttcgtcg ttgcaactaa
     1741 gagtgcactg agcagggacg ggcacgttgt cagtggtcga aacgatcgct acgcgttcga
     1801 catccagggc gatcccctcg tgcacattga gcgggatgtc gaaccggtcg aactggtcaa
     1861 ggaggaacgc tccgagtggc aggcgtgggc gcggaggttt agtcgcggtg gcgagaccta
     1921 cgagattccg aaagcgaaac ccgcgtaccg cgagatctac gtggatacgg acgggctgat
     1981 atgggtccac cgatacgtcg cagcgcgaaa ggtaaagttg gacccacggc cccccggtga
     2041 cgaccggccg ttgtccacgt ggcgcgaacc caacacattc gacgtcttc
//
LOCUS       DASPGK010000190         5600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_165340, whole genome shotgun sequence.
ACCESSION   DASPGK010000190 DASPGK010000000
VERSION     DASPGK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165340"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..678)
                     /locus_tag="VEC56_05385"
     CDS             complement(22..678)
                     /locus_tag="VEC56_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV13620.1"
                     /translation="MKLQVMAAAGIAMVVASVASAEIRVTDRTQRWDLSIETRYTDSA
                     EFEGGYGTKLSLDDDLGWGFQFGYNLNERFNLGFLMSWRSISYNATYFSQTDPTSALN
                     YGGWLDTGTFAAMGQFNIFPNTFTPYVNGGIGWTLIDTNIVADYYTGCWYDPWWGYVC
                     NGYTSTYGTDTASFLVGVGVRLEPSEAVFIQAGYERAWLDDASSDGFNMVRVVLGFTN
                     "
     gene            complement(763..1530)
                     /locus_tag="VEC56_05390"
     CDS             complement(763..1530)
                     /locus_tag="VEC56_05390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXV13621.1"
                     /translation="MKTLPMVAIALSFAASVHAAIRVEPVEYKQGDTTLRGQLVYDDA
                     ATGKRPGVLVVHEWWGLNDYAKMRAKMLAEAGYVAFALDMFGDGKTTEHPEEAGKWAT
                     AIAANQQLGVDRFNAAWDLLKKNERVDAAHIAAIGYCFGGSTVLTMAFSGADLDGVAS
                     FHGITPAEPTGKVTASILVCIGAADPFIPAEQVATFQENVAAAGADWQLVLYADAKHS
                     FTNPGAGDRGLPALAYNEKADKRSWALLLDFLAEVCK"
     gene            complement(1572..2537)
                     /gene="corA"
                     /locus_tag="VEC56_05395"
     CDS             complement(1572..2537)
                     /gene="corA"
                     /locus_tag="VEC56_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HXV13622.1"
                     /translation="MLVNCVAYQKGQRVADITIGEIGSYLDRPDCFVWVAIKDPEPEE
                     LDALQKEFDLPALAVEDARHGHQRPKIEEYGSSLFVVLQPIEREGDELRTGEVAVFVA
                     DRYVLTVRRGAREGFVGVRRRTEQEPDLLKHGPAYVLYALMDTVVDRYFPIVDALTEE
                     IERIEERIFAGQTTRAHIEALYELKRDLMTIKHAAAPLLEVTGKLHGGRVPPMCMGLH
                     DYFRDVYDHLIRLNQSIDNLRDMATTATAVNLSLVQIQENEVTKRLAAWAALIAVPTM
                     VAGIYGMNFRHMPELSWKYGYPAVMAAIAAIDGYLVYRFRKAKWL"
     gene            complement(2613..4211)
                     /locus_tag="VEC56_05400"
     CDS             complement(2613..4211)
                     /locus_tag="VEC56_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13623.1"
                     /translation="MSRTFAFVLAMASAWVWAPSASARTCRITYLTSATAYVDAGLDE
                     GLAVGARIEVVRDGVVIATLVVTDASTHRAACAVESTTAALAVGDEARFAPRETKTSA
                     GSVAVPAQPQPTEAPAPTLQGRADPKLRALGLRGRVGVRFLGVFDQSGFGGDMTQPSA
                     DVRVDGTRVAGSSFDIQVDVRARHTTQSTAEGTDVNDGEARVYRLNTVWRSAKDRLRV
                     TAGRQFSSALTSVSTFDGVQAEYQRERWGGGVFAGTQPRPIDYGFSTDIQEYGAFLRT
                     RSAPRAPVRWEVVLAGIGSYEQGEINREYVAVLGRVASSRLSLILQQQIDVYRGWRQE
                     AEDKSSSITSTFTTARYRATRNLDLDLGYDNRRDVRVYHDYIDPEVAFDDSYREGVWG
                     GAALRFAQRFRVGAVVRSNGGGSAGDATSYTLTASASRVSPANLDLRVRSTRYDNERS
                     DGWMFAFSSGWAPDPRVTLGVTAGLREDNGKTIATPDVSTSWIGADLDLGLADGWYLN
                     LSGEHNGSGDDAYDQVYTGLSWRF"
     gene            complement(4208..5296)
                     /locus_tag="VEC56_05405"
     CDS             complement(4208..5296)
                     /locus_tag="VEC56_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13624.1"
                     /translation="MSTVRSYRVVFWVSIAVAGLLPIAVGRGRHSAFAADETVQTPNP
                     HGDYKEDCSLCHRPDGWKPARIGPKFDHEKFGVPLSGAHASVRCTQCHTNLEFSMAPT
                     ACVDCHADVHNGELGTDCARCHGTRNFIDRNDKIRMHRLTRFPLSGAHLTLDCEMCHQ
                     LASPDGLSWVNTPTDCESCHIEQFEATTNPDHVSAGFSQDCASCHNEQTWHRVRFDHA
                     GTSFPLTGAHVSVACDRCHIGGVYTGTPSACVACHQDDYDSTTDPSHIAAGFPTDCAA
                     CHTTSTWDGARFDHTQWFPIFTGVHRDKWSRCSDCHPNAASFAEFSCLGCHPHSDQAK
                     TDDQHQGENGYTYDSQACYTCHPQGRKP"
     gene            complement(5293..>5600)
                     /locus_tag="VEC56_05410"
     CDS             complement(5293..>5600)
                     /locus_tag="VEC56_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13625.1"
                     /translation="RIDARACRDCHPGPHGAQFDRRKDGGACESCHDAAHFVPASRFD
                     HARVKSFPLEGAHKKVPCGRCHPMVTPSDGKRMVLYRPTSSRCEDCHAGDGVLGGRS"
BASE COUNT          898 a         1837 c         1899 g          966 t
ORIGIN      
        1 atgccgccgt caatccgccg gctagttggt aaagccgaga acgacgcgaa ccatgttgaa
       61 accgtcggaa ctcgcgtcgt cgagccaagc ccgctcgtag ccggcttgga tgaacacggc
      121 ttcgctgggc tccagtcgca cgcccacgcc gaccaggaac gacgccgtgt cggtgccgta
      181 cgtcgaggtg tagccgttgc agacgtaacc ccaccagggg tcgtaccaac atccggtgta
      241 gtagtccgcg acgatgttgg tgtcgatcag cgtccacccg ataccgccgt tgacgtaggg
      301 tgtgaaggtg ttgggaaaaa tattgaactg acccatcgcg gcgaaggttc cggtgtcgag
      361 ccagccgcca tagttgagcg cagacgtcgg gtcggtctgg ctgaaatagg tcgcgttata
      421 gctgatcgac cgccaactca tgaggaatcc aaggttgaag cgctcgttca ggttgtagcc
      481 gaactggaac ccccacccga ggtcgtcatc caggctcaac tttgtcccgt agcccccctc
      541 gaactccgcg gaatccgtgt agcgcgtctc gatgctcaag tcccaacgct gggtgcggtc
      601 ggtgacgcgg atctccgcgg acgcgacgct cgcgaccacc atcgcgatgc cagccgcggc
      661 cattacttga agcttcatct tggccctcct cttcagacgt gattacggcg caaggtgcgc
      721 atcatatacc aggggtacac ccctgtctac ggccgagcag actcacttgc agacttcggc
      781 caggaagtcg agcagcagtg cccacgagcg cttgtccgcc ttttcgttgt acgcgagcgc
      841 cggcaagccg cggtctccgg cgcccgggtt ggtgaagctg tgcttggcgt ccgcgtacaa
      901 gaccaactgc cagtccgcgc ccgcggcggc gacgttctcc tggaaggtcg ccacctgctc
      961 cgcgggaatg aagggatcgg ccgccccgat gcacaccaga atgctcgcgg tgactttgcc
     1021 cgtgggctcg gcgggcgtga tgccgtgaaa gctcgcgaca ccgtcgaggt ccgcgccgga
     1081 aaacgccatc gtgagcaccg tcgatccgcc gaagcaatag ccgatggcgg caatgtgcgc
     1141 cgcgtcgacg cgctcgttct tcttcaagag atcccacgcg gcgttgaagc gatccacgcc
     1201 gagctgctgg ttggccgcga tcgcggtcgc ccacttgccc gcttcctccg ggtgctcggt
     1261 ggtcttgccg tcgccgaaca tgtcgagcgc gaaggccacg tagccggctt cggccagcat
     1321 cttcgcgcgc atcttcgcgt agtcgttcaa gccccaccat tcgtgcacca ccagcacgcc
     1381 ggggcgcttg cccgtggccg cgtcgtcgta gacgagctgg ccgcgcaggg tggtgtcgcc
     1441 ctgcttgtat tccaccggtt cgacgcgaat cgccgcgtgc acggatgccg cgaacgagag
     1501 tgcgatcgcc accattggca acgttttcat cggagtcctc cttgtcggcg ttgatcgaga
     1561 tacgagcggc gtcagagcca cttcgcttta cggaatcgat acacgagata gccgtcgatg
     1621 gcggcgatgg ccgccatcac cgcggggtag ccgtacttcc acgagagctc cggcatgtgg
     1681 cggaagttca tgccgtagat gcccgccacc atggtgggaa ccgcgatcaa cgcggcccac
     1741 gcggccagcc gcttggtgac ctcgttctcc tggatttgca ccagcgacag attgaccgcg
     1801 gtggccgtcg tggccatgtc gcgcaggttg tcgatggact ggttcaagcg gatcaggtgg
     1861 tcgtacacgt cgcgaaaata gtcgtgcagt cccatgcaca tgggcggcac gcgcccgccg
     1921 tggagcttgc ccgtcacttc cagcagggga gccgcggcgt gctttatcgt catgaggtcg
     1981 cgcttgagct cgtagagcgc ctcgatgtgc gcgcgcgtgg tctggcccgc gaagatgcgc
     2041 tcctcgatgc gttcgatctc ctccgtcagc gcatcgacga tgggaaagta gcggtcgacc
     2101 acggtgtcca tgagggcgta gaggacgtac gccggcccgt gcttcaggag atcgggctct
     2161 tgctcggtgc gccggcgcac gcccacgaaa ccctcgcgcg ctccccggcg cacggtgagc
     2221 acgtagcggt cggcgacgaa caccgcgact tcgccggtgc gcagctcgtc gccctctcgc
     2281 tcgatgggct gcagcacgac gaacagcgag ctcccgtact cttcgatctt ggggcgctgg
     2341 tgaccgtggc gcgcgtcttc gaccgcgaga gcgggcaggt cgaactcttt ctgcaaagcg
     2401 tccagttcct ccggctcggg atccttgatc gcgacccaca cgaagcagtc ggggcgatcg
     2461 aggtacgagc cgatctcgcc gatggtgatg tcggcgacgc gctggccctt ctgatatgcg
     2521 acgcaattga cgagcatgga gaacggcgcc gcggagcggg cgccgggtgg gccagtatat
     2581 caccgagcag ggcgggagga aaccgacggg cgttagaagc gccagctcag gccggtgtac
     2641 acctggtcgt aggcgtcgtc gcccgagccg ttgtgctcgc cggatagatt gaggtaccag
     2701 ccgtccgcga gtccgaggtc gaggtcggcg ccgatccacg acgtgctcac gtcgggggta
     2761 gcgatggtct tcccgttgtc ctcacgcagc cccgcggtga cgccgagggt cacgcgcggg
     2821 tcgggcgccc atccgctgga gaacgcgaac atccacccat ccgagcgctc gttgtcgtag
     2881 cgcgtcgagc gcacgcgcag gtcgaggttc gccgggctca cgcggctggc cgacgccgtc
     2941 agcgtgtacg aggtcgcatc gccggccgag ccgccgccgt tcgatcgcac caccgcgccc
     3001 acgcggaagc gttgcgcgaa tcgcagcgcg gcgccgcccc acacgccttc gcggtaggaa
     3061 tcgtcgaacg cgacttcggg gtcgatgtag tcgtggtaca cgcggacgtc gcggcgattg
     3121 tcgtaaccga ggtcgagatc gaggttgcgc gtggcgcggt agcgcgcggt ggtgaacgta
     3181 ctggtgatcg acgacgactt gtcttccgcc tcctggcgcc agccgcggta cacgtcgatc
     3241 tgctgctgca ggatcaacga caaccggctc gatgccactc ggccgaggac ggcgacgtac
     3301 tcgcggttga tttcgccctg ttcgtaggag ccaatgccgg ccagcacgac ttcccagcgc
     3361 acgggcgcgc ggggcgcgct gcgcgtgcgc aggaacgcgc cgtactcctg gatgtcggtc
     3421 gagaagccgt agtcgatagg cctcggctgc gtgcccgcga agacgccgcc gccccagcgc
     3481 tcgcgctgat actcggcctg gacgccgtcg aaggtgctca cactcgtcaa ggccgacgag
     3541 aactggcgcc cggcggtgac tcggagacgg tccttcgccg atcgccacac ggtgttcaag
     3601 cgatacacgc gcgcttcacc gtcgttgacg tcggttcctt ccgcggtcga ctgcgtggtg
     3661 tggcgcgcgc gcacgtcgac ctggatatcg aacgacgacc cggcgacgcg cgtcccgtcg
     3721 acgcgaacgt ccgccgacgg ttgtgtcatg tcgccgccga agccgctctg gtcgaacacg
     3781 cccaggaagc gaacgccgac gcgtccgcgc agccccaggg cccggagctt ggggtcggct
     3841 cttccctgca acgtcggtgc tggagcctcc gtgggctgcg gttgcgcggg gaccgcaacg
     3901 ctccccgcgc tggtcttggt ttcgcggggc gcgaagcgag cctcgtcccc gaccgcgagc
     3961 gcggccgtcg tcgactccac cgcgcacgcg gcgcgatgcg tcgaggcgtc ggtgacgacc
     4021 agtgtcgcga tcacgacacc gtcccggacc acttcgatcc tcgcgccgac cgcgagtcct
     4081 tcgtccaggc ccgcgtcgac gtacgcggtg gcactggtga ggtaggtgat acggcaggtc
     4141 cgcgccgagg cgctcggcgc ccacacccat gccgacgcca tcgccagaac gaacgcgaag
     4201 gtccggctca cggcttcctc ccttgcggat ggcacgtgta gcaggcctgc gagtcgtagg
     4261 tgtacccgtt ttcgccctgg tgctggtcgt cggtcttggc ctggtcgctg tgcgggtggc
     4321 aacccaggca cgagaattcg gcgaagctcg ccgcgttggg gtggcaatcg gaacagcgac
     4381 tccacttgtc gcggtgtacc ccggtgaaaa tcgggaacca ctgggtgtga tcgaagcgcg
     4441 cgccgtccca agtcgacgtg gtatggcacg cggcgcagtc ggtcgggaat ccggccgcga
     4501 tgtggctggg atcggtggtg gagtcgtagt cgtcctggtg gcacgcgacg cacgcgctcg
     4561 gcgtaccggt gtacacgccg ccgatgtggc accgatcgca cgccaccgac acgtgcgccc
     4621 cggtcagcgg gaagctggtg ccggcgtggt cgaagcggac ccggtgccag gtctgttcgt
     4681 tgtggcacga cgcgcagtcc tggctgaagc cggccgagac gtggtcggga ttggtggtcg
     4741 cttcgaactg ctcgatgtgg cacgactcgc aatcggtcgg ggtattgacc cagctcaacc
     4801 cgtccggcga ggccagctgg tggcacatct cgcagtcgag ggtgaggtgg gcgcccgaga
     4861 gcgggaagcg cgtcaggcgg tgcatccgaa tcttgtcgtt tcgatcgatg aagttgcgcg
     4921 tgccgtggca gcgcgcgcag tcggtgccca gctctccgtt gtggacgtcg gcgtggcagt
     4981 cgacgcatgc ggtgggcgcc atcgagaact ccaggttggt atggcactgc gtgcaccgca
     5041 ccgacgcgtg cgccccggaa aggggaacgc cgaacttctc gtggtcgaac ttggggccga
     5101 tgcgcgcggg cttccagccg tccgggcggt ggcacaggct gcagtcttcc ttatagtcgc
     5161 cgtgcgggtt gggcgtctgc accgtctcgt cggcggcgaa cgcgctgtga cgcccgcggc
     5221 ccaccgcgat cggcaggagt ccagcgaccg cgatactcac ccaaaaaacg actcggtacg
     5281 atctaacggt actcacgatc gacctcccaa gacgccgtcg ccggcgtggc aatcctcgca
     5341 gcgcgacgac gtcggacggt acaacaccat ccgcttgcca tccgacggcg tgaccatggg
     5401 gtggcagcgg ccgcacggga ccttcttgtg cgcgccttcc agcgggaacg atttcacgcg
     5461 cgcgtgatcg aagcggctcg cgggcacgaa gtgcgccgcg tcgtgacagc tctcgcacgc
     5521 gccaccgtcc ttgcgacgat cgaactgcgc gccgtgaggg ccggggtggc aatcccggca
     5581 cgcgcgggcg tcgatccgga
//
LOCUS       DASPGK010000191         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1335702, whole genome shotgun sequence.
ACCESSION   DASPGK010000191 DASPGK010000000
VERSION     DASPGK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335702"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="ndhC"
                     /locus_tag="VEC56_05415"
     CDS             complement(<1..277)
                     /gene="ndhC"
                     /locus_tag="VEC56_05415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXV13626.1"
                     /translation="MELALKYLPLIVLFLLAVMIPIIMLAIAGVTGPRQPSPRKMTPF
                     ESGIESLGDTRHRFSVRFFLVALLFIIFDIEAVFIFPWAVLFRELGLF"
     gene            540..1184
                     /locus_tag="VEC56_05420"
     CDS             540..1184
                     /locus_tag="VEC56_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXV13627.1"
                     /translation="MRSPVAGEVKTRLEPALTADEARDVYVAFMADFFARLRDSKYRP
                     TVFLSGGRSAGLEAILDPKWSVVAQGDGDLGARLTAAFAVLLRNPGDRAVIVGSDSPD
                     LPLAYLKKAFQSLKHRDVVIGPAIDGGYYLIGLRAPAPALFRDVHWGAANVLDETLDR
                     VDDERLSLALLPPWYDVDDAASLRFLADLNRARRIARQEPLRHCERALAALFKG"
     gene            1188..1919
                     /locus_tag="VEC56_05425"
     CDS             1188..1919
                     /locus_tag="VEC56_05425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV13628.1"
                     /translation="MFPLKDHNPTRTRPYVTMLFVAINVVVFAYEMMLGERLDGFVAA
                     WGAIPYEITRFEDLVGPDQYFEHQAGPAFLPLTLISSMFMHGSVMHIFGNMLYLWIFG
                     NNVEDVLGHVKFVFFYIVCGVLAGLAHIFFNPNSTVPTVGASGAVAGVLGAYMVMYPK
                     ARVLTLVFLGIFIRMMEVPAAVLLVLWFVMQSLQGFLSLGVREMSGGVAWFAHIGGFV
                     AGAVLSLAMAGGTIREIRGRSRYDD"
     gene            complement(1927..2574)
                     /locus_tag="VEC56_05430"
     CDS             complement(1927..2574)
                     /locus_tag="VEC56_05430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV13629.1"
                     /translation="MQVLYLIRHAETGFNRHGRVQGHTESTLSRLGIEQAKRIGKRLD
                     YVDFVAAYASPSKRTMQTARLAFDDTVEIHAREGLREIRLGKWEGEKATTLRRRYPRQ
                     VKLWFHQPSAVRIPGAETVAQFRRRVVRAMNGIREAHPSGEIAIVTHGGVICAYLTAA
                     LGMKLDDLWSFKIRNGSVTRVLFPQGRARIDLLGDIHHLNGAFRESPKRPFRLFP"
     gene            2632..3267
                     /locus_tag="VEC56_05435"
     CDS             2632..3267
                     /locus_tag="VEC56_05435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV13630.1"
                     /translation="MISFIPETIEAYADAHSTPEPALFAELAAVTRAQTTKPQMMVGH
                     TQGLLLKFLVRIAGAKRVLEIGTFTGYSALAMAEGLPDDGSIVTCDIDPKATAIARSF
                     WERSPHGRKITLALAPALDTIATLAGPIDFVFIDADKTNYVRYWDACVPKVRPGGVLA
                     ADNVLWSGEVLDPKTDDARALAAFNEHVLRDARVDLVMLPIRDGVTLAVKR"
     gene            3289..3777
                     /locus_tag="VEC56_05440"
     CDS             3289..3777
                     /locus_tag="VEC56_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13631.1"
                     /translation="MIVTAIKIVLLIAFVILIALAIGLVVMAGVAQSHDVVRIPVPQS
                     TFVAGTEPKSIYSDAYVAPMEYSTFRDIDRVAQLAFHRGTKEVHRDEREVAYEGTMLG
                     LSYTISYILMKDSSPQTLTVATTVRIVEPKKGKYCWMVAKHVHRHLLPYLLDRMVITA
                     PD"
     gene            3896..5719
                     /locus_tag="VEC56_05445"
     CDS             3896..5719
                     /locus_tag="VEC56_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXV13632.1"
                     /translation="MKKRLSLLAGVSAALIAAVPGHAQFTDPFAGEPVSATGYVSLSN
                     VPQGGSAQLALEIKITAPWHVNANPASEEFLVPTEVAFELPPGVTLRGIVYPPGAEMK
                     LAFAEAPLRLYDGTVYVGAVIDVASDVPIDTSIVTATITYQACDNEKCLLPQTLEVPI
                     LLAISSPRDPVDLASPEVFDRIDFTAVLGKTGDGAGSDGNALKDAIAKRGWLFAFVLV
                     FLGGLALNLTPCVYPMIPITVSYFGGQAKGRGGRTVLLAALYLLGMAVMYSTLGLVAA
                     LTGSLFGSALQNPIVLAVIALVMVGLALSMFGVYEIRVPTRLAGVAGTAKQGTVGSFL
                     MGLTVGIVAAPCIGPFVLGLLTFVGEVGNPLLGFSLFFVLALGLGLPFVVLAIASGNI
                     TRLPKSGEWMEWVRKLFGIVLLAMAAYFLRPIVGDTLYFILLGAVLVVGGVMLGFVSK
                     VKTSSLFFSGFRRFVGVVAPLYGLYLVLAPGNILGRSAVAGIPWIPYEEAALVQAAEQ
                     GKAVVIDFAAEWCLPCKELDHLTFNQPEVIEAAKGIVPLKADLTQHGSPAVRELRKKF
                     DIRGVPTIVFIDATGRERQNLRAVEFIDKEEFLSRLSKLTS"
BASE COUNT          940 a         1866 c         1882 g         1091 t
ORIGIN      
        1 cgaacagtcc cagctcgcga aacaggacgg cccacgggaa tatgaagacg gcttcgatgt
       61 cgaagatgat gaagaggagc gcgaccagaa agaagcgcac cgagaagcga tggcgcgtat
      121 cccccagcga ctcgatgccg ctctcgaagg gcgtcatctt gcgcgggctg ggctggcgcg
      181 ggcccgtgac accggcgatg gccagcatga tgatgggaat catcacggcc aacagaaaca
      241 ggacgatgag gggcaagtat ttgagcgcga gctccatgat aatgcggcct gcgacacctg
      301 tcggtttggg ggtgatcggg aagccttgtc gtgggcttgt aaccttattc acaaagactt
      361 ccgcgcatga aggtactgcg aacgcgcggg agtgtcaacc gcctgttcgc gacacccgag
      421 tcgctccgga ttcgccgcgt cgacgcccga ccgcgcgtgg tagagtgtcg ggcatggccg
      481 cgcgcccaca agccccggaa accagcaata ccctaccgag aaacaggtta ggcgtcttcg
      541 tgcgctcacc ggtggcagga gaggtaaaaa cgcggctcga gccggcgctc acggcggacg
      601 aggcgcgcga cgtgtacgtc gccttcatgg ccgacttctt cgcccggctg cgcgattcca
      661 agtaccgccc gacggtgttt ctctcgggag gtcgatcggc cggactggag gcgatactcg
      721 acccgaagtg gtccgtcgtg gcgcaaggcg acggcgattt gggcgcccga ttgacggccg
      781 cgttcgcggt gttgcttcga aaccccggcg atcgcgccgt gatcgtgggc tcggacagtc
      841 ccgacctccc gctcgcttac ctcaagaagg cattccaatc gctcaagcac cgcgacgtcg
      901 tgatcgggcc tgccatcgac ggcggttatt acctgatcgg gctgcgcgcg cccgcacccg
      961 cgttgtttcg cgacgttcac tggggagctg cgaacgtact cgacgagacg ctggatcgcg
     1021 tggacgacga gcgattgtcg ctggcactcc tgccgccgtg gtacgacgtc gacgacgcgg
     1081 cgtcgcttcg attcctcgcg gatctgaacc gcgcgcgtcg catcgcgcga caggagccgc
     1141 ttcgccattg cgagcgcgct ctggcggccc ttttcaaggg gtaacccgtg tttccgctca
     1201 aggaccacaa ccccacgcgc acgcgccctt acgtgacgat gctcttcgtc gcgatcaacg
     1261 tcgtcgtgtt cgcctacgag atgatgctcg gggaacggtt ggacggcttc gtggctgcgt
     1321 ggggtgccat tccctacgag atcacgcgct tcgaggattt ggtgggaccc gatcagtact
     1381 tcgagcatca agccggaccg gcgttccttc cgctcaccct gatttcgtcg atgttcatgc
     1441 acgggagtgt catgcacatc ttcggcaaca tgctttacct gtggattttc gggaacaacg
     1501 tggaagacgt gctggggcac gtgaagttcg ttttcttcta cattgtgtgc ggtgtgctgg
     1561 ccggactcgc acacatcttc ttcaatccca actcgaccgt cccgacggtg ggtgccagcg
     1621 gtgccgtggc cggtgtactg ggcgcgtaca tggtgatgta tcccaaggcg cgcgtgctga
     1681 ccctcgtgtt cctcggcatc ttcattcgca tgatggaagt cccggccgcc gtgctgctcg
     1741 tgctctggtt cgtgatgcag tcgctgcagg gattcctgtc gctgggcgtg cgcgagatgt
     1801 cgggcggggt ggcctggttc gcccacatcg gggggttcgt ggcgggcgcg gtgctgtcgc
     1861 tcgcaatggc gggcgggacg attcgggaga ttcgcgggcg gtcgcgctac gacgattgaa
     1921 cgccgcctac ggaaacagcc gaaacggccg cttgggcgat tcgcggaacg cgccgttgag
     1981 atgatggatg tcgcccagca gatcgatgcg cgcccggccc tgcggaaaga gcacccgcgt
     2041 caccgagccg ttgcggatct tgaagctcca tagatcgtcg agcttcattc cgagcgccgc
     2101 ggtcaggtac gcgcagatca caccgccgtg ggtcacgatc gcgatctccc ccgacgggtg
     2161 cgcctcgcga atgccgttca tggcgcgcac cacacggcga cgaaactgcg ccacggtctc
     2221 ggctccgggg atgcgaacgg cgctcggctg gtggaaccag agcttcacct ggcgcgggta
     2281 gcggcgccgt agcgtggtcg ctttctcgcc ctcccacttc cccaagcgaa tctcgcgcaa
     2341 cccctcgcgc gcgtgtatct ccacggtgtc gtcgaaggcg aggcgcgcgg tctgcatggt
     2401 gcgcttcgac gggctcgcgt acgcggcgac gaagtcgacg tagtcgagac gtttcccgat
     2461 gcgcttggcc tgctcgatcc cgagccggct caaggtcgac tcggtatggc cctggacgcg
     2521 cccgtggcgg ttgaagccgg tctcggcgtg ccggatgaga tagagaacct gcatggagac
     2581 accaagatag gggcttggtc agggactgtc cagcggcgta tgctccccgc gatgatcagc
     2641 ttcatccccg agaccatcga ggcgtatgcg gatgcgcatt ccacacccga gcccgcgctc
     2701 ttcgccgagc tcgccgcggt gacgcgcgcg cagacgacga agccccagat gatggtcggt
     2761 cacacccagg gcttgctgct caaattcctc gtgcgcattg cgggtgcgaa gcgcgtgctc
     2821 gagatcggga ccttcaccgg ctacagcgcg ctcgccatgg ccgaaggact ccccgacgac
     2881 gggtcgatcg tgacctgcga catcgatccc aaggcgaccg cgatcgcgcg ctcgttctgg
     2941 gagcgaagcc cgcacggccg caagatcacg ctcgcgctcg cgccggccct cgacaccatc
     3001 gcaaccctcg cgggcccgat cgactttgtc ttcatcgacg ccgacaagac caactacgtt
     3061 cgttactggg acgcgtgcgt ccccaaggtc cgcccgggcg gcgtgctcgc ggccgacaac
     3121 gtgctgtgga gcggcgaggt gctggacccc aagaccgacg atgcgcgcgc gctggcggcg
     3181 ttcaacgagc acgtgctgcg cgatgcacgc gtcgatttgg tcatgctgcc gattcgcgac
     3241 ggcgtgaccc tcgcggtgaa gcggtaacat ggggagccgg aggaacctat gatcgtcacc
     3301 gccatcaaga tcgttctctt gatcgccttc gtgatcctga tcgcgctggc gatcggactg
     3361 gtcgtcatgg cgggcgtagc gcagagccac gacgtcgtgc gcatcccggt gccgcaatcg
     3421 accttcgtcg ccggcaccga gcccaagtcc atctactccg acgcgtacgt cgcgcccatg
     3481 gagtactcga ccttccgcga catcgaccgc gtcgcccaac tggcgtttca tcgcggcacc
     3541 aaggaagtcc accgcgacga gcgcgaggtg gcgtacgagg gcaccatgct cgggctgagc
     3601 tataccatct cgtacatcct catgaaggac tcctcgccgc agaccctgac ggtggcgacg
     3661 acggtccgca tcgtcgagcc gaagaagggc aagtactgct ggatggtggc caagcacgtc
     3721 catcggcatc ttttgcccta tctgctcgat cgcatggtca ttacggcgcc ggactgatga
     3781 cctccggccg gccgttgccg tggacgcccg gacgcgctat catcccgcaa ccaacccagc
     3841 gggcgcggtg taccacgtgg tgaccctccg cgcaggagtt cttccaaaac ccattatgaa
     3901 aaaacgactt tcgctgcttg caggggtgtc tgccgcgctc atcgcggcgg tgcccggcca
     3961 cgcccagttc accgatccgt tcgccggcga gccggtttcg gccaccggct atgtgtcgct
     4021 ctcgaacgtg ccccaaggcg ggtcggcgca actggcgctg gagatcaaga tcaccgcgcc
     4081 ctggcacgtc aacgcgaacc cggcctccga ggagtttctc gttcccaccg aggtcgcctt
     4141 cgagctgccc ccgggtgtga cgctgcgcgg catcgtctac ccgccagggg ccgaaatgaa
     4201 gctcgccttc gccgaggcgc cgcttcgctt gtacgacggc accgtgtacg tcggtgcggt
     4261 gatcgacgtc gcgagcgacg tgccgataga cacctcgatc gtcaccgcca ccattaccta
     4321 ccaagcgtgc gacaacgaga agtgcctgct cccgcaaacg ctcgaggtcc caatcctact
     4381 cgcgatctcg tcgccacgcg atccggtcga cctcgcgagt ccggaggtct tcgaccgcat
     4441 cgacttcacc gccgtgctcg gtaagaccgg cgacggcgcg ggcagcgacg gcaacgcgct
     4501 caaggacgcc atcgcgaagc gcgggtggct gttcgcgttc gtactcgtgt tcctcggcgg
     4561 tctggcgctc aatctgacgc cgtgcgtgta tccgatgatt ccaattacgg tgagttattt
     4621 cggcggccag gccaagggac gcggcggtcg taccgtgctc ttggccgcgt tgtatctgct
     4681 cggcatggcg gtgatgtact ccacgctggg gctggttgcc gcgctgacgg gaagtctctt
     4741 cggctcggcg ttgcagaatc ccatcgtgct ggccgtgatc gcgctcgtga tggtgggcct
     4801 ggccctctcg atgttcggcg tctacgagat ccgtgtgccg acgcgcctcg ccggtgtcgc
     4861 gggcacggcc aagcagggca ccgtcggttc ctttctgatg ggactgacgg tgggcatcgt
     4921 cgccgcaccc tgcatcggac cgttcgtgct ggggctgttg accttcgtcg gcgaagtcgg
     4981 caaccctctg ctcggctttt cgctcttctt cgtcctggcg ctgggcctcg ggcttccctt
     5041 cgtggtgctc gcgatcgcgt cgggcaatat tacgcggctt cccaaatcgg gcgagtggat
     5101 ggaatgggtg cgcaagcttt tcggcattgt gttgctcgcg atggcggcgt acttcctgcg
     5161 tccgatcgtc ggcgacacgc tgtatttcat cctgctgggc gccgtgctgg tggtcggtgg
     5221 cgtcatgctg ggtttcgttt ccaaagtgaa gacgtcctcg ctgttcttca gcggcttccg
     5281 gcgcttcgtg ggcgtggtgg ccccgctgta cggcctctac ctcgtcttgg cgccgggtaa
     5341 catcctcggg cgcagcgccg tcgcgggcat cccctggatc ccgtacgaag aagccgcgct
     5401 cgtgcaggcg gccgagcaag gcaaggcagt cgtcatcgat ttcgcggccg agtggtgctt
     5461 gccgtgcaag gagctcgacc acctcacgtt caaccagccc gaggtgatcg aggcggccaa
     5521 gggcatcgtg ccgctcaagg ccgacctgac gcagcacggt tcccccgcgg tgcgcgagct
     5581 gcgcaagaag ttcgacatcc gcggagtccc gaccatcgtg ttcatcgacg cgaccgggcg
     5641 cgagcgccag aacctgcgcg cggtcgagtt catcgataag gaagagttcc tgtcacgtct
     5701 gagcaaactg acgagttagc gacatgcgat tggtgagttt cggccccgcc ggtgaggaac
     5761 gacccggcgt cgtcgcggg
//
LOCUS       DASPGK010000192         3607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_634603, whole genome shotgun sequence.
ACCESSION   DASPGK010000192 DASPGK010000000
VERSION     DASPGK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634603"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /gene="purH"
                     /locus_tag="VEC56_05450"
     CDS             <1..1484
                     /gene="purH"
                     /locus_tag="VEC56_05450"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXV13633.1"
                     /translation="GDAGLSLIASSGTKTFLEEKGLAVREISDYTKSPEILGGRVKTL
                     HPKIHGGILADRGNPEHVRTLDEQATDPIDFVIVNLYPFEQKFQSGMSPDELIEYIDI
                     GGITLIRAAAKNHKHVTVLTHPSQYADAIAELKSSGATSLATRARLAAHAFALTATYD
                     AAISHAFRGALPAEQLPERLAIGLSKVTDVRYGENPHQRGALYRTVVDSPVVAMQQLQ
                     GKELSFNNYLDIMGAFGLVRDLGPGSVAIIKHNNPCGAAWLSDVLASWRRALKTDPVS
                     AFGGVVAVNGTVTPALAEEMGQLFLEVVIARDVDAGARGVLEKKKNVRVVTIPAAHWE
                     AGKPGLMGVWMQDAALVQDDDAGFPEWDRAETVTRRKPRAEEMTALRMAWIVAKHVKS
                     NAIVVTDAEGTVGIGAGQMSRVDSSHLAAHKAAKAGLAVKGAVAASDAFFPFADGVQE
                     LAKVGIAAVVQPGGSIRDAEVIAAADDANLAMLFTGRRHFRHV"
     gene            1487..3025
                     /gene="guaA"
                     /locus_tag="VEC56_05455"
     CDS             1487..3025
                     /gene="guaA"
                     /locus_tag="VEC56_05455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXV13634.1"
                     /translation="MPARGIVILDFGSQFTQLIARRIRSKGVFSEVLPWNAARERVLE
                     HDPAGVILSGGPASVFEAGAPTIPKDIWSWDRPVLGICYGMQLVARDLGLSVERAGKA
                     EYGHTRIRANPDHPLFRNTPVEQSVWMSHGDTVRGADGAVAVLATSAEGEMAAFHVPP
                     KNAYAVQFHPEVHHTAFGDTILENFLFSVCKAERTWSTGAIVAQMVDEIRAAVGDGRV
                     VCAVSGGVDSSVVACLIDKAIGKKLHCVFVDNGLLRAGEREGVVDLLGSHLSAPLDVV
                     DARARFLERLKGVDDPEEKRKRIGNLFIEIFEEVSTKRGPFEFLAQGTLYPDRIESFS
                     TGGPSVTIKTHHNVGGLPKNMRFKLVEPLALLFKDEVRAVGAELGLPRDQLSRHPFPG
                     PGLAVRVLGEVTEEKLAILRQADAIFVDALREFGLYEKVWQALAVLLPVKSVGVMGDA
                     RTYAFPIVLRAVTSTDGMTADWAELPTEFLREVSNRIINRVPHVNRVVYDVSSKPPST
                     IEWE"
     gene            3235..>3607
                     /locus_tag="VEC56_05460"
     CDS             3235..>3607
                     /locus_tag="VEC56_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13635.1"
                     /translation="MAPSRRAALLASHGEPRLAWDALRSGGPARTDADAVLAVRLLTE
                     LGRYAQAESLLAREAAPKSDRAATRYYLQRARLSFDAGRAERALELLDAAPAPAGDPL
                     SAYHEFVRAQSLAKKGDGAAAA"
BASE COUNT          587 a         1228 c         1224 g          568 t
ORIGIN      
        1 ttggcgacgc gggactctcg ctcatcgcct cgtcggggac caagacgttt ctggaagaga
       61 aggggctcgc ggtgcgcgag atctccgact acacgaagag ccccgagatc ctgggcgggc
      121 gcgtgaagac gctgcacccc aagatccacg gcggcatcct cgcggaccgc ggcaaccccg
      181 agcacgtgcg caccctcgac gagcaggcca ccgacccgat cgacttcgtg atcgttaatc
      241 tgtatccctt cgaacaaaag ttccaatccg ggatgtcgcc cgacgagctg atcgagtaca
      301 tcgacatcgg cggcatcacg ctgattcgcg ccgcggccaa gaaccacaag cacgtcaccg
      361 tgctgacgca cccgtcgcag tatgcggacg cgatagccga gctgaagtcg agcggagcga
      421 cgtcgcttgc cacgcgggcg cgcctggcgg cgcacgcgtt cgcgctgacc gcaacctacg
      481 acgccgccat ctcgcacgcc ttccgcggcg cgcttccggc cgagcagctc ccggagcgcc
      541 tcgcgattgg gctctccaag gtaacggacg tgcgctacgg cgagaacccg caccagcgcg
      601 gcgccctcta tcgaacggtg gtggattcgc cggtggtcgc gatgcagcag ctccagggca
      661 aggagctctc gttcaacaat tatctcgaca tcatgggcgc gttcggactc gtgcgcgacc
      721 tcgggcccgg cagcgtcgcc atcatcaagc acaacaaccc gtgcggggcg gcgtggctct
      781 cggacgtgct cgcgtcgtgg cgccgcgcct tgaagaccga ccccgtgagc gccttcggag
      841 gtgtggtggc cgtgaacgga accgtgacac cggcgctggc cgaagagatg gggcagttgt
      901 tcctcgaagt cgtcatcgca cgcgacgtcg atgccggtgc gcgcggcgtg ctcgaaaaga
      961 agaagaacgt gcgggtggtg acgatcccgg ccgcgcattg ggaagcgggt aagccggggc
     1021 tcatgggcgt gtggatgcag gacgccgcac tcgtgcaaga cgacgacgcc ggcttcccgg
     1081 aatgggatcg cgcggagacg gtgacgcgcc gcaagccgcg cgcggaggag atgaccgcgc
     1141 tgcgcatggc ctggatcgtg gccaagcacg tcaagtcgaa cgccatcgtc gttaccgacg
     1201 cggaggggac ggtgggcatc ggcgcggggc aaatgagccg cgtcgactcg tcgcacttgg
     1261 ccgcgcacaa ggcggccaag gcggggctcg cggtcaaggg cgcggtcgcg gcctcggacg
     1321 ccttctttcc cttcgccgac ggcgtgcagg agctcgcgaa ggtcggcatc gccgcggtgg
     1381 tgcagccggg cggatcgatt cgcgacgccg aggtgatcgc ggccgccgac gacgccaacc
     1441 tggccatgct cttcaccggg aggcgacact tccgtcacgt gtgaccatgc ccgcgcgcgg
     1501 aatcgtcata ctcgacttcg gctcccagtt cacgcagctc atcgcgcgcc gcattcgctc
     1561 gaagggcgtg ttctcggagg tgctgccgtg gaacgcggcg cgcgagcgcg tcctcgagca
     1621 cgatcccgcg ggcgtcattc tctccggcgg gccggccagc gtgttcgaag ccggggcacc
     1681 cacgatcccc aaagacatct ggtcgtggga ccgaccggtg ctcgggatct gctacggcat
     1741 gcagttggtc gcgcgcgatc tcggactatc cgtcgaacgc gccggcaagg ccgaatacgg
     1801 acacacccgc atccgcgcca accccgatca tcccttgttt cgcaacacgc ccgtcgagca
     1861 atcggtgtgg atgagccacg gcgacaccgt gcgcggcgcc gacggcgcgg tggcggtgct
     1921 ggccacgtcg gccgaaggcg agatggcggc cttccacgtg ccgcccaaga acgcatacgc
     1981 ggtgcaattc caccccgagg ttcaccatac cgccttcggc gacaccatcc tcgagaactt
     2041 tctcttttcg gtgtgcaagg ccgagcgcac ctggtcgacg ggcgcgatcg tcgcacaaat
     2101 ggtggacgag attcgcgccg ccgtcggcga cgggcgcgtc gtgtgcgcgg tcagcggcgg
     2161 cgtggacagc tcggtggtcg cctgcctgat cgacaaggcg atcgggaaga agctccactg
     2221 cgtctttgtc gacaacggac tgttgcgcgc gggtgagcgc gaaggcgtgg tcgacctgtt
     2281 gggctcgcac ctctccgcgc cgctcgacgt ggtcgatgcg cgcgcgcgtt tcctcgagcg
     2341 cttgaagggt gtggacgacc ccgaagagaa gcgcaagcgc atcggcaacc tattcatcga
     2401 gatcttcgag gaagtgagca cgaagcgcgg gcccttcgaa ttcctcgccc aagggacact
     2461 ctacccggac cgcatcgaga gtttttcgac cggcgggccg tcggtgacga tcaagaccca
     2521 ccataatgtg ggcgggctcc ccaagaacat gcgcttcaag ctggtcgagc cgctggcact
     2581 gctcttcaag gatgaggtgc gggcggtcgg cgccgaactc ggcctgccgc gcgaccagct
     2641 cagccgccac ccgttcccgg ggcccggcct cgcggtgcgc gtgctgggcg aagtcaccga
     2701 agaaaagctc gcgattctgc gccaagcgga cgctatcttc gtcgacgcgc tgcgcgagtt
     2761 cggcttgtac gagaaggtct ggcaggcgct cgccgtgctc ctgccggtca agagtgtggg
     2821 cgtcatgggc gacgcgcgca cgtacgcgtt tccgatcgtg ctgcgcgccg tgaccagcac
     2881 cgacggaatg acggccgact gggccgagct tcccaccgag ttcttgcgcg aggtctccaa
     2941 ccgcatcatc aaccgcgtgc cgcacgtcaa tcgggtcgtc tacgacgtga gctcgaagcc
     3001 ccccagcacc atcgagtggg agtagcgggc gcgtcgcggc atgtgaagtc gcgcacactc
     3061 cttgcttgtt tctggtcatg atccactctc actgcctcgc gccggttgcc ggagttctcc
     3121 ggcgcgcctt ataattgatc catgcaaccc atgctgcgtc gttgggtcat tttgaccgga
     3181 gccgtcgcgt gcgtttcggc aggccctgca cgatcggatt cgaccgacgg cgggttggct
     3241 ccgtcgcgcc gcgccgcgct cctcgccagc cacggcgagc cccggttggc ctgggacgcg
     3301 ctgcgctccg gcggacccgc gcgcacggac gccgacgcgg tcctggccgt gcgcctgctc
     3361 accgagctcg gtcgctacgc gcaagcggag agcctgctgg cgcgcgaggc cgcgcccaag
     3421 agcgaccgcg ccgcgacccg ctactacttg cagcgagcgc gcttgagctt cgatgccggt
     3481 cgcgccgagc gcgcgctgga gctcctcgac gccgcccccg cgcccgccgg cgacccgctc
     3541 tccgcgtacc acgagttcgt gcgcgcgcaa tcgctggcga agaaaggcga cggcgcggcg
     3601 gccgcgg
//
LOCUS       DASPGK010000193         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_752370, whole genome shotgun sequence.
ACCESSION   DASPGK010000193 DASPGK010000000
VERSION     DASPGK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752370"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VEC56_05465"
     CDS             <1..1058
                     /locus_tag="VEC56_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13636.1"
                     /translation="LNEIDAFHPPVVDSAQTWAEWHYFNYHDDAGRSLYVSISGIATE
                     GDLAPSGYSRAVVLFEMVDHTGARTKIVDVLPGREIAASHRSADLVAGLNTVHLEAGV
                     YRVHLALADSLARPITVVLELTPTPHQVIPPITPMGSEVPFGYVVPVVRGHMRGRVTI
                     GGTTFPLDGVGYHDHNWGHFRDAVWDWGIVHAGEVSVLYGRFATSMDALANQPVLFAL
                     FDAAGPRPFVLTHDYTVLWRPVEARRPSGPPRTRITQNTTASDTPSSEPASVLLSAVS
                     GDDSLSIRIDVDRRFVAETGGGEQPLFRADETRRSFFFQLEGRARLTGRVGGERFNAE
                     GHAYSETFRVDATAPRR"
     gene            complement(1039..3174)
                     /locus_tag="VEC56_05470"
     CDS             complement(1039..3174)
                     /locus_tag="VEC56_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13637.1"
                     /translation="MGKMRATLAVVCGVLCVAGLVFGVVFTYTSRTLFDASRFSQRAA
                     DSLAEPAVARVVAAEVTDQIIEARRDLTAYRPIILGTVEYVVSSAPFRALIRRAAKRA
                     HATLISETGEDLALAAADLGVVVRNALAMYPEIAEKIPERAQLVLGSPEDWPNGKLLQ
                     RALRIGHRLRVRSVVWLSVGFAAGVCGLWLTRRRDRYLLRVGIGLTITSLVLGAVARF
                     GGEVMAAFAQSPVVADLVRGMWPVFVGPLALRMLILAAIGLVLVAGVTSLLQKVDLES
                     LARTLWRRIETRPLRSRWAFPRALLFICAGMLVVLFPSHAIDVIFVIAGGALFFVGIQ
                     EVFTIAARFFPQAQAAIEAATERKRSAWPRVAVVGGLVLALAGAGAYWLSRDDDGVVA
                     AAGSALDPCNLHPELCDKRLNDVAFATTHNSMAAADISDWMFPNQERGLRAQLEEGVR
                     GFLIDIHFGVPVGDRIRTELEDEKASMEKYTEALGAEGVDAAMRIRDRLVGEPSGDRE
                     VYLCHGFCELGAERFFDALEGVRGFLVANPREVLIIVIQDEGVAPADVATCFEKSGLV
                     EFVYQGSVTPPWPTLREMIDGDQRVLVFAENNAEGVPWYHHVWEVFQETPYRFLDPAE
                     FSNKPGRGGTSGSLLLMNHWIETTPAPKPSNAEVVNAYDFLLKRARACKRERRMVPNL
                     IAVDFYGTGDLLRVVDAMNGIEEPTVVAP"
     gene            3276..3863
                     /locus_tag="VEC56_05475"
     CDS             3276..3863
                     /locus_tag="VEC56_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13638.1"
                     /translation="MQFELELLSPANADAPRVVASAVLRKHRLRNDVLRRVDYVRRFF
                     PELEGETITVGLTRAASGMAVPGGSRIWLNPSRLSHHTIAHELIHLLQLRNLGIPSGE
                     RACDVFSLARHWSLNDDRPSYVRVPLSLLDGSSAVSEAAARMLFDVARDALARRADGH
                     RRYLAYFETEVERRSPALASRENRASAAISGRLLT"
     gene            complement(3856..>4618)
                     /locus_tag="VEC56_05480"
     CDS             complement(3856..>4618)
                     /locus_tag="VEC56_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13639.1"
                     /translation="VRFDPSGQVLWGERFGDAGADDAVGIGVDAAGDLVLTGRFAGAI
                     DFGGAPLYGSSSNVYLAKLGEGSMEPILSITVLPRAGAIEAQWDISSRWTLERFFVLR
                     DNSSRTEPKIVAAGTLGPSSGVLMDRDVAPGETYRYELVVTTPTGVQFRSPVVTTTVP
                     VYTTQLSQNFPNPFNPRTSISYVLAARADVALVIFDVSGTLVRRIDQGPREAGEYVVE
                     WDGRDDAGNAVGSGVYFYRLEGISGVAPRKMVLLK"
BASE COUNT          785 a         1611 c         1488 g          734 t
ORIGIN      
        1 tgctaaacga gatcgacgcg ttccacccgc ccgtcgtcga ctccgcgcag acctgggccg
       61 agtggcacta tttcaactac cacgacgacg cagggcgttc attatatgtg tcgatcagcg
      121 ggatcgccac cgagggcgac ctcgcgccca gcggctactc gcgcgcggtg gttctgttcg
      181 agatggtcga ccacaccggc gcgcgcacca agatcgtcga cgtgctgccg ggccgcgaga
      241 tcgcggcttc gcaccgctcg gccgacctcg tggccggttt gaacacggtg catctcgagg
      301 ccggcgtgta ccgcgtgcac ctggccctcg ccgactcact cgcgcggccc atcacggtgg
      361 tcctcgaatt gacgcccacg cctcaccagg tcatcccgcc catcacgccc atgggctcgg
      421 aggttccctt cggctacgtc gtcccggtgg tgcgcggtca catgcgcggg cgcgtcacca
      481 tcggcggcac gacgtttccg ctcgacggtg tgggttacca cgaccacaac tgggggcatt
      541 tccgcgacgc ggtgtgggac tggggcatcg tgcacgcggg cgaggtgagc gtcctctacg
      601 ggcgtttcgc gacctcgatg gacgcgctcg cgaaccagcc cgtcctcttc gcgctcttcg
      661 acgcagcggg gccgcgaccg ttcgtcttga cccacgacta caccgtactg tggaggccgg
      721 tcgaggcgcg gcgccccagc ggaccgccgc gcacgcgcat cacgcagaac acgaccgcga
      781 gcgatacgcc gtcgtccgag cccgcaagcg tgctgttgag cgcggtctcg ggcgacgatt
      841 cgctttcgat ccgcatcgac gtcgaccggc gcttcgtcgc cgagacgggt ggcggcgaac
      901 agccgctgtt ccgcgccgac gaaacgcggc gctctttctt cttccaactg gagggacgcg
      961 cgcgcttgac gggtcgtgtc ggcggcgagc gcttcaacgc cgaaggccac gcgtactcgg
     1021 agacgtttcg ggtggacgct acggcgccac gacggtaggt tcctcgatcc cgttcatcgc
     1081 gtcgaccacg cgcagcaagt cgccggtccc atagaaatcg accgcgatca agttgggcac
     1141 catgcggcgc tcgcgcttgc acgcccgcgc ccgcttgagc aggaagtcgt aagcgttgac
     1201 cacctcggcg ttgctcggct tgggcgcggg tgtggtctcg atccagtggt tcatgaggag
     1261 caacgagcct gacgtgcccc cgcggcccgg cttgtttgag aactcagccg gatccaggaa
     1321 gcggtacggg gtttcctgga atacctccca tacgtggtgg taccagggca cgccctccgc
     1381 gttgttctcc gcgaacacca gcacgcgctg gtcgccgtcg atcatctcgc gcagcgtcgg
     1441 ccagggcggt gtgacgctgc cttggtacac gaactccacg agtccgctct tctcgaagca
     1501 cgtcgcgacg tcggcgggcg cgacaccttc gtcctgaatg acgatgatca ggacctcgcg
     1561 cgggtttgcg accagaaagc cgcgcacgcc ttctagcgcg tcgaagaaac gctcggcacc
     1621 cagctcgcag aacccgtggc agagatagac ctcgcggtcg ccgctgggct cgcccaccag
     1681 ccgatcgcgg atgcgcatgg cggcgtccac gccctccgct cccagcgcct ccgtgtattt
     1741 ctccatcgac gctttctcgt cctcgagctc ggtgcggatg cgatcgccca ccggcacacc
     1801 gaagtgaatg tcgatcaaga agccgcgcac gccttcttcg agctgggcgc ggagaccgcg
     1861 ttcctgattg ggaaacatcc agtcggaaat gtcggccgcc gccatcgagt tgtgggtggt
     1921 cgcgaaggcg acgtcgttca atcgcttgtc gcacaactcc gggtggagat tgcacgggtc
     1981 gagggccgac ccggccgccg cgaccacacc gtcgtcgtcg cgcgagagcc agtatgctcc
     2041 cgctcccgca agcgccagca ccaagccgcc gaccacggcc acgcgcggcc acgcggatcg
     2101 cttgcgctcc gtcgctgctt cgatcgcggc ctgcgcctgc gggaagaagc gcgccgcgat
     2161 cgtgaacact tcctggatgc cgacgaagaa gagcgcgccg ccggcgatga cgaagatcac
     2221 gtcgatcgcg tgggagggaa acaggaccac cagcatcccg gcacaaatga agaggagggc
     2281 gcggggaaac gcccagcgcg agcgaagcgg gcgagtctcg atgcggcgcc acagcgtgcg
     2341 cgcgagcgac tccaggtcga ccttctgcaa cagcgacgtc acgcccgcca ccagcaccag
     2401 accgatggcg gcgagaatca gcatgcggag cgcgagcggg ccgacgaaga cgggccacat
     2461 cccgcgcact aggtccgcga cgacgggcga ttgcgcgaat gcagccatga cttccccgcc
     2521 gaaacgggcg accgccccga gcaccaacga cgtaatggtc aggccgattc ccacccggag
     2581 caggtagcga tctcggcgac gcgtgagcca cagcccgcac acgcccgccg cgaagccgac
     2641 gctcagccag accacggagc gcacgcgcag gcggtggcca atacgaaggg cgcgctgcag
     2701 cagcttgccg ttgggccagt cctccggcga acccagcacc agctgcgctc tctcggggat
     2761 tttttccgcg atctcggggt acatggcgag cgcgttgcgg acgacgacgc ccaggtcggc
     2821 ggcggccagc gccaggtcct caccggtttc cgaaatgagg gtggcgtggg ccctcttggc
     2881 cgcgcggcga atcagcgcgc gaaacggcgc cgacgacacc acgtattcga ccgtgccgag
     2941 aatgatgggc cggtacgcgg tcaggtcgcg gcgagcctca atgatctggt cggtgacctc
     3001 ggccgccacg acgcgcgcaa ccgcgggctc ggcgaggcta tcggcggcgc gctgcgagaa
     3061 tcgcgacgcg tcgaagaggg tgcgcgaggt gtacgtgaac acgacgccga acaccagtcc
     3121 ggcgacgcac agcaccccgc agacaacggc gagcgtggct cgcatctttc ccatgacgag
     3181 ccgatgctat gccggtagcg cgtcgttcgc aacgcaaaat tgttggccac gtgtcgggcc
     3241 ccgtgctacc gtcgcgcgcg aaacgaggcc gccgcgtgca attcgagctc gaacttcttt
     3301 cccccgcaaa cgccgacgcg ccccgcgtgg tggccagtgc cgtgcttcgc aagcaccgcc
     3361 tgcgcaacga cgtgttacga cgcgtcgact acgtgcgccg attctttccc gagttggagg
     3421 gcgagaccat cacggtcgga ctcacgcggg ccgcgagcgg gatggcggtt ccgggcggtt
     3481 cgcgtatctg gttgaacccg tcgcggctct cgcaccacac gattgcgcac gagctgattc
     3541 acctgctgca gctgcgcaat ctgggcattc catcgggcga acgcgcgtgc gacgtgttct
     3601 cgctggccag gcattggtcg ctcaacgacg accgcccgag ctatgtccgc gtgccgcttt
     3661 cgctgctcga cggcagcagc gcggtgtcgg aggccgccgc gcgcatgctc ttcgacgtgg
     3721 cgcgggacgc cctcgcccgc cgcgccgacg gacaccgtcg ctatcttgcg tacttcgaaa
     3781 ccgaggtgga acgaagaagc ccggccctcg cgtcgcgcga gaaccgggct tcggcggcaa
     3841 tctcggggcg tttgcttact tgagcagcac catcttgcgc ggcgcgacac cgctgatgcc
     3901 ttcgagccga tagaagtaca ccccactccc caccgcgttg cccgcgtcgt cgcggccgtc
     3961 ccattcgacc acgtactccc ccgcctcccg cggcccttgg tcgatgcggc gcaccaaggt
     4021 tcccgagaca tcgaagatca cgagtgccac gtccgcccgc gccgccagca catacgagat
     4081 gctggttcgc gggttgaagg gattggggaa attctgcgac agctgagtcg tgtataccgg
     4141 caccgtcgtc gtcacgacgg gagagcgaaa ctgaacgccc gtcggcgtcg tcacgacgag
     4201 ctcgtaccgg taggtctcgc ccggcgcgac gtctctgtcc atgagcacgc cactactcgg
     4261 tccaagagtc cccgctgcaa cgatcttggg ctctgtgcgc gaactattgt cgcgcagcac
     4321 gaagaagcgc tcgagcgtcc accggctcga gatatcccac tgcgcttcga tcgctccggc
     4381 acgcggcagg actgtgatcg aaagtatcgg ctccatcgaa ccctcgccca gcttggcgag
     4441 gtatacattg gaactcgatc catagagcgg ggctcctcca aagtcgatcg cgccggcgaa
     4501 tcttcccgtg agcacgagat ccccagccgc atcgacgccg atcccgaccg catcgtcggc
     4561 gcctgcgtcg ccgaatctct caccccagag cacttggccg ctcggatcga agcggact
//
LOCUS       DASPGK010000194         7482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_518483, whole genome shotgun sequence.
ACCESSION   DASPGK010000194 DASPGK010000000
VERSION     DASPGK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518483"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VEC56_05485"
     CDS             <1..1188
                     /locus_tag="VEC56_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXV13640.1"
                     /translation="TDSTPARKLIVAVAGMSSLLTRELCAQIGTLDSLLEHGDRVDET
                     LTTIAEWERFSAQDAARRGVAAEERRMREREWFERLRLRTFAECVRRGFGPGPHGATG
                     ELSRATAARLHLIAAFDEMIPEREHVAVFAMGSYAVGEPRITSDVDLVVVADDVDLPD
                     LTQRLQVINQWFSDGRILKLDFRLRAEGTSSPLVQDISFYEEYFRSRASLWERAAFAK
                     ASAWWGGEEVRRRFLRLLRSFAAKSFTPGEVTQLVEMRKKVESLAPKQFVEWDTKRSA
                     GGRYDIEYLCAIGLAPGAKDRLDYFTMSTRERVAALAEAGVVSEAESAMLHEAMDLFG
                     LVEHLMDLQEMTHPSSEEKAEYLASYLDRTFEHLGLSHGSTRARLAAAKKSVRECFAR
                     SAR"
     gene            complement(1207..3882)
                     /gene="glnD"
                     /locus_tag="VEC56_05490"
     CDS             complement(1207..3882)
                     /gene="glnD"
                     /locus_tag="VEC56_05490"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXV13641.1"
                     /translation="MTTRATKTRRPPRPRAASAHALPSPVSPHAAASREQFVAETKAY
                     LAILTKPVLDMRTPKTGGREFVSSYTRMVDTIVGLLFQRAAEDNGRRADDTDIAVIAM
                     GGYGRAELAPYSDVDILIACGKKSPLVEAIAGSFVRLLWDTGFETGHAVESLVEADSA
                     LTRDIDTKTALIESRWVCGSKRVAKALEKKIGRIRKSDREEYLRRKIDDALARREKFG
                     NSFQLIEPNVKLSPGGLRDYQTLVWLGLVGRAPHGLQALRNKGLLLRGERRELDEAYD
                     FLLRVRVEMHVATRSKQDQLMVAVQQDMAKRLGFAPKGDHLAVEFFMREYYRHTRSIA
                     RITNDCIDALGRGTDLGVLLGQGRLKQDGSKLDMPLRLPTLRKHPLSVFERQKASGQK
                     LSRALRRRLEHVVQEDLGAAPIVERMRRSFPSFLDDDRNVDLVVRSLHETRFLMKIIP
                     EYDQLTCLKRYDLYHHYTVDEHSFKVLENLVALGRAGASAHDPLVRLYSELPEKRVLF
                     LAALLHDIGKIEGRGHAKKGAVLSRTILKRMAVPQHEIDIISDLIENHLLMSHFSQRR
                     DPTDIGTITAFCDRVGNRTRLKNLCLLTYADYRATSPLVWNEWKRTLLWELYVRAYDF
                     MARREKQPEDVYRQHKEHLLATFPEGDARRRALSHLDLLPGGYLLTMTPEMVRDHIQM
                     IERLNGEPVVVAHRFVGATHEITFCTHDKPYRLSELCGVLAINDFTILNAFAFTRRDG
                     KVIDVFVVEPVDRGEALEPDETLQRVERIRDQLLGVFHGTLDLAAATQKHAHRWRRVA
                     KGGIPVATQVTFENDVSDEFTIIDVFAQDRPGLLYTITRTLSQRGLTIARAKISTEGA
                     RAIDSFYVRGEEGKVTAPVKLEETRAALGTAIDDR"
     gene            complement(3980..5401)
                     /gene="glnA"
                     /locus_tag="VEC56_05495"
     CDS             complement(3980..5401)
                     /gene="glnA"
                     /locus_tag="VEC56_05495"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXV13642.1"
                     /translation="MEKRSPSEVLKFAKEKGAKVLDLKFVDMPGTWQHFSLPIHALEE
                     KSFEDGFGFDGSSIRGWKHINESDMLVIPDPDTAFVDPFTKETTLSLVCNIFDPITKE
                     RYSRDPRGIANKAEAYVKSTGLATDIYFGPEAEFFIFDGIRYEAQPQQSFFYIDSAEG
                     AWNTGRDEQPNLGFKLRFKEGYFPVPPADSLQDIRTEMMLEIMKMGIEVEAQHHEVAT
                     AGQSEIDLKFDTLVRMADCLMTYKYVIRNVAKRHGKTVTFMPKPLYGDNGSGMHCHQS
                     LWKGNTPLFAGNKYAGLSDMALHYIGGLLKHAPALCAFTNPTTNSYKRLVPGYEAPVN
                     LAYSQRNRSASVRIPMYSQSPKAKRVEFRCPDPTCNPYLGFAAMVMAGLDGIQNRLSP
                     GEPLDKNIYDLPPEELAKVPQAPGSLNQSLDALEKDHDFLLKGDVFTKDVIETWIDYK
                     REKEVGPMSLRPHPYEFFLYYDA"
     gene            complement(5553..7106)
                     /locus_tag="VEC56_05500"
     CDS             complement(5553..7106)
                     /locus_tag="VEC56_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXV13643.1"
                     /translation="MRVLTSEQMQKVDAETIARVVPGIELMERAGRGVAKGIAARVER
                     GKAVIFVGPGNNGGDGLVVARLLAAAGWECSIHLLKTTDLTPDTAKNYQRLNKKDFHE
                     FDASRPDWPKRAPDDLADAAVIVDAIFGTGFEGAPRGRAAEMIALINAASALRKTPVF
                     AIDIPSGVDGTTGEVAGEAVRAYTTITIGAPKTGLLFYPGKEYVGALTVIDIGFPSEI
                     VEKHANPWFYLDADAAAQMLPKRAPNIHKYGAGTLLLIAGSEAYRGAALLAGEAALRG
                     GCGMVYLAVPENIRAEVSVALREAVTVALPQTPQGTIAKGAAAALRDTVAKANAIAIG
                     PGLGRSDETDEFVRDFVVHTTKPCVVDADALTAFTGHAPDLADRKRVITPHDGELARL
                     VGELRAANPVERLDETAGISKRLQVTLVRKGAPSLVAVPDGRIWINSSGSSALATGGT
                     GDVLTGLVGSLLAQGAEPMDAACVACFLHGRAGELAALEKGLRGVIAGDLIPLLGPAM
                     RALEGRAGR"
     gene            complement(7106..>7482)
                     /locus_tag="VEC56_05505"
     CDS             complement(7106..>7482)
                     /locus_tag="VEC56_05505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV13644.1"
                     /translation="ELMKRGIKLGVVSDAPRKEAWLRLCYLQLHHMFDFVTTFEDTGV
                     RKPSPKPFQRALDYFQLQPQQAIMVGDWPERDITGAGQLGLVTVFARYGDTKGAKESG
                     ANHDINDVFELVDIVDKLNAGA"
BASE COUNT         1207 a         2427 c         2503 g         1345 t
ORIGIN      
        1 accgactcga cgcccgcgcg aaagctgatc gtcgccgtgg ccgggatgtc gtctttgttg
       61 acgcgcgagt tgtgcgcgca gatcgggacc ctcgactcgc tgctcgagca cggcgaccgc
      121 gtcgacgaga cactaacgac catcgcggag tgggagcgct tctcggccca ggacgccgcc
      181 cggcgcggag tcgcggccga ggagcggcgc atgcgcgagc gcgagtggtt cgagcgcttg
      241 cggttgcgca cctttgccga gtgcgtgcgg cgggggttcg gtcccggacc gcacggcgcc
      301 accggcgaac tctcgcgcgc gacggccgca cgcctccact tgatcgcggc cttcgacgag
      361 atgatccccg agcgcgagca cgtggcggtg ttcgctatgg gatcgtatgc ggtgggcgag
      421 ccgcgcatca ccagcgacgt cgacctggtc gtggtcgccg acgacgtcga tctgcccgac
      481 ttgacgcagc gactgcaggt catcaaccag tggttctccg acggccgcat cttgaagctc
      541 gacttccgtc tgcgcgccga gggcacgagc tcaccgctcg tccaggacat ttcgttctac
      601 gaggagtact ttcgctcgcg cgcgtccttg tgggagcggg ccgcgttcgc caaggcctcg
      661 gcgtggtggg gcggcgagga ggttcggcgg cggttcctgc gcttgctgcg ctcgttcgcg
      721 gccaaatcat tcacgcccgg cgaggtgacg caactggtcg agatgcgtaa gaaagtcgag
      781 tcgcttgcgc ccaagcaatt cgtcgagtgg gacaccaagc gctccgcggg cggccgttac
      841 gacatcgaat atctgtgcgc gatcggactg gcgcccggcg ccaaggaccg tttggactac
      901 ttcacgatgt cgacgcgcga gcgcgtcgca gcgctcgcgg aagccggtgt cgtctccgag
      961 gccgagagcg cgatgttgca cgaggcgatg gacctgttcg gcctggtcga gcacctgatg
     1021 gacttgcaag aaatgacgca cccgtcgagc gaggaaaagg ccgagtatct ggcgagctac
     1081 ctggaccgga ccttcgagca cctcgggctt tcgcacgggt cgacgcgcgc ccgtctcgcc
     1141 gcagccaaga agtcggtccg tgagtgcttc gcgcgcagcg cgcgctagga ccgctgcgtt
     1201 ttaaaattac cggtcgtcga tcgcggtccc gagcgccgcg cgcgtttctt ccagcttgac
     1261 cggcgcggtg accttcccct cctcaccccg cacatagaac gagtcgatcg cccgtgcgcc
     1321 ttcggtggag atcttggcgc gcgcgatggt gagaccgcgc tgcgagagcg tgcgcgtgat
     1381 cgtatagagc aatccggggc ggtcctgcgc gaacacgtcg atgatggtga actcgtcgga
     1441 gacgtcgttc tcgaacgtca cctgggtggc cacggggatg ccgcccttgg ccacgcggcg
     1501 ccagcggtgg gcgtgcttct gggtggcggc cgcgaggtcg agcgtgccgt ggaacacgcc
     1561 gagcaattga tcgcggatgc gctccacgcg ctggagggtc tcgtcgggct ccagcgcttc
     1621 gccgcggtcg accggctcca ccacgaacac gtcgatcacc ttgccgtcgc ggcgggtgaa
     1681 ggcgaacgcg ttcaagatgg tgaagtcgtt gatggcgagg acgccgcaca actcggagag
     1741 acgatagggc ttgtcgtgcg tgcagaacgt gatctcgtgg gtggcaccga cgaagcggtg
     1801 cgccaccacc accggttcgc cgttcaatcg ctcgatcatc tggatgtggt cgcgcaccat
     1861 ctcgggcgtc atcgtgagca gatagccgcc cggcaagaga tcgaggtgcg acaaggcgcg
     1921 acggcgcgcg tcgccctcgg ggaaggtcgc cagcaggtgc tccttgtgct ggcggtacac
     1981 gtcctcgggc tgcttctcgc gccgcgccat gaagtcgtac gcgcgcacgt acaactccca
     2041 gagcagtgtg cgcttccatt cgttccacac cagcggcgag gtcgcgcggt agtcggcgta
     2101 cgtgagcaga cagaggttct tcaagcgcgt gcggttgccc acgcggtcgc agaaggcggt
     2161 gatggtgccg atgtcggtgg ggtcgcggcg ctggctgaag tgcgacatca agagatggtt
     2221 ctcgatgaga tcgctgatga tgtcgatctc gtgctggggg accgccatgc gcttcaagat
     2281 ggtgcgcgag aggacggcac ccttcttggc gtggccgcgc ccctcgatct tgccgatgtc
     2341 gtggaggagc gcggccagga acagcacgcg cttctccggc agctcggagt agaggcgcac
     2401 gagcgggtcg tgcgcgctcg cgccggcgcg gcccagcgcc actaagttct ccagcacctt
     2461 gaagctgtgc tcgtcgacgg tatagtggtg atagaggtcg tagcgcttca agcacgtcag
     2521 ctggtcgtac tcggggatga tcttcatgag gaagcgcgtc tcgtggagcg agcgcacgac
     2581 caggtccaca ttacgatcgt cgtccagaaa cgaagggaac gagcgccgca tgcgctcgac
     2641 gattggcgcc gcgcccaagt cttcttgcac gacgtgctcc aggcggcggc gcagcgcgcg
     2701 cgacagcttc tgcccgctcg ccttctgcct ctcgaacacg gacagcgggt gcttgcgaag
     2761 tgtgggcagc cgcaacggca tgtcgagctt cgacccgtcc tgcttgaggc ggccctgacc
     2821 cagcagcacc cccaagtcgg tgccgcggcc gagcgcgtcg atgcagtcgt tggtgatgcg
     2881 cgcgatggag cgcgtgtggc ggtagtactc gcgcatgaag aactcgaccg cgaggtggtc
     2941 gcccttgggc gcgaagccca gcctcttcgc catgtcctgc tgcaccgcga ccatcagctg
     3001 atcctgtttg gaacgcgtgg cgacgtgcat ctcgacccgc acgcgcagca agaaatcgta
     3061 ggcttcgtcc agctcgcgac gctccccgcg caggagcagg cccttgttgc ggagcgcttg
     3121 cagcccgtgc ggagcgcgcc ctaccaggcc caaccacacc agcgtttgat aatcgcgcag
     3181 tccgccgggg ctcagcttca cgttgggctc gatcagctgg aagctgttgc cgaacttctc
     3241 gcggcgcgcg agggcgtcgt cgatcttgcg gcgcaggtac tcctcgcggt cgctcttgcg
     3301 aatgcggccg atcttcttct cgagtgcctt ggcgacgcgc ttcgacccgc acacccaccg
     3361 gctctcgatg agcgcggttt tggtatcgat gtcgcgcgtc aaggccgagt ccgcttcgac
     3421 cagcgactcc accgcgtggc cggtctcgaa gcccgtgtcc cacaggaggc gcacgaacga
     3481 ccccgcgatg gcttcgacca acggggactt cttgccgcac gcgatcagga tgtcgacgtc
     3541 ggagtaaggc gccagctccg cgcgcccgta gccgcccatc gcgatcaccg cgatgtcggt
     3601 gtcgtcggcg cggcggccgt tgtcctcggc ggcgcgctgg aacagcaacc ccacgatggt
     3661 gtcgaccatg cgcgtgtagc tcgatacgaa ctcgcgccca cccgtcttgg gcgtgcgcat
     3721 gtcgagcacg ggcttggtga gaatggcgag atacgccttg gtctccgcga cgaactgctc
     3781 gcgcgaggcg gccgcgtgcg gcgaaaccgg cgacgggagc gcgtgcgcgg aagccgcgcg
     3841 cggacgcggc gggcggcggg tcttggtggc gcgcgtggtc acgcaggcat tctaagccat
     3901 cgcgacgaaa acacgaaggc ccggctcgct tgcgcgaacc gggcctccgc ttcagtcgcc
     3961 cccaccagag gcgaacacac tacgcgtcgt aatacaggaa gaactcgtac gggtgcgggc
     4021 ggagcgacat cggccccact tccttctccc gcttgtagtc gatccacgtc tcgatgacgt
     4081 ccttcgtgaa gacgtcgccc ttcaacaaga agtcgtggtc cttctccagc gcgtcgagcg
     4141 actggttcaa ggacccgggc gcctgcggca ccttggccag ctcctcgggc gggagatcgt
     4201 agatgttctt gtcgagcggt tcgcccgggc tcaagcggtt ctggatgccg tccagccccg
     4261 ccatcaccat cgcggcgaaa cccaggtacg ggttgcaggt cggatccggg cagcggaact
     4321 cgacgcgctt cgccttgggc gactgcgagt acatggggat gcgcaccgag gcgctgcggt
     4381 tgcgctgcga gtacgcgagg ttgaccggcg cttcgtatcc ggggaccaag cgcttgtagg
     4441 agttggtggt cgggttggtg aacgcgcaca aggccggcgc gtgcttgagc aatccgccga
     4501 tgtagtgaag cgccatgtcg ctcaagcccg cgtacttgtt gcccgcgaag agcggcgtgt
     4561 tgcccttcca caaactctgg tggcagtgca tcccggagcc gttgtccccg tagagcggct
     4621 tgggcatgaa cgtgaccgtc ttgccgtggc gcttggcgac gttgcgaatg acgtacttgt
     4681 acgtcatcag gcagtccgcc atgcgcacca gcgtgtcgaa cttgagatcg atctccgact
     4741 gccccgccgt cgccacctcg tggtgctgcg cttccacctc gatgcccatc ttcatgatct
     4801 cgagcatcat ctccgtgcga atgtcctgga gcgagtcggc gggcggaacc gggaagtagc
     4861 cttccttgaa gcgaagcttg aagcccaggt tgggctgttc gtcgcggccg gtgttccagg
     4921 caccctcggc cgagtcgata tagaagaacg attgctgcgg ctgcgcctcg tagcgaatgc
     4981 cgtcgaagat gaagaactcg gcttcggggc cgaagtagat gtcggtggcg aggcccgtgc
     5041 tcttcacgta ggcctcggct ttgttggcga tcccgcgcgg gtcgcgcgag tagcgctcct
     5101 tggtaatggg atcgaaaatg ttacagacca gggagagcgt ggtctctttg gtgaacgggt
     5161 cgacgaacgc ggtgtcggga tccggaatca cgagcatgtc gctctcgttg atgtgcttcc
     5221 aaccgcggat gctggatccg tcgaagccga agccgtcttc gaacgacttc tcctcgagcg
     5281 cgtgaatggg caggctgaag tgctgccacg tgccgggcat gtcgacgaac ttgagatcga
     5341 gtaccttcgc acctttttct ttggcgaatt tgagaacttc cgaaggagat cgcttctcca
     5401 tcacaccacc ctcctggacg tagattgcgc ggggtcgacg gaacgcccac tggcgaagcc
     5461 gtcgatttga aagaggaaca tttaagcccg cgcgcagggg gtgtcaacgg cgaacccggg
     5521 ggcgcggtag aaaaggtgtg gacggcgccg cgctaccggc cggcgcgtcc ttcgagggcg
     5581 cgcatcgcgg ggcccagcaa ggggatgagg tcgccggcga tgacgccgcg gaggcccttc
     5641 tccagcgcgg ccaactcgcc cgcgcgcccg tggaggaagc acgcaacgca tgctgcatcc
     5701 atgggttccg cgccctgggc gaggaggctc ccgaccaggc cggtcagtac gtcgccggtt
     5761 ccgcccgtcg ccagggccga cgatccggag gagttgatcc atattctccc atccggaacg
     5821 gccaccaggc tgggggcacc cttgcggacc agcgtgacct ggaggcgctt cgaaatgccg
     5881 gccgtttcgt ccaggcgctc gaccgggttg gccgcgcgca actcgccaac caagcgtgcg
     5941 agctcgccgt cgtgcggcgt gatgacgcgc tttcggtccg cgagatccgg cgcgtggccg
     6001 gtgaacgcgg ttagagcgtc ggcgtcgacc acgcacggct tggtcgtgtg cacgacgaag
     6061 tcgcgcacga attcgtccgt ctcgtcgctg cgacccaacc ccggcccgat cgcgatcgcg
     6121 ttcgccttgg cgacagtgtc gcgcagcgcc gccgctgccc ccttcgcgat cgtcccctgc
     6181 ggcgtttgcg gaagtgcgac cgtcaccgct tcgcgcagtg cgaccgagac ttcggcgcga
     6241 atgttttcgg gcaccgcgag gtagaccatg ccgcatccac cgcgcagcgc tgcttcgccc
     6301 gccagcagcg ccgcgccgcg atacgcttcg ctgcccgcga tcaagagcaa cgtgcccgcg
     6361 ccgtacttgt ggatgttggg cgcgcgcttc gggagcattt gcgcggccgc gtccgcgtcc
     6421 agatagaacc atgggttcgc atgcttctcg acgatctcgc tcgggaatcc gatgtcgatc
     6481 accgtgagcg cgcccacgta ctccttgccc ggatagaaca gaagccccgt cttcggcgca
     6541 ccgatcgtga tcgtcgtgta cgcgcgcacc gcttcgcccg ccacttcgcc cgtggttccg
     6601 tcgacaccgg aaggaatgtc gatcgcgaag accggcgttt ttcgaagcgc ggacgccgcg
     6661 ttgatcagcg caatcatctc cgccgcacga ccgcgcggag ctccttcgaa gcccgtccca
     6721 aagatagcgt ccacaatcac cgccgcgtcc gccaaatcgt ccggtgcgcg cttcggccaa
     6781 tccggtcgcg acgcatcgaa ctcgtggaag tccttcttat tgaggcgttg gtagttcttc
     6841 gccgtgtccg gcgtcaggtc ggtcgtcttc aagagatgga tcgagcactc ccaacccgcc
     6901 gcggccaaga gccgcgccac caccaaccca tcccccccat tgttccccgg ccccacgaag
     6961 atgaccgcct tcccccgctc cacgcgcgcc gcgatcccct tcgccacccc gcgcccggcg
     7021 cgctccatca actcgatccc cggcaccaca cgcgcgatag tctcggcgtc gaccttctgc
     7081 atttgttcgg aggtgagaac gcgcatcaag ctcccgcatt cagcttgtcc acaatatcca
     7141 ccagctcgaa gacgtcgttg atgtcgtggt tggcgccgct ttccttggca cctttggtgt
     7201 cgccgtagcg ggcgaagacg gtgaccaggc cgagttggcc ggcgccggtg atgtcgcgct
     7261 cgggccagtc gcccaccatg atcgcttgtt gcggctggag ctggaagtaa tcgagcgcgc
     7321 gttggaaggg cttggggctg ggcttgcgca cgccggtgtc ctcgaaggtg gtgacgaagt
     7381 cgaacatgtg gtggagctgg aggtagcaca agcgcagcca cgcttccttg cgcggtgcgt
     7441 cggagacgac accgagcttg atgccgcgct tcatgagttc ga
//
LOCUS       DASPGK010000195         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1104663, whole genome shotgun sequence.
ACCESSION   DASPGK010000195 DASPGK010000000
VERSION     DASPGK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104663"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VEC56_05510"
     CDS             complement(<1..764)
                     /locus_tag="VEC56_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HXV13645.1"
                     /translation="MKFAAVDIGSNAVRLLLARVIEESDEPLFKKEVLVRMPLRLGDD
                     AFRTGAISEEKAQRLVETMVGFRHLIAAYPALDFAAYGTSAMREAKNVRDVVARVRKA
                     SGISIQVIEGAREAEVIYANHIEHRLDPRRNYLYVDVGGGSTEVTLIARGRKVGSSSF
                     PVGTVRMLQGRPNQRALDGLRSWVKRHRTEHGSMTAIGSGGNINTIFKLARAKKDRPL
                     SYAQVRDIRRMVKSYSLEERIVTLGLRPDRADVIVPA"
     gene            complement(761..1573)
                     /locus_tag="VEC56_05515"
     CDS             complement(761..1573)
                     /locus_tag="VEC56_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV13646.1"
                     /translation="MKRIILVRHATAVDVGPRGSDFQRRLKKRGRREAAIMADRVASS
                     LAAPDLAVTSPAARAIETARIFAERLGLSAERVVAREQLYGGLLPDEFLRIVREMDDA
                     HDALMIFGHDPSFSEFAAYLVPGFTAFIPKAGVVAIDLDRASWSAVRAGDGKLVVFER
                     PPAPEVQKKIEEDLIDRLAMEIRTAVFASLRSLGVPENRDVVKAVARASSRLALVARP
                     FAAAASPAAPKGRKRSPSSKRVLTKRRAKRVAKGPRRSSSASVAKRRARSRA"
     gene            complement(1589..3127)
                     /locus_tag="VEC56_05520"
     CDS             complement(1589..3127)
                     /locus_tag="VEC56_05520"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXV13647.1"
                     /translation="MREERLFVAGRGRGWRDARRALARAFSLAPEPGARDLRTYHDTF
                     DWRFYRAGLQLETSDARVALRDATTAEVLASGSWRGPAGVRYARDLVPPALRDRVAAL
                     AGPRALFEVARARVTVDEAAARDERGRQVARLRSETMIVRRSGAARTVRVLFVEIAKE
                     RTRLETRASIRRALGSAGYRPSSGSAYGRLLSAAGRRPDDDAPRPRVEISPQAPLGAS
                     MAAILRHLLDVMARNESGLVRDLDPEFLHDYRVALRRTRTALAEFRGVFTAEEARAFR
                     SRFAALSGPTGRVRDVDVHLGRRAEYAAWVPAPFRKGFAPLFASLEREREARRAGLLT
                     LLASPEYAALKRDWKRALAALASGSRAGVAAGEPALPAARAAVAKRYARLRAIAEALE
                     DLDDAALHRARVQSKRLRYVLEFFTHPLGGAVLPAIAAVESLQDALGAYHDAGVFQSL
                     LGEEAHRSSSISRDAVVHGAVIGALTARLDVRKAKARRRAVRRLDALVDRKGERMFRR
                     LAES"
     gene            complement(3120..>5171)
                     /gene="ppk1"
                     /locus_tag="VEC56_05525"
     CDS             complement(3120..>5171)
                     /gene="ppk1"
                     /locus_tag="VEC56_05525"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXV13648.1"
                     /translation="INREISWLSFNDRVLQEASDPSVPLVERLKFIGIFSSNLEEFFR
                     VRVATLQRMVDAGIDSNKMVYGGSPKKVLKQIHEVVVEQRGRFDRVFAEVRAALEKEN
                     VFLIDERQLLPEHREFVQGYFEDSVRPTLVPVILDTLPEFPYLKNGVIYLAVRLESKQ
                     NGAKPRHALIEVSTDTLPRFLVLPGTGEKRYVIMLDDVIRFGLKEVFSIFDVDRADAY
                     TIKLTRDAEIEIDDDVTMSLMDKISKSLKKRKEGPPVRFVYDRELPKDLLDLIMKKAN
                     LRKLDNVIAGGRYHNARDFVSFPKVTGSSLQYEPQPPLHHPALATTRSVFSVMRKQDV
                     LLHLPYQSFHHVTDLLREAAIDPKVKSISMTLYRVARDSHVVNALINAVRNGKKVTVL
                     FELQARFDEEANIRWTRKLEDEGARLIGGVPGLKVHAKLALITRKEEGKLVDYALVGT
                     GNFNEVTARVYADHILMTSDERVTVELRKLFDFLETNYRTHQYHGLMVSPFDMRRVLH
                     KLVRREIKNARAGKPAYIDVKLNSLVDRELIYLLYEASRAGVKVRLIVRGTCSLVPGL
                     PGVSENIEAVSIVDRYLEHSRIFFFANGGNERCYLSSGDWMTRNLDFRVEIAVPVYDD
                     AVRAELRYYFNLQLRDTAKARIINQAQDNPYRRGSRNHRAQIDIYRWLARASGHRGSD
                     A"
BASE COUNT          815 a         1760 c         1750 g          846 t
ORIGIN      
        1 gcgggtacga tgacgtcggc gcggtcgggg cggaggccca gcgtcacgat gcgctcctcc
       61 agcgagtagg acttcaccat gcgccggatg tcgcgcactt gcgcgtacga caggggccgg
      121 tccttcttgg cccgcgcgag cttgaagatg gtgttgatgt tgccgccgct tccgatcgcg
      181 gtcatgctgc cgtgctccgt gcggtggcgc ttgacccacg agcgcagccc gtcgagcgcg
      241 cgctggttcg ggcggccctg cagcatgcga accgttccca ccgggaagga gctgctcccc
      301 actttgcggc cgcgcgcgat cagcgtcact tcggtgctcc cgcccccgac gtcgacgtag
      361 agatagtttc tgcgcggatc gagacggtgc tcgatgtggt tggcgtagat cacttccgcc
      421 tcgcgcgcgc cttcgatcac ctggatggag atgccggacg ccttgcgcac gcgcgccacc
      481 acgtcgcgca cgttcttggc ttcgcgcatc gcactggtgc cgtaggcggc gaagtcgagc
      541 gcggggtagg ccgcgatgag gtgtcgaaac cccaccatgg tctcgaccag acgctgcgcc
      601 ttttcttcgg agatcgcccc ggttcgaaag gcgtcgtcgc ccagacgcag cggcatgcgc
      661 accaggactt ccttcttgaa gagaggctcg tcggattcct cgattacgcg cgcgagcagg
      721 aggcgaaccg cgttgctgcc gatgtcaacc gcggcgaact tcatgcgcgc gacctggcgc
      781 gacgcttcgc gaccgacgcc gacgacgaac ggcgcggtcc cttggcgacc cgcttcgcgc
      841 gccgtttggt aaggacgcgc ttgctcgaag gcgaccgctt gcgccccttg ggcgccgccg
      901 gagacgctgc ggcggcgaag gggcgcgcca ccaacgccag gcgcgagctc gcgcgcgcca
      961 ccgccttgac cacgtcgcgg ttctccggca ccccgagcga gcgcagcgac gcgaacaccg
     1021 ccgtccgaat ctccatcgcc agccgatcga tgaggtcttc ctcgatcttc ttctggactt
     1081 cgggtgccgg cgggcgctcg aacacgacca actttccgtc gcccgcgcgc acggcactcc
     1141 acgaggcgcg atcgagatcg atcgccacca cacccgcttt gggaatgaag gcggtgaagc
     1201 ccggcaccag gtaggccgcg aactccgaga acgacgggtc gtgcccgaag atcatgagcg
     1261 cgtcgtgggc gtcatccatc tcgcgcacga tgcgcaggaa ctcgtcgggc aggagtccgc
     1321 cgtagagctg ctcgcgcgcg accacgcgct cggcggacag gcccagccgc tccgcgaaga
     1381 tacgcgcggt ctcgatcgcg cgcgcggcgg ggctggtcac cgcgagatcg ggtgcggcga
     1441 gcgagctcgc cacgcggtcc gccatgatgg cggcctcgcg ccgcccgcgt ttcttcaatc
     1501 gacgctggaa gtcggatccc cgcggcccca cgtcgaccgc ggtcgcgtgt cgaaccagga
     1561 tgattctctt cattgcaccc tcctgtcctt aggattccgc cagtcgccgg aacatgcgct
     1621 cgcccttgcg atcgacgagt gcgtccagcc ggcgcacggc gcgccggcgc gccttcgcct
     1681 tgcgaacgtc cagccgcgcg gtcagcgcgc cgatgacggc gccgtgcacg acggcgtcgc
     1741 gcgagatcga ggaggacctg tgcgcttcct cccccagcag cgactggaat acacccgcgt
     1801 cgtggtaggc cccgagtgcg tcttgcagac tctcgacggc cgcgatcgcg ggcagcaccg
     1861 ccccacccag cgggtgggtg aagaattcta gcacgtatcg aaggcgtttg ctctgcacgc
     1921 gcgcgcgatg gagcgccgcg tcgtcgaggt cctccagcgc ctcggcgatc gcacgcaggc
     1981 gcgcgtagcg cttggccacg gccgcgcgcg ccgccgggag cgcgggctcg ccggccgcga
     2041 cgcccgcccg ggatcccgac gcgagtgcag ccagggctcg cttccagtcg cgcttgagcg
     2101 ccgcgtactc gggactcgcg agcagggtca agagcccggc gcggcgcgcc tcacgctcgc
     2161 gctcgagcga ggcgaacagc ggcgcgaatc ccttgcggaa cggtgccgga acccacgcgg
     2221 cgtactctgc gcgacgcccg aggtggacgt ccacgtcccg cacgcgtccc gtggggccgg
     2281 aaagcgcggc gaaacgggag cgaaacgcgc gcgcttcttc cgcggtgaac acgccgcgga
     2341 actccgccag cgcggtgcgc gtgcgccgca gtgccacccg gtaatcgtgg aggaattcgg
     2401 ggtcgagatc gcgcacgagc ccggactcat tgcgcgccat gacgtcgagg aggtgtcgga
     2461 ggatcgcggc catcgacgcg cccagcggcg cttggggcga gatctcgacg cgcggtcgcg
     2521 gtgcgtcgtc gtcgggacga cgccccgccg cgctcagcag ccggccgtac gccgagcccg
     2581 aggaggggcg gtaccccgcc gacccgagag cacgtcgaat cgatgcgcgc gtttcaaggc
     2641 gcgttctttc cttcgcgatt tctacgaaca gcacgcgcac ggtgcgcgcg gccccgctcc
     2701 gtcgcacgat catcgtctcc gagcgaagac gcgcgacctg gcggccgcgt tcatcgcgcg
     2761 ccgccgcttc gtcgaccgtc actcgcgcgc gggccacttc gaacagcgcg cgcgggcccg
     2821 ccagcgcggc cacgcgatcg cgcagggcgg ggggaacgag gtcgcgcgcg taacggaccc
     2881 ccgctgggcc gcgccacgac ccggacgcga gcacctccgc ggtggtcgcg tcgcgcagcg
     2941 ctacccgggc gtcgctcgtc tccagctgca gccctgcgcg atagaaacgc caatcgaagg
     3001 tgtcgtggta ggtgcgcagg tcgcgtgcgc ccggctccgg cgccagcgaa aaggcacgcg
     3061 caagcgcacg ccgggcatcg cgccagccgc gaccgcggcc cgcgacgaag agccgctcct
     3121 cacgcatccg atccccggtg ccccgacgcg cgcgcgagcc accggtagat atcgatttgc
     3181 gctcgatggt tgcgagaccc gcgccggtac gggttgtcct gggcctggtt gatgatcctc
     3241 gccttggcgg tgtcgcgcaa ctgaaggttg aaatagtagc gcagctccgc gcgaaccgcg
     3301 tcgtcgtaga ccggcaccgc gatctcgacc cggaaatcga ggttgcgcgt catccagtcc
     3361 cccgagctca ggtagcagcg ctcgttgccg ccgttggcga agaagaagat gcgcgagtgc
     3421 tccaagtagc gatcgacgat gctgacggcc tcgatgttct cgctcacccc cggaagcccc
     3481 ggaaccagcg agcaggtccc gcgcacgatc aagcgcacct tcacgccggc gcggctggcc
     3541 tcgtaaagca gataaatcag ctcgcggtcg acgagcgagt tgagcttgac gtcgatgtag
     3601 gcgggcttgc ccgcgcgcgc gttcttgatc tcgcgccgga ccagcttgtg cagcacgcgc
     3661 cgcatgtcga agggcgacac catcagcccg tgatactgat gcgtgcggta gttggtctcg
     3721 aggaagtcga acagcttgcg cagttccacg gtgacgcgct cgtcgctcgt catcaggatg
     3781 tggtcggcgt acacgcgcgc ggtcacttcg ttgaagttgc cggtcccgac cagcgcgtag
     3841 tcgaccagct tcccttcctc cttgcgcgtg ataagggcca gcttggcgtg gaccttgagc
     3901 cccggcaccc cgccgatcag gcgcgcaccc tcgtcctcga gcttgcgcgt ccagcggatg
     3961 ttggcctcct cgtcgaagcg cgcctgcagt tcgaacagca cggtgacctt cttgccgttg
     4021 cgaacggcat tgatgagggc gttgaccacg tgcgagtcgc gcgcgacgcg gtacaacgtc
     4081 atgctgatcg acttcacctt gggatcgatg gccgcttcgc gcaggaggtc ggtcacgtgg
     4141 tggaacgact ggtacggcag gtgcaacagc acgtcctgct tgcgcatcac cgagaaaacg
     4201 ctgcgggtcg tggccagcgc ggggtggtga aggggcggct gcggctcgta ctgcaggctc
     4261 gaccccgtga ccttgggaaa cgacacgaag tcgcgcgcgt tgtggtagcg cccgcccgcg
     4321 atgacgttgt cgagcttgcg caggttggcc ttcttcatga tcaagtcgag gaggtccttg
     4381 ggcagctcgc ggtcgtacac gaagcggacg ggcgggccct ccttgcgctt cttcagcgac
     4441 ttggagatct tgtccatcag gctcatggta acgtcgtcat cgatctcgat ctcggcgtcg
     4501 cgcgtgagtt tgatggtgta ggcgtcggcg cggtcgacgt cgaagatgga gaacacttct
     4561 ttcaagccga agcggatcac gtcgtccagc atgatgacgt agcgcttctc acccgtgccc
     4621 ggcagcacca ggaaacgcgg cagggtgtcg gtcgagactt cgatcagcgc gtgccgcggt
     4681 ttggcgccgt tctgcttcga ctccaagcgc accgccaggt agatgacgcc gttcttgagg
     4741 tatggaaact cggggagcgt gtcgaggatg accggtacca gcgtgggtcg caccgagtcc
     4801 tcgaagtagc cctgcacgaa ttcgcggtgt tcggggagca gctggcgctc gtcgatcaag
     4861 aacacgtttt ctttttcgag cgcggcgcgc acttccgcga acacgcggtc gaagcggccg
     4921 cgctgctcga ccacgacctc atggatctgc ttcaagacct tcttggggct gcccccgtag
     4981 accatcttgt tggagtcgat tcccgcatcg accattctct ggagggtggc cacccgcacg
     5041 cggaagaact cctccaagtt cgacgagaag ataccgatga acttgagccg ctcgaccagg
     5101 ggcacggacg gatccgaggc ctcttggagc acacggtcgt tgaacgagag ccaactgatc
     5161 tcgcggttga t
//
LOCUS       DASPGK010000196         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_50955, whole genome shotgun sequence.
ACCESSION   DASPGK010000196 DASPGK010000000
VERSION     DASPGK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50955"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VEC56_05530"
     CDS             complement(<1..630)
                     /locus_tag="VEC56_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXV13649.1"
                     /translation="MSKRDYYEVLGVERAAGKDEIKKAYRKLAFQYHPDKNPGSKDAE
                     EKFKEATEAYEVLSDPEKRRVYDQYGHAGFGRGAGGQDPFAGARGFGGFEGFDISDAL
                     RAFMREFGGGGFGGGFGDFDGGGRGGPRARKGRDLQVRVSLTIAEIASGVEKQIRVTK
                     QVPCKTCHGSGAKDGSRPATCETCQGTGQIKQVQRTILGQFINVVECHAC"
     gene            complement(805..2355)
                     /gene="dnaK"
                     /locus_tag="VEC56_05535"
                     /pseudo
     CDS             complement(805..2355)
                     /gene="dnaK"
                     /locus_tag="VEC56_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
BASE COUNT          341 a          783 c          760 g          471 t
ORIGIN      
        1 gcacgcgtgg cactccacga cattaatgaa ctgccccagg atcgtgcgct ggacctgttt
       61 gatctgcccg gtgccctggc aggtctcgca cgtcgccggc cgcgagccgt ctttcgcacc
      121 cgagccgtgg cacgtcttgc acggcacctg cttggtgacg cgaatctgct tctcgacccc
      181 gctcgcgatc tcggcgatgg tcagcgacac gcgcacctgc aggtcgcgtc ccttgcgcgc
      241 gcgcgggccg ccacgacccc cgccgtcgaa gtcgccgaag ccgcccccga agcctccgcc
      301 gccgaactcg cgcatgaacg cgcgcagcgc gtcggagatg tcgaaccctt cgaagccacc
      361 gaagccgcgc gcccccgcga aggggtcttg cccgcccgcg ccgcgcccga agcccgcgtg
      421 accgtactgg tcgtacacgc gccgcttctc ggggtcgctc aagacctcgt aggcctcggt
      481 cgcctccttg aacttttcct cggcgtcctt gctgccgggg ttcttgtcgg ggtggtactg
      541 gaaggcgagc ttgcggtagg ccttcttgat ctcgtccttg cccgcggccc gttcgacgcc
      601 caggacttcg tagtaatcgc gtttgctcat cgttgtttcg agaataccgg accgcgtcaa
      661 tatatagcgg gaagcgcgga gtccggaagg ggcaaaacgc ccccaaacga acgcgcccgg
      721 gatgacccgg gcgcgtcgag ggattttacc ggcgcacggg cgccgcgttc ggtcggccta
      781 gcggaagtcg cccgcgtcgg gtgcgctgta catgagctcc gcgaagcggt gcatgatcgt
      841 ctgaagacga ccggtcgcct gcttgagcgc ggtcacgtcg cggttgtcgc ccacgcgggc
      901 tccgcgcacg tccgcgatcg cggacttgat ctcttcgagg tcgtgcgcct ggaagcgctc
      961 ggcgaagtcc ttgagcgttt gttccgtcga gtagaccagg atctcggcct ggttgacggc
     1021 ctcctggaac tcgcgcttct tcttgtcctc gtcggcgtac ttcacggcgt cttcgaccat
     1081 gcgcttgatc tcggcctccg agatgccgct cgcgaccttg atcgcgatct gctgctggcg
     1141 gccggtgccg aggtccttgg cggaaacatt gacgatgccg ttggcgtcga tgtcgaaggt
     1201 aacttcgatc tggggcacgc cgcgcggcgc gggcggaatc ccgaccaggt cgaagcgccc
     1261 cagcgtgcgg ttgtccttgg agagctcgcg ctcgccttgc agcacgtgga cgctcaccgc
     1321 gggctggttg tcggacgcgg tcgagaagat ctgcgtcttg cgcgtcggga ccgtggtgtt
     1381 gcgcgtgatg agcttggtca tcacaccgcc cagggtctcg atgccgatcg agagcggcgt
     1441 cacgtcgagc aggatcacct cttcgacctg ccccgtcagc acgccgccct ggatggccgc
     1501 gcccttggcc accacttcgt ccgggtcgac gcccttctgc ggttcctgat tgaagaattt
     1561 cttcacggcc tcgcgcaccg cgggcatgcg cgtggaaccg ccgaccagga tcactttgtc
     1621 gatctgtgtg gtcgcaatgc cggcgtcgtg gagggcggtg cgacacggct cgatggtctt
     1681 gcgaatcagc ggttcgacca gagactcgag cttcgcgcgc gtcagctgca gttcgaggtg
     1741 cttcggaccc gagtcgtcgg cggccaagaa cggcagattg atggtcgtct ccagcgtggt
     1801 cgacagctcg cacttggcct tctcggcgga gtcgcgcacg cgctgcatcg ccatcttgtc
     1861 gcccgagatg tcgatcccgc actgtttctt gaactcgtcc aggatccaat ccatgatgat
     1921 gcggtcgaag tcgtcgccgc ccaagtgggt gtcgcccgcg gtcgacttca cctggtagac
     1981 gccgtcggca agttccagga tcgagatatc gaaggtgccc ccgcccaagt cgtacacggc
     2041 cacgacaccc gtcttctgct tgtcgagccc gtacgcgagc gccgcggcgg tgggctcgtt
     2101 gatgatgcgg agcacgtcga gaccggcgat gcggcccgcg tccttggtgg cctggcgctg
     2161 cgagtcgttg aagtacgccg gaaccgtgat gaccgccttc tcgacctctt cgcccagaaa
     2221 gtcctcggcg acttttttca acgtctgcag gatcattgcg gagatttctt cggggcggaa
     2281 gttctggtcc ttgaccttga tcaggacttc gccgctgcgg ccggtctcga cggtgtacga
     2341 caccatcgtg gactc
//
LOCUS       DASPGK010000197         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_287372, whole genome shotgun sequence.
ACCESSION   DASPGK010000197 DASPGK010000000
VERSION     DASPGK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287372"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /gene="pgk"
                     /locus_tag="VEC56_05540"
     CDS             complement(<1..589)
                     /gene="pgk"
                     /locus_tag="VEC56_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXV13650.1"
                     /translation="MKKLSIRDCDVKGKRVLARVDFNVPLDSDRVITDDTRIIESLPT
                     VRDIVDRGGRLILMSHLGRPRGQKNPELSLAPVAARLAELVKNPLRFAHDCVGDEVRQ
                     RVAAMKDGDILLLENLRFHAGEEENAPAFSKELASLGDIYVNDAFGTAHRAHASTVGV
                     TEYLQTRAAGFLMEKELKALGEALNTPARPFVAVLG"
     gene            complement(602..1600)
                     /gene="gap"
                     /locus_tag="VEC56_05545"
     CDS             complement(602..1600)
                     /gene="gap"
                     /locus_tag="VEC56_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXV13651.1"
                     /translation="MAIKVAINGFGRIGRLVYRHAFNDPRFDIVAVNDLTSPTILAHL
                     LKYDSVHRRFPADVAPTETGIKVGAKTLQVISEKDPAKLPWKQLGVDVVIESTGLFTK
                     RDDAAKHIAAGARRVLISAPATDPDLTVVMGVNDKEFDAAKHAIVSVASCTTNCLAPV
                     AKVVNDNFGIVRGLMMTIHAYTNDQVMLDQPHKDLRRARAAAVSMIPTTTGAAKAVTK
                     VLPELKGKIDGTAVRVPVVDGSLVDFTVEVEKPASIEAVNKAMRDASQGAMKGIIEYT
                     EDPIVSVDVIGNAHSAIFDAKSTMAIGDRFFKLLAWYDNEYGYSGRMVDMLRLIGR"
     gene            complement(1817..>2411)
                     /locus_tag="VEC56_05550"
     CDS             complement(1817..>2411)
                     /locus_tag="VEC56_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13652.1"
                     /translation="TIGTRAGDSIELVSGGLVPGPSDTPASQATLAVWPAFETDDRIL
                     AVIHALKFSRRERLAPWLAGALAAGLPARALQGDAREIVLVPVPMDRAAQRRRGFNQA
                     ERIASALGALTGVAVERRAIAKTRATAPQSSLGRDQRVRNVAGAVSAVDARALEGRCV
                     VLVDDLVTTGATAAACAVAAWSARAALVRVVCVGYRP"
BASE COUNT          389 a          809 c          797 g          416 t
ORIGIN      
        1 cgcccagcac cgcgacgaag ggacgcgcgg gggtgttgag cgcctcgccg agtgccttga
       61 gctctttttc catgaggaaa ccggccgcgc gcgtctggag gtactcggtc accccgaccg
      121 tcgacgcgtg cgcgcggtgc gcggttccga acgcgtcgtt gacgtagata tcgcccagcg
      181 acgccagctc cttcgagaac gcgggtgcgt tctcttcctc tcccgcgtgg aagcggaggt
      241 tctcgagcag aagaatgtcg ccgtccttca tggccgccac tcgctggcgg acttcgtcgc
      301 cgacacagtc gtgggcgaag cgcagcgggt tcttgaccaa ctcggccagg cgcgccgcca
      361 ccggggccag gctcagctcg gggttcttct gcccccgcgg ccgtcccagg tggctcatca
      421 agatcaagcg accgccgcgg tccacgatgt cgcgcacggt cggcagcgac tcgatgatac
      481 gcgtgtcatc ggtgatgaca cgatcgctgt ccagaggcac gttgaaatcc acgcgcgcca
      541 gaacgcgctt gcccttgacg tcacaatcac gaatggagag ttttttcatc cgtcctcacc
      601 ctcatcgtcc gatgagacgc agcatgtcca ccatacggcc cgagtagccg tactcgttgt
      661 cgtaccaggc gagaagcttg aagaaccgat cgccgatcgc catcgtcgac ttggcgtcga
      721 agatggcgga gtgcgcgttg ccgatcacgt cgaccgacac gatcgggtcc tcggtatatt
      781 cgatgatgcc cttcatcgca ccctgcgacg cgtcgcgcat ggctttgttg acggcctcga
      841 tcgaggcggg cttttccact tcgacggtga agtccaccag cgagccgtcg accaccggga
      901 cgcgcaccgc ggttccgtcg atcttgccct tgagctccgg caacaccttg gtgacggcct
      961 tggcggcacc ggtggtggtc ggaatcatcg acaccgcggc cgcgcgcgcg cgacgcaagt
     1021 ccttgtgggg ttgatccagc atcacttggt cgttggtgta ggcgtggatc gtcatcatga
     1081 ggccgcgcac gatgccgaag ttgtcgttga ccaccttcgc caccggggcc aggcagttgg
     1141 tcgtgcagga cgcgaccgag acgatggcgt gcttggcggc gtcgaactcc ttgtcgttga
     1201 ctcccatgac caccgtgaga tccgggtcgg tggcgggcgc cgagatgagc acgcggcgcg
     1261 cgccggcggc gatgtgcttg gcggcgtcgt cgcgcttggt gaagagtccc gtcgattcga
     1321 tgacgacgtc gacgccgagc tgcttccacg gcagcttggc cggatccttc tcggagatca
     1381 cctgcagcgt cttggcgccg accttgatcc cggtctcggt gggcgcgacg tcggccggaa
     1441 agcgacgatg cacggagtcg tatttgagaa ggtgcgcgag aatggtcggg ctcgtgaggt
     1501 cgttgaccgc gacgatgtcg aagcgcgggt cattgaatgc gtgccgatag accagccgtc
     1561 cgatgcgtcc gaatccgttg atcgcaacct tgatcgccaa gtcgagtcct ccttggatgg
     1621 cgcggccttc cccgcgcctt ccgatgcttt cctcgggttc ccggtactcc gcgggccgag
     1681 gtaccaacgt aatggaaaag ctgccggagt ggcaaacgca tcgtggaacc aggatgcgcc
     1741 gggcgcggta tcgtgatggt atcccctgcc ggaactcgtt gtcaagattg aagaagcgcc
     1801 gccttcccgc caaaagctac gggcggtagc ccacgcacac cacgcgcacc agcgccgctc
     1861 gcgcgctcca cgccgccacg gcgcacgcgg ccgcggttgc tccggttgtg accaggtcgt
     1921 ccaccagaac cacgcaacga ccttccagcg cgcgcgcatc gaccgccgac accgcaccgg
     1981 ccacgttgcg cacgcgctgg tcgcgtccga gggacgactg cggtgcggtc gcgcgcgtct
     2041 tcgcgatcgc gcgccgctcc acggcgacgc cggtgagcgc gcccaacgca ctcgcgattc
     2101 gttcggcttg gttgaatccg cgccgccgct gcgccgcgcg gtccatcggc accggcacga
     2161 gcacgatctc gcgcgcgtcg ccctgcagcg cgcgcgcggg taagccggcc gcgagcgccc
     2221 ccgcaagcca cggcgcgagt cgctcgcgac gcgaaaactt caaggcgtgg atcacggcga
     2281 gaatgcggtc gtcggtctcg aaggcgggcc acaccgcgag agtggcctgc gacgcgggcg
     2341 tgtccgacgg gccggggacg agaccaccgg acaccagctc gatcgaatcg cccgcgcgcg
     2401 tgccaatggt c
//
LOCUS       DASPGK010000198         6496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_287521, whole genome shotgun sequence.
ACCESSION   DASPGK010000198 DASPGK010000000
VERSION     DASPGK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287521"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEC56_05555"
     CDS             <1..203
                     /locus_tag="VEC56_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HXV13653.1"
                     /translation="DLAVDGNDVVRETGIRPGPKVGAMLEALLERVTDDPALNTRERL
                     LDILRATGESAASGRIDRGGEE"
     gene            complement(364..960)
                     /locus_tag="VEC56_05560"
     CDS             complement(364..960)
                     /locus_tag="VEC56_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13654.1"
                     /translation="MAMMGSRSFLEFHYRPVFESVLGKEFPYKSRATAAVRSLRCNSI
                     GCATVAARRPEPGSAGGDSLAGNADSLSGWVVVLSLMTEERHEKREADAEVRLFLRGR
                     PHGVIRKMEARPSITLSNLEMCPAGIADGDTALRAGSDEGRPGRDTGGVGGCRRLVPV
                     QAAGVPRFVEDANEAPVVNWYARPASHRERITLKKQNG"
     gene            953..2872
                     /gene="dnaK"
                     /locus_tag="VEC56_05565"
     CDS             953..2872
                     /gene="dnaK"
                     /locus_tag="VEC56_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXV13655.1"
                     /translation="MAKTIGIDLGTTNSCVAIIEGGEPKVIPNPDGARTTPSIVAITK
                     DGERLVGQLAKRQAITNPQNTVFSIKRFIGRKFSEVTQESKMIPYRVAEGPDGTVLVE
                     ISGQKYSPPEISAMVLQYLKSAAEAYLGEPVTDAVITVPAYFNDSQRQATKDAGKVAG
                     LNVKRIINEPTAAALAYGLDKKKDETIAVYDLGGGTFDISILDLGEGVFEVKATNGDT
                     HLGGDDFDHRVIEWLVAEFKKEQGIDLSKDPIALQRLKEAAERAKCELSTAMQTDINL
                     PFITADATGPKHLNVKLTRAKLEQLIEELVERTVGPCKRALSDAGITAADIDEVILVG
                     GSTRIPMVQRKVKELFGKDPHMGVNPDEVVAIGAAIQAGVLGGDVRDVLLLDVTPLSL
                     GIETLGGVMTKLIDRNTTIPTRKGEVFSTAADGQTAVDIHVLQGEREMAMDNKTIGRF
                     QLAGIPPAPRGMPQIEVTFDIDADGILNVSAKDKATGKEQKIVIKASSGLSEAEIERM
                     VKDADQFRADDKRKREEVDARNAADNLVYQTEKNMKEHKDRIDAGDVIKLEAGVEAVK
                     SALKGTNGSDIKAATERLNAIWQEVAQRMYQQASAAGASPQGDGGQPAQGDGEKGPDG
                     VADAEYEVIDENKKP"
     gene            2981..3057
                     /locus_tag="VEC56_05570"
     tRNA            2981..3057
                     /locus_tag="VEC56_05570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3015..3017,aa:Val,seq:gac)
     gene            3099..5021
                     /gene="thrS"
                     /locus_tag="VEC56_05575"
     CDS             3099..5021
                     /gene="thrS"
                     /locus_tag="VEC56_05575"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXV13656.1"
                     /translation="MVRVTLPDGSTRAFDQNEVPVSDVVGLLGQRAQGDTVGVLFGGQ
                     LRDVYQSVTGEGTLRPLRDGDEQSLYLLRHTASHVLAHAVQELFPDAKFAIGPPIDEG
                     FYYDFEVEKPFTPEDLARIETRVHELLKKDLPISREVWDKARARAHFAKLGQRYKLEL
                     IDDIPGDTVSIYTVGNFVDLCAGPHIDSSRRIKYVKLLSTAGAYWRGDSKQPMLQRIY
                     ATAFFKKSDLDAYLTRLAEAEKRDHRRLGKALDLFDVKDEAGGGLVFWHPKGAIVREQ
                     MEQYLKQEYRARGYQLVFTPHIAKADLWKTSGHLTYYRDNMFMMDVEGQEYVVKPMNC
                     PGHILIYKRKLYSYRELPVRIAEMGTVYRRELSGALHGLLRVRGFTQDDGHIFCAPEQ
                     LPSEVDHSLDFALSLLQRFGFDRFAVELSVRDPANPSKYAGTDEEWTMAEAALEEAVK
                     RRDLSYKRMEGEAVFYGPKIDVKVIDAIGRPWQLSTIQFDFNLPRRFDVNYVASGGER
                     RPVFMVHRALFGSIERFMGILTEHYAGAFPTWLAPVQCVVLPVSNETLGFAERVRDAL
                     SARGVRVEMDAREEKIGYRVREAELHKVPYMAVVGEREAKEGAVAVRTRGKGMVGTKP
                     LEAFVTEIVDEVAAGR"
     gene            5245..5745
                     /gene="infC"
                     /locus_tag="VEC56_05580"
                     /pseudo
     CDS             5245..5745
                     /gene="infC"
                     /locus_tag="VEC56_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            5795..5989
                     /gene="rpmI"
                     /locus_tag="VEC56_05585"
     CDS             5795..5989
                     /gene="rpmI"
                     /locus_tag="VEC56_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXV13657.1"
                     /translation="MPKLKTSRSARKRFRKTGTGKVRRYHAFKNHILTKKTSKRKRAL
                     RKASLVHPADLKRIRRLILV"
     gene            6086..6451
                     /gene="rplT"
                     /locus_tag="VEC56_05590"
     CDS             6086..6451
                     /gene="rplT"
                     /locus_tag="VEC56_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXV13658.1"
                     /translation="MPRTKNTVAGRARRKRVLKQAEGFRGGRGKLYRTARETVDRALV
                     YAYRDRKQRKRHFRSLWIARINAAARLNGLSYSRFMNGLKKASVDIDRKMLAEMAVND
                     AAGFKKLADVAKASGSAGR"
BASE COUNT         1330 a         2051 c         2086 g         1029 t
ORIGIN      
        1 acgacctcgc ggtggatgga aacgacgtcg ttcgcgagac cgggattcgt ccggggccca
       61 aggtcggggc gatgctcgag gccctcctcg aacgcgtcac cgacgacccg gccctcaaca
      121 cgcgcgaacg gttgctcgat attctgcgag cgacgggcga atcggcggcg agtggaagga
      181 ttgatcgagg cggggaagaa tagaaaagca gaaagcccgc gtaaggggtg tcaggggtcc
      241 tttttggcag gtgctagagg acggatcctc caaccaacaa aaaggtcgag taggtactgg
      301 ggcaagaacg tccctacgct gggctttctg cgaaaatgaa tctagtccca ctcgaaaaca
      361 ttgtcaaccg ttttgtttct ttaaggttat tctttctcga tggctcgccg ggcgcgcata
      421 ccaattgacg acgggcgctt cgttggcatc ctcaacgaag cgtgggacgc ctgcggcctg
      481 gactgggacc agccgccggc accctccgac gccgccggtg tcgcggcctg ggcggccttc
      541 gtccgagcca gcgcggagcg cggtatcgcc gtcggcgatc cccgctgggc acatctcgag
      601 attcgacagc gtgatggacg gccgcgcttc catcttgcgg atgactccgt ggggtcggcc
      661 ccgaaggaag agccggactt cggcgtcggc ctcgcgcttc tcatgtcgct cctcggtcat
      721 cagcgaaagc acaacgaccc atcctgacag cgagtcagca tttccggcca ggctgtcgcc
      781 accggcagag ccgggttcgg ggcgccgtgc cgcgacggtc gcgcaaccta ttgaattgca
      841 acgtagtgaa cgaaccgccg cggtggcacg tgacttgtaa gggaattctt tgccgagcac
      901 ggattcgaat accggccggt aatggaattc aagaaaggaa cgcgatccca tcatggccaa
      961 gacaataggc atcgacctag gtactacgaa ttcgtgcgtc gcgatcatcg aaggcgggga
     1021 gcccaaggtc atccctaatc cggacggcgc tcgcaccaca ccctcgatcg tcgccatcac
     1081 caaggacggc gagcggctgg tgggccagtt ggccaagcgc caggcgatca ccaacccgca
     1141 gaacacggtg ttttccatca agcgcttcat tgggcgcaag ttttccgagg tcacgcagga
     1201 gagcaagatg atcccatacc gggtggccga aggacccgac gggaccgtcc tggtcgagat
     1261 ttcggggcag aagtactcgc cccccgagat ctcggccatg gtgctgcagt acctcaagag
     1321 cgcggccgag gcctatctgg gcgaaccggt caccgacgcc gtcatcaccg tgccggcgta
     1381 cttcaacgac agccagcgcc aggccaccaa ggacgccggc aaggtggcgg gcttgaacgt
     1441 caagcgcatc atcaacgagc cgacggccgc cgccctcgcc tacggcctcg acaagaagaa
     1501 ggacgaaacc atcgcggtct acgacctcgg cggcggcacc ttcgacatca gcattctcga
     1561 cctcggtgaa ggtgtgttcg aagtcaaggc gaccaacggc gacacccacc tgggcggtga
     1621 cgacttcgac caccgggtca tcgagtggtt ggtagccgaa ttcaagaagg agcagggcat
     1681 cgacctgtcg aaggacccga tcgcgctcca gcgcttgaag gaagctgccg agcgcgcgaa
     1741 gtgcgaactc tcgaccgcga tgcagaccga tatcaatcta ccgttcatca cggccgatgc
     1801 caccggcccg aagcacctga acgtgaagct cacgcgcgcc aagctcgagc aactcatcga
     1861 agagctggtc gagcgcaccg tgggcccgtg caagcgcgcg ctctcggacg cgggcatcac
     1921 cgccgccgac atcgacgaag tgattctggt cggcggttcg acgcgcatcc cgatggtgca
     1981 gcgcaaggtc aaggagctct tcggcaagga cccgcacatg ggtgtcaacc cggacgaagt
     2041 ggtcgccatc ggcgcggcca ttcaggccgg cgttctgggc ggcgacgtgc gcgatgtgct
     2101 tctgctcgac gtgacaccgc tctcgctcgg catcgagacg ctcggtggcg tcatgaccaa
     2161 gctcatcgat cgcaacacga ccattccgac ccgcaagggt gaagtgttct cgaccgcggc
     2221 cgacggccag accgcggtcg acatccacgt cttgcaaggc gagcgcgaga tggcgatgga
     2281 caacaagacc atcgggcgct tccagctggc ggggattccg cctgcgccgc gcggcatgcc
     2341 gcagatcgag gtgaccttcg acatcgacgc cgacggcatt ctcaacgtgt ccgccaagga
     2401 caaggcgacc ggcaaggagc agaagatcgt catcaaggcc tcgagcggtc tgagcgaagc
     2461 cgagatcgag cgcatggtga aggacgccga tcaattccgc gccgacgaca agcgcaaacg
     2521 cgaggaagtc gatgcgcgca acgccgccga caacctggtg taccagaccg agaagaacat
     2581 gaaggagcac aaggatcgca tcgacgccgg cgatgtcatc aagctcgaag cgggagtcga
     2641 ggcggtcaag tcggcgctca agggcaccaa tgggagcgac atcaaggcgg cgaccgaacg
     2701 cttgaatgcg atctggcagg aagtggcgca gcgcatgtac cagcaggcct cggctgccgg
     2761 cgcctcgcca cagggcgacg ggggccagcc ggctcaaggc gacggcgaga aaggccccga
     2821 cggagtcgcc gacgccgaat acgaagtgat cgacgagaac aagaagccat agcgcggacc
     2881 gcgcaccgag gggacgcccc cggtgcgcgc ctcgcggctt tccccttgac ccccagcgtc
     2941 tctcgacgta cgctcgcctg tctctaacgt gaaccggttt gggcgattag ctcagttggc
     3001 tagagcgtct gctcgacacg cagaaggtca gaggttcaag tcctctatcg cccaccattc
     3061 tcgtctctcc acaccgtaaa gcggagttta gaacaccgat ggtccgcgta accttgccag
     3121 acggctctac ccgagccttc gaccagaacg aagtccccgt ctccgacgtc gtcggcctcc
     3181 tcggccagcg cgcgcagggg gacacggtgg gcgtcctgtt cggcggccag ctgcgcgacg
     3241 tgtaccagag tgtcaccggc gaagggacgc tgcgcccgct gcgcgacggc gacgaacaaa
     3301 gcctgtatct cctgcgtcac accgcgtcgc acgtgctcgc ccacgccgtg caggagctct
     3361 ttcccgacgc gaaattcgcc atcggcccgc ccatcgacga gggcttctac tacgacttcg
     3421 aagtcgagaa gccgtttacg cccgaagacc tcgcgcgcat cgagacacgc gtgcacgagc
     3481 tcttgaagaa ggacctgccc atcagccgcg aggtgtggga caaggcgcgc gcgcgtgcgc
     3541 acttcgcgaa gctcggccag cgctacaaac tcgagctgat cgacgacatc cccggcgaca
     3601 cggtgtcgat ctacaccgtc ggcaacttcg tcgatttgtg cgccggcccg cacatcgatt
     3661 cgtcgcggcg catcaagtat gtgaagctgc tctcgaccgc gggcgcgtac tggcgcggcg
     3721 actccaagca gcccatgttg cagcgcatct acgcgaccgc cttcttcaag aagagcgacc
     3781 tcgacgcgta cctcacccgc ctcgcggagg ccgagaagcg cgaccaccgt cgcctgggga
     3841 aggcgctgga tctcttcgac gtcaaggacg aggcgggcgg cggcctggtg ttctggcacc
     3901 ccaagggtgc catcgtacgc gagcagatgg agcagtacct caagcaggag taccgcgcgc
     3961 gcggctacca gctcgtgttc acgccgcaca tcgcgaaggc cgacctgtgg aagacctcgg
     4021 gtcatctgac gtactaccgc gacaacatgt tcatgatgga cgtggagggc caggagtacg
     4081 tcgtaaagcc gatgaactgc ccgggccaca tcctgattta caagcgcaag ctgtacagtt
     4141 atcgcgagct cccggtacgc atcgcggaga tgggcaccgt gtaccggaga gagttgtcgg
     4201 gcgcactgca cgggttgctg cgcgtgcgcg gcttcacgca agacgacggg cacatctttt
     4261 gtgcgccgga gcagctaccg agcgaggtcg accactcgct ggatttcgcg ttgtcgctgt
     4321 tgcagcgctt cgggttcgac cgcttcgcgg tcgagctttc ggtgcgcgac ccggccaacc
     4381 cgtccaagta cgccggcacc gacgaggaat ggacgatggc ggaagcggcc ctcgaggagg
     4441 cggtcaaacg ccgggatctc tcctacaagc gtatggaggg cgaagcggtg ttctacggtc
     4501 ccaagatcga cgtcaaggtg atcgacgcca tcggccggcc gtggcagctc tcgaccatcc
     4561 agttcgactt caacctgccg cgccgtttcg acgtgaacta cgtcgcgagc gggggcgaac
     4621 ggcgcccggt attcatggtg caccgcgccc tgttcggttc gatcgaacgc ttcatgggga
     4681 tcctgaccga gcactacgcg ggcgcctttc cgacctggct cgcaccggtc cagtgcgtgg
     4741 tactcccggt ctccaacgaa acgctggggt tcgccgagcg cgtgcgcgac gccttgagcg
     4801 cgcgcggcgt gcgggtggag atggacgcac gcgaggagaa gatcggctac cgggtgcggg
     4861 aggccgagct ccacaaggtc ccgtatatgg cggtggtggg cgagcgcgag gccaaagaag
     4921 gggccgtcgc ggtgcggact cggggcaagg ggatggtcgg gaccaagccg ctcgaagcct
     4981 tcgttacgga aatcgttgac gaggtcgccg ccggccgcta atattcggcc tcgctcgcgg
     5041 gtggccttcc gggtgcccaa gggcttacca tatcgacgca agcaggggat tcacccctca
     5101 ccggccgtcc ggcagtgacg ggccaggttc gttcgacccc cccgcgagag gctcgcgggg
     5161 caagcggatg cctggcgtcc gcttattttt tttttggtgg caggaggaag ccctatcagc
     5221 ttcgaccgca agagagcgcc ccgtatccga gccaacgaag gcattcgggt gcccgagatt
     5281 cgcgttatcg ggtccgacgg aaaccaactg ggcgtgatgc cgacgcacga ggcgctgcgc
     5341 ttggcgcgcg aggaagggct cgacctcgtc gaagtatcgc cgacgtcacg gcctcccgtt
     5401 tgccggatca tggacttcgg caagtacaag tacgagcaga gcaagaaaac caaacagtcc
     5461 aagaagaagc agcacacgtt ccaggtcaag gaggtcaagt tccggcccaa gaccgaggag
     5521 cacgactatc agttcaagaa acggcacgcc gaggagttcc tcgagaagca ttacaaggtc
     5581 aaggtcacgc tgatgtttcg cggccgcgag ctcgaccaca aggaaatggg gtatcgaatg
     5641 ctcgaccgcc tcaccaagga cttggcccac gtgggcgtcg tcgagaggaa cccggagttc
     5701 gagggccgtt tgatggtgat gtatctgtcg ccgctgtcga cgaagacggc cgccaagccg
     5761 cggcccaagc cgacaccaac aaaggaaaac gaaaatgcca aagttgaaga caagccgatc
     5821 ggcgaggaaa cgctttcgta agacggggac tggcaaggtc cgccgctacc acgcgttcaa
     5881 gaaccacatc ctgaccaaga agacgtcgaa gcggaagcgc gcgctgcgca aggcgtcgct
     5941 ggtccacccg gccgatctca agcgcatccg ccggctgatc ctggtctagc aaggtttccc
     6001 gaacgattca tcgtcccaga gtgacgaggg acggcgcgcc cgcctgggca gaggggcgcc
     6061 gtagccgtat caaggaggat tcgtcatgcc acgcacgaaa aacacggtcg ccggtcgcgc
     6121 ccgacgcaag cgcgtactca agcaagcgga aggattccgc ggcggccgtg gcaagctgta
     6181 tcgtaccgcg cgcgagaccg tcgaccgcgc cttggtctac gcctaccgcg atcgtaaaca
     6241 acggaagcgc cacttccgct cgctgtggat cgcgcgcatc aacgcggccg cgcgcttgaa
     6301 cggcctgtcc tacagtcgtt tcatgaatgg gttgaaaaag gcgtcggtcg atatcgaccg
     6361 caagatgctc gcggagatgg ccgtcaacga cgccgccgga ttcaagaagc tcgccgacgt
     6421 cgccaaggcg tcgggcagcg cgggccgcta gtcgtcgtcg acccgatgga ccctcgctcc
     6481 atcgagaact cgctgc
//
LOCUS       DASPGK010000199         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1691429, whole genome shotgun sequence.
ACCESSION   DASPGK010000199 DASPGK010000000
VERSION     DASPGK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691429"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VEC56_05595"
     CDS             <1..1107
                     /locus_tag="VEC56_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXV13659.1"
                     /translation="DTLVITTPARVAGEAATPSRALAIETRAFATETAARTPSPCARA
                     ADAVARARPDPALAARVRTRDVLAGWWVTSAPEARYPAQPGFSMSASKLGSYTRCARQ
                     FFYREILDIEEDESIYLQVGSLVHDALKEIIAPGATGDEVLAALQHAGTREIAERLVT
                     DTFKDAGAWMRELSVKYLEDMLRYVAQLESQRQGSYRVRVVEETVEAVIEGMPLRGRF
                     DRVDDVDGVGPVIIDYKTSGKGKIRKTYPSLVENLDTDYWQIPVYGAVSEVNDLHAAA
                     FVFYALPPGEEGFAVGVQLKPGTHPAPIPTGKRPHVRYGPVDTVAIADAMAYAVEIHR
                     AIVEGETRYERTDDIGICPNCYFARICQRSKASL"
     gene            1080..>2103
                     /locus_tag="VEC56_05600"
     CDS             1080..>2103
                     /locus_tag="VEC56_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV13660.1"
                     /translation="MSEKQSIALDHGQRAAVEAPVAAATNIIAGAGTGKTTVLVERYL
                     ALLETGIPIERLLALTFTLKAAGEMRERVRVEVARRHASLARRVSWILNFHQFGYRFI
                     QENAPALGIDPGVDVVTIAEFQRIQRYLRTRFENGRVPGVPPDFGGEPPPPTKLGSLF
                     DTLMKVVHHCRGIMMDPAALRAMVREDDHPAYIARVDTVIALAREYEQELARRGLLDF
                     SDMITIPARALMQDGPLARAYANAFDHILVDEFQDTSAAQNELLRALSGGDFSRVTVV
                     GDRKQSIYRWRDARVENIREFPDPAPRELKLNYRSRQPILDLAHALVVREDELKDAAV
                     ELEAHRG"
BASE COUNT          389 a          743 c          645 g          326 t
ORIGIN      
        1 gataccctcg tgatcaccac gcccgcgcgc gtcgccggcg aggccgcgac accgtcgcgc
       61 gcgttggcca tcgaaacgcg cgcgtttgcc accgaaaccg ccgcccgcac cccatcccca
      121 tgcgcacgcg ccgcggacgc cgtcgcccgc gcgcgacccg acccagcgct cgccgcgcgc
      181 gtacgcacgc gggacgttct cgccggctgg tgggtgacga gcgcgccgga ggcgcgctat
      241 ccggcgcagc cgggattctc catgagcgcg tcgaagctcg gttcgtacac ccgttgcgcg
      301 cgtcagttct tctatcgcga gatcctcgac atcgaagagg acgagtccat ctatctccag
      361 gtcggaagcc tcgttcacga cgccttgaag gagatcatcg cgcccggtgc gaccggcgac
      421 gaggtgctgg ccgcgctcca acacgccggc acgcgcgaga tcgccgagcg cctcgtcacc
      481 gacaccttca aggacgccgg cgcgtggatg cgcgagctgt ccgtgaagta cctcgaagac
      541 atgctgcgct acgtcgccca actggaatca cagcgccagg gaagctatcg cgtgcgcgtg
      601 gtggaagaaa cggtcgaagc ggtgatcgaa ggcatgccgc tgcgcggtcg cttcgatcgt
      661 gtggacgacg tggacggcgt gggtcccgtg atcatcgact acaagacgtc gggcaagggc
      721 aagatccgga aaacgtaccc gtcgctggtc gagaacctcg acaccgatta ctggcagatc
      781 cctgtgtacg gcgcggtatc agaggtcaat gacctccacg ccgcagcatt cgtgttctac
      841 gcgctcccgc ccggcgagga gggtttcgcc gtgggcgtgc agctcaagcc gggcacccat
      901 ccggcaccga ttccgaccgg aaagcggccc cacgttcgct acgggcccgt cgataccgtg
      961 gccatcgccg acgccatggc atacgccgtc gagatccacc gcgccatcgt ggagggagag
     1021 acccgttacg aacgcaccga cgacatcggc atctgcccca actgttactt cgcgaggata
     1081 tgtcagagaa gcaaagcatc gctctagacc acggccaacg agccgccgtc gaagcccccg
     1141 tcgccgccgc gaccaacatc atcgcgggcg cggggacggg gaagaccacg gtgctggtgg
     1201 agcgctactt ggcgcttctc gagaccggca taccaatcga gcgccttctc gcgttgactt
     1261 tcactctgaa ggccgcagga gagatgaggg agagagtccg cgtcgaagtg gcaaggcgac
     1321 acgcgtcgct ggcgcgacgc gtatcctgga tcctcaactt ccaccaattc ggctatcgct
     1381 tcattcaaga gaacgccccc gcgctcggca tcgatcccgg cgtggacgtc gtcacgatcg
     1441 ccgagttcca gcgcatccag cgctacctgc gcacgcgttt cgaaaacgga cgcgtccccg
     1501 gtgtcccgcc cgacttcggg ggcgagcctc cgccgcccac caagctcgga agcctcttcg
     1561 acacgctcat gaaagtcgtc caccactgcc gcggcatcat gatggacccc gcggcactac
     1621 gtgcgatggt gcgcgaagac gaccaccccg cctacatcgc gcgtgtcgac accgtcattg
     1681 ctcttgctcg tgaatacgag caagagctcg ctcgccgcgg gctcctggac ttcagcgata
     1741 tgatcacgat cccggcgcgc gcgctgatgc aagacggccc gctggcgcgg gcctacgcca
     1801 acgcgtttga ccatatcctc gtcgacgagt tccaggacac cagcgccgcg cagaacgaac
     1861 tcttgcgcgc cctctccggc ggcgacttct cgcgcgtcac cgtggtgggg gaccgcaagc
     1921 agtctattta tagatggcgc gacgcgcgcg tggagaacat ccgcgagttc cccgaccccg
     1981 cgccgcgcga gctgaaactc aattaccgct cgcgccaacc catcttggac ctcgcacacg
     2041 cgcttgttgt acgcgaagac gaactcaagg acgccgccgt ggaactcgaa gcccaccgcg
     2101 gcc
//
LOCUS       DASPGK010000200         4662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1691580, whole genome shotgun sequence.
ACCESSION   DASPGK010000200 DASPGK010000000
VERSION     DASPGK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691580"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VEC56_05605"
     CDS             complement(<1..238)
                     /locus_tag="VEC56_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXV13661.1"
                     /translation="MHSLDAIFKPRSIAVIGATPRPGSIGREILNNLFANEFNGKIFP
                     VNPKYEYIHSTKAYPSVTDIPDPVDLAVIVVPAAL"
     gene            complement(246..689)
                     /locus_tag="VEC56_05610"
     CDS             complement(246..689)
                     /locus_tag="VEC56_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXV13662.1"
                     /translation="MTQPPKKTKVLFVCIGNMCRSPMAEGFARKYGADFLDVYSAGTN
                     ATGVVSEDSIELMRELKIDIAKAMSNGLEAVPVAEMDVVVSMAPVRARALVPPGFRGK
                     TIDWKVEDPVGKSLTVFRRVRDQLRGLVKQLVDDLRRERERDRVA"
     gene            complement(717..1454)
                     /locus_tag="VEC56_05615"
     CDS             complement(717..1454)
                     /locus_tag="VEC56_05615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HXV13663.1"
                     /translation="MSARGPQRDGVDDLVRDKSRTPAMFDAIARRYDLLNHLLSFNID
                     RRWRRELVNFAGVREGERVLDVATGTADVAIEFAKRTGAGAIVGLDPSAGMIAVGREK
                     LARASLADRVTLVDGDALALPFDDASFDVVTIAFGLRNLPDYRRGVAEMTRVLRTGGR
                     LLVLDFAPPRGARFMVQRAYFSTLLPFAGKAVSGYGGAYRYLVASIRDFMDEGGVRDL
                     FGSHGLAGFEARHMTGGIVMLYRGVKS"
     gene            complement(1451..2587)
                     /locus_tag="VEC56_05620"
     CDS             complement(1451..2587)
                     /locus_tag="VEC56_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXV13664.1"
                     /translation="MSAIASGAGAQDHPRYRAVEAFPSLEFRRPIDLQSPRDGSNRLF
                     VAEQDGVVRVFTNRADTRASAVFLDIRDKVNTDGNEMGLLGLAFSPSFATDKSFFVDY
                     TASKWTRRITRVARFRADATGSTAVPASEEVVIEIAQPWSNHNGGQVAFGPDGMLYIA
                     MGDGGSAGDPQGNGQNRAALLGKILRLDVSKRPYAIPADNPFRGNPDGWKEEVWAYGL
                     RNPWRFSFDRETGQLWTGDVGQVTWEEINVIEQGKNYGWDCREGDGPYEPSHERSSAC
                     ADAGNFVDPVWSYGRTEGISVTGGYVYRGSALPDLVGWYVYADYGTGRVWALRVENGE
                     PENRLLVDTDLMISSFGVDEADELYICAHDYRGEPTKIFKLVAE"
     gene            complement(2617..3798)
                     /locus_tag="VEC56_05625"
     CDS             complement(2617..3798)
                     /locus_tag="VEC56_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXV13665.1"
                     /translation="MAILSCAALVWVACDDDDPQAPTQDGPPLTDPIWEYGRSQGQSI
                     TGGYVYRGPSVAALTGKYVYADHGSRRIWALTYDGVTATNEFLADMPDGLSSFGVAED
                     GELFACGYNSVNATKLRGFSESGGVYTLTDAFPNLTFTKPVDLQNAGDGTDRLFVVEQ
                     GGIIRVFPNDPATTSTTEFLNITVQCCGEMGLLGLAFHPDYENNGYFFVYYSLAGGTP
                     WRARLSRFQVGADPNDADESTEKILLEFNDDFGNHNGGALCFDDDGYLYVAIGDEGDG
                     GDPNQNAQNRAVLFGKILRLDVDQNVDIVPYHGIPADNPYAGNTWGFREEVFAWGLRN
                     PWRMSYDAATDRIWVGDVGQGSYEEIDIIESGKNYGWDCREGMHDYLGPPDGPSPACP
                     Q"
     gene            complement(3918..4601)
                     /locus_tag="VEC56_05630"
     CDS             complement(3918..4601)
                     /locus_tag="VEC56_05630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HXV13666.1"
                     /translation="MNRVHCIEHVPFETPGRIADWVVERRHSFASTRVYDREPFPALD
                     SFDALVVMGGPMGVHDDERFGWLADEKRLIDAALRADKRVLGVCLGAQLIAHVLGARV
                     YRNRFQEIGWFPVQIAEAGRAPWGLPKEMLAFHWHGDAFDLPKDAVHLAKSAACEHQM
                     FALGTNVLGIQFHLEVTRAGVEDMLRHGAADLGEGPYVQTPAAMLAPEPAFMASHALL
                     DAILDHWSA"
BASE COUNT          801 a         1556 c         1513 g          792 t
ORIGIN      
        1 ccagcgcggc cgggaccacg atcaccgcca ggtccaccgg gtcggggatg tccgtcaccg
       61 acgggtacgc cttggtgctg tggatgtact cgtacttggg gttgacgggg aaaatcttcc
      121 cgttgaactc gttggcgaac aggttgttga ggatctcgcg tccgatgctg ccggggcgcg
      181 gggtggcccc gatcacggcg atcgagcgcg gcttgaagat ggcgtcgagg gagtgcatag
      241 cgatgctatg cgacgcggtc gcgctcgcgt tcgcgacgaa ggtcatcgac cagctgcttc
      301 acgagtccgc gcagctgatc gcgcactctc cgaaataccg tcagcgactt gcccaccggg
      361 tcttccactt tccaatcgat ggtcttgccg cggaagccgg gcggtaccaa cgcgcgagcg
      421 cgcacggggg ccatagaaac caccacgtcc atttcggcga cggggaccgc ctcaagtccg
      481 ttggacatcg ctttcgcgat gtctatcttg agctcgcgca tgagctcgat ggagtcctcg
      541 ctgaccaccc cggtcgcgtt ggtgccggcg ctatagacgt ccaggaagtc cgcgccgtac
      601 ttgcgcgcga atccctcggc catgggcgag cggcacatgt tgccgatgca gacgaagagc
      661 accttggttt tcttgggtgg ttgggtcatc ggaacgcggc gaacagcgtc acgctatcac
      721 gacttgacgc cgcgatacaa catcacgatg ccgccggtca tgtggcgggc ctcgaaaccg
      781 gcgagcccgt gggagccgaa cagatcgcga acgccgccct cgtccatgaa atcgcggatg
      841 gaagccacca ggtagcggta ggcgccgccg tagccggaca cggccttgcc cgcgaacggg
      901 agcagggtcg agaaatacgc gcgctggacc atgaagcgcg cgccgcgcgg gggtgcgaag
      961 tcgagcacca gcaggcgccc gccggtgcgc agcacgcgcg tcatctcggc cacgccgcgg
     1021 cgatagtcgg ggagatttcg gaggccgaag gcaatcgtca ccacgtcgaa ggacgcgtcg
     1081 tcgaaaggaa gcgccagcgc gtcgccgtcc accagcgtca cgcggtccgc gagcgacgcg
     1141 cgcgcgagct tctcgcgccc caccgcgatc atccctgccg acgggtcgag ccccacgatg
     1201 gcgcccgcac cggtccgctt cgcgaactcg atcgcgacgt cggcggttcc ggtggccacg
     1261 tcgaggacgc gctcgccctc gcgcacgccc gcgaagttca ccagctcgcg ccgccaacgc
     1321 cggtcgatgt tgaagctcaa gaggtggttc aggaggtcgt agcggcgcgc gatggcgtcg
     1381 aacatcgcgg gggtgcggct cttgtcccgc accaggtcgt cgacgccgtc gcgctgcgga
     1441 ccgcgcgcgc tcactcggcc accagcttga agatcttcgt cggctcgccg cggtaatcgt
     1501 gcgcgcagat gtagagctcg tccgcctcgt cgacgccgaa ggacgagatc atgaggtcgg
     1561 tgtctaccag caggcgattc tcgggctcgc cgttctccac tcgcagcgcc cacacccggc
     1621 cggtgccgta gtcggcgtag acgtaccacc ccacgaggtc cgggagcgcg ctgccgcggt
     1681 acacgtagcc acccgtaaca ctaataccct cggtgcgccc ataggaccac accgggtcga
     1741 cgaagttgcc cgcgtcggcg cacgcggacg agcgctcgtg agacggctca tagggcccat
     1801 cgccctcgcg gcaatcccaa ccgtagttct tcccctgctc gatcacgttg atttcttccc
     1861 acgtcacctg tccgacgtcg ccggtccaca actgaccggt ctcgcgatcg aacgagaatc
     1921 gccacgggtt gcgaagcccg taggcccaga cttcttcctt ccaaccgtcc gggttgccgc
     1981 gaaacgggtt gtcggcggga attgcgtacg gccgcttgga aacgtcgagg cgaaggatct
     2041 tgccgagcag tgctgcgcgg ttctgcccgt tgccctgcgg gtcgcctgca ctcccgccgt
     2101 cgcccatcgc gatgtagagc atgccgtcgg ggccgaaggc cacttggccg ccgttgtgat
     2161 tcgaccacgg ctgcgcgatc tcgatcacga cttcctcgga ggcggggacc gccgtgctcc
     2221 cggttgcgtc ggcgcgaaag cgcgccacgc gcgtgatgcg gcgcgtccac ttggacgcgg
     2281 tgtaatcgac gaagaaggat ttgtcggtcg cgaacgaggg cgagaatgcg aggccgagca
     2341 ggcccatctc gttcccgtcg gtgttgactt tgtctcggat atccaggaac accgcgctcg
     2401 cgcgcgtatc ggcgcgattg gtgaacacgc gcacgacgcc gtcctgttcg gcgacgaaca
     2461 agcggttgct cccgtcgcgc gggctctgga ggtcgatcgg gcgccggaac tcgagcgacg
     2521 ggaacgcttc caccgcgcga tagcgcggat gatcttgcgc gccggcgccg gacgcgatcg
     2581 ccgacacggc aacgactacg gcgaattgcg cgcgcatcat tgcgggcagg ccggcgacgg
     2641 gccgtccggc ggtccgaggt agtcgtgcat gccttcgcgg cagtcccaac cgtagttctt
     2701 cccgctctcg atgatgtcga tctcttcgta gctcccctgc ccgacgtcac ccacccagat
     2761 gcggtcggtg gcggcgtcgt acgacatgcg ccacggattg cgcaggcccc acgcgaaaac
     2821 ctcctcgcga aaaccccacg tgttgccggc gtacggattg tcggcgggga taccgtgata
     2881 cgggacgatg tcgacgttct ggtcgacgtc gagacggagg atcttcccga aaaggaccgc
     2941 gcgattctgc gcgttctggt ttggatcgcc gccgtcgccc tcgtcgccga tggccacata
     3001 gaggtagccg tcgtcgtcga agcacaaagc gccgccgttg tggttgccga agtcgtcgtt
     3061 gaactcaagc aagatcttct cggtcgactc gtccgcgtcg ttggggtcag caccgacttg
     3121 gaagcgcgag aggcgcgcgc gccagggcgt gccgcccgca agggagtagt agacgaagaa
     3181 gtagccgttg ttctcgtagt cggggtggaa cgcgagtccg aggagtccca tttcaccgca
     3241 gcattgcacc gtaatattca ggaactccgt cgtgctcgtc gtggcggggt cgttgggaaa
     3301 cacacgaatg atccctccct gctccaccac gaaaagacga tcggttccgt cacccgcgtt
     3361 ctggagatcg acgggtttgg tgaaggtcag gttcgggaac gcgtcggtca gcgtgtagac
     3421 gccccccgac tcgctgaagc cgcgcagctt ggtcgcgttc accgaattgt acccgcacgc
     3481 gaacagttcg ccgtcttccg cgacgccgaa cgacgagagc ccgtccggca tgtcggccag
     3541 aaactcgttg gtcgcggtaa ccccgtcgta ggtgagcgcc catatgcgcc gtgagccgtg
     3601 gtcggcgtag acgtacttgc ccgtcagtgc cgcgacggac gggccgcgat acacatagcc
     3661 gcccgtgatc gattgcccct gactacgccc gtactcccag atcggatcgg tgaggggcgg
     3721 cccgtcctgg gtgggtgcct gcgggtcgtc gtcgtcgcac gcgacccaga ccagcgcggc
     3781 gcaggatagg atggccacga cacgcctcca cgaggtgctt cgggcgcggg ttgtcgaatc
     3841 gtgtggcatg ggaacctccg aaccggggcg gcctgacgcg agcgtagccc actcgcgctg
     3901 cgaagtaaac cagacgatca cgcgctccag tgatccagga tcgcgtcgag gagcgcgtgc
     3961 gaagccatga acgccggctc cggcgccaac atcgcggccg gcgtctggac gtacggaccc
     4021 tctcccaagt ccgcggcacc gtggcgcagc atgtcttcca cacccgcacg cgttacttcc
     4081 aagtgaaact ggattccgag cacgttcgtc ccgagcgcga acatctggtg ctcgcacgcc
     4141 gcactcttcg cgaggtggac agcgtccttg ggcaaatcga acgcgtcgcc gtgccagtga
     4201 aacgcgagca tctccttggg gagcccccac ggcgcgcgac cggcttccgc aatctgcacc
     4261 ggaaaccagc cgatctcctg gaagcgattc cggtacaccc gcgcgcccag cacgtgcgcg
     4321 atcaactggg cgccgaggca tacgccgagc acgcgcttgt ccgctcgcag cgccgcatcg
     4381 atcaaacgct tctcgtccgc gagccagcca aagcgctcgt cgtcgtgcac gcccatgggc
     4441 ccgcccatga cgaccagcgc atcgaaggaa tccagcgcag gaaacggctc gcggtcgtat
     4501 acccgcgtcg acgcgaacga atgcctgcgc tcgaccaccc agtcggcgat gcgccccggc
     4561 gtttcgaacg gcacgtgctc gatgcaatga acgcggttca tttcggctgt tcgttcaacg
     4621 tccaatcgta ctcgaagtat ttcacgacgt acttcccttc tt
//
LOCUS       DASPGK010000201         8812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_288035, whole genome shotgun sequence.
ACCESSION   DASPGK010000201 DASPGK010000000
VERSION     DASPGK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288035"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VEC56_05635"
     CDS             complement(<1..642)
                     /locus_tag="VEC56_05635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV13667.1"
                     /translation="MDLRSGTLLLHYRLVEKLGEGGMGVVWRAVDTTLDREVAVKILP
                     DVFASDADRAARFEREAKVLASLNHPNITTIHSVHKADSVRFLVMELAHGEDLATHLA
                     RGALPRDEAVHIAKQIAEALEAAHESGVVHRDLKPANITVTRDGKVELLDFGLAKALD
                     TAASGVASSSSDLSKSPTQVSGQTAAGMILGTAAYMSPEQARGKPVDRRADIWA"
     gene            complement(652..1173)
                     /locus_tag="VEC56_05640"
     CDS             complement(652..1173)
                     /locus_tag="VEC56_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002194916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HXV13668.1"
                     /translation="MDDLRYPIGQFDFKQAVTMDRVRGAIDEIAACPAALRSAIRGFD
                     DAKLDTPYRDGGWTVRQVVHHVPDSHMNAYIRLKLGLTEDNPTIKPYEQQLWAQLPDA
                     REPIGPSLANLDAVHERWVSLLRQLEEKDFSRTIFHPENGSMTLGLLTLHYAWHGKHH
                     VAHLTSLRKRMGW"
     gene            1255..2529
                     /locus_tag="VEC56_05645"
     CDS             1255..2529
                     /locus_tag="VEC56_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV13669.1"
                     /translation="MVYARADGSILDHPGLLLAGVGGGPVRPVRDDELIPLPRGSDLF
                     LLPGRRPIGLEPASGAVVEWDEDGATAVAAFLAPAWTMFLHPAYRTLPDAPALPLFAY
                     STVGFADGQFWATGVRVDQDVRQDPWRFDRDRLAKQVAKGVAQSPDNLVVQQLQTCAL
                     TYGCRAAQNFFIGRHEAPIPTSVACNSKCIGCISLQPDGEFRASHDRIRRAPRPQEIA
                     DLAVAHIERVPDGVVSFGQGCEGEPLLGRELLPEAVRQIRARTSHGTVNVNTNGSLPE
                     VVDAMCRAGLDAIRVSMNSARDEVYTAYYRPKKYGFDDVRETLRVMRRHDRYRSINYL
                     VFPGVTDTDEELDAISSFIEDADLDLIQMRNLNIDPELYRERLPVGAVREGFGIGVFM
                     GRLKDRFPHLHYGYFNPSKETYARWRSQRAAC"
     gene            2596..6180
                     /locus_tag="VEC56_05650"
     CDS             2596..6180
                     /locus_tag="VEC56_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13670.1"
                     /translation="MLKRSCLIVVCLALLTPTVAPAADEHLAGLTLIVVDAPDIAALH
                     RARALVQENGGRVGVMVPPSILIGWVDPATANTLAGREGIRDIRWSEADPARYHASDE
                     QSLSAIESFNAVVSGAYARRKRAEIVEQAAASATQPRVSDAFPREASNPSDVLDNLRA
                     AGFDEPTLAERGLLPRDAQDGSASLTYADKMAGTVAVTFFLVESDGTGSDPDSWTWTP
                     EDMQEYMDDTVVGLLWWSNRADDHLDCWVTFLINHVSAFDPRCHQWVEPSLHDNGFVT
                     TWAANVMTKMGYVSGNHFSKVDAFNAWQEATYQANSAYSAFIAYNPIGAPSSFANGGY
                     AFAWIYGPYFWSLFRTPGWTHDVVVSHETGHIFGACDEYTGGCSSCNSTCSAYGGNNE
                     NCEDCNPLSRPCMMRSNENGLCQYTKTMIGWDASSPCAPPPPAPLPTPTLALVSPDDG
                     LVGTQVTLTLTGSNFVAGVQVDLGADVFVHATTLTNSTTLQVWASVFNDAAPGPVDVV
                     VRNRDGQTAVLAGAFELLPTRRHYFSPSGGNVFPFITPGDAGVALADVMAAASDGDTV
                     LVPTMTFSGFSLSIAKGITLQGAWNASFTARNLATGKTAIDLDGIVDFYPSSDGAGID
                     GFLLENGDGRADFFPAVARYAGSVRVFQSTIAIRNCEIRNGTTGPSDQLGYGGAIYGY
                     QSSVTIENCHVHGNSATKGGAVYLEQCSGSISGSAFEYNAVLTTGLNQPEGSCVYIAA
                     SSNVALTDNTIDHHSGGQNGGGILVENSTDVVVEGGVIEYNSASFGGGGIAFKATSGT
                     LRRVVVRRNTGLFAGGVMLTGAGARDVAECRIEWNSAMFGGGLSADGTLSLTHNLFVG
                     NSATNLSGALGISNVASGVVAGNTFDRNSVGSGAGGINLSSVTVPVFNNIVTNTTGTG
                     IACAGAAPSLFDFNNVWNASGGSYSGCTPGAGSISADPLFVDTTLVDYHLGLHSPCID
                     RGRTGASFNDPDGSRGDMGWYGSHAFTMDQPAFVENASVGWSGTDRILNWDGSPEADV
                     AQYAVYADAASGFVPGAANLVAIVAAPATSYLVGPIASDTYFKVNAIDDDGYAGGYSV
                     EASAQPSTGSGPTSYRTRLFQNHPNPFNPETEIRFELAVPSNVSITVFDAAGRLVRVL
                     ENGNRPAGEHTVRWNGRNDAGGPVSSGVYFYRMSATGFDQTRKMVLLK"
     gene            6286..7521
                     /locus_tag="VEC56_05655"
     CDS             6286..7521
                     /locus_tag="VEC56_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV13671.1"
                     /translation="MDAAQRRILATLLFSVFATVIGVGIVVPLLPIHAHGLGASGFQI
                     ALIFGAYAMSRALFLPVFGHWSDRRGRKRFLTVGLFAYAIISIALAYLDSIAALVVIR
                     LLHGVASAMLIPVFQAYAADLAPAGREGRVMGVYGTVVLVGMSLGPFLGGLVNDHWGL
                     HATFWIMGLLALTGFAVCAAMLPPASSEPVLRIAKQPHAWLDLLRDRGLAGLFAFRFA
                     YVVCVGIIWAFVPLYASFKLSLSSASIGLIITLGIASGGTLNMPMGLLADRASKKLLV
                     VAGGLMAACGILGFQWADSQRAFILASVCFGAGGGICMPALMALAAQKGSRSDAMASV
                     MALMTVAHSLGMLAGALLGGFMMDLLALRWVFTAGAVVMVLGVAHFLSFAIERRAERR
                     PERTSATAELAIDAVGVRQ"
     gene            7532..8578
                     /locus_tag="VEC56_05660"
     CDS             7532..8578
                     /locus_tag="VEC56_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXV13672.1"
                     /translation="MVPGWLLALVVAAAAASAPTPSPRAIPFRTQTMGTWATVTLVTA
                     DSTAVADLAYRSLLTLHRVDSLMSNWTQTSEVARINREAGAREVVVHPDVASVLALAQ
                     GVTRATDGAMDITVEPVVRLWGFLGGTRRVPTQAEITKALASVGPDKLRFDPARAAVR
                     FTNPDTKIDLGGIAKGYGVDRVVSILRAAGATNALVDLSGNMVALGDAPGKKGWSVGI
                     RDPSGRRPHLGTIRLHDEAISTSGSYEQFVDAAGKRYGHIIDPRTGWPAEGIVSVTVV
                     SATATLCDAWDTGLFVLGSEKARGLARTHDEFSIVVVEPRPDGGYVVWVEESLRSRFE
                     IESGSVSTITLRYF"
BASE COUNT         1384 a         3039 c         2867 g         1522 t
ORIGIN      
        1 ggcccagatg tcggcgcgcc ggtcgacggg cttgccgcgc gcctgctcgg gcgacatata
       61 ggccgccgtg cccaggatca tccccgccgc ggtctgcccc gagacctggg tgggcgattt
      121 cgacagatcg gacgacgacg acgcgacccc ggacgccgcg gtgtcgagtg ccttggccaa
      181 cccgaagtcg agcagctcca ccttgccgtc gcgcgtcacc gtaatgttgg cgggcttcaa
      241 gtcgcggtgg accacgccgc tctcgtgcgc cgcttccagc gcctccgcga tctgtttcgc
      301 gatgtggacg gcctcatcgc gcggcagcgc gccccgggcg aggtgcgtcg cgaggtcctc
      361 accgtgcgcc aactccatca ccaggaagcg cacgctgtcg gccttgtgca ccgagtggat
      421 ggtggtgatg ttgggatgat tgagcgacgc gagcaccttc gcttcgcgct cgaagcgcgc
      481 ggcacggtcg gcatcgctcg cgaagacgtc ggggagaatc ttgaccgcga cttcgcggtc
      541 gagggtggtg tcgaccgcgc gccacaccac acccatgccg ccctcgccga gcttctcgac
      601 gaggcgatag tggaggagca gggtgccgga gcgcaggtcc aagtcgtccg actaccagcc
      661 catgcgcttg cgcagcgagg tgaggtgggc gacgtggtgc ttgccgtgcc acgcgtagtg
      721 cagtgtcagg agtcccagcg tcatggagcc gttctcgggg tggaagatgg tgcgcgagaa
      781 atctttctct tcgagctggc gcaagagcga cacccagcgc tcgtgaacgg cgtcgaggtt
      841 tgcgagcgag ggaccaatgg gctcgcgcgc gtcggggagt tgggcccaca gctgctgttc
      901 gtagggcttg atggtggggt tgtcctcggt gagcccgagc ttcaatcgga tgtacgcgtt
      961 catatggctg tcgggaacgt ggtgcacgac ctggcgcacg gtccaaccgc cgtcgcggta
     1021 gggcgtgtcg agcttggcgt cgtcgaagcc gcgtatcgcg gagcgcaggg ccgccgggca
     1081 cgcggcgatc tcgtcgatcg cgccgcgcac gcggtccatc gtgaccgcct gtttgaagtc
     1141 gaattgaccg atgggatagc gcaggtcgtc catggcgtcg cagcatacca cggccgcgcg
     1201 tcggtgtgat agagtcggtg catgcctctc gccaaaccga aggaaccggt gcgcatggtc
     1261 tacgcgcgag ccgacgggtc gatcctcgac cacccgggcc tgcttctggc cggtgtcggc
     1321 ggaggacccg tgcgccccgt gcgcgacgac gagttgatcc cgctcccgcg cgggagcgac
     1381 ctatttctgc tcccgggacg acggcccatc gggctcgaac cggcgagcgg tgcggtggtc
     1441 gagtgggacg aagacggcgc aaccgcggtc gcggcgttcc tggcccccgc gtggaccatg
     1501 ttcctccatc ccgcgtaccg aaccctcccc gacgcacccg cgctcccgct cttcgcgtac
     1561 tcgacggtcg ggttcgccga cgggcagttt tgggcgacgg gcgtgcgcgt cgatcaagac
     1621 gtccgccagg atccgtggcg cttcgaccgg gaccgacttg cgaagcaagt cgccaaaggc
     1681 gtcgcgcagt ctcccgacaa tctcgtggtt cagcagctcc agacgtgcgc cctcacctac
     1741 gggtgccgcg cagcgcagaa cttcttcatc gggcgccacg aagcgccgat tcccaccagt
     1801 gtcgcgtgca attcgaagtg catcgggtgc atctcgctgc aacccgacgg cgagtttcgc
     1861 gccagccacg accgcattcg ccgcgcacca cgcccccaag agatcgccga cctggcggtc
     1921 gcgcacatcg agcgcgttcc cgacggcgtg gtgagcttcg gccagggatg cgagggcgag
     1981 ccgctcctcg gccgcgagct cctgccggaa gcggtgcgac agattcgcgc ccgcacctcg
     2041 cacgggacgg tcaacgtcaa taccaacgga agcctgcccg aagtggtcga cgccatgtgc
     2101 cgcgccggcc tcgacgcgat tcgggtttcg atgaacagcg cgcgcgacga ggtctacacc
     2161 gcgtactacc gccccaagaa gtacggcttc gacgacgtcc gcgaaacgct gcgcgtcatg
     2221 cggcgccacg accggtatcg gtccatcaat tacctggtgt tcccgggggt taccgatacc
     2281 gacgaggagc tggacgcgat ctcctccttt atagaggacg ccgacctgga cctgatccag
     2341 atgcgtaacc tcaacatcga cccggagctg taccgcgaac ggctgccggt tggcgccgtg
     2401 cgcgagggct tcgggatcgg ggtgttcatg gggcgcctga aggatcgctt tccgcacctg
     2461 cactacggct acttcaatcc atccaaggag acgtacgcgc gctggcgatc gcagcgcgcg
     2521 gcgtgctaga atcgagggcc gagggcttcc ctcggcgctc gcctgagcga cgtcaccatc
     2581 ggaggccttc tgctcatgct caagcgctcg tgcctgatcg ttgtctgcct cgccctgctc
     2641 accccgaccg tcgcccccgc cgccgacgag cacctcgccg gcctgacact catcgtcgtc
     2701 gatgcgcccg acatcgcggc gctgcatcgc gcgcgcgcat tggtgcaaga gaacggcggg
     2761 cgggtgggcg tgatggtgcc cccgtcgatc ctgatcggat gggtcgatcc ggccaccgcg
     2821 aacacgctcg ccggccgcga aggcattcgc gacattcgct ggtcggaagc ggaccccgcg
     2881 cgttaccacg ccagcgacga gcagtcgctg tccgccatcg agtccttcaa cgccgtcgtg
     2941 agcggagcgt acgcgcgccg caagcgcgcg gaaatcgtcg agcaagccgc tgcgtcggca
     3001 acgcagccgc gcgtctcgga tgcattcccg cgcgaggcgt cgaacccctc cgacgtgctc
     3061 gacaatcttc gcgccgccgg cttcgacgag cccacgctcg cggagcgcgg gctgctcccg
     3121 cgtgacgccc aagacggctc ggcgtcgttg acgtacgccg acaagatggc gggcaccgtc
     3181 gcggtgacgt tcttcctggt cgagagcgac ggcaccggca gcgacccgga ttcgtggacg
     3241 tggacgcccg aggatatgca ggagtacatg gacgacaccg tggtcgggtt gttgtggtgg
     3301 agcaaccgtg ccgacgacca cctcgactgc tgggtgacct ttctgatcaa ccacgtgtcc
     3361 gccttcgatc cgcgctgcca ccagtgggtc gagccctcgc tgcacgacaa cggcttcgtc
     3421 accacctggg ccgccaacgt gatgacgaag atgggctacg tctccggtaa ccacttcagc
     3481 aaagtggacg cgttcaacgc gtggcaggag gcgacgtacc aggccaacag cgcctactcg
     3541 gccttcatcg cctacaaccc catcggtgct ccctcgtcct tcgccaacgg aggctacgcg
     3601 ttcgcgtgga tctacggccc gtatttttgg agcctgtttc ggactcccgg ctggacccac
     3661 gacgtcgtgg tgtcgcacga gaccgggcac atcttcggcg cgtgcgacga gtacaccggc
     3721 ggttgctcgt cgtgcaactc gacgtgcagc gcgtacggcg gcaacaacga gaactgcgag
     3781 gactgcaacc cgctctcgcg gccgtgcatg atgcgctcca acgagaacgg cctgtgccag
     3841 tacaccaaga ccatgatcgg gtgggacgca tccagcccgt gcgcgccccc gccgccggca
     3901 ccgctgccga cccccaccct cgcgctggtg tcccccgacg acggactcgt cggcacccag
     3961 gtcacgctga ccctcacggg ctcgaacttc gtggccggcg tccaggtcga cctcggcgcg
     4021 gacgtgttcg tgcacgcgac cacgctcacc aactcgacca cgctgcaggt gtgggcgtcc
     4081 gtgttcaatg acgccgcgcc gggtccggtc gacgtcgtgg tgcgaaaccg cgacggccag
     4141 accgccgtcc tcgcgggcgc gttcgagctg ctgcccacgc ggcgtcacta cttctcgcct
     4201 tcgggcggca acgtgtttcc gttcatcacg cccggcgacg ccggggtcgc gctcgccgac
     4261 gtcatggcgg ccgccagcga cggcgacacc gtgctcgtcc ccaccatgac cttctcggga
     4321 ttctcgcttt cgatcgccaa gggcatcacg ctccagggcg catggaatgc gtccttcacg
     4381 gctcgcaacc tcgctaccgg caagaccgcg atcgatctgg acggcatcgt cgacttctat
     4441 cccagctccg acggcgcggg catcgacggc ttcctgctcg aaaacggcga cgggcgcgcg
     4501 gacttcttcc ccgccgtcgc ccgctacgcg gggtcggtgc gcgtgttcca gagcacgatc
     4561 gcgattcgca actgcgagat ccgtaacggc accaccgggc cgtcggacca gctcggttac
     4621 ggcggcgcga tctacggcta ccaatcgagc gtgacgatcg agaactgcca cgtgcacggc
     4681 aatagcgcca ccaagggagg agccgtttac ttggagcagt gttcgggctc gatttccggc
     4741 tcggccttcg agtacaacgc ggtactgacg accgggctca atcagcccga gggttcgtgc
     4801 gtgtacatcg cggcctcgag caacgtcgcg ctcaccgaca acaccatcga ccatcacagc
     4861 gggggccaga acggcggcgg catcctggtc gagaactcga ccgacgtagt ggtcgaaggc
     4921 ggcgtgatcg aatacaacag cgcgagtttc ggaggcggcg gcattgcgtt caaagccacc
     4981 agcggtaccc tccggcgcgt cgtcgtgcgc cgcaacacgg gcctcttcgc gggaggcgtc
     5041 atgctcaccg gtgcgggagc gcgcgacgtc gcggagtgcc gcatcgagtg gaattcggcc
     5101 atgttcggcg gcgggctctc cgcggatggg acgctttcgc tcacccacaa cctgttcgtc
     5161 ggcaactcgg cgacgaatct gtcgggtgcg ctcggaatct ccaacgtggc gagcggtgtc
     5221 gtggccggca acaccttcga ccgcaactcg gtcggttccg gcgcgggcgg catcaacctc
     5281 tcgagcgtaa ccgtccccgt attcaacaac atcgtcacca acacgaccgg cacgggtatc
     5341 gcgtgcgccg gggccgcacc ctcgctgttc gatttcaata atgtctggaa cgcgagcggc
     5401 gggtcataca gtgggtgtac accgggggcc gggtcgatct cggccgaccc gctgttcgtc
     5461 gacaccaccc tggtcgacta ccacctcggc ctgcactcgc cgtgcatcga ccgcgggcgc
     5521 accggcgcgt ccttcaacga ccccgacgga tcgcgcggcg acatgggctg gtacggttca
     5581 cacgccttca ccatggacca acccgccttc gtggagaacg cgagtgtcgg ttggtcggga
     5641 accgatcgca ttctcaattg ggatgggagt cccgaagcgg acgtcgcgca gtacgcggtg
     5701 tacgcggacg ccgcgagcgg tttcgtcccc ggcgcggcga atctggtcgc catcgtggcc
     5761 gcacccgcta cctcgtacct cgtcggcccg attgcgagcg acacctactt caaggtgaac
     5821 gcgatcgacg acgacggcta cgcgggcggt tattcggtgg aagcgagcgc gcagccgtcg
     5881 accgggtcgg gtccgacgtc gtatcgcacg cgcttgttcc agaaccaccc caatccgttc
     5941 aacccggaga ccgagattcg cttcgagctg gcggttccgt cgaatgtttc catcaccgtg
     6001 ttcgacgccg cgggtcggct cgtgcgggtg ctggaaaacg gaaatcggcc ggccggcgag
     6061 cacaccgtgc gctggaacgg ccgaaacgac gccggcgggc cggtctcgtc gggcgtctac
     6121 ttctatcgaa tgtcggcgac cggtttcgac cagactcgca agatggtgct cctcaagtag
     6181 ctcgcccgtt acccgcccag cggcagaaaa atcccgccct ccccgggaaa gtggcgctag
     6241 ttttgcctct ttttccggtc cacgaccatt accggctcga ttccgatgga tgccgcgcag
     6301 cgcagaatcc tggccactct ccttttctcc gtgttcgcca cggtaatcgg ggtgggcatc
     6361 gtcgtgccgc tcctccccat ccacgcgcac ggactgggcg cgagcggctt ccagatcgca
     6421 ctcatcttcg gcgcgtacgc gatgtcgcgc gcgctgttcc tccccgtctt cggtcactgg
     6481 tcggaccggc ggggtcgcaa gcgcttcctc accgtcggtc tcttcgcgta cgcgatcatc
     6541 tccatcgcac tcgcgtatct cgacagcatc gcggcgctgg tcgtcattcg gttgctgcac
     6601 ggggtcgcct ccgcgatgtt gatccccgtc ttccaagcct acgccgccga tctcgcgccc
     6661 gccgggcgcg agggacgcgt catgggcgtc tacggaacgg tggtcctggt cggcatgagc
     6721 ctggggccgt tcctcggcgg cctggtcaac gatcactggg gtctgcacgc gaccttctgg
     6781 atcatgggac tgctggcact gaccggcttc gcggtgtgcg cggccatgct gccgccggcc
     6841 tccagcgaac ccgtgctccg catcgcgaag cagccccacg cgtggctcga cctcctgcgc
     6901 gatcgcggac tcgcgggttt gttcgcgttt cgcttcgcat acgtggtgtg cgtcggcatc
     6961 atctgggcct tcgttcccct ctacgcctcc ttcaagttgt cgctctcgag cgcgtcgatc
     7021 gggctcatca tcacgctcgg catcgcgagc ggcggaacgc tcaacatgcc gatggggctc
     7081 ctcgccgacc gcgcgagcaa gaagctgctc gttgtcgcgg gcggactaat ggccgcgtgc
     7141 ggcatcctcg gttttcagtg ggccgacagc cagcgcgcgt tcattctggc ctcggtctgt
     7201 ttcggtgccg gcggcggcat ctgcatgccc gcgctgatgg cgctcgcggc gcagaagggc
     7261 agccgctccg acgccatggc gagtgtcatg gcgctgatga cggtcgcgca cagcctcggc
     7321 atgctggcgg gcgcactctt gggcggcttc atgatggatc tcctcgcact gcgctgggtg
     7381 ttcaccgcgg gcgcggtggt catggtgttg ggagtcgcac attttctttc gttcgcaatc
     7441 gaacgccgcg ccgagagacg ccccgagcga acgtccgcaa cggcggagct ggcgattgac
     7501 gccgtcggcg tgcgccaata gacttcgcgc catggtcccc ggttggttgc tcgcactcgt
     7561 cgtggctgcc gcggcggcgt ccgctcctac cccgtcaccg cgcgcgattc cctttcgcac
     7621 ccagaccatg ggcacgtggg ccacggtcac gctcgtgacc gccgattcga ccgctgtggc
     7681 cgacctcgcg taccgaagcc tgctcacgct ccaccgcgtc gactccctca tgagcaactg
     7741 gacacagacc agcgaggtcg cgcgcatcaa tcgtgaggcg ggcgcgcgcg aggttgtcgt
     7801 gcatcccgac gtcgcgagcg tcctcgccct cgcgcaaggc gtgacgcgcg ccaccgacgg
     7861 tgccatggac atcaccgtcg agccggtggt gcgcctgtgg ggattcttgg gcgggacgcg
     7921 gcgcgttccc acgcaagccg agatcacgaa ggcgctcgcg agtgtcggcc ccgacaaact
     7981 ccgcttcgac cccgcacgcg cagccgttcg cttcacgaat cccgatacga agatcgactt
     8041 gggcggcatc gcgaagggct acggcgtcga ccgcgtggtt tcgattctgc gcgcggccgg
     8101 cgcgaccaat gccctcgtcg atctgtcggg caacatggtg gcactcggcg acgcacccgg
     8161 caaaaaaggc tggagcgtgg gcatccgcga cccgtcgggc cgccgcccgc acctcggcac
     8221 cattcgcctc cacgacgaag cgatatcgac ctccggcagc tacgagcaat tcgtcgacgc
     8281 cgccggaaag cgctacggtc acatcatcga tccgcgcacc ggatggccgg cggaagggat
     8341 cgtgtccgtc acggtcgtct ccgctacggc cacgctgtgc gacgcgtggg acaccggcct
     8401 cttcgtcctc ggatccgaga aggcccgcgg gctcgcgagg acccacgacg agttctcgat
     8461 cgtggtcgtc gaaccacgcc ccgacggcgg ttacgtcgtc tgggtggaag agtcgcttcg
     8521 ttcccgcttc gagatcgaat cgggaagtgt gagcaccatt acgctgcgct acttctagtt
     8581 cccggctcac cacggccaat ccctcccacc tcatctcgct ggcgtagacc ttgcacgatg
     8641 gtagaccgga tctacccgtc gcaggctgac cgcgtcggca tgcggagaac caaggcatct
     8701 cgggaccgtc gaacggccga atagacgccg ggttttcccc taccgaaccg caaattgcat
     8761 tgcgatggac cctcgattgg gcgggaaggc gcgccgggcg gccaaacttg gc
//
LOCUS       DASPGK010000202         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_520838, whole genome shotgun sequence.
ACCESSION   DASPGK010000202 DASPGK010000000
VERSION     DASPGK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520838"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..562
                     /locus_tag="VEC56_05665"
     CDS             350..562
                     /locus_tag="VEC56_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HXV13673.1"
                     /translation="MTESVAREMGKAVLHVDLSTMSMGDAVAAIGGWLDGLQGETLNV
                     AGPRASSDPAIYEKAREVVARLLRGW"
     gene            661..1191
                     /locus_tag="VEC56_05670"
     CDS             661..1191
                     /locus_tag="VEC56_05670"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HXV13674.1"
                     /translation="MPRRSSKGSSSLVRTVEQSILFVRGEKVILDYVLAEMYGVTTGA
                     LVQAVRRNAGRFPIDFAYQLSPQEVTRLKSQIVISNGRGGRRRRPYAFTEQGVAMLSS
                     VLRSERAVRVNVEIMRAFVHLRRLLVSHADLARKLEELEKKYDSQFRVVFDAIRELMA
                     PDPIPEKRRIGFRRDD"
     gene            complement(1225..1788)
                     /locus_tag="VEC56_05675"
     CDS             complement(1225..1788)
                     /locus_tag="VEC56_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13675.1"
                     /translation="MRFHTKRRIATAAWVIAAVSTAFNARATEVVDQENLPGATLVYS
                     NCGIGPGSISQGFTPTMSPLIAVEIMMPPSPPAGALTVRIRQGGADGPIVGETTAPNE
                     AGWNRYDFAGGVVVLPEQLYVIEVVDLFHPAAIVLNNADTYAGGNAYGCTDNPVLIRD
                     VVFRTYAATTLPTREATWGKVKALYRE"
     gene            1974..>3262
                     /locus_tag="VEC56_05680"
     CDS             1974..>3262
                     /locus_tag="VEC56_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXV13676.1"
                     /translation="MVVIALLFVLIFILPALVTFAAEWLWFQALGYERVIGMRIVVGT
                     LLGVGVGAVAFALLDANLRLALRGAVIGPRLVQLTAGSPIELTQLVRRLARPGVIAIA
                     VLLGLSASGTWLDVLRFLRQVPFGTTDPVFGRDVGYYVFTVPLVADVIRLLTVLTTFG
                     LVASALLYVLRGEVSVRRGHVTVERAARVHLAVLVATLLVLTALRVHFVRIPGLLYST
                     TGPLVGASYADLHGTLMGLRLAALAAVVGAGLVLWGARGPRLAHATLIAVGLYIGVSL
                     LGVTIYPSIVHRLVVSPNELSKETPQLIRHIAATREAWGLDSVVTRELTGEAGLTEED
                     IRANSATIDNVRLWDRDPLLQTFGQLQEIRTYYDFRSVDDDRYWIDGRYRQVMLSPRE
                     LNAASLPTRTFINERLTFTHGMGIALGPVNQVTSEGLP"
BASE COUNT          524 a         1022 c         1070 g          646 t
ORIGIN      
        1 ggtcgccaac cggctcgcga acaccgacgc gaataccacc acactcgcaa gtacccacag
       61 cctcattccg acgagccgtg ccatctcccg tcgccccggt tctcgatagt actgcgttgc
      121 cccacgaact gtggtcgtgc acatcgctct caccctttcg tcgcggtgtt tacctcgccg
      181 ccttgtctcg gcaggcacac tcgctggccg ttgccagctg gagtgtaaat cgtcccccag
      241 cgggttaacg agtgtattgc ggcgcgcgtt ccggcgcaag tccctcaact cgaggcggga
      301 cgctttgcta gtatgacctc taggagacgc gctcgccgga ggctcgaagt tgacggagtc
      361 ggtggcccgc gagatgggga aggcggtctt gcacgtggac ctgtcgacga tgtcgatggg
      421 ggacgcggtg gcggcgattg gagggtggct cgacggcctc caaggagaga cgctcaacgt
      481 cgcggggccg cgggcgagca gcgatccggc gatttatgag aaggcgaggg aggtggtggc
      541 gcgtttgctt cgtggctggt aggaccgttg aaaccggcaa cctgatccgc taccgtttct
      601 cttcctccca aggcattcgt ctcctcccga agcaacgtcc gttccgagag gagacttcgc
      661 atgcccagac gttcttccaa gggttcgagt tcgcttgtcc gtacggttga gcagtcgatc
      721 ctgttcgttc gaggcgagaa ggtgatactg gattatgtgc tcgccgagat gtacggggtg
      781 accaccggtg ccctcgtcca agcggttcgg cgaaacgcgg gtcggttccc tatcgacttt
      841 gcgtatcagc tttcgcccca ggaggttaca cgtttgaaat cacaaattgt gatatcaaac
      901 ggacggggag gtcggcgtcg gcggccttac gcgttcaccg aacagggcgt cgcgatgctg
      961 tcgagcgtac tccgcagcga gcgtgcagta cgagtcaacg tggagatcat gcgcgcgttt
     1021 gtgcatctgc gaaggctgct cgtctcgcac gccgatctag cccgcaagct cgaggagttg
     1081 gagaagaagt atgactcgca gtttcgagtc gtcttcgacg cgatccgtga gctgatggct
     1141 cccgatccga tcccggagaa acgccgcatc ggttttcgac gggacgacta ggaattattc
     1201 ccgacgatca ttgcgtcggt ccctctactc ccggtaaagc gcctttactt tcccccacgt
     1261 cgcctcacgc gtgggtagcg tcgttgccgc gtaggtccga aagacgacgt cccgtatcaa
     1321 gaccgggttg tccgtgcagc cgtaggcgtt gccgcccgca taggtgtccg cattgttcaa
     1381 aacgatggcc gccggatgga agagatcgac tacttcgatc acgtacagct gctcgggcaa
     1441 gacgaccacg ccaccggcga agtcgtaccg gttccacccg gcctcgttcg gcgcggtggt
     1501 ctcgccgacg atggggccgt cggcgccacc ctgccggatg cgaacggtca gcgcgcccgc
     1561 cggcggcgaa ggcggcatca tgatctcgac cgcaatcaag ggcgacatgg tcggggtgaa
     1621 tccctgcgag atgcttccgg gtccgatacc gcagttcgag taaacgaggg tcgcgccggg
     1681 gagattctcc tgatcgacga cttcggtcgc gcgggcgttg aaggcagtcg agaccgctgc
     1741 gatgacccac gcggcggtcg cgattcggcg cttagtatga aatctcatct tcaaacctcc
     1801 gggttcggcg gtgggtgatc caccgcttgt ccataacgca ggaactagag acgaaagtga
     1861 tcacagattg ggacgtgaag cagaacgagc ggtattccgc catttgtggt aacgtgcgag
     1921 gcatgaccgc tcgcccccga gtgttgcgcc ctggacgccc gcggaatcgc cgcgtggtcg
     1981 tgatcgcgct gctcttcgtc ctcatcttca tcctgcccgc gctcgtcacg tttgccgccg
     2041 agtggctctg gttccaggcc ctgggatacg agcgcgtgat cgggatgcgg atcgtcgtgg
     2101 gcacgctgct cggtgtcggc gtgggggcgg tcgccttcgc gctcctggac gcgaacctgc
     2161 gcctcgcgct gcgcggggcc gtcattggtc ctcgcctggt ccagttgacc gccggcagcc
     2221 ccatcgagct gacccaactg gtgcggcggc tggcgcgccc gggggtaatt gcgatcgcgg
     2281 tgctgctggg cctttcggcc tccggcacct ggctcgacgt gctgcgcttc ctccgccagg
     2341 ttccgttcgg cacgaccgac ccggtcttcg ggcgcgacgt gggctactac gtgttcacgg
     2401 tgccgctggt cgcggatgtg atccgactgc tcacggtcct cacgactttc ggattggtgg
     2461 cgagcgcact cctgtacgtg ctgcgcgggg aagtcagcgt tcgccgcgga cacgtgacgg
     2521 tcgagcgcgc ggcgcgcgtg cacctcgcgg tgctggtcgc gacactgctg gtcctgacgg
     2581 cgttgcgcgt gcacttcgtg cgcattccgg ggctcctgta ttccaccacc ggcccgctcg
     2641 tgggcgcgag ctacgccgat ttgcacggca ctttgatggg tctgcggctg gcggcgctgg
     2701 ccgcggtggt gggggcggga ctggtgctgt ggggcgctcg cggtccgcgc ctcgcgcacg
     2761 cgactctgat cgcggtgggg ctctacatcg gcgtgtcgtt gctcggcgtc acgatctatc
     2821 cgtcgatcgt tcatcgactc gtcgtatcgc ccaatgaact gtcgaaagaa acgccgcagc
     2881 tgattcgtca catcgccgcc acgcgcgaag cctggggcct ggacagtgtc gtcacccgcg
     2941 agctcacggg cgaagcgggc ctcaccgaag aggacattcg cgccaactcg gccacgatcg
     3001 ataacgtgcg cctgtgggac cgcgatcctt tgctgcagac gttcggacag ctgcaggaga
     3061 tccgaaccta ctacgatttt cgctccgtcg acgacgaccg ctactggatc gacgggcgct
     3121 accgccaagt gatgctctcg ccgcgcgagc tgaacgcggc ctcgctcccc acgcgcacgt
     3181 ttatcaacga gagactcacg ttcacgcacg ggatggggat cgcactgggc ccggtgaacc
     3241 aggtgacgtc ggagggcttg cc
//
LOCUS       DASPGK010000203         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1222994, whole genome shotgun sequence.
ACCESSION   DASPGK010000203 DASPGK010000000
VERSION     DASPGK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222994"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2264
                     /locus_tag="VEC56_05685"
     CDS             <1..2264
                     /locus_tag="VEC56_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13677.1"
                     /translation="RLYVGNLSFSTNDGCSAQMGGASGGLIGFMDEAASFPVGGELDL
                     VVRGPAGIDPCVRVARGAAGVLVDPDFSSMGASSYRAEIWNDGAPVATFDGIGGDVNA
                     TGFPASFVADLEGVKAPLPDPFPLPAAAAFPISNGFHLRWSTDVTFSIAGGPIATGDE
                     LRMVPEGGTGLPYVSSIDVLVTIPPTLPPYTVAIDDIAQQQFGRMHRGTGGATIVSVF
                     APPIVADRRTTLSHHRGMDVSVGPSAAGGLQVDMHPSQSRDDLIWDLKDGFVTGDQDV
                     LSITAELLTGGQGQVGRVTSVYTGSGWSVTPDFSGSGSPDARVEVFDASGNLMGINPC
                     IIGPFTVASIYWPIETGVEDNETLALVLGYASPVAVDLPGVGIVTGTTIKMHSNATKR
                     HRFFAIIDRTNLLATGGEARVSAEVNGFGLPSYVGEAEFAVQGTAYAKRCHGGLVLHD
                     IDASGTDGVDIEPCLLPAGIPPDQFGIEWAPLGPPSASQGQTDWAFLSQRAMDPGPVQ
                     RMAISMGSNGTVVSLEANPGPVQTPEFRVLLKNGGQVVADFMHSDPGVAFAELRDWPI
                     GVGYQELATPDEPWSMWIDLGYEMDVLIQGGGPVNRTGAMLPLTKADLIEVIATNTPN
                     PGPSQRKILSGKFAGIGNIVLLDRGPTPTRIDTAPRSTRTVLRPAYPNPFNPSTTLAF
                     DLAQSGRVSLKIYAVDGSYVATVHEGSLRAGAHAFQWNGVDHRGQPVASGVYLADLRA
                     PDGQLRTKINLLK"
     gene            complement(2295..>3389)
                     /locus_tag="VEC56_05690"
     CDS             complement(2295..>3389)
                     /locus_tag="VEC56_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13678.1"
                     /translation="DGSGAFPWLQWSTVCSLGGSRESPLVVKSNEDFVVAWRDGRPGD
                     IFAQRIDDATGYWGIVVPPAITSVTDSPGDGGGNVDIAWNACDRDNLTTDVREYHVFR
                     SLDQSSWTQVATVDATGLASYALSNVPTTADNLTHYFYIDAVGGGLLQHHFPSQTGSG
                     VSIDDPPNIPLSLNVEKIGSWYYLNWVPPSPCVGGAVCDVVGYRVYRRVMGGPAALSP
                     TDDWTLIGESQNPQFTDQTVSPGEPQEWAVSALDAGGREGAMTAAFRIEDTTSDVGDV
                     PARFVVAPNVPNPFGHSTELVFALPDERDVRVDVYDVAGRLVHSRSLGRQPSGWNRVQ
                     LPSGSLASGVYFYRVTAGRDVSTRKMVVRR"
BASE COUNT          572 a         1135 c         1126 g          556 t
ORIGIN      
        1 accgtctcta cgtcggcaac ctctcgtttt cgaccaacga cggctgtagc gcgcaaatgg
       61 gcggtgcaag cggcgggctg atcggattca tggacgaagc cgcgagcttt ccggtcggcg
      121 gcgagttgga cttggtcgtg cgcggacctg cgggcatcga tccgtgcgtc cgcgtcgcgc
      181 gcggcgcagc cggcgtcctg gtcgatccgg atttcagctc gatgggtgcg tcgagctacc
      241 gcgcggagat ctggaacgat ggcgcgccgg tcgcgacctt cgacgggatc ggcggagacg
      301 tgaatgccac aggcttcccc gcgtcgttcg tggccgacct cgagggcgtc aaggcgccgc
      361 tgcccgatcc gtttccgctg cccgccgcgg cggcgtttcc gatttcgaac ggcttccacc
      421 tgcgatggag cacggacgtg acattctcga tcgccggcgg tccgatcgcc accggcgacg
      481 agttgcgcat ggtccccgag ggcggaaccg ggctccccta cgtctcgagc atcgacgtgc
      541 tcgtgaccat tccgcccaca ctgcccccct acaccgtggc gatcgacgac atcgcgcagc
      601 agcaattcgg gcgcatgcac cgcggcacgg gcggagcgac gatcgtcagc gtattcgcgc
      661 ctccgatcgt cgccgaccgt cgcacgacgc tgtcccacca ccgcgggatg gacgtatcgg
      721 tcggcccgtc cgcggcgggc ggactccaag tcgacatgca tccctcgcaa tcgcgcgacg
      781 acctcatctg ggacctcaag gatggcttcg tcaccggcga tcaggacgtg ctctccatca
      841 ctgccgagtt gttgaccggc ggccagggcc aagtcgggag agtgacgtcg gtttacacgg
      901 gttccggttg gagcgtgacg cccgacttca gtggatcggg ctcacccgac gcgcgcgtgg
      961 aagtcttcga cgcgtccggg aatctgatgg ggatcaatcc ctgcatcatc ggcccgttca
     1021 cggtcgcgtc gatctattgg ccgatcgaga ccggagtgga agacaacgag accctcgccc
     1081 tcgtgctcgg gtacgcgtcg ccggtcgcgg tcgatctgcc gggtgtggga atcgtgaccg
     1141 gaaccacgat caagatgcac tcgaacgcga cgaagcggca ccggttcttc gccatcatcg
     1201 atcggacgaa tctgctggca accggcggcg aggcgcgcgt cagcgcggag gtcaacggtt
     1261 tcgggctgcc gtcgtatgtg ggcgaagcgg agttcgcggt gcagggcacc gcgtacgcga
     1321 agcgatgcca cggcgggctg gttctccacg atatcgacgc gagcggcacc gacggcgtcg
     1381 acatcgagcc ctgcctcctt cccgcgggga taccgcccga tcaattcgga atcgagtggg
     1441 cgccgctggg accgccgagc gcgtcccaag gacagaccga ctgggccttc ttgagccagc
     1501 gagcgatgga tccggggccg gtccagcgca tggcgatctc gatgggctcg aacggaacgg
     1561 tcgtgagctt ggaagcaaac cccggcccgg tccagacgcc cgagtttcgc gtgcttctca
     1621 agaacggcgg gcaggtggtg gcggacttca tgcattccga cccgggcgtg gcgttcgcgg
     1681 agcttcgcga ctggccgatc ggcgtcggct accaagagct cgcgactccc gacgagccgt
     1741 ggtcgatgtg gatcgacttg ggctacgaga tggacgtgtt gattcaaggc ggcgggccgg
     1801 taaatcgtac cggcgcgatg ctaccgctca ccaaggccga cttgatcgaa gtgatcgcga
     1861 cgaacacgcc caatcccggg ccctcccaga ggaagattct ctccgggaag ttcgcgggca
     1921 tcgggaacat cgtgttgctc gaccgcgggc cgacgccgac gcgcatcgac accgcaccgc
     1981 gctcgacgcg caccgtgctg cggcccgcct atcccaatcc gttcaacccg agcaccacgc
     2041 tcgcgttcga cttggcgcag agcgggcgcg tctcgctcaa gatctacgcg gtcgacggga
     2101 gctacgtcgc gaccgtgcac gaaggatcgc tgcgcgccgg cgcgcacgca ttccaatgga
     2161 acggcgtcga ccaccgcggg cagccggtgg cgtcgggcgt gtacctggcc gatttgcgcg
     2221 cgccggatgg ccagctgcgc acgaaaatca atctactgaa gtgaatcgcg attgtaggga
     2281 cacccgatcg cgcctcaccg ccgcaccacc attttgcgcg tcgagacgtc gcggccggcg
     2341 gtgacgcggt agaagtacac gccgctcgcg agcgaaccgc tcggcagttg cacgcggttc
     2401 cagcccgacg gctgtcgccc cagcgaccgc gagtgcacca atcgaccggc gacgtcatac
     2461 acgtcgacgc gcacgtcgcg ctcgtcgggg agcgcgaaca cgagctcggt gctgtggccg
     2521 aacgggttgg gaacgttggg tgccaccacg aagcgcgcgg gtacgtcgcc gacgtcgctc
     2581 gtcgtgtcct cgattcggaa cgcagccgtc atcgcgccct cgcgtccccc ggcgtcgagc
     2641 gcggagacgg cccactcctg gggctcaccg ggcgacacgg tctgatcggt gaactgcggg
     2701 ttctgcgatt ctccgatcag cgtccaatcg tccgtcggtg aaagcgctgc cggcccaccc
     2761 atcacccgcc ggtatacccg gtagcccacc acatcgcaca cggccccgcc cacgcacgga
     2821 cttggtggca cccagttgag gtagtaccaa ctcccgatct tttcgacgtt caacgagagc
     2881 ggaatgttgg gcgggtcgtc gatcgacaca ccgcttcccg tctgcgacgg gaaatgatgc
     2941 tgcaagagcc cgccgccgac cgcgtcgata tagaagtagt gagtaaggtt gtccgcggtg
     3001 gtgggaacgt tcgagagcgc gtatgacgcg agcccggtgg catcgacggt ggccacctgc
     3061 gtccacgacg attgatcgag cgagcgaaac acgtggtact cgcgcacgtc ggtggtcaag
     3121 ttgtctcggt cgcacgcatt ccacgcgatg tccacgtttc cgccgccgtc gccgggcgaa
     3181 tccgtcaccg acgtgatcgc cggcggcact acgatgcccc agtagccggt cgcgtcgtcg
     3241 atccgctgcg cgaagatgtc gcccggccgc ccgtcgcgcc acgcgacgac gaagtcttcg
     3301 ttcgacttca cgaccaaggg ggactcgcgg gatccgccga gagagcagac cgtactccac
     3361 tggagccaag ggaacgcacc gctaccgtc
//
LOCUS       DASPGK010000204         7967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_872719, whole genome shotgun sequence.
ACCESSION   DASPGK010000204 DASPGK010000000
VERSION     DASPGK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872719"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1939
                     /locus_tag="VEC56_05695"
     CDS             296..1939
                     /locus_tag="VEC56_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV13679.1"
                     /translation="MDERTLYSQIFDAVRDPLMVVDAAGDVHAANASAIRFLDVAQDA
                     GLFGPSEDDGRDTGSPVTASVLDLVRRRLTVRSHPLYASDGRDLDIVVDVEPLPGSAA
                     LLHFRSPTERLARELWSDDAVAAVAHEIKNPLSAMRSALDVVLKDPSAPLLDSQRRML
                     GAFDRSARRLARFVDGVLNVSRLHAGAAPAPRALVSVRALVEGVVDEYRALHPHKCPR
                     LECEVAEAGARAFVDQDGAEIVLLNLLGNAARFSPSDAAVTVRASRAGVEAMEDDMRL
                     VPWDVVGKPRLARIDVEDRGLGMTPDVLAHLFDRYHASDEGELSLATARDSGTHLGLH
                     ISRAIAEAHDGWLKVESSLGEGTVASVFVPEDEATARTLSRLRIAADSARRWQRAGRE
                     LAVVALAKASPESWEDLAVRWAEAPRVDPLASTCASRKALLWPIDDHLGVAIVPLAGG
                     NDGPSVVLGEPVIRADEFAWGMSGFVAGWCRVEDATSFAQAFHKAASRMGRACAQIAR
                     GGVAEEAATPANAEAFEEAWEPVGMLPAMETTLHVENEE"
     gene            1942..2340
                     /locus_tag="VEC56_05700"
     CDS             1942..2340
                     /locus_tag="VEC56_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV13680.1"
                     /translation="MDSLRILLVDDEQDIVDTVKYSLEMRGFVVDVAYDGATALTKAR
                     TGRYAIMILDVMLPAKNGYEVSRILKDEMTRGELAPFKVLLITARKLDNAMREEFVST
                     WSKADACLYKPFDLEGLIDKISGLLDPVAR"
     gene            2579..4087
                     /locus_tag="VEC56_05705"
     CDS             2579..4087
                     /locus_tag="VEC56_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HXV13681.1"
                     /translation="MKPIQLPRSLNNPITIVGAGIALVTGVTLAFFLVLIATVGESNP
                     YIGIFVYLVLPPIFGLGVILIPVGALRKRRRVRKQLDTAALRWPVVDLNQRTYRNALF
                     FFVLGLGMLTLISVVGGYQAYHYTESVEFCGTTCHTVMEPEHTAYANSPHARVSCAEC
                     HVGAGAGWYAKSKLSGAYQVYAAAFNKYPRPIPTPIKNLRPAQETCEQCHWPEKFFGA
                     QQRQYNHYMYDEANTHWPINLLIKTGGGDPKTGQTAGIHWHMNIGVQVEYVARDERRQ
                     DIPWVRVTDRTTGRVTVYQDQVSPLSAEELAASTVRKMDCIDCHNRPSHKFNSPDNAI
                     DRAILTGAIDPSLPEIKRVAVEAMQAPYETKDDAFRAIATKIPDFYLQNYPDILAEKR
                     VAIDEAVLATQHQFSQNIFPEMKVRWEGYPDNIGHFIFPGCMRCHDGNKVSDDGWTIT
                     RDCTACHAILRQGAGENMQMAVTEEGLEFSHPDGGEDWRDTNCHDCHTGTQP"
     gene            4059..6881
                     /locus_tag="VEC56_05710"
     CDS             4059..6881
                     /locus_tag="VEC56_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV13682.1"
                     /translation="MIATREPNPDPAHCPDDRLRRAARVLALLYVTVLVAVTPAGAAN
                     PANDTCFDCHADRGDPEVPFVDGGVFGKSIHGGLACNECHADVDPDDLPHAEDLKPVY
                     CGGCHGDQQLDFDASIHGQALNRKQPYAPSCKDCHGIHDIFAPSDERSTAFKMKVPFL
                     CGQCHREGAPVANTYQISEHNILENYSESIHGEGLFKKGLMVTAACADCHGAHLILPH
                     TEPRASISPRNITATCMKCHTRIEDVHTKVIRGELWEKAPGAIPACTDCHVPHKVRRE
                     AVTVTISDRDCMKCHERHDVFKIAGADTTSMLVDKDLLDSSAHTNIPCVKCHSDVDPR
                     RHRPCEPSGLVDCSNCHAKIADEYAVSGHGQAHAKGVAEAPYCTDCHGGHGALPHLDE
                     SSPTYRASVPALCGDCHRAEGAAGAVEGLSQQDALADYSHSVHGRGLTEKGLLPSAVC
                     IDCHSSHMVLKHTDERSSVSHHNLPATCATCHRGIYKDFIKSVHFASGDQDPGKLPTC
                     ATCHSSHTIGEVEGDAFVQEVTHQCGSCHHELAETYLETMHGKAYRLGYLDAAKCSDC
                     HGAHSILDVDDPSSAVGSHNIVGTCQQCHEDANQRFTGYLTHATHHDPDKYPFLYYSY
                     WAMTFLLIGVFGFFGLHTLLWLPRSFRHMLEHRKEVAWGESRYYIRRFTLPQRVTHLF
                     VIVSFLSLALTGMMLKFSSMPWAAFIADMLGGVKGAGIIHRCAAVMTFGYFAFHVASL
                     VRFKRHRRLKLTQLVFGKDSLMFNRKDLSDFVGTMKWFFNAGPRPRYGRWTYWEKFDY
                     LAVFWGVAIIGLSGLMLWLPVLFTKFLPGAVINVATIIHSDEALLAVGFIFTVHFFNT
                     HLRPEAFPMDTVVFTGLVPLEDYMRDRPEEYEEMKRSGELRKRLVKRALSRRYEIGVR
                     VVGGTALGVGLVLITLIIYSMVFRYK"
     gene            complement(6966..7553)
                     /locus_tag="VEC56_05715"
     CDS             complement(6966..7553)
                     /locus_tag="VEC56_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HXV13683.1"
                     /translation="MGRSSVGKLALTVACAAFVMAGAASAADHIYVGAAKCAMCHKKP
                     EQGEQYLKWQESKHAKAYATLATPEAKEVGKKLGVEDPQKDAKCLKCHVTGYAAAAAL
                     KGPKYDATEGVGCESCHGAGNDYAKKSVKDGIMAGTVDPASVGLLKPDQKTCEGCHNE
                     ESPSFKGFDFEKMKAKIAHPIPAERKAKYKTPAGG"
     gene            complement(7654..>7967)
                     /locus_tag="VEC56_05720"
     CDS             complement(7654..>7967)
                     /locus_tag="VEC56_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13684.1"
                     /translation="GTWIMALTGLILWYPVVATNFFPVWIVRVAEVVHFYEAILAVSA
                     IVIWHFFFVIFLPAVYPMSTTWIDGRMPEKEWKEFHAGAPAERGEDGDPQPSGGATSE
                     S"
BASE COUNT         1364 a         2705 c         2530 g         1368 t
ORIGIN      
        1 caccgaccgc cgcgcgcgcc gggaccggcg cagccgcgcg aacgaccgtc gcaaggactc
       61 gtagcgcgag cccgggggcg caccgaatcc gtccatgtgg gcggttcctg tccggccgcc
      121 accggcgtcg aggttgcgcc atcgcacgag atcatcgtcg taacccaata ctgcggttaa
      181 agctaccggt gcttccgggg ccttgcggcc ggccgtggca tgtcgcttgc gctttcaagc
      241 cgcggcgcga ctccttcacc cacgaaggcc ggtccgccta ttccaccgaa ccgtcatgga
      301 cgaacggacg ctctactccc agatcttcga cgccgtgcgc gacccgctca tggtcgtcga
      361 tgccgccggc gacgtgcacg ccgccaacgc ttcggccatc cgattcctcg acgtcgccca
      421 ggacgcgggg ctcttcgggc cgagcgagga cgacggccgt gacaccggct cgcccgtcac
      481 cgccagtgtg ctcgacctgg tgcgccggcg cctgaccgtg cgctcccacc cgctctacgc
      541 gagcgacggc cgcgacctcg acatcgtcgt cgacgtcgaa ccgctcccgg gttcagcggc
      601 cttgctgcac ttccggtcgc ccaccgaacg cctcgcgcgc gagctgtggt cggacgacgc
      661 cgtggccgcg gtcgcgcacg agatcaagaa cccgctttcg gccatgcgca gcgcgctcga
      721 cgtcgtactc aaggatccct ctgcaccgct cctggactcg cagcgccgca tgctgggcgc
      781 gttcgatcgc agcgcacgac gcctcgcgcg cttcgtcgac ggagtgctca acgtttcgcg
      841 cctgcacgcc ggtgccgccc ccgcgccgcg cgcgctggta tcggtgcgcg cactcgtcga
      901 gggcgtggtc gacgagtacc gcgcgctgca tccgcacaag tgtccgcgcc tggagtgcga
      961 ggtggcggag gccggcgcgc gcgcattcgt cgaccaggac ggcgccgaga tcgtgctgct
     1021 gaatctactg gggaacgcgg cgcgcttctc gccgagcgac gccgccgtga ccgtgcgtgc
     1081 ctcccgggcc ggggtcgagg cgatggaaga cgacatgcgc ctggttccgt gggacgtggt
     1141 cggcaagccg cgcctggcgc gcatcgacgt ggaagaccgc ggtctgggaa tgacgcccga
     1201 cgtgctcgcc cacctgttcg atcgctacca cgcgagcgac gagggcgagt tgtccctcgc
     1261 gaccgcgcgc gattccggca cccacctggg gctccacatc tcgcgcgcca tcgccgaggc
     1321 gcacgacggg tggctcaagg tcgagagcag tctgggcgag ggaaccgtcg cgagtgtatt
     1381 cgttcccgaa gacgaagcca ccgcgcgcac gctctcgcgc ctgcgcatcg cggccgattc
     1441 ggcgcggcgc tggcagcgcg cggggcgcga gctggcggtg gtcgcgctgg ccaaggcgtc
     1501 gcccgaaagt tgggaggatc tcgccgtgcg ctgggccgaa gcgccccgcg tcgacccgct
     1561 cgcgtcgacg tgcgcgtcgc gcaaggccct gttgtggccg atcgacgacc atctcggcgt
     1621 cgccatcgtc ccgctcgcgg gcggcaacga cggcccgtcg gtggtgctgg gcgagccggt
     1681 catccgcgcc gacgagttcg cgtggggaat gagcgggttc gtcgcgggct ggtgccgcgt
     1741 cgaggacgcg acctccttcg cgcaggcctt ccacaaggcg gcctcgcgca tggggcgcgc
     1801 gtgcgcgcaa atcgcgcgcg gcggtgttgc cgaagaagca gccacgcccg cgaacgcgga
     1861 ggcgttcgaa gaggcatggg agccggtcgg gatgctgccg gcgatggaga ccacgttgca
     1921 cgtggagaac gaggagtaga gatggatagc ttgcgcattt tgctcgtcga cgacgagcag
     1981 gacatcgtgg acacggtgaa gtacagcctg gagatgaggg gcttcgtggt cgacgtcgcg
     2041 tacgacggtg ccaccgcgct gaccaaggcc cgcaccggcc gctacgccat catgatcctg
     2101 gacgtgatgc tgcccgccaa gaacggctac gaggtctcgc gcatcctcaa ggacgagatg
     2161 acgcgcggcg agctcgcgcc cttcaaggtg ctgctcatca cggcgcgcaa gctcgacaac
     2221 gcgatgcgcg aagagttcgt gtcgacgtgg tcgaaggcgg atgcgtgtct ctacaagccg
     2281 ttcgacctcg agggattgat cgacaagatc tcgggtctct tggacccggt ggcgagataa
     2341 cggattcgcg gggccgcgga gtggcttccc ggcgccaaac cggccaggcg accccgccgt
     2401 ccccgccgcg atgaatgcga cgcgccggag tttccacccg acggcgcgaa acagccgcgg
     2461 gagtttcaca ctcccgcggc ttcgtgtttt ctggccgggg attgcgcttg acaccacgcc
     2521 gtgattgctg aaataaacta caaatctgtg ccgaccggcc gactccactg ctcataagat
     2581 gaaaccaatt caacttccca ggtcgctcaa caaccccatc accatcgtcg gggccggtat
     2641 cgcgcttgtc accggcgtga cactggcgtt cttcctggtt ctcatcgcca cggtcggcga
     2701 atccaatcct tacatcggga tattcgtcta tctggttctc ccgcccatct tcggtttggg
     2761 ggtcatcctg atcccggtcg gggcgctgcg caagcgccgg cgggtgcgaa agcagctcga
     2821 tactgcggcg ctgcgctggc ccgtagtcga cctcaatcag cgtacctatc gcaacgcgtt
     2881 gttcttcttc gtgctcggtc tcggcatgct cacgctgatc agcgtcgtcg gcggttacca
     2941 ggcataccac tatacggagt cggtcgagtt ctgcggcacg acgtgtcata cggtgatgga
     3001 gcccgagcac accgcgtacg ccaactcacc gcacgcgcgg gtttcctgcg cggagtgtca
     3061 cgtcggcgcc ggcgccgggt ggtacgccaa gtccaagctt tccggcgcgt atcaggtgta
     3121 cgcggccgcg ttcaacaagt acccgcgccc catcccgacg cccatcaaga acctgcgccc
     3181 cgcgcaggag acgtgcgagc agtgccactg gccggagaag ttcttcgggg cccagcagcg
     3241 ccaatacaat cattacatgt acgacgaggc caacacccac tggccgatca atctgctgat
     3301 caagaccggc ggcggtgacc ccaagacggg ccagacggcc ggcatccatt ggcacatgaa
     3361 catcggcgtg caggtcgagt acgtagcgcg cgacgagcgc cgccaggaca tcccgtgggt
     3421 gcgcgttacc gaccgcacga ccggacgcgt cacggtctat caggaccagg tcagcccgct
     3481 ctcggccgag gaactggcgg cctcgacggt tcgcaagatg gattgcatcg actgccacaa
     3541 ccggccgagc cacaaattca actcgcccga caacgccatc gaccgcgcca tcctcaccgg
     3601 cgccatcgac ccgtcgctgc cggagatcaa gcgcgtggcg gtggaggcca tgcaggcgcc
     3661 gtacgagacg aaggacgacg cgttccgtgc catcgcgacc aagatcccgg atttctatct
     3721 ccagaactac cccgatatcc tggccgagaa gcgggtggcg atcgacgagg ccgtgctcgc
     3781 gacgcagcac cagttctccc agaatatctt cccggagatg aaggtgcgct gggaagggta
     3841 ccccgacaac atcgggcact tcatcttccc cgggtgcatg cgctgccacg acggcaacaa
     3901 ggtgagcgat gacggttgga ccatcacgcg cgattgcacg gcgtgccacg ccatcctccg
     3961 ccagggcgcc ggcgaaaaca tgcagatggc cgtgacggag gaggggctcg agttcagcca
     4021 cccggacggc ggcgaggact ggcgcgacac caactgtcat gattgccaca cgggaaccca
     4081 accctgaccc ggcccattgt cccgacgatc ggctccggcg cgccgcacgc gttctggcac
     4141 tactgtatgt caccgttcta gtcgccgtta cccccgcggg cgccgccaat ccggccaacg
     4201 atacctgttt cgactgccac gccgaccgcg gtgatcccga ggttcccttc gtcgacggcg
     4261 gcgtcttcgg taaatcgata cacggcgggc tggcgtgcaa cgagtgccac gccgacgtcg
     4321 atcccgacga tctgccgcat gccgaggacc tcaagccggt ctactgcggc ggttgtcacg
     4381 gcgaccagca actcgacttc gacgccagca tccacggcca ggcgctgaac cggaagcaac
     4441 cctacgcgcc gtcgtgcaag gactgccacg gcatccacga catctttgcc ccctcggacg
     4501 agcgctccac ggcgttcaag atgaaggtcc cgtttctgtg cgggcagtgc caccgcgagg
     4561 gcgccccggt ggccaacacc tatcagatct ccgagcacaa catcctggag aactacagcg
     4621 agagcatcca cggcgagggg ctcttcaaga agggactcat ggtgacggcg gcgtgcgccg
     4681 actgccacgg cgcgcacttg atcctgccgc acaccgaacc gcgcgcctcc atttcgccgc
     4741 gcaacatcac cgccacttgc atgaagtgcc acacccggat cgaggatgtc cacaccaagg
     4801 tcatccgcgg cgagttgtgg gagaaggcgc cgggcgccat tcccgcctgc accgactgcc
     4861 acgtgccgca caaggtacga cgggaagcgg tgaccgtgac catttcggac cgcgactgca
     4921 tgaagtgcca cgagcgccac gacgtgttca agatcgcggg cgccgacacc acgtcgatgt
     4981 tggtcgacaa ggacctgctg gactcgtccg cgcacaccaa tattccgtgc gtgaagtgcc
     5041 attccgacgt ggatccgcgg cgccaccgtc cgtgcgaacc gtcggggctg gtggactgct
     5101 cgaactgtca cgcgaagatc gcggatgagt acgcggtcag cggacacggg caggcgcacg
     5161 cgaagggcgt ggccgaggcg ccgtactgca ccgactgtca cggcgggcac ggcgcgctcc
     5221 cgcacctcga cgagtcgtca ccgacctatc gcgcgtcggt ccccgcgctg tgcggcgatt
     5281 gccaccgcgc cgagggcgcc gccggcgcgg tcgaggggct ctcccagcag gatgcgctgg
     5341 cggattattc gcacagcgtc cacgggcgcg gactgacgga gaaggggctc ttgccgagcg
     5401 cggtgtgcat cgactgccat tcgtcgcaca tggtgctcaa gcacaccgac gagcgctcgt
     5461 cggtttcgca ccataatctg ccggcgacgt gcgcgacctg tcaccggggg atctacaagg
     5521 atttcatcaa gagcgtgcac ttcgcgtccg gcgaccagga ccccggcaag ctccccacct
     5581 gcgcgacgtg ccactcgtct cacaccatcg gagaggtcga gggggatgcg ttcgtgcagg
     5641 aagtcacgca ccaatgcggg tcttgccacc acgaactggc ggagacgtat ctcgagacga
     5701 tgcacggcaa ggcctaccgg ctcggctacc tggacgccgc caagtgctcc gactgccacg
     5761 gggcgcactc catcttggac gtcgacgatc ccagttccgc ggtcggctcc cacaacatcg
     5821 tggggacgtg ccagcagtgc cacgaggacg ccaaccagcg tttcaccggg tatctcaccc
     5881 acgcgaccca ccacgatccg gacaagtatc cgttcctgta ctactcgtac tgggcgatga
     5941 cgttcttgct catcggcgtg ttcggtttct tcgggctgca caccctgctc tggctgccgc
     6001 gatcgttccg acacatgctc gagcaccgca aggaggtggc gtggggagag tcccgctact
     6061 acatccggcg cttcacgctg ccccaacgcg tcacgcacct gttcgtgatc gtcagcttcc
     6121 tctcgctggc actcaccggc atgatgctga agttttcctc gatgccgtgg gcggcgttca
     6181 tcgcggatat gctgggaggt gtgaagggcg cgggcatcat ccaccgctgc gcggcggtca
     6241 tgacgttcgg ctacttcgcg ttccacgtcg cgtcgctggt gcgtttcaag cgccaccgcc
     6301 gcctcaaatt aacgcaactc gtcttcggca aagactcgct gatgttcaat cgcaaggacc
     6361 tgtcggactt cgtaggaacc atgaagtggt tcttcaacgc ggggccgcgt ccgcgctacg
     6421 gacgctggac ctactgggag aagttcgact acctggcggt gttctggggc gtggccatca
     6481 tcggcctgtc cgggttgatg ctgtggctgc cggtgctctt caccaagttc ctgcccggag
     6541 cggtgatcaa cgtggcgacg atcatccaca gcgacgaagc actcctggcg gtgggtttca
     6601 tcttcactgt ccatttcttc aacacccacc tgcggcccga ggcgtttccc atggacaccg
     6661 tggtcttcac cgggctggtg cccctcgagg attacatgcg cgaccgcccc gaggaatacg
     6721 aggaaatgaa gcggtccggc gagttgcgca agcgcttggt caagagagca ctgtcgcgtc
     6781 gctacgagat cggcgtgcgg gtggtgggag gcacggccct gggtgtggga ctcgtgctga
     6841 ttacactcat catctactcg atggtgttca ggtacaagta gccggcggcg gggagcgcca
     6901 aaagagatcg ggtcggttcg cggcgcgaac cgacccgatt tttcatccca tcgacaggag
     6961 gagcgttacc cgcccgccgg tgtcttgtac ttggccttgc gctcggccgg aatcgggtgt
     7021 gcgatcttcg ccttcatctt ctcgaagtcg aatcccttga acgacgggct ctcctcgttg
     7081 tggcagcctt cgcaggtctt ctggtcgggt ttcaagagcc ccaccgaagc cggatcgacg
     7141 gtgccggcca tgatgccgtc cttgaccgac ttcttcgcgt agtcgttgcc ggcgccgtgg
     7201 caggactcgc agcccacgcc ctcggtcgcg tcgtactttg gaccctttag cgcggcagcc
     7261 gcggcgtaac cggtcacgtg gcacttcagg cacttggcgt ccttctgggg gtcctcgacg
     7321 ccgagcttct tgccgacctc tttggcctcg ggagtcgcaa gggtcgcgta cgccttcgcg
     7381 tgcttgctct cctgccactt gaggtactgc tcgccctgtt cgggcttctt gtggcacatc
     7441 gcgcacttgg ccgcccccac gtatatgtga tcggcggcac tcgcggcacc cgccatgacg
     7501 aaggccgcgc acgcaaccgt tagcgcgagc ttgcccactg aagaacgtcc catgtgtgtc
     7561 tcctctcttc gcggcaccgc ctaattatac gcgatcgacc gctccaaatc gcactcaaaa
     7621 aaactgggca acccgtcctt gtgcgcagtt ttgctatgat tcactcgtgg cgcccccgct
     7681 cggttgggga tcaccgtcct cgccgcgctc ggccggcgcg cccgcgtgga actccttcca
     7741 ctccttctcg ggcatgcggc cgtcgatcca cgtcgtgctc atgggataca cggcggggag
     7801 aaaaataacg aagaagaaat gccagatgac gatcgccgac accgcgagaa tcgcttcgta
     7861 gaagtgaacg acctccgcga cgcgaacgat ccacacgggg aagaaattcg tcgcgacgac
     7921 tgggtaccag aggatcaatc cggtcagcgc catgatccac gtgcccc
//
LOCUS       DASPGK010000205         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_404379, whole genome shotgun sequence.
ACCESSION   DASPGK010000205 DASPGK010000000
VERSION     DASPGK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404379"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1281)
                     /locus_tag="VEC56_05725"
     CDS             complement(217..1281)
                     /locus_tag="VEC56_05725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV13685.1"
                     /translation="MPVLVVEPWTDARWDAYVASHPRATVYHTSVWIRLVCEIGRYPS
                     LCLVCERDGRPTGVLPLVAVESRLTGNRISTLPFSDTCFALADDEESARALVEEARAL
                     RARRGASFYEMRGAPALREGRDFDPSGIAHNTHFSNYVIPLAGDAEAVRKSFSKSAVR
                     QTITKGARLGVSVRVGDLSDLNVFYGLYLRLRRRHGIPPQPYALFQRILEEMESNGSP
                     SATLYLAEHERVAIAGLIVFRYGGVTYAKYEGADERRRDLVPLYPLLWKTIEDAVNAG
                     DRFYDFGRTAADNPGLNEFKQRWGTTRVDLPYYFDPPREGVSVVRSDSLKYRLFTTAF
                     RRMPASWSVLIGRRIFRHFG"
     gene            complement(1292..2605)
                     /locus_tag="VEC56_05730"
     CDS             complement(1292..2605)
                     /locus_tag="VEC56_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXV13686.1"
                     /translation="MSTKHTIKPAAGRVGVLLPGMGAVATTFIAGVEAVKAGLAKPIG
                     SLTQMGTIRLGKRTDNRTPLVKDLVPLAGLEQLVFAGWDPIADNAYEAAKKAGVLEEK
                     LVDALRPRLEAIKPMPAVFDTRYVKKLDGPNVKKGKNWMELADQVRADIRRFKEEKKL
                     DRVVMIWCGSTEIYLEQKPVHQSIKAFEAGLVANDPDISPSMIYAYAALKERVPYANG
                     APNLSVDMPAMIELAKEMRVPICGKDFKTGQTMLKTVLAPMFKARMLGVAGWYSTNIL
                     GNRDGEVLDDPESFKTKEVSKLGVLEHILQPELNPDLYGNIFHKVRINYYPPRGDNKE
                     GWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLALFLDFAERAGMSGIQEWLSFY
                     FKSPQTAPGLYPEHDLFIQLMKLKNNLRYVMGEELITHLGQEYYE"
     gene            2757..3662
                     /locus_tag="VEC56_05735"
     CDS             2757..3662
                     /locus_tag="VEC56_05735"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXV13687.1"
                     /translation="MEIRQLRYFASTVRLGSVGAAAAEHFVTQPAVSLQLKKLEEELG
                     QKLLQRRGRQLVPTEAGVALAARAEEVIRLLSALEADLSGMKELATGTLRLGNTDAAS
                     VYVLPDVYLEFHTKYAGVRIEIMVTETRRLLEALRARRIEIAIATLPISERGLVVNPI
                     YREELVPVARPDHPLASRRGATLKDLASQDLITYPAGAVTRGMIDAVFAAHGETLRPR
                     MEISSPEAMKRLAQAGLGVSILPRPVVAPELGSKSLKAIALPGVRFEREIGMVYRADD
                     ALSPAARVFRDMIDRRFRRRDQKGR"
     gene            3695..>3957
                     /locus_tag="VEC56_05740"
     CDS             3695..>3957
                     /locus_tag="VEC56_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXV13688.1"
                     /translation="MSTHLHRELEVAKTVARAAGEIVMASYARVVESDVDEKGSNDFV
                     TKVDHASQKVIVDGLRRAFPDDFIVAEESLPPGTNDGRDPHQRR"
BASE COUNT          706 a         1268 c         1284 g          699 t
ORIGIN      
        1 cccggcctgc aacccgaccg cgaccacggg tcactatggc gacatccgcg atctgaccct
       61 gtcgatcacg ggatgcgcgg tggggacaca agaggcgacg tgggggacgg tgaaggcgct
      121 ctaccgagag tagctccgcg caccaacgcg ttcctcgaat cgtcttcagg aatggggatc
      181 ctcgcgcttc ggcgcgagga tcccttttcg ttttcgtcaa ccgaaatgcc gaaagatgcg
      241 ccggccgatc aagaccgacc acgacgccgg catgcggcga aacgcggtcg tgaagaggcg
      301 atacttgagc gaatcgctgc gcacgacact cacgccctcg cgcggcggat cgaagtagta
      361 ggggagatcg actcgggtgg ttccccagcg ctgcttgaac tcgttcaagc ccgggttgtc
      421 ggcggcggtg cgcccgaagt cgtagaagcg atcgccggcg ttcaccgcgt cctcgatggt
      481 cttccagagc aggggataga ggggcaccaa gtcgcgccgt ctttcgtccg cgccttcata
      541 cttcgcatac gttacgcccc cgtatcgaaa cacgatgagc cccgcgattg ccacccgctc
      601 gtgctcggcc aggtacaggg tcgcgctcgg cgagccgttc gattccatct cctcgaggat
      661 gcgctggaac agcgcatacg gttgcggggg gatgccgtgg cgacgccgca ggcgcagata
      721 gagtccatag aagacattca agtcggacaa atcgccaact cgtaccgaca cccccaatcg
      781 cgcccccttg gtgatggtct gacgaacggc ggacttactg aacgatttgc gtaccgcctc
      841 cgcgtcaccc gcgagcggga tcacgtagtt ggaaaagtgg gtgttatgcg cgatcccgga
      901 tggatcgaag tccctaccct cacgaagcgc aggcgcaccc cgcatctcgt aaaacgaagc
      961 tccccgccgc gcacgcagcg cgcgcgcttc ctccaccagg gcgcgcgcgg attcttcatc
     1021 gtcggcgagc gcgaagcacg tatcggagaa gggaagcgtc gagatgcggt ttcccgtgag
     1081 ccggctttcg accgcgacca gcggcagaac gccggtgggg cgaccgtcgc gttcgcagac
     1141 gaggcacagc gacggatagc ggccgatttc gcacaccagc cgaatccaga ccgacgtgtg
     1201 ataaacggtg gcacgcgggt gggacgccac gtaggcgtcc caccgcgcgt ccgtccacgg
     1261 ttcgacgacg agcaccggca cgtcggcggg tctactcgta gtactcttga cccaggtgcg
     1321 tgatgagctc ttcacccatc acgtagcgca ggttgttctt gagcttcatg agctggatga
     1381 acagatcgtg ctccggatac aggccgggcg cggtctgcgg gctcttgaag tagaacgaca
     1441 gccattcctg gatgccggac atgccggcgc gctcggcgaa atcgaggaag agcgccaagt
     1501 cgagcacgag cggtgcggcc aagatggagt cgcggcacaa gaagtcgact ttgatctgca
     1561 tcggatagcc cagccatccg aagatgtcga tgttgtccca gccctctttg ttgtcgccgc
     1621 gcggcggata gtaattgatg cggaccttgt ggaagatgtt cccgtagagg tccggattga
     1681 gctccggctg caaaatgtgc tccaggacgc cgagcttgct cacttccttg gtcttgaacg
     1741 actccggatc gtccagcact tcaccgtcgc ggttaccgag gatgttggtc gagtaccagc
     1801 cggccacacc gagcatgcgc gccttgaaca tgggcgcgag cacggttttc agcatggtct
     1861 ggccggtctt gaagtctttg ccgcagatcg gcacgcgcat ctccttcgcg agttcgatca
     1921 tggccggcat gtcgaccgag aggttggggg cgccattggc gtagggcacg cgctccttga
     1981 gcgccgcgta tgcgtagatc atgctgggcg agatgtcggg atcgttggcg accagccccg
     2041 cttcgaaggc cttgatggac tggtggaccg gcttctgctc gagatagatc tccgtgctcc
     2101 cgcaccagat catcacgacc cggtcgagct tcttctcttc cttgaagcgg cggatgtcgg
     2161 cgcgcacctg gtcggccagt tccatccagt tcttgccctt cttgacgttg gggccgtcga
     2221 gcttcttcac gtagcgcgtg tcgaagacgg cgggcatggg cttgatcgct tccagccgcg
     2281 ggcgcaacgc gtcgaccagc ttctcttcga gcacgccggc cttcttggcc gcctcgtagg
     2341 cgttgtccgc aatcggatcc caccccgcga agaccagttg ctcaaggccg gcgagcggaa
     2401 ccaggtcctt gaccaagggc gtgcggttgt ccgtgcgctt gcccaggcga atcgtcccca
     2461 tctgggtcag cgagccgatc ggcttggcca ggcccgcctt gaccgcctcc acgcccgcga
     2521 tgaacgtcgt ggcgaccgcc cccatgccgg ggagcaggac gccgacccgg cccgctgccg
     2581 gctttatcgt gtgttttgta gacatgtttg gaatctcctc ccggcccggg tcgggcctca
     2641 gtgggcccaa aggcccgatc tcgcgcctcg gcggcgcgcc gtcgatcgcc ccaaggtagc
     2701 cgagcgcttg acataactaa agactaaatt gataatgaat tacattagca attcttatgg
     2761 aaatacgcca gctccgatac ttcgcgtcga ccgtccgctt gggcagcgtg ggcgcggccg
     2821 cggccgagca cttcgtgacc cagccggcgg tgagcctgca actgaagaaa ctcgaggagg
     2881 agctggggca gaagctactc cagcggcggg gtcggcagct cgtcccgacc gaagccgggg
     2941 tggcgctggc cgcccgagcc gaggaagtga ttcggctgct ttccgcgctc gaggcggacc
     3001 tgtcgggaat gaaggagctc gccaccggga cccttcgcct gggcaacacc gacgccgcca
     3061 gcgtgtacgt ccttcccgac gtctacctgg agttccacac gaagtacgcc ggtgtgcgca
     3121 tcgagatcat ggtgaccgag acgcggcgtc tgctggaggc gctgcgcgcg cgccgcatcg
     3181 agattgccat tgccaccctc cccatcagcg agcgcgggtt ggtcgtcaat ccgatctacc
     3241 gcgaggagct ggtgccggtg gcgcgccccg accacccgct cgcgtcgcgg cggggagcga
     3301 ctctcaagga cctcgcgagc caggacctga tcacctatcc agccggtgcc gtgacgcgcg
     3361 gcatgatcga cgccgtcttc gccgcgcacg gggagacgct gcgaccgcgc atggagatat
     3421 cgagccccga ggccatgaag cgactggccc aggcggggtt gggcgtgagc atactcccgc
     3481 gtccggtggt cgcgcccgaa ttggggagca agtcgctcaa ggccatcgcc ctgccggggg
     3541 tccgcttcga gcgggagatc ggcatggtgt accgcgccga cgacgcactt tcgcccgccg
     3601 cgcgtgtttt tcgcgacatg atcgaccggc gtttccgacg acgcgatcaa aagggtcgct
     3661 aagcgagcgg cgatttggca gaatactccc tcacgtgtcc acccatctgc accgagagct
     3721 ggaagttgcc aagacggtcg cgcgcgccgc gggcgagatc gtgatggcgt cctatgcgcg
     3781 cgtggtcgag tccgacgtcg acgaaaaggg cagcaacgac ttcgttacca aggtcgacca
     3841 cgcctcgcag aaggtcatcg tcgacgggct gcggcgggcg ttccccgacg acttcatcgt
     3901 ggcggaagag agtctcccgc ccgggaccaa cgatgggcgc gacccgcacc agcgacg
//
LOCUS       DASPGK010000206         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_638508, whole genome shotgun sequence.
ACCESSION   DASPGK010000206 DASPGK010000000
VERSION     DASPGK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638508"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VEC56_05745"
     CDS             complement(<1..555)
                     /locus_tag="VEC56_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV13689.1"
                     /translation="MHLSEENIQRFLHGELDARSREALSAHVAGCDRCARQLAEAERE
                     EHEIFDLLGRLDHEPPRVDATSIARGRGALAVWGRRAAVFVAAAALAGAAYAIPGSPL
                     PAVFEKVAQWIAREPSTPQSGESVDSLRVASGIAVPARERFALEFVAEQTRGAVALSL
                     TDGSNVAVRAFGDTVTFTTDAGRLT"
     gene            complement(545..1057)
                     /locus_tag="VEC56_05750"
     CDS             complement(545..1057)
                     /locus_tag="VEC56_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV13690.1"
                     /translation="MSRSFHDDFVELFDGHFERLFRYLDRLSGDPDLAADIAQETFMK
                     LHQRGSLPDAPGAWLVSVAMNLFRNAYATRERRSRLLKVVPEAAMYADRSVPPDRGDE
                     SRALEARVRRALDGIPERERQMLLLRAEGFSYREIAEALNLQEASVGTLLARAKRAFK
                     EIYEDRADAS"
     gene            complement(1112..3382)
                     /locus_tag="VEC56_05755"
     CDS             complement(1112..3382)
                     /locus_tag="VEC56_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV13691.1"
                     /translation="MLFWPASVTPLLLAAHAAFIPVTGTVRDADSGMPLAAVIVSLEN
                     LRQTILTDEHGRYSFEAPPGTEHVTVQRFGYAPQTFDAIIPESGALEINVALRREPIM
                     LPPIEVRAPVAIRGLDQPARSVAFPDRSVSQAAMYQHPLSPEPDAFRALGGGEVALDP
                     EAPSGLHIRGGATDQVGYLLDGIPVFSPYHSGGTFSAWNADALSSVDLLAASPLHAAP
                     DALSGVVSANTRAPGRRFQTRGSVSAMQARATIDGPVGRTGVGYLVSVSSAFPGLAIH
                     KDEPSHLRGDNLDWLGKMESPLLGGRMRLTGYQSRNEIDVAAVAEPSDTAAADFTRST
                     LGWNSRSLGGEWSRPLGERAGLLLRAWTATGDASTAWIGPDSLEHLASSRRDDGAVAM
                     IDLAALGGTTTVGGRLQEVESAYSLRPTDDAEQALSFQGVTPMSTFFVEHERPLSATV
                     EVGASFANTFAMGEVYYSPSGRVSWRASPTLLLTGTAARRQQFGQSLRNPESVVSNVF
                     PVDLYVDAGSSGIPVATSDIGILALEHRPKAWLRLGGQAYLRDFESLALVAPNTAEPY
                     ATDGFVVGSGASHGVSLEAGVSREHYGFLASYAYQSVEFRYPAGDYAPGYGSTHTFQA
                     GLVLSPSSSYSVRLGFEGIVGRRTTAALGSVEWEACNILDGGCEFAGSQSRWTEALGA
                     TGLPAYYRLDLGVRKQWDVSLGGRDGHLAVFGTATNLLARMNVLTVAVDPATGQRSNV
                     DMMPPSPLVVGIDWRF"
BASE COUNT          567 a         1168 c         1228 g          517 t
ORIGIN      
        1 ggtcaggcgt cctgcgtcgg tggtgaaggt gacggtgtcg ccgaacgcgc gcacggccac
       61 gttggacccg tcggtgagcg acagggcgac ggcaccacgg gtttgctcgg cgacgaactc
      121 gagggcgaag cgttcgcgcg cgggtaccgc gatcccggaa gccacgcgga gcgagtccac
      181 ggactcgccg ctttgcggtg tcgacggctc gcgcgcgatc cactgcgcga ccttctcgaa
      241 gacggccggc agcggggagc ccggaatggc gtaggcggcg ccggccagcg cggccgcggc
      301 cacgaagacg gcggcgcggc ggccccacac cgcgagtgcg ccccgtccgc gtgcgatcga
      361 cgtcgcgtcg acgcgcgggg gctcgtgatc caggcggccg agcagatcga agatctcgtg
      421 ctcttcgcgc tcggcttccg ccaattggcg agcgcagcgg tcgcacccgg cgacatgggc
      481 cgacaacgct tcccgcgaac gcgcgtcgag ctcgccgtgc aggaaacgct ggatattctc
      541 ttcgctaaga tgcatcggcc cggtcctcgt agatctcctt gaaggcacgc ttggcccgcg
      601 ccagcagcgt tcccacgctg gcttcctgca ggttcagcgc ctcggcgatc tcccggtagc
      661 tgaacccttc cgcgcgcaag agcagcatct gccgttcgcg ctcgggaatc ccatccagtg
      721 cgcgccgcac gcgcgcttcg agggcgcgcg attcgtcgcc gcggtcgggc ggcactgatc
      781 gatccgcgta catggccgcc tccggcacca cctttagcaa gcggctgcgc cgctcgcgcg
      841 tcgcgtacgc gttgcgaaac aggttcatcg caacggaaac gagccacgcg ccgggtgcgt
      901 ccgggagcga cccccgctgg tgcagcttca tgaacgtctc ctgcgcgatg tcggccgcga
      961 gatccgggtc gccggagagg cgatccagat accggaacaa ccgttcgaag tgtccatcga
     1021 agagctcgac gaagtcgtcg tgaaaagacc ggctcatggg actcccggcc ctaggaaccc
     1081 gcgaacggag ggccgtgtga cgccactcgt tctagaagcg ccagtcaatt ccaacgacga
     1141 gcggggaagg aggcatcatg tcgacattgc tgcgctggcc cgtcgcgggg tcgacggcca
     1201 cggtgagcac gttcatgcgc gcgaggaggt tggttgcggt tccgaacacc gcgaggtgcc
     1261 cgtcgcggcc gcccagcgaa acgtcccact gcttgcggac gccgaggtcg agccggtagt
     1321 aggccggcag cccggttgcc ccgagggctt cggtccaccg ggactggctg cccgcgaact
     1381 cgcaaccgcc gtccaggatg ttgcaggctt cccactcgac gctgccgagt gccgccgtcg
     1441 tgcgccgtcc cacgataccc tcgaagccga ggcggacgct gtagctcgac gagggcgaga
     1501 gcaccaagcc cgcttggaag gtgtgcgtcg atccatagcc gggcgcgtag tcgccggcgg
     1561 gataccgaaa ctcgacgctc tggtaggcgt agctcgccag aaacccgtag tgctcgcggc
     1621 tcacaccggc ttcgagcgag accccgtgcg acgcacccga gccgacgacg aacccgtcgg
     1681 tggcgtacgg ctcggcggtg ttgggcgcca ccaaggcgag tgattcgaaa tcgcgcaggt
     1741 acgcctgccc tcccaagcgc aaccaggcct tgggccggtg ctcgagcgcc agtatcccga
     1801 tatcgctcgt cgccaccggg atcccggagc ttcccgcgtc gacgtacaag tcgacgggaa
     1861 acacgttgga cacgacggac tcgggattgc gcagcgactg gccgaactgt tgccggcgcg
     1921 cggcggtacc ggtcaggagc agcgtgggcg atgcgcgcca actcacgcga ccgctcgggc
     1981 tgtaatagac ctcgcccatc gcaaaggtat tcgcgaacga cgcgccgact tcgacggtcg
     2041 cggacaacgg gcgctcgtgc tcgacaaaaa aggtcgacat gggcgtgacg ccctggaatg
     2101 agagcgcttg ctccgcatcg tcggttggtc gaagcgagta ggcgctctcg acttcctgga
     2161 ggcgtccgcc gaccgtcgtc gtgccaccga gtgccgcgag gtcaatcatc gcaacagcac
     2221 cgtcgtcgcg ccggctcgac gcgaggtgct cgagcgaatc cggcccgatc cacgcggtac
     2281 tcgcgtcccc ggtcgcggtc caggcgcgca ggaggagccc ggcgcgttca ccgagcgggc
     2341 gcgaccactc gccacccagc gaccggctgt tccagcccag cgtgctgcgg gtgaagtcgg
     2401 ccgcggccgt gtcgctcggc tccgcaaccg ccgccacgtc gatctcattt cgactctggt
     2461 agccggttag tcgcatacgg ccgccgagca acggcgactc catcttgccg agccaatcga
     2521 gattgtcgcc gcgcaagtgg gacggttcgt ccttgtggat ggcgagcccc gggaacgccg
     2581 agctgacgct caccaaatat ccgacgcccg tgcgtccgac ggggccgtcg atggtggcgc
     2641 gcgcctgcat cgcgctgaca ctcccgcggg tttgaaagcg ccggccgggc gcgcgcgtgt
     2701 tggccgacac gacgcccgag agcgcgtcgg gcgcggcgtg gagcggcgaa gccgcgagca
     2761 gatcgacgct cgagagcgcg tcggcgttcc acgcgctgaa ggtgccgccg gagtgatagg
     2821 gactgaagac cgggatgccg tcgagcaggt agccgacttg gtcggtggcg ccgccgcgaa
     2881 tgtggagccc gctgggcgct tccggatcca gcgccacttc tccgcctccc agcgcgcgga
     2941 acgcgtcggg ctcgggcgag agcgggtgct ggtacattgc cgcctgcgag acggatcgat
     3001 ccgggaacgc caccgagcga gccggttggt cgagcccgcg gatcgctacc ggtgcgcgca
     3061 cctcgatggg aggcagcatg atcggttcgc gccgcagtgc cacgttgatc tcgagcgcgc
     3121 cggactcggg gatgatggcg tcgaaggttt gcggcgcata gccgaaacgc tgcacggtaa
     3181 cgtgctcggt ccccggcggg gcttcgaacg aatagcgccc gtgctcatcg gtgaggatgg
     3241 tctgccggag attctcgagc gagacgatca cggcggcgag cggcattccg ctgtccgcgt
     3301 cgcggaccgt accggtgacg gggatgaacg ccgcgtgggc ggcgagcagc aggggggtaa
     3361 cactcgcggg ccaaaataac atggtccacc tgaaggggcg gtgcgacggc gcggttgcgc
     3421 gcgtcgccta tacgcgtcgc ctacaattgt agcgcatccg gggcgaagtt cgggccgcga
//
LOCUS       DASPGK010000207         7166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1224031, whole genome shotgun sequence.
ACCESSION   DASPGK010000207 DASPGK010000000
VERSION     DASPGK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224031"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VEC56_05760"
     CDS             complement(<1..741)
                     /locus_tag="VEC56_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXV13692.1"
                     /translation="MPATGDIVLCAGELSGDMHAAHLVAALRARGAGRVVGMAGTHCG
                     EAGMDVRHHHRDYAVIGFTGVLSGLPKFYRLERAMRSLIDRASLFIAVDYPGLNLRLC
                     EYARKRGVPVLYYIAPQVWAWGAGRVARMKRCVDTVAVVLPFETEIYREAGIPVEFVG
                     HPFVVDHELPPPRPEDEREWVALLPGSRESEVRHMLPLLVATARALERIRPGARFVVA
                     QSPVVDEEIYRSALARAGVDVRLERDAVE"
     gene            complement(746..1720)
                     /locus_tag="VEC56_05765"
     CDS             complement(746..1720)
                     /locus_tag="VEC56_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV13693.1"
                     /translation="MGLKVGVVGPGRLGRAHVRVLRSLPAVERVACFDTDRERARAVA
                     DEFGALACDSLGALFDSVDAVVVVTTTSAHAEVALAAIAAGKDVFVEKPITGTLAEGE
                     QVVRAARKSNRILQVGHVERFNGAIEAVRPRIGTPLFIEVHRLAPFTIRGADVSVVMD
                     LMIHDLDLLGLLVGERPADVRAKGAAIITAEPDIVNARLEYEGGCVANVTASRVTAAP
                     MRKVRVFSAAGYFSIDLLAGVATHYQKGPRLAEQLASLRGGASVGLADMVRVEQLRGD
                     GVEPLVKELDAFCQALAARTAPPVTGEDGLEAMRLATVILDRVGSARV"
     gene            complement(1726..2520)
                     /gene="lpxA"
                     /locus_tag="VEC56_05770"
     CDS             complement(1726..2520)
                     /gene="lpxA"
                     /locus_tag="VEC56_05770"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXV13694.1"
                     /translation="MRSYNLKKTEIHPTAVIHPWARIGEGVKIGPYTVIGENVEIGDE
                     CEIGSNVLIEGATKIGRNNKFFHGASVGTAPQDLKYHGETTFLTIGDDNVFREFVTLN
                     LATGEGESTVIGSRCLLMAYVHVAHNCVIGSEVILANAVNLAGHVSIDDFVTIGGLTP
                     VHQFVRIGKYAFIGGGSRIERDVPPFIKLAGNPPQIYGLNSIGLERRGFDAERRSQIK
                     SIYKLLYRSDLNVTQVLEALDGDDWDGADAAEMAQFLRHCERGITK"
     gene            complement(2543..3856)
                     /locus_tag="VEC56_05775"
     CDS             complement(2543..3856)
                     /locus_tag="VEC56_05775"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HXV13695.1"
                     /translation="MVRQQRTIAKEFVIEGLGLHTGNTVKMRVKPAPPGTGIKFIRVD
                     LNPAVEIPAVVTSIPDDATSYRNTSISTGPAQIHTVEHVLATAYGLGIDNLVIDIDAN
                     EPPEPANGTYASYVRAFEQAGTVNQGAPADLFTVDRAVTLKENGIEIHAVPADDFRVS
                     FTIQYDNPQIGTQYASFTVSPDVFEKEIAPARTFVMFDDVERLRAMGLIKGGNLGNAL
                     VFTRDGVMNEEQLRFPDECVRHKILDLIGDLSLLGAPIRGHVHAVRSGHAANVRFVRK
                     LWEIHQGRKRAAGATETGVWDINLIQEIMPHRYPFLLVDRIVELEDRKRVVGIKNVTI
                     NEPFFNGHFPGHPIMPAVLIIEAMAQTGGVMLLNTVDDPKRYLVYFTRVDKAKFRRPV
                     LPGDQLRFELEMKSLRRGLCTMQGYAYVDGKLAAEAELSSVIVER"
     gene            complement(3828..4895)
                     /gene="lpxD"
                     /locus_tag="VEC56_05780"
     CDS             complement(3828..4895)
                     /gene="lpxD"
                     /locus_tag="VEC56_05780"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXV13696.1"
                     /translation="MGARSYRLYELAELVGGTVDGDGQTVIRGVAGIREAEPGEITFI
                     ANPKYEEFLGVTRASAVITGRALAAPKPVIRIDNPYYAYLQVLNLFAADVAVRYPRGV
                     HESAVVDPSASLGQNVAVGPLCHVGKNARVGDNTTLVSGVFVGEAVEIGTDCLIYPCV
                     TIREGTVVGNRVILQPGVVLGADGFGYAKNGSTHHKVPQIGRVVIEDDVELGANTCVD
                     RATTGDTRVRRGSKIDNLVQIAHNVVVGEDNVVAAQAGISGSSELGSHVVLGGQAGLA
                     GHITVGDHSMVAAQAGVTKDVRPRTVVSGYPAREHTLAKRLWAHTALLPDLFKRVKEL
                     ERRLRELEEGGRRGPPTANDR"
     gene            complement(4931..5461)
                     /locus_tag="VEC56_05785"
     CDS             complement(4931..5461)
                     /locus_tag="VEC56_05785"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXV13697.1"
                     /translation="MMRKLVVMLIGAFLVVGGAAVSALAQEVKIGYIDSIKIFAENKE
                     TQEAERLYRQDVQQWEAQKQRMEQELARMGEELNAQSPMLSEEKKMERRLEMNRKMDE
                     YKRFMEETFGDNGLAAKRNKELTQPIVDKINRIIEALAIELKYTMVFDVANANIVYAD
                     KKLDMTDLVLTRLNQR"
     gene            complement(5496..>7166)
                     /gene="bamA"
                     /locus_tag="VEC56_05790"
     CDS             complement(5496..>7166)
                     /gene="bamA"
                     /locus_tag="VEC56_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXV13698.1"
                     /translation="RKSIKTKPKGFLRGGDFSPKQLAEDREAIIQLYKTYGFLDATVN
                     EPELEFASNGKDVDIYFTIGEGRQYYVGDIDWKGNELLPDSVIAGNIRFRKGQVFNDI
                     ALTQMQFDLANLYGDRGYIYATVTPVKKVRGNVIDLTFEIQQGNLAHVREINIAGNTK
                     TWEEVIRRELVLMPGDVFSSNRLRRSLREVFNLGFFAGPPEPQVTQANDEGDVDLTLR
                     VEEKSAGQFRVGAGFSQLNRVSGFIGITEPNFLGRGYRVSGNWEFSRSRQDVSLSFTQ
                     PWAFGTPTELSLSVYAANQDRVQQQFYSDRRTGFSIRVGRPFPWLDYTTVSVRYSLEE
                     VELADFTSGYNGPLRFEQWPQTTSAIGVTFYRNSTDNPFHPTLGTRTTLNARWTGAAL
                     GGDVDIQRYEIDMSWYQSLVWKFVLELRENLGVVDGYTSSVAVPDYERFRLGGNRRYG
                     VRGYDFYEITPRGNDTFTGGLFYSITVAQIEFPIVAPTVYGRTFLDMGGTWNSFGTSE
                     WGDLFKGAGLGVVIELPMIGLFGFDYAYGFDRFGGGRWEPHITFGSSF"
BASE COUNT         1198 a         2370 c         2288 g         1310 t
ORIGIN      
        1 ctcgaccgcg tcgcgctcca gccgcacgtc gacccccgcg cgcgcgagcg cgctgcgata
       61 aatttcctca tcgaccaccg gactctgcgc gacgacaaag cgcgcgccgg gacgaattcg
      121 ctccagcgcg cgcgccgtcg cgaccaggag cgggagcatg tggcgaacct cggactcgcg
      181 gctccccggg agcagggcga cccattcgcg ctcgtcctcg ggtcgcggcg ggggcagttc
      241 gtggtcgacc acgaaggggt gaccgacgaa ttcgaccggt atccccgctt ctcggtagat
      301 ctcggtctcg aacggcagca ccaccgccac cgtgtcgacg caccgcttca tgcgcgcgac
      361 acggcccgcg ccccacgccc acacttgcgg cgcgatgtaa tagagcaccg gcacgccgcg
      421 cttgcgggcg tactcgcaca ggcgcaggtt gagcccggga taatcgacgg cgatgaagag
      481 ggacgcgcga tcgatgagcg acctcatcgc acgctctagt cgatagaact tggggaggcc
      541 cgagaggaca cccgtgaatc cgatgacggc gtaatcgcgg tggtggtggc gcacgtccat
      601 gccggcttcg ccgcagtgcg tgcccgccat ccccaccacg cgcccggccc cgcgcgcgcg
      661 cagcgccgcc accaagtgcg ccgcgtgcat gtcgcccgag agctctccgg cgcacagcac
      721 gatgtcgccc gtcgccggca tgcgactaga cgcgggccga cccgacccgg tcgaggatca
      781 cggtggccag ccgcatcgct tccaggccgt cctcccccgt aaccggcggt gccgtgcgcg
      841 ccgccagcgc ttggcagaac gcgtccagct ccttgaccaa cggctccaca ccgtcgccgc
      901 ggagctgctc gacgcgcacc atatccgcga gcccgaccga tgcgccgccg cgcaacgacg
      961 cgagctgctc cgccagacgc gggcctttct gatagtgggt ggcgacaccg gccagcaggt
     1021 cgatggagaa gtagccggcc gcggagaaga cgcgcacctt tcgcatcggc gcggcggtga
     1081 cacggctcgc cgtcacgttg gccacgcagc ccccttcgta ctcgaggcgc gcgttgacga
     1141 tgtcgggctc ggcggtaatg atcgccgccc ctttggcgcg cacgtccgcg ggccgttcgc
     1201 ccaccagcag gcccaagagg tcgaggtcgt ggatcatgag atccatcacc accgagacgt
     1261 cggcgccgcg gatggtgaac ggggccagcc ggtggacttc gatgaacaac ggcgtcccga
     1321 tgcgcgggcg caccgcctcg atggcgccgt tgaagcgctc gacatgtccc acttggagaa
     1381 tacgattcga ctttcgggcg gcgcgtacca cttgctcgcc ttcggcgagc gtccctgtga
     1441 tcggtttctc gacgaagacg tccttgccgg ccgcgatcgc ggccagtgcg acttcggcgt
     1501 gcgcagaggt cgtggtcacc accaccaccg cgtcgacgga gtcgaacaag gcccccaggg
     1561 aatcgcaggc gagggcgccg aactcgtcgg ctaccgcgcg ggcccgctcg cggtcggtgt
     1621 cgaagcacgc cacccgctcc accgccggca gcgagcgcaa cacacggacg tgggcacggc
     1681 ccagacgacc cgggcccacg actccgactt tcagtcccac cgcggctact tggtgatgcc
     1741 gcgctcgcag tggcgcagga actgggccat ctcggccgcg tcggcgccgt cccagtcgtc
     1801 gccgtcgagc gcttccagca cctgcgtcac gttgaggtcg cttctataga ggagcttgta
     1861 gatcgatttg atctgcgagc gccgctcggc gtcaaagccc cggcgctcga gcccgatcga
     1921 gttcaacccg taaatctggg ggggattccc cgccagcttg atgaagggtg gtacgtcgcg
     1981 ctcgatccgg ctcccgccgc cgatgaacgc gtacttgccg atgcgcacga actggtgcac
     2041 gggcgtcagg ccgccgatgg tcacgaagtc gtcgatgctg acgtggccgg ccaggttgac
     2101 cgcgttggcc aggatgacct cgctgccgat gacgcagttg tgcgccacgt ggacgtacgc
     2161 catcaggagg cagcggctgc cgatcacggt cgactcgcct tcgccggtgg ccagattcaa
     2221 ggtgacgaac tcgcgaaaca cgttgtcgtc gccgatggtg aggaacgtgg tttcgccgtg
     2281 gtacttcagg tcttggggag cggtacccac gctggcccca tggaaaaatt tgttgttgcg
     2341 gccgatcttg gtggcgccct cgatcagcac gttggacccg atctcgcact cgtcgccgat
     2401 ctcgacgttc tcgccgatca cggtgtaggg cccgatcttg accccctccc caatgcgcgc
     2461 ccacgggtgt atgacggcgg tcggatgaat ctcggtcttc ttcagattgt agctgcgcac
     2521 cgaagtgccc ccttcgtgtt tgttaccgct ccacgatgac ggaagatagt tcggcctctg
     2581 ccgctagctt gccgtcgacg taggcgtaac cttgcatcgt acaaagaccc cgccgaagcg
     2641 acttcatctc gagttcgaaa cgaagctgat cgccgggtaa tacggggcgt cgaaacttgg
     2701 ctttgtcgac gcgcgtgaag tacaccaggt acctcttcgg gtcgtcgacg gtgttgagca
     2761 acatgacccc gccggtctgc gccatcgctt cgatgatgag aacggcgggc atgatgggat
     2821 ggcccgggaa atgtccgttg aaaaacggtt cgttgatggt gacgttcttg atgccgacca
     2881 cgcgcttgcg atcctcgagc tcgacgatgc gatccacgag gaggaacggg tagcggtgcg
     2941 gcatgatctc ctggataaga ttgatatccc aaacaccggt ctcggtggcg cccgcggcgc
     3001 gtttgcgccc ttggtggatc tcccagagct tgcgcacgaa acgcacgttg gcggcgtggc
     3061 cggagcgaac cgcgtgcacg tggccgcgaa tgggtgcccc caagagcgag aggtcaccga
     3121 tcaagtcgag gatcttgtgg cgcacgcact cgtcggggaa gcgcaactgc tcctcgttca
     3181 tgaccccgtc gcgcgtgaac accagcgcgt tgccgagatt tccgcccttg atgagaccca
     3241 tggcgcgcag gcgctcgacg tcgtcgaaca tcacgaaggt gcgcgcgggc gcgatctcct
     3301 tctcgaacac gtcgggtgag acggtgaagc tcgcgtactg cgtgccgatt tggggattgt
     3361 cgtactggat ggtgaagctc acgcgaaaat cgtccgcggg gacggcgtgg atctcgattc
     3421 cgttctcctt gagggtcacc gcgcggtcga cggtgaagag gtccgcgggc gccccttggt
     3481 tgacggtccc cgcctgctcg aacgcacgca cgtacgaggc gtaggtcccg ttcgcgggtt
     3541 ccggcggctc gttggcgtcg atgtcgatga cgaggttgtc gatgccgagc ccgtaggcgg
     3601 tggcgagcac gtgctcgacg gtatggatct gggccgggcc ggtggagatc gaggtgttgc
     3661 ggtacgacgt cgcgtcatcg gggatgctcg tcaccacggc cggaatctcg acggccggat
     3721 tcaagtcgac gcgaatgaat ttgatcccgg tccccggggg cgccggcttg acccgcatct
     3781 tcaccgtgtt gccggtgtgg aggccgaggc cttcgattac gaactcctta gcgatcgttc
     3841 gctgttggcg gaccacggcg gcctccttcc tcgagctcgc gcaggcggcg ctccagctcc
     3901 tttacacgtt tgaacaggtc gggcagcagg gcggtatggg cccacaggcg cttggcgagg
     3961 gtgtgctcgc gcgccggata gccggacacg acggtcctcg gtcgtacgtc cttggtcacg
     4021 ccggcctggg ccgccaccat ggagtggtcg cccaccgtga tatgccccgc caggcccgct
     4081 tgaccaccca gtacgacatg gctcccgagc tcgctgctgc ccgagatgcc ggcctgcgcg
     4141 gccacgacgt tgtcttcgcc gacgacgacg ttgtgcgcga tctgaaccaa gttgtcgatc
     4201 ttactcccgc ggcgcacgcg cgtgtcgccc gtggtggcgc gatcgacaca ggtgttggcg
     4261 cccagctcga cgtcgtcctc gatcacgacg cggccgatct ggggcacctt gtggtgggtc
     4321 gagccgttct tcgcgtatcc gaagccgtcg gcgcccagca ccacgcccgg ctgcaggatc
     4381 actcggttgc cgaccaccgt cccctcgcga atggtgacgc agggatagat caaacagtcg
     4441 gtcccgatct caaccgcctc gcccacgaac accccgctca ccagggtcgt gttgtcgccg
     4501 acgcgcgcgt tcttccccac gtggcacagc gggcccacgg cgacgttctg ccccaggctc
     4561 gcgctggggt ccacgaccgc cgactcgtgc acgccccgcg gatagcggac cgcgacatcg
     4621 gcggcgaaca ggttcaagac ttggagatag gcgtagtagg gattgtcgat ccggatcacg
     4681 ggcttggggg ccgccagggc gcgcccggtg atgaccgcgg acgcgcgcgt gacacccagg
     4741 aattcttcgt acttgggatt ggcaatgaac gtgatctcgc cgggctccgc ttcgcgaata
     4801 ccagcgacac cgcggatcac cgtctggcca tcgccatcga cggttccgcc cacgagctca
     4861 gcgagctcgt acagcctgta cgaacgggca cccaaaaatt ctgcacctgc ctaacgccga
     4921 ctagcggtca ctaccgctgg tttaagcgcg tcagcacgag gtcggtcatg tcgagcttct
     4981 tgtccgcgta tacgatgttc gcgttcgcga cgtcgaagac catggtgtac ttgagttcga
     5041 tcgcgagcgc ttcgataatt ctatttatct tgtcgacgat cggctgggtg agctctttgt
     5101 tgcgcttcgc cgccagtccg ttgtcgccga acgtttcttc catgaaacgc ttatactcgt
     5161 ccatcttgcg gttcatctcg agccggcgct ccatcttttt ctcttccgag agcatggggc
     5221 tctgcgcgtt cagctcttcg cccatgcgcg cgagctcctg ctccatgcgc tgcttctggg
     5281 cttcccactg ttggacgtcc tgacgataca ggcgttcggc ttcttgtgtc tccttgtttt
     5341 ctgcaaagat tttgatcgag tcaatgtacc caatcttgac ctcctgggca agcgccgaaa
     5401 cagccgcgcc cccgaccacc aagaaggccc cgattaacat cacaaccagc ttgcgcatca
     5461 tcagttgcac tctccttcca aggtccattt catcgctaga aagacgaacc aaaggtaata
     5521 tgcggctccc agcgaccgcc cccgaagcgg tcgaacccgt acgcgtagtc aaagccgaac
     5581 agcccgatca tgggaagctc gatgacgacc ccgagcccgg cgcctttgaa gaggtcgccc
     5641 cactcgctcg tgccgaagct gttccacgtg ccgcccatat ccaagaacgt gcgcccgtat
     5701 acggtcggcg ccacgatcgg gaactcgatc tgcgccaccg tgatgctgta gaagagaccg
     5761 ccggtgaacg tgtcgttgcc gcgcggcgtg atctcgtaga agtcgtaccc gcgtaccccg
     5821 tagcggcggt tgccgcccag gcgaaagcgc tcgtagtccg ggaccgcgac cgaactcgtg
     5881 tacccgtcca ccacgcccag gttctcgcgc agctcgagga cgaatttcca caccagcgac
     5941 tgataccacg acatgtcgat ttcatagcgc tggatgtcga cgtcgccgcc cagcgcggca
     6001 ccggtccagc gcgcgttgag cgtggtgcgc gtccccaggg tggggtggaa cgggttgtcg
     6061 gtcgagttgc gatagaacgt caccccgatc gcgctcgtgg tctgcggcca ctgttcgaag
     6121 cgcagcgggc cgttgtagcc ggacgtgaag tcggccagct cgacttcctc caggctgtag
     6181 cgcaccgaca ccgtcgtgta gtcgagccac gggaagggac gcccgacgcg aatcgagaag
     6241 cccgtgcgcc ggtccgaata gaattgctgc tgcacgcggt cctggttggc ggcgtacacc
     6301 gagagcgaga gctcggtcgg ggtcccgaac gcccacggct gcgtgaacga caggctgacg
     6361 tcctggcgtg aacgcgagaa ctcccagttg ccgctgaccc gatagccgcg tcccaggaag
     6421 ttgggctcgg tgatgccgat gaacccgctg acacggttga gctgcgagaa gccggccccc
     6481 acgcggaact ggcccgcgga cttctcttcc acccgcaggg tcagatcgac gtccccctcg
     6541 tcgttggcct gcgtcacctg cggctccggc ggcccggcga agaagcccag gttgaacacc
     6601 tcgcgcagcg agcgccgcag gcggttgctc gagaagacgt cgcccggcat gagcaccaat
     6661 tcgcgccgga tcacttcctc ccaggtcttg gtgttgcccg cgatgttaat ctcacgcacg
     6721 tgggccaggt tgccctgctg gatctcgaac gtgaggtcga tgacgttgcc gcgcaccttc
     6781 ttgaccggtg tgaccgtcgc gtagatatag ccgcgatcgc cgtacaggtt ggcgaggtcg
     6841 aactgcatct gggtcagcgc gatgtcgttg aacacttgtc ccttgcggaa gcggatgttc
     6901 ccggcgatca cgctgtccgg gaggagctcg ttacccttcc aatcgatgtc gcccacgtag
     6961 tactgccggc cctcgccgat cgtgaagtag atgtcgacgt ccttcccgtt ggaagcgaac
     7021 tcgagctcgg gctcgttcac ggtggcgtcc aggaatccat aggtcttgta cagttggatg
     7081 atcgcctcgc gatcctcggc cagttgcttg gggctgaagt cgccgccgcg taggaaaccc
     7141 ttgggtttgg tcttgatcga cttgcg
//
LOCUS       DASPGK010000208         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_404945, whole genome shotgun sequence.
ACCESSION   DASPGK010000208 DASPGK010000000
VERSION     DASPGK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404945"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VEC56_05795"
     CDS             complement(<1..1547)
                     /locus_tag="VEC56_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV13699.1"
                     /translation="MNRTVFATLAIALAPSGAWAVTPTPPVAAKRPHVTVTHGDSLRD
                     DYYWLREKTSPDVLRYLEAENAYCDAVMAPTIGFQDALYKEMLARIQETDVDVPYRDG
                     GYFYYTRTEEGKQYPIFCRKKGSLNAREEVTLDVNAMAAGHEYFGVDAYEVSDDGNLL
                     AFSTDTTGFRQYDLSVKDLRTGAILPDRAERVTTVAWGADNRTLFYAQEDPVTQRWHR
                     VYRYVLGSNTHELLFDETDEQYDTWVTRTRSGAYVVIATGSSTTSEAWVIRADRPLDA
                     MASIAPRREGHEYYLDHAGERFLIRTNDRGRNFRLVSSPVAQPGEKNWKEVVAHRDDV
                     MLEDVDAFAGHTVLVEREQGVPYLRVIDAASGKAHRVEFPEPVYVVEPSDNHEFETTA
                     LRFVYESMVTPESVFDYDVKTRERTLLKQRPVLGGYDPGRYRCERVYAQAADGTRIPI
                     SLVYRGHERPRNRPLLLLGYGSYGYASEPYFQSNRLSLLDRGVVYGIAHIRGGGEMGK
                     RWHDGGR"
     gene            complement(1584..2504)
                     /locus_tag="VEC56_05800"
     CDS             complement(1584..2504)
                     /locus_tag="VEC56_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HXV13700.1"
                     /translation="MARLRAGSLAKRHRVVDGARFRLSDHDPADTGSIASKERAQKLL
                     ARGIRRLRDAQERLYAQDTWAVLLVFQAMDAAGKDGAIEHVMSGVNPQGCQVFSFKAP
                     SEEELDHDFMWRTSKNLPERGRIGIFNRSYYEEVLVVRVHPEILQRQKLPPKLVTRNV
                     WKHRFEDMVAFERYLARNGVVVLKFFLHVSKEEQQRRFLSRIDEPAKQWKFSGADVRE
                     RGFWDDYMRAYDDMIRHTASAHAPWYVVPADNKWYTRLVVASAVIDALDELHLAFPRV
                     GAEKKRELAEAKRALEKEALRGAKSRGRRK"
     gene            complement(2506..3375)
                     /locus_tag="VEC56_05805"
     CDS             complement(2506..3375)
                     /locus_tag="VEC56_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV13701.1"
                     /translation="MSTPRLPDATHIGYVHLRVRDLDRTLAFYLDVLGLKMVSGGAHT
                     ATLSANGRGPGLLVLTEKKDAPGRPPRATGLYHVAFRVPNRRALAMTIAHLRETQWPL
                     DGFADHDVSEAVYLSDPEGNGIEVYADRPRETWPLRGGVVEMVTVPLDLEGLLRELAD
                     WPGAWEGLDPATDVGHIHLRVSDLDRTETFYSGVLGFDVTQRSLPGALFLSAGGYHHH
                     IGTNVWHSENGVRPPADAPGLISYSIVVADPVYLPTLEARLRDASVPVRTDDDGGVRA
                     ADPDGVFIELVTA"
     gene            complement(3386..>3691)
                     /locus_tag="VEC56_05810"
     CDS             complement(3386..>3691)
                     /locus_tag="VEC56_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HXV13702.1"
                     /translation="LQVYPAPEITVTFDPNVCVHSGVCLVSLPAVFDVRRARWIDTDA
                     GTPDRIAHAIDRCPSGALQYYRNRSRNPIAASRVRAFVALNATALAHARSATPLWER"
BASE COUNT          556 a         1221 c         1266 g          648 t
ORIGIN      
        1 atgcgcccgc cgtcgtgcca gcgcttgccc atctcgccgc cgccgcggat atgcgcgata
       61 ccgtagacga ccccgcggtc gagcaggctc aggcggttgg actggaagta cggctcgctc
      121 gcgtatccgt acgacccgta accgagcagc aagagcggtc ggttgcgcgg gcgctcatga
      181 ccgcgataca cgagggatat cgggattcgc gtcccgtcgg cggcttgcgc gtagacgcgc
      241 tcgcagcggt accgcccggg gtcatagccg cccaggaccg gacgctgttt gagcagcgtg
      301 cgctcccgcg tcttgacgtc gtagtcgaac accgactcgg gtgtcaccat cgactcgtac
      361 acgaatcgca aggccgtggt ctcgaactcg tggttgtcgc tcggctcgac gacgtatacc
      421 ggttccggga actcgacgcg gtgcgccttt ccggacgcgg cgtcgatcac gcgcaggtac
      481 ggcacgcctt gttcgcgctc caccaaaacg gtgtggccgg cgaacgcgtc cacgtcctcg
      541 agcatcacgt cgtcgcggtg cgcgacgact tccttccagt tcttttctcc cggctgcgcg
      601 accggggagg acaccagccg gaaattgcgt ccgcggtcgt tggtgcgaat caagaatcgc
      661 tcgccggcgt ggtcgaggta gtactcgtgc ccttcgcggc gcggcgcgat cgacgccatc
      721 gcgtccagcg gccggtcggc gcggattacc cacgcttcgc tggtggtcga gctgccggtc
      781 gcaatcacga cgtacgcgcc gctgcgcgtg cgcgtcaccc acgtatcgta ctgttcgtcc
      841 gtctcgtcga acaggagttc gtgcgtgttc gacccgagca cgtagcggta tacgcggtgc
      901 cagcgctgcg tcaccgggtc ttcctgggcg tagaacaacg tgcggttgtc cgccccccac
      961 gccaccgtgg ttacgcgctc cgcgcggtcg ggcaggatcg cgcccgtgcg caggtccttc
     1021 acggacaggt cgtactggcg aaagccggtg gtatcggtgc tgaacgcgag caggttgccg
     1081 tcgtcgctca cctcgtaggc gtcgacgccg aaatactcgt gtcccgctgc catcgcgttc
     1141 acatcgaggg tcacttcctc acgcgcgttc aggctcccct tctttcggca aaagatcggg
     1201 tactgcttcc cctcctcggt gcgcgtgtaa tagaagtagc cgccgtcccg gtacggcacg
     1261 tcgacgtcgg tctcctggat gcgggccagc atctccttgt agagtgcgtc ctggaacccg
     1321 atggtgggcg ccatgaccgc gtcgcaatag gcgttctccg cctccaggta acgcagtacg
     1381 tcggggctgg tcttttcgcg cagccagtag tagtcgtcgc gcagcgaatc gccgtgcgtg
     1441 acggtgacgt gtggtcgctt cgcggcgacc ggcggggtgg gtgtcacggc ccaggcgccg
     1501 ctcggcgcca aggcgatggc aagggtcgcg aacacggtgc gattcatggt gctcctcttt
     1561 cgtttcgccg gcgggcgttt cgcctactta cgccgcccgc gcgatttcgc tccgcgcagg
     1621 gcttccttct cgagcgcgcg cttggcctcg gcgagctcgc gcttcttttc cgcgcccacg
     1681 cgcgggaacg ccaggtgaag ctcgtcgagc gcgtcgatga cggcactcgc caccaccagc
     1741 cgcgtgtacc acttgttgtc ggccggcact acgtaccacg gcgcgtgcgc ggacgcggtg
     1801 tgccggatca tgtcgtcgta cgcgcgcatg tagtcgtccc aaaatccgcg ctcgcgcacg
     1861 tcggcgcccg agaacttcca ttgcttggcg ggctcgtcga tacgggacag gaaacggcgc
     1921 tgctgctcct ccttcgacac gtggaggaag aacttgagca ccacgacacc gttgcgcgcg
     1981 aggtagcgct cgaacgcgac catgtcctcg aagcggtgct tccagacatt gcgcgtcacg
     2041 agcttggggg gcagcttctg gcgctggagg atctcggggt ggacgcgcac caccagcact
     2101 tcttcgtagt acgaccggtt gaagatcccg atgcgcccgc gctccggaag gttcttgctc
     2161 gtgcgccaca tgaagtcgtg gtcgagctct tcctcggagg gcgccttgaa cgaaaacacc
     2221 tggcagccct gcggattgac acccgacatc acgtgctcga tggcgccgtc cttgccggcg
     2281 gcgtccatgg cctggaagac caagagcacg gcccaggtgt cctgggcgta gagacgctcc
     2341 tgcgcgtcgc gcaggcgccg gatgccgcgc gcgagcagct tctgcgcgcg ctccttgctg
     2401 gcgatcgatc cggtgtcggc gggatcgtgg tcggacagtc gaaagcgcgc gccgtccacg
     2461 acgcgatggc gtttcgccag tgagcctgcg cgaaggcgtg ccatgctagg cggtcacgag
     2521 ctcgatgaag acgccgtcgg ggtcggcggc gcgcacgccg ccgtcgtcgt cggtgcgcac
     2581 gggtaccgac gcgtcgcgca ggcgcgcttc cagcgtgggc aggtagaccg ggtcggccac
     2641 gacgatgctg tacgagatca agcccggcgc gtccgcgggc ggccgcacgc cgttctcgct
     2701 gtgccacacg ttggtgccga tgtggtggtg gtagccgccc gccgacaaaa acaaagcgcc
     2761 cgggagcgat cgctgcgtca cgtcgaagcc gagcacgccg gaatagaagg tctcggtacg
     2821 atccaggtcg gacacgcgca ggtggatatg cccgacgtcg gtcgcggggt ccaggccctc
     2881 ccaggccccg ggccagtcgg cgagctcgcg caggaggccc tccaggtcga gcggtaccgt
     2941 caccatctcg accacaccgc cgcgcagggg ccacgtctcg cgcggccggt ccgcgtacac
     3001 ttcgatgccg ttgccctccg ggtcggacag gtacacggcc tcgctcacgt cgtggtcggc
     3061 gaagccgtcg agcggccact gcgtctcgcg caggtgcgcg atcgtcatgg cgagcgcgcg
     3121 ccggttggga acgcggaatg cgacgtggta caacccggtc gcgcgcggcg gtcgcccggg
     3181 cgcgtctttc ttttcggtca ggaccaacag gccgggcccg cgcccgttgg ccgaaagggt
     3241 cgcggtatgc gcgccgccgc tgaccatctt cagccccaga acgtcgaggt agaacgccag
     3301 cgttcgatcg aggtcgcgca cgcgcaggtg cacgtagccg atgtgggttg cgtcggggag
     3361 tcgaggcgta ctcatgggct cgttcttagc gctcccagag cggggtcgcc gatcgcgcgt
     3421 gggcaagggc cgtggcgttg agggcgacaa acgccctaac tctagatgcg gcaattggat
     3481 tccggcttct attacggtag tactgaagcg ctcccgaggg gcaccggtcg atcgcgtgcg
     3541 cgatgcgatc cggggtcccg gcatcggtgt cgatccaacg cgcgcgccgc acgtcgaaga
     3601 cggcggggag cgagaccagg cataccccgg agtggacgca gacgttgggg tcgaacgtca
     3661 ccgtgatctc gggggcggga tagacttgca g
//
LOCUS       DASPGK010000209         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_289235, whole genome shotgun sequence.
ACCESSION   DASPGK010000209 DASPGK010000000
VERSION     DASPGK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289235"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="tsaD"
                     /locus_tag="VEC56_05815"
     CDS             complement(<1..927)
                     /gene="tsaD"
                     /locus_tag="VEC56_05815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXV13703.1"
                     /translation="MIVLGIETSCDDTSLALYSRERGLLSHVTASQLVHEDFGGVVPE
                     IASRQHMRTLLSVYDAVLARAKVGANDLGGVGVSNGPGLVGSLLVGVGFAKSLALGLQ
                     RPVVGVHHIEAHVLSNELGGEPLQCPCVVLVVSGGHTSLYRVERIGRYELVGGTRDDA
                     AGEAFDKIAKLLGLGFPGGPAIQRAAEAGDATAIDFPRAMLDRAGFDFSFSGLKTAVR
                     QYVDARGELTPEMVANIAASAQAAIVDVLVERTIQCARSLGVRHVYLAGGVAANRPLR
                     AKMHATADAAGIAYHAPLLEFCTDNGAMVARTA"
     gene            complement(973..1311)
                     /locus_tag="VEC56_05820"
     CDS             complement(973..1311)
                     /locus_tag="VEC56_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXV13704.1"
                     /translation="MKKIEAFIKPFKLDDVKSALMEIGVQGLTVSEVKGFGRQKGHTE
                     LYRGSEYRVDFLPKSKLEIFVADADVEKVVETVIKVARTGSIGDGKIFILPVEDAVRI
                     RTGESGEDVL"
     gene            1395..>3394
                     /locus_tag="VEC56_05825"
     CDS             1395..>3394
                     /locus_tag="VEC56_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13705.1"
                     /translation="MVTTEQYARGPLLRAVVEDVHEEMMRSLVDAPAPERAPKRPALE
                     ARLAACGFEESVAVATAFRGACVRMLANGASDVDAVEIVEACLRSASPDAAVRNLERY
                     LDNTSPSVFVRTMLAARPVLHLVTTVLGTSQHLADILIRQPGFVYWLMEQRTWESPDD
                     VASYEGWLERETEPFRTVEGKLNAVRRMHRQALLKIGAMDLVGIAPVEDVTRRLSDLA
                     DACARTVLEIVFDEIALDDTHPRGLAVIAMGKLGGRELNYSSDIDLIFASDDADEDTM
                     VFYTKVARRVTDAINALTEEGYLYRVDLRLRPDGKAGPLVNSEAALRVYYENRGRPWE
                     FQALLKARTIAGDAELGARVLETLAKLIFNPALPYSPLEAVGAMRDQIKDNLPSSQRG
                     YNIKLMAGGIRDIEFVAQAIQLMNGHRLPEIRTGNTLEALAHVRRLDLLKPWDVDNLA
                     AAYRFFRLVEHRLQMMHQFQTHTLPDSDAEVVLLARRVSTGPLGSYTTESFVEVLSKH
                     LNNVRTFADSFFAGESVHPHSVLLMLPEDHERAVSVLQHYGIRDVPRAMRTLHAMAYG
                     SFPRFLDRRARASFENLLPLLLEDLAATGDPDQTLVHVSQIAAAERSETAFYKMLTDS
                     TPARKLIVAVAGMSSLLTRELCAQIGTLDSLLEHGDRVDETLT"
BASE COUNT          563 a         1162 c         1108 g          561 t
ORIGIN      
        1 cgcggtgcgc gccaccatcg caccattgtc ggtgcaaaac tccaatagcg gcgcgtggta
       61 ggcaatccct gccgcgtcgg cggtcgcgtg catcttcgca cgcagcgggc ggttcgcggc
      121 gacgccgccg gccaggtaca cgtggcggac gccgagagag cgcgcgcact ggatcgtgcg
      181 ttccaccagc acgtcgacga tggccgcctg cgcggacgcg gcgatgttcg caaccatctc
      241 cggcgtcaat tcaccgcgcg cgtcgacgta ctgccgcacc gccgtcttga ggccggagaa
      301 cgagaagtcg aagccggcgc ggtcgagcat cgcgcgcgga aagtcgatcg cggttgcgtc
      361 gcccgcttcg gcggcgcgct gaatggcggg gccgcccggg aatcccagac ccaagagctt
      421 ggcgatcttg tcgaaggcct cgccggccgc gtcgtcgcgc gtgccgccga cgagttcgta
      481 gcgcccgatt cgttcgacgc gatacagcga cgtgtgcccg cccgacacca ccagcaccac
      541 gcacggacac tgcagcggtt cgccgccgag ttcgttcgag agcacgtgtg cttcgatgtg
      601 gtgcacgccg accaccggcc tctgcaatcc cagcgcaagc gacttcgcga agcccacgcc
      661 gaccagcagc gagcccacca gaccggggcc gttgctcacg cccacgccgc ccaagtcgtt
      721 cgcgcccacc ttcgcgcgcg cgagcaccgc gtcgtacacg gaaagcagcg tgcgcatgtg
      781 ctggcgcgac gcgatctcgg gcacgacgcc gccgaagtcc tcgtgcacga gctggctcgc
      841 ggtgacgtgg gagagcaacc cgcgctcgcg cgagtagagc gcgagcgacg tgtcgtcgca
      901 ggaggtttcg attccgagaa cgatcatggt gttcgtgacg cgcgcggcat tccgcgccga
      961 ggggagcaat gtctagagaa cgtcttcgcc gctctcgccg gtgcggatgc gcacggcgtc
     1021 ttcgaccggg agaatgaaga tcttgccgtc gccgatcgag ccggtgcgcg cgaccttgat
     1081 cacggtctcc accactttct cgacgtcggc gtcggccacg aagatctcga gcttgctctt
     1141 gggcagaaaa tcgacgcggt attcgctccc gcgatacagc tccgtgtgcc ccttctggcg
     1201 cccgaacccc ttgacctcgg agacggtcag cccctggacg ccgatctcca tcagcgccga
     1261 cttgacgtcg tcgagcttga agggcttgat gaatgcttcg atcttcttca tgaacgaccg
     1321 tcggtagttt acggtggtcg gggaacggac gcaagtgcta tgttgatcgc gtcttatcca
     1381 gtcccgaagc cgttatggtg accaccgagc aatacgcacg cggacccctg cttcgcgcgg
     1441 tcgtcgagga cgtccacgag gagatgatgc gctcgctcgt ggacgcgccc gcgcccgagc
     1501 gcgcgcccaa acgtcccgcg ctcgaagcgc gcctggccgc gtgcggcttc gaggaatcgg
     1561 tggccgtcgc gaccgcgttt cgcggcgcgt gcgtgcgcat gctcgccaac ggcgccagcg
     1621 acgtggacgc ggtcgagatc gtcgaagcct gtctgcgcag tgcctctccc gacgcggccg
     1681 tccgcaacct cgagcgctac ctcgataaca ccagcccgtc cgtgttcgtg cgcaccatgc
     1741 tggcggcacg gcccgtgctc catctcgtca ccaccgtgct cggcaccagc cagcacctcg
     1801 ccgacattct gattcgacag cccggcttcg tgtactggct catggagcag cgcacgtggg
     1861 agtcgcccga cgacgtcgcg agctacgaag ggtggctcga gcgcgagacc gaaccgttcc
     1921 gcaccgtgga gggaaagctc aacgcggtgc ggcgtatgca ccggcaggcg ctcttgaaga
     1981 tcggcgccat ggacctggtc ggtatcgcgc ccgtcgagga cgtgacgcgc cgtttgagtg
     2041 atctcgccga tgcgtgcgcg cgcacggtcc tggagatcgt gttcgacgag atcgcgctgg
     2101 acgacaccca cccgcgcggc ttggccgtca tcgcgatggg gaaactcggc ggccgcgaac
     2161 tcaattacag cagcgacatc gacctcatct tcgcgagcga cgacgccgac gaagacacga
     2221 tggtgttcta caccaaggtc gcgcggcgcg tgaccgacgc catcaacgcg ctgaccgaag
     2281 agggctacct ctatcgcgtc gatttgcgct tgcggccgga cggaaaggcg ggcccgctgg
     2341 tcaactcgga ggcggcgctg cgcgtgtatt acgaaaaccg cgggcggccg tgggagtttc
     2401 aggcgctctt gaaggcgcgc acgatcgcgg gcgacgccga gttgggcgcg cgcgtgctgg
     2461 aaacgctggc gaagctcatc ttcaatcccg cgctgcccta ctcgccgctc gaagcggtgg
     2521 gtgcgatgcg cgatcaaatc aaagacaacc tgcccagctc gcagcgcggc tacaacatca
     2581 agctgatggc gggcgggatt cgcgacatcg agttcgtggc ccaagcgatt caactcatga
     2641 acggccatcg cctccctgag attcgcaccg gcaacacgct ggaggcgctc gcccacgttc
     2701 gccggctcga cttgctgaag ccgtgggacg tcgacaacct cgccgccgcg tatcgcttct
     2761 ttcggttggt cgagcaccgg ctgcagatga tgcaccagtt ccagacccat accctccccg
     2821 attccgacgc ggaggtggtg ctgctcgcgc ggcgcgtgtc gacggggccg ctcgggagct
     2881 acaccaccga gtcgttcgtc gaagtgctgt cgaagcacct caataacgtg cgcacgttcg
     2941 cggatagttt tttcgcgggc gaatcggtgc acccgcactc ggtgctgctc atgctgcccg
     3001 aggaccacga gcgcgcggtc agcgtcttgc agcactacgg catccgcgac gtcccgcgcg
     3061 cgatgcgcac gctgcacgcg atggcgtacg gatcgtttcc gcgcttcctc gaccggcggg
     3121 cgcgcgcgtc gttcgagaac ctgttgccgc tcctgctcga ggacctcgcc gccaccgggg
     3181 acccggatca gacgctggtg cacgtgtcgc agatcgccgc cgccgagcgc agcgagaccg
     3241 ccttctacaa gatgctcacc gactcgacgc ccgcgcgaaa gctgatcgtc gccgtggccg
     3301 ggatgtcgtc tttgttgacg cgcgagttgt gcgcgcagat cgggaccctc gactcgctgc
     3361 tcgagcacgg cgaccgcgtc gacgagacac taac
//
LOCUS       DASPGK010000210         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1342176, whole genome shotgun sequence.
ACCESSION   DASPGK010000210 DASPGK010000000
VERSION     DASPGK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342176"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VEC56_05830"
     CDS             complement(<1..699)
                     /locus_tag="VEC56_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HXV13706.1"
                     /translation="MMPAAKSFDPVMGVDVHIIQPPGPVPPRPIPHPFIGMLIDPFDF
                     VPIIGATVYVNGSPRALAGTAGKALPPHIPIGGMFVPPPPGNECEMFMGSSTVIADGD
                     PFSYTALPALSCQSIGMPAPFRTNPKKKTKMKSLVLPTSVVLPIPMGPPVLVGGSPTI
                     SMMAIGMRVGMAGLGAAFKKLRKMQKASRKMKAVSDRIQAAAAKAMKKLGVPPSAQNK
                     VRRAICSVTGHPVDV"
     gene            complement(696..1964)
                     /locus_tag="VEC56_05835"
     CDS             complement(696..1964)
                     /locus_tag="VEC56_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13707.1"
                     /translation="MEKLRALWNEFASNPDARVLRWLVRTDEWDIVRVFLEVERSETG
                     ECPELFLVFDRPFASMTRYGIDLAQDMRKQFADSQEELSAQGLDQPWRPPAVTGVADS
                     RVLVAHCESLCGHYEDIDYLALVLLPSQVAGYSQWDGWLQKLVGGEIPENVRVLVCDD
                     VTSPQLDALAAKEPKRVVTQKLDLNMPSLIAELVKEHRGAGGPGADFREQFVALTTAA
                     GKGDIAAARKAADASIATARANKWPLQEVTALMAFANACLAAGDAKQAVKCYRDARKV
                     TATLQGDEAAVGAKVDVQTRLAEGAAMVSAGAFVEASAVYEATAPIAEKQGDPLMTIE
                     SWRMAAYCHEQARNPESAWKCGQAALKVGEKLEPDARKSTTLGYAAAGLMRVAKKKPY
                     KDQAPVLERKIGKLLGPGWENGIEKGASKS"
BASE COUNT          330 a          729 c          694 g          402 t
ORIGIN      
        1 gacgtcgacg ggatgccctg taacgctgca gattgctcga cgcaccttgt tctgagcgct
       61 aggagggact cctagcttct tcatcgcctt ggcggcagct gcttgtatcc gatcggacac
      121 cgccttcatc tttcggctgg ccttctgcat cttccgcagc ttcttgaacg ccgcccccaa
      181 gcccgccatc cccactcgca ttccgatcgc catcatcgaa atcgtcggcg aaccgccgac
      241 cagcaccggc ggtcccatgg gaatcgggag cacgacgctc gtcgggagga ccagcgactt
      301 catcttggtc ttcttcttgg gattcgttcg gaacggagcg ggcatgccga tgctctggca
      361 gctcaacgcg ggaagcgcgg tgtaactgaa cgggtcaccg tcggcgatga cggtcgaact
      421 tcccatgaac atctcgcact cgttgccggg ggggggcggg acgaacattc cgccgatcgg
      481 aatgtgcgga ggcaacgcct tgccggcggt gccggccagc gcgcggggac tcccgttgac
      541 gtaaacggtc gccccgatga tgggcacgaa gtcgaaagga tcgatgagca tcccgatgaa
      601 gggatgcggg atgggacgcg gcgggacggg cccgggcggc tggatgatgt ggacgtcgac
      661 gcccatcacc gggtcgaagg attttgcggc cggcatcatg acttgctagc acccttctcg
      721 atgccgttct cccagccggg ccccaagagc ttcccgatct tccgctccag cacgggggcc
      781 tggtccttgt aaggcttctt cttcgcgacc cgcatcagtc ccgcggccgc gtagccgagc
      841 gtcgtgctct tgcgcgcgtc gggttctagt ttctccccga ccttcagtgc ggcctgcccg
      901 cacttccacg cactctccgg attcctcgcc tgctcgtgac agtacgcggc catgcgccag
      961 ctctcgatgg tcatcaacgg gtcgccttgc ttctccgcaa tcggagcagt cgcttcgtag
     1021 accgcggacg cttccacgaa ggcgccggcg ctcaccatgg ccgctccctc ggcgaggcgg
     1081 gtttgcacgt ccaccttggc acccaccgcg gcttcgtcgc cctgcagcgt cgcggttacc
     1141 ttgcgcgcgt cgcggtagca cttgacggcc tgcttggcgt cacccgcggc gaggcacgcg
     1201 ttggcgaacg ccatcagcgc ggtcacttcc tgcagcggcc acttgttcgc gcgcgcggtc
     1261 gcgatggacg cgtcggctgc cttgcgcgcg gcggcgatgt cgcccttgcc ggccgcggtc
     1321 gtcagtgcga cgaactgttc gcgaaaatcc gccccgggac ccccggcgcc gcggtgctcc
     1381 ttgaccagct cggcgatgag cgagggcatg ttcaggtcga gcttctgggt cacgacacgc
     1441 ttgggctcct tggccgcgag cgcgtcgagc tgcggcgagg tcacgtcgtc gcacacgagg
     1501 acgcgcacgt tctccgggat ctcccccccg acgagtttct ggagccagcc gtcccactgc
     1561 gagtacccgg cgacctggct cgggagaaga acgagggcga ggtagtcgat gtcctcgtag
     1621 tgaccgcaga gcgactcgca gtgcgccacc aaaacgcgcg agtcggcgac acccgtgacc
     1681 gccggcggcc gccacggctg gtcgaggcct tgcgcggaaa gctcctcctg cgaatccgcg
     1741 aactgcttgc gcatgtcttg cgcaaggtcg atcccgtagc gcgtcatgga cgcgaacggc
     1801 cggtcgaaca ccaggaagag ctccgggcac tcgccggtct cgcttcgctc cacctcgagg
     1861 aacacgcgca cgatgtccca ctcgtccgtc ctgacgagcc agcgtaggac gcgggcgtcg
     1921 gggttggacg cgaactcgtt ccagagcgcg cggagtttct ccacgcgacg gacgacggcg
     1981 tttcgtacgg gtgggttggt cacggtctac cgatcaagca ggtcccgaag aaccgcctgg
     2041 cttgacagcg tttctactcg ggacctacag tgcgcgcatg cgcctacgaa cacgtgctct
     2101 gatcttcgtt cttgtcttgg tcgcagtcgt ggcggcggtt tggatcgccg acgac
//
LOCUS       DASPGK010000211         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1459211, whole genome shotgun sequence.
ACCESSION   DASPGK010000211 DASPGK010000000
VERSION     DASPGK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459211"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1233
                     /locus_tag="VEC56_05840"
     CDS             352..1233
                     /locus_tag="VEC56_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13708.1"
                     /translation="MSAAKPDRAARGVGPGRVIVVPFVLFVVVAAIGIPYYVLPHAER
                     LRHALHPWFRPSGYVGQTAGIITFAGFLFMWLFPLRKKLRKQEWLGPVPRWLDVHIGV
                     GLMLPIVGAIHAGFRFGGVIGIGYIAMLIVCLSGFVGRYLYVRIPRGRSGVELSLAEV
                     SAQQRALLAELADATGLSVLDVQEILNVESSPARVGIMAAFAQMARDDMARGRALRKL
                     RRRLKSKGATNKKRLEDAMTLARREMSLHQQARLLDATHSIFRHWHAAHRPVAITAFI
                     AVTIHVVVVVAVGATWF"
     gene            1247..2674
                     /locus_tag="VEC56_05845"
     CDS             1247..2674
                     /locus_tag="VEC56_05845"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXV13709.1"
                     /translation="MMMLITTIAFIVFTLLLMRGHMKSRHGDAKSAPCPRCRKKVPHG
                     AARCPSCGVPMQLYEVVRAGAVSAEESGGKGSVQPHAVVRADLCVGCAACVPVCPEPG
                     AIRMVGKLATVNKDLCKGHGKCVEACPVGGIVLATGASVHRVEVPLVNAAFESNIPGV
                     YVVGELGGRGLIKNAINEAAVAMEHVASERTRKHAAVPDDVLDVVIVGSGPAGLSAGL
                     EALRRKLRYVVLEQGALADTIRKYPRAKFLLAEPLTVPLYGNLWVADGSKERLLEVWE
                     RIIASTGLEVRTHHRVTGITRENGWLRVDTPEHAFRARHVILAMGRRGSPRRLGVPGE
                     ELGKVIYDVVDMESFAGQRMLVVGGGDSAVESAVGLAKQKGTTVVLCYRGDAFARVKE
                     RNRVNLEQAVHCGTIRVALRSQVREVRPDSVVLDVGGREEILPNDVVVVRIGGDPPYK
                     LLEEIGVRIVKKDVPLPKEEEPVVA"
     gene            2667..>2942
                     /locus_tag="VEC56_05850"
     CDS             2667..>2942
                     /locus_tag="VEC56_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13710.1"
                     /translation="MRSAATTRRVMVATLIASSCLGSSARAQVSPGPLAKVHADLDGN
                     LGCVKCHGKGEGAMDQKCLACHGEIAATIAKRTGFHGHEARSNCAHCH"
BASE COUNT          465 a          959 c         1019 g          499 t
ORIGIN      
        1 cgatgcccgc gagcatggga ccggccggtt cctgcgcatg acgcatccca catttcgaac
       61 tacgtaatcc ttgccacaga cgcgcgcgat agccattatg gcaacgtgct cgatatcgcc
      121 gccccgcatc cacgacgcgc acaaatagaa tagaaaagct cttctattct tccccgtcgt
      181 ggagcacgtc tctctcgaag ggcgtgtcgt tccgccgtcc gcgtattgcg gccgcggcac
      241 gggacttgcg ccattctcac agtgacgcgc acacacgtaa atcttggtta attcggttct
      301 gggcgagcag tgagaatgag caccccgggt atggcagttc cggtggcgag agtgtccgca
      361 gcgaagcccg atcgcgccgc gcggggcgtg ggccccgggc gcgtgatcgt ggttcccttc
      421 gtcttgttcg tcgtcgtcgc ggcgatcggt atcccgtact acgtgcttcc tcacgccgag
      481 cgcctgcgcc acgcgctgca cccctggttt cgaccctccg gctacgtcgg ccagacggcc
      541 ggcatcatca cgtttgccgg tttcctgttc atgtggctgt ttccgctgcg caagaagctg
      601 cgcaagcagg agtggctcgg ccccgtcccg cgttggctcg acgtccacat cggcgtgggg
      661 ctgatgctcc cgatcgtcgg cgcgatccac gccgggttcc gcttcggcgg cgtcatcggc
      721 atcgggtaca tcgcgatgct catcgtgtgc ttgagcggat tcgtggggcg ttacctctac
      781 gtgcgcattc cccgcgggcg ttcgggcgtc gaattgagcc tggccgaagt ctcggcgcag
      841 caacgcgcgc tcctggccga gctcgccgac gcgaccggtc tctccgtgct cgacgtgcaa
      901 gagatcctca acgtggagtc gtccccggcg cgcgtgggaa tcatggcggc cttcgcgcag
      961 atggcgcgcg acgacatggc gcgcggccgg gcgctgcgca aactccggcg ccgcttgaag
     1021 agcaagggtg cgaccaacaa gaagcgcctc gaggacgcga tgacgctcgc gcgccgcgag
     1081 atgtcgttgc accagcaggc gcgcctgctg gacgcgacgc attcgatctt tcggcattgg
     1141 cacgcggccc atcgcccggt cgccatcacc gccttcatcg cggtcacgat ccacgtggtc
     1201 gtggtcgtcg cggtgggcgc gacctggttc tagcgggtga ctcaccatga tgatgctcat
     1261 caccacgatt gcgttcatcg tcttcacgct gctcctgatg cgcgggcaca tgaagagccg
     1321 gcacggcgac gccaagtccg cgccgtgtcc gcgctgccgc aagaaggtgc cgcacggggc
     1381 cgcgcgctgt ccgagctgcg gcgtgcccat gcaactctac gaagtcgtgc gcgccggagc
     1441 cgtgtccgcc gaggaaagcg gcggcaaggg aagcgtgcaa ccgcacgcgg tggtgcgcgc
     1501 ggacctgtgc gtgggctgcg ccgcgtgcgt tccggtgtgc ccggagccgg gtgcgatccg
     1561 tatggttggg aagctcgcga cggtcaacaa ggatctgtgt aagggccacg gcaagtgtgt
     1621 cgaagcctgc ccggtgggcg gtatcgtgct cgcgaccggg gcgagcgtcc accgcgtcga
     1681 agtccccctc gtcaacgccg cgttcgagag caacatcccg ggcgtgtacg tggtcggcga
     1741 gttgggcggt cgcggcctca tcaagaacgc gatcaacgaa gcagcggtcg cgatggagca
     1801 cgtggcaagc gagcgcacgc gcaagcacgc cgccgttccc gacgacgttc tcgacgtggt
     1861 gatcgtgggc agcggtccgg ccggattgag cgcggggctg gaggcgctgc gccgcaagct
     1921 ccgctatgtc gtgctcgagc agggcgccct cgccgatact attcgcaagt acccgcgcgc
     1981 gaagttcctg ctcgcggagc cgttgacggt tccgctctac ggcaacctgt gggtcgccga
     2041 cggttccaag gaacgcctgc tcgaggtgtg ggagcgcatc atcgcgagca ccgggctcga
     2101 ggtgcgcacg caccaccgcg tgaccggcat cacgcgcgag aacggatggc tccgcgtcga
     2161 cacgccggag cacgcgttcc gtgcgcgcca cgtcattctc gccatgggcc ggcggggaag
     2221 cccgcgccgc ctcggtgtcc ccggcgaaga gctcggcaag gtgatctacg acgtggtcga
     2281 catggagtcg ttcgcgggtc aacgcatgct ggtggtgggc ggcggcgaca gcgcggttga
     2341 gtcggcggtg gggctggcca agcagaaagg gacgacggtg gtcttgtgct accgcggcga
     2401 tgcctttgcg cgggtcaagg agcgcaatcg cgtcaacctg gagcaagcgg tccattgcgg
     2461 cactatccgt gtcgcgctga ggagccaggt gcgcgaggtt cgacccgatt ccgtcgtgct
     2521 cgacgtcggc ggccgcgagg agattctccc caacgacgtc gtcgtggttc gcatcggcgg
     2581 cgacccgccc tacaagctgc tcgaggaaat cggcgtgcgc atcgtgaaga aggacgtgcc
     2641 gctgcccaag gaagaggagc cggtcgttgc gtagcgccgc gacgaccaga cgcgtgatgg
     2701 tcgcgacact aatcgcctcg agttgtttgg ggtcgagtgc gcgcgcccag gtctcacccg
     2761 ggccgctcgc gaaggtccac gcggacctcg acggcaacct cgggtgcgtc aagtgccacg
     2821 ggaagggcga aggcgccatg gaccagaagt gcctcgcgtg ccacggagag atcgcggcca
     2881 cgatcgcgaa gcgcaccgga ttccacgggc acgaggcgcg ctcgaactgc gcgcactgcc
     2941 ac
//
LOCUS       DASPGK010000212         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_406470, whole genome shotgun sequence.
ACCESSION   DASPGK010000212 DASPGK010000000
VERSION     DASPGK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406470"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VEC56_05855"
     CDS             <1..286
                     /locus_tag="VEC56_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13711.1"
                     /translation="YHSLSNRPLKALEIMIKLDEPSLSDADKQRLWLRTAVCQRSMND
                     FAGAKDTLERLVESFPGRPEFERLARRNLEQLVGAQTDGVAILQKTTTLD"
     gene            440..1777
                     /locus_tag="VEC56_05860"
     CDS             440..1777
                     /locus_tag="VEC56_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13712.1"
                     /translation="MKICPFMSHMLGADGNVLEIESGAHGSSGNTGDVVVLGYDGDGG
                     VGVKSDRKAKSQAKPGRENPATSHLYCLRETCRFYRTDDGECTFDAIIAGVDKARKAA
                     EASERAVEKSGRKDDKKDDTSAAVARELDKFWKFQTKSVAEMISSFGEVEKRQIDTIT
                     KLKNEVEKKIASIVPSEEEEDERHETMNAIKDGIAEIKDTIDARNDGFETLTTTVSDL
                     VLGFDDSIKQIKVQWEQIMNRIQALEAIAPRMQGVESNVGKAVENIDRLERNWAEMMD
                     LVRTDRDRKKDDSASQAKRREAMKFNNLGVTSYHHGDMKLARDQFLEALKRDDTFAEC
                     YNNLGLVYTELNENDRAAEAFTKAIKLNPELHAAYNNLGYVYYKQGNFGRAVEMYNEA
                     LGRSTNNGSAYTNLGNAHFKLGNKDEARKAWKKAIELDPSNDKAQKSLKSLDD"
     gene            complement(1877..2122)
                     /locus_tag="VEC56_05865"
     CDS             complement(1877..2122)
                     /locus_tag="VEC56_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HXV13713.1"
                     /translation="MRHTGKVKWFNENKGYGFILSDDGARDIFVHYSEIRDDGYRTLA
                     EGETVEYELMDSPKGPQAKSVQRNGKIAPAASPEAAL"
     gene            2557..3987
                     /locus_tag="VEC56_05870"
     CDS             2557..3987
                     /locus_tag="VEC56_05870"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV13714.1"
                     /translation="MRTTIPHAAVLGESGEVTKASLNELIRRALAASSPKPCALEIYD
                     SVTQRVLFLRDRQIYAAAESTGGQYSPTSIREFLVGVSQMSFPRACWYELNTKILHSL
                     LVVSQKKPALRVATNLVDLDELLDKIEAEGKSSIVAATREDFFALLRYEKGKAAALCM
                     ELSAATPREGSFREEFLVKVYTRSAEKPLTISLYEDFLVSYAPDAKNLPESFAGRFED
                     VFLSKPPVVALRFKDREIGRWEVDRPRLRIGRTPDNDIVIDNLAVSRLHALIEEEKGG
                     YFVRDCDSLNGTEVNGARVTRRKLEDGDEITIAKHTIQFRVQGGHAVAEPETMAGFDQ
                     TMIFQRSALNAGAPGAAPTPPPHPTPHAHDPSEGHDTRTATMLAVDASRPPRLVVHTE
                     FGDRIVEIEDDGVVIGKDQDADVHVAGMFVAGRHAEIIRENGRTILRRVGGLRPVRVG
                     GRAVKEIELKDNDEIQIASESFVFHE"
     gene            4013..>4139
                     /locus_tag="VEC56_05875"
     CDS             4013..>4139
                     /locus_tag="VEC56_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXV13715.1"
                     /translation="MRYVLMSDIHGNLEALQATIEAARRFEPYQLMCLGDVVGYGA"
BASE COUNT          845 a         1390 c         1277 g          627 t
ORIGIN      
        1 gtaccactcg ctctccaacc gcccgttgaa ggcgctcgaa atcatgatca agctcgacga
       61 gccctcgcta tccgacgccg ataagcagcg cttgtggctg cgcaccgcgg tgtgccagcg
      121 ctcgatgaac gattttgccg gcgccaagga cacgctcgaa cgcctggtag agtcgttccc
      181 ggggcggccc gagttcgagc ggctggcgcg gcgcaacctc gagcagctgg tcggcgcgca
      241 aaccgacggt gtcgccatcc tccagaaaac cacgacgctc gactgatccg gcccgtccgt
      301 tggcccgtct ccatccgctc cccggcacgg actatgctag gaatccgcgg atactacgag
      361 aatcatcgag accgtcgtgg ccgctgcgcc cgacgagata cccgaccatc atcgtaagga
      421 tcgctgaagg agcgagatca tgaaaatctg cccgttcatg tcgcacatgc tgggcgccga
      481 cggcaacgta ctcgagatcg agtccggcgc gcacggctcg tccggcaaca ccggcgacgt
      541 cgtcgtgctc ggctacgacg gcgacggcgg cgtcggcgtc aagtctgacc gcaaggcgaa
      601 gtcgcaagcg aagccggggc gcgagaaccc ggccacgtcg cacttgtact gcctgcggga
      661 gacgtgtcgc ttctatcgca ccgacgacgg cgagtgcacc ttcgatgcga tcattgccgg
      721 cgtcgacaag gcgcgcaagg ccgccgaggc cagcgagcgg gcggtcgaga agtcgggtcg
      781 taaggacgac aagaaggacg acacgtcggc cgcggtcgcg cgcgagctcg acaagttttg
      841 gaagttccag accaagagtg tcgccgagat gatttcgagc ttcggtgaag tcgagaagcg
      901 acagatcgac accatcacga agctcaagaa cgaggtcgag aagaagatcg cgtccatcgt
      961 tcccagcgag gaggaggaag acgagcgtca cgagaccatg aacgcgatca aggacggcat
     1021 cgccgagatc aaggacacca tcgacgcgcg caacgacggc ttcgagaccc tgaccacgac
     1081 cgtgtccgac ttggtgctcg gattcgacga cagcatcaag cagatcaagg tgcagtggga
     1141 gcagatcatg aaccgcatcc aggcgctgga agccatcgcg ccgcgcatgc agggtgtcga
     1201 gtccaacgtc ggcaaggcgg tcgagaacat cgaccggctc gagcgcaact gggccgagat
     1261 gatggacctg gtgcgcaccg accgcgaccg caagaaggac gactccgcgt cgcaggccaa
     1321 gcgccgcgag gcgatgaagt tcaacaacct cggggtcacc agctaccacc acggcgacat
     1381 gaagctggcg cgcgaccagt tcctggaagc gctcaagcgc gacgacacct tcgcggagtg
     1441 ctacaacaac ctcgggctcg tctacaccga gctcaacgag aacgatcgcg ccgccgaggc
     1501 gttcaccaag gccatcaagc tcaacccgga gctgcacgcc gcgtacaaca acctgggcta
     1561 cgtgtactac aagcagggga acttcgggcg cgcggtggag atgtacaacg aggcgctggg
     1621 ccgcagcacc aacaacggct ccgcgtacac caacctgggc aacgcgcact tcaaattggg
     1681 taacaaagac gaggctcgca aggcgtggaa gaaggcgatc gagctggatc catccaacga
     1741 caaggcgcag aagagcctga agagcctcga cgactaacca gccccatcga gggcctcagc
     1801 aaaaagacgc gatccccgca cccgcggctg cgcaggtacg gggatccgcg ttttttcgag
     1861 gacaacgaaa acgagcctag agagccgcct ccggcgacgc agccggcgca atcttcccgt
     1921 tgcgctgaac cgacttcgcc tgggggccct tcgggctgtc catcagctcg tactccaccg
     1981 tctcgccttc ggcgagcgtg cgatacccgt cgtcacgaat ctccgaataa tgcacgaaga
     2041 tgtcgcgcgc gccgtcgtcg ctgaggatga acccgtaacc tttgttttcg ttgaaccact
     2101 tcactttacc agtatggcgc aattccgaat tcccccttcc taaaagcaag aacccaccgc
     2161 gtgccgatcg cacccgatgg atcccctact gctcgattat tgactcgctg agaaacacgt
     2221 ggccaacctt gcctgcgaac gcggaggatt ttaccacggt ccgcttgcgc gtgtcaaggc
     2281 gctatacggc gcccgcacgc cccaatcccc ctcccgcgaa acgacgagaa atcatgagca
     2341 gcggcgcgcg cgcgtttcga gcgcaccgaa cgccgattgc attgcgcgat ttcagcgccg
     2401 ttcgagccgt ccgcgccagg ccggtttgcc gcgtcccgac gccccgtttc gcgccatcgt
     2461 cgcccgctcg gtgcccccat cggtggcatg gaacttgccc tttctcgttc gccgacaatg
     2521 cctttgcgaa ggtcgctggg cgagaaaggt cgcaacgtga gaaccaccat tccacacgcc
     2581 gcagtactcg gagagagcgg cgaggtcacc aaggcctcgc tcaacgagct catccgtcgt
     2641 gcgctcgccg ccagctcacc caagccgtgc gcgctggaga tctacgacag cgtaacccag
     2701 cgcgtgctgt tcttgcgcga ccggcaaatc tacgccgccg ccgaatcgac cgggggccag
     2761 tattcgccca cgtcgatccg cgagttcctg gtcggggtct cgcagatgag cttcccgcgc
     2821 gcctgctggt acgagctcaa caccaagatc ctgcactcgc tgctcgtggt ctcgcagaag
     2881 aagcccgcgc tgcgcgtggc gaccaacctg gtcgatctgg acgaactgct ggacaagatc
     2941 gaggcggagg gtaagagttc gatcgtcgcc gccacccgcg aggacttctt cgcgctgctt
     3001 cgctacgaga aaggcaaggc cgcggcgctg tgcatggagc tctcggcggc caccccgcgc
     3061 gagggctctt tccgcgaaga gttcctggtc aaagtctaca cccgcagcgc cgagaagccg
     3121 ctcaccatca gcttgtacga agacttcctg gtctcctacg cgcccgacgc caagaacctg
     3181 cccgagtcgt tcgccgggcg cttcgaggac gtgttcctgt ccaagccgcc cgtggtcgcg
     3241 ctgcgcttca aggaccgcga gatcggacgc tgggaagtgg atcggccccg tctgcgcatc
     3301 ggccgcaccc ccgacaacga catcgtgatc gacaacctgg ccgtgtcgcg actgcacgcg
     3361 ctcatcgaag aggaaaaggg cggctacttc gttcgcgact gcgacagcct gaacggcacc
     3421 gaggtcaacg gcgcgcgtgt cacgcgtcgc aagctcgagg acggcgacga gatcaccatc
     3481 gccaagcaca cgatccagtt ccgcgtgcag ggcggtcacg cggtggccga gccggagacc
     3541 atggccggct tcgaccaaac catgatcttc cagcgcagcg cgctcaacgc cggtgcgccg
     3601 ggcgcggctc ccacgccccc accgcacccg acaccgcacg cgcacgatcc gtcggagggt
     3661 cacgacacgc gtaccgcgac catgttggcg gtggacgcca gccgcccgcc ccggctggtc
     3721 gtgcacaccg agttcggcga ccgcatcgtc gagatcgaag acgacggcgt ggtcatcggc
     3781 aaggaccagg acgccgacgt gcacgtggcc ggcatgttcg tcgcgggccg ccacgccgag
     3841 atcatccgcg agaacggtcg caccatcctg cgccgcgtgg gcgggcttcg cccggtgcgc
     3901 gtgggaggcc gggccgtgaa ggagatcgag ctcaaggaca acgacgagat ccagatcgcc
     3961 agcgagtcgt tcgtgttcca cgaataggcc acgccaaccc gaagaggctg cgatgcgcta
     4021 cgtcttgatg tccgacatcc acggcaacct cgaagccctg caggcgacga tcgaagccgc
     4081 gcgccgtttc gaaccctacc agctcatgtg cctgggcgac gtggtgggtt acggcgccg
//
LOCUS       DASPGK010000213         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_406519, whole genome shotgun sequence.
ACCESSION   DASPGK010000213 DASPGK010000000
VERSION     DASPGK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406519"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2008
                     /locus_tag="VEC56_05880"
     CDS             185..2008
                     /locus_tag="VEC56_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV13716.1"
                     /translation="MVRHGFALILVTAMLANAQPSGAADSAKKPVSKSVTAAGNTQFL
                     MFGMQPAGNYEVRQNTTLDRSVVASPLGSVDMDDVTGVGDTYVFTLTGVEPVTPGTPT
                     GFAAAGSTNGCATLSWNAPPTAEYVTDYTLLWRRTGTVFTDSAQVSLTDIVQAGSRWS
                     TTRCGFANGTYTFAIRAHNAFDLWSGRSNEASATVTNENTQGPPPPTGIAGTENPVGC
                     LRVSWTRVGDPTVTGYRMYFATRPRSQGAYTDSVDVSGTTSVASRCGLSAGTYFASVR
                     SFTGAGLMSIYSSEVSVTLQGPDVTPPVVSQRDPASGATNVARNTGIFFVVTDARSGV
                     DAASIVVRVNGIQETPVTTATTSGYAVSVDPAADLPANTAITVQVTAADRAPTPNVLT
                     TSWTFTTGASASNDVLAPTIAAVTPLANATGVSASGAIEVDISDSGLGVALGSVQLII
                     NGSNVAFTVQGTPASVRITHRPASPFTAGAQVSVRVEACDRASTSNCAVPFEYSFTVA
                     GSNLASGTGDIVPDGYWAGDPARPLEVRNLPRAWTVRIFDAAGFTVRRFENTGSDGAN
                     WTWDFANDGGQRVAPALYLVRVTDSSGSLQRAGRFLVQSGR"
     gene            2005..2853
                     /locus_tag="VEC56_05885"
     CDS             2005..2853
                     /locus_tag="VEC56_05885"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HXV13717.1"
                     /translation="MSRAAIASRRHLAATLSLALPFALAYAPVLVELVRQWADDPNYA
                     HGFFVVPMAAWLARRRRTRYHAAPSTPDATGFVLLSIGALLYLAGSAAAELFTARVSL
                     VLVLGGLLWAIEGRARARALAFPVVFLFCMVPLPYVVYYQLTFPLQIESSRMAAAILG
                     MAGMPVVREGNILSLEGYSLEVVTACSGLRSIMTLGTMAIFLTDFMRLSTWSKVLLAA
                     SALPVAVAANVARLATTAGIAAIGGAQAAESFLHELSGIVVFITGLVVLFVTGKVLQW
                     IERARR"
     gene            2829..3470
                     /locus_tag="VEC56_05890"
     CDS             2829..3470
                     /locus_tag="VEC56_05890"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HXV13718.1"
                     /translation="MDRTRTTIIAAAALSLMAVAGYGFRAGEPRGAASLGLSGVRVDE
                     RVYLDETMDEDFLEVLRAREVLFRTYDPDADEPVWLFLGYFDRQKEGSQVHSPRHCYP
                     GSGWNIEQELEAPIEWRDGVLRALVVNNGVERRLVCFWYQTPFGIEDDVFRLKMALTR
                     QALMRRPQDVVFASVSTPLDSGLEGAWERAAGVARDVEGEIARLYAALARERA"
     gene            3467..>3676
                     /locus_tag="VEC56_05895"
     CDS             3467..>3676
                     /locus_tag="VEC56_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13719.1"
                     /translation="MNLARAIDDYLDALSAARLALRSPSILAPFLLFGLLQCVIITML
                     AFFTSRPLAPFMPPVVGALGGEASLH"
BASE COUNT          538 a         1297 c         1214 g          627 t
ORIGIN      
        1 cgatattctg cttgaccagg acccctccat tcgccaaacg taccttcgcg agtgacgcgg
       61 cccggccgcc gtgcacggtt tttgcagccc ccgaccgtca cgatcctcat ccagtcttaa
      121 tcgcaaccgc acgtttcggt cgatccgagt cggcgtggaa gcaaaccgct tccgggagtt
      181 gataatggtc cgtcatgggt tcgcactcat tctcgtcacg gcgatgctcg cgaacgcaca
      241 gccgtccggg gccgcggata gcgccaagaa acccgtgtcg aagagcgtca cggcggcggg
      301 caatacgcaa ttcctgatgt tcggtatgca gccggcgggc aactacgagg tgcgccagaa
      361 taccaccctc gaccgcagcg tggtggcgag cccgctgggg agcgtcgaca tggacgacgt
      421 caccggggtc ggcgacacct acgtgttcac cctgaccggc gtcgagcccg tgacccccgg
      481 caccccgacc ggcttcgccg ccgccgggtc gaccaacggg tgcgcgaccc tgtcgtggaa
      541 cgcccccccg acggcggagt acgtgaccga ctacaccctg ctgtggcgtc gcaccggaac
      601 cgtgttcacc gactccgccc aagtgagttt gaccgacatc gtgcaggcgg ggagccgctg
      661 gagcaccacg cgctgcggct tcgccaacgg cacctatacc tttgcgatcc gagcccacaa
      721 tgccttcgac ctgtggagcg ggcgctccaa cgaagcgtcc gcgacggtca ccaacgagaa
      781 tacgcagggt cctccgccgc cgaccggcat cgccgggacc gagaacccgg tcgggtgtct
      841 gcgcgtcagt tggacgcggg tgggcgatcc gaccgttacc ggctaccgga tgtacttcgc
      901 gacgcgaccg cgcagtcagg gcgcctatac cgactcggtc gacgtgtccg gcacgacctc
      961 ggtcgcgtcg cggtgcggac tctccgccgg gacctacttc gcgtccgttc gctccttcac
     1021 cggggccggg ctcatgagca tctactcgag cgaggtctcg gtgacgctcc agggccccga
     1081 tgtgacccca ccggtcgtga gccagcgcga cccggccagc ggggccacca acgtggcgcg
     1141 caacaccggc atcttcttcg tcgtcaccga cgcccgcagc ggtgtcgacg cggcgagcat
     1201 cgtggtgcgc gtcaacggca tccaggagac gcccgtcacc acggccacca cgtcgggcta
     1261 cgcggtatcg gtcgaccccg ctgcggatct gccggccaac accgcgatca ccgtgcaggt
     1321 gacggcggcc gaccgcgcgc ccacgcccaa cgtgctcacc acgtcgtgga cgttcacgac
     1381 cggtgcgagt gcgagcaacg acgtgctcgc gcccaccatc gcggcggtga caccgctcgc
     1441 caacgcgacc ggtgtttccg ccagcggcgc catcgaggtc gacatctccg actcggggct
     1501 gggcgtagcg ctgggatcgg tccaactcat catcaacggg agcaacgtgg ccttcaccgt
     1561 gcagggaaca ccggccagcg tgcgcatcac gcatcgaccg gcgagtccgt tcacagccgg
     1621 ggcccaggtc tccgtgcggg tcgaagcgtg cgaccgtgcc tcgacctcca attgcgcggt
     1681 tccgttcgag tattccttca ccgtggcggg gagcaacctg gcctccggta cgggcgacat
     1741 cgttcccgac ggctactggg ccggcgaccc cgcgcgtccg ctcgaggtgc gaaacctccc
     1801 gcgcgcgtgg acggtgcgca tcttcgacgc ggccggcttc acggttcggc gcttcgagaa
     1861 caccggttcc gacggtgcca actggacctg ggacttcgcc aacgacgggg gacagagagt
     1921 cgcccccgcg ctctacttgg tgcgcgtcac cgattccagc ggcagcctgc agcgcgcggg
     1981 ccgcttcctg gtccagtcgg gtcgatgagc cgcgcggcga tcgcctctcg ccgccacctc
     2041 gccgcgaccc tttcgctcgc ccttccattc gcactcgcgt acgcgccggt tctcgtcgaa
     2101 ctggtgcgcc aatgggcgga tgaccccaac tacgcacacg gcttcttcgt cgtcccgatg
     2161 gccgcgtggc tcgcgcgacg acggcgcact cgctaccacg cggctccctc cacacccgac
     2221 gcgaccggct tcgtgctgtt gtcgatcgga gcgctcctgt acctcgcggg cagcgccgcc
     2281 gccgaactgt tcaccgcgcg tgtctcgctc gtgctggtcc tgggcggact cctgtgggcg
     2341 atcgaagggc gcgcgcgcgc gcgcgccctg gcgttcccgg tggtgttcct cttttgtatg
     2401 gtgccgctcc cgtacgtcgt ctactaccag ctcacgtttc cgctccaaat cgagagctcg
     2461 cgcatggcgg ccgcgatcct cgggatggcc gggatgccgg tggtgcgcga aggcaacatc
     2521 ctgagcctcg aggggtactc gctcgaagtg gtgacggcgt gcagcgggct gcgttcgatc
     2581 atgaccctgg ggacgatggc gatcttcctc acggacttca tgcgcctgtc gacgtggtcg
     2641 aaggtgctcc tggccgcctc cgcgctgccg gtggcggtgg cggccaacgt cgcgcgcctg
     2701 gcgaccacgg cgggtattgc cgccatcggc ggcgcgcagg ccgccgagag tttccttcac
     2761 gagctttccg gcatcgtggt attcatcacc gggctggtcg tcctcttcgt caccggtaag
     2821 gtgctgcaat ggatcgaacg cgcacgacga taatcgcggc cgccgcgctt tcgctcatgg
     2881 cggtggctgg gtacggcttc cgcgcgggcg agccccgcgg tgcggcgtcg ctgggactgt
     2941 ccggcgtgcg cgtggacgag cgtgtgtacc tcgacgagac catggacgag gacttcctcg
     3001 aggtgctgcg cgcgcgcgag gtcctctttc gcacctacga cccggatgcc gacgagccgg
     3061 tgtggctctt cctcggctac ttcgaccgtc agaaggaggg gtcgcaggtg cactcgccgc
     3121 gccactgcta cccgggctcg gggtggaaca tcgagcagga gttggaagcg ccgatagaat
     3181 ggcgcgacgg cgtgctgcgc gcgctggtcg tgaacaacgg agtggagagg cgcctggtgt
     3241 gcttctggta ccaaacaccg ttcgggatcg aggacgacgt gtttcgattg aagatggcac
     3301 tcacccggca ggccttgatg cggcgcccgc aggacgtcgt gttcgccagc gtgtcgacac
     3361 cgctcgactc cggactcgag ggcgcgtggg agcgcgcggc cggggtcgcg cgcgacgtcg
     3421 aaggtgagat cgcgcgcctg tacgccgcac tcgcacggga gcgcgcgtga atctggcccg
     3481 cgccatcgac gactacctcg acgcgctgtc cgccgcgcgc ctcgcgcttc gaagccccag
     3541 catactcgcg ccctttctcc tgttcgggct gctccagtgc gtgatcatca ccatgctcgc
     3601 gttcttcacg tcgcgtccgt tggcgccgtt catgccgccg gtcgtgggcg cgctcggcgg
     3661 tgaagcgtcc ttgcac
//
LOCUS       DASPGK010000214         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_291104, whole genome shotgun sequence.
ACCESSION   DASPGK010000214 DASPGK010000000
VERSION     DASPGK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291104"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..750)
                     /gene="pyrF"
                     /locus_tag="VEC56_05900"
     CDS             complement(43..750)
                     /gene="pyrF"
                     /locus_tag="VEC56_05900"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXV13720.1"
                     /translation="MDTPDRLIVAIDVDSLDAARALITTLSPLARWFKIGSELYTREG
                     PAAARLVKDAGAKLFLDLKFHDIPNTVQRSVRSALTLGADMMTLHASGGPAMLRAARN
                     AADDAKRDDVILVGVTVLTHFSESEFNAIFPTSRKVEETVLALSKVARAGGMDGVVAS
                     AQELPAIKNAMGHDFIVVTPGIRLPGAGKDDQTRVVTPQQAIKDGADFIVVGRPITAA
                     ADPLAACDEVLRQMGDA"
     gene            complement(788..1813)
                     /locus_tag="VEC56_05905"
     CDS             complement(788..1813)
                     /locus_tag="VEC56_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13721.1"
                     /translation="MRSRSGIAVLALACAIACGCAVAPTTPPGVLPTSLDAGSGVYGA
                     WIEVDYRPPHGKKVDVDGELLAVEDGRVYVLLGDVVRPVPIDSIQKARVTFYNPQTSA
                     VAGLVALGTMATISNGFYLVITAPLWLMIGSGAAASRSHEPILDVPKHGWSEIVAYAR
                     FPQGMPADFVPQPVTPVVETIVIETPPKQPVAIAPGDQSAWGFAFGMGTARYDEQSDV
                     AAVLGLNVSKKWGSLGVRFSVGNRDPVEGSSEDLAIDGEVFDLAFLVGVRTESRGVQM
                     ALRAGPAAWGLDLGDLEDVRSSFAAQGELFFYPSSTVGFGTIVAYNNNSLQDYYIVTL
                     GIAIGPR"
     gene            complement(1800..>2925)
                     /locus_tag="VEC56_05910"
     CDS             complement(1800..>2925)
                     /locus_tag="VEC56_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13722.1"
                     /translation="SCPTFYVAGHHRPLAEGFSSSIAPSLEATDVDQLDVGVVGAGRF
                     EVVMKNEAYETHVVRHVDVLALPHRADERILRGVDGTFWAVANVTPPSFASADEGECT
                     DALRAIDGVERFGPADSTNLAAKETVEIAFDDPPEGRCGIAVACRQSLLSTYLLYQTF
                     AYMGREAGHWIAELERGELGEAGSALVEMLGGIVVSIETAPGVWVDVGEIREFGPIGV
                     DQHLLAFDAPLDWTGHARMHMTRGAWRVDGVKLGTLVERVEPIRVPPSRVLKGDRADE
                     FALALLTDPTRSLTTLPGDHYTLIYQLPDRSAHYEVFLESRGYYIEWMREDWLREENP
                     AHLAQLFFDPASALARLAPEYKKVEAGMEETFWGSRYAKP"
BASE COUNT          523 a          990 c          916 g          496 t
ORIGIN      
        1 gcgcttgaag ttcatcgtcg gcttcgcgct gtaggtcgac gcttacgcgt cgcccatttg
       61 gcgaagcact tcgtcgcacg cggccagtgg atccgctgcc gcggtgatgg gacggcccac
      121 cacgatgaag tcggcgccgt ctttgatggc ctgctgcggg gtgacgacgc gcgtctggtc
      181 gtccttgccc gcacccggca agcggatgcc cggtgtgacc acgatgaagt cgtgccccat
      241 cgcgttcttg atcgcgggca actcctgcgc cgacgccacc acgccgtcca tcccccccgc
      301 gcgcgccacc ttcgagagcg ccagcaccgt ctcttcgacc ttgcgcgagg tgggaaagat
      361 cgcgttgaac tcgctctccg agaagtgcgt gagcaccgtg acgcccacca agatgacgtc
      421 gtctcgcttc gcgtcgtcgg ccgcattgcg cgccgcgcgc agcatggccg gtccgcccga
      481 ggcgtgcagc gtcatcatgt ccgcaccgag tgtcagcgca cttcgcaccg agcgctgcac
      541 cgtgttgggg atgtcgtgaa acttcaaatc gaggaagagc ttcgcacccg cgtccttgac
      601 caggcgcgcg gccgccgggc cctcgcgcgt atacaactcg gagccgatct tgaaccagcg
      661 cgcgagcggc gaaagtgttg tgatcaacgc tctcgccgcg tcgagcgagt cgacgtcgat
      721 ggcgacgatg aggcggtcgg gggtatccat agctcttctc gttaggcgat cgaatcgatc
      781 tcgcggctca ccgcggtccg atcgcgatcc caagcgtcac gatgtagtag tcctgcaaac
      841 tgttgttatt gtatgccacg atcgttccga atccaaccgt agacgatgga taaaagaaca
      901 actcgccttg tgccgcgaac gaggagcgga catcctcgag gtcgccgaga tcgagtcccc
      961 aagccgccgg accggcgcga agcgccatct gcacgccgcg cgactccgtg cgcacgccga
     1021 cgaggaacgc gaggtcgaac acctcgccgt cgatggccag gtcctcgctc gaaccctcga
     1081 cgggatcgcg gttccccacg ctgaatcgaa cgcccagact cccccatttc ttcgatacgt
     1141 tcagccccaa caccgccgcg acatcgctct gttcgtcgta gcgtgccgtg cccatgccga
     1201 aggcgaaacc ccacgccgat tgatcgcccg gcgcgatcgc aacaggctgc ttgggcggag
     1261 tctcgatcac gatggtctcg acgaccgggg tcaccggttg cggtacgaaa tcggccggca
     1321 tcccttgcgg gaagcgcgcg tacgcgacga tttcggacca cccgtgcttg ggcacgtcga
     1381 ggatcggctc gtgcgagcgc gaggcggctg caccgctgcc gatcataagc cacagcggcg
     1441 ccgtaatgac gagatagaac ccgttgctga tggtggccat cgtgcccaga gcgaccagcc
     1501 ccgcgaccgc gctggtctgc gggttgtaga acgtgacccg ggctttctgg atggaatcga
     1561 tcggtacggg gcgcacgacg tcgccgagga gcacgtacac gcggccgtcc tcgactgcca
     1621 gcagctcgcc gtcgacgtcg accttcttcc catgcggagg tcgatagtcg acctcgatcc
     1681 acgccccgta gacgccggac ccagcgtcca acgacgtggg aagtaccccg ggcggtgtcg
     1741 tcggtgcaac cgcacagcca cacgcgatcg cgcacgcgag cgcgagtacc gcgatcccgc
     1801 tacggcttcg catagcgact cccccaaaac gtctcttcca tgcccgcttc gaccttcttg
     1861 tactcgggtg ccagacgagc cagcgccgag gcgggatcga aaaacaattg cgcgaggtgc
     1921 gccggattct cttcgcgcag ccagtcctcg cgcatccact cgatgtagta gccgcggctc
     1981 tcgagaaaca cctcgtagtg cgcgcttcgg tcgggcagct ggtagatcag cgtatagtgg
     2041 tcgcccggaa gtgtcgtcag cgatcgcgtc ggatcggtga gcaacgctag cgcgaactca
     2101 tccgcgcggt caccctttag cacgcggctg ggcggtacgc ggatcggttc gacccgctcc
     2161 accaacgtcc cgagtttcac gccgtcgacg cgccacgcgc cccgtgtcat gtgcatgcga
     2221 gcgtgaccgg tccaatcgag gggcgcgtcg aacgcgagaa ggtgttgatc gacgccgatg
     2281 gggccgaact cgcgaatctc gcccacgtcg acccacacgc ccggcgccgt ttcaatcgac
     2341 accacgatgc cacccagcat ctcgaccaat gcacttccgg cctcgcccag ctcgccgcgc
     2401 tcgagctcag cgatccaatg gccggcctcc cggcccatat aggcgaacgt ctgatagagc
     2461 agatacgtcg agaggagcga ctgacgacat gccacggcga ttccgcaacg gccctccgga
     2521 ggatcatcga atgcgatctc gaccgtttcc ttggctgcga ggttggtcga atcggccggg
     2581 ccaaagcgct ccacgccgtc gatggcgcga agcgcgtcgg tgcattcgcc ttcgtcggcc
     2641 gaggcgaacg acggcggtgt aacgtttgcg accgcccaga aggttccatc gacgccgcgt
     2701 aggatgcgct cgtcggcgcg atgcggcaac gcaagcacgt cgacgtggcg cacgacgtgc
     2761 gtctcgtagg cttcgttctt catgacgact tcgaaacgac cggcaccgac gacgcccacg
     2821 tcgagctggt cgacgtcggt ggcttcgagg gaaggtgcga tgctggaaga gaaaccctct
     2881 gccagcggac gatggtgacc cgcgacgtag aacgtcgggc aggac
//
LOCUS       DASPGK010000215        10229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_291219, whole genome shotgun sequence.
ACCESSION   DASPGK010000215 DASPGK010000000
VERSION     DASPGK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291219"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..512
                     /locus_tag="VEC56_05915"
     CDS             66..512
                     /locus_tag="VEC56_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13723.1"
                     /translation="MRKHPAVSALLVGSAAMAVAVASGCDRASTTDPDPPTGGQSYVL
                     DFDVFATTINPILSAQGCDNLTCHGGGIRGTFELSPNGNKNNQFDFEQARMQVVGSDP
                     PASPLVMKPLAPECGGPGHAGGYVFYQMDDPDYLAILAWIEAGEYR"
     gene            509..1195
                     /locus_tag="VEC56_05920"
     CDS             509..1195
                     /locus_tag="VEC56_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV13724.1"
                     /translation="MSARAAWILALASSALFASSAVSASEPDSLSTPAPQMPETTAVA
                     PEPARADASEPSDGLIYLVPSMKDAGFRVTNDRERFQHRIWFSPGYGQLGSQDLFAFR
                     VGYSPNTWLGYEVSLGHNPASSLHGLLHTFNVVVRYPLSGRFQPYATLGYGMMTVYPG
                     QAINADPVTKNALTAGGGLEIYIRNDVAIRGEMRGATVLGQQLNVEGTVAYDYLEYTI
                     GFAFFRSLGS"
     gene            1266..3254
                     /locus_tag="VEC56_05925"
     CDS             1266..3254
                     /locus_tag="VEC56_05925"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13725.1"
                     /translation="MRFATNRRTFASSFPTLVGALCVLVLSGFGCRDSAPDDVIVEEE
                     TTAAIVFVKTVGEETLNRSWAEGNLYKLSPISPDGIVTPITNFTGASISDPCVSFDGT
                     KILFSMRPPSGGNRNIYEINADGTGLRQVTSGGGHDFDPLYLPDDRILFTSSRAREMD
                     EYNHAPVENMYVVDADGSNLQRVSFNMSDDFDPTLNAEGNIVYTRWEHFGTVNRFPLF
                     VANPDGTAPFHKYGPHNRNFFHAQPTPDGRLVAIESTMIEEDAGPIAVLKLEAGPADP
                     TQGSTENFWNVLTPQVNTDGEPWPYGAFKYPSSLGGNTYVVSYTLPAALESDVDYGLY
                     TFTLNQTGAGTGGSPATISIDDLTFLYNDPNFNEYDAQLLAPRARPPVIPSTVDLNRT
                     DGVILANTVFNRALNDGQEVPIQGATGVDAIDSIAVVVGIPTAQGEPNDFSANEFEKR
                     ALLGFAPVQPDGSFRIRVPANTPISWATLDGLNRGFVVKRTWIYMRPGEEITKCAGCH
                     QDRMLNQTFNPNPNPIAATLPPTDLNIPPSGYRFINYRDHIGPLVAANCVSCHQPTYT
                     ADVPPDTIPPAGDLDLTELPDTTMEDQVFPRGYINLSGESEMGPRNVTVPAFPRRSPV
                     IDYLLGVDEGVGKNHDTLLNASEKELFNLWVMLGAQYR"
     gene            3277..3855
                     /locus_tag="VEC56_05930"
     CDS             3277..3855
                     /locus_tag="VEC56_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV13726.1"
                     /translation="MTTRRLMQAAAVALAIAGMTGGRAAAGADVGATLANHELASFDG
                     GKTKLASMRGDVVVVNFWATWCAPCRKELALFDGWNAEWKGRGARVVAISIDKDARKA
                     RRFAEEMGLQLTVVHDGPDGLARSLDLPSVPCTYLLDRDGNVVSVVRTSSHDELAVLK
                     GKVDSMVAGRKTPPQAAGMGSSATMPFNGDSR"
     gene            3852..4061
                     /locus_tag="VEC56_05935"
     CDS             3852..4061
                     /locus_tag="VEC56_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HXV13727.1"
                     /translation="MKCAYRIVLVLALGLTAACSSVRPYEKEHLADPIMTSSKDEAMR
                     ELKWLEAREGSTGGAGGAGGGCACK"
     gene            4091..5203
                     /locus_tag="VEC56_05940"
     CDS             4091..5203
                     /locus_tag="VEC56_05940"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HXV13728.1"
                     /translation="MTAVAGVQVAAWAPCVRAGIDETTASYLFNYYTDVEGVDVFGHY
                     VTTGLRFQNNLGLNFQWVHDRVVIPAIEAPPGTQEAADAITSASRPIASSADPYQDYI
                     KTRNSFEGGVSYHGYNAGYYVSKESDYFAQMISLGYNQGLRDDNLNIAVGASYSWDSI
                     EPLADQDTPGIPDYRRTTYWSVVATQVVTKTTVLRVGAEFNQVSGLQHDPYRNVYVAG
                     SNVPESHPDQRTRRDLFLGVSQYVYNRSSIKLDYRFYSDDWGVESHMVGLKLNQYVNE
                     EFVFRYRYRYYAQLPSEFYRVEYTDAGGIDGYQTGDYRLGDFGAHLFGGQVLWHPHRF
                     FGGLGTHAQVTFGYERYFNSNNFSADVFETGLQIAF"
     gene            5229..6875
                     /locus_tag="VEC56_05945"
     CDS             5229..6875
                     /locus_tag="VEC56_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13729.1"
                     /translation="MSRTALMISILVLGAVQAAAETFQPAPYLAQAQVDVLNKQVANE
                     QFGRDLFGEPYATVVVGNVDVYDRFPYLEARYFQVVSDPAWGRLLLGEIGQGLAAFDG
                     AASSFGRLKTPRGLSTDGRGLIYVADSGNDRVLVFRTVSEFDRIRLEPVYAIDDLNDP
                     YGVAHSDGGTPFDAGDDVLYVANTGKNEVRRYTLGTSGATLTDAIGALGSGDAHFAGP
                     TAITVGRRDGVANDDVYVSDAHNRRLVHLRDTGSSLSWVGAASHGLGLITSLDADHFG
                     NVYAASPQAGRVTKFTSALSPVASFTANTNRPRSFHVVFANVTDHRSGRQTRAGQGTG
                     VLVEEWGGSNGIRLLNLGVDMTDAAAVENENAVRVTLTDHANVSAEIVDPSNGQVVAR
                     HDAGVLAAGTRTIRFTDADNVAAWDAGAYRVTIRARTTYDNGSTSTVEVPIELSGSGG
                     PAMPQRLALLGNTPNPFNPTTTIRFAVPAGPSQPYRLRVYDVRGAFVRELAAGQIGGG
                     THEARWDGRDHRGTPVSSGVYLYRLEVGAAKLTGKMALVK"
     gene            6890..7495
                     /locus_tag="VEC56_05950"
     CDS             6890..7495
                     /locus_tag="VEC56_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13730.1"
                     /translation="MTTRLQGHGAGGRRPRWVVVLPAVGALVLGSCLGEPEVDERWTL
                     LEMVSVAPQPNQARSASQPLNVNVSGRVTYRDIVTGFMVAEVRYSPTLTAVSLPLDPD
                     EHNEESARSVDRILANSVTAGRATRAVTGWDHLVQSVNLSFTAQVPPAVLTSGGLFLV
                     LYMGSGDEIELANGDDSLVVTPFVSSDKEILHTGFPITIVP"
     gene            7492..8160
                     /locus_tag="VEC56_05955"
     CDS             7492..8160
                     /locus_tag="VEC56_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13731.1"
                     /translation="MSRAATRFGAGALALVVCAQCATAARADDTDSGWRIGVGAMLGG
                     ISFDSHLDDYRWDVTPTLQTGAQATVYHGRFATGARLWLAHTTQASGIPGETQAPRVN
                     LTGLEWIGQGRMVSYRGLELWGMVHGGRLFLAYDPDEMTFDPGAGAPITVAFDSISEW
                     DYGFGIEIRGAVTAQLALALQAEHTSFSLDTAHRNGDEIVETRERFDQWSLRVQAAWL
                     WNLD"
     gene            8195..9055
                     /locus_tag="VEC56_05960"
     CDS             8195..9055
                     /locus_tag="VEC56_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HXV13732.1"
                     /translation="MERNTRTWGGVCLLALSLLAGTPAGGGAQTESPAPANEFELNAP
                     HVLTEKKVRLSKDGNNVVRSGPGNTFAIVGVYSKGSEFLVIAKRDEWYNVRLSDTNTG
                     WIHSSLCQEFDDMSDLEFRPNPKLFSRVGSFALTGYTGGYSFDRKSNSLVFGGHIGYY
                     LFEFIEVDGTVGYTHIVRPAEIVESLFGLTLEEEDFHMLFYAMNLTWKVLPGRQLVPF
                     VTGGAGSSIMEGETETTFNWGVGLDFFVKKQTAVRFEFRNYMFESGVSSQARQDFQNF
                     EFGVGITYLL"
     gene            9067..9615
                     /locus_tag="VEC56_05965"
     CDS             9067..9615
                     /locus_tag="VEC56_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13733.1"
                     /translation="MRRFLLIAAALATLAVAARAGDPAPYSARAGLEVARDAAVSWAA
                     DAQLVYLENDEMVGPDGRAMRWGYLFYSPVKAKARGYSVRDGKILEASDLNFDFEPVP
                     LAENWIDSHAARVAAEQKAGQKYCQEFEGHLAAMLLIRGAFHEKKPDATTWAVVYESK
                     SQPMLFVIVDASNGDVVRTWRG"
     gene            9620..>10229
                     /locus_tag="VEC56_05970"
     CDS             9620..>10229
                     /locus_tag="VEC56_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13734.1"
                     /translation="MRARIILLACLVCAGLAPRATAQAAADTTGVVESIEVKRAETKE
                     PKHTSLKFLRDNRVFIRAQLDKLRVQVTRTRTDEAQTLDQRYLLLKQMSAAIAAARDT
                     VSAESLLVAQRQLMTSITQLGELEVQLSTIEDILAEHRTRLLGIEQDFLGHQETALVI
                     LVRGFAGKSAPATIVLQDQEDVVRVDLTPEQRASLEQGGIAQV"
BASE COUNT         1825 a         3434 c         3294 g         1676 t
ORIGIN      
        1 ttggccgaaa agcgactcct ccccggagga aagaaggaac aagcgacgtg gacgcacgag
       61 gaatcatgag gaagcatccc gcggtctcgg cactgctagt cggatcggcg gccatggctg
      121 tcgccgtcgc cagtggttgc gaccgcgcgt cgacgacgga tcccgatccg ccgacgggcg
      181 ggcagtccta tgtcctcgac ttcgacgtgt tcgcgaccac gatcaatccc atcttgtccg
      241 cgcaagggtg cgacaacctg acgtgccacg gcggcggaat ccgcgggacc ttcgagctct
      301 cgcccaacgg aaacaagaac aaccagttcg acttcgagca ggcgcgcatg caagtcgtcg
      361 gcagtgaccc acccgcgagc ccgctcgtca tgaaaccgct ggcacccgag tgcggcggcc
      421 ccggccacgc cggcggctac gtcttctatc agatggacga tcccgactac ctggccatcc
      481 tggcctggat cgaagcgggt gagtaccgat gagcgcgcgc gctgcgtgga tcctcgcgct
      541 ggcgtcgagc gcgctcttcg cgtcgagcgc ggtgtcggca agcgagcccg actcgctctc
      601 gacccccgcg ccacaaatgc cggagacgac ggcggtcgcg ccggaacccg cgcgcgcgga
      661 cgcgagcgag ccgagcgatg gcctgatcta tctcgtgccg tcgatgaaag acgcgggctt
      721 ccgcgtcacc aacgaccgcg agcgtttcca gcaccgcatc tggttcagcc ccggttacgg
      781 gcagctggga tcgcaggact tgttcgcgtt tcgcgtgggc tacagcccca acacgtggct
      841 cggctacgag gtgtcgctgg gtcacaaccc ggcctcgtcg ctgcacgggc tcttgcacac
      901 cttcaacgtg gtggtgcgct atcccctgtc gggacgcttc cagccctacg ccaccctcgg
      961 ctacggaatg atgacggtgt atccgggcca ggccatcaac gccgacccgg tgaccaagaa
     1021 cgcgctgacc gccggcggcg gtctcgagat ctacatacga aacgacgtcg ccatccgcgg
     1081 cgagatgcgc ggggcaaccg tgctcggaca gcaactcaac gtcgagggca ccgtcgctta
     1141 cgactacctg gagtacacga tcggcttcgc attctttagg agtctgggca gttagttaga
     1201 gtttccggcc gtgagcaccg tccgaatggg cgggaggagc ggcgggaggc gccaaggaga
     1261 gcatcatgag attcgcaacc aacagaagga ccttcgcctc gagtttcccg accctcgtcg
     1321 gtgcgctgtg cgtgctggtg ctgtcgggat tcgggtgtcg cgacagcgct cccgacgacg
     1381 tcatcgtcga ggaggagacc accgcggcga tcgtcttcgt caagaccgtg ggcgaagaaa
     1441 cgctcaaccg gagctgggcc gagggcaacc tctacaagct ctcgcccatc tcgccggacg
     1501 ggatcgtcac gcccatcacc aacttcacgg gcgcgtcgat cagcgacccg tgcgtgtcgt
     1561 tcgacgggac gaagatcctc ttctcgatgc gcccgccttc cggcggaaac cgcaacatct
     1621 acgagatcaa cgccgacggt accgggctgc gccaggtgac gagcggcggc gggcacgact
     1681 tcgacccgct gtatctcccc gacgatcgca tcctgttcac gagctcgcgc gcgcgtgaga
     1741 tggacgagta caaccacgcg ccggtcgaga acatgtacgt cgtcgacgcc gatggctcga
     1801 acctgcagcg ggtgagcttc aacatgagcg acgacttcga ccccacgctc aacgcggagg
     1861 ggaacatcgt ctacacgcgc tgggagcact tcggaaccgt caaccggttc ccgctcttcg
     1921 tcgcgaaccc ggatggtacg gcgccatttc ataaatacgg cccgcacaac cgcaacttct
     1981 tccatgccca gccgactccc gacggccgcc tggtcgccat cgagtcgact atgatcgaag
     2041 aggacgcggg cccgatcgcg gtcctgaagc tcgaagcggg tccggccgac ccgacccagg
     2101 gcagcaccga gaacttctgg aacgtgctga caccgcaggt caacaccgac ggtgagccgt
     2161 ggccgtacgg cgccttcaag tacccgtcgt cgctgggcgg caacacgtac gtggtgtcgt
     2221 acacgctgcc ggcggcactc gagtcggacg tcgactacgg tctctatacg ttcacgctga
     2281 atcagaccgg cgccggcacc ggcggcagcc cggccaccat ctcgatcgac gacctgacgt
     2341 tcttgtacaa cgatcccaat ttcaacgaat acgacgcgca gctgttggcg ccgcgcgcgc
     2401 gtccgccggt cattccgtcg acggtcgatt tgaatcgcac cgacggcgtc atcttggcca
     2461 acacggtgtt caaccgcgcg ctcaacgacg gccaggaggt ccccatccag ggtgccaccg
     2521 gcgtggacgc gatcgactcg atcgcggtgg tggtcggcat cccgaccgcg cagggcgagc
     2581 ccaacgattt ctcggccaac gagttcgaga agcgcgcgct cctgggcttc gcacccgtgc
     2641 agcccgacgg ttcgttccgt atccgcgtac cggccaacac gcccatcagc tgggccaccc
     2701 tggacggact gaaccgcggg ttcgtggtca aacgcacatg gatctacatg cgcccaggcg
     2761 aagagattac gaagtgcgcg ggctgccacc aggaccgcat gttgaaccag accttcaacc
     2821 cgaacccgaa cccgatcgcg gccacattgc cgccgacgga tctcaacatt ccgccctcgg
     2881 gctaccggtt catcaattat cgcgaccaca tcggaccgct ggtcgcagcg aactgcgttt
     2941 cgtgccacca gccgacgtac acggccgacg ttccgcccga cacgattccg cccgcgggcg
     3001 acctcgacct cacggaactg cccgacacga ccatggagga ccaggtcttc ccgcgcggct
     3061 atatcaatct ctcgggcgag tccgagatgg gtccgcgcaa cgtgaccgtg cccgcgttcc
     3121 cgcgccgctc gccggtgatc gactacctct tgggagtcga cgaaggcgtg ggcaagaacc
     3181 acgacacgct attgaatgcc tccgaaaagg agctgttcaa cctgtgggtc atgctgggcg
     3241 cgcagtatcg ctaggcccat cgaaggagcg aacgacgtga cgacacggag actcatgcaa
     3301 gcggcggctg ttgcgctcgc gatcgccggg atgaccggcg gtcgcgccgc ggccggggcg
     3361 gacgtggggg ccacactggc caaccacgag ctcgcctcat tcgacggcgg caagaccaaa
     3421 ctggcgtcga tgcgcggtga cgtcgtggtc gtcaacttct gggccacgtg gtgcgcgccg
     3481 tgccgcaagg aactggcgct gttcgacggg tggaacgcgg agtggaaggg gcgcggcgcg
     3541 cgtgtggtgg caatctcgat cgacaaggac gcccgcaagg cgcgtcgctt cgccgaggag
     3601 atggggttgc agctcaccgt cgtgcacgac ggacccgacg gcctggctcg ctcgctcgac
     3661 ctgccttcgg tgccgtgcac ctatttgttg gatcgcgacg gcaacgtggt gagcgtggtg
     3721 cgtacgagct cgcacgacga gttggcggtg ctcaagggca aggtcgactc gatggtggcc
     3781 ggacgcaaga ccccgccgca agcggcgggc atgggctcgt cggcgacgat gcccttcaac
     3841 ggagactcgc gatgaaatgc gcgtatcgaa tcgtgttggt tcttgctctc gggttgacgg
     3901 cggcttgctc gtcggtgcgc ccgtacgaga aggagcacct tgccgatccc atcatgacgt
     3961 cgtccaagga cgaagcaatg cgagagctca agtggctcga ggcccgcgag ggctcgactg
     4021 ggggcgcggg aggcgccggc ggcggatgcg cgtgcaagta acccataggc gtgcaacccc
     4081 gtggttgctg gtgacggccg tggcgggcgt ccaggtcgcg gcatgggcgc cgtgcgtgcg
     4141 cgccggcatc gacgaaacca ccgcgtccta cctgttcaac tactacacgg acgtcgaagg
     4201 cgtcgacgtg ttcggccatt acgtgaccac gggcctgcgg ttccagaaca acctggggtt
     4261 gaacttccag tgggttcacg accgggtcgt gatccccgcc atcgaggcgc ccccgggcac
     4321 gcaagaagca gccgacgcga tcacgtcggc cagccggccc atcgcgagca gcgccgaccc
     4381 gtaccaggac tacatcaaga cgcgcaactc gttcgagggc ggggtcagct accacggata
     4441 caacgcgggc tactacgtct cgaaagagag cgactacttc gcgcagatga tatcgctcgg
     4501 ctacaaccag ggcctgcgcg acgacaatct caacatcgcg gtcggcgcca gctacagctg
     4561 ggacagcatc gaaccgctcg cggatcaaga cacgcccggt attcccgact atcgccgtac
     4621 gacgtactgg agcgtcgtcg cgacccaggt cgtcaccaag accaccgtgc tgcgcgtggg
     4681 cgcggagttc aaccaagtga gcgggctcca gcacgatccc tatcgcaacg tgtacgtggc
     4741 cgggagcaac gttcccgaga gtcatcccga ccagcgtacg cgccgggacc tgttcctggg
     4801 cgtgagccag tacgtctaca accgctcgag tatcaagctc gactaccggt tctatagtga
     4861 cgactggggt gtggagtcgc acatggtcgg attgaagctc aatcagtacg tgaacgagga
     4921 gttcgtgttt cggtatcgct atcggtacta cgcgcagctg ccgtcggagt tctatcgcgt
     4981 cgagtacacc gacgcgggcg gcatcgacgg ctaccagacc ggcgactatc gcctgggcga
     5041 cttcggcgcc cacctcttcg gtggccaggt gctgtggcac ccgcaccggt tcttcggcgg
     5101 tctgggtacg catgcgcaag tgactttcgg gtacgagaga tatttcaaca gcaacaattt
     5161 ctctgcggat gtgttcgaaa ccggtctgca aatcgcgttc taaagcggac caaacaaagg
     5221 agccgggtat gagccggaca gcattgatga tcagcatcct ggttctgggc gcggtgcaag
     5281 ccgccgccga gacgttccaa cccgcaccct atctggcgca agcccaggtg gacgtgctca
     5341 acaagcaagt cgccaacgag caattcgggc gcgacctgtt cggcgaaccc tacgccaccg
     5401 tggtagtcgg aaacgtggac gtgtacgacc gcttcccgta tctggaagcg cgttacttcc
     5461 aagttgtctc cgacccggcg tggggccggc tcctgctcgg cgagatcggc cagggcctgg
     5521 ccgcgttcga cggcgccgcg agcagcttcg gacgcctgaa gaccccgcgc ggcctgagca
     5581 ccgacggccg cggattgatc tacgtcgccg acagcggcaa cgaccgcgtg ctcgtgttcc
     5641 gcaccgtgag cgagttcgac cgcatccggc tggaaccggt gtacgcgatc gacgacctca
     5701 acgatcccta cggcgtggcg cattcggacg gcggaacgcc gttcgacgcg ggcgacgacg
     5761 tgctctacgt cgccaacacc ggcaagaacg aagtccgtcg ttacacactc ggcaccagcg
     5821 gtgccacgct gaccgacgcg atcggtgcgc tcggcagcgg cgacgcgcac ttcgcgggtc
     5881 cgaccgccat cacggtcggc cgccgcgacg gtgtggccaa cgacgacgtc tacgtttccg
     5941 acgcccacaa ccgtcgtctg gtccacctgc gcgacaccgg ctcgtcgctt tcgtgggtgg
     6001 gtgcggcgtc gcacggcctg ggcctgatca cctcgctcga cgccgaccac ttcggcaacg
     6061 tgtacgcggc ctcgccgcag gcggggcggg tcaccaagtt cacctcggcg ctgtcaccgg
     6121 ttgccagttt tactgccaat accaaccgac cgcgcagctt ccacgtggtg ttcgcgaacg
     6181 tcaccgacca tcgctcgggc cgccagaccc gcgccggaca gggcacgggt gtactggtcg
     6241 aagagtgggg cggcagcaac ggcattcgct tgctcaacct cggcgtcgac atgacggacg
     6301 cggcggcggt cgaaaacgag aacgcggtgc gcgtcacgtt gaccgaccac gccaacgtga
     6361 gcgccgagat cgtcgacccg tccaacggtc aagtggtcgc gcgtcacgac gcgggtgtgc
     6421 tggcggcggg cacgcgcacc attcgtttca cggacgcgga caacgtggcc gcgtgggacg
     6481 cgggcgccta tcgcgtcacc atccgcgcgc gcacgaccta cgacaacgga tcgaccagca
     6541 ccgtcgaggt gccgatcgag ctgtcgggtt cgggcgggcc ggccatgccg cagcgcctgg
     6601 cgctcctggg caacacgccc aacccgttca acccgaccac gaccattcgc ttcgcggttc
     6661 cggccggccc cagccagccg taccgcctgc gcgtgtacga cgtgcgcggt gcgttcgtgc
     6721 gcgagctggc cgccggccag atcggcggcg gcacgcacga ggcgcgctgg gacggccgcg
     6781 accaccgcgg tacgcccgtg agctcgggtg tctatctcta ccgtctcgaa gtcggcgccg
     6841 ccaagctcac cggcaagatg gcgctcgtga agtaggcgag gaactctcga tgacgacgag
     6901 gctgcaaggt cacggtgcgg gtggacggcg accccgatgg gtcgtcgtcc tcccggccgt
     6961 gggggcgctc gtactcggga gctgcctggg cgagcccgaa gtcgacgagc gctggacgct
     7021 gctcgagatg gtcagcgtcg cgccgcagcc caaccaggcg cgctcggcca gccaaccgct
     7081 gaacgtgaac gtcagcgggc gggtcactta tcgcgacatc gtcaccggtt tcatggtggc
     7141 cgaggttcgt tacagtccca cactcaccgc cgtgagcttg cccctcgacc cggacgagca
     7201 caatgaagag tccgctcgct ccgtcgatcg tatactggcg aattccgtca ccgccggccg
     7261 cgccacgcgc gccgtcaccg gctgggacca cctggtccag agcgtcaacc tctccttcac
     7321 cgcccaggtt cctccggccg tgctcaccag cggcggcctg ttcctggtgc tgtacatggg
     7381 ctcgggcgac gagatcgagc tcgccaacgg cgacgactcg ctggtcgtga cgccgttcgt
     7441 ctcctccgac aaagagatcc tccacaccgg gttcccgatc acgatcgtgc catgagccgc
     7501 gcggcgacgc gtttcggtgc cggcgcgctc gcgctcgtgg tctgtgcgca gtgtgcgacc
     7561 gcggcacgcg ccgacgacac cgattcgggt tggcgcatcg gcgtgggcgc catgctcggc
     7621 ggcatctcgt tcgattccca cctcgacgat tatcgctggg acgtcacccc gaccctgcag
     7681 acgggcgcgc aggccaccgt gtaccacggg cgcttcgcca ccggcgcgcg tctctggctc
     7741 gcccacacga ctcaggccag cggcattccg ggcgagacgc aggcgccgcg cgtcaacctc
     7801 accggcctcg agtggatcgg acaagggcgc atggtttcct atcgcggcct ggagttgtgg
     7861 ggaatggtgc acggcggtcg attgttcctc gcctacgacc ccgacgagat gacgttcgac
     7921 ccgggcgcgg gcgcccctat cacggtcgcc ttcgattcca tctcggagtg ggattacggc
     7981 ttcggtatcg aaattcgcgg ggcggtgacg gcccaactgg cgctcgcctt gcaagcagaa
     8041 catacgagtt tctccctgga tacggcgcac cgcaatggcg acgaaatcgt cgaaacgcgc
     8101 gagcgtttcg accagtggag cctgcgcgtg caggcagcct ggttgtggaa tctcgactga
     8161 ccggttcgga accgaaccga ccgaaggaat cgccatggaa agaaacacac gcacgtgggg
     8221 cggggtttgt ctgctggcgt tgagcctctt ggcggggaca cccgccggcg gcggcgcgca
     8281 gaccgaatcg cccgcgcccg ccaacgagtt cgagctcaac gccccgcacg ttctcaccga
     8341 gaagaaggtc cgcctgtcga aggacggcaa caacgtcgtt cgcagcggcc ccggtaacac
     8401 cttcgccatc gtcggcgtct actcgaaggg ctccgagttc ctcgtcatcg ccaagcgcga
     8461 tgaatggtac aacgtgcgcc tgtccgacac caacacgggc tggatccact cgtcgttgtg
     8521 ccaggagttc gacgacatgt cggacctgga gtttcgcccc aacccgaagt tgttctcgcg
     8581 cgtcggcagc ttcgcgctca ccggatacac gggcggctac tcgttcgatc gcaagtccaa
     8641 ctcgctggtg ttcggcggcc acatcggtta ctacctcttc gagttcatcg aagtcgacgg
     8701 caccgtcggc tacacgcaca tcgtgcgtcc cgcggagatc gtcgaatcgc tcttcgggct
     8761 cacgctcgaa gaggaggact tccacatgct gttctacgcg atgaacctca cgtggaaggt
     8821 gctccccggg cgccagctgg ttccattcgt caccggcggc gcgggcagct cgatcatgga
     8881 gggcgaaacc gagaccacct tcaactgggg cgtcgggctg gacttcttcg tcaagaagca
     8941 gaccgcggtt cgcttcgagt ttcgcaacta catgttcgag tccggcgtgt cgtcgcaggc
     9001 gcgccaggat tttcagaact tcgaattcgg cgtcgggatc acgtacctgc tctaggagag
     9061 tcgtccatgc gtcgcttcct gttgatcgcg gccgcgctcg cgaccctggc cgtggcggcc
     9121 cgggccggcg acccggcccc ctactccgcg cgcgcgggcc tcgaggtggc gcgcgacgcg
     9181 gccgtttcgt gggccgcgga cgcgcagttg gtctatctcg agaacgacga aatggtgggg
     9241 cccgacggcc gcgccatgcg ctggggatac ctgttctact cgcccgtcaa ggccaaggcg
     9301 cgcggctact cggtgcgcga tggcaagatc ctggaggcct cggacctcaa cttcgatttt
     9361 gagcccgtac cgctggccga aaactggatc gacagccacg cggcgcgcgt ggccgccgag
     9421 cagaaggcgg gacagaagta ctgccaggag ttcgaggggc acttggccgc gatgctcctc
     9481 attcgcggcg cgttccacga gaagaagccc gatgcaacca cgtgggccgt cgtgtacgag
     9541 tccaaatcgc agccgatgtt gttcgtcatc gtcgacgcgt ccaacggcga cgtcgtgcga
     9601 acgtggaggg ggtagcgcca tgcgtgcacg catcatcctg ctcgcgtgcc tggtgtgcgc
     9661 cggcctcgcg ccgcgcgcga ccgcgcaagc cgccgcggat accaccggcg tcgtggagag
     9721 catcgaggtc aagcgcgccg agaccaagga acccaagcac accagcctca agttcctgcg
     9781 cgacaatcgc gtgttcatcc gcgcgcagct ggacaaactt cgggtgcaag tgacgcggac
     9841 gcgtaccgac gaggcgcaga ccctcgacca gcgctacctg ctgttgaagc agatgtcggc
     9901 cgccatcgcc gctgcgcgcg ataccgtgag cgcggagtcg ctgctcgtcg cacagcgcca
     9961 attgatgacc agcatcaccc aattgggaga actggaagtc cagctctcca ccatcgagga
    10021 tatcttggcc gagcatcgca cgcgattgct cggcatcgag caggacttcc tcggccacca
    10081 ggagaccgca ctcgtgattc tcgtgcgcgg cttcgccggc aagagcgcgc cggccaccat
    10141 cgtgctccag gatcaggaag acgtcgtgcg cgtcgacctg acgcccgaac agcgcgcctc
    10201 gctcgagcag ggcggcatcg cgcaggtgt
//
LOCUS       DASPGK010000216         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_524474, whole genome shotgun sequence.
ACCESSION   DASPGK010000216 DASPGK010000000
VERSION     DASPGK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524474"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1671)
                     /locus_tag="VEC56_05975"
     CDS             complement(118..1671)
                     /locus_tag="VEC56_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXV13735.1"
                     /translation="MTTTSAKIGTAMAWTMLSRVGRMVLGIVTSAIVVRGLGEHDYGV
                     LSVLRSVLMFVVLLAGVGTGQALLKYLPELRVARDRAGARALVRGVLVTHVGAWLALV
                     ALAYAVQPALEGVFHIDGVGLYVLIAVVLAVFEIVFMVATHVLNASFDAARLSLATLA
                     SHVVYAVALLVAFNRGWGVVGVLGAAAAGSAVATALVWSRVRVATDFDRGPEPATSGA
                     PVPAVTRSRVLRYSLPFAAVGVLNLVVWRQSETLLLAHFRSAEETGYFDAAYRLPQTV
                     LEFVPGTVWPLVMAGMSEAFARDRKSLRRAVSTYYRMLFALCAPICVAGAVAGGRFVE
                     VLYGPAMQPAAVPAQLFFVIFTVSFLSTPLSMALYVLEKTHVNLVIYLLLAVLNVGLD
                     IVLIPRFGVYGAIVPVAIAILLQPVLYYVAVHRFDTGVRIPFGYVGRCFLGAAPLFLM
                     APVLAVWDGAVGLLVAACAGAIACVIGYRWARVLGDEEIDSLSKLPVPGASAAAALLG
                     ATGSKRKGK"
     gene            complement(1668..>2753)
                     /locus_tag="VEC56_05980"
     CDS             complement(1668..>2753)
                     /locus_tag="VEC56_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV13736.1"
                     /translation="LGWEIAERFGFGRDEFVTRTPLGTVLGYSSAAIPSLLSGTTPAE
                     HGSWSMFKRAEGRGSFAFLERFPRLPHALEWRARRWVRRLADRRAGVRAYYDLYEIPL
                     HLLHRFDVGQKGDPFQPGGLSRETVFDWMQREGVVYRLWYYRTGEEDNLAAAEHALAG
                     EDEVLFVYTAELDALMHRVGIFHDSVGARLRRYRAFLSAMHSAARKRDRALTTVVLSD
                     HGMTDVTRVVDPWGALERSGRGVGRDFLAFFDSTMVRLWGDARAADVAVEAMGDGGRR
                     LCDDELRGMGCWFPAREYGDEIVLAQPGVLIVPSFMGSSAIAAMHGYHPEDAYSKGCF
                     MSDADVPAPASLLGFKSFLERVLRESR"
BASE COUNT          535 a         1020 c          855 g          343 t
ORIGIN      
        1 ccaccaccac ctcgtcgacg cacgacggga gctggcccat ggtgtagcga acgccgtcct
       61 cctcgttgta acacggaatg acgacgctga tcttgagtcc gcgatgcatg agacctccta
      121 cttccctttt cgtttcgatc cggtcgcgcc cagcagggcc gccgcggccg acgcccccgg
      181 gactgggagc ttggacaggc tgtcgatctc ttcgtcgccc agcacgcgcg cccagcggta
      241 gccgatcacg cacgcgatgg cgcccgcgca cgcggcaacc aaaagcccta ccgccccgtc
      301 ccacaccgcc aacaccggcg ccatcaggaa gagcggcgcg gccccgagga agcagcgccc
      361 gacgtacccg aacggaatgc gcacccccgt gtcgaagcga tgcaccgcga cgtagtagag
      421 caccggctgc aagagaatcg cgatggcgac cgggacgatc gcgccgtaca caccgaatct
      481 cggtatcagc acgatgtcca agccaacgtt gagaacggcg aggagcaagt agattaccaa
      541 attgacgtgc gtcttttcca gcacgtagag ggccatcgag agcggcgtcg acaagaacga
      601 cacggtgaag atgacgaaga agagctgcgc cggaacggcc gccggctgca tggccgggcc
      661 gtacaacacc tccacgaagc gtccgccggc gaccgcgccc gcaacgcaaa taggagcgca
      721 caacgcgaac agcatgcggt agtacgtgct caccgcgcgg cgcaggctct tacggtcgcg
      781 cgcgaaagcc tccgacatgc ccgccatcac cagtggccac accgtccccg gtacgaactc
      841 gagcaccgtc tgcggcagtc ggtaggccgc gtcgaagtac ccggtctcct ccgcgctgcg
      901 aaagtgcgcg agcaggagcg tctccgactg ccgccacacc accagattga gcacgcccac
      961 cgctgcgaac ggcagcgaat agcgcagcac gcgagaccgt gtcaccgccg gcaccggcgc
     1021 accactcgtt gcgggctccg gcccgcggtc gaagtccgtc gcgacgcgca cgcgactcca
     1081 aaccaatgcg gtcgccaccg cgctgccggc ggccgccgca cccagcacgc cgaccacgcc
     1141 ccagccacga ttgaaagcga ccagcagggc taccgcgtac acgacgtggc tcgcgagggt
     1201 cgcgagcgag aggcgggcgg cgtcgaaact cgcgttgagc acgtgcgtcg ccaccatgaa
     1261 cacgatctcg aacaccgcca gcacgacggc gatcagcacg tacaggccga ccccgtcgat
     1321 gtgaaacacg ccctccaggg cgggctgcac ggcgtatgcc agcgccacca gcgccagcca
     1381 tgcccccacg tgggtgacga gcacgccgcg aaccagcgcg cgcgcacccg cgcgatcacg
     1441 tgcgacccgc agctcgggca ggtacttgag aagcgcttgc cccgtcccca cgccggccag
     1501 cagcacgacg aacatgagca ccgatcgcag tacgctcagc acgccgtagt cgtgctcgcc
     1561 cagcccgcgc actacgatcg cgctggtgac gatgcccagc accatgcgtc ccacgcgcga
     1621 cagcatcgtc cacgccatcg cggtgccgat cttggccgag gtcgttgtca ccgcgactcc
     1681 cgcagcacgc gctcgaggaa cgacttgaag ccgagcaacg acgccggagc cggcacgtcg
     1741 gcgtcgctca tgaagcaccc cttggagtag gcatcctccg gatgataccc gtgcatcgcg
     1801 gcgatcgcgc tggatcccat gaagctcggc acgatcaaca cgccgggttg cgcgagcacg
     1861 atttcatccc cgtactcgcg ggccggaaac caacagccca tcccgcgcaa ctcgtcgtcg
     1921 cagagccgcc ggccgccgtc gcccatggct tccaccgcga cgtcggcggc ccgcgcgtcg
     1981 ccccacaggc gcaccatggt cgagtcgaaa aaggccaaga aatcgcgccc cacgcccctg
     2041 cccgagcgct ccagcgcgcc ccacggatcg actacgcgcg tcacgtcggt catgccgtgg
     2101 tcggacagca cgacggtggt gagcgcccgg tctcgcttgc gcgcggcact gtgcatcgcc
     2161 gaaagaaacg cccggtaacg gcgcagccgc gcccccaccg aatcgtggaa gatgccgacc
     2221 cgatgcatga gcgcatcgag ctccgcggtg tacacgaaca gaacctcgtc ctcgcccgcg
     2281 agcgcgtgct ccgccgcggc cagattgtcc tcctcgccgg tgcggtagta ccagaggcga
     2341 tagacaaccc cttcgcgctg catccagtcg aacacggtct cgcgcgaaag ccccccgggt
     2401 tggaacggat cgcccttctg ccccacgtcg aagcggtgca gaaggtggag tgggatctcg
     2461 tagaggtcgt agtacgcgcg cacgcccgcg cgtcggtcgg cgaggcgcct gacccagcgc
     2521 cgcgcgcgcc actcgagcgc gtgcggcaag cgcgggaagc gctcgaggaa tgcaaacgag
     2581 ccgcgcccct ccgcgcgctt gaacatcgac caggaaccgt gttcggccgg tgtggttccg
     2641 gagagcaagc tcggaatggc ggccgaactg tagccgagca cggtgcccag cggcgtgcgc
     2701 gtgacaaact cgtcgcgccc gaacccgaaa cgctcggcga tctcccagcc cag
//
LOCUS       DASPGK010000217         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_56367, whole genome shotgun sequence.
ACCESSION   DASPGK010000217 DASPGK010000000
VERSION     DASPGK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56367"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VEC56_05985"
     CDS             <1..944
                     /locus_tag="VEC56_05985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13737.1"
                     /translation="GSFVKDETEALRRAGVDLDVYFVNGRASKLAYAAMPFGFFARLR
                     RTRYDVVHVHHSFCGLVATRQRAVPVVWTFHEGEITGGTSDALREQPIKHVAYSKRMK
                     QFVARRVDALVVVAEHLRGPLGRPDAMYLPAGIDVDLFAPMETAEAQRRLGLSDQKRY
                     VLFPAVPSRVEKRYELARRAVEIARERAPELHDVELIALNNVPHEDVPLYMNASEVVL
                     LTSAFEASPVVIREALACNVPVLSTDVGDARVMLEGVAGCAIVPPDPERIADALRVVL
                     RSPRRVAGRGAMEQYSLANSAKALVALYQRVIEGHRR"
     gene            941..2152
                     /locus_tag="VEC56_05990"
     CDS             941..2152
                     /locus_tag="VEC56_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13738.1"
                     /translation="MNARRAVAIVRHSFYPFELNVKREAEALRDAGFDVHVICLRGPG
                     EAARETIEGVEVYRLPVTHRRGKILRYLWEYNAFFALASWKLARLHRKHRFAAVQVNT
                     MPDYLVFAAWYPKRAGARVVLHLHEPVPELFRTMFPKRWYTPFFVWTVTRVEQAAIRF
                     ADRVLTVTDQMRDNYVRRGADASKFTVVVNVPDDRLFRRERYADLRPRIDAIKAEERA
                     RGVFRVLTHGAIEERYGFDTVVRAIARARERVPGIQFRFMGAGEYLDEVKELARDLGV
                     SDRVHHLGFVPFEQMVEEILLADVCVVTVKRNAYSVLVHTNKMYEYMALGRPVIASRL
                     DSVAAYFPSDALSFFEPSDAAGLGEALGWAATHPQELAQHVAAADARYGALRWEKEKH
                     RYLAAYNLPQD"
     gene            2278..>3126
                     /locus_tag="VEC56_05995"
     CDS             2278..>3126
                     /locus_tag="VEC56_05995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HXV13739.1"
                     /translation="MAKAAKRAQTTKPRRATGKQGARATKRARAGEPETLPLFGEQAP
                     ESATKEKARSAPAAVVERGDSAAETPRRRRASDKASYSSAETIAQKQREISVSEFFAK
                     NRHMLGFDSPTRAILTAVKEAVDNSLDACEEAGLAPDILVEIHELAEDRYKLVVEDSG
                     PGIVKAQVPKIFGKLLYGSKFHELRQSRGQQGIGISAAVMYSQLTTGKSVKITSRVGA
                     KSKAHLYELQIDTKKNAPIVLDDGAVEWERPHGTRIEMELEAIYQKGKRSVDQYLHQV
                     ALANPHA"
BASE COUNT          527 a         1053 c         1043 g          503 t
ORIGIN      
        1 acggatcgtt cgtcaaggac gagaccgaag cgctccgtcg cgcgggtgtc gacctcgacg
       61 tctatttcgt caacgggcgc gccagcaagc tcgcgtacgc cgccatgccg ttcgggttct
      121 ttgcgcgctt gcggcgcacg cgctacgacg tcgtccacgt gcatcactcg ttttgcggtc
      181 tggtggcgac acgacagcgc gcggttccgg tggtgtggac gttccacgaa ggcgagatca
      241 cgggcggcac ctccgacgcg ctgcgcgagc aacccatcaa gcacgtggcc tactcgaagc
      301 gcatgaagca gttcgtcgcg cgacgcgtgg acgcgctggt ggtggtggcc gagcacctgc
      361 gcggaccgct cgggcgaccg gacgcgatgt acctgcccgc cggaatcgac gtcgacttgt
      421 ttgcgcccat ggagaccgcg gaggcccagc ggcgtctcgg cctctccgac cagaagcgct
      481 acgtcctgtt ccccgcggtg ccgtcgcggg tggagaagcg ctacgagctg gcgcggcgcg
      541 cggtcgagat cgcacgcgag cgcgcgcccg agctgcacga cgtggaattg atcgcgctca
      601 ataacgtccc gcacgaggac gtgccgctct acatgaacgc cagcgaggtg gtcctgctga
      661 cctccgcctt cgaggcctcg ccggtggtga ttcgcgaggc gctcgcatgc aacgtgcccg
      721 tcctctccac cgacgtgggc gacgcgcgcg tgatgctcga gggagtcgcg gggtgcgcga
      781 tcgttcctcc ggacccggag cgaatcgcgg atgcgctgcg ggtcgtgttg cgctcgcccc
      841 gtcgcgtcgc cggccgtggg gcgatggagc agtactcgct cgccaacagc gcaaaggcgc
      901 tggtcgcgct ctatcagcgc gtgatcgaag ggcatcgccg atgaacgccc gtcgcgcggt
      961 cgcgatcgta cgacactcgt tctatccctt cgagctcaac gtcaagcgcg aggcggaagc
     1021 cctacgcgac gccggcttcg acgtgcacgt gatctgcctg cgcggacccg gagaggcggc
     1081 gcgggagacg atcgaaggcg tcgaagtcta ccgcctgccc gtgacccatc gtcgcggcaa
     1141 gatcctgcgc tatctgtggg agtacaacgc cttcttcgcg ctggcgtcct ggaaactcgc
     1201 caggctgcat cggaagcatc gcttcgccgc cgtgcaagtc aacacgatgc cggactacct
     1261 ggtgttcgcc gcgtggtacc cgaagcgggc gggcgctcgc gtcgtcctcc atctccacga
     1321 gcccgtgccc gagctgttcc ggaccatgtt cccgaagcgg tggtacacgc cgttcttcgt
     1381 gtggacggtg acgcgcgtcg agcaggcggc catccgcttc gccgatcgcg tgctcaccgt
     1441 gaccgaccag atgcgcgaca actacgtgcg ccgcggcgcg gatgcgtcca agttcacggt
     1501 cgtggtcaac gtgcccgacg atcgcctgtt ccggcgcgag cgctacgcgg atctgcgccc
     1561 ccgcatcgac gcgatcaagg cggaggagcg cgcgcggggt gtgttccgcg tgctcacgca
     1621 cggtgccatc gaagagcgct acggcttcga caccgtcgtg cgcgcgattg cacgcgcgcg
     1681 agagcgcgtg cctggtatcc agttccgctt catgggcgcg ggggagtatc tcgacgaggt
     1741 gaaggagcta gcgcgcgacc tcggtgtctc cgatcgcgtc catcacctcg gcttcgtccc
     1801 cttcgagcag atggtcgagg agattctcct cgccgacgtg tgcgtcgtga cggttaagcg
     1861 taacgcgtac tcggtgctcg tacacaccaa caagatgtac gagtacatgg cgctcgggag
     1921 acccgtgatc gcgtcgcgat tggactcggt cgctgcgtac ttcccgtccg atgcactgtc
     1981 gttcttcgag cccagcgacg cggcgggtct cggggaggcg cttggctggg ccgcgacgca
     2041 tccccaagag ctcgcgcagc acgtcgcggc ggccgatgcg agatacggcg cgctgcgctg
     2101 ggagaaggaa aagcaccgct atcttgccgc gtacaatctc ccgcaggact gactcccctc
     2161 cgacccggcc ccgatttctt cttgtcgtgc ctcgcgatca ccgcctacca tccggatcga
     2221 attcggctcg gcgccgcgaa cgcggcgtcc gtatcgttcg aaccggaggt aagacgcgtg
     2281 gcgaaagccg ccaagagagc ccagacgacg aagccccgtc gcgcgaccgg caagcagggc
     2341 gcgcgcgcga ccaagcgcgc gcgcgccggg gaacccgaga cccttccgct gttcggcgaa
     2401 caggcgcccg agtccgccac gaaggaaaag gccaggtccg ctccggcggc cgtggtcgag
     2461 cgcggtgata gcgccgcgga gacgccgcgc cggcgccgcg cttccgacaa ggcgtcctat
     2521 tcgtccgccg agaccatcgc gcagaagcag cgcgagatct ccgtcagcga gttcttcgcc
     2581 aagaaccgcc acatgctggg cttcgacagc ccgacgcgcg cgattcttac ggccgtcaag
     2641 gaagccgtcg acaactcgct cgacgcgtgc gaagaggcgg gcttggcgcc cgacatcttg
     2701 gtcgagatcc acgagctcgc cgaggatcgc tacaagctgg tggtcgagga ttcaggcccc
     2761 ggcatcgtca aagcgcaagt gccgaagatc ttcggcaagc ttctgtacgg ctccaaattc
     2821 cacgagctgc ggcagtcgcg cgggcagcag ggcatcggca tctccgcggc cgtgatgtac
     2881 tcgcagctca cgaccggcaa gagcgtcaag atcacctcgc gcgtgggcgc gaagagcaag
     2941 gcgcacttgt acgagctgca aatcgacacc aagaagaacg cacccatcgt actcgacgac
     3001 ggcgccgtcg agtgggagcg cccgcacggg acgcgtatcg agatggagct cgaggccatc
     3061 tatcagaagg gaaagcgctc cgtcgatcag tatctgcacc aagtggcact cgcgaacccg
     3121 cacgcc
//
LOCUS       DASPGK010000218         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_56396, whole genome shotgun sequence.
ACCESSION   DASPGK010000218 DASPGK010000000
VERSION     DASPGK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56396"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEC56_06000"
     CDS             <1..180
                     /locus_tag="VEC56_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13740.1"
                     /translation="ALAAWTVIQGVTVAVHVAWLVVDPGYQPGLATTPLFAVTIAAAI
                     AALKRRRVADGNSGN"
     gene            complement(177..1538)
                     /locus_tag="VEC56_06005"
     CDS             complement(177..1538)
                     /locus_tag="VEC56_06005"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXV13741.1"
                     /translation="MQKRTTPLVAGIIAVMAFTGQEPVVRPVWDRIPVPAPETVGCLY
                     TEDGKMIMTDSYDGDGPAFVAAPVKPEPVRGEIGKNSTMYDELRALGVSAFEIDTMTR
                     ETKKVFDWRRVRKGQTFDLYTNEAGEIDSLILYTSPAEFVCVRRGIEYFTVAVEQVPF
                     EVSYHVTHGTIYDSIFASLQSQGVETDLAGSIDEIFGWTLDLAKDLRQGDQYALLYER
                     RTYETGYTALGTVLAARIVNLGKEYNAVRYTPESGTVGYYALDGKSMQKSMRRAPMKF
                     SRISSNYSSRRLHPVQRVYKPHYGVDYAAARGTPVYATGDGLVLAANYQSGNGNYVRI
                     HHNKTYETYYLHLSGFAKGVRPGMRVTQGQVIGYVGSTGLATGPHLCYRMKKGGRWVN
                     PRMLDLPAKEPVSPSEIVAFNAIRDAYLSRMYEALNEGLDRTTVVQAPARATSPGLTA
                     AMF"
     gene            complement(1839..4130)
                     /locus_tag="VEC56_06010"
     CDS             complement(1839..4130)
                     /locus_tag="VEC56_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXV13742.1"
                     /translation="MTPRHRSPLAVLLTAALALVPSLVRSEIVTVPFKGSAQIAEFEA
                     RGIEILAYTKYGVDVLAEGAALDYLRSRPYPISIHVVKPGQPHAVLDANLGQYHTYAE
                     TEALLTSLASTYPAIAQRSVIGLSLQGRNITALKISDNVALDEGEPEVLYMGNHHARE
                     LMSVEIPLLFAQYLLQNYGSNATVTEYVDSREIYVVPMVNPDGHYYVQLNHAGHWSTW
                     WRKNRRDNLDGFFGVDLNRNYGYQWGFDNIGSSPVTSSDVYRGTEEFSEPETQAIRNF
                     VNQREFTTWLSYHSYGELLLYGWGYIPANTPDHEAIAALADTLVVENGYLAGNPASGA
                     IYITNGASDDWGYGEQTSKPKIFAYTPEINSGAQGGFGPDETYILPTFNLLLPMNMKL
                     LRFADNPYRVVGPWAPEQNVVQAPFGNAVTRISWEPQDPQDPNPAASYTIEGCHEPST
                     FTDTATPAATGWTLGGFTYSLTGFSGGGYYSGNLNNLFSTITMAQPLVVDAAADTLRF
                     KVNYNVEVDYDYGYVDVSTDGGGTWTPIQGNITTASNPNGLNRGHGFTGSSGGWIDAI
                     FPLTAYLGADIMVRVVYVTDGGVLGSIGVRIDNIFPVPQCGSTTIVANGVTGGEYDFL
                     PPSTGLWRFRVRAVDAENHASGWSNSRDRDVTTLTGSDGPRAYVTDLGANYPNPFNPS
                     TRIPFVVGGALDGGETRIVLAIYSVTGARVATLVRESRRPGTYVAYWNGVDDTGRPAA
                     SGVYFARLDVGDARVSRKLVLLK"
     gene            4191..5474
                     /locus_tag="VEC56_06015"
     CDS             4191..5474
                     /locus_tag="VEC56_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXV13743.1"
                     /translation="MENRLKKQLALIKSRAITIARRGASVSEAPAAAPASDSSGHGTA
                     GEKSPPNSDNPIIGDLIRAGAVTRASDLEPDRFDISRRLRRGPWWWRRRLGRPQADPI
                     ARAARASAFESEFQRLFAGLANGESLSIESAVPGEARALEGSSYYLVRSRGPEIDPIA
                     PAEAGRFATLGSWPETVSRSMVGRFIRKGYAAHGFEDPRPDFATTIPLFRRADPHSIL
                     FLDIETAGLSANTYVFLCGLMRFDPETGTFEVEQAFARDYAEEEGLLLHVRSALERFD
                     TVVTYNGASFDMPFLRTRMAVHRIPDIVPMGSVDLLHATRRVFRPALPNVRLVTVEEH
                     LRGTARVDDIPSRFIPRAYHEYVRTQDARIMRNVVYHNRMDLFTMAVILNRLAAEGAP
                     RAGDPASPPAGAEKPFASASAGDTIASPNPPHVPF"
BASE COUNT          935 a         1796 c         1887 g         1016 t
ORIGIN      
        1 gcgctcgcgg cgtggaccgt gatccagggc gtgacggtgg ccgtgcacgt ggcgtggctc
       61 gtcgtcgacc cgggttatca gccggggctg gcgacgacgc cattgttcgc ggtcaccatc
      121 gcggcggcga tcgcggcgct caagcgcagg cgggttgcgg acggaaacag cgggaattag
      181 aacatcgcgg cggtcaaacc cggcgaggtg gcgcgcgcgg gggcttgcac caccgtcgtg
      241 cgatccaggc cttcgttgag cgcttcgtac atgcgggaga gatacgcgtc gcggatcgca
      301 ttgaaggcga cgatctcgct cggcgagacg ggctccttgg ccggcaagtc gagcatgcga
      361 gggttgaccc agcgcccgcc cttcttcatg cgatagcaca ggtgcggccc cgtcgcgagg
      421 ccggtcgagc ccacgtagcc gatcacttgc ccctgggtca cgcgcatgcc gggacgaacc
      481 cccttggcga agccggacag gtgcaggtag tacgtctcgt aggtcttgtt gtggtggatg
      541 cggacgtagt tgccgttgcc gctctggtag ttggcggcca gcacgagccc gtcgccggtg
      601 gcgtacacgg gcgtgccgcg cgcggcggcg tagtcgacgc cgtagtgcgg cttgtagacg
      661 cgctgcaccg ggtgcagacg gcggctcgaa tagttcgacg agatgcgcga gaatttcatg
      721 ggcgcgcggc gcatcgactt ttgcatcgat ttgccgtcga gcgcgtagta gcccaccgtc
      781 cctgattccg gcgtgtaccg aacggcgttg tactccttgc cgaggttgac gatgcgcgcc
      841 gccagcaccg ttccgagcgc ggtgtagccg gtctcgtacg tgcggcgctc gtagagcagc
      901 gcgtactggt cgccctgccg cagatccttc gccaagtcga gggtccagcc gaagatttcg
      961 tcgatcgatc ccgcgaggtc ggtctccacg ccctgcgact gcaaggaagc gaagatcgag
     1021 tcgtagatcg tgccgtgcgt gacgtgatag ctcacttcga agggcacttg ctcgacggcc
     1081 acggtgaagt actcgattcc gcggcgcacg cacacgaact cggccgggct ggtatataga
     1141 atgaggctgt cgatctcgcc ggcttcgttg gtatagaggt cgaaggtctg ccccttgcgg
     1201 acccggcgcc agtcgaagac cttcttggtc tcgcgcgtca tggtgtcgat ctcgaaggcg
     1261 gacaccccga gcgcgcgcag ctcgtcgtac atcgtactgt tcttgccgat ctcgccgcgc
     1321 accggctcgg gcttgacggg ggcggcgacg aaggccggac cgtctccatc ataggagtcg
     1381 gtcatgatca tcttcccgtc ctcggtatag aggcagccca ccgtctcggg cgcggggacc
     1441 ggaattcggt cccaaacggg ccgaaccacg ggctcctggc cggtgaaagc catgaccgcg
     1501 atgattccgg caacgagggg tgtggtgcgt ttctgcatgt aatctaactc acacttttta
     1561 agacggttag cgcccaaccg ggcggggcga atcgacccat catcggccgt ccgcaccacc
     1621 gggagcaagc cctttttggg actcccagta ctctggctgc aacttctatt ccgcgatggg
     1681 ggatttcgta tcgtgcccga tgcgggagag tagggtgcgg ccctcgggcc gcggtcggta
     1741 agaaaaccga atccgccggt cgggaacgca gaccggcgga ctcgtcaggg gcggcgaaga
     1801 cggactcacc gccaagagga agtgtgcacc tcggagcgct atttcagcaa gaccaacttg
     1861 cgcgagacgc gcgcgtcgcc gacgtcgagg cgcgcgaagt agacgccgga cgcggccggg
     1921 cgaccggtat cgtcgacgcc gttccaatac gcaacatatg ttcccggtcg gcgcgactcg
     1981 cgaaccagtg tggccacgcg cgcgcccgta accgaataga tcgccagcac gatgcgcgtt
     2041 tcgccgccgt cgagcgcccc ccccacgacg aacgggatgc gggtcgacgg gttgaacgga
     2101 ttgggatagt tcgcgccgag atcggtgacg tacgcgcgcg gtccatcgct cccggtcagc
     2161 gtcgtgacgt cgcgatcgcg gctgttgctc cacccactcg cgtgattctc ggcgtcgacc
     2221 gcgcgcacgc ggaagcgcca caaaccggtc gacggtggga ggaagtcgta ctcgccccca
     2281 gtgacgccgt tcgcgacgat ggtggtgctg ccgcactgag gcaccgggaa gatattgtcg
     2341 atgcgaacgc cgatggagcc gagcacgccg ccgtcggtca cgtacacgac gcgcaccatg
     2401 atgtccgccc cgagatacgc cgtgagcggg aagatcgcgt cgatccaccc gccgctcgat
     2461 cccgtgaacc cgtggccgcg attcaagccg ttgggattgc tcgcggtcgt gatgttgccc
     2521 tgaatcggcg tccacgtgcc gccgccgtcg gtgctcacgt cgacgtatcc gtaatcgtag
     2581 tcgacctcca cgttgtaatt gaccttgaaa cggagcgtat cggccgccgc gtcgaccacg
     2641 agcggctgcg ccatagtgat cgtgctgaac agattgttca gattgcccga atagtagccg
     2701 ccgccggaaa acccggtcag gctgtacgtg aagccgccca gggtccaccc ggtcgcggcc
     2761 ggcgtggcgg tgtcggtgaa ggtcgacggt tcgtggcagc cttcaatggt atacgacgcc
     2821 gcggggtttg ggtcctgcgg gtcctgcggc tcccacgaga tgcgcgtcac ggcgttgcca
     2881 aaaggcgcct gcacgacatt ctgctcgggt gcccacggtc ccaccacgcg gtaggggttg
     2941 tccgcgaacc ggagcagctt catgttcatc ggcaagagca ggttgaaggt cggcaggatg
     3001 tacgtttcat cgggaccgaa gccgccctgc gcgccgctgt tgatctcggg cgtgtacgca
     3061 aagatcttcg gcttcgacgt ttgctcgccg tagccccagt cgtcggaggc gccgttggtg
     3121 atgtagatcg cgccgctcgc ggggttgccg gcgaggtagc cattctccac cacgagcgtg
     3181 tcggccaagg ccgcgatcgc ctcgtggtcc ggcgtgttgg cggggatgta tccccagccg
     3241 tagaggagca gttcgccgta gctgtggtac gaaagccagg tcgtgaactc gcgttggttg
     3301 acgaagtttc gaatcgcctg cgtctccggc tccgagaact cctcggtgcc gcggtagacg
     3361 tcgctgctcg tgaccggcga cgacccgatg ttgtcgaagc cccactggta gccgtagttg
     3421 cgattgagat cgacaccgaa gaaaccgtcg aggttgtcac ggcggttctt tcgccaccag
     3481 gtgctccagt ggcccgcgtg gttcagttgc acgtagtagt gcccatcggg attcaccatc
     3541 gggacgacgt agatctcgcg cgaatcgacg tactcggtca cggtggcgtt gctgccgtag
     3601 ttctggagca ggtattgcgc gaacaagagc gggatctcga cgctcatcag ctcgcgagcg
     3661 tgatggttac ccatgtagag cacttcgggc tcgccctcgt cgagggcgac gttgtcggag
     3721 atcttgagcg cggtgatatt gcgaccctgg aggctcaagc cgatgaccga gcgttgcgcg
     3781 atggccggat acgtggacgc gagcgaggtc aacagcgcct cggtctcggc gtaggtgtgg
     3841 tactggccga gattcgcgtc cagcacggcg tggggctgcc ccggcttcac gacatggata
     3901 gagattgggt agggccggga tcgtaagtag tcgagtgccg cgccttcggc gagaacgtca
     3961 acgccgtact tggtataggc gaggatctcg atcccgcgcg cctcgaactc ggcgatttgc
     4021 gcggagccct tgaacggcac ggtaacgatt tcggagcgca cgagggacgg gacgagagcc
     4081 agcgcggccg taagcaagac cgccaggggg gaccggtgtc tcggagtcat gcaagattcc
     4141 ctttctctgg agggtggact tcgctcatta taatgcaaga cgggccgcgg atggagaacc
     4201 gactgaaaaa gcagctggcg ctgatcaagt cgcgggcgat caccatcgcc cggcgcggcg
     4261 cgagcgtttc cgaagcaccc gccgcggcac ccgcgagcga ttccagcggc cacgggaccg
     4321 cgggggagaa gtcccccccc aattccgaca acccgatcat cggcgatttg attcgcgcgg
     4381 gcgcggtgac gcgcgcctcc gatctggagc ccgaccgctt cgatatttcg cgccgccttc
     4441 gacgcgggcc gtggtggtgg cgtcgacgtc tcggccgccc gcaggccgac ccgatcgctc
     4501 gggccgcgcg cgcgtcggcg ttcgaatcgg aattccagcg cctgttcgcg ggcctcgcga
     4561 acggagaatc actctcgatc gagagcgcgg ttccgggtga agcccgcgcg ctcgagggat
     4621 cgtcgtatta tctcgtccgt tcgcgcgggc ccgagatcga ccccatcgct cccgcggaag
     4681 cgggtcgctt cgcgaccctg gggtcgtggc cggagaccgt ttcgcgatcg atggtggggc
     4741 ggttcattcg caagggctac gccgcgcacg gattcgagga cccacgaccg gatttcgcga
     4801 cgacgattcc gctgttccgc cgcgccgatc cgcattcgat cctctttctc gacatcgaaa
     4861 cggccggtct gtccgccaac acctacgtgt tcctgtgcgg gttgatgcgc ttcgacccgg
     4921 agacgggcac attcgaagtc gagcaggcct tcgcgcgcga ctacgccgaa gaggagggcc
     4981 tgctcctgca cgtgcgatct gcactcgagc gcttcgacac ggtggtcacc tataatggag
     5041 cgagcttcga catgccgttc ttgcgcacac gcatggccgt gcaccggatc cccgacatcg
     5101 tccccatggg ctcggtcgac ctgctgcacg ccacccgccg cgtgttccgg cccgcgctgc
     5161 ccaacgtccg cctggtgacg gtcgaggagc acttgcgcgg gacggcgcgt gtcgacgaca
     5221 tccccagccg gttcattccc cgcgcttacc atgagtacgt gcgcacccag gacgcgcgca
     5281 tcatgcgcaa cgtcgtctac cacaatcgca tggacctgtt cacgatggcc gtgatcctca
     5341 atcgcctcgc ggcggaggga gcgccgcgcg cgggcgaccc cgcttctccg cctgccggcg
     5401 ccgaaaagcc gtttgcatcc gcctccgccg gtgataccat cgcctctccg aaccccccgc
     5461 acgtgccttt ctagccgcgt gacgcccgcg tttctccgcg cgcgcgacgc gcaccacgag
     5521 gaatcgcgag catgaacgtc gacaagttta tccgccatat tcaatccgcc gagtggtacg
     5581 acggccaggt cgttcgcgtg gaatcgttgc cggagcgcgc cgcgcgcttc gcaa
//
LOCUS       DASPGK010000219        10576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_876408, whole genome shotgun sequence.
ACCESSION   DASPGK010000219 DASPGK010000000
VERSION     DASPGK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876408"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VEC56_06020"
     CDS             <1..751
                     /locus_tag="VEC56_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV13744.1"
                     /translation="FPLVFVRRDTLWKGTRPLRWVILAGACFAADLYFWHRSILYVGP
                     GLATLLGNFQVFFVAAAGLLVFHERATPRFLVSVPLALLGLFLLVGVRWGTFDADYRR
                     GVLFGILTALSYGAYLLCLRAARRERNRLSAVANLALSTVFTAAILGAVAGVRGESLH
                     IPSVRTASILVAYGVLCQALGWIIISRTLHVVDASRAALILMLQPALTYLWDVLFFRR
                     PMTAVEAGGAALAIAAIYLGNSRPAPAPPAT"
     gene            748..1164
                     /locus_tag="VEC56_06025"
     CDS             748..1164
                     /locus_tag="VEC56_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13745.1"
                     /translation="MNWTLILLLSLFGPVVGAFTVFGVFPRGVDRFVWMVVVGVCAWT
                     VAKREPARALPTGAVVGFITGASSTLVQALWVDTLAANNPWMVEALAGQPAGFDFEFF
                     VFMLVPFIGVAGGAMTGLLAMLAARALAARDAARKP"
     gene            1216..2700
                     /gene="pyk"
                     /locus_tag="VEC56_06030"
     CDS             1216..2700
                     /gene="pyk"
                     /locus_tag="VEC56_06030"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXV13746.1"
                     /translation="MTDIRVLRSPKSNVNATPPTLAGRERRTKIVCTLGPASESPSMI
                     ESLVHAGLNVARINASHGTPESHARMIANVRAVAKKLDAYVPVMLDLTGPKMRIGAIA
                     AGSATLRAGDSFTITSRTVPGDWREVSINYTPLVTDVNLGDRILMGDGEIELRVRTKT
                     ATDVECDVVVGGELKPNKGVNVPGIKLRETVPTEKDLLNVDFGIAHGVDYFALSFVRT
                     VEEVRRLRWVLREKGSDIPIIAKIEKREALDNLGPILDASDAVMIARGDLGLEMPIQQ
                     VPLIQKEVIRKALEASKPVITATQMLESMIQNPRPTRAEAADIANAVFDGTDAVMLSG
                     ETASGKYPIEAVRTMAEVAEASEARIEYAARFAQVAVDVTKSIPEAVAHAACFTALEI
                     GARVILCCTRSGQTALYVSNYRAPTKIAVISPHESTLKRTMLFWNTTSVKIEATRDTD
                     TMLQAAKDAVVAAGVAQRGDRVVVVAGVPVDVPGTTNMIKADVL"
     gene            2703..4151
                     /locus_tag="VEC56_06035"
     CDS             2703..4151
                     /locus_tag="VEC56_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV13747.1"
                     /translation="MLKDRYPYYVANAPVYANADLPVTDKFTGRTATRVALADDAAVD
                     TAIARALDAVEPMRALAAYERQAALAHCVRRFGERSEELAHALCVEAGKPIRDARGEV
                     ARLIDTFRVAAEESVRMVGETLPLDISARARDYRGMWKRVPLGVCSFITPFNFPLNLV
                     AHKVAPALAVGCPFVLKPASATPVGALIVGEVLAETKLPRGAFSILPVRGEKAARFAE
                     DARIRLLSFTGSPAVGWDLKARAGKKKVVLELGGNAAVIVDEDADLGDAVERIVFGAF
                     YQSGQSCIAVQRVLVHERVYDEVKRRLVEATSALRAGDPKQEDTFIGPMISEAEAQRL
                     SGWIEQAVERGARVLCGGTRKGAMLAATLLEGVPKDADACAKEAFGPVAVLSSFRDFD
                     AALDEVNDSVYGLQAGVFTRDVYKAHRAWDRLEVGAVVVGDVPSWRVDHMPYGGVKES
                     GQGREGVRFAMEEMTEIRLFVLRTPPMRSDET"
     gene            4183..5922
                     /locus_tag="VEC56_06040"
     CDS             4183..5922
                     /locus_tag="VEC56_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXV13748.1"
                     /translation="MKKFLLVLSMAMVVAVGFGCSKKGGAKKADAAFETLANAYIETL
                     LQMNPESATLLGDHRFDDRLNDRTIAGVTAQASANEEYLAKLQEIDRAQLSDANAVDY
                     DIMKLNLERGIFEALELREHEWNPLYYNMGGAVYSLVAREFAPIEQRLASVEARVRAI
                     PTALAAAKAQLQNPPKVFTETAILQNQGNISLLKEELAPFIQQAPAMEAQLKPAIDAS
                     VAALEEYGTWLEKDLLPRSNGEFRLGDEKWRRKLRFSLDSDLSKEDILARATVDLQAT
                     QQAMYATALPLYKKFFPEVTDESKLGDVKHVNKAVLDKLAEDRPDSDRLIHDAQVTLN
                     AAIAFTREKKLVTVPSEPVKIIVMPEFQRGVAVAYCETPGPLEPEGATLYAISPPPAT
                     WTPERTQSFFKEYNDYMLLNLTVHEAVPGHYLQGTHSNKFKAPTLVRAIFYSGSFAEG
                     WAVYTEKVMADAGFGGDEVRMQQLKMRLRVIINAIIDQKIHTEGMTEEEAMALMMNEG
                     FQEEGEAAGKWRRACLTSTQLSTYFVGSAEVEDIRKAYEAKHPNPDLLALHDQMLSYG
                     TPAPKYVKRMMGL"
     gene            6007..7194
                     /locus_tag="VEC56_06045"
     CDS             6007..7194
                     /locus_tag="VEC56_06045"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HXV13749.1"
                     /translation="MSGNATIVVGVPKETFPGETRVAMVPAVAAVLAKSGVQTVVERG
                     AGQASGYTDASYEAKGASLASRDEVFARAHVVVHVRVAGANAAGTRDDARRYRRGQIV
                     IGMADPLGEPEAVREFASTGASLMALELVPRITRAQSMDVLSSMATVAGYKAVLMAAN
                     ALPRMFPMMMTAAGTITPARVFVVGAGVAGLQAIASSKRLGAVVHAYDVRPAVKEQIQ
                     SLGAKFVELPVETANAEDKGGYARELGEEFYRKQRELMARVVAESDVVITTAMIPGKK
                     APVLITGEMLEKMAPGSVVVDLAAERGGNVDVTRAGETVQVGGVRVIGAVNLPASMPY
                     HASQMYARNVASFLQNMIKDNAIALSTDDEVVRDTLVTRDGDVASATLRERLGLATAQ
                     AAR"
     gene            7255..8328
                     /locus_tag="VEC56_06050"
     CDS             7255..8328
                     /locus_tag="VEC56_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HXV13750.1"
                     /translation="MRIGIIGLPQTGKKTLFSLLVGPAALSGHTDARAAARGMADVRD
                     PRFDRLVELYRPQRQVRARLEVVLCPSIEEKSLSEGEALKEISDVDALCHVVRVFEDD
                     AVYHIWGEPDPKREVEFLHGEMVLHDLVFIEKRLERIDKNLKKGKDDRAEKEKALLGR
                     FREALEKERPLRTVAVSPEDTGLIASYPFLTLRAIIVALNVADEAVADTRLRDALAAA
                     YEPTGASVIQIAVRAESEIAALEDPAERAAFMQDLGIETPALHLLTSKCIEALGLMSF
                     FTVGEDEVRQWFVRRGASAPEAAGAIHSDLQRGFIRAEVMKYADLITAGSEEKLKATG
                     KYYLKGKEYVVEDGDILTIRFNV"
     gene            8328..9509
                     /locus_tag="VEC56_06055"
     CDS             8328..9509
                     /locus_tag="VEC56_06055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXV13751.1"
                     /translation="MATVTLDSKRDRATRHRHPWIFAASIASVTGNPAPGDVVDVVDA
                     RGVLLGRGYHNEHSRIRVRVLTWDDRPVDDAFWRARVEAAIRRRGSLALGGDTNAYRL
                     IHAEADLLPGLIADRYGDVVVVQVLTAGIERARDVVVDALASLTEARCLYERSDTASR
                     TREGLPAVTGAIRGEAPRNIEVLENGHTFLVNIESGQKTGFYLDQRDNRAWVAAHAQG
                     CDVLDAFCHTGAFAVYCARAGAKSLSLIDSSAPSLGAARWNLERNGTGECAVAIQQAD
                     VFEELRAMRDAGRRFDLVVLDPPKFASNKHQVEKALRAYKDVNLLAMQLLVPDGLLAT
                     FSCSQAVDADSFTMAVSWAGVDAARDVQIIRRMGQGIDHPILATFPESEYLKGLLCRV
                     A"
     gene            complement(9517..10122)
                     /locus_tag="VEC56_06060"
     CDS             complement(9517..10122)
                     /locus_tag="VEC56_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXV13752.1"
                     /translation="MLRRSAEFADEVTARRSVRHFSSDPVPRGVILDCIRAAGQAPSG
                     ANKQPWTFVLVTNPEVKRRIREGAEAEEREHYGGRASERWLADLEPLGTDEHKPFLEV
                     APALIVVFAQRHGPRPDDQHYYVSESVGIAVGFLLAALHHAGLATLTHTPSPMKFLNQ
                     ILDRPGNERAYLLIPVGYPSTDCRVPDIARKPLSSYLVEVD"
     gene            10301..>10576
                     /locus_tag="VEC56_06065"
     CDS             10301..>10576
                     /locus_tag="VEC56_06065"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13753.1"
                     /translation="MGSQAFAIAAMFWIKHATESATLMGVVMMLGTLPFVLLGAIGGV
                     VADRESRKRILIACDLVAGAAVLLLSFAMTAGPHHTPLLIGCVCAVSV"
BASE COUNT         1772 a         3449 c         3636 g         1719 t
ORIGIN      
        1 gttcccgctc gtgttcgttc gccgcgacac gctgtggaag gggacgcggc cgctgcgctg
       61 ggtgatcctg gcgggcgcgt gcttcgcggc cgacctctat ttttggcacc ggtcgatcct
      121 gtacgtgggt cccggcctcg ccacactgct cggcaacttc caggtcttct tcgtggccgc
      181 ggccgggctg ctcgtgttcc atgagcgcgc gacaccccgc ttcctggtgt cggtgccgct
      241 cgccctcctg gggttgtttc tcttggtcgg tgtgcggtgg ggcaccttcg acgcggacta
      301 tcggcgcggc gtgctcttcg ggattctcac cgcgttgagt tacggcgcct acctgctgtg
      361 cttgcgcgcc gcgcgccgcg agcgcaatcg actgtccgcg gtcgccaacc tggccctgtc
      421 gaccgtgttc accgccgcga tcttgggtgc ggtcgccggc gtgcgcggcg agtcgctcca
      481 catcccgagc gtgcgcacgg catcgattct ggttgcatac ggggtgctgt gccaggccct
      541 ggggtggatc atcatctcgc gcacgctgca cgtggtcgac gcatcgcgcg ccgcgctcat
      601 tctcatgctg cagcccgcgc tgacgtacct ctgggacgtg ctcttcttcc ggcgcccgat
      661 gaccgcggtc gaagcggggg gcgcggcgct ggccatcgcc gcgatctact tggggaactc
      721 gcgaccggcc cccgccccgc cggcgacatg aactggacgc tcattcttct tctctcgctg
      781 ttcggacccg tggtgggggc gttcaccgtc ttcggggtgt tcccgcgcgg ggtggaccgc
      841 ttcgtgtgga tggtcgtggt cggcgtgtgc gcgtggacgg tggcgaagag ggaacccgcg
      901 cgcgctctcc ccaccggcgc cgtggtcggt ttcatcaccg gggcgtcgtc cacactggtc
      961 caggcgctgt gggtcgacac gcttgcggcc aacaacccgt ggatggtcga agccctggcc
     1021 gggcagccgg ccggcttcga cttcgagttc ttcgtcttca tgctggttcc cttcatcggt
     1081 gtggcgggcg gcgcgatgac ggggctcctg gccatgctcg ccgcgcgcgc gctggcggcc
     1141 cgcgacgccg cgcgcaagcc gtaggccggg ttgtcccttg acaacgcacg ccgcatgccc
     1201 aacgctaggc ctgcaatgac cgacatccgc gttctccgat cccccaagtc aaatgtcaac
     1261 gcgacgccgc ccaccctggc cgggcgcgag cgccgcacca agatcgtgtg cacgctcggc
     1321 cccgcgagcg aatcgccctc catgatcgag tcgctggtcc acgccggctt gaatgtggcg
     1381 cgcatcaacg cgtcccacgg cacgcccgag agccacgcgc gcatgatcgc caacgtgcgc
     1441 gcggtggcga agaagctcga cgcgtacgtt cccgtgatgc tcgatctgac cggccccaag
     1501 atgcgcatcg gcgccatcgc ggccgggtcg gccacgctgc gcgcgggcga ctccttcacc
     1561 attaccagcc ggacggtgcc cggcgactgg cgcgaagtat cgatcaacta cacgccgctc
     1621 gtcaccgacg tcaacctggg cgatcgcatt ctcatgggcg acggcgagat cgagttgcgg
     1681 gtgcgtacga agaccgcaac cgacgtcgaa tgcgacgtcg tggtcggcgg cgaactcaag
     1741 cccaacaagg gagtcaacgt cccggggatc aagttgcgcg agacggtgcc cacggagaag
     1801 gaccttctca atgtggactt cgggatcgcg cacggcgtcg attactttgc gctctcgttc
     1861 gtacgaacgg tggaagaagt gcggcgcctg cgctgggtgt tgcgcgaaaa gggatccgac
     1921 atccccatca tcgccaagat cgagaagcgc gaggcactcg acaacctggg gccgatcctc
     1981 gacgcctccg acgcagtcat gatcgcgcgc ggtgatttgg ggctggagat gcccatccag
     2041 caggtgccgc tgatccagaa ggaagtgatt cggaaggcgc tggaagcgag caaacccgtc
     2101 atcacagcca cccagatgct ggagtcgatg atccagaacc cgcgcccgac gcgcgcggag
     2161 gcggccgaca tcgccaacgc ggtcttcgac gggaccgacg cggtgatgct ctcgggcgag
     2221 accgcgtcgg gcaagtaccc catcgaagcg gtgcgtacca tggcggaagt cgcagaggcg
     2281 agtgaggcgc gcatcgagta cgccgcgcgg ttcgcgcagg tcgcggtcga cgtgaccaag
     2341 agtatccccg aagcggtcgc gcacgcggcc tgcttcaccg cgctcgagat cggcgcgcgc
     2401 gtcattctgt gctgtacgcg ctccggacaa accgcgctgt acgtgtccaa ctaccgcgcg
     2461 cccaccaaga ttgccgtcat cagcccgcac gagtccacgc tcaagcgcac catgctgttc
     2521 tggaacacga cgtcggtgaa gatcgaagcc acgcgcgata ccgacaccat gctccaggcc
     2581 gccaaagacg cggtggtggc cgcgggtgtg gcgcagcgcg gcgaccgcgt ggtggtcgtc
     2641 gcgggcgtgc ccgtcgacgt tcccggcacc accaacatga tcaaagccga cgtgctgtga
     2701 acgtgctcaa ggatcgctac ccctactacg tcgccaacgc tcccgtttac gcgaacgccg
     2761 acctgccggt gaccgacaag ttcacgggcc gcaccgcgac gcgggtggca ttggcggacg
     2821 acgccgccgt cgacaccgcg atcgcgcgcg ccctggacgc ggtggagccc atgcgcgccc
     2881 tcgcggccta cgagcgccag gccgcgctgg cacactgcgt gcgccgcttc ggcgagcgct
     2941 cggaggagct ggcgcacgca ctgtgcgtcg aggccggcaa gcccatccgc gacgcgcgcg
     3001 gtgaggtcgc gcgcttgatc gacacctttc gcgtggcggc ggaagaatcc gtgcgcatgg
     3061 tgggcgagac gctcccgctc gacatctccg cgcgcgcacg ggactaccgc ggcatgtgga
     3121 agcgcgtgcc gctgggtgtg tgctcgttca taacgccgtt caactttccg ctcaacctgg
     3181 tcgcgcacaa ggtggccccc gcactcgccg tcgggtgtcc cttcgtgctc aagcccgcga
     3241 gtgcgacccc ggtgggcgcg ctcatcgtcg gcgaggtgct ggccgagacc aagctgcccc
     3301 gcggcgcgtt ctcgatcctg cccgtgcgcg gggagaaggc ggcgcgcttc gcggaagacg
     3361 cgcgcattcg tctgttgagt ttcaccggat cgccggcggt cggttgggac ttgaaagcgc
     3421 gcgccggaaa gaagaaagtg gtgctcgagc tgggcggcaa cgcggccgtg atcgtcgacg
     3481 aggacgcgga tcttggcgac gcggtcgagc ggatcgtgtt cggcgccttc tatcaatccg
     3541 ggcagagctg catcgcggtc cagcgcgtgc tcgtgcacga gcgcgtctac gacgaggtca
     3601 agcgccgcct ggtcgaagcc acgtccgcgc tgcgcgcggg tgatcccaag caagaggaca
     3661 ccttcatcgg gcccatgatc tcggaggcgg aggcacagcg gctctccggg tggatcgagc
     3721 aggcggtgga gcgcggcgcg cgcgtgctct gcggcggaac gcgcaaaggt gctatgctgg
     3781 cggccacgct gctcgagggc gtccccaaag acgccgacgc gtgcgccaaa gaagcctttg
     3841 gtccggtagc cgtgctgtcg tcgtttcgcg atttcgacgc cgcgctcgac gaagtcaacg
     3901 acagcgtgta cggtctccag gcgggtgtgt ttacccgcga cgtgtacaag gcgcaccgcg
     3961 cgtgggaccg attggaagtg ggtgcggtgg tggtggggga cgtgccgtcg tggcgcgtcg
     4021 accacatgcc gtacggcggc gtgaaggaaa gcgggcaggg acgcgagggg gtgcggttcg
     4081 cgatggagga aatgacggag atccgtcttt tcgtcctacg cacgccgccg atgcggtccg
     4141 acgagacgta gcccgcggga cttgaccaag gagaggcgac gaatgaagaa gttcctgctc
     4201 gtgctaagca tggccatggt ggtcgcggtc ggcttcggtt gctccaagaa gggcggagcc
     4261 aagaaggccg acgcggcgtt cgagacgctc gcgaacgcct atatcgagac cttgctgcag
     4321 atgaaccccg agtcggccac cttgctgggc gaccaccgct tcgacgatcg cctcaacgat
     4381 cgtacgatcg cgggtgtcac cgcgcaggcc agcgccaacg aggagtatct cgcgaagctc
     4441 caggagatcg accgcgccca attgagcgac gccaacgcgg tcgactacga catcatgaag
     4501 ctcaacctcg agcgcggcat cttcgaggcg ctggagttgc gcgagcacga atggaatccg
     4561 ctctattaca acatgggcgg tgcggtgtac tcgttggtgg cgcgtgaatt cgcgcccatc
     4621 gagcagcggc tcgcgtcggt cgaggcgcgc gtcagggcta tcccgaccgc cctggccgcg
     4681 gccaaggccc agctccagaa cccgcccaag gtgttcacgg agaccgccat cctgcagaac
     4741 caaggcaata tcagcttgct caaggaagag ctcgcgccct tcatccagca ggcgcccgcg
     4801 atggaggccc agctcaagcc cgcgatcgac gcctcggttg ccgccctgga agaatacggc
     4861 acctggctcg agaaggatct cctcccgcgg tcgaacgggg aattccgact gggcgacgag
     4921 aagtggcgca ggaagctgcg tttctcgctg gactcggatc tttcgaagga agacatcctc
     4981 gcgcgcgcaa ccgtcgactt gcaggcgacc cagcaggcga tgtacgcgac cgcgctcccg
     5041 ctctacaaga agttcttccc cgaggtcacc gacgaatcca agctcggcga cgtgaagcac
     5101 gtcaacaagg cggtgctcga caagctggcc gaggatcgtc ccgacagcga ccgcctcatt
     5161 cacgatgctc aagtgaccct caacgcggcc atcgcgttca cgcgcgagaa aaaactcgtc
     5221 acggtaccga gcgaaccggt aaagatcatc gtgatgccgg agttccaacg cggcgttgcg
     5281 gtcgcgtact gcgagacacc cggaccgctg gagcccgaag gcgccacgtt gtacgctatc
     5341 tcgcctccac ccgcgacctg gacgccggag cgcacgcagt cgttcttcaa agagtacaac
     5401 gactacatgc ttctcaacct caccgtgcac gaagccgtgc ccgggcatta tcttcagggc
     5461 acgcactcga acaagttcaa ggcgcccacg ctggtgcgcg cgattttcta cagcggctcg
     5521 tttgctgaag ggtgggccgt gtacacggag aaggtcatgg ccgacgccgg cttcggcggc
     5581 gacgaggtgc gcatgcagca gctcaagatg cgcctgcgcg tcatcatcaa cgccatcatc
     5641 gaccagaaga tccacaccga gggcatgacc gaagaagagg cgatggccct catgatgaac
     5701 gagggcttcc aggaagaggg cgaagccgcc ggcaagtggc gccgcgcgtg cctcacctcg
     5761 acccagttgt cgacctactt cgtcgggagc gcggaggtcg aggacatccg caaggcgtac
     5821 gaagcgaagc acccgaaccc ggacctcttg gcgctccacg accagatgct gtcctacggg
     5881 acgccggcgc ccaagtacgt caagcgcatg atggggctgt agacgccgcg acggcggcgt
     5941 ggtaagttcg cgagggaagc gctcggtgct cgcccttcat ttggtgccgc aagagatgag
     6001 gatactatgt caggtaacgc tacgattgta gtcggcgtcc cgaaagagac gttcccgggc
     6061 gagacgcgcg tggccatggt gcccgccgtc gccgccgtgt tggccaagtc cggcgtgcaa
     6121 accgtcgtcg agcgcggcgc aggccaggcg tcggggtaca ccgacgcctc ttacgaggcc
     6181 aagggcgcgt cgctcgcttc gcgcgacgag gtattcgcgc gcgcccacgt cgtcgtccac
     6241 gtgagagtgg ccggcgccaa cgcggcgggc acgcgcgacg acgcgcgtcg ctaccgtcgc
     6301 ggccagatcg tgatcggcat ggccgacccg ctcggcgagc ccgaagccgt gcgcgagttc
     6361 gcgtcgacgg gtgcgtcgct catggcgctc gagctcgtcc cgcgcatcac gcgcgcgcag
     6421 agcatggacg tgctctcgtc catggcgacg gtggccgggt acaaggccgt gttgatggcg
     6481 gccaacgcgc tgccgcgtat gttcccgatg atgatgacgg ccgccggcac catcaccccc
     6541 gcgcgcgtgt tcgtggtggg cgcgggcgtg gccggcctgc aggccatcgc gtcgtcgaag
     6601 cgcctgggcg cggtcgttca cgcctacgac gtccgcccgg cggtcaagga gcagatccag
     6661 agcctcggtg ccaagttcgt cgagctcccg gtcgagacgg ccaacgcgga agacaagggt
     6721 ggctacgcgc gcgagctggg cgaagagttc tatcgcaagc agcgcgaatt gatggcgcgc
     6781 gtggtggccg agagcgacgt cgtcatcacg accgcgatga ttccgggcaa gaaggcgccg
     6841 gtgttgatca cgggcgagat gctcgagaag atggcgcccg gctcggtggt ggtcgacctg
     6901 gccgcggagc gcggcggcaa cgtcgatgtg acgcgcgcgg gcgagaccgt gcaggtgggc
     6961 ggggtgcgcg tcatcggcgc cgtcaatttg cccgcctcga tgccgtacca cgcgagccag
     7021 atgtacgcgc gcaacgtcgc gtcgttcttg cagaacatga tcaaggacaa tgcgatcgcg
     7081 ctgtcgaccg acgacgaagt cgtgcgcgac acgctggtga cgcgcgacgg tgacgtcgcg
     7141 agcgcgaccc tccgcgaacg cctggggctg gcgacggcgc aagccgcccg gtagcctggc
     7201 gtcgcggatc gactccgccg gcaagaattt ctcctaaccg ttccacgaaa tcctatgagg
     7261 atcggcatca tcggtcttcc gcagaccggc aagaagacgt tgttctcgct gctggtggga
     7321 ccggccgcgc tgtcggggca caccgatgcg cgcgcggccg cgcgcgggat ggccgacgtc
     7381 cgcgacccgc gcttcgaccg gttggtcgag ttgtaccgac cccaacgcca ggtgcgcgcg
     7441 cgcctggaag tcgtgctgtg tcccagtatc gaagagaagt cgctttcgga gggcgaagca
     7501 ttgaaggaga tctccgacgt cgacgcgctg tgtcatgtgg tgcgtgtgtt cgaggacgac
     7561 gccgtgtacc acatctgggg ggagcccgac cccaagcgcg aagtcgagtt cctgcacggc
     7621 gagatggtgc tgcacgacct cgtgttcatc gagaaacgcc tggagcgcat cgacaagaat
     7681 ctgaagaagg gcaaagacga ccgcgccgag aaggagaagg cgctgctcgg gcgcttccgc
     7741 gaagcgctcg aaaaagagcg accattgcga accgtcgccg tctcgcccga ggacacgggg
     7801 ctgatcgcga gctacccgtt tctcaccctg cgcgcgatca tcgtggcgct gaacgtcgcc
     7861 gacgaggcgg tggccgacac gcggctgcgc gatgcgctgg cggctgcgta cgagcccaca
     7921 ggtgccagcg tgatccagat cgcggtgcgc gccgagtcgg agatcgcggc tctcgaagac
     7981 cccgcggagc gagccgcgtt catgcaggac ctgggcatcg agacgcccgc gctgcacctg
     8041 ctcacctcga agtgcatcga agcgctgggg ctgatgtcgt tcttcacggt gggtgaggac
     8101 gaagtgcggc aatggttcgt gcgccggggt gcctccgcgc cggaggccgc gggcgccatc
     8161 cacagcgatc tgcagcgcgg cttcattcgc gccgaagtga tgaagtacgc cgacctcatc
     8221 acggccggga gtgaagagaa gctcaaagcc accgggaagt actacctcaa gggcaaagag
     8281 tacgtcgtcg aagacggcga catcctcacc atccgcttca acgtgtgatg gctaccgtca
     8341 cgctggattc caagcgcgac cgcgccacac gccaccgcca cccgtggatc ttcgccgcta
     8401 gcatcgcgtc ggtgacgggg aatcccgcgc ccggcgacgt cgtcgacgtg gtcgacgcgc
     8461 gcggcgtcct cctcggccgc ggataccaca acgagcactc gcgcattcgc gtgcgcgtgc
     8521 tgacgtggga cgaccggccc gtcgacgacg ccttctggcg cgcgcgcgtg gaagccgcga
     8581 tacggcggcg cggctcgctc gcgctcggcg gcgacaccaa cgcgtaccgg ttgattcacg
     8641 ccgaagccga ccttcttccg gggctcatcg ccgatcgcta cggagacgtg gtcgtcgtgc
     8701 aggtgctgac cgccgggatc gagcgggcgc gcgacgtcgt ggtcgacgcg ctcgcatcgc
     8761 tcacggaagc gcgctgtctc tacgagcgca gcgacaccgc gtcgcgcacg cgcgaagggt
     8821 tgcccgcggt gacgggcgcg atccggggcg aggcgcccag gaacatcgaa gtgctcgaga
     8881 acgggcatac cttcctggtc aacatcgaga gcggacagaa aaccgggttc tatcttgatc
     8941 agcgcgacaa tcgcgcttgg gtcgcggcgc acgcgcaagg ctgcgacgtc ctggacgcgt
     9001 tctgccatac cggcgcattc gccgtgtact gtgcgcgcgc gggcgcgaag tcgctttcgc
     9061 tgatcgattc gtcggcgccc tcactcggcg ccgcgcggtg gaacctggag aggaacggaa
     9121 ccggcgagtg tgccgtcgcg atccagcaag cggacgtgtt cgaggagctg cgcgcgatgc
     9181 gcgacgcagg ccgtcgcttc gacctagtgg tgctggaccc gcccaagttc gcgagcaaca
     9241 agcaccaggt cgagaaggcg ctacgagcct acaaggacgt caacctgctc gccatgcagc
     9301 tgctggtgcc ggacggcttg ctggcgacat tctcgtgctc gcaagcggtc gacgcggact
     9361 cgttcacgat ggcggtctcg tgggccggcg tggacgccgc gcgcgacgtg cagatcatcc
     9421 ggcgcatggg gcagggcatc gaccacccaa ttctcgccac ctttccggag tcggagtact
     9481 tgaaggggct gttgtgccgc gtcgcgtgaa gggcgcctag tcgacttcga ccagatacga
     9541 cgagagcggc ttgcgcgcga tatccgggac ccggcagtcg gtcgacggat acccgaccgg
     9601 gatcagcagg tacgcgcgct cgttgccggg ccggtccagg atctgattca ggaacttcat
     9661 cgggctcggc gtgtgcgtga gcgtcgcgag gccggcgtgg tggagagccg cgagcaggaa
     9721 gccgaccgcg attcctaccg actcgctgac gtagtagtgc tgatcgtccg ggcgcggtcc
     9781 gtgtcgctgc gcgaagacga cgatcaaagc gggtgcgact tccaggaacg gcttgtgctc
     9841 gtcggtaccg agcggctcga ggtccgcgag ccagcgctcg gaggcgcgcc cgccatagtg
     9901 ctcccgttcc tccgcttccg ccccctcgcg gattctccgc ttcacctcgg gattggtcac
     9961 caggacgaac gtccacggct gcttgttggc cccggacggc gcttgaccgg cggctcgaat
    10021 acagtcgagg atgacaccgc gcgggacggg gtcgctcgaa aagtggcgca cggagcgacg
    10081 ggcggtgact tcgtctgcaa actccgccga cctgcgcagc atctcttcgg gagagcgccg
    10141 ggtgaagggg aggcgaacgg agcggaaacg gggctcggag gcgtcgtggt tcatggcgcg
    10201 attgtaagga gaatgttcga tcgctacaag taacgggatt ccgcgccggc tctggaaccg
    10261 aaacttcgtc ctggtctggc agggccagat ggtgagccgg atggggtcgc aggccttcgc
    10321 catcgccgcg atgttctgga tcaagcacgc caccgaatcg gccaccttga tgggggtggt
    10381 catgatgctc ggcaccttgc cgttcgtgct gctgggagcc atcgggggcg tggtcgccga
    10441 ccgcgagtcg cgcaagcgca tcctgattgc gtgcgatctc gtcgcgggcg ccgccgtact
    10501 gctgctctcc ttcgccatga cggccgggcc gcaccacacc ccattgctga tcggttgcgt
    10561 gtgcgccgtg tcggtg
//
LOCUS       DASPGK010000220         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_993361, whole genome shotgun sequence.
ACCESSION   DASPGK010000220 DASPGK010000000
VERSION     DASPGK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993361"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..805
                     /locus_tag="VEC56_06070"
     CDS             332..805
                     /locus_tag="VEC56_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13754.1"
                     /translation="MSLAGVDAPRATRYVDLSIDQLTRNASVIFEGQVTGIRSDWNAD
                     RTRIYTTVTVRVDQYHKGNLGRGVVDVRFAGGSVGDIGMAVVGQPTFAPDERVFLFLR
                     PNFDVRDTPIVGDAAGKMRVATDASGADILLGPAATFEKDDVVREIRSVLRPIGQ"
     gene            818..3493
                     /locus_tag="VEC56_06075"
     CDS             818..3493
                     /locus_tag="VEC56_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13755.1"
                     /translation="MKKTITILLAIGCILVATTRVNAQAIPRVLAPDPAALLAELQLH
                     EWPPMMHWDLREFPGCDVPWAMNPNPVPDLDGNGADNTAADRAAARAAFAASFAAWEA
                     VAPSIIGFVESGTPALGGPGMDGYNTIGFGDGAMDDVQVVAPGNPVAAGGVVVGPGPN
                     GVLESQPGGDDIVAGTDIVDGGNLVAESFANTYGSVVGALAFTVLFFDMPTGRIIESD
                     ITFDPALAWVVNPATNACSPPAAVAAFNVAGTAVHEIGHFIGIAHPINPNPGAADAAD
                     GITPTMHPTLCPAFGGNNFDQTLENADRDPCNFLYTPDLGDAPDPWMGVFNQYPTLVH
                     LVGQGRVLNELQLDARAEGAEHIFGIKPVQGARNWTYEWLARFNGPGVTPECEANIVD
                     RDPLDDGVTWYPNPPIWGRLLTVTAWIKYASDAQGNAHNYPARGLFANAWIDLNQNCV
                     WEEWFMSVGQAPPPPLGPNTILTTTATASVFLPPFIDPTRPVWLRARVDYGEHVGIIN
                     NIDGTLNAERGAAQFGEVEDYPMHCRTKYEQQWTQNVLPYPTTGTSLVFVGAPDPAEQ
                     FYSAIVDDNDCPIETAPLPIVSYVPSLDETITEYPVPTTVPPGGRKHSGKCKPNNPPG
                     QPPLTLLRSHWVTDQVPAQTPAHLVPPELRIPSVNCEAFSALDNDTGAIDFVRWSVGA
                     VDRGSGGWISMIDEENHVWDDQLTVTVGYRLSPTLLPLASLSPCDPAYAALPLTMVGT
                     GTVTPEDALSDIIEEIPIPSYLILEVEVSWNTNGTLNRQIIEFPDPFGEPTPTDDSPL
                     PKSLVLESYPNPFNPSTTIRFALPKAETVSLAVYDVTGRLVRTLLDDARKPAGFFEAQ
                     WNGTDSNGRQVASGVYFFRLTTSTETLTRKAVLLK"
     gene            3695..4189
                     /locus_tag="VEC56_06080"
     CDS             3695..4189
                     /locus_tag="VEC56_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV13756.1"
                     /translation="MPQNPLSGSEICQRLDELAAGGAGFDSVLERAVDLVHRSNPRFH
                     WTGIYELFPDDVLRLGPFVGAPTDHVFISVGRGVCGSAVAEGRDKNVPDVSKEPNYLA
                     CSSATKSELVVLIRKGDTIFAQIDIDSHDIDAFDARTEREVRVVADWLAAKYEARRRA
                     NAGA"
     gene            4211..>5618
                     /locus_tag="VEC56_06085"
     CDS             4211..>5618
                     /locus_tag="VEC56_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV13757.1"
                     /translation="MPKKSSGVQLESVDALRRRVEELERWFQTHDRQVRFLERERQKL
                     SALVNHTDAGFVVFDPSLRVRWLNNICRAWFGGMQSAHAGRTASCNELLCGEHAICAA
                     CPTAQVLATGTVSHREVRLDVDGVTRHIYATAMPIKSHDNRVDEAILMLQDITDLEVL
                     RRSEEASMRAKEAAESASRAKSEFLANMSHEVRTPMTGVLGMTGLLLDTELTPEQRDY
                     VRIVQSSAEALLAIINDILDFSRIEAGRLTIDPIPFDLVAATDEVVALIAERAEEKGI
                     ELIVRYAPDVPRRVVGDAGRIRQVLMNLAGNALKFTHEGYVLISIEKLDGDDESARVR
                     VSIEDTGIGIEHDKLDAVFEKFTQADGSITRRYGGTGLGLAITKELIELMDGEITVTS
                     TPKSGSTFAFTLTFPLDREAPMEPPQPENLEGVRVLVLHRHEKVRGVLHEQVVSWKAR
                     VTAVATGAEAIAELEAAYR"
BASE COUNT          968 a         1933 c         1816 g          901 t
ORIGIN      
        1 cgaatggcgc cgcttccgcg ccgacgcccg cgagacgctg ggcgcccgct tccccgacgg
       61 agtcacatac acccggcggg tgttcattgc aaccggacgg ttgtcatcgc gataactcgg
      121 tggtatactc cccgacgatg acgaccacct gctagccgta ccgaaccgcg gcgctccctg
      181 ggtcgaagtt catcccgcct gccgcccgca ccggccgcca tccagttttc cgaagttcct
      241 cgtttgcccc caacgttcgc gtgaccgctc gcgtcgtgtt gcgcgatgga ggtgcttcat
      301 gcgcgcagtg tttcttgcca ccgtactcac ggtgtcgctc gccggcgtag atgcgccgcg
      361 ggccactcgc tacgtcgatc tatcgatcga ccagcttacc cgcaacgcgt cggtgatatt
      421 cgaaggtcaa gtcaccggaa ttcgcagcga ctggaacgcg gaccgcacgc ggatctacac
      481 caccgtcacg gtccgcgtcg atcagtacca taagggtaat ctcggccgcg gcgtcgtcga
      541 tgtgcgattc gccggcggct cggtcggaga catcgggatg gcggtcgtgg gtcagccgac
      601 gttcgcgccc gacgagcgcg tgttcctgtt cctgcgcccg aacttcgacg tgcgcgacac
      661 gcccattgtg ggcgacgcgg caggcaagat gcgcgtcgcg accgacgcct ccggcgccga
      721 catcctgctc ggccctgcgg cgacgttcga gaaggacgat gtggtgcgcg aaatccggtc
      781 ggtgctccgg ccgatcgggc agtaggaggg atcgacgatg aaaaagacga tcacgatcct
      841 gctggcaatc ggatgcattc tagtcgcaac cacgcgcgtg aacgcgcagg cgatcccgcg
      901 cgttctcgcg cccgatccgg ccgccctcct cgcggagctc cagctccacg agtggccgcc
      961 gatgatgcac tgggacctgc gcgaattccc gggctgcgac gtgccgtggg cgatgaatcc
     1021 caatcccgtc cccgacctgg acggcaacgg ggccgacaat accgccgcgg atcgcgcggc
     1081 tgctcgcgcc gcgttcgcgg cgtcgttcgc ggcgtgggaa gcggtcgcgc cgtccatcat
     1141 cggcttcgtc gaatcgggaa cgcccgcgct cggcggaccc ggaatggacg gttacaacac
     1201 catcggtttc ggcgacggtg ccatggacga cgtgcaggtg gttgctcccg gcaacccggt
     1261 ggccgcgggc ggtgtggtgg tggggccggg ccccaacggc gtattggagt cgcagccggg
     1321 cggcgacgac atcgtcgccg gcaccgacat cgtcgacggc ggcaacctcg tcgcggagtc
     1381 gtttgccaac acctatgggt cggtagtggg cgcgctcgcg ttcacggtgc tgttcttcga
     1441 catgccgacc ggccgcatca tcgagtccga catcacgttc gaccccgccc tggcgtgggt
     1501 cgtcaacccc gccaccaacg cctgctctcc cccggcggcc gtcgcggcct tcaacgtggc
     1561 cggtacggcg gtgcacgaga tcgggcattt catcggcatc gcgcacccga tcaatccgaa
     1621 ccccggggcg gccgatgcgg ccgacggaat cacgccgacc atgcacccca ccctgtgccc
     1681 cgcgttcggc ggcaacaact tcgatcagac cctggagaac gccgaccgcg acccgtgcaa
     1741 tttcctctac acgcccgatc tgggcgacgc tcccgacccg tggatgggcg tgttcaatca
     1801 gtatccgacg ctggtgcact tggtcgggca gggccgcgtg ctcaacgagc tccagctcga
     1861 cgcgcgcgcg gagggcgccg agcacatctt cggcatcaag ccggtgcagg gcgcccgcaa
     1921 ctggacatat gagtggctgg cgcgattcaa cggccccggc gtcacaccgg agtgcgaggc
     1981 gaacatcgtc gaccgcgacc cgctcgacga cggcgtcacg tggtacccga atccgcccat
     2041 ctgggggcgc ctgctcacgg tcacggcgtg gatcaagtac gcgagcgacg cgcaggggaa
     2101 cgcgcacaac taccccgcgc gcggtttgtt cgcgaacgcg tggatcgact tgaatcagaa
     2161 ttgtgtttgg gaagagtggt tcatgtcggt gggacaggcg ccgccgccgc cgctcgggcc
     2221 caacacgatc ctgaccacca cggccacggc ctccgtgttc ctgcccccgt tcatcgaccc
     2281 cacgcggccg gtgtggctgc gcgcgcgcgt cgactacgga gagcacgtcg ggatcatcaa
     2341 caacatcgac ggcacgctga acgcggagcg gggcgcggcg cagttcggcg aagtcgaaga
     2401 ctatccgatg cattgccgca ccaagtacga gcagcagtgg acgcaaaacg ttctgccgta
     2461 cccgactacc ggcacgagcc tggtgttcgt cggtgctccg gatccagccg aacagttcta
     2521 cagcgcgatc gtcgacgaca acgactgccc gatcgaaacg gcgcccctgc cgattgtgag
     2581 ctacgtgccg tcgctggatg agacgatcac cgagtatccg gtgccgacca cagtgccccc
     2641 gggaggtcgc aagcattcgg gcaagtgcaa gcccaacaac ccgccggggc agccgccgct
     2701 caccttgctg cgctctcact gggtgacgga ccaagtgccc gcgcagacgc cggcccacct
     2761 ggtgccgccg gagttgcgga ttccgagcgt gaattgcgaa gcgttctcgg ccttggacaa
     2821 cgacaccggc gccatcgact tcgtacgatg gtcggtcggc gccgtcgacc gcggaagcgg
     2881 cggatggatt tcgatgatcg acgaggagaa ccatgtttgg gacgaccaac tcacggttac
     2941 cgtcggatat cgactttcgc cgacgctgct cccgctcgcg agcttgagtc cttgcgatcc
     3001 ggcatacgcg gcactgcccc tcacgatggt tggaactggg accgtgaccc ccgaggacgc
     3061 cctttccgac atcatcgaag agattcccat tccctcctat ttgatcctcg aagtcgaggt
     3121 atcctggaac acgaacggaa ccctgaaccg tcagatcatc gagttcccgg acccgttcgg
     3181 cgaacccacg cccaccgacg actcgccgct gccgaagagc ctcgtgctcg agagctaccc
     3241 gaacccgttc aacccctcga ccacgattcg cttcgcgcta ccgaaggcgg agacggtgtc
     3301 gctcgcggtg tacgacgtga cggggcgctt ggtgcgcacg cttctcgacg acgcgaggaa
     3361 gcccgccggc ttcttcgaag cgcagtggaa cgggaccgac tcgaacggca ggcaagtcgc
     3421 gtcgggcgtg tatttcttcc gcctgacgac gagcacggaa accctcacgc gtaaggcggt
     3481 gctcctgaag tagcgccttc gcccccatcg tgaggacgcg acgtcgggcg cggtgacgcc
     3541 gggaggccgg tgctccgcgc ccgaagtcgt tgcggggctg ctgcatcccg gcatcatggc
     3601 atgggagttg cggtccggag catccatgcg tcgataggac tcggcccgca ctccgactag
     3661 tcccattatc gacccgcact tacggaggtt tcccgtgccc cagaacccgc tcagcggttc
     3721 ggaaatctgc cagcggcttg acgagcttgc ggccggtggc gccggattcg attcggtcct
     3781 cgagcgcgcc gtcgacctcg tccaccgatc gaacccgcgc ttccactgga ccggcatcta
     3841 cgagttgttc cccgacgacg tcctgcgcct gggcccgttc gttggcgcgc ccaccgacca
     3901 cgtcttcatc tcggtggggc ggggtgtgtg cgggagtgcg gtggccgaag ggcgcgacaa
     3961 gaacgtgccc gacgtgtcga aagagcccaa ttacctcgcc tgctcgagtg ccaccaagtc
     4021 ggaattggtc gtgctgatcc gcaagggcga caccatcttc gcccaaatcg acatcgacag
     4081 ccacgacatc gacgccttcg acgcgcgcac ggagcgcgag gtgcgcgtgg tcgccgactg
     4141 gctggccgcg aagtacgagg cgcgccggcg cgcgaacgcg ggcgcgtagg cgcgcgcgaa
     4201 ggggagccgc atgcccaaga agtcctccgg ggtccaactc gaatccgtcg acgcgctgcg
     4261 gcgccgcgtc gaggagctcg agcgctggtt ccagacccac gaccgccagg tccgcttcct
     4321 cgaacgcgag cgccagaagc tctccgcgct cgtcaatcac accgacgccg gattcgtcgt
     4381 cttcgacccc agcctgcgcg tgcgctggct caacaacatc tgccgcgcct ggttcggcgg
     4441 gatgcagagc gcacacgcgg ggcgcacggc ctcgtgcaac gagctgttgt gcggcgagca
     4501 cgcgatctgc gccgcgtgcc cgacggcgca agtgctcgcg accggcaccg tgtcgcaccg
     4561 cgaggttcgc ctcgacgtcg acggcgtcac ccgccacatc tacgccaccg cgatgcccat
     4621 taagtcgcac gacaaccgcg ttgacgaagc catcctgatg ctccaggaca tcaccgacct
     4681 cgaggtgctg cgccgctcgg aagaggcgtc gatgcgcgcc aaagaggccg ccgaatcggc
     4741 cagccgcgcc aagagcgagt tcctcgccaa catgagccac gaggtgcgta cgcccatgac
     4801 cggcgtcctc ggcatgaccg ggctgttgct cgacaccgag ctcacgccgg agcagcggga
     4861 ctacgtgcgc atcgtgcaga gttcggccga agcgctgctc gcgatcatca acgatattct
     4921 cgacttctct cgcatcgagg cgggccgtct gaccatcgac ccgatcccgt tcgacttggt
     4981 ggccgcgacc gacgaagtgg tagcgctcat cgcggagcgc gcggaggaga agggaatcga
     5041 actcatcgtg cgctacgcgc ccgacgtgcc ccgccgcgtg gtgggcgacg cgggccgcat
     5101 ccggcaggtg ctgatgaacc tcgcgggcaa cgcgctcaaa ttcacccacg agggctacgt
     5161 gctcatcagc attgagaagc tggacggtga cgacgagtcg gcgcgcgtgc gcgtctccat
     5221 tgaggacacc ggcatcggca tcgaacacga caagctggac gccgtcttcg agaagttcac
     5281 gcaggccgac ggctcgatca cgcggcgcta cggtggtacc ggcctgggac tcgcgatcac
     5341 gaaggagctg atcgagctca tggacggcga gatcacggtg accagcacgc ccaagagcgg
     5401 ctcgaccttc gccttcacgc tgaccttccc gctcgatcgc gaggccccca tggagcctcc
     5461 gcagcccgag aacctcgaag gcgtgcgcgt gctggtgctg caccgtcacg agaaggtacg
     5521 cggcgtattg cacgagcaag tcgtgagctg gaaggcgcgc gtcaccgcgg tggcgaccgg
     5581 agcggaagcc atcgccgagt tggaggccgc gtaccggc
//
LOCUS       DASPGK010000221         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1345154, whole genome shotgun sequence.
ACCESSION   DASPGK010000221 DASPGK010000000
VERSION     DASPGK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345154"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VEC56_06090"
     CDS             <1..553
                     /locus_tag="VEC56_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13758.1"
                     /translation="AESADFHYREAMRYRGIVHNDPFFGLVIYRMIDEYAHFLDRHGT
                     EADALRQHRVAARVLGTINERLDVYSGSPLAYTDVVAGLAHEAAHIAPFDSAGADTLF
                     TLAMEISGKRMGIDHELTGRVEFALRSLRRGAIGPLDIEDDPYARLPWTLSKRNAMWG
                     DNPWPTTGAVPVAGATTRTDPTN"
     gene            complement(743..>3184)
                     /locus_tag="VEC56_06095"
     CDS             complement(743..>3184)
                     /locus_tag="VEC56_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV13759.1"
                     /translation="YKVGDIARIVKSAGGNLNAGTSYSYTMYYVVLPSREFETALEVQ
                     ADAMMHSTFDTHEFTKERLVVIDEARMYDDRPESFTFYRTMELGFEKHTYRRPIAGYQ
                     HIVEKITRDQLVEFYRNYYRPSNAVLVVVGDVDTDQAFKRIEEAYGGWSNGRVVVNEP
                     PVEPPQTALRFKAYEGPMDHAYLGAGFHVPSILHDDYPALQMLAELASSGKSSRFYRK
                     VREEKRLVTSAGADLLAERWPGFFLLAASMPPAKWESACGALLQEIERFKQDLVSESE
                     LDKARRQVERAMYRELETMEGQASNIGYYQLLGDFRLADRHREAIQRVTPDEIVDVAR
                     KYFHPANLSLVAYVPKSFGLESLTYERISGVVDGALSSTGGVTEMPATPASVSPSSVP
                     TGPRASRAREESAVTSFALDNGVRVLLKQRPNVPLVSMLTVFPGGGRFEPEGKSGLGM
                     LTHRVLTKGGARFSGEEIAEKIEGLGGSIDSYSSFDTGGVSVGVLSEYAEEAVEIYRD
                     VLREPKFDVGRIEQEKSRLLEELARRHDNPVPFAMDHLFRSVFGAHPYAYPFLGDAKE
                     VGALGARDCTEWYHALLAPSRAVLAMVGDLTIERAQSIADRLLGDLAAGPMIEPRADA
                     AGRPFSPGEHVLRRAGIKQAVTFVGFPAARMETPESTALEVANAVLTGLGGRLFVELR
                     DKRSLGYMTGSAYNALYRSGIFFGYANPGAEGIDEAVRVILHELDVISREPVSDEEMS
                     RAKEWLAGSHVMELQRNGAQAVAYGTYEALGFGWPAVDRLPDEIRAVTTDQVMAAARA
                     TFQRENAVIVRMLPE"
BASE COUNT          526 a         1092 c         1011 g          555 t
ORIGIN      
        1 cgccgaatcg gcggacttcc attatcgcga ggccatgcgc taccggggga tcgtccacaa
       61 cgaccccttc ttcgggctcg tcatctatcg aatgatcgac gagtacgcgc atttcctcga
      121 ccgccacggg accgaagcgg acgcgcttcg gcagcaccgg gtcgcggcgc gcgttcttgg
      181 gaccatcaat gaacgactgg acgtgtactc cggatccccg ctcgcgtaca cggacgtcgt
      241 cgccggtctg gcgcacgaag ccgcccatat cgcgcccttc gactcggcgg gcgcggatac
      301 gttgttcacc ctggcgatgg aaatctctgg gaagcgaatg ggcatcgacc acgaactcac
      361 ggggcgcgtc gagttcgccc tccgttcgtt gcggcgcggc gcgatcggtc ccctggatat
      421 cgaagacgat ccttatgcac gcctcccgtg gacgctttcc aagcgcaacg cgatgtgggg
      481 cgacaacccc tggccgacca ccggcgccgt ccccgtcgcg ggcgcgacta cccgaaccga
      541 ccccactaat tagccgcttt ttctcttacc cctccggccg gggttcaccg ctttcgtata
      601 taccgggtgg ttggttacgc cctccgctcg gtgagaatcg ctagggccgg gctcacgtgc
      661 gattaggctg ctatccgcac gggggaacgg aactcgttgc agcaccgggg cgaaagcccc
      721 ggtgctgtaa acgctttgtg ggttactcgg gcagcatcct cacgatgacc gcgttctcgc
      781 gctggaacgt cgcgcgggcg gcggccatga cctggtcggt ggtcaccgca cgaatctcgt
      841 cgggcaggcg gtccaccgcc ggccacccga agcccagtgc ttcgtaggtt ccgtacgcga
      901 ccgcttgcgc gccgttgcgc tgcagctcca tcacgtgcga tcccgcgagc cactccttgg
      961 cgcgcgacat ctcttcgtcg ctcaccggct cgcgcgagat cacatccagc tcgtgcagga
     1021 tgacgcgcac cgcttcgtcg attccctccg cgcccgggtt ggcgtagccg aagaagatcc
     1081 cgctccgata gagcgcgttg taggccgacc ccgtcatgta ccccagcgag cgtttgtcgc
     1141 gcagctcgac gaagagccgc ccgcccaggc cggtcaaaac cgcgttcgcg acttcgagcg
     1201 cagtcgattc gggcgtctcc atgcgcgcgg ccgggaatcc cacgaaggtc accgcctgct
     1261 tgatgcccgc gcgtcgcagc acgtgctcgc cgggggaaaa cggccggccc gccgcatcgg
     1321 cgcgcggctc gatcatcggg ccggccgcca aatcgcccag caatcgatcc gcaatgctct
     1381 gtgcgcgctc gatggtgaga tcgccgacca tggcgaggac ggcacgcgac ggcgccaaga
     1441 gcgcgtgata ccactcggtg cagtcgcgcg cgcccagcgc gccgacttcc ttggcgtcgc
     1501 ccaggaaggg gtacgcgtac gggtgcgcgc cgaagaccga gcggaacaag tggtccatcg
     1561 cgaaaggcac cgggttgtcg tggcgacgcg cgagctcttc gagcaggcgt gatttctcct
     1621 gctcgatgcg cccgacgtcg aacttgggct cgcgcagcac gtcgcggtag atctcgaccg
     1681 cctcttcggc gtactccgag agcaccccga ccgacacgcc gccggtgtcg aacgaggagt
     1741 aactgtcgat cgaaccgccc aggccctcga tcttctccgc gatctcctcc ccggagaagc
     1801 gcgcgccgcc cttggtcagc acgcggtgcg tcagcatccc caaccccgac ttcccttccg
     1861 gctcgaagcg cccgccgccc gggaacaccg tgagcatgga taccagcggg acgttgggtc
     1921 tttgtttcaa taagacgcgc acgccgttgt cgagcgcgaa cgacgtgact gcggattcct
     1981 cacgcgcacg cgaagcgcgc ggcccggtcg ggaccgacga cggagatacg gacgcgggtg
     2041 tcgcgggcat ctccgtcacg ccaccggtcg agctcagggc cccgtcgacc acgcccgaaa
     2101 tacgctcgta ggtcaggctc tccagaccga aggacttggg gacgtacgcg accagcgaaa
     2161 ggttcgcggg gtggaagtac ttgcgcgcga cgtccacgat ctcgtcgggt gtgacgcgct
     2221 ggatcgcttc gcgatggcgg tcggcgagcc gaaaatcgcc gagcagctgg tagtagccga
     2281 tgttcgatgc ctgtccctcc atagtttcca actcgcggta catcgcccgc tccacttgcc
     2341 ggcgcgcctt gtccaattcg gactccgata ccaggtcctg cttgaagcgc tcgatctcct
     2401 gcaagagcgc gccgcacgcc gactcccact tggcgggcgg catcgacgcg gccaggagga
     2461 aaaacccggg ccatcgttcg gccagcaaat ccgcgcccgc gctggtgacc agacgcttct
     2521 cttcgcgcac cttgcgatag aagcgcgaac tcttacccga gctcgcgagc tccgcgagca
     2581 tctgcagcgc cggataatcg tcgtgcagga tgctggggac gtggaagccc gcgcccaggt
     2641 acgcgtggtc catggggccc tcgtaggcct tgaagcggag tgccgtctgg gggggctcga
     2701 cgggcggctc gttgacgacg acgcgcccgt tggaccaccc gccgtacgcc tcctcgatgc
     2761 gcttgaaggc ctggtcggtg tcgacgtcgc ccaccaccac cagcaccgcg ttcgacggcc
     2821 ggtagtagtt gcggtagaac tccaccaact ggtcgcgcgt gatcttctcg acgatgtgct
     2881 ggtagccggc aatcgggcgc cggtaggtgt gcttctcgaa gcccagctcc atggtgcgat
     2941 agaacgtgaa cgactccggg cggtcgtcgt acatgcgggc ctcgtcgatg acgaccagtc
     3001 gttctttcgt gaattcatgg gtgtcgaagg tactgtgcat catcgcgtcg gcctgcacct
     3061 cgagcgcggt ctcgaattca cgcgacggca gcacgacgta gtacatcgtg tacgagtagc
     3121 tcgtgcccgc gttgagattg ccgcccgccg acttgacgat acgcgcgatg tcgccgacct
     3181 tgta
//
LOCUS       DASPGK010000222         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_174885, whole genome shotgun sequence.
ACCESSION   DASPGK010000222 DASPGK010000000
VERSION     DASPGK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174885"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..2900)
                     /locus_tag="VEC56_06100"
     CDS             complement(297..2900)
                     /locus_tag="VEC56_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13760.1"
                     /translation="MLLLPSDGLARNPIRNAFFSVYPSAVGTRLDNLPSNSGHCGACH
                     FDFDGGGPRNPYGSAIEVKINQGLSATNAILAVAGDDVDNDGYSSLIEITSTSFGNTP
                     TFPGLKAGDESSVINVSPLDLIGYLTPTGGTDTTPPVVAVIWPNGGQVFAANSTQSIQ
                     WSATDAGGISLVSLYVSDDGGVTFTPISFNEANDGSFDWFVPNRPGSAYIARVIARDN
                     AGNYGSDWGDGLFTITVAPGGIAPTTLRDMDLPGTQPFALALADPQDCAGCHADYDLN
                     AEPWHQWKGSMMAHAQRDPLFLACLAVAEQDAPSVGDLCLRCHTPGGWVEGRSVDTGG
                     GLVNAKDRQSIHCDFCHHLVDPVYVAGVSPVQDVAVLDSLDAVPPGYANGQFVLDPNA
                     IRRGPFADAQASHAFLESPFHRSSNLCGTCHDVSNPVFEYAGAPGKYAPGAFDTPHPD
                     GNVRNMFPVERTFSEWTQSQYANGGVFAPQFAGNKPDGIVSTCQDCHMQDVSGKGCNE
                     PGAPTRANLPKHDLTGGNTFLPDILPVFFPGEVNVAELQDGKQRAIAMLQLAASMSLA
                     AGQDGPHPTVTVTVTNETAHKLPSGYPEGRRVWLNVKAYDESDALVYESGAYDPATGI
                     LTHDADAKIYEIKIGVSSRLAPILNLPAGPSFHFVLNDTVYSDNRIPPRGFTNAAFEA
                     VQSPAVGHAYADGQHWDETMYVLPTEARFVRATLYYQTTSKEYIEFLRDANTTNSAGI
                     DLYNAWVAQGRGAPVAMTTDTISVDLQPTDVSAPLATTELYPNYPNPFNPATSIRFSL
                     SKRQRVRIEVFDVTGASVKKLVDEERPAGVQRVEWNGRNASGEPIASGVYLVKMQTRE
                     GNFVRKAVLLK"
BASE COUNT          513 a          977 c         1003 g          570 t
ORIGIN      
        1 cacgatttgc tcgtgcgggt ggtcgtgctc ggggatcgac gaaccggcgt cgatgcgcca
       61 gtgcgcgacc gtcatgcgcg ccgaatgcac gaagcgcgcg agatgcccgg gttgcacctc
      121 gcgcggttcc acgtccttta gattgatgag ctgcattcaa gaacctcctg cgtgcgtgat
      181 cggagaatgg tccgaatggc agtataccgc gtctatcgcg atcgcgagcc ggcaagaaac
      241 gcaaagggcg cgagtcgatt gacccgcgcc ctctaccccg gctcgcttac aatcgattac
      301 ttcaacagga ccgccttgcg cacgaagttg ccctcccgcg tctgcatctt gaccagatac
      361 acaccggacg cgatcggttc gccggacgcg ttccgcccgt tccactccac gcgttgaacg
      421 cccgccggcc gctcttcgtc gaccagcttc ttgacgctcg caccggtgac gtcgaagact
      481 tcgatgcgca cgcgctgccg cttcgacagc gagaagcgaa tcgaggtcgc cgggttgaac
      541 gggttggggt agttcggata gagctcggtg gtcgcgagcg gagcactcac atccgtgggc
      601 tgcaggtcga cgctgatggt gtcggtcgtc atcgcgaccg gcgccccgcg tccctgcgcg
      661 acccacgcat tgtacaggtc gatgcccgcg ctgttggtgg tgttggcatc ccgcaggaac
      721 tcgatgtact ccttggaggt cgtctggtaa tacagcgttg ctcttacgaa gcgcgcctcc
      781 gtgggaagca cgtacatcgt ttcatcccag tgctgaccgt ccgcatacgc gtgtcccacc
      841 gccggcgact gaaccgcctc aaaagccgcg ttggtgaagc ctcggggtgg gatgcgattg
      901 tcggagtaca ccgtgtcgtt caggacgaaa tggaacgacg gtcctgcggg gagattcaag
      961 atcggcgcca agcgcgagga aacgccgatt ttgatctcgt agatcttcgc gtcggcgtcg
     1021 tgcgtcagta tcccggtggc cggatcatag gcgcccgact catacaccag cgcatccgat
     1081 tcgtcgtacg ccttcacgtt gagccatacg cgacgcccct ccggatatcc cgacggcagt
     1141 ttgtgagccg tctcgttcgt caccgtgacg gtcacggtgg gatgggggcc gtcctggccg
     1201 gccgccagcg acatcgacgc cgccagttgc agcattgcga tcgcgcgctg cttcccgtcc
     1261 tgcaactccg ccacgttgac ttcgcccgga aagaagaccg gcaggatatc gggcaggaac
     1321 gtgttcccgc cggtcaggtc gtgcttgggc agattggcgc gcgtgggcgc gccgggctcg
     1381 ttgcagccct tcccgctcac gtcctgcatg tggcagtcct ggcacgtgga cacgatgccg
     1441 tcgggcttgt tgcccgcgaa ctggggcgcg aacacgccgc cgttcgcgta ctggctctgc
     1501 gtccactcgc tgaacgtgcg ctcgacgggg aacatgttgc gtacatttcc gtcggggtgc
     1561 ggagtgtcga acgcaccggg cgcgtacttg ccgggagctc cggcgtactc gaagaccggg
     1621 ttgctcacat cgtggcaggt cccgcacagg ttggacgagc ggtgaaacgg cgactcgagg
     1681 aacgcgtggc tggcctgcgc gtccgcgaac ggaccgcggc gaatcgcatt gggatccaga
     1741 acgaactgcc cgttcgcgta tcccggcggc accgcatcca gcgaatcgag aaccgcgacg
     1801 tcctgcacgg ggcttacccc ggcgacgtag acgggatcga ccaagtggtg gcaaaaatcg
     1861 cagtggatgc tctgccggtc cttcgcgttg accagaccgc cgccggtgtc gacggagcgt
     1921 ccttcgaccc agccgccggg tgtgtggcag cgcaggcaca agtcgccgac cgacggcgcg
     1981 tcctgctccg caaccgcgag gcacgccagg aagagggggt cgcgctgcgc gtgcgccatc
     2041 atgcttccct tccactggtg ccacggctcc gcgttgaggt cgtaatcggc atgacagccc
     2101 gcgcaatcct ggggatcggc gagcgcgagc gcgaacggct gcgtgcccgg caggtccatg
     2161 tcgcgcagcg tggtcggcgc gatgcctccg ggcgcgacgg tgatcgtgaa cagcccgtcg
     2221 ccccagtcgg agccgtagtt gcccgcgttg tcgcgcgcga tcacgcgcgc aatgtaggcg
     2281 cttcccgggc gattgggcac gaaccagtcg aacgagccgt cgttcgcttc gttgaacgaa
     2341 atgggcgtga acgtcactcc gccgtcgtcg gaaacgtaca ggctcacgag cgaaatcccg
     2401 ccggcgtccg tcgccgacca ctggatgctc tgcgtcgagt tggcggcgaa gacttgcccg
     2461 ccgttgggcc aaattaccgc gacgaccggc ggcgtggtgt ccgttccgcc cgtgggcgtc
     2521 agatagccga tgagatcgag gggactgacg ttgatcaccg acgattcgtc gcccgccttc
     2581 agcccgggaa acgtcggcgt gttcccgaag ctggtcgacg tgatctcgat caggctgctg
     2641 taaccgtcgt tgtcgacgtc gtccccggcg accgcgagaa tcgcattggt ggcggaaagt
     2701 ccctggttga tcttgacctc gatcgcgctc ccatagggat tgcgcgggcc gccgccgtcg
     2761 aaatcgaagt ggcaagcgcc gcagtgcccg ctgttactgg ggagattgtc gaggcgcgta
     2821 ccgaccgcgg acggatatac ggagaagaat gcgttgcgaa tggggttacg agccaggccg
     2881 tcgctgggca ggagcagcac tccaatcgcg aaagagaatc ctattctgca tgttgtcttc
     2941 acggcctcac ctctcttctg gagatcgaat gggccggtcg ccctgcggcg ccggcccatt
     3001 cgcatgcgtc aacgagagtg gccgcatttt cctcccgccg acgacttacg gttctattgc
     3061 ttt
//
LOCUS       DASPGK010000223         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_878451, whole genome shotgun sequence.
ACCESSION   DASPGK010000223 DASPGK010000000
VERSION     DASPGK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878451"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1688)
                     /gene="nadB"
                     /locus_tag="VEC56_06105"
     CDS             complement(102..1688)
                     /gene="nadB"
                     /locus_tag="VEC56_06105"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXV13761.1"
                     /translation="MVQTDFLIIGSGIAGLSLALNLAERGRVTVVTKKALEDSNTNYA
                     QGGVAGVLGSDDSFELHVEDTLGAGAGLCRREIVEMVVREGPARIRDLTSLGVAFTTQ
                     GGRLALGLEGGHSRHRIVHHADHTGREIESHLLDAVLEHERIDVHPYHMAIDLIAPRH
                     LSRGDENGVWGAYAMDAGTGEVRAFVAKRTILATGGAGKVYRYTTNPDIATGDGVAMA
                     YRVGARIANLEFVQFHPTCLYHPRAKSFLLTEALRGEGGRLIDATGARFMDAYDPRGE
                     LAPRDIVARAIDSEMKRTAAPCVYLDIAHFSPALIERRFPSILGRCLELGIDPRRVPL
                     PVVPATHYFCGGVDVDARGRTSVPNLFAVGEVSHTGLHGANRLASNSLLEALVFAERA
                     HTAVLEGVEQTSLPDVKRWIAPGTGAAPDPVVLEHDWDEARRVMWDYVGIVRSDARLA
                     IAAERMASLATTVEAVYRASRVTLDLLELRNIVQVGRLVVESAQSRKESRGLHFTESY
                     PARDDAHFLRDTVVVARAEH"
     gene            1991..2539
                     /locus_tag="VEC56_06110"
     CDS             1991..2539
                     /locus_tag="VEC56_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13762.1"
                     /translation="MAKRYIALVAVLAVMALVACDDAPESGRTIVSIQDFNLGTPVQS
                     DVVVDNGTDPAYISEDLIPVTFTARPYNAFITGTTHNQVVIESYHVVWTRTDGGTGTL
                     ATRDEASSIYVSVGDETEAFIRLVTWGDKAGPVLSPLIGSANQISMRADVTFTGREVG
                     TEEEIELSASVSVNFSDAVNIE"
     gene            complement(2613..3671)
                     /gene="lpxK"
                     /locus_tag="VEC56_06115"
     CDS             complement(2613..3671)
                     /gene="lpxK"
                     /locus_tag="VEC56_06115"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXV13763.1"
                     /translation="MRTISSNRSTWEGVPALTPLSWVYRGGVAAARALRRPAGAGDST
                     PRVVAIGNLEVGGSGKTPLALWLLERWARAGKRAAYVSRGFGSAAGTGPLVTVVPAAN
                     AAPASFAGLRLVFRDGPDLAASIGDEAAMVARRATAIPLVIARDKRRAVEVASAMGAE
                     VVVVDDAFQSFALARHLDLLLLDARRPLASGRVLPAGRLREPPSAIARADVVVFNGAA
                     DAAAIEGATARVARWIHARQRVYGLRRVVRLVAATPSAKEPVSRAVVVSGIARPDDLR
                     DAIEALGVHVADAIAFRDHHRYTEADVGVIRERARGGAIVTTEKDWVKLERFEWGETP
                     VWVARLDVELVGGGEFDA"
     gene            complement(3679..4320)
                     /locus_tag="VEC56_06120"
     CDS             complement(3679..4320)
                     /locus_tag="VEC56_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV13764.1"
                     /translation="MKASLVLLPPLGALVIRALGTTWRYSVRGRDHVEAARSHGQLVY
                     AFWHGRLLPLAYLHRGDAARVLASEHHDGELLGRTVRYLGLGYVRGSSTRGGTKAILA
                     LADAVRAGYDVALTVDGPRGPRFEVKPGVVEVAKLTGGAIVPVSTGSRRRRVFASWDA
                     FELPAPFSRVVVEYGPAIRVRPDAGREEMEGKRRELEESLRRLTESCDREAHA"
     gene            complement(4317..>4648)
                     /locus_tag="VEC56_06125"
     CDS             complement(4317..>4648)
                     /locus_tag="VEC56_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXV13765.1"
                     /translation="LQSALLETPLTIVYRTTSFNYALARRLVTIDRVGLVNVLVGSEV
                     APEFVQGRAQPEQIARATAALLEDSARRAATIARFRELRASLAGGSGAERVAEIAIDL
                     VAGGRTA"
BASE COUNT          736 a         1666 c         1548 g          698 t
ORIGIN      
        1 cggggccagt ccgcgcgcga cccgcaccgc ttcctggtcg tcgcggtgcg ggtaaaccag
       61 cggatagatg tcgccaaagg cgaccttgta ccactccatg gtcagtgctc cgcgcgcgcg
      121 accaccaccg tgtcgcgcaa gaagtgcgcg tcgtcgcgcg ccgggtagct ctccgtgaag
      181 tgcaggccgc ggctctcctt gcggctctgc gccgattcca ccaccaagcg tcccacctgg
      241 acgatgttgc gcagctcgag cagatcgagt gtgacgcggc tggcgcgata gaccgcctcg
      301 accgtggtcg cgagcgacgc catgcgctcg gcggcgatcg cgaggcgcgc gtcgctgcgc
      361 acgataccga cgtagtccca catgacgcga cgcgcttcgt cccagtcgtg ctcgagcact
      421 accgggtcgg gcgcggcacc ggtgccgggt gcaatccatc gcttcacgtc gggcagactc
      481 gtctgctcga ctccctccag caccgcggtg tgcgcgcgct cggcgaacac gagcgcctcc
      541 aagagcgagt tgctggcgag tcggtttgcg ccgtgcaagc cggtgtggct cacttcgccg
      601 acggcgaaca ggttgggcac cgacgtgcgt ccgcgcgcgt cgacgtccac cccgccgcag
      661 aagtagtgcg tcgccggcac caccggcagc gggacgcggc gcgggtcgat gccgagctcg
      721 aggcagcgcc cgaggatact gggaaagcgt cgttcgatga gcgcgggcga aaaatgcgcg
      781 atgtcgaggt acacgcaggg cgcggccgtc ctcttcatct cgctgtcgat ggcgcgggcc
      841 acgatgtcgc gcggggccag ctcgccgcgc gggtcgtacg cgtccatgaa gcgcgcaccc
      901 gtggcgtcga tcaggcgccc gccttcgccg cgcagcgctt cggtcaggag gaacgacttg
      961 gcgcgcgggt gatacaggca cgtgggatgg aactgcacga actcgagatt cgcgatgcgc
     1021 gcccccacgc ggtacgccat ggccacgccg tcgccggtcg cgatgtccgg attggtggtg
     1081 tagcggtaga ccttgccggc gccgccggtg gcgagaatgg ttcgcttggc gacgaacgcg
     1141 cgcacctcgc cggtgcccgc gtccatcgcg tacgcccccc acacgccgtt ttcatccccg
     1201 cgcgagaggt ggcgcggcgc gatcagatcg atcgccatgt ggtaggggtg cacgtcgatg
     1261 cgctcgtgtt cgagcacggc gtccaacaaa tgggattcga tctcgcggcc ggtatggtcg
     1321 gcgtggtgga cgatgcgatg gcgcgagtgc cccccctcga gtcccagcgc gaggcgcccc
     1381 ccttgagtgg tgaaggcgac ccccagcgag gtgaggtcgc ggatgcgcgc gggcccttcg
     1441 cgcacgacca tctcgacgat ctcgcgccgg cacaaaccgg cgcccgcgcc gagggtgtct
     1501 tcgacgtgga gctcgaagct gtcgtcgctg cccagaacgc cggccacacc gccctgcgcg
     1561 tagttcgtgt tagagtcctc aagggccttt ttggtcacga cggtcacccg cccccgctcg
     1621 gcgaggttca acgcgagcga gaggccggcg attcccgagc cgatgatgag gaagtcggtt
     1681 tggaccacgg aacgtcttaa gtcgaagttc ttccttggga tgcgggcagt gtacccgatg
     1741 ctaggcaggg cgcgccccga tttgcaacaa cgatcccctg caacacgcag attgcacatc
     1801 ggcgaaccgg gtcggcgctc ggcggcaaac ccagtcggca cgctaaggcg tttacatcac
     1861 gcgggttccg ccggcggtgc ggtggtggtc gctggcacga cgattgcgaa tgacccccac
     1921 cgattgccca ttccgccgac cccgaagaga gcggcggcgg tggcgagcac ccttttagga
     1981 ggtaggtttc atggcgaagc gttacatcgc gttggtcgcc gtcctcgccg tgatggcgct
     2041 ggtcgcgtgc gacgacgcgc ccgaatcggg acgtaccatc gtgagcattc aggacttcaa
     2101 cctcggcacg cccgtccaga gcgacgtcgt ggtcgataac ggaaccgacc cggcgtacat
     2161 ctccgaggac ctcattccgg tgacgttcac ggcgcgccca tacaacgcgt tcatcaccgg
     2221 cacgacccac aaccaggttg tcatcgagag ctaccatgtg gtgtggactc gtaccgacgg
     2281 cggcaccggc accctggcga cccgcgacga ggcctcgtcg atctacgtgt cggtgggcga
     2341 cgagacggaa gcgttcattc gtctggtcac ctggggcgac aaggcgggtc cggttctatc
     2401 gccgctcatc ggctcggcga accagatcag catgcgcgcc gacgtgacct tcaccggccg
     2461 cgaggtcgga accgaggaag agatcgagct cagcgcctcg gtgtcggtca acttctccga
     2521 cgccgtcaat atcgagtaac ggtcggtatc ggcctacctc ccgattcgaa acgagcgccg
     2581 cagagcagac tctgcggcgc tcgttgttgt cgctaggcgt cgaactcccc accgcccacc
     2641 aactcgacgt ccaggcgcgc gacccagacc ggtgtttctc cccattcgaa gcgctcgagc
     2701 ttcacccagt ccttttcggt cgtgacgatc gctccaccac gcgcgcgctc gcggatgacc
     2761 ccaacgtcgg cctccgtgta gcgatggtgg tcgcggaacg cgatcgcgtc ggccacgtgc
     2821 acgcccaagg cttcgatcgc atcgcgcagg tcgtccggcc gcgcaatgcc cgaaacgacc
     2881 acggccctcg acaccggctc tttcgccgaa ggagtcgcgg caaccagtcg aacgacgcgc
     2941 cgcagtccgt acacgcgctg gcgcgcgtga atccagcgcg cgacgcgtgc cgtggcaccc
     3001 tcgatcgcgg ccgcatcggc cgcgccgttg aagaccacca cgtcggcgcg cgcgatcgcc
     3061 gacggaggct ctcgcaaccg cccggcggga agcacgcgcc cgctggcgag cggccggcgg
     3121 gcatcgagca ggagtagatc cagatgacgc gcgagggcga acgattggaa cgcgtcgtcg
     3181 acgaccacga cctcggcgcc catggcggaa gcgacctcca ccgcgcggcg cttgtcgcgc
     3241 gctatcacga gcggtatagc ggtcgcgcgc cgcgcgacca tcgccgcctc gtcgccgatc
     3301 gacgcggcca ggtcgggccc gtcccgaaac accagccgca accccgcgaa cgaggccggg
     3361 gccgcgttcg ccgcgggtac cacggtcacc agcggtccgg tccccgccgc acttccgaag
     3421 ccgcggctca cgtacgcggc tcgcttgccc gcgcgcgccc accgctccag gagccacaac
     3481 gcgagcggcg tcttcccgct tccccccact tcgagattgc cgatcgcgac tacccgcggc
     3541 gtcgagtctc ctgcgcccgc gggtctgcgc agcgcgcgcg ccgccgccac cccgcctcga
     3601 tacacccacg acagcggcgt gagcgcgggc acaccttccc acgtacttcg gtttgagctg
     3661 atggtgcgca tggttgagct acgcgtgcgc ctcgcgatcg cacgactcgg tgagtcttcg
     3721 cagactctcc tccagctcgc gtcgcttccc ttccatttcc tcgcgaccgg cgtcgggtcg
     3781 gacccggatg gccgggccgt actcgaccac gacgcgcgag aacggcgcgg ggagctcgaa
     3841 ggcatcccac gaggcgaaca cgcggcgccg ccgcgatccc gtcgataccg gcacgatggc
     3901 gcccccggtc agcttggcca cttcgaccac gcccggcttc acctcgaagc gcgggccgcg
     3961 cggcccgtcg acggtgaggg cgacgtcgta gcccgcgcgc acggcgtcgg ccagggccag
     4021 gatcgccttg gtcccgccgc gtgtgctcga tccccgcacg taaccgagtc ccagatagcg
     4081 gacggttcgt cccagcagtt cgccgtcgtg gtgttcgctc gcgagcacgc gcgccgcatc
     4141 gccgcgatgg agatacgcga gcgggaggag tcggccgtgc cagaacgcgt acaccagttg
     4201 tccgtgcgag cgcgccgcct cgacgtgatc cctcccgcgc acgctataac gccacgtcgt
     4261 gcccagcgcg cggatgacca gggcgcccag gggcgggagc aggacgagcg acgccttcac
     4321 gccgtcctcc cgcccgcgac caggtcgatc gcgatctccg ccacgcgctc cgcacccgac
     4381 ccgcccgcga gactcgcgcg cagctcgcga aaacgagcga tggtggcggc gcgccgcgcc
     4441 gaatcctcca gcagggcagc ggtcgcgcgc gcgatctgtt ccggctgcgc gcgcccctgc
     4501 acgaactcgg gcgccacttc cgagcccacg agaacgttca ccaagccgac gcgatcgatg
     4561 gtcacgaggc ggcgcgccag cgcatagttg aaactggtgg tgcgatacac gatcgtgagc
     4621 ggcgtctcca gcagcgccga ctgcaggg
//
LOCUS       DASPGK010000224        10611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_761084, whole genome shotgun sequence.
ACCESSION   DASPGK010000224 DASPGK010000000
VERSION     DASPGK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761084"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /gene="bamA"
                     /locus_tag="VEC56_06130"
     CDS             <1..1873
                     /gene="bamA"
                     /locus_tag="VEC56_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXV13766.1"
                     /translation="VRAQGSDPPPDDRVVFVNRIAIVGNQSVETAVLRARMRTKEPSF
                     FSILRKPKFDREQIERDAAQLEAYYHAIGYPDATVRIERVDYLENGRFADVTIRVVEG
                     EPIRVATVSFPHPLVVDEAELRKKLLLAPGKPYNASLLQTDVYRIKTEHYNLGYLGVL
                     VIDSTTVIGRQVDIRFIVDPGLPLNVGAIVVEGNRLVRRSVIDAEIELRSGDVCRFEK
                     VVKTQRNLFETGLFNVVDVLPENVDPVRQTVDIRIRVRERKQSWIETGFGVGNVLGSR
                     IFAEWGTRNLFGTGRTLRFKAQYAFDLFAGDDIDFNQIDISNTYYRYDAVYQQRRIFG
                     IKLGAGVNAFIEEDRTVPNLEVKTQGTTFGVAHNFGRPRRDFGYDTEVVGTFSIENIT
                     RREFGLPEEESRSNILGGSLSRDRRDFVLNPRRGEYRVVTAAVAGGILGGKNDYYAIT
                     GNEQRYHGRGASVLAWRARVGYSAAYGRSDRVPVEDRFYLGGANSVRGYDEASLGPRS
                     VESGESVVDGGEFMALANVEFRYPLPLLGRWNFAGTFFLDGGNVWGSASDVTASDFRL
                     SSDVSETEVDDFRYGIGLGVRYNTPIGPIRVDWGYPLKPDVYTDESGTFYLSLGQIF"
     gene            1877..5776
                     /locus_tag="VEC56_06135"
     CDS             1877..5776
                     /locus_tag="VEC56_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXV13767.1"
                     /translation="MANSDQQIRPERRRSNVRRISAVIAVVISIALAVVAYRESRILP
                     ERIAGYVNRHYLAGTPFEFSLDGVSGTFVRRINLQNPVLRYHSPEASYNVFRADRISV
                     TYELIPVFAFRLLVDDVELENVVVHLRQDQEGRLIIPGTGVPKPGAKKKAVSPVVDVR
                     HFRIDGLALTFGGNETQLAIRDVNLDGAFAYDRGVGHLSIEEGRAYLLDTGTTVEAVR
                     LNARSDWRSIFVDDFAVKLDSSFVVAKGEFREGRFQDVELVLNPISLEELHQLGLIPD
                     EHGTFDARLSLSGTVDSLGVRGRVSGEGLGVALENVDVEGHVTPRALDLDRLAGKVFG
                     SSVDGAFRIEIESEDFVFDGRVADLDLGRGFIEDSDLPPMSLNGAVRVEHTKAVGRYV
                     WRGELERGVIDGYETFDVSAAGVWVDGVGLTLDRFSTVRPSYRIEGSGTVADQGPANV
                     VFKAEGTDFSYFWNYFELPPVSGAMALTGHLEGPLDDFQVNVNGTVRDLAWEFTEVDS
                     GTVQAEVRRAGTLAPEVTLSLSGRRARVYGHPVVNPSLLMDVDTTSVRIHDVRVTRSD
                     STFVADFDVVARGKESRVFLKHAALETPNESWNTTGPSVIDVSEDAVVVDSLVLASSR
                     GEFGYSGTRRVREKRIDGHVWGRNVNLAIFRDVLRVPIAIEGVGTFDLHLEGSEENPR
                     ARLSAAITRGLVDSVAFDAARATLSFDGERYRLSELWVQEGDDTLTAQGEWACDLAPR
                     VLAREDRPASLWRAPISVRARVGQYDLSSLFRAMHRPTDVASTYTGTVTVGGTLGDPE
                     LHTRGEFMPLPGPGHPIPPTQVDAAYRNGALRIARLDISQVVNARATATIPMTVSFRD
                     GARIQTDRPMEARIEIDPLANDALSNFTPYVRDVSNLRGVLSGRVDASGTPSSPRLTG
                     ALSFSRGELRVAGVQEGASGIAMRVDFVDDVIRLTSLTASSGKKGSLVGAGWARVSNY
                     RLVDYQVDVTAHEFPLRSIPDVEVVLGGTLTARLSEWSGGGRIPTLTGRLNVKEAVIS
                     KDLSSGTNTGSELTRPTARPDWLASIDVVAPKNVWVRNEDLNVELGAEDLVFLRDERG
                     MYFRGELTVLRGSYKLYGNKFTITQGTMDFSASETLRPSMNIEAYTPHRGGDEVDNRI
                     YLVLSWPYDKLEPHISLSYDEPGYSESDIWRMLGGNIVSGGVATNTLERAINAQMAGG
                     FTVDVDQRPIEEVGAADQSQETLIGVGKYLWEDIYLQYRRGLSVGGEQEFNVEYRLSN
                     KFLLRSQFIYNSRRNRTGIVGQNTDEFNLDLKYRFEY"
     gene            5890..9174
                     /locus_tag="VEC56_06140"
     CDS             5890..9174
                     /locus_tag="VEC56_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV13768.1"
                     /translation="MKLHSTFAAIATGVLLGVLYPATASAQYFGRNKIQYNDYTWHVL
                     STPHFDIHYYEGAEAFAVRAALVLEDGYEEYTYKLKVVLPWKVPVILYSNHADFLETN
                     VTSGQLSEGVQAFAEPSRRRIVLPFTSSFKEFRHTAIHELAHVFTFHIVYNRMLDNVF
                     TRNYLFAMPLWVAEGLAEYLAEGWDADADMFIRDAVIQDYLYPFHLISGFYVYKEGQS
                     VFNYIADTYGHEKVLEILNALAGTRSAEAALQRTLGLNEDQLYEQWSKALRKHYWPLY
                     PDKQELDDVGRRLTDHIKDRAYYNTKPVLSPDGEHIVFFSDRSGMIEVRVISALDGTE
                     VAGVVTDSRTNKYESLHLLTSSVSFDPTGTFVAFVAKSKGHDALFVRNFRTAEERTYE
                     IESNGLTSPAWHPTRNEIVVSATFHGQTDLLTVNLDDGTTKRLTNDPADQLTPRFYPD
                     GSKIVFVHFPEITIPVPPDFDGDNRTALSEMDFLDPNNVKDDANYDIYEFDVATGKQR
                     PLVHSPGDDTEPVILSDGKTLIYASDESGVNNLHAGNVDTGVTYRFTDVLGGLFTPTV
                     HEEKGRIAFSAFVKGGWDIFVSDDLPTMLGRKYAEPARRVLAHDPKVAAKDLEIAQKA
                     AFEAETPAAGDSAQAAALEKSLAAQASTAPTVNGMVEGVADSTAQALADTTAAAADST
                     ASEAFEIDLKDYQPAIRPLDPPAPAESTQSSSIGVRPRLEGVNAPVGADESVFRGATV
                     NRYHTKLAPDFIGSGGGLYFSSGFGFGLSNSVALSDMLGDHHLMFAFSLYQDIANSDV
                     LVAYTYLKRRVDFSFGAYQFSGYYDSRVSSVGETFSTNQLFSERNYGVFGLMSVPFNK
                     FYRMEVDLQAYYSDREFFQDIDPTVDYQYTTVEKSKVRLIEPALAFVHDSSFYGPFGP
                     VDGSRWRISLARGVAFEEQDVSRTTGYIDWRWYRTLFWRNSLALRFSGAASVGEDPRV
                     FFLGGPTRLRGYEWDQIEGSRMWYGSFEYRFPLIDALILGWPGRWGFTNIGATAFFDV
                     GAAWNRGIPDLFVDDRLDQAYSDIGFGLYMYAGYFLLSFQFAWPTDVSTINDDMKFHF
                     YMGPTF"
     gene            9714..>10611
                     /locus_tag="VEC56_06145"
     CDS             9714..>10611
                     /locus_tag="VEC56_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV13769.1"
                     /translation="MSNVTNLNDRQREAVLTTEGPVLVIAGAGSGKTRVLTERIAHLV
                     GDLRVSPYEILAFTFTNKAAREMKDRLERAHPGSTEMLWVGTFHSTGVRILRRHGDSA
                     GVKRDFSIYDTDDSTGLVKAILARNPNDARFVKSPRVLRDRISRMKNDLITPTDAAER
                     AVSAGEQRVAALYAEYERDLRKANALDFDDLINKVVELFATDDRVRELYARRFRYVLV
                     DEFQDTNPIQMAMIDGLASVHGNLFVVGDDDQSIYSWRGARVEHILEFEGLYPATKVI
                     RLEQNYRSTKTILDAANGVIAHN"
BASE COUNT         1837 a         3485 c         3405 g         1884 t
ORIGIN      
        1 ggtccgcgcg cagggttccg accccccgcc ggacgatcgt gtcgtcttcg tcaatcgcat
       61 tgcgatcgtc ggcaaccaaa gcgtggaaac cgccgtgctc cgcgcccgca tgcgcaccaa
      121 ggagccgtcc ttcttctcga tcctgcgcaa gcccaagttc gaccgcgagc agatcgagcg
      181 cgacgcggcc caattggagg cgtactacca cgcgatcggc taccccgatg ccacggtccg
      241 catcgagcgc gtcgactatc tcgagaacgg gcgcttcgcc gacgtcacca ttcgcgtcgt
      301 ggagggcgag ccgattcgcg tcgcgaccgt gagctttccg caccccctgg tcgtcgacga
      361 agccgagctg cgcaagaagc tcttgctcgc gcccggcaaa ccctacaacg cctcgctgct
      421 ccagaccgac gtctaccgga tcaagaccga gcactacaac ctgggctacc tcggtgtgct
      481 ggtgatcgat tccacgaccg tcatcggccg ccaggtcgat attcggttca tcgtcgaccc
      541 cgggctaccg ctcaatgtgg gcgccatcgt cgtggagggt aaccggctgg tgcgacgcag
      601 cgtgatcgac gccgaaatcg agctccggtc gggcgacgtg tgtcgcttcg agaaggtggt
      661 caagacccag cgcaatctct tcgagaccgg gctgttcaac gtcgtcgacg tcttgcccga
      721 gaacgtcgac ccggtccggc agaccgtcga cattcgcatc cgcgtgcgcg agcgcaagca
      781 atcgtggatc gaaaccggct tcggcgtggg caacgtgctc ggtagccgca tcttcgcgga
      841 gtgggggacg cgcaacctct tcggtaccgg acgcaccctg cgcttcaagg cgcagtacgc
      901 gttcgacctg ttcgcgggcg acgacatcga cttcaaccag atcgacatca gcaacaccta
      961 ctaccgctac gacgcggtct accagcagcg gcgcatcttc ggtatcaagc tgggtgccgg
     1021 cgtcaacgcg ttcatcgaag aggaccgcac ggtacccaac ctcgaggtca agacccaggg
     1081 aaccaccttc ggggtggcgc ataactttgg taggccgcgc cgcgacttcg gctacgacac
     1141 ggaagtggtg ggtaccttct cgatcgagaa catcacgcga cgcgagttcg ggcttccgga
     1201 ggaggagtcg cggtccaata ttctgggagg ctccttgagc cgcgacaggc gcgacttcgt
     1261 cctcaatccg aggcgcggcg agtaccgcgt ggtgacggcc gcggtcgcgg gcggaatcct
     1321 gggcggaaag aacgactact acgcgattac gggcaacgag cagcgctatc acgggcgcgg
     1381 cgcttcggtg ctcgcctggc gcgcccgcgt ggggtactcg gcggcgtacg ggcgctcgga
     1441 ccgggtcccg gtcgaggacc gcttctactt gggcggcgcc aactccgttc gcggctacga
     1501 cgaagcgagc ctgggaccgc gcagcgtcga atccggcgaa tcggtcgtcg acgggggaga
     1561 gttcatggcg cttgccaacg tcgagtttcg ctatccgctc ccgctcttgg gtcgctggaa
     1621 cttcgcgggg acgttcttcc tcgacggcgg caacgtgtgg ggcagcgcat cggacgtgac
     1681 ggcgagcgat tttcggctct cgagcgacgt gtccgagacc gaagtggacg acttccggta
     1741 cggaattggc ttgggtgtgc gttataacac gcccatcgga ccgattcgcg tggattgggg
     1801 ctatcccctc aagcccgatg tctacaccga cgagagtgga actttctatt tgagcctggg
     1861 tcaaatcttc tagcgcatgg cgaattccga ccagcagata cgtcccgagc gacggcgcag
     1921 caacgtccgg cgcatctcgg cggtgatcgc ggtggtgatc tcgatcgcgt tggcggtcgt
     1981 tgcttaccgc gaaagccgga ttctacccga acgcatcgcg gggtacgtca accgccacta
     2041 cctggccgga acgcccttcg agttctccct cgacggcgtg tcggggacct tcgtgcgccg
     2101 gatcaatctc cagaatccgg tcctgcgcta ccactccccg gaggcgtcct acaacgtctt
     2161 tcgggccgac cgcatctcgg tcacctacga gttgattccg gtattcgcat ttcgcctctt
     2221 ggtcgacgac gtcgagctcg agaacgtggt cgttcacttg cgccaggacc aggaagggcg
     2281 gttgatcatt cccggcaccg gcgtacccaa gccgggggcg aagaagaagg cggtctcccc
     2341 ggtggtcgac gtacgccact ttcgcatcga cgggctcgcg ctgaccttcg gcggaaacga
     2401 gacccagctc gcgatccgcg acgtcaatct ggacggcgcc ttcgcctacg atcgcggcgt
     2461 ggggcacctg tcgatcgaag agggccgcgc ctatctcctc gataccggga ccacggtgga
     2521 ggcggtgcgg ctcaacgcgc gctccgactg gcgcagcatc tttgtcgatg atttcgcggt
     2581 caaactggac tcgtccttcg tcgtggccaa gggcgagttc cgcgagggcc gtttccagga
     2641 cgtggagctg gtgctcaacc cgatctcgct cgaggagctc caccagctgg gtctgattcc
     2701 cgacgagcac ggcaccttcg acgcgcgcct ctcgctctcg ggaacggtcg actcgctcgg
     2761 cgtgcgcggg cgcgtctcgg gcgaggggct gggtgtggcg ctcgaaaacg tggacgtcga
     2821 ggggcacgtg acgccgcgcg cgctcgacct cgaccgtctc gcgggcaagg tgttcggctc
     2881 ctcggtcgac ggtgcgtttc ggatcgagat cgagtccgag gatttcgtgt tcgacgggcg
     2941 cgtcgcggac ctggacctcg ggcggggctt catcgaggac tccgacctgc cgcccatgtc
     3001 gctgaacggc gcggtccgcg tcgagcacac caaggcggtt gggcgctatg tgtggcgcgg
     3061 cgaactggag cgcggcgtga tcgacgggta cgagaccttc gacgtgagcg ccgcgggcgt
     3121 gtgggtggac ggcgtgggtc tgaccctcga ccgtttctcg acggtgcggc cgagctatcg
     3181 gatcgagggc tcggggacgg tcgcggacca aggcccggcc aacgtcgtgt tcaaggccga
     3241 gggcaccgac ttctcctact tctggaatta cttcgagctg ccgcccgtgt ccggggcgat
     3301 ggcgctgacc gggcacctcg agggcccgct cgacgacttc caggtcaacg tcaacggcac
     3361 cgtacgcgac ctcgcctggg agttcaccga agtcgacagc ggcaccgtgc aggcggaagt
     3421 ccgccgcgcg ggaaccctcg cgcctgaagt gacgctgtcg ctctccggac gccgcgcgcg
     3481 cgtctacgga cacccggtcg tgaacccatc cctgctcatg gacgtcgata cgacctcggt
     3541 ccgcatccac gacgtgcgcg tgacgcgcag cgactccacc ttcgtcgccg acttcgacgt
     3601 ggtggcgcgc gggaaggaat cgcgcgtctt cttgaagcac gcagctttgg agacgcccaa
     3661 cgagagctgg aacacgaccg gtccctctgt gattgacgtg agcgaggacg cggtcgtggt
     3721 cgactcgctc gtcctcgcct cctcgcgcgg cgagttcggg tactcgggga cgcgtcgcgt
     3781 acgcgaaaag cgcatcgacg gtcacgtctg gggccgcaac gtcaatctcg ctattttccg
     3841 cgacgtcctg cgcgtgccca tcgcgatcga gggcgtgggc accttcgacc tgcacttgga
     3901 gggaagcgaa gagaacccgc gcgcgcgcct gtcggccgcg atcacgcgag gactggtcga
     3961 cagcgtggcc ttcgacgccg cgcgcgccac cctctcgttc gacggcgagc gctaccggct
     4021 gagcgagctg tgggtacagg agggcgacga tacgctgacc gcgcaaggcg agtgggcgtg
     4081 cgacctcgcc ccgcgcgttc tcgcgcgcga ggaccgtccg gcgtcgctgt ggcgcgcccc
     4141 catttcggtg cgcgcgcgcg tgggccagta cgacctctcg tcgctgttcc gtgcgatgca
     4201 tcgaccgacc gacgtggcgt cgacgtacac cggcacggtg acggtggggg ggaccctcgg
     4261 ggaccccgag ctgcacacgc ggggcgagtt catgccgctg cccggtccgg ggcacccgat
     4321 tccgcccacc caggtcgacg ccgcctatcg caacggcgct ctgcgcatcg cgaggctcga
     4381 catatcgcag gtcgtcaacg cgcgcgcgac ggccaccatc cccatgacgg tctcgttccg
     4441 cgacggcgcg cgcatccaga ccgaccgccc gatggaggcg cgcatcgaga tcgacccgct
     4501 cgccaacgac gcgctgtcga acttcacacc ctacgtgcgc gacgtctcga acctgcgtgg
     4561 cgtcttgtcc gggcgggtgg acgcgagcgg aaccccgtcg tcgccgcgcc tgacgggcgc
     4621 gctttcgttt tcgcgcggcg agctgcgcgt ggccggcgtg caagagggcg cctccggcat
     4681 cgccatgcgc gtcgatttcg tcgacgacgt gattcggctg acgtcgctga ccgcgtcgtc
     4741 gggcaagaag gggtcgctgg tgggcgcggg ctgggcgcgg gtgtcgaact accgcctggt
     4801 cgattaccag gtcgacgtga ccgcacacga gttccccctg cgatcgattc ccgacgtcga
     4861 ggtggtcctg ggcggcacgc tcaccgcacg cctgtccgag tggagtggcg ggggccggat
     4921 tcccacgctc accgggcgct tgaacgtgaa ggaagccgtc atctcgaagg acctctcgag
     4981 cggtaccaac accgggtcgg agttgacgcg gcccaccgcg cggcccgact ggctggccag
     5041 catcgacgtg gttgcgccca agaacgtgtg ggtgcgcaac gaggacctga acgtagagct
     5101 gggcgcggag gacctcgtgt tcctgcgcga cgagcgcggc atgtactttc gcggcgagct
     5161 gacggttctg cgcgggtcgt acaaactcta cggcaacaag ttcaccatca cccaggggac
     5221 gatggatttc tcggcctccg agacgctgcg gccgtcgatg aacatcgagg cgtacacgcc
     5281 gcaccggggc ggcgacgagg tcgacaatcg catctatctg gtgctgtcgt ggccatacga
     5341 caagctcgag ccgcacatct cgctctcgta cgacgagccg ggctactcgg aatcggacat
     5401 ctggcgcatg ctcggtggca acatcgtctc ggggggtgtc gccaccaata cgctcgagcg
     5461 cgcgatcaac gcccagatgg ccgggggctt caccgtcgat gtggaccagc gcccgatcga
     5521 ggaggtgggt gccgccgacc agagccagga gaccctgatc ggggtcggga aatacctctg
     5581 ggaggacatc tacctccagt accggcgggg gctctcggtg ggcggggaac aggaattcaa
     5641 cgtggaatac cgactgagca acaagttctt gctgcgctcg cagttcattt acaattcgcg
     5701 taggaaccgt acgggaatcg tcgggcagaa tacggacgaa ttcaacctcg acctcaagta
     5761 taggttcgag tactaaaggc ggaagaattg cttgtccgta tgggcgcggg ctccgtataa
     5821 ttgatgcgct cgcccccttc agggaacggc gaaccccgcc cgccgaaccg tagcgtccag
     5881 ggcagtatca tgaagctaca ctccactttc gcggccatcg ccaccggggt cctcctcgga
     5941 gtgctatacc cggccacggc ttcggcccaa tacttcgggc gcaacaagat ccagtacaac
     6001 gactacacct ggcacgttct ctcgactccc catttcgaca tccattatta cgaaggcgcc
     6061 gaggcctttg ccgtgcgcgc ggccctggtg ctcgaagacg gctacgagga gtacacctac
     6121 aagctcaagg tcgtgctccc gtggaaagtg ccggtcatcc tgtattcgaa ccacgcggat
     6181 ttcctcgaga ccaacgtaac gtcgggccag ttgagcgagg gcgtgcaggc gttcgcggag
     6241 ccgtcgcggc ggcgcatcgt gctgccgttc acgagctcgt tcaaagaatt ccggcacact
     6301 gccatccacg agctcgcgca cgtgttcacg ttccacatcg tctacaatcg gatgttggac
     6361 aacgtgttca cgcgcaacta tctgttcgcg atgccgttgt gggtggccga gggattggcc
     6421 gagtacctgg ccgaagggtg ggacgccgac gcggacatgt tcatccgcga cgccgtcatc
     6481 caggactatc tgtacccgtt ccacttgatc agcgggttct atgtatataa ggagggccag
     6541 tcggttttca attacatcgc cgacacctac ggccacgaga aggtgctcga gatcctcaac
     6601 gcgctcgcgg ggacgcgcag cgccgaggcc gcgctccagc gcacgctcgg tctcaacgag
     6661 gatcagttgt acgagcagtg gtcgaaggcg ctgcgcaagc actactggcc gctgtatccc
     6721 gacaagcagg agctcgacga cgtcggccgg cgcttgaccg accacatcaa ggatcgcgcc
     6781 tactacaaca ccaagcccgt gctctcgccc gacggcgagc acatcgtgtt tttctccgac
     6841 cgctcgggga tgatcgaggt gcgcgtgatc tccgcgctgg acgggacgga agtcgcgggc
     6901 gtcgtcaccg actcgcgcac caacaagtac gagtcactcc acctgctgac cagctcggtg
     6961 tccttcgacc cgaccggcac cttcgtcgcc ttcgtcgcca agtccaaggg acacgacgcg
     7021 ctcttcgtcc gcaatttcag gaccgcggaa gagcgcacct acgagatcga gtccaacggg
     7081 ttgacctcgc cggcgtggca cccgacgcgc aacgagatcg tggtgtcggc caccttccac
     7141 ggccaaaccg acctgctgac cgtcaacttg gacgacggca ccaccaaacg actgaccaac
     7201 gacccggccg accagctcac gccgcgcttc tatcccgacg gcagcaagat cgtgttcgtc
     7261 cactttcccg agatcacgat cccggtgccc cccgacttcg acggcgacaa tcggaccgcg
     7321 ttgagcgaaa tggacttcct cgacccgaac aacgtgaaag acgacgccaa ctacgacatt
     7381 tacgaattcg acgtcgcgac gggcaaacag cggccgctcg tgcattcgcc gggcgacgac
     7441 accgagccgg ttattctttc cgatgggaag acgctgatct acgcctccga cgagtcgggg
     7501 gtcaacaacc tgcacgcggg caacgtggac acgggtgtca cctaccgctt caccgacgtg
     7561 ctgggcgggc tattcacccc gacggtgcac gaagagaagg gacgcatcgc gttctcggcc
     7621 ttcgtcaagg ggggctggga catcttcgtg tccgatgacc tgccgaccat gctgggacgc
     7681 aagtacgcgg agccggcacg ccgcgtgttg gcgcacgacc ccaaggtggc cgcgaaggac
     7741 ctcgagatcg cgcagaaggc ggcattcgaa gccgagacgc cggcggcagg ggacagcgcg
     7801 caagcggccg cgctcgagaa gagtctggcc gcgcaggctt cgaccgcgcc gacggtcaac
     7861 gggatggtgg agggcgttgc cgattcgacg gcgcaggcgc ttgcggacac gaccgccgcg
     7921 gcggccgatt cgaccgcgtc ggaagcgttc gagatcgact tgaaggacta ccaaccggcg
     7981 attcgaccgc tcgacccgcc cgcaccggcc gagtcgaccc agtcgtcttc gatcggcgtg
     8041 cgcccgcgcc tggagggtgt caacgccccc gtcggcgccg acgaatcggt gttccgcggc
     8101 gccaccgtca atcgctacca caccaagctg gcgcccgatt tcatcgggag cggcggtggg
     8161 ctgtacttct cgtccggttt cggtttcgga ttgtcgaaca gtgtcgcgct ctcggacatg
     8221 ctgggcgacc accacctgat gttcgcgttc agtttgtacc aggacatcgc caactccgac
     8281 gtcctggtcg cctacacgta cttgaagcgg cgcgtcgact tctccttcgg cgcgtatcag
     8341 ttcagcgggt actacgacag ccgggtgagc tcggtgggcg agaccttctc gaccaaccag
     8401 ctcttcagcg agcgcaacta cggtgtgttc ggcctgatga gcgtgccctt caacaagttc
     8461 taccgcatgg aagtcgacct gcaggcgtat tattcggacc gcgagttctt ccaagacatc
     8521 gaccccacgg tcgactacca gtacaccacc gtggagaaat cgaaggtaag gctgatcgag
     8581 ccggcgctcg cgttcgtcca cgactcgtcg ttctacggcc ccttcggccc ggtggacggc
     8641 tcgcgctggc gcatctcgct ggcgcgcgga gtggcgttcg aggagcagga cgtctcgcgc
     8701 accaccggct acatcgactg gcgctggtac cgcacgctct tctggcgcaa ttccttggcg
     8761 ctgcgcttca gcggagcggc cagtgtgggt gaggacccgc gcgtgttctt cctgggcggg
     8821 cccacgcggc tgcgcggcta cgaatgggac cagatcgaag ggtcgcgcat gtggtacggg
     8881 agcttcgagt atcgcttccc gctgatcgat gcgctcatct tggggtggcc ggggcgctgg
     8941 gggttcacca acatcggtgc caccgccttc ttcgacgtcg gtgccgcgtg gaaccggggc
     9001 atccccgacc tgttcgtcga cgaccgcctc gaccaggcct attccgacat cggcttcggg
     9061 ctctacatgt acgccggcta tttcctcttg agcttccagt tcgcctggcc gaccgacgtg
     9121 agcacgatca acgacgacat gaagttccac ttctatatgg gcccgacctt ctagccctcc
     9181 cgcgcatccg catgcatcgg ctcgctgcct tcgccttctt gttggcggcc gcctcgtccg
     9241 ctggtgccgc caatccatat ccggtccccg aaggcgcgcg atctgcggcg atggaatacc
     9301 gatctcagtg ggacgttgta gaacactggg ttgcgctttg cgtcttcgat tgagggcatt
     9361 ggtggctttt ctcgagcgcc gaccttcagt gtccttacct ttgtcggtga ttcgctaggt
     9421 cataatttta tccgtcttgc gaccgatgac gggatttatt tcgtgtccat gaatcacagt
     9481 gcggtaggtc tgttcttcga aatggggggc gtggtcttgg tgccggagga tcgggccgtg
     9541 ccgcttattc gcgcgcaaat acttaacccg gcaagggaat agcttctagc agttccacac
     9601 gaccattccc cgacgttcgg ggatccttct ccgccggtga gcccgccgcg cgcgacaaat
     9661 tcgtactccc ggctcctagc ttcgtgtgct aacttcggat ggttgcacac gccatgtcaa
     9721 acgtaaccaa cctgaacgac cggcagcgcg aagccgttct caccaccgaa ggccccgtcc
     9781 tcgtcatcgc gggggcgggc agcggcaaga cccgcgtgct gaccgaacgc atcgcccacc
     9841 tggtgggcga cctgcgggtg tctccgtacg agatcctggc cttcaccttc accaacaagg
     9901 ccgcgcgcga gatgaaggac cgcctggagc gcgcgcaccc gggatcgacc gagatgctgt
     9961 gggtggggac gttccactcg accggtgtcc gcatcctgcg ccgccacggg gattccgccg
    10021 gcgtgaagcg ggacttttcg atctacgaca ccgacgacag caccgggctg gtcaaggcga
    10081 tcctcgcgcg caaccccaac gacgcgcgct tcgtgaagtc gccgcgggtg ctgcgcgacc
    10141 gcatctcgcg catgaaaaac gacctcatca cgcccacgga cgccgccgaa cgcgcggtct
    10201 cggcgggcga gcagcgcgtc gccgcgctct acgccgagta cgagcgcgac ctgcgcaagg
    10261 ccaacgcgct cgacttcgac gatttgatca acaaggtggt agagctgttc gcgaccgacg
    10321 accgcgtgcg cgagctgtac gcgcgccgct tccgctacgt cctggtcgac gagttccagg
    10381 acaccaatcc catccagatg gcgatgatcg acgggctggc gtccgtgcac ggcaacttgt
    10441 tcgtggtggg cgacgacgac cagtcgatct acagctggcg cggcgcgcgc gtggagcaca
    10501 ttctcgagtt cgagggtttg tacccggcaa ccaaagtcat acgattggag cagaactacc
    10561 gctcgaccaa gaccatcctg gacgccgcca acggggtgat cgcgcacaac c
//
LOCUS       DASPGK010000225         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1581308, whole genome shotgun sequence.
ACCESSION   DASPGK010000225 DASPGK010000000
VERSION     DASPGK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581308"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VEC56_06150"
     CDS             complement(<1..421)
                     /locus_tag="VEC56_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13770.1"
                     /translation="MVTRLTFLISTILIASPAAAQWQPNGVPLNGGGVPQVVTDGSDG
                     AIVAWEYWDPWTGQWDYGMGQRLSPGGSELWQPYGHVLCGSSDQNVPQLVSDGAGGAI
                     FVWLDNRNGYLNIYAQRIDASGAEQWEPCGVAVRIGNS"
     gene            585..956
                     /locus_tag="VEC56_06155"
     CDS             585..956
                     /locus_tag="VEC56_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV13771.1"
                     /translation="MRVSYAIVFVGDMKRSVAFYRDVIGLPVKFETSVWTEFATEGAT
                     LALHASEAPGASNENPLAVAAGRCRPGLSVSNLDAFHQRMLEKKVVCVQEPKDLFGAR
                     IAQYLDPDGLAISVSEEHRGK"
     gene            complement(1012..>2248)
                     /locus_tag="VEC56_06160"
     CDS             complement(1012..>2248)
                     /locus_tag="VEC56_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV13772.1"
                     /translation="GPAFNLTRAVDLPAERMNEYVGWYAGEVEDAVVVTPGSEGGLRF
                     FHLGRLSGTLRPLARDEFAMASLDQASTVLRVRARFTRDASGRVAGLDIGDDPVAHRT
                     RLARIATPPFRQQHVDFRNGDVRLGGTLFSPADDLAHPAIVFIHGTGYQTADRGYEMS
                     LTTTFLQRGIAVLLFDKRGCGSSSGSWRTASLSDLAEDVAAAVAALGSNPAVDADRIG
                     VYGVSEGGWVAPMVAHSAAIAFVINQGGPAVTPLEDELDDLTTGIARLGLAREDSISA
                     MNLARAWVELYRSAEAMPRYRAALDAARARPWFAPFESRFPTSEDDWEVQWWRKRGRL
                     DPLPLWEAVPVPALVLIGEKDATMDVAKNVALFERVEQSGRVELRVIPDADHGLFVSG
                     GLAANFPDAVADWILATLE"
BASE COUNT          410 a          766 c          708 g          364 t
ORIGIN      
        1 cgctgttccc tatgcgcacg gcaacgccac agggttccca ctgttcagca cccgatgcat
       61 cgattcgctg cgcgtagata ttcaagtacc catttcgatt gtcgagccag acgaatatgg
      121 caccgcccgc tccgtcggag accaattggg gcacgttttg gtcggacgag ccgcagagga
      181 catggccgta cggttgccac agctcgctcc cccccggcga gagacgctgt cccattccgt
      241 agtcccattg tcccgtccag gggtcccagt actcccacgc cacaatggcg ccgtcggaac
      301 cgtccgtgac aacttgggga accccaccgc cattgagcgg gacgccgttc ggttgccact
      361 gcgcggcggc gggggacgca attagaatag tcgaaatgag aaaagtgagg cgggtgacca
      421 tagggcctct cttccgccgc ggtagacgac gcggcgttgg cttggggcgg tacacaagtc
      481 tacaccgcgg caccgcaagt gtaaagaccg cggtagagca accactaccg tttacgaccg
      541 gcgtccgatc gtgtgctact cttgacgggc gaaaggagcc ccctatgcgc gtgagctacg
      601 ccatcgtttt cgtgggagat atgaagcggt ccgtcgcgtt ctatcgcgac gtgatcggac
      661 ttcccgtcaa gttcgaaacc tccgtctgga ccgaattcgc caccgaagga gcgaccctcg
      721 ccctccacgc gagcgaggcg cccggtgcct cgaacgaaaa tcccctggcc gtcgccgcgg
      781 gccgctgccg gcccggcttg agcgtgtcga acctcgacgc cttccaccag cgcatgctcg
      841 agaagaaggt ggtctgcgtc caagagccca aggacttgtt cggcgcgcgc atcgcgcagt
      901 acctcgaccc cgacggcctc gcgatctccg tcagcgaaga gcaccgggga aagtagtggg
      961 actggacgtg ttgtggaacg acgcaccgcc ggcaatagcg ccgacccggc gctactccaa
     1021 cgtcgccagt atccaatccg cgaccgcgtc cgggaagttc gccgccaagc cacccgacac
     1081 gaagagtccg tgatcggcat ctggaatcac gcgcagctcc acgcgcccgc tctgctcgac
     1141 gcgctcgaac aacgcgacgt tcttcgcaac gtccatagtg gcgtcttttt cgccgattag
     1201 gaccaacgcg ggaacgggca cggcttccca caagggaagg gggtcgagcc gcccgcgttt
     1261 gcgccaccat tgcacctccc aatcgtcttc cgaagtggga aagcgcgact cgaagggagc
     1321 gaaccatggc cgtgctcgcg cggcgtcgag tgcggcgcgg tatcgcggca tcgcttcggc
     1381 cgatcgatag agttcgaccc acgcgcgcgc caggttcatc gccgagatcg agtcctcgcg
     1441 cgcgagcccg agtcttgcga tgccggtggt gaggtcgtcc agttcgtctt ccaacggggt
     1501 aaccgcggga cctccctgat tgatcacgaa cgcgatcgcg gccgagtgcg cgaccatcgg
     1561 ggccacccaa ccgccttcgc tgaccccgta cacgccgatg cggtccgcgt cgacggccgg
     1621 attcgacccc aacgccgcga ccgccgccgc gacgtcttcc gccaaatcgg agagcgatgc
     1681 cgtgcgccac gagccgctcg atgacccaca gccacgcttg tcaaagagca gcaccgcgat
     1741 cccgcgctgc aagaacgtgg tcgtcaggga catctcgtag cctcgatccg cggtctgata
     1801 gcccgtcccg tggatgaaaa cgatcgcggg atgggcgaga tcgtcggcgg gcgaaaacag
     1861 ggtgccgccg agcctcacgt cgccgtttcg gaagtccacg tgctgctggc gaaagggcgg
     1921 cgtcgcgatg cgcgcgagcc gggtccgatg ggcgacgggg tcatcgccga tgtcgaggcc
     1981 cgccacgcga ccggatgcgt cgcgcgtgaa tcgtgcgcgg acgcgaagca ccgtggatgc
     2041 ctggtccaac gaggccatgg cgaactcgtc gcgcgccagc ggccgcagcg ttcccgagag
     2101 ccggcccagg tggaagaatc gcagcccgcc ttcgcttccc ggcgtgacga cgaccgcatc
     2161 ctcgacctcg cccgcgtacc agccgacgta ttcgttcatg cgttccgcgg gcaggtcgac
     2221 cgctcgcgtc aaattgaacg cgggtcct
//
LOCUS       DASPGK010000226         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_995085, whole genome shotgun sequence.
ACCESSION   DASPGK010000226 DASPGK010000000
VERSION     DASPGK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995085"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VEC56_06165"
     CDS             <1..359
                     /locus_tag="VEC56_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13773.1"
                     /translation="LAAGNAEGARRRLEWCLLQAPRYSQARIALADALMDLDRYQDAR
                     RVIAALNAYAPDNTRGLYYAAYINAQLGERDEAKRYLALLFTIERDPDLIQRARALES
                     YLEQGVMPRPGAPRDG"
     gene            complement(403..477)
                     /locus_tag="VEC56_06170"
     tRNA            complement(403..477)
                     /locus_tag="VEC56_06170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Val,seq:cac)
     gene            complement(468..1457)
                     /locus_tag="VEC56_06175"
     CDS             complement(468..1457)
                     /locus_tag="VEC56_06175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV13774.1"
                     /translation="MQLHARMVLTVAAATVLAISCSKKKENAAHAFPDSLVDDEVVAT
                     VNDEPITARELKVLAYIGSGQIDSTRAASFNLRLLDQMIDRKVFSQEAKAAGVAVPDS
                     VVANVLDQFVRQFGGETQVDQMLAPMGLSREQFRDAIQRDLTIRKYIDERIMPSIAVS
                     DADTRAFYDQNPEMFAGTDSVHARHIILLSHDEDTDQAKSDRRAQMAAIRQRAIGGED
                     FAKLAMQFSQDNAAQNGGDLGYFPRGMMVKPFEDAAFALKKGQISDVVETQFGLHLIK
                     CIDKKPARAVPYDDAKGSIDAMLKQRSLSAELQNRLQKSREAAIIVRSYETGA"
     gene            complement(1523..2143)
                     /gene="nth"
                     /locus_tag="VEC56_06180"
     CDS             complement(1523..2143)
                     /gene="nth"
                     /locus_tag="VEC56_06180"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXV13775.1"
                     /translation="MQRALDGGKLPSVSEIARRDRDPFRILISTLLSLRTKDDVTDAA
                     SARLFALAPTASKLARLDPKRIEKTIYPVGFYRTKARTVRDVARRLETEFGGKVPSTM
                     EALLGFKGVGRKTAALVVSLGYGVPAICVDTHVHRISNRLGWVRTRTPDQTEHALMEL
                     VPRTHWIEVNEIMVGFGQRVCTPLSPRCSRCPVARSCARVGVAKHR"
     gene            2269..2601
                     /locus_tag="VEC56_06185"
     CDS             2269..2601
                     /locus_tag="VEC56_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HXV13776.1"
                     /translation="MPAKKQPVAKTRTRTKADPKAFAKTRARLDQKRAKRRQKMLWVH
                     YWLLCPKCGGDMFVQSTLGIRFEVCRACHGIAIDGEEMTLLLAHLDPGKALRAILKKS
                     KKPDETEI"
     gene            complement(2616..4058)
                     /locus_tag="VEC56_06190"
     CDS             complement(2616..4058)
                     /locus_tag="VEC56_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13777.1"
                     /translation="MFDRMWMGSGKPTNGGFERPQSIAIGTAEQVVVVDRQLSSTWPL
                     RIQIFGTETSVVTAELEAFTDDGVNPLAPNATLPASGCPEGDGDHLVVTVAIDPSEVA
                     GPIPASALVLVLPSGGAAVSYAPVNADEDATLVNGMYRTTFTMRKFGGCQSSDVVVAL
                     NGVGIGSVPVVLRSPDWEAGGSDGAVNLGDFAWFGGHYTSPPKPYSECADHRAAYGAV
                     NLGDFGHFGAHYAHTSGGGSLLSGTLPAADGTLRVELEESNPVVGERRLWARLVLEGV
                     EPFKAMVVALRNEHPDLEYLGWTPSAAYANGTACIELTRGGVKQVFLGVMGTGGVSGG
                     SVELGRVEFAIQGDEALTLTDEDLSFLNAELLSSADKVLAFSGLRLERTINAPSYEFE
                     LAQNYPNPFNPSTTIAYSLAQASDVELTIFDVRGSHVKTVVRERRDAGVHRAPWDGTD
                     NRGNRVASGVYFYRLTAGSFRATKKMVLLR"
BASE COUNT          772 a         1465 c         1350 g          756 t
ORIGIN      
        1 ccctcgcggc cggcaatgcg gagggcgcgc gacggcggct ggagtggtgt cttttgcagg
       61 cgccgcgcta cagccaggcg cgcatcgcgc tggccgacgc gctcatggac ctcgatcgct
      121 accaagacgc gcggcgcgtg atcgcggccc tgaacgcgta cgcgcccgac aacacgcgcg
      181 ggctctacta cgccgcctac atcaacgcgc agctgggaga gcgcgacgaa gccaagcgct
      241 acctcgcgct cctcttcacc atcgagcgcg accccgatct gatccagcgt gcgcgcgcgc
      301 tggagagcta cctcgagcaa ggcgtgatgc cgcggccggg agcaccacgc gacggctaga
      361 aaacgcgacg gccgcgacgc aacgcgccgc ggccgccgaa agtggtgggg gctactggat
      421 ttgaaccagt gactcctacc gtgtgaagat agtactctcc cgctgagtta agcccccgtt
      481 tcgtaactgc gcacgattat cgccgcctcg cgactctttt gcaagcggtt ctgcagttcc
      541 gccgacagcg aacgctgctt gagcatcgcg tcgatgctgc ccttcgcgtc gtcgtacggc
      601 accgcgcgcg ccggcttctt gtcgatgcac ttgatgagat gcaggccgaa ctgagtctcc
      661 acgacgtcgc tgatctggcc cttcttgagc gcgaaggcgg cgtcttcgaa cggcttgacc
      721 atcatgccgc gcgggaagta gcccaggtcg ccgccgtttt gcgccgcgtt gtcctgcgag
      781 aactgcatgg ccagcttggc gaagtcctct ccgccgatcg cgcgctggcg gatcgccgcc
      841 atttgcgccc gccggtcgct cttcgcctgg tcggtgtctt catcgtgcga caacaagatg
      901 atgtgccgcg cgtgcacgct gtcggtgccg gcgaacatct ccgggttctg gtcgtagaac
      961 gcgcgcgtgt cggcatcgct gaccgcgatc gacggcatga tgcgctcgtc gatgtacttg
     1021 cgaatcgtga ggtcgcgctg gatcgcgtcg cggaactgtt cgcgcgagag ccccatgggc
     1081 gccagcatct ggtcgacctg ggtctcgccg ccgaactgcc gtacgaactg gtcgagcacg
     1141 ttggccacga ccgagtcggg cacggcgacg ccggccgcct tggcttcctg cgagaaaacc
     1201 ttgcggtcga tcatttggtc gagcagacgc aaattgaacg acgcggcccg ggtcgaatcg
     1261 atctgtcccg aaccgatgta cgcgagcacc ttgagctcac gggcggtgat gggctcgtcg
     1321 ttgaccgtgg cgacgacctc gtcgtccacc agcgaatcag ggaacgcgtg cgccgcgttc
     1381 tccttctttt tcgagcatga gatggcgagc acggtcgcgg ccgcgacggt cagcaccatc
     1441 cgagcgtgta actgcataga taggtgtcct ttgagttagg ccggggatga tagcagaccc
     1501 ggcggcgggg cgcaatcggg gttcagcggt gtttggccac cccgacccgc gcgcacgacc
     1561 gtgccaccgg gcaccgcgaa cagcgcggcg agagcggcgt gcacacccgc tggccgaagc
     1621 cgaccatgat ctcgttgacc tcgatccagt gcgtgcgcgg gacgagctcc atgagggcgt
     1681 gctcggtctg gtcgggcgtg cgcgtgcgaa cccaccccag ccggttcgag atccggtgca
     1741 cgtgggtgtc gacgcagatg gcgggaacgc cgtagcccag cgacaccacc agcgccgccg
     1801 tttttcgtcc cacgcctttg aagccgagga gcgcttccat ggtactcggg accttgccgc
     1861 cgaactccgt ctcgaggcga cgcgccacgt cgcggacggt gcgcgccttg gtgcgataga
     1921 agccgaccgg gtagatcgtc ttctcgattc tcttcgggtc gaggcgcgcc agtttgctcg
     1981 cggtgggcgc gagcgcgaac aagcgcgcgg acgcggcgtc ggtcacgtcg tctttggtgc
     2041 gcaaactcaa gagcgtcgat atcaggatgc gaaacggatc gcggtcgcgg cgcgcgatct
     2101 ccgagaccga cgggagcttg ccgccgtcaa gcgcgcgctg catcgcggcg aggacgaccg
     2161 cgacgcgtgg gcgtacggcc ggcgtgcgct tcgcacgcga tggtttggat ggagccatag
     2221 gattgacagt cgatgaagtc agtgggagaa tgccggcaag atactcctat gcccgccaag
     2281 aagcaacccg tcgccaagac tcgcacgcgc acgaaagccg accccaaggc cttcgcgaaa
     2341 acgcgcgcgc gcctcgacca gaagcgcgcg aagcggcgcc agaagatgct atgggtccac
     2401 tactggcttc tgtgcccgaa gtgcggcggg gacatgttcg tgcaatcgac cctcggcatc
     2461 cgcttcgaag tctgccgcgc ctgccacggc atcgccatcg acggcgagga aatgacgctc
     2521 ctgctcgccc acctcgaccc cggcaaggcg ctgcgcgcga tcctcaagaa gtcgaagaag
     2581 ccggatgaga ccgagatata gggtcggagt cgagattacc gcaagagcac cattttcttc
     2641 gtggcccgga acgaccccgc cgtcaggcga taaaaataga cgccgctcgc gactcggttg
     2701 ccccgattgt cggtcccgtc ccaaggtgcc cggtgaacgc ccgcgtcccg ccgctcccgc
     2761 acgaccgtct tcacgtggct cccgcgcacg tcgaagatcg tgagctcgac atccgatgcc
     2821 tgcgccagcg agtacgctat cgtggtcgac gggttgaacg gattcgggta gttctgggcc
     2881 agctcgaact cgtagctcgg cgcgttgata gtgcgctcga ggcgcagccc ggagaacgcg
     2941 agcactttgt cagccgacga cagcaattcc gcgttcagga acgacaggtc ctcgtcggtc
     3001 aatgtaagag cttcgtcgcc ctgaatggcg aactccaccc tcccaagctc gaccgaccca
     3061 ccgctcacac ctccggtgcc catcacaccc aagaatactt gcttcacgcc gcctctcgtc
     3121 agttcgatgc acgccgtccc attcgcatac gccgcgctcg gcgtccaacc caagtactcg
     3181 aggtcggggt gctcgttgcg aagcgctacg accatcgctt tgaacggttc gaccccctcc
     3241 aagaccaagc gggcccaaag ccgtcgctcg ccgacgaccg ggttcgattc ctccagttcc
     3301 actcgaagcg tgccgtccgc ggccggaagc gtgcccgata gcagcgaacc tccgcccgac
     3361 gtatgcgcat agtgtgctcc gaaatgacca aagtcgccta ggttgacagc cccgtacgct
     3421 gcgcgatggt ccgcgcactc cgagtacggc ttcggcggcg acgtgtagtg gcctccgaac
     3481 catgcgaagt ccccaagatt caccgcgccg tcgctccctc cggcttccca gtcgggactg
     3541 cgcagcacca ccggcacgct tccgataccc acaccgttta gcgccacgac tacgtcgctc
     3601 gactggcacc caccgaactt ccgcatcgtg aaagtcgtgc gatacatccc attcaccagg
     3661 gttgcatcct cgtcggcgtt caccggagcg tacgaaacag cggcaccgcc cgatggcagc
     3721 accaggacga gcgccgaggc cggaatcgga ccagccacct cgctcggatc gatcgccaca
     3781 gtgaccacga gatggtcgcc gtctccctcg gggcagccgg atgcgggcag cgtggcgttc
     3841 ggcgccaggg gattcacgcc gtcgtccgtg aacgcttcca gctcggctgt gacgaccgac
     3901 gtctcggtgc cgaatatctg gattcggagc ggccacgtcg aactcagctg gcgatcgaca
     3961 acaacgacct gctcggcggt accgatcgcg atcgattgag gcctctcgaa accaccgttt
     4021 gtcggcttgc cgcttcccat ccacatacga tcaaacatcc catcgctcgt aaagcgctgg
     4081 acgcgattgt tgtcggcgtc gctaacgtac acatttccgc ttggcccaac agccagacct
     4141 tggatttgat caaaggaccc cgcgtcgact cccgcctggc cggtcaccca gtcaagtaca
     4201 taactgccac cttgcaggat gccgcgcgcg atgcgatcgc tcgaggctac gtagatctct
     4261 tcgtttggac ctacagcgat ctgccagatc gggaaggtcg tgcccgtcgc ctgtgaggca
     4321 ttccatgtcg tgatcgcagt acc
//
LOCUS       DASPGK010000227         3983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_527508, whole genome shotgun sequence.
ACCESSION   DASPGK010000227 DASPGK010000000
VERSION     DASPGK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527508"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEC56_06195"
     CDS             <1..319
                     /locus_tag="VEC56_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV13778.1"
                     /translation="ITGPVTNDELALRSSIGLFLFVPPGVNERFIEQAGFRLVRQEDV
                     TANAALTSGRWRDARAKHREALLEIEGEERFEGLQKFFGAVHHLTSERRLSRMAYVVE
                     KRS"
     gene            467..2509
                     /locus_tag="VEC56_06200"
     CDS             467..2509
                     /locus_tag="VEC56_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV13779.1"
                     /translation="MDPRIVARVLSEIAETASETLELQDVFHRVAASVRLVIPFDHMG
                     VMRVVEGNRAVLHAATFMDDTEKKCVDHPCNLDDFSPRVRPHPGPTPRIDDAQKELDP
                     SYPMDAMILEGGTRSSLWHPFFSGDVFTGGVWIDSFQANAFNDEHQEVLGPIAALLGS
                     AVEHWRIWDAERRRRERLDQLELALGSLAESLDVREVFERLSNSVKAILPHDLVYLGG
                     LDESTRSIYTIAVAGEADIDPPTKPFQLTEQEAQRRELDFDILRDIPAQITPTTERNK
                     ILLATGMRSWLKVPVRLAGVVQASIGFFHRLPAMYSPEDVEVARRMADRVALVLSHQR
                     LAEEARVASEALERAQRLQVTIEALTRELESRGRTKVVGVSLPWKEVLRHVGRVASSD
                     TTVLITGESGTGKEVVSRLIHQGSPRAEKPFIAINCAALPEQLLESELFGHERGAFTG
                     AIATKTGRIEQAAGGTLFLDEIAEMSPMVQAKFLRVLQEREFQRVGGTRTLKADVRVV
                     AATNRNLAAAIARGDFREDLFYRLNVFEIRIPALRERPEDILPLAESFLEELGAAMGR
                     AAAGISREGREWMLEYPWPGNVRELRNAIERAILLCDGGLITRAHLPVTKPAPARAAV
                     VLEPATPLIPGDLEAMDRSYIEKALNQARGNKSKAARLLGLSRAQLYTRLEKYNIR"
     gene            2839..3087
                     /locus_tag="VEC56_06205"
     CDS             2839..3087
                     /locus_tag="VEC56_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13780.1"
                     /translation="MRTLKLATFGVVVALIAIIVLFSSSAFGGTGANVPPTESLSLRA
                     HSREVVAVPSDDAVGLSDGTEADPIWMPRGTFGLSYRF"
     gene            3163..3399
                     /locus_tag="VEC56_06210"
     CDS             3163..3399
                     /locus_tag="VEC56_06210"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HXV13781.1"
                     /translation="MSQQNVERVLGQLVTDEGLRHRFVMDPHATIYFVMEGGCDLNEC
                     EREGLLSLDLRMLVRFANSIHPCLQKTDLRGGVK"
     gene            3396..>3983
                     /locus_tag="VEC56_06215"
     CDS             3396..>3983
                     /locus_tag="VEC56_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV13782.1"
                     /translation="MRIRVLAAVLSVLAAVPVFGAERPLTLEEAVAFALEKNEGIFIS
                     RESAAAASANASGARGVYNPLLVLDGYWRETKQPVNSAFSGAPLGEGAPTTESLEGSA
                     VLSQYLPTGGTLSARTITAKGETNGTYDLLSPAYQTRVGVEFRQPLLRDFGMDAERVS
                     IKVADAERKRGSAELRIEVSNTVAEVERAYWNLAAA"
BASE COUNT          729 a         1289 c         1308 g          657 t
ORIGIN      
        1 catcaccggt cccgtcacca acgacgagct cgcgctgcga agctcgatcg gtctcttcct
       61 cttcgtgccg cccggggtca acgaacgctt catcgagcag gcaggatttc gactcgttcg
      121 acaagaggac gtgaccgcga acgccgcgct cacctcgggc cgctggcgcg acgcgagagc
      181 gaaacatcgc gaggcactgc tcgagatcga gggggaggaa cggttcgagg gactacagaa
      241 gttcttcggg gcggtgcatc acctgacgag cgagcggagg ctgtcgcgaa tggcgtacgt
      301 ggtggagaag cggtcgtaat cggactgact tcatcggccc ttcacgatcg accattcgcc
      361 gagtcgccga ttgaccccac ccacaatcaa acctctggcg ccgtctcgaa tcgcgcctta
      421 tcatagttga agcttcatcc agtaacccga agtccgaaag cctcccatgg acccaaggat
      481 agtcgccaga gttctgtccg agatcgccga gaccgcgtcg gaaacgcttg agttgcaaga
      541 cgttttccat cgggtcgcgg cctcggtccg tctggtcatc ccgttcgacc acatgggggt
      601 catgcgggtc gtggaaggga accgcgccgt tctgcacgcc gccaccttca tggacgacac
      661 cgagaagaag tgcgtggatc acccctgcaa cctggacgat ttctcgccac gggtccggcc
      721 gcatccgggc cccacgccac gcatcgacga cgcccagaag gagctggacc cgagctaccc
      781 gatggacgcg atgatcctgg aaggcggcac gcgctcgtcc ctgtggcacc cgttcttctc
      841 gggcgacgtc ttcacgggcg gggtctggat cgattcgttc caagccaacg ccttcaacga
      901 cgaacaccaa gaagtcctgg gcccgatcgc cgcgctgttg ggatccgcgg tcgagcattg
      961 gcgcatctgg gacgcggagc ggcgccgccg cgagcggctg gatcaactcg agctcgcgct
     1021 cggttcgctg gcggagtcgc tggacgtgcg cgaggtcttc gaacgattgt cgaactcggt
     1081 caaggcgatc ctcccgcacg acttggtcta tctgggcggg ctcgacgaga gcacgcggtc
     1141 gatatacacc atcgcggtgg cgggcgaagc ggacatcgat ccgccgacga aaccgttcca
     1201 gctcaccgag caagaagcac agcggcgcga gctggacttc gacattctgc gcgacattcc
     1261 ggcccagatc acgcccacga ccgagcgcaa caagatcctg ctggccacgg gcatgcgctc
     1321 gtggctcaaa gtaccggtgc gcttggcggg tgtggtgcag gcgagcatcg ggttcttcca
     1381 ccgcttgccg gcgatgtaca gtcccgaaga cgtcgaggtg gcgcgccgca tggcggatcg
     1441 ggtcgccctc gtgctctcgc accagcgcct ggccgaggaa gcgcgcgtcg cgtcggaagc
     1501 gttggagcgc gcgcaacggc tgcaagtgac gatcgaagcg ctcacccgcg agttggaatc
     1561 acgcgggcgc accaaggtgg tgggcgtatc gctcccgtgg aaagaggtgc tgcgccacgt
     1621 ggggcgcgtc gcgtcgtcgg acacgacggt attgatcacg ggcgaatcgg ggaccggcaa
     1681 ggaagtggtc tcgcgtttga tacaccaggg ctcgccgcgc gcggagaagc cgttcatcgc
     1741 catcaactgc gcggcactgc ccgagcagct gctcgagtcc gagttgttcg gccacgaaag
     1801 gggtgccttc acgggcgcga tcgcaaccaa gaccgggcgc atcgagcagg ccgcgggcgg
     1861 gacgctgttc ctggacgaga tcgcggagat gagcccgatg gtgcaggcga aattcctgcg
     1921 cgtgcttcaa gagcgcgagt tccagcgcgt gggcggaacg cgcacgctca aggccgacgt
     1981 gcgcgtggtc gccgcgacca atcgcaatct cgccgccgcc atcgcgcgcg gcgacttccg
     2041 cgaggacctg ttctaccggc tcaacgtgtt cgagatccgc atccccgcgc tgcgcgagcg
     2101 ccccgaagac atcctcccgc tggccgaatc gttcttggaa gaactgggcg ccgcgatggg
     2161 ccgcgcggcc gcgggtatct cgcgcgaagg gcgcgagtgg atgctcgagt atccgtggcc
     2221 cggcaacgtc cgcgagcttc gcaacgccat cgagcgcgcc attctgttgt gcgacggcgg
     2281 actcattacg cgcgcacacc tgcccgtgac caaaccggcg ccggcgcgcg cggccgtggt
     2341 gctcgagccc gcgaccccgc tcatcccggg cgatctcgaa gccatggacc gcagctacat
     2401 cgagaaggcg ctcaaccagg cccgcggcaa caagtcgaag gcggcgcggc tcctgggatt
     2461 gagccgcgcg cagctctaca cgcgcctcga gaagtacaac atccgctagc tcctctccgc
     2521 tcattccaga ttcgttcgtc cgctgctgtc gacgcgtcga caggttgtcg atccatcgac
     2581 aactccgttc tgccgtcacc cgctcgccgg tccttccgag cgtccacaac tccaaggaac
     2641 gtcacacctt agccgatacc ggaagaaatt tccggttctg gcacgaggct tgagataggg
     2701 gatcgccgaa gaaacgaacg cgcagcgcaa gatttcgatg cgcacgcggt cgcaaatcga
     2761 actcatcgct cgtcgcacag acgagacatc gaaacgaatc acccgtcgca ccgacgggac
     2821 atcgaaggag gaacctaaat gaggacactg aagctggcga cattcggcgt ggtggtggca
     2881 ctgatcgcca tcatcgtcct gttttcgtcg agcgcgttcg gggggaccgg ggccaatgtg
     2941 cccccgacgg agtcgctgtc gctgcgcgcg cactcccgcg aggtcgtggc cgtcccctcg
     3001 gacgacgcgg tgggactttc ggacggaacc gaagcggacc cgatctggat gccgcgcggc
     3061 accttcgggc tctcgtatcg gttctagagg cgaggcgccg ggcgttaccc agatagcagc
     3121 gcccggcgcc gatcgcctaa acgaatggca aggaacgttg aaatgagcca acagaacgtg
     3181 gaacgggtgc tgggccagct ggtgaccgac gagggtcttc gccaccgctt cgtcatggat
     3241 ccgcacgcga cgatctattt cgtcatggaa ggcggatgcg acctgaacga gtgcgagcgc
     3301 gaggggctac tgtcgctgga cctgcgcatg ctggtacgat tcgccaatag catccatccg
     3361 tgcctgcaga aaaccgatct tcgtggaggt gtgaagtgag aatccgtgtt ttggccgcgg
     3421 tactttcggt gctcgccgcg gtccccgtgt tcggtgccga gcgcccgttg acgctcgagg
     3481 aagcggtcgc attcgcgttg gaaaagaacg aaggcatctt catctcgcgc gagtccgcgg
     3541 ccgcggcgtc cgcgaacgcc tcgggcgcgc gcggggtcta caacccgctg ctcgtgctcg
     3601 acggctactg gcgcgagacc aagcagccgg tgaactccgc cttctcgggg gcaccgctgg
     3661 gcgagggcgc gccgaccacc gagtcgttgg aaggaagtgc cgtgctctca cagtacctgc
     3721 cgaccggggg gacattgtcc gcgcgcacga tcacggccaa gggcgagacc aacgggacct
     3781 acgacctgct ctcgcccgcg tatcaaacgc gtgtcggcgt cgagtttcgt cagccgctgt
     3841 tgcgcgattt cggcatggac gccgagcgcg tgtcgatcaa ggttgccgac gccgagcgca
     3901 agcgcggctc cgccgagttg cgcatagagg tcagcaacac cgtggccgag gtcgagcgcg
     3961 catactggaa cctggccgcc gcg
//
LOCUS       DASPGK010000228         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_295034, whole genome shotgun sequence.
ACCESSION   DASPGK010000228 DASPGK010000000
VERSION     DASPGK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295034"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /gene="tuf"
                     /locus_tag="VEC56_06220"
     CDS             complement(<1..1187)
                     /gene="tuf"
                     /locus_tag="VEC56_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXV13783.1"
                     /translation="MAREKFQRTKPHVNVGTIGHVDHGKTTLTAAITKVLARTGKAKF
                     VAYDQIDKAPEERARGITIATAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVNVPFMVVFLNKVDMVDDAELLDLVELEVRELLSS
                     YEFPGDDIPIIRGSALKAMEGEDAASGQCILDLMKAVDDYIPTPKREKDKPFLMPVED
                     VFSITGRGTVATGRIERGVVKVGDKVERVGIKDTADTVVTGVEMFRKLLDVAEAGDNV
                     GLLLRGLEKEDIERGMVLAAPKSITPHTKFKGKAYILTKEEGGRHTPFFNGYRPQFYF
                     RTTDVTGVATLPTGTEMVMPGDNVEMEIELITPIAMDLELRFAIREGGRTVGAGVVTE
                     IIK"
     gene            complement(1223..3331)
                     /gene="fusA"
                     /locus_tag="VEC56_06225"
     CDS             complement(1223..3331)
                     /gene="fusA"
                     /locus_tag="VEC56_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV13784.1"
                     /translation="MARKIPLDRVRNIGIMAHIDAGKTTMTERILFYTGKTHRLGEVH
                     EGTTIMDWMVQERERGITITSAATTASWKNSKGEEVRINIIDTPGHVDFTAEVERSLR
                     VLDGAIAVFCAVGGVEPQSETVWRQADKYGVPRIAFVNKMDRAGADFLNVVHMMKDRL
                     GANAVPIQLPIGTGELFNGIVDLVRMKAFFYHDTTKGMTYDEHDIPNDLVPLAKEYRA
                     KLVENLSDFDDELMSQFLDTGEGAQETLRRAMKKAMLESKFVPVMCGSAFKNKGVQRV
                     LDAVVHLLPSPADIPPITGHDLYSTETMTRKADEKEPFSALVFKIMTDPHVGKLTYIR
                     VYSGVLEQGDTVLNSTTSKKERIGRLMQMHADKREEIPAVYAGDICAVIGLRDARTGD
                     TLCDMKKAIVLERMHFPEPVLSVAIEPKTTADQEKLSEALVKLSDEDPTFQVRTDEET
                     GQTLIAGMGELHLEILVDRMMREFKVAANVGRPQVAYKEAFGKAVDAEGKFIKQSGGR
                     GQYGHVWIHAEPIVGGEDDFIFENKVVGGTVPREYVPAVEQGLRGALTAGVLAGYPVT
                     GVKIQLTDGSYHEVDSSEIAFKMAASIAFKEAMRRSNPVLIEPIMDVEVVVPKDYMGD
                     VIADLSSRRGKIGGMTQRAEAQVITARVPLAEMFGYATTLRSLTQGRAVYTMQFACYE
                     PAPKSVAEEVINKYQGKVAS"
     gene            complement(3538..4008)
                     /gene="rpsG"
                     /locus_tag="VEC56_06230"
     CDS             complement(3538..4008)
                     /gene="rpsG"
                     /locus_tag="VEC56_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXV13785.1"
                     /translation="MSRRNRSRTKEYAGDPKYNDVMVGRFVNYIMRKGKKSVAEAIVY
                     DCFDMIEQKTGQRGIEVFKKAIENVRPSLEVSSRRVGGATYQVPTEVRSTRRVALAMR
                     WISSYSRLRSEKTMAERLAGELMQASRSEGPSVKKKEDTHKMAEANKAFAHFRW"
     gene            complement(4036..4419)
                     /gene="rpsL"
                     /locus_tag="VEC56_06235"
     CDS             complement(4036..4419)
                     /gene="rpsL"
                     /locus_tag="VEC56_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXV13786.1"
                     /translation="MPTINQLIRRGRIEKPVRSKTPAMAACPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGVL
                     DTSGVEGRRQSRSKYGAKRPKAAAK"
BASE COUNT          756 a         1653 c         1384 g         1002 t
ORIGIN      
        1 tacttgatga tttcggtcac gacgccggcg ccgacggtcc ggccgccttc gcggatcgcg
       61 aaccgcaact ccaggtccat cgcgatcggc gtgatcaact cgatctccat ctccacgttg
      121 tctcccggca tcaccatctc ggtccccgtg gggagtgtcg ccacgcccgt cacgtccgtc
      181 gtccggaagt aaaactgcgg ccggtacccg ttgaaaaacg gcgtgtgccg cccgccctct
      241 tccttggtca ggatgtacgc cttccccttg aacttcgtgt ggggcgtaat gctcttgggc
      301 gccgccagca ccatcccacg ctcgatgtct tccttctcca gcccgcgcaa cagcaacccc
      361 acgttgtctc ccgcctccgc cacatccaac agcttgcgga acatctccac gcccgtgaca
      421 accgtgtccg ccgtgtcctt gatccccacg cgctccacct tgtcacccac cttcaccaca
      481 ccccgctcga ttcgtcccgt cgccaccgtc ccgcggcccg tgatcgaaaa cacgtcctcc
      541 accggcatca aaaacggctt gtccttctcc cgcttcggcg tcggaatgta gtcgtccacc
      601 gccttcatca ggtccagaat gcactgcccg ctcgccgcgt cctcgccctc catcgccttc
      661 agcgcactcc cgcgaatgat cggaatgtcg tcgcccggga actcgtagct cgagagcagc
      721 tcccgaacct ccagctctac caggtccaac aactccgcat cgtccaccat gtccaccttg
      781 ttcaggaaca ccaccatgaa cggcacgttc acctgccgcg ccagcagcac gtgctcccgc
      841 gtctgcggca tcggaccgtc cgcggcagaa accaccagaa tcgctccgtc catctgggcc
      901 gcgcccgtga tcatgttctt cacgtagtcc gcgtgtcccg gacagtccac gtgcgcgtag
      961 tgacgcttct ccgtctcgta ctccacgtgc gccgtcgcga ttgtgatccc gcgcgcgcgc
     1021 tcttccggag ccttgtcgat ctgatcgtag gccacgaact tcgccttccc cgtccgcgcc
     1081 aacaccttcg tgatcgccgc cgtcaacgtc gtcttcccgt ggtccacgtg cccaatcgtc
     1141 cccacgttga cgtgtggctt cgttctctgg aacttctccc tggccatgct cgatcctcct
     1201 tgagacagcg ccgggtcggc gactagctgg caaccttacc ctggtacttg ttgatgactt
     1261 cttcggcgac gctcttgggc gccggctcgt agcacgcgaa ttgcatcgta taaaccgcgc
     1321 gcccttgcgt cagcgagcgg agcgtggtcg cgtaaccaaa catctcggcc agcggcacgc
     1381 gggccgtgat cacttgcgcc tcggcgcgct gcgtcatgcc gccgatcttc ccgcgccgcg
     1441 agctcaagtc cgcaatcacg tcgcccatgt agtccttggg gacgacgact tcgacgtcca
     1501 tgatgggctc gatcaacacc gggttcgacc ggcgcatcgc ttctttgaac gcgatcgagg
     1561 cggccatctt gaacgcgatt tcgctggagt cgacctcgtg gtacgagccg tccgtgagct
     1621 ggatcttgac gcccgtcacc gggtagccgg cgagaacgcc ggccgtaagc gcgccgcgca
     1681 gaccctgctc caccgcgggt acgtactcgc gcggaaccgt tccgcccacg accttgttct
     1741 cgaagatgaa gtcgtcctcg ccgcccacga tgggctcggc gtgaatccac acgtgaccgt
     1801 actggccgcg gccgcccgac tgcttgatga acttgccttc ggcgtcgacc gccttgccga
     1861 acgcctcttt gtacgcgacc tgcgggcgcc cgacgttggc ggccaccttg aactcgcgca
     1921 tcatgcggtc gaccagaatc tcgaggtgca actcacccat gcctgcgatg agcgtctgtc
     1981 ccgtctcttc gtcggtgcga acctggaagg tcgggtcttc gtcggacaac ttgacgagcg
     2041 cttccgacag cttctcctgg tcggccgtgg tcttgggctc gatcgcgacc gatagtaccg
     2101 gctcggggaa atgcatccgc tcgagcacga tggccttttt catgtcgcac aatgtatcgc
     2161 cggtgcgcgc gtcgcgcaga ccgatgacgg cgcagatatc gcccgcatac acggcgggaa
     2221 tctcttcgcg cttatcggcg tgcatctgca tcaggcggcc gatgcgctcc ttcttactcg
     2281 tggtggagtt gagcaccgtg tcgccttgct cgagcacgcc gctgtaaacg cggatgtagg
     2341 tgagcttgcc gacgtgcgga tcggtcatga tcttgaacac gagcgccgag aagggctcct
     2401 tctcgtcggc tttgcgggtc atggtttcgg tgctgtagag gtcgtgtccg gtgatgggcg
     2461 ggatatcggc gggcgagggc agcaaatgca cgacagcgtc gagcacgcgc tgcacgccct
     2521 tgttcttgaa cgccgagccg cacatgacgg gcacgaactt gctctccagc atggccttct
     2581 tcatcgcgcg acggagcgtc tcctgcgcgc cctcccccgt gtcgaggaac tgcgacatca
     2641 actcgtcgtc gaagtcggag agattctcga ccagcttggc gcggtactcc ttggcgaggg
     2701 gcacgaggtc gttcggaatg tcgtgctcgt cgtacgtcat ccccttggtg gtgtcgtggt
     2761 agaagaacgc cttcatgcgc accaaatcga cgatcccgtt gaagagctcg cccgtgccga
     2821 tggggagctg gatgggaacc gcgttggcgc ccaggcgatc cttcatcatg tggaccacgt
     2881 tcaagaagtc cgcgcccgcg cggtccatct tgttgacgaa cgcgatgcgc ggaacgccgt
     2941 acttgtcggc ctgccgccac accgtctccg actgcggctc gacaccgccc accgcgcaga
     3001 acaccgcgat ggcgccgtcc aagacgcgca ggctgcgctc gacttcggcc gtgaagtcga
     3061 cgtgtcccgg cgtgtcgatg atgttgatgc gaacttcctc gcccttgctg ttcttccacg
     3121 acgccgtggt ggccgcggag gtaatggtga tgccgcgctc gcgctcctgg accatccagt
     3181 ccatgatagt ggtgccttca tggacttcgc ccaaccggtg cgtcttcccc gtgtaaaaga
     3241 ggatgcgctc cgtcatggtg gtcttgccgg catcgatgtg cgccatgatg ccgatgttgc
     3301 gtacgcgatc tagaggtatc ttcctggcca tttcttaccg attctttcgc gtcgatacga
     3361 cgcgcaatcg acgcccacgc ggggtcgcgc ggccgccttc cgttgcacta acttgagctg
     3421 cttgttacag cgaagcgccc gagtttcgac tatttgaggc ccgacaactc gtagactacc
     3481 ggtcgccggg gtaaaggttc gcgcgggcgt ttgcccgctt ccaaaccgtc cgtaggatta
     3541 ccagcggaag tgcgcgaaag ccttgttggc ctccgccatc ttgtgggtgt cttccttctt
     3601 cttgacggac gggccctccg aacgcgaggc ctgcatgagc tccccggcca gtcgctctgc
     3661 catcgtcttt tccgaccgca ggcgcgaata gctcgagatc caccgcatcg cgagggccac
     3721 tcgacgcgtc gaacgcacct cggtgggcac ctggtaggtg gcaccgccga cgcgacgcga
     3781 cgagacttcc aacgaggggc gcacgttctc gatggccttc ttgaagacct cgatgccgcg
     3841 ctgcccggtt ttctgctcga tcatgtcgaa gcaatcgtag acgatcgctt ccgcgaccga
     3901 cttcttcccc ttgcgcatga tgtaattgac gaagcgcccg accatcacgt cgttgtactt
     3961 ggggtcgccg gcgtattcct tcgtcctcga tcgattcctt ctcgacattg tccttcgtcc
     4021 ttgtggtacg cggcgctact tcgccgccgc cttcgggcgc ttggccccgt acttcgaccg
     4081 gctctggcga cgcccttcga cccccgacgt gtcgagcacg ccgcggatga tgtggtagcg
     4141 aacgcccggc aggtccttca cgcgaccgcc gcgaatcagc acgatcgagt gctcctggag
     4201 gttgtgccct tcgcccggga tgtaggccgt gacctcgatg ccgttggtca ggcgaacgcg
     4261 cgccaccttg cgaagcgccg agttgggctt cttgggggtc tgcgtgtaca cgcgggtgca
     4321 cacgccgcgc ttctgcggac acgcggccat ggccggggtc ttcgaacgga ccggcttctc
     4381 gatgcggccg cgtcgaatca actgattaat tgtcggcacg aatcctccgt ctgcgacgta
     4441 atgcgcccca tgcatccagg cggttgtgga taactcaacc gtcggcgccg atgccgggag
     4501 cgcaactgac tgtattatcg gtggttacca gacttccggg tcaggaagtg agggagccgc
     4561 gaactcggaa ctttagcggc tcgcgccaaa gctgtcaagc cccttttcgg ccgtaaccgc
     4621 ctacttctcc acactttcct tcccgctcgc cgtcatgggc tcgtcgtgaa ggtcgtcgac
     4681 ctcgtccagg gtcgcctccg gctccaccac gcccacgaac tcgtcgtcgt cgtcggccgg
     4741 cgccaactcg atgtggtcgt agtccttgac cccggttccg gccgggatca ggtgg
//
LOCUS       DASPGK010000229         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_762007, whole genome shotgun sequence.
ACCESSION   DASPGK010000229 DASPGK010000000
VERSION     DASPGK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762007"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..1184
                     /locus_tag="VEC56_06240"
     CDS             390..1184
                     /locus_tag="VEC56_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13787.1"
                     /translation="MMRELCAACAIVAVASLVGCNKEEAPVDPYVEATLREAVYGEVI
                     SPEFKYKIVDPEIVDGLGEFLVVKQSNMTEFIVGNGIAAKVDSLGDPEGITFNVVKQF
                     SPMVHFLCINVVTPTDSIVIASDKPIAFPRTSDAANFVPPADFVNGEMINFQWNNTDG
                     LRKMIGNKYAIEAHLVRVEEDSVTSWMLVGDSSQVVWGQVPKLRIRTPRPSLEIILRM
                     LERTQQNFVGGITYADIEPWDYRRKNYVCGTVDIGYLRFLDKVFSR"
     gene            1267..2268
                     /locus_tag="VEC56_06245"
     CDS             1267..2268
                     /locus_tag="VEC56_06245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV13788.1"
                     /translation="MKIAFVEPHLGLYGGIRRILEFANRFVDRGEDVTVFHPQGTACT
                     WMECRARVLPTSELFRASFDVVVFNDPPDYRLVRRARARLKVFYILELYEKDRLREWN
                     PKIVWPRKGRTLSLKRALQMPFLMVANATWIQRWLADNLSLESELVLGGVNRELFHPV
                     AGARAPSDAFVVLCSGDPREHKGTRTVTDAIERVRFAHPRVVLETYHGRGIPQSEMAA
                     CYARADLFVDAQWHAGWNNPVVEAMACGTPVVCSDIGGVADFAFHERTALVVAPRDVD
                     GFASAIARMIDSPALRAALAANALREVDRFDWDAAVERFLSLLYATRGAARAPAGTA"
     gene            2265..3401
                     /locus_tag="VEC56_06250"
     CDS             2265..3401
                     /locus_tag="VEC56_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXV13789.1"
                     /translation="MMKSFVLALCSLAVAFGAVEVVLRTTHAFNARVSWTEKDALIGW
                     RFTPGREYWCWEENDHPVSGRINALGWRDYERTRAKPAGVRRVAVLGDSYVEAFQVEL
                     DSTLAAVAERAAGARGGGSYQFMSFGRSGMSAAEERIVLERDVLPCAPDVVVLLFTPH
                     NDIADASPVTAPHRLRPFFAIHHDSLVLDTSFRTSRAFRFREFLNPLKQHSALISLVV
                     DRVNAKRASDNPRVDVPDMVGPTERFTRMHTLMTAHPDSQYAANYALHKRIVADMARM
                     CAQRGVVFALMSVPAVYQDERVEQLRAVDATFDPSFFERDFSAMADSSDFMVIPLFDR
                     FAERSRATGERLHWAHWNYHGHRVAGTALTGAVAGPAAGPGEAP"
BASE COUNT          619 a         1208 c         1213 g          654 t
ORIGIN      
        1 ttcgcgctgc ccgcggatca gaaacaccgc ggcgatcgcc aatacataga acgcgaccca
       61 ccagaagaag cgccggggcc gacgattgag agcctccatg gtcagcccac ccgctgggca
      121 caaggccggg tctcggacat cgaaacgtcg agaatggggg tttggcgaaa ggagcgatcg
      181 ccgtcatcga agttagtcca ccgcgcgccg taagtcaatc cccggaacca gatgccgatt
      241 gacaccgcca ggacccggtg ataaggtacc gcgctactgg tcgcccaaac cacccaccgg
      301 cgaccccgaa acacccatac ggcgcctgct cccgcggttc gcacggccgg ggaccgggcc
      361 atccctgatt gcctaggaga ggttcaccga tgatgcgaga gctgtgtgcg gcgtgcgcga
      421 tcgtcgcggt cgcgtccctc gtgggatgca ataaagagga agcgcccgtc gacccgtacg
      481 tggaggcgac gctgcgtgag gccgtctacg gcgaggtcat cagccccgag ttcaagtaca
      541 agatcgtcga cccggaaatc gtcgacggac ttggcgagtt cctggtcgtc aagcagagca
      601 acatgaccga gttcatcgtg ggcaacggga tcgccgccaa ggtcgattcg ctgggcgacc
      661 ccgagggcat caccttcaac gtcgtcaagc agttcagccc catggtgcac ttcctgtgta
      721 tcaacgtcgt gacgccgacc gactcgatcg tgatcgcgag cgacaagccg atcgcgtttc
      781 cacgcacgtc ggacgccgcc aacttcgtcc cgcccgcgga cttcgtcaac ggcgagatga
      841 tcaacttcca gtggaacaac accgatggtc ttcgaaagat gatcggcaac aagtacgcga
      901 tcgaagccca cctggtgcgt gtcgaggagg acagcgtcac gtcgtggatg ctggtcggtg
      961 attcctcgca ggtggtgtgg ggacaggtgc cgaagcttcg cattcgcacg ccgcggccga
     1021 gcctcgaaat catcctgcgc atgctcgagc gcacccagca gaacttcgtc ggcggcatca
     1081 cgtacgccga catcgagccg tgggattatc gccgcaagaa ctacgtgtgc ggcacggtcg
     1141 acatcggtta cctgcggttc ctggacaagg tgttctcgcg ctaggttggg agcatcgatg
     1201 gcttagcgaa ggggcgtcgc ggtgcggcgc cccttcgcct tttgtgcgcc tgcgagcccg
     1261 gcaatcatga agatcgcgtt cgtcgaaccc catctcggac tgtacggagg gatccgccgg
     1321 atcctcgagt tcgccaaccg cttcgtcgac cggggcgaag acgtcacagt gttccacccg
     1381 cagggaaccg cgtgcacgtg gatggagtgc cgcgcgcgcg tgcttcccac gtccgagttg
     1441 ttccgcgcct ccttcgacgt cgtggtcttc aacgatcccc ccgattatcg tttggttcgc
     1501 cgcgcccgcg cgcggctgaa ggtcttctat attctcgagc tctatgaaaa agaccgcctg
     1561 cgcgagtgga accccaagat cgtgtggccc cgcaaaggcc gcacgctctc gctcaagcgc
     1621 gcgctacaga tgccgttcct gatggtggcg aacgcgacct ggatccagcg ctggctcgcg
     1681 gacaacttgt cgctcgagag cgaactggtc ctgggcgggg tcaaccgcga gttgttccat
     1741 ccggtggctg gtgcgcgcgc gccgtcggac gcgttcgtcg tgctgtgctc gggcgatccg
     1801 cgcgagcaca aggggacccg aaccgtcacc gacgcgatcg agcgcgtgcg attcgcgcac
     1861 ccgcgcgtcg tgctggagac ctaccacggg cgcggcattc cgcagtcgga gatggcggcg
     1921 tgctacgcgc gcgccgacct cttcgtcgac gcgcagtggc acgcgggctg gaacaacccc
     1981 gtggtcgaag caatggcgtg cggtacgccg gtggtgtgca gcgacatcgg cggcgtggcc
     2041 gacttcgcgt tccacgagcg caccgcgctg gtggtggcac ctcgcgacgt cgacgggttc
     2101 gcgtccgcga tcgcgcgcat gatcgattcg cccgcgctgc gcgccgccct ggccgcgaac
     2161 gcgctgcgcg aggtggaccg cttcgactgg gacgccgcgg tggagcggtt cctctcgctc
     2221 ctgtacgcga cgcggggcgc cgcgcgagcg cccgcgggga ccgcgtgatg aaatcgttcg
     2281 tgctcgctct gtgctcgctc gcggtcgcgt tcggcgcggt cgaggtcgtt cttcggacga
     2341 cgcacgcgtt caacgcgcgc gtctcttgga ccgagaagga cgccctgatc ggctggcgct
     2401 tcaccccggg gcgcgagtac tggtgctggg aggagaacga ccaccccgtc tccgggcgca
     2461 tcaacgcgct cgggtggcgc gactacgagc gcacgcgcgc gaagccggcg ggcgtgcgcc
     2521 gggtggcggt gttgggcgac agctacgtgg aagcgttcca ggtcgagctg gactcgacgc
     2581 tcgcggcggt cgccgagcgc gctgccggtg cgcgcggcgg gggcagctac cagttcatga
     2641 gcttcgggcg ttccggtatg agcgccgcgg aagagcgcat cgtgctcgag cgcgacgtct
     2701 tgccgtgcgc gcccgacgtc gtcgtgctgc tgttcacgcc tcacaacgac atcgccgacg
     2761 cgagccccgt caccgcgccc catcgactgc gcccgttctt cgcgatccac cacgactcac
     2821 tcgtgctcga cacgtcgttt cgcacgtcgc gagcatttcg gtttcgcgaa ttcctgaatc
     2881 cgctcaagca gcactccgcg ttgatctcac tcgtggtcga tcgcgtcaac gccaagcgcg
     2941 cgagtgacaa tccgcgcgtg gacgttcccg acatggtggg accgacggag cggttcacgc
     3001 gcatgcacac gctcatgacc gcgcacccgg actcgcaata cgccgcgaac tacgcgctcc
     3061 acaagcgcat cgtcgccgac atggcgcgca tgtgcgcaca gcgcggcgtt gtgttcgcgc
     3121 tgatgtcggt accggcggtg taccaagacg agcgcgtcga gcagctgcgc gccgtcgatg
     3181 cgacgttcga tccgagcttc ttcgagcgcg acttctcggc gatggcggac agcagcgatt
     3241 tcatggtgat cccgctcttc gaccgcttcg ctgagcggtc gcgggcaacg ggcgagcgtc
     3301 tccattgggc gcactggaac taccacgggc accgggtggc ggggacggcc ctgaccgggg
     3361 ccgttgccgg tccggcggcg gggcccggcg aagcgccata accggatgag cggaatcggg
     3421 ttacgatttc actcggggtc ccgtgacgac cccgattttc ttcgaaaatt ccccgttgac
     3481 accccttgca aacccggggt atattgagcg gcacctcagc gaattcatcc cgatttcaat
     3541 ctcggatcga attcctaaag tctttcgggc acggggcact gtccccatgc tcgcatgttc
     3601 tttgacaatt cggatagtgt gcgtgagctc aagttgattt cgatttgagg ccgcgtgcgt
     3661 cgctgcttgc agcggcgcga atgcgcggtc ttgc
//
LOCUS       DASPGK010000230        12575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_411353, whole genome shotgun sequence.
ACCESSION   DASPGK010000230 DASPGK010000000
VERSION     DASPGK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411353"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VEC56_06255"
     CDS             <1..592
                     /locus_tag="VEC56_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXV13790.1"
                     /translation="DAENVRNSQYNVNNVTYQIVNLARTTLRNIIGTMTLKSANSERG
                     RINEELLKVLMKETHNWGIEIVRAELKQIDPPKDVQETMNKIVKAENEKIAAVDFATA
                     AETAADGQKRAAIKQAEGVRQANILEAEGQAEAIRLVNEAANRYFVGNAQLLKRLETM
                     ETSLKDNTQVVVPANSEIVNVIGDMAGVLPLDKGRI"
     gene            793..2097
                     /locus_tag="VEC56_06260"
     CDS             793..2097
                     /locus_tag="VEC56_06260"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HXV13791.1"
                     /translation="MKRERISNRLVLAWRVLAITLVVTVAVLITSAVGHAGTWAGKVS
                     TGANGAKTVTSPAKGFSESATIKLPELWRLGGDSEDEEEFFGVISDIDIDATGQVYLL
                     DSQLNEAKIYTKDGEFVRSIGREGEGPGEFRMPSSMFFTKDDKVAVIQMMPGKIVQLT
                     KDGQPSGEHAIPQAPDGGFQLIQGGDSRGGNIVLYMARQQFDQANNKWSRASFLTSID
                     PTGKQLAEYSKKENVISMASAEMNDASWDTFERRWEVGPDGKVYACNSFDNYEITVYD
                     KTGKIDKVITREYKHVARTADEKDFMNRMFSHWAKMIPNCKVVINDMNKDIDSIYLRD
                     DGSLWVLSSAGARNLAQGTLGTFDVFNPEGQFVKQVTLQGQGNPLEDLYVFEKDRLYV
                     VTSFLQAAMSAQGVQGLYDEADEPEPMAVICYKLEGDVLAAR"
     gene            2227..3573
                     /locus_tag="VEC56_06265"
     CDS             2227..3573
                     /locus_tag="VEC56_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV13792.1"
                     /translation="MFRILLVAIALLVGAVLGCGKKGDAQGAADSTATDSTSLANAKG
                     SMESDGENFGQSQDADASADTSGAKAERRERAINVNAARAIRADLVRPVIAEGQLRAR
                     HSAEIRAEVAGKIMRIGAEEGQAVKRGALLVKIDDREYEMALEESRARYLQALSLLTI
                     EDADVDTVVAQAKEVRDQFADLERLEKSGKITREERLAREIGLDVQALKEGKFRVEIA
                     AARSGVSVARADLERARLNLERTEVRAPFDGVVSGLTLSTGEQLMVNQVLFTLVDNVN
                     IEAEVGVLEADVGKIAPGGTAILAIPALGDTIPARVDVVSPQFNRETRTCQVLMRLKN
                     SDGRMRPGMFARALIAGERFEDRLLVPREAVLTREGRPLVFKVEGDRAKWLYVEVGAS
                     NDHMVEITGVLQGGTLEPDDRVVVSDHLTLAHDAKIKVRRTVASGDPWVTSAQGSE"
     gene            3570..6686
                     /locus_tag="VEC56_06270"
     CDS             3570..6686
                     /locus_tag="VEC56_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV13793.1"
                     /translation="MIRFAIHRPVAISMLFVALMLVGVVSFKRIPVDLLPSITYPRLT
                     VVTSYEDIPAEDLERLVTEPLEEVVTALSGVRRVVSRTREGVSMITVEYEWGTQMDFA
                     NLHLREAVDRVAFREDFPESAERPVILRWDPTSRPISILTLEGDAPIEQLTEFGEEVL
                     KPALQQVDGISKAEVVGGSIREILVEPDARKMAIYGITVEQIQSALVRSNVSFPGGKI
                     KQGPLQMSLRIDGEYGSIDEIAATDITRPGQSPVRIADVARVVDTIKEPEGVTLLGGH
                     PVVSFLIYKEPEANTIQVTDEVDRALGVLAEDYGDFQYQYVYRDADYVRASFSSLAWS
                     LVIGASLAFLVLFLFLNDVRSPLVVGVSIPVSLLITFVFLYLGKVKLNLMSLGGLSFA
                     AGLLVDNAIIVIENINRHVEEKLGAKKDFDPNDIETRRVIAQAAEQGTKEMRNAVIAC
                     TLTTVAVFLPVVYVPGIAGAFFRDQALTVTIALTVSIFAAMLLQPMLASRFLKPQRGA
                     RRGLFRWTDEGFRAFYRVYHGVLENALRRPRSMMAALVVGLIVCGFIGSRIPRSLMPE
                     RTSGDLRLELEYPTGTPLEETSVAAANLADWISTQAGVVNVFTQVGTTERTLAAMKDY
                     TAPNTARMRIIAESSRDAYETSRRLQEAVALKIAEMPGVRFAFREEGAGLAEVLATDE
                     APFSMGVIAERPEDAVTAAAEIVVALSGSRRLSDLQADRVLDTPNLVVHLDTEEILRA
                     GLDPDVIARDVRNRIAGVEATTFNEIEKRIDIAVRFPQDERRDLAGVLTSPVSVAGGE
                     MVPLKRFVTVDEEQPVRELTRANQRRMVTVSGQVADGSIDDAWREANAVMERLGLPAD
                     VKIVQSGERAEMNRSFRDLLWAFLLATVLVYMIIAAQFESLVDPLHIAMTIPIGAAGT
                     VIALLATGTSVNILSLIGLITLLGIAVDDAIVKTDTIRQLRAGGMDGWKAIHEGCRLR
                     ARPIIMNSATAILAMVPLAIGLGGGAQLQRPLALTIIGGLTLTTALTLLYTPVMYMLC
                     HRIRRPEA"
     gene            6683..10009
                     /locus_tag="VEC56_06275"
     CDS             6683..10009
                     /locus_tag="VEC56_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV13794.1"
                     /translation="MTKFCVRFPVTTWMIFVSFVVLGAYSIPRLKIEAVPDVNLPTLT
                     VSTGWNGASPQAVQRSITIPIEEAVQDVHGVEKLESTSRAGNSTVEISFRRDVEIDFA
                     RLDLNEQLGDVRRNLPLGASQPQILPFVPEEFRTEDFFTFSLESSLSPNELRELAETW
                     VIPQLIAINGVADAEVLGGARPLVKIVLDRRLLELYGIRADEVFRGVDRLDEFEGAGT
                     VREHGLERLVALRRPVSLRQLSEATVARRGGQSFTLGMLGEARADFEDPVYFVRANGK
                     NVVQVSVDKRSGSNAVGVSRDLRKALPSIRANVPAPIDFHIDEDQGQELEDKLRDLIY
                     RSFVILAILFVMMVVSVRRVKLTAIVTASIVFATLISLSMFYFLRISVNFITISGLAV
                     SFGMIVDNSILVLESIHRRLDALERANALGLSRRAKLEVAYHAILDGTRDIANPALAT
                     TLTSIVVFVSFVFLSGRLALYYIPLAVSVTTAMVASLFVAFGWVPMVLHRVWARPLVS
                     RLPDGPRDVVDPDELDRLIAEPPATEDKVTRIDRFYHGLQRGWYVVVPLALAIFVGAW
                     WVYDTKVVKGGFWRFPEEEELFLYLEMPSGTDLELTSETLRDFEEELLPAPEGVRMSS
                     RAFGNQAIMRVEFEEELRTTPLPFYWRELLIAKADVTGGSSVFIRGFSDRPYFKGAFG
                     GSALNSLVKITGYNSKRLNEIADGALGRIEKNRRVRNARVTTGAQWERIRQEEMVIRI
                     KRERLAAHDLTVTDLTAQVRRMLGVDIPWTMLIEGDQERVQLSYMDAENISYADIAQL
                     VVRNPAGEQVRLGELIELETREVSRSITRENQKYTTHVNWEYLGTDQMRQKYIKDVLA
                     GIQLPYGYKAEEARQEFLTQEEEEEIGLALWLAAAFIFIVLAAMLESLSLPFLILLAI
                     PMSLVGVFVIFWLTHSVFDSSARIGLVLLFGVAVNNAILLVTRYRVEATLILKSKLGR
                     DPELSTSLFPPLRRHVGGSDLWVLPKEERVELLKRAVARGSRVRLRSILLVSGTTIVG
                     LAPLLVHMNETADKDIWENLSLATIGGLTSSTILLLLISPAVYTGCIRLSWQLRRFTA
                     WVKRKASRRARDLAPAPEIG"
     gene            10220..11908
                     /locus_tag="VEC56_06280"
     CDS             10220..11908
                     /locus_tag="VEC56_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13795.1"
                     /translation="MAPVAQGASLSDVWGPSADNLFATATGGVVLRYDGTTWRKTITP
                     TTANLNAIWGTSASHVVAVGDDGVVLVYNGSTWTAQDSGTGLYLLDVWGTSPNDVFAV
                     GLDRTVLHYNGVSWTTMTTEQFGVALNGVWGSSASNVYATGLGRNLFHYDGTSWSVEA
                     TPATFALSAVWGTGPDEIFAVGGNGAAIRFDGVSWSPIDVGSSLFSSDLWGVSGSDVY
                     AVGQNGSVFHWDGLAWDPMVNSSIQELDGVFGVDGRVFAVGQYGTILELSGSEWTHAP
                     EGLTFDLEDVWADAAGNAFAVGDHGTVLRLQDGVWRRMTSGTTNHLRSLHGNSSSDLV
                     AVGRGGVVLRFDGSSWTSSSEGPGFDLHDVYVLPTGEAYAVGAGGLILRSQGGAWIDA
                     SVPSVTDSLLSVWGASASNVYVVGAQSRAFNWNGLQWKLVTIDLSRVNNYHAIHGSSS
                     SDIFVGAEFLQAASLAGEAPLHAGGLIYHWNGTSWTIPYQDPIHDVLSVWAASTESGF
                     ATGDAATILVRSGSGWVRISDLSNLPFNMTSVWGASASDVIVVGDNGTIARYSR"
     gene            11910..>12575
                     /locus_tag="VEC56_06285"
     CDS             11910..>12575
                     /locus_tag="VEC56_06285"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HXV13796.1"
                     /translation="MRFASVAVTSSNVDVLLRDAGKAVLDRLGEHADVAFVFVNAALA
                     PRAETLAANLKRAIGARVLVGCTGEGVIGPAHEIEDQPALAVVAAQLPGVEIDAFAIP
                     KRDLLKIALNPGSLCSFHDVPFGTKFAVILADPFSTPMDGVLKAINQEYDGIPVIGGM
                     ASGARQPGETVLFLNETVVREGLVGVAFAGAITVDVIVSQGCRPVGPVFEVTEATYNV
                     IDSL"
BASE COUNT         2188 a         4026 c         4169 g         2192 t
ORIGIN      
        1 cgacgccgag aacgtccgca attcgcagta caacgtgaac aacgtgacgt accagatcgt
       61 caatctggcg cgcacgacgc tgcgcaacat catcggtacc atgacgctca agtcggccaa
      121 cagcgagcgc gggcgcatca acgaagaact gctcaaggtc ttgatgaagg agacgcacaa
      181 ctggggcatc gagatcgtgc gcgccgagct caagcagatc gacccgccca aagacgtcca
      241 ggagaccatg aacaagatcg tcaaggcgga gaacgagaag atcgcggcgg tcgacttcgc
      301 gaccgcggcc gagaccgcgg ccgacggcca gaagcgcgcg gccatcaagc aggccgaggg
      361 ggtccgccag gccaacatcc tggaggccga agggcaggcg gaggccatcc gtttggtcaa
      421 cgaggcggcc aaccggtact tcgtcgggaa cgcccagctc ctgaagcggc tcgaaaccat
      481 ggaaacctcc ctaaaagaca acacccaggt cgtggtgccg gccaattcgg agatcgtcaa
      541 tgtgatcggt gacatggccg gggtgctgcc gctcgacaag gggcggatct aggtctcggt
      601 tggtccggtt gccccagggg catcaagggg ttacaatggt cgccgccgtc gggtgcctcc
      661 cgaatagtcc gcgggccgcc gggtagatgg ggcaacgccg ggcgtgaatt gatggaaccc
      721 ccgcccggtg cgcatcgtag gtaccgcatc caccggtttg catacccatt tcctgaagga
      781 gaaccgatcc ccatgaagcg tgagcgcata tcgaaccggc tcgtcctggc gtggcgagtc
      841 ctcgcgataa cgttggtggt gaccgtcgcc gttctgatca cgagcgcggt cggtcacgcc
      901 ggtacctggg cgggcaaggt ctcaacgggc gccaatggtg ccaagacggt aactagcccg
      961 gccaagggct tctcggaatc ggccaccatc aagctgcccg aattgtggcg cctgggcggt
     1021 gactccgagg acgaggagga gttcttcggc gtcatcagcg atatcgacat cgacgccacc
     1081 ggtcaggtct acctgctcga cagccagctc aacgaggcca agatctatac caaggacggc
     1141 gagttcgtcc gctcgatcgg ccgcgagggc gaaggacccg gcgagttccg catgccctcg
     1201 tcgatgttct tcaccaagga cgacaaggtt gcggtcatcc agatgatgcc gggcaagatc
     1261 gtccagctca ccaaggacgg gcagccgtcc ggcgagcacg ccatcccgca agcccccgac
     1321 ggcggattcc agctgatcca gggcggagac tcgcgcggtg ggaacatcgt cctgtacatg
     1381 gcgcgccagc agttcgacca agccaacaac aagtggtcgc gtgccagctt cctgacctcc
     1441 atcgatccca ccggcaagca gttggccgag tactcgaaga aggagaacgt catcagcatg
     1501 gcgtcggcgg agatgaacga cgcctcctgg gacaccttcg agcggcgctg ggaagtcggc
     1561 cccgacggca aggtgtacgc gtgcaactcg ttcgacaact acgagatcac ggtgtacgac
     1621 aagaccggca agatcgacaa ggtgatcacc cgcgagtaca aacacgtggc gcggacggcg
     1681 gacgagaagg acttcatgaa ccgcatgttc agccattggg ccaagatgat tcccaactgc
     1741 aaggtcgtca ttaacgatat gaacaaggac atcgacagca tctacctgcg cgacgacggt
     1801 tcgctgtggg tgctctcgag cgcgggcgcg cgcaacctgg cccagggaac gctgggcacc
     1861 ttcgacgtgt tcaaccccga gggtcagttc gtcaagcagg tgacactgca gggacaaggc
     1921 aacccgctcg aggacctgta cgtgttcgaa aaggaccgct tgtacgtggt gacgagcttc
     1981 ttgcaggccg cgatgtcggc gcagggcgtc cagggtctct acgacgaggc cgacgagccg
     2041 gagccgatgg cggtcatctg ctacaagctc gagggcgacg ttctcgccgc ccgctagaaa
     2101 cggcagcatg cgtcggcggg tggaccgccg acacgccaac tcgtgtaact acgacgccgg
     2161 cgtcgcgcgg gatgtgcgcg acgccggcgt cgctccaagt cgacccagag gagaggacgc
     2221 cccgtgatgt tccgaattct gctcgtcgcg atcgcgctgc tggtgggcgc ggtcctcggg
     2281 tgtgggaaga agggcgacgc gcaaggcgcc gccgattcca ccgccaccga ctcgacttcg
     2341 ctcgccaacg ccaagggctc catggagagc gacggcgaaa acttcggaca gagtcaagac
     2401 gccgacgcgt cggcggatac cagcggtgcc aaggccgagc gcagggagcg cgcgatcaac
     2461 gtcaacgcgg cccgcgcgat ccgcgcggac ttggtgcggc cggtcatcgc ggaaggtcaa
     2521 ctgcgcgcgc gccactccgc ggagatccgc gccgaggtgg cgggcaagat catgcgcatc
     2581 ggggccgagg aaggccaggc cgtcaagcgc ggcgcgctcc tggtcaagat cgacgaccgc
     2641 gagtacgaga tggctctcga ggagtcacgt gcacgttatc tgcaggcgct gagtctcctc
     2701 accatcgagg acgccgacgt cgacacggtg gtggcgcagg ccaaggaagt gcgcgaccag
     2761 ttcgccgacc tcgaacgctt ggagaagagc ggcaagatca cgcgcgaaga aaggctcgcg
     2821 cgcgagatcg gcctcgacgt gcaggcgctc aaggaaggga agttccgggt cgagatcgcg
     2881 gccgcgcgca gcggcgtgtc ggtcgcgcgc gccgatctgg aacgcgcgcg cttgaacctg
     2941 gagcgaaccg aggtccgcgc gcccttcgac ggtgtggtga gcgggctcac gttgagtacg
     3001 ggtgagcagc tcatggtcaa ccaggtgctc ttcacgctgg tcgacaacgt caacatcgag
     3061 gcggaggtgg gcgtgctcga agccgacgtg ggtaagatcg cgcccggcgg taccgccatc
     3121 ctcgccattc ccgccctggg cgacaccatc cccgcgcgcg tggacgtcgt cagcccgcag
     3181 ttcaaccgcg agacgcgcac ctgccaggtc ctcatgcgcc tcaagaacag cgacggccgc
     3241 atgcgccccg gcatgttcgc gcgtgcgctg atcgcgggcg agcgcttcga ggaccgcttg
     3301 ctggtcccgc gcgaagccgt gctcacgcgc gaagggcgcc cgttggtatt caaggtggaa
     3361 ggcgaccgcg ccaagtggct ctacgtcgag gtcggggcca gcaacgacca catggtcgag
     3421 atcaccggcg tgctgcaagg cggcaccttg gagcccgacg accgtgtggt cgtctccgac
     3481 cacttgacgc tcgcgcacga cgccaagatc aaggtgcgcc ggacggtggc gtccggcgac
     3541 ccgtgggtca cttcggcgca gggcagcgaa tgattcgttt cgcgatccac cgtcccgtcg
     3601 ccatcagcat gctgttcgtc gcgctgatgc tggtgggcgt ggtgagcttc aagcgcatcc
     3661 cggtcgatct cctcccgtcg atcacgtacc cgcgcctcac cgtcgtcact tcgtacgagg
     3721 acatcccagc cgaggacctc gagcgcctcg tcaccgagcc cctcgaggaa gtcgtcaccg
     3781 cgctctccgg cgtacgccgc gtggtatcgc gcacgcgcga gggcgtttcc atgatcacgg
     3841 tcgagtacga gtggggcaca cagatggact tcgccaatct ccatctgcgc gaggccgtcg
     3901 accgcgtggc gtttcgcgag gactttcccg agagcgcgga gcgtcccgta atcctgcgct
     3961 gggatcccac ctcgcgtccc attagcattc tcaccctgga gggcgacgcg cccatcgagc
     4021 agctgaccga gttcggcgag gaagtcttga agcccgcgct gcagcaggtc gacggcatca
     4081 gcaaggcgga agtggtgggc ggctccattc gcgagatcct ggtggagccc gacgcgcgca
     4141 agatggcgat ctacggcatc accgtcgagc agatccagag cgccctggtg cgcagcaacg
     4201 tgtcgttccc gggcggcaag atcaaacagg ggccgctgca gatgagcctg cgcatcgacg
     4261 gcgagtacgg ctcgatcgac gagatcgccg ccaccgacat tacccgaccc ggccagtcgc
     4321 cggtgcgcat cgccgacgtc gcgcgcgtcg tcgataccat caaggagccg gaaggggtga
     4381 cactcttggg cggccacccg gtggtgtcgt ttctcattta taaggagccc gaggccaaca
     4441 ccatacaagt gaccgacgag gtcgaccgcg cgctgggcgt cctggccgag gattacggcg
     4501 atttccagta ccagtacgtc taccgcgacg ccgactacgt acgggcttcg ttctcgagcc
     4561 tggcctggtc gctcgtgatc ggtgcgtcgc tggcgtttct ggtgctgttc ctatttttga
     4621 acgacgtgcg tagcccgctc gtcgtgggcg tgtcgattcc cgtctcgctc ctgatcacct
     4681 tcgtgttctt gtacctgggc aaggtcaaac tcaacctgat gagcctgggc ggactctcgt
     4741 tcgcggccgg cctgctcgtc gacaatgcca tcatcgtgat cgagaacatc aaccgtcacg
     4801 tcgaggagaa gctgggagcg aaaaaggatt tcgaccccaa cgacatcgag acgcgccggg
     4861 tcatcgcgca ggcggccgag cagggcacca aggagatgcg caacgccgtc atcgcgtgca
     4921 cgctcaccac ggtcgcggtg ttcctgccgg tagtgtacgt gccgggtatc gcgggggctt
     4981 tcttccgcga ccaggccctg accgtcacca tcgcgctcac ggtatcaatc ttcgccgcca
     5041 tgctcttgca gcccatgctg gcttcgcgct tcctcaagcc ccagcgcggc gccaggcgcg
     5101 gtctgtttcg atggaccgac gagggtttcc gcgccttcta ccgcgtctac cacggtgtct
     5161 tggaaaacgc gctgcggcgt cccaggtcga tgatggcggc actggtagtg ggtttgatcg
     5221 tatgcgggtt cattggatca cgcatcccgc gcagcttgat gcccgagcgc acgagcggtg
     5281 atctgcggct cgagctcgag tacccgaccg gcactccgct ggaagaaacg tcggtggctg
     5341 ccgccaatct ggccgattgg atcagcacgc aggccggcgt ggtcaacgtg ttcacccagg
     5401 ttggaaccac cgagcgcaca ctggcggcga tgaaggacta caccgcgccc aacaccgcgc
     5461 gcatgcgcat catcgcggaa tcgtcgcgcg acgcgtacga aacgtcacgc cgtctgcaag
     5521 aggcggtggc actcaagatc gccgagatgc ccggcgtgcg cttcgcgttt cgcgaggaag
     5581 gcgccgggct cgccgaggtg ctcgcgaccg acgaagcccc cttcagcatg ggtgtcatcg
     5641 ccgagcgtcc cgaagacgcg gtgacggcgg cggcggaaat cgtcgtggcg ttgtccggct
     5701 cacggcgctt gagcgacctc caggccgacc gcgtactcga cacgcccaac ttggtcgttc
     5761 acctggacac cgaggagatc ttgcgagccg ggctcgaccc cgacgtgatc gcgcgcgacg
     5821 tgcgcaaccg catcgccggc gtcgaagcca ccacgttcaa tgagatcgag aagcgcatcg
     5881 acatcgcggt gcgctttccc caggacgagc gacgcgatct ggccggcgtg ctgacgtcgc
     5941 cggtgtcggt ggcgggcggg gagatggtgc cgctcaagcg cttcgtcacc gtcgacgagg
     6001 agcagccggt gcgcgaactc acgcgagcga atcagcgccg catggtgacg gtgtcgggcc
     6061 aggtcgcgga cggctccatc gacgacgcct ggcgcgaggc aaacgccgtc atggaacgcc
     6121 tcggccttcc cgccgacgtc aagatcgtgc agagcggcga gcgcgccgag atgaaccgca
     6181 gcttccgcga cctcctgtgg gcgttcttgc tcgcgaccgt gctggtgtac atgatcatcg
     6241 cggcccagtt tgaatcgctg gtcgatccgc tccacatcgc gatgacgatt ccgatcggcg
     6301 cggccggcac cgtgatcgcg ctgctggcca cggggacgtc ggtcaacatt ctttcgttga
     6361 tcggattgat cacactgctc ggcatcgcgg tcgacgacgc catcgtcaag accgacacga
     6421 ttcgccagtt acgcgcgggc ggcatggacg ggtggaaggc catccacgaa gggtgccggc
     6481 tgcgagcacg ccccatcatc atgaacagcg ccaccgcgat tctcgccatg gtgccgctcg
     6541 ccatcgggct gggtggcggc gcgcagctcc agcgcccgct ggcgctgacc atcatcggcg
     6601 gcttgacgct gaccacggct ctgacgctcc tgtacacgcc ggtgatgtac atgctctgcc
     6661 accgcatccg gaggcccgag gcatgaccaa gttctgcgtg cgcttcccgg tgacgacgtg
     6721 gatgatcttc gtgtcgttcg tcgtgctggg cgcgtactcg atcccgcgcc tcaagatcga
     6781 ggccgtaccc gacgtcaact tgcccacgct gacggtcagc accggctgga acggcgcctc
     6841 gccgcaagcc gtgcagcgct cgatcacgat ccccatcgaa gaggcggtgc aggacgtcca
     6901 cggggtcgag aagctggagt ccaccagccg cgccggcaat tccaccgtcg agatatcgtt
     6961 ccgccgcgac gtcgagatcg acttcgcgcg tctggacttg aacgaacagc tgggcgacgt
     7021 gcggcgcaac ctcccgctgg gcgcgtcgca gccgcagatc ctccccttcg tccccgagga
     7081 gtttcgcacc gaggacttct tcacattcag cttggaatcg tcgctgtcgc ccaacgaatt
     7141 gcgcgaacta gccgagacat gggtgattcc gcagttgatc gcgatcaacg gtgtcgccga
     7201 cgccgaggtc ctgggcggtg cgcggccgct ggtgaagatc gtcctcgacc ggcgcttgct
     7261 cgagctctac ggcatccgcg ccgatgaggt gtttcgcggc gtggaccgcc tcgacgagtt
     7321 cgagggcgca ggaaccgtcc gcgagcacgg actcgaacgc ctggtcgcgc tgcgtcgccc
     7381 ggtgagcctg cggcagctgt cggaggcgac ggtggcgcgt cgcggcgggc agagcttcac
     7441 actcggcatg ctgggcgagg cgcgcgccga cttcgaagac cccgtctact tcgtgcgcgc
     7501 caacgggaag aacgtggtgc aggtttcggt cgacaaacgc agcggttcca acgcggtggg
     7561 cgtgagccgc gacctgcgca aggcgctgcc gtcgattcgc gccaacgtac ccgcccccat
     7621 cgacttccac atcgacgagg accagggcca ggaactcgag gacaagcttc gcgatttgat
     7681 ttatcggtcg ttcgtcattc tcgccatcct gttcgtgatg atggtcgtga gcgtgcggcg
     7741 cgtgaagctc accgcgatcg tgacggcatc gatcgtgttc gccacgttga tctcactctc
     7801 gatgttctat tttctgcgca tctcggtgaa cttcatcacc atcagcgggc tcgcggtgag
     7861 cttcggcatg atcgtcgaca acagcatcct ggtgctcgaa tccatccacc gccgcctgga
     7921 cgcgctcgag cgcgccaacg ccctcggact ctcgcggcgc gcgaagctgg aagtcgccta
     7981 ccatgcaatc ctagacggca cgcgcgacat cgccaatccc gcgctcgcga cgacactcac
     8041 gtccatcgtg gttttcgtga gcttcgtgtt cctttccggt cgtctggcgc tgtactacat
     8101 cccgctcgcg gtctcggtga ccaccgcgat ggtcgcgtcg ctgttcgtcg cgttcggttg
     8161 ggtgcccatg gtgctgcatc gcgtgtgggc gcgaccgctg gtgagccggc tgcccgacgg
     8221 cccgcgcgac gtcgtcgacc ccgacgagct ggaccgcctg atcgcggagc cccccgcgac
     8281 cgaggataag gtcacgcgga tcgatcgctt ctatcacggc ttgcagcgcg gctggtatgt
     8341 ggtggtcccg ctcgctctcg caatcttcgt cggcgcctgg tgggtgtacg acaccaaggt
     8401 ggtcaagggc ggcttctggc gtttccccga agaggaggag ctgtttctct acctcgagat
     8461 gccgtccggc accgacctgg agctgacgtc ggaaacgctg cgcgacttcg aggaggagtt
     8521 gctcccggca cccgagggcg tgcgcatgag ctcgcgcgcg ttcggcaacc aggccatcat
     8581 gcgcgtcgaa ttcgaagaag agctgcgcac gaccccgctt ccgttctact ggcgcgagct
     8641 attgatcgcc aaggccgacg tcaccggcgg gtcgagcgtg ttcattcgcg gcttctccga
     8701 tcgaccgtac ttcaagggcg ccttcggcgg gtcggcgttg aattcgttgg ttaagatcac
     8761 cggctacaat tcgaaacgac tgaacgagat tgccgacggc gcgctcggtc gcatcgagaa
     8821 gaaccgccgc gtgcgcaacg cgcgtgtcac cacgggcgcg caatgggagc gcatccgcca
     8881 ggaagagatg gtgattcgca tcaaacgcga gcgcctggcc gcgcacgacc tcacggtcac
     8941 cgatctcacc gcccaggtgc ggcgcatgct gggcgtcgat atcccctgga ccatgctgat
     9001 cgagggcgac caggaacgcg tccaattgtc ctacatggac gcggaaaaca tctcctacgc
     9061 cgacatcgca cagctggtgg tgcgcaaccc ggccggcgag caggtccgcc tgggcgaatt
     9121 gatcgaattg gagacgcgcg aggtttcgcg ctcgatcaca cgcgagaacc agaagtacac
     9181 cacccacgtc aactgggagt atctgggcac cgaccagatg cgtcagaagt acatcaagga
     9241 cgtgctcgcg ggcatccagc tcccgtacgg ctacaaagcc gaagaggcgc gccaggagtt
     9301 cctcacccaa gaagaggaag aggaaatagg actcgcgctt tggctcgcgg cggcgttcat
     9361 cttcatcgtg ctggccgcga tgctggagag cctgtccctc ccgttcttga tccttcttgc
     9421 gatcccgatg tcgctggtgg gcgttttcgt catcttttgg ctcacccact cggtgttcga
     9481 ttcgtccgca cgcatcggcc tggtgctgct gttcggggtc gcggtcaaca acgcgattct
     9541 gctcgtcacc cgctaccgcg tcgaagcgac gttgatcctc aagtcgaagc tcgggcgcga
     9601 tcccgagctg tccacgtcgc tgttcccgcc gctgcgcagg cacgtgggag gcagcgactt
     9661 gtgggttctg cccaaggaag aacgcgtcga gctgctcaag cgggcggtcg cgcgcggatc
     9721 ccgcgttcgc ctgcgttcga tcctcctggt cagcgggacc accatcgtcg ggttggcgcc
     9781 gctgctggtc cacatgaatg agaccgccga caaggacatc tgggaaaatc tctcgctcgc
     9841 gaccatcggc gggctgacgt cgagtacgat actcctcctc ctgatcagcc cggccgtcta
     9901 cacggggtgc attcgactgt cgtggcagtt gcgacgcttc accgcgtggg tgaagcgaaa
     9961 ggcgtcgcgt cgcgcgcgcg atctcgcccc cgcgcccgag atcggttgag cgcgttgcgc
    10021 ggcggcagcg tttcgtagta aattgatacg cgaacgcccc gctgtcggag accccgtgcc
    10081 tttccattca tcctcgctcg cagactcggt gcttcgttcg ttggccgcgg cgaccatcct
    10141 ggccgtgctt gggtgctcga gttcccccga ttcctctccc ccggtcaatc ccgttcccac
    10201 cgcggcccga tggcggtgga tggcgccggt cgcccaaggg gcgtcgctct ccgacgtgtg
    10261 gggcccctcg gccgacaatc tctttgcgac cgcgaccggc ggcgtcgtgt tgcgctacga
    10321 cggtaccacc tggcgcaaga cgatcacacc caccaccgcg aacctcaacg cgatctgggg
    10381 aacgtcggcc agccacgtcg tggcagtcgg cgacgacggt gtcgtgctgg tctacaacgg
    10441 atcgacctgg accgcgcaag acagcggaac cggtttgtat ctcctcgacg tgtggggcac
    10501 gtcgcccaat gacgttttcg cggtcgggtt ggaccggacg gtactccatt acaacggcgt
    10561 atcctggacc acgatgacca cggaacagtt cggtgtcgcg ctcaacggcg tgtggggaag
    10621 ctcggcgagc aatgtgtacg cgaccgggct ggggcggaat ctgtttcact acgacgggac
    10681 atcgtggagc gtggaggcga ccccggccac cttcgcgctg agtgccgtct gggggaccgg
    10741 acccgatgag atcttcgcgg tcggcggcaa cggcgcggcg attcgtttcg acggcgtgtc
    10801 gtggtcgccg atcgacgtgg gatcgtcgct gttctcgagt gatctgtggg gtgtgagcgg
    10861 cagcgacgtg tacgcggtcg ggcagaacgg aagcgtcttc cattgggacg ggctggcgtg
    10921 ggatccgatg gtgaactcca gcatccaaga gctggacggc gttttcggcg tcgacgggcg
    10981 cgtgttcgcg gtggggcagt acgggacgat tctggagtta tcggggtcgg agtggacgca
    11041 cgcgccggag ggcctcacct tcgacctgga agacgtgtgg gcggatgccg ccgggaacgc
    11101 cttcgcggtt ggcgatcacg gcaccgtgct gcgcctgcaa gacggggtgt ggcggcggat
    11161 gacgagcggg acgacgaacc acctgcgctc gctgcacggc aactcgtcga gcgatctcgt
    11221 cgcggtcggg cggggcggcg tcgtgcttcg cttcgatggc tcgtcgtgga cgtcttcgag
    11281 cgaaggcccg ggtttcgatc tccacgacgt gtacgtgcta ccgaccggcg aggcgtatgc
    11341 ggtgggcgcg ggcggcctga ttctccgctc ccaaggcggc gcatggatcg acgcgtcggt
    11401 acccagcgtc accgactcct tgctgagcgt gtggggggcg tcggcgagca acgtgtacgt
    11461 ggtgggggcg cagtcgcgcg cgttcaactg gaacggtctc cagtggaagc tcgtgaccat
    11521 cgacctctct cgcgtcaaca actaccacgc tatccacggt tcctcgagca gcgacatctt
    11581 cgtgggcgcg gagttcctgc aggcggcttc gctggcggga gaggcgccgc tgcacgcagg
    11641 cgggctcatc tatcactgga acgggacgtc gtggacgatc ccgtaccaag accccatcca
    11701 cgacgtgctc tcggtgtggg cggcatcgac cgaaagcggg tttgccaccg gcgacgccgc
    11761 gacgatcctg gttcgatcgg gaagcggttg ggtgcgtatt tccgacttga gcaatttgcc
    11821 cttcaacatg acctcggtgt ggggggcttc ggccagcgac gtaatcgtgg tcggtgacaa
    11881 cggcacgatt gcgcgttata gtcggtgata tgcgcttcgc gtccgtagcc gtcaccagct
    11941 ccaacgtcga cgtcctcttg cgcgacgccg gcaaggccgt gctcgatcgt ttgggtgagc
    12001 acgccgatgt cgccttcgtg ttcgtcaacg cggcgctcgc gccgcgcgcg gagacgctgg
    12061 ctgcgaacct caagcgtgcc atcggtgcgc gcgtgctggt cggttgcacc ggagagggtg
    12121 tgatcggtcc ggctcatgag atcgaggatc agcccgcgct ggcggtggtg gccgcccaac
    12181 tgcccggtgt cgagatcgat gcgttcgcga ttcccaagcg cgacctcctg aagatcgcgc
    12241 tcaatccggg gtcgctgtgc tcgttccacg acgttccctt tggcaccaag ttcgcggtga
    12301 tactggccga tccgttttcg acgcccatgg acggggtgct caaggccatc aatcaggagt
    12361 atgacggtat cccggtcatc ggcggcatgg ccagcggtgc gcgccagccg ggcgagacgg
    12421 tcctgttcct caacgagacc gtggtgcgcg aggggttggt gggggtggcg ttcgcgggcg
    12481 ccatcacggt cgacgtcatc gtgtcgcagg ggtgccgccc ggtggggccg gtgttcgaag
    12541 taaccgaagc gacctacaac gtgatcgatt ctctg
//
LOCUS       DASPGK010000231         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_59979, whole genome shotgun sequence.
ACCESSION   DASPGK010000231 DASPGK010000000
VERSION     DASPGK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59979"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VEC56_06290"
     CDS             <1..473
                     /locus_tag="VEC56_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13797.1"
                     /translation="LDEVETTSRHLREIAVSLSGDGRLQEGVNDFAEASKNVRGMTER
                     NKDRLDRALMRFERSATMLDSLITTRYPAIDSSLAAIGRAGGKAETTVDNLAEVSKDL
                     REITAKLRAGEGSAGRLLNDDTFITRLESTVAELDSLVADIQRNPGRYVKFSLF"
     gene            478..1176
                     /gene="ispD"
                     /locus_tag="VEC56_06295"
     CDS             478..1176
                     /gene="ispD"
                     /locus_tag="VEC56_06295"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXV13798.1"
                     /translation="MKAAALIMAGGSGERFVAGSEREAKQLAPIAGRAMIAWSAEALA
                     RSTRVVRLIVALPGGMEAGVRAVLPPDAVRKMHAFVEGGATRQESVFNALRALPDDVT
                     HVLIHDAARPCVSSQLIERVLDALETRDAVVPATAATDTLVRDYEGRVDAIIDRVHVA
                     GVQTPQAFRRELILRAHREARARGFHSSDDGSLVLALGETVHTVPGERTNIKVTFRDD
                     VAIAEAILKNGVRT"
     gene            1173..1691
                     /gene="ispF"
                     /locus_tag="VEC56_06300"
     CDS             1173..1691
                     /gene="ispF"
                     /locus_tag="VEC56_06300"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXV13799.1"
                     /translation="MTRTGIGYDVHAVAAGRKLMLGCVHFDDAKVGLAGHSDADVVAH
                     AICDAILGAAALGDIGEHFPNTEAEWKDAESKEFLIKVAGMVRELGYDIINVDCTVLS
                     DAVKLGDRKRDMVAAIAGYLDVAPNCVNVKATTFEGKGAIGRGEAIACEAIATLERGA
                     APKLSEWREDVL"
     gene            1692..3131
                     /gene="gltX"
                     /locus_tag="VEC56_06305"
     CDS             1692..3131
                     /gene="gltX"
                     /locus_tag="VEC56_06305"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXV13800.1"
                     /translation="MAKLPDVRVRFAPSPTGYLHVGGARTAIFNHLFARHNGGTFILR
                     IEDTDAERSTRESEVSLLEDLRWLGLDWDEGPDVGGPVGPYRQSERMAIYREVADKMV
                     AQGRAYPCFCTEAELELKREEAAAASMPPQYDGTCRNLTAEEIAAKRATGLPEAIRFR
                     VGAGVVRFHDAVRGDMELAHDMVGDWVTLRSNGLPTYNFAAAVDDHLMKISHVIRGEE
                     HLPNTLRQIMIYDALGATHPIFAHVPLILAEDRSKLSKRHGASSVGDLRASGYLPDAA
                     TNYLLLLGWSHPQSKEIMPREEMVKSFGIERIGKAAAVFDKKKLLWMNGQYIRKLDLD
                     TLIALVEPYTPEFLKQRYDAKTRREIVSILQDSIDTLADFAHRASIFEPNVAYDEETQ
                     ALLKTDSARRVIESMVKRMHAHEAPITPESFKEMILATGQETGLKGKDLYFPIRGAMT
                     GSVHGPDLTRVAAVKGKDVVLRLLEGVIR"
     gene            3128..>3793
                     /locus_tag="VEC56_06310"
     CDS             3128..>3793
                     /locus_tag="VEC56_06310"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HXV13801.1"
                     /translation="MKALVATILVLVAAAPCLAHDTWLMPREPQVTEGKQATVELTSG
                     MSFPQTESAIAADRVQKGAWRTGRAKGKLDKWEEGSSSLVMHLTAVGTGTMAMWLTLK
                     PKDIELTDEDVEHYFEEIGASESVRRDWESREPGTVFKERYTKHAKSFMRVGDGGEDP
                     TCLQPAGLAIELLPQSDPTALSVGDKLVVKAVRGGDDELEQFAVGIVCGATGASEILR
                     TNES"
BASE COUNT          706 a         1195 c         1332 g          560 t
ORIGIN      
        1 ttttggacga agtggaaacc acctcgcggc acctgcgcga gatcgcggtc tcgctctccg
       61 gcgacggacg cctgcaagag ggcgtcaacg acttcgcgga ggcgagcaag aacgtgcgcg
      121 ggatgacgga gcgcaacaag gaccgcctcg accgggcgct gatgcgattc gagcgctcgg
      181 ccacgatgct cgacagcctg atcacgacgc gttacccggc catcgactca tcgctcgcgg
      241 ccatcgggcg cgcgggaggc aaagcggaga cgacggtcga caacctggcc gaagtgtcga
      301 aggacctgcg cgagatcacc gccaagctgc gcgcgggcga aggctccgcg ggccggttgc
      361 tcaacgacga caccttcatt acgcggctcg agtcgacggt ggccgaactc gattcgctgg
      421 tggcggacat ccagcgaaac ccgggccgct acgtcaagtt cagtctgttt tagcgccatg
      481 aaggcggccg cactcatcat ggcgggcggg agcggcgagc gcttcgtcgc cgggagcgag
      541 cgcgaggcca agcagctggc gccgatcgcg ggccgcgcga tgatcgcgtg gtcggcggaa
      601 gcactcgcgc gctcgacgcg cgtcgttcga ttgatcgttg cgctgccggg gggcatggag
      661 gcaggtgtgc gggcggtgct gccgcccgat gcggtgcgaa agatgcacgc gttcgtcgaa
      721 ggcggagcga cccggcagga gtcggtgttc aacgcgctgc gcgcgctgcc ggacgacgtc
      781 acccacgtgc tgatccacga cgctgcgcga ccgtgcgtgt cgtcgcaatt gatcgagcgc
      841 gtgctcgacg cgctggaaac gcgcgacgcc gtcgtccccg cgaccgccgc gaccgacacg
      901 ctcgtgcgcg actatgaagg acgcgtcgac gcgattatcg atcgcgtgca cgtggcgggc
      961 gtgcagacgc cgcaggcgtt caggcgcgag ttgattctcc gcgcgcaccg cgaggcgagg
     1021 gcgcgcggat tccattcgag cgacgacggg tcgctcgtgc tggcgctggg agagacggtg
     1081 cacaccgtcc ccggcgagcg gaccaatatc aaggtgacgt ttcgtgacga cgtcgcaatc
     1141 gccgaagcga tcttgaagaa cggagtacga acatgacgcg aacgggaatc ggatacgacg
     1201 tgcacgcggt tgccgcgggg cggaagttga tgctggggtg tgtccacttc gacgacgcca
     1261 aggtgggcct cgcgggccac tcggacgcgg atgtggtcgc gcacgcgatc tgcgacgcga
     1321 tcctgggggc cgccgcgctg ggcgacatcg gcgagcactt ccccaacacg gaggcggagt
     1381 ggaaggacgc cgagagtaag gagttcttga tcaaggtggc ggggatggtg cgcgagctgg
     1441 gctacgatat tatcaatgtc gattgcaccg tcctctcgga cgcggtgaag ctgggcgacc
     1501 gcaagcgcga catggtggcc gccatcgcgg gctatctcga cgtcgcgccc aattgcgtca
     1561 acgtcaaggc gaccaccttc gagggcaagg gagccatcgg acgcggtgag gcaatcgcgt
     1621 gcgaagcgat cgcgacgctc gagcgcggcg ccgcgcccaa gttgagcgag tggcgcgagg
     1681 acgtgctcta gatggcgaag ctccccgacg tgagggttcg cttcgcgccc agcccaaccg
     1741 gctatctgca cgtcggcggc gcgcgcacgg caatcttcaa ccacctcttc gcgcgacaca
     1801 acggcggcac cttcatcctg cgcattgagg ataccgacgc ggagcggagc acgcgcgaga
     1861 gtgaggtctc gctgctcgaa gacctgcgct ggctcggcct cgactgggac gagggccccg
     1921 acgtgggcgg cccggtggga ccgtaccgcc agtcggagcg catggcgatc tatcgcgagg
     1981 tcgcggacaa gatggtcgcg cagggtcgcg cgtacccgtg tttttgcacc gaagccgaac
     2041 tcgagctcaa gcgcgaggaa gcggccgccg cgtcgatgcc cccgcagtac gacggaactt
     2101 gtcgcaacct gacggccgaa gagatcgcgg ccaagcgcgc aacgggatta ccggaagcga
     2161 ttcgctttcg cgtcggcgcg ggcgtggtgc gcttccacga cgccgttcgc ggcgacatgg
     2221 agctcgcgca cgacatggtg ggcgactggg tgacgctgcg ctcgaacggg cttccgacct
     2281 acaacttcgc ggccgcggtc gacgatcacc tgatgaaaat ctcgcacgtg atccgcggcg
     2341 aggagcacct gcccaacacg ctgcggcaga tcatgattta cgacgcgctg ggcgcgaccc
     2401 accccatctt cgcgcacgtc cccttgatcc tggccgaaga ccgctcgaag ctctcgaagc
     2461 gacacggcgc gagctcggtg ggcgacctgc gcgcgagcgg ctacctcccc gacgcggcga
     2521 ccaactacct cctgctgctc gggtggtcgc acccgcagag caaggagatc atgccgcgcg
     2581 aggagatggt gaagagtttc ggcatcgagc gcatcggcaa ggcggcggcc gtgttcgaca
     2641 agaagaagct cctgtggatg aacgggcagt acatccgcaa gctcgacctc gacacgctga
     2701 tcgcactggt cgagccgtac acgcccgagt tcttgaagca gcgctacgat gcaaagacgc
     2761 gccgcgagat cgtctcgatt ctgcaagaca gcatcgacac gctcgcggac ttcgcgcatc
     2821 gcgcgagcat cttcgagccc aacgtcgcct acgacgagga gacccaggcg ctcctcaaaa
     2881 cggactcggc acgccgcgtg atcgagagca tggtgaagcg gatgcacgcg cacgaagccc
     2941 cgatcacgcc cgaatcgttc aaggagatga tcctcgcgac cgggcaggaa accggactca
     3001 aaggcaagga tctgtatttc ccgattcgcg gcgcgatgac gggatcggtg cacggtcccg
     3061 atctcacgcg cgtggccgcg gtgaagggga aagacgtcgt tcttcgacta ctagaaggag
     3121 tgatccgatg aaagcactcg ttgcgacgat cctggtactg gtggcggccg ctccgtgtct
     3181 ggcccacgac acgtggctca tgccgcggga accgcaggtc acggaaggca agcaggcgac
     3241 ggtggagttg acgagcggga tgtcgtttcc gcaaacggag agcgcgatcg cggccgaccg
     3301 cgttcagaag ggcgcgtggc gcacgggccg cgccaagggc aagctcgaca agtgggagga
     3361 gggcagctcg tcgctcgtca tgcacctgac cgcggtcggc accggcacca tggcgatgtg
     3421 gctcaccctc aagccgaagg acatcgagct caccgacgag gacgtcgagc actacttcga
     3481 agagatcggg gcgtcggaat cggttcgccg cgactgggag agccgcgagc cggggacggt
     3541 cttcaaggag cggtatacca agcacgcgaa gtcgttcatg cgcgtcggcg acggcggcga
     3601 ggatccgacc tgcttgcagc cggcgggtct cgcgatcgag ctcctgcccc agagcgaccc
     3661 gaccgccctc agcgtcggcg acaagctcgt ggtcaaggcg gtccgaggcg gtgacgacga
     3721 gctcgagcaa ttcgcggtcg gcatcgtgtg cggtgccacc ggcgcgtcgg agatccttcg
     3781 caccaacgag tcg
//
LOCUS       DASPGK010000232         9351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_996185, whole genome shotgun sequence.
ACCESSION   DASPGK010000232 DASPGK010000000
VERSION     DASPGK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996185"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..475
                     /gene="sodC"
                     /locus_tag="VEC56_06315"
     CDS             8..475
                     /gene="sodC"
                     /locus_tag="VEC56_06315"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HXV13802.1"
                     /translation="MADEIVVTVHLATEAGPGKAIGTIRVEDTAHGALFTPSLRDLPP
                     GLHGFHVHEHAHCGPAEKEGKMMPGLAAGNHFDPGKTGKHLGPYGDGHLGDLPPLYVD
                     ADGAATLPVLAPRLKVADLKGRSLMIHAGGDNFSDQPAALGGGGARIACGTVE"
     gene            600..911
                     /gene="rplU"
                     /locus_tag="VEC56_06320"
     CDS             600..911
                     /gene="rplU"
                     /locus_tag="VEC56_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXV13803.1"
                     /translation="MYAILKISGQQFKATPNAILRVPKMEAEVGGNVTFSDVLVWNDG
                     QNTEIGAPLVAGKSVRGEIVKHARADKIIVFKKKRRKGYRRKNGHRQWYTEIRLTNFS
                     G"
     gene            928..1185
                     /gene="rpmA"
                     /locus_tag="VEC56_06325"
     CDS             928..1185
                     /gene="rpmA"
                     /locus_tag="VEC56_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXV13804.1"
                     /translation="MAHKKGVGSSKNGRDSHGQRLGVKRFSGEFITAGSIIVRQRGTR
                     IHPGRNVGRGKDDTLFAKVTGTVRFESFGKNRRRVSVVEAN"
     gene            1229..1984
                     /gene="kdsB"
                     /locus_tag="VEC56_06330"
     CDS             1229..1984
                     /gene="kdsB"
                     /locus_tag="VEC56_06330"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXV13805.1"
                     /translation="MSFAIVLPARFGSTRFPGKPLATILGKPLIEWVYQGASRVRGAS
                     RVLIATDDARIATAVRLFGGEVVMTRNAHQTGTDRVAEVAKTLRTDVVVNLQGDEPIF
                     DPGMVEEMVAVLARDPEVDIATACHPIQDTESLQSPHVVKVVIDQLGRAMYFSRAPIP
                     SGALFDGAKAYRHVGVYAFRREALARFAKLPRTPLEIAERLEQLRALEHGMKIAVVET
                     RHATVGVDVPADIKTVERFVEESLDSTHGSRTT"
     gene            2013..3644
                     /locus_tag="VEC56_06335"
     CDS             2013..3644
                     /locus_tag="VEC56_06335"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXV13806.1"
                     /translation="MKYIFITGGVVSSLGKGIAAASIGLLLKQRGLRVALQKFDPYLN
                     VDPGTMSPFQHGEVFVTDDGGETDLDLGHYERFLDQNLTREHSVTAGKVYQAVIEKER
                     AGGYLGKTVQVVPHITDEIKTRIKAVGTSGDIDVIITEVGGTTGDIEGLPFLEACRQM
                     RHEEGADNCLFVHLVLVPYIKAAKEIKTKPTQHSVKGLLEIGIQPDILLCRTEVPLGP
                     EIRAKIALFCNVREPAVIEARDVASIYHVPLHFHREGLDDIIVKLLHIDAREPELDEW
                     ERMIQRMTAIEQSIRIAVVGKYVQIQDAYKSIIEAFTHAGVQNDVKVEVQWVDAEDVE
                     KLGAEEALRGAAGILVPGGFGERGIAGKVDAIRYARENEIPFLGICLGLQCAVIEFAR
                     DVCGIKDALSSEFSPNCPNPVIDLMEGQTHSGKKGGTMRLGAYPCKIKPGTLAEDLYG
                     ATEVSERHRHRWEVNNEYRDVLERNGLVFSGTSPDGSLVEIIELSEHPYFIASQFHPE
                     LKSRPHRPHPLFFGLVRAAKSQRNSHSTSARRVSA"
     gene            3664..4491
                     /gene="kdsA"
                     /locus_tag="VEC56_06340"
     CDS             3664..4491
                     /gene="kdsA"
                     /locus_tag="VEC56_06340"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXV13807.1"
                     /translation="MAVSASIGSIRVGDGEKPLLIAGPCVIESEELTLSIAESIAKLA
                     ETRAFHFVFKASYLKDNRSAGTSFRGPGLEEGLRVLEKVRARVGCPVLSDVHDVSEVA
                     AAAATLDILQVPAFLSRQTSLIEAVGKTGKAINIKKGQFLAPEDMGNVVEKARAAGAR
                     DVLLTERGTSFGYHDLVVDFRGFARMRATGCPVIFDVTHSLQRPGGLGTASGGEPALA
                     AMMARAAAAVPVDGFFIETHPDPARALSDAASMLPLDRLPALLRDVARVASAARDTR"
     gene            4683..6950
                     /locus_tag="VEC56_06345"
     CDS             4683..6950
                     /locus_tag="VEC56_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXV13808.1"
                     /translation="MDRTVLPIAEPPIPVITEKDARKATPPPRFEVKAPENAPNVVIV
                     LIDDFGFGHSSTFGGPVQTPTLERLASQGLRYNRFHTTALCSPTRTALLTGRNHHINN
                     AGAIMELATAFPGNTGVRPNSVTPLAEILRLNGYSTAAFGKYHETAPWEASVSGPFDR
                     WPTRSGFDKFYGFIGGETNQWAPAVYDGVTRVEPPHDPNYHFTTDMTNQAVSWVRYQQ
                     ALTPDKPFYIYFATGATHAPHHVGKEWIAKYRGKFDQGWDKLREETLARQLQLGVVPT
                     GTQLTARPAEIPAWDSLTDDQKTLFRRQMETFAGFGEHTDHEVGRLVQALEDMGELDN
                     TLFFYIAGDNGSSAEGGPDGAYNEILALNGIIADIAANLKHLDNWGDASTFPHFSVGW
                     AHAGNTPFQWTKQVASHFGGTRNGMVIHWPARIKNAHGEVRSQFHHVIDIAPTVLEAA
                     GLPEPKSVDGTVQYPMDGVSMVYTFDDPAAPDRRTTQYFEMFGNRAIYHNGWVAATRH
                     STPWVMVPQLPPFDEDVWELYNVSEDFSQANDLAAQNPEKLKELQAVFDQEAIKNHVY
                     PLDDRRSERFDARIAGRPDLMGPRTSLTLYEGMTGITENAFINIKGRSSAITAEVDVP
                     ANASGVIISQAGRFGGWSLYMKNGRVSYAYNFGGLQIWTVSSPVALTPGKHTVLQEFV
                     YDGGGPGKGATVKLTIDGRPAGETRVERTMPFIYSADEGVDVGTDNETTVTNDYKAGS
                     NKFTGRIVKVTVEQK"
     gene            6985..8184
                     /locus_tag="VEC56_06350"
     CDS             6985..8184
                     /locus_tag="VEC56_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13809.1"
                     /translation="MAVNAEERRISDLTDFSITDCGAMDRLMVRLGISRPPDARYLVR
                     RAIAAALIAWLPLLALASFAGHEGARPAISFLHDIAVHVRFILVVPLLVLAEAAIGQR
                     TRLVAAGFLTSGLVTEPDAPRFQSAVRSTRKLVDSIWVEIVLLVLTYVLVWFAIQSVK
                     SDGVLVWFERAAPGGHRLTLAGWWYALVSVPLASFLFLRWLWRYLVWTWFLHRLSRLR
                     LRLVASHPDRAGGLGFINVGHAAFAVIAVAAGAVVSAAAANRILYEGASLRTYQSAII
                     GFIVIAVVVGIAPLLSFMRPLILTKRRGLVEYGDLATRYVQSFERKWVSGGGDSKEPL
                     LGTGDIQSLADLGGSFERVDHMKVMPFDRRTVMAFAFSAAAPMLPLLLTVMPLSEMIR
                     FLLKAMI"
     gene            8248..9252
                     /locus_tag="VEC56_06355"
     CDS             8248..9252
                     /locus_tag="VEC56_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXV13810.1"
                     /translation="MKIERNTEADALVAGARRVIDEEIAGLKTLRDRLGPELAQAVDA
                     IVACRGRLIVSGVGKSGQIAKKIASTLTSTGTPSFYVHAFEASHGDLGLVYPDDVVVI
                     ISKSGMGDELRDFVPALKKLGVTIIAMTASRSSYLAQHSDIVLEFDAAHEAGCLGLAP
                     TTSATVSLVLGHVLASALMERKGVTAEEFARTHPGGMLGKRLTVTVANVMRTGNAVPV
                     VQESTTLREALFEIMEKSIGCTGVVDARGKLTGIITDGDLKRILAHRDDALELPVVEV
                     MTHSPKTIDSTTLAADALAKMELNMPGPLLMYFIVDPEQRPVGLLHLHDILRAGLRAE
                     "
BASE COUNT         1717 a         3060 c         3014 g         1560 t
ORIGIN      
        1 ggccgccatc gccgacgaaa tcgtcgtcac cgtccatctc gccaccgaag ccgggcccgg
       61 caaagcaatc ggcaccatca gggtggagga caccgcacac ggcgcgctct tcacgccgtc
      121 gctgcgcgat ctcccgccgg gcctgcacgg cttccacgtg cacgaacacg cgcactgcgg
      181 cccggccgaa aaagagggca agatgatgcc cggtctcgcg gccggcaacc acttcgaccc
      241 cgggaagacg ggtaagcacc tgggtcccta cggcgacggg cacctcgggg acctgccgcc
      301 gctctacgtc gacgcggatg gggctgcgac cctcccggta ctcgcgcccc ggctcaaggt
      361 cgccgacctc aaggggcggt ccctcatgat ccacgccggc ggggacaatt tctccgacca
      421 gcccgccgcc ctgggtgggg gcggggcccg aatcgcgtgc gggacggtgg aataggctcc
      481 ggggcgtacg gggcggccaa aaaccggccc ttgacacttt tttctcgatg cattacaaat
      541 gtacggttgc ctcgggcggc caaaggtgcg cccgggctgg aacccgaagt ggagcaatca
      601 tgtacgcgat cctgaaaatc agcggccagc agttcaaggc caccccgaac gcgatcctcc
      661 gcgtccccaa aatggaagcc gaggtcgggg gcaacgtcac cttcagcgac gtcctcgttt
      721 ggaacgacgg ccagaacacc gagatcgggg caccgctggt cgcgggcaag tcggtgcgcg
      781 gcgaaatcgt caagcacgcg cgcgcggaca agatcatcgt cttcaagaag aagcgccgga
      841 agggttaccg tcgcaagaac ggccaccggc agtggtacac cgagattcgc ctcaccaatt
      901 tcagcgggtg agggtggacg taacagcatg gctcacaaaa aaggtgtagg cagctccaag
      961 aacggtcgcg acagccacgg tcagcgactc ggcgtaaagc gtttctccgg cgagttcatc
     1021 accgccggct cgatcatcgt gcgccagcgc ggcacacgaa ttcaccccgg ccgcaacgtc
     1081 ggccgcggaa aggacgatac gctctttgcg aaggtcaccg gcacggtcag gttcgagagt
     1141 ttcggtaaga accgccgccg cgtcagcgtt gtcgaagcga actagcgctt cgaagaaacg
     1201 acccaccggc cgaacggccc ccgcatctct gtccttcgcc atcgtgctcc cggcccgctt
     1261 cgggtcgacg cgctttcccg gcaagcctct cgcgaccatc ctcggtaagc ccctgatcga
     1321 atgggtctac cagggggctt cgcgcgtgcg cggggcttcg cgtgtgctca tcgccaccga
     1381 cgacgcgcgc atcgcgaccg ccgtgcgcct gttcggcggc gaagtggtaa tgacgcgcaa
     1441 cgcccaccag accggcaccg accgcgtggc cgaagtggcg aagacgctgc gcaccgacgt
     1501 ggtcgtcaat ctgcagggcg acgagcccat cttcgacccc ggcatggtgg aagagatggt
     1561 cgcggtcctc gcgcgcgacc ccgaggtcga catcgccacc gcctgccatc ccattcaaga
     1621 caccgagtct ctccaaagcc cgcacgtggt caaggtggtc atcgaccagc tcggccgcgc
     1681 gatgtacttc tcgcgtgcac ccattcccag cggggctctc ttcgacggcg ccaaggccta
     1741 tcgccacgtg ggcgtgtacg cgttccgccg cgaggcgctg gccagattcg cgaagctccc
     1801 cagaactcca ttggagatcg cggagcgcct ggagcaattg cgcgcgctgg agcacggcat
     1861 gaagatcgcg gtggtggaga cgcgccacgc caccgtcggc gtcgacgtac cggccgacat
     1921 aaaaaccgtt gaaagattcg tcgaagagtc cctagactcg acgcacgggt cgcgaacgac
     1981 ataatgagca accagcgagc gaggtcggac gcatgaaata cattttcatt accggcggtg
     2041 tggtctcgtc cttgggcaag ggaatcgccg cggcgtcgat cggcctcttg ttgaagcagc
     2101 gcggactgcg cgtcgccctc cagaagttcg acccgtatct caacgtcgac cccgggacca
     2161 tgagtccctt ccagcacgga gaagtcttcg tcaccgacga cggcggcgag acggatctgg
     2221 acctcgggca ctacgagcgc ttcctcgacc agaatctcac ccgcgagcac agcgtcaccg
     2281 cggggaaagt ctaccaagcc gtcatagaaa aagagcgcgc gggcggctac ctgggtaaga
     2341 ccgtgcaggt ggtcccgcac atcaccgacg agatcaagac gcgcatcaag gcggtcggca
     2401 ccagcggcga catcgacgtc attattaccg aggtgggcgg caccaccggc gacatcgagg
     2461 gcctcccgtt tttggaagcg tgccgacaga tgcgccacga agagggcgcc gacaactgcc
     2521 tcttcgttca tttggtgttg gtgccctaca tcaaggccgc caaggagatc aagaccaagc
     2581 ccacccagca cagcgtcaag ggcctgctcg agatcggaat ccagcccgac atcctattgt
     2641 gccgaaccga agtgccgctg ggtcccgaga ttcgcgccaa gatcgcgctc ttttgtaacg
     2701 tgcgcgagcc ggccgtgatc gaagcgcgcg atgtcgcgtc gatctaccac gtgccgctcc
     2761 atttccaccg cgaaggcctc gacgacatca tcgtgaagct gctccacatc gacgcccgcg
     2821 agcccgagct cgacgagtgg gagcgcatga tccagcgcat gaccgcgatc gagcagagca
     2881 ttcgcatcgc ggtggtcggc aagtacgtgc agattcagga cgcctacaag tccatcatcg
     2941 aggcgttcac gcacgcgggc gtccagaacg acgtcaaagt cgaagtccag tgggtcgacg
     3001 ccgaagacgt cgagaagctg ggcgccgaag aggcgctgcg cggtgcggcc ggcatcctgg
     3061 tgccgggcgg cttcggcgag cgcggtatcg cgggcaaggt ggacgcgatt cgctacgcgc
     3121 gcgagaacga gattccgttc ctcggtattt gtttgggcct gcagtgcgcg gtgatcgaat
     3181 tcgcgcgcga cgtgtgcggc atcaaggacg ccctctccag cgagttcagc cccaactgcc
     3241 ccaatcccgt catcgacttg atggaggggc agacccactc cggcaagaaa ggcggcacca
     3301 tgcgcctggg cgcgtacccg tgcaagatca agccgggcac gctggccgaa gacctctacg
     3361 gtgccaccga agtgagcgag cgccaccgcc accgctggga agtcaacaac gagtaccgcg
     3421 acgtcctcga acgcaacgga ctcgtgttct ccggcacctc tcccgacggc agcctggtcg
     3481 agatcatcga gctatccgaa catccctact tcatcgcctc ccagttccat cccgaactca
     3541 agagccgacc ccatcgaccg caccccttgt tcttcggcct cgtgcgcgcg gccaagtccc
     3601 agcgcaactc gcactccaca tcggcccgcc gcgtgagcgc gtaggcgtcg tctcgagcgc
     3661 atcgtggcgg tctccgcgtc catcgggtcg attcgcgtcg gagacggcga gaagccgctc
     3721 ttgatcgccg gtccgtgcgt catcgagtcg gaggagttga cgctctcgat cgcggagtcg
     3781 atcgccaagc tggcggagac gcgcgcgttc cacttcgtct tcaaggccag ctatctcaag
     3841 gacaaccgca gcgcggggac gagctttcgc ggccctggac tcgaagaggg gctgcgcgtg
     3901 ctggagaagg tgcgcgcgcg cgtggggtgc ccggtgctct cggacgtgca cgacgtgagt
     3961 gaagtcgccg cggcagcggc gacactggac atcctccaag taccggcctt cctctcccgt
     4021 cagaccagcc tcatagaggc ggtggggaaa acagggaagg cgatcaacat caagaagggg
     4081 caattcctcg ccccggaaga catggggaac gtcgtggaga aggcgcgcgc cgccggcgcg
     4141 cgcgacgtgc tcttaaccga gcgggggacg tcgttcggct atcacgacct ggtggtggac
     4201 tttcgcggct tcgcgcgcat gcgcgcgacg ggctgcccgg tgatcttcga tgtcacgcac
     4261 tcgctgcaac gacccggcgg gctcggtacc gcttcgggcg gcgagcccgc gctggcggcg
     4321 atgatggcgc gcgccgccgc cgcggtcccg gtcgacggct tcttcatcga aacccatccc
     4381 gatcccgcgc gcgctctctc cgacgccgcg tccatgctcc cgctcgatcg tttgcccgcg
     4441 ctcttgcgcg acgttgcccg cgtcgcctcc gcggcccgcg atacccgcta gaacgtgcgc
     4501 ggttgcgcac aacacacgcc ctcgtgtact ctttcgcgac cccaattaag gagaattcca
     4561 tgtgccgcaa tcgattctca agcatcgtag ttatggctgc gttcttcgcg ctgccggtct
     4621 ttctcaactc gtgcgcgaag aaggcggatg aagcaaccgc gcccgccggt gcggcgtaca
     4681 cgatggaccg cacggttcta ccgatcgcgg agccgccgat tccggtcatc accgagaagg
     4741 acgcgcgcaa ggcgacaccg ccgccgcgct tcgaggtgaa ggcgcccgag aacgcaccca
     4801 acgtcgtgat cgtgttgatc gacgacttcg gcttcgggca ttcgtcgacc ttcggcggcc
     4861 cggttcagac gcccacgctg gaacgactgg cgtcgcaggg cctgcggtac aatcgattcc
     4921 ataccacggc actctgttcc cccacgcgca ccgcactgct gacggggcgc aatcatcaca
     4981 tcaataatgc cggcgccatc atggagctgg ccaccgcgtt tcccggcaac accggtgtgc
     5041 ggcccaacag cgtgaccccg ctggcggaaa ttctgcgctt gaacggctat agcaccgcgg
     5101 ccttcggcaa gtatcacgag accgcgccct gggaggcgtc ggtgtcgggc ccgttcgatc
     5161 gttggccgac gcgctcgggc ttcgacaaat tctatggctt catcggcggc gagaccaacc
     5221 agtgggcgcc ggccgtttac gacggcgtga cgcgcgtcga gccgccgcac gatccgaatt
     5281 atcacttcac gaccgacatg accaaccagg ccgtatcgtg ggtgcgttac cagcaagcgc
     5341 tgacacccga caagccgttc tacatctact tcgcgaccgg cgccacgcac gccccgcacc
     5401 atgtgggcaa agagtggatc gcgaagtacc ggggcaagtt cgaccagggc tgggacaagc
     5461 tgcgcgagga gacgctcgcg cggcagttgc agctgggggt ggtgccgacg ggcacccaac
     5521 tcacggcccg tccggcggaa atccccgcgt gggattcgct gaccgacgat cagaagacgc
     5581 tgttccggcg acagatggag accttcgcgg ggttcggcga gcataccgac cacgaagtcg
     5641 gccgcctggt gcaggcgctc gaggacatgg gcgagctcga taacaccctt ttcttctaca
     5701 tcgccggcga caacgggtcg agtgcggaag gcggtccgga cggggcgtac aacgagatac
     5761 tcgccttgaa cggcatcatc gccgacatcg ccgcgaattt gaagcacctc gacaactggg
     5821 gcgacgcgtc gacgttcccg cacttctcgg tgggttgggc gcacgcgggt aacacgccgt
     5881 tccagtggac caagcaggtc gcgtcgcatt tcggcggcac gcgcaatggc atggtgattc
     5941 actggccggc gcgcatcaag aatgcgcacg gcgaagtgcg ttcgcagttc caccacgtga
     6001 tcgatatcgc gccgacggtc ctggaagcgg ccgggttgcc ggaaccgaag tcggtagacg
     6061 gcacggtgca gtacccgatg gacggggttt cgatggtgta caccttcgac gatccggcgg
     6121 cacccgatcg ccgcacgacc cagtacttcg agatgttcgg gaaccgcgcc atctatcaca
     6181 atggttgggt ggcggcgacg cggcactcga cgccatgggt gatggtaccg cagttgccgc
     6241 cgttcgacga ggacgtgtgg gagttgtaca acgtgagcga agacttcagc caggccaacg
     6301 acctggcggc ccagaatccg gagaagctca aggagctgca ggccgtcttc gaccaggaag
     6361 cgatcaagaa ccacgtctat ccgctcgacg accggcgctc cgagcgcttc gacgcacgca
     6421 tcgccggacg cccggatctg atggggccac gcacgtcgtt gacgctgtac gaaggcatga
     6481 ccggtattac cgagaacgcg tttatcaaca ttaaggggcg ctcgtcggcc atcacggcgg
     6541 aggtcgacgt gcccgcgaac gccagcggcg tcatcatctc gcaggcgggc cgcttcggcg
     6601 gctggagtct gtatatgaag aacggccgcg tgagctacgc gtacaatttc ggcggcctgc
     6661 agatctggac cgtctcgtcg ccggtcgcgt tgacgccggg gaaacacacg gtattgcagg
     6721 aattcgtgta tgacggcggc ggccccggca agggcgcgac ggtcaagctc accatcgacg
     6781 gcaggcccgc gggagagacg cgcgtcgagc gcaccatgcc cttcatctat tcggccgacg
     6841 agggcgtcga cgttggaacc gacaacgaga cgaccgttac caacgactac aaggcgggga
     6901 gcaacaagtt cacgggacga atcgtcaagg tgacggtcga gcagaaatag ccgcatcgca
     6961 cgtctgcgcg aagaggacta tctcatggcg gtgaacgcgg aagaaagacg catctcggat
     7021 ctgaccgatt tctcgatcac cgactgcggt gcaatggatc ggctgatggt gcggctggga
     7081 atcagccggc cgcccgatgc gcgctacttg gtgcgacgcg cgatcgcagc ggcgctcatc
     7141 gcgtggctgc cgctgctcgc gctcgcctcg ttcgcgggcc acgagggcgc acgccccgcc
     7201 atttcgttct tgcacgacat cgcggtgcac gtccgcttca tcttggtggt cccgctgctg
     7261 gtattggccg aggcagccat tggtcagcgc acgcgtctgg tggccgcggg attcctgacc
     7321 tcggggctgg tgacggagcc agacgcgccg cgttttcagt ccgcggtgcg ctccacgagg
     7381 aagctcgtcg actccatctg ggttgaaatt gtcctgctcg tgttgaccta tgtgttggtg
     7441 tggtttgcga tccagagcgt gaagagcgac ggcgtcctgg tctggttcga aagagcggcg
     7501 ccaggtggtc atcggctcac gctggcaggt tggtggtatg ccctggtctc ggtaccgctc
     7561 gcgagcttcc tgttcctgcg ttggctgtgg cgttacctgg tgtggacgtg gttcctgcat
     7621 cgcctctcgc gcttgagact gcggctggtg gccagccacc cggatcgtgc gggggggctc
     7681 ggctttatca acgtcggtca cgccgcgttc gcggtgatcg cggttgcggc tggcgcggtg
     7741 gtgtccgcgg cggcggccaa tcgcattctc tacgaaggcg cctcgctgcg gacctaccaa
     7801 tccgcgatca tcgggttcat cgtgatcgcg gtggtggtcg ggatcgcgcc gttgttgtcg
     7861 ttcatgcgcc cgctcattct caccaagcga agggggctgg tcgagtacgg cgatctcgcg
     7921 acccgctacg tgcaatcctt cgagcggaaa tgggtgtccg gcggcggcga ctcgaaggag
     7981 ccgctgttgg gtacgggcga tattcagtcg ctggccgacc ttggcgggag cttcgaacgc
     8041 gtggatcaca tgaaggtcat gccgttcgac cggcgcacgg tcatggcttt cgcgttctcg
     8101 gcggcggcac cgatgcttcc gctcctgctc acggttatgc cgttgagcga gatgatccgg
     8161 ttccttctca aggcgatgat ctagggggct gcggggcgtc gccatgaggc atacccgagt
     8221 actctcgaag gtggtaatct cttggccgtg aagatcgaaa gaaacaccga ggccgacgcg
     8281 ctcgtcgcgg gcgcgcgccg tgtgatcgac gaggagatcg cggggctcaa gaccttgcgc
     8341 gaccgcctgg gtcccgaact ggcccaggcc gtcgatgcca tcgtcgcgtg ccgcggccgc
     8401 ttgatcgtct cgggtgtggg gaagtccggc cagatcgcga agaagatcgc gagcacgctc
     8461 acctcgacgg ggacgccgag cttctacgtc cacgccttcg aagcctccca cggcgacctg
     8521 gggctggtgt atcccgacga tgtcgtcgtc atcatatcga agagcgggat gggcgacgag
     8581 ctgcgcgact tcgtccccgc cctgaagaag ctcggagtca cgatcatcgc gatgaccgcc
     8641 tcccgcagct cgtacctcgc gcagcacagc gacatcgtgc tcgagttcga cgccgcgcac
     8701 gaagccggct gcctgggctt ggcacccacc accagcgcca ccgtgtcgct cgtgctcggc
     8761 cacgtgctcg cgagcgccct catggagcgc aagggtgtca ccgccgaaga gttcgcgcgc
     8821 acgcaccccg gcggaatgct gggcaaacga ctcaccgtga cggtcgcgaa cgtgatgcgc
     8881 accggcaacg ccgtgccggt ggtgcaggaa agcaccaccc tgcgcgaagc cctattcgag
     8941 atcatggaaa agagcatcgg ttgcaccggt gtggtcgacg cgcggggaaa gctcaccggc
     9001 atcatcaccg acggcgactt gaagcgcatc ctggcccacc gcgacgacgc gctcgagctt
     9061 cccgtggtcg aagtaatgac gcacagcccc aagaccatcg actccacgac gctcgccgcg
     9121 gacgcgctgg cgaagatgga gctcaacatg ccgggtccgc tcctcatgta cttcatcgtc
     9181 gatcctgagc aacgaccggt cggcctcctc cacctccacg acatcctgcg cgccggactc
     9241 cgcgcggagt agtacgcgca cgacagtgcg cgaaatagag gaagagtcgc atgagacttc
     9301 gaatcttgtt cccgttggta ccggcgatcg tccttggcct cgcggcgcgc g
//
LOCUS       DASPGK010000233         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1114670, whole genome shotgun sequence.
ACCESSION   DASPGK010000233 DASPGK010000000
VERSION     DASPGK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114670"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VEC56_06360"
     CDS             <1..240
                     /locus_tag="VEC56_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HXV13811.1"
                     /translation="IPVTTLVADTLSVALVEGWRGEVCHVALTDESGRLRRYKIVDPS
                     FHNWSALATAMRTQAISDFPLCNKSFNLSYCGFDL"
     gene            243..1043
                     /locus_tag="VEC56_06365"
     CDS             243..1043
                     /locus_tag="VEC56_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV13812.1"
                     /translation="MSILETIKYRLRHGCATMDYPDGPAPPLPDRHGGALRVEPSKCA
                     DGCTACESVCPTLAITRSPGRPVALDLGRCLFCSACVEACPTHAITHTGDHRLAATRR
                     EDLVLGEGGEEVRLACALDEKLRALFGRSLRLRQVSAGGCNGCESDINVLGTIGWDLG
                     RFGIQFVASPRHADGLLVTGPVTRNMRVALEKTYAAVPAPKIVIAAGACAIAGGPFVD
                     LPQVGNGAANVVPVDLYIAGCPPHPLTVLDGLLRLIGRLEDQQRTPQR"
     gene            1071..2174
                     /gene="mqnC"
                     /locus_tag="VEC56_06370"
     CDS             1071..2174
                     /gene="mqnC"
                     /locus_tag="VEC56_06370"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HXV13813.1"
                     /translation="MLNTIRAKIENDERITRDEGLFLLREAPLLELAPLAMAARWRLN
                     PERRVTFVIDTNLNYTNTCDAFCAFCAFYRTPGQQGEYTYSIEQMMEQFARAREMGVT
                     TVLLQGGLNKAIPFQYYLDLVRETRTRFPDIHAHFYSAPEVMKMTEVSGLSLDQVIAR
                     LYDAGLRSIPGGGAEILTDRVKEVISKLWPKGTTKDWVDVHRAAHRGGMRSTATMMYG
                     HVETDEDVIEHLEVVRALQDETGGFTAFIPWSYKRDQNPLARRVISEAGPNRYLRIIA
                     VSRIYLDNVQHVQASWFSEGKKTGVVALHFGGDDFGGTIFDENVMQEAGFFNRTTIDE
                     IVALIRDAGFAPAQRTTLYEIVREFPAVEAAAL"
     gene            2271..2717
                     /locus_tag="VEC56_06375"
     CDS             2271..2717
                     /locus_tag="VEC56_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXV13814.1"
                     /translation="MFGKLASSEGVAKDFGLLLIRLGVSVFMFCLHGYGKITGGPERW
                     EGTGGAMANLGITFMPVAWGFLAAFAESVGSILIVLGPLFRVATLMLAFNMFVATMMH
                     MNLPTDNPASGFAGAEKALVYMTVYLGLFFAGPGRFAAKLTKAERR"
     gene            2784..3167
                     /locus_tag="VEC56_06380"
     CDS             2784..3167
                     /locus_tag="VEC56_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13815.1"
                     /translation="MPVATQIDPEHKLVTFECSGTVVFDDVRSAFQGMFEDPRFTPGI
                     NALWDLRNASIGVRMQELPGLLDMISSRQSDRGKGYRVALLVAGSPDFGLSTLFEMSA
                     HAMPFAVRVFRSRTEATRWLAGQDD"
     gene            3172..3591
                     /locus_tag="VEC56_06385"
     CDS             3172..3591
                     /locus_tag="VEC56_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13816.1"
                     /translation="MTRWFRAGAIGLLVVAVLHTFGHFGGGPKDPARIQIEEAMNAYR
                     FDIMGMHPSASDITNSLSLMMTIMLVFAGVLDLIVVGALSGNGRVLRRLAWVNAVGVG
                     ALAALFAYYRIPPPMATLAVVCLFFVVAALRPAARRF"
     gene            3637..>4505
                     /locus_tag="VEC56_06390"
     CDS             3637..>4505
                     /locus_tag="VEC56_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HXV13817.1"
                     /translation="MKSQVGVSDKPTISTKAPVSAFLRHHFRHFNAAALIDAADAYRR
                     HLDGGGKMMVTVAGAMSTAELGISLAEMIRRDKVHIISCTGANLEEDVYNLIAHDFYE
                     RIPHYRDLTPHDEKRLLDRHLNRVTDTCIPEEEAMRRLEAVVLEEWSRADAKGERYFP
                     HEFIFKAIRSGALKQHYQIDPKDSWIVAAAEKNLPMVVPGWEDSTLGNMFAASLIRGD
                     VKKATTMRSGVEYMVDLANWYQSTAATSSIGFFQIGGGIAGDFPICVVPMLEQDLGER
                     TPLWGYFCQISDST"
BASE COUNT          761 a         1474 c         1448 g          822 t
ORIGIN      
        1 attccggtga ccacgctggt cgccgacacg ctgtcggtag cactcgtcga aggctggcgc
       61 ggcgaagttt gccacgttgc actcaccgac gagtccggtc ggttgcgtcg ctacaagatc
      121 gtggacccgt ccttccacaa ctggtccgcg ctcgccaccg ccatgcgcac gcaagcgatt
      181 tcggattttc cgttgtgcaa caagagcttc aacctttcat actgcgggtt cgatctctga
      241 ccatgtctat tctggaaacc atcaaatatc gactgcggca cgggtgcgcg accatggact
      301 atcccgacgg cccggcgccg cccttgcccg atcgccacgg tggcgcactg cgcgtggagc
      361 cgtcgaaatg cgcggacggc tgcacggcgt gcgaatccgt ctgcccgacg ctcgccatca
      421 cgcgctcgcc cggtcggccg gtcgcactcg acttgggtcg atgtctcttt tgctccgcgt
      481 gcgtggaggc ttgcccgacg cacgccatca cgcacaccgg agaccaccgc ctcgcggcga
      541 cgcgccgtga agacttggta ctcggtgagg gcggggagga agtgcgcctc gcgtgcgctc
      601 tggacgagaa gctgcgcgcg ttgttcgggc ggtctttgcg cctccggcaa gtcagcgcgg
      661 gtgggtgcaa cgggtgcgag agcgacatca acgtgctcgg caccatcggg tgggacttgg
      721 gccgcttcgg catccagttc gtggcctccc cccgccacgc cgacggcctc ttggtcacgg
      781 gcccggtgac gcgcaacatg cgcgtcgcgt tggagaagac ctacgccgcg gtgccggccc
      841 cgaaaatcgt catcgccgcg ggcgcctgcg ccatcgcggg cggtccgttc gtcgatctgc
      901 cgcaggtcgg taacggggcc gcgaatgtcg tccccgtcga tctctacatc gccggctgcc
      961 cgccccaccc gttgacggtt ctcgacggtc tactccgact catcgggcgg ttggaagatc
     1021 agcagcgcac accgcagcgg tgattcgcac attgtgctag actggcggcc atgctgaaca
     1081 cgatccgcgc gaagatcgaa aacgatgagc gcatcacgcg cgacgagggt ttgttcctgc
     1141 tccgcgaggc tccgctgctg gagctcgcac ccctcgcgat ggcggcgcgc tggcggttga
     1201 acccggagcg ccgcgtcacc ttcgtcatcg acaccaatct gaattacacg aacacgtgcg
     1261 acgccttctg cgccttctgc gccttctatc gcacgcccgg acagcagggc gagtacacct
     1321 attccatcga gcagatgatg gagcagttcg cccgcgcgcg cgaaatgggc gtcaccaccg
     1381 tgctccttca gggtgggctc aacaaggcga ttccgtttca atattatctc gacctggtaa
     1441 gagagacgcg cacgcgtttc ccggacatcc acgcccactt ctactccgcg cccgaggtca
     1501 tgaagatgac cgaggtgtcg gggctatcgc tcgatcaggt catcgcgcgc ctctacgacg
     1561 cgggcctgcg ctcgattcca ggcggcggcg cggagattct caccgaccgc gtgaaagaag
     1621 tgatctcgaa gctgtggccc aagggaacca cgaaggactg ggtcgacgtc caccgcgccg
     1681 cgcacagggg cggcatgcgc tcgacggcca ccatgatgta cggccacgtc gagaccgacg
     1741 aagacgtgat cgagcacctc gaggtcgtgc gcgcgctcca ggacgaaacg ggcggattca
     1801 ccgccttcat tccctggagc tacaagcgcg accagaatcc tctcgcacgc cgcgtgatca
     1861 gcgaagcggg gcccaaccgc tacctgcgca tcatcgcggt ctcgcgcatt tatctcgata
     1921 acgtccagca cgtgcaggcg tcgtggttct cggaaggaaa gaagaccggg gtggtggcgc
     1981 tgcacttcgg cggcgacgac ttcggcggca ccatcttcga cgagaacgtg atgcaggaag
     2041 cgggcttctt caatcgcacc accatcgatg agatcgtcgc attgatccgc gacgccggct
     2101 tcgcgcccgc gcaacgcacc acactctacg agatcgtgcg ggagtttccg gccgtggaag
     2161 ccgcggcgct ttagcacctt ctggttcctg gctcgattcc tgccatcaaa gttggattgc
     2221 tccaactcgc ggtccgacgt atgctccccg gcgaaccaga ggaggtcccc atgttcggaa
     2281 aattggcttc gagcgaaggc gtggcaaagg acttcggatt gctgttgatc cggctcggcg
     2341 tcagcgtctt catgttctgt ctccacggct acggcaagat cacgggcgga cccgagcgct
     2401 gggagggtac cggcggggcg atggcgaatc tcggcatcac cttcatgccg gtcgcgtggg
     2461 gattcctggc cgcgttcgcg gaatcggtcg ggtcgatcct gatcgtgttg ggcccgctgt
     2521 ttcgcgtcgc cacgctcatg ctcgcgttca acatgttcgt ggcgaccatg atgcacatga
     2581 atctcccgac cgacaacccg gcgtccggtt ttgcgggggc ggaaaaggcg cttgtttaca
     2641 tgaccgtcta cctgggcttg ttcttcgcgg gacccgggcg cttcgccgcc aagctgacca
     2701 aggcggagag acgctagcgg cgcggggctg gcgaggcgcg ggcgtttgcg ctaccatcgt
     2761 gtccccggca cgtggggagg aacatgcccg tcgcgacaca gatcgatccg gaacacaagc
     2821 tcgtcacctt cgagtgcagc ggaaccgtcg tcttcgacga cgtccgctcc gcgtttcagg
     2881 ggatgttcga ggacccgcgc ttcacgcccg gcatcaacgc gctgtgggac cttcgcaacg
     2941 cgtccatcgg cgttcgcatg caagagctcc ccggcctgct cgacatgatc agctcgcgcc
     3001 agagcgaccg cggcaagggg tatcgcgtcg ccctcttggt ggcgggcagc ccggacttcg
     3061 ggctctcgac cctcttcgag atgagcgcac acgcgatgcc gttcgcggtg cgcgtgttcc
     3121 gcagccgcac ggaagcgacg cgttggctcg cgggccagga cgactgacgc aatgacgcgg
     3181 tggtttcgcg cgggagcgat cgggctgttg gtggtggcgg tgctccacac cttcggccac
     3241 ttcggcgggg gacccaagga tcccgcgcgc atccagatcg aggaagcgat gaacgcctat
     3301 cgcttcgaca tcatgggaat gcaccccagc gcgagcgata tcaccaacag cttgagcctc
     3361 atgatgacga tcatgctcgt cttcgcgggc gtgctcgatc tgatcgtggt gggcgcgctc
     3421 tccggcaacg gccgcgtgct gcgtcgtctc gcgtgggtga acgcggtggg cgtgggcgcg
     3481 ctggcggcgt tgttcgcgta ctatcgcatc ccgcccccga tggcgacgct cgcggtcgta
     3541 tgcctcttct tcgtggtggc cgcgcttcga cccgctgcac ggcggttcta accccagggg
     3601 ttggcaggcg cggggtcgcg tgctagactt cgggccatga agtcgcaggt gggcgtgtcc
     3661 gacaaaccga ccatttcgac caaggcgccg gtcagtgcgt tcttgcgcca tcatttccgc
     3721 cacttcaacg cggctgcctt gatcgacgcg gccgacgcct accgccgcca cctcgacggc
     3781 ggcggcaaga tgatggtgac cgtcgcgggt gccatgagca ccgccgagct cggcatctcg
     3841 ctggccgaga tgatccggcg cgacaaggtc catatcatct cgtgcacggg cgcgaatctc
     3901 gaagaggacg tctacaatct catcgcgcac gacttctacg aacgcattcc acactatcgc
     3961 gacctgaccc cgcacgacga gaagcgcctg ctcgatcgtc acctgaaccg ggtgacggac
     4021 acgtgtatcc ccgaggaaga agcgatgcgg cgcctggagg ccgtggtact cgaagagtgg
     4081 tcgcgcgccg acgccaaggg cgagcggtat ttcccgcacg agttcatctt caaggcgatc
     4141 cgctccggcg ctctcaagca acactaccag atcgatccca aggactcgtg gatcgtggcc
     4201 gcggcggaga agaacctccc catggtggtg ccgggctggg aggactcgac cctgggcaac
     4261 atgttcgccg cgtcgctgat tcgcggcgac gtcaagaagg cgaccacgat gcgttccggt
     4321 gtggagtaca tggtcgactt ggcgaattgg tatcaaagca cggccgcgac gtcgtcgatc
     4381 gggttcttcc agatcggagg cggcatcgcg ggcgacttcc cgatctgcgt ggtgccgatg
     4441 ctcgagcagg acctcggcga gcgcaccccg ctctggggct acttctgcca gatcagcgac
     4501 tcgac
//
LOCUS       DASPGK010000234         7916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_411857, whole genome shotgun sequence.
ACCESSION   DASPGK010000234 DASPGK010000000
VERSION     DASPGK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411857"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1116)
                     /locus_tag="VEC56_06395"
     CDS             complement(61..1116)
                     /locus_tag="VEC56_06395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV13818.1"
                     /translation="MTPRDEWLLAIRLRQLGDVLAALATLQALKAHRPERRIAYVVEA
                     PYADLLRGLDFIDALPSPPRGGASAWRAYLASLRRLHPTAAIDFHGSARSALMAWISG
                     APTRVGFDVRGRRAAYTHVVPRGEFRDGVRVPHTPIEWGMRLAQHVGVGTAEGLPPRL
                     AVSDAARARAREALSGQGISRGELDARALVGVNPGRPAPVKSWDPARFAQLARRLAGS
                     GRRALVFWGPGEEETARAIVRDAGTAAVLAPATPLAGMPALLDACGALVTIDSGLKHL
                     AVCVRVPTVTVFGATDPREWHMGGGADAVLWRGLSCSPCRRTACPFGAPCMDVSVDAV
                     WSATERVLGAVAREARA"
     gene            complement(1113..2171)
                     /locus_tag="VEC56_06400"
     CDS             complement(1113..2171)
                     /locus_tag="VEC56_06400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV13819.1"
                     /translation="MMPAPELHPGDRVLVTRLDYLGDVVLSLPLVDALRARWPGLEVD
                     YLTRTPAADLLFRDARFARVWTQTNGARATASLVRALRARRYRAVIDLYSNPRSAWLA
                     WSTGARVRVGGNRRGRRRLYTHPVVVSREVRRVTDVFVRYGIPLGLDAGQPASRPSLV
                     IHRDEAAAADELLARHAGGAEAASRRRIGVHPGGKWSVKRWPTEKFVELIRILEHEMD
                     GSVVVFTGPGERDATERVRALASGRAVFLDVLPIRTAAAVISRLDAMVACDGGIMHVA
                     AAVGTPTVGIFGSSEPAVWFPYAGAGPYRAAYIDVECRPCHRHECPLGHTRCLNELSA
                     EAVAGCVRAAMAQAEVSP"
     gene            complement(2168..2668)
                     /locus_tag="VEC56_06405"
     CDS             complement(2168..2668)
                     /locus_tag="VEC56_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXV13820.1"
                     /translation="MKQVGARVFSDLAALRAFVAEARARRVVLANGCFDPLHVGHTRY
                     LTDAKARGDFLVVAVNDDAGARRLKGAGRPVVAAVDRARLVAALACVDAVFVFGDDDV
                     ARILEALRPHVHAKGTDYTSETVPERGVAERLGIETVITGDPKTHASREVLARLRAPE
                     PGEPTK"
     gene            complement(2665..3672)
                     /locus_tag="VEC56_06410"
     CDS             complement(2665..3672)
                     /locus_tag="VEC56_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXV13821.1"
                     /translation="MIDRFQDLLDAFTTRRIAVVGDFLLDQYMLGTTARVSREAPIVV
                     VDYQETVNHPGGAANAAQNVTALGGTAVAVGVLGADREADALVTLLRERGVDTNGLLA
                     LARATTSLKLRIMAGEMNAQKQQVARVDRSHRIELDAAARAALVEAVESAGGSCDAVL
                     LADYGLGVLGEDVSARAIDVARARGVPVVVDSRYRAFAFRGATVATPNEVEAMAALGL
                     EREGDLMDGERLRRSLAQTGLASLIVTRGSKGMVVCDEGGKVSTIGIAGTHQATDVTG
                     AGDTVSAVVALSLSAGASLEEAARMATFAASIVVMKRGTATASPAEMRTVIESERRAN
                     A"
     gene            complement(3761..4801)
                     /gene="waaF"
                     /locus_tag="VEC56_06415"
     CDS             complement(3761..4801)
                     /gene="waaF"
                     /locus_tag="VEC56_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HXV13822.1"
                     /translation="MSANAGSPRRLVMVAPNWLGDAVMSLAAVDRVARSSGWELTVVA
                     SPYTARVYWGVDGVHEIQADAPGGRAPRIRDRARALRAYGADTVVVLPPSFSSAVPAW
                     LARVRVRVGWASDGRRALLTHAVAVAPRTTHLSTGYADLMDHALAGAASAEPRVEGAA
                     APRARLRVGDEERSRLDRVLARFGVSRGSYVLVVPGAAFGPAKSWPWERYRRVCEALA
                     RDTPVVLSGSAGDRAVCAQIRDGLQGVADLAGETTLGEFFALVEGARAVLANDSGAPH
                     VAASLAVPTVVLFGSTSPEWTAPVGERVRVLQHKVHCNPCFRRTCPTQLECFNGIDPA
                     DVLANVRALLSS"
     gene            complement(4798..6639)
                     /locus_tag="VEC56_06420"
     CDS             complement(4798..6639)
                     /locus_tag="VEC56_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV13823.1"
                     /translation="MSTYRRLLAYVRPYWPRMLVLSLTIMMFASLSGVSLTLIPPFLR
                     VILERDAPAPSLPGETSEGLPLPRFVEETRVEVKGAFEGWMYAGDADERLARFIWLLI
                     ALMIAKNIFGYVQTYMTEYLEQRVLYRIRRDVYAHILDLPLSYFDRERAGHLISKVTN
                     DVSMLRGAVIGVAASVLRNALMTLLALTILLTVSWRLTLLVLCVVPLNVLLINWVGKK
                     LKRRSRRAQEGMADMTASLEETIAGVRVVKAFDTRSHEQERFGRHSYRHMMHYIRMQM
                     YGALSAPTSELLGTVSFAIVLWYGGQRVITGGLPAENLVMFVAAMLFVVSPLKNLSRL
                     STVVQQANASAQRVFDLLDVAKEHAVEGRGVAAFENEVRFENVSFEYVAGEPVLREVS
                     FVAKKGEVVAIVGSSGAGKTTLVDLIPRFYPPTAGRVTIDGVDTRDVSLSSLRALMGI
                     VTQETILFNDSIYNNIAYGSPNATQEEIERAARAANAHDFIVEFPDGYDTVVGDRGAQ
                     LSGGQRQRIAIARAILRDPAILIFDEATSALDSESERLVQEAMERLFAGRTTFVIAHR
                     LSTIRHADKILVLERGRLTEHGTHAELMTKNGTYHRLYQLQFGHANA"
     gene            complement(6700..7794)
                     /locus_tag="VEC56_06425"
     CDS             complement(6700..7794)
                     /locus_tag="VEC56_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV13824.1"
                     /translation="MSSTTLTHRRDQMVGMLTPLFSRMVGQASFSLPGDINNQSRVLV
                     VDSGDLTELLFFAPVLNHLKRRYPGMRVTVLVREGNGELIRTLEPINEMISYEPEHLS
                     LFSSTYFALLRRIKTRGFDVVFLLGHEFSLARSLVVLMSKARLRVGFQQKFTYPFVNC
                     EIRASRKVDYEAPRALSYLSVLGQSPGDSIPAWKLPEADVRWAAQMIHFRSPNKDTML
                     IAVDPGMGKGNHRLVETAFAHVVNEVARRHGGKILLLSNNLDPKGMARFRALVKASVI
                     DVEPRNVKEALALLSRASLVLAGNTDFFHFAVSMRLPTIGFFTRFDAANWFPKSTPWV
                     QIIQGVRGQHVSVDEVCSKIDTLLHLAQPR"
BASE COUNT         1282 a         2822 c         2682 g         1130 t
ORIGIN      
        1 agcgggcgag gttcgcttcc tcgttgaacg cggtcaccac cacgctgatg cgaccgaggt
       61 tcacgcgcgc gcttcccttg cgaccgcgcc cagcacgcgc tcggtggcgc tccacaccgc
      121 gtcgaccgac acgtccatgc agggcgcccc gaacgggcac gccgtgcggc ggcacggcga
      181 gcacgagagt ccgcgccaca acaccgcgtc cgcgccgccg cccatgtgcc actcgcgcgg
      241 gtcggtggcg ccgaacacgg tcacggtcgg cacccgcacg cacaccgcga ggtgcttcag
      301 cccggaatcg atggtgacga gcgcgccgca ggcgtcgagc agagccggca tgccggccaa
      361 cggtgtggcg ggtgccagca ccgcggccgt acccgcgtcc cgcacgatgg cgcgcgcggt
      421 ctcctcttcg cccggccccc aaaacaccag cgcgcgtcgc ccactccccg cgaggcgtcg
      481 cgccagttga gcgaaacgag ccggatccca cgacttgacg ggcgcggggc gacccgggtt
      541 cacccccacc agcgcgcgcg cgtccaactc gccgcgcgag attccctgcc ccgagagcgc
      601 ctcgcgcgcc cgcgcgcgcg cggcgtcgga gacggcaagg cgcggcggca atccttccgc
      661 cgtacccacg ccgacgtgct gcgccaagcg catgccccac tcgatcggcg tgtgcggcac
      721 gcgcacgccg tcgcgaaact cgccgcgcgg caccacgtgg gtgtaagccg cgcgccgacc
      781 gcgcacgtcg aagccgacgc gggtcggcgc ccccgagatc cacgccatca gggccgagcg
      841 cgcgctgccg tggaagtcga tcgccgccgt cgggtgcagg cgcctcagcg aggccagata
      901 cgcgcgccac gcactcgccc cgccgcgtgg cggcgatggc agcgcatcga tgaagtcgag
      961 cccgcgcagc agatccgcgt atggtgcttc gaccacgtaa gcgatgcgtc gctccgggcg
     1021 atgcgctttc aacgcctgca gcgtggccag tgccgccagc acgtcgccga gctggcgcag
     1081 ccgaatcgcg agcagccact cgtcgcgcgg cgtcatgggg acacctccgc ctgcgccatc
     1141 gcggcgcgca cgcagcccgc caccgcttcg gccgagagct cgttcaaaca gcgcgtgtgg
     1201 ccgagcgggc actcgtggcg gtggcagggc cggcactcga catcgatata ggcggcccta
     1261 tacggtcccg cgcctgcata cggaaaccac accgccggtt cgctgctccc gaagataccc
     1321 actgtcggcg ttcccaccgc cgcggccacg tgcatgatgc cgccgtcgca agcgaccatc
     1381 gcgtccaagc gcgagatcac agcggccgcg gtgcgaatgg ggagcacgtc caggaacacg
     1441 gcgcgtcccg aggccagcgc gcgcacccgc tcggtggcgt cgcgctcgcc gggtcccgtg
     1501 aacaccacca cgctcccgtc catctcgtgc tcgaggatgc gaatcaattc gacaaacttc
     1561 tccgtcggcc agcgcttgac cgaccacttg ccgcccgggt ggaccccgat gcgcctgcgg
     1621 gacgccgcct cggcgccacc cgcgtgacgc gcgagcaact catccgcggc ggccgcttca
     1681 tcgcgatgga tgaccagcga cggacgagac gcgggttgcc ccgcatcgag accgagcggg
     1741 attccatagc gcacgaacac gtcggtgacg cggcgcacct cgcgcgacac gacgaccggg
     1801 tgggtgtaca accgtcgccg gccgcgccgg ttgcccccca cgcgcacgcg cgcgccggtc
     1861 gaccacgcaa gccacgcgct cctcggattc gagtagaggt cgatcaccgc gcggtagcgt
     1921 cgcgcccgca gcgctcgtac cagcgacgcc gtcgcgcgcg cgccgttggt ctgcgtccac
     1981 acccgcgcaa agcgcgcgtc gcggaacagg aggtcggcgg cgggtgtgcg ggtgagatag
     2041 tcgacttcca gcccgggcca gcgcgcgcgc aacgcatcca ccaaggggag tgaaagcacc
     2101 acgtcgccca ggtagtccag tcgcgtcacc agcactcggt caccggggtg cagctccggc
     2161 gcgggcatca tttcgtgggc tcacccggct ccggcgcgcg cagacgagcg agcacctcgc
     2221 gcgacgcgtg tgtcttgggg tcgcccgtaa tcaccgtctc gatccccagc cgctcggcca
     2281 ccccgcgctc cggtaccgtc tccgatgtgt agtcggttcc cttcgcgtgc acgtggggcc
     2341 ggagcgcctc caggatgcgc gcgacgtcgt cgtcgccgaa cacgaacacc gcgtcgacgc
     2401 acgcgagcgc ggccaccaag cgggcccggt cgacagccgc gaccaccggg cgtcccgcac
     2461 ccttcaatcg gcgggcgccc gcgtcgtcgt tgacggccac gaccaagaaa tcgccgcgcg
     2521 ccttggcgtc ggtgaggtac cgtgtgtgcc ccacgtgcaa cgggtcgaag cacccgttcg
     2581 cgagcacgac gcggcgcgcg cgcgcttcgg cgacgaacgc gcgcagcgcc gccaggtcgg
     2641 aaaaaacacg cgcgcccact tgcttcacgc gttcgccctt cgctccgatt cgatcacggt
     2701 gcgcatctcc gccggcgacg cggtcgcggt cccgcgcttc atcaccacga tggaggccgc
     2761 gaaggtcgcc atccgcgccg cttcctcgag tgatgcaccg gcggagagcg agagcgcgac
     2821 caccgcggaa acggtgtcgc ccgcgccggt cacgtcggtg gcctggtggg tacccgcgat
     2881 gccgatggtg gaaaccttcc cgccctcgtc gcacacgacc atgcccttgc tgccgcgggt
     2941 cacgatcagg ctggcgaggc cggtctgggc gagcgaccgt cgcaagcgct ccccgtccat
     3001 gaggtcgcct tcccgctcca gaccgagcgc ggccatcgct tccacttcgt tgggtgtggc
     3061 gacggtggcg ccgcgaaacg cgaacgcccg gtagcggctg tccaccacga ccggcacccc
     3121 gcgcgcgcgg gctacgtcga ttgcgcgcgc cgacacgtcc tcgcccagta cacccaagcc
     3181 gtagtcggcg agcaggacgg cgtcgcacga cccgccggcg gactccaccg cttcgaccag
     3241 cgcggcccgg gccgccgcgt ccaactcgat gcggtgcgag cggtcgacgc gcgccacctg
     3301 ctgcttttgc gcgttcattt cgcccgccat gatgcgcagc ttgagcgacg tcgtcgcgcg
     3361 cgcgagcgcg agcagcccgt tggtgtccac gccccgctcg cgcagcaacg tgaccagcgc
     3421 gtcggcttcg cggtcggcgc ccagcacgcc caccgcgacc gcggtccccc ccaaggcggt
     3481 gacgttctgg gccgcgttgg cagccccgcc cgggtggttc accgtctctt ggtagtcgac
     3541 caccacgatg ggcgcttcgc gcgacacgcg cgccgtcgtc ccgagcatgt actggtcgag
     3601 gagaaaatcg ccgaccacgg cgatgcgccg cgtggtgaag gcgtcgagaa ggtcttggaa
     3661 tcgatcgatc atgacctttg gcggctgcgc gccgcgttcg cgatagtagc ccaggcacgc
     3721 ccctacgggc gacgggtttt atccgcggcg gtagccgggt ctacgaactc agcagcgcac
     3781 gcacgttcgc gagcacgtcg gcggggtcga tgccattgaa gcactcgagc tgcgtggggc
     3841 acgtgcgccg aaaacacggg ttgcagtgca ccttgtgctg gaggacgcga acccgttcgc
     3901 ccaccggtgc cgtccactcg ggagacgtcg agccgaacag gaccacggtc ggtaccgcca
     3961 gcgacgcggc cacgtgcggg gcgccgctgt cgttggcgag gaccgcgcgc gcgccctcca
     4021 cgagtgcaaa gaactcgccc agcgtggtct cgcccgcgag gtcggcgacc ccctggagcc
     4081 cgtcgcggat ctgcgcgcac accgcgcggt cgccggcgct ccccgagagc acgaccggtg
     4141 tgtcgcgcgc gagcgcttcg cacacgcgcc ggtagcgctc ccacggccac gatttggcgg
     4201 ggccgaacgc ggctccggga accaccagga catacgaccc gcgcgatact ccgaagcgcg
     4261 cgaggacgcg gtcgaggcgc gagcgctctt cgtcaccgac gcgcaggcgc gcgcgcggcg
     4321 cggccgcccc ctcgacgcgc ggctccgcgg aggccgcgcc cgcgagcgcg tgatccatca
     4381 ggtccgcgta gcccgtgctc aagtgcgtcg tacgcggcgc caccgccacc gcgtgcgtca
     4441 agagtgcgcg cctcccgtcg ctcgcccacc ccacgcgcac gcgcacgcgt gcgagccacg
     4501 ccggcaccgc gctcgagaac gagggcggca ggaccacaac ggtgtccgcc ccgtaggcgc
     4561 gcagcgcgcg cgcccggtca cggatgcgag gcgcgcgccc gccgggcgcg tcggcttgaa
     4621 tctcgtgcac gccgtcgacg ccccaataga cgcgcgccgt gtatggcgac gctaccaccg
     4681 tgagctccca gccgctcgat cgcgcgaccc gatcgaccgc agccaggctc atgacggcgt
     4741 cgccgagcca attgggcgcc accatcacca ggcgccgggg cgatccggcg ttcgcgctca
     4801 tgcgttggcg tggccgaatt ggagctggta gaggcggtgg tacgtgccgt ttttggtcat
     4861 cagctcggcg tgcgtcccgt gctcggtcag gcggccgcgc tcgaggacga gaatcttgtc
     4921 ggcgtgacga atggtcgaca gacggtgggc gatgacgaag gtggttcgcc ccgcgaacaa
     4981 ccgctccatc gcttcctgga ccagacgctc gctctcgctg tcgagtgcgc tggtggcttc
     5041 gtcgaagatg aggatggcgg ggtcgcgcag gatggcgcgc gcgatcgcga tgcgctggcg
     5101 ctggccgccc gagagctgcg cgccccgatc gcccaccacg gtgtcgtagc cgtcgggaaa
     5161 ctccacgatg aagtcgtggg cgttggcggc gcgcgcggcc cgctcgatct cttcctgggt
     5221 ggcgttgggg ctcccgtagg cgatgttgtt gtagatggaa tcgttgaaca ggatcgtctc
     5281 ctgggtgacg atgcccatca gtgcgcgcag ggacgacaac gagacgtcgc gcgtgtccac
     5341 tccgtcgatg gtgacgcggc ccgcggtggg cgggtagaag cgcggaatca gatcgaccag
     5401 ggtggtcttg ccggccccgc tcgaccccac gatggcgacc acttcgccct tcttggcgac
     5461 gaagctcacc tcgcgcagca ccggctcgcc cgccacgtac tcgaacgaca cgttctcgaa
     5521 gcgcacctcg ttctcgaacg cggccactcc gcggccctcg accgcgtgct ccttggccac
     5581 gtccaggagg tcgaacacgc gctgcgccga cgcgttggcc tgctgcacca ccgtgctcag
     5641 ccggctcaag ttcttgagcg ggctgaccac gaacagcatg gccgccacga acatcaccag
     5701 attctcggcc gggagaccgc ccgtgatcac acgctgtccg ccataccaga gcacgatcgc
     5761 gaacgacacc gttcccaaca actccgaggt cggggcagag agcgcgccgt acatctgcat
     5821 gcgaatgtag tgcatcatgt gccggtagct gtggcgtccg aagcgttcct gctcgtgcga
     5881 gcgcgtgtcg aaggccttga ccacgcgtac gcccgcgatg gtctcctcga ggctcgcggt
     5941 catgtcggcc atcccctcct gcgcgcgacg gctgcggcgc ttgagcttct tccccaccca
     6001 gttgatcaag agcacgttga gcggcaccac gcacagaacc agcagtgtca gccgccacga
     6061 caccgtcagg agaatggtga gcgcgaggag cgtcatgagg gcgttgcgca gaacgctcgc
     6121 ggccaccccg atcaccgcac cccgcagcat ggagacgtcg ttggtgacct tggagatcaa
     6181 gtggcccgcg cgctcgcggt cgaagtagga caagggcaag tcgaggatgt gggcgtaaac
     6241 gtcgcgacgg attcgataca gcacgcgctg ctccaggtac tcggtcatgt acgtctgcac
     6301 gtacccgaag atattcttgg cgatcatgag cgcgatcaag agccagatga aacgcgccag
     6361 gcgctcgtcg gcgtcgccgg cgtacatcca gccctcgaag gcgcccttga cctcgacgcg
     6421 ggtctcctcg acgaagcgcg gcagcggcag gccttcgctc gtctcacccg ggagcgaggg
     6481 ggcgggcgcg tcgcgttcca agatgacgcg caggaagggc gggatcagcg tcagcgacac
     6541 gcccgagagc gacgcgaaca tcatgatcgt cagcgagagg accagcatgc gaggccagta
     6601 cggccgcacg taggcgagca ggcgtcggta ggtactcatg gaatcacgcc cggggcgtgc
     6661 cgggacgagg gcaaatatgg gggctcggga ccgcgcgcgc tagcgtggct gcgccaggtg
     6721 gagcaaggtg tcgatcttgg aacacacttc gtccacggat acgtgctgcc cgcggacccc
     6781 ttggatgatc tgcacccacg gcgtggattt tggaaaccag ttggcggcat cgaagcgcgt
     6841 gaagaacccg atcgtcggca agcgcatgct caccgcaaag tggaaaaagt cggtattgcc
     6901 cgccaagact aggctcgcgc ggctcaagag cgcaagcgct tccttgacgt tgcggggctc
     6961 gacgtcgatc accgaggcct tgaccagggc gcggaagcgc gccatcccct tgggatcgag
     7021 gttgttcgac aagaggagga tcttgccccc gtggcgacgc gccacctcgt tgaccacgtg
     7081 ggcgaaggcg gtctccacca agcggtggtt ccccttgccc atgcccgggt cgaccgcgat
     7141 cagcatggtg tccttgttgg gactgcggaa gtggatcatc tgcgcggccc accggacgtc
     7201 ggcctcgggc agcttccacg ccggtatcga gtctcccggg ctctgcccca gcacgctcag
     7261 gtatgacaag gcgcgcggtg cctcgtaatc gaccttgcgc gacgcgcgga tctcgcagtt
     7321 gacgaacggg tacgtgaact tctgctggaa gccgacgcga agccgtgcct tcgacatgag
     7381 taccaccagc gagcgcgcca ggctgaactc gtggcccagc aagaacacca cgtcgaaacc
     7441 ccgcgtcttg atgcgacgca agagcgcgaa gtacgtgctc gagaatagcg agaggtgctc
     7501 gggttcgtag ctgatcatct cgttgatggg ctcgagggtg cggatcagct cgccgttgcc
     7561 ctcgcgcacc agcacggtga cgcgcatgcc ggggtagcgc cgcttgaggt gattcaagac
     7621 gggggcgaag aagagcagtt cggtgaggtc cccggagtcc acgaccagca cccggctctg
     7681 gttgttgatg tcgccgggga gcgagaagct ggcctggccg accatgcgcg agaacagcgg
     7741 ggtcagcatg ccgaccatct ggtcgcggcg gtgggtcaga gtcgtcgagg acacgctgga
     7801 atctccgtcg gaatccgggc tcgagtcgcc ggggaatagg cgtaactaca gcaaaacaag
     7861 ggccaatcac ttccacagga tggtcttgtc gatcgcctcc gaggcccggt ggatgg
//
LOCUS       DASPGK010000235         6226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_763239, whole genome shotgun sequence.
ACCESSION   DASPGK010000235 DASPGK010000000
VERSION     DASPGK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763239"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /gene="leuS"
                     /locus_tag="VEC56_06430"
     CDS             <1..2153
                     /gene="leuS"
                     /locus_tag="VEC56_06430"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXV13825.1"
                     /translation="QFHHWGVVYDWTREVASCEPEYYRWTQWLFLELYRAGLAYRKAA
                     SVNWCPKDKTVLANEQVVDGRCERCGTEIEERHLDQWFFRITAFADALLDDLGTLADW
                     PERVVTMQKNWIDKSVGVEIEFEIQGASHSVNVFTTRPDTIFGATFLVLSPNHPMVEN
                     LIAGSGRETELRSVISRWTADRLSGKARTEPSKEGVFLDTHAINPMTREPVPVYMAPY
                     VLMEYGSGAIMAVPAHDQRDFEFATRYGLPIREVVRSANGATTLPTSSYSGDGTLINS
                     GAFDGLGQGEAFAKIAAQLERDGKGKRRVHYRLRDWLISRQRYWGAPIPMIHCPKCGV
                     VPVPDDQLPVRLPDDVDFHPRGDGKSPLATSDAFRTVACPTCGGAAERDTDTMDTFVD
                     SSWYFLRYLSPRDDRVAFRKDVCDAWLPVDQYIGGVEHAILHLLYARFIVKFLHSRGH
                     VSFAEPFARLFTQGMVCKNGIKMSKSKGNVVPPDTIITPMGADTMRLYILFCGPPERD
                     TEWNDEAVEGCYRFLNRVWRHFEANAHALAKPFAESIDPTALDDAERALFRKTHQTIA
                     RVVADVNDHFHFNTAISALMELSNAMDDFMTAGGRAASAAFRFAFDVLIRLLAPMTPH
                     LSEELWERTGHGRSIFSAALPVADPAYTRDESFDLVIQVNSKIRARETVPADTDAERM
                     KAIAMAHPRIQEILEGRTPKRVVVIQNRLVNIVI"
     gene            complement(2297..2680)
                     /locus_tag="VEC56_06435"
     CDS             complement(2297..2680)
                     /locus_tag="VEC56_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13826.1"
                     /translation="MAAPTVSNSERTTPSDAERWYAAVGYLFFMCFLSLWKSKESRFV
                     RFHARQAFLLFLAECVAFVAIAVIDRTVGRLPFLGLIVVVIAQVFVYLTALFLSVMGF
                     VKALFGERWEMPFFGPYREKIPPLS"
     gene            complement(2742..3410)
                     /locus_tag="VEC56_06440"
     CDS             complement(2742..3410)
                     /locus_tag="VEC56_06440"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13827.1"
                     /translation="MSATATKRARKQIREDKLVTTTVRFSEWAQAHFNQVIIGIVALV
                     AVVAVLVFAANSRENNARQAERVMGSALALMLQGDYTAAKSSFQQVSERFGGKQGVAA
                     RFFEAECGAKQGNFEEALADYDAYLAASSEYPEFENAALIGKALCLEGMKNYVEAAPA
                     MAAAVAKLDPKDPRYADAALNAGDLYTVAGNPREALTYYRLASEHGTGDVKARATVGV
                     ELTQ"
     gene            3605..6124
                     /locus_tag="VEC56_06445"
     CDS             3605..6124
                     /locus_tag="VEC56_06445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXV13828.1"
                     /translation="MTIPHPHEPRRRPSLYGDRAAPWARPLLFVFVIATVVLAFPGSR
                     ERFQSPYSGIQARNLVVQSVREDGPNAGADLRAGDELFAIDGERLRNGAHYQHVVAAN
                     RSFRPQSYEIRRHGVVLQVPIEYQPIPRMLAAERVVLIVLALAFLVGGLWVYFRRSDV
                     VGTLFAFNSAILASFLTDGPSTGNPALQLSAEIVGDAIILAFPATLLHFFLRFPDRGP
                     RESGARRRAWLYIPPAVIFAASAVVSARRFRFVTHEVEEQVVLALSTAYFVAYVLSSL
                     VVFVRAYRAAPRAQKQKLRVVIAGTVAGLLPFLAATVHASIRPEAGAMSFTLPAELCL
                     GFVPIAFAYAILKHGAIELTSVVRKSLVYALLTGLLIATYYALVQTAGAFLSREFGVS
                     EAVWGTIAVVVLAIGFAPVRERVQGVVDRIFYRAEYVYKQEVIDFGRQVARTLTREEM
                     FDHFIARCDELLHPTFVVVYLRGEDKDLGLERVHGAAPEMNPTFTADSFLGRYFTRYR
                     TPLLVEFLDRSWERPNLDPEARRLLSLPGLAACVPIAAPDRLLGVALLGQKRSGTAYR
                     RADAELLETFADQLALVVENADLIHSSIEKERLKSEVMLARDIQQALLPATAPRLRDV
                     EIRGQMISSSEVGGDYFDYFMLDERRIVVAIGDVMGKGVPAAMLMASLQAVFKNRAAK
                     GGLAPHELNRELNDYLVEHAKPGQFATFFCGVLDLAHSTFTFSSAGQCPALLATNGFI
                     DRLGNGGTVLGANPNQDYAEGTVAFHPGDLLILYTDGVIEQMNDQGEPYGEQRLVEFV
                     EANRNLPPDRLQNALLADVLAFGGGRQDDDITSVIAVRRSA"
BASE COUNT         1008 a         2129 c         2017 g         1072 t
ORIGIN      
        1 agcagttcca ccactggggc gtggtctacg actggacgcg cgaggtggcg tcgtgcgagc
       61 cggagtatta ccgctggacg cagtggctgt tcttggagct ctaccgcgcc ggcctcgcgt
      121 accgcaaggc cgcttcggtc aattggtgcc cgaaggacaa gaccgtgctc gccaacgagc
      181 aggtggtcga cggacgctgc gagcgctgcg gcaccgagat cgaagagcgt cacctcgacc
      241 aatggttctt ccgcatcacc gcgttcgccg acgcgctact cgacgacctg ggcacgctgg
      301 ccgactggcc cgagcgcgtg gtcacgatgc agaagaactg gatcgacaag agcgtcgggg
      361 tcgagatcga attcgagatc cagggcgcga gccattccgt caacgtgttc acgactcggc
      421 ccgacacgat ttttggcgcc acgtttctcg ttctatcccc gaatcatccg atggtggaga
      481 acctgatcgc cggcagcgga cgcgaaaccg aacttcgttc ggttatctcg cgctggaccg
      541 cggaccgctt gagcggcaag gcgcgcaccg agcccagcaa agagggcgtc ttcctggaca
      601 cgcacgcgat caatccgatg acgcgcgagc cggtacccgt gtacatggcg ccctacgtgc
      661 tgatggagta cgggagtggc gccatcatgg cggtgcccgc gcacgaccaa cgcgacttcg
      721 agtttgcgac ccgctacggc cttcccatcc gcgaagtcgt gcgctccgcg aacggcgcca
      781 cgacgctccc gacgtcgtcg tattccggcg acggcacgct gatcaacagc ggcgccttcg
      841 acggcctcgg gcaaggcgag gcgttcgcga agatcgcggc ccagttggag cgggacggaa
      901 aagggaagcg gcgcgtgcac taccggctgc gcgactggct gatctcgcgc cagcgctact
      961 ggggcgcccc catcccgatg atccactgcc cgaagtgcgg tgtggtcccg gtgccggacg
     1021 accagctgcc ggtgcggctg cccgacgacg tcgacttcca cccgcgcggc gacggcaaga
     1081 gcccgctcgc gaccagcgac gcgttccgca cggtggcgtg tccgacgtgc ggcggcgctg
     1141 ccgaacgcga caccgacacc atggacacct tcgtcgactc gagctggtac ttcctgcgct
     1201 acttgagccc gcgcgacgac cgcgtcgcat tccgcaagga cgtgtgcgac gcgtggctgc
     1261 cggtcgacca gtacatcggc ggcgtcgagc acgcgattct gcatctactg tacgcccggt
     1321 ttatcgtcaa gttcttgcat tcgcgcggcc acgtgtcgtt cgcggagccg ttcgcgcgtc
     1381 tcttcaccca gggcatggtg tgcaagaacg gcatcaagat gtcgaagagc aagggtaacg
     1441 tggtgccgcc ggacaccatc atcacgccca tgggtgcgga caccatgcgg ctctacatcc
     1501 tgttctgcgg gccgcccgag cgcgacacgg agtggaacga cgaagccgtc gaggggtgct
     1561 atcggttcct gaatcgcgtg tggcgccact tcgaggccaa cgcgcacgcg ctggcgaagc
     1621 cgttcgccga gtcgatcgac cccacggcgc tggacgacgc ggagcgagcc ctgtttcgaa
     1681 aaacccacca gaccatcgcg cgcgtggtgg ccgatgtgaa cgaccacttc catttcaata
     1741 ccgcgatcag cgcgctcatg gagttgtcca acgccatgga cgatttcatg acggccggtg
     1801 ggcgcgccgc ctcggcggcg tttcgcttcg ccttcgacgt cctgattcgc ttgctggccc
     1861 cgatgacgcc gcacctgtcc gaagaactct gggagcggac cggtcacggg cgcagcatct
     1921 tctcggccgc actgccggtc gccgacccgg cctacacgcg cgacgagagc ttcgacctgg
     1981 taatccaggt caacagcaag atccgcgcgc gcgagacggt gccggccgac accgacgccg
     2041 agcgcatgaa ggcgatcgcg atggcgcacc cgcgtatcca agagatcctg gaaggacgca
     2101 cacccaaacg ggtggtcgtc atccagaacc gcctggtcaa catcgtcatc tagaagagtg
     2161 ctcgttcgga gcgccaggcc ccgagcccgc ttcacgccgg cctgggttaa cataatgtat
     2221 cttatgcgcc acacggtgcc gggctcgcgc cgaccccggg gtccgctgcc cggtcttccc
     2281 cggcggtatc gaacggctac gaaagcggcg ggatcttctc gcggtacggg ccgaagaacg
     2341 gcatctccca gcgctcgccg aagagcgcct tcacgaaccc catcacggac aggaacaacg
     2401 ccgtgaggta cacgaagacc tgggcgatga cgaccacgat caggcccagg aacgggagcc
     2461 tccccaccgt gcgatcgata accgcgatgg cgacgaaggc gacgcactcg gcgaggaaca
     2521 ggaggaaggc ctggcgggcg tggaagcgaa cgaaccggct ctccttcgac ttccacagcg
     2581 agaggaagca catgaagaac aggtagccga cggccgcgta ccaacgctcg gcgtcggatg
     2641 gcgtcgtcct ctcggaattg gaaacggtgg gtgcggccac ggtaatacgg cgcgctttgc
     2701 gttcgaaatc gtgtccccgg cgcgacgctc gctccggagc tttactgggt cagctcgacg
     2761 cccaccgtcg cgcgcgcctt gacgtcgccc gtcccgtgct cggacgcgag ccggtagtac
     2821 gtgagcgctt cgcgcggatt gccggcgacg gtgtacaggt cgcccgcgtt gagcgcggcg
     2881 tcggcgtagc gcgggtcctt cgggtccagc ttggcgaccg ccgccgccat cgcgggcgcg
     2941 gcttcgacgt agttcttcat gccctccagg cacagcgcct tgccgatcaa cgcggcgttc
     3001 tcgaactcgg ggtactcgga actggccgcc agataggcgt cgtagtcggc gagcgcctcc
     3061 tcgaagttcc cttgcttcgc cccgcactcc gcttcgaaga agcgcgcggc cacgccttgc
     3121 ttgccgccga accgctcgga cacttgctgg aagctcgact tggcggcggt gtaatcgccc
     3181 tggagcatca gcgccagcgc ggaccccatc acgcgctccg cctggcgcgc gttgttctcg
     3241 cggctgttgg ccgcgaagac cagcaccgcc accacggcca cgagcgccac gatgccgatg
     3301 atgacctggt tgaagtgggc ctgggcccac tcgctgaacc ggaccgtggt ggtgaccagc
     3361 ttgtcttcgc gtatctgctt gcgtgccctt ttggttgcgg tcgcgctcat gtggacaata
     3421 tcgctcctgt ggaaatagat cgtaattcgt ttagatttgg tagactagca cacggcgcgc
     3481 gcacgaacaa ggggatcctc ccggtcgcac gcggcacggg acgccctgga aagacgttga
     3541 cacggtctgg agggcgtccg cacgctatac tctccgctcg tccccatcga ccggcggagg
     3601 cagcatgacg attcctcacc cccacgaacc gcgccggcga ccgtcgctct acggcgaccg
     3661 ggccgcccct tgggcccgtc cgttgctgtt cgtgttcgtg atcgcgacgg tcgtgctcgc
     3721 tttcccgggc agccgcgagc gattccaatc cccctactcg ggcatccagg ctcgcaacct
     3781 cgtcgtccag tcggtacgcg aagacggccc caacgcgggc gccgacttgc gcgccggcga
     3841 cgagctcttc gcgatcgacg gcgagcggct gcgcaacggc gcgcactacc agcacgtggt
     3901 ggcggcgaat cgctccttcc gcccgcagtc ctacgagatt cgacgccacg gggtcgtgct
     3961 gcaagttccg atcgagtacc agccgatccc ccgcatgctc gccgccgaac gcgtggtctt
     4021 gatcgtgctc gcgctcgcgt tcctcgtggg cggtctgtgg gtgtactttc ggcgctccga
     4081 cgtggtcggt acgctgttcg cattcaactc cgccatcctg gcttcgttcc tgaccgacgg
     4141 gccctcgacg ggaaaccccg cgctgcagct gtccgcggag atcgtgggcg acgcgatcat
     4201 cctcgcgttc cccgccacgt tgctccactt tttcttacgc tttcccgacc gcggcccgcg
     4261 cgagagcggt gcgcgccgcc gagcgtggct ctatattccg cccgccgtca tcttcgccgc
     4321 cagtgcggtg gtatcggcgc ggcgcttccg gttcgtcacc cacgaggtcg aggagcaggt
     4381 ggtcctcgcc ctctcgaccg cgtacttcgt ggcctacgtg ctctcgtcgc tggtggtgtt
     4441 cgtgcgcgcc taccgcgccg cgccgcgagc gcagaagcag aagctgcgcg tggtcatcgc
     4501 cggcaccgtc gcgggcctcc tgcccttcct ggccgccacc gtccacgcca gcattcgtcc
     4561 cgaagcgggt gccatgagct tcacgctccc cgccgagctg tgcctgggct tcgtgccgat
     4621 cgcgttcgcg tacgcgatct tgaagcacgg cgccatcgag ctcacgtcgg tggtgcgcaa
     4681 gagcctcgtc tacgcgctcc tcaccgggct cttgatcgca acctactacg cgctcgtgca
     4741 gaccgcgggt gcgtttctct cgcgcgagtt cggggtgagc gaggcggtct ggggcacgat
     4801 cgcggtcgtg gtgctcgcga tcggcttcgc gccggtgcgc gagcgcgtcc agggcgtggt
     4861 cgaccggatc ttctaccgcg ccgagtacgt ctacaagcag gaggtcatcg acttcggccg
     4921 gcaggtggcg cgcacgctga cccgcgagga gatgttcgac cacttcatcg cgcgctgcga
     4981 cgagctgttg cacccgacgt tcgtggtcgt gtatctgcgc ggtgaagaca aggacctcgg
     5041 tctcgagcgc gtgcacggtg cggcaccgga gatgaatccg acgttcaccg ccgactcgtt
     5101 cctggggcgc tacttcacgc gctaccgcac gccgctgttg gtcgagttcc tcgatcgctc
     5161 gtgggagcgt cccaacctgg acccggaggc gcggcgcctg ctctcgttgc cgggcctggc
     5221 ggcgtgcgtg cccatcgcgg cccccgatcg cctgctcggc gtagcactgc tcggccagaa
     5281 gcgctcgggc acggcctacc ggcgcgcgga cgccgagctc ttggagacct tcgcggacca
     5341 gctcgccctg gtggtcgaga acgccgattt gatccattcg tcgatcgaaa aggagcgcct
     5401 gaagagcgag gtcatgttgg cgcgcgacat ccagcaggcg ctcctgcccg cgaccgcgcc
     5461 ccggctgcgc gacgtcgaga tccgcggcca gatgatcagc tcgagcgagg tgggcggcga
     5521 ctacttcgac tatttcatgc tcgacgagcg ccgcatcgtg gtggcgatcg gcgacgtcat
     5581 gggcaagggg gttccggccg cgatgctgat ggcgtctctg caggcggtgt tcaagaaccg
     5641 cgcggccaag ggagggctcg cgccgcacga gctgaatcgc gagctcaacg actacctagt
     5701 cgagcacgcc aaacccggcc aatttgcgac cttcttctgt ggggttctcg acctggccca
     5761 ttcgacgttc accttctcga gcgcgggcca atgccccgcc ctcctggcca ccaatggttt
     5821 catcgaccga ctgggcaacg ggggcaccgt cctgggggcc aaccccaacc aggactacgc
     5881 cgagggcacg gtggccttcc accccggcga cctgctcatt ctgtacacgg acggggtgat
     5941 cgagcagatg aacgaccagg gggagcccta cggcgagcag cggttggtcg agttcgtgga
     6001 ggcgaatcga aacctaccgc ctgaccgact gcaaaacgca ctgttggccg acgtcctggc
     6061 attcgggggg gggcgccagg acgacgatat cacgagcgta attgcggtcc gtagaagcgc
     6121 ttagagcctc tttactcccc ttcccgccgt cccgtttctc accagggcac ggcctatgca
     6181 cgatcccccc cgacgccccg tcaggcaggc ggagcgccga tgagtt
//
LOCUS       DASPGK010000236         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_60783, whole genome shotgun sequence.
ACCESSION   DASPGK010000236 DASPGK010000000
VERSION     DASPGK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60783"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VEC56_06450"
     CDS             complement(<1..1525)
                     /locus_tag="VEC56_06450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXV13829.1"
                     /translation="MTARHRFALLCALLVSACGGPAQVPPSDTGRLDRLYKELADPLP
                     RFDPTLLSGFRILIDPGHGGTFRGTVGQDSLEESKVNLGVSLYLWGLLREAGADVHLT
                     RAIDRDFLTEVDSTLAFDLQKRVDVVDSLAPDLFVSIHHNAQPQRAPDYNRIETYYKA
                     GDPASLDLAFAIHRHLMRNLGIDVGEVRQGNYFVLRNVGVPAVLGESSYLTHPPVEDR
                     LELSRPQELEAEAYFLGILDYCRRGIPRVSAIGPVDSIQTDVPTVTCRLEDRGGLGVD
                     PDGVHMTVNGEAVDAILAADGRRASYPLPWDAPNGSYDVEVSARNLGGNTSPVAATTF
                     RIEHPPALAALESVPPRAPRAGGVVRVRARVLDRRGLPIADGTAVEMTASHAKGRERA
                     TVSGGAVEFSLDVPAGATKPVRVDLAAGGKSFRLDIPIEPARRSAWRTLAVTDATTGA
                     PITGAVVAAGDSVLAHASPTGAYGFEGGLAPVRVAAPGYRPADVAEGDTASLTPWFDG
                     "
     gene            1598..3010
                     /gene="selA"
                     /locus_tag="VEC56_06455"
     CDS             1598..3010
                     /gene="selA"
                     /locus_tag="VEC56_06455"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXV13830.1"
                     /translation="MAHPPESLRSIPSVDEVLAHPRLVAARAEHPRVPWTAIVRAALA
                     TLREELAFARAPADRAELTLRAVDEVRGRVAHLVSGGLKPVLNGTGVVLHTNLGRAPV
                     GRRAALAALAALEGYTSLEVDLESGERSKRGDVLEALLCWLTGAEAAMVVNNNAAAVV
                     LVANTFSPPGRVIVSRGELVEIGGSFRLPEILRHAAREVVEVGTTNRTYASDYEREAR
                     ACDVLLKVHKSNFEIEGFAHEATVAELVAVARARDAHAVFDLGSGALFDFAQRGLGND
                     PVASAVIETGVDAVTMSGDKLLGGVQAGIIVGRRAFVDRLKQNPLRRAMRVDKVAVAA
                     LQEVARAYLFAPSLSDEVPVIAAVSEGRERCRTRAERVAGCVAGAVDARWCVRAVNDD
                     AAVGGGSLASAVVPSSAVVVRGRDDAEAVRLARALRTRTLPVFVRVRGSEIRVNMTTI
                     FPAQEDSLIEALVEVLAARG"
     gene            3025..4440
                     /locus_tag="VEC56_06460"
     CDS             3025..4440
                     /locus_tag="VEC56_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV13831.1"
                     /translation="MNRVSIYQIPATSDVLSSAVSSPSVPPALVEPMQSMVAAIAHRA
                     TESPVRVYVCAEDALALARDGFATALARSMSAHVPSSVVVDCDFLRIGLSGLVPQPDA
                     LGLLDYLLYGSSIGVVTQEAAGGLRVVGAGSFPVTRRMPFVASAFDDAAQRLASHSRC
                     AIFVGPLLQEDGTAHPLASRANVTIVVRGDEGARAPRGGVNAIEEIIATDGVDVWSVR
                     AGAPEPKPARPVDTPRPASRPAAPAAARPKPAAAVAPEAARPRPRGAEALEPPESRYS
                     SLAPRIALLVFALFVIAFVAWWWWQGRSQPGAGEEAAPAAVDTPAETVPVPVHADTLA
                     VATVDSAAVSTPDSARASAPPATPPVRPEDTAGRTGGTVLVDPSDILVMADLESRWKD
                     WYAIHISSFQESIRAREEVSFLQSREFPVFIVFLDLGAKGKWYRVYAGPFRTREEARD
                     VKKNLDAIAQVRFTRITKIPE"
     gene            4489..>5452
                     /locus_tag="VEC56_06465"
     CDS             4489..>5452
                     /locus_tag="VEC56_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV13832.1"
                     /translation="MHLRKIEISGFKSFMSRIELDFSGGVTAIIGPNGCGKSNVVDSI
                     RWVLGEQRTRMLRNTKMENVLFNGTRLRKPLGMGEVFMTLDNEDHALDLEYKEVKIGR
                     RLYRSGVSEYLVNGQPVRLKDVRGLLVDTGLGNSAYAIIERDMIEKVLSEKEDEKRHL
                     LEEAAGVMRYRIQREEAERKIKATEQDLLRLVDILNELDKELRSLKYQMGKARRYAAL
                     KEQADAMESALLKAALFELLAKQSDLIAERESHENVRLVDDNEIAIAENRLQTLRVEA
                     AEFEKALQGLNEARFSTSQALQQHDERIAVHGERINANRTRIEED"
BASE COUNT          852 a         1859 c         1943 g          798 t
ORIGIN      
        1 tcccgtcgaa ccacggggtg agcgaggcgg tgtcgccctc ggcgacgtcg gcgggacgat
       61 acccgggtgc ggccacgcgc accggcgcca gtccgccctc gaagccgtag gcgccggtcg
      121 gcgacgcgtg cgcgagcacc gagtcaccgg cggccaccac cgcgccggtg atgggtgccc
      181 ccgtcgtcgc gtcggtgacg gcgagtgtcc gccacgccga gcgccgcgcc ggctcgatcg
      241 ggatgtcgag acgaaagctc tttccacccg cggcgaggtc gacgcgcacc ggcttcgtcg
      301 cgcccgcggg cacgtcgagc gagaactcga ccgcgccccc gctgacggtc gcgcgctcgc
      361 gccccttggc gtgcgacgcc gtcatctcca ccgcggtccc gtcggcgatc ggtaacccac
      421 gccggtcgag gacacgcgcg cgaacgcgca ccacaccgcc cgcgcgcggg gcccgcggcg
      481 ggaccgattc gagtgccgcc aacgcgggcg ggtgctcgat gcgaaacgtg gtcgcggcca
      541 ccgggctggt gttgccgccc aggttgcgcg ccgacacctc gacgtcgtag gagccgttgg
      601 gcgcgtccca cggcaagggg tagctcgcgc gccggccgtc cgcggccagg atggcgtcga
      661 ccgcttcgcc gttcaccgtc atgtgcacac cgtcggggtc cacacccaag ccgccgcggt
      721 cctccaggcg gcacgtcacc gtcggcacgt cggtctggat cgaatcgacc ggcccgatcg
      781 cgctcacgcg cgggatcccg cgccggcagt agtcgaggat ccccaggaag tacgcctcgg
      841 cctcgagctc ttgcgggcgg gagagctcca agcggtcctc gaccggcggg tgcgtgagat
      901 agctcgactc gcccagcacc gcgggcacgc ccacgttgcg caacacgaaa taattgccct
      961 gccgcacctc gcccacgtcg ataccgaggt tgcgcatcag gtggcgatgg atcgcgaagg
     1021 ccaggtcgag cgaagccggg tcgcccgctt tgtagtaggt ctcgatgcgg ttgtagtccg
     1081 gggcgcgctg gggctgcgcg ttgtgatgga tcgacacgaa caggtcgggt gcgagcgaat
     1141 cgaccacgtc gacgcgcttc tggaggtcga aggcgagcgt cgagtcgacc tcggtcagaa
     1201 agtcgcggtc gatcgcgcgc gtgaggtgca cgtcggcacc ggcctcgcgc aagagtcccc
     1261 acaaatagag cgacacgccg aggttgacct tcgactcctc taggctgtcc tgcccgacgg
     1321 tcccgcgaaa cgtcccgccg tggcccgggt cgatgagaat tcgaaagccc gagagcagcg
     1381 tcgggtcgaa gcgcggcagg ggatcggcca gttccttata gaggcgatcg aggcggccgg
     1441 tatccgaggg aggaacctgc gccggcccgc cgcacgcgga cacgagcagg gcgcagagca
     1501 gggcgaaccg gtggcgcgcg gtcatggtgg catgcctaac agaaacgtcc ccggccatca
     1561 aggatttgtt gcataatagg ttggctcgca atcacacatg gcgcacccac ccgaatccct
     1621 acggtcgatt ccatcggtcg acgaggtcct ggcgcacccg cggctggtgg ccgcgcgcgc
     1681 cgagcacccg cgcgttccat ggacggcgat cgtgcgcgcg gccctcgcga cgctgcgcga
     1741 ggagctggcg ttcgcgcgcg ccccggccga ccgcgccgag ctcaccctgc gcgcggtgga
     1801 cgaggtccgc gggcgcgtcg cgcacctcgt gtcgggcgga ctcaaaccgg tgctcaacgg
     1861 gaccggcgtg gtgctccata ccaatctggg ccgcgcgccc gtcggccggc gcgccgcact
     1921 cgcggcactc gccgcgctgg aagggtacac gagcctggaa gtcgacctgg aaagcggcga
     1981 acgctccaag cgcggcgacg tcctggaagc gctcctgtgc tggctcaccg gcgccgaggc
     2041 cgccatggtc gtcaacaaca acgcggccgc ggtggttttg gtggcgaaca cgttctcgcc
     2101 gcccgggcgc gtgatcgtct cgcgcggcga gctggtggag atcggcggga gcttccgact
     2161 tcccgagatc ctgcgccacg ccgcgcgcga ggtggtcgag gtcggcacca ccaatcgcac
     2221 ctacgcgtcc gactacgaac gcgaggcgcg cgcgtgcgat gtgctgctca aggtgcacaa
     2281 gagcaacttc gagatcgaag gattcgccca cgaggcgacg gtggccgagc tggtggcggt
     2341 ggcgcgcgcg cgcgacgccc acgcggtgtt cgacttgggc agcggggcgc tcttcgactt
     2401 cgcgcagcgc gggctcggca acgacccggt ggcgagtgcg gtgatcgaaa ccggggtcga
     2461 cgccgtcacc atgagcggcg acaagctatt ggggggcgtg caggcgggga tcatcgtggg
     2521 gcggcgcgcg ttcgtcgatc gcctcaagca aaatccgttg cggcgcgcga tgcgcgttga
     2581 taaagtggcc gtcgccgcgc tccaagaagt cgcgcgcgcc tatttgttcg cgccgagtct
     2641 atccgacgaa gtaccggtca tcgccgcggt gagcgaaggg cgcgagcgct gccggacccg
     2701 cgccgagcgc gtggctgggt gtgtggcggg ggcggtcgat gcgcgctggt gcgtacgcgc
     2761 cgtgaacgac gacgcggcgg tgggcggcgg gagcctcgcg agcgcggtgg tgccgtcgag
     2821 tgcggtggtg gtgcgcggtc gcgacgacgc ggaggcggtg cggctcgcgc gcgcgttgcg
     2881 cacgcggacg ctcccggtgt tcgtgcgcgt gcgcggcagc gagatccggg tcaacatgac
     2941 gaccattttt cccgcccagg aagattcact gatcgaggcg ttggtcgagg tgctggccgc
     3001 gcgcggctga tcgtcagaga catcatgaat cgcgtttcca tctaccagat tccggccacc
     3061 tccgacgtcc tctcgtcggc agtctcgtcc ccgtccgtcc cgcccgccct ggtcgagccc
     3121 atgcagtcga tggtggcggc gatcgcgcac cgtgccaccg agtcgccggt acgcgtgtac
     3181 gtgtgcgcgg aagatgcgct ggcgctggct cgcgacggct tcgcgaccgc gctcgcgcgt
     3241 tcgatgtcgg cgcacgtgcc gtcgtccgtc gtagtggatt gcgatttcct ccgcatcggg
     3301 ctctcgggcc tggtcccgca acccgacgcg ctggggctct tggactactt gctgtacggg
     3361 agctcgatcg gtgtggtgac gcaggaggcc gcgggcggac tgcgcgtggt gggcgcgggg
     3421 tcgttcccgg tgacgcggcg catgcccttc gtcgcatccg ccttcgacga cgccgcgcag
     3481 cgcctcgcgt cgcactcacg ctgcgccatc ttcgtgggtc cgttgctgca ggaagacggg
     3541 accgcgcacc cgctggcatc gcgcgcgaac gtgaccatcg tagtgcgcgg cgacgagggc
     3601 gcgcgcgcgc cgcgcggtgg cgtcaacgcc atcgaggaga tcatcgcgac cgacggcgtc
     3661 gatgtctgga gcgtgcgcgc gggcgcgccg gagccgaaac cggcgcgccc ggtcgacaca
     3721 ccgcgcccgg cgtcgcggcc ggcggcaccc gcggccgctc gcccgaagcc ggccgccgcc
     3781 gtcgcccccg aggccgcacg accgcggccg cgcggcgcgg aggccctgga gccgcccgag
     3841 tcgcgctaca gctcgctcgc gccgaggatc gccctgctcg tgtttgcgct gttcgtcatc
     3901 gccttcgtcg cgtggtggtg gtggcagggg cgttcgcaac cgggagccgg ggaagaagcg
     3961 gcgccggccg ccgtcgacac gcctgccgag acggtcccgg tgccggttca cgccgacacg
     4021 ctcgcggtcg ccaccgtcga ttccgcggcc gtgtcgacgc ccgatagcgc gcgcgcgtct
     4081 gcgccgccgg ccacgccgcc ggtccgtccc gaggacacgg ccgggcgcac gggcgggacc
     4141 gtgctcgtcg acccgtccga catcctggtc atggccgacc tcgagagccg atggaaggac
     4201 tggtacgcga tccacatctc gtccttccag gaatcgatcc gtgcgcgcga agaagtctcc
     4261 ttcctccaga gccgcgaatt cccggtcttc atcgtgttcc tcgacctcgg cgccaagggc
     4321 aagtggtacc gcgtgtacgc gggtcccttc cggacgcgcg aggaggcgcg ggacgtcaaa
     4381 aaaaacctgg acgcgattgc gcaggtacga tttactcgca tcacgaagat tccggagtga
     4441 cggtttcgcc gcgtggcgac gcccagttcg ctccgcggag gacctttttt gcacctgcgt
     4501 aagatcgaaa tctcgggctt caagtccttc atgtcgcgca tcgagctcga cttctcgggc
     4561 ggggtcacgg ccatcatcgg ccccaacggg tgcggcaagt ccaacgtggt cgactccatc
     4621 cgctgggtgt tgggcgagca gcgcacgcgc atgctgcgca acacgaagat ggaaaacgtc
     4681 ctgttcaacg ggacgcggct ccgcaagccc ctggggatgg gcgaggtctt catgaccctc
     4741 gacaacgagg accacgccct cgacctcgag tacaaggaag tcaagatcgg ccgccgcctc
     4801 taccggagcg gggtcagcga gtacctggtg aacggccagc ccgtgcgcct caaggacgtg
     4861 cgcggcctgt tggtcgacac cggccttggc aacagcgcgt acgccatcat cgagcgcgac
     4921 atgatcgaga aggtgctctc ggagaaagag gacgagaagc gccacctgct cgaagaggcg
     4981 gccggtgtga tgcgctaccg catccagcgc gaggaagccg agcgcaagat caaggctacc
     5041 gaacaggacc tcctgcgcct ggtcgatatc ttgaacgaac tcgacaagga actgcgctcg
     5101 ctcaagtatc agatgggtaa ggcgcggcgg tacgccgcgc tcaaagagca ggccgacgcg
     5161 atggagagcg cgctcctgaa ggcggcgctg ttcgaactgc tcgcgaagca atcggatctc
     5221 atcgccgagc gcgagtcgca cgagaacgtg cgcctagtcg acgacaacga gatcgcgatc
     5281 gccgagaatc gcctgcaaac cctgcgggtg gaagcggccg agttcgagaa ggcgctgcaa
     5341 ggcctgaacg aggcgcgctt ctccaccagc caggcgctcc agcagcacga cgagcgcatc
     5401 gcggtgcacg gcgagcgcat caacgcgaac cgcacccgaa tcgaagaaga cg
//
LOCUS       DASPGK010000237         8554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_60863, whole genome shotgun sequence.
ACCESSION   DASPGK010000237 DASPGK010000000
VERSION     DASPGK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60863"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2455)
                     /locus_tag="VEC56_06470"
     CDS             complement(<1..2455)
                     /locus_tag="VEC56_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13833.1"
                     /translation="MNRIFRRSTLVALCLVCLSAGETLAASNQYVESFNTTTYKDAAN
                     TTALWDVGAGELKLAPFFPSLVGAYDTPDFSYDVAVAGDLAFVADYDLGLQIISIANP
                     GSPSLVGNYNTPGAAFGVAVSGDLAFVADMASGLQIINITNPASPALVGSYDTPGVAL
                     GVAVDGDLAFVADHSGGLRIINIANPASPSLVSTYNTPRPPYYVAVAGDLAFVAAEGS
                     GLQIINITNPASPALVGTYDTPGLALGVAVDGDLAFVADHSGGLQIIDITNPASPFLA
                     GTYLTGWLAYGVAVSGDLAFVADHTNGLQIVDISNPGSPFLAGTYDTPDTAYRVAVDG
                     ELAFVADYSSGLQVVKVTTRARPRLVGAYNTPGNAASVAVAGNLAFVADGASGLQIIN
                     ITNPASPSLFGTYNTPGTAGRVVVSGDLAFVADDDFGLEIINITNPASPSLVGMYNAP
                     SEVADVAVAGDIALVAINFFGLQIVNISNPASPSLVSTYDTPGFATRVAVTGDLALVA
                     DFGSGLQFINISNPASPSLVSTFDTPGTMYDIVVSGNLAFVADGESGLTVVSIANSAS
                     PSLVGTFDTSEQAVGVAVTGDLAFVNDIVSGLHVIDIANPASPSLVGTYATSEQVGGV
                     VVAGSLAFVADGPAGLSGIEVFNHRFNTLDHIGRSLKVNATADPVIAVRLSTTQTPSV
                     TWEISADGGAAWQGITPDNAWNLVAAEANDLRWRSTHTLSGWPASNPIGSDLQIEWLY
                     EFALIDAITDVPNDQGGWVRLKLVRSGRDFAAETSHPIENYGVWRRIDNAAVLAEIAS
                     TRRGAEPIDASIVEKFGALP"
     gene            2572..5886
                     /locus_tag="VEC56_06475"
     CDS             2572..5886
                     /locus_tag="VEC56_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV13834.1"
                     /translation="MTGREDTARWARIAEIFGKALAAADREGVVERECAGDPALAGEV
                     RSLLAHHDGADGFLDPRAMQLKGIDESLAEDLEAELGSDASDALTGTSVGAWRVIEPI
                     ARGGMGTVYRVQRAEGGFEQTGALKVIRRGFATDESVRRFRRERKILAGLEHANIAHL
                     LDGGTTPDGLPYLVLEHVVGTSLLDYCNAKQAGVEERIRLFLDVCAAVQSAHQKLVLH
                     RDLKPSNILVTPDGTVKLLDFGVAKIFAEESDTDPSALEPRTVHLPMTPGYASPEQVR
                     GDDATIASDVFSLGVVLYELLTGRRPYAPTATQSTASMLRSFERQPARPSDAVEIPSQ
                     KKRLRGDLDTIVLRAVQVDAVRRYPSVEQLAGDIERFLAGKPVLARPDRWSYRASKFI
                     RRNRVAAAGAAAGVIALIAGLVTALWQVQVARHERAVAERRFDEVRSIAGTLMFDLHD
                     ALLTVPGTATMRELVLANAGRYFDTLFEDAAEDQSLALDVAEAYERLGRVQSTTSAAR
                     AREAAHASLRRSLGLRETVLKTDAHNDRARLGVVTACAALVDLEHSSGRVAEATAWAR
                     RALAAAEDAPATEPGRAGAAVAASVTNTAAPLPHAAQARASTVLAATNASDWVCIDRE
                     PAPGWNTDAAFDDSRWIAPTEGSGYAEYAPHGAPIWARSVGCGGSAPSDVWFRHTWEM
                     NEPLPNVALLRVHVDDDASVWLNGHPVVVDQDSCFAPDVIPFDVRPYLRAGKNLLAVR
                     AHDCQSVCRALYAEIEASERLDVFAATNAQQWTCTDEVPPPGWNFDIAFDDRAWRAPA
                     VQERPTGLEQAQGIWADSVGCFGAVGPSQDVWFRSVFTLREQPGAAFMQGAVDDDWTI
                     WVNGLEVWFDRDCESRLLPFMDITPYLHVGDNLIAVRAHDCDYCRSMCLEIVATPPAP
                     IPASVPGDGYVGVYEDSAGTAPCVTIPAGQLKVLYVVAHLAGRTAAGFRAAEFRIEFT
                     NPTSYAAVWVEDDAVRCEGNPLDLTPRVVDATGARVSLSNAVPGARQVTLGRIVVGNA
                     GGPPCRLLVMANFAPSRSTIDGPVFLHDGRSGTELVPMSAARHGFAGWPVAFVGAIND
                     PACAGVASDHASARSH"
     gene            5802..6464
                     /locus_tag="VEC56_06480"
     CDS             5802..6464
                     /locus_tag="VEC56_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV13835.1"
                     /translation="MAGRLRRRHQRPSLRGCSERPRVGAVALGGSPITQPRDITATLI
                     RWQQGDRTALDVLTPLVYDQLRSLAASYLSRERVGHTLQPTALVNEAFLRLVDQTQVT
                     WQNRAHFLGIAAQAMRRILVDHARRKRAAKRGEGAIHVSFEMLGSVSGPSRDVALDDL
                     DAALDDLARLEPRQARVVELRFFGGLSIEEAAEVMGTSPATTKRDWTVARAFLHRALI
                     GD"
     gene            complement(6494..>8554)
                     /locus_tag="VEC56_06485"
     CDS             complement(6494..>8554)
                     /locus_tag="VEC56_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13836.1"
                     /translation="LRNGNYDIYAQRVNGAGVPQWASGGVAICTDGGTQRFPRIVSDA
                     AGGAIIAWSDNRYFTSDFFAQRINGSGVGQWGVNGVPMFVDFYLQDFLAMAPDQDGGA
                     ILAWIDYQFADQRIMAQQVTSAGALYYPVGVGLVVCSAPGDKGEIVIARDGAYGATVA
                     WTDTRGGTDLHLYAQRITGSGALWWQPDGIPISTVSGTEDLAIAFDGAGGAVVSWSDY
                     RNGFDADVYAQRFNEQGLTYWPANGALVCSAPSHQWETSVIPDGAGGAIVSWLDLRAM
                     PFYADIYANRVNSSGAVTWAFNGIPLCTAIANQRRPIVVTDGDGGAIVAWEDFRSGNS
                     DLHAQRVEKRGYWGRPEPFAESASDVPGDQGGKVAVNWTASGQDIYDQQIVTHYSVWR
                     ATDVEPAAAAIVSSAEEIEPDFQGTAYRRELDAAGDEYYWEWVGNQSAIYSPRYSFSA
                     STRSDSTSQGNADHFFQVLAHTWNNFVHWTSNVVSGHSLDNLAPAAPLLLTAQRVGRD
                     VHLRWNRSIAPDVRDYSIYRATTTGVTPVPANFMASATDTVLVDESTPVSALYYIVTA
                     YDVHENQSDASNEANVGALTGSGETPSVRALTVLQNYPNPFTGSTELEIGLPASADVS
                     IQVYDVAGRVVREERLAGRPAGWQRIALDGRGRDGKPLASGVYFYRVTAAGQTVTKKM
                     VIAR"
BASE COUNT         1459 a         2800 c         2828 g         1467 t
ORIGIN      
        1 tcggcaacgc gccgaatttc tccacgatcg aggcatcaat gggttcggca ccgcgccggg
       61 tggatgcgat ctccgcgagt actgccgcgt tatcgatgcg ccgccacacg ccgtagttct
      121 ctattggatg cgacgtctcc gccgcgaaat cccgtcccga gcgaacgagc tttaggcgca
      181 cccaaccccc ttgatcattg gggacgtcgg tgatcgcgtc gatcagggcg aactcgtaca
      241 gccactcgat ctggagatcc gagccgatcg gattcgaggc gggccacccg ctgagcgtgt
      301 gcgtcgagcg ccagcgcagg tcgttggcct cggcggccac caagttccac gcattgtccg
      361 gtgtgatgcc ctgccacgcc gcgccaccgt cggcgctgat ctcccaggtg acgctgggcg
      421 tctgagtcgt cgagagtcgc accgcaatca cggggtccgc cgttgcattg accttgaggg
      481 agcggccgat gtggtccaat gtattgaatc gatgattgaa cacctcgatt ccggagaggc
      541 cagcaggccc gtccgccacg aacgcgaggc tgcccgccac cacaacgccc cccacttgct
      601 ctgacgtggc gtacgtgccc acgagcgagg ggctcgccgg attggcaata tcgatgacgt
      661 ggaggccgga gactatgtcg ttcacgaagg cgaggtcgcc cgtcaccgca actccaaccg
      721 cctgttccga cgtgtcgaac gtaccgacga gcgaggggct cgccgaattg gcaatactga
      781 cgaccgtgag gccggactct ccgtccgcca cgaaggccag gttccccgat acaacgatgt
      841 catacatagt ccccggtgtg tcgaacgtgc tgacgagaga cgggcttgcc ggattggaga
      901 tgttgatgaa ctgaaggcca gagccgaaat cggccactaa ggcgaggtcg cccgtcaccg
      961 caacgcgagt cgcaaagccc ggagtgtcgt acgtgctgac cagcgagggg ctcgccggat
     1021 tggagatgtt aacgatctga aggccgaaaa agttgatagc cacgagggcg atgtcgcccg
     1081 ccaccgcaac atcagccact tcgctcggcg cgttgtacat gcccaccagc gaggggctcg
     1141 ccgggttcgt gatattgata atctcaaggc cgaaatcatc gtcggccacg aaggcgagat
     1201 cccccgatac cacgacgcga cccgcagtgc ccggcgtgtt gtacgtgccg aacagcgagg
     1261 ggctcgccgg gttcgtgata ttgatgatct gtaggccgga cgctccgtcc gccacgaagg
     1321 cgagattccc cgccaccgca acgctagccg cgttgcctgg cgtattgtac gcgccgacga
     1381 gccgcggcct cgcgcgagtg gtgactttga cgacctggag tccggacgaa tagtccgcca
     1441 cgaaggccag ttcgccgtct accgcaactc gatacgcagt gtccggcgtg tcgtacgtgc
     1501 cggcgagaaa ggggcttccc gggttgctga tgtcgacgat ctggaggcca ttggtgtggt
     1561 cggccacgaa ggccaagtcg cccgataccg cgacgccgta cgcaagccac cctgtcaggt
     1621 acgtgcccgc gaggaacggg ctcgccggat tggtaatgtc gatgatctgc aggccgcccg
     1681 agtggtccgc cacgaaggcg aggtcaccgt ctaccgctac tccgagcgca agccccggcg
     1741 tatcgtacgt gccgacgagg gcggggcttg ccggattggt gatattgatg atctggaggc
     1801 cggacccttc ggccgccacg aaggcgaggt cgcccgctac cgctacgtag tacggaggtc
     1861 tcggcgtgtt gtacgtgctg acgagggagg ggcttgccgg attggcaatg ttgatgatcc
     1921 gcaggccgcc cgagtggtcc gccacgaaag cgaggtcacc gtctaccgcg acgcccagcg
     1981 caacgcccgg cgtgtcgtac gaaccgacga gggcggggct tgccggattg gtgatattga
     2041 tgatctggag gccggatgcc atgtccgcca cgaacgcgag gtcccccgat acagcaacgc
     2101 caaacgcagc ccccggcgtg ttgtaattac cgacgaggga cgggctgccc gggttggcga
     2161 tgctgatgat ctggaggccg agatcatagt ccgccacgaa ggcgagatcc cccgccaccg
     2221 ccacatcgta agaaaagtcc ggcgtatcgt aagcgccgac gagggaggga aaaaacggcg
     2281 ctagcttcaa ttcaccggca ccgacgtccc agagggccgt ggtgttggcg gcgtccttgt
     2341 acgtcgtcgt gttgaaactc tcgacgtact ggttcgacgc ggcgagggtt tcacccgcgg
     2401 atagacaaac gaggcacagg gcgaccaagg tggatcggcg gaatatgcgg ttcatagcta
     2461 tacctctgcg tggttgatgt ttccacgccc tcttccaacg cgcaaaacgg gctcaaatcg
     2521 gctcacgccg ggacgtattc aagggacgac gggtggtata atggacgtcc catgaccggc
     2581 cgagaggaca ccgcccgctg ggcgcggatc gccgaaatct tcggtaaggc actggccgcc
     2641 gcggatcggg agggagttgt cgagcgcgag tgtgcggggg atcccgccct cgccggcgag
     2701 gtgcgctcgc tcttggccca ccacgacggg gccgacggct tcctcgaccc cagggcgatg
     2761 cagctcaagg gaatcgacga gtccctggcc gaggacctcg aggctgagct cggatcggac
     2821 gcctcggacg ccctcacggg gacgagtgtc ggggcgtggc gggtgatcga gccgatcgcg
     2881 cgcggcggaa tggggacggt gtaccgggtc caacgcgcgg agggcggatt cgagcagacc
     2941 ggcgccctca aggtgatccg gcgcggcttc gcgaccgatg aatcggttcg gcgcttccgc
     3001 cgcgagcgaa agatcctcgc cggattggag cacgcgaaca ttgcccacct cttggacggc
     3061 gggaccacac ccgacggtct cccctacctc gtcctcgagc acgtcgtcgg cacgtcgctc
     3121 ctcgattact gcaacgccaa gcaagcgggc gtcgaagagc gcatacggtt gttcctcgac
     3181 gtgtgcgccg ccgtccagtc cgcgcaccag aagctcgtac tccaccgcga cctgaagccg
     3241 agcaacatcc tcgtcactcc tgacgggaca gtgaagctcc tcgatttcgg agtggcgaag
     3301 atcttcgccg aggagagcga caccgatcca tccgcgctcg agccgcgcac ggtgcatctg
     3361 cccatgacgc cggggtatgc gagtcccgag caggtgcgcg gcgatgacgc aaccatcgcg
     3421 agcgacgtct tctcgctggg tgttgtgctc tacgagctgt tgaccggccg ccgaccgtac
     3481 gcgccgacgg cgacgcaatc gacggcgtcg atgctgcgca gcttcgagcg gcagccggca
     3541 cgaccgagcg acgccgttga aattccttcg cagaagaaaa ggctccgcgg ggatttggat
     3601 accatcgtac tgcgcgcggt gcaagtggac gcggtccgtc gctacccgtc cgtcgaacag
     3661 ctcgccggcg acatcgagcg cttcctcgcc gggaaaccgg ttcttgcccg tcccgatcga
     3721 tggagttatc gcgcatccaa attcatccgc cgaaatcgcg tcgccgccgc gggcgcggcc
     3781 gcgggtgtca tcgccttgat cgccggcctc gttactgcgc tctggcaggt tcaggtcgcg
     3841 cgtcacgagc gagcggtcgc cgagcgtcgc ttcgacgaag tgcgatccat cgcaggaacg
     3901 ctcatgttcg atctgcacga cgcgttgctg accgtgccgg ggacggcgac gatgcgcgag
     3961 ttggtgctcg ccaacgcggg acgctacttc gacacgctct tcgaggacgc ggccgaggat
     4021 cagtctctgg cgttggacgt cgccgaggcg tacgagcggc tgggtcgggt gcagtcgacg
     4081 acgtcggcgg cgcgcgcgcg cgaggccgcg cacgcgagtc tccggcgctc gttggggctt
     4141 cgcgaaacgg tgctgaagac ggacgcccac aacgaccgcg cgcggctcgg cgtggtgacc
     4201 gcgtgcgccg cgctggtcga tctggaacat tcgagcgggc gagtcgccga ggcaaccgcg
     4261 tgggcgcggc gcgccctggc cgctgcagaa gacgcgccgg cgaccgaacc gggacgcgcg
     4321 ggagcggcgg tggctgcgtc tgtgacgaac acggcggcgc ctttgccgca tgcagcgcaa
     4381 gcgcgtgcat cgaccgtcct ggcggcgacg aacgcgtcgg actgggtgtg catcgatcga
     4441 gagccggcgc ccggttggaa caccgatgcg gctttcgacg attcccgttg gatagcgccg
     4501 accgagggat cgggctacgc ggagtacgcg ccgcacgggg cgcccatttg ggcgcgctcc
     4561 gtggggtgcg gcgggtccgc tccttccgac gtgtggtttc gacatacctg ggagatgaac
     4621 gagccgctcc cgaacgtggc actgcttcgc gttcacgtcg acgacgacgc ctcggtatgg
     4681 ctcaacggtc accccgtcgt ggtcgaccag gactcatgct tcgcgcccga cgtgattcct
     4741 ttcgacgtga ggccttacct gcgcgccgga aaaaacctgc tcgccgtacg cgcccacgac
     4801 tgccagagtg tttgtcgcgc gctgtacgcg gagatcgaag cgagcgagcg tctcgacgtt
     4861 ttcgcggcca ccaacgcgca gcagtggacg tgcacggacg aggtcccgcc gccgggatgg
     4921 aacttcgaca tcgccttcga cgaccgagcg tggcgtgcgc cggcggtgca ggaacggccg
     4981 acgggactcg agcaagcgca aggcatctgg gcggactcgg tcggctgctt cggggccgtc
     5041 ggtccgtcgc aagacgtgtg gttccgctcg gtgttcacgc tgcgcgagca accgggcgcc
     5101 gcgttcatgc agggtgcggt cgacgacgat tggacgatct gggtgaacgg tctggaagtc
     5161 tggttcgacc gcgactgcga atcgaggctg ctgccgttca tggacatcac gccttatctc
     5221 cacgtcggcg acaatctcat cgcggtccgc gctcatgact gcgactactg ccgaagcatg
     5281 tgcctcgaga tcgtcgccac gccccccgca ccgattcccg cttccgtgcc gggcgacggg
     5341 tacgtcggcg tctacgaaga ctcggcggga acggcgccgt gtgtaacgat tccggcggga
     5401 caactgaagg tgctgtacgt cgtggcccat ctcgccggac gcacggcggc gggattccgc
     5461 gccgccgagt tccgcatcga gttcaccaac cccacgagct acgccgctgt atgggtagag
     5521 gacgacgccg tgcgctgcga aggaaacccg ctcgacctca ctccccgggt tgtcgacgca
     5581 accggcgcac gtgtttcact gtccaacgcg gtgcccggcg ctcggcaggt gacactcggc
     5641 cggatcgtgg tgggaaacgc gggtggcccg ccttgccggc tgctggtcat ggccaacttc
     5701 gctccgtcgc ggtccacgat cgacggcccc gtgttcctgc acgacggtcg gagcggcacg
     5761 gagttggtgc ccatgtcggc ggcgcgtcac ggcttcgccg ggtggccggt cgccttcgtc
     5821 ggcgccatca acgacccagc ttgcgcgggt gtagcgagcg accacgcgtc ggcgcggtcg
     5881 cactaggagg ttcccccatt acccaacccc gcgacatcac cgcgacactc atacgctggc
     5941 agcaggggga tcgcacggcg ctcgacgtcc tcacgccgct cgtctacgac cagttgcgct
     6001 cgctggctgc gagctatttg agccgcgagc gcgtcggcca caccctgcag ccgacggcgc
     6061 tcgtgaacga agcgttcctg cgcctcgtcg atcagaccca ggtgacttgg cagaaccgcg
     6121 cccatttcct cggcatcgcc gcccaggcga tgcggcgtat tctggtcgac cacgcgcgga
     6181 gaaagcgcgc cgccaagcgc ggcgaggggg cgatccacgt gtcgttcgag atgctcggat
     6241 ccgtctccgg cccttcgcgc gacgtcgccc tcgacgatct cgacgccgcc ctcgacgacc
     6301 tcgcccgact cgagccccgc caggcgcgcg tggtcgagct gcgcttcttc ggggggctct
     6361 cgatcgaaga ggccgccgag gtgatgggga cgtcaccggc gacgacgaag cgcgactgga
     6421 cggtcgcgcg cgccttcctg catcgggcgt tgattgggga ctagcgcgcg ctcgcgatcc
     6481 tcgtgggcct ttactagcgc gcaatcacca ttttcttggt cacggtctgg cccgccgctg
     6541 tgacgcggta gaagtacaca ccgctcgcga gcggcttgcc gtcccggcct cgtccgtcca
     6601 gcgcgatgcg ttgccacccc gccggtcgtc ccgccagccg ctcttcacgc acgacgcgcc
     6661 ccgcgacgtc gtagacctga atcgacacgt ccgcgctggc cggcaacccg atctcgagtt
     6721 ccgtggaacc ggtgaagggg ttggggtaat tctgcaggac ggtgagcgca cggactgacg
     6781 gcgtttcgcc gctgcccgtc aacgcaccca cgttggcctc gtttgacgca tcactctggt
     6841 tctcgtgcac gtcgtacgcc gtgacaatgt agtagagcgc cgagaccggc gtgctctcgt
     6901 cgacgagcac ggtatccgtg gccgaggcca taaagttggc cggcaccggc gtcactcccg
     6961 tcgtggtcgc gcgatagatc gagtagtcgc gcacgtccgg agcgatactt cgattccagc
     7021 gcaaatgcac gtcgcgaccc acgcgttgcg cggtcaatag caacggtgcc gccggcgcga
     7081 ggttgtcgag ggaatgaccg ctaacgacgt tggaggtcca atgcacgaag ttgttccagg
     7141 tgtgcgcaag gacttgaaaa aagtgatccg cgtttccctg gctggtcgaa tcgctgcgcg
     7201 tggaggcact gaaggagtag cgcggtgaat agatggcgga ctggttcccc acccattccc
     7261 agtagtactc gtcgccggcc gcgtcaagct cgcggcgata ggcggtaccc tgaaagtcgg
     7321 gctcgatctc ctctgcgctc gaaacgatcg ccgcggccgc cggctcgacg tcggtagcgc
     7381 gccagacgga gtaatgggtc acgatttgct gatcgtagat gtcctgcccg ctcgctgtcc
     7441 aattgacggc gacctttcct ccttggtcgc cgggcacgtc gctcgcggac tcggcgaacg
     7501 gctccggccg tccccagtac ccgcgcttct cgacgcgttg cgcgtggagg tcgctgttgc
     7561 cggatcggaa gtcctcccac gcgacgatcg cgccgccatc gccgtccgtt acgacaatcg
     7621 gccggcgctg attggcgatg gctgtgcaca acgggatccc gttgaacgcc cacgtgaccg
     7681 ctcccgagct gtttacccga ttcgcgtaga tatctgcgta gaacggcatg gcccgcaagt
     7741 ccaaccacga gacgatggcc ccgccggcac cgtcgggtat cacgctggtt tcccactgat
     7801 gggacggcgc cgagcacacg agcgcgccat tcgccggcca gtatgtcaac ccttgttcgt
     7861 tgaaacgttg tgcgtacacg tcggcgtcga agccgttgcg gtagtcgctc cacgacacga
     7921 cggcaccacc ggccccgtca aaagcgattg ccaggtcttc cgtgccgctc acggtcgaga
     7981 tcgggatacc atccggctgc caccataggg ctcccgagcc ggtgattcgc tgggcataca
     8041 ggtgcaggtc cgtgccgcct cgagtgtcgg tccacgcgac ggttgctccg tatgccccgt
     8101 cccttgcgat gacgatctcg cccttgtcac ccggtgccga gcaaactacc agcccaactc
     8161 caaccggata gtagagggcg ccggctgaag tgacctgttg ggccatgatc ctctggtcgg
     8221 cgaactgata gtcgatccac gcgagtatcg cgcctccgtc ctgatccgga gccatggcga
     8281 gaaagtcttg gaggtagaag tcgacgaaca tcggcacgcc gttgaccccc cattggccga
     8341 cgccggaacc gttgatgcgc tgcgcgaaga agtcggaggt gaagtatctg ttgtccgacc
     8401 atgcaatgat cgcaccgccc gcagcgtccg acacgattct cggaaaccgc tgcgtgcccc
     8461 cgtcggtgca gatcgcaacg ccccccgatg cccactgggg cacgccggct ccgttcacgc
     8521 gctgcgcgta gatgtcgtag tttccgtttc gcag
//
LOCUS       DASPGK010000238         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_646772, whole genome shotgun sequence.
ACCESSION   DASPGK010000238 DASPGK010000000
VERSION     DASPGK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646772"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VEC56_06490"
     CDS             complement(<1..742)
                     /locus_tag="VEC56_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HXV13837.1"
                     /translation="MTTAVRSFLDAHVARIEPLSRDVNLAYWSATISGKPEDFERYAR
                     LEVELQKVYTSAREFEEVKGWLAASGVDGLERRQLDLLYRVYLRNQIDPAHIEEMTRL
                     SSKIVQSFGTYRAHVDGRSLTGNDVRNALKQSTDAAYRRRVWEADKAVGREVRDDLLR
                     LVRLRNEVARSLGFADYYVMSLELSEQQADDMSALFDRLDLATREPFARAKAEIDAAL
                     ALRYAVRPGDLRPWHYEDPFFQEAPRVFD"
     gene            944..1573
                     /locus_tag="VEC56_06495"
     CDS             944..1573
                     /locus_tag="VEC56_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV13838.1"
                     /translation="MEASLRAAAGPALPELPLNVENEREIIRGWKRGDKRAYEALVRR
                     YMTDAYLVAYGYVGNAEDARDLSQEAFVKVYQARARFDEERPFYPWLYRVLRNHCLNF
                     LRRGRVEVSIDDENAHREIASPRPTVLDDMESDERRRIVRSAVARLSDDHREIIVLKN
                     FKDHSYKEIAEILEIPIGTVMSRLFYARQALRALVEEIEGRGPIKEEAF"
     gene            1570..2010
                     /locus_tag="VEC56_06500"
     CDS             1570..2010
                     /locus_tag="VEC56_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV13839.1"
                     /translation="MTPACTRYQSDGMRWLDGELTAQEADHYQAHVRECDECRRELAS
                     LGRVVRMTDELKLRAPDDEFWKGYWESVYRRSERRVGFVLLIAGVLAVLGYAIFRAVT
                     SPELLTYEGISVMLILGGLVVIFISVARERYHEHKSDPYKEVER"
     gene            2007..2327
                     /locus_tag="VEC56_06505"
     CDS             2007..2327
                     /locus_tag="VEC56_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HXV13840.1"
                     /translation="MIVVTTDDVPGHRVTQVFGLVQGSSVRARHVGKDILASFKNLVG
                     GEIHEYTKLLAESREQALDRMREEAARMGANAIVGMRFSSTDISQGASELLVYGTAVQ
                     IERA"
     gene            2374..3216
                     /locus_tag="VEC56_06510"
     CDS             2374..3216
                     /locus_tag="VEC56_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV13841.1"
                     /translation="MRFGDWNVRTCIAGRFGLDGGAMFGVVPKTMWERLLPPDSYNRV
                     PMVMRTLLVQGHGKTVVVDVGAGGGHSEKNRQIYAFEDTDHLTDAIRAHGVEPAEVTD
                     VLLTHLHFDHGAGIVERDGDDWRLVFPRAVHHLQTSQWKHALSPNPRDRASYYDDRIR
                     VLEREKVLRLYDGPWSLSPGFDLLVFDGHTPGQQLPVIGGGVGSLFFCGDLFPTHHHI
                     PTPYVMSYDLYPVTAMEEKAALLERAEAGKWLLCFEHDAHMEACRVTREGKRFTAGET
                     VRIL"
     gene            3344..4132
                     /locus_tag="VEC56_06515"
     CDS             3344..4132
                     /locus_tag="VEC56_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13842.1"
                     /translation="MTRKIMTLGTVAAFGVAAALVGAGDAGLAFACDKATKTAAATTS
                     SSDCSKDATKASATTASVKTASASCCAKDAKTTTAGNVHKSMIKSAVVAPGAAPVLNT
                     AAFAAGVSGSTVAAGSGSCCESKSASAASAAGACDKSAAVKTAGAGGSCDKSAAAKTA
                     GASGSCEKSTKTAAAAVDQVPYRESKRVVLTGTYECGHCGIGATAECAPMFKTADGKV
                     YPLWNSDRVSSLRNDKAAKNVEIATLVKKVDGVKYLDVKSYKTL"
     gene            complement(4235..>4987)
                     /locus_tag="VEC56_06520"
     CDS             complement(4235..>4987)
                     /locus_tag="VEC56_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13843.1"
                     /translation="GPYYPYYGPGWSFSIGWGWGWGWWGGYYCSGWYAPCGWGYGYDG
                     YYGSGSYAYPEKFKAKYKSESASTLTGTRAVAEKRDGSLRIASKDVQQSLSRPAQSSV
                     RSKTGTATRTTFGSGKSRGAIDASPRVKTSIGGRSSGQAKWGTTRGGAARGSATTPRV
                     RSTGSSRKGVADVQRGGGGYKSRSVAPGTRGSSRPSGGRVGSSPSSAPRAKSTPSRSY
                     SAPSSRGGSMKGSAPRSAPSGGSRGGSRGRGK"
BASE COUNT          855 a         1600 c         1695 g          837 t
ORIGIN      
        1 cgtcgaagac gcgcggcgct tcctgaaaaa acggatcctc gtagtgccaa gggcgcaagt
       61 cccccggccg caccgcgtag cgaagggcca gcgccgcgtc gatctccgcc ttggcgcgcg
      121 caaacggttc gcgcgtggcg aggtcgaggc ggtcgaagag cgcggacatg tcatccgcct
      181 gctgttcgga cagctcgagc gacatgacgt agtaatcggc gaaaccgagc gatcgcgcga
      241 cctcgttgcg taaccggacc aatcggagga ggtcgtcgcg cacctcgcgg ccgaccgcct
      301 tgtcggcctc ccacacgcgc ctccgatagg ccgcgtcggt gctctgcttg agcgcgttgc
      361 gcacgtcgtt gcccgtcagc gatcgcccgt cgacgtgcgc tcgataggtt ccgaacgact
      421 gcacgatctt gctggatagg cgcgtcatct cttcgatgtg ggcggggtcg atttggttgc
      481 ggaggtacac gcgatacagg aggtcgagtt gtcgccgctc cagaccgtcc acgccggagg
      541 ccgccagcca ccccttcacc tcttcgaact cgcgcgcgct cgtgtagacc ttctgcaact
      601 cgacctcgag ccgtgcgtag cgttcgaagt cttcgggctt ccccgagatg gtcgcgctcc
      661 aatacgcgag gttgacgtcg cgcgagagtg gctcgatgcg agcgacgtgg gcgtcgagga
      721 atgagcggac ggcggtcgtc atggacgctt cctggggttc ggaatgggaa ccgcgcaccc
      781 ggcgggcgcg cgggaggtcg gcggggagac ggtaacccac gccggcgtca tcgcgctaga
      841 ataaaaatgg caattgtttt cgtagcaatg ggtttcggtc ggccgtagaa tcgcgtgcaa
      901 gattgcgccg aatttggccc gggccgcgcg tgtaaccgag cgcgtggaag ccagtctacg
      961 cgccgcggcc gggccggcac tgccggagct cccgttgaac gtcgagaacg aacgcgaaat
     1021 cattcgcggg tggaagcgcg gcgacaaacg cgcctacgaa gccctggtcc gccgctacat
     1081 gacggatgcg tatctcgtcg cctacggata cgtgggcaac gccgaggacg cccgcgatct
     1141 gtcgcaagag gccttcgtca aggtctacca agcgcgcgct cgcttcgatg aggagcgccc
     1201 cttctatccg tggttgtacc gagtgctgcg caaccactgt ctcaatttcc tgcgccgtgg
     1261 gcgcgtcgag gtctcgatcg atgacgagaa cgcccatcgc gagatcgcgt cgccgcgacc
     1321 gacggtgctc gacgacatgg agagcgacga gcgccggcgc atcgtgcgct cggctgtcgc
     1381 gcgtctgtcc gacgaccatc gggagatcat cgtgctgaag aacttcaagg atcattcgta
     1441 caaggaaatc gcggagatac tggaaatccc gatcgggacc gtgatgtcgc gcctgttcta
     1501 cgcccggcag gcgctgcgcg cactggtcga agagatcgag ggccgaggcc ctatcaagga
     1561 ggaggcgttt tgaccccggc ctgcacgcgc tatcaaagcg acggcatgcg ctggctcgac
     1621 ggtgagctca cggcgcagga agccgatcac taccaggccc acgtgcgcga gtgcgacgag
     1681 tgccggcgcg agctcgcgtc cctgggccgc gtcgtacgca tgaccgacga gctcaagctc
     1741 cgcgcccccg acgacgaatt ctggaagggc tattgggaga gtgtctatcg tcgcagcgag
     1801 cgccgcgtcg gcttcgtgct actgatcgcc ggcgttctcg cggtgttggg ctacgcgata
     1861 tttcgcgccg tcacgtcgcc ggagttgctg acctacgagg gcatcagcgt gatgctgatc
     1921 ttgggcggcc tggtcgtgat cttcatttcg gtcgcgcgcg agcgctacca cgagcacaag
     1981 agcgatccct acaaggaggt cgagcgatga tcgtcgtgac caccgacgac gtgccgggac
     2041 accgcgtgac gcaggtgttc ggactcgtcc aaggcagttc ggtgcgcgct cgacacgttg
     2101 gaaaggacat cctcgcctcc ttcaagaacc tggtcggcgg cgagatccac gaatacacca
     2161 agctgctcgc ggagtcacgc gagcaggcgc tcgatcgtat gcgcgaggag gctgcgcgca
     2221 tgggcgccaa cgccatcgtg ggcatgcgat tctcgtcgac tgacatctcc caaggtgcct
     2281 ccgagttgct cgtatacggg accgcggtgc agatcgagcg cgcgtagacc cctgcgtttg
     2341 ccggccgttt gcgcaccgtg atatagtgcg tgcatgcgtt tcggcgattg gaacgttcgc
     2401 acgtgcatcg cggggcgatt cggtttggac ggcggcgcga tgttcggtgt cgtgccgaag
     2461 accatgtggg agcgcctgct cccccccgat tcgtacaacc gcgtgcccat ggtgatgcgc
     2521 accctgctgg tgcaggggca cggcaagacg gtggtggtcg acgtgggcgc gggcggtggc
     2581 cacagcgaga agaaccggca gatctatgcg ttcgaagaca ccgaccacct gaccgacgcg
     2641 attcgcgcgc acggtgtgga gcccgcggag gtcaccgacg tgctcctgac ccacctccat
     2701 ttcgaccacg gagccggcat cgtcgagcgc gacggggatg actggcggct ggtttttccg
     2761 cgcgcggttc atcatttgca gacgagccag tggaagcacg cgctctcacc caacccgcgc
     2821 gatcgcgcga gctattacga cgaccgcatt cgcgtgttgg aacgcgagaa ggtgttgcgg
     2881 ctctacgacg gcccctggtc gctctcaccc gggttcgacc tgctggtctt cgacggacac
     2941 acgccgggcc agcagctccc agtcatcgga ggaggggtgg gatcgctctt cttctgcggc
     3001 gacctgtttc cgacccatca tcatattccc accccctacg tcatgagtta cgacctctat
     3061 ccggtcacgg cgatggagga gaaggcggcc ctcctggagc gggccgaagc gggcaagtgg
     3121 ttgctgtgct tcgagcacga cgcccacatg gaggcctgcc gggtgacgcg ggaggggaaa
     3181 cggttcactg ccggagagac cgtccgaatt ttgtgagaac catcggccgg ggacggtgta
     3241 gaagttgcac ccggccgctt gacggccgcc ccgctttgga tgatcattgg gtggcccgca
     3301 accgggttca ccccgaatcg accaccttca ggaggcgttg gaaatgacac ggaagattat
     3361 gacgctcgga accgtagctg cattcggtgt ggccgcggca ctcgtcggag ccggcgacgc
     3421 cggacttgcg ttcgcgtgtg acaaggcgac caagaccgcg gcggcgacga cctcgtcgtc
     3481 ggattgttcc aaagacgcca ccaaggcgtc ggccacgacc gcatcggtca agacggcgtc
     3541 ggcgtcgtgt tgcgcgaagg atgccaagac gacgaccgcc ggcaacgtcc ataagtcgat
     3601 gatcaaatcc gcggtggttg caccgggtgc ggcgcccgtg ctgaacacgg ccgcgttcgc
     3661 ggcgggtgtc agcggctcga cggtggctgc gggcagcgga agctgctgcg agtccaagtc
     3721 ggccagcgcc gcgtcggcgg cgggtgcgtg cgacaagtcg gctgcggtga agaccgcggg
     3781 cgcgggcggc tcgtgcgaca agtcggcggc ggccaagacg gccggtgcgt cgggttcgtg
     3841 cgagaagtcg accaagacgg cggccgcggc ggtcgaccaa gtcccgtatc gcgagagcaa
     3901 gcgcgtcgtg ttgaccggca cctacgagtg cggtcactgc ggcatcggcg cgactgccga
     3961 gtgcgccccg atgttcaaga cggcggatgg caaggtgtat ccgctgtgga acagcgatcg
     4021 cgtgtcgagc ctgcgcaacg acaaggcggc caagaacgtc gagatcgcga ccctcgtgaa
     4081 gaaggtcgac ggcgtgaagt acctcgacgt caagagctac aagaccctgt aagaaccgac
     4141 agcgcgtccg ccacggacgc gtttacggca tggaacgacg aaggcctccc gatccgggag
     4201 gccttctctt tttgcatgat gactggaacc tgcgctactt gccgcgcccc cgggaccctc
     4261 cccgcgatcc gccgctcggc gccgatcgag gcgcggagcc cttcatcgat ccgccgcggg
     4321 acgacggtgc gctgtaggag cgcgacggcg ttgacttcgc gcgcggtgcg gacgacggcg
     4381 acgaacccac gcggcctccg ctgggccgcg acgagccccg ggttccgggc gcaacgctgc
     4441 gcgacttgta tccgccgcct ccgcgctgca cgtcggccac gcctttgcgg gacgatccgg
     4501 tcgaccggac gcggggtgtc gtggcgctgc cccgtgcggc gccgccgcgc gtcgtgcccc
     4561 acttcgcctg ccccgagctc cggccgccga tcgacgtctt cacgcgcggc gaggcatcga
     4621 tggcgcctcg gctcttgccc gatccgaaag tcgtacgcgt ggcggtcccg gtcttcgagc
     4681 ggacggacga ctgcgcgggg cgcgaaagcg actgctggac gtccttcgat gcgatgcgca
     4741 gtgagccgtc gcgcttctcg gcgacggcgc gcgtcccggt cagagtcgac gccgattcgg
     4801 acttgtactt ggccttgaat ttctccgggt acgcgtacga gccggagccg tagtagccgt
     4861 catagccgta gccccacccg cacggcgcat accagcccga gcagtagtag ccgccccacc
     4921 atccccagcc ccaaccccag ccgatcgaga aagaccatcc gggcccatag tacggatagt
     4981 acggtcc
//
LOCUS       DASPGK010000239         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1583696, whole genome shotgun sequence.
ACCESSION   DASPGK010000239 DASPGK010000000
VERSION     DASPGK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583696"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VEC56_06525"
     CDS             <1..477
                     /locus_tag="VEC56_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXV13844.1"
                     /translation="YARWLKRVWPAGNLAVAAISASALFGGAMVAGRPELSVLPMAIA
                     FAFVLCRELVKGAEDVEGDRAAGARTLAVIAGRHRAARVASTAMMLVAVALPLPAVLG
                     VYRPAYAWIMLGLVVPALVAGAVTATQARDARRFARVSRLLKLAMFAGLAAIVAGT"
     gene            complement(503..1168)
                     /locus_tag="VEC56_06530"
     CDS             complement(503..1168)
                     /locus_tag="VEC56_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV13845.1"
                     /translation="MNPSRNFVCGIIALGLPLFAQDASAVRYAERPLLWNRTAVSGYA
                     GAGYPVGNFASDDLDYDGAHEANWPLDWSVEVEHFVGRTWSLGFSAAYTTYEDKDFPE
                     LETNLNTYSGFLRIVVPSGTDIRPYFRAGMGAMQLESEVDGAFRFDAEYVFSFQAGAG
                     LLWLPQRWLGLNLQALYYYGSTEDAYYGPDASVYFEEDVPIAVGYDTTYFVFAAGVSL
                     FFP"
     gene            complement(1267..2676)
                     /locus_tag="VEC56_06535"
     CDS             complement(1267..2676)
                     /locus_tag="VEC56_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXV13846.1"
                     /translation="MTHSAAWAQYQLENAFPNLSFDTAVDIQALGSINSQNRLYVAEK
                     RGVIYTFPEDIQVDPGQRTVFLDLTTKANDSGEAGLLGLALHPNFETNRYFFVYYVTG
                     FPYRNIVARYRATADFSAADPQSELILIDEPKNTLFHNGGQLAFGPGNLLYIAMGDDT
                     QSSNGQDLTDLFGSILRIAPNVAGDSPAYTIPNGNPFKGNPSGSREEIFAYGFRNPWR
                     FSIDPGTGDVWVADVGENTYEEVDLVESGGNYGWPLMEGPDCFQPASCDTAGKGLSLP
                     IESYTHGEGSAVIGGHVYHGTRLPELEGLYVFGDFSGAVWTLSYDGVNPPVRSDLATT
                     GSLLLTIGVGNMPKRDVYLSSFDGSIYRVSRVVTDTGRAPDAAGNRLLGNFPNPFNPA
                     TTIRYQLAQPGRVAVEILAVDGSRVRMLEQGSRDAGAHDLTWRGETDAGGRAASGVYF
                     YRLLLDGVARDTARMVLVQ"
BASE COUNT          479 a          875 c         1010 g          487 t
ORIGIN      
        1 tacgcgcgct ggctcaagcg ggtgtggccc gccggtaacc tcgcggtggc cgcgatctcg
       61 gccagcgcgc tcttcggggg ggcgatggtg gccggccgcc cggagctctc ggtgcttccc
      121 atggcgattg cgttcgcgtt cgtgctgtgt cgcgaactcg tcaagggggc ggaagacgtg
      181 gagggcgacc gcgccgcggg cgcgcgcacg ctggcggtga tcgcggggcg tcaccgggcg
      241 gcgcgcgtcg cgtcgaccgc gatgatgctg gtcgcggttg cgctgccgtt gccggcggtg
      301 ctcggggtct atcgacccgc gtacgcgtgg atcatgctgg gcctggtggt accggcgctc
      361 gtcgcgggtg ccgtcacggc gacgcaagcg cgcgacgcgc gccgcttcgc gcgcgtgagc
      421 cggctgctca agctcgcgat gttcgcgggg ctggcggcca tcgtcgcggg aacgtagcca
      481 gcgccgtagc gcggcgcttc gatcagggga aaaacagact cacaccggcg gcaaatacga
      541 agtacgtggt gtcgtagccg acggctatgg gaacgtcttc ttcgaaatac acggacgcgt
      601 cgggaccgta gtacgcgtcc tccgtcgagc catagtagta gagagcctga aggttcaagc
      661 cgagccaacg ttgcgggagc cacaagagtc ccgcgccggc ttggaacgag aagacgtatt
      721 cggcgtcgaa gcggaatgcc ccatctacct cggattcgag ctgcattgcg cccatgcccg
      781 cgcgaaagta cgggcgaata tcggtgccgc tggggacgac gatgcgaagg aatccagaat
      841 acgtgttgag gttggtctcg agttctggga aatccttgtc ctcgtaggtc gtgtacgccg
      901 cggaaaaccc cagggaccag gttcgaccca cgaagtgctc cacctcgacc gaccagtcga
      961 ggggccagtt ggcctcgtgg gctccgtcgt agtccaagtc gtcgctcgcg aaattgccca
     1021 cgggatatcc cgcccccgca tagcccgaca cggcggtgcg attccaaagc agcgggcgct
     1081 ccgcgtagcg cacggcgctc gcgtcttgcg cgaacaaagg gagccccaat gcaataattc
     1141 cgcaaacgaa gttacgcgat gggttcatgc caagcttgcc tctctacggg gtggtgcagt
     1201 cggggcggcg actcgctggt cttaagattc gcggctggcg gcgcgcctgt caaccgaaac
     1261 ggcggcttac tggaccagca ccatgcgcgc ggtgtcccgg gcgaccccgt cgaggagcag
     1321 gcgataaaag tagaccccgc tggccgccct cccgcccgcg tcggtttcgc cgcgccacgt
     1381 gaggtcgtgg gcaccggcgt cgcgcgagcc ctgctccagc atcctcacgc gcgacccgtc
     1441 caccgcgaga atctcgacgg ccacgcggcc cggctgcgcg agctggtagc ggatggtggt
     1501 ggcggggttg aacgggttgg ggaaattgcc gagcaggcgg ttgccggccg cgtccggcgc
     1561 gcgcccggtg tcggtgacga cgcgcgacac gcgatagatc gacccgtcga acgacgagag
     1621 atacacatcg cgcttgggca tgttgccgac tccgatcgtc aagagcagcg acccggtggt
     1681 cgcgaggtcg ctgcgcacgg gcgggttgac gccgtcgtag ctcaacgtcc acaccgcgcc
     1741 gctgaagtcg ccgaacacgt agagaccctc gagttcgggc aaccgcgtcc catggtagac
     1801 gtggccgccg atcacggcac tgccttcgcc atgcgtgtac gactcgatgg ggagcgagag
     1861 gcccttgccc gcggtgtcgc acgaagcggg ttggaagcaa tcgggtccct ccatcagcgg
     1921 ccagccgtaa ttgccgccgc tctcgacgag gtcgacctct tcgtaggtgt tctcgccgac
     1981 gtccgcgacc cacacgtcgc cggtcccggg gtcgatcgag aaacgccacg gattgcggaa
     2041 accgtacgcg aagatctcct cgcgagatcc gctggggttg cccttgaagg gatttccgtt
     2101 gggaatggta tacgcgggac tgtcgcccgc gacattgggc gcgatgcgca ggatggaccc
     2161 gaacaggtcg gtgaggtcct gcccgttcga cgattgcgtg tcgtcgccca tcgcgatgta
     2221 caggaggttg ccggggccga acgcgagttg acccccgttg tggaacaggg tgttcttggg
     2281 ctcgtcgatc agaatgagct cgctttgcgg gtcggcggcc gagaagtcgg cggtggcccg
     2341 gtaacgcgcg acgatgttgc ggtacggaaa tcccgtcacg tagtagacga agaagtaccg
     2401 gttggtctcg aagttgggat gaagcgcgag tcccagcaag ccggcctcgc cggaatcgtt
     2461 cgccttggtc gtcaggtcca ggaacacggt gcgctgcccg ggatcgactt ggatgtcctc
     2521 ggggaaggtg tagatcacgc cccgcttctc ggccacgtag aggcggttct gggagttgat
     2581 cgacccgagt gcctggatgt cgaccgcggt gtcgaacgac aggttgggga acgcattctc
     2641 gagctggtac tgggcccagg ctgcggaatg agtcatcgcg acgacgagcg gaatccaggc
     2701 gaacgaacga cggatgcgca ctgtgtacct caatgggtgg gatgcgtcgg tatgaccgtg
     2761 gggcggtacg gccgggccct tgtactgcgc ctgcgccgtg gcgcgcgcga aaggactcca
     2821 ttataatggg cgcttcgcgg ccggtcaaac c
//
LOCUS       DASPGK010000240         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_764184, whole genome shotgun sequence.
ACCESSION   DASPGK010000240 DASPGK010000000
VERSION     DASPGK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764184"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VEC56_06540"
     CDS             <1..1380
                     /locus_tag="VEC56_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13847.1"
                     /translation="AQLTGGPHVFYWKGRRWDGSLATEGAYVVTLFARGAANPDSVKS
                     LPVFVDVTRPTVQIVSVLPNPYAPGAPGSTASVSFSFAIADASPVFPGRVPDELRSTF
                     AYPSGAAFTPVSLTTTPPYTGASGAYVLSWNATAEAAVPPDGEYRVTLTLNDVAGYTA
                     TSTYLFDVDTRAPDVEITSLSENASVSTVPDSLRGYAFDKRGVDSLLVRYADTRPYQP
                     VVSTWLSGDTLGFSVPLADSFPTEGAHRVDFRAVDVFGRAVESFFNFRSDATAPPAPV
                     LDPFVGGSWKTSRYPLTGRADDGGDASFVRVYRNGALVDSVSTFVSDDFEVEVALVPG
                     RNDLVAVLRDAAFNASPPSNTVTVDFDTGAGFFAPAPFGPGDAFDVNANGTASRCTLR
                     LFDLTGDLVIVLEDNGARQFYAFAWDGTNGSGKSVKKGPLVAVASLDYPDGTHDVFRE
                     VFLYDPDAP"
     gene            1377..2297
                     /locus_tag="VEC56_06545"
     CDS             1377..2297
                     /locus_tag="VEC56_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13848.1"
                     /translation="MTTRARLFVLVALCAGLVPARARAESSPGGAFRLPAYGARGWGM
                     AGAFVARVDDESAVDWNPAGLGIAPRSAGASYLQLIEGVSAGQSQIVFTMPLSRERHE
                     NGAARHAAGAMFTNLSADVVGGESYSENYLRVAYALTPEPLVSFGLAATAFMSSSGVP
                     GFDAWGTSFDISGMLSLSRAWSMALVARDAFSRYSYDDGRDYEKEPQYVVGLAWQRAK
                     RFAVEGDVVRSYGGWSRASLGVESEYFLDHLALRGGIAWLSAGESRTVPSFGASVRAL
                     SNRLTLHYGATLDEEDAFGRTHRVSLAVGI"
     gene            complement(2299..3597)
                     /locus_tag="VEC56_06550"
     CDS             complement(2299..3597)
                     /locus_tag="VEC56_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV13849.1"
                     /translation="MSEAGAGRRRAAWVAILAVAALARLVFAGAVVGWDTPPKADEAD
                     YHAIAVNLLEGDGFVSFEGTPTARRPPGYPVFLAAVYAIAGESPRAGRVVQVILGVVV
                     VALTGLLARRYFGESIALLAAGLSAINPFLIFISGYLLTENLYMVLLLAALVTARDPR
                     TLAASHARAAATGVILALATLARPTGLPMLEWVLAALLALSGVAWRARLVRGALAAAA
                     FALVVLPWYARNASVVGGWVLTTHGGITFLQGNNEKVASVPQWRGGAAPLEVLPRFDE
                     MAGLDEFTRDRLAFELGYHYVQTHANDVPELVFWKMVRFWRLHSDMGLSGVKSGWWWS
                     KNSTLGRLAADIDVGFAYAIVAVPLFVLGVVVTFRRWRELSLLLGVVAVHTAVAMVFY
                     GSLRSRIPVEPVMCVLAAVALAAIVGRVRARGGRVATAPG"
     gene            complement(3594..>3978)
                     /locus_tag="VEC56_06555"
     CDS             complement(3594..>3978)
                     /locus_tag="VEC56_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV13850.1"
                     /translation="RAAGHGFFIQPTVFGDVPPDATISQEEIFGPVLALIRAQNFDDA
                     LAIANNTEFGLTGAVYSNNREHIERARREFHVGNLYFNRKCTGALVGVHPFGGFNMSG
                     TDSKAGGRDYLLLFTQAKTTSEKIR"
BASE COUNT          614 a         1396 c         1360 g          608 t
ORIGIN      
        1 gcccagttaa cgggcggtcc gcacgttttc tactggaagg ggcggcgttg ggacggctcg
       61 ctcgcgaccg agggcgccta cgtcgtgacg ctgttcgcgc gcggcgccgc caatccggat
      121 tccgtcaagt ccctgcccgt cttcgtcgac gtgacccggc ccacggtgca gatcgtgagc
      181 gtgctcccga acccgtacgc gccgggtgcg cccggctcga cggcgtcggt gagtttttcg
      241 ttcgcgatcg ccgacgcctc gccggtgttt ccggggcgcg tgcccgacga gctgcgcagc
      301 acgttcgcct acccgagcgg cgccgcgttc acacccgtct cgctcacgac cacaccgccc
      361 tacaccggtg ccagcggcgc gtacgtgctg tcgtggaacg cgaccgcgga ggccgccgtt
      421 cccccggacg gcgagtatcg cgtgacgctg accttgaacg acgtcgccgg ttacaccgcc
      481 acctcgacct acctgttcga cgtcgacacg cgcgcgcccg acgtcgagat cacgagcctc
      541 tccgagaacg cgtcggtgag cacggtgccg gacagcctgc gcggctacgc cttcgacaag
      601 cgcggcgtcg actcgctcct ggtccgttac gcggacacgc ggccctacca gccggtggtg
      661 tcgacctggc tctccggcga cacgctgggc ttctcggtgc cgctcgccga ttccttcccc
      721 accgaaggtg cgcaccgcgt ggactttcgc gccgtcgacg tattcgggcg cgcggtcgag
      781 tccttcttca atttccgcag cgacgcgacg gcaccccccg cccccgtgct cgaccccttc
      841 gtcggcggga gctggaagac cagccggtat ccgctcacgg ggcgcgccga cgacggcggc
      901 gacgcgtcct tcgtgcgcgt gtaccgcaac ggcgcgctgg tcgactcggt gtcgacgttc
      961 gtgagcgacg acttcgaggt cgaggttgcg ctggtacccg gccgcaacga cctggtggcg
     1021 gtcttgcgcg acgccgcctt caacgcgagt ccgccttcca acacggtgac ggtcgacttc
     1081 gacaccggtg ccggtttctt cgcccccgcg cccttcggcc ccggcgacgc gttcgacgtc
     1141 aacgccaacg gcaccgcgag ccgctgcacg ctccgactgt tcgatctgac cggcgacttg
     1201 gtgatcgtat tggaagacaa cggggcccgg cagttctacg ccttcgcgtg ggacggtact
     1261 aacgggagcg ggaagagtgt caagaagggc ccgctggtcg cggtcgcgtc gctcgactac
     1321 ccggacggca cgcacgacgt gtttcgtgaa gtgttcctct acgacccgga tgcgccatga
     1381 ccacacgcgc gcgactattc gtgctggtgg cactgtgcgc ggggctggtt ccggcccgcg
     1441 cgcgggcgga gtcgtcgccc ggcggcgcgt ttcgactgcc cgcgtacggc gcgcgcggct
     1501 ggggcatggc gggcgcgttc gtcgcccggg tcgacgacga gagtgcggtc gactggaatc
     1561 ccgcgggcct cggcattgcc ccgcgctcgg cgggggcgtc gtacctccaa ctgatcgaag
     1621 gcgtctcggc cggccaatcg cagatcgtgt tcaccatgcc gctttcgcgc gaacgccacg
     1681 agaacggtgc ggcgcgccac gcggccggcg cgatgttcac caatctgtcc gcggacgtgg
     1741 tgggaggcga gtcgtacagc gagaattacc tgcgcgtcgc gtacgcgctg acacccgagc
     1801 cgctggtgtc gttcgggttg gccgcgacgg cgttcatgtc ctcgagcggt gtgcccggct
     1861 tcgacgcgtg ggggaccagc ttcgacatat cggggatgct gtcgctctcg cgcgcgtggt
     1921 cgatggcgct ggtcgcgcgc gatgcgttct cgcgctacag ctacgacgac gggcgcgact
     1981 acgagaagga gccgcagtac gtggtcgggc tcgcgtggca gcgcgcgaag cgattcgcgg
     2041 tggaaggcga cgtcgtgcgc agctacggcg ggtggtcgcg cgcgtccttg ggcgtcgaga
     2101 gcgagtactt cctcgatcac ttggcgctgc ggggcggtat cgcgtggctg tcggccggcg
     2161 aatcgcgcac ggtgccgtcg ttcggcgcca gcgtgcgcgc gctctcgaac cgattgacgc
     2221 tccactacgg cgcgaccctc gacgaagaag acgccttcgg gcgcacccac cgcgtctcgc
     2281 tcgcggtcgg tatctagcct accccggcgc ggtcgccacg cggccgccgc gcgcgcggac
     2341 acgacccacg atcgcggcca acgccaccgc cgccagcacg cacatcaccg gctcgacggg
     2401 aatgcgcgaa cgcagcgacc cgtagaacac catcgcgacc gccgtgtgca ccgccaccac
     2461 gccgagcagg agcgagagct cgcgccagcg ccggaaggtg acgactacgc ccagtacgaa
     2521 caacggaacc gccacgatcg cgtacgcgaa gcccacgtcg atgtcggcgg cgaggcggcc
     2581 cagcgtcgag ttcttgctcc accaccatcc gctcttcaca ccgctcaggc ccatgtcgct
     2641 gtgcaggcgc cagaatcgca ccatcttcca gaacaccaac tccggtacgt cgttggcgtg
     2701 ggtttgcacg tagtggtacc ccaactcgaa cgccaggcga tcgcgcgtaa actcgtcgag
     2761 ccccgccatc tcgtcgaagc gcggcaacac ttccaagggc gccgcgccgc cgcgccactg
     2821 cgggacgctc gcgaccttct cgttattgcc ctgcaggaag gtgatgccgc cgtgggtcgt
     2881 caatacccag ccgccgacca ccgacgcgtt gcgcgcgtac cacggcagga cgacgagtgc
     2941 gaaggcggcg gccgcgagcg cgccccgcac cagacgcgcg cgccacgcga cacccgaaag
     3001 cgcgagcaac gcggccagca cccactcgag catggggagc ccggtgggac gcgcgagcgt
     3061 agccagggcg aggatgacac cggtcgccgc cgcgcgcgcg tgcgatgccg ccagcgtgcg
     3121 cggatcgcgc gcggtcacga gtgcggcgag caacagcacc atgtacaggt tctcggtcag
     3181 caggtagccc gagatgaaga tgagaaaggg attgatcgca gaaagtccgg ccgcgagcag
     3241 tgcgatggat tcgccgaaat aacggcgggc gagaaggccc gtgagtgcca ccaccaccac
     3301 gcccaggatc acctgcacga cccggccggc gcgcgggctc tcgcccgcga tcgcgtagac
     3361 cgcggcgagg aacacgggat aaccgggcgg gcggcgggcg gtcggggttc cctcgaagga
     3421 gacgaagccg tcgccttcga ggaggttgac cgcgatggcg tggtagtcgg cttcgtcggc
     3481 cttgggcggg gtgtcccacc ccactaccgc accggcgaag acgagcctcg cgagtgcggc
     3541 gacggcgaga atcgcgaccc acgccgcgcg acggcggccc gccccggctt cgctcaccgg
     3601 atcttttccg acgtcgtctt ggcctgcgtg aacaacagca gatagtcgcg accgcccgcc
     3661 ttgctgtcgg tcccggacat gttgaacccg ccgaacgggt gcacgcccac cagcgcgccg
     3721 gtgcacttcc gattgaagta cagattaccg acgtggaact cgcggcgcgc gcgctcgatg
     3781 tgctcgcgat tgttggagta caccgcaccc gtcaacccga actcagtgtt gttggcgatc
     3841 gcgagggcat cgtcgaagtt ctgggcgcga atcagcgcca gcacggggcc gaagatttct
     3901 tcttgcgaga tggtggcatc gggcggcacg tcgccgaaca cggtgggctg gatgaagaaa
     3961 ccgtggcccg cggcgcgc
//
LOCUS       DASPGK010000241         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_529911, whole genome shotgun sequence.
ACCESSION   DASPGK010000241 DASPGK010000000
VERSION     DASPGK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529911"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VEC56_06560"
     CDS             complement(<1..409)
                     /locus_tag="VEC56_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13851.1"
                     /translation="MPLNAPYRLPILALGCATAMLSCSGGARDAADPVARAQSHLTSG
                     DAASATAVLETGGARDAVDPAAACLLGRLYRERGTIQGRLLSQGVLETARARHPDDLD
                     VVLELAKTNFAQGFYPDAVRALRKVLAQDPARCD"
     gene            complement(594..968)
                     /locus_tag="VEC56_06565"
     CDS             complement(594..968)
                     /locus_tag="VEC56_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13852.1"
                     /translation="MKRSQLFVFVLLVLAAVPAWAQGPTAAKAVTGMEDGVTTIAITV
                     ASKGDEVYGVNIKGAKLEDIRAPKGWVGISSGSSVMFRTGENPVKSGNSLAFKLMTTD
                     PSATMTVSFRGKDDSVGQPVNL"
     gene            1272..2027
                     /locus_tag="VEC56_06570"
     CDS             1272..2027
                     /locus_tag="VEC56_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13853.1"
                     /translation="MFTKRFPRSAGRSLGIAVLALLLPAIVPGEVVRLKDGSTLKGRL
                     MRVEGDTLTIRLSVGAPIKIHRSVVESIVFSDSIPPSPPAQAVVSGAGVPPADPVGVG
                     TVSIKFEDRNVSSKITIDKKKHWDEKVASNAIVVEFVVDGVVKYTAIDTTTDKTIYLG
                     HDKQLKNDTELADFDVQVPAGTRRCSLVLRNQDPDTFRDSFDPAPLNAVLDLGETYVR
                     PGGLVRVDVRIDKGLLRTSSPKLYRGNHVEKDD"
     gene            complement(2029..3150)
                     /locus_tag="VEC56_06575"
     CDS             complement(2029..3150)
                     /locus_tag="VEC56_06575"
                     /inference="COORDINATES: protein motif:HMM:NF020855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13854.1"
                     /translation="MFPILLLAIAALAGCRDAAIQHYNLGIDALERGDTTTAVSEFER
                     SIAQRFEDPDAHMNLGVALLGRGDAERALSEFQIAARYTPDDVDLHVNMAEAYRALGR
                     TQAARTEYEWALKRAPENVAALSGYGRLLMDGGSMEAAHGQFVKALAADPGHAPTMFH
                     MGWLYLQTGRPTEATHYFLRGLQIAPKALYGRLGLAEAYQVRGMDAEALTEFQKAYVA
                     DSTSVEAMVGIGKCQTRTGNYAVAERILEKANVRAPDDPRVLTLLADVYLHRDRRAEA
                     VAYYRRAIQIDENNADAYLGLGTALEAAGELDAAEEALQSALLHAPKNAAVMYRLGMV
                     YLDKNEKTRARAYFEMAMDAVGNDAALKTNIRTGLDESR"
     gene            complement(3231..>3697)
                     /locus_tag="VEC56_06580"
     CDS             complement(3231..>3697)
                     /locus_tag="VEC56_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13855.1"
                     /translation="RRYAPCSSPGVMPPKLDNRVIARYLDGTLVRGTTFDFAPTKTSF
                     HVDDGHGTREIDVGDLKALFFVRDFEGDSGYHEKKGFFSTYPHGKKVMVEFLDGEVLF
                     GYTLSYSSRGVGFFVFPGDPDSNNEKVFIVHGATKRVKLRTAGGQPDFPYAR"
BASE COUNT          595 a         1177 c         1281 g          644 t
ORIGIN      
        1 cgtcgcaccg cgcgggatct tgcgcgagta ccttgcgaag ggcacggacc gcgtcggggt
       61 agaagccctg cgcgaagttg gtcttggcca gctccaagac gacgtcgagg tcgtcggggt
      121 ggcgcgcgcg cgcggtttcg agcactccct gcgacaggag tcgtccctgt atggtgccgc
      181 gctcgcgata aagacgtccc agcaggcacg ccgcggccgg gtcgaccgcg tcgcgcgctc
      241 cgccggtttc gagcacggcg gtggcggagg cggcgtcgcc ggaggtgaga tgggattgcg
      301 cgcgcgcgac cgggtcggcg gcgtcgcgcg ctccgcccga gcacgaaagc atcgcggtcg
      361 cgcagccgag cgcgaggatg ggcagacgat agggcgcgtt cagcggcatg tgtcgggctc
      421 cagccgtgcc cgcggggtag gcggcgcccg taatataggc gggtgcgggg ccccggggaa
      481 gcgtcgatca cactcgacac ggccgtccga cgatttcatc cccccaaaaa ctcgcggcgc
      541 ggtgacgaat tgcttcgccg ccgcgccgct cgtgcgggac cctggcccct atgctagaga
      601 ttgaccggct ggccgaccga gtcgtccttg ccgcggaagg agaccgtcat cgtggctgac
      661 ggatccgtgg tcatgagctt gaaggcgagc gagttgcccg acttcaccgg attctcgccg
      721 gtgcggaaca tgacgctcga accggacgag atgccgaccc aacccttggg cgcgcgaatg
      781 tcttccaact tcgcgccctt gatgttgacg ccgtagacct cgtcaccctt ggacgcgacg
      841 gtaatcgcga tggtggtgac gccgtcttcc atgcccgtca cggccttggc ggcggtggga
      901 ccctgggccc acgccggcac ggcggcgaga acgagcaaca cgaatacgaa taactgggac
      961 cgcttcatga gggatcgacc tcctatggat gctatgtcga agatgcgaat acggtcattc
     1021 ggatcgatca cgaacggaac gctccatcgt cgccccgggc ggtgcaccgt tccccggggg
     1081 tctcacggag gtcccgtggt tgaattgatg gtcgaattgt accacagcgc ggccgtcgct
     1141 gtcgaggttt gtggcgtcgc tacgcgaaaa tcccggttcg tccccgagcc gggcgcattc
     1201 gccttgtacc gcttccggac ctcccgttat catgccggcg gcgttcctta ccccgatcgc
     1261 ggaggttagc ggtgttcacg aaacggtttc cacgctcggc cgggcgctcc ctggggatcg
     1321 cggtgctggc gctgctgctg ccggccatcg tccccggaga agtggtccgg ctcaaggacg
     1381 gctccaccct caagggccgc ctgatgcgcg tggaggggga caccctgacc attcgcctga
     1441 gcgtcggcgc gcccatcaag atccaccgca gtgtcgtcga atcgatcgtc ttttcggact
     1501 ccattccccc gtctcccccg gcgcaggcgg tggtttccgg ggccggggtg ccccccgcgg
     1561 acccggtcgg cgtggggacg gtgtccatca agttcgagga ccgcaacgtg tcgagcaaga
     1621 tcacgatcga caagaagaag cactgggacg aaaaggtggc gtcgaatgcc atcgtggtcg
     1681 agttcgtcgt cgacggtgtc gtgaagtaca ccgcgatcga caccaccacc gacaagacca
     1741 tctacctcgg gcacgacaag cagctcaaga acgacaccga gctcgcggac ttcgacgtcc
     1801 aggtgccggc cggaacgcgc cggtgctctc tcgtcttgcg caatcaggat ccggatacgt
     1861 ttcgggattc gttcgatccc gcgccgctca atgccgtgct ggacttggga gagacgtacg
     1921 tgcgcccggg tgggctggtg cgcgtggacg ttcgcatcga caagggcttg ctgcgaacgt
     1981 cgagcccgaa gctctaccgc ggcaaccacg tcgaaaagga cgactgactc agcgcgattc
     2041 gtcgaggccg gtgcggatgt tggtcttgag ggcggcgtcg ttgccgaccg cgtccatcgc
     2101 catctcgaaa taggcgcgtg cacgcgtctt ctcattcttg tccaggtaca ccatacccag
     2161 tcgatacatg accgcggcgt tcttgggggc gtgcagcagc gcggactgga gcgcctcttc
     2221 ggcggcgtcc agctcgcccg cggcctcgag cgcggtgccg agacccaagt acgcgtcggc
     2281 gttgttctcg tcgatctgga tggcgcgccg atagtacgcc accgcttcgg cgcggcggtc
     2341 gcgatggagg tagacgtcgg ccagcagggt cagcacgcgc gggtcgtcgg gagcgcggac
     2401 gttcgctttc tcgaggatgc gttcggcgac cgcgtagttt cccgtgcgcg tttgacactt
     2461 gccgattcca accatcgcct cgacgctcgt cgaatcggcc acgtacgcct tctggaattc
     2521 cgtcagcgcc tccgcatcca ttccgcgcac ctggtacgcc tcggccaagc ccaagcgccc
     2581 gtagagtgcc ttgggcgcga tctgcagccc gcgcaggaag tagtgcgtcg cctccgtggg
     2641 gcggccggtt tgtaggtaga gccagcccat gtggaacatg gtgggcgcgt gcccggggtc
     2701 ggcggcgagc gccttgacga actggccgtg cgccgcctcc atcgagccgc cgtccatcag
     2761 caatcgtccg tagcccgata gcgccgccac gttttcgggc gcgcgcttga gcgcccactc
     2821 gtattcggtg cgcgcggctt gggtgcggcc gagggcgcgg taggcctccg ccatgttgac
     2881 gtgcaggtcg acgtcgtcgg gggtgtagcg cgcggcgatc tggaactcgg agagagcgcg
     2941 ttcggcgtcg ccgcggccga gcagagcgac tcccaggttc atgtgcgcgt cgggatcctc
     3001 gaagcgctgc gcgatcgagc gctcgaactc ggagaccgcg gtggtggtgt cgccgcgctc
     3061 gagcgcgtcg atgccgaggt tgtagtgctg aatagcggcg tcgcggcagc ccgccagggc
     3121 cgctatcgcg agcagtagaa ttggaaacac acgatggcgc atggtcgacg ggcgggatga
     3181 tagccgccgg tcgtcgtgcg tgtcaaaccg agggaatccg aagggcgcgc ctagcgcgcg
     3241 taggggaagt cgggctggcc gccggccgtg cggagcttca cgcgcttggt ggcaccgtgc
     3301 acgatgaaga ccttctcgtt gttgctgtcg gggtcgcccg ggaagacgaa gaacccgaca
     3361 ccccgcgacg agtagctcag cgtgtagccg aagagaacct cgccgtcgag gaactcgacc
     3421 atcaccttct tgccgtgcgg gtaggtggag aagaaccctt tcttctcgtg gtagccggag
     3481 tcgccttcga agtcgcgcac gaagaacaac gccttcaagt cgccgacgtc gatctcgcgc
     3541 gtgccgtggc cgtcgtcgac gtggaagctg gtcttggtgg gcgcgaagtc gaaggtggtt
     3601 ccgcgcacca gcgtcccgtc caaataacga gcgatgaccc ggttgtcgag tttggggggc
     3661 atgacgcccg gagaactgca aggcgcatac cggcgcc
//
LOCUS       DASPGK010000242        11468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_297991, whole genome shotgun sequence.
ACCESSION   DASPGK010000242 DASPGK010000000
VERSION     DASPGK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297991"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VEC56_06585"
     CDS             complement(<1..943)
                     /locus_tag="VEC56_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXV13856.1"
                     /translation="MIHSVIVRAAKGRVTEDAVVFPVYSETKRLSALAARVDRSLGGA
                     AAAVLSSGSFRGERGETRIVTVKRGRRWAHAVVVGLGARAKANPEETADALGSAARAL
                     AALRIGSTALVVDQALVKEAPIPVELFVNAAVKGFVLATHDASLGAGSASTLRRLSIV
                     SDLPTRGTGGIARRAHDLCKLTARVRDWVNTPANQMTPTRLAAECRKLCAEHDVACRV
                     WSRTEIERARMGAVLAVASGSREQPRFVVAHYNVEKKSLPLVCLVGKGVTFDSGGVSI
                     KPWEKMHEMKGDMAGGAAVIAATAAAAALALPVRVVAL"
     gene            complement(966..1754)
                     /locus_tag="VEC56_06590"
     CDS             complement(966..1754)
                     /locus_tag="VEC56_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13857.1"
                     /translation="MRLSARWVALALCVVELGAVAAASAEPIIKPRKYHGPIPQSMAW
                     LRVGFMGDASNEEMNTYLDGLIQPPFTHDTEDFGTALVFDAGYARKPHPQFAFRLNAS
                     MAFLSSTSTGNKPTDDADSLLTVVDYSRDFKSDLIVLEASGMYFFSDAAVKEFQSYIG
                     GGFSLGIPHESFEETQIDIDTGEVFRTIETSEWGFSAGVHAVLGAIYYLTNTWGITTE
                     GRLQLMEGKYDQLQAPDENGVPEDVSFVIDYSGFYVTVGVLWGF"
     gene            1934..2422
                     /locus_tag="VEC56_06595"
     CDS             1934..2422
                     /locus_tag="VEC56_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HXV13858.1"
                     /translation="MKRILFVCSGNTCRSPLAEAIAGRMLPARVGFDVEVASAGTSAL
                     EGSPASTLSVEVASAHGLDLSSHRARIVDRAMVREADLIVTMGVRHRDTVGALDPDAL
                     EYTFLLTNFSDHRHGEIPDPIGSGRDTYERTYLAIRECIESMAGRLERFDGWKRVSGG
                     KS"
     gene            2419..2868
                     /gene="rpiB"
                     /locus_tag="VEC56_06600"
     CDS             2419..2868
                     /gene="rpiB"
                     /locus_tag="VEC56_06600"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXV13859.1"
                     /translation="MRIAVGADHRGFRLKELIKKRLRELGHDVVDVGTDGEASVDYTD
                     FAIAAAEKVARGEADRGVLVCGSGAGMSIAANKVKGVRAALAVSPDGARLAREHNDAN
                     VLALGAWQGSDERQVLQIAEAFLSAEFEGGRHARRVDKIRAYEENQS"
     gene            2877..4121
                     /gene="glyA"
                     /locus_tag="VEC56_06605"
     CDS             2877..4121
                     /gene="glyA"
                     /locus_tag="VEC56_06605"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXV13860.1"
                     /translation="MRSLEGVDRDVANVLRAEARRQHGTLEMIASENFVSPAVLAAMG
                     SVLTNKYAEGYPGKRYYGGCEEVDVAENLARDRARALFSAEHANVQAHSGSQANQIAY
                     FSFLKPGDTIMGLSLAHGGHLTHGHPVNFSGMFFKVVAYEVNRETKVLDYDALRALAL
                     RERPKLLVAGASAYARVWDFARLRSICDEAGCLLMVDMAHIAGLVAAGLHPSPVPHAD
                     VVTSTTHKTLRGPRGGFVLAPARNAEVVDKIAFPGMQGGPFMHIIAAKAVCFKEAASD
                     EFRAYQKQVVANARALAARFVERGFEVVSGGTDTHLFLLDLTAKDLSGKKAEAALEAA
                     GITVNKNTVPFDARSPFVTSGIRVGTPALTTRGMKEREMTRIADVMATVLDDPANEAT
                     IRAARAQTRDLCDAFPLYPELR"
     gene            4126..4596
                     /gene="nrdR"
                     /locus_tag="VEC56_06610"
     CDS             4126..4596
                     /gene="nrdR"
                     /locus_tag="VEC56_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXV13861.1"
                     /translation="MKCPRCGFEEDKVVDSRTTKEGEAIRRRRECLKCAFRFTTYEYI
                     ERAPLMVVKKDGRREQYSREKLLTGLLKACEKRPVSREQLEKIVDEIEATILGKFKNE
                     VKSTELGNLVIERLQALDEIAYVRFASVYRQFKDINQFMSEVRGLLDRQKDRPD"
     gene            4625..5449
                     /locus_tag="VEC56_06615"
     CDS             4625..5449
                     /locus_tag="VEC56_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13862.1"
                     /translation="MKRAVRFAVLGPMFASILASAPAVAQEVTFAYKFEPGTSERYRV
                     KLNQEVSMGQMEVSNLADMEVTVKCVSVADGKYAMELKFDKSDVSTTMMGNTSASPLG
                     EQLAGQTISFTADASGGVTDVKPVGVFEEWAMAQQLVEPVLQGWYPHLPNKAMAVGGE
                     WKQAGEKQVGAGGSESVTNASFKFKAMKKEKTRDVAVVEQTLDTTIGGTSATPMGVYS
                     VAGSGTGKGEFLFDPSKSRVVKVKGKVDLNMDMTPQSGGEAMNMIVANHFERELLE"
     gene            5460..6203
                     /gene="tatC"
                     /locus_tag="VEC56_06620"
     CDS             5460..6203
                     /gene="tatC"
                     /locus_tag="VEC56_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXV13863.1"
                     /translation="MRPFRSPAERTEILREMSFLEHLGELRGVLVASTIAFLGLSVVY
                     WFFSGTILDWVVARVPLDHLVFYAPSEAFMVRTKVAFVLGGMTAFPYIAYRLWRFVTP
                     GLFTREKSRVGPVVAASAVLFYGGVTFCYFVVAPAIINFMLGYATDRVQPMLSVSAYF
                     NTVARLCLAFGLVFQLPIVVLLLAVTGLVSPRTFLRQWRYAVVIIFVAAAIFTPPDPV
                     SQVLMAVPLVVLYIGSALVASALVRRRTA"
     gene            6214..6645
                     /gene="rsfS"
                     /locus_tag="VEC56_06625"
     CDS             6214..6645
                     /gene="rsfS"
                     /locus_tag="VEC56_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXV13864.1"
                     /translation="MTKRTAPATRTRKTPRTAPARAARATKSTAKKIAGYALEKKASD
                     VLVMDLRTVTDVTGFFIVCTGGSNAQVKAIADNVLEKCRQSGVGVYHVEGYDSLRWVL
                     IDLVDVVVHVFQPEVRSYYQLERLWGDAPSERLGEAEAAAR"
     gene            6670..8316
                     /gene="argS"
                     /locus_tag="VEC56_06630"
     CDS             6670..8316
                     /gene="argS"
                     /locus_tag="VEC56_06630"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXV13865.1"
                     /translation="MKEQIVAAIADALRRLGLDSEGVAMVVERPREAAHGDLSTPVAM
                     ALAKRLKQNPFAIAERLAGAIQLPGDFLAGVEAVRPDFVNFRLALPVLQGNVATVLAE
                     GERYGSSRDGEGKRVQVEYVSANPTGPLVIVSARAASIGNCLLHLFDACGYGTQGEYY
                     VNDSGKQIEALGESLRFRAKERLGATSPGETIEQYQYPGGYLATIASAADEKTARSWL
                     EPGASGAALGRYASQRLLDSIRDDMGAFGARFDRYFLESSLHPEALRVTFDRLVANGH
                     TYQQDGAVFFRSTAFGDDKDRVIRKADGEPTYFLADIAYHADKLARGFDRAIGLLGPD
                     HHGHVARMQAAMAALGAPADWLEIIIVGWVRLMEGGNPVSMSKRAGEFVTMRELIEDV
                     GVDVAKYFFLMRRANSPLDFDLTLARKQSDENPVYYVQYAHARIASVVRFAEERGDVG
                     APGRADASKLVEAEERALMLHLLHFPGTVAGAAEAREPHRVTTYAQELATAFHQFYHA
                     CRIVGEDPALSAARLDLARATKVVLKNALGLLGVGAPSSM"
     gene            8333..9268
                     /locus_tag="VEC56_06635"
     CDS             8333..9268
                     /locus_tag="VEC56_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXV13866.1"
                     /translation="MAEHRSSKETARPVLIVGSVAFDSVETPHGRADMALGGSASYAG
                     IAASYFAQPRVVGIVGDDFEDHHMRKLSDHGVDVRGIERAEGKTFHWKGRYHENMKDR
                     DTLETSLNVFQHFDPVIPEAYRRTPYVLLGNIDPALQLSVLDQAGAGAQFVAMDTMNF
                     WINTARDPLVEVLKRIDLLFINDEEAMQLADEWSVVAAAEKLLRMGPKFVVIKRGEHG
                     AILFGPKLTLFVPAVLLSNVVDPTGAGDTFAGGFMGQLSRTGSLDRTGLASALTWGTV
                     MASFTVEAFSVDGICGLAADAIADRRKTLVEMVRY"
     gene            9487..10197
                     /locus_tag="VEC56_06640"
     CDS             9487..10197
                     /locus_tag="VEC56_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13867.1"
                     /translation="MASHRLTVEQARRAEARVASVQGVSSCRIRTDESGQVTEVHVVS
                     RAGKSPKLVARDVEGVLKAEMDLDVDYKKIGVVVLDADHQAPADNPPEEFPILEHASR
                     FAFVSVNVVSTRAGLRAEVELSRDSAEAFGSSESENPSATALSVVAEATLRAVSEFLD
                     DNTRLCLGGVLKVSLGGGDAIVVRVDIVGARCNKSLAGSAMVAGNDAQAVVFATLDAI
                     NRLTGKLEFKSSIEYKIK"
     gene            10202..>11468
                     /locus_tag="VEC56_06645"
     CDS             10202..>11468
                     /locus_tag="VEC56_06645"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXV13868.1"
                     /translation="MKGGFEDSSLAFKLYYFGFIVLGFLFFAYLVRDFPLPRFPEVLL
                     FAFMIVVADTAQITLPRGGASIYASSPLDLAAIVLLGPSAAVLVEAIASFFSEVVVQR
                     RAAFKYAFNVPLLIMTIGVAGLAYWAFPGRWTALDTPLFLIPLTVCSVVYYLVNTFAI
                     SMVIALRGHRHVFHIWKQNYMWTFLHVLAFIPLGAIISLVYTAYGSWTLLLFLVPIFL
                     ARYTFKLYVEMKEAHINTVAALTSAIDASDPYTHGHSYRVSRYALRVGRALGLSARDV
                     EILEYGALLHDIGKIAIRHEILLKKERLSDQEFLTLKQHPTIGADIVENLKFLREAAV
                     LVRYHHEQPNGRGYPEGLKGDSIPMGARIILVCDAFDAMTSDRPYRKGLPVEHVVEQF
                     EKYKGEQFDLEIASLVVDLVRRGEFPLIVE"
BASE COUNT         1916 a         3738 c         3845 g         1969 t
ORIGIN      
        1 ccaacgccac cacgcgcacg ggcagcgcga gcgcggccgc ggcggcggtt gcggcgatca
       61 cggccgcccc gcccgccatg tcgcccttca tctcgtgcat cttctcccag ggcttgatgg
      121 agacgccgcc cgagtcgaac gtgacgccct tgccgaccag gcacaccagc ggcagcgact
      181 tcttctccac gttatagtgc gcgaccacga agcggggctg ttcgcggctg ccggatgcga
      241 ctgccagcac cgcgcccatg cgcgcgcgct cgatctccgt tcgcgaccac acgcggcatg
      301 ccacgtcgtg ttctgcgcac agcttgcggc attcggccgc cagccgggtc ggcgtcatct
      361 gattggccgg cgtattcacc cagtcgcgta cgcgcgcggt gagtttgcac agatcgtgcg
      421 cgcggcgcgc gatcccgccc gtgccgcggg tcggcaggtc ggacacgatg gaaagtcggc
      481 gcagggtgga ggccgagccc gcgccaagcg aggcgtcgtg ggtcgcgagg acgaacccct
      541 tgacggccgc gttcacgaac agctcgaccg ggatgggcgc ttccttcacc agagcctggt
      601 cgacgacgag ggccgtgctg ccgatacgca acgcggcgag cgcgcgcgcg gcggatccca
      661 gcgcgtcggc ggtttcttca gggttggctt tggcacgcgc gcccagcccg accacgaccg
      721 cgtgcgccca tcggcgcccg cgcttcacgg tgacgatgcg cgtctcgccc cgctcgccgc
      781 ggaacgagcc cgacgagagg accgcggccg cggcaccgcc caggctccgg tcgacgcgcg
      841 cggccagcgc cgagaggcgt ttggtttccg aatagacggg aaagacgacg gcgtcttcgg
      901 tgacgcgtcc cttggcggca cgcacgatca cggagtgaat catgctcgca tcgacgctcc
      961 cgcgtctaga agccccacag caccccgacc gtcacgtaga aacccgagta gtcgatcacg
     1021 aagctcacgt cttcgggcac cccgttctcg tcgggcgcct gcagctggtc gtacttgcct
     1081 tccatcaatt gcagacgtcc ttcggtcgtg atgccccaag tgttggtgag atagtagatc
     1141 gccccgagca cggcgtgcac gccggcggaa aacccccact cggacgtctc gatggtccgg
     1201 aagacttcgc cggtgtcgat gtcgatctgg gtctcctcga aggactcgtg cgggatgccg
     1261 aggctgaatc cgccgccgat gtacgactgg aactctttga cggcggcgtc ggagaagaag
     1321 tacatccccg aggcttcgag tacgatcagg tcggacttga agtcgcgcga atagtcgacg
     1381 acggtcagca gcgaatcggc gtcgtcggtg ggcttgttgc cggtgctggt cgagctcagg
     1441 aacgccatcg aggcgttcaa gcggaaggcg aactgcgggt gcggtttgcg cgcgtacccc
     1501 gcgtcgaaca cgagcgcggt gccgaagtcc tcggtgtcgt gggtgaacgg cggctggatg
     1561 agcccgtcga ggtaggtgtt catctcctcg ttcgacgcat cgcccatgaa cccgacgcgc
     1621 agccaagcca tcgattgcgg tatgggtccg tggtacttgc ggggctttat gatcggctcg
     1681 gcgctcgccg cggcgaccgc ccccaactcg accacgcaca gcgcgagcgc gacccatcgt
     1741 gcagatagtc tcaaggatgc ctcccaggcg aaaaagggcc ccgtaggttt ccctgctatt
     1801 agtcggaatc cgttgcaaag tcaacgggtt gtttgtatcg ttgcggtcgc ctcgcgcggc
     1861 gtcatggcgc ggttcgggaa cgcgccccga cccgggtgcg gccccaaggt atcccttccg
     1921 ggaaggccga ttcgtgaaga gaattctatt cgtctgctcc ggtaacacct gcaggagtcc
     1981 gctcgcggag gcgatcgccg gccggatgct tcccgcacgg gtgggcttcg acgtcgaagt
     2041 cgcttcggcc gggacctcgg ctctggaggg aagcccggcc tccacgctct cggtcgaggt
     2101 tgcgagtgcg cacgggctcg acctttcgtc gcaccgcgcg cgcatcgtcg accgggccat
     2161 ggtgcgcgag gccgacttga tcgtgaccat gggagtccgc catcgcgaca cggtgggagc
     2221 gctcgacccg gacgcgctgg agtacacctt cctcttgacc aatttctccg atcaccgcca
     2281 cggcgagatc cccgacccca tcgggtcggg gcgcgacacg tacgaacgta cgtatctcgc
     2341 catccgtgag tgcatcgagt cgatggcggg ccgcttggag cgcttcgacg gctggaagcg
     2401 cgtgtcggga ggcaagtcgt gaggatcgcc gtgggcgccg atcatcgagg attccggctg
     2461 aaggagctca tcaagaagcg cctgcgcgag ctcggccacg acgtcgtcga cgtcggtacc
     2521 gacggcgagg ctagcgtcga ctataccgac ttcgcgatcg cggccgccga gaaggtcgcg
     2581 cgcggcgagg ccgaccgggg cgtcttggtg tgcgggagcg gcgcgggcat gagtatcgcg
     2641 gccaacaagg tcaagggcgt gcgcgccgca ctcgcggtct cgcccgacgg cgcgcgactc
     2701 gcgcgcgagc acaacgacgc caacgtgctg gcgctgggcg cctggcaggg aagcgacgag
     2761 cgccaggtct tgcagatcgc ggaggcgttt ctgagcgcgg agttcgaggg cggacgccac
     2821 gcgcgccgcg tcgacaagat tcgcgcttac gaggagaacc agtcgtgacc gacctcatgc
     2881 gttcgctcga aggcgtcgac cgcgacgtcg ccaacgtgct gcgcgcggaa gcgcgccgcc
     2941 agcacggcac gctcgagatg atcgcgtcgg agaacttcgt gagcccggcg gtgctggccg
     3001 cgatgggctc ggtgctgacc aacaagtacg cggagggtta tccgggcaag cgctactacg
     3061 gaggctgcga ggaagtcgac gtggccgaga acctcgcgcg cgaccgcgcc cgggcgctct
     3121 tctccgccga gcacgccaac gtgcaggcgc actcgggcag ccaggccaat cagatcgcgt
     3181 acttctcgtt cctgaagccg ggcgacacca tcatggggct ctcgctcgcg cacggcggtc
     3241 acttgaccca cgggcatccc gtgaacttct cgggcatgtt cttcaaggtg gtcgcgtacg
     3301 aagtgaatcg cgagaccaag gtgctcgact acgacgccct gcgcgcgctg gcgctgcgcg
     3361 agcgccccaa gttgctcgtg gccggcgcca gcgcgtacgc gcgcgtgtgg gacttcgcgc
     3421 gcttgcgctc gatctgcgac gaagctggct gtctcctcat ggtcgacatg gcgcacatcg
     3481 cgggcttggt ggccgcgggt ctccacccca gcccggtccc gcacgccgac gtggttacca
     3541 gcacgaccca caagacgctg cgcggtccgc ggggcggatt cgtgctcgct cccgcgcgca
     3601 acgccgaagt cgtggacaag atcgctttcc ccggtatgca gggcggcccc ttcatgcaca
     3661 tcatcgcggc caaggcggtc tgcttcaagg aggcggcgag cgacgagttt cgcgcttacc
     3721 agaagcaagt ggtcgcgaac gcgcgcgccc tcgcggcccg cttcgtcgag cgcggcttcg
     3781 aggttgtgag cgggggcaca gacacgcatt tgttcttgtt ggatcttacc gccaaggatc
     3841 tttcgggcaa gaaggcggag gcggcgctgg aagcagccgg catcaccgtc aacaagaaca
     3901 cggttccctt cgacgcccgc agccccttcg tgaccagcgg catccgcgtg ggcacgcccg
     3961 cgctcaccac gcgcggcatg aaggagcgcg agatgacgcg catcgcggac gtgatggcga
     4021 cggtgctcga cgaccccgcc aacgaagcga ccattcgcgc cgcgcgcgcg cagacgcgcg
     4081 atctgtgcga cgccttcccg ctctacccgg agctgcggtg acgcgatgaa atgcccgcgc
     4141 tgcggcttcg aggaggacaa ggtggtcgac tcccgcacca ccaaggaagg cgaggccatc
     4201 cggcgccggc gcgagtgctt gaagtgcgcg tttcgcttca ccacctacga gtacatcgag
     4261 cgcgcgcccc tgatggtggt caagaaggac ggacggcgcg agcagtacag ccgcgagaag
     4321 ctcctgaccg ggttgctgaa ggcgtgcgag aaacggcccg tctcgcgcga gcagctggag
     4381 aagatcgtgg acgagatcga ggccacgatc ttgggcaagt tcaagaacga ggtgaagagc
     4441 accgagctcg gcaacctcgt catcgagcga ttgcaggcgt tggacgagat cgcctacgtg
     4501 cgcttcgcga gcgtgtaccg gcagttcaag gacatcaatc agttcatgag tgaagtgcgg
     4561 gggctcctcg accgtcaaaa agaccgcccc gattgaccta ccggggcgcg gaggtgattc
     4621 ggcgatgaag cgtgcagtga gattcgcggt gctcggcccg atgttcgcgt cgattctggc
     4681 gagcgcaccc gccgtcgccc aagaggtgac gtttgcgtac aagttcgagc ctggaaccag
     4741 cgagcgctac cgcgtgaagc tcaaccagga agtttccatg ggccaaatgg aggtatccaa
     4801 cctcgccgac atggaagtaa cggtcaagtg cgtgtccgtg gccgatggca agtacgccat
     4861 ggaattgaag ttcgacaagt ccgacgtgtc gaccaccatg atggggaaca cgtcggccag
     4921 cccgctgggc gagcagttgg ccgggcagac catctcgttc actgcggatg cgagcggtgg
     4981 tgtgaccgac gtcaagccgg tcggggtgtt cgaggagtgg gcgatggcgc agcagctggt
     5041 ggagccggtg ttgcagggat ggtacccgca cctgcccaac aaggcgatgg cggtgggcgg
     5101 cgaatggaag caggcgggcg agaagcaggt cggcgcgggc ggcagcgaga gcgtgaccaa
     5161 cgcgagcttc aagttcaagg cgatgaagaa agagaagact cgcgacgtcg ccgtcgtcga
     5221 gcaaacgctc gacactacca tcggcgggac cagcgcgacg ccgatgggcg tctacagtgt
     5281 cgcggggtcc ggcacgggca agggagagtt cctattcgat ccgagcaagt cgcgcgtcgt
     5341 gaaggtcaaa ggcaaggtcg atctcaacat ggacatgacg ccgcaatcgg gcggcgaggc
     5401 gatgaacatg atcgtcgcca atcacttcga gagggaactc ctcgaataga gatccgcaga
     5461 tgcgcccgtt ccgctctccc gcggaacgaa ccgagatcct gcgcgagatg tcgttcctcg
     5521 agcacctcgg cgaattgcgc ggggtgctcg tcgcgtcgac gatcgcgttc ctcgggctct
     5581 cggtggtcta ctggttcttc agcgggacca tcctcgactg ggtcgtcgcg cgcgtcccgc
     5641 tggaccacct cgtcttctac gccccctcgg aggcgttcat ggtgaggacg aaggtcgcct
     5701 tcgtcctggg cggcatgacc gccttcccgt acatcgcata tcgtctatgg cgcttcgtca
     5761 cgcccgggct gttcacgcgc gagaagagcc gagtgggtcc ggtcgtggcc gcctccgcag
     5821 tgctgttcta cggcggcgtt accttctgct acttcgtggt cgcgccggcg atcatcaact
     5881 tcatgctggg ctacgcgacc gatcgtgtgc agcccatgct ctcggtttcg gcgtatttca
     5941 acaccgtcgc gcggctgtgc ctcgcgttcg ggttggtgtt ccaactgccg atcgtggttc
     6001 tccttctcgc cgtcacggga ctggtgtcgc cgcgcacgtt cctgcgccag tggcgctacg
     6061 ccgtggtcat catcttcgtg gcggccgcga tcttcacccc gcccgacccg gtatcgcagg
     6121 tactgatggc agttcccctc gtggtcctgt atattgggag cgctttggtg gcgtcggcgc
     6181 tggtgcgccg gcgcactgcg tgacggagga cgcgtgacga agcgaaccgc cccagcaaca
     6241 aggacccgca agaccccgag aaccgccccg gcccgcgccg cccgggccac caagtcgacc
     6301 gcgaagaaga tcgccggcta cgccctcgag aagaaagcga gcgacgtgct ggtgatggac
     6361 ctgcgcacgg tcaccgacgt gaccggcttc ttcatcgttt gcacgggggg atcgaacgcg
     6421 caggtcaagg ccatcgccga caacgtgctc gagaagtgcc ggcagtcggg cgtgggcgtc
     6481 tatcacgtgg aaggatacga ctcgttgcgc tgggtcttga tcgacctggt cgacgtggtg
     6541 gtgcacgtgt tccagcccga agttcgttcg tactaccaac tcgagcgcct gtggggcgac
     6601 gcgcccagcg aacgcctggg cgaggccgaa gccgccgctc gctgatccct cgcctcgata
     6661 ctcatgggcg tgaaggaaca aatcgtcgcc gcgatcgccg acgcgctgcg tcgtctcggc
     6721 ttggactccg aaggcgtcgc gatggtcgtg gagcgcccgc gcgaggccgc gcacggggac
     6781 ctctcgaccc ccgtggcgat ggcgctcgcg aagcgcctca agcagaaccc gttcgccatt
     6841 gccgagcgcc tggccggcgc catccagctc ccgggtgatt ttctcgccgg cgtggaagcg
     6901 gtgcgccccg acttcgtcaa tttccggctg gcgctgccgg tgctgcaagg caacgtggcc
     6961 acagtgctgg ccgagggcga gcgctacggc agctcgcgtg acggcgaagg caagcgcgta
     7021 caagtcgagt acgtgtccgc caacccgacc ggccccttgg tgatcgtgtc cgcgcgcgcg
     7081 gcctcgatcg gcaactgcct gttgcatctt ttcgacgctt gcggctacgg cacgcaaggc
     7141 gagtactacg tcaatgactc cggcaagcag atcgaggcat tgggcgagag cctgcgtttt
     7201 cgcgccaagg aacgcctggg agcgacctcg ccgggcgaaa ccatcgagca gtaccagtac
     7261 cccggcggtt acctcgccac catcgcgtcc gcggccgacg agaagaccgc gcgctcgtgg
     7321 ctcgagcccg gcgcgagcgg cgccgcgctg gggcgctacg cgtcgcagcg cctgctcgat
     7381 tcgattcgcg acgacatggg ggcctttggc gcgcgcttcg atcgctactt cttggagtcg
     7441 tcgctgcacc ccgaggcctt gcgcgtcacc ttcgatcgct tggtcgccaa cggtcacacc
     7501 taccaacagg acggcgcggt gttctttcgc tcgaccgcgt tcggcgacga caaggaccgc
     7561 gtgattcgca aggccgacgg cgagccgacg tatttcctgg ccgacatcgc ctaccacgcc
     7621 gacaagcttg cgcgcggctt cgatcgcgcc atcgggctgt tgggccccga ccaccacgga
     7681 cacgtcgcgc gcatgcaggc cgcgatggcg gcgctgggcg cgcccgccga ctggctggag
     7741 atcatcatcg tgggctgggt gcgcctgatg gaagggggca accccgtcag catgagcaag
     7801 cgcgcgggcg agttcgtcac catgcgcgag ttgatcgagg acgtcggggt cgacgtggcg
     7861 aagtacttct tcttgatgcg acgggccaat tcgccgctcg acttcgatct gaccctggcg
     7921 cgcaagcagt cggacgagaa ccccgtctac tacgtgcagt acgcgcacgc gcgcatcgcg
     7981 agtgtggtgc gcttcgctga ggagcggggc gacgtggggg cccccgggcg cgccgacgcc
     8041 tccaagctgg tcgaagccga ggagcgcgcg ctcatgctcc acctgttgca cttcccgggc
     8101 acggtggcgg gggcggcgga agcgcgcgag ccgcaccggg tcacgaccta cgcccaggag
     8161 ctcgcgaccg cgttccacca gttttatcac gcgtgccgaa tcgtgggcga agaccccgcg
     8221 ctgtccgcgg cgcgcctgga tctcgcgcgc gcgaccaagg tcgtcctcaa gaacgcgctg
     8281 ggattgctgg gcgtgggcgc gcccagcagc atgtagcaag gagtcgaagt cgatggccga
     8341 acatcgttcg agtaaggaaa cggcccggcc ggtcttgatc gtggggtcgg tggcgttcga
     8401 ttcggtggaa acaccgcacg gacgcgcgga catggcgctg ggaggatccg cgagctacgc
     8461 cggcatcgcc gccagctatt tcgcgcagcc gcgcgtggtc ggaatcgtcg gcgacgactt
     8521 cgaggaccac cacatgcgca aactctccga ccacggcgtc gacgtgcgcg gcatcgagcg
     8581 ggcggaaggg aagaccttcc actggaaagg ccgctaccac gaaaacatga aggaccgcga
     8641 tacgctggag acctctctca acgtcttcca gcacttcgat ccggtgatcc cggaggcgta
     8701 tcggcgcacg ccctacgtcc tcttgggcaa catcgacccc gcgttgcagc tctccgtgct
     8761 cgaccaggcc ggggccggcg cgcagttcgt cgccatggac accatgaatt tctggatcaa
     8821 caccgcgcgc gacccgctgg tcgaagtgct caagcgcatc gacctcctgt tcatcaacga
     8881 cgaggaagcg atgcagctcg ccgacgagtg gtcggtggtg gcggcggcgg agaagctcct
     8941 gcgcatgggc ccgaagttcg tcgtgatcaa acgcggcgaa cacggcgcga ttctcttcgg
     9001 ccccaagctg acgctgttcg tccccgcggt gctcctgtcc aacgtggtcg acccgaccgg
     9061 cgccggcgac accttcgcgg gcggtttcat gggccagctc tcgcgtacgg gcagcctcga
     9121 tcggaccgga ctcgcgtccg cgctcacgtg gggcacggtc atggcgagct tcacggtcga
     9181 agcgttctcg gtcgacggca tctgcggcct cgccgcggac gcgatcgccg accggcgcaa
     9241 gacgctggtc gagatggtcc gctactagct cacggcgccg ccggtcgagg cgatcgatcc
     9301 ctcgacgcga tttctgcagc gcgaatacca ccccatattg tctgcctcgc gctgcagttt
     9361 gagcggagag ggacgatcgc atcggccgga gcgcgatgct ggtcgggttc ttgcgtcatt
     9421 ccggtgtggt ataatcacgg tcgccggcgc ccccaccggt ttgggcgcgg ggcgacgtta
     9481 taacgtatgg cctcgcatag acttacagta gaacaggcgc gccgcgccga ggcccgcgtc
     9541 gcgagcgtgc agggtgtgtc ctcgtgccgg attcgcaccg acgaatccgg gcaagtgacc
     9601 gaggtccacg tcgtctcgcg cgcgggtaag tcgcccaaac tcgtggcgcg cgacgtcgaa
     9661 ggagtgctca aggccgagat ggacctcgac gtcgactaca agaagatcgg cgtcgtcgtg
     9721 ctcgacgccg accaccaggc acccgccgac aatccacccg aagagtttcc catcctcgag
     9781 cacgcgtcgc gcttcgcgtt cgtcagcgtg aacgtggtgt cgacgcgcgc cgggctgcgc
     9841 gccgaagtcg agctgtcgcg cgattcggcg gaggcgttcg ggtcgagcga atccgaaaat
     9901 ccctcggcga ccgcgctgtc ggtggtggcg gaggccacgc tgcgcgccgt ctcggaattc
     9961 cttgacgaca acactcgcct gtgtttaggt ggcgtgctga aggtgtcgct gggcggcggc
    10021 gacgcgatcg tggtgcgcgt cgacatcgtc ggtgctcgct gcaacaagag tctcgcgggt
    10081 tcggcgatgg tcgccggcaa cgacgcccag gcggtcgtct tcgcgacgct cgacgccatc
    10141 aatcgtttga ccgggaagct ggaattcaag agttcgatcg aatacaagat caagtgaccc
    10201 cttgaaaggc ggcttcgaag actcttcgct cgcgttcaag ctctactact tcgggttcat
    10261 cgtcctcggt ttcctgttct tcgcttatct cgtccgcgat tttccgctcc cgcgctttcc
    10321 cgaagtcctg ctgttcgcct tcatgatcgt cgtggccgac acggcccaga tcacgcttcc
    10381 gcgcgggggt gcgtccatct acgcgtcgag cccgctggac ctggccgcca tcgtgctcct
    10441 gggcccgtcg gcggcggtgc tggtcgaagc catcgcgtcc ttcttctcgg aagtggtggt
    10501 gcagcggcgc gcggccttca agtacgcctt caacgtaccg ctcctcatca tgaccatcgg
    10561 agtcgcgggg ctcgcctact gggcgtttcc gggtcgctgg accgcgctcg acacgccgct
    10621 cttcttgata ccgctgacgg tttgcagcgt ggtctattac ctcgtcaata ccttcgcgat
    10681 cagcatggtg atcgcactgc gcggccaccg gcacgtgttc cacatctgga agcagaacta
    10741 catgtggacc ttcctgcacg tgctcgcgtt catcccgctg ggcgccatca tttcgctggt
    10801 gtacacggcg tacgggagtt ggacgctgct cttgttcctg gttcccatct tccttgcgcg
    10861 ctataccttc aaactctatg tcgagatgaa ggaggcgcat atcaacacgg tggcggcgtt
    10921 gacgtccgcc atcgacgcca gcgacccgta cacgcacggc cactcgtacc gcgtctcccg
    10981 ctatgcgctg cgcgtcggcc gcgcgctggg cctctcggcg cgcgacgtcg agatcctcga
    11041 gtacggggcg ctcctgcacg acataggcaa gatcgcgatc cgccacgaga ttctcttgaa
    11101 gaaggagcgc ttgagcgacc aggagttcct gaccttgaag cagcacccga ccatcggcgc
    11161 cgacattgtc gagaacctca agtttctgcg ggaagccgcc gtcctggtgc gctatcatca
    11221 cgagcaaccg aacgggcgcg gctatccgga aggattgaag ggcgactcga tcccgatggg
    11281 cgcgaggatc attctcgtgt gcgacgcctt cgacgccatg acgtccgatc ggccgtaccg
    11341 caagggcttg ccggtcgagc acgtcgtcga acagttcgag aagtacaagg gcgagcagtt
    11401 cgacctggag atcgcgagcc tggtggtcga cctcgtgcgg cgcggggagt tcccgctcat
    11461 cgtcgaga
//
LOCUS       DASPGK010000243         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_62346, whole genome shotgun sequence.
ACCESSION   DASPGK010000243 DASPGK010000000
VERSION     DASPGK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62346"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1640
                     /locus_tag="VEC56_06650"
     CDS             312..1640
                     /locus_tag="VEC56_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13869.1"
                     /translation="MKRKGKVLLDRISSAPRGLEDAVQFPLIEALHGRRARRFSLGAA
                     IPDGPLAFTSTHPPMPLDDLEQMMVLTAVAGNTGWFYLHPYNKHYIPNIPNYAVGAGG
                     RTFPSAAGFHTSEIFFTDDSGVYFFPTRDAPDLTERNADGAIDLNAYFAAHRSRVKKL
                     SDRRLHIPDNPAHIDQHNPWCVNRPGSTLVIPVADLAQHYLAALCYIVQNGACMFDDI
                     NHAKIPGMERFASIVDVNNPYPLTYMEQLCLAEVTAELSTACYAGMLMLQATGLGGWM
                     FDGLTPFSILGASGDPEMPGLGFRYDTNPRWSIPNATGLSGVFEGFCPPHYKDMRAAT
                     EAIVQRKFGPGGPFNPQTAGPYRENARVRGAGKVHDDQFKECVATMAQYVFDRFGKFP
                     GTVPSMFALTYLQAHHLDLEFYDQHFAPGAYLETHAHHMERWHGAKQAGT"
     gene            1647..2324
                     /locus_tag="VEC56_06655"
     CDS             1647..2324
                     /locus_tag="VEC56_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13870.1"
                     /translation="MPAYAIVALGVVVVFFSITLGVRANRAKQEARRMELQRLGFVPC
                     DDKKDWLQKTVAAIENNKGYEYEVRNAYRLTGSAEVYYYHKRRDGPKTDDAFGEEEVL
                     FPLKRPSSQGLLLAVKPSALGHGRATRLIGAVATGPWDSQPDDLERIDIPAELAKTNL
                     LGALGPAGARLYDLVDDRTVSALQRLGDAGAISIHFRGEWCTVSSGGTKPPLHLGDVL
                     ASIRPLL"
     gene            2447..>3339
                     /locus_tag="VEC56_06660"
     CDS             2447..>3339
                     /locus_tag="VEC56_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13871.1"
                     /translation="MTIRSLRFFVALTTICSTWASLASAQGANIGLYTDASGTTCSFT
                     GDGAGMVTAYVVFRPDINGVNGVQFAAPVPTCFGATFLNDVAPPGVLKIGSSQTGVSV
                     ALPGCYGQPVNVLQINFQRIGGATTACCEFPIVADPSVGSILVSDCTYQNIPVTVVSS
                     RFNADATCACVGNSAPGLPSFVQPVDGSSGVTVAPQMAWSANDWDNNITEHDVYLGTS
                     STPPLAASGLPQASYTPGALQELTQYFWRVVVRDAFGLETASPTWTFTTRLANTPPIA
                     PYGPSPIDGAINVLLDVPLDW"
BASE COUNT          580 a         1137 c         1059 g          563 t
ORIGIN      
        1 accgaagact acgagggaag cgaatgagag gcggcgcatg gcaaccctcc tcggggttcg
       61 agtgccgcct cggcgggtgc cgtgtcgtgc aagaggtgcg ggcggccgca atggaggttt
      121 cgttaagggt ttatgatacc atatcgccca accccgagga ctcggcaata cggtttcccg
      181 ctggaacgag cgtttcgacc ggtggtagca tgccggccat gcaactcaca cgacggtccc
      241 tactcggtta tctcctcgtc ggcaccgggg cgctcctcgc cggctgcgga ttgaattcgc
      301 aaggcgagcg catgaagcga aaggggaagg tactcctcga tcgtatctcg tcggcgccgc
      361 gcggactcga agacgccgtc cagtttccct tgatcgaagc gctccacggc cggcgcgcgc
      421 gacgcttctc gctgggggct gcgatccccg acggcccgct cgcattcacg tccacgcacc
      481 cgcccatgcc gctcgacgac ctcgagcaga tgatggtgct gacggcggtc gcggggaaca
      541 cgggctggtt ctacctccat ccgtacaaca agcactacat ccccaacatt cccaactacg
      601 cggtgggcgc gggcgggcgc acgtttccgt cggccgcggg ttttcataca agcgagatct
      661 tcttcaccga cgactcgggt gtgtacttct ttccgacgcg cgacgcgccc gatctgaccg
      721 agcgcaacgc cgacggcgcg atcgatctca acgcgtactt cgcagcccac cgttcacgcg
      781 tgaagaagct ctccgaccgg cgccttcaca ttcccgataa cccggcccac atcgaccagc
      841 acaatccgtg gtgtgtcaat cgcccgggtt ccacactcgt gattccggtg gcggacttgg
      901 cgcagcacta cctcgcggcc ttgtgctaca tcgtccagaa cggcgcgtgc atgttcgacg
      961 atatcaacca tgcgaagatc ccgggtatgg agcgttttgc gagcatcgtc gacgtgaaca
     1021 acccgtatcc gctgacgtac atggaacagc tttgtctcgc ggaggtgacc gcggagctct
     1081 cgactgcgtg ctacgcgggg atgttgatgc tgcaggcgac ggggctgggg ggctggatgt
     1141 tcgacgggct aacacccttc agcatcctcg gcgcgagcgg cgaccccgag atgccgggcc
     1201 tgggatttcg ctacgacacc aacccgcgct ggtcgattcc caacgcgacc gggctgtcgg
     1261 gcgtgttcga gggcttctgc ccgcctcatt acaaggacat gcgcgccgcc accgaggcca
     1321 tcgtgcagcg caagttcggt ccgggcgggc cgttcaaccc gcagaccgcg gggccttacc
     1381 gagagaacgc gcgcgtgcgc ggcgccggga aggtccacga cgaccagttc aaggaatgtg
     1441 tcgccaccat ggcgcagtac gtcttcgatc gcttcgggaa gttcccgggc accgtgccgt
     1501 cgatgttcgc gctcacctac ttgcaggcgc accacctcga cctcgagttc tacgaccagc
     1561 acttcgcgcc gggggcgtat ctcgagacgc acgcacacca catggagcgc tggcacggcg
     1621 cgaagcaagc ggggacgtga gcgcgcatgc cggcgtacgc catcgtcgcc ttgggggtcg
     1681 tggtcgtgtt cttttcgatc acgctgggcg tgcgagcgaa ccgcgccaag caggaagcgc
     1741 gccggatgga gttgcagcgg ctcggtttcg tgccgtgcga cgacaagaaa gactggctgc
     1801 agaagaccgt cgccgcgatc gagaacaaca agggctacga gtacgaggtt cgtaacgcgt
     1861 accggctcac cggcagcgct gaagtctact actaccacaa gcgacgcgac ggcccgaaga
     1921 ccgacgacgc cttcggcgag gaagaggtct tgtttcccct gaagcgacct tcgtcgcaag
     1981 ggctgttgct cgcggtgaaa ccgtccgcac tcggtcacgg ccgtgccacg cgtttgatcg
     2041 gcgcggtcgc gaccggccca tgggactcgc agcccgacga cctcgagcgc atcgacatcc
     2101 ccgccgaact cgcgaagacc aatctgctcg gagcactcgg ccccgccgga gcgcgcctgt
     2161 acgatctcgt cgacgaccgc acggtgagcg cgctgcagcg gctgggcgac gcgggggcga
     2221 tatcgatcca ctttcgcggc gagtggtgca cggtgagcag cggcggcacc aaaccgccgc
     2281 tccacctggg cgacgtgctc gcatccatcc ggccgcttct gtaggcctcg tccggcgccc
     2341 gccggagcaa aaaaacattc cgtcgggtta gccgaacgcc cgttctttgg cgcacatacc
     2401 tgtggaagcg ggcaactcca gatgcccgcc cgatacccgc ggcgccatga cgattcgatc
     2461 tcttcgcttt ttcgtcgccc ttacgacgat ctgctcgacg tgggcgtcac tcgcctcggc
     2521 ccaaggcgcc aatatcggtc tctacaccga cgcctccggg acgacgtgca gcttcaccgg
     2581 cgacggagcc ggcatggtga ccgcgtacgt ggtcttccgc cccgacatca acggcgtcaa
     2641 cggcgtccag ttcgcggccc cggttccgac ttgcttcggc gccacgttcc tcaacgacgt
     2701 cgccccgccc ggcgtgctga agatcgggtc gtcgcagacg ggggtctcgg tggcgctacc
     2761 ggggtgctac ggacaaccgg tcaacgtgct gcagatcaac ttccagagga tcggcggtgc
     2821 gaccacggcg tgctgcgaat ttcccatcgt cgccgacccg tcggtgggga gcatcctcgt
     2881 cagcgactgc acgtaccaga acatccccgt cacggtggta tcgagccgct tcaacgccga
     2941 cgccacctgc gcgtgcgtgg gcaacagcgc gcccggactg ccatcatttg tccagcccgt
     3001 cgacgggagc tccggggtca cggtcgcgcc gcagatggcg tggtccgcca acgactggga
     3061 caacaacatc accgagcacg acgtctacct gggaacgagt tcgaccccgc ccttggcggc
     3121 atccgggctc ccgcaggcga gctacacccc gggcgcgctg caagagctga cgcagtactt
     3181 ctggcgcgtc gtcgtgcgcg acgccttcgg gctcgagacg gcgagcccga cctggacgtt
     3241 cacgacgcgc ctggcgaaca ccccgccgat cgcgccgtat gggcccagtc cgatcgacgg
     3301 ggcgatcaac gtgctcctcg acgtgccgct ggactggca
//
LOCUS       DASPGK010000244         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_648241, whole genome shotgun sequence.
ACCESSION   DASPGK010000244 DASPGK010000000
VERSION     DASPGK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648241"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..951)
                     /locus_tag="VEC56_06665"
     CDS             complement(79..951)
                     /locus_tag="VEC56_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXV13872.1"
                     /translation="MAEPQRQTPWTTELEGIRAPLGVILYLIRRDNLDIYDIPIAKIT
                     REYLDYLNLMEGMQIELAGEFFVLAATLMRIKIQMLLRKDDDTDEDPRQELVQSILEY
                     KKMVEAARTLKDLEDERMRVFTRPVPQEEKDATSGEVVIDLNLFQLMRAFQEVMTQFE
                     GAEVREVELEAYTIEEKIDLVRATLEAREPAMFADLFSGSRSRLEVIVTFMAILELLK
                     HGQARVEQEGSFGSIWIYKGENFGRPMGEVSDWSAEAEILEVTAPVESGGEDAFDDRA
                     GDIEGETDGESEPR"
     gene            complement(944..1921)
                     /gene="trpS"
                     /locus_tag="VEC56_06670"
     CDS             complement(944..1921)
                     /gene="trpS"
                     /locus_tag="VEC56_06670"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXV13873.1"
                     /translation="MLSGNRPTGRLHLGNYTGALENWVALQKEYRCYFMVADWHVLTT
                     DYEHTAQIDGNTHEMVLDWISAGLDPETSTIFVQSHVKEHAELYLLLAMLISTARLER
                     NPTLKEQVRDLKIDEGEEGMSYGHLGYPVLQAADILIYRAHVVPVGEDQLPHIEITRE
                     IARKFNRLYGEVFPVPDGKLTKFARFPGLDGRRMSKSLGNTIDLADSPEEIQKKISTA
                     FTDPQRKRRTDPGRPEVCLIYTYWQKFKPNEAAEIYEGCTTATLGCVECKQRVGRAIA
                     EYLSPLREKRKTLEARAGAVEEVIHAGDAKARRAAADTMAEVRERMHIG"
     gene            complement(2038..2940)
                     /gene="xerD"
                     /locus_tag="VEC56_06675"
     CDS             complement(2038..2940)
                     /gene="xerD"
                     /locus_tag="VEC56_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXV13874.1"
                     /translation="MKRSIDSSVDAFLDYLVVEKGLLPNTIHAYSRDLRSYIETLEEI
                     SVTDPARITDEAIELHAVRLSRRGLAPASRARKLSALRHFHKFLAREGWGSERVARDV
                     VRPKLARRLPSVLSLSQIESLIEQPDTTTPLGLRDRAMLELAYGAGLRVTELCGLTVE
                     ELKLDQEIVVVRGKGGKQRVVPFGRQAARALARYLDGGRSRLLAGRISPHVFVNARGG
                     GISRVGFFKNLKAHAASAGIARRVSPHVLRHSFATHLLEGGADLRLVQELLGHADIAT
                     TQVYTHVDTRHILEAHRTFHPRGR"
     gene            3027..3272
                     /gene="rpsT"
                     /locus_tag="VEC56_06680"
     CDS             3027..3272
                     /gene="rpsT"
                     /locus_tag="VEC56_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXV13875.1"
                     /translation="MPQHKSCEKRIKQDVKRTARNRAERGNLRTIVRAFRELDAGKRP
                     AEFSQLQSMLDRAVKKGIISKNRAARLKSRLAPKKAA"
BASE COUNT          598 a         1157 c         1156 g          677 t
ORIGIN      
        1 gtgacgtctt cgatcgtggc cgcgatcttg cgcgccgaga gcggttcgtc ggcggcgaag
       61 agaagcgcca ggacgatgtt atcgaggttc ggattctcca tcggtctctc cttcaatgtc
      121 ccccgcacga tcgtcgaacg catcctctcc gcccgactcc accggtgccg tcacttccaa
      181 gatctccgct tcggcgctcc agtcggagac ttcccccatc gggcgcccga agttctcgcc
      241 cttgtagatc cagatcgaac cgaacgagcc ttcctgttcg acccgcgcct gcccgtgctt
      301 caacagttcc agaatcgcca tgaaggtgac gatcacttcc aggcgcgagc gcgagccaga
      361 gaacaagtcg gcgaacatgg cgggctcgcg cgcctccagg gtggcacgca ccaggtcgat
      421 cttctcttcg atggtgtagg cctcgagctc gacctcgcgc acctccgcgc cctcgaactg
      481 ggtcatgacc tcctggaagg cgcgcatgag ctggaagaga ttcaagtcga tcacgacctc
      541 gccgctggtc gcgtcttttt cttcctgcgg gaccggtcgc gtgaagacgc gcatgcgctc
      601 gtcctccaga tccttcaagg tgcgggcggc ttcgaccatc ttcttgtact cgaggatgct
      661 ctgcaccagc tcctggcgcg ggtcctcgtc ggtgtcgtcg tccttgcgca agagcatctg
      721 gatcttgatg cgcatcaggg tcgcggccag cacgaagaac tcccccgcca gctcgatctg
      781 catgccctcc atgagattga gatagtcgag gtactcgcgc gtgatcttcg cgatcggaat
      841 gtcgtagatg tcgaggttgt cgcggcggat cagatacaga atcacgccca gcggcgcgcg
      901 aatcccctcg agctccgtgg tccacggggt ctgtctctgt ggttcagcca atgtgcatcc
      961 gctccctgac ttccgccatg gtatcggcgg ccgcgcggcg cgccttggcg tcgcctgcgt
     1021 ggatcacttc ttccaccgcg cccgcgcgcg cttccagcgt cttgcgcttc tcgcgcagcg
     1081 gcgagagata ctccgcgatg gcgcgcccta cgcgctgctt gcactcgacg cacccgagcg
     1141 tcgcggtggt gcagccttcg tagatctcgg ccgcttcgtt gggcttgaac ttctgccagt
     1201 aggtgtagat caagcatacc tcgggacggc ccgggtcggt gcgacgtttg cgttgcgggt
     1261 cggtgaaggc ggtcgatatc tttttctgaa tttcttccgg cgaatccgcg agatcgatgg
     1321 tgttgccgag cgacttgctc atacgacgcc cgtcgaggcc cgggaaacgc gcgaactttg
     1381 tgagcttgcc gtcgggcacc gggaagacct cgccgtagag ccggttgaac ttgcgcgcaa
     1441 tctcgcgcgt gatttcgatg tgcggcaatt ggtcctcacc caccgggacc acgtgcgcgc
     1501 ggtagatcag gatgtcggcc gcttgcagca ccgggtatcc caagtggccg tagctcatgc
     1561 cctcttcgcc ctcgtcgatc ttcaggtcgc gcacctgctc cttgagcgtc gggttgcgct
     1621 ccaggcgcgc ggtcgagatg agcatcgcga gcaggagata cagctccgcg tgttccttga
     1681 cgtgcgactg gacgaagatg gtcgacgtct cggggtccag tccggcgctg atccaatcca
     1741 gcaccatctc gtgggtgttg ccgtcgatct gggcggtgtg ctcgtagtcg gtggtcaaga
     1801 cgtgccagtc cgccaccatg aagtagcagc gatactcctt ctgcagggcg acccaattct
     1861 ccagcgcccc ggtgtagttg ccgaggtgca ggcgcccggt ggggcgattc ccgctcaaga
     1921 tgatcttcgt tgctgtctgg ggggccaccc gatgctccaa gccacgccgt gacacggcgt
     1981 tttgcgcatt ctaggcgggc gtttcgaggg gtgtcaagga atgtcggggc gggagtgtca
     2041 gcgtccccgc gggtggaacg tccggtgcgc ttccagaata tggcgcgtgt cgacgtgggt
     2101 gtagacctgg gtcgtcgcga tgtcggcgtg gcccagcagt tcctgcacca atcgcaggtc
     2161 cgccccgcct tccaggaggt gggtcgcgaa ggagtgccgc agcacgtgcg gggagacgcg
     2221 gcgcgcgatg ccggccgagg ccgcgtgggc cttgagattc ttgaagaacc ccacgcgcga
     2281 gatcccaccc ccgcgcgcgt tcacgaacac gtggggtgag atgcgccccg cgagcaagcg
     2341 cgaacggccc ccatcgaggt agcgcgcgag ggcacgcgcg gcctggcgcc cgaacgggac
     2401 cacgcgctgc ttgccgccct tgccgcgcac gacgacgatc tcctggtcca atttgagctc
     2461 ttcgacggtg agaccgcaca gctcggtcac gcgcagcccg gccccgtagg ccagctccag
     2521 catcgcgcga tcgcgcaacc ctaggggcgt cgtggtgtcg ggctgctcga tcaacgactc
     2581 gatttgtgag aggctcaata ccgatggcag ccggcgcgcg agcttgggcc gcacgacgtc
     2641 gcgcgcgacg cgttcgctgc cccacccctc gcgcgcgagg aacttgtgaa agtgccgaag
     2701 tgcggagagt ttgcgcgcgc gtgacgccgg cgccaggccc cgccgcgaga gccggaccgc
     2761 atgcagctcg atggcttcgt cggtgatacg cgcgggatcg gtgaccgaga tctcttccag
     2821 cgtctcgata taagagcgaa ggtcgcgcga gtaggcgtgg atggtattgg gcaggagccc
     2881 cttctcgacc acgaggtagt cgaggaaggc gtcgacggat gagtcgatgg accgtttcac
     2941 cgtgccaaac ccgctaaatc cagcccttga cgggcccccg gtaacctgat actttccttc
     3001 ggctcattat tcaggaggaa atctggatgc cacaacataa atcgtgcgag aagcggatca
     3061 agcaggacgt caagcgcacc gcccgcaacc gcgccgagcg cggcaacctg cggaccatcg
     3121 tgcgcgcgtt ccgcgaactc gacgccggca agcggccggc cgaattctcg cagctgcagt
     3181 cgatgctgga ccgcgcggtc aagaagggga tcatctccaa gaaccgtgcg gcccgcttga
     3241 agtcacgcct ggcccccaag aaggccgcgt aacgcccgtc gtcgcagcct cggactctcg
     3301 agcgccgtcc atcgaagaga tggacggcgt tttgcgttgt ggaggggcgc tggcaacgcc
     3361 ggccaacttt cgccccccgg gcctgtaatc caactagtaa actcgtgcgg tcgtaacaca
     3421 cccaacctgg cgacccgcat gggcgttcgc ccgcaagtgg cgtgtacttc gtgcggctgg
     3481 tcacagccgg cgaagaggtg gcgcgcaaga tggtggtttt gaagtagcgt tcactcgagc
     3541 ccgcgccata cgtagatggc cggccaaatc ggagggcaat cgcatgcg
//
LOCUS       DASPGK010000245         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_883412, whole genome shotgun sequence.
ACCESSION   DASPGK010000245 DASPGK010000000
VERSION     DASPGK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883412"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /gene="pstA"
                     /locus_tag="VEC56_06685"
     CDS             <1..1746
                     /gene="pstA"
                     /locus_tag="VEC56_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXV13876.1"
                     /translation="STPVASPNHSPPRKAPRARGGTSLLAQGENMLWLTGGALAVCSV
                     MIVGFLVLVLAQGTATFWPKPLVEVTLDDGRVLLGEVTRTERFTPTPETVAGWSEQAR
                     ARFQNDAAERGGRATRRLVRTGNFELTGTHFEWIDDVAVVRESRPDWATLLERRAWGR
                     FYGVPKEFRVDGNAVASTPSAAWREFRRHHDAVATRFERRYQLQKRDFGELHARAEGA
                     RLEAKEVEAKYGDQSPQHETALRMLEGEMRAIESQRRTLEEEVRLLDEENARYELVLQ
                     TTSGEDAAIPLADVVRAFPANQLSAASKLGVYGSRWGEFLSDDPREANSEGGVLPAIV
                     GTVIMTLLMSIAVVPFGVLAALYLREYAKAGLAVSAVRIAINNMAGVPSIVFGVFGLG
                     FFCYIIGGSIDQFLFAEKLPNPTYGRGGVLWASLTLALLTLPVVVVSTEEALAAVPRS
                     MREGSYACGASKWQTIRRIVLPRAMPGIMTGFILAMARGAGEVAPLMLVGAVKLAPDL
                     PIDGVAPYLHPDRSFMHLGFHIYDVGFQSQNSEAGKPMVFTTTLLLITIIAFLNIAAV
                     WLRTRLRRRFVGGTF"
     gene            1759..>2204
                     /locus_tag="VEC56_06690"
     CDS             1759..>2204
                     /locus_tag="VEC56_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV13877.1"
                     /translation="MTDPSIQPQPRPDDPNASADSLTRLQAIARGESVPATIHDAVAR
                     EEAVLEVRGFNFWYGSKQALYSIGMGMPKGKITAIIGPSGCGKSTLLRSINRLNDLLD
                     DVRIDGDILLNGDSIYGRGVDVIELRKRMGMVFQKSNPFPMSIYENV"
BASE COUNT          359 a          738 c          759 g          348 t
ORIGIN      
        1 agcacgccgg tcgcatcgcc gaaccacagc ccgccgcgca aggcgccgcg cgcgcgcggg
       61 gggacgtcgc tcctcgccca gggcgaaaac atgctctggc tcacgggagg ggcgctggcg
      121 gtgtgctcgg tcatgatcgt cggcttcctg gtgctggtgc tggcgcaagg aaccgcgacc
      181 ttctggccca agccgctggt cgaagtgacg ctcgacgacg ggcgcgtgct cctcggcgag
      241 gtgacgcgca cggagcgatt cacgcccacg ccggagacgg tcgcgggctg gtccgagcaa
      301 gcgcgcgcgc gcttccagaa cgacgcggcg gaacgcggcg gccgggccac gcgccggctg
      361 gttcgcaccg ggaacttcga gctcaccggg acccacttcg agtggatcga cgacgtcgcc
      421 gtggtgcgcg agtcgcgccc cgactgggcg acgctgctcg agcggcgcgc gtgggggcgc
      481 ttctacggcg ttcccaagga gtttcgggtc gacgggaacg cggttgcgag caccccgagc
      541 gcggcctggc gcgagttccg tcgccaccac gacgcggtcg cgacccgctt cgagcgccgc
      601 taccaactgc agaagcgcga cttcggtgag ctgcacgcgc gcgcggaagg cgcccgcctc
      661 gaggccaagg aggtcgaggc gaaatacggg gaccaatcac cccagcacga gaccgcgctg
      721 cgcatgctgg aaggcgagat gcgggccatc gaatcgcagc gccgcacgct cgaagaggaa
      781 gtgcgcctgc tcgacgaaga gaacgcgcgc tacgaattgg tgctgcagac gacttccgga
      841 gaagatgcgg cgattccgct cgccgacgtg gtgcgcgcat ttccggccaa ccaactctcg
      901 gccgcctcca agctgggcgt ctacgggtcg cgctggggcg aattcctctc cgacgatccg
      961 cgcgaggcca acagcgaagg cggagtgctg ccggccatcg tgggaaccgt gatcatgacg
     1021 ttgctgatgt cgatcgccgt ggtgcccttc ggagtcctgg cggcgctgta cctgcgcgag
     1081 tacgccaagg cggggctggc cgtgagcgcg gtgcgcatcg cgatcaacaa catggccggc
     1141 gtgccgagca tcgtgttcgg cgtgttcggc ctgggcttct tctgctacat catcggcggt
     1201 tcgatcgacc agttcctctt cgcggagaaa ctcccgaacc cgacctacgg gcgcggcggc
     1261 gtactgtggg cgtcgttgac gctcgcactc ctcacgctgc cggtggtggt ggtctcgacc
     1321 gaggaagcgc tggcggcggt tccgcgctcg atgcgcgaag ggtcctacgc gtgcggggcg
     1381 agcaagtggc agaccatccg gcgcatcgtg ctcccgcgcg cgatgcccgg catcatgacg
     1441 ggattcatcc tggccatggc gcgcggcgcc ggcgaagtcg cgccgctgat gctggtgggg
     1501 gcggtcaagc tcgcgcccga cctccccatc gacggggtcg cgccgtacct gcaccccgac
     1561 cgcagcttca tgcacctcgg cttccacatc tacgacgtgg gattccagag ccagaacagc
     1621 gaggccggaa agccgatggt gttcacgacc acgctgctct tgatcacgat cattgcgttt
     1681 ctcaacattg cggcggtgtg gctgcgcacg cggttgcggc ggcgcttcgt cggtggtact
     1741 ttctaaccaa gtaatcgcat gaccgaccct tcgattcagc cccagccccg tcccgacgac
     1801 cccaacgcgt ccgcggactc gctcacgcgc ctgcaggcga tcgcgcgcgg cgagagcgtt
     1861 cccgccacca tccacgacgc cgtggcgcgc gaagaggcgg tgctcgaagt gcgcggcttc
     1921 aacttttggt acggcagcaa gcaggccctg tactcgatcg gcatggggat gccgaagggc
     1981 aagatcacgg ccatcatcgg gccgtcgggg tgcggcaagt cgacgctctt gcgctcgatc
     2041 aaccgcttga acgacctcct ggacgacgtg cgcatcgacg gcgacatcct cttgaacggg
     2101 gactccatct acggccgcgg cgtcgacgtg atcgagctgc gcaaacgcat gggcatggtg
     2161 tttcagaagt cgaacccatt ccccatgagc atctacgaga acgt
//
LOCUS       DASPGK010000246         5132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_414900, whole genome shotgun sequence.
ACCESSION   DASPGK010000246 DASPGK010000000
VERSION     DASPGK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414900"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VEC56_06695"
     CDS             <1..825
                     /locus_tag="VEC56_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HXV13878.1"
                     /translation="EGHDSTPKGVDRLYDFHGRDATVIWDVTDPSNPDTLGVIDDPSI
                     VYHHSGDVTTDQRYLYLCDELATGSIPDITIWDILDPAQPVRVGQIADAATVHNIYIV
                     DDLAYVAYYSSGFRVYDVSNPTAPVLAGQYDTSKRSGESFIGAIGAYVYLPSGNILVC
                     DIENGLFAFTVTPVVASDGPAEADFLLEQNFPNPFNPSTRIPFELTRGGRVALSIYDV
                     AGRRVRAVLDRVMPAGLHETEWDGRDDGGRAVSSGVYFYRMQAGSRTETRRMVLLK"
     gene            882..1652
                     /locus_tag="VEC56_06700"
     CDS             882..1652
                     /locus_tag="VEC56_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV13879.1"
                     /translation="MIEVRGVTKTFKLSRHQKREMGSGFQGSSVDAVSNVSFVCKPGR
                     VFGLLGPNGAGKTTALRLIATTLRPTAGHIVVQGFDTVREPERVRAALGVLTGSTQLY
                     DRLTPNELVRYYADLHDVPRGDFDRRRAEVFAALDMNAYADRRIAKLSTGMRQKVSIA
                     RTMIHDPEVLVLDEATAGLDVLASRGIVELIRRARDRGKTVLFSTHRMGEVDQLCDDL
                     ALIHRGRVLYDGTYDAFKREMQAASFEDEFIRRIEAAG"
     gene            1659..2852
                     /locus_tag="VEC56_06705"
     CDS             1659..2852
                     /locus_tag="VEC56_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV13880.1"
                     /translation="MKTISTVFRKELLDSLRDRRTIVFMIVIPLLLFPVLFRIMMSVE
                     KSQADKERAKKLRVACIDRGNAADFVATLRERGDVVLVGPVAVDSVAALIKADSLDGA
                     FVFAQDFDEDVAEMRPGRVDFYYKSTDDRAIVQDRLRKPLEEYENTLLAQRFARLELD
                     STIVDAVDLRSQNVASIEERVGKSIGGMLPYIFIIFCFMGAMYPAIDLGAGEKERGTM
                     ETLLTAPVNRFHILLGKFGVVVLSGLVSAGVSIFGLFIALKQSNEIPSQFMSIIMKML
                     GWDTIVLVLSLLLPLTIFFAGILLSVSLTARSFKEAQSMISPLNIAVIVPAAIGLVPG
                     IKMSYATAMIPILNVSLATKEIIAGTMQTPHLVVVYTSLIALALLSLYGSVWWFKRES
                     TIFRS"
     gene            2893..4023
                     /gene="mutY"
                     /locus_tag="VEC56_06710"
     CDS             2893..4023
                     /gene="mutY"
                     /locus_tag="VEC56_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXV13881.1"
                     /translation="MTRALLDWYAEAQRDLPWRHARDPYRIWVSEVMLQQTQVRTVIP
                     YYERFLARFPDLASLAGAPLDEVLKLWEGLGYYARARNLHAAARAVVAEAGGTVPSQP
                     DRFAELPGVGVYVCAAVMSIAFGRPLAAVDGNVKRVLARTFGVEDEVDRPTGASAIQA
                     IADRLLDRDDPGGFNQAMMELGALVCRPRDPDCPACPWCDACVARAEDAPLDYPRKSA
                     RRSVPTARIAVGVVARRGRVLITRRAESAMLGGLWEFPGGKIRDRESAEAACRREIRE
                     ETGLDVDVLAKVTTVRHAYSHLKVEIDVFRCVHRKGRVRLRGPVDHRWILLEETGSYA
                     FPKANHKFIPVLKGEPAPGKRRRTAESAKRKRPSRRPARPGA"
     gene            4099..4692
                     /locus_tag="VEC56_06715"
     CDS             4099..4692
                     /locus_tag="VEC56_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXV13882.1"
                     /translation="MKIRHIAGAMALAAVVFAPAAQAQDTYTIDPVHSSTSFKVKHMM
                     VSDVKGAFETFSGAITLDPKNVENSSVEVTIESASISTRNEKRDGHLKSADFFDVEKF
                     PTITFKSKKIAKKGESWVAVGDLTIRGVTKEIELPFTLSGPVSAGNASIIGVSASTEI
                     NRQDFGVSWNKTLDAGGVVVSDKVRIELEVEAKKQAS"
     gene            4867..>5132
                     /locus_tag="VEC56_06720"
     CDS             4867..>5132
                     /locus_tag="VEC56_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXV13883.1"
                     /translation="MQEILTGIIAILYASAPYLLLGFALAGFIHVALARFPRVTALLT
                     GTGRRPILWAALMGIPMALCSCSVLPAARALRREGASKGATASFL"
BASE COUNT          837 a         1748 c         1660 g          887 t
ORIGIN      
        1 gaggggcacg attcgacgcc caagggcgtc gaccgcctct acgacttcca cggccgcgac
       61 gcgaccgtga tctgggacgt caccgatccc tccaatcccg acaccttggg cgtgatcgac
      121 gacccctcga tcgtgtatca ccacagcggc gacgtcacca cggaccagcg ttacctctat
      181 ctgtgcgacg agctggcgac cgggtcgatc cccgacatca cgatctggga catcctcgac
      241 cccgcgcagc cggtgcgcgt gggccagatc gccgacgccg ccaccgtgca caacatctat
      301 atcgtcgacg acctcgcgta cgtcgcgtac tactcttccg gcttccgcgt gtacgacgtc
      361 tccaatccca ccgcgccggt gctggcgggc caatacgaca cctcgaagcg ctcgggcgag
      421 tcgttcatcg gcgccatcgg cgcgtacgtc tatttgccca gcggcaacat tctcgtgtgc
      481 gacatcgaga acgggctgtt cgcattcacg gtgacgccgg tggtcgcgtc ggacggtccc
      541 gcggaggccg acttcctcct cgagcagaac ttccccaacc ccttcaatcc gtcgacgcgg
      601 attcccttcg agctgacgcg cggcgggcgc gtggcgctct cgatctacga cgtggcgggg
      661 cggcgcgtac gcgcggtctt ggaccgcgtc atgcccgccg gcctccacga gaccgagtgg
      721 gacggccgcg acgacggcgg gcgcgccgtc tcgtcgggtg tctatttcta ccgcatgcag
      781 gcgggctcgc ggaccgagac ccggcgcatg gtgcttctca agtaggctgg cgccaacccc
      841 cgcttgccgg cccccgaaac ggctgctatc ctgtcccgac catgatcgaa gtccgcggcg
      901 tcaccaagac gttcaagctc tcgcgccatc agaagcgcga gatgggttcg ggtttccagg
      961 gcagctcggt cgacgcggtg tcgaacgtga gcttcgtgtg caagcccggg cgcgtattcg
     1021 gactgttggg ccccaatggc gccggcaaga ccacggcgct gcgcctgatc gccaccacgc
     1081 tgcggccgac cgcgggccat atcgtcgtcc agggcttcga caccgtgcgc gagcccgagc
     1141 gcgtgcgcgc ggcgctggga gtcctgactg ggtccacgca gctctacgac cgtctcaccc
     1201 ccaacgagct ggtgcgctac tacgccgacc tgcacgacgt tccgcggggt gacttcgacc
     1261 gccgccgcgc cgaggtcttc gccgccctcg acatgaacgc gtacgccgac cggcggatcg
     1321 ccaagctctc gaccggcatg cgccagaaag tttccatcgc gcgcaccatg atccacgacc
     1381 ccgaggtgct ggtcttggac gaagccaccg ccgggttgga cgtgctggcc tcgcgcggca
     1441 tcgtcgagtt gatccgccgc gcgcgcgacc gcggcaagac cgtgctcttc tcgacccatc
     1501 gcatgggcga ggtcgaccag ctgtgcgacg acctggctct gatccaccgc gggcgcgtcc
     1561 tctacgacgg cacctacgac gcgttcaagc gcgagatgca ggccgcctcg ttcgaggacg
     1621 agttcatccg ccgcatcgag gcggcggggt agcggcccat gaaaaccatc tccaccgtct
     1681 tcaggaagga actgctcgac tcgttgcgcg accggcgcac gatcgtgttc atgatcgtga
     1741 ttccgttgct cctgttcccg gtcctgttcc gcatcatgat gtcggtcgag aaatcgcagg
     1801 ccgacaaaga gcgcgccaag aaactgcgcg tcgcgtgcat cgaccgcggt aacgccgccg
     1861 acttcgtcgc caccctgcgc gagcgcggcg acgtcgtgct ggtgggtccg gtggcggtcg
     1921 acagcgtggc ggcgttgatc aaggccgaca gcctggacgg cgccttcgtg ttcgcgcaag
     1981 atttcgacga agacgtggcc gagatgcgac ccggccgcgt cgacttctat tacaagtcga
     2041 ccgacgaccg cgccatcgtg caggatcggt tgcgcaaacc gctcgaagag tacgagaaca
     2101 ccctcttggc ccagcgcttc gcgcgcctcg agctcgactc gaccatcgtc gacgcggtcg
     2161 acttgcgcag ccagaacgtg gccagcatcg aagagcgcgt gggcaagtcg atcggcggca
     2221 tgctgcccta catcttcatc atcttctgct tcatgggcgc gatgtacccg gccatcgacc
     2281 tgggcgcggg cgagaaggag cgcggcacca tggagacgct cttgaccgcg ccggtcaacc
     2341 gcttccacat cctgctgggc aagttcggcg tggtggtgct ctcgggtctg gtgtcggcgg
     2401 gggtgtcgat cttcgggttg ttcatcgccc tcaagcaatc caacgagatc ccgtcccagt
     2461 tcatgagcat catcatgaag atgctggggt gggacaccat cgtactggtg ttgagcctgc
     2521 tcttgccgct gacgatcttc ttcgccggaa ttctgttgtc ggtatcgctg acggcgcgct
     2581 ccttcaaaga ggcgcagagc atgatatccc cgctcaacat cgcggtgatc gtcccggccg
     2641 cgatcggcct ggtcccgggc atcaagatga gctacgccac ggccatgatc cccatactca
     2701 acgtatcgct cgcgaccaag gagatcatcg cgggaaccat gcagactccg catctcgtcg
     2761 tggtgtacac gtcgctgatc gcgctcgccc tcctgagtct gtacggctcg gtgtggtggt
     2821 ttaagcgcga gtcgaccatc ttccgttcct gagtttccga tggcgatgcc gcccgcacac
     2881 cgacttcgtg gcatgacgcg ggcactcctc gattggtacg cggaggcgca gcgcgacctg
     2941 ccgtggcggc acgcgcgcga tccgtaccgt atctgggtca gcgaagtcat gctgcagcag
     3001 acccaggtcc gcaccgtgat cccctactac gagcgctttc tcgctcgctt ccccgacctc
     3061 gcgtcgctcg cgggcgcacc cctcgacgag gttctgaagc tgtgggaagg actgggctac
     3121 tacgctcgcg cgcgcaatct ccacgccgcc gcgcgcgcgg tggtcgcgga agctggcggt
     3181 acggtcccca gccagcccga ccggtttgcc gagctcccgg gcgtgggggt ctacgtttgc
     3241 gccgcggtga tgtcgatcgc cttcgggcgt ccactcgcgg ccgtggacgg caacgtgaag
     3301 cgcgtgctcg cgcggacctt cggcgtcgaa gacgaagtgg accggccaac cggggcgagc
     3361 gcgatccaag cgatcgccga ccggctcctc gaccgtgacg acccgggagg cttcaaccaa
     3421 gcgatgatgg aactgggcgc gctggtgtgc cgcccgcgcg accccgattg ccccgcgtgc
     3481 ccgtggtgcg acgcgtgcgt cgcgcgcgcc gaagacgctc ccctcgacta cccgcgcaag
     3541 tcggcgcgcc ggtcggtgcc gacggcgcgc atcgcggtgg gcgtggtcgc gcgccgtggg
     3601 cgcgtgctca tcacgcggcg ggccgagtcg gccatgctgg gcggcctgtg ggagttcccc
     3661 ggcggcaaga ttcgcgatag agaaagcgcg gaggccgcgt gccggcgcga gatacgggag
     3721 gagaccggcc tcgacgtcga cgtcctcgcg aaggtgacca cggtgcgtca cgcctattcg
     3781 cacctcaagg tggagatcga cgtgttccgg tgcgtgcacc gcaagggacg cgtgcgcttg
     3841 cgcggaccgg tcgaccaccg ttggatcctg ctcgaggaaa ccgggagcta cgccttcccc
     3901 aaggcgaacc ataagttcat accggtgttg aaaggcgagc ccgcgcccgg aaaacggcgc
     3961 cggaccgcgg agtcggccaa acggaagcgc ccctcgcgcc gaccggctcg cccgggcgct
     4021 tgacgcgggc gcgcctcggg attaacttcc cgtgtactcc atttcccata tatccagaaa
     4081 caccgacgga ggttggttat gaagattcga cacatcgccg gcgcgatggc gctggccgca
     4141 gtggtgtttg cgcccgccgc gcaggcgcag gacacctaca ccatcgatcc ggtccactcg
     4201 tcgaccagct tcaaggtcaa gcacatgatg gtgtccgacg tgaagggcgc cttcgagacc
     4261 ttctcgggag ccatcacgct cgaccccaag aacgtcgaga attcgtcggt cgaggtcacc
     4321 atcgagtccg cgagcatctc gacccgcaac gagaagcgcg acgggcactt gaagagcgcg
     4381 gacttcttcg acgtcgagaa gtttccgacc atcaccttca agagcaagaa gatcgcgaag
     4441 aagggtgaga gctgggttgc ggtcggcgac ttgacgattc gcggcgtcac caaggagatc
     4501 gaactcccgt tcacgctcag cggtccggtg agtgccggca acgcgtccat catcggcgtg
     4561 tcggcctcga ccgagatcaa ccgccaggac ttcggggtgt cgtggaacaa gaccctcgac
     4621 gcgggtggcg tggtcgtcag cgacaaggtt cgcatcgagc tcgaagtgga ggccaagaag
     4681 caggcgtcgt aactccggct gaacatcggg cgaatgtccg cctgcgcgcc gcgtcccgca
     4741 ccccggggcg cggcgctccg gttttagacc aaggctaaac cgaatcgcgg gcgccatttg
     4801 ctcccgggcg ccggcggccc cggagtggta tattgcatgc atcgctccgc gcggagcgac
     4861 cctcgtatgc aggagattct caccggtatc atcgcgattc tatacgcgtc cgcgccctac
     4921 ttgctgctgg ggttcgcgct cgcgggcttc attcacgtcg ccctcgcgcg ttttccgcgc
     4981 gtcacggccc tgctgaccgg tacgggccgg cgtccgatcc tgtgggcggc gctcatgggg
     5041 atccccatgg cgctgtgttc gtgcagcgtg ctccccgccg cgcgcgcgct gcgacgcgag
     5101 ggcgcaagca agggtgccac ggcttctttc ct
//
LOCUS       DASPGK010000247         4987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_299104, whole genome shotgun sequence.
ACCESSION   DASPGK010000247 DASPGK010000000
VERSION     DASPGK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299104"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..971)
                     /gene="trxB"
                     /locus_tag="VEC56_06725"
     CDS             complement(42..971)
                     /gene="trxB"
                     /locus_tag="VEC56_06725"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXV13884.1"
                     /translation="MNTRDVIILGSGPAGLTAAIYAARADLKPLVIEGAQPGGQLTIT
                     TDVENYPGFEQPIMGPELMEIMKKQAARFGTEFDFATVERVELDGPVKTLHADGKTYR
                     TRALIVATGASARWLGIPSEEKLKGHGVSACATCDGFFFRNQVIVVVGGGDTALEEAT
                     FLTKFGSVVYLVHRRDQLRASQAMQHRAFNNPKIKFIWNTVVTQVEGEGKVSGVRLRD
                     VVTGKEHTLDCSGLFVAIGHTPNTEIFKGLLAMDESGYLLVEPGSAKTNVPGVFAAGD
                     VADHVYRQAVTAAGMGCMAALDAERYLGEMGGH"
     gene            1123..2385
                     /locus_tag="VEC56_06730"
     CDS             1123..2385
                     /locus_tag="VEC56_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13885.1"
                     /translation="MRSKRSILVAALALTLLAWSCGDDPATVQPDPEPPPPGEDVTPP
                     EAIADLSLSYDPSTFDVRIAWTAPRDDGDHDRVARYQIRYSFSFPMSWWTAVQVADPP
                     TPQAAGSPQSYTIPAPGRGRYVYAAIRSLDAADNFSPVSSVAEVRIPGYGFEAACADA
                     RTGVPVEGLDVLVTERFTHQVVTGADGVVALADLTGGSLTIRIDTGAAPATYHSLQEA
                     FVIDTDHAVDYPMIEFETVSAPVFENLLAMLWEACGRSPVLLKWRSLPVEWYAPAFVN
                     ANGLDYYDAAARAAARWNEATGLPLFVAVGAPAAVGVHMEFLPPASMGGQNGITSYTN
                     DGEGYPLVDRIRIVDSFADGDRLYSIMLHELGHTIRLRHLNDPSYIMFASQPLPDDIS
                     NDEVRLVQLLTALPNAVKLEVYDRSSPP"
     gene            complement(2602..>4987)
                     /locus_tag="VEC56_06735"
     CDS             complement(2602..>4987)
                     /locus_tag="VEC56_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV13886.1"
                     /translation="VAWMLPALVVASCAKKEAPPTEQARELAADEKLRFEQLTIRQIM
                     QGPGIAGTAPSSPRFSADGRSVFFEWNDPARLDSMNAGDPENAYQHYLDLAHEAATYR
                     LDVATRRVEKLAKAAADSTAPSEFAWDRARRRRAEIRGGDVFLVDLEPARTRRITQTL
                     AAEQSVQISPDGATVYFQRDNNLYSIPWGGAPVRQLTNLQFAADPDDAAKPSAQRQFL
                     IDQQKELFVEFKERGDENEKARPKPVYLGAGMELQRMVVSASGRYAALGLNKPTTGGR
                     APVIPLVVTESGYVETEEVRTKVGDEQDEASVVFIDLEGDSLVEIDAGEKISPSVLSW
                     SPTADVVLLRGISEDWHDRLFMTASPGERGPEGSVSARVVDRFHDAAWVDGPMFDESG
                     AWMPDGKSIWFVSEPGGWGHLYVASLSGRRTQLTSGEFEVHSAWLDEDHARWFVVTNE
                     GRPGSRRLWSMDLDGSHRTLVTAEPGTYDVVFAPDFATAAVLRSRATAPAELYLFDAS
                     SGSLDGPVTTSTTAVFRSFPWLEPETVWFTASDGVSVPAHLFKPERYGGTPNGAGVIF
                     IHGAGYLQNVVDGWSYYYREFMFHHFLASRGYTVLNVDYRGSAGYGRDCRTAIYKHMG
                     GRDLDDIVDGARYLVREHGVGAKQIGTYGGSYGGFLTLMAMFKYPNEIAAGSALRSVT
                     DWAHYNHGYTVRILGTPAADPEAYRRSSPIYFAEGLEGDLLMLHGLRDDNVLAEDILR
                     LSQRLIELGKEDWELALHPVERHGYRRAASWTDQITRTFKLFERTLPAGAGGGG"
BASE COUNT          790 a         1700 c         1663 g          834 t
ORIGIN      
        1 gtttcatgcg aggaaagtgt acgcgttgga agcggccggg ctcaatgccc gcccatctcg
       61 cccaggtagc gctccgcgtc gagagccgcc atacacccca tgccggcggc agtgaccgcc
      121 tggcggtata cgtggtcggc cacgtcgccg gccgcgaaca cgcccggcac gttggtcttg
      181 gcgctgcccg gctcgaccag caagtagccg ctctcatcca tggccagcag acccttgaag
      241 atctcggtgt tgggcgtgtg tccgatggcg acgaagagcc cgctgcaatc gagcgtgtgc
      301 tccttgcccg tcaccacgtc gcgcaggcgc acgccgctca cctttccctc gccctcgact
      361 tgcgtcacca ccgtgttcca gatgaacttg atcttggggt tgttgaacgc gcggtgctgc
      421 atcgcctgcg aggcgcgcaa ctggtcgcgc cgatggacca gatacacgac cgagccgaac
      481 ttggtgagga acgtcgcttc ttcgagcgcg gtgtcgccgc cgccgaccac gacgatcacc
      541 tggttgcgaa agaagaaacc gtcgcaggtg gcgcacgcgg agacaccgtg gcccttgagc
      601 ttctcttcgg agggaatccc gagccagcgc gcggaagcac ccgtcgccac gatcagggcg
      661 cgcgtgcggt acgtcttccc gtcggcgtgc agcgtcttga cgggcccgtc caattcgacg
      721 cgctcgacgg tggcgaaatc gaactcggtg ccgaagcgcg cggcctgctt cttcatgatc
      781 tccatgagct cgggacccat gatgggctgc tcgaagccgg gatagttctc cacgtcggtg
      841 gtgatggtga gttggccgcc cggctgcgcg ccctcgatca cgagtggctt cagatcggcg
      901 cgggccgcgt aaatcgcggc agtcaggccg gccgggccgg agcccaggat gatgacgtcg
      961 cgtgtgttca taggatgact cgggagcggc cctcggtgga aaggcccgtt gaatataccc
     1021 ggctgggcat cgaagtcaag cgaacgcgct tgcgcgctcc cggcgcgtct cctaccatga
     1081 tagcgagccc gtcgagtcga cccgccccac ggcccatcgc ccatgcgctc gaagcgatcg
     1141 atactcgtgg ctgcgctcgc gctcacgctg ctcgcgtggt cgtgcggcga cgaccccgca
     1201 accgtccaac ccgaccccga gccgccgccc cccggggaag acgtgacgcc ccccgaggcc
     1261 atcgccgacc tctcgctctc gtacgaccct tccaccttcg acgtgcgcat cgcgtggacc
     1321 gcgccccgcg acgacggcga ccacgaccgc gtcgcgcgct accagattcg ctactcgttc
     1381 tcgttcccga tgtcctggtg gacggcggtg caggtcgccg acccacccac cccgcaggcg
     1441 gcgggatcac cgcagtcgta cacgattccc gcgcccggac gcgggcgcta cgtgtacgcg
     1501 gccatccgtt cgctcgacgc tgcggacaat ttctcgccgg tcagttcggt tgcggaagtt
     1561 cgcatccccg gctacgggtt cgaagccgcg tgcgcggacg cacgcaccgg ggttcccgtc
     1621 gaggggctcg acgtcctcgt caccgaacga ttcacacacc aggtcgtgac gggcgcggac
     1681 ggcgtcgtgg cgctggccga cttgaccggg ggctcgctca cgattcgtat cgacacgggt
     1741 gcggcgcccg cgacgtacca ttcgctccaa gaggccttcg tgatcgacac ggatcacgcg
     1801 gtcgattacc cgatgatcga gttcgaaacc gtgtcggcgc cggttttcga gaacctgctc
     1861 gcgatgttgt gggaagcctg cggccgcagt ccggtcctcc tgaagtggcg gagcttgccg
     1921 gtggagtggt acgcgcccgc gttcgtcaac gcgaacggcc tcgactatta cgacgcggcc
     1981 gcgcgcgccg cggcgcgctg gaacgaggcg accgggttgc cgctcttcgt cgcggtgggt
     2041 gcgccggcgg cggttggggt ccacatggag ttcctccccc cggcgtcgat ggggggccaa
     2101 aacggcatca cgagctacac caacgatggg gagggctatc cgctggtcga tcgcattcgc
     2161 atcgtcgatt ccttcgccga cggggaccgt ctctacagca tcatgctgca cgagctcggg
     2221 cacaccatcc ggctgcggca cctcaacgat ccgtcgtaca tcatgttcgc cagccagccg
     2281 ctgccggacg acatctccaa cgatgaagtc cggctggtcc agctcctgac cgcgctcccc
     2341 aacgcggtca agctcgaggt ctacgaccgt tcatcgccgc cctgagtgaa cagaaaaaag
     2401 ccttccctcc gccacgccgg ttgagacgcc gcggggagaa gcagcatctc gtgtggatcc
     2461 gtcgcaacga gagggaagga cacaggaagt ctcggatcga atcttttgcg ggttcgcacc
     2521 gcatgatgcg gttcggcgat acgaacagac ccccatttta ccacgtgcgc cgggcagttc
     2581 aaccccggat tttcgatccc cttagccccc gcccccggcg cccgccggca gtgtgcgctc
     2641 gaacagtttg aacgttctgg tgatctggtc ggtccacgag gcggcgcggc ggtagccgtg
     2701 gcgctcgacc gggtgcagcg ccaattccca gtcttcctta cccaattcga tcaggcgctg
     2761 gctcaatcgc aggatgtcct ccgccagcac gttgtcgtcg cggagcccgt gcagcatgag
     2821 caagtcgccc tccaggccct cggcgaaata gataggcgac gagcgacggt aggcttcggg
     2881 gtcggccgcg ggggttccga gaatgcgcac cgtatagccg tgattgtagt gggcccagtc
     2941 ggtcaccgaa cgcagcgcgg agccggccgc gatttcattg gggtacttga acatcgccat
     3001 caaggtcagg aagccgccgt aggaaccgcc gtaggtgccg atctgcttcg cgcccacccc
     3061 gtgttcgcgc acgaggtagc gcgcgccgtc gacgatgtcg tcgaggtcgc gcccgcccat
     3121 gtgcttgtag atcgccgtgc ggcagtcgcg cccataaccg gcggagccgc gatagtcgac
     3181 gttgagcacc gtgtacccgc gtgacgcgag gaagtggtgg aacatgaact cgcggtagta
     3241 gtagctccac ccgtcgacca cgttctgcag gtaccccgcg ccgtggatga agatcacacc
     3301 cgcgccgttg ggtgtgccgc cgtagcgctc cggcttgaag aggtgggccg gcacggaaac
     3361 tccgtcggag gccgtgaacc ataccgtctc cggctcgagc cacgggaagc tccgaaacac
     3421 ggccgtggtc gaggtcgtga cgggaccgtc cagagagccc gacgacgcgt cgaagaggta
     3481 gagctcggcg ggcgcggtcg cgcgcgagcg cagcacggcc gcggtcgcga agtccggggc
     3541 gaacacgacg tcgtaggtcc ccggctccgc cgtcaccagg gttcgatgcg agccgtcgag
     3601 gtccatcgac cacagccggc gcgagcccgg gcgcccttcg ttggtcacga cgaaccagcg
     3661 cgcgtggtct tcgtcgagcc acgcgctgtg cacttcgaac tcgccgctcg tcaactgcgt
     3721 gcggcgaccc gacagcgacg cgacgtacag gtgaccccag ccgccgggct cggacacgaa
     3781 ccagatcgac ttgccgtcgg gcatccacgc gcccgattcg tcgaacatgg gaccgtcgac
     3841 ccaggccgcg tcgtggaagc ggtcgacgac gcgcgccgac acgctgccct cggggccgcg
     3901 ctcgccgggc gaggcggtca tgaagaggcg gtcgtgccag tcctcgctga tgccccgcag
     3961 gagcaccacg tccgcggtcg gcgaccacga gagcacgctc ggcgaaatct tctcgccggc
     4021 gtcgatctcg accagcgagt cgccctcgag gtcgatgaat acgacgctgg cttcgtcttg
     4081 ctcgtcgccc accttggtgc gcacctcttc ggtctcgacg tacccgctct cggtgacgac
     4141 gagcgggatc acgggggcgc gcccgcccgt cgtgggttta ttgagaccga gggcggcgta
     4201 gcggccgctg gccgagacca ccatgcgctg cagctccatc cccgcgccga ggtagaccgg
     4261 cttggggcgg gctttttcgt tctcgtcgcc tcgctccttg aactcgacga acaactcctt
     4321 ctgttgatcg atcaggaatt gccgctgcgc gctgggcttg gccgcgtcgt cggggtcggc
     4381 ggcgaactgg aggttggtca gctggcgcac gggcgcgccg ccccacggaa tcgagtacag
     4441 attgttgtcg cgctggaagt agacggtggc gccgtcgggt gatatctgaa ccgactgctc
     4501 ggccgccaga gtttgcgtga tgcgccgcgt gcgcgcgggc tcgaggtcga ccaggaacac
     4561 atcgccgccg cgaatctccg cgcggcggcg ccgagcgcga tcccacgcga attcggaggg
     4621 cgcggtcgaa tcggccgccg ccttggcgag cttctccacg cgccgggtcg cgacgtcgag
     4681 gcgatacgtg gccgcctcgt gcgcgagatc gaggtagtgc tggtacgcgt tctcggggtc
     4741 gcccgcgttc atcgaatcga gccgcgcggg gtcgttccac tcgaagaaca cgctgcggcc
     4801 gtccgcggaa aagcgcgggc tggacggcgc ggtgccggcg atgcccggcc cctgcatgat
     4861 ctgtctgatg gtgagctgct cgaagcggag cttttcgtcc gcggcgagct cgcgcgcctg
     4921 ctccgtgggc ggggcctcct tcttcgcgca cgacgcgacg acgagagccg gaagcatcca
     4981 cgcgacg
//
LOCUS       DASPGK010000248         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_766222, whole genome shotgun sequence.
ACCESSION   DASPGK010000248 DASPGK010000000
VERSION     DASPGK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766222"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VEC56_06740"
     CDS             complement(<1..339)
                     /locus_tag="VEC56_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13887.1"
                     /translation="MNRIVFIAAIAIAVAGPAGAADYFQQFHHTTIRVKLDDERKWIS
                     GTQSIQYVNNSPDTLREFYLHLYPNAFRNKDVPYQKDESRKYNARLLDVPGDHKGWLD
                     LADLKVDGTAV"
     gene            complement(492..1829)
                     /locus_tag="VEC56_06745"
     CDS             complement(492..1829)
                     /locus_tag="VEC56_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV13888.1"
                     /translation="MASRVLIIDDDESLRKVMGYMLEEAGYDVDRAASAEEGLRVIGE
                     RRPDLVLSDIKMPKKDGIELLADIKRVDPTIPVVILTAFASVETAVEAMKRGASDYLT
                     KPIARDDLTLTVAKTLKLHRLEAENEELRTTIRDRFRFESIIGLSPAMTALFDVLKKI
                     APTDATVLVTGESGTGKELIAKAIHYNSPRRAQRLVAVNCAAIPSELLESELFGHVRG
                     AFTGAIRNKIGKFEQAHGGTLFLDEIGSMPVQLQAKLLRALQEREIERVGDERTIEVD
                     VRVIAATNRSLPALVASGEFREDLFYRLNVVPVHVPPLRERRGDVPLLVKHFVDRFAG
                     GVRIVVTPGAMEALERHRWPGNVRELENFCERAVLMRSRDIIDADAVASQLSALTRES
                     PGGPSLRDIERAAVVEALRASGWNRSRAARRLGVPRHILLYRIKKFGIVEDAS"
     gene            complement(1822..2889)
                     /locus_tag="VEC56_06750"
     CDS             complement(1822..2889)
                     /locus_tag="VEC56_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV13889.1"
                     /translation="MTPLATKRWRIGIVAATTALTVSFHYGILFPSAHGTVIHAIHGR
                     LCYVPIILAAVWFGVRGGLLTALAITALTLPYPKLRGITDHHALVSEYTEMVFYIAIG
                     LMTGVLIERQWRARERSAALERELARRERLSSLGQMAAGLAHEIKNPLGSIQGAAEIL
                     GDDAPAGSKERELFDVLRKESRRLSTVVDDFLGFARPRPPQLAPLDMARAIERAAAQI
                     ALDASARGIEIVRDVAPDLPRVFADAEQVHQVLLNLLFNAVAASREGDRVVIEARGRD
                     GERAVTLAVRDRGAGIAPENLPRVFDPFFTTKENGTGLGLSISHTIVSDHGGTIDVES
                     TPGHGTAVTIVLPARGSDARG"
     gene            3086..3643
                     /locus_tag="VEC56_06755"
     CDS             3086..3643
                     /locus_tag="VEC56_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV13890.1"
                     /translation="MTRARRGDLDAREELFQRFLPVLRRWAHRRLPQNARDLNETSDL
                     VQITLLRALNRIDRFDPRGEGAFLGYLRQILLNVVREEIRRSSRRGPHDSLHETLASP
                     YASMVEQVIGYEQMELYQRGLESLTEEQQQAVLLRVEFGYTYPQIADALGKPSPDAAR
                     MTVVRALADLARFIDRDHGTRSPIR"
     gene            3717..>4681
                     /locus_tag="VEC56_06760"
     CDS             3717..>4681
                     /locus_tag="VEC56_06760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV13891.1"
                     /translation="MTRLFGAGSDAKPPRSESLFLWGPLEVKQPLGAGSFGEVFAAWD
                     PTLARDVALKLRSPEVGALRWLDEARNLARVRHPHVLTVHGADVLDGRAGIWTELITG
                     RTLEQELEISGPFPATETLRIGRDIASALVAVHAAGLVHGDIKASNIMLEDGESPRRA
                     VLVDFGSADQLLGQDDIPPYLVGTPLTMAPEVLDGKPATSASDVYGLGATLFRLLTGR
                     YPVETASLDDLKRAHASGEPRSARSVAPNVSPRLSRVLERALEPDPARRWPDARAFKR
                     ALDDVADPTRRIRARAAAIGASAAALAAIVVLGILIARPGPGPISR"
BASE COUNT          750 a         1577 c         1588 g          766 t
ORIGIN      
        1 caccgcggtc ccatcgactt tgaggtccgc gaggtcgagc cagcccttat gatcgccggg
       61 gacgtcgagc aggcgggcgt tgtacttgcg gctctcgtcc ttctggtagg ggacgtcctt
      121 gttgcggaac gcgttcggat acagatggag gtagaactcg cgtaacgtgt cgggcgagtt
      181 gttgacgtac tggatcgact gggtgcccga gatccatttg cgctcgtcgt cgagcttgac
      241 gcgaatcgtg gtgtggtgga actgctgaaa ataatccgca gcgcccgcag gccctgcgac
      301 tgcgatggcg atggctgcga tgaagacgat tcgattcaat tagactcctc gaacgctgcc
      361 cggcgggtcc tgtcactcgt caccccgcgg cctgacgccg cgggggcccc acgagcgcca
      421 cccgccgggg gacgctcaag agttcaaaca aagcgcgtag gctatcacga aacgcgcgcg
      481 gcatccaccg actacgatgc gtcctccacg attccgaatt tcttaatacg gtagagcagg
      541 atgtggcgcg ggacgcccag gcgccgggcc gcgcgcgaac ggttccatcc gctggcgcgc
      601 agcgcttcga ccaccgccgc gcgttcgatg tcgcgcagcg aaggcccgcc cggggactcg
      661 cgcgtgagcg ccgagagctg cgaggccacc gcgtccgcat ctatgatgtc gcgcgagcgc
      721 atcaagacgg cgcgctcgca gaagttctcc agctcgcgga cgttccccgg ccagcggtgc
      781 cgctcgagcg cctccatcgc gccgggcgtc acgacgatgc gcaccccgcc cgcgaagcga
      841 tcgacgaagt gcttcacgag cagcggaacg tcgccgcggc gctcgcgcaa gggcggcacg
      901 tgcacgggca ccacgttcaa acgatagaac agatcctcgc gaaactcgcc ggacgccacc
      961 agcgccggca gcgaccgatt ggtggcggcg atcacgcgca cgtcgacctc gatggtgcgc
     1021 tcgtcgccga cccgttcgat ctcgcgttcc tgcagcgcgc gcaagagctt ggcctgcagc
     1081 tgcaccggca tcgatccgat ctcgtccagg aacagcgtcc caccgtgcgc ctgttcgaac
     1141 ttgccgatct tgttgcgaat ggcaccggtg aaggcgccgc gcacgtgtcc gaacagctcg
     1201 ctctcgagca gctcggaggg aatggcggcg cagttgaccg cgaccaagcg ctgcgcgcgg
     1261 cgcggactgt tgtagtggat ggccttcgcg atcagttcct tgccggtgcc gctctcgccg
     1321 gtgacgagca cggtggcgtc ggtgggcgcg atcttcttga ggacgtcgaa gagcgcggtc
     1381 atcgcgggcg agagcccgat gatgctctcg aagcgaaagc ggtcgcggat cgtcgtgcgc
     1441 agttcctcgt tctcggcttc gaggcggtgg agcttgagcg tcttggcgac cgtgagcgtc
     1501 aagtcgtcgc gcgcgatggg cttggtcagg tagtccgagg caccgcgctt catcgcttcg
     1561 accgccgtct cgacggaggc gaaggcggtc aagatcacga ccgggatggt gggatcgacg
     1621 cgcttgatgt cggccaacag ctcgatgccg tctttcttgg gcatcttgat gtcggagagc
     1681 acgaggtcgg ggcggcgctc cccgatcact cgcaggcctt cctcggcgga ggcggcgcgg
     1741 tcgacgtcgt agcccgcttc ctcgagcatg tagcccatca ccttgcgcag gctctcgtcg
     1801 tcgtcgatga tgaggacgcg gctagccacg cgcgtcactt ccgcgagcgg gcagcacgat
     1861 cgttaccgcg gtgccgtgac ccggcgtgct ctcgacgtcg atggtgccgc cgtggtcgct
     1921 cacgatggtg tgcgagatcg aaagccccaa gccggtgccg ttctccttag tggtgaagaa
     1981 cggatcgaac acgcggggta gattctcggg cgcgatgccg gcgccgcgat cgcgcaccgc
     2041 gagagtgacc gcgcgttcgc cgtccctgcc gcgtgcctcg atcacgacgc ggtcaccctc
     2101 gcggctagcc gccaccgcat tgaacaagag attcaagagc acctggtgga cctgttcggc
     2161 gtcggcgaag acgcggggta agtccggcgc gacgtcacgg acgatttcga tgccgcgcgc
     2221 ggacgcgtcc agagcgatct gcgccgccgc gcgctcgatc gcgcgcgcca tatcgagcgg
     2281 tgccagctgg ggcgggcgcg ggcgcgcgaa gccgaggaaa tcgtcgacga cggtactcaa
     2341 gcgccgcgat tccttgcgca gcacgtcgaa gagctcgcgc tccttcgatc cagccggcgc
     2401 gtcgtcgccc aggatctccg ccgcgccctg gatcgaaccc agcggatttt tgatctcgtg
     2461 cgccaagccc gcggccatct gtcccagcga cgacaaccgt tcgcgccgcg cgagctcgcg
     2521 ctcgagggcg gcgctgcgct cgcgcgcgcg ccactggcgc tcgatcagca cgccggtcat
     2581 gagcccgatg gcgatgtaga acaccatctc ggtgtactcg gagacgagcg cgtggtggtc
     2641 ggtgatgccg cgcagcttgg gatagggaag ggtcagcgcg gtgatggcga gggcggtcaa
     2701 caggccgcca cgcaccccga accaaaccgc cgccagaatg atgggcacgt agcagagccg
     2761 gccgtggatg gcgtggatca cggtgccgtg cgcggacggg aacaggatgc cgtagtggaa
     2821 cgacaccgtc agcgcggtgg tcgccgcgac gatgccgatt cgccagcgct tggtcgcgag
     2881 gggagtcatg tggagactat agtacatact ctgtaggatt ttcaacaagc caggatgccg
     2941 agcgccacgc cccggcccgg gcgccagccg tgccaccttg tgataagatg gatcgtccgc
     3001 gacggacgga ggtgccccct tggacgatgt gcttcgcccc cccgatggcg ccgaaaatcc
     3061 tcctccctcc gcgaccgcgg tcctcttgac gcgggcgcgc cgtggggacc tggatgcgcg
     3121 cgaggagttg ttccagcgct tcctcccggt tctgcgccga tgggcgcacc gccgcctccc
     3181 ccagaacgcc cgcgacctca acgagacctc cgacctcgtg cagatcacgc tcctgcgcgc
     3241 gctcaatcgc atcgaccgct tcgacccgcg cggcgagggc gcgttcctgg gttaccttcg
     3301 gcagatcctg ctcaacgtgg tgcgcgagga aatccggcgt tcctcgcgcc gcggaccgca
     3361 cgactcgctg cacgagacgc tcgcgtcgcc gtacgcgtcg atggtcgaac aggtcatcgg
     3421 ctatgaacag atggagcttt atcagcgcgg cctggaatct ttgaccgagg agcagcagca
     3481 agcggttttg ctgcgcgtcg agttcggtta cacctacccg cagatcgccg acgcgctcgg
     3541 caagccgagc ccggacgcgg ctcgcatgac cgtggtgcgc gcgctcgccg atctggctcg
     3601 gttcatcgac agggaccatg gaacacgatc cccgatacga tgacgccgag gcggtcgctg
     3661 accgccgccc cgtttccacg acctcgtcct cggacggcat ccgactcctg gaccgaatga
     3721 cccgactctt cggcgcggga tccgacgcca agccgccgcg aagcgaatcg ctcttcttgt
     3781 ggggaccgct cgaggtcaag cagccgctgg gtgccggatc gttcggggaa gtgttcgcgg
     3841 cgtgggatcc gacgctcgcg cgcgacgtcg cactcaaact gcgcagcccc gaagtcgggg
     3901 cgctgcgctg gctcgacgaa gcgcgcaacc tcgcacgcgt tcgccatccc catgttctca
     3961 ccgtgcacgg cgccgatgtg ctcgacgggc gcgccggtat ctggaccgag ctgatcaccg
     4021 gacgcacgct ggagcaagag ctcgagatct ccggcccgtt tcccgcaacc gaaaccctcc
     4081 gtatcggtcg cgacattgcg tccgcgcttg tcgcagtgca cgcggccgga ctcgtgcacg
     4141 gcgacatcaa ggccagcaac atcatgctgg aggacggcga gtcgccgcga cgcgcggtgc
     4201 tcgtggactt cgggagcgcg gaccagttgc tgggacagga cgacattccg ccgtatctgg
     4261 tcggaacccc gctcaccatg gcgcccgaag tgctcgacgg aaagcccgcg acgagcgcct
     4321 cggacgtgta cggcctgggc gcgacactgt ttcgacttct aaccgggcgc tatccggtgg
     4381 agaccgcgtc gctcgacgac ctcaagcgcg cgcacgcgtc gggggagccg cgttcggcgc
     4441 gatcggtcgc gccgaacgtg tcgccgcgcc tctcgcgcgt gttggagcgc gcgctggagc
     4501 ccgatccggc ccggcgttgg ccggatgcgc gcgcgttcaa gcgcgcactc gacgacgtcg
     4561 ccgatcccac gcgacgcatt cgcgcgcgcg ccgcggccat cggggccagt gccgccgcgt
     4621 tggcggccat cgtggtactg ggaatcctta tcgcgcggcc ggggccggga cccatttcgc
     4681 g
//
LOCUS       DASPGK010000249         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_531831, whole genome shotgun sequence.
ACCESSION   DASPGK010000249 DASPGK010000000
VERSION     DASPGK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531831"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VEC56_06765"
     CDS             <1..825
                     /locus_tag="VEC56_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13892.1"
                     /translation="PRFRFDGSTTKGRQRTHMEGGSHITLGPAGKREIEPTFFFMMNC
                     LLYSRLESGNPSNQNYYDEDVNVYLANLLTSFMNPEFHERTRKYLSPYDSDLFEKVRN
                     SPDVRLKYTIYKTNADFLLISIGIFRNPDASRPGAKDAFMKDNEEVYVGRAKAYYQFA
                     YSYSQSMFRKATAISEVLEKLSRGFEQYVDILSYMRGEYFNILERLSSGDVYHLERAI
                     DRVESDRELRALQDQFLDLYSEYMKDKKPELKERLRELAKKIQRIDESFRFTMDQI"
     gene            complement(806..1687)
                     /locus_tag="VEC56_06770"
     CDS             complement(806..1687)
                     /locus_tag="VEC56_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV13893.1"
                     /translation="MSWHGQIDRTIWEINQAIQKSPDQARYRRELLRAQFLQHFERDK
                     AFHVARDDRTFLFLHDGESPLVLLVHGASGTPAEFRELGNFLYARGISTFCPRLTKTT
                     GRGEMVSWGSWVTQAQIALEAVTACSPNAFVCGLSLGGTVSLILHDRAPLSGVILLAP
                     ALYPRMSLKARFLQISRVVTPTVFYHFAGWNGEVIKAMEYARKSSVPIDKPVLALQAA
                     DDRHLSPRGMKFVRRQARHADADIKLLPEGSHVLTRGSAKAEVFQTIASFVERNAKTV
                     APVAREAVEPPSISDPS"
     gene            complement(1713..3176)
                     /gene="guaB"
                     /locus_tag="VEC56_06775"
     CDS             complement(1713..3176)
                     /gene="guaB"
                     /locus_tag="VEC56_06775"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXV13894.1"
                     /translation="MPEKIIGTGITFDDVLLVPAKSSFLPGDADVSTRLTKKIPLNIP
                     IVSAAMDTVTEAQFAIAIAREGGMGFIHRNMTPEAQAEQVDMVKRSESGVISNPITLG
                     PNARVRDAMTLMKKYGISGVPITDKNGVLVGILTSRDLRFNDDDTRPIKDLMTSKGLV
                     TAPVGIKMEDARRLLHEHRIEKLPVVDSKNRIRGLITVKDIQKRMDFPHAAKDSEGRL
                     RVGAAVGVGKDALARVDALVDAGVDAVAIDTAHGHTAGVIDTLTKARKRHRGLQLIAG
                     NIATAQAARDLIKAGADAIKVGMGPGAICTTRVVAGAGVPQITAIMDCAAVASKAGVP
                     LIADGGIKYSGDIVKAIAAGADVVMIGSLFAGTDETPGEIVLFEGRSFKSYRGMGSIE
                     AMKGGSKDRYGQSGVEEAKLVAEGIEGRVPYKGRIADSIYQLIGGLRAGMGYCGTRTL
                     AELKDRSHFMLVTAAALRESHPHDVIITKEAPNYRLS"
     gene            3352..>4022
                     /locus_tag="VEC56_06780"
     CDS             3352..>4022
                     /locus_tag="VEC56_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13895.1"
                     /translation="MVLPALLAVAPAGAQTLSYVGVYPDESRSTCIHTGHPTAVSMAH
                     VFVTNSLPLTEISFSAPIPECLGGYLGEASPFAITGNSQSGVTIDLGACTTAPVKVLT
                     IYYVSAASNPCCAWLTAAHPSSASGQIEFADCEGVTRHGFTAVWSPTPEWTCCYSFDV
                     LSPYQPFPADGATGVSTEVDLNWLLPDQRCNEFFFFGTYPPDHFQDWTRDLDEYTTGY
                     DPGTL"
BASE COUNT          677 a         1334 c         1255 g          756 t
ORIGIN      
        1 ccccgattcc ggttcgacgg ctccacaacg aagggacggc aacgaaccca catggaaggc
       61 ggaagtcaca tcacgctcgg cccggccggc aagcgcgaga tcgagccgac gttcttcttc
      121 atgatgaatt gcctgctcta cagtcgactg gagtccggca acccctcgaa ccagaattac
      181 tacgacgaag acgtcaacgt ctacctcgcc aatttgctga cgtcgttcat gaatccggag
      241 ttccacgaac gcacgcgcaa gtatctctcg ccctacgaca gcgatctgtt cgagaaggtg
      301 cgcaactcgc ccgacgttcg cctcaagtac accatctaca agaccaacgc cgatttcctc
      361 ctgatatcga tcggcatctt tcgcaatccg gacgcgtcgc ggccgggcgc caaggatgcg
      421 ttcatgaagg ataacgagga agtctacgtg ggacgcgcca aggcctacta ccagttcgcg
      481 tattcgtaca gccagtcgat gttccgcaag gcgaccgcga tcagcgaggt gctggagaag
      541 ctctcgcgcg gcttcgaaca gtacgtggat atcttgagct acatgcgcgg ggagtacttc
      601 aatatcttgg agcgtctctc gtcgggcgac gtgtaccacc tcgagcgcgc gatcgaccgc
      661 gtcgagtccg accgcgagct gcgcgcgctg caggatcagt ttctcgactt gtactcggag
      721 tacatgaagg acaagaagcc ggagctcaag gagcggctcc gcgaactagc gaagaagatt
      781 caacgaatcg atgagtcgtt ccgcttcacg atggatcaga tatagacggc ggctccaccg
      841 cttcgcgcgc gaccggcgcc accgtcttcg cattgcgttc cacgaacgac gcaatcgtct
      901 ggaacacttc cgccttggcc gacccccgcg tcagcacgtg cgatccttct gggagtagct
      961 tgatatccgc gtccgcgtga cgcgcctgcc ggcgcacgaa tttcattccg cgcggcgaga
     1021 ggtggcggtc gtcggccgcc tgcagggcga gcaccggctt gtcgatgggc accgaactct
     1081 tgcgcgcgta ctccatcgct ttgatgacct ccccgttcca gcccgcgaaa tgatagaaca
     1141 cggtcggcgt caccacgcgc gagatctgga ggaagcgtgc cttcaggctc atgcgcgggt
     1201 agagcgcggg cgccagcagg atcacgcccg agagcggcgc gcgatcgtgc aggatcaggg
     1261 ataccgtgcc gcccaggctc aatccgcaca cgaacgcgtt cggcgagcac gccgtcaccg
     1321 cctccagcgc gatctgcgcc tgcgtcaccc acgatcccca cgagaccatc tcgccgcgcc
     1381 ccgtcgtctt ggtcagtcgc gggcagaagg tcgagatgcc gcgggcgtag agaaaattgc
     1441 cgagctcgcg aaactcagcg ggcgtgccgc tggcgccgtg caccagcaac accagcggcg
     1501 attcgccgtc gtggaggaac aggaaggtgc ggtcgtcgcg cgccacgtgg aacgccttgt
     1561 cgcgctcgaa gtgctgcaag aactgcgcgc gcaagagctc gcgccggtag cgcgcctggt
     1621 cgggcgactt ctggatcgcc tggttgatct cccagatggt gcggtcgatt tgtccgtgcc
     1681 agctcatgcg tattcgcgtc ccggttggcg cctcagctca gacggtaatt gggcgcttcc
     1741 ttggtgatga tgacgtcgtg ggggtggctc tcgcgcagcg ccgcggccgt gacgagcatg
     1801 aagtggctgc ggtccttgag ttcggcgagc gtgcgcgtgc cgcagtaacc catgccggcg
     1861 cgcaggccgc cgatcagttg gtagatcgaa tccgcaatgc gacccttgta gggcacgcgt
     1921 ccctcgatgc cttccgcgac cagcttggct tcctcgaccc cggactggcc gtagcgatcc
     1981 ttgctaccgc ccttcatcgc ttcgatcgag cccatgcccc gatacgactt gaagctgcgt
     2041 ccttcgaaca ggacgatctc gcccggggtt tcgtcggtgc ccgcgaagag actcccgatc
     2101 atgacgacgt cggcgccggc cgcgatggcc ttgacgatgt cgccgctgta cttgatgccg
     2161 ccgtccgcga tcaacggcac gcccgccttg gaagccaccg ccgcgcagtc catgatcgcg
     2221 gtgatctggg ggacgcccgc gcccgccacc acgcgcgtcg tgcagatggc ccccgggccc
     2281 atgccgacct tgatcgcgtc ggcgccggcc ttgatcaaat cacgcgcggc ctgcgcggtg
     2341 gcgatgttgc ccgcgatcag ctgcagcccg cggtgacgct tcctggcctt ggtcaacgtg
     2401 tcgatcacgc ccgcggtgtg cccgtgcgcg gtgtcgatgg cgaccgcgtc cacgccggcg
     2461 tcgaccaacg cgtcgacgcg cgccagcgcg tcctttccaa ccccgaccgc ggcgcccacc
     2521 cgcaatcgac cctccgagtc cttggccgcg tgggggaagt ccatgcgttt ctggatgtct
     2581 ttgaccgtga tgagtccacg aatgcgattc ttggaatcaa ccaccggtag cttctcgatg
     2641 cggtgctcgt ggagcaggcg ccgcgcgtct tccatcttga tgccgacggg tgcggtcacg
     2701 agacccttcg acgtcatcag gtccttgata gggcgcgtgt cgtcgtcgtt gaagcggaga
     2761 tcgcgcgacg tgagaatccc gaccagcacg ccgttcttgt cggtgatggg tacgcccgag
     2821 attccgtact tcttcatcag ggtcatcgcg tcgcgcacgc gcgcgttggg cccgagcgtg
     2881 atcgggttcg agatcacacc gctctcgctg cgcttgacca tgtccacctg ctcggcctgg
     2941 gcctcgggcg tcatgttgcg atggatgaag cccatgcctc cttcgcgcgc gatcgcgatg
     3001 gcgaactgcg cctcggtgac cgtgtccatg gccgcggaca cgatcggaat gttgagcggg
     3061 atcttcttgg tgaggcgcgt ggagacgtcg gcgtcgccgg gcagaaatga cgacttggcc
     3121 ggaaccagca gcacgtcgtc gaacgtgata ccggtcccga tgatcttctc gggcatggcg
     3181 gggggtccct ccgggttaat tgggtgaatc tatcacgcgg cccccgggcg cgcaagcgac
     3241 gcggccgggg cgtatcgcgg ggtccgttcg cccggggatc tcccgggtgc tatcggtgct
     3301 gcgcggccgc cccgatctgc tatactccgc gctatgcgaa gcgcagcagg cgtggtgctc
     3361 cctgccctgc tcgcggttgc gccggcgggt gcccaaaccc tatcgtacgt cggcgtctat
     3421 ccggacgagt cccgctcgac gtgcatccac acgggtcatc cgaccgcggt ctcgatggcg
     3481 cacgtcttcg tcaccaattc actcccgctc acggagatca gtttttccgc gcccatcccg
     3541 gagtgtctgg ggggctacct cggcgaggca tcgccgtttg ccatcaccgg caactcacag
     3601 tccggcgtga cgatcgatct gggcgcttgc acgacagcac ccgtcaaggt gctgacgatc
     3661 tattatgtgt cggcggcaag caatccctgt tgtgcgtggc tgacggccgc gcacccgtct
     3721 tccgcgagcg ggcagatcga attcgccgac tgcgagggtg tcacgcgcca cggattcacc
     3781 gcggtgtgga gcccgacgcc ggagtggacc tgttgctaca gcttcgacgt tctctcgccc
     3841 taccaaccct tcccggccga cggtgccacc ggcgtgtcga cggaagtcga tctgaactgg
     3901 ctcttgcccg atcagcgctg caatgaattc ttcttctttg gcacctatcc cccggatcat
     3961 ttccaagact ggacccggga tctcgacgag tacaccactg gctacgaccc gggcacgctg
     4021 ca
//
LOCUS       DASPGK010000250         4503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1586601, whole genome shotgun sequence.
ACCESSION   DASPGK010000250 DASPGK010000000
VERSION     DASPGK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586601"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VEC56_06785"
     CDS             <1..1491
                     /locus_tag="VEC56_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXV13896.1"
                     /translation="MGSFPRIPAARRQQVAIATGPLIFLVLLFGFHPVPEQPAVGRTL
                     AVAAWLAVYWLTEAIPLAATALIPVVLFPLLGVMDATAIASEYVNDLIFLFIGGFLLA
                     IAMQEWGLHRRVALRILAWIGHSPATLLLGVMGATWAISWWASNTATTLMMLPIVLAL
                     AAKLEERSGPEARPLTVALLLSVAYGASIGGMATLIGTAPNIVFLRVHALSFPDAPPL
                     TFLTWMAVAAPISTAVGVLAFLYLRARLLRGCRVDVDREMLRAERRSLGAISFEERTV
                     FAVFLVFVALVVTRADVVVGETTIRGWASRIGVGNRMGDGAVAIAAALALFVVPARTR
                     PGFVLEAPAIAKLPWDIVVLFGGGFALAEAFQVSGASHYLGEQLAGLAGAPPLLVMFA
                     VCAVVCFLSELASNTALAQVVLPVLGSMATVTGMHPLFLMVPATMAASCGFMLPVATP
                     PNAIVFGTRRIRTGEMVRAGFAIDWIGIIVVTLAMYTWGRWVLGID"
     gene            complement(1547..2002)
                     /locus_tag="VEC56_06790"
     CDS             complement(1547..2002)
                     /locus_tag="VEC56_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13897.1"
                     /translation="MKRTLIVFTMLCSLSVPSYAQDISELLRADLRANKTTLITQAMA
                     LDPAQFEVFWPIYRAYEADLVKLNDESLAAIKDYAENYANMTDETAKDLLKRGFKIRE
                     GRTKLLKKYSSKVGKALSPKVAARWVQVEHALQAAIDLQMASELPLMGK"
     gene            complement(2037..2705)
                     /locus_tag="VEC56_06795"
     CDS             complement(2037..2705)
                     /locus_tag="VEC56_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13898.1"
                     /translation="MKRVFIVSALVCAAASAASAQYTTPSSSYGSTPAKKADVVFLGG
                     YAWTLSQDVVLNYTNAGELDIEDNPFWGVALDFNVARQGGGKTGQLRLMYRREDSDVV
                     FRSYVPSNPLVSLDCAVEYWQIGGLGGVKRGNAMPFTSVTLGGTRLVAGDVDEWKFSM
                     IFGLGVKVYTEGKVGFMIQGNWPITFTDTWGGVTVGTGGAGVAVGGTGISQLDVGGGV
                     FVSF"
     gene            complement(2719..3267)
                     /locus_tag="VEC56_06800"
     CDS             complement(2719..3267)
                     /locus_tag="VEC56_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV13899.1"
                     /translation="MRFPTLAATAACLLVAGCSSYDIKYDYDVDSNFAAYRTYAWIPQ
                     KISETGTSASVAMQRNTLLDKRIRSAVDANLAAKGMTVSEEAPDLLVVYHTGMQNKVD
                     VTDWGYSYAGSYWGWAGRDIDVYNYTEGTLIVDLVSSQTKQLAWRGSATGVVDPGRSP
                     EEVQERINDVVSQIFANYPPKK"
     gene            3377..4219
                     /locus_tag="VEC56_06805"
     CDS             3377..4219
                     /locus_tag="VEC56_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV13900.1"
                     /translation="MNTDARTKIGLVLGSGGARGYAHIGVLRAIEKRGLEVTAIAGCS
                     MGGIIGALHAAGYSSQRIHEMWKTLDPLKLIDRSTMGGLIGGRGITRHLEQYLPRTFE
                     ELKIPVAVTSVDVQRGKLVVLRSGDLPRALRATSAVAGVFAPEPHEGRILVDGGILNG
                     LPVDVIRTLTRAPVVAVDTTPPADREIAFTDDRSTWEKVRHPIRGRPLVFEMLMKAIE
                     IPQAPLTSIRLSLNPPEVLIRPALEPDLKLEDYRRLDEAVEAGFVAAEAKLAEHETLL
                     RESQ"
     gene            complement(4255..>4503)
                     /locus_tag="VEC56_06810"
     CDS             complement(4255..>4503)
                     /locus_tag="VEC56_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HXV13901.1"
                     /translation="SGVEYRDRPAGWGLERRKEYVAQRYHKPQDQYDATWDLSGAAED
                     MEALFRVGLTLANSDQTPQWSEKSEFRRARDAMMRGSK"
BASE COUNT          694 a         1493 c         1480 g          836 t
ORIGIN      
        1 atggggtcgt tcccccgcat cccggctgcg cgccggcagc aggtcgcgat cgcgaccggg
       61 ccgctcatat tcctggtcct gctcttcgga ttccaccccg tccccgagca gccggcggtg
      121 ggccgtacgc tcgccgtggc ggcgtggctc gcggtctact ggttgaccga agcgatcccg
      181 ctggcggcca ccgcgctcat tcccgtcgtg ctgttcccgc tcctgggcgt gatggacgcg
      241 acggcgatcg cgtccgagta cgtcaacgat ttgatcttcc tcttcatagg cggcttcctg
      301 ctcgcgattg cgatgcagga gtgggggctg caccgccgcg tggcactgcg catcctggcg
      361 tggatcgggc acagccctgc gacactcttg ctcggcgtca tgggcgcgac ctgggccatc
      421 tcgtggtggg cgtcgaacac cgcgaccacc ttgatgatgc tcccgatcgt gctggcgctc
      481 gccgccaagc tcgaggagcg ttcgggtccc gaagcgcgtc cgctgacggt ggcgctcctg
      541 ctctcggtcg cgtacggcgc atccatcgga gggatggcga cgctcatcgg caccgcgccc
      601 aacatcgtgt tcctgcgcgt gcacgcgctc tcgttccccg atgcgccccc gctcacgttc
      661 ctcacctgga tggcggtggc ggcgcccatc tccaccgccg tgggggtgct cgcgttcctc
      721 tatctgcgcg cgcgcctgct gcgcggttgc cgcgtcgacg tcgaccgcga gatgctgcgc
      781 gccgagcggc gatcgctggg cgcgatttcg ttcgaagagc ggacggtgtt cgccgtgttt
      841 ctcgtgttcg tggcactggt ggtgacgcgt gccgacgtgg tggtgggcga gaccacgatt
      901 cgcggctggg cgtcccgcat cggggtgggg aaccggatgg gcgacggagc ggtcgccatc
      961 gcggccgcgc ttgcgttgtt cgtcgtgccc gcgcgcacgc gcccgggctt cgttctggaa
     1021 gcgcccgcga tcgcgaagct cccctgggac atcgtagttc tcttcggcgg cggcttcgca
     1081 ctcgcggagg ccttccaggt ctcgggcgcg tcgcactacc tgggcgagca gctcgcggga
     1141 ctcgcgggcg cgccgccgct cctcgtcatg ttcgcggtgt gcgcggtggt gtgcttcttg
     1201 agcgagctcg cgtcgaacac cgcactcgcg caggtcgttc tgccggtgct gggatcgatg
     1261 gcgaccgtca ccggcatgca cccgctcttt ctcatggtgc cggcgacgat ggccgcctcc
     1321 tgcggcttca tgctgccggt ggcgacgccc cccaacgcga tcgtgttcgg gacgagacgg
     1381 attcgcaccg gcgagatggt gcgcgcgggc ttcgcgatcg attggatcgg catcatcgtc
     1441 gtaaccctcg cgatgtacac gtggggccgg tgggtgctcg gcatcgactg aaaaaagggc
     1501 gcccgccttc cggcgagcgc ccgtagatcg aatctcccgc gagaggttac tttcccatca
     1561 acggcagctc ggacgccatc tggaggtcga tggcggcctg gagcgcgtgt tcgacctgaa
     1621 cccagcgcgc cgcaactttg ggggacaggg ccttgccgac cttgctggag tacttcttca
     1681 ggagcttggt tcgcccttcg cggatcttga agccgcgctt gagcaggtcc ttcgccgtct
     1741 catcggtcat gttggcgtag ttttcggcgt agtccttgat cgcggccagg ctctcgtcgt
     1801 tgagcttaac cagatcggct tcatacgctc ggtagatggg ccagaagacc tcgaactgcg
     1861 ccgggtcgag ggccatcgcc tgggtgataa gcgtggtctt gttggcgcgc aggtcggccc
     1921 gcaacaactc gctaatgtcc tgcgcgtacg acggcaccga caggctgcag agcatcgtga
     1981 agacgatcag ggtccgtttc atcggtaacg attcctttcc gtgccagtgc gggcggttag
     2041 aacgaaacga agacgccgcc gccgacgtcg agctgcgaga taccggtgcc gccgaccgcc
     2101 acgcccgccc cgcccgtgcc gacggtcacg ccgccccagg tatcggtgaa cgtgatcggc
     2161 cagttgccct ggatcatgaa gccgactttc ccttccgtgt acactttgac acccaggccg
     2221 aagatcatcg agaacttcca ctcgtccacg tcgccggcga cgagtcgcgt gccgccgagt
     2281 gtcacggacg tgaagggcat cgcgttgccg cgcttgacac cgcccagccc gccgatctgc
     2341 cagtattcga ccgcgcagtc cagcgatacc aacgggttcg acggaacgta cgagcgaaat
     2401 acgacatccg agtcctcgcg ccggtacatc agtcgaagct ggccggtctt accgccgccc
     2461 tgacgggcga cgttgaaatc gagcgcgacg ccccaaaacg gattgtcttc gatgtccaac
     2521 tcgcccgcgt tggtgtagtt gagcacgacg tcctgcgaca gcgtccaggc gtagccgccc
     2581 agaaagacga cgtcggcctt cttcgccggc gtggacccgt acgacgacga cggcgtcgtg
     2641 tactgcgccg acgcggccga tgccgccgcg cacacgagcg cgcttacaat gaatactcgc
     2701 ttcataatcg cctcccggct acttcttcgg tggataattg gcaaagatct gcgacaccac
     2761 gtcgttgatc cgctcttgca cttcctccgg cgagcgaccc gggtcgacga cgccggtggc
     2821 actgccgcgc cacgcgagtt gtttggtctg ggaactgacc agatcgacga tcagcgtgcc
     2881 ctcggtatag ttgtagacgt cgatgtcgcg cccggcccag ccccagtaac tgcccgcgta
     2941 gctgtaaccc caatccgtga cgtcgacctt gttttgcata ccggtgtgat acaccacgag
     3001 gaggtcgggg gcttcctcgc tcaccgtcat gcccttggcg gccaggttgg cgtcgacggc
     3061 ggagcggatg cgcttgtcga gaagcgtgtt gcgctgcatc gcgacactgg cgctggtgcc
     3121 cgtttcgctg atcttctgcg ggatccacgc gtacgtgcga tacgcggcaa aattggaatc
     3181 gacgtcgtaa tcgtacttga tgtcgtagct ggaacagccg gcgacaagca ggcatgccgc
     3241 ggtagcggcg agagtcggga aacgcatggc caagctcctt ctcgataatc gaccgtcaca
     3301 ctagccgaag gcggtacgac tgtcaatggc gccgagaatt gacaagttcg acgaagcccg
     3361 tcgataatgg cacgcgatga acacggacgc acgaaccaag atcggactgg tcctgggcag
     3421 cggcggggcg cgcggctacg cccacatcgg cgtgctgcgc gcgatcgaga agcgcggcct
     3481 cgaagtcacc gccatcgccg gctgctcgat ggggggcatc atcggcgccc tccacgccgc
     3541 cggctactcg tcgcagcgca tccacgagat gtggaagacg ctcgacccgc tgaaactgat
     3601 cgatcgcagc acgatgggag gattgatcgg ggggcgcggc atcacgcgcc acctcgagca
     3661 gtacctcccg cgcacgttcg aagaattgaa gatcccggtg gcggtgacgt cggtcgacgt
     3721 gcagcgcggg aagctggtcg tgctgcgcag cggcgatcta ccgcgcgcgc tgcgcgcgac
     3781 cagtgccgtg gccggggtgt tcgcgccgga accgcacgag gggcgtatcc tggtcgacgg
     3841 cggtattctg aacggactgc cggtggacgt gattcgaacg ctgacccgcg cgccggtggt
     3901 cgcggtcgac accacgccgc ccgccgaccg cgagatcgcc tttaccgacg accgctcgac
     3961 ctgggagaag gttcgtcatc ccatccgcgg gcgcccgctg gtattcgaaa tgctcatgaa
     4021 ggcgatcgag atcccgcagg cgcccctgac ctcgattcgc ctgtcgctca acccgcccga
     4081 ggtactgatc cgtcccgcgc tcgagccgga cctgaagctc gaggactacc gacgtctcga
     4141 cgaagcagtc gaagcgggat tcgtggccgc ggaagcgaag ctcgcggagc acgaaacgtt
     4201 gttgcgcgag agtcagtagc cgtctttcct tgccgatgcg aacgcgtcgg tggcctactt
     4261 cgacccccgc atcattgcgt cgcgcgcacg gcggaattcg ctcttctcgc tccactgcgg
     4321 cgtttggtcg ctgttcgcga gcgtgagccc gacacggaac agagcctcca tgtcctcggc
     4381 cgcgccggag aggtcccagg tcgcgtcgta ctggtcttgc ggcttgtggt agcgctgggc
     4441 gacgtactcc ttgcggcgct cgaggcccca gcccgccggg cggtcgcggt actccacacc
     4501 act
//
LOCUS       DASPGK010000251         5349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1000538, whole genome shotgun sequence.
ACCESSION   DASPGK010000251 DASPGK010000000
VERSION     DASPGK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000538"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VEC56_06815"
     CDS             <1..676
                     /locus_tag="VEC56_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXV13902.1"
                     /translation="THFRLPVVLENDATSAAFGESFVRGERGRDLVFITLGTGVGGGI
                     VADGKVVRGAAGFGGEIGHMTVDPNGPRCRCGSRGCLEAYAGAYGILRAARAAAKSRG
                     RSRSPLARRLAGRGVTARDVMDAARRGDGVGREAARVAGEHLGLAIASMLNVLNPSVV
                     VIGGGVAGGFDVLEPHARRTVARFAFPDAVRAARIERSRLGNDAAVVGAAMLARLGTR
                     PARKTR"
     gene            673..4224
                     /locus_tag="VEC56_06820"
     CDS             673..4224
                     /locus_tag="VEC56_06820"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HXV13903.1"
                     /translation="MSRLRLFGALASAAALACVLGSLPGRAQIIPPLPSAAYYDVSAD
                     HSQLKKVGEETVLELTGNVRVVHGDVTVTADRGLSFTTQRLTQLFGNVRAVQQTMVMT
                     GAEGEYRQLEDLAIVRKNVRIVDEGWEVTCNEARYSRVKDEAWLVGNVVGKDSTSTIR
                     ADRVLYKRGLGLAEAFGAVEISDGAQDIIVRGKHGLFYRDRSEGVVDREPVLVSGPDD
                     PEPITVVSDTMRVFPDSSRATAYYRVKIIKGDMVTQCDSAMLYDDQQRVELYGNPLAK
                     QDNVAMKGERMVAHYNEDEVYQIDILDAASITETQRDSLVAGRDSWIQGDAITLYLHD
                     GGVDSVRVLGKAQSEYYPKNPGKVEGNVIEGEDMFFHFGEDEIDFVDVKGGASGTYRY
                     INLGNSETPDSMRAVLDTTLTYVAFPEKAEKVVYAAERIRYYADREDLVLDEKARVDY
                     KDSRLEAANITYHASVQVLDATGSPTLTDAGQSIVGEQMDYDLEGETGLVIEGSTQYE
                     QGYYSGENMAKVGENEMKVWNSWYTTCDLKEPHYHFGAKHMKVYPDDKAFTGPIWLHI
                     GKTPIFALPFMANSIARGRRSGFLRPDFEFGITSDRGRFISGVGYYWATNDYTDFTFA
                     GDFYEDSRWRLYVGNRYALRYKFNGSAAYNYVRDITDNSTEWTFDGTHNQTLGERFTL
                     NASLRFVSSDDAPQSVNQIDDVNRYIDRSIRSTVSLRKSWETVGFSASASRTQNLSIT
                     DPTALKLSSTLPSVQLSIPSWNLYFGNESRTADGVWENLLRNTRVSPSLSFNRAVREK
                     LFETDDVMEARAQAGLSSPQKFGFVTISPSISGDLTSTRVDFQRDAHQYYRTVGSVTD
                     TITVSALDSSLTEHQFTWRTGVGANTNIYGTFYPNIGRLRGLRHTLTPAVNYSFTPSQ
                     DGRPRSQSVSLGLRNAIDLKVAPAVVDTTTSEEEKVRRLSGVVLWSLSTSYRPDTPSE
                     RAWGNISSGLNFMLLGTNFSINHSIDPYRFDVLSTSATSTIRLGGTHPFGTSSRVEVR
                     ELNVAAARDTTRAKKEEFESGGVEFRQTGEYGERPADELQLEEGRQPWNVVLGFTYSK
                     GASGVASSTLRVGWDVKLTDNWRIDYSTIYDIEGRERSGQYIGVTRDLHCWEIGFSRQ
                     QLGDEWKYYFRIALKAHPELYGESGDRGVGGGLMGHF"
     gene            4357..4650
                     /locus_tag="VEC56_06825"
     CDS             4357..4650
                     /locus_tag="VEC56_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV13904.1"
                     /translation="MNKTELIERVAQKTGLTMKDAKNVVDAIFSTQPKNGIIANELKS
                     GRKVQITGFGTFQIRRRKKREGRNPQTGATIEIPATKFPAFSAGRALKDRVKV"
     gene            4793..>5349
                     /locus_tag="VEC56_06830"
     CDS             4793..>5349
                     /locus_tag="VEC56_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13905.1"
                     /translation="MRLWTGIATVFAVALGMGAAQAPPSDPPKPNPAVATPNAPWQPD
                     VRLVYESDTRAYYRPCGCNQNSAGGLARRAALLKDFRAQSAPVLVCTAGDFYGTADIF
                     NEAKSRFVARMMDDLGYDAVGVGEMDLNFGLATLVADVRALGLPVVCANLRSQTDSLR
                     VRGDGPAAAAAARLGSAFPPYRVVT"
BASE COUNT          954 a         1801 c         1765 g          829 t
ORIGIN      
        1 cacccatttt cgcctcccgg tcgtgctcga gaacgacgcc accagtgctg ccttcgggga
       61 atcgttcgtg cgcggcgagc gcgggcgcga tctggtgttc ataacgctgg gaaccggggt
      121 aggaggcggc atcgttgccg acgggaaggt ggtgcgaggg gcggctgggt tcggcggcga
      181 gatcggtcac atgaccgtcg accccaacgg ccccagatgc cggtgcggat cgcgcggctg
      241 cctggaagcg tacgcgggcg cctatggaat cctgcgcgcc gcgcgcgcgg ccgcgaagtc
      301 gcgcggtcgc tcgcgaagcc cgctcgcgcg gcgtctcgcc gggcgcggcg tcaccgcccg
      361 ggatgtaatg gatgccgccc gccgcggcga cggggtcggg cgcgaagcgg cccgcgtggc
      421 cggcgagcac cttggcctgg cgattgcgtc gatgctgaac gtgctcaacc catccgtggt
      481 cgtgatcggc ggcggggtgg cgggcggctt cgacgtcttg gaaccgcacg cgcgccgcac
      541 cgtcgcgcgc ttcgccttcc ccgacgcggt gcgcgccgcg cgcatcgagc gctcgcggct
      601 gggcaacgac gccgcggtgg tcggcgcggc catgctcgcg cgactcggca cccgccccgc
      661 aaggaagacc cggtgagtcg actcagactt ttcggagcgc tcgcgtcggc cgcggctctc
      721 gcgtgcgtgc tcggctcgct ccccgggcgc gcgcagatca tcccgcccct accgtccgcc
      781 gcctactacg acgtcagcgc cgaccacagc cagctcaaga aagtcggcga agagaccgtg
      841 ctcgaactca cgggcaacgt gcgcgtcgtc cacggcgacg tgaccgtgac cgccgaccgc
      901 ggcttgagct tcaccaccca gcgcctcacc cagctcttcg gcaacgtgcg cgcggtgcag
      961 cagaccatgg tcatgaccgg cgcggagggc gagtaccgcc agctcgaaga cctcgcgatc
     1021 gtgcgcaaga acgtgcgcat tgtggacgag ggctgggaag tgacgtgcaa cgaggcgcgt
     1081 tattcgcgcg tcaaggacga ggcctggctc gtcgggaacg tggtcggcaa ggactccacc
     1141 tcgaccatcc gcgccgaccg cgtgctctac aagcgcggcc tcgggctggc ggaggcgttc
     1201 ggcgcggtcg agatctccga cggcgcgcaa gacatcatcg tgcgcggcaa gcacgggctg
     1261 ttctaccgcg atcgcagcga gggtgtcgtc gaccgcgagc ccgtgctcgt ctccggtccc
     1321 gacgaccccg agcccatcac cgtggtcagc gacaccatgc gcgtgttccc cgactcctcg
     1381 cgcgccaccg cgtactaccg cgtcaagatc atcaagggcg acatggtgac ccagtgcgac
     1441 tccgccatgc tctacgacga ccagcagcgc gtcgagctct acggcaaccc cctcgccaag
     1501 caggacaacg tggccatgaa gggcgagcgc atggtggcgc actacaacga agacgaggtc
     1561 tatcaaatcg acattctgga cgccgcgtcg atcacggaga cgcagcgcga ctcgttggtg
     1621 gcggggcgcg acagctggat ccagggcgac gccatcacgc tctatctgca cgacggcggc
     1681 gtggactcgg tgcgcgtgct gggcaaggcg caaagcgagt actaccccaa gaatcccggc
     1741 aaggtggaag gcaacgtcat cgaaggcgaa gacatgttct tccacttcgg cgaagacgag
     1801 atcgacttcg tcgacgtcaa gggcggcgcg tcggggacct accgctacat caacctcggg
     1861 aactccgaga cgcccgacag catgcgcgcc gtgctcgaca ccacgctcac ctacgttgcg
     1921 ttcccggaga aggcggagaa ggtggtgtac gccgccgagc gcatccgcta ctacgccgac
     1981 cgggaggacc tcgtgctcga cgagaaggcg cgcgtcgact acaaggacag ccggctcgaa
     2041 gccgccaaca tcacctacca cgcgtcggtg caggtgctcg acgccaccgg ctcacccacc
     2101 ctgaccgacg ccggccagtc gatcgtcgga gagcaaatgg actacgacct cgagggcgag
     2161 accgggctcg tgatcgaggg gtccacgcag tacgagcagg gctactacag cggcgagaac
     2221 atggccaagg tgggcgagaa cgagatgaag gtgtggaact cgtggtacac gacctgcgac
     2281 ttgaaggagc cgcactatca cttcggggcc aagcacatga aggtgtaccc ggacgacaag
     2341 gccttcacgg gtccgatctg gctgcacatc ggcaagacgc ccatcttcgc gctgccgttc
     2401 atggccaact cgatcgcgcg cggacggcgc tcgggattcc ttcgccccga cttcgagttc
     2461 ggcatcacca gcgaccgggg gcgcttcatc agcggtgtgg gctactactg ggcgaccaac
     2521 gattacaccg actttacgtt cgccggcgac ttctacgaag actcgcgctg gcgcctgtac
     2581 gtgggcaacc gctacgcgct gcgctacaag ttcaacgggt cggccgcgta caactacgtg
     2641 cgcgacatca ccgacaattc gaccgagtgg accttcgacg gcacgcacaa ccagaccctg
     2701 ggcgagcgct tcacgctcaa cgcgagcctg cgcttcgtgt cgagcgacga cgcgccccag
     2761 agcgtcaacc agatcgacga cgtcaatcgc tatatcgacc ggtcgatccg ctcgacggtg
     2821 tcgctgcgca agagctggga gacggtcggt ttttccgcgt cggccagccg cacgcagaac
     2881 ctgagcatca ccgacccgac cgcgctgaag ctcagctcca cgctgcccag cgtgcagctg
     2941 tcgattccct cgtggaacct gtacttcgga aacgagagcc ggaccgcgga cggcgtgtgg
     3001 gagaacctgc tgcggaatac gcgcgtgtcg ccgtcgctgt cattcaatcg ggcggtgcgc
     3061 gagaagctgt tcgagaccga cgacgtcatg gaagcgcgcg cgcaggcggg actctccagc
     3121 ccgcaaaagt tcggcttcgt gaccatctcg ccctccatct cgggcgacct gacctcgacg
     3181 cgggtcgact tccagcgcga cgcccaccag tactaccgga ccgtgggcag cgtgaccgac
     3241 accatcaccg tgtcggcgct cgattcgtcg ctaacagagc atcagttcac gtggcgcacg
     3301 ggcgtggggg cgaacaccaa catctacggc acgttctacc ccaacatcgg gcgcctgcgc
     3361 ggccttcgtc acaccctcac gccggccgtg aactactcct tcaccccgtc ccaggacgga
     3421 cggccgcggt cgcagtcggt gtcgctgggg cttcgcaacg cgatcgacct caaggtcgct
     3481 cccgccgtgg tggacacgac cacgagcgag gaggaaaagg tgcgtcgtct ctcgggcgtg
     3541 gtcttgtgga gcctgtcgac gtcctaccgg cccgacaccc ccagcgagcg cgcgtggggc
     3601 aacatcagca gcgggctgaa cttcatgctc ctcggcacca atttctccat caatcacagc
     3661 atcgacccgt accgcttcga cgtcttgtcg accagcgcca cctccaccat ccgcctgggc
     3721 ggcacccatc cgttcgggac atcgtcgcgc gtggaagtgc gcgagctcaa cgtggcggcg
     3781 gcgcgcgaca ccacgcgcgc gaaaaaagag gagttcgaat cgggcggggt ggagtttcgc
     3841 cagaccggcg agtacggtga gcgccccgcc gacgagctcc agctcgaaga gggccggcag
     3901 ccgtggaacg tcgtgctcgg tttcacatac tcgaagggcg cgtccggcgt cgccagctcg
     3961 accttgcgcg tcggctggga cgtcaagctg accgacaatt ggcgcatcga ctactcgacc
     4021 atctacgaca tcgaggggcg ggaacggtcc gggcagtaca tcggcgtcac gcgcgacctc
     4081 cactgctggg agatcggctt ctcgcgccag caactcggtg acgagtggaa gtattacttc
     4141 cgcatcgcgc tcaaggccca ccccgagttg tacggcgaat cgggcgatcg cggggtcggg
     4201 ggcggtctca tggggcattt ctagggtatg cgcccaaggc tggaaggaga tatcccccga
     4261 aacccgcgtg ggcacgccac gaaggccgga atatgtgttg acacgttttt ttcgaggtcg
     4321 atagagtgcg cgtgagaccg ggaaggaggt gacagaatga acaaaaccga attgatcgaa
     4381 cgcgtagcgc agaagacggg gctcacgatg aaagatgcca agaacgtcgt cgacgcgatt
     4441 ttcagcacac agcccaagaa cggcatcatc gccaacgagc tcaagtcggg tcgcaaggtt
     4501 cagatcaccg gcttcgggac attccagatc cgcaggcgca agaagcgcga gggtcggaat
     4561 ccgcagaccg gcgcgaccat cgagattcca gcgaccaagt tcccagcctt ctccgcgggc
     4621 cgcgcattga aggaccgcgt caaggtgtag ggacccggca gtttcgaaac tgctcggcag
     4681 gcggagcgca cgacagcgcc ccgcctgctt tttttgtctc cggcggcggt cctgcctgcc
     4741 ctgactggcc gacggacgcg gtgccggcgg atggtgtata ttgagcgtcg tcatgcgact
     4801 ctggaccggc atcgccactg tgttcgcggt cgcgctcggg atgggcgcgg cgcaggcgcc
     4861 gccgtcggac ccccccaagc ccaacccggc cgtcgccacc ccgaacgccc cctggcagcc
     4921 cgacgtccgc ttggtctacg agagcgacac tcgcgcgtac taccgtccgt gcgggtgcaa
     4981 ccagaactcg gccggcgggt tggcgcggcg ggcggcgctt ttgaaggact ttcgtgccca
     5041 atccgccccc gtgctcgtgt gcaccgcggg tgacttctac ggaaccgccg atatcttcaa
     5101 cgaggccaag tcgcgcttcg tcgcgcgcat gatggacgat ctcggttacg acgcggtcgg
     5161 ggtgggggag atggacctca acttcgggtt ggcgacactg gtcgccgacg tgcgcgcgct
     5221 ggggcttccg gtggtttgtg cgaacctccg ctcccaaacg gactcgctgc gcgtgcgcgg
     5281 cgacggcccg gccgcggccg ccgcggctcg gctcggctcc gcgtttccgc cctaccgggt
     5341 ggtgaccaa
//
LOCUS       DASPGK010000252         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1118640, whole genome shotgun sequence.
ACCESSION   DASPGK010000252 DASPGK010000000
VERSION     DASPGK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118640"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VEC56_06835"
     CDS             complement(<1..317)
                     /locus_tag="VEC56_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13906.1"
                     /translation="MKKLLVAFASLSALSMSSTAAFADAKPINLSLFTPVSIAKETDS
                     VTAFRFNLLYGKNTSVEVLDLGLVNHTTSGLSKGLAWGLVNMAEADFKGLQIATVNYN
                     AGS"
     gene            591..1349
                     /locus_tag="VEC56_06840"
     CDS             591..1349
                     /locus_tag="VEC56_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13907.1"
                     /translation="MCPVRTLAMPAVCLVAGLAMGCATRWTPPDIPHQPADTTALGVR
                     DELPPLAMWPGEARRIALPVVVTTPKSSRVFVLRGDPALAFRLDLPKTAFVPILQGAL
                     PAPSAQGACIVRVDSLATPGDTLSFHYTVSTTTRPPRFSRWRGSVVVEMPLLVIDGLG
                     GASNTVSVGPNPFNPETTVHYVVTSPGRVTLEVYDVRGARIATLVDAKLAAGEHTATW
                     NGRDEKGVPVGSGVYFGRLTSPAGVRSYKMTVVK"
     gene            complement(1363..3792)
                     /locus_tag="VEC56_06845"
     CDS             complement(1363..3792)
                     /locus_tag="VEC56_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13908.1"
                     /translation="MPEHPRLCPRPSLGALFLASALLACLSVPVHAVELWGTDGDVAA
                     LAASGNLLYVGGNFRTLAPPTGALVAVDFATGTPTLGLGGITAVTAAAGGVQAAAPDG
                     AGGWYVGGTFNIAGGQLHQNLAHVLADGSVDHAWAPSANNSVRAIAVIGSTVYIGGDF
                     TMVTSTARNRLAALDATTGALGGWNPGANATVNCLLANGANLYVGGLFTMVNAILRRN
                     IAEVDASTGVPTAWDPNGNGRVRTMVLSGPSLYVGGAFSNIGTQNRNRVAALDVTTGV
                     ASSWNPNANGDVYSIVVNGPTVYVSGEFTAVGGSNRAYIGAVNNDAFGGSTTWNPSAS
                     NPVYGIVLNGSTIYAGGAFTTMQLQPRQRLAAIDAATGTPTAWTSHATGTVWMLGSNG
                     SSLVAGGTFMGVGGVLRNSLAALDLTSGIPTSWNPGANSWVYAVIPNGATVLVGGSFS
                     SFGGQPRSNLVEVDAASGSLTGWDFGATSAVQVMRIDGNTLYVGGLFTSIGGALRDRI
                     AAVDLTTQTPTAWNPGANGSVFDILPAGGVVYAAGSFTTIGGAAHNRVAALDPSSNSA
                     LPFSADVNNVALTLALDGSTLYCGGQFTGSGVQPRNRCAAFDATSGNLLTWAPICGSS
                     NVDDVVVAGGDIYLGGHFFSLNGANTRPYLGAVDAAGATVADFDPRAGGPVLAMVTVG
                     NNLCVGGAFGYMAWEPYSNLALLAIGPPVAVENRAPALIAPRIHPNPTSAGATITFSQ
                     SVTGETAVEIFDVAGRFVRTLHTGRLPAGPHQLAWDGRDERGKRAPTGVYFVRVETAG
                     QTVATKLVLVR"
     gene            3975..4286
                     /locus_tag="VEC56_06850"
     CDS             3975..4286
                     /locus_tag="VEC56_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13909.1"
                     /translation="MYGRKVMFELKPNSLNDFNRRQEAEIVPILRKQKGFQDVITLVA
                     PDKKRIEAISMWDRKEDAEAYDLSGYKDVVKLLATVVEGTPKVETMEMASSTIHKAGA
                     R"
     gene            complement(4344..>4536)
                     /locus_tag="VEC56_06855"
     CDS             complement(4344..>4536)
                     /locus_tag="VEC56_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008867715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HXV13910.1"
                     /translation="GTFASLEELPGREISQVPHPLMSETRALLEGTRAKLWFIHLNHS
                     NPAIVEGKDVAREGMRFQL"
BASE COUNT          779 a         1518 c         1520 g          719 t
ORIGIN      
        1 aagctgcccg cgttgtaatt gacggtcgcg atctgcaggc ccttgaagtc cgcctcggcc
       61 atgttgacga ggccccacgc gagacccttc gagagacccg acgtggtgtg attgacgagc
      121 ccgaggtcga gcacttcgac cgacgtgttc ttgccgtata gcaggttgaa gcgaaacgct
      181 gtcaccgagt cggtctcttt cgcgatcgac accggcgtga acaatgagag attgatcggc
      241 ttcgcgtccg cgaacgcggc cgtcgacgac atcgacagcg cagacaacga ggcgaacgcg
      301 acaagcagct tcttcatgag agcgctcctt tgaggataga ggaacgagta gtctacccgg
      361 ccgcccgcgg gttgtccacc ctgcgaagcg catcggaccc cggcgccgcg caccgacgcc
      421 ggttcgaggc ggccccgcca gacgggcttt ccgagcccct tgcgcgcggg cgtttcgtcc
      481 gccatctttc cgtcgtcccc acttcatact tccacgaccc aaggtacccc acgtcatagc
      541 gaagagggcc ggcggcgacg gattgcgtcg cggctgggaa ggagcgcgtc gtgtgccctg
      601 tgcgcacgct cgcgatgccc gcggtgtgtc ttgtcgcggg tctcgcgatg ggctgtgcaa
      661 cccgctggac cccacccgat attcctcacc aacccgccga caccaccgcg ctcggcgtgc
      721 gcgacgagct gccgccgctc gcgatgtggc cgggcgaagc gcgccgcatc gcgctgcccg
      781 tggtggtgac gacgccgaag tcgtcgcgcg tgttcgtcct gcgcggcgac cccgcgctcg
      841 cattccggct cgatcttccc aagaccgcct tcgtgcccat tctccagggc gcgctgcccg
      901 cgccgagcgc gcagggtgcg tgcatcgtgc gggtcgattc gctcgccacc cccggcgaca
      961 cgctctcgtt ccactacacg gtctcgacca cgacccggcc gccgcgcttc tcgcgctggc
     1021 gagggagcgt cgtcgtggag atgccgctgc ttgtcataga cggactcgga ggtgccagta
     1081 acacggtcag cgtcggcccg aatcccttca accccgagac gacggtgcac tacgtcgtca
     1141 catcaccggg ccgcgtgacg ctcgaggtct acgacgtgcg cggcgcgcgc atcgccacac
     1201 tcgttgacgc gaagctggcg gcgggagagc acacggcgac ctggaacggc cgcgacgaaa
     1261 agggagtgcc ggtgggctcg ggggtgtact tcggacgact gacgtctccc gccggagtca
     1321 ggagctacaa gatgacggtg gtgaaatagc ggcccaggcc tgtcaacgaa ccagaaccag
     1381 cttggtcgct acggtctgcc ccgccgtctc gacgcggacg aagtagacgc ccgtcggagc
     1441 gcgcttgccg cgctcgtcgc ggccgtccca cgccagctgg tgcgggcccg cgggcagacg
     1501 tcccgtgtgc agagtgcgca cgaaccggcc cgcgacgtcg aagatctcca cggcggtctc
     1561 gcccgtgacg gactgcgaga aggtgatcgt cgcgcccgcc gaggtcgggt tgggatggat
     1621 gcgcggcgcg atcagcgcgg gcgcgcgatt ctccactgca accggcggcc cgatcgcgag
     1681 tagcgcgagg ttgctgtacg gctcccacgc catgtagccg aacgcgccgc ccacgcacag
     1741 gttgttgccg acggtcacca tcgcgagcac gggaccgccc gcgcgcgggt cgaaatcggc
     1801 caccgtcgcg cccgccgcgt cgaccgcgcc cagatacggg cgcgtgttcg cgccgttcag
     1861 actgaagaag tgcccgccga gatagatgtc gccgcccgcg acgacgacgt catcgacgtt
     1921 gctgctcccg caaatcggcg cccacgtgag caagttgccc gaggttgcgt cgaacgcggc
     1981 gcaccgattg cgcggctgga cgccggaccc ggtgaactgg ccgccgcaat acaacgtcga
     2041 gccgtcgagc gcgagggtca acgccacgtt gttgacgtcg gcggagaagg gcagtgcgct
     2101 attgctggat ggatccagcg cggccacgcg gttgtgggcc gcgccgccga tggtcgtgaa
     2161 cgagccggcc gcgtacacga caccaccggc cgggaggatg tcgaacaccg acccgttcgc
     2221 gcccggattc cacgccgtcg gcgtttgggt ggtgaggtcg acagcggcga tgcggtcgcg
     2281 cagcgctccg ccgatcgagg tgaagaggcc gcccacgtag agcgtgttgc cgtcgatccg
     2341 catcacttgg acggcgctgg tggccccgaa atcccagccg gtcaacgaac cgctcgcggc
     2401 gtccacttcg acgagattgc tgcgcggttg tccaccgaag ctcgagaagc tcccgcccac
     2461 gaggacggtc gcgccgttgg gaatgaccgc gtacacccac gaattcgcgc ccgggttcca
     2521 cgaggtcgga atgccgctgg tgagatccag cgcggcgagg ctgttgcgaa gcacaccgcc
     2581 cacgcccatg aaggtcccgc ccgcgacgag cgacgagccg ttcgaaccca gcatccacac
     2641 cgtgccggtc gcgtgcgacg tccacgcggt cggcgttccc gtggccgcgt cgatggccgc
     2701 caggcgctga cgcggttgca gctgcatggt cgtgaacgcg ccacccgcgt agatcgtgga
     2761 gccgttgagc acgattccat acaccgggtt gctggcgctc ggattccacg tcgtcgaccc
     2821 gccgaaggcg tcgttgttga cggcaccgat gtacgcccgg ttcgaaccac ccacggccgt
     2881 gaattcgccc gacacgtaaa ccgtcggacc gttgacgacg atcgagtaga cgtcgccgtt
     2941 cgcgttcggg ttccacgagc tcgcaacccc cgtcgtgacg tcgagcgccg cgacgcggtt
     3001 gcgattctgg gtgccgatat tggagaaggc accgcccaca tacaatgacg gaccgctcaa
     3061 caccatcgtg cgcacgcgtc cgttgccgtt ggggtcccac gcggttggga cgccggtcga
     3121 cgcgtcgacc tcggcgatgt tccggcgcaa gatggcgttg accatcgtga acaatccacc
     3181 gacgtagagg ttggcgccgt tggcgagcag gcaattcacg gtcgcgttgg cgccgggatt
     3241 ccacccaccg agcgcgccgg tggtcgcgtc caacgcagcc agccggttgc gcgcggtgct
     3301 ggtcaccatg gtgaagtcgc cgccgatgta cacggtcgag ccgatcacgg cgatcgcgcg
     3361 cacgctgttg ttcgcacttg gcgcccaggc gtggtcgacc gacccgtcgg cgagcacgtg
     3421 ggcgagattc tggtgcagtt gaccgcccgc gatattgaac gtgccgccca cataccaccc
     3481 acccgctccg tcgggcgccg cggcttgcac accgccggcc gccgccgtaa ccgccgtgat
     3541 cccgccgagc ccaagcgtgg gtgtcccggt cgcgaagtcg accgccacca gcgcgccggt
     3601 tgggggggcg agtgtgcgaa agttgccgcc cacgtagagg aggttgcccg aggcggcgag
     3661 ggcagcgacg tcaccgtcgg tgccccacag ttcgaccgcg tggacgggaa cggagaggca
     3721 agccaagagg gcgctggcga ggaagagggc gccgagcgaa gggcgggggc aaaggcgcgg
     3781 atgttcgggc acgtcgggtc tccaggtcct gcggcgccat ccagtttaca ccttttccgg
     3841 tccgaacgaa acgccgtcgt cacgacggtc gcggcccgcg cagaatgatg caagtgcatc
     3901 atggcggggg cgcggacgca gggctaagtt acaaatgaac cacttcaatt cgcaattcat
     3961 ggaggaggag aatcatgtac ggacgtaagg ttatgttcga gttgaagccg aacagcctca
     4021 acgatttcaa tcgtcgtcag gaagccgaga tcgtcccgat cctgcgcaaa cagaagggct
     4081 tccaggatgt catcaccctg gtagcgcccg acaagaagcg catcgaggcg atcagcatgt
     4141 gggaccggaa ggaagacgcg gaagcgtacg atctctccgg ctataaggat gtcgtcaagc
     4201 tgttggcgac agtcgtggaa gggacgccga aggtcgagac catggaaatg gcctcatcga
     4261 cgatccacaa ggccggcgca cgctaagccg ccgttctttc caggtaccca atcggggggc
     4321 gggccggggc ccgccccctt tttctaaagc tggaaccgca tcccttcgcg cgccacgtct
     4381 tttccctcca cgatggccgg gttcgaatgg ttcaggtgga tgaaccacag tttggcgcgc
     4441 gtaccttcga gcagcgcgcg cgtttcgctc atcaggggat gcgggacctg cgagatctcg
     4501 cgccccggca attcttcgag tgacgcgaac gtccca
//
LOCUS       DASPGK010000253         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_300391, whole genome shotgun sequence.
ACCESSION   DASPGK010000253 DASPGK010000000
VERSION     DASPGK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300391"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VEC56_06860"
     CDS             <1..601
                     /locus_tag="VEC56_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13911.1"
                     /translation="SDGFTTGLSPAAGGLYVASFDAVSDKVVHRYDALHRWVSSFSDS
                     WSAVESMPFDVERWCNGGAIDVGPDGSVYYTQWTPYEIRKFTPDGKLRLTIRRENDFK
                     PPRVERQADSVAFYNYTGSSAILVLSDGRILNVVSVVADDHKIVGTLLELFDADGRFL
                     KSIKVDRLVGIKCRDARDRIYAWEARVVPQVVRYRLGFR"
     gene            694..1308
                     /locus_tag="VEC56_06865"
     CDS             694..1308
                     /locus_tag="VEC56_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13912.1"
                     /translation="MTRTSLVMVAMAAIGLPILAVASIPDPCTSTVGSAAGVLVACPR
                     GDGPSLGSLGLTITIEVRDPTNVPVPNIPATDIWLIGCGDLLALCGGTGAINASGPTD
                     AAGRTTITDSFAAGGCDRGVLVVVQGIIIDGPGCGQPCPPIAVRSPDINASLAVNVAD
                     LALFGAGYPSPPQAYGECLDFAAPYGAINLADFSKFGEHYQHLC"
     gene            1385..2923
                     /locus_tag="VEC56_06870"
     CDS             1385..2923
                     /locus_tag="VEC56_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXV13913.1"
                     /translation="MHTTVFSGAVVGIQGLVIEVEVDIARGIPAFSVVGLPNAAVRES
                     RERVTAALKNAGWQFPLERITVNLAPADVKKEGSAYDLAIAIGILLASGQAEATTTRT
                     LFIGELALDGSLRKVPGVLPILLHARAQGFARAVVPLANLEETRVVEGVEVFACESLR
                     AAVSVALASPDSEFDAGIDGRVVVKRAEDPVPSPRAAASSSLDMADVVGQEAVKRALL
                     IAAAGGHHVLMTGPPGAGKTMLARRFAGILPPLSSERAIETTMIYSIVGLLSNGGLVT
                     RAPFRAPHHSASDAGLIGGGGVPRPGEVTLAHNGVLFLDELPEFRRHVLETLREPLEE
                     GSITISRARSAVTFPARFQLVGAMNPCHCGMHGSKLRQCRCSPLQIESYLAKISGPLL
                     DRIAIHVTVQPVDVDMWGSYSNKMSSAEMRDLVIRARDIQARRFEGLDGVDCNARLPE
                     GMFAAFCAMEPGAETLFLRAQKSLLVSARARGHIVRVARTIADLEGKDDIAERHIGEA
                     VGYR"
     gene            complement(2980..3669)
                     /locus_tag="VEC56_06875"
     CDS             complement(2980..3669)
                     /locus_tag="VEC56_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV13914.1"
                     /translation="MRTYGQYCPIARGAEIFAERWTPLIIRNLHLGSGSFSEILEGAP
                     GLSRTLLSQRLQQLERVGIVELAPKSDGRGHHYALTSAGRELFTVCQSLGEWGARWLE
                     IAPENLDPFVALWSMCHALRRDRLPDRRVVIRFDFTGRSRRESYWLLIELGDTEICRT
                     HPGFDEDLYVTAEAEAFVKWHAGQLTWAQAIREDRIQLDGPSWLIKAFPTWNARSMFA
                     HIRTVHPPRLP"
     gene            3773..>4442
                     /locus_tag="VEC56_06880"
     CDS             3773..>4442
                     /locus_tag="VEC56_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV13915.1"
                     /translation="MNTTKTTKVGRGSTKPTVAGIDGYRGRLIGPDHADYDVARAVWN
                     GSIDRRPRLIARCIGAADVVAAVRFAREHDLEIALRGGGHNVAGTAVCDDGIVIDLSA
                     MRAVRVDPADRRVWVQGGALWGDVDHETQAHGLATTGGIVSHTGVAGLTLGGGVGWLM
                     RKHGLTVDNLLAVDVVTADGGRLRASEQEHPDLFWALRGGGGNFGVVTSFEFCLHPVG
                     PAVLA"
BASE COUNT          718 a         1414 c         1521 g          789 t
ORIGIN      
        1 ctccgacggg ttcaccacgg ggttgagtcc cgccgccggc gggctctacg tggccagctt
       61 cgacgccgtg agcgacaagg tcgtgcaccg ctacgacgcg ctgcatcgct gggtgtcgtc
      121 gttctccgat tcgtggagtg cggtcgagtc gatgccgttc gacgtggagc gctggtgcaa
      181 cggcggtgcg atcgacgtcg gcccggacgg gagcgtctac tacacgcagt ggacgcccta
      241 cgagattcgc aagttcaccc ccgacggaaa gctccgactc acgattcgtc gcgagaacga
      301 cttcaagccg ccgcgcgtcg agcgccaagc cgactccgtg gccttctaca actataccgg
      361 ctcgtccgca attcttgttc tgtcggacgg gcgcattttg aatgtggtca gcgtggtcgc
      421 ggacgaccac aagatcgtgg gcacgttgct cgagctgttc gacgccgacg gccggttctt
      481 gaaatcgatc aaggtggacc gcttggtcgg aatcaagtgc cgcgacgcgc gcgatcgaat
      541 ctacgcctgg gaggcgcgcg tggtgccaca ggtggtgagg tatcggttgg ggtttcgtta
      601 gcgcccaggt ggtaaggaaa acgagttttc ttgttctctc ggcgtggttc tcggaccccg
      661 agacgcgtcc aactcattga aggaggtcga atcatgacaa gaacaagtct ggtgatggtg
      721 gcgatggcgg cgattggctt gccgattctc gcggttgcga gcatccccga tccgtgcaca
      781 tccaccgtcg gctccgcggc tggcgttctg gtcgcgtgtc cgcggggcga cggcccgtcc
      841 ttgggctcgc tgggtctcac gatcaccatc gaggtccggg acccgacgaa cgtgcccgtt
      901 ccgaacattc cggcgaccga catctggttg atcgggtgcg gcgacctgtt ggcgctgtgc
      961 gggggtacgg gcgcgatcaa cgcctcgggg cccaccgacg ccgcggggcg caccacgatc
     1021 acggattcgt ttgccgcggg cggttgcgac cgcggcgtgc tcgtggtcgt gcagggaatc
     1081 atcatcgacg gccccggttg cggacagccg tgcccgccca tcgccgtccg gtcgcccgac
     1141 atcaacgcct cactggccgt caacgtcgcc gacctcgcgc tcttcggagc tgggtacccg
     1201 tcgccgccgc aggcctacgg cgaatgcctc gacttcgcgg cgccctacgg cgcgatcaat
     1261 ctggccgact tctcgaagtt cggagagcac taccagcact tgtgctaggt cgcgcctggt
     1321 cgtgatagaa tccccgcctc ctctcgggtg atgcagttgg gttgggttcg tgtggaggtg
     1381 ggggatgcat accacggtct tctccggcgc cgtcgtcgga atccagggtc tcgtcatcga
     1441 agtggaagtc gacatcgcgc gcgggatccc cgcgttctcg gtggtcggtt taccgaatgc
     1501 cgcggttcgt gagagccgcg agcgcgtcac cgcggcgctc aagaacgcgg gctggcagtt
     1561 tccgctcgaa cgaatcaccg tcaacctcgc gcccgcggac gtcaagaaag agggctccgc
     1621 gtacgacctc gccatcgcga tcggtatcct gctcgcttcc gggcaggccg aagcgaccac
     1681 gacgcgcacg ctcttcattg gcgagctcgc cctcgacggg tcgttgcgca aggtccccgg
     1741 tgttcttccc attctgttgc acgcacgcgc gcagggcttc gcgcgcgccg tcgtgccgct
     1801 cgccaacctg gaagaaaccc gtgtcgtcga aggcgtcgaa gtcttcgcgt gcgaatccct
     1861 gcgcgccgcc gtgtcggtcg cactcgcgtc gccggactcc gaattcgacg cgggcatcga
     1921 cgggcgcgtg gtcgtcaagc gcgcggagga tcccgtgccc tcgccgcgtg cggccgcgtc
     1981 gtcgtcgctc gacatggccg acgtcgtggg gcaggaagcg gtcaagcgcg cgctcctcat
     2041 agcggcggcg ggcgggcacc atgttctgat gacgggtcct ccgggtgcgg gcaagaccat
     2101 gctcgcgcgc cgcttcgcgg ggattctgcc gccgctctcc tcggagcgcg cgatcgagac
     2161 cacgatgatc tacagcatcg tcggcttgct ctcgaacggc gggctggtga cgcgcgcccc
     2221 cttccgcgcg ccgcaccaca gtgccagcga cgccgggttg atcggcggcg gcggtgtgcc
     2281 gcgtccgggc gaagtgacac tcgcgcacaa cggggttctt tttctcgatg aactaccgga
     2341 gtttcggcgc cacgttctgg aaaccctgcg cgagccgctc gaagaaggat cgatcacgat
     2401 ctctcgcgcg cgcagcgccg ttacctttcc cgcccgattc caactggtcg gcgcgatgaa
     2461 cccctgtcac tgcggcatgc acgggagcaa actaagacaa tgtcgttgca gcccgcttca
     2521 gatcgagtcc tacttggcga agatctcggg cccgctgctc gatcgcattg ccatccatgt
     2581 cacggtgcaa cccgtggacg ttgatatgtg ggggtcatat tctaacaaaa tgagcagcgc
     2641 cgagatgcgg gacctggtga ttcgggcacg agacatccaa gcgcggcgct ttgaggggct
     2701 ggacggcgtc gactgcaatg cgaggcttcc cgaagggatg ttcgcggcct tctgcgccat
     2761 ggagccgggc gccgagacgt tgttcctccg cgcacagaaa tcactgctcg tcagcgcgcg
     2821 tgcgcgaggc cacattgttc gcgtcgcacg cacgatcgca gacctggagg gaaaggacga
     2881 cattgcggaa cggcacatcg gggaggcggt gggatatcgg tgagacgcag atgatgggag
     2941 cggggcatcc cggtcaccgg ccgatccacc agcgcgacct caaggaagcc gcggagggtg
     3001 aaccgtccga atgtgggcga acatgctgcg ggcgttccag gtagggaagg cctttatgag
     3061 ccacgagggg ccgtcgagtt ggatgcggtc ctcgcggatg gcttgggccc acgtgagttg
     3121 gccggcgtgc catttgacga aggcttcggc ttccgccgtg acgtagaggt cctcgtcgaa
     3181 accggggtgc gtcctgcaaa tctcggtgtc tccgagttcg atgagtagcc agtagctttc
     3241 acggcgcgat cggccggtga agtcgaagcg gatgacgacg cgtcgatccg ggagccggtc
     3301 tcggcggagt gcgtggcaca tcgaccacag agccacaaac gggtcgaggt tctccggggc
     3361 gatctccagc caacgcgcac cccactcgcc gagtgactgg caaaccgtga agagttcgcg
     3421 gcccgcggat gtgagcgcat agtggtgccc acgcccgtcg gacttgggtg ccaactcgac
     3481 gatgccgacg cgttcgagct gctggagccg ctgtgagagc agggtacggg agagcccggg
     3541 cgcgccctcc aagatttcgc tgaaactacc agagcccagg tgcaggttgc ggatgattag
     3601 cggtgtccag cgctcggcga agatctcggc gccacgggcg atggggcagt attggccgta
     3661 cgtcctcacc gcacgagtat cgcgcatccg cgttccgatt tgaagtccag attatggact
     3721 agaccgacgg gtcccgcggt ggtccgattg tgctcatcga aaggagaacc cgatgaacac
     3781 aacgaaaacc acgaaagtcg gccgcgggag cacgaagcca accgtcgcgg ggatcgacgg
     3841 gtaccggggc cggctaatcg gtcccgacca tgccgactac gacgtcgccc gtgccgtgtg
     3901 gaacggttcc atcgaccggc gcccgcgctt gatcgcccga tgcatcgggg ccgccgacgt
     3961 ggtcgccgcg gtacgttttg cccgcgaaca cgatctggag atcgcgctcc ggggcggagg
     4021 ccacaacgtg gcgggcaccg cggtttgcga cgacggcatc gtcatcgacc tctcggccat
     4081 gcgggccgtg cgggtcgatc ccgccgaccg cagggtctgg gtgcagggtg gtgcgctgtg
     4141 gggcgacgtc gatcacgaga cgcaggcgca cgggctggcc accaccggcg gaatcgtgag
     4201 ccacacgggc gtcgcggggc tcaccctcgg cggcggcgtc ggctggctga tgcgcaagca
     4261 cggcctgacg gtcgacaacc ttctcgctgt cgatgtcgtg acggccgacg gcgggcggct
     4321 gcgcgcgtca gagcaagagc accccgacct gttctgggcg ttgcgaggcg gcggcggcaa
     4381 cttcggcgta gtcacctcgt tcgagttctg cctccacccc gtcgggcccg ccgtcctggc
     4441 cg
//
LOCUS       DASPGK010000254         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1352403, whole genome shotgun sequence.
ACCESSION   DASPGK010000254 DASPGK010000000
VERSION     DASPGK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352403"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VEC56_06885"
     CDS             complement(<1..388)
                     /locus_tag="VEC56_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXV13916.1"
                     /translation="MEWIQKEIALAPRPRGFHLVTDEVVRAVPELARFRVGVAHFFIQ
                     HTSASLCINENADATVRTDLERSFNELVPEDAPYYRHTTEGPDDMPAHVKAALIGTSV
                     SVPVTNGRLNLGTWQGLYLCEHRDRGG"
     gene            442..1470
                     /locus_tag="VEC56_06890"
     CDS             442..1470
                     /locus_tag="VEC56_06890"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXV13917.1"
                     /translation="MTPDHRFIAHRRDEWYPIARSDRVGARPLAAMLFGTPLVVFRAG
                     GGVAALVDRCPHRNVPLSMGRVRGANLQCAYHGWEFDRDGQCRAIPGLIGELRAHERR
                     ATSFACQEQQGFVWVWGEAEASPSREPFRFPYIGERGWTTAREELEVRASLHATAENA
                     LDVPHTAFLHGGLFRSETRARREIEVIVRRFDDRVEAEYVGESRPPGIVAKLLAPRGG
                     EVIHFDRFLLPSVAQVEYRLGDSGLCISAALAPVEDYRTRLFIAFSYRLPIPGWMVRP
                     ILRAFAMRIFRQDAAMLARQTETIDRFGEERYTFTEIDILGAEIYDLLRRGRAAPAAA
                     ERRLRIAV"
     gene            1720..2871
                     /gene="carA"
                     /locus_tag="VEC56_06895"
     CDS             1720..2871
                     /gene="carA"
                     /locus_tag="VEC56_06895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXV13918.1"
                     /translation="MSNRARGKMILADGTRFDGYLTGAAARPDWRGPIWGEVVFNTSM
                     TGYQEVITDPSYAGQVVVMTYPQIGNYGVTADDSEKPATAARALVVHDLQEFFSPGPG
                     RVSLESFMMDQNLPGLSGVDTRAVTKHVRDQGAVIAAIGRADASDDELLALARSKKLD
                     RSERLVESVSGTLNAVHPGARGRAAVIDLGVKKSIVDNVRRLGLTTQVFDTTFRADDI
                     LKGGFDFVVLSNGPGDPADIPEVVEQVTKLIGRVPMLGICLGHQIIALALGARTYKLK
                     FGHHGANQPVVDHRTGQVFITSQNHNYAVSDDIADATGVTITYTNANDGTLEGFVDEH
                     RKLECVQFHPEASPGPHDCRVIFDRFHERVQGWSSGASATKGGDAHAAA"
     gene            2858..>4542
                     /gene="carB"
                     /locus_tag="VEC56_06900"
     CDS             2858..>4542
                     /gene="carB"
                     /locus_tag="VEC56_06900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXV13919.1"
                     /translation="MPRLDGIRSVMVIGSGPIVIGQGCEFDYSGTQAIRALREEGLRV
                     VLINSNPATIMTDPEMADATYIEPLTFDACRAIIEKERPDALLPTLGGQTALNLSMEL
                     HDSGVLEEFGVRLIGVDLKAIRRAEDRSQFKLLCDEAGFDTPKSVTVHTLDEAMRSLE
                     SIGLPAIIRPSFTLGGSGSGMARTKDDFARIVKYGLQKSPVREVLIEESVEGWKEFEL
                     EVMRDKDGNHVVVCSIENLDPMGVHTGDSVTVAPVQTLRDPEYQRMRDAAFKIMDLVG
                     IWGGANVQFALEPMTGRMVVIEMNPRVSRSSALASKATGYPIAKVAARLALGYTLAEL
                     PNDIVGTIPAAFEPSLDYVVVKIPRWNFEKFRGVEDVLGSQMKSIGEVMALGRTFNEA
                     LQKALRSLESDWAGLDSVEFAPDDLEKLLAVPNSMRLFAVKAAYAAGWTTERVYQLTK
                     IDPWFLENFRTLVAFEKRLASLAFPPSHAELRQAKRFGFSDRQLATLWRVEEEDVIRA
                     REAMDIHPVVKMVDTCAGEFEARTPYYYVVYDDECDVETDTRRARGTKDKVVIL"
BASE COUNT          783 a         1514 c         1510 g          735 t
ORIGIN      
        1 agccgccgcg gtcacggtgt tcgcacaggt acaaaccctg ccacgtaccc aagttcaaac
       61 gaccgttggt gaccgggacg ctaacactgg ttccgatcaa ggccgccttg acgtgggccg
      121 gcatgtcgtc gggtccttcg gtggtgtggc ggtagtaggg cgcgtcttcg gggacgagct
      181 cgttgaagct tctttccagg tcggtgcgca cggtcgcgtc cgcgttttcg ttgatgcaca
      241 acgacgccga ggtgtgctgg atgaagaaat gcgccacccc gacgcgaaag cgcgcgagct
      301 cggggaccgc gcgcaccacc tcgtcggtga cgagatgaaa gccgcgcggt cgcggcgcga
      361 gcgcgatctc tttctgtatc cactccatgg cggcagggta gcacgcaggg cgccgcgcgc
      421 cgtaatgcta taatcaccgc aatgacgccc gaccatcgtt tcatcgcgca ccgccgcgac
      481 gagtggtatc cgatcgcgcg cagcgaccgc gtgggcgccc gcccgctcgc cgcgatgctc
      541 ttcggcacgc cgctggtcgt gttccgcgcc ggcggcgggg tggccgcgct cgtcgatcgc
      601 tgcccccatc gcaacgtgcc cctttcgatg ggtcgcgtgc ggggtgcaaa tctccagtgc
      661 gcctatcacg gctgggaatt cgatcgcgac gggcagtgtc gtgcgatccc cggattgatc
      721 ggcgagttgc gcgcgcacga gcggcgcgcg acgtcgttcg cctgccagga gcaacaagga
      781 ttcgtgtggg tgtggggcga agccgaagcg tcgccgtcgc gcgagccctt tcgctttccg
      841 tacatcggcg agcgcggctg gaccaccgcg cgcgaagaac tcgaagtgcg cgcgtcgctc
      901 catgcgacgg ccgagaacgc gctcgacgtc ccgcacaccg cgttcctcca cggcggcctg
      961 ttccgaagcg agacgcgcgc gcgccgcgag atcgaggtga tcgtgcgccg cttcgacgac
     1021 cgcgtcgagg ccgaatacgt gggcgagtcg cggccgcccg ggatcgtcgc caagctgctc
     1081 gcgccgcgcg gcggcgaggt gatccacttc gaccgcttcc tcttgccgtc ggtggctcag
     1141 gtcgagtacc gcttgggcga ttccggtctg tgcatttccg ccgcactcgc gccggtcgaa
     1201 gactatcgca cgaggttgtt catcgcgttc tcgtaccgcc tgccgatccc gggctggatg
     1261 gtgcgcccga tcctgcgcgc gttcgcgatg cgcatcttcc gccaggacgc cgccatgctg
     1321 gcgcggcaaa ccgagaccat cgaccgcttc ggagaagagc gctacacgtt caccgagatc
     1381 gacatcctgg gggcggagat atacgatctc ttgcgacgcg gccgcgccgc accggcggcc
     1441 gccgagcgac gcctgcggat cgccgtgtga cagctcgccc cggcgaggtt cgcccttgac
     1501 gccgcggacc cgacccgcta tcctgtgacg caatcgatgt ccttttaacc gggccctgcg
     1561 aggctcagaa gggatcctgc gagtgacgaa cacgacgtcc ccgcgcgcgc gcggggcatc
     1621 cgacagaacg acgcgtcgcc ttcccgcaaa cgccgtggga gggcgttttt ttgtgctcgt
     1681 gcgtggccgt cgcggcggcg acgggagcgg ggtggagcga tgagcaaccg cgcgcgcggc
     1741 aagatgatcc tcgccgacgg aacccgcttc gacgggtacc tgacgggagc ggctgcgcgc
     1801 cccgactggc gcgggcccat ctggggcgag gtcgtgttca atacctccat gacgggctat
     1861 caagaagtca tcaccgaccc ctcctacgcg ggacaggtgg tcgtgatgac gtacccgcag
     1921 atcggcaact acggtgtgac cgccgacgac agcgagaagc cggccaccgc ggcccgggcc
     1981 ctcgtcgtcc acgacctcca ggagttcttc tcccccggac ccgggcgggt gtcgctggag
     2041 tcgttcatga tggatcagaa cctcccgggc ctctccggtg tggacacgcg cgcggtgacc
     2101 aagcacgtgc gcgaccaagg ggccgtgatc gccgccatcg gccgcgccga cgcctccgac
     2161 gacgagctac tcgcactcgc acgctcgaag aagctcgatc gctccgagcg cctggtcgag
     2221 agtgtctcgg gcacgctgaa cgcggtccac cccggcgcgc gcgggcgcgc ggccgtgatc
     2281 gacctcggcg tcaagaagag catcgtcgac aacgtgcgac gcctgggact gaccacgcag
     2341 gtcttcgaca ccacgtttcg cgcggacgat atcctgaagg gcggcttcga cttcgtggtg
     2401 ctctccaacg gccccggcga ccccgccgac atcccggagg tggtcgagca ggtgacaaag
     2461 ctcatcggcc gcgtcccgat gctcgggatc tgcctcggcc accagatcat cgccttggca
     2521 ctgggcgcgc ggacgtacaa gctgaaattc ggacaccacg gcgcgaacca gccggtcgtc
     2581 gaccatcgaa ccggccaggt gttcatcacc agccagaacc acaactatgc cgtcagcgac
     2641 gacatcgcgg acgcgacggg agtcaccatc acctacacca acgccaacga cggaaccttg
     2701 gaaggcttcg tcgacgagca ccgcaagctc gagtgcgtgc agttccaccc cgaggcttca
     2761 ccggggccgc acgattgccg cgtgatcttc gatcgcttcc acgagcgtgt gcagggatgg
     2821 tccagcggcg cctcggcgac gaaggggggc gatgcgcatg ccgcggcttg acggaattcg
     2881 atccgtgatg gtgatcggca gcggccccat cgtcatcggt cagggctgcg agttcgacta
     2941 ctcggggaca caggcgatcc gtgccttgcg cgaagaaggc ttgcgcgtcg tgctcatcaa
     3001 cagcaacccc gccaccatca tgaccgatcc ggagatggcg gacgcgacct acatcgagcc
     3061 gctgaccttc gacgcgtgcc gcgccatcat cgagaaagag cgccccgacg cgctcttgcc
     3121 gacactcggc gggcagaccg cgctcaacct ctcgatggag ctgcacgact ccggggtgct
     3181 ggaggaattc ggcgtgcgcc tgatcggcgt cgacttgaag gccatccggc gcgcggagga
     3241 ccgcagccag ttcaagctcc tgtgcgacga ggccggtttc gacacgccga agagcgtcac
     3301 cgtgcacacc ctcgacgaag cgatgcgctc gctggagtcg atcgggctgc ccgcgatcat
     3361 ccgcccctcg ttcacgttgg gcggaagcgg cagcggcatg gcgcgcacga aggacgactt
     3421 cgcgcgcatc gtgaagtacg gattgcagaa gtcgccggtg cgcgaagtgc tgatcgaaga
     3481 atccgtcgaa ggctggaagg aattcgagct cgaggtcatg cgcgacaagg acggcaacca
     3541 cgtggtggtg tgctcgatcg agaatctcga cccgatggga gtgcacaccg gcgacagcgt
     3601 gaccgtcgcc ccggtgcaga cgctgcgcga ccccgaatac cagcgcatgc gcgacgccgc
     3661 cttcaagatc atggacctgg tcggtatttg gggcggggcc aacgtacagt tcgcgctcga
     3721 accgatgacg ggacgcatgg tcgtgatcga gatgaacccg cgcgtgtcgc gctcgtcggc
     3781 gctggccagc aaggccaccg gctacccgat cgccaaggtg gccgcacggc tcgcactcgg
     3841 ctacacgctg gcagagttgc ccaacgacat cgtgggcacc atcccggccg ccttcgagcc
     3901 ctcgctcgac tacgtcgtgg tcaagatccc gcgctggaac ttcgagaagt tccgcggcgt
     3961 cgaggacgtg ctgggctcgc agatgaaatc gatcggcgag gtgatggcgc tcgggcgcac
     4021 cttcaacgaa gccctgcaga aggcattgcg ctcgctggaa tcggattggg ccggactgga
     4081 ctcggtcgag ttcgcgcccg acgatctcga gaaactcctc gcggtgccca actcgatgcg
     4141 gctgttcgcg gtcaaggcgg cgtacgcggc ggggtggacg acggagagag tctaccagct
     4201 caccaaaatc gatccttggt tcttggagaa tttccggacc ttagttgcct tcgaaaagag
     4261 gctcgcttcg ctcgcttttc ctccgtcgca cgctgaattg cggcaagcga agcgattcgg
     4321 gttcagcgac cgccagctcg ccaccctgtg gcgcgtggaa gaagaagacg tgattcgcgc
     4381 gcgcgaggcg atggacatcc accccgtggt caagatggtc gacacctgcg cgggcgagtt
     4441 cgaggcgcgc acgccctact actacgtcgt ctacgacgac gagtgcgacg tcgagaccga
     4501 cacgcgacgc gcgcgcggta ccaaggacaa ggtcgtgatc ct
//
LOCUS       DASPGK010000255        10565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_533980, whole genome shotgun sequence.
ACCESSION   DASPGK010000255 DASPGK010000000
VERSION     DASPGK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533980"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1301)
                     /locus_tag="VEC56_06905"
     CDS             complement(156..1301)
                     /locus_tag="VEC56_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV13920.1"
                     /translation="MDFSWSEEQTNLRESIATFAREELNQGLIERDQKGEFNREGWDK
                     CARMGVHGLPVPTAYGGLGMDALTTVGVLESLGYGCEDNGLVFSINAHMWTLETPIMG
                     FGTEEQKKRLLPKLTSGEWIGANAMSEPGSGSDAYSIATTAKKKGDVYLLNGSKVFCS
                     NGPIADVYLAFANVDPALGANGVTGFLVERTSPGLSLGKHTEKMGLRTSPMCEVFFED
                     CEVPEANRLGREGAGKMLFADSMSWERGCILASAVGTMRRLLETSIAYATSREQFGQP
                     IGKFQMVQSKIVDMKLRLDTARAFLYQSAYQKSRGKSVYLEAAMAKLYISESWIECAR
                     HALQIHGGYGYTVEYKIERELRDALASTLFSGTSEIQRTIIASLMGL"
     gene            complement(1304..2881)
                     /locus_tag="VEC56_06910"
     CDS             complement(1304..2881)
                     /locus_tag="VEC56_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXV13921.1"
                     /translation="MTLQRYLDETVARFPERAAVREPGGASVTYRELGELSDRLRDRL
                     ASMGVAPGDRVGIYLRKSIDTVAAIFGVLKTGAAYVPVDPSAPPSRNGYIFANCSVKV
                     AITERRFEPGLRKHLSEEGAADTPLFVIDEAAGGAGLRAALDAADAATRAPRVATVHT
                     GPDALAYILYTSGSTGKPKGVILTQRNATSFVDWCSETFAPAPEDVFSSHAPFHFDLS
                     ILDIYTPLKHGASLALVPEDIGKDPQKLARLIHDARITVWYSTPSVLSLLVQYGKLED
                     YDLSHIRTILFAGEVFPIVHLKALHALLPAPRYFNLYGPTETNVCTFFELPPAIPSER
                     VDPFPIGMVCSHLEAVVVDDAGDEVGRGERGELCIRGASVTPGYWNLPEQTAKAYLTT
                     GVGARDAAKGPWYKTGDVVTEETGGVYVYAGRKDRMVKKRGYRVELGEIESCLYRHPD
                     VREAAVVAIPDDELGLKVRAHLATRDGGRLSVIALKGFCAQHLPVYMIPDVFSFHAEL
                     PKTSTEKTDYQALMRME"
     gene            complement(2886..3137)
                     /locus_tag="VEC56_06915"
     CDS             complement(2886..3137)
                     /locus_tag="VEC56_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXV13922.1"
                     /translation="MDRQAIVDTVKHFILEQFLPGEDPDELATDTALMTTGILDSLAT
                     LKLVTFLEQEFDITVEAHEADAANLNTLDLIADLVHGKL"
     gene            complement(3154..5661)
                     /locus_tag="VEC56_06920"
     CDS             complement(3154..5661)
                     /locus_tag="VEC56_06920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXV13923.1"
                     /translation="MTSPVQTSKYKLGPLARFGMVAAQLALLTLVVRTFDIESGAFFR
                     VMVIASIGFVVHHLAPSRLRLPLFVLISVAGIVTVLGPANAAQIVGLGLLLIGIAHLP
                     VRFSARVALILALGGLLAAARVGVVRAPLSTAIWPIFGAMFMFRMIVYLYDLKNRAAP
                     FGPWRALAYFFMLPNVCFPLFPVVDYKSFCRTYYDDEDVSIYQTGVEWIFNGIVQLLI
                     YRWVYQNLLVGPDAVANASQAALFLFTAYAMYLKISGSFHMIVGILHVFGFNLGPTNN
                     KYFLSQSFTDYWRRINIYWKDFLQKVFFNPVYFRLNKKMGATSALVFATLVAFFVTWA
                     LHSYQWFWIRGNFPVIWQDIAFWSIMGLLVLGNMLYENKYGRQRSLKKAKVSAASEVT
                     LALRTVGTFVVICVSWVIWSSESFGEVTMVLGKLARPDLRAVLWIVLGLGALGAIAIA
                     VARHERGKAGGALARASRKGPAPILGRAYQVGAACVAILFIAYAPLVIDIDPAISNTV
                     DNLKTSRLNARDAQMLDRGYYEDLTNVVRFNTTLGDLYNEKPPNWERCWAMHPIDGYP
                     DYEFMPNKNVWFKGTMMSTNQWGLRDRDCEKSKPAGTYRIAMLGASHPMGTGVEDDQV
                     MDNVAEDRLTRESPTGEHERYEILNFSVGGYGPIQMLADMDRRVFDFEFDALLWTGID
                     DIYWTAKDLVDAANHGFAIPYPHVMDVMREAGIGRDTPFSDGLNRMKPHSEALLSWIY
                     AQVVERCAEHGVVPMAAFLPHMSDISDEHELLARKARQMELARAAGMVVLDLTKAYRG
                     VDPRTMWIAPWDSHPNADGHRMLGDELYEALKVQLAL"
     gene            complement(5667..6704)
                     /locus_tag="VEC56_06925"
     CDS             complement(5667..6704)
                     /locus_tag="VEC56_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV13924.1"
                     /translation="MRYLVTGGSGFLGINLIRYLIERGHAVVSLDLLPFDYEDCRDRV
                     EAVVGDIRNRAAVDRAMKGVEIVVHCAAALPLYSKADILSTDIDGTRNVLQSALEAKV
                     QRVIHVSSTAVYGVPDHHPLYETDPLVGVGPYGEAKIKAEEVCVEYRKQGMCVPILRP
                     KSFVGPERLGVFALLFDWARDGRNFPLPGRGDNLYQLMDVADLCDAIYLCATLDRDTV
                     NDVFNVGAKEFDTVKNDFQAVLDRAGKGRRIVPLPEAPMKLALAILRALRLSPVYKWA
                     YGTVSKESFVSIEKAERVLGFKPRYSNQAALLRNYEWYLANLHRFRGSSGVTHRVPWS
                     QGILSLAKRFF"
     gene            complement(6771..7262)
                     /gene="greA"
                     /locus_tag="VEC56_06930"
     CDS             complement(6771..7262)
                     /gene="greA"
                     /locus_tag="VEC56_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXV13925.1"
                     /translation="MAQYFLTEKGYLKLKEEIDRLDRYLKNDIAKEIATAAAHGDLKE
                     NGEYHAAKEKQAHTAAKLRQLQERFSGANVVRKEELLPVESVTFGKKIKIRDTKTGNE
                     RVCTILGEGETDPENGVIAYNSPLASALIGHSKGETVDVQLPVGMKTFQILEVEFAEG
                     YQD"
     gene            7481..8059
                     /locus_tag="VEC56_06935"
     CDS             7481..8059
                     /locus_tag="VEC56_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV13926.1"
                     /translation="MKRFSVVLAAVAFCTLMAADASAQANFALRGIGLKAGVVNPEDL
                     DTTLGLGLIFDLGTIHPDWAFESYAGYWSQTEEDFGGEFGVRDFSFGAKTKYMFKTSN
                     PAVQPFAGGGLGLHVLSAHAEIDPISVGGTVIFPGSSVEETELRVGLDLGGGLRYDAG
                     KQFAFLGEGWFSVVSDASHFSLMVGAVYMFGR"
     gene            complement(8293..9027)
                     /locus_tag="VEC56_06940"
     CDS             complement(8293..9027)
                     /locus_tag="VEC56_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXV13927.1"
                     /translation="MYAPSDTATRSTGPDRSAAIIDVVVYLVAVVVFWGVEEWLRSIG
                     RFPLPGLLDGAPSLVASFFVVVALMKRRGQRWSDFGLVRPRRWWLVPAWGLVVMVVHV
                     ASQMTIVPLLSSLLHVPPPDFSKYDPLRGNLKLFLFVTPGAMITGGFIEEFVYRGMMF
                     DRLARIVGGTGRRTVLAAALLCGIPFGLAHFQWGVGGVLVTAVMGSMLGLMYLATRRN
                     LWPLVFGHAALDFLLLLQVYLGVLKP"
     gene            complement(9074..9150)
                     /locus_tag="VEC56_06945"
     tRNA            complement(9074..9150)
                     /locus_tag="VEC56_06945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9114..9116),aa:Arg,seq:tct)
     gene            complement(9204..10202)
                     /locus_tag="VEC56_06950"
     CDS             complement(9204..10202)
                     /locus_tag="VEC56_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV13928.1"
                     /translation="MRESLLRYLVCPRDHAELSLSADTRDGDRIATGTLRCSACQAEY
                     PIRHYVPRFVADDQYVASFSHEWTRFARTQLDSVNGTDISLQYFRNSVKRAPADYAGS
                     LVLDAGCGTGRMLELFADVEATVIGIDLSYAVDSAVDNVGKHPHLHVVQASIYDLPFR
                     PATFDFAYSIGVLHHTPDPERAFRAVQDVVRPGGDVGIRVLSKRMVGGVNLDWIFPLF
                     LYRHVTRRARPDHLLAFLKGYVPVACSLRRIPLVGQFFRWLLPVADYHGVHPLDDEQL
                     RQWNILDTFDRFSPRYYFRYSDADLRAWFPSDRFDHFERGTFPVSARGRRASASRT"
     gene            complement(10224..>10565)
                     /locus_tag="VEC56_06955"
     CDS             complement(10224..>10565)
                     /locus_tag="VEC56_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HXV13929.1"
                     /translation="YLPFETTSTIFVGGCIRWVMDRTSERRGHNEAQRTRVVNVGILA
                     ASGMIAGEALVGLVKSTMAWMEKPFPQVLANPSYLVGLLVLGLIAFVLIRVPLRNAGD
                     PNEPAPPAAMV"
BASE COUNT         1972 a         3362 c         3416 g         1815 t
ORIGIN      
        1 tcttccgccc catgacgaga cagtcgacgc tgcggtagaa ctcggcgaac ccgtagtcgt
       61 cgtcggtgaa gagccagtcg atggcgtcgt tggggccggc gatgaagccg tccacgctgc
      121 aggcgacgaa gtagcacacg tctctcatcg tgtcctcaca gtcccatcag cgacgcgatg
      181 atggtgcgct ggatctccga ggtcccggag aacagcgtgc ttgcgagcgc gtcgcgcagc
      241 tcgcgctcga tcttgtattc caccgtgtag ccgtaccctc cgtggatctg cagcgcatgg
      301 cgcgcgcact cgatccacga ctcggagatg tatagcttgg ccatggcggc ttccaggtac
      361 acgctcttgc cgcgcgattt ctgataggcc gattgataga gaaaggcgcg cgcggtatcg
      421 agccgcagct tcatgtcgac gatcttcgac tggaccattt ggaacttgcc gatgggctgg
      481 ccgaactgct cgcgcgaggt ggcgtacgcg atcgacgtct ccaagagccg ccgcatcgtg
      541 ccgaccgcgg aggcgagaat acacccgcgc tcccacgaca tcgagtcggc gaagagcatc
      601 ttgccggcac cctcgcggcc caggcggttg gcctcgggaa cctcgcagtc ctcgaagaag
      661 acttcgcaca tgggcgacgt gcgcaggccc atcttctcgg tgtgtttgcc gagcgagagc
      721 ccgggcgagg ttcgctccac caggaagccg gtgacgccgt tggcacccag cgcggggtcg
      781 acgttggcga aggcgaggta cacgtcggcg atgggtccgt tggagcagaa caccttgctc
      841 ccgttcaaga gatagacgtc gcccttcttc ttcgcggtgg tcgcgatgct gtaggcgtcg
      901 gagcccgagc ccggctcgga catggcgttg gcgccgatcc actccccgct cgtgagcttg
      961 ggaaggagtc tcttcttctg ctcctcggtg ccgaagccca tgatgggcgt ctcgagcgtc
     1021 cacatgtgcg cgttgatcga gaacacgagg ccgttgtctt cgcacccgta ccccagactc
     1081 tcgagcacgc ccaccgtggt cagcgcgtcc atgcccagcc cgccgtacgc ggttggcacc
     1141 ggtagtccgt gcacacccat gcgcgcgcac ttgtcccagc cctcgcgatt gaactcgccc
     1201 ttttgatcgc gctcgatgag gccctggttc aactcctcgc gcgcaaacgt cgcgatgctc
     1261 tcgcgcaggt tcgtctgttc ttccgaccac gagaagtcca tcgctactcc atcctcatga
     1321 gagcttggta gtcggtcttt tccgtcgacg tcttgggcag ttcggcgtgg aagctgaaca
     1381 cgtcggggat catgtagacc gggaggtgct gcgcgcagaa tcccttgagc gcgatgaccg
     1441 aaagacggcc gccgtcgcgg gtggcgaggt gggcgcggac cttcaagccc aattcgtcgt
     1501 cggggatcgc gaccaccgcg gcctcgcgca cgtcgggatg gcggtacaag cacgattcga
     1561 tttcccccag ctcgacgcgg tagccgcgct tcttcaccat gcggtccttg cggcccgcgt
     1621 agacgtacac gccgccggtt tcctcggtga cgacgtcgcc cgtcttgtac cagggaccct
     1681 tcgctgcatc gcgcgcgccc acgcccgtcg tgaggtaggc cttcgcggtt tgttcgggga
     1741 ggttccaata gcccggcgtc accgacgctc cgcggatgca cagctcgccg cgctcgcccc
     1801 gtcccacttc gtcgcccgca tcgtcgacca ccaccgcctc caggtgcgaa cacaccatac
     1861 cgatcggaaa cgggtcgacg cgctcggaag gtatcgcggg cgggagttcg aagaaggtgc
     1921 acacgttggt ttcggtgggt ccgtacaggt tgaaatagcg cggcgcgggc aggagcgcgt
     1981 gcagcgcctt caggtgcacg atgggaaaca cctcgcccgc gaacaggatg gtgcgaatgt
     2041 gcgagaggtc gtagtcctcg agcttcccgt actggaccag caggctcaag accgacgggg
     2101 tcgagtacca caccgtgatg cgcgcgtcgt ggatcaagcg cgcgagcttc tgcgggtcct
     2161 tgccgatgtc ttcgggtacc aacgcgagag acgcaccatg tttgagcggc gtgtagatat
     2221 cgaggatcga caggtcgaag tggaagggcg cgtgcgagga gaacacgtct tcgggcgccg
     2281 gcgcgaacgt ctcgctgcac caatcgacga agctggtcgc gttgcgctgc gtgagtatta
     2341 cacccttggg cttcccggtc gagcccgacg tgtagagaat gtaggccagc gcgtcggggc
     2401 cggtgtggac ggtcgcgaca cgcggcgcgc gcgtggcggc gtcggccgcg tccagggcgg
     2461 ctcgcaagcc cgcaccgcct gcggcttcgt cgatcacgaa caagggcgtg tccgcggcgc
     2521 cctcttcgga gaggtgcttg cgcaagcccg gctcgaagcg cctctccgtg atcgccacct
     2581 tcaccgagca gttcgcgaag atgtagccgt tgcgcgacgg cggcgcggac gggtcgaccg
     2641 gcacgtaggc ggcgccggtc ttgagcacgc cgaagatcgc ggcgaccgtg tctatggact
     2701 tacgcaggta gataccgacg cggtcgccgg gggcgacgcc catcgacgcc aagcggtcgc
     2761 gcagccggtc ggaaagctcg cccaactccc ggtaggtgac cgacgccccg ccgggctcgc
     2821 gcacggcggc acgctccgga aagcgcgcca ccgtttcgtc taaatagcgt tgcagcgtca
     2881 tgcggttaca acttgccgtg caccaggtcc gcgatgagat ccagcgtgtt cagattggcg
     2941 gcgtcggcct cgtgcgcctc gaccgtgatg tcgaactcct gctccaggaa cgtcaccagc
     3001 ttgagcgttg ccagcgaatc gagaattccg gttgtcatga gcgcggtatc tgtcgcgagc
     3061 tcgtccggat cctcgccggg caagaactgc tcgaggatga agtgcttcac cgtatcgacg
     3121 atcgcctgtc ggtccatggt gcgtcctttc gcgtcacaat gccaattgta ccttgagtgc
     3181 ctcgtacagc tcgtcgccca gcatgcgatg gccgtccgcg ttggggtgcg agtcccacgg
     3241 cgcgatccac atggtgcgag gatcgacgcc ccggtaggcc ttcgtcaagt cgagcacgac
     3301 catcccggct gcgcgcgcga gttccatctg gcgggccttg cgcgcgagca gctcgtgctc
     3361 gtccgagata tcgctcatgt gcggaaggaa ggccgccatc ggcaccacgc cgtgctcggc
     3421 gcagcgctcc acgacctgcg cgtagatcca cgacaacaac gcctcgctgt ggggcttcat
     3481 tcgattgagg ccgtcggaga acggcgtgtc gcgcccgatg ccggcttcgc gcatgacgtc
     3541 catgacgtgc gggtatggga tcgcgaagcc gtggttggcc gcgtcgacca ggtccttcgc
     3601 ggtccagtag atgtcgtcga tgccggtcca caagagcgcg tcgaactcga agtcgaacac
     3661 gcggcggtcc atgtcggcga gcatctggat ggggccgtat ccgcccaccg agaaattgag
     3721 gatctcgtag cgctcgtgct cgccagtcgg actctcgcgc gtcagacgat cctccgcgac
     3781 gttgtccatg acctggtcgt cttccacgcc ggtacccatc gggtgcgacg cccccagcat
     3841 ggcgatgcgg taggtgccgg ccggcttgct cttctcgcag tcgcgatcgc gcagacccca
     3901 ttggttggtg ctcatcatgg tgcccttgaa ccacacgttc ttgttgggca tgaactcgta
     3961 gtcggggtaa ccgtcgatgg ggtgcatggc ccagcagcgc tcccagttgg gcggtttctc
     4021 gttgtagagg tcgccgagcg tcgtattgaa gcgcaccacg ttggtcaggt cctcgtagta
     4081 gccgcggtcg agcatctgcg cgtcgcgcgc gttcagacgc gacgtcttca ggttgtcgac
     4141 cgtattactg atggccgggt cgatgtcgat cacaagcggt gcgtaggcga tgaacagaat
     4201 cgccacgcag gccgcaccca cctgataggc acgtcccaag atgggcgcgg ggcccttccg
     4261 actggctcgc gcgagcgccc ctcccgcttt tccgcgctcg tggcgcgcga cggcgatcgc
     4321 gatggccccg agcgctccca ggccgagaac gatccagagc accgcgcgca gatcgggacg
     4381 cgcgagcttg cccagcacca tggttacttc gccgaacgat tccgacgacc agatgaccca
     4441 actcacgcag atcaccacga aggtcccgac ggtccgcaac gccagcgtga cctcgctcgc
     4501 ggccgagacc ttggccttct tcagacttcg ttggcgcccg tacttgttct cgtagagcat
     4561 attgcccagc acgagcaacc ccatgatcga ccagaacgcg atgtcctgcc agatcaccgg
     4621 gaagttaccg cggatccaga accactggta cgagtgcagc gcccaagtga cgaagaacgc
     4681 gaccagcgta gcgaacacga gcgcgctcgt cgcacccatc ttcttgttca aacgaaaata
     4741 gaccgggttg aagaacacct tctggagaaa gtctttccag tagatattga tgcgccgcca
     4801 gtagtcggtg aagctttggg agaggaagta cttgttgttg gtcggcccca ggttgaaccc
     4861 gaatacgtgc aggatgccga cgatcatgtg gaagctgccc gagatcttga ggtacatcgc
     4921 gtacgccgtg aagaggaaca acgccgcctg cgacgcgttc gcgaccgcgt ccggaccgac
     4981 caggagattc tggtacaccc accgataaat caggagctgg acgatgccgt tgaagatcca
     5041 ctcgactccg gtttgataga tgctcacgtc ttcgtcgtcg tagtaggtac gacaaaacga
     5101 cttgtagtcg accaccggga acagcgggaa gcacacgttg ggcagcatga agaagtaggc
     5161 cagcgcgcgc caggggccga agggcgccgc gcggttcttc aggtcgtaca ggtagacgat
     5221 catccggaac atgaacatgg cgccgaagat gggccagatc gcggtcgaga gcggcgcacg
     5281 caccacaccg acgcgcgcgg ccgcgagcag cccccccagc gccaggatca gcgcgacgcg
     5341 cgccgaaaag cgcaccggaa ggtgcgcgat cccgatcaac aacagaccca ggccgacgat
     5401 ctgggccgcg ttggccggtc ccaggacggt cacgataccg gcgaccgaga tgagcacgaa
     5461 caacggcagc cgcaggcgcg acggtgccag gtgatggacg acgaaaccga tgctcgcgat
     5521 caccatcacg cggaagaacg cgccgctttc gatatcgaag gtgcggacga ccagggtcag
     5581 caacgcgagc tgtgccgcga ccatgccgaa gcgcgcgagc ggccccagtt tgtatttact
     5641 cgtttggacc ggcgacgtca tggcgtctag aagaaccgct tggcgagtga caggatcccc
     5701 tggctccacg gcacgcggtg cgtgacaccc gacgaaccgc ggaaacggtg gaggttggcc
     5761 aggtaccact cgtagttgcg cagcaacgcc gcctggttcg agtagcgcgg cttgaagccg
     5821 aggacgcgtt cggccttctc gatcgacacg aaggactcct tcgacaccgt gccgtacgcc
     5881 cacttgtaaa ccggcgagag ccgcagcgcg cgcagtatcg cgagcgcgag cttcatgggc
     5941 gcttccggta gcgggacgat gcgcctgccc ttccccgcgc ggtcgagcac ggcctgaaag
     6001 tcgttcttca ccgtgtcgaa ctccttggca cccacgttga acacgtcgtt gaccgtgtcg
     6061 cgatccagcg tcgcgcacaa gtagatcgcg tcgcacaggt cggcgacgtc catgagctgg
     6121 tagaggttat cgccgcgccc gggcagcggg aagttgcggc cgtcgcgcgc ccagtcgaag
     6181 agaagcgcga atacgcccag gcgctcgggc cccacgaacg acttgggccg caagatggga
     6241 acgcacattc cctgcttgcg gtactcgacg cagacttcct cggccttgat cttcgcctcg
     6301 ccgtacgggc ccacacccac cagcggatcc gtctcgtaca gcggatgatg gtcgggcacg
     6361 ccgtacaccg cggtcgacga gacgtggatc acgcgctgga ccttggcctc gagcgccgac
     6421 tgcaagacgt tgcgcgtccc gtcgatgtcg gtcgagagaa tatcggcctt ggagtatagc
     6481 gggagcgcgg cggcgcagtg cacgacgatc tcgacgccct tcatcgcacg gtcgaccgcg
     6541 gcgcggtttc tgatatctcc gaccaccgcc tctacgcgat cacggcagtc ctcatagtcg
     6601 aacggcagga ggtcgagcga aaccaccgcg tgcccgcgct cgattagata tcgaatgagg
     6661 ttgatgccga ggaatccgga cccgccggtg acgaggtagc gcatcggaac tgaaactccc
     6721 ggttgttgga tggtacgacg aagtggcgtg gtgaagcgcg agcgtcggcg ctagtcctgg
     6781 tacccctcgg cgaattcgac ttccaggatc tggaacgtct tcattcccac gggaagctgc
     6841 acgtccaccg tttcgccctt gctgtgcccg atcaacgcgc tcgcaagcgg cgagttatag
     6901 gcgatgacgc cgttctcggg gtcggtttcg ccttcgccga ggatggtgca cacgcgctcg
     6961 ttgccggtct tggtgtcgcg aatcttgatc ttcttaccga aggtcaccga ctccaccggc
     7021 aagagctcct ccttgcgcac gacgttggcg ccgctgaagc gctcctggag ctggcgcagc
     7081 ttggcggcgg tgtgggcctg tttctccttc gcggcgtggt actcgccgtt ctccttcaag
     7141 tcgccgtgcg cggccgccgt cgcgatctcc ttggcgatat cgttcttcaa gtagcgatcg
     7201 aggcgatcga tctcttcctt gagcttgaga tagcccttct cggtcaagaa atactgcgcc
     7261 ataccaaccc ttctgatcgg acaatcgagc ctgttgtgtc gagcggaccc gctctgcggt
     7321 ggtcgcgact ctgcttgcct gggggagcgg taactctcgt ggccgtatga gcttatcacg
     7381 aacgccaggg aagcacaaca agggccgccc tggttcgcgt cttgcaccct ctccttggcg
     7441 agaactcgac aaggtggtcc gttcaattgg aggctatgca atgaaacgat ttagcgtggt
     7501 cctggcggcc gtcgcgtttt gcacgctgat ggccgcggac gcgtcggcgc aggcaaactt
     7561 tgccctgaga ggcatcggac tcaaggcggg cgtggtcaat cccgaggacc tcgacaccac
     7621 cctgggactc ggtctcatat tcgacctcgg gaccattcac cccgactggg cgttcgaaag
     7681 ctacgccggc tattggtcgc agaccgagga agacttcggc ggcgagttcg gcgtgcgcga
     7741 cttctcgttc ggggccaaga ccaagtacat gttcaagaca tcgaatccgg ccgtgcagcc
     7801 gttcgccggc ggcggcctgg gattgcatgt gctcagcgct cacgccgaaa tcgatccgat
     7861 ctcggtcggg ggcacggtca tcttcccggg ctcgtcggtg gaagaaaccg agctcagggt
     7921 cggcctggac ttgggcggcg ggctccgcta cgacgcaggc aagcagttcg cgttcctcgg
     7981 cgaaggctgg ttcagcgtcg tcagcgacgc gagccacttc tcgctgatgg tgggcgccgt
     8041 atacatgttc ggtcgttagg gtagacacag aatcgctgca acgaaacgcc ccgcgaggac
     8101 cgctcgcggg gcgtttttct ttacgcggca gttgagcagt aaaccgtcag cgcaccatca
     8161 cgaagcgcga cgacaccgaa cgtcccgatt gcgtgagccg caggaagtag acgccggacg
     8221 ggtaatcctt agtgtgtccg atgtggaggc ggtgagcccc ggcgccgaag cctccgaccg
     8281 cgaggccaca cactacggtt tcagcacccc caggtagacc tggagcagca acaggaaatc
     8341 cagcgccgcg tggccgaaga cgagcggcca caggttgcgc cgtgtggcga gatacatcag
     8401 tcccagcatg gagcccatga ccgccgtcac cagcaccccg ccaacgcccc actgaaagtg
     8461 ggcgagtcca aagggaatgc cgcacagcag agccgcggcg agcacggttc gccgaccggt
     8521 gccgccgacg atacgcgcga ggcgatcgaa catcatgccg cggtacacga actcttcgat
     8581 gaaaccgccc gtgatcatgg cgccgggcgt gacgaacaag aacagtttca ggttgccgcg
     8641 aagcgggtcg tacttggaaa agtcgggcgg ggggacgtgc agtaaactcg aaaggagcgg
     8701 aacgatcgtc atctgcgacg cgacgtggac gaccatgacg accagccccc acgccggcac
     8761 gagccaccag cgtcgcggtc gcacgagacc gaaatccgac cacctctgtc cgcgccgctt
     8821 catcaaggcg acgaccacga agaacgacgc gaccaggctg ggcgcaccgt cgagcaaacc
     8881 aggaagcggg aagcgcccga tggatcgcag ccactcttcg acgccccaaa agacgaccac
     8941 tgcaacgagg taaacgacga cgtcgatgat cgcggcggag cggtcggggc cggtcgatcg
     9001 cgtggcggta tcagagggag cgtacacctt gcggagaggt acggtggcgg ccgtctcgaa
     9061 gttgccaaag aagtggcgac cccgccagga ttcgaacctg ggaccagcag cttagaaggc
     9121 tgctgctcta tccacctgag ctacggggtc gcgatgccca tcatagcatt gcccgcacgg
     9181 aagcgaaacg gagtcgacgc gggtcacgtg cgcgaggcgg aggccctgcg cccgcgggcg
     9241 gaaacgggaa atgtcccgcg ctcgaagtgg tcgaagcgat ccgaagggaa ccacgcgcgc
     9301 aggtcggcgt cggagtagcg gaagtagtag cgaggggaaa agcgatcgaa ggtgtcgagt
     9361 atgttccact gccgcagttg ttcgtcgtcg agcggatgga ccccgtggta gtcggccacg
     9421 gggagcagcc aacgaaaaaa ttggcccacc agcgggatcc gtcgcagcga gcacgccacc
     9481 ggcacatagc ccttgagaaa cgcgagcaga tggtccgggc gcgcgcggcg ggtgacgtgc
     9541 cggtacagga acagcggaaa gatccagtcg agattgacgc cgccgaccat gcgcttgctg
     9601 agcacacgaa tccccacgtc gccgcccgga cgcacgacat cctgcacggc acggaacgcg
     9661 cgctcggggt ccggcgtgtg gtgcagcacg ccgatgctgt aggcgaaatc gaacgtcgcg
     9721 ggacgaaacg gcagatcgta gatgctggcc tgcacgacgt gcaggtgggg gtgcttcccc
     9781 acgttgtcca ccgcggagtc gaccgcgtaa ctcaagtcga tcccgatcac ggtggcctcg
     9841 acgtccgcga acagctccag catgcggccg gtgccgcacc cggcatccag caccagtgac
     9901 ccggcatagt cggccggcgc gcgctttaca gaattgcgaa agtactgcag cgaaatatcc
     9961 gtgccgttga ccgagtcgag ctgggtgcgc gcaaagcgcg tccactcgtg gctgaagctg
    10021 gccacgtact ggtcgtcggc cacgaatcgc ggcacgtagt gacggatcgg atactcggct
    10081 tggcaggcgg agcaacgcag cgtccccgtg gcaatgcggt cgccatcgcg ggtgtccgcg
    10141 ctcagcgaga gctcagcgtg gtcccgcgga cacaccagat accgcagcag tgattcgcgc
    10201 acgaacaact cctgaaagcg gagctacacc atcgcggccg gcggcgccgg ttcgttggga
    10261 tcgcccgcgt tgcggagcgg aacgcgaatc aagacgaacg cgatcaaccc cagtacgagg
    10321 agcccgacca ggtacgacgg gttcgcgagc acctgcggga agggtttctc catccacgcc
    10381 atggtcgact tgaccagtcc caccagcgct tcgcccgcga tcatacccga cgcggccaag
    10441 atgccgacgt tcaccacgcg agttctttgc gcctcgttgt gaccgcgacg ctccgacgtg
    10501 cgatccatca cccagcggat gcagccgccg acaaaaatcg tcgacgtggt ttcgaacggc
    10561 aggta
//
LOCUS       DASPGK010000256         7288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_65657, whole genome shotgun sequence.
ACCESSION   DASPGK010000256 DASPGK010000000
VERSION     DASPGK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65657"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VEC56_06960"
     CDS             complement(<1..1095)
                     /locus_tag="VEC56_06960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HXV13930.1"
                     /translation="MPVAHAQVNEFVPGQVIVKLAPASRTVDAASLRTSVNATVKARF
                     HSIGAELWEIAGIGVPQAIARLQGDPRIVFVEPNYIVHAVDVFPNDEHFADQWNLHNT
                     GQTGGTPDADVDAPEAWTYVTGGDVLVGIIDSGVDWQHVDLAPNIFVNTREIADNRID
                     DDGNGYIDDVRGWDFVNEDNDPDDDNGHGTHVAGIVAAVGNNAIGVAGVSWSARILPL
                     KFLSAVGSGTTSDAIRAVEYATAMGARLTNNSWGGIGFSLALRSAIDEAAEAGALFVA
                     GAGNAGLNNDVTPFYPASFDLDNVISVASTDHDDERSFFSNYGLTSVDLGAPGSDVIS
                     TFPDDRYAIASGTSMAAPHVSGALCLLWTVA"
     gene            complement(1291..2709)
                     /gene="boxB"
                     /locus_tag="VEC56_06965"
     CDS             complement(1291..2709)
                     /gene="boxB"
                     /locus_tag="VEC56_06965"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HXV13931.1"
                     /translation="MDYKELIPNNVNLKENPKLLRALERWHPRFLEWWRDQGPVAHQE
                     REIYLRTAVSVDSSGWAVYDYVRMPEYRWGIFLAPVKSERSIHFGDYIGDGCWEDVPG
                     EHRNELKRLIVTQGDTEPGSVEQQRRLALSAPSLYDMRNLFQVNVEEARHLWAMVYLL
                     HSYFGKDGRDEAEEMLERRSGDPDTPRLLSTFNDPIDDWLDFFCFATYTDRDGKYQLG
                     SLAESAFTPLSRTTRFMLTEEAHHLFVGETGLARTIRRTAQLMKENESEDVRPYGGIP
                     LDIVQKYINYWYSVSLDLFGNEDSSNAANYFAHGLKGRFNEANRKLYPDASAREGNYS
                     YTVYDEDRAELIEREIPMRRAMNAILRDAYIEDCERAVARWNKILEQEDCRLRIHLPS
                     VRFHREVGTFGRASFDPNGNLITKDEFESRREEFLPTRADRDYVKSVQFLVTEAGKFA
                     NWIAPPQHGIHGRPLDFEYVRL"
     gene            complement(2709..4358)
                     /gene="boxC"
                     /locus_tag="VEC56_06970"
     CDS             complement(2709..4358)
                     /gene="boxC"
                     /locus_tag="VEC56_06970"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HXV13932.1"
                     /translation="METNVTTERIRFSTHPSRYKHWKLEIDGPVAWLKMNVNEDEGLR
                     PGYKLKMNSYDLGVDIELDDAVTRLRFEHPEVSVVAIESLNDKVFCAGANIFMLSTST
                     HTHKVNFCKFTNETRINIEEATAKSGQKYVASLNGAAAGGGYELALACDEIHLVDDRS
                     AAVSLPEVPLLGVLPGTGGLTRLVDKRKVRRDIADEFCTLAEGAKAKKALDWGLVDFI
                     HAKSVYAEAIKKRVTELAGRGHSAEGITLDDVEPEIDGDTLRWRYVTLEMQPAERMGK
                     ITIHGPSADDSMIPSEPSTLGAGWYALRVWRELRRAILELRFNYVSIGLITFHTEGDL
                     THVLALDEALEKRRDHWFIREVFLQQARTLKMIDLTARTFFAIVTPGSCFAGSLFEVA
                     LAADRIYMKDDAETECAIALSHANAGKNPMAHGLSRLENRFYGSPERVAKALEHQGEA
                     ITPDEARELGLATDVLDDIDWDDEIRMKIEERRSLSPDALTGMEANLRFVGPETMESR
                     IYARLSAWQNWIFTRPNAVGEKGALTSYGKPHSPEFDWSRV"
     gene            complement(4364..5398)
                     /locus_tag="VEC56_06975"
     CDS             complement(4364..5398)
                     /locus_tag="VEC56_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXV13933.1"
                     /translation="MKAITFEKHGGPEVLKLGDHPDPVTRPGTVKIDVHAASLNHIDV
                     FLRGGLPGINIPFPHIPGCDAAGVIREVGEGVTGLKTGDRVLMNPTIVCGKCEFCLRG
                     DATMCLDYHLIGETTSGTCCEQIVIPAENAIPIPNSMSFVDAASVPLVFVTAWRMLIT
                     RARLRAGEDVLILGATAGVGIACIQIAKVAGARVFAAASSEEKLALAMKLGADILINY
                     SKEDFPKKIRDLTAKRGVDVVVDYVGKDTWVKSLRSLSRGGRLVTCGATTGYNPEEDL
                     RHIFFRQLEVIGSTMGSKNELMAPLKLIWAGRMKPVVGAVYALKDTADAHRAMEERRV
                     MGKIVIKIRD"
     gene            complement(5395..5811)
                     /locus_tag="VEC56_06980"
     CDS             complement(5395..5811)
                     /locus_tag="VEC56_06980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV13934.1"
                     /translation="MTFSAEFSVRFGEIDQARVVYYPRFFHYFHQAFEEWFAGALGVS
                     YPDLVLKENIGFPSVRVETEFLKPLRYGDRIRVDLELLEIGTTSITVGYTLVRLTDGA
                     VSARATIKNVAINNDTFAPVAISDEWRRRFERFKNA"
     gene            complement(5808..6143)
                     /locus_tag="VEC56_06985"
     CDS             complement(5808..6143)
                     /locus_tag="VEC56_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXV13935.1"
                     /translation="MSELAKKHCIPCRGGVPPLKGDELRALSAKLGGGWSVVDEHHLE
                     KTFAFEDFRQALDYTNKVGEIAEHEGHHPDIYLGWGKVRLTIWTHKIDGLTESDFILA
                     AKADEAYRA"
     gene            complement(6166..>7288)
                     /locus_tag="VEC56_06990"
     CDS             complement(6166..>7288)
                     /locus_tag="VEC56_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV13936.1"
                     /translation="SVLLFPENIAIHADSATTLVPATADYRTTFTIGSFSGCGIDSAL
                     VSLYGMPLGYAHLNVKSRDVVCPSPPNPSRGKVDLLDFGTLGASYTSPPKPYNPCVDY
                     VAAFGQINVGDFAAFGAHWNHSASGGGSAPESASIVVSGTVALEFEESHPLLGEHTLR
                     ATMSLEGVPAFKAAMFALRNENPKLEFMGWMEASGYPGETMTTETIRDGRREVFIGVL
                     DADATEGDVVLGTAEFRVVSDQQLALTDDDLALVTADLLSNTDQRSTLALSSLAVRRA
                     VTSVEYKNELAQNYPNPFNPTTTISFSLAQTADASLSIYDVRGALVKSLYNGRAERGI
                     HRIVWDGTSNGGQQVASGVYFYKLTAGSFADTKKMTILK"
BASE COUNT         1249 a         2328 c         2313 g         1398 t
ORIGIN      
        1 cgccaccgtc cacaagaggc acaatgctcc cgacacgtgc ggcgccgcca tcgaggtccc
       61 cgacgcaatc gcatagcgat cgtcggggaa ggtgctgatc acgtcggatc ccggtgcgcc
      121 gaggtcgacg ctggtaaggc cgtagttcga gaagaagctg cgttcgtcgt cgtggtcggt
      181 gctggccaca ctgatcacgt tgtcgaggtc gaagctcgcg gggtagaacg gggtgacgtc
      241 gttgttgagg ccggcgttgc ccgcccccgc cacgaacagc gcgcccgcct cggcggcctc
      301 gtcgatggcg gagcgcagcg ccagcgagaa gccgatgccg ccccagctgt tgttggtgag
      361 ccgcgcgccc atcgcggtgg cgtactcgac cgcgcgaatc gcgtccgagg tggtaccgga
      421 gcccaccgcg ctcaggaact tcagcggcag gatgcgcgcg gaccagctca ctcccgcgac
      481 cccgatcgcg ttgttcccga ccgccgcgac gatgcccgcc acgtgggtcc cgtggccgtt
      541 gtcgtcgtcg gggtcgttgt cctcgttgac gaagtcccag ccgcgcacgt cgtcgatgta
      601 gccgttgccg tcgtcgtcga tgcggttgtc cgcgatctcc ctcgtgttca cgaagatgtt
      661 cggcgcgaga tcgacgtgct gccagtcgac gccgctatcg atgatgccga cgaggacgtc
      721 gccgccggtg acgtaggtcc acgcttcggg ggcgtcgacg tcggcgtcgg gcgtcccgcc
      781 ggtctggccg gtgttgtgca ggttccattg atcggcgaag tgctcgtcgt tgggaaacac
      841 gtcgaccgcg tggacgatgt agttgggctc gacgaagacg atgcgcgggt cgccttgcag
      901 gcgcgcgatc gcttgcggca cgccgatccc ggctatctcc cacaactcgg cgccgatgct
      961 atggaagcgg gctttcacgg tcgcgttgac gctcgttcgc aacgacgccg catccaccgt
     1021 gcgcgaggcg ggcgcgagct tgacgatgac ttggccggga acgaattcgt tgacctgggc
     1081 gtgcgcaacc ggcacgcccg ccgcggcgag cagaacgagc agtccgcgga cgagtccgcg
     1141 tgcaagacga gtacgtggac ggcggcagcg gacgacgacg tcaagccgag gtgtctcgac
     1201 cacggatctc ctagcaggtc ggcgagggat ggggtccgca gcccctattg tacgagcgaa
     1261 cgcgccgcgc tgtccagaac gccgcgcgtc ctacagacgc acgtattcga agtcgagcgg
     1321 gcgtccgtgg atcccgtgct gcgggggcgc gatccagttc gcgaacttgc ccgcctccgt
     1381 caccaggaac tgcacgctct tgacgtagtc gcggtccgcg cgcgtgggca ggaactcctc
     1441 gcggcgcgat tcgaactcgt ccttcgtgat caggttcccg ttggggtcga acgacgccct
     1501 gccgaacgtc cccacctcgc ggtggaagcg caccgagggc agatggatac gcagccggca
     1561 atcctcttgt tcgagaatct tgttccagcg cgccaccgcg cgctcgcagt cctcgatata
     1621 ggcgtcgcgc aggatcgcgt tcatggcgcg ccgcatcggg atctcgcgct cgatcagctc
     1681 ggcgcgatct tcgtcgtaaa ccgtgtagct gtagttgccc tcgcgcgccg acgcatccgg
     1741 gtagagcttg cggttggctt cgttgaaacg ccccttcaag ccgtgcgcga agtagttggc
     1801 cgcgttgctc gaatcctcat tgccgaaaag gtcgagggag accgagtacc aatagttgat
     1861 gtacttctgg acgatgtcga gcgggatgcc gccgtagggg cgcacgtctt cgctctcgtt
     1921 ctctttcatg agctgcgcgg tacggcgtat cgtgcgcgcg agccccgtct cacccacgaa
     1981 caggtggtgc gcttcctcgg tcagcatgaa acgcgtggtg cgcgagagcg gcgtgaatgc
     2041 gctctccgcc agcgacccca gctggtactt gccgtcgcgg tcggtgtagg tcgcgaagca
     2101 gaagaagtcg agccagtcgt cgatcggatc gttgaaggtc gacaagaggc gcggtgtgtc
     2161 cgggtcgccc gagcgacgct ccagcatctc ttccgcttcg tcgcggccgt ctttgccgaa
     2221 ataagagtgg agcagataga ccatcgccca aagatggcgc gcctcttcga cgttcacctg
     2281 gaacagattg cgcatgtcgt agagcgaggg cgccgacaac gcgagacgcc gctgctgctc
     2341 caccgagccc ggctccgtgt cgccctgggt cacgatcaag cgcttcaatt cgttgcgatg
     2401 ctcgcccggc acgtcttccc agcacccgtc gccgatgtag tcgccgaagt ggatcgaccg
     2461 ctcgctcttc acaggggcca ggaagatccc ccagcgatac tccggcatgc gcacgtagtc
     2521 gtacaccgcc cagcccgacg aatcgacgct caccgccgtg cgcagataga tctcgcgctc
     2581 ctggtgcgcc accggcccct ggtcacgcca ccattccagg aagcgcgggt gccagcgctc
     2641 cagcgcgcgc aagagcttgg ggttctcctt caggttgacg ttgttgggaa tcagctcttt
     2701 gtagtccatc agaccctgct ccagtcgaat tcgggtgaat gcggcttgcc gtacgatgtg
     2761 agggcaccct tttcgcccac ggcgttgggc cgggtgaaga tccagttctg ccacgcggac
     2821 aagcgcgcgt agatgcggct ctccatggtt tcggggccga cgaaacggag gttggcctcc
     2881 atgccggtca gcgcgtcggg agagagcgaa cgtctctcct cgatcttcat gcgaatctcg
     2941 tcgtcccagt cgatgtcgtc gaggacgtcg gtggcgaggc cgagctcgcg cgcctcatcg
     3001 ggcgtgatcg cctcgccttg gtgttcgaga gctttcgcga cgcgctcggg cgagccgtag
     3061 aaacgattct cgaggcgcga gaggccgtgc gccatggggt tcttgccggc gttggcgtgg
     3121 ctcaatgcga tcgcgcactc ggtctcggcg tcgtccttca tatagatgcg gtccgcggcc
     3181 agtgccactt cgaacaggct ccccgcgaaa cacgagcccg gcgtcacgat ggcaaaaaac
     3241 gtgcgcgcag tgaggtcgat cattttcaag gtgcgcgcct gctgcaggaa gacctcgcgg
     3301 atgaaccagt gatcccggcg cttctccaac gcttcgtcca aggccaggac gtgcgtgagg
     3361 tcgccctcgg tatggaatgt gatcaagccg atcgacacat agttgaagcg cagctcgagg
     3421 atcgcgcgtc gcaattcgcg ccacacgcgc agcgcgtacc agcccgcgcc cagcgtgctg
     3481 ggctcactcg ggatcatcga atcgtcggcg ctcggaccgt ggatggtgat cttgcccatg
     3541 cgctcggcgg gttgcatctc gagggtgacg tagcgccagc gcagcgtgtc gccgtcgatc
     3601 tcgggctcga cgtcgtcgag ggtgatgcct tcggcggagt ggccgcgccc cgcgagctcg
     3661 gtcacacgct tcttgatggc ctcggcgtag acgctcttgg cgtggatgaa gtcgaccaat
     3721 ccccagtcga gcgccttctt ggccttggcc ccctcggcga gggtacaaaa ctcgtccgcg
     3781 atgtcgcggc gcaccttgcg cttgtcgacc aggcgcgtga gaccgccggt gcccgggagc
     3841 acgccgagca acgggacttc cggcagacta accgcggcgc tgcgatcgtc cacgaggtgg
     3901 atctcgtcgc acgcgagtgc cagctcgtag ccgccgccgg cggcggcacc attcaaggac
     3961 gccacgtact tctggccgct cttggcggtc gcctcttcga tattgatgcg cgtctcgttg
     4021 gtgaacttgc agaaattgac cttgtgggtg tgcgtcgagg tgctcaacat gaagatgttg
     4081 gcgccggcgc agaacacctt gtcgttcaag ctctcgatgg ccaccaccga cacctcgggg
     4141 tgctcgaagc gcaggcgcgt caccgcgtcg tcgagttcga tgtcgacgcc caagtcgtac
     4201 gagttcatct tgagcttgta tccgggtcga agcccctcgt cctcgttgac gttcatcttg
     4261 agccacgcca ccggcccgtc gatctcgagc ttccagtgct tgtagcgcga cgggtgggtg
     4321 gagaatcgga tgcgctcggt tgtgacgttg gtttccatgg atatcagtcc ctaatcttga
     4381 tgacgatttt ccccatgacg cgccgttctt ccatcgcccg gtgcgcgtcg gcggtgtctt
     4441 tcaatgcgta tacggcgccg acgaccggct tcatgcggcc ggcccagatc aacttgagcg
     4501 gcgccatgag ctcgttcttc gaacccatgg tcgacccgat cacttccagc tggcgaaaga
     4561 agatgtggcg caaatcttcc tcgggattat accccgtcgt cgccccgcac gtcaccagcc
     4621 gcccgccgcg ggagaggctg cgcaagctct tgacccaggt atctttaccg acgtagtcga
     4681 cgaccacgtc gactccccgc ttggccgtca agtcgcggat cttcttgggg aagtcttctt
     4741 tcgaatagtt gatcaggatg tcggcgccga gcttcatggc cagcgcgagc ttttcctcgc
     4801 tgctggcggc ggcgaacacg cgcgccccgg ccaccttcgc gatctggatg cacgcgatac
     4861 cgaccccggc ggtggcaccc aggatcagca cgtcttcgcc cgcgcgcaga cgtgcgcgcg
     4921 tgatcagcat gcgccacgcg gtcacgaaca ccagcgggac cgacgccgcg tccacgaacg
     4981 acatcgaatt cgggatggga atcgcgttct cggccgggat cacgatctgc tcgcaacacg
     5041 taccgctcgt ggtctcgccg atcaagtggt agtcgagaca catggtcgcg tcgccgcgca
     5101 aacagaactc gcacttcccg cacacgatgg tcgggttcat caacacgcgg tcgccggtct
     5161 tgagtccggt gacgccctcg cccacttcgc ggatcacacc ggccgcgtcg cagcccggaa
     5221 tatgcggaaa cgggatattg atcccgggca aaccgccgcg caggaacacg tcgatatgat
     5281 tgagcgaggc ggcgtggacg tcgatcttga ccgtgcccgg gcgcgtgacc ggatccgggt
     5341 gatcgcccag cttcaggacc tcggggccac cgtgcttctc gaaggtgatc gccttcatgc
     5401 gttcttgaat ctctcgaagc gccggcgcca ctcgtcggag atggccacgg gcgcgaaggt
     5461 gtcattattg atggccacgt tcttgatggt cgcgcgcgcg ctgacagcac cgtcggtcaa
     5521 tcgtaccagc gtgtaaccca ccgtgatcga ggtcgtcccg atctcgagca gctccaggtc
     5581 gacgcggatg cggtcgccgt agcgcagggg cttcaagaac tccgtctcca cgcgcacgct
     5641 gggaaagcca atgttctctt tcaataccag atcgggatag gaaacgccga gcgcccccgc
     5701 gaaccactcc tcgaaggcct ggtggaaata atggaagaaa cgcgggtaat agacgacgcg
     5761 ggcctggtcg atctcaccga agcgaacgct gaactcggcg ctgaacgtca tgcgcgatag
     5821 gcttcgtcgg ctttggcggc cagaatgaaa tcgctctcgg tcagcccgtc gatcttgtgg
     5881 gtccagatgg tgagccgcac cttgccccac ccgagataaa tgtccgggtg gtgtccctcg
     5941 tgctctgcga tctcgcccac cttgttggtg taatcgagcg cctgccggaa atcttcgaac
     6001 gcgaacgtct tctcgaggtg gtgctcgtcc accacgctcc agccgccgcc gagcttggcc
     6061 gaaagcgcgc gcagctcatc acccttgagg ggtggaaccc cgcctcggca ggggatgcaa
     6121 tgtttcttgg cgagttcact cataggtttg gattggccca ggtgctcact tcagaatcgt
     6181 catcttcttc gtgtcggcga acgagccggc tgtcaacttg tagaagtaga cgccgctcgc
     6241 tacctgttgc ccgccgttgc ttgtaccgtc ccagacgatg cggtggatcc cgcgctccgc
     6301 ccgtccgttg tacaacgact tcaccagcgc accacgaaca tcgtagatcg acaggctggc
     6361 atccgcggtc tgcgccagcg aaaacgaaat tgtcgttgtc gggttgaacg gatttgggta
     6421 gttctgcgcc agctcgttct tgtattcaac cgacgtcacg gcgcgccgca cggccagaga
     6481 gctcaaggcc agcgtgctcc gctgatccgt gttggaaaga aggtcggccg tcacgagcgc
     6541 gaggtcgtcg tccgtgagag ccagctgctg gtccgaaacg acgcgaaact ccgccgtccc
     6601 gagcaccaca tcgccttcgg tcgcgtccgc gtccagcacg ccgatgaaga cctcccggcg
     6661 tccgtctcga attgtctcgg tggtcatcgt ctcgccgggg tatcccgaag cctccatcca
     6721 ccccatgaac tccagcttgg ggttctcgtt gcgaagcgcg aacatcgcgg ccttgaacgc
     6781 aggcacgcct tcgaggctca tcgtcgcccg caatgtgtgc tcgccaagaa gcggatgcga
     6841 ctcctcgaac tcgagtgcga ctgtccccga tacaacgata ctcgccgatt cgggcgccga
     6901 tcctcctccc gatgccgaat ggttccagtg agcaccaaac gccgcaaagt ctcctacgtt
     6961 gatctggccg aacgctgcga cgtagtcaac acacgggttg tacggtttcg gtggcgaggt
     7021 gtagcttgcc ccaagagtgc cgaagtctag aagatcaacc ttgccgcggc ttgggtttgg
     7081 cggcgatggg cagacgacgt cacggctctt gacgttgaga tgtgcgtagc ctagcggcat
     7141 gccatacaag ctcacgagcg cggagtcgat gccgcatccg ctaaacgaac caatggtgaa
     7201 ggtggttcta taatctgcgg ttgccggcac gagagttgtt gcagaatctg cgtggatagc
     7261 aatgttctcg ggaaacagca atacggat
//
LOCUS       DASPGK010000257         7015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1588309, whole genome shotgun sequence.
ACCESSION   DASPGK010000257 DASPGK010000000
VERSION     DASPGK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588309"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VEC56_06995"
     CDS             <1..1213
                     /locus_tag="VEC56_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13937.1"
                     /translation="WGDELSLAQENVYTDADRMVGIKETMGGKAIAGYALTRDMPLGL
                     TKIEALGSAGSDGQFVGAIELRLSWGRSTDVLIKAETFSSELEIDEEIAGSAFPFDFP
                     FDTDRWFARFEAGASSALRLRAWGLYETNSGEGDVVQGFENRLWVRRAGGGVSVDYKL
                     EPWYRLYLVPRRTGATARGPGLRLRFEYTEVDSDLEMRFNDVRYMHLDGLQGADWIGR
                     LDVMPWSFLSLFGGWERVQLEHHGDSFFDVWPFVIWDVFTARRYRLDDMESTLDTWYV
                     GSAARHESTHILAEVSGRFEWWDSAAQLDWLERVDVLFPFFFRYERHTESSSLDVDYA
                     VQVDAALWWRFSGASLRLAGRATIPLQNDEDPDPAGPGGPPSPGGPPPPPSEDSSHGG
                     LSGTIELVLGR"
     gene            complement(1229..2848)
                     /locus_tag="VEC56_07000"
     CDS             complement(1229..2848)
                     /locus_tag="VEC56_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXV13938.1"
                     /translation="MTDSTAPSSRVGIAACVVACGCLVGVLASATSRADPPRRDFKLE
                     SEQVYHFSRNFSSPTRIELASLQYLLTPDELLDLLSLRNESSCIRWMSEFWRDRDPIL
                     TTPENEARQEHERRVEFATSWFGRAEWPGWDQRGEVCIRYGLPGGRDIVTADVSPDSY
                     VRPGEYWFYPAFGMTVQFEDAFGNGNYTYYLERVALPIGERWSSDRLRMPAGQWKQMP
                     DLDLDLMTLDVVLGAKGGYFGMYGSSDQFTYEDFQQSLWRFPEVLETTPAVFPFDFEH
                     LRVPFEFDVAYFRGGEALDRVDVNAEFEAVDRSRPGATQYRATAVFFDLDAKEIARVS
                     HTTNVLRDPATADSLFDVVMQLPSTLSPGLYEMAITIEESGTGRFSSYRKNVSLDDFS
                     RRLAMSTVCFTSRIEPVTRPSAFNRGALEVVPKPSARYDVATSVPVYFEVYNLTADGE
                     GLHRYQVSYRVMPLTPAPKGLWKKIVGGSEEATVLSSSFESAAAGPHDVVYVFLKTDE
                     LWPGEFEFDVTVVDAVSLQETARRGRFRIVE"
     gene            3076..3525
                     /locus_tag="VEC56_07005"
     CDS             3076..3525
                     /locus_tag="VEC56_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV13939.1"
                     /translation="MKLIRGQETWDPFRDVDDLATRVNRLFGLTKWNGESLAMADWVP
                     SCNVSEDEKEYRVRAELPDVKKDDVHVTLENGVLTIEGERKEEKEEKDLKFHRRELSY
                     GKFVRRFAMPEEADESKIQASFHDGMLEVTIAKSKVKAPKYKEIPVA"
     gene            3552..3917
                     /locus_tag="VEC56_07010"
     CDS             3552..3917
                     /locus_tag="VEC56_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HXV13940.1"
                     /translation="MKIPLQVTFRNVARSDLIEAYVRERAESLEKYAEQVVGVRVVVE
                     SPHRRHRKGQLFHVRVEVAVPGGDLIIRRDPAEKHAHEDVFVATRDAFAAARRRLQDY
                     ARRRRGDIKRHVPQAAARR"
     gene            3931..5259
                     /locus_tag="VEC56_07015"
     CDS             3931..5259
                     /locus_tag="VEC56_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXV13941.1"
                     /translation="MRFENREDAARRLVQKLARFRGQHPLVLAIPRGAAIMGRVMADE
                     LNGEMDIVLVRKLRAPGQPELAIGAVDEHGHMFLTGYPSDVSDLYLKQEKQTQMEALK
                     RRRAVYGRAAANPRGRVVIVVDDGVATGSTMIAALKSVREAKPAHVIAATAVAPPETV
                     DRLREFADEIVCLSSPGDFRAVGQYFDDFGQVSDDEVVALLKGEVRSFAPAVDPEITI
                     DVRGRRLRGILARPADSKGVVVFAHGSGSSRLSSRNRHVAEVLNQHGIATLLFDLLTP
                     EEGRVHDMRFDVGLLGVRLVEVTEWVADRPDLKGLPIGYFGASTGAAAAMIAAARLGG
                     KIACIVSRGGRPDLAMEVADHVFAPTLLIVGGDDREVLELNRKVYDVIPGVKELSVVR
                     GASHLFEEPGMLDDVARRAVAWFHDHLETPAIIPSSHAHEGGTPHGLVTR"
     gene            5240..5674
                     /locus_tag="VEC56_07020"
     CDS             5240..5674
                     /locus_tag="VEC56_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV13942.1"
                     /translation="MASLLVRDLMTEKVYSIHPGEDLARLVDLMDDIHVRHVPVVDDQ
                     RIIVGLVSQRDLVREVLHATRDLPFSHVRELLKHKNVRSIMTTDVETVEPDTKVDEAG
                     QIMLDNKLGCLPVVEGDRLVGILTEADFVKFTVGLAQRTPAA"
     gene            complement(5637..>7015)
                     /locus_tag="VEC56_07025"
     CDS             complement(5637..>7015)
                     /locus_tag="VEC56_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13943.1"
                     /translation="WGSPGEVAAVGNLGTAAHLVVAPWTTKDSGTFNDLCDVWMADDA
                     RAFAVGLGGTILLYDGAWSASASGTAVNLADVFGFEDGTAVAVGEAGTILRYDGSAWS
                     PMASVTSVNLFGVWGSAPDNIYAAGVGGTIVHYDGGAWSFETTATPENLSAVWGSSAS
                     DVWAVGDSGAIMRFDGATWALSPSGTTATLGDVWGSSASDIWAVGSAGTIRHYDGAAW
                     STVESGTESNLLGVWGKSACEVFAVGAAGTILRYGPDDESGVHTFVCHDHPQGAVAPP
                     TYGLRLDDLLGEGQYTFSFDYTDGAESARVTLTHDEAAGTVRIAGRAYGGLLEGDEWS
                     SEASGWIDFDFTYTDAVAVYDNCARDAGDDVYVTGASALNAGTFTLVGWGGDASFDFI
                     DRADEHGCSFVFDNDWDSKGVGGIADDPNVWSAAGWLQPGTDGSRDWLFIARPVVTPR
                     ECAAPSRP"
BASE COUNT         1280 a         2211 c         2314 g         1210 t
ORIGIN      
        1 gtggggggac gagttgtccc tcgctcaaga gaacgtctac accgacgccg accgcatggt
       61 tgggatcaag gagaccatgg gcggaaaggc gatcgccggg tacgccctca cgcgcgacat
      121 gccactcggt ctgacgaaga tcgaagcgct ggggtcggcc ggaagcgacg ggcagttcgt
      181 cggtgccatc gagttacggc tttcctgggg gcgctcgacc gacgtgctga tcaaggccga
      241 aaccttctcg agcgagttgg agatcgacga ggagatcgcg ggcagtgcct ttccgttcga
      301 ctttcccttc gacaccgacc gatggttcgc gcgcttcgaa gcgggcgcgt catcggcgct
      361 gcgattgcga gcgtggggac tctacgagac caacagcggc gaaggcgacg tcgtgcaggg
      421 cttcgagaac cgactctggg ttcgacgcgc gggcggtggg gtctccgtcg actacaagct
      481 cgagccgtgg taccggctct acctcgttcc gcgccggacg ggtgcgacag ctcgcgggcc
      541 cggtctgcgt ctgcgcttcg agtacaccga ggtcgacagc gacctcgaga tgcgttttaa
      601 cgacgtacgc tacatgcacc tggacggtct ccagggcgcc gactggatcg gacgtctgga
      661 cgtcatgccg tggagcttct tgtcgctatt cggcgggtgg gagcgggtcc aactcgaaca
      721 ccacggagac tcgttcttcg acgtgtggcc gttcgtgatt tgggatgtct tcacggcccg
      781 gcgctatcgc ctcgacgaca tggagagcac gctcgacacc tggtatgtcg gaagcgccgc
      841 gcgccacgag tcgacgcaca tcctggccga ggtgtcgggt cggttcgagt ggtgggatag
      901 cgcggcacaa ctcgattggc tcgagcgagt cgacgttctg tttccgttct tctttcgata
      961 cgagcgccac accgagtcat ccagtttgga tgtggactac gcggtgcagg tggacgcggc
     1021 gttgtggtgg cgcttcagtg gagcgtcgct acgcctggcc ggacgcgcga cgattccgct
     1081 ccagaacgac gaggatcccg acccagccgg gccgggtggt ccgccgagcc cgggaggacc
     1141 tcctccacct ccgagcgagg actcgtcgca cgggggactg tcggggacga ttgaattggt
     1201 gctcggacga tagaaaatcg ccgcggcgct actcgacgat gcgaaagcgt ccccggcggg
     1261 cggtctcctg caacgacacc gcgtccacca cagtgacgtc gaattcgaat tcacccggcc
     1321 acagctcgtc ggtcttgagg aacacgtaga cgacgtcgtg gggtcccgcg gccgcggact
     1381 cgaagctcga cgagagcacg gtcgcctcct cggagcctcc cacgatcttc ttccagagcc
     1441 ccttgggcgc gggcgtcagc ggcatgacgc ggtaggatac ctggtaacga tggagcccct
     1501 cgccgtctgc ggtgaggttg tagacctcga agtacaccgg taccgacgtc gccacgtcgt
     1561 aacgcgcgga cggcttcggc acgacttcca acgcaccgcg attgaacgcc gatggccgcg
     1621 tgaccggttc gatccggctc gtgaagcaca ccgtgctcat cgcgagtcgc cgggagaaat
     1681 catcaagcga gacgttcttg cgatacgacg agaatcggcc cgtgccgctc tcctcgatcg
     1741 tgatcgccat ctcgtagagc ccgggggaga gcgtcgaagg gagctgcatg acgacgtcga
     1801 agagcgaatc ggcggtcgcg gggtcccgta gcacgttggt ggtgtgcgag acgcgcgcga
     1861 tctccttggc gtccagatcg aagaagaccg cggtcgcacg atactgcgtg gctccgggtc
     1921 gactgcgatc gaccgcttcg aattcggcgt tcacgtccac ccgatcaagc gcttcaccgc
     1981 cgcgaaagta cgcgacgtcg aactcgaacg gcacgcgcaa gtgctcgaag tcgaagggaa
     2041 acacggccgg tgtggtctcc aggacttcgg ggaaacgcca cagggattgc tggaagtcct
     2101 cgtacgtgaa ttggtcggat gacccgtaca tgccgaagta gccgcccttc gcgcccaaga
     2161 cgacgtccaa cgtcatgaga tccaggtcca ggtcgggcat ttgtttccac tgcccagcgg
     2221 gcatgcgcaa gcgatcgctg ctccagcgct cgccgatcgg caacgccacg cgttcgagat
     2281 aataggtgta gtttccattc ccgaacgcat cctcgaattg aacggtcatt ccaaacgccg
     2341 gatagaacca gtactcgccc gggcgaacgt aggaatcggg actgacgtcc gccgtcacga
     2401 tgtcccttcc gccggggagc ccgtagcgaa tacagacttc gccgcgctgg tcccagcccg
     2461 gccactccgc gcgcccgaac caactggtcg cgaattcgac gcggcgctcg tgctcctgcc
     2521 gggcttcgtt ctcgggtgtg gtcagaatgg gatcgcggtc gcgccaaaac tcgctcatcc
     2581 agcgaatgca gctcgattcg tttcggagcg agaggaggtc cagcaactcg tccggcgtga
     2641 gcaggtactg caacgaggcc aactcgattc gagtaggcga ggagaagttg cggctgaagt
     2701 ggtagacctg ctcgctttcg agtttgaaat cgcgacgggg cgggtcggcg cggctggtcg
     2761 cgctcgcgag tactccgacg agacatccgc acgccacgac gcacgcggcg atgcccacgc
     2821 ggctactggg ggcggtcgaa tcggtcacgt gcgctccccg cagttcgggt cctggatcgg
     2881 tcgggcagcc cgacggcatc actataaaga ctcccccccg atcggggcaa gcaattcagt
     2941 ggcttacaac atatttcaca atgtgttacc gcggcgttga tattttaaga tgtttttatc
     3001 ctgtgcctgc gtatactccc attaggagac tgcccgcgag gcgaacgccc gcgggaggca
     3061 aaggagggtt caatcatgaa actcatacga ggccaggaga cctgggatcc gtttcgcgac
     3121 gtcgacgacc tcgcgactcg ggtcaatcgg ttgttcgggc tgaccaagtg gaatggcgaa
     3181 tcgctcgcga tggcggattg ggttccgtca tgcaacgtca gcgaggacga gaaggagtat
     3241 cgcgttcgcg ccgagcttcc ggacgtcaag aaagacgacg tgcacgtgac gctcgagaac
     3301 ggcgttctca ccatcgaggg cgagcgcaaa gaagagaagg aagagaaaga cctcaagttc
     3361 caccggcgcg agttgagcta cgggaagttc gtccgtcgtt tcgccatgcc ggaggaagcc
     3421 gacgagtcga agatccaggc gagcttccat gacggaatgc tcgaggtgac catcgccaag
     3481 tcgaaggtga aggctcccaa gtacaaggag atccccgtcg cgtagatgcg gaagagggag
     3541 gatcgtgagc aatgaagatc ccgctgcagg ttactttccg aaacgtggct cggtcggatc
     3601 tgatcgaagc gtacgtgcgt gagagggccg agtccctcga gaagtacgcg gagcaggttg
     3661 tgggcgttcg cgtagtggtg gaaagcccgc accgtcgcca ccggaaaggg cagctcttcc
     3721 atgttcgcgt cgaggtggcg gttcccggcg gagacctcat catccgccgg gatcccgccg
     3781 aaaagcacgc ccacgaagac gttttcgtcg cgacccgcga cgcgttcgcc gcggctcgtc
     3841 gacgccttca agactacgct cgtcgccgcc gaggcgacat caagcgccac gtgccgcaag
     3901 cggccgcccg gcgctgaagg agtgacatcg atgagattcg agaatagaga ggacgctgcg
     3961 cgacgcctcg tgcagaagct tgcgcggttc cggggtcagc acccgctggt gctcgccatc
     4021 ccgcgcggcg ccgcgatcat gggccgcgtc atggcggacg aactgaacgg cgagatggac
     4081 atcgtcttgg tgcgcaaact gcgagcaccc ggtcagcccg agctcgccat cggagccgtc
     4141 gacgaacacg gccacatgtt cctgaccggc tacccgtcgg acgtctccga tttgtatcta
     4201 aaacaggaga agcagactca aatggaagcg ctgaagcggc gtcgtgcagt ttatgggcgc
     4261 gccgccgcca atccacgcgg gcgtgtggtc atcgtggtcg acgacggcgt tgccaccgga
     4321 tcgacgatga tcgccgcgtt gaagtcggtg cgggaagcga agccggcgca tgtgatcgcc
     4381 gccaccgcgg tggcgccgcc cgaaaccgtc gatcgtctgc gcgagttcgc ggacgaaatc
     4441 gtgtgcttgt catcgcccgg cgatttccgc gccgtgggac agtacttcga cgacttcggt
     4501 caagtgagcg acgacgaagt agtcgcgctg ttgaagggcg aagtccgatc attcgcaccc
     4561 gcggtcgacc ccgagataac gattgacgtc cgcggccggc ggctgcgtgg cattctggcg
     4621 aggcccgcgg attcgaaggg tgtggtcgtg ttcgcacacg gcagcgggag cagccgcttg
     4681 agttcgcgca accgccacgt ggcggaagta ctgaaccaac acggtatcgc gacacttctg
     4741 ttcgacctgc tcacccccga agaaggccgc gtccacgaca tgcgtttcga cgtggggctg
     4801 ttgggcgtgc gtctggtcga ggtaaccgag tgggtcgcgg accggcccga tctcaagggt
     4861 cttcccatcg gctatttcgg cgcgtcgacg ggggccgcgg cggccatgat cgcagccgcg
     4921 agactcggcg gcaagatcgc atgcatcgta tcgcggggcg ggcgccccga tctggcgatg
     4981 gaggtcgcgg accacgtgtt cgctcccacg ctgttgatcg tcggcggtga cgatcgcgag
     5041 gtgctggaac tcaatcgaaa ggtctacgac gtgatccctg gggtcaagga gctatcggtc
     5101 gtgcgcggcg ccagtcacct gttcgaagag ccgggaatgc tcgacgacgt cgcgcgccgg
     5161 gcggtggctt ggttccacga tcacctggaa acgccggcca tcatcccgtc gtcgcatgca
     5221 cacgaaggag ggacgccaca tggcctcgtt actcgttagg gacttgatga ccgagaaggt
     5281 ctacagcatc catcccggcg aagacctggc gcgtctggtc gacttgatgg acgacatcca
     5341 cgtgcggcat gtccccgtgg tggacgacca gcgtatcatc gtgggattgg tatcgcagcg
     5401 cgatctggtg cgcgaagtgc tgcatgccac acgcgacttg ccgttctcgc acgtgcgcga
     5461 gctattgaaa cacaagaacg tccgctcgat catgaccact gacgtcgaga ccgtggaacc
     5521 ggataccaag gtcgatgagg ccggccagat catgctcgac aacaagctcg gctgccttcc
     5581 ggtcgtggaa ggggaccgct tggtcggcat actcacggaa gcggacttcg tgaagtttac
     5641 ggtcggcttg gcgcagcgca ctcccgcggc gtgacgaccg gtcgcgcgat gaagagccag
     5701 tcgcggctgc cgtcggtgcc cggctgaagc cagcccgcgg cggaccacac attggggtcg
     5761 tcggcaatcc cgccgacgcc cttcgaatcc cagtcgttgt cgaatacgaa cgagcacccg
     5821 tgttcgtccg cgcggtcgat gaagtcgaaa gacgcatccc cgccccaccc gacgagcgtg
     5881 aaggtgcccg cgttgagcgc gctcgcgccg gtcacgtaca cgtcgtcacc ggcgtcacgc
     5941 gcgcagttgt cgtacaccgc caccgcgtcc gtgtaggtga agtcgaagtc gatccagccg
     6001 cttgcttcgc tcgaccactc gtcgccctcg agcaacccgc cgtacgcgcg cccggcgatg
     6061 cgaaccgtcc cggcggcttc gtcgtgggtg agcgtcacgc gcgcgctctc cgcgccgtcc
     6121 gtgtagtcga agctgaaggt gtactggcct tctccgagga gatcgtcgag gcggaggccg
     6181 taagtgggag gcgcgaccgc gccttgcggg tggtcgtggc acacgaaggt gtgaacgccg
     6241 ctctcgtcgt cggggccgta gcgcagtatg gttcccgccg cgcccaccgc gaagacctcg
     6301 cacgcgctct tgccccacac gccgaggaga ttgctctcgg ttccgctctc caccgtgctc
     6361 cacgcggcgc cgtcgtagtg gcgaatcgtg cccgcggagc cgacggccca gatgtcgctc
     6421 gccgatgatc cccacacgtc gcccagagtg gccgtcgttc ccgacggcga gagcgcccac
     6481 gtcgcgccgt cgaagcgcat gatggcgccg ctgtcgccca ccgcccacac gtcgctcgcc
     6541 gacgatcccc acaccgcgga aagattctcc ggggtggcgg tggtctcgaa cgaccacgcg
     6601 ccgccgtcgt agtggacgat ggtcccgccc acgcccgcgg cgtagatatt gtccggtgcg
     6661 ctgccccaca cgccgaagag attgacactc gtcacactcg ccatgggcga ccacgcgctg
     6721 ccgtcgtagc gaagaatcgt ccccgcttcg cccaccgcga ccgcggttcc gtcctcgaag
     6781 ccgaagacgt cggcgagatt gacggcggtc ccgctggccg aggccgacca ggccccgtcg
     6841 tagagcaaga tggtgccgcc cagaccgacc gcgaacgcgc gcgcatcgtc ggccatccag
     6901 acgtcgcaaa gatcattgaa cgtcccgctg tccttggtcg tccacggcgc gaccacgagg
     6961 tgcgccgcgg tgccgaggtt gccgaccgcg gctacttcgc cgggcgatcc ccaca
//
LOCUS       DASPGK010000258         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_886423, whole genome shotgun sequence.
ACCESSION   DASPGK010000258 DASPGK010000000
VERSION     DASPGK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886423"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VEC56_07030"
     CDS             complement(<1..1448)
                     /locus_tag="VEC56_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXV13944.1"
                     /translation="MVVAVLLGVVDANAQQSRPNILVIWGDDIGTWNISHNNRGMMGY
                     MTPNIDRIAKEGIAFTDYYGQQSCTAGRAAFIGGNVPVRTGMTKVGLPSAKEGWQKTD
                     VTMATVLKSLGYATGQFGKNHQGDRDEHLPTMHGFDEFMGNLYHLNAEEEPENEDYPT
                     NVALPDGRKFIDVFGPRGVLKCKADGKGGQTIEDTGPLTKKRMETIDDETVAAATDFI
                     KRQHQAGKPFFCWWNGTRMHFRTHVRDSHRHEGNDEYTDGMIEHDMNVGQLLKLLDDL
                     GIADNTVVLYSTDNGPHYNTWPDAGTTPFRSEKNSNWEGAYRVPAFVRWPGHFPAGVA
                     LNGIVAHEDWLPTFAAIGGDADIPAKLRKGVSLNGRKYKNYIDGYNLVDYLTGKSNES
                     PRKEFMYVNDDGQIVAIRYNVWKAIFLENRGVAFEVWREPFTHLRVPLLFNLRRDPFE
                     KAQHNSNTYNDWFLERVFVLAPMQQFAANFLLT"
     gene            complement(1495..2307)
                     /gene="purQ"
                     /locus_tag="VEC56_07035"
     CDS             complement(1495..2307)
                     /gene="purQ"
                     /locus_tag="VEC56_07035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HXV13945.1"
                     /translation="MASKVLIIQFPGVNCEYESLRVVESVGLRGEIRRWNDASAAVKN
                     APAIILPGGWSYQDRIRAGVVAAKDKVLDDVVAAAGRGVPVIGICNGAQVLVETGIVP
                     GFEPGRIDVALAPNQMTGRVGYYCNWAYVKKGPARCLFTDFMEEQPNTPLPIPLAHGE
                     GRFVTANQSVRAHFERGDGVALTYCTAGGATAAQFPENPNGAQFSIAALTNPNGNVLA
                     IMPHPERGAWAHQVPRRVGGTWGRSRDGVGSKDLYAAGPGRGFFESLRRALV"
     gene            complement(2310..>2756)
                     /locus_tag="VEC56_07040"
     CDS             complement(2310..>2756)
                     /locus_tag="VEC56_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HXV13946.1"
                     /translation="RWFGNHGRERAPQPDFAFERALVQALVAVFSKRLVLAAHDVSLG
                     GLLVTVAEMAIASAPFDVGAKIDLGGASAAACFAEMGGIVVEVSDGSWEECAAVLARH
                     SVPFLEIGRTIEAPALHVGTKAGSFTVTNQELRLAHAGRLAEILYG"
BASE COUNT          456 a          906 c          862 g          532 t
ORIGIN      
        1 gtcagcagga agttcgcggc gaactgctgc atcggcgcca gcacgaacac gcgctccagg
       61 aaccagtcgt tgtacgtgtt cgagttgtgc tgcgccttct cgaacggatc gcgccgcaga
      121 ttgaacaaga gcggcacgcg caagtgcgta aacggctcgc gccacacctc gaaggcgaca
      181 ccgcggttct ccaggaaaat cgccttccac acgttgtaac ggatagcgac gatctggccg
      241 tcgtcgttga cgtacatgaa ctctttgcgc ggtgattcat tgctcttgcc ggtcaagtaa
      301 tcgaccaaat tgtacccgtc gatgtagttc ttgtacttgc gaccgttgag cgagactccc
      361 ttccggagct tggcggggat gtcggcgtcg ccgccgatgg cggcaaacgt gggcaaccag
      421 tcctcgtgcg ccacgatgcc gttgagggcg acacccgccg ggaagtgccc cggccagcgc
      481 acgaacgccg ggacgcgata ggcgccctcc caattggagt tcttctcgct gcggaacggc
      541 gtcgtgcccg cgtccggcca cgtgttgtag tgcgggccgt tgtcggtgga atacaacacg
      601 accgtgttgt cggcgatgcc gaggtcgtcg agcagtttca agagctgacc gacattcata
      661 tcgtgctcga tcatgccgtc ggtgtactcg tcgttgccct cgtgccggtg cgaatcgcgc
      721 acgtgggtgc ggaagtgcat gcgcgtcccg ttccaccagc agaagaatgg cttgcccgcc
      781 tggtgctggc gcttgatgaa gtcggtcgcc gccgcgacgg tctcgtcgtc gatggtctcc
      841 atgcgcttct tcgtgagcgg gccggtatcc tcgatggtct ggccgccctt gccgtcggct
      901 ttgcacttga gcactccgcg cggcccgaac acgtcgatga acttacggcc gtcgggcagc
      961 gctacattcg tgggatagtc ctcgttctcg ggctcttctt cggcgttgag gtgatagagg
     1021 tttcccatga actcgtcgaa gccgtgcatg gtgggaaggt gctcgtcgcg gtcgccctgg
     1081 tggttcttcc cgaactgccc ggtcgcgtag ccgagactct tcaggaccgt cgccatggtg
     1141 acgtcggttt tctgccagcc ctccttggca ctcggcagac ccaccttggt catgcccgtg
     1201 cgcaccggga cattgccccc gatgaacgcc gcgcgccccg cggtacagct ttgctggccg
     1261 tagtaatccg tgaaggcgat gccttccttg gcgatgcggt cgatgttggg ggtcatgtag
     1321 cccatcatgc cgcggttgtt gtggctgatg ttccacgtgc cgatatcgtc gccccagatc
     1381 acgagaatgt tgggcctcga ctgctgtgcg ttcgcgtcca cgacgcccag caggacggca
     1441 accaccatca cgcttgcgag tattgctctc atggctccct ctcttcggtt cgagttacac
     1501 caacgctctt cgtagcgatt cgaaaaatcc gcggccgggt ccggccgcat agagatcctt
     1561 ggatcccaca ccgtcccggc ttcggcccca ggtcccgccg acgcgcctag gcacctggtg
     1621 cgcccacgca ccccgctcgg ggtgcggcat gatcgcgagc acgttgccat tcggattggt
     1681 cagtgcagcg atggaaaact gcgcgccgtt ggggttctcc ggaaattgcg ccgcggtggc
     1741 acctccggcc gtgcaatacg tgagcgccac accgtcgccc cgctcgaagt gcgcgcgcac
     1801 gctctggttg gccgtcacga agcgtccctc gccgtgcgcg agcggaatcg gcagcggcgt
     1861 gttgggctgc tcctccatga aatcggtaaa caggcagcgc gcgggcccct tcttcacgta
     1921 cgcccagttg cagtagtaac ccacgcgccc cgtcatctga ttgggcgcga gtgcgacgtc
     1981 gatgcgcccg ggttcgaagc cgggcacgat gccggtctcg accaatactt gcgcgccgtt
     2041 gcagatgccg atcacgggca caccgcggcc ggcggccgcg accacgtcgt cgagcacctt
     2101 gtccttggcc gccaccacac ccgcgcgaat gcgatcctgg taggaccacc cgccgggcag
     2161 aatgatcgcg ggcgcgttct tgaccgccgc cgacgcgtcg ttccaccggc gaatctcacc
     2221 gcgcaggccg accgattcga ccacgcgcaa gctctcgtac tcgcagttga cgccgggaaa
     2281 ctgaataatg aggactttcg acgccatggt tatccgtaga ggatctccgc gagccgcccc
     2341 gcgtgcgcca agcgcaactc ctggttggtg acggtgaacg aaccggcttt ggtgccaacg
     2401 tgcaacgcgg gtgcttctat ggtacggccg atctcgagaa aaggaaccga gtggcgtgcg
     2461 agcacggccg cacattcttc ccacgatccg tcgctcactt cgaccacgat tccgcccatt
     2521 tcggcgaagc acgcggcggc cgacgcgccg ccgagatcga tcttcgcgcc cacgtcgaag
     2581 ggcgcgcttg cgatcgccat ctctgcgacg gtgacgagca atccgcccag cgacacgtcg
     2641 tgggccgcga ggacgaggcg cttgctgaat acggccacga gcgcctgcac cagcgcgcgc
     2701 tcgaacgcga aatcgggctg gggagctcgt tcacgaccgt gattgccgaa ccaccg
//
LOCUS       DASPGK010000259         7778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1120216, whole genome shotgun sequence.
ACCESSION   DASPGK010000259 DASPGK010000000
VERSION     DASPGK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120216"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VEC56_07045"
     CDS             <1..1099
                     /locus_tag="VEC56_07045"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXV13947.1"
                     /translation="GTSTNMNANEVIANRAAEILGKERGHKSIHPNDHVNMSQSSNDV
                     IPTAIHVAAATAITTELIPALEHLGLTLEAKAEQFDDVVKSGRTHLMDATPVRLGQEF
                     GGFERQVANAVERAARARDTLLELALGGTAVGTGINRRAEFPQVAIKFIGEDTALGFY
                     EAMDHFEAQGAKDACVEASGQMKTIAVSLSKIANDIRLMASGPRTGIAEITLPSIQPG
                     SSIMPGKVNPVMCESVTMVCAQVIGYDAAITQGGLGSYLELNVMMPLIAYDLLEQIRL
                     LSNVSRVFANKCIAGITANDSRARELLELNLSTCTSLAPKIGYDKAAALSKQAFATGR
                     TVRDVAREMKVLPEDELNRLLDYKRMTEPDA"
     gene            1382..1777
                     /locus_tag="VEC56_07050"
     CDS             1382..1777
                     /locus_tag="VEC56_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13948.1"
                     /translation="MRRLACIAVAAITILVTAGDDRAKESFASMVVPQAPAASGMVVQ
                     IDPATGLPVTPTPETMLALQDRMKLNRSTEGLVEVPSAVVGGGYTVDLQGRFQHSMTV
                     TIDASGVVHSTCTTSGTSPEEVTASCVDH"
     gene            1762..4326
                     /locus_tag="VEC56_07055"
     CDS             1762..4326
                     /locus_tag="VEC56_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HXV13949.1"
                     /translation="MRRPLMNCLIVLAAVLLAATPFDAQAAATIVIQNNDGAGEGFND
                     PTPFTPVGGNNAVTLGQARLIAFTYAANLWGGCLNSNVTITIRAQMDPQTCTPTSATL
                     GSAGATTVHRDFTNAPAANTWFSQALANSLNGADLDGGQPDVNATFNSSINGNPACLG
                     GGSWYYGLDGNPGPNQWDFVTVVLHEIGHGVGFQTFIDDNTGARFMDRNDQYMRFMEQ
                     AGATPSMYTAMNDAQRAAGNISDPNLRWTGGSANAEHPNVPITAGLNGGLLRLHGPNP
                     TQPGSSLSHWSTALSPSELMEPSYTAPDHNMSLALYLLEDIGWSIDDSPPTITCPSDV
                     DVECTTASGTPRSNPAIQAWLASVTTSGGCGEVVVTNDAPSFFPDGTTTVTFTARDGA
                     INTVTCTADVNVVDTTPPVIVCPTDVTVECSSHCGVANDDPQLAAFFAGVSATDICDP
                     SPVITHDAPACFPEGETTVTFTATDDDLNFITCTAKVTVEDTTPPEIDVVLSRDVLWP
                     PNHKLAEVCAEVTVTDVCDPNPTFVLTSIESDEPDNGQGDGNFPNDIQDAEYGTPDLC
                     FDLRSERQGGEDGRKYTIIYCASDEANNTACDTVCVRVPHDQGAGALSSTGFIVDGRG
                     FVSGSDQFAVIIPAIDGIDAGALETSRVYLGNTGGVAQPQEIRIVEVNSDGRPDLALF
                     FSTQRALEILGAQDGESNAPMVSPNKKGGNHGTLGVHFVLATGADYLVADVLSLGAPV
                     AMPERWFQNPPVPLDQPEVVTVNTTGLKAIHPNPFNPQTTVTFSLVSSGLVRIAIYDV
                     RGKLVRRLADTVMPAGDHSVAWNGVDDGGRTASSGVYFVRMIAGSYTETRKIVMLK"
     gene            4530..5303
                     /locus_tag="VEC56_07060"
     CDS             4530..5303
                     /locus_tag="VEC56_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HXV13950.1"
                     /translation="MPTYWEYLRIDELLSLQEPTGDEHDETLFIIIHQSYELWFKEML
                     HELGYFQTLLAKNDLPRAFHTMKRVLTILKMLVAKIDILETMTPLEFLTFRERLESGS
                     GFQSAQFRELEFVLGHKRRAMVEHFPDGSSARANLSRRWSEPTVWDAFVRFLNQNGWP
                     VPPATLERDVTAPITPSPEMQKLLIDVYKSNPSLANLCERLVDLDEGVQEWRYRHVKM
                     VERTIGTKRGTGGSEGAEYLKTTLMRPLFPDLWEIRTSL"
     gene            complement(5337..6518)
                     /locus_tag="VEC56_07065"
     CDS             complement(5337..6518)
                     /locus_tag="VEC56_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV13951.1"
                     /translation="MTNRSPALWCVLAALLLAPAVAPAGIGDSIKKKAQDKANKTADD
                     ALNKKPAEKKADETQGAGGATEAASSQEAAGDAKVSTVSTKFDFVPGDKVLLFDDFTE
                     DELGDFPAHWKLNAGNVETAEMDGERWLRLAGDYGRVRPKVEGTLPEYWTLEFDMHIV
                     EPACAGIGVEGLVEGNVTAWKAELHQYGATTVTFQSGAITSQSEYEGVPSMVGRHHVM
                     IMARQAGLKIYVDTQRVVNVPDAASVGRATEVQINFLDDDCQPMITNVRFAEGNKPTK
                     DPFANGKFVTYGIYFDSGSDVVKAESAPVLRQMAAYLEANAAVKVAVDGHTDNEGNAA
                     GNLDLSKRRAASVAKVLADQFKIAPDRFKTDGLGDTKPVASNDNAEGRAMNRRVEFTK
                     L"
     gene            6670..>7778
                     /locus_tag="VEC56_07070"
     CDS             6670..>7778
                     /locus_tag="VEC56_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HXV13952.1"
                     /translation="MISPPDLYRSPNPLAPAYSRFRVAERLLLTGHSHQAWPDAAFEG
                     QQQAFLDAAELVDDKWPRVFEVAGRVRRGWARLLDDSPECVALGHSTHELVVRWLSAL
                     PLRKRPRLVTTDGEYHSIRRLVDRLSEEGIEIHKEPASPVATLAERLARALDERTAAA
                     LVSSVLFRSAQIVPNLRALADACARAGAELLVDTYHHMNVVPFSIREMGLERAYVTGG
                     GYKYCQLGEGNAFLRFPKDCALRPVVTGWFSEFALKEKKPERPVLYGDGPARFAGATY
                     DPTSHYRAASVFDFFETRGLTPALLREVSQHQVGLLIERFDALDLDPKLIARDGSVAL
                     RDIGGFLVLQTPRAGEICQALHDRGVYTDYRAGILR"
BASE COUNT         1388 a         2545 c         2485 g         1360 t
ORIGIN      
        1 cgggacgtcg accaacatga acgcgaacga agtgatcgcc aaccgcgccg ccgaaatact
       61 cgggaaagaa cgcgggcaca agtcgatcca ccccaatgac cacgtcaaca tgagccagtc
      121 gtccaacgac gtgatcccga ccgcgatcca cgtcgcggcc gcgaccgcta ttaccaccga
      181 gctgataccg gccctcgagc acctgggtct taccctggaa gcgaaggccg agcaattcga
      241 cgacgtcgtg aagtcggggc gcacgcactt gatggacgcc accccggttc gactcgggca
      301 ggagttcggc ggcttcgagc gccaggtggc gaacgcggtc gagcgcgcgg cgcgcgcccg
      361 tgacacgctc ctcgagctgg ccttgggcgg caccgcggtg gggaccggca tcaaccggcg
      421 cgcggagttc ccgcaggtcg cgatcaagtt catcggcgaa gacacggcac tcgggttcta
      481 cgaggccatg gaccacttcg aagcgcaggg agccaaggac gcgtgcgtcg aagcctccgg
      541 ccagatgaag acgatcgccg tgagtctgtc caagatcgcg aacgacattc gactgatggc
      601 gtcgggtccg cgcaccggca tcgccgagat cacgctaccg tcgattcaac cgggcagttc
      661 catcatgccg ggcaaggtca accccgtgat gtgcgaatcg gtgacgatgg tgtgcgcaca
      721 ggtgatcggc tacgacgccg ccatcacgca aggcgggctg gggagctatc tcgagctcaa
      781 cgtgatgatg ccgctgatcg cgtacgatct tctggagcag attcgactgc tatccaatgt
      841 gagccgagtg ttcgccaaca aatgcatcgc cggtataacc gccaacgact cgcgcgcgcg
      901 cgagctgctg gaattgaatc tctccacgtg cacatcgctc gcacccaaga tcggctacga
      961 caaagcggcc gccttgtcga agcaggcctt cgcgaccggg cgcaccgtgc gcgacgtcgc
     1021 gcgcgagatg aaggtgctcc ccgaggacga gctcaatcgc ctgctggatt acaagcggat
     1081 gacggagccc gacgcgtagg cattgacgct ggccgtccgc ggtgctatca tgcgcgcaca
     1141 ttagtacttt cgaccgtcgc gggcagccag ccacaccccc tggaggtgac cgcttgacac
     1201 gtagctactc cgagtagtca ggtggtcgga agccgcatcc ttcccgctat ccccggctcc
     1261 gatcccattg gtcctaggtc catcgacgcc cccgcgttgg gctatccacg cttcttctgc
     1321 gccgcacttt ctcgcgcggt gcgtcgactc actatgtagt acaactcaaa ggaggggtct
     1381 catgagaaga ctcgcatgta tcgccgtcgc cgcgataacg attctggtga cggccggcga
     1441 cgacagggcg aaggaatcgt ttgcctcgat ggtcgtcccg caggcgccgg cggccagcgg
     1501 catggtcgtg cagatcgacc ctgccaccgg cctgcccgtc acgccgactc ccgagacgat
     1561 gctcgcgctg caggatcgga tgaagctcaa ccgctcgacc gagggactgg tcgaagtgcc
     1621 gagcgcggtc gtcggaggcg ggtacacggt cgatctccaa ggtcgcttcc agcattccat
     1681 gacggtcacc atagacgcat cgggcgtggt gcattcgacg tgcaccacct ccgggacgtc
     1741 acccgaggag gtgacggcgt catgcgtcga ccattaatga attgcctgat cgtcctggcg
     1801 gcggtgctgc tcgcggcgac gccgttcgac gcacaggccg cggccacgat cgttatccag
     1861 aacaacgacg gggcgggtga gggattcaac gatcccacgc cgttcacgcc cgtcggcggt
     1921 aacaacgccg tgacactcgg acaggcgcgg ttgatcgcct tcacgtatgc cgcgaatctc
     1981 tggggcggct gcctcaacag caacgtcacg atcacgattc gcgcgcagat ggatccgcag
     2041 acgtgcacgc cgacttccgc caccctgggt tcggccggtg cgacgacggt gcaccgcgat
     2101 ttcaccaacg cgcccgcggc caacacctgg ttctcgcagg cgctggcaaa ttcgctgaac
     2161 ggcgcggacc tcgacggcgg ccaaccggac gtcaacgcga ccttcaacag cagcatcaac
     2221 ggtaatccgg cctgcttggg cggcggatcg tggtactacg ggctcgacgg gaatcccggt
     2281 ccgaaccagt gggacttcgt caccgtggtg ctgcacgaaa tcggtcacgg cgtcgggttc
     2341 cagaccttca tcgacgacaa caccggcgcg cgattcatgg accggaacga ccagtacatg
     2401 cggttcatgg agcaagccgg tgcgacgccg tcgatgtaca cggcgatgaa cgacgcccag
     2461 cgcgcggccg gtaacatttc ggatcccaat ctgcgctgga ccggcggcag tgcgaacgcc
     2521 gagcacccga atgttcccat cacggcgggg ttgaacggcg ggctcctgcg cttgcacggg
     2581 cccaacccga cgcaacccgg ctcgtcgctg tcgcactggt cgacggcgct ctccccgagc
     2641 gagctcatgg agccgagcta cacggctccg gaccacaaca tgagcctggc gctctacctg
     2701 ctggaggaca tcgggtggtc gatcgacgac agcccgccga cgatcacctg cccctccgac
     2761 gtcgatgtcg agtgcacgac ggcgagcggc acgccacggt cgaacccggc catccaggcc
     2821 tggctggcca gcgtcacgac ctcgggggga tgcggcgaag tggttgtcac caacgacgcg
     2881 ccctcattct tcccggacgg caccaccacc gtcaccttca ccgcccgcga cggagcgatc
     2941 aacacagtga cctgcaccgc cgacgtcaac gtcgtggata cgaccccgcc ggtcatcgtg
     3001 tgtccgacgg acgtcacggt ggagtgctcg agtcattgcg gcgtggcgaa cgacgatccg
     3061 cagctcgccg cgttcttcgc gggcgtgtcg gcgaccgaca tctgcgaccc ctcaccggtc
     3121 atcacccacg atgcgccggc ctgtttcccc gagggtgaga cgacggttac cttcaccgcg
     3181 accgacgatg acttgaactt catcacctgc accgccaagg tcaccgtgga agacacgacg
     3241 ccgcccgaaa tcgacgtcgt gctgagccgc gacgtcctgt ggccgcccaa tcacaagctc
     3301 gccgaagtct gtgccgaagt gaccgtcacc gacgtctgtg accccaatcc gacgttcgtg
     3361 ctcacgagta tcgagtccga cgaaccggac aacggccagg gcgacggtaa cttccccaac
     3421 gacattcaag acgcggaata cggcaccccc gacctctgct tcgacctgcg ctcggagcgt
     3481 cagggaggag aagacggtcg caagtacacg atcatctact gtgccagcga cgaggcgaac
     3541 aacaccgcgt gcgataccgt gtgcgtgcgc gtgccgcacg atcagggcgc gggcgcgctg
     3601 tcgtcgacgg gcttcatcgt ggacggcagg gggttcgtct ccgggagcga tcagttcgct
     3661 gtcatcattc ccgcgatcga cggaatcgac gcgggagcgc tcgagacgtc ccgcgtctac
     3721 ctgggcaaca cggggggcgt ggcccagccg caagagattc gcatcgtgga agtgaattcg
     3781 gatgggcggc cggatctggc gctgttcttc tcgacacagc gtgcattgga gatcctgggt
     3841 gcgcaagacg gggagtccaa cgcccccatg gtgagtccca acaagaaggg cggcaaccac
     3901 ggcacattgg gtgtgcactt tgttttggcg acgggtgccg actacctggt ggcggacgtc
     3961 ctgtcgctgg gcgctcccgt ggccatgccc gagcgctggt tccagaatcc gccggttccg
     4021 ctcgatcagc cggaagtggt caccgtgaac acgaccgggc tcaaggcgat tcaccccaac
     4081 ccgttcaacc cgcagacgac cgtgacgttc tcgttggtga gttcggggtt ggtacggatc
     4141 gcgatctacg acgtgcgagg gaaattggta cgccgtctgg ccgacaccgt gatgcccgcg
     4201 ggcgatcaca gcgtagcgtg gaacggcgtg gacgacggcg gtcgcaccgc ctcgtcgggg
     4261 gtttacttcg tgcgcatgat cgcgggtagc tacaccgaga cgagaaagat cgtaatgctg
     4321 aagtaaaacg gagctcgttc ggtggttcgg aggcccggtg tcgagccgca aaggctcggc
     4381 gccgggcctt cattttgggc accgtgctcg gagtgaacgc cggtcgcggg ccggtccgaa
     4441 aaccaggggc acgatcgacg gaggatcgat tacaatgcgg gctggtcaat aaatctgttg
     4501 ctccgggacg cgaactccag cggggacgta tgccgaccta ttgggaatac ttgagaatcg
     4561 acgagctcct ctcgctccaa gagccgaccg gagatgagca cgacgagacg cttttcatca
     4621 tcatccacca gtcttacgag ctttggttca aggagatgct gcacgagctg ggctatttcc
     4681 agaccttact ggccaagaac gacctcccgc gcgcgttcca caccatgaag cgcgtgctca
     4741 ccatcctgaa gatgttggtg gcgaagatcg acattctcga gacgatgacg ccgctcgagt
     4801 tcctgacgtt tcgggagcga ttggagtcgg gtagcggctt ccaatcggcg cagttccgcg
     4861 agctcgaatt cgtgctgggg cacaagcgcc gcgcgatggt ggagcatttt ccggacgggt
     4921 cgtcggcgcg cgcgaatctg tcgcggcgct ggagcgagcc gacggtgtgg gatgccttcg
     4981 tgcgtttcct gaaccagaac ggttggccgg tccctcccgc cacgctcgag cgcgacgtga
     5041 ccgctcccat aacaccgtca cccgagatgc agaagctcct gatcgacgtc tacaagagca
     5101 acccctcgct cgcaaacctg tgcgagcgcc tggtggatct cgacgaaggc gtgcaggagt
     5161 ggcgatatcg ccacgtcaag atggtcgagc gcaccatcgg caccaagcgc ggcaccggag
     5221 gcagcgaggg cgccgagtat ctcaagacga cactcatgcg ccctctgttc ccggacctgt
     5281 gggagatacg gacctcactc tagctcatct tcgcgcgagc ggtgccggga tggctcctag
     5341 agtttggtga actcgacgcg gcgattcatc gcgcgccctt ccgcgttatc gttgctcgcg
     5401 acgggcttgg tatcgccgag cccgtcggtc ttgaagcgat ccggcgcgat cttgaattga
     5461 tcggccaata ccttcgcgac cgaggccgcg cggcgcttgg acagatccag gttgcccgcg
     5521 gcgtttccct cgttgtcggt gtgaccgtcg accgccacct tcaccgccgc gttcgcctcc
     5581 aggtaggccg ccatttgtcg cagcaccggc gccgactccg ccttcaccac gtcggagccg
     5641 ctgtcgaagt agattccgta ggtgacgaac tttccgttcg cgaagggatc cttcgtcggc
     5701 ttgttgcctt cggcgaagcg gacgttggtg atcatgggct ggcaatcgtc gtccaagaag
     5761 ttgatctgca cttcggtcgc gcgacccact gaagcggcat cgggcacgtt gaccacgcgc
     5821 tgggtgtcga cgtagatctt caagccggct tgacgggcca tgatcatcac gtggtgccgg
     5881 ccgaccatcg acggcacgcc ttcgtactcg gactgtgagg tgatggctcc ggattggaag
     5941 gtgacggtcg tggcgccata ttggtgcagt tcggccttcc acgcggtcac gttgccctcc
     6001 accaggccct cgaccccgat cccggcgcac gccggctcga cgatgtgcat gtcgaactcg
     6061 agcgtccagt attccgggag cgtcccttcg accttgggac gaacgcggcc gtagtcgccg
     6121 gcgagacgca gccagcgctc gccgtccatc tcggccgtct cgacgttgcc ggcattgagc
     6181 ttccaatggg cggggaaatc gccgagttcg tcttccgtga agtcgtcgaa gagaagcacc
     6241 ttgtcgccgg gcacgaagtc gaacttggtc gacacggtgg agaccttcgc gtccccggcc
     6301 gcctcctgcg aggaggccgc ctcggtcgct cccccggcgc cttgggtctc gtcggccttt
     6361 ttttcggccg gcttcttgtt gagcgcgtcg tcggccgttt tgttggcctt gtcctgtgcc
     6421 ttcttcttga tgctgtcgcc gatgccggcc ggcgcgaccg cgggcgcgag cagcagggcg
     6481 gctagaacac accaaagtgc cggggaacgg ttggtcatgg cggctccaat gggcggatgg
     6541 ttcgggtgtc gggcaccgat gcgatgactc gtcgatccat gagacggacg aaccgccccg
     6601 ccgtcacaga aaagcccggg acctcggcgt gcattcgggc gccgggtcgg ctatagtgac
     6661 ggcgtcgcca tgatctcccc ccccgaccta tatcgctccc cgaacccact ggcccccgcg
     6721 tactcgcgct ttcgggtcgc cgaacggctc ctgttgaccg gccactccca ccaagcctgg
     6781 cccgacgccg cgttcgaggg gcagcagcag gcgtttctcg acgccgccga gctggtcgac
     6841 gacaagtggc cgcgcgtgtt cgaagtcgcc ggccgggttc ggcgcggatg ggcgcggctg
     6901 ttggacgact cgcccgagtg cgtggcgctc ggtcacagca cccacgaact cgtggtgcga
     6961 tggctgtccg cactgccgct gcggaagcgc ccgcggttgg ttaccaccga cggcgagtat
     7021 cactcgatcc gacggttggt cgaccgactc tccgaagagg gaatcgagat ccacaaggaa
     7081 cccgcgtcgc ccgtcgcgac cctcgccgag cgcctcgccc gcgcgctcga cgagcgcacc
     7141 gcggccgcgc tggtgtcgtc ggtgttgttt cgaagcgcgc agatcgtgcc caacttgcgc
     7201 gcactggccg acgcgtgcgc gcgagccggc gccgagctgt tggtcgacac ctatcatcat
     7261 atgaacgtcg tgccgttctc gatccgtgag atgggactcg agcgcgcgta cgtcaccggc
     7321 ggcggataca agtactgcca actcggagaa gggaacgcgt tcttgcgctt ccccaaggac
     7381 tgcgcgctgc ggcccgtggt cacgggttgg ttcagcgagt tcgcgttgaa ggagaagaaa
     7441 cccgagcgcc ccgttctcta cggcgacgga cccgcgcgct tcgccggtgc cacctacgac
     7501 cccaccagcc actaccgcgc ggcgtcggtc ttcgatttct ttgagacgcg cgggctcaca
     7561 ccggcgctct tgcgcgaagt gagccagcac caagttggac tattaatcga gcgcttcgac
     7621 gccttggact tggacccgaa gctgatcgcg cgcgatggtt cggtggccct gcgcgacatc
     7681 ggcggcttcc tggtgctgca aacgccgcga gcgggcgaga tctgccaggc gctccacgac
     7741 cgcggtgttt atacggacta tcgggccgga atccttcg
//
LOCUS       DASPGK010000260         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_302385, whole genome shotgun sequence.
ACCESSION   DASPGK010000260 DASPGK010000000
VERSION     DASPGK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302385"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VEC56_07075"
     CDS             complement(<1..1318)
                     /locus_tag="VEC56_07075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXV13953.1"
                     /translation="MSTNRGAVRLLLVAAGAIFVVGAIVHNTWILGIAARAAGSILHR
                     PVHPPHAPLVHHAQLVYLALGAAFFVLAWLVGRWGPLDRFVRKPLVEKLALAVLVVVV
                     PSIWLEVGLRPFLPAHEKTTTLFVKDDRLGWKLRPGALDRWGDVEVSVNERGFRGPVI
                     PYPKPSGVARVVYLGDSVTFGYRVARWEDTYPFLLDALLDDSLSVETVNLAVEGYSQW
                     QQAILLADEGDRYQPDLVVIGFVLNDVTEMFYLARFGGSDEGFQLRHSYTSQWDRLLS
                     KSALVYQVQNLVREMKAKRKLGDDLRLGAIRQQALDVETLMQHPDQANVKTAWDIALA
                     DLQGIVDHCSARGVPVLVVVFPFTVQLSDPEGLSAPQRVVENYARARGIETMDLLPPL
                     VEHMRASGASADSLFLDHDHLSVEGHRVVAGILAPRVAALLAAQTTG"
     gene            complement(1319..1810)
                     /locus_tag="VEC56_07080"
     CDS             complement(1319..1810)
                     /locus_tag="VEC56_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV13954.1"
                     /translation="MSAPRIIVRDADPRDTADIVDFNRRLALETEERLLDPEVVTAGV
                     RRGFAHPELCRYFVAEIDGQVVGTTMITVELTDWRDGVLWWLQSVYVAPEARGAGVFR
                     ALYAHIAERARASKDVRGLRLYVHHTNQRAMRVYEAMGMAKADYEFYEHDWSDAVRRR
                     VED"
     gene            complement(1926..>2904)
                     /locus_tag="VEC56_07085"
     CDS             complement(1926..>2904)
                     /locus_tag="VEC56_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV13955.1"
                     /translation="LIRRVLELIDRVAESTSTSVLLLGESGVGKSLFAHTIHERSRAS
                     TGPFIEINCAALPTNLLESELFGYEPGAFTDARAQKIGLIELADGGTLFLDEITEIDP
                     VTQAKLLKFLDTKRFRRLGGDREIAVETRVIAASNRDIKDDVRVGRFREDLYYRLNVV
                     EIRVPALRERREDVESIANHYLETYKKKFGKPDLSVAPAARAMIRDYPWPGNVRELVN
                     VLERAVLLCKDAAIEPHHLPIEMPANGRKAVLRRRSDGRIEMELPTGTVTLDEVERAL
                     IEATLKRTRGNVSRAADLMGTSRGALRNKLARYKIDARSFARPPLVLQD"
BASE COUNT          501 a          949 c          998 g          456 t
ORIGIN      
        1 ccccggttgt ttgcgcggcg agtagagcag cgacgcgtgg cgccaagata cccgccacca
       61 cgcgatggcc ctccaccgac aagtggtcgt ggtcgagaaa caggctgtcc gccgaggcac
      121 cgctcgcgcg catgtgctcg accaacggcg gcaagaggtc catcgtctca atgccgcgcg
      181 cgcgcgcgta gttctcgacc acgcgctgcg gcgccgacag tccctccgga tccgagagtt
      241 gcaccgtgaa gggaaacacg accaccagca ccggcacacc gcgcgcggaa cagtggtcga
      301 cgatgccctg caagtccgcc agtgcgatgt cccacgcggt cttcacgttg gcctggtcgg
      361 ggtgctgcat gagggtttcg acgtcgagcg cctgctggcg aatcgcgccc aggcgaaggt
      421 cgtcgccgag cttgcgcttg gccttcatct cgcgcacgag attctggact tgatagacga
      481 gcgcgctctt ggacagcagg cggtcccact gggaggtgta cgaatgccgg agttggaacc
      541 cctcgtcgga gccgccgaag cgagcgagat agaacatctc ggtgacgtcg ttcagcacga
      601 agccgatgac gaccaggtcg ggttggtagc gatcgccttc gtcggccaac aagatggctt
      661 gctgccactg cgaatagcct tcgaccgcga gattcacggt ttcgacggag agcgagtcat
      721 ccaggagcgc gtccaggagg aaggggtagg tgtcctccca gcgtgccacg cggtaaccga
      781 acgtcaccga atcgccgaga tacacgacgc gcgcgacccc ggacggtttc ggatacggaa
      841 tcaccggccc gcggaatccg cgttcgttga cgctcacttc gacgtcgccc cagcggtcga
      901 gcgcgccggg ccgcaacttc cagccgaggc gatcgtcctt cacgaacaag gtcgtcgtct
      961 tctcgtgggc ggggagaaac gggcgcaatc ccacttcgag ccagatggac ggaaccacga
     1021 ccacgagcac cgcgagcgcg agtttttcga ccagcggttt gcgaacgaag cgatcgagcg
     1081 ggccccaccg gccgacgagc caggcgagga cgaagaacgc ggcgccgagg gcgagataga
     1141 cgagctgcgc gtggtgaacg agcggcgcgt ggggcgggtg gacggggcgg tgcagaatcg
     1201 aaccggccgc gcgcgccgca atcccgagga tccacgtatt atggacgatc gcaccgacga
     1261 cgaagatcgc gcccgccgcg acgagtagca atcgcacggc gccgcggttc gtcgacactc
     1321 aatcctcgac gcggcgtcgc accgcgtcgg accagtcgtg ctcgtagaac tcgtagtcgg
     1381 ccttggccat gcccatcgcc tcgtacaccc gcatcgcgcg ctggttggtg tggtggacgt
     1441 agagtcgaag cccgcgcacg tccttgctgg cgcgagcgcg ttcggcgatg tgagcgtaga
     1501 gggcgcgaaa cacgccggcg ccgcgggcct ccggcgccac gtacacgctc tgcagccacc
     1561 acagcacgcc gtcgcgccag tcggtgagct cgaccgtgat catggtggta ccgaccacct
     1621 ggccgtcgat ctcggccacg aaatagcggc acaactccgg gtgcgcgaag ccgcgccgaa
     1681 cgccggcggt caccacctcg ggatccagta gccgctcctc ggtctcgagc gccagtcgcc
     1741 ggttgaaatc gacgatgtcg gccgtatccc gaggatcggc gtcgcggaca atgatgcgtg
     1801 gagctgacat agttgcaagg cgcggcgcct gccgcccgac gaggacgtcg cgaagcgttc
     1861 ggactcggag ggcgggcagc gccgggccca tcctaccaag ctcttcgcgg gagatgcaaa
     1921 cgagatcagt cttgcaggac caggggcggg cgggcgaagg agcgcgcgtc gatcttgtag
     1981 cgcgcgagct tgttgcgaag cgcaccgcgg ctcgtgccca tcaggtcggc cgcgcgcgag
     2041 acgttgccgc gcgtgcgctt cagagtggct tcgatcagcg cgcgctcgac ttcgtcgagg
     2101 gtgaccgtcc cggtgggcag ctccatttcg atgcggccgt ccgagcggcg gcgcagcacc
     2161 gccttgcgcc cgttggcggg catctcgatg ggcaggtggt gcggctcgat ggcggcgtcc
     2221 ttgcacaaga gaacggcgcg ctcgagcacg ttgaccagtt cgcggacgtt gcccggccac
     2281 gggtagtcgc gaatcatcgc ccgcgcggcc ggcgccaccg acaagtccgg cttgccgaac
     2341 ttcttcttgt aggtctcgag atagtggttc gcgatcgact cgacgtcctc gcggcgctcg
     2401 cgcagcgccg ggacgcggat ctcgaccacg ttcaatcgat aatagaggtc ctcgcgaaag
     2461 cgcccgaccc gaacgtcgtc cttgatgtcg cggttgctgg ctgcgatgac gcgcgtctcc
     2521 accgcgatct cgcggtcgcc gcccaggcga cggaagcgct tggtgtcgag gaacttcaac
     2581 agcttggcct gcgtgacggg gtcgatctcg gtgatttcgt cgaggaagag cgtgccgccg
     2641 tcggccagtt cgatcagccc gatcttctgc gcgcgcgcgt cggtgaacgc gcccggctcg
     2701 tagccgaaca gttcgctctc gaggagattg gtggggagcg ccgcgcagtt gatctcgatg
     2761 aacggccccg tcgacgcgcg cgagcgctcg tggatggtgt gggcgaagag gctcttgccc
     2821 accccggact ctcccaggag cagcaccgac gtcgacgtgc tctcggccac gcggtcgatc
     2881 agctcgagca cgcgccggat gagc
//
LOCUS       DASPGK010000261         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_769690, whole genome shotgun sequence.
ACCESSION   DASPGK010000261 DASPGK010000000
VERSION     DASPGK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769690"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="smpB"
                     /locus_tag="VEC56_07090"
     CDS             complement(<1..442)
                     /gene="smpB"
                     /locus_tag="VEC56_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXV13956.1"
                     /translation="MKIVVQNKKARHDYAILETIEAGIALRGTEVKSLREGRANLRDS
                     YAVIEGGEVVLRGLHISPYSHTSDRTLDPLRDRKLLVNRAEIRKLVGKVNEKGMTLVA
                     LKIYFNDRGIAKIELGLGKGKRQHDKRDAIAEREVRREMDRAMKR"
     gene            879..965
                     /locus_tag="VEC56_07095"
     tRNA            879..965
                     /locus_tag="VEC56_07095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:913..915,aa:Leu,seq:cag)
     gene            1053..2189
                     /locus_tag="VEC56_07100"
     CDS             1053..2189
                     /locus_tag="VEC56_07100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV13957.1"
                     /translation="MRVLIAEASDYRGPITVGSHALAREFLRMGARVCWFGPPLDARS
                     IVSGANDPRVRRRLRAWRSDGHQDADDVFEYYPFTLLPVIDSPFLRSRFVGENTLRAT
                     LPRADSILRERGFASPDLVWLPNSRFAHAAWKLARARRSACRLSDDWAHFGRLPASLH
                     RLHDEVVDGADAVFVTSRRTAHALRGRRPDAVYLPNGVSEAFFEGAPSEPSVLARFPR
                     PRVVFVGALDAWIDFDTIAAVGERIPCASVLVFGPGEPKVRAYPANLHFLGAYPYRDL
                     PTLLRHCDVGLVPFVKSELTHAVSPLKLFEYLAAGLPTVATRLHEIEASESPATLCDD
                     LPSFVRAVESVLDGDPGGRADRVAFAREHTWTKRFEAVRATLGF"
     gene            2257..3141
                     /locus_tag="VEC56_07105"
     CDS             2257..3141
                     /locus_tag="VEC56_07105"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HXV13958.1"
                     /translation="MNRSAPLGLRLATVACTAAALFGCSPAKYSVGHVMVPVLDNARD
                     AALASDDLRTFQDGAAANLFLLEGLMRTDPDNAKLRLNASMLYFSYAFSSHEDTDPAY
                     ASQLYLKGLAHGQAALGKNKKLATDWGVPFEEFEKTLPALREKDVPAAVWTAANWAQF
                     ISLHLDSTAVLRDIPKVTALLERAAELDGSYFEGLPHVMIGSLHAFRPPMFGGSPEKS
                     RANFEQAFAIGGESFLLSKYFYARFYLYRVQEAEEFVRVLEAVGDTPSVEDDPYRLLN
                     LIARQKSAILLGETDALF"
     gene            3128..4168
                     /gene="dctP"
                     /locus_tag="VEC56_07110"
     CDS             3128..4168
                     /gene="dctP"
                     /locus_tag="VEC56_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXV13959.1"
                     /translation="MRCSSFIVAATLVALASPSHAQKAKTPAKAQVEIKLATLAPDGS
                     TWMKTMRKIDEDVRTRTENRVGFKFYPGGVQGDEKDVIRKMRNGQIHAAGFTGFGLGA
                     IVPETRVLELPFMFESLDELDYVRNATNDYYEKAFAAKEHALLGWTDVGFVYLYTKAP
                     VRKVSDMPGTKWWIWSGDQLAEIFYKAFGITPIPLAAPDVLTSLQTGVVDGVYASPLA
                     CVALQWFTRVKYMTDAPITHGISAVVASEKSLAGVSAADRAILLEVTRRELAALTQKT
                     RTQNDEAVAEIKKEGVQVVSIDAATRQEFATRGRAAWDDGVPALYSRELLERVKTLLA
                     DYRKNRTAGSSN"
     gene            4182..4760
                     /locus_tag="VEC56_07115"
     CDS             4182..4760
                     /locus_tag="VEC56_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXV13960.1"
                     /translation="MAIPSAALIVLGFVAIAVLALLRAGLERSARARRFFAFTGRIEI
                     ALIALMLLALVLFGCAQILLRNAFNSGLLWADPLMRHLVLWLGALGAALASARMRHIS
                     VDSLSRLLPPSLLPLRRLVVYGVTAVAAYALMIAAVRLIANERSYGDVAFLGIRTWVL
                     QLVLPIAFAMITYRTVLAIFLGRESAESGLEE"
     gene            4764..6026
                     /locus_tag="VEC56_07120"
     CDS             4764..6026
                     /locus_tag="VEC56_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXV13961.1"
                     /translation="MMVVVGILLAVLALYGMPLFAVFGALALILFHQSGVDVSSVAVS
                     MYSIAGSPLLVAIPLFTFAGYLLAHSKAPERLLNLSRAFFPGGLAVVALVACAVFTAF
                     TGASGVTIVAMGGLLLPALLADGYNRRFSLGLITASGSLGLLFPPSLPLIIYGYVAEV
                     EVDQLFLAGLLPGILLVIMLATYGAYRGKRDGVRRDPFSFARASAAMRAAAWEIPLPV
                     VVLGGIYGGIITVMEAAAITALYVLVVEVFVYRDVSVRRVPSIVAESMALVGGILVIV
                     MCSFGFVNYLIDAEVPMRILEWMQTYVSNKYTFLLILNVFLLIVGCLMDIFSALIVVV
                     PLIAPVAAGFGVDPVHLGIVFLTNLEIGYLTPPVGLNLFISSYRFREPVLKLASASVP
                     FLLLLFAALVVITYAPALSLVLVNAFAR"
     gene            complement(6039..>6492)
                     /locus_tag="VEC56_07125"
     CDS             complement(6039..>6492)
                     /locus_tag="VEC56_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXV13962.1"
                     /translation="VPYVVAQVLGAVVAGGVLYVIASGQSGFDVSAGFASNGYGEHSP
                     GGYTMGAALVTEIVMTMMFLIVILGATDHRAPAGLAPIAIGLCLTLIHLISIPVTNTS
                     VNPARSTGVAVFAGGWALAQLWFFWVAPVIGAVIGAVVYRSLGREAAR"
BASE COUNT          996 a         2200 c         2045 g         1251 t
ORIGIN      
        1 agcgcttcat ggcgcgatcc atctcgcgtc gtacctcgcg ctccgcgatg gcgtcgcgtt
       61 tgtcgtgctg ccgcttcccc ttgcccagac ccaactcgat tttggcaatg ccccggtcgt
      121 tgaagtagat cttgagcgcc accagggtca tgcccttttc gttcaccttg ccgaccagct
      181 tgcggatctc cgcgcggttg acgagcagct tgcggtcgcg aagcgggtcc agcgtccgat
      241 cggaggtatg cgagtaggga ctgatgtgca gcccgcgcag cacgacctcg ccgccctcga
      301 tgacggcgta cgaatcgcgc aggttggcgc gcccctcccg cagcgatttc acttcggttc
      361 cgcgcagcgc gataccggcc tcgatggtct cgaggatcgc gtagtcgtgt cgggccttct
      421 tgttctggac gacgatcttc aacggtgctc cggcagctcg gacgtggctc gattttgcag
      481 cgctcacgaa agcctagagg ggacgtcgct ccattgcaac tagcaaatat acaatcagtt
      541 agcgcgaacg ggcgccggga gccgggcccc gacgggccgc tggtggccca attgagttgt
      601 aacctctttt aattacaacg agttaagtcc tccggagccc gcccagcggc gatcgcccga
      661 gaccgctccg agcgattggg tcatcgcacg ccactgcatc ataactgtat atggtatatt
      721 gacttaacta acggcggtgg cctccgaccc gccccgctcc atcgttgact tttccaccgt
      781 cttcgactat tctgcgcgtc gaggtcgccg atggtgcttt tggctttgcc tcatgggcga
      841 tcctcttgct gccaatagcc tgctggagtt gttccgcggc cgaagtggtg gaattggtag
      901 acgcgctgcg ttcagggcgc agtgagggta acctcgtggg ggttcgagtc cccccttcgg
      961 caccatgaac tctcgatggt aggttcggac ccttcggttc cgcgacggcg gggccgaagg
     1021 gtccttcgtt ttccacggac aaggaccgcg ccgtgcgtgt gctgatcgcc gaagcgtccg
     1081 actatcgagg accgatcacg gtcgggtcgc acgcgttggc gcgcgaattc ctccgcatgg
     1141 gcgcgcgtgt gtgctggttt ggcccccccc tcgatgcccg ctccattgtg agcggcgcaa
     1201 acgatccccg cgtacgccgg cgccttcgcg cgtggcgaag cgacggccat caagacgcgg
     1261 acgacgtgtt cgagtactac ccgttcacgc tcctccctgt catcgactcg ccgttcctgc
     1321 ggtctcgctt cgtcggcgag aacaccctcc gcgcaaccct gccgcgcgcc gactcgatcc
     1381 tgcgcgagcg cggattcgct tcgccggacc tggtgtggct tcccaacagc cgtttcgccc
     1441 atgcggcctg gaagctggcg cgtgcgcgcc ggagcgcgtg ccgcctgtcc gacgattggg
     1501 cccactttgg gaggcttccc gcctcgctgc acagactcca cgacgaggtg gtcgacggcg
     1561 cggacgcggt gttcgtgacc tctcgccgca cggcgcacgc gctgcgcggg cggcggccgg
     1621 acgcggtcta tcttcccaac ggtgtttcgg aagcgttctt tgaaggcgca ccgagcgagc
     1681 cgtccgtgct cgcgcgcttt ccgcgaccgc gtgtggtctt cgtcggagcg ctcgacgcgt
     1741 ggatcgactt cgataccatc gcggccgtgg gcgagcgaat cccgtgcgcg tccgtgctcg
     1801 tcttcgggcc cggggagccc aaggtccgcg cctatccggc caacctccat ttccttggcg
     1861 cctatccgta ccgcgatctg cccacgctgc ttcgacattg cgacgtcggt ttggttccgt
     1921 tcgtcaagag cgagctcacg catgcggtga gcccgctcaa gctattcgag tatctcgctg
     1981 ccggactgcc gaccgttgcg acccgactgc acgagatcga agccagcgag tcgccggcca
     2041 cactctgcga cgaccttccg tcattcgtcc gtgcggtcga gtcggtgctg gacggcgatc
     2101 ccggcggccg cgccgatcgc gtcgcgttcg cccgcgagca cacctggacg aagcgcttcg
     2161 aagccgtgcg cgccacgctg ggattctgac cgcgagtacg cccccgcccc gaaatccgag
     2221 ttgatcgccg cgccggattc gggagagaat gcccgcgtga atcgttccgc acccctcggc
     2281 ctccgtctcg ccaccgtcgc gtgcaccgcc gccgcgctct tcggctgctc gcccgcgaag
     2341 tattcggtcg gtcacgtcat ggtgcccgta ctcgacaacg cccgcgacgc cgcgctggct
     2401 tccgacgacc tgcgcacatt ccaggacggt gccgctgcca atctgttcct gctcgagggg
     2461 ctcatgcgca ccgaccccga caacgcgaag ctgcgtctga atgcctccat gctctacttc
     2521 tcctatgcgt tctcgtcgca cgaggacacc gaccccgcct acgcgtcgca gctctatctc
     2581 aagggattgg cgcacggcca ggctgcactc ggaaaaaaca agaagctcgc gaccgactgg
     2641 ggcgtcccgt tcgaggaatt cgagaagacg ctgccggcgc tgcgcgagaa ggacgtcccc
     2701 gccgcggtat ggaccgcggc gaattgggcg cagttcatca gcctccacct cgactcgacg
     2761 gcggtgctgc gcgatattcc caaggtgacg gctctgctcg aacgcgccgc ggaactcgac
     2821 ggctcctact tcgaagggct cccgcacgtg atgatcggga gcctgcacgc gttccgcccg
     2881 cccatgttcg gcggcagccc cgagaagtcg cgggccaact tcgagcaggc gttcgcgatc
     2941 gggggcgagt cgttcttgct gtcgaagtat ttctacgcgc gcttctactt gtaccgcgta
     3001 caggaagccg aagagtttgt gcgcgtcctg gaggccgtgg gcgacacgcc gtccgtcgag
     3061 gacgatccct atcgcctgtt gaacctcatc gcacgacaga agtctgccat cttgctagga
     3121 gagaccgatg cgctgttcta gtttcatcgt cgcggcgacg ctcgtcgccc tcgcttcgcc
     3181 gtcgcacgcc cagaaggcca agactcccgc caaggcgcag gtcgagatca agctcgccac
     3241 gctggcaccc gacggcagca cgtggatgaa aacgatgcgc aagatcgacg aggacgtgcg
     3301 cacccgcacg gagaaccgcg tgggcttcaa gttctacccc ggcggcgtgc agggcgacga
     3361 gaaagacgtc attcgcaaga tgcgcaatgg gcagatccac gccgccggat tcaccggatt
     3421 cggcctgggc gcaatcgtcc cggagacgcg cgtgctcgag ctgcccttca tgttcgagtc
     3481 gctcgacgag ctcgactacg tgcgcaacgc caccaacgac tactacgaga aggcgttcgc
     3541 ggccaaggag cacgcgctcc tgggctggac cgacgtcggc ttcgtgtacc tgtacacgaa
     3601 ggcgccggtg cgtaaagtgt ccgacatgcc gggaacgaag tggtggatct ggtcgggcga
     3661 ccaactcgcc gagatcttct acaaggcgtt cggcatcaca cccattccgc tggcggcgcc
     3721 cgacgtgctg acctcgctcc agacgggcgt cgtggacggc gtgtacgcgt cgcccctcgc
     3781 gtgcgtggcg ctgcagtggt tcacgcgggt caagtacatg accgacgccc ccatcacgca
     3841 cgggattagc gccgtcgtcg ccagcgagaa gtccctcgcg ggagtgagcg ccgcggaccg
     3901 cgcgatcctg ctcgaggtga cgcgccggga gctcgccgcc ctaacccaga agactcgcac
     3961 gcagaacgac gaggccgtcg ccgagatcaa gaaggaaggg gtgcaggtgg tttcgatcga
     4021 cgccgccacc cggcaggagt tcgccacgcg cgggcgcgcg gcctgggatg acggcgtgcc
     4081 cgcgctctat tcgcgcgagc tcttggagcg cgtgaaaaca ctcctcgccg attaccggaa
     4141 gaaccggact gccggctcgt cgaattgatc gccgccgtgc catggcgatt ccatcggcag
     4201 cgctgatcgt cctgggcttc gtcgcgatcg ccgtcctggc cctgctgcgg gcgggcctgg
     4261 agcggtccgc gcgcgcgcga cgatttttcg cgttcaccgg ccgaatcgag atcgccctca
     4321 tcgcgctgat gctcctggcg ctcgtgctgt tcggctgcgc ccagatcctg ttgcgcaacg
     4381 cgttcaactc cgggctgctg tgggcggacc cgctgatgcg tcacctggtc ttatggctgg
     4441 gggcgctggg cgccgcactc gcctcggcgc gtatgcgcca catctcggtc gactcgcttt
     4501 cgcgcctgct cccgccctcg ctcctgccgc tgcggcgctt ggtcgtgtac ggagtcaccg
     4561 cggtggcggc ctacgcgctc atgatcgccg ccgtccgcct gatcgcgaac gagcggagtt
     4621 acggcgacgt ggctttcctc ggcatccgca cgtgggtgct gcaactggtg ctgccgatcg
     4681 cgttcgcgat gatcacctat cgcaccgtgc tcgcgatctt tctcggccgc gaatcggcgg
     4741 aatccggcct ggaggaataa ccgatgatgg tcgtcgtcgg cattctcctc gccgtgctgg
     4801 ccctgtacgg gatgcccctg ttcgcggtct tcggcgcact ggcgctgatc ctgttccacc
     4861 aatcgggggt cgacgtctcc tcggtagcgg tctcgatgta ctcgatcgcc ggctcgccgc
     4921 tgctcgtcgc catcccgctc ttcacgttcg cggggtacct gctcgcacac agcaaggcac
     4981 ccgaacggct cttgaacctg tcgcgcgcgt tcttccccgg cggcctcgcg gtggtggcac
     5041 tggtcgcgtg cgcggtcttt accgcgttca cgggcgcgtc cggcgtcacc atcgtcgcga
     5101 tgggagggct cctgctcccc gcgttgctcg cggacggata caaccggcgt ttcagcctgg
     5161 ggctgatcac cgcgtccgga agcctcggac tgctctttcc gcccagcttg ccgctcatca
     5221 tctacggcta cgtggccgaa gtcgaagtcg accagttgtt cttggccggc ctgctacccg
     5281 gaatcctgct cgtgatcatg ctggcgacct acggcgcgta ccgcggcaaa cgcgacggcg
     5341 tgcggcgcga tccgttttcg ttcgcgcgcg cgagcgccgc gatgcgcgcg gcggcgtggg
     5401 agatcccgct ccccgtggtg gtactgggcg gcatctacgg cggcattatc acggtgatgg
     5461 aagcggcggc gatcacggcg ctctacgtgc tggtcgtgga ggtattcgtg tatcgcgacg
     5521 tctctgtgcg gcgcgttccg tcaatcgtcg cggagagcat ggcactggtg ggtgggatcc
     5581 tcgtgatcgt gatgtgctcg ttcggcttcg tgaactactt gatcgatgcc gaagtcccca
     5641 tgcgaatcct ggagtggatg cagacctacg tgtccaacaa gtacacgttc ctactgattc
     5701 tgaacgtttt cttgctcatc gttgggtgcc tgatggacat cttttcggcg ctcatcgtgg
     5761 tggtcccgct gattgcgccg gttgcggcgg ggttcggcgt cgaccccgtg catttgggta
     5821 tcgtgtttct caccaacctc gagatcggct atctgacgcc tccggtgggc ctcaatctgt
     5881 tcatttcgtc gtaccgcttc cgcgagcccg tgctaaaact cgcgagcgcc agcgttccgt
     5941 ttctcctctt gttgttcgcg gctctggtcg tgatcacgta tgcgccggca ctgagtctcg
     6001 tgctggtgaa cgcgttcgca cgctgaaacg accacgccct agcgcgcggc ctctctgcca
     6061 agcgagcgat acacgacggc gccgatcacc gccccgatca cgggagcgac ccagaaaaac
     6121 cacagctgcg caagcgccca accgcccgca aacaccgcaa cacccgtgct gcgcgccgga
     6181 ttcaccgacg tattggtgac cggaatgctg atcaaatgga tcagcgtcaa gcacaagccg
     6241 atcgcgatcg gcgcgagccc ggccggcgcc cgatggtcgg tggctccgag gatgacgatg
     6301 aggaacatca tggtcatgac gatctcggtg acgagcgcgg cacccatcgt gtagccgccg
     6361 ggcgagtgct cgccgtaacc gttggaagca aaccccgcgg acacgtcgaa gccactctga
     6421 ccgctcgcaa tcacgtacag caccccgccg gccacgaccg cgccgagcac ctgggccacg
     6481 acgtacggca cg
//
LOCUS       DASPGK010000262        13212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_769702, whole genome shotgun sequence.
ACCESSION   DASPGK010000262 DASPGK010000000
VERSION     DASPGK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769702"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VEC56_07130"
     CDS             complement(<1..799)
                     /locus_tag="VEC56_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13963.1"
                     /translation="MRTPKVTIALVFSMVLATAFASVAVADEDPPGTHRDWGIGAHIR
                     AGGAGLSYGDFLEKGTAWDFELFRQRGDWRYGVGLTFGSLAMKSPYEHEPEWAHFETF
                     ISASRVFRNGESVRPYLQGRFAVARSHPRSTLFAKNDIDSLESGESPTDAHNGVGLSL
                     VPGMEFEVAHGVAIDVAAYLNWWLTSTYPLQDYQGNRPLGSQEDPNNGLEWGGRIGLT
                     WRPMSYEEIVRPSAAPDLTPLPEMDRHKDAWGVTRSYGWAYGEVLAIN"
     gene            complement(843..1895)
                     /locus_tag="VEC56_07135"
     CDS             complement(843..1895)
                     /locus_tag="VEC56_07135"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HXV13964.1"
                     /translation="MKRVLVAAFGCVMGVSSAAWPQSHTTAPVLKYPISARSAGMGET
                     GVADNSDPANIYYNPANVVGPARVYVQGSRWDIAPLIADDLWIGGGSAGVSYARRDAN
                     PFVWSADVSYERFDYGTSIATTPSGQPLGEYETVEHCIALTIGAGLSIGERSELRVGA
                     AAKRWDPDYAPDEFTIPDAPEDVTGYAFDAGATFALRAVVAEWNVTPAVALAIVNAGP
                     DVEYDSDNEDPLPTRFHFGTSVRVESPTVHVLTASVPLIALVYNLEGVERFHDDGFSW
                     GIGGELAVAQIVFVRAGVSDFDDEDTIADDYKDESGWGFGLGVPAGSLRARFDYAKTS
                     DAFDEEKLGFELAWLL"
     gene            complement(1899..4169)
                     /locus_tag="VEC56_07140"
     CDS             complement(1899..4169)
                     /locus_tag="VEC56_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HXV13965.1"
                     /translation="MSIAPRIPVRNRACAACLTAVAALLVASASLAGDKEWPQAVDVK
                     EGKIVMYQPQPETLEGNVLKSRAAVSILPKGTTEPVFGAVWLESQVEVKKDDREVKIL
                     GTRVINARIADSKPGQEERFMEILQEEIGDSEFKIDYDRLIASLQDAEVQATTAEGLR
                     NDPPKIIIEYAPAILVVIDGQPTLRAIPDSDLERVVNTPFFIVKKDDRYWLNGGEDWF
                     STKEFTGEWERDRVPVSVNDAWKDDLKRQGTTPAKAEESKDNREPKIIAATEPTELIV
                     IDGEAQYRPIVGDELLYVSNSESDVFMEVASQNYFVLLSGRWYRSKSMDGPWQYVQSD
                     ALPECFVRIPTNSDKSQVRVFVAGTQEAKEAVLDSQVPQTAAINRAAATLTVTYDGEP
                     KFEPIPKTSIELAQNTDKTVMKIDGRYYCCENAVWFIADAPKGPWAVCDRVPDEVQKI
                     PPESAAYNTKYVYIYESTPEVVYVGYTPAYVGCYPYYGSIVYGTGWYYPPYVSPHYYY
                     PRPCTWGFHVNYNPWTGWSCGMSWSNGWAHFSVGFGGGGGWWGPPGYAWRPNYPAHHG
                     NVIVNRPVNINTGNRVSHHNGQDNLYQRPSTRPAVADRETAARNRPGSSPGVSTREAK
                     PSRSRENNVYADQNGNVMRRENDGSWSQRDQGSWKPADGRPQSKDAATRPGGGASAGQ
                     QPATRPGGATPGTQPATRPATPQTRPSQPNTQQLNRDYQSRQRGNQRVNNYQGQTRSR
                     PSSRPAGRSGGGGRRR"
     gene            4331..5023
                     /locus_tag="VEC56_07145"
     CDS             4331..5023
                     /locus_tag="VEC56_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13966.1"
                     /translation="MRISIHPASWRTLACVLLLSMALDSCSSSQLVNMWKDPAHPAQP
                     IDKMYVIGFRTDQARRRFIEDAVVAELTRVKVSAVPSYRDFATALPDTQQVVDVVSRD
                     GYNGVIIVMPLGTETRETYVPGYVTTMPTSYYGGYGGYGGYGGYGYGGHGYWGGYYYP
                     AYQTVYEPGYVEVDQIVRIRIDIWSAENGGMLVWSGTTESVDPTSAEQIRKEVLQLVV
                     PELRRSGVIANR"
     gene            complement(5007..5903)
                     /locus_tag="VEC56_07150"
     CDS             complement(5007..5903)
                     /locus_tag="VEC56_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13967.1"
                     /translation="MRRVRSSGRAHLSAITLAALVLGGFAAWGLLHHMRKPELTPELR
                     GYALAEELGCHGCHGPRGTGGVRNPRAGFQQTTGEIPAWDGGSAMMYVKSEDEIREWI
                     LDGRPWRLVLRDSLAARAARADSDTTSPFPEPGRVELRPGRLATVTPPVRMPAYRGVV
                     DDQQLEDLVAYYKAVAGYGSEVDAMPADARAGYAIARDTGCFGCHGPGGLIGVKNPRS
                     FKGYIPPWHGPDFHDLVRNDDELRAWILDGGIARIAENRVGRWFTTRQVVKMPAYSGV
                     LADSSVADLMTYIKWTAATYSR"
     gene            complement(6049..6849)
                     /locus_tag="VEC56_07155"
     CDS             complement(6049..6849)
                     /locus_tag="VEC56_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13968.1"
                     /translation="MAASILAALAGCGEDRTSNADDRKPIAVTASGIEIMEVKRNEPE
                     RIVEAVVVAGGEERQVTLAPVLDGPLPFAVTATIHPAGGRGPVALSYGWDQHSGAAWF
                     QQSSDGETFEMSRVTTQGRVVEEYDFDGHFLRLEYAELPAAVVDKTIEKFHRGESLDA
                     SSPDVIELGGAIREFEDFVAQASPYLEAEGEDVELLTSLLGDRVFVEAVVGEDIEPAR
                     PDAFCRFMNLCAALSCRFIVNPHVCALCTAGSLACLFMDAFCAAWCGG"
     gene            complement(7181..7567)
                     /locus_tag="VEC56_07160"
     CDS             complement(7181..7567)
                     /locus_tag="VEC56_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13969.1"
                     /translation="MKKTLFVAMAIVLATSAIALAGDDAKKAKAAPTTLTGEVLDLYC
                     YMGHPESATGAEHAKCAQACVSKGLPIGFLTADGTIYLIIGKDHESASTAVAEWVGKQ
                     STITGHIKEQKGMKAIELVSIAAAKS"
     gene            complement(7668..9782)
                     /locus_tag="VEC56_07165"
     CDS             complement(7668..9782)
                     /locus_tag="VEC56_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HXV13970.1"
                     /translation="MATMYTCPMHPEVRQSGPGSCPKCGMALEPEIPTAGSEPETNPE
                     LKDMTRRFWVSVALSIPIVLLAMGHMVFTGAHLVSPRARTFIELALATPVCVWAAWPF
                     YARAVASVRNRSLNMFTLIGLGVGVAYVYSVVAALVPQIFPASFREGGVVAVYFEAAA
                     VIVTLVLLGQVLELRARSRTGSAIKKLLGLQPATARRLCDHGHEHDIPLSHVHVGDRL
                     RVRPGEKVPVDGVVLEGTSAVDESMVTGESMPVEKQPGDRVIGSTVNASGSFIMRAEK
                     VGSETLLARIVAMVAEAQRSRAPIQKLADMVSGYFVPIVIVVAVITFAVWALAGPEPR
                     MAHAIVNAVAVLIIACPCALGLATPMSIMVATGKGATIGVLFRNAEAIETLRKVDTLV
                     VDKTGTLTEGRPKLVAVEARGVDERELLHVAASLERGSEHPLAAAVVKGAHERGVSPA
                     TAEAFISIAGQGVRGRVNGREVALGNRALMESIGARLDDLAERAESMRASGQTVMFVA
                     VDGRIAGLLGVADPIKPTTPDAIRKLNDDGVRVVMLTGDSATTAHAVARQLGIGDVMA
                     EVLPDQKASIVKRLQSEGHVVAMAGDGVNDAPALAQADVGIAMGTGTDVAMESAGVTL
                     VKGDLRGIARARRLSRETIANIKQNLFFAFVYNAAGVPLAAGVLYPAFGLLLSPIVAA
                     AAMSFSSVSVIGNALRLRKGEV"
     gene            complement(9794..13117)
                     /locus_tag="VEC56_07170"
     CDS             complement(9794..13117)
                     /locus_tag="VEC56_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXV13971.1"
                     /translation="MIHKWIAFSAANRVLVIMLTAAALVYAFYTMRHIRLDAIPDLSD
                     TQVIVYTKWDRSPDIMEDQVTYPIITALLGAPKVKTIRGFSDFGFSYVYVIFQDGTDI
                     YWARSRVVEYLQKITGTLPPGVNPSLGPDATGVGWVYQYALVDKTGKHSLADLRSFQD
                     WNLRFALQSVAGVAEVAGIGGFQKQYQITIDPNRLQSYGLGMMEIIEAVRKSNNEVGG
                     RLIEWSGREYMVRARGYIQDTAALEDVVVKTSTQGTAVLLRDVATIALGPQLRRGVAD
                     FDGDGDAVGGIIVMRHGENALNVIERVKSKLEELKPSLPEGVEVVSTYDRSHLIKESI
                     DNLKHELIVEMIIVSLVIMFFLFHGPSAFVPIITIPVTVALSFIPMYHMGVTSNIMSL
                     AGIAISVGVLVDGAIVLVENAYKKLEIWHEQGRVGDYHDTLLKAIQEIAPSVFFSLLV
                     IAVSFLPVFTLIDQEGRLFKPLAYTKTLAIALAALLAVTLDPAVRMMFTRMDPIRARP
                     RWLGRALTTALVGRYYPEEKHPISRILFRLYEKPCRLVLRHPRKVIALSALVVATTIP
                     VYMKLGHEFMPPLNEGAILYMPTTLPGLSVTEAQRLMQIEDRILKGFPEVVTVWGKAG
                     RADTSTDPAPFSMMETTVVLKPKEQWRHKERFFSGWPKPLREVLGHVWPEHISWDELI
                     DEMDRALQIPGNVNAWTMPIKGRIDMLSTGVRTPIGIKIFGADLDQLQRIGEQVEGIV
                     RQVPGTRSVYAERAGGGYFVDFVPKRRELARYGLTIDDLHAVIMSAVGGENITTTVEG
                     RERYSVNLRYPRDLRADLDQLRRVLVTARVGGMRERAGDGMGSRGSDAGAAEPIQVPM
                     SELADIELVNGPSMIRDENGMLSAYVYVDITGRDIGGYVDEAKRAVAGQLQVPTGYSL
                     VWSGQYENMLRVRERLKIVIPVTILLIFLLLYANTKSTMKAMIVMLAVPFSLVGAVWF
                     LYLLDYNVSIAVWVGMIALAGLDAETGVFMLLFLDMSHDEYKRAGKLKTVADLHEAIV
                     HGAVKRIRPKMMTVMAAGMGLMPIMWSTGTGADMMKRVAAPMVGGLFTSFGMELLVYP
                     AIYLIWRSRELKSAAPSSA"
BASE COUNT         2194 a         4454 c         4215 g         2349 t
ORIGIN      
        1 aattgatcgc gagcacttcg ccgtaggccc agccgtagct gcgcgtcaca ccccacgcgt
       61 ccttatggcg atccatctcc ggaagcggcg tcaagtcggg cgccgcactc ggacgcacga
      121 tttcctcata cgacatcggc cgccaggtca atccgatgcg gccgccccat tcgagtccgt
      181 tgttggggtc ttcctgcgat cccagcggac gattgccctg gtagtcctgc agcgggtacg
      241 tcgacgtgag ccaccaattg aggtacgcgg cgacgtcgat cgcgacgccg tgcgcgacct
      301 cgaactccat ccccgggacg aggctcaaac cgactccgtt gtgcgcgtcg gtcgggctct
      361 cgccggactc gagcgagtca atgtcgttct tcgcgaacaa cgtcgagcgt gggtgcgagc
      421 gcgcgaccgc gaagcgtccc tgcaggtacg gccgcaccga ttcgccgttg cgaaacacgc
      481 gcgaggcgga gatgaacgtc tcgaagtgcg cccactcggg ctcgtgctca taaggcgact
      541 tcatcgcgag cgaaccaaag gtcaagccga cgccgtagcg ccagtcgccg cgctgccgga
      601 agagctcgaa gtcccacgcc gtccccttct cgagaaaatc accgtagctc aagcccgcgc
      661 cgccggcgcg aatgtgcgcg ccgatacccc aatcgcggtg cgtgccggga ggatcctcgt
      721 ctgcgacggc gaccgaagcg aatgcggtcg ccaacaccat cgagaagacc agtgcgatcg
      781 tgaccttagg ggttcgcatc tcgacctctt tcattctgaa cggagcctcg gaccggtgcg
      841 cattatagaa gccacgccaa ctcgaatccc aacttttcct cgtcgaaggc gtcggacgtc
      901 ttcgcgtagt cgaagcgcgc gcgaagcgag ccggccggca cgccgagccc gaagccccag
      961 ccggattcgt ctttgtagtc gtcggcgatc gtgtcttcgt cgtcgaagtc cgaaacaccc
     1021 gcgcgaacga acacgatctg cgcgacggcc aattcgccgc cgattcccca cgagaaaccg
     1081 tcgtcgtgga atcgctccac gccttcgagg ttgtagacca gtgcgatgag cggcacgctc
     1141 gccgtcagca cgtggacggt cgggctttcg acgcgaacgc tggtgccgaa atggaatcga
     1201 gtggggagcg ggtcttcgtt gtccgagtcg tactccacgt cgggacccgc gttgacgatc
     1261 gcgagtgcga ccgcgggcgt cacgttccat tcggccacga cggcgcggag tgcgaacgtg
     1321 gctccggcat cgaacgcgta gcccgtgacg tcctcgggcg catccggaat ggtgaactcg
     1381 tcgggcgcat agtcggggtc ccatcgcttg gcggctgcgc ccacgcgcaa ttccgaccgc
     1441 tctccgatcg agaggccggc gccaatggtg agcgcgatgc agtgctcgac ggtttcatac
     1501 tcgccgaggg gctggccgct cggcgtggtc gcgatgctcg tcccgtagtc gaagcgttcg
     1561 taggagacgt cggcgctcca cacgaacgga ttcgcgtcgc gtcgcgcata ggacacgccg
     1621 gcgctcccgc cgccgatcca caagtcgtcg gcgatcaggg gtgcgatgtc ccagcgcgag
     1681 ccctgtacat acacgcgcgc gggtcccacg acgttcgcgg ggttgtagta gatgttcgcg
     1741 gggtcggagt tgtcggcgac acccgtctca cccatccctg ccgagcgcgc gctgatgggg
     1801 tacttgagca cgggggccgt ggtgtgtgac tgcggccatg ccgccgatga cacccccatc
     1861 acgcacccaa acgccgccac gaggacccgc ttcattttct atcgccgccg tccaccgcca
     1921 ccggatcgcc cggccggtcg tgacgacggc cgcgagcgcg tctggccctg gtagttgttg
     1981 acgcgctggt tgccgcgctg gcgactctgg tagtcacggt tgagctgctg cgtgttcggc
     2041 tgcgacgggc gcgtctgcgg cgtcgccggc cgtgtcgcgg gttgcgtgcc cggggttgcg
     2101 ccgccgggtc gcgtcgctgg ctgctggccc gcgcttgcgc cgccaccggg gcgcgtggcc
     2161 gcgtccttgc tctgcggacg cccgtcggcg ggcttccacg agccctggtc gcgctgcgac
     2221 caggagccgt cgttctcgcg ccgcatcacg ttgccgttct gatcggcgta cacgttgttc
     2281 tcgcgcgagc gcgacggctt cgcttcgcgc gtgctcacac ccgggctcga tccggggcga
     2341 ttgcgcgcag cggtctcgcg gtcggcgacc gcgggcctgg tcgacgggcg ctgatacaag
     2401 ttgtcctggc cgttatggtg actgacccgg ttccccgtgt tgatattgac ggggcgattc
     2461 acgatcacgt tgccgtggtg cgcgggatag ttcggccgcc acgcgtagcc cggaggtccc
     2521 caccacccgc cgcctccgcc gaaaccgacc gagaagtgcg cccacccgtt gctccagctc
     2581 atcccgcagc tccaccccgt ccacgggttg tagttcacgt ggaatcccca cgtgcacggt
     2641 cgcgggtagt agtagtgcgg gctcacgtag ggcgggtaat accaacccgt gccgtacacg
     2701 atcgagccgt agtacggata gcagcccacg tacgcgggcg tgtagccgac gtacacgacc
     2761 tccggcgtcg attcgtagat atagacgtac ttcgtgttgt aggcggcact ctcgggcgga
     2821 atcttctgca cttcgtcggg caccctgtcg cacaccgccc acggtccctt gggcgcgtcg
     2881 gcgatgaacc acaccgcgtt ctcgcagcag tagtagcgtc cgtcgatctt cattacggtt
     2941 ttgtcggtgt tctgcgccag ctcgatcgac gtctttggga tgggctcgaa cttgggctcg
     3001 ccgtcgtagg tcaccgtgag cgtggccgcg gcgcgattga tggcggcggt ctggggaacc
     3061 tgcgagtcga gcacggcctc cttcgcctct tgcgtgccgg cgacgaacac gcgtacctgg
     3121 ctcttgtccg aattggtcgg gatgcgcacg aagcactcgg gcagcgcgtc cgactgcacg
     3181 tactgccacg gcccgtccat cgacttgctc cggtaccagc gtcccgacaa gaggacgaag
     3241 tagttctgcg acgccacttc catgaacacg tcgctctcgc tgttgctcac gtagagcagc
     3301 tcgtctccca cgatggggcg gtattgcgcc tcgccgtcga tgacgatgag ctcggtgggc
     3361 tcggtggcgg cgatgatctt cggctcgcgg ttgtctttcg attcctcggc cttggcgggt
     3421 gtggtgccct ggcgcttgag gtcgtccttc cacgcgtcgt tcaccgagac gggcacgcgg
     3481 tcgcgctccc actcgccggt gaactccttg gtcgagaacc agtcctcgcc cccgttgagc
     3541 caataccggt cgtccttctt cacgatgaag aagggcgtgt tgaccacgcg ctcgagatcg
     3601 gagtcgggga tggcgcgcag ggtcggctgg ccgtcgatga cgaccaggat cgcgggcgcg
     3661 tactcgatga tgatcttcgg cgggtcgttg cgaagtccct cggccgtggt cgcctggacc
     3721 tcggcgtcct gcaggctcgc gatcaggcgg tcgtagtcga tcttgaactc ggagtccccg
     3781 atctcctctt gcaggatctc catgaagcgc tcttcctggc cgggcttgga gtccgcgatg
     3841 cgcgcgttga tgacgcgcgt gccgagaatc ttcacttcgc ggtcgtcctt tttgacctcg
     3901 acttgactct cgagccacac cgcgccgaac acgggctcgg tggtcccctt tggcaaaatc
     3961 gacaccgccg cgcgcgattt gagcacattg ccttcgagcg tctcgggctg cggctggtac
     4021 atcacgatct tgccctcttt gacgtcgacc gcctgcggcc actccttgtc gccggcgagc
     4081 gacgccgatg caacgaggag cgccgcgacc gcggtcagac atgcggcgca ggcgcgattg
     4141 cgaacgggta tgcgtggtgc gatcgacata gtgccttcct cgagttggaa cgcgtgagcg
     4201 ggcccaagcg taaacgagag agagcgccgc cgcaagcgca tagcgcgcct tgagaaccgg
     4261 cggttcgcct ggtagaatcc ggctgtcccc tgccgccgtt gcgcactcga accaaggagc
     4321 ccatttcgtc atgcgtatct cgattcatcc cgcctcgtgg cgcaccctcg cctgcgtgct
     4381 cctgctctcg atggcgctcg actcttgttc gagctcgcag ctggtcaaca tgtggaagga
     4441 ccccgcgcat cccgcgcagc ccatcgacaa gatgtacgtc atcggctttc gcaccgacca
     4501 agcgcgccgc cgatttatcg aggacgcggt ggtggccgag ctgacgcggg tcaaggtgag
     4561 cgcggttccg tcgtaccgcg atttcgcgac cgcgctcccg gacacgcagc aggtcgtcga
     4621 cgtcgtctcg cgcgacggct acaacggcgt catcatcgtg atgcccctgg gaaccgagac
     4681 gcgcgagacg tacgtgcccg gctacgtaac gacgatgccc acgtcgtact acggaggcta
     4741 tggaggctac ggcggctacg gcgggtacgg gtacggcggg cacggctact ggggaggtta
     4801 ctactatccc gcgtaccaga ccgtctacga gcccggctat gtcgaagtcg atcagatcgt
     4861 gcgcattcgc atcgacatct ggagcgccga gaacggcggg atgctggtgt ggtccggaac
     4921 cacggagtcg gtcgatccca ccagcgccga gcagattcgg aaggaagtgt tgcagctggt
     4981 cgtgccggag cttcgtcgaa gcggcgtcat cgcgaatagg tagcggccgt ccacttgatg
     5041 taggtcatga ggtcggcgac gctcgaatcg gcgagcacgc cactgtacgc gggcatcttg
     5101 acgacttggc gcgtcgtgaa ccaccgcccg acccggtttt cggcgatgcg ggcgataccc
     5161 ccgtcgagaa tccaggcgcg cagctcgtcg tcgttgcgta ccagatcgtg gaaatcggga
     5221 ccgtgccagg gcgggatgta gcccttgaag ctgcgcggat tcttgactcc gatgagcccg
     5281 cccggcccgt ggcagccgaa gcaccccgtg tcgcgcgcga tcgcgtatcc ggcgcgcgcg
     5341 tcggccggca tcgcatccac ctcgctgcca taccccgcga cggccttgta ataggcgacg
     5401 agatcctcga gctgctgatc gtcgacgact ccccgatagg cgggcatgcg aaccggcggc
     5461 gtcaccgtcg cgaggcggcc cggccgcagc tcgacgcgcc ccggctccgg aaacggcgac
     5521 gtggtgtcgg agtccgcgcg cgccgcgcgc gccgccagcg agtcgcgcaa caccaaccgc
     5581 cacggtcgac cgtcgaggat ccactcgcga atctcgtctt cgcttttcac gtacatcatc
     5641 gcgctgccgc cgtcccacgc cgggatctcg cccgtggtct gctggaagcc cgcgcgcggg
     5701 ttgcgaacgc cgccggtgcc gcgcggcccg tggcaaccgt ggcaaccgag ttcctcggcg
     5761 agcgcatagc cgcgaagctc gggcgtgagc tcgggctttc gcatatgatg gaggagtccc
     5821 cacgcggcga aaccgccgag cacgagcgcg gcgagcgtga tggcggagag atgggcgcga
     5881 ccggaggacc gcacgcggcg cattatatcc gcgaccgctc caaagtgcga acgtggaatt
     5941 gtcggagtcg ccgagcgcgc ggcgggacga cggccccggc gcaaactacc gccggagccg
     6001 tcgcgagcat gggcctcccc tgtcgagctt gtgggcgcgc gcctgcgact atccgccgca
     6061 ccacgccgcg cagaacgcat ccatgaagag acacgccagc gatcccgcgg tgcacagcgc
     6121 gcacacgtgc ggattgacga tgaaccggca cgagagcgcc gcgcacaagt tcatgaaacg
     6181 acagaaggca tccgggcgag ccggctcgat gtcctccccg acgacggcct ccacgaagac
     6241 gcggtcgccc agtagggacg tcaggagctc cacgtcttcc ccttccgctt cgagatacgg
     6301 cgacgcctgg gcgacgaagt cctcgaactc gcgaatcgct cccccgagct cgatcacatc
     6361 cggcgacgac gcgtcgaggg actccccgcg gtggaacttt tcgatcgtct tgtcgacgac
     6421 ggccgcgggc agctcggcgt actccaaccg aaggaagtgg ccgtcgaagt cgtactcctc
     6481 gaccacgcgc ccctgtgtcg tcacgcgcga catctcgaac gtctctccgt cgctgctttg
     6541 ttggaaccac gccgcgccgg agtgctggtc ccaaccgtac gacaaggcca ctggccctcg
     6601 accacccgcc ggatgaatcg tcgcggtcac ggcgaaggga agcggaccgt cgaggaccgg
     6661 cgcgagcgtc acctgtctct cctctccccc ggcgacgacg accgcctcga cgatccgctc
     6721 tggttcgttc cgtttgacct ccatgatttc gatgcccgag gccgtgacgg cgatgggctt
     6781 ccggtcgtcg gcattgctgg tgcgatcctc cccgcatccc gcgagtgccg ccagaatcga
     6841 cgccgccacg atgccattcg tccaacgacg cgcattcgtg cgtcgtaccc caaccactag
     6901 actcgtcatg actcaacctc ccgttcatcc accccacccg gcacaaccag tcacgcggac
     6961 ggtataggca cggagtgcgc catgcctgcc gaaccatgca agccgatgga aggcaatcga
     7021 gtgcacgcga ttcgcgcgcc caacgaaaga cgcgcgcggc cgatgggtga aaccggccac
     7081 gcgcgtccat gttgtgactc tcaggctgct tgcgagcttg ctcgcgacgc gatcgcgatg
     7141 tcgaccactc gacacgcaac cgcaaggtca tgcggccgaa ctagctcttc gccgctgcaa
     7201 tcgacaccaa ctcgatcgcc ttcatgccct tctgctcttt gatatggccc gtgatcgtcg
     7261 actgcttgcc gacccattcc gcgaccgcgg tgctggccga ctcgtggtcc ttgccgatga
     7321 tcaggtagat ggttccgtcc gccgtcagaa agccgatcgg cagccccttc gaaacgcacg
     7381 cctgggcgca cttcgcgtgt tcggcgccgg tagcggactc gggatgcccc atgtagcaat
     7441 agagatccag gacttccccg gtcagggtcg tgggagcggc ctttgccttc ttcgcgtcgt
     7501 cgccggccag cgcaatcgca ctggtcgcca aaacgatcgc catcgccaca aacaacgtct
     7561 tcttcatgtc gactcctcct cagagtaatt gggtgtcgac gcatcgacac cctggtttga
     7621 acaagctagc actttcaaat tacatacaca actaccgaat agcgcgatca cacctccccc
     7681 ttgcgcagcc gcagggcgtt tccgatcacg gataccgagc tgaagctcat tgccgccgcc
     7741 gccacgatgg ggctcaagag aagtccgaag gcggggtaca agaccccagc cgccagcggc
     7801 actcccgcgg cgttgtacac gaacgcgaag aacaggttct gcttgatgtt ggcgatcgtt
     7861 tcccggctca agcgccgcgc gcgcgcgatc ccccggaggt cgcctttgac cagcgtaacg
     7921 cccgcgctct ccatggcgac gtcggtgccg gtgcccatcg cgatgcccac gtcggcctgc
     7981 gccagcgcgg gggcgtcgtt gaccccgtcc cccgccatcg cgaccacgtg cccttcggac
     8041 tgcagacgtt tgacgatgct cgccttctgg tcggggagga cttccgccat gacgtcgccg
     8101 atccccagtt gccgcgccac cgcatgggcg gtcgtggcgc tgtcgccggt gagcatgacg
     8161 acgcgcacgc cgtcgtcgtt caattttcga atcgcgtccg gcgtggtcgg tttgatcgga
     8221 tccgccacac ccaagagccc agcgatgcgt ccgtcgactg cgacgaacat caccgtctga
     8281 ccgctggcgc gcatcgactc ggcgcgctcg gcgaggtcgt cgagccgcgc gccgatcgat
     8341 tccatgagcg cccgattgcc cagcgccacc tcacggccgt tcacgcgtcc gcgcacacct
     8401 tggcccgcga tcgatatgaa cgcctcggcc gtcgcgggcg acacgccgcg ctcgtgcgca
     8461 cccttcacga cggcggccgc gagcgggtgc tcgcttccgc gttcgaggct cgccgcgacg
     8521 tgcaggagtt cgcgttcgtc gaccccgcgc gcttccacgg cgacgagctt cggtcttcct
     8581 tcggtcagcg tgccggtctt gtcgaccacg agggtatcga ctttgcgcaa ggtctcgatg
     8641 gcctccgcat ttcgaaacag cacgccgatg gtcgcgccct tgccggtggc gaccatgatc
     8701 gacatggggg tcgcgaggcc gagtgcgcac gggcacgcaa tgatcaggac cgccaccgca
     8761 ttgacaatgg cgtgggccat gcgcggctcg ggtccggcca gcgcccacac cgcgaaggta
     8821 atcaccgcga ccacgatgac gatgggaacg aagtagcccg agaccatgtc ggcgagcttc
     8881 tggatcggcg cccggctgcg ctgggcttcg gcgaccatcg ccacgatgcg cgcgagcagc
     8941 gtctccgaac cgaccttctc cgcgcgcatg atgaacgaac cgctcgcgtt gacggtcgat
     9001 ccgatcacgc gatcgccggg ttgcttctcg accggcatcg attcgcccgt caccatggac
     9061 tcgtcgaccg cgctcgttcc ctcgagcacg acgccgtcga ccgggacctt ctctcccggg
     9121 cgcacgcgca agcgatcgcc cacgtgcacg tgcgacaacg gaatgtcgtg ctcgtggccg
     9181 tgatcgcaca ggcgacgcgc cgtcgcgggc tggagcccga gcagcttctt gatggccgag
     9241 cccgttcgac tgcgggcgcg cagctcgagc acctggccca gcagcaccag cgtcacgatc
     9301 accgccgccg cttcgaagta gaccgcgacc acgcccccct ctcgaaacga ggccgggaag
     9361 atttgcggca ccagtgccgc gaccacgctg tagacgtacg ccacgccgac gccgagcccg
     9421 atcagcgtga acatgttgag actgcgattg cgcaccgacg ccacggcgcg ggcatagaaa
     9481 ggccacgcgg cccacacgca caccggtgtc gcgagcgcga gttcgatgaa cgtgcgcgcg
     9541 cgcggcgaca cgaggtgcgc tccggtgaag accatgtgac ccatggccaa gaggacgatc
     9601 gggatcgaca gggcgacgga gacccagaag cgtcgcgtca tgtccttgag ctcgggattg
     9661 gtctccggct ccgagccggc ggtgggaatc tccggctcca gcgccatccc gcatttcggg
     9721 caactccccg gcccgctctg gcggacctcg gggtgcatcg ggcacgtgta catcgtcgcc
     9781 atgtgcgtcc ttcctacgcg ctcgacggcg cggccgactt cagttctctg cttcgccaga
     9841 tcaggtagat ggccggatag accaggagct ccatcccgaa gctcgtgaac agacctccca
     9901 ccatgggtgc cgcgacgcgc ttcatcatgt cggcgccggt gccggtgctc cacatgatcg
     9961 gcatcaagcc catgccggcc gccatcacgg tcatcatctt gggccggatg cgcttgaccg
    10021 ccccgtgcac gatggcttcg tggagatcgg cgacggtttt cagtttgccg gcgcgtttgt
    10081 actcgtcgtg gctcatgtcg agaaacagca gcatgaacac gccggtctcg gcgtcgaggc
    10141 cggccagagc gatcatgccg acccacaccg cgatcgacac gttgtagtcg agcaggtaca
    10201 ggaaccacac cgcgcccacc aggctgaagg gtacggccag catgacgatc atggccttca
    10261 tcgtcgactt ggtgttggcg tacaggagca ggaagatcag caggatggtc accggtatga
    10321 cgattttcag ccgttcgcga acgcgcagca tgttctcgta ctggccgctc caaaccagcg
    10381 agtagccggt gggaacttgc agttggccgg cgacggcgcg cttggcttcg tcgacgtagc
    10441 cgccgatatc gcggccggtg atgtccacgt acacgtaggc cgacaacatg ccgttttcgt
    10501 cgcggatcat ggagggaccg ttgaccagct cgatgtccgc cagttcgctc atgggaactt
    10561 gaatgggttc cgccgccccc gcatcgctgc cgcggctccc catgccgtcg ccggcgcgtt
    10621 ccctcatgcc tcccacccgg gccgtgacga gaacccgtcg aagctggtcg aggtcggcgc
    10681 gcaggtcgcg cggatagcgc aagttcaccg agtagcgctc gcggccttcg acggtcgtgg
    10741 tgatgttctc gccgcccacc gcactcatga tgacggcgtg cagatcgtcg atggtgaggc
    10801 cgtagcgggc gagctcccgg cgcttgggga cgaaatccac gaagtagccg ccgcccgcgc
    10861 gttcggcgta gacgctgcgg gttcccggca cctgacgaac gatcccttcc acctgttctc
    10921 cgatgcgctg caattggtcg aggtccgcgc cgaagatctt gatgccgatc ggcgtgcgca
    10981 ccccggtcga gagcatgtcg atgcgcccct tgatgggcat ggtccacgcg ttgacgttgc
    11041 cggggatctg cagcgcgcgg tccatttcgt cgatcagctc gtcccacgag atgtgctccg
    11101 gccatacgtg cccgagcacc tcgcgcaagg gcttcggcca tccgctgaag aaccgctcct
    11161 tgtgccgcca ctgctccttc ggcttgagga caaccgtggt ctccatcatc gagaacggag
    11221 ccggatcggt cgaggtgtcg gcgcgaccgg ccttgcccca aacagtgacc acttcgggga
    11281 agcccttcag gatgcggtcc tcgatctgca tcaatcgctg cgcttcggtc accgacagac
    11341 ccggcagtgt cgtgggcata tagagaatgg cgccctcgtt cagaggcggc atgaactcgt
    11401 gtccgagctt catgtacacc ggaatcgtcg tcgcaaccac caaggccgag agcgcgatca
    11461 ctttgcgagg atgccgcagc accagccggc acggcttctc gtagagccgg aacagaatcc
    11521 tgctgatcgg gtgcttttct tccgggtagt accggcccac cagcgccgtg gtcagcgctc
    11581 gccccagcca gcgcgggcgc gcccggatcg ggtccatgcg cgtgaacatc attcgcaccg
    11641 cgggatcgag cgtcaccgcg agcagggccg ccagcgcgat ggcgagcgtc ttggtgtagg
    11701 ccagcggttt gaacaggcga ccttcctggt cgatcagcgt gaacaccggc agaaacgaga
    11761 ccgcgatcac tagcaacgag aagaacaccg acggcgcgat ctcctggatc gccttgagca
    11821 gggtgtcgtg gtaatcgccc acacgcccct gctcgtgcca gatctccagc ttcttgtagg
    11881 cgttctcgac caagacgatc gcgccgtcca ccagcacgcc gaccgagatc gcgatgcccg
    11941 ccagcgacat gatgttggag gtgacgccca tgtggtacat ggggatgaac gagagcgcca
    12001 ccgtgacggg aatcgtgatg atgggcacga aggccgacgg tccgtggaac agaaagaaca
    12061 tgatcacgag gctgacaatg atcatctcga cgatcagctc gtgcttgagg ttgtcgatcg
    12121 attccttgat cagatgggag cggtcgtagg tggagacgac ttccacgccc tccggaagcg
    12181 acggcttcag ctcttccagc ttgctcttca cgcgctcgat cacattgagc gcgttttccc
    12241 cgtggcgcat cacgatgatg ccgccgacgg cgtcgccgtc gccgtcgaag tcggcgacac
    12301 cgcgccgcag ctgcgggccg agcgcgatgg tggccacgtc gcgcaagagc accgctgtcc
    12361 cctgcgtact ggtcttgacc accacgtctt cgagcgccgc ggtgtcttgg atgtagccgc
    12421 gcgcccgcac catgtactcg cgcccgctcc actcgatcaa gcggccgccg acttcgttgt
    12481 tcgacttgcg gaccgcttcg atgatctcca tcatgccgag gccgtagctt tgcaggcggt
    12541 tgggatcgat ggtgatctgg tactgcttct ggaatccgcc gatgccggcc acttccgcga
    12601 cccccgcgac cgattgcagc gcgaagcgca gattccagtc ctggaagctg cgcaggtcgg
    12661 cgaggctgtg cttccccgtc ttgtcgacca gtgcgtactg atagacccaa cccactccgg
    12721 tcgcgtcggg accgagcgaa gggttgacgc cgggcggcag ggtaccggtg atcttctgca
    12781 ggtattcgac cacgcggctg cgcgcccagt agatgtcggt gccgtcctgg aagatgacgt
    12841 atacgtagct gaagccgaaa tcgctgaagc cgcggatcgt cttcaccttc ggcgcaccga
    12901 gcagcgccgt gatgatcgga tacgtgacct ggtcttccat gatgtccggg ctgcgatccc
    12961 acttcgtgta gacgatgacc tgggtgtccg agaggtccgg gatggcgtcc aagcggatgt
    13021 gccgcatggt gtagaacgcg tacacgagcg cggcggccgt gagcatgatc acgagcacgc
    13081 gattggccgc ggagaatgcg atccacttgt ggatcagtcc gttgtgttga ggagaggacg
    13141 tattcatcgg tctctagcgg cgatgtccct cgtgctcatc ccccgcgcgc gcggagtcgg
    13201 cgccggcgga ca
//
LOCUS       DASPGK010000263         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_535718, whole genome shotgun sequence.
ACCESSION   DASPGK010000263 DASPGK010000000
VERSION     DASPGK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535718"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VEC56_07175"
     CDS             <1..488
                     /locus_tag="VEC56_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXV13972.1"
                     /translation="SIIADARNPENKSVVNLKIKFRESFRPFAPSVLEEKCSEWFDLD
                     VPSPYMLLVADVREGKRTIPSVTHVDHSARIQTVDEHSLPRYYKLIKRFDEKTGCPVI
                     INTSFNVRGEPIVCTPHEAYLCFMRTNMDVLVLDRFVMYKPDQKPLKEDVDWRTLFAL
                     D"
     gene            498..>2395
                     /locus_tag="VEC56_07180"
     CDS             498..>2395
                     /locus_tag="VEC56_07180"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV13973.1"
                     /translation="MKGRKATPRRHPALTPKDLRWECNASRLGFETTDELEFKPNIIG
                     QDRALDAIRLGLSIRSPGYNIFVSGLTGTGKLTAIRYMLEGMDLRRDDLVDICYVHNF
                     TAEDTPVCIVLPAGFACKLRARMKDLLDDVVNLIPGALQSERFKRQQARIADEVKKTR
                     DDMLQKLEKEVNENGFSMVQVEYEGYTRPEIVPLVAGEPTPIDRLTSLLVQGKVKQGD
                     YEKFQAAHSTLSRKLDDFLMATRDLHRTLDQRTADLEKQYVAPLVDGELAAIAKEFDS
                     GKVSRFLQDVRTYILDHLPMFVVPKAEIAKKRREFLPFDVNVLVDNAGRREAPVVIET
                     SPTYVNVFGTIERYVGPDGDHWTDHTAIRGGSLLRANGGYLIMNMVDVFEEPMVWVAL
                     KRALKSQRHVIRGFDSLLLMPIASIKPEAVELDMKIVLIGEAWMYQALWDYDEDFRTV
                     FKVKADFDTVMPNTIANQKRYCRFIRVLTRVEGLPAFHSGACGLVIEEGTRLAGRRDR
                     LSTRFSEMGDLIREAAHWATKDGAKVVRAAHVAKAVAERVRRVSLIEDKVQEMYDDGS
                     ILIDTSGAKVGQVNGLAIYDVGDHVFARPSRITAETGVGRAGVINIEREADMSGKIHN
                     KGVLILEG"
BASE COUNT          463 a          778 c          760 g          394 t
ORIGIN      
        1 gctccatcat cgcggatgcg cgcaaccccg agaacaagag tgtggtcaat ctcaagatca
       61 agttccgcga gagcttccgg cccttcgcgc cctcggtgct ggaggagaag tgcagcgagt
      121 ggttcgacct cgacgtaccc agcccgtaca tgctgctggt cgcggacgtt cgcgagggca
      181 aacgtaccat cccgtcggtg acgcacgtcg accactcggc ccgcatccag accgtcgacg
      241 aacactcgct cccgcgctac tacaagctca tcaagcgctt cgacgagaag accggctgcc
      301 cggtgattat caacacgtcg ttcaacgtgc gcggcgagcc catcgtgtgc acgccccacg
      361 aggcctacct ctgcttcatg cgcaccaaca tggacgtact ggtgctggac cgtttcgtca
      421 tgtacaagcc cgaccagaag cccctcaagg aagatgtgga ctggcgcacg ctgttcgcgc
      481 tggattagac tgcgtccatg aagggccgca aggcaacccc gcgccggcac ccggcgctca
      541 caccgaagga cctgcgctgg gagtgcaacg cctcccgcct gggcttcgag accaccgacg
      601 agctcgagtt caagcccaac atcatcgggc aggaccgcgc gctggacgcg atccggctgg
      661 gcctctcgat ccgtagcccc ggttacaaca tcttcgtctc cggcctgacc ggaaccggca
      721 agctcaccgc catccgctac atgctcgaag gcatggacct tcggcgcgac gacttggtcg
      781 acatctgcta cgtgcacaac ttcacggccg aggatacgcc cgtgtgtatc gtgctgccgg
      841 cgggtttcgc gtgcaagctg cgcgcgcgta tgaaagacct gctcgacgac gtcgtcaact
      901 tgattcccgg cgcgctccag agcgagcgtt tcaagcgcca acaggcccgc attgcggacg
      961 aagtcaagaa gacgcgcgac gacatgctgc agaagctcga gaaggaagtg aacgagaacg
     1021 gcttctcgat ggtgcaggtc gagtacgaag gctacacgcg tcccgagatc gttccgctgg
     1081 tcgccgggga accgacgccg atcgaccggc tgaccagtct cctggtgcag ggaaaggtca
     1141 agcagggcga ctacgagaag ttccaggccg cccactccac cctttcgcgc aaactcgacg
     1201 acttcctgat ggccacgcgc gatctccatc gcacgctcga ccagcgcacc gccgatttgg
     1261 agaagcaata cgtggccccg ctcgtggacg gggaattggc cgccatcgcc aaggaattcg
     1321 actcgggcaa agtgtcgcgc tttctccagg acgtacggac gtacatcctc gaccacttgc
     1381 cgatgttcgt ggtccccaag gcggagattg ccaagaagcg ccgcgagttc ctgcctttcg
     1441 acgtcaacgt cctggtcgac aacgccgggc ggcgcgaagc gcccgtggtc atcgagacct
     1501 cccccaccta cgtcaacgtc ttcggcacca tcgagcgtta cgtcgggccg gacggcgacc
     1561 actggaccga ccacaccgcc atccgcggcg gctcgctgtt gcgcgcgaac ggcggctact
     1621 tgatcatgaa catggtcgac gtgttcgagg agccgatggt gtgggtggcg ctgaagcgcg
     1681 cgctcaagag ccagcgccac gtcattcgcg gcttcgattc gctcctgctg atgccgatcg
     1741 cgtccatcaa gccggaggcg gtcgagctcg acatgaagat cgtgctgatc ggcgaggcgt
     1801 ggatgtacca ggcgctgtgg gattacgacg aggacttccg caccgtgttc aaggtcaagg
     1861 cggacttcga caccgtgatg cccaatacga tcgccaacca gaagcgctac tgccgcttca
     1921 ttcgcgtcct cacccgcgtc gagggcctgc ccgcgttcca cagcggcgcc tgcggcttgg
     1981 tgatcgaaga gggcacgcgc ctggcgggtc gccgtgaccg cctttcgacg cgcttcagcg
     2041 agatggggga cctgattcgc gaggctgcgc actgggcgac caaggacggg gccaaggtgg
     2101 tgcgcgcggc gcacgtggcc aaggcggtcg cggagcgggt gcggcgcgtg agcctgatcg
     2161 aggacaaggt gcaggagatg tacgacgacg gttcgatcct catcgacacc tccggagcca
     2221 aggtgggcca ggtgaacggg ctcgcgatct acgacgtcgg cgaccacgtg ttcgcgcggc
     2281 cgagccgcat caccgcggag acgggggtgg ggcgcgcggg tgtcattaac attgagcgcg
     2341 aggccgacat gagcggcaag atccacaaca aaggcgtgct gatcctggag ggcta
//
LOCUS       DASPGK010000264         4914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1589841, whole genome shotgun sequence.
ACCESSION   DASPGK010000264 DASPGK010000000
VERSION     DASPGK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589841"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..429
                     /gene="rpsK"
                     /locus_tag="VEC56_07185"
     CDS             19..429
                     /gene="rpsK"
                     /locus_tag="VEC56_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXV13974.1"
                     /translation="MTTNKPAAGAKRPASKKKLRKVDAYGVAHIKSTFNNTIVTITDS
                     TGAVVSWASPGMVGFKGSRKSTPFAAQLAAERCAREALELGMRKVEAWIRGPGAGREA
                     AIRSLRAAGLEVTAVKDCTPQPHNGCRPRKRRRV"
     gene            457..1086
                     /gene="rpsD"
                     /locus_tag="VEC56_07190"
     CDS             457..1086
                     /gene="rpsD"
                     /locus_tag="VEC56_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXV13975.1"
                     /translation="MARYRDAKCKLCRREGEKLFLKGDRCYSNKCAIEKRNSPPGQHG
                     KARVRKSSKYNVQLREKQKLRKTYGLLERQFRNYFHKAAAQKGITGTTLLQLLERRLD
                     NVVYRLGFTTSRAQARQLIRHNHFEVNGKKVNIPSYLVSPGDRIGPHGKSRDLFIIRE
                     AIKNVDARQAVPYLDLDTNKLEGVFTDIPSRDVIPVPVDEQLIVELYSK"
     gene            1138..2190
                     /locus_tag="VEC56_07195"
     CDS             1138..2190
                     /locus_tag="VEC56_07195"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXV13976.1"
                     /translation="MKWRNLLMPKEIVRDDATATPTNARFIVEPLERGFGLTLGNALR
                     RTLLSSIQGAAVSAVRIKGVLHELSNIPGVVDDVTDVVLNLKQLVVVMHNDDPKFLKL
                     KVTKQGPIRAGDITEDPDIEIVNKDLVICTASENVEVEMDILVGHGRGYVPGETHDLG
                     DYDIGLIPMDSNFSPIRKVAYTVENTRVGQRTDYDRLILEITTNGSITPEDALGYAAK
                     ILKDHMVLFIKFDEAPMEAQEPVVDEERDRLRELLGRSVEELELSVRSGNCLRRANIK
                     TLGDLVRRSEAEMLKYRNFGKQSLKEITEILSGMGLGLGMDIDALLGGRAPKPAAPAA
                     PMTDDDTEIEAEAENA"
     gene            2210..2857
                     /gene="rplQ"
                     /locus_tag="VEC56_07200"
     CDS             2210..2857
                     /gene="rplQ"
                     /locus_tag="VEC56_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXV13977.1"
                     /translation="MRHGKDHRKLGRTAAHRRAMMRSMVTSLFASERITTTAPKAKEA
                     RRVAEKLITRAKKGHRAHKEGAAAVALAHWREAGRYVRSTVVLRKLFDQLAPLYMERN
                     GGYTRIIRMDHTRVGDNARMVMLELVDSEISKKPLIRGAAAETQGESDAKAAKAAGRG
                     KAKEKAEAAAETKPKKEKKTKPGRVAKESKPAKTKADTKKKDSGASKRSAPKKSG"
     gene            2905..4197
                     /locus_tag="VEC56_07205"
     CDS             2905..4197
                     /locus_tag="VEC56_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HXV13978.1"
                     /translation="MIAAIWLGSAVRAPRYEKPLSSRGAFSFAPAPAHADTALFPSPY
                     LASLRESTPILPDDEIRALWVVRDALTSPESVARVVDFAVLTRTHMLFVQVRGRADSF
                     YRSAIEPASPILEAPVADFDPLAYLLTLAHRNGIVVHAWLNVFLVWSDMSKSPPSGHL
                     GARNPDWLLTDAKGVRMDRMPSSRWKRAGVEGWFVSPGNRAMRAHMARVVHELVTEYE
                     IDGIHLDYIRYPNRDLSFDPTTRAEFAVKWGVDPAEAAHGDRGGLQRVIGAGALAVVD
                     SLYNESRVAQVDSMVIAIRAACRDKALSAAVVADPFTARHDKGQDWARWVNNGWMDFV
                     VPMAYNFPPLELEHRATVYNRMVGPERWLMGLGVFDGREEYLAESVELLREVGVMGFS
                     IFSYNVLEKEGFGAALIEEAVLPPDTSYVDEEEDESDE"
     gene            complement(4274..>4914)
                     /locus_tag="VEC56_07210"
     CDS             complement(4274..>4914)
                     /locus_tag="VEC56_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13979.1"
                     /translation="IDNGEAALKLSRDAGSANLALYGYWGAWKNPYGFSPADSVVGTP
                     SHYYYPALNAYGASVRGSLFGGVAWVEGGWYDSADDRDGDDPLVPNSEARAMAGYERQ
                     WWSDFTGGAQVYWEGVQDYRASEYVKDENYTLLTVRLTQLLRYQTVKLSVFTFYSPSD
                     EDAYARLAVGYDYTDELNLTVGANLFQGDDPRTLFAMNEDNSNAYVRVRYSF"
BASE COUNT          972 a         1566 c         1586 g          790 t
ORIGIN      
        1 cgctaaggag gaatccgttt gacgaccaac aaaccggctg cgggcgccaa gcgcccggcc
       61 agcaagaaga agctcaggaa ggtcgacgcg tacggtgtcg cgcacatcaa gtcgaccttc
      121 aacaacacca tcgtgaccat caccgattcg acgggggccg tggtgtcgtg ggcgagcccg
      181 ggcatggtgg gtttcaaagg atcgcgcaag agcacgccgt tcgccgccca gttggcggcc
      241 gagcgctgcg cgcgcgaagc gttggaattg ggaatgcgaa aggtggaagc gtggatcagg
      301 ggaccgggcg cgggccgcga ggccgcgatc cggtcgctgc gcgcggccgg gctggaagtg
      361 accgcggtca aggactgcac gccccagccg cacaacggat gccgccccag gaagcggcgg
      421 cgcgtgtagc ctcgcggcta cccgcttgga ggatgaatgg ccaggtatcg tgacgcaaag
      481 tgcaagctgt gccgtcgaga gggcgaaaag ctcttcttga agggcgaccg ttgttactcc
      541 aacaagtgcg cgattgagaa gcgcaactcc ccgccgggtc agcacggcaa ggcgcgggtg
      601 agaaaatcgt cgaagtacaa cgtgcagttg cgcgagaagc agaagctgcg caagacctac
      661 ggcttgctcg agcggcagtt ccgcaattat ttccataaag cggctgccca gaagggcatc
      721 accggcacga ccctcttgca gctgctggag cggcggctgg acaacgtcgt ataccggttg
      781 ggattcacga cctcgcgtgc ccaggcgcgc cagctcattc gtcacaacca tttcgaggtc
      841 aacgggaaga aggtcaatat cccgagctac ctggtatcgc cgggcgaccg catcggcccg
      901 cacgggaaga gccgcgatct gttcatcatt cgcgaggcga tcaagaacgt ggacgcccgc
      961 caggcggtcc cgtatctcga cctcgacacg aacaagctcg aaggcgtctt caccgatatc
     1021 ccgagccgcg acgtcattcc ggtgccggtg gacgagcaac tgatcgtcga gctctactcg
     1081 aagtaatcac ccgcgggccc cttcaacggg gttatcgcag gacggaggaa cggaagcatg
     1141 aaatggcgta atctattgat gccgaaggag atcgttcgcg acgacgcgac cgccacgccc
     1201 accaacgcgc gcttcatcgt ggagccgctg gagcggggct tcggcttgac cctcggcaac
     1261 gcgctgcggc gcacgctgct ctcgtccatt cagggcgcgg cggtgtcggc cgtccgcatc
     1321 aagggcgtgc tgcacgagct ctcgaacatc cccggtgtcg tcgacgacgt caccgacgtg
     1381 gtgctgaact tgaagcagct cgtcgtcgtg atgcacaacg atgatcccaa gtttttgaag
     1441 ctcaaggtca ccaagcaggg accgattcgc gccggcgaca tcacggaaga cccggacatc
     1501 gagatcgtca acaaggacct cgtgatctgc acggcgtccg agaatgtcga agtcgagatg
     1561 gacatcctgg taggacatgg gcgcgggtac gtacccggcg agacccacga cctaggcgac
     1621 tacgacatcg gcttgatccc gatggactcg aacttctcgc ccatccgcaa ggtggcatac
     1681 acggtcgaga atacgcgtgt gggccagcgt accgactacg atcgtttgat cctcgagatc
     1741 acgaccaacg ggagcatcac gcccgaggac gcgcttggct acgcggctaa gatcctcaag
     1801 gatcacatgg ttctcttcat caagttcgac gaggcgccca tggaggcgca ggagccggtg
     1861 gtcgacgaag agcgcgaccg cctgcgcgaa ctcttgggcc ggagcgtcga agagctggag
     1921 ctttcggtgc gctcgggcaa ttgcctgcgc cgcgccaaca tcaaaacgct cggcgatctg
     1981 gttcgccgca gcgaagccga gatgctcaag taccgcaact tcggcaagca gagcttgaag
     2041 gagatcacgg agatcctctc gggcatgggc ctggggctcg gcatggacat cgacgcgctc
     2101 ctcggcgggc gcgcacccaa gccggcggcc cccgcggctc cgatgaccga tgatgacacc
     2161 gagatcgagg ccgaagcgga aaacgcgtag tcaatagaag gacagttcca tgagacacgg
     2221 caaagatcat cgcaagctcg gacgcaccgc cgcccaccgt cgcgccatga tgcgcagcat
     2281 ggtgacgtcg ttgttcgcct cggagcgcat caccaccacc gcacccaagg cgaaggaagc
     2341 ccggcgcgta gcggagaagc tcatcacgcg cgccaagaag ggacaccgcg cccacaagga
     2401 aggtgccgcg gccgtggcgc tcgcgcattg gcgcgaagcc ggtcgctacg tgcgcagcac
     2461 cgtcgtgctc cggaaactct tcgatcagct ggcgccgctc tacatggagc gcaacggcgg
     2521 ttacacgcgc atcattcgca tggaccacac ccgtgtcggc gacaacgcgc gcatggtgat
     2581 gctcgagttg gtcgactccg agatcagcaa gaagccactc atccgcggcg cagccgcgga
     2641 gacccagggc gaatcggacg cgaaggcggc gaaggctgct ggacgcggaa aggccaagga
     2701 gaaggccgaa gccgccgccg agaccaagcc caagaaggaa aagaagacca agccgggccg
     2761 ggtcgcgaag gaaagcaagc ccgcgaagac caaggccgac accaagaaga aagactcggg
     2821 tgcgagcaag cggtccgctc ccaagaagtc cgggtagttt accgaatgtc gcgcggacgc
     2881 cgagccgcag caaccgtttc ggtcatgatc gccgcgatct ggctgggttc cgctgtacgc
     2941 gcgccgcggt acgaaaagcc cctctcgtcg agaggggctt tttcgtttgc ccccgcgccc
     3001 gcgcacgccg acaccgcgct ctttccctca ccctatctcg cgagcctccg cgagagcacg
     3061 cccatcctcc ccgacgacga gattcgggcg ctttgggtcg tgcgcgacgc cctcaccagc
     3121 cccgagtcgg tcgcgcgcgt ggtcgacttc gcggtgctga cgcgcacgca catgctcttc
     3181 gtccaggtgc gcggacgcgc cgattcgttt tatcgttccg cgatagagcc ggcctcgccc
     3241 atcctcgaag cgcccgttgc cgacttcgac ccgctggcgt acttgctcac cctcgcgcat
     3301 cgcaacggca tcgtcgtgca cgcgtggctg aacgtcttcc tggtgtggtc cgacatgtcg
     3361 aagtcgccgc cgtcaggcca cctgggcgca cggaatcccg actggttgct gaccgacgcc
     3421 aaaggcgtgc gcatggaccg catgccgtcg tcgcgctgga agcgcgccgg cgtggaaggc
     3481 tggttcgtct cgccggggaa ccgtgccatg cgcgcgcaca tggcgcgcgt cgtccacgag
     3541 ctcgtgaccg agtacgagat cgacgggatt catctcgact acattcgcta tcccaaccgc
     3601 gatctctcgt tcgaccccac tacccgcgcg gagttcgccg tcaagtgggg cgtcgatccc
     3661 gccgaagccg cgcacggcga ccgcggcggg ctccagcgtg tgatcggtgc gggagcgcta
     3721 gcggtcgtcg acagcctcta taacgaatcg cgtgtcgcgc aagtcgacag catggtgatc
     3781 gcgatccgcg ccgcgtgccg agacaaggca ctctcggccg ccgtagtcgc ggaccccttc
     3841 accgcgcgcc acgacaaggg gcaggactgg gcacgctggg tcaacaacgg ctggatggac
     3901 ttcgtggtgc cgatggccta caacttcccg cccctcgagc tcgagcaccg cgcgaccgtc
     3961 tacaatcgca tggtcgggcc agagcgctgg ctcatgggcc tgggcgtctt cgacgggcgt
     4021 gaggagtacc tcgccgaatc cgtggagtta ttgcgcgaag tcggcgtgat gggcttctcg
     4081 atcttctcgt acaacgtgct ggagaaggaa ggcttcggcg cggcgctgat cgaggaagcc
     4141 gtgctcccac cggacacgag ttacgtggat gaggaagagg acgagtccga cgagtgaact
     4201 cgtgcggaac tgatgtgcaa gcacaatgat gggttgtgag tagtcgaggc gcgggcgcgg
     4261 agtctctccc cgcctagaag ctatagcgca cgcggacgta ggcgttgctg ttgtcctcgt
     4321 tcatcgcgaa caacgttcgc ggatcgtctc cctggaacag gttcgcgcct acggtgaggt
     4381 tcaactcgtc cgtgtagtcg tatcccaccg cgagccgggc gtaggcgtct tcgtcgctgg
     4441 gagaatagaa cgtgaacacc gacagcttca cggtctggta gcgaagtagc tgcgtgaggc
     4501 gcacggttag cagcgtgtag ttctcgtcct tgacgtactc gctcgcgcga tagtcctgca
     4561 caccctccca atagacctgc gcgccgccgg tgaagtcgct ccaccactgg cgctcgtagc
     4621 cggccattgc gcgtgcctcg gagttgggaa ccaagggatc gtcgccgtcg cggtcgtcgg
     4681 cggagtcata ccatccgcct tccacccacg ccacaccgcc gaagagacta ccgcgcacgc
     4741 tcgcgccata tgcgttcaac gcggggtagt aatagtgcga cggggtgccc acgacggaat
     4801 ccgcgggcga aaacccgtag ggattcttcc atgcacccca gtaaccatag agagcgagat
     4861 tcgcgctgcc ggcgtcgcga ctcaacttga gcgcggcctc gccgttgtcg atcg
//
LOCUS       DASPGK010000265         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_184551, whole genome shotgun sequence.
ACCESSION   DASPGK010000265 DASPGK010000000
VERSION     DASPGK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184551"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VEC56_07215"
     CDS             <1..1028
                     /locus_tag="VEC56_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV13980.1"
                     /translation="PLLGRVLITGGCGFIGRAIASRLAHSQCDVVLLDDLSTGVVNGL
                     ARRRHVKIRVGSVLDPSAVREAARGCDLVLHLASLVGKRLVSERRELAYATSVGGAQN
                     ILDHTGDIPVVLFSSSAVYGVEAAGTVGEAAAIDDQGALDYDGGFRGYALGKLHMERL
                     AANHARNGRRVLIVRPFNIVGPGQTGRYGMVIPRFVKSALRDRPLMVYGDGRQTRSFS
                     HVRTFVSVILRLIATRDAWRPPQVPVNIGTPRATSIQRLAEVVLRVTSSRSPVRYVAY
                     DTVYTGQRDVLERNPDVTRCQTLVGSVRWPGIDAIVRDVARDESPHPRAHRDEAMPAL
                     RPSDGEA"
     gene            1025..2377
                     /locus_tag="VEC56_07220"
     CDS             1025..2377
                     /locus_tag="VEC56_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV13981.1"
                     /translation="MTRTDLERDFIVLGAGVAGLAAASELGESCTVFEREDRPGGLVR
                     TDCIDGYWFDRVLHLLHFQDDAMERRMRDLLGDALVRLSPEAWVETRHGTVRFPFQLH
                     LAGLAKDVRERCLSDFVVAASEHNDEQPRNFEEMLLRTFGREMCDVFLFPYNRKLWKR
                     PLAALAPSGFQWNITRPDVESVARGAASIDARAGAYNSNGWYPRPRADAPQRGMAVLS
                     EALAARVADLQLEHEVQVIDPVTRSVVVRHRGRDVRARYRQACLATLPLSRLLSMVSG
                     APADLVDACRRLHRNRVISVMVRVAGPRPSGQGLWRYYADESIVFTRLIFMHEFDPLS
                     APEHGWGLMAEITEPAEQPLADSRVVIRRVIEDIARARVLSPECGIVGATARVVDPAY
                     VVFDLASADVVAEALAFLRSCDVIAIGRYGKWEYSSMAQVMRDGFAWAESVAAREKPA
                     "
     gene            2374..>3120
                     /locus_tag="VEC56_07225"
     CDS             2374..>3120
                     /locus_tag="VEC56_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13982.1"
                     /translation="MTEVLAPLAFAALAFVLGAGATRVVRAIAMATGAVNRPNPIVPQ
                     HTRPIAYLGGVAIAVALAIAAVIASLVARDDPLWLATARGAIPAGLFLVGGTFDDARP
                     LAPLPKFAFQSAAAATAVLFGLVWRLTGVAWLDAAITMLWLVAIVNAFNLVDVCDGLL
                     AGLAAIALLFLFVVAGHDASVLLVLGVVLGFLVFNFPDASIFLGDGGSHLLGFLVAAL
                     AILVQPATWTNAVESLLIVGVPVFELVFLIV"
BASE COUNT          447 a         1015 c         1057 g          601 t
ORIGIN      
        1 cccctctctt ggggcgggtg ctgatcaccg gcgggtgcgg cttcatcggg cgcgccattg
       61 cgtcgcgtct cgcccattcg caatgcgacg tcgtccttct cgacgatctc tcgaccgggg
      121 tcgtgaatgg tctcgcacgc cggcgccacg tgaagatccg tgtcggttcg gtgctcgacc
      181 catcggcggt gcgtgaggct gcgcgcggct gtgatctcgt cctgcacctg gcgagtttgg
      241 tgggaaagcg gctcgtctcc gaacgccgcg aactcgccta cgcgaccagc gtgggcggtg
      301 cccagaatat cctcgatcac accggagaca ttcccgtcgt cttgttttcg tcgtccgcgg
      361 tgtacggcgt cgaagccgcg ggaaccgtgg gcgaagcggc ggcgatcgac gaccaaggcg
      421 cactcgacta cgacggcggc tttcgcggat acgcgctggg caagctccac atggagcgcc
      481 tcgcagcgaa tcatgcgcga aatggacgac gggtgctaat cgtgcggcca ttcaacatcg
      541 tcgggccggg acaaaccggt cgctacggaa tggtgatccc tcgcttcgtt aagagcgcgt
      601 tgcgcgaccg gccactcatg gtttacggcg acggcaggca gacacggtcg ttctcgcacg
      661 tgcgaacctt cgtcagcgtc atcctgagac tgattgccac tcgcgacgcg tggcgcccac
      721 cgcaggtgcc ggtcaacatc ggaaccccgc gggcgacgtc gatccagcgc ctggcggagg
      781 tcgtgctgcg cgtcacgtcg tcgcgttcgc cggttcgata cgtggcctac gacaccgtgt
      841 acaccggcca gcgcgacgtt ctcgagcgca acccggacgt aacgcgatgc caaacgctgg
      901 tgggatcagt gcgatggccc ggcatcgacg ccatcgtgcg cgacgtggcg cgcgacgaat
      961 caccccaccc gcgcgcgcac cgcgatgaag cgatgcctgc actccgtccg tcggacggcg
     1021 aggcatgacg cgtacggatc tcgaacggga cttcatcgtc ctgggcgcgg gcgtcgcagg
     1081 tctggcggcg gcgtcggagc tcggcgagag ctgcaccgtg ttcgagcgcg aagatcgccc
     1141 gggcggtttg gtgcgcacgg attgcatcga cgggtattgg ttcgatcgcg tgctccactt
     1201 gctccacttt caggacgacg ccatggagcg acgcatgcgt gatctgctcg gcgatgcgtt
     1261 ggtgcgcctg agtccagagg cgtgggtcga gacgcgacac ggaacggtac gattcccgtt
     1321 ccagctccac ctcgcgggtc tcgcgaagga cgtgagggag cgatgcctgt ccgatttcgt
     1381 cgtcgccgcc tcggaacaca acgacgaaca accgcgcaat ttcgaagaga tgctcttgcg
     1441 gaccttcggg cgcgagatgt gcgacgtctt cttgtttccc tacaatcgaa agctctggaa
     1501 acgccccctg gccgcgctcg ccccttcggg ctttcagtgg aacatcacgc ggcccgacgt
     1561 cgagagtgtc gcacgcggtg ccgcctccat cgatgcgcgc gcaggcgcct acaattccaa
     1621 cggttggtac ccgcgtcctc gcgccgatgc accgcagcgg gggatggcgg tcttgtcgga
     1681 ggcactggcg gcgcgtgtcg cggacctgca actggagcac gaggtacagg tcatcgatcc
     1741 ggtaacgcgg tcggtcgtgg tcagacaccg cgggcgcgac gtgcgcgcgc gctatcgaca
     1801 ggcctgcctc gctacgctgc cgctgtcgcg cttgctctcc atggtctccg gtgcgcccgc
     1861 cgacttggtc gacgcatgcc ggcgcttgca ccgcaatcgc gtcatttcgg tcatggtgcg
     1921 cgtcgcgggg ccgcgtccga gcgggcaagg tctctggcgc tactacgcgg acgagtcgat
     1981 cgtcttcacg cgtctcatct tcatgcacga gttcgacccg ctctccgcgc cggaacacgg
     2041 ctggggactg atggcggaga tcaccgaacc cgcggagcag ccgctggctg atagccgcgt
     2101 cgtgatcagg cgcgtcatcg aagacatcgc gcgcgcgcgc gtcctatccc cggagtgcgg
     2161 gatcgtcggg gccaccgcgc gcgtcgtcga tccagcctat gtggtcttcg acctcgcctc
     2221 ggccgacgtc gtggcggaag cactcgcgtt cctgcggtca tgcgacgtga tcgcgatcgg
     2281 tcgttacgga aagtgggagt actcgtcgat ggcgcaagtg atgcgcgacg ggttcgcgtg
     2341 ggcggagtcc gtggccgccc gggagaagcc ggcgtgacgg aagtgctcgc gccgctcgcg
     2401 ttcgccgcgc tggccttcgt attgggcgcg ggtgccacga gggtcgtgcg cgcgatcgcg
     2461 atggcgacgg gtgccgtcaa tcgccccaac cctatcgttc cgcagcacac tcgtcccatc
     2521 gcgtatctgg gcggagtcgc catcgcggtc gcactcgcga tcgcggcggt gatcgcaagc
     2581 ctcgtggcgc gcgacgaccc actgtggttg gccacggcgc gcggcgccat tcccgcgggc
     2641 ttgttcctcg tggggggaac attcgacgac gcgcgcccgc tggcaccgtt gccgaagttc
     2701 gcatttcagt cggccgcggc cgcgacggcg gtactctttg gtttggtgtg gcgactcacc
     2761 ggagttgcgt ggctcgacgc cgctatcacc atgctgtggc tggttgccat cgtgaacgcg
     2821 ttcaacctgg tggatgtttg cgacggcctg ctggctggcc tggccgccat cgctttgctc
     2881 ttcctgttcg tggtcgcggg gcacgacgcc tccgtgctgc tagtgttggg cgtcgtcctg
     2941 gggtttctcg ttttcaattt tcccgacgcg tcgatcttcc tcggcgacgg cggcagtcac
     3001 ctgctcggat ttctcgtggc cgcactcgcg atcttggtgc agccggccac gtggacgaac
     3061 gccgttgaat cgctcttgat cgtgggcgta ccggttttcg agctggtctt tctgatcgtg
//
LOCUS       DASPGK010000266         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_770348, whole genome shotgun sequence.
ACCESSION   DASPGK010000266 DASPGK010000000
VERSION     DASPGK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770348"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VEC56_07230"
     CDS             <1..834
                     /locus_tag="VEC56_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXV13983.1"
                     /translation="GSAVADFFQPPKTVVGAASPQTAATIVALYEGIQAPVFATSIRV
                     AETVKYVDNVFHATKITFANEIGTVCKAAGVDSHDVMRIFAADTKLNISAAYLKPGFA
                     FGGSCLPKDVRALVYYARAHDLNLPLLSSLLPSNDVQIDRAAEMIFAQGKKRLGFLGL
                     SFKPGTDDLRESPLVRLIEICLGKGYEIRIYDQSVSLARLVGANKAYIEKEIPHISRL
                     LANDLAEVAAFADVLVVGHRSPDFADAARRASSGKKVVVDLARVFDAPPDDRSYLGFN
                     W"
     gene            complement(848..1324)
                     /locus_tag="VEC56_07235"
     CDS             complement(848..1324)
                     /locus_tag="VEC56_07235"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXV13984.1"
                     /translation="MIARILTGRELNLVLRLFLGGMFLYAAWDKVLQPAGFAMSVRGY
                     KIIPFALSNLFALGVSWTELIAGLMLILGIFTRKAAGAIAILLVMFIVAISMVIVRGM
                     TVDCGCFGSEGGSSTSWLLIVRNIALLAGAYLIMRYNDGFASVFPGGRSSDARLSE"
     gene            complement(1321..1851)
                     /locus_tag="VEC56_07240"
     CDS             complement(1321..1851)
                     /locus_tag="VEC56_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV13985.1"
                     /translation="MKNALSQMVGGLGVMVVATLMGVVVNAVRPNGVALIQSGAPVST
                     AQHPAVHADSAAADTTHALAEGAISLAEMKRLFDEGSAIILDARDAAEYEQGHIPGAI
                     NIPYDRIPEYFDILNSQVPMDAHVVVYCRSLTCDFSDQLATELKIIGYQNVSVFSGGW
                     DQWTTAGYPIEGMVDK"
BASE COUNT          395 a          663 c          678 g          361 t
ORIGIN      
        1 ggcagtgccg tggccgattt tttccaaccg ccaaagaccg tcgtcggcgc cgccagcccg
       61 cagaccgccg ccaccatcgt cgcgttgtac gaaggcatcc aggcccccgt gttcgcgacc
      121 tcgatccgcg tggccgagac cgtcaagtat gtcgacaacg tgtttcacgc caccaagatc
      181 accttcgcga acgagatcgg gaccgtgtgc aaggccgcgg gtgtcgacag ccacgacgtg
      241 atgcgcatct tcgcggccga caccaagctc aacatctcgg ccgcctattt gaaaccgggg
      301 tttgcgttcg gggggtcgtg tttgcccaag gatgtgcgcg cgctcgtcta ctacgcgcgc
      361 gcccacgacc tcaatcttcc gctgctctcg tcgctgctcc cgagcaacga cgtgcagatc
      421 gatcgcgccg ccgaaatgat attcgcgcag ggcaagaagc ggctcgggtt cctgggtctg
      481 agtttcaagc ccggcaccga cgacttgcgc gagagcccgc tggttcgttt gatcgaaatc
      541 tgcctcggca aggggtacga gatccgcatc tacgaccaga gcgtgtctct ggcgcggttg
      601 gtgggggcga acaaggccta catcgagaag gagatcccgc acatctcgcg gctccttgcg
      661 aacgatctgg ccgaggtagc ggcgttcgcc gacgtgctcg tggtcgggca ccgctcgccg
      721 gacttcgccg acgcggcgcg ccgcgcgtcg tccgggaaga aagtggtcgt cgacctcgcg
      781 cgcgtgttcg acgcgccccc cgacgaccgg agctatctgg gattcaattg gtaaggaagc
      841 gcgcgagcta ttccgaaagc cgcgcgtccg agctgcggcc gcccgggaat acgctcgcga
      901 agccgtcgtt gtagcgcatg atgagatacg cgccggccaa gagagcgatg ttccggacga
      961 tcaagagcca tgatgtcgat gagccgccct cggagccgaa gcagccgcag tcgacagtca
     1021 tgccgcgcac gatcaccatc gagatggcga cgatgaacat gacgagcagg atggcgatgg
     1081 cacccgcagc cttgcgcgta aagatgccga gaatcagcat gagacccgcg atcagttcgg
     1141 tccacgagac cccgagcgcg aacaagttcg acagcgcgaa cggaatgatc ttgtacccgc
     1201 gcaccgacat cgcgaaaccc gcgggctgga gcaccttgtc ccacgccgcg tataggaaca
     1261 tcccgcccag gaacaggcgc agcaccagat tcaattcgcg gccggtgagg atgcgggcga
     1321 tcacttgtcg accatgcctt cgatggggta gccggccgtg gtccactggt cccagccgcc
     1381 cgagaacacc gacacgttct ggtagccgat gatcttgagc tcggttgcga gctggtcgct
     1441 gaagtcgcac gtgaggcttc ggcaatagac gacgacgtgc gcgtccatag gaacctggga
     1501 attcaagatg tcgaaatact cgggaatgcg gtcgtacgga atgttgatag cgccggggat
     1561 gtgcccttgc tcgtactcgg cggcgtcacg agcgtcgagt atgatggccg acccctcgtc
     1621 gaacaggcgc ttcatctcgg cgagcgagat ggcgccttcc gcgagcgcgt gggtggtatc
     1681 ggcggcggca gagtcggcgt gcaccgcggg gtgctgtgcg gtcgataccg gcgcccccga
     1741 ctggatcaac gcgacgccgt tggggcgcac cgcgttgacg accacgccca tgagggtcgc
     1801 gacgaccatg acgcccaaac ccccgaccat ctgcgataac gcgtttttca ttcgatctgg
     1861 aaatcccccg gaacgacccg tggaaacgca tcctagcgga cgcggggcgg acaatccagc
     1921 cgtttctaac accggccacg ggccgaaggt tcccgggatt ctttgggagg cagatgggtg
     1981 gatgggaggc gagcgctagc gatcgcgcgg atcccgcgtc atagcgtctt tgattcgctg
     2041 cttgcgctcg tccgggagtt cgcccggaca attgtgctcg gcacgcaggc ccgcttc
//
LOCUS       DASPGK010000267         9992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_184697, whole genome shotgun sequence.
ACCESSION   DASPGK010000267 DASPGK010000000
VERSION     DASPGK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184697"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VEC56_07245"
     CDS             <1..762
                     /locus_tag="VEC56_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV13986.1"
                     /translation="EDVPGFDRGGVYLCGNDVAESTIASSSPSAAALRSTYIPFSLTT
                     GNHRPAYGVSPLVETVPGGLLDSWGLGDFYLYGDGDFDVMTPLGTTVVAATYGPAVAN
                     NAAVIANSVGNARVVLSGFSLISLRDDEPSFDRGRFQRNILQYLADFFFLPIVDVSPM
                     RATRLEQNYPNPFNPATTIAFSLAEHGRVRVDVFTVSGQRVKTLVDEERGAGSYTDVR
                     WDGTDAGGRPAASGVYFYRLVAGDFSQTRKMVLLK"
     gene            complement(818..894)
                     /locus_tag="VEC56_07250"
     tRNA            complement(818..894)
                     /locus_tag="VEC56_07250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(858..860),aa:Pro,seq:ggg)
     gene            complement(944..1339)
                     /locus_tag="VEC56_07255"
     CDS             complement(944..1339)
                     /locus_tag="VEC56_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXV13987.1"
                     /translation="MRYKTTTGKMYYTIGEVSEITAVKPHVLRYWETEFPTLRPKKNR
                     AGNRSYRPRDIKSVLAIRELLYKEKFTINGARKKLQENFGNPDPILKQMQIPFSDPQA
                     RHVLLSVKKELEELRELLSEEIEDEVAKV"
     gene            complement(1336..2376)
                     /locus_tag="VEC56_07260"
     CDS             complement(1336..2376)
                     /locus_tag="VEC56_07260"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXV13988.1"
                     /translation="MRVAVLGAGSWGTSLAIVLAENGHDVALWEFDAEHATTVARKRE
                     NTKFLPGVTLPASIDVTNDLGGALRDARMVVFVVPSHAIRATAHAVRAASTFDPEALV
                     ACAAKGLEEGTLARMSEVLREELRLEPGRIVSLVGPSHAEEVSRRIPTSIVAAGSDAA
                     ACAQVQDAFVRPYFRVYTNDDVVGAEIATSLKNSIAIAAGILDGLGLGDNTKAALVTR
                     GLAEITRLGVAMGASAETFSGLAGVGDLVVTCLSRHSRNRHVGEALGRGETLEQVLAG
                     MVMVAEGVRTTRAAVELGRRHKVELPIIEMVHRVLFEGHDPKAAVTELMMRPPRAEAR
                     GETSGRAKGGRG"
     gene            complement(2379..2999)
                     /gene="plsY"
                     /locus_tag="VEC56_07265"
     CDS             complement(2379..2999)
                     /gene="plsY"
                     /locus_tag="VEC56_07265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXV13989.1"
                     /translation="MSILLAALGAYLVGGIPFSYIAGKLAKGIDLRQHGSGNLGASNT
                     YRILGGRIALVVLILDVAKGLVPVVVAARVDPYGGEPVHAIAAAAGAILGHLFSPYLK
                     FSGGKGIATSAGAFAGLAPWAFVCALFVFAVVFAARRIVALASLAAAVTLPLAMFVAP
                     GFGLGDRHASLQWVTILVMLVVVWKHRGNIRRLRAGTEPALARRKG"
     gene            complement(2999..4408)
                     /gene="der"
                     /locus_tag="VEC56_07270"
     CDS             complement(2999..4408)
                     /gene="der"
                     /locus_tag="VEC56_07270"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXV13990.1"
                     /translation="MKKKASVAIMGRPNVGKSTLFNRVTSARRALVHKTPGVTRDAQR
                     GTTEWNGVAFDLVDTGGLFSGIADPLVAQVEERAMREALGADVILLVVDGEAGLTPAD
                     ADVAKRARDTNLPVLLVVNKTERQVSRLGAGEFHKLGFDNLYEISALHGEGIGDLLDD
                     VVKLLPRSSAGAVHTDLRLAIVGVPNVGKSSLVNAIVGSDASIVDDRPGTTRDSIDSA
                     VMWQKRKVTLVDTAGIKRKARTTEDLDLLSTMKSLESIERCDVAVVMLDATRDISNQD
                     VKVASYPHRAGKGVVVCFNKWDIVPKKGDKTYVEKEREFRKKCGFLNYAPVIFISAKT
                     HQRVNVVLETAWRVREECHRKVPTSDFNRFLTRSTQRTPPPYHAGGNGKIFYGTQVDV
                     APPTFNLFVNRVAHFGRYYMRQLSNQLRSEYGFEGTDIRIELAQRRDGKAALRKEDKP
                     RATRRARLKKSSRRKRAAS"
     gene            complement(4410..5735)
                     /locus_tag="VEC56_07275"
     CDS             complement(4410..5735)
                     /locus_tag="VEC56_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HXV13991.1"
                     /translation="MAERALKIVSMKDPADFGLAPGVLAVGDAVVEVAGKPAEDQLDF
                     HFHASRGKSVTILVERANGTRERIVLPTSAVAGLEIWFEAMDFRRCRCKCPFCFVDQM
                     PKGMRETLYVKDEDFRLSFLYGNFTTMNDVTDDEIAKIVEQRNSPQWVSVHVIDEAMR
                     RFIFGRPMKRDIARTLRTLSEGGITVHTQAVIVPGRNDGDYLRETIETLEGIGANIAT
                     LAVVPVGLTRHREGLSSIRSYRDEEMAPVIDLVESYQARFLEKRRSRFVFASDEWYVG
                     AGRVAPGYDTYEDFPQLDNGVGSIRNFLAEIEADLDAFELPADASSIRIATGKLAARV
                     FERYVFPLLTARGLRTLPEVTVVENEYFGAGVTCSGLLVGRDIVAAVSKAGSAKTTFI
                     PPNCLNYDGVTIDEMSVDDLARETGAPVVAPDESFVEALCEHASSGSTK"
     gene            complement(5741..6460)
                     /locus_tag="VEC56_07280"
     CDS             complement(5741..6460)
                     /locus_tag="VEC56_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV13992.1"
                     /translation="MRRALRTTGLRIAALLLIAAAAGALSCASAPPAGSRHDLDHAVD
                     AFIDGDYRGAVTKLDAVIAASTDDAVRREAYTYLGRAHMALGETEAAVDAFARGVQLG
                     DTGPCVAYLELLKQYVEGKPSGLHMTDVLTRGQLAGAIVRLFLGGASSDPSGPTPITE
                     LARRGWMPALADGHDHADLPVTYASLYVVTARILADAGHERQADHIMPGGYRAASRKT
                     EPVSGAEGLAVLERVRALTAK"
     gene            complement(6475..6550)
                     /locus_tag="VEC56_07285"
     tRNA            complement(6475..6550)
                     /locus_tag="VEC56_07285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6515..6517),aa:Gly,seq:ccc)
     gene            complement(6601..7554)
                     /gene="miaA"
                     /locus_tag="VEC56_07290"
     CDS             complement(6601..7554)
                     /gene="miaA"
                     /locus_tag="VEC56_07290"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXV13993.1"
                     /translation="MPTPIRAIAIMGATATGKSALAMSLALDFGGEIVSMDSRQVYRG
                     FDVGTGKVSRDERAPVPHHLIDVADPDQWWSAGRHAALAERAVREIAGRDRVPLLVGG
                     TGLYFRALFGGLVDVVIPADELARIRAGFAGQETRALYDHLLHEDPARARELSVNDRV
                     RITRALELLLYTGVRPSELYARQREAGDDVAYLKLVLSMPRERLRQRIAERTRALFAA
                     GWPAEVERLLASGVSSEAPAMNGLGYRAIARAVDAGVDPASCLDDVVQETQQYAKRQE
                     TFFRSEKDAVWVDVSEAGWEAGVEARVGAFVGLPPGRSKAQ"
     gene            complement(7588..9468)
                     /gene="mutL"
                     /locus_tag="VEC56_07295"
     CDS             complement(7588..9468)
                     /gene="mutL"
                     /locus_tag="VEC56_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXV13994.1"
                     /translation="MPVIRILPDDVARRIAAGEVVERPASVVKELFENAVDAGAKSIT
                     VTVSGGGDALIEIRDDGTGMAREDVELAPRNFATSKIHDADDLHRIASYGFRGEALAS
                     ISSVSRFEIVSSAATTGEGWRIKVEGKSIVESAPAPHARGTTVRVRDLFFNTPARKRF
                     LKSSITERRRILETILSFALILPEVEIHYIDDGRHVLDFLPARSWRERVAAVLGASVM
                     RHMVDVDAQSGALRVQGFVSLPSHTRGNRFSQFIFVNQRAVRERTLVHALQDAYRNVI
                     PYKRYPVAVLSLTMPPGEVDVNVHPSKLEVRVQNERAVFAAVRQAIKTALSSKSENVL
                     AVARAARAADDAGVVSPRAGAWATSSGETRHAGSQARVPAGVPGLQGLEGAVSSNDRI
                     RDAFADYVARRASAAPAPEEAKAAELALGTVEAPAPPETAPQVAPADDALYWQFNHTF
                     IFIQVRGGVVVIDQHAAHERILFDRGMRQLEGSSPPSQQILFSIPIELSLRELEVFRS
                     SKDVFNRLGFTLEPFGGTSILVRGYPQGIKNWSEGRLLLQIFDDILADRAPGDTQTEK
                     LVASYACRSAIKAGQKLSVDEMKLLADQLFAADHPYSCPHGRPTIHRLSVDEVARWFH
                     RR"
     gene            complement(9575..>9992)
                     /locus_tag="VEC56_07300"
     CDS             complement(9575..>9992)
                     /locus_tag="VEC56_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV13995.1"
                     /translation="LAKMGLVPQGDMTWQERIQSEFVEASGRLYAGQSSTFAAVMARE
                     GSFELVFLSELGDGTWLATGFADEIDRELVTVSADGHVLTHPRVRIENRDEPVAKIAA
                     AHQAKLRKPPAPPKAAPTDPASVRGAFDRFLTAVYG"
BASE COUNT         1606 a         3425 c         3281 g         1680 t
ORIGIN      
        1 gaagacgtcc ccgggttcga tcgcggcggc gtttacctgt gcggcaacga cgtggccgag
       61 agcacgatcg catcgagcag cccttccgca gcggccctcc gctcgactta cattccgttt
      121 tcgttgacga cgggcaacca ccgcccggcg tacggcgtta gtccgctcgt cgagacagtg
      181 ccgggcgggc tgctcgacag ttggggtctg ggcgatttct atctctacgg cgacggcgac
      241 ttcgacgtca tgaccccact cggcacgacc gtcgtcgcgg cgacctacgg tccggccgtc
      301 gccaacaacg cggcggtcat cgcaaactcc gtcgggaacg cccgtgtggt tctttccggc
      361 ttcagcctga tcagcctccg tgacgacgag ccctccttcg accgcggtcg attccaacgc
      421 aatatattgc agtacctcgc ggacttcttt tttcttccga tcgtcgacgt ctcgcccatg
      481 cgggcgacgc gcctcgagca gaactatccc aaccctttca accccgcgac cacgatcgcc
      541 ttctcactag ctgagcacgg ccgcgtgcgc gtcgacgtgt tcacggtctc cgggcagcgc
      601 gtgaagacac tcgtcgacga ggagcgcggg gcggggtcgt acaccgacgt ccgctgggac
      661 ggcacggacg cgggtggtcg gcccgcggca agcggcgtgt acttctaccg gctcgtcgcc
      721 ggagacttct cgcagacgcg gaagatggtg ctgctcaagt agcggataga aacgaaaaac
      781 gggggccgag tgactcggcc cccgttctca agaaaaatgg tcggggcgac tggattcgaa
      841 ccagcgacca cttgcacccc atgcaagtgc gctaccggac tgcgccacgc cccgaccaga
      901 cggctagact aacagagacg cgggcgtgcg cgcaaccgtc gcgtcacacc ttggcgactt
      961 cgtcctcgat ctcctcggac aacaactcgc gcaactcctc caactctttc ttgacgctca
     1021 gcagcacgtg gcgcgcctgc gggtcgctga aggggatctg catctgcttc aggatgggat
     1081 ccggatttcc gaagttctct tgcagcttct tgcgcgcgcc gttgatggtg aatttctctt
     1141 tgtacagcag ttctcgaatg gccaggacgg atttgatgtc ccgcggccgg tagctgcggt
     1201 tgccggcccg gttcttcttg ggccgcaacg tgggaaactc ggtctcccag taccgcaaca
     1261 cgtggggctt taccgcggtg atctcggata cttcgccgat cgtgtaatac atcttgcccg
     1321 tcgtcgtctt gtacctcacc cgcgtcctcc tttcgcgcgt ccggaggtct cgccgcgagc
     1381 ctccgcccgc ggcgggcgca tcatgagctc ggtcacggct gccttggggt cgtgcccttc
     1441 gaagagcacc cgatgcacca tctcgattat cgggagctcg actttgtgac gccgccccaa
     1501 ctcgacggcg gcgcgcgtcg tgcgcacgcc ctccgccacc atgaccatgc ccgcgagcac
     1561 ctgctccaac gtctcgccgc gtccgagcgc ctcccccacg tgccggttgc ggctgtggcg
     1621 actcaaacag gtaaccacga ggtcgcccac cccggcaaga ccggaaaagg tctcggccga
     1681 cgcccccatg gccactccca gtcgtgtgat ctcggccagt cccctcgtca ccagggccgc
     1741 cttggtgttg tcccccaggc ccaggccgtc gagaatcccg gccgcgatcg caatcgagtt
     1801 cttgagcgac gtcgcgatct cggccccgac cacgtcgtcg ttggtgtaaa cccgaaaata
     1861 cggccgaacg aacgcgtcct ggacctgggc gcacgcggcc gcgtcggagc cggccgcgac
     1921 gatcgaggtg ggaatgcggc ggctcacttc ctcggcgtga ctcgggccta ccaggctcac
     1981 gatgcggccc ggctcgaggc gcagttcttc gcgcaggacc tcgctcatgc gcgcgagggt
     2041 cccctcttcg agacccttgg ccgcgcatgc gaccagcgct tccgggtcga aggtgctcgc
     2101 ggcccgcacc gcgtgcgcgg tcgcccgaat ggcgtgcgag gggaccacga aaacgaccat
     2161 gcgcgcatcg cgaagggcgc cccccagatc gttcgtcacg tcgatactcg ccgggagcgt
     2221 gacgccgggc aggaacttcg tgttctctct cttgcgcgcg acggtcgtcg cgtgttccgc
     2281 gtcgaactcc cacagcgcga cgtcgtggcc gttctcggcc aggacgatcg cgagggaggt
     2341 gccccagctt ccggctccca ggacggcgac gcgcatcgtt accctttccg ccgcgcgagc
     2401 gcgggctcgg tcccggcgcg caagcgccgg atgttgcctc gatgcttcca gaccacgacc
     2461 agcatgacca ggatggtcac ccattgcagc gacgcgtgcc ggtctcccag tccgaagccg
     2521 ggggcgacga acatcgccag gggcagcgtg accgcggccg cgaggctcgc gagcgccacg
     2581 atgcgccgcg ccgcgaacac caccgcgaac acgaacaggg cgcacacgaa cgcccacggc
     2641 gccagcccgg cgaacgcacc cgcggaggtc gcgatgccct tcccgcccga gaacttcaaa
     2701 tacggagaga acaggtggcc gaggatcgcc ccggccgcgg cggcgatggc gtggaccggc
     2761 tccccgccgt aggggtcgac gcgcgccgcc accaccaccg gcaccagccc cttcgcgacg
     2821 tcgagtatca ggacgaccag cgcgatgcgt ccgccgagaa tccgataggt gttcgaggcc
     2881 cccaggttgc cggagccgtg ctgtcgcagg tcgatcccct tcgcgagctt ccccgcgatg
     2941 tagctgaacg ggatcccgcc caccaaatac gcgcccaacg cggccaggag aatcgacatc
     3001 acgacgccgc tcgcttcctg cggctgctct tcttcaaacg ggcgcggcgc gtcgcgcgcg
     3061 gcttgtcttc cttgcgcaag gccgccttgc cgtcgcggcg ctgcgccaac tcgatgcgga
     3121 tgtcggtgcc ttcgaagccg tactcggagc gcaattggtt gctcaactgg cgcatgtagt
     3181 agcgcccgaa gtgggccacg cgattgacga acaggttgaa ggtgggcggt gcgacgtcca
     3241 cttgcgtgcc gtagaagatc ttcccattgc cgccggcgtg gtacgggggc ggcgtgcgct
     3301 gggtcgagcg ggtgaggaag cgattgaaat cggaggtcgg caccttgcgg tggcattcct
     3361 cgcgcacgcg ccacgcggtc tccagcacca cgttcacgcg ctgatgcgtc ttggccgata
     3421 tgaaaatgac cggcgcgtag ttcaagaacc cgcacttctt gcggaattcg cgctccttct
     3481 cgacgtaggt cttgtcgcct tttttgggta cgatgtccca cttgttgaaa cacaccacca
     3541 cacccttgcc ggcgcggtgc ggatagctcg ccaccttgac gtcctggttg gagatgtcgc
     3601 gggtggcgtc caacatcacg accgcgacgt cgcagcgctc gatcgattcg aggctcttca
     3661 tggtgctcaa caggtcgagg tcttcggtgg tgcgggcctt gcgcttgatg ccggcggtgt
     3721 cgaccagcgt caccttcctc ttctgccaca tgacggccga gtcgatggag tcgcgcgtgg
     3781 tgccggggcg gtcgtcgacg atgctggcgt cggagccgac gatggcgttg acgaggctcg
     3841 acttccccac gttgggaaca cccacgatgg cgaggcgcag gtcggtgtgc acggcgcccg
     3901 ccgacgagcg cggcagaagc ttcacgacgt cgtccaacag atcgccgatg ccttccccgt
     3961 ggagcgcgct gatctcgtag aggttgtcga aaccgagctt gtggaactcg cccgcaccga
     4021 ggcggctcac ctggcgttcg gtcttgttga ccaccagcaa cacgggcagg ttggtgtcgc
     4081 gcgcgcgctt ggccacgtcg gcgtcggccg gcgtgagccc cgcttccccg tccaccacca
     4141 gtagaatgac gtccgcgccc aaggcttcgc gcatcgcgcg ctcttccact tgggccacca
     4201 acgggtccgc gatgccggaa aacaggccac ccgtgtccac gaggtcgaac gcgacgccgt
     4261 tccactccgt ggtcccgcgc tgtgcgtcgc gcgtgacgcc gggcgttttg tgcaccaagg
     4321 cgcggcgcgc cgacgtcacg cggttgaaga gcgtcgactt gccgacgttc gggcggccca
     4381 tgatcgcgac gctggctttc ttcttcatgc tacttcgtcg accccgacga cgcgtgctcg
     4441 cacagcgcct cgacgaacga ctcgtcgggc gcaaccaccg gggcgccggt ctcgcgcgcg
     4501 aggtcgtcga ccgacatctc gtcgatggtg acgccgtcgt agttcaaaca gttgggcggg
     4561 atgaaggtgg tcttggccga acccgccttc gacaccgccg ctacgatatc gcggccgacg
     4621 agcagacccg agcacgtgac ccccgcgccg aaatactcgt tctcaaccac cgtgacttcc
     4681 ggcagcgtgc gcaggccgcg cgcggtcaac aatggaaata cgtagcgttc gaagacgcgc
     4741 gcggccaatt tcccagtggc gatgcgaatc gagctcgcgt cggccgggag ctcgaaggca
     4801 tccaagtcgg cttcgatctc ggccaggaag ttgcgaatcg acccgacacc gttgtcgagc
     4861 tgtgggaagt cctcgtaggt gtcatagccg ggcgcgacgc gcccggcgcc gacgtaccac
     4921 tcatcgctgg caaatacaaa acgagagcgt cgcttttcga gaaaacgggc ctggtacgat
     4981 tccaccaagt cgatcaccgg cgccatctcc tcgtcccgat acgatcgaat gctcgaaagg
     5041 ccctcgcggt gtctggtgag tccaaccgga accaccgcca gagttgcgat gttggcgccg
     5101 atcccttcca gcgtctcgat ggtctcgcgc aggtagtcgc cgtcgttgcg tcccggcacg
     5161 atgacggcct gtgtgtgcac cgtgatgccg ccctcggaca acgtgcgcaa ggtccgcgcg
     5221 atgtcgcgct tcatcgggcg cccgaagatg aagcgacgca tggcctcgtc gatcacgtgc
     5281 accgacaccc actgcggcga gttgcgctgt tcgacgatct tcgcgatctc gtcgtccgtc
     5341 acgtcgttca tggtggtgaa gtttccgtac aggaagctca agcggaagtc ttcgtccttg
     5401 acgtagagcg tctcgcgcat gcccttgggc atttggtcga cgaagcagaa cgggcacttg
     5461 cagcggcagc ggcggaagtc catggcctcg aaccagatct ccaggcccgc caccgcgctg
     5521 gtcggcagca cgatgcgctc gcgcgtcccg ttggcgcgct cgaccaggat cgtgaccgac
     5581 ttcccgcgcg aggcgtggaa gtggaaatcg agctggtcct cggccggctt gccggccact
     5641 tcgacgaccg cgtcgcccac ggcgagaacg cccggcgcca acccgaagtc ggcggggtct
     5701 ttcatcgaga cgatcttgag agcacgttcg gccatagttc ttacttcgct gtcagcgcgc
     5761 gcacgcgctc gagtaccgcg agtccttccg cgccggacac cggctcggtc ttgcgcgacg
     5821 ccgcgcggta tccgcccggc atgatgtgat cggcctgccg ctcgtggccc gcgtccgcga
     5881 gaatgcgggc ggtgacgacg tacagcgagg cgtacgtgac gggaaggtcg gcgtggtcgt
     5941 gtccgtcggc gagtgccggc atccacccgc gccgcgcgag ctccgtgatg ggcgtggggc
     6001 ccgacgggtc gctcgaagcg ccgcccaaga acaagcgcac aatcgcgccc gcgagctgcc
     6061 cgcgggtcag cacgtcggtc atgtggaggc cggacggctt cccttcgacg tattgcttga
     6121 gcagctccaa gtacgccacg cacggtcccg tgtcgcccag ctgcacgccg cgcgcgaacg
     6181 cgtcgacggc ggcctcggtc tcgcccagcg ccatgtgcgc acgacccagg tacgtgtacg
     6241 cctcgcgtcg cacggcgtcg tcggtggagg ccgcgatgac tgcgtcgagc ttcgtcaccg
     6301 cgccgcggta gtccccgtcg atgaacgcgt cgacggcgtg gtccaggtcg tggcgggacc
     6361 ccgccggggg tgccgaggcg cacgagagcg ccccggctgc cgctgctatc aagagaagcg
     6421 ccgcgattcg aaggccggta gtgcgcagcg ctcgacgcat gggatcaagg ggaatggagc
     6481 gggcaacggg gctcgaaccc gcgaccttca gcttgggaag ctgatactct accaactgag
     6541 ttatgcccgc tctcctaacg tgctgtgata aaagcatctt agacatatcg tgtcaagttg
     6601 ttattgagcc ttgctccggc ccggcggcaa gcccacaaag gcgccgacgc gcgcctcgac
     6661 cccggcctcc caacccgctt ccgagacgtc gacccagacg gcgtccttct cgctccgaaa
     6721 gaaggtctcc tggcgtttgg cgtactgctg cgtttcctgg acgacgtcgt cgagacacga
     6781 cgcggggtcg accccggcgt cgacggcccg cgcgatcgcg cggtagccca ggccgttcat
     6841 cgccggcgcc tccgaggata cacccgacgc gaggaggcgc tccacttcgg cgggccaccc
     6901 cgccgcgaag agcgcgcgcg tgcgctcggc gatgcgttgg cgcaggcgct cccgcggcat
     6961 cgacagcacg agcttcaggt acgctacgtc gtcacccgct tcccgctgac gcgcatacaa
     7021 ctcgctcggc cgcaccccgg tgtagagcaa cagctccagc gcccgcgtga tgcgcacgcg
     7081 gtcgttgacc gagagctcgc gggcgcgcgc ggggtcctcg tgcagcaaat gatcgtacag
     7141 ggcgcgcgtc tcttgaccgg caaagccggc gcggatgcgc gcgagctcgt cggcgggaat
     7201 cacgacgtcg accagtccgc cgaagagcgc gcgaaagtag aggccggttc cgcccaccag
     7261 caggggcacg cgatcgcgcc ccgcgatctc tcgcaccgcg cgctcggcga gcgcggcgtg
     7321 acgccccgcg ctccaccatt ggtccgggtc ggcgacgtcg atcaggtggt gcggcacggg
     7381 cgcacgctcg tcgcgcgaga ctttcccggt cccgacgtcg aagccgcggt acacttgacg
     7441 cgaatccatg gagacgatct cgccgccgaa gtcgagcgcc agcgacatcg ccagcgccga
     7501 tttgccggtg gccgtcgccc ccatgatggc aatcgctcga atgggtgtgg gcatgtgcac
     7561 cgtgcccggg ttcgccgcga ggcggcccta gcgccggtgg aaccagcgcg cgacttcgtc
     7621 gactgagaga cggtggatgg tcggccggcc gtgcggacac gaatacgggt ggtcggccgc
     7681 gaacaactgg tcggcgagca gcttcatttc gtcgaccgag agcttctgcc ccgccttgat
     7741 ggcgctgcgg cacgcgtagc tcgccaccag tttctcggtc tgcgtgtcgc cgggcgcgcg
     7801 gtcggccagg atgtcgtcga agatctgcag caagagccgc ccttcggacc aattcttgat
     7861 gccctgcggg tagccgcgca cgaggatgct ggtcccgccg aagggttcca gcgtgaagcc
     7921 gagacgattg aagacgtcct tcgaggatcg aaacacctcg agctcgcgca gagacagctc
     7981 gatcggaatg ctgaacagaa tctgctgcga cggcggcgac gagccttcca gttggcgcat
     8041 gccgcggtcg aagaggatgc gctcgtgggc ggcgtgctgg tcgatgacga ccacgccgcc
     8101 gcgcacttga atgaagatga acgtgtggtt gaactgccaa tacagcgcgt cgtccgcggg
     8161 tgcgacctgc ggtgccgtct ccggcggtgc gggcgcttcg accgtgccga gcgcgagctc
     8221 ggccgccttc gcctcctcgg gggccggggc cgccgacgcg cgccgcgcga cgtagtcggc
     8281 gaaagcgtcg cggatgcgat cgttcgaact caccgctcct tccaagccct gcagaccggg
     8341 tacgccggcg ggaacgcgcg cttgcgaacc cgcgtgccgg gtttcgcccg acgacgtggc
     8401 ccacgcaccc gcgcgcggag ataccacgcc tgcgtcgtcc gccgcgcgcg cggcccgcgc
     8461 caccgccagc acgttctcgc tcttggacga aagcgcggtc ttgatggcct gccgaaccgc
     8521 ggcgaacacc gctcgctcat tctggacgcg cacctcgagc ttgctcgggt ggacgttgac
     8581 gtcgacctcg ccgggcggca tggtcagcga gagcacggcc accgggtaac gcttgtacgg
     8641 aatcacgttt cgatacgcgt cctggagcgc gtgcaccagc gtgcgctcgc gcaccgcgcg
     8701 ctggttgacg aagatgaact ggctgaagcg attgccgcgc gtgtgcgagg gcagactcac
     8761 gaagccctgg acgcggagcg ctcccgactg cgcgtcgacg tcgaccatgt ggcgcatcac
     8821 actcgccccc aagacggcgg cgacgcgctc gcgccagctg cgcgcgggga ggaagtccag
     8881 gacgtgacgg ccgtcgtcga tgtaatggat ttcgacctcg ggcaggatca acgcaaacga
     8941 gagaatcgtc tccaggatgc ggcgccgttc ggtgatcgac gacttcaaga agcgcttgcg
     9001 cgcgggcgta ttgaagaaga gatcgcgcac gcgcaccgtc gttccgcgcg cgtgcggcgc
     9061 gggagcactc tcgacgatcg acttcccttc caccttgatg cgccagcctt cgcctgtcgt
     9121 ggccgcgctg gacacgatct cgaagcgcga aaccgacgag atgctggcca gggcctcgcc
     9181 gcggaagccg tagctcgcga tgcgatggag gtcgtcggcg tcgtggatct tgctggtcgc
     9241 gaagttgcga ggcgccagct cgacgtcctc gcgcgccatg ccggtgccgt cgtcgcgaat
     9301 ctcgatcagc gcgtcgccgc cgccagacac ggtcacggtg atcgatttcg cccccgcgtc
     9361 gaccgcgttc tcgaagagct ccttgaccac cgaggccggc cgctcgacca cttctccggc
     9421 cgcgatccgg cgcgctacat cgtctggaag tatgcgaatg acaggcattt agcgtctccg
     9481 ttgcaggtat cgccagacac cttagcacgg agcgcgcggc acggcaacga gactgtcgcg
     9541 ggcgcggctg gaattgtggg gataaccggc gcgtctaccc gtagaccgcc gtcaggaacc
     9601 ggtcgaaggc gccgcggacc gacgccgggt cggtcggcgc ggccttcggc ggcgcgggcg
     9661 gcttgcgcag cttcgcctgg tgggccgccg cgatcttcgc caccggctcg tcccggttct
     9721 cgatccggac gcgcgggtga gtaaggacgt gtccgtcggc gctcaccgtc acgagctcgc
     9781 ggtcgatctc gtcggcgaac ccggtggcga gccaggtgcc gtcgccgagt tccgagagga
     9841 aaacgagctc gaaggagcct tcgcgcgcca tgacggccgc gaaggtgctg gactggcccg
     9901 cgtagagccg gccgctcgcc tcgacgaact cgctttgtat gcgctcctgc cacgtcatgt
     9961 cgccttgcgg cacgagtccc atcttggcga gc
//
LOCUS       DASPGK010000268         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_184796, whole genome shotgun sequence.
ACCESSION   DASPGK010000268 DASPGK010000000
VERSION     DASPGK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184796"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VEC56_07305"
     CDS             complement(<1..783)
                     /locus_tag="VEC56_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXV13996.1"
                     /translation="MISPRVDRVVREGVLASLATAVVAAALGALWLFATRVASDPPAS
                     RFVVWTLAGGVATAVLAVAMSRLFGRGSERLTSLGLPNALTLARLVLIAPTVVLLLDA
                     RYPEALVCYFVLFATDVADGIVARRRGTVSEFGVVADPLADVLSTFAIFTVFLVRGWC
                     PLWLYALLSARYAMLFVGSFFLFLASGPFTFRATVPGKIVGVVQAFGACLVIGGAMAG
                     GLDPAIEKVLFAFLGIGFASIVLSQAVIGWRLARRTVSPSGPT"
     gene            complement(780..3353)
                     /gene="ppdK"
                     /locus_tag="VEC56_07310"
     CDS             complement(780..3353)
                     /gene="ppdK"
                     /locus_tag="VEC56_07310"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXV13997.1"
                     /translation="MRDLLGGKGANVAEMANLGVPVPPGFTITTEACAHFMGHAGGYP
                     PGLHDEVAEHLHLLEKESGKQFGSIENPLLVSVRSGARASMPGMMDTVLNLGLNDETV
                     RGLAARTGNARFAFDSYRRFIQMYADVVMGVPSGLFEEVLAKKRVALGVSADNQLDAS
                     ALAELVDAFREIVRDASGVAFPHDPDAQLWGAVAAVFKSWNSPRAVSYRAMYHYPDHW
                     GTAVNVQAMVFGNMGDDCATGVAFTRDPSSGEKRFFGEYLVNAQGEDVVAGIRTPLPV
                     DGSPESLEARMPAIYRELVEVAGKLEHHFTDVQDVEFTVESGRLWMLQTRNAKRTGRA
                     AVRIAVDMVEEGLIDVRTAMKRVAPEQIDQLLHPTIDPERAPEPIAKGLPASPGAASG
                     RVVFAVERAVEMAKEGEAVVLVRNETSPEDIDGMKVARAILTATGGMSSHAALVARGM
                     GKCCVVGCSALQVDYGAREFRVNGRAFREGDEITVEGSTGKVYAGRIPTMRAELDRTF
                     ERFMEWVDSVRRLGVRANADTPEDAVTARRFGAEGIGLCRTEHMFFREDRIDWVRRMI
                     LAATREERVAALEKILPMQREDFAGVFKAMDGYPVTIRLLDPPLHEFVPTQPDEVEAL
                     SRRIGVDVAVLEAKIRALHEFNPMLGHRGCRLGITFPEVYEMQVRAIIEAACAMQKDG
                     VDVRPEIMIPLVGLKAELARMRELVVKEADRVLASKRAKIDYTVGTMIEVPRAALTAG
                     EIATVADFFSFGTNDLTQMTFGFSRDDAAKFLVHYVEEGILPRDPFETIDVDGVGRLV
                     RLATDEGRRAKPELKVGVCGEHGGDPASVRFMHDAGLDYVSCSPYRVPVARLAAAQAA
                     L"
     gene            complement(3436..>4400)
                     /locus_tag="VEC56_07315"
     CDS             complement(3436..>4400)
                     /locus_tag="VEC56_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV13998.1"
                     /translation="TPMSAYNLVQACEAAGLPPGVVNLVTGDGVGVGGRLVEHPDVRV
                     ISFTGSSAVGRRIAESCGRTLKRVALELGGKNAQIVMDDADLDLAIDGVLWGAFGTAG
                     QRCTATSRLILHKPIKKKVLDMLVERVKTLKLGNGLDESVQVGPMVSKVQREGVHRYV
                     EIGKNEDRARLVCGGEFYAKGECEKGWFYAPTIFDSVTRTMRIAREEIFGPVLAVIEA
                     NDLDDAISILNDTPYGLSSSIYTRDVNQAFRAIRDIEAGITYVNGPTIGAEVQLPFGG
                     VKDTGNGHREASHTVLDIFTEWKSVYVDYSGRLQRAQIDTESAE"
BASE COUNT          742 a         1514 c         1468 g          676 t
ORIGIN      
        1 cgtaggtcca ctgggcgaca ccgtccgccg tgccaacctc caaccgatga ctgcttgaga
       61 aagcactata gatgcaaatc cgataccgag aaaagcaaat agcaccttct caatcgcggg
      121 gtcgagcccg cccgccatgg ctcccccgat gacgaggcag gcgccgaagg cctggaccac
      181 cccgacgatc ttgccgggca ccgtggcccg gaacgtaaag ggcccgctgg ccaggaatag
      241 gaaaaaagat cccacgaaca gcatggcgta gcgggccgag agcagggcgt atagccaaag
      301 tgggcaccat ccccgcacga gaaagacggt gaagatcgcg aaggtggaga gcacgtcggc
      361 cagggggtcg gcgactacgc cgaactcgct cacggtccca cgccgtcgag cgacgatgcc
      421 atccgccacg tcggtggcga aaagcacgaa ataacacacg agcgcttcgg ggtagcgggc
      481 gtcgagcaag aggacgacgg tgggtgcgat cagcaccagg cgcgcgaggg tgagcgcgtt
      541 gggcagaccg agagacgtga gtcgctcgct cccgcgaccg aacaaccgcg acatcgccac
      601 ggcgagcacg gcggtcgcga ctccacccgc gagcgtccat acgacgaagc gggacgccgg
      661 cggatcactg gcgacgcggg tcgcaaagag ccagagtgcg ccgagcgcgg ccgcgaccac
      721 ggcggtcgcg agcgaagcca gcacgccttc gcgcacgacg cggtcgacgc gaggagaaat
      781 caaagggcgg cttgcgcggc cgccaggcgc gcgaccggca cgcgataggg cgagcagctc
      841 acgtagtcga ggcccgcgtc gtgcatgaaa cggaccgacg cggggtcgcc gccgtgctcg
      901 ccgcacacgc ccaccttgag ctcgggcttg gcgcggcggc cttcgtcggt cgccaggcgc
      961 accaggcgcc ccacgccgtc cacgtcgatg gtctcgaagg ggtcgcgcgg gagaattccc
     1021 tcctcgacgt agtgaaccag gaacttggcc gcgtcgtcgc gggaaaatcc gaacgtcatc
     1081 tgggtgagat cgttggtacc gaaggaaaag aagtccgcca cggtcgcgat ctcgcccgcg
     1141 gtcaatgccg cgcgcggcac ctcgatcatg gtgccgaccg tgtagtcgat cttggcgcgc
     1201 ttggacgcga gcacgcggtc ggcctctttc accaccagct cacgcatgcg cgcgagctcg
     1261 gccttcagtc ccacgagcgg aatcatgatc tcgggcctca cgtccacccc gtccttctgc
     1321 atcgcgcacg ccgcctcgat gatggcccgc acctgcatct cgtacacctc ggggaaggtg
     1381 ataccgagcc ggcagccgcg gtggccgagc atcgggttga actcgtgcag cgcgcgaatc
     1441 ttggcctcga gcaccgcgac gtcgacgccg atgcggcgcg aaagggcctc gacttcgtcg
     1501 ggctgggtgg gcacgaactc gtgcaggggc gggtcgagca atcgaatcgt gacggggtaa
     1561 ccgtccatgg ccttgaacac gcccgcgaag tcctcgcgct gcatgggcag gatcttctcg
     1621 agggccgcca cgcgctcctc gcgggtggcg gcgagaatca tgcggcggac ccagtcgatg
     1681 cggtcctcgc ggaagaacat gtgctcggtg cggcacaggc cgatgccctc cgcgccgaag
     1741 cggcgcgcgg tgacggcgtc ttccggtgtg tcggcgttgg cgcgcacgcc cagacggcgc
     1801 accgaatcga cccattccat gaagcgctcg aaggtgcggt cgagctcggc gcgcatggtc
     1861 gggatgcggc ccgcgtacac cttgcccgtg ctcccttcca ccgtgatctc gtcgccttcc
     1921 cggaacgcgc gtccgttgac tcggaactcg cgcgccccgt agtcgacctg cagggccgag
     1981 cagcccacca cacagcactt ccccatcccg cgcgcgacta gcgcggcgtg cgacgacatc
     2041 ccgccggtgg cggtgagaat ggcgcgcgcg accttcatcc cgtcgatgtc ctcgggcgag
     2101 gtctcgttgc gcaccagcac caccgcttcg ccctccttcg ccatctcgac cgcgcgctcg
     2161 accgcgaaca cgacgcggcc cgaagccgcc ccgggtgagg ccggcaaccc cttcgcgatg
     2221 ggctcggggg cgcgctcggg gtcgatcgtc gggtgcaaca actgatcgat ctgctccggt
     2281 gccacgcgct tcatcgccgt gcgcacgtcg atcagtcctt cttcgaccat gtcgacggcg
     2341 atgcgcacgg ctgcgcgccc cgtgcgcttg gcgttgcggg tctgcagcat ccacagccgt
     2401 cccgattcga cggtgaactc gacgtcttgc acgtcggtga aatggtgctc gagcttgccc
     2461 gccacctcga ccagctcgcg atatatcgcg ggcatgcgcg cttccaggct ctcgggagag
     2521 ccgtcgacgg ggagcggcgt gcgaatcccc gccacgacgt cctcgccctg ggcgttgacc
     2581 aagtactcgc cgaagaaacg cttctcgccc gaagacgggt cgcgggtaaa cgcgaccccg
     2641 gtcgcgcagt cgtcgcccat gttgccgaac accatcgcct gcacgttgac cgcggtgccc
     2701 cagtggtcgg ggtagtggta catggcgcgg taggagaccg cgcgcggcga attccatgac
     2761 ttgaacaccg ccgccaccgc accccacagc tgcgcgtcgg ggtcgtgagg gaacgcgacg
     2821 ccggaggcgt cgcgaacgat ctcgcgaaac gcatccacca gttcggcaag ggcgctcgcg
     2881 tcgagttgat tgtccgccga cacgccgagt gcgacccgct tcttcgcgag cacttcttcg
     2941 aacaggcccg aggggacgcc catcacgacg tcggcgtaca tctggatgaa gcggcgatag
     3001 ctatcgaagg cgaagcgggc gttgccggtg cgcgcggcga gtccccgcac ggtctcgtcg
     3061 ttgagcccca ggttgagcac ggtgtccatc atgcccggca tggacgcgcg cgcgcccgag
     3121 cgaaccgaga ccaacagcgg gttctcgatc gatccgaatt gcttgccgga ttccttctcg
     3181 aggagatgca ggtgctcggc gacttcgtcg tgcaagcccg gggggtatcc ccccgcgtgc
     3241 cccatgaagt gtgcgcacgc ttccgtggtg atcgtgaagc cgggcggcac gggcacaccg
     3301 aggttggcca tctcggcgac gttggcgccc ttgccgccca ggaggtcgcg catcgcggcc
     3361 gcaccctcgg cgcgcggggc gccgaagtgg tacacgtact tcttgctccg aaccgcccgc
     3421 gccgccgtca cggccctact ccgccgactc ggtgtcgatt tgtgcgcgct gcaagcggcc
     3481 gctgtagtcc acgtagaccg acttccactc cgtgaagatg tcgagcacgg tgtgcgacgc
     3541 ctcacggtgg ccgttgccgg tgtccttgac cccgccgaac gggagctgga cctcggcgcc
     3601 gatggtgggg ccgttgacgt aggtgatgcc ggcctcgatg tcgcggatgg cgcggaacgc
     3661 ctggttgacg tcgcgcgtgt agatcgagct cgaaaggccg tagggcgtgt cgttcaagat
     3721 ggagatcgcg tcgtcgaggt cgttggcctc gatcaccgcc agcacgggcc cgaagatctc
     3781 ctcgcgcgcg atgcgcatgg tgcgcgtaac actgtcgaag atggtgggtg cgtagaacca
     3841 gcccttttcg cactcgccct tggcgtagaa ctcgccgccg cacaccaggc gcgcgcggtc
     3901 ctcgttcttc ccgatctcga catagcggtg cacaccttcg cgctgcacct tgctcaccat
     3961 cggccccacc tgcacggact cgtccagccc gttgccgagc ttcagcgtct tgacgcgctc
     4021 gaccagcata tccaggacct tcttcttgat cggcttgtgg aggatcaagc ggctggtcgc
     4081 cgtgcaacgc tggcccgcgg tgccgaaagc gccccacagc acgccgtcga tcgcgaggtc
     4141 caggtcggcg tcgtccatga cgatctgcgc gttcttgccg ccgagttcca gcgcgacgcg
     4201 cttgagcgtg cggccgcacg actccgcgat gcggcgcccg accgccgacg agccggtgaa
     4261 ggagatcacg cgcacgtcgg ggtgctcgac cagccgcccg ccgacgccga caccgtcgcc
     4321 ggtgacgaga ttgacgacac cgggcggaag gcccgcggct tcgcacgcct gcaccagatt
     4381 gtacgccgac atgggcgtgt
//
LOCUS       DASPGK010000269         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_420269, whole genome shotgun sequence.
ACCESSION   DASPGK010000269 DASPGK010000000
VERSION     DASPGK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420269"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2330)
                     /locus_tag="VEC56_07320"
     CDS             complement(<1..2330)
                     /locus_tag="VEC56_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV13999.1"
                     /translation="MTKTLQPEDVIAQYRVVGPLGAGGMGEVYLAQDQSLERNVALKV
                     LPPDLVRSEDRVRRFVQEAKSASSLNHPNIVTIYEIGNEKVKSSSGSESGPLQFISME
                     LVSGKTLGAKIHDERTDLRTLLGYLAQAAEGIAKAHAAGIVHRDLKPGNIMISDDGFA
                     KVLDFGLAKLTEKRLESASATAMTEIADLTSEGAVLGTVHYMSPEQVQAKSVDHRSDI
                     FSFGCLLYEAATRRRPFVAESNVETMHKILNEKPTPVEDLNPQAPAELRRLIRRCLAK
                     APDQRLQSMKDLAIELREIVDEYEALSASATSATTTGKTPPIVARKSRRFSPLVAGAI
                     LIGVLGIGYGIWSAGRRGDQGANPFENMRAVTQTSRGDVNFSALSRDGRYLAYIAGAA
                     GRSTLRVRQVATGSDVEILPLANVAIESPSFSPDGNYLFYLKRLPEQPNYRTLMRVPS
                     LGGSEEQKGFDVDSRVTFSPDGKRVCFMRGVPQESRSALVVLDIDGGAERELANVYRP
                     EVISGVPSWSPDGKRVGVFIIQQVPTARSILIMFDAESGKREDFFVQKDAFFTDLAWM
                     PDGRGLVAAGTVLKDGVSSAVYVITYPRGELRRVTNDFNQYSQVSTSSGSGAIGAVRV
                     SQVTNLFVADPADGSTRRLTSATSPEHSQFGFDLTADRVVFLAAHDRRLGISSIPFEG
                     GEPRRIAVGPGHATSLVVRGDRVVFERYDDDGQQHIWRIKLDGTGLAQLTSGGGENLL
                     DVSRDGTLVTYSRADSTHGVWLVSADGGEPRSLDASA"
     gene            complement(2394..3761)
                     /locus_tag="VEC56_07325"
     CDS             complement(2394..3761)
                     /locus_tag="VEC56_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14000.1"
                     /translation="MNVAVLISILYGDARGYRRRAIRRRVGANQRLSYDELTGLGERE
                     FLGHVYRSIARFPYYAERVKAHRGSIPKPGEPLRPEELPVWTRHDQREFFAQQERPAD
                     SMFAHQTSGSTSLPIRFHITRESWEWRNAIVDRAYAWAHAEEGRKSLYVWAADQKRPD
                     FKQRVKHGVHMALQRRVQFDAFQQFGDAEREACVRLINRFRPHAIVGYTGQLVDIARY
                     VRDHPGLLTWKAKTLVNAAEGLQPGQRELLQRYLVEEVFLSYGCREFMSIGMECDRHV
                     GYHLNTDNLLVEVVGDDGMRVTPGQEGRIVVTDFHNGATPFIRYEIGDIGIMAPTDER
                     CACGKPFPLLKSVDGRLQDVIQTSNGPISALYITYTMRQFDDWIEGYQVVQDTKDRVK
                     VRLLTKSELTPERLGPVEALLRKKLGPSMTIEFERARELTRRRSGKVALVISSIEATQ
                     PATGT"
     gene            3867..>4064
                     /locus_tag="VEC56_07330"
     CDS             3867..>4064
                     /locus_tag="VEC56_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV14001.1"
                     /translation="MPRLLAVVMVFAAAWIAVPAPAQIPDTFTNLKVLPKDIQKAELV
                     GIMRGFSMALNVRCTHCHKGDN"
BASE COUNT          654 a         1356 c         1316 g          738 t
ORIGIN      
        1 gcactcgcgt cgagcgacct cggctccccg ccgtcggccg aaacgagcca cacgccgtgc
       61 gtcgagtcgg cgcgtgaata cgtaaccagc gtcccatccc gcgacacgtc cagcaggttt
      121 tcgcccccgc cgctcgtcaa ctgcgccagc ccggtgccgt cgagcttgat gcgccagatg
      181 tgttgttgcc cgtcgtcgtc gtagcgctcg aagaccacgc ggtcgccgcg cactacaagg
      241 ctcgtcgcgt gaccggggcc caccgcgatg cggcgtggct cgccgccctc gaacggaatc
      301 gacgagatgc cgaggcgacg gtcgtgggcc gcgaggaaca cgacgcggtc ggcggtcagg
      361 tcgaagccga actgcgagtg ctcggggctg gtcgcggagg ttagtcgtct cgtgctccca
      421 tccgccggat cggccacgaa gagattcgtc acctggctca cgcgcaccgc cccgatggcg
      481 ccgctgccac tcgaagtcga gacctgcgag tactggttga aatcgttggt cacgcggcgc
      541 agctcgccgc gcggatacgt gatgacgtac acggcgctgc tcacgccgtc tttcagcacc
      601 gttccggcgg cgacgaggcc gcgtccgtcc ggcatccacg ccaggtcggt gaaaaacgcg
      661 tctttctgga cgaagaagtc ctcgcgcttc ccggactccg cgtcgaacat gatcaggatc
      721 gagcgagccg tcggaacctg ctgaattatg aaaacaccga cgcgctttcc atccggcgac
      781 cacgacggca cccccgagat cacttcgggc cggtagacat tggccagctc gcgctcggcg
      841 cccccatcga tgtcgagcac gaccagcgcg gagcgcgatt cctgcggaac cccgcgcatg
      901 aagcacacgc gcttgccgtc gggcgagaag gtgacgcgcg aatcgacgtc gaaacccttc
      961 tgctcctcgg acccgcccaa cgacggcacg cgcatcagcg ttcgatagtt gggctgctcc
     1021 gggaggcgct tcaagtagaa caggtagttg ccgtcgggcg agaagctcgg cgactcgatg
     1081 gcgacgttcg cgaggggcag gatttcgacg tcgctgccgg tcgcgacttg gcgcacgcgc
     1141 agcgtcgaac gcccggcggc ccccgcgatg tacgcgaggt agcggccgtc gcgagagagc
     1201 gcgctgaagt tcacgtcgcc gcggctggtc tgcgtcacgg cccgcatgtt ctcgaaggga
     1261 ttggcgccct ggtctccgcg gcgcccggcg ctccagattc cgtatccgat gccgagtacg
     1321 ccgatcaaga tcgcgcccgc gaccagcggc gagaaccggc gcgacttgcg cgccacgatc
     1381 gggggcgtct ttcccgtggt cgtcgccgac gtcgccgaag cggacagcgc ctcgtactcg
     1441 tcgacgatct cgcgcaattc gatcgcgagg tccttcatcg actgcaggcg ctggtcgggg
     1501 gccttggcga gacagcggcg aatcagccgc cgcagctcgg ccggggcctg cgggttcaga
     1561 tcctcgaccg gtgtcggctt ctcgttgagg atcttgtgca tcgtctccac gttcgactcg
     1621 gccacgaacg gacgccggcg cgtggccgct tcgtagagca agcaaccgaa ggagaagata
     1681 tccgaccgat ggtcgaccga cttggcctgc acctgctcgg gcgacatgta gtgcacggtg
     1741 ccgagcaccg ctccttcgct cgtgagatcg gcgatttctg tcatcgcggt ggcggacgcc
     1801 gattccagcc gcttctcggt cagcttcgcc agcccgaagt cgagcacctt cgcgaagccg
     1861 tcgtcggaga tcatgatgtt gcccggcttc aagtcgcggt ggacgatgcc ggccgcgtgc
     1921 gccttcgcga tgccctcggc ggcctgcgcg aggtagccga gcaacgtgcg caggtcggtc
     1981 ctttcgtcgt ggatcttcgc acccagcgtc ttcccgctca ccagctccat cgagatgaac
     2041 tggagcggac cgctctccga gccgctgctc gatttgacct tctcgttccc gatctcgtag
     2101 atggtgacga tgttgggatg gttcaaggac gacgccgact tggcctcttg aacgaagcgg
     2161 cgcacgcggt cttcgctgcg cacgaggtcg ggcggcaata ctttgagcgc gacgttacgc
     2221 tcgaggctct ggtcctgcgc gaggtatacc tcgcccatgc cgcccgcacc gagcggtccg
     2281 acgacgcggt attgggcgat gacgtcttcg ggctgtaggg tcttggtcaa cggtttttca
     2341 actccgggtt cgggggagaa tcgggctatt gtagaaggcg tcgcctcagt tgtctacgtg
     2401 cctgtcgcgg gctgagttgc ctcgatcgat gagatcacca aggccacctt gccgctacgc
     2461 cgccgcgtga gctcgcgcgc ccgctcgaac tcgatcgtca tcgacgggcc gagcttcttc
     2521 cggagcaggg cttcgaccgg gcctaaacgc tcgggggtga gctcgctctt ggtgagcagc
     2581 cgcaccttca cgcggtcctt cgtgtcctgg accacctggt agccttcgat ccagtcgtcg
     2641 aactggcgca tcgtgtacgt gatgtacaac gcgctgatcg gtccattcga agtttgaatg
     2701 acatcctgca gtcgtccgtc caccgacttg aggagcggga acggcttgcc gcacgcgcaa
     2761 cgctcgtcgg tcggtgccat gattccaatg tctccgatct cgtatcgaat gaacggtgtc
     2821 gccccgttgt ggaagtcggt gaccacgatg cggccctctt ggccgggcgt cacccgcatc
     2881 ccgtcgtcgc ccaccacttc gaccagcagg ttgtcggtgt tcaggtgata tcccacgtgg
     2941 cgatcgcatt ccatgccgat gctcatgaac tcgcggcacc catacgagag gaacacttcc
     3001 tcgaccaggt accgctgcag caattcgcgc tgccccggct gcagtccttc ggccgcattg
     3061 acgagcgtct tcgccttcca ggtaagaagc cccgggtggt cgcggacgta gcgcgcgatg
     3121 tcgacgagct ggccggtgta accgacgatc gcgtggggcc ggaaccgatt gatgaggcgc
     3181 acgcacgcct cgcgctcggc gtctccgaac tgctggaagg cgtcgaattg gacgcggcgt
     3241 tggagcgcca tgtggacgcc gtgcttgacg cgctgcttga agtccggccg cttctggtcg
     3301 gcggcccaca cgtacaggct cttgcgtccc tcctcggcgt gcgcccacgc gtacgcgcgg
     3361 tcgacgatgg cgttgcgcca ctcccagctc tcgcgcgtga tatggaaccg aatcgggagc
     3421 gaggtcgagc cgctggtctg gtgcgcgaac atcgaatcgg cggggcgctc ctgctgggcg
     3481 aagaactcgc gctggtcgtg gcgcgtccag accggcagct cctcggggcg caagggctcg
     3541 cccggcttgg ggatcgaccc gcggtgcgcc ttcacgcgct ccgcatagta gggaaagcgc
     3601 gcgatcgatc gatagacgtg cccgagaaac tctcgctccc ccaaacccgt aagttcgtcg
     3661 taggacaggc gctggttggc tccgacgcgc cggcggatcg cccggcgccg gtacccccgc
     3721 gcatcgccgt agaggatgct gatcagtacc gcgacgttca tgcgcgaaat ctaccacgcg
     3781 ggccgacgtg cgcaccacgc ttgactcggc gtgctcattg cgcgttcaat ggcggttccc
     3841 cacgccgtgt tcgaaaggag tgttcgatgc ctcgactcct cgcagttgtc atggtattcg
     3901 ccgcggcttg gatcgcggtt ccggccccag cccagatccc cgatacgttc accaacctca
     3961 aggtgctacc gaaggacatc cagaaggcgg agctcgtcgg catcatgcgc gggttctcga
     4021 tggcgctcaa cgtgcgctgc acccactgcc acaagggcga caac
//
LOCUS       DASPGK010000270         6001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_772222, whole genome shotgun sequence.
ACCESSION   DASPGK010000270 DASPGK010000000
VERSION     DASPGK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772222"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VEC56_07335"
     CDS             <1..1153
                     /locus_tag="VEC56_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HXV14002.1"
                     /translation="LKEKIDAGTQPFKGAKFLHTEPHHDDLMLGCLPLIVRHMREHST
                     QHTFATLTSGFTAVTNHFMLAQCDKLLEFLETPMFATLSSQGYFDPDHAGFRNADVWH
                     YLDGVAAGRRTMLDEGEMRRMYRNLVGLFEVKDAEKIADRVEELMDYFETAYPGMKDP
                     DYVQRLKGMCREWEAECLWGYFGWKGDSVRHLRLGFYTGDIFPDEPTRDRDTPPITNL
                     LRETRPDVITLALDPEASGPDTHYKVLQAITESLKIYEKEAGLAEITVLGYRNVWYRF
                     EPSEADIFVPVSLNMFSLQHSAFMNTFASQKDASFPSYEHDGPFSELAQKIQVEQYQT
                     LKTCLGREYFDEHPSPLIRATRGFVFLKRMNLEELYRHSRELKRTTERA"
     gene            complement(1164..2375)
                     /locus_tag="VEC56_07340"
     CDS             complement(1164..2375)
                     /locus_tag="VEC56_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HXV14003.1"
                     /translation="MAISRTFAFLLILFAVACDDESSSSSAFPRCDPDASPTLLASKV
                     DDEDGDGNPLDEDFPDIDDEDDDIADHAWVRDRAGVYHLFFHSESLYHPNQIEHYTTR
                     DFQRLEYVGVALTVEPGAWDADALWAPHVVEHGGVYYMFYTGVSGNNGPDAVQRIGLA
                     TSKNLVTWTRAPVNRCAGTSGDGCIYECDETWTTWGDVGGAYDHQCRDPFVMWDDESG
                     RWLMFVTAKSTNGFGVVTVAGAEELSNWSGEGFIDATRRLASGTDGQTAGGQAENPFV
                     VTTDDTYYLLFSDWQDTEDSVTVAPYRTVVQYATSASLAIDDAGSANWIYRGYTPDPG
                     VNAIEVIEFRRGSGRRWLMSQSISSPRSGFPKPQRRQLWMRCATFEDELIETSNWGLV
                     ANSSGVTAVGR"
     gene            complement(2418..3035)
                     /locus_tag="VEC56_07345"
     CDS             complement(2418..3035)
                     /locus_tag="VEC56_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14004.1"
                     /translation="MIDAPSRQPMESTAHNWKSLRNGVIVSVVMLYLIEPSMKLAVEL
                     APTFGGRFYRLVWDRAAMQASIGGDYLDFAMFAILFSAIVGAFIGRFGAGAAFPRLFG
                     RRGGRKSTDSTLVRWEKRFVVLLMLLVGPIAATTLLVDFAAQQMKLSFDRRITVLAPA
                     IDEESEERLRARFAGLAGRADYQSLNEEMDRLARTTGTDLPEPLL"
     gene            3358..4398
                     /locus_tag="VEC56_07350"
     CDS             3358..4398
                     /locus_tag="VEC56_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14005.1"
                     /translation="MSVDPKSPTSCAVLQPLGIPADTIVVALFDGVDPPRFVFRQLED
                     AAAADTACAARGYYRLAPDESFDDGSVVLQPAEAGDPVIRCVDARGTDPRDLLEEGRL
                     SADLLVLDDRATIEYAASLGRYQDVPLAPDRGYLLLSAARAVVLARGDSLPDLPSPIR
                     SSLALDAVSSRYAHALHLAEWEAIVRGCDAPVGTLAGVRESRAQRVLYNAGDATARDL
                     ADRIVALLNGPEAYSLALAEAIPRLPRAEALFVSVGVLSDDLAERLAAGDDFAYIVSV
                     SLDPARICAEIGRLASSAPWLTRSPDWPREAILPLVKTRPFALAARRPDGFGFDVRPD
                     VRGGLRICGVQP"
     gene            4395..>6001
                     /locus_tag="VEC56_07355"
     CDS             4395..>6001
                     /locus_tag="VEC56_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV14006.1"
                     /translation="MTFRTRLLMGFLAATLIPLVALGLFVRREMTQRLTAQYERQVEA
                     LVVVVENDLERSRARVHAALAEIGEEIIADNRFRRAAMTEGPEERRYLLDYASNTMPV
                     AGLAMLQIQDESGRILSSGHFRNDYDRIDPMLPRLLGQAPERSALVRARTPSGSFLAL
                     ACVDSVRMGNRRFVLVGGVEAESRFLQDLARDDAIRVSLSIPDDALAANADSTSDAIV
                     SQLTIPFAGADSLATATFHVAHDTSELRALRASIDRWFVIAIALAALIALLAATWMAS
                     RLARPLVDLAEKTSRIDLDRLDVDFTNGRKDEIGLLAGTLSRLTTRLRESTVELKDAE
                     RRATFGDLARQVNHDIKNGLTPIRNVFRHLSQQSKEDPAKLPGAFDERSATLDASIAY
                     LENLATSYARLSRRGDRTRCDVNDLVRRIAKDRNDPGKIEVVTKLAGNASVWGDEVSV
                     RRIVENLVANAIESLGGKGRVTITTEYVHRDRSESRIRLTVADTGAGMDAATREKIFD
                     DFYTTKPDGTGLGLSIVRRLVMDLDGT"
BASE COUNT         1057 a         1962 c         1939 g         1043 t
ORIGIN      
        1 cttgaaggaa aagatcgacg ccggcacgca accgttcaaa ggcgcgaagt tcctgcacac
       61 cgagccgcac cacgacgacc tcatgctcgg gtgcttaccg ctgatcgtgc gacacatgcg
      121 cgagcacagc acgcagcaca ccttcgccac cctgaccagc ggctttaccg cggtcaccaa
      181 ccacttcatg ctcgcccagt gcgacaagct gctcgagttc ctcgaaacgc ccatgttcgc
      241 gactctttcg tcgcaggggt acttcgaccc cgaccacgcg ggttttcgca acgccgacgt
      301 gtggcactac ctcgacggcg tggcggcggg cagacgcacc atgctggacg aaggcgagat
      361 gcggcgcatg tatcgcaacc tggtcgggtt gttcgaagtg aaggacgcgg agaaaatcgc
      421 ggaccgtgtc gaagagttga tggactactt cgagacggcg tatccgggca tgaaggatcc
      481 ggactacgtg caacggttga agggtatgtg tcgcgaatgg gaagcggaat gcctgtgggg
      541 atacttcggc tggaagggcg attcggttcg ccacctgcgg ctcggtttct acacgggcga
      601 catctttccc gacgagccga cgcgcgaccg cgacacgccg cccatcacca acttgctgcg
      661 cgagaccaga cccgacgtga tcacgctggc cctcgacccg gaagcgagcg gccccgacac
      721 ccactacaag gtgctgcagg cgatcaccga gtcgctcaag atatacgaga aggaagcggg
      781 gttggcggag atcaccgtgc tcgggtatcg caacgtatgg tatcgcttcg agcccagcga
      841 ggcggacatc ttcgtcccgg tgtcgctcaa catgttctcg ctccagcaca gcgccttcat
      901 gaacacgttc gcgtcgcaga aagacgcgtc gttcccgagc tacgagcacg acggcccctt
      961 ctccgagctg gcgcagaaga tccaggtcga gcagtaccag accctcaaga cctgcctggg
     1021 ccgcgagtac ttcgacgaac accccagccc cttgatccgc gccacgcgcg gcttcgtgtt
     1081 cttgaagcgg atgaacctcg aggaattgta ccgccactcg cgcgagctca agcggacaac
     1141 ggagagggcc tagcggcgcg tcttcagcga ccgacggcgg tcacgcccga gctgttggca
     1201 accaaccccc agttcgacgt ctcgatgagt tcgtcctcga aggtcgcgca ccgcatccag
     1261 agctgacgcc gctgcggctt ggggaagccc gagcgcgggc tcgagatgct ctgcgacatg
     1321 agccaccgcc tcccgctgcc gcgccgaaac tcgatcacct cgatggcgtt gacaccgggg
     1381 tcgggcgtgt aaccgcggta gatccaattc gcgctgccgg cgtcatcgat ggcgagcgac
     1441 gccgatgtcg cgtattgaac gacggtgcgg tacggcgcga ccgtcacgct gtcttcggta
     1501 tcctgccaat ccgagaagag caaatagtag gtatcgtcgg tggtgacgac gaagggattc
     1561 tcggcttgcc cgccggcggt ctggccgtcg gtgccgctcg cgagacggcg tgtggcgtcg
     1621 atgaagccct cgccgctcca attcgaaagc tcttccgcgc ccgccaccgt gaccacgccg
     1681 aagccgttcg tgctcttcgc ggtcacgaac atgagccagc gaccgctctc gtcgtcccac
     1741 atcacgaatg ggtcgcggca ctggtggtcg tacgcgccgc ccacgtcgcc ccacgtcgtc
     1801 caggtctcgt cgcactcgta gatgcagccg tcgccgctcg tccctgcaca ccgattgacg
     1861 ggggcgcgcg tccacgtcac gaggttcttc gacgtggcga ggccgatgcg ttggacggcg
     1921 tcgggtccgt tgttgcccga gaccccggta tagaacatgt aatagacgcc gccgtgctcg
     1981 acgacgtgcg gcgcccacaa cgcgtctgcg tcccacgcgc ccggttcgac cgtcagcgcg
     2041 acgccgacgt actccaggcg ctggaagtcg cgcgtcgtgt agtgctcgat ttgattgggg
     2101 tgatagaggc tctcgctgtg gaagaagagg tggtagacgc ccgcacgatc gcgcacccac
     2161 gcgtggtccg cgatgtcgtc gtcctcgtcg tcgatgtcgg ggaagtcctc gtcgagcgga
     2221 ttaccgtcgc cgtcctcgtc atcgaccttg gaggcgagca gggtgggcga cgcgtcgggg
     2281 tcgcaccggg ggaacgcgct actggacgac gattcgtcgt cgcacgcgac ggcgaacagg
     2341 atcagcagga atgcgaaggt gcggctaatg gccatgcgtc gaccgagggg aggtcgtagc
     2401 catcatacca cgagtggtta cagcagcggc tccggcaagt cggtgccggt cgttcgcgcg
     2461 aggcgatcca tctcctcgtt gagactctga tagtccgcgc ggccagcaag gcccgcgaag
     2521 cgcgcgcgca ggcgctcttc ggactcttcg tcgatcgcgg gtgcgaggac cgtgatgcga
     2581 cggtcgaagg agagcttcat ctgctgcgcg gcgaagtcga ccaggagcgt ggttgcggcg
     2641 atgggtccaa ccaagagcat caacagcacg acgaatcgct tctcccagcg taccagcgtc
     2701 gagtcggtgc tcttacgccc gcccctgcgg ccgaacagac gcgggaacgc ggcgcccgcg
     2761 ccgaagcgac cgatgaacgc acccacgatg gcggaaaaca ggatcgcgaa catcgcgaag
     2821 tccaaatagt cgccaccgat cgaggcctgc atggccgcgc ggtcccaaac cagacgatag
     2881 aagcgaccac cgaacgtcgg ggcgagctca accgctagct tcatggacgg ctcgatcaga
     2941 tagagcatca cgacggagac gatgactccg ttgcgcaggc tcttccagtt atgggcggtg
     3001 gattccatgg gctggcgcga aggtgcatca atcatgcctc tcgagaggga accaagcgcc
     3061 ctcgcagacc gccacgagga actctacgcc cgccaccctc attatagcgg ggcgcagaat
     3121 gcgagggtcc tccgtcgacc ccacggtgtt caaggagacg tcgcggctga tggcgccggg
     3181 gcgatcgtcg cgccaagtcc atgtcgggat tgacacccgg cgccccggag tcgttatttg
     3241 aggccagcag ttcgggactg gggtgcgtct gggtccatgc gagttccttc catagttgcc
     3301 tcgatcggtg cggccgtgct ggcgacggcg ttgggatgcg ggccgaagac tgcaacaatg
     3361 tccgtcgatc ccaagtctcc cacatcctgt gcggtgctac agccgctcgg catcccggcg
     3421 gacacgatcg tcgttgcttt gttcgacggc gtcgatccgc cgcgtttcgt gtttcgtcaa
     3481 ctcgaggacg ctgccgccgc cgacaccgcg tgcgcggccc gaggctatta tcggcttgcg
     3541 ccagacgagt ccttcgacga cggcagcgtc gtactgcagc cagccgaagc cggggaccca
     3601 gtgattcggt gcgtcgacgc gcgcggcacg gatcctcgcg acctgctcga ggaagggcgc
     3661 ctgtcggccg atctcctcgt cctcgacgat cgagccacga tcgaatacgc ggccagtttg
     3721 ggccgctacc aggacgtgcc gctggcaccc gatcgtggct acctcctgct ctccgcagcc
     3781 cgcgccgtcg tactcgcgcg cggcgattct ctcccggatc tccccagccc gattcgttct
     3841 tccttggcgc tcgatgctgt cagctcgcgc tacgcgcacg ctctacactt ggccgagtgg
     3901 gaagccatcg ttcggggctg cgacgcgccg gtggggacgc ttgcaggtgt gcgggaatct
     3961 cgggcgcaac gcgtgctcta caatgccggt gatgcgactg cgcgtgatct cgcggaccga
     4021 atcgtggcac tcttgaatgg ccctgaagcc tattccctcg cgttggcaga agccatcccg
     4081 cgccttccgc gcgccgaggc gctgttcgtt tccgtcgggg tcttgtccga cgatctggcg
     4141 gaacgcctcg ccgccggcga cgattttgcg tacatcgtct cggtctcact cgatccggcc
     4201 cgtatttgcg ccgagatcgg tcgactcgcg tcgtcagcac cgtggctgac aaggagtcct
     4261 gactggccac gggaggcgat actcccattg gtcaagacgc gaccgttcgc gctcgcagcg
     4321 cgacgcccgg atggctttgg cttcgacgtt cgccccgatg ttcgcggagg cctccgaatc
     4381 tgcggggttc agccatgacc ttccgcaccc gccttctcat gggctttctc gccgcgacgt
     4441 tgatcccgct cgtggcactc ggacttttcg tgcgccgcga gatgacgcag cggctcacgg
     4501 cgcagtacga acgacaggtc gaagcgctcg tcgtggtggt cgagaacgat ctcgagcgtt
     4561 cgcgcgcgcg cgtgcacgcc gcactggccg aaatcgggga ggagatcatc gccgacaatc
     4621 gcttccgtcg tgccgcgatg acagaggggc cggaggagcg ccgttatctg ctcgactacg
     4681 cgtccaacac gatgccggtc gcggggctcg cgatgctgca gattcaggac gaatcaggtc
     4741 gcatcttgag ctcggggcat tttcgcaacg actacgaccg catcgatccc atgttgccgc
     4801 gcctgcttgg ccaggcgccg gaacgcagcg cgctggttcg agcgcgcacg ccgagcgggt
     4861 cgttcctcgc actggcgtgc gtcgactcgg tgcgaatggg gaatcggcgc ttcgtgctcg
     4921 tgggtggggt cgaagcggag tcacgctttc tccaggacct cgcacgcgac gacgcaattc
     4981 gcgtctcgct gtcgataccg gacgacgcgt tggcggcgaa cgcggactcc acgagtgacg
     5041 cgatcgttag ccagttgacg atcccgttcg cgggtgcgga ttcgctcgcg accgcgacgt
     5101 tccacgtcgc gcacgacacg agtgagttgc gcgcgctccg cgccagcatc gaccggtggt
     5161 tcgtcatcgc catcgcgctc gcggcactga tagctcttct cgcggcgact tggatggcgt
     5221 cgcggctcgc gcgcccgctc gtcgacctgg ccgagaagac gtcgcgcatc gatctcgacc
     5281 gcctggacgt cgacttcacc aacggtcgca aggatgagat cggcctgctc gcggggacgt
     5341 tgagcaggtt gacgacgcga ctgcgcgaga gcaccgtgga gctcaaggac gccgaacgcc
     5401 gcgccacctt cggcgacctg gcacgccagg tgaatcacga catcaagaac gggctcacac
     5461 cgattcgtaa cgtattccgg cacctctcac agcagtccaa agaagacccc gcgaagctgc
     5521 cgggcgcttt cgacgagcgg agtgccaccc tggatgcgag cattgcctac ctcgagaacc
     5581 tcgcgaccag ctatgcgcgc ctctcgcgcc gcggtgaccg cacgcgctgc gacgtcaacg
     5641 acctggtccg ccgcatcgcg aaagatcgca atgacccggg caagatcgaa gtcgtgacca
     5701 aactggcggg gaacgcgagc gtgtggggcg acgaggtgtc ggtgcgacgc atcgtcgaga
     5761 atctggtcgc caacgcgatc gagagcctcg gcggcaaggg acgcgtgacg atcaccaccg
     5821 agtacgtgca ccgcgaccgc agtgagtcgc gtattcgatt gacggtcgcg gacaccggcg
     5881 ccggtatgga cgcggccacg cgggagaaga tcttcgacga cttctacacc acgaagcccg
     5941 acggcaccgg tctcggtctt tcgatcgtgc ggcgcctggt gatggacttg gacggcacta
     6001 t
//
LOCUS       DASPGK010000271         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_305400, whole genome shotgun sequence.
ACCESSION   DASPGK010000271 DASPGK010000000
VERSION     DASPGK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305400"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VEC56_07360"
     CDS             complement(<1..1019)
                     /locus_tag="VEC56_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV14007.1"
                     /translation="MGHGIPVLMYHSIGRVLADWHWAVLTLPAPTFEDHLRALARGGF
                     TTVGLPELFEYMSGRRTLPERTVVLTLDDGYVDNWTYAVPLLRRYGFCATVLVTPEFV
                     DPRDLLRPTLEDVWAGRGSETDLEVRGFMTWRELAAAAADGALSVESHALTHTWYPTS
                     ADVVDFHHPGDAHYWLDWNAAPAEKPFYLQHLGESRVAWGTPVYRHAKSLAARAYHPD
                     PGEGAYLMTRAAEAGGERLFARSDWRDVLNRALSDFRADHGVRGEPESDRERRARFER
                     ELAGSKQAIEARLGKPVRHLVWPGGGYCDESMDMALTIYDSVTWSGVDRWTLRNRPGE
                     DPRRVT"
     gene            complement(1052..>2876)
                     /locus_tag="VEC56_07365"
     CDS             complement(1052..>2876)
                     /locus_tag="VEC56_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HXV14008.1"
                     /translation="SDRADAAANVAASARTLLHLDSLTMQSGTVDGVGLAQVWRDRAR
                     PDRDWFDDEALAVRVAGHVLLDGPSPRRLVARDLAETYRATGKVPAADYDGAFMIVVV
                     DRARRRVVVANDRVGAFPIYLARHDHAFAFGPEAKSVLAAAGLRARLRPDGVAGLLTF
                     GYCLGATTLFEGVAHLEPASILTLDVASLAHRIERYWNLRFEPSRALRRRSHAENALY
                     ETLLASQRLVLCDQPASYEVMLSGGLDSRGVVAFANVLGKLPSRTFTWGASDAVPKSD
                     AFVARRVAEHFRVPHRFLSYDSGEFVANARDWVYVSELANDNVGWFAEGQPTLAKVYR
                     SGADFAIAGDVVWDSGGFAFSDMEMRRAVLPPALPVPLAACLTPDAGAEYRRLYDHEI
                     AQVLARCDHRDLTDRKEYLYLYARVARYILSLGYYREHAIEVRRPFLSKAALELFASL
                     PRNHRVEKNAYASMLHHRFPELMAIPEQSVWSLPDWERDVRRPGAVRDLWMHYTERER
                     VASGPLAGVLDGDAFAARRDTFFAAVPPPAAKPPLKARFPLRARVLPFVQRHRAIDKL
                     SRLVRVGPGFLPRSDFDLVRCVVLVSMLDESLDRFSSPASR"
BASE COUNT          484 a          985 c         1017 g          390 t
ORIGIN      
        1 gtgacccgcc gcgggtcttc cccgggccgg ttgcgcagcg tccagcgatc caccccgctc
       61 caggtcaccg agtcgtaaat cgtgagagcc atatccatcg attcgtcgca atagccgccg
      121 ccgggccaca ccaggtgtcg cacgggcttt cccagtcgcg cctcgatggc ttgcttcgag
      181 cccgcgagct cgcgttcgaa gcgcgcacgc cgttcgcggt cgctctccgg ctcgcctcgc
      241 acgccgtgat cggctcggaa atcggacagc gcgcgattca gcacgtcgcg ccaatcggaa
      301 cgcgcaaaca gccgctcgcc gccggcttcg gccgcgcgcg tcatcaggta cgccccttcg
      361 ccgggatccg ggtggtacgc gcgcgctgcg agcgacttcg cgtgccgata caccggcgtt
      421 ccccacgcca cgcggctctc tccaaggtgc tgcaagtaga acggcttctc ggcgggcgcg
      481 gcattccaat cgagccagta gtgcgcgtcg cccgggtgat ggaagtcgac cacgtcggcc
      541 gaggtcgggt accaggtgtg cgtcaaggcg tgcgactcga ccgacagcgc gccatccgcg
      601 gccgccgcag ccaactcgcg ccacgtcatg aaaccccgaa cctcgagatc ggtttcgctc
      661 ccgcgccccg cccacacgtc ctccagggtc ggacgcaaaa gatcgcgcgg gtccacgaac
      721 tccggtgtca ccaggacggt ggcgcagaat ccgtagcggc gcagcagcgg tacggcgtag
      781 gtccagttgt cgacataccc gtcgtccaag gtgagcacca cggttcgctc cggcagcgtt
      841 cgccgcccgg acatgtactc gaatagctcg ggaagcccga ccgtcgtgaa tccgccccgc
      901 gctaacgcac gcaggtggtc ttcgaaggtc ggggcgggca aggtcagcac ggcccagtgc
      961 cagtccgcca gcacgcggcc gatgctgtgg tacatcaaga cggggatccc gtggcccatg
     1021 ggcgaagctt cggtcggaac gggccgcgtt gtcaacgcga ggctggggag ctgaaacgat
     1081 ccagcgactc gtcgagcatc gacaccagca cgacgcagcg cacgaggtcg aagtccgagc
     1141 gcgggaggaa cccggggccc acgcgaacca ggcggctcag tttgtcgatc gcacggtggc
     1201 gttggacgaa cgggagcacc cgggcgcgca gcgggaaacg cgccttgagg ggcggcttgg
     1261 cggcgggcgg cggcaccgcg gcgaaaaacg tgtcgcgccg ggccgcgaac gcgtcgccgt
     1321 cgagtacgcc ggccagcggg ccggatgcga cgcgctcgcg ctcggtgtag tgcatccaga
     1381 gatcgcgcac cgcacccggc cggcgcacgt cgcgctccca gtcggggagg ctccatacgg
     1441 actgctcggg aatcgccatc aactccggga aacgatggtg cagcatcgac gcgtaggcat
     1501 ttttctcgac gcggtgattc cgcggcagcg aggcgaataa ctccagcgcc gccttgctca
     1561 agaacggacg gcgcacctcg atcgcgtgct cgcggtagta cccgagcgac aggatgtagc
     1621 gcgcaacgcg cgcgtacagg tacaagtact ccttgcggtc ggtgaggtcg cggtggtcgc
     1681 agcgcgctag cacctgcgcg atctcgtgat cgtagaggcg gcggtattcc gcgcccgcgt
     1741 ccggcgtcag gcacgcggcc agcggtaccg ggagcgcggg cgggagtacc gcccggcgca
     1801 tctccatatc gctgaaggcg aagccgcccg aatcccacac cacgtcgccc gcgatcgcga
     1861 agtccgcgcc cgagcgatag accttcgcga gcgtgggctg cccttccgcg aaccaaccga
     1921 cgttgtcgtt ggcgagctcg gagacgtaca cccagtcgcg tgcattggcg acgaactccc
     1981 cgctgtcata agagaggaac cggtgcggta cgcgaaagtg ctcggccaca cggcgcgcga
     2041 cgaaggcgtc ggacttgggc accgcgtcgg acgcgcccca cgtgaacgtg cggctcggaa
     2101 gcttgcccaa gacgttcgcg aacgccacca cgccgcgcga gtcgaggccg cccgagagca
     2161 tcacctcgta agacgcgggc tggtcgcaca agaccagccg ctgcgaggcg agcagggttt
     2221 cgtacagggc gttctccgcg tgcgagcggc ggcgcagcgc gcgcgagggt tcaaaacgga
     2281 ggttccagta gcgctcgatg cggtgcgcga gcgatgcgac gtcgagagtg aggatgctgg
     2341 ccggctccag gtgcgcaaca ccctcgaaga gagtcgtcgc gccgaggcaa tagccgaagg
     2401 tgagcaggcc ggccaccccg tcggggcgca ggcgggcccg caggccggcg gccgccagta
     2461 ccgacttggc ctcggggccg aacgcgaagg cgtggtcgtg acgcgcgagg taaatcggga
     2521 acgcgcccac gcggtcgttg gcgacgacga cgcggcgccg tgcccggtcg acgaccacga
     2581 tcatgaaggc gccgtcgtag tcggcggcgg gaaccttccc cgtcgcgcgg taggtctcgg
     2641 cgaggtcgcg ggcgacgagg cggcgcggtg aggggccgtc gagcaggacg tggccggcga
     2701 cgcgcaccgc gagggcctcg tcgtcgaacc aatcgcggtc gggacgagcc cggtcgcgcc
     2761 acacttgggc gagcccgacc ccgtcgaccg tgcccgattg catcgtgagc gagtccaggt
     2821 gcagcaacgt gcgcgcggac gcggctacgt tggccgccgc gtccgcccgg tcggac
//
LOCUS       DASPGK010000272        10431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1592921, whole genome shotgun sequence.
ACCESSION   DASPGK010000272 DASPGK010000000
VERSION     DASPGK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592921"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="VEC56_07370"
     CDS             complement(<1..1979)
                     /locus_tag="VEC56_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXV14009.1"
                     /translation="MTDRARIFLTVSMAAVAAFVTVALATTHAGDWPFDRTASLRGVE
                     NHTGPFGSALAFAATALFGRALAWVIPILLAFLSVSIARDAETPVRRLVLKGLLAAVL
                     ANAFFAVSPLSRDVPALRGELGDLIASGLASVFGEVGSAIVLIASVLLIVLGEVHRAR
                     WLAGAASASASRASGLVGSLREMLARRRSVVNDYVKTAVARSAAGSEPDATAAAEDLG
                     PARAASQAERWVEPSARKAAEPDIAISRPRARAEAKPREKKAAKADTGDRTTPLADAS
                     LPPISILVHPQEDGAGFTREELKSWSGVLEEKLTQFGVDGKVTAVHHGPVVTTFEFEP
                     APGVRIKDIVSRSDDLALAMRARSLRMIAPIPGRACVGIEMPNPRGRTVYLAEVLSEV
                     TEKQRTKGIAIGLGVDVVGQPFLMNLCDAPHLLIAGTTGSGKSVCLNTILTSILLHYR
                     PSDVQLLLVDPKMVEMSQYNGIPHLMHPVITDPKDAARVLEFLVGEMSRRNELFRREG
                     VRNIDSYNVKIALEKSEKKGDDLRERLAYIVLVVDELGDLALAKGVDIESLLTRLAQM
                     ARAAGIHMVLATQRPSVDVIVGKTKANFPTRIAFRVATKVDSRTIIDAIGADKLLGKG
                     DMLYMDSHHPQPARLHGAWVSEPEIEQVIAHWKAYR"
     gene            complement(2015..3394)
                     /gene="accC"
                     /locus_tag="VEC56_07375"
     CDS             complement(2015..3394)
                     /gene="accC"
                     /locus_tag="VEC56_07375"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010274050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXV14010.1"
                     /translation="MFQKILVANRGEIALRVMRACNEMGIQTVAVHSEADTDSLHVRF
                     ADEAVCIGPNLPGESYLNFNRIISAAEITSAEAIHPGYGFLSENAEFAEAVVSCGIGW
                     IGPSPSAVEKMGNKSVAKDTMKQAGLPVIPGSDGAVNEEKEALRIAEEIGYPVIIKAA
                     SGGGGRGMRVARDPQDLANAILIARAEAESAFGDASVYLEKYLPTPRHVEVQLLGDSH
                     GNIVHMGERECSIQRRHQKLIEESPCVALTPEVRERLHAAAVQGARAMNYTSAGTMEF
                     LVQGDEFYFMEMNTRIQVEHPVSEMVTGRDLIKEMIAVAAGQPLSFAQKDVRFTGHAI
                     ECRINAEDPTRDFMPCPGKIGFVHLPGGMGVRVDSHIYQGYTITPYYDSMIAKIICLG
                     LDREEAVRRMIRSLEECVVDGVKNTIEFQRAILSHPHFQRGDLSTKFLDGFQWDGKTL
                     SLGEPVVVK"
     gene            complement(3433..4572)
                     /locus_tag="VEC56_07380"
     CDS             complement(3433..4572)
                     /locus_tag="VEC56_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXV14011.1"
                     /translation="MNIKVLLEKMIEMGASDLHIKPGIPPVVRVNGKLQRTNFPAPTP
                     REMEEVARKILTPLQREKFESTREVDFAFGVTGLARFRANFYVQRSSIVMVFRHVPVN
                     IKRVEELMLPPVIGELALRPRGMILVTGTVGSGKSTTLAAMLSTVNQNLNASIITIED
                     PIEFLHRDDKSIVNQREVGSDTASFHDALRHILRQDPNVIMVGEIRDAETMEIALKAA
                     DTGHLVMSTLHTVDAPQTINRIISFFPPHHHQEIRYLLASTLQAVISQRLVRRADGAG
                     RVPAVEVLVTTGTVRECIINAEKTPLLHQAMRDGVTTHQMQTFDQALMKLYQDGIITL
                     DDALRASSKPHELTLRLKGIQATSDQGWKGFENAPPEEPTPAGRK"
     gene            complement(4599..5069)
                     /gene="accB"
                     /locus_tag="VEC56_07385"
     CDS             complement(4599..5069)
                     /gene="accB"
                     /locus_tag="VEC56_07385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXV14012.1"
                     /translation="MRKEEIEELIRLVESSQINELEINEGRRKIRISKGGSASAAAFV
                     PVAPAAPSAPPTRYDEAEEDIESPRGEEKLASNLKQIKSPMVGTFYRSPAPGSEPFTE
                     IGQAVSVGQTVCIVEAMKLMNEIGSDYNGVIRRVLTENGQPVEYGQPLFLVETK"
     gene            complement(5112..5669)
                     /gene="efp"
                     /locus_tag="VEC56_07390"
     CDS             complement(5112..5669)
                     /gene="efp"
                     /locus_tag="VEC56_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXV14013.1"
                     /translation="MADTSDIRNGLIMNMDGEYWAIQEFLHVKPGKGGAFVRTKIKNL
                     LTGQVKDQTFRSGEKINEVRVVRQPFQFLYASGGLYHMMDRATYEQIELPESLVEDAK
                     EFLKENMNLDILMDGDKVLAVELPNFVEMAVTKSEPGFRGDTARGGSKPATLESGLVV
                     QVPLFVNEGDVVRVDTRTGEYVTRV"
     gene            complement(5835..6887)
                     /locus_tag="VEC56_07395"
     CDS             complement(5835..6887)
                     /locus_tag="VEC56_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14014.1"
                     /translation="MKEPIIRTHNREIDDLFERYRRAPGSHVFAPLADAYRKLGLVEE
                     ALDICARGLSANPRYASGYVVQGKCQYDAGRADRAEESFKKVLEIDAQNLVALRYLGI
                     IRAGCGDSDGARGYFERILTLDPGDKDILQRLDAIRESAPVSANTLVEEIEQVLQPEA
                     SKTPTIEIVKERVDVRAEKRVEQPARTAVEARTEDRKVPAEDVIELPEVRDEFEGSPI
                     VLGDDDSVTTEYIATVTLADIFASQGYSEKALKIYHDVLRRQPNNDDVRRKIAAVEKG
                     EPITRPLELETDREHPRPDGIAPVAAPSAIPLPGPNATAPPAGASPVPTTPAGAAIDE
                     GRSYEQFKRWLRTVSD"
     gene            complement(7233..7931)
                     /locus_tag="VEC56_07400"
     CDS             complement(7233..7931)
                     /locus_tag="VEC56_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14015.1"
                     /translation="MNCRRVRHLLLDFVDGLGNEALHAEVDRHVAECVECETFAAETT
                     RSLALLRRTPVEPLDENFNWKVRLAIHREQKASAKRAASAGAWVRAWNFRYVASTGVA
                     FALVLGAGVVTLQRVVPSGPAMPTAARDQYVRPSAPATTEVATHPATPRVTSPRQNLD
                     TPTRFVSEGDAGPSATESAARGAIDPFAQEVAIDSLIDARLLGMTPEKRMRCIQRQIH
                     RLQSQLEKQEAPRP"
     gene            complement(7953..8606)
                     /locus_tag="VEC56_07405"
     CDS             complement(7953..8606)
                     /locus_tag="VEC56_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV14016.1"
                     /translation="MSKLARSLVFTDTNRERAKWKDHTDEELMLRVQGGDTDCFDVLV
                     ERYKVRLFNYLLRLTSNRDEAEEIAQEAFVKAYIHAEKYKTIAKFSTWLYTIATNLVR
                     NRVRSRSRAPQLVSLWTRAWGDGEEERPLEIPDSQRTADEAINDRELSEVISKAIQKI
                     PEKYRESFVLREINELSYEEIAAVTGLKLGTVRSRINRARNYFRQVVAPLVAKGIDF"
     gene            complement(8688..9386)
                     /locus_tag="VEC56_07410"
     CDS             complement(8688..9386)
                     /locus_tag="VEC56_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14017.1"
                     /translation="MIRRARRLAAVTASVLLLATLGCRQRVDANALTGPAACAALDSL
                     LSATGPPRPFAMSGEALLDVEQFRFRGHFEIGVAEGGETVIELGGSTLFGGHREDVAV
                     SLAADTLRVFDRERARFYEGEALDELIRDATRARADWAKVVAQVLALAAPCDDVTTLV
                     RDEDGARAVGPRGTIRVDVDGGRATRTMWPDPVDGSTLGDRLEVRYEWRDGNLARITA
                     SLPARGWRVRLTAK"
     gene            complement(9379..>10431)
                     /locus_tag="VEC56_07415"
     CDS             complement(9379..>10431)
                     /locus_tag="VEC56_07415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14018.1"
                     /translation="RLKDAVSVLETVARAPDAPEIMRLKLAEAYLEADRADDGIRLLE
                     ASRKDPGLSTEGELLLGRLYYQAERYQDALGVFTPLAKRAGNSPELYRILGELHVKTG
                     EAEKARADFERAIAAAPDDFRNYLALFFAYSEEFSKDDVRVKLSKGEATAILAKASAL
                     APRADFDANFMVGMACLSVDSLATARMHLERADALQPDDRGVLFNLASVHEKTREFER
                     AEKILIRLHETHPDDAAICNFYGYLLAEMNKELDLAEDLVRHALSKEPDNGYYIDSLG
                     WVLYKKGDYRNAARELERAIDKLGEDAVILEHLGDAYAALSRYKDALAAYRHSRRLQD
                     NAAVREKIESTERRLD"
BASE COUNT         1658 a         3501 c         3384 g         1888 t
ORIGIN      
        1 cgatacgcct tccaatgcgc gatgacctgc tcgatctccg gctccgacac ccacgcaccg
       61 tgcaggcgcg cgggctgcgg gtggtgcgag tccatgtaca gcatatcgcc cttgccgagc
      121 agcttgtcgg cgccgatggc gtcgatgatg gtgcgcgagt ccaccttggt ggcgacccgg
      181 aacgcgatgc gcgtggggaa gttcgccttg gtcttgccga cgatcacgtc gacggagggc
      241 cgctgcgtcg ccagtaccat gtggatcccg gccgcgcgcg ccatttgggc caggcgcgtc
      301 aatagcgact cgatgtcgac gcccttggcg agcgccaaat cgccgagctc gtccaccacc
      361 aacacgatgt aggccagacg ctcgcgcaga tcgtcgccct tcttctcgga cttctcgagc
      421 gcgatcttga cgttgtagct gtcgatgttg cgcacccctt cgcggcggaa cagctcgttg
      481 cgccggctca tctctcccac caggaactcg agcacgcgcg cggcgtcctt gggatcggtg
      541 atcaccggat gcatgaggtg cggaatgccg ttgtactgcg acatctccac catcttgggg
      601 tcgaccagca agagctgcac gtcggacgga cggtagtgca gcaggatgct ggtcaagatg
      661 gtgttcaagc acacgctctt gcccgacccg gtggtgccgg cgatcaagag atgcggcgcg
      721 tcgcacaggt tcatcaggaa gggctggccc accacgtcga cgcccaagcc gatggcgatg
      781 cccttggtgc gctgcttctc cgtgacttcg ctcaagactt cggccaggta gaccgtgcgc
      841 ccgcgcgggt tgggcatctc gatgccgacg cacgcgcgcc cgggtatggg cgcgatcatg
      901 cgcaggctgc gtgcccgcat cgcgagggcc aagtcgtcgg agcggctgac gatatccttg
      961 attcgcacgc cgggggcggg ctcgaactcg aaggtggtga cgacggggcc gtggtgcacg
     1021 gcggtcacct tgccgtcgac gccgaattgg gtgagcttct cctccagcac cccgctccac
     1081 gacttgagct cctcgcgcgt gaagccggcg ccgtcttcct gcgggtgcac caggatggag
     1141 atgggcggga gcgaggcgtc cgcgagcggt gtggtgcggt cgccggtgtc cgccttcgcg
     1201 gccttctttt cgcgcggctt ggcttccgcg cgcgcgcgcg gacgcgagat ggcgatgtcg
     1261 ggttcggcgg ccttgcgggc ggacggctcg acccagcgct ccgcctggct cgcggcgcgg
     1321 gccggaccca agtcctccgc ggccgccgtc gcgtcgggct ccgagcccgc ggccgatcgc
     1381 gccaccgccg tcttgacgta gtcgtttacg accgagcgcc ggcgcgcgag catctcgcgc
     1441 agcgacccga ccaatcccga cgcccgcgag gccgaggccg aggccgcgcc cgcgagccac
     1501 cgcgcgcggt gcacctcgcc cagcacgatc agcaacacac tggcgatcag cacgatggcc
     1561 gagcccacct cgccgaacac cgacgccagc cccgacgcga tcagatcgcc gagttcgccc
     1621 cgcagcgcgg gcacgtctcg cgacagcggc gagaccgcga agaacgcgtt ggcgagcacg
     1681 gcggccagaa ggcccttgag taccaagcgc cggaccggcg tctccgcgtc gcgcgcgatc
     1741 gacaccgaca ggaacgccag caggatcggg atgacccacg cgagcgcgcg tccaaacagt
     1801 gcagtggccg cgaacgcgag cgcggacccg aacgggcccg tgtggttctc gacaccgcgc
     1861 agcgacgccg tgcgatcgaa cggccagtcg cccgcgtgcg tggtggccag cgcgacggtc
     1921 acgaacgcgg ccaccgctgc catcgacacg gtgagaaaga ttcgggcccg gtccgtcacg
     1981 gctgctcctc gcgccaccct cccggcggcc gacgtcactt cacgaccacc ggctcgccca
     2041 gcgacagtgt cttgccgtcc cactggaagc catcgaggaa cttggtgctc aagtcgccgc
     2101 gctggaagtg ggggtgggag agaatcgcgc gctggaactc gatcgtattc ttcacgccgt
     2161 caacgacaca ttcctccagg gaacggatca tgcggcggac tgcctcctcg cggtcgagcc
     2221 ccaggcagat gattttcgcg atcatcgagt cgtagtaggg cgtgatggtg tagccctggt
     2281 agatgtgcga atcgacgcgc acgcccatgc cgccgggaag gtgcacgaat ccgatcttgc
     2341 cggggcacgg catgaagtcg cgcgtcgggt cctccgcgtt gatgcggcac tcgatggcgt
     2401 ggcccgtgaa gcgcacgtcc ttctgcgcga acgagagcgg ttgccccgcc gccaccgcga
     2461 tcatctcctt gatgaggtcg cggcccgtta ccatctccga caccgggtgc tcgacctgga
     2521 tgcgcgtgtt catctccatg aaatagaact cgtcgccctg gaccaggaac tccatcgtcc
     2581 ccgccgaggt gtagttcatc gcgcgcgcgc cttgcaccgc ggcggcgtgc agacgctcgc
     2641 gcacctcggg cgtcaaggcc acgcacgggg attcttcgat cagcttctgg tggcgccgct
     2701 ggatcgagca ttcgcgttcg cccatgtgga cgatgttgcc gtgcgaatcg ccgagcagtt
     2761 gcacttcgac gtgacgcggt gtaggcaggt acttctcgag gtacaccgag gcgtcgccga
     2821 acgcgctctc ggcttcggcg cgcgcgatca gaatcgcgtt ggccaggtcc tgggggtcgc
     2881 gcgcgacgcg catgccgcgc cccccgccgc ccgaggcggc cttgatgatg accgggtagc
     2941 cgatctcctc ggcgatgcgc agcgcctctt tctcctcgtt gaccgcgccg tccgagccgg
     3001 ggatcacggg caggccggcc tgcttcatgg tgtccttggc caccgacttg ttgcccatct
     3061 tctcgacggc actcggcgac ggcccgatcc acccgatgcc gcacgagacc accgcctccg
     3121 cgaactcggc gttctcggac aggaacccgt agccgggatg gatggcctcc gcgctcgtga
     3181 tttcggccgc ggagatgatg cgattgaaat tgaggtagct ctcgccgggg aggttgggcc
     3241 cgatgcagac ggcctcgtcg gcgaagcgaa cgtgcagaga gtcggtatcg gcttcgctgt
     3301 ggacggcgac ggtctggatg cccatttcgt tgcacgcgcg catcacgcgc aacgcgatct
     3361 cgccccggtt ggcgacgagg atcttctgga acatgggggc tacgctaaca cagccccgaa
     3421 ggtcttgtta acctactttc gccccgccgg ggtgggctcc tccggggggg cgttttcgaa
     3481 gcccttccag ccctggtcgc tggtggcctg gatgcccttc aagcgcagcg tcaactcgtg
     3541 cggcttcgag ctcgcgcgca gcgcgtcgtc gagcgtgatg ataccgtcct ggtagagctt
     3601 catgagcgcc tggtcgaagg tctgcatctg gtgcgtggtc acaccgtcgc gcatcgcctg
     3661 gtgcaacagc ggcgtcttct ccgcgttgat gatgcactcg cggacggtac cggtcgtgac
     3721 cagcacttcg acggcgggca cgcgccccgc gccgtcggcg cgccgcacca gtcgctgcga
     3781 gatgaccgcc tgcagcgtcg acgccaggag gtagcggatc tcctggtggt ggtgcggcgg
     3841 gaagaacgag atgatgcggt tgatggtctg cggcgcgtcc accgtatgca gcgtgctcat
     3901 gacgaggtgg ccggtgtcgg ccgccttgag cgcgatctcc atggtctcgg cgtcgcgaat
     3961 ctcacccacc atgatgacgt tggggtcctg gcgcaggatg tggcgcagcg cgtcgtggaa
     4021 ggacgcggta tcgctgccca cctcgcgctg gttgacgatg cttttgtcgt cgcgatggag
     4081 aaactcgatg gggtcctcga tggtgatgat ggacgcattt aggttctgat tgacggtgga
     4141 tagcatcgcg gccagcgtgg tcgacttgcc gctgcccacc gtgcccgtca ccaggatcat
     4201 gccgcgcggg cgcagcgcca actcgccgat caccggcggc agcatgagtt cttcgacgcg
     4261 cttgatattg acggggacat gacggaaaac catcacgatc gaactgcgct ggacgtagaa
     4321 gttggcgcgg aagcgtgcaa gaccggtgac cccgaatgca aaatcgacct cgcgggtgct
     4381 ctcgaatttc tcgcgctgga ggggggtcag gattttccgg gccacctctt ccatctcgcg
     4441 cggagtgggg gccgggaagt tggtccgttg cagtttgcca ttaacgcgga cgaccggcgg
     4501 aatgcccggc ttgatgtgga ggtccgaggc ccccatctcg atcatcttct cgaggagcac
     4561 cttgatgttc atttgccgag acctttccgc tatttaactt atttcgtttc aacgagaaac
     4621 agcggctgcc cgtactccac cggttggcca ttctcggtca acacccgccg aatgaccccg
     4681 ttgtagtccg agccgatctc gttcatgagc ttcatcgcct ccacgatgca caccgtctgc
     4741 cccaccgaca ctgcttgccc aatttccgtg aatggctcgg agccgggagc cggcgacctg
     4801 tagaacgtgc cgaccatcgg tgacttgatc tgtttcaagt ttgatgccag cttctcctcg
     4861 ccacgcgggc tttcaatatc ctcctccgcc tcgtcgtacc gcgtcggggg cgccgaggga
     4921 gcggcgggtg ccaccggcac gaacgccgcc gcggacgccg agccgccctt cgagatacga
     4981 atcttcctgc gcccttcgtt gatctcgagc tcgttgatct ggctcgattc gaccaagcgg
     5041 atcagttctt cgatttcttc cttacgcatc ttcacctccg gtcgtcgaaa cgggtcgtgc
     5101 gaacgccgcg gtcatacccg cgtcacgtac tcgccggtcc gagtgtcgac gcgaaccacg
     5161 tcgccctcgt tcacgaacaa cggcacctgg accaccaacc cggactccag cgtggccggc
     5221 ttggaaccgc cgcgcgcggt atcgccgcgg aaaccgggct ccgacttcgt caccgccatc
     5281 tccacgaagt tgggcaactc cacggcgagc accttgtcgc cgtccatgag aatgtcgaga
     5341 ttcatgttct ccttgagaaa ctccttggcg tcctcgacca gcgactcggg caactcgatc
     5401 tgctcgtagg tcgcgcggtc catcatgtgg tagagcccgc ccgacgcata caagaattga
     5461 aacggctgcc ggaccacgcg gacctcgttg atcttctcgc ccgagcggaa ggtctgatcc
     5521 ttgacctggc cggtcagtag gttcttgatc ttggtgcgga cgaacgcccc gcccttgccg
     5581 ggcttgacgt ggaggaactc ctggatcgcc cagtactcgc cgtccatgtt catgatcagc
     5641 ccgttgcgga tatcgctggt gtcggccatt cctctatctc cttgatttcg tgattattag
     5701 ctgatttggt cagtcccgat tttcgccgcc gattctaatt cgggacagcc ggggtttcca
     5761 gcacgaagtt tggaccgcag cgcgccggtc gaggaggctt tggcgggggg aaatcggcgg
     5821 acggacgggc cgaatcagtc ggagacggtg cggagccagc gcttgaactg ctcgtagctg
     5881 cgcccctcat cgatagcagc ccccgcgggg gtcgtcggca cgggcgaggc gccggccggc
     5941 ggggcagtcg cattcggtcc cggcagcggg atcgcgctcg gcgccgcgac gggagcgatc
     6001 ccgtcggggc gggggtgctc gcgatccgtc tcgagctcga gcgggcgcgt tatcggctcg
     6061 cccttctcga cggccgctat cttccggcgc acgtcgtcgt tgttcggctg ccgacgcaag
     6121 acgtcgtggt agatcttgag cgctttctcc gagtacccct gggacgcgaa gatgtccgcg
     6181 agcgtcaccg tcgcgatgta ctcggtggtc accgaatcgt cgtcgcccag cacgatcgga
     6241 gagccctcga actcgtcgcg cacctcgggc agctcgatca cgtcctccgc gggaaccttg
     6301 cggtcttcgg ttcgggcttc gacggccgtg cgcgccggtt gctcgacgcg cttctcggcg
     6361 cgcacgtcca cgcgctcctt cacaatctcg atagtgggcg tcttggacgc ttcgggctgg
     6421 agcacctgct cgatctcttc gacgagcgtg ttcgcggaca ccggcgcgga ctcgcggatg
     6481 gcatcgagtc gctggaggat gtccttgtcg cccgggtcga gcgtcaagat ccgctcgaag
     6541 taaccgcgcg cgccatcgga gtccccacag cccgcgcgaa tgatccccag gtaacgcagc
     6601 gcgaccaggt tctgggcgtc gatctcgagc accttcttga acgactcttc cgcgcggtcg
     6661 gcgcgaccgg cgtcgtactg gcacttgccc tggacgacgt aaccactcgc atagcgcggg
     6721 ttggccgaca gcccgcgcgc acagatgtcg agcgcttctt cgaccaggcc cagctttcgg
     6781 taggcgtccg ccaggggggc gaagacgtgg cttccgggcg cacggcggta acgctcgaag
     6841 agatcgtcga tctcgcggtt gtgagtcctg atgatcggtt ctttcatccc aaataacccg
     6901 cggattgcgg ccaaaacagg ccgcccgcgt cgatggatgg tcgacttcgg tccggcgaag
     6961 ccgcggccac ggtgggcagg gcccgcgtaa ccacttgtga taccgcgcga tcgcgcgacc
     7021 gctcgaaacg ccggacactc ctgtagcagg cgcaacttgc accaaacgtg ccagtttacg
     7081 acaccacggg acgcgagccg cgaccacggg acacgaccgg ccctaggccg gcgagcgaga
     7141 gcataccgct caggctgcgc actcggcaaa aagaatcggc ccgcccctcg aaagaaggac
     7201 gggccgaagc ggccggtctc gcggtgtcga aactacgggc gcggcgcttc ctgtttttcc
     7261 aactgtgact ggaggcggtg gatctggcgc tggatgcagc gcatacgctt ctccggcgtc
     7321 atgccgagca agcgggcgtc gatcaacgaa tcgatcgcca cttcctgggc gaacggatcg
     7381 atggcgccgc gcgcggccga ctcggtcgcg ctcggaccgg catcgccctc gctgacgaaa
     7441 cgagtggggg tgtccaggtt ctgtctcggg ctggtcacgc gcggcgtcgc cgggtgagtc
     7501 gcgacttcgg tcgtcgcggg cgccgagggg cgcacatatt gatcgcgcgc ggcggtcggc
     7561 attgcgggtc cgctcgggac gacgcgctgc aacgtcacca cacccgctcc cagcacgagc
     7621 gcgaacgcga ctccggtgct ggcgacgtag cggaaattcc acgcgcgcac ccacgcgccc
     7681 gcggaggccg cgcgcttggc actggccttc tgctcgcgat ggatggccag tctcaccttc
     7741 cagttgaaat tctcgtcgag aggctcgacc ggggtacgcc gcaggagcgc caagctccgc
     7801 gtcgtctccg ccgcgaacgt ctcgcattcg acgcactcgg cgacgtggcg atccacttcg
     7861 gcgtggagcg cctcgttacc caggccatcg acgaagtcga gcagcagatg tcgaacacga
     7921 cggcagttca tcggaccatt cctcctcgag acctagaagt cgattccctt cgcgaccagc
     7981 ggcgctacca cctgccggaa atagttccgc gcgcgattga tgcgcgaccg aaccgttccc
     8041 aacttcaaac ccgtgacggc cgcgatctcc tcgtacgaaa gctcgttgat ctcacgcagc
     8101 acgaagcttt cccggtactt ctccggaatc ttctggatcg ccttgctgat gacctcgctc
     8161 aactcgcgat cgttgatggc ctcgtcggcg gtgcgctgcg agtccggaat ctccagcggc
     8221 cgctcttctt caccgtcgcc ccaggcgcgc gtccacagcg agaccaattg cggcgcgcgc
     8281 gaccgcgatc gtacccggtt ccgcaccagg ttggtcgcga tggtatagag ccaggtcgag
     8341 aacttcgcga tcgtcttgta cttctcggcg tggatgtacg ccttgacgaa ggcttcctgc
     8401 gcgatctctt ccgcctcgtc gcggttgctc gtcagccgca acaggtagtt gaacaagcgc
     8461 accttgtagc gctcgaccag cacgtcgaaa cagtcggtat ccccaccctg gacgcggagc
     8521 atcaactcct cgtcggtgtg atccttccac ttcgcgcgtt cgcggttcgt atccgtgaac
     8581 accaggctcc tcgcgagttt ggacatccag cctcgcgccc ttataccttt gggggcgccg
     8641 ccggttcccg ctccatcttg tcgtgattac aacacgttat aaaacaatca tttagctgtc
     8701 agtcgaaccc gccatccgcg cgccggcagg ctggcagtga tgcgcgccag gttcccgtcg
     8761 cgccattcgt agcgcacctc gagccggtcg cccagggtgc tgccgtccac cgggtcgggc
     8821 cacatcgttc gggtcgcacg cccgccgtcg acatccacgc gaatggttcc gcgaggaccc
     8881 accgcgcggg ccccgtcttc gtcgcgcacg agcgtcgtga cgtcgtcgca gggggccgcc
     8941 agcgccagca cctgcgccac caccttcgcc cagtcggcgc gcgcgcgggt ggcgtcacga
     9001 atcagctcat cgagcgcttc cccctcgtag aatcgggccc gctcgcggtc gaatacacgc
     9061 agggtgtcgg cggcgagcga caccgcgacg tcttcgcggt ggcccccgaa gagcgtgctg
     9121 ccgcccagct cgatcacggt ttcaccgccc tcggcaacgc cgatctcgaa atgaccacgg
     9181 aaccggaact gttcgacgtc caggagcgcc tcgccggaca tggcgaacgg gcgcggcggt
     9241 cccgtcgcgg acaagagcga gtcgagcgcc gcgcacgccg cgggtcccgt gagcgcgttc
     9301 gcgtcgacgc gctggcggca tcccaaggtc gcgagcaaca gcaccgacgc ggtcaccgcg
     9361 gcgagccggc gggcgcgcct aatcaaggcg ccgttccgtc gattcgatct tctctcgcac
     9421 cgcggcgttg tcctgaagac ggcgcgagtg tcgataggcc gcgagcgcgt ccttgtagcg
     9481 agaaagtgcg gcgtaggcgt cgcccagatg ctcgaggatc acggcgtctt cgcccagctt
     9541 gtcaatggcg cgctcgagct cgcgggccgc gttacggtag tcgcccttct tatagagcac
     9601 ccatcccagg ctgtcgatgt agtagccgtt gtcgggctcc ttggacagcg cgtgtcgcac
     9661 caggtcctcg gccaaatcga gctccttgtt catctcggcc aggagataac cgtagaagtt
     9721 gcatatcgcc gcgtcgtcgg ggtgggtctc gtgcaggcga atcaggatct tctccgcgcg
     9781 ctcgaactcg cgcgtcttct cgtgcaccga cgccaggttg aagagtacgc cgcggtcgtc
     9841 gggctggagc gcgtcggcgc gctcgaggtg catgcgcgcg gtcgccagcg aatccacgct
     9901 caggcacgcc attccgacca tgaaattcgc gtcgaagtcg gcgcgcggcg cgagcgccga
     9961 ggccttggcc aggatcgcgg tggcttcgcc cttggacagc ttcaccctca catcgtcctt
    10021 cgagaattcc tcggagtagg cgaagaagag ggccaggtag ttgcgaaaat cgtcgggcgc
    10081 ggccgcgatg gcgcgttcga agtcggcccg cgctttctcg gcctcgcccg tcttcacgtg
    10141 cagctcgccc agaatgcgat agagctccgg actgttgccc gcgcgcttcg cgagcggcgt
    10201 gaacacgccc agcgcgtctt gataacgctc ggcctgatag tagaggcggc cgagcagaag
    10261 ctcgccctcg gtggacaacc cgggatcctt ccgcgatgct tcgagcaggc gaattccgtc
    10321 gtcggcgcgg tcggcctcga ggtacgcctc ggcgagcttc aggcgcatga tctcgggcgc
    10381 gtcgggcgcg cgcgccacgg tttcgagcac cgagaccgcg tccttgaggc g
//
LOCUS       DASPGK010000273         5650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_70558, whole genome shotgun sequence.
ACCESSION   DASPGK010000273 DASPGK010000000
VERSION     DASPGK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70558"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1149)
                     /locus_tag="VEC56_07420"
     CDS             complement(130..1149)
                     /locus_tag="VEC56_07420"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HXV14019.1"
                     /translation="MAERPHEKRLLNCSATDPNKILPMKYNWARKHYKDGVANNWTPE
                     EVNMQKDVEMWRSPDALSSHERRLVLWNMGFFSTAESLTANNIVLTVYRHVTNPECRQ
                     YLLRQAFEEAIHTDTFIYCCDTLGLDPDEVYSMYMSIPSIKAKDDFVIDITKSILDPN
                     FNTEGRENIQKFVHDLVGFYVIMEGIFFYAGFVMMLSFLRQNKMVGVGEQFQFILRDE
                     SVHLAFGCDLINNIVLENPEIWTEDFKATLVANIKRAVELEHAYARDCLPKGILGLNA
                     DAIHQYVKYVADRRLERINLPKLYNVANPFPWMSEVIDLKKEKNFFETRVTEYKTGGV
                     LSWEE"
     gene            complement(1170..3932)
                     /locus_tag="VEC56_07425"
     CDS             complement(1170..3932)
                     /locus_tag="VEC56_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HXV14020.1"
                     /translation="MEISKIKKRNGSIVDFDRTRIEHAIEKACNATGVTVASEFYSSV
                     TDDVTGLIEQKFDERIPGVEDIQDVVEMMLAERGLFEVAKAYILYRKEREAVRRHKQD
                     ELLEKIDRREIKVVKRSGETVEFNIGEIEKAITNCCIGLEGSIDVIGILRDTKLGLYD
                     GIKTSEINQAVIMSIRARIERDPVYSTLAARFLFNDLYKAVLGMDEFADTFAEAHRAG
                     FAHKIKAGVDAGRLDPAMLEFDLARLGAELRPERDTLFTYLGAQVLYDRYFLKSLKQE
                     ILETPQYFWMRIAMGLAMNEPKDREDWALKFYDVISSLHYVPSTPTLFHSGTNHPQMS
                     SCYLTTVKDDLSHILKCVGDNAQLSKWSGGLGNDWTNIRGTGAQIKSTNVGSQGVVPF
                     LKIVDATTAAINRSGKRRGATCVYLECWHYDFEDFLELRKNTGDERRRTHDTNTASWI
                     PDLFMKRVLADGTWTLFSPDETPDLHELYGRAFELRYEEYERMADAGQIRLFRKLRAI
                     DLWRRMITMLYETGHPWITFKDPCNLRSPQDHVGVVHSSNLCTEITLNTSEDETAVCN
                     LGSINLARHVDDGKLAYDRLKYTIKMAVRMLDNVVDLNFYPTEEARRANLRHRPIGLG
                     VMGLQDALYLVNLPFGDDGAVEFSDNLQEFVSYHAILASSELAEEKGAYETYRGSKWD
                     RGLFPVDTLDLLESERGMSTGVDRRSWMDWTPVRERVKQHGIRNSNMMAIAPTATIAN
                     IAGCVPSIEPIYKNMYVKSNFSGEFTIINRFLVSDLKELGLWGDDMMEKIKYYDGSVQ
                     AIQEIPAEVRAKYQEVFEIDPVWIVKHAAHRGKWIDQSQSVNIFTTSTSGKQISDTYM
                     TAWKMGLKTTYYLRTLGASGIEKSTLDSKAKKFAGAAHVVPQQVPATQVAKACSIEDP
                     TCEACQ"
     gene            4285..4971
                     /locus_tag="VEC56_07430"
     CDS             4285..4971
                     /locus_tag="VEC56_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXV14021.1"
                     /translation="MTTVTDNVARVRERVDSALRRAGRAAGEVTIVAVTKTFGPEMVR
                     AAIEAGIADIGENRVQELLEKQRALDPSPCRWHLVGPLQRNKAGKVVGRVDLFHAIDG
                     VRIAETVDRLARERALRARVLLEVNMSGEASKHGVTVAEAPAAAEALSRLDGLEWLGL
                     MTMGPLSGGADETRACFRSLARLGAQLRGVTGRPLPVLSMGMSDDFEVAIEEGATMIR
                     VGRGITGERV"
     gene            5188..>5650
                     /locus_tag="VEC56_07435"
     CDS             5188..>5650
                     /locus_tag="VEC56_07435"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXV14022.1"
                     /translation="MRITPLDVRKQEFRKAMRGLDGDEVYAFLSTVADEYEAVLNDNK
                     ALRERLLELDDKVQEYRTIERTLRDTLLTAERVTVEAKDNARREATLIVKEAQLEAQK
                     ALRDITTEGARLRQEVQRLRSQRDAYIAKMKVVAESHVKFIESAARDFHVED"
BASE COUNT          965 a         1703 c         1865 g         1117 t
ORIGIN      
        1 acctcgaaag atggcccgga gtatatcgcg cgacccgcgc gccgccaaag cagaatgtgg
       61 gcacaaaaag caagacgccc cgccgggcga aggcggggcg tcgaccgaag gcggctgctg
      121 ccgacgggcc tactcttccc agctcaagac gccgcccgtc ttgtactccg tgacgcgcgt
      181 ctcgaagaag ttcttttcct tcttgaggtc gatgacctcg ctcatccacg ggaacgggtt
      241 ggcgacgttg tagagcttgg ggaggttgat ccgttccagc cggcgatccg cgacgtactt
      301 gacgtactgg tggatggcgt cggcattgag gcccaagatg cccttgggca ggcagtcgcg
      361 cgcgtacgcg tgctcgagct cgaccgcgcg cttgatattg gcgaccagcg tggccttgaa
      421 gtcttcggtc cagatctccg ggttttcgag cacgatgttg ttgatgaggt cgcagccgaa
      481 ggcgagatgg accgattcgt ctctcagaat gaattggaac tgctcgccca cgcccaccat
      541 cttgttctgt cgcaggaacg acagcatcat cacgaagccc gcgtagaaga agatgccttc
      601 catgatgacg tagaagccca ccaagtcgtg gacgaacttc tggatgttct cgcggccctc
      661 ggtgttgaaa ttggggtcca agatcgactt ggtgatgtcg atcacgaagt cgtccttggc
      721 cttgatgctc gggatgctca tgtacatgct gtacacctcg tcggggtcga gcccgagcgt
      781 gtcgcagcag taaatgaacg tgtcggtgtg gatcgcctcc tcgaacgcct ggcgcaggag
      841 gtactgccgg cactcggggt tggtgacgtg gcggtatacc gtgagcacga tgttgttcgc
      901 agtcaggctc tccgcggtcg agaagaagcc catgttccac agcaccaggc gccgttcgtg
      961 cgacgagagc gcgtcggggc tgcgccacat ctcgacgtcc ttctgcatgt tcacttcttc
     1021 cggggtccag ttgttggcca ccccgtcctt gtagtgcttg cgcgcccagt tgtacttcat
     1081 gggcaggatc ttgttgggat ccgtggcgga gcaattgagg agtcgtttct cgtgcggtcg
     1141 ttcagccatg gtgctttcct ccgccgtgcc tactggcagg cttcgcaagt cgggtcttcg
     1201 atcgagcacg ccttcgcgac ctgggtcgcg ggaacttgtt gcggcaccac gtgcgccgcg
     1261 cccgcgaact tcttggcctt ggagtcgagc gtcgactttt cgatccccga cgcccccagc
     1321 gtgcgcaaat agtacgtcgt cttcaggccc attttccacg ccgtcatgta ggtgtcggag
     1381 atctgcttgc ccgacgtgct ggtggtgaag atgttgaccg attggctctg gtcgatccac
     1441 ttgccgcggt gtgcggcgtg cttgacgatc cacaccgggt cgatctcgaa cacttcctgg
     1501 tacttcgcgc gcacctcggc cggtatctcc tggatcgcct gcacgctgcc gtcgtagtac
     1561 tttatcttct ccatcatgtc gtcgccccac aagccgagct ccttcagatc gctcaccagg
     1621 aatcgattga tgatggtgaa ctcgccgctg aagttcgact tcacgtacat gttcttgtaa
     1681 atgggctcga tcgacggcac gcaacccgcg atgttggcga tggtcgcggt gggcgcgatc
     1741 gccatcatgt tcgagttgcg aatcccgtgc tgctttacgc gctcgcgcac gggcgtccag
     1801 tccatccacg agcgacggtc gactccggtc gacatgccgc gctcgctctc gagcaggtcc
     1861 agcgtgtcga cggggaagag gccgcgatcc cacttggagc cccggtaggt ctcgtacgcg
     1921 cccttctcct cggccagctc cgacgaggcc aggatcgcgt ggtaggagac gaactcctgc
     1981 agattgtccg agaactccac cgcgccgtcg tcgccgaagg ggaggttcac caggtagagc
     2041 gcgtcctgga gccccatgac cccgagcccg atggggcggt ggcgcaggtt ggcgcgccgc
     2101 gcctcctcgg tgggatagaa gttcaagtcg acgacgttgt cgagcatgcg gaccgccatc
     2161 ttgatggtgt acttcaggcg gtcgtaggcg agcttgccgt cgtcgacgtg gcgcgccaga
     2221 ttgatcgacc ccaggttgca caccgcggtc tcgtcttcgg aggtgttgag cgtgatctcg
     2281 gtgcacaggt tcgagctgtg cacgacgccg acgtgatcct gcggcgagcg caggttgcac
     2341 ggatccttga acgtgatcca gggatgcccg gtctcgtaga gcatcgtgat catgcggcgc
     2401 cacaggtcga tcgcgcgcag cttccggaac aggcgaatct ggccggcatc cgccatgcgc
     2461 tcgtattcct cgtagcgcag ctcgaaggcg cggccgtaca gctcgtgcag gtccggggtc
     2521 tcgtcgggcg agaacagcgt ccaggtgccg tcggccagca cgcgcttcat gaacagatcg
     2581 ggaatccagc tcgcggtgtt ggtgtcgtgc gtgcggcggc gctcgtcgcc ggtgttcttg
     2641 cgcagctcga ggaagtcctc gaagtcgtag tgccagcact cgaggtacac gcaagtggcg
     2701 ccgcggcgct tgccgctgcg attgatcgcg gcggtggtgg cgtcgacgat cttcaagaag
     2761 ggaaccacgc cctggctacc cacgttggtg ctcttgattt gggcgccggt gccgcggatg
     2821 ttggtccaat cgttaccgag gccgcccgac cacttggaga gctgggcgtt gtcgcccacg
     2881 cacttcaaga tatgcgagag gtcgtccttg acggtggtta gatagcacga gctcatctgc
     2941 gggtggttgg ttcccgagtg gaagagcgtg ggcgtcgacg gcacgtagtg cagcgacgag
     3001 atgacgtcgt agaacttgag cgcccagtct tcgcgatcct tgggttcgtt catggcgagc
     3061 cccatcgcga tgcgcatcca gaagtactgc ggcgtctcga ggatctcctg cttgagactc
     3121 ttcaagaagt agcggtcgta gagcacctgc gcgccgaggt acgtaaagag cgtgtcgcgc
     3181 tcggggcgca gctcggcgcc caggcgcgcg aggtcgaatt cgagcatggc gggatccaag
     3241 cggcccgcgt ccacgccggc cttgatcttg tgcgcgaaac ccgcgcggtg cgcttcggcg
     3301 aatgtgtctg cgaactcgtc catgccgagg acggccttat ataggtcatt gaacaggaag
     3361 cgagccgcga gcgtcgagta gacggggtcg cgctcgatgc gcgcgcggat ggacatgatg
     3421 acggcctgat tgatctccga ggtcttgatg ccgtcgtaga gccccagctt ggtatcgcgc
     3481 aggatgccga tcacgtcgat cgagccttcc aggccgatgc agcaattggt gatcgccttt
     3541 tcgatctcgc cgatgttgaa ctcgacggtt tcgccgctgc gcttgacgac cttgatctcg
     3601 cggcgatcga tcttctccag gagctcgtcc tgcttgtgac ggcggacagc ctcgcgctcc
     3661 ttgcggtaca ggatgtaggc cttggccacc tcgaacagcc cgcgctcggc gagcatcatc
     3721 tcgacgacgt cctggatgtc ctccacgccg gggatgcgct cgtcgaactt ctgctcgatg
     3781 agcccggtga cgtcgtcggt caccgaggag tagaactcgg aagcgacggt gacgccggtc
     3841 gcgttgcacg ccttttcgat ggcgtgctcg atgcgggtgc ggtcgaagtc gacgatcgag
     3901 ccgttgcgct tcttgatctt gctgatctcc ataggactcc tagcggctgt ggggccgaaa
     3961 accggctaac ctaataccta ttaacgggtt atagaatcaa tcgggttcgg ggtccgctcc
     4021 ccgtcggtgg ccggcccacg taactcgttg cggcgcctga cgcaacaggt tgggtgggtc
     4081 ggctcgagga ccccctaccg attgaggttc ggccatagta cggacgaccc catgggtcgt
     4141 caagagtttc tccgggttgt gaaatgttta tcttcgccct gcttatcttg ttgacacacc
     4201 ataagatatg cagattccgc ccacccggca atgtggaatc ttggtggatg gcaggtggat
     4261 aacattcggc tagaatgcac ccccatgacg accgtcaccg ataacgtcgc gcgcgtgcgc
     4321 gaacgggtcg attcggccct gcggcgcgct gggcgcgccg caggcgaggt cacgatcgtc
     4381 gccgttacga aaacgttcgg tcccgagatg gtgcgcgccg caatcgaagc cggcatcgcc
     4441 gacatcggcg agaaccgcgt gcaggagctg ctggagaagc agcgcgcgct cgatccatcg
     4501 ccgtgccgct ggcatctggt ggggccgctg cagcgcaaca aggccggcaa ggtggtgggg
     4561 cgcgtcgacc tgttccacgc gatcgacggg gttcgcatcg ccgaaaccgt cgaccggctg
     4621 gcgcgcgaac gcgcgctgcg cgcgcgtgtg ctcctcgaag tgaacatgtc gggagaggcg
     4681 agcaagcacg gggtcacggt cgcggaggcg ccggcggccg ccgaggcgct ctcgcgcctg
     4741 gacgggctgg agtggctggg cctgatgacc atgggtccgc tctcaggcgg cgccgacgaa
     4801 acgcgggcgt gttttcgctc cctcgcgcgg ctcggcgcgc aactccgcgg cgtcaccggg
     4861 cgcccgctgc cggtattgag catgggcatg agcgacgatt tcgaggtcgc gatcgaagaa
     4921 ggcgcgacga tgattcgggt cggccggggt atcacaggcg agcgagtgta atcggacccg
     4981 ccgccgggtg agaaaaaact taccaggtgg tcggggttca tgtatgcttt ccgcgccatg
     5041 ggccttcggt gcctcgtaac cgattgacat ccaaatggtt acgggatgcg ccgggggagc
     5101 gcgtctggct cacggattgc actgcggcag gtattgccgc cgattgcgat cgacggggcg
     5161 gcgttccggt tcgcaggagg atcgacgatg cgtatcacac cgttggacgt tcgtaagcag
     5221 gagttccgta aggccatgcg cggtctcgat ggcgacgagg tgtacgcgtt cctctcgacc
     5281 gtcgcggacg agtacgaggc cgtgctcaac gacaacaagg cgctgcgcga gcgcctgctc
     5341 gagctcgacg acaaagtcca ggagtaccgc accatcgagc gcacgctgcg cgacacgctg
     5401 ttgaccgccg agcgcgtcac cgtcgaagcc aaggacaacg cgcgccgcga ggcgacgctg
     5461 atcgtcaagg aagcccagct cgaggcgcag aaggcgctgc gcgacatcac gacggaaggc
     5521 gcacgcctgc gccaagaggt ccagcgcctg cgctcgcagc gcgacgccta cattgccaag
     5581 atgaaggtgg tcgccgagtc gcacgtgaag ttcatcgaat ccgcggcccg cgacttccac
     5641 gtcgaagacc
//
LOCUS       DASPGK010000274         7325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1593000, whole genome shotgun sequence.
ACCESSION   DASPGK010000274 DASPGK010000000
VERSION     DASPGK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593000"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VEC56_07440"
     CDS             complement(<1..678)
                     /locus_tag="VEC56_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14023.1"
                     /translation="MLRARARRAARLVDAHRAGAVGAVLIGVIAIGGVFAPWLAPHDP
                     TVIAMDAALEPPSARHWFGTDAFGRDVLSRVMHGARFSLEVGVVSRLLALVLGTCLGL
                     FAGYYGGRVDQLLMRLADVTLAYPGLLLLIAVMAAVGPSKVALFLALGVVGWAGVARL
                     VRAQVLSLKEREFVTAIRSLGAANPRVIARHVFPNVLTPILVIFSMGLGASIMAESSL
                     SFLGLGAQ"
     gene            complement(714..1655)
                     /locus_tag="VEC56_07445"
     CDS             complement(714..1655)
                     /locus_tag="VEC56_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14024.1"
                     /translation="MLAYAFRRILWFVPILLGVATVTFLLLHVLPGDPVLSMVGERYD
                     AETLERLRAEMHLDAPLYERYARYLADLARFDLGTSYVTGVPVWESIRARFPHTLRLA
                     AAAMAIAVTFGVTIGVVAAWRRGSALDYLLVGGAVVGVSMPVFWLGVLLIYVFSMRLG
                     ILPPSGFGNGSIVYLVLPALTLAMASTAYIARITRSSVLDEIHETYVKAARAKGAGES
                     RVLVRHALRNALLPIITVVGVDFGSYLSGAVLTESIFAWPGLGRFTLDAIMKRDLPAI
                     QGAVLFMAILFMTVNLLVDLLYARVDPRVKFSREAQS"
     gene            complement(1707..3233)
                     /locus_tag="VEC56_07450"
     CDS             complement(1707..3233)
                     /locus_tag="VEC56_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV14025.1"
                     /translation="MRRAAVLCAVLLAGCGGGERTASPMLELALETAPNRLDPAFAVD
                     VAEGEICALVFDGLVGLSPDGVLSPALARAWETADGRRYVFHIDSRARFSDGRAVRAA
                     DVRASFERVLSPATASPRAWVLERIRGAAELRDGTASAVAGLEAPDDSTLVVELEEPF
                     APFLSMLALPAARVVDVASVGAGDVPMGSGPWVVSEWVRGDRVVLVPNPHHARRARGI
                     EGVRYRVIPEPFTRIAEFESGSIDVLEIPDAEVPRFLDDASRSSRLLKRPELRVFYLG
                     LNNVRYQDARVRRALNHAVNVPELSRVLQAGAAIPAHGAIPPGIPGHRERPGYEYDPV
                     RAKELLREAGAEDLKLEVWLRESAEGGRVMEAVQGYWRAVGVEATLVRREWSAFKQTV
                     SAGKVDAFLLDWFADYPDAENFLFPLFHSDNRGGGGNRSFFSDPNVDRMIDDASRILD
                     PHARAEACARIDSLVYAQAPWVYLYFPTTFHIVSERVTGYRLPRLYLANDFSDVRVSR
                     "
     gene            complement(3230..4513)
                     /locus_tag="VEC56_07455"
     CDS             complement(3230..4513)
                     /locus_tag="VEC56_07455"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXV14026.1"
                     /translation="MRTRRPRIIWPLAAVLAASAVSPAAAQPFGLFANAYLDSSRTTV
                     TRVTVEVPFRTLVFLKKEGYYDARYDAHISVRPAGQENAPATTYVLHGFATVKTYEET
                     RRRDQKSRSWRQIKLPPGDYVIDALLTIKNTSIGMRRSVTLRVPDFLASGLAFGTPDV
                     LSIPTPFTQTFARWQETAGALDRADTPPDAAIVGFDRQPAVRFALFVEGSGTGSVPCD
                     VFYEVVDLGDHQVAYGKRRVNLTGSSKDDEFVVTFNVDEWSPGLYRVNLRACTVEPQR
                     DATASVDIRIDVTRAMLGVDFDATLEVLGLIASDSDLKPLREAPESERAAAWAKFWAA
                     RDPDPSTLENEALVQHLSRVQYAMREFSQFGPGYRSDRGRVYIRYGPPEQIDTALDQR
                     AQGEYEIWRYYTQNRTFVFFDMFGVGDFKLVEGDL"
     gene            complement(4582..5829)
                     /locus_tag="VEC56_07460"
     CDS             complement(4582..5829)
                     /locus_tag="VEC56_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14027.1"
                     /translation="MQTPSRQNLWSENFAQAIEVMRAHRLRSALLILGVAIGIATVLM
                     MVTVLSGLSKKIYQDMASANRPYIYVQKFDFVVEGENAEELSRRADFTREDAAALART
                     CPSVDAAVFMIESQGTYSVRYGAEKTPPTTVLGSSHRLPELFSVTVDRGRFFTESEER
                     FNARVAVLAAGPAKDLFNQADPIGRYVMIGGAKYQVIGTMAPRNSIFGAWSDNFVCVP
                     HTTFGKDLHTERDWITLGATVRDDATLEQGVEEVTRALRVQRGLRPGQENDFSVETSE
                     SFLDLVKKVTVPIGIVLTIIASIGLLVGGIGVMNVMLISVTERTREIGVRRAIGAARG
                     DVMRQFLIEAGTLTGVGGVVGVVAGTALALGVSKLIHFPFVFSLPWTIVSLLFSVLVG
                     IGFGLYPARRAGDMDPVTALRHE"
     gene            complement(5842..7086)
                     /locus_tag="VEC56_07465"
     CDS             complement(5842..7086)
                     /locus_tag="VEC56_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14028.1"
                     /translation="MRPSFRVVLEGVRIAYTALLANKLRTLLTLLGNIVGIMSVIAVV
                     SLLGGIDLYMRNEVAAEGSNLFSIQRVNFLEAIEDFETFIDKVMYNPQLTRDDANALR
                     ESLELAKYVSARSESGGVNVSALGKSVEVTVAGCDAVYPFISEIPLTAGRHLTQIDDR
                     ENAQVAVLGWDVYSGLIAPRDAVGMTVRIGNRHFRVIGVAAQCGRILGQSRDRFVIVP
                     LGAYRKVFGPQQSVEIRIVAEDIRLLEDAMEEATVAMRIRHGLGPRDENDFAVTSSEQ
                     LINLWKSISRGVMTALVALVGVSMLVGGIVLMNTMVVSVTERTREVGLRKAVGAPQGA
                     IMWQFLVESATLSVFGGIAGMALGFAIAAIVSAVSVLPYVVNAALVGIAFAVTIAVGL
                     VFGTYPALRAARLDPVDALRSE"
     gene            complement(7090..>7325)
                     /locus_tag="VEC56_07470"
     CDS             complement(7090..>7325)
                     /locus_tag="VEC56_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14029.1"
                     /translation="RAATDEKPRDIEGVFVVEDGVARFRPVRVGIAGQSHFVVLSGLA
                     EGEQVVSGPFKTIGDIRDGQAVKIKKEDRRRRS"
BASE COUNT         1314 a         2558 c         2410 g         1043 t
ORIGIN      
        1 ctgcgcgccc aggcccagga aggagagcga cgactcggcc atgatcgacg cacccagtcc
       61 catcgagaag atcaccagga tgggcgtcag cacgttgggg aacacgtggc gcgcgatcac
      121 gcgcgggttg gccgccccca gcgagcgaat cgcggtcacg aactcgcgct ctttgagcga
      181 cagcacctgc gctcgcacga ggcgtgccac gcccgcccac cccaccacgc cgagggccag
      241 gaacagtgcg accttgctcg ggcccacggc cgccatcacc gcgatcagga gcaagagacc
      301 cggatacgcc agcgtgacgt cggcgagccg catcaatagc tggtcgacgc gacccccgta
      361 atagcccgcg aacaggccca ggcaggtccc caagacgagc gccaacaacc gcgaaaccac
      421 gcccacttcg agcgagaagc gcgcgccgtg catgacccgg ctcagcacat cgcggccgaa
      481 ggcgtcggta ccgaaccagt ggcgcgcact cggcggctcg agcgccgcgt ccatcgcgat
      541 cacggtggga tcgtgggggg cgagccaggg cgcaaaaaca ccgccgatcg cgatcacgcc
      601 gatcagcacg gcacccaccg cacccgcgcg atgggcgtcg accagacgcg ccgccctgcg
      661 ggcgcgggcg cgcagcacgt ccgctgcgga tctccgcgtg ggctgcgacg agctcacgac
      721 tgcgcctccc gcgagaactt cacgcgcgga tcgacgcgcg cgtacaggag atccaccagc
      781 agattgacgg tcatgaacag gatggccatg aacagcaccg caccttggat ggcgggcagg
      841 tcgcgcttca tgatcgcatc gagggtgaat cgtcccaggc cgggccacgc gaagatcgac
      901 tcggttagca ccgcgccgct caggtagctc ccgaagtcga cgcccaccac cgtgatgatc
      961 ggcagcagcg cgttgcgcag cgcgtggcgc acgagcacgc ggctctcccc cgcccccttg
     1021 gcgcgcgccg ccttgacgta cgtctcgtga atctcgtcca agacgctcga gcgcgtgatg
     1081 cgcgcgatgt atgcggtcga cgccatcgcc agggtgagcg cgggcagcac cagataaacg
     1141 atgctcccgt tgccgaaacc ggacggcggc aggattccga ggcgcatcga gaagacgtag
     1201 atcagcagca cacccagcca gaacacgggc atggagacgc ccaccaccgc cccgcccacc
     1261 aacagatagt cgagcgcgga accgcgtcgc cacgccgcca caacaccgat ggtgaccccg
     1321 aacgtgaccg cgatcgccat cgccgcggcc gccagacgca gcgtgtgcgg gaaacgcgcg
     1381 cgaatcgact cccacaccgg gaccccggtg acgtacgacg tccccaggtc gaagcgcgcc
     1441 aggtccgcca ggtagcgcgc gtaacgctca tagagcgggg cgtcgaggtg catctccgcg
     1501 cgcaggcgct cgagcgtctc cgcgtcgtag cgctcgccca ccatggaaag caccgggtcc
     1561 cccggcagga cgtggaggag cagaaacgtc accgtcgcca cgccgaggag gatgggaacg
     1621 aaccacagaa tgcgtctgaa tgcgtacgcg agcatgcgtg ggcgaggata ggccaaggcc
     1681 cgcgcgcgct gcaatctcgc gtttggctag cgggagacgc gaacgtcgga gaaatcgttg
     1741 gcgaggtaga gccgcgggag ccggtaaccg gtcacgcgct cggagacgat gtggaaggtg
     1801 gtgggaaagt agagatagac ccagggcgcc tgcgcgtaca cgagactgtc gatgcgcgcg
     1861 cacgcctccg cgcgtgcgtg cggatcgagt atccggctcg cgtcgtcgat catgcggtcg
     1921 acgttggggt ccgaaaagaa cgagcggttg ccgccgccgc cacgattgtc ggaatgaaac
     1981 aagggaaaca ggaagttctc cgcgtcggga tagtccgcga accaatcgag caggaacgcg
     2041 tcgaccttgc ccgcactcac ggtctgcttg aacgcactcc actcgcgccg caccagcgtc
     2101 gcctcgaccc cgaccgcgcg ccagtagcct tgcaccgcct ccatcacgcg accgccttcg
     2161 gcgctctcgc gcagccacac ctcgagcttc aggtcctcgg cgccggcttc gcgcaggagt
     2221 tccttggccc gcaccgggtc gtactcgtag cccggccgct cgcggtggcc cggaatcccg
     2281 ggcggaatgg cgccgtgcgc ggggatcgcg gctcccgcct gcagcacgcg cgagagctcg
     2341 gggacgttga cggcgtggtt gagggcgcgt cgcacgcgcg cgtcttgata gcggacgttg
     2401 ttcaacccga ggtagaacac gcgcagctcc gggcgcttca acaagcggct cgagcgcgac
     2461 gcatcgtcca agaaacgcgg cacctccgcg tccggaatct cgagcacatc gatggacccg
     2521 gattcgaact cggcgatgcg cgtgaacggc tcgggaatca cgcgatagcg cacgccctcg
     2581 atcccgcgcg cgcgccgggc gtggtgcggg ttgggaacga gcaccacgcg atcgccccgc
     2641 acccactccg agaccaccca cgggcccgag cccatcggga cgtcgcccgc gcccacgctc
     2701 gcgacgtcga ccacgcgcgc ggcgggcagc gccagcatcg acaggaaggg cgcgaagggc
     2761 tcctcgagct cgaccaccag cgtcgaatcg tccggtgctt cgagccccgc gaccgcggac
     2821 gccgttccgt cgcggagttc ggcggcgccg cgtattcgtt ccagcaccca cgcgcgcggc
     2881 gacgcggtgg ccggcgatag cacgcgctcg aagctcgcgc gcacgtcggc ggcgcgcacc
     2941 gcccgcccgt cggaaaatcg cgcgcgcgaa tcgatatgga acacgtagcg ccggccgtcc
     3001 gcggtctccc acgcgcgcgc gagcgcgggc gagagcacgc cgtcgggaga gagacccacc
     3061 aggccgtcga aaacgagcgc gcagatttcg ccctccgcga cgtccaccgc gaacgcgggg
     3121 tccagccggt tgggagcggt ctcgagcgcg agctcgagca tcggagatgc cgttcgctcg
     3181 ccgccgccgc agccggctag aagaacggcg cacaacaccg ccgcgcgtct cacagatcgc
     3241 cctccaccag cttgaagtcg cccaccccga acatgtcgaa gaacacgaag gtccggttct
     3301 gggtgtagta gcgccagatc tcgtactccc cttgcgcgcg ctggtcgagc gcggtatcga
     3361 tctgctcggg cggtccgtag cggatgtaca cgcgcccgcg atccgagcga taacccggtc
     3421 cgaattgcga gaactcgcgc atcgcgtact ggacgcgcga caggtgctgg acgagagcct
     3481 cgttctccag cgtgctcggg tcggggtcgc gggccgccca gaacttggcc cacgccgccg
     3541 cgcgctccga ttcgggcgcc tcgcgcagcg gtttgagatc gctgtcgctc gcgatcagcc
     3601 ccaacacctc cagggtggcg tcgaaatcca cccccagcat cgcgcgcgtg acgtcgatcc
     3661 ggatgtcgac cgaggcggtc gcgtcgcgct gcggctcgac ggtgcacgcg cgcaggttga
     3721 cccgatacag ccccggcgac cactcgtcga cgttgaaggt cacgacgaat tcatcgtcct
     3781 tggaagatcc cgtcagattc acgcgccgct tgccgtacgc gacttggtga tcgcccaagt
     3841 cgaccacttc gtagaaaacg tcgcatggca ccgatcccgt gccgctgcct tccacgaaca
     3901 acgcgaagcg aaccgccggc tggcggtcga atcccacgat ggccgcgtcc gggggcgtgt
     3961 cggcgcgatc gagcgcgccg gcggtctcct gccagcgcgc gaaggtctgc gtgaacggcg
     4021 tcggtatcga aagtacgtcc ggcgtcccga aggcgaggcc ggaggcgagg aagtccggaa
     4081 cgcgcagcgt caccgaccgg cgcatgccga tactcgtgtt cttgatcgtg agcaaggcgt
     4141 cgatcacgta gtcgcccggc ggcaacttga tctgccgcca cgagcgcgac ttctggtcgc
     4201 gtcgccgcgt ctcctcgtag gtcttgacgg tggcgaaacc gtgcagcacg tacgtggtcg
     4261 ccggtgcgtt ctcctgcccc gcgggccgca ccgagatgtg cgcgtcgtag cgggcgtcgt
     4321 agtacccctc tttcttgagg aagacgagcg tgcggaacgg cacctcaacg gtgacgcgcg
     4381 tcacggtggt gcggctggaa tcgagatacg cgttggcgaa caggccgaaa ggttgcgcgg
     4441 ccgcgggcga caccgccgag gcggcgagca cggcggcgag cggccaaatg atcctcggtc
     4501 ggcgtgtgcg catggtgtcc tgggaagcgg ggacgagggg aacttaggct accgcgccgt
     4561 gcgggagcaa gcaggaaccc gtcattcgtg ccgcaatgcg gtcaccgggt ccatgtcccc
     4621 ggcgcggcga gcgggataga gcccgaagcc gatccccacc aggaccgaga acaggagcga
     4681 cacgatcgtc cacgggagcg agaagacgaa cgggaagtga atgagcttgg agacaccgag
     4741 cgcgagtgcg gtaccggcta cgacgcccac gaccccgccc acacccgtca gcgtccccgc
     4801 ctcgatcaag aactgccgca tcacgtcccc gcgcgcggca ccgatggcgc ggcgcacgcc
     4861 gatctcgcgc gtacgctcgg tcaccgagat cagcatgacg ttcatgacgc cgatgccgcc
     4921 caccaacaag ccgatcgatg cgatgatggt gagaacgatc ccgatcggca ccgtgacctt
     4981 cttgaccaaa tcgaggaagg actcgctcgt ttccaccgag aagtcgttct cctggcccgg
     5041 gcgtaagccg cgttggacgc gcagcgcgcg tgtcacctct tcgacgcctt gctcgagggt
     5101 ggcgtcgtca cgaaccgtcg cgcccagcgt gatccagtcg cgctcggtgt ggaggtcctt
     5161 gccgaaggtg gtgtgcggca cgcacacgaa gttgtccgac cacgcgccga agatcgagtt
     5221 gcgcggcgcc atggtcccga tcacttggta cttggcaccc ccgatcatca cgtaacgccc
     5281 gatggggtcg gcctggttga acaggtcctt ggccggcccg gccgccagca ccgcgacgcg
     5341 cgcattgaag cgctcctcgc tctcggtaaa gaagcgtccg cgatccaccg tgaccgagaa
     5401 cagctccggg agtcggtgcg acgagcccag gaccgtcgtg ggcggggtct tctcggcgcc
     5461 atagcgaacc gaataggtcc cttgggattc gatcatgaac acggcggcgt cgacgctggg
     5521 gcaggttcgc gccagcgcgg ccgcgtcttc gcgcgtgaag tcggctcgcc gcgacagctc
     5581 ctccgcgttc tccccttcca ccacgaagtc gaacttctgg acgtagatgt agggacggtt
     5641 ggcggacgcc atatcctggt agatcttctt gctcaagccc gagagcacgg tcaccatcat
     5701 gagcacggtg gcgatcccga tcgcgacccc gagaatgagc agcgccgagc gcagccggtg
     5761 cgcgcgcatc acctcgatcg cttgcgcgaa gttctcgctc caaagattct gccgcgaagg
     5821 tgtctgcatc gccttggtgg gttattccga gcgcagcgca tccaccggat cgaggcgcgc
     5881 ggcgcgcagc gccgggtacg tcccgaacac gagcccgacc gcgatcgtca ccgcgaacgc
     5941 gattcccacc agggccgcgt tgacgacgta gggcagcacc gacaccgccg acacgatggc
     6001 ggcgatcgcg aagccgagcg ccatgccggc gataccgccg aacaccgaga gcgtggccga
     6061 ctcgaccagg aactgccaca tgatcgcccc ctggggggcg cccaccgcct tgcgcagccc
     6121 gacttcgcgc gtgcgctcgg tcaccgagac caccatcgtg ttcatcagca cgatgccgcc
     6181 caccagcatc gaaacgccga ccagcgccac cagcgccgtc atcacgccgc ggctgatcga
     6241 cttccacaga ttgatgagct gctcgctgga cgtgaccgcg aagtcgttct cgtcgcgcgg
     6301 gcccaggccg tgccgtatgc gcatcgcgac cgtcgcctcc tccatggcgt cttcgagcag
     6361 gcgaatgtcc tcggccacga tgcgaatttc caccgactgc tggggcccga acaccttgcg
     6421 gtacgcgccc agcggcacga tcacgaagcg gtcgcggctc tggccgagga tacggccgca
     6481 ctgcgccgcc acgccgatca cgcggaagtg acggttgccg atgcgaaccg tcatccccac
     6541 cgcgtcgcgc ggcgcgatca gtccgctgta cacgtcccag cccaggaccg cgacttgcgc
     6601 gttctcgcgg tcgtcgatct gggtcaggtg gcgacccgcg gtcaacggaa tctcgctgat
     6661 gaagggatag acggcgtcgc agcccgcgac cgtcacctcg acgctcttgc ccagggccga
     6721 cacgttgacc ccgcccgact cggagcgcgc gcttacgtac ttggcgagct cgagcgactc
     6781 gcgcagcgcg ttggcgtcgt cgcgcgtgag ctgcgggttg tacatcacct tgtctatgaa
     6841 ggtctcgaag tcttcgatag cttcgaggaa gttgacgcgc tgaatactga agagattgct
     6901 gccctcggcg gcgacctcgt tgcgcatata gaggtcgatc ccgcccagga gcgaaaccac
     6961 cgcgatcacc gacatgatgc cgacgatatt gccgagcagc gtcaagagcg tgcgcagctt
     7021 gttggcgagg agcgcggtgt aggcgatgcg cacgccttcg aggacgacgc ggaacgatgg
     7081 acgcacgcgc tacgacctcc gcctgcggtc ttccttcttg atcttgacgg cctggccgtc
     7141 gcggatgtcg ccgatggtct tgaacggccc cgacaccacc tgctcgcctt cggccaggcc
     7201 ggacaggacg acgaaatggc tctgccccgc gatccccacc cgcaccggcc ggaagcgcgc
     7261 gactccgtct tccacgacga agaccccttc gatgtcgcgc ggtttctcgt cggtcgccgc
     7321 gcgac
//
LOCUS       DASPGK010000275         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_890674, whole genome shotgun sequence.
ACCESSION   DASPGK010000275 DASPGK010000000
VERSION     DASPGK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890674"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VEC56_07475"
     CDS             <1..140
                     /locus_tag="VEC56_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXV14030.1"
                     /translation="AFEPRLILPLSLSYDHRLIDGAAAARFLRWVCEALEDPILLALE
                     G"
     gene            148..1566
                     /gene="lpdA"
                     /locus_tag="VEC56_07480"
     CDS             148..1566
                     /gene="lpdA"
                     /locus_tag="VEC56_07480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXV14031.1"
                     /translation="MAANETFQLVVLGGGPGGYAAAFLGADLGLKTALVDVEENPGGV
                     CLYRGCIPSKALLHVAKVLHEADAARAMGVEFGEPQINIDAVRAWKDGVVKKLTSGVG
                     QVAKFRKVTYVRGFGTFKDAHTLSVALAAGGTQDLRFDHCIVATGSRPATIPFFKPDS
                     PRVLDSTSALDLRDVPESMLVVGGGYIGLELGSVYASLGARVSVVEMMPSLLPGADRD
                     LASVLEKRIAKSFERIMVNTKVVEAHETGDGINVTFDGAVKESLTYDAVLVSVGRKPN
                     SIGFGLEKTKARVNERGFVEVDAQRRTHEPSIFAIGDVVGEPMLAHKASHEGRTAAEV
                     IAGHKVAFEPAAIPAVIFTDPEIAWCGLTETQAKEGSIDVAVAKFPWGASGRAMSIER
                     TDGLTKLVIDPKTERVLGVGIAGVGAGDMISEGVLAIEMGATAADIKLTIHPHPTLSE
                     TLMEAAEVFYGQSTHVYRPKRS"
     gene            1613..2869
                     /locus_tag="VEC56_07485"
     CDS             1613..2869
                     /locus_tag="VEC56_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14032.1"
                     /translation="MARLPLMAVLTLVAVSVANTSNARTFVLPHVIESSGDASNTSFA
                     FDTSFQAAYTAGLVDGEPVSNATVNVYLLNAAGAPVVSATANPVCNPCVFVLDSATRR
                     VSAAFQTLIESAGGFAGGVVLGFALFEVTGDDDHVDIGATVVNSHTSPFDLDVAFPDV
                     VELPGGLDPSLRLIVFPSVVESSTGTVNALNAFDTIFYAVYGGGVAGSSIPAGAGATV
                     SLYLFNDNGTLKQSQIGNNVCAPCAQVVSAGSPKATFSLQNLFIGAGGFAFGVEVGFA
                     LVSISGTADAVGLQGFFLNSHTSPFDLSTASLEAREVPTSSPPTGVDSPPALDSLLRS
                     YPNPLQGATRIDYTVPSAGEASIEIVDVTGRVVARPVRGPHTSGEHFLDWDGRGEDGR
                     ALPSGVYFARLATDAGVRTLKLTLVR"
     gene            2883..>3167
                     /locus_tag="VEC56_07490"
     CDS             2883..>3167
                     /locus_tag="VEC56_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXV14033.1"
                     /translation="MNDLLRIAIVVAVIVFLLGIRIVRTTRRALVERLGKYHHFANPG
                     FNWIIPVIDRLYLVNITEQMVDAEPQEIITNDNLNALVDAQIYFKIKDDAE"
BASE COUNT          539 a         1043 c         1045 g          540 t
ORIGIN      
        1 gcgccttcga gccgcggctg atactcccgc tctcgctctc gtacgaccac cgcttgatcg
       61 acggtgccgc cgccgcgcgc ttcctgcgct gggtgtgcga agcgctcgaa gatcccatcc
      121 tgctcgcact cgaaggctaa gctctcgatg gccgccaacg agaccttcca gctcgtcgtc
      181 ctgggcggcg gtccgggcgg ttacgcggcc gcgtttctgg gcgccgatct cgggttgaag
      241 acggccctcg tcgacgtcga agagaacccg gggggcgtgt gcctctatcg ggggtgcatt
      301 ccgtcgaagg cgctcttgca cgtcgcgaag gtcctccacg aagcggacgc agcgcgcgcg
      361 atgggcgtgg agttcggcga accgcaaatc aacatcgacg cggtgcgggc gtggaaagac
      421 ggtgtggtca agaagctcac gagcggtgtc gggcaggtgg cgaagttccg caaggtcacc
      481 tacgtgcgag gcttcgggac gttcaaagac gcgcacacgc tgtcggtggc actagccgcg
      541 ggcggcaccc aagacctacg gtttgaccac tgcatcgttg cgaccggctc gcgtcccgcg
      601 accattccgt tcttcaagcc cgactcgccg cgcgtgctgg actccacgag cgccctcgat
      661 ttgcgcgacg tgcccgagtc gatgctggtg gtgggcggcg gctacatcgg cctcgagctg
      721 gggagcgtgt acgcaagcct cggcgcccgg gtctccgtgg tcgaaatgat gccgagcctc
      781 ctgccgggcg ccgatcggga tttggcttcg gtgctggaga agaggatcgc gaagagcttc
      841 gagcggatca tggtcaatac gaaagtggtc gaggcgcacg agaccggcga cggtatcaac
      901 gtcaccttcg acggcgcggt caaagaatcg ctgacgtacg acgcggtgct cgtttcggtc
      961 ggccgcaaac ccaactcgat cggcttcgga ctcgagaaga ccaaggcgcg cgtcaacgag
     1021 cgcggattcg tggaagtcga cgcccaacgc cgcacccacg aacccagcat cttcgccatc
     1081 ggcgacgtgg tgggcgagcc catgctcgcg cacaaggcct cgcacgaagg gcgcaccgcg
     1141 gccgaggtga tcgccgggca caaggtcgcc ttcgagcccg ccgcgatccc ggccgtcatt
     1201 tttacggatc ccgagatcgc gtggtgcggc ttgaccgaga cccaggccaa ggagggcagc
     1261 atcgacgtcg ccgtcgccaa gtttccctgg ggtgcgtccg gacgcgccat gtcgatcgag
     1321 cgcaccgacg ggttgaccaa gctcgtcatc gaccccaaga ccgagcgcgt gctgggggtg
     1381 ggtatcgcgg gcgtgggcgc cggtgatatg atctccgaag gcgtgctcgc gatcgagatg
     1441 ggtgcgacgg cggccgacat caagctcacc atccaccccc acccgacgct gtccgaaacg
     1501 ctcatggaag cggcggaagt cttctacggt cagagcaccc acgtgtaccg gcccaaacga
     1561 tcgtagctcc ccgaacccgt ggtccgcgaa cgcggacgcg aaaggagccc ccatggctcg
     1621 cctcccgttg atggcagtgc tgaccctcgt tgccgtctcc gttgccaaca cgagcaacgc
     1681 gcgcaccttc gtcttgcccc acgtgatcga gtccagcggc gacgcgtcga acacgagctt
     1741 cgcgttcgac acgtcgttcc aggccgcgta cacggcgggg ctggtcgacg gcgagcccgt
     1801 gtcgaacgcc accgtgaacg tgtatttgct caacgcggcc ggtgctcccg tcgtgagtgc
     1861 gacggcgaat ccggtgtgca acccgtgcgt gttcgtgctc gactcggcga cgcgccgggt
     1921 gagcgccgcg ttccagacgc tgatcgagtc ggcgggcgga ttcgcgggag gggtcgtact
     1981 cgggttcgcc ctgttcgaag tcaccggcga cgacgaccac gtcgacatcg gcgcgacggt
     2041 ggtgaactcg cacacgagcc cgttcgacct ggacgtcgcc tttcccgacg tggtggagct
     2101 tcccggcggg ttggatccgt cgctgcgctt gatcgtattc cccagcgtgg tggaatcatc
     2161 gaccggcacg gtcaatgcgc tcaacgcgtt cgacacgatc ttctatgccg tatacggcgg
     2221 tggtgtcgcc gggagctcga tacccgcagg agccggtgcg acggtatcgc tctatctgtt
     2281 caacgacaac ggcacgctga aacagagcca gatcggcaac aacgtctgcg ccccgtgcgc
     2341 gcaggtggtg agtgcgggca gccccaaggc gacgttcagt ctgcagaacc tgttcatcgg
     2401 cgcgggcggc tttgctttcg gcgtcgaggt cggcttcgcc ctcgtgtcca tcagcgggac
     2461 ggcggacgcg gtgggtttgc agggcttctt cctcaactcg cacacgagtc cgttcgacct
     2521 gtcgaccgcc tcgctggagg cgcgggaggt accgacgtcg tcaccgccca ccggggtcga
     2581 ttcacccccc gcgctcgact cgctcttgcg cagctacccc aatccgctcc agggcgcgac
     2641 gcggatcgac tacaccgtgc cgagcgccgg cgaggcctcc atcgagatcg tcgacgtaac
     2701 cggaagggtg gtggcgcggc cggtgcgagg tccgcacacc tctggcgagc acttcctgga
     2761 ctgggacgga cgcggagaag acggtcgcgc gcttcccagc ggagtctact ttgcccgtct
     2821 ggcgacggac gccggcgtgc gcacgctcaa gctaacactg gtacgctagc agagagggaa
     2881 tcatgaacga cctgcttcga atcgcgatcg tcgtggcggt aatcgtcttt ctgctcggca
     2941 tccgcatcgt gcgcaccacg cggcgcgcgc tggtggagcg cctgggcaag taccaccact
     3001 tcgccaaccc cggcttcaac tggatcattc ccgtgatcga ccgcctctac ttggtcaaca
     3061 tcaccgagca gatggtcgac gccgagccgc aggagatcat caccaacgac aacttgaacg
     3121 cgctggtcga cgcgcagatc tatttcaaga tcaaggacga cgccgag
//
LOCUS       DASPGK010000276         6986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1241541, whole genome shotgun sequence.
ACCESSION   DASPGK010000276 DASPGK010000000
VERSION     DASPGK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241541"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="rpsB"
                     /locus_tag="VEC56_07495"
     CDS             <1..366
                     /gene="rpsB"
                     /locus_tag="VEC56_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXV14034.1"
                     /translation="AQFSKKEISGFSKERERILKTLGGVRNMTDLPGLVFIVDTKKEQ
                     NAVNEARRLRIPIVGVVDSNADPGEVDYPVPGNDDAMRAIGLFAKVVADACIEGRSRF
                     TEGRDVSSGDEPESAKSVA"
     gene            397..990
                     /gene="tsf"
                     /locus_tag="VEC56_07500"
     CDS             397..990
                     /gene="tsf"
                     /locus_tag="VEC56_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXV14035.1"
                     /translation="MEISANLVKNLRERTGAGMMDCKKALEESAGDVEKAIEFLRIRG
                     LSKAAKKAGRETSEGLVVSYIHPGNRIGVLLEVNCETDFVARTDDFQNLVKNLAMHIA
                     AAAPLGVTREDIPADLLAKEKEVFRAQALEEGKPAAVVDKIVQGRIDKFYAEAALMDQ
                     VYVKDNDKKVSDLLGEAIAKLGENIKVARFARFQLGQ"
     gene            1010..1720
                     /gene="pyrH"
                     /locus_tag="VEC56_07505"
     CDS             1010..1720
                     /gene="pyrH"
                     /locus_tag="VEC56_07505"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXV14036.1"
                     /translation="MNRLRFQRILLKFSGEILARDESGGIDFDKLADYSKQIAEVKAL
                     GTEIGIVIGGGNILRGNSDEHGIDRVTADYMGMLATVINALAFQSALERIGVDTRVLT
                     AIKMEQLAEPYIKRRAIRHLEKGRVVIFAGGTGNPYFTTDTAAALRAIEIEADVVMKG
                     TKVDGVYDGDPMKDAGAKRFESLAYIDVLNRRLRVMDATAISLCMENRLPIVVFKLTG
                     ENNLLRVVRGEPVGTIVS"
     gene            1740..2297
                     /gene="frr"
                     /locus_tag="VEC56_07510"
     CDS             1740..2297
                     /gene="frr"
                     /locus_tag="VEC56_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015562568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXV14037.1"
                     /translation="MLDEQYKKTEEHMKKTLELLVHELAGIRTGKASPAILDTIKVNY
                     YGQHVPVKQVANIAVPDARMITIQPWDRSLVHEIEKAIQASELGLNPQNDGGTIRLPI
                     PQLTEERRKDLVKVVKRIGEDSKVAVRNVRRDTNERIKKLEKEHDISEDEMRAKQDAI
                     QKLTDAYVKKVDEAVAAKEKEILEL"
     gene            2294..3043
                     /locus_tag="VEC56_07515"
     CDS             2294..3043
                     /locus_tag="VEC56_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXV14038.1"
                     /translation="MSHDLDAEIESLKGAENLPRHIAVIMDGNGRWAKKRRLPRLAGH
                     RAGTEPVRKCVRTSARIGIEYLTLYTFSLENWSRPKAEVAGLMVFLEEVLRRETLELD
                     ENGVRLNAIGRLDLLPAATRAALDRSIDKLSKNRGLTLTLALSYGGRAEIVDAARRVC
                     EDVAAGRLGAQDIDEERFRERLYDPSVPDPDLLVRTSGELRISNFLLWQIAYTEIWVT
                     DVLWPDFTEMDLIAGIRAYQGRDRRYGLHST"
     gene            3057..3944
                     /locus_tag="VEC56_07520"
     CDS             3057..3944
                     /locus_tag="VEC56_07520"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXV14039.1"
                     /translation="MHPHPKRSAAAPAPAKPRGTRRTAIERVVAGVVFVPLFIIITRA
                     GGYAFLALIDLVIVLGMLEFYAMMRAKGMHPYRGIGVASGITLSTYIFFRSGQYANFV
                     FTFILIALMGLELARKDNRRAAYHVSTTIFGVIYVAYLSSHFVLLRELPNAVGVPYSQ
                     GASFVFLVVAVTWASDTGAYTIGSLFGSHTLLPRVSRHKTWEGALGGVLFGAVAGWIS
                     SVSFAGYLKAWEGVVIGILSSLVGLLGDLFESMLKRDADIKDTSNLIPGHGGVLDRFD
                     SLLFTAPLIYYFLKFVIFQ"
     gene            3988..5157
                     /gene="dxr"
                     /locus_tag="VEC56_07525"
     CDS             3988..5157
                     /gene="dxr"
                     /locus_tag="VEC56_07525"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXV14040.1"
                     /translation="MKRIVVLGSTGSVGRNVLDVVRRHPEQFRVVGLAAHRNVDVLRA
                     QCADHPDARFVVTDAAAAAEMANDPARRAGLAGVGEPALLDLVRGSGADLVVNALVGF
                     VGLAPTLAALEAGIPVAIANKESIVAGGELLLRAARASGVELVPIDSEHVAIAQCLAG
                     AARADVERVILTASGGALRDRNPASFAEVTVRDVLAHPTWRMGAKITVDSATLVNKGL
                     EIIEAHWLFGLPYEKIDVVIHPQSIVHSVVRFVDGSMIAQMATPDMRIPILYALAYPN
                     RLKSDLGADLLEFPALSFAPVDAHRYPCFPIAVAAAKAGGTAPTVLSAVNEVAVEAFL
                     AGRLAYTAIPEWIASALEALPARPVTSLDDVVAADGEARRWIREHHTTASRGDGR"
     gene            5173..6555
                     /gene="rseP"
                     /locus_tag="VEC56_07530"
     CDS             5173..6555
                     /gene="rseP"
                     /locus_tag="VEC56_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXV14041.1"
                     /translation="MLTTILAGLFVLGVVIIVHEFGHFIVAKLAGVYVKVFSVGFGKR
                     ILRWRRGETVYAISALPFGGYVKFAGESDLTEVPAPDPETPPGPLDETPDSEIPRERY
                     FTTQRKLIRAAILLAGPFMNYLLAVVLFVGMYFAQGVSVVPTTRVGQVTADGPAALSG
                     IAPGDTIASIDGARVEDWYDIESLLTDDPSERKAILVRRDGHEREITFEPKVEGRHVS
                     LGMFAFTSSRVGRVQKNGPAYRAGLENGAVIEAINDTLVTEYADLVRIINANADNPLY
                     VRWTMDGLAHADTIVPEPKQVLKAGSTSELTTVGIIGIHPYAERRREGLFTSIGNGFY
                     AANETTAQIVGYLGLLFTGRMGVTTLGGPITITQMAGDVANWGFDYLLGFLAFFNINL
                     CVFNLIPLLPFDGGHLAILAIEGVMRRPVNRRLRDWLTQGGFVLIILLMAFVLVLDLS
                     RCAGSTPSGF"
     gene            complement(6535..>6986)
                     /locus_tag="VEC56_07535"
     CDS             complement(6535..>6986)
                     /locus_tag="VEC56_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14042.1"
                     /translation="MVPAHAHALTPLRRRVYLTSDHCRLPMTARDVRPIVPRVKLRPL
                     VVAAVLVLFAACSAKEKPADPGLSPDEVYLVESYVRVRRAGAMFPHQRVLADSLLDRM
                     AGEIDTVRVARTTAALNASPERWALVFQAIEARMGDPRAQPSESTRS"
BASE COUNT         1229 a         2300 c         2307 g         1150 t
ORIGIN      
        1 gcgcagttct ccaagaagga gatctccggc ttctcgaagg aacgcgagcg catcctgaag
       61 acgctgggcg gtgtgcgcaa catgaccgac cttcccggtc tggtgttcat cgtcgacacc
      121 aagaaagagc agaacgcggt caacgaggcg cgccgcttgc gcatcccgat cgtgggtgtg
      181 gtggacagca acgccgaccc gggcgaagtg gactatccgg ttccgggcaa cgacgacgcc
      241 atgcgcgcca tcggcctgtt cgccaaagtg gtggccgacg cgtgcatcga aggccgctcg
      301 cgctttaccg aagggcgcga cgtctcgagc ggcgacgagc ccgagagcgc caagtcggtc
      361 gcgtagttcc agaatagaca ggacatggag agaacgatgg aaatcagtgc gaacttggtg
      421 aagaatctgc gcgagcgtac cggcgcgggc atgatggact gcaagaaggc gctcgaggag
      481 agcgcgggcg acgtcgagaa ggcgatcgag ttcctgcgca ttcgcgggct gtcgaaggcc
      541 gccaagaagg ccggccgcga gacctcggaa ggactggtcg tgtcgtatat ccacccgggc
      601 aaccgcatcg gcgtgctgct cgaggtcaat tgcgagaccg acttcgtcgc gcgtacggac
      661 gacttccaga acctggtcaa gaacctggcc atgcacatcg ccgcggccgc cccgctcggg
      721 gtgacgcgcg aggacatccc ggccgacctc ttggccaagg aaaaggaagt gttccgggcg
      781 caggccctcg aagagggcaa gccggcggcg gtggtcgaca agatcgtgca ggggcgcatc
      841 gacaagttct acgcggaagc cgctctcatg gaccaggtgt acgtcaagga taacgacaag
      901 aaggtctccg acctcctcgg cgaagcgatc gccaagctgg gcgagaacat caaggtcgcg
      961 cggttcgcac gcttccagtt gggccaatag tcttccaggg ggtgccgttc tgaaccgctt
     1021 gaggttccag aggattctgc tcaagttcag cggcgagatt ctcgcgcgcg acgagagcgg
     1081 cggcatcgat ttcgacaagc tcgccgacta ctcgaagcag atcgccgagg tcaaggccct
     1141 cggcaccgag atcggcatcg tcatcggcgg cggtaacatc ctgcgcggga attccgacga
     1201 acacgggatc gaccgcgtca ccgccgacta catgggcatg ctggcgacgg tgatcaacgc
     1261 gctcgcgttc cagagcgcgc tcgagcgcat cggcgtggac acgcgcgtgt tgaccgccat
     1321 caagatggag cagctcgcgg agccctacat caagcgccgc gccatccgtc acctcgagaa
     1381 gggccgcgta gtcatcttcg ccggcggcac cggcaatccc tacttcacca ccgacaccgc
     1441 ggccgccctg cgcgccatcg agatcgaagc cgacgtcgtc atgaagggca ccaaggtcga
     1501 cggcgtctac gacggcgacc ccatgaagga cgccggcgcc aagcgcttcg agtccctcgc
     1561 ctatatcgac gtccttaacc gccgtttgcg ggtgatggat gcgaccgcca tctccctgtg
     1621 catggaaaac cggttgccga tcgtggtgtt caagctcacg ggcgagaaca acctgctgcg
     1681 cgtggtgcgc ggcgagccgg tcggcaccat cgtgtcgtag ctcgctagga ggctttgaca
     1741 tgttggacga gcagtataag aagaccgaag agcacatgaa gaaaacgctc gagctcctgg
     1801 tgcacgagct ggccgggatt cggaccggca aggccagccc cgccatcctc gacaccatca
     1861 aggtgaacta ctatggccaa cacgtgccgg tgaagcaagt ggccaacatc gcggtgccgg
     1921 acgcgcgcat gatcacgatc cagccctggg accgctcctt ggtccacgag atcgagaagg
     1981 cgatccaggc ctccgagctg gggttgaacc cgcagaacga cggcggcacc attcgcctcc
     2041 cgattccgca gctcaccgag gagcggcgca aggacctggt caaggtggtc aagcgcatcg
     2101 gcgaggactc caaggtggcg gtgcgaaacg tgcgacgcga caccaacgag cgcatcaaga
     2161 agctcgaaaa agaacacgac atctccgaag acgagatgcg cgccaagcag gacgccattc
     2221 aaaagctgac cgacgcgtac gtcaagaagg tggacgaagc cgtcgcggcc aaggaaaagg
     2281 aaattctcga gctgtgagcc acgacctcga cgcagagatc gagtcgctca agggcgcgga
     2341 aaatctaccc cgacacatcg ccgtcatcat ggacggcaac ggccgctggg ccaagaagcg
     2401 gcgcctcccg cgcctggccg gccaccgggc cggcaccgaa cccgtccgca agtgcgtgcg
     2461 cacctcggcg cgcatcggga tcgaatacct caccctctat acgttctcgc tcgaaaactg
     2521 gagccgcccc aaggccgaag tggcgggcct gatggtgttc ctcgaggagg tgctgcggcg
     2581 cgagaccctc gagctggacg agaacggcgt gcgcttgaac gccatcgggc gactcgacct
     2641 tctgccggcc gcgacccgcg ccgcactcga tcgttccatc gacaagctgt ccaagaaccg
     2701 cgggctcacg ctgacactgg ccttgagcta cggcggacgc gcggagatcg tcgacgcggc
     2761 gcgccgcgtg tgcgaggacg tcgcggcggg aaggctgggc gcgcaggaca tcgacgagga
     2821 gcgtttccgg gagcgactct acgacccctc cgttccggac ccggacttgc tggtgagaac
     2881 cagcggggag ctgcgcatct ccaattttct cctgtggcag atcgcctaca cggaaatctg
     2941 ggtcaccgac gtcctgtggc ccgatttcac cgagatggac ctgatcgccg gaattcgcgc
     3001 ctaccagggg cgcgaccgcc gctacggcct ccactccacg tagccggagc cgacccgtgc
     3061 acccgcaccc caaacgaagc gctgcggccc ccgcgcccgc caagccgcgc ggcacacgcc
     3121 gcaccgccat cgagcgcgtg gtcgcgggcg tggtcttcgt ccccctcttc atcatcatca
     3181 cgcgcgccgg cggctacgcc ttcctggccc tgatcgacct cgtgatcgtg ctcgggatgc
     3241 tggagttcta cgcgatgatg cgcgccaagg gcatgcaccc gtaccgcggc atcggcgtcg
     3301 cgtcgggcat cacgctctcg acctacatct tttttcgtag cggccaatac gcgaacttcg
     3361 tgttcacgtt cattctgatc gcgctgatgg gcctggagct cgcgcgcaag gacaaccgcc
     3421 gcgcggccta ccacgtctcg accaccatct tcggcgtgat ctacgtcgcc tacttgtcgt
     3481 cgcacttcgt gctcctgcgg gagctcccga acgcggtcgg agtcccgtac tcccaggggg
     3541 cgtcgttcgt gttcctggtc gtcgcggtga cgtgggcctc ggacaccggc gcctacacca
     3601 tcgggtcgct gttcggatcg cacaccctgc tcccgcgcgt gtcgcgccac aagacgtggg
     3661 agggtgcgct gggcggggtg ttgttcggcg cggtcgcggg ctggatctcg tcggtcagtt
     3721 tcgcgggcta cctgaaggct tgggaaggcg tggtcatcgg gatcctctcg tcgctggtcg
     3781 gcctgctcgg cgacctcttc gagtcgatgc tcaagcgcga cgccgatatc aaggacacct
     3841 cgaacctgat tcccggtcac ggaggtgtct tggaccgatt tgacagttta ctcttcaccg
     3901 cccctctcat atactacttc ctcaagttcg tgatttttca ataggttaca ccagttggac
     3961 gggctggatg cgccgtccta agccaacatg aagcgaatcg tcgtcctcgg gtcgaccggc
     4021 tcggtcggtc gcaacgtcct cgatgtggtc cgccgccacc cggaacagtt ccgggtggtc
     4081 gggctggccg cgcatcgcaa cgtcgacgtg ttgcgcgccc agtgcgccga ccaccccgac
     4141 gcgcgcttcg tcgtgaccga cgcggccgcg gccgccgaga tggccaatga ccccgcgcgt
     4201 cgcgcgggtc tcgctggtgt gggcgagccc gccctgctcg atctggtgcg cgggagcggc
     4261 gccgacctgg tggtcaacgc gctggtcggc ttcgtcgggc tggcccccac cctggccgca
     4321 ctcgaggccg gcatcccggt ggcgatcgcg aacaaggagt cgatcgtcgc cggcggcgag
     4381 ctcttgctgc gcgccgcgcg cgcgtcgggg gtcgagctgg tgcccatcga cagcgagcac
     4441 gtggcgatcg cgcagtgcct ggccggcgcc gcccgcgccg acgtcgagcg cgtcattctc
     4501 accgcgtcgg ggggggcgct ccgcgatcgc aaccccgcca gcttcgcaga ggtcacggtg
     4561 cgcgacgtgc tcgcgcaccc gacctggcgc atgggcgcga agatcacggt cgacagcgcg
     4621 accctggtca acaaggggct cgagatcatc gaagcccact ggctcttcgg gctgccgtac
     4681 gaaaagatcg acgtcgtgat ccacccgcag tcgatcgtgc attccgtggt gcgcttcgtc
     4741 gacggctcga tgatcgccca gatggcgact cccgacatgc gcatcccgat cctgtacgcg
     4801 ctcgcgtacc cgaaccgctt gaagtcagac ctaggcgccg acctgctcga gttcccggcg
     4861 ctgtccttcg cgcccgtcga tgcgcaccgc tacccgtgtt ttccgatcgc ggtcgcggcc
     4921 gcgaaggcgg gcgggaccgc acccacggtc ttgagcgccg tcaacgaagt cgcggtggag
     4981 gcattcctcg ccgggcggct ggcgtacacc gcgatacccg agtggatcgc atccgcgctc
     5041 gaagcgctcc ccgcgcggcc ggtcacttcc ctcgacgacg tggtcgcggc ggatggcgag
     5101 gcgcgccgtt ggattcgcga acaccatacg accgcgtccc gcggagacgg gcgctagaac
     5161 cgcgaggaat cgatgctcac caccatcttg gccggtctct tcgttcttgg cgtcgtcatc
     5221 atcgtgcacg agttcggcca cttcatcgtg gccaagctgg ccggcgtcta cgtcaaggtg
     5281 ttctcggtcg gcttcggcaa gcgcatactg cgctggcggc gcggcgaaac cgtgtacgcg
     5341 atctcggcgc tcccgttcgg gggctacgtc aagttcgcgg gcgagtcgga tctcaccgag
     5401 gtaccggcgc cggacccgga gacgccgccg ggaccgctcg acgagacccc cgactccgag
     5461 atcccgcgcg agcgctactt cacgacccag cgcaagctga tccgcgccgc cattctgctg
     5521 gccgggccgt tcatgaacta cttgctcgcg gtcgtcctct tcgtcgggat gtatttcgcg
     5581 cagggagtca gtgtggtgcc caccacgcgc gtggggcagg tgacggccga tgggccggcg
     5641 gcgctttctg gtatcgcgcc gggggacacc atcgcatcga tcgacggcgc gcgcgtggaa
     5701 gactggtacg acatcgagag cctgctcacc gacgacccct ccgagcgcaa ggccatcctc
     5761 gtgcgccgcg acgggcacga gcgcgagatc acgttcgagc ccaaggtcga gggtcggcac
     5821 gtctcgctgg ggatgttcgc cttcacgtcc tcgcgcgtgg gtcgcgtcca gaagaacgga
     5881 cccgcgtacc gggcgggact cgagaacggt gcggtaatcg aggccatcaa cgataccttg
     5941 gtgaccgagt acgcggacct ggttcgcatc atcaacgcga acgcggacaa cccgctctat
     6001 gttcgctgga cgatggacgg gctcgcgcac gcggatacca tcgtgccgga acccaagcag
     6061 gtgctcaagg ccggctccac ctcggagctg acgacggtcg ggatcatcgg catccacccg
     6121 tacgccgagc ggaggcgcga ggggttgttc acctccatcg gaaacggatt ctacgccgcc
     6181 aatgagacca ccgcgcagat cgtggggtac ctgggattgc tcttcaccgg gcggatgggg
     6241 gtcacgacgc tgggcggtcc catcaccatc acgcagatgg cgggcgacgt cgccaactgg
     6301 gggttcgact acttgctcgg attcctggcg ttcttcaaca tcaacctgtg cgtgttcaac
     6361 ctgatcccgc tgctgccgtt cgatggcggc cacttggcga tcctggccat cgaaggcgtc
     6421 atgagaaggc ccgtcaatcg gaggctgcgc gactggctga cgcagggcgg cttcgtgctc
     6481 atcatcttgc tgatggcgtt cgtcctggtc ctggacctgt cccgctgcgc gggatcaact
     6541 ccgagtggat tctgacggct gtgcgcgcgg gtctcccatg cgcgcttcga tcgcctggaa
     6601 aacgagggcc cagcgctcgg ggctcgcgtt gagcgcggcg gtggtgcgcg ccacacgcac
     6661 ggtgtcgatc tcgcccgcca tgcggtcgag gaggctgtct gcgaggacgc gctggtgggg
     6721 aaacatcgca ccggcgcgcc gaacgcggac gtaggattcg acgaggtaga cctcgtcggg
     6781 cgagagcccc gggtcggcgg gcttttcttt ggcgctgcac gcagcgaaaa ggacgagcac
     6841 cgcggcgacc acgagcggcc ggagctttac acgggggacg atgggtcgaa cgtcgcgcgc
     6901 agtcataggg agacgacagt ggtcgctcgt tagatacacc cgacggcgca gcggtgtcaa
     6961 ggcgtgggcg tgcgccggca ccatgg
//
LOCUS       DASPGK010000277         6109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_656496, whole genome shotgun sequence.
ACCESSION   DASPGK010000277 DASPGK010000000
VERSION     DASPGK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656496"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..949
                     /gene="aroE"
                     /locus_tag="VEC56_07540"
     CDS             80..949
                     /gene="aroE"
                     /locus_tag="VEC56_07540"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXV14043.1"
                     /translation="MIGHPVAHSLSPFIMNRAFDSLGLDAIYVAFGIKPERLAVSLAG
                     LSAMGVSGLNVTYPYKEEILYHLDAVSAEVEVISAVNTVVYLEDEIQGYNTDAVGTAY
                     ALEHFAGVQLEGARALVYGIGGSARAAAYGLLERGAERVVFAARSPENGPMVLERFQF
                     AFPEQSVGIITLDEAAAPARRDAFREANVVINATPVGMAGHAAGSLLEDPAWIRPEQV
                     FFDVVYHPKQTVFLETARRAGAKTLGGIALLVAQASESFRIWTDQGFDIKEMAEAVED
                     FSRTETSPGRRIN"
     gene            949..2091
                     /gene="aroC"
                     /locus_tag="VEC56_07545"
     CDS             949..2091
                     /gene="aroC"
                     /locus_tag="VEC56_07545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014926635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXV14044.1"
                     /translation="MAIRFLTAGDSHGEALVGIVEGLPAGLKLDERVIQRELARRRKS
                     YGRSSRQKIEKDEVSIVGGLWKGKTTGAPIAIVLPNRARTVKGKAGGGLETVPRPGHA
                     DFAGVMKYGFDEIPPVSERASARSTAMRVAIGAVAKAFLGHLGVDAIGHVRSIGSVDA
                     KSLRISPREIRKRAERSVVYCADGRAGRRMVTEIQRGKAEGNSLGGSVEVIVTGLFPG
                     IGSHVEWDRKLDARLAGAMMSIQSVKAVEIGDGLSTYRARGVDAHDAMRVERGRVLRP
                     TNHAGGIEGGMTNGEDVIVRLYAKPIPTAPRRASTFDLETLEPTESPYVRSDICVVPV
                     LAVIAEAVASWEILAAIMEKLGGDTVEETLAAKKRVVAALSKRLSQ"
     gene            2088..3731
                     /gene="aroB"
                     /locus_tag="VEC56_07550"
     CDS             2088..3731
                     /gene="aroB"
                     /locus_tag="VEC56_07550"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXV14045.1"
                     /translation="MSTSPVVILCGFMATGKTTVGRALAEMLGVPFIDTDELVEARAD
                     ASISEIFAREGEARFRDLEAEVCATLAGSPSGAVIATGGGALLRDETFRHLEKLGTMV
                     LLEASLVEVLRRASTMPARPKMERSFDAVRKLHDERKPTYHRVTWRVDTTHRRAEEAA
                     FEIAEQLRRAPDRGTVLHMRAGARPVPGRTARLGEDGLTRVVVGRGVLARLGDWMKDS
                     GLNGPVFVMVSRTVAGHYGTAAKAALDAAGLRNKFIELDDKEEAKTFEQAEGLLYELV
                     DAGATRDGVVVALGGGVTGDVAGFVAATYMRGLALVQVPTTLLAQVDSSIGGKVGVNH
                     PRAKNLLGAVHQPQLVLSDVDVLSTLPDREVSSGMAEVIKTAIIGSPELFARLEKAGK
                     KMPLRDGAFLEECVAACARVKARIVEEDPYEQGLRRVLNLGHTVGHALEAALGYGVLT
                     HGEAVAIGMLAALRLSVTRGLATASFETTTRKLIEACGLRTRVSEVGDDFLQAMNLDK
                     KRRASGLSFVLPVKPGEVRVVDDVSENEVVAAFQSTQKG"
     gene            3734..4231
                     /locus_tag="VEC56_07555"
     CDS             3734..4231
                     /locus_tag="VEC56_07555"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HXV14046.1"
                     /translation="MTILVIHGPNLNLLGTREPEIYGKKTLKEIDALIVAHGRKLGVE
                     IRTAQSNHEGVIIDLIQTAAGDGISAIVLNPGAYSHTSVAIADAIRSVKTPVVEVHLT
                     NVFARGGDRVKLVTATSAKGAILGFGFESYLLGVEAAVRVVGSSRSRKHTGLTVKRAR
                     SGRTR"
     gene            complement(4238..5302)
                     /locus_tag="VEC56_07560"
     CDS             complement(4238..5302)
                     /locus_tag="VEC56_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14047.1"
                     /translation="MSDDTLAVPLGGIVDAAVGPDGTIYLLDRQTCVVQRITSKGDVL
                     TPLGRKGEGPAELSQPALVVVDAMGRCTVVQDFYARAVCFDPSGQECAAADMSPLRDG
                     YFLTVYTRATVDHKGRLLVGTIRSKSGGASEGEGSASITRLGSPGEGGALRLLSSEEG
                     ATDAAMVRLPPKNRRLVYSGWDVSPRGVFAHADPGGTYSVYIGHTADGPRRKIVLPWK
                     GDDDHGVRRALEQSGIDTDPEAVAKVVSLAWLDDEYLLVQPVSEWSKLTPGVIGVYDL
                     VGKSGDVVGRWSVRCRYDPEADLTFIRRDRLIVIEGGASSLRAGLLASGMFVPSQGGG
                     TYPDEIRVSVYQLFADERMR"
BASE COUNT         1100 a         1986 c         2008 g         1015 t
ORIGIN      
        1 ccaagttttt cgcggacctg cgcacgctgg ttcgctagag gaagcgatgt cggcagaagc
       61 caggagtcat ttgttcggct tgatcggtca tccggtggcc cacagcctct cgccgttcat
      121 catgaaccgc gccttcgaca gcttgggact cgacgcgatc tacgtcgcgt tcgggatcaa
      181 gccggagcgc ctggccgtgt cgctcgcggg cctttcggcc atgggggttt cgggcctcaa
      241 cgtgacctac ccttataagg aggagatcct gtaccacctg gacgcggtct cggccgaggt
      301 ggaggtcata agcgccgtga acaccgtggt ctatctcgaa gacgagatcc aggggtacaa
      361 taccgacgcg gtcggaaccg cgtatgccct cgagcacttc gcgggcgtgc agctggaggg
      421 cgctcgcgcc ctcgtgtacg gaatcggcgg gtcggcgcgc gcggccgcgt acggattgct
      481 ggagcgcggg gccgagcgcg tcgtgttcgc cgcgcgatca ccggagaatg gaccgatggt
      541 gctcgagcgg ttccaatttg cgttccccga gcagtcggtg ggcatcatca ccctcgacga
      601 agcggccgcg cccgcgcgcc gcgacgcgtt tcgcgaagcg aacgtcgtga tcaacgcgac
      661 gccggtcgga atggcgggcc acgcggccgg ttcgcttttg gaagatccgg cgtggattcg
      721 acccgaacaa gtgttctttg acgtcgtgta ccaccccaag cagaccgtgt tcctcgagac
      781 cgcgcgccgc gcgggtgcca agacgctggg cggtatcgcg cttctggtcg cacaggcgag
      841 cgagagcttt cgcatttgga ccgaccaagg cttcgacatc aaggagatgg cggaagcggt
      901 ggaggatttc tcgaggacgg agacgtcgcc cgggcggcgg atcaactgat ggcgattcgt
      961 tttctgaccg cgggcgattc gcacggcgag gcgctggtcg gcatcgtcga aggactgccg
     1021 gccggcttga agctcgacga gcgcgtgatt caacgtgagt tggcgcgtcg tcgaaagtcc
     1081 tatgggcgca gcagccgcca gaagatcgag aaggacgagg tgtcgatcgt gggcgggttg
     1141 tggaagggca agaccacggg cgcgcccatc gcgatcgtgc tccccaaccg cgcgcgcacc
     1201 gtcaagggca aggccggcgg cggcctcgag accgtgccgc gtcccggcca cgccgacttc
     1261 gccggcgtca tgaagtacgg cttcgacgag atcccccccg tgagcgagcg cgcgagcgcg
     1321 cgcagcaccg cgatgcgcgt cgccatcggc gcggtcgcga aggcgttcct cggtcacctg
     1381 ggtgtcgacg cgatcgggca cgtccggtcg atcgggagcg tggacgccaa gtcgttgcgg
     1441 atttcgccgc gcgagatccg caagcgcgcg gagcgctcgg tcgtgtattg cgccgatggg
     1501 cgcgcgggcc gacgcatggt caccgagatt cagcgcggta aggcggaggg caactcgctc
     1561 ggcggctcgg tggaagtgat cgtcaccggc ctcttccccg gtattggcag ccacgtcgaa
     1621 tgggaccgca agctcgacgc gcgcctggcc ggcgccatga tgagcatcca aagtgtgaag
     1681 gcggtcgaaa tcggcgacgg gctttcgacc taccgcgcgc gcggtgtcga cgcccacgac
     1741 gcgatgcgcg tcgagcgcgg gcgcgtgctt cggcccacca atcatgcggg cggcatcgag
     1801 ggcggaatga ccaacggtga agatgtgatc gtgcgtctgt acgcaaaacc catccccacc
     1861 gcacctcggc gcgcgtccac tttcgacctc gagacactcg aacccaccga gagcccgtac
     1921 gtgcgctccg acatctgcgt ggtgccggtg ctggcggtga tcgcggaagc cgtggcctcg
     1981 tgggagatcc tcgcggccat catggagaag ttgggcggcg acaccgtcga ggagacgctc
     2041 gccgccaaga agcgcgtggt cgccgccctc tcgaagcgcc tatcccaatg agcacgtccc
     2101 ccgtcgtcat tctgtgcggc ttcatggcca ccggcaagac caccgtgggc cgcgcgctgg
     2161 ccgagatgct gggcgtcccc ttcatcgaca ccgacgagct ggtcgaagcg cgcgccgacg
     2221 cttccatctc cgagatcttc gcgcgcgagg gcgaggcgcg atttcgcgac ctcgaagccg
     2281 aagtgtgcgc gacgctcgcg ggcagcccga gcggggccgt gatcgcgacc ggcggcggcg
     2341 cgctcttgcg agacgaaacc ttccggcatc tggagaagct cggcaccatg gtgctgctcg
     2401 aggcgtcgct cgtcgaagtg ctgcgccgcg cgtcgaccat gcccgcgcgt cccaaaatgg
     2461 agcgctcgtt cgacgccgtg cggaagctcc acgacgagcg caagcccacg tatcatcgtg
     2521 tgacctggcg cgtcgacacg acacaccggc gcgcggagga agccgcgttc gagatcgccg
     2581 agcaactgcg acgcgccccc gatcgcggga ccgttctcca catgcgggcc ggcgcgcgtc
     2641 ccgttcccgg gcgcacagct cgtttgggtg aggacggact cacgcgcgtc gtcgtcgggc
     2701 gcggcgtgct ggcacggctc ggcgactgga tgaaggactc cgggttgaac ggacccgtgt
     2761 tcgtaatggt gtcgcgcacc gtggccggcc actacggcac cgcggccaaa gcggcactcg
     2821 acgcggccgg ccttcgcaac aagttcatcg agctcgacga caaggaagaa gcgaagacct
     2881 tcgagcaggc ggaaggattg ctgtacgaat tggtcgacgc cggagcgaca cgcgacggcg
     2941 tcgtcgtggc cctgggcggc ggcgtcaccg gcgacgtggc cggcttcgtc gcagccacgt
     3001 acatgcgcgg gctggcgttg gtgcaggtgc ccaccacgct gctcgcgcaa gtcgactcca
     3061 gcatcggcgg gaaggtgggc gtcaaccacc cgcgcgcgaa gaatctcttg ggggcggttc
     3121 atcagcccca actcgtgctc tccgacgtcg acgtgttatc gacgctcccc gaccgcgagg
     3181 tctcgagcgg catggcggaa gtgatcaaga ccgccatcat cgggtcgccc gagttgttcg
     3241 cgcgcctcga gaaggccggc aagaagatgc cgcttcgcga cggcgcgttt ctcgaggagt
     3301 gtgtggccgc gtgcgcgcgc gtcaaggcgc gtatcgtcga agaggatccc tacgagcagg
     3361 gcctgcgccg cgtgctgaac ctcgggcaca ccgtcgggca cgcgctcgaa gccgcactcg
     3421 ggtacggcgt actgacccac ggcgaggccg ttgccattgg gatgctggcg gcgttgcgac
     3481 tctccgtcac gcgcgggctc gcgaccgctt cgttcgaaac cacgacgcgc aagctgatcg
     3541 aggcgtgcgg cctgcgcacc cgggtcagcg aggtcgggga cgatttcctg caggccatga
     3601 acctcgataa gaagcgccgc gccagtggac tctcgttcgt gctgcccgtc aagcccggcg
     3661 aggtgcgcgt cgtggacgac gtctccgaga acgaggtcgt cgccgcgttc cagtcgaccc
     3721 agaaaggatg accatgacga tcctagtcat tcacggtccc aacttgaatc tgcttggaac
     3781 gcgcgagccc gagatctacg gcaagaagac cctcaaagag atcgacgccc taatcgtggc
     3841 gcacggccgc aagctcggcg tagagatccg gaccgcgcag agcaaccacg agggcgtcat
     3901 catcgacctc atccagaccg ccgccggcga cggaatctcc gccatcgtct tgaatcccgg
     3961 ggcgtactcc cacaccagcg tcgccatcgc ggacgcgatc cgctccgtga agacgcccgt
     4021 ggtcgaagtc catctgacca acgtgttcgc gcgcgggggc gaccgcgtaa aactggtcac
     4081 ggccaccagc gcgaaaggag ccatcctcgg cttcggcttc gagagctacc tcctgggtgt
     4141 cgaggcggcg gtgcgggttg tcggctcctc gcgaagtcgc aagcacacgg ggcttacggt
     4201 gaaacgagcg cggagcggca ggactcgata ggtcgcttca ccgcatccgc tcgtcggcga
     4261 acaactggta gaccgacact ctgatttcgt ccggatacgt gccgcccccc tggctgggga
     4321 cgaacattcc cgaggccagg aggcccgctc tgagcgatga tgccccaccc tcgatgacga
     4381 tcaggcggtc gcgccgtatg aacgtcaagt cggcttcggg atcgtaccga caacgaacgg
     4441 accatcgtcc gacaacgtcg ccgctcttgc cgacaaggtc gtacactccg atgacgccag
     4501 gcgttagctt tgaccactcc gagaccggct gaacgagcag gtactcatca tccaaccacg
     4561 caagagacac gaccttggcc accgcttcgg ggtcggtgtc gatgccactc tgctccagtg
     4621 cgcggcggac cccgtgatcg tcgtcccctt tccacggcag cacgatcttc cgacgtggac
     4681 cgtcggcggt gtggccaatg tagaccgagt aagtgccgcc ggggtcggcg tgtgcgaaaa
     4741 cgccgcgcgg actaacatcc cagccggagt aaacgagtcg cctgttcttc ggaggcaaac
     4801 ggaccatcgc tgcatcggtg gcaccctcct cggacgaaag caatcggagc gcgccgcctt
     4861 cgcccggcga accgagtctg gtgatactcg cgctaccctc gccttccgac gcccccccgg
     4921 atttgctccg aatcgtgccc acgagcaacc gacctttgtg gtccaccgtt gcccgggtat
     4981 acacggtcag gaagtagccg tcgcgcaacg gactcatgtc cgcggctgca cattcctgtc
     5041 cggacggatc aaagcacacc gcccttgcgt agaagtcctg aaccacggta cagcgcccca
     5101 tcgcgtcgac cacgaccagt gcgggttggg acagttccgc cgggccctca cccttccgac
     5161 ccagaggagt aaggacatca cccttcgacg tgatgcgctg gaccacacaa gtctgtcggt
     5221 cgagcagata gattgtgccg tcaggtccga ccgcggcatc gactatgccg cccaacggaa
     5281 ccgcaagggt atcgtccgac accgaacaga gctcccttgg gtcatggcgc cgaggtttcg
     5341 cacggtcggc gatcgactcg atcggtccat tgagaagcaa cagagccgac acgacagcaa
     5401 gttgtttgag gggcggagta ttacccatcg aactggcagt tcaagtctac ttgatgagcc
     5461 gcattcttaa gcaactgata gaggggctgg cagcacacgt cgcagagcac gctcgtcgta
     5521 ctcatgcact ggttgtactg caccaacgcg ccataaaatg caagtgtcgc cgctgtgcac
     5581 gagtcaatga tcggcaccgc cgtagtctgg acgtcaccga cgaggacaac gaggcaaagc
     5641 gcacagagca cgaacaggat cttcctcatc acgtctcctc cattccactc catggtatgc
     5701 gactaggtcc tttccttcat cttgtcgcgg cgtcaggtca cgtttctcgt tgacgtcgcg
     5761 gacggtgcca gtcgcgtgca aataccgatc ctgtccctgg caacgcagcg taacctgaag
     5821 gcacgccgaa gagtccagcg ttgcagctgc gccgacgctg ccgacggcac tccgcgttcc
     5881 gagcgcgttc gtttcgctgg gaggggcatc tccaggcgtc accatgcgac aaatcgctcg
     5941 tcaagctggt tcgcgtccgc tgtcgaagaa tcgacaacgg actgtcgctc cgacgtagta
     6001 ggcgaccgct cgacgttgat gggcggaacc gagaataccg ccactaggcc tacgacttcg
     6061 cgggaatatt gaggcgtttg agcttcgcga tgagcgtcgt gcggggaac
//
LOCUS       DASPGK010000278        26484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1124921, whole genome shotgun sequence.
ACCESSION   DASPGK010000278 DASPGK010000000
VERSION     DASPGK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 26484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26484
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124921"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VEC56_07565"
     CDS             complement(<1..1184)
                     /locus_tag="VEC56_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14048.1"
                     /translation="MDTKRLIVAARLAPLVLCGLAGAGVMPARAAAGACGDGLVCVSP
                     ARVTFAITPSGAWKFFGPANERSFLDKGGVLLYARAQSGVETELLNATGGVKSLLSTP
                     IDPVKEGARGGARDPFPRPDDDYDGKIDEDPLDRADNDGDGRVDEDFAAIGDEMSVAV
                     YGPKGSSGIEIRQELYAWSLPHIDAMAATAISVRAGKTALDDLRVGIFVQPAGNFESE
                     PAPLIESDRLGASTSAGETLVWRDGGNGVALLVWAPSAGAKDWEVREDRKGTRVLSPL
                     LGQLAAGAEITIYAALIALPPDDLRSARAIQAAWRTLAGKKGARLIPPPVSLTVKPEE
                     TAGESEVEESAVGSPYGAITSAQLFWSTPGKLAPSLLVGSPNPFRDAIAIDYEVPETV
                     VD"
     gene            complement(1190..1819)
                     /locus_tag="VEC56_07570"
     CDS             complement(1190..1819)
                     /locus_tag="VEC56_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HXV14049.1"
                     /translation="MRLKMEKISEFTVRRLSNYYRILLDLEKRGIPTVSSARLAELGG
                     ITSAQVRKDLSYFGNFGTRGLGYSVTELKGAIVEILGLDKQWTMALFGAGSLGHALFF
                     HEGFRDDGFFFKHVFDVDPAKVGTKWNDVEITHQRHAEDVLRRDPVHIAVVATPPEAA
                     ADIVDLLVRSGTKGILNFAPTQLAVPEGVWLRNVNLAVALESLSFYLSM"
     gene            complement(1820..4258)
                     /gene="gyrA"
                     /locus_tag="VEC56_07575"
     CDS             complement(1820..4258)
                     /gene="gyrA"
                     /locus_tag="VEC56_07575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXV14050.1"
                     /translation="MANNERIVPVPIESEMRNSYLDYSMSVIISRALPDVRDGLKPVH
                     RRVLTAMNDLSLTHTRAYRKSAKVTGDVTGNYHPHGTTAVYDTIVRMAQEFALRYPLV
                     DGQGNFGSVDGDPAAAERYTEVRMTAFAEEILADLDKETVDYGPNYDESRTMPLVMPS
                     RVPNLLVNGAAGIAVGMATNIPPHNIVEICDASIAVLHNPEVADDDLLKIVKGPDFPT
                     AGIVLGKLGISEAYRTGRGRVIVRARTHVEEMKGDREAIIIDELPYQVNKAQLIERIA
                     ELVKDDVISDIADIRDESSREGMRVVIILKRDADVRVVENQLFKHTQMQVTFGIIMLA
                     LVNNRPEVLSLHDMIQHWLNHREAVVVRRTKFDLRKAEERAHILEGLRIALDHIDEVV
                     ALIRASKDVETARSGLMTKFKLSEIQANAILDMRLQRLTGLERQKVEDEYQELLKLIA
                     ELKSILADRKKVLSIIETEILEIKEKYGDERKTEILDSGPVDFNVEDLIAEEDMVITI
                     SHAGYIKRLPLDAYRQQKRGGRGVTGIKMRDEDFVEHIFVASTHSYILFFTDMGRCHW
                     VKVHEIPEAGRAARGKAIVNLLGLQEGERIAGRIPVSQFREDQFLVLATKKGVIKKTV
                     LSDYGRPRRGGIIAVNLRDGDELIDAAITNGGQDIVLAKKNGRSIRFHESGVRAMGRA
                     ATGVKGTTLVGDDEVVGMVVVAAGATLLTVTENGFGKRSPLEDYPVKNRGGIGVINIK
                     TTARNGKVVTIQEVKDNDQMMIITKNGIVIRCPISGISVIGRNTQGVRLINLDGDDKV
                     VDIAHLAMEEEAEA"
     gene            complement(4449..4736)
                     /locus_tag="VEC56_07580"
     CDS             complement(4449..4736)
                     /locus_tag="VEC56_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HXV14051.1"
                     /translation="MDRRVKQTWKLTYRTARRIVIAVIGVTVVLLGIAMLVLPGPGLL
                     TIVGGLAILGLEFAFAKRWLARIKQTARWAVDEAKRRVGRKSPPDGPPPTP"
     gene            4836..7499
                     /locus_tag="VEC56_07585"
     CDS             4836..7499
                     /locus_tag="VEC56_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV14052.1"
                     /translation="MELKRLAHYEIVGPIGAGGMGEVYRARDTKLGRDVALKLLPEHF
                     ARDADRLARFEREARLLASLNHPRIAAIYGLEQDGAHRFLVLELIEGEDLSKRIARGP
                     VPLDDALGIALHVCEALEAAHETGVVHRDLKPSNIVVTPNGSVKVLDFGLAKALDPAS
                     GNSNLTQSPTILGSSPTMQGVILGTAAYMSPEQARGRAVDKRADIFAFGCVLFEMLTG
                     KLAFSGETVSDTLAAVLRADPDWGALPKDTPAAIARLLRRCLEKDPKRRLRDIGEAAL
                     VIDDVRSGRIVDEPTAVAAPSIPKARFVGPRAIAVVAIVLAAVAAGSSALTRLLSPSE
                     AEPPFRKLLLDVVLTDSLGAPEAPRISPDGKRLAFHSGGRLWIRDLDNLEPRAIAESS
                     SEVAPFWSPDGAFVGYVTSQRLWKVRAAGGDPTPVCDSPASFGGGAGASWGDDGRIVF
                     SYGNGGVYEVSSSGGDAREIIPVDVSVEQDIHQPAMLPGGKGILYIQHRQQSGPDTIQ
                     LFANGTRKTLLTLTGQNLWSVEYAPSGHILFVRIPDNAGLWAAPFSLQSLEITGEPFL
                     IAPKSSEGSASNDGALAYLPTLFGDMVRVARYDGAGKFVSYATETLTNVNSIALSPDG
                     SKLAVSMRDAATRDVWVIDLVRNTRSRLTFAGNYNTQPAWSPDGKTIAYRNVDDQSLY
                     LIPADGTGDRRFLTRGASFPCYSPDGRWIVFSTRLSGSAGNQDSDLFMVDASLTDTTR
                     VTVFAGTGDQRYPVVSPDGNYVAYLSNESGNDVVYLTTFPRSTGKWQVSVHGDANWPS
                     WAPTGDRIYFVADDRVEMVTFEPGAAPRLGTPQKMLDTTRARLELWGFQKLDVDIDGR
                     GILVVEATRSVEQQDGVVFVENWLEEFSQDR"
     gene            complement(7515..8606)
                     /locus_tag="VEC56_07590"
     CDS             complement(7515..8606)
                     /locus_tag="VEC56_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV14053.1"
                     /translation="MTPWPAQSSGSDYLTWTKRRVPVRYNLARSGVPRLALERLELTL
                     SDLLHNQNAEDGWPPLMERIAARQELTPAHVVTTHGCSMANHLAMAAILEAGDDVVME
                     TPVYEPLVRVAEYLGARVIWFARHAENRWHIDPEEVRRALTPKTKLVVVSNLHNPTGA
                     FDNQDTLSDVARAAESVGARVLVDEVYLEFFHARGARSAVGQSPRILATSSMTKVFGL
                     DGLRLGWVLAEPELAERMRRLNDLFSVNTAHPSERIAARALEHADALLAESNALLARN
                     IELVDAFVRSHDDLSWAKPRAGTVGFVQVADRDVDRLVDRLHAEFEVAVVPGRFFGAP
                     DHFRISFGLESDLMREALSRIGRVLRERG"
     gene            complement(8632..10533)
                     /gene="gyrB"
                     /locus_tag="VEC56_07595"
     CDS             complement(8632..10533)
                     /gene="gyrB"
                     /locus_tag="VEC56_07595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXV14054.1"
                     /translation="MTHEYTAKQIQVLKGLEAVRKRPAMYIGSTDIHGVHHLIYEVVD
                     NSVDEALAGHCSDIEVVIHEDLHVSVEDNGRGIPVDMHPTQHRPAVEVVMTTLHAGGK
                     FDRDSYKVSGGLHGVGVSVVNGLSEWLDVEIHRDGTRYTQRYERGIPASDLKELGTTD
                     RRGTIVSFMPDSEIFEKVDYQYETLAQRLRELAFLTKGLKIVFDDRRAGKPRRQVFQY
                     EGGIVSFVKYIDTAKTVLTPQPIFIQAEKDGVQVEVALQYTDGYAENVFSFANNINTI
                     EGGTHLVGFKAALTRTINNYASSANLFKKERVTLGGDDVREGLTAVVSVKLAEPQFEG
                     QTKTKLGNSEVKGIVEAVVGDGLREYLEENPSIARSVVEKTLAAFSAREAARKARDLA
                     RRKTALESAALPGKLADCAARDPAVSELFLVEGDSAGGSAKMARNREYQAILPLRGKV
                     LNVEKARLDKIFSNEEIRTIITAVGTGGGEFDLTKVRYHKIIIMTDADVDGAHIRTLL
                     LTLFYRHMKPLVEAGYVYIAQPPLYKLKKGKKEVYAYADEDRDRMLKDWGGNDGVYIQ
                     RYKGLGEMNPEQLWETTMDPEARTILKVDVQDAVEADRIFSILMGDEVEPRREFIQEN
                     ALSVKNLDV"
     gene            complement(10637..10942)
                     /locus_tag="VEC56_07600"
     CDS             complement(10637..10942)
                     /locus_tag="VEC56_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXV14055.1"
                     /translation="MTRRSFARIGDLLPSVLRDLGLQKRFSERQLVERWADIVGPEIS
                     QRARATRYENGTLYVRVDHGAWMQELHFIEKELVKKLRAACPDVNLTRIRLGARDME"
     gene            complement(11063..12160)
                     /gene="recF"
                     /locus_tag="VEC56_07605"
     CDS             complement(11063..12160)
                     /gene="recF"
                     /locus_tag="VEC56_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXV14056.1"
                     /translation="MRVDRFASTNFRNLLEGEIPFGPGLNVLVGDNGQGKTNLLEAVY
                     FFRFGRSFRAQVDTELIRFDAPFCRVELACTFADGRREDFAFSIERREGRAAKAIKVN
                     GELLPRRGDLAGRFPAVLFGPHDLRIVSGEPENRRRFLDTIGIMTDPGYLRTATKYRR
                     VVEQRNAALKARASRDEMAAWNDRLIELGADFILRRRALTTAMEREMRAQASDLQSPF
                     DFVMRYDSDLLADTEDAASLTRAFQDTLASIASEEYRRGVTLAGPHRDDVELVMDGHD
                     LRRFGSQGQRRLLAVLLKLAETSYLENELREPCVLLLDDVFSEFDEAIIAKLHRFLDG
                     SRQVFVTSPANLASDARARVHRVWAGRISSA"
     gene            complement(12174..13286)
                     /gene="dnaN"
                     /locus_tag="VEC56_07610"
     CDS             complement(12174..13286)
                     /gene="dnaN"
                     /locus_tag="VEC56_07610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXV14057.1"
                     /translation="MKITVDQKDLLKGLQGIAAVVPSKTSLPILSTFLLETRGDGKIT
                     LTANDLDVSLTQTLACETSGPGSVAVPGKKFFEIARSLPDDKVVIEVEGDRISIKCRR
                     SRFKMTGKSADEFPKLPSQKAAVTFPIAASVLDRMINKTSYAVSTDLTRPSLCGVLWE
                     IKKGALGMIATDGHRLAKVQLPGAVGGGEEKDVIVSPKALNIYRSLAADMDSVHISLA
                     ENHITFDLGDSVIYSRLLEGPFPDYNQVMPKKNTRKLTVSRDELTDACRRVSILSSAI
                     THQVKLVIEDDRLTLSVKTPDVGEALEEVTCSYTSPKMEIGYNARYLLDILRTVESPD
                     VSFLLDRNDNAGVVTPEQDGEEIKHECLLMPLRLSD"
     gene            complement(13862..15217)
                     /gene="dnaA"
                     /locus_tag="VEC56_07615"
     CDS             complement(13862..15217)
                     /gene="dnaA"
                     /locus_tag="VEC56_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXV14058.1"
                     /translation="MSVSSRDHMWSEFLATVATLVNKQTFETWFKPMRLNAVDGSKVV
                     LDCPNKFFVDWVSEHHSEKIAFALRQVLGTDPQISLHNANDAPPDISPRRAELVPAPM
                     APRARKPALEGERLNPKYVFDEFVVGSNSRMTYAACMAVSERPAQVYNPLFIYGGVGL
                     GKTHLMQAIGHVVLKSHPTARVHYSSAESFMNEMIMAIRQANGHEFRTKYRNVDLLLI
                     DDVQFLAGKESTQEEFFHTFNALHGANRQIVVTSDRPPKEIPTLEDRLVSRFEWGLIT
                     DIQPPDYETRLAILRRKVERENISIPDDVLALIADSVKSNIRELEGSLVKLLVHASVY
                     KQEITGEIARDVLKDFIKSTPKKTSITLIQKVVSQHYRVPVESMRSKVRTARLAFPRQ
                     VAIFLARDMTSASLAQIGQRFGGRDHTTVIHACQKISDMIERDVSLRSTIGQLRKELS
                     S"
     gene            15815..15949
                     /gene="rpmH"
                     /locus_tag="VEC56_07620"
     CDS             15815..15949
                     /gene="rpmH"
                     /locus_tag="VEC56_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXV14059.1"
                     /translation="MKRTFQPRNLKRKREHGFRKRMSTRGGRLVLKRRRAKGRKRLAA
                     "
     gene            16038..16376
                     /gene="rnpA"
                     /locus_tag="VEC56_07625"
     CDS             16038..16376
                     /gene="rnpA"
                     /locus_tag="VEC56_07625"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXV14060.1"
                     /translation="MRTLTHAWQFRRCYDSGKKLVARHAVIFLHPSEADDGLRVGVVA
                     SRKVGGAVRRNRAKRLLRETARRIAPKWIDRHIWVVLVAKASIVDARADEIEDDIGRA
                     LIAGGELDIE"
     gene            16421..16690
                     /gene="yidD"
                     /locus_tag="VEC56_07630"
     CDS             16421..16690
                     /gene="yidD"
                     /locus_tag="VEC56_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXV14061.1"
                     /translation="MLRASLLALIAGYRRLVSPLLPPACRFHPSCSEYASEAIARHGV
                     VRGGVLAAGRLARCHPWCEGGIDPVPPARGSREANARAPRVEGVR"
     gene            16687..18534
                     /gene="yidC"
                     /locus_tag="VEC56_07635"
     CDS             16687..18534
                     /gene="yidC"
                     /locus_tag="VEC56_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXV14062.1"
                     /translation="MSKTFGMERRALLAFAASMALFLLYDALYLAPKMREERARREAV
                     QLEEAQKIARLQKVDASRDTLSVGGDQPALVPAPAGDSLSMAFAPADTGIAADEPSQR
                     IVVASPLFEITLDTRGASIEGVHLLRFAGANGPVELLPPGDSWSGDRMLSATIRGSAR
                     ELAVEKIVFEASAGRQALSDGARVNVAAGDSVDVVFRAAGPGGMIERAYRFYGDRYDF
                     RTTLRASEALVPGAETVAWSFGPGLTATEKNARDDQSKFRASVLLGQEFHRKHPGDFG
                     RANVEKFSGTLNWASLQTKYFLAAVIPPQPVRAEIEMSGIKSEHRVSQSVAIPATVAR
                     GEITSELRGFFGPLDFKLVEGLGVGLEKNVEFGWKFIRPVSVAVLWSMNALHKVIPNY
                     GWVIVIISILTKVLFYRLTHKSFKSMKEMQDLQPRIAALKEKFGSDRRRLSEETMKLY
                     KEAGVNPLGGCLPMLLQMPVFIALFNVLSHAIELRGAPWIAWINDLSQQDVLFKLPAS
                     LPLVGDAFSLLPLLMGASMWAQSKLGGSPTGQPGNPMPPGFNTILPIVFTFLFYKMPS
                     GLVIYWIINTALSVAQQYYIVKSHKAPALAVVPESAAPSGKKRRARKSS"
     gene            18545..19222
                     /gene="jag"
                     /locus_tag="VEC56_07640"
     CDS             18545..19222
                     /gene="jag"
                     /locus_tag="VEC56_07640"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HXV14063.1"
                     /translation="MAHSVETTGKTIDQALEKALKELGARKDEVDIEVLSETHGGLLG
                     IFGGKMVRIRVTSKNAKAPAGAAEGADVVRSVIGDVLGFMGVEYTLSIEESDDTTFVN
                     INSSGLDGLLIGRRGETLASIQHVVNRIFTKRTGRHSKITVDVGGYVRRKHRLLVEKA
                     QKLAERVSKTQKEYDFEPLKASERRIIHLAVADFDDVTTYTIGDGLLRKVVISPKTSA
                     TDDLPRD"
     gene            19333..21585
                     /locus_tag="VEC56_07645"
     CDS             19333..21585
                     /locus_tag="VEC56_07645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXV14064.1"
                     /translation="MPIVALLAMLLPYAVEAASLLGVVRARDTGAPIGFVNVQVSGRS
                     QAGAVIQGGVLAGENGSYLIEPLPLGRYRVAFTRVGYRAREDSVIVAEERTYVLDIAL
                     ELEPVQIEGVRVEADTDREFTETEDVQTGFVSIRADRLSSLPGIAEGDPVRALDLFPG
                     VQSASDFSSALYVRGGGPDQTLVLLDQATVYNPTHAFGFFSTFNADATGDVNLYKGAY
                     PAEHGGRLGAVLDVRSREARAQEFLGKVGISTIASRVALDGAVRSGSWSVSARRTHLE
                     PMLDAIRDEDTEVPDYFFYDLNGKISLPTGGGRFDVSVYHGRDDLDFFPGGDTSLELR
                     WGNTVATAAFMRALSDRLIVSARASFSDYESNTVASAFATPATFENAVQDITVGGDVK
                     WEASLRHTFTAGLQASTYDVAFSQTFNGQLVDDYRREPVEVAAFLEDRWTPWGDTAFD
                     LGMRARYIDDGERYLFEPRVAFAQVFRPDLRVKVGAGIYHQYLQLVATEAVAAVDFYV
                     PIDETAPLGRSWQLVAGADWRWKPSVLFSIEGYYTDLENLVLLDNTSTVNVEVETADD
                     LFYLGGDGYAAGVELFARRDMGSVTGWIGYTLGWTRRAFPEIEDGETFAPKYDRRHDI
                     SAVAEHRRGPWRFTGSFVFATGQAFTPVSGRYSLRDPTTGVVPSDIQLLYADKNSARL
                     LAYNRLDLGVARDFSFFGAKAEWLIEVFNVYSRRNEWFVEYQRDEEVVDAEVIRMLPI
                     IPSIGINVWF"
     gene            21576..22514
                     /locus_tag="VEC56_07650"
     CDS             21576..22514
                     /locus_tag="VEC56_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HXV14065.1"
                     /translation="MVLKRARILAVGIAVWALSCSPEREGSELFAPGGLGIPVVDAVL
                     VVGQPFSDVYLSQTLAPDERFTRERAGLNGATVTIEDGARVIPYTSMGVRGWYRSGAP
                     DIVQASTTYRFTATLTDGRVVRATTTTPARLSVREWVLLDESGTNVLQTLATFEEHGD
                     TVYFEPQNQLVYPENILAAVLDDQSTAGYQIGLINLETDSPLLIDADFLEPDDLEDFK
                     RTNSSPPLNYESTLRIPWLAIYYEGRYNLRVVALDRNWYDIARTDPAIGGGGFGFGGE
                     AGDTSKRPIFHVEGGIGVFGSVSSDSVGFYVNPPAQ"
     gene            22630..24018
                     /gene="mnmE"
                     /locus_tag="VEC56_07655"
     CDS             22630..24018
                     /gene="mnmE"
                     /locus_tag="VEC56_07655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXV14066.1"
                     /translation="MHSTTDTIAALSTAPGEAGIAVIRVSGPEALGVLDRVLRTPADR
                     RFEGDWEHRRLYHGGFFDPAGERVDDVMAAVMRAPDSYTGDDVVEISCHGGSAVVARI
                     LETLFASGARPAQPGEFTQRAFLNGKMDLIQAEAVADMIHARTELQRVVAERQLRGAL
                     SRRIDALADDMMTLLAVVEANIDFIEEGIDALDVPAALATIARQRPELDDLLSTSSLS
                     RPLREGYRVVIAGPVNAGKSSLFNRLTGEPHAIVTEIPGTTRDVLRETVVIEGVPFIL
                     HDTAGLRDGTPDRVEAIGIGRAVDAAESADVVVFVLDASERALAGDVREALARLDASR
                     TLVVWNKVDRGEDVDAGAAPRDIAHVRASAVTGQGVDEIRRELLRLAGSEVLARTARD
                     RAILNARLVTLLADARARLDTLGQAVESREPLELLAVQARDVLSLYEEATGRRYHDGL
                     LDVIFSRFCIGK"
     gene            24092..25957
                     /gene="mnmG"
                     /locus_tag="VEC56_07660"
     CDS             24092..25957
                     /gene="mnmG"
                     /locus_tag="VEC56_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXV14067.1"
                     /translation="MDRRFDIIVIGGGHAGCEAALAAARMGMHALLSTLRRDRIGHMP
                     CNPAVGGLAKGHLVKELDALGGEIGRATDRAGIQFRMLNRAKGPAVWSPRAQVDKVIY
                     SRIMRETLEAQPGLTIVEGEIVDLIVNQGAFVGVRFASGEIVNAGACVLTTGTFLRGL
                     MHTGDRRTDGGREGEPPAATLSESLRSLGLRMGRLKTGTPPRVFRDSVDLSRIDPQPG
                     DDPPAPFSYRTARIELPQVPCHLTYTSEATHEVIRRNLHRSPLYGGAIEGIGPRYCPS
                     IEDKVVRFADKDRHQVFIEPEGLDHPELYLNGISTSLPVDVQLEILSSIPGLESARMA
                     RPGYAVEYDFFPPDQLHRTMESKCIERLYFAGQVNGTSGYEEAAAQGFLAGVNAVLKI
                     QGEAPFVPRRNEAYLGVLVDDLTTKEIDEPYRMFTSRAEFRLVLRQDNADERLMATGH
                     RFGLVPERALATVRERVRRVDEVVDALGRRGFPAREGAARFDEWGLEPVRHGTTLRDV
                     LRRPGVRLAHLRDLAGLDCAPSLDDAVECRVKYEGYIDRQARDVQALSDLEARRIPPD
                     FAFEALAGLSTEAREKLRIKRPETLGQASRIAGVRASDLSILAVHVERARRTHAG"
     gene            25954..>26484
                     /locus_tag="VEC56_07665"
     CDS             25954..>26484
                     /locus_tag="VEC56_07665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HXV14068.1"
                     /translation="MSVPENDLETTLADVVRLFPALSIDPLRRYTREILRWNDEIGLV
                     SRKHPVAACHRLLIESAEFGGVVAGVLAPDRSTDLRVADVGSGAGFPGFVWRMLFPGW
                     DLALIERREKKAVFLEQIVRQFSFSRVEVFAGDARAASHLERYRASFDVVATMAVGAP
                     SRTAPEIEDLLVAGGVF"
BASE COUNT         4430 a         8545 c         8662 g         4847 t
ORIGIN      
        1 tcgtcgacca cggtttccgg gacttcgtag tcgatggcga tggcgtcgcg gaacggattg
       61 ggcgagccga ccaggagcga gggcgccaac ttgccgggcg tgctccagaa cagctgcgcg
      121 ctggtgatcg cgccgtacgg agaaccgacg gccgattcct cgacttcgga ctcgccggcg
      181 gtctcttccg gcttcaccgt cagcgacacc ggaggcggaa tcaagcgcgc gcccttcttg
      241 ccggcgagcg tgcgccacgc cgcttgaatg gcgcgcgcgg agcgaagatc gtcgggggga
      301 agcgcgatca gcgcggcgta gatcgtgatt tccgcgccgg cggcgagttg accgagcaac
      361 ggcgagagca cgcgcgtgcc cttgcggtcc tcgcgcacct cccaatcctt ggcgcccgcg
      421 gaaggcgccc ataccaaaag cgccacgccg ttgccgccgt cgcgccacac cagcgtctcg
      481 cccgccgagg tcgatgcgcc cagtcgatcc gactcgatta acggcgcggg ctcgctctcg
      541 aaatttccgg ccggctgcac gaagatgccg acgcgaaggt cgtcgagcgc cgtcttgccg
      601 gcccgaaccg agatcgcggt ggccgccatc gcgtcgatgt gcgggagcga ccacgcgtac
      661 agctcctgcc gaatctcgat gccggaactg cccttggggc cgtacaccgc gaccgacatc
      721 tcgtctccga tggcggcgaa gtcttcgtcg acgcgaccgt ccccgtcgtt gtcggcgcga
      781 tcgagcgggt cctcgtcgat cttgccgtcg tagtcgtcgt cggggcgcgg gaacgggtcg
      841 cgcgctccgc cgcgcgcgcc ttccttgacc gggtcgatgg gagtggacag gagcgattta
      901 acgccgccgg ttgcgttcaa gagctcggtc tcgacgccac tctgcgcgcg cgcatacaga
      961 aggacgccgc ccttgtcgag aaacgaccgc tcgttggccg ggccgaagaa cttccacgcg
     1021 ccgctgggcg tgatcgcgaa ggtcacgcgc gccggggaca cgcataccaa gccgtcgccg
     1081 caggcgcccg ccgccgcgcg tgccggcatc acacccgcac ccgcgagccc gcaaagcaca
     1141 agcggcgcga gtcgcgccgc cacgatcaac cgcttggtgt ccatgacgct cacatcgaaa
     1201 ggtagaagct caggctctcc agcgccaccg cgagattgac gttacggagc cacacgccct
     1261 cggggacggc gagctgcgtg ggcgcgaaat tgagaattcc cttggttccg cttcgcacca
     1321 acagatcgac gatgtcggcc gcggcctcgg gcggggtagc gaccaccgcg atgtggaccg
     1381 ggtcgcggcg caggacgtcc tccgcgtggc gctggtgggt gatctcgacg tcgttccact
     1441 tcgtccccac cttggcgggg tcgacgtcga acacgtgctt gaagaagaag ccgtcgtcgc
     1501 gaaatccttc gtggaagaag agcgcgtggc ccagcgagcc cgcgccgaag agcgccatgg
     1561 tccattgctt gtcgagcccc aggatctcga cgatcgcgcc cttgagctcg gtgacgctgt
     1621 agcccagccc gcgcgtcccg aaattcccga aatacgagag gtccttgcga acctgggcgc
     1681 tcgtgatgcc gcccaattcg gccaagcgcg cgctcgaaac cgtgggaata ccgcgcttct
     1741 cgaggtcgag caggatccgg tagtaattcg agagccgccg gaccgtgaac tcggagatct
     1801 tctccatctt cagacgcacc tacgcttcgg cctcttcttc catcgccaag tgggcgatgt
     1861 cgaccacctt gtcgtcgcca tccagattga tgagccgcac gccctgcgtg ttgcggccga
     1921 tcaccgagat gccggagatc gggcagcgaa tcacgatgcc gttcttggtg atgatcatca
     1981 tctggtcgtt gtccttgacc tcttggatgg tgaccacctt gccgttgcgc gcggtcgtct
     2041 tgatattgat cacgccgatg ccgccgcggt tcttgaccgg gtaatcctcc agcggcgagc
     2101 gcttgccgaa gccgttctcg gtgaccgtga gcagggtggc gcccgcggcc accaccacca
     2161 tgccgaccac ttcgtcgtcg ccgaccaggg tcgtgccttt gactccggtc gccgcgcgac
     2221 ccatcgcgcg cacgccgctt tcgtggaagc ggatcgagcg cccgttcttc ttggccagca
     2281 cgatgtcctg gccgccgttg gtgatggcgg cgtcgatcaa ctcgtcgccg tcgcgcaggt
     2341 tgacggcgat gatgccgccg cggcgcgggc gaccgtagtc gctcaatacc gtcttcttga
     2401 tgacgccctt cttggtcgcg agcacgagga actgatcctc gcggaactgg gagaccggga
     2461 tgcgtcccgc gatgcgttcg ccctcctgca gcccgagcag gttgacgatc gccttgccgc
     2521 gcgcggcgcg tccggcttcg gggatctcgt gcaccttgac ccagtggcag cggcccatgt
     2581 cggtgaagaa caggatgtag ctgtgcgtcg acgctacgaa gatgtgctcg acgaagtcct
     2641 cgtcgcgcat cttgatgccg gtgaccccgc ggccgccgcg cttctgctgg cggtaggcgt
     2701 ccagcgggag gcgcttgatg tagcccgcgt gcgagatcgt gatcaccatg tcctcttcgg
     2761 cgatcaggtc ctcgacattg aagtcgaccg ggcccgaatc gaggatctcg gtcttgcgct
     2821 cgtcgccgta cttctccttg atctcgagaa tctcggtctc gatgatcgaa agcaccttct
     2881 tgcgatcggc caggatcgac ttcaattccg cgatgagctt gaggagctcc tggtactcgt
     2941 cctcgacctt ctggcgctcg aggccggtga ggcgctggag gcgcatatcg aggatggcgt
     3001 tggcctggat ctcggagagc ttgaacttgg tcatcaggcc cgagcgcgcg gtttcgacgt
     3061 ccttcgaggc gcgaatgagc gctactactt cgtcgatgtg atccagtgca atgcggaggc
     3121 cttcgaggat gtgcgcacgt tcctctgcct tgcgtagatc gaacttcgtg cggcgcacga
     3181 ccaccgcttc gcggtggttc aaccagtgct ggatcatgtc gtgcagcgac aagacctcgg
     3241 gccggttgtt gaccagcgcc agcatgatga tgccgaaggt cacctgcatc tgggtgtgct
     3301 tgaagagctg gttctcgacc acgcgcacgt cggcgtcgcg cttcagaatg atcacgacgc
     3361 gcatgccctc gcggctggat tcgtcccgga tgtccgcgat gtcggagatg acgtcgtcct
     3421 tgaccagctc ggcgatgcgc tcgatcagct gcgctttgtt cacctgatac ggcagctcgt
     3481 cgatgatgat cgcttcgcga tcgcccttca tctcttccac gtgggtacgc gcgcgcacga
     3541 tcacgcgtcc acggccggtg cgatacgctt cgctgatgcc cagtttgccg agcacgatgc
     3601 cggcggtggg gaagtcgggg cccttgacga tcttcaagag gtcgtcgtcc gcgacttccg
     3661 ggttgtgcag caccgcgatg ctcgcgtcgc agatctcgac gatattgtgc ggcgggatgt
     3721 tggtcgccat gccgaccgcg atgccggccg caccgttgac gaggagattg ggaacgcgcg
     3781 agggcatgac gagcggcatg gtgcgcgact cgtcgtagtt ggggccgtag tcgacggtct
     3841 ccttgtcgag gtcggcgaga atctcttccg cgaaggcggt catgcgcact tcggtgtagc
     3901 gttcggccgc ggccgggtcg ccgtcgacgg acccgaagtt cccttgtccg tcgaccaacg
     3961 ggtagcgcag cgcgaattcc tgcgccatac gcacgatggt gtcgtagacc gcggtggtgc
     4021 cgtgcgggtg gtagttaccg gtgacgtcgc cggtcacctt cgcggacttg cggtaggcgc
     4081 gcgtgtgcgt gagcgaaagg tcgttcatcg ccgtcaggac gcggcgatgc acgggcttca
     4141 ggccgtcgcg cacgtccggc agcgcgcgcg aaatgatcac actcatcgag tagtcgagat
     4201 acgagttccg catctcggat tcgatgggta ccggcacgat cctctcgttg ttggccattc
     4261 gattcgctcc tagaagattt cttggcgtcc cgacgccttg tacctaacgc ggggacctcc
     4321 cgccgaacac gctctccgac ccggttgtga aaggaatttc aatgccccaa aataatagag
     4381 ggttcggggc cgaatgggaa ctgttttttt gcccccgtca ccgatgtaac cgagagtgtt
     4441 aattaaggtt acggtgtcgg cggggggccg tcgggagggg attttcggcc cacgcgtcgc
     4501 ttggcttcgt ccaccgccca tcgcgcggtc tgtttgatgc gcgccagcca ccgcttcgcg
     4561 aacgcgaatt cgagtcccag gatggccagc ccgcccacga tcgtgagcaa ccccggtccc
     4621 gggagcacga gcatcgcgat cccgagcagc acgacggtca cgccgatcac cgcgatcacg
     4681 atgcgtcgcg cggtgcgata ggtgagcttc cacgtttgct tgactcgacg gtccacttct
     4741 acctcacgtc gattcacgcc gctcgcagcg cgaacgatag tgacactcga atcacgattc
     4801 catagtatca cccggatgtc aaacgcccgc ggcccatgga actaaagcgc ctcgcgcact
     4861 acgagatcgt cggccccatc ggggccggag gcatgggcga ggtctatcgc gcacgcgata
     4921 ccaagctcgg ccgcgacgtc gcgctgaagc tcttgccgga gcacttcgcg cgcgacgccg
     4981 atcgtttggc gcgcttcgag cgcgaagcac gtctgctcgc atcgctcaat catccccgca
     5041 tcgccgcgat ctacggcctc gagcaggacg gtgcccaccg cttcctggtc ctggagctca
     5101 tcgagggcga ggatctctcg aaacgaatcg cgcgtggtcc ggttccgttg gacgacgcgc
     5161 tggggattgc gctgcacgtg tgcgaggcgc tggaagccgc gcacgaaacg ggcgtcgtgc
     5221 accgcgactt gaagccgagc aacatcgtcg tgacgccgaa cggatccgtg aaggtgctcg
     5281 atttcggttt ggcgaaggcg ctcgatccgg cgtcggggaa ttcaaacctt acacaatcgc
     5341 ccaccatcct gggcagcagc ccgacgatgc agggcgtcat cctcggcacc gcggcctaca
     5401 tgagtccgga gcaggcccgc ggtcgcgcgg tcgataagcg ggcggacatc ttcgcgtttg
     5461 gttgcgtgct cttcgagatg ctgaccggta agttggcgtt ctcgggcgaa accgtgagcg
     5521 acacgctggc ggcggtgctg cgcgccgacc cggattgggg cgcgctgccg aaagataccc
     5581 cggcggcgat cgcgcgcctc ttgcgccggt gcctcgaaaa agaccccaag cggcggctgc
     5641 gcgacatcgg cgaagccgct ctcgtgatcg acgatgtgcg atcgggccgc atcgtcgatg
     5701 aacccaccgc ggtcgctgcg ccctcaatac ccaaggcgcg ctttgtgggt ccgcgcgcca
     5761 ttgcggtggt ggccatcgtg ctcgcggcgg tggcggccgg gtcgagcgcc ctcacccgcc
     5821 ttctttcgcc gtcggaagcg gaaccgccgt tccggaagct cctgctggac gtcgtgctca
     5881 ccgactcgct gggtgcgccc gaggcgcccc gcatttcccc cgatggcaag cggctcgcat
     5941 ttcactccgg cggtcgcctg tggattcgcg acctcgacaa tttggagccg cgcgcgatcg
     6001 cggagtccag ctcggaagtg gccccgtttt ggtcgccgga cggcgcattc gtgggctacg
     6061 tcacctcgca gcgcctgtgg aaggtgcgcg cagccggcgg tgaccccact cccgtgtgtg
     6121 attcccccgc gagttttggc gggggagcgg gggcgtcatg gggcgacgac gggcgcatcg
     6181 tgttctcgta cggaaacggc ggggtctacg aagtctcgtc gagcggcggc gacgcgcgcg
     6241 agatcatccc cgtcgacgtc agtgtcgagc aagatatcca tcagcccgcg atgctgcccg
     6301 ggggcaaggg aatcctctac atccaacacc gccagcagtc cggcccggat actattcaat
     6361 tgttcgcgaa cggaactcgc aagacgctgc tgacgctgac cggccagaat ctctggtcgg
     6421 tcgagtacgc gccgagcgga cacattctct tcgtgcggat ccccgacaac gcgggactgt
     6481 gggcggcgcc gttctcgctg cagtcgctcg agatcaccgg tgagccgttc ttgatcgcgc
     6541 ccaaatcgag cgagggcagc gcgtcgaacg acggcgcgct ggcctacctg cccaccctct
     6601 tcggagacat ggtccgcgtg gcgcggtacg acggcgccgg caagttcgtt tcctacgcta
     6661 ccgaaacttt gaccaacgtc aactcgatcg cgctctcgcc cgacggttcc aagctcgcgg
     6721 tgtcgatgcg cgacgccgcc acgcgtgatg tctgggtgat cgacctcgtc cgcaacacgc
     6781 gcagtcgcct gacgttcgcc ggaaactaca acacccaacc cgcgtggtcc ccggacggca
     6841 agacgatcgc gtatcgcaac gtcgacgatc agagtctcta tttgatcccg gccgatggaa
     6901 cgggcgaccg gcgcttcctc acgcgcggcg cgagctttcc ctgctattcg cccgacggac
     6961 gctggatcgt gttctcgacc cgcctgagcg gaagcgcggg caaccaggat tccgacctgt
     7021 tcatggtgga tgcgtcgctg acggacacga cgcgcgtcac cgtgttcgcg ggaaccggcg
     7081 accagcggta cccggtcgtg tcgcccgacg ggaactatgt ggcttacctg tccaacgaga
     7141 gcggcaacga cgtagtgtac ttgacgacgt ttccgcgctc gaccggcaag tggcaggtgt
     7201 cggtgcacgg cgacgcgaac tggccgagct gggcgccgac cggagaccgc atctatttcg
     7261 tcgccgacga ccgcgtggag atggtcacct tcgagccggg agcagcaccc cgcttgggta
     7321 cgccgcagaa gatgctcgac accacgcgcg cgcgcctcga gttgtggggg ttccaaaagc
     7381 tcgacgtcga tatcgacggc cggggaatac tggtggtgga ggcgacgcgc agcgtcgagc
     7441 agcaggacgg cgtcgtgttc gtggaaaatt ggctcgagga gttttcgcag gatcgatagc
     7501 ggcgccgttc tcgatcaccc gcgttcgcgc aagacccgcc cgattcggga caatgcttcc
     7561 cgcatgagat cgctctcgag accgaacgag atccgaaaat gatcgggggc gccgaagaac
     7621 cgccccggga ccacggccac ctcgaactcc gcgtgcaatc gatcgaccag ccggtcgacg
     7681 tcccgatctg cgacctgaac gaagccgacc gtccccgcgc gcggcttggc ccacgagagg
     7741 tcgtcgtgcg atcgcacgaa ggcgtccacg agctcgatgt tgcgcgcaag gagcgcgttc
     7801 gattcggcca agagcgcatc ggcgtgctcg agcgcgcgcg ccgcgatgcg ctcgctgggg
     7861 tgcgcggtat tcacggagaa gagatcgttg aggcgtcgca tgcgctccgc aagctcgggc
     7921 tcggcgagca cccatcccag gcgcagtccg tcgagcccga acaccttcgt catgctgctg
     7981 gtcgcgagaa tacgcggcga ctggccaacg gcactacgcg cgccgcgagc atggaagaat
     8041 tcgagataga cctcatccac caggacgcgg gccccgacac tctcggccgc gcgcgcgaca
     8101 tcggacagcg tatcctggtt atcgaaggcg ccggtcgggt tgtgcagatt cgataccacg
     8161 acgagcttgg tcttgggtgt gagcgcgcgg cggacttctt cggggtcgat atgccagcga
     8221 ttctcggcgt ggcgcgcgaa ccaaatcacc cgcgccccga gatactccgc gacgcgcacg
     8281 agcggctcgt agaccggggt ttccatgacg acgtcgtcgc cggcttcgag gatcgcggcc
     8341 atcgcgaggt gattcgccat cgagcagccg tgggtggtga cgacgtgcgc gggagtcaac
     8401 tcctggcgcg ccgcgattcg ctccatcagc ggaggccagc cgtcttcggc gttctggtta
     8461 tgtagaagat cggaaagtgt cagctcgagg cgctcgagcg caagccgggg cacgccgctg
     8521 cgcgccaggt tgtagcgcac gggcacgcgg cgtttggtcc aggtgagata gtcggagccg
     8581 ctggactgtg ccggccacgg cgtcacgggt gtgctttcct tgcggcccgc gttacacgtc
     8641 gaggttcttg accgagagcg cgttctcttg gatgaactcg cgcctcggct cgacttcgtc
     8701 gcccatcaag atcgagaaga tgcggtccgc ttcgactgcg tcctgcacat cgaccttcaa
     8761 gatggtgcgc gcttccgggt ccatggtggt ttcccacagc tgttcgggat tcatctcgcc
     8821 caggcccttg tagcgctgga tgtagacccc gtcgttgccg ccccaatcct tcagcatgcg
     8881 gtcgcggtct tcgtcggcgt acgcgtagac ctctttcttg cccttcttga gcttgtagag
     8941 ggggggctgc gcgatgtaga cgtagccggc ttcgaccagc ggcttcatgt gccgatagaa
     9001 gagcgtcagc aagagcgtgc ggatgtgggc gccgtcgacg tcggcgtcgg tcatgatgat
     9061 gatcttgtgg tagcgcacct tggtgaggtc gaattcgccg ccgccggtcc cgaccgccgt
     9121 aattatggtg cgaatctcct cgttggagaa gatcttgtcg aggcgcgcct tctccacgtt
     9181 gagcaccttg ccgcgcaggg gcagaatcgc ctggtattcg cggttgcgcg ccatcttggc
     9241 cgagccgccg gcggagtcgc cctcgaccaa gaacaattcg ctcacggccg ggtcgcgcgc
     9301 ggcgcaatcg gccagcttgc cggggagtgc cgcggattcc agcgcggtct tgcgacgggc
     9361 gaggtcgcgc gccttgcgcg ccgcttcgcg cgcgctgaac gccgccagcg tcttctcgac
     9421 caccgagcgc gcgatggacg gattctcttc caggtactcg cgcagcccgt ctcccaccac
     9481 tgcttcgacg atgcctttga cttcgctgtt gccgagcttg gtcttggtct gtccctcgaa
     9541 ctgcggttcg gccagcttga cgctcacgac cgccgtcaat ccttcgcgca cgtcgtcgcc
     9601 gcccagcgtc acgcgttcct tcttgaagag gttcgcggac gacgcgtagt tgttgatcgt
     9661 gcgcgtgagc gcggccttga acccgaccaa gtgcgtgccg ccttcgatgg tgttgatgtt
     9721 gttggcgaac gagaatacgt tctcggcgta gccgtcggtg tactgcagcg cgacttcgac
     9781 ctggacgccg tctttctcgg cctggatgaa gatcggctgg ggagtcagca ccgttttcgc
     9841 ggtgtcgatg tacttcacga acgacacgat gccgccttcg tactggaaca cctgccggcg
     9901 cggcttgccc gcgcgacggt cgtcgaatac gatcttcaag cccttggtca ggaacgcgag
     9961 ctcgcgcagg cgttgcgcca gggtttcgta ctggtagtcg accttttcga aaatctcgct
    10021 gtccggcatg aaggagacga tggtgccgcg acggtcggtg gtgccgagct ctttcaggtc
    10081 gctggcgggg atgccgcgtt cgtagcgctg cgtgtagcgc gtgccgtcgc ggtggatctc
    10141 gacgtcgagc cactcggaga gcccgttcac gaccgaaacg cccacgccgt gcagaccgcc
    10201 cgagaccttg tacgagtcgc gatcgaactt gccgcccgcg tggagggtcg tcatcacgac
    10261 ttcgaccgcc ggccgatgct gggtgggatg catgtcgacc gggatgccgc gcccgttgtc
    10321 ttccacgctg acgtggaggt cctcgtggat cacgacttcg atgtcgctgc aatgcccggc
    10381 cagcgcttcg tcgaccgagt tgtccacgac ttcatagatc aagtggtgaa cgccgtggat
    10441 atcggtgcta ccgatgtaca tcgcggggcg cttgcggacc gcttcgagtc ccttgagcac
    10501 ctgaatctgt ttggccgtgt attcgtgcgt catctcgacc tttcccgtct tcgatacgtc
    10561 cgattcgcct gcgggaatcg gaggtgtgtc tttcgtcgcg gaggacttgg ttttttccag
    10621 catgatgtcc gcccgtctat tccatgtcgc gcgcgcccag ccggatccgc gtcaagttga
    10681 cgtccgggca ggctgctcgc agtttcttga cgagctcctt ctcgatgaaa tggagctcct
    10741 gcatccacgc gccgtggtcg acgcggacgt acagggtccc gttctcgtac cgcgtggccc
    10801 gggcgcgctg cgagatttca gggccgacga tgtcggccca tcgctctacg agctgtcgct
    10861 cgctgaaccg tttttggagc ccgaggtcgc gcaggacgga tgggaggagg tctccgatcc
    10921 gagcgaagga acgccgcgtc atacgcctga aaggtactgt gtttgcgcgc aataatacac
    10981 cctttttttg cgcgccctgg ggttgtaacc agttgtggat cttaatgtta caattggtaa
    11041 ccgggtcctt aacgttccgc cgtcacgcgc tcgagatgcg ccccgcccaa acgcggtgca
    11101 cgcgcgctcg cgcatcgctg gcaaggttcg ccggactggt gacgaagacc tggcgggaac
    11161 catcgaggaa tcggtgcagc ttggcgatga tggcttcgtc gaactcggaa aagacgtcgt
    11221 cgagtagcag tacgcacggc tcgcgcagct cgttctcgag gtacgacgtc tccgccagct
    11281 tcagcagcac cgcgagcagc cggcgctgtc cttgcgatcc gaagcgtcgc aggtcgtggc
    11341 cgtccatcac cagttcgacg tcgtcgcgat gggggccggc gagtgtgacc ccgcggcgat
    11401 actcttcgga agcgatcgag gccagcgtgt cttgaaatgc gcgggtgagc gacgcggcgt
    11461 cctcggtatc cgcgagcaga tccgaatcgt agcgcatcac gaaatcgaag ggactttgga
    11521 gatcgctcgc ttgcgcacgc atctcgcgct ccatggctgt ggtcagcgcc cttcgccgca
    11581 atatgaaatc ggcgccgagc tcaatcaggc ggtcgttcca ggccgccatc tcgtcacgcg
    11641 aggcgcgcgc tttcaaggcg gcgttgcgct gctcgacgac gcgacgatac ttggtcgcgg
    11701 ttcgcaggta accgggatcg gtcatgatcc cgatcgtgtc caagaagcgt cgccgattct
    11761 cgggctctcc cgagacgatg cgcaggtcgt gcggtccgaa gagcacggcg gggaagcggc
    11821 cggcgaggtc gccgcgccgc gggagaagct ccccgttcac cttgatcgcc ttggccgcgc
    11881 gcccctcgcg gcgctcgatc gagaacgcga agtcttcgcg gcgcccgtcg gcgaaagtgc
    11941 acgcgagctc cacgcggcag aacggcgcat cgaagcgaat cagctcggta tcgacttgtg
    12001 ctcgaaacga tcgcccgaac cggaagaaat agacggcttc gaggaggttg gtcttgccct
    12061 ggccgttgtc gccgaccagc acgttcaggc cgggaccgaa tgggatctcg ccctcgagga
    12121 gattgcggaa gttcgtggag gcgaatcgat cgacgcgcac ggcgcccgct cgctcagtcg
    12181 ctcaagcgca gaggcatcaa caagcactcg tgcttgatct cctcgccgtc ctgctccggt
    12241 gtcaccacgc ccgcgttgtc gttgcgatcg agcaggaacg acacgtccgg gctctcgacc
    12301 gtgcgcaaga tgtcgaggag ataacgggcg ttgtagccga tctccatctt gggagaagtg
    12361 tagctgcacg tcacttcttc gagcgcctca ccgacgtcgg gcgtcttcac cgacaaggtc
    12421 aatcgatcgt cttcgatcac cagcttgact tggtgcgtga tcgcactcga gagaatcgac
    12481 acgcggcggc acgcgtccgt cagctcgtcg cggctcaccg tgagtttgcg cgtgttcttc
    12541 ttgggcatga cctggttgta gtcggggaag gggccctcga gcagccgcga gtagatcacg
    12601 ctgtcgccca gatcgaacgt gatgtggttc tccgccagcg agatgtgcac gctgtccatg
    12661 tccgccgcga gcgagcggta gatattgagc gccttagggg agacgatcac gtctttctcc
    12721 tcgccgccgc cgactgctcc gggaagctgg accttggcca ggcgatggcc gtcggtcgcg
    12781 atcatgccca gcgcgccctt cttgatctcc cacagcacgc cgcacaacga cgggcgcgtc
    12841 agatcggtcg acaccgcgta gctggtcttg ttgatcattc gatcgaggac gctcgcggcg
    12901 atcggaaatg tcacggccgc cttctgggac gggagcttcg ggaattcgtc ggcgctcttc
    12961 ccggtcatct tgaaacggct gcgacggcac ttgatggaaa tgcgatcgcc ttcgacctca
    13021 atgactactt tgtcgtcggg tagtgagcgc gcgatttcga agaacttctt gcccgggacg
    13081 gccacacttc ccggccccga cgtttcgcac gcgagtgtct gtgtgagcga tacgtcgagg
    13141 tcgtttgcgg tcagggtgat cttgccgtct ccgcgcgtct cgagaaggaa cgtcgaaaga
    13201 atgggaagtg atgtcttgct cggcacgacg gccgcaatac cttgaaggcc cttcagtaga
    13261 tccttctgat cgacggtgat tttcactccc ggtcctcctt ctcgccccat ggatcaaaac
    13321 cctcaccgca gcgcgattga caaagctttg tgcggagccg cgactatacc gcagtcgaag
    13381 gtcccttcgc aaggaagctg tggctgttgc gaacaggttt ttcggttcct ggttacttgt
    13441 ggtgccatca acccgggtct cccgggatgg gtaaccgtgt ggtgcccttt ccgcgcgtgc
    13501 ttcctactga tggatcgaat gatatatcca atcgcagtag cagtagggtt gtggagatgt
    13561 ggaacaaaga agagcaacca agcctggtcc agcgcgttgc acggtgtgaa cgccgtggac
    13621 ggaccgggga gtgtgtgttg aaaagaccgc gaaccctccc catgggataa cttcccacac
    13681 actatccaca cgagatatcc cgagtattgt ggagaaacca aagggtgaca acgtgacagc
    13741 ccgactcgta ccaatatgca aagaggccgg gaagtgggcc atgaatccgc cgctccagcg
    13801 agcgaatccg cgtcccgtcc ccggcccctt tttgccgcac acctctgtgg ctagcgaact
    13861 cttacgacga aagctccttc cgaagctgcc caatggtcga gcgaagcgag acgtcgcgct
    13921 cgatcatgtc ggagatcttc tggcaagcgt ggatcacggt ggtgtggtcg cgcccgccga
    13981 agcgctgtcc gatctgcgcc agcgacgcgg acgtcatgtc gcgggcgagg aagatggcta
    14041 cctggcgcgg gaacgcgagc cgggccgtgc gcaccttcga ccgcatcgac tccacgggga
    14101 cccggtagtg ctgcgacacc accttctgga tcaaggtgat gctggtcttc ttgggcgtgg
    14161 acttgatgaa gtccttgaga acgtcgcgcg cgatctcgcc ggtgatctct tgtttgtaca
    14221 cgctcgcgtg caccaacaac ttgaccaggc tgccctcgag ctcgcggatg ttgctcttga
    14281 ccgaatcggc gatcaaggcc aggacatcgt ccgggatgga gatgttctcg cgctccacct
    14341 tgcgacgcag gatcgcgagc cgcgtttcgt agtccggggg ctggatgtcc gtgatgagcc
    14401 cccactcgaa gcgcgatacc aatcgatcct cgagggtcgg gatctccttg ggaggccggt
    14461 cactcgtcac cacgatctgg cgattggcgc cgtggagcgc gttgaaggtg tggaagaact
    14521 cctcctgcgt gctctccttg ccggccagga actggacgtc gtcgatcagc aagaggtcga
    14581 cgttccggta cttggtgcga aactcgtgac cattcgcctg gcggatcgcc atgatcattt
    14641 cgttcatgaa cgattcggcg gacgagtaat ggacgcgcgc agtcgggtgg ctcttcaaga
    14701 ccacgtgacc gatggcctgc atgaggtgcg tctttccgag cccgacgccg ccgtagatga
    14761 aaagcgggtt gtacacctgc gcgggccgtt ccgaaaccgc catgcacgcg gcgtacgtca
    14821 tgcggctgtt actgccgacg acgaattcgt cgaagacgta cttggggttg agccgctcac
    14881 cctcgagcgc gggcttgcgc gcgcgcggcg ccatgggcgc gggaacgagc tcggcgcgcc
    14941 gcggcgagat atcgggtggc gcgtcgttcg cgttatggag cgagatctgc gggtcggttc
    15001 ctagaacctg ccgcagcgca aaagcgattt tctcggagtg gtgctcgctc acccaatcga
    15061 cgaagaactt gttcgggcag tcgaggacca ctttggatcc atcgaccgcg ttcaagcgca
    15121 tcggcttgaa ccacgtttcg aacgtttgct tgttgaccaa cgtggccacg gtagccagga
    15181 actcggacca catgtggtcg cgggaggaaa ccgacatgga tagtaccctt tctccctcgg
    15241 agtgactccg atgcgagacg gccggaatgc gaccgaaaac gcaatccctt caccaacatc
    15301 gttgcgatca ccgtcaccgc gcggctgcgc gcgggcgaaa actcttgaaa gaacgccggt
    15361 tcggacattc cccggcggaa ccgaagggcg ttatccacaa atgtggataa tcacctaatc
    15421 tattgtggac aagcgggatg cgaggcaaaa aggggccgtc ttcgcgaagt tgtccacaga
    15481 ccgtgactca aagaccaagc tgaattaatc gggaaagacc aaagccccag accgaagatg
    15541 caacggccgc gaaggtagca aagtgtgaag aagcgcttca agagcgaaaa cgagttgctc
    15601 acaggaacag acaagtcgcg tttcgaaagc cggttgcgcg gcgattgcct gtggataaga
    15661 tgtgggtttc ctcctccgcg acggggcttg acgacgccgc gtgcttggtc taaagtcacc
    15721 cccttcggtc cagtatgcaa gcccggcacg cgcgagcggc attcggtcgt ggtcgccggc
    15781 gggcccaacc aaacgaacct agggattcac gatcatgaag cgcacatttc agcctcgcaa
    15841 cctcaaacgt aaacgcgagc acgggttccg caagcgcatg agcacgcgcg gcggccggct
    15901 cgttctgaag agacgccggg cgaagggccg caagcgcctg gccgcgtagc gagaaccgaa
    15961 ggaccacgga cgtgagcccg gccaagcaac cgtcgggcgc agaccggggc cggtcggctc
    16021 acgcgaagcg gcgacgcgtg cggaccttga cgcacgcctg gcagtttcgt cgctgctacg
    16081 attccggaaa gaagctcgtc gcccgacacg ccgtgatttt cctccatccg tccgaagcgg
    16141 atgatggact gcgcgtcggt gtcgtcgcca gccgcaaggt cggcggcgcc gtcaggcgaa
    16201 accgtgcgaa gcggctcttg cgcgaaaccg cgcgccgcat cgctccaaaa tggattgatc
    16261 gtcacatatg ggtcgtcctg gtcgcgaagg cgtcgatcgt cgatgcgcgc gccgacgaga
    16321 tcgaggacga catcgggcga gcgctcatcg cgggtggcga gctcgacatc gaataatcgt
    16381 cacaggaagt gatcgtcaca tgcgattccc gcccgctgcg atgcttcgcg catcgctctt
    16441 ggccttgatt gccggctatc gccggctggt ttccccgctt ctgcctcccg cgtgccggtt
    16501 ccatccgagc tgctcggaat atgcgtccga ggcgatcgcg cgccacggcg tggttcgcgg
    16561 cggcgtgctc gcggccggcc gcctcgcgcg ctgtcacccc tggtgcgagg gcggtatcga
    16621 tccggttccg cctgctcgtg gctcgcgcga ggcgaatgcc cgcgcgcccc gcgtggaagg
    16681 tgtccgatga gcaagacctt cgggatggaa cgccgcgcgt tgctcgcgtt cgcggcctcg
    16741 atggcgctgt ttctcttgta cgacgcgctt tatctcgcgc ccaagatgcg cgaagagcgc
    16801 gcgcgccgcg aagccgtcca gctcgaagag gcgcaaaaga tcgcgcgtct ccagaaggtc
    16861 gacgcgtccc gcgacacgct gtcggtcgga ggcgaccagc ctgcgctggt gccggcgccg
    16921 gcgggagatt cgctttcgat ggcgtttgcg cccgccgata cgggcatcgc cgcggacgaa
    16981 ccgtcccagc gcatcgtggt ggcctctccg ctcttcgaga tcacgctcga cacgcgcggc
    17041 gcgtccatcg aaggggttca tctcttgcgc ttcgccggag cgaacggacc ggtcgagctc
    17101 ttgccgccgg gcgacagctg gtcgggcgat cgcatgttgt cggcgaccat tcgcgggtcg
    17161 gcgcgcgagc tcgcggtcga gaagatcgtg ttcgaggcga gcgccgggcg gcaggcgcta
    17221 tccgacggcg cccgcgtgaa tgtcgccgcc ggcgactccg tcgacgtcgt atttcgcgcg
    17281 gcgggtccgg gcggcatgat cgagcgcgcg tatcgtttct acggcgaccg ctacgatttc
    17341 cgcaccacgc tgcgcgcgag cgaagcgctc gtccccggcg ctgaaacggt ggcgtggagc
    17401 tttggtcccg gactcaccgc caccgaaaag aacgcgcgcg acgaccagtc gaaattccgt
    17461 gcgtcggtgc tcctggggca ggaattccac cgcaagcacc ccggcgactt cggccgcgcc
    17521 aacgtcgaaa aattcagcgg gacgctcaac tgggccagcc tgcaaaccaa gtattttcta
    17581 gccgccgtca tcccgccgca gccggtgcgc gcggagatcg agatgtcggg gatcaagtcg
    17641 gagcaccgcg tctcgcagag cgtggcgatc ccggccacgg ttgcgcgcgg cgagatcacg
    17701 agcgagttgc gcggattctt cggaccgctg gacttcaagc tggtcgaagg cctcggtgtc
    17761 ggcctcgaga agaacgtcga gttcggctgg aagttcatcc ggccggtcag tgtggcggtc
    17821 ttgtggtcga tgaacgcgct ccacaaggtg attcccaact acggctgggt gatcgtcatc
    17881 atctcgattt tgaccaaggt ccttttctat cggctcaccc acaagagctt caagtcgatg
    17941 aaggagatgc aggatctcca gccgcgcatc gcggcgctca aggagaagtt tggcagcgat
    18001 cgtcgtcgct tgagcgaaga gacgatgaag ctgtacaagg aagccggcgt caatccgctg
    18061 ggcggttgcc tgccgatgct gctccagatg ccggtcttca tcgccttgtt caacgtactg
    18121 agccacgcca tcgagctgcg cggcgcgccc tggatcgcgt ggatcaacga cctctcgcag
    18181 caggacgtgc tgttcaagct gccggcgtcg ctgccgctcg tcggcgacgc tttcagtctc
    18241 ttgccgctct tgatgggtgc gtcgatgtgg gcgcagtcga agctcggagg ctcgccgacc
    18301 ggccagccgg gcaatccgat gccgcccggg ttcaacacga tcctcccgat cgtgttcacg
    18361 ttcctattct acaagatgcc gtccgggctg gtgatctact ggatcatcaa caccgctctc
    18421 tcggtggccc agcagtacta catcgtgaag agccacaagg cgccagcact cgccgtggtg
    18481 ccggagtcgg ctgcgcccag cggaaagaag cgccgcgccc gcaagagctc gtaggcgaat
    18541 caccatggcg cattccgtcg agaccaccgg caagaccatc gaccaggcgc tcgagaaggc
    18601 tctgaaggag ctcggcgcgc gcaaggacga ggtcgacatc gaagtccttt cggagacgca
    18661 cggcggcctg ctgggcatct tcggggggaa gatggtgcgc atccgcgtga cctccaagaa
    18721 cgcgaaggcg cccgcgggtg ccgcggaagg cgccgacgtg gtccgctccg tgatcggcga
    18781 cgtgctcgga ttcatgggcg tcgaatacac gctttccatc gaagaatccg acgacaccac
    18841 gttcgtgaac atcaactcga gcggactcga tggattgctg atcgggcggc gcggcgaaac
    18901 cctcgcgtcg atccagcacg tggtcaatcg catcttcacg aaacggaccg gccgccacag
    18961 caaaatcacc gtggacgtgg gcggctacgt gcgacgcaag caccgcctcc tggtggagaa
    19021 agcgcagaag ctcgccgagc gcgtcagcaa gacccagaaa gagtacgact tcgaaccgct
    19081 caaggcgtcg gagcggcgca tcatccacct cgcggtggcg gacttcgacg acgtgacgac
    19141 gtacacgatc ggcgacggtc tcctgcgcaa ggtcgtcatc tcccccaaga cgtcggcgac
    19201 cgacgacctg ccgcgggatt gaggctgtcg ctgcgggcga gcctcgcccg gttgcgcaga
    19261 tgcttcgccg ttcgtagatt gcgtccagca gacctcggct cccctgccgt cgtgaatccc
    19321 atgcgtggtc ccgtgcccat cgttgccctg ctggctatgt tgcttccgta cgccgtggaa
    19381 gccgcgagcc tgttgggtgt ggtgcgcgcg cgtgacaccg gcgcgccgat cggcttcgtc
    19441 aatgttcagg tgtccggccg ctcgcaggcg ggagccgtca ttcaaggcgg ggtgctcgcg
    19501 ggcgagaacg gcagctacct catcgagccg ctcccgctgg ggcgataccg cgtcgcgttc
    19561 acgcgcgtgg gctatcgcgc gcgcgaggac agcgtgatcg tggccgaaga gcgaacgtac
    19621 gtcctcgata tcgcgctgga gctcgagccc gtgcagatcg agggcgtgcg cgtcgaagcc
    19681 gacacggatc gcgagttcac cgaaaccgaa gacgtgcaga ccggcttcgt gtcgatccgg
    19741 gccgaccggc tttcgtcgct gcccgggatc gccgagggcg atcccgtgcg cgcactggat
    19801 ctgtttcccg gcgtgcagtc cgcgtcggac ttttcgagcg cgctgtacgt acgcggcggc
    19861 ggacccgatc agacgctcgt gctcttggac caggccaccg tctacaatcc cacgcacgcg
    19921 ttcggattct tctccacctt caacgccgac gccaccggcg acgtgaatct gtacaaggga
    19981 gcgtaccccg ccgagcacgg tggccggctg ggcgcggtgc tcgacgtgcg gtcgcgcgag
    20041 gcgcgcgcgc aagaattcct cggcaaggtg gggatcagca ccatcgcgtc gcgcgttgcg
    20101 ctggacggcg cggtgcgaag cgggtcgtgg tcggtcagcg cgcgtcgcac ccatctggag
    20161 ccgatgctcg acgccattcg cgacgaggac accgaagtcc cggactattt cttctacgat
    20221 ctcaacggca agatctcgct gccgaccggc ggagggcgct tcgacgtgtc ggtctaccac
    20281 gggcgcgacg acctcgattt ctttcccggc ggcgacacct cgctcgagct gcggtggggc
    20341 aacacggtgg cgacggccgc attcatgcgc gcgttgagcg accgcctcat cgtcagcgcg
    20401 cgcgcgtcct ttagcgacta cgagagcaat acggtcgcga gtgcattcgc gacccccgcg
    20461 accttcgaga acgcggtgca agacatcacg gtcggcggcg acgtgaagtg ggaggcgagc
    20521 cttcgtcaca ccttcacggc cgggctgcaa gcatcgacct acgacgtcgc gttctcgcag
    20581 acgttcaacg gccaactggt cgacgactac cggcgcgagc ccgtcgaggt ggccgcgttc
    20641 ctcgaagacc gctggacgcc gtggggcgat accgcgttcg acctcggcat gcgcgcgcgc
    20701 tacatcgacg acggcgagcg ctatctcttc gagccgcgcg tcgcgttcgc gcaggtgttc
    20761 cggcccgacc tgcgcgtgaa agtgggggcg ggcatctatc atcagtacct ccagctcgtc
    20821 gcgaccgaag cggtcgcggc cgtggacttc tacgtcccca tcgacgagac ggcaccgctg
    20881 ggacgctcgt ggcaactcgt cgccggcgcc gactggcgct ggaaaccgtc ggtgttgttt
    20941 tcgatcgagg gctactacac cgacctcgag aacctcgtgc tgctcgacaa cacgtcgacc
    21001 gtgaacgtgg aggtcgaaac cgccgacgac ttgttctact tgggcggcga cggatacgcg
    21061 gcgggcgtgg agctgtttgc gcgccgcgac atgggctcgg tcaccggttg gatcggctac
    21121 acgctgggct ggacgcgacg cgccttcccc gagatcgaag acggcgagac cttcgcgccc
    21181 aagtacgacc gccgccacga tatcagtgcg gtggccgaac accggcgcgg cccgtggcgc
    21241 ttcacgggtt ccttcgtgtt cgcgaccggc caggcgttca cgcccgtttc cggccgctac
    21301 tcgctgcgcg acccgactac gggtgtcgtc ccgagcgaca tccagttgct ctacgccgac
    21361 aagaacagcg cgcgcctgct cgcgtacaac cggctcgacc tgggtgtcgc gcgcgacttc
    21421 tcgttcttcg gagccaaggc ggaatggttg atcgaagtgt tcaacgtcta cagccgccgc
    21481 aacgagtggt tcgtcgagta tcagcgcgac gaggaagtcg tcgacgcgga ggtcattcgc
    21541 atgttgccca ttattccgag catcgggatc aacgtatggt tctaaagcgc gcgcgcatac
    21601 tcgcagtggg catcgctgtg tgggcgctct cgtgctctcc ggagcgcgag ggcagcgagc
    21661 tgtttgcgcc cggaggcctc ggtattcccg tggtcgacgc ggtcctggtg gtggggcagc
    21721 cgttcagcga cgtctacctc tcgcagaccc tcgcccccga tgagcggttc acgcgcgagc
    21781 gggcgggtct caacggcgcg acggtgacca tcgaggacgg tgcgcgcgtc attccgtaca
    21841 cgtccatggg ggtgcgcggt tggtaccggt cgggtgcgcc cgacatcgtg caggcgtcga
    21901 cgacgtatcg cttcacggcg accctcaccg acggccgcgt cgtgcgcgcg acgacgacga
    21961 cgcccgcgcg tctctccgtt cgcgagtggg tactcctcga cgaatcgggc acaaacgtcc
    22021 tgcaaacgct cgcgaccttc gaagagcacg gcgacaccgt atacttcgaa ccgcagaacc
    22081 agctcgtgta tccggagaac atcctcgcgg ccgtcttgga cgaccaatcc accgccgggt
    22141 atcaaatcgg actcatcaac ctcgagacgg actcgccgct cttgatcgac gcggactttc
    22201 tcgagccgga cgacctcgag gacttcaagc ggaccaactc gtctccaccg ctcaattacg
    22261 aatcgacgct gcggatcccg tggctcgcga tctattacga ggggcggtac aacctgcgcg
    22321 tcgtcgcgct ggaccgcaat tggtacgaca tcgcgcgcac ggatccggcc atcggcggcg
    22381 ggggattcgg tttcgggggc gaagcgggag acacctcgaa gcgccccatc tttcacgtcg
    22441 aaggcggcat cggcgtcttc ggatcggtgt cgtcggactc ggtcggcttc tacgtgaacc
    22501 cgcccgcgca gtgaacgctg cgcggcgggt cctgtcgccg gtcaccccgc cgcctggcgc
    22561 ggcgggggcc ccaccggcga cacccgccgc gggatgctcg gagctatcgt gttagaatcg
    22621 taattcgtca tgcattccac caccgacacc atcgccgcac tctcgaccgc tcccggtgaa
    22681 gccgggatcg cggtcatccg cgtctcgggt ccggaggcgc tcggggtgct cgatcgcgtg
    22741 ctgcgcacgc cggccgatcg ccgcttcgag ggcgactggg agcaccgacg tctttatcac
    22801 ggcggtttct tcgatccagc gggcgagcgc gtcgacgatg tgatggcggc cgtgatgcgc
    22861 gcaccggact cctataccgg cgacgacgtg gtcgagatca gctgccacgg tggcagcgcg
    22921 gtggtggcgc gaatactcga gacgttgttc gcgagcggcg ctcggccggc gcagccgggt
    22981 gagttcacgc agcgcgcatt tctgaacggc aagatggacc tgatccaggc cgaagcggtc
    23041 gccgatatga tccacgcgcg caccgagctg cagcgcgtgg tcgccgagcg gcagctgcgc
    23101 ggcgcgctct cgcggcgcat cgacgcactc gccgacgaca tgatgacgct cctggccgtg
    23161 gtcgaggcga acatcgattt catcgaagag ggcatcgacg ccctggacgt cccggccgca
    23221 ctcgcgacga tcgcgcgcca gcgcccggag ctcgacgatc tgctctcgac ctcgtcgttg
    23281 tcgcggccgc tgcgcgaagg ctaccgcgtg gtcatcgcgg ggccggtcaa cgcgggcaag
    23341 agcagtttgt tcaaccgcct gacgggcgag ccccacgcca tcgtgaccga gattccggga
    23401 acgacgcgcg acgtcctgcg cgagacggtt gtgatcgagg gcgtgccctt catcctgcac
    23461 gacacggccg ggctccgcga tggaacgccc gaccgggtcg aagcgatcgg catcgggcgg
    23521 gcggtcgacg ccgcggagag cgcggacgtc gtcgtgttcg tgttggacgc ctcggagcgt
    23581 gcgctcgccg gcgatgtccg cgaggcactc gcgcgcctcg acgcgtcgcg cacgctcgtg
    23641 gtatggaaca aggtggatcg cggagaggac gtggacgccg gggctgcccc tcgcgatatt
    23701 gcacacgttc gcgcttcggc cgtgacgggt cagggcgtgg atgagatccg gcgagagctg
    23761 ctccgactgg cgggctcgga ggtgctggct cggactgcac gcgaccgggc tatcctcaat
    23821 gcgcgcctcg tcacgttgct cgcggacgct cgtgcccgac tggataccct gggccaagcg
    23881 gtcgagtcac gcgagccgct cgagctcctc gcggtccagg cgagagacgt attatcgcta
    23941 tatgaggagg cgacgggacg gcgctaccat gacggactcc tcgacgtgat cttctcgcgt
    24001 ttctgcatcg gaaagtgatt gtcaactttt ccgagcgccg attgtcactc tttcgcgccg
    24061 ataattgtca acgtaggatt gtcaacattc catggatcga cgcttcgaca tcatcgtaat
    24121 cggcggcggg catgcgggtt gtgaagcggc tctggcggcc gcgcgcatgg gcatgcatgc
    24181 gctcctatcg acgctccggc gcgatcggat cggccacatg ccgtgcaatc ctgccgtggg
    24241 cggtctggcg aaaggccacc tcgtcaagga gctagacgcg ctgggcggcg aaatcggtcg
    24301 ggccaccgac cgggccggca tccaattccg gatgctcaac cgcgcgaagg gcccggccgt
    24361 ctggtcgccg cgtgctcaag ttgacaaagt gatctacagc cggatcatgc gcgagacact
    24421 cgaggcccag cccggactca ccatcgtcga gggcgagatc gtcgatctga ttgtcaatca
    24481 gggcgcgttc gtgggcgttc ggttcgcaag cggcgagatt gtcaacgccg gcgcctgcgt
    24541 cctcacgacg ggcacgttcc tgcgggggct gatgcacacc ggcgaccgcc ggaccgacgg
    24601 cggccgcgag ggcgagccgc ccgccgcgac cctgtcggag agccttcgat cgctcggcct
    24661 tcgcatgggc cgtttgaaga ccggcacgcc gccgcgcgtt tttcgcgaca gcgtcgatct
    24721 ttcgcggatc gacccccaac ccggggacga cccgccggcc ccattctcct accgcaccgc
    24781 ccgcatcgag ctgccgcagg tgccgtgtca cctcacctac acgagcgagg ccacccacga
    24841 ggttattcgg cgcaatctcc atcggtcgcc gctctacggc ggtgccatcg agggcatcgg
    24901 cccgcgctac tgtccgtcga tcgaggacaa agtggttcgc ttcgcggaca aggatcgcca
    24961 ccaggtcttc atcgagccgg agggactcga ccatcccgag ctctacttga acgggatctc
    25021 caccagcttg ccggtcgacg tccagcttga gatcctgtcg tcgattccgg ggctggagtc
    25081 ggcgcggatg gcccggccgg gctatgcggt cgagtacgac ttcttcccgc ccgaccaact
    25141 ccaccgcacc atggagtcga aatgcatcga gcggctctac ttcgccgggc aggtcaacgg
    25201 cacgtcgggc tacgaggaag ccgcggcgca aggcttcctc gccggagtca atgcggtgct
    25261 gaagatccag ggcgaagcac ccttcgtccc gcgccgcaac gaagcctacc tcggcgtgct
    25321 ggtcgacgac ctcaccacca aggagatcga cgagccctac cgcatgttca cgagccgcgc
    25381 ggagtttcgc ctcgtgcttc gccaggacaa cgcggacgag cgcctgatgg cgaccgggca
    25441 ccgcttcgga ctcgtgcccg agcgcgcgct cgcaaccgtc cgcgagcgcg ttcgccgcgt
    25501 cgacgaggtt gtcgatgcgc tgggtcggcg gggctttccc gcgcgcgagg gtgcggcgcg
    25561 cttcgacgaa tggggcctcg aaccggttcg ccacggcacg acgctgcgcg acgtcctgcg
    25621 tcggccgggg gttcggctcg cacacttgcg cgatctcgcg ggcctggact gcgcgccctc
    25681 actcgacgac gcggtcgagt gccgcgtgaa gtacgagggc tacatcgacc gccaggcgcg
    25741 cgacgtccag gcgctgtccg acttggaagc gagacggatc ccgcccgact tcgcgttcga
    25801 agcactcgcg ggcctgagca ccgaggcgcg cgagaagctc cgcatcaagc ggcccgagac
    25861 gctgggccaa gcatcccgca ttgccggcgt gcgcgcgtcg gatctctcga ttctcgcggt
    25921 tcacgtcgag cgcgcgcgcc gaacccacgc cggatgagcg tacccgagaa cgacctcgag
    25981 accacactcg ccgacgtcgt tcggctgttt cccgctctct cgatcgatcc tcttcgacgc
    26041 tacacccgcg aaatcctgcg ctggaacgac gagatcgggc tggtctcgag gaaacacccg
    26101 gtcgcggcat gccaccgcct gctcatcgag agcgcggagt tcgggggcgt ggtggcgggc
    26161 gtacttgcgc ccgatcgatc gaccgacctg cgcgtcgccg acgtcgggtc gggcgccggc
    26221 ttccccggct tcgtgtggcg aatgctattt cccggctggg acctcgctct gatcgagcgc
    26281 cgcgagaaga aagccgtctt cctagagcag atcgtccgcc agttttcgtt ttcgcgcgtg
    26341 gaggtcttcg cgggcgacgc gcgcgctgca tcgcacctcg agcgctaccg cgcgtcgttc
    26401 gacgtcgtcg cgacgatggc ggtcggcgcg ccctcgcgga ccgcacccga gatcgaagac
    26461 ctcctcgttg ccggaggcgt cttc
//
LOCUS       DASPGK010000279         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1242113, whole genome shotgun sequence.
ACCESSION   DASPGK010000279 DASPGK010000000
VERSION     DASPGK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242113"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..643)
                     /locus_tag="VEC56_07670"
     CDS             complement(14..643)
                     /locus_tag="VEC56_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14069.1"
                     /translation="MNCNWAVTLVVGLSVVVAASRAACAERALAEWMYSASVYGYFVP
                     DDRDYVQPTISADRGVWHAELRYNYEDLETGSAWAGYTFAAGTELEFAITPMLGGVFG
                     RTVGVAPGFRCSIDYRALAFYSEGEHFLDAESDFDSFFYSWSELSVSPGGVIEVGAAA
                     QKTQAYESARDIHRGFLLGTSFESWGATVYVFNPDLPDPTYVLAAHADF"
     gene            complement(643..>3069)
                     /locus_tag="VEC56_07675"
     CDS             complement(643..>3069)
                     /locus_tag="VEC56_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV14070.1"
                     /translation="RFATLEPPVEGRVAGVIGADGIATLYTHQAEAIARARNGEDVVV
                     VTGTASGKTLCYNVPVLEAILADPSSCALYLYPTKALAQDQLRTLTRYLETDPSLPLV
                     AGTYDGDTPPNARRKLKNEANVLLTNPDMLHSGILPNHGTWARFLAKLKYVVVDEVHT
                     YRGTFGSNVAHVLGRLNRICEHYGAKPTFVCCSATIRNPRELAERLTGRSMQVVDDDG
                     SPRGPKAFVIWNPPFFGDDRVERKSANVEAARILAELVGFDHQAIAFVRARVVSEIVA
                     KYAQEFAAKISPSRAKLIKSYRGGYLPEERRQIERALFGGELKAVVSTNALELGIDIG
                     SMEAAVLVGYPGSIASTWQQAGRAGRGADPSLVVLLPYDSPIDQYMARHPDYFFGRSP
                     ENAVIDPLNAHIVLSHVRAAAFELPIRKEDWQQFGDFCGPILKLLEEDRQVRQQGGQF
                     YWSGPNYPSADVSLRNIGDDVYTIILGTSALMASEGLKAVPNETSRVIGTIDEAGAFQ
                     QLHPQAIYIHHGDTYFVNELDIRKKIAWVQKEDTDYYTQSITETKIKIEQEELHKSVQ
                     AAEVHWGDLSVTSVTYMFKKIKFGSRDSLGFGALDLPAQTLDTTGMWLLPHPSAYALA
                     KASGRVPREGLLGISNVIREVIPLYAMCDTMDVGASVDSSAANAPGIFVHDRFPGGLG
                     FALKAYELIDEILVACLELLDSCSCAHGCPSCVGSPLPPYSHLDPDVNARGLIPDKEA
                     AKSILHHLLGRDPFQPSVRTSAGSAEIARDEAELRKLVEATQLPGTLEKKLRQRVDKL
                     RRRGPTFGPR"
BASE COUNT          509 a         1000 c         1026 g          534 t
ORIGIN      
        1 gcggttccta cggtcagaaa tcggcgtgtg ccgcgagcac gtaggtgggg tcaggcaggt
       61 ccgggttgaa tacgtagacg gtcgctcccc acgattcgaa gctcgtcccg aggaggaacc
      121 cgcggtggat gtcgcgagca ctctcgtagg cctgcgtctt ctgtgccgcc gcgccgacct
      181 cgataacgcc cccgggcgac acactcagtt ccgaccacga atagaagaag gagtcgaagt
      241 cgctctcggc gtcgaggaag tgctcgccct cgctgtagaa cgcgagcgcc cgatagtcga
      301 tcgagcagcg gaagcccggt gcgacgccga cggttcggcc gaagaccccg cccagcatcg
      361 gcgtgatcgc gaactcgagc tccgttcccg cggcgaaggt gtagcccgcc caggccgacc
      421 cggtctccag gtcttcgtag ttgtagcgca gctccgcgtg ccacactccg cggtcggcgc
      481 tgatggtcgg ctgcacgtag tcgcgatcgt cgggcacgaa gtagccgtac acggacgcgg
      541 aatacatcca ctcggccagc gcgcgctccg cgcacgcggc ccgcgacgcg gcgacgacga
      601 cggacaggcc gacgacgagg gttacagccc agttgcagtt cactagcggg gtccgaaggt
      661 ggggccgcgg cgccgaagct tgtcgacccg ctgccggagc ttcttttcca gcgtgcctgg
      721 gagctgcgtc gcttcgacca gcttgcgcag ctcggcctcg tcgcgcgcga tttcggcgct
      781 cccggcgctc gtgcgcaccg agggttggaa gggatcgcgg cccagcaagt ggtgcaggat
      841 cgacttggcc gcttccttgt cgggaatcaa cccgcgcgcg ttgacgtcgg ggtcgaggtg
      901 cgagtacggc ggcagcggag agcccacgca cgacgggcag ccgtgcgcgc acgagcagga
      961 atcgaggagc tccaagcagg ccacgaggat ctcgtcaatc agctcgtacg ccttgagcgc
     1021 gaagcctagt ccgccgggga agcgatcgtg cacgaagatg ccgggcgcgt tggccgccga
     1081 cgaatccacc gacgcgccca cgtccatggt gtcgcacatc gcgtagagcg gaatcacctc
     1141 gcggatcacg ttcgatatcc cgagcaatcc ctcgcgcggc acgcggcccg aggccttcgc
     1201 gagcgcgtac gccgacgggt gcgggagcag ccacatgccg gtggtatcca acgtctgggc
     1261 ggggaggtcg agcgctccga aacccagcga gtcgcggctt ccgaacttga tcttcttgaa
     1321 catgtaggtc accgacgtca ccgacagatc gccccaatgc acctcggccg cctgcacgct
     1381 cttgtgcaat tcctcttgct cgattttgat cttggtctcg gtgatcgact gcgtgtagta
     1441 atcggtgtct tccttctgca cccacgcgat cttcttgcgg atatccaatt cgttgacgaa
     1501 gtaggtgtct ccgtgatgga tgtagatggc ctgcgggtgc agctgctgga acgcgcccgc
     1561 ctcgtcgatg gtgccgatga cgcggctggt ttcattgggc accgctttga ggccctcgga
     1621 cgccatcaat gcagacgtgc ccaggatgat ggtgtacacg tcgtcgccga tgttgcgcag
     1681 gctcacgtcg gcgctggggt aattgggacc gctccagtag aactggcctc cctgctggcg
     1741 gacctggcga tcctcttcga gcagcttcaa gatgggtccg cagaaatcgc cgaattgctg
     1801 ccagtcctcc ttgcgaatcg ggagctcgaa agcggccgcc cgcacgtgcg agagcacgat
     1861 gtgggcgttc aacggatcga tgaccgcgtt ctcgggcgag cgcccgaaga agtagtcggg
     1921 atggcgcgcc atgtactgat cgatgggcga atcgtacggc aataaaacga ccagcgatgg
     1981 atccgcgccg cgcccggcgc gcccggcctg ctgccacgtc gacgcgatcg agccgggata
     2041 gcccaccagc accgcggctt ccatcgaccc gatgtcgatc cccagctcga gcgcgttggt
     2101 gctgaccacc gctttcaatt cgccgccgaa gagcgcgcgc tcgatctggc gtcgctcctc
     2161 gggcagatac ccgccgcgat agctcttgat cagcttcgcg cgcgagggtg agattttcgc
     2221 cgcgaactcc tgcgcgtact tggcgacgat ctcgctgacc acccgcgcgc gcacgaacgc
     2281 gatggcctgg tggtcgaagc ccaccagttc ggccaagatg cgcgccgctt cgacgttggc
     2341 gctcttgcgc tccacgcggt cgtcgccgaa aaagggcggg ttccagatca cgaatgcctt
     2401 gggtccgcgc ggcgacccgt cgtcgtcgac cacctgcatc gaacgaccgg tcaggcgctc
     2461 cgccaactcg cgcgggttgc ggatggtcgc cgagcaacag acgaaggtcg gcttggcgcc
     2521 gtagtgctcg cagatgcgat tcaagcgacc cagcacgtgc gcgacgttcg acccgaaggt
     2581 cccgcggtac gtgtgcacct cgtccaccac gacgtacttc aacttggcga gaaagcgcgc
     2641 ccaggtcccg tggttgggca ggatccccga gtgcagcatg tcgggattgg tcaagagaac
     2701 gttggcttcg ttcttgagct tgcggcgcgc gttgggcggc gtgtcgccgt cgtaggtgcc
     2761 cgcgaccagc gggagcgacg ggtccgtttc caggtagcgc gtcagcgttc gcagctggtc
     2821 ctgggccagc gccttggtcg gatacaggta caacgcgcac gacgacgggt cggcgaggat
     2881 cgcttccaga accggcacgt tgtagcacag cgtctttccg ctcgcggtgc cggtgacgac
     2941 gacgacgtcc tcgccgttgc gcgcgcgcgc gatcgcttcc gcctggtggg tgtagagcgt
     3001 ggcgatcccg tcggcgccga tcacgcccgc cacgcgccct tcgacggggg gctcgagcgt
     3061 tgcgaagcg
//
LOCUS       DASPGK010000280         7780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1476988, whole genome shotgun sequence.
ACCESSION   DASPGK010000280 DASPGK010000000
VERSION     DASPGK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476988"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VEC56_07680"
     CDS             complement(<1..537)
                     /locus_tag="VEC56_07680"
                     /inference="COORDINATES: protein motif:HMM:NF027679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase family N-terminal domain-containing
                     protein"
                     /protein_id="HXV14071.1"
                     /translation="MNRKSLRVSVSLLALSVLLVASVWASAPVSPVLKGGTPVGDDVL
                     PYAPDRIIVKFKDTSMQLSTLKVPRQVGAVAPGTMTGLASVDALNQQMRVVQISRPFI
                     EPKNTAEARQIGLERTFMLHVANGVDIEDAVRRYSADPNVEYATPDWRAFPAVVPTDP
                     AYVQHWGHNNTAQLPGLDW"
     gene            complement(1042..1233)
                     /locus_tag="VEC56_07685"
     CDS             complement(1042..1233)
                     /locus_tag="VEC56_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXV14072.1"
                     /translation="MAEKLRRSRNKMIAGVCGGLAERFGWSATRMRVVYLLVSILSAA
                     FPGTIVYIVLWFIMPGPDD"
     gene            complement(1273..2955)
                     /locus_tag="VEC56_07690"
     CDS             complement(1273..2955)
                     /locus_tag="VEC56_07690"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXV14073.1"
                     /translation="MKTSFEISRDVPLVHMRQYMAQLGLAEDDFDFYGKYQGKIRLEA
                     LERWQDRPDGKLILVTAMTPTLRGEGKTLTSVGIGQAIGKLERRGMIALREPSLGPVF
                     GMKGGATGGGYAQVIPMEDINLHFNGDIHAITAAHNLLAAMLDNHIYQGNERRVDVTR
                     VLWPRALDMNDRALRHIVVGLGGHAHGIPRESGFIITAASEVMAILALATGREDLKKR
                     LGDIVIAYDQDYGAVTARDIGADRAMAVILKDAIQPNLVQTIEHTPALVHAGPFANIA
                     HGTSSVVAARTALKLADYVVTECGFGSDLGGEKFFDIVCRQSGLFPNAVVIVATARAI
                     KRHGGVSEKPPELLEKENMAAFDAGLVNLGAHVRGMQKFGVPIVVALNRFPFDTEAEI
                     AALRRFCDGLDVAFAPHDAFMRGGEGATDLARVAMELADSNPTPKPKFLYQLDEAPVE
                     KVRRVAREIYGASDVYFERDAATKLERFVKMGYGHLPVCIAKTQYSLSDNPKLIGAPR
                     DFTITVSDARLSAGAGFIVVVCGDMMLMPGLPVVPAAAKMDIDSSGTITGLF"
     gene            complement(3112..3390)
                     /locus_tag="VEC56_07695"
     CDS             complement(3112..3390)
                     /locus_tag="VEC56_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV14074.1"
                     /translation="MKKIYVGNLPFSATEEQVRTLFSKHGTVQSVSLINDRETGRPRG
                     FGFVEISDNGLAPALKALDGYEMDGRALKVNEAEAKPRNGGGGGGSRW"
     gene            3798..4181
                     /locus_tag="VEC56_07700"
     CDS             3798..4181
                     /locus_tag="VEC56_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXV14075.1"
                     /translation="MKRLPSLSRREREIMDVLYSAGSASAGEVHLRIPSPPSYSAVRA
                     TLRVLEQKGLITHEHDGKRYIYRPTVALNKARRSAVEHLLKTFFDGSAAGAVAALLEN
                     PGADLTEEDLDRMAALIERARKEGR"
     gene            4178..6667
                     /locus_tag="VEC56_07705"
     CDS             4178..6667
                     /locus_tag="VEC56_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXV14076.1"
                     /translation="MTMTPIFSSLLDSMTPESFAWWFSRASLLAAIACAFLAIVRSAR
                     PATRHAVAVTSLVAIALLPAASAILPTLSIPILPAPEAVVTPPAPRAMPFAIDAPAPR
                     ISSAPAVAPAPAVAPIPATAVTPAPVKPERPASRGPLAVIGRVALSSVRDSVASIGWK
                     GFAAMTWFMVTICLLGWTAMGAIGARRLSRRAASISDDELLDECERACRVLGIHRTVD
                     VGVSRDVAIPMVVGALRPRVVIPTSAAAWSRERLRIVLLHELAHVRRHDVAWLFVARV
                     VTSVFWFHPLAWVLSKSARRDAERACDEIVLASGVRGSDYAEHLVAIARTAMARDPLA
                     GSALAFATRSALEKRVVTILSARIPRATTSRRSFAASACAALALFALVAALRPTPVSS
                     ALVIPSASTDVVEAQHYSFDEKTTTKVEEKIDIDTAAPVLAGGTYYLAENDEEDRSGR
                     EWYARAKDYYDSRRFERSGEAYENAALRGYRRGTAFYNAGCSWALAGQKDKAMAALQK
                     SFDEGFDRPDLFAEDEDLNSLRGDARFQKLLSEVMSSGTAEAERRKATRDFERLAKAK
                     EVDEGDWNSVGIELLRSGDYDGAAKAFDNEFAVSKDEDALYNKACARSLAGKKEEALK
                     LLEQSITTGSVDADHMEQDPDLVPLHGQARFDQLLEMAEDLELHNAGWWKNLPGSSSW
                     SSGKDERRWRKSIPHFEEMTRKYPKVGRAWSNLGFAQLAAKDAEASTPSFQKALDLGY
                     RPSTTMYNLACSEAQSGNIDGAIAWLEKAEGAGFQMWSYARWDDDLDPIRDDPRYDKM
                     EKRWKAEAKKRGHDDWNWDHDHDKDYDKDDT"
     gene            complement(6767..7051)
                     /locus_tag="VEC56_07710"
     CDS             complement(6767..7051)
                     /locus_tag="VEC56_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV14077.1"
                     /translation="MKKIYVGNLPFTATEEQVRTLFGTHGNVMSVSLINDRETGRPRG
                     FGFVEISDEGLAPALKALDGFEMDGRALKVNEAENKPRNNGGGGMGGSRW"
     gene            complement(7350..>7780)
                     /locus_tag="VEC56_07715"
     CDS             complement(7350..>7780)
                     /locus_tag="VEC56_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXV14078.1"
                     /translation="IARQISLWDQRGSEVIRGNLLVIPIEESLIYVQPIYLRAEGGRI
                     PELKRVVVAYQNRVAMEETLETGLSRLFGGTVEAAAAAPAPPPPRVGDGGVADLSRRA
                     SEHYQRAIAAQRAGDWTRYGEELSRLGDVLRQLETTTGRE"
BASE COUNT         1311 a         2569 c         2582 g         1318 t
ORIGIN      
        1 ccagtcgaga ccgggcagct gggccgtgtt gttgtggccc cagtgctgca cgtaggccgg
       61 gtcggtcggc acgacggccg ggaacgcgcg ccagtccggg gtcgcgtact cgacgttggg
      121 atccgccgaa tagcggcgga ccgcgtcttc gatgtcgacc ccgttggcga cgtgcagcat
      181 gaacgtgcgc tcgagaccga tttgccgtgc ttcggcggtg ttcttcggct cgatgaaggg
      241 ccgcgaaatc tgaacgacgc gcatctgctg attgagcgcg tcgaccgatg cgaggcccgt
      301 catggtgccg ggtgccaccg cacccacttg gcgcgggacc ttgagcgtcg agagctgcat
      361 gctggtatcc ttgaacttga cgatgatgcg atcgggtgcg tacggcagga cgtcgtcacc
      421 gacgggcgtc ccacctttca ggaccggcga caccggagcg gatgcccaca ccgacgccac
      481 cagcaagacg gataatgcga gcaaagacac actaacgcgc aatgacttgc ggttcatctt
      541 tctctcctca catgttcgtt ttcatgtccc gcgacgagtc gtcgggcgac gatcgcacgg
      601 agacaccaag ggaaaagcga ccagggcgcg ttacgcccta tgtgggttgg atgatgtgtg
      661 cggatacctc cccgtcgcgg ccgcagcagg atcggtcgct cgctggtagc cggacgcacc
      721 gaccggggag acaagatggt cggtcggtga cgtcatccgc gagacgggcg gacgcgggga
      781 atcctagcga tccgggatag gcgcgtctac acaaatgtat gggaggccgt taacgtcccc
      841 cccgaatgaa ccgccggcgg ccgcgcggta gaatgattcc cgatccggta ctccgaaccc
      901 atgcggaaat cactcccggt cccgatctcc tggccatgct ggccggtggg ccgtccatta
      961 cgtgccggcg tcgtgtacaa cgcggcgtcg accgtgttcg gcgcgatggt cgtgttgcgg
     1021 taggcaaaga cccgtggccg cctaatcatc cggtcccggc atgatgaacc acagcacgat
     1081 gtacacgatg gttcccggga acgcggcgct caagatcgac accagcaggt agacgacgcg
     1141 catgcgggtg gccgaccacc cgaagcgttc cgcaagcccg ccgcacacgc ccgcgatcat
     1201 cttgttcctg gatctgcgga gtttctctgc caaccgaatc gcctcctcga tgcgcgcggc
     1261 ggtcgcgccg cgctagaaca agcccgtgat ggtgcccgac gagtcgatgt ccatcttggc
     1321 ggcggcgggg accacaggga gtcccggcat cagcatcatg tcgccgcaca ccaccacgat
     1381 gaagcccgca cccgccgaaa gccgcgcgtc gctcacggtg atcgtgaagt cgcgcggcgc
     1441 accgatgagc ttggggttgt cggacaggga atactgcgtc ttcgcgatgc acaccggcag
     1501 gtggccgtac cccatcttca cgaagcgctc gagcttggtg gcggcgtcgc gttcgaaata
     1561 gacgtcggac gcgccgtaga tctcgcgcgc gacgcggcgg accttctcca ccggcgcttc
     1621 gtcgagctgg tagaggaact tcggcttggg cgtcgggttc gagtcggcca attccatggc
     1681 cacgcgcgcc aggtccgtcg caccctcgcc gccgcgcatg aaggcatcgt ggggcgcgaa
     1741 ggccacgtcg agtccatcgc agaagcgccg cagcgccgcg atctccgcct cggtgtcgaa
     1801 cggaaaccga ttgagtgcca ccacgatggg tacgccgaac ttctgcatgc cgcgcacgtg
     1861 cgcgcccagg ttcacgaggc cggcgtcgaa ggcggccatg ttttcttttt cgaggagctc
     1921 gggcggcttt tccgacacac cgccgtggcg cttgatggcg cgcgcggtgg ccacgatcac
     1981 gaccgcgttg ggaaagagcc ccgactgccg gcacacgatg tcgaagaact tctcgccgcc
     2041 caggtcggaa ccgaagccgc actcggtgac cacgtagtcg gccagcttga gggccgtgcg
     2101 cgccgcgact acgctgctgg tcccgtgcgc gatgttcgcg aacgggcccg cgtgcaccag
     2161 cgccggcgtg tgctcgatgg tctgcacgag gttgggctgg atcgcgtcct tgaggatcac
     2221 ggccatggcg cggtcggcgc cgatgtcgcg cgccgtcaca gccccatagt cctggtcgta
     2281 cgcgatgacg atgtcgccca ggcgcttctt caagtcctcg cggccggtcg ccagcgccag
     2341 gatcgccatc acttcggacg cggccgtgat gatgaagccc gattcgcgcg ggatgccgtg
     2401 cgcgtgcccg cccaacccga cgacgatgtg gcgcaacgcg cggtcgttca tgtcgagcgc
     2461 gcgcggccac aggacgcgcg tcacgtcgac gcggcgctcg ttgccttggt agatgtggtt
     2521 gtcgagcatc gcggccagca ggttgtgcgc ggcggtgatg gcgtggatgt cgccgttgaa
     2581 gtggaggttg atgtcctcca tcggaatgac ctgcgcatac ccgccgcccg tcgcgccgcc
     2641 cttcatgccg aacacgggcc ccagcgacgg ctcgcgcagc gcgatcatgc cgcggcgctc
     2701 gagcttcccg atcgcctgcc cgatgcccac cgaggtcagc gtctttcctt caccgcggag
     2761 ggtcggcgtc atcgcggtaa ccaggatgag cttgccgtcc gggcgatcct gccagcgctc
     2821 gagcgcttcc aggcggatct tgccctggta ctttccgtag aagtcgaagt cgtcctcggc
     2881 gagcccgagc tgcgccatgt actggcgcat gtgcacgagg ggtacgtcgc gcgaaatctc
     2941 gaagctggtt ttcatcgtgg agtcctttgc agatgcggcg agtcgtacga cgatagcaga
     3001 gcgaaggatc cgtaacaacc ccgggcgaat cgaccgggcc caacgcacga agaccccgcc
     3061 atctttcgat gacggggtct ccgcatgact aacgcgtggg ttagacgagc cttaccagcg
     3121 gcttccgccg ccgccgccgc cgttgcgcgg cttggcttcc gcctcgttca ccttcagcgc
     3181 gcgcccgtcc atctcgtagc cgtcgagggc cttgagggcc ggggcgagcc cgttgtcgct
     3241 gatctccacg aagccgaagc cgcgcggacg accggtttcg cggtcgttga tcaagctgac
     3301 ggactgcacg gtgccgtgct tgctgaacag ggttctgacc tgctcttcgg tagcgctgaa
     3361 gggcaggttg cccacgtaaa tcttcttcat tcttctgttc tccaaaatgc cggatgaccg
     3421 gcttgtcgaa gcgcgcggaa tctccggcta gttccgggca tcccgcgagc gttcacgtgc
     3481 ggcccgacac ggtgccggac cgcaaatcca tcgtcgtcga ggggacctct aaggttgagc
     3541 gactctataa aagggaggct gaacgatatc gtcctgaagt cggggaggat ggcgtgcccg
     3601 aatcatacgg ccctacccgg gcggtgtaaa ggattaattt tgttaaagtt gggtcgcctg
     3661 gacccaccca accccgtgcc ccctcgccag aaagcccgtt gacggcccca cccaccatgt
     3721 gctagttttc cagcacttgc taaaaaacta gcaggtcaga ggcaactccc ccggaaagga
     3781 ctacgtacac caacgtcatg aaacgcctac ccagcttgag tcgccgcgaa cgggagatca
     3841 tggatgtcct gtacagcgcg ggcagtgcca gcgcgggcga ggtccatctg cggatcccct
     3901 ccccgcccag ttactcggcg gtccgcgcca cccttcgcgt cctcgaacaa aagggcctca
     3961 ttacgcacga gcacgacggc aagcgttaca tctaccggcc cacggtcgcc ttgaacaagg
     4021 cgcgccggag tgcggtggag cacttgctca agaccttctt cgacggttcg gcggcgggcg
     4081 cggtggccgc cctcctggaa aaccccggcg ccgatctgac cgaggaagac ctggatcgca
     4141 tggcggcgct catcgagcgc gcgcgcaagg aaggacgatg accatgaccc cgatcttttc
     4201 gtctttgctg gactcgatga cgcccgaatc gttcgcgtgg tggttctcgc gcgcgtctct
     4261 gttggcggcg atcgcgtgcg cgttcctcgc catcgtgcgc agcgcgcgcc cggcgacgcg
     4321 gcacgcggtc gcggtcacga gcctggtggc catcgcgctc ttgccggcgg cgtcggcgat
     4381 cctgcccacg ctctcgatcc ctattctgcc cgcgcccgaa gcggtcgtaa cgcccccggc
     4441 tccgcgcgcg atgccgtttg cgatcgacgc ccccgccccg cggatatcgt ctgctcccgc
     4501 ggtggcgccg gcgccggcgg tggcgccgat acccgcgacc gccgtcactc ccgcgccggt
     4561 gaagccggaa cggcccgcct cgcgcgggcc gctggccgtg atcgggcgcg tcgccctctc
     4621 gagcgtgcgc gactccgtcg cgtcgatcgg ctggaagggc ttcgccgcga tgacgtggtt
     4681 catggtgacg atctgcttgc tcggatggac tgcgatgggg gccatcggcg cgcggcgtct
     4741 ctcgcggcgc gcggcgtcga tatccgacga tgaactactc gacgaatgcg agcgcgcgtg
     4801 ccgcgtgctc gggatccatc gcacggtgga cgtgggcgtg tcgcgcgacg tggcgatccc
     4861 gatggtcgtg ggtgcgctcc gcccccgcgt cgtgatcccc acgtcggcgg ccgcctggtc
     4921 gcgcgagcgg cttcgcatcg tgttgctgca cgagctcgcg cacgtgcggc gccacgacgt
     4981 cgcgtggctg ttcgtcgcgc gcgtggtgac ctcggtattc tggttccacc cgctggcgtg
     5041 ggttctctcg aagagtgcac gtcgcgacgc cgagcgcgcg tgcgacgaga tcgtgctcgc
     5101 gagcggtgtg cgcggctccg actatgccga gcacctggtg gccatcgcgc gcaccgcgat
     5161 ggcgcgcgat cccctggccg gctcggcgct cgcctttgcg acgcgctcgg ccttggagaa
     5221 gcgggtcgtg acgattctgt ccgcgcgcat tccgcgcgcg actacctcgc ggcgttcgtt
     5281 cgcggcctcc gcttgcgcgg cgctggcact gttcgcgctg gtggccgccc tgcgaccgac
     5341 ccccgtgtcg tccgcgctcg tgattccgtc cgcatcgacc gacgtggtcg aagcgcagca
     5401 ctattcgttc gatgagaaga cgacgacgaa ggtcgaagag aagatcgaca tcgacaccgc
     5461 cgcacccgtg ctcgcgggcg ggacctacta cctcgccgaa aacgacgaag aggaccgctc
     5521 cggtcgcgag tggtacgcgc gcgcgaagga ctactacgac agccgccgct tcgagcgctc
     5581 gggcgaggcg tacgagaacg ccgcgctgcg cgggtaccgt cgcggtaccg cgttttacaa
     5641 cgcgggctgc tcgtgggcgc tcgcgggcca gaaggacaag gcgatggccg cgctccagaa
     5701 gtcgttcgac gagggcttcg accggcccga tttgttcgcc gaggacgaag acctgaattc
     5761 gctgcgcggc gacgcgcgct tccagaagct gttgagcgag gtcatgagct ccggcaccgc
     5821 ggaggccgag cgtcgcaagg cgacgcgcga cttcgagcgc ctcgccaagg ccaaggaggt
     5881 cgacgagggc gactggaact cggtcggcat cgaactcctg cgctccggcg actacgacgg
     5941 cgccgccaag gcgttcgaca acgagttcgc cgtcagcaag gacgaggacg cgctctacaa
     6001 caaggcgtgc gcgcggtcgc tggccggcaa gaaggaggag gcgttgaagc tcctcgaaca
     6061 atcgatcacc accggctccg tcgacgcgga tcacatggaa caggaccccg acttggtccc
     6121 gctccacggc caggcgcgct tcgaccagtt gctcgagatg gcggaggact tggaactcca
     6181 caacgccggc tggtggaaga atctccccgg cagttcgtcg tggagcagcg gcaaggacga
     6241 gcgccgctgg cgcaagtcga tcccgcactt cgaggaaatg acgcgcaagt accccaaggt
     6301 cgggcgcgcg tggtccaact tgggattcgc gcagctcgcg gccaaggacg ccgaggccag
     6361 cacgccgtcg ttccagaagg cactcgacct gggctacagg ccctcgacga cgatgtacaa
     6421 cctcgcctgc tccgaggcgc agtcgggcaa catcgacggc gcgatcgcat ggctcgagaa
     6481 ggccgagggc gccggcttcc agatgtggag ctacgcgcgc tgggacgacg acctcgatcc
     6541 gatacgcgac gacccgcgct acgacaagat ggagaagcgg tggaaggcgg aggcgaaaaa
     6601 gcgcgggcac gacgactgga actgggacca cgatcacgac aaggactacg acaaggacga
     6661 tacgtaggcg ctagctttcg ccgctttgga cgcgtccgcg cgaaagcaag aaggccccgc
     6721 caccctgcgg tgacggggcc tttgcgatgt caatcgtggg accagactac cagcggctgc
     6781 cgcccatgcc gccgccaccg ttattgcggg gcttgttctc cgcctcgttg accttcaggg
     6841 cgcgtccgtc catctcgaag ccgtcgaggg ccttcaaggc cggagcgagt ccttcgtcgc
     6901 tgatttccac gaagccgaag ccgcgcgggc gaccggtctc gcgatcgttg atcaaggaca
     6961 cggacatcac gttgccgtgt gtgccgaaca aggtccggac ctgttcttcg gtcgcggtga
     7021 agggcaggtt gcccacgtaa attttcttca tgagagtgtt ctccaaaatg ccggatcaac
     7081 cggcgtgtcg aagctctcgc gggatcgccg gaaaatcccg gtcacccgtt cgagcccaaa
     7141 gagtggtccg acaacgtgcc gggccgcgaa cgcatcaatc ggcgagggga cctaaaagtt
     7201 gagcgactct agaagaggga ggctgaacga tctcgtccgt gatcgaggag gatggtctgg
     7261 gagcatcatg cgcccacggc gcgagtatgt aaaggactat cttcgtttct ttcaagaggg
     7321 cgacgcgact tcgattcagg accgaatgat cactcgcggc ccgtcgtcgt ctccaactgc
     7381 cgcagcacgt cgcccagtcg cgacaattcc tcgccgtagc gcgtccagtc gcccgctcgc
     7441 tgcgcggcga tcgcgcgctg gtagtgctcc gaggcgcggc gcgagaggtc cgcgacaccg
     7501 ccgtcgccca cacgcggcgg cggaggtgcg ggggccgcgg ccgcggcttc gacggtgccg
     7561 ccgaacaagc gcgagagtcc cgtttcgagg gtctcttcca tggccacgcg attctggtac
     7621 gcgaccacca cgcgcttgag ctcggggatg cgcccccctt cggcgcgcag gtagatcggc
     7681 tgcacgtaga tcagcgactc ctcgatcggg atcaccagca aattgccacg aatcacctcg
     7741 gagccgcgct ggtcccacaa cgagatctgg cgcgcgattt
//
LOCUS       DASPGK010000281        13299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1126350, whole genome shotgun sequence.
ACCESSION   DASPGK010000281 DASPGK010000000
VERSION     DASPGK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126350"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="VEC56_07720"
     CDS             <1..1935
                     /locus_tag="VEC56_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXV14079.1"
                     /translation="AAPPQDGNLRATAKLSGTVGSAMIEALDVGIAALGATDPNVALT
                     ATGGPFDFTADSTQVFANARLKGTMNAGPLPLSGVTLVQKDPSKPVPVLGGEATARAN
                     FDGAMTAVAFDGEVDATNATYEVPAAMKKAAGIPAKATFRGTFRPQGTPHEGVEFESI
                     DVTLHSLRAKGSGTLVPFKGQEAMDLTFEGSTAIAPWKDLLPAMAPMSPSGDAKVSVR
                     VSGAPTPGAPPDIRGTATVTNFGAQIPNVPKPLSNGAATVAFTAKTARIPNATFAIGS
                     SKFKVDADVSSMTPMRATYTVSSPEVARLDVQAPAPNAKPLPRAEVFRDVVVKGEATE
                     KAPKLIENALTIASKSGIAANIDYTNAEAVVHATPEKSIIDRFSANAMGGTLSGSGIF
                     EPKTAKFDLSTKVEKVNLAEYFRFKSPLLADVLVGRISADFDIAGEGKTWEVLQKTLT
                     GSGGAVVVEGAFLNVNLAQQLFTSVQSMPMVPAGFADRIKNKNPKLFSANQTAFENLA
                     GKVTIADGRINTNDLHLKSSDFSLVGGGWFSFGKEMDVNTTLTLSQKLTNDLVAEVPM
                     AKYLLNSGGRFELPIKLSGAVAKPSVGVDANAIQAKLQQGLVQQGKDELNKKATDTVK
                     GLLDGLTKKKAPAPPDTTKK"
     gene            2084..3244
                     /gene="tgt"
                     /locus_tag="VEC56_07725"
     CDS             2084..3244
                     /gene="tgt"
                     /locus_tag="VEC56_07725"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXV14080.1"
                     /translation="MPFSFRVEARSQGARAGILVTDHGSIETPVFMPVGTQGSVKGVT
                     SRGVWDTGARLILGNTYHLYLRPGVDLIARAGGLHRFIGWDGALLTDSGGYQVFSLAS
                     LMRVTDSGVTFRSHIDGSLHEFTPEAVMEAQARIGADIAMSFDYFGGIPCERAEAERS
                     VALTTAWARRGLNATGARFDRYGYQQVVFGIVQGAEYADLRARSAQELTDLDFPGYAI
                     GGLSVGESKDATWDLAEGVTALLPQDRPRYLMGMGTPLDLIDAVARGVDMMDCVLPTR
                     NARNGTVFTRDGKVVLRNAAYAEDLAPLDSECACVACRNHSRAYLRHLFLSGEMLGPM
                     LASSHNLFFYADTMRAARGAIVRGEFDAWRSAFVDRFTRGDREAAQRAEVET"
     gene            3259..3576
                     /gene="yajC"
                     /locus_tag="VEC56_07730"
     CDS             3259..3576
                     /gene="yajC"
                     /locus_tag="VEC56_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXV14081.1"
                     /translation="MNSTLFAMMPADPSGAGPSPFSMLLPLLGMLAIFYFLLIRPQQK
                     RQKELRKMIDALKKGDRVVTASGLYGNVAGLRDDIVVVEIADGVKVEMLKNAVTAVVS
                     RSE"
     gene            3602..4120
                     /gene="def"
                     /locus_tag="VEC56_07735"
     CDS             3602..4120
                     /gene="def"
                     /locus_tag="VEC56_07735"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXV14082.1"
                     /translation="MKLQLRYYGDPILRKRASDVREFDHDLREFAESMIETMRREAGV
                     GLAATQVGVEKRVLVALQMENPEDSEAPEVVMVNPEILDRSPDTWIYEEGCLSIPGIR
                     GDVTRPEGIRVRYQDLHGKSHVVDVDGMFARVLQHEIDHLDGKLFIDYLSTGEKTLLK
                     TRLKKIAEKYGT"
     gene            complement(4194..4931)
                     /locus_tag="VEC56_07740"
     CDS             complement(4194..4931)
                     /locus_tag="VEC56_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HXV14083.1"
                     /translation="MATGLKDIGKWSLVVSAAMLMIMGACEDDEESPVAPPAPAPDKV
                     VQLDGNGDFISIPLANHTFNQFTLEVALKVPTYDSNVHYISQYQNAYLVLGDYSSGQI
                     STWANGLTPIDIGPATTTLSTDQWHHFAFSYDGTNQYALIDGVVVLSTPTTGTLTHNA
                     TTYNQALKIGARYSGDTQFVTGQLDEVRIWNIYRTPAEIQANMFSKISAQSGLVGYWN
                     FDDGTANDVTAYGADGTLNGDAVIVNQ"
     gene            5149..6081
                     /gene="fmt"
                     /locus_tag="VEC56_07745"
     CDS             5149..6081
                     /gene="fmt"
                     /locus_tag="VEC56_07745"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXV14084.1"
                     /translation="MHKLVFMGSPDFAVPSLDALVASKRFAPLLVVSQPDRPRGRGRE
                     ISPTPVRQRAIDRGIPSITMSKETYSKGVAAITNAKPDIVVVVAFGLILRRDLLDFPK
                     HGCINVHASLLPKYRGVSPIQAAILSGDAFTGCTTMHIDEGVDTGDVLLQEPTPILDT
                     DTAGSLTSRLAELGAALLVRTLDGLLDGSVTPQKQDHANATHTKKIRKEHGAIDWSRD
                     AAYLSRHARAMSPWPTAYTFHGSVRLIIESAAVGPKNPARPGTIVSLDPFAVATGDGT
                     LELHAVRPEGRRAMTAREFVAGYRLKVGDVFSQH"
     gene            6114..6839
                     /locus_tag="VEC56_07750"
     CDS             6114..6839
                     /locus_tag="VEC56_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HXV14085.1"
                     /translation="MDARRLAKRLLLLAGLFVAGVLVFNFVAMPMLVHQRGAVIVPDL
                     RNVSESQATESLARLGLKTKVGRSEYDPQVPKGFVVSHSPRANDSLKEGRTVTLITSL
                     GPRTERVPDVTKQTLRQARGVIEHAGLAVGRISRVKRAGEERDAVIATNPPAGEELRE
                     GEVVDLVVAVAGGGASYLMPDLTQQDLFFVREKLEKLGFRVSSVRYEAQEGVFPNTII
                     DQRPKPGARIREGESVELVASSN"
     gene            6859..7515
                     /gene="rpe"
                     /locus_tag="VEC56_07755"
     CDS             6859..7515
                     /gene="rpe"
                     /locus_tag="VEC56_07755"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXV14086.1"
                     /translation="MVAIAPSLLACDFSRLREEIRAAEEAGAEFLHLDVMDGHFVNNI
                     TFGPVLVKAIDKLATVPLDTHLMIAHPDRYIGAFLDAGADILTVHVEASSDPRRDLRT
                     IRARGKKAGLSLNPDTPLSAVEPYLEELDLLLVMSVVPGFGGQSFIDAVLPDLAKAAK
                     LREKLGLTFAIEIDGGINPETAARSRAAGTDILVAGTAIFRSPDYAAAIAAIRGGGTA
                     "
     gene            7606..8928
                     /gene="ffh"
                     /locus_tag="VEC56_07760"
     CDS             7606..8928
                     /gene="ffh"
                     /locus_tag="VEC56_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXV14087.1"
                     /translation="MFTSLTEKFERVFAAIRSRGKLSENDVRETVREVRKALLEADVN
                     FRVVKDFVARVEARLIGEEVHKSFTPEQQIIRVVNEELTQLLGGTAVPFHLKQATSTV
                     MVVGLQGSGKTTFSAKLAATLRKRGRKPVLVGLDVYRPAAMDQLEILGKQIAVPVSRG
                     QAGETDVIALLARGRDAARDRLCDTLILDTAGRLHIDDEMMNELVRLRDVAQPEEILL
                     VVDSMTGQEAVNVATAFHERLSLTGIVLSKLDGDARGGAALSVRSVVGVPITFASVGE
                     KLSDLEPFYPERMAGRILGMGDVLSLIEKTQETIDVDAAHELERRVRSQAFDLSDFLG
                     ELRRFKKMGPLEDIMKMIPGVSKALPAGAAVDGKQLGRVEAIICSMTPEERARPQLIN
                     GSRRKRIARGSGTTVQDVNQLLRQFEQMKKMMKTMGRMQKMKRKRLPF"
     gene            9066..9332
                     /gene="rpsP"
                     /locus_tag="VEC56_07765"
                     /pseudo
     CDS             9066..9332
                     /gene="rpsP"
                     /locus_tag="VEC56_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            9530..9760
                     /locus_tag="VEC56_07770"
     CDS             9530..9760
                     /locus_tag="VEC56_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXV14088.1"
                     /translation="MKALIEYVATSLVDDPKSVKVTPATKENYIVMELTVDPEDLGKV
                     IGKNGQTARAIRTLVNAAASHAGEKVIFEIKE"
     gene            9757..10278
                     /gene="rimM"
                     /locus_tag="VEC56_07775"
     CDS             9757..10278
                     /gene="rimM"
                     /locus_tag="VEC56_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXV14089.1"
                     /translation="MTDRIHLGRFVKAFGIKGELKLYASDDFWPEAFESEQLFIARET
                     DADNERSVVIEYGKPHGGQYIVKLEGVDDRNDAEEIVGEDLFIDGNEIDVPAPDKELP
                     HQVMGMTVRTDQGRVIGTITNVLVGPAQSVYEVTGEDGKVALIPAVPAFIVSRDDAKR
                     EIVIRVIPGLIDA"
     gene            10281..10964
                     /gene="trmD"
                     /locus_tag="VEC56_07780"
     CDS             10281..10964
                     /gene="trmD"
                     /locus_tag="VEC56_07780"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXV14090.1"
                     /translation="MRINVVTIFPETMESFFAAGMLGIAKKKGLVAFRCVNPREFTSD
                     AHRTVDDSPFGGGAGMVMMAPPIVDAVESLDRAPGSPVVLMGPAGKRFEQSDAERLSH
                     QKEITFVCGRYKGVDERVRDLVVTEEISLGDFVLSGGEVAAAAVIEAVVRLVGGVLGD
                     DESAHTDSFAAGREGKLDCAWYTRPAEYRGLKVPDVLMSGHHAEIEKWRRASSLERTR
                     RLRPDLLKE"
     gene            11092..11445
                     /gene="rplS"
                     /locus_tag="VEC56_07785"
     CDS             11092..11445
                     /gene="rplS"
                     /locus_tag="VEC56_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXV14091.1"
                     /translation="MNIMDRIDQRSLSKKEPDFRVGDTVKVMVRVVEGNKEREQAFQG
                     VVIKQSGRGVAETFTVRKVSSGVGVERVFPIHSPNVKSIQVVRRGRVRRAKLYYLRKR
                     VGKAARIQEKHDKGE"
     gene            11399..12151
                     /locus_tag="VEC56_07790"
     CDS             11399..12151
                     /locus_tag="VEC56_07790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXV14092.1"
                     /translation="MARRPASKKSTIRASKRPGRAAKSAGRKKATNDGAAAEDALRAR
                     FRSLVEFDRELTSRGYASIAGVDEAGRGALAGPVVAAAVVLREDCNLIRIYDCKAVAE
                     ADRESLFLDIVASAVSVGIAFSHPAVIDRDNILRATLASMHRAVEALRLTPDIVLIDG
                     REGIQWPGRVVPVIKGDAKSLCVAAASIVAKVARDRFMRRLHRAFPSYHFDQNKGYGT
                     RDHLAAIAAFGAAAVHRRSFLGKVVANEPSMF"
     gene            12206..12559
                     /locus_tag="VEC56_07795"
     CDS             12206..12559
                     /locus_tag="VEC56_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXV14093.1"
                     /translation="MNTRASGELGERIAAAFLILKGYRILDANVRVARREVDLVATDG
                     HAIIAVEVKLRWGRRYGAAAEAIDERKLARLRVALSGIARELGDRGSPRIDVVTIDVD
                     ETGDRMAVEHYVGVT"
     gene            12629..13228
                     /locus_tag="VEC56_07800"
     CDS             12629..13228
                     /locus_tag="VEC56_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14094.1"
                     /translation="MRKFMPIVVVAVAAAACSSVKEVATQDMPLEVQHQILDEYKDRY
                     VWTRVQVQDLGEGGVIPRDEKVKIVDVGMFKAGSVTVETLKKKNRVVQGLSLDKPLTK
                     AKIDSALTEYFWFDDPTLRHVAFIRKFGKDTAKLIMNHDLVAGMSPEAALASWGKPAK
                     TERSERNGVVQERWRYPTAQVGRTKDILFENGKVRSWDQ"
BASE COUNT         2464 a         4163 c         4493 g         2179 t
ORIGIN      
        1 gccgcgccgc cgcaagacgg caatcttcgc gcgaccgcga agctctcggg gacggtcggc
       61 tctgccatga tcgaggcgct cgacgtcggc atcgcggcgt tgggcgcgac cgatcccaac
      121 gtcgcgctca ccgccaccgg cgggcccttc gacttcaccg cggattcgac ccaggtgttc
      181 gcgaacgcgc gcctcaaagg cacgatgaac gcgggaccgc tgccgctctc gggtgtgacg
      241 ctcgtgcaga aagacccgtc gaagccggtg cccgtgctgg gcggtgaggc caccgcgcgc
      301 gcgaacttcg acggggccat gaccgcggtg gcgttcgacg gcgaggtgga cgcgaccaac
      361 gcgacctacg aagtccccgc ggccatgaag aaggcggccg gcattcccgc caaggcgacc
      421 ttccgcggga cgtttcgccc gcaggggacg ccccacgagg gcgtcgagtt cgagtccatc
      481 gacgtcacgc tgcactcgct gcgcgcgaag ggctccggca cgctggtgcc gttcaagggc
      541 caggaggcga tggatctcac gttcgaggga tcgaccgcga tcgcgccgtg gaaggacctc
      601 ttgcccgcga tggcgccgat gtcgccctcc ggcgacgcca aggtgagcgt gcgcgtgagt
      661 ggtgccccta cgcccggcgc gccacccgac attcgcggca ccgcgaccgt gaccaacttc
      721 ggcgcgcaga ttcccaacgt gcccaaaccg ttgagcaacg gcgcggcgac cgtcgcgttc
      781 accgccaaga ccgcgcgcat ccccaacgcg accttcgcga tcgggagcag caaattcaaa
      841 gtagacgctg acgtgtcgtc gatgacaccc atgcgggcga cctacacggt gtcctcgccc
      901 gaggtcgcgc gcctcgacgt acaggcaccc gcgcccaacg ccaagccgct cccgcgcgcg
      961 gaggtctttc gcgacgtcgt ggtcaagggc gaagccacgg agaaggcgcc caagctgatc
     1021 gagaacgcgc tcaccatcgc gtcgaagagt gggatcgcgg ccaacatcga ttacaccaac
     1081 gcggaggcgg tggtgcacgc cacacccgag aagtcgatca tcgatcgctt ctcggccaac
     1141 gcgatgggcg ggacgctttc cggatcgggc atcttcgagc ccaagacggc caagttcgat
     1201 ctctcgacca aagtggagaa ggtgaacctc gcggagtact tccgcttcaa atcgccgctg
     1261 ctcgccgatg tattggtcgg acgcatcagc gcggacttcg acatcgcggg cgagggcaag
     1321 acgtgggagg tgctccagaa gacgctgact gggtcgggcg gggcggtcgt ggtcgagggc
     1381 gcctttctca atgtgaacct cgcgcagcag ttgttcacct cggtgcagtc gatgccgatg
     1441 gtaccggccg gcttcgccga ccgtatcaag aacaagaatc cgaagttgtt ctccgccaac
     1501 cagactgcgt tcgagaatct cgcgggcaag gtgacgatcg ccgacggccg catcaacacc
     1561 aacgacttgc acctcaagtc gtcggacttc tcgctggtgg gcggcgggtg gttctcgttc
     1621 ggcaaggaga tggacgtcaa cacgacgctc acactctcgc agaagctcac caacgacttg
     1681 gtcgcggaag tcccgatggc gaagtacctg ctgaactcgg gcgggcgctt cgagttgccg
     1741 atcaagctct cgggtgcggt ggcgaagccg tcggtcggtg tggatgccaa cgcgattcaa
     1801 gcgaagctgc agcagggcct ggtccagcaa ggcaaggacg agctcaacaa gaaggcgacc
     1861 gacacggtca agggcttgct cgatggccta acgaagaaga aggcgcccgc gccgcccgac
     1921 accaccaaga agtagcgcac ggcggccgat gcgatcgtcc ctctccgctc aaactgcagc
     1981 gcgagacgga cagtatcgtg ttgtattcgc gctgcagaaa tcgcgttcga gggatcgatc
     2041 gcatcggctg gcgtcggcga cgcctgcgtg gtaaagtaac cccatgccgt tttcgtttcg
     2101 agtcgaggcg cgctcgcagg gtgcgcgcgc ggggatcctt gtcaccgatc atgggtcgat
     2161 cgaaaccccg gtgttcatgc cggtggggac acaggggtcc gtcaagggtg tcacgagccg
     2221 cggcgtgtgg gacaccggag cgcggctcat cctcggcaat acgtatcact tgtaccttcg
     2281 cccgggcgtg gatctaattg cgcgcgcggg gggattgcat cgcttcatcg gctgggacgg
     2341 ggcactcttg accgacagcg gcggctacca agtgttcagc ctggcctcac tcatgcgcgt
     2401 gacggattcg ggcgtcacct ttcggtcgca catcgacggg tcgctgcacg aattcacgcc
     2461 cgaagcggtg atggaggcgc aggcgcgcat cggcgccgac atcgcgatga gcttcgacta
     2521 tttcggcggg attccgtgcg agcgcgcgga agccgaacga tcggtggcac tcacgaccgc
     2581 gtgggcgcgg cggggtttga acgcgaccgg cgcgcgcttc gatcgctacg gctaccaaca
     2641 ggtggtgttc gggatcgtgc agggcgcgga atacgcggat ttgcgcgcgc gctcggcgca
     2701 agaattgacc gacctcgact ttcccggtta cgccatcggc ggactgtcgg tgggcgagtc
     2761 gaaggatgcg acctgggacc tcgcggaagg tgtcacggcc ttgttgccgc aagaccgccc
     2821 gcgctacttg atgggcatgg ggacgccgct ggacttgatc gacgccgtcg cgcgcggggt
     2881 cgacatgatg gactgcgtgc tgcccacgcg taacgcgcgc aacgggaccg tgttcacacg
     2941 cgacggcaag gtggtgctgc gcaacgcggc ctacgcggaa gacctggcac cgctcgatag
     3001 cgagtgtgcg tgcgtggcgt gccggaatca ttcgcgcgcg tatttgcggc acttgtttct
     3061 ctcgggcgag atgctgggac ctatgctggc cagctcgcac aacttgttct tttatgccga
     3121 caccatgcgc gccgcccgcg gcgccatcgt gcgcggcgaa ttcgacgcct ggcgtagcgc
     3181 cttcgtcgac cgattcacgc gcggcgaccg cgaggccgcc cagcgcgcgg aagtagaaac
     3241 ctaaccaagg aggacccttt gaattccacg ttgttcgcga tgatgcccgc cgaccccagc
     3301 ggtgcgggcc ccagtccgtt ctcgatgctg ctcccgctcc tcgggatgct cgcgattttc
     3361 tacttcctgt tgatccggcc ccagcagaag cgccagaaag agctgcgcaa gatgatcgac
     3421 gccttgaaga agggcgaccg tgtggtcacc gcgagcgggc tgtacgggaa cgtcgcgggg
     3481 ctgcgcgacg acatcgtggt ggtcgagatc gccgacggcg tcaaggtcga gatgctcaag
     3541 aacgccgtga ccgcggtggt gtcgcgctcg gagtagaacc gacggtttct gcttaagagg
     3601 gatgaaactg caacttcgat actacggcga tcccatcctg cgcaagcgcg cctccgacgt
     3661 gcgcgagttc gaccatgacc tgcgcgaatt cgcggagagc atgatcgaaa ccatgcgccg
     3721 cgaggccggc gtgggcctgg ccgccacaca agtgggagtc gagaagcgcg tcctggtggc
     3781 actccagatg gagaaccccg aggacagcga ggcccctgag gtcgtgatgg tcaatcccga
     3841 gatcctcgac cgctcgcccg acacgtggat ctacgaggag ggatgcttga gcatccccgg
     3901 catccgcggc gacgtgaccc ggccggaggg catccgcgtc cgctatcaag acctgcacgg
     3961 caagagccac gtggtcgacg tcgacggcat gttcgcgcgc gtcctccagc acgagatcga
     4021 ccacctggac ggcaagctat tcatcgacta tctctcgacg ggagagaaga cgctgctcaa
     4081 gacgcgcctg aagaagatcg cggaaaagta cgggacttga gtcaaccggg gccgatacgc
     4141 cgcgaagccc gacccgcccc aaaggcgggt cgggcttcgg cttgtccaag cgcctattga
     4201 ttgacgatga cagcgtcgcc gttaagcgtt ccgtcggcgc cgtaggcggt tacgtcgttg
     4261 gcggtgccgt cgtcgaagtt ccagtacccg acgagcccgc tctgcgcaga gatcttcgag
     4321 aacatattgg cttggatctc ggccggcgtg cgatagatgt tccagatgcg aacctcgtcg
     4381 agttgacccg ttacgaactg agtgtccccg gagtaccggg cgccgatctt cagcgcttga
     4441 ttgtacgtcg tggcattgtg ggtgagcgtg ccggtcgtcg gcgtcgacag aacgacgacg
     4501 ccgtcgatga gcgcgtactg gttggtgccg tcatagctga aggcgaagtg gtgccactga
     4561 tccgtgctca gggtggtcgt ggccgggccg atgtcgatgg gagtcagtcc gttcgcccat
     4621 gtggatattt gcccggaact gtaatcgccc agcacgaggt aggcgttctg atactggctg
     4681 atgtagtgga cgttgctatc gtacgtgggg accttgagcg ccacctccag ggtgaattgg
     4741 ttgaatgtgt gattcgccag cggaatggag atgaaatctc cgttcccgtc tagctgaacc
     4801 accttgtcgg gagccggcgc cggcggtgcg acggggcttt cttcgtcgtc ttcgcacgca
     4861 cccataatca tcagcatggc ggcactcaca acgagcgacc acttcccaat gtcttttaga
     4921 cccgtagcca tcgtttggac ctcccaatga aggatacgat tccaccccga gccttccgtg
     4981 caccgcggtg gcttttgcgt gataggactg tacctgtccg cgcatggcaa gaactatttg
     5041 acccacgacg gcgattgacc ccggccccca gcgaggaata cgctagccgc gtggggcgtc
     5101 ccaaccgcga tatgtcgagc ccggcggggg gctagcgtgc ccgagccgat gcacaagctc
     5161 gtcttcatgg gaagccccga cttcgcggtg ccgtcgttgg acgcgctggt cgcgtcgaag
     5221 cgtttcgcgc cgctcttggt cgtgagccag cccgaccgcc cgcgcgggcg cggcagagaa
     5281 atctccccca cgccggttcg acagcgcgcc atcgaccggg gtattccgtc gattacgatg
     5341 tcgaaggaga cctactccaa gggtgtggcc gcgatcacca atgccaagcc agacatcgtc
     5401 gtggtggtcg cgttcggttt gattctccgg cgtgatctgc tcgatttccc gaagcacggc
     5461 tgcatcaacg tgcacgcctc gctgctcccg aagtaccgcg gcgtctcgcc catccaagcg
     5521 gcgattctct cgggcgatgc gttcacgggt tgcacgacga tgcacatcga cgaaggggtg
     5581 gacacgggcg acgtgctgtt gcaagagccg acgccgatcc tggacacgga cacggcgggg
     5641 tcgctcacgt cgcgtctcgc ggagctggga gcggcgctct tggtgcggac cctcgacgga
     5701 ttgctcgacg gcagcgtgac gccccagaag caggatcacg cgaacgcgac ccacaccaag
     5761 aagattcgca aggagcacgg cgccatcgac tggtcgcgcg acgcggccta cttgtcgcgc
     5821 cacgcgcgcg cgatgtcgcc gtggccgacc gcgtatacct tccacggcag cgtacgactc
     5881 atcatcgagt cggccgccgt gggccccaag aaccccgcga gacccggcac catcgtctcg
     5941 ctcgacccgt tcgcggtcgc gacgggagac ggaaccctcg agctccacgc ggttcgcccg
     6001 gaaggccggc gcgccatgac ggcgcgcgag ttcgtggcgg gctatcgact caaggtgggc
     6061 gacgttttct cccaacattg acgaatccgc gcgcgaaggg ctacagtcgc gccgtggatg
     6121 cccgtcgcct cgccaaacgc ctcctcttgc tggccggatt gttcgtcgcc ggcgtactcg
     6181 tgttcaactt cgtcgccatg ccgatgctgg tccaccagcg cggggccgtg atcgtgccgg
     6241 atcttcgcaa cgtgagcgag tcgcaagcca ccgagtccct ggcgcgcctg ggactcaaga
     6301 ccaaggtggg gcgcagcgag tacgacccgc aggtgccgaa gggcttcgtc gtgtcgcact
     6361 cgccgcgcgc gaacgacagc ttgaaggaag ggcgcacggt gacgctgatc acgagcctgg
     6421 gcccgcgcac cgagcgcgtc cccgacgtga cgaagcaaac gttgcgacag gcgcgcggag
     6481 tgatcgagca cgcgggcctc gcggtggggc gcatctcgcg cgtcaagcgc gcgggcgaag
     6541 agcgcgacgc ggtcatcgcc accaatccgc ccgcgggcga agaattgcgc gaaggcgaag
     6601 tggtcgattt ggtggtcgcg gtcgcgggcg gcggtgccag ctacctcatg cccgatctca
     6661 cccagcagga cttgttcttc gtgcgcgaga agctggagaa gctgggcttt cgggtgtcgt
     6721 cggttcgcta cgaggcgcag gaaggcgtgt ttccgaacac catcatcgac cagcgcccca
     6781 agccgggcgc tcgcatacga gaaggggaat cggttgagct ggtcgcatct agtaactagc
     6841 gcgacgaagt cgcgcaacat cgtcgccatc gctccaagtc ttctagcgtg tgacttttcc
     6901 cgtctaagag aggagattcg tgcggcagaa gaggccggag cagagttcct ccacctcgat
     6961 gtgatggacg gtcacttcgt caataacatc accttcggcc cggtcttggt gaaggcgatc
     7021 gacaaactcg ccaccgtccc gctcgacacg cacttgatga tcgcgcaccc cgaccgctac
     7081 atcggcgcct tcctcgacgc cggcgccgac attctcaccg tccacgtcga ggcgagcagc
     7141 gacccccgcc gcgacctgcg caccatccgc gcccgcggca agaaggcggg cctctcgctc
     7201 aacccggaca cgcccctttc ggcggtcgag ccctacttgg aagagctgga cctcctcttg
     7261 gtgatgagcg tggtgcccgg cttcggcggc cagagcttca tcgacgcggt cctccccgac
     7321 ctcgccaagg cagccaagct ccgtgagaaa cttggactta ccttcgcgat cgagatcgac
     7381 gggggcatca atcccgaaac agccgcgcgc tcgcgcgcgg ccgggacgga catcctggtg
     7441 gccggaactg ccattttccg aagccccgac tacgcggcgg ccatcgccgc gatccgcggc
     7501 ggcgggacgg cgtaacccgg cctcccaaat aggacttgcc cgccgctcca ggggtctgct
     7561 accctcctcc gtccggtcga agcccgtccg ctccccgcgc aattcatgtt cacgagcctg
     7621 accgagaagt tcgaacgcgt gttcgcggcc attcgctcgc gcggcaagtt gagcgagaac
     7681 gacgtgcgcg agaccgttcg cgaggtgcgc aaggcactcc tcgaagcgga cgtcaacttc
     7741 cgcgtggtca aggacttcgt ggcgcgcgtg gaagcgcgct tgatcggcga agaagtccac
     7801 aagagcttca cccccgagca gcagatcatt cgggtggtga acgaggagct gacccaactc
     7861 ctcggcggga cggcagtacc gtttcacttg aagcaggcga cctcgaccgt catggtggtc
     7921 ggcctgcagg gatcgggcaa gacgacgttt tcggccaagc tcgcggcgac gctgcgcaag
     7981 cgcggccgca agcccgtgct ggtgggactc gacgtctatc gccccgcggc gatggaccag
     8041 ctcgagattc tcggaaagca gatcgcggtt ccggtttcgc gcgggcaggc cggcgagacc
     8101 gacgtgatcg cgctccttgc gcgcggccgt gacgcggccc gcgaccggct gtgcgacacg
     8161 ctgattctcg acaccgcggg gcggctccac atcgacgacg agatgatgaa cgagctcgtt
     8221 cgtctgcgcg atgtggccca gcccgaagaa atcctgctcg ttgtggacag catgacgggg
     8281 caggaagcgg tcaacgtcgc gaccgcgttc cacgagcgac tgtcgctcac cggtatcgtt
     8341 ctctcgaagc tcgacggcga cgcgcgcggc ggtgcggccc tttcggtgcg gtccgtagtc
     8401 ggggtgccga tcacgttcgc gagcgtcggc gagaagctct cggacctgga gccgttctac
     8461 cccgagcgca tggcggggcg gattctcggg atgggcgacg tgctgtcgct catcgagaag
     8521 acgcaggaaa ccatcgacgt cgatgcggcg cacgagctcg agcgccgcgt gcgctcgcag
     8581 gccttcgacc tgtcggactt cttgggagag ctgcggcgct tcaagaagat gggcccgctc
     8641 gaggacatca tgaagatgat cccgggtgtg agcaaggcgc tgcccgcggg cgccgccgtc
     8701 gacggcaagc agttggggcg cgtcgaggcc atcatctgct cgatgacgcc cgaagagcgc
     8761 gcgcgaccgc agttgatcaa cgggagccgc aggaagcgca tcgcgcgcgg cagcggcacc
     8821 accgtgcagg acgtcaatca gctgctgcgc cagttcgagc agatgaagaa gatgatgaaa
     8881 accatgggtc gcatgcagaa gatgaaacgc aagcggctgc cattctagga aggcctcggg
     8941 tggcgtccaa cgggcgccca caagcgaggg actactagtg cgcaggcgcg gtaggcccga
     9001 gcgtagtggg ctgttggacg acaggacccg aggccatcgc agtcaactcg tcggaggtta
     9061 gaagagtgtc cgtcaaaatt agattgaaac gcatgggaac caagaagcgc ccgttctatc
     9121 gcatcgtggc gaccgattcg cgcaatcgcc gcgacggccg cttcatcgaa gagctgggct
     9181 attacgatcc gttgacgagc ccgcccaaca tcaagatcga cgagccgttg atcatgaagt
     9241 ggatgggcga cggggccatt ccgtcggaga acgtcgaagg cttgctgcgc cgcaccggca
     9301 ccatgaagaa gtgggtgctg gtcaagcagg gcgtgcccct cgaccagatc gacgccaagc
     9361 tcgcgaccct gacggacgcc gagaccaagg gtctctcggc cgacgagcgc cgcaagaagt
     9421 tcacgagccg gaagctctcc aagaaggcgg ccgccaaggc caagggcgcc gcggccgaga
     9481 gcgcgtagtt cttcggagtc ccgacgtggc gcaagcgagc acggagtcca tcaaggcgtt
     9541 gatcgagtac gtcgcgacct cgctggtcga cgaccccaag tcggtcaagg tgacgccggc
     9601 caccaaggag aactacatcg tgatggaatt gaccgtggac cccgaggacc tgggtaaggt
     9661 gatcgggaag aacggccaga ccgcgcgcgc gattcgcacc ctggtcaacg cggccgccag
     9721 ccacgcgggc gagaaagtga tcttcgagat caaagaatga cggaccggat ccatctcggg
     9781 cgtttcgtca aggcgttcgg catcaaaggt gaactgaagc tctacgcctc cgacgacttc
     9841 tggcccgagg cgttcgaatc ggagcagctg ttcatcgcgc gcgagacgga cgcggacaac
     9901 gagcgctcgg tcgttatcga gtatggaaag ccgcacggcg gccagtacat cgtgaagctc
     9961 gagggcgtgg acgatcgcaa cgacgccgaa gagatcgtgg gcgaggatct gttcatcgac
    10021 ggcaatgaaa tcgacgtgcc ggcacccgac aaggagctgc cgcaccaggt gatgggaatg
    10081 acggtccgca ccgaccaggg acgcgtcatc ggaaccatca cgaatgtgct ggtcgggccc
    10141 gcgcagagcg tgtacgaagt gacgggcgag gacggcaaag tggcgctgat cccggccgta
    10201 ccggccttca tcgtctcgcg cgacgacgcc aaacgagaga tcgtgattcg cgtgattccg
    10261 ggactgatcg acgcgtgacg atgcgcatca acgtcgtcac catattcccg gagacgatgg
    10321 agtcgttctt cgcggcggga atgctcggga tcgcgaagaa gaaggggctg gtcgcgtttc
    10381 ggtgcgtgaa cccgcgcgag ttcacgagcg acgcgcaccg caccgtggac gattcgccct
    10441 tcggcggcgg ggccggcatg gtgatgatgg ccccacccat cgtggacgcg gtcgagagcc
    10501 tcgaccgcgc gccgggaagc ccggtggtgc tgatgggccc ggcgggcaag cgcttcgagc
    10561 agagtgacgc cgagcgcctg tcgcaccaga aggagatcac cttcgtgtgc gggcgctaca
    10621 aaggcgtgga cgagcgcgtg cgcgacttgg tagtcaccga agagatatcg ctcggcgatt
    10681 tcgtgctgag cgggggcgag gtggcggcgg cggcggtgat cgaggcggtg gtgcggttgg
    10741 tgggcggtgt tctgggcgac gacgaatcgg cccacaccga ttcgttcgct gccggacgcg
    10801 aaggaaaact cgactgcgcg tggtacacgc ggcccgccga atatcgcggg ctcaaagtcc
    10861 ccgacgtctt gatgtcgggg caccacgccg agatcgaaaa atggcggcgc gcgtcgtcgc
    10921 tcgagcgaac ccggcgtctt cggccggatt tgctgaagga atagagacga agagggtctc
    10981 gatgcgtccc ccggcgggtg gcgcggctgg ggcccccgcg gcgtaagtcc gcggggtgag
    11041 acgcgacagg acccgccggg cagcgtcagt tcggaacaag gaggaagtgc tatgaacatc
    11101 atggaccgga tcgaccagcg gagtctctcc aagaaggagc ccgatttccg cgtgggcgat
    11161 accgtcaagg tcatggtgcg cgtcgtcgaa ggcaacaagg agcgcgagca ggcgttccag
    11221 ggtgtcgtca tcaagcagag cggccgcggt gtggccgaga cgttcaccgt acgcaaggtc
    11281 agctccggcg tgggtgtgga gcgcgtgttc cccatccact cgcccaacgt taagtcgatc
    11341 caagtggttc gtcgcgggcg tgtgcgccgc gcgaagctct actacttgag gaagcgcgtt
    11401 ggcaaggcgg cccgcatcca agaaaagcac gataagggcg agtaagcggc ccgggcgcgc
    11461 agcaaagtcg gcgggtcgca agaaggcgac caacgacgga gccgcggccg aggacgcact
    11521 gcgcgcgcgc tttcgctcgc tggtcgagtt cgaccgcgag ctcacctcgc gcggctacgc
    11581 ctccatcgcc ggcgtggacg aagccggccg cggcgccctg gccggtccgg tggtggcggc
    11641 cgcggtggtg ctgcgcgagg actgcaacct cattcgcatc tacgactgca aggcggtcgc
    11701 cgaagccgac cgcgaatcgt tattcctaga catcgtcgcc tccgccgtgt cggtcgggat
    11761 cgcgttttcc caccctgccg tgatcgatcg cgacaacatc ctgcgcgcga ccctggcgtc
    11821 gatgcaccgc gcggtggagg cactccggct cacccccgac atcgtgctga tcgacggccg
    11881 cgagggcatc cagtggcccg ggcgcgtggt gcccgtgatc aagggcgacg ccaagagcct
    11941 gtgcgtggcg gccgcctcga tcgtggccaa agtcgcgcgc gaccgcttca tgcgccgcct
    12001 gcaccgcgcc ttcccgagct atcacttcga ccagaacaag gggtacggga cgcgcgacca
    12061 cctggcggcc atcgcggcct tcggcgcggc ggccgtgcac cgacggtcgt tcctgggaaa
    12121 agtggttgca aacgagccgt cgatgttcta atgattacag ggggttgcgg ggtttttggg
    12181 ctcggggttt gggtggaggt ggaggatgaa cacgagagcc agcggggaac tgggtgagcg
    12241 gatcgcggcc gcgtttctca ttttaaaggg gtatcgaatc ctggacgcga acgtacgcgt
    12301 cgcgcgccgc gaggtggatc tcgtcgcgac cgacggccac gcgatcatcg cggtggaagt
    12361 gaagctccgc tgggggcgtc gctacggagc cgcggcggag gcgatcgacg aacgcaagct
    12421 cgcgcggttg cgggtcgcgt tgtcgggaat cgcccgcgag ctgggagaca gaggctctcc
    12481 gcgcatcgat gtggtgacca tcgacgtcga cgaaaccggc gaccgcatgg cggtcgagca
    12541 ctacgtcggc gtcacctagg aatcgagaat gccccacgcg tcgcgagccg gtaccggctc
    12601 gcaaaaaatc gcatcaagga gtaggcgaat gagaaagttc atgccgatcg tcgtcgtggc
    12661 cgtagcggcc gcggcgtgct cgtcggtcaa ggaagtcgcg acccaggaca tgccgctgga
    12721 agtgcagcac cagattctcg acgagtacaa ggatcgctac gtgtggacgc gggtgcaggt
    12781 ccaggatttg ggcgaaggcg gtgttatccc gcgcgacgaa aaggtgaaaa tcgtcgacgt
    12841 cgggatgttc aaggccggtt cggtgacggt cgagacccta aagaagaaga accgtgtggt
    12901 ccagggcttg agcctcgaca agccgctcac caaggccaaa atcgactccg cgctgaccga
    12961 atacttctgg ttcgacgatc ccacgctgcg ccacgtcgcc ttcattcgga agttcggcaa
    13021 ggacacggcc aagctgatca tgaaccacga cctggtggcc ggcatgtcgc ccgaggcggc
    13081 actggcgtcg tggggcaaac ccgccaagac cgagcgctcc gagcgcaacg gtgtcgtcca
    13141 ggagcgctgg cgctacccga ccgcccaggt gggacgcacg aaggacattc tgttcgaaaa
    13201 tgggaaggtc aggagctggg accagtagaa ctccgctgac cgccggttac acgaaaggcg
    13261 cccgagcaat cgggcgcctt tttttctgtc gccgggcga
//
LOCUS       DASPGK010000282         8282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_308841, whole genome shotgun sequence.
ACCESSION   DASPGK010000282 DASPGK010000000
VERSION     DASPGK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308841"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VEC56_07805"
     CDS             complement(<1..298)
                     /locus_tag="VEC56_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14095.1"
                     /translation="MQSRAPHTARAGEFTATPPDGRILLVSCYELGQEPLGITVPAGV
                     LARAGHPARLLDLAVSSIDIDAIERADLVAVSTPMHTALRLGTQVAAIVRRVNPD"
     gene            387..2432
                     /locus_tag="VEC56_07810"
     CDS             387..2432
                     /locus_tag="VEC56_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXV14096.1"
                     /translation="MATPESHHLRSAKLKPGVSPDQLDRPREADGNADAQRVIRGNAL
                     IFWDPKRPGHKLDAIDTDQITPAKDCVSESLDALDKRWKEGAFRFLMPDFRARVHRGE
                     TFLVAGDRFAIGSSREMSPAGLRAIAEEAGLEMVVVCGHNVGDIFRRNALNLGLEVVQ
                     SADAVADARDGDRFAYVPATRRLTNETRGKTYEPVGLTPKEDEIRRSGGIFAMGRREF
                     RESVERAPSVEWPDEATARGLTTTEQIAWAHRVDKDARVEPGRTLRVFADLLPASDGT
                     APFAIHTFNQIARGAVFPRQAAIANDHFVFTGADADEKQTRIGRAFAEQNALEKPFYA
                     TPGDGIFHFYFPEQGLIYPGQFIPGADSHSRAYGAYGAVGIGVGSTTLGFGWATGYVY
                     FTPARARRVVFTGKLQPWVTGKDVVLKLLEGWGAKQAQGMSVELVDANLQLPIAYRNT
                     IANMMAEAEAQNGIFAPDDITDAWYASKGFADLPYPRVTPGANARYDIDETLDLSTVV
                     PMVAKPFSPGNAFPADVIARERIGFDKAFIGSCTNGSYDDLLQAALVVKRAREAGFTK
                     AEKPLIVFPGSGGVKRLIERPDARLGGESVADVLRAIGAEIRQSWCGPCFGQGPDALK
                     RGERAITSFNRNWQNRMGVGGEGYLASPSVVVASALAGYMAPPSELGIEWSAEAFAV"
     gene            complement(2429..3013)
                     /locus_tag="VEC56_07815"
     CDS             complement(2429..3013)
                     /locus_tag="VEC56_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012163654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HXV14097.1"
                     /translation="MTPSPRVVTLAASLREGSFNRRLVRIAADGLDAAGAVATRLDLR
                     DYPLPLYDADLEKWDGLPRNALALKEVFAAHDAILFALPEYNGSVTPLFKNTIDWASR
                     PTPGEKTGLAWARNKPCALVSASNGALGGLRGLYHARWVLQTCGLIVIPQQKALSRAA
                     TAFDANGDLVDTKERDAVYRVAAALVELTAKLVR"
     gene            3077..4045
                     /locus_tag="VEC56_07820"
     CDS             3077..4045
                     /locus_tag="VEC56_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV14098.1"
                     /translation="MEYRRLGRSGLKVSAISLGAWVTYGAQVGEDVAYACMTEAFEHG
                     VNFFDNAEAYASGKAEVVMGNVIRRAGWKRSDLVISTKLFWGGKGPNDKGLSRKRVVE
                     GTERSLSRLQLDYVDLLFCHRPDPETPIAETVHAMNHVIAQGKALYWGTSEWSADEIA
                     EAAAIAARANLLGPATEQPQYHMFHRDRVEKEYARLYDDIGLGTTIWSPLASGLLTGK
                     YDQGPVKGTRAELPGMEWLREELVGESARPRIEKVKKLKAVADQLGCSRAQLAIAWCL
                     KNPRVSSAITGATKREQVTENLGALAVVERLTPDTMKDIDRILSGR"
     gene            4388..6880
                     /locus_tag="VEC56_07825"
     CDS             4388..6880
                     /locus_tag="VEC56_07825"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXV14099.1"
                     /translation="MNQPSSRSRPDSIAVSAPDPLADAYRRWGYLMADLDPMGRIAPY
                     EHSDIAEARTTADAREVDAWRSLYSGKIGFEFMHMVEKERVDWMRDAIEAGPPAPDAN
                     WILERLMETELFEKFLHTKYVGSKRFSVEGVAALIPLLDSILDASGGLGTEYLYIAMS
                     HRGRLNVMKSIVNLPIEDIFAGIEDVDPRSVLGSGDVKYHIGATGRYRTHSGRDIEVH
                     LSTNPSHLEAVDAVIMGRVRARQERWGATGIERILPVTLHGDAAFAGQGIASETLNFA
                     DIPHYEVGGTIHVIVNNLIGFTAEPVALHSTRFSSEAAKRVSMPVIHVNAEAPGAVWW
                     AGKLAAAYRRRFHSDVLVDVIGFRRYGHSEIEDPTITQPALYDKIAKHPLAWEVYAKE
                     LGLDVATIETMKTRATAGLREGQEKGRQYERRPVFWKLPEFWGRYAGGPYDPGYEVDT
                     AIPIERVREIGARITSVPDGFTPHPKIAKLLQQRVEMAQGTRKADFGMAEALAFGSLV
                     TSGIRIRLTGQDSRRGTFNHRHAALIDVRTSEAYMPLAHIADGQAEFVAVDSPLSEAA
                     AMGFEYGYSREFPDALVLWEAQFGDFANGAQIIIDQFVSAGEDKWSLLSGLVLLLPHG
                     HEGQGPEHSSARIERFLQLAAHDNMQICQPSTAAQYFHLLRRQALRAWRKPLVVFTPK
                     SMLRADAACSHVDELANGAFRSVLGDTEAKNVRAVLVCSGKIAHELRAERAAQGAMDR
                     AIVTIEQFYPFPEAAVAEALRAFPETAKIVWVQEEPANMGALAYVRPRLKSAVGGRHV
                     FSIKRYESASPATGSAKAHALEQKALIKLAFA"
     gene            7025..>8282
                     /locus_tag="VEC56_07830"
     CDS             7025..>8282
                     /locus_tag="VEC56_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXV14100.1"
                     /translation="MRLKPLNKIRESFSAKVTLLVAASVMATSFVVGLATTHSTGNFL
                     TEQLKEQFPSTLTTTTTRLRLWHDRQLTELSRLAEANALRDNVDRYAKADNEAAAAAA
                     REELSHYLRLVRRNYPGYEELLVLDSSGRTVASAGGTAANASESAEEAWLELGEAGFE
                     PGASRPIASERGVHQWVFVTTREGSVARLKQTWLAARVDLTQCGQILAEVKLGPGGDL
                     FLLDAQGRFLTQPRKATIPKVGMEAMKVPTRQSGPIIVERRDTYDNRSAFHSKVHLDE
                     PGWWLVYEEDYTAAMSPVIEAQRRIWVAVLAVGAIAILAALRIAQSFLKPIHELKLGA
                     KRINEGLVGVKIPKRFDDEVGMMIDTFNEMASRITLWQAELQYKNKMLNTQNDRLQEM
                     NERLEKLSVTDGLTGLFNHRHFWNLMN"
BASE COUNT         1463 a         2707 c         2833 g         1279 t
ORIGIN      
        1 cgtcggggtt gacgcggcgc acgatcgccg cgacctgcgt gcccaggcgc agcgccgtgt
       61 gcatgggcgt cgagacggcc accaagtccg cgcgctcgat cgcgtcgatg tcgatggagg
      121 agacggccag gtccagcagg cgtgccgggt gccccgcgcg cgcgaggacg ccggccggga
      181 ccgtgattcc gagcggctcc tgcccgagct cgtagcacga caccaggaga atgcgaccgt
      241 ccggcggggt tgcggtgaat tcaccggcgc gggcggtgtg tggggcacgc gactgcatgt
      301 cgcgagtata aggaggtggc gcgcacgcgc gcaacgtcgc cgcgccgccc cacccgggaa
      361 tcgtgtatac tgcgccggag gtacccatgg ccacaccgga gtcgcaccac ctgcggtcgg
      421 cgaagctcaa acctggagtc tcgccggacc agctcgaccg gccgcgcgag gcggacggca
      481 acgccgatgc gcagcgcgtc atccgcggta acgcgctgat cttctgggac cccaagcggc
      541 ccggccacaa gctcgacgcc atcgacaccg accagattac ccccgccaag gattgcgtct
      601 ccgagagcct cgacgccctc gacaagcgtt ggaaggaggg cgcgtttcgc ttcctgatgc
      661 ccgatttccg cgcgcgcgtg caccgcggcg agaccttcct ggtggccggc gaccgcttcg
      721 cgatcgggtc gtcgcgcgag atgagcccgg ccggattgcg cgccatcgcc gaggaagccg
      781 ggctcgagat ggtggtcgtg tgcggccaca acgtcggaga catcttccgg agaaacgcgc
      841 tcaacctcgg gctggaagtg gtgcagagcg ccgacgcggt ggccgacgcg cgcgacggcg
      901 accggttcgc gtacgtcccg gcaacgcgac ggctgaccaa cgagacgcgc ggcaagacgt
      961 acgagccggt cgggctgacg cccaaggagg acgagattcg ccgctcgggc ggtatcttcg
     1021 ccatgggccg gcgcgagttt cgcgaatccg tggagcgcgc gccgtcggtg gaatggcccg
     1081 acgaagcgac cgcgcgggga ctgaccacca ccgagcagat cgcgtgggcg caccgcgtcg
     1141 acaaggacgc gcgcgtcgag ccggggcgca cgctgcgcgt gttcgcggac ctcctgcccg
     1201 catcggacgg caccgccccc ttcgcgatcc acaccttcaa ccagatcgcg cgcggcgcgg
     1261 tgttcccgcg ccaggccgcg atcgcgaacg accacttcgt gttcacgggc gccgacgccg
     1321 acgagaagca gacgcgcatc gggcgcgcgt tcgcggagca gaacgcgctc gagaagccct
     1381 tctacgccac gcccggcgac ggcatcttcc acttctactt tcccgagcaa ggcctgatct
     1441 atccggggca gttcattccc ggggcggact cgcattcgcg cgcgtacggg gcctacggtg
     1501 cggtcgggat cggcgtgggg tcgacgacac tcggcttcgg gtgggcgacg gggtacgtgt
     1561 atttcacgcc cgcgcgcgcg cgccgcgtcg tgttcaccgg caagctgcag ccgtgggtga
     1621 cgggcaagga cgtcgtattg aaacttctcg agggatgggg agccaagcag gcgcagggca
     1681 tgtcggtcga gctggtcgac gccaacctcc agctgcccat cgcgtaccgc aacaccatcg
     1741 ccaacatgat ggccgaagcg gaagcgcaaa acggcatatt tgcgcccgac gacatcaccg
     1801 acgcgtggta cgcgtccaag ggcttcgccg atctgccgta cccgcgtgtg acacccggcg
     1861 cgaacgcgcg ctacgatatc gacgaaacac tcgacctctc gacggtggtg ccgatggtgg
     1921 ccaagccgtt cagccccggc aacgccttcc cggccgacgt catcgcgcgc gagcgcatcg
     1981 gcttcgacaa ggcgttcatc gggtcgtgca ccaacggcag ctacgacgac ctcctgcagg
     2041 cggcgctggt cgtcaagcgc gcgcgcgaag ctggattcac caaggcggag aagccgttga
     2101 tcgtgttccc ggggtcgggc ggggtcaagc gcttgatcga gcgtcccgat gcgcgcctgg
     2161 ggggcgagtc ggtggcagat gtcctgcgcg ccatcggcgc ggagattcgc cagtcgtggt
     2221 gcggtccctg cttcggtcag gggccggacg cgctgaagcg cggcgagcgc gccatcacgt
     2281 cgttcaatcg caactggcaa aaccggatgg gcgtgggtgg cgaagggtat ctcgcgagtc
     2341 cgtcggtggt cgtggcctcg gcgctggccg ggtacatggc gccccccagc gagctgggaa
     2401 tcgagtggag cgcggaggcg ttcgcggtct agcgaacgag cttggccgtc aactccacca
     2461 gcgcggcggc gacccggtag accgcgtcgc gctccttggt gtccaccaag tcgccgttcg
     2521 cgtcgaacgc ggtggcggcc ctcgagagcg ccttctgttg cggaatcacg atcaagccgc
     2581 aggtttgcag gacccagcgc gcgtgataga gaccgcgcag tccgccgagt gcgccgttgg
     2641 atgccgacac cagcgcgcac ggcttgttgc gcgcccacgc gagtcccgtt ttctccccgg
     2701 gggtgggccg cgacgcccaa tcgatcgtgt tcttgaacag cggtgtcaca ctgccgttgt
     2761 actcgggcag cgcgaacagg atcgcgtcgt gcgcggcgaa cacctccttg agcgcgaggg
     2821 cgttgcgcgg caagccgtcc cacttttcca ggtccgcgtc gtagagcggg agcgggtagt
     2881 cgcgaagatc caagcgcgtc gcgaccgcgc cggcggcgtc caacccgtcg gcggcgatac
     2941 gcacgagcct gcgattgaag gagccctcgc gcagactggc ggccagggtg acgacgcggg
     3001 gtgagggagt catcgcggta gtatcgcgcg cgacggcacg gtcgtcaatc gaccgaccac
     3061 ttcgtaagga gcaggcatgg agtaccggcg actgggacga tcgggtctca aggtgtccgc
     3121 gatttcgctc ggcgcgtggg tgacgtacgg cgcgcaagtg ggcgaagacg tcgcgtacgc
     3181 gtgcatgacc gaagcgttcg agcacggcgt caatttcttc gacaacgcgg aagcctacgc
     3241 gtcggggaaa gcggaagtgg tgatgggcaa cgtgatccgt cgcgcgggct ggaagcgctc
     3301 ggacctggtg atatcgacca aactgttctg gggcggcaag gggcccaacg acaaggggct
     3361 ctcgcgcaag cgcgtcgtcg agggcaccga gcgatcgctt tcgcgcttgc agctcgacta
     3421 tgtcgacctc ctgttctgcc accgccccga ccccgagact cccatcgcag agaccgtgca
     3481 cgccatgaac cacgtgatcg cccagggcaa ggcgctgtac tgggggacga gcgagtggag
     3541 cgccgacgag atcgcggaag cggccgcgat cgcagcacgc gcgaacctcc tggggcctgc
     3601 aaccgagcag ccgcagtacc acatgttcca ccgcgaccgc gtcgagaagg agtacgcgcg
     3661 cctctacgac gacatcgggc tgggcacgac gatctggagc ccgctggcga gcgggctctt
     3721 gaccggcaag tacgaccagg ggccggtgaa gggaacgcgc gcggagctcc cggggatgga
     3781 gtggctgcgc gaagagctgg tcggggaatc ggcgcgtccc cggatcgaaa aggtcaagaa
     3841 gctcaaagcg gtcgccgacc aactgggatg ttcgcgcgcg cagctcgcga tcgcgtggtg
     3901 cttgaagaat ccccgcgtga gctcggcgat caccggcgcg acgaagcgcg agcaggtgac
     3961 ggagaatctg ggcgcgctcg cggtcgtgga acggttgaca cccgatacca tgaaggacat
     4021 cgaccggatt ctgtcggggc ggtagggcaa aaaaagatcc cgggcgaaac ccgggatctt
     4081 ctttaagcgc tcaggaactg aacgccatag tgcagggagc gctacaatag cacgcggcct
     4141 ccgcgggatc aagcatcttt ttgtgttgtc cggttatccc attgtggtag aatgatttcg
     4201 gtgttcaggt agggtccggc cggggacaac cggccgggcc gaaaaagcgt tgtttgacac
     4261 gcgaaaaagg cccgtgttaa ttaactgcgg ctagtcgcgg caattaagca cgtgtcgcgg
     4321 ggccgtttcg gcgacggagt gccgtcgccg gctggtcaga actgacaaga attgcgagca
     4381 tgaccgaatg aaccaaccgt cttcccgttc ccgtcccgac tctatcgccg tttccgcacc
     4441 cgatcccctc gccgacgcgt accgccgatg gggctacctc atggccgatc tcgacccgat
     4501 gggtcgtatc gcgccctacg agcactccga catcgcggag gcgcgtacga ccgccgatgc
     4561 gcgcgaggtg gatgcgtggc gctcgctcta cagcggcaag atcggcttcg agttcatgca
     4621 catggtggag aaagagcgcg tcgactggat gcgggacgcc atcgaagcgg gccctccggc
     4681 gcccgacgcc aactggattc tcgagcggct gatggagacc gagctgttcg agaagttcct
     4741 gcataccaag tacgtcggta gcaagcgctt ttcggtcgaa ggcgtggcgg cgttgattcc
     4801 gctgctcgat tccatcctgg acgcctcggg cgggctgggc acggagtacc tctatatcgc
     4861 catgagccac cgcggccggt tgaacgtgat gaagagcatc gtcaacctgc ccatcgagga
     4921 cattttcgcc ggcatcgagg acgtcgaccc gcgcagcgtg ctgggaagcg gcgacgtcaa
     4981 gtatcacatc ggcgccaccg gccgctaccg gacccattcg ggccgcgaca tcgaggttca
     5041 tctctccacc aatcccagcc acttggaagc cgtcgacgcg gtcatcatgg ggcgcgtgcg
     5101 cgcgcgccag gaacgctggg gtgccaccgg catcgagcgc attctgccgg tgacgctaca
     5161 cggcgacgcc gccttcgccg gccagggcat cgcgtcggag actttgaact tcgccgacat
     5221 cccgcattac gaagtgggag ggacgatcca cgtcatcgtc aacaacctga tcggattcac
     5281 cgccgagccg gtggcactgc acagcacgcg cttctcgtcg gaagcggcca agcgggtgtc
     5341 gatgccggtc atccacgtca acgcggaagc cccgggtgcg gtgtggtggg cgggcaagct
     5401 cgccgccgcg taccgccgcc ggttccacag cgacgtcctg gtcgacgtga tcggcttccg
     5461 gcgttacggc cacagcgaga tcgaggaccc gaccatcacg cagcccgcgt tgtacgacaa
     5521 gatcgccaag cacccgctcg cgtgggaagt ctatgcgaag gagctcgggc tggacgtggc
     5581 caccatcgag accatgaaga cgcgcgcgac cgcggggttg cgcgagggcc aggagaaggg
     5641 tcgccagtac gagcggcgcc ccgtgttctg gaagttgccg gaattctggg gtcgttatgc
     5701 cggcggcccc tacgacccgg gctacgaggt cgataccgcg atcccgatcg aaagggtgcg
     5761 cgagatcggt gcgcgcatca cgagcgtgcc cgacgggttc acgccccacc ccaagatcgc
     5821 gaagctcctc cagcagcgcg tcgagatggc gcagggcacg cgcaaggcgg acttcggaat
     5881 ggccgaggcg ctcgcgttcg gctcgctggt gacgtccggg attcgcatcc gcctgaccgg
     5941 acaggactcg cgacgcggca ccttcaatca ccgtcacgcg gccttgatcg acgtccgcac
     6001 gagcgaggcc tacatgccgc tcgcgcacat cgccgacggc caggccgaat tcgtcgccgt
     6061 ggacagcccg ctctcggagg ccgcggccat gggcttcgag tacgggtaca gccgcgagtt
     6121 cccggacgcg ctggtgctct gggaggcgca gttcggcgac ttcgccaacg gcgcgcaaat
     6181 catcatcgac cagttcgtga gcgcggggga agacaagtgg agcctgctct ccggcctggt
     6241 tctgctgttg ccgcacgggc acgagggcca gggccccgag cactcgagcg ctcgcatcga
     6301 gcgcttcttg cagctggccg cgcacgacaa catgcagatc tgccaacctt cgacggcggc
     6361 gcagtatttc catctcttgc gccggcaggc gctgcgggcg tggcgaaaac cgctcgtggt
     6421 cttcacgccc aagagcatgc tgcgcgccga cgccgcgtgc tcacacgtcg acgagctggc
     6481 aaacggcgca tttcggtccg tcctgggtga caccgaagcg aagaacgtgc gcgccgtgct
     6541 cgtctgctcc ggaaagatcg cgcacgagct ccgcgccgag cgcgcggcgc agggagccat
     6601 ggaccgcgcg atcgtcacca tcgagcagtt ttatccgttc ccggaggccg cggtggccga
     6661 agcgctgcgc gcgttccccg aaaccgcgaa gatcgtctgg gtgcaggagg agcccgccaa
     6721 catgggcgcg ctcgcttacg ttcgcccgcg cttgaagtcg gccgtgggcg ggcgccacgt
     6781 gttcagcatc aagcgctacg agagtgccag cccggcgacg ggttcggcca aggctcacgc
     6841 cctcgagcag aaggcgctga tcaagctcgc gttcgcgtag gggagcatcg ctccgagttg
     6901 ttcctcccgg cgacccgagt cgtaatccgg caaagttttc cctcccaggc ccgcgcgtgc
     6961 gcgggccttt gtgtactctt cttgctctcc caattgcgct tactcccgtt cgggagagct
     7021 cgctatgcgt ctcaaaccgc tcaacaagat tcgggaaagc ttctcggcca aggtgacgct
     7081 cctggtcgcg gccagcgtca tggccacgtc gttcgtggtc gggctggcca cgacccacag
     7141 cacgggaaac ttcctgaccg aacagctcaa ggagcagttc ccgtccacac tgacgacgac
     7201 gaccacgcgc ctgcgcttgt ggcacgatcg ccagctcacg gagctctccc gcttggcgga
     7261 agccaatgcg ttgcgcgaca acgtcgaccg ctacgccaag gccgacaacg aggccgcggc
     7321 ggccgcagcc cgcgaggagc tatcgcacta tttgcgcttg gtgaggcgga attaccccgg
     7381 ctacgaagag ctattggtgc tcgattcgtc gggccggacg gtggcgtcgg cagggggcac
     7441 cgcggcgaac gcgtccgagt cggccgaaga ggcgtggctc gagttgggcg aagccggctt
     7501 cgagccgggc gcctcgcgcc ccatcgccag cgagcgcggc gtgcaccagt gggtcttcgt
     7561 cacgacccgc gaaggcagcg tcgcgcgctt gaagcagacg tggctggccg cgcgcgtgga
     7621 cctgacacag tgcggtcaga ttctcgccga ggtcaagctc ggccccggcg gcgacctgtt
     7681 cctgctcgac gcccagggac gttttctcac ccagccgcgc aaggccacga taccgaaagt
     7741 cggcatggag gcgatgaagg tgcccacgcg tcaaagcggc cccatcatcg tcgagcggcg
     7801 cgacacctac gacaaccgca gtgccttcca cagcaaggtg cacctcgacg agcccggctg
     7861 gtggctcgtg tacgaagagg actacaccgc ggcgatgtcg cccgtcatcg aagcgcagcg
     7921 ccgcatttgg gtggccgtgc tggcggtggg tgcgatcgcg atcctggccg cgctgcgcat
     7981 cgcccagtcg ttcctgaagc cgatccacga gctcaagctg ggcgcgaaac gcatcaacga
     8041 aggcctggtg ggcgtcaaga tccccaagcg cttcgacgac gaagtcggca tgatgatcga
     8101 caccttcaac gagatggcgt cgcgcatcac gctgtggcag gccgagctcc agtacaagaa
     8161 caagatgctc aacacgcaga acgaccggct gcaagagatg aacgagcgcc tggagaagct
     8221 atcggtgacg gacggattga cggggctctt caaccaccgc cacttctgga acctgatgaa
     8281 ca
//
LOCUS       DASPGK010000283         4241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_541841, whole genome shotgun sequence.
ACCESSION   DASPGK010000283 DASPGK010000000
VERSION     DASPGK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541841"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1324
                     /gene="tyrA"
                     /locus_tag="VEC56_07835"
     CDS             170..1324
                     /gene="tyrA"
                     /locus_tag="VEC56_07835"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF008400.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HXV14101.1"
                     /translation="MTPLQHPAGDDARSLPVLRALIDAVDHEVLQLLSRRNGLVAEIA
                     AYKRAHGVAIRDHRREREIIADRRERSTPLGLQPDVIEGMYRLLLWASRDRQASLRAQ
                     VPLDIETKTVAVIGGRGAMGACMARLFADLGHAVMIADLDTELTPEVAAREADVVVIS
                     VPIDATVDVIRRIGPLVRADALFMDVTSIKTAPMQAMLASTRASVVGTHPLFGPSVHS
                     LQGQRVALTTGRGDEWTRWLTQMLEARGLTVVETTPEAHDRAMAVVQVLTHFSTEVAG
                     ATMAKLGVPLEATLPFTSPIYLMELLMTARHFAQSPDLYASIQMSNPETARVTEAFAR
                     TAEEMRANVAARDHDAFARVFDHVHDYFGEFTERALEQSSFLIDRLVERA"
     gene            1328..1951
                     /gene="trmB"
                     /locus_tag="VEC56_07840"
     CDS             1328..1951
                     /gene="trmB"
                     /locus_tag="VEC56_07840"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXV14102.1"
                     /translation="MGKTFTARRVHIEQRRIDRTYAGTVAELFPTHQPLEIEIGSGKG
                     RFLLHRAAAHRERNFFGIDYRWRFLKEGVLRAEQRGLSNLIFLKTEAEEIIPHLVPPS
                     SVEVFHIYFPDPWHKRKHHKRRLLTPEFFKRLHASLVPGGLLELATDNFDYMIAFKAA
                     LVEAGDTLWAGIRESRNERILDPGVQTHFEAKYARDGRDLYYIELRK"
     gene            2026..2736
                     /locus_tag="VEC56_07845"
     CDS             2026..2736
                     /locus_tag="VEC56_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14103.1"
                     /translation="MKKRIHLDIMRQPDDVSCGATCLHAIYRYHGDKIDLADVVRDVE
                     QLEEGGTLGVFLGCHALKRGYRVSLYSYNLQVFDPTWRTLGRGELIDRLQRQTAVKHQ
                     RKLKIASRAYIEFMEMGGEIRFAELSPKLMRRYLNGSIPMLAGLSSTYLYQSAREYGP
                     RGEYDDVRGVPGGHFVVLCGYDSSNRDVLVADPYQPNPAKERYYEVGVNRLICSVLLG
                     VLTYDANLLVVSPARNRS"
     gene            2740..4203
                     /locus_tag="VEC56_07850"
     CDS             2740..4203
                     /locus_tag="VEC56_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HXV14104.1"
                     /translation="MSRLFVVNNPKNWPLHIPGVEVVSARAYLTETAYSDIENAKVFN
                     LCRSYRYMSSGYYVSLLAEARGHRAIPSILTLQDMRTEAIFRIRSEDLDDLIQRSLAH
                     LKAERYQLNIYFGRTTVKRYERLAQQLFSFFEAPLLRAHFHYEKKKWELDQVYPIAVN
                     DIPDWQRPFVVNAADEYFVKLERRPRRKKSFRYDLAILYNPDETDPPSDPRALRKFCK
                     AAEAIGMDPEYIVRDDYRRVAEFDALFIRETTAVNHHTYRFARRAAAEGLAVIDDPES
                     IIRCTNKVYLAEMFDRYRLPTPKTMIVHRDNASQIADTLNLPCILKQPDSSFSQGVVK
                     LNTLMELREEVERRLESSDLLIAQEFMPTDFDWRVGVLEGKALYVCRYFMARRHWQIL
                     KRTKRGYLTSGKVETLAVARAPREVIRLATIAATAVGDGFYGVDLKQRGSDFYLIEVN
                     DNPSIEAGYEDKVIGDKLYARIMKSLLQRVERIKGQS"
BASE COUNT          769 a         1440 c         1293 g          739 t
ORIGIN      
        1 tggagttcag acgtgtgctc ttccgatctg gggcggatta cctaccgggg cggatccagt
       61 gtaaacccgt gggcgatcgc tggcaatccc ttcgtggcgc gaatcgctgt gctagaatcg
      121 acctgtccaa gccggaacac aacgcccgtt ccaaccgagt gagactacca tgactccatt
      181 gcagcatccc gcgggcgacg acgcccgttc gctccccgtc ctccgcgcct tgatcgacgc
      241 ggtggaccac gaagtcctac agttgctctc gcgccgaaac ggcctcgtgg ccgagatcgc
      301 ggcctacaag cgcgcgcacg gcgtcgcgat ccgcgaccac cggcgcgagc gcgagatcat
      361 cgccgaccgc cgcgagcgct cgacgccgct gggcttgcag ccagacgtga tcgaagggat
      421 gtatcggttg ctcctgtggg cgagccggga tcgccaggcg tccttgcgcg cgcaggtgcc
      481 gctcgacatc gagaccaaga ccgtcgccgt gatcggcggg cgcggtgcga tgggcgcgtg
      541 catggcgcgc ctcttcgccg acttgggtca cgccgtcatg atcgcggacc tcgacaccga
      601 gttgacgccc gaagtcgccg cgcgcgaggc ggacgtggtc gtgatcagcg tccccatcga
      661 cgccaccgtc gatgtaatcc gccgcatcgg ccctttggtt cgggccgatg cgctcttcat
      721 ggacgtcaca tcgataaaaa ccgctccgat gcaagcgatg ctcgcctcga cgcgcgcgtc
      781 ggtggtgggc acgcatcccc tcttcggccc gtccgtgcat tcgctccagg gccagcgcgt
      841 cgcgctcacc accggccggg gcgacgagtg gacgcgatgg ctcacccaga tgctcgaagc
      901 gcgcgggctc acggtggtag aaacgactcc cgaggcccac gaccgcgcga tggcggtggt
      961 gcaggtgctt acccacttct ccaccgaggt cgcgggtgcg accatggcca agctgggcgt
     1021 cccgctggag gccacgctcc cgttcacgtc gccgatctat ctcatggagc tcttgatgac
     1081 cgcgcgtcac ttcgcgcagt cgccggacct gtacgcctcg atccagatgt cgaacccgga
     1141 gacggcgcgc gtgacggagg ccttcgctcg cacggccgag gagatgcgcg ccaacgtggc
     1201 tgcgcgcgac cacgacgcct tcgcgcgcgt gttcgaccac gtccacgatt acttcggcga
     1261 attcaccgag cgcgcgctcg agcagtcgtc gttcttgatc gatcgcttgg tggagcgggc
     1321 ctagcgtatg ggcaagacct tcacggcccg gcgcgtccac atcgagcagc gccgcatcga
     1381 ccgcacgtac gcgggcacgg tggcggaact gttccccacc caccagccgc tcgagatcga
     1441 gatcgggtcc ggcaaggggc gctttctcct gcatcgcgcc gcggcgcatc gcgagcgcaa
     1501 cttcttcggc atcgactatc ggtggcgctt cttgaaggag ggcgtgctgc gcgccgagca
     1561 gcgcggtctc tccaacctga tcttcttgaa gaccgaagcc gaggagatca tcccgcacct
     1621 cgtgccgcca tccagtgtag aggtcttcca catctacttc cccgacccct ggcacaagcg
     1681 gaagcaccac aagcgacgct tgctgacacc ggagttcttc aagcggctcc acgcctcgct
     1741 cgtccccggc ggcttgctcg agctcgcgac ggacaacttc gactacatga tcgcgttcaa
     1801 ggcggcgctg gtcgaagcgg gagacacgct gtgggccggc atacgcgaga gccgcaacga
     1861 gcgtatcctc gaccccggcg tgcagacgca cttcgaggcc aagtacgcgc gagacggacg
     1921 cgatctctat tatatagagc tgcggaaata gccgcgccgt tggcgtcgcg cctccatcat
     1981 atcgagcttt gcaagtagtc gaggcgcggg ctagaatctc cgccaatgaa aaagcgcatc
     2041 catctcgaca tcatgcgcca gcccgacgac gtgagctgcg gtgccacctg tctgcacgcg
     2101 atctatcgct accacggcga caagatcgac cttgccgacg tcgtgcgcga cgtcgagcag
     2161 ctcgaagagg gcgggacgct gggcgtcttc ttgggctgcc acgccttgaa gcgcggctac
     2221 cgcgtgagcc tgtactccta caacctccag gtcttcgacc ccacctggcg aaccctcggg
     2281 cgcggcgagt tgatcgaccg cctccagcgg caaaccgcgg tcaagcacca gcgcaaactc
     2341 aagatcgcct cgcgcgcata catcgaattc atggagatgg gcggcgagat tcgtttcgcg
     2401 gagctctcgc ccaagctcat gcgtcgctat ctcaacggat cgattccgat gctcgcgggc
     2461 ctgagctcca cgtacctcta tcagagcgcg cgcgagtacg ggccgcgcgg cgagtacgac
     2521 gacgtgcgcg gggttccggg cggacacttc gtcgtgctgt gcggctacga ctcgtcgaac
     2581 cgcgacgtcc tggtggccga tccctaccag cccaatcccg ccaaggagcg ttactacgaa
     2641 gtcggcgtca accgtcttat ttgttccgtt ctcttgggcg tgttgactta cgacgccaat
     2701 ttgctggtcg tttcgcccgc gcgcaaccgg agctagacca tgtcgcggct cttcgtcgtc
     2761 aacaatccca agaactggcc gctccacatt cccggcgtcg aagtggtgtc ggcgcgcgcg
     2821 tacctgaccg aaaccgcgta cagcgacatc gagaacgcga aggtgttcaa cctgtgccgg
     2881 tcgtatcgct acatgagctc gggctactac gtctcgctgc tggccgaagc gcgcggccat
     2941 cgcgccatcc ccagcattct gaccctccag gacatgcgca ccgaggcgat tttccgcatt
     3001 cgctccgagg acttggacga cttgatccag cgctcgctcg cgcatttgaa agccgagcgc
     3061 taccagctca atatctactt cggcaggacc acggtgaagc gctacgagcg cctggcgcag
     3121 cagctctttt ccttcttcga agcgccgctg ttgcgcgcgc acttccacta cgaaaagaag
     3181 aagtgggagt tggaccaggt ctatccgatc gcggtcaatg atattccgga ctggcagcgg
     3241 cccttcgtcg tcaacgcggc cgacgaatat ttcgtcaagc tcgagcgccg gccgcgccgc
     3301 aagaagagct tccgctacga cctcgcgatc ctctacaacc ccgacgagac cgacccgccg
     3361 tccgacccgc gcgcgctgcg caagttctgc aaggcggccg aggcgatcgg gatggacccc
     3421 gagtacatcg tgcgcgacga ctaccgccgc gtagccgagt tcgacgcgct ttttattaga
     3481 gagacgaccg cggtcaacca ccatacctat cgctttgcgc ggcgcgccgc cgcggaaggc
     3541 ctggccgtca tcgacgaccc cgagtccatc atccgctgca ccaacaaggt ctatctggcc
     3601 gagatgttcg accgctaccg gctccccacg cccaagacga tgattgttca ccgcgacaac
     3661 gcgagccaga tcgcggacac gctgaacctg ccgtgcatct tgaaacaacc cgacagctcg
     3721 ttctcgcaag gggtggtaaa gctcaacacc ctcatggagc tgcgcgagga agtcgagcgg
     3781 cgcctcgaaa gctccgactt gctcattgct caggagttca tgcccaccga cttcgactgg
     3841 cgcgtgggcg tactggaagg caaggcgctc tacgtgtgcc gctacttcat ggcgcgacgc
     3901 cattggcaga tcctgaagcg caccaagcgc ggctacttga cgagcggcaa agtcgagacg
     3961 ctggccgtcg cgcgcgcgcc ccgcgaagtc atccgcctcg ccaccatcgc ggccaccgcc
     4021 gtcggcgacg ggttctacgg cgtggatttg aagcagcgcg gatcggactt ctacctgatc
     4081 gaagtcaacg acaacccgag catcgaggcg ggctacgagg acaaggtcat cggcgacaag
     4141 ctctacgcgc gcatcatgaa gtcgctcctg cagcgcgtcg agcgcatcaa gggtcagtca
     4201 tgacgctcgg gttgttcgaa ggcgtcgggc tcgaactgga g
//
LOCUS       DASPGK010000284         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1009218, whole genome shotgun sequence.
ACCESSION   DASPGK010000284 DASPGK010000000
VERSION     DASPGK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009218"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VEC56_07855"
     CDS             <1..1007
                     /locus_tag="VEC56_07855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXV14105.1"
                     /translation="HPDVIPHLGGIALLLGVLAGIALAAGANQNVIMRALPATLLVVL
                     LGIIDDTRNLAARRKLVVQVVAAALLVSVGFRLFTGIDAIDSLAIVAGAVSLAFIVGM
                     LSSVNLVDGHDGLAAGVAFIAAIAFAVIGVMDGAEGVVAGSLAIAGACLGFLLFNFPP
                     ARIYMGDTGAMLLGWTLAQIACLITMREPTVNSFAAVMLVLGVPILDTLLAVARRIVL
                     RAPLFAADHLHVHHVLRHSGLSPRKTLLVLYGLQAVLAGLGIAAASGSAVPVVVGLAV
                     VTVAFVVFLRAMVDGRAAESIAAETVPQPVARAAASASISFQSNFSTNIAERRTSVGR
                     "
     gene            1141..2064
                     /gene="hemC"
                     /locus_tag="VEC56_07860"
     CDS             1141..2064
                     /gene="hemC"
                     /locus_tag="VEC56_07860"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXV14106.1"
                     /translation="MTTKTTCRIVSDVDLLSRVQLEEVVRMLEGYGSKHAIDFVTPEE
                     PESLKLLRAAPGSPERMAVLMSVLLDRECDVLVLDASALPTKMPAGLTIGALTRRITP
                     YDALISNDDCILDELRENATLVTSSMRREAQLRYYRPDLKIARMQGSIDAVLQKVKSA
                     KVDCAVVAAADVERLSKQEFVAELLTNSVCVPAAGQGALAVLIRSSEDQFKDTVQSIN
                     DPATHGMLRAEWSFLEHLEVDPAAPVAVLASIEGKALELEGMVAFPDGSEKIQCMVKG
                     TIGNEEDLGRTLAVELLESGGREVIEEFRLT"
     gene            2081..>2633
                     /locus_tag="VEC56_07865"
     CDS             2081..>2633
                     /locus_tag="VEC56_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXV14107.1"
                     /translation="MIDRRLIREDPERVRNGLARRRAAVDVEQLLSIDREHLDNLQLS
                     EKLKHERNVASEEIARKKQKGEDITPLHAAMKETSSKIKALEARNKEIEEQLDSLLLT
                     VPNLPHASVPDGADAASNQVVRTVGAAKKPFDDVLPHWEIGEKLGILDLEAGRIVSGR
                     GFIVFRGDGALLCRALIQLMLDMH"
BASE COUNT          407 a          919 c          852 g          455 t
ORIGIN      
        1 ttcacccgga cgtcatcccg cacctgggcg ggatcgcgct cttgctcggc gtcttggccg
       61 gcatcgcgct cgcggccggg gccaatcaga acgtgatcat gcgggcgttg cctgccacct
      121 tgctcgtcgt gctcttgggt atcatcgacg acacgcgcaa cctcgcggcg cggcgcaagc
      181 tggtggtgca agtcgtcgcg gccgccctat tggtctcggt cggcttccgt ctcttcaccg
      241 gcatcgatgc catcgattcg ctcgcgatcg tcgcgggcgc ggtgtcgctc gcgttcatcg
      301 tcggcatgtt gagctcggtg aacttggtcg acggccacga cggactggcc gcgggggtgg
      361 cgttcatcgc cgcgatcgcc ttcgccgtca tcggcgtcat ggacggcgcc gaaggcgtgg
      421 tggccggctc gctcgcgatc gcgggcgcgt gcctcgggtt cctcttgttc aacttcccgc
      481 ccgcgcgcat ctacatgggc gacacgggcg cgatgttgct cgggtggacg ctggcccaga
      541 tcgcgtgcct cattaccatg cgcgagccga cggtgaactc gttcgccgcg gtgatgctgg
      601 tgctgggcgt tcccattctg gacaccctgc tcgcggtggc gcgccgcatc gtgctgcgcg
      661 cgccgctctt cgcggccgat catttgcacg tgcaccacgt gctgcgccac tccggtttgt
      721 ccccgcgcaa gaccctgctg gtgctgtacg gcctgcaggc ggtcctggcc ggcctgggca
      781 tcgcggcggc ctcgggcagc gcggtgccgg tcgtggtcgg actcgccgtc gtgacggtcg
      841 ccttcgtcgt gttcctgcgc gcgatggtcg acggccgcgc cgcggaatcg atcgccgccg
      901 aaacggtgcc gcagccggtc gcacgcgccg ccgcatcggc gtcgatctcg ttccagtcga
      961 acttctcgac caacatcgcc gagcgccgca cctccgtcgg tcgatagtct ctcgtcgggc
     1021 agcaccgccg cgcgaacaca cgcccgccct ccgttgaccc gcccctagcg ctgtgctatc
     1081 gtttcgccca cttgccggcg ggcgcgcagt gcaccctctc ccacgacgga tggaacccgt
     1141 ttgacgacga aaacgacgtg tcgcatcgtg agtgacgtcg atctcctctc gcgcgtccag
     1201 ctcgaggaag tcgtgcggat gctcgagggg tacgggtcga agcacgcgat cgacttcgtc
     1261 acccccgaag agcccgagtc gctcaagctc ctgcgcgcgg cacccggctc gcccgagcgc
     1321 atggcggtcc tgatgagcgt gctgctcgac cgcgagtgcg acgtgctggt gctggatgcc
     1381 tccgcgctgc cgaccaagat gccggccggg ctcaccatcg gggcgctcac gcgccgcatc
     1441 accccctacg acgcgctcat ctccaacgac gattgcatcc tcgacgagct gcgcgagaac
     1501 gccaccctgg tcacgagttc gatgcgccgg gaggcccagc tgcgctacta ccgccccgac
     1561 ttgaagattg cgcgcatgca gggcagcatc gacgccgtgc tgcaaaaggt gaaaagcgcc
     1621 aaggtcgact gcgcggtggt ggccgccgcc gacgtcgagc gcttgagcaa gcaggagttc
     1681 gtggccgaac tgctgaccaa ctcggtgtgc gtgccggccg cggggcaggg agcgctggcc
     1741 gtgctcattc gctcgtccga agaccagttc aaggacaccg tgcagtcgat caacgacccc
     1801 gcgacccacg gcatgctgcg cgccgagtgg tcgtttttgg agcacctgga agtcgacccc
     1861 gccgcgccgg tggcggtgct ggccagcatc gagggcaagg ccctcgagct ggagggcatg
     1921 gtcgcgttcc ccgacgggag cgagaagatc cagtgcatgg tcaaggggac catcggcaac
     1981 gaagaagacc tcggtcgcac gctcgccgtg gagctcttgg agtccggtgg acgcgaagtg
     2041 atcgaagagt tccgccttac ctaaccacgc gaagctctct gtgatagacc gccgtctcat
     2101 tcgcgaggac cccgagcgcg ttcgcaacgg gcttgcgcgc cgccgcgccg cggtggatgt
     2161 cgaacaacta ctctccatcg accgcgaaca cctcgacaac ctccagctct ccgaaaagct
     2221 caagcacgag cgcaacgtcg cgtccgagga gatcgcccgc aagaagcaga agggcgaaga
     2281 catcacccct ttgcacgccg ccatgaagga gacgtcctcg aagatcaagg cgctcgaggc
     2341 gcgcaacaag gagatcgaag aacagctcga ttcgcttctc ctcacggttc ccaatctccc
     2401 gcacgcctcg gtgcccgacg gcgccgatgc cgcatccaac caagtcgtgc gcaccgtcgg
     2461 tgccgccaag aagcccttcg acgacgtgct cccgcactgg gagatcggcg agaagctggg
     2521 catcctggac ctcgaagccg gccgcatcgt ctccgggcgc ggcttcatcg tgtttcgcgg
     2581 cgacggcgcg cttctgtgcc gcgcgctcat tcaactcatg ctcgatatgc acg
//
LOCUS       DASPGK010000285         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_776511, whole genome shotgun sequence.
ACCESSION   DASPGK010000285 DASPGK010000000
VERSION     DASPGK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776511"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1459)
                     /locus_tag="VEC56_07870"
     CDS             complement(56..1459)
                     /locus_tag="VEC56_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXV14108.1"
                     /translation="MSLVGVSTRMPTIAVVGAGFSGVATAANLARLPWPRGVRIVLLE
                     RGPRFARGVAYSTDHPLHLLNVPAGRMGALADDEGHFLRWLNRTEPTIKGDTFVPRRR
                     YGEYLEWLLDDATARAASNVEIVRRAGDVVDVVVSDARATVKLRDGESIDADRVVLAL
                     GNLPPRDPFASVLESDARYARDPWAPGALASLPRTRNPVLLVGSGLTMVDAVLALRDA
                     GHDSGFIAVSRHGMLPHSHRDWPAKPPVVEPPRALEDWDGSAGSIVRMVRVSSRDARR
                     SGLDWRDVINGLRPITPQLWHRMPPRERARFVRHVRPFWETHRHRMSTRVAEEINGLI
                     ARGELAIVAGRVAEASTGAQSITVTVRLRGSAATARYETGAVVNCTGPDGDFARLREP
                     LVAALRERGVLVPDALGLGLVADANGALIDAQGRASSVLFTLGTPRRADAWESTAVPE
                     LRAQAERLSHRLRESLP"
     gene            complement(1499..>2047)
                     /locus_tag="VEC56_07875"
     CDS             complement(1499..>2047)
                     /locus_tag="VEC56_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14109.1"
                     /translation="EVDVLLKAMYSDAGDTGAIKRCEVAGKGRDGSVKLVFGQFPAEV
                     ATFAEVGGGYDVFVSKNTLKRGYIHPEQQVDPRMLVHLRVDDETYVKAVFDLLKPGGF
                     ALIYNLSPAPAKEGEPYKPWADGRSPFTRELYERVGFAVVAFDLDDSAAAREMGKALG
                     WAEGMDLEKDLFGTYTLLRKPQ"
BASE COUNT          358 a          724 c          666 g          299 t
ORIGIN      
        1 actacgagaa ctacctggcc gacctacgca agaaattcca cgtcgagatt cgcagctagg
       61 ggagcgactc gcgaagccgg tgcgataacc gctcggcctg cgcgcgcagc tccggcaccg
      121 ccgtcgattc ccacgcgtcc gcgcgacgcg gagtccccag cgtgaacaac accgacgacg
      181 cgcgtccctg cgcgtcaatg agcgccccgt tcgcatcggc gacgagacct agcccgagtg
      241 catccggcac caatactcca cgctcgcgca gcgccgccac cagcggctcg cgcaggcgcg
      301 cgaagtcgcc gtcgggtccg gtgcaattga cgaccgcccc ggtttcgtag cgcgccgtcg
      361 cagcagagcc tcgcagacgt acagtcacgg tgatcgactg cgcgcccgtg ctagcctccg
      421 ccacccgccc cgccacgatc gcaagctcgc cgcgcgcgat cagcccgttg atctcctcgg
      481 cgacgcgcgt cgacatgcgg tggcgatgcg tctcccaaaa cggccgcacg tggcgaacga
      541 agcgcgcgcg ctcgcgcggc ggcatgcggt gccacaactg cggggtgatg ggacgcagac
      601 cattgatcac gtcgcgccag tccaggcccg aacgacgcgc gtctcgagac gagacccgaa
      661 ccattcgcac gatcgatccg gccgaaccgt cccagtcttc cagcgcacgc ggcggctcga
      721 ccacgggcgg cttggcgggc cagtcgcggt gcgagtgcgg gagcatgccg tgtcgcgaca
      781 ccgcgatgaa gccggagtcg tggcccgcgt cgcgaagcgc cagcaccgcg tcgaccatgg
      841 tgagcccgga gcccaccaac agaacggggt tcctcgttcg ggggagactc gcgagcgcgc
      901 ccggcgccca cggatcgcgc gcgtagcgcg cgtcgctctc tagaacagag gcgaaggggt
      961 cgcgcggcgg gagattcccg agtgccagca ccacgcgatc cgcgtcgatc gattcgccgt
     1021 cgcggagctt taccgtcgcc cgcgcgtcgg agacaacgac gtccacgacg tcgcccgcgc
     1081 ggcgcacgat ctccacgttg gaagccgcgc gcgcggtggc gtcgtcgagg agccactcga
     1141 gatactcgcc gtaacgccgg cgggggacga aggtgtcgcc cttgatcgtc ggctcggtgc
     1201 ggttgagcca gcgcaagaag tggccctcgt cgtcggcgag agcacccatg cgaccggccg
     1261 gtacgttgag aagatggagg gggtggtcgg tcgagtaggc gaccccgcga gcgaagcggg
     1321 gtccgcgttc caagagaacg atacggaccc cgcgcggcca aggcagcctt gccaggttgg
     1381 cagcggtagc aaccccgctg aacccggccc cgacgacggc aatggtgggc attcgcgtag
     1441 acactccgac taaactcata ggagagtcta atcgcactcc tattggagtg tcaagtgcct
     1501 attgcggctt ccggagcagg gtgtacgtcc cgaacaggtc cttttcgagg tccattccct
     1561 cggcccaacc cagggcctta cccatctccc gggccgccgc cgagtcgtcc aagtcgaagg
     1621 ccaccactgc aaagcccacc cgctcataga gttcgcgcgt gaagggcgag cgcccgtcgg
     1681 cccagggttt gtagggctcg ccttccttgg ccggtgccgg cgagaggttg tagatcaagg
     1741 cgaagccgcc cggcttcaac aggtcgaaga ccgccttcac gtaggtctcg tcgtcgaccc
     1801 gcaggtggac cagcatgcgc gggtcgacct gctgctcggg gtgaatgtag ccccgcttga
     1861 gcgtgttctt cgaaacgaag acatcatagc cgccgcccac ttccgcaaac gtcgcgacct
     1921 cggcggggaa ctggccgaac accagcttca cactgccgtc gcggcccttg cccgccacct
     1981 cgcagcgctt gatcgcgccc gtgtcgcccg cgtccgagta catcgctttc aagagcacgt
     2041 ccacttc
//
LOCUS       DASPGK010000286         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_309448, whole genome shotgun sequence.
ACCESSION   DASPGK010000286 DASPGK010000000
VERSION     DASPGK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309448"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VEC56_07880"
     CDS             complement(<1..1258)
                     /locus_tag="VEC56_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV14110.1"
                     /translation="MKTRMAIIATVVFLSVGWAALMAGCGKGEGEQGQEATAVQYHCP
                     MHPTVVSDTPGDCPICGMKLVPIEEADDDATTSSDQPASTVPGQAVVSITPKSRQTMG
                     LELGTVERRVLAREVHTSARIVADETRLHHVTVKVDGWVNELYASITGQAVKHGEPLL
                     TIYSPDLLSAQQEYITALKTLEKVSASSDADARQGAADLVTAAHRRLELWDVSEEQIA
                     RLDRTREVEKYVTLYSPMSGVIIERNISAGHNLMAGEVLMTIADLTRVWGDADIYQSD
                     LPYIKVGMPLQMSLPYWRNKTFKGTVIFVSPTLDPATRTLHARLEIPNPELLLRPGMY
                     GDASLSYSLGEKLGIPAEAIMFSGEHTYAFKDGGDGHLIPTRIQVGARGDGWYEVIDG
                     LKEGDRIVVSANFLVDSESSLKAALEA"
     gene            complement(1264..2511)
                     /locus_tag="VEC56_07885"
     CDS             complement(1264..2511)
                     /locus_tag="VEC56_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV14111.1"
                     /translation="MQHLIVWILFAFAAVSPAAAFAADAGTAAPSTLEEYLAIARANN
                     ASVASIVAGARASRERVGVAKGYPDPRLLYGYYVSPDVLEGRQEIILQQEIPFPGKRG
                     LRGEIASREASMAERMSDAMVLEVDFEVKMAFYQYLGLTEIARVLGNEADLLRRMRDV
                     AKVRYESGSAEQQDVLKVELTLSRLADETTINRRDIATTRARLNELIGRDAASPLPDP
                     KWSSPDVSMIDRAALSDSALVHRPEIDGARQEMAAAEATRRLAKREFIPDFMLGVNYE
                     FGAGEDDWWELMAGINLPIWIGKRRAMVREAEAMQKSAEYRMREETLRTLSEVEAATV
                     RARAARERLERFENAILPQAEAAFASSEASYRTGRVDFLDYLDSERMLLEMRRDYAMV
                     RADFGMQLAALERAVGLGSVTRE"
     gene            2855..>3121
                     /locus_tag="VEC56_07890"
     CDS             2855..>3121
                     /locus_tag="VEC56_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXV14112.1"
                     /translation="MKRQRRFAVGIGVILAALGYFAFVGYQEGKAYYKTIDEVAAMGD
                     DAVDKRLRVAGVVQPGSIEREGKELTFTLTQVADDAPRTMVVAYT"
BASE COUNT          499 a         1038 c         1001 g          583 t
ORIGIN      
        1 tcgcttccag cgcggccttc aggctcgatt cgctgtcgac gaggaagttg gcggacacga
       61 cgatgcggtc gccttccttc agcccgtcga tcacctcgta ccagccgtcg ccgcgcgcgc
      121 ccacctggat ccgggtcgga atcagatggc cgtcgccgcc gtccttgaac gcgtaggtgt
      181 gctcgccgct gaacatgatc gcctcggccg ggatccctag cttttcgccg agcgagtagg
      241 agagcgacgc gtcgccgtac atgccgggcc gcagcagcag ctcggggttg ggtatctcga
      301 gccgcgcgtg cagggtgcgc gtcgcggggt cgagcgtggg cgaaacgaag atgacggtgc
      361 ctttgaacgt cttgttccgc cagtaaggca gcgacatctg gagcggcatg ccgaccttga
      421 tgtacggcag atcggactga tagatgtccg cgtcgcccca cacgcgggtg aggtcggcga
      481 tcgtcatgag cacttccccg gccatcagat tgtggccggc gctgatgttg cgctcgatga
      541 tgacgccact catgggcgag tagagcgtca cgtatttctc cacctcgcgg gtgcggtcga
      601 gccgtgcgat ctgttcctcg gaaacgtccc agagttccag ccgtcggtgg gccgccgtaa
      661 ccaagtcggc ggcgccctgg cgcgcatcgg cgtcgctgct ggccgagacc ttttcgagcg
      721 tcttgagcgc ggtgatgtac tcctgctggg ccgagagcaa gtcggggctg tagatcgtaa
      781 gcagcggttc gccgtgcttg accgcttggc cggtgatcga ggcgtacaat tcgttgaccc
      841 acccgtcgac tttcaccgtc acgtggtgca gccgggtctc atcggcgacg atgcgcgccg
      901 acgtgtggac ctcgcgcgcg agcacccgtc gttctaccgt gcccagttcg agccccatgg
      961 tctgccgcga ctttggtgtg atcgagacga cggcctgtcc gggcacggtg gacgcgggct
     1021 ggtcgctcga cgtcgtggcg tcgtcgtccg cttcctcgat ggggacgagt ttcattccgc
     1081 aaatcgggca atcgccgggc gtgtcggaca ccaccgtcgg gtgcatcggg cagtggtact
     1141 gcaccgccgt cgcttcctgg ccctgctcgc cctccccctt accgcagccg gccatgagcg
     1201 cggcccagcc gacggacaag aacacgaccg tcgcgatgat cgccatgcgc gtcttcattg
     1261 ttttcactcc cgcgtcaccg accccaagcc gaccgcgcgc tccagcgccg ccagctgcat
     1321 tccgaagtcc gctctcacca tcgcgtagtc ccttcgcatt tcgagcagca tgcgttcgct
     1381 gtcgagataa tccaggaagt cgacgcggcc cgtgcggtaa ctcgcttccg acgaggcgaa
     1441 ggccgcctcc gcctgcggca agatcgcgtt ctcgaagcgc tcgagtcgct cgcgggcggc
     1501 gcgcgcgcgc accgtcgccg cctccacctc gctcaacgtg cggagcgtct cctctcgcat
     1561 tcgatactcg gcgctcttct gcatcgcctc cgcttcgcgg accatcgcgc gccgttttcc
     1621 gatccagatc gggaggttga tgccggccat caattcccac caatcgtcct cgccggcgcc
     1681 gaactcgtaa ttgacgccga gcatgaagtc cggaatgaac tctcgcttgg ccaagcggcg
     1741 cgtggcttcg gccgccgcca tctcctggcg cgcgccgtcg atttccggcc ggtgaaccag
     1801 cgcggagtcc gagagcgcgg cccggtcgat catcgagacg tcgggcgaac tccatttggg
     1861 atcgggaagc ggtgaagccg cgtcgcgacc gatcagctcg tttaaacgcg cgcgggtcgt
     1921 ggcgatatca cggcgattga tggtcgtttc gtccgccagc cgcgagagtg tgagctcgac
     1981 cttcagcacg tcctgctgtt ccgcgctccc ggactcgtag cgcaccttgg cgacgtcgcg
     2041 catgcgtcgc aggagatcgg cctcgttccc gagcacgcgc gcgatttcgg tcaacccgag
     2101 atactgatag aaggccatct tcacctcgaa atcgacttcc aggaccatcg cgtccgacat
     2161 gcgctcggcc atcgacgctt cgcgcgaggc gatctcgccc cgcaggccgc gcttgccggg
     2221 aaacgggatc tcctgctgga ggatgatctc ctgacgccct tccagcacat cgggggagac
     2281 gtagtatccg tagaggagcc ggggatcggg gtagcccttc gccaccccaa cgcgctcgcg
     2341 cgacgcacgt gcgccggcga cgatcgacgc gacgctcgcg ttgttcgcgc gcgcgatcgc
     2401 gaggtactcc tcgagcgtcg acggcgcggc ggtgccagcg tcggccgcga acgcagcggc
     2461 gggtgacacg gcggcaaatg cgaaaagtat ccaaacgatg aggtgctgca tggtgcttcg
     2521 ggcgggaccg ctgtcggcgg tctggccgca tccgcaacgc aatcgcgatg ccgcccccgg
     2581 cgcgatcctt gcactcttgt aacacgtttc cttttattgt gttacgtctc tagtcgtttc
     2641 ccgagcgtcc gcgctttcgg caaccacata gagaatattc tccattattg gatgatcgtg
     2701 tccacatact cgggggggct gggagctacc ccgatcgggg ccggcgaaga tacgccgctt
     2761 gccgacggag cttcgttgaa cccccgggcg aacttcgggt atattcagcg cggtgcgggg
     2821 ccgcgcgtaa ccctccatcg gagtgtcatc acatatgaaa agacagagac gattcgccgt
     2881 gggaatcggc gtcatcctcg ccgccctcgg gtacttcgcg ttcgtcggct atcaagaggg
     2941 caaggcctac tacaagacca tcgacgaagt cgccgccatg ggcgacgacg cggtcgacaa
     3001 gcgcttgcgc gtggccggcg ttgtgcagcc gggctcgatc gagcgagagg gcaaggagct
     3061 cacgttcacg ctcacccaag tcgccgacga cgcaccgcgc acgatggtgg tcgcgtacac
     3121 g
//
LOCUS       DASPGK010000287         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1360741, whole genome shotgun sequence.
ACCESSION   DASPGK010000287 DASPGK010000000
VERSION     DASPGK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360741"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VEC56_07895"
     CDS             complement(<1..387)
                     /locus_tag="VEC56_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14113.1"
                     /translation="MNVFRYAMVGSALLATLTSCSTTVLEHEASPNASKQSFISDGLV
                     ILGCTASVDTTNALTISDEMCPALEASLSKEEKAVKVTPWKDARAALGDKARLCLLDV
                     GKTGELSAAQIDSVAAALPKSRYVIVH"
     gene            complement(451..900)
                     /locus_tag="VEC56_07900"
     CDS             complement(451..900)
                     /locus_tag="VEC56_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14114.1"
                     /translation="MGAWLRRNALRLAFAAGASWAVVGLFMEDLFAWVPDNAGRSAIE
                     FAVVMMVALASVGITWMITRWRAGFLVPPAGLSAGFWITRALLIPILVWVLLAVVCGL
                     MALVELRITGDPSDSQYGVSVAWAVVWYPVFLTPIVATVALWRAALR"
     gene            complement(906..2972)
                     /locus_tag="VEC56_07905"
     CDS             complement(906..2972)
                     /locus_tag="VEC56_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXV14115.1"
                     /translation="MNPPIAKKIPKNVTVHEDPRVDEYHWLRERDNPDVVAYLRAENE
                     YADAMLAPTDRLQRLLYDEMLARLNETDSSVPVQRDEYFYYTRTEKDRQYEIFCRRRA
                     TDAIETDAPEEVLLDENALAHGRPYFAVGAREVSPDHRYLAYTVDTTGSESFELFVMD
                     LATRAVVDGPIPGLSYDVEWGNDSHTLFYTILDVQMRPHAVRRHRLGAPAVSDVELYR
                     ESDPAFFVGVEVSRSREYLFIVMESNTTTEMHVARSADAVSDAGPSAFRVVQPRRTEV
                     EYHIAHSGESFFILTNEDAFNFRVFEAPARDPSRAQWREIIAHRPSVKIDAVEAFERH
                     LVVVERENGLKRIRVIDLLTRESREVALDEPVYTVWLEQNPEFHATTLRFSYSSLTTP
                     RSVFDYDMAEGTLVLRKRYDVLGGFDPARYASERLYASAVDGTRVPISLVYRRDTPRD
                     GSAPVLLYGYGAYGVSVEPHFVSNRVSLLDRGVIFAIAHVRGGGELGRAWYEKGKRFY
                     KRNTFTDFIACAEHLIAKGYTRAGGIVCYGGSAGGMLMGAVANLRPDLWRAMVAVVPF
                     VDVLNTMLDHSLPLTVIEFEEWGNPEEKKAYEYIRSYSPYENIQRQDYPAMLVLSGFN
                     DRRVQYWEPAKWVAKLRATKTDANPLLLRTRMGEGHKGASGRYDYLKDVALEYAFILE
                     RVGREG"
     gene            complement(2969..4174)
                     /locus_tag="VEC56_07910"
     CDS             complement(2969..4174)
                     /locus_tag="VEC56_07910"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXV14116.1"
                     /translation="MSLLSHLECSQCGRRYDADALRGLCDDCRRPLLARYDLRAAARA
                     IDRAAVQGRARDVWRFHELQPVRDPDLRLSLGEGGTPLVRASRLATALGFSRDAIFIK
                     DEGPNPTGSFKARGMAVAVSRALELGARVLTAPSAGNAGCALAAYAARAGVEAHVYMP
                     ADVPASFTAQCRAYGARIHLIDGLITDCGRASIEDAARFGRFDLSTLKEPYRLEGKKT
                     LGYELAEQSGWNLPDVIVYPTGGGTGLVGMWKAFDEMEALGWIDARRPRMVSVQASGC
                     APIVRAFDHTEDTAAPWEDASTVADGLRVPAAVGDVLMLRSIRESKGTAIAVTDEDLM
                     NGAETIARAEGIFAAPEGGATVAAYTKLRARGWIREGERTVLFNTGSGLSYAHLWSDA
                     GRARLGGSA"
     gene            complement(4239..4637)
                     /locus_tag="VEC56_07915"
     CDS             complement(4239..4637)
                     /locus_tag="VEC56_07915"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HXV14117.1"
                     /translation="MEINYWAVLVAALSGFAVGALWYGPLLGKQWMAASGVTEADVKS
                     TNFPKIYGLTFLMSLVSAYVLAHVVGQFDATTAMAGAQAGFWLWLGFVITVQITDALF
                     NRGSMRLVSIDAGYRLIWLVVMGVILAVWR"
     gene            4978..>5437
                     /locus_tag="VEC56_07920"
     CDS             4978..>5437
                     /locus_tag="VEC56_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HXV14118.1"
                     /translation="MRFGVPFAVLLAAAASPALAADEMPGEATYLSRVRQLTFEGKRA
                     GEGYFSPDGKRFVFQAEREKDNPFYQIYMLSLETGDVHRASNGTGKTTCAYFDPKHER
                     VIFASTHLDPDAVAKQKMEIENRAAGKQRRYAWDYDETYEIFSTALDGSDP"
BASE COUNT          975 a         1826 c         1763 g          873 t
ORIGIN      
        1 gtggacgatg acgtagcgag acttcggcag tgccgcggcc acgctatcga tctgtgcggc
       61 actcagctct cctgtcttcc cgacatccaa caggcacagg cgcgccttgt ccccgagcgc
      121 cgcacgtgcg tccttccatg gcgtcacctt gacggccttt tcttccttcg acagggaagc
      181 ctcgagcgcg ggacacattt cgtcggaaat ggtgagcgcg ttggtggtgt cgacgctggc
      241 cgtacacccc aggatgacga gaccgtcgct gatgaaggac tgcttcgagg cgttgggact
      301 agcttcgtgc tctaggacgg tggtcgagca ggatgtgagt gtcgcgagta atgccgagcc
      361 gaccatcgcg tagcgaaaca cgttcatggt gtccactcct tgtgcgatga ccactgcagc
      421 gtctgatcat agctccgccg ggctcgtccg tcaacgcagc gccgctcgcc acagcgcgac
      481 ggtcgcgacg ataggcgtca ggaacaccgg gtaccacacc accgcccacg cgaccgatac
      541 gccgtactgc gagtcggaag gatcgcccgt gatgcgaagt tccactagcg ccatcaggcc
      601 gcacacgacc gcgaggagaa cccagacgag aatgggaatc aggagcgcgc gcgtgatcca
      661 gaacccggcc gagaggccgg cgggcggaac cagaaacccc gcccgccaac gcgtgatcat
      721 ccacgtgatg ccgacgctcg cgagtgccac catcatgacg accgcgaact cgatcgcgct
      781 tcgacccgcg ttatccggaa cccacgcgaa cagatcctcc atgaacagcc cgaccaccgc
      841 ccaactcgca cccgccgcga aggccaaccg aagcgcgttg cgccgcagcc aggcacccac
      901 ggttactacc cctcgcgccc cacgcgttcc aatatgaacg cgtattcgag tgcgacgtct
      961 ttcaaatagt cgtagcgccc cgaagcaccc ttgtggcctt cgcccatgcg cgttcgcagc
     1021 aagagcgggt tggcgtcggt cttcgtcgcg cgcagcttcg cgacccattt ggccggctcc
     1081 cagtactgca cgcggcggtc gttgaacccg ctcaagacca acatggcggg gtagtcctgg
     1141 cgttggatgt tctcgtaggg cgagtaggac cggatgtact cgtaggcctt cttttcctcg
     1201 gggttgcccc actcctcgaa ctcgatcacg gtcagcggca gcgagtggtc gagcatcgta
     1261 ttgagcacgt cgacgaaggg tacgacggcg accatcgcgc gccacaggtc gggccgcagg
     1321 ttggcgaccg cccccatcag catgccaccc gcgctcccgc cgtagcacac gatgccaccc
     1381 gcccgcgtgt agcccttcgc gatcaggtgc tcggcgcacg cgatgaagtc ggtgaaggtg
     1441 ttgcgcttgt agaagcgctt gcccttctcg taccacgcgc gccccaactc cccgccgccg
     1501 cgcacgtgcg cgatggcgaa gatgacgccg cggtccaaga ggctcacgcg gttggagacg
     1561 aagtgcggtt cgacgctcac gccgtacgcg ccgtagccgt acagcagcac gggtgcgctc
     1621 ccgtcgcgcg gggtgtcgcg gcggtacacg agcgagatgg gcacgcgggt gccgtcgacc
     1681 gcactcgcgt acaggcgttc gctcgcataa cgcgcggggt cgaagccgcc cagcacgtcg
     1741 tagcgcttgc gcagcaccag ggttccctcc gccatgtcgt agtcgaacac ggagcgcggc
     1801 gtcgtgagcg acgaatagct gaagcgcagc gtggtcgcgt ggaactcggg gttctgctcg
     1861 agccacaccg tgtacacggg ctcgtcgagc gcaacctcgc gcgactcgcg cgtcaacaga
     1921 tcgatgacgc gaatgcgctt caacccgttc tcgcgctcca ccaccacgag gtggcgctcg
     1981 aaggcctcga ccgcgtcgat cttgaccgac ggccggtggg cgatgatctc gcgccactgc
     2041 gcgcgcgagg ggtcgcgcgc cggcgcttcg aacacgcgga agttgaaggc gtcctcgttg
     2101 gtgagaatga agaacgactc cccggagtgc gcgatgtggt actcgacctc ggtccggcgc
     2161 ggttgaacga cccggaacgc cgacggcccc gcgtcggaca cggcgtcggc ggagcgcgca
     2221 acgtgcatct cggtggtggt gttgctctcc atcacgatga agagatactc gcggctgcgc
     2281 gacacttcga cccccacgaa gaaggccgga tccgactcgc ggtacaactc gacgtcggag
     2341 acggccggag ccccgagccg gtggcggcgc accgcgtgcg ggcgcatttg cacgtctaga
     2401 atcgtgtaga agagcgtgtg gctgtcgttg ccccactcga cgtcgtacga aagtccgggg
     2461 atggggccgt ctacgacggc gcgcgtcgcg aggtccatga cgaacagctc gaacgattcg
     2521 gatcccgtgg tgtccacggt gtacgcgagg taccggtgat cggggctcac ctcgcgcgcg
     2581 ccgacggcga agtacggacg gccgtgcgcg agcgcgttct cgtccagcag gacctcctcg
     2641 ggcgcgtcgg tctcgatggc gtcggtcgcg cgcctacgac agaagatctc gtactgccgg
     2701 tccttctcgg tgcgcgtgta gtagaagtac tcgtcgcgct ggaccggcac cgacgagtcg
     2761 gtctcgttca ggcgcgcgag catttcatcg tagaggagtc tctggaggcg gtcggtcggc
     2821 gccagcatcg cgtcggcgta ctcgttttcg gcgcgcaggt aggccacgac gtccgggttg
     2881 tcgcgctcgc gcagccagtg gtactcgtcg acgcgcgggt cctcgtggac ggtgacgttc
     2941 ttgggtatct tcttcgcgat cggcggattc acgcactgcc tcccaggcgc gcccgcccgg
     3001 cgtcgctcca caggtgcgcg tatgacaacc cgctgccggt attgaagagc acggtccgct
     3061 cgccctcgcg aatccaaccg cgcgcgcgca gcttggtgta ggccgcgacg gtcgccccgc
     3121 cttcgggggc ggcgaagata ccttcggcgc gcgcgatcgt ttcggcgccg ttcattagat
     3181 cttcgtcggt gactgcgatg gcggtgccct tcgactcacg aatggagcgc agcatgagca
     3241 catcgccgac ggcggcgggg acgcgcaacc cgtccgccac ggtggaggcg tcctcccacg
     3301 gcgcggcggt atcctccgta tgatcgaacg cgcgcacgat gggcgcgcac ccgctggctt
     3361 ggaccgacac catgcggggg cgacgcgcgt cgatccaccc cagcgcttcc atctcgtcga
     3421 aggccttcca catgccgacc aggcccgtac cgccgccggt cggatacacg attacgtcgg
     3481 gcaagttcca ccccgactgc tccgccagct cgtaccccag cgtcttcttc ccttccaggc
     3541 gatacggctc cttgagcgtc gacaggtcga agcgcccgaa gcgcgccgcg tcctcgatcg
     3601 acgcgcgccc gcaatccgta atgaggccgt cgatcaaatg gatacgcgcc ccgtacgcgc
     3661 ggcactgcgc ggtaaacgac gccgggacgt cggcgggcat gtagacgtgg gcttcgacac
     3721 cggcgcgcgc cgcgtacgcg gctagcgcgc aaccggcgtt ccccgctgag ggcgcggtga
     3781 gcacgcgcgc gcccagctcc agcgcgcgtg agacagcgac cgccatgccg cgcgctttga
     3841 acgacccggt cggattgggg ccttcgtctt tgatgaaaat tgcgtcgcgc gaaaaaccga
     3901 gtgccgttgc gagacgcgag gcacgaacga gcggggtccc gccttcgccg agggataatc
     3961 gcaagtccgg gtcgcgaacc ggttgcagct cgtggaagcg ccagacatcg cgcgcgcggc
     4021 cctggaccgc ggcacggtcg atggcgcggg ccgccgcgcg caagtcgtag cgcgccaaga
     4081 gcggacgacg gcaatcgtcg cacaagccac gcagcgcgtc ggcgtcgtag cggcgcccgc
     4141 actgactgca ttcgagatgc gatagaaggc tcatggtcgt cgatggcgcc cttacgaccg
     4201 ccgacgaagt tcggctcgca acgagatcgg gggtccggct accgccacac cgcgagaatg
     4261 acgcccatca ccaccagcca gatcaagcgg tatccggcat cgatcgaaac caggcgcatg
     4321 ctgccgcgat tgaacaacgc gtcggtaatc tgcaccgtaa tgacaaagcc cagccacagc
     4381 cagaagcccg cttgggcacc cgccatcgcc gtcgttgcat cgaactgccc caccacgtgc
     4441 gcgagcacgt acgcggagac caagctcatg aggaatgtga gaccgtatat cttggggaag
     4501 ttcgtgctct tgacgtcggc ttcggtgacg ccggaagcgg ccatccactg cttgccgagg
     4561 agcggtccgt accataacgc accgaccgcg aagcccgaca acgccgccac gaggacggcc
     4621 cagtagttga tctccatgga gacccccttc ggtatccggg agtggttcgg gaaagcgccg
     4681 ggggaagggt accgggaagt gaacggggag gacaatcacg aaaaaaaagc ggcgccgaaa
     4741 atgatgagac ggcgggcacc ggacccgctt tgaccacgtg cgatagccag ccacagcgtt
     4801 ttgcttccac gggctacccg acacgccgcc gttctcatca gtcgtttcag cgtcgccaca
     4861 tggactacac ccggccggag ggaaagttgg cgggggcttg gcgagtcggc cttcctgggg
     4921 taggatgggg cggcccagcc gactcgtcct acccggattc gatataagga gacgctcatg
     4981 cgttttgggg ttccgttcgc cgtcttgctc gccgccgccg cgagcccagc ccttgccgcc
     5041 gacgaaatgc ccggcgaggc cacgtatctg tcccgcgtcc gccagctcac gttcgaaggc
     5101 aagcgcgcgg gcgaaggcta cttctcgccc gacggcaagc gcttcgtatt ccaggccgag
     5161 cgcgagaagg acaatccgtt ctatcagatc tatatgctct cgctcgaaac cggcgacgtg
     5221 caccgcgcct ccaacggaac cggcaagacg acgtgtgcct acttcgatcc caaacacgag
     5281 cgggtgatct tcgcctccac gcacctggat ccggacgcgg tggcgaagca aaaaatggag
     5341 atcgagaatc gcgcggcggg caagcagcgc cgctacgcct gggactacga cgagacgtac
     5401 gagatcttct cgacggcgct cgacggctct gacccga
//
LOCUS       DASPGK010000288         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1244580, whole genome shotgun sequence.
ACCESSION   DASPGK010000288 DASPGK010000000
VERSION     DASPGK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244580"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VEC56_07925"
     CDS             <1..384
                     /locus_tag="VEC56_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14119.1"
                     /translation="LATTADSAGLSAAIDRPGVFAVFRDEAAPWLGAVKVVAAVSYAT
                     GSTRYEIHVPVDDEGSGFDDARSEVYVGGIKQIWRWDFVGKKMIVALHDDSIIGPQPV
                     RVVAFDRIGNSSSVDATVTIASRAP"
     gene            395..>3125
                     /locus_tag="VEC56_07930"
     CDS             395..>3125
                     /locus_tag="VEC56_07930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXV14120.1"
                     /translation="MPAPFVHLHAHSEFSLLDGSIKVEDLVRRARELDMPALALTDHG
                     SLFGLIHFYKAAKKHGVKPILGMEAYITRGSRHDRGRKKGEPSHTDHLILLARNLEGY
                     HNLIKLSSIAYLEGFYYKPRIDFDLLASHSGGLIGTTACLRGTVAQAALHEGFAQACE
                     TALRYRDIFGEGNFYVEMQDHGLEPQKQLNDIYRRLSKELGLPLTVSNDVHYLYKDDA
                     EAHEVLLCLQTGSDIDDPRRFRFTTNELFFKTPEEMRALFPNDAEALANTVAIAERCD
                     VELREGALHLPRFPLPDGFATNDDYLKHLAYEGIARRYRETTDTMRQRVDFELSVIAK
                     MGFAGYFLIVRDIVDHARAKGIPVGPGRGSAAGSLVTYAVGITDVDPLAHGLLFERML
                     NPERVSMPDIDIDFCFERRDEVIRYVIDRYGQDNVCQIITFGTMAAKAVVRDVGRVLK
                     IPYDEVDRLAKLIPGTPGTSLKESIENIGDLKTLVDNSPDYRRLMKLSLTLEGLSRHA
                     STHAAGMIITPTPLVNHVPLYKSNRDEITTQYDMKSIDAIGLLKIDVLGLRTLTVIDK
                     ALRMVEENHGRRLAVEDIPMEDEKTYELLRAGRTVGVFQLESEGMRELLKNLKPTAFH
                     DVVAVNALYRPGPLGSGMVTTFVDCKHGRKEIVYELDILKPILADTYGVILYQEQVMR
                     SASDLAGFTLGEADLLRKAMGKKQKDVMAEQRKKFVEGARARGTHKAIAEKIFTQMEK
                     FAEYGFNKSHSAAYAVLSIRTAYLKAHYPAEFMAATLSSEIDDTDRITVLLDDCREMA
                     IDVVPPNINTCDPDFRARDGRIFYGLGAVKNVGLSAVETLVAERNEGGAFRSLENVCS
                     RLNSRLITKRVYESLIAAGAMDTLPGHRAQKAAALETIMERAARSFRDAERGQF"
BASE COUNT          594 a         1030 c         1001 g          500 t
ORIGIN      
        1 ctggccacga ccgcggattc ggccggcctg tcggccgcca tcgaccggcc gggcgtcttc
       61 gccgtgtttc gcgacgaggc cgcaccctgg ctcggcgccg taaaggtcgt ggcggccgtg
      121 tcctacgcca ccgggtccac ccgatacgag atccacgtgc cggtcgacga cgagggctcc
      181 ggtttcgacg acgcgcgctc ggaggtatac gtgggtggaa tcaaacaaat ttggcgctgg
      241 gacttcgtgg gcaaaaaaat gattgtggcg ctgcacgacg attctataat cgggccgcaa
      301 cccgtccgcg tcgtcgcctt cgatcgtatc ggcaattcgt cgagcgtcga cgccaccgtc
      361 accatcgcat cgcgcgcgcc gtgagcaggt caccatgccc gcaccgttcg ttcatctgca
      421 cgcccactcc gagttcagcc tgctcgacgg gtcgatcaag gtagaagacc tggtgcgccg
      481 cgcgcgcgag ctcgacatgc cggcgctcgc gctgaccgac cacggcagcc tcttcgggct
      541 cattcacttc tacaaggcgg ccaagaagca cggggtcaag ccgatcctgg gcatggaggc
      601 gtacatcacg cgcgggagcc gtcacgaccg cgggcgcaag aagggcgagc cctcgcacac
      661 cgatcacttg attctcctcg cgcgcaacct cgaggggtat cacaacctga tcaagctctc
      721 gtcgatcgcg tatctggagg gtttctacta caaaccgcgc atcgacttcg acctgctggc
      781 gtcgcactcg ggcggcctca tcggcaccac cgcgtgcctg cgcggtacgg tcgcgcaggc
      841 cgccctgcac gagggtttcg cccaggcgtg cgagacggca ctccgctacc gcgacatctt
      901 cggcgaaggg aatttctacg tcgagatgca ggaccacggc ctcgagccgc agaagcagtt
      961 gaacgacatc taccgcagac tgtcgaagga gctcgggctc ccgctgacgg tgtccaacga
     1021 cgtgcactat ctatacaagg acgacgcgga ggcgcacgag gtgctcctgt gcctgcaaac
     1081 cgggagcgac atcgacgacc cgcgccgctt ccgcttcacc accaacgagc tcttcttcaa
     1141 gacgcccgaa gagatgcgcg cgctgttccc gaacgacgcg gaagcgctcg ccaacacggt
     1201 cgccatcgcg gagcgctgcg acgtcgagct gcgcgagggg gctctccacc tcccgcgctt
     1261 cccgctgccc gatggtttcg cgaccaacga cgactacctg aagcacctcg cctacgaggg
     1321 gatcgcacgc cgctaccgcg agaccaccga caccatgcga cagcgcgtcg acttcgagct
     1381 ctcggtgatc gcgaagatgg gctttgcggg gtacttcctg atcgtgcgcg acatcgtgga
     1441 ccacgcgcgc gcgaagggca tcccggtggg gccggggcgc gggagcgccg cggggagcct
     1501 ggtcacttac gcggtgggga tcaccgacgt cgacccgctc gcgcacgggc tcctgttcga
     1561 gcgcatgctc aacccggagc gcgtgagcat gcccgacatc gacatcgact tttgcttcga
     1621 gcggcgcgac gaagtgattc gctacgtgat cgaccgctac gggcaggaca acgtgtgtca
     1681 aattattacc ttcggaacca tggccgccaa ggcggtggtg cgcgacgtcg ggcgcgtgct
     1741 caagatcccc tacgacgaag tggaccgcct ggccaagctc atcccgggca cgccggggac
     1801 gtcgctcaag gagtcgatcg agaacatcgg agacttgaag acgctcgtcg acaacagccc
     1861 cgactaccgg cgcttgatga agctctcgct cacgttggag ggtctttcgc gccatgcgtc
     1921 cacccacgcg gccggcatga tcatcacgcc cacaccgctg gtcaatcacg tgccgctcta
     1981 caagtcgaac cgcgacgaaa tcacgaccca gtacgacatg aagagcatcg acgcaatcgg
     2041 tctcttgaag atcgacgtgc tggggttgcg cacgctgacc gtgatcgaca aggcgctgcg
     2101 catggtggag gagaaccacg ggcgccggct ggccgtggaa gacatcccca tggaagacga
     2161 gaagacgtac gagctcttgc gcgcggggcg tacggtgggt gtgttccagc tcgaaagcga
     2221 aggcatgcgc gagctcttga agaacttgaa gcccaccgcg ttccacgacg tggtcgcggt
     2281 caacgcgctc taccgccccg gtccattggg aagcggcatg gtgacgacct tcgtcgactg
     2341 caagcacggt cgcaaagaga tcgtttacga gctggacatc ctgaagccga ttctggccga
     2401 cacctacggc gtcatcttgt accaggagca ggtgatgcgc agcgcgagcg acctggccgg
     2461 cttcaccctg ggggaggcgg acctcctgcg caaggcgatg gggaagaagc agaaggacgt
     2521 catggccgag cagcgcaaga agttcgtcga aggagcgcgc gcacggggga cgcacaaggc
     2581 gatcgccgag aagatcttca cccagatgga gaagttcgcc gagtacggtt tcaacaagtc
     2641 gcactccgcg gcctacgccg tactctcgat tcgcaccgcc tacctgaagg cgcattaccc
     2701 ggcggagttc atggccgcca ccctgtcgag cgagatcgac gacaccgacc gtatcacggt
     2761 gctgctcgac gactgccgcg agatggcgat cgacgtggtg ccgcccaaca tcaacacgtg
     2821 cgaccccgat tttcgcgcgc gcgacggccg catcttctac ggcctgggcg cggtcaagaa
     2881 cgtgggtctg tcggcggtcg agaccctggt ggcggagcgc aacgaggggg gtgcctttcg
     2941 ctcgctggag aacgtgtgca gccgcctgaa ctcgcgcttg atcaccaagc gcgtgtacga
     3001 aagcctgatc gcggccggtg ccatggacac gctgcccggc caccgcgcgc aaaaggcggc
     3061 cgcgctcgag accatcatgg agcgcgcggc gcgcagcttc cgcgacgccg agcgcgggca
     3121 gttcg
//
LOCUS       DASPGK010000289        11308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_425888, whole genome shotgun sequence.
ACCESSION   DASPGK010000289 DASPGK010000000
VERSION     DASPGK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425888"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VEC56_07935"
     CDS             complement(<1..197)
                     /locus_tag="VEC56_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV14121.1"
                     /translation="MKSRTVSVTIRRTPKEVYDFAVEVANFPRWATSFCRSAQPDGDD
                     WLLDTPLGAVRLRFVPRNAFGV"
     gene            complement(243..806)
                     /locus_tag="VEC56_07940"
     CDS             complement(243..806)
                     /locus_tag="VEC56_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14122.1"
                     /translation="MVTVMSLWLPILVSAVVVFIASSIVHMVLKYHANDFAKIPNEDA
                     IMDALRKFNIPQGEYCVPRADSMKAMKEQAFVDKLNKGPVLLMTILPNGPFNMGKSLG
                     LWFGHSVIVSVFAAYIAGRALGPGSDYLDVFRFAGTVAFVGYTLGVWQGWIWYKRKTS
                     TVIKLTIDGLVYGLCTGGVFGAMWPGM"
     gene            complement(912..1805)
                     /locus_tag="VEC56_07945"
     CDS             complement(912..1805)
                     /locus_tag="VEC56_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14123.1"
                     /translation="MMDLSCQPGSARRLILTVFAILLSALDVRGAHGHIAIYSDEGAT
                     ECTVELPALNETVRIYVVMDPYPGKSAVQFSLPMPACSPYDVAGWTLSPGYVSQGDLA
                     SGVQVAFVGCELDDIVVMSVDLVRTRDPQAACCRIKLGPHTTLGFSSSVEAFDCGGGG
                     SPLPIDDYPARLSGDESCVQLPPPSDPFPADGATGVLLDVELGCTLHPAQYWCGPFPL
                     GSDWVRIYLGTDANPPLVGNGGPFPFPTEMLAPATTYYWRVDYSYWSIGPISSPLWNF
                     TTTAATAVETSTWGAIKALYR"
     gene            complement(1862..3151)
                     /gene="purB"
                     /locus_tag="VEC56_07950"
     CDS             complement(1862..3151)
                     /gene="purB"
                     /locus_tag="VEC56_07950"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXV14124.1"
                     /translation="MIERYTLPEMGRIWTEQNKLDKWLQFEILACEALAQLGEIPHDA
                     VERIKKHARFDAARVQEIEATTHHDVIAFLTNVAEHIGNDSRYVHLGLTSSDLLDTTL
                     AAQIKEAGELLLRRIGDLRGAIKARAIEHKKTVMVGRTHGIHAEPITFGLKLLVWYDE
                     LGRREHTLRAAIEAVSVGKVSGSVGTFAHASPKVEEYVCKKMGLKPAPVSTQVVQRDV
                     HAEFMSALALVAASLEQFATEVRNLQRSDVLEVEEPFRKGQKGSSSMPHKRNPIICER
                     VVGLSRLMRGYALAAMENVALWHERDISHSSVERVILPDATITLDYMLVKFTAVVKDL
                     VVYPENMRRNMDKVHGLIYSQKLLLELARAGLSREDAYALVQEAAMETWETGKPFEET
                     VRARKGITAKVDAKALAAVFNLDDYLKEVDTIFARVR"
     gene            complement(3161..4363)
                     /locus_tag="VEC56_07955"
     CDS             complement(3161..4363)
                     /locus_tag="VEC56_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV14125.1"
                     /translation="MYDGSERLPRKIRFLPFVFGGLTGVSIALVVIAFVLVQRNTDGR
                     AESLLQTGSTYAAGTGASIDDDRRNAIVAATEQVAPAVVSITASYTRRTRPVYDIWFN
                     RYYPGRERTLAELGSGVIVDKRGYVFTNYHVIANAERLQVTLSDGKTYAGTLVGSAPS
                     YDLALIKIDGEDFPTAPLGNSDDLLVGEWAIAIGSPFGFSLLDTQPTVTVGVISANHR
                     DIKQEEDSQQIFNDMIQTDAAINPGNSGGPLVNARGQVIGINTLIFSPTQGTYAGNVG
                     MGFAIPINRARRVMEEIVQYGRVRDVWLGMSATDINAETQAALNLPTASGVLVQSIEE
                     GGPADKAGLKVGDQIIAINNVALETRFQANRVIFGSGVGDVLEMKINRKDKLIDMKIT
                     LEERPSDI"
     gene            complement(4350..5021)
                     /gene="fsa"
                     /locus_tag="VEC56_07960"
     CDS             complement(4350..5021)
                     /gene="fsa"
                     /locus_tag="VEC56_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HXV14126.1"
                     /translation="MKFFLDTANLDEIREAAALGVLDGVTTNPSLMAKEDGDDYSAVL
                     KEICDIVGGPVSAEVVADESGEMVRQGRAFARLSEHIVVKIPMTPEGMKAVKALSADD
                     IKTNVTLCFSANQGLIAAKAGATYISPFIGRLDDAGMDGMQVVADLVDIYERYAFPTQ
                     VLAASIRHPMHVVEAARLGADVSTMPYKIFQQLFKHPLTDVGIELFNRDWAKVMERRK
                     TNVRR"
     gene            complement(5073..5840)
                     /gene="truA"
                     /locus_tag="VEC56_07965"
     CDS             complement(5073..5840)
                     /gene="truA"
                     /locus_tag="VEC56_07965"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXV14127.1"
                     /translation="MRNIRLSLEYDGTDFQGWQKQPGARTVQGTLETAIQIVCRHPAE
                     TIGCARTDAGVHALGYVANFQTESDIASSRLSLALNGCLPEDVAVIAAVDTPADFHAR
                     FSARSRRYLYRITTVPTAVWRRYAFHSHHALDVDRMAAAGTLLVGEHDFTSFTPVNNE
                     AEKRCRVIDLAVAREGPHITISVEATRFLHHMVRVIAGTLMEVGRGRMEPEQVGEALR
                     KKDRRAAGPTAAAQGLVLLRVRYDDGPGRELPGLGKP"
     gene            complement(5847..6614)
                     /locus_tag="VEC56_07970"
     CDS             complement(5847..6614)
                     /locus_tag="VEC56_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HXV14128.1"
                     /translation="MDHRSHDPSPARSRPRDPRAAFLVMLLVVVASLVTIRDAWAMLA
                     PLAFVCAWHGWVARRPRATARVLVRVLPFAAFIIVLNAVLVPGEALVSVAGRRVVSRE
                     GLADGVFFALRLAVMLVAVASFLAASAPESMARGVYDVLRRFSQRAASRVALLVFLSM
                     GFVPLLSDELRRIRVAQFFRGGAFSGGLVRRAEIARSWLVPLLVSAIHRSGELAKSVE
                     LRGIRERLVHTIEPPRLHLADVVLVAVAIAVVAWASA"
     gene            6715..8706
                     /locus_tag="VEC56_07975"
     CDS             6715..8706
                     /locus_tag="VEC56_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV14129.1"
                     /translation="MITHRLFMGTTLLACALALGANSNSRAASRYAPLMASSAGQDTL
                     RNLALWEDQRVTGNGKIYDYLQNGSPLVRRRAVEALGRMQEPSDAARLIPLLKERDSE
                     VLRETIFALGQIGNREAVAPLLAARSGALPDVAGLIAEALGKLGGDEAIAALDAMQRD
                     FATSVRADAALALARTPDPNAANALLLAVHDPDPSVMWRAAYALEKQTPMPRSCTAIQ
                     PLLENDDPLVRAYAVRSLGKLQCKQAGKPLASLLKDADLRVVVNAARALGEIKDKGAV
                     HPLAQIIADHESHHARAAAAEALGKIDDENGKDALAQGLLDTSAMVRINSIRALAEVL
                     GENAEMFCDQMRRDGSRLVRAEAIRSYGTAGLAKRASQIDEIARTDKDPLMRAAAIEA
                     LAKLKDAKVAPLLVPALRDADFTVATAAVVAIGDQQYKAAVPALMDAYRARPEREFVD
                     VQLEAVRVLGDLGALESEALLVEATSHDDARVRAAAVASLKKLGLTAPELPSERTFHE
                     QAFDPARKKQLAPPAGTRHAVIATRHGAIEIELFGDDAIQTVANFIHLAKSGFYKKLT
                     THRVVPNFVVQGGDPRGDGSGDAGYTVPAEVSRHHFGEGFLGIADAGKDTGSCQWFIT
                     LSPQRHLDGRYTIFGRVTKGMDVVWKIDQGDAFDVKILD"
     gene            complement(8747..9244)
                     /locus_tag="VEC56_07980"
     CDS             complement(8747..9244)
                     /locus_tag="VEC56_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXV14130.1"
                     /translation="MSQDALFCARCGGPMAPRRVDDRVRAVCDRCGFVAYRNPAPAAG
                     VVLVEAGEVLMVQRKFEPRVGMWTLPAGFVEYDEHVTECAIRETKEETNLDVELDGLF
                     GAYMAMDDPRVRAVLLLYRCRRIGGDLRAGDDALDARFFPLDRTPKEIAFKAHAQALD
                     DLRKG"
     gene            complement(9241..10527)
                     /locus_tag="VEC56_07985"
     CDS             complement(9241..10527)
                     /locus_tag="VEC56_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14131.1"
                     /translation="MMQTTRLRRTGYAGAALLVAAAALMASCGKGKPDYDTNLLVNAS
                     FEDVGRDGIPRGWSIENFRGPENEFAIRYGVDDQIAQDGKRSWFFAGDPNTKRFFLLS
                     QEVEVRDATHVRLRGWIQTDNVDLKSADQLTHCNFLLTFYDENHHRFQELRVSDKRTR
                     PVVDTRLWAEDDVKFRLPQGTRFVKVSCVLAMTGQAWFDNVSLEVPTPIDWQTATTKN
                     FVFHWLPGHPMPDGSQAAQQMIWDTIAKKLGLESNVVVQYYFYPDTTTIRSMLSLKGY
                     QYVSWDDVEFHSINANDNHEIVHFMTDPIGRAPRALAEGTVFWLHDDVDGLPVEEAMK
                     KLVRANLVPPLRSMFEYNLFMSIEPPISMPAAASYVGFLVDRFGVEKLMELYKAANGM
                     TAYEGVAQATEKVYGIPLSEIEAAWLTRQRLRASQG"
     gene            complement(10524..>11308)
                     /locus_tag="VEC56_07990"
     CDS             complement(10524..>11308)
                     /locus_tag="VEC56_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14132.1"
                     /translation="RSEGSPRCSGRRVRWAIAIGLATCAAASLHAAPPMRATKEASVV
                     STAHARYAILQGDQEIGTESVTRRVFDNNTIRFDAENLITNPGVAMTTRSELVIEEES
                     YFPRSYRADKTVRQSDDEFVHALTVDMVANVAVLGSELRGAKNSRRVVVPAGTAIQEV
                     GTVHPWYQLLFWVDPASEGRQRIQWLDPASGVLDAGELYVAGQETIAVLGKKTRVTVY
                     KAERERLGEATLYVDAKKRIVRAEQNMLIYRLDQWSEELAKK"
BASE COUNT         1954 a         3802 c         3663 g         1889 t
ORIGIN      
        1 acaccgaacg cgttgcgcgg cacgaagcgg aggcgcaccg cgcccagggg agtatcgagc
       61 agccaatcgt cgccgtccgg ctgggccgag cggcaaaacg acgtcgccca gcgcgggaag
      121 ttggcgacct cgaccgcgaa gtcgtagacc tctttcggcg tccggcggat ggtgacgctg
      181 accgttcgtg acttcacggt ctactcatga ccgcttcgcg ccgcgcccga ctgtgacggc
      241 gattacattc ccggccacat cgcgccgaac acgccgcccg tgcacaggcc gtagaccaga
      301 ccgtcgatgg tcagcttgat caccgtgctc gtcttgcgct tgtaccagat ccagccctgc
      361 cacaccccca gcgtgtaccc cacgaacgcg accgtccccg cgaagcggaa cacgtcgagg
      421 tagtcgctcc ccggacccag cgcgcgcccc gcgatgtagg ccgcgaatac gctcacgata
      481 acgctgtggc cgaaccaaag ccccaggctc ttccccatat tgaacgggcc gttgggcaat
      541 atcgtcatca acaagaccgg acccttgttg agtttgtcga cgaacgcctg ctccttcatc
      601 gctttcatgc tgtccgcacg cgggacgcaa tactcgccct gcgggatgtt gaacttgcgc
      661 agcgcgtcca tgatcgcgtc ttcgttggga atctttgcga agtcgttcgc gtggtacttg
      721 agcaccatat ggacgatcga gctggcgatg aagaccacga ctgccgagac gagaatcggg
      781 agccagagag acatcacggt gaccatggcg acctccttga aagatgtctc gcgtgagggc
      841 gcgagtgcgg aggattctag ccggacgcgt ggaagggcgg aagatgtttt cgccgatacc
      901 gagcgaaacc gctatctata taacgctttg atcgcgcccc aggtcgaggt ttccacagct
      961 gtcgcagcgg tcgtggtgaa gttccatagc ggtgaggaaa tgggcccgat actccagtac
     1021 gaatagtcga cgcgccagta atacgtggtc gccggtgcca acatctccgt tgggaacggg
     1081 aaaggcccac cgttcccgac gagcggtgga tttgcatcgg tgccgagata gattcgcacc
     1141 cagtcgctgc cgagcggaaa tgggccgcac caatactggg ccggatgcaa cgtgcagcct
     1201 aactcgacgt cgagcaacac tcccgtggca ccatccgcgg gaaacgggtc cgacggcggg
     1261 gggagttgca cgcacgattc gtcgccggaa agccgcgctg gatagtcgtc gatggggagc
     1321 ggcgacccac cgccaccgca atcgaaggcc tcgacgctac tgctgaaccc aagggtcgtg
     1381 tgcggcccaa gctttatccg gcagcaagcg gcttggggat cgcgggttcg aacaaggtcg
     1441 acgctcataa ccacgatgtc gtcgagctcg cacccgacga acgcgacttg gactcccgac
     1501 gcgaggtcac cttgcgacac ataacccggc gagagcgtcc atcccgccac gtcgtacggc
     1561 gaacaagcgg gcattgggag actaaactgc acggcggact ttcccgggta cgggtccatc
     1621 acaacgtaga tgcggacagt ttcgttgagc gcaggaagct cgacggtgca ctcggtcgca
     1681 ccctcgtcgc tatagatagc gatatggccg tgtgcgccgc gaacatccag cgcggataga
     1741 aggatcgcga acacggtaag gatgagccgt cgcgccgatc ctggttggca gctgagatcc
     1801 atcatcgcaa tcaccggcca attgagagtt ggaatcgcga tgtattatac cacgccggga
     1861 actagcggac tcgcgcgaag atggtgtcga cctccttgag atagtcgtcc aggttgaaga
     1921 cggccgccag cgccttggcg tccactttgg ccgtgatgcc cttgcgcgcg cgcaccgtct
     1981 cttcgaaggg tttgccggtc tcccacgtct ccatggccgc ttcctgcacc agcgcgtagg
     2041 cgtcctcgcg cgagagcccg gcgcgggcca attccaacaa aagcttctgc gagtagatca
     2101 acccgtgcac cttgtccatg ttgcggcgca tgttctcggg atagacgacc aggtccttaa
     2161 cgaccgcggt gaatttgacg agcatataat cgagggtgat ggtggcgtcg ggcaggatta
     2221 cgcgctccac cgacgagtgc gagatgtcgc gctcgtgcca cagcgccacg ttctccatcg
     2281 cggccagcgc gtagccgcgc atcagtcggg agagccccac gacgcgctca caaatgatgg
     2341 ggttgcgctt gtggggcatc gacgagctgc ccttctgccc cttcctgaac ggctcctcga
     2401 cttcgagcac gtcgctgcgc tggaggttgc gcacctcggt ggcgaactgc tccaacgaag
     2461 cggccaccag cgcgagcgcc gacatgaact cggcgtgcac gtcgcgctgg acgacctggg
     2521 tcgaaaccgg cgcgggcttg agccccatct tcttgcacac gtactcctcg accttgggac
     2581 tcgcgtgggc gaaggtcccc accgaccccg acaccttccc caccgagacc gcctcgatcg
     2641 cggcgcgcag cgtgtgctcg cggcgaccga gttcgtcgta ccacaccagc agcttcaggc
     2701 cgaaggtgat cggctcggcg tggatgccgt gcgtgcgccc gaccatgacg gtcttcttgt
     2761 gctcgattgc acgcgccttg atcgctccgc gcaagtcgcc gatgcggcgc agcaagagct
     2821 cgcccgcctc cttgatctgg gccgcgagag tggtgtcgag caggtcggaa gacgtgagcc
     2881 cgaggtggac gtagcgcgag tcgttgccga tgtgctcggc gacgttggtc agaaacgcga
     2941 tgacgtcgtg gtgggtggtt gcttcgatct cttggacgcg ggccgcgtcg aagcgcgcgt
     3001 gcttcttgat gcgctcgacc gcgtcatggg gtatttcgcc gagctgcgcc agggcttcgc
     3061 aggcgaggat ctcgaactgg agccacttat cgagcttgtt ctgctcggtc cagatgcggc
     3121 ccatttcggg gagggtgtat cgttcaatca tacgggcatt ctaaatgtcc gacggccttt
     3181 cttcgagcgt gatcttcatg tcgatcagct tgtccttgcg attgatcttc atctccagca
     3241 cgtcgcccac acccgagccg aagatgacgc ggttggcctg gaagcgcgtc tccagcgcaa
     3301 cattattgat cgcgatgatc tggtcgccca ccttcaggcc cgccttgtcg gccggcccgc
     3361 cctcttcgat gctctgcacc agcacgccgc tcgcggtggg caaattcaac gcggcctgcg
     3421 tctccgcgtt gatgtcggtc gccgacatcc ccagccacac gtcgcgcacg cgaccgtact
     3481 ggacgatctc ctccatcaca cggcgcgccc ggttgatggg aatcgcgaag cccatgccga
     3541 cgttgccggc gtaggtgcct tgggtgggag agaagatcag cgtgttgatg ccgatcacct
     3601 ggccgcgcgc gttgaccagc ggcccgccgc tgttgccggg attgatggcg gcgtcggtct
     3661 ggatcatgtc gttgaagatc tgctggctgt cctcttcctg cttgatgtcg cggtggttcg
     3721 cactaataac gccgacggtc acggtcggct gggtatcgag caggctgaag ccgaacggac
     3781 tcccgatcgc gatggcccac tcgcccacca ggaggtcgtc ggagtttccc agcggcgcgg
     3841 tcgggaaatc ttcaccgtcg atcttgatca gtgccaagtc gtagctgggg gcactcccca
     3901 ccagcgtgcc cgcgtaggtc ttgccgtcgg acagcgtcac ctgcaggcgc tcggcgttgg
     3961 cgatcacgtg gtagttcgtg aacacgtagc cgcgcttgtc gacgatgacg cccgagccca
     4021 actcggccag cgtgcgctcg cggcccgggt agtagcggtt gaaccagatg tcgtacacgg
     4081 gtcgcgtgcg gcgcgtgtag gacgcggtga tgctcaccac ggcgggagcc acctgttcgg
     4141 tcgcggccac gatggcgtta cggcggtcgt cgtcgatgga cgcgcccgtc cccgccgcgt
     4201 acgtcgagcc ggtctgcagc aacgactcgg cgcggccgtc ggtgttgcgt tgcaccagca
     4261 cgaacgcgat cacgaccagc gcgatcgata cgcccgtcag cccgccgaac acgaagggta
     4321 gaaaccgaat cttgcgcggg agtctttcgc taccgtcgta cattcgtctt gcgtcgctcc
     4381 atgaccttgg cccagtcgcg gttgaacagc tcgattccga cgtcggtcag cgggtgcttg
     4441 aagagctgct gaaagatctt gtacggcatc gtcgacacgt cggcgcccag gcgggcggct
     4501 tcgaccacgt gcatcgggtg gcgaatgctg gccgcaagca cctgggtcgg aaacgcgtag
     4561 cgctcgtaga tgtcgaccaa atccgccacc acttgcatgc cgtccatgcc ggcgtcgtcc
     4621 aagcgcccga taaaggggct gatgtaggtg gcacccgcct tggccgcgat caagccttgg
     4681 ttcgcgctga agcacagcgt cacgttggtc ttgatgtcgt cggccgagag cgccttgacc
     4741 gccttcatgc cttccggcgt catcgggatc ttgaccacga tgtgctcgct caagcgcgcg
     4801 aaggcgcgtc cctggcgcac catttcgccg ctctcgtcgg ccaccacttc cgcgctcacc
     4861 ggaccgccca cgatgtcgca gatctctttt agaacggccg agtagtcgtc gccgtcctcc
     4921 ttcgccatga gcgaggggtt ggtggtgaca ccgtccagaa cgcccagggc cgcggcttcg
     4981 cggatctcgt cgaggttcgc cgtatcgaga aagaatttca tgccggtcgg tcctttcgcg
     5041 gatgcccgca acggggttag ggccccatca tatcacggct tacccaggcc cggaagctct
     5101 ctacccgggc cgtcgtcgta gcgcacgcgc aggagcacga gcccctgcgc ggcggcggtc
     5161 ggcccggccg cccggcggtc ctttttacgc agcgcctccc ccacctgttc cggctccatg
     5221 cgcccccgtc ccacttccat cagggtgccc gcgatcacgc gcaccatgtg gtggaggaag
     5281 cgcgtggcct cgacgctgat ggtaatgtgc ggcccctcgc gcgcgaccgc cagatcgatc
     5341 acgcggcacc tcttctcggc ctcgttgttg acgggcgtga agctggtgaa gtcgtgctcg
     5401 cccacgagca gcgttcccgc cgccgccatg cggtcgacgt cgagcgcgtg gtgggaatgg
     5461 aacgcgtagc ggcgccagac cgcggtaggg accgtcgtga tgcgatagag gtagcggcgc
     5521 gagcgcgccg agaagcgggc gtggaaatcc gccggcgtgt cgacggcggc gatgaccgcg
     5581 acgtcttcgg gcaggcaccc gttgagcgcg agcgagagcc gcgacgaagc gatgtcactc
     5641 tcggtttgaa aattggcgac gtagcccagc gcgtgcacgc ccgcgtcggt gcgggcgcac
     5701 ccgatggtct ccgccgggtg acggcacacg atctggatcg cggtttccag ggtgccctgc
     5761 accgtgcgcg caccgggctg cttctgccaa ccctggaaat cggtaccgtc gtactcgagg
     5821 ctcaggcgga tgttacgcat gcgctttcac gccgacgccc acgcgacgac cgcgatcgcg
     5881 acggccacca acacgacgtc ggcgaggtgg aggcgcggcg gctcgatggt gtgcaccaag
     5941 cgctcgcgaa tgccccgcaa ctcgaccgac ttcgcgagct cgccggagcg gtggatggcc
     6001 gagacgagca gcgggaccag ccacgagcgc gcgatctccg cgcgccgcac gagtccgccc
     6061 gagaacgcgc cgccccggaa gaactgtgcc acgcgaatgc ggcgcagttc gtcggaaagg
     6121 agcgggacga agcccatcga caagaaaacg aggagcgcca cgcgcgaggc cgcgcgctgc
     6181 gaaaagcgcc gaagcacgtc gtagacaccg cgcgccatcg actcgggcgc ggacgccgcc
     6241 aggaaagacg ccaccgcgac cagcatcacg gccaggcgca gcgcgaagaa caccccgtcg
     6301 gcgagtcctt cccgcgagac gacacgacga cccgccaccg aaaccagcgc ctcgccggga
     6361 acgaggacgg cgttgagcac gatgatgaac gccgcgaagg gcaagacgcg cacgaggacg
     6421 cgcgcggtgg cgcgcgggcg ccgcgcgacc cacccgtgcc acgcgcacac gaaggcgagt
     6481 ggtgcgagca tggcccaggc gtcgcggatg gtaacgagcg aagccaccac cacgaggagc
     6541 atcaccagga atgcggcgcg cgggtcgcgg gggcgcgatc gcgccggcga ggggtcatgc
     6601 gagcggtgat ccatgcccca ggataccccg gccgcatgcg ccgcacaacc gccgcgggcc
     6661 ggggccgaga ttgcacgttg acaggccgcc gcgagcggtg aaagcatggc gcccatgatc
     6721 acacatcgct tgtttatggg gaccacgctg ctcgcgtgcg cgctcgcgct cggggcgaac
     6781 tcgaactcgc gcgctgccag ccggtacgca ccgctgatgg cttcgtcggc cgggcaagac
     6841 acgctgcgca acctggcgct ttgggaggac cagcgcgtca ccggcaacgg gaagatctac
     6901 gactatctgc aaaacgggag ccccttggtg cggcggcgcg cggtggaagc cctcggtcgc
     6961 atgcaggagc cgagcgacgc cgccaggctc atcccgctgc tcaaggaacg cgactcggag
     7021 gtgttgcgcg agaccatctt cgcgctcggc cagatcggca accgcgaggc ggtagcgccg
     7081 ctcttggccg cgcgctcggg agcgctcccc gacgtggccg gtttgatcgc cgaggcgctg
     7141 ggcaagctcg gcggcgacga ggcgatcgcc gcgctcgacg cgatgcagcg cgacttcgcg
     7201 acctcggtgc gcgccgatgc cgccctggcg ctcgcgcgca cgccggatcc caacgcggcc
     7261 aacgcgctgc tcctggccgt gcacgacccc gatccgtccg tgatgtggcg cgccgcctac
     7321 gccttggaga agcagacgcc gatgccgcgc tcgtgcacgg cgatccagcc gctgctcgaa
     7381 aacgacgacc cgctggtgcg tgcctacgcg gtccgttcgc tcggaaagct ccagtgcaaa
     7441 caggccggca agccgctcgc ctcgctgctc aaggacgccg acctgcgcgt ggtcgtcaac
     7501 gcggcgcgcg cgctgggcga gatcaaggac aagggcgccg tgcacccgct cgcgcagatc
     7561 atcgccgacc acgagtcgca ccacgcgcgc gcggcggcgg cggaagccct cggcaaaatc
     7621 gacgacgaga acggcaagga cgcgctcgcg cagggactcc tcgacaccag cgcgatggta
     7681 cgaatcaact ccatccgcgc gctcgccgag gtcctgggcg agaacgccga gatgttctgc
     7741 gaccagatgc gccgcgacgg gagccggctg gtgcgtgcgg aagcgatacg aagctacggg
     7801 acggcgggcc tggccaagcg cgcttcgcag atcgacgaga tcgctcgcac cgacaaggat
     7861 ccgctgatgc gcgcggccgc catcgaggcg ctggcaaagc tcaaggacgc gaaggtagcc
     7921 ccgctgctcg tcccggcgct gcgcgatgcc gacttcacgg tcgccaccgc cgcggtggtc
     7981 gccatcggcg atcagcagta caaggccgcg gtccccgcgc tcatggacgc ctaccgcgcg
     8041 cggccggagc gcgagttcgt cgacgtgcag ctcgaagcgg ttcgggtgct gggtgatctc
     8101 ggcgcgctcg aatcggaggc gctgctggtc gaagccacct cgcacgacga cgcgcgcgtg
     8161 cgcgcggccg cggtcgcgtc tctcaagaaa ctggggctga cggctcccga gcttccgtcg
     8221 gagcgcacct tccacgagca ggccttcgac cccgcacgca agaagcagct cgccccgccc
     8281 gcggggacgc ggcacgccgt catcgcgacc cgccacggcg ccatcgagat cgagctattc
     8341 ggagacgacg cgattcaaac cgttgcgaac ttcatccacc tcgcgaagag cggtttctac
     8401 aagaagctca ccacccaccg cgtggtgccg aacttcgtcg tgcaaggcgg cgacccgcgc
     8461 ggcgacggct ccggcgacgc cggctacacg gtccccgccg aagtctcgcg ccaccacttc
     8521 ggcgaaggct tcctgggaat cgcggacgcg ggcaaggaca ccggcagctg ccagtggttc
     8581 atcacgctct cgccgcagcg acacctcgac gggcgctaca cgatcttcgg gcgggtgacc
     8641 aaggggatgg acgtggtgtg gaagatcgac caaggcgacg cgttcgacgt gaagatcctg
     8701 gactgacgcg accgacgcgg cgcgagccaa ggaccgactg gccgcatcat cccttccgca
     8761 aatcgtcgag tgcttgcgcg tgcgccttga acgcgatctc tttgggcgtg cggtccagcg
     8821 ggaagaagcg cgcgtcgagg gcgtcgtccc ccgcacgcaa gtcgcctccg attcgccggc
     8881 accggtacag caagagcacc gcgcgcacgc gcgggtcgtc catcgccatg taggcgccga
     8941 acaacccgtc cagttcgacg tcgaggttcg tctcttcctt ggtctcgcga atcgcgcact
     9001 ccgtgacgtg ctcgtcgtac tcgacgaacc ccgcgggcag cgtccacatg ccgacccgcg
     9061 gttcgaactt gcgctgcacc atgagcactt cgcccgcctc gaccagcacc acgcccgcgg
     9121 ccggcgcggg gttacggtag gcgacgaaac cgcagcggtc gcacacggcg cgaacgcggt
     9181 cgtcgacgcg gcgcggcgcc atgggaccgc cgcagcgtgc gcaaaagagc gcgtcctggc
     9241 tcatccttgg gacgcgcgca gcctctgccg cgtcagccac gccgcctcga tctcgctcaa
     9301 cggaatcccg tacaccttct cggtggcctg cgcgacacct tcgtacgcgg tcatcccgtt
     9361 ggctgccttg tagagctcca tgagcttctc gacgccgaac cggtcgacta aaaaccccac
     9421 gtaggaggct gcggccggca tcgatatcgg gggttcgatg gacatgaaga ggttgtactc
     9481 gaacatgctc cgcaagggcg gcacgagatt cgcgcgcacc agcttcttca tcgcttcttc
     9541 caccgggagt ccgtcgacgt cgtcgtgcag ccagaacacg gttccctcgg ccagcgcgcg
     9601 gggcgctcgc ccgatggggt cggtcatgaa gtgcacgatc tcgtggttgt cgttcgcgtt
     9661 gatggagtga aactcgacgt cgtcccagct cacgtactgg tagcccttga gactcagcat
     9721 gctgcgaatg gtggtggtgt cgggatagaa atagtattgg accacgacgt tgctctcgag
     9781 cccgagtttt ttcgcaatcg tatcccagat catctgctgc gccgcttgcg aaccgtcggg
     9841 catgggatgc cccggcaacc agtggaacac gaagttcttg gtggtcgcgg tttgccaatc
     9901 gatgggcgtg ggtacttcga gcgaaacgtt gtcgaaccag gcctggcccg tcatggccag
     9961 cacgcacgat accttcacga accgagtccc ctgcggcagc cggaacttga cgtcgtcctc
    10021 ggcccacagt cgagtgtcca ccaccggccg agtccgcttg tcggagaccc gcagctcctg
    10081 gaagcggtgg tgattctcgt cgtagaacgt cagcaggaaa ttgcagtgcg tcagttgatc
    10141 ggcgctcttc aagtcgacat tgtcggtctg aatccacccg cgcagtcgaa cgtgggtggc
    10201 atcgcgcact tccacttcct gcgaaagcag gaagaagcgc ttggtgttgg ggtcccccgc
    10261 gaagaaccag gagcgcttgc cgtcctgcgc gatctggtcg tcgacgccgt agcgaatggc
    10321 gaactcgttc tcgggcccgc ggaagttctc gatgctccag ccccgcggga tgccgtcgcg
    10381 gccgacgtcc tcgaacgaag cgttcaccag caggttggtg tcgtagtcgg gcttcccctt
    10441 gccgcacgac gccatgagcg ccgccgccgc caccagcagc gccgcgcccg cgtaccctgt
    10501 cctccgcaat ctcgttgtct gcatcatttc ttggccagct cctccgacca ctggtcgagt
    10561 cgatagatca acatgttctg ctccgcccgg acgatccgct tcttggcgtc cacatagagc
    10621 gtcgcttcgc ccaggcgctc gcgctccgcc ttgtacaccg tgacgcgggt cttcttgccc
    10681 agcaccgcga tggtttcttg ccccgcgacg tacagctcgc ccgcatccaa cacgcccgac
    10741 gccgggtcga gccactggat gcgctggcga ccctcgctcg ccgggtcgac ccagaacagg
    10801 agctggtacc acgggtggac cgtccccacc tcctggatcg ccgtgcccgc cggaaccacc
    10861 acccgccgcg agttcttggc gccgcgcaac tcgctcccga gcaccgcgac gttggcgacc
    10921 atgtctaccg tcagcgcatg cacgaactcg tcgtcggact gccgcacggt cttgtctgcg
    10981 cgatacgagc gcggaaaata cgactcctct tcgatgacga gttcgctccg ggtggtcatg
    11041 gcgacgcccg gattcgtgat caggttctcg gcatcgaagc ggatggtgtt gttgtcgaag
    11101 acgcggcgcg tcaccgactc ggtcccgatc tcctgatcgc cctgcagaat ggcgtacctc
    11161 gcgtgcgcgg tggagaccac gctcgcctcc ttggtagcgc gcatgggcgg ggccgcgtgc
    11221 agactggccg cggcgcacgt cgccaggccg atcgcgatcg cccaccgcac gcggcgcccc
    11281 gagcaccgag ggcttccctc gctccgat
//
LOCUS       DASPGK010000290         6123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1128044, whole genome shotgun sequence.
ACCESSION   DASPGK010000290 DASPGK010000000
VERSION     DASPGK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128044"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VEC56_07995"
     CDS             <1..762
                     /locus_tag="VEC56_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXV14133.1"
                     /translation="TDLNKPVLKRLNMEAHGTYHHSMLLGNIVDAIAAEIGADPLRAR
                     VMAYYHDIGKVFKPEYFAENQDPGFNKHEKITPQMSALILSSHVKDGVDLARQEKLPE
                     VIVDGIREHHGTTLMAYFYHKALEADAHATVNRDDFRYPGPRPRSKEAALIMCADAVE
                     AAVRSLRDPTPAHIRSMVVRLVDQRAHDGELDDSGITLSDLAVIKERLVAVMNTVYHK
                     RVAYPGQDGERAADERKSAAPEDWSATDESVAESG"
     gene            737..1186
                     /gene="ybeY"
                     /locus_tag="VEC56_08000"
     CDS             737..1186
                     /gene="ybeY"
                     /locus_tag="VEC56_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXV14134.1"
                     /translation="MKVSLSRASGRAPGWMDDAWRRRVARAAAGVGRRGATVNLVVVG
                     DREMRRLNRRYRGKDKPTDVLSFGYDAGPGDDVVGDVFVSYQTLARDARRLGVEARHL
                     AVRIVVHGLLHVVGYDHESDAEAARMERRERSVLKRVLPARVVRELF"
     gene            1202..2446
                     /locus_tag="VEC56_08005"
     CDS             1202..2446
                     /locus_tag="VEC56_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXV14135.1"
                     /translation="MGSSLIVAIYVVSVAFLAALSAALTSLGVLSLRRTEQDDADGDW
                     LHRRAVEDPVHTGVALGIARGAAMLAVVVSAVALVHAAGPVGAGVFAAASLLLPTFAA
                     RALALEGAGSLLRVVRPIVIPVVYLLRPVAILGSRLLGRASPALPRLFAFEVIPLAEK
                     MELIGTRGDDAAEGEEHRIISSIVDFGEKRARDVMVPRIDIVALDVKMDREELFAAIV
                     EAGHSRIPVYEGSIDRIIGTLHTQDLLIKVLSAEEISIRALARDAFFVPESKLISELL
                     AEFKVRRQHLAIVVDEYGGTAGIVTLEDVIEELVGDIRDEFDTEEELVRRLDRDTAVV
                     SARVRIEELNEMLGLDLVEDVADTLGGLLYHEIGRVPRVGDRWRHGVVEFEVQAVERQ
                     RVVRVVARGLSSIEPVAREDSV"
     gene            2537..3886
                     /gene="mgtE"
                     /locus_tag="VEC56_08010"
     CDS             2537..3886
                     /gene="mgtE"
                     /locus_tag="VEC56_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXV14136.1"
                     /translation="MEPTHIEDGGRLRALIEAGDAAGIEEFLGPLHSADIADILQQLD
                     VEEQIVCLKRLDTQRAAEVLAELDAKSGQALLTLLSDHEVGALLEELPSDDAADLIST
                     LTPEKTERVEALLPTADREEIQELLEFGAETAGGLMQVERVAVRDDSSIQGAIDVVRR
                     AAEEVESPQRVYVVNEAGKLLGELSILDLVIKPPTARVRDVMQLNVVAVPVDMDQKQV
                     AALFSKYDEFTMPVVDGQWRLVGRITMDDIIDVLEEEKSKDLARIAGTTEDELGETSI
                     FKVSRSRLPWLITGFIGEGVGAILMSRYEASLATFVVLAFFIPLIVGTAGNIGIQAAV
                     VVVRELALGQINIYRMGRRVLKELQVAFLNGLVLGGILFTLVYIWRDDPALAGLLWGS
                     LLLVIFVAAFMGSSIPLLLHRWRVDPAIATGPFVTVSNDVIGMAIYLTLATAYLKSH"
     gene            3947..4699
                     /gene="recO"
                     /locus_tag="VEC56_08015"
     CDS             3947..4699
                     /gene="recO"
                     /locus_tag="VEC56_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXV14137.1"
                     /translation="MLVKDSGIVISVARSGETSLGLVFLGRACGKVRLVAKGALSSKH
                     PGRGVLEPGNQVEVVYYHKDGFASYFLKEVSLVACPTAGRESLPHLAANLAALELLDQ
                     VCVPGASLDHEIVDTAAAYLQAPRVGDPLLLFLAFEIKLLDALGVAPDTLGCVHCGTT
                     PDGGLYSPRDGVSFCAEHGTRVPESVTLSEELLRTAARCAEASFEAIADGGVSRSARK
                     ELGRLVHWTYTYHVQGYHLPRSLSLIDSERRQ"
     gene            4749..5717
                     /gene="glyQ"
                     /locus_tag="VEC56_08020"
     CDS             4749..5717
                     /gene="glyQ"
                     /locus_tag="VEC56_08020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXV14138.1"
                     /translation="MTTRTKSRAARQSPAPTKQEIIMRLHEFWAKQGCVLVQPYNSEV
                     GAGTLNPATFLRVLGPEPWRAAYVEPSKRPRDGRYGENPNRVQQFLQYQVVLKPPPDN
                     VLQLYIESLRVIGIDPARHDIRLVEDDWEAPTLGASGLGWEVWLDGMEITQFTYFQQA
                     GSLELEPITAEITYGLLRLCLYLQRVDSVLDVTWAPGVSWGEMNLQAEQEFSAFHFEH
                     SNAEMHFKMFADFEKEAARMLAAGLVLPAYDYAVKLSHVFNLLDARGAISVTERTTYI
                     GRIRTLARKIALAYIAKREALGFPLLAGAAARAPKPARKPAGRKRA"
     gene            5714..>6123
                     /locus_tag="VEC56_08025"
     CDS             5714..>6123
                     /locus_tag="VEC56_08025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXV14139.1"
                     /translation="MSDFVLEIGFENVPASYLPPAIEQLSADAHAMLARCRLAHGEIY
                     TTATPRRLVLTVAGLADRQSQSEELVTGPPVARAFNPDGTPTPAAEGFARGHGVAVAA
                     LERVATPKGEYLGMRKRLPCARTSEVLPLELPALV"
BASE COUNT          994 a         2018 c         2109 g         1002 t
ORIGIN      
        1 accgacctca acaagccggt cttgaagcga ctcaacatgg aggcgcacgg gacctaccac
       61 cactccatgc tgctgggcaa catcgtggac gcgatcgcgg ccgagatcgg cgccgacccg
      121 ctgcgcgcgc gcgtgatggc ctactaccac gacatcggca aggttttcaa accggagtac
      181 ttcgccgaga atcaggaccc cggcttcaac aagcacgaga agatcacgcc gcagatgagc
      241 gccttgattc tctcctcgca cgtgaaggac ggcgtcgatc tcgcgcgcca ggagaagttg
      301 cccgaggtca ttgtcgacgg gatccgcgaa catcacggca ccactttgat ggcgtacttc
      361 tatcacaagg cgctggaggc cgacgcgcac gcgaccgtca accgcgacga cttccgctac
      421 ccgggcccgc gcccgcgcag caaggaggcg gccttgatca tgtgcgccga cgcggtggag
      481 gcggcggtgc gctcgctccg cgatcccacg cccgcgcaca ttcgctccat ggtggtgcgc
      541 ctggtcgacc agcgcgccca cgacggcgag ctcgacgaca gcggcatcac gctctccgac
      601 cttgcggtca tcaaggagag gctggtcgcg gtcatgaaca cggtctatca caagcgcgtg
      661 gcgtacccgg gccaggacgg ggagcgggcg gcggatgagc ggaagagcgc ggctcccgaa
      721 gattggagcg ccaccgatga aagtgtcgct gagtcgggct agcggccgcg caccgggctg
      781 gatggacgac gcctggcgcc gtcgcgtcgc gcgcgcggct gcgggtgtcg gtcgtcgcgg
      841 cgccaccgtc aatctcgtcg tggtgggtga ccgcgagatg cgccggctga accggcgcta
      901 ccgtggcaag gacaagccca ccgacgtgct gtcgttcgga tacgacgcgg gtccgggaga
      961 cgacgtggtg ggcgacgtgt tcgtgtcgta ccagacgctc gcgcgcgacg cgcgccgcct
     1021 gggcgtggaa gcgcgtcacc tcgcggtccg catcgtcgtg cacgggctcc tgcacgtcgt
     1081 cggctacgac cacgaatcgg acgcggaggc cgcgcgcatg gagcgccgcg agcgctccgt
     1141 gctcaagcgc gtgcttccag cgcgcgtcgt gcgcgagttg ttctgatggg gcaggaggtt
     1201 tatgggcagt agcctgattg ttgccattta tgtcgtgtcg gtcgcgttct tggcggcgct
     1261 ttccgccgcg ctcacctcgc tcggcgtgct ctcgctgcgc cggaccgagc aggacgacgc
     1321 cgacggcgat tggctgcatc ggcgcgcggt cgaggatccc gtgcacaccg gcgtggcgct
     1381 gggaatcgcg cgcggcgccg cgatgctggc cgtggtcgtt tcggccgtgg cactggtcca
     1441 cgcggccgga cccgtcggcg cgggggtgtt cgcggccgcc tcgctcctcc tgcccacctt
     1501 cgcggcgcgc gcgctcgcgc tggaaggggc cggatcgctc ttgcgcgtcg tgcgtccgat
     1561 cgtgatcccg gtggtgtacc tgttgcgtcc ggtcgccatc ctcggttcgc ggctgctggg
     1621 gcgagcttcg cccgcgctcc cgcgcctgtt cgcgttcgaa gtgatcccgc tggccgagaa
     1681 gatggagttg atcgggacgc gcggcgacga cgccgccgag ggcgaagaac accgcatcat
     1741 ttcgagcatc gtcgatttcg gcgagaagcg cgcgcgcgac gtgatggtgc cgcgcatcga
     1801 catcgtcgcg ctcgacgtca agatggaccg cgaagagttg ttcgcggcca tcgtggaggc
     1861 ggggcactcg cgcatccccg tctacgaagg ctccatcgat cgcatcatcg ggacgctgca
     1921 cacgcaggat ctcctgatca aggtcctgtc cgcggaggag atttcgatcc gcgcgctcgc
     1981 tcgagacgcg ttcttcgtcc ccgagagcaa gctcatcagc gagctgttgg ccgagttcaa
     2041 ggtgcgccgc cagcacctcg cgatcgtggt cgacgagtac ggcggcaccg cgggcatcgt
     2101 gacactcgag gacgtcatcg aagagctggt gggcgacatc cgcgacgagt tcgacaccga
     2161 agaagagctg gtgcgccgcc tggaccgcga caccgcggtc gtcagcgcgc gcgtgcgcat
     2221 cgaggaactc aacgagatgc tgggcctgga cttggtcgag gacgtggccg acaccctggg
     2281 cgggctcctg taccacgaga tcgggcgcgt gccgcgcgtg ggcgaccgct ggcgccacgg
     2341 ggtggtggag ttcgaggtgc aggcggtcga gcggcagcgc gtggtgcgcg ttgtggcgcg
     2401 cgggctatcg tcgatcgagc ccgtcgcgcg tgaagacagc gtgtaggcga aatcggcctg
     2461 gggacgcccg cccgcgaact cgtccttgcc agcgcgtcgc gtactcacct agcatgcgac
     2521 gaacgagttt tcctccatgg agccgaccca catcgaagac ggcgggcgcc tgcgggcgct
     2581 gatcgaagcc ggcgacgccg ccggcatcga ggagttcctg ggacccctcc actccgccga
     2641 catcgccgac atcctccagc agctcgacgt cgaagagcag atcgtctgcc tgaagcgcct
     2701 cgacacgcaa cgcgccgccg aggtgctggc cgagctggac gccaagtcgg ggcaggcgct
     2761 cctcacgctc ctgtccgacc acgaagtggg ggctctcctc gaggagcttc cctccgacga
     2821 cgcggccgac ctcatttcca ccctcacgcc cgagaagacc gagcgcgtcg aagcgctgct
     2881 ccccaccgca gaccgcgagg agatccagga gctgctcgag ttcggggccg agaccgcggg
     2941 cgggctgatg caggtcgagc gcgtcgcggt gcgcgacgat tccagcatcc aaggggccat
     3001 cgacgtcgtg cgacgcgcgg ccgaagaggt cgagagcccg cagcgcgtct acgtcgtcaa
     3061 cgaagccgga aagctgctgg gcgaattatc catcctcgac ctcgtcatca agcccccgac
     3121 ggcgcgcgtg cgggacgtga tgcaactgaa tgtggtcgcg gtcccggtcg acatggacca
     3181 gaagcaagtg gcggcactgt tctccaagta cgacgagttc acgatgccgg tcgtcgacgg
     3241 gcagtggcgg ctggtggggc gcatcaccat ggacgacatc atcgacgtgc tcgaggagga
     3301 gaagtcgaaa gatctggcgc gcatcgccgg caccaccgag gacgaactcg gcgagacgtc
     3361 gatcttcaag gtgtcgcgca gccgcctgcc gtggctcatc accggcttca tcggcgaggg
     3421 ggtgggtgcc atcctgatga gccgctacga ggcatcgctc gcgaccttcg tcgtgctggc
     3481 gttcttcatc ccgctgatcg tgggaacggc gggcaacatc gggatacagg ccgcggtcgt
     3541 ggtggtgcgc gagttggcgc tggggcagat caacatctat agaatgggac ggcgcgtgct
     3601 caaggagcta caggtcgcgt tcctcaacgg cctcgtgctg ggtggaatcc tgttcacact
     3661 ggtgtacatt tggcgcgacg accccgcgct ggcgggcttg ttgtggggat cgctcttgct
     3721 ggtgatcttc gtcgccgcgt tcatgggctc ctcgatcccg ctgctcctgc accggtggcg
     3781 cgtcgacccg gccatcgcca ccgggccgtt cgtgacggtc tccaacgacg tcattggaat
     3841 ggcgatatac ttgacgctcg cgacggcgta cttgaagagt cactaggatg aaggcatgcg
     3901 gcgctgggct cgcgtcgatg cttgcctcgc ggccggagat cgaaccatgc tcgtgaaaga
     3961 cagcggcatc gtcatttcag tcgcgcgcag cggcgagacc agcctggggc tggtgttcct
     4021 cgggcgcgcg tgcggaaagg tgcgcctggt ggccaagggg gcgttatcgt cgaagcaccc
     4081 ggggcggggc gtgctcgagc cgggtaacca ggtggaggtc gtttactatc ataaggacgg
     4141 cttcgcgagc tatttcctca aggaggtctc gctggtcgcg tgtccgaccg cggggcgcga
     4201 gtcgctcccg cacctggccg ccaacctggc cgcgctcgag ctgctggacc aagtgtgcgt
     4261 gccgggtgcc tccctcgacc acgagatcgt cgacacggcc gcggcgtact tgcaggcgcc
     4321 ccgcgtcggc gacccgctgc tcctctttct cgccttcgag atcaagctgc tcgacgcgct
     4381 cggggtggcg cccgatacct tggggtgcgt ccactgcggc accactcccg acggcggctt
     4441 gtacagcccg cgcgatggag tgagtttctg cgcggagcac ggcacccggg tacccgagtc
     4501 ggtcacgcta tcggaagagc tcttgcgcac ggcggcgcgg tgcgcggaag cgtccttcga
     4561 agcgatcgcg gacgggggcg tgtcgcgctc ggccagaaaa gagttggggc ggctcgtaca
     4621 ctggacctat acttaccacg tccaagggta ccacctgcca cgatcgttga gtctcatcga
     4681 cagtgagcgt cgacagtgag cgtcgcccac gcaacgcggc gaaataatcc cgaaaggact
     4741 ccgagtacgt gaccacgcgc accaaatcgc gcgccgcgag acaatcgccc gcgcccacca
     4801 aacaagagat catcatgcgc ctgcacgagt tttgggcgaa gcaggggtgc gtgctggtgc
     4861 aaccgtacaa ctcggaagtc ggcgccggca cgctgaaccc ggccacgttc ctgcgcgtgc
     4921 tgggtcccga gccctggcgc gcggcctacg tcgagccgtc gaagcgcccg cgcgacggcc
     4981 gctacggcga gaatcccaac cgcgtgcagc agttcctgca ataccaggtg gtgttgaagc
     5041 cgcccccgga caacgtgttg cagctctata tcgagagcct gcgcgtgatc ggcatcgacc
     5101 ccgcgcgcca cgacattcgt ttggtcgaag acgactggga ggccccaacc ctaggtgcgt
     5161 cgggcctggg ttgggaagtg tggctcgacg gcatggaaat cacgcagttc acctacttcc
     5221 agcaggcggg atcgctcgag ctcgagccga tcacggccga gatcacgtac ggactcttgc
     5281 gcctgtgttt gtacttgcag cgcgtcgact cggtgctcga cgtgacgtgg gcgcccgggg
     5341 tctcgtgggg cgagatgaac ctgcaggccg agcaggaatt ctccgcgttc cacttcgagc
     5401 attcgaacgc ggagatgcac ttcaagatgt tcgcggactt cgagaaggaa gcggcgcgca
     5461 tgctggccgc cgggctggtg ctgccggcct acgactacgc ggtcaagctc tcgcacgtgt
     5521 tcaatttact cgacgcgcgc ggcgcgatca gcgtgaccga acgcacgacc tacatcggtc
     5581 gcattcgcac gctcgcgcgc aagatcgcgc tcgcgtacat cgccaagcgc gaagcactcg
     5641 ggttcccgct gctggcgggg gcggccgcgc gcgcaccgaa gcccgcgcgc aagcccgccg
     5701 ggaggaaacg cgcgtgagcg acttcgtcct ggagatcggc ttcgaaaacg tgccggcgtc
     5761 ttatctgccg cccgcgatcg agcaattgtc ggccgacgcg cacgccatgc tggcgcgctg
     5821 ccgtctcgcc cacggcgaga tctacaccac ggccacgccg cgccgtctgg tactgacggt
     5881 cgccggcctg gccgaccgcc agtcgcagtc cgaggagcta gtgacggggc cgccggtcgc
     5941 gcgcgcgttc aaccccgacg gaacccccac gcccgcggcc gaagggttcg cgcgcggaca
     6001 cggtgttgcc gtcgccgccc tggagcgcgt cgccacaccc aagggcgaat acctcgggat
     6061 gcgcaagcga ctgccgtgcg cgcgcacttc cgaggtcttg ccgctcgaat tgcccgcgct
     6121 ggt
//
LOCUS       DASPGK010000291         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_660067, whole genome shotgun sequence.
ACCESSION   DASPGK010000291 DASPGK010000000
VERSION     DASPGK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660067"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VEC56_08030"
     CDS             <1..479
                     /locus_tag="VEC56_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HXV14140.1"
                     /translation="SNPFPMSIYENVVYSLRVDGVRDRATLDDVCEKSLRGAALWSEV
                     KDRLNESALGLSGGQQQRLCIARAIAGEPEVLLMDEPCSALDPIATGKIEDLMQELKG
                     AYSIVIVTHNMHQAARVSDYTAFMFLGRLIEYGPTEDIFTNPRLSETSDYVTGRFG"
     gene            556..1293
                     /locus_tag="VEC56_08035"
     CDS             556..1293
                     /locus_tag="VEC56_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV14141.1"
                     /translation="MLSSNMILEKRQTVLKTEKIVVIEDETDILEVIAYNLKREGYDV
                     VTSTSGEDGLEKIEKFKPNLVVLDLMLPEVDGIELCRKLKADPVTQSIPVIMVTAKGE
                     ESDVVLGLGVGADDYVTKPFSPRELTARVKAVLRRSKSKGEADRRERVTVEGVVIDPQ
                     RHEVKVDGEDVLLTATEFRLLHFLASHPGRVFTRDHLLTRVIGEEAIVIDRNIDVHVR
                     AIRKKLGPRRELIETIRGVGYRFKDRD"
     gene            1301..3097
                     /locus_tag="VEC56_08040"
     CDS             1301..3097
                     /locus_tag="VEC56_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV14142.1"
                     /translation="MLGSRFLWKLYAGYAVVILLTAVVVGVLVVRGFERDLTEQIQAS
                     LHSEALLLQDIARPYVDTPPDSAFQHRVWLLGSRADTRLTVIAADGRVLADSREDPSA
                     MDNQRTRPEIVGALTKGVGVAKLFSSSLGGTAMYVAVPVTKNDTVAGYVRVAKPLLAL
                     EQRRGAVRGMVLLGTVLAVLLALLIGLVLARNYTRPLVGMTTAARAVAAGDYDHRVRV
                     DRKDEIGELAEALNAMTAQLKSQIDTITADRNVTLAILASMVEGVVAVDSDDYVVHVN
                     SAAEAILGIDATSVRGRRIWEVTRVVEVSEALADAMKEDRVRVSEARISKPQKDQVIQ
                     LIGSPLKDAAGERVGAVLVMHDVSELRQLEGVRRDFVANISHELKTPLAAIRGLVETL
                     IDDQDMDPATHNRFIEKIHDQSQRLSSLVSDLLTLSRLESDQGGVQFEAVDLREAVAE
                     SYRAQVHAAETKRVEIAARVSDAPVVIEGDAEALRELVDNLLSNALKFTPEGGRVDVR
                     LGVEGPNAVLTVEDTGIGIAPEDQGRIFERFYRVDKARSRQLGGTGLGLSIVKHVALA
                     HGGNVALKSAPGRGSTFRIQIPLRRATGPGTS"
     gene            3204..3857
                     /gene="phoU"
                     /locus_tag="VEC56_08045"
     CDS             3204..3857
                     /gene="phoU"
                     /locus_tag="VEC56_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXV14143.1"
                     /translation="MAKHLQRDLDLLAKDLLTMGALVEDATNKAINALARRNKTLAQE
                     VAAGDHVINDRENQIEESALKILALHQPVAADLRFIITALKVNNDLERIGDHAVSIAE
                     RAETLADLESVPLPDEFHKLVEVVQHMVHDSLNALVERDAALARSVCMVDDEVDRIHR
                     FMYSAMQTVMRENAAYIEPAINTISATRHMERIADLATNIAEDVVFMVEGEILRHNY"
     gene            complement(3854..>6046)
                     /locus_tag="VEC56_08050"
     CDS             complement(3854..>6046)
                     /locus_tag="VEC56_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV14144.1"
                     /translation="GRLWMGTASGINCLALDRSNPPPASPRRTRVRVLGRDGVLASYR
                     NTSVYTCVRSLLPGPDGAVEGLWFAGIQNLYCLVDERWFVLRERAGFPVTSFHAVAVD
                     DSGHVWVGTRDRGLFRSVAPLTLAALEASATETVPVQPEEGHGAFGREITDALFEPVW
                     SRDLGAPTNQIEAVLWRDGSLWVGTPVGLFRFEGTPPRMADHFTREDGLQATNATAMA
                     FSPTGSLWVGTNGGLTEIDPVRRKVTRSVTRQDGLVGNEVWFYGSVAAGDDGTVYFGT
                     AQGLSLYAPHRDQPNPTPPLVRLRNVRYRENARGQNELAVEYAALSYSKESRVRYRTR
                     LVGYEREWSTETSEVKTRYTNLPAFFLPRRYEFQVAAHNGDGVWTATPLSHAFQVSPP
                     VWLRWWALVGQFVLVGGCLYGLHAHRTRSLERRAQLLEHVVDVRTQEVRAQALTLQEQ
                     NVELEAKNAEIVRTQQQLIVQEKLASLGALTAGIAHEIRNPLNFVNNFAELSGDLVQD
                     LRDDLSQQKSKLDESAARGIDDLLGDLEQNMQRINEHGKRANSIVQGMLLHSRGQKGE
                     FQKVKLNALLDEFATIAYVGMKAADPAMGVALEKDFDDAVGEIDAIPQDLSRVFVNIV
                     NNACYAATEKQRTSGGGYQPTVRLTTKSLGDRVEVRIRDNGNGIPEAIREKIFAPFFT
                     SKPTGAGTGLGLSISFDIVVQEHHGELRVETKEGEFAEFVMTLPRRNN"
BASE COUNT         1077 a         1982 c         1999 g          988 t
ORIGIN      
        1 agtcgaaccc attccccatg agcatctacg agaacgtggt gtattcgctg cgggtggacg
       61 gtgtgcgcga ccgagccacc ctcgacgacg tgtgcgagaa gagcctgcgc ggggcggctt
      121 tgtggagcga ggtcaaggac cgcctgaacg aaagcgcgct cgggctctcg ggcggacagc
      181 agcagcgctt gtgcatcgcg cgcgcgatcg cgggcgagcc cgaggtgctc ctgatggacg
      241 agccctgctc cgcgctcgac cccatcgcga ccggcaagat cgaagatctc atgcaggagc
      301 tcaagggcgc ctattcgatc gtcatcgtca cccacaacat gcaccaggcg gcgcgcgtca
      361 gcgattacac cgccttcatg ttcctggggc gcctgatcga gtacggaccc accgaggaca
      421 tcttcaccaa cccgcgcctg tcggagactt ccgactacgt gacggggcga ttcggataag
      481 cgaaggacaa cgccttgcca agggccggtc gggacgattt acgccgcgtt tacgcgatct
      541 ttttgaagga gcgctatgtt gtcgagcaat atgatcttgg agaagaggca gaccgtgctc
      601 aagactgaga agatcgtcgt catcgaggac gagaccgata ttctcgaagt catcgcgtac
      661 aacctcaagc gcgagggcta cgacgtggtc accagcacca gcggagaaga cggtctcgag
      721 aagatcgaga aattcaagcc gaacctggtg gtgctggact tgatgctgcc ggaagtcgac
      781 ggcatcgagt tgtgccgcaa gctcaaggcg gatcctgtca cgcagtcgat tccggtcatc
      841 atggtcacgg ccaagggcga ggagagcgac gtcgtccttg gcctcggcgt cggcgccgac
      901 gactacgtca ccaagccctt cagcccgcgc gagctcaccg ctcgcgtcaa ggcggtgctg
      961 cgccgctcga agtcgaaggg cgaagccgac cgccgcgagc gggtaacggt cgaaggcgtc
     1021 gtgatcgacc cgcagcgcca cgaggtcaag gtggacggcg aggacgtgct cctgaccgcc
     1081 accgagtttc gactgctgca cttcctggct tcgcacccgg gacgcgtgtt cacgcgcgac
     1141 cacctgctca cccgcgtcat cggcgaggaa gcgatcgtta tcgaccgcaa catcgatgtg
     1201 cacgtgcgcg cgattcgcaa gaagctcggc ccgcgccgcg agctgatcga gaccattcgg
     1261 ggcgtaggct accggttcaa agaccgagat tagaacgctg atgctcggct cgcgtttcct
     1321 ctggaagctg tacgccggct acgcggtcgt gatcctcttg accgcggtcg tggtcggcgt
     1381 tctcgtcgtg cgcggcttcg aacgcgacct caccgagcag atccaggcgt ctctccatag
     1441 cgaggcgctc ctcttgcagg acatcgcgcg cccctacgtc gacacacccc cggactccgc
     1501 gttccaacac cgcgtgtggc tgctcggctc gcgcgccgac acgcgcctga cggtgatcgc
     1561 ggccgacggc cgcgtgctgg ccgactcgcg cgaggacccc tccgcgatgg acaaccagcg
     1621 cacgcgcccc gagatcgtgg gcgcgctcac caagggcgtg ggggtggcga agctcttcag
     1681 cagttcgctc ggaggcaccg ccatgtacgt cgcggtgccg gtgacgaaga acgacacggt
     1741 ggcgggctac gtgcgcgtgg ccaagccgct cctggcactc gagcagcgcc ggggcgcggt
     1801 gaggggcatg gtgctgctcg gaaccgtgct ggcggtcctg ctcgcactct tgatcgggct
     1861 cgtgctcgcg cgtaactaca cgcgcccgct ggtcggcatg accacggccg cgcgcgcggt
     1921 cgcggccggc gactacgacc accgcgtgcg cgtggatcgc aaggacgaga tcggcgagct
     1981 ggcggaggcg ctcaacgcca tgacggcgca gctcaagtcc cagatcgaca ccatcaccgc
     2041 cgatcgcaac gtaacgctcg ccatcctcgc cagcatggtc gaaggggtgg tggcggtgga
     2101 cagcgacgac tacgtggtgc acgtgaacag cgccgccgaa gcgatcctcg gcatcgacgc
     2161 caccagcgtg cgggggcgtc gcatctggga agtcacgcgc gtggtggaag tgagcgaagc
     2221 gctggccgac gccatgaagg aagatcgcgt gcgggtatcg gaagcgcgca tctcgaagcc
     2281 gcagaaggac caggtgatcc aactcatcgg atcgccgctc aaggacgcgg ccggcgagcg
     2341 cgtcggcgcg gtactcgtca tgcacgacgt gagcgagctg cgccagctcg aaggcgtgcg
     2401 ccgcgacttc gtcgccaata tctcgcacga gctcaagacc ccgttggccg cgattcgcgg
     2461 gctggtcgag acgctcatcg acgaccagga catggacccc gcgacgcaca atcgtttcat
     2521 cgagaagatc cacgaccaat cgcagcgctt gagcagcctg gtctcggatc tcctcacgct
     2581 gtcgcgactc gagtcggacc agggcggggt ccagttcgag gccgtggacc tgcgcgaagc
     2641 ggtcgcggag tcgtaccggg cccaagtcca cgccgcggag accaagcgcg tcgagatcgc
     2701 ggcgcgcgtg tccgacgccc cggtcgtgat cgaaggcgac gcggaggcgc tgcgcgagct
     2761 ggtcgacaat ctactgagca acgcgctcaa gttcacgccc gagggcggga gagtcgacgt
     2821 gcgcctgggg gtggaggggc ccaacgcggt gctgaccgtc gaggacaccg gcatcggcat
     2881 cgcgccggag gaccagggcc gcatcttcga gcgtttttac cgcgtcgaca aggcgcgctc
     2941 gcgccagctg ggcgggaccg ggttggggct ttcgatcgtc aagcatgtgg ccctggcgca
     3001 cggcggcaac gtggccttga agagcgcccc cgggcgcggc agcaccttcc ggatccagat
     3061 cccgctgcgg cgcgcgaccg gcccgggaac gagctaggcg gaatggggtg agtggcatgc
     3121 ccgaatccga aagccaaaga ttgacgtggt ttggcactta ctctaaagat acgattaaga
     3181 acgcagaagc gattcaacgc ggcatggcca aacatctgca acgagacctc gatctactcg
     3241 ccaaggacct cctcaccatg ggggccctgg tcgaggacgc caccaacaag gccatcaatg
     3301 cgctcgcgcg tcgcaacaag acgctcgcac aggaggtcgc cgccggcgac cacgtcatca
     3361 acgaccgcga gaaccagatc gaagagagcg ccctcaagat cctcgcgctc caccagcccg
     3421 tcgccgccga cctgcgcttc atcatcaccg cgctcaaagt gaacaacgac ctcgagcgca
     3481 tcggcgacca cgcggtcagt atcgcggagc gcgccgagac cctcgcggat ctggagtcgg
     3541 tgccgctccc cgacgagttc cacaagctgg tggaagtcgt gcagcacatg gtgcacgaca
     3601 gcttgaacgc cctggtcgag cgcgacgccg ccctcgcgcg ctcggtgtgc atggtcgacg
     3661 acgaagtcga ccgtatccat cgcttcatgt actcggcgat gcagaccgtg atgcgcgaaa
     3721 acgccgccta catcgagccc gccatcaaca cgatcagcgc cacgcgccac atggagcgca
     3781 tcgccgacct cgccaccaac atcgccgaag acgtggtctt catggtcgaa ggcgagattc
     3841 tccgccacaa ttactaattg ttgcgccgcg gcagcgtcat cacgaactcc gcgaactcgc
     3901 cttccttggt ttccacacgt agctccccgt ggtgttcctg taccacgatg tcgaaactga
     3961 tggagagtcc gaggccggtg cccgcgccgg tgggcttgct ggtaaagaag ggcgcgaaga
     4021 tcttctcgcg gatcgcctcc ggtatgccgt tgccgttgtc ccggatgcga acctcgacgc
     4081 gatcgcccag actcttggtg gtcaggcgaa cggtgggctg gtagccaccg ccgctcgtcc
     4141 tttgcttttc ggtcgcggcg tagcacgcgt tgttgacgat gttgacgaag acgcggctca
     4201 agtcctgggg aatcgcgtcg atctcgccca cggcgtcgtc gaagtccttt tcgagcgcca
     4261 cgcccatggc cgggtctgcg gccttcatcc cgacgtaggc gatggtggcg aattcgtcca
     4321 gcagggcgtt gagcttgacc ttttggaact cgcccttctg gccgcgcgag tgcagcagca
     4381 tcccctgcac gatgctgttc gcgcgtttgc cgtgctcgtt gatgcgctgc atgttctgct
     4441 cgaggtcgcc caggaggtcg tcgatgccgc gcgcggcact ctcgtcgagc ttgcttttct
     4501 gctgggacag atcgtcgcgc aggtcctgca cgaggtcgcc ggaaagctcg gcgaagttgt
     4561 tgacgaaatt gagcgggttt cttatctcgt gcgcgatgcc ggcggtcaaa gcgcccagcg
     4621 aggccagctt ctcctgcacg atcagctgct gctgggtgcg cacgatctcg gcgttcttgg
     4681 cctcgagttc gacgttctgt tcctgcagcg tcagcgcctg cgcgcgaact tcctgcgtgc
     4741 gaacgtcgac cacgtgctcg agcagctgcg cccgacgctc caggctgcgg gtacggtgcg
     4801 cgtggaggcc gtagaggcat ccgcccacca gcacgaactg acccaccaac gcccaccagc
     4861 gcaaccacac gggcggagag acctggaacg cgtggctcag gggcgtcgcc gtccacacgc
     4921 cgtcgccgtt gtgcgcggcg acctggaact cataccggcg cggcaggaag aaagccggca
     4981 ggttggtgta gcgcgtcttt acttcggacg tttcggtgga ccactcgcgc tcgtagccga
     5041 ccaatcgcgt gcggtagcgc acccgggact ctttggagta gctcagggcg gcgtactcga
     5101 cggccagctc gttttgtccg cgtgcgttct cgcggtagcg cacgttgcgt aagcgcacca
     5161 gcggcggggt cgggttgggc tggtcgcgat gcggcgcgta taacgaaagt ccctgcgccg
     5221 ttccgaagta cacggtacca tcgtcgcccg cggcgaccga cccgtagaac catacttcgt
     5281 tgcccaccag tccgtcctgt cgggtcacgg agcgagtgac cttgcgacgc accggatcga
     5341 tttcagtgag ccctccgttg gtgccgaccc agagcgagcc cgtgggggaa aaggccatgg
     5401 cggtcgcgtt ggtcgcttgc aggccgtcct cgcgcgtgaa gtggtcggcc atgcggggcg
     5461 gggttccctc gaagcgaaac agtccgaccg gcgtcccgac ccacagcgag ccatcgcgcc
     5521 acaggaccgc ttcgatctgg ttggtgggag cgccgaggtc gcgcgaccac acgggctcga
     5581 agagcgcgtc ggtgatttcg cgtccgaacg cgccgtggcc ctcttcgggt tgcacgggga
     5641 cggtttcagt cgcggacgcc tcgagcgccg ccagcgtgag cggcgcaacg cttcgaaaca
     5701 ggccgcggtc gcgcgtaccg acccacacgt gcccgctgtc gtcgaccgcg accgcgtgga
     5761 acgacgtcac cgggaacccg gcgcgttcgc gcaggacgaa ccatcgctcg tcgaccaagc
     5821 aatagagatt ctggatgccg gcgaaccaaa gtccctcgac cgcgccgtcg ggtcccggca
     5881 ggagcgaccg aacgcacgtg tacacggacg tgttgcggta gctcgcgagc acgccgtcgc
     5941 ggcccagcac gcggaccctg gttcgacgcg gcgaggccgg cggcgggttc gaccggtcca
     6001 gcgcaaggca gttgatgccc gacgcggtgc ccatccacaa ccggcc
//
LOCUS       DASPGK010000292         8306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_191818, whole genome shotgun sequence.
ACCESSION   DASPGK010000292 DASPGK010000000
VERSION     DASPGK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191818"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VEC56_08055"
     CDS             <1..2047
                     /locus_tag="VEC56_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV14145.1"
                     /translation="LGEFRGEFRGLVGTFEEYPRAAGDGHPGCFGATEIINGEELWKR
                     MDENWATRPDSRAFLKARLVDLLVGDWDRHRGQWRWAKLPAQELWQPIPEDRDQAFVR
                     FEGLVVNAGRMQLPQFVSFGDDYPGIDGLVWNGRDGDRRILVDMDRDQWMEVARDVQS
                     RIGDDVVAEAVERLPAPYRELEGAATEAALRSRRDNLLEIADVFYRFIARDVDVRLTA
                     QDELVRVIRGESGDVSVSVSPRNADSPAFYRRTFLADETDEVRIYLGGGADSVVSQGG
                     DGKITVRVIGGDGTDAIDDAAGVGLRVSDADTGTRLTPGHGTRLDSRPYTPPAREKAP
                     WIPPRDWGRRNIWYPFIGGNSDVGVLFLLGWQSEGYGFRKDPYSDKHVVRVGYATEAG
                     GFGGDYRGEYRRENSRSYAGLYLRGSGLDFLHFYGFGNESPSTEDEDFYKVKQSLYKI
                     EPSYTCPLAGPLMATVRANVAYAKTKLEPNQFITDVHPYGAEDFLQYGAGLGLSIDTR
                     DSDVAPMRGAHVAVDGTVFPAAADVASAFGEVHGEAAYYQPIGILSTPTLALRAGGKK
                     VWRDYPYYEAAYIGGSRTVRGFPQQRFAGDASVFGNAELRIPLTRIYIFVPGSLGVFA
                     LADIGRVYLEGESSDTWHTSAGGGIWCSFLNTANTASLAVAASEEGTRVYVLAGLAF"
     gene            2095..3747
                     /locus_tag="VEC56_08060"
     CDS             2095..3747
                     /locus_tag="VEC56_08060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV14146.1"
                     /translation="MAAQFQIRTESQPSSQSDPSNFSDLDSQTQRGSYHGLAAAASVY
                     AGAYFFAYVADWLTRVAADGYFHFMPAFDTVVATIAIGYASFVAYWCRRQDCEACMFT
                     RMATVFLIVGALGIAVLHWGWERENLALRFSVETVDWVGVWIVAFPAIVTLPPKQVLG
                     ASLGAAATVPAVMSASALVNGVPEEAGNLPWVTVLRYSMPVAICVGVAYASAYRVFRL
                     ARDVASARRLGSYQLKEKIGSGGMGEVWRARHMMLARPAAVKLIRPESLGKNGDSNSV
                     STMMRRFEREAQATAMLTSQHSIQLYDFGIADDGVFYYVMELLEGRDLKSLVHESGPV
                     PAERAVHFLRAACDSLADAHHRGLIHRDVKPANIFTCRRGRDFDFVKVLDFGLVKTVT
                     EPGETLTQLTGTNATSGTPGFMAPEMVTGEAPVDGRADIYALGCVAYWLLTGQLVFDG
                     KSPMSILVQHVKEEPPTLRSRTEIEVPPRLEEIVRACLAKDPDDRPTTAQELGDTLAE
                     LAATLPPWTQARAEKWWRTNLPNLYAAPLAGKLESSPSVVNG"
     gene            3740..6040
                     /locus_tag="VEC56_08065"
     CDS             3740..6040
                     /locus_tag="VEC56_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HXV14147.1"
                     /translation="MASSGRESGSASLVVIASVCAFAIIATQVAGKAARDALFLTYFD
                     ISALPLVLIASALVSIGAVLGTARSMTVLGPARAIPPWFFASAVLLAAEWLLAMRYPR
                     PAAVVIYLHIAVIGSILISGFWSIINESFDPRSAKRTIGRIVGGATIGGLFGGFLADQ
                     VGSRAGVLAVLPIVAGLHLVCALLLMRFRHGDGAADAPPLRSWFTRARRGHAVESGFR
                     ALKRVGYLRNLAVIVLLGNAAATLIDFMFKARAAETFTTSAELVRFFAVFYTAVSVVT
                     LIVQAGMTRRLLERIGIANLVAVRPAVMVVGGLATLPVLGLVGLGILRGVEGVLQSSL
                     FRSGYELLFLPVVPKDKRATKTIVDVGADRMGDVLGGAITRAVIFLPMAVADHILVLL
                     AVVISIAGFVVARALRRGYVRALEASLIDRANVLDIKEDEATGTSTTLMESFTGLDLS
                     MSGAGIRLDELRAAVRSPSPETPAPDAEVDPSAPQLAPAPIPAADPEIASLVALRSGD
                     ARRVHAELRHSRGLTPGVAAQVIALLAWDEVTGWASRALAKAAPSITGQLVDRLLDPN
                     EDFAIRRRIPRILGTCATPRSYDGLLAALADKRFEVRFQCGRALARINEMAPSLVVDK
                     NAVYAAVLQETRVEKALWQSQRLIDDPAVNDSLPIDETLRARASRSMEHVFTLLSLVL
                     PRAPLQIAFKGLLTSDAVLRGTSLEYLESVLPREIWTGLSPLLDESGSRAASARPREE
                     VLEELLRSNQSIELNLEELRNRIRSE"
     gene            complement(6041..>8306)
                     /locus_tag="VEC56_08070"
     CDS             complement(6041..>8306)
                     /locus_tag="VEC56_08070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV14148.1"
                     /translation="TMPFPALSERPGTIIGPYKLLEQIGEGGMGVVYMAEQTEPVARR
                     VALKIIKPGLDTRQVIARFEAERQALAMMDHPNIAKVLDAGATDSGRPYFVMELVKGV
                     PITQFCDVGHLTATARLELFLAVCHAVQHAHQKGVIHRDLKPSNILVSRVDQHAAPRV
                     IDFGIAKAAEAVAGAETMHTAFGSVIGTLEYMSPEQAAGGEAKVDTRSDVYSLGVVLY
                     ELATGALPFESTALRGAGPVEAQRLIRDTDPPTPARRFTGTSSRDAIARTRSTDARSL
                     ERRLTGDLGWVIMRALEKDPARRYQSANELAADLERLRRNEPVGAGPPSRRYRAARFV
                     RRHRTAVIAASVVFVALTAGITLATVGLVRAKRAQAVAESEALRATKIRDFLTNMLAQ
                     SRPDKRGPDVTVMEVVDSTAAQMESDTTFHDDPLVRADILHALGETYRTLDQYERALP
                     LFQAALALKRSAAGDNDRTILITLNKVSQTQAESGNLEDAIVTQEEVVALSERAIGKE
                     HEEYSARLSNLGNMYADVGELAKAEGILREAIAIDRRVLGNDHEDMPISLNNLATILV
                     DDGKCEDAIPLHEESLAMRHRAYGEPSAEMAVALGNYARALDCAGRFVEAEIAADSAL
                     TMCATVFGPRHHRTATCRVRLAEVLLHTNRAAAAEPLLREAIDVFESIDERFWRLGDA
                     RARLGEVLLAQGRGAEGVRELVAGWDILTETVKPDTPRSREIAAIIVSYYDGAQERSL
                     ADPWRVRAAGGSPE"
BASE COUNT         1235 a         2868 c         2806 g         1397 t
ORIGIN      
        1 cttgggagaa ttccgcggcg agtttcgcgg gctcgtggga acgttcgaag agtacccgcg
       61 cgccgcgggc gacggacacc ccgggtgctt cggcgcgacc gagatcatca acggcgagga
      121 gctgtggaaa cgcatggacg agaactgggc gacgcgtccc gactcgcgcg cgtttctcaa
      181 agcacgtctg gtcgatctcc tcgtcgggga ctgggaccgc caccgcggac agtggcgctg
      241 ggcgaagctg cccgcgcaag agctgtggca accgatcccc gaggaccgcg accaggcctt
      301 cgtgcgcttc gaagggctgg tcgtcaacgc gggccgcatg cagctcccgc agttcgtgag
      361 cttcggcgac gattatcccg gcatcgacgg cttggtctgg aacgggcgcg acggcgaccg
      421 ccgcatcctg gtcgacatgg atcgcgacca gtggatggaa gtcgcgcgcg acgtgcaatc
      481 gcgcatcggc gacgacgtgg tcgcggaggc ggtcgaacgg ctccccgccc cgtatcgaga
      541 gctcgaaggc gccgccaccg aggccgcgct ccgtagtcgg cgcgacaacc tgctcgagat
      601 cgcggatgtc ttctaccggt tcatcgctcg cgacgtcgac gtgcgcctga ccgctcagga
      661 cgaactcgtg cgcgtgatcc gcggcgagag cggcgacgtg agcgtctcgg tatcgccacg
      721 caacgccgat tcgcccgcgt tctaccggcg cacgttcctc gccgacgaga ccgacgaggt
      781 tcgcatctac ctcggcggcg gcgccgacag cgtggtgtcg cagggcggcg acggcaagat
      841 caccgtgcgc gtcatcggcg gagacggcac cgacgcgatc gacgacgccg ccggcgtggg
      901 cctgcgcgtc tccgacgcgg acacgggcac gaggcttacg ccaggccacg gcacccgcct
      961 cgactcccgt ccgtataccc ctcccgcgcg cgagaaggcg ccctggattc cgccccgcga
     1021 ctggggccgt cgcaacatct ggtatccgtt catcggcggc aacagcgacg tcggcgtgct
     1081 gttcctgttg ggatggcagt cggagggcta tggattccgc aaggacccct actccgataa
     1141 gcacgtcgtg cgcgtcggct acgcgaccga ggcggggggc ttcggcggcg actaccgcgg
     1201 cgagtaccgg cgcgagaact cgcgctcgta cgcgggcctc tacctgcgcg ggtcggggct
     1261 cgacttcttg cacttctatg gcttcggaaa cgagtcgccg tcgaccgagg acgaggactt
     1321 ctacaaggtg aagcagtcct tgtacaagat cgagccgtcg tacacctgcc cgctcgccgg
     1381 tcctctgatg gcgaccgtgc gcgcgaacgt ggcgtacgcc aagaccaagc tcgagccgaa
     1441 tcagttcatc accgacgtgc acccgtacgg cgccgaggac ttcctgcagt acggcgcggg
     1501 cctgggcctc tcgatcgaca cgcgcgactc cgatgtcgcg ccgatgcgcg gcgcgcacgt
     1561 cgccgtcgac ggcaccgtgt tccccgcagc ggccgacgtc gcgagcgcct tcggcgaggt
     1621 ccacggcgaa gcggcgtact accagcccat cgggatcctc tccacgccca cgctcgcgct
     1681 gcgcgcgggc gggaaaaaag tgtggcgcga ctacccgtac tacgaggcgg cgtatatcgg
     1741 gggcagccgc accgtccgcg gctttcccca gcagcgcttc gctggcgacg cgtcggtgtt
     1801 tggcaacgcc gagcttcgaa ttcccctcac gcgcatctac atcttcgtcc ccgggagcct
     1861 gggcgtcttc gccctcgcgg acatcgggcg cgtctacctc gaaggcgaat cgtcggacac
     1921 atggcacacg tcggcgggag gcggcatctg gtgctccttc ctcaacaccg ccaacaccgc
     1981 gagcctggcg gtggccgcga gtgaggaagg gacgcgcgta tacgtcctcg cgggtctggc
     2041 gttctagccg ggcccgtgct aaccttgcgg cgttccgttc gaaggagagc cgccatggcc
     2101 gcacaatttc aaatacggac ggagagccag cccagctcgc agagcgatcc gtccaacttt
     2161 tccgacctcg attcccaaac gcaacggggc tcgtaccacg gcctcgcggc ggccgcgagc
     2221 gtctacgccg gcgcgtactt ctttgcctac gtcgccgact ggctgacccg ggttgcggcc
     2281 gacgggtact tccacttcat gcccgcgttc gacaccgtcg tcgccactat tgcgatcggc
     2341 tacgccagtt tcgtcgccta ctggtgccgc cgccaagact gcgaggcgtg catgttcacg
     2401 cgcatggcga ccgtgttcct catcgtgggc gcgctcggga tcgccgtcct ccactggggg
     2461 tgggagcgag agaacctggc ccttcgcttc tcggtcgaga ccgtcgattg ggtcggcgtc
     2521 tggatcgtcg cctttccggc catcgtgacg ctgccgccca aacaggtgct gggggcctcg
     2581 ctcggcgcgg ccgccaccgt gcccgcggtc atgtcggcgt cggccctggt caacggcgtt
     2641 cccgaggaag ccggcaacct tccgtgggtc acggtgctgc gctattcgat gccggtcgcg
     2701 atctgcgtcg gcgtcgcgta cgcgagcgcg taccgtgtgt tccggctcgc gcgcgacgtc
     2761 gcgtcggccc gccgcctcgg cagctaccag ctgaaggaaa agatcggctc cggcggcatg
     2821 ggcgaagtat ggcgcgcgcg gcacatgatg ctcgcgcggc cggccgcggt gaaactgatt
     2881 cgccccgagt cgctgggcaa gaacggcgac tcgaacagcg tcagcacgat gatgcgccgc
     2941 ttcgagcgcg aggcgcaggc caccgcgatg ctcacgtcgc aacattcgat tcagctctac
     3001 gacttcggca tcgccgacga cggcgtgttc tattacgtca tggagctcct ggaaggacgc
     3061 gacctgaagt cgctggtgca cgaatcgggg cccgtgccgg ccgaacgcgc ggtgcacttc
     3121 ctgcgcgccg cgtgcgattc cctggccgac gcgcaccacc gcgggctgat ccatcgcgac
     3181 gtcaaaccgg cgaacatctt cacctgccgc cgcggtcgcg atttcgactt cgtcaaggtg
     3241 ctcgatttcg ggttggtgaa gaccgtcacc gagccgggcg agaccctcac gcagctcacc
     3301 ggcaccaacg caaccagcgg cacgccggga ttcatggcgc ccgagatggt gacgggcgaa
     3361 gcgccggtgg acggacgcgc ggacatctac gccctcggtt gcgtcgcgta ctggcttctc
     3421 accggccagc tggtgttcga cggcaagagc ccgatgtcga tcttggtgca gcacgtcaag
     3481 gaagagccgc cgacgctacg ctcgcgcacc gagatcgagg tgccgccccg gctggaggaa
     3541 atcgtgcgcg cgtgtctcgc gaaggacccc gacgaccgcc ccaccaccgc gcaggagctg
     3601 ggcgacacgc tcgcagagct ggccgccacg ctgcccccgt ggacgcaggc gcgcgccgag
     3661 aagtggtggc gcacgaacct gcccaacctc tacgcggcgc ctctcgccgg aaagctggag
     3721 tcgtcgccct cggtggtgaa tggctagctc gggccgggaa tccggttcgg cctcgctggt
     3781 cgtaattgcc tcggtgtgcg cgttcgcgat catcgccacc caggtcgcgg gcaaggcggc
     3841 gcgcgacgcc ctcttcctca catacttcga catctccgcg ctgccgctgg tgctgatcgc
     3901 ctcggcgctg gtgtcgatcg gcgcggtgct cggcaccgcg cgctcgatga cggtgctggg
     3961 tccggcgcgc gccatcccgc cctggttctt cgccagcgcg gtgctgctgg cggcggagtg
     4021 gctgctggcg atgcgttatc cgaggcccgc cgccgtcgtc atctatctcc acatcgcggt
     4081 gatcgggtcg atcctgatct cgggattctg gtcgatcatc aacgagtcct tcgacccgcg
     4141 cagcgccaag cggaccatcg gccgcatcgt tgggggcgcc accatcggag gactctttgg
     4201 cggcttcctg gccgaccaag tcgggtcgcg cgccggcgtc ctggcggtgc tccccatcgt
     4261 cgcgggcttg cacctggtgt gcgcgctgct cctgatgcgc ttccgccacg gcgacggtgc
     4321 agcggacgcg ccgccgctgc gctcgtggtt tacgcgcgcg cgccgcgggc acgccgtgga
     4381 atcgggcttc cgcgcgctca aacgcgtggg ctatttgcgc aatctggccg tgatcgtcct
     4441 gttgggcaac gcggccgcga ccctcatcga cttcatgttc aaggcgcgcg cggccgagac
     4501 tttcaccacc agcgccgagc tggttcgttt cttcgcggtg ttctacaccg cggtgtcggt
     4561 agtgacgctg atcgtgcaag ccggcatgac gcgccggctg ctggaacgaa tcggcatcgc
     4621 caacctggtc gccgtccgcc cggccgtgat ggtggtgggc gggctcgcca ccctcccggt
     4681 gctggggctc gtgggtttgg ggattctgcg cggcgtcgaa ggcgtgctcc agagctcgct
     4741 gtttcgatcg ggctacgagc tgcttttcct gccggtcgtt cccaaggaca agcgcgccac
     4801 caagaccatc gtcgacgtgg gcgcggatcg catgggcgac gtcctcggcg gggccatcac
     4861 gcgggccgtg atcttcctgc ccatggcggt ggcggatcac atcctcgtgc tgctggcggt
     4921 cgtgatctcg atcgcgggat tcgtggtcgc gcgcgcgctg cgccgcggct acgtccgcgc
     4981 tctggaggcg agcctcatcg accgcgccaa cgtgctcgac atcaaggaag acgaggccac
     5041 gggaacgtcg accacgttga tggagtcgtt cacgggtctc gacctgtcga tgtcgggcgc
     5101 cggtatccga ttggacgagc tgcgcgccgc cgtgcgttcg ccgtccccgg aaacgcccgc
     5161 accggacgcc gaagtggacc ccagcgcgcc gcagctggca ccggcgccga tccccgccgc
     5221 cgaccccgag atcgcgtcgc tcgtggccct gcgctccggc gatgcccgtc gcgtgcacgc
     5281 cgagcttcgg cattcgcgcg ggctaacgcc gggggtggcg gcgcaagtga tcgcgttgct
     5341 ggcctgggac gaggtcaccg ggtgggcgtc gcgcgcgctg gccaaggcgg cgccgtcgat
     5401 caccggccag ctcgtcgacc gcttgctcga tcccaacgag gacttcgcga ttcggcgccg
     5461 gattccgcgc atcctcggaa cctgcgccac gccgcggtcg tacgacggcc tgctggccgc
     5521 gctcgccgac aagcgtttcg aggtgagatt ccagtgcggg cgagcgctgg cgcgcatcaa
     5581 cgagatggcg ccctcgctcg tggtcgacaa gaacgcggtc tacgcggccg tgctccaaga
     5641 aacgcgggtc gagaaggcgc tctggcagag ccaacgcctc atcgacgacc cggccgtcaa
     5701 cgactccctg cccatcgacg agacgctgcg cgcgcgcgcc agtcgcagca tggagcacgt
     5761 attcacgctg ctctcgctgg tgttgccgcg cgcgccgctg cagatcgcgt tcaagggctt
     5821 gctgacgagc gacgcggtgt tgcgcggcac cagcctcgag tatctggaga gcgtcttgcc
     5881 gagggagatt tggacgggct tgagtccgct cctcgacgaa tcgggcagcc gggccgccag
     5941 cgcgcgtccg cgcgaggaag tgttggaaga actcctgcgc tccaaccagt cgattgagtt
     6001 gaacctggaa gagcttcgca atcggatccg gagcgagtga ctactcgggc gaaccacccg
     6061 ccgcgcgcac ccgccaggga tcggccaacg agcgctcctg ggctccgtcg tagtacgaca
     6121 caataatcgc cgcgatctcc cggctacgcg gcgtgtcggg tttgacggtt tcggtcaaga
     6181 tatcccaccc ggctacgagt tcgcgtactc cctccgcgcc gcgcccttgc gccagtagta
     6241 cctcccccaa acgcgcgcgc gcgtcgccca ggcgccagaa tcgctcgtcg atactctcga
     6301 acacgtcgat cgcttcgcgc aggagcggct cggcggcggc cgcgcgattc gtgtgcagaa
     6361 gcacttcggc cagccggacg cggcaggtcg cggtgcgatg gtgccgaggc ccgaacaccg
     6421 tggcgcacat ggtgagcgcg ctgtcggccg cgatctcggc ctcgacgaac cggccggcac
     6481 agtcgagcgc ccgcgcgtaa ttgcccaggg cgacggccat ttccgcgctc ggctctccgt
     6541 acgcccggtg ccgcatcgcc agtgactcct cgtggagcgg tatggcgtcc tcgcacttac
     6601 catcgtctac caggatggtg gcgaggttgt tgagcgagat gggcatgtct tcgtgatcgt
     6661 tgcccagcac gcggcgatcg atcgcgatcg cctcgcgcag aatcccctcc gccttcgcga
     6721 gttcgccgac gtccgcgtac atgttaccca ggttggaaag tcgggcgctg tactcctcgt
     6781 gctctttccc gatggctcgc tccgacagcg cgaccacttc ctcctgggtg acgatggcgt
     6841 cctccaggtt gcccgactcg gcctgcgtct gcgagacctt gttgagagtg atgaggatgg
     6901 tgcggtcgtt gtcccccgcc gcggaacgct tgagcgccaa ggcggcttgg aacagcggga
     6961 gggcgcgttc gtactggtcg agggtgcgat aggtttcgcc gagtgcgtgg agaatgtccg
     7021 cgcgcaccaa gggatcgtcg tggaaggtgg tatcactctc catttgcgcc gccgtggagt
     7081 caacgacctc catcacggtg acgtccggcc cccgcttgtc ggggcgcgac tgcgcgagca
     7141 tgttggtgag aaagtcgcga atcttggtgg cgcgcaaagc ctcgctctcg gcaacggcct
     7201 gcgcgcgctt cgcgcgtacg agccccaccg tcgccagcgt gatcccggcg gtcagcgcca
     7261 cgaagacgac ggacgccgcg atcaccgccg ttcgatgacg ccgtacgaaa cgcgccgccc
     7321 gataccgccg gctcggaggt cccgccccca cgggctcgtt tcggcgcagc cgttccagat
     7381 ccgcggcgag ctcgttggcc gattggtagc gacgcgcggg gtccttctcc agcgcacgca
     7441 tgatgaccca gcccaggtcg cccgtgagcc gccgctcgag cgagcgcgcg tccgtgctcc
     7501 gcgtgcgcgc gatggcgtcg cgcgacgacg tgccggtgaa acggcgcgcg ggcgtgggcg
     7561 ggtcggtatc gcgaatcaat cgctgcgcct ccaccggtcc cgctccgcgc aacgcggtgg
     7621 actcgaacgg cagcgccccc gtcgccaact cgtacagcac gacgcccagc gagtagacgt
     7681 cggagcgcgt gtccactttg gcctccccgc ctgccgcttg ctccgggctc atgtactcca
     7741 gcgttccgat aaccgacccg aacgcggtgt gcatggtctc ggcacccgcg accgcctcgg
     7801 ccgccttggc gatcccgaag tcgatgacgc ggggcgcggc gtgctggtcg acgcgggaga
     7861 cgaggatgtt ggacggcttg aggtcgcggt gaatgacccc tttctgatgc gcatgctgga
     7921 cggcgtggca gacggccaga aacagctcca gccgggccgt ggccgtcaga tgcccgacgt
     7981 cgcagaactg ggtaatcggc acccccttga ccaattccat cacgaagtac ggccggccgc
     8041 tgtcggtggc gccggcatcg agcaccttcg cgatgttggg atgatccatc atcgccagcg
     8101 cctgccgttc ggcttcgaag cgggcgatca cttgccgcgt gtccaggccg ggcttgatga
     8161 tcttcagcgc gacgcggcgt gcgaccggtt cggtctgctc ggccatgtag accacgccca
     8221 tgcccccctc gccgatctgc tccagcagct tgtacgggcc gatgattgtg ccagggcgct
     8281 cggagagagc aggaaacggc atcgtc
//
LOCUS       DASPGK010000293         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_895265, whole genome shotgun sequence.
ACCESSION   DASPGK010000293 DASPGK010000000
VERSION     DASPGK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895265"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VEC56_08075"
     CDS             complement(<1..1623)
                     /locus_tag="VEC56_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV14149.1"
                     /translation="MTKKDKQVQARRKPPTPPARTDLRDSLDAAQPFRWGNRADWLQL
                     GTVVGVALALRVAFYFLNRIYNPTFYFPVMDSLYHHEWALDLVAGGTPGTDAFFRGPM
                     YPYFLAALYKMSGSSIAFAVFIQHLIGALTAGVVYLTARELFSRSVSLVAGLAVALYW
                     VLVYMEGDLLLETLFIFFNSLTLLFLTMGIRRRRLWLLAAGGFALGIATITRPSVMVF
                     FPAVPLALWLAWKRRPEGRGWVAQSIVVAVACAIPILPVMIRNYAVAKAVVPVGASGG
                     VNFWIGNNPVSDGSTAIVPGTRADWWGGYEDAIAMAEKDVGKKLKLAEVSDYYFKKGF
                     DYIRGNPSEATAHMAKKFQMFWGAGERANDKYIYFFWHLAKMKYVPLPGFWLVAPLAI
                     LGGVLLWRRRSDLSMLYLFVGLYSVGVIAFFVNARFRLPIVPILCLFAAYGGVYVWRA
                     FREKQFGLVKALLVVAAAAALVNSDYLYQKQMRAYSDAFSNYTLGNAYLKMGSKGTAL
                     SHYTRADEINGQYPTPSYRLIQREVDYNLGTLL"
     gene            1809..3518
                     /locus_tag="VEC56_08080"
     CDS             1809..3518
                     /locus_tag="VEC56_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV14150.1"
                     /translation="MSHPRDTTSSTTKLNAELLAMVRIASSILQYSKLDDILTTITRE
                     LSPLIRFDRSSIALVAPDQQSLILHPIYAGPGEAGKLGEGRRLPLNEKTAIGWVVLNR
                     RAILRSDVATDGRFLDVVGDERLGCDMSVPLIAHGNVIGTLNLGCFEPDALVDSDLEK
                     LVNCGNIAGCAIEHAMLLEEAKDLGERYRTLQRDASDIIMLIDRNSGRLLEVNRQCCT
                     ALGFKEEDLLRKAYFDLFPPEDQFQARRDFVNIMSQKSRVFVDRRFVGRDGTIIFVDV
                     SASLVQIKSDTFIQVMVHDISQRKMLEQQIILQNRNLQDANQKLRQVDQMKTEFLANI
                     SHELRTPLSVIIAYTEAMRDGLVGEEDRRHFLDVISDNGDHLLRLINDLLDLSKLEVS
                     EAMLSFSLSHIHDVVRSLWPKVIEAAGQKRIEVAFTPGYEIPVVYIDNRRITQVFLCL
                     VQNAIKFTDAGGRVEVSTLRSEEGVVIQVRDTGAGISEEQIPHIFDTFRQLDGSSTRR
                     WGGLGIGLAIARHIVELHGGKIWVESKRGEGSTFAFTIPIETDEVLRNSGEPQTPARN
                     LRA"
     gene            complement(3572..>4784)
                     /locus_tag="VEC56_08085"
     CDS             complement(3572..>4784)
                     /locus_tag="VEC56_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14151.1"
                     /translation="DLNDALYNAGDVVYFYFSARSLNNVTSYYSQFTGMTTDQNEVRA
                     QPMEFTGLPSGGSNVLYVDYFSGYGAEPLFNSSFFQLGITPDRYDVRSPSSLGKNGPG
                     NHVFSQDQLFVYDTLVLNSGWFSDEVIAPDDWLLLAVFAEYNTNSPAGIYLSGDNIAA
                     MLATSQDPIASYLRSFYCDFSLISDTHRNIGLPVSPLVVPAPNSPFTGQFVAYGGAAS
                     PPGRFDVLKPGTNAVAAMTYEGDPGRAAAVVQSTVSPVTQGTVNFVLAGFSHHKIRDD
                     EPAGIPDRTVFLKDVLDAVTGSGGTPTTANRPVSRDALEQNVPNPFNPVTTIRYSIAR
                     AGHVNLVVYNAAGQRVASLVNEVQQPRAAGYAVTWDGHADSGARVASGVYFYKLTSPG
                     YSQTRKMVLLK"
BASE COUNT          931 a         1500 c         1538 g          815 t
ORIGIN      
        1 caggagtgtg ccgaggttgt aatcgacctc gcgctggatg agtcggtacg acggcgtcgg
       61 gtactgcccg ttgatctcgt cggcgcgcgt gtaatgcgag agggccgtgc ccttgctccc
      121 catcttgagg taggcgttgc ccagcgtgta gttcgaaaac gcgtcggagt acgcacgcat
      181 ctgcttctgg tagagatagt cggagttgac gagcgccgca gcggccgcca cgacgaggag
      241 cgccttcacc aagccgaact gtttctcgcg gaacgcgcgc cacacgtaca cgccgccgta
      301 ggcggcgaat aggcacagga tgggcacgat gggcaggcgg aagcgcgcgt tgacgaagaa
      361 ggcgatcacc cccaccgagt acaggccgac gaatagatac agcatcgaga ggtcgctgcg
      421 ccgtcgccac aacagaacgc cgcccaggat ggcgagcggc gccaccagcc aaaagcccgg
      481 cagcgggacg tacttcatct tggccaggtg ccagaagaag tagatgtact tatcgttcgc
      541 gcgctcgccc gcgccccaga acatctggaa cttcttcgcc atgtgcgcgg tcgcttcgct
      601 gggattcccg cggatgtagt cgaagccctt cttgaagtag tagtcggaca cctccgcgag
      661 cttgagcttc ttgcccacgt ccttctccgc catcgcgatg gcgtcctcgt agccgcccca
      721 ccagtcggcg cgggttcccg gcacgatcgc ggtcgagccg tccgacacgg ggttattgcc
      781 gatccagaaa ttgacgccgc ccgatgcgcc cacgggcacg accgccttcg cgacggcgta
      841 gttacgaatc atgacgggca ggatcgggat cgcgcacgca accgcgacca cgatcgactg
      901 cgcgacccac ccgcgccctt cgggccggcg cttccacgcg agccagagcg cgagcggtac
      961 ggcggggaag aacaccatga cgctgggtcg cgtgatggtt gcgatgccga gggcgaagcc
     1021 acccgccgcc aggagccaaa gccgccggcg ccggatgccc atggtcagga acagcagggt
     1081 gagcgaattg aagaagatga acagcgtctc caagaggaga tcgccttcca tgtacacgag
     1141 cacccaatag agggcgaccg cgaggcccgc gaccagcgag accgagcgcg agaacaattc
     1201 gcgcgcggtg agatacacca cgcccgcggt caacgcgccg atcaggtgct ggatgaacac
     1261 cgcgaaggcg atgctcgacc cgctcatctt gtagagggcg gccaggaagt acgggtacat
     1321 ggggccgcgg aaaaacgcgt cggtgcccgg tgtcccgccc gcgaccaagt ccaaggccca
     1381 ctcgtggtga tagagcgagt ccatcaccgg gaaataaaag gtcggattgt agatccggtt
     1441 gaggaagtag aacgcgacgc gcagcgccag cgccacaccc acgaccgtgc ccaactggag
     1501 ccaatccgcg cgattccccc aacggaacgg ctgggcggcg tcgagcgagt cgcgcaggtc
     1561 ggtgcgggcg ggcggggtag ggggctttcg ccgggcctgg acttgcttgt ctttcttggt
     1621 catacggagt gggcgaaggg gcagtctagc atacggttac gacgcgcaca atcgcgggcg
     1681 agtgatgcct ctttggaacg cttcgtgctg gatcggggtg ggtcgttgcg tcgccgggaa
     1741 gagggccttg tcgtccctta tgcggtacgg cgaggcgcga ccgggaatcc tcggttcgag
     1801 cgacgaccat gagccacccg cgcgacacca cctcgtccac gaccaaattg aacgccgagc
     1861 tcttggcgat ggttcgcatc gcgagttcga ttctgcagta ctccaagctc gacgacatcc
     1921 tgaccaccat cacgcgcgag ctctcgccgc tcattcggtt cgaccgctcc agcatcgcgc
     1981 tcgtcgcacc cgaccagcag tcgctgatcc tgcaccccat ctacgccggg ccgggtgaag
     2041 ccggcaagct gggcgaaggg cgccgccttc ccctcaacga gaagaccgcg atcggctggg
     2101 tagtcctgaa ccgccgcgcc atcctgcgca gcgacgtcgc caccgacggg cgcttcctcg
     2161 acgtggtcgg ggacgagcgc ctcggctgcg acatgtcggt gccgctgatc gcgcacggca
     2221 acgtgatcgg gacgttgaac ctgggctgct tcgaacccga cgcgctggtc gattccgatc
     2281 tcgagaagtt ggtcaattgc ggcaatatcg cgggctgcgc catcgaacac gcgatgctgc
     2341 tggaagaggc caaggatctg ggcgagcgct accgcacgct ccagcgcgac gccagcgaca
     2401 tcatcatgct gatcgaccgc aactcgggcc gcctgctcga agtcaaccgg cagtgctgca
     2461 ccgcactcgg cttcaaggaa gaagacctgc tgcgcaaggc gtacttcgac ctgtttccgc
     2521 ccgaggatca gttccaggcc cgccgcgact ttgtcaacat catgagccag aagtcgcgcg
     2581 tgtttgtcga ccgacgcttc gtcgggcgcg acggcaccat catcttcgtc gacgtcagcg
     2641 cgagcctggt ccagatcaag tcggacacgt tcatccaggt gatggtgcac gacatctcgc
     2701 agcgcaagat gctcgagcag cagatcatcc tccagaaccg gaatctccag gacgcgaacc
     2761 agaagctgcg ccaggtcgac caaatgaaga ccgagttctt ggccaacatc agccacgagc
     2821 tgcgcactcc gctctcggtc attatcgcgt acacggaagc gatgcgcgac gggctggtgg
     2881 gcgaagagga ccgccgccac ttcctcgacg tcatctccga taacggcgac cacctgctgc
     2941 ggctgatcaa cgacctgctc gatctatcga agctggaagt ctccgaggcc atgctctcct
     3001 tcagtctgtc gcacatccac gacgtggtgc gctccctgtg gccgaaggtg atcgaggcgg
     3061 cggggcagaa gcggatcgag gtcgcgttca cgcccggcta cgagataccg gtggtctaca
     3121 tcgacaatcg gcgcatcacg caggtgtttc tgtgcttggt tcagaacgcg atcaagttca
     3181 ccgacgccgg gggacgcgtg gaggtctcga cgctgcgcag cgaagaaggc gtggtcatcc
     3241 aggtgcggga caccggcgcc gggatctccg aggagcagat cccgcatatc ttcgatacgt
     3301 tccgccagct cgacgggtcg tcgacccggc gttggggcgg gctcggcatc gggctcgcga
     3361 tcgcgcgcca tatcgtcgag ctccacggag ggaagatctg ggtcgagagc aagcgcggcg
     3421 aaggcagcac cttcgccttc accatcccca tcgaaaccga cgaagtcctg cgcaactcgg
     3481 gcgagccgca aacgccggcg cgtaacctgc gcgcataaga aaaagggccg gcgatcgccg
     3541 gcccttcttc tatggcggcg tttacacaga gctacttgag taggaccatc tttcgagtct
     3601 gcgagtaccc cggagacgtg agcttgtaga agtacacgcc gctcgcgacg cgtgcgccgc
     3661 tgtccgcatg cccgtcccac gtcaccgcgt atccggctgc gcgcggctgc tgcacctcgt
     3721 tcaccaggct tgcgacgcgc tggccggccg cgttgtagac aacaaggttc acgtggccgg
     3781 cacgcgcaat cgagtagcgg attgtggtca ccgggttgaa cgggttgggt acgttctgtt
     3841 cgagcgcgtc gcgcgatacc ggccgattcg ccgtcgtcgg cgtccccccg cttccggtta
     3901 cggcatcgag aacatccttc aggaacaccg tacggtcggg gatgccggcg ggctcgtcgt
     3961 cacggatctt gtggtggctg aagcccgcca gtacaaagtt gaccgttccc tgcgttaccg
     4021 gtgaaaccgt ggactgcacg accgctgcgg cacgtccagg gtcgccttcg taggtcatcg
     4081 cggctaccgc gtttgttccc ggcttcagaa cgtcgaaccg acccggcgga gacgcggccc
     4141 cgccgtaagc gacgaactgg cccgtaaacg gggagttggg cgccggtacc acgagcggcg
     4201 acaccggtag gccgatgttg cggtgggtat ccgaaatcaa ggagaagtcg caatagaacg
     4261 atcgtagata gcttgcgata gggtcctgcg acgtcgccag catggcggcg atgttgtcgc
     4321 ccgaaaggta aatccccgcg ggtgagttgg tgttgtactc tgcgaagacc gcgagcaaca
     4381 accagtcgtc cggtgcgatc acttcatcgc tgaaccaccc gctgttgagt acgagggtgt
     4441 cgtacacgaa gagttgatcc tgggagaaga cgtggttccc gggtccgttc ttacccagag
     4501 aggatggcga acgcacgtcg tagcggtccg gcgtaatccc gagttggaag aacgaactgt
     4561 tgaacaatgg ctctgccccg tatccgctaa aatagtcgac atacagaacg ttagagccgc
     4621 cgctgggaag tcccgtgaac tccatcggct gcgcgcgaac ctcgttctgg tcggttgtca
     4681 tgccggtgaa ctggctgtaa tagctggtca cgttgttcag ggagcgcgcc gaaaagtaga
     4741 aatagaccac gtcgcccgca ttgtagagcg cgtcattgag atcg
//
LOCUS       DASPGK010000294         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_75450, whole genome shotgun sequence.
ACCESSION   DASPGK010000294 DASPGK010000000
VERSION     DASPGK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75450"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VEC56_08090"
     CDS             complement(<1..1329)
                     /locus_tag="VEC56_08090"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HXV14152.1"
                     /translation="MTTRVLVGTGGVLVAALVAIGLADQLHLRIPFFLASYGVAFAAY
                     LVAVRFVLRESSAPRSALVVMAVVTVAARVAVLPARPELSTDVYRYVWEGRVVLDGGN
                     PFATPPADSSLAHLRNQDFDLINHRPLTTIYPPLAQGLFALGAWIAPNVVALKSVFTL
                     FDLGIVLVLLALLRARGRPATHALVYAWSPLVIVETAHSGHLDAAGAFFLVLGVTLIV
                     TGRRVWGGVALGASFLVKYLAAAIVPFFARRGQRIALPILVATAMLGFLPFLDAGDGL
                     TRTLITYGSQWSFNGPPFMVLSVLLGEEARARLALTVLGAIVVLVAAMRERDIARYAF
                     IVIGAALLLSPTVYPWYLVWIVPFLCVFPNRAWIAFTGLIGISYWVWIVYDATGAWML
                     PNWALALEYVPFYGLLLWDAGRARESGRGGSHRGFPTRTSAASRDFQSGAS"
     gene            complement(1331..2788)
                     /locus_tag="VEC56_08095"
     CDS             complement(1331..2788)
                     /locus_tag="VEC56_08095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14153.1"
                     /translation="MERWDAILFAVYVALLGVLSIYGLHRYALLFLYLRHYKRRSHPR
                     PPAPERYPSVTVQLPLYNERYVARRVIEAAARIRYPRGLLRVQVLDDSIDDTGEIARA
                     TVAELRAAGVNAEYHHRATRSGFKAGALAVGLEATPDELVAIFDADFVPPEDFLEKTV
                     SYFNDPAVGMVQTRWGYLNRDYSLLTRVQALVLDGHFMLEHTARNYSGRYFNFNGTGG
                     VFRAAAIRDAGGWQGDTLTEDLDLSYRTQLRGWKFVFLPDVDCPSELPVDILGLKNQQ
                     YRWAKGSIQVGRKLLPSIWKSRAPLAVKLEATFHLSANLSYLLLVVLSLLMPLAVMRR
                     ARMASSGEFIVEALAFGLTTASVLIFYAVAQRELHRDWKPRLRDLPVILALGVGMCVN
                     NAWAVAEALAGRDTPFVRTAKYRIESLRDRWKSKLYRTARGRSFFVELGLCAYLATGF
                     AVIAGLGAWTALPYLGLFVAGYAYVFGLSVIHGRR"
     gene            complement(2804..>3695)
                     /gene="arsS"
                     /locus_tag="VEC56_08100"
     CDS             complement(2804..>3695)
                     /gene="arsS"
                     /locus_tag="VEC56_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HXV14154.1"
                     /translation="VQVNVGLTCNIACEHCHVSSSPRRTEQMSWETMEQVLRVARETG
                     AGVLDITGGAPEMNPHFRRFVATAAGHRLSVMVRTNLTILLEPGYVDLPEFFAAHRVR
                     LIASLPCYTKDNVDRQRGDGVYEGSIEAMRRLNALGYGRDSDLPLDLVYNPGGASLPG
                     DQASLEADYKRELWDRHGLVFTRLYTITNMPIGRFMTALKKENKASMYRDLLRSSFNE
                     ATIAPLMCRHQIDVDWTGVIYDCDFNLALRMPARCGDQGGPIHVRDFDARLHTRRIVT
                     GEHCFGCTAGHGSSCGGALA"
BASE COUNT          711 a         1234 c         1240 g          510 t
ORIGIN      
        1 gctcgctccc gattggaaat cacggctcgc tgcggaagtt cgcgtcggga accctcgatg
       61 cgagcctccc cgaccggact cgcgggctct gccagcatcc cacaacagaa gtccgtagaa
      121 cggcacatac tccaacgcca gcgcccagtt gggcagcatc cacgcgcccg tcgcgtcgta
      181 cacgatccac acccagtacg agattccgat caagcccgtg aacgcgatcc aggcgcgatt
      241 cggaaacacg cacaggaacg gcacgatcca taccaggtac cacgggtaca cggtgggcga
      301 gagcaagagc gcggcgccga tcacgatgaa cgcgtagcgc gcgatgtcgc gctcgcgcat
      361 ggccgcgaca aggacgacga tcgcgcccag caccgtcagc gccaggcgcg cgcgcgcttc
      421 ttcgcccagc agcaccgaca gcaccatgaa gggcgggccg ttgaacgacc actgggagcc
      481 gtacgtaatc agtgtgcgcg tgagcccgtc gcccgcgtcg agaaacggca gaaatccaag
      541 catcgccgtc gccacgagga tcggcaacgc gatccgttgg ccgcgccggg cgaagaacgg
      601 cacaatcgcg gccgcgaggt acttcaccag gaacgacgcc cccagcgcga ccccgcccca
      661 cacgcgccgg cccgtcacga tcagcgtcac gcccagcacc aggaagaacg cacccgcggc
      721 gtcgaggtgt ccggagtgcg cggtctcgac gatgaccagc ggactccacg cgtacaccaa
      781 cgcatgcgtc gctggacgcc cgcgcgcgcg caagagcgcg agcagcacca acacgatgcc
      841 gaggtcgaac aacgtaaaga cgctcttgag cgccaccacg ttgggagcga tccacgcccc
      901 cagcgcgaac aacccctgtg cgagcggcgg gtagatcgtg gtcagcggcc ggtgattgat
      961 gaggtcgaag tcctggttgc gaaggtgcgc gagcgacgag tcggcgggcg gagtggcgaa
     1021 cggattgccg ccgtcgagga ccacgcgtcc ttcccacacg tagcggtaca cgtcggtgga
     1081 aagctcgggg cgcgcgggta gcacggcgac gcgcgccgcc accgtgacca cggccatcac
     1141 gaccagcgcg gaacgcggcg cggacgactc ccgcaggacg aaacgaaccg cgaccagata
     1201 cgcggcgaac gcgacgccgt agcttgcgag gaagaacgga atccgaaggt ggagttggtc
     1261 ggcgagaccg atcgcgacga gtgctgcgac caacacgccg cccgtaccca cgagcacgcg
     1321 cgtcgtcatg tcagcggcgg ccgtggatca ccgagaggcc gaacacgtac gcgtagcccg
     1381 cgacgaagag gccgaggtac gggagcgcgg tccacgcgcc cagccccgcg atgacggcga
     1441 agcccgtcgc caggtacgcg cacagcccca gctcgacgaa gaacgatcgc ccgcgcgcgg
     1501 tgcgatagag cttgctcttc catcggtcgc gcagcgactc gatgcgatac ttggccgtgc
     1561 gcacgaaggg cgtgtcgcga ccggccagtg cttccgccac ggcccacgcg ttgttgacgc
     1621 acatgccgac tcccaaggcg aggatcaccg ggagatcgcg cagccgcggc ttccagtcgc
     1681 gatggagctc gcgttgggcc acggcgtaga agatcagcac agacgcggtg gtgagcccga
     1741 acgccagcgc ttcgacgatg aattccccgc tcgacgccat gcgcgcgcgc cgcatcaccg
     1801 cgagcggcat caggagcgag agcacgacca gcaacagata gctcaggttc gcgctcaagt
     1861 ggaaggtcgc ctccagcttg accgcgagcg gggcgcggct cttccagatg ctcggcagga
     1921 gcttgcgccc cacctggatc gagcccttcg cccagcggta ctgctggttc ttgaggccca
     1981 agatgtcgac cggcagctcg ctggggcaat cgacgtcggg aaggaagacg aacttccacc
     2041 cgcgcagctg ggttcgatag gaaaggtcga ggtcttcggt cagtgtgtcg ccctgccagc
     2101 cgcccgcgtc gcggatcgcc gccgcccgaa acaccccgcc cgtcccgttg aagttgaaat
     2161 agcggccgga gtagttgcgc gcggtgtgtt cgagcatgaa atgcccgtcc agaaccaggg
     2221 cctgcacgcg cgtgagcagc gagtagtcgc gattcaggta gccccagcgc gtctgcacca
     2281 tgccgaccgc ggggtcgttg aagtaggaca ccgttttctc gaggaaatct tccggcggga
     2341 cgaagtcggc gtcgaagatc gcgaccagct cgtccggcgt cgcctccagc ccgaccgcca
     2401 gcgcgccggc cttgaagccg gagcgtgtgg cgcggtggtg gtactccgcg ttgaccccgg
     2461 ccgcgcgcag ctccgcgacg gtcgcgcgcg cgatctcgcc cgtgtcgtcg atggagtcgt
     2521 cgagcacctg cacgcgcaag agcccgcgtg gatagcggat gcgggccgcg gcttcgatca
     2581 cgcgccgcgc gacgtagcgc tcattataga gcggaagctg gaccgtgacc gagggataac
     2641 gctccggagc gggcgggcgc ggatgcgagc gtcgcttgta gtggcgcagg tacaagaaaa
     2701 gcagcgcgta ccggtgcagc ccgtagatcg acagcacgcc caggagcgcg acgtacacgg
     2761 cgaaaaggat ggcgtcccag cgttccatcg gtaagcgagc ccgctacgcg agcgcacccc
     2821 cgcacgacga gccatgaccc gccgtgcacc cgaagcagtg ctcgccggtg acgatgcggc
     2881 gcgtgtgcag gcgagcgtcg aagtcgcgca cgtggatggg accgccttgg tcgccgcacc
     2941 gcgccggcat gcgcagcgcc aagttgaagt cgcagtcgta gatcacgccg gtccagtcga
     3001 cgtcgatctg gtggcggcac atgagcggcg cgatggtcgc ctcgttgaac gaggaacgca
     3061 ggagatcgcg gtacatcgac gctttgtttt ccttcttgag cgccgtcatg aaacgcccga
     3121 tgggcatgtt ggtgatggtg taaaggcgag tgaacaccag cccgtggcga tcccacaact
     3181 cacgtttgta gtccgcttcc agagacgcct gatcgcccgg cagactcgcc ccacccggat
     3241 tgtagaccaa atccagcggc agatcgctgt cgcgtccgta cccgagcgcg ttcaggcggc
     3301 gcatggcctc gatggaccct tcgtacacgc cgtcgccgcg ctgacggtcg acgttgtcct
     3361 tggtatagca cgggagagat gcaatcagac gcacgcggtg cgcggcgaag aactcgggca
     3421 gatcgacgta accgggctcg aggaggatgg tcaggttggt tcgtaccatc acggagagtc
     3481 tgtggcccgc ggccgtcgcg acgaagcgac ggaagtgagg gttcatctcc ggcgctccgc
     3541 ccgtgatatc gagcacgccg gcaccggtct cgcgcgcgac gcgcaacacc tgctccatcg
     3601 tctcccagct catctgctcc gtgcgacgcg gcgaggagga gacgtggcaa tgctcgcacg
     3661 cgatgttgca ggtgagcccg acgttgacct ggacg
//
LOCUS       DASPGK010000295         8966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_778366, whole genome shotgun sequence.
ACCESSION   DASPGK010000295 DASPGK010000000
VERSION     DASPGK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778366"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="fahA"
                     /locus_tag="VEC56_08105"
     CDS             <1..999
                     /gene="fahA"
                     /locus_tag="VEC56_08105"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HXV14155.1"
                     /translation="GSPALRENAAAAGELLVQRDDAELLMPVAIGDYTDFYASIYHAT
                     NVGMMFRPDTPLLPNYKHLPVGYHGRASSIVVSGTPIKRPRGQTKADDAAAPSFGPTT
                     MLDYELEMGAFIGGGNALGEPIAIQDARRSLFGLCLVNDWSARDVQRWEYQPLGPFLA
                     KNFGTTISPWVVTMDALAPFRVAPYRRDPGDPAPLPYLLDEEDQAVGGLDIVVEAWLE
                     SARMRDDGFEAVRVSRGNMKDLYWTLAQMVAHHTSNGCNLKPGDLIATGTISGQEKES
                     RGCLIERTWRGSEPLILPTDETRTFLQDGDTVILRAFCERDGVRIGFGECRGNITC"
     gene            1015..2439
                     /locus_tag="VEC56_08110"
     CDS             1015..2439
                     /locus_tag="VEC56_08110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV14156.1"
                     /translation="MSTNSAIHRDGQAIDIDPKHVDALRASLRGTLVTGGDAAYNDAR
                     SIWNAMIDRKPALIARCVGVSDIVAGVKFARETGIALSIKGGGHNIAGTAVADGGLMM
                     DLSNMRGVWVDSKARVAHAQPGALLGDIDRETQLHGQAAVLGFVSATGATGLTLGGGF
                     GYLSRRYGWTSDNVRSMDVVTADGRLIRASEKESADLFWGLRGGGGNFGVVTNIEYDL
                     YPVGPEIVGGGIAWHAESAPEVLAMLKSLTEQAPPELAIVGVLRIAPPAPWIDKSAHG
                     KPIVAMFVCHSGAPAEAEKFLAPVKAFGKPVGDILQRRPYVSQQSLLDATQPKGRRYY
                     WKSEYLPRLSTEVLEAGMTHWKKIVSPHSAVIFFPLGGAVAARASDHSAVGNRDAGYV
                     LNITASWEKPEDDGANVEWARSAWQDMRRFSTGGTYINFLTEEEAGQRIQDAYGKNYA
                     RLVAIKSKWDPENVFRMNKNILPV"
     gene            2572..3057
                     /locus_tag="VEC56_08115"
     CDS             2572..3057
                     /locus_tag="VEC56_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXV14157.1"
                     /translation="MAAQSNPQDAQLEYAGFWRRFVANFIDGALLAIIIWPILIAVYG
                     IEYFTSTDVVAGPIDFLLTWVLPTIAVVLFWMHKQATPGKMAVSARIVDATSGKKAST
                     GQLIGRYFAYIPSTLGLCLGFLWIAFDPKKQGWHDKLAGTVVVRPAKRGPAPVRFATP
                     S"
     gene            complement(3091..5421)
                     /locus_tag="VEC56_08120"
     CDS             complement(3091..5421)
                     /locus_tag="VEC56_08120"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14158.1"
                     /translation="MSARSRLLALLVVVLPAVVSSQPGGPLAFHGTPVAPLGGATLVI
                     SPVTGRLVVGNIGPGGLDGVELNTGPAQGVKIELPSSPAAPTGAEKVARIQSDDPIIT
                     PSIQWVHGPVGVDIFVDFSSTGADGHIIEICNDGMPVAQTFIPMGDVVSCNQVPIALD
                     VDMHAIPAIGVSPLVTGFEPAPMLMLTMPGPALFSRAAGPIATGNELRAYPSNPLAAA
                     NHMENLAILFDVPAVQAPDTVEIEDAAQLIEGRLHRGTMFGLIEKDFEGNVTFSVPAG
                     LTGGIEMEAKASDTAQSITQNLKFGNAGDTDVFYAMGQLTDGIRGTNDFVSGSCDGSS
                     WALAPDFSSSGSPSVSVQLFDAAGLPLGPPSPVVGEIVVGTNVYPTRTEASRKNGYVI
                     AYDYMDAVPVTISAGTFLANRIEMFGQGDVPALMTQTGRLFSSSSDFTIEIAGESNGV
                     LAPGGEFYLDSGFAGGFARNYDPIGCVEAVDVNPADGDPFVFEPVVQEDQFGVAWVPG
                     PSPSTQPGQSDWDFVSRLAGETGAIARMGIKMVPEGGGVKLYSLTRQTPKTDFGDRLK
                     AGTQTVAEFVHSDETVPFAILPAWPAAIGYQVLHYPDKTWSMWINLGQMMDVAIPDPP
                     AGMSATQVVSADFIEVVFLDTPIASPVAESKLVVRGYTKSHRLFYGTKSKAVSVGRAP
                     SRDGLSLRAPYPNPFNPSTTLSFDIPTAGDVSIRVYTVRGEYVATLHSGWMGPGPHAV
                     SWDGVNHRGQRAASGVYLAELRAPGGTRHVKLNLLK"
     gene            5577..7184
                     /locus_tag="VEC56_08125"
     CDS             5577..7184
                     /locus_tag="VEC56_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14159.1"
                     /translation="MNTHRRPARITSVFVSIALVMLNSSTAAAQQTLDFQAVLLPLPT
                     GATASEARGIAERGWEPLTIAGAAETSTQNKNASCWIRVSNVWSAHMLPGLSPTGDSW
                     ANAVDHAPAAQGEWTLVVGAAMDESGVQRPMRWDNVKNQPWTGTPLPTPPGGSGEVFS
                     VYLLFKPDGTPLRARACGWVSEAPPTVPSGGATQSVPTGAKPAFMPIGIGGSNLIYPE
                     FPAGLDGVMYDVSSSDNDVLMGVGGGENAVGQWRPQLWTSLDDGQTWSNEELPLPLGV
                     PSGEVTDVGHEVGHWLGAGWGMSTAGATVPLLWEMNTNDPIPTWIVHELPLPPLDEGG
                     QNGFVRKRPGRTMYANIVLKRGLDPVVGIWVDELGGGWSLYEPDDYLINPEIGVPVAP
                     AGIDKYGRIAVKFPNPPGSTETRNGAAPMGTTAGLLIPSPETGVGDGASPQRLIALTA
                     SPNPFQSGVRIGFTLPWDASVKVSIHDVSGRVVASFDRGQRAAGDEHVLRWDGIADDG
                     QRAASGVYFVRVETPYAKSTLKLVRIE"
     gene            7262..8524
                     /locus_tag="VEC56_08130"
     CDS             7262..8524
                     /locus_tag="VEC56_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV14160.1"
                     /translation="MSARYFATLILPVLFCGCAASVDVDSMLRDYDDYEKDVRAVATD
                     PHAIQTLDAAQAKRTEAGALAEKGDKNKAVPKVEEAMADARLALEVDQAMASARRAEK
                     CRLEVEQSRKKWSEAVFVLEQTEEFVGKDAPTAKLEPETAPAEAALPASTLTPDSFPP
                     ASMNDVAAQWTAWRKAASDSKVAVAELEGAYRKSFDATQAAKVDAATEAHNRYLAARA
                     VQSLECRVRDGASEQVCVDATAQMAAYGDAREGALRATLELERTLQDDLRRDLDQLRA
                     EAKTRQDELFDALSQMEGKFASIHRDARGTIVSLADILFDFDKATLRRDVEFNLVKIA
                     TILNQFGEMKILIEGHTDAIGTDEYNLGLSKRRAQAVHDFLVSQDVAATRLSWDGYGE
                     SRPVADNTTDEGRQKNRRVDLVIQDAPQ"
     gene            8521..>8966
                     /locus_tag="VEC56_08135"
     CDS             8521..>8966
                     /locus_tag="VEC56_08135"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXV14161.1"
                     /translation="MKLVFHGHSFWEIVGEKHRLLIDPFITGNPLADVRPQEFKQLDA
                     ILISHGHGDHLGDAETIAKKTGALIVANFEIAGYYMDRGCKAHGMSIGGGHDFPFGRV
                     KLVIAHHGSTGPNGEALGSPAGIVIHMDGKRVYHTGDTGVFLDMQL"
BASE COUNT         1598 a         2845 c         3022 g         1501 t
ORIGIN      
        1 ggcagtcccg cgttgcgcga gaacgcagcg gccgcaggcg agctgctggt gcagcgcgac
       61 gacgccgagc tgctcatgcc cgtcgcgatc ggcgactaca ccgacttcta cgcgtcgatc
      121 tatcacgcga ccaacgtcgg aatgatgttt cgacccgaca cgccgctctt gcccaactac
      181 aagcaccttc ccgttggcta ccacgggcgc gcgtcgtcga tcgtcgtgtc cgggaccccg
      241 atcaagcgcc cgcgcgggca gaccaaggcc gacgacgcgg ccgcgccgtc tttcgggccc
      301 acgacgatgc tcgactacga gctcgagatg ggggccttca tcggcggcgg taacgcgctc
      361 ggtgagccga tcgcgatcca ggacgcgcgt cgcagtctgt ttgggctgtg cttggtcaac
      421 gattggtccg cgcgcgacgt ccaacgctgg gagtaccagc cgctggggcc tttcctggcc
      481 aagaacttcg gcaccaccat ctcgccgtgg gtcgtgacca tggatgcgtt ggcgccgttc
      541 agagtcgcgc cgtaccggcg cgacccagga gatcctgcac ccctcccgta cttgctcgat
      601 gaggaagatc aagcggtggg agggctcgat attgtcgtag aagcgtggct cgaaagcgcg
      661 cgcatgcgcg acgacggctt cgaagccgtg cgcgtctcgc gcggcaatat gaaggacttg
      721 tattggacgc tcgcgcagat ggtggcgcac cacacgtcga acgggtgcaa tctcaagcca
      781 ggcgacctga tcgcgaccgg gaccatctcc ggacaagaga aggaatcgcg cgggtgtttg
      841 atcgagcgga cgtggcgggg gagcgagccg ttgatactcc ccaccgacga gacgcggacg
      901 ttcttgcagg acggcgatac cgtcatactg cgggcgttct gcgaacgaga cggcgtacgg
      961 atcggattcg gcgagtgccg aggcaacatc acctgttagt tagaaggaga ggacatgagc
     1021 accaattcgg cgatccatcg agacggccag gcgattgaca tcgaccccaa gcacgtcgac
     1081 gcactgcggg cttcgctgcg cggcacgctg gtaaccgggg gagacgcggc ctacaacgac
     1141 gcgcgttcga tttggaacgc gatgatcgac cgcaagcccg cgctcatcgc gcgctgcgtc
     1201 ggggtgtccg acatcgtggc gggcgtcaaa ttcgcgcgtg agaccggcat cgccctctcc
     1261 atcaagggag gcgggcacaa tatcgccggg accgccgtcg cagacggcgg ccttatgatg
     1321 gatctctcga atatgcgcgg cgtgtgggtc gactccaagg cgcgcgtagc gcacgcgcag
     1381 cccggagcgc tcctgggcga catcgatcgc gagacgcagc tccacggaca ggccgcggtg
     1441 ctcggtttcg tctccgcgac cggtgcgacc gggctgacgc tgggcggagg attcggatac
     1501 ctctcgcgac ggtatggatg gaccagcgac aacgtgcgct ccatggacgt cgtcaccgcg
     1561 gacgggcgcc tcatacgcgc cagcgagaag gaaagcgccg acttgttctg ggggctgcgg
     1621 ggagggggcg ggaacttcgg cgtcgtcacc aacatcgagt acgacctgta tcccgtcggt
     1681 cccgagatcg tgggtggcgg gatcgcgtgg cacgccgagt ccgcgcccga ggtgctcgcg
     1741 atgctcaagt cgctgaccga acaggccccg cccgagctcg cgatcgtggg cgtgctgcgc
     1801 atcgcgccgc ccgcgccctg gatcgacaag tccgcgcacg gaaagcccat cgtcgcgatg
     1861 ttcgtgtgcc actccggcgc gcccgcggaa gcggagaagt tcctggcacc cgtaaaggcg
     1921 ttcgggaagc cggtgggcga cattctgcaa cggcggccgt acgtgtcgca gcagagcttg
     1981 ctcgacgcga cccagcccaa gggccggcgt tactactgga agtcggagta tctcccgcgc
     2041 ctgtcgacgg aggtgttgga ggcggggatg acgcactgga agaagatcgt ctccccgcat
     2101 tcggccgtca tcttctttcc gttgggtggg gcggtcgccg ctcgcgcgag cgaccactcg
     2161 gcggtgggga atcgggatgc cggctacgtg ctcaatatta cggcctcgtg ggagaaaccc
     2221 gaggacgacg gcgccaacgt cgaatgggcg cgcagcgcct ggcaagacat gcgccgcttt
     2281 tcgaccggcg ggacgtatat caatttcttg acggaagaag aggccggcca gcgcatccag
     2341 gacgcctacg gcaagaacta cgcgcgcctc gtcgccatca agtcgaaatg ggatccggag
     2401 aacgtgttcc ggatgaacaa gaacattttg ccggtgtgag ggtgatcggc cgagccgggc
     2461 agccaagggt taccttcggg aacgctatat gcaccgtatt cgagtttcgg tgctaggaag
     2521 tggggcggta ttcccactgt tcgaggcctt gtcagctgca ggaggggaac catggctgca
     2581 caaagcaacc ctcaggatgc ccaactcgag tacgcgggtt tctggcgacg cttcgtcgcg
     2641 aactttatcg acggagcact cctggcgatc atcatttggc cgattctcat cgcggtctac
     2701 ggcattgagt atttcacttc gacggacgtc gtcgcgggac cgatcgattt ccttctgacg
     2761 tgggtgctgc cgacgatcgc cgtcgttctg ttttggatgc acaagcaggc gactcccggc
     2821 aagatggccg tgtccgcccg tattgtcgat gccacttcgg ggaagaaggc atccaccggg
     2881 cagctcatcg ggcgatactt cgcgtacatt ccgtcgacgc tgggcctttg tctcggcttc
     2941 ctgtggatcg cgttcgatcc gaagaagcag ggctggcacg acaagctcgc gggcacggtc
     3001 gtggtgcggc cggccaagcg cgggcccgcg ccggttcggt tcgcgacgcc gagttagtga
     3061 gcagtggggc gacttagctc gccccgcggc ctacttcagc aggttcagct tcacgtgccg
     3121 cgttccgccc ggtgcgcgca gctcggccaa gtacaccccg gacgccgcgc gctggccgcg
     3181 gtggttgacg ccgtcccagc tcaccgcgtg cggccccggt cccatccacc ccgaatgcag
     3241 cgttgccacg tactcgccgc gtaccgtgta gacgcgaatc gacacgtcgc cggcggtcgg
     3301 gatgtcgaac gacagcgtcg tcgacggatt gaagggattc ggatacgggg cgcgcagcga
     3361 cagtccgtcg cgcgacggcg cgcgtcccac ggagacggcc ttcgacttgg ttccgtagaa
     3421 gagccggtgt gacttggtgt agccgcgcac gacgagcttc gactccgcca cgggactcgc
     3481 gatgggcgtg tcgaggaaaa ccacttcgat gaagtccgcc gagacgactt gggtcgccga
     3541 catgccggcg ggcggatcgg ggatcgcgac gtccatcatc tgcccgagat tgatccacat
     3601 cgaccacgtc ttgtcgggat agtggagcac ctgatagccg atggccgcgg gccacgcggg
     3661 caagatcgcg aacggcacgg tctcgtcgga gtggacgaac tcggcgacgg tttgcgtgcc
     3721 cgccttcagg cggtcgccga agtcggtctt gggggtctgg cgcgtcaacg aatacagctt
     3781 cacgcccccg ccctcgggca ccatcttgat tcccatgcgc gcgatcgcac ccgtctctcc
     3841 ggccaggcgg ctgacgaagt cccaatcgct ctgcccgggc tgcgtgctag gcgacggacc
     3901 cggcacccac gcgacgccga actggtcttc ttgtacgacc ggttcgaaga cgaaggggtc
     3961 gccgtcggcg ggattcacgt cgaccgcttc cacgcaacca atcgggtcgt agtttcgtgc
     4021 aaaccctcct gcgaaccccg agtcgaggta gaactctccc ccgggcgcga gcacgccgtt
     4081 ggactccccg gcgatctcga tggtgaaatc cgacgagctc gagaacagac gtcccgtttg
     4141 cgtcatcaat gcgggaacgt cgccttgccc gaacatctca atccggttgg cgagaaacgt
     4201 ccccgcggaa atcgtcacgg gaacggcgtc catatagtcg tacgcgatca cgtacccgtt
     4261 cttgcgactg gcttcggtcc gcgtggggta cacgttggtg ccgacgacga tctcgcccac
     4321 gaccggactg ggaggcccga gcggcagccc ggccgcgtcg aagagctgca cgctcacgct
     4381 cggtgacccg gagctgctga agtcgggggc cagcgcccac gacgaaccgt cgcacgatcc
     4441 ggacacgaag tcgttggtac cgcgtatgcc atccgtcagc tggcccatgg cgtagaacac
     4501 gtcggtgtct ccggcgtttc cgaacttgag gttctgggtg atgctctgcg cggtatccga
     4561 cgcctttgct tccatctcga tgccgcccgt gagtccggcg ggaaccgaga acgtgacatt
     4621 accctcgaag tctttctcga tcaggccgaa catggtcccg cgatggaggc ggccttcgat
     4681 gagctgggcc gcgtcctcga tctccacggt gtccggcgcc tggactgcgg gcacgtcgaa
     4741 gaggatcgcg aggttctcca tatggttcgc ggccgcgagc ggattcgacg ggtaggcgcg
     4801 cagctcgttg ccggtcgcga tcggtccggc ggcacggctg aacaaggccg gcccgggcat
     4861 agtcagcatg agcatcggcg cgggttcgaa cccggtgacc agcggcgaga cgccgatggc
     4921 cggaatcgcg tgcatatcaa cgtcgagcgc gatgggcacc tggttgcacg acaccacgtc
     4981 tcccatcggg atgaacgtct gcgcgaccgg catgccgtcg ttgcagatct cgatgatgtg
     5041 gccgtccgcc ccggtggaac tgaagtcgac gaaaatatcc accccgacgg ggccgtgcac
     5101 ccactgaatg ctcggtgtga tgatgggatc gtcggactga atccgcgcga ccttctcggc
     5161 gccggtgggc gcggctggcg acgacggaag ctcgatcttg accccttgcg cggggcccgt
     5221 gttcagctcg acgccgtcca ggccgccggg accgatgttg cccacgacga gtcggcccgt
     5281 cacgggcgat atgaccagcg tcgcgccacc gaggggcgcg accggggtgc cgtggaatgc
     5341 cagcgggcct ccgggttgcg aggacacgac agccggaaga acgacgacga ggagcgcgag
     5401 caatcgcgag cgagcggaca tgagggacct ccgagggtcg aaggcgtcat gggttcgggg
     5461 cccgtgggac ttgttgaccg tcagtttaac acaggtcgag atcctgtgct aaactcactg
     5521 ccgcacgtcc gcgtgccgcc aagggcctca ctgtccaccc cggctcggag gttaccatga
     5581 acacgcatcg ccgtcccgcc cggatcacgt ccgtcttcgt gtcgattgcc ctcgtgatgc
     5641 tcaattcgtc gaccgccgcg gcccagcaaa cgctggactt ccaagccgtt cttcttccgc
     5701 ttccaacggg agcgaccgcg agcgaagcgc gcggcatcgc cgagcgcgga tgggaaccgc
     5761 tcacgattgc gggggctgcg gagacctcga cgcaaaataa gaacgcgtcg tgctggattc
     5821 gtgtctcgaa cgtgtggagc gcgcacatgc tgcccgggct ctcgccgacc ggtgacagtt
     5881 gggcgaacgc ggtcgaccac gcgccggccg cgcaaggcga gtggaccctc gtcgtgggtg
     5941 cggcgatgga cgagagcggc gtgcagcgcc cgatgcgatg ggacaacgtg aagaaccagc
     6001 catggacggg aacaccgctc cccacaccgc ccggtggcag cggcgaagtg ttctccgtgt
     6061 atctcttgtt caagcccgat ggaacgccat tgcgcgcccg cgcctgcggc tgggtcagcg
     6121 aagctccgcc caccgtgccc agcggcgggg cgacgcagtc ggtcccgacg ggggcgaagc
     6181 cggccttcat gccgatcggc atcggcggct ctaatctgat ctacccggaa ttccccgcag
     6241 ggctcgacgg cgtcatgtat gatgtgtcgt cctcggacaa cgacgtcctc atgggtgtgg
     6301 gcggcggcga gaacgcggtg ggtcaatggc ggccgcaact gtggacatcg ctggacgacg
     6361 ggcaaacgtg gtcgaacgaa gaattgccac taccgctggg cgtgcccagc ggcgaggtca
     6421 cggacgtcgg ccatgaagta gggcattggt tgggcgcggg gtggggaatg tcgaccgcgg
     6481 gtgccacggt gccgctcttg tgggagatga acacgaacga tcctattccg acctggatcg
     6541 tccacgagtt gccgctgcca ccactcgacg aaggcggcca gaatggtttt gtccgcaagc
     6601 gcccgggtcg tacgatgtac gccaatatcg tgttgaagcg tggactcgac cccgtggtcg
     6661 gcatctgggt cgacgagctc ggcggcggtt ggtcgctcta cgagcccgat gactatttga
     6721 tcaatccgga gatcggagta ccggtcgcac cggcggggat cgacaagtac gggcgcatcg
     6781 cggtcaagtt cccgaatccg cccgggtcga cggagacgcg caatggggcc gcgccgatgg
     6841 gaaccaccgc ggggctcttg attccctcgc cggaaaccgg cgtcggcgac ggcgcatctc
     6901 cgcagcgcct gatcgctctc acagcgtctc cgaatccgtt ccagtcggga gttcgcatcg
     6961 gcttcacgct gccgtgggac gcgtcggtga aggtgtcgat tcacgacgtg tcgggtcgcg
     7021 tagtggcgtc cttcgatcgc gggcagcgcg cggcgggaga cgagcacgtg ttgcgctggg
     7081 acggtatcgc ggacgacggc cagcgcgcgg cctcgggcgt ctacttcgtg cgcgtggaga
     7141 cgccctacgc gaaatcgacg ttgaagctcg tgcggatcga gtaggcgtct ctgtttttgc
     7201 ttgccacccc gccttcgatg gtcaaacctt gtcggcaacg gtttcgacca aaggagtacc
     7261 catgagcgca cgttacttcg ccaccctgat cctccccgtc ctcttctgcg gatgcgccgc
     7321 gagcgtggac gtggactcga tgctccgcga ctacgacgac tacgagaaag acgtgcgcgc
     7381 ggtcgcgacc gatccgcacg cgattcagac cttggacgcc gcgcaagcga agcgcaccga
     7441 ggccggcgct ctagccgaga agggagacaa gaacaaagcc gtcccgaaag tcgaagaggc
     7501 catggccgat gcgcgcctgg cgctcgaggt cgaccaggcg atggcgtcgg cgcggcgcgc
     7561 ggagaagtgc cgcctggaag tcgagcagtc ccgcaagaag tggagcgagg ccgtcttcgt
     7621 gcttgagcag acggaagaat tcgtgggcaa ggacgcgccc acggcgaagc tggagccgga
     7681 aacggcgccg gcggaggcgg ctcttccggc ttcgaccttg acgcctgatt cctttccgcc
     7741 cgcatcgatg aacgacgtcg cggcgcaatg gacggcatgg cgcaaggcgg catcggatag
     7801 caaggtcgcg gtcgcggaac tcgagggcgc gtaccggaag agcttcgacg cgacccaagc
     7861 ggccaaggtg gatgcggcca cggaggccca taaccggtac ctcgccgcgc gcgccgtcca
     7921 gtcgctggag tgccgcgtgc gcgatggggc aagcgagcag gtgtgcgtcg atgcgacggc
     7981 gcagatggcc gcctacggcg acgcgcgcga aggggcgctg cgcgccacac tggagctcga
     8041 gcgcacgctc caggacgacc tgcgtcgcga cctcgatcag ctgcgcgcgg aagcgaagac
     8101 gcggcaggac gagctcttcg acgccttgag tcagatggaa gggaagttcg cgagcatcca
     8161 tcgtgatgct cgcggtacca tcgtgagctt ggccgatatt ctcttcgact tcgacaaggc
     8221 gacgttgcgc cgcgacgtcg agttcaacct ggtcaagatc gcgaccatct tgaatcaatt
     8281 cggagagatg aagatcttga tcgaaggcca caccgatgcc atcggcaccg acgagtacaa
     8341 cctcggcctc tcgaagcggc gcgcgcaggc ggtccacgac ttcctggtct cgcaggacgt
     8401 cgcggcgacc cgcttgagct gggacggcta cggcgaatcg cgcccggtcg ccgacaacac
     8461 caccgacgaa ggccggcaga agaaccgccg cgtcgacctc gtcatccagg acgcgccgca
     8521 atgaagctcg tcttccacgg tcattcgttc tgggagatcg tcggcgagaa acatcgcctc
     8581 ctgatcgatc ccttcatcac cggcaacccc ctcgccgacg ttcgtccgca ggagttcaag
     8641 cagctcgacg cgattttgat ctctcacggc cacggcgacc acttgggcga cgcggagacc
     8701 atcgcgaaga agacaggcgc cttgatcgtc gccaacttcg agatcgcggg atactacatg
     8761 gaccgcggct gcaaggcgca cggaatgagc atcggcggcg gtcacgactt tcccttcggg
     8821 cgcgtgaaac tcgtcatcgc gcaccacggc tcgaccggtc ccaacggcga agcgctcggg
     8881 agcccggccg ggatcgtgat ccacatggac ggcaagcgcg tctatcacac gggggacacg
     8941 ggcgtattcc tcgacatgca attgat
//
LOCUS       DASPGK010000296         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_778414, whole genome shotgun sequence.
ACCESSION   DASPGK010000296 DASPGK010000000
VERSION     DASPGK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778414"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VEC56_08140"
     CDS             <1..209
                     /locus_tag="VEC56_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HXV14162.1"
                     /translation="NGMTYRGVRQQFVKLGPARGDQVAVVHGLKAGEEVVTSGVFKLR
                     PGAAVQVNNSVTPSNDPTPQPEDS"
     gene            210..>2001
                     /locus_tag="VEC56_08145"
     CDS             210..>2001
                     /locus_tag="VEC56_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV14163.1"
                     /translation="MKITDLFVRRPVLAVVVNLVILIAGLQSIRSLSVRQYPRSDIAV
                     VRVTTAYIGANADLVRGFITTPLERVIASADGIDYIESSSAQSMSTITVHLKLNYDTN
                     AALTQIQAKVAQVRNDLPPEAEAPIIELETADNQFASMYLGFSSTDLDQNQITDYLTR
                     VVQPKLSAISGVQRADILGDRTFAMRVWLKPDQMAALGIAPADVHDALADNNYLSALG
                     ATKGSMVSVNLVANTDLTTVEEFEQLVVKEENGVVVRLGEIADVVLGAENYEEDVRFN
                     GETATFMGIWVLPNANSLEVIARVRDAMPEIQSQLPAGMKAGIPYDSTKYIEDAIHEV
                     LETLSETLLIVILVIFLFLGSVRSVIIPVIAIPISLVGAVFLMLVAGFTINLLTLLAI
                     VLSVGLVVDDAIVMVENVERHLHMGKKPFDAAIHAARELVGPIVAMTITLAAVYTPVG
                     IQGGLTGALFREFAFALAGAVIVSGVVALTLSPMMGSKLLRAGDTEKGFAGFVNRRFD
                     AVRRRYTRALAGTLQYRRLVYVLWGVAAFLMVPFYLFSQRELAPNEDQGVVFGIVQAS
                     ANATIDQTKLFANEVQKVYAAFPETENTFQL"
BASE COUNT          348 a          675 c          624 g          354 t
ORIGIN      
        1 ccaacggcat gacgtaccgg ggcgtgcgcc agcagttcgt caagctcggt cccgcgcggg
       61 gcgatcaagt ggcggtcgtg cacggcttga aagccggcga ggaagtggtg acgtcgggcg
      121 tgttcaagct gcgccccggc gcggcggtgc aggtcaacaa ctcggtcacg ccttccaacg
      181 atccgacgcc ccagccggag gacagttaaa tgaagatcac cgacctgttc gttcgccggc
      241 ccgtcctcgc ggtcgtcgtc aacctcgtca ttttgatcgc ggggttgcag tcgatccgct
      301 ccctgagcgt gcgccagtac ccgcgcagcg acatcgcggt ggtgcgcgtg acgacggctt
      361 acatcggcgc caacgccgac ttggtccgcg gcttcatcac gaccccgctc gagcgcgtga
      421 tcgcgagcgc ggatggcatc gactacatcg aatcgtcgag cgcgcagagc atgagcacca
      481 tcacggtgca cttgaagctc aactacgata ccaacgcggc gctgacgcaa attcaggcca
      541 aggtggcgca ggtgcgcaac gacctgcccc ccgaggcgga agcgcccatc atcgaattgg
      601 agacggcgga caaccagttc gcctccatgt atctcggttt ctcgtcgacc gacctcgacc
      661 agaaccaaat caccgactat ctcacgcgcg tagtgcagcc caagctctcc gctatcagcg
      721 gcgtgcagcg cgccgacatc ctcggcgatc gcaccttcgc gatgcgcgtg tggctcaagc
      781 ccgaccagat ggcggcgctc ggtatcgcgc ccgcggacgt ccacgacgca ctcgccgaca
      841 acaactatct ctcggccctg ggcgccacca agggctccat ggtgtcggtg aacctggtcg
      901 cgaacaccga cctcaccacg gtcgaagagt tcgagcagct cgtcgtcaaa gaggagaacg
      961 gcgtcgtcgt acgcctggga gaaatcgccg acgtcgtgct gggcgccgag aactacgagg
     1021 aagacgtccg cttcaacggc gagaccgcga ccttcatggg catctgggtc cttcccaacg
     1081 ccaactcgct ggaggtcatc gcgcgcgtgc gcgacgccat gccggagatc caatcccagc
     1141 tgcccgcggg catgaaggcc ggcattccct acgactccac caagtacatc gaagacgcga
     1201 tccacgaggt gctggaaacc ctctcggaga cgctcttgat cgtgatcctc gtcatcttcc
     1261 tgttcctggg ttcggtgcgc tcggtgatca tccccgtcat cgcgattccc atctcgctgg
     1321 tgggcgccgt gttcctgatg ctcgtggccg gcttcaccat caacctgctg actctgctcg
     1381 ccatcgtgct ctcggtgggg ctcgtggtcg acgacgccat cgtcatggtc gagaacgtcg
     1441 agcgccacct gcacatgggg aagaaaccgt tcgacgccgc catccacgcc gcgcgcgagc
     1501 tggtggggcc gatcgtggcc atgactatca cgctggccgc ggtgtacacg ccggtcggca
     1561 tccagggtgg gctgacgggt gccttgttcc gcgagttcgc gttcgcgctc gcgggcgcgg
     1621 tcatcgtctc gggcgtggtg gcgctgaccc tctcgcccat gatggggtcg aagctcctgc
     1681 gcgccggcga caccgagaag ggcttcgcgg gcttcgtcaa ccggcgcttc gacgccgtgc
     1741 ggcgccgtta cacgcgcgcg ctcgcgggaa cactccagta ccgccgcctc gtgtacgtgc
     1801 tgtggggtgt ggccgcgttc ctgatggtgc cgttctatct gttctcgcag cgcgagctcg
     1861 cgcccaacga agaccaggga gtggtgttcg gcatcgtgca ggcgtccgcc aacgccacca
     1921 tcgaccagac caagctcttc gcgaacgaag ttcagaaggt ctacgcggca tttcccgaga
     1981 ccgagaacac gttccagttg a
//
LOCUS       DASPGK010000297         8867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_661414, whole genome shotgun sequence.
ACCESSION   DASPGK010000297 DASPGK010000000
VERSION     DASPGK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661414"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..416
                     /locus_tag="VEC56_08150"
     CDS             159..416
                     /locus_tag="VEC56_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14164.1"
                     /translation="MHLHRSIALVATVAALACGKGEQASEPAKEHVVFGEFIAPEDSF
                     PKIRYYEAGLVSPNDRCAVRKVRLNVKMPPVYVNGRPIGFC"
     gene            438..788
                     /locus_tag="VEC56_08155"
     CDS             438..788
                     /locus_tag="VEC56_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14165.1"
                     /translation="MQGPEPYLSDFHIQLPCAVDTTALAVLDPAHRAFVNHEIYFFST
                     DLAREQFEAEPWRYTGKVTDPVTVVRFRPDASSPSRAAAGRLFYFESAETVARFDEDP
                     ARYSTPKPTMHELE"
     gene            1151..2260
                     /locus_tag="VEC56_08160"
     CDS             1151..2260
                     /locus_tag="VEC56_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV14166.1"
                     /translation="MTWSVTRGTITPLNAAGTLVQWTVPLEVGVDSVTASANDGTTSR
                     SVVTEIKVGTAFSGAIAPARFEQARSPYIVTVTGFSPVLAVTGRVTVIEPGTELLLET
                     ANTVIDVTDSLIAVGTPGAPIVIRPNVRNLTCGDDRGWWEGVKVYSDAPSHGYLELDY
                     ADLSYGRFSVRLRDQGSAVIRNSAIRCSGQNGVLHEGGGVLLLEDTQVTDGEFDGVAV
                     DSDTFQPDSILVSNCRINFNGRTGVMLRLNDPLQDTPILIEYSEVRFNAEHGITLENA
                     VFPEIHFNSFFGNGAGSTSGLNSIWLVNGYPGGASVTEVDATCNFWGAPVTNQSVIDA
                     VVRDNTDTGAVGARVVTSPWSNENPLVTPSTCVVP"
     gene            2289..3053
                     /locus_tag="VEC56_08165"
     CDS             2289..3053
                     /locus_tag="VEC56_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV14167.1"
                     /translation="MGKIIAIANQKGGVGKTTTAVNLCASLAAAERKTLLVDMDPQAN
                     AVSGVGIDKALIQQTIYEVLFDDNLVLDAILESPMPFLHVLPAKPSLAGAEVELVAEP
                     RREFRLKRALAHIRDEYDYIVVDCPPSLGFLTVNTLTAADSLLIPLQCEYYALEGLTQ
                     LLSTVKMVQASLNPDLAIEGVLLTMYDKRLNLASQVAQEAMNYFGEKVYKTVIPRNVK
                     LSESPSFGKPIILYDVQCQGARSYLDLAREVIGDVH"
     gene            3043..3915
                     /locus_tag="VEC56_08170"
     CDS             3043..3915
                     /locus_tag="VEC56_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXV14168.1"
                     /translation="MSTRKVLGRGLEALIPSAPMIEQAQPGERVVDVPTDAVDANPFQ
                     PRKRFDDDRLKELAESIRTDGVLQPVVVRRKGAKYELIMGERRLQAARLAGVPTIPVV
                     VRDVRDADALRLAIVENIQRENLNAIEEAHAFRRLISEFGLSQADVAGLVGKDRSSVA
                     NTLRLLNLSEEVQRMVEDGILTGGHARALLSLPTQKEQLALARRIAEQNLSVRQVEAE
                     VGLTRVKKSNAARRHKEKPAYLADLEKAFAQYLGTRVSIEEKRGGKGRIQVEFYSYDD
                     FERLAGLMQIPLPR"
     gene            3928..4728
                     /locus_tag="VEC56_08175"
     CDS             3928..4728
                     /locus_tag="VEC56_08175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV14169.1"
                     /translation="MAKEFSVIIVPHDHRTTWNLRVSYRMALLFGILAGVSVISGIAF
                     VITYGKIATAANRSLLLGRDNERMRAELAQVDSLKVELLRMQALGIQFKGMLGVPLSP
                     EDSVLVASLSPVAKTPAIPSDEEEGGVGPGEQRLMLDSMPSMWPVKGYVTREFSVTGG
                     EKSPDYHPGMDIAAARNTPVVVAADGIVEASAFDDVYGWMVEVDHGYGIRSVYGHNSR
                     NLVNIGDRVTRGKTIAFVGSTGKSTAPHLHFEVRENGVPVDPRKYLLN"
     gene            4820..5287
                     /locus_tag="VEC56_08180"
     CDS             4820..5287
                     /locus_tag="VEC56_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXV14170.1"
                     /translation="MFGKDAEITTGQSTLNSMLGQGCKIKGDIELQGTIRIDGQFEGS
                     ISCPDTLIIGKSGVVKADVKVKNAVIGGKLVGNISASNKIELQTGSHVEGDIATARLV
                     IDEGVFFEGNCKMGATGTGTSQSRDVSDHSKDSNWARKDEKRPEPVAPIGVTR"
     gene            complement(5662..6546)
                     /locus_tag="VEC56_08185"
     CDS             complement(5662..6546)
                     /locus_tag="VEC56_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14171.1"
                     /translation="MLPRPATMITAVVVTFAIAGVMKLSTRGMTSAAPDPEGLRDETP
                     AQIESSRYQREITDLEAVLYQEAPPSMTDFMSISAALHEMGFAIADREMGPGARDVAS
                     SVAMLAAQSEVGEAGYALPDIAQLRHDWEALRSEHFSDEEWFRESTPAVESAQEVPAP
                     TIDPSMIEDLLRAIDAIEALSDEGRRACEELGEPSYDFEQPGAEGEAHIEKWNDFARD
                     WDEDVTRVATRLPSPPSWDADPELTAAYQDVTFAIRELRNATMGPGSWPVPFEGDWTA
                     RFDEAERLLSQARERLAQ"
     gene            complement(6649..7050)
                     /locus_tag="VEC56_08190"
     CDS             complement(6649..7050)
                     /locus_tag="VEC56_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HXV14172.1"
                     /translation="MIKNVMFLLIGIVFGVTLYKAEIISWYRIQEMFRFHAFHMYGVL
                     GSAVAVAMISVAILSRSARREGAAIARRPFQHGVWIGGLIFGMGWALTGACPGPLWVH
                     VGAGTEVMLIAIASALVGVLAYSFIDPKLPH"
     gene            complement(7061..7618)
                     /locus_tag="VEC56_08195"
     CDS             complement(7061..7618)
                     /locus_tag="VEC56_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HXV14173.1"
                     /translation="MNEIASPAWPWYVAGPIVGLMVPLALLIGNRRWGVSSSLRHLCA
                     MASPIKPAFLRYNWRSEAWSLFFVAGAVMGGFIGAHVIPNAPVPDVAPRTVAAIQSLG
                     FESPRGLVPAEAFSFSQLATVRGFMMIVVGGFLVGFGTRYANGCTSGHSITGLALREL
                     PSLVATIAFFIGGLIMTHVVFEWIF"
     gene            complement(7602..8792)
                     /locus_tag="VEC56_08200"
     CDS             complement(7602..8792)
                     /locus_tag="VEC56_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXV14174.1"
                     /translation="MTHSTRSGILVALATIAFLVLAAEIVLRVAAPVADPTRSGAQHV
                     NATNPYIRFEYPRGYAAVTEVEPGLPGLEGRHFFTTNQYGFRGDSLAVPKPAREYRVF
                     VVGGSTAECFYLDDADDMSRVAQNELAALAGISRVVKVYNVGLSGTASDDHVAMIAQR
                     LVHLEPDAIVLLAGVNDLRRSIQGFDYLHYASHAPPPVPLYKRVLLSSQIVRRLAYFK
                     RRLDPEPADVLEARTLESDYARKIALQNATPGTDADVLADTTSYRTNLRSIAGIARAH
                     DFDLVFMTHPSSWNSEVDPGVRDFQWMRVYDGVVYREDTMDAALERLNDVMRAVSVED
                     SIPLCDLARVLPKSREYFYDDCHFNREGARAAGRELAALLATRVLAASAADVSKEDSG
                     ERNR"
BASE COUNT         1725 a         2750 c         2823 g         1569 t
ORIGIN      
        1 gctcaagcgg gacatcccca aggaccgggc gatccgggtc gacgatgtcg ttttcccgtc
       61 tggacgattg tgcgataggc ttcgccagga acaagacaag cacttcgctg taaaccggtg
      121 aggctggcgc caccccggcg cagaaggagg ggccgcacgt gcatctacat cgatcaatcg
      181 cattagttgc gaccgtcgcg gccctggcgt gtgggaaggg agagcaggct tcggagcccg
      241 cgaaagagca cgtcgttttc ggcgaattca tcgcgcccga ggactcgttt cctaagatcc
      301 gctactacga ggccggcctt gtctccccca acgaccgttg cgcggtgcgc aaggtccggc
      361 taaacgtgaa gatgccgccg gtctacgtca acggccggcc gatcggcttc tgctgatcgc
      421 cctgcgcagc gaccttcgtg caaggtcccg agccctatct gagcgacttc cacatccagc
      481 tcccctgcgc ggtggacacg accgcgttgg ccgtgctcga cccggcgcat cgcgccttcg
      541 tgaaccacga gatctatttc ttctccaccg acctggctcg cgagcagttc gaagccgagc
      601 cgtggcgcta cacggggaaa gtcaccgacc cggtaaccgt ggtccgcttc cggcccgacg
      661 cgtcatcgcc gtcgcgggcc gccgctggcc ggctgttcta cttcgagtcc gccgagacgg
      721 tcgcgcggtt cgatgaggac ccggcgcgct actcgacacc caagccaacg atgcacgagc
      781 tcgagtagcg ttggcttggc caggccttgt tccacgtgaa acggcgatgc acgtcgccgg
      841 tgctccacaa actccttgaa atcggggacg acccccggtt attcttcgtt tcggccgccc
      901 accagattcg gcttaagtta ttgtgatgaa aggtattagg atgcgattta aggtctgtgc
      961 gatgctggcc gcgctgggcg tgctcgcttc ttgtggaggc gacgaaggcg ggggcgtgga
     1021 gccggagaat cacccgccgg tagtcgccat gcaattttcc aaagtcggag ttgtaagaag
     1081 cactcccata cagccttccc ttgtgcaatt atccgtcaac gtcagcgatc ccgatagcga
     1141 ccccataaca atgacctggt cggtgacccg gggcaccatc acaccgctga acgccgcggg
     1201 tacgctggtc caatggaccg tcccgttgga agtcggcgtc gactcggtga ccgcctcggc
     1261 gaacgacggc acgacctctc ggagcgtcgt gaccgagatc aaggtcggta ccgcgttctc
     1321 cggggcgatc gctccggccc gcttcgaaca ggcgcgctcg ccttacatcg tcaccgtgac
     1381 gggtttctct ccggtgctgg cggtcaccgg tcgcgtgacc gtgatcgagc ccgggaccga
     1441 gttgcttttg gagaccgcca acaccgtcat cgatgtgacg gattccctca ttgccgtcgg
     1501 cacgccgggt gcgcccatcg tcattcgtcc caacgtgcgc aatctcacgt gcggcgacga
     1561 tcgcggatgg tgggaaggag tcaaggtgta ctccgatgct ccatctcacg gttacctcga
     1621 gttggattac gcggacttgt cgtatgggcg gttcagcgtg cgcctgcgcg atcagggttc
     1681 cgcggtgatt cgaaacagcg ccattcgctg cagcgggcag aacggcgtct tgcacgaagg
     1741 tggcggtgtg ctcctgctcg aggacacgca ggtgaccgac ggcgagttcg acggagtggc
     1801 cgtggattcg gacacgttcc aacccgattc gattctcgtg agcaactgcc ggatcaactt
     1861 caacgggcgc accggcgtga tgcttcgact caatgatccg ctgcaggaca cgcccatcct
     1921 gatcgagtac tcggaagtgc gattcaacgc cgagcacggg atcacgctcg agaacgcggt
     1981 gtttcccgag atccacttca acagtttctt cggcaatggt gcgggcagca cgagcggact
     2041 caacagtatt tggctcgtga acggctatcc cggaggcgca tcggtgaccg aagtcgacgc
     2101 gacgtgtaat ttctggggtg cgcccgtgac gaatcagtcc gtcatcgacg ccgtcgttcg
     2161 ggacaatacg gataccggag ccgtcggtgc gcgcgtcgtc acgagtccct ggtcgaacga
     2221 gaatccgctc gtgacgccat cgacgtgcgt ggtgccgtga ccccggccgc ttggcgggaa
     2281 gcaggaacat gggaaagatc atcgcaattg cgaatcaaaa aggcggcgtc ggcaagacga
     2341 cgacggccgt gaatctgtgc gcgagcctgg cggctgccga acgcaagacg ctccttgtcg
     2401 acatggatcc gcaggccaat gcggtctcgg gagtcggcat cgacaaggcg ctgatccagc
     2461 aaacgatcta cgaggttttg ttcgacgaca atctcgtact agacgcgatt ctcgaatcgc
     2521 cgatgccgtt tctacatgtg ttgccggcca aaccgtcgct agccggcgcc gaggtcgagt
     2581 tggtggcgga gccgcgacgc gaattccggt tgaagcgcgc gctcgcgcac atccgcgacg
     2641 agtacgacta catcgtggtc gactgtccgc cgagcttggg attcctgacc gtcaacacgc
     2701 tcaccgccgc cgactcgttg ctgattccct tgcagtgcga gtactacgcg ctcgagggac
     2761 tcacgcagtt gctatcgacg gtgaagatgg tgcaagcgtc gctcaatccg gacctcgcga
     2821 tcgaaggcgt cttactcacg atgtacgaca agcgcttgaa cctggccagc caggtcgcgc
     2881 aggaagcgat gaattatttc ggcgagaagg tctacaagac ggtgattccg cgcaacgtca
     2941 agctgagcga gtcgcccagt ttcggcaaac ccatcattct gtacgacgtt cagtgccaag
     3001 gcgcgaggag ctacctcgac ttggcgcgag aggtgatagg cgatgtccac taggaaagtt
     3061 ctcggtcgag gtctggaagc actcatcccc agcgccccca tgatcgagca ggcgcagccc
     3121 ggcgagcgcg tggtcgatgt ccccacggac gcagtcgacg cgaatccgtt ccagccgcgc
     3181 aagcgcttcg acgacgatcg cttgaaggaa ctcgcggaga gcatccgcac cgacggcgtg
     3241 ctgcagcccg tcgtcgttcg gcgcaagggc gccaagtacg agctcatcat gggcgaacgc
     3301 cgcctgcagg ccgcccgcct ggccggcgtg ccgaccattc ccgtggtcgt gcgcgacgtg
     3361 cgcgacgccg acgcgctgcg gctggccatc gtcgagaaca tccagcgcga gaacttgaac
     3421 gccatcgaag aggcgcacgc gttccgccgc ttgatctcgg agttcggctt gtcgcaggcg
     3481 gacgtcgcgg gactggtggg caaggaccgc agctccgtgg ccaatacgct tcgcttgctg
     3541 aacttgtcgg aggaagttca gcgcatggtc gaagatggca tcctcaccgg cggccacgcg
     3601 cgtgcgctgc tctcgcttcc cacccaaaaa gagcagctgg cgctcgcgcg ccgcatcgca
     3661 gagcagaact tgagcgtgcg ccaagtcgag gccgaagtgg gtctcacgcg cgtgaaaaaa
     3721 agcaacgcgg cgcggcgtca caaggagaag cccgcgtatc tcgcggatct cgagaaggcc
     3781 ttcgcgcaat acttggggac gcgcgtctcg atcgaagaga aacgcggggg caagggacgc
     3841 atccaggtcg agttctacag ctacgatgat ttcgagcggc tggcgggatt gatgcaaatc
     3901 ccgctccccc gctagcgcac tccatcgatg gcgaaagagt tctcggtcat catcgttccg
     3961 cacgaccacc ggaccacgtg gaatctgcgg gtctcctacc ggatggcgct cctgttcggc
     4021 atcctggccg gagtgtccgt gatctccggg atcgccttcg ttatcaccta tggaaagatc
     4081 gcgaccgcag cgaatcgctc cctgctcttg ggccgggaca acgagcgcat gcgcgccgag
     4141 ctcgcccaag tggactcgct caaggtggag ctgcttcgca tgcaggcgct cgggatccaa
     4201 ttcaaaggga tgctcggcgt ccccctgtcc cccgaggaca gtgttctagt ggccagcctc
     4261 tcgccggttg ccaagactcc agccatccca agtgacgagg aagagggagg ggttggaccg
     4321 ggtgagcagc gattgatgct cgactcaatg ccgtcgatgt ggccggtaaa aggctatgtc
     4381 acaagggaat tctcggtaac gggaggggaa aagagtcccg actatcaccc gggtatggat
     4441 attgctgccg cacgtaacac gccggtggtg gtggcagcgg atgggatcgt cgaggcgagt
     4501 gctttcgacg atgtctatgg gtggatggta gaggtcgatc acgggtacgg gattcggtcg
     4561 gtgtatggac ataactcccg taatctggta aacatcggtg accgggtgac ccgtggtaag
     4621 acgatcgcct tcgtagggag taccggcaag agcacggccc cgcatctgca tttcgaggtt
     4681 cgtgagaacg gcgttccagt cgacccgcgg aagtacctgt tgaattagct cgactgtagg
     4741 aacgctcaca atatgggggt ccggcatcat gttgaaatcg ggcatcaaga cgactcagtc
     4801 gaacgaggag gcgaaagtca tgtttggtaa agacgcggaa atcaccaccg ggcagtcgac
     4861 gttgaactcg atgctgggcc aagggtgtaa gatcaagggc gacatcgagc tgcagggtac
     4921 gattcgtatc gacggtcagt tcgaaggctc gatctcctgc ccggatacgc tcattatcgg
     4981 caagtcgggt gtggtcaagg cagacgtgaa ggtcaagaac gcggtcatcg gcggcaagct
     5041 ggtgggcaac atcagtgcgt cgaacaagat cgagctccag accggatcgc acgtcgaggg
     5101 cgacatcgcg acggcacggc tcgtgatcga cgaaggcgtg ttcttcgaag gcaactgcaa
     5161 gatgggtgcg accggaacgg gcacctcgca gtcccgcgac gtctcggatc acagcaagga
     5221 cagcaattgg gctcgcaagg acgagaagcg tccggagcca gtggctccca tcggggtcac
     5281 gcgctaaccc accggttcag cgcaaccaac catggaacgc cctcggggtt tcggccccga
     5341 gggcgtttct ttgttcgtga aacatatggg cacggcccag atttgccggc ggcgagaagt
     5401 tacgcgcacc ccgcttttcc acagtctggg aacaacctgt gcataagtcg agagctccct
     5461 cccgtggcgc ttactccatg taacgaaacg agttgaggcg gacggtcggt cgtcagaagg
     5521 gccgttttcc acaggcccga attgggaggg ctgctaacta cttgcgggac aatggagttt
     5581 ctttataggc ggcgcagtaa cgcttcaacc aggcgatctc atctgcttga ctcgccgcga
     5641 attagccggc cgccgggctg actattgcgc gagtcgctcg cgcgcttggg agagaagacg
     5701 ctccgcttcg tcgaagcgcg ccgtccagtc cccctcgaac ggaaccggcc agctccccgg
     5761 ccccatggtg gcgttgcgga gctctcgaat cgcgaacgtg acgtcttggt aggcagcggt
     5821 cagctcggga tccgcatccc acgacggcgg tgacggcaat cgagtggcca cccgggtgac
     5881 atcctcatcc caatcgcgcg cgaagtcgtt ccacttctcg atgtgcgctt cgccttcggc
     5941 gcccggctgc tcgaagtcgt aagagggctc gccgagctct tcgcacgcgc ggcgcccttc
     6001 gtcggatagt gcctcgatcg cgtcgatcgc gcgcagcaag tcctcgatca tcgagggatc
     6061 gatggtcggg gccggaactt cctgcgcgga ttcgaccgcg ggggtcgatt cgcggaacca
     6121 ctcctcgtcc gaaaagtgct ccgatcgcaa cgcctcccag tcgtgccgca gctgcgcgat
     6181 gtcggggagc gcgtagcccg cctccccgac ctccgattgc gccgcgagca tcgccacgct
     6241 gctggcgaca tcgcgcgcac ccggacccat ctcgcggtcc gcgatcgcga agcccatctc
     6301 gtggagggcc gccgagatcg acatgaaatc ggtcatcgac gggggcgctt cttgatacag
     6361 cacggcttcc aagtccgtga tctcgcgctg gtagcgcgag ctctcgatct gtgcgggcgt
     6421 ttcgtcgcga aggccctcgg ggtcgggagc ggcactcgtc ataccgcgcg tcgacagttt
     6481 catgacgccg gcgatagcga aggtaacgac gacggcggta atcatggtag cgggacgtgg
     6541 aagcatcgga attccctcca ggcagccggg gcgaaccaac cgccgcggct cgatctcgcc
     6601 ggatcgagca agagggattc caacccgggc cgcgggtata acccgctgct aatgcggcag
     6661 tttgggatcg atgaaggaat aggcgagcac ccccacgagc gcggaggcga tcgcaatcag
     6721 catgacttcc gtgccggcgc cgacgtgcac ccacagggga cccgggcagg cacccgtgag
     6781 cgcccagccc attccgaata tgaggccgcc gatccagacg ccgtgctgaa acggacgccg
     6841 agcgatcgcc gccccttcgc gtcgcgcgct gcgcgagaga atcgcgaccg agatcatcgc
     6901 caccgcgacc gcgctgccca ggaccccata catgtggaac gcgtggaacc gaaacatctc
     6961 ctggatccga taccacgaaa tgatctcggc cttatagaga gtcacgccga agacgatgcc
     7021 gatcagaagg aacatcacgt tcttgatcat gtagcaacct ctagaagatc cactcgaata
     7081 cgacatgggt catgatcaat ccgccgatga agaacgcgat cgtcgctacc aacgaaggca
     7141 gctcgcgcaa cgcgaggccg gtgatcgaat gtcccgacgt gcacccgttc gcgtagcgcg
     7201 tcccgaaccc caccaggaac ccgcccacga cgatcatcat gaaaccgcgc acggtcgcga
     7261 gttgcgaaaa cgagaaggcc tccgccggca cgagtccgcg cggcgactcg aatccgagcg
     7321 attgaatcgc cgcgaccgtt cttggcgcga cgtcagggac gggagcgttg ggaatgacgt
     7381 gcgcgccgat gaacccgccc atcaccgcgc ccgcgacgaa gaagagactc caggcttcgc
     7441 tcctccagtt gtagcgaagg aacgccggct tgatgggcga tgccatggcg cacaagtgtc
     7501 gcaacgacga cgagacaccc cagcgccggt ttccgatcaa gagagcgagt ggcaccatca
     7561 gtcccacgat cgggcccgcg acgtaccacg gccacgcggg actagcgatt tcgttcaccc
     7621 gaatcctcct ttgatacatc ggcggcgctc gctgcgagca cgcgcgtcgc cagcagcgcg
     7681 gccagctcgc gcccggccgc gcgcgcaccc tcgcgattga aatgacaatc atcgtagaaa
     7741 tactcgcgcg acttgggcag cacgcgcgcg aggtcgcata gcggaatcga atcctccacg
     7801 gagacggcgc gcatgacgtc gttcaagcgc tccagcgcgg cgtccatcgt gtcttcccgg
     7861 tacacgacgc cgtcatagac gcgcatccat tggaagtcgc gcacaccagg atccacctcc
     7921 gagttccagg agctggggtg cgtcatgaac accaggtcga agtcgtgcgc gcgcgcgatg
     7981 ccggcgatcg agcgcaaatt ggttcggtaa ctcgtcgtat cggcgagcac atcggcgtcc
     8041 gtccccggcg tggcgttctg cagcgcgatc ttgcgcgcgt aatcgctctc gagcgtgcgt
     8101 gcttccaaca cgtccgccgg ttcggggtcg aggcgccgtt tgaagtacgc gagtcgccgc
     8161 acgatctgcg agctcagcag cacgcgcttg taaagcggca ctggtggagg cgcatgcgac
     8221 gcgtaatgaa gatagtcgaa tccttggatc gagcggcgca agtcgttgac acccgcgagc
     8281 aaaacgatcg cgtcgggctc caggtgcacc agtcgctgcg cgatcatggc gacgtggtcg
     8341 tcgctggcgg tgccggagag accgacgttg tataccttga cgacgcgcga tatccccgcg
     8401 agcgcagcca gctcattctg tgccacgcgc gacatgtcgt cggcgtcgtc gagatagaaa
     8461 cactcggccg tgctcccacc caccacgaag acgcggtact cgcgcgccgg cttgggaacg
     8521 gcgagcgagt cgccgcggaa tccgtactga ttggtggtga agaaatgccg tccttcgagg
     8581 cccggcagtc cgggctcgac ctcggtcacg gctgcgtacc cgcgcggata ttcgaagcgt
     8641 atgtacgggt tggtcgcgtt tacgtgctgc gcacccgaac gcgtcgggtc ggcgacgggc
     8701 gcggcgacgc gtagcacgat ctcggccgcg agcacgagaa acgcgatggt cgcgagcgcg
     8761 actaggatac cggagcgcgt ggagtgggtc atggaaggtt cgcgctattc taccgcgcgg
     8821 cgcgattgac gcgcaaacgc cggcttcgta tgctgacgac ctcaggg
//
LOCUS       DASPGK010000298         7903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_661565, whole genome shotgun sequence.
ACCESSION   DASPGK010000298 DASPGK010000000
VERSION     DASPGK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661565"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VEC56_08205"
     CDS             complement(<1..320)
                     /locus_tag="VEC56_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV14175.1"
                     /translation="MQYEIQALNEKVERESRFVPVLQKSIQRVIVGQKYLVDRLLIGI
                     LCDGHVLIEGVPGLAKTLSVKTLAASVDAAFRRIQFTPDLLPADLVGTEVYNPRDHTF
                     TAKKG"
     gene            510..1403
                     /locus_tag="VEC56_08210"
     CDS             510..1403
                     /locus_tag="VEC56_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14176.1"
                     /translation="MITQEVAKSSRGKGRDAVAGARMPAYGWVGLASMIVFEVLLFAT
                     DSEFVGWFFTPVQWTGLILFLDGHLKRRRGTSLLADHFGEFVILCAVSIASWLVFEGY
                     NLLLKNWRYLSLPDNPWVRFPGYVWAFATISPGMFLIYETLSDMLPGEDRPTYPRLRN
                     AVFWPFVIFGAACLVVPLIWPSTHMTPLVWMGFAFLLDPINGRLGDRSILSEFFTGHF
                     RSMPIFFLAGLVAGLLWEFWNYWALAKWEYDVPYLGHVKLFEMPVLGFLGFMPFIIES
                     HAIYRFVRRLIPIRDTARYLG"
     gene            1405..2100
                     /locus_tag="VEC56_08215"
     CDS             1405..2100
                     /locus_tag="VEC56_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXV14177.1"
                     /translation="MTDDRLAHFARLFAANLVIFDFDGVLVDSERHHMASYNAVFQKY
                     GHTLDPVEYAKYWTSLGLGPKGEIERHRLNIDPDLIRREKRPAFSELCRNGTIRFFPE
                     ARELVDLLVRAKKTLAIASGTMKVDIEAILENEGLTEYFASVVGSDAAALKPDPESFE
                     MVLRDVGASPNEAVILEDAEKGVSAAVAARIPVVVVRTPETRTIDFAGADLILDSHAE
                     LIDLARQVWEART"
     gene            2101..2454
                     /locus_tag="VEC56_08220"
     CDS             2101..2454
                     /locus_tag="VEC56_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14178.1"
                     /translation="MGATYSTTAWSRSSIRGDTEAGLRLIRRLDESTDANADSEHWYL
                     VAGSYGLLGDHENSVRALRWAVERGFFNYTAMEIDPQFDSFRDDPEFQHVLEIAKQKH
                     DAFRKKHADKLPDRW"
     gene            complement(2532..2681)
                     /locus_tag="VEC56_08225"
     CDS             complement(2532..2681)
                     /locus_tag="VEC56_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXV14179.1"
                     /translation="MNVPAVGGWLFGTLGVSTGGGLLGAIAVATVGAVVLIFVVRLFK
                     KGKSA"
     gene            complement(2678..3964)
                     /locus_tag="VEC56_08230"
     CDS             complement(2678..3964)
                     /locus_tag="VEC56_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14180.1"
                     /translation="MSKQSRQRIVISLLFLLACVAGIVWYAESRRARPDEPRARPQAP
                     PPASLTFDGLVDSAETLGAQFRLALGAIPRHDEPRRLQSTLDYLQSVTYLYYDWASGR
                     GEEAISAEGIQRGYVMPAVESIARARPELAEAHPGLRSAGVVSLYFLDHKMLLTLGLV
                     LSTSGLGRFPSLELAPIAREVPMDQVVWGRRIDAAGFDLREPIILDIVSWSHGASTGA
                     NMRTAGTVVVHHLDREAEFVETLRGEIQHARHGPPDPPHDAPTLMRGLVYVALQPYLA
                     LDDEGAVRQFMKDWSEASYMHEAGHVFSRQAGLGLTPQTAQEEALAYLTELRFGGMPH
                     YTLRNMVSVGMVEGIRPHKDGMEVVFAEFSQHIFAATMRGDGFEAIDFRSIAESAAPG
                     AGFGAIAFLFPKLRADEIRALADRVFEEKYRHLVKE"
     gene            complement(3997..4197)
                     /locus_tag="VEC56_08235"
     CDS             complement(3997..4197)
                     /locus_tag="VEC56_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6116 family protein"
                     /protein_id="HXV14181.1"
                     /translation="MSIGIQSRVQEFASRLRFPRLFGITLAVLVVDLLVPDLIPFVDE
                     ILLALATALLGSWKRRRTPPPR"
     gene            complement(4292..4765)
                     /locus_tag="VEC56_08240"
     CDS             complement(4292..4765)
                     /locus_tag="VEC56_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HXV14182.1"
                     /translation="MSPAILALIICMVSAVLEGVMAGRGVRSRFRELRLPSYSPPMKV
                     WSVIGIAYYLICYVILYRLLGAGVSGQRHQAAFLLLMVLMVVNAGWGYLFFRRKDLRA
                     SFLVFVPYGALALVLAFLLAGIDRASALLLILYLGYLAYAAWWAYRVWRLNHDPE"
     gene            complement(4762..5481)
                     /locus_tag="VEC56_08245"
     CDS             complement(4762..5481)
                     /locus_tag="VEC56_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV14183.1"
                     /translation="MIPTIRCRRMAASLDFYTRVLDFERVEGGEDGDPSFCVLVREGE
                     RVFLSSHGGDGEFGQAIVVATDDVDALFRKFVARGLRTPGRPESPVHEGPLDQTWGTR
                     EFYVDDPDGNTLRFVQGLQFRPTVNKRTVEVYMDGFRRTDRSQILSCLSDDVEWEIPG
                     MFHTRGKDEFATHIVDEGFAEKPAITVTRMVEAGDVVVAEGSVRGKRTDGSAFGVVFC
                     DVFEMRDGKIRKLVSYLMEIK"
     gene            complement(5478..5957)
                     /locus_tag="VEC56_08250"
     CDS             complement(5478..5957)
                     /locus_tag="VEC56_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXV14184.1"
                     /translation="MSPKRAAKKSAKSTPATRKQSKGFTDDERAAMKERAQELKAEAR
                     ADRDRAAGEKDVLAKIAAMSEPDRTMAKRLHAIIAASAPALSPKTWYGMPAYAKEGKI
                     VCFFQSAQKFKTRYATLGFSDEAHLDEGVMWPTSFALKELTAAEEARIVALVKKACR"
     gene            complement(5981..6304)
                     /locus_tag="VEC56_08255"
     CDS             complement(5981..6304)
                     /locus_tag="VEC56_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14185.1"
                     /translation="MEYTNQQRAAFKDAYAKRFRKQLIMLVLVFAAMAALAITSERAT
                     LFGLPAAVAGPISFVVVVLCWWIFEIRNWCCPACDKYLGRAFNPKRCRSCGIELRRTK
                     SDSVR"
     gene            complement(6314..6739)
                     /locus_tag="VEC56_08260"
     CDS             complement(6314..6739)
                     /locus_tag="VEC56_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14186.1"
                     /translation="MGEDLAGANVNRSYALASTNIAIFSFMLFFLYPRFEKGTIDPLL
                     FQATLVAMGLATFSLVFATLHYYRCSLGGRISEAERELHARRGDRLWLVGCTVMFLAP
                     SLVLFLVGLRAVASVWLILWLAYVLFVIRYFPKVETPRG"
     gene            complement(6780..>7903)
                     /locus_tag="VEC56_08265"
     CDS             complement(6780..>7903)
                     /locus_tag="VEC56_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14187.1"
                     /translation="APNGAVLSTVAWDGTGNGVIVAEGVGVTAVQRGAPGRLFRCDLR
                     SGEYRPLGWLENFPATIGLLPDGRLVLSSLIVRQNLREVSSTARTLADGRSLTSGMSI
                     DRQPVYSPDGKWVMFSSNRGGTLDLWEVSVETGEMHRVTDDPADDWDPAYSPDGQTIV
                     WCSSRSGAFEIWAARRDGTAPYQVSRDSLDAENPSISPDGRWVYYSSSHPRKTGLWRV
                     PSGGGIGERILVGSTLIPDLSRDGRHMSVILGVGTSANRLGVFDLEEGRELPAFVPLQ
                     VLQGSVQTGRSRMAPDGSSVVYLRVSENGQPVLVRRPLSAWRSGSGTTETLFADATEA
                     VESFGFSPDGTRAVVSVVDWLSGLTIAEVPGIVPPKRDR"
BASE COUNT         1457 a         2659 c         2400 g         1387 t
ORIGIN      
        1 cccttcttgg cggtgaaggt atggtcgcgc gggttgtaga cctcggtgcc caccaagtcc
       61 gcgggcaaca ggtcgggcgt gaattgaatg cgcctgaacg ccgcgtcgac cgacgccgcg
      121 agggtcttca ccgagagcgt cttggccagg cccggcacgc cctcgatcaa cacgtggccg
      181 tcgcacagaa tgccgatcag aagacggtcg accaggtact tctgaccgac gatcacgcgc
      241 tggatcgact tctggagcac gggaacgaag cggctctcgc gctcgacctt ctcgttcaag
      301 gcttgaatct cgtactgcat cgttacctcc gccggggctc ttgcgccggc aatcgactcg
      361 attgaacgtc ctcagggtgc gccggttccg gagccgaagt ggccatattc cggcgggttg
      421 cgccgctcgg ccgtcgtgcg ctaccatcgg cgcttcgcaa aagacacggg gtcggggaag
      481 cggcgcggaa aggcgaaaca ggaaccgcca tgataaccca agaagtcgct aagtcaagcc
      541 gtggtaaggg ccgcgacgcg gtcgcggggg cccgcatgcc ggcctacggc tgggtggggc
      601 tcgcctcgat gatcgtgttc gaggtcctgc tctttgcgac cgacagcgag ttcgtcgggt
      661 ggttttttac gcccgtccag tggaccgggc tgatcctgtt cctcgacggc cacctcaagc
      721 gccggcgcgg cacttcgctc ctcgccgacc acttcggcga gttcgtgatc ctgtgcgccg
      781 tctcgatcgc gagctggctc gtcttcgagg gctacaacct cctgctcaag aactggcgct
      841 acctgagcct gcccgacaac ccgtgggttc gcttccccgg gtacgtctgg gccttcgcca
      901 ccatatcgcc cgggatgttc ctcatctacg aaaccctctc cgacatgttg cccggcgagg
      961 accggccgac ctacccgcgc ctgcgaaacg cggtcttttg gcccttcgtg atcttcggcg
     1021 ccgcgtgcct ggtggtcccg ttgatatggc cgtcgaccca catgaccccg ctggtctgga
     1081 tgggcttcgc gtttctcctg gaccctatca acggacgact gggcgaccgt tcgatcctct
     1141 cggagttctt caccgggcac ttccggtcga tgccgatctt ctttctcgcg ggactcgtgg
     1201 ccgggctgtt gtgggagttc tggaactact gggcgctcgc caagtgggag tacgacgtgc
     1261 cctacctcgg ccacgtgaag ctcttcgaga tgcccgtgct cggtttcctc ggcttcatgc
     1321 cgttcatcat cgagagccac gccatctatc ggtttgtacg acgactcatc cccatccgcg
     1381 acaccgcgcg ctacttgggt tgacgtgacc gacgaccgcc tcgctcattt cgcgcggctg
     1441 ttcgccgcga atcttgtcat cttcgacttc gacggcgtgc tcgtggacag cgaacgccat
     1501 cacatggcgt cgtacaacgc ggtgttccag aagtacggcc acaccctcga ccccgtcgag
     1561 tacgcgaagt actggacgtc gctgggcctg gggccgaagg gcgagatcga gcggcatcga
     1621 ctgaacatcg accccgacct tattcgtcgc gagaagcgcc ccgccttctc cgagctgtgc
     1681 cgcaacggga ccattcgctt cttccccgaa gcgcgcgagc tggtggattt gttggtacgc
     1741 gcgaagaaga ctctggcgat cgcgagcggc accatgaagg tggacatcga ggccattctc
     1801 gaaaacgaag gcctcaccga gtatttcgcg tccgtcgtcg gcagcgacgc cgccgctctg
     1861 aagccggacc cggaaagctt cgagatggtg ctgcgtgacg tcggcgcgtc accgaacgaa
     1921 gcggtcatct tggaagatgc cgagaagggt gtgagcgccg ctgtcgcggc gcgtatcccc
     1981 gtcgtcgtgg tgcgcactcc cgagacgcgc accatcgatt ttgccggcgc ggatctgatt
     2041 ctagactcgc acgcggagtt gattgacctg gcgcggcaag tgtgggaggc ccgaacgtga
     2101 ttgggcgcga catactccac taccgcgtgg tcgcgaagct cgatccgcgg cgacaccgag
     2161 gccgggttgc gcctcatccg ccggctggac gagtccaccg acgcgaacgc ggactccgag
     2221 cactggtacc tcgttgccgg ttcatacggt ctgctgggcg accacgagaa cagcgttcgc
     2281 gccctgcgct gggcggttga acgcggtttc ttcaactata ccgcgatgga gatcgacccc
     2341 cagttcgatt cgttccgcga cgatcccgag ttccagcacg tgttggagat cgcgaagcag
     2401 aaacacgacg ccttccgaaa gaagcacgcc gataaacttc ccgatcgctg gtaaatctct
     2461 taccgccgct gcggcggtaa cgttgttgcc agtgtcgacg gaccgacacg ctggatgggt
     2521 cgaccagacg actaggccga tttccctttc ttgaagagcc gcaccacgaa gatcaagacg
     2581 accgccccga ccgtggccac cgcaatcgcc cccagcaacc cacccccggt ggacacaccc
     2641 aacgttccaa agagccaccc acccactgct ggaacgttca ttctttcacg agatgacgat
     2701 acttctcctc gaaaacccga tccgccagcg cgcgaatctc gtccgcccgc agctttggaa
     2761 acaggaacgc gatcgcgccg aatccggcgc caggggcggc cgattcggcg attgagcgga
     2821 aatcgatggc ctcgaatccg tcgccgcgca tcgtggcagc gaaaatgtgc tgcgagaact
     2881 cggcaaagac gacctccatc ccgtccttgt gcggacggat gccctccacc atgccgacgc
     2941 tgaccatgtt gcgaagcgta tagtgcggca tgccgccaaa gcgcagctcg gtgagatacg
     3001 cgagcgcttc ctcctgcgca gtttgcggcg tgagcccaag acccgcctgc cggctgaaga
     3061 cgtgcccggc ttcgtgcatg tagctcgctt ccgaccagtc cttcatgaac tgcctcaccg
     3121 cgccctcgtc gtcgagtgct aggtacggct gcaacgcgac gtatacgagt cctcgcatca
     3181 acgtgggggc gtcgtgcgga ggatcgggcg ggccgtgacg cgcatgctgg atctcccccc
     3241 gcagggtctc gacgaattcc gcctctcgat ccaggtggtg gaccacgacc gtccccgccg
     3301 tgcgcatgtt cgcccccgtg gacgctccgt gagaccacga cacgatatcg agaattatcg
     3361 gctcgcgcag gtcgaagccc gcggcgtcga tccgccgccc ccaaaccacc tgatccatgg
     3421 ggacctccct cgcgatcggc gctagctcga gcgacggaaa ccgccccaag ccgctcgtcg
     3481 agaggacgag accgagagtc agtagcatct tgtgatcgag gaagtacaga ctcacaacgc
     3541 ccgcgctgcg caatcccggg tgagcctcgg ccagttcggg acgcgcccgt gcaatcgact
     3601 cgacggcggg catcacatac ccgcgctgaa tcccttccgc agagatcgct tcctcgccgc
     3661 ggccgctcgc ccagtcgtag tacaggtacg tgacgctctg gagatagtcg agcgtcgatt
     3721 gcaggcggcg gggttcgtcg tgacgcggta tcgcgcccag cgcgagtcgg aattgcgcgc
     3781 cgagtgtctc cgcgctgtcg accagcccgt cgaacgtgag cgacgccggc ggcggcgcct
     3841 ggggtcgcgc cctcggctca tccggacgag cgcggcgcga ctcggcgtac cagacgatcc
     3901 ccgccacgca cgcgagcagg aagagcaggg aaattacaat acgctgccgc gattgcttcg
     3961 acattgttac cgcgtgttct ctatcgacgc gtcgggctat cgaggcggag gcgtccttcg
     4021 tcgtttccac gagcccaaga gtgcggtcgc cagcgccagt agaatctcgt ccacgaacgg
     4081 gatgagatcc ggaaccagaa gatcgaccac caacaccgcg agcgtgattc cgaacagcct
     4141 cggaaaccgc aaccgcgacg cgaactcctg aactctggat tgaatgccga tactcatcgg
     4201 aacccccgct cgccgaccgg cagcagcggg catagatagc ggatggcaat agacacgttg
     4261 cgtctccttc cagcgtcttc cctacgcgga atcactccgg gtcatggttg agcctccaca
     4321 cacggtacgc ccaccacgcg gcgtacgcta gatatcccag gtacaaaatc aggaggagtg
     4381 cggatgcccg gtcgattccg gcgagcaaga acgcgagcac gagtgccagg gcgccgtacg
     4441 ggacgaatac caggaagctg gcgcgtagat ccttgcggcg gaagaagagg tagccccacc
     4501 cggcgttcac gaccatgagg accatgagga ggagaaacgc cgcctgatgc cgctggccgg
     4561 acacaccggc cccgagcagg cgatagagga tcacgtaaca gatgaggtag tacgcaatgc
     4621 cgatgacgga ccagaccttc atcggcggcg agtaggaggg cagccgtagc tcacggaatc
     4681 gactgcgaac ccctcgcccg gccatcaccc cttcgagaac cgccgacacc atgcagatga
     4741 tgagcgcgag aatcgcaggg ctcacttgat ctccatcaag tagctgacga gtttccgaat
     4801 cttcccgtcc cgcatctcga agacgtcgca gaagaccaca ccgaaagccg atccgtccgt
     4861 tcgcttaccc cgcaccgagc cctccgccac caccacgtcg cccgcttcca ccattcgcgt
     4921 gaccgtgatg gccggtttct ccgcgaagcc ctcgtcgacg atgtgcgtcg cgaactcgtc
     4981 cttgccgcgg gtgtgaaaca tccccgggat ctcccactcg acgtcgtcgg agaggcagga
     5041 gaggatttgc gaacgatcgg tcctccggaa gccatccatg tacacttcga ccgttctctt
     5101 gttgacggtc ggccgaaact gcaatccctg cacaaagcgc agggtattcc cgtccggatc
     5161 gtccacgtag aattcgcgcg ttccccacgt ctgatccagc gggccctcgt gcaccgggct
     5221 ctccgggcga cccggcgtgc gaaggccccg cgccacgaac ttgcgaaaga gcgcgtcgac
     5281 gtcgtcggtc gccaccacga tcgcttgccc gaactcgccg tctccgccgt ggctggacag
     5341 aaacacgcgt tccccctcgc gcacgagaac gcagaacgac gggtcgccgt cctcgccgcc
     5401 ttcgacgcgc tcgaagtcca gcacgcgcgt gtagaagtcg agcgaggcgg ccatgcgacg
     5461 acagcgaatg gtgggaatca ccggcacgct ttcttgacca gtgccacgat ccgcgcctct
     5521 tcggccgcgg tcagctcctt cagcgcgaaa gaggtcggcc acatcacacc ctcgtcgagg
     5581 tgcgcctcgt cgctgaagcc gagggttgca tacctggtct tgaacttctg cgcgctttga
     5641 aagaagcaga cgatcttgcc ctccttggcg tacgcgggca tcccgtacca cgtcttgggc
     5701 gagagagccg gcgcgctggc tgcgatgatc gcgtgcagcc gcttggccat cgtgcgatcc
     5761 ggttccgaca ttgcggcgat cttcgcgagc acgtctttct ctccggccgc ccggtctcga
     5821 tcggcgcgcg cctccgcctt gagttcttgg gcgcgctctt tcatcgcggc tcgttcgtcg
     5881 tcggtgaatc ccttggactg ctttcgggtc gcgggagtgc tcttggcgga ctttttcgcg
     5941 gctctctttg gactcatagc tccctcccac tgagcgcggg tcaacgtacc gagtcagatt
     6001 tcgttcgccg cagttcgatg ccgcaactgc ggcaccgctt ggggttgaat gcgcgaccga
     6061 ggtacttgtc gcaagcggga cagcaccagt ttcggatctc gaagatccac cagcagagga
     6121 cgacgacaac gaaagagatc gggccggcga ccgccgcggg gagcccgaag agtgtggcac
     6181 gttcgctcgt gatggcgagc gccgccatcg cggcaaacac gagcaccaac atgatgagct
     6241 gtttgcggaa ccgcttcgcg taggcgtctt tgaatgcggc tcgctgctgg ttggtgtact
     6301 ccacggtttc tcctcaaccc cgcggcgtct cgaccttagg gaagtaccgg atcacgaaca
     6361 gtacgtacgc cagccagaga atgagccaga ccgatgccac cgcacggagc cccaccaaga
     6421 acagcactag actcggcgcg agaaacatca ccgtgcaacc cacgagccac aagcggtcgc
     6481 ctcgtcgcgc gtgcagctcc cgctccgctt cacttatccg gccgcccagc gagcatcggt
     6541 agtaatggag cgtcgcgaac accagcgaga acgtcgctag acccatcgcg accagagtgg
     6601 cctgaaacag gagcggatcg atcgtgccct tctcgaacct cgggtacagg aagaacagca
     6661 tgaacgagaa gatcgcgatg ttcgtcgaag cgagtgcgta cgatcgattg acgttggcgc
     6721 cggcaagatc ttctcccatc gaatgcctcc atactggtgg tgtcattgat ctccacaagt
     6781 caccgatccc gcttcggcgg cacgatgccg gggacctccg cgatcgtgag cccggagagc
     6841 cagtcgacca cggacaccac ggcgcgcgtt ccgtccggcg agaacccgaa cgattccacc
     6901 gcttcggtgg catcggcgaa gagagtttcc gtcgtcccgc tccccgatcg ccacgcgctg
     6961 aggggacggc gcaccagaac cggctggccg ttctcgctca cgcgtaggta gaccaccgat
     7021 gaaccgtccg gagccatccg agaccgcccg gtctgcacgc tcccctgtaa gacctgcagc
     7081 ggcacgaacg cgggcaactc cctgccttcc tcgagatcga acacgcccag ccggttcgca
     7141 gaagtcccca caccgagaat caccgacatg tggcgcccgt cgcgggacag atcgggaatg
     7201 agcgtcgatc ccaccaggat gcgctcgccg attccgcccc cactgggaac gcgccacagt
     7261 cccgtcttcc ggggatgcga cgagctgtag tacacccagc gcccgtcggg cgaaatcgac
     7321 gggttctccg cgtccaggct atcccgtgat acttggtacg gagcggttcc atcgcggcgc
     7381 gcggcccaaa tctcgaaggc gccgctgcgg ctggaacacc agacgatggt ctggccgtcg
     7441 gggctgtacg cggggtccca atcgtccgcg gggtcgtcgg tgacacggtg catttctccc
     7501 gtttcgaccg acacttccca gagatccagc gtcccgccgc gattcgacga gaacatgacc
     7561 cacttcccgt ccggcgagta caccggctga cggtcgatcg acatgccgct cgtgagcgag
     7621 cgtccgtcgg cgagcgtgcg cgcggtgctc gacacctcgc ggagattctg tcgaacgatc
     7681 aggctcgaga gcaccaggcg cccgtccggc aagaggccga tggtggcggg gaaattctcg
     7741 agccagccca gcggtcggta ttctcccgag cgcaggtcgc atcgaaacag gcgccccgga
     7801 gcgccgcgct gcacggccgt cacacccacc ccttcggcca cgatcacgcc gttgccggta
     7861 ccgtcccagg cgaccgtcga caggaccgct ccattgggcg cgg
//
LOCUS       DASPGK010000299         8592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_427916, whole genome shotgun sequence.
ACCESSION   DASPGK010000299 DASPGK010000000
VERSION     DASPGK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427916"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VEC56_08270"
     CDS             <1..503
                     /locus_tag="VEC56_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXV14188.1"
                     /translation="PLHGGANEAVLWMLEEIGSKERVAPWFEEKMAKKEKVMGMGHRV
                     YKTKDPRATILQGLARELFVKFGSSPLYDIAVELEKIAIDKLGPRGIYPNVDFYAGIV
                     YNRLAIPTDIFTPIFATSRIAGWAAHWMEQMRHNRLFRPTQVYQGEHNVPYVPMAERK
                     ERVKTA"
     gene            complement(584..787)
                     /locus_tag="VEC56_08275"
     CDS             complement(584..787)
                     /locus_tag="VEC56_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14189.1"
                     /translation="MERCAYCGEKVGDDGLYVDDEIFCSDECMRDFGAGGAYDEEDDD
                     DYEDDDEDEEEEESDDKDDDDDY"
     gene            1253..2872
                     /gene="aceB"
                     /locus_tag="VEC56_08280"
     CDS             1253..2872
                     /gene="aceB"
                     /locus_tag="VEC56_08280"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HXV14190.1"
                     /translation="MAPSMRSLVNPSIAEEGIVVTRPVGGDQAEILSPDAVRFVADLA
                     RRFTARRNELLKLRAIRQKEIDRGHFPDFLPETRSIREGSWKVAPIPNDLMDRRVEIT
                     GPVDRKMVINALNSGASTYMADFEDSNSPTWDNCVQGQVNLRDAVRRDIEFTSPEGKL
                     YRLNEKTATLIVRPRGWHLDERHFRVDGVPIPAGLFDFGIYFFHNAHELVERGTGPYF
                     YLPKMESHLEARLWNDVFLRAQDTLGIPRGTIRATVLIETILASFEMDEILYELRDHS
                     AGLNCGRWDYIFSFIKRFRGHRAFVLPDRALVTMDRHFLKSYVDLLVRTCHARGIHAM
                     GGMAAQIPIKNDPIANDSAIERVRADKLREVRAGHDGTWVAHPALVAVARDVFDAHMK
                     TPNQIRIAREDVAVTAQDLLAVPEGEITEKGLRTNINVGVLYLESWLRGSGCVPLYNL
                     MEDAATAEISRAQVWQWIRHGARLADGRGVTRELVQGVLKQEMDSIRAAVGESAFAAG
                     RYETASKLFAEMMTHEDFVEFMPSFAYDFLD"
     gene            2929..4215
                     /gene="aceA"
                     /locus_tag="VEC56_08285"
     CDS             2929..4215
                     /gene="aceA"
                     /locus_tag="VEC56_08285"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXV14191.1"
                     /translation="MTRRTFIDKTTTAWRTSPRWRGIERPYSAEDVYRLRGSFHIEHT
                     IAKLGAERLWELLHSEDYVHSLSAVTGNQAIQHVRAGLKAIYVSGWQTAADANTASAV
                     YPDLSLYPCDSVPELVSRINNALLRADQIHHYKENKQNGHGTHWLAPIVADAEAGFGG
                     VLHAYEIMQHMIRAGAAGVHFEDQLASVKKCGHLGGKVIVPTQEFITKLVSARLAADV
                     LGVDAILVARTDAGSAGLLTSDVDPYDHEFLLGERTHEGFYRVKAGLEQAISRGLAYA
                     PYADLLWFETSHPDLDEAQAFADAIHERFPGKLLAYNCSPSFNWKANLDEKTIANFQA
                     LLGEMGYKYQFVTLSGFHSLNHAMFQLAQGYLTRGMAAYSELQQAEFAAEKSGYEAVR
                     HQEFAGTGYFDAINEIATGGMTSTLGMAGSTEEQQF"
     gene            complement(4294..4797)
                     /locus_tag="VEC56_08290"
     CDS             complement(4294..4797)
                     /locus_tag="VEC56_08290"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV14192.1"
                     /translation="MPIARTILAEFNHEMANTRKVLERVPEEHAAWRPHPKSYSLGDL
                     AMHLAGMPRWAVITCKQDELDFDPPGGKKDPKPVFQTRKTMLEMFDDNVFAAVSAIAD
                     ATDDDMKAPWRLLNRGVEVFKLPRVAVLRSFVMNHMIHHRGQMTVYLRLKNVPLPAIY
                     GNTADEK"
     gene            4899..6164
                     /locus_tag="VEC56_08295"
     CDS             4899..6164
                     /locus_tag="VEC56_08295"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HXV14193.1"
                     /translation="MGVLHDVFAAQLPDLRARRAEVLKQHGDRVISQVTAAQAFGGMR
                     DVRALVCDTSWVDPDRGLIIRDIPILHLLDRSPEDVFFLLLIGRLPAQDESRAVRAEI
                     AAQPRVPEIVWHAVEAMPRDSHPMSMLSAALLAMEHESVFAKRFDAGIARDEYWRPSL
                     DDALRLLGSLPTLAGGIYHIRYGKGARLAHDPAYDWAENYGHMLGVRDSLFAPAVRRA
                     AILQSDHEGGNVCAFACHVVGSVLSNPYLAVSAGFNGLAGPLHGRSAQESTQWVLGAI
                     DRYGGVPSTAQIEEYAWETLNAGRVVPGHGHAVLRGPDPRFTAMLDFGRQHFENDAVF
                     DTVSRMAEVVPAVLKKHGKAKNPYPNVDFGMGAVWHHFGLTELAYYTVPFAISLAMGM
                     LAQLVINRAVGSAIVRPRSVTTESIAKGE"
     gene            6166..6375
                     /locus_tag="VEC56_08300"
     CDS             6166..6375
                     /locus_tag="VEC56_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HXV14194.1"
                     /translation="MANHVYKQIVLTGTSKTSIEDAVNNAIAKAAETVRNLRWLEVTE
                     TRGHIENQKLSHWQVTITVGFTLED"
     gene            6378..7085
                     /locus_tag="VEC56_08305"
     CDS             6378..7085
                     /locus_tag="VEC56_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXV14195.1"
                     /translation="MARTIKQQARFAGLLYMVLGVTAPIGLLYVPGKLIVSGDATATA
                     DRIRASESLFRLGIAAELIPQVVGIFVALALYRLFKGVNKTLARQLLIFGSLVSVPIT
                     MLNVLNNVAALQVLSGAAYLSVFDQPQLDALAFLFIRLHSLGLDIASIFWGVWLFPFG
                     MLVIRSGFIPRILGYLMMVAGATYLANAFATLSLPQLVPLIEKVAMPLYFGEVPIMFW
                     LLIWGARGPLADTPVSE"
     gene            7273..8475
                     /locus_tag="VEC56_08310"
     CDS             7273..8475
                     /locus_tag="VEC56_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HXV14196.1"
                     /translation="MLLSLRSLSDSDLLFRVRDLTVRERTLTLLLLLHLNEVERRKLH
                     LKQGYSSMFDYCTSGLGYSEPAAVRRIRTARCIARIPEVYGLLESNQVNLSTIARVSR
                     VLTAGNKDALLERIRERTQREVDAIVAEYQPRAAIRDVIKPVVVRVPAATVSGALGAS
                     ASVAVPGAPGLAIESGALGRDASTDSKTSVNATVEAPADDCEKNAYRRCDGASHPPDC
                     VRSAETFVTEKRIQFQFAASETFQVKLEKVKSLAWHRLPPNPSLEHLFELALDALIER
                     HDPGARQERRQRHQEAAGATLPQRVKREAARAGRVGHRGSGTRYVATAMRDTVFARDG
                     SRCPFVGPNGKRCGSRSALQIDHIKPVARGGASTLDNLRLLCAYHNRLEAERLMGPLA
                     RSRPRLRE"
BASE COUNT         1497 a         2828 c         2713 g         1554 t
ORIGIN      
        1 gcccgctcca cggcggcgcc aacgaagcag tgctgtggat gctggaagag atcgggtcga
       61 aggaacgcgt ggctccctgg ttcgaagaga aaatggccaa gaaagaaaag gtcatgggca
      121 tggggcaccg cgtctacaag accaaggatc cccgtgccac catcctgcag ggcctcgcgc
      181 gcgaactgtt cgtgaagttc gggagctcac cgctctacga catcgcggtc gagctggaaa
      241 agatcgccat cgacaagctg ggaccgcgcg gtatctatcc caacgtcgac ttctacgcgg
      301 gtatcgtgta caaccggctc gccatcccga ccgacatctt cacgcccatc ttcgcgacct
      361 cgcgcatcgc gggttgggcc gcgcactgga tggagcagat gcggcacaac cgcctgttcc
      421 ggcccaccca ggtctatcag ggcgagcaca acgttcccta cgttcccatg gcggagcgca
      481 aggagcgcgt gaagaccgcg tagccggtcg tcgcacggct ataataaaag ccgcccggag
      541 agactccggg cggcttttcg tttcctggat ttcgtggtag cgatcagtag tcgtcgtcgt
      601 cgtccttgtc gtcgctttcc tcttcctctt cgtcctcgtc gtcatcctcg taatcgtcgt
      661 cgtcctcttc gtcgtacgcg ccgccggcac cgaagtcccg catgcattcg tccgagcaaa
      721 agatttcgtc gtcgacgtag agcccgtcgt ctcccacctt ctcgccacaa taagcacagc
      781 gttccatgcg caatcgtcct ctcctagaga atgggcccct gtttccggcc gcccctgggg
      841 tgtggcgcgg gccgaccgga ccgacggctt tgcttattaa agtcgccgaa ggtcggtgtc
      901 aacaggaaaa cgccgaaggg tcgaacaagg cgtccccacg cctcgaatcg agcccgagaa
      961 atccgtgacc ccgcgccgca atggcgccgg ggcgaacccc gcgagccccg ttggaacgcg
     1021 gggcaaatgg cccaggatgg acgcgcgacc gcgcgtcccc ggcgccgggc tgcgcgcccg
     1081 cgcgacgacg aaccatcggt cgaaccatct gtcactccat gggatacgcg acggggcaag
     1141 gacgatcgga tcgccgcctg gcggcggcgt atggggtggt atgcgcgttg caattccgcc
     1201 tccggctcaa tcgaccgttc gccgctccgg gtcgttcttt cggaaggctt ccatggcccc
     1261 ctccatgcgc tccctcgtca atccgtccat cgcggaagaa ggcatcgtcg tgacccgccc
     1321 ggtcggcggc gaccaggccg agattctctc ccccgatgcg gtccgcttcg tggccgacct
     1381 ggcgcgccgc tttaccgcgc gccgcaacga gctcttgaag ctgcgcgcga tccgtcaaaa
     1441 ggagatcgac cgcggccact tccccgactt cctgccggag acgcgctcga ttcgcgaggg
     1501 atcgtggaag gtcgccccca ttccgaacga cttgatggac cggcgcgtgg aaatcacggg
     1561 gccggtcgat cgcaagatgg tgatcaacgc gctcaactcg ggtgcgtcga cctacatggc
     1621 cgacttcgag gactcgaact cgcccacctg ggacaactgc gtgcagggac aagtcaacct
     1681 gcgcgacgcg gtgcgccgcg acatcgagtt cacgagcccc gaaggcaagc tttaccggct
     1741 gaacgagaag accgcgactt tgatagtgcg cccgcgcggc tggcacctcg acgagcgcca
     1801 cttccgcgtc gacggcgtcc ccattcccgc gggcctgttc gacttcggta tctacttctt
     1861 ccacaacgcg cacgagctcg tggagcgcgg caccggaccc tacttctacc tccccaagat
     1921 ggagagccat ctcgaagcgc gtctgtggaa cgacgtgttc ctgcgcgcgc aagacacgct
     1981 cgggatcccg cgcggaacca ttcgcgccac cgtgctgatc gagacaattc tcgcctcctt
     2041 cgagatggac gagatcctct acgaattgcg cgaccactcg gccggtctca attgcggccg
     2101 ctgggactac attttttcgt tcatcaagcg gttccgtggt caccgtgcgt tcgtcctccc
     2161 cgaccgcgcg ctcgtgacca tggaccgcca tttcttgaag tcgtacgtcg atctcctcgt
     2221 gcgcacctgc cacgcccggg gcatccacgc gatgggcggc atggcggcgc agattcccat
     2281 caagaacgac cccatcgcca acgactccgc gatcgagcgc gtccgcgccg acaagctgcg
     2341 cgaagtgcgc gcgggtcacg acggcacctg ggtcgcgcac ccggccttgg tggcggtcgc
     2401 gcgcgacgtc ttcgatgcgc acatgaaaac gccgaaccag attcgcatcg cgcgcgaaga
     2461 cgtcgccgtg accgcccagg acttgctcgc ggtccccgag ggagagatca ccgagaaggg
     2521 gctccgcacc aacatcaacg tgggcgtgtt gtacctggag tcgtggctgc gcggctcggg
     2581 ctgcgtgccg ctctacaacc tcatggagga cgccgccacc gcggagatct cgcgcgccca
     2641 ggtatggcag tggattcgcc acggcgcgcg tctcgccgac ggccgcggcg tcacccgcga
     2701 gctggtgcag ggcgtcctga agcaggagat ggactcgatc cgggcggccg tgggcgagag
     2761 cgccttcgcc gcgggacgct acgagaccgc gtccaagctg ttcgccgaga tgatgacgca
     2821 cgaagacttc gtcgagttca tgccgtcgtt cgcttacgac ttcctcgact gacatgagat
     2881 cctaaatccc gtaccaacgt gcaccgcgcc caccaaggag gacacccaat gaccaggcgc
     2941 acgttcatcg acaagacgac taccgcgtgg cggacctcgc cgcgctggcg cggcatcgag
     3001 cgcccgtaca gcgcggaaga cgtgtaccgt ctgcgcggct cgtttcacat cgagcacacg
     3061 atcgcgaagc tgggcgcgga gcgcctgtgg gagctcctgc attccgagga ctacgtgcat
     3121 tcgctgagcg cagtcaccgg caaccaggcc attcagcacg tgcgcgcggg gttgaaggcc
     3181 atctacgtca gcgggtggca aaccgcggcc gacgccaaca ccgcttccgc ggtgtacccg
     3241 gacctgtcgc tgtatccgtg cgacagcgtc cccgagctgg tatcgcgcat caacaacgcg
     3301 ttgctgcgcg cggatcagat ccatcactac aaagagaaca agcagaacgg ccacggcacc
     3361 cactggctgg cgcctatcgt cgcggacgcc gaggccggct tcggcggcgt gttgcacgcc
     3421 tacgagatca tgcagcacat gatccgcgcg ggagccgcgg gcgtccactt cgaggaccag
     3481 ctcgcgtcgg tcaagaagtg cgggcatttg ggcggcaagg tcatcgtgcc cacgcaggag
     3541 ttcatcacca agctggtgag cgcgcgtctg gccgccgacg tgctgggcgt cgacgccatc
     3601 ctggtcgcgc gcaccgacgc gggcagcgcg gggctgctca ccagcgacgt cgacccctac
     3661 gaccacgagt tcctgctcgg cgaacggacg cacgagggct tctaccgcgt caaagccggt
     3721 ttggagcagg ccatctcgcg cggtctcgcg tacgcgccct acgcggacct cttgtggttc
     3781 gaaacctcgc acccggacct ggacgaggcg caggccttcg ccgacgccat ccacgagcgc
     3841 ttccccggga agctgctggc gtacaactgc tcgccgtcgt tcaactggaa ggccaacctc
     3901 gacgagaaga cgatcgcgaa cttccaggcc ttgctcgggg agatgggcta caagtaccag
     3961 ttcgtgacgc tctcgggatt ccactcgctc aaccacgcca tgttccagct cgcgcagggt
     4021 tacctgacgc gcgggatggc ggcgtactcg gagttgcagc aggcggaatt cgcggccgag
     4081 aagtccggtt acgaagcggt gcgccaccag gagttcgcgg gcacgggata cttcgacgcg
     4141 atcaacgaga tcgcgacggg tggcatgacg tcgacgctcg gcatggcggg ctcgaccgag
     4201 gaacagcagt tctagtcggc gccttcaggg tgcggggtcg gacgacgtgc ttttcctcct
     4261 tcgacgtcgt tcggccccgc ggtaccctct ctcctacttc tcgtccgccg tgttgccgta
     4321 gatggccggc agcgggacgt tcttcaagcg caggtaaacc gtcatctggc cgcggtggtg
     4381 gatcatgtga ttcatcacga aggatcgcag caccgcgacc cgcggtagct tgaacacttc
     4441 gacgccgcga ttcaagagtc tccacggcgc cttcatgtcg tcgtcggtgg catccgcgat
     4501 ggcggacacg gcggcgaaca cgttgtcgtc gaacatttcg agcatggtct tgcgcgtctg
     4561 gaagacgggc ttgggatcct tcttgccccc gggcggatcg aagtcgagct cgtcttgctt
     4621 gcacgtgata accgcccacc tcggcatgcc ggcgaggtgc atcgccaaat cgccgagcga
     4681 gtaggacttc gggtgcggac gccacgccgc gtgctcctcg gggacgcgct ccaacacctt
     4741 ccgcgtgttg gccatctcgt ggttgaactc ggccaggatc gttctagcga tgggcattcg
     4801 attgcctcct tggaacatct tcatactacg aaaccgggtc cgcctgcgac gattttcggg
     4861 gtcgtccggc cccgcggcag cgtgctagca tcgcgcgcat gggtgtcctc cacgacgtct
     4921 tcgccgcgca gctgccggac ctccgcgccc ggcgcgcgga ggtcttgaaa cagcacggcg
     4981 accgcgtgat ctcccaggtc acggccgcgc aggccttcgg cggcatgcgc gacgtgcgcg
     5041 cgctggtgtg cgacacctcc tgggtagacc ccgacagggg tctgattatt cgcgacattc
     5101 cgattctgca cttgttggat cggagccccg aagacgtgtt ctttctactg ctgatcggcc
     5161 gactcccggc gcaggacgaa tcgcgcgccg ttcgtgccga aatcgcggcc cagccccgcg
     5221 tgcccgagat cgtgtggcac gcggtcgaag cgatgccgcg cgattcgcac cccatgtcga
     5281 tgctctcggc cgcgctcctc gcgatggaac acgaatccgt gttcgcgaag cgcttcgacg
     5341 cgggtattgc acgcgatgag tattggcgcc cgtctctgga cgacgcattg cgactgctgg
     5401 gctcattacc cactctggcc ggcggcatct atcacattcg atacggcaag ggcgcgcgcc
     5461 tggcccacga tccggcctac gactgggccg agaactacgg gcacatgctg ggcgtgcgcg
     5521 actctctatt tgcaccggcg gtccgccggg ccgcgattct ccaatccgac cacgaaggcg
     5581 gcaacgtgtg cgcgttcgcc tgccacgtgg tcggctccgt gctgtcgaat ccgtatctcg
     5641 cggtgagcgc cggcttcaac gggctggcgg ggccgctgca cgggcgctcg gcgcaagaga
     5701 gcacgcagtg ggtgttgggt gcgattgatc gctacggcgg cgtgccatcg acggcgcaga
     5761 tcgaggaata cgcctgggag accctgaacg ccgggcgtgt cgtgccgggg cacgggcacg
     5821 cggtcctgcg tggtcccgac ccgcgtttca ccgccatgct cgatttcggg cgccagcact
     5881 tcgagaacga cgccgtgttc gacaccgtgt cgcgcatggc ggaagtcgtc cccgcggtct
     5941 tgaagaagca cggcaaggcc aagaacccgt atcccaacgt cgacttcggg atgggcgcgg
     6001 tgtggcacca cttcggcctc accgagctcg cgtactacac ggtgccgttc gcgatctcgc
     6061 tggcgatggg aatgctagcg caattggtga tcaaccgtgc cgtcggctcg gcgattgtga
     6121 ggccgcgcag cgttacgacg gagtctattg cgaagggaga atgagatggc gaatcacgtt
     6181 tacaagcaga tcgttctaac gggcacctcc aagacgagca tcgaggacgc cgtgaacaac
     6241 gcgatcgcga aggcggccga gaccgtgcgg aacctgcgct ggctcgaggt cacggagacg
     6301 cgcgggcaca tcgagaatca gaagctgtcg cactggcagg tgacgatcac ggtcggattc
     6361 acgctggagg actagctgtg gcgaggacga tcaaacagca agcacgcttc gccgggctcc
     6421 tgtatatggt gctcggggtc accgccccca tcgggctctt gtatgtcccc ggcaagctca
     6481 tcgtgtcggg agacgcgacc gcgacggccg atcgcatccg cgcgtccgag tctctcttcc
     6541 gcctcggtat cgcggccgaa ttgatacccc aggtcgtcgg aatcttcgtc gctctcgcct
     6601 tgtaccgctt gttcaaaggc gtcaacaaga cgctcgcgcg acagctgctg atcttcggct
     6661 cgctggtctc ggtgccgatc accatgctca acgtgctgaa caacgtcgcc gcgttgcagg
     6721 ttcttagcgg cgccgcctac ctgtccgtgt tcgaccaacc ccaactggac gcgctggcgt
     6781 ttcttttcat acgtctgcat tccctgggcc tggacatcgc atcgatcttc tggggcgtat
     6841 ggctgtttcc gttcgggatg ctggtgattc gatcgggctt catccctcga atactcggct
     6901 atctcatgat ggtcgcgggt gctacctacc tcgccaacgc gttcgccacc ctgtcgctcc
     6961 cgcagcttgt gccgctgatc gagaaggtcg cgatgccgct gtacttcggc gaagtgccca
     7021 tcatgttctg gctcttgatc tggggtgcgc ggggaccgct ggcggatacg ccggtgtccg
     7081 agtaggcttg tcacacgttc gcgcggttgc gcgggacgca ctcatgtcgt gcgcggtgta
     7141 cgacggcgcc tacgagagag cttttctcgc agcgcgctgc aattctctgt tgcctggtta
     7201 gcgaacatca ctatactgct ctcatagaca gcgctcctcc cgcgctcgtt gcaacgcaat
     7261 ggaggtttgc gcatgttgct ctcacttcgc tccctttccg attccgatct tctgttccgc
     7321 gtccgcgatc taacggtgcg cgagcgtacg ctgacgctac tgcttctcct ccacttgaac
     7381 gaagtcgaac gacgaaagct gcacctgaag cagggctact cgtccatgtt cgactactgc
     7441 acgtcgggac tggggtactc cgaaccggcc gcggtccgca ggatccgcac ggcgcggtgc
     7501 atcgctcgca ttcccgaggt gtacgggctc ttggagtcga accaggtcaa cctcagcacg
     7561 atcgctcgcg tctcgcgcgt gctgaccgcg ggaaacaagg acgcattgct ggagcggatc
     7621 cgcgaacgga cacaacgaga agtcgacgcg atcgtggcgg aataccagcc gcgcgcagcg
     7681 attcgagatg taatcaagcc ggtggtggtg cgggttccgg cggcgactgt gtcgggggcc
     7741 ctgggtgcga gcgcatcggt tgcggtccca ggagcgcccg gccttgcgat cgagtcgggt
     7801 gcgctggggc gtgacgcctc gaccgatagc aaaacttccg tgaacgctac cgtcgaggcg
     7861 ccggcggacg attgtgaaaa gaatgcttac cgtcgctgcg acggtgcttc tcacccaccg
     7921 gattgcgtgc gatccgcgga gacgttcgtc accgagaagc gcatccagtt ccagttcgca
     7981 gcgagcgaga cgttccaggt gaagctcgag aaagtcaaat cgctcgcctg gcatcgactg
     8041 ccgccgaatc cgtctctgga acacctattc gagctggcgc tggacgctct tattgagaga
     8101 cacgaccccg gtgcgcgcca ggaacgtcgt caacggcacc aggaagccgc cggagcaacg
     8161 ctgccgcagc gcgtgaaacg agaagcggcg cgagctggcc gcgttggaca ccgtggcagc
     8221 gggacgcgat acgtcgcgac ggccatgcgc gacacggtat tcgcgcgcga tggcagccga
     8281 tgtccgttcg tgggcccgaa cggcaagcgc tgcgggtcgc ggagcgcgct gcaaatcgat
     8341 cacatcaaac cggtcgcgcg cggcggtgca agcaccttgg acaacttgag gctgctatgc
     8401 gcgtatcata atcgcctgga agcggaacga ttgatgggac cgttggctcg ttcgcggccg
     8461 cggttgcggg aatgagcccg ctgccgcgtc acgacaacgg tgacggcggc gaccgcgagt
     8521 ctacacctcg accgaagcac ccgcaccgac cactttcgcg ctcatgccct tcgcttccac
     8581 cttgcgcacg aa
//
LOCUS       DASPGK010000300         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_778957, whole genome shotgun sequence.
ACCESSION   DASPGK010000300 DASPGK010000000
VERSION     DASPGK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778957"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..164
                     /locus_tag="VEC56_08315"
     CDS             6..164
                     /locus_tag="VEC56_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXV14197.1"
                     /translation="MNQADLTTMGDLLASGTIASVIDRRYPLSETADAVRYLEEGHAK
                     GKIVITMG"
     gene            195..461
                     /locus_tag="VEC56_08320"
     CDS             195..461
                     /locus_tag="VEC56_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14198.1"
                     /translation="MQVFLRHVAIPALAPAAIVALYFTPVMVFGCVNRGLLAFGVVLI
                     SSGAAFVTIGLGMRESLRRRSATWWLLSTLILVLPLVLILGPLG"
     gene            488..877
                     /locus_tag="VEC56_08325"
     CDS             488..877
                     /locus_tag="VEC56_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14199.1"
                     /translation="MSAETYTYHPPRWVRIAVAVSFLIFVAMLAIALVIDLKPMYVAL
                     SAGMVVFAALAVVESIVARVFVEPDAVVIKGLFKTERVALSNVEKVSAAGGGIALFLN
                     TKKWKKLPDWLGANMSARRRIADRIGH"
     gene            969..1442
                     /locus_tag="VEC56_08330"
     CDS             969..1442
                     /locus_tag="VEC56_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14200.1"
                     /translation="MPRLRLASLGTIAVAVTLLSSCDDAQISFATGAFVDAPAPQIRV
                     RWGPDFKMAPVILDSQYQRIGPFETSRSGELAIEFVVLAEGQESTTQGSFSLPLKSDW
                     SWSVDFHVADRDPAQGCFGCVGSRSYALDPTLGFAPEYELWVTWGGNSISNPVTY"
     gene            1459..1803
                     /locus_tag="VEC56_08335"
     CDS             1459..1803
                     /locus_tag="VEC56_08335"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXV14201.1"
                     /translation="MSAYLIARVDVQDWDRYREYMRHTPRVIVNFGGRFIVRGGETIT
                     VEGPQETRRVVVIEFPSFQQAKAFYESGEYRRIKEFRDGAGAAQFVIVDGYSLDDWNK
                     VAAESATLRMPD"
     gene            1895..>3070
                     /locus_tag="VEC56_08340"
     CDS             1895..>3070
                     /locus_tag="VEC56_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HXV14202.1"
                     /translation="MPSAQSSKSSVVFIFIAVLIDVIGFGIIIPVIPELIMELTGEGI
                     SRAAIYGGWLGFAYAVMQFLAASVLGNISDSVGRRPVILFSLAALGIDYIVMGLAPSL
                     PWLFLGRALAGIAGASFIPAYAYLADVSPPEKRAQNFGLVGTAFGIGFIIGPAIGGLL
                     GGFGTRTPFFVAAGLALANTAFGYFVLPESLPKERRRPFALKRANPVGALIQIRKYPV
                     VAWLAGSVFLWQVAHQVFPSTWSFYTMYRFGWTETLVGASLACIGAIMAVSQGILSRI
                     VVPSIGERRTALLGLACAICAHLGFAFATRSWMAFALLGAWLFAGLVYPSLNAIMSRE
                     VPPNAQGELQGAVATLFSIALVVSPPLMTQLFGRFSGPNAPIRFPGAAFACAALLTSA
                     "
BASE COUNT          453 a         1009 c          990 g          618 t
ORIGIN      
        1 cgagggtgaa ccaagcggat ttgactacca tgggggatct tctagcatct ggaacgatcg
       61 cgtccgtcat cgacaggcgc tatccgttga gcgagacggc cgacgcggtc cgctatctcg
      121 aagaagggca cgcgaagggg aagatcgtga tcacgatggg gtagcctggc gcgcgatttg
      181 ctccctccct ccgcatgcag gtgttcctcc gccacgtcgc catccccgcc ctcgcgccgg
      241 ccgcgatcgt cgccctctat ttcacgccgg tgatggtgtt cgggtgcgtg aaccgcggct
      301 tgctggcctt cggagtcgtg ctgatctcga gcggcgccgc gttcgtcacc atcggcctcg
      361 gcatgcggga atcattgaga cgcaggtccg cgacgtggtg gctcctctcc acactgatcc
      421 tcgtacttcc cctggtattg atcctgggcc ccctgggctg accgcaggat tctgctacac
      481 tccgagcgtg tcggctgaaa cctacaccta ccaccccccc agatgggttc gcatcgcggt
      541 cgcggtgtcg ttcctgatct tcgtcgccat gctcgccatt gcgcttgtga tcgatctcaa
      601 acccatgtac gtcgcacttt ccgccgggat ggtcgtcttc gccgccctcg cggtggtcga
      661 atcgatcgtc gcacgcgtct ttgtcgagcc ggacgcggtc gtcatcaagg ggctgttcaa
      721 gaccgagcgc gtggcgctct ccaatgtcga aaaggtctcg gccgcgggcg gtgggatcgc
      781 actctttctc aatacgaaga agtggaagaa gctcccggac tggctgggcg ccaacatgag
      841 cgcgcggcgg agaatcgcag accgtatcgg ccattgacgg gcgcgccgtt agccggcgaa
      901 gccattttgg cctcgcggcg tgggggcgca tgagctaaaa ttccaacgta accccgccct
      961 ctacgaccat gccacgattg cgcctcgcat cgttaggtac catcgctgtc gcggtgacgt
     1021 tgctgtcgtc gtgcgacgat gcccaaatca gcttcgcgac cggcgcattc gtcgacgctc
     1081 cggcgccgca gattcgtgtg cgatgggggc cggacttcaa gatggcgccg gtgatcctgg
     1141 attcccaata ccagcgcatc ggcccattcg aaaccagccg gtccggcgaa cttgcgatcg
     1201 aattcgtcgt cttggccgag ggccaagaat cgacgacgca gggttcgttc tcgctgccgt
     1261 tgaagtccga ctggtcgtgg agcgtcgatt tccacgtcgc ggaccgcgac cccgcgcagg
     1321 gttgcttcgg gtgcgtcgga tcgagatcct acgcgttgga cccgacgctc ggcttcgcac
     1381 ccgagtacga actttgggtg acgtggggcg gcaactcgat cagcaacccc gtcacgtatt
     1441 gaccccaagg agcccgccat gtccgcctat ctcatcgccc gtgtcgacgt ccaggattgg
     1501 gatcgctacc gcgagtacat gcgacacacg ccgcgcgtca tcgtgaactt cggcggccgc
     1561 ttcatcgtgc gcggcggcga gacgatcacg gtggaaggac cgcaggagac gcgccgcgtg
     1621 gtggtgatcg aattcccgtc gttccagcag gcgaaggcgt tttacgagtc gggcgaatac
     1681 cggcgcatca aggaattccg cgacggcgcg ggtgccgcac agttcgtcat cgtcgacggg
     1741 tactcactcg acgactggaa caaggtggcc gccgagagcg caacgctccg catgcccgac
     1801 tgacgtcgaa ccgctgccgc gccgttcacc cacgttgacg cgcgcgcgtt agaccgcgtg
     1861 acgcggcgcc cgtcgatact ctatactccc cgccatgccg tccgcccagt cctccaagtc
     1921 ctccgtcgtt ttcatcttca tcgcggtgct gatcgacgtg atcggtttcg gcatcatcat
     1981 cccggtcatc cccgagctca tcatggagct cacgggagag ggtattagcc gcgcggcgat
     2041 ctacgggggc tggctcgggt tcgcgtacgc ggtcatgcag tttctcgccg cctcggtact
     2101 cggcaacatc agcgactcgg tcgggcggcg gcccgtcatc ttgttctcgc tcgccgcgct
     2161 cggcatcgac tacatcgtca tggggctcgc gccgagtctg ccgtggctgt ttctcgggcg
     2221 cgcgctggcg ggaattgcgg gcgcgtcgtt cataccggct tatgcgtatc tcgccgacgt
     2281 cagtccgccg gagaagcgcg cccagaactt cgggctggtg ggcaccgcgt tcggtatcgg
     2341 cttcatcatc ggccccgcca tcggcgggct cctgggcgga ttcgggacgc gcacgccgtt
     2401 cttcgtcgcg gcgggtctcg cgctggccaa caccgccttc ggctacttcg tcctgccgga
     2461 gtcgctgccg aaggaacggc ggcgcccgtt tgcactcaag cgcgccaacc cggtgggcgc
     2521 gctgatccag attcgcaagt acccggtggt ggcgtggctg gccggctcgg tgttcttgtg
     2581 gcaggtcgcg catcaagtat ttccgagcac gtggtcgttc tacaccatgt accggttcgg
     2641 ctggaccgag accttggtgg gcgcgtcgct cgcgtgcatc ggcgccatca tggcggtgag
     2701 ccagggcatt ctctcgcgca tcgtggtgcc gagcatcggc gagcgccgca cggcgctcct
     2761 cggtctcgcg tgcgcgatct gcgcgcacct ggggttcgca ttcgcgacgc gcagctggat
     2821 ggcgttcgcg ctgttgggcg cgtggctctt cgcgggcctg gtgtatccgt cactgaacgc
     2881 gatcatgtcg cgcgaggtgc cgcccaacgc gcagggcgag ctgcagggtg cggtggcgac
     2941 cttgttcagc atcgcgctgg tcgtgagccc tcccttgatg acgcagcttt tcgggcgttt
     3001 ctcggggccg aacgccccca ttcgttttcc gggcgccgcg ttcgcgtgcg cggccctctt
     3061 gaccagcgcg
//
LOCUS       DASPGK010000301        12731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_779145, whole genome shotgun sequence.
ACCESSION   DASPGK010000301 DASPGK010000000
VERSION     DASPGK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779145"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VEC56_08345"
     CDS             <1..1364
                     /locus_tag="VEC56_08345"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXV14203.1"
                     /translation="VRADGSSIFADVAERVRPAVVFIKTERAMSASERGEGEPFEFFR
                     EFFPDQQQQPRGQRNRRVPGGGSGFVFDDEGHILTNYHVVRDAEKITVVLEKGEEEEE
                     HEARIVGADRHTDIAIIQLTDAPANMPKVPLGNSDAMRVGDWVMAIGTPFGQLQGTVT
                     VGIVSAKGRNDLNIVGGDATYQNYLQTDASINFGNSGGPLVNLKGEAIGINTAINPSG
                     QGIGFAIPINMAKSIMAELIARGRVRYGYLGIQLQELDKDLAQGMGLNIDQGIVVQGV
                     QQDTPAAKAGLQTGDVIIRFDGEDVRDEGKFRLKVAATPVGKTVPVVVVREGKEEKVS
                     VTLGERPDEDVVASVPTPDSDAWLGIHVADAQSAQVRRQYRLDRGEEGVVVMGVDEGS
                     PAEEAEIREGDVITEVFSHEVRSIDDYVTISKKLRERKDPIAFLVKRGRSTTFVTVIP
                     EGK"
     gene            1453..2319
                     /locus_tag="VEC56_08350"
     CDS             1453..2319
                     /locus_tag="VEC56_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV14204.1"
                     /translation="MDAELKKKEAGAGIRSLDLYLHEINRTPLLTREQERDLAKRIKG
                     GDKQALDQLVRANLRFVVSIAKQYANQGLSLEDLINEGNLGLIKAAHRFDEERGFKFI
                     SYAVWWIRQAMLQSLAEHSRIVRLPLNRAGTLYRIGKVSRQLDQELGRTPSADEIAAR
                     LKIPVQEVADTMVIANSHVSLDDPYSSEKDDTALVDYLTDETQASPDEDTYAAALSDD
                     LERALGTLSERERSILSMYFGLGGEEPMTLEDIGKRLKLTRERIRQIKEKAIQRLRHS
                     TRAKFLQGYMES"
     gene            2498..3415
                     /locus_tag="VEC56_08355"
     CDS             2498..3415
                     /locus_tag="VEC56_08355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV14205.1"
                     /translation="MIRDKILTFLYLPDGSHEPRAFQVRRNVVWALAGTVLVSLGASG
                     WAVLGHSSRMKDAYLVETLANQNRELRSEIDLLTGELETLKRQVAQNFDFQNKARIIA
                     NLEDLDRDVAEVGVGGPQMSFAQSLSSIDARTRDRLFTARGDIDKLLRQARLQTRDYE
                     EILSHLESANEQIRKTPSLRPVNVGFVSSRFGWRMDPISGRRAMHRGVDFSARMGTPV
                     YATADGVVTYSGVERTYGNVVEISHGSGFVTRFAHLQRRLVQKGQRVTRGDVVGRVGS
                     TGRSTFSHLHYEIEHNGERVDPLRFVLAD"
     gene            3526..4938
                     /locus_tag="VEC56_08360"
     CDS             3526..4938
                     /locus_tag="VEC56_08360"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXV14206.1"
                     /translation="MVSGAIAGSLAWPGPAVAERILRVDAIRHSVSEERTRVVIDLSR
                     PAEYSVTRLADSVGVSVSFRGAALSLDVVEIAVGRNGVRAVRPVVDAGVVSICFDLDR
                     PATWEVFALPAEGGKPNRVVVDLVPAPTAPAVATAPAPETTAPVSEPIADAVDRTAGD
                     TMADAGAPPPLATAPPMTKTAPSKPRPFVVAVDAGHGGHDAGAIGRYGLVEKKLCLDI
                     ARRVAEILNRNGNVRAVLTRDRDEYLTLPRRNELAEQKGADVFVSIHLNSAPSRSARG
                     AEIYFVAPAGAERAANQALASGEAAHDFGLEESGNSDLVHMLLDVNQQAVLARSELLA
                     ESILESVRKRALLPTRAVKQKSFSVLRTISMPCVLVEAGFISNNADAKLVKDPDGREK
                     VARAVADGVIEFFRAHPPQRVEVASTTASLIHRVQRGDTLWTLSRRYGTTVARIRELN
                     GLSPSQELHVGQELVVPSAN"
     gene            4964..5899
                     /locus_tag="VEC56_08365"
     CDS             4964..5899
                     /locus_tag="VEC56_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HXV14207.1"
                     /translation="MKPARAIANNFGAKVLAVVVALLVWFNASGQEVVSRVRTVPLLL
                     EGVSDSLALAGPVPVSAELRITATRRQLLALGFQRVAVVADLTGIEAGRRRVVLTGDH
                     VRGTDELDPSSIVVTQPSALEVVLEPLASRRLQVSLATTGALPANVMLEDGGLAIDPA
                     WVTVRGPASIIERIQHVTTETLELARVRETTTRELELECDPRAFGCDPDRVTVTLRVS
                     PRAERILANVPPTVLLDSDDMDAEVEPEAVSLTLEGPASVLDTLSSGDVSVLVSVAGR
                     EPASYRVVPDVILPLGVRLMEMSADTLEVRVFRTR"
     gene            6020..6487
                     /locus_tag="VEC56_08370"
     CDS             6020..6487
                     /locus_tag="VEC56_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXV14208.1"
                     /translation="MRLTELLVPEAVVTDFPPAPSGSKEDLLTAVLADLDQKGFVADR
                     RVATDDILARERVMSTGVGNGVAIPHAYTAGVDRLSAAFYRTREPVEFGGPDGAPVDL
                     FFVILGPRESRREHIRLLARLSRLLNHAEFREHLRAAADADAVIAVFRRFGDR"
     gene            6484..6903
                     /locus_tag="VEC56_08375"
     CDS             6484..6903
                     /locus_tag="VEC56_08375"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXV14209.1"
                     /translation="MSRPSFFSWSAAGVDRRWLAAAFAYAAVVFFVSSRPYLRPMGPE
                     FELKDNLAHAIEYGVLATLLFRALAPLTWPDAATTFLLIVAVAASVGAADEVFQGTVP
                     GRRRDIADWASDTTGAALAAAVCVWRARRTRATGMAP"
     gene            6900..8156
                     /gene="hisS"
                     /locus_tag="VEC56_08380"
     CDS             6900..8156
                     /gene="hisS"
                     /locus_tag="VEC56_08380"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXV14210.1"
                     /translation="MKYSRPRGTHDVTPTEVARWLAVEGVFQRALERYGYRGIRTPIF
                     ESTDLFVRAVGEQSDIVTKEMYTFNDRGGRSLTLRPENTASVIRAYLENGMHRWGGIQ
                     RLWYMGPMFRYDRPQAGRYRQFHQVGAEAIGSDNPALDAEIIQVVVDALTELGFTGVD
                     VRVNSVGTEASRARYRGVLLEAIEGAGSELAPDALARYRLNPLRIFDSKEYGTSLKRR
                     LPLLSDYLVDEDRSHFARVKELLASLSVPFTEDAHLVRGLDYYTRTVFEVYHGAHGAQ
                     SALCGGGRYDNLVAECGGPDTPAIGMSAGLERIVEALPAAALDDAGSAVVYYVACVGT
                     GAEARGLAVARVLREFGSAEVDVSGRSKKTQLEAAAKKKARVAVVVESDASDSLAWHD
                     LAQRVESRVAFSQLGAHARKGAGAKS"
     gene            8173..9966
                     /gene="aspS"
                     /locus_tag="VEC56_08385"
     CDS             8173..9966
                     /gene="aspS"
                     /locus_tag="VEC56_08385"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXV14211.1"
                     /translation="MSGSGYWLPFSRSHHLGELDASKVGATVRLMGWVARLRDLGGVR
                     FVELRDRYGRVQLVVDTTAEVGADTHSLRMEDVIAVEGEIRARPKDMVRDDAPAGAIE
                     VAVTRVVVLNKSDVIPFNIADDVKASEELRLKYRYLDLRRRPLKENIELRHKALLTIR
                     NFLNARGFLEIETPMLVRATPEGARDYLVPSRLHPGSFYALPQSPQLYKQILMVAGFD
                     RYFQIARCLRDEDLRADRQPEHTQIDIEMSFVEEKDVFALVEAMMADVFRATRNIALA
                     TPFRVMSYAQAMDRFGSDKPDLRFGMELRTLSESFAGTEFRVVRQALESGGAVRGFVV
                     DEGEKLAKRDVDELETAAKAAGAGGLLSLRCATGRLSGVAAKFLGESIEKRVIEGLGM
                     REGSLLLALVGKAPEISAILGRLRVDVGHRLGLDRRPGYEMLWINEFPLFERDPDTGG
                     YAPCHHLFSMPFEEDIQYLESDPLRVRAHLYDLVCNGTELASGSIRIHNRALQERVMA
                     VTGISREDAARRFGFLLNAFEFGAPPHGGIAPGVDRLIMTLVGAPSIRDVIAFPKTQK
                     AASLMDDAPSPVDPVLLRDLHIEIKKPKGHE"
     gene            10067..10417
                     /locus_tag="VEC56_08390"
     CDS             10067..10417
                     /locus_tag="VEC56_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14212.1"
                     /translation="MRKPIRAWLVGVAFAVCATTLGCGTAPTCNVSPVEIEELREDVA
                     VVNKNLVTAREREDNLTKELATKQADLDSKKDRPAELRARLEALRRGSGRLDKTKTTD
                     TKTAPKTGGAKERS"
     gene            10418..11098
                     /locus_tag="VEC56_08395"
     CDS             10418..11098
                     /locus_tag="VEC56_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV14213.1"
                     /translation="MERRAWVWVAVATVFMAGCGGSKVRKVDVSAGEYYSEEEFALLS
                     NSDKSAYCAALETERNRVQTEFTLAQEDLVATRRQIEGLRNQITPVEKEILRLESDIR
                     TLTGQIAALERLPREYTVKEGDCLSIIADRDDIYSAASKWPRIYRANLDKIEDPVWIY
                     PNMVLSVPRELPTEHRVAPFETLEMIAGYWEVYGDPAQWTRLFEANKDKVSDPTNLEP
                     NIVLTIPR"
     gene            11196..12227
                     /locus_tag="VEC56_08400"
     CDS             11196..12227
                     /locus_tag="VEC56_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXV14214.1"
                     /translation="MKTHRRVLSFSGVDPLKLFGPRDGHLAVVDRRYPGAVVVRGDDI
                     ILSGSHEQIERLTGFFREIIDIARRGRALSEQDINYILDKSNGGAGAAPVVAPSREYA
                     ALEDDVVVSTRRGAHIAPRTPGQKRYLDAIRSFDIVFGIGPAGTGKTFLAVAAAVAAL
                     RMDAVERIFLVRPVVEAGESLGFLPGDFQAKLDPYVRPVVDALSEMLGHDRTAKLMEN
                     GTIEIAPLAYMRGRTLNDAYVILDEAQNTTEMQMKMFLTRLGRNAKAIINGDVTQIDL
                     DPPARSGLLAAQRILSDVEGIAFVQLTQDDVVRHRLVQRIINAFDARDRQVADAKENV
                     PADSEESSR"
     gene            12224..>12731
                     /locus_tag="VEC56_08405"
     CDS             12224..>12731
                     /locus_tag="VEC56_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14215.1"
                     /translation="MIPRAVRNVLSFAKRPERGPRTPRSRRSAWRLPLALAFVAATVA
                     LLPPVRGAVDPVYREGHVADRDIAAPFALRVPLSGDDLRVARARASLAVPPVYRRERK
                     VERELTRDLSALLDSVATIVYARGLGDDERVARAAQWLPGVPRDVLRPALAPDGFGAL
                     HAAARDYQR"
BASE COUNT         2120 a         4205 c         4352 g         2054 t
ORIGIN      
        1 cggtccgcgc agacgggtcg tcgatcttcg cggacgtggc ggagagggtt cgtccggcag
       61 tcgtgttcat caagaccgag cgcgcgatga gcgcttccga gcgcggcgag ggcgagccgt
      121 tcgagttctt tcgcgagttc tttcccgatc agcagcagca gccgcgcggc cagcgcaacc
      181 ggcgcgttcc gggcggcgga tccgggttcg tattcgacga cgagggtcac atcctgacca
      241 actaccacgt cgtgcgcgac gccgagaaga tcacagtcgt tttggaaaag ggggaggaag
      301 aagaggagca cgaggcgcgc atcgtcggtg ccgatcggca cacggacatc gccatcattc
      361 aactgacgga tgcgcccgcg aatatgccca aagtgccgct tggcaactcc gacgcgatgc
      421 gcgtgggcga ctgggtgatg gccatcggta ctcccttcgg tcaactgcag ggaaccgtga
      481 ccgtgggcat cgttagcgcc aagggacgaa atgatctcaa catcgtgggc ggcgatgcga
      541 catatcagaa ctaccttcag accgacgcgt cgatcaactt cggcaacagc ggcggcccgt
      601 tggtcaatct gaagggcgaa gcgatcggca tcaacacggc catcaatccg tccggccagg
      661 gaatcggatt cgcgataccg atcaacatgg ccaagtcgat catggccgaa ctcatcgcgc
      721 gcggccgggt gcgttacggc tacctgggca tccagctcca ggagctcgac aaggatctcg
      781 cgcagggtat gggactgaat atcgaccagg gcatcgtggt acagggcgta caacaggaca
      841 cgcccgcggc caaggccggg ctccagaccg gcgacgtcat tattcgcttc gacggagagg
      901 atgtaaggga cgaaggcaag ttccgactca aggtcgcggc caccccggtg ggcaagacgg
      961 tcccggtcgt ggtcgttcgg gagggtaagg aagagaaggt cagcgtgacc ctgggcgagc
     1021 ggcccgacga agatgtggtc gcgtcggtcc cgacccccga cagcgatgcc tggctgggca
     1081 tccacgtcgc ggacgcgcaa agcgcgcagg ttcgccggca gtaccggctg gaccgtggag
     1141 aagaaggggt cgtggtaatg ggtgtggacg agggcagccc cgcggaggaa gccgagattc
     1201 gcgaagggga cgtgatcacc gaagtgttct cgcacgaggt gcgcagcatc gacgattacg
     1261 tcaccatttc gaagaagctg agagagagga aagatcccat cgctttcctg gtgaagagag
     1321 gaagaagcac gactttcgtg acggtgatac cggaaggaaa gtgagccctg gacggaactg
     1381 cgcccggggc gtcgagccag gagggaagca attatgatgg agcgcaagat ggacgataga
     1441 gaagaccgcg caatggacgc cgagctcaag aagaaggaag cgggggcggg cattcgcagt
     1501 ctcgatctct atctccacga gatcaatcgc acacccctgc tcacgcgcga acaggagcgc
     1561 gacctcgcga agcgcatcaa gggcggcgac aagcaggcgc tggaccagtt ggtccgagcc
     1621 aacctgcgct tcgtcgtctc gatcgccaag cagtacgcga accagggcct gtcgctggaa
     1681 gatctgatca acgaaggaaa cctcggactg atcaaggccg cgcaccgttt cgacgaggaa
     1741 cgcggattca agttcatctc gtacgcggtg tggtggattc gccaggcgat gctgcagtcg
     1801 ctcgcggagc actcgcgaat cgtgcggctg ccgctgaacc gggcggggac gctttatcgt
     1861 atcggcaagg tgtcgcgcca gctcgaccag gagctgggtc ggaccccgag cgccgacgaa
     1921 atcgcggccc gcttgaagat cccggtccag gaagtcgcgg acaccatggt gatcgccaac
     1981 tcgcacgtct cgctcgacga tccgtattcg agcgagaagg acgacaccgc gctggtcgat
     2041 tatttgaccg acgagacgca ggcgtcaccc gacgaggaca cctatgcggc ggccctttcg
     2101 gacgacctcg agcgcgcgct gggtacgctc tccgagcgcg agcggagcat cctgtcgatg
     2161 tacttcggcc tgggcggcga ggagccgatg accctcgagg acatcggcaa gcgcttgaag
     2221 ctcacgcgcg agcgcatccg ccagatcaag gagaaggcga tccagcggct gcggcattcg
     2281 acccgagcca agttcctaca gggctacatg gagagctagg cccgggcgcc ggaaggcatt
     2341 agcaccccag cggggccaga gatggccccg ctttttttgg tcgccgggcc ggccccacgc
     2401 ggtcgcgggc tggtacgaac cttgcgggcc gttgggtgga ttctcttgac accccgcggc
     2461 gggtccacta gcctcgtgcg tccgaaaggg aaaaccgttg attcgcgata agatactaac
     2521 gtttctctat cttcccgacg gcagccacga accgcgggcg ttccaggtcc ggcgcaacgt
     2581 cgtgtgggcc ctggctggca ccgtgctggt gtcgctgggc gcttcgggct gggcggtgct
     2641 cggtcactcg agccgcatga aggacgcgta tctcgtcgag acgctggcca accagaacag
     2701 ggagctgcgc tccgagatcg acctgttgac cggcgagctg gagacgctca agcgccaggt
     2761 cgcgcagaac ttcgacttcc agaacaaggc tcgcatcatc gcgaacctcg aggacctcga
     2821 tcgggacgtg gccgaagtcg gggtcggcgg gccgcagatg agcttcgcgc agtccttgtc
     2881 gagcatcgat gccaggaccc gcgaccgcct gttcacggcc cgtggcgaca tcgacaagct
     2941 cctccggcag gcgcggctcc agacgcgcga ctacgaggag attctctccc acctcgagtc
     3001 ggccaacgag cagattcgta agacgccgtc actacgcccg gtgaacgtgg gtttcgtgtc
     3061 gagccgcttc gggtggcgca tggacccgat ctcggggcgg cgcgcgatgc accgcggggt
     3121 ggatttctcc gcccgcatgg gcaccccggt gtacgcgacc gccgatggtg tggtgaccta
     3181 ctccggcgtc gaacgaactt acggcaacgt ggtcgagatc tcgcacggat ccggtttcgt
     3241 cacccgcttt gcccacctcc agcgtcggtt ggtccagaag ggccagcggg tcacccgcgg
     3301 cgacgtggtc gggcgggtgg ggtcaaccgg ccggtcgacg ttctcccacc tccactacga
     3361 gatcgagcac aacggcgagc gggtcgaccc gcttcgtttc gtcctggccg actagccccc
     3421 gcgttttccc ttgtcagggc tgtctccgaa ggctaatctt tcggggtcga ttgttattta
     3481 tctcgttata agataatggc ttatcgtaat tgctggaatt tgctcatggt ttccggtgcg
     3541 atcgccggga gcctcgcctg gccgggtccc gccgtcgccg agcggatcct ccgggtcgac
     3601 gccatccgtc actcggtctc cgaggagcgg acccgggtgg tgatcgatct ctcgcgaccg
     3661 gccgagtact cggtcacccg actcgcggac tcggtcgggg tctcggtgtc gttccggggg
     3721 gccgccctta gcctggacgt ggttgagatc gcggtgggcc gaaacggcgt gcgcgccgtg
     3781 cgcccggtcg tcgacgccgg tgtggtgagc atttgcttcg acctggatcg ccccgcgacc
     3841 tgggaggtct tcgcactccc cgccgaaggc gggaagccga accgcgtcgt cgtcgacctg
     3901 gttcccgcgc cgacggcccc ggcggtcgcg acggccccgg cccccgaaac cacggcgccc
     3961 gtttccgagc cgatcgcgga cgccgtcgac cggaccgccg gcgataccat ggccgacgca
     4021 ggcgcgccgc ctccgctcgc gacggcgccg cccatgacca aaaccgcgcc gtccaagccc
     4081 cgtccgttcg tggtagcggt cgacgcgggg cacggcggac acgacgccgg agcgatcggt
     4141 cgctacggac tcgtcgagaa gaagctctgc ctcgacatcg cgcgccgcgt ggccgagatc
     4201 ctcaatcgca acgggaacgt gcgcgcggtg ctgacgcgcg accgcgacga gtacctgaca
     4261 ctcccgcgcc gcaacgagct cgccgagcag aagggcgcgg acgtcttcgt cagcatccac
     4321 ttgaactcgg cgcccagccg ctcggcgcgc ggcgccgaga tctacttcgt ggcacccgcc
     4381 ggcgcggagc gcgcggccaa ccaggccctg gcgagcgggg aggcagcgca cgacttcgga
     4441 ctcgaggaga gcggcaactc ggacctcgtc cacatgctgc tcgacgtgaa ccagcaggcc
     4501 gtgctggcgc gcagcgagct gttggccgag tcgatcctgg aatcggtgcg caagcgcgct
     4561 ctcctgccca cgcgcgccgt caagcagaag tccttctccg tgctgcggac catttcgatg
     4621 ccgtgcgtgc tggtcgaagc cggcttcatc tccaacaacg ccgacgccaa gctggtcaag
     4681 gacccggatg gccgcgagaa agtcgcgcgg gccgtcgccg acggcgtgat cgagttcttc
     4741 cgcgcccacc cgccccagcg cgtcgaggtc gcgtccacga ccgcgtcgct cattcaccgc
     4801 gtgcagcggg gcgacacgct ctggacgctg tcgcgccggt acggaaccac cgtggcccgc
     4861 attcgcgagc tcaacggact ctcgccgtcg caagagttgc acgtcggtca agagctcgtc
     4921 gtgccgagcg cgaactgacc gcggcccgcc cgcgcgaagg ccgatgaaac ccgcccgcgc
     4981 catcgccaat aacttcggcg ccaaggttct ggcggtcgtc gtcgcgctct tggtttggtt
     5041 caacgcgagc gggcaagaag tggtctcgcg ggttcgaacc gtgccactac tcctggaagg
     5101 cgtttccgac tcgttggcgc tggcggggcc ggttcccgtc agcgctgaac tgcgcatcac
     5161 cgcgacgcgt cgccagttgc tggcattggg attccagcgc gtggcggtgg tcgcggactt
     5221 gaccgggatc gaagcgggac gccggcgcgt ggtgctcacg ggcgaccacg tgcgcgggac
     5281 cgacgagctc gatccgagta gcatcgtggt cacgcagccg tcggcgttgg aggtcgtcct
     5341 cgagccgctc gcgtcgcgcc ggctgcaagt ctcgctcgcg accacggggg cgcttcccgc
     5401 caacgtcatg ctcgaggacg gcggcctcgc gatcgacccg gcgtgggtga cggtgcgagg
     5461 gccggcgtcc atcatcgagc gcatccagca cgtgacgacg gagacgctcg agttggcgcg
     5521 cgtgcgcgag accaccacgc gcgagttgga gctcgagtgc gacccgcgcg cgttcgggtg
     5581 cgatcccgat cgcgtcaccg tgacgctgcg ggtgagtccg cgcgcggagc gaatcctggc
     5641 caatgtgccc ccgacggtgc tcctcgactc cgacgacatg gacgccgagg tcgagccgga
     5701 ggcggtttcg ctgacgctcg aaggccccgc gtcggtcctc gacacgcttt cgtccggcga
     5761 cgtctcggtg ttggtctcgg tcgcgggccg cgagcccgcc tcctaccggg tggtccccga
     5821 cgtgatcctc ccgctcggtg tgcgcctgat ggagatgagc gcggacacac tcgaggtgcg
     5881 cgtgttccga acgcgctaac gcgcggtcgt cgcgcggctt gacacgcggc cgatgccgca
     5941 cctaatgtgg atgctcgacc gtgtccatcc gatgccgcgc ccaaaggcgc gcagaacccg
     6001 tccgatcgtc caaacgtcca tgcgattgac cgagctactc gtcccggaag ccgtcgtcac
     6061 ggacttcccg cccgcgccga gcgggtccaa ggaggacctg ctcaccgccg tcctcgcgga
     6121 cctcgaccaa aaggggttcg tcgccgaccg gcgtgtcgcc accgatgaca ttctggcgcg
     6181 cgagcgcgtc atgagcaccg gagtgggcaa cggggtcgcg attccccacg cgtacaccgc
     6241 cggcgtcgac cggctctcgg ccgcgttcta tcgcacgcgg gaaccggtcg agttcggcgg
     6301 gcccgacggc gcccccgtcg acctcttctt cgtcatcctg ggtccgcgcg agagccggcg
     6361 cgagcacatc cgtttgctgg cgcggctatc gcggctgttg aatcatgccg agtttcgcga
     6421 gcacctgcgc gcggccgccg acgccgacgc ggtgatcgcg gtgttccgtc gcttcggaga
     6481 ccgttgagcc gcccgtcgtt tttttcgtgg tcggccgccg gcgtcgaccg ccggtggctg
     6541 gccgcggcgt tcgcctacgc ggcggtcgtg ttcttcgtct cctcgcgtcc gtacctgcgc
     6601 cccatgggcc cggagttcga gttgaaagac aacctcgcgc acgcgatcga gtacggcgtg
     6661 ctggcaacgc tgctgtttcg cgcgctggcg ccgctcacct ggcccgatgc cgcgaccacg
     6721 tttctgttga tcgtcgcggt ggccgcgagc gtgggtgctg ccgacgaggt gttccagggc
     6781 acggtgccgg gtcgccggcg cgacatcgcc gactgggcgt cggacacgac gggcgcggcc
     6841 ctcgcggccg ccgtgtgcgt ctggcgcgcg cggcgcacgc gtgcgacggg gatggcgcca
     6901 tgaagtattc gcgcccgcgc ggcacccacg acgtgacccc gaccgaggtc gcgcgctggc
     6961 tcgcggtcga aggcgtgttt cagcgcgccc tggagcgcta cggctaccgt ggcatccgca
     7021 cgcccatctt cgagtcgacc gacttgttcg tacgcgcggt gggcgagcaa tccgacatcg
     7081 tgaccaagga gatgtacacg ttcaacgatc gcggcggtcg cagcctcacg ctgcgccccg
     7141 aaaacacggc gtcggtgatt cgcgcgtact tggagaacgg gatgcaccga tggggcggca
     7201 tccagcgcct gtggtacatg gggccgatgt ttcgctacga ccgcccgcag gcggggcgct
     7261 accggcagtt ccaccaggtg ggcgcggaag cgattggcag cgacaacccc gcgctcgacg
     7321 ccgagatcat ccaagtggtg gtcgacgcgc tcacggagct cggctttacc ggtgtggacg
     7381 tgcgggtcaa ttcggtgggt acggaggcgt cgcgcgcgcg ctatcgcggc gtcctgctcg
     7441 aggcgatcga gggcgcggga agcgagctcg caccggacgc gctcgcgcgc taccgcttga
     7501 atccgctgcg catcttcgat tccaaggagt acgggacctc gctcaaacgg cggctgccgc
     7561 tgctctccga ttacttggtg gacgaggacc ggtcgcactt cgcgcgcgtg aaagagctgc
     7621 tggcgtcgct ctcggttccg tttaccgagg acgcccacct ggtgcggggt ctcgactact
     7681 acacgcgcac cgtattcgag gtgtaccacg gggcgcacgg cgcacagagc gcgttgtgcg
     7741 gcggcggccg ttacgacaat ctggtcgcgg agtgcggcgg gcccgacacg ccggcgatcg
     7801 gaatgagcgc ggggctcgag cgtatcgtcg aagcgctgcc cgccgcggcg ctcgacgacg
     7861 cgggatccgc cgtcgtttat tatgtcgcgt gcgtgggcac gggcgcggaa gcgcgcggcc
     7921 tcgcggtcgc gcgcgtcttg cgcgagttcg ggagcgccga ggtcgacgtg tcggggcgct
     7981 cgaagaagac ccagttggaa gccgcggcca agaagaaggc gcgcgtcgcg gtggtcgtcg
     8041 aatcggacgc gtccgactcg ctcgcgtggc acgacctcgc gcagcgcgtc gagagccgtg
     8101 tcgcgttttc ccagttgggc gcgcacgcgc gcaagggcgc cggcgccaaa tcctaatcca
     8161 ggagatcgaa tcgtgagcgg tagcggctac tggttgccat tctcgcgctc gcaccacttg
     8221 ggcgagctcg acgcgagcaa ggtgggcgcg acggtgcgcc tgatggggtg ggtcgcgcgg
     8281 ctgcgcgacc taggtggggt gcgcttcgtc gagctgcgcg accgctacgg gcgcgtccag
     8341 ctggtggtcg acaccaccgc ggaggtgggc gcggatacgc actcgctccg catggaggac
     8401 gtgatcgcgg tggagggaga gatccgcgcg cgtccgaaag acatggtgcg cgacgacgcg
     8461 cccgcgggtg ccatcgaagt tgcggtgacg cgcgtggtgg tgctcaacaa gtcggatgtg
     8521 attccgttca acatcgcgga cgacgtaaag gccagcgaag agctgcgcct gaagtatcgc
     8581 tacctcgacc tgcggcgccg tcccctcaag gagaacatcg agctgcgtca caaggcgctc
     8641 ttgacgatcc ggaactttct caacgcgcgc ggttttctcg agatcgaaac ccccatgctg
     8701 gtgcgcgcca ccccggaagg cgcgcgcgac tacctggtac cgagccgcct gcacccgggc
     8761 agcttctacg cgctcccgca gagcccgcag ctctacaagc agattctcat ggtggccggc
     8821 ttcgaccgct acttccagat cgcgcgctgc ctgcgcgacg aagacttgcg cgccgaccgg
     8881 cagccggagc acacgcagat cgacatcgag atgagcttcg tcgaggagaa ggacgtgttc
     8941 gcgctggtcg aggcgatgat ggcggacgtg ttccgcgcta cgcgcaacat cgccctcgcc
     9001 acgccgtttc gcgtgatgtc gtacgcgcag gccatggacc gcttcgggag cgacaagccc
     9061 gacctgcgct tcggcatgga gctgcgcacg ctctcggagt cgtttgcggg caccgagttc
     9121 cgagtggtgc gccaggcact ggagagtgga ggcgccgtgc gcggcttcgt cgtggacgaa
     9181 ggtgagaagc tcgccaagcg cgacgtcgac gaactggaaa ccgcggcgaa agccgcgggc
     9241 gcgggcggcc tgttgagcct gcgctgcgcg acggggcgac tctcgggcgt cgcggcgaag
     9301 ttcttgggcg agtcgatcga gaagcgcgtg atcgaggggc tcggcatgcg tgaagggagc
     9361 ttgttgctgg cgctggtggg gaaggcaccc gagatatccg ccatcctggg tcgcctgcgc
     9421 gtcgacgtcg gacaccgcct ggggctcgac cgcaggccgg gctacgagat gctctggatc
     9481 aacgaattcc cgttgttcga gcgcgacccc gataccggcg ggtacgcgcc gtgccaccac
     9541 ttgttctcga tgccgttcga agaggacatc cagtacctgg aaagcgaccc gttgcgagtg
     9601 cgcgcgcacc tctacgacct cgtgtgcaac ggcaccgagc tcgcgtcggg gagcattcgc
     9661 atccacaacc gcgccctcca ggaacgcgtg atggcggtca ccggcatcag ccgggaagac
     9721 gcggcccggc gtttcggctt cttgctgaac gcgttcgagt tcggagcacc cccccacggc
     9781 ggcatcgcgc cgggcgtgga ccggttgatc atgaccctgg tgggcgcgcc ctcgatccgg
     9841 gacgtgatcg cgtttccgaa gacgcagaaa gcggcctcgc tcatggacga cgcgccctcg
     9901 ccggtcgacc cggtcctgct tcgggacctg cacatcgaga tcaaaaagcc caaggggcac
     9961 gagtaggggc gggacgcttc tcccccttga cagccaccgc cttggctggt agcgtaccgg
    10021 cgttgtctcg ttctcctaac ccccacatat gaaaggagtc ttgggcgtgc gaaagcctat
    10081 tcgcgcgtgg ctagttgggg tcgcgtttgc ggtgtgcgca accacactcg ggtgcggtac
    10141 cgctcccacg tgcaacgtgt cgcccgtcga gatcgaggaa ctccgcgaag acgtcgcggt
    10201 ggtcaacaag aacctcgtga cggcgcgcga gcgcgaggac aacctgacca aggagctcgc
    10261 gaccaagcag gccgatctcg actccaagaa ggaccggccc gccgagctgc gtgcgcgcct
    10321 cgaagcgctt cgtcgcggca gcggccgtct cgacaagacc aagaccacgg acaccaagac
    10381 cgcgcccaag accggcggcg cgaaggagcg gtcgtaggtg gagcggcgcg cgtgggtgtg
    10441 ggtggccgtc gcgaccgtat tcatggcggg ctgcggcggc agcaaggttc gcaaggtcga
    10501 cgtttcggcc ggcgaatact actccgagga ggagttcgca ctcctgtcga actccgataa
    10561 gtccgcgtac tgcgcggccc tcgaaacgga gcgcaatcgc gtgcagaccg agttcacgct
    10621 ggcgcaggaa gacctggtgg cgacgcgccg tcagatcgaa gggctgcgca accagatcac
    10681 tccggtggag aaggagatcc tccgactcga atccgatatt cgaaccctga ccggacagat
    10741 cgcggcgctc gagcggcttc cgcgcgagta cacggtcaag gagggcgact gcctctcgat
    10801 catcgcggat cgcgacgaca tttactcggc ggcgagcaag tggccgcgca tctaccgcgc
    10861 caacctggac aagatcgaag atccggtgtg gatctatccc aacatggtcc tctccgtccc
    10921 ccgcgagctc ccgaccgagc accgcgtggc gccgttcgaa acgctcgaaa tgatcgccgg
    10981 gtactgggaa gtctacggcg accctgccca gtggacccgc ctgttcgagg cgaacaagga
    11041 taaggtcagc gatcccacca atttagagcc caacatcgtc ctcacgatcc cgcgctaact
    11101 cccttggcgg gagtcctccc ggtggcctat tattgcttac cggctaaggc gagcagactc
    11161 gccggccggc gtctcgcatt ggaggtgcga atcgtttgaa gacccaccgg agggtgctgt
    11221 ccttctcggg cgtcgaccct ctcaagctct tcggccctcg tgacggccac ctggccgtcg
    11281 tcgaccgccg ttaccccggc gccgtggtgg ttcgcggcga cgacatcatc ctgagcggca
    11341 gccacgaaca gatcgagcgg ctgaccggct tcttccggga gatcatcgac atcgcccgac
    11401 gcgggcgcgc gctttcggaa caggacatca actacatcct cgacaagagc aacggcggtg
    11461 ccggggcggc accggtggtg gcgccctcgc gcgagtacgc cgcactcgag gacgacgtgg
    11521 tcgtgtcgac ccgtcgcggg gcgcacatcg caccgcgcac gcccgggcag aagcgctacc
    11581 tggatgccat ccgctcgttc gacatcgtct tcggcatcgg gcccgccggc acgggcaaga
    11641 ccttcctcgc ggtggcggcc gcggtcgcgg cactccgcat ggacgccgtc gagcgcatct
    11701 tcctcgtgag accggtggtc gaagcgggcg agagcctcgg cttcttgccc ggtgatttcc
    11761 aggccaagct cgacccctac gtccgcccgg tggtcgatgc gctttcggag atgctcggac
    11821 acgaccgcac cgcgaagctg atggagaacg gcacgatcga gattgcgccg ctcgcgtaca
    11881 tgcgcgggcg cacgctcaac gacgcctacg tgatcctcga cgaagcgcag aacacgaccg
    11941 agatgcaaat gaagatgttc ctgacccgcc tgggccgcaa cgccaaggcc attattaatg
    12001 gtgatgtgac gcagatcgac ctcgatcctc cggcccgctc ggggctcctg gcggcacagc
    12061 gcatcttgag cgacgtggaa gggatcgcgt tcgtgcagct cacgcaggac gacgtcgttc
    12121 gtcatcgtct ggtgcagcgc attatcaacg ccttcgacgc gcgcgatcgc caagtcgcgg
    12181 atgcgaagga aaacgtaccc gccgattccg aagagtcgag tcgatgatcc cccgcgccgt
    12241 tcgcaacgtc ctgagcttcg cgaaacggcc ggagcgcggc ccgcgcaccc cgcggtcgcg
    12301 ccgctccgcg tggcgcctgc cgctcgctct ggccttcgtg gccgccacgg tggcgctcct
    12361 gccgccggtg cgcggcgccg tcgacccggt gtaccgggaa ggccacgtgg ccgaccgcga
    12421 cattgcggcc cccttcgcgc tgcgcgtccc gctctccggc gacgacctgc gcgtggcgcg
    12481 cgcgcgcgca tcgctcgcgg ttccgcccgt ctatcgccgc gagcgcaagg tcgagcgcga
    12541 gctcacgcgc gacctctcgg cgctgctcga ttcggtggcg accatcgtgt acgcgcgcgg
    12601 cttgggcgac gacgaacgcg tcgcgcgcgc ggcgcaatgg ctgcccggcg ttccccgcga
    12661 cgtcctgcgc ccggcgctcg cccccgacgg cttcggcgcc ttgcacgcgg cggcgcgcga
    12721 ctaccagcgc g
//
LOCUS       DASPGK010000302        17075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1130154, whole genome shotgun sequence.
ACCESSION   DASPGK010000302 DASPGK010000000
VERSION     DASPGK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 17075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17075
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130154"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VEC56_08410"
     CDS             <1..281
                     /locus_tag="VEC56_08410"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HXV14216.1"
                     /translation="HKLAGMIGALDGYSLRYRGPFFREFVVACPTDADAIIDAVRKHG
                     VLPGIPLSRYYGPVAHNLLLVTATDRHSDEDFELLCSALARASLVKVG"
     gene            285..1739
                     /gene="gcvPB"
                     /locus_tag="VEC56_08415"
     CDS             285..1739
                     /gene="gcvPB"
                     /locus_tag="VEC56_08415"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXV14217.1"
                     /translation="MDRKDFAQTIYGKSRRGRHGYHISSRATEAPRDIPREYLREGRI
                     GLPEVSEPQVMRHYVDLSIKNHHIDRDLFPLGSCTMKYNPKVNEDVAAMPGFAGLHPE
                     QGDDDAQGALAVMHALEQALIAVSGMDAVSAQPAAGAQGEMLGLLLARAYFADRGESR
                     HKVLVPDTAHGTNPATASVVGFETKTFASGKDGRVDLDVLRAAMDDDVAVLMLTVPNT
                     LGLFETRIADIVDIVHKKGGLCYMDGANMNALMGHARPGDFGFDMMHFNLHKTFSTPH
                     GGGGPGSGPVAVRSHLEPFLPTPRVVERDGGFRLEYQRPKSIGALHSFFGNFGIYLRA
                     YTYITRLGSDGIRRASSTAILNANYLAKRVSSRFPVPYGERCMHEFVATGEPLKKHGV
                     KTLDVAKRLLDFGFYAPTIYFPLVVPEALMVEPTETESKETLDRFAEALLQIAKEAVE
                     TPDVLTSAPTRTPVRRVDEATANRCPVITVPLDS"
     gene            1746..2720
                     /locus_tag="VEC56_08420"
     CDS             1746..2720
                     /locus_tag="VEC56_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14218.1"
                     /translation="MGSFLARRALQAVLLVAITLTLTFVLLHVAPGDPLARYLDPSVD
                     PADVERIRHSLGLDRPLHVRFIQWAKAFVVGDFGVSLAHHRPVRDLLAETIPRTLVLT
                     SVALAVQLVLGILVGTLAAHHRRRAVDRVLSFSVVLLYAVPSYYLAYLLIEVFAVDHS
                     WLPTSGISTPGLGATGAVLFWDHVRHLVLPAVVLGIASAAGFARFTRGSMVEVLAEDY
                     VRTARAKGLPESSVVWRHAFRNALGVLITLAGLSAPLLLGGAVIVETVFAWPGMGSLM
                     VESIYARDYPVVLAINFVGACMVIGGNFLADVATQRADPRATVEGGAP"
     gene            2810..3757
                     /locus_tag="VEC56_08425"
     CDS             2810..3757
                     /locus_tag="VEC56_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14219.1"
                     /translation="MVVVRFGEAVRLPVAEQGVVLAGWVLLLGGAAWAARATGRLRRR
                     FDADAVATIALCVLLSMAATSVLAPLVVGPDPADVAPATRYDAPSAGHPMGTDRFGRD
                     VWSRVVYGGRASFGVCALSVALAVAIGTLFGALSGMAPRRIDDAMMRTVDGLLSFPRL
                     LLLLTAVALVPPGAGALAVFIAATGWMGMARIVRGEVRRMRSREFVEAAVATGVGRSR
                     LLTRHLLPNALGALVVAATLNAGTVILIESSLSFLGLGLAPPTPSWGGMVFEGRDALS
                     TAWWVSAFPALAITAAVTALNLAGDGIRDALDARSSMEG"
     gene            3831..4850
                     /locus_tag="VEC56_08430"
     CDS             3831..4850
                     /locus_tag="VEC56_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14220.1"
                     /translation="MEAQGTDPRATALDAVDTLLDIRDLTVSFDTPDGRARAVDRVSF
                     TIRAGETLGLVGESGCGKTVSALALMRLIQTPPGRIEGGEILFKGTDLLKLPEKDMRK
                     IRGNEISMVFQEPMTSLNPVFTCGYQVEEAVVLHQKVSKREALERTLEMLALVKIPDP
                     KRVAKSYPHQLSGGMRQRVMIAMALSCNPSLLIADEPTTALDVTIQAQILELLQGLQQ
                     TFGMAILMITHDLGVIAETCRRVIVMYAGKVMEVASVNDLFHDPRHPYTRGLRESIPR
                     IAHKGERLKVIPGKVPDPLDLPKGCRFSDRCKYAEYRCDNEEVQLRDVKPGHSIRCWK
                     NIQDV"
     gene            4843..5865
                     /locus_tag="VEC56_08435"
     CDS             4843..5865
                     /locus_tag="VEC56_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14221.1"
                     /translation="MSSPQVEIQAGERTSARRGEVLLKVDNLCKHFPIRRGVLSRVVG
                     WIRAVDGVGFEVYRGETLGLVGESGCGKTTLARAILRLTDITSGTVTFDGIPVFEQNA
                     SDMRKLRRRMQIVFQDPYGSLNPRLTVGSMIAEAIKIHKLAAGEAVRERVASLLDRVG
                     IPAEYMNRYPHEFSGGQRQRIGIARALAVEPELIIADEPVSALDVSIQAQIINLLKDL
                     QDALGLTYLFIAHDLGVVEYISDRVAVMYLGRIVELAPASKLYRDPKHPYTQALLSAV
                     PSLEPGVKRQRILLPGDVPSPADVPRGCAFHTRCPHAMSICREQTPPQREVEPGHRTA
                     CWLYEK"
     gene            6301..9399
                     /locus_tag="VEC56_08440"
     CDS             6301..9399
                     /locus_tag="VEC56_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV14222.1"
                     /translation="MFPRDSRGAMIVAIVVALLGLTSSVAMAQTGTVTGTVVSAKDNK
                     PLAYANVMIVGTTMGAMSLSDGKFEIKGVPIGTYTVKAMMMGYSAVERAGVIVNAGAA
                     ANVAFKLDEKVVAQIQEIVVTADKAMVEVTSTEVRSGVTSEQVLDMPVDDVVDAIGLK
                     AGIVKTGDDMHVRGGRSNEVQIQIDGVPVSDPLGGGSVDVGMLGTSGSEIVSGGMDAE
                     YGDAQSAVINIATKEGGATFGGEVRYFTDDFGRSDKTYTNFDRLSLGFGGPTWWKNLR
                     YYLSGEATWSDTENTTIEPREEHKITDWFKFRQRQTSAYNVQGKLTYKMARMKVDGEM
                     IYSQLREDDYINNWNIEGYASKVWYFQGLVVAGPPVNGREQFQFDRLRSINHGPWADT
                     KDPAKQQAQLNVRPVDVIDLVRDPNTGQQNVVTYTDFRAADFRVGNQTITVVWDEAVQ
                     GPGGALSYKPWALFEGFQRGASDFKPFLTAPQGADSAFVPFNSAMHTPETRSENLQLK
                     LGFSHNITSKLLYTLKLSRLDLRSKSSVLDEFGDQKDPAQFSTAGLPVTLPDGTYLNA
                     GVSNAVWYTDDDFPYFVTAYDYPFYSDQHSLQYLFKGDVVSDQFKGHRIKTGVQVLYN
                     DLNDDERVNPAQTRIDEYGRIQQGLNVNTYENFNAEGAVYVQDKWDYEGMVVNGGLRY
                     EWFTVGNNSQVQIASSEIDQSVDEFKWNLSPRLGVAFPITDRDKFFFHYGRFTQWPSR
                     VYLFRTQDPVGSTGTLGNPNLEPELTVSYQAGISHQFTDDIAANFVVFNKDIYGLISS
                     TRVTDDSTGVQAFRFINRTYASSRGVELSLEKRLTRRLGFEVYYTYSFADGVASDSDF
                     GRSAEGLTHLPTEEYPLDWDQRNTFNVTLRLQDRNKWGATMIYQYGSGLPWTPVDRFA
                     RLNDPTWENSRRLEPTHKLSIQGRKLFNVYGRELTLFFEGRNLLDEDVLLPGGDAPNA
                     FPGLVAAQMDGGSYLTETGEYGGAYLQDQDADGRDDFNPVFDPTIWETHRLWRLGLGF
                     EF"
     gene            9436..10383
                     /locus_tag="VEC56_08445"
     CDS             9436..10383
                     /locus_tag="VEC56_08445"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HXV14223.1"
                     /translation="MRSRFMQGVAAALIVAALPAQSFATEPFAKVGTYALQFLKIGVS
                     ARGSGMGGAFTAISNDATATYWNPAGMVDITRTDVSLSYTWWPADIKLNYAAGAFTLP
                     FVPGTWGVSARALTMDPQVVRTVFLPEGTGEEFDAGDMSFGLSYAKFFTDKFSSGATV
                     HFIHSGLAEKSVNTFAFDFGLIYRIGLWGMRLGMMIQSLGAEVDYDDRASKMPTMFKV
                     AMAATPYSQGVNSLQGVFEFAHPSDNKERINLGAEYGFNQFFFLRGGYNVGTDSEGLS
                     AGMGLRIDTSQTSDLTFDYAWNDYDFLGDVHRISLGFTY"
     gene            10416..11948
                     /locus_tag="VEC56_08450"
     CDS             10416..11948
                     /locus_tag="VEC56_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXV14224.1"
                     /translation="MGAHVGAHVFFATALLCTLAGPAGAQHVGRGDFDFHLDSAAFRG
                     HDHRDLTHVFVRIPNHSLRFKEAGKAEWATRVKFSYLLTDDAGKEIVKQSETMTFSET
                     DASRVESSLAFQTVIKQFHLAPGGYWLSVSLENLDAPKISIIGLIKDKNKTSMVRRAR
                     LNLPEIPEDEPSFSTPLFVWTVDPREQGIRKYQPNAARMYGLYKDTLSVYVELYLPDS
                     MAKAPSFEFRTDIVDAKGESVRETKLTLPNPAPEGTSLRTYPVLLREDLTTLAAGTYS
                     LYLSFGLDGETIARVKSGDFSVAWDLRTWEIPRREYLAEARFLLGDDEFKAFQEKSPG
                     EQEQVLDALWKSFDPSPDSGGNEAYDLFVERMAYINAHFSERGPAVMTPQGDIYLRYG
                     PPDELVQDVIPLNYETLLEAEMVVDDPFHPMNFSSVGSKQYTLPVIRNTISGGSSNAR
                     SRPEDNTGVPYELWIYVAGGEPMLKRDKIQQMDVGMRFLFVDRDGHGVYTLERSSTIS
                     DK"
     gene            12041..12874
                     /locus_tag="VEC56_08455"
     CDS             12041..12874
                     /locus_tag="VEC56_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV14225.1"
                     /translation="MSRLVLAFVVLSLLPAGAASQGGIPRTQPIVATADSDSVATPVT
                     ESPAAADRSFLARMRGSALSQLYIKGGIVMHPLLLCAILGLVLIVERTWTLSRARVNT
                     RRLMVEVTQTLRTEGAEAALELCQRTRGPIAAILHAGLLRANRGPEAVEKAITSAGAI
                     EMAHLERGLLALASVSNVAPLLGFLGTVTGMIAAFAAIAAAEQVSAKLVAQGIEEALI
                     TTAAGLMIAVPVSAFYSYFVLQIDRFVLEMEEASADLVDELLDLEHPKGPGGRRPLDR
                     G"
     gene            12923..13330
                     /locus_tag="VEC56_08460"
     CDS             12923..13330
                     /locus_tag="VEC56_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV14226.1"
                     /translation="MERRRGRFRFGACEFQAASMSDLAFLLLVFFVVTTTFAVEEGIP
                     LVLPGRTSAPTRVEAEDVLEIRALANRRVEIQGQPVVLSDVRTLVATRLAANPELVVL
                     LSTHRDAEYGLMVDLLDEIKLANCRRISLRTVK"
     gene            13332..13772
                     /locus_tag="VEC56_08465"
     CDS             13332..13772
                     /locus_tag="VEC56_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV14227.1"
                     /translation="MRPTRFQRKHRVGNTIPTISMGDIAFLLLIFFMATTIFRLEQGL
                     QIVLPSAETAEKPRREHVARIWIDRTGRISIDDRLVTTADVEGIIKTVLMEAPEAVIA
                     LNADEKAPYGVVADVIEELKDANAARLSFTSDREERTVPPSRAP"
     gene            13932..15260
                     /locus_tag="VEC56_08470"
     CDS             13932..15260
                     /locus_tag="VEC56_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV14228.1"
                     /translation="MTFRRAIVAQMLILCALVPGARAEQYDLERCLELALEASTNVGV
                     SREQLRSARSDVLRSYGGLMPSLSLNMWAGHSWAGPTTGIVIDDQGRPIQPSGFDYEQ
                     YSFSISSQMNVFNWGANYNTINQSKRSADAAAYELEYTRDFVRAVTIREYYELVRQNK
                     LEEVQVANLEAKRRNLEQIEAFYKIGSRTKADYLQARVDYSNSELLLMNVRNAKAIAD
                     ARLKSRLNIPQDAPLEVDESIDFSASPVDLNAEVDYMFEHRSDLLASRYRIDAARAGL
                     SVTEKQRYPTIDASFNYGWNDRTFPGGGEVFKNNYSWSIGAFLNWNLFDRFQTKSGIQ
                     NARAQHRIAEYNLQQAKIDAVLDVKQIVLNIEQATERLDLAEETVAAAEENLRLAEER
                     YRVGAGTILETIEASSSLTSAQASLIDARVDYLVNRADLQRATGRSVTMP"
     gene            15348..16052
                     /locus_tag="VEC56_08475"
     CDS             15348..16052
                     /locus_tag="VEC56_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14229.1"
                     /translation="MLIHMIGIAKRYQMGSHVVNALDGVDLDVERNEYVALMGPSGSG
                     KTTFMNIIGCLDSPTEGTYVLNGENVTEMTDDTLAHVRNQEIGFVFQTFNLLPRASAF
                     QNVELPLIYAGMDAATRRERANESLEAVGLADRARHKPNELSGGQRQRVAIARALVNR
                     PSIILADEPTGNLDSKSGAEIMTLFDQLHGRGHTLIVVTHDDAVAAHARREIRLRDGR
                     IEVDRRRVATPTTKGA"
     gene            16054..>17075
                     /locus_tag="VEC56_08480"
     CDS             16054..>17075
                     /locus_tag="VEC56_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV14230.1"
                     /translation="MTRKRKVLIAVAGAVVLAALVGINLSAKRGKSIEVEAAKVGRRD
                     VSKVVTASGEIQPKRLVNVSANTIGKVTKLVVVEGQRVEKGDFLLEIDAAPYESQVEQ
                     LAAAVRAAEASVQLEDASLLKAEYDYERAVQLSEKGFMSENDLKTATSSLDIARARAR
                     SSREQLAQARASLKRAQHDLRQVHIVAEMSGVITALNVEEGESAIMGTINNPGTVLLT
                     IADLAEMEAEVKVDETEVVHVRVGQAATVRLDAQPDTTYRGVVTEVGNSALRTQVGLG
                     QESVDFKVVIAIQDSIPNIRPGLSASVDIAVAEERQVLAVPIQCLTVRDPERLARERV
                     RGGRKDG"
BASE COUNT         2983 a         5593 c         5583 g         2916 t
ORIGIN      
        1 cgcacaagct ggccggcatg atcggcgcgc tcgacggcta ctcgctccgc taccgggggc
       61 ctttcttccg cgagttcgtg gtggcgtgcc cgaccgacgc ggacgcgata atcgacgccg
      121 tgcgtaagca cggcgtcctg cccggcattc ccctctcacg ttactatggc ccggttgccc
      181 acaatctcct attggtaacg gccacggacc gacattccga cgaggatttc gagctacttt
      241 gtagcgcgct ggcgcgcgcc tctctcgtga aggtgggata gggaatggat cgcaaggatt
      301 tcgcgcagac catctatgga aagagccggc gtggccggca cggctaccac atctcgtcgc
      361 gcgcgacgga agcgccgcga gacatcccgc gcgagtacct gcgcgaagga cgcatcgggt
      421 tgcccgaggt ctccgagccg caggtgatgc gccactacgt cgacctgtcg atcaagaacc
      481 atcacatcga ccgcgacctg ttcccgctgg gcagttgcac gatgaaatac aaccccaagg
      541 tgaacgaaga cgtcgcggcc atgcccggct tcgccgggct gcaccccgag caaggcgacg
      601 acgacgcgca gggcgcgctc gcggtcatgc acgcgctcga gcaggcgctg atcgcggttt
      661 cgggaatgga cgcggtcagc gcgcaaccgg ccgcgggcgc gcagggcgag atgctggggc
      721 tcctgctcgc gcgcgcgtac ttcgccgacc ggggcgagtc gcgccacaag gtgctggtcc
      781 ccgacaccgc gcacggcacc aaccccgcca cggcgagcgt ggtcggcttc gagaccaaga
      841 ccttcgcgtc gggcaaggac gggcgcgtcg acctggacgt gctgcgcgcg gccatggacg
      901 acgatgtcgc cgtgctgatg ctcaccgtcc ccaacacgct gggcctcttc gaaaccagga
      961 tcgccgacat cgtcgatatc gtgcacaaga agggcgggct gtgctacatg gacggcgcca
     1021 atatgaacgc gctcatgggc cacgcgcggc cgggcgactt cggcttcgac atgatgcact
     1081 tcaacctgca caagaccttc tcgaccccgc acggcggggg aggaccgggc tcggggccgg
     1141 tggcggtgcg ctcgcacctc gagccgttcc ttcccacccc gcgcgtggtc gagagagacg
     1201 gtgggttccg tctcgagtac cagcgcccga agagcattgg tgcgttgcac tcgttcttcg
     1261 gcaacttcgg catctacctg cgcgcgtaca cgtacatcac gcgcctgggc tccgacggca
     1321 tccgccgcgc cagctcgacg gcgatcttga acgcgaacta cctcgccaag cgcgtgtcgt
     1381 cgcgtttccc ggtcccgtac ggcgagcgct gcatgcacga gttcgtcgcg acgggtgagc
     1441 ctttgaagaa gcacggcgtc aagacgctcg acgtcgccaa gcggctgctc gacttcgggt
     1501 tttatgcgcc caccatctac ttcccgctgg tggtgccgga agcgctcatg gtggagccga
     1561 ccgagaccga gagcaaggag acgctcgatc gcttcgcgga ggcgttgctc caaatcgcga
     1621 aggaagccgt cgaaaccccg gacgtgctga cgtccgcgcc gacgcgcacg ccggtgcgcc
     1681 gcgtcgacga agcgaccgcc aaccgctgcc cggtcatcac ggtgccgttg gattcgtagg
     1741 aggacatggg ctcgttcctc gctcgccgcg ccctgcaagc cgttctcctg gtcgcgatca
     1801 cgctgacgct caccttcgtt ctgctccacg tcgcccccgg cgacccgctc gcgcgctacc
     1861 tcgaccccag cgtcgacccc gccgacgtgg agcgcattcg ccactcactc gggctcgacc
     1921 ggccgctgca cgttcgcttc attcagtggg cgaaggcgtt cgtcgtcggc gacttcgggg
     1981 tgagcctcgc gcaccatcgc ccggtgcgag acctgctcgc cgaaaccatt ccgcgcaccc
     2041 tggtcctgac gtccgttgcg ctcgccgtcc aactcgtgct cggcatcctc gtggggacat
     2101 tggccgcgca ccaccgacgg cgcgcggtcg atcgcgtgct gtcgttttcg gtggtgctcc
     2161 tctacgctgt cccgtcctat tacctcgcgt atctgctgat cgaagtcttc gcggtcgacc
     2221 actcgtggct cccgacctcg ggcatttcga ctccgggcct gggtgcgacg ggcgccgtgc
     2281 tgttctggga ccacgtgcgt catctcgtgc tgcccgcggt ggtgctcggg atcgccagcg
     2341 cggccggctt cgcgcgcttc acgcgcggca gcatggtcga ggtactcgcc gaagactacg
     2401 tgcgcacggc gcgcgccaag ggactgcccg agtccagcgt ggtctggcgc cacgcgtttc
     2461 gaaacgcgct cggcgtgctg atcacactgg cggggctgag cgcgccgctg ctcttgggtg
     2521 gcgcggtgat cgtggagacc gtgttcgcct ggccggggat gggctcgctc atggtcgagt
     2581 ccatctacgc ccgcgactac ccggtggtgc tggcgatcaa tttcgtgggc gcctgcatgg
     2641 tgatcggcgg aaatttccta gccgatgtgg ccacgcagcg cgcggacccg cgtgccaccg
     2701 tcgagggagg cgctccgtga cgccgcggaa ccgggttccg ttcgcgagcg ccgcgtcccg
     2761 gcggggcgtt ggcgccgttc ttgcagcgat cctgtgcgcg tataccgctg tggttgtggt
     2821 tcgtttcggg gaggcggtcc gcctgccggt tgcggagcag ggggtcgtgc tggcggggtg
     2881 ggtgctgctc ctcggtggcg cggcctgggc cgcgcgcgcg acggggaggt tgcgacggcg
     2941 cttcgacgcc gacgcggtgg cgacgatcgc gctgtgcgtg ctcctctcga tggccgcgac
     3001 ctcggtgctg gcaccgctcg tcgtcggacc ggatccggcc gacgtcgcgc ccgccacgcg
     3061 ctatgacgcg ccgagcgcgg ggcacccgat ggggaccgac cgcttcggcc gcgacgtgtg
     3121 gtcccgggtg gtctacgggg ggcgcgcgtc cttcggcgtg tgcgcactgt cggtggcgct
     3181 cgcggtcgcg atcggaaccc tgttcggcgc gctgtcggga atggcgccgc gccggatcga
     3241 cgacgcgatg atgcgcacgg tcgacgggct gctgtcgttt ccgcgtctgt tgctcctgtt
     3301 gacggcggtg gcgttggtgc ccccaggcgc gggagccctc gcggtgttca tcgcggccac
     3361 gggttggatg ggcatggcgc gcatcgtgcg cggcgaagtc cggcgcatgc gctcgcgcga
     3421 gttcgtcgaa gcggccgtcg cgaccggtgt cggtagatcg cggctcttga cgaggcacct
     3481 gctccccaac gcgctgggcg cgctggtggt ggcggcgaca ctcaacgcgg ggaccgtgat
     3541 cctgatcgag tcgtcgcttt cgttcttggg attgggactc gccccgccca cgcccagctg
     3601 gggcgggatg gtgttcgagg gacgcgatgc gctctcgacc gcgtggtggg taagcgcgtt
     3661 cccggcgctc gcgatcacgg ccgcggtcac ggcgctgaat ctggcgggcg acggaatccg
     3721 cgacgcgctc gacgcgcgct cgtcgatgga gggatagcac gctcgcccgg tggctccgcc
     3781 caacgaatgg attgtagaaa acgatgaatc ggcgatacga tcagacgacc atggaagccc
     3841 aaggcaccga cccgcgtgcg accgcgctcg acgccgtcga cacgctgctg gacattcgcg
     3901 acctcacggt cagcttcgac acgcccgacg gccgcgcgcg cgccgtcgat cgcgtgagtt
     3961 ttaccattcg cgccggtgag acgctggggc tggtgggcga gagcggctgt ggcaagaccg
     4021 tctcggcgct ggcgctgatg cgcctgatcc agaccccgcc cgggcgtatc gagggcggcg
     4081 agatcctctt caaggggacc gacctcttga agctccccga gaaggacatg cgcaagatcc
     4141 gcggcaacga gatctcgatg gtcttccaag agccgatgac cagcctgaac cccgtgttca
     4201 cgtgcggcta ccaagtcgaa gaagcggtgg tgctgcacca gaaggtcagc aagcgcgaag
     4261 ccctcgagcg cacgctcgag atgctggcgc tggtcaagat tcccgacccc aagcgggtgg
     4321 ccaagtcgta cccgcaccag ctctccggcg gcatgcgaca gcgcgtgatg atcgcgatgg
     4381 cgctctcgtg caaccccagc ttgttgatcg ccgacgagcc gaccacggcc ctcgacgtca
     4441 ccatccaggc ccagattctc gagctcttgc agggactgca gcagaccttc ggcatggcga
     4501 tcctcatgat cacgcacgac ctcggagtga tcgccgagac ctgccgccgc gtcatcgtca
     4561 tgtatgccgg caaggtcatg gaggtcgcga gcgtaaacga cctattccac gatccgcgcc
     4621 acccgtacac gcgggggctg cgcgagtcca tcccgcgcat cgcgcacaag ggcgagcgcc
     4681 tcaaggtgat cccgggcaag gtccccgatc cgctcgacct gcccaagggt tgccgcttct
     4741 ccgatcggtg caagtacgcg gagtatcggt gcgacaacga agaggtccag ttgcgagacg
     4801 tgaaaccggg tcattcgatc cgatgctgga agaacattca agatgtctag cccgcaggtg
     4861 gaaatccagg ccggcgagcg cacgagcgcg cgacgcggcg aggtgctact caaggtcgac
     4921 aacctgtgca agcacttccc gattcgtcgc ggcgtgctgt cgcgggtggt ggggtggatc
     4981 cgcgcggtcg acggggttgg tttcgaagtc tatcgcggtg agacgctggg gttggtgggc
     5041 gagagcggct gcggcaagac cacgcttgcg cgcgcgatac tgcgcctgac cgacatcacg
     5101 tcgggcacgg tgacgttcga cgggattccg gtcttcgagc agaacgcgtc cgacatgcgc
     5161 aagctgcgcc ggcggatgca gatcgttttc caggacccct acggctcgct gaacccgcgc
     5221 ctgacggtgg gcagcatgat cgccgaggcg atcaagatcc acaagctcgc ggccggtgag
     5281 gcggtgcgcg agcgcgtcgc gtcgctgttg gatcgcgtcg gtattccggc cgagtacatg
     5341 aatcgctacc cgcacgagtt ctcgggcggc cagcgccagc gtatcggcat cgcacgcgca
     5401 ctcgcggtcg aacccgagct catcatcgcc gacgagccgg tcagcgcgct cgacgtgtcg
     5461 atccaggccc agatcatcaa tctcttgaag gatcttcagg acgcgctcgg gctgacctac
     5521 ctcttcatcg cgcacgatct cggcgtggtg gagtacatca gcgaccgcgt ggcggtcatg
     5581 tacctggggc gcatcgtgga actggcaccc gcgagcaagc tctaccgcga tcccaaacat
     5641 ccgtacaccc aggcgctctt gagcgcggtt cccagtttgg agcccggtgt gaagcgccag
     5701 cgtatcctgc tgcccggcga cgtacccagc cccgccgacg tgccgcgcgg gtgcgcgttc
     5761 catacccgct gcccgcacgc gatgtcgatc tgtcgggagc agacccctcc ccagcgcgag
     5821 gtcgagcccg ggcatcggac ggcgtgctgg ctgtacgaga agtagcgagg cgccgggaat
     5881 cttcgccggg cgggttgacg acacgaccga cccgggacat attggtggtg catccactgc
     5941 tggagcaccc tcccccaagt tccccacggg ccgagttccc acccagttcg gcccccgatg
     6001 ccggcagaag agtgctaatt cgttcgcgag ctttgagttc ggccgccgct gcggccccgg
     6061 gagtgcccga cggatgactt atattgacaa cccccggcga agtggtctat attgccgtcg
     6121 aagtcagtga tattgctagt attgcagcgg acggctgtga tagagtgacc ccacctgttc
     6181 gaagccgccg cggaggctgg gaccgccgcg gtgggatcgt ccccaccaca aaagattaac
     6241 ggcgtgttaa acacgacccc ggagagccgg ggcacgtgcg aagcgaaagg ggtttcgcga
     6301 atgttcccac gtgacagccg cggagcgatg atcgtcgcga tcgtcgtcgc gctcctggga
     6361 ttgacatcgt cggtggcgat ggcgcagacc ggtacggtta cgggaaccgt cgtctctgcg
     6421 aaggacaaca agcctctcgc gtacgccaat gtcatgatcg tcggcaccac catgggtgcg
     6481 atgagtctct ccgacggaaa attcgagatc aagggcgtgc ccatcggcac gtacaccgtc
     6541 aaggccatga tgatggggta cagcgcggtc gaacgagccg gcgtcattgt caatgccggc
     6601 gcggccgcga acgtggcctt caagctcgac gagaaagtcg tcgcgcagat ccaagaaatt
     6661 gtcgtgaccg cggacaaggc gatggtggaa gtcacctcga ccgaagtccg tagcggcgtt
     6721 acctccgagc aggtactcga catgccggtc gacgacgtcg tcgacgccat cggcctgaaa
     6781 gcgggcatcg tcaagaccgg cgacgacatg cacgtgcgcg gcggccgcag caacgaagtc
     6841 cagattcaaa tcgacggcgt tccggtgtcc gacccgctcg gcggcggctc cgtcgacgtc
     6901 ggcatgctgg gaacctccgg ctccgaaatc gtgtcgggcg gtatggacgc cgagtacggc
     6961 gacgcccagt cggccgttat caacatcgct accaaggaag gcggggccac cttcggcggc
     7021 gaagtgcgct acttcaccga cgacttcggc cgctccgaca agacctacac caacttcgac
     7081 cgtttgagcc tgggcttcgg cggaccgacc tggtggaaga atctgcgcta ctacctctcg
     7141 ggcgaagcga cctggagcga cacggagaac accaccatcg agccgcgtga agagcacaag
     7201 atcaccgact ggttcaagtt ccgccagcgc cagacgtcgg catacaacgt ccagggcaag
     7261 ctgacctaca agatggcgcg catgaaggtg gatggcgaaa tgatctactc gcagctgcgc
     7321 gaggacgatt atatcaacaa ctggaacatc gaaggctacg cgtcgaaggt gtggtacttc
     7381 cagggcctgg tcgtcgccgg cccgccggtg aacgggcgcg agcagttcca gttcgaccgg
     7441 ctgcgcagta tcaatcacgg cccgtgggcg gataccaagg atccggccaa gcaacaggcc
     7501 cagctcaacg tccggccggt cgacgtgatc gacttggttc gcgatcccaa caccggccag
     7561 cagaacgtcg tgacgtacac ggattttcgc gccgccgatt ttcgcgtcgg caatcagacc
     7621 attacggtgg tctgggacga ggcggtgcaa ggacccggcg gcgcgctttc gtacaagccc
     7681 tgggcgctgt tcgaaggctt ccagcgcggg gccagcgact tcaagccgtt cttgaccgct
     7741 ccccagggcg cggactccgc cttcgtgccc ttcaactcgg ccatgcacac gcccgagacg
     7801 cgctcggaga acctccagct caagcttggg ttcagccata acattacgag caagctgctc
     7861 tacacgctga agctctcgcg cctggacttg cgctccaaga gctcggtgct cgacgagttc
     7921 ggcgaccaga aagacccggc gcagttctcc accgcgggtc tcccggtgac cttgcccgac
     7981 ggcacgtacc tgaatgcggg cgtgtccaat gccgtgtggt acaccgacga cgacttcccg
     8041 tacttcgtga ccgcgtacga ctatccgttc tattccgacc agcactcgct gcagtacctg
     8101 ttcaagggcg acgtggtgtc ggaccagttc aaggggcacc gcatcaagac cggcgtccag
     8161 gtgctttaca acgacctcaa cgacgacgag cgcgtcaacc cggcgcagac ccgaatcgac
     8221 gagtacggga gaatccagca gggtctgaac gtgaatacgt acgagaactt caacgcggaa
     8281 ggcgcggtgt acgtccagga caagtgggac tacgaaggca tggtcgtcaa cggcggcttg
     8341 cgctacgagt ggttcacggt cggaaacaac agccaagtcc agatcgcgag cagcgagatc
     8401 gaccagtcgg tcgacgagtt caagtggaac ctttcgccgc gcttgggtgt ggcgttcccg
     8461 atcaccgacc gcgacaagtt cttcttccac tacgggcgct tcacgcagtg gccgagccgc
     8521 gtgtatctgt tccgcaccca ggacccggtg ggctcgacgg gaacactcgg caacccgaac
     8581 ctcgaacccg agctgacggt gtcctaccag gccggtatct cgcaccagtt caccgacgac
     8641 atcgccgcca acttcgtggt gttcaacaag gacatctacg ggctgatttc gtcgacgcgc
     8701 gtcaccgacg attccaccgg cgttcaggcc ttccggttca tcaaccggac ctacgcgagc
     8761 tcgcgcggtg tcgagttgtc gctcgagaag cgcctcacgc gccgcctcgg cttcgaggtg
     8821 tattacacct attcgttcgc ggacggcgtt gccagcgact ccgacttcgg ccgctccgcg
     8881 gagggcttga cccacttgcc gaccgaggaa tatcccctcg actgggacca gcgcaacacg
     8941 ttcaacgtga cgctgcgact gcaggatcgc aacaagtggg gcgccacgat gatctatcaa
     9001 tacggctcgg gtcttccgtg gaccccggtc gaccgcttcg cgcgcttgaa cgatcccacc
     9061 tgggagaact cgcgccgcct ggagccgacg cacaagctga gcatccaggg ccgcaagctg
     9121 ttcaacgtgt acggacgcga gctgacactc ttcttcgaag gccgcaatct gctggacgag
     9181 gacgtgctgt tgccgggcgg agacgccccc aacgcgttcc ccgggctggt tgcggcacaa
     9241 atggacggcg gttcatattt gaccgagacg ggggaatacg gcggcgcgta tctccaagac
     9301 caggacgccg acggccgaga cgacttcaac ccggtgttcg acccgacgat ttgggaaacc
     9361 caccgtctgt ggcggttggg cctcgggttc gagttctaaa tccggtacgc cggtatcctt
     9421 tcaggaggta ggaaagtgag atcaaggttc atgcagggtg tagcggcggc gctgatcgtg
     9481 gcggcgttgc cggcgcagtc gttcgccacc gaaccttttg caaaggtggg cacctacgcc
     9541 ctccaattcc tgaaaatcgg tgtgtcggcc cgcggctcgg gcatgggtgg tgcatttacc
     9601 gccatctcga acgacgccac cgcgacgtat tggaacccgg ccggcatggt cgatatcacc
     9661 cgcaccgacg tgtcgctctc gtatacctgg tggccggccg atatcaagct caactacgcg
     9721 gcgggcgctt tcacgttgcc gtttgtgccc ggtacctggg gtgtgagcgc gcgcgccctc
     9781 accatggacc cgcaagtggt tcgcacggtg ttcctgccgg aaggaacggg cgaggagttc
     9841 gacgcgggcg acatgtcgtt cggactgtcg tacgccaagt tcttcaccga taagttctcg
     9901 tcgggcgcga ccgtgcactt catccactcg ggcctggccg agaagagtgt caacaccttc
     9961 gcgttcgatt tcgggttgat ctatcgcatc ggcctgtggg gcatgcgtct cggcatgatg
    10021 atccagagct tgggtgccga ggtcgactac gacgaccgcg cttccaagat gccgaccatg
    10081 ttcaaggtcg cgatggcggc cactccgtac tcgcagggag tgaacagtct gcagggcgtg
    10141 ttcgagttcg cgcacccttc ggacaacaag gagcgcatca acctgggtgc cgagtacgga
    10201 ttcaaccagt tcttcttcct gcgcggcggt tacaacgtcg gcacggacag cgagggcctc
    10261 tctgcgggca tgggcctgcg catcgacacc agccagacgt cggacctgac gttcgactac
    10321 gcctggaacg actacgactt cctgggtgac gtccaccgca tctcgctcgg gttcacgtat
    10381 tgatcatgga tcggtcgcgg aaatactccg cacgtatggg cgcccacgtg ggcgcccatg
    10441 ttttcttcgc gaccgccttg ctgtgcacgc tggccggccc ggccggcgcg cagcacgtgg
    10501 ggcgcggcga cttcgacttc cacctcgact cggccgcgtt tcgcggtcac gaccaccggg
    10561 acctcacgca cgtgttcgtg cgcatcccca accactcgct gcgcttcaaa gaggcgggca
    10621 aggcggagtg ggcgacgcgg gtcaagttca gctatctgct caccgacgac gccggcaagg
    10681 agatcgtcaa gcagagcgag accatgacgt tcagcgagac cgacgcctcc cgggtcgaga
    10741 gctcgctcgc gttccagacc gtaatcaagc agttccacct ggcgccgggc ggctactggc
    10801 tctcggtctc gctcgagaac ctggacgcgc ccaagatcag catcatcggg ttgatcaagg
    10861 acaagaacaa gacctcgatg gtgcgccgcg cgcgcttgaa tctgcccgag attcccgagg
    10921 acgagccgtc cttcagcacg cccctgttcg tgtggacggt ggatccccgc gagcagggga
    10981 ttcgcaagta ccagcccaac gcggcccgca tgtacgggct ctacaaggac acgctgtcgg
    11041 tctacgtcga gctgtatctt ccggactcga tggcgaaggc accgagcttc gagttccgca
    11101 ccgacatcgt ggacgcgaag ggcgaatcgg tgcgcgagac gaagctcacg ctgcccaacc
    11161 cggctccgga aggcacctcg cttcgcacct atccggtctt gctgcgcgag gacctcacca
    11221 cgctggcggc cggcacgtat tcgctctacc tgtcgttcgg actcgacgga gagactattg
    11281 cccgcgtcaa gagcggcgac ttctcggtcg cgtgggactt gcgcacctgg gagatcccgc
    11341 gccgcgagta tctcgcggag gcgcgcttcc tgctcggcga cgacgagttc aaggccttcc
    11401 aggagaagag ccccggggag caggagcaag tcctcgacgc gttgtggaag agtttcgacc
    11461 cgtctcccga cagcggcggc aacgaggcgt acgatctctt cgtggagcgc atggcctaca
    11521 tcaacgcaca cttcagcgag cgcggacccg cggtcatgac gccgcagggc gacatctacc
    11581 tgcgctacgg gccgcccgac gagctggtcc aggacgtgat cccgctcaac tacgagaccc
    11641 tgctcgaggc cgagatggtg gtcgatgacc ccttccaccc catgaatttc agctcggtgg
    11701 gttccaagca gtacaccctg ccggtcattc gcaacaccat ctcgggcggg tcgtccaacg
    11761 cgcgcagccg ccccgaagac aatacgggcg tgccgtatga gctgtggatc tacgtcgccg
    11821 gtggcgaacc catgctcaag cgggacaaga tccagcagat ggacgtcggc atgcgatttc
    11881 tcttcgtcga ccgcgatggg cacggcgtct acacgctgga gcgcagttcc actatctccg
    11941 acaagtagcc cctttccccc ttgtgcgtcc gacggttgac aggcgagcgc cggcgcggct
    12001 atggtcgcgg acggttcgcc taaacgacca cctcaagacc gtgtcgcgac tcgtcctggc
    12061 attcgtcgtg ctctcgctcc tgcccgccgg cgcggcgtcg cagggcggta ttccgcgcac
    12121 gcagcccatc gtcgcgacgg ccgattccga ctcggtcgct acccccgtca ccgagtctcc
    12181 cgccgccgcc gatcgatcgt tcctcgctcg aatgcggggc agcgccctga gtcagctcta
    12241 catcaagggc ggcatcgtca tgcacccgct gctcttgtgc gccatcctcg gcttggtctt
    12301 gatcgtcgaa cggacgtgga cgctttcgcg cgcccgggtc aacaccaggc gactcatggt
    12361 cgaagtgacg cagacgttgc ggaccgaggg cgccgaggcg gccctcgaat tgtgccagcg
    12421 gacgcgcggg cccatcgccg ccattctgca cgccggtctc ctgcgcgcga atcgaggtcc
    12481 ggaggcggtg gagaaggcga tcacctccgc gggtgcgatc gaaatggccc acctcgagcg
    12541 tggcttgctc gcgttggcct cggtgtccaa cgtggcaccc ctgctcgggt ttttgggcac
    12601 cgtcaccggg atgatcgcgg ctttcgccgc gattgccgcc gccgagcagg tgtcggccaa
    12661 gctggtggcg caaggaatcg aggaggcgct catcacgacc gcggccggcc tgatgatcgc
    12721 ggtgccggtg tcggcgttct actcgtattt cgtgctccag atcgaccgct tcgtgctcga
    12781 gatggaagag gcaagcgccg acctggtcga cgagctcctg gacctcgagc acccgaaggg
    12841 ccccggcgga aggcggcccc tcgatcgcgg ctgacgcacc cgcgcgtggg gatgcgcggc
    12901 acgccgaagg aggtgccaca ccatggaacg acgcagaggg cgatttcgat tcggggcgtg
    12961 cgagttccag gccgcgtcga tgtcggatct cgcgttcctc ctgctcgtct tcttcgtcgt
    13021 gacgacgacg ttcgcggtcg aagaaggcat cccactggtg ctcccgggtc gaaccagcgc
    13081 accgacgcgc gtcgaggccg aagacgtgct agaaattcgc gctctcgcca atcggcgcgt
    13141 ggaaatacag gggcagccgg tcgtgctctc cgacgtgagg acgctggttg cgactcgact
    13201 ggccgccaac ccggagttgg tggtcctctt gtcgacccat cgcgatgccg agtacggcct
    13261 gatggtcgac ctgttggacg agatcaagct cgccaattgc cggcgcatct ccttgcggac
    13321 ggtgaagtga cgtgcgcccg acccgctttc agcgaaagca ccgcgtgggg aataccattc
    13381 ccaccatctc gatgggtgac atcgcgttct tgctcctcat cttcttcatg gccacgacca
    13441 tttttcgctt ggagcagggg ctgcaaatcg tcctgccgag cgccgagacc gcggagaagc
    13501 cgcgccgcga gcacgtggcg cgcatctgga tcgatcgcac cgggcgcatc tcgatcgacg
    13561 accggctggt gacgaccgcg gatgtcgagg ggatcatcaa gacggtcctg atggaggcgc
    13621 cggaggccgt gatcgcgctc aacgccgacg agaaggcgcc ttatggggtg gtggccgacg
    13681 tgatcgagga gctcaaggac gccaatgccg cccggctgtc cttcaccagt gaccgcgagg
    13741 aacggaccgt accgccgtcg cgagccccgt aggcgggccc ggaccttttt ttgaccctgc
    13801 cagcgggcgg tcgcggctat aatggcggaa cattcgaacc ccccccgccg tactacgttc
    13861 gttcgttttc acttgcgggc ggtcggcggc ggtggccgca tcttgttttt tgccaacggg
    13921 aaggtgacca gatgactttt cgccgggcga tcgtagccca gatgctgatc ctatgcgcgc
    13981 tcgtgcccgg tgcccgggcc gagcagtacg acctcgagcg gtgcctcgag ctcgccctgg
    14041 aggcctcgac caacgtgggc gtgtcgcgcg agcaattgcg gtcggcgcgc agcgacgtgc
    14101 tgcgcagcta cggcggattg atgcccagct tgagcctcaa catgtgggcg gggcactcgt
    14161 gggcgggtcc caccaccggc atcgtgatcg acgaccaggg ccgtcccatc cagcccagcg
    14221 ggttcgacta cgaacagtac tcgttctcga tctcgagcca gatgaacgtg ttcaactggg
    14281 gggccaacta caacaccatc aaccaatcga agcggtccgc ggacgccgcg gcctacgagc
    14341 tggaatacac gcgcgacttc gtgcgcgcgg tcaccatccg ggaatattac gagctcgtgc
    14401 gccagaacaa gctcgaagaa gtccaggtgg ccaacctgga ggcgaagcgg cgcaacttgg
    14461 aacagatcga agcgttctac aagatcgggt cgcgcaccaa ggccgattac ttgcaggcgc
    14521 gcgtcgacta ctccaacagc gagctcttgc tcatgaacgt acgcaacgcc aaggccatcg
    14581 ccgacgcgcg cttgaagtcg cgcctcaaca tcccgcagga cgcgccgctc gaagtcgacg
    14641 agtcgatcga cttctccgcc tcgccggtgg acttgaacgc cgaggtcgac tacatgttcg
    14701 agcaccgctc ggacctcttg gccagccggt accgcatcga cgccgcgcgc gccgggctgt
    14761 ccgtgaccga gaagcagcgc tatccgacca tcgacgcctc attcaactac gggtggaacg
    14821 accgcacctt ccccggcggc ggcgaagtgt tcaagaacaa ctactcgtgg tcgatcggcg
    14881 cgttcttgaa ctggaacctt ttcgaccgtt tccaaaccaa atcgggcatc cagaacgcga
    14941 gagcgcagca ccgcatcgcc gagtacaact tgcagcaggc caagatcgac gcggtgttgg
    15001 acgtgaagca gatcgtgctc aacatcgagc aggcgaccga gcgcctggac ctggccgagg
    15061 aaaccgttgc ggccgcggag gaaaacctgc gcctcgctga agaacgttac cgcgtgggag
    15121 cgggcaccat cctggagacc atcgaggcca gctcgtcgct cacctcggcg caggcgagcc
    15181 tgatcgacgc gcgcgtcgat tatctcgtca accgcgcgga cctgcagcgc gcgaccgggc
    15241 gcagcgtaac gatgccgtag acgcgccgtc ttactcacct tccgataccg cgcgcgggcg
    15301 gggcgctgct ttgcagcgct ccgcccgcgt gctatgctct cgaccccatg ttgatccaca
    15361 tgatcggcat cgccaagcgt taccagatgg gaagccacgt cgtgaacgcg ctcgacgggg
    15421 tcgacctcga cgtcgagcgc aacgagtacg tggccctgat gggtccgtcg ggatcgggga
    15481 agaccacctt catgaacatc atcggctgtc tcgattcgcc caccgagggc acctacgtac
    15541 tgaacggcga gaacgtgacc gagatgacgg acgataccct cgcgcacgtt cgcaaccaag
    15601 agatcggatt cgttttccag accttcaacc tgctgccgcg cgcgtccgcg ttccagaacg
    15661 tcgaattgcc gctgatctac gcgggcatgg acgcggcgac gcgccgcgag cgcgccaacg
    15721 agtcgctgga ggcggtgggg ctggccgacc gcgcgcgcca caagcccaac gagctctccg
    15781 gcgggcagcg ccagcgcgtc gccatcgcgc gcgcgctggt caaccgcccg tcgatcatcc
    15841 tcgccgacga accgaccgga aacctggatt cgaaatcggg cgccgagatc atgacgttgt
    15901 tcgatcaact gcacgggcgc gggcacacgc tgatcgtcgt gacccacgac gacgcggtcg
    15961 ccgcgcacgc gcggcgcgag atccggctgc gcgacggtcg tatcgaggtc gatcgccggc
    16021 gcgtcgcgac ccccacgacg aagggagcgt gaagtgacca ggaagcgcaa agtgctgatc
    16081 gcggtcgcgg gtgcggtggt gctcgccgcg ctggtgggca tcaacttgag cgcgaagcgc
    16141 ggcaagagca tcgaggtgga agccgccaaa gtgggccggc gcgacgtatc caaggtggta
    16201 acggcctcgg gcgagatcca gcccaaacga ctcgtgaacg tgagtgcgaa cacgattggt
    16261 aaggttacca agctggtagt ggtcgaaggt cagcgcgtcg agaagggcga cttcttgctc
    16321 gagatcgacg ccgcccccta cgagagtcaa gtcgagcagc tggcggccgc ggtccgcgcc
    16381 gcggaggcgt cagtgcagct cgaggacgcc agtttgctga aggccgagta cgactacgag
    16441 cgcgcggtgc agctgagcga gaagggcttc atgtcggaga acgacctcaa gaccgccacc
    16501 tcgtcgctcg acatcgcgcg cgcgcgtgct cgctcgtcgc gcgagcagct cgcgcaagcc
    16561 cgggcgtctc tgaagcgcgc gcagcacgac ctgcgccagg tccacatcgt ggcggagatg
    16621 tcgggtgtga tcaccgcgct caacgtggag gaaggcgaga gcgccatcat gggcaccatc
    16681 aacaatcccg gaacggtgct gctgaccatc gccgacttgg ccgagatgga agcggaggtc
    16741 aaggtcgacg agaccgaggt cgtgcacgtg cgggtcgggc aggcggccac ggttcgcctc
    16801 gacgcccagc ccgacaccac ctaccgcggt gtggtgaccg aagtgggcaa cagcgcgctg
    16861 cgcacgcagg tggggttggg gcaggagtcg gtcgatttca aggtcgtgat cgcgatccag
    16921 gattcgattc cgaacattcg tccggggctc tcggcttccg tcgacatcgc ggtggccgag
    16981 gagaggcagg tgctcgcggt ccccatccag tgcctcaccg tgcgcgaccc cgagcgactg
    17041 gcgcgcgagc gcgttcgcgg cggtcgcaag gacgg
//
LOCUS       DASPGK010000303         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_545655, whole genome shotgun sequence.
ACCESSION   DASPGK010000303 DASPGK010000000
VERSION     DASPGK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545655"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="mraY"
                     /locus_tag="VEC56_08485"
     CDS             <1..447
                     /gene="mraY"
                     /locus_tag="VEC56_08485"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXV14231.1"
                     /translation="LIMTYVAGNVKMATYLQVPYVAGAGELTVFCAAMFGAGLGFMWY
                     NCHPAQVFMGDTGSLSLGGSLGVVAVLLKRELLLVIIGGVFVAEALSVMIQVTSFKLT
                     RRRIFKMSPLHHHFELSGWHENKVVVRFWIVGALLVLLSLSTLKLQ"
     gene            450..1805
                     /gene="murD"
                     /locus_tag="VEC56_08490"
     CDS             450..1805
                     /gene="murD"
                     /locus_tag="VEC56_08490"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXV14232.1"
                     /translation="MVGFAGYSRFFVLGMARSGLAVSLLLARDGKRVAVFDDDAAVLE
                     KFRASEAARAYPSIEVISKDRAVAVAAASDCVVVSPGVASEHAVLAAARESRVPVMGE
                     IEAAYRYTRARIVGVTGTNGKSTTVGVIGDILKAGGVDAVVAGNVGTPLADVLRERDP
                     DTLVLELSSFQLDTIDRFRVDVAVLLNVTPDHLDRYHHSFDEYAASKARILNRAGAET
                     YYVYNAEDAVAAALAETHAGPAVPFSSRRRLDTGVYFDGEAIVRSWKGTREAVIRRSE
                     FTPVGVHNLENALASVAAVIPFEISLDAMRKALRGYRPLPHRMELVRVVDGVAYINDS
                     KATNVDATAKSLASIDGPVIVILGGRDKEGDFASLLPHLKTTRRAVLIGEARPVIARA
                     LSGKVEMSEATDMADAVRTARASAQPGDTVLLAPACASFDMFKNYQERGEVFRASVNS
                     L"
     gene            1818..>2284
                     /locus_tag="VEC56_08495"
     CDS             1818..>2284
                     /locus_tag="VEC56_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXV14233.1"
                     /translation="MDSTARTATADANGSDLLRLGMDPRLLVVTLFLMALGVSLVLSS
                     SSFFAGGKFGDHFALMRNHAARCVVALVVMFLASRVDYRVYRRAAPMLMLVGVLLMVG
                     VFVFGVTIRDTRRWYLLPLIHTTLQPSEIARTALVFFLAYWATRTGRDFRSFRR"
BASE COUNT          338 a          740 c          782 g          424 t
ORIGIN      
        1 ctgatcatga cctatgtggc cggcaacgtg aagatggcca cctatttgca ggtgccgtac
       61 gtcgccgggg caggcgaatt gaccgtattt tgtgctgcaa tgttcggtgc aggactcgga
      121 ttcatgtggt acaattgtca ccccgcgcaa gtgttcatgg gcgacaccgg ctcgttgtcg
      181 ctgggcggtt cgctgggtgt ggtcgccgtg ctgctcaagc gcgagctgct gttggtaatc
      241 atcggcggcg tgttcgtggc cgaagccctg agcgtgatga tccaggtgac gtccttcaag
      301 ctgacgcgcc ggcgcatctt caagatgtcg ccgctgcatc atcacttcga gttatcgggg
      361 tggcacgaaa acaaggtcgt ggtgcgcttc tggatcgtgg gcgcgctgtt ggtcctcttg
      421 agcttgagta cgctcaagct gcaataggca tggtcggatt cgccggatac tcgcgtttct
      481 tcgtgctcgg catggctcgc agcggcctgg ccgtgagcct cttgctcgcg cgcgacggca
      541 agcgcgtggc ggtattcgac gacgacgcgg ccgtcctgga gaagtttcga gcgagcgagg
      601 ccgcgcgcgc gtatccgagt atcgaagtca tttccaagga tcgcgcggtc gcggtcgcgg
      661 cggccagcga ttgcgtggtc gtgagcccgg gggtcgcctc cgaacacgcg gtcctcgcgg
      721 ccgcgcgcga gtcgcgcgtg cccgtgatgg gcgagatcga ggccgcgtat cgctacaccc
      781 gcgcgcgcat cgtgggtgtt accgggacca acggcaagtc cacgaccgtg ggcgtcatcg
      841 gcgacatttt gaaggcgggc ggcgtcgacg ccgtggtggc cgggaacgtc gggaccccgc
      901 tcgcggatgt actgcgcgag cgcgatcccg atacgctggt gctcgagctg tccagctttc
      961 agctcgacac catcgaccgg ttccgcgtcg acgtcgccgt gcttctcaac gtcacccccg
     1021 accacctgga tcggtaccac cactccttcg acgaatacgc ggcgtcgaag gcgcgcatct
     1081 tgaaccgcgc gggggccgag acctactacg tgtacaacgc cgaggacgcc gtggcggccg
     1141 cgctggccga aacccacgcg gggccggcgg tcccgttcag ctctcggcgc cgtctcgata
     1201 ccggcgtcta tttcgacggc gaggcgatcg tgcgcagctg gaagggcacg cgcgaggccg
     1261 tgatccggcg cagcgagttc actccggtcg gcgtgcacaa cctggagaac gcgctcgcgt
     1321 cggtggccgc ggtgattccg ttcgagatct cgctcgacgc gatgcgcaag gcgctgcgcg
     1381 ggtaccgccc gctcccgcac cgcatggagc tggtacgcgt ggtcgacggt gtggcctaca
     1441 tcaacgactc caaggccacc aacgtcgacg ccaccgccaa gagcctggcc agcatcgacg
     1501 gccccgtgat cgtgatcctg ggcggccgcg acaaggaggg cgatttcgcc tcgctgctgc
     1561 cgcacctgaa gacgacccgg cgcgcggtgc tgatcggcga ggcgcgcccc gtgatcgcgc
     1621 gcgcgctgtc gggaaaggtc gaaatgagcg aagcgacgga catggccgac gcggtgcgca
     1681 cggcgcgcgc gtcggcgcaa ccgggcgaca ccgtgttgct cgcgcccgcg tgcgcgagct
     1741 tcgacatgtt caagaactac caggagcgcg gcgaagtgtt ccgcgcgagc gtcaactctc
     1801 tttagaaaga ggagcgcatg gactccaccg cccgcaccgc caccgccgac gccaacggat
     1861 cggacctcct gcgtctggga atggaccccc ggctgctggt ggtgacgttg ttcctgatgg
     1921 cgttgggcgt gagcctggtg ctctcctcca gctcgttctt cgcgggcggg aagttcggcg
     1981 accacttcgc gctcatgcgc aaccacgccg cgcgctgtgt ggtggcgctg gtggtcatgt
     2041 tcctggcctc gcgcgtcgac taccgggtgt atcgccgggc agcgcccatg ctgatgctcg
     2101 tgggcgtgct gctgatggtg ggcgtgttcg tgttcggcgt caccattcgc gacacgcggc
     2161 gctggtatct cctgccgctc attcacacca cgctgcaacc ctcggagatc gcgcgtaccg
     2221 cgctcgtgtt cttcctggcc tactgggcca cgcgtaccgg ccgcgacttc cgttcgttcc
     2281 ggcg
//
LOCUS       DASPGK010000304         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1717415, whole genome shotgun sequence.
ACCESSION   DASPGK010000304 DASPGK010000000
VERSION     DASPGK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717415"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1503)
                     /locus_tag="VEC56_08500"
     CDS             complement(223..1503)
                     /locus_tag="VEC56_08500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14234.1"
                     /translation="MGRHGIALQAQLIVRTLRATLTALVLTGPLAATSAAEEATPMET
                     VETLPRDAKLALFEAQQALEEGKSEKAVDILGTYVRDHAKKNDSFLMRYHYASMLVQV
                     DRREEALAEYERVVALEPRYDAGWLGLGETAYGLGQFQRAAEALKKGYQASAEKRPDV
                     LYYAAAAQLLGGDAAGAVPVLEDLVSGKHGEPKFEWYRGLVSACLQAEDHTRGRKAVD
                     DMIERFGTNADAWYLAFQFAASVSDYHQAAVALTVVGYLRPLTRQEQLQLGDLYAAVE
                     APAAAAGYYSAATQDTASAGEVERVASAYLASYQSEAALKVLEQGIQDEPTFRLWSLL
                     GDLHVMESRYEQAEAAFAECVRLKPDEPRPHLMLGYCMIELGRPDDALAPLAVAASNE
                     EWAERAQMLVKRAQIMRAAPPSAPEPTATSAPAP"
     gene            complement(1467..2105)
                     /locus_tag="VEC56_08505"
     CDS             complement(1467..2105)
                     /locus_tag="VEC56_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV14235.1"
                     /translation="MFRLPKWVPIALAFGLAVNVLLLTGAAVLSGEADGQEQDITAPM
                     AVNTLNFEQTPPPAEEAAAEPEAPKREEARPDLGPDLFQPDFAPDFGGMGGVGDAIAI
                     DLTGGANTAMKSDFVFESYELDQAPRAVVKMPPQYPFKAREKGIEGVVQVRVLVKEDG
                     TVGEVLIMDARPPDTFEDAVLMAVPRWRFEPGVVGGKKVTSWVVTALHFKLN"
     gene            complement(2112..2528)
                     /locus_tag="VEC56_08510"
     CDS             complement(2112..2528)
                     /locus_tag="VEC56_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV14236.1"
                     /translation="MINVRKTIRGHEQIHIDVINLVDVMMVLLIFFVATTSFVKETGI
                     DVQRSTAATAEVKDQGNVTIGLSADGSIFMEGKRVDIRRVRGLVERALAEDPKTGVVV
                     IADRDSKTSDVVALMDQCRMAGARNVSLAARRAWGS"
     gene            complement(2544..3134)
                     /locus_tag="VEC56_08515"
     CDS             complement(2544..3134)
                     /locus_tag="VEC56_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV14237.1"
                     /translation="MELILRLLEFAKTGGWVMVPVAVTSVLMWTLIGERLYTYRRLGR
                     RDLPIQDAVHAVEGAPVNHFGHGLRATLVRNFVRKRTGEADLDKRILRECAMRIEPVI
                     DRNLAVIAVLASIAPLLGLLGTVSGMITTFVVIAELGTGNARPLAGGISEALVATQAG
                     LLVAIPGLFASVALKRRADKLAHRLDQTTLLLQHHV"
     gene            complement(3109..>4393)
                     /locus_tag="VEC56_08520"
     CDS             complement(3109..>4393)
                     /locus_tag="VEC56_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV14238.1"
                     /translation="EAKITSDRAALRAEVDKLEALGKQLTADVQALQKRKVAGQERHD
                     ALTEKWTTKEVEFREISGNVRIAAKDIEALLNTSQLTALFPDRLNTVAPVLEPGYFPG
                     IDDISGMTDVLLDEMKRSGQVTLQQGPYVGRDGENHTGDILTLGKFTALYRSPEEVGF
                     LTYNTDSKKLFALTNLPARKMTKTIAHYFDGKSDVVPLDIASGESLVGIGDHTSIVEE
                     FKQGGNIMWPILLVGLVVVVFTSYKLIYLKRVHGDTDTIMGHVNDLAASGDWPGVDAI
                     MQKHQNHNWPVVNVLRDGIRARNEDRETLESVLQESILREMPRLERGVSVMAVLGAIA
                     PLLGLLGTVTGMITTFQVITLYGTGDPKLMSSGISEALVATKWGLCVAVPTMLIHTLL
                     ARRVHGVVSDMEEKAVTLSNTIQKEQRRGTHIASA"
BASE COUNT          700 a         1472 c         1415 g          806 t
ORIGIN      
        1 catacccgtc gacaacaacg caaatgattt gcgaatcatt ctcggttccc tcctcggtag
       61 cccatgctgc tcacttaata tgagtcgctc gacagaatac gtgacgcgga cgataggcgg
      121 gcaatgtaaa ggcagcatta agcgccctta aaggttcgat ggagaaaccg taacccgttt
      181 cagggcttgg ctttgaaccg taaagagggg cacggattcc gctcagggcg cgggtgccga
      241 ggtggccgtg ggttccggtg ccgacggcgg cgcggcgcgc atgatctgcg cgcgcttgac
      301 cagcatctgc gcgcgctccg cccattcttc gttggacgcg gccaccgcga gcggcgccag
      361 cgcgtcgtcg gggcggccca actcgatcat gcagtagccg agcatgaggt gcggcctcgg
      421 ctcgtcgggc ttcaagcgaa cgcactcggc aaacgcggcc tcggcctgct cgtagcgact
      481 ctccatcacg tgcaggtcgc cgagcagcga ccacaggcga aacgtcggct cgtcctggat
      541 tccctgctcg agcaccttga gcgccgcctc ggactgatag gacgcgagat aggccgacgc
      601 tacacgctcg acttcgccgg ccgacgcggt gtcttgcgtc gctgcggagt agtagcccgc
      661 cgccgcggcc ggtgcctcga cggccgcgta cagatcgccc agctgcagct gctcctgccg
      721 cgtcagcggg cgtagatatc caaccacggt gagcgccacc gcggcctggt ggtagtcgct
      781 cacgctggcg gcaaattgaa acgcgaggta ccacgcgtcg gcgttggtgc cgaagcgttc
      841 gatcatatcg tcgaccgcct tgcgcccgcg cgtgtggtct tcggcctgga gacacgcgct
      901 taccagaccg cggtaccatt cgaacttggg ctcgccgtgt ttgccggaca ccagatcctc
      961 cagaaccggg acggcgcccg cggcgtcacc gcccaagagc tgcgcggcgg ccgcgtagta
     1021 gaggacgtcc gggcgcttct cggcgctggc ctgatagccc ttcttgaggg cctcggcggc
     1081 gcgctggaac tgccccaggc cgtacgccgt ctccccgagt cccaaccaac cggcgtcgta
     1141 acggggctcg agtgcgacca cgcgctcgta ctcggccagc gcctcctcgc gacgatccac
     1201 ttgcacgagc atgctcgcgt agtggtaccg catgaggaag ctgtcgttct tcttggcgtg
     1261 gtcgcgcacg tacgtgccga gtatgtcgac tgctttctcg ctcttgccct cttcgagcgc
     1321 ctgctgggcc tcgaacagag cgagctttgc gtcgcgcgga agcgtctcga cggtctccat
     1381 cggcgtggct tcttcggcgg cgctcgtcgc ggccagcggg ccggtgagca ccagcgcggt
     1441 cagcgtcgcg cgcagggtgc gcacgatcag ttgagcttga agtgcaatgc cgtgacgacc
     1501 cacgaggtca ccttctttcc gccgaccacg cccggctcga atcgccagcg cgggacagcc
     1561 atcaataccg cgtcttcgaa cgtgtcgggc ggacgcgcgt ccatgatgag cacctcgccc
     1621 acggttccgt cttctttcac caggaccctg acctgaacca cgccctcgat ccccttctcg
     1681 cgcgccttga acggatactg gggcggcatc ttgacgaccg cccgcggcgc ctggtcgagc
     1741 tcgtacgact cgaacacgaa gtcgctcttc atcgcggtgt tcgcaccgcc cgtgagatcg
     1801 atcgcgatgg cgtcgcccac cccgcccatg ccgccgaaat cgggggcgaa gtcgggttgg
     1861 aatagatcgg ggcccaagtc cggccgggcc tcttcgcgct tgggtgcctc cggctccgcc
     1921 gcggcttctt cggcgggcgg cggcgtctgc tcgaagttca gcgtgttgac cgccatggga
     1981 gcggtgatgt cttgctcctg gccgtccgct tcgcccgaca acaccgcggc gccggtcaag
     2041 agcagcacgt tgaccgcgag gccgaacgca agcgcgatcg gaacccattt cggaagccga
     2101 aacatgtcgc gctacgaccc ccacgcgcgc cgcgcggcca ggctgacgtt ccgggcaccc
     2161 gccatgcggc attggtccat caacgccacc acgtcgctgg tcttgctgtc gcggtcggcg
     2221 atcacgacga ctcccgtctt ggggtcttcg gccagcgcgc gctccaccag cccgcgcacg
     2281 cgccggatat cgacgcgctt gccttccatg aagatgctac cgtcggcgga caagccgatg
     2341 gtgacgttgc cttgatcctt gacctcggcg gtggcggcgg tcgagcgctg cacgtcgatg
     2401 ccggtttcct tgacgaacga cgtggtcgcg acgaagaaga tcaggagcac catcatcacg
     2461 tcgaccaggt tgatgacgtc gatgtggatc tgctcgtggc cgcgaatggt cttgcgaacg
     2521 ttgatcatgt tgtttccttg ttcctagacg tgatgctgga gcagcagcgt ggtctggtcc
     2581 agccggtgcg ccagcttgtc ggcgcgtcgc ttcagggcca cgctggcgaa cagcccgggg
     2641 atcgccacca gcaggccggc ctgcgtcgcc accagcgctt cggagatccc tcccgccaag
     2701 ggccgcgcgt tgccggtgcc gagttcggcg atcacgacga aggtcgtgat catgccgctc
     2761 acggttccca agagccccaa caagggggcg atcgacgcga gcaccgcgat cacggccagg
     2821 ttgcgatcga ttacgggttc gatcctcatc gcgcactcgc gcaggatgcg cttgtccagg
     2881 tccgcctcgc cggtccgctt gcgcacgaag ttgcgcacca gtgtggcccg cagcccgtgg
     2941 ccgaagtggt tgacgggcgc gccctcgacc gcgtgcacgg cgtcctggat cggaaggtcg
     3001 cgacggccca ggcgccggta cgtgtacagg cgctccccga tcagcgtcca catcaggacc
     3061 gaggtgacgg ccaccgggac catcacccag ccgccggtct tggcgaactc aagcagacgc
     3121 aatatgagtt ccacgacgct gttccttctg gatcgtgttg ctcaacgtca cggccttctc
     3181 ctccatgtcg ctcacgacac cgtgcacgcg acgcgcgagg agggtgtgga tcagcatggt
     3241 cgggaccgcg acacacaggc cccacttggt cgcgaccagg gcctcgctga tgccgctcga
     3301 catcagcttg gggtcgccgg tgccgtacag cgtgatcacc tggaaggtgg tgatcatgcc
     3361 cgtcacggtt cccaacagac cgaggagcgg cgcgatggca ccgaggacgg ccatcaccga
     3421 aaccccgcgt tcgaggcgcg gcatctcgcg cagaatggac tcctgcaaga cgctctcgag
     3481 cgtctcgcgg tcctcattgc gggcccgaat accgtcgcgg agcacgttca ccacgggcca
     3541 gttgtgattc tggtgcttct gcatgatcgc gtcgacgccc ggccagtcgc cgctggcggc
     3601 gaggtcgttg acgtggccca tgatggtatc ggtgtcaccg tggacgcgct tcaagtagat
     3661 cagcttgtag ctcgtgaaca ccaccaccac cagcccgacc agcaggatcg gccacatgat
     3721 gttgccgccc tgcttgaact cctcgacgat gctggtgtgg tccccgatgc ccaccagcga
     3781 ctcgccgctt gcgatgtcga gcggaacgac gtcggacttg ccgtcgaagt agtgggcgat
     3841 cgtcttcgtc atcttgcgcg cagggagatt ggtcaacgcg aagagcttct tgctatcggt
     3901 gttgtaggtg aggaacccaa cctcttcggg cgaccggtac agggccgtga acttgcccag
     3961 cgtcaggatg tcgccggtgt ggttctcgcc gtcgcgtccg acgtaggggc cctgctgcag
     4021 ggtcacctgg ccggagcgtt tcatttcgtc caagaggacg tcggtcatac cgctgatgtc
     4081 gtcgatgccc gggaagtagc ccggctcgag cacgggcgcg accgtgttca gccgatccgg
     4141 aaacaatgcc gtcagctgcg aggtattgag cagcgcctcg atgtccttgg cggcgatgcg
     4201 cacgttgccc gagatctcgc ggaactcgac ttctttggtg gtccacttct cggtcagcgc
     4261 gtcgtgacgc tcctggcccg cgaccttgcg cttctggagc gcttgcacgt cggcggtgag
     4321 ctgcttgccc agagcctcga gcttatcgac ctcggcgcgc agcgcggcgc ggtcgctcgt
     4381 gatcttggcc tcg
//
LOCUS       DASPGK010000305        10099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1248249, whole genome shotgun sequence.
ACCESSION   DASPGK010000305 DASPGK010000000
VERSION     DASPGK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248249"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VEC56_08525"
     CDS             <1..1324
                     /locus_tag="VEC56_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXV14239.1"
                     /translation="FTADGQLFYPSAPAMPGQPDPSHLPEFFGDFILVNSAPWPILDV
                     EPRQYRFRMLNGSDSRFYELWLQEKTGNDAVPIKAALPSMYQIGTDTGLLNAPVALTR
                     FVIGTGERADMVFDFSDPTLWGKTFILRNSGKTPFPKGSAVNPVTAGQVMAFRISKPL
                     DTTTYPATTLPANLRPLHGPVGMPVANADTRELILFEGTDEYGRLQAKLGTLDGALAW
                     QAPITENPMVNDTEVWEIYNTTADAHPIHLHLVSFRIVDRQRFKADVDPVTGRVSNVR
                     LVGRPMVPPAREDGWKDTAVMFPGEVTRVVATFDREGLYVWHCHILSHEDHEMMRPYY
                     VRPMAGAPEAAVADTPLRIASVTPNPFNPSTRIHFEVSRPLTVQAAIYDVAGRLVRRF
                     DDRAVAAGAHVLEWNGSTDAGASVATGVYFLQLRAGSAVETRRLVMIK"
     gene            complement(1416..2891)
                     /locus_tag="VEC56_08530"
     CDS             complement(1416..2891)
                     /locus_tag="VEC56_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HXV14240.1"
                     /translation="MNEIKDSLLGNALQGADTAALSSFGAYSLLFAPGLVSGLRLRAS
                     RARAQAAFWNAHRRVPAYRDFLREHAAERPRRFEDVPPMDKDGYIRRHPLESRCQFGR
                     FPSRDAVIDESSGSSGKATSWVRGPAEREATRRLLQFGVRHTFGKEPFILLNAFALGP
                     WATGMNVSMSLVEQCVLKSVGPDQAKLEAALQDLGPGYRYLITGYPPFLKQFVDHADI
                     DWKKYRIHAAVGGEGMSEGLRAALNRRFQRTISSFGASDLEINMAVETDFTIALRQEL
                     ARNADLARDLYGSEGLPMVFQYDPLSYYIESDGERNLLFTLNRLDNVSPRIRYNLYDR
                     GIAMRCRNALEMLRDHRVALDPPAPRVNLPLLFHWGRREHAVAFYGCKITPDDVQNAI
                     TRVPLLQELTANYALHPYEDDAANKRLEIWIELSAESQAPDDVAGITEGLKRELAAVN
                     QDFREAIRMIQPGFHPILRFFPHGEGPLAGQDPRVKRRYIL"
     gene            complement(2884..3876)
                     /locus_tag="VEC56_08535"
     CDS             complement(2884..3876)
                     /locus_tag="VEC56_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14241.1"
                     /translation="MMYDAWNRLSRIARYCPTPHNTQPFRIRPTSEVEADLVCVTSRL
                     LPREDHGNLYCMAAFGMFVEALLRSGRHLGLDVDVTAEPGVDPATVHQGPGASVRLGR
                     ASIRGTCEAHDQPELLDTRRTSRLPYDGRPVSAQALADLRACACNAGHRFSSYDDDET
                     VTWVLNTNVEAIIDNLQIAPERMEIQGWYRTGPTPEHGDGLWQLPMNQSKLEMGAAFF
                     APRFLKLPGVRHLARRRFLATQKGTRHVALLCGPFSTWDELLRAGRMLLEFWIIMARH
                     RVYMHPYGSMLTNPVYAREVADRFGANDAWLILRMGYSPVPPRAPRLASIIVHE"
     gene            complement(3873..4424)
                     /locus_tag="VEC56_08540"
     CDS             complement(3873..4424)
                     /locus_tag="VEC56_08540"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV14242.1"
                     /translation="MVAFHQARLEWLTAMDMHVAVTVGCVAPPEDLFRHAVTDLETLP
                     RGVRRCGPLPGVARAEILEGDALRTGVVRRVHLTDGSVLNEGIVEIAAPSSMSYRQLT
                     PYRFPLSLLAKSARGRYTFTPTGSGTRMTWEATIQLTSPLAYPLMACVRGLFLRRMMT
                     GFLECVRDDVVRAQSNRDGSTAS"
     gene            4575..5855
                     /locus_tag="VEC56_08545"
     CDS             4575..5855
                     /locus_tag="VEC56_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV14243.1"
                     /translation="MKKKPVLIGSAVVVVAVVVVGALSMRGRGEQAIQVQTAQVSRQK
                     IVQKVNGTGKIQPETMVKISADVSAKITRLAVVEGQWVEKGAFLLELDRERYLALVES
                     AEAVVRSAEAEAKLVKENLGRAEKEFNRSKELAGRDLESQSIYDDREAAYQVELARYD
                     SAKNQVSQARAALKQARDDLSKTTIYAPMAGTISDLMKEQGEIAIGSQFQPDVILVIA
                     DLSVMEAQINVDENDIVSVALGQEAEIEVDALPDQVLKGVVSEIASSANSTGAGSTDQ
                     KTEFEVKITVTSPPQTLRPGMTATADIFTKTNDDALSVPIQSVAARTVDQLTVAGQTR
                     EEAEKVFTTDKDGFVEIVFCIEEGKAVAKQVKTGIQSDDLIEITEGLSEGEEVVTGSY
                     RAISKDLNNGGAVTVENDEKPDAKEKTEQASASN"
     gene            5920..6573
                     /locus_tag="VEC56_08550"
     CDS             5920..6573
                     /locus_tag="VEC56_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14244.1"
                     /translation="MGADTVQALRGVSLDIQRNEYVAVMGPSGSGKSTLMNVIGCLDV
                     PTSGTYALEGQMVATMSEYQLADVRNQRIGFVFQTFNLIPRSNVFHNVELPLIYAGVA
                     KARRRERVERAIERVGLSNRVKHKPNELSGGQRQRVAIARALVANPSIILADEPTGNL
                     DSKTGDEIMGLLDELHAAGQTIILVTHEDYIAEHAHRVVRLRDGMIESDERASPREG"
     gene            6576..7808
                     /locus_tag="VEC56_08555"
     CDS             6576..7808
                     /locus_tag="VEC56_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14245.1"
                     /translation="MRWRIDLEESLAIALTALRANKARGVLTTLGIIIGIVAVILTMT
                     AAHGLQSAFRQSFSAVGSDVIYVSRFPWIIMNDFFLYRNRPPMDLHDAGALAERLSGK
                     AIVNPTLGGQQAVQYRGETMDAVQIIGTTEKRTLVASGQADRGHFLTDFEVQYKKNVC
                     VIGTEIEKALFQGTDPLGKEIKIGRTAFRVVGVMEKQGGGFLGGPSFDRQIYVPISSY
                     IRAYGGERLDVNLAVKAPSQEALGDLEYEVIGEMRKIRGLRPSEKDDFSINKLDTLVG
                     TFDNVMGVVLLVGLLITSISLFVGGVGVMNVMFVSVTERTREIGIRKAIGAKPHSILF
                     QFLFESAAICLIGGFIGIAIATLLTGVLNATVMPASVSPGILFTAVVVALAVGAIAGF
                     VPALKGARMNPVDALRYE"
     gene            7816..9060
                     /locus_tag="VEC56_08560"
     CDS             7816..9060
                     /locus_tag="VEC56_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14246.1"
                     /translation="MRASDLLGMSTGAIVGSKLRSSLTLLGIVAGVASIIAVMTAIQV
                     VQSAMEHEMSVLGTQTFQVQKWPAGFSSDEERRKARTRPPLTVDNADAIRAQVAMVDI
                     VGSELWDFGFKAEYGGEETNSNLSIVGGTPEYPQNNTHYVGGGRNISQIDVKAGRRVA
                     VIGHAIGKTLFPFTDPVGKELRLDGRKYDIVGVFDEKKSAFGSGYDNYVLIPITTFQS
                     IYGSTTRDGFPRSVNITVHAKTPEMIDDAIEETRQVLRRERRVAPGEPDNFDFFSSQS
                     LITEFDKLTAKIKIAAFVVSIVALIVAGIGIMNIMMVSVTERTKEIGIRKSLGANPRN
                     ILIQFLLEAVILCNIGGLVGIVVGFGLGNVITFFTSFEVEVPLQWAVIGLVFCSTVGI
                     AFGLLPAIRASRLHPIDALRYE"
     gene            9128..>10099
                     /locus_tag="VEC56_08565"
     CDS             9128..>10099
                     /locus_tag="VEC56_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXV14247.1"
                     /translation="MATPDFEKLGVFYLGREYDVARRAASDDLVLYDSKDLTTHAVCI
                     GMTGSGKTGLCIGLLEEAAIDGIPAIVIDPKGDLVDLALAFPNLDATSFRPWVNEDSA
                     RQQGMTPDEFAAREADKWAKGLAQWGQDAARIRRLRDAADVVVYTPGSDAAIPVSVLS
                     SLECPKGDASADLLAERASGVASSLLSLLGIEADPVRSREHILLTTILQSAWSQARSL
                     DLAGLVQAVQSPPVARVGVMELESFFPAKERFALAMLVNNLLAAPRFQSFLAGDPIDI
                     DRMLYGETGKPRVAVFSIAHLDDAERMFFVSLLLNEVVGWTRTRSGTS"
BASE COUNT         1836 a         3233 c         3336 g         1694 t
ORIGIN      
        1 gttcacggcc gacgggcagc tcttctaccc ttcggcgccg gcgatgccag ggcaacccga
       61 cccgagtcat ctccccgagt ttttcggtga cttcatcctc gtcaacagcg cgccctggcc
      121 gatcctggat gtcgagccgc gccagtatcg cttccgcatg ctcaacggct ccgattcgcg
      181 cttctacgag ctgtggctcc aggagaagac gggcaacgac gccgtgccga tcaaggccgc
      241 gttgccgtcg atgtatcaaa tcggtaccga caccggtctg ctcaacgcac cggttgcgtt
      301 gacgcgcttc gtgatcggga ccggcgaacg cgccgacatg gtgttcgatt tctcggaccc
      361 gaccttgtgg ggaaagacat tcatcctgcg caacagtggg aagacgccgt tcccgaaggg
      421 gagcgctgtg aacccagtca ccgcgggtca ggtgatggca ttccggattt ccaagccctt
      481 ggatacgacc acctatccgg caacgactct cccggccaac ctacgtcccc ttcacgggcc
      541 ggtgggcatg ccggtcgcga acgccgacac ccgcgagctg atcctgttcg agggcacgga
      601 cgaatacggg cgtctgcaag ccaagctggg aacgctcgac ggcgccctcg cctggcaggc
      661 gccgatcacg gagaacccga tggtgaatga caccgaggtg tgggagatct acaacaccac
      721 cgcggatgcg catcccatcc acttgcacct cgtctccttc cgcatcgtcg accgccagag
      781 gttcaaagcg gacgtcgatc cggtcacggg acgcgtgagc aacgtgaggc tggttggacg
      841 tccgatggtg cctcccgcgc gggaggacgg atggaaggat acggccgtca tgtttccggg
      901 tgaggtgacg cgcgtggtcg cgaccttcga ccgcgagggg ttgtacgtgt ggcactgcca
      961 cattctctcg cacgaggatc acgagatgat gcggccctac tacgtgcgac cgatggcggg
     1021 ggctccggaa gccgcggttg cggacacacc gctgcgtatc gcgtcggtca cgccgaaccc
     1081 gttcaacccg tcgacgcgaa tccacttcga agtgtcgcgt ccgctgactg tgcaggcggc
     1141 gatctacgac gtggcgggac gattggtccg ccggttcgac gaccgcgcgg ttgcagccgg
     1201 cgcccacgtg ctggagtgga atggttccac cgatgccggt gcctcggttg cgaccggcgt
     1261 ctacttcctc cagctgcgcg cgggcagcgc ggtggagacg cgacgattgg tgatgatcaa
     1321 gtagtcgaca tcggtcgagt cggtggatgc ggccggtcgg cgcgaaaatc gccgtccggc
     1381 cgtttccgct cgtgggcgtc cccgcacgcc gcgccctaga ggatgtaacg acgcttgacg
     1441 cgcggatcct ggccggccag cggcccctcg ccgtgcggga agaatcgaag gatgggatga
     1501 aagccgggct ggatcatgcg gatggcctcg cgaaagtcct gattgaccgc ggccagctcg
     1561 cgcttgagcc cctcggtgat gcccgccacg tcgtcggggg cttggctctc ggccgacaac
     1621 tcgatccata tctcgagacg cttgttcgcc gcgtcgtcct cgtagggatg gagcgcgtag
     1681 ttcgccgtca attcctggag cagggggacg cgcgtgattg cgttctggac gtcgtcgggc
     1741 gtgatcttgc acccgtagaa ggcgaccgcg tgctcgcggc ggccccagtg gaacaaaagc
     1801 gggaggttga cgcgcggcgc cggcgggtcc agcgcaacac ggtggtcgcg gagcatctcg
     1861 agtgcgttcc ggcaccgcat cgcgatgccg cggtcgtaga ggttgtaacg gatgcggggg
     1921 gacacgttgt cgaggcgatt gagggtgaac agaaggttgc gctcgccgtc gctttcgatg
     1981 tagtagctca acggatcgta ctgaaagacc atcggcagcc cttcgctccc atacaagtcg
     2041 cgcgccaagt cggcgtttct cgccagctcc tgccgcagcg cgatcgtgaa gtcggtttcc
     2101 accgccatgt tgatttccaa gtcggacgcg ccgaaggagg agatcgttct ttggaagcga
     2161 cggttgagtg ccgcacgcag cccctccgac atgccctcgc cgccgaccgc ggcgtggatg
     2221 cggtacttct tccaatcgat gtccgcgtgg tcgacgaatt gtttcaggaa cggcgggtag
     2281 cccgtgatca agtaccggta gcccggcccc aagtcctgga gtgccgcctc cagcttggcc
     2341 tggtcggggc cgaccgattt caacacgcat tgctcgacga gtgacatcga aacgttcatg
     2401 ccggtcgccc acgggcccag cgcgaacgcg ttgagcagaa tgaagggctc cttgccgaac
     2461 gtgtgccgca cgccgaactg gagcaacctg cgcgtcgcct cacgctcggc gggtccgcgc
     2521 acccagctgg tcgccttgcc gctggagccc gaggactcgt cgatgacggc atcgcgcgac
     2581 ggaaagcgtc cgaactggca gcgcgactcg agcgggtggc ggcggatgta gccgtccttg
     2641 tccatgggcg gcacgtcttc gaagcgccgc gggcgctcgg ccgcgtgctc gcgcaagaag
     2701 tcgcggtacg ccggcacgcg gcggtgcgcg ttccaaaagg ccgcttgcgc gcgcgcgcgc
     2761 gaggcgcgca ggcgcaaacc gctcacgagc ccgggtgcga acaggagcga gtacgccccg
     2821 aacgacgaga gcgccgctgt gtcggcaccc tggagcgcgt tacccagcag cgagtccttg
     2881 atttcattca tggacgatga tcgaggccag ccgcggcgcc cgcggcggta cgggtgagta
     2941 gcccatgcgc aggatgagcc acgcgtcgtt agcgccgaag cggtcggcga cctctcgcgc
     3001 gtaaacgggg ttggtcagca tcgatccgta ggggtgcatg tacacgcggt gacgcgccat
     3061 gatgatccag aactcgagga gcatgcgacc ggcgcggagc agttcgtccc aggtcgaaaa
     3121 gggcccgcac aggagggcta cgtggcgcgt tcccttctgc gtggccagaa atcgccggcg
     3181 cgccaggtga cggacgcccg gcaacttcaa gaaacgcggc gcaaagaacg ccgcccccat
     3241 ttcgagcttg gattggttca tggggagctg ccagagtccg tcgccgtgtt cgggcgtggg
     3301 tccggttcgg taccagccct ggatctccat gcgctcgggc gcgatctgga ggttgtcgat
     3361 gatcgcctcg acgttcgtgt tgagcaccca cgtcaccgtt tcgtcgtcgt cgtaactcga
     3421 gaagcgatgc cccgcgttgc acgcgcacgc gcgcaggtcg gcgagcgcct gcgccgacac
     3481 cggccggccg tcgtagggaa gccgcgacgt tcggcgcgtg tctaacagtt cgggttgatc
     3541 gtgcgcttcg cacgtcccgc gaatagacgc acgcccgagc ctgacgctcg cgcccgggcc
     3601 ctggtggacg gtcgccgggt cgacaccggg ctccgcggtc acgtccacgt ccaggccgag
     3661 gtgacgcccg gaacgaagca gcgcctccac gaacattccg aaggcggcca tgcagtacaa
     3721 gttgccgtgg tcctcgcgcg gcaagaggcg ggacgtcacg cacaccaagt ccgcctcgac
     3781 ctcgctcgtg ggtcgaattc gaaagggctg cgtgttgtgc ggggtcggac agtagcgcgc
     3841 aatccgcgac agtcgattcc aggcgtcgta catcatgaag ccgtagaacc gtcgcgattc
     3901 gactgcgcgc gcacgacgtc gtcgcggacg cactcgagaa agccggtcat catgcggcgc
     3961 aggaacaggc ctcgcacgca cgccatgagc ggatacgcga gcggggacgt gagttggatg
     4021 gtcgcctccc acgtcatccg ggtcccggag ccggtgggag tgaaggtgta gcgcccgcga
     4081 gcggacttcg cgagcaggga aaggggaaag cggtacggcg tgagttgccg gtaggacatc
     4141 gacgaggggg ccgcgatctc gacgatccct tcgttgagca cgcttccgtc ggtcagatgg
     4201 acgcgccgga ccaccccggt tcgcagggca tcgccctcca ggatctctgc gcgcgcgaca
     4261 cccgggagcg gtccgcaccg gcgcacaccg cgcggaagcg tctcgaggtc ggtaaccgcg
     4321 tgccgaaaca ggtcttcggg gggtgccacg cacccaacag tcacagcgac gtgcatgtcc
     4381 atggcggtca accactccaa ccgggcttgg tggaatgcta ccatgcccct cgccctcgcc
     4441 cgcaaccggc atttggcgcg acgtcgcccg gcggccggaa tccgccggca actcccccgg
     4501 ctatttcccg tattcccaga gggtagtcgt cttgaaccgg cacgctgatc ccttggaatg
     4561 gaggcatccg ctcgatgaag aagaaacccg tcctgatagg ctccgcggtc gtggtggtcg
     4621 cggtggtcgt cgtcggagcc ctctcgatgc gaggacgcgg cgagcaggcg atccaggtgc
     4681 agaccgccca ggtgtcgcgg cagaagatcg tgcagaaggt caacggcacc gggaagatcc
     4741 agcccgagac catggtcaag atcagcgccg acgtcagtgc caagatcacg aggctggcgg
     4801 tggtagaagg gcagtgggtc gaaaagggcg cctttcttct ggaactggac cgcgagcgct
     4861 acctggcact agtcgaaagc gcggaagcgg tcgtgcgctc ggcggaagcc gaagcgaagc
     4921 tggtgaagga gaacctcggg cgcgcggaga aggagttcaa ccggtcgaag gagctcgcgg
     4981 gtcgcgacct ggagtcgcag tcgatctacg acgaccgcga ggcagcctac caagtggaac
     5041 tcgcccgcta cgactccgcc aagaatcagg tctcgcaagc ccgagcggcg ctcaaacagg
     5101 ccagggacga cctctccaag accaccatct acgcccccat ggcgggcacc atcagcgacc
     5161 tcatgaagga gcagggcgag atcgcgatcg gctcccagtt ccagcccgac gtcattctcg
     5221 tcattgccga tctctcggtg atggaagccc agatcaacgt cgacgagaac gacatcgtct
     5281 ccgtcgcact cggccaggaa gccgagatcg aggtcgacgc gctgccggac caggtgctca
     5341 agggggtcgt gagcgagatc gcgagcagtg ccaactccac cggcgcgggc tcgaccgatc
     5401 agaaaacgga gttcgaggtc aagatcacgg tgactagccc accgcaaacg ctgcgccccg
     5461 gcatgaccgc gaccgccgac atcttcacca aaaccaacga cgacgcgctc tcggtcccga
     5521 ttcagagcgt ggcggcgcgc acggtcgacc agctcaccgt cgcggggcaa acacgcgagg
     5581 aggccgagaa agtcttcacc accgacaagg acgggttcgt cgagatcgtg ttctgcatcg
     5641 aggaggggaa agcggtcgcg aagcaggtga agacggggat ccagagcgac gacctgatcg
     5701 agatcacgga gggtttgagc gagggcgagg aggtggtgac gggcagctat cgcgccatat
     5761 cgaaggatct caacaacggc ggagccgtca ccgtcgaaaa cgacgagaag ccggacgcca
     5821 aggagaagac ggagcaggcg agtgcgtcca actgacgggc acactccctc gaccaacggg
     5881 gctacgctct ccctgcgcga cgtcgtcaag acctacgtga tgggggccga caccgtgcag
     5941 gcactgcgcg gcgtttcgtt ggacattcaa cgcaacgagt acgtggcggt gatgggcccg
     6001 tccggttccg ggaagtcgac gctcatgaac gtgatcgggt gcctcgacgt ccccacctcg
     6061 gggacctacg cgctcgaggg gcaaatggtg gcgaccatgt ccgagtacca actggccgac
     6121 gttcgcaacc agcgcatcgg attcgtgttc cagaccttca atttgatccc gcgttccaac
     6181 gtctttcaca acgtcgagct gccgctgatc tacgccggcg tggcgaaggc caggcgccgc
     6241 gagcgggtgg agcgcgccat cgagcgcgtg ggactctcca atcgcgtgaa acacaagccc
     6301 aacgagcttt ccggcggcca gcgccagcgc gtggcgatcg cgcgcgcgct ggtggcaaac
     6361 ccgtcgatca tcctggccga cgagcccacg ggcaacctcg acagcaagac cggcgacgag
     6421 atcatgggac tcctggacga gctgcacgcc gccggacaaa ctatcatttt ggtgacccac
     6481 gaggactaca tcgccgagca cgcgcaccgc gtcgttcgcc tgcgtgacgg catgattgaa
     6541 agcgacgagc gagcttcacc ccgcgagggc tgaccatgcg ctggagaatc gacctggaag
     6601 aatccctggc catcgcgctc accgcgttgc gcgcgaacaa ggcgcgtgga gttcttacca
     6661 cactcggcat catcatcggg atcgtcgcgg tgattctcac catgaccgcg gcgcacggac
     6721 tccagagcgc gttccgccag agtttttccg ccgtgggcag cgacgtgatc tacgtgtccc
     6781 gctttccgtg gatcatcatg aacgacttct tcctgtaccg gaatcgcccg cccatggatc
     6841 tgcacgacgc gggggcgctg gccgagcgct tgagcgggaa ggcgatcgtg aatcccacct
     6901 tggggggtca gcaggccgtg cagtatcgcg gcgagaccat ggacgcggtg caaatcatcg
     6961 ggaccacgga aaagcgcacc ctggtggcga gcggtcaagc cgaccgcggg cacttcctca
     7021 ccgatttcga ggtccagtac aagaagaacg tgtgcgtgat cggcaccgag atcgagaagg
     7081 ctctcttcca gggcaccgat ccgctgggaa aggagatcaa gatcgggcgt accgcgttcc
     7141 gcgtggtggg ggtgatggag aagcaggggg gtgggttcct ggggggaccc agcttcgacc
     7201 ggcagatcta cgtgccgatc agctcgtaca tccgcgccta cggaggcgag cgcctggacg
     7261 tgaacctggc cgtaaaggcg ccgtcgcagg aggcactcgg cgacctcgag tacgaagtga
     7321 tcggggaaat gcgcaagatc cgcggacttc gaccgagcga gaaggacgac ttctccatca
     7381 acaagctcga tacgctggtg ggtacgttcg acaacgtcat gggtgtggtg cttctggtgg
     7441 gcctgttgat caccagtatc tcgctgttcg tgggcggcgt tggcgtcatg aacgtgatgt
     7501 tcgtctccgt cacggagcgg acgcgcgaga tcggaatccg caaggccatc ggagccaagc
     7561 cccacagcat tctgttccag ttcttgttcg aatccgccgc gatttgtctg atcggcgggt
     7621 tcatcggcat cgccatcgcg acccttctca ccggcgtgct aaacgcgacc gtcatgcccg
     7681 cgagcgtgtc acccgggatc cttttcaccg cggtggtcgt ggccctggcg gtgggcgcca
     7741 tcgcgggctt cgtccccgcc ttgaagggcg cgcgcatgaa ccccgtcgac gcactgcgct
     7801 acgagtagaa ccgccatgcg cgcctccgat cttctcggca tgtccaccgg agccatcgtc
     7861 ggcagcaaac tgcgctcgtc gctcaccctt cttggaatcg tcgcgggcgt ggcgtcgatc
     7921 atcgccgtga tgaccgcgat tcaggtggtg caatcggcca tggagcacga gatgagcgtg
     7981 ctcggcaccc agaccttcca ggtgcagaag tggccggccg gcttcagctc cgacgaagag
     8041 cgccggaagg cgcgcacccg cccgccgttg accgtcgaca acgccgacgc catccgcgcc
     8101 caggtggcga tggtcgatat cgtcggctcc gaactgtggg acttcggttt caaggccgag
     8161 tacgggggcg aggagaccaa ttccaacctc tcgatcgtgg gcgggacgcc cgagtacccg
     8221 cagaacaaca cccactatgt gggcggcggc cgtaacatct cgcagatcga cgtcaaggcg
     8281 ggccggcgcg tcgcggtcat cggccacgcc atcggcaaga cgctgttccc gtttaccgac
     8341 ccggtgggta aggagctccg actcgacggc cgcaagtacg acatcgtggg tgtgttcgac
     8401 gaaaagaaat ccgcgttcgg atccggatac gacaactacg tgctgatacc cattacgacg
     8461 ttccagtcga tctacggatc gaccacgcgc gacggcttcc cgcgctcggt caacatcacg
     8521 gtgcacgcga aaacaccgga gatgatcgat gacgccatcg aagagacgcg ccaggtgctc
     8581 cggcgcgagc gccgcgtcgc ccccggtgaa cccgacaact tcgacttctt cagcagccag
     8641 agcctcatca cggagttcga caagttgacg gccaagatca agattgcggc gttcgtggtc
     8701 agcatcgtgg ccttgatcgt ggccggcatc gggatcatga atatcatgat ggtctcggtc
     8761 acggaacgta ccaaggagat cggcattcgc aagtcgctcg gtgccaaccc gcgcaacatc
     8821 ctgattcagt ttctcctgga agccgtgatc ttgtgcaaca tcggcgggct ggtggggatc
     8881 gtggtgggct tcgggctggg caacgtaatc acgttcttca cgtccttcga ggtcgaggtg
     8941 cccctgcagt gggccgtcat cggcctggtg ttctgctcca ccgtgggcat cgccttcggg
     9001 ctgcttcccg ccatacgagc ctcgcggctc catcccatcg acgcacttcg ctacgagtag
     9061 ggtctgactc cattagtgcg tgtcaccctc cgcggaatgg ggtaggattg gctagcaggg
     9121 ctgacccatg gcgacccccg acttcgaaaa actcggtgtc ttctacctcg gccgtgagta
     9181 cgatgtcgca cggcgcgcgg cctccgacga tctcgtcctg tacgattcca aggacctgac
     9241 cacccacgcc gtctgcatcg gcatgaccgg cagcgggaag acggggctgt gcattgggct
     9301 tctcgaagaa gccgcgatcg acggtatccc cgccatcgtc atcgatccca agggcgactt
     9361 ggtcgacctc gcgctcgcgt ttccgaatct cgacgcgacg tcgttccggc cctgggtgaa
     9421 cgaggactcg gcgcgccagc agggaatgac tcccgacgag ttcgccgcgc gcgaggccga
     9481 caaatgggcc aagggactcg cgcagtgggg gcaggacgcc gcgcgcatcc gccgcctgcg
     9541 cgacgcggcc gacgtcgtcg tgtacacccc cggcagcgac gccgccatcc ccgtgtccgt
     9601 gctctcgtcg ctcgagtgcc cgaagggcga cgcgtccgcg gacctgttgg cggaacgcgc
     9661 cagcggcgtg gcctcgagcc tgctctcgct cctgggcatc gaggccgacc cggttcgcag
     9721 ccgcgagcac atcctcctca cgacgattct tcagagcgcg tggtcgcaag cgcgaagtct
     9781 cgacctcgcc ggtctggtgc aggcggtgca gtcaccgccg gtcgcgcgcg tgggtgtgat
     9841 ggagctcgag tcgttcttcc cggccaagga gcgcttcgcg ctcgcgatgc tcgtcaacaa
     9901 cttgctcgcc gcgccgcgct ttcaatcgtt cctcgccggc gatcccatcg acatcgatcg
     9961 catgctctac ggcgaaacgg gaaaaccgcg cgtggccgtg ttttccatcg cgcacctgga
    10021 cgacgcggag cgcatgttct tcgtctcgct cctgctgaac gaagtggtgg gctggacgcg
    10081 gacacgctcg gggacctcg
//
LOCUS       DASPGK010000306         7974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_313311, whole genome shotgun sequence.
ACCESSION   DASPGK010000306 DASPGK010000000
VERSION     DASPGK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313311"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VEC56_08570"
     CDS             <1..978
                     /locus_tag="VEC56_08570"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HXV14248.1"
                     /translation="GSVLRGRDSVLVANPLEDPRFGGQQSIMALSLRSAMAAPLFDNE
                     RVRGILYVDTTDPRVRYTQADLEVLTAAANAVAVKLRNLSLEEEIGTAARIQRSMLPD
                     AVRVPEGYEVDAHQVMCRAVGGDLYFVGARPDGSVLMALGDVTGKGMPAALAMSAATV
                     AIGLLADIEGDVKMLAGRLHRQLFKSLAEEQFITLFIAELDPGTGRLRYVNAGHEPPL
                     VVRRDGTLELLESTTLPMAMIESIPLDAKETTLAPGDSVAVFSDGIPEATTDGENFLG
                     LDGVKDALVTHRAESLPALRERVVALVERFLAGGRASDDVTLLMLRRAA"
     gene            complement(981..1454)
                     /locus_tag="VEC56_08575"
     CDS             complement(981..1454)
                     /locus_tag="VEC56_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14249.1"
                     /translation="MIRKLLFVVLAAVLAVSAVAPASAQEKRVGLGFIVGEPTGIDLK
                     FFLNNTNALEFALAWSLSDENDLHLQGDYLWHRFDVIDLNNGDDMPLFFGIGGRVIFR
                     EDPIDDHVGIRFPVGLNYIFADYPFDIFAEIVPILDVAPDTDFDLEGAIGARFWF"
     gene            1631..2344
                     /locus_tag="VEC56_08580"
     CDS             1631..2344
                     /locus_tag="VEC56_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HXV14250.1"
                     /translation="MFFDPLYLMVIGVGMALSIWASLKTKGTFHKFSQYTTNARMTGA
                     DVAKAILRDANIHDVTVEPVRGTLTDHYDPRTKTLRLSEAVYGSASMSAAGVAAHEVG
                     HAIQHAQAYGPLKFRSAWVPVANLGGGISIFVLIAAAMLGGAATATGHNLAILGVVLF
                     SCTTVFTLVTLPVEFDASKRALATLRRGSYLTPDELAGAKKVLDAAALTYVAAFVTSA
                     LTLLYWAMRLGLLGGRRND"
     gene            2473..2892
                     /locus_tag="VEC56_08585"
     CDS             2473..2892
                     /locus_tag="VEC56_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14251.1"
                     /translation="MHLRLVPHLAMLLASASIGCSDLGDPYQPKPADSASISFAGDVQ
                     PIFNAHCVLCHGTLVPPPNNLDLRPGNSYANLVGRTSVGYAPNLRVSPGDTLASVLYG
                     KVTGTNFGEQMPPGFLLIPRPERDRIVAWIAEGAPNN"
     gene            2889..4007
                     /locus_tag="VEC56_08590"
     CDS             2889..4007
                     /locus_tag="VEC56_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14252.1"
                     /translation="MNARARAGGSTVAIALVLLAGAAGPAGAVPRYSARYEQKCALCH
                     VNPSGGGMRTLYASQYLVPEEMAWSKPDSEILASIDPEIAKRIQIGTDFRTLYTYSNE
                     SADAAVSDFFQMQGDVYLNFQMDDVLSLYYDQGISSSYELFGLWQGLPLTGYVKVGRF
                     VPAYGWRFDDHTMYTREALGFSPPTNSDVGVEFGLSPGRLDMQLSVVNGNRGGTFDDN
                     QKLAAVLNAAYRGRVGPLGWAGGVAGYWEDGDAANFGSGGPYGYLTWNRLTWLGEVDW
                     FLEDPQGTGHTTGLVASNELSYLVRQGLELKATYDFLDPDWNFETGAKRRAGGGVFVL
                     AKPFVAVEALYRVTRYDDGVALSGEDDWETVLQLHLLY"
     gene            4020..4604
                     /locus_tag="VEC56_08595"
     CDS             4020..4604
                     /locus_tag="VEC56_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXV14253.1"
                     /translation="MKRSGWFVVAAFLIPAPASAEEFVVKAGAPNKVVFVSKAATETF
                     DGKTDRITGRIVVDPAGVIDTVTVNLEVDMASLDTGIGKRNSHMRENHLETDKYPKAV
                     FAGASVLGPEGATLAPGKPVSFECEGDFTLHGVTKRLRVTVEVTQRDARTLAFKTSFK
                     VPLADYQIDRPKFLFLKLGEVQDVTVEGVAIAAP"
     gene            complement(4612..5307)
                     /locus_tag="VEC56_08600"
     CDS             complement(4612..5307)
                     /locus_tag="VEC56_08600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14254.1"
                     /translation="MISRIRRSLGTHGFVATMRLARRRLSGAFARLVDRHFDWAFGTD
                     TRTMIENADLRDVESSNLARGIRYEPTLAIPFRRALRAARVPSGGTFVDIGCGKGRAC
                     MLAAMHGFATVSGVDYSPSLCRIAEQNLEIFRARTNRHFRSSIRAMDAADYAFAAGDT
                     VVYLFNPFDAGVLSAVVARLRRSLSTHPREVWIVYQNPRWRGVIEAGGDFVSVGSWTY
                     GGCDFAVYRSRVA"
     gene            5535..6500
                     /locus_tag="VEC56_08605"
     CDS             5535..6500
                     /locus_tag="VEC56_08605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV14255.1"
                     /translation="MRLLPENERVRERYAELVAHCETATVYQTTAWLDVWKHLGADVA
                     FVEVDDATMVPFVCRGRGVLRRAYSLPFDTYGGPVTAHPNGPVSFERAVEPLGNASAR
                     VVDFGARMASSNGAARRVTSHVVDLSRGYEAAAQHYSDSNHRLIRQARDHGVRIDVVH
                     DASQVRAFYAMHLRTVARYGARPLPRAFFDAVFASLVPAGLATFYLARHDGAAVAGNL
                     VLRRRDRSFDWMWVYDDRHLPLRATNLMIDITIRDEAARGARLLNLGASPNDRLGSVR
                     FKQGFGAEPFGYTVYAHTGRLVAAARRARENTNRFGARLRVLAVR"
     gene            complement(6544..7116)
                     /locus_tag="VEC56_08610"
     CDS             complement(6544..7116)
                     /locus_tag="VEC56_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV14256.1"
                     /translation="MSASPLPSKVAALRDELNDATSRLHRLLDTVDESTWGRSPAPGK
                     WSAARCVEHLNLTSRAYLPLFRAAFKDARARGLVAKNPTYELDFWGWLLIKSFESRLR
                     MKTPDSFVPPTIEPKDKVLREYDDLQNELSALLVENGDLALGKIKIVSPFNASITYHV
                     YSAYRIIPSHQRRHLAQAERVLMTLATQSR"
     gene            complement(7281..>7974)
                     /locus_tag="VEC56_08615"
     CDS             complement(7281..>7974)
                     /locus_tag="VEC56_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14257.1"
                     /translation="VGAEDLGPGTYTYYPAVMVDCELNMALGFSASNAAIFAGAYYAT
                     RRAADAPGFIAPTDGLKPGVAPYKRFFSGTRNRWGDYSGLAICPNGEADFWVFNEYAG
                     PVGTPTVGSMGPEDGRWFTAVGTFRIKAPTAAETPPTTTRLAQNIPNPFNPATRIQFS
                     LAAREHVTLAVYDATGRKVRILVDETRDAGTHEAVWDGRDGRSKARASGVYFYRLTAG
                     NYSETKKMLLLK"
BASE COUNT         1344 a         2631 c         2651 g         1348 t
ORIGIN      
        1 gggagcgtgt tgcgcggacg cgactccgtg ctggtcgcga acccgctcga agacccccgc
       61 ttcggcggcc agcagagcat catggcgctg agcctgcgct ctgcgatggc cgcaccgctg
      121 ttcgacaacg agcgcgtgcg cggcatcctc tacgtcgata ccaccgaccc gcgcgtccgc
      181 tacacccagg ccgatctcga ggtgctcacc gcggccgcca acgcggtcgc ggtcaaactg
      241 cgcaacttga gcctggagga ggaaatcggg accgcggcgc gcatccagcg ctccatgctc
      301 cccgacgcgg tgcgcgtccc cgagggctac gaggtcgacg cgcaccaggt catgtgccgc
      361 gcggtgggcg gcgacctcta cttcgtgggt gcgcggccgg acgggagcgt gctgatggcc
      421 ttgggcgacg tcaccggcaa gggaatgccg gccgcacttg cgatgagtgc cgccacggtg
      481 gccatcggat tgctcgccga catcgagggc gacgtgaaga tgctcgccgg gcgcctgcat
      541 cgacagctct tcaagagcct ggccgaagag cagttcatca cgctgttcat cgccgagctc
      601 gaccccggca ccgggcgctt gcgctacgtc aacgcgggcc acgagccgcc gctggtcgtg
      661 cgacgcgacg gcacgctcga gttgctcgag tcaaccacgc tccctatggc gatgatcgaa
      721 agcatcccgc tcgatgcgaa ggaaaccacg ctcgcgccgg gcgattcagt cgcggtgttc
      781 agcgacggca tccccgaagc aactaccgac ggcgagaact tcctcggcct cgacggagtc
      841 aaggatgcgc tcgttacgca ccgggcggaa tcgctgcctg cattgcgcga gcgagtcgtg
      901 gcgctggtgg agcggttcct cgccggcggg cgcgcgtcgg acgacgtcac gctgctcatg
      961 ctccggcgcg ccgcctaggg ttagaaccag aagcgcgcgc cgatcgcacc ttccaggtcg
     1021 aagtcggtgt cgggcgccac gtccagaatg ggcacgatct cggcgaagat gtcgaacggg
     1081 taatccgcga agatgtagtt gagtccgacg gggaagcgga taccgacgtg gtcgtcgatg
     1141 ggatcctccc gaaagatcac tctaccgccg atcccgaaga acaacggcat atcgtcgccg
     1201 ttgttcaggt cgatgacgtc gaagcggtgc cagaggtaat cgccctgcag gtggaggtcg
     1261 ttctcgtccg agagcgacca cgcgagcgcg aactcgaggg cgttggtgtt gttgaggaag
     1321 aacttgaggt cgattccggt gggctccccg acgatgaagc cgagacccac ccgcttctcc
     1381 tgggccgaag ccggggcgac ggccgagacc gcgagcaccg cggccaggac gacgaataag
     1441 agctttcgaa tcatgaggtc tcctcgactg gaacaggtcg gttgaaacag ctatattagc
     1501 ccggtgttca attagccggc gccgggggcg ccgatggccg ggatgatacg catccgagca
     1561 ccggccgagc aacccccgaa tcccaataac tgtaataatc gccactgtgg tttctgtcgg
     1621 aggatgacga atgtttttcg atccgttgta tttgatggtg atcggtgtcg gcatggcgtt
     1681 gagcatctgg gccagtctca agaccaaggg gacgttccac aagttcagcc agtacacgac
     1741 caacgcgcgc atgacgggcg ccgacgtggc caaggccatc ctgcgtgacg cgaacatcca
     1801 cgacgtcacc gtcgaaccgg tgcgcggaac gctcaccgac cattacgacc cgcgcaccaa
     1861 aacacttcga ttgagcgaag ccgtgtacgg aagcgccagc atgtcggccg ccggtgtcgc
     1921 tgcgcacgaa gttggccacg ccatccagca cgctcaggcg tacggtccgc tcaagttccg
     1981 ttcggcgtgg gttcccgtgg cgaatctggg cggcggcatc tcgatcttcg tgctgatcgc
     2041 ggccgccatg ctcggcggcg ccgcgaccgc gaccggccac aacctcgcga tcctgggcgt
     2101 ggttctgttc tcgtgcacca ccgtgttcac gctggtaacg cttccagtcg agttcgatgc
     2161 gagcaagcgc gcgctcgcca ctctccggcg cggcagctac ctgacacccg acgagctcgc
     2221 gggcgcgaag aaagttctgg atgccgcggc gctcacgtat gtggccgcct tcgtcacctc
     2281 ggcgctcact cttttgtatt gggcgatgcg gctcgggctc ttgggtggtc gtcgcaacga
     2341 ctgattcggt cgacatcccc tttgtttggc ccagggcccc cgcgatcacc gtcgcggggg
     2401 ccctttttgc aatccccgcc tttccaaatc cgagacgacg tgttaacctg gaccgacccc
     2461 tgctgacaac ctatgcacct caggcttgtc ccgcacctcg cgatgctact cgctagcgcg
     2521 tcgatcggtt gttccgacct cggcgacccc taccagccca agccggccga cagcgcgtcg
     2581 atatcgtttg cgggagacgt gcagccgatc ttcaacgcgc attgcgtcct ttgccacggg
     2641 acgctcgtgc cgccgccgaa caacctcgac ttgcggccgg ggaacagcta cgcgaatctc
     2701 gtcgggcgca ccagcgtagg ctacgcgccc aacctgcgcg tgagtcccgg cgacacgctg
     2761 gcgtcggtgc tgtacggcaa ggtcaccggc accaacttcg gcgagcaaat gccgcccgga
     2821 ttcctgttga ttccacgacc ggaacgcgat cgtatcgtgg cgtggatcgc ggaaggagcg
     2881 cccaacaatt gaacgcgcgc gcgcgggcag gaggatcgac cgtcgcgatc gcgttggtct
     2941 tgctggcggg tgcggccggg cccgcgggcg cggtgccgcg ctactccgca cgctacgagc
     3001 agaaatgcgc actttgccat gttaatccgt cgggcggagg catgcgcacg ctgtacgcgt
     3061 cgcaatactt ggtgccggaa gaaatggcgt ggtcgaagcc cgactcggag atcctggcga
     3121 gcatcgaccc cgagatcgcg aagcgcatcc agatcgggac ggatttccgc accttgtaca
     3181 cgtactcgaa cgagagtgcg gatgccgccg tcagcgactt tttccagatg cagggcgacg
     3241 tctacctcaa cttccagatg gacgacgttc tttcgctcta ctacgaccag ggcatctcgt
     3301 cgagctacga gctgttcggg ctgtggcagg ggctcccgct caccggctac gtcaaggtgg
     3361 ggcgcttcgt gcccgcgtac ggctggcgct tcgacgatca caccatgtac acgcgcgagg
     3421 cgctcggctt ctcaccgccg accaacagcg acgtcggcgt ggagttcgga ctttcgccag
     3481 ggcgcctgga catgcagctt tcggtggtca acggcaatcg cggcgggacg ttcgacgaca
     3541 accagaagct cgccgccgtg ctcaacgccg cgtaccgcgg ccgcgtcggc ccgctcgggt
     3601 gggcgggggg agtcgcgggg tactgggagg acggcgacgc cgccaacttc ggcagcggtg
     3661 gcccatacgg gtacctcacg tggaatcggt tgacgtggct cggcgaagtg gattggtttc
     3721 tcgaggatcc acagggcacg ggacacacga ccggcttggt cgcgtccaac gagttgtcgt
     3781 atctcgtgcg gcagggtctc gagctcaagg ccacgtatga ttttctcgac ccggactgga
     3841 atttcgagac gggggcgaag cggcgcgcgg gcggcggtgt gttcgtattg gcgaagccgt
     3901 tcgtcgcggt cgaagcgctc tatcgcgtta cgcgctacga cgacggcgtg gcactctcgg
     3961 gcgaagacga ctgggaaacg gttctgcaac ttcacctctt gtactagcgg ggtatcgtca
     4021 tgaagcgttc aggttggttc gtggtggccg cgttcttgat accggcgccc gcgagtgccg
     4081 aggagttcgt cgtcaaggcg ggtgcgccca acaaggtggt ctttgtctcc aaggcggcga
     4141 ccgagacctt cgacggcaag accgatcgca tcacggggcg catcgtagtc gacccggccg
     4201 gcgtgatcga caccgtcacc gtgaatctcg aggtcgacat ggccagcctc gacaccggta
     4261 tcggaaagcg caactcgcac atgcgggaga atcacctgga gaccgacaag tatccgaagg
     4321 cggtgttcgc gggcgcgtcg gtgctggggc cggaaggcgc gaccctggcg ccgggcaagc
     4381 cggtgtcgtt cgaatgcgag ggcgacttca cgctccacgg cgtcaccaag cgcctgcgcg
     4441 tgacagtcga ggtgacgcag cgtgacgcgc gcacgctcgc gttcaagacg tcgttcaagg
     4501 tgccgctcgc cgactaccag atcgaccgcc cgaagttcct gttcctgaag ctgggcgaag
     4561 tgcaagacgt gaccgtcgag ggcgtcgcga tcgccgcacc ctaggaccgg ctcaagcgac
     4621 gcggcttcga taaaccgcga agtcgcagcc gccgtaggtc caacttccga cggacacgaa
     4681 gtccccgccc gcctcgatca cgccgcgcca gcgcggattc tgatacacga tccacacctc
     4741 gcgcgggtgc gtcgacagcg accgccgcaa ccgcgccacc accgcgctca acacccccgc
     4801 atcgaacgga ttgaacaggt acacgacggt gtcgccggcc gcgaaggcgt agtccgccgc
     4861 gtccatggct cgaatgctgg agcggaaatg gcggttggtg cgggcgcgga agatctccag
     4921 gttctgctcg gcgatgcggc acaggctggg tgagtagtcg acgccgctca ccgtggcgaa
     4981 gccgtgcatc gcggccagca tgcaagcgcg ccccttgccg cacccgatgt cgacgaacgt
     5041 ccccccgctc ggcacgcgcg cggcgcgcag cgcgcggcga aaggggatcg cgagagtggg
     5101 ctcgtagcgg atgccgcgcg cgaggttgct gctctcgacg tcgcgcaagt ccgcgttctc
     5161 gatcatcgtg cgcgtgtcgg tgccgaaggc ccagtcgaag tgccggtcga ccagcctcgc
     5221 gaacgcgccc gagaggcgtc gtctcgccag tcgcatggtc gcgacgaaac cgtgcgtgcc
     5281 gagactcctt cggattcgag aaatcatgcg tcgtggttcg ttcgagagcg ttcgatcgcg
     5341 gcgtcggcat gcgaagtcgg gggttgacag cgcgccccgc atcggtcatc atatgtccga
     5401 gccgccccac actatacgcg gccacccctc gccgggacaa cgatccccaa ggagttcgct
     5461 ccgtccttct gctaccgccc gccggtgttc aaccgccggc accatcgttt cgaaataacc
     5521 aaagcgggga ccctttgcgc cttcttcccg aaaacgagcg cgttcgcgag cgctacgcgg
     5581 agctcgtcgc gcactgcgag acggccaccg tctatcaaac cacggcctgg ctcgacgtgt
     5641 ggaagcacct gggcgccgac gtcgccttcg tcgaagtcga cgacgcgacc atggtgccgt
     5701 tcgtgtgccg cggccgcggt gtgctccggc gcgcgtactc gctgccgttc gacacctacg
     5761 gcggcccggt gaccgcccat cccaacgggc cggtgtcgtt cgagcgcgcg gtcgagccgc
     5821 tggggaacgc gtcggcgcgc gtagtcgatt tcggcgcacg catggcgtcg tcgaacggcg
     5881 ccgcgcgccg ggtgacgtca cacgtggtcg atctgtccag gggatacgaa gccgccgcgc
     5941 agcactacag cgattcgaac caccgcctga tccggcaggc gcgcgaccac ggcgtgcgca
     6001 tcgacgtcgt gcatgacgca tcccaggtgc gcgcgttcta cgcgatgcac ctgcgcacgg
     6061 tggcgcgcta cggtgcgcgc ccgcttccgc gcgcgttctt cgacgcggtg ttcgcctcgc
     6121 tcgtgcccgc gggcctcgcg acgttctacc tcgcgcgcca cgacggtgcc gccgtggccg
     6181 gcaacctcgt gctgcgccgg cgcgaccgct cgttcgactg gatgtgggtc tatgacgacc
     6241 ggcaccttcc gctgcgcgcg accaacctca tgatcgacat caccattcgc gacgaagccg
     6301 cgcgcggtgc gcggctcctg aacctgggcg cgagccccaa cgaccgattg gggagcgttc
     6361 gcttcaagca gggtttcggc gccgagccct tcgggtacac ggtctacgcg cacaccggcc
     6421 gcctggtcgc ggccgcgcgt cgcgcccgtg agaacaccaa ccgattcggc gcgcgcctcc
     6481 gcgtcctagc agttagatag cagcacttgc ttcacgaccc ggacacgggg cgcgcgcctc
     6541 ttctcaccgc gattgcgtcg ccaacgtcat gagcacgcgc tccgcttgcg ccagatggcg
     6601 gcgctggtgg gatggaatga tgcggtatgc cgagtacacg tggtacgtga tactggcgtt
     6661 gaagggggag acgatcttga tcttgccaag cgcgaggtcg ccgttttcca cgagcagcgc
     6721 gctcagctcg ttttgcaggt cgtcgtactc gcgcaggacc ttgtccttgg gttcgatggt
     6781 gggtgggacg aaggaatccg gcgtcttcat gcgaagacgc gactcgaacg acttgatcag
     6841 cagccagccc cagaagtcga gctcgtacgt cggatttttc gccaccagac cgcgtgcgcg
     6901 cgcgtccttg aaggcggcgc ggaagagcgg gaggtaggcg cgcgaggtga gattcaagtg
     6961 ctcgacgcaa cgcgccgccg accacttccc cggagcgggg ctgcggcccc acgtgctctc
     7021 gtcgacggtg tcgaggaggc ggtgcagccg cgacgtcgcg tcgttcaatt cgtcgcggag
     7081 ggccgcgacc ttcgatggaa gcggtgaggc gctcacccag actccttccc aggccgagcg
     7141 cggatgatac gaccgcgccg gtcgcgggtc aaaggaagtc gagcgcggtg cggatcgggc
     7201 ggcgaggcgc cacggtctct ccgcgcgtcc ccgccggcgg cggcggggag cgcgcagaaa
     7261 ccgagcgtcg ccgaaaggac ctacttcaac aatagcatct tcttcgtttc gctatagttg
     7321 cccgcggtca ggcgatagaa atacacgccg gaggcccgcg ctttcgagcg tccgtcgcgc
     7381 ccgtcccaga cggcctcgtg ggttccggca tcgcgcgtct catcgacgag tatgcgcacc
     7441 ttccggcccg tcgcgtcata caccgcgagc gtcacgtgct cgcgcgcggc gagggagaac
     7501 tggatccgcg tcgccgggtt gaacggattg ggaatgttct gcgcgagacg ggtcgttgtc
     7561 ggcggtgtct cggccgccgt gggtgccttg atgcggaagg tccccacggc cgtgaaccag
     7621 cggccatcct cgggacccat gctgccgacc gtcggcgtcc ccaccggacc ggcgtactcg
     7681 ttgaagaccc agaagtccgc ctcgccgttg gggcagatgg cgagaccgct gtagtccccc
     7741 caccgattcc gcgtcccact gaagaaacgc ttgtagggcg cgactcccgg tttgaggccg
     7801 tcggtaggcg cgatgaagcc gggggcatcg gcggccctgc gcgtcgcgta atacgcgccc
     7861 gcgaagatgg cggcattcga cgcggagaaa ccgagggcca tgttgagctc gcagtccacc
     7921 atcaccgccg gatagtaggt gtacgtgccc ggtcccaagt cttcggcccc gacg
//
LOCUS       DASPGK010000307         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_897971, whole genome shotgun sequence.
ACCESSION   DASPGK010000307 DASPGK010000000
VERSION     DASPGK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897971"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VEC56_08620"
     CDS             complement(<1..700)
                     /locus_tag="VEC56_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXV14258.1"
                     /translation="MERFELQSNVRADAGKGVARKLRGEGRLPAVLYGRKEAPMGISL
                     AEVDVRNILRKHPESAIVDLTVAGEGGGTLNAIIRDVQRHPASGKILHVDLQRIRLDE
                     QIRVEVHIEVQGTPAGVKEQGGVLEHGTRTVNVMTLPTDIPDAILVDVSALRIHDSAK
                     IRDVMANYPKIVFLDDPDTTLATVIPPIVEAVVAPTAEVTAEPELIRKPGAEEEGEEG
                     AAAAPAKGAAPAKSA"
     gene            complement(716..1648)
                     /locus_tag="VEC56_08625"
     CDS             complement(716..1648)
                     /locus_tag="VEC56_08625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXV14259.1"
                     /translation="MDGLKLISGNANRPLAEKVGEQLGVPLCDVTLDRFADGEIQCKI
                     NENIRGVDVFIVQSTQPPAENLMELLILLDACFLASAERITVVTPYYGYARQDRKDQP
                     RVPITAKLIAKLLEVAGTNRILTLDLHSTQIQGFFDIQADNLFAAPVILEYIKQLDTS
                     NLMIVSPDVGSTKIGRAFAKRLDVDLAIVDKRRSEKDNVEVMNVIGDVKGRDVVILDD
                     MITTGGTLVKAARAVSDKGARSVLACATHPVFAGDACKIIMNSPIREVVVTNSLPFDR
                     FAECPKVKVLDISRLIAEAIKRIHYNESVSTLFV"
     gene            complement(1727..1801)
                     /locus_tag="VEC56_08630"
     tRNA            complement(1727..1801)
                     /locus_tag="VEC56_08630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1767..1769),aa:Gln,seq:ttg)
     gene            complement(1854..2147)
                     /gene="spoVG"
                     /locus_tag="VEC56_08635"
     CDS             complement(1854..2147)
                     /gene="spoVG"
                     /locus_tag="VEC56_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HXV14260.1"
                     /translation="MRITEVRVSLRDDAKLKAFASITLDDCFVIRGLKIIEGKDGTFV
                     AMPSRRRKDGEYQDIAHPINNDAREEMERVILAEYRKEKERAKVAETPHMSDR"
     gene            complement(2210..3106)
                     /gene="ispE"
                     /locus_tag="VEC56_08640"
     CDS             complement(2210..3106)
                     /gene="ispE"
                     /locus_tag="VEC56_08640"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXV14261.1"
                     /translation="MAVRVSALAPLDVVTRAKVNLSLEVIRRRPDGFHEIETILQSID
                     LADRMRVELTDDAKVEIECATPGIPTDASNLCHRAILAMRKVAGRSLGARIVLEKHIP
                     VGAGLGGGSSNAAGIVLAVDRALELDLPRKTLEKIAASLGSDVPFMLRGGTMFGRGRG
                     EILTPLDTLKGAFFVVVKPEVSIPTAWVYSNLSFRLTGHRPRLNLRAVSAVLARFPKA
                     PLPFRNVLEEVVFPAYPSVAKLWEELISERPCFASMTGSGSAVFAIFDRESRAVAVAE
                     RFSVRGLFASVAKPAKQAVDIR"
     gene            complement(3182..4069)
                     /locus_tag="VEC56_08645"
     CDS             complement(3182..4069)
                     /locus_tag="VEC56_08645"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HXV14262.1"
                     /translation="MKQVYYIGASMRPKQWTKNLIVFAGLIFSRHFRDVDLLVRSLEA
                     FVVFCILSGAIYLVNDVADVDKDRLHPVKRARPLASGAMKRSTALGAAALMIVAALAW
                     SYRLGPGFLVVACVFTALNLVYSFGLKRVVLLDVASISLSFVLRAIAGVEALRGVDPT
                     IAISPWLLICTLFLSLFLAFCKRRHELVNLEDAVNHRESLQEYSPALLDQLVGISASG
                     SVLSYSIYTIWPETVEKFGTANLVYTVPLVLIGIMRYLYLVYKKEKGGSPSDLLLHEK
                     FLLVDVLAWIVLVVVILGI"
     gene            complement(4066..>5256)
                     /locus_tag="VEC56_08650"
     CDS             complement(4066..>5256)
                     /locus_tag="VEC56_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14263.1"
                     /translation="HGVPVEGPAERLGDACARHRVDALAYGLPGEDDVTAERWLAFAR
                     RANLSIERLPGSAGAREPASLALDRVARRYRRGVIDPSPRSQAALRGARVLVTHGGER
                     IGGALIARLRAVGAVPVLHVDDPRAVSEGALRDVVHYAGPLPVAAVDAIAATRPDVVV
                     HAVRVEPSGAENEDERAWHHVVRESDLLARELWTQRPGCRLVVAAFWGGARPGDRAAA
                     VAAAMEAAVLNRAGAEPASVVRLPRILTAALLADTSWTKEPPSHTRFDALESEAAALL
                     AEIAGGAFRGIYTLAPAAPIDLDDAGRAAARGDDESDRDPAPAAPGAGLVFPSEHLDL
                     CGIDGARRVLSPLFPAADPFRKLATHSPADATRAEREAWTRAVTAQLYHVGRASEAAP
                     RSET"
BASE COUNT          912 a         1758 c         1721 g          865 t
ORIGIN      
        1 cggccgactt cgcgggtgct gcccccttcg cgggagccgc ggccgcgcct tcctcgccct
       61 cttcctcagc acccggctta cggatcagct cgggctcggc cgtgacttcg gccgtgggag
      121 caaccaccgc ttccacgatg ggcgggatca cggtcgcgag ggtggtgtcg gggtcgtcga
      181 gaaacacgat cttcggataa ttcgccatca cgtcgcggat cttggccgag tcgtggatgc
      241 gcagcgccga cacgtcgacc aggatggcgt cggggatgtc ggtgggcagc gtcatcacgt
      301 tgacggtgcg ggtgccgtgc tcgagcacac cgccctgctc cttcacaccg gccggtgtgc
      361 cctgcacctc gatgtgtacc tcgacgcgaa tctgctcgtc caagcgaatg cgctgcaggt
      421 cgacgtgcag gatcttgccc gacgcggggt ggcgctggac gtcacgaatg atcgcgttca
      481 gggtcccgcc gccctcgccc gcgaccgtca ggtcgacgat ggcgctttcg gggtgcttgc
      541 gcaggatgtt gcgcacgtcc acctcggcga gcgagatgcc catgggcgct tccttgcgac
      601 cgtacaacac ggcgggcagg cggccttcgc cgcgcaactt gcgcgcgaca cccttgccgg
      661 cgtcggcgcg aacattcgac tggagctcaa atcgttccat gatccttatc ctccgctaca
      721 cgaacaacgt actgaccgac tcgttgtaat gaatcctctt gatggcttcc gcgatgagtc
      781 gcgagatatc gagcaccttc accttggggc actcggcgaa gcggtcgaac ggaagcgagt
      841 tcgtcacgac cacctcgcga atgggcgaat tcatgatgat cttgcacgcg tcgccggcga
      901 acaccgggtg cgtcgcgcac gccagcaccg aacgcgcgcc cttgtcggag accgcgcgcg
      961 ccgctttgac cagggtaccg ccggtggtga tcatgtcgtc tagaatcacc acgtcgcgcc
     1021 ccttgacgtc gccgatcacg ttcatgacct cgacgttgtc cttttcggag cggcgcttgt
     1081 cgacgatggc caggtcgacg tccaaacgct tggcgaacgc gcggccgatc ttggtgctgc
     1141 ccacgtcggg cgacacgatc atcaagttgc tggtatcgag ctgcttgatg tactccagga
     1201 tcaccggcgc cgcaaacagg ttgtcggcct ggatgtcgaa gaacccctgg atctgcgtgc
     1261 tgtgcaggtc caaggtgaga atgcgattgg tccccgccac ctccaggagc ttcgcgatca
     1321 acttggccgt gatgggaacg cgcggctggt ccttgcggtc ctggcgcgcg tacccgtagt
     1381 acggtgtgac cacggtgatc cgttccgccg acgccaggaa gcacgcgtcg aggaggatca
     1441 acaactccat gagattctcg gccggcggtt gcgtcgactg cacgatgaac acgtcgacgc
     1501 cgcggatgtt ctcgttgatc ttgcactgga tctcgccgtc ggcgaatcgg tcgagggtca
     1561 cgtcgcacaa gggaacgccc agctgctcgc cgactttttc ggcaagaggc cggttggcgt
     1621 taccggagat gagcttcaga ccgtccatgg tgctttctcg ggcaatcgat tgcgagtgac
     1681 cgttttcgtt tcgtgccgcg cgacgggact atccggcgcc tgcccgtggt tggggcggga
     1741 ggattcgaac ctccgaatgc ttggtccaaa gccaagtgac ttgccgcttg tctacgcccc
     1801 aggaaaacta aagaagaagg actgctcctt cgaggagcgc cggggacggc gcttcagcgg
     1861 tcggacatgt gcggagtctc cgctaccttc gctcgttcct tttccttgcg gtattcggca
     1921 agaatgacgc gctccatctc ttcgcgcgca tcgttattga tcggatgcgc gatgtcctgg
     1981 tactcgccgt ccttgcggcg gcggctgggc atggcgacga acgttccgtc tttgccctcg
     2041 atgatcttca agccacgaat gacgaagcaa tcgtcgagcg tgatgctggc aaacgccttg
     2101 agcttcgcgt cgtcgcgcag agataccctc acttcggtga tcctcatcga gcccccctat
     2161 ccgtaacaag acaagcagac acccacactg ggcgcgcgaa gcgcgcaccc taacgaatgt
     2221 cgaccgcttg cttcgctggt ttcgcgacgg aggcgaacaa ccccctcacc gagaatcgct
     2281 cagctaccgc aacggcccgc gactcgcgat caaagatggc gaacaccgcg gatccactgc
     2341 cggtcatgga cgcaaagcaa ggccgctcgg atatcaactc ttcccacagc ttcgcgaccg
     2401 atggataggc cggaaatacc acttcttcca gcacattccg aaacggcaac ggagccttcg
     2461 gaaaccttgc caaaacggca cttacggccc tcaggttaag tcgtggtcgg tgccctgtca
     2521 atcgaaaact aaggttggaa taaacccagg ccgtggggat gcttacctcc ggcttgacga
     2581 cgacgaaaaa ggctcctttg agggtgtcca agggggtgag aatctcgccc cggccccgcc
     2641 cgaacatggt cccgccgcgc agcatgaagg gcacatcgct gcccagcgat gcggcgattt
     2701 tctccagagt cttcctcggc aaatcgagtt ccagcgcgcg gtcgaccgcg agcacgatgc
     2761 cggctgcatt cgagctgccc ccccccaatc cggccccaac cgggatgtgt ttttccaata
     2821 cgatgcgggc tcccagcgac cggcccgcga ccttgcgcat ggcgaggatc gcgcggtggc
     2881 ataggttgct cgcgtcggtg ggaatgccgg gagttgcgca ttcgatctcg accttggcgt
     2941 cgtccgtcaa ctcgacgcgc atgcggtccg cgaggtcgat cgactgcagg atggtctcga
     3001 tctcgtggaa accgtccggg cgccggcgga tcacttcgag cgagaggttg accttcgcgc
     3061 gcgtgaccac gtccagcggt gccagagcgg acacgcgcac cgccacgcgg tcgcgcaccc
     3121 gatgcacgcg cgcgggtccg gtcggaccgc gacgggccgg agtcgctcgt ctcgtcacca
     3181 tctagatgcc caggatcacg acgacgagca cgatccacgc gaggacgtcg accaggagga
     3241 acttctcgtg caggaggaga tcggacgggc tgccgccctt ctctttctta tagaccagat
     3301 agagataccg catgatgccg atcaacacca gcggcacggt ataaacgagg ttggcggtcc
     3361 cgaacttctc caccgtctcc ggccaaatgg tatagatcga atacgagagc accgagccgc
     3421 tggcgctgat cccgaccagc tgatcgagca gcgcgggcga atactcctgc aacgactcgc
     3481 ggtgattgac cgcatcctcc aggttgacga gctcgtggcg gcgcttgcag aacgcgagga
     3541 agagcgacag gaacaacgtg cagatcagga gccagggcga gattgcgatg gtcgggtcga
     3601 ccccgcgcag cgcttccacg ccggcgatgg cgcgcaggac gaacgagagc gagatgctgg
     3661 cgacgtcgag aaggacgaca cgcttgagcc cgaacgaata caccaagttc aacgcggtga
     3721 atacgcacgc gaccaccagg aaccccggac ccagccggta cgaccacgcg agggcggcaa
     3781 cgatcatgag tgccgcggcg ccgagcgcag tcgagcgctt catcgcgccc gacgcgagcg
     3841 gacgcgcgcg cttgacggga tgcaggcgat ccttgtcgac gtcggcgacg tcgttgacca
     3901 gatagatcgc gcccgagagg atgcagaaca ccacgaaggc ttcgaggctg cgcacgagca
     3961 ggtcgacgtc gcggaagtgg cgcgagaaaa tgagccccgc gaagacgatc aggttcttgg
     4021 tccactgctt ggggcgcatg gacgcgccga tgtagtagac ctgcttcacg tctcgctccg
     4081 cggcgcggct tcgctcgcgc gacccacgtg gtagagctgt gcggtcaccg cgcgcgtcca
     4141 cgcctcgcgc tcggcgcggg tggcgtcggc ggggctgtgc gtcgccagtt tgcgaaacgg
     4201 gtcggcggcc gggaacagag ggctcaagac gcgccgcgcg ccgtcgatgc cgcacaggtc
     4261 gaggtgctcg gagggaaaca ccagtcccgc gcccggcgcg gcgggcgcgg ggtcgcggtc
     4321 gctttcgtcg tcgccgcggg cggccgcgcg ccccgcgtcg tccaagtcga tgggcgcggc
     4381 gggtgccagc gtgtagatac cgcggaacgc gccgcccgcg atttcggcga ggagtgccgc
     4441 cgcctccgat tcgagcgcgt cgaagcgcgt gtgcgacggc ggctccttcg tccacgacgt
     4501 gtcggccagg agcgcggcgg tcaggatgcg cgggaggcgc acgaccgacg ccggttccgc
     4561 acccgcacga ttgagcacgg cggcttccat cgcggcggcc acggccgcgg cgcgatcgcc
     4621 cgggcgcgca ccaccccaaa aggcggccac caccaggcgg cacccgggcc gctgcgtcca
     4681 cagctcccgc gcgagcagat cgctctcccg caccacgtgg tgccacgcgc gctcgtcttc
     4741 gttctccgcg cccgacggct ccacgcgcac cgcgtgcacg accacatcgg gccgcgtggc
     4801 ggcgatcgcg tcgacggccg ccaccggcag cggtcccgcg tagtgcacga cgtcgcgcaa
     4861 ggcgccctcg gacaccgcgc gcggatcgtc gacgtggagt accggcaccg cgcccaccgc
     4921 gcgcaggcgc gcgatcaagg cgccgccgat gcgctcgccg ccgtgcgtca ccagcacgcg
     4981 cgcgccgcgc aaggcagcct gcgaccgcgg cgacgggtcg atcacgccgc ggcgataccg
     5041 ccgcgcgacg cgatcgagcg ccaaggaggc gggttcgcgg gcgccggcgc tgcccggcag
     5101 ccgctcgatg gagagattcg cgcgccgagc gaacgcgagc cagcgctccg cggtgacgtc
     5161 gtcttccccg ggaaggccgt acgcgagcgc gtcgacgcgg tggcgcgcgc acgcatcgcc
     5221 cagtcgctcc gccggtccct cgaccggcac cccgtg
//
LOCUS       DASPGK010000308         8932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_313546, whole genome shotgun sequence.
ACCESSION   DASPGK010000308 DASPGK010000000
VERSION     DASPGK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313546"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VEC56_08655"
     CDS             <1..667
                     /locus_tag="VEC56_08655"
                     /inference="COORDINATES: protein motif:HMM:NF020691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HXV14264.1"
                     /translation="LEESFGRETFDPFLRGYFDAHAFQTMTTERFVAYMKEHLFANDE
                     AKYAELLVDDWIYQPGMPANTPKPKSTRFKQVDAQIAAFGKGTPARFLQTEGWTTNEW
                     QRFLDNLPQPVEHDRLADLESTFHFEKMNAVVQRSWFPNVIASKYEPAYPALENFLLS
                     IGRRYLIRPVYMKLAETPEGLEFARRVYAQARPGYHAITQQGIDPVLKWSEVAMQTPD
                     ERQ"
     gene            664..1137
                     /locus_tag="VEC56_08660"
     CDS             664..1137
                     /locus_tag="VEC56_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXV14265.1"
                     /translation="MKTVVGVISDTHGLLRPEALTALAGSDRVIHAGDVGEEEILEAL
                     RAIAPLTVVRGNVDTQAWCSKLPYGDLLEVDGVRIYVVHDLGTLDIVPASAGVSVVVF
                     GHSHHPDVFTRNGITFFNPGSAGPRRFRLPVTVGRIDVEDGRARAKIVELNLDDD"
     gene            1148..1624
                     /locus_tag="VEC56_08665"
     CDS             1148..1624
                     /locus_tag="VEC56_08665"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV14266.1"
                     /translation="MKETLQRLFAHMRWADDQIYIRCRDAHGEMEVAHLTEALRLYAH
                     IAASERLWFLRLQKQDWTVQKVWPTMSLEACAQLAKENAADFGAYLAGLTEDDFATAI
                     PYTNSKGDSFTTAVGDILIHVATHGVHHRGQIAMTLRRVGIEPPILDYTHWARSVK"
     gene            1683..2261
                     /locus_tag="VEC56_08670"
     CDS             1683..2261
                     /locus_tag="VEC56_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV14267.1"
                     /translation="MKPNSAHDLDDARLVKRYVGSGDEGAFRALYRAHTPAMLAVVWR
                     YLDGDRTDAEDVVQEAWIRASEKLATFRWESALRTWLVGIAINCARNRARTRAANSRE
                     AYLADVVELPAAPPAIDVAAYDVQRAVAHLPEGYREVLLLHDVFGYTHDEIARMLGVE
                     SGTSKSQLSRARSSVRRWLTEKGRIGHERRSE"
     gene            2242..2895
                     /locus_tag="VEC56_08675"
     CDS             2242..2895
                     /locus_tag="VEC56_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXV14268.1"
                     /translation="MSDDPNKNLSDAEQRALRSLGGEIDPSPELEGRVVADLKRRGAI
                     RANGGWIMKISKTAAAAIIVAAAFAGGFAMGSRGETATAPADVATTTPATDANQYMLL
                     MYMRVDPTQAADAPAEEPMNEEAYRAIIDEYRQWALDREKEGRLVSAEKLADDTRVMA
                     GRGDQMTIATSAHSDRALGGYFLITAPSLDDAIALARTHPHLKYGGEVEVRPIETTN"
     gene            2941..4350
                     /locus_tag="VEC56_08680"
     CDS             2941..4350
                     /locus_tag="VEC56_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HXV14269.1"
                     /translation="MRAPSSSWTRRRFLGACGAVTVGFVAYRRAFERSIDALASTATT
                     ARGYGALVADPAAVIDLPQGFHYKVVARTGDEMADGLLVPGLPDAMAAFPGAEGRAIV
                     VCNHECLIDQSAFGSKHERLKMIPAAKLYDNGGGVTPCAGGTTTFVWDTRAQSLVTQH
                     VSLAGTLRNCAGGATPWGSWLSCEETVLKKGLGKEDNIQLTKDHGWVFEVPATAEPSI
                     ADPIPIVAMGRFMHEAVSVDPRTGIVYLTEDRDDGLIYRFLPAQRGDLHAGGRLQALA
                     LASGTKDSRNWPSDKPSLRIGDKHAVKWIDLDDIEAPDDDLRARGYAAGACRFARGEG
                     MWMGEGEVYFVCTDGGRAQIGQVFRYTPSAHEGTAAEAKTPGQLELFIEPNDAERVKA
                     PDNVVVAPWGDVLLCEDGADPNRIIGVTPAGALYTFARSRVPGEFAGATFARDGSTLF
                     VNLQQAGLTLAITGPWRAS"
     gene            4347..5390
                     /locus_tag="VEC56_08685"
     CDS             4347..5390
                     /locus_tag="VEC56_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HXV14270.1"
                     /translation="MRRALVLIAVSALFAAGESRACTTFCFEAGGTRVFGKNYDWNVD
                     DGLVVVNKRGVAKTAFTEDNPAAWTSRFGSVTFNQYGREFPSGGINEKGLVVELMWLD
                     ETMYPEPDRRRALPTLQWIQYQLDNSATVDDVVASDAKVRIADGGSARIHFLVADATG
                     AVATFESLNGRMAVHRGDDLPYPVLTNDMYQVSVTYTQNLDAQEPLTGSSLDRFARAA
                     RGLAPSTKKSNPVDEAFALLDQVAQGEYTQWSIVYDIGERRAHFRTRRQRDVRFVDLD
                     ALDFSCKTPVRVVDVNAAHRGDVSKRLKEYSLDTNRSLVRASFAETEFLRETPPEAVE
                     ALARYPEATTCRP"
     gene            5423..7288
                     /locus_tag="VEC56_08690"
     CDS             5423..7288
                     /locus_tag="VEC56_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXV14271.1"
                     /translation="MHSIDLHTPAMTVAIALAGGMAAQSVAYHLRLPGIVILLMLGVL
                     LGPDVLGVIRPDALGDGLSVIIGFAIAVILFEGGMNLEWRRLRREAAVIRRLVTVGAL
                     VTWGGATCAAFFVMGWDWRLAILFGSLVIVTGPTVVTPLLRRIRVNRNLQTILEAEGV
                     FIDAIGAILAVVAFEFTYGTDEPSFGRAVVHVATRVAIGATVGAIGGGLALVLHRSRR
                     VIPHGMEKVFTLTIAWATFQASNMLAPESGITAAVVAGLVVGNIWTRDQQRLSQLRDF
                     KEQLTVMLIGMLFVLLAASVRLDEITSLGTRGFLVVAALALVVRPLQVWLCTAGSDLT
                     TREKAFISWISPRGIVAASVATLFYERMHATGVAGGGEMRGLVFLVIACTVVVQGITG
                     AAVAEWLGVRRPRGRGFVILGANDLAIALGRELRVAGEEVVLVDANPELSMNAERRGF
                     RVLNGNALDDRILQQSDLDTRKGVVALLPNGGVNLLFARRAIEENRSARVYVAMHRGA
                     ITPAHVKESRARVLCGADLDVDLWASRLRRSGASAEPWRFEGNARDASSSFPVAREAR
                     HVVLPISFARGQVVLPTDDRYEPKKGDVVAWLVFTKRVDEAHEWLTSRGWVRADI"
     gene            complement(7308..7802)
                     /locus_tag="VEC56_08695"
     CDS             complement(7308..7802)
                     /locus_tag="VEC56_08695"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HXV14272.1"
                     /translation="MFPLIVIVAGLVGTALMTGVMWFIHRSGWANADMSRALGSLVTR
                     RYENSLLPGIAIHFAGGCVFAIGYLLVFRSLDLNSIRASVAVGAALGAFHGAAMSFIL
                     MALVAETHPVERFRTAGVEVAAAHVVGHLAYGMGVGLVAGLLGTGKAEATTAFVERAL
                     RALG"
     gene            7929..8513
                     /locus_tag="VEC56_08700"
     CDS             7929..8513
                     /locus_tag="VEC56_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV14273.1"
                     /translation="MAKTRAESGQQNDPARDTALLRRIRGRDEAALSEFYDRYSSFVY
                     SLARSVLRDDGDAEDIVQEVFFRVWERADAFDPARGSPAAWLTTMTRRLAIDRTRSRS
                     FRARGREAPLEAVAGSASGAGGESVTNSAEANEVLAALEQLEPAYREVIRLSYYEGLS
                     HSNIATKLGTPLGTVKSRLREGVTQLRRILAIKA"
     gene            8514..>8932
                     /locus_tag="VEC56_08705"
     CDS             8514..>8932
                     /locus_tag="VEC56_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14274.1"
                     /translation="MDHKIAFELLESYAFGTLDDAERAAVDAHLLTGCTECTARLRDV
                     SELSVRLAQAVPQVEVPARAKERLFERIRTTHHPAKTARASSGRLRLAWSTAGVATAA
                     AGLLLWQTTVLRGELRTTNRLLASTIADTGRLRTELSS"
BASE COUNT         1546 a         2984 c         3006 g         1396 t
ORIGIN      
        1 gctggaggag tcgttcgggc gcgagacgtt cgatccgttc ctgcgcggct acttcgacgc
       61 gcacgcgttc cagaccatga ccacggagcg cttcgtcgcg tatatgaagg agcacctgtt
      121 cgccaacgac gaggccaagt acgcggagct gttggtcgac gactggatct accagcccgg
      181 catgccggcc aacacgccca agccgaaatc gacgcgcttc aagcaagtcg acgcgcagat
      241 cgccgccttc gggaagggaa cgcccgcgcg tttcttgcag accgagggct ggaccaccaa
      301 cgagtggcag cgtttcctcg ataatttgcc gcagccggtc gaacacgatc gcctcgcgga
      361 cctggagagc acgttccact tcgagaagat gaacgcggtc gtgcagcgct cgtggttccc
      421 gaatgtgatc gcgtcgaagt acgaaccggc gtatccggcg ctcgagaact tcttgctatc
      481 gattggacgg cgctacttga ttcgacccgt gtacatgaaa ctcgcggaga cccccgaggg
      541 tctcgagttc gcgcggcgcg tgtatgccca ggcgcggccc ggctaccacg ccatcaccca
      601 gcagggcatc gaccccgtgc tcaagtggag cgaagtcgcg atgcaaactc ccgacgagag
      661 gcaatgaaga ccgtcgtcgg cgtcatctcg gacacgcacg ggctcttgcg gcccgaagcg
      721 ctcaccgcgc tggcgggctc cgaccgcgtg atccacgccg gcgacgtcgg cgaggaagag
      781 atcctggagg cgttgcgtgc gattgcaccg ctcacggtcg tgcgcggcaa cgtcgacacg
      841 caggcgtggt gctcgaagct cccgtacggc gacttgttgg aagtggacgg cgtgcgcatc
      901 tacgtcgtcc acgatttggg cacgctcgac atcgtgcccg cgtccgcggg ggtttcggtg
      961 gtcgtgttcg gccactcgca ccatccggat gtattcacgc gcaacggtat cacgttcttc
     1021 aaccccggga gcgcggggcc gcgccgcttc aggctgcccg tcacggtggg gcggatcgac
     1081 gtcgaagacg gccgcgcgcg cgcgaagatc gtcgaattga atctcgacga cgactaagga
     1141 gagagccatg aaagagacgc tgcaacgact cttcgcccac atgcggtggg ccgacgatca
     1201 aatttacatt cgctgccgtg acgcgcacgg cgaaatggaa gtcgcgcatc tcacggaggc
     1261 gctgcgcctg tatgcccaca tcgccgcgtc cgagcgcctg tggttcctgc gactccagaa
     1321 gcaggattgg accgtccaga aggtgtggcc aaccatgtca ctggaggcgt gcgcacagct
     1381 tgcgaaggag aacgcggccg acttcggcgc gtatctcgcc ggactgacgg aagacgattt
     1441 cgcgaccgca attccctaca cgaactcgaa gggcgattcg ttcacgaccg cggtcggaga
     1501 catcctgatc cacgtcgcga cccacggcgt ccaccaccgc ggacagattg cgatgacgct
     1561 acgccgcgtg ggcatcgagc cgcccatcct cgactacacc cactgggcga gaagcgtcaa
     1621 gtaacgccgc gactcgcggg tcgcgtgcaa cccttcggct ccgaccggac acccacctgt
     1681 tgatgaaacc caacagcgcg cacgatctcg acgacgcgcg cctcgtcaag cgctacgtgg
     1741 gcagcggcga tgagggggcg tttcgggccc tctaccgcgc tcacacgccg gccatgctgg
     1801 cggtggtgtg gcgctacctc gacggcgatc gcacggacgc cgaggatgtg gtgcaggagg
     1861 cgtggattcg ggcctcggaa aagctcgcca cgttccgatg ggagtcggcc ctgcgcacgt
     1921 ggctcgtcgg catcgcgatc aactgtgcca gaaaccgcgc gcgcacgcgc gcggcaaact
     1981 cgcgtgaggc ctatcttgcc gacgtcgtcg agttacccgc ggcgccgcca gcgatcgacg
     2041 tcgctgccta cgacgtccaa cgggcagtgg cgcacctgcc ggaaggctac cgcgaggttc
     2101 tcctgttgca cgacgtcttc ggctacacgc acgacgagat cgcacgcatg ctgggggtcg
     2161 aatccggaac atcgaagagc cagctctcgc gagcacgcag ctcggtgcgg cgctggctca
     2221 ccgagaaagg acgcatcggt catgagcgac gatccgaata agaacttgag cgacgcggaa
     2281 cagagagcgc tgcgctcgct gggcggcgaa atcgatccgt cgcccgaact cgagggtcgc
     2341 gtggtggccg atctgaaacg gcgcggagcg atccgcgcga atggaggctg gatcatgaag
     2401 atttcgaaga cggcggccgc ggccattatc gtagcggcgg cattcgcggg aggtttcgcc
     2461 atgggaagtc gcggcgaaac ggcaaccgct cccgcggacg ttgcgaccac gacgcccgcg
     2521 acggacgcga accaatacat gctgctcatg tacatgcgcg tcgacccgac gcaggccgcg
     2581 gacgcgcccg cggaggagcc gatgaacgaa gaggcctacc gcgcgatcat cgacgagtac
     2641 cgccagtggg cgctcgaccg cgaaaaagag gggcgcttgg tttcggcgga gaaactggcc
     2701 gacgacaccc gcgtgatggc gggccgcggc gaccagatga cgatcgcgac ctcggcgcac
     2761 tccgaccgcg cgctgggcgg ctacttcctg atcaccgccc ccagtctgga cgacgccatc
     2821 gcgctggcca ggacgcaccc gcacctgaag tacggcggcg aagtcgaagt ccggcccatc
     2881 gagactacca actagtccgc ccgttcccgg cgctcgcgtg cgtgtgctag actgccgcgc
     2941 atgcgagcgc cttcttcctc ctggacgcgc cgtcgctttc tcggcgcctg cggtgccgtc
     3001 accgtcggct tcgtcgcgta ccggcgcgcg ttcgaacgat ccatcgacgc cttggcgagc
     3061 accgcgacga ccgcgcgcgg ctacggcgcg ctggtcgcag acccggccgc ggttatcgac
     3121 ctcccgcagg gcttccatta caaggtcgtc gcgcgcaccg gcgacgagat ggccgatgga
     3181 ctcctcgtcc ccggccttcc cgacgcgatg gctgcgtttc ccggcgcaga gggccgcgcc
     3241 atcgtcgtgt gcaaccacga gtgcttgatc gatcaaagtg cgttcggaag caagcatgag
     3301 cgcctcaaga tgatccctgc cgcgaaactc tacgacaatg gcggaggcgt gacaccgtgc
     3361 gcgggcggga cgaccacctt cgtgtgggac acacgcgcgc aatcgttggt gacacagcac
     3421 gtgagcctcg cgggaacgct gcgcaactgc gccggcggtg cgacgccgtg gggctcgtgg
     3481 ttgagctgcg aggagacggt attgaagaag ggcctcggca aggaagacaa catccaactg
     3541 acgaaggacc acggctgggt gttcgaagtc cccgccacgg cggagccgtc gatcgccgac
     3601 ccgattccca tcgtggccat gggccgcttc atgcacgagg ccgtctccgt cgacccgcgc
     3661 accggcatcg tgtatctgac cgaggaccgg gacgacgggt tgatctatcg cttccttccc
     3721 gcacagcgcg gcgacttgca cgcgggcggg cgcttgcagg cgctggccct cgcgagcggc
     3781 accaaggact cacgcaactg gccgagcgac aaaccgagcc tgcgaattgg tgacaagcac
     3841 gcggtcaagt ggatcgactt ggacgacatc gaagctcccg acgacgactt gcgagcgcgc
     3901 gggtacgcgg ccggtgcgtg ccggttcgcg cgcggcgagg ggatgtggat gggggagggc
     3961 gaagtgtact tcgtgtgcac cgacggtggg cgcgcgcaaa tcgggcaagt gttccgctac
     4021 acgcccagcg cccacgaagg caccgcggcg gaagcgaaga cgccggggca gctcgagttg
     4081 ttcatcgagc ccaacgacgc cgagcgcgtc aaggcgcccg acaacgtggt ggtggcgccg
     4141 tggggcgacg tcctcttgtg cgaagacggc gcggatccca accgcatcat cggcgtgacg
     4201 ccggccggcg cgctgtacac cttcgcgcgc tcgcgcgtgc cgggtgagtt cgcgggcgcc
     4261 acgttcgctc gcgacgggtc gacgctcttc gtcaacctcc agcaggcggg cttgaccctc
     4321 gcgatcacgg gcccgtggag ggcaagttga ggcgcgcgct ggttctgatt gcagtaagcg
     4381 cgctctttgc cgcgggcgag tcgcgcgcgt gcacgacctt ctgcttcgag gcgggcggta
     4441 cgcgcgtgtt cggaaagaac tacgactgga acgtggacga cgggctggtg gtcgtcaaca
     4501 agcgcggcgt ggccaagacc gccttcaccg aagacaaccc ggccgcgtgg acctcgcgct
     4561 tcgggagcgt caccttcaat cagtacgggc gcgagttccc cagcggcggt atcaacgaga
     4621 agggactcgt ggtcgagctg atgtggctcg acgagaccat gtatcccgaa cccgaccggc
     4681 gccgcgcgct gcccaccctg caatggatcc agtaccaact cgacaactcc gcgacggtcg
     4741 acgacgtggt ggcgtcggac gccaaggtac gcatcgcgga cgggggatcg gcccgcatcc
     4801 actttctcgt cgccgacgcg accggagcgg tcgcgacctt cgagagtttg aacggccgga
     4861 tggcggtcca tcgcggcgac gacctgccat accccgtgct gaccaacgac atgtaccagg
     4921 tctcggtgac ctacacccag aacctcgacg cgcaagagcc cctcacgggc tcgtcactcg
     4981 atcgcttcgc gcgcgcggcg cgcgggctcg cgccgtcgac gaaaaagagc aaccccgtcg
     5041 acgaggcctt cgcgctcctc gaccaagtcg cgcagggcga gtacacgcag tggagcatcg
     5101 tgtacgatat cggcgagagg cgcgcgcact ttcgcacgcg ccgccagcgc gacgttcgct
     5161 tcgtcgacct tgacgcgctc gatttctcgt gcaagacacc ggtacgggtc gtcgacgtga
     5221 acgcggcaca tcgaggcgac gtgagcaagc gcctgaagga gtactcgttg gacacgaacc
     5281 gaagcctcgt gcgcgcgtcg ttcgcagaga ccgaattcct gcgcgagacg ccgcccgagg
     5341 ccgtcgaagc gctcgcacgc tacccggaag cgacgacgtg cagaccgtag ccgaaaccac
     5401 gccccgttag tcgaagtcac cgttgcactc gatcgatctc catactcccg cgatgacggt
     5461 cgcgatcgcc ctggcgggcg gaatggccgc gcagtcggtg gcgtaccacc tgcgcctgcc
     5521 cggcatcgtc atcctgctca tgctcggtgt gctgctcggc cccgacgtcc tgggcgtcat
     5581 tcgccccgac gcgctgggcg acggactctc ggtcatcatt ggcttcgcca tcgccgtcat
     5641 tctgttcgaa ggcggcatga atctcgagtg gcggcggctg cgccgcgagg ccgcggtcat
     5701 tcgccggctc gtcaccgtcg gcgccctcgt cacctggggc ggggccacgt gcgccgcgtt
     5761 cttcgtcatg gggtgggact ggcgcctcgc gattctcttc ggatctctgg tgatcgtcac
     5821 cggtcccacc gtggtaacgc cgctcctgcg acgcatccgc gtcaatcgca acctgcaaac
     5881 cattctcgag gccgagggcg tgttcatcga tgccatcggc gccattttgg ccgtggtcgc
     5941 cttcgaattc acctacggca ccgacgagcc gtcgttcggc cgagccgtgg tgcacgtggc
     6001 gacacgcgtc gcgatcggcg cgaccgtggg cgccatcggc ggcgggctcg ccctggtcct
     6061 gcaccgttcg cgccgcgtga tcccgcacgg catggagaag gtgttcacgc tcaccatcgc
     6121 gtgggcgacc ttccaggcca gtaacatgct cgcgcccgaa tcgggtatta ccgcggcggt
     6181 cgtcgcggga ctggtcgtgg gcaatatttg gacacgcgac caacagcgct tgagccagct
     6241 ccgcgatttc aaagagcagc tcaccgtgat gctgatcggg atgctgttcg tgctgttggc
     6301 ggcgagtgtt cggctcgacg agatcacgtc gctaggcaca cgcggcttcc tggtcgtcgc
     6361 ggcgctcgcg ctggtcgtgc gccctttgca ggtgtggctg tgcacggcgg ggtcggatct
     6421 caccacgcgc gaaaaggcgt tcatctcctg gatctcgccg cgcgggatcg tggccgcgtc
     6481 ggtggcgacg ctgttctacg agcgcatgca cgcgacgggt gtcgcgggcg gcggggaaat
     6541 gcgcgggctc gtgttcctcg tgatcgcgtg caccgtggtg gtgcagggga tcacgggcgc
     6601 ggcggtcgcg gagtggctgg gagtacgcag gccgcgcggt cgcggcttcg tcatcctggg
     6661 cgcgaacgac ctcgcgatcg cgctcggacg cgagctgcgc gtggcgggcg aagaagtggt
     6721 gctggtcgac gccaaccccg agttgagcat gaatgcggaa cgcaggggct tccgcgtgct
     6781 gaacggaaac gcgctggacg accgcatcct gcagcagagt gatctcgaca cccgcaaggg
     6841 cgtggtcgcg ctcctgccca acgggggcgt caacctgctg ttcgcgcggc gcgctatcga
     6901 agagaatcga agtgcgcggg tctacgtcgc catgcaccgc ggagcgatca cgccggcgca
     6961 cgtcaaggaa tcgcgcgcgc gtgtgctgtg cggcgccgac ctcgacgtcg atctgtgggc
     7021 cagccggctg cgacgaagcg gagcgtccgc ggagccgtgg cgcttcgagg gcaacgcgcg
     7081 cgacgcgtcg tcgtcgttcc cggtcgcgcg cgaggcgcgc cacgtcgtcc tgccgatttc
     7141 attcgcgcgc ggccaggtcg tcctgccgac cgacgaccgc tacgagccca agaagggcga
     7201 cgtggtggcc tggctggtat tcaccaaacg cgtcgacgaa gcccacgagt ggctgacgtc
     7261 gcgcggctgg gtgcgcgcgg acatctgacg cggaaaaaac ccctcggcta ccccagcgcg
     7321 cgaagcgcgc gctcgacgaa ggccgtcgtc gcttccgcct ttcccgttcc cagcaacccc
     7381 gcaaccagtc cgacccccat cccgtaggcg aggtgtccga ccacgtgggc ggcggccacc
     7441 tcgacgcccg ccgtgcgaaa acgctcgacc gggtgagtct cggccaccag tgccatcaag
     7501 atgaagctca tggccgcgcc gtggaatgcg cccagggcgg ccccgaccgc gaccgacgcg
     7561 cggatcgagt tgagatccag gctgcgaaag acgagtaagt agccgatggc gaagacgcac
     7621 ccccccgcga agtggatggc gatgcccggg agcaaggagt tctcgtaacg acgggtgacg
     7681 aggctgccga gcgcgcgcga catgtccgcg ttcgcccagc cggagcggtg gataaaccac
     7741 atgacgccgg tcatcagcgc ggtgccgacg agcccggcga cgatcacgat caagggaaac
     7801 atgaacggcc tccctctatg ttggcccgag cataacccac ggtgccatcg ggcgtaaaga
     7861 cgcgccgcgc ccgccgtgct acaatgcccc caaccgctcc aaaacaccga ttggcttcgt
     7921 attagagtat ggcgaaaacg cgggcagaat ccgggcagca aaacgacccg gcgcgcgaca
     7981 cggccttgtt acgccgcatc cgcgggcgcg acgaagccgc cctgtccgag ttctatgacc
     8041 gatactcgag cttcgtatac agcttggccc gcagcgtcct gcgcgatgac ggcgacgccg
     8101 aggacatcgt gcaggaggtg ttcttccgcg tgtgggaacg cgcggatgcc ttcgaccccg
     8161 cgcgcggcag cccggccgcg tggctgacga ccatgacgcg ccgcctcgcc atcgaccgta
     8221 cccgctcgcg ttccttccgc gcccgcgggc gcgaagcccc gctggaagcg gtggccggct
     8281 cggcgtcggg cgcaggcggc gaatcggtga cgaactcggc cgaggccaac gaagtgctgg
     8341 ccgcgctgga acagctcgag cccgcgtacc gcgaggtgat tcggttgtcg tattacgaag
     8401 gcttgtcgca ctcgaacatc gcgaccaagc tgggaacccc gttggggacg gtgaagagcc
     8461 ggttgcgcga aggagtgacg cagctgcgtc gcatcctcgc gatcaaagca taaatggacc
     8521 acaagatcgc attcgagctg ttggagtcgt acgcgttcgg caccttggac gatgccgagc
     8581 gcgcggcggt cgacgcgcac ctgctcaccg gttgcacgga atgcaccgcg cgcctgcgcg
     8641 acgtgtccga gttgtcggtg cgcctggcgc aagccgtgcc ccaggtcgaa gtccccgcgc
     8701 gcgccaagga gcgcctgttc gagcgcattc gcacgaccca ccacccggcc aagacggcgc
     8761 gcgcgtcgtc gggccgattg cgcctcgcgt ggagcacggc cggagtggcg accgcggcgg
     8821 ccggccttct cctgtggcaa acgaccgtgt tgcgcggcga gctgcgcacc accaaccgcc
     8881 tgctcgcctc gaccatcgcc gacaccggac gcttgcgcac cgagctctcg tc
//
LOCUS       DASPGK010000309         6350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1482478, whole genome shotgun sequence.
ACCESSION   DASPGK010000309 DASPGK010000000
VERSION     DASPGK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482478"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VEC56_08710"
     CDS             <1..847
                     /locus_tag="VEC56_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXV14275.1"
                     /translation="NVTGYNLTQLIVGSEGTLAIVTKIYLRLLPLPKYRTMLLAPFDS
                     LEEPARAVPAIMHAGIVPSVLEFMERDAVEITERHLGKSFPHAEAAAHLMIELDGNDE
                     GVMAREAERVAEIVLAHGATDVLLADTDAKMGELWAMRRAFGAAVKSESIYKEEDTVV
                     PRNQLVALVKGVKEIAARHGLRTVCYGHAGDGNLHVNILKMDMSEERWNTALDPAIRE
                     IFALTVKLGGLISGEHGIGYVQKDYMPIAYTAAEIALMRAVKHAFDPMGILNPGKLLP
                     SAGAD"
     gene            complement(851..1384)
                     /locus_tag="VEC56_08715"
     CDS             complement(851..1384)
                     /locus_tag="VEC56_08715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HXV14276.1"
                     /translation="MATRVVTTALDQPRSWWERTYLGEVFRGLGITMAHMIKNLADLR
                     HDRVGMTYQWPEEPKPLPDTFRGEHRLMHRPDGTPRCVACNCCATACPANCIEITGAD
                     TGHDGVEKYPIKFDIDILQCVYCGMCVEACPCDAIRMDTGKITDAYFEGPVKILDIDY
                     LMKNHPDDKSPISEAIY"
     gene            1504..3192
                     /locus_tag="VEC56_08720"
     CDS             1504..3192
                     /locus_tag="VEC56_08720"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HXV14277.1"
                     /translation="MSENREVLEVDILFVGAGAASLSGALRLHQLIEAHNARVAQTGQ
                     GSALSPMIAVVEKGSEVGAHSLSGAVLDPIALRELIPDYESKGCPIEKKVEAEDVYYL
                     TPKRALRFPVTPPPLRNHGLPIVSLARFNRWLGGLVEGAGVNVFAGFAGVEILEENGA
                     VVGIRTGDKGIDRHGQRKPNFEPGIDLRAKLTIFGDGPRGYLSKQLIARHGLLEGKSI
                     QTFETGVKEVFELPPGRTDVGRVIHTMGHPFRADSVGGTFIYTMADNLVAVGLVTPLD
                     YSDPFINPHEQLQQFKEHPTIRKLLAGGKSAYYGAKVISAAGYHSIPKLYTNGALLIG
                     EAASLVDMARLKGIHLAMKSGMLAAETAFHALIADDFSSKALAPYEHAIYDSYVGKQL
                     YKVRNFHRAMSLGLPRALMHVGLQQMTGGADILGKGHGKEDRYTFKSVAEYYGVDIDL
                     PPPRKYDGREFLDKLQDVYLSGTMHGEDQPSHLLVLDTKKCYDTCVATYRYPCNRFCP
                     ANVYEMVASDGGALRLQVNFANCVHCQTCDIKCPLDNIRWTPPEGGQGPNYTVL"
     gene            complement(3266..3691)
                     /locus_tag="VEC56_08725"
     CDS             complement(3266..3691)
                     /locus_tag="VEC56_08725"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HXV14278.1"
                     /translation="MNPKFFTVEEANALIGFLENSLERIRRNRQRYLWLLEEIAILKL
                     IVECGADESNSDSVDLEEKARELKAVEAEIEKARGSVRDAGCILKDEEKGLVDFFSIQ
                     SNTVVYLSWKRGEDTVKFWRSIRDADSNVRRPLEGSPST"
     gene            3965..5086
                     /locus_tag="VEC56_08730"
     CDS             3965..5086
                     /locus_tag="VEC56_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase domain-containing protein"
                     /protein_id="HXV14279.1"
                     /translation="MPHPSRTSPTSLVVNRPIDLGEIEAVGFDLDHTLALYDDAAVNA
                     LAMAEALHLLVEHRGYRARDIALAAPVGDAGTARALAIDLANAHVVKLDASRRVRVAR
                     RAGEWLQDDEIGRAHPERVPERDDVVHPLSSAFDVPTLWLFEAVTRGHAAAEHVAPAL
                     DAVRVCRDVRQMLDLSHTRGELKSHLARDLERFVTPVAGATERLLEWRRAGKRLFVVT
                     NSDLAFATEVLDLVIGPAWRDIFSVVSTSSGKPSFFAAADARAAAPRGDVVREGATAR
                     ELEAVLGARGERVLYVGDNARADIAPARSFGWKTVHVVAELSAGDQPAEGWGSPFLAG
                     GEPSWFARVVGEYADAVCDRVDRLLACEPDRLLDAGESA"
     gene            5151..5744
                     /locus_tag="VEC56_08735"
     CDS             5151..5744
                     /locus_tag="VEC56_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXV14280.1"
                     /translation="MTLKHDITMPALPATASRSNRIALGATLAIAFAGLTTLGAYIVI
                     PIQPVPITMQTLFVLLAGASIGRGWGSTSQWLYVGLGAAGVPLFAGGASGLGILGGPT
                     GGYLLAFLVTPWVVGTLLRRSERLWWQALSFLVGKVVILALGVTHLTLFYTHDLAQAI
                     TVGVLPFLPGAAFKIVAALSIYRSSTALVRHYRARQS"
     gene            5875..>6350
                     /locus_tag="VEC56_08740"
     CDS             5875..>6350
                     /locus_tag="VEC56_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14281.1"
                     /translation="MRKFLTVMIAVVCAGSASADHIGLYRDPAGQYCALQSPPVGPPP
                     FALYVIHRFNAGASGSQFRVLDTTGFFATTQSTIGPTLGTWNTDWSISYGGCLIGDLQ
                     IATLNFLYFGTPLLCTNWMGIHPAPTSAIVGEIIYANCAQEFETATGGRIWFGPAS"
BASE COUNT         1071 a         2096 c         2014 g         1169 t
ORIGIN      
        1 gaacgtcacc ggctacaacc tcacgcagtt gatcgtgggg tccgagggca cgctcgcgat
       61 cgtcaccaag atctacctgc gcctcttgcc gcttcccaag taccgcacga tgttgctcgc
      121 tcctttcgac tcgctcgaag agcccgcgcg cgcggtgccc gccatcatgc acgcgggtat
      181 cgtcccatcg gtgctggagt tcatggagcg tgacgcggtg gagatcaccg agcgccactt
      241 gggcaagtcg tttccccacg cggaggcggc ggcccacctc atgatcgaat tggacggcaa
      301 cgacgagggc gtgatggcgc gcgaagccga gcgggtcgcg gagatcgtgc tcgcgcacgg
      361 cgccaccgac gtcctcttgg ccgacaccga cgccaagatg ggcgagctat gggcgatgcg
      421 gcgcgcgttc ggcgccgccg tcaagtcgga gtcgatctat aaagaagagg acacggtggt
      481 gccccgcaac cagctggtgg cactggtcaa gggtgtgaag gaaatcgcgg cgcgccacgg
      541 cctgcgcacc gtgtgctacg ggcacgccgg cgacggcaac ctgcacgtca acatcctcaa
      601 gatggacatg tccgaggagc gctggaacac cgccctcgac cccgccattc gcgagatctt
      661 cgcgctcacg gtgaagctgg gcgggctcat ctcgggcgag cacgggatcg gctacgtcca
      721 gaaggactac atgccgattg cctacacggc cgcggagatc gcgctcatgc gcgcggtcaa
      781 gcacgcgttc gatccgatgg gaatcttgaa tccggggaag ctcttgccgt cggcgggcgc
      841 cgactagggg ctagtagatg gcttcgctga tggggctctt gtcgtcgggg tggttcttca
      901 tcaagtaatc gatgtcgagg atcttgaccg gtccctcgaa gtacgcatcc gtgatcttgc
      961 cggtgtccat gcgaatcgcg tcgcacgggc acgcctcgac gcacattccg cagtagacgc
     1021 actgcaggat atcgatgtcg aacttgatcg ggtacttctc gacgccgtcg tgaccggtgt
     1081 cggcgccggt gatctcgatg cagttggccg ggcacgcggt cgcgcagcaa ttgcacgcga
     1141 cacagcgcgg ggtcccgtcg ggacggtgca tcaagcggtg ttcgccgcgg aacgtgtccg
     1201 ggagcggctt gggttcttcg ggccactggt aggtcatgcc cacccggtca tggcgcaggt
     1261 ccgccaggtt cttgatcatg tgcgccatgg tgatgcccaa gccccgaaag acctcgccca
     1321 ggtaggttcg ctcccaccag gaacggggtt gatcgagggc cgtggttacg acgcgtgtgg
     1381 ccataggacg gtctaatgta gccgaaccgc gccgattgtc aagggctacc gttgcccttg
     1441 tattttgacg aaccgcttac cgagtcgtta tgatgcccgg gtgtggccaa cgacaggagt
     1501 ctcatgagcg agaatcgtga agtcttggaa gtcgacattc tgttcgtcgg agcgggtgcc
     1561 gcctcgttgt ccggcgcgct tcgcctccat caattgatcg aggcgcacaa cgcgcgcgtc
     1621 gcgcagaccg gacagggctc ggcgctctcc cccatgatcg ccgtggtcga aaaaggcagc
     1681 gaggtgggcg cgcattcgct ctcgggcgcg gtgctcgacc cgatcgccct gcgcgagctc
     1741 atccccgact acgagagcaa aggctgcccg atcgagaaga aggtcgaagc cgaagacgtc
     1801 tactacctga cgccgaagcg cgcgctgcgc ttccccgtca caccgccgcc gcttcgcaac
     1861 cacggactcc ccatcgtctc gctcgcgcgg ttcaatcgct ggctgggcgg gctggtcgag
     1921 ggcgcggggg taaacgtgtt cgcgggcttc gcgggcgtcg agatcctgga agaaaacggc
     1981 gccgtggtcg ggattcgcac cggcgacaag ggcatcgacc ggcacggcca gcgaaaaccc
     2041 aacttcgagc ccgggatcga cctgcgcgcc aagctcacca tttttggcga cggcccgcgc
     2101 gggtatctct cgaagcagct catcgcgcgc cacggcttgc tcgaggggaa atcgatacag
     2161 actttcgaga ccggtgtgaa ggaagtgttc gagctcccgc cgggacggac cgacgtgggc
     2221 cgcgtgatcc acaccatggg ccatccgttt cgcgccgact cggtgggcgg cacgttcatc
     2281 tacaccatgg ccgacaacct ggtcgcggtc gggctcgtca ccccactcga ctacagcgat
     2341 ccgttcatca atccgcacga acagctgcag cagttcaagg agcaccccac gatccgcaag
     2401 ctcttggcgg gcggaaagag cgcgtactac ggcgccaagg tgatcagcgc cgcgggatac
     2461 cactccatcc cgaagctgta cacgaacggc gcgctcctga tcggcgaggc ggcttcactg
     2521 gtcgacatgg cgcgcctgaa gggcatccac ctcgcgatga agtccggcat gctcgcggcg
     2581 gagaccgcgt tccacgcctt gatcgccgac gatttttcct cgaaggcact cgcgccgtac
     2641 gagcacgcga tctacgacag ctacgtcggc aaacagctct acaaggtgcg caacttccac
     2701 cgcgccatgt cgctgggact gccgcgcgcg ctcatgcacg tcggcctgca gcaaatgacc
     2761 ggcggcgccg acattcttgg caaggggcac ggcaaggaag accgctacac cttcaagagt
     2821 gtcgccgagt actacggcgt cgacatcgat ctgccgccac cgcgcaagta cgacggccgc
     2881 gagttcctgg acaagctcca ggacgtttac ctatcgggga ccatgcacgg cgaagaccag
     2941 ccgtcgcacc tgctcgtgct cgacaccaag aagtgctacg acacgtgcgt cgccacgtat
     3001 cggtatccgt gcaatcgatt ctgcccggcc aacgtgtacg agatggtcgc cagcgacggg
     3061 ggtgcactgc ggctgcaagt gaacttcgcc aactgcgtgc actgtcagac gtgcgacatc
     3121 aagtgcccgc tcgacaacat tcgctggacg ccgccggagg gcgggcaggg accgaactac
     3181 accgtcctgt agccgacaaa aagacgccgc cggaatcgcg ttccggcggc gttgaaattc
     3241 aagtcgtgga tgcccgcgtc tacctctacg tggacggaga accctccaac gggcggcgca
     3301 cgttgctatc cgcgtcgcgg atcgatcgcc agaatttcac cgtgtcttcg ccgcgcttcc
     3361 aggacaggta cacgaccgtg ttgctctgga tcgagaagaa gtcgaccagg cccttctcct
     3421 cgtccttgag gatgcagccc gcgtcgcgca ccgacccgcg cgccttctcg atctccgctt
     3481 ccacggcctt gagctcacgg gccttctctt cgaggtcgac cgagtcgctg ttcgattcgt
     3541 cggcgccgca ctcgacgatc aacttcagaa tggcgatttc ctccagcagc cacaggtagc
     3601 gctggcggtt gcgccggatt cgctccagcg aattctccag gaagccaatg agggcgttcg
     3661 cttcctcgac ggtgaaaaac ttggggttca tgcgaactcc ttcaactaca ggacacaccg
     3721 ctcccggcgg cgcctaacca gtgccgattt agcccttagc gtttccccac acgtccctct
     3781 attccttgct atttccatac gtatcccgat cacttcggaa aagcaaggcc gatttcccaa
     3841 gttgtcatgt gcattgtttc acacgagttt ttggcgtcca aatcggggtt ttcgcgcggt
     3901 cgcacggtgg aaaaacggcg cgttgaccgg cccgcggcgg gctggtagcg ttaccggttc
     3961 caccatgccg cacccatcga ggacctcccc cacgtccctg gtcgtcaacc gccccatcga
     4021 cctcggcgag atcgaagccg tcggcttcga cctggaccac acgctcgccc tatacgacga
     4081 cgcggccgtc aacgcgctgg ccatggcgga ggcactgcac ttgctggtcg agcatcgcgg
     4141 ttaccgcgcg cgcgatatcg cgctggctgc gcccgtgggg gacgctggga ccgcgcgggc
     4201 gctggccatc gacctcgcca atgcgcacgt cgtcaagctg gacgcgagcc ggcgggttcg
     4261 ggtcgcgcga cgcgcggggg agtggctcca agacgacgag atcggtcgcg cgcacccgga
     4321 gcgcgtcccc gagcgcgacg acgtcgtgca cccgctttcg tccgcgttcg acgtgcccac
     4381 gttgtggctc ttcgaagcgg ttacgcgcgg ccatgcagcg gccgagcacg tcgcaccggc
     4441 actcgatgcc gtgcgcgtct gccgcgacgt tcgccagatg ctcgatctct cgcacacgcg
     4501 cggcgagttg aagtcccatt tggcccgcga cctcgagcgt ttcgtaacac ccgtcgcggg
     4561 tgccaccgaa cggctgctcg aatggcgacg cgcgggcaaa cgactcttcg tcgtgacgaa
     4621 ttcggacctc gccttcgcga ccgaggtgct cgacctcgtc atcggcccgg cgtggcgcga
     4681 catcttctcg gtcgtgtcga cctcatcggg aaagccatct ttcttcgcag ccgcggatgc
     4741 gcgcgcggcg gcgccgcgcg gcgacgtcgt ccgcgagggt gcgaccgcgc gcgagttgga
     4801 ggcagtgctc ggtgcgcgcg gcgagcgcgt gctctacgtc ggggacaacg cgcgggccga
     4861 catcgctccg gcgcgatcgt tcggatggaa gacggtccac gtcgtcgctg aattgtcggc
     4921 gggagatcaa ccggccgagg gctggggctc gccgtttctg gccggcggag agccgagctg
     4981 gttcgcgcgc gtggtcggcg aatacgccga cgccgtgtgc gatcgcgtcg atcgcttgct
     5041 cgcctgcgag ccggaccggc tcctggatgc gggtgaaagc gcttgacacg aaacgccgcg
     5101 acgtcgaaca tgggtgtcct tgggcgcctc cgagacgtcc ttggtcacgt atgacgctga
     5161 aacacgacat caccatgccc gcgcttcccg cgaccgcgtc gcggagcaac cgcatcgcct
     5221 tgggtgccac actcgcgatc gcgttcgccg ggctcaccac cctgggcgcg tacatcgtga
     5281 ttcccatcca accggttccc atcacgatgc agaccctgtt cgtccttttg gcgggtgcgt
     5341 cgatcgggcg cgggtggggt tcgacgagcc agtggctcta tgtcgggctg ggtgccgcgg
     5401 gcgtgccgct cttcgccggc ggggcgagcg ggctcgggat cctcggcgga ccgacggggg
     5461 gatacctcct cgcgttcctc gtcaccccgt gggtggtcgg aacactgctt cgacgctcgg
     5521 agcggctgtg gtggcaggcg ctctcgttcc tcgtcggcaa ggttgtcatc cttgccctcg
     5581 gcgtgactca tctgacgctc ttctacaccc acgatctggc acaggcgatc accgtcggcg
     5641 tgcttccctt cctccccggc gccgcgttca agatcgtcgc ggcactctcg atctaccgct
     5701 cctcgaccgc actcgtgcgg cactaccgag cccgccagag ctagtccagc tttcttccgc
     5761 tcccacctac caatcccgtc tcccgcgggt ggacgttcgt tgctcaggcg atatgatata
     5821 atcgtcatgg aagcgctgct ccacgacgtc ttggcttcag ccgggaggga caccatgcgc
     5881 aagtttctaa ccgtgatgat cgcagtggtg tgtgcgggtt ccgcgtcggc ggaccacatt
     5941 ggtttgtacc gcgatcccgc cggccagtac tgcgcgttgc agagtcctcc ggtcggtcct
     6001 ccccctttcg cactgtacgt gattcacagg ttcaacgccg gtgcctcggg gtcgcagttc
     6061 cgcgtgttgg atacgaccgg attctttgcc accacgcagt cgaccattgg tccgacgctc
     6121 ggtacatgga acaccgactg gtcgatctcg tatggcggtt gcttgatcgg ggacctgcag
     6181 atcgcgaccc tgaacttcct ctatttcggc acgccgctgc tgtgcaccaa ctggatgggg
     6241 atccatccgg ctccgacctc ggcaatcgtg ggcgagatca tatacgcgaa ttgcgcccag
     6301 gagttcgaga cggctaccgg cggacggatc tggttcggtc ctgcttcgca
//
LOCUS       DASPGK010000310        11669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1717986, whole genome shotgun sequence.
ACCESSION   DASPGK010000310 DASPGK010000000
VERSION     DASPGK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717986"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VEC56_08745"
     CDS             <1..556
                     /locus_tag="VEC56_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14282.1"
                     /translation="TVGFALWSFGVWSMVAHHEVSAHVGSRSFRKTLPSVVVIGVVFV
                     GVTVALGRGLATLTDTLPPGMITRGATLAVIAVTAGVQALLVFAPVALRLRGGGPFAA
                     IRSSARYALRRFGATAMLVATVLLVHLPLDGLIANTDVIAARFHPEAVFQLMLASVVL
                     EVITAFVLFAGTVGLALPEEGGMR"
     gene            553..2100
                     /locus_tag="VEC56_08750"
     CDS             553..2100
                     /locus_tag="VEC56_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14283.1"
                     /translation="MTRATLTLLAFVTLLSSCASHDDRVARYRLEQKLWRAQFYQRRI
                     NISVVRASQNDTRMAIAAFNAVLADDPLAQPAAATWRPDVVADIAQIHLASRVALANL
                     YFLSERYADAGKMYERTLEAAGQGLGGSLEARLGAARSMYLAGETTEVMRQCAEMFRQ
                     ISTSEEFWSGRFVPDGVFVDIPVVLARMYRETGDGAAYAEYRALAGEFYGRVAATWPG
                     TPIAWQATLGEIQLRLVDHDWRGAAGQIEAILAAPNPQRDLEGLRLLLGEIYAFALGE
                     PARADAILADVESRAAGTAAAYAARYDRGALMLETGDEDGAMAVFRALEHDKATPGDV
                     AARAMFGRATILERRGAWDEALQLLRRMQQLYPHAPASIEAPILVTRHYVATGEHALA
                     ERTLERAREYYLSLIDRNSPYPGDRLGVQDALAASYATAGRAAEGAALLGSGPSAWDD
                     ASTAAGLLKSADVYATVLGDSARASEMLKKCIERFPETRYSRVAARRLDEIEHGRGAS
                     EERPLEE"
     gene            2419..3708
                     /locus_tag="VEC56_08755"
     CDS             2419..3708
                     /locus_tag="VEC56_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV14284.1"
                     /translation="MRRNRPNWRSATILGLALIGLSGPFADVRAQQGAGTHAAPFLKI
                     PVGARLMSSPDAVAGLSPDATLMYSNPAFLSGLPRAEAFITTSEWLDNLTFTSAGVAM
                     PIGAQGTVLGIGATFLYSGGVQGYDAALNPVSEESFYDVGFDVTVSHAFRGTGLALAA
                     GTTIIREHVLPTDGSGYAFHLGASYWMGPNLFHAAARDLGGTVSFDSESWNVAPEYSF
                     GGGRIFASSLGQFFAGAQFTDSDAYGTRVQLGVDYQFNPMLTLRSGFTDNMDSSQRGA
                     PFQAGFGLHYGVMTLDYAYTPQEYFSSVHSFSLSYAFGGPSSQGGRLTVPTGDFSPPI
                     ADSEPVPPPSRLAVPKETSPSRSGGSYLLLAGSHAWLESARAEVRALQLLQIPAKIES
                     NGSRYRVVVGRFKSADDAEQARREYASSGHAFTVLAE"
     gene            4148..4897
                     /locus_tag="VEC56_08760"
     CDS             4148..4897
                     /locus_tag="VEC56_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXV14285.1"
                     /translation="MIPWNRSSAVVRVRPADRVRHPYDVIGRLGWKRALDIVLSFVGL
                     VVASPVMVAAAVLIKTTSPGPVFYRQERIGINRRGRDRRGSSGRADMDRRRRDRRVLV
                     NFGRPFTIYKFRTMVVDAERGTPPMWAKERDPRITLVGRVLRKTRIDELPQFYNVLRG
                     EMSIVGPRPERAYFIGRIEKDLPEFQLRLRTKPGITGLAQVELGYTNTDDGLRDKLHL
                     DLEYIRRLSFWTDLKILFRTVFVVFTGKGAC"
     gene            5034..5990
                     /locus_tag="VEC56_08765"
     CDS             5034..5990
                     /locus_tag="VEC56_08765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV14286.1"
                     /translation="MLLRVVANTKIPMELTMPVEASKPRVSVGMPVYNGKAFLAQSIE
                     SVLGQTYANLELIVSDNGSTDGTPEIAQDYARRDRRVRFVRTEENRGAAWNYRRTFDL
                     ARGEFFRWAPADDLFAPDSIAVCVAALDADPDAVLCYPKTELIDPEGRVIRGYDDNLD
                     LRASDPAERLARAMAQIGLVNVIYGLMRTAALGKTRLMGTFVGADEVLVFELALQGKF
                     LELPTSRFYRRIHENAFSQMATTEQKQAFFDPRKTGRFSPRLWEHYRQCLHGVVHAPI
                     SAAAKWRAIGVLVRSAIAVRRDLMREVAAGARRAARPKLFGL"
     gene            6016..7152
                     /locus_tag="VEC56_08770"
     CDS             6016..7152
                     /locus_tag="VEC56_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV14287.1"
                     /translation="MTRIDGARVEPLSDLLNRDLDYVTTGLRDELAAMAGSRVLITGG
                     AGFLGYYMVQGILHWNDRANEKDRIRVSVFDNYVRGVPAWLEALRGRADLTLTRFDVR
                     EPLPDPMPEFEYIIHAAGIASPIYYRLHPLETMDANIDGLRNLLEYARARVESKRDFR
                     GFIFYSSSEIYGDPAPDQIPTREDYRGNVSCTGPRACYDESKRYGETLCVVFARHYGV
                     PVKMVRPFNNYGPGLKISDRRVIPDFARDVMNGRDIVMLSDGKPKRTFCYVADAVVGY
                     YKALVRGGIGEAYNVGVDRPEISMRELAEKVVATARDLFGYAGRVVMQAPTESDYLVD
                     NPNRRCPDLTKSRAQLGYDPTILVDEGLRRSLVWYHYHREAEDA"
     gene            7152..8486
                     /locus_tag="VEC56_08775"
     CDS             7152..8486
                     /locus_tag="VEC56_08775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXV14288.1"
                     /translation="MRVSIIGAGYVGIVTGACLAEKGHSVVCVEVDAAKVERIRRGEP
                     PIHEEGLAELLRRHSGTAFRATTSLAEAVAETDITLIAVGTPFDGRAIDLTYVTRAAR
                     EIGELLARKRERHTVVVKSTVVPGTTDGPVREALESASGKKAGEDFGLGANPEFLTEG
                     TAVADFMSPDRIVIGGIDARTLDALSQLYAGFEGVPLIRTNNATAEMIKYASNSLLAA
                     MISFSNEIADLCSAIGGIDAKDVMAGVHESAYLNTVSGHARARAPIASFLEAGCGFGG
                     SCLPKDVSALVAHGGSKGVDMRVLRSVLEVNRGRPDRVIEGLKRRVPSLQGAAVAVLG
                     LAFKPDTDDVRESPAFPIIDRLLAEGAAVTAYDPVATEPARRALAGKPVRYAQTLEGA
                     LREADVVVLVTRWREFVAVPELLRKLGRTPLVFDGRRMLDKNAISRYEGIGL"
     gene            8523..9305
                     /locus_tag="VEC56_08780"
     CDS             8523..9305
                     /locus_tag="VEC56_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HXV14289.1"
                     /translation="MKVVLFCGGQGMRIREYSDEIPKPMVPIGYRPILWHVMKYYAHF
                     GHKEFILCLGYKADVIKNYFLNYDECLSNDFTLSGAGKVELANSDIHDWKITFVDTGV
                     NTNIGGRLQAVRKYVENDDVFLANYSDNLTDLPLPEMIRDVGSSPRVATFLAVRPSQS
                     FHMVSFDDKRSVDAITHVSASEVFINGGYFVLRKEIFDYMKNGEELVEKPFERLIAKK
                     LLSAYRYEGFFVSMDTFKERQTLESMYTRGEAPWELWAGKRR"
     gene            9312..9953
                     /locus_tag="VEC56_08785"
     CDS             9312..9953
                     /locus_tag="VEC56_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HXV14290.1"
                     /translation="MKTLAVGSIGSILCLGAHSDDIEIGCGGTVLKLVESNPGLSVHW
                     VVFSATGERGGEARASADRFLARAGRKTVELHQFRDGFFPSEHSKIKTYFEELKSRVQ
                     PDAVFTHARADLHQDHRAICELTWNTFRDHLVLEYEVPKWDGDLGAPNVYVPLDERHA
                     NEKIAILLECFASQRGKHWFDQETFAGLMRLRAMECNAPSRRAEAFYGRKVVL"
     gene            9950..10756
                     /locus_tag="VEC56_08790"
     CDS             9950..10756
                     /locus_tag="VEC56_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14291.1"
                     /translation="MSAVARPVAVEWLYEFVRYRELFFFLVWRDVKIRYKQTILGAAW
                     AIIQPFALMVVATFAFGRAAGIDSEGAPYPVFAYAALVPWAYFQYSVPLAGNSLISNS
                     NLISKVYFPRAIIPASSTLSGIVDFAVASVMLVALIAYYRLPLTIEMLMWPVLLVPMI
                     IFTTAVGMLFAALNVKFRDIKYTIPFFIQSMMFLSPVFYPPSIVPEKWAFLLHVNPLV
                     GILDSFRASILPGRTIDWASLGVASAVSVVLFALAAVYFYRTERTFADLI"
     gene            10769..>11669
                     /locus_tag="VEC56_08795"
     CDS             10769..>11669
                     /locus_tag="VEC56_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14292.1"
                     /translation="MREAIHVDHLTKRYRLGALRHETMLREALINALRPGRKKDDEQA
                     ILALNDVSFKVDEGEVVGIIGRNGAGKSTLLKLLSKITYPTSGRIHVRGRVASLIEVG
                     TGFHDELTGRENIYLNGSILGMRKKEIDQRLDQIIDFAGVAKFVDTPIKRYSSGMRLR
                     LGFAVAAHMDPDVLFVDEVLAVGDADFQKKCLNAMDELRSGGRTVLFVSHNMAAIENL
                     CPRTIWIDAGKLREDAASDRVIAAYLSTFSDAQRSGYDLADVENRGGNGMIRFTRVEF
                     LDENGNPAGVFRCGEPMRVRFHYH"
BASE COUNT         1886 a         3941 c         3854 g         1988 t
ORIGIN      
        1 gacggtcggg ttcgccctgt ggtcgttcgg ggtctggtca atggtcgcgc accacgaagt
       61 gtccgcgcac gtcggctcgc gctcgttccg aaagacgctg ccgagcgtgg tcgtgatcgg
      121 cgtggtgttc gtgggcgtca ccgtggccct gggccgcgga ctggcgacgc tgaccgacac
      181 tctcccgccg ggcatgatca cgcgcggggc gacgttggcg gtcatcgcgg tcacggccgg
      241 cgtgcaggcc cttctcgtgt tcgcaccggt cgcgctgcgg ctgcgcggcg gagggccctt
      301 cgccgcgatc cgaagcagcg cgcgttacgc gcttcgacgc ttcggggcca ccgcgatgct
      361 ggtggccacc gtactcctcg tgcacctgcc cctggacggg ctcatcgcaa acaccgacgt
      421 catcgccgcg cgcttccacc ccgaggccgt gttccagctc atgctcgcgt cggtggtact
      481 ggaagtgatc accgccttcg tgctgttcgc gggaaccgtg ggcctggcgc tgcccgagga
      541 aggcggcatg cgatgacgcg cgccaccttg actctgctgg cgttcgtgac cctcctgtct
      601 tcgtgcgcga gccacgacga tcgggtcgcg cgatatcgct tggagcagaa gctctggcgc
      661 gcgcagttct accagcggcg catcaacatc tcggtggtgc gcgcgtcgca aaacgacacc
      721 cgcatggcca tcgccgcctt caacgcggtc ctcgccgacg acccgctcgc gcagcccgcg
      781 gccgcgacgt ggcgccccga cgtggtggcc gacattgcgc agatccacct cgcctcgcgc
      841 gtcgcgctgg ccaacctcta cttcttgagc gagcgctacg cggacgcggg gaagatgtac
      901 gagcgcacgc tcgaagcggc ggggcagggc ctgggcggca gcctcgaggc gcgtttgggc
      961 gccgcgcgct cgatgtacct ggcgggcgaa accaccgaag tgatgcgcca gtgcgcggag
     1021 atgttccggc aaatctcgac ctcggaggag ttctggtcgg ggcgcttcgt ccccgacggc
     1081 gtgttcgtcg acatcccggt ggtgctcgcg cgcatgtacc gcgagacggg cgacggcgcc
     1141 gcgtacgccg aatatcgcgc gctggccggc gagttctacg gtcgcgtcgc cgccacctgg
     1201 ccgggcacgc cgatcgcgtg gcaggcgacc ctgggcgaga ttcaattgcg cctcgtggac
     1261 cacgactggc gcggcgccgc cggccaaatc gaggcgatcc tggcggcgcc caacccgcaa
     1321 cgagacctgg aagggctgcg gctcctgctc ggggagatct atgccttcgc gctcggcgag
     1381 ccggcgcggg cggacgcgat cctcgccgac gtggaaagcc gcgccgcggg gaccgccgcc
     1441 gcctacgccg cccgctacga ccgcggcgcg ctcatgctcg agaccggcga cgaagacggt
     1501 gcgatggcgg tgttccgcgc gctggagcac gacaaggcga cgcccggcga cgtcgccgca
     1561 cgggcgatgt tcgggcgcgc gaccatcctc gagcgccgcg gcgcctggga cgaagcgctg
     1621 caactgttgc ggcgcatgca gcagctctac ccccacgcgc ccgcctccat cgaggcaccc
     1681 atcctcgtca cccgccacta cgtcgccacc ggcgaacatg cgctcgccga gcgcacgctg
     1741 gagcgcgcgc gcgagtacta tctctcgctc atcgaccgca acagccccta tcccggcgat
     1801 cgactgggcg tgcaggacgc gctcgcggcg agctacgcga ccgccgggcg cgccgcggag
     1861 ggtgcggcgc tcctggggtc gggcccgtcg gcgtgggacg acgccagcac cgcggcgggt
     1921 ctgctcaaga gcgcggacgt ctacgcgacc gtcctcggcg actccgcgcg cgcgagcgaa
     1981 atgttgaaga aatgtattga gcgatttccg gaaacacgat attcgagggt agccgcgcgc
     2041 cggctggacg agatcgagca cgggcgcggg gcttccgaag agcgtcccct ggaagaatga
     2101 ctcacgcggg ttcgatgcgt ggatgggaag cagcgtgtga gttccgttac agcgccccac
     2161 ccgttgttct aggcacctta cgaggcacga cccgcaatgg tgccgtgcag attcgaacgc
     2221 gatctggaat tcggcgaggt ctccgcggag ttggcggtga cgctcccgtg gcacgccgcg
     2281 gcattctggg gcatgcccca gcgcattgtg gcctggcgac gcctctgcga gcggtgttgg
     2341 cctcgctatt gctcgttgtg cagggaatcc aaacgacctt caatccgcgc gctgaacgac
     2401 gcggcaggaa tcggtggcat gagacgtaac cgaccgaact ggcgctctgc gaccatcctc
     2461 ggcctggcct tgatcggcct gtcggggccg tttgcggacg tgcgcgccca gcagggcgcc
     2521 ggcacccatg ccgcgccctt tctgaagatc ccggtcggcg cccgcttgat gtcctctccg
     2581 gacgcggtgg cggggctctc gccggacgcc accctcatgt actccaatcc ggccttcctg
     2641 tcgggcctgc cccgggcgga ggccttcatc acgaccagcg aatggctcga caacctgacg
     2701 ttcacctcgg ccggtgtggc aatgcccatc ggagcgcagg ggaccgtcct cggtatcggc
     2761 gcgacgttcc tgtattccgg cggggtccag ggctacgacg ccgccttgaa tccggtctcc
     2821 gaggagagtt tctacgacgt cggcttcgac gtgacggtga gccacgcctt tcgcggcacg
     2881 gggctcgcgc tcgcggcggg gaccaccatc atccgcgagc acgtgctccc gaccgacgga
     2941 tccggctatg ccttccacct cggcgcgtcg tactggatgg gcccgaatct attccacgcc
     3001 gcggcccgtg acctgggcgg cacggtgtcc ttcgactcgg agagctggaa cgtggcgccc
     3061 gagtactcgt tcggcggcgg ccgcatattc gcgtcgagcc tgggccagtt cttcgcgggc
     3121 gcgcagttca cggattcgga cgcatacggg acgcgggtac agctcggcgt cgactatcag
     3181 ttcaacccga tgctgaccct acgcagcggc ttcaccgaca acatggacag cagccagcgc
     3241 ggcgcgccgt tccaggcggg cttcgggctg cactacggcg tcatgacgct cgactacgcg
     3301 tacacgcccc aggagtactt ctcgagcgtg cactcgttct cgctgtcgta cgcgttcggt
     3361 ggtccgtcct cgcagggcgg gcgactgacc gtcccgaccg gcgacttctc gccgcccatc
     3421 gccgactcgg agccggtgcc gccgccgtcg cgcctggcgg ttccgaagga aacctctccg
     3481 tcgcgctccg gtggctccta cctcttgctc gctggatcgc acgcgtggct cgagagcgcg
     3541 cgggccgagg tccgtgcgtt gcagctgctc cagatcccgg cgaagatcga gtcgaacggc
     3601 tcccggtatc gcgtggtcgt cggtcgattc aagtcggcgg acgatgccga gcaggcacgt
     3661 cgcgagtacg cgtcgtccgg tcacgcgttc acggtcctcg ccgaatagcg tcgttcgtcc
     3721 ccccgtcgtc gccgcgcgcc gtgcggtgat ttccccaaga ctcccattgt tcctgcgtca
     3781 ccgggtaact agcagttaaa caataagtta cacgcatcac tgcggtggat aaatgggtga
     3841 attgcccaat gcgccccgat tatcgccata tcgtcgtccc tcgttcgttc gagtcgagtc
     3901 tccgtaaaca cagttaacag catgtgccaa cgctgattac gcgttcgcgc gatcggcgtg
     3961 aggtttgtgc aaagaagcgc atgcgtggca ccaaaaatgc tgaacgcgtt cgcactgcga
     4021 ggtcggggta cgcccccgga gtctccgcga gagactcgtg cgctccgaac ctcgaatcca
     4081 tccacccgaa ctggttgcgc cctgaatgaa cgaattcgcc caaagattcg ctcgccggcc
     4141 ggagatcgtg atcccatgga atcgctcgag cgccgtcgtt cgggttcggc ccgccgaccg
     4201 cgtgcggcat ccctacgacg tcatcgggcg cctggggtgg aagcgcgcgc tggacatcgt
     4261 gctttcgttc gtcggattgg tggtcgcgtc gccggtgatg gtggcggccg ccgtcctcat
     4321 caagaccacg tcgccgggtc cggtgttcta ccgccaggag cggatcggta tcaaccgccg
     4381 gggccgcgac cgccgcggct cgtcgggacg ggccgacatg gaccgccgcc gccgcgatcg
     4441 ccgcgtgctc gtcaacttcg gccggccgtt caccatctac aagttccgca ccatggtcgt
     4501 cgatgccgag cgcggcacac cgcccatgtg ggccaaggaa cgcgaccccc gcatcacgct
     4561 ggtgggacgc gtgctgcgca agacccggat cgacgagctg ccgcagttct ataacgtgct
     4621 gcgcggcgag atgagcatcg tcggtccgcg ccccgagcgc gcctacttca tcgggcgtat
     4681 cgagaaggac ctgccggagt ttcagctgcg cctgcgcacg aagccgggga tcaccggtct
     4741 ggcccaggtc gagttgggct acaccaacac cgacgacggc ctgcgcgaca agctccacct
     4801 cgacctcgag tacattcgcc ggttgagctt ctggaccgac ctcaagatcc tcttccggac
     4861 cgtcttcgtc gtcttcacgg ggaagggcgc ctgctgacgc ccgtccttcg cctcctcccg
     4921 tagtccgaat cgcccgcccc accgcttgct gacgcgggca tgtaacttgc tgacttgaat
     4981 acgtctagcc cttgtccgcc cgttctgctt gcggcggtcc gaaaatcgag tgcatgctct
     5041 tgcgcgtggt cgccaacacg aagattccga tggaattgac gatgcccgtc gaagcctcca
     5101 agcccagggt gagcgtgggg atgcccgtgt acaacgggaa ggcgttcctc gcccagtcca
     5161 tcgagtcggt tctggggcag acctacgcga acctcgagct aatcgtctcc gataacggct
     5221 cgaccgacgg gaccccggag atcgcgcagg actacgcccg gcgggaccgc cgggtccgct
     5281 tcgttcgtac cgaggagaac cgcggcgcgg cctggaacta ccggcgcacc ttcgacctgg
     5341 cgcgcggcga gttctttcgc tgggcgcccg cggacgacct gttcgcgccc gattcgatcg
     5401 cggtgtgtgt ggcggccctc gacgccgacc ccgacgccgt gttgtgctac ccgaagacgg
     5461 agctgatcga ccccgagggg cgcgtgatcc gtgggtacga cgacaacctg gacctgcgcg
     5521 cgagcgatcc ggcggagcgc ctggcgcgcg cgatggccca gatcggtctg gtcaacgtga
     5581 tctacgggct catgcgcacc gcggccctgg gaaagacgcg gctgatgggg accttcgtgg
     5641 gcgcggacga ggtgctggtg ttcgagctgg cgttgcaggg gaagttcctc gagctcccga
     5701 cctcgcgctt ctatcgacgc atccacgaga acgcgttcag ccagatggcg accacggagc
     5761 agaagcaggc gttcttcgac ccgcgcaaga cgggccggtt cagcccgcgc ttgtgggagc
     5821 actaccgtca gtgcctgcac ggcgtcgtcc acgcgcccat ctccgcggcc gccaagtggc
     5881 gcgcgatcgg cgtgctcgtg cgctcggcca tcgccgtgcg cagggacttg atgcgcgaag
     5941 tcgcggccgg ggcgcgacgc gccgcgcgac cgaagttgtt tggcctgtaa accgcgacca
     6001 cggaggattc tcttggtgac tcgaatcgac ggagcccgcg tggagcccct ctccgacctg
     6061 ctgaatcgcg acctcgacta cgtgacgacg gggctgcgcg acgagctggc cgcgatggcg
     6121 gggtcgcgcg tgttgatcac cggcggagcc ggcttcctcg ggtactacat ggtccagggc
     6181 atccttcact ggaacgaccg cgcgaacgaa aaggaccgca tccgcgtctc cgtgttcgac
     6241 aattacgtgc gcggcgtgcc ggcctggctg gaggcgctgc gcggccgcgc cgacctgacg
     6301 ctcacgcgct tcgacgtacg cgaaccgctg ccggacccca tgcccgagtt cgagtacatc
     6361 atccacgcgg ccggcatcgc gtcgcccatc tactatcgcc tgcacccgtt ggagacgatg
     6421 gacgccaaca tcgacgggct gcgcaacctg ctcgagtacg cgcgcgcgcg tgtcgagagc
     6481 aagcgggact tccggggctt catcttctac tcgagcagcg agatctacgg cgacccggcg
     6541 cccgaccaga ttcccacgcg cgaagactac cgcggcaacg tctcgtgcac ggggccgcgc
     6601 gcgtgctacg acgaatcgaa gcgctacggc gagaccctgt gcgtggtgtt cgcgcgccac
     6661 tacggcgtgc ccgtcaaaat ggtgcggccg ttcaacaact acggacccgg cttgaagatc
     6721 agcgaccggc gcgtcattcc cgacttcgcg cgcgacgtga tgaatggccg cgacatcgtc
     6781 atgctctcgg acggcaagcc caagcgcacc ttctgctacg tggccgacgc ggtggtggga
     6841 tactacaaag cactcgtgcg cggggggatc ggcgaggcgt acaacgtcgg cgtggatcgc
     6901 cccgagatct cgatgcgcga gctggccgag aaggtggttg cgaccgcgcg cgacctgttc
     6961 gggtacgccg gccgcgtggt gatgcaggcg cccaccgaga gcgactatct ggtcgacaat
     7021 cccaatcggc gctgccccga cctgaccaag tcgcgcgcgc aactcggcta cgacccgacc
     7081 atcctggtcg acgagggcct acggcgctcg ctggtctggt accactacca tcgcgaagcg
     7141 gaggacgcct gatgcgcgtc tccattattg gggcgggcta tgtcggcatc gtgaccggcg
     7201 cgtgcctggc cgagaagggg cacagtgtgg tgtgcgtcga ggtcgacgcc gccaaggtgg
     7261 agcgcattcg gcgcggcgag ccgcccatcc acgaagaggg cctggccgag ctcctgcgcc
     7321 gccacagcgg gaccgcgttt cgcgccacca cctcgctcgc cgaagcggtc gccgagaccg
     7381 atatcacctt gatcgcggtc ggcaccccct tcgacggacg cgcgatcgac ttgacctatg
     7441 tcacgcgcgc ggcccgcgag atcggcgaat tactcgcgcg caagcgcgag cgacacacgg
     7501 tggtggtcaa gagcaccgtg gtccccggca caacggacgg gccggttcgc gaggccctgg
     7561 agtccgcatc ggggaagaag gccggcgagg acttcgggct gggcgccaac ccggaattcc
     7621 tgaccgaagg aaccgccgtc gccgacttca tgagccccga ccgcatcgtg atcggcggaa
     7681 tcgatgccag aacactggac gcgctgtcgc aactgtatgc cggcttcgag ggcgtgccgc
     7741 tcattcgcac caacaatgcg accgcggaga tgatcaagta cgcgtccaat tcgctgctcg
     7801 cggccatgat ctcgttctcg aacgagatcg ccgacctgtg cagcgccatc ggcggcatcg
     7861 acgccaagga cgtcatggcg ggcgtgcacg agtccgcgta cctcaacacg gtttcgggcc
     7921 acgcgcgggc gcgcgccccc atcgcgagct tcctggaagc gggctgcggc ttcgggggga
     7981 gctgcctgcc caaggacgtg agcgcgctgg tcgcacatgg cgggtcgaag ggggtcgaca
     8041 tgcgcgtctt gcgctcggtg ctcgaggtca accgcgggcg ccccgatcgc gtgatcgagg
     8101 gcctcaagcg acgcgtgccg tcgctccagg gcgccgcagt cgcggtcctg ggcctggcct
     8161 tcaagcccga caccgacgac gtgcgggaat cgcccgcgtt tcccattatc gaccgcctgc
     8221 tcgcggaagg ggcggcggtg accgcctacg acccggtggc caccgagccg gcccggcgcg
     8281 cgctcgccgg caagccggtg cgctacgccc agaccttgga aggggccctc cgcgaggccg
     8341 acgtggtggt cttggtgacc cggtggcgcg agttcgtcgc ggtcccggaa ttgcttcgaa
     8401 agctggggcg gaccccgctg gtgttcgacg gccggcgcat gctggacaag aacgcgattt
     8461 cccgctacga gggaattgga ctgtaagcct tttttggccg ccaggcgcgg ccgggagtag
     8521 caatgaaggt cgtcttgttt tgcggcgggc agggaatgcg catccgggag tactcggacg
     8581 aaatccccaa gcccatggtg ccgatcggct accggcccat cctctggcac gtgatgaagt
     8641 actacgcgca tttcggccac aaggagttca tcctgtgcct cggctacaag gccgacgtga
     8701 tcaaaaacta cttcctcaac tacgacgaat gcctgtcgaa cgacttcacg ctctccggcg
     8761 caggaaaggt ggagctcgcc aacagcgaca tccacgactg gaagatcacc ttcgtcgaca
     8821 ccggtgttaa caccaacatc ggcggccgac tgcaggcggt gcgcaagtac gtcgagaacg
     8881 acgacgtgtt cctggccaac tacagcgaca acctcaccga cctcccgctg ccggagatga
     8941 tccgcgacgt cgggtcgagc ccgcgcgtcg cgaccttcct ggccgtgcgc ccgagccaga
     9001 gcttccacat ggtgtccttc gacgacaagc gctcggtgga cgccatcacg cacgtctcgg
     9061 cgtcggaggt gttcatcaac ggcgggtact tcgtgctgcg caaggagatc ttcgattaca
     9121 tgaagaacgg cgaggagctg gtcgagaagc ccttcgaacg cctgatcgcg aagaaactgc
     9181 tctcggccta ccgttacgag gggttcttcg tcagcatgga cacgttcaag gagcggcaaa
     9241 cgctggagag catgtacacg cgcggcgaag ccccgtggga gctgtgggcc gggaagcggc
     9301 gttaggccac catgaagacc ctcgctgtgg gaagcatcgg ttccatcctg tgcctgggtg
     9361 cgcacagcga cgacattgag atcggctgcg gcggcaccgt gctcaaactc gtggagtcca
     9421 acccgggcct ttccgtccat tgggtggtgt tcagcgccac cggcgagcgt ggcggcgaag
     9481 cgcgcgcttc cgcggaccgc tttctcgcgc gggccggcag gaagaccgtc gagctgcacc
     9541 agtttcgcga cggcttcttt ccgagcgagc actccaagat caagacctac ttcgaagaat
     9601 tgaagtcgcg cgtacagccc gacgccgtgt tcacacacgc gcgcgccgac ctccaccaag
     9661 accaccgcgc gatctgtgag ctgacctgga acacgttccg cgaccacctc gtgctcgagt
     9721 acgaggtgcc caagtgggac ggcgatctgg gcgctcccaa cgtgtacgtc cccctcgacg
     9781 agcgccacgc gaacgaaaag atcgcgatct tgctggagtg cttcgcctcc cagcgcggaa
     9841 agcattggtt cgaccaggag acctttgcgg ggctcatgcg tctgcgcgcg atggagtgca
     9901 acgcgccctc gcggcgcgca gaagcgttct acggccgcaa ggtggtgcta tgagcgccgt
     9961 cgcgcgtccg gtggcggtcg agtggctcta cgagttcgtc cgctaccgcg agctgttctt
    10021 cttcttggtg tggcgcgacg tcaagatccg gtacaagcaa accattctgg gcgcggcgtg
    10081 ggccatcatc cagcccttcg ccctgatggt ggtcgccacc ttcgcgttcg gccgcgccgc
    10141 cggcatcgac agcgaggggg cgccgtaccc cgtgttcgcc tacgccgcgc tggtgccgtg
    10201 ggcctatttc cagtactcgg tgccgctggc gggcaatagc ctcatctcga actcgaacct
    10261 catcagcaag gtgtactttc cgcgcgccat cattcccgcc tcgtcgacct tgagcggcat
    10321 cgtcgacttc gcggtggcgt ccgtaatgct ggtggccctg atcgcgtatt accggctccc
    10381 gctcaccatc gagatgctga tgtggccggt gctgctcgtg ccgatgatca tcttcacgac
    10441 tgcggtcggg atgctgttcg cggccctgaa cgtcaagttt cgcgacatca aatacacgat
    10501 tccgttcttc atccagtcga tgatgtttct ctcgcccgtg ttctatccgc ccagcatcgt
    10561 cccggagaag tgggcgttcc tgctgcacgt gaacccgttg gtcgggattc tcgactcgtt
    10621 ccgcgccagc atcctgcccg ggcggaccat cgactgggcg agcctggggg tggcgagcgc
    10681 ggttagcgtc gtgttgttcg cgctggccgc cgtgtatttc tatcgcaccg agcgaacctt
    10741 cgccgatttg atttaaggag taccggactt gcgtgaagcc atccacgtcg accacctgac
    10801 caagcgctac cgtctgggtg ccttgcgcca cgaaaccatg ctgcgcgagg cgctgatcaa
    10861 cgcgctgcgg cccgggcgca agaaggacga cgagcaggcc atcctggccc tcaacgacgt
    10921 gagcttcaaa gtcgacgagg gggaagtagt gggcatcatc ggtcgcaacg gggcggggaa
    10981 gagcacgctc ttgaagctgc tctccaagat cacgtatccc acctcgggac gcatccacgt
    11041 gcgcggccgg gtggcctcgt tgatcgaggt ggggaccgga ttccacgacg agctcaccgg
    11101 acgcgagaac atctatctca acgggtcgat cctcggcatg cgcaagaagg agatcgacca
    11161 gcgcctggac caaatcatcg acttcgcggg cgtggcgaaa ttcgtcgaca cgcccatcaa
    11221 gcgctattcg tcgggcatgc ggctgcggct gggcttcgcg gtggcggccc acatggaccc
    11281 cgacgtgctc ttcgtcgacg aagtgctcgc ggtgggcgac gccgacttcc agaagaagtg
    11341 cctgaacgcg atggacgaac tgcgctcggg cggccgcacg gtcctgttcg tctcccacaa
    11401 catggccgcg atcgagaatc tctgcccgcg caccatctgg atcgacgccg gcaagctgcg
    11461 cgaggacgcg gcgtcggacc gggtgatcgc cgcgtacctg tccaccttct ccgacgcgca
    11521 gcgctcgggc tacgacctcg ccgacgtcga gaaccgcggc ggcaacggga tgatccgttt
    11581 tacgcgagtc gagttcctcg acgagaacgg caaccccgcc ggcgtgttcc gctgcggcga
    11641 gcccatgcgc gtgcgcttcc actaccacg
//
LOCUS       DASPGK010000311         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_78555, whole genome shotgun sequence.
ACCESSION   DASPGK010000311 DASPGK010000000
VERSION     DASPGK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78555"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..2076)
                     /locus_tag="VEC56_08800"
     CDS             complement(37..2076)
                     /locus_tag="VEC56_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXV14293.1"
                     /translation="MIDAITFEVLRHRLATIVDEGAAVMRNVSGSPSVAQSNDCNVAL
                     LTTAGEGVVIGRTIASHAMSCIQTAKYVLKEYRENPGINPGDMFFTNHPYIGTPHQTC
                     GVMVAPVFAGERIIAWSGAGIHFGDVGGTTPGQVSIGARSIWDEAIPLPPVKIVERGV
                     MRRDIEDDFLTRSRTRVQNAVDLKAMVGAHHAMCRRIESLAQRYGADVVVEALDRVIA
                     TAELKLRSILREVPDGEWSCENFIDYYDAGKLKVYACRLTMTKRGDGLVFDFTRSSKQ
                     AAAVVNATFAATEGYLLRAMLGMFGFAVTHCPAAIQRVVTIEATPGTIVNCAWPAGVC
                     KGTTSVTQSVWQVATHCLSQMLMASEKHRDRAVAVSRGNLTILDLMGTDQYGGPFAAV
                     LQECCLAAGSAAGEVSDGIDTGGVTEPEVSIPNVENNEFRYPVLYLHRRQRRDSGGPG
                     RFRGGVGLSMAFTPHDVDEIPNVMIHTHGVSAPSALGLAGGYPAVLNHLAIKRRTNLE
                     EFFARGAMPTELSELQAEKLELPPPFTYTRLKKGDVFECAGGGGGGYGDPLERAVSAV
                     ERDVVNGVVSREAAATHYGVVIGDNTATEARRTALRKARQETARVVGQALAVAVRCRC
                     GQAPRSLSARDTKYEAEAYTEEQRVRTSSYLINEMFCSACWAVVETRISVPDVEA"
     gene            complement(2069..>2526)
                     /locus_tag="VEC56_08805"
     CDS             complement(2069..>2526)
                     /locus_tag="VEC56_08805"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXV14294.1"
                     /translation="SRLFEMRFRHQVHQVKVPAPGGRLEKREVAELVERFIALYEQSF
                     GKGTAAREAGVEILTFHVVATTHRVPLNLAPAKIEDADASAARSGSRRVWFGGAFVET
                     PIYEHRKLRPGNRVSGPAVIEGLNTTLPLHPGQELSVDAFQNLVIVFND"
BASE COUNT          413 a          851 c          818 g          444 t
ORIGIN      
        1 cgaaggtccg cgtaacgccc tggagggaga tgaagctcac gcctcgacgt caggcaccga
       61 gatgcgcgtt tcgaccaccg cccagcacgc cgagcaaaac atctcgttga taaggtacga
      121 cgaagtgcgt acgcgttgct cctctgtata agcctccgcc tcgtatttgg tatcgcgcgc
      181 cgaaagcgag cgtggcgctt gaccgcagcg gcagcgtacc gcgacggcga gcgcctggcc
      241 gactacgcgc gccgtctcct gacgcgcctt gcgcagcgcg gtgcggcgcg cctccgtagc
      301 ggtgttgtcc ccgatcacta cgccatagtg cgtggccgcg gcttcacgcg aaaccacccc
      361 gttgaccacg tcgcgctcga cagcgctgac cgcgcgttcg aggggatcgc cgtagccgcc
      421 cccgccaccg cccgcgcact cgaacacgtc gcccttcttg agccgcgtat aagtgaacgg
      481 cggcggcagc tcgagcttct ccgcctggag ttcggacagc tcggtcggca tcgcgccgcg
      541 cgcgaagaac tcttctagat tcgtcctgcg cttgattgcg aggtggttca gcaccgcagg
      601 atagccgccg gcgagcccga gcgccgaagg cgcgctgacc ccgtgtgtat ggatcatgac
      661 gttcgggatc tcgtcgacgt cgtgcggcgt gaacgccatg ctcaagccca ccccgccgcg
      721 gaatctgcct gggccgccac tgtcgcgccg ttgccggcga tgaagataga gcacgggata
      781 acggaactcg ttgttttcga cgttggggat gctgacctcc ggctcagtca cgccgccggt
      841 gtcaatgccg tccgacacct cgcccgccgc actgcccgcc gcaaggcagc attcctgcag
      901 caccgcggcg aaggggccgc cgtactggtc ggtgcccatg agatctagga tggtcaggtt
      961 gccgcgcgat acggcgaccg cgcggtcgcg gtgcttctcg cttgccatga gcatctggct
     1021 gaggcaatgc gtcgcgacct gccacacgct ttgcgtgacc gacgtagtac ccttgcacac
     1081 gccggctggc cacgcgcagt tcacgatcgt gcccggcgtc gcttcgatcg tgaccacgcg
     1141 ttggatggcg gccgggcaat gcgtaacggc gaagccgaac atgcccagca ttgcgcgcag
     1201 gaggtatccc tccgtcgcgg cgaacgtcgc gttcaccacg gcagctgcct gcttcgacga
     1261 tcgggtgaaa tcgaacacca ggccgtcgcc tcttttcgtc attgtcagcc ggcaggcata
     1321 gaccttgagc ttgcccgcgt cgtagtagtc gatgaagttc tcgcaactcc actcgccgtc
     1381 gggcacttcg cgtaggatgg accgcagttt gagctcggcg gtggcgatca cgcgatccag
     1441 cgcctcgacg acgacatcgg caccgtagcg ctgtgcgagc gactcgatgc gtcggcacat
     1501 cgcgtggtgc gcgccgacca tagccttgag gtcgaccgcg ttctgcacgc gggtgcgcga
     1561 gcgcgtgagg aagtcgtcct cgatgtcgcg ccgcatgaca ccgcgctcga cgattttcac
     1621 cggtggcagc gggatcgcct cgtcccagat cgagcgcgcg ccaatcgaca cctgccccgg
     1681 ggtagttcca ccgacgtcgc cgaaatggat gccggcgccc gaccaggcga tgatgcgctc
     1741 gccggcgaac accggcgcca ccatgacgcc gcaggtctga tggggcgtgc cgatgtacgg
     1801 gtggttggtg aagaacatgt cgcccgggtt gatgcccggg ttttcgcggt actccttaag
     1861 cacgtacttc gcggtctgga tgcacgacat ggcatgactc gcgatggtgc ggccgataac
     1921 cacgccctcg cccgcggtcg tcagcagcgc cacgttgcag tcgtttgatt gcgcgacgct
     1981 cggcgagccg gaaacgttgc gcatgaccgc ggccccctcg tcgacgatgg tcgcgagccg
     2041 gtgccgcagc acttcgaagg ttatcgcgtc aatcattgaa gacaatgact aggttctgga
     2101 acgcgtcgac gctgagctcc tggcccgggt gcagggggag ggtggtgttc aggccctcta
     2161 tgaccgcggg acccgagacg cggttgccgg gcctcagctt cctatgctcg tagatcggcg
     2221 tctcgacgaa cgcgccgccg aaccagacgc ggcgcgaccc cgagcgcgcc gcggaggcat
     2281 ccgcgtcttc tatctttgcc ggcgccaggt tgagcggcac gcgatgcgtg gtggccacga
     2341 catgaaacgt gaggatctcg acgcctgcct cccgggcggc cgtccctttg ccgaagctct
     2401 gctcgtacag ggcgatgaac cgctccacca actcggcgac ctcgcgcttt tcgagcctgc
     2461 cgcccggtgc cggcaccttc acttggtgca cctggtggcg gaagcgcatc tcgaacaaac
     2521 gcgaga
//
LOCUS       DASPGK010000312         8427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_195299, whole genome shotgun sequence.
ACCESSION   DASPGK010000312 DASPGK010000000
VERSION     DASPGK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195299"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VEC56_08810"
     CDS             <1..343
                     /locus_tag="VEC56_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14295.1"
                     /translation="AEPNAMTFPALELATTVFLCPRFFDDDWMSLHGTRDPLGARAWD
                     LVHEAVHLAGVDDQHETYSLDRSRCGSGSDGLHPSFALINADTYACLIYSIGRGEAVR
                     PALPPLVLPEV"
     gene            417..506
                     /locus_tag="VEC56_08815"
     tRNA            417..506
                     /locus_tag="VEC56_08815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Ser,seq:cga)
     gene            557..632
                     /locus_tag="VEC56_08820"
     tRNA            557..632
                     /locus_tag="VEC56_08820"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:590..592,aa:Ala,seq:cgc)
     gene            700..799
                     /gene="ffs"
                     /locus_tag="VEC56_08825"
     ncRNA           700..799
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEC56_08825"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            866..2641
                     /gene="dnaX"
                     /locus_tag="VEC56_08830"
     CDS             866..2641
                     /gene="dnaX"
                     /locus_tag="VEC56_08830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXV14296.1"
                     /translation="MSYEVLARKWRPLTFDDVVGQEHVTHTLKKAVETARVSHAYLFS
                     GPRGCGKTSTARILARVLNCENVQGGNPCNACPSCLAVIKGNHLDVMEIDGASHTGVG
                     EVRELQESIGFAPSQSRNKVYVIDEVHMLSTHAFNALLKTLEEPPAHVYFIFATTSPQ
                     KIPDTIKSRCQRHHFKRLENAEIAARLEDICKAEKVKYEMDGLRLLARKADGSMRDGT
                     SLLDQCVTASGGDVSAEAVRGILGLVDQEKVLAFVDAIAAGDATRPLQLLDAAVNEGV
                     DLQDLLAALIESYRDLMVLSVPGDLSSLLFRSQSEIEHLNSSRAHYELADLVTMVERL
                     CNAAPRLKTMSDPRVYLETVLVDLTLLDRQTDIRTLISRLEGSSVEGSGETRGGRNAA
                     PASPRKASDAREAAGDPGARAGSASLLATGDLGANPRQPASPPVAPVIDAGEREEADA
                     SPVPNTEGPFDLAKIQQLWDGFVTFVRQKKVSLGVCLISGKPYAFDGKRLLVRFMKSF
                     ALQREQAARPESIEFVRAMFERYFGHAVEIVCFSEGEEKAMALEDALQKKPPEERFRD
                     IPEDKKPIMQRLINDFDGEIIRYQT"
     gene            2668..2976
                     /locus_tag="VEC56_08835"
     CDS             2668..2976
                     /locus_tag="VEC56_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXV14297.1"
                     /translation="MNMMKMMKQAQEMQAKMARLEDDLAREEFEVSSGGGAVTVRMNG
                     KQVVLKISIREDVVKDGDKEMLEDLITAAVNEAHERASGLAKERIAAITGGLKIPGLF
                     "
     gene            2992..3606
                     /gene="recR"
                     /locus_tag="VEC56_08840"
     CDS             2992..3606
                     /gene="recR"
                     /locus_tag="VEC56_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXV14298.1"
                     /translation="MGFPPADFGSETLSALVKSLSRLPGIGSKSALRLALYLLRPDNT
                     DADRISHLLAELKSKVRFCKRCGAITEAEECAICTDPGRAQDTICVVAAPEDVIVMER
                     TKHYRGLYHVLHGVLSPVDGVGPDDLTLRALEARVRGGKVRELIVATNPTVEGDATAL
                     YLAKTVAPYGCTVTRLARGLPMGGTLEFVDDTTLARAIDRRETL"
     gene            3676..4173
                     /locus_tag="VEC56_08845"
     CDS             3676..4173
                     /locus_tag="VEC56_08845"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HXV14299.1"
                     /translation="MVRADWTIYEEDYWAINSVIKELIENSNSTSVILIDKTGQLIAS
                     VGMEPEFDLMSFASLCAADFEANAQLAKLIGEKDFSTLYHQGVDESMYLARVAQHIIL
                     VVLFNKRTTLGLVRLRVKKAVDSLDTVLNRLFDKLEYENEDLKQFDAGFTEELNKEID
                     SLFKD"
     gene            4200..4784
                     /locus_tag="VEC56_08850"
     CDS             4200..4784
                     /locus_tag="VEC56_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HXV14300.1"
                     /translation="MSLINYSSREINCKIVYYGPGLCGKTTNIQYVYNKVDPSTKGKL
                     ITLATEMDRTLFFDFLPLELGQVKGFKTRFHLYTVPGQVYYDASRKLILRGVDGIVFV
                     ADSQSARYDANIESLYNLHENLEEYKLKLDDVPFVLQYNKRDMDNIIPMEEMEQELNP
                     EKFPSFPAVAVKGDGVFDTLKCVAKGVLKKLSNN"
     gene            4877..5527
                     /locus_tag="VEC56_08855"
     CDS             4877..5527
                     /locus_tag="VEC56_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14301.1"
                     /translation="MRTPVFTRLLPFVFLLLVLSCGTRSRMLNMWVDPDFPPGPMTKV
                     LVLALWENRAARETWERAFVHALEKNDVVAVPSHHLLPAAMPDSVTVFDAARRAGCDG
                     VIAIYETYEHTKTVYVPGYVVREVQVISGRGYEVFAHRAYERVRYTETYVPSHERIQT
                     AVRCDVEVWSAPDDVRMVWSGTTEVIEPDSDWSTCTVVAGWVEYQLIFCGLVPTDL"
     gene            complement(5547..6899)
                     /locus_tag="VEC56_08860"
     CDS             complement(5547..6899)
                     /locus_tag="VEC56_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HXV14302.1"
                     /translation="MKTIIEPFRIKVVEPIRMTTRGEREAILRKADYNLFRIHADDVL
                     IDLLTDSGTGAMSSLQWSGVMRGDESYAGAKSWYVLEQSVRALTGFEHILPTHQGRAS
                     ERILFELVGGPGQVVPNNSHFDTTRANIEHSGARAVDLLIPEGRDPRSRHPFKGNMDV
                     AALERLIGEVGSDHIPLVMLTVTNNSGGGQPVSLENIRAVRAACDRHRLPLFLDACRF
                     AENAYFIKVREAGQAGRSVAEIAREMFDLCDGATISAKKDGLVNIGGLLLLRDPKLVA
                     RADELLILTEGYLTYGGLAGRDLEAMAQGFREVVDEDYLHYRIRSTEYLGERLLRAGI
                     QIVEPPGGHAIYIDAAAFAPHIPPERFPGQALAVAMYRHAGIRSCEIGSVMFGRSGRP
                     PMELVRLAIPRRVYTQSHIDYVVEALTEISADRGALRGLRIVEEPTSLRHFTARFEEI
                     "
     gene            complement(6896..8296)
                     /locus_tag="VEC56_08865"
     CDS             complement(6896..8296)
                     /locus_tag="VEC56_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HXV14303.1"
                     /translation="MENRRSWAEPWKVKMVEHLRMTTRAERERAIAEAGFNTFLLPSQ
                     FVYIDLLTDSGTSAMSDTQWAGLLKGDEAYAGSMNFYHLETAVRRFYGYKHLIPTHQG
                     RAAEHIMSQLCIRAGQVVPGNMYFTTTKVHQELAGGKFVDVIVDEAHDPESDFPFKGN
                     VDLGKLERLIREVGAKGIAYLSLETNVNMAGGQPCSLANIRDTCALCHRHGIPVYLDA
                     TRAVENAYFIRAREPGQAHRTLSEILREICDQTDGCTMSAKKDALVNIGGFLAVNDDD
                     LAERARNMVVIYEGLHTYGGLAGRDMEAMAIGIEESLQLDHMHARIGQVEYLGERLLR
                     AGIPIVKPIGGHGVFLNAAKFLPHIPRDQFPAQALAAALYVDSGVRSMERGGVSAGRD
                     ASGANLFPALELVRLTIPRRVYTQAHMDVTAESVIALYENRNQIVGLRFTHEPKHLRF
                     FQARFAPVEGNVVLRA"
BASE COUNT         1485 a         2736 c         2757 g         1449 t
ORIGIN      
        1 ggcggagccc aacgcgatga cgttcccggc gctcgagctc gccacgacgg tgttcctgtg
       61 tccgcgcttc ttcgacgatg attggatgtc cctgcacggc acgcgcgatc ccctcggggc
      121 gagggcgtgg gacttggtcc acgaggcggt gcacctggcc ggtgtggatg atcagcacga
      181 gacctactcc ctcgatcgct cgcgttgcgg ctcagggtcc gatgggctgc atccgtcgtt
      241 cgcgctgatc aatgcggaca catacgcgtg cctgatctac tcgatcggcc ggggggaggc
      301 ggtcaggcca gctctccctc cgctcgtcct gcctgaggtg tgaccggccc gcgtgaaaga
      361 gatgagccgc ggcggagacc ttgcctttcc gggcgatggt gggttagtat tcgcccggag
      421 agatgccaga gtggttgaat ggggcggtct cgaaaaccgt tgagcgcgca agcgttccga
      481 gggttcgaat ccctctctct ccgccatatt tcgtgacctc gacataacac ccgaccgcgc
      541 ggaaacagac cagggtgggg ctgtagctca gctgggagag cgcctcgttc gcaatgagga
      601 ggtcgtcggt tcgatcccga tcagctccac cattttcttt ccgcgcggcc gatgtggaac
      661 gccggccgcg gcccatcccg ggtcgcggtc gcgagtggcg gccgggtccc gcgcaatgat
      721 ccttcgtgaa tcccgtcagg accggaaggt agcagcggta agcggcccgg atcatgtgac
      781 gcggtcaagc ctggccgcca ctcgcgacgc cattccgaac cactaactcg gtcgcgcggt
      841 gcgcgacccg acgggacagc gcgcaatgag ctacgaagtc ctcgcacgca agtggcgccc
      901 gctcaccttc gacgacgtgg tcgggcagga gcacgtcacc cacaccctca agaaggccgt
      961 cgaaaccgcg cgtgtctcgc acgcctacct cttctcgggc ccgcgcggct gcggcaagac
     1021 cagcaccgcg cgtatcctgg cgcgcgtgtt gaactgcgag aacgtccaag gcggcaatcc
     1081 gtgcaacgcg tgcccatcgt gcctcgccgt catcaagggc aaccacctcg atgtgatgga
     1141 gatcgacggc gcgtcgcaca ccggcgtggg cgaagtccgc gagttgcagg aaagcatcgg
     1201 cttcgcgccc agccagagcc gcaacaaggt ctacgtcatc gacgaagtcc acatgctctc
     1261 gacgcacgcc ttcaacgcgc tcttgaagac gctcgaagag ccccccgcgc acgtgtactt
     1321 catcttcgcg accacgtcgc cgcagaagat ccccgacacc atcaagtcgc gctgccagcg
     1381 ccaccacttc aagcggctcg agaacgccga gatcgccgcg cgcctggagg atatctgcaa
     1441 ggcggagaag gtgaagtatg agatggacgg gctgcgcctg ctcgcgcgca aggcggacgg
     1501 cagcatgcgc gacggcacca gtctcctcga ccagtgcgtc accgcgtcgg gcggcgacgt
     1561 gagcgccgag gcggtgcgcg gcatcctcgg cctggtcgac caggagaagg tgctcgcgtt
     1621 cgtggacgcg atcgccgccg gcgacgcgac ccggccgctc cagctcctcg acgccgccgt
     1681 caacgaaggc gtcgacctgc aggacttgct cgcggcgctg atcgagagct atcgcgacct
     1741 catggtgctc tcggttccgg gcgatctgtc gtcgctcctg ttccggtcac agagcgagat
     1801 cgagcacttg aactcgagcc gcgcacacta cgaattggcc gacttggtca cgatggtcga
     1861 gcggctgtgc aacgcggccc cgcgcttgaa gacgatgtcg gacccgcgcg tgtatctgga
     1921 gacggtgctg gtcgacttga ccctgctcga ccgccagacc gacattcgca ccctgatctc
     1981 gcgcctcgaa ggatcttccg tcgaaggcag cggtgagacc cgcggcgggc gcaacgccgc
     2041 gcccgcgagc ccgcgcaagg cgagcgacgc gcgcgaggcg gcgggcgacc ccggcgcgcg
     2101 cgcgggctcg gcgtcgctct tggcgaccgg cgacctgggc gcgaatccgc gccagcctgc
     2161 gagtcccccg gtcgctccgg tcatcgacgc gggcgagcgc gaagaggccg acgcgtcgcc
     2221 cgtgcccaac accgagggcc ccttcgacct ggccaagatt cagcagctgt gggacggctt
     2281 cgtcaccttc gtgcgccaga agaaagtatc gctgggcgtg tgcttgatct cgggcaagcc
     2341 ctacgcgttc gacggcaagc gcctgttggt tcgcttcatg aagagcttcg cgctccagcg
     2401 cgagcaggcg gcgcgccccg agagcatcga gttcgtgcgc gcgatgttcg agcgctactt
     2461 cgggcacgcg gtcgagattg tgtgcttctc ggaaggggag gagaaggcga tggcgctcga
     2521 ggacgcgctg cagaagaaac cgcccgagga gcgcttccgc gacattcccg aggacaagaa
     2581 accgatcatg cagcgattga tcaacgactt cgacggcgag atcattcgct atcagacgta
     2641 acgcggcgaa aggaccgaga gacgaccatg aacatgatga agatgatgaa gcaggcccag
     2701 gagatgcagg ccaagatggc ccgtctcgag gacgatctcg cgcgcgagga attcgaggtg
     2761 tccagcggcg ggggagcggt gaccgtgcgc atgaacggca agcaggtggt tttgaagatc
     2821 tcgatccgtg aagacgtcgt caaggacggc gacaaagaga tgctcgagga cctgatcacc
     2881 gcggccgtca acgaagcgca cgagcgcgcg tcgggcctgg ccaaggagcg catcgccgcg
     2941 atcacgggtg gcctcaagat cccgggcttg ttctaaggcg cgcgcgcgtc catgggtttt
     3001 cctcccgcgg atttcggatc ggagacgtta tcggcgctgg tcaagtcgct ctcgcgtctg
     3061 cccggtatcg gcagcaagag tgccttgcgc ctcgcactct atctgcttcg ccccgacaac
     3121 accgacgccg accgcatctc gcacctgctc gccgagctca agtccaaggt tcgcttctgt
     3181 aagcgctgcg gggccatcac cgaggccgag gaatgtgcaa tttgcacgga ccccggccgg
     3241 gcccaggaca ccatctgtgt ggtcgccgcc cccgaggacg tcatcgtcat ggagcggacc
     3301 aaacattacc ggggtctgta ccatgtcctg cacggggtcc tctcaccggt cgacggggtg
     3361 ggtcccgacg acctcacgct gcgcgccctg gaggcccgtg tgcggggcgg aaaagtgcgg
     3421 gaactcattg tggccacgaa tcctaccgtg gagggcgacg ccaccgccct gtacctcgcc
     3481 aagaccgtcg ctccctacgg ctgtactgtc acgagacttg ctagagggct cccaatgggc
     3541 gggacccttg aattcgttga cgatacgacc ctcgcccgcg ccatcgaccg ccgcgaaacg
     3601 ctctagcgac tccccgaccg gacggtccgg tcggcagaga cttcaggcgg cgtcccatcg
     3661 acgttcggag cgactatggt acgtgccgac tggactatct acgaagaaga ctattgggcc
     3721 atcaattcgg tcatcaagga gctgatcgag aactccaact cgaccagcgt catcctgatc
     3781 gacaagaccg gccagctcat cgcgagcgtg ggcatggagc ccgagttcga cctgatgagc
     3841 ttcgcctcgc tgtgcgccgc cgacttcgag gccaacgcgc agctcgcgaa gctgatcggc
     3901 gagaaagact tctcgaccct ctaccaccag ggcgtcgacg agagcatgta tctcgcgcgg
     3961 gtcgcccagc acatcattct ggtggtactc ttcaacaagc gcaccacgct gggactggtg
     4021 cggctgcgcg tcaagaaggc cgtcgacagc ctcgacaccg ttctcaaccg tttgttcgac
     4081 aagctcgagt acgagaacga ggacttgaag cagttcgacg ccggcttcac cgaagagctc
     4141 aataaagaga tcgattcgct gttcaaagac taggcaccga cgcaggggca cgaagcggca
     4201 tgtcgctcat caactactcg tcgcgcgaga tcaactgcaa gatcgtgtat tacggtcccg
     4261 ggttgtgcgg caagacgacg aacatccagt acgtctacaa caaggtcgac ccttcgacga
     4321 agggtaagct catcacgctg gcgaccgaga tggaccgcac gctattcttc gacttccttc
     4381 cgctcgagct cgggcaggtg aagggcttca agacccgctt tcacctctac accgtgcccg
     4441 gccaggtcta ttacgacgcc tcgcgcaagc tcatcctgcg cggtgtcgac ggcatcgtgt
     4501 tcgtcgccga ctcacagtcc gcgcgctacg acgccaacat cgagagcctc tacaacctcc
     4561 acgagaacct cgaggaatac aagctcaagc tcgacgacgt ccccttcgtg ctccagtaca
     4621 acaagcgcga catggacaac atcattccca tggaagagat ggagcaggag ctgaacccgg
     4681 agaagttccc cagcttcccg gcggtcgcgg tcaagggcga cggcgttttc gacaccctca
     4741 agtgcgtggc caagggcgtc ctgaaaaagc tctccaacaa ctagccttcc cagagcgttg
     4801 cgtcccggcg ccgggtgcgc taccatgtag cgcgtcacgc cggtgtctcc tgatactgcc
     4861 ggcggcggag tccatcgtgc gtacgcccgt gttcacgcgc ctgctcccct tcgtctttct
     4921 gcttctcgtt ctttcctgcg gcacccgttc acgcatgctc aatatgtggg tggatcccga
     4981 cttcccgccg ggcccgatga cgaaggtcct cgtcctcgca ttgtgggaga acagggcagc
     5041 gcgtgaaacc tgggagcgtg cgttcgtgca cgcactggag aagaacgacg tggtcgcggt
     5101 gccgtcgcat cacctgctcc cggccgcgat gccggacagc gtgaccgtgt tcgacgccgc
     5161 ccgccgcgcg gggtgcgacg gcgtgatcgc gatctacgag acctacgagc acaccaaaac
     5221 ggtctacgtt ccggggtacg tcgtgcggga agtgcaggtg atctccgggc ggggctacga
     5281 agtgttcgcg caccgcgcat acgaacgcgt gaggtacacc gagacatacg tcccgtcgca
     5341 cgagaggatc cagacggcgg tccgctgcga tgtcgaagtg tggtccgccc ccgacgatgt
     5401 tcgcatggtg tggtcgggaa ccacggaagt gatcgagccc gactccgatt ggagcacctg
     5461 caccgtcgtg gccggttggg tcgagtacca gctgatattc tgcggcctgg ttccgaccga
     5521 tctctgacgg gccccggccc cgaacgctag atctcctcga agcgcgccgt gaagtggcgc
     5581 agcgacgtcg gttcctcgac gatcctcaag ccgcgcaacg cgccccggtc cgcggaaatc
     5641 tccgtcaacg cttcgaccac gtagtcgatg tggctctggg tgtagacgcg ccgcgggatg
     5701 gcgaggcgca ccagttccat cggcgggcgc cccgatcttc cgaacatcac gctcccgatc
     5761 tcgcacgagc gaatgccggc gtggcggtac atcgcgaccg cgagggcctg gccggggaag
     5821 cgctcgggcg ggatgtgggg cgcgaacgcg gccgcgtcga tgtagatcgc gtgaccgccg
     5881 ggcggctcca cgatctggat gccggcgcgc agcaggcgct cgcccaggta ctcggtgctg
     5941 cggatgcgat agtgcaaata gtcctcgtcc acgacttcgc ggaagccctg tgccatcgct
     6001 tccaggtcgc ggcccgccag gccgccgtag gtgagatagc cctcggtcag tatgagaagc
     6061 tcgtcggcgc gcgccaccag tttcggatcg cgcagcaaga gcaacccgcc gatgttgacg
     6121 agcccgtcct tcttcgcact gatggtggcg ccgtcgcaca ggtcgaacat ctcgcgcgcg
     6181 atctcggcga ccgagcgtcc ggcctggccc gcttcgcgca ccttgatgaa gtacgcgttc
     6241 tcggcgaagc ggcaggcgtc caggaacaac ggcaggcggt ggcggtcgca cgccgcgcgc
     6301 acggcgcgaa tgttctcgag actcactggc tggccgccgc cggaattgtt ggtgacggtg
     6361 agcatgacga gcgggatgtg gtcgctcccg acctcgccga tgagccgttc gagggccgcc
     6421 acgtccatgt tgcccttgaa cgggtggcgc gaacgcgggt cgcgtccctc cggaatcaag
     6481 aggtcgaccg cgcgcgcccc cgagtgctcg atgttggcgc gcgtggtgtc gaagtggctg
     6541 ttgttgggaa ccacctggcc cggtcctccc accagctcga acaagatgcg ttcgctcgcg
     6601 cggccctggt gggtgggtag gatgtgctcg aagccggtca gcgcgcgcac ggattgctcg
     6661 agcacgtacc agctcttggc gccggcgtag gactcgtcgc cgcgcatcac ccccgaccac
     6721 tgcaggctgc tcatcgcccc cgtgccgctg tcggtcaata gatcgatcag cacgtcgtcg
     6781 gcgtgaattc gaaacaaatt gtagtcggcc ttgcgcaaga tagcctcgcg ctccccgcgc
     6841 gtggtcatgc ggatgggctc gacgaccttg atccgaaacg gctcgatgat ggtcttcatg
     6901 cgcgcaacac cacgttcccc tcgaccggcg cgaagcgagc ctggaagaag cgcaggtgct
     6961 tgggctcgtg cgtgaagcgc aagccgacga tctggttccg gttctcgtag agcgcgatca
     7021 ccgactccgc ggtcacgtcc atgtgcgcct gggtgtagac gcggcgggga atcgtgagcc
     7081 gcaccagctc gagggcgggg aacaggtttg cgcccgacgc gtcgcgaccg gccgagacgc
     7141 cgccgcgctc catggagcgt acacccgagt ccacgtacaa cgcggccgcc agcgcctgcg
     7201 cggggaattg gtcgcgcggg atgtggggca ggaacttggc cgcgttgagg aacacgccgt
     7261 gcccgccgat cggtttgacg atggggatgc cggcgcgcag cagccgttcg cccaagtact
     7321 ccacctgtcc gatgcgcgcg tgcatgtggt cgagttggag ggactcttcg atcccgatcg
     7381 ccatcgcctc catgtcgcga cccgccaggc ctccgtaggt gtgcaggcct tcgtagatga
     7441 ccaccatgtt gcgcgcgcgc tcggccaggt cgtcgtcgtt gacggccagg aagccgccaa
     7501 tattcacgag cgcgtccttc ttggccgaca tggtacaccc gtcggtctgg tcgcagatct
     7561 cgcgcaggat ctcggagagc gtgcggtgcg cctggcccgg ttcgcgcgcg cgaatgaagt
     7621 acgcgttctc gaccgcgcgc gtggcgtcca ggtagaccgg gatgccgtgg cggtgacaga
     7681 gcgcacaggt gtcgcggatg ttcgcgaggc tgcacggctg cccgccggcc atattgacgt
     7741 tggtctcgag cgaaaggtag gcgatgccct tggcgccgac ctcgcggatc agtcgctcca
     7801 acttgccgag gtcgacgttg cccttgaagg gaaaatcgct ctcggggtcg tgggcctcgt
     7861 cgacgatcac atcgacgaat ttgccgcccg cgagctcctg gtgcaccttg gtggtggtga
     7921 agtacatgtt gcccggaacc acctggcccg cgcggatgca caactggctc atgatgtgct
     7981 cggcggcacg cccctggtgg gtgggaatga ggtgcttata gccgtaaaag cgccgcacgg
     8041 cggtttcgag gtgatagaag ttcatcgagc cggcgtaggc ttcgtcgccc ttgagcaggc
     8101 ccgcccactg ggtgtcgctc atggccgagg tcccggagtc ggtgagcagg tcgatgtaga
     8161 cgaattggct cgggagcagg aaggtgttga aaccggcctc ggcgatggcg cgctcgcgct
     8221 ccgcgcgcgt ggtcatgcgg aggtgctcga ccatcttcac cttccagggc tcggcccagg
     8281 aacgtcggtt ttccatggaa tgtcctcgag ttggaatagc gtgtcgaacc ggcgctctgg
     8341 ccggcgaacg tgcggctcac ccgattagac ggcaccgcgc ggccggcgtc aaggacgggt
     8401 ttggccgctc acttcttctc cgcggac
//
LOCUS       DASPGK010000313         6383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_430308, whole genome shotgun sequence.
ACCESSION   DASPGK010000313 DASPGK010000000
VERSION     DASPGK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430308"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VEC56_08870"
     CDS             <1..495
                     /locus_tag="VEC56_08870"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14304.1"
                     /translation="ARYNLALCHRLLGDVDQARAMLEAYRKENPKDERAAEVAHQLGA
                     LDEESGRYAEAAGEYERALASKVDAALAVELNYRVGVCREKLGDEKSALAAYARASAH
                     GDRANTYRLSALARSAALYETRKDYPKALAAYRDLIKNATDPEIVVAARERASELQSG
                     GAKQ"
     gene            514..1215
                     /locus_tag="VEC56_08875"
     CDS             514..1215
                     /locus_tag="VEC56_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV14305.1"
                     /translation="MFTEFDWLKIASESPVMLVLIACSVVTVGVAIERTYYFWLRRGN
                     PEKTAREVTQRVQRGEIKEALWTCQHSAHPVGAAMEVVVGSPVDRPDDVDEQLQVVLT
                     NQKLLLERNLNVLGTMAAIAPLVGLLGTVWGIMRAFHDMALVGSAAPSVVAAGIAEAL
                     VTTAIGLLVAVPALMFYNHFARRMNVMLIMAENHARALRTTMNNGGGTPGARLKPKDD
                     AAVRSIRDRQPVSVG"
     gene            1219..1635
                     /locus_tag="VEC56_08880"
     CDS             1219..1635
                     /locus_tag="VEC56_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV14306.1"
                     /translation="MGANPNTFNGGASPNHEVNMVPLIDVSLVLVVMLLLATPLAFES
                     RIDVANASRTAKQAEKVDKSERVEITVISEDSVRVNRRIVARAQLGESLTPLLETSAE
                     RGVRVACAPRVSHGAFVDVLDQAKMSGAVDISVIER"
     gene            1638..2066
                     /locus_tag="VEC56_08885"
     CDS             1638..2066
                     /locus_tag="VEC56_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV14307.1"
                     /translation="MKRYVTDTSTPVARINVVPIIDVALVLVIILMVTAPMIAVSSLD
                     VELPRAKTRSMEGVDRVHVTFGTTGALAVDEKDVTAQEFSAALAASMAEHDDDILVVV
                     RADARVSYEAVEALVKEIRSAGAKRVAIGTTQHGGTKSDE"
     gene            2059..3024
                     /locus_tag="VEC56_08890"
     CDS             2059..3024
                     /locus_tag="VEC56_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV14308.1"
                     /translation="MSELLLDREFSRIGGRTRHSIAASAVLHALLFLLLGLYQAVGGD
                     TDGLTEITWVDATVPDGVPDAAPPMADEETKSAPVREVKTVATREAESAEHFERALER
                     GTAAPRPQSSRAVTDILNERISALENNASDSATRLASLVTPPNVGVPAPAGVPASETV
                     GGRATPSTLHRDASPGGSVSAPSALRRVEGAPSRPVMVAIAGAPPVSAVPAAKPEDVS
                     RTRDLAGARIIGPVADRAVVSYQVPDYPEWAKREGIEGSVTLYFFVLPDGHVKENVLV
                     ERTSGFTDFDEGAVKALLAWRFATLPGGSEQWGRITFHYRLGNAN"
     gene            3049..3981
                     /locus_tag="VEC56_08895"
     CDS             3049..3981
                     /locus_tag="VEC56_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14309.1"
                     /translation="MRRWKYSLVGTLASLCVVSATQDASPAAAKSKKAAPDSTMNIKA
                     GSGGKDFESITVEGEDRVRVQFERPPLKIELDPASAPGLDWESVWDVLGAESFDPVEP
                     VLSRPAFERTPFAPRPWLDQFRAGPVARFRPDVSGVEKWALEVADSRGKTVARFAGEG
                     KPPQEIVWNGATIDGTPARADLTYSYVLNASDKAGNKRSFLGDSFQLPPRAMDTRDGI
                     GLSFSVPAETAAGVPRALVLEAASRLNQTGDSSTRVHVEVTAPTFPLAKSIADDVVSA
                     LRPHLLGDPSRVTATTNVDAGGGEKASVTIRTGP"
     gene            complement(3987..4415)
                     /locus_tag="VEC56_08900"
     CDS             complement(3987..4415)
                     /locus_tag="VEC56_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14310.1"
                     /translation="MKAKAKTKAKSTLKTKTTTTSNSEFTAAHKKLEAILRKYRRGLQ
                     SRPDPTGGFCLVGPPTETSRGREVWFGAVQTRKNYVSYHLMPVYAFPDLLEGASPELK
                     RRMQGKSCFNFKGVDAKLFAELAKLTEKGYQRFKEEKLIR"
     gene            complement(4416..5159)
                     /locus_tag="VEC56_08905"
     CDS             complement(4416..5159)
                     /locus_tag="VEC56_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14311.1"
                     /translation="MSHSLFLALGYAVACGLWWATNRFVPLWKDPARPTFARPWREVA
                     LVLLGVVGTLLLGQLWSRGIRLEGSGWLGTVGESINQIVIFAPIMLVPVLRRNGWASA
                     WIQRHALLLRVTTGVAFALVALFLYSQFEAGAPSYADTLRGVFDPSRAHLAVQVLLED
                     VAIAILFVRLAAALGQRKAIILVALLFAGAHIPAMVAQGASAADLLALVRDFGLGLLV
                     LATLWRSADVAWLWPVHYALDMTQFLGGL"
     gene            complement(5161..>6383)
                     /locus_tag="VEC56_08910"
     CDS             complement(5161..>6383)
                     /locus_tag="VEC56_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXV14312.1"
                     /translation="LGASAVATVGLTESILTLIYAVAIGLSMGTTAMVARRIGEKNPR
                     EAADTAVQSIIVGLVASIPVSLVGIFYSKDLLALMGADAWALAEGYKYTQWMLGGNAV
                     IMLIFVINAIFRGAGDAAIAMRVLWVANAINIVLDPALIRGWGPLPEMGIEGAAVATN
                     IGRGVGVLMQFVVLFRGGKHIRVLVSQVHVHAEVMWRVVKVSLGGIGQFIIATSSWIG
                     LVRIMSTFGAEALAGYTIAVRIIIFTFLPAWGLSNAAATLVGQNLGAKQPDRAARSVW
                     ITGVATMAFMLLVSVVYIFGNEMLVRIFTDEAAVVAAGAQCLRIVSYGYLFYAWELVM
                     IQAFNGAGDTITPTKINFFCFWLFEIPFAYLLALKWGAGEQGVYWAIVATESMAGLIA
                     IWLFRRGTWKTRMV"
BASE COUNT         1180 a         2144 c         2100 g          959 t
ORIGIN      
        1 gcccgctaca acctcgctct gtgccatcgc ctgctgggcg acgtcgacca ggcgcgcgcg
       61 atgctcgaag cgtatcgcaa ggagaacccc aaggacgagc gcgcggcgga agtcgcgcac
      121 cagttgggtg ccctcgacga ggaatcgggc cgctacgcgg aagcggccgg cgaatacgag
      181 cgcgctctcg cgtcgaaagt cgacgctgcg ctggcggtgg agctgaacta tcgtgtcggc
      241 gtgtgccggg agaagctcgg cgacgaaaag tccgccctgg ccgcctacgc gcgcgcgagc
      301 gcgcacggcg acagggccaa tacctaccgt ctttccgccc tcgcgcgcag cgccgcgctg
      361 tacgagacgc ggaaagacta cccgaaggcg ctcgccgcct atcgagatct catcaagaac
      421 gcgaccgacc ccgagatcgt cgtcgccgcc cgcgagcgcg cgagcgagct ccaatccggc
      481 ggcgccaaac agtagccaaa aggaggcacg gtgatgttca ccgagttcga ctggttgaaa
      541 attgcgagcg aaagcccggt catgctggtg ctgatcgcct gcagcgtcgt gacggtcggc
      601 gtcgcgatcg agcgtaccta ttacttctgg ctccgccggg gcaacccgga gaagaccgcg
      661 cgcgaggtga cgcagcgcgt gcagcgcggc gagatcaagg aagccctgtg gacctgccag
      721 cactccgccc acccggtggg cgcggccatg gaggtcgtgg tgggttctcc ggtcgaccgt
      781 cccgacgacg tcgacgagca gctccaagtg gtgctcacga accaaaagct cctgctcgag
      841 cggaatctca acgtgctcgg caccatggcc gcgatcgcgc cgctggtggg attgctcggc
      901 accgtatggg gcatcatgcg cgcgttccac gacatggcgc tggtgggctc ggcggctccc
      961 tccgtggtcg cggccggtat cgccgaagcg ttggtaacga cggccatcgg actcctggtc
     1021 gcggtgccgg cactcatgtt ctacaaccac ttcgcgcgcc gcatgaacgt catgctaatc
     1081 atggccgaga atcacgcccg cgcgctgcgg accaccatga acaacggcgg cggaacgccg
     1141 ggcgcgcgcc tgaaaccgaa ggacgacgcg gcggtgcgct ccatccgcga ccggcagccg
     1201 gtgagcgtcg gttgatccat gggagccaac ccgaacacgt tcaacggcgg cgcctcgccc
     1261 aatcacgagg tcaacatggt gccgctgatc gacgtctcgt tggtgctggt cgtgatgctg
     1321 ctgctcgcga cgccactggc gttcgaatcg cgcatcgacg tggccaacgc atcacggacc
     1381 gcgaagcagg cggagaaggt cgacaagagc gagcgcgtcg agatcacggt aatctccgaa
     1441 gatagcgtcc gcgtcaaccg tcgtatcgtc gcgcgcgcgc aactcggaga gtcgcttaca
     1501 ccgctcctgg agaccagcgc cgaacgcggc gtgcgggtgg cctgtgcgcc ccgcgtgtcg
     1561 cacggagcgt tcgtggacgt cctcgaccag gccaaaatgt cgggtgccgt cgacatttcc
     1621 gtgatcgaga ggtaaccatg aagcgctacg tcaccgatac ctccaccccc gtcgcgcgca
     1681 tcaacgtcgt gccgatcatc gacgtcgccc tggtgctggt aatcattctg atggtgaccg
     1741 cacccatgat cgcggtgtcc agcctcgacg tcgaactgcc gcgcgcgaag acgcgcagca
     1801 tggagggcgt cgaccgcgtg cacgtgacct tcggtaccac cggcgcattg gccgtcgacg
     1861 agaaggacgt gaccgcgcaa gagttcagcg ccgcgctggc agcttcgatg gccgaacacg
     1921 acgacgacat cctcgtggtc gtgcgcgcgg acgcgcgcgt ctcctacgag gccgtggaag
     1981 cacttgtgaa ggagattcga tcggcgggcg cgaagcgggt cgcgatcggg accacgcagc
     2041 acggagggac gaagagcgat gagtgagctc ttgctcgatc gcgaattttc gaggatcggc
     2101 gggcgcacgc gtcactccat cgcggccagc gcggtactgc acgccctcct gtttctcctg
     2161 ctgggtcttt atcaggccgt cggcggagac accgacgggt tgaccgagat cacgtgggtc
     2221 gatgcgaccg ttcccgacgg cgttcccgac gccgcgccac cgatggccga cgaggaaacg
     2281 aagagcgcgc cggtgcgcga ggtgaagacc gtggccacgc gcgaagcgga gagcgccgag
     2341 cacttcgagc gcgcgttgga acgcggcacc gcggcgcccc gaccgcagtc gtctcgcgcc
     2401 gtgacggata tcttgaacga gcggatcagc gcgctcgaga acaacgcgag cgacagcgcg
     2461 acacgcctgg cgtcattggt aacaccgccc aacgtcggcg tgcccgcgcc ggcgggtgtc
     2521 cccgcctcgg aaaccgtcgg cggtcgcgcc actccgtcga cgctccatcg cgacgcgtca
     2581 cccggcggca gcgtgagcgc gcccagcgcg ctgcggcggg tcgagggcgc accgtcgcgt
     2641 cccgtgatgg tcgcgatcgc gggcgctccg ccggtctccg cggttccggc cgcgaaaccg
     2701 gaggacgtga gccgcacccg cgatctggcc ggtgcccgca tcatcggccc ggtggcggat
     2761 cgcgcggtcg tgagctacca ggtgcccgat taccccgagt gggccaagcg cgaaggtatc
     2821 gaaggatcgg tgacgctgta cttcttcgtg ctcccggacg ggcacgtcaa agaaaacgtc
     2881 ctggtcgagc gcacgagtgg tttcaccgac ttcgacgagg gcgcggtgaa ggcgctgctg
     2941 gcgtggcgat tcgcgacgct gccgggtgga agcgagcaat ggggacgcat caccttccat
     3001 taccggctgg ggaacgcgaa ctaaaagggc ccttcaagga gacgaaccat gagacgttgg
     3061 aaatacagcc tggtgggtac actcgcgtcg ttgtgcgtcg tgtcggcgac gcaggatgcg
     3121 agcccggccg ccgccaagag caagaaggcc gcgcccgaca gcaccatgaa catcaaggcc
     3181 ggcagcggcg gcaaggactt cgagagcatt accgtcgaag gcgaagatcg cgtgcgggtt
     3241 cagttcgagc gtccaccgct caagatcgag ctcgatcccg cctccgctcc cggactggac
     3301 tgggagagcg tatgggacgt gctcggcgcg gaatcgttcg accccgtgga gccggtgctc
     3361 tcgcggcccg cgttcgagcg cacgccgttc gcgccgcggc cgtggctcga ccagtttcgc
     3421 gcgggcccgg tggcgcgctt ccgccccgac gtgagcggcg tggaaaagtg ggcgctcgaa
     3481 gtcgccgatt cgcgcggaaa aaccgtggcg cgctttgccg gcgaggggaa accgccccaa
     3541 gagatcgtgt ggaacggagc gactatcgat ggcacgcccg cgcgcgcgga tctcacgtac
     3601 tcctacgtct tgaacgcatc cgacaaggcc ggcaacaagc ggagcttcct cggcgacagc
     3661 ttccaactcc cgccgcgcgc catggacacg cgggacggga ttgggctgtc gttctcggtc
     3721 cccgccgaaa cggcggccgg cgtcccccga gcactcgttc tcgaagcggc ctcgcgactg
     3781 aaccagaccg gcgactcgag cacgagggtg cacgtcgagg tgacggcgcc gacgttcccg
     3841 ctcgcgaaat cgatcgcgga cgacgtcgtg tcggcgctgc gtccgcactt gctcggtgac
     3901 ccgtcgcgcg tcacggcgac gacgaacgtc gacgcgggcg gcggcgagaa ggcctcggtg
     3961 accattcgca ccgggcccta gccgcgtcag cggatcagct tctcttcctt gaagcgctgg
     4021 tagccttttt cggtgagctt cgcgagctcg gcgaagagtt tcgcgtcgac gcccttgaag
     4081 ttgaagcacg acttcccctg catgcgcctc ttcaactccg gcgaggcgcc ctcgagcagg
     4141 tccgggaacg cgtacacggg catcaggtgg tacgagacgt agttcttgcg cgtctgcacg
     4201 gcgccgaacc acacctcgcg gccgcggctg gtctcggtgg gcgggccgac aagacaaaag
     4261 ccgccggtcg ggtcgggccg gctctggagg ccgcgccggt acttgcgcag gatcgcctcg
     4321 agcttcttgt gcgcggcggt gaactccgaa ttcgacgtgg tcgtcgtctt cgtttttaac
     4381 gtgctcttgg ccttcgtctt cgctttcgct ttcacctaca aacctcccaa gaattgcgtc
     4441 atatccaggg cgtagtgaac cggccatagc catgcaacgt cggcgctgcg ccacagcgtt
     4501 gccaggacga gcaagcccag gccgaagtcg cgtaccaatg caagcaagtc cgccgccgac
     4561 gcgccctgcg ccaccatcgc ggggatgtgc gcccccgcaa acaacagcgc gaccaagatg
     4621 atcgccttgc gttgcccgag tgcagcggcc aaccgaacga agaggatcgc gatcgcgacg
     4681 tcttccagca gcacctgcac ggccaggtgc gcgcgtgacg gatcgaacac accgcgcaag
     4741 gtatccgcgt acgacggcgc gccggcttcg aactgcgagt acaagaacaa cgccaccaac
     4801 gcgaacgcga cgccggtggt cacgcgcaaa agaagcgcgt gccgctggat ccacgcgctt
     4861 gcccacccgt ttcggcggag caccggtacc aacatgatcg gcgcgaagat gacgatctgg
     4921 ttgatcgatt cgcccaccgt gccgagccat ccggagccct cgagacgaat cccgcgcgac
     4981 cacagttgcc cgaggagcag cgtacccacc acacccagga gaaccagcgc gacttcccgc
     5041 catggccgcg caaacgtcgg gcgcgccgga tccttccaca gcgggacgaa gcggttcgtc
     5101 gcccaccaca acccgcaggc gaccgcgtac cccagcgcga ggaacagcga gtgcgacatc
     5161 tcacaccatc cgggtcttcc aggtgccgcg acggaacagc cagatcgcga tcaacccggc
     5221 catcgattcc gtcgccacga tggcccagta tacaccctgc tcgccggctc cccacttcaa
     5281 cgcgaggaga tacgcgaacg ggatctcgaa cagccagaag cagaagaagt tgatcttggt
     5341 gggcgttatg gtgtcgccgg cgccgttgaa ggcctggatc atcaccagct cccacgcata
     5401 gaacaggtag ccgtacgaca cgatgcgcaa gcattgggcg cccgccgcga ccacggccgc
     5461 ttcgtcggtg aagatgcgaa ccagcatctc gttgccgaag atgtagacca ccgataccaa
     5521 gagcatgaac gccatcgtcg caacgccggt gatccacacc gagcgcgccg cgcgatccgg
     5581 ctgcttggcg ccgaggtttt gtcccaccag cgtcgcggcc gcgttggaga gaccccacgc
     5641 cggaagaaac gtgaagatga tgatgcgcac ggcgatggtg taacccgcga gcgcttcggc
     5701 gccgaaggtc gacatgatgc gcacgaggcc gatccagctc gaggtcgcga tgatgaactg
     5761 cccgatgccg cccaaggaca ccttcaccac gcgccacatg acctcggcgt gcacgtgcac
     5821 ttgggacacc agcaccctga tgtgcttgcc gccgcggaac aggaccacga actgcatcag
     5881 cacgcccacg ccacgaccga tgttggtcgc gacggccgcg ccttcgatcc ccatctccgg
     5941 caacggcccc cagccccgaa tcagcgcggg gtcgagcacg atgttgatcg cgttggccac
     6001 ccacaggacg cgcatcgcga tggcggcgtc acccgcgccc ctgaagatgg cattgatcac
     6061 gaagatcagc atgatcacgg cgtttccgcc cagcatccac tgggtgtatt tgtagccctc
     6121 tgcgagggcc cacgcgtcgg cgcccatgag cgcgaggagg tccttggaat agaagatgcc
     6181 gacgagggaa accgggatcg acgcaaccag acccacgatg atggactgaa cggcggtgtc
     6241 ggcggcttcg cgcgggttct tctcgccgat gcggcgcgcg accatcgcgg tggtgcccat
     6301 cgagaggccg atggccaccg cgtagatgag cgtcaatatc gattcggtga gccccaccgt
     6361 cgcgaccgcc gaggccccca gcg
//
LOCUS       DASPGK010000314         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_664415, whole genome shotgun sequence.
ACCESSION   DASPGK010000314 DASPGK010000000
VERSION     DASPGK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664415"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VEC56_08915"
     CDS             complement(<1..911)
                     /locus_tag="VEC56_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14313.1"
                     /translation="MAKRDSRSLVEGPFRIRTLMFIAAVGFGVLIVRLFTLQVVQHEQ
                     YAQFARDNQLQRERLPGPRGFMRDRTGRIVVDNALHFEIVMQWRTRDDAERAVRSLAA
                     YIPVDTVKAMARFDAWQKRYGRTAFPLFPDADKFLISFVRENWSEFPSLRVESQLRRR
                     YPDASVAAHVFGYVGEVTTEEITGAGQDVYVAGDFLGKAGLERQYEDQLRGTPGQRAI
                     EMTASGTALGEISELSTAPTPGHDVMMTLDYDMQEYLDSLLAARPNPAAAVVLDVETG
                     GIIAAASRPAYDPNAFAMGLSASLVGDL"
     gene            complement(892..1392)
                     /gene="mreD"
                     /locus_tag="VEC56_08920"
     CDS             complement(892..1392)
                     /gene="mreD"
                     /locus_tag="VEC56_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXV14314.1"
                     /translation="MKFLIPMLTIVVFLGLQSTVAGKIAIGPVAPDFIVVCVILFGLQ
                     RGPIQGSLFGFLAGFLVDLGNPGYLGLNALTKSLVGYAGGRLGATASPGMLVLVFVIF
                     LSAFGHDIVYFLVYHWPRVDSALVSILVNALPSAIYTAVAGIVVERVLALLGAKVVTR
                     VGKERQ"
     gene            complement(1389..2252)
                     /gene="mreC"
                     /locus_tag="VEC56_08925"
     CDS             complement(1389..2252)
                     /gene="mreC"
                     /locus_tag="VEC56_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXV14315.1"
                     /translation="MQLFESVFQRHRDRTVLVALIAASFALLAMPETRQLDFARALLS
                     RIMRPAQRTSDLIGDYTSVRAENARLRRMLATLALERGRLLQFRDERERLRRLAEFKE
                     EQTFRLVPAEVIGRDFDRIQTNLVIDKGSREGLAERMPVFSYDGLVGSLSKVTENSAW
                     VQLLASKNHPVSCIDKRSRVVGVLEWRHRNNFELKHVSAMEDVAVGDTLLTSGFGGAV
                     PKGFPVAIVVRVAQSTDGLSLRVDARSPIDFMALEEVFVMTDEVPWDRSMFYEEADTA
                     LMRDVLEKARR"
     gene            complement(2256..>2428)
                     /locus_tag="VEC56_08930"
     CDS             complement(2256..>2428)
                     /locus_tag="VEC56_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXV14316.1"
                     /translation="TGGGAMLKGLDQLLKRETGLPINVVDDPLLCVVLGAGKVLDNVH
                     EYDRVLMHSSRD"
BASE COUNT          440 a          804 c          805 g          379 t
ORIGIN      
        1 aggtcgccca ccagcgaggc cgacaacccc atcgcgaatg cgttggggtc gtacgcgggc
       61 cggctggcgg ccgcgatgat gccgcccgtc tccacgtcga ggaccacggc ggcggccggg
      121 ttgggccgtg ccgcgagcag cgagtcgagg tactcctgca tgtcgtaatc gagcgtcatc
      181 atgacgtcgt ggcccggcgt gggtgccgtc gacaattcgg agatctcgcc cagcgcggtg
      241 ccggacgcgg tcatctcgat ggcacgctgc cccggagtac cgcgcagttg gtcttcgtac
      301 tgccgctcga gcccggcctt gcccaggaaa tcgccggcca cgtagacgtc ttgccccgcc
      361 cccgtgattt cttcggtggt cacttcgccc acgtaaccga acacgtgcgc agccaccgag
      421 gcgtcgggat agcgacgccg tagctgcgac tcgacgcgca gggacggaaa ttcggaccag
      481 ttttcgcgca cgaacgagat caagaacttg tcggcgtcgg gaaagagcgg aaatgccgtg
      541 cgaccgtagc gcttctgcca cgcgtcgaag cgcgccatcg ctttgacggt gtcgacggga
      601 atgtacgccg cgagcgaacg gacggcgcgc tctgcgtcgt cgcgcgtacg ccactgcatc
      661 acgatttcga agtggagcgc gttgtcgacg acgatgcggc ccgtgcgatc gcgcatgaac
      721 ccgcgcgggc ccggcaaccg ttcgcgctgc agctggttgt cgcgggcgaa ctgcgcgtac
      781 tgttcgtgct ggacgacctg gagcgtgaag agccgcacga tcaggacgcc gaaaccgacg
      841 gcggcgatga acatcagggt ccggatgcga aacggaccct cgaccaggct cctactgtct
      901 ctctttgcca accctcgtca ccaccttcgc ccccaagagc gcgagcacgc gctcgaccac
      961 gatgccggcc accgcggtat agatcgcgga cggcagcgcg ttgacgagga tgctcacgag
     1021 cgcgctgtcg acgcgaggcc agtgataaac gaggaaatac acgatgtcgt gcccgaaggc
     1081 cgagaggaat atcacgaaga cgagcaccag cataccggga gaggcggtcg cccccaggcg
     1141 cccgcccgcg tacccgacca gcgacttggt gagcgcgttg aggcccaggt atccggggtt
     1201 gccgaggtcg accaggaaac ccgccaggaa gccgaacagc gagccctgga tcggcccgcg
     1261 ctgcaggccg aacaggatca cgcacaccac gatgaagtcc ggtgccacgg ggccgatcgc
     1321 gatcttcccg gcgaccgtcg attgcaggcc caggaacacg acgatcgtga gcatcgggat
     1381 gaggaacttc atcgccgcgc cttttccaac acgtcgcgca ttaaagctgt gtccgcctct
     1441 tcgtagaaca tcgaccggtc ccacggcact tcgtcggtca tcacgaacac ttcctcgagc
     1501 gccatgaagt cgatggggct gcgcgcgtcc acgcgcaagg acaggccgtc ggtggactgc
     1561 gccacgcgca cgacgatcgc gaccgggaac cccttgggga ccgctccccc gaaaccgctg
     1621 gtgagcagcg tgtcgcccac ggcgacgtct tccatggcac tgacgtgctt gagctcgaag
     1681 ttgttgcgat ggcgccactc gagaacgccc acgacgcggc tgcgcttgtc gatgcagctg
     1741 acggggtggt tcttgctcgc caggagctgc acccacgccg aattctccgt caccttggat
     1801 aggctcccga ccagcccgtc gtaagagaag acgggcatgc gctccgcgag cccttcgcgc
     1861 gagcccttgt cgatcacgag gttagtctgg atgcggtcga agtcgcggcc aatgacctcg
     1921 gccggcacca ggcggaaggt ctgctcttcc ttgaactcgg ccagccggcg caggcgctcg
     1981 cgctcgtcgc ggaactggag cagccgcccc cgctccagcg ccaaggtggc gagcatgcgg
     2041 cgcagccgcg cgttctccgc gcgcacggac gtgtagtcgc cgatcaggtc ggaggtgcgc
     2101 tgcgcggggc gcatgatgcg ggagagcagg gcacgcgcga aatcgagctg gcgggtctcg
     2161 ggcatcgcca ggagcgcgaa cgaggcggca atgagggcga cgagtaccgt gcggtcgcgg
     2221 tgtctctgga aaacggactc gaacagctgc atacgctagt cgcgcgagct gtgcatcagc
     2281 acccgatcgt attcgtgcac gttgtcgagc accttgcccg cgccgagtac cacgcagagc
     2341 aaagggtcgt ccacgacgtt gatcgggagg cccgtctccc tcttcaagag ttggtcgagt
     2401 cccttgagca tcgcaccgcc gccggtca
//
LOCUS       DASPGK010000315         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_547251, whole genome shotgun sequence.
ACCESSION   DASPGK010000315 DASPGK010000000
VERSION     DASPGK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547251"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VEC56_08935"
     CDS             <1..409
                     /locus_tag="VEC56_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14317.1"
                     /translation="GEEPRAEKVHESRSSAPPAPSRASGLFDAASGPDTLPQRTSSIF
                     EEKTSEGAPTEALPGLGEILDRLSQGQRDVLQSNAGVPASSDPVATPARPAASTPGSS
                     IGDAPESEWNMDQFRRDLQNIASTLRQSEPRDG"
     gene            464..1285
                     /locus_tag="VEC56_08940"
     CDS             464..1285
                     /locus_tag="VEC56_08940"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXV14318.1"
                     /translation="MHGLYDRIQEAAAFVRTRTQTQPRFGLVLGTGLGDLADAMEVDA
                     VVPYAEIPHFVRSTTEGHKGNLVLGKLGGKPVVSMQGRLHYYEGYSMQDVTFPIRVLR
                     ALGAEDLVVFNAVGGMNTHLDLGDLLVVTDHINLMGDNPLIGWNDDRLGERFPDMSEP
                     YSRARRELAERVALELGTPLRRAVLVAVAGPNLETAAEYRFLSRIGADVVGMSMVPEN
                     LVAVHAGMRVLGVCVVTDLCRPDALEKADIAKIIATANAAEPKLRAFVTEYLKRA"
     gene            1313..>3632
                     /gene="ileS"
                     /locus_tag="VEC56_08945"
     CDS             1313..>3632
                     /gene="ileS"
                     /locus_tag="VEC56_08945"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXV14319.1"
                     /translation="MKPTPFPKLASSQNVPQAEEAIATFWERERIFEKSVEARPAAKP
                     FVFYEGPPTANGRPGVHHVIARLCKDLVCRYRTMRGHRVVRKAGWDTHGLPVEIEVER
                     ELGIVRKEEIEAYGIAEFNRKCRESVFRYEKDWVAFTRRIAFWLDMDHPYVTYHNPYI
                     ESVWWILKEFWKAGLIYQGHKSVPFCPRCQTSLSSHEVSLGYKDAVDPSIFVKFKRAD
                     ADEYFLAWTTTPWTLPSNAALAVAADETYARVSHQGDVLILAEALLKLLDGEYDVLAT
                     MRGADLVGATYEPLFDFFKGTPGAFRVVAGTFVSLEDGTGIVHIAPAFGADDFALHKE
                     QGIPLLEGVKTDGTFEPRIATWAGQFIKDADPLIVKALKQNGRLYKSGKVTHSYPFCW
                     RCSTPLMFYARKTWYIRTTDFKDRMIEANRTINWIPKEVGEHRFGNWLENNVDWSLSR
                     ERYWGTPLNIWTCASCGKQDAVGSIAELKERAVAFPAGELDLHRPFVDEIELKCGCGK
                     SMKRVKEVIDVWFDSGAMPFAQYHHPWDESKTFASQFPADYICEGIDQSRGWFYSLLA
                     ISVFLTGKSPYRNCLTTELILDKHGQKMSKSKGNTVEPWDILNDEGADALRWYLLTTS
                     PPWSPTRFDREGVRDTARKVLENLRNVYAFFAMYASIDGYAHGTDRGAPALLDRWILS
                     RYHSTVARVTECMDEYDVTRAARALERFVLDEVSNWYVRRSRRRFWKGAAGADKTAAY
                     HTLYTVLDGVARLLAPLVPYLAEELYLALRGVT"
BASE COUNT          630 a         1206 c         1207 g          589 t
ORIGIN      
        1 gggcgaggag ccgcgcgcgg agaaggtcca cgaatcgcgc agctcagcgc cgcccgcccc
       61 ctcccgcgcg tcgggattgt tcgacgcggc gtcggggccc gacacgctgc cccagcgcac
      121 ctccagcatc ttcgaggaga agacctccga gggcgcgccc accgaggcgc tgcccggctt
      181 gggtgagatc ctggaccggc tctcgcaggg ccagcgcgac gtgctccagt ccaacgccgg
      241 agttcccgcg tcgagcgatc cggtcgcaac gccggcgcgt ccggccgcgt cgacccccgg
      301 tagctcgatc ggcgacgcgc ccgagtcgga gtggaacatg gatcagttcc gccgcgacct
      361 ccagaacatc gcgtcgacgc tgcgccagag cgaaccgcgc gacggctagc ccgccttttg
      421 ccgttcctgg gccggggcgc ttttgctatc atgctgcccc tctatgcacg gcctctacga
      481 ccgcattcag gaagcggcgg cgttcgttcg cacccgcacc cagacccagc cgcgtttcgg
      541 gctcgtgctc ggcacgggac tgggcgacct cgcggacgcg atggaggtcg acgcggtcgt
      601 accgtacgcc gaaatcccgc acttcgtgcg ctcgaccacc gaggggcata aaggcaatct
      661 cgtcctgggg aagttgggcg gcaagccggt ggtgtcgatg cagggtcgcc tccattatta
      721 cgagggctac tcgatgcagg acgtcacctt cccgatccgc gtcctgcgcg ccttgggcgc
      781 cgaggatctg gtggtgttca acgcggtcgg cggtatgaac acgcacctcg atctgggcga
      841 cctcctcgtc gtaaccgacc acatcaattt gatgggggac aacccgctca tcggctggaa
      901 cgacgatcgc ttgggcgagc gctttcccga catgtcggag ccgtacagcc gcgcgcgacg
      961 cgagctcgcg gagcgcgtgg cgctcgagtt gggaacaccg ctgcggcgcg ccgtgctggt
     1021 cgcggtcgcg ggtccgaacc tcgagaccgc ggccgagtac cgcttcctgt cgcgcatcgg
     1081 cgccgacgtg gtcggcatgt cgatggtgcc cgagaacctg gtcgccgtgc acgcgggcat
     1141 gcgggtgctg ggcgtgtgcg tggtgacgga cctctgtcgc cccgacgcgc tcgagaaggc
     1201 cgacatcgcc aagatcattg ccaccgcgaa cgcagccgaa ccgaagctgc gcgcgttcgt
     1261 gaccgagtac ttgaaacgcg cctgagagcc gcgccgcgtg gcgcgtgaca cgatgaaacc
     1321 gactccattc cccaagctcg catcgagcca gaacgttccg caagccgagg aggcgatcgc
     1381 gaccttctgg gagcgcgagc gcatcttcga gaagagcgtc gaggcgcgcc cggccgccaa
     1441 gcccttcgtg ttctacgagg gcccgcccac ggcgaacggc cgccccggcg tgcaccacgt
     1501 gatcgcgcgt ttgtgcaaag acctcgtgtg ccggtatcgc accatgcgcg gtcaccgcgt
     1561 ggtgcgcaaa gcggggtggg acacgcacgg cctgccggtc gagatcgagg tcgaacgcga
     1621 gctcgggatc gtgcgcaaag aagagatcga ggcctacggg atcgcggagt tcaaccggaa
     1681 gtgccgcgag agcgtgttcc gttacgagaa ggactgggtc gcgttcacgc gccgcatcgc
     1741 cttctggctc gacatggatc acccctacgt cacctaccac aacccgtaca tcgagtcggt
     1801 gtggtggatc ctgaaggaat tctggaaagc ggggttgatc tatcaaggac acaagagcgt
     1861 gccgttctgc ccgcgctgcc agacctcgct ctcgagccac gaagtgagcc tcggctataa
     1921 ggacgcggtc gaccccagca tcttcgtgaa gttcaagcgc gcggacgccg acgaatattt
     1981 tctcgcgtgg acgacgacgc cgtggacgct acccagcaac gccgcgctgg ccgtggccgc
     2041 ggacgagacc tacgcgcgcg tttctcacca aggcgacgtg ttgatcctgg ccgaggcgct
     2101 cctgaagttg ctcgacgggg agtacgacgt gctcgcgacc atgcgcggcg ccgacttggt
     2161 gggtgccacc tacgagccct tgttcgattt cttcaaggga acgccgggcg cgtttcgggt
     2221 ggtcgcgggg accttcgtga gtctcgagga cggcaccggc atcgtgcaca tcgctcccgc
     2281 cttcggcgcc gacgatttcg cgctccacaa agagcagggc atcccgctgc tggaaggggt
     2341 caagaccgac ggcaccttcg agccgcgcat cgcgacctgg gccgggcagt tcatcaagga
     2401 cgcggacccc ctgatcgtga aggctctcaa gcagaacgga cggctgtata aaagcgggaa
     2461 ggtgacgcac tcgtacccgt tctgctggcg ttgctcgacg cccctgatgt tctacgcgcg
     2521 caagacctgg tacatccgca ccaccgactt caaggaccgg atgatcgaag ccaatcgcac
     2581 gatcaactgg atccccaagg aagtgggcga gcaccgcttc ggcaactggc tcgagaacaa
     2641 cgtcgattgg tcgctctcgc gcgagcgcta ctggggcaca ccgctcaaca tctggacgtg
     2701 cgcgtcgtgc gggaagcagg acgcggtggg gagcatcgcc gagctgaagg agcgcgcggt
     2761 agccttcccg gccggagagc tcgacctcca tcgtcccttc gtcgacgaga tcgagttgaa
     2821 gtgcgggtgc gggaagtcga tgaagcgcgt caaggaagtg atcgacgttt ggttcgactc
     2881 gggtgccatg cccttcgccc agtaccacca cccgtgggac gaatccaaga ccttcgcgag
     2941 ccagttcccg gccgactaca tctgcgaggg aatcgatcag tcgcgcgggt ggttctactc
     3001 gctgctcgcg atctccgtgt tcctgaccgg caagagcccg taccgcaatt gcctgaccac
     3061 cgaactcatt ctcgacaagc acgggcagaa gatgtcgaag agcaagggca acacggtcga
     3121 gccctgggac atcctgaacg acgagggcgc ggacgcgctg cgctggtact tgctgaccac
     3181 gagcccgccc tggagcccga cgcgcttcga ccgcgagggc gtgagggaca ccgcgcgcaa
     3241 ggtgctcgag aacctgcgca acgtgtatgc gttcttcgcc atgtacgcga gcatcgacgg
     3301 ctacgcgcac ggcaccgacc ggggcgcgcc cgcgctgctc gatcgctgga tcctgtcgcg
     3361 ctaccattcg acggtggcgc gcgtgaccga gtgcatggac gagtacgacg tcacgcgcgc
     3421 ggcgcgggca ctcgagcgtt tcgtcttgga cgaggtctcg aactggtacg tacgccgttc
     3481 gcggcgccgc ttctggaagg gagccgcggg cgcggacaag acggccgcgt atcacacgct
     3541 ctacaccgtg ctcgacgggg tcgcgcgcct gctcgcaccg ctggtcccgt atctcgccga
     3601 ggagctctac ctcgcgctgc gcggtgtcac cg
//
LOCUS       DASPGK010000316         8111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_314474, whole genome shotgun sequence.
ACCESSION   DASPGK010000316 DASPGK010000000
VERSION     DASPGK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314474"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1842)
                     /gene="rpoD"
                     /locus_tag="VEC56_08950"
     CDS             complement(40..1842)
                     /gene="rpoD"
                     /locus_tag="VEC56_08950"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXV14320.1"
                     /translation="MGAKVKNTGSAASAPATAPDRFEFILEDIRKIAEEKGFVLNHEI
                     DHLVGEDFSPEDIVILYDRLSEEKIDFFDSPEKARLKIEARKRREEKEEEKKVEEDVK
                     AVIRYDDPVRMYLREMGKVPLLDREGEVEIAKRIEQGQIMIAKAVFSLDSPIRELQRL
                     ARAVEKGDMRLDEVVQVETGGLAPSYTGKKERQARFRPVKKIATLRKEIVELQKKLKL
                     KKSAAKRPALERSLEMRQAKLFEEYIKLQLNPKQLEKIVEKIRGQRDRLKDYEAKLLE
                     YEDFVGLGYDDIVTQSKKLKGSRRKSVKVEGKGTWSLDMLEDFERKMRSLRTEIKAIE
                     KEENISIADLDKIAENIRRGEIQVQRAKKEMIEANVRLVIAIAKRYTNRGLEFLDLIQ
                     EGNSGLMRAVDKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRVPVHMIEAINKVS
                     RAQRKLVQEQGRDPTPEEVAKKLNYPLDKVKSVMKASMEPISLDRPIGEDEDSNLSDF
                     IEDTSAASPAQSASHAMLRDQLNRVLSTLTRREEKVIRLRFGLGDGTPRTLEEVGTIF
                     NVTRERVRQIEAKALKKLQHPSRARKLKGYTEII"
     gene            complement(1848..3599)
                     /gene="dnaG"
                     /locus_tag="VEC56_08955"
     CDS             complement(1848..3599)
                     /gene="dnaG"
                     /locus_tag="VEC56_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXV14321.1"
                     /translation="MPIPQHIIDQVRDRSDVVEVVGQYVDLKRTGTNYKGLCPFHKER
                     TPSFAVSPERQIYHCFGCGKGGNVFNFLMEMDGVSFPEAVRELGRRAGIDVETRPEDD
                     ARSENEALFHANAFAGRFYHDALAKSAGGDRARRYLEGRGIPREVWRRFGLGWAPGTG
                     EALAEAALAHGIARDAVLKLKLVMPREGGRGYYDYFRDRVMFPIIQPGGRVVGFGGRA
                     LGDAEPKYLNSAESPLFQKRRTFYGVDRARDPIRAARFAYIVEGYTDLIRLHLCGVEN
                     SLATCGTALTREHAARIRRLTRRVVLVPDGDPAGENAALISGALLMAEGVEVGVVSLP
                     PGSDPDSAGKSLAQKEFKELLGRSLEYFAYLDYTIQHRNPTAREREELIRRIVGAISS
                     ADDPLRADVLVGELARVVGVEPGGLRRLLRPGGTADGDRRAGSDAGTSRSGARRAALE
                     RLVLRLVMEGTPSALDALDSLDVEDFSDEANRKLYKLLDSMREAHIDLRGRDFQRRAE
                     ETGLAGFANEISLVSVPPGNIDTLLKDHLRELKKRRIEDELEQLRERLLNVPAESEES
                     IAIAEYFKRLRQALEEL"
     gene            complement(3603..5963)
                     /locus_tag="VEC56_08960"
     CDS             complement(3603..5963)
                     /locus_tag="VEC56_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXV14322.1"
                     /translation="MAHPIPTNPFEHAARVLSLDAVLELLAAHCVNEGTRRNARASTP
                     TADVVAIQRSLDEIAEYATLREESGDIAIPDTSYRADVEAIANGARGSGEALRRVADG
                     ERAIAALRRGVGANAETFPRLSEIAGGASPHHDLVAEADRALEPDGSIKDDATPELKT
                     HRRAIRGLRTTLRERVEKLVAEIGADAHATVSGSRHVLVVPRAKVKRGSGFVHGASHS
                     GGSLYVEPIALLELNNDLETRLADEAEEIERILEALSARVRAAAPAILVNADVVERVD
                     LVRAKEAMSERFGCIAPEISSTGRLRLVRARHPLLSLALERAGRFDQQVPLDLVLEHA
                     ERLMIITGPNAGGKTVALKTVGLVSLMLQCGLRVPCAHGTEVPVFERVFADIGDEQSL
                     ESSLSTFTSHLRYLDEMARHADARSLCLVDEIGDGTDPDEGAAIAIAALERMLASGAA
                     VIATTHYGRIKTFALETRGVANASMAFADETSRPLYRLMQGIAGRSRGIDTARRTGFD
                     PTVVARAEAILGGEAFRLESALARLESSYQKLQEERSTLEKARLRLEEATREYEEKER
                     AYALTKKEASKRAMREAEAMLTDARREIEGLVRDIRTRGADKDAIRQSRKRVDDMLAD
                     VRGQAQALEPKSTPLARVAPGDRVSLSPTGRPFGTVVEVDRKDVTVDMGGKKIRARIG
                     KLYPAAGDEAAVVRASVDVQFEPVGEIEVSVRGMDRESALEEVSRFIDRAVLTGLSEV
                     KIIHGLGTGVLAKAVRDALARDPRVVSHRTGGPMEGGTGVTFARLK"
     gene            complement(5976..6515)
                     /locus_tag="VEC56_08965"
     CDS             complement(5976..6515)
                     /locus_tag="VEC56_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXV14323.1"
                     /translation="MDDTTIINIVLVGIVAIGAIAGGIKGFARQVIELVGLVVSFFVA
                     AVVASWLAALLSDHMSIPHAPALVMAFLAVFVGGLIAFHFVALSAQRLIHTTLFGWVD
                     HACGAMFGLLAATLVASVMTSAALELPVSDRVRGGVEDSAVAAFVQPIAGWLFHAVFP
                     RDPGRLAAESMARALGPDA"
     gene            complement(6557..7000)
                     /locus_tag="VEC56_08970"
     CDS             complement(6557..7000)
                     /locus_tag="VEC56_08970"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXV14324.1"
                     /translation="MMIWEKLNEDMKTALKGGDKVRLSVVRMLISELKNERIAQGADL
                     DDVAQRKVLASYAKRRKEAIEAARAGGREDVATKEQAEYDITMSYLPKQLSDDELRAA
                     IRKHIESSGASGPGGFGVVMKSVMAELGGQADGKLVSALVRELLG"
     gene            complement(7046..7774)
                     /locus_tag="VEC56_08975"
     CDS             complement(7046..7774)
                     /locus_tag="VEC56_08975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXV14325.1"
                     /translation="MPVHRFTFYLPSLSRDATAFDLTGDEHHHLARVLRLSAGETISV
                     TDGRGLLVEAAIATTSKNRSTARVIRVLDDRAPARRVVLAMSLLPRAHFEVAVSQCVE
                     VGVTDLIPVVAEKCHATKWSKAAAARVERVAIAAMKQSGRAWLPPVHEVRTLGALASE
                     VASGGYGRAVVGDAGAPALTAVSRDRDVLAIVGPEAGLTDAEVARLVHAGAERASVSA
                     HRLRAETAAVVLVAALARGADGGV"
     gene            complement(7785..>8111)
                     /locus_tag="VEC56_08980"
     CDS             complement(7785..>8111)
                     /locus_tag="VEC56_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXV14326.1"
                     /translation="EEEADDRFTRHGNDVLIDIPLTYPQLALGAKLEVPTLDGKVLLR
                     IPAGTPSHKVFRIKGKGIPRLNGYGRGDQLVRAVAWVPDKVSKKEEELLKELEKSLSN
                     RAPKID"
BASE COUNT         1241 a         2827 c         2606 g         1437 t
ORIGIN      
        1 cacgagagcc agcgcccgct tgggacatcc gattcgaagc tagatgatct cggtgtagcc
       61 cttgagcttc cgcgcccggc tcgggtgctg gagcttcttc aaggccttcg cttcgatctg
      121 gcgcacgcgc tcgcgcgtca cgttgaaaat ggtcccgacc tcttcgagcg tgcgcggggt
      181 accgtcgccc aacccgaagc ggagccggat cactttctct tcgcggcgcg tgagggtgct
      241 caacacgcgg ttcaactggt cgcgcagcat cgcgtgcgac gcggactgcg ccggcgacgc
      301 ggccgaggtg tcctcgatga agtcggacag gttggagtcc tcgtcctcgc cgatcgggcg
      361 atcgagcgag atgggctcca tgctcgcctt catcacactc ttgaccttgt cgagcgggta
      421 gttgagcttc ttcgcgacct cttccggggt cgggtcgcgg ccctgctcct gcaccagctt
      481 gcgctgcgcg cgcgagacct tgttgatggc ctcgatcatg tggaccggga cgcgaatggt
      541 gcgcgcctgg tccgcgatcg cgcgcgtgat ggcctggcga atccaccagg tggcgtaggt
      601 cgagaacttg tagcccttgc gatagtcgaa cttgtcgacc gcgcgcatga ggccgctgtt
      661 cccttcctga atgaggtcca agaactcgag gccgcggttg gtgtagcgct tcgcgatggc
      721 gatgacgagg cgcacgttgg cctcgatcat ctccttcttg gcgcgctgga cctggatctc
      781 gccgcggcgg atgttctccg cgatcttgtc caagtccgcg atcgagatgt tctcttcctt
      841 ctcgatggcc ttgatctcgg tgcgcagcga gcgcatcttg cgctcgaaat cctcgagcat
      901 gtcgagcgac caggtcccct tgccctcgac cttcacactc ttgcggcgcg agcccttgag
      961 cttcttcgac tgggtgacga tgtcgtcgta gcccaaaccc acgaagtcct cgtactcgag
     1021 gagcttggcc tcgtagtcct tcaggcggtc gcgctggccg cggatcttct ccacgatctt
     1081 ctcgagctgc ttgggattga gctggagctt gatgtactcc tcgaacagct tggcctggcg
     1141 catctcgagc gaacgctcga gcgcgggccg cttggccgcg ctcttcttga gcttgagctt
     1201 cttctggagc tcgacgatct ccttgcgcaa ggtcgcgatc ttcttgacgg ggcggaagcg
     1261 cgcctggcgc tccttcttgc cggtgtacga cggagcgaga ccgcccgtct ccacctgcac
     1321 cacttcgtcc aggcgcatgt cgcccttctc caccgcgcgc gccaagcgct gcagctcgcg
     1381 gatgggcgaa tcgagcgaga acaccgcctt cgcgatcatg atctgaccct gctcgatacg
     1441 cttggcgatt tcgacttcgc cctcgcgatc gaggagcggg accttgccca tctcgcgcaa
     1501 gtacatgcgc acggggtcgt cgtagcgaat gacggccttg acgtcctctt cgaccttctt
     1561 ctcttcttcc ttctcttcgc gccgtttgcg cgcttcgatc ttcaagcgcg ccttctcggg
     1621 cgagtcgaag aaatcgattt tctcttcgct caagcggtcg tagagaatga cgatgtcttc
     1681 ggggctgaag tcctcgccca cgagatgatc gatctcgtgg ttcaggacaa agcccttctc
     1741 ctccgcgatc ttgcggatgt cttcgagaat gaactcgaag cgatccggcg cggtcgcagg
     1801 agcgctcgca gcagaacccg tattcttgac tttcgcaccc acgcgcctca caactcctcc
     1861 agcgcctgcc tcaggcgttt aaagtactcg gctatcgcta tggactcttc gctctctgcc
     1921 gggacattga gaagccgttc acgaagctgt tccaactcgt cctcgatccg gcgttttttc
     1981 aactcccgca agtggtcttt gagaagggtg tcgatgttcc ccggtgggac ggagacgagg
     2041 gatatttcgt tggcgaaccc cgccagcccg gtctcttctg cccgacgttg aaagtcgcga
     2101 cccctcaaat caatatgggc ctcccgcatc gagtcaagca gtttatatag cttgcggtta
     2161 gcttcgtccg agaaatcttc gacatcgagg gaatcgagcg cgtcgagcgc cgacggggtg
     2221 ccctccatca ccagccggag aacgaggcgc tcgagtgccg ctcgtctagc tccggaccgg
     2281 cttgttcccg catcagaccc cgcacgccga tccccgtcgg ccgtaccacc cgggcgaagc
     2341 aacctgcgca gccccccggg ttcgaccccg accacccggg ccagctcgcc gaccagaaca
     2401 tccgcccgaa gggggtcgtc agccgacgaa atggcaccga cgatgcgcct gatgagctcc
     2461 tcacgctcgc gcgcggtcgg gtttcgatgc tggatagtat agtcgaggta tgcgaaatat
     2521 tccaacgaac gccccaaaag ttctttgaac tctttctgcg cgagtgactt acctgcggaa
     2581 tcggggtcgg atccgggggg tagcgaaacc accccgactt ctaccccctc ggccatcaga
     2641 agtgcgcccg aaatgagggc cgcgttctcc ccggccgggt cgccgtccgg tacgaggacc
     2701 acccgccgtg tcaggcggcg gatccgggcc gcgtgctccc gggtcagggc ggttccacag
     2761 gtcgcgagcg agttctccac cccgcacagg tggagccgaa tcaggtccgt gtacccctcg
     2821 acgatgtagg cgaagcgggc cgcccggatc ggatcgcgcg ctcggtcgac cccgtagaag
     2881 gtgcgccgct tctggaacag cggactttcc gccgaattga ggtacttggg ctccgcgtcc
     2941 cccaacgcgc gaccgccgaa gccaaccacc cgcccgccgg gctggatgat cgggaacatg
     3001 acccggtcgc gaaagtagtc gtagtagcct cggccgccct cgcgcggcat gaccagcttg
     3061 agcttcaaga cggcgtcgcg ggcaatcccg tgggcgaggg ccgcctccgc gagcgcttcg
     3121 ccggtccccg gcgcccagcc cagcccgaat cggcgccaca cctcgcgcgg aatcccgcgt
     3181 ccctccaaat agcgccgcgc gcggtccccg cccgccgact tcgcgagcgc gtcgtggtag
     3241 aagcggccgg cgaaggcgtt ggcgtggaag agcgcctcgt tctcggagcg tgcgtcgtct
     3301 tcggggcgcg tctctacgtc gatgcccgcg cggcggccca gctcgcgcac ggcttcgggg
     3361 aagctcaccc cgtccatctc cattagaaaa ttgaacacgt tgccgccctt gccgcagccg
     3421 aagcagtggt agatctgccg ttcggggctc accgcaaagc tcggcgtgcg ctccttgtgg
     3481 aacggacaca gacccttgta attggttccg gttcgtttca aatccacgta ctgccccacc
     3541 acctcgacga cgtcggagcg atcgcggacc tgatcgatga tgtgctgcgg gataggcata
     3601 ggctacttga ggcgcgcgaa ggtgacgccg gtgccgcctt ccatcgggcc gccggtccgg
     3661 tgcgagacga cgcgcgggtc gcgcgcgagc gcgtcgcgca ccgccttcgc caagacaccg
     3721 gtccccaagc cgtggatgat cttcacttcc gagaggccgg tcagcaccgc gcggtcgatg
     3781 aagcggctca cttcctcgag ggcgctctcg cggtccatgc cgcgcacgct gacctcgatt
     3841 tcacccaccg gctcgaactg gacgtcgacg ctcgcgcgca caacggcggc ctcgtcgccg
     3901 gcggcaggat acagcttccc gatgcgcgcg cggatttttt tcccgcccat gtccacggtg
     3961 acgtccttgc gatcgacctc caccacggtg ccgaagggcc ggccggtggg agagagcgac
     4021 acccgatcgc cgggtgccac gcgcgcgagc ggtgtggact tgggctccag ggcctgcgcc
     4081 tgcccgcgca cgtcggccag catgtcgtcg acgcgcttgc ggctctggcg gatcgcgtcc
     4141 ttgtcggcgc cgcgcgtgcg aatgtcgcgc accagcccct cgatctcgcg ccgcgcgtcg
     4201 gtgagcatcg cttccgcttc gcgcatcgcg cgcttgctcg cctctttctt ggtgagcgcg
     4261 tacgcgcgct ccttctcttc gtactcgcgc gtcgcctctt cgaggcgaag gcgcgctttt
     4321 tcgagcgtgc ttcgctcctc ttgtagcttt tggtagctgg actccaagcg cgcgagcgcc
     4381 gactccagac gaaacgcctc gccccccaag atcgcttccg cgcgcgcgac caccgtgggg
     4441 tcgaagccgg tgcggcgcgc ggtgtcgatg ccccggcttc gccccgcgat cccttgcatc
     4501 agtcgataca gcgggcgcga ggtctcgtcg gcgaaggcca tcgacgcgtt ggcgaccccg
     4561 cgcgtctcca gcgcgaaggt cttgatgcgc ccgtagtgcg tggtcgcgat cacggcggcc
     4621 cccgacgcca gcatgcgctc gagcgccgcg atggcgatgg ccgcgccctc gtcggggtcg
     4681 gtgccgtcgc cgatttcgtc gacgaggcat aacgagcgcg cgtcggcatg gcgcgccatc
     4741 tcgtccaggt agcgcaagtg cgacgtgaac gtcgacagcg acgactccag ggattgctcg
     4801 tcgccgatgt ccgcgaacac gcgctcgaag accggcactt cggtgccgtg cgcgcacggc
     4861 acgcgcagcc cgcattggag catgagcgag acgagtccga cggtcttcag ggccaccgtc
     4921 ttaccgcccg cgttggggcc ggtgatgatc atgaggcgct cggcgtgctc cagcacgagg
     4981 tcgagcggga cctgctggtc gaagcgcccc gcgcgctcga gcgcgagcga gagcaggggg
     5041 tggcgcgcgc gcaccaggcg cagcctcccc gtcgacgaga tctcgggcgc gatgcacccg
     5101 aagcgctcac tcatggcctc cttggcgcga accagatcga cgcgctcgac cacgtcggcg
     5161 ttgaccagga ttgcgggtgc ggccgcgcgc acacgcgccg agagcgcttc gaggatgcgc
     5221 tcgatctcct ccgcttcgtc cgccaggcgc gtttccaggt cgttgttgag ctcgaggagc
     5281 gcgatgggct cgacgtacaa cgacccgccc gagtgggagg cgccgtgcac aaagccgctc
     5341 ccgcgcttga ccttcgcgcg cgggacgacc agcacgtggc ggctcccgct gacggtggcg
     5401 tgcgcgtcgg cgccgatctc cgccaccagt ttctcgacgc gctcgcgcag cgtcgtgcgc
     5461 aacccgcgaa tcgcgcggcg atgcgtcttc agttcgggcg tcgcgtcgtc cttgatgctg
     5521 ccgtcgggct cgagcgcgcg atcggcctcc gccaccaagt cgtggtgggg cgaagcgccg
     5581 cccgcgatct cggacaggcg cgggaacgtc tccgcgttcg cgcccacgcc gcgccgcagc
     5641 gccgcgatgg cccgctcgcc gtccgcgacg cggcgcaggg cctcgcccga cccgcgcgcg
     5701 ccgttcgcga tcgcttccac gtcggcgcgg tacgacgtgt cgggaattgc gatgtcgccg
     5761 gattcttcgc ggagcgtcgc gtactcggcg atttcgtcca gcgagcgctg gatggcgacg
     5821 acgtcggcgg tcggggtcga cgcgcgcgcg ttgcgacgcg tgccttcatt cacacaatgc
     5881 gcggccaaca actcgagcac ggcgtcgagc gaaagcacgc gcgcggcgtg ctcgaaggga
     5941 ttcgtcggga tagggtgggc catgggccgc agacgctacg cgtcgggccc gagggcgcgc
     6001 gccatgcttt cggcggccag acgccccggg tcgcgcggga atacggcgtg gaacaaccag
     6061 cccgcgatgg gctgcacgaa agcggcgacc gccgagtctt ccacgccgcc gcgcacgcgg
     6121 tcggacaccg gcagttcgag cgccgctgac gtcatgacgc tggcgaccag tgtcgccgcc
     6181 aagagcccga acatcgcgcc gcacgcgtgg tcgacccatc cgaacaacgt ggtgtgaatc
     6241 aagcgctggg cggagagtgc cacgaagtgg aatgcgatca ggccgcccac gaacacggcc
     6301 aggaatgcca tcaccagcgc aggcgcgtgc gggatcgaca tgtggtcgga caagagcgcg
     6361 gcgagccagc tcgccaccac cgccgccacg aagaacgaca ccacgagccc caccagctcg
     6421 atcacctggc gcgcgaagcc cttgatgccc cccgcgatcg cgccgatggc gacgataccg
     6481 acgagcacaa tattgataat ggtggtgtcg tccatgtgct gcggcccgga tcggcaccgg
     6541 gagaaaggtg tgcaggtcag ccgagcagct cgcggaccag agccgagact agcttgccgt
     6601 cggcctggcc gccgagctcc gccatcaccg acttcatcac gacgccgaaa ccgcccggtc
     6661 ccgacgcgcc gctgctttcg atgtgctttc ggatcgccgc gcgcaactcg tcgtcggaaa
     6721 gctgcttggg cagatacgac atggtgatgt cgtactccgc ctgctccttg gtggcgacgt
     6781 cctcgcggcc gcctgcgcgc gcggcctcga tggcttcctt acgacgcttg gcgtagctgg
     6841 ccaagacttt gcgctgggcg acatcgtcga ggtcggcgcc ctgcgcgatg cgttcgttct
     6901 tgagctccga gatcagcatg cgcacgacgc tcaaacggac cttgtcgccg cctttgagcg
     6961 ccgttttcat gtcttcgttg agtttttccc aaatcatcat gttatgcatc ctccccgggc
     7021 agtctatgcc gcgcccccat ccctgtcaaa cgcccccgtc agcgccgcgc gcgagcgcgg
     7081 cgacgagcac caccgcggcc gtctccgcgc gcagccggtg cgccgaaacc gacgctcgct
     7141 ccgcccccgc gtgcaccaga cgcgccacct cggcgtcggt caggccggct tcgggaccca
     7201 cgatcgccag cacgtcgcga tcccgcgaga cggccgtgag cgcgggcgct cccgcgtcgc
     7261 ctaccaccgc gcgaccataa ccgcccgacg ccacctcgct cgcgagcgcg cccagcgtgc
     7321 gtacctcgtg cacgggcggc agccacgcgc gccccgattg cttcatggcc gcgatcgcca
     7381 cccgctcgac gcgggcggcg gccgccttcg accacttggt cgcgtggcac ttctccgcca
     7441 cgacgggaat gaggtcggtc acacccactt cgacgcactg cgacaccgca acttcgaaat
     7501 gcgcgcgcgg caacagcgac atcgccagca cgacgcgccg cgccggcgcc cggtcgtcga
     7561 gcacgcgtat gacgcgcgcg gtacttcggt tcttcgaggt cgtggcgatc gccgcttcga
     7621 ccaggagccc gcgaccatcg gtcacggaga tggtctctcc cgccgacagg cgcagcacgc
     7681 gcgccaggtg gtggtgctcg tcgcccgtga ggtcgaacgc cgtcgcgtcg cgcgacaacg
     7741 acgggagata gaacgtgaac cgatgaaccg gcatcggcgg cgcgctagtc gatcttgggc
     7801 gcgcggttgg agagcgactt ctcgagctcc ttcaggagct cctcttcctt cttgctcact
     7861 ttgtccggca cccacgcgac cgcgcgcacc agctggtcgc cgcggccgta gccgttcagg
     7921 cgcgggatgc ccttgccctt gatgcggaaa accttgtgcg acggcgtgcc cgcgggaatg
     7981 cgcaggagca ccttgccgtc cagggtggga acttcgagct tcgcccccag cgcgagctgg
     8041 gggtaggtca gcggaatgtc gatcagcacg tcgttgccgt ggcgggtgaa gcgatcgtcg
     8101 gcttcttcct c
//
LOCUS       DASPGK010000317         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1365582, whole genome shotgun sequence.
ACCESSION   DASPGK010000317 DASPGK010000000
VERSION     DASPGK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365582"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1019)
                     /locus_tag="VEC56_08985"
     CDS             complement(123..1019)
                     /locus_tag="VEC56_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXV14327.1"
                     /translation="MGVFDRVVSMTLPLVPKPVVRRVASPYIAGEKMDDLVRIAEGLN
                     RDGYRVAAAILGEFVTRREESEQAVHEYQALLASMGERRIDGYIHVKPTHLGLKLDVA
                     FCLENVRTLLVAARAQGLFVRLDMEDSPCIDDTLEIYDRLRPDFDNVGVVIQSRMRRS
                     LADVRALAKVKANVRVCKGIYIEPFALAYTDPELISRNYALLVEELLGAGCTVAIATH
                     DERLVWEAARTIDRLQIPRERYEFQMLLGVLAPLRATIKAAGHPVRVAVPYGPNWYPY
                     SLRRLRKNPAIAGYVLKSLLKR"
     gene            complement(1147..1986)
                     /locus_tag="VEC56_08990"
     CDS             complement(1147..1986)
                     /locus_tag="VEC56_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV14328.1"
                     /translation="MMRCEMVLIAVCVLACAGCGGLSAPNAELFPAPPRDAVTFWGHA
                     CVYIDAGGVGVVTDPVFERSVIFRQRRIPSPPVSSYAGARVVLISHAHPDHLSAETLR
                     TFPESVVILAPRPCAKYLDDVGREVRAVAPGDVFETDGVRVTAVAAHHMGPRLGLDAA
                     SDGRALGYVIETAAATIFYSGDTDYFSGFGDVGWTFDPDIAILNINGHLPSTDATRAA
                     WATRAPVVIPAHWGGFGYWVFGGNKRPRDGETLARVIGDRLRVLEVGQSLPLPTPTFG
                     ASE"
     gene            complement(1983..3089)
                     /locus_tag="VEC56_08995"
     CDS             complement(1983..3089)
                     /locus_tag="VEC56_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14329.1"
                     /translation="MKGFVAVLRKEAIHMRRDKGTLRLALGIPVFQLILFGSIDTNVT
                     LVPTAVFDQCRCPESRALLDELEATKTFRIERPVTSRSALREAIVAGDAKVGIEIPPD
                     YAKRRLEGTQATFLALIDGSDNTIASQSLAAIDGWALTRSLEDLQARTGAGELPVAPR
                     PQILFNPDTRSANLLIPGLIAILLTFSGTLLTAYAIVRERERGTLEQLMVTPVSSLEM
                     ILGKLVPYLVLAFVQLVLVLFCMHVFFKVPIHGSVLLLAGLSLVYLTSLLALGLMISA
                     RSATQIEATQAAQGILLPSIMLSGYIFPLSSLPVPLRVVAEALPATHFVAISRGIIIR
                     GAGFDDLVREVAALAVISAVLLTASVVIFRRKRL"
     gene            complement(3086..4084)
                     /locus_tag="VEC56_09000"
     CDS             complement(3086..4084)
                     /locus_tag="VEC56_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14330.1"
                     /translation="MSPAEERADDRFGIEVRGVSHDYGPVHALRDVSLSIARGTIFGL
                     LGPNGSGKSTLIRILCGLLRPTSGHAFVDGIDVTREADAVRKRIGYVPQRFSLYEDLT
                     VLENLNFFARVYGLSGERLVERREWVIGLTGIAPYRDRLAGQLSGGWKQRLSLGAALM
                     HEPRIVFLDEPTAGIDPVARRELWNLLFDLSAGGVTLLVSTHYMDEAERCSRVGYLYL
                     SRLLVEGEPEDLVRLPEITPAGTRRVEAAIDGNPAPVMRRARSLAYVREVTIFGNSLH
                     LLVPTTITDEAIQRDLSSDVPVDVAIRPIMPSLEDVFVRLTALQDDAVTAAKETAR"
     gene            complement(4081..>4999)
                     /locus_tag="VEC56_09005"
     CDS             complement(4081..>4999)
                     /locus_tag="VEC56_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXV14331.1"
                     /translation="MRRLILPVVLVAGIFVWLVLRGGRGEDHAYSGTIEARDARVGSL
                     VGGRVLDVLVEEGDSVQAGQVLVRLDGDLLVAQVREQEARVAQAQAALDRVVSGPRRE
                     QIERARIEWDRAEKERKRQESLLAQALTSDEAYDAAAAAAQSAFQVYEELRAGSRSED
                     ERQARAAWDEARSHLVYLQRQLAETEVRSPSAGVVQTIDLRPGDMVAANQGIVAILAP
                     DELWVRVFVPETRLGRVRVGQSVDVTIDTFPERAFPARVASVSARAEYTPRNVQTVEQ
                     REDQVFAVKLDIEPAPELKAGMTATVRLE"
BASE COUNT          837 a         1706 c         1705 g          751 t
ORIGIN      
        1 ggcttccacg cgcaggcgag ccatcgactg cgcgttcaag aggtcgttca ggcgcgagag
       61 gcgttcctgt tcgtcggggt cggtgccgtg gatgtaggcg tcgccgtcgc ccatgtcggt
      121 cgctatcgct tgaggaggga cttgaggacg tagccggcga tcgcgggatt cttgcgcagc
      181 cgcctcagcg agtacggata ccagttgggt ccgtacggca ccgcgacgcg caccgggtgc
      241 cccgccgcct tgatggtggc gcgcagcggc gccagcacgc ccagcagcat ctggaactcg
      301 tagcgctcgc ggggaatctg caaacgatcg atggtgcgcg cggcctccca caccaggcgc
      361 tcgtcgtggg tcgcgatcgc gaccgtgcac ccggcgccca gcagctcttc gaccaggagc
      421 gcgtagttgc ggctgatgag ctcggggtcg gtataggcga gcgcgaacgg ctcgatgtag
      481 atgcccttgc acacgcgcac gttggccttc accttggcca gcgcgcgcac gtcggccagg
      541 ctgcgccgca tgcgcgactg gatgaccacc cccacattgt cgaagtcggg acgaaggcgg
      601 tcgtagatct cgagcgtgtc gtcgatgcac ggcgagtctt ccatgtccag gcgcacgaag
      661 agcccctgcg cgcgcgcggc gaccaggagc gtgcgcacgt tctcgaggca gaacgcgacg
      721 tcgagtttga ggcccaggtg ggtcggcttg acgtggatgt agccgtcgat acggcgctcg
      781 cccatgctcg ccaggagcgc ctggtactcg tgcaccgctt gctcggattc ttcgcgccgg
      841 gtcacgaact cgcccaggat ggcggccgcc acgcggtagc cgtcgcgatt gagtccctcg
      901 gcgatgcgca cgaggtcgtc catcttctcg ccggcgatgt aggggctcgc gacgcgacgc
      961 accaccggct tggggaccag cggcagcgtc atggaaacga cgcggtcgaa aacacccatg
     1021 gatcgtcgaa tgactttcta gcggttagga cgtcggaacc ccatcgacac gacgggaccg
     1081 cattatactc gcaccagcga cggcggaaaa gcgattgttc ggcaccgcgc aacgctcgcg
     1141 tgcggtctac tccgaagccc cgaaggtcgg ggtcggcagc ggcaggctct gccccacttc
     1201 cagcacccgc aaccggtcgc cgatcacgcg cgcgagcgtc tcgccgtcgc gcggccgctt
     1261 gttacccccg aacacccagt atccgaatcc accccagtgg gcggggatca cgaccggcgc
     1321 gcgcgtggcc caggccgcgc gggtggcgtc ggtcgagggc aggtggccgt tgatgttgag
     1381 aatggcgata tcggggtcga aggtccaccc cacgtcgccg aagcccgaga agtagtcggt
     1441 gtcgccgctg tagaagatcg tcgccgcggc ggtctcgatc acgtagccga gcgcgcgccc
     1501 gtccgacgcc gcgtccagtc ccaagcgcgg tcccatgtgg tgggccgcca ccgcggtgac
     1561 acgcacgccg tcggtttcga agacgtcgcc gggcgccacc gcgcgcacct cgcgccccac
     1621 gtcgtccagg tacttcgcgc acgggcgcgg cgccaggatc accacgctct ccgggaacgt
     1681 gcgcagcgtc tccgcgctca agtggtccgg gtgcgcgtgc gatatcaata ccacgcgcgc
     1741 acccgcgtac gacgataccg gcggcgacgg gatgcggcgc tggcgaaaga tcaccgagcg
     1801 ctcgaacacg gggtcggtga ccacgcccac gccgcccgcg tcgatgtaca cgcacgcgtg
     1861 gccccagaac gtgaccgcat cgcgcggcgg cgccgggaac agctccgcgt tcggagccga
     1921 gagtcccccg cacccggcgc acgcgagcac gcacacggca atcaacacca tctcgcaacg
     1981 catcatagtc gcttccgtcg gaagatcacc acactcgccg tcaacagcac cgccgagatc
     2041 acggccagcg ccgccacttc gcgcaccaga tcgtcgaatc cagccccgcg gatgatgatg
     2101 ccgcgcgaga tcgccacgaa gtgggtggcc gggagcgcct ccgcgaccac acgcagcggc
     2161 accggcaacg acgacaacgg gaagatatac cccgagagca tgatcgaagg cagcagaatt
     2221 ccctgggcgg cctgggtcgc ttcgatttga gtggccgagc gcgccgagat catcagcccc
     2281 aatgcgagca gcgacgtcaa gtacaccaac gagagtcccg ccaggagcaa cacgctcccg
     2341 tggatcggca ctttgaaaaa gacgtgcatg caaaacagaa cgagcaccag ttgcacgaac
     2401 gccagcacca ggtacggcac cagcttcccg aggatcatct cgagcgacga gaccggcgtc
     2461 accatcagct gttcgagggt gccgcgctcg cgctcgcgca cgatcgcgta ggcggtgagc
     2521 agggtccccg agaacgtgag caggatcgcg atcagtcccg ggatcaggag gttcgcgctg
     2581 cgcgtgtccg gattgaagag aatctggggc cggggcgcga ccgggagctc gcccgcgccc
     2641 gtgcgcgcct gcaggtcctc cagcgatcgc gtgagcgccc agccgtcgat cgcggccagg
     2701 ctctgcgacg cgatggtgtt gtcggagccg tcgatgagcg cgaggaacgt cgcctgggtg
     2761 ccttcgaggc gccgcttggc gtagtcgggc gggatctcga tacccacctt cgcgtccccg
     2821 gccacgatgg cctcgcgcaa cgcgcttcgg ctcgtcaccg gacgctcgat ccggaaggtc
     2881 ttggtcgcct ccagctcgtc gaggagtgca cgactctcgg ggcagcggca ttggtcgaac
     2941 accgcggttg gcaccaaggt gacgttggtg tcgatggagc cgaacagaat cagctgaaag
     3001 accgggatgc cgagcgccag ccgcagcgtt cccttgtcgc gacgcatgtg gatcgcctct
     3061 ttgcgaagca ccgcgacgaa gcccttcatc gcgcggtctc cttggcggcg gtcacggcgt
     3121 cgtcctggag cgcggtcaag cgcacgaaca cgtcctcgag cgacggcatg atcggacgaa
     3181 tggcgacatc gacggggaca tccgatgaca ggtcccgctg tatcgcctcg tcggtgatgg
     3241 tcgtgggcac caggaggtgg aggctgttgc cgaagatggt gacctcgcgg acgtacgcga
     3301 gcgagcgggc gcgccgcatc acgggcgcgg ggttaccgtc gatggcggct tcgacgcggc
     3361 gcgtcccggc gggcgtgatt tccggcagcc tcaccaagtc ttcgggctcg ccctcgacca
     3421 gcaagcgcga gaggtacagg tagcccacgc ggctgcagcg ttcggcttcg tccatgtagt
     3481 gggtcgaaac caagagcgtg acgccgcccg cggacagatc gaacagcaaa ttccacagtt
     3541 cgcgccgcgc gaccgggtcg atgccggcgg tgggttcgtc gagaaacacg atgcgcggct
     3601 cgtgcatgag ggcggcgccg agcgaaaggc gttgcttcca tcctcccgag agctggcccg
     3661 cgaggcggtc gcgatagggc gcgatgcccg tcagcccgat cacccactcg cgccgctcga
     3721 ccaggcgctc ccccgagagc ccgtacacgc gcgcgaagaa attgaggttt tcgagcaccg
     3781 tcaggtcctc gtagagcgag aagcgctggg gcacgtagcc gatccgtttg cgaaccgcgt
     3841 cggcctcgcg cgtgacgtcg atgccgtcga cgaaggcgtg gcccgaggtg ggacgaagca
     3901 gtccgcacag aatgcgaatg agcgtggatt ttccggagcc gttgggcccc aacagtccga
     3961 agatggtgcc gcgcgcgatc gacagactca cgtcgcgcag cgcgtggacc ggaccgtagt
     4021 cgtgcgacac gccgcgcacc tcgatgccga agcgatcgtc ggcgcgttcc tcggcggggc
     4081 tcattcgagg cgaaccgtgg ccgtcatgcc cgccttgagc tcgggcgccg gctcgatgtc
     4141 gagcttaacg gcgaacacct ggtcttcgcg ctgctcgacc gtttgcacgt tgcgcggcgt
     4201 gtactcggcg cgagcgctca ccgaggcgac gcgcgccggg aacgcgcgct ctggaaacgt
     4261 gtcgatggtg acgtcgaccg actgcccgac ccgcacgcgc cccaagcgcg tctcgggcac
     4321 gaagacgcgg acccacaact cgtccggtgc caggatcgcg acgatcccct ggttggccgc
     4381 gaccatgtcg cccggccgca gatcgatggt ctgcacgacg ccggccgacg gcgagcgtac
     4441 ctcggtctcg gcgagctggc gctgcaagta gacgaggtga ctgcgggctt cgtcccaggc
     4501 ggcgcgcgcc tggcgttcgt cttcgctccg gcttccggcg cgaagctctt cgtagacttg
     4561 gaaggcggac tgggccgcgg cggccgccgc gtcgtaggct tcatccgagg tcagcgcttg
     4621 cgcgagcaag gactcctggc gcttgcgctc cttctcggcc cggtcccatt cgatgcgcgc
     4681 gcgctcgatc tgctcgcggc ggggcccgct cacgacgcgg tccagggccg cctgtgcttg
     4741 cgccacgcgg gcttcctgct ccctgacttg cgcgaccagc aggtcgccgt ccaggcgaac
     4801 caggacttgc ccggcctgca ccgagtcgcc ctcttcgacc aggacgtcca ggacgcgccc
     4861 gcccaccagc gaccccaccc gcgcgtcgcg cgcctcgatg gtgccggagt acgcgtggtc
     4921 ctcgccgcgc cctccgcgca gcacgagcca cacgaagata cctgcgacga gtacgactgg
     4981 gagaatgaga cggcgcatg
//
LOCUS       DASPGK010000318         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_664666, whole genome shotgun sequence.
ACCESSION   DASPGK010000318 DASPGK010000000
VERSION     DASPGK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664666"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1433)
                     /gene="hutI"
                     /locus_tag="VEC56_09010"
     CDS             complement(156..1433)
                     /gene="hutI"
                     /locus_tag="VEC56_09010"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXV14332.1"
                     /translation="MSTKQVSADLVVTGCGQLVTLAKPDRTPRVGPALRELAIVEDGA
                     LAVAAGRLISVGPRDEVLATTGVSAQTTHIDAGGAVVMPGFVDCHTHTVFAEYRLDEY
                     EWRVTGTPYAEIAARGGGIAKSVGHVRSTPEGVLLERTRKRVWSAISYGTTTIEIKSG
                     YGLSLEHELKQLRVVRALRDELPIGVVATFLGAHAIPKEMAAGREGYVQALIHEMIPH
                     VAAEGLAEFNDVFCEVGAFSREETERILRFGQRAGLKARIHADELTDMGGAELAASLG
                     AVSADHLKKANDKGIDRMAKAGVFGVLLPGTSFGLPSLELAPARKMVDAGMRLAIATD
                     FNPGSSTSESMPMMMSIACSHMRLSPAEALSMATYNPAFVLGREGDVGSLEVGKRADF
                     LVLDCRDYREVANHFGINPVARVFVAGTEWNAA"
     gene            complement(1452..>2620)
                     /gene="hutU"
                     /locus_tag="VEC56_09015"
     CDS             complement(1452..>2620)
                     /gene="hutU"
                     /locus_tag="VEC56_09015"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXV14333.1"
                     /translation="TGGLGGMGGAQPLAVTMNGGVVIAVEVDPSRIEKRLATRYLDVR
                     CDSVDEAVALATEATRAHKPLSIGLLGNAADVFPALLARGVEIDVVTDQTSAHDPLNG
                     YVPQGLDVAAAAELRARDPKGYVARAMASMVTHVEAMVGYQRAGSAVFDYGNNIRGQA
                     QTAGYADAFAFPGFVPAYVRPLFCEGYGPFRWIALSGDPKDIAVTDELALELFGERPA
                     LARWLSLARSRVQFQGLPARICWLKYGERARFGERLNDLVATGKVSAPVVIGRDHLDS
                     GSVASPYRETEAMRDGSDAVADWPILNALLNAVNGAAWVSVHHGGGVGMGLSIHAGLA
                     VVVDGTEEARAKAVRCLTGDPGMGVARHADAGYPEAIDAARRDSVRIPMLRDSQ"
BASE COUNT          426 a          917 c          838 g          439 t
ORIGIN      
        1 cgttgtgcac gttgggattg ttctggtcga gttcgaccag ccgccggtac tccttgatcg
       61 cgttcggcca atcctgatcg cgaacgaggt ccagggcctt cttgcgaatc gtcgttgcct
      121 tgctcacgtt gcttgccaac ttcatcgtcc ccgcgtcacg ccgcgttcca ctcggtgccg
      181 gccacgaata cgcgcgcgac cggattgatc ccgaagtggt tcgcgacctc tcggtagtcc
      241 cggcagtcca gcacgaggaa atcggcgcgc ttgccgacct cgagactgcc gacgtcgcct
      301 tcgcgcccga gcacgaaggc ggggttgtat gtcgccatcg acagcgcttc ggccggagag
      361 aggcgcatgt gcgagcacgc gatcgacatc atcatcggca tcgactccga ggtcgagctg
      421 ccgggattga aatcggtcgc gatggccaga cgcatcccgg cgtcgaccat cttgcgcgcg
      481 ggtgccaact ccagcgacgg aagcccgaag ctcgtccctg gaagcagcac gccgaaaact
      541 cccgccttgg ccatgcggtc gattcccttg tcgttcgctt tcttgaggtg gtcggccgag
      601 accgcgccga gggaggcggc aagctccgcg ccgcccatat cggtcaactc atcggcgtgt
      661 atcctggcct ttagtccagc ccgctgcccg aaccgcaaga ttcgttccgt ttcttccctg
      721 gagaacgccc cgacttcgca gaagacgtcg ttgaactcgg ccaggccctc ggcggccacg
      781 tgagggatca tctcgtggat gagggcctgg acgtacccct cgcgtccggc ggccatttcc
      841 ttgggtatgg cgtgggcacc caggaaggtc gccaccaccc cgatcggaag ctcgtcccgg
      901 agcgcgcgga ccacccggag ctgcttaagc tcatgctcca atgaaagtcc gtaaccgctt
      961 tttatttcta ttgttgtcgt tccgtacgag atcgcgctcc agacccgctt ccgggtccgc
     1021 tccaagagca cgccctccgg cgtgcttctc acgtggccga cgctttttgc gatgccgccg
     1081 ccgcgcgccg cgatctcggc atagggcgtg ccggtcacgc gccactcgta ctcatccagg
     1141 cggtactcgg caaagacggt atgggtgtgg cagtcgacga agcccggcat caccacggca
     1201 ccgccggcgt cgatgtgggt ggtctgcgcc gaaacccccg tggtggccag aacctcatcg
     1261 cgcggtccga ccgaaatcag ccggccggcc gcgaccgcca gggcaccgtc ctcgacgatg
     1321 gccagttcgc gcagcgccgg accgacgcgc ggagtgcggt cgggcttggc gagcgtcacc
     1381 aattggccgc agccggtgac gacgagatcc gcgctgacct gcttcgtgct catgtgggat
     1441 gtgaaacggc gctactggga atcgcgcagc atcgggatgc gaaccgaatc gcggcgcgcg
     1501 gcgtcgatgg cttccgggta accggcgtcg gcgtggcgcg cgacacccat gccggggtcg
     1561 ccggtcaggc atcgcaccgc tttggcgcgc gcttcttcgg tgccgtcgac caccaccgcg
     1621 agtccggcgt ggatcgacag ccccatcccg actccgccgc cgtggtgaac cgacacccag
     1681 gcggcaccgt tgaccgcgtt caagagcgcg ttcaagatcg gccagtcggc gaccgcgtcg
     1741 ctgccgtcgc gcatcgcctc ggtctcgcga tacggactcg cgaccgagcc gctgtcgagg
     1801 tgatcgcgcc cgatgacgac gggtgcactc accttccccg tcgcgaccag atcgttgagg
     1861 cgctcgccga aacgggcgcg ctcgccgtac ttgagccagc agatgcgcgc cggtagtccc
     1921 tggaactgca cgcgcgagcg cgccagcgat agccagcgcg ccagcgccgg cctttctcca
     1981 aacaactcaa gcgcgagttc atcggtgacc gcgatgtctt taggatcacc cgacaaggcg
     2041 atccaccgaa acggaccata cccctcacaa aaaaggggac gaacatacgc cggcacgaaa
     2101 ccgggaaacg cgaacgcgtc cgcgtagccc gccgtctgcg cttggccgcg gatgttgttg
     2161 ccgtagtcga acacggccga acccgcgcgc tggtatccca ccatcgcctc gacgtgcgtc
     2221 accatcgacg ccatcgcgcg cgcgacgtag cccttggggt cgcgcgcgcg cagctcggcc
     2281 gcggccgcca cgtccaggcc ttgcggcacg tagccgttga gcgggtcgtg ggccgaggtt
     2341 tggtcggtga cgacgtcgat ctcgacaccg cgcgcgagga gcgcgggaaa aacgtcggcc
     2401 gcgttgccga gcaacccgat cgatagcggc ttgtgcgcgc gcgtggcctc cgtggcgagc
     2461 gcgaccgcct cgtcgacgga atcgcagcgc acgtcgaggt agcgcgtggc gagacgcttc
     2521 tcgatgcgcg acggatcgac ctcgaccgcg atcaccacgc cgccgttcat cgtcaccgcg
     2581 agcggctgcg cgccacccat tccgcccagg ccgccggtca
//
LOCUS       DASPGK010000319         5743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1014608, whole genome shotgun sequence.
ACCESSION   DASPGK010000319 DASPGK010000000
VERSION     DASPGK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014608"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..2150)
                     /locus_tag="VEC56_09020"
     CDS             complement(81..2150)
                     /locus_tag="VEC56_09020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXV14334.1"
                     /translation="MNRIRTLALLFAVTGFGLAGVVAAKEAPPAPEPLKKLAFPAYSE
                     QKLKNGLEVVVVEHHEQPVASVWMAIKAGSVLDPEGKSSLASYTGSLLNKGTKTKDSK
                     QLAEWIESVGGSFNASTDEDGTILTISILSEYLPTAYEFLADIVLNPTFPEDELEEER
                     KRAVTGIEFEKSDPDAMADRHFHAVVYGNHPYAIRPTTETVEAVTRDDVVGFHKKNYV
                     ANNALMFVVGDVTAKQVKKDVETYFGKWTAGTPDVPNYAASPERTAKNIALYHRPGSV
                     QTNIQVGQLGLRPTDPDWPAVQVANRVLGGGATGRLFMSLREKHGWTYGAYSGWSKPK
                     DVGTFDAQANCRTEVTDSALTEMLAQIDRIVKEPVSPEDLASAKSYLVGNFPTTIETP
                     NQIAAQIGTVKLLGLDKSYLENYRKDVAKVTTADVQRVMQKHLHPDKLAIILVGDATA
                     IKDKVDPIASVALYDIEGNAVSMDELAVQGTDFDFDTSALKNMSATYAVKYQEMNLGD
                     MNVSLTRNSADFSSTSTITGMISMNEEMKFGQDFEPLAYKFSMAAGPQQMSADLSFAD
                     GVAKGRVEGGKDGGKDINVTLVKGAILKNAVDFLVGTLPLETGKTFKFPVLDTQSGGM
                     ENVTIEVVGEQDLMVPAGSYSTYKIKVKSGDGEQVFYVQKTSPHLLVKQENPAQGLNI
                     ELKSLKM"
     gene            complement(2188..3528)
                     /locus_tag="VEC56_09025"
     CDS             complement(2188..3528)
                     /locus_tag="VEC56_09025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV14335.1"
                     /translation="MKRLTWVVALIAVAAFTTAGYGKKDSKAGYIEYEEHTLPNGLRV
                     VLSEDHSVPVVAVNVWYHVGSANEEKGRSGFAHLFEHMMFQGSENVGKGDHMKYIAEA
                     GGSMNGSTTNDRTNYYQTLPSNRLNLGLWLEADRMQSLQVTSENFENQRETVKEERRQ
                     GIDNQPYGEAFLVSDTLGYDGWPYDHTVIGSMDDLDAAQVPDVQAFFDKYYCPANAVL
                     VVVGDIEPKKTMEMVKEYFGDIPAGEKAAFPKWNEPFNKGERRKVVDSPKANVPALFA
                     TYQIPGHLHADTPALELLNSLLVEGESSRLHKRLVKDEEAAFGAFGFVDGRIGPGQFR
                     VIVASNAGVDIETCEKLMDEEIEKVKTAGITDEELAKVKTQFKSDFIGQRQTVMNKAE
                     EIHHYIWFHGDLSVINTELDTYMKVTAEDIKRVANQYLRSDNRTLVVAAPPKSS"
     gene            complement(3884..>5743)
                     /locus_tag="VEC56_09030"
     CDS             complement(3884..>5743)
                     /locus_tag="VEC56_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXV14336.1"
                     /translation="CAQKGVKGLVIITAGFREIGPEGARLEDAIIGVAARHGMRMIGP
                     NCMGVIDATPDVRMNATFSPGVPPTGTVAFMTQSGALGVAILLAVQKLGLGFSYFASV
                     GNKADVSAMDLLEYWENDPHTQLIAMYLESFGDPRRFTEMSKRISKTKPIVVVKSGTS
                     AAGARAASSHTGSLAGLEVASEALLQQCGVNRVSSIEEMIHVVAGFVGAPMPRGNRLC
                     IVTNAGGPGIMAVDAADGHGLQIATLSETTRAAMRKVLPKEASVENPIDMVAGAGPDQ
                     YERVLDLAVADDNVDVAIAIFVPPLMIEPLEVMRRITRVARAHEKPVYSVLMAEESYY
                     ERIPREVPNAVPIYRFPEDAVKVAEHTNRYRLWRARPAGTVRTFPVDAASARALVEKK
                     ARAGGGYLAPEETHAVLGAYGFPTVGHAVVAVDGDLRAAASALAFPLVLKVVGEKIVH
                     KSDVGGVILHIRDDKALERARETMRASLAKAGVLAHATGFLVQEMAGDANGKEVILGV
                     AQDPKFGPLLMFGMGGRYVEILRDVAFRVLPITDVDAHDMVRSIRSFPMLEGVRGEAA
                     VDVPFVEEMILRLAQLIDEVPGIQELDMNPVIVTPTRAGCRVVDARIRASASP"
BASE COUNT          902 a         1861 c         1858 g         1122 t
ORIGIN      
        1 cgaacgggtc caagaggtgg gcaactttcc ccggcggaag ggcgcgggcg agcgccgaaa
       61 ggcgcccgcc cgccggtgcg ttacatcttc aggctcttga gctcgatgtt gagaccctgt
      121 gccggattct cctgcttgac cagcaggtgg ggcgaggtct tttgcacgta gaagacctgc
      181 tcgccatccc cgctcttcac cttgatcttg taggtcgagt aggagccagc cggcaccatc
      241 aggtcctgct ccccgaccac ctcgatggtc acgttctcca tcccgcccga ctgggtgtcc
      301 agcaccggga acttgaacgt cttgcccgtc tcgaggggca gggtaccgac caggaagtcg
      361 accgcgttct tgaggatggc gcccttgacc agggtgacgt tgatgtcctt gccgccgtcc
      421 ttgccgcctt cgacgcgtcc cttggccaca ccgtccgcga acgacaggtc ggccgacatt
      481 tgctgcggac ccgccgccat cgagaacttg tacgccagcg gctcgaaatc ctgaccgaac
      541 ttcatctctt cgttcatgga gatcatgccg gtgatggtgg aggtcgacga gaagtcggcc
      601 gaatttctgg tcagcgacac gttcatgtcg ccgagattca tctcctggta cttcacggcg
      661 taggtggccg acatgttctt gagcgccgac gtatcgaagt cgaagtcggt gccctgaacc
      721 gccagctcgt ccatcgagac cgcgttgccc tcgatgtcgt agagcgccac cgacgcgatc
      781 gggtcgacct tgtccttgat ggcggtggca tcgccgacca ggatgatcgc gagcttgtcg
      841 ggatgcaggt gcttctgcat cacgcgctgc acgtcagccg tggttacctt ggccacgtcc
      901 ttgcggtagt tctccaggta gctcttgtcg agcccgagca gcttgaccgt accgatctgg
      961 gccgcgattt ggttcggcgt ttcgatggtc gtgggaaagt tgccgaccag gtacgatttg
     1021 gcgctcgcga gatcttcggg gctcacgggc tccttcacga tgcggtcgat ctgcgccagc
     1081 atctcggtca gcgcactgtc ggtgacttcg gtacggcaat tcgcctgcgc gtcgaaggtg
     1141 cccacgtcct tgggcttgct ccagcccgag taggcgccgt aggtccagcc gtgcttttcg
     1201 cgcagcgaca tgaacaaacg gccggtagca ccgccgccca ggacgcggtt cgcgacctgg
     1261 accgccggcc aatccgggtc ggtcgggcgc agtcccagct gccccacctg gatgttggtc
     1321 tgcacggaac cggggcgatg atagagcgcg atgttcttgg ccgtgcgctc gggcgaggcg
     1381 gcgtagttgg ggacatcggg cgtgcccgcg gtccacttcc cgaagtacgt ctcgacgtcc
     1441 ttcttgacct gcttcgcggt gacgtcaccc acgacgaaca tgagcgcgtt gttggcgacg
     1501 tagttcttct tgtggaaacc caccacgtcg tcgcgcgtca ccgcttcgac ggtttccgta
     1561 gtcggacgaa tcgcgtacgg atggttgccg tacacgaccg cgtggaagtg gcgatcggcc
     1621 atggcgtccg ggtcggattt ctcgaactcg atgccggtga ccgcgcgctt gcgctcttcc
     1681 tccaactcat cctcggggaa ggtcggattc agaacgatgt ccgcgaggaa ttcatacgcg
     1741 gtgggaaggt actcgctcaa gatcgagatg gtcaggatgg tcccgtcctc gtcggtcgag
     1801 gcgttgaacg aacctcccac cgactcgatc cactccgcga gctgcttcga atccttggtc
     1861 ttggtgccct tgttgagaag cgagcccgtg tacgacgcga gcgacgactt cccttccggg
     1921 tccagcaccg agccggcctt gatggccatc cataccgacg ccaccggctg ttcgtggtgc
     1981 tcgaccacga ccacctcgag gccgttcttg agcttctgct cgctgtacgc gggaaacgcg
     2041 agtttcttga gcggctcggg cgccgggggc gcttccttcg ccgcgacgac gccggcgagc
     2101 ccgaacccag tcaccgcgaa gagcagcgcc agtgtacgaa tgcgattcat gtcggtttgt
     2161 cctccttgag cgattctctt cgggtggtta gctgctcttg ggcggcgctg cgacgaccag
     2221 ggtacggttg tcggaacgca ggtattggtt cgccacgcgc ttgatgtctt ccgcggtgac
     2281 cttcatgtag gtgtcgagct cggtattgat gaccgagagg tcaccgtgga accagatgta
     2341 gtggtggatc tcttcggcct tgttcatcac cgtctggcgc tggccgatga agtccgactt
     2401 gaattgcgtc ttcaccttgg cgagctcttc gtccgtgatg cccgcggtct tgaccttctc
     2461 gatctcttcg tccatgagct tttcgcaggt ctcgatgtcg acgccggcgt tcgacgcgac
     2521 gatgacgcgg aactggcccg ggccgatgcg cccgtccacg aagccgaacg cgccgaaggc
     2581 ggcctcttcg tccttgacca ggcgcttgtg cagccggctc gactcgcctt cgaccagcaa
     2641 gctgttcaag agctcgagcg cgggcgtgtc cgcgtgcaga tgaccgggaa tctgataggt
     2701 ggcaaacagc gccggcacgt tggccttggg cgaatcgacc accttgcggc gctcgccctt
     2761 gttgaagggc tcgttccact tcgggaacgc ggccttttcg cccgcgggga tgtcgccgaa
     2821 gtactccttc accatctcca tcgtcttctt gggctcgatg tcgcccacca cgaccagcac
     2881 cgcgttcgcg gggcagtagt acttgtcgaa gaaggcctgc acgtcgggca cctgggcggc
     2941 gtccaggtcg tccatgctgc caatcacggt gtggtcatag ggccagccgt cgtaaccgag
     3001 cgtgtcggac acgaggaagg cttcgccgta cggttgattg tcgatgccct ggcgacgctc
     3061 ttccttgacc gtctcgcgct ggttctcgaa gttctcgctc gtcacctgga gcgactgcat
     3121 gcggtcggcc tcgagccaca ggccgaggtt caagcggttg gagggaagcg tctggtagta
     3181 gttggtgcgg tcgttggtgg tcgagccgtt catcgagccg ccggcttcgg cgatatattt
     3241 catgtggtcg cccttgccga cgttttcgga accctggaac atcatgtgct cgaacaaatg
     3301 cgcgaacccg ctgcgaccct tctcttcgtt ggcgctgccg acgtggtacc acacgttgac
     3361 ggccaccacg ggcacggagt gatcttcgga caggacgacg cggagcccgt tgggaagggt
     3421 gtgctcctcg tactcgatgt aaccggcctt gctatccttc ttgccgtatc cggccgtggt
     3481 aaacgccgct acggcgatga gtgccacgac ccatgtaagg cgcttcatgc ctggataacc
     3541 tccactgatt gtttttcgaa tcacgccccg atttccgttc atgaggagag gcgggttggg
     3601 tgcgcgggtt ggttccggag cgacctatac ccgatagcgc cccccgcgtc aaccagccgg
     3661 ggggcgtgcc ggggcgtaag tgactctgaa gtcgtcttac ggcgcgaacg ccgagggggt
     3721 ttcaactttc acgcgcgggg tgcgcgggcg ttgtcccggc gcgctccgag gcgcgcgcgg
     3781 ctcgacaccc cgggacagcc ggcggcccgg acgaccgccg ctgacgcata acccaatatc
     3841 ccacaaatga ttacaccacc gaaggcggtg ccgcgggtcg gccctagggc gaggcagagg
     3901 cgcggatgcg cgcgtcgacg acgcgacagc ccgcgcgcgt gggtgtgacg atgacggggt
     3961 tcatgtcgag ctcctggatg cccgggacct catcgatcag ctgcgccagg cggagaatca
     4021 tctcttcgac gaaggggacg tcgacggccg cttcgccgcg cacgccttcg agcatcggga
     4081 acgagcgaat gctgcgcacc atgtcgtgcg cgtcgacgtc ggtgatgggc agcacgcgaa
     4141 acgcgacgtc gcgcagaatc tcgacgtagc gcccgcccat cccgaacatg agcagggggc
     4201 cgaacttggg gtcctgcgcg acacccagga tcacttcctt gccgttcgcg tcgcccgcca
     4261 tctcctgcac gaggaagccg gtcgcgtgcg cgagtacgcc tgctttggcg agcgaggcgc
     4321 gcatggtctc tcgcgcgcgc tcgagtgctt tgtcgtcgcg gatgtggagg atcacgccgc
     4381 ccacgtcgga cttgtggacg atcttctcgc ccaccacctt gagcacgagc ggaaatgcga
     4441 gcgcgctcgc cgcggcacgg aggtcgccat cgaccgcgac caccgcgtgg ccgacggtcg
     4501 ggaatccgta cgcgccgagc accgcgtgcg tctcctccgg cgcgagatag ccgccgccgg
     4561 cgcgtgcttt cttctcgacc agagcccgag cgctcgccgc atcgaccggg aacgtgcgca
     4621 ccgttcccgc cggccgcgcg cgccacaggc gatagcgatt ggtgtgctcg gccaccttga
     4681 ccgcgtcctc ggggaatcga tagatgggga ccgcgttggg cacctcgcgc gggatgcgct
     4741 cgtagtagct ctcctccgcc atcagcaccg agtacaccgg cttctcgtgc gcacgcgcca
     4801 cccgcgtgat ccggcgcatc acttccaagg gctcgatcat caagggcggc acgaagatgg
     4861 cgatggcgac gtcgacgttg tcgtcggcga cggccagatc cagcacacgc tcgtactggt
     4921 cggggccggc gcccgccacc atgtcgatgg gattctcgac gctggcttcc ttgggcagca
     4981 cctttcgcat ggcggcgcgc gtcgtctcgg agagcgtcgc gatctggaga ccgtggccgt
     5041 cggcggcgtc gaccgccatg atgccggggc cgcccgcgtt ggtgacgatg cacaggcggt
     5101 tgccgcgcgg catgggtgct cccacgaagc ccgccaccac gtggatcatc tcttcgatac
     5161 tcgacacgcg gttgacgccg cactgctgca ggagcgcttc cgacgccacc tcgagccccg
     5221 ccagcgaccc ggtgtgcgac gacgccgcgc gcgcgcccgc cgccgaggtc cccgacttca
     5281 ccaccacgat cggtttggtc ttcgagatac gcttggacat ctcggtgaag cggcgcgggt
     5341 cgccgaagga ttccagatac atcgcgatca gctgcgtgtg ggggtcgttc tcccagtact
     5401 cgagcaggtc catggcactc acgtcggcct tgttgccgac actcgcgaag tacgagaagc
     5461 cgagcccgag cttttgcacc gcgagcagaa tcgcgacccc gagcgcaccg ctctgggtca
     5521 tgaacgccac cgtgccggtc ggcggcacgc ccggcgagaa ggtcgcgttc atgcgcacgt
     5581 ccggggtggc gtcgatgacg cccatgcagt tggggccgat catgcgcatg ccgtgtcgcg
     5641 cggccacacc gatgatggcg tcttcgagcc tggccccctc ggggccgatc tcgcggaagc
     5701 cggcggtgat gatgacgagt cccttgacac ccttctgcgc gca
//
LOCUS       DASPGK010000320        16070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1249990, whole genome shotgun sequence.
ACCESSION   DASPGK010000320 DASPGK010000000
VERSION     DASPGK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249990"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VEC56_09035"
     CDS             <1..824
                     /locus_tag="VEC56_09035"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV14337.1"
                     /translation="MPRAEIDRRVTEAARTLELDALLDRKPAQLSGGQRQRVALGRAI
                     VREPRVFLYDEPLSNLDARLRTDMRATIRRLYDRLRTTSIYVTHDQVEAMTIGDVLVV
                     MNKGRVHQVGSPEACYREPADTFVASFLGSPAMNFADGRLDGDAVVIDGGTRLALPQP
                     VARAASSRPGAEVRLGVRPEDLRPGSGEVANAARLSGMVALREPLGHETLTHLQVGGA
                     DWVARGTHEFTPDSSGRTTVFLDLDRMHIFWKDSGRRVGSAGLAAAARGTTEAPA"
     gene            821..1636
                     /locus_tag="VEC56_09040"
     CDS             821..1636
                     /locus_tag="VEC56_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14338.1"
                     /translation="MSALIPWLNRVFRRPNVAGRESKEAYSRWEHHWGREFARLFMEP
                     AGDLAGKRLLDVGCGLGGKTVAYAEAGARAVGTDLVESNAVQSRAYAEQAKKGIEFFV
                     GDAGALPVRDGAFDTVVANDAMEHFSEPERALSEMVRVLRPGGAIWVFFTPHYSPLGS
                     HLYDYVYTPWCHLLFTRAQLSRGIRAVLVERGQSADTIDARVHAIMQSFDRDLNHMSI
                     RRFLNIVKGTPGVRVSRLDLKPAKFGALKPLTRVPGLRELVTGFVTCRLERIS"
     gene            1633..2580
                     /locus_tag="VEC56_09045"
     CDS             1633..2580
                     /locus_tag="VEC56_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HXV14339.1"
                     /translation="MSGVASLLTAMRVPEERDRTLLWAVIISIMIHASLLGFGLYQRQ
                     RGLEIDLTRAQEERDLPVEVALVEPPLRPIPTERTSTNLSTVSSEASDLDPRDTQSDV
                     PSGQQLLEQTPPGGSGAEQRAEPGEEASGTEREKELPQEQEGLSEALRQSRQGTTLDA
                     PHDFALPAERPDFGDDGSASMTIGNITLNTTAWDFAPYLLDLKRRIRQKWIPPMAFAQ
                     LGAIHGYTWVNFRIYPDGRMTELAVVETEGHDSLHRSSENAIRGAAPFRPLPDHFPED
                     YLEITFGFYYLLPGDELRYFRDGRPVRKDERARDDEGSP"
     gene            2612..3793
                     /locus_tag="VEC56_09050"
     CDS             2612..3793
                     /locus_tag="VEC56_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14340.1"
                     /translation="MIPIQIRRWATRVGRGAGFACVLAAFVIAACSSDPISTLGSDSE
                     LINSKPGVIVQDTLELFADTVLTYYTALAADTVLEFGRVSGYQRSMVIQNPFSTASSH
                     AAKVVSSAVLRLTASDVDGTFPARFYRLNDTYAEGDSIPTLDTLAVIDDPDTGSPNRT
                     LQTIPRDYPLPPALVQGWIRDEIERTALAVIYNDDVNDKIATFKARQNADSDRYRPQI
                     FVNFVGGTSATFRCSADATFVRPTTATSNLVVSDGFVRRIHLRIPLDQLPERSAVHNA
                     RVRLYLVPGSTLGSNPNLVVFIPASDDPTSTEFLTGQLVTATSFQASADYVEFAMTNA
                     IALILQGTLANNGIVVRFDAENSELRQVQFYGSSAPDSLRPRVFITSSTPADFDPPRG
                     P"
     gene            3790..4998
                     /locus_tag="VEC56_09055"
     CDS             3790..4998
                     /locus_tag="VEC56_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14341.1"
                     /translation="MMARVSGWVPAAVALAVLLGAVPTPRAESIFGMNLLGERLDAVD
                     ARTAALGGFVQTIDDSLGILQYNPAMLAWAKRVTFSASGYLTRDANQSPDLERTVVAS
                     KFSALGLAFPLFRKTLTASVGYRGRYDPDGDFSVPGETPAGETYNDIFARSGGTWSVP
                     ITMAADLGRFMKVGGTFSLERGTIENRWLIDFAGTTTADASSEQIREVRGTGFAGGVV
                     VRPIASVSVGVAYEAEIDYDVNVKESFTNASADTSYEETMRQPERWTASASWRVMRGF
                     TVYAGGSLCDFTNFEGLDFPSERLTEERVASFGLEYRFRGSRFPIRASARFEQLPYTM
                     PDGEEISRVAFTLGSGLIFRTGRGKLDAALTFAQVGSVDTNTYEDQQVRFFISIAGSE
                     SWATKREGRY"
     gene            4998..5711
                     /locus_tag="VEC56_09060"
     CDS             4998..5711
                     /locus_tag="VEC56_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14342.1"
                     /translation="MRHRAANTALRAGGVIALALAAACGQGKTAGKSPAPAPTLAQQL
                     STMEPEQQLAFLRGLEREKPGDATVAFHLGNAYYSLGSAVPAEENARAVAYYDSAVAE
                     YTRAVEIDSTYSKAYVNMGLAYDAATKRPDARRVLRKAIEVNPNDVLAYCHLGQIEQS
                     YGDYAEAVRLYQKALVLDPNSAQAHYNLGLAFAEKKLFKEALIEWERVIVLDPNGELG
                     KTATENVRIIRQYLEAAPK"
     gene            5713..6822
                     /gene="mnmA"
                     /locus_tag="VEC56_09065"
     CDS             5713..6822
                     /gene="mnmA"
                     /locus_tag="VEC56_09065"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXV14343.1"
                     /translation="MNGSFVTMKEARAGARVLLAMSGGVDSAVSALALVEAGYRVVGM
                     TMKNYCYGDAAVPERSCCSMEAIDDARAVCQRIGIRHLVVSTEELFGREVYHNFLGEY
                     RRGRTPNPCVRCNSIVRFDTLVEWADRMGFDHVATGHYSRVFRSTTGRRYIARARSRA
                     KDQSYFLSALDPRTLDRVLFPLGDREKDEVRADARDQGLRVADKPDSQEVCFVGTRTL
                     REFLDGKVEMTPGDVMTTAGDVVGRHEGLATYTIGQRRGLGIAAAKPKYVVALDPARN
                     TVVLGDDEDLYHSELSCDLAWIDADAARAASPLSAQIRSRHSAEPVASLAVEGDTARA
                     VFHAPQRAIAPGQTIAFYDGDVVVGAGVIAASGPA"
     gene            6833..7717
                     /locus_tag="VEC56_09070"
     CDS             6833..7717
                     /locus_tag="VEC56_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14344.1"
                     /translation="MLPRIRAIALALLLTALAAPSFANVRSLDPYPVTIHYQPRYSRV
                     AEKVDAIARAELPRLMSELGLVQLSPIEIVVTGDARDYDASLTHRLPNWGVAFAILDE
                     QRILVDVQKATRAYNSLDEVVPHELSHLLVRQCVPNAQLPIWFSEGLAQWQAREWSLF
                     DSWQLMQGVWTGSVPPIEDLGRQYPADEARAQDAYRISYAAFTDLFSEVGFAKLPEFL
                     GAVEAKESFAVGFADYWGFTVADYAVYFQDDVEKRYRSKALAFQSGPLFGFAALVFVA
                     VIIRQSIKRRRKFAQLEE"
     gene            7801..7888
                     /locus_tag="VEC56_09075"
     tRNA            7801..7888
                     /locus_tag="VEC56_09075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7834..7836,aa:Ser,seq:tga)
     gene            7933..8027
                     /locus_tag="VEC56_09080"
     tRNA            7933..8027
                     /locus_tag="VEC56_09080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7968..7970,aa:Ser,seq:gct)
     gene            8078..8452
                     /locus_tag="VEC56_09085"
     CDS             8078..8452
                     /locus_tag="VEC56_09085"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HXV14345.1"
                     /translation="MLMKSAVIKRVDLWSLFKMAFVLYATIGFIGGLFYGMIFAFIGS
                     LGAVLGEDMPGLGAVSGAIGIIVVPVLAIMTGIMGSVVVTIIGAVYNLAARFVGGVKV
                     TVDVAEIGPSAGYATTASPPAS"
     gene            8471..8547
                     /locus_tag="VEC56_09090"
     tRNA            8471..8547
                     /locus_tag="VEC56_09090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8505..8507,aa:Arg,seq:acg)
     gene            8736..9932
                     /locus_tag="VEC56_09095"
     CDS             8736..9932
                     /locus_tag="VEC56_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV14346.1"
                     /translation="MKKPSRKTLLVSLIAVVVIGAGLAFVAKTRMGIKNSASADSTVT
                     SDSTQAADATDKDKKKDKKKKKGEKVDAPVPVEVTTVAPRSISTYYQTTATLEPEKRV
                     DILSKLAGEVRQILVEEGDFVKEGAILCRLDDAELKVTLEEARINREQQKLEFERVKD
                     MQTQSLISDKEYQQIKYNYEVAENRYQSARVRYEYTQIKAPFSGVVTQRLVDPGENIP
                     MGAKLFMMSDTQPLLLSMYLPESEARLVRKGQRVAIRPDQGAEAEFEGEVLRIAPEVD
                     QRTGTVKVTAQTRAGGVPGSFVRVRILMDTHADVLAVPRRSLLSDAGDRFVFIAAADT
                     VRKVQVGIGYEDETHAEVTEGLAKGDTVVTAGVGGIRDGTKVKVVRPDDVADKDKETA
                     SNGEKK"
     gene            9962..13147
                     /locus_tag="VEC56_09100"
     CDS             9962..13147
                     /locus_tag="VEC56_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV14347.1"
                     /translation="MRIVDFSIQRPVTVTMIFVAVIVFGFVALTRLDVRLLPEISYPS
                     LTIQTEYEDAAPAEVENFVTRPLEEAVGVISGLRTIRSVSKPGMSEITLEFTWKTHMQ
                     YAALDVRDKIDLVLLPRETKAPVILRYDPSLDPVMRIGVSGTENLVRLRNLADYTIKK
                     DLESLDGVASAKVLGGLEEEIQVEIDERRLASYGIPISLVAARLAQDNVNQSGGRLRD
                     KGAEFLLRTENEFQSVEDIANTIVREEAGRRVILADLGSVTRGHVERDVITRLNGEEV
                     VEIAIYKEGDANTVAVADGVKRRLNAMKRQLPSDVTIETLFDQSRFIKSSIDEVRSNA
                     MVGALLAILVLYVFLRDVRSTVIIGLSIPISILFTFVIMQQLGVALNMMSLGGLALGV
                     GMLVDNSIVVLESISRHKEREPDRKLATLRGAKEVAGAVTGSTLTTVAVFLPIVFVEG
                     IAGQVFKDQALTVSISLVASLIVSLMLIPIASAMDFGASATAVAEPDAPVVPRRRIAR
                     AARSIAFFLTVGAPTFVLRTCRRALAWVGRGLAVLVRPLHLVYDRAFPAMESAYARVL
                     ERALSRRRRFLTGVFAVFVAAVALSPLVGREMIPQFSQGEFSFAVQMPEGTPLEATDA
                     RIVDMEAIAAAEPGVESYFTSVGTASRLGSNVKSKDENIGQVNVIMSEKGNKHAEAAL
                     VESLRAKFERVQGVTTKFARPSYFNFQTPLEVHVFGYDLDEIRDFADRLALDMADIPG
                     VKDVKSSLEYGNPELDVEFDRVRMSSMGLAVEEVANTVRTKIRGDVPTKFKERDKQVD
                     IRVRTSAWRAEDVAAMRSLVVAERDGTPILLGSIADVRVATGVNQISRVSQQRAAIVS
                     GNISGRDLGRVARDVEAVLASASIPEGITVELGGENEELMRSYRSLGLALILAVFLVY
                     LVMAAQFESFLHPFIIMFTVPLAAIGVVATLLLTSTPLSVIVFIGIILLAGIVVNNGI
                     VLIDYINTLRRDGKSKLDAIRSAGRVRFRPILMTTLTTVLGLLPMALGLGEGAEIRAP
                     MALTVIGGLSVGTLLTLLVIPSLYAVIARGK"
     gene            13149..>16070
                     /locus_tag="VEC56_09105"
     CDS             13149..>16070
                     /locus_tag="VEC56_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV14348.1"
                     /translation="MRLHELSVRKPVLVMIGLVTLVAMGGIATFKLPIEFLPHVEFPF
                     IGVYIPYANSHPDYIERHIIKPVEEVFATLGDVRRIQSQTQPDGAFIGVEFQWGRDVG
                     VLRMEVKEKLDQIKGELPADIVHMQIFTFDTNDIPILEGRISAGGRDLSGSYDLIERS
                     IINPLKRIEGVGNVFIHGVEPREIAIYLSLDKIKAHRVDVGNVFRLLQSANATASGGE
                     ITAHGVRYSVRSLGSFNDIDDVENLVVSPEGLRLRDIADIYYGEPAVAYGRRLNGEAA
                     VAFWIQKASNANTVDVARRVHAAMEKMNDDPALEGIDVLLFFDQSKEILNGVSGLRNA
                     GFQGGLLAIGILYFFLRRVTTTLIIAIAIPFSLVCTLAFLYFTGYTLNMLTMMGLMLG
                     VGMLVDNAIVVLESIYQHQLRREDPMHAAVTGADSVAVAVTASTLTSVIVFAPIVFGS
                     KNDELFVWLSSVGVTISVAILFSLLLSLTMIPFLASRLKRPKDVRPSRFLKSVENHYV
                     RALEWATFKHRKWTLGIVAGSLLLTVVAVKVIGLKGFQDDDSFIIERIFVEYDFAENV
                     DYKETNQFVRRVEAALEAKRDSLGIETIYSYYEDNRAMTTVYFEQERLSRDILKEKRK
                     ALRAAIPEMAGVKLRFGDESGQDSGGASLMQVNLFGEDKQVLEEIANEVKRRFGYLED
                     LTDVKTSVELGQEEISVAVDADLLARAQMTANDVVQVMGLTFRGFELNRFRGEDREVP
                     MSISLDPTDQVGIYNLHNLMVGMNDDREVTLGSVAEFEQKRGPTQIERDNQRTIVSVR
                     GLYEGERFDELKEEVGAVMRSLALPPGYAWSFGRRIEERDAQASQMLVNALLAILCVY
                     LMMAALFESYLHPLVIMICLPLAGIGVVWMLMLTGTAFSLMAMIGTVILIGVVVNNGI
                     VLIDHVNHLRKEGMPMEQAILEGGRERLRPILMTALTTILGLSPMAIGGSHIGEAQYY
                     PL"
BASE COUNT         2759 a         5241 c         5285 g         2785 t
ORIGIN      
        1 agatgccgcg cgcggaaatc gatcgccgcg tgactgaggc ggcgcgcacc ctcgaattgg
       61 acgctctcct cgatcgcaag cccgcgcagc tctcgggcgg gcagcgacaa cgcgtggccc
      121 tcgggcgcgc catcgtgcgc gagccgcgcg tgttcctcta cgacgagccc ctgtccaatt
      181 tggatgcccg gcttcgtacc gacatgcgcg cgaccattcg ccgcctctac gatcgtttgc
      241 gcacgacgtc gatctacgtg acgcacgacc aagtggaggc catgacgatc ggcgacgtgc
      301 tggtggtgat gaacaagggc cgcgtccacc aggtggggag cccggaagcc tgctaccgcg
      361 agccggcgga taccttcgtg gcgtcgttct tgggcagccc ggccatgaac ttcgcggacg
      421 ggcgcctcga cggcgacgcg gtcgtgatcg acgggggcac ccggctggcg ctgccgcagc
      481 ccgtcgcgcg cgccgcgtcg tcgcgcccgg gcgccgaggt ccgcctgggc gttcgccccg
      541 aggacctgcg accggggtcg ggggaggtgg cgaacgccgc gcgcctctct gggatggtgg
      601 ccctgcggga gccgttgggg cacgagacgt tgacccatct ccaggtcggg ggcgccgact
      661 gggtggcgcg cgggacgcac gagttcaccc cggactcgag cggccggact acggtgtttc
      721 tcgacctcga tcgaatgcat atcttctgga aggactcggg gcggcgcgtc gggtccgccg
      781 gccttgccgc ggcggcgcgc ggtacgaccg aagcgcccgc atgagcgcgc tcattccttg
      841 gctcaaccgc gtgtttcgcc ggcccaacgt ggcgggccgc gaatccaagg aagcctactc
      901 gcgctgggag caccactggg gccgcgagtt cgcccgactc ttcatggaac cggcgggcga
      961 cttggcgggt aagcggttac tggacgtcgg ttgcggcctg ggcggaaaga ccgtcgcgta
     1021 cgccgaagcc ggggcgcgcg cggtcggaac cgacctcgtg gaatcgaacg cggtccaatc
     1081 gcgcgcctac gcggagcaag ccaagaaggg gatcgagttc ttcgtcggcg acgcgggggc
     1141 actcccggtc cgcgacggcg ccttcgacac cgtggtcgcc aacgacgcca tggagcattt
     1201 ctccgaaccg gagcgggcgc tttcggaaat ggttcgcgtg ctgcgcccgg gaggtgcgat
     1261 ctgggtgttc ttcacacccc actattcgcc gctgggttcg cacctctatg attacgtgta
     1321 caccccctgg tgccatttgc tgttcacgcg cgcccagttg tcgcgcggca tccgcgcggt
     1381 cttggtcgag cgcggccaga gcgcggatac gatcgacgcc cgcgtgcacg ccatcatgca
     1441 gtcgttcgac cgtgatctga atcacatgag cattcgtcga ttcttgaaca tcgtgaaggg
     1501 cacgcccggc gtgcgcgtct cgcgcctgga cttgaagccc gcgaagttcg gggcactcaa
     1561 gccgctgacg cgcgtgcccg ggctgcgtga gctcgtgacc ggcttcgtga cctgccggtt
     1621 ggagcggata tcgtgagcgg cgtcgcctcg ttgttgaccg cgatgcgggt ccccgaggag
     1681 cgcgaccgca ccctgctgtg ggcggtcatc atttccataa tgatccacgc gtcgctgctc
     1741 gggttcgggt tgtaccagcg ccagcgcggc ctggaaatcg atctgacgcg tgcgcaagag
     1801 gagcgcgacc tgccggtcga ggtggcgctg gtcgagccgc ccttgcgtcc cattccgacc
     1861 gagcggacca gcaccaactt gtcgacggtt tcgtcggagg cgagcgacct cgacccgcgc
     1921 gacacccagt ccgatgtgcc gagcggacag cagttgctgg agcagactcc gcccggcggc
     1981 agcggcgcgg agcaacgcgc ggaaccaggc gaagaggcga gcggcaccga gcgcgaaaag
     2041 gagttgccgc aagagcaaga aggattgtcg gaagcgctgc gccagtcgcg ccaaggcacg
     2101 actctggatg cgccgcacga cttcgcgctt cccgccgagc gccccgactt cggcgacgac
     2161 gggtcggcga gcatgaccat cggcaacatc acgctcaaca cgacggcgtg ggacttcgcg
     2221 ccctacttgc tcgacttgaa gcggcgcatc cggcagaaat ggatcccacc catggccttc
     2281 gcgcagttgg gcgcgattca cggctacacg tgggtgaatt ttcggattta tccggacggc
     2341 cgcatgacgg aactcgcggt ggtcgaaacc gagggacacg actcgctgca ccgctcgagc
     2401 gagaacgcga tccggggcgc ggcgccgttt cgacccctgc cggatcattt cccagaggat
     2461 tatctcgaga tcacgttcgg cttctactac ttgctcccgg gcgacgaact gcgctacttc
     2521 cgcgacggcc gaccggttcg caaggacgag cgcgcgcgcg acgacgaggg ctcgccttga
     2581 atcttccccg gtcgtgtgct acaaggtgcc gatgattccc atccagattc gccgttgggc
     2641 gacgcgcgtg gggcgcgggg ccgggttcgc gtgcgtgctg gccgcgttcg ttatcgcggc
     2701 ttgctcgtcg gacccgatct ccacgctggg ctcggacagc gaactcatca acagcaagcc
     2761 gggcgtgatc gttcaagaca cgctggagtt gttcgccgat acggtgctca cgtactacac
     2821 ggcgctggcc gcggacacgg tcctggaatt cgggcgcgtg tcgggctacc agcgttcgat
     2881 ggtgatccag aatccgttct cgaccgcgtc ctcgcacgcc gccaaggtgg tgtccagcgc
     2941 ggtgctgcga ctgaccgcga gtgacgtcga cggcacgttt ccggcgcgct tctatagatt
     3001 gaacgatacc tacgccgagg gtgattcgat tccgaccctc gacacgctgg cggtgatcga
     3061 cgaccccgat accggcagcc ccaatcgaac gttgcagacc atcccgcgcg attacccgct
     3121 tccgcccgcg ctggtgcagg gctggattcg cgacgagatc gagcgtaccg cgctggccgt
     3181 gatctacaac gatgacgtca acgacaagat cgcgaccttc aaggcgaggc agaacgccga
     3241 ttccgatcgg tatcggccgc agatcttcgt caatttcgtc ggcggcacct cggccacgtt
     3301 tcgctgcagc gccgacgcca ccttcgttcg ccccaccacc gcgacttcca acttggtggt
     3361 ctccgacggc ttcgtgcgcc gtatccactt gcgcatcccg ctcgaccagc tccccgagcg
     3421 ttccgcggtc cacaatgcgc gcgtgcgcct ctatttggtg cccggctcga cgctgggcag
     3481 caaccccaac ctggtcgtct tcattccggc gtcggacgac cccaccagca cggagtttct
     3541 caccggtcag ctggtgaccg cgacgtcgtt ccaggccagc gccgactatg tcgaattcgc
     3601 gatgaccaac gcgatcgcgc tgattttgca gggtacgctc gcgaacaacg gaatcgtggt
     3661 tcgcttcgac gcggagaatt ccgagttacg ccaggtgcaa ttctacggct ccagcgcgcc
     3721 cgacagcctg cgcccgcgcg tcttcatcac ctcatcgacg cccgccgact tcgatccgcc
     3781 gagggggccg tgatggcgcg cgtctctggt tgggtgccgg ccgcggtcgc gctcgcggtg
     3841 ctgctgggtg cggtacccac gccgcgcgcg gaatcgatct ttgggatgaa cctactcgga
     3901 gagcgcctgg acgcggtgga cgcgcggacg gcggcgctgg gcgggttcgt gcagaccatc
     3961 gacgattcgc tggggatctt gcaatacaac ccggccatgc tggcgtgggc gaagcgggtc
     4021 acgttcagtg cttccggcta cctgacgcgc gacgccaacc agtccccgga tctcgagcgc
     4081 accgtcgtcg cgtccaagtt ctccgctctc gggctggcgt ttccgctgtt tcgtaagacg
     4141 ctcaccgctt cggtcgggta ccgcgggcgc tacgaccccg acggagactt cagcgtccct
     4201 ggcgaaaccc cggcgggcga gacctacaac gacatcttcg cgcgcagcgg cggcacgtgg
     4261 tcggtgccga tcaccatggc agccgacttg gggcgcttca tgaaggtggg cggcacgttt
     4321 tcgctcgagc gcggcaccat cgagaaccgc tggctcatcg acttcgcggg cacgaccacc
     4381 gcggacgcct cgagcgagca gattcgcgaa gtgcgcggca ctggtttcgc cggcggcgtg
     4441 gtcgtgcgac cgatcgcgag tgtgtcggtg ggcgtcgcct acgaagcgga aatcgactac
     4501 gacgtgaacg taaaggaaag cttcaccaac gccagcgcgg acacgagcta cgaagagacc
     4561 atgcggcagc ccgaacgctg gaccgcgtcc gcgtcgtggc gtgtgatgcg cgggttcacg
     4621 gtctatgcag gtgggtcgct gtgcgacttc accaacttcg agggactcga ctttccgtcg
     4681 gagcgcctga ccgaagagcg cgtggcctcg ttcgggctcg agtatcgctt ccggggttcg
     4741 cgctttccca ttcgcgcgtc ggcccgcttc gagcagctcc cgtacacgat gcccgacggc
     4801 gaggagatct cgcgggtcgc gttcacgctc gggagcggtt tgatctttcg caccggacgc
     4861 ggcaagctcg acgcggcctt gacgtttgcc caagtgggat cggtcgacac caatacctac
     4921 gaggaccagc aggtgcgctt cttcattagc atcgcggggt ccgagagctg ggccaccaag
     4981 cgcgaggggc gctactaatg aggcaccgcg ccgcgaatac ggcgctccgc gcgggcgggg
     5041 tcatcgcgct ggcactcgcg gcggcgtgcg ggcagggcaa gaccgcgggc aagtcgcccg
     5101 cgccggctcc cacgctggcg cagcagctct cgacgatgga gccggaacag cagctcgctt
     5161 tcctgcgagg tctcgagcgc gaaaaacccg gcgacgcgac cgtcgcgttc cacctcggca
     5221 acgcgtatta ttccctcgga agcgcggtgc cggccgaaga gaacgcccgc gcggtggcct
     5281 actacgatag cgctgtcgcg gagtacacgc gggcggtcga gatcgattcg acctactcga
     5341 aggcctacgt caacatgggc ctggcctacg acgccgccac caagcgcccc gacgcgcgcc
     5401 gggtgctgcg caaggcgatc gaggtcaacc ccaacgacgt actggcgtat tgccacctgg
     5461 ggcagatcga gcaaagttac ggggactacg cggaggcggt gcggttgtac cagaaggcgc
     5521 tggtgctcga ccccaacagt gcgcaggcgc actacaacct ggggctcgcg ttcgcggaga
     5581 agaagttgtt caaggaagca ttgatcgagt gggagagagt gatcgtgctc gacccaaatg
     5641 gagagctggg gaagacggcg acggaaaacg tgcggatcat tcgccagtac ctggaggccg
     5701 cgcccaaata atatgaacgg ttcgttcgtc accatgaaag aagcgcgcgc tggcgcgcgc
     5761 gttcttctcg cgatgagcgg cggggtggac tccgccgtct ccgcgctggc cctggtcgaa
     5821 gccggctacc gcgtggtcgg gatgacgatg aagaactact gctatggcga cgcggccgtt
     5881 cccgagcgca gctgctgctc gatggaagcc atcgacgacg cgcgcgcggt gtgccagcgc
     5941 atcgggattc gccacctggt ggtgagcacc gaggagctct tcggccgcga ggtctatcac
     6001 aacttcctgg gcgaataccg ccgcggccgc acccccaacc cgtgcgtgcg ctgcaactcg
     6061 atcgtgcgct tcgacaccct ggtggagtgg gccgaccgca tgggcttcga ccacgtggcc
     6121 accggacatt actcgcgcgt gttccgttcg acgaccgggc gacgttacat cgcgcgcgcg
     6181 aggtctcgtg ccaaggacca gtcgtatttc ctttctgcgc tagacccgcg caccctcgac
     6241 cgcgtgctgt ttccgctcgg cgatcgcgag aaagatgagg tgcgcgctga cgcgcgcgac
     6301 cagggactgc gcgtggccga caagcccgac agccaggaag tgtgtttcgt cggaacgcgc
     6361 acgctgcgcg agttcctgga cggcaaagtc gagatgacgc cgggcgacgt catgacgacc
     6421 gcgggcgacg tggttggccg ccacgaagga ctcgcgacgt acaccatcgg acagcgacgc
     6481 ggcttgggta tcgcggccgc gaagccgaag tacgtggtcg ccctcgaccc cgcgcgcaat
     6541 accgtggtgc tcggcgacga cgaggacctc tatcatagcg aactctcgtg cgacctggcg
     6601 tggatcgacg cggacgccgc gcgagcggcg tctccgctgt ccgcgcagat tcgctcgcgc
     6661 cacagcgctg aacccgtcgc atcgttggcc gtcgagggcg ataccgctcg cgcggtgttt
     6721 catgccccgc agcgcgcgat cgcgcccggc cagaccatcg cgttctacga cggcgacgtc
     6781 gtcgtgggcg cgggcgtcat cgcggcctct ggcccggcgt agggcggcgg ccgtgctccc
     6841 gcgcatccgc gcgatcgcac tcgcacttct tctaacggcc ctggccgcgc cgtcgttcgc
     6901 caacgtccgt tccctcgatc cgtatcccgt cacgattcac taccagccgc gctacagtcg
     6961 tgtcgccgag aaggtcgacg cgatcgcgcg cgcggagctg ccgcgcctga tgtcggaact
     7021 ggggctcgtg caattgagcc cgatcgaaat cgtcgtcacc ggcgacgcgc gcgactacga
     7081 cgcctcgttg acccaccgcc tccccaactg gggggtcgcc ttcgcgatcc tcgacgagca
     7141 gcgcatcctc gtcgacgtcc aaaaggcgac gcgcgcctac aactccctcg acgaagtggt
     7201 cccgcacgag ctctcgcact tactggtacg ccagtgcgtg ccgaacgcgc agctcccgat
     7261 ctggttctcc gagggactgg cgcagtggca ggcgcgcgag tggtcgttgt tcgattcgtg
     7321 gcagctcatg cagggcgtgt ggaccgggag tgtgccgccc atcgaagacc tgggccgtca
     7381 gtacccggcc gacgaagcgc gcgcgcaaga cgcctatcgt atctcgtacg ccgccttcac
     7441 cgacctcttc tccgaagtcg gattcgcgaa gctgccggaa ttcctgggcg cggtcgaagc
     7501 gaaggagagc ttcgcggtcg gcttcgccga ctactggggc ttcaccgtcg ccgactacgc
     7561 ggtctacttc caggacgacg tcgagaagcg ctaccgcagc aaggcgctcg cgttccagag
     7621 cgggcccctt ttcggcttcg cggcgctggt cttcgtggcc gtcatcatcc ggcaatcgat
     7681 caagcggcgg cggaagttcg cgcagctgga ggagtaggga agaggtgcgg cggggccgca
     7741 tcccctttgc ttgaccccct cccaacccgg tgatatagtg ccgattccgg tcgacggacc
     7801 ggagggctag cctaactggt aaggcaacgg tcttgaaaac cgtcgggttt aatcgccctt
     7861 gggggttcga gtccctcgcc ctccgccagt ttgcgggtcg cggtcggcgc ggcccggttc
     7921 gagaagatac ttggagaggt ggccgagtag gtctaaggcg acggtttgct aaaccgttgt
     7981 acgggcttaa acctgtaccg agggttcgaa tccctccctc tccgccatat tcggcgccgc
     8041 ttcgttacgc agcgagcgcc aacgcggagg cgcccccatg ctgatgaagt ccgccgtgat
     8101 caagcgagtc gacctgtggt cgctcttcaa gatggccttc gtgctgtacg cgacgatcgg
     8161 cttcatcggc ggcctcttct acgggatgat cttcgcgttc atcgggagcc tgggcgcggt
     8221 gttgggcgaa gacatgccgg ggctgggggc ggtttcgggt gccatcggca tcattgtcgt
     8281 cccggtcctg gcgatcatga ccggcatcat gggctcggtc gtggtcacca tcatcggcgc
     8341 cgtgtacaac ctcgcggccc gcttcgtcgg gggcgtgaag gtcaccgtcg acgtggccga
     8401 gatcggcccg tcggcgggct acgcaacgac cgcgtcgcct ccggcctcgt aagacatacc
     8461 gcggaaatgg gcgcccgtag ctcaactgga tagagcgtgt ggctacggac cacaaggtta
     8521 ggggttcaag tcctctcggg cgcaccatcc tcgcttttcg cttcgaacga aacctaagga
     8581 aaagctgaaa cttccgccct ccgggcccgg tccaaggagc gtgcacggcg cggccgattc
     8641 tgcaacatcc gtgtgtgcgg gcgcgtagtc ttggccagca cccgacccgg ccgcgggcgg
     8701 tatcttgaaa gacccctgaa agacggagtg acgacgtgaa gaagcccagc cggaaaaccc
     8761 tccttgtttc cctgattgcg gtggtcgtga tcggcgccgg tctcgcgttc gtggccaaga
     8821 cgcgcatggg gatcaagaac agcgcgagcg cggactccac cgtgacgtcg gattccacac
     8881 aagccgccga cgccaccgat aaggacaaga agaaggacaa aaagaagaag aagggtgaga
     8941 aggtcgatgc cccggtcccc gtcgaagtga ccactgtcgc accacgatcg atctccacct
     9001 actaccagac caccgcgacc ctcgagcccg agaagcgcgt cgacattctc tccaagctgg
     9061 ccggcgaggt gcggcagatc ctggtcgaag agggcgactt cgtcaaagag ggtgcgatcc
     9121 tgtgccgcct cgacgacgcc gagctgaagg tcaccctcga ggaggcgcgc atcaaccgcg
     9181 agcagcagaa gctcgagttc gagcgcgtca aggacatgca gacgcagagc ctgatcagcg
     9241 acaaggaata ccagcagatc aagtacaact acgaagtggc ggagaatcga taccagtccg
     9301 cgcgcgtgcg ctacgagtac acgcagatca aagcgccctt cagcggtgtg gtgacgcaac
     9361 gcctggtcga tccgggcgag aacattccca tgggtgcgaa gctcttcatg atgtccgaca
     9421 cccagccgct gcttctctcc atgtacctcc ccgagtccga ggcgcgcctg gtgcgcaagg
     9481 gccagcgcgt ggcgatccga cccgaccagg gcgcggaggc cgagttcgag ggcgaagtgc
     9541 tgcgcatcgc ccccgaagtg gaccagcgca cgggcacggt caaggtgacc gcgcagacgc
     9601 gcgccggcgg cgtgccgggg agcttcgtac gagtgcgcat cctgatggac acgcacgcgg
     9661 acgtgctggc ggttccgcgc cgcagcctgc tttccgacgc gggcgaccgc ttcgtcttca
     9721 tcgccgcggc cgacacggtg cgcaaggtcc aggtcggcat cggctacgag gacgagacgc
     9781 acgcggaagt gaccgaaggc ctggccaagg gcgacaccgt ggtcacggcc ggtgtgggcg
     9841 gcatccgcga cggcaccaag gtcaaggtgg tgcgccccga cgacgttgcc gataaggaca
     9901 aagagaccgc atccaacggc gagaagaagt agcgcgcggc ttcggtccac gcgacaccgc
     9961 catgcgcatc gtcgatttct ccattcagcg acccgtcacc gtgacgatga tcttcgtcgc
    10021 ggtcatcgta ttcggcttcg tagcgctcac gcgcctcgac gtccgtctcc tgcccgagat
    10081 ctcgtatccc tcgctcacca tccaaaccga gtacgaggac gccgcgcccg ccgaagtgga
    10141 gaacttcgtc acgcgccccc tggaagaagc ggtgggcgtc atctccggat tgcgcaccat
    10201 tcgctcggtg tcgaagccgg gcatgtcgga gatcacgctc gagttcacgt ggaagacgca
    10261 catgcagtac gcggccctcg acgtgcgcga caagatcgac ttggtcttgc tcccgcgcga
    10321 gaccaaggcg cccgtgatcc tgcgctacga cccctcgctg gacccggtga tgcgcatcgg
    10381 cgtctcgggc accgagaacc tggtgcgact gcgcaacctc gccgactaca ccatcaagaa
    10441 ggacctggaa tcgctggacg gggtggcgag cgccaaggtg ctgggcggcc tcgaagagga
    10501 gatccaggtc gagatcgacg agcgacgtct cgcgagctac ggcattccca tctcgctcgt
    10561 cgccgcgcgc ctggcccagg acaacgtcaa tcagtcgggc ggtcgcctgc gcgacaaggg
    10621 cgcggagttc ttgctgcgca ccgagaacga gttccagagt gtggaggaca tcgcgaatac
    10681 catcgtgcgc gaagaggcgg ggcgtcgcgt gatcctcgcg gatttgggct cggtaacgcg
    10741 cgggcacgtg gaacgcgacg tcatcacgcg cctcaacggc gaagaagtgg tcgagatcgc
    10801 catctataag gaaggcgacg ccaacacggt ggcggtggcg gacggggtca agcgccggct
    10861 gaatgcgatg aaacgccagc ttccgagcga cgtcacgatc gagaccctgt tcgaccagtc
    10921 gcgcttcatc aagagctcca tcgacgaagt ccgttccaac gcgatggtgg gggcgttact
    10981 cgctatcttg gtgttgtacg tgttcctgcg cgacgtgcgc agcaccgtca tcatcggcct
    11041 ctcgatcccg atctcaatac tgttcacctt tgtcatcatg cagcaactgg gggtggcgct
    11101 gaacatgatg tcgctggggg ggctcgcact cggggtgggc atgctggtcg acaactcgat
    11161 cgtggtcttg gagagcatct cgcgccacaa ggagcgcgag ccggatcgca agctcgcgac
    11221 cctgcggggt gccaaggaag tcgcgggtgc cgtcacgggc tcgacgctca cgaccgtggc
    11281 ggtgttcttg cccatcgtgt tcgtggaagg cattgcgggc caggtgttca aggaccaggc
    11341 gctcacggtg tccatctcgc tcgtggcgtc gctcatcgtg tcgctcatgc tgatcccgat
    11401 agcgtcggca atggacttcg gggcgagcgc gacggcggtg gccgagcccg acgcgcccgt
    11461 ggttccgcgc cggcgcatcg cgcgcgcggc gcggtcgatc gcgttcttct tgaccgtcgg
    11521 cgccccgacc ttcgtgctgc gcacctgccg ccgcgccttg gcctgggtcg ggcgcgggct
    11581 cgcggtcctc gtcaggccgc tccacttggt ctacgaccgt gcctttccgg ccatggagag
    11641 cgcgtatgca cgcgtcctcg agcgcgccct ctcacgccgc cgccgcttcc tgaccggtgt
    11701 attcgcggtg ttcgtcgccg cggtcgcgct ctcgcccctg gtcggacgcg aaatgattcc
    11761 gcagttctcg cagggtgagt tcagcttcgc ggtccagatg ccggaaggaa ccccgctcga
    11821 agcgaccgac gcgcgcatcg tcgacatgga agcaatcgcg gccgcggagc ccggggtcga
    11881 gtcgtacttc accagcgtgg gcaccgccag ccggctcggc agcaacgtga agagcaagga
    11941 cgagaacatc gggcaggtca acgtcatcat gtcggaaaag gggaacaagc acgcggaggc
    12001 ggccctggtc gaatcgctgc gcgccaagtt cgagcgcgtg cagggggtca ccaccaagtt
    12061 cgcgcggccg tcgtacttca acttccagac cccgctcgaa gtgcacgtgt tcgggtacga
    12121 cctggacgag attcgcgact tcgccgaccg gcttgcgctc gacatggccg atattcccgg
    12181 cgtcaaggac gtcaagtcgt cgctcgagta cggcaatccc gagctcgacg tcgaattcga
    12241 ccgcgtccgc atgtcgtcga tgggcctcgc ggtcgaggaa gtggccaaca ccgtgcgcac
    12301 caagatccgc ggcgatgtgc ccacgaagtt caaggagcgc gacaaacagg tcgacatccg
    12361 cgtgcgcacc agcgcgtggc gcgcggaaga cgtcgccgcc atgcgctcgc tggtggtcgc
    12421 ggagcgcgac ggtactccca tcctcctggg cagcatcgcc gacgtgcgag tggcgaccgg
    12481 cgtcaaccag atctcgcgcg tctcgcagca gcgcgccgcc atcgtgtcgg gcaacatatc
    12541 cgggcgagac ctgggtcgcg tcgcgcgcga cgtggaggcg gtgctcgcgt cggcgtcgat
    12601 cccggagggc atcaccgtcg agctgggcgg cgagaacgag gaactgatgc gctcctaccg
    12661 gagcttgggc ttggcgctga ttctggccgt gttcctggtc tacctggtga tggcggcgca
    12721 gttcgagtcg ttcttgcacc ccttcatcat catgttcacg gttccgctgg ccgcgatcgg
    12781 cgtcgtcgcg acattattgc ttaccagcac gccgctgagc gtcatcgtgt tcatcgggat
    12841 catcctgctg gcgggaatcg tggtcaacaa cggcatcgtg ctgatcgact acatcaatac
    12901 actccgccgc gacggcaagt cgaaactgga cgcgattcgg tccgcgggcc gggtccgttt
    12961 tcgccccatt ctcatgacca cgctgactac ggtcttgggg ctgctcccca tggccctcgg
    13021 cctgggcgaa ggcgcggaga tccgcgcgcc catggcattg acggtgatcg gcggtttgtc
    13081 ggtggggacc ctgctcacct tgttggtgat cccgtccctc tacgcggtca tcgcgcgggg
    13141 caagtagcgt gagactccac gagctatcgg tcaggaagcc cgtcctggtg atgatcggac
    13201 tcgtcaccct ggtggcgatg ggcggcatcg cgaccttcaa gctgcccatc gagttcctgc
    13261 cccacgtcga gtttccgttc atcggggtct acattccgta cgccaactcg catcccgact
    13321 acattgagcg ccacatcatc aagccggtcg aagaggtgtt cgccaccctc ggtgacgtgc
    13381 ggcgcatcca atcgcaaacc cagcccgacg gcgccttcat cggcgtcgag ttccagtggg
    13441 gacgagacgt cggcgtgctg cgcatggagg tcaaggagaa gctcgaccag atcaaagggg
    13501 agctcccggc cgacatcgtt cacatgcaga tcttcacctt cgacaccaac gacatcccca
    13561 tcctcgaggg ccgcatctcg gcgggcgggc gcgacctttc ggggagctat gacctgatcg
    13621 agcggtcgat catcaacccc ttgaagcgta tcgagggcgt gggcaacgtg ttcatccacg
    13681 gtgtcgagcc gcgcgagatc gcaatctact tgagcctcga caagatcaag gcccaccgcg
    13741 tcgacgtggg caacgtgttt cgcctgctgc agagcgccaa tgcaaccgcg tcggggggcg
    13801 aaatcaccgc ccacggtgtg cgctactccg tgcgctcgct cggcagcttc aacgacatcg
    13861 acgacgtcga gaatctggtg gtcagccccg agggcctgcg cctgagggac atcgccgata
    13921 tctactacgg agagcccgcc gtcgcgtacg gccggcggct caacggcgag gccgcggtcg
    13981 ccttctggat ccaaaaagcc tccaacgcca acacggtcga cgtcgcgcgc cgcgtgcacg
    14041 cggccatgga gaagatgaac gacgaccccg cgctcgaagg gatcgatgtg ctcctgttct
    14101 tcgaccagtc gaaggagatc ctcaacggcg tcagcggact gcgcaacgcc ggtttccagg
    14161 gcggcctgtt ggccatcggc attctgtatt tcttcctgcg gcgcgtcacg accacgctga
    14221 tcatcgccat cgcgattcca ttctcgctgg tgtgcacgct cgcgttcctc tatttcacgg
    14281 gctacacgct caacatgctg accatgatgg ggctcatgct gggggtgggg atgctggtcg
    14341 acaacgccat cgtggtgctg gaatcgatct atcagcacca actgcgtcgc gaggacccga
    14401 tgcacgccgc cgtcaccggc gccgactcgg tggccgtggc cgtcaccgcc tcgaccctga
    14461 cgtcggtgat cgtgttcgcg cccatcgtgt tcgggtccaa gaacgacgaa ctgttcgtct
    14521 ggctctcgtc ggtcggggtg accatctcgg tcgcgatcct gttctcgctc ttgctctcgc
    14581 tgaccatgat cccgtttctc gcctcacgct tgaagcgccc caaggacgtg cgcccgtcac
    14641 ggttcttgaa gtcagtcgag aaccactacg tgcgcgcgct ggaatgggcc acgttcaagc
    14701 accgcaagtg gacgctggga atcgtcgccg gttcgctgct gttgaccgtc gtggccgtca
    14761 aggtgatcgg cttgaagggc ttccaggacg acgactcctt catcatcgag cgcatattcg
    14821 ttgaatacga ctttgcggag aacgtggact acaaggaaac caaccagttc gtgcggcgcg
    14881 tcgaagccgc cctggaggcc aagcgggatt cgttgggcat cgagaccatc tacagctatt
    14941 acgaagacaa ccgcgccatg acgacggtgt acttcgagca ggaacgcttg agccgcgaca
    15001 tcctcaagga gaagcgcaag gccctgcgcg ccgccatccc cgagatggcc ggtgtcaagc
    15061 tgcgcttcgg ggacgagagc ggtcaggaca gcggcggcgc gtcgctcatg caggtcaacc
    15121 tgttcggcga ggacaagcag gtgctggagg agatcgcgaa cgaggtcaag cgccgcttcg
    15181 gctacctgga ggatctcacc gacgtcaaga ccagcgtcga gctggggcag gaagaaatca
    15241 gcgtggccgt cgacgccgac ctgctcgcgc gagcgcaaat gacggccaac gacgtggttc
    15301 aggtgatggg gctgacgttc cgcggcttcg agttgaatcg cttccgcggc gaggatcgcg
    15361 aagtgccgat gtcgatcagc ctcgacccca ccgaccaggt cgggatctac aatcttcaca
    15421 atctaatggt ggggatgaac gacgaccgcg aggtgaccct cggctcggtg gccgagttcg
    15481 agcagaagcg cgggcccacc cagatcgagc gcgacaacca gcgcaccatc gtgtcggtac
    15541 gcggactcta cgagggcgaa cgcttcgacg agctgaagga ggaagtgggc gccgtcatgc
    15601 gctcgctggc gctgccgccg ggctacgcgt ggtccttcgg tcgccgcatc gaggagcgcg
    15661 acgcccaggc ctcgcaaatg ctggtcaacg cgctgctcgc gattctatgc gtctacctga
    15721 tgatggcggc cctgttcgag tcgtacttgc atccgctggt gatcatgatc tgcttgccgc
    15781 tggcgggcat cggcgtggtc tggatgctga tgctgacggg gaccgcgttc agcctcatgg
    15841 ccatgatcgg caccgtcatc ctgatcgggg tggtggtcaa caacggcatc gtgctgatcg
    15901 accacgtcaa tcatctgcgc aaggaaggca tgccgatgga gcaggccatc ctggaaggcg
    15961 ggcgcgagcg cctgcgaccc attctcatga ccgcgctcac caccatcctc ggcctcagtc
    16021 ccatggccat cggcggttca cacatcggcg aggcgcagta ctacccgctc
//
LOCUS       DASPGK010000321         8257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_782487, whole genome shotgun sequence.
ACCESSION   DASPGK010000321 DASPGK010000000
VERSION     DASPGK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782487"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /gene="gpmI"
                     /locus_tag="VEC56_09110"
     CDS             <1..1291
                     /gene="gpmI"
                     /locus_tag="VEC56_09110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXV14349.1"
                     /translation="RAIRDGSFFANPALVEAMDRVRARGALHLIGLLSDGGVHSHQQH
                     LYALLKMAKDRGVKDVFVHAILDGRDTAPHGGERYLRELEERMRAEGVGSIATVVGRY
                     YAMDRDKRWERTRRGYDLFTLGEGAAAASAAEAIGRSYDAGITDEFVEPVRVGSGPRG
                     LVGDGDSVVFFNFRADRARQITHAFTDEPFAGFERRARPRVHYVGMARYEDTLDLPSA
                     FAPESRRDILVDTASANGKATLRIAETEKYAHVTYFFNGGEEHEYAGETRVLIPSPKV
                     ATYDLKPEMSAAEVTDALIREIRSDRHDYIICNFANPDMVGHTGVLAAAIKACETVDA
                     CVGRVMDAIDLARYAVIVTADHGNCELMIDPATGGPHTAHTTNPVPCILVDPDYHGAL
                     IEGGSLRDIAPTILDYLGVARPSGMTGRDLRATRPAG"
     gene            complement(1309..2466)
                     /locus_tag="VEC56_09115"
     CDS             complement(1309..2466)
                     /locus_tag="VEC56_09115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV14350.1"
                     /translation="MTPDRRTLLALFALAFGLRILYAVILGDPIAGALDSYGYRIASR
                     MVESWEWISTPFSPSAPGYRMLLAAAFKIFGVSWWTAVLLNAVLGAVTTLFVYRIGER
                     LLGKRVGLVSALWLGLSVGQMYFASLVTRDVLVVFLLTWLAYALARPFYRMRTATWTA
                     FLCTLLLHSEPMFLVLLPLLVVFLALKSTHHRALSAQYVFLFLAALFVFFLPWTMRNW
                     FVYRDVVPVGLEATRYTAPVARLLRDLPPEPSAAAHPGATTFEKPGFFQNQREFWRVV
                     RLTDAPADPARGRPTEPAWSLRHNAASALSYGLLLPFFLVGLVYAVARRHRAALVLGS
                     IVVAYALLRGFLWTNDYSRLWVEPLIILVAFYGVRVLLDLRRAAGEPDAST"
     gene            complement(2571..2948)
                     /locus_tag="VEC56_09120"
     CDS             complement(2571..2948)
                     /locus_tag="VEC56_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HXV14351.1"
                     /translation="MSCAGALVALAMVAGCGGDGGPSGGNGGPTGPRTHAVTVDDGSF
                     YPEQITIAPGDSVLWTWAVNDDHSVTSGTSPDPGEDPRMFDSGIQSSGSFGHRFAQIG
                     SFSYFCREHWDMGMNGRVTVEQP"
     gene            complement(3033..3446)
                     /locus_tag="VEC56_09125"
     CDS             complement(3033..3446)
                     /locus_tag="VEC56_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14352.1"
                     /translation="MTDFAKTVRTHFDFLASDFGFVPTSEDAGGVRYDSATLCVEIGA
                     GKGEIDLVFRVKVDTDVLRPYISHVFSLAEVVRYYKTGPFPRFDSFPPIPGASEEQRY
                     VIYLAALTRNYCEEILRGDITALERLSVNRGAKNT"
     gene            complement(3469..4212)
                     /locus_tag="VEC56_09130"
     CDS             complement(3469..4212)
                     /locus_tag="VEC56_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV14353.1"
                     /translation="MTAEFSNVYDDGARADAYAKLEFPGTYYLAFRDLPAIIGRYVRG
                     RKALDFGCGAGRSTRFLRELGFDVEGVDISEPMLARARACDPAGRYSLVPDGDLGGLP
                     ARAYDLVLSAFTFDNIPTMERKIALLTSLQRLLKDGGRIVNLVSAPDIYLHEWASFST
                     KDFPTNRSAQSGDKVLIVMLDVEDRRPVVDILCTDADYQDAYRRAGLRPIATYRPLAN
                     PTEAFSWVSETTIAPWVIYVLGAAADHSS"
     gene            4353..5453
                     /locus_tag="VEC56_09135"
     CDS             4353..5453
                     /locus_tag="VEC56_09135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV14354.1"
                     /translation="MAVPFLDLKAQYASIKGEIDAAIMGVVESCGFIGGETLRRFERN
                     FAAYSGAKFGVGSCSGTSALHLALLGAGVKRGDEVITACNTFIATTEAIMHAGATPVL
                     VDVDEATQLIDPARVEAAVTSKTRAIIPVHLYGQPADMDAINDIAKRRGLKVIADAAQ
                     SHGSAIGGDRKKTLGHTTAFSFYPGKNLGAYGDAGLVATDDEDVATYMRALGDHGSKE
                     KYHHLYEGWNYRLDAIQAAVLDVKLKHLDQWTERRRAHAARYDQAFAGTAVTPTTAVP
                     GRTHVYHLYVVQVRDRDATMARLKEKGIGTGIHYPLPLHLQPAYAHLGLKKGAFPTAE
                     KVVARIVSLPMYAEMTDAMVDEVAAATIAAAG"
     gene            5515..6624
                     /locus_tag="VEC56_09140"
     CDS             5515..6624
                     /locus_tag="VEC56_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14355.1"
                     /translation="MRSAAVLLLLWVALGFTPIRAATDSRPILYLALEGAGESRHVMV
                     RAVSQDGSQVDFSIAGTAPAWSPDGRWIGYVSEDGQLFVTNTSNESKKLLRVDEGGEL
                     LIAHPMWSPDGGRIAVVVRALEANNKITSALVIVDVATRKVRTSHKLPDDVVAFPAHL
                     TPPGRFRWSPDGRHVLVAWEYAVVVDVEKGRVETISKDPIVGEWVGDHIYYIAVPRAK
                     DSVNRVYNGFYAKRPGQAATELMNQAELEAAGVGVAAPTYGSMVAAPNGAALAVSTGS
                     PDVGSSVLRVCDLKAGAPSLADPAQKFELDGGVVALDWAPGQDSLAAVIVAGNVVSVV
                     RIDVTSGERQVLATWQFEDPMEVELFVHKALSWTR"
     gene            complement(6657..>8257)
                     /locus_tag="VEC56_09145"
     CDS             complement(6657..>8257)
                     /locus_tag="VEC56_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14356.1"
                     /translation="VRALQYAAGPENFDRILHEYFDRWKFKHVNEERFRLVCEEVAQR
                     DLSRQFEQWLHTKKTVDYALESVRTRPDSTGATTEVVIKRKGELYLPIEVHFTLPDGR
                     VEKKRFDARDREFRERVKLPAVPVKVDINPDNEIMDIDRSNNVYPKQRDFQIDWPNNF
                     YYPEDAYQIRHRPAIWYNDIDQAKVGYHLSGSLHGWKRRVNLGVYYGTKSEQWDFSAS
                     YEEPKRWLNTNTTLRLSGYRMEGRADARIEVAFKKRGKLIEPPTQEITFGYGYHELRD
                     AAYLTSSEIYDTSLADVGPYVSYAVDPQLDIAATRFAVDFTMGRDWFAGNTDYERIAM
                     LAAFHSRPEVVRLDTRLRFFGGFGGSLPTQRKFNLAGGGPMKQEERFWLRAPGAVPED
                     LNYVEPGDGNLRGYAAGTFGVNKLVAMNAEMGTKLKLFGLEKPLAKLIGTPSWYGFYD
                     VGWILDGDNPIQSSARVTSLVDQGVLDATLENAGVGFRGNVTWPFWNFSWRLDIPFWV
                     SHPRINGEADYVDFRYLFSLNASF"
BASE COUNT         1519 a         2675 c         2706 g         1357 t
ORIGIN      
        1 ccgcgccatc cgcgacgggt cgttcttcgc gaatccggcg ctggtcgaag ccatggaccg
       61 cgtacgcgcg cgcggcgcac tccatttgat cggactcttg tccgacggcg gggtgcacag
      121 ccaccagcaa cacctctacg cgctcttgaa gatggcgaag gatcgcggcg tgaaggacgt
      181 cttcgtgcac gcgatcctcg acggccgcga caccgcaccg cacgggggcg agcgctacct
      241 gcgcgagctc gaggagcgca tgcgcgcgga aggcgtgggc tccatcgcca ccgtggtggg
      301 acgctactat gcgatggatc gggacaagcg ctgggaacgc acgcggcgcg ggtacgactt
      361 gttcacgctg ggcgaaggcg cggcggccgc gagcgcggcc gaagcgattg gccgctcgta
      421 cgacgccggc atcaccgacg agttcgtcga gccggtgcgc gtgggatcgg ggccgcgcgg
      481 gctggttggc gacggcgact cggtggtgtt ttttaatttc cgcgccgacc gtgcgcgtca
      541 gatcacgcac gccttcaccg acgaaccctt cgccggcttc gagcggcgcg cgcgtccccg
      601 cgtgcactac gtcggcatgg cgcgctacga agacacgctc gaccttccga gcgcgtttgc
      661 tcccgaatca cgccgcgaca tcctggtcga caccgcgtcc gcgaacggca aggccacgct
      721 gcgcatcgcc gagaccgaga agtacgcgca cgtcacctac ttcttcaacg gcggcgagga
      781 gcacgagtac gcgggcgaga cgcgcgtgct gatcccctca cccaaggtcg cgacctacga
      841 cttgaagccg gagatgagcg cggccgaagt gaccgacgcc ttgatccgcg agatccgctc
      901 cgatcgccac gactacatca tctgcaactt cgcgaatccg gacatggtgg ggcacacggg
      961 cgtgctggcg gccgcgatca aggcctgcga gaccgtggac gcgtgcgtcg ggcgcgtgat
     1021 ggacgcgatc gacctcgccc gctacgcggt catcgtcacc gccgaccacg gcaactgcga
     1081 attgatgatc gaccccgcca ccggcggccc gcacacggcg cacacgacca acccggtgcc
     1141 gtgcatcctg gtcgaccccg actatcacgg cgcgctcatc gaaggcggct ctttgcgcga
     1201 catcgcgccc acgattctcg actacctggg agtcgcgcgg ccaagcggga tgacggggcg
     1261 cgatctgcga gcgacacgac ccgccgggta gcgttcgagt gagacggcct acgtcgacgc
     1321 gtccggctcg ccggccgcgc gccgcaggtc gagcaagaca cgcaccccgt agaacgccac
     1381 caagatgatc agcggttcga cccagagccg cgagtagtcg ttcgtccaca ggaatccccg
     1441 cagcagcgca tacgcgacca cgatcgagcc aagcacgagt gccgcgcgat ggcggcgagc
     1501 caccgcgtac acgagcccca cgaggaagaa cgggagcagg aggccataac tcagcgcgct
     1561 ggccgcgttg tgtcgcagcg accacgcggg ttcggtcggg cgaccgcgcg cggggtcggc
     1621 gggggcgtcg gtgagacgca cgacgcgcca gaactcgcgc tggttctgga agaagcccgg
     1681 cttctcgaag gtggtcgcgc cggggtgcgc ggctgccgaa ggctcgggcg gaagatcgcg
     1741 cagcaggcgt gcgaccggcg cggtgtagcg ggtcgcctcg agcccgacgg gtaccacatc
     1801 gcggtagacg aaccagttgc gcatcgtcca gggcagaaaa aacacgaaca aagcggccag
     1861 gaacaggaac acgtactgcg cgctcaaggc gcggtggtgg gtggacttga gggcgagaaa
     1921 gaccaccaac agtgggagca ggaccaggaa catcggctcg ctgtggagca ggagcgtgca
     1981 caagaacgcg gtccaggtcg cggtgcgcat gcgatagaac ggccgcgcga gcgcgtaggc
     2041 gagccacgtc aagaggaaga ccacgagcac atcacgcgtc accaagctcg cgaaatacat
     2101 ctgcccgacc gataaaccga gccacaacgc ggataccagt cccacgcgtt tgccgagcag
     2161 gcgctcgccg atccggtaca cgaacaaggt ggtgaccgca cccaggacgg cgttgagcaa
     2221 gaccgccgtc caccacgaga cgccgaagat cttgaacgcg gccgccagga gcatgcggta
     2281 cccgggggcg ctcggcgaga agggggtcga gatccattcc cacgactcga ccatgcgcga
     2341 cgcgattcga tagccgtagg aatcgagcgc tcccgcgatg gggtcgccga ggatgacggc
     2401 gtagagaatg cggaggccga acgctagcgc aaacagcgcc agcagcgttc tacggtcagg
     2461 ggtcaacgct acctcacggg cccggagccg ggggccagat cgagtgagtt cggatgtcgg
     2521 tgtgagtcta cgggccgcgt ttcgtgcgtg tcaatcgaac gaatcggaac tcaaggttgt
     2581 tcgaccgtca cccggccgtt catccccatg tcccagtgct cgcggcagaa gtacgagaag
     2641 gacccgattt gcgcgaagcg atgaccgaaa ctccccgacg attgtatacc cgaatcgaac
     2701 atgcgcgggt cctcgccggg gtcgggcgag gtccccgaag tcaccgagtg gtcgtcgttg
     2761 acggcccacg tccacaggac gctgtcgccg ggcgcgatcg tgatctgttc cgggtagaag
     2821 gaaccatcgt ccaccgtgac cgcgtgcgtg cgcggccccg tcggaccgcc gttgccgccg
     2881 ctcggcccac cgtcgccgcc gcagcccgcg accatcgcca gcgcgacgag tgcgcccgcg
     2941 caggacatcg ctcgtgacca acgggaagcc atcgcgcgga atcgtgcaat tgccgtgccc
     3001 gggaagggcc gcgaacgacg gcgccgtcgt ggctaggtat tcttggcgcc ccgattcacc
     3061 gaaagccttt ccagtgccgt gatgtctccc cggaggattt cctcgcagta gttcctcgtg
     3121 agcgcggcca aatagatcac atagcgttgt tcttcgctgg ccccgggaat gggcggaaac
     3181 gagtcgaagc gcgggaacgg cccggtcttg tagtagcgaa ccacctcggc gaggctgaac
     3241 acgtgcgata tgtacggtcg caggacatcg gtgtcgacct tgactcgaaa gacaagatcg
     3301 atctctccct tgccggcacc gatctccacg caaagcgtgg ccgagtcgta gcggacaccg
     3361 ccggcgtctt cggacgtcgg aacgaatccg aaatcgctgg ccagaaagtc aaaatgggtt
     3421 cgaacggttt tcgcgaagtc cgtcatggtt ccagcggtcc caatagtgtc aactcgaatg
     3481 gtcggcggca gctcccagca cgtagatcac ccaaggtgcg atcgtcgttt cgctcaccca
     3541 cgagaacgct tcggtcggat tcgcgagcgg cctgtaggtt gcgatcggtc gcagccccgc
     3601 gcgccggtac gcgtcctgat agtcggcatc cgtgcacagg atgtccacga ccgggcgacg
     3661 gtcctcgacg tcgagcatga cgatcaagac cttgtctccg ctttgcgcgg agcggttggt
     3721 cggaaagtcc ttggtggaga acgacgccca ttcgtgcagg tagatgtcgg gggccgatac
     3781 cagattcacg atacgcccgc cgtccttgag caaccgttgc agggaggtca agagcgcgat
     3841 ctttcgctcc atcgtgggaa tattgtcgaa ggtgaacgcg gacaatacga ggtcgtacgc
     3901 tcgcgccgga agcccgccca ggtcgccgtc cggcacgagc gaataccgtc ccgccgggtc
     3961 gcacgcgcga gcccgcgcta gcatgggctc cgagatgtcc acgccctcga cgtcgaagcc
     4021 cagttcccgc aggaagcgcg tcgaacggcc ggccccacac ccgaaatcga gcgccttgcg
     4081 gccgcgcacg taccggccga tgatcgcggg tagatcgcga aacgccaggt agtacgtgcc
     4141 cggaaactcg agtttcgcgt aggcgtcggc gcgcgcgcca tcgtcgtaca cgttggagaa
     4201 ttctgcggtc atcttccagc ctcgggagtg ctcgatggca agtgtaatgc gtgctccgcg
     4261 accccacaag tcgcttgctc gcggcccgtc ggtatgctaa cctccgccgc ttcaacctca
     4321 gacccgcaca ccagaattcg gagttttaca ccatggctgt tccgttcctc gatctcaagg
     4381 cacagtacgc atccatcaag ggcgagatcg acgccgccat catgggcgtc gtcgaaagtt
     4441 gcgggttcat cggcggcgaa acgctccgcc gcttcgagcg caacttcgcc gcctacagcg
     4501 gcgccaagtt cggcgtcggg agctgcagcg gcacctccgc gctgcacctg gcactgctcg
     4561 gcgccggagt caagcgcggc gacgaagtga tcaccgcgtg caacaccttc atcgccacca
     4621 cggaagccat catgcacgcg ggtgcgacac cggtcctggt cgacgtcgac gaagccaccc
     4681 agctcatcga tcccgcgcgc gtagaagcgg ccgtcacctc caagacgcgc gcgatcattc
     4741 cggttcacct ctacggccag cccgccgaca tggacgccat caacgacatc gcgaagcgcc
     4801 gcgggttgaa ggtcatcgcc gacgccgcgc agtcgcacgg ctccgcgatc ggcggcgatc
     4861 gcaaaaagac gcttgggcac acgaccgcgt ttagcttcta ccccggaaag aacctgggcg
     4921 cgtacggcga cgcggggttg gtggccaccg acgacgaaga cgtcgccacc tacatgcggg
     4981 cactcggcga ccacgggagc aaggagaagt accatcatct ctacgaaggc tggaactatc
     5041 gcctcgacgc catccaggcc gcggtcctcg acgtcaagtt gaagcacctc gaccaatgga
     5101 ccgagcgtcg acgcgcgcac gcggcgcgct acgaccaggc cttcgcgggc accgcggtca
     5161 cgcccaccac agccgtgccc ggccgcacgc acgtctatca tctatatgtc gtgcaggtgc
     5221 gcgaccgcga cgccaccatg gcgcgcctga aggagaaggg gatcgggacc ggcatccact
     5281 acccgctgcc gctgcacctg cagccggcct acgcgcactt gggactgaaa aagggtgcct
     5341 tcccgaccgc ggagaaggtg gtggcgcgca tcgtgtcgct tccgatgtac gccgagatga
     5401 cggacgcgat ggtggacgaa gtcgccgcgg cgacgatcgc ggcggcgggg tagcggttcc
     5461 gcgttgtgcg gcgtgcgagt cgaggcatcg cacgacgagg ccaaggaggc ccacatgaga
     5521 agtgctgctg tcttgttgtt gctctgggtt gctctgggtt tcactccgat tcgcgccgca
     5581 acggactcac gaccaatcct ctatctcgcg cttgaaggcg cgggagaatc tcgccacgtc
     5641 atggtgaggg ccgtctcgca agacggtagt caggttgatt tctccattgc cgggacggca
     5701 cctgcctggt cgcccgatgg ccgttggatt gggtacgtga gcgaagacgg ccagttgttc
     5761 gtgaccaaca cgtccaacga gagcaagaag ctcctccgcg tcgacgaggg aggtgaactg
     5821 ctgatcgccc atcccatgtg gtcacccgac ggaggtcgca tcgctgtggt cgtgcgcgcg
     5881 ttggaagcga acaataagat caccagtgcg ctcgttatcg tcgacgtagc cacgcgcaag
     5941 gtccgcacga gccacaagct acccgacgac gtcgtcgcgt ttccggcgca tttgacgccg
     6001 cccggaaggt tccggtggtc gcccgacggc cggcacgttc tggtcgcgtg ggagtatgcg
     6061 gtcgttgtcg atgtcgagaa gggacgggtc gagacgattt ccaaagaccc aatcgtcggc
     6121 gaatgggtag gcgatcacat ttactacatc gccgtgccgc gcgcgaagga cagcgtgaat
     6181 cgcgtataca atgggtttta cgcgaagcgc cccggtcaag cggccacgga gttgatgaat
     6241 caggccgagc tcgaagcggc gggtgtgggc gtcgcggccc cgacctacgg atcgatggtc
     6301 gcggcaccga acggcgccgc gttggccgta tccaccgggt cgcccgacgt tgggagcagc
     6361 gtccttcgcg tgtgcgacct caaggccgga gctccatccc tcgcggatcc ggcgcagaaa
     6421 ttcgaactcg atggtggcgt ggttgcgctc gactgggcgc cgggccaaga ttcattggcg
     6481 gccgtcattg tcgctggtaa cgtcgtcagc gtcgtacgta tcgacgtgac gtccggcgag
     6541 cggcaggtgc tagccacgtg gcaattcgag gatccaatgg aagtcgagct gttcgtccat
     6601 aaggcgttga gctggacccg ttgatcccgc tctcgcacct gccaggccga tagcgcctag
     6661 aacgacgcgt tcaagctgaa cagatagcgg aagtcgacgt aatccgcttc gccgttgatc
     6721 ctcgggtggc tcacccagaa ggggatgtcg aggcgccaag agaagttcca gaagggccac
     6781 gtcacgttcc cgcgaaatcc cacgcccgca ttctctagcg tcgcatccag cacgccctgg
     6841 tccaccagcg acgtcacgcg tgcgctcgat tgaatcgggt tgtcgccgtc gaggatccac
     6901 cccacgtcgt agaagccgta ccacgacggc gtcccgatta gcttcgcgag cggcttctcc
     6961 agcccgaaca gcttgagctt ggtgcccatc tcggcattca tcgccaccag cttgttgacg
     7021 ccgaaggtgc cggccgcgta gccgcgcagg ttgccgtcgc cgggctcgac gtagttcagg
     7081 tcctcgggca ccgcgcccgg tgcgcgcagc cagaagcgtt cctcttgctt catgggtccg
     7141 ccgcccgcca ggttgaactt gcgctgcgtc gggaggcttc ctccgaagcc gccgaagaac
     7201 cgcaggcgcg tgtcgagccg cactacctcc ggacgcgaat ggaacgccgc cagcatcgcg
     7261 atgcgttcgt agtcggtgtt gcccgcgaac cagtcgcggc ccatggtgaa gtccacggcg
     7321 aagcgcgttg ccgcgatgtc gagctggggg tcgaccgcgt acgatacgta cggcccgacg
     7381 tcggctagcg aggtgtcgta gatttcgcta ctcgtgaggt acgcggcgtc gcgcagctcg
     7441 tggtagccgt acccgaacgt gatctcctgc gtaggcggct cgatcagctt gccgcgcttc
     7501 ttgaacgcca cctcgatgcg cgcgtcggcg cggccctcca tgcggtagcc ggacaaacgc
     7561 agcgtcgtgt tggtgttgag ccagcgcttg ggctcttcgt aggacgcgga gaagtcccac
     7621 tgctcgctct tggtcccgta gtacacaccc aggttcacgc gccgcttcca cccgtgcaag
     7681 ctgccggaca ggtggtagcc caccttggct tggtcgatgt cgttgtacca aatggcgggg
     7741 cgatgacgaa tctggtacgc gtcctcggga tagtagaagt tgttgggcca gtcgatctga
     7801 aagtcgcgct gcttcgggta cacgttgttc gaccggtcga tgtccatgat ctcgttgtcg
     7861 ggattgatat cgaccttcac cggaacggcc ggaagcttca cgcgctcgcg aaactcgcgg
     7921 tcgcgcgcgt cgaagcgctt cttctccaca cggccgtcgg gtagcgtgaa gtggacctcg
     7981 atggggaggt acaactctcc tttgcgcttg atgacgacct cggtggtggc gcccgtcgag
     8041 tccgggcgcg tgcgcacgct ctcgagcgcg tagtcgaccg tcttcttggt atggagccac
     8101 tgctcgaact ggcgcgagag gtcgcgctgg gccacttctt cgcacaccag ccggaatctc
     8161 tcctcgttta cgtgcttgaa cttccagcgg tcgaagtact cgtgcaggat gcgatcgaag
     8221 ttctcggggc cggcggcgta ctggagcgcg cgcacca
//
LOCUS       DASPGK010000322         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1133621, whole genome shotgun sequence.
ACCESSION   DASPGK010000322 DASPGK010000000
VERSION     DASPGK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133621"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VEC56_09150"
     CDS             <1..738
                     /locus_tag="VEC56_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV14357.1"
                     /translation="GLPDKADLCPDLAEDSDAFEDGDGCPELDNDQDGIQDAQDKCPD
                     EPEDFDGEADDDGCPDLVKDSDNDGVSDDLDRCPLQAEDVDGYQDDDGCPDLDNDLDG
                     IPDATDKCPTQPETFNNVDDTDGCPDAKEIGKQFILRGINFESGSAALTPDSHPVLDQ
                     VIASMNQYPEVRIEIRGHTDSQGPAAFNLELSQKRAQSVRQYLINGGIAGDRITAIGV
                     GEEEPISSNGTAEGRAQNRRIEFKRLN"
     gene            complement(815..1609)
                     /gene="mazG"
                     /locus_tag="VEC56_09155"
     CDS             complement(815..1609)
                     /gene="mazG"
                     /locus_tag="VEC56_09155"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXV14358.1"
                     /translation="MTASNTDDGRALARVFELIARLRAPDGCPWDRAQRLEDVLSDLI
                     EEAYELEWAGTRRGSAELLDEFGDVVFLVCFAIAIQRETDPSFTLAALADYAHDKIKS
                     RHPHVFGDAKAATADESIVHWERIKSEEREKKGAGVLDGVAGNLPPLRHAVKIQERAG
                     AVGFDWDDAAGTIDKLREEVAELEAALHAGDRAHVEDEMGDVLFAAANVGRFLKVDTD
                     DVLRKSTVKFIRRFQLMEAMIRDDGRRFQDMTLTEMNAYWERAKAA"
     gene            1726..>3802
                     /gene="uvrA"
                     /locus_tag="VEC56_09160"
     CDS             1726..>3802
                     /gene="uvrA"
                     /locus_tag="VEC56_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXV14359.1"
                     /translation="MIDKIVVRGAREHNLKNIDVEIPRGSLTIITGVSGSGKSSLAFD
                     TLYAEGQRRYIESLSTYAKQFLERIGRPDVDEVTGLSPAVAIEQRNTTRSSRSTVGTA
                     TEIYDYLRLLFARVGRTVCPKCHLEVRRWAPDEVIDALLGDMPGRAVFLVATRAVEKG
                     SGVKLFEELIREGYTRYRVAGDVVRLEAAPKGAARMKSIDVVLDRVEIDESRRARLLE
                     AIETAYRMADGVVRVDDAETGEARSFTNTLACTSCGRTFEEPRPILFSFNTPFGACPQ
                     CRGFGSRMEFDPALVVPDPTKSIREHAVEPWASEKFEYFYDQLVRFCRRKKIPMDKPW
                     SGLKEAERRAIMDGEGEYVGVMPFLENMRQKTYKKYARFFTRRYLAFRECRACGGGRL
                     REEAFFVRLGGRTIRDVAAMSPGEALAFVRGLSFNERERTVAKDVLIELESRLEFLLA
                     VGLHYLTLDRLAKTLSGGESQRISLANSLGSSLLDVLYVLDEPSVGLHPRDTDRLVRV
                     LGDLRARGNTVVVVEHDLDIIRAADHVVDLGPGAGEGGGRVVYQGPPADATTGTLRFL
                     VEGPPIVTRAGARAPEAFMVLRGVREHNLRDADVALPLGSFTSVTGVSGSGKSTLVCD
                     VLYDALRAKNGHGGAAYRALEGAGAVRGVLMVDQAPIGKTPRSNPVTYIKAFALVREI
                     FAAQKKAARR"
BASE COUNT          637 a         1268 c         1296 g          601 t
ORIGIN      
        1 gggctgcccg acaaggccga cctgtgcccc gatctggccg aggattcgga cgcgttcgaa
       61 gacggcgacg gctgccccga gctggacaac gaccaggacg gcatccagga cgcgcaggac
      121 aagtgcccgg acgagccgga agacttcgac ggtgaggccg acgacgacgg ctgcccggac
      181 ctggtcaagg acagcgacaa cgacggcgtg tccgacgacc tcgatcgctg cccgctgcag
      241 gccgaggacg tcgacggcta ccaggacgac gacggttgcc ccgatctcga caacgacctg
      301 gacggcattc ccgacgcgac ggacaagtgc ccgacacagc cggagacgtt caataatgtg
      361 gacgataccg acggctgccc ggatgcgaag gagatcggca agcagttcat cctgcgcggg
      421 atcaacttcg agagcgggag cgcggcgcta acgcccgact cgcacccggt cttggaccag
      481 gtgatcgcgt ccatgaacca gtatcccgag gttcgtatcg agattcgcgg ccacaccgac
      541 agccagggcc cggccgcgtt caacctcgag ctgtcgcaga agcgcgccca gtcggtgcgc
      601 cagtacttga tcaacggcgg catcgcgggt gaccgcatca cggccatcgg agtcggcgaa
      661 gaagagccga tctcgtccaa cggcacggct gagggacgcg cgcagaaccg ccgcatcgag
      721 ttcaagcggc taaactaatc acgcacgcaa cgcgtgaaac gaagaagggc cggtcgcaag
      781 accggccctt tttttcgtgc gagcctcgag tcgctcacgc cgccttcgcg cgctcccagt
      841 aggcgttcat ctcggtgagc gtcatgtcct ggaagcggcg gccgtcgtcg cggatcatcg
      901 cttccatcaa ttgaaagcgc cggatgaact tcacggtgga tttgcgcagg acgtcgtcgg
      961 tgtcgacttt gagaaagcga cccacgttgg cggccgcgaa aaggacgtcg cccatctcgt
     1021 cttcgacgtg ggcgcggtcg cccgcgtgga gggcggcttc cagctccgcg acctcttcgc
     1081 gcagcttgtc gatggtcccg gcggcgtcgt cccagtcgaa gccgaccgcg cccgcgcgct
     1141 cctggatctt gaccgcgtga cgcaggggcg ggaggttgcc ggcgactccg tcgagcacgc
     1201 ccgcgccctt cttctcgcgc tcctcggact tgatgcgctc ccagtgcacg atgctctcgt
     1261 cggcggtggc ggctttggcg tcgccgaaca cgtgggggtg gcgcgatttg atcttgtcgt
     1321 gggcataatc cgcgagcgca gcgagcgtga atgaggggtc ggtctcgcgc tgaatcgcga
     1381 tcgcgaaaca aaccaggaac acgacgtccc cgaattcatc gagtagttca gcacttccgc
     1441 gccgcgttcc cgcccactcc agctcgtagg cctcttcgat gaggtccgac aagacgtcct
     1501 cgaggcgctg cgcgcggtcc cacgggcagc cgtcgggtgc gcgcagccgc gcgatcagct
     1561 cgaacacgcg cgccagcgcg cgtccgtcgt cggtgttgga agccgtcatg tcggccatca
     1621 taaagtcgcc gcgatcgcgc aaacaaggca aacttggtcc ttgccggcgc gtccggaagg
     1681 tgcctacaat cagcggtttc atccggcccg aagggccggt tccctatgat cgacaaaatc
     1741 gtcgtgcgcg gtgcgcgcga gcacaacctc aagaacatcg acgtcgagat tccgcgcggc
     1801 agtctcacca tcatcacggg tgtgtcgggc tcggggaagt cctcgctggc cttcgacacc
     1861 ctctatgctg agggacagcg ccgctacatc gagtcgctct cgacctacgc caagcagttc
     1921 ctcgagcgca tcgggcgccc cgacgtcgac gaggtgacgg ggctttcgcc cgcggtggcc
     1981 atcgagcagc gcaacaccac gcgcagctcg cgctcgaccg tcggcacggc caccgagatc
     2041 tacgactacc tgcgcctgtt gttcgcccgt gtgggccgca cggtgtgccc gaagtgccac
     2101 ctcgaggtgc gccgctgggc gcccgacgaa gtgatcgacg ccttgctcgg ggacatgccg
     2161 ggccgcgccg tgttcctggt ggccacgcgc gcggtcgaaa aaggcagcgg ggtcaagctg
     2221 ttcgaagagc tgattcgcga agggtacacg cgctaccgcg tcgccggcga cgtggtccgt
     2281 ttggaggccg cacccaaggg tgcggcgcgc atgaagtcga tcgacgtcgt gctcgaccgg
     2341 gtggagatcg acgagagccg gcgcgcgcgg ttgttggagg ccatcgagac cgcgtaccgg
     2401 atggccgacg gggtggtgcg cgtcgacgac gccgagaccg gcgaggcgcg ctcgttcacc
     2461 aacacgctcg cgtgcacgtc gtgcggccgc acgttcgagg agccccggcc gattctattc
     2521 tcgttcaaca cgcccttcgg cgcctgcccg cagtgccgcg gcttcgggag ccgcatggag
     2581 ttcgaccccg cgctggtggt gcccgacccc acgaagtcga tccgcgagca cgcggtggag
     2641 ccgtgggcca gcgagaagtt cgaatacttc tacgaccagc tggttcggtt ctgtcgtcgc
     2701 aaaaaaatcc ccatggacaa gccgtggtcc ggcctcaagg aggccgagcg gcgcgcgatc
     2761 atggacggcg agggcgagta cgtcggcgtg atgccgtttc tcgagaacat gcgccagaag
     2821 acctataaga aatatgcgcg cttcttcacg cggcggtacc tggcgttccg cgagtgccgc
     2881 gcgtgcgggg gaggaaggct gcgcgaggag gcgttcttcg tccgtctggg cggccgcacc
     2941 atccgcgacg tcgccgccat gtcgccgggc gaagcgctcg cgttcgtgcg cggcctctcg
     3001 ttcaacgagc gcgagcgcac ggtcgccaaa gacgtgctga tcgagctcga atcgaggctc
     3061 gagttcctgc tcgcggtggg gctgcactac ctcaccctcg accgtctcgc caagacgctc
     3121 tcgggcggcg agtcgcagcg catctcgctc gccaactcgc tgggctcgag cctgctcgat
     3181 gtgctctacg tcctcgacga gcccagcgtg ggactgcacc cgcgcgatac cgaccggctg
     3241 gtgcgcgtgc tcggcgacct gcgcgcgcgc ggcaacacgg tggtggtggt cgagcacgac
     3301 ctcgatataa ttcgcgccgc cgaccacgtc gtggacctgg gcccgggcgc cggcgaaggc
     3361 ggcggacgcg tggtctacca aggcccgccc gccgacgcca ctaccggcac gctgcgcttc
     3421 ctcgtcgaag ggcccccgat cgtgacccgc gccggcgcac gcgcgccgga ggcgttcatg
     3481 gtgctgcgcg gggtgcgtga acacaatctg cgcgacgccg acgtggcgct gccgctcggg
     3541 tcgttcacga gcgtcaccgg tgtatccggg tcgggcaaga gcacgctggt gtgcgacgtc
     3601 ctctacgacg cgctgcgcgc gaagaacggc cacggcggtg ctgcctatcg cgcgctcgag
     3661 ggtgccggcg cggtgcgcgg cgtgctgatg gttgaccagg ccccgatcgg aaagacgccg
     3721 cggtcgaatc cggtgacgta catcaaggcc ttcgcgctgg tgcgcgagat cttcgcggcg
     3781 cagaagaagg cggcgcggcg cg
//
LOCUS       DASPGK010000323         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1133764, whole genome shotgun sequence.
ACCESSION   DASPGK010000323 DASPGK010000000
VERSION     DASPGK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133764"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VEC56_09165"
     CDS             <1..1078
                     /locus_tag="VEC56_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXV14360.1"
                     /translation="FRLAIETVLGNEGLSRALTALPALLERNTDTRLLTKGVSTPANT
                     SSARRRSSAASWAGTSSAVKRKRRRAAGAWIAVASVLVVGGGAAGFFYWPQLRTLGFS
                     PSSAPASVQQADVGGGAGLGLDSALSGGIAVPADSLRLVVADSTATPIDTTATPGDAS
                     AVVASTESSGPRAPMVWTSPATSQPTAPAIDAPPATQPSEEAKPEKKVQEEPAKVFRE
                     GYLSITVDPSCEVWVNGKYRGDASPTLRLTLPTGRQTIECRHPKSETYMETIQIVSGE
                     LSRRNITLKRLRGIISLATTEGAELYIDGQLIGITPIMRPIEVEAGTHTVTIKKSNFY
                     AWTSEVVVEANEMLPLKITLSPRY"
     gene            complement(1075..2175)
                     /locus_tag="VEC56_09170"
     CDS             complement(1075..2175)
                     /locus_tag="VEC56_09170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV14361.1"
                     /translation="MGRLKTLERSGKRALMGAIGLLLRARPIDAAEFRSMVFQRILVI
                     RQHNQMGDMMLATPALRAIRESYPDASIGVVSSTLNQGVLVNSPYVDRVFTYDKRRPW
                     TTITMISRIRDQRYDLAIVLHTVSFSFTSLLLAVLSRARVRVGSTSRTIGTSLTGSYL
                     NFTLPLPDEAALSRMNEAEHNLFPLRAVGITTADIAPLLVPGEASERWADAAAAEYWR
                     AGTVRLAVHPGAGKAANIWPPERFAAVIDDLCATRAASLVIIEGPRDAQSVLDFQRAC
                     GSAGTVVRGRPITDVAALLQRADLVVCNDTGVMHVAAAAGARVLAVFGPTDPFRWAPR
                     CKNLRVVRAPGGNLRALTPEAVSKAAGEWLAG"
     gene            complement(2180..3106)
                     /gene="rsgA"
                     /locus_tag="VEC56_09175"
     CDS             complement(2180..3106)
                     /gene="rsgA"
                     /locus_tag="VEC56_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXV14362.1"
                     /translation="MQDRLENGLVVRVTGGDVWVRVGSAVLASSLRGRLRVKKDALQV
                     VAGDRVTVIRDPGGSGVTLEEVLPRASVLSRWVDRGGRERAIVANVERLFVVTSLSEP
                     PFRPVFADRVLAAAEWGRAPAALVLNKTDLAGRADLEAVSEVYRGCGYEVLPTCAATG
                     RGIDALERLIGDGVYAFVGESGVGKSSLINRLDPNVELVTQEVGERTGRGRHTTTSSQ
                     LFPFRTGFLADTPGMQTFGFPGRDETELSACFPEIARIDAPCRFHPCTHSHEPGCAVK
                     AAVEAGTIAPSRYQSFLAILGEIRAHTKEQSW"
     gene            3196..3666
                     /locus_tag="VEC56_09180"
     CDS             3196..3666
                     /locus_tag="VEC56_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXV14363.1"
                     /translation="MMTSQELASYFRETLCIFDLKAASKREALAEMVDAVCSEHDIKD
                     RGLILEMLLNRESLGSTAIGKGVAFPHGRTLAVRDLSVVYARSVRGVDFDSTDKKPTH
                     AFFLIIAPPQDRDNVYLQVLGRIVDIIKDDAARDRIAAAASYGELKTIIEGQGL"
     gene            3663..4022
                     /locus_tag="VEC56_09185"
     CDS             3663..4022
                     /locus_tag="VEC56_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14364.1"
                     /translation="MTDHPARKQDQARLLVALQDLDHMIRDAEDAKTAAQMQEMGFPM
                     DGIAEIKKARTELTEHIKPQLLNRYERVSKNYARIVVPVINELCTGCFAKVPTSFRYE
                     KNSVVTCESCGRILYFT"
     gene            4172..>5740
                     /locus_tag="VEC56_09190"
     CDS             4172..>5740
                     /locus_tag="VEC56_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14365.1"
                     /translation="MESSVRAFRRNLEPFEEERVAGPWRVAGHVDLVSERRYLVGWAP
                     LDATGRLDGDAIAFRCDAAVAIAPEVCAPALEVVVNNGRIEILSPYAAELNATTLPKA
                     GVEAAMSSIVDALARFESHAFFATEVAPELLAVGEEGVVTLLLGAYAVPPDALDAARD
                     GAWSHPRARGCQTHAAGLATIAQSLAALAEAPVAKRLRSLASEIAQGRIRSASEAAAC
                     WHGVAMPARVETSAPGDDADIDAIARAIASSQLVEVAGAAESGRTAVLERVLAHLRGA
                     GADVKWLDEWDASTPSRRKNAPREVARGTVWIVDDVEERPLLHSLILDHIFEMEKRAG
                     AGLVVATRSDAPDAFAASLRARAARGVHRVNTARVPAGTDDAILEALDSEARQVLELL
                     AVAAIPMPLDVVLSVMPGADQRVHRHLFALAGAELVAIESRRMPPGGSASLVLRVPRA
                     SVRRRVTERIPESRLRNLHRTIARIADERGNFPPLFAYQHLVEAGEAEDAASRAAAFV
                     KESTRAQRAPFLDAI"
BASE COUNT          926 a         2025 c         1925 g          864 t
ORIGIN      
        1 cttccgcctc gcgatcgaga ccgtgctggg caacgaggga ttgagtcgcg cgctcaccgc
       61 gctgcccgcg ctgctcgagc gcaacaccga cacgcgcctg ttgaccaagg gcgtgtcgac
      121 gccggccaac acgtcgtcgg cgcgccgccg gagctcggcg gcgtcgtggg ccgggaccag
      181 ctcggcggtc aagcgcaagc ggcgccgcgc ggccggggca tggatcgcgg tcgcgtcggt
      241 gctcgtggta ggtggcggcg cggccgggtt tttctactgg ccgcaactgc gcacgctcgg
      301 cttttcgccg tcgtcggcgc cggcgtcggt gcagcaagcg gacgttggcg gcggcgcggg
      361 tctgggactc gactccgcac tgagcggcgg cattgccgtt ccggccgatt cgctccgcct
      421 cgtggtcgcc gactctacgg ccacgcccat cgacacgaca gcgacccccg gcgacgcgtc
      481 cgcggtggtc gcgtccaccg agtcgagcgg gccacgcgca ccgatggtgt ggacctctcc
      541 cgccacgtcg cagccgaccg ctcccgcgat cgacgcgccc ccggccacgc aaccgagcga
      601 ggaagcgaag ccggagaaga aggtccaaga agagcccgcc aaggtgttcc gcgagggcta
      661 cctgtcgatc accgtggacc cgagctgcga ggtgtgggtg aacggcaagt accgcggcga
      721 cgcgtcgccc acgctgcggc tgacgctacc caccgggcgg cagaccatcg agtgccgcca
      781 cccgaagagc gagacctaca tggagacgat ccagatcgtc tcgggcgagt tgtcgcgccg
      841 caacatcacc ctcaagcggt tgcgcggcat catcagcctc gcgaccaccg agggcgccga
      901 gctctacatc gacggccagc tgatcggcat cacgcccatc atgcgaccga tcgaagtcga
      961 ggccggcacg cacaccgtga ccatcaagaa gtccaacttc tacgcgtgga cgtccgaggt
     1021 cgtggtggag gcgaacgaaa tgcttccgct gaagatcacg ttgagcccgc gctactagcc
     1081 cgcgagccac tcccctgccg ctttcgagac cgcctcgggt gtcagagccc gcaggttccc
     1141 acccggcgcg cgcacgacgc gaagattctt gcaacgcggc gcccaccgga acggatcggt
     1201 gggaccgaag accgccagca cgcgggcacc cgccgcggcg gcgacgtgca tcaccccggt
     1261 gtcgttgcac accaccagat ccgcgcgctg caagagtgcc gcgacgtcgg tgatcgggcg
     1321 tccgcgtacc accgttcccg cgctaccgca cgcgcgctgg aaatcgagga ccgactgcgc
     1381 gtcgcgcggc ccctcaatga tcacgagcga ggctgcgcgc gtcgcgcaca agtcgtcgat
     1441 cacggccgcg aatcgctcgg gcggccagat gttcgcggcc ttgcctgcgc ctgggtgcac
     1501 ggccaagcgc accgttcccg cacgccagta ctcggccgcg gccgcgtcgg cccagcgctc
     1561 gctcgcttcg cccggcacca acaggggcgc gatgtcggcg gtggtgatgc cgaccgcgcg
     1621 caggggaaaa aggttgtgtt cggcttcgtt catgcgggag agcgccgctt cgtcggggag
     1681 cgggagcgtg aagttcaggt agctgccggt gagcgaggtg ccgatcgtgc gcgatgtgga
     1741 tcccacgcgg acgcgcgccc gcgacagcac ggccaagagc aggctcgtga acgaaaacga
     1801 gaccgtgtgc agcacgatcg cgaggtcata acgctggtcg cggatgcgcg agatcatggt
     1861 aatggtggtc cacggccggc gtttgtcgta ggtgaacacg cggtcgacgt acgggctgtt
     1921 gacgaggaca ccctggttca acgtcgacga caccacgccg atggacgcgt ccggatacga
     1981 ctcgcgaatc gcgcgcagcg cgggtgtggc gagcatcatg tcgcccatct gattgtgctg
     2041 ccgaatgacg aggatgcgct ggaacaccat ggaccggaat tccgccgcgt cgatcggccg
     2101 cgcgcgcagg agcaggccga tggcgcccat gagcgcgcgc ttgccggatc gttcgagggt
     2161 cttcagtcgc cccacgggct caccacgact gctctttcgt gtgcgcgcga atctcgccca
     2221 ggatggcgag gaagctctgg taacgcgacg gcgcgatggt acccgcttcc accgcggcct
     2281 tgaccgcgca ccccggctcg tgcgaatgcg tgcacggatg gaagcggcac ggcgcgtcga
     2341 tgcgcgcgat ctccgggaag cacgccgaca actcggtctc gtcccggccc ggaaagccga
     2401 aggtctgcat gccgggcgtg tcggccagga atccggtgcg gaagggaaag agctgcgacg
     2461 aggtggtggt gtggcggccg cgcccggttc gttcgcccac ttcttgcgtc accaactcca
     2521 cgttggggtc gaggcggttg atgagcgacg atttccccac ccccgattcc cccacgaagg
     2581 catagacgcc gtcgccgatc agccgctcca aagcgtcgat gccccggccg gtggccgcac
     2641 aggtcggcag gacctcgtag ccgcagccgc ggtagacctc ggagaccgcc tcgaggtcgg
     2701 cccggcccgc gaggtcggtc ttgttgagga cgagggcggc gggcgcccgg ccccactcgg
     2761 cggccgccag cacccggtcg gcgaacaccg gccgaaaggg aggctcggac agcgaggtca
     2821 cgacgaagag tcgctcgacg ttggccacga tcgcgcgttc gcgcccgccc cgatcgaccc
     2881 agcgggagag caccgaggcg cgcgggagga cctcttccag ggtcacaccc gacccccccg
     2941 ggtcgcggat gaccgtgacc cggtcgcccg ccacgacctg gagggcgtcc tttttcaccc
     3001 gcaacctccc gcgcagcgac gatgccagga ccgccgagcc cacgcggacc cacacgtcgc
     3061 cgcccgtgac acgcacgacg aggccgtttt cgagtcgatc ttgcatgcag gttggtgttt
     3121 ggactagtat acggaccggg ttgccgggaa ttgcggttga gaatacccgc cggttctgat
     3181 attttccagg cgttcatgat gacgtcccaa gagctcgcgt catacttccg agaaaccctc
     3241 tgcattttcg atctcaaggc ggcctccaag cgcgaggcgc tcgccgagat ggtcgacgcg
     3301 gtgtgctccg agcacgatat caaagaccgc ggcctcatat tagaaatgct gctgaaccgg
     3361 gagagcctgg gcagcaccgc gatcggcaag ggcgtcgcgt tcccgcatgg gcgcacgctg
     3421 gccgtgcgcg acctctcggt ggtctacgcg cgctcggtgc gcggggtcga tttcgattcc
     3481 accgacaaga aacccacgca cgccttcttc ctcatcatcg ccccgcccca ggaccgcgat
     3541 aacgtctatc tgcaagtgct cggccgcatc gtcgacatca tcaaggacga cgccgcgcgc
     3601 gaccgtatcg ccgcggccgc gagttacgga gagctaaaga ccataattga agggcagggt
     3661 ttgtgaccga ccatccagcc aggaagcaag atcaggccag actcctcgtc gcactccagg
     3721 acctcgatca catgatccgc gacgcggagg acgcgaagac cgccgcccag atgcaggaga
     3781 tgggtttccc catggacggc atcgccgaga tcaagaaagc ccgcaccgag ctcaccgagc
     3841 acatcaagcc gcaattgttg aaccgctacg aacgcgtctc gaagaactac gcgcgcatcg
     3901 tggtgcccgt gatcaacgag ctctgcacgg ggtgcttcgc gaaggtcccg acctcgttcc
     3961 gctacgagaa gaactcggtc gtgacctgcg aaagctgcgg ccgcatcctc tatttcacct
     4021 agccttcccc gcgcctcgga aaaacccggg gcttgcgttt ctgcccagct ttggtaaaat
     4081 attttaacca aatcccggcc gtccgtccgc ccgttcgata attttcccgg cggcccgagt
     4141 ggccgcgcta gttgctgtaa ggagatacgc cgtggagtct tcggtacgcg ccttccgccg
     4201 gaacctcgag cccttcgaag aggagcgcgt cgcggggccc tggcgcgtgg ccgggcacgt
     4261 cgacttggtc agtgagcggc gctacttggt cggctgggcg ccgctcgacg cgaccggccg
     4321 cctcgacggc gacgccatcg cgttccgctg cgacgccgcg gtggccatcg cacccgaggt
     4381 gtgcgcaccc gcgctcgaag tcgtcgtgaa caacgggcgc atcgagatcc tctcccccta
     4441 cgccgccgag ctcaacgcca ccacgcttcc caaggccggc gtggaagccg cgatgtcgtc
     4501 gatcgtcgat gcgctggccc gcttcgaatc gcacgcgttc ttcgcgaccg aggtcgctcc
     4561 cgagctcctc gccgtcggcg aggaaggggt cgtcactctg ctcctcggcg cgtacgcggt
     4621 cccgcccgac gctctggatg ccgcgcgtga cggcgcgtgg tcgcacccgc gcgcgcgcgg
     4681 gtgccagacg cacgcggccg gtctcgcgac catcgcgcaa tcactcgccg cgctagccga
     4741 ggcaccggtg gcgaagcgcc tgcgctcgct cgcatcggag atcgcgcaag ggcggattcg
     4801 ctcggcgtcc gaagcggccg cgtgctggca tggggtcgcg atgcccgcgc gggtcgagac
     4861 atctgcaccc ggcgacgacg ccgacattga cgccatcgcg cgcgcgatcg cgtcgtcgca
     4921 gctggtcgag gtcgccggag cggccgagag cggccgcacc gcggtgctgg agcgcgtgct
     4981 cgcgcacctg cgcggcgccg gcgccgacgt caagtggctc gacgagtggg acgcgtcaac
     5041 cccgtcgcgg cgcaagaacg cgccccgcga ggtcgcccgc ggcacggtgt ggatcgtcga
     5101 cgatgtcgaa gagcgcccac tgctccattc gctcatcctc gaccacatct tcgagatgga
     5161 gaaacgcgcc ggcgcgggct tggtggtggc cacgcgctcc gacgcccccg acgccttcgc
     5221 ggcctcgttg cgcgcgcgcg ccgcgcgcgg tgtgcaccgc gtgaacaccg cgcgcgttcc
     5281 cgcgggcacc gacgacgcga tcttggaggc gctcgactcg gaagcgcgcc aggtgctcga
     5341 gctcctggcc gtcgccgcca tcccgatgcc gctcgatgtc gtgctatcgg tgatgcccgg
     5401 cgccgaccag cgcgtccatc gccacctgtt cgcgctcgcg ggcgccgagc tggtcgcgat
     5461 cgagtcgcgc cgcatgccgc ccggcggctc ggcgtcgctg gtgctgcgcg tcccgcgcgc
     5521 gtccgtgcgg cgccgtgtca ccgaacgcat ccccgaatcc cggcttcgca acctgcaccg
     5581 taccattgcg cgcatcgcgg acgagcgcgg caactttccg ccgctcttcg cttaccagca
     5641 cttggtcgag gcgggcgaag ccgaggacgc cgcgtcgcgc gccgcggcct tcgtcaagga
     5701 gtctacgcgc gcgcagcgtg cgcccttcct agacgccatc
//
LOCUS       DASPGK010000324         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_549387, whole genome shotgun sequence.
ACCESSION   DASPGK010000324 DASPGK010000000
VERSION     DASPGK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549387"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..141
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            396..2261
                     /locus_tag="VEC56_09195"
     CDS             396..2261
                     /locus_tag="VEC56_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV14366.1"
                     /translation="MRPRAGRVEDLTAVLSQLVGVRVTQYGGLGSFATVSIRGSSSSQ
                     VRTFLDGMPIDDPYLGVTNLSDLPLGGVERIEVYRGFSPPQLGGSAIGGAVSLITRDD
                     ASSGGGFFNAAEAALSAGSFDTRRENGSLFLRPGPVRVFAHGTYERSQGDFEFEDDNG
                     TPYNLDDDEVATRVNNDFEGWNGIARVSTDIPRVAEASLAYYDASRENGVPGLGSYQS
                     TTARSERRRQMGQARLEGVPLFANKQLRWWANGFYQRANEQFYDPDSDVSLLAQDTDN
                     TIDSYGGGARARWMVPRVPIALEATYLGQKEQYHPVNHLPQPTSGPDRWRHATTWSLC
                     ADVYLLEQTLVLTATQRWEENTDEFFDPPRFPWLPPTPQGVTTHRTDTPSFGVRYQPW
                     RWLGIKANAGRYYRLPTFLELFGNTGSVTGNALLEPEQGENRDVGVVLNFANLGAVRS
                     LLLEVSRFDNKAENLILFFPNSQYTVKPTNIGASRIRGWEVSAAAMWTDWEFAAGYTL
                     LDAEDTSDIPYYHGNELPSRPQDDVSASLSYAWRSLRATYEFHYMSANWLDRANLSET
                     PSRELHNLLVRVRTPVDGLALILEGQNLADERPVDVAGYPLPGRSLYTTLSYDYR"
BASE COUNT          406 a          746 c          765 g          387 t
ORIGIN      
        1 aagggaagag ggtgagaatc cctcgcggac ccgccactgt gattggcgag ccggccgccc
       61 acaccactgg gggaacccgg gaagggggcg gcggcgttcg agccatcagc caggatacct
      121 gccggatgtt gccaccctga ctcctcttcg aggaatgaga ggaacgggat gaggccatca
      181 atccccgaca tcgttctcgc ggacgaccgc tagccggcgg tctgctgctg cttggtgttc
      241 tgcttctgat atcggcggtt cccacgtggg tgccaggggc ggagcctgcc cgcggggatt
      301 cgctctacac gctgccggcg attattgtca cggcaccggg tccggatgcg cggtcggaga
      361 tcacgaaccg cccgggcttc gtggcgttgg ttgacatgag gccgcgcgcg gggcgggtcg
      421 aagatctcac ggcggtgctc tcgcagctgg tgggtgtgcg cgtgacgcag tacgggggcc
      481 tggggagctt cgccacggtc tccattagag gctcgtcgtc gtcgcaagtg cgtaccttct
      541 tagatgggat gccgatcgac gatccgtatc tgggcgtcac caatctctcc gatctcccgt
      601 tgggcggagt cgaacgcatt gaagtctatc gcggcttttc accgccgcag ctgggcggct
      661 ccgcgattgg tggcgcggtt tctcttataa cgcgcgatga cgcgtcctcg ggaggagggt
      721 tcttcaacgc cgccgaggcg gcgttgtccg cgggctcctt cgacacgcgg cgcgagaatg
      781 ggtcgctctt tctgcgaccg gggccggtgc gtgtgttcgc gcacggaact tacgagcgct
      841 cgcagggaga tttcgaattc gaagacgaca acggcacgcc ctacaacctc gacgacgacg
      901 aagtcgccac gcgcgtgaac aacgacttcg aggggtggaa cgggatcgcg cgcgtttcaa
      961 ccgacatccc gcgcgtcgcg gaagcctcgc tcgcctacta cgacgcgtcg cgcgagaacg
     1021 gggtcccggg cctgggatcg taccaatcga cgacggcgcg ctcggagcga cggagacaaa
     1081 tgggtcaagc gcgcttggaa ggcgtgccac tgttcgcgaa caagcaactg cgctggtggg
     1141 caaacgggtt ctaccaacgc gcgaacgagc agttctacga cccggactcc gacgtctcgc
     1201 tcttggcgca agacacggac aacacgatcg actcgtacgg gggcggcgcg cgtgcgcgct
     1261 ggatggtccc gcgggttccg atcgcgctgg aggcgacata cctcgggcag aaggagcaat
     1321 atcatcccgt caaccatctc ccgcagccga ccagcgggcc ggatcggtgg cggcacgcga
     1381 ccacgtggtc gctctgcgcg gacgtctacc tgctggagca aacgctggtg ctcacggcga
     1441 cgcagcgctg ggaggagaac acggacgagt tcttcgaccc gccgcgcttc ccgtggctcc
     1501 cgcccacacc gcagggggtc acgacgcacc gcaccgacac gccgtccttc ggtgtgcgct
     1561 atcaaccgtg gcgctggctc gggatcaagg cgaacgcggg gcgctattac cggctcccca
     1621 ccttcctcga gctcttcggc aacacgggct cggtgacggg caacgcgctg ctcgaacccg
     1681 agcaaggcga gaaccgcgac gtgggcgtcg tcttgaactt cgccaacctc ggcgcggtgc
     1741 ggtcgctcct actcgaagtg agccgcttcg acaacaaagc cgagaatctc atcctcttct
     1801 tcccgaactc gcagtacacg gtcaagccga cgaatatcgg cgcgtcgcgc attcgggggt
     1861 gggaggtgag cgcggcggcg atgtggacgg attgggaatt cgcggcgggc tacacgctcc
     1921 tcgacgcgga agacaccagc gacatcccct actaccacgg caacgagctg ccgtcgcgcc
     1981 cgcaagacga tgtcagcgcg tcgctctcgt acgcgtggcg cagcctgcgc gccacctacg
     2041 aattccacta catgagcgcc aactggctgg atcgcgccaa cctgagcgag acgccgtcgc
     2101 gcgagttgca caacctgctc gtgcgcgtgc gcacaccggt cgatgggctc gcgctgatac
     2161 tcgaaggaca aaacctggcg gacgagcggc ctgtggacgt ggcgggatac ccgctcccgg
     2221 gccgctcgct ctacaccacc ttgagctacg actaccgata ggaggcaccc ccatgaaaac
     2281 gctctcgatt ttgctcgtgc tggc
//
LOCUS       DASPGK010000325         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1603774, whole genome shotgun sequence.
ACCESSION   DASPGK010000325 DASPGK010000000
VERSION     DASPGK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603774"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..636)
                     /locus_tag="VEC56_09200"
     CDS             complement(67..636)
                     /locus_tag="VEC56_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HXV14367.1"
                     /translation="MVDTVTSVCAGVGLAAACGFRVFVPLLVLSIAGTLDVVPLAPDF
                     AWLATTPALLALATATVMEIAAYYVPWLDHALDVIATPAAVLAGVIASAAVLSDMTPV
                     LRWALAIVGGGGAAGAVQGATVLVRLKSGVATAGVGNPLVSTGETASSTVTSVLAVVV
                     PVIAFVLVVLTIVLLVTLARRSLRRGAGP"
     gene            complement(639..1229)
                     /locus_tag="VEC56_09205"
     CDS             complement(639..1229)
                     /locus_tag="VEC56_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV14368.1"
                     /translation="MKPKRKTEAKSPTANPEEGKLRLTPANRRDAAAWLAMRQDLYSD
                     VDDESHALEMDLILSSSDRACLLVRAPDDAAVGFIEITLRNAVDGCLGGPVGYIEGIY
                     LIPSWRGLGLGPAMVDAVAEWFRAQGCRAMATDAEIDNEEAQQFWDDLGFEETYRIVQ
                     YRKSLGAVPAAEPSPPKKRASAKGARKKAAAKPRRR"
     gene            complement(1226..1966)
                     /locus_tag="VEC56_09210"
     CDS             complement(1226..1966)
                     /locus_tag="VEC56_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXV14369.1"
                     /translation="MEGIATFIVVGFLAQLIDGTLGMAYGLSATTFLLAAGVSPAVAS
                     ASVHAAEVITTGVSGLAHWNSGNVSGYLVKRLVVPGMIGAATGAFILASVPGEKIKPL
                     VAGYLVVMGLFIVWKGLRRAMQHKEVRSHLIPLGLGGGFLDAIGGGGWGPIVVSNLVA
                     RGNHPRYSIGSVNFAEFFVASAASAAFFVTIGLSFWTAIVGLAIGGAIAAPIAAYLTR
                     RVPTRALMVVVGILIIALSVRTLYTVIR"
     gene            complement(2035..2853)
                     /locus_tag="VEC56_09215"
     CDS             complement(2035..2853)
                     /locus_tag="VEC56_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV14370.1"
                     /translation="MTSNEHEYVLGTDDAELLRLGLQHRLWSAQAFACWERAGVKPGA
                     TVLDVGAGPGFATLDLAQLVTPSGRIIAIDESERFLRHLAERARVLGLSHVETRAQDV
                     QALDVPAASVDVAYARWVLCFTPNPEDVVARVAAALKPGGAFAVQDYINWGALSVSPR
                     SDAFLRVMPAVGKSWRDHGGDWHVGDRLPAMMARRGLRVESITPLQRVARSSDPLWQW
                     PTTFFANFIPRLVEQGLVAEADWREMERDWQERSRDPNAFFWTPSMVEIIARRA"
     gene            2896..>3177
                     /locus_tag="VEC56_09220"
     CDS             2896..>3177
                     /locus_tag="VEC56_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14371.1"
                     /translation="MKASTIILSLLLAGPTAAQEPAPADSTRAPASAGSLDLGSAPLS
                     QGLLLSKYASRYRADAPAPALLTISLNPFAPQGYVPPTRFDAMLFGAGTA"
BASE COUNT          551 a         1124 c         1045 g          457 t
ORIGIN      
        1 cgcttcatcg cgcccgaagg tagcgaattc ccacacacgg cgtccagcgg ttcccgtgcg
       61 cgggtatcac ggtccagcgc cgcgtcgaag cgagcggcgg gcgagcgtca ccaagaggac
      121 gatggtcaag accaccagta cgaacgcgat cacgggcacc acgacggcca gcaccgacgt
      181 caccgtcgac gacgccgtct cgccggtgga gaccaagggg ttccccacgc cggcggtggc
      241 gacacccgac ttgagtcgca cgagcaccgt cgcaccctgc acggcacccg cagcaccccc
      301 gcctcccacg atcgcgagcg cccagcgcag caccggcgtc atatcggaga gcaccgccgc
      361 ggaggcgata actcccgcga gcaccgcggc cggggtggcg atcacatcga gcgcgtgatc
      421 gagccacggc acgtagtagg ccgcgatctc catcacggtg gcggtagcga gcgcgagcag
      481 cgcgggtgtc gtggcgagcc acgcgaagtc cggggcgagc ggcaccacgt cgagggtgcc
      541 ggcgatgctc aacacgagga gcggaacgaa gacacggaac ccgcacgcgg ccgcgagccc
      601 cacccccgcg cagacgctgg taacggtgtc gaccacggct agcggcggcg cggtttcgcc
      661 gcggccttct tgcgcgcacc cttcgccgac gcgcgcttct tgggcggcga cggttcggcc
      721 gccgggacgg cgcccagcga cttgcggtac tggacgatgc gatacgtctc ttcgaagccg
      781 aggtcgtccc agaactgttg tgcctcttcg ttgtcgatct cggcgtcggt ggccatggca
      841 cggcaccctt gcgcgcggaa ccactccgcg accgcgtcga ccatggccgg cccgagcccg
      901 agtccgcgcc agctggggat caggtagatg ccctcgatgt acccgaccgg tccgcccaag
      961 cacccatcga cggcgttgcg cagcgtgatc tcgatgaagc cgaccgccgc gtcgtcgggc
     1021 gcccgcacca agaggcacgc gcgatcgctc gacgacaaga tgaggtccat ctcgagcgcg
     1081 tgcgactcgt cgtcgacgtc ggagtagagg tcctgccgca tggcgagcca ggccgcggcg
     1141 tcgcgccggt tggccggggt cagccgcagc ttgccttctt cgggattggc cgtggggctt
     1201 ttcgcctccg tttttcgttt gggtttcatc gtatcaccgt gtacagggtt cgaacactca
     1261 acgcaatgat cagaattccg acgacgacca tgagggcgcg ggtcggcaca cgacgggtca
     1321 agtacgcggc gatgggtgcc gcgatggcgc cgccgatggc gagacccacg atggcggtcc
     1381 agaacgacaa accgatggtt acgaagaacg cggccgaggc ggccgaggcc acgaagaact
     1441 cggcgaagtt gaccgacccg atcgagtaac ggggatggtt gccgcgcgcg accaagttgg
     1501 agacgacgat cggtccccat ccgccaccgc cgatcgcgtc caagaacccc ccgcccaatc
     1561 cgagcggaat caggtgggag cgaacttcct tgtgctgcat ggcgcgccgc aatcccttcc
     1621 acacaatgaa caggcccatg acgaccaggt agccggccac gaggggcttg atcttctctc
     1681 ccggcaccga cgcgaggatg aaggcgccgg tcgcggcacc gatcataccc gggaccacca
     1741 gtcgcttgac caggtatccg ctcacgttgc ccgagttcca gtgcgcgagc ccggagacac
     1801 cggtcgtgat cacctcggcc gcgtgcacgc tggcgctcgc gaccgcgggc gaaacacccg
     1861 ccgcgagcaa aaaggtcgtg gcactcaagc cgtacgccat accgagggtt ccatcgatca
     1921 actgggccag gaacccgacg acgatgaagg tcgcgatgcc ttccatcggg gcattatacg
     1981 acaaggactt ccagggacgg ctatggtttc ggggcggtac aggaggaggc cgtgctaggc
     2041 ccttcgggcg atgatctcga ccatcgacgg ggtccagaag aacgcgttcg ggtcgcgcga
     2101 gcgctcctgc cagtcgcgct ccatctcgcg ccagtccgcc tccgcgacca ggccctgctc
     2161 gaccagacgc gggatgaaat tggcgaagaa ggtcgtaggc cactgccata ggggatcgct
     2221 cgaacgtgcg acgcgctgga gcggtgtgat ggactcgacg cgcagcccgc gccgcgccat
     2281 catcgcgggc aggcgatcgc cgacgtgcca atcgccgccg tggtcgcgcc agctcttccc
     2341 caccgccggc atcacgcgca ggaaggcgtc gcttcggggc gagaccgaaa gcgcccccca
     2401 gttgatgtaa tcctggaccg cgaaggcgcc gcccggcttg agggccgcgg ccacgcgcgc
     2461 gaccacgtcc tccgggttcg gtgtgaagca gagcacccag cgcgcgtagg cgacgtcgac
     2521 gctggccgcg ggcacatcga gcgcttgcac gtcctgcgcg cgcgtctcca cgtgcgagag
     2581 gccgagcacg cgcgcccgct ccgcgaggtg acgcaagaag cgctcggatt cgtcgatcgc
     2641 gatgatgcgc cccgacggcg tcaccaattg cgcgaggtcc agggtggcga agcccgggcc
     2701 cgcgcccacg tcgagcacgg tggcaccggg tttgaccccc gcgcgctccc aacatgcgaa
     2761 tgcctgggca ctccagagcc gatgctgcag gcccaggcgc aacaactcgg cgtcgtcggt
     2821 gccgagaacg tattcgtgtt cgttcgatgt catcgtgcta tactcgacgc gctgcgcacg
     2881 tcaaggagcc tgcccatgaa ggcgtcgacc atcatcctgt ccttgctgct cgccggtccc
     2941 accgccgcgc aagagcccgc gcccgccgac agcacccgtg cacccgcgag cgccggtagc
     3001 ctcgatctcg gctccgcgcc cctctcgcag ggcttgctcc tctcgaagta cgcgtctcgc
     3061 taccgcgcgg atgccccggc tcccgcgctt ctcacgatat cgctcaatcc gttcgcgccc
     3121 caaggatacg tccctccgac ccgcttcgac gcgatgctct tcggcgcggg aacggcc
//
LOCUS       DASPGK010000326         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1252089, whole genome shotgun sequence.
ACCESSION   DASPGK010000326 DASPGK010000000
VERSION     DASPGK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252089"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..779)
                     /locus_tag="VEC56_09225"
     CDS             complement(84..779)
                     /locus_tag="VEC56_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV14372.1"
                     /translation="MKRYAPIAILFACVVLAAPPAHAEGYAGFRVGGHRAVFTGGDAE
                     QLPWSRVGFTGGVFIGFDSGQHLGFRTDLLYTQKGGQGPEPTVEAEYLELLQGGALDY
                     SNSLIKIDYIEMAPLLVARFPLTGRFSIRGFVGPVLGLWINAEAAETLDQGGQEYHFD
                     VDLGDIVEHWEFGATIGVELNAAVGPYVVLLESRYGQGSRVFEDVGLLGEPLDFNASN
                     SGLSVMAGLMVPF"
     gene            complement(782..1507)
                     /locus_tag="VEC56_09230"
     CDS             complement(782..1507)
                     /locus_tag="VEC56_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HXV14373.1"
                     /translation="MCLFGAARAQTPSDAESPAGDPRFYGGARLSVTMSDVSSVQEHD
                     PRFGVGGGLFVAGTVWKSFDLRVEANYVQKGARLAFSRSAIEWQMDYLEIPVLLVWNI
                     IPKSKTSVELYGGMSYGIPVQQQVEQGENLGYDIEDFTEQPIFINQNTVLQINDVEDT
                     DLGFALGIGLSVPVGQVNFLVDARLTRSITDPVVAADFVTTIGEDEEAVTTTTVTDFS
                     NRLFSFYIGFSFPFGARAPAGSE"
     gene            1744..3099
                     /gene="glmM"
                     /locus_tag="VEC56_09235"
     CDS             1744..3099
                     /gene="glmM"
                     /locus_tag="VEC56_09235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXV14374.1"
                     /translation="MSSQLLFSVAGARGIVGQTIDADVVTRLTLAFCSTLPNGPVAVG
                     RDTRPSGESFACSVVGAVTSTGRDVIDLGIATTPTVEIVTERTDAVAGIIVTASHNPI
                     EWNALKFLDHRGIFITKESGAKVHAAYKEGRFVFADGKSTGKITDYTTAAREHIDAIL
                     SLDVIDVTAIRKRKFTVVLDCINGAGSVIAPQLLEALGVHVIPLNCVPDGNFYRDPEP
                     RPENLQDLADAVRKSRAHLGLATDPDADRLALVDENGRAISEEYTLALAADVVLAKHK
                     GPVVVNLSTSSWLDHVGKTHGVPVFRTPVGEANVVERMTRENAVVGGEGNGGVIYPAI
                     HAGRDAMLGMALILQLLASRNASLSAAVANLPPLLMTKDKVALRGAFDTGRISDALRP
                     KNPVTLDTQDGVKATFADGWIHLRVSNTEGIVRVIAEGPDSQKAAALQRIARDALDAS
                     W"
     gene            3140..>3817
                     /locus_tag="VEC56_09240"
     CDS             3140..>3817
                     /locus_tag="VEC56_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HXV14375.1"
                     /translation="MCGIIGYIGNNAAMPILLEGLKRLEYRGYDSAGVAILENGHLTV
                     EKTKGKISQLEGLVGLRKWKGTVGMAHTRWATHGEPNTENAHPVTDCTGDIAVVHNGI
                     IENYSALKQRLIKEGHLFTTQTDTEVIAHLVEKFFKTTGVLEEAVRLTVSQLEGTYGL
                     VIISKKEPDKLIGVRNGSPLIIGVASDENFLASDVSAILKHTQKVIYLDDHEMVTVSR
                     DGFVTKTI"
BASE COUNT          748 a         1247 c         1193 g          629 t
ORIGIN      
        1 accgagcggg gtttatttcg tggtgctgaa gagcaggggc gaacgcgtca cccgacgcgt
       61 cgtcctgctg cggtaaggag aggctagaat ggcaccatca ggccggccat cacactgagg
      121 ccgctgttgg atgcattgaa atcgagcggc tcgcccagca ggccgacgtc ttcgaaaacg
      181 cgggaaccct gcccgtagcg tgactcgagc aggacgacgt agggtcccac cgccgcgttg
      241 agctccactc cgatcgtcgc accgaactcc cagtgctcga cgatgtcccc cagatcgacg
      301 tcgaagtgat attcctgtcc gccctggtcc aatgtctctg cggcctcggc gttgatccac
      361 aggccaagaa ccgggcccac gaagccgcgg atcgagaagc gtcccgtcag cgggaaacgc
      421 gccaccaaga ggggagccat ctcgatgtag tcgatcttga ttagagaatt gctgtagtcg
      481 agcgccccgc cctggagcag ctcgaggtac tcggcttcga cggtcggttc gggaccctgt
      541 ccgcccttct gggtgtagag aagatcggtc cgaaaaccga ggtgctggcc ggaatcgaac
      601 ccgatgaaca cgccgccagt gaagccgacg cgcgaccacg gcagctgttc cgcgtcgccg
      661 ccggtgaaaa ccgcgcgatg gccgccgact cggaacccgg cgtaaccctc cgcgtgcgcg
      721 gggggcgcgg ccaacacgac gcacgcgaac agaatggcga tgggtgcgta acgcttcacg
      781 gctactccga cccggccggc gcgcgtgcgc cgaagggaaa cgagaatcca atatagaacg
      841 aaaacaagcg attcgaaaaa tcggtcaccg tcgtcgtcgt gacggcctcc tcgtcttcac
      901 cgatcgtggt cacgaagtcc gcggcgacga cggggtcggt tatgctcctc gttaggcgcg
      961 catcgaccag aaagttcact tgcccgaccg gcacgctcaa gccgatgccg agcgcgaacc
     1021 cgaggtccgt gtcctccacg tcgttgatct gaagcaccgt gttctggttg atgaagatcg
     1081 gctgttcggt gaaatcctcg atgtcgtagc cgagattctc gccctgctcg acctgctgct
     1141 ggaccggaat tccgtacgac atgccgccgt acaactcgac cgacgtcttc gacttcggga
     1201 tgatgttcca gaccaggagc accggaatct cgaggtagtc catctgccat tcgatcgcgc
     1261 tgcgcgagaa cgccaggcgc gcgcccttct ggacgtagtt ggcttcgacg cggaggtcga
     1321 acgacttcca cacggtgccc gcaacgaaga ggccgccgcc cacgccgaag cgcgggtcgt
     1381 gctcctggac cgaactcacg tcgctcatcg tgaccgagag acgcgcgccc ccgtaaaaac
     1441 ggggatcccc cgccggtgac tcggcgtcgc tcggcgtctg ggcccgcgcc gcgccgaaca
     1501 ggcacacgga cgcgactccc aggcacacga agagccgggc tccgcgaggg ccttgggaga
     1561 cgtgggttgt ggggcggaaa caatgggtcg aacggctacg actcatgaag cggcgaaact
     1621 agcatagccc tcggggggcg tcaagcttcg ttcgcgcgtc cctttcctgt tggctcgtga
     1681 cgggctctcg gcctatattc gggccgtctg atcgcttgtg ctgttagtgt cggaacccga
     1741 gccatgtcct cccaacttct ctttagcgtt gccggcgccc gcgggatcgt cggccagacc
     1801 atcgacgccg acgtcgtgac acggctgaca ctcgcgttct gctccaccct ccccaacggc
     1861 ccggtggcgg tgggacgcga cacgcgcccc agcggcgagt ccttcgcgtg ctcggtggtc
     1921 ggcgcggtga cgtcgaccgg ccgcgacgtg atcgacctgg gtattgcgac cacacccacc
     1981 gtggagatcg tcaccgagcg gaccgacgcg gtagccggca tcatcgttac cgcgagccac
     2041 aaccccatcg agtggaacgc actcaagttc ctcgaccacc gcggcatctt catcaccaag
     2101 gagtccggtg cgaaggtgca cgcggcatat aaggagggcc gcttcgtctt cgccgacggc
     2161 aagagcacgg ggaagatcac ggactacacg accgccgcac gcgaacacat cgacgcgatt
     2221 ctctccctcg atgtgatcga cgtcacggcg attcggaagc gaaagtttac cgtcgttctc
     2281 gattgcatca acggggcggg cagcgtcatc gcgccgcaac tgctcgaagc gctcggcgtc
     2341 cacgtgatcc cgctcaactg cgtccccgac ggaaacttct atcgcgaccc cgaaccgcgg
     2401 ccggagaatc tgcaagattt ggcggacgcg gtgaggaagt cgcgcgccca tttgggtctc
     2461 gcgaccgacc cggacgcgga tcgcctcgca ttggtcgacg agaacggccg cgcgatcagc
     2521 gaagagtaca cactggcgct cgcggccgac gtcgtgctcg ccaagcacaa aggcccggtg
     2581 gtcgtgaacc tctcgacctc gtcctggctc gaccacgtgg ggaagaccca cggcgtgccc
     2641 gtgtttcgaa ccccggtggg tgaggccaac gtggtcgagc gaatgacgcg cgagaacgcg
     2701 gtggtcggcg gcgagggcaa cggcggtgtc atctaccccg cgatccacgc cgggcgcgat
     2761 gccatgttgg ggatggcatt gatattgcag cttttagcat cccgcaacgc gagcctgtcg
     2821 gcggcggtgg cgaacctccc accgctgctc atgacgaagg acaaggtcgc cttgcgcggc
     2881 gcgttcgata ccggccggat ttcggatgca ctccggccaa agaatcctgt tacactcgac
     2941 acccaagatg gtgtgaaggc gacatttgcg gacgggtgga tccaccttcg cgtgtccaac
     3001 acggaaggaa tcgtccgcgt gatcgccgaa ggaccggact cccagaaggc ggcggccttg
     3061 cagcgaatcg cccgcgacgc tctcgacgcg tcgtggtgat tctccccagc gccggccacc
     3121 gaatcggagg aattcacgca tgtgcggaat catcggctac atcggcaaca acgcggcgat
     3181 gcccatcctc ctcgagggtt tgaagcgcct cgaataccgc ggctacgact ccgccggggt
     3241 cgccatcctg gagaacggcc acctcaccgt cgagaagacc aagggcaaga tctcgcagct
     3301 cgaggggctg gtgggacttc gcaagtggaa ggggaccgtc ggcatggcgc acacgcgctg
     3361 ggccacccac ggcgagccca acaccgaaaa cgcgcacccg gtcaccgact gcaccggcga
     3421 catcgcggtg gtccacaacg gcatcatcga gaactattcg gccttgaagc agcgcctcat
     3481 caaggaaggc cacctgttca cgacccagac cgacaccgaa gtgatcgcgc acttggtgga
     3541 gaagtttttt aagaccaccg gcgtgctcga ggaagcggtg cgtctgacgg tgtcacagct
     3601 cgaaggtacc tacggcctcg tcatcatcag caagaaggag cccgacaagc tgatcggcgt
     3661 gcgcaacggt tcgccgctca tcatcggcgt cgcgagcgac gagaactttc tcgcctccga
     3721 cgtgtcggcc atcttgaaac acacccagaa ggtcatctac ctcgacgatc acgaaatggt
     3781 gaccgtgagc cgcgacggct tcgtcaccaa gaccatc
//
LOCUS       DASPGK010000327         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_784270, whole genome shotgun sequence.
ACCESSION   DASPGK010000327 DASPGK010000000
VERSION     DASPGK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784270"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="VEC56_09245"
     CDS             complement(<1..2193)
                     /locus_tag="VEC56_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14376.1"
                     /translation="MLRALVTAFCAVSLTYSLAQSKRTTDTRIPTVSIAPTGPTDGSL
                     SPDARFAIAAANTTVLYSASFNLGASCAEQGWTTVDATAQPGTFWHVDDFAGANVDVG
                     DSYAPLAGAKSLWCGARAASSGDLCLYAVLPGYGDGWHQAWRTKTCLAVSGDLDVSFL
                     MRVDSEPQYDAAFLEYTDQCGAEYRDASWALLAGGTGVWDGVQNISHAASYDVGPGPV
                     EVRIRFASDGTYSDEDGDFDSHGGPVIIDDLDVEGLALEDFEDEAVGATSSDDWEADL
                     FPGFGDYFALFSGASLLQEDVCAKNLSCAWAAIASSTDTYACGGHPEQIAVPTGLGNG
                     GLYIDNSIQSPPIPLTGSGSRVNLQFSVYRDLAISRGVVYTWAVRGLENGCPDPWGSR
                     NTVYFGDQKDWKVDVFAIEDLIDLSASTHVQVRLGVFDRCIVSCGFTTTGDCHSHAPL
                     FDTVKVYRVDVGGPTFVVDDFDMFQDTFPADGSATGIGRADMARSITAAASPTIQPGD
                     SCVLICKDPLTAVSPSNPTGLADDLALGGKQVYAWVHVIDSGVPSVTKTGAALTDDPV
                     RYPFKDTQVADGKTWTRIRCDLAQASVGRFRVDLHDNLFEAGDVIEFFFGATNTLGET
                     NYCSGSALDYVQSDASVAAAAASEFTILPLDGFTGILYVDGMDGRGGQVFWDTAFGQI
                     GFEPDRYDVRGPASAVANRPSTRVTDVTTQLNGHYRKILWDCGDLSVGL"
     gene            complement(2229..>4671)
                     /locus_tag="VEC56_09250"
     CDS             complement(2229..>4671)
                     /locus_tag="VEC56_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14377.1"
                     /translation="SGTAFARSRTRGRIRWRGAPRVRLRFFADGGFSDEDANYDSHAG
                     PVVIDNLQVEGLSMETFEGESIGATASEDWEGAIVPGYGASYLWLFPGALQVQTDNCA
                     KDLTCLWAAILGSPETYACGGYPLQAAVPKGNSQGQYIHAQAWSPLFPITGTGTVINF
                     QFSVYRDMTLDSLVFYIWDVRAVIEGCEGPWRTRGFIYFGPQKDWLVNTFPVGDLIPA
                     EASHMRVRLGVIDQCGVWCGVFGTGACHGHAPLLDNAKVYRVDIFGPVWSTRDIDMFQ
                     DTFPVDGTDTGIGRADVALSITASASPTILPGDSARVIVSDPITAVPVTNPSGLMTDN
                     LGGTGNQAGTNGNRACYLYVHVLDSGVPSATKTGAILSGGPSYPFKDTVVADGKTWTR
                     IQCWLRVNSTSTFVVDLNDALFEAGDVVEFFWGAISTGGETSYCSGSALNYVQSDLDL
                     AAKVASEFTILPLDAPTSVLYVDGMDGRRAQAYWDTAFEQLGLLPHRFDVRGPTSSVS
                     NRPGTRVTDVATQLNGHYRVILWDAGDITQNLGDGTGAPEKSNDYAMVNQFLAGLASP
                     GGIYICGDDYFQGLNSATGSSAVTFKSTYLTFSYVTGNHRPSFGIAPIATGAAGGTFA
                     GDTFVVNGNCPLINDFDVVTPTGSSVTQMTYGAPTGTNSAVVSKTTGNARVLASGFSF
                     IYLRDDDEDGVMDRAQHLHDILAFLGETTDQPTDTAPVYTNRLEQNYPNPFNPQTTIG
                     FSLKERGRVRIDVFNVAGEHVRTLLDETRAAGAHTDVRWDGKDGSGRSVASGVYFCRL
                     VADRFSQTRKMVLVQ"
BASE COUNT          825 a         1461 c         1587 g          798 t
ORIGIN      
        1 gaggccgacc gacaggtctc cgcaatccca aagaattttg cgatagtgac cgtttagctg
       61 cgtcgttacg tcggtgacgc gcgtactcgg ccggttggcg accgccgacg cggggccgcg
      121 cacgtcatag cggtcgggct cgaaaccaat ctgcccgaat gcggtgtccc aaaacacctg
      181 gccaccgcga ccgtccatgc cgtcgacata gaggataccg gtgaatccat ccagcggcag
      241 gatcgtgaac tctgaagccg ccgcggcggc gacggaggcg tcggactgga cgtagtcgag
      301 ggcgctgccg gagcaatagt tggtttcgcc gagcgtgttg gtcgcaccga agaagaattc
      361 gatcacgtcg ccggcttcga acaagttgtc atgaaggtcg acgcggaacc gtccgacgct
      421 cgcctgcgcg agatcgcagc gtatacgagt ccatgtcttt ccgtccgcga cctgggtgtc
      481 cttgaaggga taacgcaccg gatcatcggt cagcgcggcg ccggtcttgg tgacactcgg
      541 cacgccgctg tcgatcacgt ggacccacgc gtacacctgc ttgcccccga gtgcgaggtc
      601 gtcggcgagg ccggtgggat tcgacggtga caccgcggtc agcggatcct tgcagatgag
      661 gacgcacgaa tcgccgggct ggatggtcgg gctcgcggcc gcggtgatcg agcgcgccat
      721 gtcggcgcgc ccgatgccgg tggcggagcc atcggccggg aacgtgtcct ggaacatatc
      781 aaaatcgtcg accacgaagg tcggcccgcc gacgtcgacg cggtagacct tgacggtgtc
      841 gaacagcggc gcgtggctgt gacagtcgcc ggtcgtcgtg aagccgcagc tcacgatgca
      901 tcgatcgaaa acgccgagac gtacttgcac atgcgtcgag gcggagagat cgatgaggtc
      961 ctcgatcgcg aacacgtcga ccttccagtc cttctggtcg ccgaagtaca cggtattgcg
     1021 gctgccccaa gggtccgggc aaccgttttc gaggccacgc accgcccacg tgtagacgac
     1081 accccgagat atcgcgaggt ctcggtacac ggagaactgc aagttcacgc gcgagccgct
     1141 ccccgtgagc gggatgggag gcgactggat cgagttatcg atgtacagtc cgccgttgcc
     1201 gaggccggtg ggcacggcga tctgctccgg atgcccgccg cacgcgtacg tatccgtcga
     1261 gctcgcgatg gccgcccacg cgcagctcaa gttcttcgcg cacacgtcct cttgcaggag
     1321 ggaggcgccc gagaacaatg cgaagtagtc gccgaatccc gggaacagat ccgcttccca
     1381 gtcgtccgac gatgtcgcgc ccaccgcctc gtcctcgaag tcctcgagtg ccagcccttc
     1441 gacgtcgagg tcgtcgatga tgaccggacc gccgtggctg tcgaagtcgc cgtcttcgtc
     1501 ggagtaggtg ccgtcggagg cgaagcgaat gcgaacttcg acgggaccgg gaccgacatc
     1561 gtaggaggcc gcgtgcgaga tattctgaac gccgtcccac acgccggtcc cgcccgcgag
     1621 cagcgcccac gaggcgtcac ggtactcggc gccgcactgg tcggtgtact cgaggaacgc
     1681 ggcgtcgtac tgcggctcgg agtcgacgcg catgaggaag gagacgtcga gatcgccgct
     1741 cacggcgagg cacgtcttcg tgcgccacgc ctgatgccat ccgtcgccgt agcccgggag
     1801 gacggcgtac agacacaggt cacccgatga cgcggcgcgc gcgccgcacc aaagcgactt
     1861 tgccccggcg aggggagcgt acgagtcgcc gacgtcgacg ttcgcccccg cgaagtcgtc
     1921 cacgtgccag aacgtgcccg gctgggcggt cgcatcgacc gtggtccagc cctgctcggc
     1981 acacgacgcg ccgaggttga agctggcgga atagagcacg gtggtattgg cggccgcgat
     2041 cgcgaagcgc gcgtcgggcg atagcgaacc gtcggtcggg ccggtgggag cgattgagac
     2101 ggtcggtatc cgggtgtcgg tggtccgctt ggattgagcg agagaatagg tgagcgaaac
     2161 cgcgcaaaag gcggttacca atgcccttag catcgtgcgc cctccaactg gccggctggt
     2221 cggggccctt actgcacgag taccatcttc ctcgtctggg agaaccggtc ggcgacgagt
     2281 cgacaaaagt ataccccgct tgcgaccgat cgaccagacc cgtccttgcc gtcccagcgg
     2341 acatcggtgt gcgcgccggc cgcgcgcgtc tcgtctagga gggttcgcac gtgctcgccg
     2401 gccacgttga acacgtcgat tctcacccgc ccgcgttcct tcaacgagaa gccgatcgtg
     2461 gtttggggat tgaaggggtt gggatagttc tgttcgaggc ggttggtgta aaccggcgcc
     2521 gtgtcggtcg gctgatctgt cgtttctccc aggaacgcga ggatatcgtg caggtgctgg
     2581 gcgcgatcca tcacgccgtc ctcgtcgtcg tcgcggagat aaatgaagct gaagccgctc
     2641 gcgagcacgc gcgcgttgcc ggtggtcttc gagacgacgg cgctattggt gccggtcggg
     2701 gcgccgtagg tcatctgcgt caccgatgag ccggtcggcg tcacgacgtc gaagtcgttg
     2761 atcaacggac aattgccgtt gaccacgaag gtgtcgccgg cgaacgtccc acccgccgca
     2821 ccggttgcga tcggcgcgat gccgaagctg gggcggtggt tgccggtgac gtacgagaat
     2881 gtgaggtacg tcgacttgaa cgtcaccgcg gaagaacccg tggcggagtt caagccctgg
     2941 aagtagtcgt cgccgcaaat gtagatcccg cccgggctgg cgaggccggc caggaactgg
     3001 ttgaccatcg cgtagtcgtt gctcttctcg ggcgcaccgg tcccgtcgcc gaggttctgc
     3061 gtgatgtcgc ccgcgtccca cagaatgacg cgatagtggc cgttgagttg ggtcgcgaca
     3121 tcggtgacgc gcgtgccggg gcggttcgac acgctcgaag tggggccgcg gacgtcgaaa
     3181 cgatgcggga ggagcccgag ctgctcgaac gcggtatccc agtacgcctg cgcgcggcgt
     3241 ccatccatcc cgtcgacgta gagaacgctg gtcggcgcgt cgagggggag gatcgtgaac
     3301 tcggaggcga ccttggcggc aaggtcgagg tcgctctgga cgtaattgag cgcgctgccg
     3361 gaacagtagc tcgtctcccc gcccgtgctg atggcgcccc agaagaactc gaccacgtct
     3421 ccggcttcga agagggcgtc gttcaagtcc acgacgaagg tgctcgttga attgacgcgc
     3481 agccagcact ggatgcgcgt ccaggtcttg ccatcggcga ccacggtgtc cttgaacggg
     3541 tacgacggcc cgccggagag aatcgcgccc gtcttggtag cacttggaac accgctgtcg
     3601 agcacgtgga cgtagaggta gcaagctctg ttgccgttgg tcccggcctg gttgcccgtg
     3661 ccgccgaggt tgtcggtcat gagcccactc ggattcgtga cgggaacggc ggtgatggga
     3721 tcgctcacga tgacgcgcgc cgagtcgccg gggagaatcg tcggactcgc gctcgccgtg
     3781 atcgagagtg caacgtcggc gcgtccgata ccggtgtcgg tcccatcgac gggaaacgtg
     3841 tcctggaaca tgtcgatgtc gcgcgtgctc cagaccgggc cgaagatgtc gacccggtac
     3901 accttggcgt tgtcgagcag cggcgcgtgg ccgtggcacg ctccggtccc gaacacgccg
     3961 caccacacgc cgcactggtc gatgacaccc aagcgcacgc gcatgtggga ggcttcggcc
     4021 ggaatcaggt caccgaccgg gaacgtgttg acgagccagt ccttttgagg cccgaaatag
     4081 atgaacccgc gtgtgcgcca cggtccctcg cagccctcga tcaccgcccg cacgtcccaa
     4141 atgtagaaga cgaggctatc gagggtcatg tcgcggtaga cggagaactg gaagttgatc
     4201 acggtgccgg taccggttat cggaaacagc ggtgaccacg cttgtgcgtg gatatactgg
     4261 ccctgcgagt tgcccttggg caccgcggcc tgtaatggat acccgccgca cgcgtacgtc
     4321 tccggtgagc cgaggatcgc ggcccacagg caggttaggt ctttcgcgca attgtccgtc
     4381 tgaacttgca gcgcaccggg gaacagccac aggtaggagg cgccgtagcc ggggacgatc
     4441 gcgccctccc agtcctcgga cgcagtggca ccgatggact cgccctcgaa tgtctccatt
     4501 gagaggccct cgacctgcag gttgtcgatc acgaccggac ccgcgtggct gtcgtagttc
     4561 gcgtcttcgt cggagaatcc tccgtcggcg aagaagcgaa ggcggacccg cggggctccc
     4621 cgccaccgaa tacgaccccg cgtgcgcgat cgagcgaatg ccgtcccact c
//
LOCUS       DASPGK010000328         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_901466, whole genome shotgun sequence.
ACCESSION   DASPGK010000328 DASPGK010000000
VERSION     DASPGK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901466"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VEC56_09255"
     CDS             <1..1414
                     /locus_tag="VEC56_09255"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXV14378.1"
                     /translation="TLDIPTLREMVLVHDDAFAKVGVAACILLFIGACGKSAQIPLYV
                     WLPDAMAGPTPVSALIHAATMVTAGVYMIARLNFLYDIAPAAAAVVAVIGAATAIFAA
                     TIGFAQRDIKKVLAYSTVSQLGYMFLAVGVGAYAAGVFHLLTHAFFKACLFLGAGSVI
                     HGLSGEQDMFKMGQLRKHMKITWATFLVSALAIAGIPPLAGFFSKDEILWGTFNAHVG
                     PEWLPRALWIVGLATAGMTAFYVFRAVFLTFHGKDNVSHEAKHHLHESPPVMTMPLII
                     LAVGAALAGFLGVPAALGGSNAFHHWLEPVFAGHVGGHDIGVGMASAVSHATGAASTI
                     AAATEGHNTDLEMILMGVSVLVAIVGILVAHAIYVRAPGGAEWVTRSLGPIYTLVANK
                     FYVDELYERALVRPGYAIADKLMYRVIDSGIIEGIVNGLGITARLFGAGMRLMQSGVV
                     RTYAFFMLIGFLYLVYELVK"
     gene            1415..2935
                     /locus_tag="VEC56_09260"
     CDS             1415..2935
                     /locus_tag="VEC56_09260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXV14379.1"
                     /translation="MDTLSLVTFLPLFGAIAIAMMPARAHAMIRWFALLLSTGVFVYS
                     LQLVQGFQTNADMQMTVDADWLPSLGISYKIGIDGISLFLVMLVTFLTPITILGCFNS
                     IEERVKEFFIAMLVLETAMIGAFVALDLFLFYVFWEAMLIPMYFMIGIWGGERRIYAT
                     VKFFIYTMIGSLLMLVAILYVVHQHRVQTGELTFDLALLLGTALSSQQQWMLFLAFAL
                     AFAIKVPMFPFHTWLPDAHVEAPTPGSVILAGVLLKMGTYGFIRFAMPMFPEAAIRSI
                     PYIAALSIIGIIYGACVAMVQQDVKKLVAYSSVSHLGFVMLGLFALNVQAVQGAMLGM
                     INHGISTGALFLLVGVIYERRHTRMISEYGGLTKVMPLYATVFMIVALSSIGLPPLNG
                     FVHEFLILVGAFQTNAWWGILAATGVVLGAIYMLWMFQRVFFGEVTNEKNAGLKDLTP
                     REVLVFVPILVMIFWMGIYSKPFTERMEPAITKFVDQMTSVQQAMNDEEPRVEESN"
     gene            2935..>3981
                     /locus_tag="VEC56_09265"
     CDS             2935..>3981
                     /locus_tag="VEC56_09265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXV14380.1"
                     /translation="MPQFNAEIARLLSPLFILAGGACLLLAWSAIDEKAARAQAWFAG
                     LVHVAALWSIAMLWNAEGWPILNGMLIVDRFALFFAGICVVSSLATVLVSSGYLHRFN
                     IHRGEYYALLLLSTAGMFVLVTAYDLMSVFLGIELMSLAIYVIVGFRRRDFLANEAAM
                     KYFLLGAFAIAFFLYGMSIIYGLCGTMNFKGIVVEATSRELIKDPPFLFAIALLLVGF
                     VFKVSAVPFHMWVPDVYQGAPSPVTGYMAGAVKAASFAAFLRLFYMSFMPARAQWVDI
                     VVVLAIATMTLGNVVALVQRNVKRMLAYSSIAHAGYILIGMAALGIDNTKAASGVMFY
                     LVVYAFMTLGAFALI"
BASE COUNT          597 a         1277 c         1249 g          858 t
ORIGIN      
        1 gaccctcgat attcccaccc tgcgcgagat ggtgttggtc cacgacgacg ccttcgccaa
       61 ggtgggagtg gcggcctgca ttctgctgtt catcggcgcg tgcggaaagt cggcgcagat
      121 cccgctgtac gtgtggctcc ccgacgccat ggcgggcccg accccggtca gcgccttgat
      181 ccacgccgcc accatggtca cggccggggt gtacatgatc gcgcgcttga acttcctgta
      241 cgacattgcg cccgccgccg cggcggtcgt ggccgtgatc ggtgccgcca ccgccatctt
      301 cgccgcgacc atcggcttcg cgcagcgcga catcaagaag gtgctcgcgt actcgaccgt
      361 gagccagctc ggctacatgt tcctcgccgt gggcgtgggc gcctacgcgg cgggcgtgtt
      421 ccatttgcta acccacgcgt tcttcaaggc gtgtctgttc ctcggcgcgg gctcggtgat
      481 ccacggtctc tcgggcgagc aggacatgtt caagatggga cagctgcgca agcacatgaa
      541 gattacctgg gcgacgttcc tcgtctccgc gctcgcgatc gccgggattc cgccgctcgc
      601 gggattcttc tcgaaggatg aaatcttgtg gggcaccttc aacgcgcacg tcggccccga
      661 gtggctgccg cgcgcgttgt ggatcgtggg actcgcgacc gcggggatga ccgcgttcta
      721 cgtgttccgc gcggtgttcc tcacctttca cggcaaggac aacgtctcgc acgaagccaa
      781 gcaccacctg cacgagtcgc cgccggtgat gacgatgccg ctcattatct tggcggtggg
      841 cgcggcgctc gcgggcttcc tgggtgtgcc ggcggcgcta ggcggcagca acgcgttcca
      901 ccattggctc gagcccgtgt tcgcggggca cgtgggcggg cacgacatcg gcgtgggcat
      961 ggcctccgcg gtgtcgcacg ccaccggggc cgcgagcacc atcgccgccg cgaccgaagg
     1021 acacaacacc gacctcgaga tgatcctgat gggcgtctcg gtgctggtcg cgatcgtggg
     1081 tatcctcgtc gcgcacgcga tctacgtccg cgcccccggg ggcgccgagt gggttacgcg
     1141 ctcgctgggt cccatctaca cgctggtcgc gaacaagttc tacgtcgacg agctctacga
     1201 gcgcgccctc gttcgccccg gatacgcgat cgcggacaag ctcatgtacc gcgtgatcga
     1261 ttccgggatc atcgagggca tcgtcaacgg gctcggcatc acggcgcgcc tgttcggcgc
     1321 cgggatgcgg ctcatgcagt cgggtgtcgt gcgcacctac gcgttcttca tgctgattgg
     1381 cttcctgtac ttggtatacg agttggtgaa gtaagtggat accctgagcc tggtcacgtt
     1441 cctgccgctc ttcggcgcca tcgcaatcgc gatgatgccg gcgcgcgcgc acgcaatgat
     1501 tcgctggttc gcgctgctct tgtcgaccgg tgtgttcgtg tattcgctcc aattggtgca
     1561 gggcttccag accaacgccg acatgcagat gaccgtcgac gcggactggc tgccctcgct
     1621 cggcatctcg tacaaaatcg gcatcgacgg catcagcctg ttcctggtga tgttggtgac
     1681 gttcctcacc cccatcacca tcctcgggtg cttcaattcg atcgaagagc gcgtgaagga
     1741 gttcttcatc gccatgctgg tgctggagac ggcgatgatc ggcgccttcg tggcgctcga
     1801 cctgttcctc ttctacgtgt tctgggaagc gatgctgatc ccgatgtact tcatgatcgg
     1861 catctggggc ggcgagcggc gcatctacgc caccgtgaag ttcttcatct acacgatgat
     1921 cggctcgctc ttgatgctgg tcgcgatcct gtacgtcgtc caccagcacc gcgtccagac
     1981 cggcgagctg accttcgacc tggcgctcct cttgggcacg gcactatcgt cgcagcagca
     2041 gtggatgctg ttcttggcct tcgcgctcgc gttcgccatc aaggtgccga tgttcccgtt
     2101 ccacacctgg ctccccgacg cgcacgtcga agcacccacg cccggctcgg tgatcctggc
     2161 cggagtactc ctcaagatgg gaacctacgg cttcattcgc ttcgccatgc cgatgttccc
     2221 ggaggcggcc attcgctcga ttccgtacat cgcggcgctc tccatcatcg ggatcattta
     2281 cggcgcgtgc gtcgccatgg tgcagcagga cgtgaagaag ctggtggcgt attcgtcggt
     2341 gtcgcacctg ggcttcgtca tgctggggct tttcgccttg aacgtccaag cggtgcaggg
     2401 cgcgatgctg ggcatgatca accacggtat ttcgaccggc gcgctctttc tattggtcgg
     2461 ggtcatctac gaacgacgtc atacccgcat gatctccgag tacggcgggc tgaccaaggt
     2521 gatgccgctc tacgcgaccg tgttcatgat cgtggcgctc tcgtcgatcg ggctcccgcc
     2581 cttgaacggc ttcgtgcacg agttcttgat cttggtgggt gcgttccaga ccaacgcgtg
     2641 gtggggaatc ctggctgcga ccggcgtggt gctgggagcg atctacatgc tgtggatgtt
     2701 ccagcgcgtg ttcttcggcg aagtgaccaa tgagaagaac gcgggcttga aggacctcac
     2761 accgcgcgag gtgctggtct tcgtccccat tctcgtgatg atcttctgga tgggcatcta
     2821 ctcgaagccc ttcaccgagc gcatggagcc cgcgatcacg aaattcgtcg accagatgac
     2881 gagcgtgcag caggcgatga acgacgaaga gccgcgcgtc gaggagagca actgatgccg
     2941 cagttcaacg ccgagatcgc ccggctcctc tcgccgctgt tcattctggc gggcggcgcg
     3001 tgcctcttgc tcgcgtggtc ggccatcgac gagaaggccg cgcgcgcgca ggcgtggttc
     3061 gcggggctcg tgcacgtggc ggccctgtgg agcatcgcga tgctgtggaa cgccgagggc
     3121 tggccgatct tgaatggcat gctgatcgtc gatcgctttg cgctcttctt cgcgggcatc
     3181 tgcgtggtct cgtcgctcgc gaccgtcctg gtctcatccg gatatctcca tcgattcaac
     3241 atccaccgcg gcgagtacta cgcgctcttg ctcctctcga ccgcgggcat gttcgtcctg
     3301 gtcaccgcct acgatctgat gtcggtgttc ctcggcatcg agctcatgag cctcgccatc
     3361 tacgtcatcg tcgggttccg ccgccgcgac ttcctggcca acgaggcggc catgaagtac
     3421 ttcctcttgg gcgccttcgc gatcgcgttc tttttgtacg ggatgtcgat catctacggc
     3481 ctgtgcggga cgatgaactt caagggcatc gtcgtcgaag cgaccagccg cgaattgatc
     3541 aaggatccgc cgtttctctt tgccatcgcg cttctcttgg tcggcttcgt gttcaaggtg
     3601 agcgcggtgc cgttccacat gtgggtgccc gatgtctacc aaggcgcgcc gtcgccggtg
     3661 accggataca tggcgggtgc ggtcaaggcg gcctccttcg ccgcgttcct tcgcttgttc
     3721 tacatgagct tcatgccggc gcgcgcacag tgggtggaca tcgtggtggt actcgcgatt
     3781 gcgaccatga cgctgggcaa cgtggttgcc ctcgtgcagc gcaacgtcaa gcgcatgctc
     3841 gcgtactcga gcatcgcgca cgccggctac atcctgatcg gcatggcggc cctcggcatc
     3901 gacaacacca aagccgcgtc gggtgtcatg ttctacctgg tcgtgtacgc cttcatgacc
     3961 ctgggcgcct tcgcgctcat c
//
LOCUS       DASPGK010000329         7553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1017538, whole genome shotgun sequence.
ACCESSION   DASPGK010000329 DASPGK010000000
VERSION     DASPGK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017538"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3051
                     /locus_tag="VEC56_09270"
     CDS             <1..3051
                     /locus_tag="VEC56_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14381.1"
                     /translation="IYAQRLNAGGAAQWTASGLPICNSAQDQSDPQIVLDDVGGAIIV
                     WSDRRSGAQDIYAQRVAPDGTPSWTANGVAVCTASNNQWYPRMVSDGAGGAIITWVDY
                     RSGTPNNTDVYAQRIDAAGVVQWTADGVAICTPTSTQFPPTIATDGNGGAIITWHSAA
                     TPVDAGDIYAQRVNSAGAVQWTANGVAVCTDPSDQSYPVIVSDGSNGAIIVWSDQRGE
                     WDLYAQRVNSAGAVQWNPAGVAVCAAAGAQFDATYPGIIRDGLGGAIVTWGDLRSGSY
                     DVYAQRMSGAGAAQWTADGVAVCTATEGQEMPAIVQDGSGGAIISWFDGRSGNLDIYA
                     QRLSMAGSPQWTPDGVAVCTAAGDQAWPVMFANDGGAVVVWDDRRSGRDIYAQRIQSD
                     GQVVSFTVNSTADVYVTGTLRSAIAQANAIVGPQVIRFDIPGAGPHVIDVMVQGLPAV
                     TDALTIDGFTQPGASPNTNPFGSPSNAQIKIQISGSSFAAGLHFQALGTLRGVAISGF
                     NPACVLVEAAGVAIEGNYIGTDASGLAAGANPQVDGIRILAPGACLVGGVTPAARNVI
                     CNSLSAGIQIDGAGGVEVYGNYIGVRSDAISPLSNWSGVRIMNGATNARVGSSSIAPP
                     FNPAEGNLIMGNTHYGVLVEGSGTISNAIVGNTMVSNTMGLIDLGADGFDVNDAGDGD
                     TGPNQRQNYPEFTSAVGNQITGTMTGAPYQPLFLHFYWSPACCIQAAHFIGGATIHLN
                     ASGSASFAVAPPAPVPPGTFINATATDMFGNTSEVSVPIQHLNTSSGPSQLVNLVDAN
                     GAIRGRATFDNVNTVGNTFVENPYTPPVPVSGYSIGSPNDPQIYFNIFTDALYTGGVD
                     VCLSYDENSIPGPEEDLALLHYDGSMWVDVTTSRDPWSNEICGRVTSLSPFVIGAITA
                     TAVDDTPLPESFALHANVPNPFNPITTIAYDIPDGGANVNITIYDVAGRRVRTLVDEH
                     RPAGVFSVQWNGDDDRGQRVASGVYFYRMRAGAFVDTKKMVLLK"
     gene            3100..3852
                     /locus_tag="VEC56_09275"
     CDS             3100..3852
                     /locus_tag="VEC56_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV14382.1"
                     /translation="MPHDQTPTDASMRDFWDQKARENAMYYISSYRAYDQQDPAEFWK
                     WGGILAEKFLGESGCRFTGEEAMLEIGCGVGRMTKYFAGRFRHVTALDVSPEMIRQAQ
                     ENLAGIDNVRLDVGNGSDLTAYPDASFDFVFSYIVFQHIPDPRVTLTYLREAGRVLRP
                     GGACYVQINTMPRVSPLRVALRRWKRALLRRTSAPASSATSGPRDLDHPAWQGSRVTM
                     TDVTAALRTGRMRIENVQGEGTQYTWVLARRS"
     gene            3946..7461
                     /locus_tag="VEC56_09280"
     CDS             3946..7461
                     /locus_tag="VEC56_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXV14383.1"
                     /translation="MPATRFLIGMDVGSTTVKAIVVDLASDRILWQDYHRHETKQPEK
                     VLEFLKRFDDEVDGLSRAVAEKEARVFITGSGGMNIAPRIGAKFVQEVNAVCLAVEKM
                     HPSAGSVIELGGQDAKIIVFKEDPETGRKKKIPSMNDKCAGGTGAVIDKINAKLRIPP
                     EELCQQGYDGIKLHHVAGKCGVFAETDINGLQKQGIPTSELMASLFEAIIGQNLSVLT
                     RGHTLRPEVILLGGPNTYIKGMREAWQHNIPVTWREREVAIPEGVDPKSLIVVPQNAQ
                     YYAAIGAVEYGKDEDEGVGTYKGWADLEDYITNGRKQSRAKASAGLSKSSDDLAAFKE
                     RYRPEPFDAARFAPGEVVRAFIGLDGGSTSTKAILLSTEKEVLAKSYQLSKGNPIKDT
                     IEVIGDIQRHVESQGARLEVLGVGTTGYAKDVLRDVLGADAAIVETVAHAESALHFYD
                     DVDVICDVGGQDIKIMVLNGGSVKDFKLNTQCSAGNGYFLQSTAGEFGVPVENFADTA
                     FGAEQMPTFGYGCAVFMQSDIVDFQRQGWTAGEIMAGLAAVLPKNIWLYVAQIPNLAK
                     LGKRFVLQGGTQRNLAAVKSQVDFIESRFRCNGDGPEVIIHKHCGESGAIGAALEAAR
                     LWENGRTTSFVGLDAVQVISYTATTSEDTRCHFCKNECLRTFIDVEASRVIKRDQGEV
                     DTAPATAGARKSKVPLATGARRLIVNNSCEKGLVEDVESMREIKKDLDARLKAAPNFV
                     EKSARDVFKSFKPESIADAPPKYAFTSAQKARVETIKKRPDFRIGIPRMLNQYSTNPL
                     FSAYFESLGIRPHNLVYSDYTTEELYKEGAKRGSIDPCFPSKLGIPHVHNLLFKHHEK
                     KPLDVIFFPMIDDLNSPLANTQDCRACPTVTATPEAVKAAFTKEGDWFAKKGIKYLCP
                     LVNISQKHLFSRQMFAAFADLLGVSEKENERAVEAGYKALAEYEGSIRREARAVLDEL
                     EAEQKIGIVVLGRPYHNDPGINHEILVEFQKLGYPVFTQDSLPIDDDILDRLFGDEVR
                     AGDVSSPMDITDVWKNAYSENTSRKVWAAKYTARHPNLVALELSNFKCGHDAPIYSVV
                     EEIIERSGTPYFSFKDLDENRPAGSIKIRVETIGYFLKRYREDKMRDEWRERVAEHEA
                     AAAAAPESRPDDELPERESEAFAAA"
BASE COUNT         1468 a         2424 c         2400 g         1261 t
ORIGIN      
        1 atctatgcgc aacgcctcaa cgccggcggt gcggcgcaat ggacggcaag cgggctgccc
       61 atctgcaaca gcgcacaaga tcagtcggac ccgcagattg ttctggacga cgtcggcggt
      121 gccatcatcg tctggtccga cagacggtcc ggcgcgcaag acatctacgc gcaacgtgtc
      181 gcgcccgatg gaacgccgtc gtggacggcc aacggtgtgg ccgtttgtac cgcttcgaac
      241 aatcagtggt atccacggat ggtttcggac ggagccggcg gcgccatcat cacatgggtg
      301 gattatcggt ccggcactcc caataacacc gatgtttatg cccagcgcat cgacgcagcc
      361 ggtgtggtgc agtggaccgc ggacggcgtc gccatctgca cgccaacgtc gacacagttc
      421 cccccaacca ttgctaccga cggtaacggc ggtgcgatca tcacgtggca cagcgcagcg
      481 acaccggtgg atgccggcga catctacgcg caacgggtca actcggccgg tgcggtgcag
      541 tggaccgcga acggtgtggc agtgtgcacg gacccgagcg accagtcgta tcctgtgatc
      601 gtctcggatg ggtcgaacgg cgccatcatc gtatggtcgg accaacgcgg tgaatgggac
      661 ctctacgctc aacgcgtcaa ctccgcgggc gcggtgcagt ggaatcccgc cggtgtggcg
      721 gtctgcgccg cggcgggcgc ccaattcgac gcaacgtacc caggcatcat tcgggatgga
      781 ttggggggag ccatcgttac gtggggcgac ctgcgcagtg gttcgtacga tgtctacgcc
      841 cagcgcatga gtggagccgg tgccgcgcag tggacagccg acggcgtcgc ggtgtgcaca
      901 gcgacggagg gccaagaaat gcccgcgatc gttcaagacg gttccggcgg cgccattatc
      961 tcgtggttcg acggtcgctc gggcaacctc gacatctacg cgcagcggtt gagcatggcg
     1021 ggctctccgc agtggacacc ggacggagtc gcagtgtgca ccgccgccgg cgatcaggcc
     1081 tggcccgtca tgttcgcgaa cgatggtggc gccgtggtgg tgtgggacga ccggcggtcc
     1141 gggcgtgaca tctacgccca gcgcattcaa agcgacggcc aggtggtttc gttcaccgtc
     1201 aattcgaccg ccgacgtcta cgtgaccggc accctgcgca gcgccatcgc ccaggcgaat
     1261 gcgatcgtgg gaccgcaggt gatccgcttc gatatccccg gcgccgggcc gcatgtgatc
     1321 gacgtgatgg tccagggcct gcccgccgtc accgatgcac tcaccatcga cgggttcacg
     1381 cagccgggcg cgtcgcccaa taccaatcca ttcgggagcc cgtcgaacgc gcagatcaag
     1441 attcagattt ccgggtcaag ttttgcggcg gggcttcatt ttcaagcgtt gggcacgctg
     1501 cgcggcgtcg ccatcagcgg cttcaacccg gcctgcgttc tggtcgaagc agccggtgtc
     1561 gcgatcgaag gcaactacat cggtaccgat gcgtcggggc tggccgctgg cgcgaaccct
     1621 caggtcgacg gcatccgcat cctcgccccc ggcgcctgcc tcgtgggcgg ggtcacaccg
     1681 gccgcacgca atgtgatctg caattcctta tcggcgggca tccagatcga cggtgccggg
     1741 ggcgtggagg tatatggcaa ttacatcggc gtgaggtcgg atgcaatctc gcccctctcg
     1801 aattggtcag gcgttcgaat catgaacggt gccaccaatg cgcgcgtcgg gtcgagttca
     1861 atcgcgccgc cttttaatcc cgccgagggc aacctcatca tggggaacac gcactacggc
     1921 gtgctcgtgg aaggctccgg cacaatttcg aacgcgatcg tcggaaacac gatggtctcg
     1981 aatacgatgg gtttgatcga tctgggcgcg gacggatttg atgtaaacga cgcgggcgac
     2041 ggcgacacgg ggccgaacca gcggcagaac taccccgaat tcacttcggc cgttggcaat
     2101 cagatcaccg ggaccatgac cggggcaccg taccagccac tgtttctcca tttctactgg
     2161 tcgcctgcgt gctgcatcca ggcggcgcac ttcatcggcg gagcgactat tcatctgaac
     2221 gcaagcggct ccgcttcgtt tgcggttgca ccgcccgcac ccgtgccgcc cggcaccttc
     2281 atcaacgcga ccgcgaccga catgttcggc aacacgtccg aagtatcggt ccccatccag
     2341 cacctgaaca cgtcgtctgg accatcgcag ctcgtcaacc tcgtcgacgc gaacggtgcg
     2401 attcgcggga gggccacgtt cgacaacgtg aacaccgtcg gcaacacgtt cgtcgagaat
     2461 ccctacactc cgccggttcc ggtgtcgggc tattcgatcg gcagtcccaa cgacccccag
     2521 atctacttca acattttcac ggacgcgttg tacacaggag gcgtcgacgt ctgtctcagc
     2581 tacgacgaga acagcattcc cggtcccgaa gaggacctgg cgctcctgca ctacgacggc
     2641 tcgatgtggg tcgacgtgac cacgagccgc gacccgtgga gcaacgagat ctgcggtcgc
     2701 gtgacctcgc tctccccgtt cgtgatcggt gcgatcacgg ccacggctgt cgacgatacc
     2761 ccgttgccgg agtcgttcgc gctgcacgcg aatgttccca accccttcaa tccgattacc
     2821 acgatcgcgt acgacatccc agacggcggc gcgaacgtga acatcacgat ctatgacgtc
     2881 gccggccgtc gcgtgcgaac gctggtggat gaacaccgac cggccggcgt tttctcggtg
     2941 caatggaacg gcgacgacga ccgcggtcag cgggtcgcgt cgggcgtgta cttctatcgg
     3001 atgcgcgcgg gggccttcgt cgataccaag aagatggtgc tgttgaagta gaggggaatc
     3061 cgccacgcag tagcgacgcg gtcgtgctaa catggagcga tgccgcacga ccaaacgccg
     3121 accgacgcct cgatgcgcga cttctgggac cagaaggcgc gcgagaacgc gatgtactac
     3181 atctcgtcgt accgggcgta tgaccagcag gacccggcgg agttctggaa gtggggaggg
     3241 atcctcgcgg agaagttcct gggggaatcg ggctgtcgct tcaccggcga ggaagcgatg
     3301 ctcgagatcg ggtgcggtgt ggggcggatg acgaagtact tcgcggggcg ttttcgccac
     3361 gtgaccgccc tcgacgtctc gccggagatg attcggcagg cgcaagagaa tctcgccggc
     3421 attgacaacg tccgtctcga tgtcgggaac ggatcggatc tgaccgccta tccggacgcg
     3481 agcttcgact tcgtgttttc gtatatcgtc ttccagcaca ttcccgaccc gcgcgtcacg
     3541 ctgacgtacc tccgcgaagc cggtcgcgtg ctgcgacccg ggggcgcgtg ctatgtccag
     3601 atcaatacca tgccgcgcgt ctcgccgctg cgtgtcgcgc tcaggagatg gaagcgggcg
     3661 ctcttgcggc gtacgtctgc gcccgcgagt tccgcgacga gcggcccgcg cgacctcgat
     3721 cacccggcgt ggcagggatc acgggtcacg atgaccgacg tcaccgccgc cttgcggacg
     3781 ggtaggatgc gaatcgaaaa cgtccaaggc gaaggcacgc agtacacatg ggtcctggcg
     3841 cggcgctcgt gagcagtcgg ccggccgtcg cccgccccgc ccgatagaag acggcgcgtc
     3901 cactattctg ttgcctcctc ggccggcctc gcccatactc ccgccatgcc cgcgacccga
     3961 ttcctcatcg gcatggacgt cggctcgacg accgtcaagg cgatcgtcgt ggatctcgca
     4021 tccgaccgga tcctgtggca ggactaccac cgccacgaaa ccaagcagcc cgaaaaggtc
     4081 ctcgagttcc tgaagcgctt cgacgacgaa gtggacggac tctcgcgcgc agtcgccgag
     4141 aaggaagccc gcgtcttcat caccggcagc ggcggcatga acatcgcgcc gcgcatcgga
     4201 gcgaagttcg tgcaggaagt caacgcggtc tgcctcgcgg tggaaaagat gcaccccagc
     4261 gcgggctcgg tcatcgagct cggcggccag gacgcgaaga tcatcgtctt caaggaagac
     4321 cccgagaccg gccgcaagaa gaagatcccg tcgatgaacg acaagtgcgc ggggggcacc
     4381 ggcgcggtca tcgacaagat caacgccaag ctgcgcatcc cgcccgaaga gctgtgccag
     4441 cagggctacg acggcatcaa gctccaccac gtggcgggca aatgcggcgt gttcgcggag
     4501 actgacatca acggcttgca gaagcagggc atcccgacca gcgagttgat ggcgagtctg
     4561 ttcgaggcca tcatcggtca gaatctctcc gtcttgacgc gcggccacac gcttcgcccc
     4621 gaagtcatcc tactcggcgg ccccaacacg tacattaagg gcatgcgcga agcgtggcag
     4681 cacaacattc ccgtcacgtg gcgcgaacgc gaggtcgcaa ttcccgaggg ggtggacccc
     4741 aagtcactga tcgtggtgcc gcagaacgcg cagtactacg ccgccatcgg cgcggtggag
     4801 tacggcaagg acgaggacga aggtgtcggc acctataaag gatgggccga cctcgaggat
     4861 tacatcacca acggccgcaa gcagtcgcgc gccaaggcca gcgcggggtt gagcaagtcc
     4921 tccgacgact tggcggcgtt caaggaacgc taccggccgg agccgttcga cgcggcgcgc
     4981 ttcgcgcccg gcgaagtggt gcgcgcgttc atcggcttgg acggcggctc cacgtcgacg
     5041 aaggccatct tgttgagcac ggagaaagaa gtgctggcga agtcctacca gctctccaag
     5101 ggcaacccga tcaaggacac catcgaagtg atcggcgaca tccagcgcca cgtcgagtcg
     5161 caaggcgcgc gtttggaggt gctgggggtc ggcaccacgg gctacgccaa ggacgtcctg
     5221 cgcgacgtgc tcggggccga cgccgccatc gtcgaaaccg tcgcgcacgc ggagtcggcg
     5281 ctccacttct acgacgacgt ggacgtgatc tgcgacgtcg gcggccagga catcaagatc
     5341 atggtcctca acggcgggag cgtgaaggac ttcaaactca atacgcagtg ttccgcgggc
     5401 aacggctatt tcttgcagag cacggcgggc gagttcggcg tgccggtcga gaacttcgcc
     5461 gacaccgcat tcggcgccga gcagatgccg accttcggat acggctgcgc ggtgttcatg
     5521 cagagcgaca tcgtcgactt ccagcgccag ggctggaccg cgggcgagat catggccggc
     5581 ttggccgcgg tcttgccgaa gaacatctgg ctctacgtcg cgcagatacc caacctagcc
     5641 aagctgggga agcggttcgt gctgcaaggc ggaacccagc gcaacctcgc ggccgtcaag
     5701 tctcaggtcg acttcatcga gtcgcgcttc cgttgcaacg gcgacggtcc cgaggtgatc
     5761 atccacaagc actgcggcga aagcggtgcc atcggtgccg cgctggaggc cgcgcgcttg
     5821 tgggagaacg ggcgcacgac cagttttgtc ggcctcgacg cggtgcaggt gatctcctac
     5881 accgcgacca cctccgaaga cacgcgttgc cacttctgca agaacgagtg cctgcgtacc
     5941 ttcatcgacg tcgaggcgtc gcgcgtgatc aagcgcgacc agggcgaggt cgataccgcg
     6001 ccggcgaccg cgggtgcgcg caagtcgaag gtgccgctcg caaccggcgc gcgccgcctg
     6061 atcgtcaaca actcgtgcga gaagggcctg gtggaggacg tcgagtcgat gcgcgagatc
     6121 aaaaaggacc tggatgcgcg gctgaaagcc gcgcccaatt tcgtcgaaaa gagcgcgcgt
     6181 gacgtgttca agagcttcaa acccgagagc attgcggatg ctccgcccaa gtacgcgttc
     6241 acttcggcgc agaaggcgcg cgtcgagacg atcaagaagc gccccgattt ccgcatcggc
     6301 atcccgcgca tgctgaacca gtacagcacc aacccgctgt tcagcgccta cttcgagtca
     6361 ttggggatcc gcccccacaa tctcgtgtac tccgattaca cgaccgaaga gctctacaaa
     6421 gagggtgcca agcgcggttc catcgacccg tgtttcccga gcaaactcgg cattccgcac
     6481 gtgcacaatc tcttgttcaa gcatcacgag aagaagccgc tggatgtgat cttcttcccg
     6541 atgatcgacg acttgaacag tccgctcgcg aacacgcagg actgccgtgc gtgcccgacc
     6601 gtcaccgcta cgccggaggc ggtcaaggcg gccttcacca aggaaggcga ttggttcgcg
     6661 aagaagggca tcaaatatct ctgtcccctg gtcaacatct cgcagaagca tttgttctcg
     6721 cgccagatgt tcgcggcgtt cgcggacctc ttgggtgtat cggaaaaaga gaacgagcgc
     6781 gcggtggaag cgggttacaa ggcgctcgcc gagtacgagg gctccatcag acgagaagcg
     6841 cgcgccgtgc tcgacgagct cgaagccgag cagaagatcg gcatcgtcgt attggggcgc
     6901 ccgtaccaca acgaccccgg catcaaccac gagatcctgg tcgagttcca gaagctcggc
     6961 tatcccgtct tcacgcagga ctcgctcccc atcgacgacg acatcctgga tcgtttgttc
     7021 ggcgacgaag tccgcgccgg cgacgtctcg agcccgatgg acatcaccga cgtgtggaag
     7081 aacgcctaca gcgagaacac gagccgaaag gtatgggccg ccaagtacac cgcgcgccac
     7141 ccgaacttgg tcgccctcga gctgtcgaac ttcaaatgcg gccacgacgc ccccatctac
     7201 tcggtggtcg aggagatcat cgagcgctcg ggcacgccgt acttctcctt caaggatctc
     7261 gacgagaacc ggcccgccgg atcgatcaag attcgcgtgg agacgatcgg gtacttcctc
     7321 aagcgctacc gcgaagacaa aatgcgcgac gagtggcgtg aacgcgtcgc cgagcacgag
     7381 gccgcggccg cggcggcccc ggaatcccgg cccgacgacg agctgccgga gcgcgaatcc
     7441 gaagcgttcg cagcggcgtg aacccgagcg gcgcaatcta cgagtacgta ccgggttcgc
     7501 cctgacggtc gaactcagac accccgtaat tccttgccca ccccttgaca att
//
LOCUS       DASPGK010000330         6635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1253085, whole genome shotgun sequence.
ACCESSION   DASPGK010000330 DASPGK010000000
VERSION     DASPGK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253085"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VEC56_09285"
     CDS             complement(<1..241)
                     /locus_tag="VEC56_09285"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXV14384.1"
                     /translation="MILCVDVGNTASKFALVRGSRVVRRAVVGSDAGPRDLARACARV
                     VREASSLEAAALSSVRPQATEAVVRAIVRVSTLYPI"
     gene            complement(238..1047)
                     /locus_tag="VEC56_09290"
     CDS             complement(238..1047)
                     /locus_tag="VEC56_09290"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXV14385.1"
                     /translation="MPLPFPSAGPLPADVRRRLATAYFGRRAYFYPETDSTNDVALSL
                     ASAGEAEGTTVVADYQRRGRGRRAHTWSSPAGKDLLFSVILRPEGDARHALPVTLVTA
                     TAISVTLSKLLDVDVMVKWPNDVVTGSGKLAGILAESASSGGRLSHVVVGIGVNVNAS
                     EADFAGDLRGLAASCRTLSGVEWDRAELLADLLGTIEAYYDRFKRDGFGTLSSAYEAR
                     LAQANRVVSFERDGARWSGRVAGVSPDGALVVRLDDGRRTELFSEHVEVIA"
     gene            complement(1047..1934)
                     /gene="nadC"
                     /locus_tag="VEC56_09295"
     CDS             complement(1047..1934)
                     /gene="nadC"
                     /locus_tag="VEC56_09295"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXV14386.1"
                     /translation="MTAPHGEAQPLDAALVASRVAAALSEDAAGHDATNAFLALGDES
                     ARAEIRVASATVACGVDVAREVFFQVDARIRFDAHTRDGAHLEAGARVCGLAGPARSL
                     LSGERTALNFLQRLCGVATLASRFVAAVGGTGVVILDTRKTTPLWRDLEKYAVRCGGA
                     RNHRHDLGAMILVKDNHVRAIGGRSALVARIAQSPRAPFVEVEVDSLEFLRELLASPA
                     ARNVDRLMLDNFTPERVAEAMADVVAYRARGARLEVEVSGGVTLDTVRAFAQAGVDFI
                     SVGALTHSAIAAPMSLEFL"
     gene            complement(1931..3958)
                     /gene="uvrB"
                     /locus_tag="VEC56_09300"
     CDS             complement(1931..3958)
                     /gene="uvrB"
                     /locus_tag="VEC56_09300"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXV14387.1"
                     /translation="MAPGFDLVSHYRPTGDQPQAIRALVQGLRDHTRDQVLLGVTGSG
                     KTFTIANVIAEVGMPTLVISHNKTLAAQLYGEFKGFFPHNAVEYFVSYYDYYQPEAFV
                     PSTNTYIAKDASINDDIDRLRVRATSSLMTRKDVIVVSSVSCIYGLGSPEMVRAMTIE
                     MAAGDSLDREDLLRSLIDIQYSRNDAAFTRGTFRVRGDVVEIRPAYEEEAIRIEFFGD
                     TVESISIADPLTGKRIRTLETLTLFPAKHFVTPEDVIKRAAREIRDELSTRVVELREL
                     GKLVEAQRLESRTLYDIEMLEEVGYCSGIENYARYLSGRKPGERPLTLLDFFPSEFLC
                     VVDESHVTLPQVGGMYHGDRSRKVTLVEHGFRLPSALDNRPLTFDEFDALVPRFIHVS
                     ATPSEFELARTRGEVVEQVIRPTGLVDPDIVVRPVEGQIDDLVEEIRTCAENGERVLV
                     TTLTKRMSEELTDYLLSLGIRVRYLHSDIDALERVEILRGLRLGEFDALVGINLLREG
                     LDLPEVSLVAVCDADKEGFLRSERSLIQTAGRAARHVSGRVIFYADQVTDSMRRAIDE
                     TRRRRALQLAYNTEHGITPRGIIKSLDEVALSTAIADAKRKDGADVIVDAGLPSEALA
                     AAIEAEMLREARDLNYEKAASLRDRLDEIRLALAATENRTAPRRLRGKSGE"
     gene            complement(3964..4419)
                     /locus_tag="VEC56_09305"
     CDS             complement(3964..4419)
                     /locus_tag="VEC56_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV14388.1"
                     /translation="MNNEGKTIGEMLRAAREEKNLTVEQANRETRISVQTIRSLERDD
                     FGAFPSETYLKGFLRNYAEFLGLDGNALWSMVASRSSGGSGTGPSWEIESALKEEKLG
                     PPPVIRRLILPLLVLLVVLFVWMWLREKRRADRVEQSAVEAVVEIGAAG"
     gene            complement(4467..>6635)
                     /locus_tag="VEC56_09310"
     CDS             complement(4467..>6635)
                     /locus_tag="VEC56_09310"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXV14389.1"
                     /translation="IYRGDYIVNWCPVDQTAISDEEVQHEERESKLYWIRYPSPHGEV
                     VVATTRPETMLGDTGVAVSPEDAAKRHLVGKTCTLPIIGRELPIVADAAVQKEFGSGF
                     VKVTPAHDPVDYGIGKRHNLATVVVIDRTGKMTAQAGADFAGLDRFEARAKVIERLEH
                     DGLVVKVEPHRHAVGKHDRCGTVIEPLLSTQWFVKMAPLAEPAVRAAETEAITFYPER
                     WRNVYMSWMTNIRDWCISRQLWWGHRIPVWYCDDCDHLTVSRTDPTRCEGCGGAKIRQ
                     DEDVLDTWFSSWLWTFSPLGWPDEDAPDLKRYHPTSVLVTGSEIIFFWVARMIMASLE
                     FMREVPFRHVFLTGIVRDGQGRKMSKSLGNSPDPLDIIDKWGTDAFRFTLSMLSPPGK
                     DVFFDEAKLELGRNFGNKMWQASRLILGAVDKEGKPLFKENANGDFTSTLAFRPEYAW
                     EDRWILSALSRCASDTNRCFEEWRLNEGAARVYDFFWHDFCDWYLELAKVRLYGEGDK
                     RTVLAVMVHVLGESLKLMHPLMPYITEEIASALPTSNGRLLLEHAYPQGDGRALDAEA
                     EGRMTVLRDVVTSVRNVRAAYRVNPGLKIPVRVRAHEPKASLVREATDGIVRLAGVAT
                     LEVGPDVAKEKGSAATPIGDLEVVIPLAGVVDVEAERARLTREREKVDAELSEVARKL
                     ANEGFVAKAKPEVVERERARRERLETELSKLVESLGLLGH"
BASE COUNT         1068 a         2292 c         2206 g         1069 t
ORIGIN      
        1 cgatcgggta cagcgtcgac acccgcacga tggctcgcac cacggcctcg gtggcctgcg
       61 gccgcaccga cgacagcgcg gcggcctcca gcgaggatgc ctcccgcacc acgcgcgcgc
      121 acgcgcgcgc caggtcgcgc gggccggcgt cgctcccgac caccgcgcgg cgtaccaccc
      181 gcgagcctcg caccagcgcg aacttgctcg cggtgttccc cacgtcgacg cacaggatca
      241 cgcgatcacc tcgacgtgct cgctgaagag ctcggtcctg cgtccgtcgt cgagacgaac
      301 cacgagcgcg ccgtccggcg acacacccgc cacccgcccc gaccagcgcg ccccgtcgcg
      361 ctcgaacgag accacccggt tcgcttgcgc gagccgggct tcgtacgcgg aggatagcgt
      421 gccgaagccg tcgcgcttga agcggtcgta atacgcctcg atggtcccca agagatcggc
      481 caggagctcg gcgcgatccc actcgacgcc ggagagggtg cggcacgacg ccgccagccc
      541 gcgcagatcg cccgcgaagt ccgcctcgct cgcgttgacg ttgacgccga tgccgaccac
      601 gacgtgcgag aggcgcccgc cgctcgaggc ggactcggcc aggatgcccg cgagcttgcc
      661 gctccccgtc acgacgtcgt tgggccactt gaccatgacg tcgacgtcga ggagcttcga
      721 gagcgtgacc gaaatggcgg tcgcggtcac cagtgtgacc ggcagcgcgt ggcgcgcgtc
      781 gccttcggga cggaggatca cggagaacaa aaggtccttt ccggccgggc tcgaccacgt
      841 gtgcgcgcgc cggccgcggc cgcgccgctg gtagtcggcc acgaccgtgg tgccttccgc
      901 ttcccccgcg ctcgcgagcg agagtgccac gtcgttggtc gagtccgtct cgggatagaa
      961 gtacgcacgc cggccgaagt acgcggtggc caggcgccga cgcacgtcgg cgggcaacgg
     1021 cccggcggac gggaacggca gcggcattac aggaactcca ggctcatcgg agccgcgatc
     1081 gcggagtgcg tcaaggcgcc gacggagatg aagtccacgc ccgcttgcgc gaacgcgcgc
     1141 accgtgtcca gcgtgacgcc gcccgagacc tcgacctcga ggcgcgcccc gcgcgcgcgg
     1201 tacgcgacga cgtcggccat cgcctccgcg acccgttcgg gcgtgaagtt gtcgagcatc
     1261 aggcggtcga cgttgcgcgc ggccggcgac gccaagagct cgcgcaggaa ctccagcgaa
     1321 tccacctcga cttcgacgaa gggcgcgcgt ggcgactgtg cgatgcgcgc aaccagcgca
     1381 gagcgccccc cgattgcgcg cacatggttg tccttgacca ggatcatggc acccaggtcg
     1441 tggcgatggt tgcgggcgcc gccgcagcgg accgcgtact tctccaggtc gcgccacagc
     1501 ggtgtggtct tgcgggtgtc gaggatgacc acaccggtcc cccccaccgc ggcaacgaaa
     1561 cgcgacgcca gggtggcaac gccgcacagt cgctgcagga aattgagcgc ggtgcgctcg
     1621 cccgaaagga gagaccgcgc gggacccgcg agtccgcaca cgcgcgcccc ggcctcgagg
     1681 tgggcgccgt cccgggtatg cgcgtcgaaa cggatgcgcg cgtcgacttg aaagaagacc
     1741 tcgcgcgcga cgtcgacgcc gcacgccacg gtcgcgctcg ccacgcggat ctcggcgcgc
     1801 gccgattcgt cgccgagcgc gagaaacgcg ttggtggcgt cgtggcccgc cgcgtcctcg
     1861 gacagcgcgg cggccacccg gctcgcgacc agggccgcgt cgagcggctg tgcctcaccg
     1921 tgcggggcgg tcattcgccg ctctttccgc gcagtcgccg cggcgcggtc cgattctcgg
     1981 tcgccgccag cgcgaggcga atctcgtcca ggcggtcgcg caggcttgcg gccttctcgt
     2041 agttcaagtc gcgcgcctcg cgcagcatct cggcctcgat ggcggcggcc agagcctccg
     2101 acggcaaccc cgcgtcgacg atcacgtcgg cgccgtcctt gcgcttggcg tcggcaatcg
     2161 cggtcgagag cgcgacttcg tccagcgatt tgatgatccc gcgcggcgtg atgccgtgct
     2221 cggtgttgta cgccagctgg agtgcccgcc ggcgacgggt ctcgtcgatg gcgcgccgca
     2281 tcgagtcggt gacctggtcg gcgtagaaga tcacgcgccc cgacacgtgg cgcgcggcgc
     2341 gccccgcggt ctgaatgagg ctacgctcgc tgcgcagaaa tccttccttg tcggcgtcgc
     2401 acacggcgac cagggagacc tccgggaggt cgagaccctc gcgcagcaga ttgatgccca
     2461 ccagggcgtc gaactcgccc aggcgcagtc cgcgcaggat ctccacgcgc tcgagcgcgt
     2521 cgatgtccga gtgcaggtag cgcaccctga tgccgagcga aagcagatag tccgtcagct
     2581 cctcggacat gcgcttggtc agcgtggtga cgagcacgcg ctcgccgttc tcggcgcacg
     2641 tgcgaatctc ttcgaccagg tcgtcgatct gaccctcgac ggggcgtacc acgatatcgg
     2701 ggtcgaccaa gcccgtgggc cgaatcacct gctcgaccac ttcgcccctc gtgcgcgcga
     2761 gctcgaactc cgacggggtc gcactcacat ggatgaagcg cgggaccagc gcgtcgaact
     2821 cgtcgaacgt aagcgggcgg ttgtcgaggg cggatggcag tcggaagccg tgctcgacca
     2881 gcgtcacctt gcgcgagcgg tctccatggt acatcccgcc cacttgcggg agcgtcacgt
     2941 gcgactcgtc gacgacgcac aagaattcgc tcgggaagaa atcgagcagc gtcaggggtc
     3001 gctcgccggg tttgcgcccg gacaaatagc gggcgtagtt ctcgatgccg ctgcagtaac
     3061 cgacttcttc gagcatctcg atgtcgtaga gcgtgcggga ctccaggcgc tgggcctcga
     3121 ccagcttgcc cagctcgcgc agctcgacca cgcgcgtcga cagctcgtcg cgaatctcgc
     3181 gcgccgcccg cttgatcacg tcttctggcg tcacgaagtg cttggccgga aacaacgtga
     3241 gcgtctcgag cgtgcgaatg cgcttgccgg tcagcgggtc cgcgatcgag atcgattcca
     3301 cggtatcacc gaagaactcg atgcggatgg cctcttcttc gtacgcggga cgaatctcga
     3361 ccacgtcgcc gcgcacgcgg aaggtaccgc gggtgaaggc ggcgtcgttg cgggagtact
     3421 ggatgtcgat caggctgcgc agcagatcct cgcggtcgag ggagtcgccg gccgccatct
     3481 cgatggtcat cgcgcgcacc atctcgggcg agcccaggcc gtagatgcac gacacgctcg
     3541 acacgacgat gacgtccttg cgcgtcatga gcgagctggt cgcgcgtacg cgcaggcgat
     3601 cgatgtcgtc gttgatggag gcgtccttgg cgatgtaggt gttggtcgac ggcacgaagg
     3661 cctcgggctg gtagtaatcg tagtagctca cgaagtactc gaccgcgttg tggggaaaga
     3721 agcccttgaa ctcgccgtac agctgcgcgg ccagcgtctt gttgtgcgag atgacgaggg
     3781 tgggcatgcc cacctccgcg atgacgttgg caatggtgaa ggtcttgccg gatccggtca
     3841 cacccagcag gacctggtcg cgcgtgtgat cgcgcagacc ctgcacgagg gcgcggatgg
     3901 cttggggttg gtcgccggtg ggtcggtagt gcgagacgag gtcgaagccg ggggccaaat
     3961 cgatcacccc gctgctccga tctcgacgac cgcctcgacg gcgctctgtt cgacccggtc
     4021 ggcgcggcgc ttctcgcgca gccacatcca cacgaacaac acgaccagga gaaccagcag
     4081 cgggaggatc aggcggcgaa tgacgggcgg ggggccgagc ttctcttcct tgagcgcgga
     4141 ttcgatctcc cacgacgggc ccgttccgct cccgccgctg gagcggctcg ccaccatcga
     4201 ccacagcgcg ttgccgtcga gccccaggaa ctcggcgtag ttgcgcagga agcccttgag
     4261 gtaggtctcg ctcggaaacg cgccgaagtc gtcgcgttcc agcgagcgga tggtctgcac
     4321 cgaaatgcgc gtctcgcgat tggcctgctc gacggtgagg ttcttctcct cgcgcgccgc
     4381 gcgcagcatc tcgccgatcg tcttgccctc gttattcacg cgtcaccccc ttcttgcccc
     4441 atcgtcccac gccgtcaagc ggccggtcaa tgccccaaga gccccagcga ctcgaccagc
     4501 ttcgacaatt cggtctcgag ccgctcccgg cgcgcgcgtt cgcgctcgac cacctcgggc
     4561 ttggccttgg cgacaaagcc ctcgttggcc agcttgcgcg ccacctcgga gagctcggcg
     4621 tcgaccttct cccgctcgcg tgtcaggcgc gcgcgctcgg cctccacgtc gaccacgccc
     4681 gcgagcggga tgacgacctc gaggtcaccg atcggcgtag cggcacttcc cttctccttg
     4741 gccacgtcgg gcccgacctc cagggtcgcg actcctgcca ggcgcacgat gccgtcggtc
     4801 gcctcgcgca ccagcgatgc cttgggttcg tgggcgcgca cgcgcacggg gatcttgagc
     4861 cccggattca cgcggtaggc ggcgcgcacg ttgcgcaccg aggtgacgac gtcgcgcagc
     4921 accgtcatgc ggccttcggc ttcggcgtcg agggcgcgcc cgtcgccctg cggatacgcg
     4981 tgctccaaaa gcagccgtcc gttcgacgtc ggcaaggcac tcgcgatctc ttcggtgatg
     5041 tagggcatga gcgggtgcat gagcttgagc gattcgccga gcacgtggac catcaccgcg
     5101 agcaccgtgc gcttgtcgcc ctccccgtac agccggacct tcgcgagctc gagataccag
     5161 tcacagaaat cgtgccagaa gaagtcgtag acacgcgcgg caccctcgtt gaggcgccat
     5221 tcttcgaagc accggttggt atccgacgcg cagcgcgaga gcgcggagag aatccagcgg
     5281 tcctcccacg cgtactcggg cctgaacgcc agggtcgacg taaaatcgcc gttcgcattt
     5341 tccttgaaga gcggtttccc ttctttgtcg accgccccca ggatcaagcg cgacgcctgc
     5401 cacatcttgt ttccgaaatt gcgccccagc tccagcttgg cttcgtcgaa aaagacgtcc
     5461 ttgcccggcg gtgagagcat cgagagcgtg aatcgaaacg cgtccgtccc ccatttgtcg
     5521 atgatgtcga gcgggtcggg cgagttgccc agcgacttgc tcatcttgcg gccttgtccg
     5581 tcgcgcacga tgccggtcaa gaacacatga cggaacggta cctcgcgcat gaactcgagc
     5641 gaggccatga tcatgcgcgc gacccagaag aaaatgatct ccgagccggt gacgagcacc
     5701 gaggtcgggt gatagcgctt taaatcgggc gcgtcttcgt cgggccatcc caggggtgag
     5761 aacgtccaca accaggacga gaaccacgtg tccaacacat cttcgtcctg gcgaatcttc
     5821 gctccgccgc agccttcgca ccgggtcggg tcggtgcgcg agaccgtcag gtggtcgcaa
     5881 tcgtcgcagt accacaccgg gatcctgtgc ccccaccaaa gctgacgcga gatgcaccag
     5941 tcccgaatgt tggtcatcca cgacatgtag acgttgcgcc agcgctcggg ataaaacgtg
     6001 atggcctcgg tttcggccgc gcggaccgcg ggttcggcca gcggcgccat cttgacgaac
     6061 cactgcgtcg acaagagcgg ctcgatgacg gtgccgcagc ggtcgtgctt accgaccgcg
     6121 tggcggtgcg gctcgacctt gacgacgagc ccgtcgtgct cgagtcgctc gatcaccttc
     6181 gcgcgcgcct cgaagcgatc gagccccgcg aaatcggcgc ccgcctgcgc ggtcatcttg
     6241 cccgtacgat cgatgaccac cacggtcgcg aggttgtggc gcttgccgat cccgtagtcg
     6301 accgggtcgt gcgcgggtgt gaccttgacg aagcccgagc cgaactcctt ttgcacggcg
     6361 gcgtcggcga cgatcgggag ctcgcggccg atgatcggca gcgtgcaggt ctttccgacc
     6421 aggtgccgtt tggccgcgtc ctcggggctg accgcgacgc ccgtgtcccc cagcatcgtc
     6481 tccgggcgcg tggtcgcgac cacgacctcc ccgtgcggcg acgggtagcg aatccaatac
     6541 agcttcgact cgcgctcttc gtgttggact tcctcgtccg agatcgcggt ctgatcgacc
     6601 ggacaccagt tgacgatgta gtcgccgcgg tagat
//
LOCUS       DASPGK010000331         4441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1487051, whole genome shotgun sequence.
ACCESSION   DASPGK010000331 DASPGK010000000
VERSION     DASPGK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487051"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VEC56_09315"
     CDS             <1..511
                     /locus_tag="VEC56_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14390.1"
                     /translation="GIGFFTNDGKSVGAVELEPDASGLVQTVFRIVSLEDGSEVYRFT
                     SPPQAIDPSEGGPGEWTYIDRNDPARNVYAMDYSSGGSARKITHFTDGRLTDHEWSPD
                     LRRIAVVRHDDNGENLWVVNADGSAPKQITKFDGEDIVEFDWTLDSKRVVVRAGTQSR
                     DVVLISNLR"
     gene            complement(544..1425)
                     /locus_tag="VEC56_09320"
     CDS             complement(544..1425)
                     /locus_tag="VEC56_09320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14391.1"
                     /translation="MRLKPWLLVLAAIGLSSFATGRAAGRTMTSNPRVQSVRGFYAAL
                     VDEMSRAGYDEGERSLLAHPHDYYGRYLDQRSRDYVALTVMPRIADAISFFELEGRPK
                     LRIFDLGCGLGMQSLIFASLGATVVGVDVREESIQLCRKRKAYYERELGRELDVEFHC
                     CDFTRANVADFDPPFDALFSMSAFSYVKPLDRTARRVSQLLSADGRVFLYEENASFAL
                     DRWKRSETLPTPAEVERALAAEGFRTEVLRGAGSIPSGLWRVPGLNETMLVPANEVLR
                     RSLRLSFKYVLAMERRG"
     gene            1711..2580
                     /locus_tag="VEC56_09325"
     CDS             1711..2580
                     /locus_tag="VEC56_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14392.1"
                     /translation="MRVRYRSLVAILVSLAATPVQAATWYVHPGGGGDTTTIQGGVDL
                     AANGDSVLVAPGTYTGAGNVNVLVDSKSITIVSESTAYNTILDCQGSGQGFIFVNADG
                     SVLHGFTVKNGLGAQGGGIRLDNTDMTVRYNIVSGNTATLAGGGIFVFKGNSTIHNNT
                     IVDNWAPAGGGLALKGPITGQVYQNIICTSAGGGGIDCTTGPFNTVFTCNDIWGNVGG
                     DAICAVDGGSNISVDPLFCGIPGSGNVYIQQTSQCTATFSPCGQAIGALGILCTTTKT
                     QSVTWGTIKALYR"
     gene            complement(2619..>4441)
                     /locus_tag="VEC56_09330"
     CDS             complement(2619..>4441)
                     /locus_tag="VEC56_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14393.1"
                     /translation="LAGSQISPQPVMDGSGGVVIAWYDIRNLSHYDIYAQRLNSSGTP
                     QWAANGVALCTALGDQYSPVVASDGVAGAIVAWHDNRTEPAYDIYARRISSAGAPLWQ
                     VDGVEISTGSGSQETPAIVSDGVGGAIIAWADSRSGNFDIYAQRVTGPGIPMWASNGV
                     AICSAFDDQSVLSIASDGASGAVVAWADHRFVQSDVYARRVNANGLPIWTFDGSPICS
                     AANNQDVAMVVSDGAGGGIIGWVDGRSWAQIYAQRAEPRYGTWGRPEPTIDSADDNPG
                     DEGGQVIVRWISSEHDRFYYPGISHYSVWRATDFVAAQASVTSPRMIESPAEVSRDFT
                     GAAIWEEPTANGPVFWEWVANQNAFYQDTYSVLAPTRQDEVPGVPAPHYFKVIAHEAN
                     FPQTRTWESAVVSAYSLDNLAPPAPRDLVGAPTLWDIPLSWSIDATPPDFSHYAVYRG
                     SAVVAPLPQYLIGTSLQTHHVDNSPPGGILRYIVTAIDVHGNESAPSNEWSPSGATPV
                     GDAPSIAALAVLDNVPNPFNGTTTLRIGLPKASGVEIEVFDVGGRRVRGEHAGILAAG
                     WREFAFDARDEDGGALPSGVYFYRVRAAGETVTRKMVIAR"
BASE COUNT          798 a         1445 c         1477 g          721 t
ORIGIN      
        1 ggggatcggt ttcttcacca acgatggtaa gagcgtgggt gccgtcgagc tcgagcccga
       61 cgcgagcgga ctcgtgcaga ccgttttccg catcgtctcc ctggaggacg gcagcgaggt
      121 ctatcgcttc acgtcgccgc cgcaggccat cgatccgtcc gaaggcgggc cgggcgagtg
      181 gacctacatc gaccgcaacg accccgcgcg caacgtctac gcgatggact actcgtcggg
      241 cgggtccgcg cgcaagatca cgcacttcac cgacgggcgc ttgaccgacc acgaatggtc
      301 gccggatctg cgacgcatcg cggtcgtgcg tcacgacgac aacggggaaa acctgtgggt
      361 cgtgaacgcc gacggcagtg cccccaagca gatcaccaag tttgacggcg aggacatcgt
      421 cgagttcgac tggacgctcg acagcaagag ggtcgtcgtc cgcgcgggaa cgcagagccg
      481 cgacgtcgtc ctgatcagca acctccggta gcctgccgcg cgatcaccag cgtgcacgcg
      541 tgcctagccg cgccgttcca tcgccaggac gtatttgaaa gacaggcgaa gacttctcct
      601 aagcacttca ttggcgggaa cgagcatggt ctcgttcagc ccggggacgc gccacaggcc
      661 gctgggaatc gagcccgctc cgcgcaggac ctcggtccgg aacccctcgg cggcgagggc
      721 ccgctcgacc tcggccggcg ttgggagcgt ctccgatcgc ttccagcgat ccagcgcgaa
      781 ggaggcgttc tcctcgtaga ggaatacgcg gccgtccgcc gagagcagct gcgatacgcg
      841 gcgcgcggtt cgatcgagcg gcttcacata ggagaaggcc gacatgctga acaacgcgtc
      901 gaacggggga tcgaagtctg cgacgttggc gcgggtaaag tcgcaacaat ggaactcgac
      961 gtccagctcg cggcccagct cgcgttcgta gtacgccttg cgcttgcggc acaactggat
     1021 ggactcctcg cggacgtcga cgccgaccac ggtggcgccg agactcgcga agatgaggga
     1081 ctgcatcccc aagccgcacc ccaagtcgaa gatgcgcagc ttcggtcgac cctccagctc
     1141 gaagaacgaa atggcatcgg cgatgcgggg catgaccgtc agcgccacgt aatcgcggga
     1201 tcgctgatcg agatagcgtc cgtagtagtc gtgcgggtgc gcgaggagcg agcgctcgcc
     1261 ctcgtcgtac cccgcccgcg acatctcgtc gaccaaggcc gcgtagaacc cgcggacgga
     1321 ctggacgcgc gggttggagg tcatcgtccg gccggccgcg cggccggtgg caaatgacga
     1381 gagtccaatg gccgcgagga cgaggagcca gggtttcaaa cgcatgcttc agcctctcct
     1441 ggaaggatgg aactcaacgg tcaccgtcaa ttctagcgga gtcctcggtg ctcgggcaac
     1501 ggctgttcga ggagtccgtc acgatggcgg atcacgagtc ggattgttca gcacgtctct
     1561 cccttcccgg gaatccacgg aaacgacgcg ccaccgcgaa gaaccgcccg cgcacaggcg
     1621 cgcgatggaa cacggattgc tcctatctat tattgaggtg ccgcgctccg attgggaagc
     1681 gcgcgcccgg gctgtccgaa ggagcgtcct atgcgagtcc gttaccgttc gctcgttgca
     1741 atcctcgtat ccttggccgc gactcccgtc caggccgcaa cgtggtacgt ccaccccggc
     1801 ggtggcggcg acaccaccac catccagggc ggtgtggacc tcgccgcgaa cggcgactct
     1861 gtgctcgtcg cgcctgggac ctacaccggc gccggcaacg tcaacgtgct cgtggacagc
     1921 aagtcgatca ccatcgtgtc cgagtccacg gcgtacaaca ccatcctcga ctgccagggc
     1981 agcgggcagg gattcatctt cgtaaacgcg gacggctcgg tcctgcacgg cttcacggtc
     2041 aagaacgggc tcggggcgca gggtgggggg atccgcctcg acaacaccga catgacggtt
     2101 cggtacaaca tcgtgagcgg caacaccgcg accctcgcgg gcggcggcat cttcgtgttc
     2161 aaggggaact cgaccatcca caacaacacg atcgtggaca actgggctcc ggcgggcggc
     2221 gggctcgcac tcaaggggcc gatcacgggg caggtctacc agaacatcat ctgcacgagt
     2281 gcgggcggcg gcggcatcga ctgtacgacg ggaccgttca ataccgtatt cacgtgcaac
     2341 gacatctggg gcaacgtggg tggggacgcg atctgcgcgg tcgacggtgg gagcaacatc
     2401 tcggtcgacc cgctcttctg cggtattccg ggtagcggaa acgtctacat ccagcagacg
     2461 tcgcagtgca ccgcgacgtt ctcgccctgc gggcaggcca tcggggcgct cggaatactg
     2521 tgcacgacga cgaagaccca gtccgtgacg tggggcacga tcaaggcgct ctaccgctag
     2581 agcgccgcgt tgccgccaca cgggcgacgc gcgaacgcct accgggcgat caccattttg
     2641 cgcgtgaccg tctcgccggc ggctcgcacg cgatagaagt agacaccact cgggagcgcg
     2701 ccgccgtcct cgtcgcgggc gtcgaaagcg aactctcgcc agccggccgc caatatgccg
     2761 gcgtgttcac cgcgaacgcg ccgtcccccc acatcgaaga cctcgatctc cacgccgctc
     2821 gccttcggca agccgatgcg caacgtggtg gtcccgttga agggattggg aacgttgtcg
     2881 agcacggcga gtgccgcgat ggaaggcgcg tctcccaccg gtgtcgcacc ggatggcgac
     2941 cactcgttgg acggcgcgct ttcgtttccg tgcacgtcga tggcggtcac gatgtaacgc
     3001 aggatcccgc ccggcgggct gttgtcgacg tggtgggttt gcaacgacgt tccgatcagg
     3061 tactggggta agggcgcaac taccgcggac ccgcgataca cggcgtagtg gctgaagtcg
     3121 ggtggtgtcg cgtcgatgct ccacgaaagg ggaatgtccc acaaggtcgg cgcgccgacg
     3181 agatcgcgcg gagcgggcgg cgcgaggttg tcgagggaat acgcgctcac gacggcggac
     3241 tcccacgtgc gcgtctgcgg gaagttcgct tcgtgcgcga tcaccttgaa gtagtgcggc
     3301 gcgggcacac cggggacttc gtcttgtcgg gtgggcgcga ggacgctgta ggtgtcctgg
     3361 tagaacgcat tctgattggc gacccactcc cagaacacgg ggccgttcgc cgtcggctct
     3421 tcccagatcg cggcgcccgt gaagtctcgg gagacctccg cgggactctc gatcatgcgc
     3481 ggcgacgtca cggacgcttg cgcagcgacg aagtcggtcg cgcgccacac cgaatagtgc
     3541 gagatgcccg gatagtagaa gcgatcgtgt tcgctcgaaa tccaccgaac gatcacctga
     3601 cccccttcgt cgcccgggtt gtcatcggcc gaatcgatgg tcggctcggg gcgtccccag
     3661 gtgccgtagc gcggctcggc gcgttgcgcg tagatctgcg cccagcttcg gccgtcgacc
     3721 cagccgatga tcccgccgcc ggcaccgtcc gagacaacca tcgcgacatc ttgattgttg
     3781 gcggcgctac aaatgggcga gccgtcgaag gtccatatcg gcaggccgtt cgcgttgacg
     3841 cgacgcgcat agacgtcgct ttgtacgaac cgatggtcgg cccacgcgac gacggctccc
     3901 gaagccccgt cggaagcgat cgacagcacc gattgatcgt cgaacgccga acaaatggcc
     3961 acgccgttgg acgcccacat cgggatgccc gggcccgtca cacgctgggc atagatgtcg
     4021 aagttgccgc ttcggctgtc cgcccacgcg atgatggctc cccccacgcc gtcggacacg
     4081 atggcggggg tttcctgcga acccgagccg gtgctgattt cgacgccatc gacttgccat
     4141 agcggtgcgc cggccgagct gatccggcgc gcgtagatgt cgtaggcggg ctcggtgcga
     4201 ttgtcgtgcc acgcgacgat cgcgcccgcc accccgtcgg aagcgacgac ggggctgtat
     4261 tggtcgccga gggcggtgca gagcgccacc ccgttggccg cccattgggg tgttcccgac
     4321 gaattgagtc gctgtgcgta gatgtcgtag tgactcaagt tccggatgtc gtaccaggcg
     4381 atgaccacgc cgccgctgcc atccatgacg ggctgcgggg aaatctggct cccggccaag
     4441 a
//
LOCUS       DASPGK010000332         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_551220, whole genome shotgun sequence.
ACCESSION   DASPGK010000332 DASPGK010000000
VERSION     DASPGK010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551220"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VEC56_09335"
     CDS             complement(<1..350)
                     /locus_tag="VEC56_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXV14394.1"
                     /translation="MKAIIPAAGIGTRLRPHTYTLPKALLYVAGKPIISHILDDVVPL
                     GPSSIVLIVGYKGELIEQFVRKKYPQVTVDFVFQEERRGIGHAVDLTRKVADTGEPLL
                     IVLGDTIIKTDLKKV"
     gene            complement(347..1393)
                     /locus_tag="VEC56_09340"
     CDS             complement(347..1393)
                     /locus_tag="VEC56_09340"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HXV14395.1"
                     /translation="MQALRERFDATDMLGRILAFPDQMEAAWKVGAAFARAVEPLAAK
                     EFERVVVAGMGGSAIGGDLAKSFLSDRAASPIVSCRDYALPKNVARGALFVASSYSGN
                     TGETLAAYDAARGVASAMVAVTSGGELAARCARDKVPCCIIPGGMPPRAAIGFSLLPM
                     LHLLRAAGAARFDDSEYHEALSAARATCADYAPSREDNRAVALARDLHGKTAFVYAAP
                     SLLEGVARRWSCQFNENAKTLAHFAFFPELNHNEIVGWEVGDLMAKAVILSLEDREDH
                     PMTRRHTEVGLSLMKPFAGKIERFEAPSGGRLSRMLATMLLGDFASVYLAYLNGVDPT
                     PVRKIDALKKEMSR"
     gene            complement(1396..3159)
                     /gene="ptsP"
                     /locus_tag="VEC56_09345"
     CDS             complement(1396..3159)
                     /gene="ptsP"
                     /locus_tag="VEC56_09345"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXV14396.1"
                     /translation="MAAKPEYERRGISAAPGIVIGPAFVRDAERLHVPQKLVEPPAVE
                     GEVARFLQALTDTARELRAFKSDIARRMGDDHARIFDAHLLILEDRMLVDETAAVIRN
                     ERLNAAAAFEKVIGKVIASIAGIEDEYLKERTIDVQDVRKRIIRNLIGKDTRAANRPS
                     EPSVLVAHDLSPSDTAQLDRRIVLGYVTDLGGRTSHTAILARSQGLPAVVGLENFVGR
                     VETGDTVIVDGNAGSVIVNPTPTTIEQYENEIARFRELEEQLLTLTGYPAHTLDGRSF
                     TLSANLDSSDEIDSVIEHGGQGVGLFRTEYFFISQEYLPSEEDQYKVYRQVAERMEGK
                     PVVIRTLDIGGDKIASYLNRSPELNPFMGWRGIRFCLTRKDIFKTQLRAIYRASAHGQ
                     IKIMFPMISQIEEVLRAKEICAEVREDLKRERYKFDESVPLGIMVETPSAVALADQYA
                     REVDFFSIGTNDLIQYTMAVDRGNSKIAHLYQHLQPSILRLLRLTVEAARKRKIHVSV
                     CGEMAGDPLSVPILIGLGIDEFSVSPNMIPEIKRIIRSVTFDECRALLRRVARFRTTN
                     EIETEIDAFLKSHAHNGRNGS"
BASE COUNT          525 a         1037 c         1079 g          595 t
ORIGIN      
        1 accttcttca agtcggtctt gatgatggtg tcgcccagga cgatcaagag cggctcgccg
       61 gtgtcggcga ccttgcgggt caggtccacc gcgtggccga tcccccgccg ctcctcctgg
      121 aacacgaagt cgaccgtcac ctgcgggtac ttcttgcgca cgaactgctc gatcagctcg
      181 cccttgtagc ccacgatcag cacgatgctg ctgggaccca gcggaaccac gtcgtcgagg
      241 atgtgggaga tgatgggttt ccccgccacg tacaggagtg ccttgggcag cgtgtaggtg
      301 tggggcctga gccgcgtgcc gataccggcg gccggaatga tcgctttcaa cgagacatct
      361 cctttttcaa cgcgtcgatc ttccttacgg gagtcgggtc gacgccgttg aggtacgcga
      421 ggtacacgct ggcgaagtcg ccgagcagca tggtggcgag catgcgggac aagcgcccgc
      481 ccgaaggggc ttcgaaacgc tcgatcttgc ctgcaaacgg tttcatgagc gaaagcccca
      541 cctcggtgtg gcggcgcgtc atgggatggt cctcgcggtc ctcgagcgag aggatgaccg
      601 cctttgccat caggtcgccc acttcccagc cgacgatctc attgtgattc agctcgggaa
      661 agaacgcgaa gtgcgcgagc gtcttcgcgt tctcgttgaa ctggcacgac caccggcgcg
      721 caactccttc taacaacgac ggtgctgcgt acacaaacgc cgtcttgccg tggaggtcgc
      781 gcgctagcgc gacggcgcgg ttgtcctcgc gcgacggcgc gtagtcggcg caggtcgcgc
      841 gcgcggcgga gagcgcttcg tggtactccg aatcgtcgaa gcgcgcggct cccgccgcgc
      901 gtagcaggtg cagcatcggc aagagcgaga acccgatcgc cgcccgcggc ggcatgccac
      961 cggggatgat acagcacggg accttgtcgc gcgcgcagcg cgcggccagc tcgccgccgc
     1021 tcgtcaccgc gaccatcgcg gaggccaccc cgcgcgcggc gtcgtaggcc gcgagcgttt
     1081 cgcccgtgtt tccggaatag ctcgacgcga cgaacagtgc cccgcgcgcg acgttcttcg
     1141 ggagcgcgta gtcgcggcag ctcacgatgg gagacgccgc tctatcggac aagaacgact
     1201 tcgcgaggtc gccgccgatg gcggagccgc ccatgcccgc gaccaccacc cgctcgaatt
     1261 ccttcgcggc cagcggctcg acggcgcgcg cgaacgcggc ccccaccttc caggccgctt
     1321 ccatctggtc ggggaacgcg aggatgcgcc cgagcatgtc ggtggcgtcg aagcgctctc
     1381 gtaaagcctg catggtcacg agccattcct tccgttatgg gcgtgcgatt tcaagaacgc
     1441 gtcgatctcg gtttcgatct cgttggtggt gcggaaacgc gcgaccctcc gcaggagcgc
     1501 tcggcactcg tcgaaggtta cggaacgaat gatgcgcttg atctcgggaa tcatattggg
     1561 actcacactg aactcgtcga tcccgaggcc gatcagaatc ggcaccgaga ggggatcgcc
     1621 cgccatctcg ccgcacacgc tcacgtggat cttgcgcttg cgggccgcct cgacggtaag
     1681 tcgcagcagg cggaggatgc tcggctgcaa gtgctgatac aaatgcgcga tcttgctgtt
     1741 cccgcggtcc accgccatcg tgtactggat caggtcgttg gtgccgatcg agaagaagtc
     1801 cacctcgcgc gcgtactggt cggccagcgc caccgcggac ggtgtttcca ccatgatgcc
     1861 cagcgggacg ctttcgtcga acttgtagcg ctcgcgcttc aagtcctcgc gcacctcggc
     1921 gcagatttcc ttggcgcgca gcacctcttc gatctgcgag atcatgggga acatgatctt
     1981 gatctggccg tgcgcgctgg cgcggtagat cgcccgcagc tgggtcttga agatgtcttt
     2041 tcgcgtcaaa cagaaacgaa tgccgcgcca gcccatgaac gggttcaatt cgggcgaacg
     2101 attgagatag ctcgcgatct tgtccccccc gatgtcgagg gtacgaatga cgacgggctt
     2161 gccctccatg cgctcggcga cctggcggta gaccttgtac tggtcctcct cgctcggcag
     2221 gtactcctgc gagatgaaga agtactcggt gcgaaacagc cccacgccct gccccccgtg
     2281 ctcgatcacc gagtcgattt cgtcggacga gtccagattc gcgctcagcg tgaacgagcg
     2341 gccgtcgagc gtgtgggccg gatagccggt gagggtcagg agctgctctt cgagctcacg
     2401 gaagcgcgcg atctcgttct cgtactgctc gatcgtggtg ggcgtgggat tgacgatgac
     2461 gctgcccgcg ttgccgtcga cgatgacggt gtcgccggtc tccacgcggc ccacgaagtt
     2521 ctcgagcccc accaccgcgg gcagcccctg cgagcgcgcg aggatggcgg tgtgcgaggt
     2581 acgcccgccc aggtcggtga cgtaaccgag cacgatgcgg cggtcgagtt gggcggtgtc
     2641 gctcggcgag aggtcgtggg caaccagaac actgggttcc gaggggcggt tggcggcgcg
     2701 ggtatccttg ccgatcagat tgcgaatgat ccgcttgcgc acgtcctgca cgtcgatggt
     2761 gcgctccttg aggtactcgt cttcgatgcc agcgatcgac gcgatcacct tgccgatcac
     2821 cttctcgaac gcggcggccg cgtttaggcg ctcgttgcga atcacggccg cggtttcgtc
     2881 gaccagcatg cggtcttcga ggatcaagag gtgcgcgtcg aagatgcggg cgtggtcgtc
     2941 gcccatgcgg cgcgcgatgt cggacttgaa cgcgcgcagc tcgcgcgcgg tgtcggtgag
     3001 ggcctgcaga aagcgcgcga cctcgccttc gacggcgggc ggctcgacca gcttttgggg
     3061 gacgtgcagg cgctcggcgt cgcggacgaa cgccgggccg atcacgatcc ccggtgccgc
     3121 cgagatgccc cggcgctcgt actcgggttt cgcagccatg gtggcgtcgg tcctattcct
     3181 cgtagaattt ggcgtcgaac agggcctcga gggctttgcg tgcggcggct tcgtcc
//
LOCUS       DASPGK010000333         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1369304, whole genome shotgun sequence.
ACCESSION   DASPGK010000333 DASPGK010000000
VERSION     DASPGK010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369304"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VEC56_09350"
     CDS             <1..962
                     /locus_tag="VEC56_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14397.1"
                     /translation="GAPDPTFTGTVDVQVREPSARRVYGTRCTSPAFMTYHIPGGTMY
                     DGTADVTNGEFEVSFRVPRYAATGTLAAASAYANQGGDDAGATIDSVLVVVPPSLADT
                     LALRPIDGAPRVDLGFKSGLKVVKPGDTVRAVVRDQDGINILSTTNEGKQAMLIDKLP
                     LPIDVNEFFQFDHGGIDTSGVLLFPLPDLTPGPHRLVYKVSDSFGSTTLDTLVFDVTD
                     AQNYYAEAVLNYPNPFQTSTQLLFRLSNRASIELEIYTVSGKRVRTIEDVREGGEAWI
                     EWDGRDAAGGELANGTYLYVATVDFVGIERAPVVLRGKMSKIR"
     gene            1025..2017
                     /locus_tag="VEC56_09355"
     CDS             1025..2017
                     /locus_tag="VEC56_09355"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HXV14398.1"
                     /translation="MMRKRLLGGALLAAALAAHGSAFAVGEAGVPSLTIPPGARPNGM
                     GEAFVAVSDDATAQWWNVGGLAFSKQRNLAFMHSQLVPDLASDVYYEFLGYSQRAGDV
                     GTWAISLIYLTYGESVATDPAGTPLGTFTSWEGTLSASFAMSLADNVGAGISMKFIRV
                     DYAPDEFTQDNAEGSGTSFAVDVGTLWKMPKQRLNVGFAVTNMGPNIAFIDREQSDPL
                     PITARLGTAWTAVSDDISNLLLTLDVEQSLVWLIDDEVDTRRSEIWHVGTEYRYVNLL
                     AGRLGYVYDDDGDFNAPTYGLGFIYKDKLSLDYANVPQAETLERVHRWSLYFSF"
     gene            2103..2990
                     /locus_tag="VEC56_09360"
     CDS             2103..2990
                     /locus_tag="VEC56_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14399.1"
                     /translation="MRSILTSIALVLCAGTSVDAARAGTGADAFPPGLSGYASRDAAV
                     RATRFASEEPAYYFQADPELAAASEPQSYWPVLYSALMPGVGELTMGYMGRGIALMAV
                     EVAAWTGYFVKHDDGIEGRDEYEAFADQYWAQRKFIDDHPLVYPQTGQTQEYLEEQGQ
                     AVSGSGGPWWTGYVPWVSKEEDKQHYYENIGKYDWFVSGWSDYDPTLNPPLAESALRD
                     EYVEMRNQSNDDLDDANKFVWVSLATRVFSIAETAIIVRNRREKSEQAGGGYDLPVAV
                     RARPRGFDGGELALEVRFK"
BASE COUNT          532 a          991 c         1005 g          490 t
ORIGIN      
        1 acggcgcccc cgaccccacc ttcaccggta cggtcgacgt tcaggtgcgc gagccgtccg
       61 cgcggcgcgt ctacggcacg cgctgcacgt cgcccgcgtt catgacgtat cacattccgg
      121 gtggcacgat gtacgacggg accgcggacg tgaccaacgg ggagttcgaa gtgagcttcc
      181 gcgttccgcg ctacgcggcg acggggacgc tggcggccgc gagcgcgtac gccaaccagg
      241 gaggcgacga cgccggcgcc accatcgaca gcgtgctggt cgtggtgccg ccctcgctgg
      301 ccgacacgct ggcgctgcgc cccatcgacg gcgcgccgcg cgtggacctg ggcttcaaga
      361 gcggtctgaa ggtggtcaag ccgggcgaca cggtgcgcgc ggtggtgcgc gaccaggacg
      421 ggatcaacat tctgtcgacc accaacgagg gcaagcaggc gatgctgatc gacaagctcc
      481 cgctcccgat cgacgtcaac gagttcttcc aattcgacca cggcggaatc gatacctcgg
      541 gcgtgctcct gtttccgctg ccggacctca cgcccggccc gcaccggctg gtgtacaagg
      601 tcagcgacag cttcgggtcg acgacactcg acacgctggt cttcgacgtg accgacgcgc
      661 agaactacta cgccgaggcg gtgttgaact acccgaaccc cttccaaacc tcgacccaac
      721 tcctgttccg actttccaac cgggcctcga tcgagctgga gatctacacg gtgtcgggca
      781 agcgggtacg cacgatcgag gacgtccgcg aaggcggaga agcgtggatc gagtgggacg
      841 gtcgcgacgc ggccggcggc gaactcgcca acggcaccta cctctacgtc gcgacggtgg
      901 acttcgtggg aatcgagcgc gcgccggtgg tcctgcgcgg caagatgtcc aagatacggt
      961 agtcgggcgc cttgacccgg aacccctgat agcattagta ttcttagcca gttaggagga
     1021 tttcatgatg cgtaaacggt tactgggtgg cgcgttgctg gcggcggcgt tggcagccca
     1081 cgggagtgca ttcgcagtcg gagaagcggg cgtgccgtcg ctcaccatcc cccccggcgc
     1141 acgtcccaac ggaatgggcg aggccttcgt cgccgtgtcc gacgacgcca ccgcccagtg
     1201 gtggaacgtg ggcgggctcg cgttctcgaa gcagcgaaac ctggctttca tgcacagcca
     1261 gctggtcccc gacctggcct ccgacgtgta ttacgagttc ctgggctact cccagcgcgc
     1321 cggcgacgtg ggcacctggg ccatcagttt gatttatctc acctacggcg agagcgtcgc
     1381 caccgacccc gccggcaccc cgctcggcac gtttacgagc tgggaaggca cgctcagcgc
     1441 gtcgttcgcg atgagcctcg cggacaacgt cggcgccggc atctcgatga agttcattcg
     1501 cgtcgactac gcgcccgacg agttcaccca ggacaacgcc gaaggctcgg gtacgagctt
     1561 cgcggtcgac gtcggcacgc tgtggaaaat gcccaagcag cgactcaacg tcgggttcgc
     1621 ggtgaccaac atgggcccga acatcgcgtt catcgaccgc gagcagagcg acccgctccc
     1681 gatcacggcc cgactgggca cggcgtggac cgcggtcagc gacgacatca gcaaccttct
     1741 gctcaccctc gacgtggagc agagcttggt ctggttgatc gacgacgaag tcgacacgcg
     1801 tcgcagcgag atctggcacg tgggtacgga gtaccgctac gtgaacctgc tcgcgggccg
     1861 actgggctac gtgtacgacg acgacggcga tttcaatgcg cccacgtacg ggctcggttt
     1921 catttacaag gacaagttgt cgctcgacta tgcgaacgtt ccgcaggccg aaacgctgga
     1981 gcgcgtgcat cgctggtcgc tgtatttttc gttctaggtc ttgacccccc tgcgtcccct
     2041 gcgaacgggc gtgtcgcccg cgcggacgcg cgcgcgacgt gtcgccggag gattcaaatc
     2101 gcatgcgttc gattctgact agtatcgcgc tcgtgctgtg cgcgggcacg agtgtggatg
     2161 ccgcacgcgc cggtaccgga gccgacgcgt ttccgcccgg gctctccggg tacgcgagcc
     2221 gcgacgccgc ggtgcgcgca acgcgtttcg cgtccgagga acccgcctac tacttccagg
     2281 ccgaccccga gctcgcggcc gcgagcgagc cgcagagcta ctggccggtg ctgtactcgg
     2341 cgttgatgcc gggtgtgggc gagctcacga tggggtacat gggccgcggc atcgcgctca
     2401 tggccgtcga agtcgcggcc tggaccgggt acttcgtcaa gcatgacgac gggatcgagg
     2461 ggcgcgacga gtacgaggcc ttcgccgacc agtactgggc ccaacgcaag ttcatcgacg
     2521 accacccgct cgtgtacccg cagacggggc agacgcagga gtacctcgag gaacaggggc
     2581 aggcggtctc gggtagcggc ggcccgtggt ggaccggcta cgtgccctgg gtctccaagg
     2641 aagaggacaa acagcactac tacgagaaca tcggtaagta cgactggttc gtctccggat
     2701 ggagcgacta cgaccccacc ctcaacccgc ccctcgcgga atcggcgttg cgcgacgagt
     2761 acgtcgagat gcgcaaccaa agcaacgacg acctcgacga cgccaacaag ttcgtgtggg
     2821 tgagtctggc cacgcgcgtg ttttcgatcg cggagacggc aatcatcgtg cgcaaccgcc
     2881 gcgaaaagag cgagcaggcg ggcggcggct acgacctgcc ggtggcggta cgggcgcgac
     2941 cccgcggctt cgacggcggc gagctcgcgc tggaggttcg gttcaagtga ttcgcatcgt
     3001 caccctcgcg tgcgcatg
//
LOCUS       DASPGK010000334         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1605505, whole genome shotgun sequence.
ACCESSION   DASPGK010000334 DASPGK010000000
VERSION     DASPGK010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605505"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VEC56_09365"
     CDS             <1..664
                     /locus_tag="VEC56_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HXV14400.1"
                     /translation="SPYMFYFDAGDFQLIGSSPEVLVKLEKRKATLSPIAGTRPRGST
                     AAEDQALEEELLKDEKERAEHVMLVDLGRNDLGRVCDTGTVKPESLMHVEKYSHVMHI
                     VSTVTGTLSPPHDAFDLLRACFPAGTVTGAPKIRAMEIIASLEREQRGPYAGALGYFG
                     HQGDMDMCITIRTIVMAGGRFHVQAGAGIVADSDPAREYEETLSKARALISAVAIAEE
                     GF"
     gene            664..1254
                     /locus_tag="VEC56_09370"
     CDS             664..1254
                     /locus_tag="VEC56_09370"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXV14401.1"
                     /translation="MHLLIDNYDSFTFNVAQALGALGEEVRVVRNDAIDVARAEAMQP
                     TTLIVSPGPGRPDDAGASKALIAHFADRIPILGVCLGHQCIVEVFGGEVVRAGRVMHG
                     KVSSVYHDGRTIYDGLSNPFSATRYHSLIAAERSLTDLLEISAYTSEGEVMGVRLKGR
                     PVEGVQFHPESILTPEGSKLLANFLGLARNHARETA"
     gene            1257..2288
                     /gene="trpD"
                     /locus_tag="VEC56_09375"
     CDS             1257..2288
                     /gene="trpD"
                     /locus_tag="VEC56_09375"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXV14402.1"
                     /translation="MMSAVLERLLRREDLAYDDARALMRVIVSGELSEPQIAAVLIAL
                     RAKGETVDEIAGFVSVLRDKAIRVTPARDDLVDTCGTGGDGTYTFNISTAAAIVAAGA
                     GVGIAKHGNRALSSKCGSSDVLEALGVRAVAPERVADVIDRTGIGFMFAPAHHPALKH
                     AAGVRRDLGVRTVFNLLGPMANPAFVKRQLVGVFDPALTETMARVLASLGSEKVYVVH
                     GSDGSDEVTVAGETRVTVLDEGRVETFSFAPESVGIERASARALTGGSPEENAHTIVA
                     VLDGARGAKRDAVVLNAGFVIAVAGRAGSIAEGVAMAAASIDDGRAKDVLERLRAATR
                     EIADDRRET"
     gene            2285..>3043
                     /locus_tag="VEC56_09380"
     CDS             2285..>3043
                     /locus_tag="VEC56_09380"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXV14403.1"
                     /translation="MSFLEAIALEKRREVAAKRAALSFDALRGRAGDTEPARDFLAAV
                     SRRGAVIAELKARTPTIASFTQSGSLLELAATYATNGASAISIVADPTRFGTSYGDVG
                     RVRASVPLPVLAKDFVIDPYQIAEARASGADAVLLIVRLLQLDELQNLVGFARELGME
                     ALVETHAESEVLAALAAGTRVVGINNRDLDTMIVSLDTTRRLAHLIPDDIVLVSESGI
                     VTRDDVADLTSRGARAFLVGGSLLGSDDPGALLRD"
BASE COUNT          483 a         1016 c         1051 g          493 t
ORIGIN      
        1 gtcgccctac atgttctatt tcgacgcggg cgactttcaa ctgatcgggt cgtctcccga
       61 ggtcctggtc aagctcgaga agcgcaaggc cacgctctcg cccatcgcgg gcacgcgccc
      121 gcgcggaagt accgcggccg aggaccaggc cttggaagaa gagctgctca aggacgaaaa
      181 ggagcgcgcc gagcacgtca tgctggtcga cctcggccgc aacgacctgg gtcgcgtgtg
      241 cgacaccggc acggtgaagc ccgaatcgct gatgcacgtc gagaagtact cgcacgtgat
      301 gcatatcgtg tcgacggtga cgggcacgct ctcgcccccg cacgacgcct tcgacctcct
      361 gcgggcctgc ttccccgcgg gaaccgttac gggtgcgccc aagattcggg ccatggaaat
      421 catcgcatcg ctagagcgcg aacaacgtgg tccttacgcg ggcgcactcg ggtatttcgg
      481 ccaccaaggc gacatggaca tgtgcatcac cattcgcacc atcgtcatgg ccggcggccg
      541 cttccacgtg caggcgggcg cgggcatcgt cgcggactcg gatccggcgc gcgagtacga
      601 ggagacgctg agcaaggcgc gcgcgctgat cagcgcggtc gcgatcgcgg aggaggggtt
      661 ctagtgcacc tcttgatcga caattacgac tccttcacgt tcaacgtggc gcaggcgctg
      721 ggcgcgctgg gcgaggaagt ccgcgtggtg cgcaacgacg ccatagatgt cgcgcgcgcg
      781 gaagcgatgc agccgacgac tctcatcgtt tcaccgggcc cgggccgccc cgacgacgcg
      841 ggggcgtcca aagccctcat cgcccacttc gccgaccgca ttccgatctt gggcgtgtgc
      901 ctcggccacc agtgcatcgt cgaagtgttc ggcggcgagg tggtgcgcgc gggtcgcgtg
      961 atgcacggca aggtgtccag cgtctaccac gacggccgca cgatttatga cgggctgtcc
     1021 aaccccttca gcgccacccg ttaccactcc ctcatcgccg cggagcgaag tctcaccgac
     1081 ctcctcgaga tttccgcgta cacgtcggag ggtgaggtca tgggtgtgcg tctgaagggg
     1141 cggcccgtcg agggtgtaca gtttcatccc gagtcgatcc tcacgcccga agggtcgaag
     1201 ctcttggcca atttcttggg gcttgcgcga aatcacgcgc gcgagacggc atagacatga
     1261 tgtccgccgt cttggaaagg ttgctgcgtc gcgaagatct cgcttacgac gacgcgcgcg
     1321 ctctcatgcg ggtcatcgtc tccggggaac tatcggagcc gcaaattgcc gccgtgctga
     1381 ttgcactccg cgccaagggc gagaccgtgg acgagatcgc cggcttcgtc tcggtgctgc
     1441 gcgacaaggc gattcgcgtc acacccgcgc gcgacgacct cgtcgacacc tgcggtaccg
     1501 gcggcgacgg gacgtacacg ttcaacatct ccaccgcggc cgcgatcgtg gctgcgggcg
     1561 cgggcgtggg gatcgcgaag cacggcaacc gcgcgctctc ctcgaagtgc ggcagctcgg
     1621 acgttttgga agcgctgggc gtgcgtgcgg tcgctcccga gcgggtcgca gacgtgatcg
     1681 atcgcaccgg catcggattc atgttcgcgc ccgcgcacca tccggccctc aagcacgcgg
     1741 ctggtgtgcg ccgcgacctg ggcgtgcgta ccgtgttcaa cctgctcggg ccgatggcca
     1801 acccggcctt cgtcaaacgc cagttggtgg gtgtgttcga ccccgcgctc accgagacca
     1861 tggcgcgcgt actcgcgtcg ctggggtcgg agaaggtgta cgtcgtgcac ggttcggacg
     1921 gcagcgacga agtcacggtg gccggcgaaa cgcgcgtcac cgtactggac gagggccgcg
     1981 tggagacatt ctcgttcgcg ccggaatcgg tcggcatcga gcgcgcgagc gcacgcgcac
     2041 tgaccggcgg ttcacccgag gagaacgcgc acaccatcgt cgccgtcctc gatggcgcgc
     2101 gcggggcgaa gcgcgacgca gtggtgctca acgcgggctt cgtgatcgcg gtggccggac
     2161 gcgcgggctc gatcgccgag ggggtcgcga tggcagccgc gtccatcgac gacggccgtg
     2221 ccaaggacgt actcgagcgg ctgcgcgccg cgacgcgcga aatcgcggac gaccgaaggg
     2281 aaacatgagc ttcctggaag caattgcttt ggagaagcgg cgcgaggtcg cggccaagcg
     2341 cgcggcgctc tccttcgacg ccctgcgggg gcgcgcgggc gacaccgagc cggcgcgcga
     2401 ctttctcgcg gccgtgtcgc ggcgcggggc ggtgatcgcg gagctcaagg cgcgcactcc
     2461 gaccatcgcg tcgttcacgc agtcgggttc gctcttggag ctggcggcga cgtacgccac
     2521 caacggcgcc tccgcgatca gcatcgtcgc cgacccgacg cgcttcggga cgtcgtatgg
     2581 agacgtcggc cgcgtgcgcg cgtcggtgcc gctgcccgtt ctcgcgaagg acttcgtgat
     2641 cgatccctat caaatcgcgg aggcgcgcgc ctccggcgcc gacgcggtgc tcttgatcgt
     2701 gcgcctgctc cagctcgatg aattgcagaa cctggtcggg ttcgcgcgcg agctcggtat
     2761 ggaggcgctg gtggagacgc acgcggaatc ggaggtgctg gccgcgctcg cggcgggtac
     2821 gcgcgtcgtc ggcatcaaca accgcgacct ggataccatg atcgtgtcgc tcgataccac
     2881 gcggcgcctg gcccacctca tccccgacga catcgtgctg gtcagcgaga gcggcatcgt
     2941 cacccgcgac gacgtcgccg acctcacctc gcgcggcgcg cgcgcattcc tggtgggggg
     3001 gagcctgctc ggctccgacg atcccggcgc gctcttgcgc gac
//
LOCUS       DASPGK010000335        11219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_668674, whole genome shotgun sequence.
ACCESSION   DASPGK010000335 DASPGK010000000
VERSION     DASPGK010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668674"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VEC56_09385"
     CDS             <1..789
                     /locus_tag="VEC56_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXV14404.1"
                     /translation="RSPYHGFSVEFAEYRPYMPGDSIKTVDWKVYGKTDRTYVKVFEE
                     ETNLIANVLLDKSGSMGFPEGGTTKLEYGSMLAASLAYLMIRQQDAVGLCLFDDEVRA
                     RIPHRSVRKQLFHILQTLEGTAPGAKTSISPALHDLAERMGRRGLVVLISDLMDDPEK
                     VLMGLKHFRHRRHEVIVFHVLDPREMDVDYKDEVEFEDMETGQKLRVEPAFLREQFKK
                     DVRAWIDTLERGCRNHHIDYHLLRTDTPFDRALTAYLGKRARLG"
     gene            793..2871
                     /locus_tag="VEC56_09390"
     CDS             793..2871
                     /locus_tag="VEC56_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HXV14405.1"
                     /translation="MGFLNIAFLVGLVAAAVPIIIHLLNRRRVKRVKFSSLEFLDEVN
                     KQRMRRINMRRILILILRTLAILALVFAFARPTLRTGLLFAGSVPKNVVVCVDASYSM
                     GAAMETGTAFDAAKRIAGEIVDEAGGSDAINVIAFAKTPEALLEQGTRNKGVVKAAID
                     RVGLSSETTSIRGAIDRALDLMEKSDVEGGEVFVVSDFRASEDSAFVDEAALPANVRV
                     YFVPVYLEDVDNVSIDRVAVPRKLLRPGEVVKVSVTATNHAREANASVPLEIVVDGDR
                     KAEQMVELAPNATQTVTFPLSLAEPGRYHGRVAKNRDRLPVDDDRFFLIEVSNTIPVT
                     VVTGRRRATSADAPVPSVFYVEKALNPRGTSEGEFNVRVIDERDVTASALPGSGVVVW
                     VDPQSMEPKRMVLLERHVRRGGGLMVFLGNAAPAVLNDSAFRALVGIRGGAEKGAESR
                     AAFTSFEKGHPVFSLFTKDELELLARARVRAYTSARGVAPDSALAYVGGGDPAVWECV
                     RGRGRVLVFAAAPDLATGDIPLSPMFLPLVHTSVSYLASSGEAPGADEHVVGSPIAFT
                     VSQTGLDESQLAIRDPAGESLKPAIGERSAGEVQVGFDRPAHPGFYRLYRDTTHVAET
                     AVNVDTRESNLAVSALADKRPKSVSVVRASDSFRTDLREAKEGREVFAAFLLFAVAAL
                     VAESVLGRKA"
     gene            2868..6485
                     /gene="mfd"
                     /locus_tag="VEC56_09395"
     CDS             2868..6485
                     /gene="mfd"
                     /locus_tag="VEC56_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXV14406.1"
                     /translation="MIDNVRRLPQTNSLRAAIDEAARAAREVAQAEAGRAIDAPRGLH
                     GEKLAARCRVHVAGLKGSSTVLVAEAIRQSLERSLLLVYPDDEAAQDATSDFRTLSHG
                     SVVHFPERAVAPHRFELRENLAAGGDRNESLLKILNGAADVVVTSVLGFIEKTIPRRQ
                     LSSHQRVLSVGDTLDLDALREHLVDMGYDAVTVIEEAGQFAVRGAIVDVFDPAWDYPA
                     RVELDGDEIASIRSFDLETQRSIQALQTCTMLPAGSVPLDREAVAHLRAYLEARDFAP
                     ELIEKISDEAEHSRSSYVWRRYAPALGMTGSLLEFFDEPPIVWFVDGEGTNRVLANLA
                     RDFARNAQRAEDEFPPLALGDYVHPIDHVRAHGSAVVVHWALGDVTAAPAADSPKADA
                     VPPARDGETFVRFQTTAHPSVVGKLDPLLKQIKGLGAKGVDVMIYSETPTQRDRLADM
                     LGEDELLVHLPVGWIASGFIWEQAGLAILTDHQIFNRMLARPRKKRAKRRTTTVRTDS
                     LQGGDYVVHVDYGIGRFVGLEKISADDSATECLTVRFDGGDRIFVPLDQIHLVEKYIG
                     REGVVPKLDKLGGSRWQHTKERAKKAIEDVARDLLRVYAQRELATAPAFGPDTQWQHE
                     LEASFPYEETPHQISVTREIKDDLETAHPMERLVCGDVGFGKTEVAIRAAFKVASSGK
                     QVAVLVPTTLLAFQHQRTFTERMKQFPLRIAMLSRFVSTAEQKQTVAALREGTVDVVI
                     GTHRLLSKDVAFRDLGLLVVDEEHRFGVKHKERLKEIAKGVHVLSLTATPIPRTLYMS
                     LSGLRKISLIETPPRNRHPVKTEVTAFDEDVIARAISEEIGRDGQVFFVHNRVQSIHS
                     MKAFLEKLLPGVRFGVAHGQMKEDELENAVLDFMSRKYDVLISTMIIESGLDFPNANT
                     LIVSRADRLGLAQLYQLRGRVGRREQQAYAYFLVPRQISLTPQAVKRLQAMEEFEELG
                     SGYRLAMRDLEIRGAGNVLGTEQHGHVAAIGFELYTKMLKETVDQLRGEAHEEAPPCR
                     VEAPYSCYLPDRYVSDADERMLLYKRIARMADPDEAVALGDELRDRFGEPPPPAQMLI
                     DLAHIKLEASRLGVVMVHLRDPGHRLLARDGAARKEPEGVQSLPRGEASLEFAPGRAL
                     SPQAAARLSETFGRRILFKSGKTFAVSLIGQPPARLVDEVKNLLQVARFASKINSLPK
                     R"
     gene            6535..8271
                     /locus_tag="VEC56_09400"
     CDS             6535..8271
                     /locus_tag="VEC56_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXV14407.1"
                     /translation="MRIRMMWLGLAVLLGVAATGCSKREELKVAEFKDEVITVADFEQ
                     AYARVDPAYLPKATGQEGKLEFLNTMLNKYVMAWKADELGYDKDPTVTQGMEVFSRMA
                     LQVAYLKKQVADKITVSDKEVRQHYDNQGVSMECKQILCDTPDQAEEAYQAIQDGGDF
                     DAVLRQYSKNEDAATGGMVFTAVYGQLTPEMQEALFAVPVGGVTPPVMTAHGWVINKV
                     LRRNEGRAKPPYEDVKEEYTTQVKNLKEAVALNAFTNKLRDDYGVTWHYDNIGIVFNA
                     LPPDRDFDDAPARSTEIYPLLLFDPADLDKPMVTWQGGKQITIKDFSDFYDQASFFNR
                     PRRNTRYGGIRQFLTERIMNEISIDVVRKSGIENDPEVAKALKAKKEEIMVNLLYDDV
                     INKQTVVTHQEIVDYYEGNLEAFHTPERRRFGVILAGDIEAAQQAYKDLRAGQSFRTV
                     MLAFSVDEETKQNNGETKDLARGEQPEMDAVGFSLQRVGDFSEPFQTSRGWMVLKLVE
                     RIDEKYFSLEEAEGRVETALKTMKNDDRLKEMLTKWKEEVGVVIYEDNLAKTHIVERQ
                     PAANVLDPASLN"
     gene            8334..9422
                     /locus_tag="VEC56_09405"
     CDS             8334..9422
                     /locus_tag="VEC56_09405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV14408.1"
                     /translation="MPPIAFRGLRKAVITLVAAGAFAAPACERREVGAPSIDPGPVVA
                     RINGQPLYRTALDAYLPEDEFALSTIEERRTYFDRWVATQLLYEEAARVGMGVSDEVA
                     RKLEQYKKDMIADRLVQEVLDERAVVTRGEVMRYYRNHKDEFNLEVRVSHILTNTIEE
                     AQEAQEMLKTRPFSLVARKMSVDKHTGAGGDLGYLSKGNMLPEFEDVVFRMRRGEVSD
                     IVESEFGYHIIKLTDVRTSLNELPFEQVAPEISRTLLIRKRAAVYDSLVTALVEKARI
                     EVIDPDLKFAIERAESLRTARLAGEQAAREFRKALPEPGEGRASSASSSNGTRATAPP
                     DTAVVAPPEAAPAQEPESTEKADSSGGE"
     gene            9454..10731
                     /locus_tag="VEC56_09410"
     CDS             9454..10731
                     /locus_tag="VEC56_09410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV14409.1"
                     /translation="MLLVAAAMVPANAFAQTRIIDRIVAVVDDEAIFESDVEQAVRQA
                     LVQRGQTSIPTAERQQLFEEALAVLINDRLVIAQAARLGIDVPFSDVEAEVNKALDEN
                     RAALGGDDAFNAQLAAEGLTLEDLKKMYRVQIKNRMLVERVLRRDMDRTRAEPTEEEL
                     RALFESRKSELPERPEVAHLQTIFIGFETSSSASSATRAKIETLRARIEGGEDFGEVA
                     KKESEDPSAALGGDLGFLRPKDLREPAFANAVSSLKMGEVSQPVLTVYGYHLIQITDE
                     RKATGEVRVRHILVRAQATDTDIDEVFKTANFIHEEVKRGVPFDSLAARYNTDPAAGK
                     NGDLGWVRVTELPQFFQDVLKEMKPGDVSQVLRESSGFRIVKLIDRETARPYEYAEVR
                     DEVKRLYEQERFATNYENYLADLRKKFHVEIRS"
     gene            complement(10728..>11219)
                     /locus_tag="VEC56_09415"
     CDS             complement(10728..>11219)
                     /locus_tag="VEC56_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HXV14410.1"
                     /translation="ARFVRHVRSFWETPRHRMSTRVAEEINGLIARGELSIVAGRVAE
                     ATASATAISVTVRPRGSAMTERYNAGAVVNCTGPDGDFARLREPLVVAMRERGALVPD
                     SLGLGVVTDASGALVDAHGRASPVLYTLGSPRRADDWESTAVPELRVQAERLSHRLRE
                     SLP"
BASE COUNT         2107 a         3696 c         3632 g         1784 t
ORIGIN      
        1 cgcagcccct accacggctt ctcggtcgag ttcgccgagt atcgtcctta tatgcccggc
       61 gacagcatca agaccgtcga ctggaaggtc tacggcaaga ccgaccgcac ctacgtcaag
      121 gtcttcgagg aagaaaccaa cctcatcgcc aacgtcctct tggacaagag cgggtcgatg
      181 gggttccccg agggcggcac caccaagctc gagtacggct cgatgctggc cgcgagtctc
      241 gcgtacctca tgatccgcca gcaggacgcg gtgggcttgt gcctcttcga cgacgaggtg
      301 cgcgcgcgca tcccgcaccg gtcggtgcgc aagcaattgt tccatattct gcaaacgctc
      361 gagggcacgg cgccgggggc caagacctcg atcagccccg cgctccacga cctggccgaa
      421 cgcatgggcc ggcgcggtct ggtggtcttg atctcggatc tcatggacga ccccgagaag
      481 gtgctcatgg gcttgaagca cttccggcat cgccgccacg aagtgatcgt gttccatgtc
      541 ctcgacccgc gcgagatgga cgtcgactac aaggacgaag tggaattcga agacatggaa
      601 accgggcaga agctccgggt ggagcccgcg ttcctgcgcg agcaattcaa gaaagacgtg
      661 cgcgcgtgga tcgacaccct cgagcgcggg tgtcgcaacc accacatcga ctatcacctg
      721 cttcgcaccg acaccccctt cgaccgcgcg ctgacggcgt acctcggcaa gcgcgcgcgc
      781 ctgggataga ccgtgggctt tctcaatatt gcattcctgg tcgggctggt cgcggccgcg
      841 gtcccgatca tcatccattt gctgaaccgg cgccgcgtca agcgggtcaa gttctcgtcg
      901 ctcgagttcc tggacgaggt caacaagcag cgcatgcggc gcatcaacat gcggcgcatt
      961 ctcatcttga tcctgcgcac gctcgcgatc ttggccctcg tgttcgcgtt cgcgcgcccg
     1021 acgctgcgca cgggtctctt gttcgcgggc tcggtgccca agaacgtcgt ggtgtgcgtg
     1081 gacgcgagct acagcatggg cgccgcgatg gagaccggca ccgcgttcga cgcggccaag
     1141 cgcatcgcgg gcgagatcgt ggacgaagcg ggcgggagcg acgcgatcaa cgtgatcgcc
     1201 ttcgcgaaga cacccgaagc gctgctcgag caggggacgc gcaacaaggg cgtggtcaag
     1261 gccgccatcg atcgggtggg actctcgagc gagaccacgt ccatccgcgg cgccatcgac
     1321 cgcgcgctcg atctcatgga aaagagcgac gtcgaaggcg gcgaggtgtt cgtcgtctcg
     1381 gactttcgcg ccagcgagga ttcggccttc gtcgacgaag cggcgctgcc cgccaacgtg
     1441 cgggtgtact tcgtcccggt gtacctcgaa gacgtcgaca acgtcagcat cgaccgcgtc
     1501 gccgttccgc gcaagctcct gcgtcccggc gaagtggtca aggtctcggt gacggccacc
     1561 aaccacgcgc gcgaggcgaa cgccagcgtg ccgctcgaga tcgtcgtgga cggcgatcgc
     1621 aaggccgagc aaatggtcga attggcgccc aacgcgaccc aaaccgtgac gtttccgctt
     1681 tcgctcgccg agccggggcg ctaccacggg cgcgtggcca agaatcgcga ccgcctgccg
     1741 gtcgacgacg accgcttctt cctgatcgaa gtctccaaca cgattccggt caccgtggtg
     1801 accggtcgcc gccgcgcgac cagcgccgat gcaccggtgc cctcggtgtt ctacgtggag
     1861 aaggcgctca atccgagggg aacgtcggag ggcgagttca acgtgcgcgt catcgacgag
     1921 cgcgacgtca ccgcgtccgc gctcccggga agcggtgtcg tggtgtgggt cgacccgcag
     1981 agcatggagc ccaagcgcat ggttctcttg gagcgacacg tgcgacgcgg cggcgggctg
     2041 atggtgttcc tcggcaacgc ggcgcccgcg gttctcaacg acagtgcctt ccgcgcgctg
     2101 gtcgggattc gcggcggcgc ggagaagggg gccgagtcgc gcgccgcgtt cacatccttc
     2161 gagaaaggcc acccggtctt cagcctcttt accaaagacg agttggagtt gctcgcgcgc
     2221 gcacgcgtgc gcgcgtacac ctcggcgcgc ggtgtggcac cggactccgc gcttgcgtac
     2281 gtcggcggcg gcgaccccgc ggtgtgggag tgcgtgcgcg gccgcggtcg cgtgctcgtg
     2341 ttcgccgccg cacccgacct cgcgaccggc gacatcccgc tctctcccat gttcctcccg
     2401 ctcgtgcaca cctcggtgtc gtacctcgcg tcgtcgggcg aagcccccgg cgccgacgag
     2461 cacgtggtgg gctcgccgat cgcgttcacc gtgtcgcaga ccggcctcga cgaatcgcag
     2521 ctcgcgatcc gcgatcccgc cggcgaatcg ctcaagccgg cgatcggaga acgcagcgcg
     2581 ggcgaagtcc aagttggctt cgaccgcccc gcgcaccccg gattctatcg gctgtatcgc
     2641 gacaccacgc acgtcgccga gaccgccgtc aacgtcgaca cgcgcgagtc gaaccttgcc
     2701 gtctcggccc tcgcggacaa gcgacccaag tcggtgagtg tcgtgcgcgc aagcgattcc
     2761 ttccgcaccg accttcgcga agccaaagaa ggccgcgaag tcttcgccgc gtttctcctg
     2821 ttcgcggtgg cggcgctggt ggcggagtcg gtgctcggcc gcaaagcatg atcgataacg
     2881 tccggcgtct tcctcagacc aactctcttc gcgcggccat cgacgaggct gcccgcgcgg
     2941 cgcgcgaggt cgcgcaggcc gaagcgggcc gtgccatcga cgcgcctcgt ggtctccatg
     3001 gagagaagct cgcggcgcgg tgtcgcgtcc atgtcgcggg cctcaagggc tcttcaacgg
     3061 ttctcgttgc cgaggcgatt cgacaatcgc tggaacgaag cctcctcctc gtctatcccg
     3121 acgacgaagc cgcgcaggac gcgacgtcgg actttcgtac gctctcgcat ggcagcgtgg
     3181 tgcactttcc ggagcgcgcg gtggcaccgc atcgcttcga actgagggag aacctggccg
     3241 cgggcggcga tcgcaacgag agcttgctca aaatcttgaa cggcgcggcc gacgtggtcg
     3301 tcaccagtgt gctgggcttc atcgaaaaga cgatcccgcg ccggcagctt tcctcgcacc
     3361 agcgcgtgct ttcggtcggc gacacgctcg acctcgacgc gctgcgcgag cacctggtcg
     3421 acatgggcta cgacgccgtg accgtgatcg aagaggcggg gcagttcgcg gtgcgcggtg
     3481 ccatcgtcga cgtgttcgac ccggcctggg attaccccgc gcgcgtggag ttggacggcg
     3541 atgagatcgc gtcgatccgc tccttcgatc tcgaaacgca acgatctatt caagccttgc
     3601 agacgtgcac gatgttgccc gccggcagtg tgcccttgga ccgggaagcg gtcgcgcact
     3661 tgcgagcgta cctcgaagca cgggacttcg cgcccgagtt gatcgagaag atctcggacg
     3721 aagccgagca ctcgcgctcg tcgtacgtgt ggcgccgcta tgcgcccgcc ctcggcatga
     3781 cgggctcgtt gctggagttt ttcgacgagc cgcccatcgt gtggttcgtc gacggcgaag
     3841 gcaccaaccg cgtgctcgcc aacctggcgc gcgacttcgc gcgcaacgcg cagcgcgccg
     3901 aagacgagtt cccaccgctc gcactcggcg actacgtaca cccgatcgac cacgtgcgcg
     3961 cgcacgggtc agccgtcgtc gttcattggg ccttgggaga cgtcaccgcc gcgccagcgg
     4021 cggattcgcc gaaggccgat gcggttcctc cggcgcggga cggtgagaca ttcgtacggt
     4081 ttcagacgac ggcgcatccc agtgtcgtcg gaaaactcga tccgctcttg aagcagatca
     4141 agggtctggg cgccaagggc gtcgacgtga tgatctactc ggagacgccg acccagcgcg
     4201 accgcctggc cgacatgctg ggcgaagacg agttgctggt gcatctgccg gtgggatgga
     4261 tcgcgagcgg gttcatctgg gagcaggcgg gcctcgcgat tctcaccgac caccagatct
     4321 tcaaccgcat gctggcgcgc ccgcgcaaga agcgcgcgaa gcgccgcacc accacggtgc
     4381 gcaccgacag cttgcagggc ggcgactacg tggtccacgt cgactacggc atcgggcgct
     4441 tcgtgggcct ggagaagatc agcgccgacg acagcgctac cgagtgcctc accgtccgct
     4501 tcgacggcgg cgaccgcatc ttcgtcccgc tcgatcagat tcacctggtc gagaagtaca
     4561 tcggccgcga aggggtggtg ccgaagctcg acaaactggg tggctcgcgc tggcagcaca
     4621 ccaaggagcg cgccaagaaa gcgatcgaag acgtcgcccg cgatctcctt cgcgtgtacg
     4681 cgcagcgcga attggccacc gcacccgcgt tcgggcccga cacgcagtgg cagcacgaat
     4741 tggaggcgtc cttcccctac gaagagaccc cgcaccagat cagcgtcacg cgcgagatca
     4801 aggacgactt ggagaccgcg cacccgatgg agcgcctggt gtgcggcgac gtcggttttg
     4861 gcaagaccga ggtggcgatc cgcgccgcct tcaaggtggc ctcgagcggc aagcaggtcg
     4921 cggtactggt gccgaccacg ctgctcgcgt tccagcacca gcgcacgttc accgagcgca
     4981 tgaagcagtt cccgctccgc atcgcgatgc tctcgcgctt cgtctcgacg gcggagcaaa
     5041 agcaaaccgt ggcggcgctg cgcgagggga ccgtcgacgt cgtgatcggc acccatcgtc
     5101 tcctatcgaa agacgtcgcc ttccgggacc tcgggctctt ggtcgtcgac gaagagcatc
     5161 gcttcggcgt caagcacaag gagcgcctga aagagatcgc gaagggcgta cacgtgctct
     5221 cgctcacggc gacgccgatt ccgcgcacgt tgtacatgtc gctctcggga ctgcgaaaga
     5281 tctcgctcat cgaaacgccg ccgcgcaacc gacacccggt caagaccgag gtgaccgcct
     5341 tcgacgaaga cgtgatcgca cgggccatct cggaggagat cggccgcgac ggtcaggtgt
     5401 tcttcgtcca caaccgcgtg cagtcgattc actccatgaa ggcgtttctc gaaaagctgc
     5461 tgcccggcgt gcgcttcggc gtcgcgcacg ggcagatgaa ggaagacgag ttggagaacg
     5521 cggtgctgga tttcatgagc cgcaagtatg acgtgctgat ctcgaccatg atcatcgagt
     5581 cgggtctcga ttttcccaac gccaacacgc tgatcgtcag ccgcgcggat cgattgggcc
     5641 tcgcgcagct ctaccaattg cgcggacgcg tcgggcgccg cgagcaacag gcgtacgctt
     5701 actttctggt accgcggcag atttcgctca caccgcaagc ggtcaagcgc ctgcaagcga
     5761 tggaggagtt cgaggaattg ggaagcggct accgcctcgc catgcgcgac ttggagattc
     5821 gcggcgcggg caacgtgctc ggaaccgagc agcacgggca cgtcgcggcg atcggcttcg
     5881 agctgtacac caagatgctc aaggaaacgg tcgatcagtt gcgcggtgaa gcccacgaag
     5941 aggcaccccc gtgccgcgtc gaagcgccct acagttgtta tctccccgac cggtacgtgt
     6001 ccgacgccga cgaacgaatg ctgctataca aacgaatcgc tcgcatggcc gacccggacg
     6061 aagcggtggc actgggcgac gagttgcgcg accgcttcgg cgagccgccc ccgcccgcgc
     6121 agatgctgat cgacctcgcc catatcaaac tggaggcgag ccggctcggg gtcgtcatgg
     6181 tccatctgcg tgacccgggc catcgtttgc tggcccgcga cggcgctgca cgcaaggagc
     6241 ccgagggcgt gcaaagcctt ccccgcggcg aagctagcct ggaattcgcg cccggtcgcg
     6301 cgctttcacc ccaggccgcg gcccggctct cggaaacttt cgggcggcgt attctgttta
     6361 agtccgggaa gacattcgcg gtcagcctca tcggccagcc gcccgcgcgg ctcgtcgacg
     6421 aggtgaagaa cctgttgcaa gtagcccgct tcgccagtaa aatcaacagc ttgccaaagc
     6481 gataggggca acccgtcgct cctccatgca tcgtcaggaa aggagtttcg ttcgatgagg
     6541 attcgtatga tgtggctcgg gctcgccgtg ctcttgggtg tggctgccac cggttgctcg
     6601 aagcgtgaag agctcaaggt cgccgagttc aaagacgagg tgatcacggt cgccgatttc
     6661 gaacaggcgt acgcacgggt cgacccggcc tacctgccca aagccaccgg ccaggaaggc
     6721 aaattggaat tcctgaacac gatgctcaac aagtacgtca tggcgtggaa agccgacgag
     6781 ctgggatacg acaaggaccc caccgtgacg caaggcatgg aagtgttctc gcgcatggcg
     6841 ctgcaggtcg cctatctgaa aaagcaggtc gcggacaaga tcaccgtgtc cgacaaggaa
     6901 gtgcgccagc actacgacaa ccaaggtgtg tcgatggagt gcaagcagat cctgtgcgac
     6961 acccccgacc aggcggaaga agcctatcag gccattcaag acggcgggga cttcgacgcg
     7021 gtcctgcgcc aatactcgaa gaacgaggac gcggccaccg gcggcatggt gttcaccgcc
     7081 gtctacggac agctgactcc cgagatgcag gaggcgttgt tcgcggttcc ggtcggaggg
     7141 gttacgccac cggtcatgac cgcgcacggt tgggtcatca ataaggtcct gcgtcgcaac
     7201 gaaggtcgcg cgaagccgcc ctacgaggac gtcaaggagg agtacacgac ccaggtcaag
     7261 aacctcaagg aagccgtcgc gctcaacgcg ttcaccaaca agctccgcga cgactacggc
     7321 gtcacctggc actacgacaa tatcgggatc gtgttcaacg cgctgccgcc cgatcgcgat
     7381 ttcgacgacg cgcccgctcg cagcacggag atctatccgt tgctcctgtt cgatccggcc
     7441 gacctcgaca agccgatggt gacgtggcag ggcggcaaac agatcacaat caaggatttc
     7501 tcggacttct acgaccaggc gtccttcttc aaccgcccgc gtcgcaatac ccgctacggc
     7561 ggcatccggc agttcctgac cgagcgcatc atgaacgaga tctccatcga cgtggttcgc
     7621 aagtccggca tcgaaaacga ccccgaggtt gcgaaggcgc tcaaggccaa gaaagaagag
     7681 atcatggtca atctcttgta cgacgacgtg atcaacaagc agaccgtggt cacgcaccaa
     7741 gagatcgtgg actactacga aggcaacctc gaggctttcc atactcccga gcggcgccgg
     7801 tttggcgtca tcttggcggg cgacatcgag gcggcgcagc aggcctacaa ggacctgcgc
     7861 gccggacagt ctttccgcac ggtcatgctc gccttttcgg tcgacgagga gaccaagcag
     7921 aacaacgggg agacgaagga tctcgcgcgc ggcgaacaac ccgagatgga cgcggtcggg
     7981 ttctcgctgc aacgcgtggg cgacttctcg gaaccgttcc agacctcgcg cgggtggatg
     8041 gttctcaagc tggtcgagcg tatcgacgag aagtatttct cgctcgagga ggcggaagga
     8101 cgcgtcgaaa ccgcgctcaa aacgatgaag aacgacgatc gcctgaagga aatgctcacg
     8161 aagtggaagg aagaggtcgg cgtcgtcatc tacgaggaca acctggccaa gacgcacatc
     8221 gttgagcgtc agccggccgc caacgtgctc gacccggcta gcctcaacta gccgcgttca
     8281 gcgtggagac gatgacgtcg aatcgaccga tcgacggacc gatagggggc gcgatgcccc
     8341 ctatcgcgtt ccggggcctg cgcaaggcgg tgatcacgct ggtcgccgcc ggggcattcg
     8401 ccgcgcccgc gtgcgagcgc cgcgaggtcg gggctccctc catcgacccg gggccggtgg
     8461 tggcccgcat caacggccag ccgttgtacc gcaccgcact cgacgcctat cttcccgaag
     8521 acgagttcgc gctttccacc atcgaagagc gacgcaccta tttcgaccgg tgggtggcga
     8581 cccagcttct ctacgaagag gccgcgcgcg tcggcatggg cgtttccgac gaggtggcgc
     8641 gcaagctcga gcagtacaag aaggatatga tcgccgatcg gctcgtgcag gaagtgctcg
     8701 acgagcgcgc cgtcgtgacg cgcggggaag tgatgcggta ctaccgcaac cacaaggacg
     8761 agttcaacct cgaggtgcgc gtgagtcaca tcctgaccaa caccatcgaa gaagcgcagg
     8821 aagcgcaaga gatgctcaag acgcgcccgt tctctctggt ggcccgcaag atgtcggtcg
     8881 acaagcacac cggcgcgggc ggcgacctcg gctatctctc caagggcaac atgctgccgg
     8941 agttcgaaga cgtcgtgttt cgcatgcgcc gcggcgaggt gagcgacatc gtggagtcgg
     9001 aattcggcta ccacatcatc aagctgacgg acgtgcggac ttcgctcaac gagcttccgt
     9061 tcgagcaagt ggcacccgag atctcgcgca cactcttgat ccgaaaacgc gcggcggtgt
     9121 acgacagcct ggtgaccgcg ctggtcgaaa aggcgcgcat cgaggtcatc gaccccgatc
     9181 tcaaattcgc gatcgagcgg gcggaatcgc tgcggacggc gcggctggcg ggagagcagg
     9241 cggcgcgcga gttcaggaag gcgctgcccg agccggggga aggacgcgcg agcagcgcgt
     9301 cgtcatcgaa tggcacccga gcaacggctc cgcccgacac cgcggtcgtg gccccgccgg
     9361 aagcggcgcc ggcgcaggag cccgaaagca ccgagaaggc ggacagcagt ggcggcgagt
     9421 aggcgttcga tttcacgctg cgcgatcgcg gcgttgctgc tcgtcgcagc cgcgatggtc
     9481 cccgcgaacg cgttcgcgca gacccgcatc atcgaccgca tcgtggccgt cgtcgacgac
     9541 gaggccatct tcgagagcga cgtcgagcag gcggtccggc aggcgttggt tcagcgcggc
     9601 caaaccagca ttccaaccgc cgagcgccag cagctcttcg aagaagccct cgcggtcctg
     9661 atcaacgacc ggctcgtcat cgcgcaggcc gctcgtctcg ggatcgacgt ccccttctcc
     9721 gacgtcgaag cggaagtgaa caaggccctc gacgagaatc gcgccgccct gggtggcgat
     9781 gacgcgttca acgcccagct ggccgcggaa ggactcacgc tcgaggacct gaagaagatg
     9841 taccgcgtgc agatcaagaa ccgcatgctg gtcgagcgcg tgttgcgccg cgacatggat
     9901 cgcacgcgcg ccgagccgac cgaggaggag ctgcgcgcgc tcttcgagtc gcgcaagtcc
     9961 gagctcccgg agcgacccga agtcgcgcac ctgcagacca tcttcatcgg cttcgagacc
    10021 tcgtcgtcgg cgtcgagtgc gacgcgcgcc aagatcgaga cgctgcgggc gcgcatcgag
    10081 ggcggcgaag acttcggcga agtggccaag aaggaatccg aagacccgag cgccgcgctg
    10141 ggcggcgact tgggcttcct gcgccccaag gacctgcgcg agcccgcgtt cgccaacgcg
    10201 gtcagttcgc taaaaatggg cgaagtgagc cagccggtac tgaccgtgta cggctaccac
    10261 ttgatccaga tcaccgacga gcgcaaggcc accggcgaag tacgcgtgcg gcacatcctc
    10321 gtgcgcgcgc aggccaccga caccgacatc gacgaagtct tcaagaccgc caacttcatc
    10381 cacgaggaag tgaagcgcgg cgttcctttc gactcgctcg ccgcgcgcta caacaccgat
    10441 ccggcggcgg gaaagaacgg cgacctcggc tgggtgcgcg tgaccgagct cccgcagttt
    10501 ttccaggacg tcttgaagga gatgaagccg ggagacgtga gccaggtgct gcgcgaatcg
    10561 tcgggctttc gcatcgtcaa gctcatcgac cgcgaaaccg cgcgccccta cgagtacgcg
    10621 gaagtccgcg acgaagtgaa gcgcctctac gagcaggaac gcttcgccac caactacgag
    10681 aactacctgg ccgacctacg caagaaattc cacgtcgaga ttcgcagtta gggcagcgac
    10741 tcgcgcagcc ggtgcgagag ccgctcggcc tgcacgcgca gctccggcac cgccgtcgat
    10801 tcccaatcgt ccgcgcgccg cggactcccc agggtataca gcaccggcga cgcgcgtccg
    10861 tgcgcgtcga cgagagcccc gctcgcgtcc gtgacgacac ccagcccgag tgaatccggc
    10921 accagagcgc cgcgctcgcg cattgccacg accagcggct cgcgcaatcg cgcgaagtcg
    10981 ccgtcgggcc ccgtgcaatt gacgaccgcc ccggcgttgt agcgttccgt catagcagag
    11041 ccgcgcgggc gtacggtcac cgaaatcgcc gtcgcgctcg ccgttgcctc cgccacccgc
    11101 cccgccacga tcgaaagctc gccgcgcgcg atcagcccgt tgatctcttc ggcgacgcgc
    11161 gtcgacatac ggtggcgagg cgtctcccaa aacgaccgca cgtggcgcac gaagcgcgc
//
LOCUS       DASPGK010000336         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_903149, whole genome shotgun sequence.
ACCESSION   DASPGK010000336 DASPGK010000000
VERSION     DASPGK010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903149"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VEC56_09420"
     CDS             <1..1227
                     /locus_tag="VEC56_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXV14411.1"
                     /translation="PISMMMFTEEISSADGAFRRSRVESSLSSFSAAAVLEGDSVRYE
                     SKVGGATVRKTIAWDPAACSEALATERVRDWLARAAPETTVVLFDVSEGSFRKARMVR
                     GETTTVEGVRLTAVDEYDDGADTPSSTTWYDSHGDVNRTLVRQLGIEIEIARVSQEEL
                     ATIEIEPDFDIIRQSMLRCDNFPTPVAKVDRVPLRLDFPGTPPVAPMDGPNQKERSRE
                     GRSVTLVLTRDTVNRQTTNGAGLAAFLKSDRFIQSDAAAVRAVADSIRAAAHVDGWPL
                     ARAIAQWVNEYIADKSMEHGYASALDVLRSRSGDCTEHSLLTVALLRAAGIPARPVVG
                     LAFGEAERAFVGHMWVEAYVDGWRTLDALDLRLDPIRLRVHAPSASESLGERDLMRAY
                     GAIAGVTITALAVDAD"
     gene            1242..2630
                     /locus_tag="VEC56_09425"
     CDS             1242..2630
                     /locus_tag="VEC56_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXV14412.1"
                     /translation="MPSLKKTMFREYDIRGRVSDDELNAKSAEWVGRGFGTMLARKGI
                     DDMVVGYDSRFGSVEIKDGLVKGLRASGRNVIMVGMCLTPMMYWSQYRFKVKGGAMVT
                     GSHNPKGWNGLKLAAGFSSTLVASEIQELYDIIAREDFVDGRGDYAEATIFCDYATDL
                     GARVKIKRPLKVVIDTGNGTAGAFAPRIFRDAGLHVVEQFTELDPDFPNHEPDPARTD
                     TVEALGSRVRAEGADLGLAFDGDGDRFGVVDEKGDVIWPDRYMILLARQVLERVPGGK
                     IVFDVKCTQALEDDIHAHGGVPIMWKTGHSHIKKKLQEEKAALAGEMSGHVFFVENYY
                     GFDDGIYGGLRFVEYVAARGEPLSRIMAGTPSYLSTPAINVKCADEVKYGVVERLTAD
                     LKKDFARVVDINGARVVFDDGWGLVRASSNLPELVLRFEAKTPARLNEIKKLFRGYLE
                     KYPEVDRHWENE"
     gene            2642..4447
                     /locus_tag="VEC56_09430"
     CDS             2642..4447
                     /locus_tag="VEC56_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXV14413.1"
                     /translation="MRTPVVIVMCVLAALPAVAQDLPEGYAPLSETQPIVDKTLRVQL
                     APDVSALSAAEKSVVATLIEAGEIFQRLHENMRHHQATESHILLSDLDKRLGSPPATQ
                     NLLALYYASKGPVVRGLDNARRPMLPVDAPVPGGAVYPWGVTKDEIDAFLAAHPEELE
                     SILHPRTVVRRALKHEIDGDLAALNRHRALSHAHPGLKEKLIGLSRHPGQVAFYAVPY
                     AVAYADDLVKVSGLLDRAASAIAAEDADFAGYLRARSQDLIRNDYAAGDSAWVTGHFK
                     NLNAQIGSYENYDDELYGVKTYFGLNVLVRDRARSDALRTATKELQRFEDSLPYNDGK
                     PHKRVRTDIPVGVYDVVADFGQSRGANTATILPNEAGAARKWGRTILLRRNIMADPGL
                     FEMNRVAYAAAVAPEFKDHLTAESNTQRTLWHEIGHYLGVDRTTDGRELDTALENASS
                     VLEEMKADLVSLYLAPELERMGYYTKDARRSLYASGVRRILQKNKPERAQVYQTMQLM
                     QWNYYLARGALSFDAKTGKLVPSYDRFHDAVAAMLRETLAAQTAGDPQAAEAFISKWS
                     EWRPDLHERVAQAMRESERYRFAYVTYAALASAGQ"
BASE COUNT          806 a         1538 c         1542 g          709 t
ORIGIN      
        1 cccatttcca tgatgatgtt cacggaagag atttcgagtg ccgacggcgc gtttcgccgc
       61 tcgcgcgtgg agagctcgct atcgagcttc agcgcggcgg cggtgctcga aggcgattcg
      121 gtgcgctacg aatccaaggt gggcggggca accgtgcgca agaccatcgc gtgggacccg
      181 gccgcgtgtt ccgaggcgct cgcgaccgag cgcgtgcgag actggctcgc gcgtgccgcc
      241 cccgagacga cggtcgtgct cttcgacgtc tcggaaggga gtttccggaa agcgcgcatg
      301 gtgcggggcg aaactacgac ggtcgaaggc gtccgcctga ccgccgtcga cgagtacgac
      361 gacggggccg acacgccgtc gtcgaccacg tggtacgact cgcacggcga cgtcaaccgc
      421 acgctggtgc gccagctcgg catcgagatc gagatcgcgc gcgtctcgca ggaagagctg
      481 gccacgatcg agatcgagcc ggacttcgac atcattcgac agtcgatgct gcgctgcgac
      541 aatttcccga ccccggtggc caaggtcgac cgcgtcccgc tgcggctcga ttttcccggc
      601 acgccgcccg tggcccccat ggacgggccc aaccagaagg agcgctcgcg cgaggggcgc
      661 tcggtgacgc tcgtcctcac gcgcgacacc gtcaatcgcc aaacgaccaa cggcgccggg
      721 ctcgccgcgt tcctcaagtc cgaccgcttc atccagtccg acgccgccgc ggtgcgcgcg
      781 gtcgccgaca gcattcgcgc cgctgcccac gtggacgggt ggccgctcgc gcgcgcgatc
      841 gcgcaatggg tcaacgagta catcgccgac aagagcatgg agcacggcta cgcgtccgcg
      901 ctcgacgtct tgcgctcgcg ttcgggcgat tgcacggagc actcgctgct cacggtcgcg
      961 ctcctgcgcg cggcgggcat tccggcgcgc ccggtcgtgg gcctcgcgtt cggagaagcc
     1021 gagcgcgcgt tcgtcggaca catgtgggtc gaagcgtacg tcgacgggtg gcgcacgctg
     1081 gacgcgctcg atctgcggct cgaccccatt cgcctgcgcg tacacgcgcc ctcggcgagc
     1141 gaatcgctcg gcgagcgcga cctgatgcgc gcctacggag ccatcgccgg cgtcaccatc
     1201 accgcactcg cggtcgacgc cgactagccg ggagggttgc tttgccgtct ttgaagaaga
     1261 ccatgttccg cgagtacgac attcgcggcc gcgtatccga cgacgagctc aacgcgaaga
     1321 gcgcggaatg ggtcgggcgc ggcttcggga ccatgctcgc gcgcaagggc atcgacgaca
     1381 tggtggtcgg atacgacagc cgcttcgggt cggtcgagat caaggacggc ctggtcaagg
     1441 ggctgcgcgc gagcgggcgc aacgtgatca tggtgggcat gtgtctcaca cccatgatgt
     1501 actggtcgca gtaccgcttc aaggtcaagg gcggcgcgat ggtgaccggc agccacaacc
     1561 cgaaagggtg gaacggtctc aagctcgcgg ccggtttttc gtccaccctg gtcgcgtccg
     1621 agatccagga gctatacgat atcatcgcgc gcgaggattt cgtcgacggg cgcggcgatt
     1681 acgcggaagc cacgatcttc tgcgactacg cgacggactt gggcgcgcgc gtgaagatca
     1741 agcgcccgct caaggtcgtc atcgataccg gcaacggcac cgcgggcgcg ttcgcccccc
     1801 gcatcttcag ggacgccggg ctccacgtgg tcgaacagtt caccgaactc gaccccgact
     1861 ttcccaacca cgagcccgac cccgcgcgca ccgacaccgt cgaagcgctc ggaagccgcg
     1921 tgcgcgccga gggcgcggac ctgggtctcg cgttcgacgg cgacggcgac cgcttcggcg
     1981 tggtggacga gaagggcgac gtgatctggc ccgaccgcta catgatcctg ctcgcgcgcc
     2041 aggtactcga gcgcgtcccg ggcgggaaga tcgttttcga cgtgaaatgc acgcaggcgc
     2101 tcgaggacga cattcatgcc cacggcggcg tccccatcat gtggaagacg gggcactcgc
     2161 acatcaagaa gaaactgcaa gaagagaagg cggcgctcgc gggcgagatg agcgggcatg
     2221 tcttcttcgt ggagaactac tacggcttcg acgacggcat ctacggcggg cttcggttcg
     2281 tcgaatacgt cgcggcgcgg ggcgagccgc tctcgcgcat catggccggg acgccgagct
     2341 acctatcgac gcccgcaatc aacgtcaaat gcgccgacga agtgaagtac ggcgtggtgg
     2401 aacggttgac cgccgacttg aagaaggact tcgcgcgcgt ggtcgacatc aacggcgcgc
     2461 gcgtggtgtt cgacgacggc tgggggctgg tgcgcgcatc gtcgaacctg cccgagctgg
     2521 tgctgcgttt cgaggccaag acgcccgcgc gactgaacga gatcaagaag ctgttccgcg
     2581 gctatctcga gaagtacccc gaggtcgacc gccactggga gaacgagtag gaggactcgc
     2641 catgcgtacg ccggtcgtga tcgtgatgtg cgtgttggcc gcgctgccgg ccgtcgcgca
     2701 ggacctcccg gagggatacg cgccgctctc ggagacgcag ccaatcgtcg acaagacgct
     2761 gcgcgtgcag ctcgcccccg acgtgtcggc gttgtcggcg gcggagaaat ccgtggtcgc
     2821 gacgttgatc gaagcgggcg agatcttcca gcgcctgcac gagaacatgc gtcaccacca
     2881 ggcgaccgag tcgcacattc tcctttcgga tctcgacaag cgacttggct ctccgcccgc
     2941 gacgcagaat ctcttggcgc tctactacgc gagcaaagga cccgtggtac ggggactcga
     3001 caacgcacgg cgtccgatgc tgcccgtcga cgcgccggtg cccggcgggg cggtctaccc
     3061 gtggggtgtg acaaaagacg agatcgacgc gttcctcgcc gcccatcccg aagagctgga
     3121 gagcatcctc cacccgcgga cggtggtgcg gcgcgcgctg aagcacgaga tcgacggcga
     3181 tctcgccgcg ctcaatcgcc accgcgcgct ttcccacgca cacccgggcc tcaaggaaaa
     3241 attgatcgga ctctcacgcc acccggggca ggtcgccttc tacgccgtgc cctacgcggt
     3301 cgcgtacgcc gatgatctgg tcaaggtgag cgggctcttg gaccgcgccg ccagcgccat
     3361 cgcggccgag gacgcggact tcgcgggtta cctgcgcgcg cggtcgcagg acttgattcg
     3421 gaacgactat gctgccggcg actcggcctg ggtgacgggc cacttcaaga acctcaacgc
     3481 acagatcggc tcttacgaga actacgacga cgagctctac ggcgttaaga cctacttcgg
     3541 cctgaatgtg ctcgtgcgcg atcgcgcgcg cagcgacgcg ttgcgcacgg cgaccaagga
     3601 gttgcagcgc ttcgaggatt cgctcccgta caacgacggc aagccgcaca agcgcgtgcg
     3661 caccgacatt cccgtcggcg tgtacgacgt agtcgccgat ttcggccagt cgcggggagc
     3721 caataccgcc accatccttc ccaacgaggc gggcgcggcg cgcaagtggg gccgcaccat
     3781 tctcctgcgc cgcaacatca tggcggaccc gggactcttc gagatgaacc gggtcgcgta
     3841 cgcggccgcg gtcgcacccg agttcaagga ccacctcacg gccgaatcga acacgcagcg
     3901 cacgctgtgg cacgagatcg ggcactacct gggcgtcgat cgcaccaccg acggccgaga
     3961 gctggacacc gccctcgaga acgcctcgag cgtgctggag gaaatgaagg ccgacttggt
     4021 ctcgctctac ctggcccccg agctggagcg catgggctac tacaccaagg acgcgcgtcg
     4081 ctctttgtac gcgagcgggg tgcgccgcat tctccagaag aacaagccgg agcgcgcgca
     4141 ggtgtatcag accatgcagc tcatgcagtg gaactactac ctcgcgcgcg gcgcactctc
     4201 gttcgacgcg aagacgggca agctggtccc gagctacgat cgcttccacg acgccgtcgc
     4261 cgcgatgctg cgcgaaaccc tcgcggcgca gacggccggc gacccccagg cggccgaggc
     4321 gttcatctcc aaatggagcg agtggcggcc cgacctccac gagcgcgtgg cgcaggcgat
     4381 gcgcgagagc gagcgttacc gcttcgcgta cgtgacctac gccgcgctcg cgtccgcggg
     4441 ccaatgacgg cgtccaccga cgaacgtgcc acgcggcgcg ctgagctaca gatcgtcgcc
     4501 ggcagcgtgc tgatctcgtt ctcggccgtg ttcgtcaagc tggtcgacgt gggacccact
     4561 gcgtcggcgg tgtatcgaaa cgtgttcggc gcgct
//
LOCUS       DASPGK010000337         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_435123, whole genome shotgun sequence.
ACCESSION   DASPGK010000337 DASPGK010000000
VERSION     DASPGK010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435123"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1034
                     /locus_tag="VEC56_09435"
     CDS             297..1034
                     /locus_tag="VEC56_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV14414.1"
                     /translation="MPGRFAPERREPALRRHVRVMRRTTLAILRAVGRARFDIEPRIP
                     GRGGILVLMNHQSLLDIPVAGEMVPDAYPRFVTHYRYAKGIPLVSHLIRMMNAIPVYP
                     GKMGKAELDRLAEAARTSQHPIVLFPEGHRTQDGEIRPWKRGALDTFLSAREWTVHVV
                     VIDGLWKSARLPDFVRTLTRVRCRVETAGVFEYDGRGRESHDEIVERMRAAMCDKLSQ
                     MRRAPDAVGVEPQVRTPGRPFESGGAG"
     gene            1041..2099
                     /locus_tag="VEC56_09440"
     CDS             1041..2099
                     /locus_tag="VEC56_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14415.1"
                     /translation="MKEKLRDSNSGDVVRPFPRAGAAPADHVPPEVRDVVELLRDACG
                     RDLVAVVFFGSRLLGTSPGEESAADLVVVVENYLRFYESIGSRLPAARHSGIMAALNR
                     VLPPNIIYLNDPGGLRAGAKCFVVTEYDLGLALSADAKDHFFRGRLAQRVHIVYARSE
                     RDRLALERRIDAARRLTVDWVPIYLRGESFGVLDFCRRLMEVSYAGEIRPEARSRVLE
                     VFQAQASFFRLVYGRVLQDAARDGRLVGEGDRYRLAKRPSWRERRRWAAFFRRSKARA
                     TMRWFKYMLTFDDWLDYIVRKIERRSGVRIELTKSERRFPVILLWPKALRVIRAMRTV
                     EANRAAATAAASVDRGDA"
     gene            2096..2830
                     /locus_tag="VEC56_09445"
     CDS             2096..2830
                     /locus_tag="VEC56_09445"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXV14416.1"
                     /translation="MRTELAVLGGVVAFAFASMLAYRVAGARSSSGDLTGRGGSFVLG
                     FWVRNWFYWLIRPVTRASIAIGLTPLFYNLFAVACGLGSMVAYATGHFPTAGLLVFVS
                     GVADVMDGEVARGTHTADARGAFLDSTLDRFTEFAAFAGLAFHASSGWIAVAIVVALG
                     GSLLVSYTRARGESLGVLCKVGLMQRAERMILLGLGSILDPSVCAGAGWSPGSLVAGV
                     VVVIAAGTVWTSVYRTIWIARALRGR"
     gene            2912..>3835
                     /locus_tag="VEC56_09450"
     CDS             2912..>3835
                     /locus_tag="VEC56_09450"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV14417.1"
                     /translation="MRLRESMLGRLLRPGLFLAACVSLGIAPASADEPRLDPNVAPTF
                     QAVELNLDADQEDYTGSVRIDLRVAKTTRDFLFHAEEMTLDSATLEGKGGAVAVEITD
                     AGDRGTRRATAKSDLAPGDYVLSIRFSKPYNTRAVGLYRAIHDGRGYLFTQMESLDCR
                     KAFPCWDEPVYKIPWQMTITVPLQQEAVTNAPVEKETRGASTKTLVYKRLPPTSSYLI
                     AIAVGPLESVAISGLSVPGRIYTVKGAKALAKHGASITPRILARLEEYFGTKYPYEKL
                     DFVAIPEYWPGAMENAGLVTFSDKILLIDPAA"
BASE COUNT          571 a         1248 c         1332 g          684 t
ORIGIN      
        1 ggtcgcggcc gactggtccg gcgcgggaac ggggggaata gaagacgccc cgtcacgtaa
       61 cggtctggat gacatagaga ttttgtctcc tcctcgaagc agtggtacga tactctgccg
      121 cattgatccc ggcgcgtcaa gcgatttagc cggtgcggac ggccaccgcg agccaacccc
      181 gctgcaactt atagttcaac atgatattgc ttagacaact aattggtgcg ttagcgctta
      241 tgctcgtcgg gattgcctac gtcgcgggcg acctttgggg gcgattgggg gtcgagttgc
      301 caggccgctt cgcaccggag cgccgcgagc cggcgctgcg ccggcacgtc cgcgtgatgc
      361 gcaggaccac cctcgcgatc ctgcgagccg tcggccgcgc gcgcttcgac atcgagccgc
      421 gcatcccggg ccgaggcggg atcctggtct tgatgaacca ccaatcgctc ctggacatcc
      481 cggtcgccgg tgagatggtt ccggacgcct acccgcgctt cgtcacccac taccggtacg
      541 cgaagggaat cccgctcgtc tcgcacctca tccgaatgat gaacgcgatc ccggtctacc
      601 cggggaagat gggcaaggcc gagctcgacc ggctcgccga ggccgcgcgc acctcgcaac
      661 acccgatcgt cctgtttccc gaaggacacc ggacccagga tggggagatt cgaccgtgga
      721 agcggggcgc gctcgacacc tttctctccg cgcgcgagtg gacggtgcac gtggtggtga
      781 tcgacggact gtggaagtcg gcgcgtctgc ccgacttcgt tcgcacgctg acccgggtgc
      841 gctgccgcgt ggagacggcg ggtgtcttcg agtacgacgg gcgcggccgc gaatcccacg
      901 acgagatcgt cgaacgaatg cgcgcggcca tgtgtgataa gctctcgcag atgcgtcgcg
      961 caccggacgc ggtcggcgtc gagccgcagg tgcgaactcc agggcgacca ttcgaatccg
     1021 ggggagccgg ctagcgagag atgaaggaga agttgcgaga ctcgaactcg ggcgacgtgg
     1081 tgcgcccatt cccgcgcgcg ggcgcggccc ccgcggacca cgtgccgccc gaggtacgcg
     1141 acgtcgtcga gttgttgcgc gacgcgtgcg ggcgcgactt ggtggcggtc gtgttcttcg
     1201 gctcgagact gctgggcact tcgcccggcg aggagagtgc ggccgacctc gtggtggtgg
     1261 tcgagaacta cctgcgcttc tacgaatcga tcggttcgcg gcttcccgcc gcgcgccaca
     1321 gcggcatcat ggcggcgctc aatcgcgtgc tgcccccgaa catcatctac ctgaacgacc
     1381 ccggcggttt gcgggcgggc gccaagtgct tcgtcgtgac ggagtacgac ctgggcctgg
     1441 cgctgtcggc cgacgccaag gaccacttct ttcgtgggcg cctcgcgcag cgcgtgcaca
     1501 tcgtctacgc gcgcagcgag cgcgaccgat tggccctcga gcgccgcatc gacgcggcgc
     1561 gtcgcctcac ggtggattgg gtgccgatct acttgcgcgg cgaatcgttc ggcgtgctcg
     1621 acttctgccg gcgcctgatg gaggtctcgt acgcgggtga gattcgaccg gaggcgcgct
     1681 cgcgcgtgct cgaggtgttc caggcccagg ccagcttctt tcggctcgtg tacggtcgcg
     1741 tgttgcagga cgccgcgcgc gacggccgcc tggtcggcga gggcgaccgt taccggctcg
     1801 cgaagcgccc ctcgtggcgc gagcgccggc ggtgggcggc cttcttccgg cgctccaagg
     1861 cgcgcgcgac catgcgctgg ttcaagtaca tgctcacctt cgacgactgg ctcgactaca
     1921 tcgtgcgcaa gatcgagcgg cgctcgggag tgcgcatcga gctcaccaag agcgagcgcc
     1981 gctttcccgt gattttgttg tggccgaagg cgctgcgtgt catccgcgcg atgcgcacgg
     2041 tggaagcgaa tcgcgcggcg gccacggctg ccgcgtccgt cgatcgggga gacgcgtgag
     2101 gaccgagctg gccgtcctgg gtggtgtggt cgcgttcgcc ttcgccagca tgctcgcgta
     2161 tcgggtggcg ggcgcgcgct cgtcgagcgg ggatctcacc ggccgcggcg ggtcgttcgt
     2221 gctcggcttc tgggttcgca actggttcta ctggttgatt cggccggtga cgcgggcatc
     2281 gatcgcgatc ggactgacac ctctgttcta taatctgttt gcggtcgcat gcgggctggg
     2341 cagcatggtg gcgtacgcca ccggtcattt tcccaccgcg ggtcttctcg tgttcgtgag
     2401 cggggtcgcg gatgtcatgg acggcgaagt cgcgcgcggg acgcacaccg cggatgcgcg
     2461 cggcgccttc ctcgattcga ccctcgatcg cttcaccgag ttcgcggcct ttgccggact
     2521 ggcgttccat gcctcgtcgg ggtggatcgc ggtcgcgatc gtggtggcgc tgggcgggtc
     2581 gctgctcgtg agctacacgc gcgcgcgagg cgagagcctg ggtgtcctgt gcaaggtagg
     2641 tctcatgcag cgggcggagc gcatgatcct cctgggtctg ggttcgatcc tggacccgag
     2701 cgtgtgcgcg ggcgcgggtt ggtcgccggg atcgctggtc gcgggtgtcg tggtcgtgat
     2761 cgcggccggc acggtttgga cgtccgtata ccggacgatc tggatcgcgc gcgcgctgcg
     2821 cgggcgttga ccgcaactcc caacgggtcg gtcccgtatg atagaatccg ccacgccagc
     2881 agcgtcgctg tcacttcgga ggagatattc catgcggctt cgtgaatcga tgctcggtcg
     2941 cttgctgcga cccgggcttt ttcttgcagc ttgcgtatcg ctggggatcg cgcccgcgag
     3001 cgcggacgag ccccggctcg accccaacgt ggccccgact ttccaggccg tcgagttgaa
     3061 cctcgacgcc gaccaggaag actacacggg ctcggtgcgc atcgacctgc gggtggcgaa
     3121 gaccacgcgc gacttcctgt tccacgccga ggagatgacg ctggatagcg ccactctgga
     3181 aggcaagggc ggtgcggtcg cggtcgagat caccgacgcg ggcgatcgcg gcacgcggcg
     3241 cgcgaccgcg aagagcgacc tcgcgccggg cgactacgtg ctctcgatcc gcttctccaa
     3301 gccgtacaac acgcgcgccg tcggtctgta ccgcgcgatc cacgatgggc gcggctatct
     3361 cttcacgcag atggagtcgc tcgattgccg caaggcgttc ccgtgctggg acgagcccgt
     3421 ctacaagata ccctggcaga tgacgatcac ggtcccgctc cagcaggaag cggtgaccaa
     3481 cgcgccggtc gagaaggaaa cccgcggcgc gtcgaccaag accctggtgt acaagcgcct
     3541 gccgcccacg tcctcgtacc tgatcgcgat cgcggtcggc ccccttgaga gtgtcgcgat
     3601 cagcgggcta tccgtgccgg gccgcatcta caccgtcaag ggtgcgaagg cgctcgcgaa
     3661 gcacggcgca tccatcacgc cgcgcatcct cgcacgactc gaggagtact tcgggaccaa
     3721 gtatccctac gagaagctcg acttcgtcgc catccccgag tactggccgg gtgcgatgga
     3781 gaacgccggc ctggtcacct tcagcgacaa gattctcttg atcgatcccg ccgcc
//
LOCUS       DASPGK010000338         9781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1369844, whole genome shotgun sequence.
ACCESSION   DASPGK010000338 DASPGK010000000
VERSION     DASPGK010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369844"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VEC56_09455"
     CDS             complement(<1..204)
                     /locus_tag="VEC56_09455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14418.1"
                     /translation="MKLAFYFFSLGGSSGGAERMLWEVARAMLERGHDVHVVSWDPPC
                     AQSFYPVPPRARWHRLGDGAGAGV"
     gene            complement(201..923)
                     /locus_tag="VEC56_09460"
     CDS             complement(201..923)
                     /locus_tag="VEC56_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14419.1"
                     /translation="MNAFPLNLRRDVRHSLAECMRRHLRTGDVVYDVGCGTKPFAPAL
                     ASLGVVHVGVDMDNGFYGEDAVDIIGVADALPLRAGVADAVLSSQVIEHLPDPEVAMR
                     EAHRVLKPGGLLFISFPLLFPLHAAPYDFFRYTEHGFQAMCRRGGFEIVEVHEMGGFW
                     YMLSVFGDRYANTLDRSIVKRLRLVPVLSFPARLAWWALHNLEGAAYAAVGKNVRGAR
                     RTWAVNYVYVARRSPAASDPAR"
     gene            complement(920..3139)
                     /locus_tag="VEC56_09465"
     CDS             complement(920..3139)
                     /locus_tag="VEC56_09465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14420.1"
                     /translation="MHGPYALDTGADRRTGMHATDSNREYPLVTIAITCFNAEKTIAA
                     AIAGATAQTWPRLEIVVADDASADRSREIVHELAARDDRIRLITHDTNRGYAGALNSI
                     VAAARGEFIAVFDDDDVSTPERIERQWRRLTDYEARTGTRRVLCYSNRDVIVGDEGTR
                     ADPVRAIGRAAPEPHGTDVVDFLLWHREDPAFVWGQMGSCTLLARTATLREVGPFDES
                     FRRGAEWDFAVRHGLSGGHFIAVDEPLITQRKTATADKSGKIPLTHTVRLRTKHREYL
                     RARGVYLAALAIAHARFHYARGERLRSYWYLGLACLCSPWRVLPNEFRKWKRSRATSL
                     RPLARNAGILFALRALQHVLRLAMLYFVVRALDTAQFGQYQFVLTCVTLATVCALPGL
                     NNSLMQSIARGFLGTFRPAVRLAFRASFLGSAALLVLALWYSRDRASELPRAFVIAAA
                     LFPFAYGLEQWKSLRTGVEDFAGFFRMEGATAVALAVAMIAAVVLWPGAIVVPVAALL
                     GVQSVANSFQTARALRRIPRDASFERGVLGHGTRTTVYSGLNTLANQIDKILIFAFLS
                     PASLAVFVAAERIPEMTKNAVQDMAAVLAPRFAKRARYDHALDRSLRVLGIATGATIV
                     VAAFTLLPWLIGIVFGESYRGAIPYAQAIMCTVAIGNVSTLRYRYVTSKLDESGPLTV
                     NVVMSATRIAASAILVPWLGLVGAVVSTAVYRLVMVAVVDVVIRRRYLRPPGEAVVP"
     gene            complement(3118..3972)
                     /locus_tag="VEC56_09470"
     CDS             complement(3118..3972)
                     /locus_tag="VEC56_09470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXV14421.1"
                     /translation="MPSTSFLQTAREWRARRRLVTYLRAHQHSRAVVEFRGCRFFAGL
                     DNKIEASLLRGERGYDHANFTAATHFVRPGHVCFDIGANIGVYSVVMARLSGSAANVH
                     AFEPVEHIRERLLGNAGLNGYSDLRVNAFALGAEAGSLEMFQVKRGHFRGGTSTFLRN
                     ENVATLGEGQFETRTVQIRTLDHYVAEAGLARVDFMKIDVEGFELPVLEGAARTVARF
                     HPVVLMEYDEARHGRQGHGDKLRTFLNGHGYHAYEFTSFGDDLVLSPFDFSGQPRNRN
                     LLCTARTR"
     gene            complement(3981..4688)
                     /locus_tag="VEC56_09475"
     CDS             complement(3981..4688)
                     /locus_tag="VEC56_09475"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV14422.1"
                     /translation="MTPTPAQNPSLWMKLTRYSFLKRKRSTRNLERLCREFASERRTL
                     VIHSEDVDYRPYFPNAFAVTKRPDKPADLHVDLYYTDLRSIASESYEVILCTGLMEHV
                     PDPQRLVDELHRILKPGGELIVSASAAFSIHEGPNDFFHFTPFSFRLLFARWSRIRML
                     RGSSQPFETVGILLQRILLQCRVFPPARPLVELMCLAVPLLDKCVLSQYSSVGTSAAE
                     FEIDSMLPSNIQAVVVK"
     gene            complement(4685..6226)
                     /locus_tag="VEC56_09480"
     CDS             complement(4685..6226)
                     /locus_tag="VEC56_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV14423.1"
                     /translation="MSIIENVNQFGDDALHAAVCVVGSGPAGAVVAVALASAGVDVLV
                     LEAGSTQPSHDLASTLGRLNVDGEADVRFGASRQLGGATNLWSGRLAPLEPIDLERRD
                     WVPYSGWPLSWGELERYYARAAEVLGIPGHRHFAEGDSPGLATGMEAKVFQWAKHAFS
                     AREYLTNAARANDRLRVVLDAPVRRLIEGANARVVEAAEVGLAGGRTATVRARVFVVA
                     AGGIESARLLLNSTSVRPHGIGNDRDVVGRYFSTHPKANMASLLGSERVSTRHPLFSD
                     WPVADGIVRHGLGFDAATQREHRLLNHYVQLLPLFEHTANRLFESIKGSRTIQSPLVD
                     RAPLIRGVLPGLGLLVFDAMGRLGGVQRRAKTFVLRGFLDQYPNPDNRVTLSDQMDAT
                     GMPKADIAWTYSGADRASVVAFFAAMDRAARAHARARVEYEPLKTSSHWPLTALHSHF
                     MGTTRMGDDPASSVTDAHGRVHGSENLFVAGPSLFPTYGYANPVYTIVALSLRLADHM
                     KEKLV"
     gene            complement(6273..7013)
                     /locus_tag="VEC56_09485"
     CDS             complement(6273..7013)
                     /locus_tag="VEC56_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXV14424.1"
                     /translation="MTLPAFARRPYHRALRALLPLFGLDFGWYEAFHTKWPYALPAVE
                     AWLQHARPRSRSRRFFAAYPLERFARPGDTVLDIGANIGAVSTHLLRLGYVVHAYEPD
                     ARCAEFLRRRFARIDPSRFRLHHQAVSNYTGVATLYRGTRTTESSSILEHKPGTEQAA
                     GYQVTVRSIGDVLDSVEHASLILMDVEGAEYAILNEMLTPRNRDKFGLCIAESHARKI
                     PGLEAEHGRVLEAVERLGMQDRVLLDWH"
     gene            complement(7010..8113)
                     /locus_tag="VEC56_09490"
     CDS             complement(7010..8113)
                     /locus_tag="VEC56_09490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV14425.1"
                     /translation="MKLLFGIKSMNVPGGGAERVLATVVSGLAARGHDVAVLTFDAPG
                     GTSFYPMDARVRRIALGLGSTDRKTGARDFVARLPALRRALRRERPQVAVGFMHSMFV
                     PLSLASVGLRIPMVASEHTVPEYYRNRRWEYLLLVLAGLRSARITVPSDAIRERMPAL
                     VRARTATIPNPVAVPAPTAARTGGTQTVLAVGRLDADKDHATLIDAFATLAERFPDWR
                     VRIVGEGNLRGALAHQISSLGLDARVTLAGATPDIAREYAEAEVLAVPSRYESFGMAT
                     AEALATGVAVVGFADCPGTNELVRHGENGWLVDGADATRVSAFANGLAALMSDSDLRT
                     RLGTAAARDAIRFDPASVVSAWERHLARVGGIA"
     gene            complement(8110..9219)
                     /locus_tag="VEC56_09495"
     CDS             complement(8110..9219)
                     /locus_tag="VEC56_09495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14426.1"
                     /translation="MRVLFVCRMFDRVAGGVERMAIALMNDLAERGHDVSLFTWDRAN
                     AQPYYPMAAAIHWHRLDMGDPSRTANALSRGLRMRRFRSLALADPPEVVVAFQHGPFL
                     FAALSLLRTGIPVVLAERNAPEQLDHTRAGRHRRAIFATMRLARAITVQFEGYVQRYP
                     AYLRARIVPIANPVYGAKSFAVPDADKAQRVLLSVGRLSYQKNHAALVRAFALLSPSF
                     PEWQLRIVGDGEERAALQALSESLGVAARVSWPGAVRDVAVEYRAADLFCMPSRWEGF
                     PNALAEAMAHGLPVVGFRHCAGMAELVIPGHNGELADGNGDPGALAAALEPLMKDAAR
                     RGRMGLEARRIAEVYRPSKSFDDWENLLSQVARAS"
     gene            complement(9221..>9781)
                     /locus_tag="VEC56_09500"
     CDS             complement(9221..>9781)
                     /locus_tag="VEC56_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HXV14427.1"
                     /translation="PNGYRMRIDAIGSDGRAPAGREVADYLLFNRREPGVFYGGGTPA
                     CALMAPLDAFRAAGGFDPGLRRSEDADFAVRLAFAGGHFVGCRERLYLQHATQAPDKS
                     AARNFEAEMRLLDKHRAYLEEQDRYAFACNWFTVRFHHFNHDHGRMALALMRGWLGHP
                     VLVTRQLFTTVPARFFHERRSSRAGR"
BASE COUNT         1683 a         3265 c         3442 g         1391 t
ORIGIN      
        1 cactcccgca ccggcgccgt ccccgaggcg atgccaacgc gcgcgcggag gaaccggata
       61 aaacgactgt gcgcagggcg ggtcccagct caccacgtga acgtcgtgcc cgcgctcgag
      121 catggcccgc gcgacctccc acagcatgcg ctccgcgccg cccgagctgc cgcccaagga
      181 gaagaaatag aacgcgagct tcatcgcgcg gggtccgagg cggccggcga gcggcgcgcc
      241 acgtagacgt aattcaccgc ccaggtgcgc cgggccccgc gcacgttctt gcccacggcg
      301 gcgtacgcgg ccccttccag gttgtgcagc gcccaccatg ccaagcgcgc cgggaacgag
      361 agaacgggga cgaggcgcag gcgtttgacg atcgaccggt ccaacgtatt cgcgtagcgg
      421 tcgccgaaca ccgagagcat gtaccagaag ccgcccattt cgtggacctc gacgatttcg
      481 aagccgccgc ggcggcacat ggcctggaag ccgtgctcgg tgtagcgaaa gaagtcgtac
      541 ggtgccgcgt gcagcggaaa caggagcgga aacgagatga agaggagacc gccgggtttg
      601 agcacgcggt gcgcttcgcg catcgccact tcgggatcgg gcaggtgctc gatgacctgg
      661 ctcgacaaga ccgcgtcggc cacgccggcg cgcagcggga gcgcgtcggc cacgccgatg
      721 atgtcgaccg cgtcctctcc gtagaatccg ttgtccatgt ccacccccac gtgcacgacg
      781 cccaggctcg cgagcgcggg ggcgaagggc ttggtaccgc agccgacgtc gtagacgacg
      841 tcgccggtcc gcaggtggcg gcgcatgcac tccgcgagcg agtgacgcac gtcgcggcga
      901 aggttgagcg gaaacgcgtt catggcacga ccgcctcgcc gggggggcgc aagtagcgcc
      961 ggcggatcac gacgtcgacg accgcgacca tgaccaaacg atagaccgcg gtggacacca
     1021 ccgcgccgac caggcccagc cacggcacca ggatcgcgga ggccgcgatg cgcgtcgcgg
     1081 acatgaccac gttgaccgtc agcggaccac tctcgtccag cttggaggtg acgtaacggt
     1141 agcgcagggt cgacacgttg ccgatggcga cggtacacat gattgcttgc gcgtacggga
     1201 tcgcgccccg atacgattcg ccgaacacga tccctatgag ccagggcagc agggtgaagg
     1261 ccgccaccac gatggtggct ccggtggcga tgcccagcac gcgcagcgat cggtccagcg
     1321 cgtggtcgta gcgcgcgcgc ttggcgaagc gcggcgccag cacagcggcc atgtcctgca
     1381 ccgcgttctt ggtcatctcc gggatgcgct cggccgcgac gaacacggcc agtgacgccg
     1441 gtgagagaaa tgcaaagatg agaatcttgt cgatctggtt ggcgagcgtg ttgagccccg
     1501 agtagacggt ggtgcgtgtg ccgtgaccca gcacacccct ctcgaagctc gcgtcgcgtg
     1561 gaatgcggcg cagcgcgcgc gcggtttgaa aggaattcgc gaccgactgg acgcccaaca
     1621 gcgccgccac gggcacaacg atggcgccgg gccacagcac gaccgcggcg atcatcgcca
     1681 ccgccaatgc gacggcggtc gcgccttcca tgcgaaaaaa gcccgcgaag tcctcgacac
     1741 cggtgcgcag cgacttccac tgctcgagcc cgtacgcaaa cgggaacagg gcggccgcga
     1801 tcacgaaggc ccgcggcagt tcactcgccc ggtcgcggga gtaccacagc gcgagcacga
     1861 gcagggccgc gctgcccaag aagctggcgc ggaacgcgag ccggaccgcg ggacgaaacg
     1921 tgcccaggaa gccgcgcgcg atcgactgca tcagcgagtt attgaggccc gggagcgcgc
     1981 acaccgtcgc cagggtgacg cacgtgagaa cgaactggta ctggccgaat tgcgcggtgt
     2041 ccagcgcgcg cacgacgaag tacagcatcg caaggcgaag cacgtgctgg agcgcgcgca
     2101 gcgcgaacag gatgccggca ttgcgcgcga gcgggcgcag cgaggtcgcg cgggatcgct
     2161 tccacttgcg gaattcgttg ggcagcaccc gccacgggct gcataagcac gccagcccga
     2221 ggtaccagta gctacgaagc cgctcgccgc gggcatagtg aaagcgggcg tgggcgatgg
     2281 ccagcgccgc caggtaaacg ccccgcgcgc gcaggtactc gcggtgcttc gtgcgcagcc
     2341 gaactgtgtg agtcaggggt atcttgcccg acttgtcggc ggtggcggtc ttgcgctgcg
     2401 tgatcagcgg ctcgtcgacg gcgatgaagt gaccgcccga gagtccatgg cgcaccgcga
     2461 agtcccactc cgcaccgcgc cgaaagctct cgtcgaacgg gcccacctcc cggagcgtcg
     2521 ccgtgcgcgc gagcagcgtg cagctgccca tctgtcccca cacgaaggcg gggtcctcgc
     2581 ggtgccacaa gagaaagtcg accacgtcgg taccgtgggg ctccggcgcg gcgcgtccga
     2641 tcgcgcgcac cgggtcggcg cgcgtgcctt cgtcgccaac gatcacgtcg cgattcgaat
     2701 agcagagaac gcgacgcgtg ccggtgcgcg cctcgtaatc agtcagtcgc cgccactggc
     2761 gctcgatgcg ttccggagta ctcacgtcgt cgtcgtcgaa gacggcgatg aactcgcctc
     2821 gagcggccgc cacgatcgag ttcagtgcgc ccgcgtagcc gcgattggtg tcgtgagtga
     2881 tcaagcgaat gcggtcatcg cgcgcagcca gttcgtgcac gatctcgcgc gaacgatccg
     2941 cggatgcgtc gtcggccacg acgatctcga gtcgcggcca ggtctgcgcc gtggcaccgg
     3001 cgatggccgc cgcaatcgtc ttctccgcgt tgaagcaggt gatggcaatc gtaacgagcg
     3061 gatactcgcg attggaatcg gtggcgtgca tgccggtgcg gcgatcggcg ccggtgtcta
     3121 acgcgtacgg gccgtgcaca gcaggttgcg attgcgcggt tggccggaga agtcgaaggg
     3181 cgagaggacc aagtcgtcgc cgaaggaggt gaactcgtag gcgtggtagc cgtgtccgtt
     3241 caagaacgtg cgtagcttgt cgccgtgtcc ctgccggccg tggcgcgctt cgtcgtattc
     3301 catgagcacg acggggtgga agcgcgcaac cgtgcgcgcc gccccctcca gcaccggaag
     3361 ctcgaagcct tcgacgtcga tcttcatgaa gtcgacacgc gccagtccgg cctcggccac
     3421 gtagtgatcc agggtgcgaa tctggaccgt gcgcgtctcg aactggccct cgcccagggt
     3481 cgcgacgttc tcgttgcgaa ggaaagtact ggtcccgccg cgaaaatggc cgcgcttgac
     3541 ctggaacatc tcgagcgagc ccgcttcggc ccccaacgcg aacgcgttca cgcgtaggtc
     3601 cgagtacccg ttgaggcccg cgttgcccaa aagcctctca cgaatatgct cgaccggctc
     3661 gaacgcgtgc acattggcgg ccgagccgga gaggcgcgcc attaccacgc tgtacacccc
     3721 gatgttggcg ccgatgtcaa agcacacgtg accgggtcgt acgaagtgag tcgcggccgt
     3781 gaagttggcg tggtcgtacc cgcgctcgcc gcgcaacagc gaggcttcga tcttgttgtc
     3841 cagccccgcg aagaaccggc acccacggaa ctcgaccacg gcacgcgagt gctgatgcgc
     3901 gcgcaagtag gtgacgagtc gtctccgggc gcgccactcg cgtgcggtct gtaagaagct
     3961 cgtagacggc atgagaatct ctatttcacc acgacggcct gaatgttgga tgggagcatc
     4021 gagtcgatct cgaactccgc ggccgacgtg ccgacgctag aatactggga aagcacgcac
     4081 ttgtcgagga gcgggaccgc gaggcacatg agctccacga gcgggcgcgc gggcgggaag
     4141 acgcggcact gcaggaggat cctctgcagg aggatcccca ccgtctcgaa cggctggctc
     4201 gagccgcgca gcatgcgaat acgcgaccag cgcgcgaaca ggagtcgaaa cgagaagggc
     4261 gtgaagtgga agaagtcgtt gggtccttcg tggatggaga aggctgcgct ggcggagacg
     4321 atcaactcgc cgccgggctt caagatgcgg tggagctcgt ccaccagacg ctgcgggtcg
     4381 ggcacgtgct ccatgagccc ggtgcacaga atgacctcgt acgactcgga cgcgatgctg
     4441 cgcaggtcgg tgtagtacag gtcgacgtgc aaatcggcgg gcttgtcggg gcgcttggtc
     4501 accgcgaacg cgttggggaa gtagggccgg tagtcgacgt cctcggagtg gatcaccagc
     4561 gtgcgccgtt cgctcgcgaa ctcgcggcac aatcgctcga ggttgcgcgt ggatcgctta
     4621 cgcttgagga agctgtagcg ggtgagcttc atccagagcg acggattctg cgcgggggtg
     4681 ggtgtcatac cagcttctcc ttcatatgat cggccagtcg cagcgacagc gcgacgatgg
     4741 tgtacaccgg attggcgtag ccgtaagtcg ggaacagcga cggcccggcc acgaacaggt
     4801 tctcgctgcc gtggacgcgt ccgtgtgcgt cggtcaccga cgacgcgggg tcgtcgccca
     4861 tgcgcgtggt acccatgaag tgggaatgga gcgcggtcag cggccagtgc gaagaggtct
     4921 tgagcggctc gtattccacg cgcgcgcgtg cgtgcgcgcg tgcggcgcga tccatggcgg
     4981 cgaagaacgc gaccaccgac gcgcgatcgg cgccggagta cgtccacgcg atgtcggcct
     5041 tgggcattcc ggtggcgtcc atctgatccg acagagtcac gcggttgtcc ggattcggat
     5101 actggtcgag aaagccgcgc agcacgaacg tcttcgcacg ccgctgcacg ccgcccaggc
     5161 gtcccatcgc gtcgaagacg agcaggccca gcccgggcag cacgccccga atcaacggcg
     5221 cgcgatcgac cagcggcgac tgtatcgtgc gcgagccctt gatcgattcg aagagccggt
     5281 tggcggtgtg ctcgaaaagc ggcaagagct gcacgtagtg attgagcagg cggtgctcgc
     5341 gctgcgtggc cgcgtcgaag cccaacccgt ggcgcacgat cccgtccgcg accggccagt
     5401 ccgagaacag cgggtggcgg gtcgagacgc gctcgctccc cagcagcgag gccatgttcg
     5461 ccttcgggtg cgtcgaaaag tagcggccca ccacgtcgcg gtcgttgccg atgccgtgcg
     5521 gccggaccga ggtcgagttc agtagcaatc gcgcggactc gatgccgccc gcggccacga
     5581 cgaacacccg cgcgcgcacg gtcgcggtgc ggccgcccgc gagcccgacc tcggcggctt
     5641 ccaccacgcg cgcgttggcg ccttcgatca accggcgcac gggtgcgtcg agcaccacgc
     5701 gcagccggtc gttcgcccgc gcggcgttgg tcaaatactc gcgcgcgctg aacgcgtgct
     5761 tcgcccactg gaacactttg gcttccatgc cggtcgcgag gccggggctg tcgccttcgg
     5821 cgaagtggcg gtggcccgga atgccgagga cctcggccgc gcgcgcgtag tacctctcga
     5881 gctcgcccca cgacagcggc cacccgctgt acggaaccca gtcgcgtcgc tcgaggtcga
     5941 tgggctcgag cggggcgagt cgcccggacc agaggttggt ggcaccgccg agctggcgcg
     6001 aggcgccgaa gcgcacgtcc gcctcgccgt ctacgttcag acgcccgagc gtggaggcaa
     6061 ggtcgtgtga gggttgcgtc gagcccgctt cgagcacgag cacgtcgacg ccggccgacg
     6121 ccagtgcgac ggccaccacc gcgcccgcgg ggccggatcc gaccacgcac accgccgcgt
     6181 gcagcgcgtc gtcgccgaac tgattgacgt tctcgatgat ggacatgtgg aaaattccgc
     6241 gagccgcagc atcgccccgg gcggcgttgc tgtcaatgcc agtccaagag cacgcggtcc
     6301 tgcatgccga gacgctcgac cgcctcgagc acgcgcccgt gctccgcctc gagtcccggg
     6361 atcttgcgcg cgtgactctc cgcgatgcac agcccgaact tgtcgcggtt gcgcggtgtt
     6421 agcatctcgt tgaggatggc gtactcggcc ccttcgacat ccatcaggat cagagacgcg
     6481 tgttcgaccg aatcgagcac atcgccgatc gagcgtacgg tcacctggta tccggccgcc
     6541 tgctcggtgc cgggcttgtg ctcgaggatc gagctggatt cggtggtgcg cgtcccgcgg
     6601 tacagcgtgg ccacgccggt gtagttcgac accgcctggt ggtgcaggcg gaagcgcgac
     6661 gggtcgatgc gcgcgaaacg ccggcgcagg aactccgcgc agcgcgcgtc cggttcgtag
     6721 gcgtggacga cgtagccgag ccggagcaag tgggtcgaca cggcgccgat gttggcgcca
     6781 atgtcgagca cggtgtcgcc cggtcgagcg aagcgctcga gcgggtaggc cgcgaagaac
     6841 cggcgcgagc ggctgcgggg ccgcgcgtgc tgcagccatg cctcgacggc gggcagcgcg
     6901 tacggccact tagtgtggaa cgcttcgtac cagccgaagt ccagcccgaa gagcgggagc
     6961 agcgcgcgca gcgcgcggtg gtagggacgc ctcgcgaacg cagggagcgt catgcgatcc
     7021 cccccacgcg agcgaggtgc cgctcccacg cggagaccac ggacgcgggg tcgaagcgta
     7081 tcgcgtctcg cgcggccgcg gttcccaagc gagtgcgcaa gtcggaatcg gacatgagtg
     7141 cggccaatcc gttcgcgaat gcgcttacgc gggtggcgtc cgcaccgtcc accagccagc
     7201 cgttctcccc gtggcgcacg agctcgttgg tgcccggaca gtccgcgaaa cccaccaccg
     7261 cgactcccgt ggccagcgcc tccgccgtcg ccatgccgaa gctctcgtag cgcgacggga
     7321 ccgccagcac ctcggcctcc gcgtattcac gcgcgatgtc gggtgtcgca cccgcgaggg
     7381 tcacgcgcgc gtcgagcccc agcgaagaga tctggtgggc cagtgcgcct cgcaagttac
     7441 cctcgccgac gatgcgcacg cgccagtccg ggaagcgctc ggcgagtgtg gcgaaggcgt
     7501 cgatcaaggt ggcgtggtcc ttgtcggcgt ccaggcgccc gacggcgagc acggtctggg
     7561 tgccccccgt gcgcgcggcc gtcggcgcgg gaaccgcgac cgggttcggt atcgtcgccg
     7621 tacgcgcgcg cacgagcgcc ggcatccgct ctcgaatcgc atccgagggg acggtgatgc
     7681 gcgccgagcg cagccccgcg agcaccagca ggagatactc ccagcgccgg ttgcggtagt
     7741 actcggggac cgtgtgctcg ctcgcgacca tcggaatgcg caaccccacg gacgcgagcg
     7801 acagcggcac gaacatgctg tgcatgaatc cgacggcgac ctggggccgc tcgcggcgga
     7861 gcgcacggcg cagcgcgggc aagcgcgcga cgaagtcgcg cgcgccggtc ttgcgatcgg
     7921 tcgatccgag ccccagcgcg atgcgccgca cgcgcgcgtc catgggatag aagctcgttc
     7981 cgcccggtgc gtcgaaggtg agcacggcga catcgtggcc gcgcgcggcc aatccggaga
     8041 cgacggtggc gagcacgcgc tccgcgccgc cgccggggac attcatcgac ttgattccga
     8101 agagcagctt cacgacgcgc gcgcgacctg gctcaataga ttctcccaat cgtcgaacga
     8161 cttcgagggc cggtagacct ccgcgattcg gcgcgcttcc aatcccatgc gcccgcgacg
     8221 cgcggcgtct ttcatcaagg gctcgagtgc ggcggccagc gcgccggggt cgccgttgcc
     8281 gtcggccagc tcgccgttgt gaccgggtat caccaactcg gccatgcccg cgcagtggcg
     8341 gaagccgacc acgggcaggc cgtgcgccat cgcttcggcc agcgcgttgg gaaacccctc
     8401 ccaccgcgac ggcatgcaga agaggtcggc cgcgcggtac tcgacggcta cgtcgcgcac
     8461 cgcccccggc cacgacacgc gcgcagcgac accgagcgat tcggagagcg cctgcaatgc
     8521 cgcgcgctcc tctccgtcgc cgacgatgcg cagttgccac tcggggaagg acggtgaaag
     8581 cagcgcgaac gcgcgcacca gtgccgcgtg gtttttctgg tacgagaggc ggccgaccga
     8641 gagcaggacg cgctgggcct tgtccgcgtc cggcaccgcg aacgacttcg cgccgtacac
     8701 cgggttggcg atgggaacga tgcgcgcgcg caagtacgcg gggtagcgct gcacgtagcc
     8761 ctcgaactgg acggtaatgg cgcgcgcgag ccgcatggtg gcgaagatcg cgcgccggtg
     8821 ccgacccgcg cgcgtgtgat cgagctgctc gggggcgttg cgctcggcga gcaccacggg
     8881 gatcccggtg cgcaagaggc tcaaggccgc gaacaggaag ggcccgtgct ggaacgccac
     8941 cacgacctcg ggcgggtcgg cgagcgcgag cgaacgaaag cggcgcatgc gcaatccgcg
     9001 cgacagcgcg ttcgcggtgc gcgacgggtc tcccatgtcc agtcggtgcc agtggatcgc
     9061 ggccgccatc gggtagtacg gctgcgcgtt cgcgcggtcc cacgtgaaca gcgacacgtc
     9121 gtgtccgcgc tcggccaggt cgttcatcag agcaatcgcc atacgctcca cgccgccggc
     9181 cacgcggtcg aacatccggc agacgaacag gaccctcatt ctagcggccg gcccgcgacg
     9241 atcgccgctc gtggaagaag cgcgccggaa ccgtggtgaa gagctggcgc gtcaccagca
     9301 ccgggtgacc gagccaaccg cgcatcaagg ccagtgccat gcggccgtgg tcgtggttga
     9361 agtggtggaa gcgcaccgtg aaccaattgc acgcgaaggc gtagcggtcc tgctcctcca
     9421 aataggcgcg gtgcttgtcg agcaggcgca tctcggcctc gaagttgcgc gcggcactct
     9481 tgtcaggcgc ctgggtggcg tgctgcaggt agaggcgctc cctgcaaccg acgaaatgcc
     9541 cgcccgcgaa cgcgagccgc accgcgaagt cggcgtcctc ggagcgacgc agcccggggt
     9601 cgaagccgcc ggcggcgcga aaggcgtcga gcggggccat caacgcgcac gcgggcgtgc
     9661 cgccgccgta gaacacgccg ggctcgcggc gattgaacaa cagatagtcg gccacctcgc
     9721 gacccgcggg ggcgcgcccg tcggagccga tggcgtcgat gcgcatgcga tacccgttgg
     9781 g
//
LOCUS       DASPGK010000339         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1019345, whole genome shotgun sequence.
ACCESSION   DASPGK010000339 DASPGK010000000
VERSION     DASPGK010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019345"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VEC56_09505"
     CDS             <1..271
                     /locus_tag="VEC56_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HXV14428.1"
                     /translation="SQHRNRDNAMKILRARLYALKLEEEKARKDKMMEGQKDIAWGSQ
                     IRSYVVHPYTMVKDHRTDWQTSDVHGVLDGDLDALIEAYLHKFTG"
     gene            277..1779
                     /gene="lysS"
                     /locus_tag="VEC56_09510"
     CDS             277..1779
                     /gene="lysS"
                     /locus_tag="VEC56_09510"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXV14429.1"
                     /translation="MSEEQERHEESLHGFEKLRATRIEKLEALREDGVNPYPYRFAVT
                     HTVAAVRSGEKSLAEAAAVVRVAGRVMAMRGHGKTAFGHVEDATGRLQFYVRKDDLGE
                     DVFRRFERIEVGDILGVAGTVFRTRTDELTIKVESFEVLAKAIRPLPEKWHGLTDKEI
                     RYRQRYVDLIVNPEVREVFVKRSRLVETMRRFLLSEGFLEVETPVLQPLYGGATARPF
                     TTHHNALDMTLYLRIANELYLKRLLVGGFERVFEFAKDFRNEGIDRSHNPEFTMMECY
                     AAYLDYMDFMDMTERMIARIARELTPDGTVTFGGRVMDFSRPWQRLRYFDALREKTGM
                     EFFNVGEKDLAANAKKLGVFVEKGAPASVIIDEVFGALVEPDLQEPTFIYDYPKALSP
                     LAKDHRSVAGLVERFEGYVGGFEVCNAFSELNDPLEQRRRFEHQMENKARGDEEAHLL
                     DEDYLRALEYGMPPAAGLGVGIDRLTMVFTDSHSIRDVILFPHMRPEDGR"
     gene            1776..>3022
                     /locus_tag="VEC56_09515"
     CDS             1776..>3022
                     /locus_tag="VEC56_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14430.1"
                     /translation="MNWALFIARRYLQTKKRSALVSRITSIAVGGVFVGTMTLVIVLS
                     VINGFETELRQRIVGFNTHVLVFARTPDAWASVDTALTRIEAESEVVATAPFVRSEAL
                     AAYEVIPGRRTKLRGVVVKGVDLAREHAVSSVVDSIRPTIDSFDTSGFEDEVPRLGAV
                     LGLDLALDLGIGLGEEISLVTAPGTVRGSAVEPETHVCRVLGFFNSGMYEFDSRFVYI
                     DIQDAERMFDFETGLRGVSVMVKDMYRAEAFDRRLQEILSTRDYGTNNWIQLNQNLFS
                     YMKIEKILMFLMLTLIILVAAFNLVGMLTMVIMEKRREIGILRSMGASGRGVMSVFMI
                     EGTAIGVLGTLGGLVTGLVACFVLDRIRIELPGDVYFIKTLPVLVQWPDLVLIGLSSV
                     VICFLATVYPSWEASRLAPVEAIR"
BASE COUNT          545 a          928 c         1009 g          540 t
ORIGIN      
        1 ctcgcagcac cgcaaccgcg acaacgcgat gaagatcctg cgcgcgcgcc tgtatgcgct
       61 caagctcgaa gaagagaagg cgcgcaagga caagatgatg gaaggccaga aggacatcgc
      121 gtggggcagc cagattcgct cctacgtggt gcacccctat accatggtca aggaccaccg
      181 caccgactgg cagacgagcg atgtccacgg cgtcctggac ggcgacctgg acgcgctcat
      241 cgaggcgtac ctgcacaagt tcacgggcta accaccatgt cagaagaaca agaacggcac
      301 gaagagagct tgcacggctt cgagaagttg cgcgccacgc gcatcgaaaa gctggaggcg
      361 ctgcgcgagg acggcgtcaa cccgtatccg taccgcttcg cggtgacgca cacggtggcc
      421 gcagtgcgct cgggcgagaa gtcgctggcc gaggcggccg cggtcgtgcg cgtcgcgggg
      481 cgcgtgatgg cgatgcgcgg gcacggcaag accgccttcg gacacgtcga ggacgcaacc
      541 gggcgcctgc agttctatgt gcgcaaggat gacctcggcg aggacgtgtt tcgccgcttc
      601 gagcggatcg aggtgggcga tatcctgggc gtcgccggca cggtgttccg aacccgtacc
      661 gacgagctca cgatcaaggt cgagtcgttc gaggtgttgg ccaaggccat ccggccgctg
      721 ccggagaaat ggcacggact caccgacaag gagatccgct accgccagcg ctacgtcgac
      781 ttgatcgtca atccggaggt gcgcgaggta ttcgtcaagc gctcgcgcct ggtcgagacg
      841 atgcggcggt ttctcctgag cgagggattc ctcgaggtcg aaaccccggt gctgcagccg
      901 ctctacggcg gagccaccgc gcgaccgttc accacccacc acaacgcgct cgatatgacg
      961 ctctacctgc gcatcgccaa cgagctgtac ctgaagcggc tcctggtggg cgggttcgag
     1021 cgcgtgttcg agtttgcgaa ggactttcgc aacgagggta tcgaccgctc ccacaatccc
     1081 gagttcacga tgatggagtg ctacgccgcc tatctcgact acatggactt catggacatg
     1141 acggaacgaa tgatcgcacg catcgcccgc gagctcacac ccgacgggac ggtgaccttc
     1201 ggcgggcgcg tcatggactt ctcgcggccg tggcagcgtt tgcggtactt cgacgcgttg
     1261 cgcgagaaga cggggatgga gttcttcaac gtgggcgaga aggacctggc ggcgaacgcg
     1321 aagaagctgg gcgtgttcgt ggagaaggga gcccccgcct cggtcatcat cgacgaggtc
     1381 ttcggcgccc tggtcgagcc cgacttgcag gagcccacgt tcatctacga ctacccgaaa
     1441 gcgctctcgc cgctggccaa agaccaccgt tcggtggcgg gactggtcga gcgtttcgag
     1501 ggctacgtgg gaggtttcga agtttgcaat gcgttctccg agctcaacga cccgctcgag
     1561 cagcggcggc gcttcgagca tcaaatggag aacaaggccc gcggcgacga ggaggcccac
     1621 ctcctcgacg aagactacct gcgtgcgctc gagtacggca tgccgcccgc ggccggcctg
     1681 ggtgtgggca tcgatcgttt gaccatggtg ttcaccgaca gccactccat ccgcgacgtg
     1741 atcctgttcc cgcacatgcg tcccgaggac gggcgatgaa ctgggcgctg ttcatcgcgc
     1801 ggcgatacct ccaaaccaag aaacgctcgg cgctggtctc gcgtatcacc tcgattgcgg
     1861 tggggggggt gttcgtcggt accatgacgc tggtgatcgt tctctccgtc atcaacggct
     1921 tcgagaccga gctgcgccag cgcatcgtcg ggttcaacac ccacgtgctg gtgttcgcgc
     1981 gcacacccga cgcctgggcg agtgtggaca ccgcgctcac gcgcatcgaa gccgagagcg
     2041 aagtcgtcgc gacggcaccg ttcgtgcgct ccgaggcgct ggcggcctac gaggtgattc
     2101 ccgggcggcg caccaagctg cgcggcgtgg tggtcaaggg tgtggacctg gcgcgcgagc
     2161 acgccgtctc ttcggtggtc gacagcattc gccccaccat cgattccttc gacaccagcg
     2221 gcttcgaaga cgaggtcccg cgcctgggcg cggtgctggg tctggatctg gccctcgacc
     2281 tcggcatcgg tctgggggaa gagatcagcc tggtcacggc ccccgggacc gtgcgcggct
     2341 ccgccgtcga gcccgagacg cacgtgtgcc gcgtcctcgg gttcttcaac agcggcatgt
     2401 acgagttcga ctcgcgcttc gtctacatcg atatccagga cgccgagcgc atgttcgact
     2461 tcgagaccgg gttgcgcggg gtcagcgtga tggtgaagga catgtaccgc gcagaagcct
     2521 tcgatcgacg attgcaggaa atcctttcga cgcgtgacta cggcaccaac aactggatac
     2581 agctgaacca gaatctgttc agctatatga aaatcgaaaa gatcctcatg tttctcatgc
     2641 tgaccctcat catcctggtg gcggccttca acctggtcgg catgctgacg atggtgatca
     2701 tggagaagcg gcgcgagatc ggaatcctgc gctcgatggg cgcgtcgggg cggggtgtga
     2761 tgtcggtgtt catgatcgaa gggaccgcga tcggggtctt ggggacgctg ggggggctgg
     2821 tgaccgggct ggttgcctgc ttcgtgctgg atcgcattcg gatcgagctc ccgggggacg
     2881 tctatttcat caagaccctg ccggtcctgg tccagtggcc cgacttggtc ttgatcgggc
     2941 tctcgtcggt ggtcatctgt ttcctggcga cggtttaccc cagctgggag gcgagccggc
     3001 tcgccccggt cgaggcgatt cg
//
LOCUS       DASPGK010000340         5502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1138077, whole genome shotgun sequence.
ACCESSION   DASPGK010000340 DASPGK010000000
VERSION     DASPGK010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138077"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VEC56_09520"
     CDS             <1..573
                     /locus_tag="VEC56_09520"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV14431.1"
                     /translation="KHLRRHRVVFMFLLGYWFYIDGVHTIVRMGMDYGLSLGFSAGTL
                     IVALLMIQFVGFPATIAFGRMAERVGARTGILVAIVIYIAICVWGYRIQSAWEFFVLA
                     GGIGLAQGGINSLSRSFYSRLIPADESAQFFGYYNTIGRFSAVLGPWVMGSVSLATGN
                     PRLSMLALILFFLAGAAFLLRVRPSAREAS"
     gene            570..1145
                     /gene="pdxH"
                     /locus_tag="VEC56_09525"
     CDS             570..1145
                     /gene="pdxH"
                     /locus_tag="VEC56_09525"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HXV14432.1"
                     /translation="MTLRDLVERFNAEMDRAMTAGVYEPTAASLATMAGRPSVRMVLL
                     KEADERGFVVYTNLESRKGRELVVNPWAALCFWWAPSATQIRVEGRVEVVSDADADEY
                     FAQRPRAKQISAWASHQSSQLGSRQELLDRVARLEDEYRDRSVPRPPYWTGIRVIPDR
                     IEFWYGHEDRLHERFEYRLERGVWIERILSP"
     gene            1297..1740
                     /locus_tag="VEC56_09530"
     CDS             1297..1740
                     /locus_tag="VEC56_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14433.1"
                     /translation="MENRIRPALTVALCMCAALGLTGKSHAFTLHRIAASLDMSHIAS
                     SSALSGATISFDAHHAVVPAVTELTAPSTEIEVRIPTECDVEIQVIDEAGVRVSGARV
                     HMPAGVQKLGFCGRDAQGRALPNGLYYYNVIVGDDVSTTRVVIVR"
     gene            1810..3042
                     /locus_tag="VEC56_09535"
     CDS             1810..3042
                     /locus_tag="VEC56_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV14434.1"
                     /translation="MRDRSDIVVVGAGAAGLMAAISARRANPSRRVVVLDGAKKLGAK
                     ILIAGGGRCNVTHHRVDETAFAGSTRPAIRKVLGRFDVARTVAFFEEIGVTLKREETG
                     KLFPTTDDAHTVLDALLREARRVGVEILHPCRVTAIERASDGFVVTAGAHRLEAERVV
                     IATGGKSIPKSGSDGLGYELVKALGHSITPLVTPGLVPLTLPRDHFLCALAGVTLDVT
                     IELRSGTGKRLESFTNSTLLTHFGLSGPSVLDISRYYLQAIASDSKAGLFANWLPGRD
                     EDALDRELQSLRGKSVGGYLRERLPERLARALCEHAGVDPATRGDALKRDGRRAVAAA
                     ACNLPLPVTGNRGYTYAEVTAGGVPLAQLHLDTMESRVCPGLYLCGEICDVDGRIGGF
                     NFQWAWSSGFVAGTAASK"
     gene            3099..5417
                     /locus_tag="VEC56_09540"
     CDS             3099..5417
                     /locus_tag="VEC56_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14435.1"
                     /translation="MFRRTSPSNWLFLWASALVFAGSLSCSDDPSAPEEPEDRNVSVE
                     SSNRETATIGSAGGSVSTTNNAGITYTLDIPPYALPGDVEIAITPVTAIDNLPFDGRL
                     EGAVRLGPSGLQLIKPAILTIAQAPSASGTERLIGFAYQGDADSFEPAAAIDGTTEIR
                     VPITHFSGAGAASGEQEELEPWIRQMCASIPTRDHTFYEQVELCFDGSVTRRDWLLDL
                     ARDYLDQIVIPLIHTDLSDGVAQYIGWYSGCNLNAQTFNILDWETTLATEIENVQELL
                     ATELVATIAGEKSALCSQGGIEHLYWILHYRYLAQAAGAGDVAGLRDEDILDGLCAEV
                     RIPAVTLADPMPLNQGTSLDARAELWISNQRIDLPVFRFTVNPTTANATCGTGQTICG
                     GNSNELGEYTAVVTRTGPGRVGIDLFAALLIATPSGTVHALAETPLVGSETIERESYS
                     IAATFPTTVEPGTPVTLTAVVTRNNDVGSPQPVEGAVVTFDVDGGVADPVGGQTDASG
                     SVSTQVTANDGPNRVVVDIAAAFDGRQVATKTVEAAIETTEPTGRLVLLSRDTQILAW
                     VPNPDECMGDPGADTRSSELGRVEVTEDANMSCSNATGTGHGQAVNQHVSDPGFAADG
                     SSALLYFFTAGSASVQATGDYVYAASVLGEMLVSFQVLEGTVAYEINAAFNGTIGDVS
                     VTLQGGSTSVNWTKKPGDGNPNPFATSGTLSPGGYVARFRIIAELDSQVAPASGTSGV
                     TATIRLTAAPAAAAGNPTPLSRSPRDRVVRSR"
BASE COUNT          943 a         1852 c         1740 g          967 t
ORIGIN      
        1 aagcacctgc gccggcatcg cgtcgtgttc atgttcctcc tcggctactg gttctacatc
       61 gacggcgtgc acaccatcgt gcgcatggga atggactacg gcttgtcgct cggcttctcg
      121 gccggcacgt tgatcgtggc cctcttgatg atccagttcg tcggcttccc cgcgaccatc
      181 gcattcggta gaatggccga acgcgtcggg gcgcgaacgg gcatcctggt cgcgatcgtg
      241 atctacatcg cgatctgcgt ctgggggtat cgcatccaga gcgcgtggga gttcttcgtg
      301 ctcgcgggcg gcatcggtct cgcgcaaggg ggcatcaaca gcttgagccg gtccttctat
      361 tccaggctga ttcccgccga cgagtcggcg cagttcttcg gctactacaa caccattggg
      421 cgcttctcgg ccgtgctcgg tccctgggtg atggggtcgg tgagcctcgc caccggcaac
      481 ccccggctct cgatgctggc cctgatcctg ttcttcctgg cgggcgccgc cttcctcctg
      541 cgggtgcggc cctcggctcg ggaggcgtca tgacgctgcg cgacctggtg gagcgattca
      601 acgccgagat ggaccgggcc atgacggccg gggtctacga acccaccgcc gcctccctcg
      661 ccaccatggc cggccgcccg tcggtccgga tggtcctcct caaggaggcc gacgagcggg
      721 gattcgtggt ctataccaac ctcgagagcc ggaagggccg cgagctggtc gttaacccgt
      781 gggcggccct gtgcttttgg tgggcgccgt cggcgaccca gatccgcgtc gaaggccggg
      841 tcgaagtggt aagtgacgcg gacgctgacg agtacttcgc ccaaaggccc cgggcgaaac
      901 agatcagcgc ctgggcgtcg caccaaagct cccaactcgg gtcgcgccag gagctcctcg
      961 accgggtggc ccggttggag gacgagtacc gcgaccggag cgtcccgcgc cccccctact
     1021 ggaccggcat ccgggtcatc ccggaccgca tcgaattctg gtacggacac gaggaccggc
     1081 tgcacgaacg gttcgagtac cggctcgagc ggggggtctg gatcgagcga attctgtcgc
     1141 cctgaccact ccggggcaaa gtttcctttg cgcgtccgca tacccctctg ctaaaataca
     1201 ggcatcccac cagttccaga ggccagcccc agtcggccgc tggagacgtc ggagccccga
     1261 agcaagactc ttgtttcgag acggaggaac cgcatcttgg aaaaccgcat tcgccccgcg
     1321 ctcaccgttg ctttgtgcat gtgcgcggcg ctcggcctga ctgggaagtc gcacgcgttt
     1381 accctgcatc gcatcgccgc gtcgctcgac atgagccaca tcgcttcgtc gagtgcgctc
     1441 tccggcgcca cgatctcctt cgacgcgcac cacgccgtcg tccccgcagt cacggaattg
     1501 accgccccta gcaccgagat cgaagtccgc attccgactg aatgcgacgt cgagatccag
     1561 gtgatcgacg aagccggcgt gcgcgtgtcc ggcgcccgcg tccacatgcc cgcgggggtg
     1621 cagaagcttg gcttctgcgg ccgcgacgcg caagggcgcg cgctccccaa cggcctgtac
     1681 tactacaacg tgatcgtcgg cgacgacgtc agcacgacgc gcgtcgtcat cgtgcgctga
     1741 cgagcgtctt tcaaatcgct tccaaataac ggcggcacct gtgccgccgt ttttgtacta
     1801 tcccccgaca tgcgcgatcg cagcgacatc gtcgtggtcg gcgcgggcgc cgcgggtctg
     1861 atggcggcca tctccgcgcg gcgcgcgaat ccctcccgcc gtgtcgtcgt cctcgacgga
     1921 gcgaagaagc tcggcgccaa gatcctcatc gccggcggcg ggcgctgcaa cgtcacccac
     1981 catcgtgtcg acgagaccgc gttcgcgggc tcgacgcgcc cggccattcg aaaagtgctg
     2041 gggcgattcg acgtcgcgcg cacggtcgca ttcttcgaag agattggcgt caccttgaag
     2101 cgcgaagaaa ccggcaagct tttccccacc accgacgacg cgcacaccgt tctcgatgcc
     2161 ctcctgcgcg aggcgcggcg cgtcggggtc gagattctcc acccctgccg cgtgacggcg
     2221 atcgaacgag ccagcgacgg attcgtcgtc acggcggggg cgcatcgcct cgaagccgag
     2281 cgcgtcgtga ttgcgacggg gggaaagagc attcccaagt cggggtcgga cggcctcggc
     2341 tacgaattgg tgaaagcgct gggacattcg atcacgccgc tcgttactcc gggcctggtc
     2401 ccgctgaccc tcccgcgcga ccactttctg tgcgcgctcg ccggtgtaac gctcgacgta
     2461 accatcgagc tgcgatccgg aaccggcaag cggctcgagt cgtttaccaa ctcgacgctg
     2521 ctcacccact tcggcctgtc gggaccgtcg gtgctcgata tcagccgcta ctacctgcag
     2581 gcgatcgcga gcgattccaa ggcgggtctg ttcgcgaact ggctcccggg cagggacgag
     2641 gacgcgctcg accgcgagct gcaatcgttg cggggaaaat ccgttggcgg ttacctgcgc
     2701 gagcggctac cggagcggtt ggcccgcgcg ctctgcgagc acgccggtgt tgatcccgcc
     2761 acccgcggcg acgccctcaa gcgcgacgga cgccgggcgg tcgccgccgc ggcgtgcaac
     2821 ctcccgctcc cggtcaccgg gaatcgcggc tacacctacg ccgaggtgac cgcgggcggt
     2881 gtgcccctgg cccaattgca tctggatacc atggagtccc gcgtgtgtcc gggcctctat
     2941 ctgtgcggtg aaatctgcga cgtcgacggg cgcatcggcg gcttcaactt ccagtgggcg
     3001 tggtcgagcg ggttcgtggc ggggacggcg gcgtcaaagt agcgtgtgac gattcgtcga
     3061 ctggtgtgtc tcacctcaac acaggaggca agggcaccat gtttcgtcga acgtccccct
     3121 cgaactggct ctttctatgg gcctccgcgc tcgtattcgc gggctccttg agctgcagcg
     3181 acgatccttc ggctcccgag gagcccgaag atcgtaacgt ctcggtcgaa tcctcgaacc
     3241 gcgagactgc cactatcggc tcggcgggcg ggtcggtatc gacgaccaac aacgccggga
     3301 tcacctacac gctcgacatt cccccgtatg ccctcccggg cgacgtcgaa atcgcgatca
     3361 ccccggtgac cgcgatcgac aacctcccct tcgacgggag actcgagggt gccgtacgcc
     3421 tgggcccgtc ggggttgcag ctgatcaagc cggccattct caccatcgcc caggccccgt
     3481 cggcgtcggg gaccgagcgc ctgatcggat tcgcctacca gggcgacgcc gattcgttcg
     3541 aacccgccgc cgcgatcgac ggcaccactg aaatccgtgt accgatcacg cacttcagcg
     3601 gcgccggcgc cgcgtccggc gagcaagaag agctcgaacc ctggatcagg caaatgtgcg
     3661 caagtattcc cactcgcgac cacacgttct acgagcaagt ggagttgtgc ttcgatggct
     3721 ctgtgacacg gcgcgactgg cttctcgacc tcgcacgtga ttacctcgac cagatcgtga
     3781 tcccgctgat ccataccgac ctgtcagatg gtgtcgcgca gtacatcggc tggtactcgg
     3841 gatgcaattt gaatgcgcag acattcaaca tactcgattg ggagaccaca ctcgcgaccg
     3901 agatcgagaa tgttcaggaa ttgctcgcca cagaactcgt cgccaccatc gccggagaga
     3961 agtccgcgct atgttcccaa ggcggaatcg agcacctgta ctggatcctc cactaccgat
     4021 atctcgccca ggctgccggg gccggggacg tggccggact tcgcgacgaa gacatcttag
     4081 atggcttgtg cgccgaggtc cgcatccccg cggttacgct ggcggatccc atgcccctga
     4141 accagggcac cagcctcgac gcacgcgccg aactttggat cagcaaccaa cgcatcgacc
     4201 tccccgtgtt ccggttcacc gtgaatccca cgaccgccaa cgcgacgtgt ggcaccgggc
     4261 agaccatctg cggcggcaac tcgaacgagc tcggcgaata cacggccgtg gtgacgcgca
     4321 ccgggccggg gcgggtcggg atcgacctgt ttgccgccct gctgatagcg actccgtcgg
     4381 gcaccgtcca cgcgctcgcc gaaacgccgc tcgtcggttc ggagacgatc gaacgcgaga
     4441 gctactcgat cgctgcgaca ttcccaacga cggttgagcc aggcacgccg gtgacactca
     4501 ccgcggtcgt cacgcgaaac aatgacgtcg gcagtcccca accggtcgaa ggcgcggtcg
     4561 tgacgttcga cgtcgacggc ggggtcgcgg acccagtcgg tggacagacg gacgccagcg
     4621 ggtcggtttc aacccaggtg accgccaacg acgggcccaa tcgcgtcgtc gtcgacatcg
     4681 cggctgcgtt cgacggccgt caggtcgcaa cgaagacagt cgaagccgcg atcgaaacga
     4741 ctgaacccac cggccgtctc gtattgctct cgcgagacac acaaattctc gcgtgggttc
     4801 cgaacccgga cgagtgcatg ggcgaccccg gtgcggatac acgctcgtcg gaactgggtc
     4861 gggtcgaggt cacggaggac gcaaacatgt cgtgctccaa cgcgaccggc accggtcacg
     4921 gacaggcagt gaaccagcat gtttcggacc ctggcttcgc agccgacggt agttccgcgc
     4981 tactgtactt ttttacggcg gggtcggcat ccgtgcaggc gaccggtgac tacgtatacg
     5041 cggcgtcggt gctgggcgaa atgctggtca gtttccaggt cttggaggga accgtcgcgt
     5101 acgagatcaa tgctgccttc aacggaacca tcggagatgt atccgtcacc ctgcaaggtg
     5161 gatcgacgtc ggtcaattgg acgaagaaac cgggcgacgg caatcccaac ccattcgcga
     5221 cctccggaac cttgtctccg ggtggctatg tcgcgagatt ccgtatcatt gctgagttgg
     5281 attctcaggt cgctcccgcg agcgggacga gcggtgtcac cgcgaccatt cgcctgaccg
     5341 cggctccggc agcagcggcg ggcaatccga ctccgctatc ccgttctccg cgcgatcgag
     5401 tcgtgcgatc gcgttgaagg gttattcgcc ggcgcgcccg gccaagagca gtcgccagtc
     5461 cgtgaccaag tgcagcgtga gggaactgcc gccctgcgga ac
//
LOCUS       DASPGK010000341         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1138390, whole genome shotgun sequence.
ACCESSION   DASPGK010000341 DASPGK010000000
VERSION     DASPGK010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138390"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VEC56_09545"
     CDS             complement(<1..717)
                     /locus_tag="VEC56_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HXV14436.1"
                     /translation="MRFRRTSPLVLLLCIASTASFAVTNGRGGGDGGVVLRANVPMGT
                     VLSTGDPVSFEYQTRKDAAVLVFNIDSEGYVHLLYPTAGPETSRALQTYTVPAEGAEL
                     VVDATTGVEFVFALAVDDPSAIDAAELDRLRGTDVEGAEPYRIDGDPFIAANMIAGEL
                     VRGVSHRGVYFGYTYFYVNERVEYPCYLCGACDGTPDDPACTDYRVVQNFDRRNPLVY
                     PLKRAYETVAGDAEVLADGGA"
     gene            992..1765
                     /locus_tag="VEC56_09550"
     CDS             992..1765
                     /locus_tag="VEC56_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV14437.1"
                     /translation="MDLFAAVPAAMGRSLPQILAESGRFGLFILALTFVMSVITWAVV
                     WDRARLYKKLRERGAALKRVVVQRGVPSAMQDADRYLPSIEASLLIEARHFLASRGAD
                     GVLMIDDPAEADGERSLLKGALEGRAMTEIGEMERHLNLLSTTSAGAPFLGLLGTVWG
                     IMHSFLSMGLEGAASIEVVGPGIAEALATTIAGLAAAIPALFAYNAFSRAVRRKESEL
                     DWFIARVLDGAVVSRGSQRATNPGGSGSPSRPARTGATY"
     gene            1779..2192
                     /locus_tag="VEC56_09555"
     CDS             1779..2192
                     /locus_tag="VEC56_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HXV14438.1"
                     /translation="MLKRTPYSNMADINVTSLVDVMMVLLIIFMLTAPFLQAGIEVKL
                     PKAKSTVIKETDAVVISITKDGKVFINDERVEPNQLSSTLATLKAAGEEVVFVRADQD
                     ATHGTVMSVIGEVKAAGINDVGMITEMKRDAKRKR"
     gene            2203..2898
                     /locus_tag="VEC56_09560"
     CDS             2203..2898
                     /locus_tag="VEC56_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV14439.1"
                     /translation="MRKGIVISLAAHALATLIVFVSVSFRQVTYLPRETYRVRLVTAA
                     VAEPARQTKPVVSEAPAPKETPKETPKEEPKDDELAAPIEKPKPKPKPKDEKSNEVPR
                     AEITKSDAGAAEGDTLAGETGEAAQGISFDGGDFPYDAFIARMRHKIAAAWQVPAGSE
                     GIERFAVVYFRVHRDGNITHVSVERGSGVFLFDQSCQRAVIQAAPFPPLPREYADEYV
                     GVHFSFKFVPAQP"
     gene            2943..>3773
                     /locus_tag="VEC56_09565"
     CDS             2943..>3773
                     /locus_tag="VEC56_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14440.1"
                     /translation="MTKLRWIVCLLWTALPVTAAAQTDVWPDPVVKEDVFVVPLWLPQ
                     FQGAGEHTPRAQRIEQIVLQDLQFSGLFKIMREEPATAGVAGINAVVVRGRVFGDGSD
                     VSFEGTVTDIGSGDFVGGKKYKVTDKETRRVAHLFADEIVRLVTGEKGVASTQIVYTR
                     KVGDKWELVVSDYDGYNPRVLVRQTVPLLAPRWIDRNQAVAYTSFRDGKADLFTRVLS
                     ESKSRGLANYSGSNTAAEWSDKQGLLLAALSKDGNTEIYMLARNGHLQQRLTHNKAID
                     T"
BASE COUNT          688 a         1174 c         1296 g          615 t
ORIGIN      
        1 cgcgccgccg tcggcgagca cctcggcgtc accggccacg gtctcgtatg cgcgcttgag
       61 cgggtacacc agcgggttac gacgatcgaa gttctggacc acgcggtaat ccgtgcacgc
      121 ggggtcgtcg ggcgtgccgt cgcaagcgcc gcacaggtag cacggatatt cgacgcgttc
      181 gttcacatag aaataggtgt atccgaaata cacgccgcgg tgcgacacac cgcgcaccag
      241 ttcgccggcg atcatgttgg cggcgatgaa cgggtcgccg tcgatgcgat acggttcggc
      301 gccctccaca tcggtaccgc gcagccggtc gagctcggcg gcgtctatcg cggacggatc
      361 gtccaccgcg agcgcgaaca cgaactcgac gccggtggtc gcgtcgacca ccaactccgc
      421 gccctcggcg ggcaccgtat aggtctgcag ggcccgcgac gtttcgggcc ccgcggtcgg
      481 atacaggagg tggacgtagc cctcggaatc gatgttgaac accagcaccg cggcgtcctt
      541 gcgcgtctgg tactcgaaag agacggggtc tccggtactc agcacggttc ccatggggac
      601 gttggcgcgc aggaccacac caccgtcgcc cccgccgcgg ccgttggtca ctgcgaagga
      661 cgcggtggaa gcgatgcaga gcaagaggac gaggggggaa gttcgacgga atctcatgac
      721 gctacctccc gttcagtgac gggcaagtcc ccggggtggc tcgattacga ggggctcttc
      781 gtggccagta aaatatagcc catcggctcc ggagctacaa gcacggggaa ccgagcccca
      841 ccctgggggt atgcacgaag cgggccgacg tggccccgcc gaacgggcac tttttctagg
      901 caaggtatgg cggttgtgct agaccttggc cgaaattcga cgcgatgtcc ctctcgcgtc
      961 gaaggcgaca ctcatcgagg aaggaatcca tatggacttg ttcgcggccg tgcccgcggc
     1021 gatgggacga tccttgccgc agatcctcgc cgaatcggga cgattcggtc tgttcattct
     1081 tgcgctcacg ttcgtgatgt cggtgatcac gtgggccgtg gtgtgggatc gtgcccgcct
     1141 gtacaagaag ctgcgcgaac gcggagccgc gctgaagcgc gtggtggtgc agcggggcgt
     1201 gccctcggcg atgcaggacg cggaccgcta cttgccgtcg atcgaagcct cgctcctgat
     1261 cgaggcgagg catttcctcg cgtcgcgcgg cgcggacggc gtgctcatga tcgacgaccc
     1321 ggcggaagcc gacggcgaac gctcgctctt gaagggcgcg ctcgaggggc gcgcgatgac
     1381 ggagatcggc gagatggagc gccatttgaa tctgctctcg acgacgtcgg cgggcgcgcc
     1441 gtttttggga ctgctcggca ccgtgtgggg aatcatgcac agcttcttga gcatggggct
     1501 cgagggtgcc gcgagcatcg aagtggtggg gcccggcatc gccgaggcgc tggcgaccac
     1561 catcgccggt ctggcggccg ccatccccgc actctttgcg tacaacgcct tcagccgcgc
     1621 ggtgcggcgc aaggaatccg aactcgactg gttcatcgcg cgcgtcctgg acggagccgt
     1681 ggtgtcgcgg ggctcgcaac gcgctactaa cccgggcggg tcgggcagtc cgtcgcgccc
     1741 ggcgcgcacc ggcgcaacct actgatcgtg agacgtgcat gctgaagcga acgccatact
     1801 ccaacatggc cgacatcaac gtgacgtcgc tggtcgacgt catgatggtg ctgctgatca
     1861 tcttcatgct caccgcgccg tttttgcagg cgggcatcga ggtcaagctg cccaaggcca
     1921 agtcgactgt gatcaaggaa accgacgcgg ttgtgatatc cattaccaaa gacggcaagg
     1981 tcttcatcaa tgatgagcgc gtcgagccga atcaactgtc gtcgacgctg gccacgctga
     2041 aagccgcggg cgaagaagtg gtgttcgttc gcgccgacca ggacgcgacg cacggcacgg
     2101 tgatgtccgt gatcggcgag gtcaaggcgg ccggcatcaa cgacgtcggc atgatcaccg
     2161 agatgaagcg cgacgcgaag cgcaagcggt gagcggcgcg ccatgcgcaa gggcatcgtc
     2221 atatcgctcg ccgcgcacgc gctcgcgacc ctgatcgtgt tcgtatcggt ctcgtttcgc
     2281 caggtgacgt acctcccgcg cgagacctat cgcgtgcgct tggtgacggc ggcggtggcc
     2341 gagccggcgc ggcaaaccaa gccggtggtt tccgaagccc cggctcccaa ggagaccccc
     2401 aaggagactc ccaaggagga acccaaggac gacgagctcg cggcgccgat cgagaagcca
     2461 aagcccaagc ccaaaccgaa ggacgagaag tccaacgagg tgccccgcgc cgagattacc
     2521 aagagcgacg cgggagccgc cgagggcgac acactggcgg gcgagaccgg cgaggccgcc
     2581 caggggattt cgttcgacgg cggcgatttt ccgtacgacg cgttcatcgc gcgtatgcgg
     2641 cacaagatcg cggcggcgtg gcaggtgccg gcgggcagcg aaggaatcga acgtttcgcg
     2701 gtggtctatt ttcgtgtgca ccgcgacggg aacatcaccc acgtgtcggt ggagcgcggg
     2761 tcgggtgtat tcctgttcga ccagtcgtgc cagcgcgcgg tgattcaggc cgctccgttt
     2821 ccgccgctgc cgcgcgagta cgccgacgag tacgtgggcg tgcattttag tttcaagttc
     2881 gtaccggcac agccgtgagc gcattcaggg tgcggcgtgc gaatcggaaa gggtatgtcg
     2941 acgtgacgaa gttacgctgg atcgtgtgcc tattatggac ggccctgccg gtcacggctg
     3001 ccgcgcagac cgacgtgtgg cccgaccccg tggtcaagga agatgtgttc gtcgtgccgc
     3061 tgtggctccc gcagtttcag ggcgccggcg agcacacgcc ccgcgcgcag cgcatcgagc
     3121 agatcgtcct gcaggacctg cagttctcgg ggctcttcaa gatcatgcgc gaggagcccg
     3181 cgaccgcggg tgtggccggt atcaacgcgg tagtcgtacg cgggcgggtc tttggagatg
     3241 gatccgacgt gagcttcgag ggcaccgtca ccgacatcgg gtccggcgac ttcgtgggcg
     3301 gcaagaagta caaggtgacc gacaaggaga cgcgccgcgt ggcacacctc ttcgcggacg
     3361 agatcgtgcg attggtcacg ggcgagaagg gcgtggcctc gacgcagatc gtctacacgc
     3421 gcaaggtcgg cgacaagtgg gagctcgtgg tctcggacta cgacggctac aacccgcgcg
     3481 tgctcgtgag acaaaccgtc ccgctcttgg cgccgcgctg gatcgacagg aaccaggcgg
     3541 tcgcgtacac gagcttccgc gacggcaagg cggacctgtt cacgcgcgtc ttgagcgaga
     3601 gcaagtcgcg cggtctggcc aactacagcg gcagcaacac ggcggccgag tggtcggaca
     3661 agcagggcct gctcttggcc gcgctatcaa aggacggcaa caccgagatc tacatgctgg
     3721 cgcgcaacgg gcacctccaa cagcgcctga cccacaacaa ggccatcgac acc
//
LOCUS       DASPGK010000342         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_787881, whole genome shotgun sequence.
ACCESSION   DASPGK010000342 DASPGK010000000
VERSION     DASPGK010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787881"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1627)
                     /locus_tag="VEC56_09570"
     CDS             complement(158..1627)
                     /locus_tag="VEC56_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXV14441.1"
                     /translation="MRLRQLCAFWLVAAVLSAATVFGAADIKRGPQPTPRASLTPRVG
                     DFDMMLERRVVRVLAPPSRTLYFNDKGRERGITAELVRDFEEFLNQKYRRKLHNRPIT
                     VFLIPTAREGLISDLLAGKGDIAAGNLTVTDERLREVDFIAPPDLKEVSELVLTSKRR
                     GAMASVDELSGQTVHVRASSSYHQSLQTLAARFQREGKPPVKLVPVPDMVEDEDLMEM
                     LDAGVIDVIVVDDWKAKMWAKILPNLIVNEGVAVRTGAHVGWAYRKNSPLLLAELEEF
                     YYKYEKQRGTIPYRFAQYNKQVKRLQDPTGRNDWKRFEETVALFEKYGAQYGFDPIML
                     AAQGFQESKLDQTVRSPVGAIGIMQVMPETGASLKVGDITVTEPNIHAGAKYMNTLMT
                     KYFQDAHFDELNRTLFAFASYNAGPNRIARLRKLAPERGFNPDVWFDNVEVIVSEKVG
                     RETTTYVRNIVKYYLSYRLTIQMNEEQRKAREALTPAGG"
     gene            complement(1633..2454)
                     /locus_tag="VEC56_09575"
     CDS             complement(1633..2454)
                     /locus_tag="VEC56_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXV14442.1"
                     /translation="MSNKRIRALRVLVTAGLTAVVLWSCGTVPITGRRQLNLVDDGQI
                     MAMAASQYDTVLMTSKLSTNKEQTAMIKRCGARIQKAVEAYFQSKGQMAQLAGYEWEF
                     NLIESDEMNAWCMPGGKVAFYTGILPVCKDETGVAVVMGHEVAHAVAKHGNERMSQAM
                     MAQYGSVLLDAAMAKNSTQTKQMAATAFGIGATYGALMPFGRKQESEADHLGLIFMAM
                     AGYDPKAAVPFWERMAAGGGGKPPEILSTHPSDATRIANLNKLMPEALTYYKPAN"
     gene            complement(2474..>4864)
                     /locus_tag="VEC56_09580"
     CDS             complement(2474..>4864)
                     /locus_tag="VEC56_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXV14443.1"
                     /translation="RELTEKEAEKAAIDLSYFAEIYLMRADGSEVKRLTHEPGYDGGP
                     FFRPDGKRIVWRRFSEDGAVADVFTMKPDGSDVVQITDFGAMSWAPFFHPSGEYLIFT
                     SNKLGFENFELYIVDARGEKEPVRITYTDGFDGLPVFSPDGKQLAWTSNRTSDKTSQI
                     FIAEWSHEAARAALGRGYSRMDHSGVDHRSGRSRAPLERSAASANVSFSPEIRAGDLK
                     NDVSVLASDEMEGRMTGSKGEKASADYIAKRFRDMGVMPFDEKVGYFQTFPFTAGVKV
                     VPRDNAMSLISGSAKRVLEVEKDFRPLAFSENATVEGPVAFAGYGLKTPGTSAYDSYG
                     DFDVKDKVVLVLAYVPEGVAMERRMELNMYAGARYKAMIAREKGAKALLFVTGPNSPN
                     AGVLSKLGFDQSMAGSGIPVVSISGTVADEMFKVHNQTLKEVQDALDVENPHAVGTFD
                     LTGVTVSLTTKIEREMGDGRNVIGYLPAFQKKDAPVIVIGAHYDHLGHGEDNSLARKG
                     EEGQIHNGADDNASGTSTMMEVAHALSDMRGRHPDLFLYDVVFAAWSGEELGIIGSNY
                     FVEHPPVAKERIAAYINFDMVGRIKDNTLIVQGVGSSEAWKGLVEKNNVPVGFSMKLD
                     DDPYLPTDVTAFYPKGVPVLAFFSGNHEDYHRPTDDVETLNYDDMARVAKLVTNLTLD
                     LMKREAPPAYVKVAPTSAHAGSRASMRVYLGTIPDYAPGDIEGLKLSGVKAGGPAEKA
                     GLQGGDVIVECAGKAIKNIYDYTYAMDALKIGEAVPIVVVRDGKRVTLTIVPEARQ"
BASE COUNT          773 a         1552 c         1571 g          968 t
ORIGIN      
        1 ctcatagaca aggcattgcc ggccggcgac cacacggcag cctgggacgg acgcgacgtg
       61 gatggtcgcg cgcagcccag cggtgtctac ttctgccgtc tcacggtcgg tgggacgccg
      121 gccggcacgc agcggctcgt cctggtgagg tgatcgtcta gcccccggcc ggtgtcagtg
      181 cctcgcgtgc cttgcgttgt tcctcgttca tctggatcgt gagccggtac gagaggtagt
      241 acttcacgat gttgcgcaca tacgtggtgg tctcgcgtcc cactttctcg gacacgatca
      301 cctcgacgtt gtcgaaccac acgtcgggat tgaatccgcg ctccggggcc agcttgcgca
      361 agcgggcgat gcggttcggg cccgcgttgt aggaagcgaa ggcgaacagc gtccggttca
      421 gctcgtcgaa gtgcgcgtcc tggaagtact tcgtcatgag cgtgttcatg tatttggcgc
      481 cggcgtggat gttgggctcg gtgaccgtga tgtcgcccac cttcaaggac gcgccggtct
      541 cgggcatgac ttgcatgatg ccgatcgcgc ccacgggact gcgcacggtc tgatcgagct
      601 tcgattcctg gaagccctgc gcagccagca tgatcgggtc gaagccgtac tgcgcgccgt
      661 acttctcgaa cagtgcgacc gtttcctcga atcgcttcca gtcgttacgt cccgtgggat
      721 cctgcaagcg cttgacctgc ttgttgtact gcgcgaagcg ataggggatc gtgccgcgct
      781 gcttctcgta cttgtaatag aactcctcga gctcggcgag cagcagcggg ctgttcttgc
      841 ggtaggccca gccgacgtgg gcgccggtgc gcacggcgac gccttcgttc acgatcaagt
      901 tggggagaat cttcgcccac atcttcgcct tccagtcgtc caccacgatg acgtcgatga
      961 cgccggcgtc cagcatctcc atgagatctt cgtcttccac catgtcgggg acgggaacga
     1021 gcttcaccgg cggcttgcct tcccgttgga agcgcgcggc gagcgtttgc aggctctggt
     1081 ggtagctgga cgacgcgcgc acgtgcacgg tctggcccga cagttcgtcg accgacgcca
     1141 tcgcgccgcg ccgcttgctg gtcaatacga gctccgagac ttccttcagg tcgggtggag
     1201 cgatgaaatc cacctcgcgc aggcgttcat cggtgaccgt gaggttacct gcggcgatgt
     1261 cgcccttgcc cgccagcaaa tcggagatca ggccttcgcg cgcagtagga atgagaaaca
     1321 cggtgatggg ccggttgtgg agcttgcggc ggtacttttg attcaggaat tcctcgaagt
     1381 ctcgcaccag ttcggcggtg atcccgcgct cgcgaccctt gtcgttgaaa tagagcgtgc
     1441 ggctgggcgg ggcgagcacg cgcacgacgc ggcgctcgag catcatgtca aagtcgccca
     1501 cccgcggcgt gagcgacgcg cggggcgtgg gttgggggcc gcgcttgata tccgccgcgc
     1561 cgaacaccgt tgccgccgac agcacggcgg cgaccagcca gaaagcgcag agctgacgaa
     1621 gtcgcatagg tgctagttcg ccggcttgta atacgtcaac gcctccggca tgagcttatt
     1681 gagattggcg atgcgggtcg cgtcggaggg gtgcgtgctc aggatctcgg gcggcttacc
     1741 gccgcctccg gccgccatgc gctcccagaa cggcaccgcg gccttggggt cgtagccggc
     1801 catggccatg aatatcaagc ccaggtggtc ggcttcactc tcttgtttgc gaccgaacgg
     1861 catcagcgct ccatacgtgg cgccgattcc gaacgcggtc gcggccattt gcttggtctg
     1921 ggtggagttc ttcgccatgg cggcgtcgag gagcacgctc ccgtactgcg ccatcatcgc
     1981 ttggctcata cgctcgttgc cgtgcttggc taccgcgtgc gcgacttcgt gacccatcac
     2041 gacggcgacg cccgtctcgt ccttgcacac gggcagaatg ccggtataga acgcgacctt
     2101 gccgcccggc atgcaccacg cgttcatctc gtcgctctcg atcaggttga actcccattc
     2161 atagcccgcg agctgcgcca tctggccctt ggactggaag tacgcctcga ccgccttctg
     2221 gatgcgcgcg ccgcagcgct tgatcatcgc ggtctgctct ttgttggtcg agagcttgct
     2281 ggtcatgagc acggtgtcgt actgcgacgc cgccatggcc atgatctggc cgtcgtcgac
     2341 gagattcaat tggcggcgcc ccgtgatcgg gacggtaccg cacgaccaga gtacgacggc
     2401 cgtcaggccg gcggtgacca acacccggag tgctcgaatc cgcttgtttg acattgttct
     2461 tatctcctcc cgattactgc cgtgcctcgg ggacgatggt gagcgtcacg cgcttgccgt
     2521 cgcgtaccac cacgatgggc accgcttcgc cgatcttgag cgcgtccatg gcgtaggtgt
     2581 agtcgtagat gttcttgatc gccttgccgg cgcactcgac gatcacgtcg ccgccttgca
     2641 ggcccgcctt ctcggccggg ccgccggcct tgacgccgga cagcttcaag ccctcgatgt
     2701 cgcccggcgc gtagtcgggg atggtgccga gatagacgcg catcgacgcg cgcgatcccg
     2761 cgtgcgccga ggtgggcgcg accttgacgt aggccggggg tgcctcgcgc ttcatgaggt
     2821 cgagcgtcaa gttggtgacg agcttcgcca cccgcgccat gtcgtcgtag ttgagcgtct
     2881 cgacgtcgtc ggtggggcgg tggtagtcct cgtgatttcc ggagaagaac gcgagcacgg
     2941 gaacgccctt gggatagaac gcggtcacgt cagtgggcag ataggggtcg tcatcgagct
     3001 tcatgctgaa tccgaccggc acgttgtttt tctcgaccag gcctttccac gcttccgacg
     3061 accccacgcc ctgaacgatc agcgtgttgt ccttgatgcg cccgaccatg tcgaagttga
     3121 tgtacgcggc gatgcgctcc ttggccaccg gcgggtgctc gacgaaatag ttcgatccga
     3181 tgatgcccag ctcttcgccc gaccaggccg cgaagacgac gtcgtagagg aaaaggtcgg
     3241 ggtggcgtcc gcgcatgtcc gaaagggcgt gtgcgacttc catcatggtc gacgttcccg
     3301 aggcgttgtc gtcggcgccg ttgtgaatct gaccctcttc gcccttgcgc gcgagtgaat
     3361 tgtcctcgcc gtggcccagg tgatcgtagt gcgccccgat cacgatcacg ggtgcatcct
     3421 tcttctggaa cgcggggagg tagccgatca cgttgcgccc gtcgcccatc tcgcgctcga
     3481 tcttggtcgt gagcgatacc gtgacgccgg tgagatcgaa cgtgccgacc gcgtgcgggt
     3541 tctcgacgtc cagcgcgtct tgcacctctt tgagcgtctg gttgtggact ttgaacatct
     3601 cgtccgcgac cgtaccgctg atcgaaacca ccggaatccc cgagcccgcc atcgattggt
     3661 cgaagccgag cttcgagagc acgcccgcgt tgggcgagtt gggcccggtg acgaacaaga
     3721 gcgccttggc gcccttctcg cgcgcgatca tcgccttgta gcgcgcgccc gcgtacatat
     3781 tgagctccat gcggcgctcc atggcgacgc cctcgggcac gtaggccagc acgagcacga
     3841 ccttgtcctt gacgtcgaag tcgccgtagg agtcgtacgc gctcgtcccg ggcgtcttta
     3901 gaccgtaacc cgcgaacgcg accgggcctt cgacggtcgc gttctcgctg aacgcgagcg
     3961 ggcggaagtc cttctcgacc tcgagcacac gcttcgccga gccggaaatg agcgacattg
     4021 cgttgtcgcg ggggacgact ttgacgccgg cggtgaacgg aaaggtctgg aagtacccga
     4081 ccttctcgtc gaagggcatc acgcccatgt cgcgaaagcg cttggcgatg tagtccgcgg
     4141 acgccttctc gcccttcgag ccggtcatgc gtccctccat ctcgtcggac gccagcacgg
     4201 agacgtcgtt cttcaagtcg cccgcgcgaa tctcgggcga aaaggaaaca ttcgcgctcg
     4261 cggccgagcg ttcgaggggc gcgcgcgaac gcccgctcct atggtcgacg ccgctgtggt
     4321 ccatccgcga atagccgcgc ccgagcgccg cgcgcgccgc ctcgtggctc cactccgcga
     4381 tgaagatctg cgaggtcttg tcgctggtac gattcgacgt ccacgccagc tgcttgccgt
     4441 cgggagagaa cacgggaagc ccgtcgaaac cgtcggtgta cgtgatgcgc accggttcct
     4501 tctcgccgcg cgcatcgacg atgtacagct cgaagttctc gaaaccgagc ttgttcgacg
     4561 tgaagatcaa gtactcgccg ctagggtgga agaacggagc ccacgacatg gcgccgaagt
     4621 cggtgatttg gacgacgtcg ctgccgtcgg gcttcatcgt gaacacgtcc gcgaccgcgc
     4681 cgtcctcgct gaagcggcgc cacacgatgc gcttgccgtc gggcctgaag aaggggccgc
     4741 cgtcgtagcc gggctcgtgg gtcaagcgct tgacctcgga gccgtcggcg cgcatcaggt
     4801 agatctccgc gaaataggag agatcgatcg ccgctttctc cgcttccttc tcggtgagct
     4861 cgcg
//
LOCUS       DASPGK010000343         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1371787, whole genome shotgun sequence.
ACCESSION   DASPGK010000343 DASPGK010000000
VERSION     DASPGK010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371787"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VEC56_09585"
     CDS             <1..938
                     /locus_tag="VEC56_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14444.1"
                     /translation="QRATTTSKPESRITPALAVETVTPAPEVATTPTPVITDVTYEQS
                     ETAFLEGRYDEAVDLFTAYTQRRESNPWGHYMLGMSAWKSGRLETAEAAFKRTLELDS
                     TLVKAHLNLGRVLIEASRPSEALAAIERAIAVEGETSVAYRLKGNAYHTLGQNEDALA
                     SYRRAIQIDPEDAWSMNNLAFILIEAGRYDEALPALARATELRKDVAVFFNNLGMALE
                     CTGHFRASEEAYTLASSLDGVSAKSVANRDRVAAVLEQPGTPAVDLAALARTFESEIA
                     GSQAATVANATTIDAHAGAVPVPTRGDSAVSGDRP"
     gene            1139..>2755
                     /locus_tag="VEC56_09590"
     CDS             1139..>2755
                     /locus_tag="VEC56_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14445.1"
                     /translation="MNALRAVLLGPLVMACLIACDDNPVDDDTGNPPDPTKADQVATI
                     GFQALGDTLAALENRDLDQLRGVRFDAIRDKLEEALTHESNNGIAHLGLAIVEIVELN
                     YSDEIWGVVDSLDAWQDAGGDIDWPPFALTPRDPHRMLIGRQFELMTTLPVVMSMRMA
                     TEFPSNVSVAQIQAIISNEIRPALNRAVSHLNVVESETDTELRIHIDDNGVQEDIVVD
                     LGEILVFSAAVHAVRAGFSTALAYDVDFFGPDGGYDWLDDVSDLNGFTWCRDNGVSVT
                     VTDKGGGHVDLHFHGDQRRSDALTDSILVAVLHYNLEDREEFLALRDGGDALEDAHAD
                     LLGVLQRLESAAVFIRNRPHETEDNVIKLADLTDLDADVADPDRPNFMQGWTRIEDAI
                     DFVRDVLTGPVTFTEELGPNDTSFTFTVDLSRLFLSPTDDWNDLLPYHRWSLPSGGWL
                     TFTEETYSWDNGGFWHEEYYVRTGPSTCTYMYWDQVDMVTYTNRYWDLDGSVFDYLDA
                     PNGSPIDLDIERVPYFPDYTFDGIFPGMTRT"
BASE COUNT          512 a          914 c          876 g          453 t
ORIGIN      
        1 gccaacgcgc gacgaccacg tcgaagccgg agtcgcggat cacaccggcg ctcgccgtcg
       61 agacggtgac gcccgcgccg gaagtcgcaa ccacgccgac gcccgtgatc accgacgtca
      121 cctatgagca atcggagacc gcgttcctcg aaggccgcta cgacgaggcg gtcgacttgt
      181 tcaccgccta cacgcagcga cgcgagtcga atccgtgggg ccactacatg ctggggatgt
      241 cggcgtggaa gtcggggcgt ctcgagacgg cggaagcggc attcaagcgc acgctcgaac
      301 tcgactcgac tctcgtcaaa gcccacctca acctgggtcg cgtgctgatc gaagccagcc
      361 gtccgagcga agcgctcgcg gccatcgagc gcgccatcgc ggtcgagggc gagaccagcg
      421 tggcgtaccg cctcaaggga aacgcgtacc acacgctcgg tcagaacgag gacgcgctgg
      481 cgtcgtaccg gcgcgcgatc cagatcgacc ccgaagacgc gtggtcgatg aacaacctcg
      541 cgttcatcct gatcgaagcc ggccgctacg acgaggcact gccggcgctc gcgcgcgcga
      601 ccgagctgcg caaggacgtc gcggtgttct tcaacaatct cggcatggcg ctcgagtgca
      661 cgggacattt ccgcgcctcc gaggaagcgt acacgctggc gtcgtcgctg gacggggtca
      721 gcgcgaagtc ggtcgcgaac cgcgaccgcg tggccgccgt actcgagcag ccgggcaccc
      781 cggcggtcga tctggccgcg ctcgcgcgta cgttcgagtc ggagatcgca ggctcgcagg
      841 ccgcaacggt cgccaacgcg acgacgatcg acgcgcacgc gggtgccgtg ccggtgccga
      901 cacgcggcga ttccgccgtc tccggcgatc ggccgtaggg cgcgtgcgcg ccgaagggag
      961 ttggatcgcc ccgttccgcg agcgcggagc ggggcggtcg cgttcccgag ccccttgtaa
     1021 ccggttggtc gattagggta tcctgaacgg caaacgtggc ggcggtgcga caccatcccg
     1081 gaccaaccat cttcgccccg cgtttcttcc ccaacggtgc aggtggaagg agagccccgt
     1141 gaacgcgcta cgcgcggtcc tattgggacc gctggttatg gcgtgcctta ttgcctgcga
     1201 cgacaacccc gtcgacgacg acaccggtaa cccgcccgac cccaccaagg ccgaccaggt
     1261 tgcgaccatc ggattccagg ccctcggcga tacgctcgcg gcgctcgaga atcgggattt
     1321 ggaccaactc cgaggcgttc gcttcgacgc catccgcgac aagctcgagg aagcgctgac
     1381 ccacgagtcc aacaacggca tcgcgcacct cgggctggcg atcgtcgaga tcgtcgagct
     1441 caactacagc gacgagatct ggggtgtggt cgatagcctc gacgcctggc aggacgccgg
     1501 cggagacatc gactggccgc cgttcgcgct gaccccgcgc gacccgcacc gcatgctcat
     1561 cggtcgccag ttcgagctca tgaccacact acccgtcgtc atgtcgatgc ggatggcgac
     1621 ggagtttccc tccaacgtgt ccgtcgcaca gatccaggcg atcatctcga acgagattcg
     1681 tcccgccctg aaccgcgccg tgtcgcacct caatgtggtc gagagcgaaa ccgacaccga
     1741 gcttcgcatt cacatcgacg acaatggcgt tcaagaggac atcgtggtcg atctcggcga
     1801 gatcctggtc ttcagcgccg cggtacacgc cgtgcgggct ggattcagca ccgcgctcgc
     1861 gtacgacgtc gatttcttcg ggcccgacgg cggctacgac tggctcgacg atgtgagcga
     1921 cctgaatggt ttcacttggt gcagggacaa cggcgtgtcg gtcacggtca ccgacaaagg
     1981 gggaggtcac gtcgacctcc acttccatgg cgatcagagg cgcagcgatg ccctaacgga
     2041 ctcgatcctg gttgcggtcc tgcattacaa cctggaggac cgcgaggagt ttctggccct
     2101 tcgcgacggg ggcgacgcgc tggaggacgc ccacgccgac ctcctcggag tcctgcagcg
     2161 cctggagtcc gcggctgtct tcattcgtaa ccggccccac gagaccgaag acaacgtcat
     2221 caagctggcg gacctgaccg acctggacgc ggacgtcgcc gaccccgatc gacccaactt
     2281 catgcagggc tggacgcgca tcgaagacgc gatcgacttt gtgcgcgatg tgctcacggg
     2341 cccggtgacg ttcaccgaag agctggggcc gaatgacacc agcttcacct ttacagtgga
     2401 cttgagccgg ttgtttctct ctcctacgga cgattggaac gacctgctcc catatcatcg
     2461 gtggagtctc ccgtccggag ggtggctcac gttcaccgag gagacctact cgtgggacaa
     2521 cgggggcttc tggcacgagg aatactatgt gcgcacggga cccagcacgt gcacgtacat
     2581 gtattgggac caagtggata tggttacgta caccaaccgc tactgggact tggatggctc
     2641 ggtcttcgat tacctggatg cacccaacgg cagtccgatc gacctcgata tcgagagagt
     2701 gccgtacttc ccggactaca cgttcgatgg gatctttccg ggcatgacac gaacc
//
LOCUS       DASPGK010000344         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_436887, whole genome shotgun sequence.
ACCESSION   DASPGK010000344 DASPGK010000000
VERSION     DASPGK010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436887"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VEC56_09595"
     CDS             <1..703
                     /locus_tag="VEC56_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV14446.1"
                     /translation="NEYVLHGSKMWITNGVGSGVAVVWAKLDGEITGFLVERERKGFE
                     ARPVKYKLSMRASDTAELILDEVRIPKANRLPEANGLKCALMCLNQARYGIAWGVIGA
                     AMACFHESLEYSKDRVMFAKPIAGYQLTQKKLADMATDITLMQLLCLQLGRLKDEGKL
                     KFPHVSMAKRNNVRKALEIARTARSILGANGISGEYQSMRHMCNLETVDTYEGTYEIH
                     TLIVGHELTGIPAYE"
     gene            719..1930
                     /locus_tag="VEC56_09600"
     CDS             719..1930
                     /locus_tag="VEC56_09600"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV14447.1"
                     /translation="MSKKRVVILGGARTPMAEWVGGKRGDGEPGGALNSVSALDLGAV
                     AARGALAKSKVPAERIGHVVMGNALQTSGDAIYGARHVGLKAGVPIRVPALTVNRLCG
                     SGIQSVINGAHIIGEGEDYVLAGGMESMSQAPHVIRGARSGFKLGQGQLEDSLLVALK
                     DSYCGLLMAQTAEKLAKRMGISREEQDTYALRSTHLGEKAATSGVFAEEIVPVEIKRG
                     PKSTSVDKDDHFFVRANLEGLAKLKPAFGEDGTVTAGNASGMVDGAACLVIADGDAAA
                     RDGLTPMAEIVAWHVVGVEPSEMGIGPAPAIRGLLEKTGLALSKIDLFEINEAFAAQY
                     LAVEKDLGIDRDKVNVNGGAIALGHPLGATGTRLLLTLALELGRRRKTYGVASACIGG
                     GQGIAMLIQRV"
BASE COUNT          428 a          672 c          778 g          336 t
ORIGIN      
        1 aaacgaatac gtgctccacg ggagcaagat gtggatcacc aacggcgtcg gatccggtgt
       61 ggccgtggtg tgggccaagc tggacggtga aatcaccggc ttcttggtcg agcgcgagcg
      121 caagggcttc gaggcgcggc ccgtgaagta caagctctcg atgcgcgcca gcgacaccgc
      181 cgagctgatc ctcgacgaag tgcgcatccc caaggccaac cgtctgcccg aggcgaacgg
      241 gctcaagtgc gcgctcatgt gcttgaacca ggcgcgctac ggaatcgcct ggggcgtcat
      301 cggcgccgcc atggcgtgct tccacgagtc gctcgagtac agcaaggacc gcgtgatgtt
      361 cgccaagccc atcgccggct accaactgac ccaaaaaaag ctcgccgaca tggccaccga
      421 catcacgttg atgcagctct tgtgtttgca gttgggccgg ctcaaggacg aaggcaaact
      481 caagttcccg cacgtctcga tggccaagcg caacaacgtg cgcaaggcgc tcgagatcgc
      541 gcgtaccgcg cgttcgattc tgggtgccaa cggaatctcc ggcgaatacc agtcgatgcg
      601 ccacatgtgt aatctcgaga ccgtcgacac gtacgaaggc acctacgaga ttcatacctt
      661 gatcgtcgga cacgagctga ccggtatacc ggcgtacgaa tagtcgaaag gaatgggaat
      721 gtcgaagaag cgagtggtga tcctgggcgg cgcgcgcacg ccgatggcgg aatgggtggg
      781 tggaaagcgc ggcgacggcg agcccggcgg cgcgctcaat agcgtttccg cgctcgacct
      841 gggcgcggtg gcggcgcggg gcgcgctcgc caagagcaag gtgccggccg agcgcatcgg
      901 ccacgtggtc atgggcaacg cgctccagac cagcggcgac gccatctacg gcgcgcgcca
      961 cgtcggactc aaggccggcg tccccatccg cgtgcccgcg ttgacggtga accggctgtg
     1021 cggttcgggc atccaatcgg tgatcaacgg cgcgcacatc atcggcgagg gcgaggacta
     1081 cgtgctcgcg ggtgggatgg agagcatgag ccaggcgccg cacgtgattc gcggcgcgcg
     1141 ttcgggtttc aagctgggcc agggccagct cgaggactcc ttgttggtgg ctctcaagga
     1201 ttcgtattgc ggtctcttga tggcgcaaac cgccgagaag ctcgccaagc gcatgggcat
     1261 atcgagagaa gaacaggaca cctacgcgtt gcgcagcacg cacctgggcg agaaggccgc
     1321 gacgagcggc gtcttcgccg aggagatcgt gccggtcgag atcaagcgcg gacccaagtc
     1381 gaccagtgtc gacaaggacg accacttctt cgtgcgcgcg aacttggaag gactggccaa
     1441 gctcaagccc gccttcggcg aggacggcac cgtcacggcc gggaacgcga gcggcatggt
     1501 ggacggtgcg gcgtgcctcg tgatcgcgga cggcgacgcg gcagcgcgcg acgggttgac
     1561 gcccatggcg gagatcgtgg cctggcacgt ggtcggggtg gaaccgtcgg agatgggcat
     1621 cgggcctgcg cccgcgatcc gcggcctgct cgaaaagacc gggctcgcgc tctccaagat
     1681 cgacttgttc gagatcaacg aggccttcgc cgcgcagtac ctcgcggtcg aaaaggatct
     1741 cggtatcgac cgcgacaagg tcaacgtcaa cgggggcgcg atcgcgctcg gccacccgct
     1801 gggtgcgacg ggcacgcgcc tcctcttgac cctcgcgctc gagctcgggc ggcggcgcaa
     1861 gacctacggc gtcgcgagcg cgtgcatcgg cgggggacag ggcatcgcga tgctgattca
     1921 gcgggtgtag gtgcggggcg agcgtcgacg gttcccgacg cgagcttcct cagcgagcca
     1981 gaattaacga tcagatgcga gcgaggacct gagagcggag ggaagccttc gacgcgagcc
     2041 aggacccgct ccgtaggttc ggtgtagaag gaaggaagac gatggcaacg atagcagtga
     2101 ttcccggaga cggcgtcggc cccgaagtga tcgacgaggc gttgcgcgtg atgcgccgcg
     2161 tgagcgaaat cgacggcatt caattcgaca cggtgccgta cccgttcggc gccg
//
LOCUS       DASPGK010000345        17063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1020862, whole genome shotgun sequence.
ACCESSION   DASPGK010000345 DASPGK010000000
VERSION     DASPGK010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 17063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17063
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020862"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1114
                     /locus_tag="VEC56_09605"
     CDS             173..1114
                     /locus_tag="VEC56_09605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14448.1"
                     /translation="MKARTAVLLPCYNEAVTIGQVVADFRAALPDAVVYVFDNASDDD
                     TAAKARAAGAVIVPSPRRGKGNVVRHMFRTVDADLYVMADGDGTYPAHAAPELIRALE
                     QTDADMVVGTRLQHSAPKSFRSLHGLGNRVISGLISSLFSASLTDVLSGYRVFRRHFV
                     RTLYLRSSGFEIETEITLQALVKERDILEVPVPYGARPEGSHSKLRTWSDGALVLKSI
                     LLIFRDYKPLAFFSCVAGVCFVLGLLAGWLPVKDYIETRFVSHVPLALLAAALEVLAV
                     LFQGIGLILNAIKRFHQENQEMMSAILKRFDQDHEGP"
     gene            1101..1835
                     /locus_tag="VEC56_09610"
     CDS             1101..1835
                     /locus_tag="VEC56_09610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14449.1"
                     /translation="MKAPDDDARGTPYAGIDNLEVLAHAVRYNRFLVDTIVGAAQERG
                     KALDFGAGTGSLSTMVRDRGFEVACVETDARLRERLRSQGFRVYDDVALVPDRSYAFI
                     YTVNVLEHIEDDAGTLRVLLSKLEPGGRCLVYVPALGMLYSSMDRKIGHYRRYHRAGF
                     VRVATQAGFAVERAEYADSLGFFATLVYKLVGSRQGDLSTASVRLYDRFVFPMSRVFD
                     RMGCSRVLGKNLVAVIRRPGPTGERA"
     gene            1832..3442
                     /locus_tag="VEC56_09615"
     CDS             1832..3442
                     /locus_tag="VEC56_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14450.1"
                     /translation="MTAAKRIYLAAFALGFIACGTVSVLMGQDNNWDLRNYHCYNAYA
                     FLTDRLGFDYAPAQRQSYLNPFLDLPFYLGTIHLDPKLLGFLLGGIHGLSLGLLFAIC
                     MIVFRDHSAKARMVLSALCTGAGLYGPVFLGELGASQNDVLVGLFVLTALLLLVRTLA
                     VQQSLAPRAARRALVVASLVFGAGAGLKATLMPHAVGLAVAACAVEHTWRNRALVLAL
                     ACGPFLAGFLSSNGYWMLRLWQEFGNPVFPFYNDVFRSPWADPRSYADRSMIPLSMWD
                     AVRLPFAFVRDSDYTSLQNGFRDTRYAALYGVLVVYAVLWLGRRFVPRRAGRIEWRAM
                     AIESRFLLTFFVVSFVAWEAMFSIIRYTPTLELLAPLLLVVVGSHLLGHGVARTAVVA
                     TATLALLVATVKPIQHERLEWGETFWEIEVPPLPNPERTIVLIANARPLAYLIPAFPP
                     EVRWLSLENNLTEPSDQTRMQADMRRLITGHDGDVYLLSRAEPSPLLAHDVAVAGYYG
                     LRLESNTGLPIVSRHSPPGMFLWRLERY"
     gene            complement(3472..6048)
                     /locus_tag="VEC56_09620"
     CDS             complement(3472..6048)
                     /locus_tag="VEC56_09620"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HXV14451.1"
                     /translation="MRRALRYVLALAVPALLTSSPSSAQPVLTDSLYLAHHPRVLFTP
                     AELPVLRARIHDGGRDDEAYTYVRNYVQNIYSVLPPTTVLGPYFGLDAMPNLGLVAHL
                     ELPPNTAAMAQGKLLTLYLVNTYEPDYDEANSGLRLRSLALGYDLFFADATEAERALV
                     RDEMVRYIEPMIGTTAYESFEYRPYLGNHSAMFGAALGLAAISLQGEAEAEQLAAALA
                     MADRIAENLLTYQFDPGGAYNEGVLYALWTLRNLVYYFDARERFDGYSYADHATVRAV
                     EQWLPYELLPEGGGYSHNLNDSPYLTTPFSRSTTYVDWATSKWNSGLAAWLWEHSAGT
                     YGTDVGIMADKAGTILWHTNVPPIQPDDILPLHRVWVQRGLYHFRTGWQTTKTTIDDV
                     VFTFHSGKFQGGHAQEDQNTFALYAYGAKFAIDHGAGGLGKESEAHNLVLVDGLGQHN
                     AGSSIGTDGRIAEYLLGGSADYVVGDATQAYATYSEFNAPGVPFPGVDWSWGYSGANP
                     VQHAFRRVLVVHGEASSPYIVVMDDIDKDGAPHLYQWRMHTHFGNTVNTAASPWTIEG
                     AGAAMDVHLLHPSAESATVTTSAFENGNTDPNSTILRVTHTAIDPRFALMLVPRRGGS
                     PSPAVTREPYPWGYACSVDWGGGVVDRLVRNDAGAAVAHENIETDALVAWVREENGVV
                     AAYLAAGARSLVVGTNALVTVFDGPVTCEMSGSTIHIDRADAVFRFFDSGIAEVLCRE
                     QPVEFVLVGGYLVTAAATPVPNVPPPTAWTLAAHPNPFNSRTTLTYTVPADGPVTVAV
                     FDARGARVATLVHREFRRTGDHRVEWDGRTDGNRSAASGVYFARVEQNGAYRASKMVL
                     LK"
     gene            complement(6140..6646)
                     /locus_tag="VEC56_09625"
     CDS             complement(6140..6646)
                     /locus_tag="VEC56_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14452.1"
                     /translation="MRKHGLKAIAIAAVLLSGWLGAANAESSIGGGVHYLRNLGDIQT
                     YGYEENSFSLMASYQFAGPLLKFEADLEYVFDFAGTGEAMWEPQAYALVGGMIYGGAG
                     IGIGYLDGDWQNDPFYALRAGVNLPLGGFNLDMFASYRFQSDEELENLTGDDLDSMTF
                     GALMRFGL"
     gene            complement(6755..7678)
                     /locus_tag="VEC56_09630"
     CDS             complement(6755..7678)
                     /locus_tag="VEC56_09630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV14453.1"
                     /translation="MTILQAIGNTSMVRLRKVVPPHCGEIYVKLEWENPTGSMKDRMA
                     SAVISRAEEDGRLKPGYTVIEYTGGSTGASLALVCAAKGYRIHIVTSDAFSPEKRDQM
                     RALGAELTLVPSEGGLTTKKLILDMIEKARELGREPRTYLTDQLNNQDSITGYHALGE
                     EIWSQTGGEVDAFVHCVGTAASSRGVATVLKRHKPNVKLIVVEPEESAVLGGGAPGPH
                     KIEGVGIGYTPPLFDPSLVDDIIPIKTDDAKEMTRRLAREEALFAGTSSGANVLAAIQ
                     VAERLGPASKVVTLMCDSGLKYLNTDVYRSK"
     gene            complement(7732..8802)
                     /locus_tag="VEC56_09635"
     CDS             complement(7732..8802)
                     /locus_tag="VEC56_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV14454.1"
                     /translation="MKANLRNARALAMALRAAVARSSTVRVAATLDGGEGWRIGPRTL
                     PPPAGASEPLRKILTDMPAPSALLAPTTMAAWKAFIALGDAAKIELGDAFIKEHNLKV
                     AEERMSGVRVFRVTPPRIAPEHAHRLFVHVHGGGFIVGNGRAALPEALQIAESLKIPA
                     LTIDYRMPPDHPAPASMDDVVAVWRELVEAHSPATIILGGTSAGATLTLVATLRMKEL
                     GLALPAALFVGTPAADLAKRGDARFINDGIDHTLVSWDDVKAAIALYVGDKSYDDPHI
                     SPIFGDFTGFPPTYLISGTRDLLLSDVVRTHRKLRRAGVEADLNVYEGVAHADYSQLA
                     NTPECAEHYAELNAFVSRHLAR"
     gene            complement(8936..9370)
                     /locus_tag="VEC56_09640"
     CDS             complement(8936..9370)
                     /locus_tag="VEC56_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXV14455.1"
                     /translation="MKTKAKNGATKRTTSKPASKRIEKRIRDDGDWREETLARMRALI
                     LEADPEMIEERRWKKPSNPAGVPVWSHHGFVCTGEMYTKVVKLTFARGASLPDPKRLF
                     NSSLEGNARRAIDIREGEKVDARAFKALVKVAVAQNASMKKR"
     gene            complement(9403..10518)
                     /locus_tag="VEC56_09645"
     CDS             complement(9403..10518)
                     /locus_tag="VEC56_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14456.1"
                     /translation="MVRKLPNVVILLMVILLPVFTGCSSDSSTAPDGEEGPAITRVWI
                     EGSLGLVAGNSGQMQAYVLLEDGTTHDVTNEATWETLDPSKATVNQGMVTTIEAGRSD
                     LRIRYRGTTFTDPFFVYTRAPGVGEVKIEGGDLNNIIQFSVRGETRQLVATVRLPDAT
                     DMDVSDRVLWEASNPEAISVSDFGIATVNVSTGASELYVHWRGRYGSAVLQVGAPLPK
                     EYQITVEPFRIYAAYSCDSATNEDGGDGEFSYEINIVLPTGTRVPVAGTVDYPSEYYV
                     VIVDQNANYNLPTDQTSITLTDIQNLEVEVRLTEWDLEFVDFGDPVPDGDMDDQSDTK
                     VYRGSAGFSPGLHTITLAGPSGDCEIEISYYVIAVEN"
     gene            complement(10696..11127)
                     /locus_tag="VEC56_09650"
     CDS             complement(10696..11127)
                     /locus_tag="VEC56_09650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXV14457.1"
                     /translation="MKEPVHPLIAKVLSSKVPIAELIGFRVEEMTGGRAVGSMQSGPQ
                     HANPMGTLHGGVLCDLADAAMGMAFVSTLAPDESFTTIELNISFFRPVWQTVLRAEAR
                     VVNRGKSVGYVECEVTDGDGKHVAKASSTCFVLRGEQAKVR"
     gene            complement(11174..11623)
                     /locus_tag="VEC56_09655"
     CDS             complement(11174..11623)
                     /locus_tag="VEC56_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14458.1"
                     /translation="MAPVAYGALATVAGTVVISVMVGVIIGTAVVRLNNGLAWGFLAV
                     GIYLLTAPSMMDSPELPFASLNASPLILTFLSSSLAGRFFGIHRKSSAVGATLLALGS
                     GLVIGAAYLLLFRALFLTNRWTPPLVALVADVCLAAAALRVRRFRVP"
     gene            12148..13650
                     /locus_tag="VEC56_09660"
     CDS             12148..13650
                     /locus_tag="VEC56_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXV14459.1"
                     /translation="MYKLVLTDHSDAWTPGGLINVDVKYGMSSKFSLGVEGSWMKTYA
                     ADLSAKPGDGAGSSFDKIDGGPQQRGTVIGLIGEYQFSEDGTWAPYFDVGAGMYFWKW
                     TDADGNTLLSDDAALDDPLGGLYAVPDADQAGNPYELKDQELYLMAGLGIDYYASDLI
                     SIGLGVKFRYLTRVFTDFTDDKDIVGSDPGQLDLPKGIVEGLLGLTFHFGGKCPEMSG
                     TGSPSATTGIFPMDVQFESSATGGCPEYTYAWNFGDGTTSTEANPSHTYAQMGTYSPS
                     LTITDAKGNSALAAVPSITVNCAPMSASASGNPTSGTAPFTAAFRGIADGGCPPFTYA
                     WDFGDGTTSADQNPSHDYAIQGTFTTTLTITDAKGASAKSTVPVTVSSPLVPTAQKSV
                     VLEGVNFKSNRSTLLPESETILNNVAEILLANPDVNVEVGGHSDADGKDEYNMKLSDA
                     RANAVRNYLITKGVPAERLTARGYGETHPIADNVTPEGKAQNRRVELKRI"
     gene            13848..14216
                     /locus_tag="VEC56_09665"
     CDS             13848..14216
                     /locus_tag="VEC56_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV14460.1"
                     /translation="MTHQRANEPNDLEKFFVERANAGDVEGLVALYEPNAKIVDGDRE
                     VASGAGQIREFFVRFLAGHPQFGPSHQAPALCSGDLALTSSRLSNGDLTAEIARRQPD
                     GSWLWVVDQFVLVDTVYRKP"
     gene            complement(14399..14772)
                     /gene="ssrA"
                     /locus_tag="VEC56_09670"
     tmRNA           complement(14399..14772)
                     /gene="ssrA"
                     /locus_tag="VEC56_09670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14844..16019)
                     /locus_tag="VEC56_09675"
     CDS             complement(14844..16019)
                     /locus_tag="VEC56_09675"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXV14461.1"
                     /translation="MRRDFSLIMRSALGLVVLVVSLARAGEDFDARALRRIQNPPLGL
                     PPVPVPAENPPTPEKIALGRKLFFDRRLSVNNTMSCGMCHVPEQGFTSNELATPVGVE
                     GRTVRRNSPTVINVAYVQQVFHDGRETSIETQPISPLTAHNEMANPSMGFVLARIVSL
                     DDYDGRFERVFGGGPTVDRLGQALSAWQRTLVAANSPFDRWKFGGDENALTAEQKRGF
                     ALFNGKASCSTCHTVDREFALFTDDGFHDTGIGYFREEVAPADASPLPVEMAPGVVIP
                     VERDYLDAISEKREPDLGRFEVTQKPEDKWRFRTPSLRNVAVTAPYMHDGSLRTLEDV
                     VRFYARGGVTHPGLDPLLRPIDLSEHDVAALVAFLQGLTSPDLAELVADARSVAVGD"
     gene            complement(16041..16739)
                     /locus_tag="VEC56_09680"
     CDS             complement(16041..16739)
                     /locus_tag="VEC56_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXV14462.1"
                     /translation="MKRIVVVCAILGLAATAAAAGPAESERPELSIGRSAQYDYDPPV
                     PGSYALPVIDEAGDGEVLTAEGKRARLRDIVRGQVTILSFIYTRCADPRACPMATGAL
                     FQIHGISLEDPEIARGLRLVTFSFDPEHDTPSVMAEYGGMARPGGQGSEWWFLTTRST
                     AELEPILAGYHQRVDRKKNPLDPLGPFYHVVRVYLIDRNGMVRNIYSYGLLDPRMVLT
                     DVRTLLMESSLAGK"
     gene            complement(16752..>17063)
                     /locus_tag="VEC56_09685"
     CDS             complement(16752..>17063)
                     /locus_tag="VEC56_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein"
                     /protein_id="HXV14463.1"
                     /translation="VTTFSDGMVRVYDISDPHHPKQIYEKVIGHQVNMVSQSWDGKRL
                     YFTSSVLSRWDKKDEGSEEYLRAYTWDGKQLTPSFDVDFMAEKLGKPHLMRFGSASLY
                     N"
BASE COUNT         2978 a         5525 c         5422 g         3138 t
ORIGIN      
        1 ggttcgaagt ccgtcaccga ctgcgtgctg gcgcaaatcc acggctggcg cttcccggcc
       61 atcccctacg gcgtcaccag cttccgcgcg ccgttcgtct tcacgcctcc gaactagttc
      121 cgccgaaact accggttggg aaagccggac tgcctgcgct agaatcgcga gggtgaaggc
      181 gagaaccgca gttctcctgc cctgttacaa cgaagccgtg acgatcggac aggtcgttgc
      241 ggactttcgc gccgcgttgc ccgacgccgt cgtctacgtt ttcgacaacg cgtccgacga
      301 cgacaccgcg gcgaaagcgc gcgccgcggg cgcggttatc gtgccgtctc cccgccgcgg
      361 caagggcaac gtggtgaggc acatgttccg caccgtcgac gccgacctct acgtgatggc
      421 ggacggcgac ggcacctacc ccgcgcacgc ggcacccgag ctcattcgcg ccctggagca
      481 aaccgacgcc gacatggtcg tcggcacgcg cttgcagcac agcgcgccca agtcgtttcg
      541 ttccttgcac gggctgggca accgggtcat ctccggcctc atttccagcc tattttcggc
      601 gtcgttgacc gacgtgcttt cgggctatcg cgtgtttcgc cggcacttcg tgcggacact
      661 ctacttgcgg tcgagcggct tcgagatcga gaccgagatc acgctccagg cactcgtcaa
      721 ggagcgggac atcctcgagg tcccggtccc gtacggagcg cgcccggaag gaagccactc
      781 gaagctccgg acctggtccg atggcgcgct cgtactcaag agcatcctcc tcatctttcg
      841 cgactacaag cccctcgcct tcttttcgtg tgtcgccggt gtttgcttcg tgctgggact
      901 tctcgccggc tggcttccgg tcaaggacta catcgaaacc cgcttcgtga gccacgtccc
      961 gctggcgctc ttggccgcgg cactcgaggt gctggccgtg ctgtttcaag gaatcggact
     1021 catcttgaac gccatcaaga gattccacca agagaaccag gaaatgatga gcgcgatcct
     1081 caagcgcttc gaccaggacc atgaaggccc ctgacgacga cgcgcgcggc acgccgtacg
     1141 cggggatcga caacctcgaa gtgctcgcgc acgcggtccg gtacaaccgc tttctggtgg
     1201 acaccatcgt cggcgcggcg caagagcggg gcaaggcact cgatttcggc gcggggacgg
     1261 gctcgctttc gaccatggtg cgggatcgag gcttcgaggt cgcgtgcgtg gagacggacg
     1321 cgcggctgcg cgagcggctc cgctcccagg gattccgggt gtacgacgac gtcgctctgg
     1381 tccccgatcg gtcgtacgcg ttcatctaca ccgtcaacgt gctggaacat atcgaggacg
     1441 atgccggtac tctgcgggtg cttctatcaa agctggagcc gggcggccgc tgcctggtct
     1501 acgtgcccgc actcggtatg ctgtactcca gcatggaccg caagatcggt cactatcggc
     1561 gctaccaccg cgcgggcttc gttcgtgtcg cgacccaggc gggcttcgcg gtcgaacgcg
     1621 cggagtacgc ggacagcctg ggattcttcg ccacactcgt ctacaagctc gtcggaagcc
     1681 gccaaggtga tctgtcgacg gcgtcggtgc gcctctacga ccgcttcgtg tttccgatga
     1741 gtcgcgtctt cgaccgcatg ggatgctcgc gcgtgctcgg gaagaacttg gtagccgtca
     1801 tacggcggcc cgggccgacc ggggaacgcg cgtgacggct gcgaagcgaa tatacctcgc
     1861 ggccttcgcg ctgggcttca tcgcgtgcgg aacggtgtcg gtcctcatgg gccaggacaa
     1921 caactgggac ctgcgcaatt accactgcta caacgcgtat gcgttcctca ccgatcgcct
     1981 cggattcgac tacgcgccgg cgcagaggca gtcgtatctg aatccgttcc tcgacctgcc
     2041 gttctatctg ggcacgatcc acctcgatcc caagctgctg gggttcttgc tgggcgggat
     2101 tcacgggctg agtcttggtc tcttattcgc gatctgtatg atcgtcttcc gagatcattc
     2161 tgcaaaggcc cgcatggttc tgtccgctct gtgtaccggt gccggcctat acggaccggt
     2221 cttcctcggc gagctgggcg cgtcgcaaaa cgacgttctg gtgggcttgt tcgtcctgac
     2281 cgcgttgctc ttgctcgtgc gcacgttggc cgtgcagcaa tcgctcgcgc cgcgcgccgc
     2341 gcgccgcgcg ctggtggtcg cgtcactcgt cttcggcgcg ggcgcggggc tcaaggcgac
     2401 gctgatgccg cacgccgtgg gcctcgcggt cgccgcgtgc gccgtcgaac acacgtggcg
     2461 aaaccgggcg ctggtgctcg cgctcgcgtg cggcccgttc ctggccggct tcctgtcgtc
     2521 gaacgggtac tggatgctcc gcctttggca ggagttcgga aatcccgtct ttccgttcta
     2581 caacgacgtg ttccgctccc cgtgggccga cccgcggtcg tatgccgatc gctcgatgat
     2641 cccgctgtcg atgtgggacg ccgtccgcct tccgttcgcg tttgtcaggg acagcgacta
     2701 cacgtcgctc cagaatggat ttcgcgacac gcggtacgcg gcgctgtacg gcgtgctcgt
     2761 cgtgtacgcc gtgctttggc tgggtcgccg gttcgtccca cgcagagccg gtaggatcga
     2821 gtggcgcgcc atggcgatcg agtcgcgctt cttgctgacc ttcttcgtgg tgtcgttcgt
     2881 cgcgtgggaa gcgatgttct ccattattcg ttacacaccg acgctcgagt tgctcgcgcc
     2941 tctgctcctc gtggtcgtgg gatcccacct gttgggccat ggggtcgcac gaaccgcggt
     3001 cgtcgccacc gcaacgctcg cgctgcttgt cgccaccgtg aagccgattc aacacgagcg
     3061 cctggagtgg ggagagacgt tctgggagat tgaagtgcct cccttgccga accccgagcg
     3121 caccatcgtc ctgatcgcca acgcgcgacc gttggcctac ttgatccccg catttccgcc
     3181 ggaagtccgg tggctgagtc ttgagaacaa cctgaccgag ccctcggatc aaaccagaat
     3241 gcaggccgac atgcgacgcc tgatcacggg ccacgacggc gatgtgtatt tgctctcccg
     3301 cgccgagccg agcccgttgc tcgctcacga cgtggcggtg gcgggatact acggactccg
     3361 gctcgagagc aacacgggac tccccatcgt cagccggcat tccccgccgg ggatgttctt
     3421 gtggcgcctg gagcggtatt aggagagccg gcgccggatg tcattgaccg gttacttcag
     3481 cagcaccatc ttgctcgccc ggtacgcgcc gttttgctcg acgcgcgcga agtagacgcc
     3541 cgacgcagca gaccgatttc cgtccgtgcg gccatcccat tcgacgcggt gatcgccggt
     3601 gcgccggaac tcccgatgca ccagcgtcgc gaccctcgcc ccgcgcgcgt cgaacacggc
     3661 caccgtcacc ggaccgtcgg ccgggacggt gtacgtcaag gtggtgcggg aattgaacgg
     3721 gttcgggtgc gcggccagcg tccacgcggt tggcggtggt acgttgggca cgggagtcgc
     3781 ggcggccgtc acgaggtagc cgccgaccag gacgaattcc accggctgct ctcggcacag
     3841 cacctccgcg atgccggagt cgaagaaccg aaagacggcg tcggcgcggt cgatgtggat
     3901 cgtggacccc gacatctcgc acgtgaccgg gccgtcgaag acggtgacga gcgcgttggt
     3961 tcccacgacg agcgagcgcg caccggcggc caagtaggcg gcgacgacgc cgttctcctc
     4021 gcgcacccac gcgacgagcg cgtcggtctc gatgttctcg tgcgcgaccg ccgcgcccgc
     4081 gtcgtttcga accagccgat cgacgacacc gccaccccaa tcgacggagc acgcgtatcc
     4141 ccacgggtac ggctcgcgcg tcaccgccgg tgacgggctc ccgcccctcc ttggcacgag
     4201 catgagcgcg aagcgggggt cgatcgcggt gtgcgtgacg cgcaagatgg tcgaattggg
     4261 atcggtgttc ccattctcga aggcgctcgt ggtcaccgtc gccgattccg cgctcgggtg
     4321 cagcaggtgg acgtccatgg ccgcgcccgc cccctcgatg gtccatgggc tcgcggccgt
     4381 gttcaccgtg tttccgaagt gcgtgtgcat gcgccactgg tagaggtgcg gcgcgccgtc
     4441 cttgtcgatg tcgtccatga cgactatgta cggcgacgac gcctccccgt gcaccacgag
     4501 cacgcgtcga aacgcgtgct ggaccggatt cgcgccgctg tagccccacg accaatcgac
     4561 gccgggaaac gggacgccgg gcgcgttgaa ctcgctgtac gtcgcgtacg cctgcgtcgc
     4621 gtcccccacc acgtagtcgg cggatccacc cagcagatac tcggcgatgc gtccgtcggt
     4681 cccgatcgac gaaccggcgt tgtgttgccc gagtccatcg accaacacca ggttgtgggc
     4741 ttcgctctcc ttgcccaacc cacccgcgcc gtggtcgatt gcgaacttcg cgccgtaggc
     4801 gtacagcgcg aacgtattct ggtcctcctg cgcgtgcccg ccttggaatt ttccggaatg
     4861 gaacgtgaac acgacgtcgt cgatcgtggt cttggtcgtc tgccagccag tgcggaagtg
     4921 gtacaggccg cgctgcaccc acacccgatg gagcggcaga atatcgtcgg gctggatcgg
     4981 cggcacgttg gtgtgccaca agatcgttcc cgccttgtcg gccatgatgc ccacgtcggt
     5041 gccgtaggtc ccggccgaat gttcccacag ccacgcggcc agcccgctgt tccacttgga
     5101 cgtcgcccag tcgacgtacg tcgtgctgcg cgagaacggc gttgtcaggt aaggcgaatc
     5161 attgagattg tgcgagtacc cgccgccctc gggaagcagc tcatagggca gccactgctc
     5221 gacggcgcgc acggtcgcgt ggtcggcgta cgagtatccg tcgaagcgct cgcgcgcatc
     5281 gaagtagtag accaggttgc gcagcgtcca caacgcgtac aagactccct cgttgtacgc
     5341 cccgcccgga tcgaattggt acgtcaggag attctccgcg atgcgatccg ccatcgccag
     5401 cgccgccgcg agctgctccg cttcggcctc gccctgcaga ctgatcgcgg ccagccccaa
     5461 ggccgccccg aacatcgcgc tgtgattgcc gaggtagggg cgatactcga acgactcgta
     5521 ggcggtggta ccgatcatgg gctcgatgta gcgcaccatc tcgtcgcgca ccaacgcgcg
     5581 ctcggcctcg gtcgcgtccg cgaagaacaa atcgtagccc agcgcgagcg agcgcaggcg
     5641 caggcccgag ttggcctcgt catagtccgg ctcgtacgtg ttgacgaggt acaaggtgag
     5701 caacttgccc tgcgccatgg ccgcggtgtt cggcggcagc tcgaggtgag cgacgaggcc
     5761 caggttgggc atggcatcga gaccgaagta cgggcccagc accgtagtcg gtggcaacac
     5821 cgaatagata ttttgcacgt agttgcggac gtacgtgtac gcctcgtcgt cgcgcccacc
     5881 gtcgtggatc cgtgcgcgaa gcacgggcag ctccgccggg gtgaacagca cgcgggggtg
     5941 gtgcgcaagg tacagcgagt cggtgaggac cggctgcgcc gacgagggac tgctcgtcag
     6001 gagcgccggc accgccagtg ccaggacgta tcgcagagcg cgtcgcatgt gggagcaagc
     6061 tggaaagcgg gacggccggc gcgcagggcg ccgaccgtcg aagtgtacca catggcgggc
     6121 tcggagcgcg cgatcgccgc tagaggccga atcgcatcag cgcgccgaac gtcatgctgt
     6181 ccaggtcgtc gccggtgaga ttctcgagct cctcgtcgct ctggaagcga tagcttgcaa
     6241 acatgtcgag gttgaaaccg cccaacggca ggttcacacc ggcgcgcaag gcatagaacg
     6301 ggtcgttttg ccagtcgccg tccaggtagc cgattccgat tccggcccct ccgtagatca
     6361 tgccgccgac gagcgcgtac gcttgcggct cccacatcgc ctcgccggta ccggcgaagt
     6421 cgaacacgta ttcgaggtcc gcttcaaact tgagaagcgg tccggcaaac tgatacgagg
     6481 ccatgagcga gaacgaattc tcctcgtagc cgtaagtctg gatatcgccc aggtttcgta
     6541 aatagtgaac gccgccgccg atgctgctct ccgcattggc cgcacccaac caacccgaaa
     6601 gcagcacagc cgcgatcgcg atggctttca gaccgtgttt acgcaccgca ggacctccta
     6661 tgaagtggtt cgggtccagc caaggtacga aagcccggtc ccctccgtca atcggaatcg
     6721 ggtgtgcttt gggagatcgt taacgagcgt gcgcctattt gcttcgatac acgtcggtgt
     6781 tgaggtactt gagaccggag tcgcacatga gagtgaccac cttggacgca gggcccagtc
     6841 gctccgccac ttggatcgcc gcgagcacgt tcgcgcccga cgacgtcccc gcgaagagcg
     6901 cttcttcgcg cgcgaggcgt cgcgtcattt ccttggcgtc gtcggtcttg atggggatga
     6961 tgtcgtccac caacgaggga tcgaagagcg gaggcgtgta gccgatgccg actccttcga
     7021 tcttgtgcgg gcccggcgcg ccgccgccca gcacggcgga ctcctccggt tcgacgacga
     7081 tcaacttcac gttcggcttg tgccgcttca acaccgtggc aaccccgcgc gacgatgccg
     7141 cagtacccac gcagtgcacg aacgcgtcga cttccccgcc ggtttggctc cagatctcct
     7201 ctcccagcgc atggtagccc gtgatgctgt cctggttgtt gagctggtcg gtcaggtatg
     7261 tgcgcggttc gcggcccaac tcgcgcgctt tctcgatcat gtcgagaatg agcttcttgg
     7321 tggtgagccc gccctcgctg gggacgaggg tcagctccgc tcccagcgcc cgcatttgat
     7381 cgcgtttctc cgggctgaac gcatcggagg tgacgatgtg gatgcgataa cccttcgcgg
     7441 cgcacaccaa cgcgagcgaa gcacccgtgc tgccgcccgt gtactcgata acggtgtatc
     7501 ccggcttcag ccggccgtcc tcttcggccc gcgagatgac cgcgctcgcc atgcggtcct
     7561 tcatgctccc ggtggggttc tcccactcga gcttgacgta gatctctccg caatggggcg
     7621 gcacgacctt gcgcagccga accatcgatg tgtttccgat cgcctggaga atggtcatcc
     7681 gtcgagcgta ctgcagttcg gtcgatttcg ctacggggtc tcgcgcctcg cttatctcgc
     7741 gaggtgccgc gagacgaacg cgttcaactc ggcgtagtgc tcggcgcact ccggggtgtt
     7801 cgcgagttga gagtagtccg cgtgggccac cccttcgtag acgttgaggt cggcctccac
     7861 accggcccgc cggagcttgc ggtgcgtacg caccacgtcg ctgagcagca ggtcccgcgt
     7921 gcccgagatg aggtaggtcg gcgggaagcc ggtgaaatcg ccgaagatcg gcgagatatg
     7981 cgggtcgtcg taactcttgt ccccgacata gagcgcgatg gcggctttca cgtcgtccca
     8041 gctcacgagc gtgtggtcga tgccgtcgtt gatgaagcgg gcgtcgccgc gcttggcaag
     8101 gtcggcggcg ggcgtgccca cgaacagtgc cgcgggcaac gcgagtccga gctccttcat
     8161 gcgcagcgtg gcgaccaggg tcagcgtcgc gccggccgag gtgccgccaa ggatgatggt
     8221 cgccggcgag tgcgcctcca cgagctcgcg ccacacggcg acgacgtcgt ccatcgacgc
     8281 aggcgctgga tggtcggggg gcatgcgata gtcgatcgtg agcgcgggta tcttgagaga
     8341 ctccgcgatc tgcaacgctt cgggcaaggc ggcgcgaccg ttgccgacga tgaacccgcc
     8401 tccgtgaacg tggacgaaca aacgatgcgc gtgctccggc gcgatccgag gcggtgtcac
     8461 tcggaacact cgcacacccg acattctctc ctccgccacc ttcaggttgt gctccttgat
     8521 gaaggcgtca ccgagttcga tctttgcggc gtcaccgagt gcgatgaacg ccttccacgc
     8581 cgccatcgtg gtgggcgcga gcagcgcgga gggtgccggc atgtcggtga gaatcttgcg
     8641 cagtggctcg ctcgcgcccg cgggcggcgg caacgttcgc ggcccgatcc tccacccctc
     8701 gcccccatcc agggtcgcgg cgacgcgcac ggtcgaagat cgagcgacgg ccgcccgcag
     8761 ggccatggcg agcgcccgcg cattccgaag gttggctttc atgttcgatc tcctgactcg
     8821 agtccgaggc ggacgcccgg cggcggtggg ggaacttcag atgcccagac tgggagggta
     8881 acaggtatag cggctgtgtg cgggtcgatc ttctcgcgac gcttcgcccg gagacctagc
     8941 gctttttcat cgacgcattc tgggccaccg cgaccttcac gagtgctttg aacgcgcgcg
     9001 cgtcgacctt ctccccttcg cggatgtcga tggcccgtcg cgcgttgccc tccaagctgg
     9061 agttgaagag gcgcttggga tccgggaggc tcgccccgcg agcgaacgtg agtttcacga
     9121 ctttcgtgta catctcaccc gtgcacacga acccgtggtg cgaccagacc gggactcccg
     9181 cggggttcga cggctttttc catctccgtt cctcgatcat ctcggggtcg gcctccaaga
     9241 tcagcgcccg catgcgggcc aaggtctcct cgcgccaatc cccgtcgtcg cggatccgct
     9301 tctcgatgcg cttcgaagcg ggcttcgacg tagttcgctt ggtcgctcca ttcttcgctt
     9361 ttgttttcaa aactctcttt gccgccgtgg ccatgcgcgt actcagttct ccaccgcaat
     9421 cacgtagtag ctgatttcga tctcgcaatc gcccgagggg ccagcgaggg tgatggtgtg
     9481 cagacccggg ctgaatcccg ccgatccccg gtagaccttg gtgtcggact ggtcgtccat
     9541 atcgccgtcg ggcaccggat caccgaagtc tacaaactcc aggtcccatt ccgtcaagcg
     9601 gacctcgacc tcgagattct ggatgtcggt gagggtaatc gacgtctggt cggtgggcag
     9661 gttgtagttt gcattctggt cgacgatgac gacgtaatac tccgacggat aatccaccgt
     9721 tccggccacg ggaactcgcg tcccggtcgg cagcacgata ttgatctcat aagaaaactc
     9781 gccgtcgccg ccgtcctcgt tggtagcact gtcgcacgag tacgccgcgt agatgcggaa
     9841 cggttcgacc gtgatctggt attctttcgg aagcggcgca cccacttgca gcaccgcaga
     9901 accatagcga ccgcgccagt gaacgtacaa ctccgacgcc cccgtggaaa cgttcacggt
     9961 cgcgatgccg aagtccgaca ccgagatcgc ttccgggttg ctggcctccc acaggacacg
    10021 atcgctgacg tccatgtccg tggcgtccgg caaccgcacg gtggcaacga gctggcgcgt
    10081 ctcgccccgc acactgaact ggatgatatt gttgaggtcg ccaccctcga tcttcacctc
    10141 gccgaccccg ggggcccgcg tgtacacgaa gaacggatcc gtgaacgtcg tgccgcggta
    10201 ccgaatgcgc aggtcggatc ggccggcctc gatggtcgtt accatgccct ggttgaccgt
    10261 ggccttggac gggtccagcg tctcccacgt cgcctcgttg gtcacgtcgt gcgtggtgcc
    10321 gtcttccagc agtacgtagg cctgcatctg cccggaattg cccgcgacca gccccaggct
    10381 cccttcgatc cagactctcg taatcgcggg cccctcttcg ccatccggcg cggtggacga
    10441 atccgagcta cagccggtga acaccggcaa aaggataacc ataagcagaa taacgacgtt
    10501 cgggagtttg cggaccatga tcatgacctc ggtttcgggt attttgcctg ctatcagtgg
    10561 acgcaggaaa tgacgccgaa agcgatcacg gaaaagaggt ctcggaacgc cgacggcatg
    10621 gaccatctcg cggcaatgct gttaaaggtt aactaattgt agcacacatg acagaacggc
    10681 ggtcagaaag gaaacttatc gcactttcgc ttgctcgccc cgtaggacga agcaggtgga
    10741 gctggccttc gcgacgtgct ttccgtctcc gtcggttact tcgcattcaa catagccgac
    10801 gcttttccct cggttgacca cccgagcttc cgcgcgcaga accgtctgcc acacgggccg
    10861 gaagaagctg atgttcagct cgatcgtggt gaacgattcg tcgggcgcca aggtcgatac
    10921 gaacgccata cccatggccg cgtccgccaa gtcgcacagc acgccgccat gcagcgtccc
    10981 catcgggttg gcatgctgcg gccccgactg catcgagccg acggcgcgcc cgccggtcat
    11041 ttcctcgacg cggaagccga tgagctccgc gatgggtact ttggaggaga gtacttttgc
    11101 gatcagaggg tggacaggtt ctttcatgct tgcagtggtg aaggtaggag gatagtgcct
    11161 ggatgcgtcg tcgtcaaggg acccgaaaac gtcgcactcg aagagccgcc gcggcgaggc
    11221 acacgtcggc caccagcgcc accagcggcg gcgtccacct gttcgtcaag aacagtgctc
    11281 tgaacagaag caagtacgcg gctccgataa caagccccga gccgagtgcg agcaacgtgg
    11341 cccccacggc gctcgacttt cggtggatcc cgaagaagcg ccccgcaagc gacgagctca
    11401 agaaggtcaa gatgagagga gacgcattga ggctcgcgaa gggcaactcc gggctatcca
    11461 tcatggaggg tgcagtcaag agatagatgc ccacggcgag aaacccccac gccaggccgt
    11521 tgttcagccg cacgaccgcc gtcccgataa tcacgccgac catcaccgag atcaccacgg
    11581 tcccggcgac cgttgccaat gcgccgtatg ctaccggcgc catcgcgttg ccccgtcgac
    11641 ttcgcttgca catgcgccac ccgtgacgag cacgggcatg cacgcgacaa cgggtccgtt
    11701 atgggaatga ccgctcacca cgttcctccg cgggccatgg ggagggtatg ccccctggga
    11761 acgtgcggtc aaccttcgcg gggccctgcc cgaataaggt cttgcacccg ccgccttctt
    11821 gggtccaccc tccggcgggg agtgcgcact cgaacttgcc gatctgcccg aaccattcga
    11881 attgcgggtg cgcacgacaa cgggcccatg gacgcaccat cttcgcgtaa aacacagcgg
    11941 gcgtccgttt ctccgctcga cgattgctct caagaggagc gctacgtcat caggccactc
    12001 cttgaaagca cgaccaggag gtaccatatg ttgaaatccg tcaagtatgg cgttggggtc
    12061 gccgcggtga tgtggccggc cttggctgcg agtccgttgc acgcggcgga tgtggatggg
    12121 caatgggcca ttggcttgcg tcccggattg tacaagctcg tcctcaccga ccactcggac
    12181 gcgtggacgc cgggtggttt gatcaacgtc gacgtcaagt acggaatgtc gtcgaagttc
    12241 tcgctcggcg tcgagggaag ctggatgaag acctacgccg ccgacttgag cgccaaaccg
    12301 ggagacggcg ccgggtcttc cttcgacaag attgacggcg gccctcagca acgcgggacc
    12361 gtcattggac tgatcggcga gtaccagttc agcgaagacg gcacgtgggc accctacttc
    12421 gacgtcgggg ccggaatgta tttttggaag tggaccgacg ccgacggcaa caccctcctc
    12481 tccgacgacg ccgcgctcga cgatccgctc gggggtctct acgcagttcc agatgcagac
    12541 caagcgggca atccgtatga attgaaggac caagagctgt acctgatggc cggccttggc
    12601 atcgactact acgcctccga cctcatctcg atcgggctgg gcgtcaagtt ccggtatctc
    12661 acgcgcgtgt tcaccgactt caccgacgac aaagacatcg ttggatcgga cccgggccaa
    12721 ctggatctgc cgaaggggat cgtcgaggga ctcctcggcc tgacgttcca cttcggaggg
    12781 aagtgcccgg agatgtcggg taccggttcg cccagcgcga ccaccggcat attccccatg
    12841 gacgtgcagt ttgagtcttc cgcgacgggc ggatgtcccg agtacaccta cgcgtggaac
    12901 ttcggcgacg gcaccacgag cacggaggcg aatcccagtc acacgtacgc ccagatgggc
    12961 acgtattccc cgtcgctgac gatcacggat gccaaaggca attccgccct ggccgcggta
    13021 ccttcgatca cggtgaactg cgcgccgatg tcggctagtg cctcgggcaa tcccacgagc
    13081 ggcacggcgc cgttcacggc cgcgttcagg ggcatcgccg acggcggttg tccgccgttc
    13141 acgtatgcct gggacttcgg agacggtacc accagcgccg atcagaatcc gagccacgac
    13201 tacgcgattc agggaacctt caccacgacg ctgaccatca ccgacgccaa aggcgcgagt
    13261 gccaagtcca cggtcccggt aacggtttca tctccgctgg ttccaaccgc gcagaagtcc
    13321 gtggtgctcg agggcgtcaa cttcaagtcg aatcgatcca cgctgctgcc ggagtcggag
    13381 acgatcctga acaacgtcgc cgagattctg ctggcgaacc cggacgtgaa cgtggaggtc
    13441 ggcggtcata gcgacgccga cggcaaggac gaatacaaca tgaagctatc ggacgcgcgc
    13501 gccaacgccg tgcgcaacta cctcatcacg aagggcgtgc ccgccgagcg gctgaccgcg
    13561 aggggttacg gcgaaacgca tccgatcgcc gacaacgtca cgcccgaggg caaggcccag
    13621 aaccgccgcg tcgaactaaa gcggatctag gcaagatcgt tgggatcgga cagcgttcat
    13681 tgacacggga gcccggtcac cgaggtcctc ggtggccggg cgttttctga gtccgagaga
    13741 aatcaggatg aaccaccgtg atgtgatccc gatgcgcccc tccacgttct caatcgtcgt
    13801 tttcgccaga ctcggaaacg tgatcatcgc tgggccggag gtccaaattg acgcaccaga
    13861 gagcgaacga accaaatgac ctggagaagt ttttcgtaga gcgtgccaac gcgggcgacg
    13921 tcgagggtct ggtcgcgctc tacgagccga atgccaagat agtggacggt gaccgagagg
    13981 tggcatcggg ggccggtcag attcgcgaat tcttcgtcag gtttctcgcc ggtcacccgc
    14041 agttcggtcc cagccatcaa gccccggcgc tgtgcagtgg ggatctcgcg ctcacatcgt
    14101 cccggctaag caatggagat ctcaccgccg agattgccag gcgccagcca gacggtagtt
    14161 ggctctgggt ggtagatcag ttcgttctcg tagacacggt gtatcgcaag ccctgatcgg
    14221 cgagctcttg gatggtcggt gaaggcgtct gttgcggcga tcgagatccg gggacagcgc
    14281 ctcccagacg cagtgctggc cttctcggat gtcgaggagc ccacgaagag gtgagagagc
    14341 ctgcgctaca ttcaataagg agtgttaatt cgagacgaat gtcctgacta catgtaagtg
    14401 gtggaggcgg cgggaatcga acccgcgtcc gaagaagcag ccgcaacgat tactacgtgt
    14461 gtagtccgtg gtgatttctc gtctccgtcc ccaccgcgga cgaaagtgta acggagacca
    14521 gcccggtaga gtctcgcgcc tcggccccag acatagcttc ggcgcacagc ccccagagta
    14581 tgacgcccac tcaccggccc tggagaccca accggtgggg acgggtgact aaactagctt
    14641 atgctagtta ggcagccagt gcgacatgat cgtcagcact tgattggttg ccacgaggat
    14701 ttacgagcat tgtgacgttg ctcgacacgc aaccgttacg tccatcttcc ccgtcgagac
    14761 ctattcgccc ccatcgtgga gtggacttgg tagcgcatca aagatagcag gaagcggacc
    14821 aggcggcaag gcggcggcgg gcgctagtcc ccgaccgcga cgctgcgcgc gtcggcgacg
    14881 agttcggcca ggtccggact ggtcaggcct tgcaggaacg cgaccagcgc ggcaacgtcg
    14941 tgctcggaga gatcgatggg ccggagcaac ggatcgagtc ccgggtgggt cacgccaccg
    15001 cgcgcgtaga agcgcacgac gtcctccagg gttcgcagcg acccgtcgtg catgtagggt
    15061 gcggtgaccg cgacgttgcg caacgacggc gtgcggaaac gccacttgtc ttctggtttc
    15121 tgtgtcacct cgaagcgccc caagtcgggt tcgcgtttct cgctgatggc gtccaggtag
    15181 tcccgttcga ccggtatgac gacgccgggc gccatttcga ccggcaaggg cgacgcatcc
    15241 gcgggtgcca cttcctcgcg gaagtatccg atgccggtat cgtgaaaccc gtcgtcggtg
    15301 aagagcgcga actctcgatc cacggtgtgg caggtagagc agctcgcctt tccgttgaag
    15361 agggcgaagc ctcgtttctg ctccgcggtg agcgcgttct cgtcgccccc gaatttccag
    15421 cgatcgaagg gagagttcgc ggccacgagg gtccgctgcc aggccgagag cgcctgcccc
    15481 aggcggtcca cggtggggcc gccgccgaat acgcgctcga accggccgtc gtagtcgtcc
    15541 agcgacacga tgcgcgccag cacgaaaccc atcgacggat tcgccatttc gttgtgcgcc
    15601 gtcaacggac tgatgggctg ggtttcgatc gaggtctcgc gaccgtcgtg gaaaacctgc
    15661 tgcacgtacg ccacgttgat aaccgtcggg ctgttgcgac gcacggtccg cccctccacg
    15721 cccacgggtg tcgcgagttc gttgctcgtg aagccctgct cgggaacgtg acacattccg
    15781 cacgacatcg tgttgttgac ggacaggcgg cggtcgaaga agagcttgcg gcccagcgcg
    15841 atcttctccg gcgtgggcgg attctccgcg ggcacgggaa cgggaggaag tcccagcggc
    15901 ggattctgga tgcgtcgcag tgcgcgcgcg tcgaagtcct cgcccgcgcg ggctagggag
    15961 acgaccagga ccacgagtcc cagcgcgctc cgcatgatga gcgagaaatc acgccgcatc
    16021 gcactccgac catgtcgcgg ctacttgccg gcgagcgacg actccatgag cagagttcgc
    16081 acgtcggtga ggaccatccg ggggtcgaga aggccgtacg agtagatgtt ccggaccatc
    16141 ccgttgcgat cgatcaggta gaccctgacc acgtggtaga acgggcccaa cggatcgagc
    16201 ggattcttct tgcgatcgac gcgctggtgg tacccggcga ggatcggctc gagctcggcg
    16261 gtgcttcgcg tcgtcaagaa ccaccactcg gatccctggc cgcccggccg cgccatcccg
    16321 ccgtattccg ccatgaccga cggggtgtcg tgctcggggt cgaagctgaa cgtcaccaaa
    16381 cgcagaccgc gcgcgatctc cgggtcctcg aggctgatgc cgtggatctg aaacagcgcg
    16441 ccggtcgcca tggggcacgc gcgcggatcg gcgcagcggg tatagatgaa gctgagaatc
    16501 gtcacctgac cgcgcacgat atcgcgcaag cgcgctcgct tgccctccgc ggtcagcacc
    16561 tcgccgtcgc ccgcttcgtc gatgacgggc agcgcgtagg agccgggaac gggcggatcg
    16621 tagtcgtatt gcgcgctgcg tccgatcgac aactcgggcc gctcggactc ggctgggccg
    16681 gccgccgcgg ccgtcgccgc caggccaagt atcgcgcata caactacgat ccgcttcatc
    16741 ccgctacact cctagttata gagactcgcc gaaccgaaac gcatcaggtg cggcttgccg
    16801 agcttctcgg ccatgaagtc cacgtcgaac gacggcgtca gctgcttgcc gtcccaggta
    16861 taggcgcgca agtactcctc gctgccttcg tccttcttgt cccaccgcga gagcacggag
    16921 ctcgtgaagt agaggcgctt gccgtcccag ctctgcgaca ccatgttcac ctgatggccg
    16981 atcaccttct cgtagatctg cttcggatga tgcggatcgg agatgtcgta cacgcgcacc
    17041 atgccgtcgg agaacgtggt gac
//
LOCUS       DASPGK010000346        12420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1139118, whole genome shotgun sequence.
ACCESSION   DASPGK010000346 DASPGK010000000
VERSION     DASPGK010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139118"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2490)
                     /gene="uvrA"
                     /locus_tag="VEC56_09690"
     CDS             complement(<1..2490)
                     /gene="uvrA"
                     /locus_tag="VEC56_09690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXV14464.1"
                     /translation="MKQKHLVVRGAREHNLKNISVTIPRNQLVVVTGLSGSGKSSLAF
                     DTIYAEGHRRYVESLSAYARQFLGQMEKPDVDRIEGLSPAISIEQKTTGRNPRSTVGT
                     MTEIYDYLRLLFARTGTPHCPSCGRVIAQQSIQQMQERILGYPAGTRVAILAPLIRGR
                     KGEHKKVLETIESQGYVRARVDGVMYEIDQVPALHKQKKHDIDAVIDRLTVDAKNARR
                     IADSLEAATKLSGGTVTVVAGGEEHVFSQQFACANCGTSFGELSPRLFSFNSPYGACE
                     ECGGLGSRMDFNEALVVPDGDLSLDAGALAPMGKLKGDWGRSQLDALGEHVGFQLNTP
                     WSKLSKRARDAILYGIEDEISVKMDFAKVQGEYRTTYEGLIPWLRRRYKNTSSESIRK
                     WVETFMTVEACAGCGGARLKPEALAVRVGPHTIADVVRMSVSRGLFEMNALTLEGNAA
                     IIGAPILHEIVSRLRFLADVGLGYLSLDRSSATLAGGEAQRLRLATQIGSKLMGVLYI
                     LDEPSIGLHHRDNARLIKTLKDLRDIGNTVLVIEHDRDTILAADYVIDLGPGAGIHGG
                     EVVAVGTPDEIRKVPESLTGKYLRDDRPPITRGARASLIKEAIVVRGARENNLKNIDV
                     EFPLGVFTCVTGVSGSGKSTLVNDILFRALHRIHYGGNVLPGAHDALAGVSHIDKVIE
                     IDQSPIGRTPRSNPATYTGVFTPIRDLFAQLPDARARGYKVGRFSFNVEGGRCEKCQG
                     DGVIKIEMHFLPDVYVTCEACKGRRYNHETLQITYKGRSIADVLDMSVEEAAEVFASF
                     PLIKRVCETLVSVGLGYIKLGQQATTLSGGEA"
     gene            complement(2553..3155)
                     /locus_tag="VEC56_09695"
     CDS             complement(2553..3155)
                     /locus_tag="VEC56_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14465.1"
                     /translation="MRTHAIVLCLCLAAVVPGSSLADVVVTEMFHVDPGWEAGGYWEL
                     TVSNLTGSGIYMIAVGNTGADLTVVRYPDLVGIWQPMRVSQDEWDTNDWPRQLAEFTT
                     PDTSLLPFATEFPGHTQALVYYVIGSNPPLADGAVLTGLYFNYPIGMRSASGRAVNSS
                     AGSPFLVFGSSGEVVARGQTVGTPLPARASTWGGIKALYR"
     gene            3265..3834
                     /locus_tag="VEC56_09700"
     CDS             3265..3834
                     /locus_tag="VEC56_09700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV14466.1"
                     /translation="MPPTLRLATPADATGCLDIYRPFVESSHTTFETEVPSPDEFARR
                     IEVTLTQRPWVVAEEDGRILGYAYASPIKDRLAYQWSAEVAIYVAADARRRGLGRALY
                     EALFRCLAGLGYVNAIGIIALPNDASIALHESLGFQKIAHLKEIGFKRGAWHDSGWWQ
                     KRLAPLPARPTPPRPLAECRDEAVRWIRD"
     gene            complement(3905..4573)
                     /locus_tag="VEC56_09705"
     CDS             complement(3905..4573)
                     /locus_tag="VEC56_09705"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008049604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV14467.1"
                     /translation="MSLQIGDIAPDFEAQTTEGRVQFHEWLGDSWGVLFSHPKDFTPV
                     CTTELGYMAKIKPEFDKRNVKIMGISVDSVEDHQRWKGDIKEATGAAVTYPMIGDSEM
                     SVAKLYGMIHPNASGSASTRTAADNATVRTVFVVGPDKKIKLMLTYPMSTGRNFDEVL
                     RVIDSMQLTARHSVATPVNWKRGDDVIILASVNDEDAKKKFPGGWKAPKPYLRIVPQP
                     EDRI"
     gene            complement(4738..5754)
                     /locus_tag="VEC56_09710"
     CDS             complement(4738..5754)
                     /locus_tag="VEC56_09710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV14468.1"
                     /translation="MRTILLRLALVPALLVAASCQRAPELVEQATVDPLPSWNETASK
                     RAIMDFVRRVTTDGGPDYVAPAERVAVFDNDGTLWAEQPIYFQLAFAFDQVTAMAPEH
                     PEWAEKEPFASLLKGDMAGVMAGGERSLMEIIAVTHAGMTTDEFERSVRSWIATARHP
                     TTQRLYTDMVYQPMLELLDYLRAKGFQTFIVSGGGVEFMRAWAEEVYGIPPEQVVGSS
                     GKLKWEMRGTGPVLVKTAEVEFVDDKEGKPAGIQRFIGRRPTFAFGNSDGDHPMLQWT
                     AAGDGTRFVGIVHHTDSEREWAYDRESHIGKLANALDEAMAKGWTVVDMKNDWKTIFP
                     PAAP"
     gene            complement(5757..9353)
                     /locus_tag="VEC56_09715"
     CDS             complement(5757..9353)
                     /locus_tag="VEC56_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV14469.1"
                     /translation="MNIRRCYASFSVEFWHSFRRPLFAMLALIVILTAFGLASGHMQI
                     SSGETSVGGKKAWITSEFAQTQTMTYLVLLYYAFFIAVAAGLTLLRDREVKVDVLLHS
                     TPLTPAEYVWGRFLAVIASFVVLMGLQIAATAFFNHAVPNADAHEMRGPFHFANYAIP
                     VVTIGLPFLVFFTGMSMVIGERTRNAVLVFVLPVATLLVSGFFLWTWSPSWLDLRVNQ
                     VLQVIEPSGYRWLNETHLKVDRGVDYYNTQRVPYDGLFGLNRLWLLLVGVGAVVLTVR
                     SVEQSIRGAVVSKRAARKRRKVAEASEWEEARAGALLALGMKSGTPSFVATVRRVAGA
                     ELRELRRQAGLYIFVPLILLQSLGNVLFAVGAFDTPLLLTPGTTAATLANQASTFVCL
                     LLMFYTVEALERERTTGFAPILYATPVRTAALLIGKALANGVVVLIVLLASLAACAIA
                     LAIQGTVPFALTPYLLLWGLLLIPTFVGWTAFVTAAYAAVGNRYGAYALSLALLILTG
                     YRALTDKINWAGNWMLWGAVRWSDLGFFETDRVAIVLNRLFVLGCAVFFTVVAVKLFA
                     RRGADAVRTMHALAPRRVLRTGARLLPYAAVPLVAAIALAYHVSQGIEGGAAKKAEKN
                     YWAKNLKTWLDAPVPDLARVDVAVALDPERHSLSSKGSVTLVNPHDTTLVKIPLTGAR
                     HWKNLAWTMNGEAYTPDDSQLLFLFTPPQPLAKGDSVVIGWSFDGRYPGGITRKGGST
                     REFILPSGVVLTGFSPSFMPVIGFQEQVGETKENRTEPRQYPRDYWKGITRASYGATA
                     WFPARITVTGPEEYTLNSVGVCTSNTVENGMRTQVWETDYPVKILNIVCGRWEAKQGE
                     SSTVYYHPAHAYNVDEMSKVLDASRTWYSEWFTPYPWRELKLSEFPGLANYAQGFGTN
                     ITFSENIGFLTKNDAKTDASFLVTAHEAAHQWWGNILTPANGPGGDILAEGTAHFSTL
                     LLFEQVKGPRARMEFAKGIETRYNNRRRADDERPMYDIDGKRDSDNTIIYDRGGWVFW
                     MLYDFMGVERARTAYRNFFQTWSTSRDHPALQDFVAAMRPFAEDATAYDAFTKQWFEG
                     KVVPEYRVASASKVANGDGFDVTVTVENVGTGVMPVEVAATAGERWNEPDSTDGAYTA
                     NPDFQDARGVVTLGAGESKTLTIHCDFDPEKVVVDPDVRVLQLKRKHAFADL"
     gene            complement(9350..10267)
                     /locus_tag="VEC56_09720"
     CDS             complement(9350..10267)
                     /locus_tag="VEC56_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14470.1"
                     /translation="MLSARALTKIYPGPVTALNGIDLDVSPGMFGLLGPNGAGKSTFM
                     KILAGLLEPTSGSVTLDGVDVLTDPKWLWPRLGYLPQEFGFYPHLSGEAMLAHLLALK
                     GVEAPQGTKKLVAALLDRVNLTFAAKRAVKGYSGGMRQRLGIAQAIAGDPRLIIVDEP
                     TAGLDPEERLRFYQLLSELAADRIVLLSTHIVEDVAVLCPRFAVIREGRLIALTTPSE
                     ARAAIEGHIYEGTVDVADLPRLRSERTVTQALLVEGRNRARIHEPKGSPPHGFVPVPV
                     TLEDAYLVLMRLGELRGIDAGGVHAGGAR"
     gene            complement(10399..11799)
                     /locus_tag="VEC56_09725"
     CDS             complement(10399..11799)
                     /locus_tag="VEC56_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HXV14471.1"
                     /translation="MKVARVLVTIFAGATLASCSSKEAITPAEVRAIAKEAYIYGFPM
                     VDSYRIMHAYFVDGTNPEFKEDWNRIRSIARVFTPDDKAVQTPNSDTPYSMLGADLRA
                     EPLVLTLPAVEKGRYYSAQFIDLYTFNFAYAGSRATGSEGGNFLLAGPGWQGETPPGI
                     TQVIRCETELAMVAYRTQLFGPKDIKNVEEVQIGYRVQPLSRFLGTPAPEPAPPIAFV
                     KPLSAQEERTSLEFFGILNFVLQFCPTHPTEGELMARFAKIGVGAGLSFDAAARKPEI
                     RKALEDGRADAWAASDSVNQLLIAGQISSGDLFGTRESLADNYLYRMTAAVYGIYGNS
                     KEEALYFGYGVDSGGQALDGTVGNYTLRFSPNQLPPVSAFWSLTMYDAKTKLLVPNSI
                     ERYLINSPMLPKLRKDKDGGLTLYMQYASPGPGKQTNWLPAPAGPFMMVLRLYGPKPA
                     AYDGTWKRPEVQRVER"
     gene            complement(11896..>12420)
                     /locus_tag="VEC56_09730"
     CDS             complement(11896..>12420)
                     /locus_tag="VEC56_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010386800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV14472.1"
                     /translation="LSAILIYLVLAAQFESFRDPFVILAGSVPLALSGSLIFSFLGFT
                     TLNIYSQVGLITLVGLVAKNGILIVEFANHLQMEGRDKTTAVIEAAGTRLRPILMTTA
                     ATVMGHLPLVFAHGPGAGARNSIGIMLVSGMIIGSVFTLFFVPAIYVLVAKTRRATVA
                     RAVDENGDELATAA"
BASE COUNT         2117 a         4077 c         4065 g         2161 t
ORIGIN      
        1 ggcctcaccg cccgacaggg tggtcgcctg ctggcccagc ttgatgtagc ccagccccac
       61 cgagaccagc gtctcgcaca cgcgtttgat caacggaaaa ctcgcgaaca cttcggccgc
      121 ctcttcgacg ctcatgtcga gcacatcggc gatgctgcgc cccttgtacg tgatttgcag
      181 ggtctcgtgg ttgtagcggc gccccttgca cgcttcgcac gtgacgtaca cgtcgggcag
      241 gaagtgcatc tcgatcttga tgacaccgtc gccctggcac ttctcgcagc ggccgccctc
      301 gacgttgaag ctgaagcgcc ccaccttgta gccgcgcgcg cgcgcgtcgg gcagctgcgc
      361 gaagagatcg cggatgggcg tgaacacgcc ggtgtaggtg gccggattgg agcgcggcgt
      421 gcgcccgatg ggcgactggt cgatctcgat caccttgtcg atatgagaga cgcccgccag
      481 ggcgtcatgg gcccccggaa gcacgttccc gccataatga atgcggtgca acgcacggaa
      541 aaggatgtcg ttgacgagcg tactcttccc cgacccgctc actcccgtta cgcacgtgaa
      601 cacgcccaac ggaaactcga cgtcgatgtt cttcaagttg ttctcgcgcg ccccgcgtac
      661 gacgatggct tccttgatga ggctcgcgcg cgccccgcgc gtaatgggcg ggcggtcgtc
      721 gcgcaggtac ttgccggtca ggctctccgg aaccttacgg atttcgtccg gggttcccac
      781 cgcgaccacc tcgccgccgt ggatgccggc tcccggaccc aggtcgatga cgtagtccgc
      841 cgccaggatg gtgtcgcggt cgtgctcgat gacgaggacg gtgttgccga tgtcgcgcag
      901 gtccttgagg gtcttgatca agcgcgcgtt gtcgcggtga tgcagcccga tcgacggttc
      961 gtctaggata taaaggacac ccatcaactt cgacccaatc tgggtcgcca gtcgcaggcg
     1021 ctgcgcctcg ccgcccgcga gggtggcgga gcttcggtcc agcgacaggt agcccaagcc
     1081 aacatcggcc aaaaaccgca gtcgcgacac gatctcgtgc aggatgggcg cgccgatgat
     1141 ggcggcgttc ccttccagcg tgagcgcgtt catctcgaag agcccgcgcg agacgctcat
     1201 gcgcaccacg tcggcgatcg tgtggggccc cacgcgcacc gcgagcgctt cgggtttcaa
     1261 acgcgcgccc ccgcagcccg cgcacgcctc caccgtcatg aacgtctcga cccacttgcg
     1321 aatgctctcc gacgaggtgt tcttgtagcg acggcgcaac cacgggatca agccttcgta
     1381 ggtcgtgcgg tactcgccct gcactttggc gaagtccatc ttgaccgaga tctcgtcctc
     1441 gatgccgtag aggatggcgt cgcgcgcgcg cttggacaac ttcgaccacg gcgtgttgag
     1501 ttggaagccc acgtgctcgc cgagcgcgtc gagttgcgag cgcccccagt cacccttgag
     1561 cttccccatc ggtgcgagcg cgccggcatc cagcgacagg tccccgtcgg ggacgaccag
     1621 cgcttcgttg aagtccatgc gcgagcccag gccgccgcac tcctcgcacg cgccgtaggg
     1681 cgaattgaac gagaacaagc gcggcgacag ttcgccgaag ctcgtgccgc aattcgcgca
     1741 cgcgaactgc tgcgagaaca cgtgctcttc gccgcccgcg accaccgtca cggtgccgcc
     1801 cgagagcttg gtcgccgctt ccagggaatc cgcgatgcgg cgcgcattct tcgcatcgac
     1861 ggtcaggcga tcgatcacgg cgtcgatgtc gtgcttcttt tgcttgtgga gcgcgggtac
     1921 ctgatcgatc tcatacatca cgccgtcgac gcgcgcgcgc acgtagccct gcgactcgat
     1981 ggtctcgagc accttcttgt gttcgccctt gcgaccgcga atgagcggcg ccaggatcgc
     2041 gacgcgcgtg ccggccggat atcccaggat gcgctcctgc atctgttgta tcgactgctg
     2101 cgcgatgacg cggccgcagc tgggacaatg cggtgtcccg gtgcgcgcga agagaagacg
     2161 gagatagtcg tagatctccg tcatggtgcc gacggtggag cgcgggttgc gcccggtggt
     2221 cttctgctcg atggagatgg cgggcgagag cccttcgatg cgatcgacgt cgggcttctc
     2281 catctggccc aggaactggc gggcgtaggc ggagagcgac tcgacgtaac ggcgatggcc
     2341 ctcggcatag atggtgtcga aggcgagcga ggacttcccg gagccgctca agccggtcac
     2401 gaccaccagc tggttgcgcg ggatggtgac gctaatgttc ttgagattgt gttcgcgggc
     2461 gccccggacg acgaggtgtt tctgtttcat cgcgggctgg cgctaaggta acaaaaccgg
     2521 ggggcgcggg caaccgcccc ccggtcacca aatcaacggt acaacgcctt gatgccgccc
     2581 caggtactcg cccgcgccgg aagcggcgtc cccaccgttt gcccgcgtgc cacgacctcg
     2641 ccggagcttc cgaagacgag gaagggcgac cccgcagacg agttcaccgc gcgtccggag
     2701 gccgaccgca tcccgatcgg gtagttgaaa tacaaccccg tcaataccgc gccgtccgcg
     2761 agcggggggt tggagccaat cacatagtag accagcgcct gggtgtgccc ggggaactcc
     2821 gtggcaaacg gcagcagcga cgtgtccggc gtcgtgaact cggccagctg gcggggccag
     2881 tcgttcgtgt cccactcgtc ctgcgagacg cgcatcggct gccagatacc caccaagtcg
     2941 ggatagcgga cgacggtcaa gtcggcaccg gtgttaccga cggcgatcat gtagattccg
     3001 gaaccggtca ggttgctgac agtcaactcc cagtagccgc cggcttccca cccggggtcg
     3061 acgtggaaca tctccgtgac gacgacgtcg gcgagcgacg accccgggac gacggcggca
     3121 agacacaggc aaagcacgat cgcatgggtt cgcatacgat cctcctatgg ttggattgtg
     3181 gactctgata agtgtacaca aaaccggccc cgatgttggc tgccgccctg gaccttggat
     3241 ggccggccgc ggtatacatt cgccatgccg cccaccctcc gactcgccac ccccgccgac
     3301 gccaccggct gcctcgatat ctaccggccc ttcgtcgagt cgtcccacac caccttcgag
     3361 accgaagttc cctcgcccga cgaattcgcg cggcgcatcg aggtgaccct cacgcagcgg
     3421 ccctgggtcg tcgccgagga agacggccgc atcctcggct acgcgtacgc gtcgccgatc
     3481 aaggaccgcc tcgcctatca atggagcgcc gaagtcgcga tctacgtcgc ggccgacgcg
     3541 cggcgccgcg gccttggacg cgctctgtac gaagcgttgt ttcgctgcct cgcgggactc
     3601 gggtacgtca acgcgatcgg gatcatcgcg ctccccaacg acgcgagcat cgcgctgcac
     3661 gaatcactcg ggttccagaa gatcgcgcac ctgaaggaaa tcggattcaa gcggggcgcg
     3721 tggcacgact cgggctggtg gcagaagcga ttggcgccgc tgcccgcgcg cccgactcca
     3781 ccgcgtccgc tcgcggaatg ccgcgacgaa gcggtgcgct ggattcggga ctagatgcac
     3841 gaagcccccg ccggttgaag gtgggggctt cgctcgtcgg tgcgcgaggg gcgtcgcgcg
     3901 gcgactagat tcgatcttcc ggctgcggga cgatgcgcag atacggcttc ggcgccttcc
     3961 atccgcccgg gaacttcttc ttcgcgtcct cgtcgttgac cgaggccagg atgatgacgt
     4021 cgtcgccgcg cttccagttg accggtgtcg ccaccgaatg gcgcgcggtc agctgcatcg
     4081 agtcgatgac gcgcagcacc tcgtcgaagt tccgccccgt gctcatggga taggtgagca
     4141 tgagcttgat cttcttgtcg ggtccgacca cgaacacggt gcgcacggtg gcgttgtcgg
     4201 ccgcggtgcg cgtcgacgcc gacccgctcg cgttggggtg gatcatcccg tagagcttgg
     4261 ccaccgacat ctcgctgtcg ccgatcatcg ggtaggtgac cgctgcgccg gtggcttctt
     4321 tgatgtcgcc cttccagcgc tggtggtctt cgaccgaatc gaccgagatg cccatgatct
     4381 tcacgttgcg cttgtcgaac tcgggcttga tcttggccat gtagccgagc tcggtggtgc
     4441 acacgggtgt gaagtccttg gggtgcgaga acaaaacgcc ccacgagtcg ccgagccact
     4501 cgtggaactg gacgcggcct tcggtggttt gggcttcgaa gtcgggggcg atgtcgccga
     4561 tttgtagaga catgggggtt cctcctttta tatgcgcggg ccgaggcatt gcggctcccg
     4621 cgtcgtgata gggtaccgtc gttagacccg gatgggtgaa ttacgtcaca agaaatcttc
     4681 gggttcccga ttagtctcaa acgaaacatc gttgcgtgtt gagtgcaacg gcttcgctca
     4741 cggggcggcg ggcgggaata tcgtcttcca gtcgttcttc atgtcgacca ccgtccagcc
     4801 tttcgccatc gcctcatcca acgcgttggc gagctttccg atatggctct cgcggtcgta
     4861 cgcccactcg cgctcggagt cggtgtggtg cacgatgccc acgaatcggg taccgtcgcc
     4921 ggccgcggtc cattgcagca tcgggtggtc gccgtcggag ttgccgaacg cgaaggtggg
     4981 ccgccgcccg atgaaccgct ggattccggc gggcttccct tccttgtcat cgacgaactc
     5041 gacttcggcg gtcttgacga gcacggggcc ggtgccgcgc atttcccatt tcagcttgcc
     5101 gctgctcccc accacctgtt cgggcgggat gccgtagacc tcctcggccc acgcgcgcat
     5161 gaactccacg ccgccccccg aaacgatgaa ggtctggaac cccttggcgc gcaggtagtc
     5221 gagcaattcg agcatcggct ggtagaccat atcggtatac aggcgctgcg tggtggggtg
     5281 acgcgcggtc gcgatccagc tcctgacgct ccgctcgaac tcgtcggtcg tcatgcccgc
     5341 gtgcgtgacg gcgatgatct ccatcagcga cctttcgccc ccggccatga cacccgccat
     5401 gtcgcccttc aacaacgacg cgaacggctc cttttccgcc cactcggggt gttcgggcgc
     5461 catcgccgtg acttggtcga acgcgaaggc gagctggaag tagatcggct gctccgccca
     5521 cagtgtcccg tcgttgtcga acacggcgac gcgctcggcg ggcgccacgt agtccggtcc
     5581 gccgtccgtg gtgacgcggc gcacgaagtc cattatcgcc cgcttgctcg ctgtctcgtt
     5641 ccacgagggc agcggatcga ccgtcgcctg ctcgaccaat tcgggcgcgc gctggcagct
     5701 cgccgccacg agcaaggccg gcaccagcgc aagacgaagc aggattgttc tcatggctac
     5761 aaatccgcga aggcgtgctt gcgcttgagc tgcagcacgc gcacgtcggg gtcgacgacg
     5821 actttctcgg ggtcgaagtc gcagtggatg gtgagtgtct tcgactcgcc cgcgcccagc
     5881 gtcaccacgc cgcgcgcgtc ctgaaagtcc ggattggcgg tgtaggcgcc gtcggtcgaa
     5941 tcgggctcgt tccatcgctc gccggcggtc gcggcgactt cgaccggcat gacgccggta
     6001 ccgacattct ccaccgtgac ggtgacgtcg aacccgtcgc cgttggcaac cttggaagcg
     6061 ctcgcgacgc ggtactcggg aaccaccttg ccctcgaacc actgcttggt aaaggcgtcg
     6121 taggcggtgg cgtcctccgc gaagggccgc atggccgcca cgaagtcctg cagcgccggg
     6181 tggtcgcggc tcgtgctcca ggtttggaaa aagttgcgat acgcggtccg ggctctttcc
     6241 acgcccatga aatcgtagag catccaaaac acccaaccgc cgcggtcgta gatgatcgtg
     6301 ttatccgagt cgcgcttgcc gtcgatgtcg tacatgggac gctcgtcgtc ggcgcggcgg
     6361 cggttgttgt agcgcgtctc gattcccttc gcgaactcca tgcgcgcgcg cggccccttg
     6421 acctgctcga agagaagcag cgtcgagaag tgcgccgtgc cttcggccag gatgtcgccg
     6481 cccgggccgt tcgccggtgt caggatgtta ccccaccact ggtgggcggc ttcgtgcgcg
     6541 gtgacgagga agctcgcgtc ggtcttggcg tcgttcttgg tcaggaagcc gatgttctcg
     6601 ctgaacgtga tgttggtccc gaagccttgg gcgtagttcg cgagccccgg gaactccgag
     6661 agcttgagct cgcgccacgg atacggcgtg aaccactccg agtaccatgt ccgcgatgcg
     6721 tccagcacct tcgacatctc gtcgacgttg tacgcgtgcg cggggtgata gtaaaccgtg
     6781 ctagactcac cctgcttcgc ttcccagcgc ccgcacacga tgttgagaat cttcaccggg
     6841 tagtcggtct cccacacctg ggtgcgcatg ccattctcga ccgtattgct cgtgcacacg
     6901 ccgaccgagt tgagcgtgta ctcctccggc cccgtgacgg tgatgcgggc cgggaaccat
     6961 gcggtcgctc cgtagctcgc gcgcgtgatg cccttccaat agtcgcgcgg gtactggcgc
     7021 ggttcggtgc gattctcctt cgtctcccct acctgctctt ggaagccaat caccggcatg
     7081 aagctgggac tgaaaccggt cagcaccacg cccgacggga gaatgaactc ccgcgtgctg
     7141 ccgcctttgc gcgtgatccc gcccggatag cgcccgtcga agctccagcc gatgacgacg
     7201 ctgtcaccct tcgcgagggg ctgcgggggc gtgaatagaa acaggagctg gctgtcgtcg
     7261 ggggtgtacg cctcgccgtt catcgtccac gcgaggttct tccagtgacg cgcgcccgtg
     7321 agcggaattt tcacgagtgt ggtgtcgtgc gggttgacga gggtcacaga ccccttgctg
     7381 gagagcgagt gtcgctcggg gtcgagcgcc accgcgacgt cgacgcgcgc gaggtcgggc
     7441 acgggcgcgt cgagccaggt cttgaggttc ttcgcccagt agttcttctc cgccttcttg
     7501 gcggcgccgc cttcgatgcc ctgactcacg tgataggcga gcgcgatcgc cgcgaccagc
     7561 ggtaccgcgg cgtacggcag cagtcgcgcc ccggtgcgaa gcacgcggcg cggcgcgagc
     7621 gcgtgcatgg tgcgaacggc gtccgcaccg cggcgcgcga agagcttcac cgcgaccacc
     7681 gtgaagaaca ccgcgcagcc gaggacgaac aaccgattca gcacgatggc aacgcgatcg
     7741 gtttcgaaga atcccaagtc actccagcgc acggcacccc acagcatcca gttgccggcc
     7801 cagttgatct tgtcggtcag ggcgcggtag ccggtgagaa tcaggagcgc gaggctcaat
     7861 gcatacgcgc cgtagcgatt cccgaccgcc gcgtacgccg cggtgacgaa cgccgtccag
     7921 cccacgaagg tcggaatcaa gagcagtccc cacaacaaca gatacggcgt cagcgcgaac
     7981 ggaaccgtgc cctggatcgc gagcgcgatc gcgcacgcgg ccagcgatgc gagcagcacg
     8041 atcaggacga ccactccgtt cgccagcgct ttgccgatca ataaggccgc ggtgcgcacc
     8101 ggggtcgcgt acaggatcgg cgcgaagccg gtggtgcgct cgcgctcgag cgcctccacc
     8161 gtgtagaaca tcaacaagag acacacgaag gtggaggcct gattcgccag cgtcgccgcc
     8221 gtggtccccg gcgtcagcag caagggggtg tcgaacgccc ccaccgcgaa caacacgttg
     8281 cccagcgact ggagcagaat cagcggcacg aaaatgtaca gcccggcctg gcgtcgcagc
     8341 tcgcgcagct ccgcgccggc cacgcggcgc acggtcgcga cgaacgacgg cgtcccgctc
     8401 ttcattccca gggccagcag cgcgcccgcg cgcgcttctt cccactcgct cgcctccgcc
     8461 accttgcggc gcttgcgcgc ggcgcgtttg gacaccaccg cgccgcggat cgactgctcc
     8521 accgagcgca cggtcaggac caccgcgccc acgcccacca agagcagcca caaccggttc
     8581 aaaccgaaca ggccgtcgta gggcacgcgc tgcgtgttgt aatagtccac cccgcggtcg
     8641 accttcaagt gcgtctcgtt gagccagcga taccccgacg gctcgatcac ctgcaggacc
     8701 tgattgaccc gcaggtcgag ccacgacggc gaccacgtcc acagaaagaa tccggagacc
     8761 aacaacgtgg cgaccggaag tacgaacacc aagacggcgt tgcgcgtcct ctcgccgatc
     8821 accatcgaca tgccggtgaa gaagaccagg aacggcaacc cgatcgtgac caccgggatc
     8881 gcgtagttcg cgaaatggaa cgggccgcgc atctcgtgcg cgtccgcgtt ggggaccgcg
     8941 tggttgaaga atgcggtcgc cgcgatttgc agacccatca acacgacgaa gctcgcgatc
     9001 acggcgagga agcggcccca cacgtactcc gccggtgtca gaggcgtcga gtgcaggagg
     9061 acgtcgacct tgacttcgcg gtcgcggagc agcgtcagcc cggccgccac ggcgatgaag
     9121 aacgcgtagt acaagaggac gaggtacgtc atggtctggg tttgcgcgaa ttcggacgtg
     9181 atccacgcct ttttgccgcc gaccgaggtt tcccccgacg agatttgcat gtgcccgctg
     9241 gcgaggccaa acgcggtgag aatgacgatg agcgccagca tcgcgaacag cggccggcgg
     9301 aaggagtgcc agaactcgac ggagaaggaa gcgtagcagc gccggatgtt catcgagcgc
     9361 cacctgcgtg cacgccgccg gcgtcgatgc cgcgcagctc gcccagccgc atgagcacca
     9421 gatacgcgtc ttcgagcgtc accggcacgg gaacgaaccc gtgcggcggc gatcccttgg
     9481 gctcgtggat gcgggcgcgg ttgcggcctt cgaccaggag tgcctgcgtg acggtgcgct
     9541 cgctgcgcag gcgcgggagg tcggcgacgt cgaccgtccc ttcgtagatg tgcccttcga
     9601 tggcggcgcg cgcctcgctc ggtgtcgtga gcgcgatcaa gcgcccctcg cgaatcaccg
     9661 cgaagcgcgg gcagagcacc gcgacgtcct cgacgatgtg ggtcgagagc agcacgatgc
     9721 gatcggccgc gagctcggag aggagttgat agaagcgcag gcgctcttcc ggatcgaggc
     9781 cggcggtggg ttcgtcgacg atgatcaagc gcggatcgcc ggcgatcgcc tgcgcgatgc
     9841 cgaggcgctg gcgcatgccg ccggagtagc ccttgaccgc gcgcttggcg gcgaaggtga
     9901 gattgacgcg atcgaggagc gcggccacca gcttcttggt gccttgcggc gcttcgacgc
     9961 ccttcaaggc gagcaagtgc gcgagcatcg cctcgccgct caagtgcggg tagaagccga
    10021 attcctgcgg gaggtagccc aggcgcggcc acagccactt ggggtcggtg agcacgtcga
    10081 cgccgtcgag agtgacgctc cccgaggttg gctcgaggag ccccgcgagg atcttcatga
    10141 aggtcgactt gccggcgcca ttgggtccca ggaggccgaa catccccggc gagacgtcga
    10201 ggtcgatgcc gttgagcgcc gtgacgggac cgggatagat cttggtgagc gcgcgagcgg
    10261 atagcacggg gacctcctcg tcagagtggg gatgccggcc gcgtgaccca gggccgaagg
    10321 cgcggcggct cgacgggaaa acgaaagtac ctacgcggca cccgacggcg aggttgcgcg
    10381 gccgcgttca cggcggcgtc atctttccac ccgctgcacc tcgggccgct tccacgtccc
    10441 gtcgtaggcg gccggcttcg gcccgtacag gcgcagcacc atcatgaacg gaccggccgg
    10501 ggcgggcagc cagttggttt gcttgcccgg tcccggtgac gcgtactgca tgtagagggt
    10561 caacccgccg tccttgtcct tgcgcagctt ggggagcatg ggcgagttga tgagatagcg
    10621 ctcgatcgaa ttggggacca agagcttggt cttcgcgtcg tacatcgtga gtgaccagaa
    10681 tgcgctaacg ggcgggagct ggttgggaga gaaacgcagc gtgtagttcc ccaccgtccc
    10741 gtccagcgct tgcccgcccg aatcgacgcc gtacccgaaa tagagcgctt cttctttgga
    10801 gttgccgtag attccgtaga ccgccgcggt catgcgatag agatagttgt cggccaggct
    10861 ctcgcgggtg ccgaacaagt cgccgctgga gatctggccc gcgatcagca gctgattcac
    10921 cgagtcggac gccgcccacg cgtccgcgcg accgtcttcg agcgccttgc ggatttcggg
    10981 cttccgcgcg gcggcgtcga aggagagccc cgcgcccacg ccgatcttcg cgaagcgcgc
    11041 catcagctcc ccttcggtcg ggtgcgtcgg gcagaactgc agcacgaaat tcagaatgcc
    11101 gaagaactcg agcgacgtac gctcttcttg ggcgctgagc ggcttcacga atgcgatggg
    11161 cggcgcgggt tcgggcgcgg gggtcccgag gaaccgcgac aacggctgga cccggtagcc
    11221 gatttggact tcctcgacgt tcttgatgtc cttcgggccg aacaactgcg ttcgatacgc
    11281 gaccatcgcc agctcggtct cgcaccgaat cacctgcgtg atcccgggcg gggtttcccc
    11341 ctgccagccc gggcccgcga gcaggaagtt cccgccctcg ctgccggtcg cacggcttcc
    11401 cgcatacgcg aagttgaacg tgtagaggtc gatgaactgc gccgaatagt agcgtccctt
    11461 ctccaccgcg gggagggtga gcaccagcgg ctcggcgcgc aaatcggcgc ccaacatcga
    11521 gtagggcgtg tcggagttcg gcgtttgcac cgccttgtcg tccggcgtaa acacgcgcgc
    11581 gatactcctg atgcggttcc aatcctcttt gaactcggga ttggtgccgt cgacgaagta
    11641 cgcgtgcatg atgcggtagc tgtcgaccat cgggaagccg tagatgtagg cctccttcgc
    11701 gatcgctcgt acctccgcgg gcgtgatcgc ttccttcgac gagcacgacg ccagtgtggc
    11761 gccggcgaag atcgtaacca gtacgcgcgc gaccttcatg acaccgttcc tttcgttgtg
    11821 gggtccacat gctaaacgac cccgggcgga atgtccatcg cccggggtcg aaatggcgta
    11881 cgtacgcgat gttcgttagg ccgcggtggc cagttcgtcg ccgttctcgt cgacggcccg
    11941 tgcgaccgtg gcgcgccgcg tcttggcgac cagcacgtag atggcgggca cgaagaagag
    12001 cgtgaagacg gagccgatga tcatgccgct cacgagcatg ataccgatcg agttgcgcgc
    12061 ccccgcgccg ggaccgtgcg cgaacacgag cggcaagtgc cccatcacgg tcgcggcggt
    12121 ggtcatcagg atgggccgca atcgcgtgcc cgccgcttcg atcacggcgg tcgtcttgtc
    12181 gcgcccctcc atttgtaagt ggttcgcgaa ctcgacgatc agaatgccgt tcttcgccac
    12241 cagccccacc agcgtgatga gcccgacctg gctgtagata ttgagcgtcg tgaacccgag
    12301 gaacgaaaag atcaacgatc ccgaaagggc cagcggcacc gaccccgcga gaatcacgaa
    12361 cgggtcgcgg aagctctcga actgtgcggc cagcaccagg taaatgagaa tcgccgagag
//
LOCUS       DASPGK010000347         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1139157, whole genome shotgun sequence.
ACCESSION   DASPGK010000347 DASPGK010000000
VERSION     DASPGK010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139157"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2554)
                     /locus_tag="VEC56_09735"
     CDS             complement(17..2554)
                     /locus_tag="VEC56_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXV14473.1"
                     /translation="MRFADRLAAACISVGGVGTILAVSAVFLFLASVVVPLFTRGDTE
                     PVATMPLDGSKSARRALHVGTDEYRVLGWSLDADGVVRVFRADTGDSVGTMRIAAADS
                     LVAASFDVASRSFALGLSDGSLAFGRVVFRTDFPDPAAVAQAASLAPGAAMVVDGAVV
                     QMTPDGALRRQAPAAPVEVRVPSVVRGAVRAVDHIVIDSGYLVAALGDGDTVSVCHVA
                     RSENEFTGEVTLSVRRGVLVPLAPGAAAPERVVVAEAGSIVYVVDAAGRATVWDTRDV
                     TSPTVAATAQLVDSGRRVTALAPLLGRATILVGSDDGAISTWFRYAPEAGTGETPELV
                     SALAPAQHFAGAGAPVTALSASARERVFVAGYGDGRVRVLQATSGKRVVDFQAGDSPV
                     TAAAFAPKSDGLVLLDASAFRSYDLDLGHPEVTVRSLFRPMWYEGYPRAEHVWQSSAG
                     TDDFEPKLGLWPLVFGTLKATFYSMLFGVPLALLAAIFTSEFVSPGARPRLKTLIESM
                     ASLPSVVLGFLAALVIAPFVQRAVPSVIVGFIAIPAVLLTAAYVWQTIPQTTVRRLDR
                     FRLGIALATLPLAVLIAAAGGPWMERAFFGGDMMGWLAGGPGGVFGGWFVLTLPVCAF
                     VAVFVMGRLTGRAIRDLASRSTRSRVAGFDFARFATGALLTIGLAAGFAAVLAALGAD
                     PRGGLMGTYVQRNALVVGFVMGFAIIPIIYTIAEDALSAVPDHLRAASLGAGATPWQT
                     AIRIVLPTAMSGLFSAVMIGLGRAVGETMIVLMAAGNTPVLEMNAFSGFRTLSANIAV
                     ELPEAVRNSTHYRTLFLAALLLFFMTFVINTVAEIVRMRFRKRSAQI"
     gene            complement(2688..>3435)
                     /locus_tag="VEC56_09740"
     CDS             complement(2688..>3435)
                     /locus_tag="VEC56_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV14474.1"
                     /translation="APPALIAGTATFGPMSREMKNQEIDEFEAKYGYKPTGLRTSIDM
                     LAVYVHKDNPIESLTMQQIDAMFSKTRKGGHKEDITTWGQLGLTGEWANKPISLYGRN
                     SASGTYGYFKEHALFKGDYKDGVKEQPGSSSVVQGVASDRYGIGYSGIGYKTADVKAV
                     PIKADAGSEAIPATPEFAYTGKYPLARFLYVYVNHKPGSELDPLRREFIRFMFSKQGQ
                     ESVVKDGYYPLNSKIAGEELAKLGIQVSSR"
BASE COUNT          606 a         1199 c         1158 g          472 t
ORIGIN      
        1 gatgcgaccg gcgtgctcat atctgcgccg acctcttccg gaatcgcatg cgcacgatct
       61 ccgcgaccgt gttgatgacg aaggtcatga agaacaggag caaggcggcc aggaacagcg
      121 tgcggtagtg cgtgctgttg cgcaccgctt cgggaagctc gaccgcgatg ttggcggaaa
      181 gcgtgcggaa gccgctgaac gcgttcatct cgagcacggg cgtgttgccc gccgccatca
      241 gaacgatcat ggtttcgccc acggcgcgcc ccagcccgat cataacggcg gagaacaggc
      301 cgctcatcgc cgtcggcagc acgatgcgaa tcgcggtctg ccacggtgtc gcgcccgcgc
      361 ccagcgacgc ggcgcgcagg tgatccggca ccgcggacag cgcgtcctcc gcgatggtat
      421 agatgatggg aatgatggcg aagcccatca cgaatcccac caccaacgcg ttgcgctgca
      481 cataggttcc catcaggccg ccgcgcggat cggcgcccag ggccgcaagc acggccgcga
      541 acccggccgc caacccgatc gtgagcaggg ccccggtcgc gaagcgcgcg aagtcgaagc
      601 cggccacccg cgaacgcgtc gagcgagacg ccaagtcgcg aatcgcgcgg cccgtcagcc
      661 ggcccatgac gaacacggcg acgaacgcgc acaccggaag cgtgagcacg aaccagccgc
      721 cgaacacgcc gccgggtccg ccggccagcc agcccatcat atcgccgccg aagaacgcgc
      781 gctccatcca cggtccgccg gccgccgcga tcagcaccgc gagcggcagc gtcgccagcg
      841 cgatccccag gcggaagcga tccagccgtc gcaccgtggt ttgcgggatg gtctgccaga
      901 cgtacgccgc cgtgagcagc acggcgggga tcgcgatgaa gccgacgatc accgacggga
      961 ccgcgcgctg cacgaaggga gcgatcacca gcgcggccag gaagcccagc acgacgctgg
     1021 ggagactcgc catcgactcg atcaatgtct tcagacgggg gcgcgcgccg ggagagacga
     1081 actcgctggt gaagatcgcg gccaacaacg ccagcgggac gccgaaaagc atcgagtaga
     1141 acgtcgcctt gagggttccg aacacgagcg gccacaaccc gagcttgggt tcgaagtcgt
     1201 ccgtgcccgc cgacgactgc cacacgtgct ccgccctcgg atagccctcg taccacatgg
     1261 gtcgaaagag ggagcgcacc gtcacttcgg ggtgcccaag gtcgaggtcg taggagcgga
     1321 aagcgctcgc gtcgagcagc accagcccgt cgctcttggg cgcgaaggcg gccgcggtca
     1381 cgggcgaatc gccggcctgg aagtcgacca cgcgcttgcc gctcgtcgcc tgcaggacgc
     1441 gcacgcgacc gtcgccgtag ccggctacga acacgcgctc gcgcgcggag gccgagagcg
     1501 cggtgacggg cgcgccggcg ccggcgaagt gctgggcggg ggcaagcgcg gaaacgagct
     1561 cgggggtttc gccggtaccg gcctcgggcg cgtagcgaaa ccacgtcgat atggcgccgt
     1621 cgtcggagcc cacgaggatg gtcgcgcgcc ccagcagcgg tgccagagcg gtgacgcgcc
     1681 gtcccgaatc caccagctgc gcggtggccg ccacggtggg ggaggtgacg tcgcgcgtgt
     1741 cccaaaccgt tgcgcgcccc gcggcgtcga cgacgtacac gatcgagccc gcttccgcga
     1801 cgaccacgcg ctcgggcgcc gctgcaccgg gcgccagcgg aaccagcacg ccgcggcgga
     1861 ccgacagagt cacttcgccg gtgaactcgt tctcgctacg ggcgacgtgg cagaccgaca
     1921 cggtgtcgcc gtcgccgagt gccgcgacca gatagccgga gtcgatcacg atgtggtcga
     1981 cggcgcgcac cgcacctcgc accacggagg gaacgcggac ctcgaccggc gcggccggcg
     2041 cctggcggcg cagcgcgccg tcgggcgtca tctgcacgac ggcgccgtcg acgaccatcg
     2101 cggcgccggg cgcgagcgaa gccgcctgcg cgaccgccgc cgggtccgga aagtcggtgc
     2161 gaaacacgac gcgcccgaac gcgagcgatc cgtcggagag gccgagcgcg aaggagcgcg
     2221 acgcgacgtc gaacgacgcg gcgaccagcg agtccgcggc agcgatgcgc atcgtcccca
     2281 cgctgtcgcc ggtatcggcg cggaacacgc gcaccacgcc gtccgcatcg agtgaccacc
     2341 ccagaacgcg gtactcgtcg gtgcccacgt gcagcgcccg gcgcgcgctc ttcgacccgt
     2401 cgaggggcat cgtcgcaacc ggctcggtgt cgccgcgcgt gaagagcggg accaccacgc
     2461 tggccaggaa gagaaacacc gcggaaacgg ccaggatggt cccgactccg ccgaccgaaa
     2521 tgcacgccgc ggccagccgg tccgcgaatc tcacggccca gcgcgtgcgg cgattacgac
     2581 cgcggcgttc taccttgcgt gatggcgcca tattggaacg cacgagtggg cgacgccgaa
     2641 atcgacgtcg cccaccgcac cgaaactact tctcctcgaa accgagacta tcgactgctg
     2701 acctgaatgc ccagcttggc gagctcttcg cccgcgatct tcgagttgag cgggtagtag
     2761 ccgtccttga ctacgctctc ctgcccctgc ttgctgaaca tgaagcgaat gaattcccgg
     2821 cgcagcgggt ccagctcgga ccccggcttg tgattgacgt acacgtagag gaagcgcgcg
     2881 agcgggtact tgcccgtgta cgcaaactcc ggcgtggccg ggatggcttc gctgccggcg
     2941 tcggccttga tgggcacggc cttcacgtcc gcggtcttgt aaccgatgcc gctgtaaccg
     3001 atgccgtagc gatcactcgc gacaccctgc accaccgacg agctgccggg ctgctccttg
     3061 acgccgtcct tgtagtcgcc cttgaagagc gcgtgctcct tgaagtaacc gtacgtgccc
     3121 gacgcggaat tgcggccgta caagctgatc ggcttgttgg cccattcacc cgtcagtccg
     3181 agttgtcccc aggtcgtgat gtcttccttg tggccaccct tgcgcgtctt cgaaaacatc
     3241 gcgtcgatct gctgcatggt cagcgactcg atcgggttgt ctttgtgcac gtagaccgcg
     3301 agcatgtcga tcgacgtgcg caggcccgtg ggcttgtagc cgtacttggc ctcgaactcg
     3361 tcgatctcct ggttcttcat ctcgcggctc atgggaccga aggtggccgt tcccgcgatc
     3421 agcgccggcg gcgcc
//
LOCUS       DASPGK010000348         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_86025, whole genome shotgun sequence.
ACCESSION   DASPGK010000348 DASPGK010000000
VERSION     DASPGK010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86025"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VEC56_09745"
     CDS             <1..422
                     /locus_tag="VEC56_09745"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXV14475.1"
                     /translation="EKGVAREQARKDLPLSTYTEAYWKIDLHNLLHFLALRMEDHSQL
                     EIRNYAKRIGEDIVAKWVPFTWEAFIDYRMNSVALSAIEREVVRAVAKGDSALARKIV
                     EERGWLEPATSGGWKRNREREELESKLSDLNLAVPWL"
     gene            437..844
                     /locus_tag="VEC56_09750"
     CDS             437..844
                     /locus_tag="VEC56_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HXV14476.1"
                     /translation="MQGTFQLVEPGALRPHEDVDRERVRALSEEIRSAGSFYPPVLID
                     QSTRVILDGHHRWHASALLGFALLPCYAVDYLHDDAIRVISRRTEIAVTKHDVLAMAL
                     SGRVYPCKTTRHVYDLPEAIEPVPLDRLLHATL"
     gene            958..1563
                     /locus_tag="VEC56_09755"
     CDS             958..1563
                     /locus_tag="VEC56_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HXV14477.1"
                     /translation="MKSYVHALLLAAIVACGMGCSGGKQVTRIDPDETVDLSGRWNDT
                     DSRLVSETMISDCMSRPWKANHIDKKGKRPTIIVGQIRNKSSEHIAVGTFIGDIERAF
                     INSGEAEMVASAEEREQIRGERADQQQYSSEETMKQWGREHGADYMLSGVINSIEDSE
                     DGKAIVFYQVDLTLIDLESNAKVWAGQKKIKKFIGRGDYKL"
     gene            1570..2946
                     /locus_tag="VEC56_09760"
     CDS             1570..2946
                     /locus_tag="VEC56_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14478.1"
                     /translation="MTSGGWTRPTALVGALVVLACVTACASYVSQSGQIRDALMADDY
                     DRALKTIEDISQSNAKLLYLYEKGLVLHCRGDFAESSKAFTESELVLEDLYTRSITRE
                     VASMTVSETISQYRGDAFEAVYVNYYQILNYLGLRDLDGAMVECRRVNRKLQMLHDGG
                     ETYFTNDPFVQYLTGLVYQMGGEREAAGVSYRVAAELYDGGGFAPVAPVPPSFFCDAA
                     DNAFTRGDREEEAAYAARARCAVGADTGHVSVLIECGQIVRKRENSVVLPIFESDRWT
                     DNEKFAHELSQRHGVHYGPQVRVKYWLKVALPALELMPPRYGGVVVRARPVEKGNAAE
                     THATVVSNLDALAEQAFREKESTMFVRAIVRAIVKYLAHDAADDKDEGFGTLVNIFNV
                     VTETADTRSWSSLPSRIYLARLDLPAGRYRVEVDLLGPDDRRARSLVFDDVTVPGGGH
                     VIRNARAF"
     gene            2991..>4919
                     /locus_tag="VEC56_09765"
     CDS             2991..>4919
                     /locus_tag="VEC56_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXV14479.1"
                     /translation="MGSAVRLCIALLLAFLASCGDKSRRSEVPMGQNRLALEQSPYLR
                     QHADNPVDWYPWGDEAFAKARAEDKPIFLSIGYSTCHWCHVMEHESFEDAEVAALMNR
                     AFVCIKVDREERPDVDHVYMTVCQMMTGSGGWPLTLLLTPERRPFFAATYIPKAAMLS
                     FIPRVEAAWRDNRDSVLTDSERVTRALASAVTPEAGDELGLDTMNDAYRGFVASFDST
                     YGGFGARPKFPTPHTLMFLLRHWKRTGDAGALAMVEKTLDGMRRGGLFDQVGFGFHRY
                     STDAQWLLPHFEKMLYDQALLLLAYTEAYHATRNDAYRRTAEEIAAYVMRDLSSPEGG
                     FYSAEDADSEGEEGKFYVWTLAQLREVLGADAGFAALAFGVTEDGNFAEEASGHRSGA
                     SVLYVPKPQPEAARAAGLAEDAFAERVESVRARLFEARARRVRPHLDDKVLTDWNGLM
                     IAALARSGRVLDRPDHIERARLAARFLRERLTGKGGALLHRYRDGDAAIAGMLDDYAF
                     LAWGLLELYEATFDVAHLSEAIRLTRRMDERFAAPGGGYFMTEKGSDDLIVRTREIYD
                     GATPSGNSVAMLNLARIARFTGEMQWDRKARAVGSAFASQASRLPMAHAFAMAAVDFL
                     AGPTFEVVVAGARGRPDTN"
BASE COUNT          850 a         1624 c         1646 g          799 t
ORIGIN      
        1 tcgagaaggg cgtggcccgc gagcaggcgc gcaaggacct gccgctgtcc acgtacacgg
       61 aggcctactg gaagatcgac ctccataact tgctccactt cctggcgctg cgcatggaag
      121 accactcgca gctcgagatt cgcaactacg cgaagcgcat cggtgaagat atcgtcgcca
      181 agtgggtgcc cttcacgtgg gaggcgttca tcgactatcg catgaactcg gtggcactct
      241 ccgcgatcga gcgtgaggtc gtgcgcgcgg tggccaaggg cgattcggcg ctcgcgcgca
      301 agatcgtgga agagcgcggc tggctcgagc ccgcgaccag cggcgggtgg aagcgcaacc
      361 gcgagcgcga agagctcgag agcaagctgt ccgacctcaa cctcgccgtt ccctggctgt
      421 agacgcgcgt cccgccgtgc agggcacgtt tcaactcgtc gaacccggcg ccctgcgacc
      481 gcacgaagac gtggacaggg agcgcgtgcg cgcgctctcg gaggagatac gctccgcggg
      541 cagcttctac ccccccgtcc tgatcgacca gtcgacgcgc gtcatcctcg acggccacca
      601 ccgctggcac gcgtcggcgc tgctgggatt cgcgttgctc ccgtgctacg ccgtcgacta
      661 cctgcacgac gacgccatcc gcgtcatctc gcgccgcacc gaaatcgccg tgaccaaaca
      721 cgacgtgctc gcgatggcgc tctccggccg cgtctatccc tgcaagacca cgcgccacgt
      781 gtacgacttg cccgaggcga tcgaaccggt gcccctggac cgcctcctgc acgccaccct
      841 ctagccgcct cttcggttga caacccggcg ccgaacgacg cactctctcg tcgacgcgct
      901 tcgcagtcga cgacgctccc ggcgggtctg gtccggggaa cgaaggagga tatcggtatg
      961 aaaagctacg tgcacgcgct gctcttggcc gcgatcgtgg cctgcggcat ggggtgttcg
     1021 ggaggaaaac aggttacgcg catcgacccc gacgagaccg tcgacctctc cggtcgctgg
     1081 aacgacaccg actcgcgtct ggtgtccgag accatgatct ccgactgcat gtcgcgcccg
     1141 tggaaggcga accacatcga caagaagggc aagcggccga ccatcatcgt cgggcaaatt
     1201 cgcaacaagt cgagcgagca catcgcggtc gggaccttca tcggcgacat cgagcgcgcg
     1261 ttcatcaact cgggcgaggc cgagatggtg gcgagcgcgg aggagcgcga acagattcgc
     1321 ggcgagcgcg cggaccagca gcagtattcg tccgaggaga cgatgaagca gtggggacgc
     1381 gagcacggcg ccgactacat gctctccggt gtcatcaata gcatcgagga ctccgaggac
     1441 ggcaaggcga tcgtattcta ccaagtggat ctcacgctga tcgatctgga gtcgaacgcg
     1501 aaggtctggg cgggccagaa gaagatcaag aagttcatcg gccggggcga ttacaagctc
     1561 taacccggca tgacctccgg gggttggact cggccgacgg cactcgtcgg ggcgctggtc
     1621 gtgctcgcgt gcgtcacggc ctgtgcgagc tatgtctctc agtcgggcca gatccgcgac
     1681 gcgctcatgg ccgacgacta cgaccgtgcg ctcaagacga tcgaagacat cagccaatcc
     1741 aacgcgaagc tgctctattt gtacgagaag gggttggtcc tacactgtcg cggggacttc
     1801 gcggagtcga gtaaggcgtt cacggaatcg gaactggtgc tcgaggacct gtatacgagg
     1861 agcatcaccc gtgaagtggc atcgatgaca gtctcggaaa ccatctcgca gtaccgcggc
     1921 gacgcgttcg aagcggtcta cgtcaactat taccagattc tcaactacct cggcctccgc
     1981 gacctcgacg gcgccatggt cgagtgtcgg cgcgtcaatc gcaagttgca gatgctccac
     2041 gacggcggcg agacctactt caccaacgac cccttcgtcc agtacctgac cggcctggtc
     2101 tatcagatgg gcggggagcg cgaggcggcg ggcgtgtcct accgcgtggc ggccgagctc
     2161 tacgacggcg gcggattcgc gcccgtggcg ccggtcccgc cgtctttctt ctgcgacgcg
     2221 gccgacaacg cgttcacgcg cggcgaccgc gaggaggagg ccgcgtacgc ggcgcgggcg
     2281 cggtgcgcgg tcggcgccga caccggccac gtgtcggtac tgatcgaatg cgggcaaatc
     2341 gttcgcaagc gtgaaaacag cgtggtgctc ccgatcttcg agagcgatcg ttggaccgac
     2401 aacgagaagt tcgcgcacga actctcgcaa cggcacggcg tgcactacgg cccgcaggtg
     2461 cgcgtcaagt actggctgaa ggtcgcgctg ccggcgctcg aactaatgcc cccgcgctac
     2521 gggggcgtgg tggtgcgcgc gcggcccgtc gagaagggga acgccgcgga gacgcacgcg
     2581 acggtggtgt cgaacctcga cgcactcgcc gagcaagcgt ttcgggagaa ggaatcgacc
     2641 atgttcgtgc gcgccatcgt gcgcgccatc gtgaagtacc tcgcgcacga cgccgccgac
     2701 gacaaggacg aaggtttcgg cacgctcgtg aacatcttca acgttgtcac cgagaccgcg
     2761 gatacgcgca gctggtcgtc gctccccagt cggatttatc tcgcgcgcct cgacctgccg
     2821 gctgggcgct accgggtcga agtcgatctc ctcgggccgg acgatcgccg cgcgcgatcg
     2881 ctggtgttcg acgacgttac cgtgcccgga ggcggccacg tcattcgcaa cgcgcgcgcc
     2941 ttctgaacgt gctatggtcg gagcggccgc cgacgtcgcg gccgcgcacc atgggttccg
     3001 ccgttcgctt gtgcatcgca ttgctgctcg ccttcctcgc ttcctgcggg gacaagtcgc
     3061 gccgctccga ggtcccgatg ggtcagaacc gcctggcact cgaacagagc ccgtaccttc
     3121 gccagcacgc cgacaacccc gtcgattggt atccgtgggg cgacgaggcg ttcgcgaaag
     3181 cgcgcgctga agacaagccg atctttctgt ccatcggcta ctcgacctgc cactggtgtc
     3241 acgtcatgga gcacgagtcg ttcgaggatg ccgaagtggc ggcgctcatg aaccgcgcct
     3301 tcgtgtgcat caaggtcgac cgcgaagagc gccccgacgt ggatcacgtg tacatgacgg
     3361 tgtgccagat gatgacgggg agtggcggct ggccgctgac cctcttgctc acgcccgagc
     3421 gccggccctt cttcgcggcg acctacattc ccaaggccgc catgctgtcg ttcatcccgc
     3481 gcgtcgaagc ggcgtggcgc gacaatcgcg actcggtgct cacggattcg gaacgcgtga
     3541 cgcgcgcgct cgcgagcgcg gtgacaccgg aagcgggcga cgagctcggg ttggatacga
     3601 tgaacgacgc ctatcgcggt ttcgtcgcca gcttcgactc cacctacgga ggcttcggcg
     3661 cgcgcccgaa gttccccacg ccgcacactc tcatgttcct gctgcgccac tggaagcgga
     3721 ccggcgacgc gggggcgctc gcgatggtcg agaagacgct cgatggcatg cggcgcggcg
     3781 gcctgttcga tcaagtgggt ttcggtttcc accgctactc gaccgacgcg cagtggctgc
     3841 tgccgcactt cgagaagatg ctctacgacc aggcgctgct cttgctcgcg tacaccgagg
     3901 cgtaccacgc cacgcgcaac gacgcgtacc gacgcaccgc ggaggagatc gcggcctacg
     3961 tcatgcgcga tctctcctcg ccggagggcg gtttctactc ggccgaagac gccgacagcg
     4021 aaggcgaaga gggcaagttc tacgtgtgga cgctcgcgca gctgcgcgag gtactgggag
     4081 cggacgcggg cttcgcggcg ctcgcgttcg gcgtcaccga ggacggcaac ttcgcggaag
     4141 aggcgagcgg ccaccggtcg ggtgcgagcg tcttgtatgt gcccaaaccg cagccggaag
     4201 ccgcgcgtgc cgccgggctg gccgaagacg ccttcgccga gcgcgtggag tcggtgcggg
     4261 cgcgattgtt cgaagcgcgc gcgcgccggg ttcgccccca tctcgacgac aaggtattga
     4321 ccgactggaa cggcctcatg atcgcggcct tggcccgcag tggtcgcgtg ctcgatcgcc
     4381 ccgatcatat cgaacgcgcg cgcctcgccg cgcgcttcct gcgcgagcgg ctgaccggca
     4441 agggcggcgc gctgttgcat cgctaccgcg acggtgacgc ggccatcgcc gggatgctcg
     4501 acgactacgc gttcctggcg tggggtctat tggagttgta cgaagcaacc ttcgacgtcg
     4561 cgcacttgtc ggaagcgatt cggctcacgc ggcggatgga cgagcgtttc gccgcgccgg
     4621 gcggcggata cttcatgacc gagaagggaa gcgacgacct gatcgtgcgc acccgcgaga
     4681 tctacgacgg cgccacaccc tcgggcaact cggtggccat gctgaacctc gcgcgcatcg
     4741 cgcgtttcac cggcgagatg cagtgggacc gcaaggcgcg cgcggtcggc tcggcgttcg
     4801 cgtcgcaggc ctcgcgcctt cccatggcgc acgcgttcgc gatggcggcg gtcgacttcc
     4861 tggccgggcc gaccttcgag gtggtggtcg cgggcgcgcg cggaaggccc gacacgaac
//
LOCUS       DASPGK010000349         5144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_670914, whole genome shotgun sequence.
ACCESSION   DASPGK010000349 DASPGK010000000
VERSION     DASPGK010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670914"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VEC56_09770"
     CDS             <1..524
                     /locus_tag="VEC56_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HXV14480.1"
                     /translation="VSQYGIPAYDAHVLTGSGDVADYYEAVVAAGADGKSASNWVMGE
                     VLRELNERKIDIGEFGIPAGNLAELATLQASGKVNSTTAKEVFQEMLASGKTAGAIVQ
                     AKGLEQISDDAAIEKEAVAVLDENPDEVTRYLAGKEQLLQFFVGQLMKRTRGKANAQL
                     ATGILKGLLEKRR"
     gene            624..1253
                     /locus_tag="VEC56_09775"
     CDS             624..1253
                     /locus_tag="VEC56_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HXV14481.1"
                     /translation="MKYVAFFLFCLLTVTAFAPEGAAQIRLNEILGDPGTDWNGDGNV
                     DTKLDEWVEIINVGNAAVDLANYRISDASAATEFRFALSGMLGPGEVRVYFGSDVVAW
                     QAANGVGQLGLSLNNAGDTVFLYEVNGTDIAVNDTRTYATAEIANDRSTGRLPQGNGG
                     WVIFDALNPYTGAQPPTPTGCAPSPGQGPQCPTPTHSSTWGKIKALYRG"
     gene            1420..2376
                     /gene="meaB"
                     /locus_tag="VEC56_09780"
     CDS             1420..2376
                     /gene="meaB"
                     /locus_tag="VEC56_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HXV14482.1"
                     /translation="MTQRMTDLPQLLARFGAGDALSASRAMSVIERGGDEAESLLDAI
                     YGKTGRAWRIAFTGPGGAGKSSLVDRVIEKERARNRTIGIVAEDPSSPFSGGAILGDR
                     VRMAHAQGDAGVFVRSLASRGSETGLSALAGELADVMDAFGRDVVILESMGVGQVETR
                     VRYMADTVVVVLTPEAGDDVQTMKSGLLEVADVLVVNKSDRPGADALAADLTAVIGLR
                     TSTKDPWAPPVVQTSATEGRGIDALDAALAGHRAHLDEKGRRAARRREALRERVRLLV
                     EERVREDLWRDPTRAARFDGILEAVVGGRTSPYQAARELALP"
     gene            2373..2777
                     /locus_tag="VEC56_09785"
     CDS             2373..2777
                     /locus_tag="VEC56_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXV14483.1"
                     /translation="MNRPIKVLIAKPGLDGHDRGAKVIANALRDAGMEVVYTGLHQTP
                     EMVVDAALQEDVDLVSVSILSGAHMTLLPRVRELMNTRGLADVKLIAGGIIPDADAEA
                     LKKLPGIAGIFGPGTSTQDVVKFIQSLFTHRK"
     gene            2782..3930
                     /locus_tag="VEC56_09790"
     CDS             2782..3930
                     /locus_tag="VEC56_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV14484.1"
                     /translation="MSQVLLNEEHQMIRETARRFAEAELVPNAAHIDATGEYPVENFK
                     KMGELGFLGLIVPAEYGGTFIDTVSYAIVIEELARACGSTALGVAAHNSLACGPLLLM
                     GSEAQKKRFLPELASGEAIGAFCLTEPQAGSDASNTKTTAVLKGDTYILNGTKIYVTN
                     GGWARYLIATAVTDPGKGSKGISAFVVENDFPGFVVGSREKKLGLRASGTYEIVFDNC
                     EVPKANLLGSEGGGFRTFMKTLEAGRISIGAFSLGIAQAAMEAAIKFGKDRKQFDTPI
                     TEFQATQLKIADMATEIHAARLLIHDAARRKDAGEDFGRYASMAKLFASEMAMRVTDQ
                     AIQIHGGYGYCKEYHVERFYRDAKLGEIGEGTSEIQRIIIARAVLGDE"
     gene            3959..5143
                     /locus_tag="VEC56_09795"
     CDS             3959..5143
                     /locus_tag="VEC56_09795"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXV14485.1"
                     /translation="MERAVIVSALRTPIGRFMGGLSTLPAPRLAAGLIKETLARTKLD
                     SETIDEVILGNVLQAGVGQAPARQAMIFGGVAPSVGAVTINKVCGSGLKSVMLAAQAI
                     KAGDAEVVLAGGMESMSMAPYLLPDARAGQRLGHGRLIDAMINDGLWDCYNDYHMGNT
                     GELVAKKYGISRETQDEFAVESHKKAVAAAERGDFKDEIVPVMVPQPKGEALAVAVDE
                     GPRADASKEKLAKLKPAFEKDGTVTAGNASTINDGASVLLVMTEKKAKELGLEVLAVI
                     DAYATGGMEPQWVMMAPVKAVGKLLERTKTRITDYDVIELNEAFAVQGVALIRELKIP
                     PERLNVNGGAVALGHPIGASGARVLTTLIHAMRKRRAKRGLVSLCLGGGNAVAMSVSL
                     PQ"
BASE COUNT          955 a         1631 c         1765 g          793 t
ORIGIN      
        1 tcgtgtccca atacggcatc cccgcctacg atgcccacgt gctcaccggc tcgggcgacg
       61 tcgccgacta ctacgaagcc gtcgtcgccg ccggcgcgga cgggaagagt gccagcaact
      121 gggtcatggg cgaagtgctg cgcgaattga atgagagaaa gatcgacatc ggcgaattcg
      181 ggatccctgc aggtaatctc gccgagctgg cgaccctgca agcgagcggc aaggtcaact
      241 cgaccaccgc gaaggaggtc ttccaagaga tgctcgcgag cggcaagacc gcgggcgcga
      301 tcgtgcaggc gaagggcctc gagcagatca gcgacgacgc cgccatcgag aaggaggccg
      361 tcgccgtcct cgacgagaac cccgacgagg tgacgcgtta cctcgcgggc aaggaacaat
      421 tgctgcaatt cttcgtcggt cagctcatga agcgcacgcg cggcaaggcg aacgcccaac
      481 tcgcgaccgg gattctcaag ggcctgctcg agaagcggcg ctagccctct tgcgcacggc
      541 ggccccccat gctatagtgg cggccgttag ttcccgccaa ttccaccggc aacggaaggg
      601 gcgtttggga ggaggatgtg aggatgaagt acgttgcctt ttttcttttc tgcttgttga
      661 ccgtaaccgc gttcgcaccg gaaggtgcgg cgcagatccg cttgaacgag attctgggcg
      721 accccggcac cgattggaac ggtgacggga acgtcgacac gaagctcgat gagtgggtcg
      781 aaattatcaa tgtcggtaac gccgcggtcg atctcgcgaa ctaccgcatc tcggacgcct
      841 ccgcggcgac cgagtttcgc ttcgcgctct cgggcatgct gggcccgggc gaagtgcggg
      901 tgtattttgg gtcggacgtg gtggcgtggc aggccgcgaa cggtgtcggg cagttggggt
      961 tgagtctcaa caacgccggc gacaccgtct tcctctacga ggtcaacggc accgacatcg
     1021 cagtgaacga cacgcgcacg tacgcgaccg cggagatcgc gaacgatcgc tcgaccggac
     1081 gcttgccgca aggcaacggc gggtgggtga tattcgacgc gctcaacccg tacacggggg
     1141 cccagccgcc gacaccgacc ggctgtgctc cgtcgccggg ccagggcccg cagtgtccga
     1201 cccccacgca ttcctccacg tggggcaaga tcaaggcgtt gtaccgcggc tagccgcgta
     1261 ggtaactcgt tgtcagagta agtcaatcca cagtgggccc cgacgttgcc ggtggggggg
     1321 cgggagaagg attgacaggg tgaagccgtt caagtaagtt gaagcccgat aatgggttcg
     1381 ggccgtcttg cctcccttgg aggcgaggcg cgccgggctt tgacacaacg catgaccgac
     1441 ctcccgcagc tgctcgctcg cttcggcgcg ggcgacgccc tttcggcgtc gcgcgcgatg
     1501 tcggtcatcg agcgcggggg cgacgaagcc gaatcgttgc tcgatgcgat ctatggaaaa
     1561 accgggcgcg cgtggcgcat tgcgttcacc ggtccgggtg gcgcggggaa gagcagcctg
     1621 gtggatcgtg tgatcgagaa agagcgcgcg cgcaatcgca ccatcggaat cgtggccgaa
     1681 gatccctcga gcccgttttc gggcggagcg attctcggcg accgcgtgcg catggcgcac
     1741 gcgcagggcg acgcgggggt gttcgtgcgc tcgctcgcga gccgcggcag cgagacgggc
     1801 ttgagcgccc tggccggcga gctcgcggat gtgatggacg ccttcggccg cgacgtcgtg
     1861 atcctcgaaa gcatgggtgt gggccaggtg gagacgcgcg tgcgctacat ggccgacacg
     1921 gtcgtggtcg tgctgacgcc ggaggcggga gacgacgtgc agaccatgaa gtcgggcctg
     1981 ctcgaagtgg ccgacgtgct cgtcgtcaac aagtcggacc gtccgggcgc ggatgcgctg
     2041 gccgccgact tgacggcggt catcggattg cgcacgagta cgaaagaccc ctgggcgccg
     2101 ccggtcgtgc agacctcggc caccgaaggc aggggcatcg acgcgttgga cgcggcactc
     2161 gcgggacacc gcgcgcacct cgatgagaag ggccgccggg ccgcgcgccg ccgcgaggcc
     2221 ctgcgcgaac gcgtccgcct gttggtcgaa gagcgcgtgc gcgaggacct gtggcgagac
     2281 cctacccggg ccgcgcggtt tgatggtatc ttggaggcgg tcgtcggcgg gcgtacgtcg
     2341 ccgtaccagg ccgcgcgcga actggctcta ccatgaaccg accgatcaaa gtcctgatcg
     2401 ccaagcccgg cctggacggc cacgaccgcg gcgccaaggt gatcgccaac gcgttgcgcg
     2461 acgccggcat ggaggtcgtg tacacggggc tccaccagac gcccgagatg gtggtcgacg
     2521 ccgcgctgca agaagacgtc gacctggtgt cggtcagcat tctctcgggc gcgcacatga
     2581 cgctcctgcc gcgcgtgcgc gagctcatga acacgcgcgg gctcgcggac gtgaagctga
     2641 tcgcgggggg catcatcccc gacgcggacg ccgaagctct aaagaagctc ccgggtatcg
     2701 ccggcatctt cggccccggg accagcacgc aagacgtcgt caaattcatt caatcgctat
     2761 tcacccatcg aaagtagccg catgtcgcaa gtcctcttga acgaagaaca ccagatgatc
     2821 cgggagaccg cgcgccgctt cgcggaagcg gagctcgtgc cgaacgcggc gcacatcgac
     2881 gcaaccggcg agtacccggt cgagaatttc aagaagatgg gcgagctcgg cttcttgggg
     2941 ttgatcgtcc cggccgagta cggcgggacc ttcatcgaca ccgtctcgta cgcaatcgtg
     3001 atcgaagaac tcgcgcgcgc gtgcggctcg acggccctcg gtgtggcggc gcacaattcg
     3061 ctcgcgtgcg gcccgctgct cttgatgggc agcgaggcgc agaagaagcg cttcctcccc
     3121 gagctcgcga gcggggaggc gatcggcgcc ttctgtttga ccgagccgca ggccggcagc
     3181 gacgcgtcca acaccaaaac gaccgcggtg ttgaagggcg acacgtacat cttgaacggc
     3241 accaagatct acgtcaccaa cggcggctgg gcgcgctacc tgatcgcgac cgccgtcacc
     3301 gaccccggca agggctccaa gggcatcagc gcgttcgtgg tcgagaacga cttcccgggc
     3361 ttcgtcgttg gatccaggga aaagaagctg ggcttgcgcg cgtcggggac ttacgagatc
     3421 gtgttcgaca actgcgaggt tcccaaggcc aacctgctcg gctccgaggg cggcggattt
     3481 cgcaccttca tgaagacgct cgaagccggc cgcatctcga tcggtgcgtt ctcactcggc
     3541 atcgcgcagg ccgcgatgga ggccgcgatc aagttcggca aggatcgcaa gcagttcgac
     3601 acgcccatca ccgagttcca ggccacgcag ctgaagatcg ccgacatggc caccgagatc
     3661 cacgccgcac gcctgttgat ccacgacgcc gcgcgccgca aggacgcggg cgaagatttt
     3721 gggcgttacg cgtcgatggc caagttgttc gcgtccgaga tggccatgcg cgtcaccgac
     3781 caggccattc aaattcacgg cggttacggg tactgcaagg agtaccacgt cgagcgcttc
     3841 tatcgcgacg ccaagctggg cgagatcggc gaaggcacga gcgaaatcca acgcatcatc
     3901 atcgcgcgcg cggttctcgg cgacgaataa cccgcgcatc gaaggagaaa ttaccgacat
     3961 ggaacgagcc gtcatcgtat ccgcgcttcg cacccccatc gggcgcttca tgggcgggtt
     4021 gtcgaccctg cccgcgccgc gtctggccgc ggggctcatc aaggagaccc tcgcgcgcac
     4081 caagctcgac tcggagacca tcgacgaggt gatcctcggt aacgtgctgc aagcgggtgt
     4141 ggggcaagcg cccgcgcgcc aggccatgat cttcggcggt gtggcgccgt cggtgggtgc
     4201 ggtcaccatc aacaaggtgt gcggctcggg attgaagtcg gtcatgctgg cggcacaggc
     4261 catcaaggcc ggcgacgccg aggtcgtgct cgcgggcggc atggagagca tgagcatggc
     4321 gccgtacctc ctgcccgacg cgcgcgcggg gcagcgtctg ggccacggcc gcttgatcga
     4381 cgccatgatc aacgatggcc tgtgggactg ctacaacgac taccacatgg gcaacaccgg
     4441 cgaattggtg gcgaaaaagt acggcatatc gcgcgagacc caggacgagt tcgcggtcga
     4501 gagtcacaag aaggccgtcg ccgcggcgga gcgaggcgac ttcaaagacg agatcgtgcc
     4561 cgtcatggtg ccgcagccca agggcgaagc gctcgcggtc gccgtcgacg aagggccgcg
     4621 cgccgatgcc tccaaggaga agctcgccaa gctcaagccc gcattcgaaa aggacggcac
     4681 cgtcaccgcc ggcaacgcga gcaccatcaa cgacggcgcc tcggtcctgc tggtcatgac
     4741 cgagaagaag gccaaggagc tcggcctcga ggtgctcgcg gtcatcgacg cctacgcgac
     4801 cggcggcatg gagccgcagt gggtgatgat ggcgcccgtc aaggcggtcg ggaagctgct
     4861 ggagcgtacc aagacgcgca tcaccgacta cgacgtcatc gagctgaacg aggccttcgc
     4921 ggtgcagggc gtggcgctca ttcgcgagct caagattccg cccgagagac tcaacgtcaa
     4981 cggcggtgcc gtggccctcg gccaccccat cggcgcctcc ggcgcgcgtg tattgacgac
     5041 cctcattcac gccatgcgca agcgacgcgc caagcgcggg ctggtgtcgc tgtgcctggg
     5101 cgggggcaac gcggtcgcga tgagcgtgtc gctgccgcaa tgaa
//
LOCUS       DASPGK010000350         6053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1725693, whole genome shotgun sequence.
ACCESSION   DASPGK010000350 DASPGK010000000
VERSION     DASPGK010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725693"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..548)
                     /locus_tag="VEC56_09800"
     tRNA            complement(473..548)
                     /locus_tag="VEC56_09800"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(513..515),aa:Thr,seq:cgt)
     gene            complement(670..2301)
                     /locus_tag="VEC56_09805"
     CDS             complement(670..2301)
                     /locus_tag="VEC56_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14486.1"
                     /translation="MSVVHRLYHLARADFFERVRRHGFLITMLFCVYAGYAFLPPNPS
                     PYATLKLDQYRGVYNSAWVGTAVAMLSAVFISMIGFYVVKNAIQRDRHTRVGQILATT
                     PISRVQYTLGKTISNFAVLAAMALVVAIACIGMQVLRAEDRSIDLVRLLAPFVLLTLP
                     MLFVTAALAVLFETLPLMRGGFGNVAYFFLFGAFLAAGAGNESRVSSNEVTGMGIAIP
                     SMIEAAHAAYPEFEPDSVSVSMGINIRSEGEWSLKTFEWNGIDWTLQSIGWRMSWVLV
                     GLVVGAAAAIPFDRFDPARGRGDSIRAASRRWGRRRRATAREVVDDAEVEATPSHANV
                     HLTPLPAGARGIRLGAMVMAEWKLVVRGLRWWYAGPIAALIASIFAPLSGVQAIVLPL
                     AWFWPVLQWSKLGTREARFGTGAIFFSTPHPLARQLFATWIAGVMLAVLAAAPVAVRF
                     AIAGESASLAAWCVGAAFIPALALALGVWTGSGKFFEVLYTALCYAVVQHVPPMDFMG
                     AIPEATEKGYPTVYAGVTLVLLAIALLGRRRQVRG"
     gene            complement(2298..3182)
                     /locus_tag="VEC56_09810"
     CDS             complement(2298..3182)
                     /locus_tag="VEC56_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV14487.1"
                     /translation="MKLAIQNVGKTYRGGVRGLDAFSLQLGPGVLGLLGPNGAGKSTL
                     MRILATITRPSEGRVEWNGVDIVAKPDALRAELGYLPQDFGIYPNLNAQEFLEYIAAA
                     KGIAARAARTRIDELLALVNLSDARRRPLGGFSGGMRQRVGIAQALLNDPKLLIVDEP
                     TAGLDPEERVRFRNLLSDLSGERIVILSTHIVSDVEATATHIALINKGRLVVSSSPED
                     LLRTVEGRVWEWMVSSAELTAAKQRYRISSTIRRSDGVRVRAVSETAPSAGAQRVAPS
                     LEDAYLFHIAPAAAGTPA"
     gene            3479..4126
                     /locus_tag="VEC56_09815"
     CDS             3479..4126
                     /locus_tag="VEC56_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HXV14488.1"
                     /translation="MVPALWMLFATVVQAGEIRGTIRIVSDVAAPMSAGLDPYAGTLN
                     SVGTSDARGPRSSTPKDVVVYLEGPGTRDAARDTARPELWQINQSFEPHVLGVPVGAT
                     IDFPNGDLVYHNVFSYSKPKKFDLGYYGKGKSKSVTFDKPGIVQVFCDIHSTMSAYVL
                     VVDTKFVTQPDENGEYVLPDVPGGNYTLKVWHPDLGERSVKAAVGDGVTRLDVSI"
     gene            4161..>6053
                     /locus_tag="VEC56_09820"
     CDS             4161..>6053
                     /locus_tag="VEC56_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV14489.1"
                     /translation="MLRFGSLRARIVVLTTAPLIMILVAVLLSTMQTANDTVRRRVNE
                     SLAEAGSVFVQMVTSRRNELVSMAQVTVRDPRFFAPFSIPREERGAEFVPTLEGIAAD
                     FLRITDADFMEMFDADGRFITRVSRGGSDDRLARGATSPAYGSSPGAAGLDLAMRGSA
                     IADFYEYDGRIVVAAVAPVYVLHNLEAVIRLGSFLTDEFAGEVKRLTGADVCLARNGA
                     AFASTYAAKPGQTIDWSPRGAVATTIARGSVTLSEAFSREHDGREYLTIHVGVGGVAP
                     EDGFDALIGRELMSEMTPILMLEKRLALGGLFAVVVTLVVGFLVANSITAPLSRIVAA
                     SVALQKGQYTYPLDLRGKDEIALLGRNFALMRESLATYVQKLKNLDQAKSNFIALAGH
                     ELRTPLTIITGFNEMIASGAMGQLPDKVKETTTMITEQLTGLNKLVQSMLDLTYYEQG
                     LQSLQLARRDVREIVREAAVTRRAIAESRSLRLTLALGEEPLHAVLDPRRLTDAFLAL
                     LDNAIRFTADGGTVHVTARQIESEVHVTVEDTGIGIPQHELKWIFEKIYEVGDVMHHS
                     SGAYGFGSKGFGLGLALCKAIVERHGGRITVSSTPGRGSTFAIVLPRSGPAAPATTPA
                     PHKEEGV"
BASE COUNT         1086 a         2034 c         1982 g          951 t
ORIGIN      
        1 tgtggcggct ggtgaacgac gcctggcagc aaggcccgcc ggtcccggga acgcgcgcgg
       61 atcgccaacc cggcaaggcg accggctggg ccggatgagt gtacgataat tggtgctgct
      121 cagatagtcc gacgaaagcg cttcgcgcgc ctcgtcgtga atccggcagt cgacaccgtt
      181 gcacagaacg acgcggtcgc cgctcggcgg tgcggcgtcg acgacgccat acacgcatac
      241 aaggaaggta agcatgcacg tcgcgcgttg caccgcgctt tctcgctcgg ttccgacaat
      301 ttgcgttacg gtccttcgac atggctcacc cccgacgggt cgcacgagcg actggtgaga
      361 gacccgcttc agtcctgctc acggcagaat cataccagga aacaccgcaa gatcgccgaa
      421 ggggagggat ccagtctcgc tgatcgaacg gcgtgacgaa ggggaggaaa actggagctg
      481 gtgcgcggaa tcgaaccgcg gacctgcgca ttacgaatgc gctgctctac cggctgagct
      541 acaccagctc cgaaaggcgt ttcgagatcg cgtcgggatc aatccctgcg gatcgcggaa
      601 ggcctcgttt ataccacact cggccccagc ccgccagtgc ttccgaaacg gggtggcgac
      661 gccgcggact tagccccgca cctggcgccg acggcccaac aacgcgatcg cgagcagcac
      721 gagcgtcacg cccgcgtaaa cggtcgggta tcccttctcg gttgcctcgg ggatggcgcc
      781 catgaagtcc atgggaggga cgtgctggac cacggcgtag cagagcgcgg tgtacagcac
      841 ctcgaagaac ttccccgatc cggtccacac gcccagtgcc agcgccaatg ccggtatgaa
      901 cgcagcgccc acgcaccacg cggcgagtga cgccgactct cccgcgatcg cgaagcgcac
      961 ggcgacgggt gccgcggcca gcacggccag catgacaccg gcgatccagg tggcgaacaa
     1021 ttggcgcgcc agcgggtgcg gcgtcgagaa gaagatcgcg ccggtcccga agcgcgcctc
     1081 acgcgtgccg agcttcgacc attggaggac gggccagaac cacgcgagcg gcaatacgat
     1141 cgcttgtaca cccgagaggg gggcgaagat ggacgcgatc aacgccgcga tcggtcccgc
     1201 gtaccaccat cgcaagccgc gcacgacgag cttccactcg gccatcacca tcgcgcccag
     1261 acgaatgcct cgcgcgccgg cgggaagcgg cgtcaggtgg acgttggcgt gcgacggagt
     1321 cgcttccacc tcggcgtcgt cgacgacctc gcgcgcggtc gcacgccgac gacgtcccca
     1381 ccggcgcgac gccgctctta tcgagtcgcc cctgccgcgc gcgggatcga agcggtcgaa
     1441 cggtatcgcg gccgcggctc ccacgacgag accgaccaac acccacgaca ttcgccagcc
     1501 aatactctga agagtccagt cgattccgtt ccactcgaac gtcttcaggc tccactcccc
     1561 ctccgagcga atgttgattc ccatcgacac cgagacgctg tcgggctcga actcgggata
     1621 cgcggcgtgg gcggcttcga tcatgctggg gatcgcgatg cccatccccg tcacctcgtt
     1681 gctcgagacc cgcgattcgt tgccggcacc ggcggctaga aacgccccga acaagaagaa
     1741 gtatgcgacg ttcccgaagc cgccgcgcat gagcggcaac gtctcgaaca acaccgcgag
     1801 tgccgcagtg acgaacagca tggggagcgt cagcagcacg aagggcgcga gcaagcgtac
     1861 caagtcgatc gagcggtcct ccgcgcgcag gacctgcatg ccgatgcacg cgatcgccac
     1921 caccagcgcc atcgcggcca acaccgcgaa gttgctgatg gtcttgccga gcgtgtactg
     1981 aacgcgcgag atgggtgtgg tggcgaggat ttgccccacg cgcgtgtgcc ggtcgcgctg
     2041 gatcgcgttc ttgaccacat agaatccgat catcgagatg aacaccgcgg agagcatcgc
     2101 gaccgcggtg cccacccagg ccgagttgta cacgccgcga tactggtcca gcttgagggt
     2161 ggcgtagggg ctggggttgg gtggcaggaa ggcgtagccc gcgtacacgc agaacagcat
     2221 cgtgatcaga aatccgtggc ggcgcacgcg ctcgaagaaa tcggcgcgcg cgaggtgata
     2281 cagccgatgg acgacgctca cgcgggtgtg ccagccgcgg cgggcgcgat gtggaacagg
     2341 taggcgtctt cgaggctggg cgctacgcgc tgcgcgcccg cgctgggtgc cgtctccgaa
     2401 accgcgcgca cgcgcacgcc gtcgctgcgc cgaatcgtgg acgagatgcg gtagcgctgc
     2461 ttggccgcgg tcaactcggc gctggacacc atccactccc acacgcgccc ttccacggta
     2521 cgcagcagat cttcgggcga ggacgacacg accagcctgc ccttgttgat gagcgcgatg
     2581 tgggtggcgg tggcttcgac gtcggagacg atgtgcgtcg acaagatcac gatgcgctcg
     2641 cccgagagat ccgagaggag gttgcgaaag cgcacgcgct cttcggggtc gaggccggcg
     2701 gtgggctcgt cgacgatcag gagcttggga tcgttcaaca aggcttgcgc gatgccgacg
     2761 cgctggcgca tgccgccgga gaatccgccg agcgggcgcc gccgcgcgtc cgagagattc
     2821 acgagcgcca agagctcgtc gatgcgcgtg cgcgccgcgc gcgcggcgat tcccttcgcg
     2881 gccgcgatgt attccaaaaa ctcctgcgcg ttcaagttgg gatagatccc gaagtcctgc
     2941 gggaggtacc cgagctcggc gcgcagcgcg tccggcttgg cgacgatgtc gaccccgttc
     3001 cattcgacgc gaccctcgga tgggcgcgtg atggtggcga ggatgcgcat cagggtggac
     3061 ttgcccgccc cgttggggcc caggagtccc agcacaccgg gacccaactg gagtgaaaac
     3121 gcgtcgagcc cgcgcacacc accacggtag gtcttgccga cattctgaat cgctagcttc
     3181 atgaacggtt tggaagcgtc ggattgtagg gccgacacgg cagcgcgctc aacctcccaa
     3241 ttcctgcccc ctccttgtac ggcacgaccg ttgcacgggg cttccaggga ttcccgtatc
     3301 gtgcggctgg ctcccaggag cagcttgtca aaccgacggt tggcgcgcgt cgcaccccct
     3361 cgcaaaaagg gcctggctcg cgtcgtaacc cattggaagt gattctgtta tgactcattc
     3421 cacgcttcga ctccgacgcc cggccaagcg ccgaggccga ccccgctggt ggatcgtttt
     3481 ggttcctgcc ctgtggatgc tttttgccac cgtcgtgcag gccggcgaga tccgcgggac
     3541 catccggatc gtctcggacg tggccgcccc catgtccgcg gggctggacc cctacgccgg
     3601 caccctgaat tcggtcggca ccagcgatgc gcgcggccct cgctcgagca cgcccaagga
     3661 cgtcgtcgtg tatctcgaag ggccgggcac gcgcgatgcc gcgcgcgaca ccgcgcgccc
     3721 cgagctgtgg cagatcaacc agtcgttcga gccccacgtg ctcggcgttc ccgtcggcgc
     3781 caccatcgac ttccccaacg gcgacctcgt ttatcacaac gtgttctcgt actcgaagcc
     3841 gaagaagttc gacttgggtt actacggcaa gggcaagtcg aagagcgtca ccttcgacaa
     3901 gccggggatc gtgcaagtgt tctgcgacat ccactcgacc atgtcggcat atgtgctcgt
     3961 ggtcgacacg aagttcgtca cgcagccgga cgaaaacggc gagtacgtgc tgcccgacgt
     4021 tcccggcggc aactacacgc tgaaggtctg gcacccggat ctgggcgagc ggtcggtcaa
     4081 ggcggccgtc ggcgacggtg tgacgcgtct cgacgtgagc atttgatccg cgcgtaccac
     4141 tactagcaag acggtaagcc atgctgcgat tcggatcatt acgagcacgc atcgtcgtcc
     4201 tgacgacggc gccgctgatc atgatcctgg tcgccgtgct gctctcgacc atgcagacgg
     4261 ccaacgacac cgtgcgccgc cgcgtcaacg agagtctcgc cgaggcgggc tcggtcttcg
     4321 tgcagatggt gacgtcgcgg cgcaacgaac tggtcagcat ggcgcaagtg acggtccgtg
     4381 atccgcgctt cttcgcgccc ttctcgatcc cgcgcgagga gcgcggcgcc gagttcgtgc
     4441 ctactctgga aggcatcgcg gcggacttcc tgcgcatcac cgacgccgat ttcatggaga
     4501 tgttcgatgc cgacggccgc ttcattacgc gcgtcagccg cggcggaagc gacgaccgcc
     4561 tcgcgcgcgg tgccacctca cccgcgtatg gatcgagccc gggcgcggcg ggccttgacc
     4621 tggccatgcg cggctccgca atcgccgact tctacgagta cgacggacgc atcgtggtgg
     4681 ccgcggtggc gcccgtgtac gtgctccaca acctggaggc ggtcattcgc ctcggcagct
     4741 tcttgaccga cgagttcgcg ggcgaagtca agcgcctgac cggcgctgac gtctgcctcg
     4801 cgcgcaacgg cgccgccttc gcgagcacct acgcggcgaa gccgggccag accatcgact
     4861 ggagcccgcg gggcgcggtc gcgaccacca tcgcgcgcgg atcggtgacg ctctcggagg
     4921 cgttcagccg cgagcacgac gggcgcgaat acctgacgat tcacgtcggc gtgggcgggg
     4981 tcgcgccgga agacggcttc gacgcgctga tcggccgcga gctcatgtcc gagatgaccc
     5041 ccatcctgat gctcgagaag cgcctggcgc tgggcggctt gttcgcggtc gtggtgacgc
     5101 tggtggtggg cttcctggtc gcgaatagca tcaccgcgcc gctctcccgc atcgtggccg
     5161 cgtcggtggc gctccaaaag ggccagtaca cctacccgct ggacttgcgc ggcaaggacg
     5221 agatcgcgct cctgggcagg aattttgcgc tcatgcgcga gtcgctcgcc acctacgtgc
     5281 aaaagctaaa gaacctcgac caggccaaga gcaacttcat cgcgctcgcc ggccacgaac
     5341 tgcgcacccc gctcaccatc atcaccggct tcaacgaaat gattgcgagc ggtgcgatgg
     5401 gccagctgcc cgacaaggtc aaggaaacca cgaccatgat caccgagcag ctgaccggct
     5461 tgaacaagct cgtgcagtcg atgttggacc tgacctacta cgagcagggc ctgcagtcgc
     5521 tccaactcgc gcggcgcgac gtgcgcgaga tcgtgcgcga agcggccgtc acgcggcgcg
     5581 cgatcgccga gagccggagt ctgcgcctga cgctcgcgct gggcgaggag ccactccacg
     5641 ccgtcctcga tccgcgccgg ttgaccgacg ccttcctggc gctcctcgac aacgcgatcc
     5701 gtttcaccgc cgacggcggc acggtgcacg tcaccgcgcg ccagatcgag agcgaggtcc
     5761 acgtcaccgt cgaggacacc ggcatcggca tcccgcagca cgagctcaag tggattttcg
     5821 agaagatcta cgaagtgggc gacgtcatgc accactcgtc gggcgcgtac gggttcggtt
     5881 ccaaagggtt cggtctgggt ctggcgctgt gcaaggccat cgtcgagagg cacggcgggc
     5941 ggatcacggt ttccagcacg cccgggcgcg gcagcacgtt cgcaatcgtc ctgccccgtt
     6001 ccggtcccgc ggcgcctgcc accacgccgg caccgcacaa ggaagaagga gtc
//
LOCUS       DASPGK010000351         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1021467, whole genome shotgun sequence.
ACCESSION   DASPGK010000351 DASPGK010000000
VERSION     DASPGK010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021467"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VEC56_09825"
     CDS             complement(<1..223)
                     /locus_tag="VEC56_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14490.1"
                     /translation="MKSRPERILLAMIALQLLHAVEEFVFEFWNAFPPMRAVYGDVSG
                     VGVFVLFHSLLILFGVWCYRELRRGGITSY"
     gene            complement(220..1197)
                     /gene="selD"
                     /locus_tag="VEC56_09830"
                     /pseudo
     CDS             complement(220..1197)
                     /gene="selD"
                     /locus_tag="VEC56_09830"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            1490..2044
                     /locus_tag="VEC56_09835"
     CDS             1490..2044
                     /locus_tag="VEC56_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14491.1"
                     /translation="MKIVKAGFLGAIVLLAHASAGLAAPVVYDEGVDGDLDQSNPPVF
                     VFDVGVNSWTGTIGPTPTSNTQDSFFANLPTDMTIVHIHWEYSVNNQDISYFDVSGPV
                     EAAPPFGTVLSHKVSRFGDNLTDTGFSLVNPILPITVVGQYKCEVTTGFAVATSTWRI
                     EITVERNVLPTEPSTWGQIKSLYR"
     gene            complement(2170..>2508)
                     /locus_tag="VEC56_09840"
     CDS             complement(2170..>2508)
                     /locus_tag="VEC56_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14492.1"
                     /translation="SWGAAMGPVVLALEPRLKVGVLVVAGLLFQPSQPEADPMAYIRH
                     VQAPVLMLNGKYDFFFPYETSQVPFYELLGTPRDHKRLVVHEVGHAIPAADRARESLA
                     WLDRYLGPVR"
BASE COUNT          468 a          831 c          770 g          439 t
ORIGIN      
        1 cgtatgacgt gatgccgccg cgccgcagct cccggtagca ccagacaccg aagagaatca
       61 gcaacgagtg gaagagcacg aagacgccca cgccggaaac gtcgccgtag accgcgcgca
      121 tgggcgggaa ggcattccag aactcgaaga cgaactcctc gaccgcgtgc aggagctgca
      181 gcgcgatcat ggcgagcagg atccgctcgg ggcgggactt cattcgacga cgatggagac
      241 gtcgcgccgc gcgacgcact cgcccaccac ggcaacgcgc gggtggttgg tcttgagatc
      301 gtcgagcagt cgtggcgcgt tctcgcgcgc caccgcgatc aagagccccc ccgacgtttg
      361 cggatcgaac atgagcgcgg tcgtggtctc gtcgaccgag ggcgccaggc gcgccttgtc
      421 ccgcatgaag cgttgtgtgg ccttgccgcc gcccgtgaga tggccctgct ccaccgcctc
      481 gcgcgcgccc tcgagcacgg ggacgttcgc ggagcgcacg acgatcgtca cgccgcttcc
      541 ttccgccatc tcgttggcgt ggccgacgag cccgtagccc gtgatgtcgg tgcacgcgtg
      601 cgggtcatgg cgaagcatcg cctccgacgc gctccggttg agcaacgtca tcgagtcgac
      661 cagcgcgcgc gtccgctccg gagagagcgc gcgctgcttc aaggcggtcg cgagcacgcc
      721 ggtcccgatg ggcttggtaa ggatcaagac gtcgccggcg cgcgcgcccg cgttgcgcac
      781 gatgcggcgc gggtcgatca atccgactac agcgagcccg tacttcacct cggcgtcgtt
      841 gacggtgtgg ccgcccagga tcgacacgcc ggccgcgcgc gcgacatcgg acccgccgcg
      901 aatgatctcg cccagcacgt cggccggaat cttgttctcg ggaaagcccg cgatgttgag
      961 ggccacgacg ggccgccccc ccagtgcgta cacgtccgag agcgagttgg tggccgcgac
     1021 gcggccgtag tcgtacgcgt cgtcgacgat gggagtaaag aaatccaccg tgactaccat
     1081 ggccgcatcg tccgagagac gatacacccc ggcgtcgtcg cttccggcgt gacccaccat
     1141 tacgttgggg tcggtcggaa cgggcaactt cgcgagtatg tcgcgcagcc ccgccgcgct
     1201 gaatttagag gctcaaccgg cgcatgccgc gaactcggtc aggcgaaaca aacgatcctt
     1261 ttcggacatg ggattgtcac ctcctccggc tgcgtttccg gccgatctcg tcggatggtc
     1321 gagaacgcgc gtcccgcata cagacgcatt cttgcatccc gtccgccatg caacaagtgc
     1381 ggtgtcgatt ttttccgccg cgacgacgaa cgggagtaca atagcatgcc cgggccgaaa
     1441 acccggctaa ccacaatgtg gccgcaggaa acacaaagag gttcgagcca tgaaaatcgt
     1501 taaagctgga ttcttgggcg cgatcgtatt gctggcccat gccagcgcgg gcctggcagc
     1561 gccggtagtc tatgacgaag gcgtcgatgg cgatctggac caatccaacc cgcccgtgtt
     1621 tgttttcgat gtcggcgtca actcgtggac cggcaccatc ggaccaacgc ctacctcgaa
     1681 cacgcaggac tcatttttcg ccaacctccc gacggatatg accatcgtgc acatccactg
     1741 ggaatattcg gtcaacaacc aggatatctc gtacttcgac gtctccggac cggtcgaggc
     1801 ggctccgccg ttcggcacgg tcctgtccca caaggtttcg cgcttcggcg acaacctcac
     1861 ggacaccggg ttcagtttgg tcaatccgat tctcccgatc acggtcgtgg ggcagtacaa
     1921 gtgtgaggtg accacggggt tcgcggtcgc aacttcgacc tggaggattg agatcaccgt
     1981 cgagagaaac gtacttccca ccgagccgtc cacgtggggc cagatcaagt cgctttatcg
     2041 ctagtctcga tatacaagga ccggccaccc gcgaggttcg ggtggccgtc tttccttctt
     2101 ccgccgtcga tgacatactc cggtgtctct agttcgactt cttcgcagcc cccaccaatc
     2161 ccgcacgagt tatcgcaccg gccccaagta ccgatccagc cacgcgagtg actcccgcgc
     2221 gcgatccgcc gcgggaatcg cgtgcccgac ctcgtgcacc acgagcctct tgtgatcccg
     2281 cggcgttccg aggagttcgt agaatgggac ttgcgacgtt tcgtaaggaa agaagaaatc
     2341 atatttgccg ttgagcatca agacgggcgc ttgcacgtgt cgaatgtacg ccatcgggtc
     2401 ggcttccggc tgtgacggtt gaaacaggag tcccgccacc accagcacgc ccactttgag
     2461 ccgcggctcg agcgcaagca cgaccggtcc catcgcggcg ccccaact
//
LOCUS       DASPGK010000352         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_554670, whole genome shotgun sequence.
ACCESSION   DASPGK010000352 DASPGK010000000
VERSION     DASPGK010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554670"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VEC56_09845"
     CDS             complement(<1..973)
                     /locus_tag="VEC56_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXV14493.1"
                     /translation="MVPILIPLLLMASPADDWSTEFERTGGRLTSTYAQTIEYCRRLD
                     KASKWIRFESFGKSGEGRDLPLLIVDKNGDFSAERAHKRGKAIVFVQAGIHSGEIDGK
                     DAGLILVREMAITRERADLLDRITLVFIPIYNVDGHEHVQPYNRVNQKGPENAGFRAT
                     ATFLNLNRDYMKADAPETRAWLSLWNRWDPDFFVDCHVTNGADYAYVVTYLMEVFANA
                     DPGVAAWSRDRFVPELEPRMRESGFPVVHYVEFRRDHDPASGLKTWASTPRFSTGYVA
                     LANRPALLVETHMLKDYATRVTGTHAILQRTLEIVGEQADEVRRINVE"
     gene            1086..>2171
                     /locus_tag="VEC56_09850"
     CDS             1086..>2171
                     /locus_tag="VEC56_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV14494.1"
                     /translation="MRIALIALLVAAFALPASARTWRIRVDGTGDLPTIQAAVNACAP
                     GDSILVAPGTYTWTNQNTLPNDLYAMIEFNEGRRDITLVSEAGPQATILDAQWQGRVL
                     VTNGLNYAIVDGFTLTGGYAPGLSDALGGAMILHISHDTFKNCIFRDNFATFGGVAWV
                     GGLAQPSFIDCEFMNNTATYGGALLLVNTSTPITIRRCRFHHNTAESGGGAIYMNHVL
                     AYVDDSVFAFNQAPQGGAIFMRSMWAATIARCTFVRNMGSDAGVLYILASPAIRVESS
                     IVAYGGDGTPYFVSANSAVTFACNDTYRNPVSNDLPPTAIDGGGNFTADPLFCGSVDS
                     MNYTLGLASPCLPGNHPAGASCGQVGAY"
BASE COUNT          377 a          733 c          686 g          375 t
ORIGIN      
        1 cctccacgtt gatgcggcgc acctcgtccg cctgctcgcc gacgatctcc agcgtgcgct
       61 gcaggatcgc gtgcgtcccg gtgacgcgcg tcgcgtagtc tttgagcatg tgggtttcga
      121 ccaacaacgc cggccggttt gcgagtgcga cgtaaccggt ggagaagcgc ggcgtcgacg
      181 cccacgtctt cagtccgctc gcgggatcgt ggtcgcggcg aaactcgacg tagtgcacga
      241 ccggaaagcc cgactcgcgc atgcgcggct cgagctcggg gacgaagcga tcgcgcgacc
      301 acgcggccac acccgggtcc gcgttcgcga acacctccat gaggtaggtc accacgtagg
      361 cgtagtcggc gccgttggtg acgtggcaat cgacgaagaa gtcggggtcc catcgattcc
      421 acagcgaaag ccaggcccgc gtctcgggcg cgtcggcttt catgtagtcg cggttgaggt
      481 tcaggaaggt cgcggtggcg cgaaagcccg cgttctcggg acctttctgg ttgacgcgat
      541 tgtacggttg cacgtgctcg tgcccgtcca cgttgtagat ggggataaag accagcgtga
      601 tgcgatcgag caaatccgcg cgctcgcgcg tgatcgccat ctcgcgcacg agtatcagcc
      661 ccgcgtcttt gccgtcgatc tctcccgagt ggatgcccgc ctgcacaaac acaattgcct
      721 taccccgctt gtgcgcgcgc tcggcggaga agtcgccgtt cttgtcgacg atcaacagcg
      781 ggagatcgcg cccctcgccg ctcttgccga acgactcgaa gcggatccac ttcgacgcct
      841 tgtcgaggcg ccggcagtac tcgatggtct gggcatacgt ggacgtgaga cggccgcccg
      901 tgcgctcgaa ctcggtcgac cagtcgtcgg cgggggacgc catcagcaat agaggaatca
      961 ggatcgggac catggcgggc cagtatacga aggggaaccc cgtcgtgcaa ttctgccgtt
     1021 gcgcgggacg gacgcgggta ttaacgttgg agctacccca cgcaatcgtc ttgcgtatgc
     1081 accccttgag aatcgcactg atcgccctgc tcgtggccgc gttcgcgctc ccggcgagcg
     1141 cccgaacctg gcgcatccgc gtcgacggca ccggggacct gcccaccatc caagccgcgg
     1201 tcaacgcctg cgcaccgggg gactcgatcc tggtcgcacc cgggacctac acctggacca
     1261 accagaacac gctccccaac gacctgtacg cgatgatcga gttcaacgag ggacgccgtg
     1321 acatcacgct ggtgagcgaa gcgggccccc aggcgaccat cctcgacgca cagtggcagg
     1381 gccgggtact ggtcaccaac ggactcaatt acgcgatcgt ggacggattc accctgaccg
     1441 gcggctatgc gcccgggttg agcgacgccc tgggcggcgc catgatcctt cacatttcgc
     1501 acgacacctt caagaactgc atctttcgcg acaacttcgc gaccttcggc ggcgtggcgt
     1561 gggtaggggg cctcgcgcaa ccgagcttca tcgactgcga gttcatgaac aacaccgcga
     1621 cctacggggg tgcgctgctc ctggtcaata cgtcgacgcc catcaccatc cgacgctgcc
     1681 gattccacca caacacggcc gagagcgggg gcggggcgat ctacatgaac cacgtcctgg
     1741 cgtatgtcga cgactcggtg ttcgcgttca accaggcccc gcagggtggc gcgatcttca
     1801 tgcgcagcat gtgggcggcg actatcgcgc gctgcacgtt cgtgcgcaac atgggttccg
     1861 acgccggcgt gctttacatc ttggccagcc cggcgattcg ggtcgagagt tcgatcgtcg
     1921 cctacggagg ggacgggacc ccgtacttcg tctccgccaa cagcgcggtg accttcgcct
     1981 gcaacgacac ctaccgcaac ccggtgtcga acgacctgcc gccgaccgca atcgacgggg
     2041 gcggcaactt caccgcggac cccctgtttt gcgggtcggt cgactcgatg aactacaccc
     2101 tcggtctcgc ctcaccgtgc ctgcccggca atcaccccgc gggagcgtcc tgcgggcaag
     2161 tcggcgccta c
//
LOCUS       DASPGK010000353         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_671704, whole genome shotgun sequence.
ACCESSION   DASPGK010000353 DASPGK010000000
VERSION     DASPGK010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671704"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1523
                     /locus_tag="VEC56_09855"
     CDS             309..1523
                     /locus_tag="VEC56_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14495.1"
                     /translation="MRARSITTIRNGWVVAASLLAACASAPAQPGPDADLNAIKRRAS
                     LQIGAGDYAEAVRSLQSYLKSQPKDDQAYAMLGDAYRGLGDFANAVKSYEQAMRIDYG
                     DYQPHLKLGTLLMENGKTGRALTEFEVAMKFSDGDPLVYYNYGLALHDLGRTKEALPQ
                     WRAALELEPDNPDFVAAVGIGLTGVDDQAAVEAFHKAETLGQRRDGDFCNNYALALER
                     VGENARADTLFNEAVAIGGPKQNEYRRNLARHYLRAGDNESAAREFETLVASDGGKWS
                     DTVYLARARVALARFDEAIDGLSSLAAEVESGRVARTDPRIDRMPPTLGEALSIVGMA
                     WRGNGDLARSRDYLRRAAVQAPDDPSVLNNYGVVLAESGMLPDAKAQWRRVLEIDPAN
                     ATAKANLSAFGQ"
     gene            1593..2012
                     /locus_tag="VEC56_09860"
     CDS             1593..2012
                     /locus_tag="VEC56_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14496.1"
                     /translation="MYCKYCGRGVDEKSELCPHCGRRIRSSVSAALEGSEWTRLRPLR
                     EPKSPGLAGFLGFVLSWIVMGPVGYIYLGQWNWFFIAFVVTLIAYPLTFGVAYALFPF
                     VFAFHQYQMARELNETLARKPDVATPGTEAGSPGPLA"
     gene            2032..2988
                     /gene="glpX"
                     /locus_tag="VEC56_09865"
     CDS             2032..2988
                     /gene="glpX"
                     /locus_tag="VEC56_09865"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HXV14497.1"
                     /translation="MERNLALELVRVTEAAALASARWMGKGDGEAADRAARDAVEVVM
                     RSIKMNGRVVIGDAENAPAAPARKAHAKTGAVPDVDVAVDALQSIDSVAFGRPYAIAT
                     IAVASPDSFLVPPVPYMNKIAVGPEAAGCVDIKASPLDNLVNIAAAKRCYVEDLTVCI
                     LDRDRHRALIDEVRGAGARIELISDGDLTAMLATAIASSGIDVVMGVGGSAEGVLAAA
                     ALKCVGGDIQAQFHPRDEKEAEALRAAGFARKDRVLRADDLVRGENAMFAATGVTNSE
                     VLRGVHFVPGGAVTHSIVMRSKSGTVRRITANHRFDRKPVYK"
     gene            3023..>4100
                     /locus_tag="VEC56_09870"
     CDS             3023..>4100
                     /locus_tag="VEC56_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXV14498.1"
                     /translation="MSEPFNLLPPDPAPGRPAPLADKMRARTLDEFVGQESIVGPGTL
                     LRATLEAGEITQSMILWGPPGCGKTTLARLIASRVKNRYVTFSAVTSGIAEVKRVIED
                     ARKLRAVEKRPLLLFVDEIHRFNRAQQDAFLPHIEDGTIVFIGATTENPSFEVIGPLL
                     SRSRVFVLNALTREHVVSILERALSDRERGLGEAQITADDGVLELIADIANGDARVAL
                     NALEYAGSALARSAKPRTLSRAIAMDALQQRVVATDKAGESHYNLISALHKSLRGGDA
                     DAGLYWLARMCEAGEDPLYIARRLVRFASEDVGNADPQALVVAIAARDAVDFIGYPEC
                     KLALAQCVVYLALAPKSNAIYTAYG"
BASE COUNT          666 a         1402 c         1377 g          655 t
ORIGIN      
        1 gcgcctggaa gctcgatcgc aagcacccgg cgccggcctc ggagctctct cgtatgggca
       61 ttcgtcgccc gcccgtgttt tcgttcctgc cccgcaagca ctggctcaac atacacgcgg
      121 gccgtctgcg cgcccgcgtg ctacgtacca aggctcgcgt cgtttcctga taaactagcg
      181 ctgcgcggcg ggtcctgtcg cctcgccacc ccgcgggcat gtcgcccgcg gggccccgtc
      241 tcggtgccac ccgccgcggt tcgccccgtg ctcgcattgc atccgtccgc gcggtatatt
      301 caccccgcat gcgcgcgcgt tctatcacta cgattcgcaa cggttgggtt gtggccgcga
      361 gtttgctcgc ggcgtgcgcg tccgcgccgg cgcagccggg gcccgacgcc gacctcaacg
      421 cgatcaagcg gcgcgcttcg ctccagatcg gggcggggga ttacgccgag gcggtgcgct
      481 ctctgcagtc ctacctcaag tcgcaaccca aggacgacca ggcgtacgcg atgctgggcg
      541 acgcctatcg cggtctcggc gactttgcca acgcggtcaa gagctacgag caggcgatgc
      601 gcatcgacta cggcgactac cagccgcact tgaagctggg gacactgctg atggagaacg
      661 gcaagaccgg tcgcgcgctg accgagttcg aagtggcgat gaagttctcc gacggcgatc
      721 cgctggtgta ctacaactac gggctcgcac tgcacgacct gggtcgcacg aaggaagcgc
      781 tcccgcagtg gcgcgccgcg ctcgagctgg agcccgacaa ccccgacttc gtcgccgccg
      841 tcggcatcgg gttgaccggc gtcgacgacc aagccgcggt ggaggcgttc cacaaggcgg
      901 agacgctcgg ccagcgacgc gacggcgact tctgcaacaa ctacgcgctg gcactcgagc
      961 gcgtgggcga gaacgcgcgc gcggacacgc tcttcaacga ggcggtcgcg atcggcggcc
     1021 ccaagcagaa cgaataccgt cgcaacctcg cacgacacta tttgcgcgcc ggggacaacg
     1081 agagtgccgc gcgcgagttc gagaccctgg tcgcgagcga cgggggaaag tggagcgaca
     1141 cggtgtacct cgcgcgcgcc cgggtggcgc tggcgcgctt cgacgaagcg atcgacggac
     1201 tgtcgagcct ggccgccgag gtggagtcgg gccgggtcgc gcgcaccgac ccgcgcatcg
     1261 atcgcatgcc gccgaccctg ggcgaagcgc tctcgatcgt cggcatggcg tggcgcggca
     1321 acggcgacct cgcgcgctcg cgcgactacc tgcgccgcgc cgccgtccag gcccctgacg
     1381 acccctccgt gctcaataac tatggcgtcg ttctcgcgga aagtggtatg ctgccggacg
     1441 ccaaggccca gtggcggcgg gtgctggaga tcgaccccgc gaacgccacc gcgaaggcca
     1501 atctttccgc attcggacag taacaatcga tgtttgcccg ccgtagaacc gcatgggtgc
     1561 ccgacgtttc ttgatccggt aaggtggtaa ccatgtactg caaatattgc ggccgcggag
     1621 tcgacgagaa atcggagttg tgcccgcact gcgggagacg cattcgcagc tcggtttcgg
     1681 cggcgctcga gggcagcgag tggacccggc ttcggcccct gcgtgagccc aagagccccg
     1741 ggctggccgg ttttctgggc ttcgtgctgt cgtggatcgt gatgggtccc gtgggctaca
     1801 tctacctcgg ccagtggaat tggttcttca ttgccttcgt cgtcacgttg atcgcctatc
     1861 cgctgacgtt cggtgtggcc tacgcgctgt tcccgttcgt gttcgcgttc caccagtacc
     1921 agatggcgcg cgagctgaac gagaccctgg cgcgcaagcc cgacgtcgcc actccgggga
     1981 cggaagcggg ttcgcccggc cccctcgcct gaccgcgaaa cgccccagcc gatggaacgc
     2041 aacctcgccc tcgagctggt ccgcgtgacc gaagccgccg cactcgcgtc ggcgcgctgg
     2101 atgggcaagg gcgacggcga agccgcggat cgcgcggcgc gcgacgcggt cgaggtggtg
     2161 atgcgctcga tcaagatgaa cgggcgcgtc gtcatcggcg acgccgagaa cgcccccgcg
     2221 gcgccggcgc gcaaggcgca cgccaagacg ggcgctgttc ccgacgtcga cgtcgccgtc
     2281 gacgccctgc aatccatcga ctcggtcgcc ttcggtcgtc cctacgccat cgccaccatc
     2341 gccgtcgcga gccccgattc gtttctggtg ccgcccgtcc cgtatatgaa caagatcgcg
     2401 gtgggccccg aagccgccgg ctgcgtcgac atcaaggcca gcccgctcga caatctggtc
     2461 aacatcgcgg ccgccaagcg ctgctacgtc gaggacctga cggtgtgcat cctggatcgc
     2521 gaccgccacc gcgcgttgat cgacgaagtg cggggcgcgg gtgcccgcat cgagctgatc
     2581 tccgacggcg atttgaccgc gatgctcgcc accgccatcg cgtccagcgg gatcgacgtc
     2641 gtgatgggtg tgggcggcag cgcggagggc gtgctggcgg cggcagcgct caagtgcgtg
     2701 ggcggcgaca tacaggcgca gttccacccg cgcgacgaaa aggaagcgga ggcgttgcgc
     2761 gcggccgggt tcgcgcgcaa ggaccgcgtg ctgcgcgccg acgacctcgt gcgcggcgag
     2821 aacgcgatgt tcgccgccac cggggtgacc aacagcgagg tcctgcgggg cgtgcacttc
     2881 gtgcccgggg gggcggtgac gcactcgatc gtgatgcgca gcaagtcggg gaccgtgcgc
     2941 cgcatcaccg ccaatcatcg tttcgaccgc aagcccgttt ataagtaatc gtggcgacgc
     3001 gatagcgtcg ctaccgggtg acatgagcga acccttcaat cttctcccac ccgaccccgc
     3061 ccccggaagg ccagctccgc tggccgacaa gatgcgcgcg cgcacgctgg acgagttcgt
     3121 ggggcaggag agcatcgtgg gaccggggac actcctgcgc gcgacgctgg aggccggcga
     3181 aatcacgcag tcgatgatcc tgtggggccc gcccgggtgc ggcaagacca ccctcgcgcg
     3241 cctgatcgcg tcgcgcgtca agaaccgcta cgtcaccttc agcgccgtca cctccggaat
     3301 cgccgaggtc aagcgcgtca tcgaggacgc gcgcaagctg cgcgcggtcg aaaagcggcc
     3361 gctgttgctg ttcgtcgacg agatccaccg cttcaatcgg gcccagcagg acgcgtttct
     3421 cccccacatc gaagacggca ccatcgtgtt catcggcgcc accaccgaga acccgagctt
     3481 cgaggtgatc gggcccttgt tgtcgcgctc gcgcgtgttc gtgctgaatg ctttaacccg
     3541 cgagcacgtc gtgtcgattc tcgagcgcgc gctttcggat cgcgagcggg gcctggggga
     3601 ggcacagatt acagccgacg acggagttct ggaactgatt gccgacatcg ccaacggcga
     3661 tgcgcgggtg gccctgaatg ccctggaata cgcgggttcc gctctcgcga ggtcggccaa
     3721 accacgcacg ctatcgcgtg cgattgccat ggacgcgctg cagcaacgcg tggtcgccac
     3781 cgacaaggcc ggcgagtcgc actataatct aatctcggcg ctccacaaga gcctgcgcgg
     3841 cggcgacgca gacgcgggac tgtactggct cgcgcgcatg tgcgaagccg gagaggaccc
     3901 gctctacatc gcgcggcgat tggtacgctt cgcctccgag gatgtcggca atgccgaccc
     3961 gcaggcgttg gtggtcgcga tcgccgcgcg cgacgcggtc gacttcatcg gctacccgga
     4021 gtgcaagctg gcactggcgc agtgcgtcgt ttatcttgca ctcgctccca agagcaacgc
     4081 gatctacacc gcctacggtg
//
LOCUS       DASPGK010000354         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1726356, whole genome shotgun sequence.
ACCESSION   DASPGK010000354 DASPGK010000000
VERSION     DASPGK010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726356"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VEC56_09875"
     CDS             <1..580
                     /locus_tag="VEC56_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14499.1"
                     /translation="EDPFGLSRASGRRDANAQRFVGDGLCLSDVLLVREEAPARGPGP
                     DGAIERGGRRMRPNVERQYADGERLRTYLEIYSLALATDGGARTSSYDLRYAIFPART
                     ESDPAWVDWGRRAAEWAGFGEDDEAVISQTFRREGRSHDDRETIAIDIDVLDDGRYEL
                     LVEVTDRRSGQRAVVHAPFWKETGPVADGKKR"
     gene            complement(581..940)
                     /locus_tag="VEC56_09880"
     CDS             complement(581..940)
                     /locus_tag="VEC56_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14500.1"
                     /translation="MRSISLRNKHFAQIVVLAVCGTMVLAPLPRASNDPPVTVAANAH
                     FLRDAVPAAGFAHAPEDTTEDDPFLSEEKDTSQLVWEIAAWVVGAALVAFFIIKVFIE
                     EDPDETDEGGSTKPDPF"
     gene            complement(1107..1721)
                     /locus_tag="VEC56_09885"
     CDS             complement(1107..1721)
                     /locus_tag="VEC56_09885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXV14501.1"
                     /translation="MASTPTRLTIPNLVTASRLVMAVLAAWLAARADEPEAAVVVCVV
                     AALLDAFDGWYARAFAQCSRVGEHMDPMADKVLMGVVYGWVGIDAGSAIVWSLVALVG
                     VREAAMTVFRSYSLRRHGRYIPASRFGRTKMLVQSVVGLGILGATHLAGIAVPVPVVV
                     GGLVIILVLSYASAVAYAVDWKNRGSLREEPLREAAARRAVANS"
     gene            1850..>2260
                     /locus_tag="VEC56_09890"
     CDS             1850..>2260
                     /locus_tag="VEC56_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14502.1"
                     /translation="MTSAERSDARRRWVIAAAASLLLHASLLLWWRGGGLGPGTGPAG
                     GSEGGSGGAAGVAPGSIIEVSIDVDAPVPAGATIPADEVAIETSTLPAESTVPQPPAT
                     ESSAPIPTDAPPDASGEGSGRGDKTGAGEGTGEGS"
BASE COUNT          380 a          783 c          790 g          307 t
ORIGIN      
        1 cgaggacccc ttcggcctct cgcgcgcttc cgggcgtcgg gacgcgaacg cgcaacgctt
       61 cgtgggcgac gggttgtgcc tgagcgacgt gctcttggtg cgcgaggaag cccccgcgcg
      121 cggcccgggc ccagacggag cgatcgaacg cggcgggcgc cgcatgcgtc ccaacgtcga
      181 gagacagtac gcggacgggg aacggctgcg cacgtacctc gagatctaca gcctcgcgct
      241 cgccaccgac ggcggagcgc gcacctcgtc gtacgacctc cggtacgcga tcttccccgc
      301 gcgcacggaa tcggatccgg cctgggtcga ctgggggcgg cgcgcggccg agtgggcggg
      361 ctttggcgag gacgacgaag cggtcatctc gcaaaccttc cgccgcgagg gacgctcgca
      421 cgacgatcgc gagaccatcg cgatcgacat cgacgtgctc gacgacggcc gctatgagtt
      481 gctggtcgaa gtgacggacc gcaggtcggg gcagcgcgcg gtggtacacg cgcccttctg
      541 gaaggaaacc ggcccggtgg ccgacgggaa gaagcgctga tcagaacgga tcgggtttgg
      601 tgctgccgcc ttcgtcggtc tcgtcggggt cttcctcgat gaagaccttg ataatgaaga
      661 acgcgaccag cgcggccccc acgacccacg ccgcgatctc ccaaaccagc tggctggtgt
      721 ctttctcttc cgagagaaac gggtcgtctt cggtcgtgtc ttcgggcgcg tgcgcgaagc
      781 cggccgcggg cactgcgtcg cggaggaaat gcgcgttcgc ggccacggta accggcgggt
      841 cgttggacgc gcgcggcaac ggtgccagca ccatcgtgcc gcacaccgcg agcacgacaa
      901 tctgggcaaa gtgcttgtta cgaagtgaaa tcgatcgcac cgcgaagaag atagcgcccg
      961 gccgccacct tgacaatggc ggaggcgtcc cctaccttca acgtgctacc agcctaacca
     1021 tggtgatgcg tagcgcaaag tgggtctgct cctggtcggc ggcaggcccc tttgctttgg
     1081 gttcgatccg gccgccccgg gacgcactac gaattggcca cggcgcggcg cgcggcggcc
     1141 tcgcgcaggg gctcctcgcg gaggctcccc cgattcttcc agtcgacggc ataggcgacc
     1201 gccgaggcgt acgagagcac gagaatgatg acgaggccgc ccaccaccac cggcaccgga
     1261 accgcgatcc ccgcgagatg ggtcgccccc aggatgccga gtcccacgac actttgcacg
     1321 agcatcttgg tgcgcccgaa ccggctcgcg ggaatgtagc gaccatgccg gcgaaggctg
     1381 taggatcgaa ataccgtcat cgcggcttcg cgaaccccga ccagtgcgac cagcgaccag
     1441 acgatcgccg agcccgcgtc gatcccgacc cagccgtaca ccacacccat cagaaccttg
     1501 tcggccatcg gatccatgtg ctcgccgacc cgcgagcact gcgcgaacgc gcgcgcgtac
     1561 cagccgtcga acgcatccag cagtgcggcc acgacgcaca ccacgaccgc ggcctcgggc
     1621 tcgtccgcgc gcgcagcgag ccacgcggcc aggacggcca tcaccaggcg cgaggcggtg
     1681 acgagattgg gaatcgtcaa gcgtgtgggc gtggacgcca tggtcgacct tcggacgccg
     1741 tcgcgcccgg gctcggggat gggcgcgggc aaacacggcc ttgcgtctcg accatatcac
     1801 acagccgtca cccccgccat gcgggcgcgc ttcgaggggc cggatggcga tgacgagcgc
     1861 tgagcgcagc gatgcacgtc gacgctgggt catcgcggcc gccgcgtcgc tcctgcttca
     1921 cgcctcgctc ttgctgtggt ggcgcggggg cgggctggga ccgggcacgg gtcccgcggg
     1981 cggaagcgag ggcggaagcg gcggtgccgc cggcgttgcg ccggggtcga tcatcgaggt
     2041 ctcgatcgac gtggacgctc ccgttcccgc cggcgcgacc attcccgccg acgaagtcgc
     2101 gatcgagaca tcgacgctgc cggccgaatc gaccgtgccc caaccaccgg cgacggagtc
     2161 gtccgcgccc attccgaccg acgcgccacc ggacgcgagc ggcgagggga gcgggcgcgg
     2221 cgacaagaca ggcgcgggcg aaggcaccgg cgaaggctcg
//
LOCUS       DASPGK010000355         5014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_86986, whole genome shotgun sequence.
ACCESSION   DASPGK010000355 DASPGK010000000
VERSION     DASPGK010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86986"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VEC56_09895"
     CDS             <1..340
                     /locus_tag="VEC56_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14503.1"
                     /translation="ETGLVHETTSVYVPVRSASLGQNYPNPFNPTTRIEYRLPEAGPG
                     GKTDVSLVVYDVRGARVRVLVSGEQAVGKHVVEWDGRNDAGSPVGSGVYFYRMTTPGF
                     ADVRKMVLLK"
     gene            671..>5014
                     /locus_tag="VEC56_09900"
     CDS             671..>5014
                     /locus_tag="VEC56_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXV14504.1"
                     /translation="MFSRRSARVCSVLCFASLVIACIIGAPLAAAASDLVFTVTAPER
                     PKVVASAGGDRILVDGAGWDAVREPGLPELPYRVVRVLLPQGTTAGDLTVSASEPRRI
                     ATRVRPILAEPFVTSEGGVAQPAASYSGEGVFPDARARLLGVGFLHGFAIASVAVYPF
                     ELRGGDLYVFDRIDVAVATRAGDIAPVVRERYRAGFLEDMRARLEGVVVNPIAIDSYS
                     MGNVVVPKPRGGFQPTAFPSLEGSAVDYVIVTTDALAASFQPLADWKTDKGVPTVIRT
                     VEWILANARNGSDTPETIRNFVIEAYAKWGVTFVLVGGDTEVIPTRFAFSAFYDDGKE
                     LPVDMYLGGLDGDWNADHDPVFGEFGVDAADLYSEVYVGRLPARTPAEVGVLINKVMD
                     YERPVFRTYGKKILLLGEVLFPADYDPGDPISLNGADLLDYVRQSWMTDPALEITPMY
                     ETPAMYPGPPVAIPLSRQAAIDSLNAGQNHVVHIGHGFRFNMSLGDASLVNGDAAALV
                     NGGRLCNLNMLNCTAVAYTYDCLAEHFLRNPNGGAVSTVGANDSAFPNASTYYIEEFY
                     RLLFNQGVTSLGELFARSREPRTPLALASDGVDLWTHYVYTLLGDPEMPLWSDAVKVL
                     AVNHVASVNKGTNNITVTVTESALPVEGARVCLTKDEDDYEFGTTDINGQVTLSFRAE
                     SAGTIKVVVTGPNFKRYEGNITVGGTGAYLRVSSVAIDDDNVNGTVGNNNDIVEAGET
                     VDFALTIQNTGTATTSGTLSVRFRSSTPGVTVIDSLANIGGSIAAGNSVVATSGPRVV
                     FASSMTDEAVAAFVAHIRVNSVHTWRDEFKREVRSPALSLVNLRIDDTGTGNGNGVVE
                     AGEQFRLFYRVKDFGTGAYPGGNATLTAVDPFFTITDGTDPYPAFASQSEAENPAGFI
                     LTETDVATEHRLRLSIVDSFGRAYVDTFELRPPTPPANVVVDPSLGSDRLKITFDASP
                     SPDARFYNVYRSNSGGGPFTKVNVDPVSHTLYVNTGLSATTNYFYRATTVDASGNESA
                     PSALQSGSTNPAQLVGWPIQMELETTSSPAVGDIDGDGDLEIVVGDRYVYAWHDDGFE
                     LVDGDQSALTWGILNTQGQSFVSHVALATVDQVPGLDIIAASRDTKQVFVFNSAGAVL
                     PGWPRPVENFIRAGIAVGDLDGYFGNEIIAIDEMGVVYAWNADGSEYVDGDANPATQG
                     VLKRLPGAVTFHYPTPALADIDGDGKDEIVVGTMADQLHALNDNGSNVPGFPVAFGGD
                     VCGSPAIGDIDNNGDLEIVVNIASGSVVALHHTGATMWTRFIPNGLFFAPSPALADVI
                     GDAKLETFIPSSNGKLYGLSDIGTDLAGFPATYSTTTYSESSPVIADIDGDGLRDIVL
                     GGEEKYIWGWNRNGAVLAGFPLSTGDAMRGVPTVTDVDQDGSVDLVAAGWDKTVYVWD
                     FVGPWNPTNAPW"
BASE COUNT          857 a         1643 c         1668 g          846 t
ORIGIN      
        1 cgagacgggg ctggtgcacg agacgacgag cgtgtacgtg ccggtacgaa gtgccagcct
       61 ggggcagaat tatcccaatc cgttcaaccc gacgacccgg atcgagtacc ggttgccgga
      121 ggcggggccg ggcgggaaga cggacgtgag tctggtggtc tacgacgttc ggggggcgcg
      181 ggtgcgggtg ctggtgagcg gcgagcaggc ggtgggcaag cacgtggtgg agtgggacgg
      241 gcgcaacgac gcgggctcgc cggtgggatc gggagtctat ttctatcgca tgacgactcc
      301 gggcttcgcg gacgtccgca agatggtgtt attgaagtga cgacgaacca gggccgttcg
      361 ggtcgcatga ccctcctcga cacatcagcg gtcccggacg gcccctggtt tccaactccc
      421 cgttgaccgc ggggaggcgc ttggtttatg attggggcca cttcaaggga ggcccgcttc
      481 gggcctcgat gagccttggg ctgggcccca aacgattcgg gtacagacaa ccgctggagc
      541 cacgcacgac cgaactgtta tcgacgcgag tcgttcatcc aaatcgatag tgtgccgaat
      601 cgtgattgtc ccgcgggccg gtcgatgtcg tgaccggtcg aggggcgccg cagccgtgga
      661 gggtcatcgc atgttttcgc gacgcagcgc acgcgtctgt tccgtcctct gtttcgcttc
      721 tctcgttatc gcatgcatca tcggcgctcc gctggccgcg gccgcgtccg acctcgtctt
      781 cacggtgacc gcgccggagc ggcccaaggt ggtggcgtcg gccggcggcg accgtatcct
      841 ggtggacggc gcgggctggg atgcggtgcg cgagcccggt ctacccgagc tcccgtatcg
      901 cgtcgttcgc gtactgctgc cgcaaggcac caccgcgggc gacctcaccg tctccgcgtc
      961 ggagccgcgg cgcatcgcca cgcgcgtccg cccgatcctg gccgaaccgt tcgtcaccag
     1021 cgaaggcggc gtggcgcaac ccgcggcctc ttattccggc gaaggcgttt tccccgacgc
     1081 gcgtgcgcgc ctgttgggtg tcggcttcct ccacggattc gcgatcgcga gcgtcgccgt
     1141 atacccgttc gagctgcgcg gcggggacct ctacgtgttc gatcgcatcg atgtcgccgt
     1201 ggccacgcgc gcgggagaca tcgcgcccgt cgtgcgcgag cgataccgcg ccggattcct
     1261 ggaagacatg cgcgcgcgcc tcgaaggcgt ggtcgtcaac ccgattgcga tcgacagcta
     1321 ttccatgggc aacgtggtgg tacccaagcc gcgcggcgga ttccagccca cggcgtttcc
     1381 ctcgctcgag ggcagcgcgg tcgactacgt gatcgtcacc accgatgcgc tggccgcgtc
     1441 gttccagccg ctcgccgact ggaagaccga caagggtgtt ccgaccgtga ttcggaccgt
     1501 cgagtggatc ctcgccaacg cacgcaacgg gtcggacact cccgagacga ttcgaaactt
     1561 cgtcatcgaa gcgtacgcga agtggggcgt caccttcgtg ctggtcggtg gcgacaccga
     1621 ggtgattccc acgcgcttcg cattcagcgc gttctacgac gacggaaaag aactcccggt
     1681 cgacatgtat ctgggcgggc tcgacggcga ctggaacgcg gatcacgacc ccgtcttcgg
     1741 ggagttcggc gtcgacgctg cggacctcta ttccgaagtg tacgtggggc ggctgcccgc
     1801 gcgcaccccg gccgaggtcg gcgtgctgat caacaaggtg atggactacg agcgtcccgt
     1861 gttccgaacc tacggcaaga agatattgct cctcggcgaa gtgctgttcc cggccgacta
     1921 cgacccgggc gatcccattt cgctcaacgg ggccgacctg ctcgactacg tgcgccagtc
     1981 ctggatgacc gaccccgcgc tcgagatcac gccgatgtac gagacgccgg cgatgtatcc
     2041 gggtccgccg gtcgcgattc cgttgtcccg gcaggcggcc atcgactcgc tcaacgcggg
     2101 gcagaaccac gtcgtccaca tcggccacgg attccgtttc aacatgtccc tgggcgacgc
     2161 gagcctggtc aacggcgacg cggccgcgct cgtcaacggc ggccggctgt gcaacctgaa
     2221 catgctcaac tgtaccgccg tcgcctacac ctacgattgc ctggccgagc acttcctgcg
     2281 caaccccaac gggggcgcgg tctcgaccgt gggagcgaac gacagtgcgt ttccgaacgc
     2341 gtccacgtac tacatcgagg agttttatcg cctgctcttc aaccagggcg tgaccagtct
     2401 gggcgagctc ttcgcgcgct cgcgcgaacc gcgcacgccg ctggcgctgg cgagcgacgg
     2461 cgtcgacctg tggacccact acgtctacac gctgctcggt gaccccgaga tgccgctgtg
     2521 gagcgatgcg gtcaaggtgc tggcggtcaa ccacgtcgcc agcgtcaaca agggcaccaa
     2581 taacatcacg gtcaccgtga ccgaaagcgc gcttcccgtc gaaggcgccc gggtgtgcct
     2641 gaccaaggac gaggacgact acgagttcgg cacgaccgat atcaacggcc aggtcaccct
     2701 ctcgttccgc gccgagagtg cgggcacgat caaggtggtc gtcacggggc cgaacttcaa
     2761 gcgctacgag ggaaatatca cggtgggcgg gacgggcgcg tacttgcgcg tatccagcgt
     2821 tgccatcgac gacgacaacg tcaacggtac cgtcggcaac aacaacgaca tcgtcgaggc
     2881 gggcgagacg gtcgatttcg cgctcacgat ccagaacacc ggtaccgcta ccacgagcgg
     2941 gaccttgagc gtgcgcttcc gttcctcgac gccgggggtc acggtgatcg acagcctcgc
     3001 gaacatcggc gggtcgatcg cggccggcaa cagcgtggtc gcgaccagcg gtccgcgcgt
     3061 cgtcttcgcc agcagcatga ccgacgaagc ggtcgccgcc ttcgtcgccc acatccgcgt
     3121 caacagcgtg cacacgtggc gcgacgagtt caagcgggaa gtccgcagcc cggcgctctc
     3181 gctcgtcaac ctccgcatcg acgataccgg cacggggaac ggcaacggcg tggtggaagc
     3241 gggcgagcag ttccggttgt tctaccgcgt gaaggacttc gggacgggcg cgtaccccgg
     3301 cggcaacgcg accctgaccg cggtcgatcc gttcttcacc attaccgacg gcaccgaccc
     3361 gtaccccgcc ttcgcgagcc aatcggaagc ggagaatccg gccggtttca tcctaaccga
     3421 gaccgatgtc gccaccgagc atcggctaag gctatcgatc gtggattcgt ttggacgcgc
     3481 ctacgtcgac acgttcgagt tgcgaccgcc gacaccgccg gccaacgtcg tggtcgatcc
     3541 cagcttgggc tccgaccgcc tcaagatcac cttcgacgcc agccccagcc ccgacgcccg
     3601 gttttataac gtctaccgat ccaatagcgg cggcggaccg ttcacgaagg tcaacgtcga
     3661 ccccgtctcg cacaccctct acgtcaacac cgggctgagt gccacgacga actacttcta
     3721 tcgggcgact acggtggatg catccggcaa cgagagcgct ccatcggcgt tgcaatcggg
     3781 gagcaccaac cccgcgcagc tcgtgggttg gcccatccag atggagctcg agaccacgtc
     3841 gtcgccggcg gtgggggaca ttgacggcga cggtgatttg gagatcgtgg tgggcgaccg
     3901 ctacgtgtac gcgtggcacg acgacggctt cgagctcgtg gacggcgacc agagcgcgct
     3961 gacatggggt attttgaata cgcaggggca gagtttcgtg tcgcacgtgg cactcgcgac
     4021 cgtcgaccag gtacccggcc tcgatatcat cgcggcctcg cgcgacacca agcaggtgtt
     4081 cgtgttcaac tccgcgggag cggtgttgcc cgggtggccg cggccggtgg agaacttcat
     4141 ccgggccggt atcgcggtcg gcgacctaga cggctacttc ggaaacgaga tcatcgccat
     4201 cgacgagatg ggtgtggtgt acgcctggaa cgccgacggg agcgagtacg tcgacgggga
     4261 cgcgaacccg gccacccagg gcgtgctcaa acgcctgccc ggcgcggtga cgttccacta
     4321 tcccacgccc gcgctggccg acatcgacgg cgacggcaag gacgagatcg tggtcggcac
     4381 catggccgat cagctgcacg cgctcaacga caacgggtcg aacgtgcccg gcttcccggt
     4441 tgcgttcggc ggcgacgtgt gcggcagccc cgccatcggc gacatcgaca acaacggtga
     4501 cctcgagatc gtggtcaata tcgccagcgg gagtgtcgtg gcgctgcacc acaccggagc
     4561 cacgatgtgg acgcgattca ttcccaacgg gctcttcttc gcgccctcgc ccgcgctggc
     4621 cgacgtcatc ggcgatgcca agctcgagac gttcatcccc agctcgaacg ggaagctcta
     4681 cgggctttcg gatatcggta ccgacctcgc cggttttccg gccacgtact cgacgaccac
     4741 gtattccgag tcttcgcccg tgatcgcgga catcgacggc gacggcctgc gcgacatcgt
     4801 gctcggcggg gaagagaaat acatttgggg ctggaatcga aacggcgccg tgttggcggg
     4861 cttcccgctc tcgaccggcg acgcgatgcg cggggttccg acggtgacgg acgtggacca
     4921 ggacggctcg gtggatttgg tggcggcggg ctgggacaag acggtgtacg tgtgggactt
     4981 cgtggggccg tggaacccga cgaacgcgcc gtgg
//
LOCUS       DASPGK010000356         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_87047, whole genome shotgun sequence.
ACCESSION   DASPGK010000356 DASPGK010000000
VERSION     DASPGK010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87047"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VEC56_09905"
     CDS             complement(<1..1323)
                     /locus_tag="VEC56_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV14505.1"
                     /translation="MRGIIAFAVRRRVTVVMTSLAVIAFGIVGYNRLPLELFPDISYP
                     SLTVQTDFPDTAPQEVENLVTRPVEEAVGVLRGLKSIHSVSRSGVSEVTLEFDWDSDM
                     DMLSMEVREKLDRLILPTEAEEPIVLRFDPSLDPIMRIALSGPGELNEMRNLADRKIK
                     LDLETIKGVASAQIKGGLEDEIHIDVHQERLAALGIPIDDVRRVIGVSNVNLPGGSLR
                     GADMQYLIRTLNEFDTVDEIGEVIVAERDGAAVRLRDVATVRMGAKEREEITRVDGHE
                     CIEIAAFKEGDANTVTVARLLRDRIDYWNGKLPEGHKLSLLFDQSHFIEQSIKEVRNS
                     AVIGGLLAVVVLFFFLREFRSTAIIATAIPLSVVATFLLMYQLDISLNVMSLGGLTLG
                     IGMLVDNSIVVLESIFRKRKQGVPLARAAVEGTDEVAAAVTASTLTSVA"
     gene            complement(1333..2484)
                     /locus_tag="VEC56_09910"
     CDS             complement(1333..2484)
                     /locus_tag="VEC56_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV14506.1"
                     /translation="MSSTPRFRRTWIAPALVLAVAAALGACNKGGGAGQAQGAGAPGG
                     APGNGAPKQPPVPVAIAAASTGSIASYYTATATLAADKEAELMARVSGVVERLLCEEG
                     DVVAEGQELLLIDNDEYAFRLEQAVADRKDLETRMERMQTMRDQDMVSAEEYETVVND
                     LSAAKAAEGMARLNLSYTRVTAPFAGRVVTRNVDPGQTVNVGTQLFVLSDFTPLLARV
                     HVPSKEFNKLKPDQPVDLVLDSNGTKLRGRIRLVSPTIDPSSGTIKVTVEINEYPAGV
                     RPGDFAQVSIVTERRNNSTLVPKIAVVNDRGEQIVYVSQADTTAERRVVELGFQDDQN
                     AEILTGVAVGDRVVVKGQRSLKHGAAIKVLDGENAAQAAGDTAAGAGGS"
     gene            complement(2683..>2927)
                     /locus_tag="VEC56_09915"
     CDS             complement(2683..>2927)
                     /locus_tag="VEC56_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14507.1"
                     /translation="GFNLSAEAEDKDHGETFDLDVETFEFGATFGGGIEYTMSSMSII
                     GDFRFALGATSISDEFDGKNSGVGMMVGVKFPLGAR"
BASE COUNT          486 a          966 c          961 g          514 t
ORIGIN      
        1 cgccacactg gtcagagtcg acgccgtcac cgccgccgcg acctcgtcgg tgccttcgac
       61 cgcggctcgc gcgagcggga cgccctgctt gcgcttgcga aagatcgact ccagaaccac
      121 gatcgagttg tcgaccagca tgccgatgcc gagtgtgagc ccgcccagcg acatgacgtt
      181 gagcgagata tcgagttggt acatcaagag gaacgtcgcc accaccgaaa gcggaattgc
      241 ggtcgcgatg atggccgtgc tgcggaactc acgcaggaag aagaagagca ccaccacggc
      301 caggagaccg ccgatgacgg ccgagttgcg tacttccttg atcgattgct cgatgaagtg
      361 cgactggtcg aagagcagcg agagcttgtg gccttccggc agcttcccgt tccagtaatc
      421 gatgcgatcg cgcaggaggc gcgccacggt gaccgtgttg gcgtcgcctt ccttgaaggc
      481 ggcgatttcg atgcactcgt ggccgtcgac gcgcgtgatc tcctcgcgct ccttggcccc
      541 catacgaacc gtggcgacgt cgcgcagccg caccgcggcg ccgtcgcgct cggcgacgat
      601 cacttcgccg atttcgtcca cggtgtcgaa ctcgttgagt gtgcgaatca gatactgcat
      661 gtcggcgccg cgcagcgacc cgcccggcag gttgacgttg gacacgccga tgacgcggcg
      721 cacgtcgtcg atgggaatcc cgagtgcggc caggcgctcc tggtgcacgt cgatgtggat
      781 ctcgtcctcg aggccgccct tgatctgcgc ggaggccaca cccttgatgg tctccaggtc
      841 gagcttgatc ttgcggtcgg ccagattgcg catttcgttg agctcgccgg gcccggagag
      901 tgcgatgcgc atgatggggt cgagcgaagg gtcgaagcga aggacgatcg gttcctccgc
      961 ctcggtagga agaatcaagc ggtcgagctt ctcgcggacc tccatcgaga gcatgtccat
     1021 gtccgagtcc cagtcgaact cgagggtgac ctcggacacg ccggagcgcg acacggaatg
     1081 gatcgacttc agtccgcgca gcacgccgac cgcttcctcg accgggcgcg tgacgaggtt
     1141 ctcgacctcc tggggcgccg tgtcggggaa atcggtctgg accgtgaggg acggatacga
     1201 gatgtcgggg aatagctcga gcgggaggcg attgtaaccg acgataccga acgcgatgac
     1261 agcgagggac gtcatcacga ccgtgacgcg ccgccgaacc gcgaatgcga tgatcccgcg
     1321 catgcggcgc gcctacgatc cgccggcgcc ggctgcggtg tctcccgccg cctgggccgc
     1381 gttctcgccg tccaagacct tgatcgcggc gccgtgcttg agcgagcgct gccccttgac
     1441 caccacgcga tcgccgaccg cgacccccgt cagaatttcc gcgttctgat catcctggaa
     1501 cccgagctcc accacgcggc gttccgcggt cgtgtcggct tgcgagacgt agacgatctg
     1561 ctcgccgcgg tcgttgacga ccgcgatttt cggcaccagc gtcgagttgt tgcgccgctc
     1621 ggtgacgatg ctcacctgcg cgaagtcgcc cgggcgaacg ccggccgggt actcgttgat
     1681 ctcgaccgtc accttgatgg tgccgctcga gggatcgatg gtcgggctca ccagccgaat
     1741 acgtccccgc agcttggtgc cgttgctgtc gagcacgagg tcgacgggct ggtcgggctt
     1801 gagcttgttg aactccttgg acggcacgtg cacgcgcgcc aatagcggcg tgaagtcgga
     1861 gagaacgaag agctgggtgc cgacgttgac ggtctggccg gggtccacgt tgcgcgtcac
     1921 gacgcgcccc gcgaaggggg ccgtcacgcg cgtataggac aggttcaggc gtgccatacc
     1981 ctcggcggcc ttggctgccg acaggtcgtt gaccacggtc tcgtattctt cggccgatac
     2041 catgtcctgg tcgcgcatcg tctgcatgcg ctccatgcgc gtctcgaggt ccttgcgatc
     2101 cgcgaccgcc tgctcgagcc ggaaggcgta ctcgtcgttg tcgatgagga gcagctcttg
     2161 gccctcggcg acgacgtcgc cctcttcgca caggagccgc tcgaccacgc ccgagacccg
     2221 ggccatcaac tcggcctctt tgtcggcggc cagcgtggcg gtcgccgtgt agtagctcgc
     2281 aatactcccc gtcgaggccg cggcgatcgc gacgggaacc gggggctgct tgggcgctcc
     2341 gttgcccggt gcgccgccgg gggctccggc cccctgggcc tgccccgcgc ccccgccctt
     2401 attgcacgcc ccgagggcgg cggcgacggc gaggaccagg gctggcgcaa tccaagtacg
     2461 acgaaaccgt ggggtcgaac tcattctagt aacctacttt gcgtcaggaa gccttacccg
     2521 ccggaccggg gtgaagtggg gataccgggc cgtccatagc cagctcgaac ccgggcagcg
     2581 ggcgcccggc gttgggatgg acaggccata atacgctata gagtgaacgg ggtttcagac
     2641 gatcttcggg gacctcgcgc gggcagtcct cttcggcgcc gcttagcgcg cacccagcgg
     2701 gaacttgacc cccaccatca tgcccacacc gctgttctta ccgtcgaact cgtccgagat
     2761 gctggtggcc cccagcgcga accgaaaatc gccgatgatc gacatcgacg acatcgtgta
     2821 ctcgataccg cccccgaagg tggcgccgaa ctcgaaggtc tcgacatcca ggtcgaaggt
     2881 ctcgccgtgg tccttgtcct ccgcctccgc gctcaaattg aatccga
//
LOCUS       DASPGK010000357        10546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_87187, whole genome shotgun sequence.
ACCESSION   DASPGK010000357 DASPGK010000000
VERSION     DASPGK010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87187"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEC56_09920"
     CDS             complement(<1..459)
                     /locus_tag="VEC56_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14508.1"
                     /translation="MTRFRITSLARTVLLAAGLAAGWGAAPARAQTIVTLFDVRDAVV
                     SPNDEDDQATVRYTLSVVVDTLSLVIFEADSVTAVDTLHTAASEPAGGPKSKFWDGKR
                     WDGSDAAEGAYVVTLFVRKAGNPDFIRSLPVFVDVTPPTVQILSALPSPYA"
     gene            complement(522..1253)
                     /locus_tag="VEC56_09925"
     CDS             complement(522..1253)
                     /locus_tag="VEC56_09925"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HXV14509.1"
                     /translation="MTDALREATAIVRASRRVVVTTGAGMSKESGIPTFRDAPNALWA
                     NFNPEDLATRRGFSRDPALVWRWYAERRAMIASARPHEGHVALARWEERCESFVILTQ
                     NIDGLHTAAGSKNVVELHGNIFRYKCFDNDHAVEGPFPTDHVPPRCACGSLVRPAVVW
                     FGESLDPAHLEAADAALSWCDLLVVVGTSGLVYPAAGFALAAKESGARVIEVNPEQTQ
                     LTEIANVFVAGGARTILPELAGQSG"
     gene            complement(1250..2167)
                     /locus_tag="VEC56_09930"
     CDS             complement(1250..2167)
                     /locus_tag="VEC56_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXV14510.1"
                     /translation="MTASTTRLILAYAAMCVIWGSTWMAIKIGLEGAPPLTSIAVRMV
                     IATAVVFAIVRLWRIPIPRDARFVRLGLFLGLFHIVFPYSLVYYGEQRIPSGQAAVLY
                     ATLPLMVAVLARFVLGDALTARKLFGIATGIAGVAVIFSDSLSGPAGAGGDARAAGVA
                     LVLGSVFASSVGSVATKRWSRGYHPVASLLIPFATGAVVAACGALLFEDASPLRFDTT
                     TWLSIVYLAVAGSVSAFALFFFVIQHLDVTLVSYQTFIIPIIAVLIGVAFGETISPRV
                     GAGAALILAGIALATFWSNPRAGRRPGPP"
     gene            2388..4097
                     /locus_tag="VEC56_09935"
     CDS             2388..4097
                     /locus_tag="VEC56_09935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV14511.1"
                     /translation="MKRISPGLFVVLAAAAAVHLAVALQDFGTLARNGFLYDDSFYAF
                     QIARNIAHGLGATFDGIHATNGFQPLYVAALVPLYWIAGDNPTLPIHLALVLSALLTV
                     ATAFLLHRIVARRASETAALIVAGAWAFSPIVIRQAANGLETALALFCLALSVDYYLA
                     RVRPFAAPSRRAFVTLGALLGMATLARVDLGFLALAMCLDHLLVVRARLRVGDAPVRW
                     RGNVVAAVVACALVWAPWAIYGLVSVGSPISESGRATRYLAIAYAPFFGLASPAGLDD
                     GPDAAFMARHLQRSLKSLKVTPAVHPLLRGTRKLSERVGVGEPVTNALGAIVLSCFFV
                     WWWQRRRGPRAAREFDFLLLFSVMMIAAYSTYVFGVFFFLRYYYPLYFVGMIFAGLAI
                     DDAAAWVRARSIGVRRAALVASGVYAAALLYMGYTSAFRTIRVYGFYDAARWVTTHTD
                     PSETIGVFQGGAIGYLSQRRVVNLDGKVNTDAYQALRAGRLGEYVHEAHIDLVLDSER
                     VLDLFLGPWSKEDRERIAHDTVFVGAEHGVHGWIGYRVTPARGFNAGSSSTPGPTRMQ
                     PGR"
     gene            complement(4209..5069)
                     /locus_tag="VEC56_09940"
     CDS             complement(4209..5069)
                     /locus_tag="VEC56_09940"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXV14512.1"
                     /translation="MVERYARAVRGDTRLLWTQGPGHARDLARGAAADSDIVCLFGGD
                     GTVSEVVNGLMPNPVPMVVVPTGSGNDFASLVDCPRTPEELARVVELGVGYRLDVLDC
                     GDRYCANSIGIGFEAMVTFHSLSIRHLRGLPLYLLAVMKALAEYESNRFTLSFDGDRV
                     VEGEFLLVSIGNGVRAGGGFYLNPGAFPDDGRLDVCTADRMPRARMLALLPSTIHGKH
                     VGKRGVSIHTGTRVRVSAQRPFPLHIDGEYLGRREKPLEVSVTPRCLPVLSRAGGVAR
                     HTHPMERLLR"
     gene            5107..7041
                     /locus_tag="VEC56_09945"
     CDS             5107..7041
                     /locus_tag="VEC56_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14513.1"
                     /translation="MKLMRIIFHGDAGRPRSGAPRLAESCALRKRNRLAILGTALCAA
                     GCPSSSTAAPLTDVWHVGPTPEVRAIRHTGSVLWIGTAAGLYVVDIRNGGILSHVGAG
                     EQLPSVSVRAIAAKGDSVFVGTDEGLSLFRRTDAGGLESSVFTPSSPGRLSAVPLTRI
                     AGLGVGANGDVLLATSGRGAGVITKSGGYSITRRDSLLEDKVFAIVDRADGTRYFATS
                     MGLCAQVNDTSFVSFQAGTGIPRGEVRELVPGERNSFYLRVANQGVFRFDGARAVEVD
                     SPPGVRLRDAASISVGDDGTLWVAGDGWVFARRGGKWARVPVPTEDANARWRVVVADG
                     VGAFAGSSDGRVVALGRGGALRVTLPSGLPAPRVQSLAADGSGGAWFVCDGRLVRADA
                     LSRSVEVENGPRNVRAISVGPDGEVWTAGRWTVRRRTGSTWLDVHPDVIEADPAYTSL
                     YVDEMGAWVGTRSGALYRYDGSVWLRLARAPNTPESIAAIHSADEGAWALTGGRLARS
                     HDGGWRRYPGIDSSAAVVDIARAPNGTWLAATETRMFVFEETKRAWVPAREWSSKTSA
                     GLGPLHGRMRAIAFDGQGRLLVGTTEGVALCAPSGVRWLTAADGLGGGEVADLAVDGD
                     RLWIGFAENGVSVISTRGLW"
     gene            7237..8466
                     /locus_tag="VEC56_09950"
     CDS             7237..8466
                     /locus_tag="VEC56_09950"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV14514.1"
                     /translation="MWQLLFWVSLALLVYIYVGYPVALAALGRLRARARSTPPDSPLP
                     SVCLIISAHDEEAVIREKIENSLRLVYDGLLSVVVASDGSADRTNEIAREYHDHGVEL
                     MHWPARRGKSAVLNEVVRARREDVLVFTDANALFAPDAVARLAERFRTPSVGCVVGEL
                     KYARDASTVGQGESLYWRYESLVKSLESRIESVLVANGAIFAARRSWVRELFLDVAND
                     FQIPFDVAARGAGVVYEPSAVAIERTAELWEEEFGRKVRIVLRGITGYVRLHRRMHAL
                     RAWQFVSHKLLRWSVGVILLVVLASSVALAGSSPWFAAFLGLQLVCYAAAVAAWLVRR
                     RRDVPRVLYVPFYFTMVNAAALVALGRFVAGGRQATWEKAASTRATPPRGLEAGGVGS
                     MPVAMAERGARRRLVEK"
     gene            8479..9663
                     /locus_tag="VEC56_09955"
     CDS             8479..9663
                     /locus_tag="VEC56_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HXV14515.1"
                     /translation="MLFFFQTLIRWRRFIVASAVASAAVLGGVSFLLPEWYTARTSIF
                     PPEPTVAMPMYAELVQQLSAPLLGPIATGAAPETIYIEMVKSRSVGEKVITEFDLMNA
                     YKSERIEDCLEELHSHLGFTLLENGMLIVTFEDRDPERAAAIANRLIELIDETTRGLK
                     VSRAGRTRGFIERQLVDRERLLAQAETDLKEFQEEFNTVDLDEQLRSAMDIVTSLSAR
                     AIALETEMRIMSHYTSKTSEEYIRKQNEYNEVVSQLSKLKSNGDQADRDMVRSYIPAL
                     ADVPDVALKYIRLRRAVEVQTTVYTMLVSEFEKARIEEARDTPVVQVLDTAAPPSLRS
                     RPKRKLFVLVGFLVGVAWSSFVALFNTAWRDSLGKSSVVRQLLEPLAGDLARLRRRAK
                     TP"
     gene            9660..>10546
                     /locus_tag="VEC56_09960"
     CDS             9660..>10546
                     /locus_tag="VEC56_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14516.1"
                     /translation="MISVAARERATLFAIALFPVLAFVALRLFGYMKFARLGAIALAA
                     VAAAAVVFLRPRWGLYALVFYIYSGAGLFLPIQLAAPLISIVLAAVLLDWVRGQVPGL
                     PGAYFWVSVGLFVLAALNSTVVARDMAASLRDIGTFLKVLLVVVLAVHLIRTPDQLRA
                     LCYVVFAGAVGTVALGVLAIAIGLEGAGENQIYGVQIMRFTGAHVDPNKAAAYMCSAL
                     PLGIFAVRHCHSRLTRIAFILAVVVLVVGIFATLSRSVFPSVAVVLLGVILYEIRSRR
                     SFVVLVAILALGVVLAPRY"
BASE COUNT         1566 a         3506 c         3700 g         1774 t
ORIGIN      
        1 agcatacggg ctcggaagcg cgctcaagat ctgcaccgtg ggcggagtca cgtcgacgaa
       61 gacggggagc gaccgaatga aatcggggtt gcccgccttg cgcacgaaga gtgtgacgac
      121 gtacgcgcct tccgccgcgt ccgatccgtc ccatctcttg ccgtcccaga atttgctctt
      181 cgggccgccc gcgggctccg aggcggcggt gtgtagtgta tcgacggcgg tgaccgagtc
      241 ggcttcgaag atgacgagcg agagcgtgtc caccacgacg gaaagcgtgt agcggaccgt
      301 cgcctggtcg tcttcgtcgt tgggcgacac cacggcgtcg cgcacgtcga agagggtcac
      361 gatcgtttgc gcgcgcgcgg gggccgcgcc ccacccggcc gcgaggccgg cggcgaggag
      421 gacggttcgt gcaagcgagg taatgcggaa gcgcgtcaac gtggaccctt ggaattcggg
      481 gggtgtgtcg acaatgagta aatcacgccc cgcggttgcg gtcaaccgct ttggcccgcc
      541 agctcgggca ggatcgtccg cgccccgccg gcgacgaaca cgttggcaat ctccgtcaac
      601 tgggtctgct ccggattgac ctcgatgacg cgcgcgccgc tttccttggc cgccaacgcg
      661 aatcccgccg ccgggtacac gagccccgac gtccccacca ccaccagcag gtcgcaccac
      721 gaaagcgccg cgtcggccgc ctcgaggtgc gcggggtcga gcgactcccc gaaccacacc
      781 accgccgggc gcaccagcga accgcacgcg caccgcgggg ggacgtggtc ggtggggaac
      841 ggcccctcga ccgcatggtc gttatcgaag catttgtagc ggaagatgtt tccgtgcagc
      901 tcgaccacgt tcttcgaccc ggcggcggta tgcaagccgt cgatgttctg ggtcaggatc
      961 acgaacgact cgcagcgttc ttcccagcgc gcgagtgcga cgtgcccctc gtgcgggcgc
     1021 gcggaagcga tcatcgcgcg ccgctcggcg taccaccgcc acaccagcgc ggggtcgcgg
     1081 ctgaatccgc gccgcgtcgc gaggtcttcc ggattgaagt tcgcccacag cgcgttgggc
     1141 gcgtcgcgaa aggtgggaat gccgctctcc ttcgacatgc ctgcgccggt cgtgacgacg
     1201 acgcgccgtg acgcgcgcac gatggcggtg gcttcgcgca gcgcgtccgt catggcgggc
     1261 ccgggcgccg gcccgcgcgc ggattcgacc agaacgtcgc cagcgcgatg ccggccagga
     1321 tcaatgccgc ccccgcgccc acgcgcggcg agatggtctc gccgaaggcg acgccgatca
     1381 gcaccgcgat gatgggaatg atgaaggtct gatacgacac cagggtcacg tccaagtgct
     1441 ggatcacaaa gaagaagagc gcgaaggccg acaccgaacc ggccaccgcc aggtacacga
     1501 tcgacaacca cgtggtggtg tcgaatcgaa gcggactcgc gtcttcgaat agcagcgcgc
     1561 cgcacgccgc caccaccgcg ccggtcgcga agggaatcaa gagcgacgcg accgggtggt
     1621 aaccccggct ccatcgcttg gtggccaccg atcccaccga cgatgcgaac acactgccga
     1681 gcacgagggc cacacccgcc gcgcgcgcgt cgccgcccgc acccgccggt ccgctcaagc
     1741 tgtccgagaa gatgaccgcg acaccggcga tgccggtcgc gatgccgaag agcttgcgcg
     1801 cggtgagggc gtcgcccagg acgaatcgcg ccagcacggc gaccatcaag ggcagcgtcg
     1861 cgtagaggac cgcggcctgt ccggagggga tgcgctgctc gccgtagtac acgagcgaat
     1921 agggaaacac gatgtggaac aacccgagga acaaacccag ccgcacgaag cgcgcatcgc
     1981 gcggaatcgg gatgcgccac aggcgcacga tcgcgaacac caccgcggtc gcgatcacca
     2041 tgcgcacggc gatgctggtc aagggcggcg cgccctccag accgatcttg atcgccatcc
     2101 aggtcgatcc ccagatgacg cacatggcgg cgtacgcgag tatgagtcgg gtggtgctgg
     2161 cggtcacgag aaacgccctc gattcgacgg cggcggtcgg gagaccatag cgcacgccga
     2221 ccggcggggg aagggcgccg gtaacgtgcg ttggggtagc gccttagcct gcttcgcggg
     2281 gtgcgacggt tgacacgccg aggcatcggg ggtagcataa ggaacgataa atcaacggat
     2341 ttacccgaga ggtaacgatt cgaaacgact ggtaacggct cctccccttg aaacgaatca
     2401 gccccggcct cttcgtcgtc ctggcggcgg cggcggcggt gcatttggcc gtcgcgctgc
     2461 aggatttcgg cacgctcgcg cgtaacggtt ttctctacga cgacagcttc tacgcgttcc
     2521 agatcgcgcg caacatcgcg cacggcctgg gtgccacctt cgacggtatt cacgcgacca
     2581 acgggtttca gccgctctac gtcgcggcgc tggtgccgtt atattggatc gcgggcgaca
     2641 acccgaccct gcccatccac ctcgccctcg tcctctcggc cctgctcacg gtcgcgaccg
     2701 cgttcctgct ccaccgcatc gtggcgcgtc gcgcgagcga gaccgccgcg ctgatcgtgg
     2761 cgggcgcgtg ggcgttctct cccatcgtga tccggcaggc cgcgaacggt ctcgagaccg
     2821 cactcgcgtt gttctgcctc gcgctctcgg tcgactatta cctggcgcgc gtgcgcccgt
     2881 tcgcggcccc gtcgcgccgc gcgttcgtca ccctgggagc gctcctggga atggcgaccc
     2941 tggcgcgcgt cgacctcggt ttcctcgccc tcgcgatgtg tctcgatcat ctcctggtcg
     3001 tgcgcgcccg gttgcgcgtc ggcgacgcgc cggtgcgctg gcgcggcaac gtggtggcgg
     3061 ccgtggtggc gtgcgcgctg gtgtgggcgc cgtgggcgat ctacggcctg gtatcggtgg
     3121 ggtcgcccat ctcggaaagc ggccgcgcca cgcgctatct ggccatcgcg tacgcgccct
     3181 tcttcggcct ggcgtcgccg gccgggctcg acgacggccc cgacgccgcc ttcatggcgc
     3241 gacatctcca gcgctcgttg aaatcgctca aggtgacgcc ggccgtgcac ccgttgctgc
     3301 gcgggacgcg caagctgtcg gagcgggtcg gggtgggcga gcctgtgacc aacgcgctgg
     3361 gcgcgatcgt gctctcgtgc ttcttcgtgt ggtggtggca gcgccgacgc ggcccgcgcg
     3421 ccgcgcgcga attcgacttt ctgctgttgt tctcggtgat gatgatcgcc gcgtactcga
     3481 cctacgtgtt cggggtgttc ttcttcctgc gctattacta cccgctctac ttcgtcggga
     3541 tgatcttcgc cgggctcgcg atcgacgacg cggccgcgtg ggtgcgcgcg cgctcgattg
     3601 gggtgcgccg cgcggcgctg gtggcgagcg gcgtgtacgc ggcggcgctg ctgtacatgg
     3661 gctacacgag cgcgttcagg acgatccgcg tttacgggtt ctacgacgcc gcgcgctggg
     3721 tgaccacgca cacggatccg tcggagacga tcggcgtctt ccagggcggt gcgatcggct
     3781 atctgtcgca gcggcgcgtg gtcaatctgg acggcaaggt gaacaccgac gcctaccagg
     3841 cgctgcgcgc ggggcgcttg ggcgagtacg tgcacgaggc gcacatcgac ctggtgctcg
     3901 acagcgagcg cgtgctcgac ctgttcctgg gtccgtggtc gaaggaggac cgcgagcgca
     3961 tcgcgcacga tacggtcttc gtgggcgccg agcacggggt tcacggctgg atcggctatc
     4021 gagtgacgcc ggcgcggggc ttcaacgcgg gctcgtcttc cacgccgggc ccgacgcgta
     4081 tgcagcccgg acgatagcag gctcgcaccg agggcttccc tccgctctca ggtcctcgct
     4141 cgcatccgat tggcaattgt ggctcgctgc ggaagttcgc gtcgggaacc ctcggtgcga
     4201 tcccttgatc accgcagcaa gcgttccatt gggtgcgtgt gacgcgcaac accgcccgcg
     4261 cgcgagagca ccggcaaaca gcggggcgtc accgaaacct ccagcggctt ttcgcgcctt
     4321 cccaaatact ccccgtcgat gtgcagtgga aacggtcgtt gagccgagac gcgcacgcgc
     4381 gtgccggtgt gaatcgacac cccgcgcttg ccgacgtgct tgccgtggat ggtgctcggc
     4441 aagagtgcga gcatgcgggc gcgcggcatg cggtcggccg tgcacacgtc gaggcgcccg
     4501 tcgtcgggaa acgcgcccgg gttcaaatag aaccctccgc cggcgcgcac gccgttgccg
     4561 atcgacacca gcaggaactc cccctcgacc acgcgatctc catcgaacga gagcgtgaag
     4621 cggttcgact cgtattcggc cagggccttc atcacggcca gcaagtagag cggaagtcca
     4681 cgaaggtgcc gaatcgagag gctgtggaag gtgaccatgg cctcgaagcc gatcccgatg
     4741 ctgttggcac agtagcgatc cccgcaatcg agaacatcga gccggtatcc cacgcccagc
     4801 tcgacgacgc gcgcgagctc ctcgggggta cgcgggcaat cgaccagcga tgcgaaatcg
     4861 ttgccggatc cggtcggaac gacgaccatg gggaccgggt tgggcatgag gccgttgacg
     4921 acctcgctca ccgtcccgtc gccgccgaag aggcaaacga tgtcggaatc cgcggcggcc
     4981 ccgcgcgcga ggtcgcgggc gtggcccggt ccttgcgtcc aaaggaggcg ggtatcgcct
     5041 cgaacggcac gggcatagcg ctccaccatg gcgcgcgttc ggccccgtcc ggcggtcgga
     5101 ttggcaatga agctgatgcg cataatcttt cacggcgacg cgggcaggcc gcgctcggga
     5161 gcaccgaggc tagcggagtc gtgcgctctc cggaagcgga atcgcctcgc gattctcggc
     5221 accgcgctgt gcgcggcggg gtgcccgtcc tcgtccacgg ccgccccgct taccgacgtc
     5281 tggcacgtcg gccccacccc cgaggtgcgc gcgatccgtc acaccggcag cgtcctgtgg
     5341 atcgggaccg cggccggtct ttacgtcgtc gacattcgca acgggggcat actgtcccac
     5401 gtgggcgcgg gtgaacagct cccctcggtg tccgtgcgcg cgatcgcggc caagggcgac
     5461 agcgtgttcg tgggcaccga cgagggacta tcgctgtttc gccgcaccga cgcgggcggc
     5521 ctcgagtcgt cggtgttcac gccgtcctcg ccgggacggc tctcggcggt cccgctgacg
     5581 cgcatcgccg gtctcggggt cggcgccaac ggcgacgtcc ttctggcgac atcgggccgc
     5641 ggtgcgggcg tcattaccaa gtcgggcggc tactccatca cgcgccgcga ttcgctgctc
     5701 gaagacaagg tcttcgcgat cgtcgatcgc gccgacggaa cccggtactt cgccacctcg
     5761 atggggttgt gcgcgcaagt caacgatacc agcttcgtct cgttccaggc cgggaccggc
     5821 attccgcgcg gcgaagtgcg cgagctggtt cccggcgagc gcaactcgtt ctacctccgc
     5881 gtcgccaacc aaggtgtgtt tcgcttcgac ggtgcgcgcg ccgtcgaagt cgactccccg
     5941 cccggggtgc ggctgcgcga cgccgcgtcg atatcggtcg gtgacgacgg cacgctgtgg
     6001 gtcgcgggcg acggatgggt gttcgcgcgt cgcgggggca agtgggcgcg cgtcccggtt
     6061 ccgaccgagg acgcgaacgc gcgctggcgc gtcgtggtgg ccgatggagt gggggcgttc
     6121 gcgggctcga gcgacggccg cgtggtcgca ctcggtcggg gcggcgcgct gcgcgtgacc
     6181 ctgccgtcgg gactccccgc cccgcgcgtg caatcgctgg ccgcggacgg cagcggcggt
     6241 gcgtggttcg tgtgcgacgg ccggctggtg cgagcggacg cgctctcgcg cagcgtcgag
     6301 gtcgagaatg gcccgcgcaa cgtgcgcgcg atttcggttg gacccgacgg cgaggtatgg
     6361 accgcggggc gctggacggt ccgacgccgc accggaagca cctggctcga cgttcacccc
     6421 gacgtgatcg aagcggaccc ggcctacacg tccttgtacg tcgacgagat gggagcctgg
     6481 gtcgggacgc gctccggcgc gctgtatcgc tacgacggga gcgtttggct ccgtctggcg
     6541 cgcgcgccga atacccccga gtcgatcgcg gccattcatt ccgccgacga gggcgcgtgg
     6601 gcgctgacgg gcggccggct ggcccgaagc cacgacggcg gctggcgccg ctatcccggc
     6661 atcgacagca gcgccgcggt cgtcgacatc gcgagggcgc ccaacggaac ctggctcgcg
     6721 gccaccgaaa cccgcatgtt cgtgttcgaa gaaaccaagc gggcgtgggt gcccgcgcgc
     6781 gagtggagct cgaagacctc ggccgggctc gggccgttgc acggacggat gcgcgcaatc
     6841 gcgttcgacg ggcaggggcg cctcctggtc ggtacgaccg agggtgtggc gctgtgcgcg
     6901 ccgagcggcg tgcgatggct cacggcggcc gacgggctcg ggggcgggga ggtggccgac
     6961 ctggcggtag acggcgaccg gctgtggatc ggcttcgcgg aaaatggggt ttcggttatt
     7021 tcgacccgtg ggttatggtg acgccgggag cgctcccggt ggggaaaagg gtttggtggc
     7081 ctcgaaggcc tgtgctaaga tgacacttgc cccccaactc tggaagaggt cagcgaagag
     7141 ccactgatat cgcctccatt agacgcgccg tcgcggcttg ccgtgccgac gcggagattg
     7201 catccaaggt gtcccgtcct cgggagttga gtatcgatgt ggcagttgct gttctgggtt
     7261 agcctcgccc tactcgttta catctacgtc ggttacccgg tcgccttggc ggcgctgggc
     7321 cgtttgcgcg cgcgcgcgcg gtccacgcct ccggacagcc cgctcccatc ggtgtgcctc
     7381 atcatctcgg cccatgacga agaagcggtg atccgcgaga agatcgagaa cagcctgcgg
     7441 ctggtgtacg acggcctcct gtcggtcgtg gtcgcctcgg acgggtccgc cgatcgcacc
     7501 aacgagatcg cgcgcgagta ccacgatcac ggtgtcgagc tgatgcactg gccggcccgc
     7561 cgcggcaaga gcgcggtctt gaacgaagtc gtgcgcgcgc gccgcgagga cgtgctcgtg
     7621 ttcaccgatg ccaacgcgct cttcgcgccc gacgcggtgg cacggctcgc ggagcgcttc
     7681 cgcaccccgt cggtgggctg tgtggtcggg gagctcaagt acgcgcgcga cgcgagcacg
     7741 gtcggacagg gcgagagcct gtattggcgc tacgaatcgc tcgtgaagtc gctcgagagc
     7801 cgcatcgaga gcgtgctggt ggccaacgga gccatcttcg ccgcccgccg atcctgggtg
     7861 cgcgagctct tcctcgacgt cgccaacgac ttccagattc cgttcgacgt ggccgcccgc
     7921 ggtgcgggcg tggtctacga accctccgcg gtggcgatcg agcgcacggc cgagttgtgg
     7981 gaagaggagt tcggtcgcaa ggtgcgcatc gtgctgcgcg gtattaccgg ctacgtgaga
     8041 ttgcaccgcc gcatgcacgc gctgcgcgcg tggcagttcg tgagccacaa gctgctgcgc
     8101 tggagcgtgg gggtgatctt gctggtggtg ctcgcgtcga gcgtagcgct ggcggggtcg
     8161 tcgccgtggt tcgccgcctt tctcggtctg cagctcgtct gttacgccgc ggctgtggcg
     8221 gcgtggctcg tgcgacggcg ccgagacgtg ccccgcgtcc tctacgtgcc cttctatttt
     8281 acgatggtca atgccgccgc gctggtcgcg ctgggccgct tcgtcgcggg cggacgccaa
     8341 gcgacgtggg aaaaggccgc cagcacgcgc gccactcccc cccgcgggtt agaggcgggc
     8401 ggggtgggtt cgatgccggt ggcgatggcg gaacgcggcg cgcggcgccg cctcgtcgag
     8461 aagtaaaccc cctttcgaat gcttttcttc tttcagacac tgatccggtg gcggaggttc
     8521 atcgtggcga gcgcggttgc gtccgcggcg gtgcttggcg gcgtgagctt cctcctgccc
     8581 gagtggtaca cggcccggac gtcgatcttt ccgcccgagc cgacggtggc gatgccgatg
     8641 tacgcggagc tggtgcagca gctctcggcc ccgttgctgg gcccgatcgc gaccggagcc
     8701 gcgcccgaga ccatctacat cgagatggtc aagagccgca gcgtgggcga gaaggtgatc
     8761 accgagttcg acctcatgaa tgcgtacaag tccgaacgca tcgaggactg cctcgaggag
     8821 ttgcattcgc acctgggctt cacgctgctc gagaacggca tgctgatcgt cactttcgag
     8881 gatcgcgacc ccgagcgcgc ggcggcgatc gcgaaccgct tgatcgagct gatcgacgag
     8941 accacccgcg gcctcaaggt ctcgcgcgcg gggcggacgc gcggcttcat cgagcgccag
     9001 ctggtcgatc gcgagcggtt gctcgcccaa gccgagacgg acctcaagga gttccaagag
     9061 gagttcaata ccgtcgacct cgacgagcag ttgcgctcgg ccatggacat cgtgacctcg
     9121 ctctcggcgc gggccatcgc gctggagacc gagatgcgca tcatgtcgca ctacacctcg
     9181 aagacctccg aggagtacat ccgcaagcag aacgagtaca acgaggtggt gtcgcagttg
     9241 agtaagctca agtcgaacgg cgaccaggcc gaccgcgaca tggtgcgatc gtacataccc
     9301 gcactggccg acgtccccga cgtggcgctg aagtacattc gcctgcggcg cgcggtggaa
     9361 gtccagacga ccgtctacac catgctggtc agcgagttcg agaaggcgcg catcgaggaa
     9421 gcgcgcgaca caccggtggt gcaggtgctg gacacggcgg ccccgcccag cctgcgcagc
     9481 cggcccaaac gcaagctatt cgttttggtc gggttcctgg tgggcgtggc gtggagctcg
     9541 ttcgtcgcgc tgttcaacac cgcctggcgc gacagcctcg gcaaatcgtc cgtggtgcgc
     9601 cagctgctcg agccgctggc cggtgacctc gcgcgcctgc gacggcgcgc caagacgccg
     9661 tgatctcggt cgccgcgcgc gagcgcgcga ccctattcgc gatcgcgctt tttccagtcc
     9721 tcgccttcgt cgcgcttcga ctgttcggct acatgaagtt cgcgcgcctg ggcgcgatcg
     9781 cgctggccgc ggtggcggcc gccgccgtgg tgttcctgcg cccccgctgg gggctctacg
     9841 cgctcgtctt ctacatctat tcgggcgcgg gattgttcct gcccattcaa ctcgccgcgc
     9901 ccctgatctc gatcgtgctg gcggcggtgc tgctggactg ggttcgcggg caagtacctg
     9961 gtttgccggg cgcgtacttc tgggtctcgg tcggactgtt cgtcctcgcg gcgctgaact
    10021 ccacggtggt ggcgcgcgac atggccgcgt cgctgcgcga catcggcacg ttcctcaagg
    10081 ttctcctggt ggtggtgctc gccgttcacc tcattcgaac gcccgaccag ctgcgcgcgt
    10141 tgtgctacgt cgtgttcgcg ggggccgtgg gcacggtcgc gctgggggtg ctcgccatcg
    10201 cgatcgggtt ggagggcgcg ggcgaaaacc agatctacgg ggtgcagatc atgcggttca
    10261 cgggcgccca cgtcgacccc aacaaggcgg ccgcgtacat gtgctccgcg cttccgctgg
    10321 ggatcttcgc ggtgcgccac tgccacagcc ggctgacgcg catcgctttc atcctcgcag
    10381 tcgtcgtgct cgtcgtgggc atcttcgcca cgctgtcgcg cagcgtattc ccgtcggtcg
    10441 ccgtcgtgct gttgggagtt attctgtacg agattcgcag ccgccggtcc ttcgtcgtgc
    10501 tggtggcgat tctcgcgttg ggcgtggtgc tggcgccgcg ctacta
//
LOCUS       DASPGK010000358         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_87253, whole genome shotgun sequence.
ACCESSION   DASPGK010000358 DASPGK010000000
VERSION     DASPGK010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87253"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1145
                     /locus_tag="VEC56_09965"
     CDS             357..1145
                     /locus_tag="VEC56_09965"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HXV14517.1"
                     /translation="MTPAPEYNATIVRREDLHGGLMMLWVAPDGGEFAPFWPGQFVAI
                     GRIVDASGAPRSLVKRSYSIGSSAHDRHAVQLFVVHVDDGEFTSWLFAQREGARVWLA
                     PKASGGFTLQGFERGRDLALVATGTGVAPYVSMVRTHQNAPPWRRAVIINGVRVAADL
                     GFRGELEQAAAREPRLVYLPATTREPADSAWTGLRGRVGDLLEPGRFRALAGFDLDPG
                     RCHVYVCGNPSMIEDLERLLTARGFKKHTPRHPGTLHLEKYWTE"
     gene            1374..>3945
                     /gene="aceE"
                     /locus_tag="VEC56_09970"
     CDS             1374..>3945
                     /gene="aceE"
                     /locus_tag="VEC56_09970"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXV14518.1"
                     /translation="MDELRQETREWLESLDYVYQTEGAARVQELLQRLQDRATSYGVR
                     IQFPGNTPYINTIPASKQPPYPGDRIIERRIKSVIRWNAMAMVVRANREDPGIGGHIS
                     TFASAATLYEVGFNHFFRGKDHPEGGDIIYYQGHAAPGIYARAYLEGRLTEKHLENFR
                     HELATEGGISSYPHPWLMPDFWEYPTVSMGLTALLAIYQARFNKYLENRGVLEASARR
                     VWAFLGDGEMDEPESLGALTLASRERLDNLVFVINCNLQRLDGPVRGNGKIIQELGAA
                     FRGAGWNVIKLITGGDWDPLLAADSEGVLVKRLSEVVDGQFQKYVTESGAYIRKDFFG
                     ADPRLLKLVDNLSDEQVRNLRRGGHDPEKVYAAYKAAVEHKGLPTVILAWTIKGYGLG
                     ESGEGKNITHQQKKLNEDELRAFRNKFGIPISDDDVAQAPFYKPPADSEDMQYLRARR
                     EALGGFIPERRDTAPPLETPPASIFKDMYGGSGDREVATTMAFVQMLSTLLKDKGIGK
                     YVVPIVPDEARTFGMESLFRQVGIYSSVGQLYEPVDKETLLYYKEAVDGQVLEEGITE
                     AGSVASFIAAGTAYATHGINMVPFFIYYSMFGFQRIGDFIWAAGDMRCKGFLLGGTSG
                     RTTLAGEGLQHEDGHSHILALPHPTLRAYDPTFAYELAVIIHDGIERMYKNNEQLFYY
                     ITVMNEPYKMPAMPEGSREGILNGMYRLYASGKGDTKNKVHLFGSGAILNEVIRAQRI
                     LEDAYDIPADVYSVTSYKQLYADAIECERHNLLHPGEDEKVPYVAQVLSGTKGVYVAA
                     SDYMKALPASIARWMPAGFDFLGTDGFGRSSNRAGLRDFFEVDARYIALAALRALARE
                     G"
BASE COUNT          706 a         1280 c         1313 g          646 t
ORIGIN      
        1 tggcggggtt tgatactgcg ctacggcatc ggccacccat gtcatcagaa tgaacgtaga
       61 gaaggccgcc cagagcgcca cggtgcgttt cattcagtcg ctcccaaagt aatgcgatat
      121 gtctgcgtga gaagagtcgg agtcggcggc tcgattacac cctcgcgccg caatcgccgg
      181 cggagtccga ggggcgccga cgaaaatatc gccctagttt atcggtaagt ggtccaaagt
      241 caaggccctt tatccccgct cgggggcgcg gctccgggta tcgcaaccgc ggcccgcggc
      301 gatccggcgc tgttcttggc cgcccgtcgt gtgctagcgt ggggcgcatt catcccatga
      361 cacccgcccc cgagtacaac gccaccatcg tccgccgcga ggacttgcac ggcggactca
      421 tgatgctgtg ggtggcaccc gacggcggcg agttcgcgcc cttctggccc ggacagttcg
      481 tcgcgatcgg gcgcatcgtc gacgcgtccg gcgccccgcg ctcgctggtc aagcgttctt
      541 attcgatcgg gtcatcggcg cacgatcggc acgctgtcca gttgttcgtg gtgcacgtcg
      601 acgacggcga attcacctcg tggcttttcg cgcagcgtga gggtgcgcgc gtgtggctcg
      661 cccccaaggc gagcggtggt ttcacgctgc aaggattcga gcgcggacgg gacttggcgc
      721 tggttgcgac cgggaccgga gtcgcgccgt acgtgtcgat ggttcgcacg caccagaacg
      781 caccgccgtg gcgtcgcgcg gtgatcatca acggcgtacg ggtggccgcc gacttgggat
      841 ttcgcggcga gctcgagcag gcggccgcgc gcgagccgcg tctggtctat cttcccgcga
      901 cgacgcgcga gcccgccgat tcggcgtgga cggggctgcg cggtcgcgtg ggcgacctgc
      961 tggagccggg acgctttcgc gcgctcgcgg gcttcgatct cgatccgggt cgttgtcacg
     1021 tctacgtgtg cggcaacccg tcgatgatcg aagatctgga gcggttgttg accgcccgcg
     1081 ggttcaaaaa gcacacaccc cggcatccgg ggacgctgca cttggagaag tactggacgg
     1141 agtgagcgag cgcgacgcgc gggccgccgc gggccgattg acgcggcccg gtataaggct
     1201 tatcgtagcg gtaagccatg agcacgacgc gagatcggaa gtcgaccggg gcgcgcgcgg
     1261 agagcgagga acccgctccg cgccgcgtcg aagcggtatc gccggagggt gcgccggccg
     1321 ccgagcccga gccgactccg cccgcatcgg atgaaccatc ggaagcgctg aacatggacg
     1381 agcttcgaca agaaacgcgc gagtggctcg agtcgctcga ctacgtgtac cagaccgaag
     1441 gggccgcccg cgtgcaggag ctcttgcagc gcctgcagga ccgcgccacg tcctacggcg
     1501 tgcgcattca gttccccggc aacacgccct acatcaatac gattcccgcg tcgaagcagc
     1561 cgccttatcc cggcgaccgc atcatcgagc gtcgcatcaa gagcgtgatt cgctggaacg
     1621 cgatggcgat ggtggtgcgc gccaaccgcg aggaccccgg catcgggggc cacatctcga
     1681 ctttcgcgtc ggccgcgacg ctctacgagg ttgggttcaa ccatttcttc cgcggcaagg
     1741 accaccccga gggcggcgac atcatctact accagggtca cgccgcgccc ggcatctacg
     1801 cgcgcgcgta cctcgaggga cgcctgaccg agaagcacct cgagaatttc cgccacgagc
     1861 tcgcgaccga aggcggcatc tcgtcgtatc cgcacccgtg gctcatgccg gacttctggg
     1921 agtaccccac cgtgtcgatg ggcctgaccg cgctgctcgc gatctatcag gcgcgcttca
     1981 acaagtatct cgagaaccgc ggggtcctcg aggcatcggc ccgccgcgtg tgggctttcc
     2041 tgggcgacgg cgagatggac gagcccgaat cgctgggggc gctcacgctc gcgtcgcgcg
     2101 agcggctcga caacctggtc ttcgtgatca actgtaattt gcagcgcttg gacggacccg
     2161 tgcgcggcaa cggcaagatc atccaggagc tgggcgcggc cttccgcggc gccggctgga
     2221 acgtgatcaa gttgatcacg ggcggcgatt gggacccgtt gctcgccgcc gacagcgagg
     2281 gcgtgctggt caagcggctg tcggaagtgg tggacggaca attccagaag tacgtgacgg
     2341 agtccggcgc gtacatccgc aaggacttct tcggcgccga cccgcgccta ttgaagctgg
     2401 tcgacaacct ctccgacgaa caggtgcgca acttgaggcg cggcggccac gaccccgaga
     2461 aagtgtacgc ggcgtacaag gccgcggtcg agcacaaggg actgccgact gtgatcctcg
     2521 cgtggaccat caagggctac ggcttgggcg agagcggcga aggcaagaac atcacgcacc
     2581 agcagaagaa gctcaacgag gacgagttgc gcgcgttccg caacaaattc ggcattccca
     2641 tctcggacga cgacgtggcg caggcgccgt tctacaagcc gcccgcggac agcgaagaca
     2701 tgcagtacct gcgcgcgcgc cgggaggcac tcggcggttt catccccgag cgccgcgaca
     2761 ccgcgccgcc cttggaaaca ccgcccgcgt cgatattcaa ggacatgtac ggcggcagcg
     2821 gcgaccgcga agtcgccacc accatggcgt tcgtgcagat gctctcgacg ctcctgaagg
     2881 acaagggaat cgggaagtac gtggtgccca tcgtccccga tgaggcccgc accttcggca
     2941 tggagtcgct gttccgccaa gtcggcatct actccagcgt ggggcagctc tacgagccgg
     3001 tcgacaagga gaccctcctc tattacaagg aggccgtcga cgggcaggtg ctcgaagagg
     3061 ggatcaccga ggccggatcg gtggcgtcgt tcatcgcggc ggggacggcg tatgccacgc
     3121 acggcatcaa catggtgccg tttttcatct attactcgat gttcggattc cagcgcatcg
     3181 gcgacttcat ctgggccgcc ggcgacatgc gctgcaaggg cttcctgttg gggggaacct
     3241 cgggacgcac cacgctggcg ggcgagggat tgcagcacga agacggccac agccacatcc
     3301 tggccctgcc gcaccccacg ctgcgcgcgt acgaccccac gttcgcgtac gagctggccg
     3361 tcatcattca cgacggcatc gagcgcatgt acaagaacaa cgagcagctg ttctactaca
     3421 tcacggtcat gaatgagccc tacaagatgc cggccatgcc cgaggggtcg cgcgagggaa
     3481 tcctgaacgg catgtatcgt ctgtacgcgt cgggcaaggg cgacacgaag aacaaggtcc
     3541 atttgttcgg cagcggcgcg attttgaacg aagtgatccg cgcccagcgc atcctcgagg
     3601 acgcgtacga catccccgcc gacgtgtatt cggtgacgag ctacaagcag ctctacgccg
     3661 acgccatcga gtgcgagcgc cacaatttgc tgcaccccgg cgaggacgag aaggtcccgt
     3721 acgtggcgca agtattgagc ggaaccaagg gcgtctacgt cgccgcgtcg gactacatga
     3781 aggcgctgcc ggcctcgatc gcgcgctgga tgccggccgg cttcgatttt ctgggcaccg
     3841 acggcttcgg gcgctccagc aaccgcgccg gtttgcgcga tttcttcgaa gtcgacgcgc
     3901 gctacatcgc gctggccgca ctgcgcgcgc tcgcgcgcga gggcc
//
LOCUS       DASPGK010000359        12876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1140577, whole genome shotgun sequence.
ACCESSION   DASPGK010000359 DASPGK010000000
VERSION     DASPGK010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140577"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /gene="thiC"
                     /locus_tag="VEC56_09975"
     CDS             <1..1369
                     /gene="thiC"
                     /locus_tag="VEC56_09975"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXV14519.1"
                     /translation="TNGLSHQHPGDPRGARIPRLVTPEFVRDEVAAGRAIIPANVNHP
                     ELEPMVIGRNFRVKINANIGNSAVASSIEEEVAKMRWATLWGADTVMDLSTGKNIHET
                     REWILRNSPVPIGTVPIYQALQKVGGKPEELSWEVFRDTLVEQAEQGVDYFTIHAGVL
                     LRFIPWTAQRATGIVSRGGSIMAAWCLAHHRESFLYTNFDQICEIMRAYDVSFSLGDG
                     LRPGSIADANDRAQLGELQTIGELTTKAWTHDCQVMIEGPGHVPMHLVQENMELELEH
                     CHEAPFYTLGPLTTDVAPGYDHITSAIGAAMIGWHGCALLCYVTPKEHLGLPNRDDVK
                     AGVIAYKIAAHAADLAKGFPGAQEWDNALSKARFEFRWEDQFNLAMDPVTARAFHDET
                     LPKDSMKTAHFCSMCGPHFCSMKITEDVRAYAERHGMTEAQAIEAGLAEKAREFKDGG
                     AKIYR"
     gene            1465..3039
                     /locus_tag="VEC56_09980"
     CDS             1465..3039
                     /locus_tag="VEC56_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14520.1"
                     /translation="MRFVLVAAIAAAIVVPAEGATLSGFATHNGKRAVGAFVTARDTT
                     RGVSWSVRTGPDGTYRLELAEGAYVASARTRGASASTQPVGLASTATADFALSSPVDA
                     ALDAPSRAYLGLLPDGEEKRRFAVDCLGCHALNARVVLDSTGEPWDEAGWKKSTDKML
                     SFAGHTTHFPILPPDRASEPTARFVARYLTSQAIAGACASTEPVGMPATSYTVTEWDL
                     PDQNDFPHDLMRDGRGGILITGMFSGLMYVLDPKTHAFSTVEIPVPMANPRALDVDKN
                     GDWWILCGFPNKIARRRASAGTWDVFDIGVYGHSVGVDAHQRAWFNGHFTRDPIRFGY
                     VDGTTGKTHTLDAPATAMPPEQGSPIPYELRVGPDGTVWMTELAGNRLLRHDPGTGAT
                     KAYPLPSPHSGPRRCDVSPDGTVWVPEFAGGKLARFDPKTERFEEFDLPVHDTLPYCA
                     RVDPRRGVVWVSLCAADAIARFDPATKTFALFELPTRNAFIRHLDVDPESGEVWAAYS
                     HSPNLEMRIARLRVAN"
     gene            complement(3128..4765)
                     /locus_tag="VEC56_09985"
     CDS             complement(3128..4765)
                     /locus_tag="VEC56_09985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV14521.1"
                     /translation="MNKENSMHHDIRRLGRCLAVAAMAAFVAALPAHAGQKQDQKEKA
                     KRDAEYEQIQSYIYDKRYEQALAAAQAFMKKYPVDELAEAANFWSCYALDRTTEDYGK
                     AFDCYRAFLQAYPDGKWADDATIEYAKLAKRLAKAGDPRGKAALEALRERGDDDEEDA
                     DFKLSVLYALKDVDREAALQATKDLLRSRDADVRKRAVFVLADFEDQAALDQLIAVAK
                     TDPDPDVRRHAIYAISEYGDNPKVIDAMVVIMQAEKDPDIRRHVLYAIAEVDRPDVTK
                     ILVDVAMKDPDVELRTAATYALAEVDSPDAARALEKLAADAPTFDMKRAALYALIERD
                     DADIVPLLKKLALSKDKGSEAAELRRAAVYALAEVDDPQVIPILNEVVKSSDDAEVRQ
                     AAFYALAEHGGPAAVDALKTAALDTKNQEIALAAVWGLSDLLDEGEGAFFMDVYQKSP
                     FEEVRRAAFHCMIDHGGDASASALGAMLESETNNDQRRLIVWALGDIETDESVAILAK
                     VARTDKSRETRQIAVQALGEIGTPAAKKALRDLLNDE"
     gene            complement(4777..5703)
                     /locus_tag="VEC56_09990"
     CDS             complement(4777..5703)
                     /locus_tag="VEC56_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV14522.1"
                     /translation="MTTCDRCQAWMVEALYGELEDSRRPEFDAHLAGCAECTALLSQL
                     QTTARLMSRRRRPDPGPEFWAGYQHRLEERVAREDTVVDSSRFARRRSLGSWGYRVAA
                     VVALLAVGAWLGRTVLGPSGSETPMLATTRADSMPREQLSPAPVDDGVVSEEPMVAET
                     NREANPMPIDAPAEEAPRAADGTGESAVVLASDEDDASRYIRHSQMLLLAMLNGEPAD
                     SVAFDAQKQRAGELVRAASSVREGSDDRRVRELVAQLELILREIAHLEQSSDVDAVEV
                     IRSRVDREGVLLRINVEQMRSDAPRATSGAID"
     gene            complement(5700..6293)
                     /locus_tag="VEC56_09995"
     CDS             complement(5700..6293)
                     /locus_tag="VEC56_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV14523.1"
                     /translation="MHDESPQLVERLRAGEPAAFREMVECYRRDVYALAYDLCGNHHD
                     AEDLSQEVFIKAFRGIGSFRADARLGSWLYRIAMNAHIDNKRRKPVQLVSIDAHESAD
                     EQPRERVLELAAVDPRPDQTAGAARFQTDVEHALDALSVQERTVFVLRHYHDMKIGEI
                     SESLSIAEGTVKSLLFRSMRKLRDRLSHYRDEVGDLA"
     gene            complement(6294..7118)
                     /locus_tag="VEC56_10000"
     CDS             complement(6294..7118)
                     /locus_tag="VEC56_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV14524.1"
                     /translation="MKHTIVTLAQLVLISAVLATPVASKGEKKTGHNAPETEVIKRVY
                     DVGKNARLSLANLNGTATITGWDKNTIEVTATKRANARERLADATVQFDLLDDHLRVE
                     VVYDFGHGDDFNGNDGLVSVDFEIRVPRHVDIRRVELVNGGIDVSDLTGDVRVSSVNG
                     DVMGEKLSGETRLSTVNGDVSLSSVKGHDSIELSSVNGSVTLYLPPNANAKLSASTVH
                     GDIRGDLGHGVTHAGSSIDAVLGNGGPRIGLGTVNGDIRIRRAGSEQRGDVDDDSD"
     gene            7356..10184
                     /locus_tag="VEC56_10005"
     CDS             7356..10184
                     /locus_tag="VEC56_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXV14525.1"
                     /translation="MRNLLRSFGLLILFPAAAWAGPDVDLQAMRTEPAPGTGTYTAPF
                     WPDGTYRSDVRSPSEFLGYPIGSVPAPHADIVRYFEYLDQLPTAELHTYAQSYEGRTL
                     VYLVIASEQNAARLSEIKADCQKLADPRTLGGAKPSDIVARTPAVAWMAYGIHGDELS
                     SCDAALELAYQLTAGTDAATRRILDNCVVVIDPTENPDGRTRWLTQLAQWNGVITSHD
                     VQSMSHTGLWPYGRTNHYLFDLNRDWFALVHPETRGRTAAILDWMPHYLLDCHEMGPL
                     DTYLFSPPREPFNPHMVGYIHKWWEKVAKEHAAQFDRFGWSYYTREWNEELYPGYGSS
                     WAIYLGAVGFLFEQAGVDGSQIKRPDGTVMTYRETVHHQFVGSMANLTAVADGRAELL
                     NDYHRQKVDNLRAKPRTFVFAAGGNRTRLDRFVEKLQHQHIEVERTTQGMKLSRARAW
                     DGTEARDVTIPAGSALVRTNQPMKQLAEAILEFDIRIPTSFLKTEKKDILARGDSRLY
                     DTTGWSLPLAYGLDAYVVDGAPRVDSEAYAIAAPAGSVSNDAAKVGFAFDGRDDRALE
                     LVARLFENQIRVWCAQKPFRAGETVMPRGSFLVRRAGNPGLDVTKLRALAEAAGVVVI
                     GVDGGLASGAYADLGGNEFSLLVAPRIALVAGTGTNSTSFGATWHLLDSRFRVRTSIV
                     DVTRVADTDLSKYNVIVLPDYSGGVEAYRGVLRKSGIENLKTWVEAGGTLVADGAGAA
                     MLADTSVAIASVRMRDQVLKDLPDYVASLALAKSAFSPVVDSLSLWGPGAAKKPAQAE
                     EKKDEKESGDLESRKREDEVARKLAPRGAIVAVDLDEEHWLAFGCPKTIPALLNTNRT
                     FVTKATQVPARLAPADRLRLSGLFWEEARARWSESVYAARDAVGSGQVIVFVSTPNFR
                     GYFHGAERLLLNALLLGPGCGTSQRVEW"
     gene            10282..10884
                     /locus_tag="VEC56_10010"
     CDS             10282..10884
                     /locus_tag="VEC56_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14526.1"
                     /translation="MSAFNRRNGIVALAVAAVAAAALASAADPPTATGGAPPDVRRIE
                     KFVHIAAFLNLAQGTVRMVAVLPIDGESSAAVLDTVAAVIRGNPSKRLRAYVVLRGSD
                     SSLRAAVLAGRAADPRIVFFWDPAGTATAAWRADPARAGVWLYDTSARFTGGPPGASL
                     VVGAPAATPEAPLAGPALREQAGELVRRVEAKMAQADDGS"
     gene            10944..12326
                     /locus_tag="VEC56_10015"
     CDS             10944..12326
                     /locus_tag="VEC56_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HXV14527.1"
                     /translation="MHTQSKPVFREILVAGVLVAALAPAAWAARGDITLVGQIKPTGS
                     DQFCNVWGFERDGRDYAVVGDWDSGPIIIDVTDPTNPFVRKVITGTGVFGFDVKVWGN
                     YIYCCEGGFGHTGASSRAIDITDFDNPIISPAFTNAHALAIHPDGYLFAEIPGLRCYD
                     LNADPTPAGYLWYDGTSGGHDSTVDVEHDRLYDFHGYDGVTRIWDISNINSRQLLGSI
                     TDPTINYHHSGDATEDGNYLYVCDELATGLEKDLTVWLISNPAAPVKVGSLFDPVSTI
                     HNLYIIGNFAFVSHYSAGFRVYDVSNPAAPELLDTYDTAPLRNGDGYNGCYGVYPFGP
                     GGIVYASDSDNGLYLFAVEDFAGVPSSVVATPGAKTARLVGNYPNPFNPSTTIAYEVA
                     REGDVSLAVYDVDGALVRVLARGRAPAGRHETRWDGADGGGRRVASGVYFVRLAANGA
                     VDTGRLVLLK"
     gene            12355..>12876
                     /locus_tag="VEC56_10020"
     CDS             12355..>12876
                     /locus_tag="VEC56_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14528.1"
                     /translation="MKPLSAHTPRAAATMAAGFAMVAGTAASAAVDVQLVGRTNLPNS
                     IFLVNVVGYVDQTTLREYAIVGDNHDKVYIVDVTDPADMRITAQIAGIPGFDVKTWGQ
                     YLYTCDGNTGGNDSRIVDILDPANPIVLPDGFASAHTFQISSAGILFAEFPGLRIYDL
                     NTTPTAPQLRHESG"
BASE COUNT         2067 a         4478 c         4153 g         2178 t
ORIGIN      
        1 gaccaacggg ctctcccacc agcacccggg cgacccgcgc ggcgcgcgca tcccgcgcct
       61 ggtcacgccc gagttcgtgc gcgacgaggt ggcggcgggg cgcgccatca tcccggccaa
      121 cgtcaatcac cccgagctcg agccgatggt catcgggcgc aacttccggg tgaagatcaa
      181 cgccaacatc ggcaactcgg cggtcgcatc gtccattgaa gaagaagtcg cgaagatgcg
      241 gtgggccacc ctgtggggcg cggacacggt gatggacctg tcgacgggca agaacatcca
      301 cgaaacgcgc gagtggatcc tccgcaactc gccggtgccg atcggcacgg tgcccattta
      361 ccaggcgctc cagaaagtgg gcgggaagcc ggaagagctg tcgtgggagg tcttccgcga
      421 cacgctggtc gagcaggccg agcagggcgt cgattatttc accatccacg cgggcgtgct
      481 cttgcgcttc atcccgtgga ccgcgcaacg cgcgaccggc atcgtgtcgc ggggcggctc
      541 catcatggcc gcgtggtgcc tcgcgcacca tcgcgagagc ttcctctata cgaacttcga
      601 ccagatctgc gagatcatgc gcgcctacga cgtctcgttc tcgctgggcg acgggcttcg
      661 acccggctcc atcgcggacg ccaacgatcg cgcgcagttg ggcgagctcc agaccatcgg
      721 cgagctcacg acgaaggcgt ggacgcacga ctgccaagtc atgatcgaag gtcccggcca
      781 cgtgcccatg cacctcgtgc aggagaacat ggaactcgag ctcgagcact gccacgaggc
      841 gccgttctac accctgggac cgctgaccac cgacgtggca cccggctacg accacatcac
      901 gagtgcgatc ggcgcggcca tgatcgggtg gcacgggtgc gcgctgctct gctacgtcac
      961 tccgaaggag cacctcggcc ttcccaatcg ggacgacgtc aaggccggtg tcatcgccta
     1021 caagatcgcc gcgcatgcgg ccgacctggc caaggggttc cccggcgcgc aggagtggga
     1081 caacgcgctc tccaaggcgc gcttcgagtt tcggtgggaa gatcagttca atctcgcgat
     1141 ggaccccgtg accgcgcgcg ccttccacga cgagacgctg cccaaggaca gcatgaagac
     1201 ggcccatttc tgctcgatgt gcggaccgca cttctgctcg atgaagatca ccgaggacgt
     1261 gcgcgcctac gcggagcgcc acggcatgac cgaggcgcaa gccatcgaag ccggcctggc
     1321 cgagaaggcg cgcgagttca aggacggtgg ggccaaaatc taccgctagg ttgcatcgcg
     1381 cgccgagggt cccgcaaccc gtaccgcgtc gggtccgtac taccgggcca accccacttt
     1441 ccccctctcc ctgaggaggc acccatgcgc ttcgttctcg tggcggcgat cgctgcagct
     1501 atcgtcgtgc ccgcggaggg cgccacgctt tccggattcg ccacgcacaa tgggaaacgc
     1561 gccgtcggcg cgtttgttac cgcgcgcgac accacgcgcg gcgtgtcgtg gtcggtgcgg
     1621 accggtcccg acgggaccta tcgactcgag ctcgccgagg gcgcgtacgt cgcctcggcg
     1681 cgcacgcgtg gtgcgtcggc atccacgcag ccggtcgggc tcgcctcgac cgcaaccgcc
     1741 gacttcgccc tgtcgtctcc cgtcgacgcc gccctcgacg cgccgtcgcg cgcctacctc
     1801 ggcctcttgc cggacggaga agagaagcga cgcttcgcgg tcgattgcct gggctgccac
     1861 gcgctcaacg cgcgcgtcgt gctcgattcg accggcgagc cgtgggacga agcgggctgg
     1921 aagaaatcca ccgacaagat gctctcgttc gcgggtcaca ccacgcactt ccccatcctc
     1981 ccgcccgatc gcgcgtcgga acccaccgcg cgcttcgtcg cgcgctacct gacctcgcaa
     2041 gcgatcgcgg gcgcgtgcgc gagcacggag ccggtcggga tgccggccac gtcgtacacc
     2101 gtgaccgagt gggacctccc cgaccagaac gatttcccgc acgacctcat gcgcgacgga
     2161 cgcggcggga tcctcatcac gggcatgttc agcggcctca tgtacgtcct cgacccgaag
     2221 acgcacgcgt tctcgaccgt cgagatcccg gtgccgatgg ccaatccacg cgcgctcgac
     2281 gtcgacaaga acggcgactg gtggattctg tgcggcttcc ccaacaagat tgcgcgccgg
     2341 cgcgcgagcg cggggacgtg ggacgtcttc gacatcggcg tgtacggaca cagcgtgggc
     2401 gtcgacgcgc accagcgtgc gtggttcaac ggacacttca cgcgcgaccc gatccgcttc
     2461 ggctacgtgg acggcacaac cggaaaaact cacacgctgg acgcgccggc gacggcgatg
     2521 ccgcccgagc aagggagtcc gattccctac gagctgcgcg tgggaccgga tggcacggtg
     2581 tggatgacgg agctcgccgg caaccgcctg ttgcgccacg atcccgggac cggcgccacc
     2641 aaggcgtacc cgctgccgtc tccgcacagc ggcccgcggc gttgcgacgt ctcccccgac
     2701 ggcacggtgt gggtgcccga gtttgcggga ggcaaactgg cgcgcttcga ccccaagacc
     2761 gagcgcttcg aagaattcga tctcccggtg cacgacaccc ttccctactg cgcgcgtgtc
     2821 gatccgcgcc gcggcgtcgt gtgggtctcg ctgtgcgcgg ccgacgcgat cgcgcgcttc
     2881 gatcccgcca cgaagacgtt cgcgctcttc gagctgccca cccgcaacgc gtttattcgc
     2941 cacctcgacg tcgacccgga gtcgggcgag gtgtgggcgg catactcgca ctcgccgaac
     3001 ttggagatgc gcatcgcgcg cttgcgagtc gccaactaga gcgcttcgcc cgtccgctca
     3061 caacggagaa ggcccgcgtc gctggaacga cgcgggcctt cgcgctcgcg gacgatgggg
     3121 tctcgcgtta ctcgtcgttg aggagatccc gcagcgcctt cttcgcggcc ggggtgccga
     3181 tctcacccag cgcctgcacc gcgatctggc gcgtctcgcg gctcttgtcg gtgcgcgcca
     3241 ccttggccag gatggccacc gactcgtcgg tctcgatgtc ccccaacgcc cacacgatca
     3301 ggcggcgctg atcgttgttc gtctcggatt cgagcatggc gccaagggcg gacgcggagg
     3361 cgtcgccgcc atggtcgatc atgcagtgaa acgcggcgcg ccgcacttct tcgaacgggg
     3421 acttctggta aacatccatg aagaacgcac cctcgccctc gtcgagcagg tcgctcaagc
     3481 cccacaccgc ggccagggcg atttcctggt tcttggtatc gagcgcggcc gtcttgagcg
     3541 catccacggc ggccgggccg ccgtgctcgg ccagcgcgta gaaggccgct tggcggactt
     3601 cggcgtcgtc cgaggacttg accacttcat tgagaatggg gatcacctgc gggtcgtcca
     3661 cctcggccag cgcgtacacg gccgcgcgcc gcagctcggc cgcctccgag cccttgtcct
     3721 tgctcagcgc cagcttcttc aggaggggca cgatgtcggc gtcgtcccgt tcgatcaacg
     3781 cgtacagcgc ggcgcgcttc atgtcgaacg tgggtgcgtc ggccgcgagc ttttccagcg
     3841 ccctggcggc gtcgggcgag tcgacttcgg ccagcgcgta cgtggccgcg gtgcgcagtt
     3901 cgacgtcggg gtccttcatg gccacgtcga ccaggatctt ggtcacgtcg gggcggtcga
     3961 cctctgcgat cgcgtacagc acgtgacggc ggatgtcggg gtccttctcc gcctgcatga
     4021 tcacgaccat ggcgtcgatc accttggggt tgtctccgta ttcggaaatg gcgtaaatcg
     4081 cgtgccggcg aacgtcgggg tccggatccg tcttcgcgac cgcgatgagc tggtcgagcg
     4141 cggcctggtc ctcgaagtcg gccagcacga aaacggcgcg cttgcgcacg tcggcgtcgc
     4201 ggctccggag caggtccttg gtggcttgga gcgcggcctc gcgatcgacg tccttgagcg
     4261 catacaggac cgagagcttg aagtcggcgt cttcctcgtc atcgtcgccg cgctcgcgca
     4321 gcgcctcgag cgcggccttg ccgcgcggat cgcccgcctt ggcgaggcgc ttggccagct
     4381 tcgcgtactc gatggtggcg tcgtccgccc atttcccgtc cgggtacgcc tgcaagaagg
     4441 cccggtagca atcgaaggcc ttgccgtagt cttcggtggt gcggtcgagg gcatagcagc
     4501 tccagaagtt cgccgcttcc gccagctcgt ccaccggata cttcttcatg aaggcttgcg
     4561 cggccgccag cgcctgctcg tagcgcttgt cgtagatgta gctctggatc tgttcgtact
     4621 cggcgtcgcg cttggccttt tccttttggt cctgcttttg tcccgcgtgc gccggtaatg
     4681 cggcgacgaa cgccgccatg gcggccacgg cgaggcatcg tcctaggcga cggatgtcgt
     4741 ggtgcatcga gttctccttg ttcatttcca tctcgttcag tcgatagcgc cgctggttgc
     4801 gcgcggcgcg tcggaacgca tttgttctac gttgatgcgc aagaggacgc cctcgcgatc
     4861 gacgcgcgag cggatcacct cgaccgcgtc gacgtccgag ctctgctcga ggtgtgcgat
     4921 ctcgcgcaag atgagctcca gctgggccac cagctcccgc acgcggcggt catcggagcc
     4981 ctcacgcacc gacgatgccg cccgcaccag ctcgcccgct cgctgtttct gcgcgtcgaa
     5041 tgcgaccgag tcggcgggct cgccgttgag catcgcgagc aggagcattt gcgagtgtct
     5101 gatataacgc gacgcatcgt cttcgtcgct cgccaacacg acggcgctct cgcccgtacc
     5161 atcggccgcg cgcggcgctt cttccgcggg cgcgtcgatg ggcatggggt tggcctcgcg
     5221 gttcgtttcc gcgaccatcg gctcttccga cacgacgccg tcgtcgacgg gcgccgggct
     5281 cagttgttcg cgcggcatcg aatcggcgcg cgtcgtcgcg agcatgggcg tctcggaccc
     5341 ggacggtccc agcacggtgc ggcccagcca cgctcccacc gcgaggagtg ccacgacggc
     5401 ggccacccgg tatccccacg acccgagcga ccgccgtcgc gcgaagcggg acgaatcgac
     5461 gacggtgtct tcgcgagcga cccgctcttc cagccggtgc tgataacccg cccagaattc
     5521 cggccccggg tccggtcgcc ggcggcgact catcagacgc gcggtcgtct gcagctgcga
     5581 aagcagcgcg gtgcactccg cgcagccggc caggtgcgcg tcgaactcgg gacgccgcga
     5641 gtcttccagc tccccgtaga gagcttcgac catccacgcc tgacagcgat cgcacgtcgt
     5701 catgcgaggt cacccacctc gtcgcggtag tgcgacagcc ggtcgcggag tttgcgcatg
     5761 gagcgaaaca acaggctctt cacggttccc tccgcgatcg acaacgactc cgagatctcg
     5821 ccgatcttca tgtcgtggta gtggcgcaat acgaaaacgg tgcgttcctg cacggagagc
     5881 gcgtcgagcg cgtgctccac gtcggtctga aagcgcgcgg cacctgcggt ttggtcgggc
     5941 cgcgggtcga ccgcggccaa ctcgagcacg cgctcgcgcg gctgctcgtc ggcgctttcg
     6001 tgggcgtcga tactcacgag ttgcacgggc ttgcggcgtt tgttgtcgat gtgcgcattc
     6061 atggcgattc gatacaacca gcttcccagt cgggcgtccg cgcgaaaact tccaatcccg
     6121 cgaaacgcct tgatgaacac ttcctgcgag agatcctccg cgtcgtgatg gttgccgcac
     6181 aggtcgtacg cgagcgcata cacgtcgcgc cggtaacact cgaccatctc tcggaaggcg
     6241 gcgggctcac ccgctcgcaa ccgctccacc agttgcggcg actcgtcgtg cattcaatcg
     6301 gaatcatcgt ctacatcgcc gcgctgctcc gagccggcgc ggcgaattcg aatgtcgccg
     6361 ttcaccgtgc ccagcccgat gcgcggcccg ccgttgccca gcaccgcgtc tatcgagcta
     6421 ccggcgtggg tgacgccgtg gcccaagtca ccgcggatat cgccgtgaac cgtgctggcc
     6481 gacagcttcg cgttggcgtt gggcggcagg tagagcgtca ccgagccatt gacggaggaa
     6541 agctcgatcg agtcgtgccc cttcacactc gagagcgaaa cgtcgccgtt caccgtcgag
     6601 aggcgcgtct cgcccgaaag cttctcgccc atcacgtcac cgttgacgga cgacacccgg
     6661 acgtcgccgg tgaggtcgct gacgtcgatc ccgccgttga cgagctcgac gcgacgaata
     6721 tcgacgtggc ggggcactcg aatctcgaaa tcgacgctga ccagtccgtc gttgccgttg
     6781 aaatcatccc catgcccgaa gtcgtagacc acctcgacgc gaagatggtc gtccaagagg
     6841 tcgaactgca cggttgcgtc tgcgaggcgc tcgcgcgcgt tcgcccgctt ggtcgcggtg
     6901 acctcgatgg tgttcttgtc ccagccggtg atggtcgccg taccgttcag gttggcaagg
     6961 ctcaggcgcg cgttctttcc cacgtcgtag actcgtttga tgacttccgt ttcgggggcg
     7021 ttgtggccgg ttttcttttc gcccttgctc gccacgggtg tggccaacac ggccgatatc
     7081 agaaccaact gcgccagggt cacgatcgtg tgtttcatgc tcttctcctg ctcgagggag
     7141 ccgcgaggcc gactgcaacg ggtcaactgg tagatgagac ggaaaacggc cgctcggagt
     7201 ttacaagggt ttgcgccggt ttgacaccgg ctgaccgcgt tcgctattct cccgccttcg
     7261 ttccccgacg gaccggccac ctggtttcat aagaccttga ggtataacgg gttcgtcgaa
     7321 cctccggctt cctggcaaaa gcgagtgtcc aacccatgcg aaatctcctg cggtctttcg
     7381 gactcctgat cctcttccca gcagcggcct gggccggtcc cgacgtcgac ctccaagcca
     7441 tgcgcacgga gcccgcaccc ggaaccggga cctacaccgc ccccttctgg cccgatggga
     7501 cctatcgctc cgacgtccgc tcgccgtcgg agttcctggg ctatccgatc gggtcggttc
     7561 cggcccccca cgcggacatc gtgcgctact tcgaatacct cgaccagctc ccgaccgcgg
     7621 agctccacac ctacgcccag tcctacgaag gacgcacgct cgtgtacttg gtgatcgcct
     7681 ccgagcagaa cgcggcgcgc ctctccgaga tcaaggccga ctgtcaaaag ctcgccgacc
     7741 cgcgcacctt gggcggtgcc aagccgagcg acatcgtcgc gcgcacgccc gcggtcgcgt
     7801 ggatggcgta cggcatccac ggcgacgagc tttcgagctg cgacgccgcg ctcgagctcg
     7861 cgtatcaact gaccgccgga accgacgccg ccacccgtcg catcctggac aactgcgtgg
     7921 tcgtcatcga cccgaccgag aaccccgacg ggcgcacgcg ctggctcacc cagctcgcgc
     7981 agtggaacgg cgtcatcacg agccacgacg tgcagagcat gagtcatacg ggactctggc
     8041 cctatggacg caccaaccac tatctcttcg acctgaaccg cgattggttc gcgctcgtgc
     8101 acccggagac gcgcgggcgc acggcggcca ttctcgactg gatgccgcac tatctgttgg
     8161 actgccacga gatgggcccg ctcgacacgt atctgttctc gccgccgcgc gagcccttca
     8221 acccgcacat ggtgggctac atccacaagt ggtgggagaa ggtggcgaag gagcacgccg
     8281 cgcagttcga ccggttcgga tggagctact acacgcgcga gtggaacgag gagctctacc
     8341 cgggctacgg cagctcgtgg gcgatttatc tgggcgcggt cgggttcctg ttcgagcagg
     8401 ccggcgtcga cgggtcgcag atcaagcgcc ccgacggcac cgtgatgacg taccgcgaaa
     8461 ccgtccacca ccagttcgtg ggctcgatgg cgaatctcac cgccgtggcc gacgggcgcg
     8521 ccgagttgtt gaacgattac caccgccaaa aagtcgacaa cctgcgcgcc aaaccgcgca
     8581 ccttcgtgtt cgccgccggc ggcaatcgca cccgtctcga ccgcttcgtc gaaaagctgc
     8641 agcatcagca catcgaggtc gagcgcacca cccaagggat gaaactctcc cgcgcgcgcg
     8701 cgtgggacgg caccgaggcg cgcgatgtca ccattccggc ggggagcgcc ttggttcgca
     8761 ccaaccagcc catgaagcaa ctggccgagg ccatcctcga gttcgacatc cgcattccga
     8821 cgtcgtttct gaagaccgag aagaaggaca tcctggcgcg gggcgactcg cgcttgtacg
     8881 acaccaccgg ctggtcgctc ccgctggcgt acggactgga cgcgtacgtc gtcgacggcg
     8941 cgccgcgcgt ggacagcgag gcgtacgcga tcgccgcgcc cgcgggatcg gtttccaacg
     9001 acgccgccaa ggtcggcttc gccttcgacg gccgcgacga ccgcgcgctc gaattggtgg
     9061 cgcggctctt cgagaaccaa atcagggtgt ggtgcgcgca gaaaccgttc cgcgccggcg
     9121 agacggtgat gccgcgcggg agcttcctcg tgcggcgcgc cggcaacccc ggcctcgacg
     9181 tcacgaagct gcgcgcgctc gcggaagcgg cgggcgtcgt tgtgatcggc gtcgacgggg
     9241 gcctcgcgtc gggtgcctac gccgacctcg gcggcaacga gttctcgctc ctcgtggcac
     9301 cgaggatcgc gctggtcgcg ggcaccggca ccaactcgac cagcttcggg gcgacctggc
     9361 acctgctcga cagccgcttc cgcgtgcgta cctcgatcgt cgacgtgacc cgcgtcgcgg
     9421 acaccgacct atccaagtac aacgtgatcg ttctccccga ttattccggc ggggtcgagg
     9481 cctaccgggg cgtgctgcgc aaatccggta tcgagaatct caagacctgg gtcgaggcgg
     9541 gcggcacgct ggtcgccgac ggcgcgggcg cggccatgct cgccgacacg tcggtcgcga
     9601 tcgcgtcggt gcggatgcgc gatcaggtgc tcaaggacct gcccgactac gtggcgtcgc
     9661 tcgcgctcgc caagagcgct ttttcgcccg tagtcgacag cctgtcactg tggggacccg
     9721 gcgcagccaa gaaacccgcc caggccgaag agaagaaaga cgaaaaggaa tccggcgacc
     9781 tcgaatcgcg taagcgcgag gacgaggtcg cgagaaagct cgcgccgcgc ggcgccatcg
     9841 tcgcggtcga tctcgacgag gagcactggc tggcgttcgg ctgccccaag acgattccgg
     9901 ccttgctcaa caccaatcgc accttcgtca ccaaagcgac gcaggtgccg gcgcgattgg
     9961 cgcccgcgga tcgcctgcgc ttgtccggcc tgttctggga ggaagcgcgc gcgcgctggt
    10021 ccgagagcgt gtacgcggcg cgcgacgcgg tcgggagcgg acaggtgatc gtgttcgttt
    10081 cgaccccgaa cttccgcggc tacttccacg gcgccgagcg tctactattg aatgcactgt
    10141 tgctgggtcc gggctgcggt acctcgcagc gcgtcgagtg gtagcgaaac cctcgggggc
    10201 cgattgatcg atggcgacgg tggctgcacg agtggtataa tggtgtcgct cccctgaagt
    10261 acccgtgacc gcccccgtcg tatgagcgct ttcaaccgtc gcaatggcat cgtcgctctg
    10321 gccgttgccg ccgtcgcggc cgcggcgctc gcgtcggctg ccgacccgcc caccgccacc
    10381 ggtggcgccc cgccggatgt tcgccgcatc gaaaagttcg tccacatcgc cgcgtttctg
    10441 aacctcgcgc aaggaaccgt gcgcatggtc gcggtacttc ccatcgacgg ggagtcgtcg
    10501 gccgcggtgc tcgacaccgt cgcggcggtc atcaggggca acccgagcaa gcgcctgcgc
    10561 gcctacgtcg tgctgcgcgg ttcggattcg tcgctgcgcg ccgccgtcct ggccggccgc
    10621 gccgccgatc cgcgcatcgt gttcttctgg gacccggccg gtacggccac cgcggcctgg
    10681 cgagccgacc cggcgcgtgc gggcgtgtgg ctttacgaca cctcggcgcg cttcaccggc
    10741 gggccgccgg gagcgtcgct cgtcgtgggc gcacccgccg ccacccccga agcgcccctg
    10801 gcagggccgg cgttgcgcga gcaagccggc gagttggtgc gccgggtgga agcgaagatg
    10861 gcgcaggcgg acgacggatc gtgaccattc cctatccacc ggtatggatt gaccgggccg
    10921 cgtctcgcgg ataacgttcg gccatgcaca cccaatcgaa gcccgtgttc cgtgagattc
    10981 tcgtggccgg agtgctggtg gcggctctcg ctccggcggc ctgggccgcg cgcggcgaca
    11041 tcacgctggt cgggcagatc aagccgaccg ggagcgatca gttctgcaac gtgtgggggt
    11101 tcgagcgcga cggccgtgac tacgccgtgg tcggggactg ggacagcgga cccatcatca
    11161 tcgacgtcac cgaccccacc aatccgttcg tgcgcaaggt gatcacgggc accggcgtgt
    11221 tcggcttcga cgtcaaggtg tggggaaact acatctattg ttgcgagggc ggcttcggtc
    11281 acacgggcgc atcgagccgc gccatcgaca tcaccgattt cgacaacccg atcatctcgc
    11341 ccgcgttcac caacgcgcac gcgctcgcga ttcatcccga cgggtatctg ttcgcggaga
    11401 tcccgggcct gcgttgctac gatctcaacg ccgaccccac gcccgcgggc tacctctggt
    11461 acgacggcac gtccgggggc cacgattcca ccgtcgacgt cgaacacgac cgcctgtacg
    11521 acttccacgg ctacgacggg gtgacgcgca tctgggacat ctcgaacatc aacagccggc
    11581 agttgctggg ttcgattacc gacccgacca tcaattatca ccacagcggc gatgcgaccg
    11641 aagacggcaa ttacctctac gtgtgcgacg agctcgcgac cgggctcgag aaagacctca
    11701 cggtgtggct gatctccaac ccggcggctc cggtgaaagt gggcagcctg ttcgaccccg
    11761 tgtcgacgat ccacaacctc tacatcatcg ggaacttcgc gttcgtgtcc cactactcgg
    11821 ccggcttccg ggtctacgac gtgtcgaatc cggccgcccc cgagctgctc gacacctacg
    11881 acaccgcccc cctgcgcaac ggcgacggat acaacggatg ctacggcgtc tacccgttcg
    11941 ggccgggcgg tatcgtgtac gcgtccgata gcgacaacgg gctctatctc ttcgcggtcg
    12001 aggacttcgc cggcgtgccg tccagtgtcg tcgcgacgcc gggcgccaag acggcgcgcc
    12061 tcgtcggcaa ttaccccaac cccttcaacc cctcaaccac gatcgcgtac gaagtcgcgc
    12121 gcgagggcga cgtatcgctc gcggtgtacg acgtggacgg agccctcgtg cgcgtcctgg
    12181 cccgcggccg cgcgcccgcg ggtcgccacg agacgcgctg ggacggcgcc gacggcggcg
    12241 gccggcgcgt cgcttcgggg gtctatttcg tgcgactcgc agccaacggg gccgtcgaca
    12301 cgggacgcct ggtattgctc aagtgaagat cgatgcggta gagtcgtcgc atcgatgaag
    12361 ccgctgtccg cccatactcc gcgcgccgcg gcgaccatgg ccgccggctt cgcgatggtc
    12421 gcggggaccg ccgcgtccgc ggccgtcgac gtccagctgg tcggccgcac caacctcccc
    12481 aacagcatct ttctggtcaa cgtggtgggc tacgtcgatc agacgacgct gcgcgagtat
    12541 gcgatcgtcg gcgacaacca cgacaaggtg tacatcgtcg acgtgaccga tccggccgac
    12601 atgcgcatca ccgcgcagat cgcgggtatt cccggcttcg acgtgaagac gtggggccag
    12661 tacctgtaca cgtgcgacgg caacacgggc ggcaacgaca gccgcatcgt cgacatcctc
    12721 gacccggcca acccgatcgt cttgcccgac gggtttgctt ccgcgcacac gttccagatt
    12781 tcgtcggcgg gaatcctgtt cgccgagttc ccaggcctgc gcatttacga cctcaacacg
    12841 acgccgaccg cgccccaact ccgccacgaa tccggc
//
LOCUS       DASPGK010000360         7959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_438825, whole genome shotgun sequence.
ACCESSION   DASPGK010000360 DASPGK010000000
VERSION     DASPGK010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438825"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1388
                     /locus_tag="VEC56_10025"
     CDS             186..1388
                     /locus_tag="VEC56_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HXV14529.1"
                     /translation="MSKVLCVLYDDPVDGYPTSYAIGTLPKLDRYPGGQSMPSPKQID
                     FKPGELLGSVSGELGLRKFLEANGHTLVVTSDKDGADSTFDRELSDAEIVISQPFWPA
                     YLTAARIAKAPKLKLAITAGIGSDHVDLQAAMDRGITVAEVTYCNSISVAEHVVMMIL
                     SLVRNYIPSYDWVIKGGWNIADCVARSYDIEGMSVGTVGAGRIGLAVLRRLKPFDVRL
                     HYTDRHRLPAAVEQELNLVFHPSVESMVRICDVVTINTPLHPETEHMFNDALIGKMKR
                     GAYLVNTARGKICDRDAVARALRDGQLAGYAGDVWFPQPAPKDHPWRSMPHHGMTPHV
                     SGTSLSAQARYAAGVREILECWFDRKAIRNEYLIVKDGKLAGAGAHSYSAGNATGGSE
                     EAAKFKKK"
     gene            1396..1878
                     /locus_tag="VEC56_10030"
     CDS             1396..1878
                     /locus_tag="VEC56_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV14530.1"
                     /translation="MPGPEIDVLLEMIDQAFERRAWHGTNLRGALRGVTAKEAAWRPG
                     RGRHNVWELVVHAAYWKYAVRRRLLGEKRGSFPLEGSNWFARPERGKATEAAWKADIA
                     LLVQTHKSLRAAVAALSSRDLGARPRGTRFDTRGLVVGIAAHDLYHTGQIQLVKRLMP
                     "
     gene            1974..2597
                     /locus_tag="VEC56_10035"
     CDS             1974..2597
                     /locus_tag="VEC56_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXV14531.1"
                     /translation="MAIKLFPKEINFFDIFEKSSQNLIDASTQLVALFERFDDLDARI
                     KRIHDIEHDNDELTHEIMSRLSQTFLTPIDREDIHALATSLDDVLDFLWAAGDRVQLF
                     GVASTRPGAIKLARSLEASCEVVRHAFQELKRKKYPSVLKDCIEINRLENDADLIFRE
                     AIGELFEHERDPIVIIKWKEILEHIELATDRCEDVANTLESVVLKHA"
     gene            2590..3576
                     /locus_tag="VEC56_10040"
     CDS             2590..3576
                     /locus_tag="VEC56_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXV14532.1"
                     /translation="MLEIVIVTVVLAVLFDVSNGWNDSANSIATVVSTRVLSPQRAVM
                     LAAVMNVLGALVSTEVAKTIGTGVVDPSAVSQVVVASALVTGFLWNGAMTVLGLPVSA
                     SHALIGSLIGAAGAYAGWGVLNDAGIKKILLALLISPVLGLAIGYVLMRLILLVFGRY
                     SPWAINRNFGRLQLVSSAFMAFSHGSNDAQKVMGIITLSLFSGGLIESIEVPTWVVLM
                     CAVAMGLGTALGGWKVIRTLGMKMLRLEPVHGFAAETAATAIILGASHAGLPVSTTHV
                     ITSTIMGVGSTKRLSAVRWGVAWKIVLAWVFTLPACIAFAYVIQRFLFSRIF"
     gene            3602..5248
                     /locus_tag="VEC56_10045"
     CDS             3602..5248
                     /locus_tag="VEC56_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14533.1"
                     /translation="MNRVVVLLSFLSIVAAPVRAQDDSHAACAAPPSYVPVDLLEREV
                     GLRKGVGNSRETVATTSKDAQAFYNQGLNYLESYVWIEASRSFHQALRLDPDLAMAYV
                     GLSRTHSGLDDPAGAKLYFEKAKALAPKASDRERRRIAIREAQLAAMENIEDTAKLQA
                     YRKVVDDALAIDIDDPQLWLLAGNAQEANAAGRGQRGTAATVAYYDQVLRLVPNHASA
                     HHYLVHSYETIGLIDKALEHGEVFARMAPSIPHAAHMWGHDLRRVGRIDDAIVEFKKA
                     DALENAYYKAENIDPSFDWHHTHNLDLLATCYQHKGQMSLAESTMREAVALGGRDAYR
                     AFNTRILPAFLIHRARYDEALEAARAMLELPYAQSRTVGYALAAQALIGLGRIPEARL
                     ELMSAESELEKVPVVTPGIVPRRSMVEGWVVAARGELLLETGQTDEAGRILKEAQQAL
                     RAAPGPDAWTQALFRLETMARSAREGGDWDLAEYTAQQMLDHDAAYGGSHLAMALVLD
                     HKGDAAGAKRELEAARKYWRDADRDLPELKQIATALASAR"
     gene            complement(5258..5812)
                     /locus_tag="VEC56_10050"
     CDS             complement(5258..5812)
                     /locus_tag="VEC56_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14534.1"
                     /translation="MTWKRGILFLIAVLLWAGCGDDDSTGSGEPVNPVDINGALVIPS
                     AWAGTWEITLTFRDCATDQIRSQEVITSLACPGDTLVNPFVAIFEDCDGTRTGNHLEA
                     SCHYQNASGACQITMDLDFTMDVNGNSLSGSGTIQTTATPECGTFFTAGCEEVVISGT
                     RLSTDTSTCPNIAVTHPARRAFLR"
     gene            complement(5809..6528)
                     /locus_tag="VEC56_10055"
     CDS             complement(5809..6528)
                     /locus_tag="VEC56_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14535.1"
                     /translation="MKLPNRHRFATLTALAVLLAGMTTGCLTLCEALLGIEPEPGKDF
                     VVVVPAGEQATLADALYWLSRITLCLDEIAAEGGGIAPTGVYCRTSRDFRGSEPTATA
                     ELVTSASTAPGRYFIPYRYADPTTFITGTVDLTVEENQSGVVAELYITSKTEVPVVNE
                     PVITYGCLSTSPASDPIVEYRWWFNYNGNPSSTPSEVTTICTNSTVYTTPGTKTVRLV
                     VRTQSGSEAEDIQTFTVLGAR"
     gene            complement(6633..>7959)
                     /locus_tag="VEC56_10060"
     CDS             complement(6633..>7959)
                     /locus_tag="VEC56_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14536.1"
                     /translation="GFTFVTTTDFFTGSSACIDRVARTATLNVASLGTDPVARHFNGK
                     VYVVNRSVASNIQILNPAAGFATIDQWSTGNGSGPHDIYVRPDGVTAYITRYDMTTLL
                     KMNLTTGATLATINLSAFADADGIPEMDQMFWQNDMLFVTVQRLDRNNFYAPTGTSYL
                     VKIDMTTDTVVDMDPGTLGVQAVPLVRTNPYSEIVDKLSGGKVWFSAVGFFGVNDGGI
                     IETGAADPHTQTVILTEAAAGGDILDVARFTLDKSFAIVANASFQTELIAFNPQTGAK
                     IGPTIYNPGGYDLSDIEVGLDLLVADRKATNPGIRFFDPSTHAQQTVNPVSTGLPPFD
                     VLEQVELPVGVGDTPPAAALGRNYPNPFNPDTSIPFSLARAGRATLTIYDVNGRFVAM
                     LLDEHRGAGEHVARWDGRDAAGRASSSGVYFARLQANGIVATHKLVLLK"
BASE COUNT         1386 a         2500 c         2707 g         1366 t
ORIGIN      
        1 gcccgcgggg acacaggaga agtttcggga gctcaagcat ctggagaagt cgatcggccc
       61 caccgggcgc ctcgcgattg cgcccttcct gcacacgagc tcgcgcgatt tgtggcaaat
      121 ctacatgctg gaccagtgaa ggtgtatact cccggcttcg accatatcga gaggaacggt
      181 gagccatgag taaagtactg tgcgttttgt acgacgatcc cgtggacgga tacccgacga
      241 gctacgccat cgggaccctc cccaagctcg atcgctatcc gggcgggcaa tcgatgccct
      301 cgccgaagca gatcgacttc aagccgggcg agctgctcgg ttccgtctcc ggcgagctgg
      361 ggctgcgcaa gttcctggag gcgaacggac acacgctggt cgtgacgtcc gacaaggatg
      421 gcgccgactc gaccttcgat cgggagctct ccgacgcgga gatcgtgatc tcgcagccgt
      481 tctggcccgc gtacctgacc gcggcgcgca tcgccaaggc gcccaagttg aagctcgcca
      541 tcacggccgg catcggctcc gaccacgtcg acctgcaggc cgcgatggac cgcggcatca
      601 cggtcgcgga agtgacgtac tgcaacagca tcagtgtggc cgagcacgtc gtgatgatga
      661 ttctatcgct cgtgcgcaac tatattccgt cgtacgactg ggtgatcaag ggcggctgga
      721 acatcgcgga ctgcgtggcg cgctcgtacg atatcgaagg catgtcggtg gggacggtgg
      781 gcgcggggcg catcgggctg gccgtgctgc gccggctcaa gcccttcgac gttcggcttc
      841 attacacgga ccgccaccgg ctcccggccg cggtcgagca ggagctcaac ctcgtgttcc
      901 atcccagcgt cgagtcgatg gtgcgcatct gcgacgtcgt gaccatcaac acgccgctcc
      961 accccgagac cgagcatatg ttcaacgacg cgctcatcgg caagatgaag cgcggtgcgt
     1021 acctggtgaa cacggcgcgc ggcaagatct gcgaccgcga cgcggtcgcg cgcgcgctgc
     1081 gcgacggcca gctcgcgggc tacgccggcg acgtgtggtt cccgcagcca gcccccaaag
     1141 accacccgtg gcgatcgatg ccgcaccacg gcatgacgcc gcacgtgtcg gggacgagcc
     1201 tttccgcaca ggcgcgctac gcggccggag tgcgcgagat tctcgagtgc tggttcgacc
     1261 gcaaagcgat tcgcaacgag tatctgatcg tcaaggacgg caagctcgcg ggagccggcg
     1321 cgcactcgta cagcgcgggc aacgcgaccg gcggctccga ggaagcggcg aagttcaaga
     1381 agaagtaggt gcgcgatgcc gggcccggaa atcgacgttt tgctggagat gatcgaccag
     1441 gccttcgagc gccgtgcgtg gcacgggacc aacttgaggg gtgccctacg gggcgtgacc
     1501 gcgaaggaag cggcgtggcg gccgggacgc ggtcgccaca acgtgtggga gctcgtcgtt
     1561 cacgccgcgt actggaagta cgccgtgcgg cggcggctcc tgggagagaa gcgcggctcg
     1621 tttccgctcg agggatcgaa ctggttcgcg cggccggagc ggggcaaggc caccgaagcg
     1681 gcgtggaagg cggacatcgc acttctcgta caaacgcaca agagcctgcg cgccgcggtg
     1741 gcggcgctct cgagccgcga cctgggtgct cgaccgcgcg ggacccggtt cgacacccgc
     1801 ggcctggtgg tcggtatcgc cgcgcacgat ctctatcaca ccggtcagat tcaattggtc
     1861 aagcgactga tgccgtagtc ccggcgcggt tttccagcac gcgttgccat ttctttacgg
     1921 attctttatg atgcccgttc ggtgccgcga gacggatcca ccccggaggg cccatggcga
     1981 tcaaactgtt tcccaaagaa atcaacttct tcgacatctt cgagaagtcg tcgcagaacc
     2041 tgatcgacgc gtcgacccag ctcgtcgcgc tattcgaacg cttcgacgac ttggacgccc
     2101 gcatcaagcg gatccacgac atcgagcacg acaacgacga actcacgcac gagatcatga
     2161 gccggctgag ccagacgttt ctgacgccga tcgatcgcga ggacatccac gcgctcgcca
     2221 cctcgctcga cgacgtcctc gacttcctgt gggccgcggg tgaccgcgtc cagctgttcg
     2281 gcgtcgcctc cacccggccg ggcgcgatca agctggccag gtcgctggag gccagctgcg
     2341 aggtggttcg gcacgcgttt caggagctca agcgcaagaa gtacccgtcc gtgctcaagg
     2401 actgcatcga gatcaaccgg ctggagaacg acgcggacct gattttccgc gaggccatcg
     2461 gcgagctgtt cgagcacgag cgcgatccca tcgtcatcat caagtggaag gagatcctgg
     2521 agcacatcga attggccacc gaccgttgcg aggacgtcgc caacacgctc gagtccgtcg
     2581 ttctaaagca tgcttgagat cgtcatcgta acggtcgtgc tggcggtcct cttcgacgtc
     2641 agcaacggct ggaacgactc cgccaattcg atcgctaccg tggtatcgac gcgcgtgctc
     2701 tcgccgcagc gtgccgtgat gctcgccgcg gtgatgaatg tgctgggcgc gctggtatcg
     2761 accgaggtgg cgaagaccat cggcaccgga gtggtggatc cgtccgcggt gagtcaggtg
     2821 gtggtggcga gcgcgctggt taccggcttc ttgtggaacg gcgcgatgac ggtcctcggg
     2881 cttccggtga gtgcctcgca cgcgctgatt ggcagtctga tcggggcggc aggtgcgtac
     2941 gcgggatggg gcgttctcaa cgacgccggc atcaagaaga tccttctggc gctgctcatt
     3001 tcgcccgttc taggcctcgc gatcggttac gtgctgatgc ggctcatcct gctggtcttc
     3061 gggcgctact cgccgtgggc catcaatcga aacttcggcc gcttgcagtt ggtctcctcc
     3121 gcgttcatgg cgtttagcca cggctccaac gacgcgcaga aggtgatggg catcatcacg
     3181 ctctcgctgt tcagcggcgg gctgatcgaa tcgatcgaag tgcccacctg ggtggtcttg
     3241 atgtgcgcgg tcgcgatggg cctgggtacc gcgctcggcg gatggaaggt gatccgcacg
     3301 ctgggaatga agatgctgcg gctcgagccg gtgcacggct tcgccgccga gacggccgcc
     3361 accgcgatca tcctgggagc gagccacgcc ggcttgccgg tgagcacgac gcacgtgatc
     3421 acgtcgacta tcatgggcgt gggatcgacc aagcggctct cggccgtccg ctggggcgtg
     3481 gcgtggaaga tcgtgctggc gtgggtcttc acgctcccgg cctgcatcgc gttcgcctac
     3541 gtgatccagc gttttctctt ttcgaggatc ttctagcgag tacttccagg agcgtgcaac
     3601 catgaaccga gtcgtcgtcc ttctgtcttt cctctcgatc gttgcagcac cggtacgggc
     3661 ccaagacgac tcccacgccg cgtgcgcggc gccgcccagc tacgtccctg tcgacctcct
     3721 cgagcgcgag gtcggtctgc gcaagggggt tggcaactcg cgcgagacgg tggcgacgac
     3781 gtccaaggac gcccaggcct tttacaacca gggcctcaac tacctcgagt cgtacgtatg
     3841 gatcgaggcc tcgcggtcct ttcaccaggc gctgcgtctc gatcccgatc tcgccatggc
     3901 gtacgtcggc ttgagccgca cgcactccgg cttggacgat ccggcgggcg cgaaactcta
     3961 tttcgaaaag gcgaaagcgc tcgcacccaa ggccagcgac cgcgaacgcc ggcgtatcgc
     4021 gatccgcgag gcgcagctcg cggccatgga gaacatcgaa gacacggcga agctccaggc
     4081 gtatcgcaag gtcgtcgacg acgccctcgc gatcgacatc gacgatccgc agttgtggct
     4141 gctggcgggc aacgcgcagg aggccaacgc ggcgggacgc gggcagcgcg gcaccgcggc
     4201 caccgtcgcg tattacgacc aagtcctccg gctcgttccc aaccatgcga gcgcgcacca
     4261 ctacctcgtt cattcgtacg aaacgatcgg gctgatcgac aaggcgctcg agcacggcga
     4321 agtgttcgcg cgcatggcgc cgtcgattcc gcacgcggcc cacatgtggg ggcacgacct
     4381 gcggcgcgtg ggtcgcatcg acgacgccat cgtcgagttc aagaaggccg acgcgctgga
     4441 gaacgcatat tacaaggccg agaacatcga tccgtccttc gactggcacc acacgcacaa
     4501 cctcgacctg ctcgcgacct gctaccagca caagggacaa atgagcctcg cggagtcgac
     4561 catgcgcgaa gccgtcgcac tgggcggccg cgacgcctac cgcgcgttca acacgcgcat
     4621 cctgcccgcc ttcttgatcc atcgcgcgcg ctacgacgag gcgctggaag cggcgcgcgc
     4681 catgctggag ctcccgtatg cgcagtcgcg cacggtgggg tacgcgctcg cggcccaggc
     4741 gctgatcggg ctggggcgca ttccggaagc tcgactcgag ctcatgtcgg cggagagtga
     4801 attggagaag gtcccggtcg tgacgccggg catcgtgccc agacgctcga tggtcgaggg
     4861 ctgggtcgtc gcggcgcgcg gcgaattgct cctcgagacc ggccagaccg acgaagccgg
     4921 gcgcattctc aaggaggcgc agcaggccct gcgtgccgcg ccgggccccg acgcgtggac
     4981 acaggcgttg tttcgtctcg agacgatggc gcgcagcgcg cgcgaggggg gagactggga
     5041 cctggcggaa tacaccgcgc agcaaatgct cgaccacgac gcggcctacg gcggcagcca
     5101 tctcgcgatg gcgctcgtgc tcgatcacaa aggcgacgcg gcgggcgcga agcgcgagct
     5161 ggaggcggct cggaagtact ggcgcgacgc ggaccgcgat cttccggaac tgaagcaaat
     5221 cgcgacggcg ctggcgtcgg cgaggtaggg gcgatcgcta gcgcaggaac gcgcgccggg
     5281 cggggtgcgt cacggcaatg ttcggacacg tcgaggtgtc ggtgctcaag cgggtgccgg
     5341 agatgacgac ctcttcgcac ccggcggtga agaacgtccc gcactcgggg gtggcggtgg
     5401 tctggatggt gccgctaccc gagagcgagt tgccgttgac gtccatcgtg aagtccaggt
     5461 ccatcgtgat ctggcaggcg ccgctcgcgt tctggtagtg gcagctcgcc tcgaggtggt
     5521 tgccggtgcg ggtgccgtcg cagtcctcga agatggcgac gaacgggttg accagcgtgt
     5581 cgcccggaca cgcgagcgac gtgatcactt cctgcgagcg aatctggtcg gtcgcgcagt
     5641 cgcggaaggt cagcgtaatc tcccaggtcc ccgcccacgc ggaggggatc acgagggcgc
     5701 cgttgatatc gaccggattg accggttcgc cgcttccggt cgagtcgtcg tcgccgcagc
     5761 cggcccacaa gagcaccgcg atcagaaaga gtatgccgcg cttccaggtc atcgtgcacc
     5821 cagtacggta aaggtttgga tgtcttcggc ctcgctgccg ctctgcgtgc gaaccaccaa
     5881 gcggaccgtc ttcgtgcccg gcgtcgtgta caccgtgctg ttcgtgcaaa tggtcgtgac
     5941 ctccgagggc gtcgacgacg gattgccgtt gtaattgaac caccagcggt actcaacgat
     6001 cggatcgctc gcgggcgacg tgctcaagca tccgtaggtg atcacgggct cgttcaccac
     6061 cgggacctcg gtcttggagg tgatgtacaa ctcggcgacg accccgctct ggttctcttc
     6121 gacggtcagg tcgacggtgc ccgtgatgaa agtggtcgga tcggcgtagc ggtagggaat
     6181 gaaatagcgc cccggcgccg tcgacgcgga ggtgacgagt tccgccgtgg cggtcggctc
     6241 gctcccacgg aaatcgcgcg aggtccggca gtacacgccc gtgggggcga tgcccccgcc
     6301 ctcggcggcg atctcgtcca ggcacaacgt gattcgggag agccaataga gtgcgtccgc
     6361 cagcgtcgcc tgctcgccgg ccggaaccac gacgacgaag tccttgccgg gctcgggctc
     6421 gatccccagc aaggcctcgc acagggtgag gcatccggtg gtcattccgg ccaggaggac
     6481 cgcgagggcg gtcagggtgg cgaatcgatg gcggttcggg agcttcatgg ttccgtccta
     6541 cccttcggac acacgaagtc ccgaaccgtc acatggaaat tttacggatc gtctcgaccc
     6601 cacagggcga ccacgtccgc gtcggggtcc actcacttga gcagcacgag cttatgggtc
     6661 gcaacgatcc cattcgcctg aagccgcgcg aagtagacgc ccgatgacga cgcgcgcccg
     6721 gccgcgtcgc ggccatccca acgcgcgacg tgctcgcccg cgccgcgatg ctcgtcgaga
     6781 agcatggcca cgaagcgccc gttcacgtcg tagatcgtga gagtcgcgcg acccgcacgc
     6841 gcgagcgaga aggggatcga cgtgtcgggg ttgaagggat tggggtagtt ccggccgagt
     6901 gcggcggcgg ggggcgtgtc gcccacaccc acggggagct cgacttgctc gaggacgtcg
     6961 aagggcggca accccgtgct gacggggttg accgtctgct gcgcgtgcgt cgaggggtcg
     7021 aagaagcgaa tgccggggtt ggtcgccttg cgatccgcca ccaagaggtc gagaccgact
     7081 tcgatgtcgc tcaagtcgta gccgcccggg ttgtagatgg tcggaccgat ttttgcgccg
     7141 gtttgcgggt tgaacgcgat caattcggtt tgaaacgacg cgttggcgac gatcgcgaac
     7201 gacttgtcca gcgtgaagcg cgccacatcc aggatgtcgc cgcccgcggc ggcctcggtc
     7261 aggatgacgg tctgggtgtg gggatcggcg gcgccggttt cgatgatgcc gccgtcgttg
     7321 acgccgaaga atcccaccgc cgaaaaccac accttgccgc ccgacagctt gtcgacgatc
     7381 tcggagtacg ggttggtgcg aacgagcggc acggcctgca cccccaacgt gccagggtcc
     7441 atgtccacca cggtgtcggt ggtcatgtcg atcttgacca ggtagctcgt gccggtgggg
     7501 gcgtagaagt tgttgcgatc gagtcgctgc acggtgacga agagcatgtc gttctgccag
     7561 aacatctgat ccatctcggg gatcccgtcg gcatccgcga aggcggagag gttgatggtc
     7621 gccagcgtgg cgccggtggt gagattcatc ttgagcaatg tggtcatgtc gtagcgcgtg
     7681 atgtacgcgg tgacaccgtc ggggcggacg tagatgtcat gggggccgga gccgttaccg
     7741 gtggaccact gatcgatggt ggcgaacccg gcggccgggt tcaggatttg gatgttgctc
     7801 gccacactac gattgacgac gtagaccttg ccgttgaagt gacgcgccac cgggtccgtg
     7861 cccagcgaag cgacgttgag ggtcgccgtc cgcgcgacgc ggtcgatgca ggccgacgat
     7921 ccggtgaaga agtccgtggt ggtgacgaag gtgaagcct
//
LOCUS       DASPGK010000361         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1023352, whole genome shotgun sequence.
ACCESSION   DASPGK010000361 DASPGK010000000
VERSION     DASPGK010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023352"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="VEC56_10065"
     CDS             <1..1709
                     /locus_tag="VEC56_10065"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HXV14537.1"
                     /translation="ASVAQKRSVAMVTTHELAHMWFGDLVTMSWWDDLWLNESFADWL
                     AMKLTHELFPEYRLDTHELGEVNQVMLSDARPSTTTVRRKVDSGADIMEDLGLAYEKG
                     RTVLRMTEEFIGPDAFQRGVRDYLERHKWGNAVAADLFSALSQAADENLQPMLQSFLE
                     QPGYPLVKVDVNPNGLVTLSQRRFLNAGAKADEHTWTTPVRLKISDGKNVQTRVVVLD
                     KASTSVEVNGHVDWVMPDAGGVGYYRWVVPADMMMKLAENPDKTLDDRERARFLGNAR
                     ALLNAGEISGDEYLALAAAFSKHPDPQIVAALIADLGSLKMPFVTDDLAEPFAGYVRR
                     TLGPAREEFGIEPRADDIEAVKLLRPGLITWLGDDGRDPEVRVFCKDLAKRYMSDPSS
                     IDATIAGAVLGVAASDGTREDFLAYQQKFETSKVPADRSRYLSALGKFDDPKLQDEVL
                     EYALTDKVRPTEMYQVVSGMFDTEAGRDRIYAWMTKNYDRLTGRMPPEFVAYLPYFVT
                     GCSEQRLEAARKFFASPSHNVDGTDANLAKVSDTIGDCLNLREREGKAVAGYLRTAAS
                     AP"
     gene            1706..>2958
                     /locus_tag="VEC56_10070"
     CDS             1706..>2958
                     /locus_tag="VEC56_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV14538.1"
                     /translation="MTPARALRVAIVGSGPSGFYAAESLLAATAPGVEVDMFEALPAP
                     FGLVRYGVAPDHPKIKTIIARYEKTAEHPSFAFLGNAEVGRDITVDELRRYYDAIVFA
                     SGAQTDRRLGIPGEDLPGSYTATEFVAWYNGHPAYQDRVFDLSCECAVVVGQGNVAVD
                     VARILAKTVDELAGTDITARALEVLAESKIREVRMVGRRGPVQAAFTPVELKELGELV
                     DCDVVVDPAELVLNDASRAELDVLKWRDAKKNVDLLNGFAAQAALGRARRLVIEFYRS
                     PKEIRGREAVGEILVERNALTGEAGAQRAVGTGNTETVACGAVFRSVGYRGVPIAGLP
                     FDEKAGIVPNVEGRVRRDGVPVPGLYVVGWIKRGPSGVIGTNKPDAVETVKHLLADAP
                     ALAPCAEPSRDAVLSLLESRRVRVV"
BASE COUNT          502 a          969 c         1030 g          457 t
ORIGIN      
        1 ccgccagcgt ggcgcagaag cgtagcgtgg ccatggtgac cacgcacgag ctcgcgcaca
       61 tgtggttcgg cgacttggtg acgatgtcgt ggtgggacga cctgtggctc aacgagtcgt
      121 ttgccgactg gctggccatg aagctcacgc acgaattgtt ccccgagtac cgcctggaca
      181 cgcacgagct gggcgaagtc aatcaggtaa tgttgagcga cgcgcgcccg tcgacgacga
      241 cggtgcggcg caaggtcgac tcgggcgcgg acatcatgga agacctgggc ctcgcgtacg
      301 agaagggccg caccgtgctg cgcatgaccg aggagttcat cggaccggac gcgttccagc
      361 gtggcgtgcg cgactacctc gagcgccaca agtggggcaa tgcggtggcg gccgacctgt
      421 tctccgcgct gtcgcaggcg gccgacgaga acctgcagcc catgcttcag agcttcctcg
      481 agcagcccgg ctatccgctg gtgaaggtgg acgtcaatcc caacgggctc gtgacgcttt
      541 cgcagcggcg cttcctgaac gcgggggcca aggccgacga gcacacctgg acgacgccgg
      601 ttcgactcaa gatctcggac ggcaagaacg tgcagacgcg cgtcgtggtg ctggacaagg
      661 cgtcgacgag cgtcgaagtc aacggacacg tcgactgggt gatgccggac gcgggcgggg
      721 tgggctatta ccgctgggtc gtgccggccg acatgatgat gaagctggcc gaaaaccccg
      781 acaagacgct ggacgatcgc gagcgcgccc gattcctggg caacgcacgc gcgctgctca
      841 acgcgggcga gatctccggc gacgagtacc tcgcgttggc cgccgccttc tcgaaacacc
      901 ccgacccgca gatcgtggcc gcactgatcg ccgacctcgg gagcctcaag atgcccttcg
      961 tcaccgacga cttggcggag cccttcgcgg gctacgtgcg ccgcacgctg ggccccgcgc
     1021 gcgaggagtt cgggatcgag ccgcgcgccg acgacatcga agcggtgaag ctgttgcgtc
     1081 ccggcttgat cacctggctg ggcgacgacg gacgcgaccc cgaggtgcgc gtattctgca
     1141 aggacctcgc gaagcgctac atgagcgatc cctcgtcgat cgacgccacc atcgcgggcg
     1201 cggtgctggg tgtcgcggcc agcgacggca cgcgcgagga ttttctcgcc taccagcaga
     1261 agttcgagac cagcaaagtg ccggcggatc gcagccggta tttgtcggcg ctcggcaagt
     1321 tcgacgatcc caagttgcag gacgaggtgt tggagtacgc gctcaccgac aaggtgcgcc
     1381 cgaccgagat gtatcaggtg gtctcgggca tgttcgacac cgaggccggc cgtgacagga
     1441 tctacgcgtg gatgacgaag aactacgacc ggctcaccgg acgcatgccg cccgaattcg
     1501 tcgcgtattt gccgtacttc gtgaccgggt gctcggagca gcggctggaa gcggcgcgca
     1561 agttcttcgc gtccccgtcg cacaacgtcg acggcaccga cgccaacctg gccaaggtgt
     1621 ccgacaccat cggcgactgc ttgaacctgc gcgaacgcga gggcaaggcg gtcgcggggt
     1681 atctgcgaac ggcggcgagc gcgccgtgac ccctgcccgg gccttgcgcg tcgcgatcgt
     1741 ggggagcggc ccgagcggtt tctacgccgc cgagtcgctc ctcgccgcga ccgcgcccgg
     1801 cgtcgaagtg gacatgttcg aggcgctccc cgcgcccttc ggcctggtgc gctacggtgt
     1861 cgcaccggac caccccaaga tcaagaccat catcgcccgt tacgagaaga ccgcggagca
     1921 cccgtcgttc gcgttcctcg gcaacgccga agtcggccgt gacatcacgg tcgacgagct
     1981 gcggcgctac tacgacgcga tcgtgttcgc cagcggcgcg cagaccgacc gccgtctcgg
     2041 catccccggc gaagacctgc ccggcagcta caccgcaacc gagttcgtcg cctggtacaa
     2101 cggacatccc gcgtaccaag accgcgtgtt cgacctctcg tgcgagtgcg cggtcgtggt
     2161 cggtcagggc aacgtggccg tcgacgtcgc ccgtatcctc gccaagaccg tggacgagct
     2221 ggccggcacc gacatcaccg cgcgcgccct cgaggtgctc gcggaatcca agattcgcga
     2281 ggtgcgcatg gtgggccggc gcgggcccgt gcaggctgcg ttcaccccgg tcgagttgaa
     2341 ggagttggga gaactcgtgg actgcgacgt ggtcgtcgac cccgccgagc tggtcttgaa
     2401 cgacgccagc cgcgccgagc tggacgtgct caagtggcgc gacgccaaga agaacgtcga
     2461 cttgctcaac ggcttcgccg cgcaggcggc gctggggcgt gcgcggcgtc tcgtcatcga
     2521 gttctaccga agcccgaaag agattcgcgg ccgggaggcc gtgggcgaga ttctcgtgga
     2581 gcgcaacgct ctcaccggcg aagcgggagc gcagcgcgcg gtcggcaccg gtaacacgga
     2641 gacggtcgcg tgcggggccg tctttcgaag cgtgggctat cgcggcgttc cgatcgccgg
     2701 ccttcccttc gacgaaaagg cgggtattgt ccccaacgtc gaggggcgcg tgaggcgcga
     2761 cggcgtcccc gtacccggcc tctatgtggt ggggtggatc aagcgcggac cgtcgggcgt
     2821 catcggcacc aacaagccgg acgcggtgga aaccgtgaaa catctcttgg ccgacgcgcc
     2881 cgcgctcgcg ccgtgcgcgg agccgtcgcg cgacgcggtg ctatcgctct tagaatcgcg
     2941 tcgtgtgcgg gtggtgag
//
LOCUS       DASPGK010000362         5093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1259086, whole genome shotgun sequence.
ACCESSION   DASPGK010000362 DASPGK010000000
VERSION     DASPGK010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259086"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="VEC56_10075"
     CDS             complement(<1..1994)
                     /locus_tag="VEC56_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14539.1"
                     /translation="MNTHLRLGSAGGPMGLRRRDVVAFCIWVAILVASACFAHAEEAP
                     ADTTFNPSDLDAFFADGSQRADSAAAPAPTPRDTVAAQPVVPITMAPVDTQATAAVAE
                     ASPVVAPPADFARHLVAYEEAQLRRGAVTMPVAAQVEYRQALAAIAQGDKEGAEKRLR
                     TALSLAPRYSDAYFTLARLHAARFSPDAVYYMVEGVMVQLKTFDGQRAFALNAVVGAT
                     LVLLLASAIVWIALAVRYFPFIAHRIAESARTKFNATGGRVAAALLLLAPFALLPGYT
                     SAAALILVLTWPFMHKRERVLSMVMAGLFAAFTGLSPLIDRFSIVADPGSLTSLIARA
                     NESPADDVLARAISAAPMSDPRIDDDRYTALGMLAMRDGDSETAAANFLRAISINKNS
                     AIAYVNLGNVYYQNGQFNKALEGYRKAEEVDSTDAVSQYNLAQAYIKTLLMGESSQAL
                     SRASKLGLEHARDAIAEPARARMAVYPKTYSSAQIWKIAAVEGKYHNPALVTHLLETV
                     TGHPTTVSAGIMLVAMILAVVVSKTLKSRKLAFQCANCGDLTCEGCCNDELGSVICHA
                     CGEAVAGVSSDRVLEALLRQRRQSVIVRRRKSIRWSTVWLPGLRHVFYGRFVSGFVVA
                     AVFAASALALWVRGNVFPYWDSVEFNTPLWKWIVPGVGIVV"
     gene            2135..3439
                     /gene="asnS"
                     /locus_tag="VEC56_10080"
     CDS             2135..3439
                     /gene="asnS"
                     /locus_tag="VEC56_10080"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HXV14540.1"
                     /translation="MSKLPIVYVKDLKAHAGQAVELRGWVYNTRSGGKIRFLILRDGT
                     GYLQCVASAADLSPEDFETLGALSQESSVIVTGTPREDKRAPGGVELTLKSVTEVQHA
                     EGFPITPKEHSSSFLLDHRHLWLRSRKQHVVLLVRHHTIAAIRDYLNSHGFINLDTPI
                     FTPNACEGTSTLFETDYFGDKAYLSQSGQLYNEATAAAFGKVYCFGPTFRAEKSKTRR
                     HLTEFWMVEPEVAFMELPELMELEEDFFCYIIARVLETCGTEIREILERDTSKLEAVK
                     KPFPRIHYKEAAKFLLEKGVPFEVGGDFGGTDETVLTESQTSPIFVHHFPTQIKAFYM
                     EPDPKEPEYCLSVDMLAPEGYGEVIGGGQRTANLELLERRIKEHNLPRAAYEWYLDLR
                     KYGTFPHSGFGLGVERTVSYLCGLSHLREAIPFPRMLNRLSP"
     gene            3462..3974
                     /locus_tag="VEC56_10085"
     CDS             3462..3974
                     /locus_tag="VEC56_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXV14541.1"
                     /translation="MKHLIVSLSILGVAVFGCAKKIDVEAERAALRQADADWNAAVGA
                     KSTDDFMKFMEPEATIMPPNEAAVSGTAAIQGWVAEMMAIPGFGFSWQATSVEVAASG
                     DLGCTVGTYDAQIPLPDGSSVADNGKYVTMWRKQVDGSWKVYVDIFNSDLPMPTSPEA
                     PDDATGAAGQ"
     gene            4050..4547
                     /locus_tag="VEC56_10090"
     CDS             4050..4547
                     /locus_tag="VEC56_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXV14542.1"
                     /translation="MLLPLLVLALSGCAQKQIDLEAERAALMDADKAWAATLAEKSVD
                     GFTSYFTADAIVMPPHLPILVGIDAVRAWATESMALPGFSVTWTVTSADVAASGDMGY
                     TLGTFVFQATMPDGSTITDPGKYTTIWKKQADGSWKVAVDTFNSDSSFMPSAPAAGDT
                     TGVGQ"
     gene            4623..5081
                     /gene="merB"
                     /locus_tag="VEC56_10095"
     CDS             4623..5081
                     /gene="merB"
                     /locus_tag="VEC56_10095"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HXV14543.1"
                     /translation="MSTLLTPVKLAIYDTFARTTKAPTVFDIAKQLDAPVNQVQAAFD
                     ELHKQRLLVPEPGDKTRIRMAPPFSGIETSFRVIVGTRGEEKTYFAPCAWDSLGIPAA
                     LHKDAVVHAGDGHTGEAMTLVVRDGAPVPTPCVIHIAVPAAHWWDDIIYT"
BASE COUNT          933 a         1622 c         1678 g          860 t
ORIGIN      
        1 accacgatgc ccacgcccgg cacgatccac ttccacaagg gtgtgttgaa ctcgaccgaa
       61 tcccagtacg ggaacacgtt gccgcgtacc cacagcgcga gcgcgctggc cgcgaacacc
      121 gccgccacca cgaagccgga cacgaaccgg ccgtagaaca cgtgccgcag tccgggcagc
      181 cacaccgtcg accagcggat cgacttgcgc cggcgcacga tcaccgactg gcggcgctgg
      241 cgcaggaggg cctccagcac gcggtccgac gagacgcccg cgaccgcttc gccgcacgcg
      301 tggcagatga cgctgccgag ctcgtcgttg cagcagccct cgcacgtcag gtcgccgcag
      361 ttggcgcact ggaacgcgag cttgcggctt ttgagcgttt tgctcacgac cacggccagg
      421 atcatggcga ccaacatgat gccggcgctc accgtcgtcg ggtggccggt cacggtctcc
      481 aggagatggg tcacgagcgc ggggttgtgg tacttgccct ccaccgccgc gatcttccag
      541 atctgcgcgc tcgagtaggt cttcgggtac acggccatgc gcgcccgcgc gggctcggcg
      601 atggcgtcgc gcgcgtgttc gagccccagc ttcgacgcgc gcgagagcgc ttgcgacgat
      661 tcgcccatga gcagcgtctt gatgtacgcc tgcgcgaggt tgtactggct caccgcgtcg
      721 gtcgagtcga cttcttcggc tttgcgataa ccttcgagcg ccttgttgaa ctggccgttc
      781 tggtagtaga cgttgcccag gttcacatac gcgatggcgg agttcttgtt gatcgagatc
      841 gcgcgcagga agttcgccgc cgcggtctcg gagtcgccgt cgcgcatggc cagcatgccc
      901 agagcggtat agcgatcgtc gtcgatccgg gggtcgctca tgggggccgc ggagatggca
      961 cgcgcgagga cgtcgtcggc gggcgattcg ttggcgcgcg cgatcagcga ggtgagcgag
     1021 ccgggatccg ccacgatgga gaagcggtcg atcaggggcg aaagaccggt gaacgccgcg
     1081 aacagacccg ccatcaccat cgagagcacg cgctcgcgct tgtgcatgaa cggccaggtg
     1141 agtacgagaa tgagcgccgc cgccgaggtg tagcccggga gcagcgcgaa cggtgcgagc
     1201 agcaggaggg ccgccgccac gcggccgccg gtggcattga acttggtgcg cgccgactcc
     1261 gcgatgcggt gcgcgatgaa cgggaagtag cgaaccgcga gcgcgatcca cacgatcgcg
     1321 gacgcgagca agagcaccag cgtggccccg accaccgcgt tgagcgcgaa cgcgcgctgg
     1381 ccgtcgaagg tcttgagctg caccatgacg ccttcgacca tgtaatagac cgcatcgggg
     1441 ctgaagcgcg cggcgtgcag gcgcgcgagt gtgaagtacg cgtcgctgta tcgcggggcc
     1501 agcgagagcg cggtacgcaa acgcttctcg gcgccttcct tgtcgccttg cgcgatggcc
     1561 gccagcgcct ggcggtactc cacttgggcg gcgaccggca tcgtcaccgc gccgcgacga
     1621 agctgcgcct cttcgtaggc caccaagtga cgggcaaaat ctgccggggg ggccacgacc
     1681 ggggacgctt ccgcgaccgc cgcggtggct tgcgtatcga ccggcgccat tgtgatcggg
     1741 acgacgggtt gcgcggcgac cgtgtcgcga ggggtcggcg ccggagccgc ggccgaatcc
     1801 gcccgttgcg acccgtcggc gaagaaggcg tccaagtcgg aggggttgaa ggtggtatcc
     1861 gccggggcct cttcggcgtg cgcaaagcac gcggacgcga ccagaatcgc gacccagatg
     1921 cagaatgcaa ccacgtcccg acggcgcagg cccatcggcc cgcccgcact acctagccgg
     1981 aggtgtgtat tcatcttcgt tttcgacccg atcccggtac cggcaccgtg ccttcgcggt
     2041 gctctaagtt cttgggttcc aaggcaatcg gcaagaagcg tgccgaggtt cgggcgtctg
     2101 gcgggcccgc aacgggcgtg ttaaactgcc gcccatgtcc aaactaccca tcgtctacgt
     2161 caaggacttg aaagcccacg ccggccaggc cgtcgagctg cgcggctggg tgtacaacac
     2221 gcgttccggc gggaagattc gctttctcat tctccgcgac ggaaccggct atctccagtg
     2281 cgtcgccagt gcagccgatc tttcgcccga ggatttcgaa accctgggcg cgttgtcgca
     2341 ggaatccagc gtgattgtca ccggcacacc gcgcgaagac aagcgcgcgc ccggcggcgt
     2401 cgagctgaca ctcaagtccg tcaccgaagt gcagcacgcc gagggtttcc cgatcacgcc
     2461 caaggagcac tcgtcgtcgt ttctattgga tcaccggcat ctgtggctac gaagccggaa
     2521 gcagcacgtc gtgctgctcg tgcggcatca caccatcgcg gccattcgcg attacttgaa
     2581 ctcccacgga ttcatcaacc tcgacacgcc catttttact cccaatgcgt gcgaagggac
     2641 gagcaccctc ttcgagaccg actatttcgg cgacaaggcg tacctttcgc agagcgggca
     2701 gctgtacaac gaagccaccg cggccgcgtt cgggaaggtg tattgcttcg ggcccacgtt
     2761 tcgcgcggag aagtccaaga cgcggcgcca cctcacggaa ttctggatgg tggagcccga
     2821 ggtcgcgttc atggagctgc cggagctgat ggagctcgag gaggacttct tctgctacat
     2881 catcgcgcgc gtgctggaga cgtgtggcac ggagatccgc gaaatcctcg aaagggacac
     2941 atcgaagctc gaagcggtga agaagccctt cccgcgcatc cattataagg aggcggccaa
     3001 gttcctcctc gagaaggggg tgcccttcga agtgggcggg gacttcggcg ggaccgacga
     3061 aaccgtgctg acggaatcac agacgagccc gatcttcgtg catcattttc cgacccagat
     3121 caaggccttc tatatggagc ccgaccccaa ggaacccgag tactgcctct cggtcgacat
     3181 gctggcgccc gagggctacg gcgaagtgat cggcggcgga cagcgcaccg ccaatctcga
     3241 gttgctggag cggagaatca aggagcacaa tctcccgcgc gccgcctacg agtggtacct
     3301 cgatctgcgc aagtacggga ccttccccca ctccggcttc ggactgggcg tcgaacgaac
     3361 cgtgtcgtac ctgtgcggtc tctcgcacct gcgcgaagcg attcctttcc cgaggatgtt
     3421 gaatcgattg agtccatagg ccccggattg tggagggttt tatgaagcac cttatcgtgt
     3481 cgctatcaat attgggtgtc gccgtgttcg ggtgtgcgaa gaagatcgac gtcgaggcgg
     3541 agcgagccgc gcttcgacag gccgatgcgg actggaatgc ggcggtgggg gcgaagagca
     3601 cggacgattt catgaagttc atggagcccg aggcgacgat catgcccccg aacgaagcag
     3661 ccgtctcggg aacggcagcc atccaaggct gggtggcgga gatgatggcg attcccggct
     3721 tcggtttcag ttggcaagca acgagcgtcg aagtggcagc gtcgggagac ctgggatgca
     3781 ccgtgggaac gtatgacgcg cagatcccgt tgcccgacgg ttcgtcggtg gccgacaacg
     3841 gcaagtacgt gacgatgtgg aggaagcagg tcgatggctc atggaaggtc tacgtcgaca
     3901 tcttcaactc ggatctcccg atgccgacgt cgccggaggc tcccgatgat gcgacgggag
     3961 cggcggggca gtagccggtt gccgatcgaa ttcgtgcgcc cgcacggacg gttctaaaga
     4021 ggaggattgc catgcgaacg cgcgttctta tgttgttgcc tcttttggtg ctcgcgttgt
     4081 ccggctgtgc gcagaaacag atcgacctcg aagccgagcg cgcggcactc atggatgccg
     4141 acaaggcgtg ggcggccacg ctggccgaga agagcgtcga tggtttcacc agctatttca
     4201 ccgcggatgc cattgtcatg ccgccgcacc tccccattct cgtgggcatc gatgccgtgc
     4261 gcgcgtgggc gaccgagagc atggcactac ccggtttctc ggtaacgtgg accgtgactt
     4321 cggccgacgt cgccgcgtcg ggcgacatgg gctacaccct gggcacgttc gtcttccagg
     4381 ccacgatgcc cgacggctcc accatcaccg accccggcaa gtacacgacc atttggaaga
     4441 agcaggccga cggttcgtgg aaggtcgcgg tcgacacgtt caactcggac agctcgttca
     4501 tgccgagcgc tccggcggcc ggggatacga cgggagtcgg gcagtagctt catcccataa
     4561 ccgcctgcgc gagctatcgg agagcgacca tccgggagca cggacgtcgc cggaggtgtc
     4621 gcgtgtcgac gttactcacc cctgtcaaac tcgccatcta cgacaccttc gccaggacga
     4681 cgaaggcgcc caccgtcttc gacatcgcga aacaactcga cgcgcccgtc aaccaagtcc
     4741 aagccgcctt cgacgagctc cacaaacagc gcctcctcgt tcccgagcct ggtgacaaga
     4801 cgcgcattcg catggccccg ccgttctcgg ggatcgagac gtcgtttcgc gtgatcgtcg
     4861 ggacgcgggg agaagagaag acctacttcg ccccgtgtgc gtgggactcg ctcggcatcc
     4921 cggcggcact ccacaaagac gcggtcgtcc acgccggcga cggacacacg ggcgaggcga
     4981 tgacgctggt cgtgcgcgac ggggcgccgg tcccgacacc gtgcgtgatc cacattgccg
     5041 ttcccgccgc gcattggtgg gacgacatca tctacacctg aagcacgatg ctc
//
LOCUS       DASPGK010000363         8782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1493369, whole genome shotgun sequence.
ACCESSION   DASPGK010000363 DASPGK010000000
VERSION     DASPGK010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493369"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VEC56_10100"
     CDS             <1..1380
                     /locus_tag="VEC56_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14544.1"
                     /translation="VVGASLCSDEARALPQYAARAGRSCDNCHILPDWDTPKFSERKR
                     NLSCQTCHVDPAGGGMRTTAGRYYGRATLAMIATSPRPMDDWDRNFPGLGRRDKATPY
                     DDYMPVGPANFEHAAAYRDSLHDKYAWGTPKGDTRYGPFAGRFKELNPDPVLRIGADL
                     RFATLVSQSTLMFPMQIDVGTALHPVHHLTALMNIGARGQSSGYSDTFDDSHSPYFRE
                     LFAMTNEWPYQAYAKAGRFVPSYGLRLDDHTNRIRREFELDGSLPDSRVLGVEVGAVA
                     AYPFLQASYFKLKSKFEEPDPWDIFDVDDGWGAALNLGWRHFGWSAGASAMMRRRPLD
                     EGGDTDTFGLYGSFNPWYFSSRIPLTLQGEVDIGTVQRASGREADKLVWYGELGWLVW
                     NGVNLIAAYDWTDPDRDVIDDHSGRVQFGGQLTPIPGVTFDARVRYLDVATESGNGTD
                     LFLQMHIWF"
     gene            1483..2157
                     /locus_tag="VEC56_10105"
     CDS             1483..2157
                     /locus_tag="VEC56_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV14545.1"
                     /translation="MKKLASCLLVVAIAASQLSCSSMSNTQKGAIGGAAAGGAIGGII
                     GHQSGNTAVGAIIGAAVGGAAGAWIGSYMDKQAAEIQRDLEGAKVERIGEGIKITFDS
                     GLLFDVNRAVLKPASQQNLTELAVILNKYPDTNILLEGHTDSDGSEEHNLNLSELRSK
                     SVANFLATQQVMETRFTTMGYGESQPIADNSTASGKAKNRRVEVAIYANDKLKKQAEK
                     KVAEGG"
     gene            2162..2821
                     /locus_tag="VEC56_10110"
     CDS             2162..2821
                     /locus_tag="VEC56_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXV14546.1"
                     /translation="MLQTGTANEKIERAVRDVLADGVFLCVRLGAGAPLVDACRAAVR
                     GGLTLLEITLTTPGALDAIAALSRDHGVVAGGGTVLTRDDARAVADAGGRFALSPAFD
                     PEVVEEAHRLGLLAVPGAATATEILAAHRSGARLVKVFPAAALGGPAYLRAIRGPLPN
                     IPLVPTNGITAATIREYIDAGAAAVGVGGEVFPEGFTLQHVESAARRIRQAMDAARSA
                     R"
     gene            complement(2818..3528)
                     /locus_tag="VEC56_10115"
     CDS             complement(2818..3528)
                     /locus_tag="VEC56_10115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14547.1"
                     /translation="MSSPARNPVDHSLTYRGGLRNLPHRRRLRAIVAALTRGGAPAIT
                     SFADVGCSNGYVTAILQARLGPARTVGFDHVESHLERGRREHPQIEFRSIDLNRPLPA
                     GHETFDLVTCFETLEHVGRLEPAIANLLAMTRAGGTLFITIPIESGPRGLLKFLIKVG
                     VFRYSLDELPARPRRFWSYLGALAGNRTIASFRDQRDGWGTHFGFDWREVERILRANG
                     AAFESRSDFTTRFVVVRR"
     gene            complement(3539..3730)
                     /locus_tag="VEC56_10120"
     CDS             complement(3539..3730)
                     /locus_tag="VEC56_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HXV14548.1"
                     /translation="MSGRKSFLVRLPEELLDELNRWARDDLRSLNGQIEYLLREAVRK
                     RRGVSLPDSAPPEGPVKPA"
     gene            complement(3745..4617)
                     /locus_tag="VEC56_10125"
     CDS             complement(3745..4617)
                     /locus_tag="VEC56_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXV14549.1"
                     /translation="MKTRESEIKSMNGWAMLVLLLGAGVALTVWFIEIVKTGPTATFI
                     VVWSIAMIVWAALLGGFFTLQPNMAAVLVLFGRYSGTVKTSGFHWANPFFSKTRVSLR
                     ARNLNAERIKVNDSRGNPIEIGAVIVWSVANTAQAIFDVDEFVHYVTIQSESALRHLA
                     SAYPYDTTEEGQLSLRGSMDEVSETLTKELQERVHLAGVKIDEARLSHLAYAPEIAHA
                     MLQRQQAEAVVAARTRIVDGAVGMVEMALERLDKHSTVKLDEERRAAMVSNLLVVLCG
                     DKAAQPVVNTGTLY"
     gene            complement(4726..5562)
                     /locus_tag="VEC56_10130"
     CDS             complement(4726..5562)
                     /locus_tag="VEC56_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HXV14550.1"
                     /translation="MKTLSIGHSPDADDAFMFYGLASGRVAVPGYRVDHVLDDIETLN
                     RRAHAAELDVTAISAAAYPQVQHHYRIMSCGASVGRRYGPIVLARDAMTIADLAGKRI
                     AVPGPHTTAYLLFRLYVEAEIVPVMMDFDRIMDAVSSGDVDAGIVIHEGQLTWPSSGL
                     RLVVDLGAAWTEETGLPIPLGLDVVRRGLGGATMDAVARALKESILLAHGDEEAAVDY
                     AMRFGRGVDRETCRKFVRMYVNDDTVDMGDDGRRALETLYGRAVHRGLLATPPNLDIL
                     WV"
     gene            complement(5573..6136)
                     /locus_tag="VEC56_10135"
     CDS             complement(5573..6136)
                     /locus_tag="VEC56_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14551.1"
                     /translation="MTTRRLTSSMSARPTRPWMDELADRVPGGSRNVKIGLGVLAGVI
                     LALIIAQIVTSQRVAQWASIEVVDSRDPLQDLPIRLASSDGTVRPAQTYDAILDLKSI
                     DHLVVGVDLDYLPRPGTRREVVIRTGDGVEKFRDEVPGSYFTEGRFMLRLFSRQFPSG
                     DYTLEIEAPEESGETRVVAASWFQVER"
     gene            6172..6819
                     /locus_tag="VEC56_10140"
     CDS             6172..6819
                     /locus_tag="VEC56_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV14552.1"
                     /translation="MRPGERPNPAGGPVPRVVGIAGPSGSGKTTLARALAERSGGIVF
                     ALDAYYRDQRGRSHATIQVDVPDAIDIDLAVAHLRDLVAGSAVDQPVYDYATHSRTEA
                     TRTLDPAPFVLVEGLFALYWTELRALCQTRVFVALDHDECLRRRIERDVRERGRTPAD
                     VTAQYERVVRPMYEQHVAPTRSHAHLVLDGRASLVQLTESVMDALAPRSEGEAQG"
     gene            complement(6796..7023)
                     /locus_tag="VEC56_10145"
     CDS             complement(6796..7023)
                     /locus_tag="VEC56_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14553.1"
                     /translation="MMERIRSGLAQFRRDLRSPLPDHERLTRRERWRVRSRLLVGKYG
                     WKLVLGVFVYYLIRDLTLYVLLPYLALSFAF"
     gene            complement(7314..8462)
                     /locus_tag="VEC56_10150"
     CDS             complement(7314..8462)
                     /locus_tag="VEC56_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXV14554.1"
                     /translation="MRTIYLDNNATTPVAPAVFEAMRPYFTEHYGNPSSPHHMGDKPA
                     SAVRDARARVASFLGCTDAEVVFTSCGTESNNIAIRGALDSAKGHHVITTAVEHSAVM
                     NPVKRLGSTGFETTVLSVDRGGRANLDELRESLRDDTALVSVMFANNETGVIFPIDRI
                     AEIVHARGIPLHVDCVQGVGKVPIDLRTLGADLVSFSAHKFHGPKGVGALVVRKGTRW
                     APVFLGGGQERNRRPGTENVPGIVGMAAACDYAETHLAHCQTETRRLRDLMESELLER
                     VPDAFVNGVASERLPNTSNIGFHGVDGNALLVLLDEVGICVSAGSACKSGAGLPSHVL
                     AAMGLSPEEAAASLRFSLSALTTEDEIGYCIEQIPAIVDRMRRKFISA"
     gene            8544..>8782
                     /locus_tag="VEC56_10155"
     CDS             8544..>8782
                     /locus_tag="VEC56_10155"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXV14555.1"
                     /translation="MSESSTKKKNPKGRRVGLVIVHTGNGKGKTTAAIGTAFRAVGVG
                     FKVKMIQFIKGSWDYGELHSAQTLGNFEIVPMGEG"
BASE COUNT         1537 a         2871 c         2953 g         1421 t
ORIGIN      
        1 gtggtgggcg cgtccctctg cagtgacgaa gcccgtgcgc tgccgcagta cgcggcgcgc
       61 gcggggcggt cctgcgacaa ctgccacatt cttcccgatt gggacacgcc gaagttctcc
      121 gagcgcaaac ggaacctctc gtgccaaacc tgccacgtcg acccggccgg cggcggtatg
      181 cgcacgacgg cgggacgcta ctacggtcgc gccacgctcg cgatgatcgc gaccagcccg
      241 cgcccgatgg acgattggga tcgcaatttt ccggggctgg gccggcgcga caaagcgact
      301 ccctacgacg actacatgcc cgtcggtccc gccaacttcg agcacgcagc ggcgtacagg
      361 gactcgctcc acgacaagta cgcctggggg acgcccaagg gcgacacgcg ctacgggccg
      421 ttcgcgggcc gcttcaagga gctcaacccc gacccggtgc ttcgcatcgg cgcggatcta
      481 cgcttcgcga cgctggtgtc gcagtccact ctaatgtttc cgatgcaaat cgacgtgggc
      541 accgcgctcc atcccgtcca tcacctcacc gctctcatga atattggcgc gcgcggccaa
      601 tcgagcggtt attccgacac cttcgacgat tcgcactcgc cgtacttccg cgagttgttc
      661 gccatgacga acgagtggcc ctaccaggcg tacgcgaagg cggggcgctt cgtgccgtcg
      721 tacggcctgc gcctggacga ccacaccaac cgcatccgcc gcgaattcga gttggacggc
      781 tcgctgcccg attcgcgcgt gctcggggtg gaggtgggcg cggtcgcggc ctatccgttc
      841 ttgcaggcgt cgtacttcaa gttgaaatcg aaattcgaag agccggatcc gtgggacatc
      901 ttcgacgtcg acgacggctg gggcgccgcg ctcaacctgg gctggcgcca cttcggctgg
      961 tcggcgggcg cgagtgccat gatgcgacga aggcccctcg acgaaggcgg cgacacggat
     1021 accttcggcc tgtacggctc gttcaacccg tggtacttct cgtcgcgcat tccgctgacc
     1081 ctgcagggcg aagtcgatat cgggaccgtg cagcgcgcga gcggtcgcga ggccgacaag
     1141 ctggtgtggt acggcgagct gggatggctg gtctggaacg gggtcaacct gatcgcggcc
     1201 tacgactgga ccgaccccga tcgcgacgtg atcgacgacc attcgggccg cgtccaattc
     1261 ggcggccagc tgaccccgat tcccggcgtc accttcgacg cacgagtgcg ctacctcgac
     1321 gtcgccacgg agtcgggcaa cgggaccgac ctgtttctgc aaatgcacat ttggttctaa
     1381 cgacgccgct gtgatcccgt tcacacctgt gcgggcgcgc ggttgcgcgc gggcgtttgc
     1441 gatgataggc tatgggcgaa gccctaactc tctggaggaa tcatgaagaa attagcgagc
     1501 tgcctattgg ttgtcgccat cgctgcgtcc caattgagct gcagcagtat gagcaatacg
     1561 cagaagggcg ccatcggggg cgccgcggcc ggaggcgcga tcggcggcat catagggcac
     1621 cagagtggaa acaccgcggt cggtgccatc atcggcgcgg ccgtcggcgg cgcggcgggc
     1681 gcctggatcg gcagctacat ggacaagcag gccgctgaaa tccagcgcga cctcgaagga
     1741 gccaaggtcg agcgcatcgg cgagggcatc aagatcacgt tcgactccgg gctgttgttc
     1801 gacgtcaacc gggcggtgct gaagcccgcc agtcagcaga acctcaccga gctggccgtg
     1861 atcttgaaca agtaccccga caccaacatt ctcctcgagg gccacacgga ttcggacggc
     1921 tccgaggagc acaatctgaa tctctcggag ttgcgctcga agtcggtggc gaatttcctc
     1981 gccacgcaac aggtgatgga gacgcgcttc acgaccatgg gctacgggga gtcgcagccc
     2041 atcgccgaca actccaccgc ctcgggcaag gcgaagaatc gccgcgtcga agtggcgatc
     2101 tacgccaacg acaagctcaa gaagcaggcc gagaaaaagg tggccgaggg cggatagacg
     2161 cgtgctgcag acgggcaccg ccaacgagaa aatcgaacgc gccgtccgcg acgtgctcgc
     2221 ggacggcgtg tttctgtgcg tgcggttggg agccggcgcg ccactggtcg acgcgtgtcg
     2281 cgcggcggtg cgcggcgggc tgacactgct cgagatcacg ctgaccacgc cgggtgcttt
     2341 ggacgcgatc gcggcgctct cgcgcgacca cggcgtggtg gccggtggcg gcaccgtgct
     2401 cacgcgcgac gacgcgcgcg cggtcgcgga cgcggggggc cgcttcgcgc tttcgcccgc
     2461 gttcgacccc gaggtggtcg aagaggcgca ccgtctcggg ctcctcgcag tccccggggc
     2521 ggccacggcg accgagatcc tggccgctca ccgttcgggc gcacggctgg tgaaggtgtt
     2581 tcccgccgcc gcgctgggcg gcccggccta cctgcgcgcg atccgcggcc cgctgccgaa
     2641 cattcccctg gttccgacca acgggatcac cgccgcaacc atccgtgagt acatcgacgc
     2701 gggggccgca gcggtgggcg tgggtggaga ggtgttcccg gaaggattca cgctccagca
     2761 cgtggagagc gccgcgcgcc gcatccggca ggcgatggac gcggcgcgtt cggcccgtta
     2821 gcggcgcacg accacgaagc gcgtcgtgaa gtcgctgcgc gactcgaacg ccgcgccgtt
     2881 cgcccgcagg attctctcga cttcccgcca atcgaaaccg aagtgggtgc cccatccgtc
     2941 gcgctggtcg cggaacgacg cgatcgtgcg gttgccggcg agcgcaccga ggtagctcca
     3001 gaaacgacgc gggcgcgccg gcagctcgtc cagcgagtat cgaaacactc ccaccttgat
     3061 caggaacttc aagagcccgc gcggccccga ttcgatcgga atcgtgatga agagcgtccc
     3121 gccggcgcgc gtcatcgcga gcaagttggc gatggccggc tcgagtcgtc ccacgtgctc
     3181 gagtgtctcg aagcaggtaa cgaggtcgaa ggtctcgtgg cccgcgggaa gcgggcgatt
     3241 gaggtcgatg gatcgaaact cgatctgcgg atgctcgcgg cgcccgcgct cgaggtgaga
     3301 ctccacgtgg tcgaagccga ccgttcgtgc ggggccgagc ctcgcctgca agatcgcggt
     3361 gacgtagccg ttgctgcacc cgacgtcggc gaaggaagta atcgcgggcg cgccgccgcg
     3421 cgtgagcgcg gccacgatgg cccgtaatcg gcgccggtgc ggcagattcc gcaatccgcc
     3481 ccggtaggtc agcgaatgat cgacaggatt gcgcgccggg ctcgacatcg gtatttcctc
     3541 acgccggctt gaccggcccc tcgggcggtg cggaatcggg cagcgacaca ccgcgccgct
     3601 tccgcaccgc ttcgcgcaag agatactcga tctgaccgtt caggctgcgc aagtcgtcgc
     3661 gcgcccatcg attgagctca tcgagcagct cctccggaag tctgacgaga aatgattttc
     3721 ttccggacat gatcgctcac gatcctagta tagcgtgccc gtgttgacga ccggctgcgc
     3781 cgctttgtcg ccgcacagca cgacgagcaa gttgctgacc atcgccgcgc gacgctcttc
     3841 gtcgagcttg acggtcgaat gcttgtcgag acgttcgagc gccatctcga ccatgccgac
     3901 ggcaccgtcg acgatgcgcg tgcgagccgc gacgaccgct tcggcctgct ggcgctgcag
     3961 catggcgtgc gcgatctcgg gggcgtacgc gaggtggctc aagcgggcct cgtcgatttt
     4021 gacgcccgcc aggtggacgc gctcctgcag ctccttggtg agcgtctcgg agacttcgtc
     4081 catgctgccg cgcagcgaca gctggccttc ctccgtggtg tcgtaggggt acgcgctggc
     4141 caggtgccgc agcgccgact cgctctggat ggtgacgtag tgcacgaact cgtcgacgtc
     4201 gaagatggcc tgcgcggtgt tggcgacact ccacacgatg accgcgccga tctcgatcgg
     4261 gttaccgcgc gagtcgttga ccttgatgcg ctcggcgttc aagttgcgcg cgcgcagcga
     4321 aacccgcgtc ttcgagaaga agggattggc ccagtggaag ccgctggtct tgacggtgcc
     4381 ggagtagcgt ccgaagagga cgagcacggc cgccatgttg ggctgcaatg taaagaagcc
     4441 gccgagcagc gccgcccaca cgatcatcgc gatggaccag acgacgatga acgtcgccgt
     4501 gggacccgtc ttgacgattt cgatgaacca aacggtcagc gcgacgcccg cgcccaatag
     4561 caggacgagc atcgcccatc cgttcatgct cttgatttcg ctctcccgcg ttttcatacg
     4621 ggcaacctcc ttgagtaagc tagcgataca atatcagaat gatatcatgt ggtcaagagg
     4681 gatttttagg ctgcctaaca gcctgcgcga gaacgggtta ggtgattaga cccagagaat
     4741 gtcgaggttg ggaggggtgg ccagcagccc gcggtggacg gcccggccgt acagggtctc
     4801 gagcgcgcgt cgaccgtcgt cgcccatgtc gacggtgtcg tcgttgacgt acatgcggac
     4861 gaatttgcgg cacgtctcgc gatcgacgcc ccgcccgaaa cgcatcgcat agtcgacggc
     4921 cgcctcttcg tcgccgtgcg ccaacaggat gctctccttg agcgcgcgcg ccaccgcgtc
     4981 catggtggcg ccgcccaagc cccgccttac aacatccagc cccagcggga tcgggagccc
     5041 cgtctcttcc gtccacgccg cacccagatc gacgaccagg cgcaggccgc tcgaaggcca
     5101 cgtgagctgg ccctcgtgga tcacgattcc cgcatcgaca tcgccgctcg agaccgcgtc
     5161 catgatgcgg tcgaagtcca tcatgacggg cacgatttcg gcttcgacgt acagacgaaa
     5221 caggagatag gccgtggtgt gcgggccggg gacggcgatg cgcttgcccg ccaggtcggc
     5281 gatcgtcatc gcgtcgcgcg caaggacgat gggaccgtac cggcggccga ccgacgcccc
     5341 gcacgacatg atgcgatagt ggtgctggac ctgggggtag gccgccgccg agatcgcggt
     5401 cacgtccaac tcggcggcgt gggcgcgccg attcaacgtt tcgatgtcgt ccagcacgtg
     5461 atcgacgcgg tagccgggaa cggccacgcg cccggacgca agcccgtaga acatgaaggc
     5521 gtcgtccgcg tcggggctat ggccgatgga gagggtcttc atcgaaggtc gtctagcgct
     5581 cgacctggaa ccaggaggcc gccaccacgc gcgtctcgcc ggattcctcg ggggcttcga
     5641 tctcgagcgt gtagtcgccc gaagggaatt ggcgcgagaa cagcctcagc atgaagcggc
     5701 cctcggtgaa ataggagccg gggacttcgt cgcgaaattt ttcgaccccg tcaccggttc
     5761 gaatgacgac ctcgcgcctc gtgccgggcc gcggcagata gtccagatcg acaccgacga
     5821 ccaagtggtc gatcgatttg agatcgagga tggcgtcgta ggtttgtgcc ggtcgcacgg
     5881 tgccatccga cgaagccagt cgaatgggaa ggtcctggag cgggtcgcgc gaatcgacga
     5941 cctcgatcga cgcccactgg gccacgcgct gcgacgtcac gatctgggcg atgatgagcg
     6001 cgaggatcac gcctgccagc acgcccaggc cgatcttgac gttgcggctc ccgcccggca
     6061 cccggtcggc cagctcgtcc atccacggtc gggtgggtcg ggccgacatg ctggaggtga
     6121 ggcggcgggt ggtcatgccc ccatgctagc acaaagtggt acgatcgcgt catgcgaccc
     6181 ggagaacgac cgaacccggc cggcggaccc gtccctcgtg tggtcgggat cgccggtccc
     6241 tcggggtccg gcaagaccac gctcgcccgg gcactcgcgg agcggtcggg ggggatcgtg
     6301 ttcgcgctgg acgcctacta ccgcgaccag cgaggtcgct cccacgcgac gatccaggtc
     6361 gacgtccccg acgcgatcga catcgacctg gcggtcgcac acctgcgcga tttggtcgcc
     6421 gggtcggccg tcgaccagcc ggtctacgac tacgccaccc actcccgcac cgaagcgacg
     6481 cgcaccctcg atcccgcgcc gttcgtcctc gtcgaggggc tcttcgcgct ctactggacc
     6541 gagctccgcg cgctttgtca gacccgcgtg ttcgtcgccc tcgaccatga cgaatgcctt
     6601 cgccggcgca tcgaacgcga cgtgcgcgaa cgcggacgca cgcccgccga cgtcaccgcg
     6661 cagtacgagc gcgtcgtgcg cccgatgtac gagcagcacg tggcacccac acggagccac
     6721 gcgcacctcg ttctcgacgg gcgcgcgagc ctcgtccagt tgaccgagag tgtcatggat
     6781 gcactcgcgc cccgctcaga aggcgaagct caaggctagg taggggagca ggacgtagag
     6841 cgtcaggtcg cggatcaggt agtacacgaa gaccccgagc acgagcttcc agccgtactt
     6901 tccgaccaga aggcgtgaac gaacccgcca gcgctcacgc cgggtgagcc gctcgtggtc
     6961 gggaagcggc gaccggaggt cgcgacggaa ctgtgccaga ccgctgcgta ttcgttccat
     7021 catggccgtc acaccaaccc aagggtacca aagccggaat ggaaaacaaa aggcccccgg
     7081 tggaggaccc ttcgttggtg cttcggaagg aaggcgaggc tcgggcggac gggcgcggga
     7141 ccggggagta ggccccgcgg tggggcgatc gcttaatgga tccggacgcg aggggaggga
     7201 ttgacggttc ggtcactctg ggctccctcg cagtccggat cgcaaatccg tgacgaaccc
     7261 gcattccgga ttcgatacgc aagggagtct agtcgggccg tgtgaatcga gaattaagcg
     7321 gaaatgaatt tgcggcgcat gcgatcgacg atggcgggga tctgctcgat acagtagccg
     7381 atctcgtcct cggtcgtaag agcggagagc gaaaagcgca gggaggcggc ggcttcctcg
     7441 ggcgagagcc ccatcgcggc cagcacgtgc gagggaagcc ccgcgcccga cttgcacgcc
     7501 gagcccgcgg aaacgcagat gccgacttcg tccaggagga ccaagagcgc gtttccgtcg
     7561 acgccgtgga agccgatgtt ggacgtgttc gggagccgtt cggacgccac tccgttgacg
     7621 aaggcgtccg ggacgcgctc gagcagctcc gattccatga ggtcgcgcaa gcgtcgcgtc
     7681 tcggtctggc agtgcgcgag gtgcgtctcc gcgtaatcgc acgcggccgc cattccgacg
     7741 atgccgggca cgttctcggt accggggcgc cggttgcgct cctgtccgcc acccaggaac
     7801 acgggcgccc aacgcgtccc cttgcgcacc accagggctc ccacgccctt cggtccgtgg
     7861 aacttgtgcg cggagaacga caccaaatcg gcgcccaggg ttcgcagatc gatggggacc
     7921 ttgcccacgc cctgcacgca gtcgacgtgg agcgggattc cgcgcgcgtg cacgatctcg
     7981 gcgatgcgat cgatcgggaa gatgacgccg gtctcgttgt tggcgaacat caccgacacc
     8041 agggcggtgt cgtcacgcaa cgattcgcgc aactcgtcga ggttcgctcg gccgccgcga
     8101 tcgaccgaaa gcaccgtcgt ttcgaagccg gtcgagccca ggcgcttgac cggattcatc
     8161 acggccgagt gctcgaccgc ggtcgtaatg acgtggtgcc ccttggcgga atcgagcgcg
     8221 ccgcgaatcg cgatgttgtt cgactcggtc ccgcagctcg taaagactac ttcggcgtcc
     8281 gtgcagccca aaaacgacgc cacacgcgcg cgcgcgtccc gcaccgcgga cgcgggcttg
     8341 tcgcccatgt ggtgcgggct cgacgggttg ccgtagtgct ccgtgaagta cgggcgcatc
     8401 gcctcgaaga cggcgggcgc gacgggtgtg gtggcgttgt tgtcgaggta gatcgtccgc
     8461 atggtcaggc gaccccgccc tgtacgcggg ggccgcaagg tgctatttta ggccgcgcac
     8521 ggcctcggca caagcccaca atcatgagcg aatcgtcgac caagaagaag aatcccaagg
     8581 gacgccgcgt cggcctcgtc atcgtccaca ccggcaacgg caagggcaag acgacggctg
     8641 ccatcggtac cgcgtttcgc gccgtgggcg tgggcttcaa ggtcaagatg atccagttca
     8701 tcaagggctc gtgggactac ggcgagctgc attcggcgca gacgctgggg aacttcgaga
     8761 tcgtccccat gggcgagggc tt
//
LOCUS       DASPGK010000364         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1493447, whole genome shotgun sequence.
ACCESSION   DASPGK010000364 DASPGK010000000
VERSION     DASPGK010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493447"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VEC56_10160"
     CDS             complement(<1..573)
                     /locus_tag="VEC56_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14556.1"
                     /translation="MRFTSDLHSKHGLLHIDRATGRPEYAFIHGNWTLDNSDPDGMYC
                     GVNGELRVLAQTGCYADFTYPSAPHPTQPPIMNRIYYATDDPTRRRSHWKGIDAHYGR
                     AGSGTLLLVTGPLCVNWRKRRRFLPAIENGDITDLNPAVPARVDAWVRTGVHVRGFPR
                     WVFVKAYTHGGQEPNAEHLLSDRAGGLSAMY"
     gene            complement(1024..2418)
                     /locus_tag="VEC56_10165"
     CDS             complement(1024..2418)
                     /locus_tag="VEC56_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXV14557.1"
                     /translation="MSNQRITVATTLAAVGLAAFGVGYLLLQSTSMGAAAAVALIGMT
                     VIFVDPFVGLVTYLLFLYTRPQDYVLALQNMPIMVALSVATFGLMVLHMAVRHRMIVL
                     AKVPQNLLMVWFYAAIIMSHVVWLNPATTIEATMEFLPTLVMYFLVSTLVSTHGKLKF
                     TVNLIVIGTLFLAITGFIQYFTGTGLGGKETYEGRIQAVGIFSDPNDLGLALVVVLPF
                     FYLKLTEDSKLWQKPLALIGLGIILYALFLTQSRGGILALGVILVLLLTRRLGRTFGM
                     VTGAVVFLAIFALAPRMGSISTEEASAHGRVEAWSVGLDLFETYPIFGVGMNNFTQYH
                     FRTAHNSFVLCATELGMFGLYPWIMLIYLSIKNNEFVASAVRGMRMRDVAVYVETIRY
                     GLIAFSVGAYFLSRTYNEVLFLFVGLSAAVTNIFVKESSERYLLIEKRDWINGLLWTA
                     GAWVITKMFLIATW"
     gene            complement(2411..>3192)
                     /locus_tag="VEC56_10170"
     CDS             complement(2411..>3192)
                     /locus_tag="VEC56_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14558.1"
                     /translation="LRQPKGRVNLAAQRALERWVLFGCDRVVANTIGNRDALLSAFPR
                     LDPARVRVVPNAFDTETMPPAADAGDPALACDIAYFGEIYPGMLDAYFEALRVLVARE
                     AASAPRLHVFGRVDAADVHRVAQSGLGSHIVFKGVVSYARSLALMRSARSLLLLLPDG
                     ESMATCVPSKLYPYLFTGRPVLAFVPPGDAARVVEETDSGEVVTPGEPAATAERLRAF
                     VERVRRGESGGAGRGNARDRYAMDRVAGDIHAILEEVAARV"
BASE COUNT          579 a         1045 c         1076 g          492 t
ORIGIN      
        1 atacatggcc gacaggccgc ccgcgcggtc cgacagcagg tgttcggcgt tcggctcctg
       61 gcccccgtgg gtgtaggcct ttacgaagac ccagcgcgga aagccgcgca cgtgcacgcc
      121 cgttcgaacc cacgcgtcta cgcgcgccgg cacggcggga ttcaagtcgg taatgtcgcc
      181 gttctcgatc gccggcagga aacgccggcg cttcctccaa ttcacgcaca gcggtccggt
      241 caccaagagc agcgtccccg agcccgcgcg cccgtagtgg gcgtcgatcc ccttccagtg
      301 cgagcgccgc cgggtcgggt cgtcggtggc gtagtagatc ctgttcatga tgggcggctg
      361 cgtcgggtgg ggcgcggacg gataggtgaa gtcggcgtag cacccggtct gcgcgagcac
      421 gcgcaactcg ccgttgacgc cgcagtacat cccgtccggg tcggagttgt cgagcgtcca
      481 gttgccgtga atgaacgcgt actcggggcg gccggtcgcg cggtcgatgt gcaggagccc
      541 gtgcttcgaa tgcaggtcgc tcgtgaagcg caccagcttc tcgcgcaggc cctcggcggt
      601 gtcccggtcg tggtgcaggt ggacctctac ctcgcccagg cgcgcgcgcc agcgtcgcga
      661 gcatgtcgag acaatgcggg tcgtactctt cctgcgggta gaagaaggta tgtcgcggcg
      721 cgttgccgcc gttgtcgtgg atcccttcca ccgcgcgcgg gtagccgtcg atccaccggc
      781 gcacgcgttc gtgggccacg gcacggccgg gattgagcca atagggctcg aagtggtccg
      841 ccacgcacac gaagatgtgc gtggtgccct cggcggccat cctggggcgc gcgaacgcgg
      901 cccgcgcgat gcggtcgacg ttcttgcgcg atagcacgcg gtacgcccgc agtccgccga
      961 cggcgaccgc cgccgatagg aggatgcgaa ccaccgtggc cgcgtgtata tggatgctgg
     1021 ctgctaccac gttgcaatga gaaacatctt ggttataacc cacgcgcccg cggtccacaa
     1081 cagtccgttg atccagtccc gcttttcgat caacaggtaa cgctcgctgc tctccttgac
     1141 gaagatgttg gtcacggccg cggagagacc cacgaacagg aacaggacct cgttatacgt
     1201 gcgtgatagg aaatacgcgc ccaccgagaa cgcgatcagc ccgtatcgaa tcgtctcgac
     1261 gtagaccgcc acgtcgcgca tccgcattcc gcgcaccgcg ctcgccacga actcgttgtt
     1321 cttgatcgac aggtagatga gcatgatcca ggggtagagc ccgaacatcc ccagctcggt
     1381 ggcgcacagc acgaaggaat tgtgcgcggt tcgaaaatga tattgcgtga aattgttcat
     1441 acccacccca aatatggggt aggtctcgaa caggtcgagc cccacgctcc acgcctcgac
     1501 ccggccgtgc gcggacgctt cctcggtcga gatgctgccc atgcgcgggg ccagcgcgaa
     1561 gatggcgagg aataccaccg cgccggtgac catgccgaac gtcctcccca gccgccgtgt
     1621 caacagcagg accaagatca cgcccagcgc gaggatgccg ccgcgcgact gggtcaggaa
     1681 gagcgcgtac agaatgatgc ccaggccgat cagcgcgagc ggcttctgcc acagcttcga
     1741 gtcctcggtg agcttcaaat agaagaacgg gagcacgacc accagcgcga gccccaggtc
     1801 gttgggatcg gagaagatgc cgacggcctg aatgcgtccc tcgtaggttt ccttgcctcc
     1861 cagcccggtt ccggtgaagt actggatgaa gcccgtgatg gcgaggaaca acgtcccgat
     1921 gacgatcaga ttgaccgtga acttgagctt gccgtgggtc gacaccaacg tggagacgag
     1981 gaagtacatc accaaggtcg ggagaaactc catggtcgct tcgatggtcg tggcgggatt
     2041 gagccacacc acgtgcgaca tgatgatggc ggcgtagaac cagaccatga gcaggttctg
     2101 gggcactttc gccagcacga tcatgcgatg gcgcaccgcc atgtgcagca ccatgagccc
     2161 gaaggtggcc acactcagcg cgaccatgat gggcatgttc tggagcgcga gcacgtagtc
     2221 ctgtgggcgc gtgtagagga acaagagata cgtgaccagc ccgacgaagg ggtcgacgaa
     2281 gatcacggtc atgccgatca acgccacggc ggcggccgca cccatggacg tcgattgcag
     2341 gagcaggtag cccacgccga aggcggccag ccccaccgcc gccagcgtcg ttgcgaccgt
     2401 gatgcgctgg ttagacacgg gccgcgacct cctcgaggat cgcgtggatg tcgcccgcca
     2461 cgcgatccat cgcatagcgg tcgcgcgcat tcccgcgccc ggccccgccc gattctccgc
     2521 ggcgcacgcg ctcgacgaag gcgcgcaggc gctcggccgt ggccgccggc tcacccgggg
     2581 tcacgacttc acccgagtcg gtttcctcta ccacgcgggc cgcgtcgccg ggcgggacga
     2641 atgccagcac cgggcgtccc gtgaacagat acgggtagag cttgctcggc acgcaggtgg
     2701 ccatcgattc cccgtcgggc aagagcagga gcagcgagcg cgcggagcgc atcagtgcga
     2761 ggctgcgcgc gtacgacacc acgcccttga agacgatgtg ggatcccagg ccggattgcg
     2821 cgacgcggtg cacgtccgcc gcgtcgacgc gcccgaagac gtggaggcgg ggcgcgctcg
     2881 cggcctcgcg cgcgaccagc acgcgcagcg cctcgaagta cgcgtcgagc attccggggt
     2941 agatctcgcc gaaataggcg atgtcgcagg cgagcgcggg atcgcccgcg tcggcggcag
     3001 gcggcatggt ctcggtgtcg aaggcgttgg gcacgacgcg cacccgcgcc ggatccaggc
     3061 gcgggaaggc cgagaggagc gcgtcgcgat tcccgatcgt gttggcgacc acgcggtcgc
     3121 agccgaacaa gacccaccgc tcgagcgcgc gctgcgccgc gagattcacg cgccccttgg
     3181 gctggcgcag cg
//
LOCUS       DASPGK010000365         6086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1611862, whole genome shotgun sequence.
ACCESSION   DASPGK010000365 DASPGK010000000
VERSION     DASPGK010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611862"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VEC56_10175"
     CDS             complement(<1..766)
                     /locus_tag="VEC56_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXV14559.1"
                     /translation="MTTYLNMVRTPVGRALVLLVAVTFANATLGVAGAVAQVQAPQTQ
                     TQQQALPADYRVGPGDGIFLSVPQRSDLNRDLVVDANGNVNMPLIGRVLVRGMTDSEI
                     ESRLLQALREYYPSVNRVEVSITRASSNVIWISGEVKLPGKYSFTHPINVWEAIREAG
                     GPNATASLEAVRVIKDRSRGGTTSLVDVSAAMEAGSVDKLPGLDPGDTIIVPARQLEY
                     TGSQGINVMGGVVKPGVYPMQGRQDLMTALLQAGGPS"
     gene            complement(771..1724)
                     /locus_tag="VEC56_10180"
     CDS             complement(771..1724)
                     /locus_tag="VEC56_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV14560.1"
                     /translation="MTTYLVTGGAGFIGSNIVDELVRRGARVRVLDNFSTGRAENLRE
                     AADRIELIEGDIRDEAAVERAVRGVDFVLHQAALASVPRSIADPVSNNQVNVQGTLNV
                     LVAAQRHNVKRVVYASSSSVYGDSEELPKIETMTPNPKSPYAVAKLAAEHYCRVFADL
                     HQMTTVSLRYFNVFGPRQDPTSQYSAVIPIFVRCLLQGQAPTIYGDGEQSRDLTYIEN
                     VVRANLLACEANVAGGRVYNIACGGRYTINQVFQALEKRIGSGLSPRYQAPRAGDVKH
                     SMADISRIRRDLGFEPSVGFEAGIDATVRWYQDAGLETLGK"
     gene            complement(1743..3044)
                     /locus_tag="VEC56_10185"
     CDS             complement(1743..3044)
                     /locus_tag="VEC56_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXV14561.1"
                     /translation="MSLIERIRARDARVAVIGLGYVGLPLAVELARAGFHAVGIDVVE
                     EKVRRVNAGDSYIKDIPSEVLAECVTSGRLTATSDFSVLATCDTVNICVPTPLRKTRD
                     PDISYIVSAVEEIKKYIHKDMLVVLESTTYPGTTEEVILPMLERDDFKVGRDFFLAFS
                     PERVDPGNPRFNTKNIPKVVGGITPACTDVASALYASSLTTVVPVSSAKVAETVKLLE
                     NTFRSVNIGLVNEIALMCNKMDIDVWEVIRAAATKPFGFMPFYPGPGLGGHCIPIDHF
                     YLSWKAKLAGFEARFIELAGQINGSMPEYVVSRVAEALNRRKKALNGSRVLVVGVSYK
                     ANIDDIRESPSLDVMRLLKRAGAEVVYHDRFANADDPELAHAEAESIELTPKALATCD
                     CAVIVTGHEGVDYKALLASVPVVVDTRNVYAGVASEKIVRL"
     gene            complement(3138..4181)
                     /locus_tag="VEC56_10190"
     CDS             complement(3138..4181)
                     /locus_tag="VEC56_10190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXV14562.1"
                     /translation="MKKIIRHRDVLFSTLSEITSLIVAGHDKRMIFRRVLDCASRVLD
                     AEKVHLFELEGNQIVRYTRDQQSGDAEPKVELLQETAGRLGWMIKETESDPFTPGREL
                     GLDVPMLAESLGDETANRVIISAPLVAKRSMFGLLIAIHRDGAMYRPDDIQLLTLLAN
                     QAAIAVENALLYQKLEKEAITDALTQVYNYRFLMSSLEGEIKRARRFKQTFSFVMLDV
                     DNLKSYNDRHGHLSGSQVLREVAGIIKTNCREIDFVSKYGGDEFGVLLPQTKFGGAEK
                     VTQRIIDAVRDHKFDGKRKRLITCSAGISSFPRDGQTAQEIIDAADKALYQAKRTGKN
                     NVVTTESLIEEMA"
     gene            complement(4340..4669)
                     /locus_tag="VEC56_10195"
     CDS             complement(4340..4669)
                     /locus_tag="VEC56_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14563.1"
                     /translation="MFFTPKRRPTAHRLLGRHGRDRRARREHATTLYVTGSIQVALAV
                     ALAAAGFVAFQAVQAHARDVDATTRLALPGAFVIGALVTLRSALRNLRLAKAEAARSN
                     EGDETAR"
     gene            complement(4918..4995)
                     /locus_tag="VEC56_10200"
     tRNA            complement(4918..4995)
                     /locus_tag="VEC56_10200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4958..4960),aa:Arg,seq:ccg)
     gene            complement(4986..>6086)
                     /locus_tag="VEC56_10205"
     CDS             complement(4986..>6086)
                     /locus_tag="VEC56_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14564.1"
                     /translation="SAARVLAHAERIHAAMADACASLYDDEASASAITGRVERHVAPL
                     APLDPRIAEVSELLTQARTALDEAATRARAVIDGLDFEPAEVERLQERLDALTRLEQR
                     YRLDVETLIETARAWKESLAHIEDADQRRGELSREVALRAAAVSETGLALTRTRTRAA
                     AELDRRVTAELSNLHMKGATFRTVVAHEPDPDGAVRVAGAGLVAFEDGLDVVRMRART
                     NPGEAEGGVEAIASTGELSRMALVIKSLTGSGNAGTTLVFDEIDAGVGADLGDVLAEK
                     LLALAARHQILCITHMPQIAAKAPVHIGVAKNVEADRTRVRLARLEGDERTREIARML
                     GGDEGSERRMALARELLAPAPRRTPTSGRVRP"
BASE COUNT          951 a         2009 c         2031 g         1095 t
ORIGIN      
        1 cgctgggtcc cccggcttgc aagagcgcgg tcatcaggtc ctgtcggccc tgcatcgggt
       61 acaccccggg cttcacgacg ccgcccatca cgttgatgcc ctggctgccc gtgtactcca
      121 gctgccgggc cggcacgatg atggtgtcgc ccggatcgag gcccgggagc ttgtcgaccg
      181 agcccgcttc catcgcagcc gacacgtcga ccagcgacgt ggtgcctccc cgcgaacggt
      241 ccttgatcac gcgcaccgct tccagcgaag cggtcgcgtt gggtccgccc gcctcgcgaa
      301 tcgcttccca tacgttgatg ggatgcgtga aggagtactt gcccggcagt ttcacttccc
      361 cgctgatcca gatcacgttg ctcgaggcgc gcgtgatgct cacttcgacc cggttgacgc
      421 tcggatagta ctcgcgcaat gcctgcagca accgcgattc gatctcgctg tcggtcatgc
      481 ctcgaaccag cacgcgcccg atgagcggca tgttcacgtt gccgttggca tcgaccacca
      541 gatcgcggtt caagtcggag cgctgcggta ccgagagaaa tatgccatcg cccggcccga
      601 cgcggtagtc ggcgggcagc gcctgttgct gggtctgggt ctgcggagcc tggacttggg
      661 ccaccgcccc cgcgacgccc agtgtggcgt tcgcgaaggt cactgcgacc agcaggacca
      721 acgcgcggcc gaccggtgtg cgcaccatgt tcaaatacgt cgtcatcgtc ttacttcccc
      781 agcgtctcga gtcccgcatc ctgataccag cgtaccgtcg cgtcgatacc ggcttcgaat
      841 ccgaccgacg gctcgaatcc tagatcacgg cggatgcgag agatgtccgc catcgagtgc
      901 ttgacgtcgc ccgcccgagg cgcttggtag cgcggcgaga gaccggaccc gatgcgcttc
      961 tcgagggcct ggaacacctg gttgatggtg taccgaccgc cgcacgcgat gttgtacacg
     1021 cgcccgcccg ccacgttcgc ctcgcacgcg agcaggttgg cgcgcaccac gttctcgata
     1081 taggtgaggt cgcgcgactg ctcgccgtcg ccgtagatgg tgggggcctg cccctgcagc
     1141 aggcagcgca cgaaaatggg aatcacggcg ctgtactgcg acgtggggtc ctggcgtgga
     1201 ccgaacacgt tgaagtagcg cagcgacacc gtcgtcatct ggtgcaggtc ggcgaagacg
     1261 cggcagtagt gctcggccgc gagcttggcc accgcgtagg gcgacttcgg gttgggcgtc
     1321 atggtctcga tcttgggcag ctcctcgctg tcgccgtaca ccgacgacga cgacgcgtac
     1381 acgacacgct tcacgttgtg gcgctgggcg gcgaccagca cgttgagcgt tccctggacg
     1441 ttgacctggt tgttcgacac cgggtcggcg atcgaacgcg gcaccgacgc gagtgcggcc
     1501 tgatgcagga cgaaatcgac gccgcgcacc gcgcgctcga cggcggcttc gtcgcgtatg
     1561 tcgccctcga tcaactcgat acgatccgcc gcctcgcgca ggttctcggc gcggccggtg
     1621 gagaaattgt ccagcacgcg cacgcgcgcg ccgcggcgca cgagttcgtc gacgatgttc
     1681 gagccgatga agccggcgcc gcccgtcacg agataagtgg tcatggtcgg gtctcccacc
     1741 ggctagaggc gcacgatctt ctcggacgcg acgcccgcgt agacgttgcg cgtgtcgacg
     1801 acgacgggaa cgcttgcgag caacgcctta tagtcgacgc cctcgtgccc ggtgacgatg
     1861 acggcgcaat cgcacgtcgc cagcgccttg ggcgtgagct cgatggactc ggcctcggcg
     1921 tgcgcgagct cggggtcgtc ggcgttggcg aagcggtcgt ggtacacgac ctcggcgccg
     1981 gcgcgcttca acagccgcat gacgtcgagc gagggcgact cgcggatgtc gtcgatgttg
     2041 gccttgtacg agacccccac caccagcacg cgcgagccgt tgagcgcttt cttgcgccgg
     2101 ttgagggcct cggccacgcg gctcacgacg tactcgggca tcgagccgtt gatctggccc
     2161 gcgagctcga tgaagcgcgc ctcgaagccg gccaatttcg ccttccacga caggtaaaag
     2221 tgatcgatcg ggatgcagtg tccacccaac ccggggccgg gatagaacgg catgaatccg
     2281 aagggcttgg tcgccgccgc gcgaatcact tcccacacgt cgatatccat tttgttgcac
     2341 atgagtgcga tctcgttgac caagccgatg ttcacgctgc gaaacgtgtt ctccaagagt
     2401 ttgaccgtct ccgccacttt cgccgagctg accggcacca cggtcgtcaa cgagctcgcg
     2461 tacaaggcac tcgccacgtc ggtgcacgcg ggcgtgatcc cgcccaccac cttcgggatg
     2521 ttcttggtgt tgaagcgcgg gttgcccggg tcgacgcgct cgggcgagaa ggccaggaag
     2581 aagtcgcggc ctactttgaa gtcgtcgcgc tcgagcatgg gcaggatgac ttcctcggtg
     2641 gtcccggggt acgtggttga ctccagcacc accagcatat ccttgtggat gtacttcttg
     2701 atctcctcga ccgcagagac gatgtacgag atgtccgggt cgcgcgtctt gcgcagcggc
     2761 gtgggcacgc agatgttgac cgtgtcgcag gtcgcgagca ccgagaagtc gctggtcgcg
     2821 gtcaggcggc ccgacgtgac gcactccgcc agcacctcgg acggaatgtc cttgatgtag
     2881 ctatcgccgg cattgacgcg tcgcaccttt tcttcgacca cgtcgatgcc gaccgcgtgg
     2941 aagcctgcgc gcgccagctc gaccgccagc ggcaggccga cgtagcccaa tccgatgacg
     3001 gccacccggg cgtcgcgggc gcgaattcgt tcgatgagag acattgcctt cctttccaca
     3061 atgaacgcgg cgagcctata cggacctgcg ctcgagcgac aagtccaatc tggcgcctca
     3121 cgccgcgagc ggcgcgccta cgccatttcc tcgatcaagc tctcggtggt gaccacgttg
     3181 ttcttgccgg tccgcttggc ctggtagagc gccttgtcgg cggcgtcgat gatctcttgc
     3241 gcggtctggc cgtcgcgcgg gaaggacgag atccccgcgc tgcacgtgat gagtcgcttg
     3301 cgtttgccgt cgaacttgtg gtcgcggacg gcgtcgatga tgcgctgcgt gaccttctcc
     3361 gccccgccga acttggtctg cgggagcaac acgccgaact cgtcgccgcc gtacttgctc
     3421 acgaaatcga tctcgcggca gttggtcttg atgatcccgg ccacttcgcg taggacctgg
     3481 ctcccgctca agtggccgtg gcggtcgttg tacgacttca ggttatcgac atcgagcatg
     3541 acgaatgaga acgtctgttt gaagcggcgg gcgcgtttga tctcgccctc cagcgacgac
     3601 atgaggaacc ggtagttgta cacctgggtg agcgcgtcgg tgatggcttc cttttcgagc
     3661 ttctggtaca ggagcgcgtt ctcgacggcg atcgcggcct ggttggcgag caacgtcagg
     3721 agctggatgt cgtcgggccg gtacatggcg ccgtcccggt ggatggcgat cagcaatccg
     3781 aacatcgacc gcttggccac cagcggcgcg ctgatgatga cgcggttcgc ggtctcgtcg
     3841 cccaggctct ccgcgagcat gggcacgtcg agtccgagtt cgcggccggg cgtaaaggga
     3901 tcgctctcgg tttccttgat catccagccc agccggcccg cggtctcctg gaggagttcg
     3961 accttgggct cggcgtcgcc ggactgctgg tcgcgcgtgt accggacgat ctggttaccc
     4021 tcgagttcga agagatggac cttctccgca tcgagcacgc ggctcgcgca gtccaggaca
     4081 cggcggaaga tcatccgctt gtcgtggccg gccacgatca ggctggtgat ctccgacaag
     4141 gtcgagaaca ggacatcgcg atgtcggata atctttttca tcgcaggcta ttacgcgtcg
     4201 tgcctcgtct gatacgtgaa gttcgcttgg cctcggagga accccgtgag tccgtgcaaa
     4261 tcgcatgcca tcggggaggt taacttactg tgaaaacgac tacttaggca atccttgtgc
     4321 gaccgtcgtc ggcgggtgtt cagcgagccg tttcgtcgcc ctcattggag cgggctgcct
     4381 ccgctttcgc gagccggagg ttgcgcagcg cactccgcag cgtgaccaag gccccgatga
     4441 cgaacgcccc cggaagagcc aggcgcgtcg tggcgtcgac gtcccgcgcg tgggcctgga
     4501 cggcttggaa tgccacgaaa ccggccgccg ccaaggcgac cgcgagcgcc acttggatcg
     4561 aacccgtgac gtagagggtg gtcgcgtgct cgcggcgggc gcggcgatcc cgtccgtgac
     4621 ggcccaggag ccggtgggcc gtcgggcgtc gcttgggggt gaaaaacacc ggctaccccg
     4681 acgaccgagg gagatcttcg tagtccgcgt cctcgatcgg atggggcgaa aggggttcgg
     4741 accggggcga attttcccgc ccggcgtctc tgcctgggtg aaacgcctgc gttccgcgcg
     4801 gtcgggggcg ctcggtccgt gcctcacgcg tcgcccgggc caggagacgc gcggccaaga
     4861 gagtgagcag caatcggaat atccagcgga tcacgtcgga cctgactaac ggagaaatgg
     4921 tacgcccggc aggattcgaa cctgcgacac ctggctccgg aggccagtgc tctatccagg
     4981 ctgagctacg ggcgcacgcg acctgacgtc ggggttcgcc gcggcgcggg tgcgaggagc
     5041 tcgcgcgcaa gcgccatgcg ccgctcactc ccctcgtcgc cgcccagcat gcgcgcgatc
     5101 tcgcgcgtgc gttcgtcgcc ttccaagcgc gcgagccgca ctcgtgtgcg gtccgcttcg
     5161 acattcttcg cgacaccgat gtgcaccggc gccttggcgg cgatttgagg catgtgagta
     5221 atacacagta tttgatgccg cgcggcgagc gcaagcagtt tctccgcgag cacgtcgccc
     5281 agatcggcgc cgacacccgc gtcgatctcg tcgaacacga gtgtggtgcc cgcgttcccc
     5341 gagcccgtga gcgacttgat caccagcgcc atgcgcgaca gctctcccgt cgacgcgatc
     5401 gcttccactc cgccttccgc ttcgcccggg ttggtgcgcg cgcgcatgcg cacgacgtcg
     5461 aggccgtctt cgaacgcgac caggccggcg ccggcgacgc gcaccgcgcc gtcggggtcg
     5521 ggctcgtgcg ccaccacggt gcgaaaggtc gcgcccttca tgtgcaggtt cgagagttcc
     5581 gcggtcaccc ggcggtcgag ttccgccgcg gcgcgcgtgc gggtgcgcgt gagcgcgagg
     5641 ccggtctcgg acacggcggc cgcgcgcagc gcgacctccc gcgacaattc accgcggcgt
     5701 tggtccgcgt cctcgatgtg cgcgaggctc tccttccacg cgcgcgcggt ctcgatcagc
     5761 gtctcgacgt cgaggcggta gcgctgctcg agtcgcgtga gcgcgtccaa gcgttcctgc
     5821 aaccgttcca cctcggcggg ctcgaagtcc agcccgtcga tgacggcgcg tgcgcgtgtc
     5881 gcggcttcgt cgagcgcagt gcgcgcctgc gtgagcagtt ccgacacctc ggcgatgcgc
     5941 gggtcgagcg gcgccaaagg cgcgacgtgg cgctcgacgc gccccgtgat cgcggacgcg
     6001 gaggcctcgt cgtcgtagag cgaggcgcac gcgtcggcca tggcggcatg gatcctctcg
     6061 gcgtgggcca gcacgcgcgc ggcgga
//
LOCUS       DASPGK010000366         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_324656, whole genome shotgun sequence.
ACCESSION   DASPGK010000366 DASPGK010000000
VERSION     DASPGK010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324656"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VEC56_10210"
     CDS             <1..832
                     /locus_tag="VEC56_10210"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXV14565.1"
                     /translation="GAGTMGAGIAQVFAASGHFVKLIDVDAEFVKRGLASIDASLDRL
                     LRKETITLATKNEILGRIRTDTSLEATTGSQLVVEAVSEKVDLKKKVWAEVATRVDEQ
                     AILATNTSSISISELGAVVPKPARFIGMHFMNPVPVMKLVEVIRGLETDDSTTTATLD
                     LCRWLGKTPVEVHDYPGFVSNRILMPMINEAVFCLMEGVATQEAIDDVMKLGMAHPMG
                     PLALADLIGLDVCLAIMEVLHRDLGDSKYRPCPLLRKMVAGGRLGRKTGRGFYDYAKA
                     "
     gene            855..1574
                     /locus_tag="VEC56_10215"
     CDS             855..1574
                     /locus_tag="VEC56_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV14566.1"
                     /translation="MNLKLRWIAALLVTMALHACSSDSTPPADVATATAQPPEPVVTH
                     SSADAQPGQRIEHGELILDVDDARKALHAQHHPHGVLETRRVPGVFQPPLMTEVKDFA
                     LSKSDLEVINPGPGEYVHVMEGQRHGYRNLTIGITYTAPGGAPPMHTHMGEESHVLLE
                     GQKILYALGDKMFVMEGPYIINIPPMVPHSFQNLDDEVAELVVIFPTNVWEYDVLDYF
                     PFATPEAQALAQEARGAKPAE"
     gene            complement(1575..>2285)
                     /locus_tag="VEC56_10220"
     CDS             complement(1575..>2285)
                     /locus_tag="VEC56_10220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXV14567.1"
                     /translation="PDEVNARLPLAVLALLGGPSVAGVLLTVVTDGRAGLLELMARLR
                     RGRVATRWYVVALAVAPLLAASLLLALSLHSRVFLPAFLATEDARHLITSGLVIAVGA
                     GVFEEVGWTGFAIPRLRRRHGVLATGLMVGFAWAVWHVLPAYWMSSMVHGPLALTSYV
                     LDPFLFLLPFRVLMVWVYDHTHSVLVAMLMHTSLTATARIVTPTLAGANLIAFDLAWT
                     VAMWVIVAVALRGRARGR"
BASE COUNT          437 a          754 c          762 g          332 t
ORIGIN      
        1 cggcgcgggc accatgggcg ccggtatcgc gcaggtgttc gcggccagcg ggcacttcgt
       61 caagctgatc gacgtcgacg ccgagttcgt caagcgcggg ctcgcgtcga tcgatgccag
      121 cctcgaccgg ctcctgcgca aagaaaccat cacgctcgcg accaagaacg agatcctggg
      181 ccgcattcgc accgacacct cgctcgaggc caccaccggg agtcagctcg tggtggaggc
      241 ggtcagcgag aaagtcgacc tcaagaaaaa ggtttgggcg gaggttgcga cgcgcgtcga
      301 cgagcaggcg attctcgcca ccaacacgtc ttccatttcg atcagcgagc tgggtgcggt
      361 ggtcccgaag cccgcgcgct tcatcggcat gcacttcatg aacccggtcc cggtcatgaa
      421 gctggtggaa gtgattcgcg gcctcgagac cgacgactcc accaccaccg cgaccctcga
      481 cttgtgccgc tggctcggaa agacgccggt cgaagtccac gactaccccg gcttcgtgtc
      541 gaatcgcatc ctgatgccga tgatcaacga ggccgtgttc tgcttgatgg aaggtgtcgc
      601 cacccaggaa gccatcgacg acgtgatgaa gctcggcatg gcgcacccga tgggcccgct
      661 cgcgctggcc gacttgatcg gcctcgacgt gtgcctcgcc atcatggaag tcctgcaccg
      721 cgacctggga gattcgaagt atcgcccgtg tccgctcttg cgcaagatgg tcgcgggcgg
      781 acgcctgggg agaaagacgg gacgcgggtt ctacgactac gcaaaggcgt aggcaacggc
      841 cacaggagga aaccgtgaat ctcaagctcc gttggatcgc cgctctcttg gtaacgatgg
      901 ctctgcacgc gtgctcgtcg gattccacgc cgccggcgga cgtggcgacg gcgaccgccc
      961 aaccgcccga gccggtggtg acgcattcgt cggcggacgc acaaccgggg cagaggatcg
     1021 agcacggcga gttgattctc gacgtcgacg acgcgagaaa agcgttacac gcgcagcacc
     1081 atccccacgg cgtgctcgaa acccggcgcg tgcccggcgt gttccagccg cccctgatga
     1141 ccgaggtcaa ggacttcgcg ctctccaaga gcgatctcga ggtgatcaat cccggtccgg
     1201 gcgagtacgt ccacgtcatg gagggacagc ggcacgggta tcgcaatctg accatcggca
     1261 tcacctacac cgcgcccggg ggcgcgccgc ccatgcacac ccacatgggc gaggaatccc
     1321 acgtgctgct ggaagggcag aagatactct atgcgctggg tgacaagatg ttcgtgatgg
     1381 agggcccgta catcatcaac attccgccca tggtgcccca ttcgttccag aacttggacg
     1441 acgaggtggc cgagttggtg gtgatattcc cgaccaacgt gtgggagtac gacgtgctcg
     1501 actacttccc gttcgcgact cccgaggccc aggccctcgc gcaggaggcc aggggcgcga
     1561 agccggcgga gtagttaccg gccgcgtgcg cgaccgcgga gggcgacggc gacgattacc
     1621 cacatcgcga ccgtccacgc gagatcgaac gcgatcaggt tcgcgcccgc gagcgtcggc
     1681 gtgacgatgc gcgcggtggc ggtgaggctc gtgtgcatca gcatcgccac gagcacgctg
     1741 tgggtgtggt cgtacaccca caccatcagc acgcggaacg gcagcaagaa caagaacggg
     1801 tcgagcacgt agctggtgag cgcgagcggc ccgtgcacca tgctgctcat ccagtaggcc
     1861 ggcagcacgt gccacacggc ccacgcgaac cccaccatca gcccggtcgc gagtacgccg
     1921 tgccgtcgcc gtagtcgcgg gatcgcgaag cccgtccagc ccacctcttc gaaaaccccg
     1981 gccccgaccg cgatcacgag gcctgacgtg atcaggtgac gcgcgtcctc ggtggcgagg
     2041 aacgcaggga ggaacacgcg cgaatgaagc gagagcgcga gcaggagcga cgccgcgagg
     2101 agcggcgcga ccgccagcgc gacgacgtac cagcgggtgg ccacgcgccc gcggcgcagg
     2161 cgcgccatga gttcaaggag tcccgcccgg ccgtcggtga ccacggtgag gagaacgccc
     2221 gcgacgctgg ggccgccgag caacgcgagt accgcgagcg gcaaccgcgc gttgacttcg
     2281 tcggg
//
LOCUS       DASPGK010000367         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1259555, whole genome shotgun sequence.
ACCESSION   DASPGK010000367 DASPGK010000000
VERSION     DASPGK010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259555"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VEC56_10225"
     CDS             complement(<1..404)
                     /locus_tag="VEC56_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HXV14568.1"
                     /translation="MGEVALYFSGLVVLLLLSAFFSGSEAALFSLSRSQQRVLRDASP
                     GGRVAVRLLAHPRPLLITVLVGNLAVNVLATSTATALAIDAFGARGVGLAFAVMSMLI
                     MVLTEIVPKALGMHWAPRVAPVVALPLSIIHVL"
     gene            complement(421..1185)
                     /locus_tag="VEC56_10230"
     CDS             complement(421..1185)
                     /locus_tag="VEC56_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14569.1"
                     /translation="MRRIAFVLVVAVGSAALSGCWGAKWAKGPINAEKASLKADTLRA
                     EQRRMAEQLDRVERQLAAESEERTASQARLASTLSDVEEMLRILSSRIEDNEQMQLNR
                     GGRDRSSGSRPSVGSAPAPTDSAALAAQAAEDLYRAAYLDVTRGNYDLAAPAFRDYLA
                     KNPSGARAAEAHYYLGECEYATERWLEAAGQFQQVVRDYPKARLVPAAYLKMGHSYTQ
                     LEERGLAGQAYRALIEKHPNSEEAKQARAALNELGG"
     gene            complement(1205..1711)
                     /gene="pal"
                     /locus_tag="VEC56_10235"
     CDS             complement(1205..1711)
                     /gene="pal"
                     /locus_tag="VEC56_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXV14570.1"
                     /translation="MIKRLMVLAAILSLVAVAGCGGKKAVEPTPTDTGSSTPVEEPQE
                     APVEETSSGNTDMSPISLSDIFYDFDQYNLTSEAKGTLEANARELKRVGDAAITIEGH
                     CDERGTKAYNLALGDKRANAAKAYLVSLGVTSSRITTISYGKERPFDTGHSESAWAKN
                     RRAHFVTK"
     gene            complement(1793..>2182)
                     /locus_tag="VEC56_10240"
     CDS             complement(1793..>2182)
                     /locus_tag="VEC56_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14571.1"
                     /translation="PGGREIVFLSDRSGSPQLYLMDRDGSNVRRLTFTGGYNASPAWS
                     PRGDLIAFSTRMGAEFQIAVITPDGRDGRLITKDNHSHEDPRWAPDGRHLVCTERGSG
                     ESVSVVDIVTGGKRILAQGSDPDWSTP"
BASE COUNT          369 a          713 c          743 g          357 t
ORIGIN      
        1 agaacgtgga tgatcgagag cgggagcgcc accacgggcg ccacgcgcgg ggcccagtgc
       61 atcccgagcg ccttggggac gatctcggtg agtaccatga tgagcatcga catgacggcg
      121 aacgcgagcc ccaccccgcg cgcaccgaag gcgtcgatgg cgagcgcggt ggcggtgctg
      181 gtggccagca cgttgacggc gagattgccg accaggaccg ttatgagaag cgggcgcggg
      241 tgcgcgagca ggcggacggc gacgcgaccg ccgggtgaag cgtcgcggag cacgcgctgc
      301 tggctgcgcg aaagcgagaa gagagcggct tcggaccccg aaaagaaggc ggagaggagc
      361 aggagtacga cgagaccgga aaagtagagt gcgacttcgc ccacggcggc gaacgaccgg
      421 ctatccgccg agctcgttca gggcggcgcg ggcctgtttg gcttcttcgg agttggggtg
      481 cttctcgatg agcgcgcggt acgcctgccc ggcgagcccc cgctcttcga gctgcgtgta
      541 cgagtgcccc atcttgagat acgcggcggg caccaggcgt gccttggggt agtcgcgcac
      601 cacctgctgg aactgccccg cggcctcgag ccagcgctcg gtcgcgtatt cgcactcgcc
      661 caggtagtag tgggcctcgg cggcacgcgc gcccgacggg ttcttggcca agtagtcccg
      721 gaacgcgggc gcggccaggt cgtagttgcc gcgcgtcacg tcgaggtacg cggcgcgata
      781 gaggtcctcg gcggcttgcg cggctaacgc cgccgagtcg gtcggcgcag gggcggagcc
      841 gacggaggga cgcgatccgc tcgagcgatc gcgccccccg cggttcaact gcatctgctc
      901 gttgtcctcg atgcggctcg agagtatgcg cagcatttcc tcgacatcgc tgagcgtgga
      961 cgccaggcgc gcctgcgacg cggtgcgctc ttccgactcg gcggccaact gccgctcgac
     1021 ccggtcgagt tgctcggcca ttcgccgctg ctcggcccga agcgtgtcgg ccttgaggct
     1081 cgccttctcg gcattgatag gccccttggc ccacttcgcg ccccaacagc ccgacagagc
     1141 cgccgaacca accgccacta ccaacacgaa cgcgatgcga cgcacgtcga ggactcctcc
     1201 cgagctactt cgtcacgaag tgcgcgcgac gattcttcgc ccaggcgctt tcgctgtgcc
     1261 ccgtgtcgaa cgggcgctcc ttgccgtagc tgatggtcgt gatgcgagag gacgtgacac
     1321 cgagcgagac caagtaggcc ttggccgcgt tggcgcgctt gtcgccgagg gcgaggttgt
     1381 acgccttggt accgcgctcg tcgcagtgac cctcgatcgt gatggccgcg tcacccacgc
     1441 gcttgagctc gcgcgcattc gcctccaaag tccccttggc ctccgacgtc aggttgtact
     1501 ggtcgaaatc gtagaaaatg tcgctgagcg agatcggcga catgtcggta ttgcctgacg
     1561 acgtttcttc gaccggggct tcttgcggct cctcgaccgg cgtcgacgag ccggtatccg
     1621 tcggggtggg ctcgaccgcc ttctttccgc cgcaacccgc gaccgccacg agcgacagaa
     1681 tcgccgcgag caccatcaat cgtttgatca tcccatgacc tcctggaaag ccgatgtgcg
     1741 tgacaagttg aaaaagtggc gtccggggca agacgcgcta agtgtaatca aattaagggg
     1801 tcgaccaatc ggggtcggaa ccctgggcca atattctctt accccccgta acaatgtcaa
     1861 ccacggaaac cgactcgccg ctgccgcgct cggtacagac gagatggcgg ccgtcgggcg
     1921 cccacctcgg gtcctcgtgg ctgtgattgt ccttggtaat gagccgtcca tcacgcccgt
     1981 ccggggtgat gaccgcgatc tggaactcgg cgcccatgcg ggtcgagaat gcgatgaggt
     2041 cgccacgcgg cgaccacgcg ggcgaggcgt tgtagccgcc cgtgaacgtc aagcgacgca
     2101 cgttgctgcc gtcgcggtcc atcaagtaga gctgcggcga acccgagcgg tcggagagga
     2161 acacgatctc gcgcccgccc gg
//
LOCUS       DASPGK010000368         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_206260, whole genome shotgun sequence.
ACCESSION   DASPGK010000368 DASPGK010000000
VERSION     DASPGK010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206260"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VEC56_10245"
     CDS             <1..478
                     /locus_tag="VEC56_10245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV14572.1"
                     /translation="IDNFKGFNDYYGYQKGDDIIEFMADVLRRTVVKLGGNDDFVGHI
                     GGDDFVMVVPPSRAEIMAQYVVDEFDKGTLLLLDQEDVKRGYLEVRDRQGELKRIPVM
                     SVTIALVKSTDNQIEHFAEVNDIASSLKEYGKRIEGSVVVKERRQDGATKNTPTRK"
     gene            488..1321
                     /locus_tag="VEC56_10250"
     CDS             488..1321
                     /locus_tag="VEC56_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV14573.1"
                     /translation="MDIRTAEDKTAVLEALLHEARIEANLLRQRLEFYRRVIASTRLV
                     MGHEFKKPTTAISGYLELAHEDVEKAGLSAALAFIEKARVECALLNELNAFYLDLLKV
                     DGGSGEPAAESVDMLETIVDAIEQTPERLRAKERVRIVVADRFPRVRLNRYAVKLIVL
                     NLLENALKYSPEGSAVRVEAETSMDKRGAGDRELLKLRVIDYGAGIAPDDVKRIFMPF
                     VRLDASQIEGSGLGLTLVRNLVDMCEGEVSIRSDPGKGTTVFVTLPFTKSGGSAGVVR
                     P"
     gene            1318..>2056
                     /locus_tag="VEC56_10255"
     CDS             1318..>2056
                     /locus_tag="VEC56_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14574.1"
                     /translation="MKSASGWGPDTRALESSPLSILYLRFIELFAFFGIVFAARFLGD
                     LAYQTNVLVILFLAAAFMQYSGEWLWGAVGSRLGWDKKRRNIALLWYSSYVDLAAVIG
                     LIYLTGTVESPFLFLLTIPLFFVCNTFSWKVTTAFFVSAALIALGVIGHLEMQGTIPH
                     YTVYNASPGVYMNKHYLAGSLLVIGAFVSLVIYLSNAFQDRMSTSLERMRKKREESES
                     RANELSRLYDISTGINAAISLETLLKIV"
BASE COUNT          383 a          636 c          669 g          368 t
ORIGIN      
        1 catcgacaac ttcaagggct tcaacgatta ctatggctac caaaagggcg acgacatcat
       61 cgagttcatg gccgacgtgc tgcgccgcac ggtggtcaag ctgggcggca acgacgactt
      121 cgtgggccac atcggcggcg acgacttcgt gatggtggtt ccgccctcgc gcgccgagat
      181 catggcgcag tacgtcgtcg acgagttcga caagggcacg ctgctcctgc tcgaccaaga
      241 agacgtcaaa cgcggctacc tcgaggtgcg cgaccgccag ggcgagctca agcgcattcc
      301 cgtcatgtcg gtcaccatcg cgctggtcaa gagcacggac aaccagatcg agcacttcgc
      361 ggaagtgaac gacatcgctt ccagcctgaa ggaatacggg aaacgaatcg aaggcagcgt
      421 cgtcgtcaag gagcgccgcc aagacggcgc gaccaagaac acgccgacgc gcaagtaaga
      481 ggggaacatg gacattcgta ccgccgaaga caagaccgcc gtactcgaag cgctcctgca
      541 cgaagcgcgc atcgaagcga acctgcttcg ccagcgcttg gagttctacc ggcgcgtgat
      601 cgcgtcgacg cgtctggtca tgggccacga gttcaagaag cccaccaccg ccatcagcgg
      661 ctacctcgaa ctcgcgcacg aggacgtgga gaaggcgggc ttgagcgccg cgctggcttt
      721 catcgagaaa gcgcgcgtcg aatgcgcgct gctgaacgag ctcaacgcgt tctacctgga
      781 cctgctcaag gtggacggcg gcagcggaga gccggccgcc gagtcggtcg acatgctgga
      841 gaccatcgtc gacgccatcg agcagacgcc cgagcgcctg cgcgccaagg agcgcgtgcg
      901 catcgtggtc gctgatcgct tcccgcgcgt gcgcctgaat cgctacgcgg tgaagctgat
      961 cgtgctgaac ttgctggaga acgcgctcaa gtactcgccc gagggttccg cggttcgtgt
     1021 cgaggcggag acgtcgatgg acaagcgcgg cgcgggcgac cgcgagctgc tgaagctgcg
     1081 cgtcatcgac tatggcgccg gcatcgcgcc cgacgacgtg aagcggatct tcatgccgtt
     1141 cgtgcgcttg gacgcgagcc agatcgaggg tagcggcctg gggctgacgc tggtgcgaaa
     1201 cctggtcgac atgtgcgagg gcgaagtgtc gattcgtagc gatcccggca agggcacgac
     1261 cgtcttcgtg accttgcctt tcaccaagtc cggcgggagc gccggggtgg tgcgcccgtg
     1321 aaaagcgcgt cgggctgggg cccggacacg cgcgccttgg agtcgagtcc gttgagcatc
     1381 ttgtacttga gattcatcga gctgttcgcg ttcttcggga tcgtgttcgc cgcgcgtttc
     1441 ctcggcgacc tcgcgtacca gacgaacgtg ctggtgattc tgttcctggc cgccgccttc
     1501 atgcaataca gcggcgagtg gctgtggggc gcggtcggat cgcgcctggg ctgggacaag
     1561 aagcggcgta acatcgcgct cctttggtac tcgagctacg tcgacctcgc ggccgtgatc
     1621 gggctcatct acctcaccgg gaccgtcgag agtccgttcc tgttcctgtt gacgattccg
     1681 ctcttcttcg tgtgcaacac gttttcgtgg aaggtcacga ccgccttctt cgtgtcggcg
     1741 gctctgatcg cgctgggcgt gatcggacac ctcgagatgc agggcaccat cccgcactac
     1801 acggtctaca acgcgtcgcc cggcgtgtac atgaacaagc actacctggc tggctcgctg
     1861 ctggtgatcg gcgcgttcgt gagcctggtc atctatctgt cgaacgcctt ccaggatcgc
     1921 atgagcacct cgctcgagcg catgcggaag aagcgtgaag agtcggagtc gcgcgccaac
     1981 gagctgtcgc gcctgtatga catcagcacc ggcatcaatg ccgccatctc gctggagacg
     2041 ctcctcaaga tcgtgg
//
LOCUS       DASPGK010000369         6691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1729820, whole genome shotgun sequence.
ACCESSION   DASPGK010000369 DASPGK010000000
VERSION     DASPGK010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729820"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VEC56_10260"
     CDS             <1..1182
                     /locus_tag="VEC56_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14575.1"
                     /translation="MLQYDMPERADGRFHAGAEYAFSKFLALRAGFDDDTATFGLGVG
                     AFDYGLDMAYYSKDEAGSTQAYAFTGAWGNTLEEKREEIARTRAEEERLLIQKTFDSR
                     VQALRKRAQEMEAAGDYSGALAQWQVVLEFVPDDAQAVAAASEARERLLAEQAASVRD
                     VENQAVIRTRFAEGLDRYDQSDFLGARTEWRAILAIDSTHAGAQEYLEKTNLKIDEIV
                     RGHVSRARNYENGGRLTEAIAEWNNVQQYRPDDPQARAAITRIRGRIESVSQDYSATQ
                     RKLRIVTLYNDALTAYNEGRYPDTMTGLRELLQLQPDHADAKKLMTLAKRRTTPLSDA
                     EKARIRELYLAGMQHFSKDNYAQAIAEWQKILEIDPTNESVARSIEEAQERLRRTQER
                     R"
     gene            1184..5440
                     /locus_tag="VEC56_10265"
     CDS             1184..5440
                     /locus_tag="VEC56_10265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HXV14576.1"
                     /translation="MARPALVERNERRELTVLAQEGQLGRVRDFVVAVCEDAGFSPRE
                     IANTKLAVDEACTNIIKHAYEGKTETGDIGVVADIEAGRVRIHLRDRGKHFDFASVKD
                     PDLDQYVETGKKGGLGVFLINRLMDGVEYRTTEVGNELVLTKRSQAAFSAAVLPEKMP
                     LRGTLRFKFMVRASGGLAALMLVAWAFIMVRQTSDIDGQRTTQWMEKRRLAESIASRS
                     VSTLVDPSEFSIDQTNLTSELAKLVGAGDEIAYARVVDAMGNVRASGRVEEVFQPDPG
                     PAGEIISEDGAVTWSRHDSEQGEIRDIAVRVAVPGADGQRFDVGVMHLGVYKDAVEGA
                     IEDTRLSTTLLLLGIFAVGILLIMGLVQVFVRPIQLLTDGVRAIGDGNMDSKLDVRGP
                     AEIGAIAGVFNEITEKFKKARDSILEQEKMQKEIEVAKQIQQSLLPRRRPEISGYDIA
                     PLYQAAAEVGGDYYDFVQVDDDTIGVVVADVSGKGVPGSLVMTMIRTALRMEARGNRN
                     ASDVMSKMNTFVTDDMKKGMFVTMFYVILDSKNRVISYASAGHNPMILYRHETRETFF
                     LNPRGFPVGISLPDETLFRRSISLEKVRLKKDDMLVIYTDGVTEAMNEKREQYGEERL
                     IQIVKTHGHLSPDDFISRLDAELKGFTAGHPQSDDITVVAIKERMTADDILFGIRRKL
                     IDLVDVQGMNVKDACRQMKVSPATYYRYKKRLEVMGERGLRNDILRDDVSIKRVSLEA
                     RKEIVRIIAADPDLGAKRITEEYNKGRDASLHVTERMVYEELKRLNMNTRELRLDYLR
                     RHGLVRDADGALPAAKETRGQPAARDLVDDLLREIPGVLPPASVPDASAAGAGAEPPP
                     VQYQVEGVGVSVSTSDGITDVRLSGHVDSASSAGLERRLRDLTAAGAAKIVVDLTDVS
                     YVSSGGWGIFVGEVGGLRKRGGDIVLSGMTPEVFDVYELLGFADVLRSFGTVTEARAW
                     LSLPPDARGAAVVARPATPEHDLRAGVDFEDGAMEPVDSEAAREWQSLRIEATTVGEK
                     GDVAVLALAGIIDTVSAERLREALHQVIAGGRMKIVADMSHVEYVSSGGWGVFTERLR
                     EVRRAGGDIKLFGMDPDVYYVFTMLGFNIVLSSFDLFADAIDDFRRTAPGMEAPSAEP
                     TKPARLDEPTEAVRSVWSLAATPGLDLSWEDGPDGIRIARLAGVIETTAVSLIADELG
                     RELERAPRAFVFDLAKVEYISSSGWGQFARAFEATRSVALVGMGPDLLEVYDCLEFRN
                     FIPAYATQTEALRTLAGGGPEPTTAPSSEMPGVSEPRPSKRAAGKAVAPTHDDGLDDV
                     LGSPSPPRVDDAGGREVPKGFDAIAPDETPLPRGSEWKDAGTSPDVSIDSAVSDRNKD
                     RDKKLRSLGWQRYGERLRRGDDTADPGAGRKPDDGSDDEH"
     gene            5461..6198
                     /locus_tag="VEC56_10270"
     CDS             5461..6198
                     /locus_tag="VEC56_10270"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXV14577.1"
                     /translation="MKKIAIITSKGGTGKTTTAINLGHGLALCGMKVLIVDCDPQRNV
                     AIAFDVKGERTLCNLLQFGEVDVIEARQNLFVIDSGGRDLAEIEMVLASRSSRERRLE
                     MALKNLRGCDVVICDCSPTVNLININAIVYADEVIIPVSMDYLAQAGARQTMQIIDEI
                     NAHTERKTSILGILPTFYDSRTRLSTDVLEALRERFQDKVFKTVIRVNTSLREAPSFN
                     QTIFEYSPMSRGAFDYYQLTEEVLSRA"
     gene            6191..6463
                     /locus_tag="VEC56_10275"
     CDS             6191..6463
                     /locus_tag="VEC56_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14578.1"
                     /translation="MPDRRKKSIGRDPFEDAKEDRESRTVERLIRGKVALAREAKEIT
                     VGVKLTPSSLKHLDKVRDRLRARGREVSRDELIRIAITLLTEDDVP"
BASE COUNT         1207 a         2191 c         2306 g          987 t
ORIGIN      
        1 atgctccagt acgacatgcc cgagcgcgcc gacgggcgct tccacgcggg cgccgagtac
       61 gcgttctcga agttcctggc tttgcgcgcg ggcttcgacg acgacaccgc gaccttcgga
      121 ctgggcgtgg gtgcgttcga ctacggcctc gacatggcct actactcgaa ggacgaagcg
      181 ggttcgaccc aggcctatgc cttcaccggt gcctggggca ataccctgga ggagaagcgc
      241 gaagagatcg cgcgcacgcg cgcagaagag gaacggctac tcatccaaaa gaccttcgac
      301 tcgcgcgtgc aggcgttgcg caagcgcgcg caggaaatgg aagccgcggg cgactattcg
      361 ggcgccctcg cgcagtggca ggtcgtgctc gagttcgtcc ccgacgacgc ccaagcggtg
      421 gcggcggcct cggaggcgcg cgagcgcctc ttggccgagc aagccgcctc ggtgcgcgac
      481 gtcgagaacc aggccgtgat tcgcacgcgc ttcgcggagg gactcgaccg ctacgaccag
      541 agtgacttcc tgggcgcgcg caccgagtgg cgcgcgattc tcgcgatcga ttcgacccac
      601 gcgggcgcgc aggagtacct cgaaaagacc aacctcaaga tcgacgagat cgtgcgcggc
      661 cacgtatcgc gcgcgcgcaa ctacgagaac ggcgggcgcc tcaccgaagc gatcgccgag
      721 tggaacaacg tccagcagta ccgccccgac gatccccagg cgcgtgccgc catcacgcgc
      781 atccgggggc gcatcgagtc ggtgtcgcag gactacagcg ctacccagcg caagctccgc
      841 atcgtgacgc tctacaacga cgccctcacg gcgtacaacg aggggcgtta cccggacacg
      901 atgacgggcc tgcgcgagct cttgcagctc cagcccgacc acgcggacgc gaagaagctc
      961 atgacgctgg ccaagcgccg cactacaccg ctctccgacg ccgagaaggc gcgcatccgc
     1021 gagctctacc tggcgggtat gcagcacttc tcgaaggaca actacgcgca ggccatcgcg
     1081 gagtggcaga agatcctcga gatcgacccc accaacgaga gtgtcgctcg cagcatcgag
     1141 gaggcgcagg agcgcctgcg ccgcacgcag gagcgccgct agtatggcgc gcccggcctt
     1201 ggtcgagagg aacgagcggc gcgagctcac ggtgctggcg caggaaggcc agctcgggcg
     1261 ggtgcgcgac ttcgtggtcg cggtgtgcga ggacgccggt ttcagcccgc gcgagatcgc
     1321 caacaccaag ctcgcggtcg acgaagcctg cacgaacatc atcaaacacg cctacgaggg
     1381 gaagaccgag accggcgaca tcggcgtggt ggccgacatc gaagccgggc gcgtgcgcat
     1441 ccacctgcgc gaccgcggca agcacttcga cttcgcgagt gtcaaggacc ccgacctcga
     1501 ccagtacgtc gaaaccggca agaagggcgg cctcggcgtc ttcctcatca atcgcctgat
     1561 ggacggggtc gagtaccgca ccaccgaggt cggcaacgag ctcgtgctga ccaagcgcag
     1621 ccaggccgcc ttctcggccg cggtgctgcc cgagaagatg ccgctgcgcg gcacgcttcg
     1681 tttcaagttc atggtgcgcg cgagcggcgg tctggcggca ctgatgctgg tggcctgggc
     1741 cttcatcatg gtgcgccaga ccagcgacat cgacgggcag cgcaccacgc agtggatgga
     1801 gaagcgccgg ctggcggaga gcatcgcgag ccgcagcgtg tccacgctgg tcgaccccag
     1861 cgagttctcg atcgatcaga ccaacctgac ctccgagctg gccaagctgg tgggcgcggg
     1921 cgacgagatc gcctacgcgc gcgtggtgga cgcgatgggc aacgtgcgcg cgtcgggtcg
     1981 ggtcgaagaa gtcttccagc ccgaccccgg cccggccggc gagatcatct ccgaggacgg
     2041 cgcggtgacg tggtcgcgcc acgactccga gcagggcgag attcgcgaca tcgcggtgcg
     2101 ggtagccgtc cccggcgccg acggccagcg cttcgacgtg ggcgtgatgc acctgggtgt
     2161 ttacaaggac gcggtcgagg gcgcgatcga ggacacgcgc ctgtcgacga ccctcttgct
     2221 cttgggcatc ttcgcggtcg gcatcctcct catcatgggg ctggtgcagg tcttcgtgcg
     2281 gcccattcag ctgctcaccg acggcgtgcg cgcgatcggc gacggcaaca tggacagcaa
     2341 gctcgacgtg cgcggaccgg ccgagatcgg cgccatcgcg ggcgtgttca acgagatcac
     2401 cgagaagttc aagaaggcgc gcgactcgat cctcgagcag gagaagatgc agaaggagat
     2461 cgaggtcgcc aagcagatcc agcagtcgct cctgccgcgc cggcgcccgg agatcagcgg
     2521 ctacgacatc gcgccgctgt atcaagcggc cgcggaggtg ggcggcgact actacgactt
     2581 cgtccaggtc gacgacgaca ccatcggcgt ggtggtggcc gacgtgtcgg gcaagggggt
     2641 ccccgggtcg ctggtcatga ccatgattcg caccgcgctg cgcatggagg cgcgcgggaa
     2701 ccgcaacgcc tccgacgtca tgtccaagat gaacacgttc gtgaccgacg acatgaagaa
     2761 gggcatgttc gtcaccatgt tctacgtgat cctggactcc aagaaccgcg tcatctcgta
     2821 cgcgagcgcg ggacacaacc cgatgatcct ttatcgccac gagacgcgcg agacgttctt
     2881 tctgaacccg cgcggtttcc cggtcgggat cagcctcccc gacgagacgc tgttccgccg
     2941 ttccatcagc ctggagaaag tccgcctcaa gaaggacgac atgctggtga tctacaccga
     3001 cggcgtcacc gaagccatga acgagaagcg cgagcagtac ggcgaagagc ggctgattca
     3061 gatcgtgaaa acgcacggcc atctttcgcc cgacgacttc atatcgcggc tcgacgccga
     3121 gctcaagggg ttcaccgccg gccatcccca gagcgacgac atcaccgtgg tggcgatcaa
     3181 ggagcgcatg accgccgacg acatcctgtt cggcattcgc aggaagctga tcgacctggt
     3241 ggacgtgcaa ggcatgaacg tcaaagacgc atgccggcag atgaaggtgt cgcccgcgac
     3301 gtactatcgc tacaagaagc ggctcgaagt gatgggcgag cgggggctgc gcaacgacat
     3361 cctgcgcgac gacgtgagca tcaagcgcgt gagcctggag gcgcgcaagg agatcgtgcg
     3421 catcatcgcg gccgacccgg atctgggagc caagcgcatc accgaggagt acaacaaggg
     3481 ccgcgacgcg tcgctccacg tcaccgagcg catggtgtac gaggagctca agcgcctcaa
     3541 catgaatacc agagagctcc gcctcgacta cctgcgccgg cacgggctgg tgcgagacgc
     3601 cgacggcgcg ctcccggccg ccaaggagac gcgtgggcaa ccggctgcac gcgacctggt
     3661 cgacgacctc ttgcgcgaga tccccggcgt cctgccgccg gcgtcggtcc ccgacgcgag
     3721 tgccgcgggc gcgggcgcgg aaccgccccc cgttcagtat caggtcgaag gcgtcggcgt
     3781 ctcggtgtcg acgtcggacg gtatcaccga cgtgcgcctc tccggccacg tcgactcggc
     3841 ctcgtcggcc gggctggagc gccggctgcg cgacctcacc gcggccgggg cggccaagat
     3901 cgtcgtcgac ctgaccgacg tgtcgtacgt ctcgagcggt ggctggggga ttttcgtggg
     3961 tgaagtgggc gggttgcgca agcgcggcgg cgacatcgtg ctctccggca tgacccccga
     4021 agtgttcgac gtgtacgagc tcttgggttt tgccgacgtg ctgcgctcgt tcggcaccgt
     4081 gaccgaggcg cgcgcgtggc tctcgctccc gcccgacgcg cgcggggccg ccgtggtcgc
     4141 gcgtcccgcg acccccgagc acgacctgcg cgcgggcgtg gatttcgaag acggagcgat
     4201 ggagccggtg gattcggaag cggcgcggga gtggcagagc ctgcgcatcg aggcgaccac
     4261 ggtgggcgag aagggcgacg tcgccgtgct ggcgctggcc gggatcatcg acacggtgag
     4321 cgcggaacgc ctgcgcgagg cgctgcacca ggtgattgcg ggcggccgca tgaagatcgt
     4381 cgccgacatg tcgcacgtcg agtatgtgtc cagcggcggt tggggcgtgt tcaccgagcg
     4441 cttgcgcgaa gtgcgccggg cgggcggcga catcaagcta ttcgggatgg accccgacgt
     4501 gtactacgtg ttcacgatgc tgggcttcaa catcgtgctg tcgtcgttcg atctgttcgc
     4561 ggacgcgatc gacgattttc ggcgcacggc gccgggtatg gaggccccgt ccgcggaacc
     4621 gaccaagccg gcgcgcctcg acgagcccac cgaagcggtg cgctcggtgt ggtcgctggc
     4681 cgcgacgccg gggctcgacc tctcgtggga agacggcccc gacggcatcc gcatcgcgcg
     4741 cctcgccggc gtgatcgaga ccactgcggt ctcgctcatc gccgacgaac tcgggcgcga
     4801 gctcgagcga gcaccgcggg cgttcgtgtt cgacctggcg aaggtcgaat acatatcgag
     4861 cagcggctgg ggtcagttcg cgcgcgcctt cgaggccacg cgctcggtgg cgctggtggg
     4921 gatggggccc gacttgctcg aggtatacga ttgtctggag tttcgcaatt tcattcccgc
     4981 gtacgcgacc cagacggagg ccctgcgaac cctcgccggc ggcggcccgg aaccgacgac
     5041 ggcgccgtcg tcggagatgc cgggtgtgag cgagccgcgt ccgtcgaaac gcgcggccgg
     5101 gaaagcggtc gcgcccacgc acgacgacgg attggacgac gtgctgggat cgccgtcgcc
     5161 gccccgcgtc gacgatgcgg gcgggcgcga ggtacccaag gggttcgacg ccatcgcgcc
     5221 cgacgagaca ccgctgccgc gcgggtccga gtggaaagac gcgggtactt cgcccgacgt
     5281 cagcatcgac tcggcggtct cggaccgcaa caaggaccgc gataagaagc tgcgctcgct
     5341 gggctggcag cgttacggcg agcgcctgcg tcgcggcgac gacaccgccg acccgggcgc
     5401 gggccggaaa cccgacgacg ggagcgacga cgagcactag actcgcgtcg ccgcggcgcc
     5461 atgaagaaga tcgcgatcat cacatcgaag ggcggcaccg gaaaaaccac caccgccatc
     5521 aacctcggcc acggcctcgc gctgtgcggc atgaaggtgc tgatcgtcga ctgcgacccc
     5581 cagcgcaacg tggccatcgc cttcgacgtc aagggcgagc gcacgctgtg caacctgctc
     5641 cagttcggcg aggtggacgt gatcgaagcg cggcagaacc tattcgtgat cgattcgggc
     5701 gggcgcgacc tggccgagat cgagatggtg ctggcgtccc gctcgagccg cgagcggcgc
     5761 ctcgagatgg ccttgaagaa cctgcgcggc tgcgacgtcg tcatctgcga ctgctcgccg
     5821 accgtcaact tgatcaacat caacgcgatc gtctacgccg acgaggtcat cattcccgtt
     5881 agtatggact acctggccca ggcgggcgcg cgccagacca tgcagatcat cgacgagatc
     5941 aacgcgcaca cggagcgaaa gacgtcgatc ctcggcatcc tgccgacgtt ctacgacagc
     6001 cgcacgcgcc tctccaccga cgtgttggag gcgctgcggg agcggttcca ggacaaggtg
     6061 ttcaagaccg tgattcgcgt caacacgtcg ctgcgcgagg cgcccagctt caaccagacc
     6121 atcttcgagt attcgcccat gtcgcggggt gcgttcgact attatcaatt gaccgaagag
     6181 gtgctgtccc gtgcctgacc ggcgcaagaa atcgattggg cgcgatccct tcgaggacgc
     6241 caaggaagac cgcgagtcgc gcacggtcga gcgcttgatt cgcggcaagg tggcgctcgc
     6301 gcgcgaggcc aaggagatca cggtcggggt caagctgacg ccgtcgagcc tgaagcacct
     6361 cgacaaggtt cgcgaccgcc tgcgcgcgcg cgggcgcgag gtgtcgcgcg acgaactgat
     6421 tcgcatcgcg atcacgctcc tgaccgagga cgatgtgccg tgagtcgcga cgcttgtccg
     6481 atggaccggg gcgcgctata gtacaggatt gcgcatgagt gaggttgtca cgatggaagg
     6541 ctacgaccac cgtagcgaga tcgtatcgag cgggacgcgc tactacgtgc agtccagcgt
     6601 ggtgccggcg cagcgcacgg tggtgacctc gttgttccac gaggggacac tgctctcgcg
     6661 ccagaccgag cgctacgacg cgtccctcac c
//
LOCUS       DASPGK010000370         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1494373, whole genome shotgun sequence.
ACCESSION   DASPGK010000370 DASPGK010000000
VERSION     DASPGK010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494373"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VEC56_10280"
     CDS             complement(<1..372)
                     /locus_tag="VEC56_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HXV14579.1"
                     /translation="MNEVSKRPTPREMERAFYGRDGSYDGVFFVGVRTTGVFCVPSCA
                     ARKPLARNVEYFASTADAEAAGYRPCRRCRPERAASAAPAWIDGLLRRVDRDPSARLS
                     DRDLTAMGLPPSRVRRYFRDRF"
     gene            complement(456..>2160)
                     /gene="recG"
                     /locus_tag="VEC56_10285"
     CDS             complement(456..>2160)
                     /gene="recG"
                     /locus_tag="VEC56_10285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXV14580.1"
                     /translation="NPEFELIGDEAGEPSGGDARTGRIVPVYRLTSGVSQRYLRSLVS
                     HALDTYRGVVTENLPARLVDERNLPSRADALQAMHFPDDEGLHARALERMKLEELFYM
                     QLILSVVRRRQSARSARPRLPVVFDLERRYLDALPFALTRAQRRALDEIHRDLTTERG
                     MNRLLQGDVGSGKTVVAGAALLAAVEAGYQAAFMAPTEILAIQHFRGLVPAFERLGVR
                     SSLLIGSLKPPDKKRVHEELRDGSIQVVVGTHALIQSEVAFRKLALVVIDEQHRFGVR
                     QRAALLKDDPHILVMTATPIPRTLALTAYADLDLSVIDEMPPGRAPIKTRIVPPEKRE
                     AMFAYIRDKQASGEQAYLLYPVIEETEKQDVEAAESAYAELSAGPLKGVPIAMLHGRM
                     SMGEKDEVMSAFHAGVVRVLIATTVVEVGVHVPQATIMAIHHAERFGLSQLHQLRGRV
                     GRGGREGYCFLLVGEGASPEGLERLDALVRESDGFKIAEEDLRLRGPGEFLGTRQHGV
                     PGFQVSNPLRDRELVERASAAVKSLLADDPNLDGSDGRRCRSHLRAVWSDDVAAASLS
                     M"
BASE COUNT          349 a          772 c          721 g          318 t
ORIGIN      
        1 gaagcggtcg cggaagtagc gccgcacacg cgacggaggc agtcccatcg cggtcagatc
       61 gcggtcggaa aggcgcgcgg aggggtcgcg atcgacgcgg cgcaggagcc catcgatcca
      121 cgcgggcgcg gccgacgccg cgcgctcggg acgacaacgc cggcacggcc tatagcccgc
      181 cgcctccgcg tccgcggtgg acgcgaagta ctcgacgttg cgcgcgagcg gcttgcgcgc
      241 cgcgcacgac ggcacgcaga acacgccggt ggtgcgcacg cccacgaaga acacgccgtc
      301 gtaggagccg tcgcgcccat agaaggcgcg ctccatctcc cgcggcgtcg gacgcttgga
      361 aacctcgttc atggatctac ctccgtgtct tcgcacggag catgggacgt cttggcattg
      421 cccgccaccg aggattgaac gtcgattctc ctaccctaca tcgacaagga ggccgccgcg
      481 acgtcgtcgg accagaccgc tcgcaggtgg gaccggcatc ggcgaccgtc ggaaccgtcc
      541 aggttcgggt cgtccgcgag caacgacttg accgccgcgc tggcgcgctc gaccagctcc
      601 cgatcgcgca gcggattcga cacctggaaa cccggaacgc cgtgctggcg cgtgcccagg
      661 aactcgcccg gcccgcgcag ccgcaggtct tcctccgcga tcttgaagcc gtccgactcg
      721 cgcaccaagg cgtcgagtcg ctccagtccc tccggcgaag ccccctcgcc caccaagagg
      781 aagcaatacc cctcgcgtcc cccgcgcccg acgcgcccgc gcagctggtg cagctgcgag
      841 agcccgaagc gttccgcgtg gtggatggcc atgatggtcg cctgcggcac gtgcacgccc
      901 acctcgacca ccgtggtcgc gatcaatacc cgtacgacgc cggcgtggaa cgccgacatc
      961 acctcgtcct tctcgcccat cgacatgcgg ccgtgcagca tcgcgatcgg aactcccttg
     1021 agcgggccgg ccgagagctc cgcgtacgcg ctctccgccg cttccacgtc ctgcttctcg
     1081 gtctcctcga tcacggggta caggaggtac gcctgctcgc ccgaggcctg cttgtcgcgg
     1141 atgtatgcga acatcgcctc gcgcttctcg ggcggaacga tgcgcgtctt gatgggcgcg
     1201 cggcccggcg gcatctcgtc gatcaccgag aggtcgaggt ccgcgtacgc ggtcagcgcg
     1261 agcgtgcgcg ggatcggggt ggcggtcatc accaggatgt gcgggtcgtc tttcaagagg
     1321 gccgcgcgct ggcgcacgcc gaagcggtgc tgctcgtcga tgacgaccaa ggccagcttg
     1381 cgaaacgcga cctcggattg gatgagcgcg tgcgtcccca ccaccacctg gatcgagccg
     1441 tcgcgaagct cctcgtgcac tcgtttcttg tccggcggct tgagcgaccc gatgagcaac
     1501 gacgagcgaa ctcccaagcg ctcgaaggcc ggcaccaggc cgcgaaagtg ctggatggcg
     1561 aggatctcgg tcggcgccat gaacgcggcc tgatagcccg cttcgaccgc cgcgaggaga
     1621 gcggctcccg cgaccacggt cttgcccgac ccgacgtctc cctgcaacag ccggttcatc
     1681 ccgcgctccg tcgtcagatc tcgatgaatc tcgtcgagcg cgcggcgctg ggcgcgcgtc
     1741 aaggcgaacg ggagcgcgtc gaggtagcgc cgctcgaggt cgaaaacgac cggcaggcgc
     1801 ggtcgcgccg agcgcgccga ctgccgccgt cgcaccaccg acaggatcaa ctgcatgtag
     1861 aacagctcct cgagcttcat gcgttcgagg gcgcgcgcgt gcaacccttc gtcgtcggga
     1921 aagtgcatgg cctggagcgc gtccgcgcgc gacgggagat tcctttcgtc gaccaggcgc
     1981 gcgggaagat tctccgtcac gacgccgcga tacgtatcga gcgcgtgcga gacgagcgag
     2041 cgcaggtagc gctggctcac cccgctcgtg agccggtaga ccggcacgat gcggccggtg
     2101 cgcgcgtcgc ctcccgacgg ctcgccggcc tcgtctccga tcaattcgaa ctcgggattg
//
LOCUS       DASPGK010000371         6474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_792741, whole genome shotgun sequence.
ACCESSION   DASPGK010000371 DASPGK010000000
VERSION     DASPGK010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792741"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1132)
                     /locus_tag="VEC56_10290"
     CDS             complement(92..1132)
                     /locus_tag="VEC56_10290"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HXV14581.1"
                     /translation="MKKEFDTPERVLDEIRGIYADPDPDLETRLVRELIVHALKCRRD
                     HLEVLDLKVLGRVMAEFRYAARVFKPYRDRRKVSIFGSARTPESDPYYIMAVDFARRL
                     AAEGFMVITGAAEGIMKAGNVGAGADASFGVNILLPFEQAPNPVIGDDPKLITFKYFF
                     VRKLFFLMEASAVALFPGGFGTHDEGFETMTLVQTGKAPPMPLLFMELPGEDYWESFD
                     RFIREQLLERKLISERDTKLYRICKSAQEGVDYVKFFYSTYHSMRFVRDRLVIRLEKE
                     LPDEAVARLASEFSDLVTKGTIQKTGALHEESNEPELDEKPRLIFTLNRQSPSRLYEM
                     ILAINELGRGVG"
     gene            complement(1134..1796)
                     /locus_tag="VEC56_10295"
     CDS             complement(1134..1796)
                     /locus_tag="VEC56_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXV14582.1"
                     /translation="MAHRPYDLICFDVDGTLVRHPSGKVIWEILNSRFTGDDSVNVQR
                     HRWYDEGKITYPQWVEMDVQGWIDARATRADIVDAIREFEHFEGAHDTLFELKRRGAR
                     LAVISGTLDIVIDTMFPEHPFDDIYSNRLLFDEHDRLTGWRATPFDLEGKPIALRELS
                     KKHDVALARAAFVGDGANDIPMVGVAGCVVAFNPRSRELERRANHVVREPNLSLLLEL
                     IG"
     gene            complement(1807..3768)
                     /locus_tag="VEC56_10300"
     CDS             complement(1807..3768)
                     /locus_tag="VEC56_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXV14583.1"
                     /translation="MSAPTRKLPPGALGGVSGDAYQPLVAPGERLPEFTWRSVGVGAL
                     LGIVFGAANAYLGLKVGITVSTSIPIAVMTFAILHVVRSRQGSMILEANMSQTVGSAS
                     SSLASGVIFTIPALFLWGLSPTLVELMLLSMCGGLLGALAMIPLRRPLIKKEHGMLPY
                     PEGTACAEILVASESGGSQARTVFSALGIGAVYKACTDIFHLWPEDFVQKIPKLPKGV
                     FTLEAAPMFLGVGYILGYNVAAVMVAGSLVSAMIFIPLIAHFGEYITMPLFPETTKPI
                     SQMSADEIWSRYIRYIGAGGVAAGGILTIIRTLPTMVRAFRTGLGEIVTRGETVATAL
                     RTDRDLSFRWVLLGAGSIVAVIALVPTILVHVDSFSMRLVAAVCMAGFAFFFVTVSSR
                     IVGYVGVSSNPTSGMTIATLIGTSSVFYLLGWTDTAGKVTALTVGTVVCTAASVAGDM
                     SQDLKTGFLLGATPKRQQAGELIGVLTSAAAVCGAVIVLNGQYGFGTKELPAPQATLM
                     KTVIEGILSANLPWTLVFIGALMALTVELVGIRALPFAVGLYLPLSSMSPIFVGGVLR
                     WWVERRNRNDEKRLHEARENGVLFGSGLVAGQGIMGVAIAGLAFGLGRRPDWGGHEWM
                     GAAGPYVALALFLTLGFYMVRVAQRTATR"
     gene            3847..4404
                     /locus_tag="VEC56_10305"
     CDS             3847..4404
                     /locus_tag="VEC56_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV14584.1"
                     /translation="MAVDREAPSRDPSRWVDEHGDALFRFAVLRVRDPDLAADLVQDT
                     FLSALKGIEGFRGGSSVRTWLTGILKHKIIDHYRRNKSEVLESDLHPEGDELAHAESL
                     RSAWKESPASLVEDREFWTVLTGCLDGLPEAHRRAFSMREFDGLSGEEIRKVLEVTPT
                     NLWVMLHRARTKLRRCLETNWFGEK"
     gene            4412..4666
                     /locus_tag="VEC56_10310"
     CDS             4412..4666
                     /locus_tag="VEC56_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV14585.1"
                     /translation="MNCKDAARLLSEKRDRRLSWRARISLRLHMLACKMCKVYGSQLD
                     TVGRVCREAGLRAEHNCPGELPDERKQRIKDAMTRDPRDR"
     gene            complement(4663..>6474)
                     /gene="asnB"
                     /locus_tag="VEC56_10315"
     CDS             complement(4663..>6474)
                     /gene="asnB"
                     /locus_tag="VEC56_10315"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV14586.1"
                     /translation="QALRAMCAAIRHRGPDDDGYFVQGGVGLGMRRLSIIDVAGGHQP
                     IFNEDRSCVIVYNGEIYNHRDVRRELEKRGHRYGTHSDTETILHAYEEYGVDCVKHLR
                     GMFAIAIWDEPRRRLFLARDRFGKKPLYYAHVGGRVVFGSELKAIVALPEVARRVNSQ
                     AVADYIAWGYVPDPLTIYQGIAKLPPAHWLTYEAGRVRVERYWDVSYAPVSARQPEEF
                     YVGRTLEILDEAVRIRLMSEVPLGAFLSGGTDSSIVVGLMARHSSERVKTFSIGFEES
                     AYNELPYARKVAGHFGTEHHEEIVRPDAERDVLSLVRQFDEPFADSSMLPTYYVSRAA
                     RKFVTVALSGDGGDELFAGYLRYVDDAWVRAASRLPAGVRDAVLGPLARALPAGTKGI
                     DRLRDLLGTPDDFYVRQMTRGIAHSARDVFADEFVRQLEHRDPAHVATPFLHAARGWD
                     ALSRRQYLDTNTYLPGDILTKVDRASMIVSLECRAPILDHELAEFAATIPVDLRIKGL
                     TTKYLLKKVAERLMPAELVHRPKMGFAIPVAYWLKGEWAARSEDLLLGKASLDRGVFR
                     EAFLRRVLSEHRSGKRDNSSMIWSLMMLELWWRNTDA"
BASE COUNT         1188 a         2127 c         2092 g         1067 t
ORIGIN      
        1 gccgattcct tcgacgcgtc cctactgcac gcgcacgaca gaatcgtcac tatcagcgcg
       61 gcccagaccg gtaccgtgag acgcatcgaa actaaccaac cccgcgaccc agctcgttga
      121 tcgcaaggat catctcatac aggcgcgacg gactctggcg gttgagcgtg aatatcaggc
      181 gcggcttctc gtcgagctcg ggttcattcg actcttcgtg gagggcgccc gtcttctgga
      241 tggtgccctt ggtcaccagg tcggagaact cgctcgcgag cctcgccacc gcttcgtccg
      301 ggagttcttt ctccagccgg atcacgagac gatcgcgcac gaagcgcatc gaatggtagg
      361 tcgagtagaa gaacttgacg tagtcgacgc cttcctgcgc cgacttgcaa atcctgtaca
      421 gcttggtgtc ccgctcggat atcaacttgc gttccagcag ctgctcgcga atgaaccgat
      481 cgaagctctc ccagtagtcc tcaccgggca gctccatgaa caacagtggc atcggcgggg
      541 cctttccggt ctgcacgagc gtcatggtct cgaagccctc gtcgtgggtg ccgaaaccgc
      601 ccgggaacag cgccaccgcg ctcgcttcca tcaagaagaa gagcttgcgc acgaagaaat
      661 atttgaatgt gatcagtttg gggtcgtcgc cgatgacggg attcggcgcc tgttcgaacg
      721 gcaagagaat gttgacgccg aagctcgcgt ccgcgcccgc gcccacgttg cccgctttca
      781 tgatgccctc ggccgcaccg gtgatgacca tgaaaccctc ggccgccagg cgccgcgcga
      841 agtcgaccgc catgatgtaa tacgggtcgc tctcgggcgt gcgcgccgat ccgaagatcg
      901 ataccttgcg ccggtcgcga tagggcttga acacgcgcgc ggcgtagcgg aactcggcca
      961 tgacgcgacc cagcaccttg agatcgagga cctcgaggtg gtcgcgacgg catttgagcg
     1021 cgtgcacgat caactcgcgc acgagccgcg tttccaagtc ggggtcggga tcggcgtaga
     1081 tgccgcgtat ctcgtccagc acgcgctcgg gcgtgtcgaa ttctttcttc atgtcatccg
     1141 atcagttcga ggagtaacga caggttgggt tcgcgcacga cgtgattggc gcggcgctcg
     1201 agctcgcggc tgcgcgggtt gaacgcgacc acgcagcccg cgacaccgac catggggatg
     1261 tcgttggcgc cgtcgccgac gaaggcggcg cgggcgagcg cgacgtcgtg cttcttcgac
     1321 agctcacgca acgcgatggg cttgccttcc aggtcgaagg gcgtggcgcg ccagcccgtc
     1381 aagcggtcgt gctcgtcgaa caagagccgg ttggagtaga tgtcgtcgaa cgggtgttcg
     1441 gggaacatgg tgtcgatcac gatgtcgagc gtacccgaga tcacggccag gcgcgcgccg
     1501 cgccgtttca gctcgaagag ggtgtcgtgc gcgccctcga agtgttcgaa ctcgcgaatc
     1561 gcgtccacga tgtcggcgcg cgtggcccgc gcgtcgatcc atccctgcac gtccatctcg
     1621 acccactggg ggtacgtgat cttgccttcg tcgtaccagc ggtggcgttg gacattgacg
     1681 gaatcgtcgc cggtgaatcg gctattcaga atctcccaga tgacctttcc cgatggatga
     1741 cgaacgaggg ttccgtcgac gtcgaagcag atgaggtcgt aggggcgatg cgccatgctc
     1801 gccattctac cgcgtcgccg tgcgttgcgc cacgcgcacc atataaaatc cgagggtcag
     1861 gaaaagcgcc agcgcgacgt agggccccgc cgctcccatc cactcgtgac cgccccaatc
     1921 cgggcgccgg cccagcccga acgcgaggcc ggcgatggcg acgcccatga tgccctgccc
     1981 cgccaccaag cccgacccaa acagcacccc gttctcgcgc gcctcgtgca ggcgtttttc
     2041 gtcgttccgg ttccggcgct ccacccacca acgcagcacg ccgcccacga aaatggggct
     2101 catcgacgat agtggaaggt agagcccaac cgcgaacggc agcgcgcgaa tgccgaccag
     2161 ctccaccgtc agcgccatca gcgcgccgat gaagaccagc gtccagggaa ggttggcgga
     2221 aaggattcct tcgatgacgg tcttcatgag cgtcgcttgg ggcgccggga gctccttggt
     2281 gccgaagccg tactgtccgt tgagcacgat caccgccccg cacaccgcgg ccgccgaggt
     2341 caagacgccg atcagctcgc ccgcctgctg gcgcttgggt gtggcaccca gcaagaaacc
     2401 cgtcttcaag tcctgcgaca tgtcgccggc gacgctggcc gccgtgcaca ccaccgttcc
     2461 caccgtgagg gccgtcactt tgccggcggt atcggtccag cccagcaaat agaacaccga
     2521 gctggtgccg atcagggtcg cgatggtcat gcccgaggtc gggttcgacg acacccccac
     2581 gtagccgacg atgcgcgacg acaccgtcac gaagaagaac gcgaagccgg ccatgcacac
     2641 cgcggcgacc aggcgcatcg aaaacgaatc gacgtggacc aagatggtcg gcacgagcgc
     2701 gatcaccgcc acgatcgagc ccgccccgag cagcacccag cgaaacgaga ggtcccggtc
     2761 ggtgcgaagc gcggtcgcca ccgtctcgcc ccgggtgacg atttcgccca agccggtgcg
     2821 gaacgcgcgc accatggtcg gcaaggtgcg aatgatggtg aggatgccgc ccgccgcgac
     2881 accgcccgcg ccgatgtagc ggatgtagcg cgaccagatc tcgtcggcgc tcatctgact
     2941 gatgggcttg gtcgtctcgg gaaagagcgg catcgtgatg tactcgccga agtgggcgat
     3001 gagcgggatg aagatcatcg cgctgaccaa gctgcccgcc accatcaccg ccgcgacgtt
     3061 gtaacccagg atgtagccga ctccgagaaa catcggcgcc gcttccagcg tgaacacgcc
     3121 cttgggcagc ttggggatct tctgcacgaa atcctcgggc cacagatgga agatgtcggt
     3181 gcaggccttg tagacggcac cgatcccgag cgccgaaaag accgtgcgcg cctgcgagcc
     3241 gccgctctcc gatgccacga ggatttccgc gcacgcggtt ccctccggat acgggagcat
     3301 tccgtgctcc ttcttgatga gcggccggcg caatggaatc atcgcgagag cacccaagag
     3361 cccgccgcac atcgacaaca acatcaactc gactagcgtc ggcgaaaggc cccagagaaa
     3421 cagcgcgggg atggtgaaga tgacgcccga ggcgagagac gaactcgccg agcccacggt
     3481 ctgcgacatg ttcgcttcca ggatcatcga gccctggcgc gagcgcacca cgtgcaggat
     3541 cgcgaaggtc atgaccgcga tcgggatcga cgtcgagacg gtgatgccga ctttgaggcc
     3601 gaggtatgcg ttggcggcgc cgaagacgat gccgagcagc gcgccgacgc ccaccgatcg
     3661 ccacgtgaac tcggggagtc gctctcccgg cgcgaccagc ggctgatacg cgtcacccga
     3721 caccccgccg agggcgccgg gtggaagctt gcgagtaggc gcggacatgg accggtagaa
     3781 tacgccgccg gcatcgaccg agtccaccct tgaaatcgcg cgcgcgtgcc ctagaatgac
     3841 gtggtcatgg ccgtcgatcg agaagcaccc tcccgcgacc cctcccggtg ggtcgacgag
     3901 cacggcgatg cgttgtttcg cttcgcggtg ctgcgcgtgc gcgaccccga cctggccgcg
     3961 gacttggtgc aggatacgtt tctctccgcg ctcaagggaa tcgaggggtt tcggggtggg
     4021 tcgtcggtcc gaacctggct caccggaatc ttgaagcaca agatcatcga ccactatcgc
     4081 cgcaacaagt ccgaggtcct cgagagcgac ctccaccccg agggtgacga gctcgcgcac
     4141 gcggagagcc ttcgctccgc ttggaaggaa tcgcccgcca gcctggtgga ggaccgggag
     4201 ttctggaccg tgctgaccgg ctgcctggac gggctccccg aggcgcaccg gcgcgcgttt
     4261 tcgatgcgcg agttcgacgg gctctcgggc gaagaaattc gtaaggtgct cgaggtgacc
     4321 ccgacgaacc tgtgggtcat gctccaccgc gcgcgcacga agctacggcg ctgcttggaa
     4381 acgaactggt tcggggaaaa atgagatgtc gttgaactgt aaagacgccg cgcgcctgct
     4441 ctccgagaag cgcgaccgcc gcctgtcgtg gcgagcacgc atctcacttc gcctgcacat
     4501 gctagcgtgc aagatgtgca aggtctacgg ctcgcagctc gacacggtgg gacgcgtctg
     4561 ccgcgaagcg ggcctgcgtg ccgagcacaa ttgtccgggc gaactcccgg acgagcgcaa
     4621 gcagcgaatc aaagacgcta tgacgcggga tccgcgcgat cgctaggcgt ccgtgttccg
     4681 ccaccacagt tccagcatca tcagagacca tatcatcgac gagttgtcgc gtttgccgga
     4741 tcggtgttcg ctcaagacgc gtcgcaggaa tgcctcccgg aacacgccgc gatcgagcga
     4801 agccttcccc aagagcaagt cttcgctgcg tgcggcccat tcccccttga gccagtaggc
     4861 gaccgggatc gcgaaaccca tcttgggccg gtggaccagc tccgccggca tcagccgctc
     4921 ggccaccttc ttcaggagat acttcgtcgt cagtcccttg attcgcaggt cgaccggaat
     4981 cgtggccgcg aactcggcca gctcgtggtc gaggatgggc gctcggcact cgagcgagac
     5041 tatcatgctg gcgcggtcga ccttggtcag gatgtcgccg ggtaggtagg tgttcgtgtc
     5101 caagtattgc cgccgcgaca gcgcgtccca tccgcgcgcc gcgtgcagga acggcgtggc
     5161 cacgtgggcc gggtcgcggt gctcgagctg ccgcacaaac tcgtccgcga acacgtcgcg
     5221 cgcggagtgc gcgatgccgc gcgtcatctg gcgaacgtag aagtcgtcgg gagtccccag
     5281 caggtcgcgc agcctatcga ttcccttggt acccgcgggc aaggcgcgcg cgagcggccc
     5341 cagcaccgcg tcgcgcaccc cggccggcaa ccggctcgcc gcgcgcaccc aggcgtcgtc
     5401 gacgtagcgc aggtacccgg cgaagagctc gtcgcccccg tcgcccgaga gcgccacggt
     5461 cacgaacttg cgcgccgcgc gcgacacgta gtaggtcggc aacatcgacg agtccgcgaa
     5521 aggctcgtcg aactggcgca cgagcgaaag gacgtcgcgt tcggcgtcgg gccgcacgat
     5581 ctcctcgtgg tgctcggtgc cgaagtgccc ggccaccttg cgcgcgtacg ggagctcgtt
     5641 gtacgcggat tcctcgaagc cgatcgaaaa cgtcttcacg cgctcgctcg agtggcgcgc
     5701 catcaagccg acgacgatcg acgagtcggt tccgccgctc aagaaggcac cgagtggcac
     5761 ctcgctcatc aagcgaatgc gaaccgcctc gtcgaggatt tcgagcgtcc gcccgacgta
     5821 gaactcctcg ggctggcgcg ccgacaccgg tgcgtaggac acgtcccagt agcgttccac
     5881 gcgcacgcgg ccggcttcgt aggtgagcca gtgcgccggg ggcagtttgg caatgccttg
     5941 atagatcgta agcgggtcgg ggacgtagcc ccacgcgatg tagtccgcga ccgcttgcga
     6001 gttcacgcgg cgcgcgacct cgggtagcgc aacgatcgcc ttgagctccg agccgaatac
     6061 cacccggccg ccgacgtgtg catagtacag gggcttctta ccgaagcgat cacgcgccag
     6121 gaacaagcgg cggcgaggtt cgtcccagat cgcgatggcg aacattcctc gcaagtgctt
     6181 gacgcagtcg acgccgtact cttcgtaggc atggaggatg gtttcggtgt cgctgtgggt
     6241 cccgtaacgg tgaccgcgct tctccaactc gcggcggacg tcgcggtggt tgtaaatctc
     6301 gccgttgtag acgatcacgc acgagcggtc ttcgttgaag atgggttggt ggccgcccgc
     6361 gacgtcgatg atggagaggc gtcgcatccc caatccgacg ccgccctgca cgaagtaccc
     6421 gtcgtcgtcg gggccgcggt ggcggatagc cgcgcacatg gccctgaggg cctg
//
LOCUS       DASPGK010000372         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_792877, whole genome shotgun sequence.
ACCESSION   DASPGK010000372 DASPGK010000000
VERSION     DASPGK010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792877"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEC56_10320"
     CDS             complement(<1..194)
                     /locus_tag="VEC56_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14587.1"
                     /translation="MQIARPHIGMARASDERMLVTWGALAPSFAVAGLVTVAVALLAL
                     SVGTVRIAPSTTLAVLLDHLP"
     gene            complement(194..1177)
                     /locus_tag="VEC56_10325"
     CDS             complement(194..1177)
                     /locus_tag="VEC56_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXV14588.1"
                     /translation="MTVTRNLLALMFVALLAFAFVACGDDDDAVDAGNAGDASPTAAA
                     SSGEFPITLERSDGADLTLEAPVERIVSLSPGATEIIYAVGAEDALIAVDNNANFPAE
                     VAALSGRVDAYQPNVETIAGFEPDLVIVANNIDGIVEALDRLDIPVWYVDLDTDVKTI
                     DDVLTQIDVLGQITGHKDASDALLADLNERIDAVKDELSSAEPAPEAPSVYHELDGEL
                     YSIADDTFIGSLYALLGARNIAGDGGGIAYPQLTQEAIIDGNPDVIVLADEAFGVTID
                     SVQARPGWDAVAAVQNERIYAINPDIISRPGPRIVDALEQLARDLYPQVFE"
     regulatory      complement(1327..1398)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1485..1874)
                     /locus_tag="VEC56_10330"
     CDS             complement(1485..1874)
                     /locus_tag="VEC56_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXV14589.1"
                     /translation="MNGKKPSSVDEYLSTLPAAPRATLEKVRAMIKGAVPDAAEGMAY
                     GMPGYKYDGRPLIYFAAAKNHLGLYGIPTVIEAHKAELAQYDLSKGVIRFPIDKAPTA
                     KLMKALLRARIAEIEAQNAGRKRKPKK"
BASE COUNT          333 a          722 c          592 g          366 t
ORIGIN      
        1 ggcaggtgat ccagcaacac cgccagcgtc gtcgaaggcg cgatccgcac cgtcccgacg
       61 ctcagcgcca gcagcgccac cgccaccgtc accagccccg ccaccgcgaa cgacggcgcg
      121 agcgcgcccc acgtgacgag catccgctcg tccgaagcgc gcgccatgcc gatgtgcggt
      181 cgcgcgatct gcattactcg aagacctggg ggtacaggtc gcgcgccagt tgctcgagcg
      241 cgtcgacgat ccgcggccct ggccggctga tgatgtccgg gttgatcgcg tagatgcgtt
      301 cgttctgcac cgccgcgacc gcgtcccacc cgggccgcgc ctgcaccgag tcgatcgtga
      361 cgccgaacgc ctcgtccgcc agcacgatca cgtcagggtt cccgtcgatg atggcttcct
      421 gcgtgagttg cggatacgcg atccctccgc catcccccgc gatgttgcgc gcgcccagca
      481 gcgcgtacag gctgccgatg aacgtgtcgt ccgcgatcga atacagctca ccgtccagct
      541 cgtgatagac gctcggtgcc tcgggagcag gctccgccga cgacagttcg tccttcaccg
      601 catcgatccg ctcgttcaga tccgcgagca gcgcgtccga tgcgtccttg tgcccggtga
      661 tctgccccag cacgtcgatc tgcgtcagca cgtcgtcgat cgtcttcacg tccgtgtcga
      721 ggtcgacgta ccacacggga atgtcgagcc ggtccagcgc ttcgactatg ccgtcgatgt
      781 tgttcgctac gatcacgagg tccggctcga accccgcgat cgtctccacg ttcggctggt
      841 acgcgtccac gcgccccgaa agcgccgcga cctccgcagg aaagttcgcg ttgttatcga
      901 ccgctatcag cgcgtcctcc gcgccgaccg cgtagatgat ctccgtagcc cccggcgaca
      961 gcgacacgat ccgctccacc ggcgcttcaa gcgtcaggtc cgcgccgtcg ctacgctcca
     1021 aagtgattgg aaattcgccg gacgatgctg ccgcagtcgg gctcgcatcg ccggcattgc
     1081 cggcatcgac cgcatcgtca tcgtcgccgc acgcgacaaa cgcgaaggca agcagcgcaa
     1141 cgaacatcag cgccaggaga ttgcgggtga cagtcatgac tcctcctcgg gtgactgact
     1201 gtcgcgggcg cttcatgaca taagaaatgc ccctgccatt cgaacagggg ctgcaggttg
     1261 cttcgagaga agcggcgtcc tgcgcctttt cctcgaaggc ctcgcgacag cgtgaggggc
     1321 agtcgacctg gctcccccga tagcagacgc gcctgcgccg gggatacagt tgcgggacag
     1381 cgccggcttc tcaccggctt cgccacgcct cacgaacacg tattcagttg ttatcgcgag
     1441 catacgcccg ccgctcgcgg accgtcaagc aagcggagcg ggcgtcactt cttcggcttc
     1501 cgcttcctcc ccgcgttctg cgcctcgatc tccgcgatcc gcgccctgag caaggctttc
     1561 atcaacttcg ccgtcggcgc cttgtcgata gggaagcgga tcacgccctt cgacaggtcg
     1621 tactgcgcca gctccgcctt gtgcgcctcg atcaccgtcg gaatcccgta cagccccagg
     1681 tggttcttcg ccgccgcgaa gtagatcagc ggccgtccgt cgtacttgta gccgggcata
     1741 ccgtacgcca tgccctcggc cgcatccggc accgccccct tgatcatcgc ccgcaccttt
     1801 tcgagcgtcg ctcgcggtgc cgcggggagt gtcgagagat actcgtctac gctggatggt
     1861 ttcttgccgt tcatcgtccg tttcctgctg ctccgccggc cgtcctcagg cccactatag
     1921 atacgacgaa cggaccgatt gtgaattgac ctacaagtgc accattgtta cgatcccaac
     1981 gaacctgggc tgaaggcgcg aacgcggcga aca
//
LOCUS       DASPGK010000373         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1376972, whole genome shotgun sequence.
ACCESSION   DASPGK010000373 DASPGK010000000
VERSION     DASPGK010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376972"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..890)
                     /gene="lepB"
                     /locus_tag="VEC56_10335"
     CDS             complement(294..890)
                     /gene="lepB"
                     /locus_tag="VEC56_10335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXV14590.1"
                     /translation="MAKRKKSVIREYAEIIVLAVALALFVRTFFVQAFRIPSESMEDT
                     LLVGDFLFANKLLYGPKLPLIDVRLPAIRDPEPGDIIIFKYPGDPKMDYIKRCVAVEG
                     QTVELVDNKLYVDGVMQDEEFTKYVFGSRPDRRFGPFEVPEGHIFMMGDNRDNSADSR
                     AWGPLDKKLISGKAMFIYFSWNPSAHSVRFSRIGDVIR"
     gene            complement(893..2704)
                     /gene="lepA"
                     /locus_tag="VEC56_10340"
     CDS             complement(893..2704)
                     /gene="lepA"
                     /locus_tag="VEC56_10340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXV14591.1"
                     /translation="MTFPQERIRNFCIIAHIDHGKSTLADRLLERTGTLTNKQMREQV
                     LDTMDLERERGITIKSHPIRMVYRARDGHDYLFNLIDTPGHVDFTYEVSRSLAACEGA
                     ILVVDAVQGIQAQTVSNMYLAAGNGLEILPVINKIDMPAARVEAVEHEIEDLLGASPD
                     EIVKVSAREGINIEALLEAVVERIPAPAGDREGQLKGLIFDSFYDTYKGSVVYVRVME
                     GRVKKGDKVRMWSKGNTFEAMETGYFRIAMIPSDVLEAGEVGYVVTGIKDIHDIKVGD
                     TITLDANPCDEPLPGYVDVKPMVFSGLYPVDAEDYEELREALDKLRLNDSSFIYEPET
                     SVALGFGFRCGFLGLLHMEVIQERLEREYNLDLITTMPNVEYVVRTKNGEEVVVDNPA
                     LFPPEGKIDHVREPFVRVEIVTPAEYVGGIMTLNQERRGVYIEMTYVTRDRAVLRYDM
                     PLAEILIDYYDRLKSLSRGYASLDYDYIGHRESNVVRLDIAINGDPVDALSMIVHHDS
                     AFMIGRDLASKLKEVIPRQMYEVAIQAVIGSKVISRTNVKALRKNVTAKCYGGDITRK
                     RKLLEKQKEGKRRMKMVGKVEIPQEAFLAVLKVERTS"
     gene            complement(2730..3452)
                     /locus_tag="VEC56_10345"
     CDS             complement(2730..3452)
                     /locus_tag="VEC56_10345"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HXV14592.1"
                     /translation="MRIPRIVAVVISVLVAGAGTSTRVARAAQAPADSVAADSSATPV
                     TASPAPDSLRAIVVHDSLGIPGTLKGEAVPDPAEVKAMLRRMGSSEVEGRTQWERKKN
                     PRVAMLCSALLPGLGQTYNGRRLKVGVMVGFTSFYFGSAWNNYKRYEASIQRRDTFQP
                     GTTPYVNENERAEFWKAEARTYVWWSGAVWIIGLLDSWIDAHLYDVREYTPPARAETS
                     GLPTIGAPTSYVTIGFDLYFAK"
     gene            complement(3517..3611)
                     /locus_tag="VEC56_10350"
     tRNA            complement(3517..3611)
                     /locus_tag="VEC56_10350"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3575..3577),aa:Sec,seq:tca)
BASE COUNT          661 a         1243 c         1200 g          738 t
ORIGIN      
        1 gacaaaggcg cgctcgcgct cgcgcgcggc cggcacgtgg tagaacgagc agtacgaaca
       61 ccgccgcgtg cagaagggga cgtggatata cagggagagc ggtttcattt caagatgcgt
      121 agtgatctcg gggcttgcgg agcgcggcga gcgccgaggg ttcccgacgc gaacttccgg
      181 gcggccggtg cgatcgatcc ctcgacgcga tttctgcagc gcgaatgtga caccgtattg
      241 gtcctctcgc gctgcagctt gagcggagag ggacgatcgt accggccgtc aaactaccga
      301 atcacgtcgc cgattctgga gaagcgaacc gagtgcgcgc tgggattcca ggagaagtag
      361 atgaacatcg ccttgcccga aatgagcttc ttgtccaacg ggccccacgc gcggctgtcc
      421 gcgctgttgt cgcggttgtc gcccatcatg aagatgtggc cctcgggcac ctcgaagggg
      481 ccgaagcgcc ggtcggggcg cgacccgaac acgtacttcg tgaactcctc gtcctgcatg
      541 acgccgtcga cgtagagctt gttgtcgacc aactcgaccg tttgtccctc gaccgcaacg
      601 cagcgcttga tgtagtccat cttgggatcg ccggggtact tgaagatgat gatatcgccc
      661 ggctcggggt cgcggatggc gggcagccgc acgtcgatga gcgggagctt gggaccgtac
      721 aggagcttgt tggcgaagag gaaatcgccc accagcagcg tgtcctccat cgactcggag
      781 ggaatgcgga acgcctggac gaagaacgtc cgcacgaaca gggccagagc caccgccagc
      841 acgatgatct cggcgtactc ccggatgacc gatttcttgc gttttgccac cgtcagctcg
      901 tcctctcgac tttgagcacg gccaggaagg cctcctgcgg gatctcgacc ttgccgacca
      961 tcttcatacg ccgtttgccc tctttctgtt tctccaagag tttacgcttt cgggtgatat
     1021 ccccgccata gcacttcgcg gtaacgttct tccgcagggc cttgacgttg gtccgggaga
     1081 tcaccttgga gcctatcacg gcctggatcg cgacctcata catttgccgc ggaatcactt
     1141 ccttcaactt cgatgccaag tcgcggccaa tcatgaaggc gctatcgtgg tggacgatca
     1201 tcgagagcgc atccaccggg tcgccgttga tcgcgatgtc gagacgcacc acgttgctct
     1261 cgcggtgccc gatgtagtca tagtccaggc tcgcgtagcc ccgggagaga ctcttgagtc
     1321 tatcatagta gtcgatcaag atctcggcca gcggcatgtc gtagcgcagg acggcccggt
     1381 cccgggtcac gtacgtcatc tcgatataca cgccgcgccg ctcctggttc aaggtcatga
     1441 tgccgcccac gtactcggcc ggggtcacga tctcgacccg cacgaagggc tcgcgcacgt
     1501 ggtcgatctt tccctcgggc ggaaagaggg ccgggttgtc gaccacgacc tcctcgccgt
     1561 tcttggtccg cacgacgtac tccacgttgg gcatggtggt aatgaggtcc aggttgtact
     1621 cgcgctcgag gcgctcctgg atcacttcca tgtgcaagag cccgaggaac ccgcatcgga
     1681 acccgaagcc caacgccacc gacgtctccg gctcgtagat gaacgaggag tcgttgaggc
     1741 gcagcttgtc gagggcttcg cgcaattctt cgtagtcttc ggcgtcgacg ggatagagcc
     1801 cgctgaacac catcggcttg acgtcgacgt agccgggcag cggttcgtcg cacgggttcg
     1861 cgtcgagcgt gatggtgtcg cccactttga tatcgtggat gtccttgatg ccggtgacga
     1921 cgtagcccac ttcgcccgcc tccagcacgt ccgacgggat catcgcgatg cggaaatagc
     1981 ccgtctccat cgcctcgaag gtgttcccct tcgaccacat gcgcaccttg tcgcccttct
     2041 tgacgcggcc ttccattacg cgcacgtaca cgaccgagcc cttgtaggtg tcgtagaacg
     2101 agtcgaagat caggcccttc agctggccct cgcggtcacc cgcgggcgcg ggaatgcgct
     2161 cgacgaccgc ctcgagtagg gcctcgatgt tgatgccttc gcgcgcgctc accttgacga
     2221 tttcgtccgg agaggcgccc agcaggtcct cgatctcgtg ctccactgct tccacgcgcg
     2281 cggccggcat gtcgatcttg ttgatcacgg gcaggatctc gagcccgttg cccgcggcga
     2341 gatacatgtt cgagacggtc tgggcctgga tgccctgcac cgcgtcgacc accaggatgg
     2401 cgccctcgca cgcggccagg ctgcgcgaga cctcgtaggt gaagtcgacg tgtcccggcg
     2461 tgtcgatcag gttgaagaga tagtcgtggc cgtcgcgcgc ccggtacacc atgcggatgg
     2521 gatggctctt gatggtgatg ccgcgctcgc gctcgaggtc catcgtgtcg agcacttgct
     2581 cgcgcatctg cttgttggtg agcgttcccg tgcgctcgag caggcggtcc gccaacgtcg
     2641 acttgccgtg gtcgatgtgc gcgatgatgc agaagttgcg aatgcgttct tgcggaaagg
     2701 tcatgtggtt tggtttcgcc tgccgtccat tatttcgcga agtagagatc gaatccgatg
     2761 gtcacgtaac tggtcggggc gccgatcgtt ggcaggcccg aggtctccgc tcgcgcgggc
     2821 ggcgtgtact cgcgcacgtc gtagaggtgc gcgtcgatcc acgagtccaa gagcccgatg
     2881 atccataccg cgcccgacca ccacacgtac gtgcgcgcct ctgccttcca aaactccgcg
     2941 cgctcgttct cgttcacgta gggtgtcgta cccggctgga aggtatcgcg gcgctggatc
     3001 gacgcctcgt agcgcttgta gttgttccac gcgctcccga agtagaacga tgtgaagccc
     3061 accatcacgc cgaccttcag ccgccgtcca ttatacgtct gccccaggcc cgggagcagc
     3121 gcgctgcaca acatcgccac acgcggattc ttctttcgct cccactgcgt gcggccttcg
     3181 acctcgctcg accccatgcg ccggagcatc gccttcacct cggcggggtc cggcacggct
     3241 tcgcccttga gcgtccccgg aattccgagt gagtcgtgca cgacaatcgc gcgcagcgaa
     3301 tccggcgcgg gactcgccgt cacgggggtg gccgacgaat cggcggccac cgagtccgcg
     3361 ggtgcttgcg ccgcgcgcgc gacccgcgtc gaggtacccg ccccggcgac cagcaccgag
     3421 atcacgacgg cgacgatcct tggaatacgc aacggccttc gctcaaactt cggggacgtg
     3481 acatcgggcg cgacccgccg tcgcgccacg gacagatggc gggaatgtgt gggaatcgaa
     3541 cccacctcgg acggcatcgc ccgacatagg gatttgaagt ccccgaggtc caccagggcc
     3601 catccattcc cgaggcggtc gtcccggccc aataaacgcg acatgccctt gcgggcatgt
     3661 tcggcttgag aatataacag atgcccggag agaccgagaa gcgcgaactt ggcgtccccg
     3721 gggcggtata agtcgttgcg cccgcggccg caagaacgcg cttgcgcgtc ccgccgcggg
     3781 atgcttttct taagcgaaac gcgccggccg cggcccctcc cgcccagttc ggccggtcac
     3841 cc
//
LOCUS       DASPGK010000374         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_793753, whole genome shotgun sequence.
ACCESSION   DASPGK010000374 DASPGK010000000
VERSION     DASPGK010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793753"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VEC56_10355"
     CDS             <1..544
                     /locus_tag="VEC56_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV14593.1"
                     /translation="FKEGTETIMHQVDVLKRIASEFSSYGRMQQLHVKPQAVDPLVRG
                     IVQPYEQNTSGVRVIYENGASNAKVMADGEAVRKICANLIENALEAMGTKGGELRVRC
                     AETTNEGAPAIRISFRDTGPGLNEEVTRRLFEPYFSTKTTGTGLGLAICRTLSREMGG
                     DVTLENVAGGVEAALVLKKA"
     gene            complement(601..1638)
                     /locus_tag="VEC56_10360"
     CDS             complement(601..1638)
                     /locus_tag="VEC56_10360"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HXV14594.1"
                     /translation="MMRHSWVIFSLSLTLAACGGYDRELMGDPVPEPAQVAAAEVTYE
                     ETEEVPVEPADSLAMFDDLRFYGSWYELYPYGMVWRPVVVSTWAPMSYGHWVWTSYGW
                     MWVSYDPFGSLVYQYGFWVNDFALGWVWVPDYVWAPVQCEWVWWDDYIGWCPLPPPGV
                     RYKDPWEYTDSDPWITVRVTKFKETDVAHYKTTPKFKSGYSERTLRREAPDAGTIERG
                     LGRPVRVVDVQIDKIAVGQHELTRVVLPHDEQRIIEKERASRPGGAVRPSPEARFKQP
                     PPNSGGTGSDRTKDEVSPPPKEKATTTPPPKEKSTSSPPPKKESPPKFKEKDKEKDSS
                     SDKKDGEKKKG"
     gene            1862..2569
                     /locus_tag="VEC56_10365"
     CDS             1862..2569
                     /locus_tag="VEC56_10365"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HXV14595.1"
                     /translation="MSTRTGWGMAAIVMMAVAIPGPGYAQTARGNPAEEYGVRVARVK
                     YGGGGDWYNNPSSIPNWLSEFERRTGVHTVKQEKVLALTDENLRAYPFLYMTGHGTIK
                     LGSDERESLRAYLEDGGFLFINDSYGLDPSVRAMVRELFPERAFEELPNDHPIYHSFY
                     DLPGLPKIHEHDKKSPQGFGVTIDDRLVIYYVYESDIGDGLADPEVHHDTPEKRELAM
                     KMAVNVLIYAITQNVMM"
     gene            2580..>5371
                     /locus_tag="VEC56_10370"
     CDS             2580..>5371
                     /locus_tag="VEC56_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HXV14596.1"
                     /translation="MSGNDALRGYLRSILWRTAGLHAVSGMVSFLAALSWVLLAVVVW
                     TAIVDTPSLAASTFVARATIVIAALMFGYFVVWPLVRMPRLNQLAAEIERRQDLQEMV
                     RAGFEFSQDESATRRYSPDLVKEVIRQAVEKLSGLHVRSIFLNRRDMALVPVALAGLI
                     VLLGISLLKPSMVTEAGRRVFSPEDVAALPHRPNIYAQPGDVTVLAGSNVTVSGLDLG
                     RSDSQVQIAFNLSNDFWKSEPTTRTAGVAHADAAFDRYDYTFRDIRNTTSYRFQVGDY
                     QSPTYTITVVHEPILTDVRVTLTPPAYTGEPTTTLDQSAGNIQALEGTRVKVEARSNN
                     ALRAAWVQFDEKPKQAVAFLDRDLSFDFAALADGRYRVLLEDTLGFATRDPLTYTVEV
                     FQDRPPTVDVLEPGADMPMPRAQTVDIGFVAADDYGLARASLHWRKVGDEAFRTQGIG
                     LGDQRGKREIAVAHRWDLTSVTLFPGNSIEYFVEVADNNTVTGPGVARSDVFRLTMPT
                     IGEIYDTAREQENARAEAMESAIEESRRLSEQLEKLQREYTKTEKMDWAQKKELDRAA
                     ETQKAVEQKLGEVKESLDKTLRELSDNEMTSQQIGEKLEEIRQLMEEIDSEQLEKYMD
                     NLRKAMEKMSPEEIRQALENMQLDTKEMLERLERTASLLRELQKEQRMEELVRQTQDL
                     MEKQGELNEKTGEANPKDGEKMSDLAKQQEELAKQADDMQKSAQDLEQQLDDPSAKQE
                     LQQMSQEMKQDGPQQNMREASKDLQQQQQQQAMQQQQEAMEKMVSLFKRATKAQQMQS
                     QSQGQQMATNFQKFAKQTLELSHRQESLAGDLKESSGSETQSFQEISTDQLSYLEATE
                     KVANEINKIASLSLSVSPVLLQSLGEAITRMQSSMMFLEQDKPYMSTTHAHNAVESLN
                     QATIEMLRSAKSCQQG"
BASE COUNT         1081 a         1691 c         1753 g          846 t
ORIGIN      
        1 gttcaaagag ggcaccgaga ccatcatgca ccaggtggac gtcttgaagc gcatcgccag
       61 cgaattttcg tcctacgggc gcatgcagca gttgcacgtc aaaccgcaag cggtcgaccc
      121 gttggtgcgc gggatcgtgc agccctacga gcagaacacg tccggcgtgc gcgtgatcta
      181 cgagaacggc gcgtcgaacg cgaaggtgat ggccgacggt gaagcggtgc gcaagatttg
      241 cgcgaatctg atcgagaacg cgctggaagc gatggggacg aagggcggcg agttgcgcgt
      301 gcgctgcgcg gagaccacca acgaaggagc gcccgcgatt cggatcagct tccgcgacac
      361 ggggccgggt ttgaacgaag aagtcacacg gcgtctcttc gaaccgtatt tttccaccaa
      421 gacgacgggg acggggttgg ggctcgcgat ctgtcgcacg ctatcgcgcg agatgggcgg
      481 cgacgtgacg ttggaaaacg tcgcgggggg cgtcgaggcg gcgctggtgc tgaagaaggc
      541 gtgaactaga ccgacgatcg cgtgagcacc gccccggtct tcgcgctacc gaaaccagca
      601 ctaccccttc ttcttctcgc cgtccttctt atccgacgaa gaatctttct ccttatcctt
      661 ctccttgaac tttggaggcg actccttctt gggcggcggg gacgacgtgg acttctcctt
      721 cggcggggga gtcgtcgtcg ccttctcctt gggcggcggc gaaacctcgt ccttggtcct
      781 gtccgagccg gtaccccccg agttcggggg aggctgtttg aacctcgcct cgggagacgg
      841 tcgaacggcg ccgccgggcc gcgaagcgcg ttccttctcg atgatccgtt gctcgtcgtg
      901 cgggagaacg acgcgcgtga gctcgtgctg gccgacggca atcttgtcga tctgaacgtc
      961 gacgaccctg accggccggc cgagtccacg ctcgatggta cccgcgtcgg gtgcttcgcg
     1021 gcgaagggtg cgctccgaat aacccgactt gaacttcggc gtcgtcttgt agtgcgcgac
     1081 gtcggtctcc ttgaacttgg tcactcggac ggtgatccac gggtcggagt cggtgtactc
     1141 ccacggatcc ttgtagcgaa cgcccggcgg ggggagcgga caccatccga tgtagtcgtc
     1201 ccaccagacc cactcgcatt gtaccggcgc ccacacgtag tccgggaccc acacccagcc
     1261 gagggcgaaa tcgttgaccc agaagccgta ctggtacacg agcgacccga agggatcgta
     1321 cgagacccac atccagccgt atgaggtcca cacccagtgg ccgtacgaca tgggcgccca
     1381 ggtggaaacc accaccggcc gccacaccat cccgtaagga tagagctcgt accagctccc
     1441 gtagaacctg aggtcgtcga acatggcgag cgagtcggcg ggctcgaccg ggacctcttc
     1501 ggtctcttcg taggtgacct cggcggcagc gacctgggcg ggctccggta ccgggtcgcc
     1561 catgagctcg cggtcgtaac cgccgcaggc ggccagcgtc agcgagaggg aaaatatgac
     1621 ccaactgtgt ctcatcatgt ccgtatagtc cgaatcgggg agggtgattc cgaagattcc
     1681 caggcaccgg gcccggccct ccggcgtttg aatactaggc agcgagcgcg gggcacgcaa
     1741 cgtgcaagtc gttgcctgag aacgtcgccc ggaccgcctt gtgcggcggc cgggcctttg
     1801 ctttcgccca tcgaagtaac tataatggat gacagcgagt tatctcatgc ggatgggact
     1861 tttgagcaca cggacgggct ggggcatggc ggcgatcgta atgatggccg tcgcgatccc
     1921 cggcccgggc tacgcccaga cggcacgggg taacccggcc gaggagtacg gggtccgggt
     1981 ggcgcgggtg aagtatggcg gcggcggcga ctggtacaac aacccgtcct cgattcccaa
     2041 ttggctctcc gagttcgagc gccgcaccgg ggttcacacc gtcaaacagg agaaagtgct
     2101 cgccctcacc gacgagaatc tgcgcgcgta cccgttcctc tacatgaccg gacacggcac
     2161 catcaagctt ggcagcgacg agcgcgaatc gctgcgcgcg tatctcgagg acggcggctt
     2221 cttgtttatc aatgactcct acggcctcga cccgtcggtg cgcgcgatgg tgcgcgagtt
     2281 gttcccggag cgcgcgttcg aggaactccc caacgaccat cccatatatc acagcttcta
     2341 cgatctgccc gggcttccga agatccacga gcacgacaag aagtcgccgc agggattcgg
     2401 cgtcaccatc gacgaccgcc tggtgatcta ttacgtctac gaatcggata tcggcgacgg
     2461 cctcgcggac ccggaggtcc accacgacac gcccgagaag cgcgagctcg cgatgaagat
     2521 ggcagtgaac gtcctcatat acgccatcac gcagaacgtg atgatgtagg aaggttacga
     2581 tgagcggcaa cgacgcactg cgcggttacc tccgatcgat cctgtggcgc acagccgggc
     2641 tccacgccgt ttccggcatg gtgtcgttcc tcgccgccct ctcgtgggtg ctgctcgcgg
     2701 tggtggtgtg gacggccatc gtcgacacgc cgagtctggc tgcgtcgacc ttcgtcgcgc
     2761 gcgcgacgat cgtgatcgca gcgctcatgt tcggttactt cgtggtatgg ccgctcgtgc
     2821 gcatgccgcg cctgaatcag ctggccgccg agatcgagcg ccgccaggac ctgcaagaga
     2881 tggtgcgcgc gggcttcgag ttcagccagg acgaaagcgc gacgcggcgc tactcgcccg
     2941 acctcgtcaa ggaagtcatc cggcaagcgg tcgagaagct ctcggggctg cacgtgcgtt
     3001 cgatcttcct caatcgacgc gacatggcgc tggtgccggt cgcgctcgcg gggttgatcg
     3061 tgctgctcgg catctcgctc ttgaagccgt ctatggtcac cgaagcgggc cggcgcgtct
     3121 tctcgcccga ggacgtcgcg gcgctcccgc accgtcccaa catctacgcc caaccgggcg
     3181 acgtcaccgt gctcgcaggc tcgaacgtca ccgtgagcgg tttggacctc ggtcgcagcg
     3241 acagccaagt ccagatcgcc ttcaatctct ccaacgattt ctggaagagc gagccgacca
     3301 cgcgtaccgc gggtgtcgcg cacgcggacg cggccttcga tcgctacgat tacaccttcc
     3361 gtgacatccg caacacgacg tcgtatcgct tccaggtggg cgactaccag agcccaacct
     3421 acaccatcac ggtcgtacac gagcccatcc tcaccgacgt gcgcgtgacg ctcacaccgc
     3481 ccgcgtacac gggcgagccg accaccacgc tggaccagag cgcgggcaac atccaggcgc
     3541 tggaagggac gcgcgtcaag gtcgaagcgc gcagcaacaa tgcgctgcga gcggcctggg
     3601 tccagttcga cgagaagcct aaacaagcgg tcgcgttctt ggaccgcgat ctatcgttcg
     3661 acttcgcggc cctcgcggac gggcgctacc gcgtgctgct cgaagacact ctgggcttcg
     3721 cgacgcgcga cccgctgacg tacacggtcg aggtgttcca ggatcgcccg cccacggtgg
     3781 acgtactcga gccgggcgcg gacatgccga tgccgcgcgc gcagaccgtc gacatcggct
     3841 tcgtggccgc cgacgactac gggctcgctc gcgcctcgct gcactggcgc aaggtcggcg
     3901 acgaggcgtt ccgcacgcag ggcatcggcc tgggcgacca gcgcggcaag cgcgagatcg
     3961 cggtggcgca ccgctgggac ttgacgtcgg tgacgctgtt cccgggcaac tccatcgagt
     4021 acttcgtcga agtggccgac aacaacaccg tcacgggtcc cggggtcgcg cgcagcgacg
     4081 tgttccgtct caccatgccg accatcggcg agatctacga caccgcgcgt gagcaggaga
     4141 acgcgcgcgc ggaggcgatg gaaagcgcca tcgaagagag ccgccgcttg agcgagcagc
     4201 tcgagaagct ccagcgcgag tacaccaaga ccgagaagat ggactgggcg cagaagaagg
     4261 agctggaccg cgccgccgag acgcagaagg cggtcgagca gaagctcggc gaagtgaagg
     4321 agtcgctcga caagacgctg cgcgagctgt cggacaacga gatgacctcg cagcagatcg
     4381 gcgagaagct ggaagagatt cgccagctca tggaggagat cgactccgag cagctcgaga
     4441 agtacatgga caacctgcgc aaggccatgg agaagatgag cccggaggag attcgccagg
     4501 cgctcgagaa catgcagctc gacaccaagg agatgctgga gcggctcgaa cggaccgcga
     4561 gcctgttgcg cgagcttcag aaggagcagc gcatggaaga gctcgtgcgg cagacgcagg
     4621 acctcatgga gaagcagggt gagctcaacg agaagacggg cgaagccaac ccgaaagacg
     4681 gcgagaagat gagcgacctc gccaagcagc aggaagagct cgcgaagcaa gcggacgaca
     4741 tgcaaaagag cgcccaggac ctcgagcagc agttggacga cccgtcggcc aagcaagagc
     4801 tgcagcagat gtcgcaagaa atgaaacagg acggcccgca gcagaacatg cgcgaagcct
     4861 cgaaggacct gcagcagcag caacagcagc aagccatgca gcagcagcag gaagccatgg
     4921 agaagatggt cagcctgttc aagcgcgcca ccaaggcgca gcagatgcag tcgcagagcc
     4981 agggccagca gatggcgacg aacttccaga agttcgccaa gcagaccctg gagctgtcgc
     5041 accgtcagga gtcgctggcg ggcgacctca aggagtcctc ggggtcggag acacaaagct
     5101 tccaggagat ctcgaccgac cagctgagct acctcgaggc gaccgagaag gtcgccaacg
     5161 agatcaacaa gatcgcgagc ttgtcgctct cggtgtcgcc ggtgttgctg cagtcgctgg
     5221 gcgaagcgat cacgcgcatg cagagctcga tgatgtttct cgagcaggac aagccctaca
     5281 tgtcgaccac gcacgcgcac aacgcggtcg agtcgctgaa ccaggccacc atcgagatgc
     5341 tgcgcagcgc caagagctgc cagcagggac a
//
LOCUS       DASPGK010000375         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1377615, whole genome shotgun sequence.
ACCESSION   DASPGK010000375 DASPGK010000000
VERSION     DASPGK010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377615"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VEC56_10375"
     CDS             complement(<1..1185)
                     /locus_tag="VEC56_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HXV14597.1"
                     /translation="MTSRVEQIALAGKPLAYAPHEKLGVVVVDSFPALGTLAALRFLE
                     WVQSNPEGVVSLPTGKTPEFFIKEVGRFTSGWKKKAIQAELAERGIDPSVKCSLKGLH
                     FVQIDEFYPIDPSHHNSFLYYVNHYYIEGFGLDRARALLIDCSRIGLPPGKTLSDVWP
                     DDDVDLSLRYRMAGSKHEQLQRDVVQQIDEWCTEYEEKIRALGGIGFFLGGIGPDGHI
                     GFNVRGSDPFSTTRLTAVNYETQAAAATDLGGIEVARKRLVITIGLSTITHNPECTAV
                     VIAAGEAKAGIVRDAIQNERHVRYPSTALQVLPNARFYLTQGAAKKLEARQLAALVAS
                     ERATAAQIEQAVIDLAMAHRKPIAALTEADFRKNPHAAELLKQGIGSAKDVCARTVQR
                     LKE"
     gene            1362..2966
                     /locus_tag="VEC56_10380"
     CDS             1362..2966
                     /locus_tag="VEC56_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HXV14598.1"
                     /translation="MTRSRTPLWILFAALAVTIACGQDDPVALDSTDPVDPAEVNQFV
                     NGLPNWEIPSDFEEPPVDLGAEEDFEASQYYRCEAVEYDLKRNFEDIIAVGANATALK
                     PGMIVQGNGVKDGSLSTIGLKRSPLSLSVNLALADPSRTVDAPTSSTIQDAIASLQRD
                     ADDRLGNLDVIPAQINFRAESAYSVQQALITAGLSVRYSGIIASGSVGGSLSQSTSVK
                     KRSVVVKLMQPMYTISFADDEKAEPADFFASDLMEADFERQRQQGTMGPGNLPCYVQS
                     VTYGRMVVYSMTTTETTSDEEYKLAVQASYGAWSGSGSINTRQRELVQNSTVEVQVFG
                     GTQGAGLDAIKQALRNGDFSAFLQPAPATTAVPLSYRINDLKNRQPAVIGDATKFTIR
                     ECEPVNDLQFTVTLDSIIVVDGCVPETSFDIESWVYPPQQGRSYWLLHNTGTRFPKET
                     LPSLHSQAIFTMTVNQASTIEFFTGVYGNGTTGLDGWSKETVFAFPFDFDENPYFFTH
                     LETTSDNLYRNCTLQVFYSIKRELAN"
     gene            complement(3037..>3299)
                     /locus_tag="VEC56_10385"
     CDS             complement(3037..>3299)
                     /locus_tag="VEC56_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14599.1"
                     /translation="ESLVGEGLKRLRAMESGGPPRPRDVRALIGLGRCRLAQGDVVGA
                     ETYFRQALEIERRFRKPGHPRIALAEAALAEATEAGAAASSR"
BASE COUNT          567 a         1109 c         1063 g          560 t
ORIGIN      
        1 ttccttcaag cgctgcaccg tgcgcgcaca cacgtccttc gcgcttccga tgccctgctt
       61 caagagctcc gcggcgtggg ggttcttgcg gaagtccgcc tcggtcagcg cagcgatggg
      121 cttgcggtgc gccatcgcca agtcgatgac cgcctgctcg atctgcgcgg cggtcgcgcg
      181 ctcgctcgcg acgagcgcgg cgagctggcg cgcctcgagc ttcttggccg cgccctgggt
      241 gagatagaag cgcgcattgg gcagcacctg caacgcggtg gacggatatc gcacgtggcg
      301 ctcgttctgg atcgcgtcgc gcacgatgcc cgccttggct tccccggccg cgatcacgac
      361 ggcggtgcat tcggggttgt gcgtgatggt cgagaggccg atggtaatca cgaggcgctt
      421 gcgggcgact tcgataccgc ccaagtcggt ggccgccgcc gcctgcgtct cgtagttgac
      481 cgcggtcaag cgcgtggtcg aaaacggatc cgacccgcgc acgttgaagc cgatgtgtcc
      541 gtcgggaccg ataccgccca agaaaaatcc aatgcccccg agcgcgcgga tcttttcttc
      601 gtactcggtg caccactcgt cgatctgttg gacgacgtcg cgctggagct gctcgtgctt
      661 cgaccccgcc atgcggtagc gcaggctcaa gtcgacgtcg tcgtcgggcc acacgtcgga
      721 cagcgtcttc cccggtggca agccgatgcg gctgcaatcg atcagcagcg cgcgcgcgcg
      781 atcgagcccg aagccttcga tgtaatagtg attgacgtag tagaggaagc tgttgtggtg
      841 cgagggatcg atgggataga actcgtcgat ctgcacgaag tgcagtccct tcaacgagca
      901 cttcacgctc gggtcgatgc cccgctcggc cagctcggct tggatcgcct tcttcttcca
      961 cccgctcgtg aagcgcccga cttccttgat gaagaattcc ggcgtcttcc ccgtgggcaa
     1021 cgacaccacg ccttcgggat tcgattgcac ccactccaag aagcggagcg cggccagcgt
     1081 ccctagggcc ggaaacgaat cgaccacgac cacgcccagc ttttcgtgcg gggcgtatgc
     1141 gagcggcttt cccgcgagcg cgatctgttc gacgcgtgag gtcatcgggt tgggcggcgc
     1201 tgccaggcgc ggcggcaaaa ctatagcccg cggaagcgat tggcgataag cacgttttgg
     1261 cgtgatcgct tttctcccgg tttcctgcgt tcgggggtga ggccgaacgg cggagcaccg
     1321 ccccggcccc cacaagccca aaccaggacc ggaggtccac catgactcgc tcccgaacgc
     1381 ccctatggat tttattcgcc gcgctggcgg tcacgatcgc ttgcggccag gacgaccccg
     1441 tcgcgctcga ctcgaccgat cccgtcgacc cggccgaggt caatcagttc gtcaacggcc
     1501 tccccaactg ggagatcccg tccgacttcg aagagccgcc cgtcgacctg ggagcggagg
     1561 aagacttcga ggccagccag tactaccgct gcgaagcggt cgagtacgac ttgaagcgca
     1621 acttcgagga catcatcgcg gtgggcgcca acgcgaccgc gctcaagccg ggcatgatcg
     1681 tgcagggcaa cggcgtcaag gacggctcgc tctcgaccat cgggttgaag cgctcgccgc
     1741 tctcgctttc ggtcaacttg gcgctcgccg acccgtcgcg caccgtcgac gcgcccacca
     1801 gctcgaccat ccaggacgcg atcgcgtcgc tccagcgcga cgcggacgac cgcctgggca
     1861 atctcgacgt catccccgcc cagatcaact tccgcgcgga gtcggcgtac tcggtgcagc
     1921 aggcgctgat caccgcgggg ttgagcgtgc gctacagcgg catcattgcc agcggatcgg
     1981 tgggtgggtc gctctcgcag tcaacgtcgg tgaagaagcg cagcgtggtc gtcaagctga
     2041 tgcagcccat gtacacgatc tctttcgccg acgacgagaa ggcggagccc gcggacttct
     2101 tcgcttccga tttgatggag gccgacttcg agcgccagcg ccagcaaggc acgatgggcc
     2161 ccggcaacct cccctgctac gtgcagtcgg tgacctacgg gcgcatggtg gtgtactcga
     2221 tgaccaccac cgagaccacc agcgacgagg agtacaagct cgcggtgcag gcgtcctacg
     2281 gcgcgtggtc ggggtccggg agcatcaaca cgcgccagcg cgagctggtg cagaactcca
     2341 ccgtcgaagt gcaggtgttc ggcgggacgc agggtgcggg actggatgcg atcaagcagg
     2401 cgctacggaa cggcgacttc agcgccttcc tgcaacccgc acccgccacc accgcggtgc
     2461 cgctttcgta tcgcatcaac gacctgaaga accgccagcc ggccgtcatc ggcgacgcca
     2521 ccaagttcac gatccgcgag tgcgaacccg tcaacgacct gcagttcacc gtcacgctcg
     2581 acagcatcat cgtggtcgac ggctgcgtgc cggagaccag cttcgacatc gagtcttggg
     2641 tgtacccgcc ccagcaggga cggtcgtact ggctcttgca caacacgggg acgcgatttc
     2701 ccaaggagac gctgccgtcg ctccacagcc aggccatctt caccatgacg gtcaatcagg
     2761 cgtcgaccat tgagttcttc accggcgtgt acggcaacgg gaccacgggg ctcgacggat
     2821 ggagcaagga gaccgtcttc gcgtttccgt tcgacttcga cgagaacccc tacttcttca
     2881 cgcacctcga gacgacgtcg gacaacctgt accgcaactg cacgctgcag gtgttctact
     2941 cgatcaagcg agagctcgcg aattgacaag gagtccgcgt gcgcgcgggc ttgtggaggg
     3001 agcgccggtc atgacctcgg gccggcgctt ccgcgttcag cgagaggacg cggctgcgcc
     3061 ggcctcggtc gcttcggcca gcgcggcttc ggcgagcgcg atcctcgggt ggccgggctt
     3121 gcgaaagcgg cgctcgatct cgagcgcctg acggaagtac gtctccgcgc cgacgacgtc
     3181 cccttgcgcc aaacggcagc gaccgagccc gatgagcgcg cgaacgtcgc gcgggcgggg
     3241 ggggccgccg gactccatcg cccgcaggcg tttgaggccc tcgccgacca acgactccg
//
LOCUS       DASPGK010000376         7287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_92060, whole genome shotgun sequence.
ACCESSION   DASPGK010000376 DASPGK010000000
VERSION     DASPGK010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92060"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEC56_10390"
     CDS             <1..244
                     /locus_tag="VEC56_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXV14600.1"
                     /translation="FYLAPEGIVYPCLTIPSPMGDLKGRSFEDLWESPEADTVRRQID
                     GCEQCWMICTARSSLKKNLPRALGWIAREKFRAHAH"
     gene            339..1505
                     /locus_tag="VEC56_10395"
     CDS             339..1505
                     /locus_tag="VEC56_10395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV14601.1"
                     /translation="MTRRTGMRPLRIAMVGQKGIPARYGGVETHVENVAVRLAARGHD
                     VTVFCRRRLRANGDAQETPSRNGTYRGVHLLYRASVNTKHFDAATHAFLSTLESSCRH
                     HFDVVHLHGIGPAAFAPVAGLFGRKVVSTFHALDWRQVKWGPRAKSLLRRGEAIGARA
                     SDGVIAVSRLMQVHIQSTHGVDAAYIPNGATLPAKPVSAGSLSQWGLVPGEYLLTVGR
                     VIADRDLETLLRAFASIRDRHPRLRLVIVGSETPRTVYSELLERMAGERVVFTGDVFG
                     EALQALYAHCLVYVLASRVEGLPITVCEAMAHGRALVLSDIPENAEVGGDTARFFKCG
                     DDSALANQLHPLIEDRAARDALGAVARARCEAHYNWEHIADQVESLYYRVLSHA"
     gene            1569..2114
                     /locus_tag="VEC56_10400"
     CDS             1569..2114
                     /locus_tag="VEC56_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV14602.1"
                     /translation="MKRALLTTMLVATAAAWLSGCATVPSSSGTPSRPASVERASDRN
                     PYDFRSEGKIPPLSPSDAPNEPDVEEMGITETAIESSDAEAPPDTAARAPEPTDTLVD
                     GFRVQVFASADREIAENAARGAQERLGLASYVELDGGVYKVRVGDYAARAQADQALAA
                     VRRHYADAWIVPSKVRAAQRP"
     gene            2185..2259
                     /locus_tag="VEC56_10405"
     tRNA            2185..2259
                     /locus_tag="VEC56_10405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2217..2219,aa:Gly,seq:gcc)
     gene            2325..2399
                     /locus_tag="VEC56_10410"
     tRNA            2325..2399
                     /locus_tag="VEC56_10410"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2357..2359,aa:Cys,seq:gca)
     gene            complement(2495..3400)
                     /locus_tag="VEC56_10415"
     CDS             complement(2495..3400)
                     /locus_tag="VEC56_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14603.1"
                     /translation="MSKRYRMGFVVAAMAAVLALPALAAVDEPSIGVGASGHAKQAIT
                     ITAGPSGLPNGFAIWWMDRSTFLSNGGAWPTEETLGMGAAEFTGAPTLNTFGGQFTTF
                     KLAPNQSIVVEIGDLFQETGVAGTTDELVYGEQYYFTAFGLDENGNAASDLSVTVSGT
                     TTASTNCTYTQGYWKNHEALWPVTNLTLGSVNYTAAQLDAILEEPVAGNGLVSLAHQL
                     IAAKLNVANGADPTAASAAIAAADALIGGLVVPPIGAGYLHPSVTSSLTQTLDDYNNG
                     LIGPGHCGNVPAEETTWGGVKALYR"
     gene            complement(3672..4217)
                     /locus_tag="VEC56_10420"
     CDS             complement(3672..4217)
                     /locus_tag="VEC56_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14604.1"
                     /translation="MCKRYLFVAASLVLMAAPVLSHANARLISVVPVGTGCVSGPTGP
                     AVQAWDVEPGETYTLTISHAVECANGGTDATMNVRVNSTTHGNTDIVATNVGVGIYEF
                     TYTMPTDAVCTFPIFYCTTPGAGNSGMFVIRNDGGMFQAHLRAASFGPGCTNPTKLEG
                     PECMTVPTDNKTWGQIKVLYR"
     gene            4509..6809
                     /locus_tag="VEC56_10425"
     CDS             4509..6809
                     /locus_tag="VEC56_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HXV14605.1"
                     /translation="MIIDVVGGGPAGLYFAIQMKLRDPAHRVTVVERNRRGSTFGWGV
                     VFSDGTLDRLRAADPATYARIADRFVHWDDIDVHYRGSVLRSGGHGFCGISRQALLDI
                     LSSRAEELGVGLRFESEIADEGALTGVDLVVAADGANSAIRKRHADRFRPDIDVRRCR
                     YVWLGTQKPFDAFTFYFEERPAGWYQAHCYPFGGGWSTFIVECPEETWRADGLDRASK
                     EETVAFCERLFARHLGQHRLVSNAEHLRGSAVWLNFPRVSCESWHTGNLVLLGDAAAT
                     AHFSVGSGTKLAMESAISLAEHLHEGGELDARLTAYERERRVEVLRLQNAARNSTEWF
                     EHVALKANLEPEQFNYSLVTRSQRVSHESLRARDPKYLESFERWLCARATGGARADSV
                     PPMFLPLRLREMTLANRVACSPMAMYSAVDGVVTDFHFVHFGERALGGAGLLFTEMTC
                     PSPDARITPGCAGLWTDAHAIAWKRIVDFVHANSDAKFALQLGHSGRKGSTKEPWLAD
                     GRDDAVLDAGNWPVLAPSPLPWGPQSQQPIEMSRADMERVRDEFVLATRRGIDAGFDA
                     LELHCAHGYLLSSFISPVTNVRRDAYGGSLANRLRYPIEVFEAMRAAWPKERPCGVRV
                     SATDWVEGGIAIADSVKIARAFRAAGADFIDVSSGQVTPEQKPVYGRMFQVPFAERIR
                     LELGIATLAVGNIYEPDHVNSIVAAGRADLCLLARPHLWDPYWTLRAAAQLGYQGVRW
                     PVQYLSGRKQIETLMQRARQSPAGPI"
     gene            6809..>7287
                     /locus_tag="VEC56_10430"
     CDS             6809..>7287
                     /locus_tag="VEC56_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV14606.1"
                     /translation="MEDTRDTAVLRDRHAIVTGAGSGIGAAIARALAGLGAHTTLIGR
                     RIEPLNALRRELESTTPGRPLARVADVTDAGLVARIVRDACAMQGAPFVLVNNAGAAF
                     SSAFLETDDAAWRAMLDANLMGAVFCLRAVLPLMRAQGEGRVVNMASTAGLEGYPYVS
                     "
BASE COUNT         1152 a         2442 c         2445 g         1248 t
ORIGIN      
        1 cttttatctc gcgcccgagg gcatcgtata cccgtgcctc acgattccat cgccgatggg
       61 ggacctcaag ggccgatcct tcgaggacct ctgggagtcg cccgaggccg acaccgttcg
      121 ccgccagatc gatgggtgcg agcagtgctg gatgatctgc acggctcgat cctcgctcaa
      181 gaagaacctg ccgcgcgcgc tgggctggat cgcgcgcgag aagttccgcg cgcacgcgca
      241 ctaagcgccg cgcggttggc cgtggtgttc gccgcttccg ccgatcccat cctctccggg
      301 tccacgccgc gtgtgcgcgg cgcggtcggc ttcctccgat gacgcgccgc accggcatgc
      361 gcccgctccg catcgccatg gtggggcaaa aggggatccc cgcccgctac ggcggtgtgg
      421 aaacgcacgt ggaaaacgtc gcggtgcgtc tggcggcgcg cggccacgac gtcaccgtgt
      481 tttgtcgccg gcgcttgcgc gccaacgggg acgcgcagga gacaccgagc cgcaacggaa
      541 cctatcgcgg tgtgcacctc ctctatcgcg caagcgtcaa cacgaagcac ttcgacgccg
      601 ctacccatgc ctttctttcg accctggagt cgtcctgccg gcaccacttc gacgtcgtgc
      661 acctgcacgg catcggcccg gccgcgttcg cgcccgtggc ggggctgttc ggacgcaaag
      721 tcgtctcgac gttccacgcg ctcgattggc gccaggtcaa gtggggaccg cgcgcgaagt
      781 cgctcttgcg gcgcggtgag gcgatcgggg ctcgggcaag cgacggtgtg atcgcggtct
      841 ccagactgat gcaggtccac atccagtcca cgcacggggt cgacgcggct tacattccca
      901 acggggccac gcttccggcg aagcccgtgt ctgcggggtc gctgtcgcaa tggggtctgg
      961 tcccgggcga atacctgctg acggtcgggc gcgtcatcgc cgaccgcgat ctggaaacgc
     1021 tgctgcgcgc cttcgcctcg attcgcgacc gccacccgcg tctgcgtctg gtaatcgtgg
     1081 ggtcggagac cccgcggacg gtctactcgg agttgctcga gcgcatggcg ggggagcgcg
     1141 tggtattcac cggcgatgtc ttcggcgaag cgttgcaggc gttgtacgcg cactgcctcg
     1201 tgtacgtgct ggcctcccgc gtcgaaggac tgcccatcac ggtatgcgag gcgatggccc
     1261 atgggcgcgc cctcgtcctg agcgacatcc ccgagaatgc ggaggtgggt ggggacaccg
     1321 cgcggttctt caagtgtggt gacgacagcg cgttggccaa tcagcttcac ccgttgatcg
     1381 aggaccgggc ggcgcgggat gccctgggcg cggtggcgcg cgcgcggtgc gaggcacact
     1441 acaattggga acacatcgcg gaccaggtgg agtcgttgta ctaccgggtt ctctcccacg
     1501 cctgacgtgg tgagcgcggg ccattttttg cttagcgccc aacggcggga gtgctaaggt
     1561 cgcgcgtcat gaagcgggct cttctcacca cgatgctggt cgcgacggcg gcggcgtggc
     1621 tctccggctg cgcgaccgta ccctcgtcga gcggcacacc gtcgcggccc gcgtcggtcg
     1681 agcgggcgag cgaccgcaac ccctacgact tccgatccga ggggaagatc ccacccctct
     1741 caccctccga cgcccccaac gaacccgacg tcgaggaaat gggcatcacg gagacggcca
     1801 tcgagtccag cgacgcggag gcgccacccg acacggcggc gcgcgctccc gagcccaccg
     1861 acacgctggt cgacggtttc cgcgtccagg tgttcgcgtc cgccgaccgc gagatcgcgg
     1921 agaacgcggc acgcggagcg caggagcggc tggggctcgc gagctacgtc gagttggacg
     1981 ggggcgtcta caaggtacgc gtgggggact acgcggcgcg cgcccaggcc gaccaggcct
     2041 tggccgcggt ccggcgccac tatgcggacg cctggatcgt cccgtcgaag gtacgcgccg
     2101 cccaacgtcc gtaagggcgg tccggagcgg cggttgacaa gtgtcctcgc acgggtataa
     2161 tgggtgtcgc caaacgactc gagagcggga atagctcagt ggtagagcac aaccttgcca
     2221 aggttggggt cgcgggttcg aaccccgttt cccgctccat tcttttctaa tccccaattg
     2281 cgttcgtagc ggacaatcgt cgtccgttca cgctggtccc gggtggcggc atagccaagt
     2341 ggtaaggcga cggtctgcaa aaccgttact ccccggttcg aatccgggtg ccgcctccaa
     2401 ctctcgaacc gcctcggccc gcgcaaacga gaagagcccg tcgctcgacg cgacgggctc
     2461 ttcgtgtact accggacccc tgacgggcca acgactaccg ataaagcgct ttgaccccac
     2521 cccaggtggt ctcttcggcg ggcacgttgc cgcagtgtcc cgggccgatg agaccgttgt
     2581 tatagtcgtc cagggtctgc gtgaggctgc tcgtgacact cgggtgcagg tatcccgccc
     2641 cgattggcgg cacgaccagg ccaccgatga gcgcgtcggc ggcagcgatg gccgcggacg
     2701 cggccgtcgg atccgcgccg ttggcgacgt tgagcttggc ggcgatcaac tggtgcgcga
     2761 ggctaaccag tccgttgccc gcgaccggtt cttccaagat ggcgtcgagc tgcgccgccg
     2821 tgtagttgac cgagcccaag gtgaggttgg tcaccggcca cagcgcttcg tggttcttcc
     2881 agtagccctg cgtgtacgtg cagttggtgc tcgcggtggt ggtaccgctg accgtgacgc
     2941 tcaagtcact ggccgcgttt ccattctcgt cgaggccgaa cgcggtgaag taatactgct
     3001 cgccgtacac gagctcgtcc gtcgtacccg cgacacccgt ctcctggaac agatccccga
     3061 tctcgacgac gatcgactga ttgggggcga gcttgaaggt cgtgaactgc ccgccgaagg
     3121 tgttcagcgt gggcgccccc gtgaactcgg cggcacccat ccccagggtc tcctcggtcg
     3181 gccacgcgcc gccgttggag agaaacgtgc tgcgatccat ccaccaaatg gcgaagccat
     3241 tggggaggcc gctcggaccg gccgtgatgg taatggcctg tttcgcgtgg ccggacgcgc
     3301 ccacgccgat gctgggctca tcgaccgccg ccaacgcggg caacgccaac acggccgcca
     3361 tcgccgccac gacgaacccc atccgatatc gtttggacat ctgattcctc catggaaggt
     3421 ttaattgcac ttcgcggtgt ggaggttctc ccgcaaaacg ccgcctccga cgccgaggcc
     3481 cgcctggctg tcatgttcac gtaaaagggc ccttgcttga gcgtagtcta gcacacgttc
     3541 ggccgaatcg gcacgcgcta ttcgcccggg agaggagtga tggcggtggg taagaaagtc
     3601 gggcccccgc gccgacgcac gggggcccgt tctttggata tcgggaactc ggtcggtaat
     3661 taccgtccgg gctaccgata gagaaccttg atctggcccc aggtcttgtt gtcggtcgga
     3721 accgtcatgc actccggacc ctcgagcttg gtcgggttgg tgcaccccgg accgaacgaa
     3781 gccgcgcgca ggtgcgcctg gaacatcccg ccatcgttgc ggatgacgaa catccccgaa
     3841 ttgccggccc ccggcgtggt gcagtagaaa atcgggaacg tgcacacggc gtcggtcggc
     3901 atcgtgtagg tgaactcgta gatgcccacg cccacgttgg tcgcgacgat gtccgtgttc
     3961 ccatgcgtcg tgctgttcac tcggacattc atggtggcgt cggtcccgcc gttggcacac
     4021 tcgaccgcgt ggctgatggt cagcgtatac gtctcaccgg gctcgacgtc ccacgcctgc
     4081 acggcggggc cggtcgggcc ggaaacgcac ccggtgccaa cgggcacgac gctgatcaag
     4141 cgcgcgttgg cgtgggacag aacaggcgcg gccatgagca cgaggctcgc ggccacgaat
     4201 agatagcgct tacacatttg gcctccaaaa actcccacag gtcgcggagg ctgcgttccg
     4261 cgtattggga ttcgtgatag gttacccgcg gtttgtcctt cgacctgccc ttcaaatcga
     4321 agaacgaatg agccatcaca tgcgctcagc gtgcgggggc tcccgcgaac tccacgacac
     4381 ccacactggc accgaccacg gccgcgggcc gggtcgcata cctttgaatt ggcgatagta
     4441 tactcccttt ctggggcccg tgtaaaggct cggttaaagg cgatgtttgt cgtatagtga
     4501 cgccggtcat gattatcgac gtcgtgggcg ggggcccggc cgggctctac ttcgccatcc
     4561 aaatgaagct ccgcgatccc gcccaccgcg tcaccgtggt cgaacggaac cgccggggca
     4621 gcaccttcgg ctggggagtc gtcttctcgg acggcaccct ggaccgcttg cgtgccgccg
     4681 atcccgccac ctacgcccgc atcgccgacc gcttcgtcca ctgggacgac atcgacgtcc
     4741 actaccgggg gagcgtgctt cgaagcgggg gccacggctt ctgcggcatc tcgcgccagg
     4801 ccctactcga tattctgtcg tcgcgggcgg aggaattggg cgtcggcctt cgtttcgagt
     4861 ccgagatcgc cgacgagggc gccctcacgg gcgtcgacct cgtggtcgcc gccgacggcg
     4921 ccaacagtgc cattcgcaag cgccacgctg accgctttcg ccccgacatc gacgttcgcc
     4981 gctgccgcta cgtatggctc ggcacccaga aacccttcga cgcgttcacc ttctatttcg
     5041 aagagcgccc tgccggctgg taccaggcgc actgctatcc cttcggcggg gggtggagca
     5101 cgtttattgt cgagtgcccg gaggagacgt ggcgcgccga tggtctcgac cgcgcgtcca
     5161 aagaagaaac cgtcgcgttc tgcgagcggc tgttcgcgcg gcacctgggc cagcaccgcc
     5221 tggtatcgaa tgccgagcac ctgcgcggat ccgcggtatg gctgaacttc ccgcgcgtta
     5281 gttgcgagtc gtggcatacg ggcaacctcg tgctcctggg ggacgcggcc gcgaccgccc
     5341 acttctcggt ggggtcggga accaaactcg cgatggagag tgcgatctcg ctcgccgaac
     5401 acctccacga gggcggcgaa ctcgacgcgc ggctgaccgc gtacgagcgc gagcgtcgcg
     5461 tcgaggtgct gcggctgcag aacgcggccc gcaacagcac ggagtggttc gaacacgtgg
     5521 ccctcaaggc caatttggag ccggagcaat tcaactatag cctggtaacg cgaagccagc
     5581 gggtgagcca cgagagcctg cgcgcacgcg atccgaagta cctggagtca ttcgagcgct
     5641 ggttgtgtgc gcgcgccacc ggcggcgcgc gcgcggactc ggtgccgccc atgttcctgc
     5701 ccctgcgcct gcgcgagatg acgctcgcca accgcgtcgc gtgctcgccc atggccatgt
     5761 acagcgcggt cgacggtgtg gtgaccgact tccacttcgt ccacttcggc gaacgcgcgc
     5821 tggggggcgc gggactcttg ttcaccgaga tgacgtgccc gtcgccggac gcgcgcatca
     5881 ccccggggtg cgcgggccta tggaccgacg cacacgcgat cgcgtggaag cgcatcgtcg
     5941 atttcgtgca cgcgaacagc gacgccaagt tcgcgctgca gctgggtcac tccggccgca
     6001 aggggtctac gaaagagccg tggctggccg acggacggga cgacgccgtg ctggacgcgg
     6061 gcaactggcc ggtcttggca ccctctccgc tcccgtgggg accacagagc cagcaaccca
     6121 tcgagatgtc acgcgccgat atggagcgcg tgcgcgatga gttcgtcctc gcgacgcgcc
     6181 gcggcatcga cgcgggcttc gacgcgctcg agttgcactg cgcgcacggc tatttgctgt
     6241 cgagtttcat ctcgcccgtc accaacgtgc gtcgggacgc ctacggcggc tcgctcgcga
     6301 accgcctgcg ctatccgatc gaagtgttcg aggcgatgcg cgcggcctgg ccgaaagagc
     6361 ggccctgcgg cgtgcgcgtg tccgccaccg actgggtcga aggcggcatt gcgatcgcgg
     6421 actcggttaa gatcgcgcgc gcgtttcgcg ccgcgggcgc cgatttcatc gacgtgtctt
     6481 cgggacaggt gacgccggag cagaagccgg tctacgggcg catgtttcag gtccccttcg
     6541 ccgagcgcat ccgactggag ctgggcatcg cgacccttgc ggtgggcaac atctacgaac
     6601 ccgaccacgt gaacagcatc gtggcggcgg ggcgcgcgga tctgtgcttg ctcgcgcggc
     6661 cccacctgtg ggatccctac tggacgctgc gcgcggccgc ccagctcggc taccaaggcg
     6721 tacgctggcc ggtccagtac ctctcgggcc gcaagcaaat cgaaaccctg atgcagcggg
     6781 cgcggcagtc gccggcgggg ccgatctaat ggaagacacg cgagacaccg ccgtgctgcg
     6841 cgatcgccat gcgatcgtca ccggtgccgg cagcggcatc ggcgccgcga ttgcgcgcgc
     6901 gctcgcgggc ctgggcgccc acacgaccct catcggacgc cgcatcgagc cgctgaacgc
     6961 cttgcggcgc gagctcgagt cgaccacccc gggaaggccg ctggcgcgcg tcgccgacgt
     7021 caccgatgcc ggcttggtgg cgcgcattgt tcgtgacgcg tgtgcgatgc aaggcgctcc
     7081 gtttgtcctc gtcaacaacg cgggcgcggc gttttcgagc gcgttcctcg agaccgacga
     7141 cgcggcctgg cgggccatgc tcgacgccaa cctgatgggt gcggtattct gcctgcgcgc
     7201 ggtcctgccg ctgatgcgcg cgcagggcga ggggcgcgtc gtcaacatgg cgagcacggc
     7261 gggcctcgaa gggtatccgt acgtgtc
//
LOCUS       DASPGK010000377         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_444065, whole genome shotgun sequence.
ACCESSION   DASPGK010000377 DASPGK010000000
VERSION     DASPGK010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444065"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="VEC56_10435"
     CDS             complement(<1..1752)
                     /locus_tag="VEC56_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14607.1"
                     /translation="MTPRAALPSDRAMRVALAVLAFMVRLPLLANADFVSYDGTYYIN
                     QAQALLGGSLAGGAFPIGYPLLIAPVLAIVRDGVVAGAWVSLLASIGSVLVFEALCRR
                     YVRRPLALLAAVVFAITPLFMRASVLTLSESAYTFWVLLSLLQFEKRPWGSGLAIGMA
                     AATRPEALAVAGGLGMVAALRFLFRRPGSGPRALAAFLLGFGVVYGASVAAMSVAHGR
                     VTLLSRAGAYRSVAMPWQLRETSLDYEGRERVESAFERDRPAFDRRASYGEVMGEEIA
                     GLSRQLMYLVPALGIVGVVAHRGFVAVALVPLLFIPFFTEARGQVRWLVPYLAPLILY
                     AFRVVDAARTSRARFATAALLAALSLASLWINRSVFATGVESEYESTRGVARRFAGRI
                     EPGDAIADRKPYFAFYAGARYVEIPAAPYDDTVAYLANEGVRYLALHAKSVDRLRPAL
                     RPLVFDAAAVRGELRYRQIVQEPTGELIYERTGTTDSLSFRRLTRPEIGDLTPAWSPD
                     GRRIALRRFLPDGGAALVLVDRDGGNERELARTSRERDPIAWSPDGARIVYTTLAGGH
                     FDVVSLDVRSGATARIAG"
     gene            complement(1749..>3355)
                     /locus_tag="VEC56_10440"
     CDS             complement(1749..>3355)
                     /locus_tag="VEC56_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14608.1"
                     /translation="TIPEKDAMDHDGGYATRLPRTLLSLGRQLLPLIPFLALVGMVRK
                     PNFVLAMLAPLPLLPLFMLERDQTRWALLTPYLPPLIFYAMVALDAVRRPRARVIATW
                     AVVVSAGVCFWINRSLLQRGPESDFDTARAVGRRLASSVAPGDMMADRKPYLALYSGA
                     RYVEIPVAPYDDTVGHLAKAGVRYLSLHPKSVALRPALLPLVFDASAVRGEIRYRQVL
                     VEPTGEMVLERARADDPLSQRPLTQGESVDFAPAWSPDGSRVAFRRVAPDGTGAIWLV
                     DASGANARELARTSVERDRLAWSPDGHRIAYASMTDRQLDLFAVDVATGRVTALFESS
                     DFEWSPSWNRASGALVFCGDPGGSPSVYMLEANRTAVRLSESEPTDLASVSPSGRFVT
                     WVDLEGRLVVFEVNARTRRVVDEPKQILSAASWSPDERYVVVEAYDWGSSNLYIIDVH
                     ARRAMMLTKAITGEGMPSWSPKGDEIVCLFDRDGAVGLSVVGNFSEYVRLFAQGDDVD
                     VFDRPQETRVAPREQLRRVRAETRRP"
BASE COUNT          593 a         1194 c         1148 g          420 t
ORIGIN      
        1 gccggcgatg cgcgcggtcg cgcccgagcg cacgtccagc gagacgacgt cgaagtggcc
       61 gccggccagg gtcgtgtaca cgatgcgcgc tccatccggc gaccacgcga tcgggtcgcg
      121 ctcgcgtgac gtccgtgcca gctcgcgctc gttgccccca tcgcgatcga cgaggacgag
      181 ggccgcgccg ccgtcgggga gaaagcggcg cagcgcgatg cggcgcccgt ccggcgacca
      241 cgccggcgtg aggtcgccga tctcgggccg cgtcagccgg cgaaacgaca gcgagtcggt
      301 ggtaccggtt cgctcgtaga tcagctcgcc ggtcggctcc tgcacgatct ggcggtagcg
      361 cagctcgccg cgcaccgcgg cggcgtcgaa cacgagcggc cgcagcgcgg ggcgcaatcg
      421 atcgacggac tttgcgtgca gggcgaggta gcgcacgccc tcgttcgcca ggtacgcgac
      481 agtgtcgtcg tacggcgcgg ccgggatctc gacgtagcgc gcgcccgcat agaacgcgaa
      541 gtaaggcttg cgatccgcga tggcgtcgcc gggttcgatt cggcccgcga agcgtcgcgc
      601 gacgccgcgc gtactctcgt actcggactc gaccccggtc gcgaagacgg agcggttgat
      661 ccatagcgac gccagtgaca gcgccgccaa gagcgccgcg gttgcgaacc gtgctcgcga
      721 cgttctcgcc gcgtccacca cgcgaaaggc atatagaatg aggggcgcga ggtacggcac
      781 cagccagcgt acctgtccgc gcgcctcggt gaagaacgga atgaacagga gcgggaccag
      841 cgcgacggcg acgaagcccc ggtgcgccac cacaccgacg atcccgagcg cgggcacgag
      901 gtacatcaac tgccgggaca gccctgcgat ctcttcgccc atcacctcgc cgtaggaggc
      961 acggcgatcg aacgcggggc gatcgcgctc gaacgcggac tcgacgcgct cgcggccttc
     1021 gtagtcgagc gaagtctccc gcagctgcca cggcatcgcc accgaccggt acgcaccggc
     1081 gcgagagagc agcgtgacgc gaccgtgcgc gaccgacatc gccgccacgc tcgcgccgta
     1141 cacgaccccg aagccgagca ggaacgcggc cagcgcgcgc gggccggagc ccgggcgccg
     1201 gaacaggaac cgtaacgcgg ccaccatgcc cagcccgccc gctaccgcga gcgcctcggg
     1261 ccgcgtcgcc gccgccatcc cgatcgccaa accggaaccc cacggtcgtt tctcgaactg
     1321 gagcagagag agaagcaccc agaacgtgta cgccgattcg gagagcgtca ggacggacgc
     1381 gcgcatgaac aacggcgtga tcgcgaacac gaccgccgcg aggagcgcca gcggccgccg
     1441 cacgtaccgg cgacacagcg cttcgaagac gaggacggac ccgatcgacg ccagcagcga
     1501 gacccacgct cccgccacca ccccgtcgcg cacgatcgcc agtaccggcg cgatcagcag
     1561 cggatacccg atgggaaacg cgcccccggc gagcgacccg ccgagcaggg cctgggcctg
     1621 attgatgtag taggtgccgt cgtacgagac gaagtcggcg ttggccagga gcgggagccg
     1681 aaccatgaac gcgaggacgg ccagtgccac gcgcatcgcg cgatcggacg gcaacgcggc
     1741 gcgcggggtc acggtcgtcg cgtctcggcg cgcacgcggc gcagttgttc gcgcggtgcc
     1801 acgcgcgtct cctggggacg atcgaacaca tcgacgtcgt ccccttgcgc aaagagacgc
     1861 acgtactccg agaagttgcc gaccaccgac aggcccacgg cgccgtcgcg gtcgaacagg
     1921 cacacgatct cgtcgccctt ggggctccag ctcggcatgc cttcgcccgt gatcgccttg
     1981 gtgagcatca tcgcgcgacg cgcgtggacg tcgatgatgt acaggttgga cgatccccag
     2041 tcgtacgctt cgaccacgac gtagcgctcg tcggggctcc acgacgcggc cgacaggatc
     2101 tgcttcggct cgtcgacgac gcgcctcgtg cgcgcattca cctcgaagac gaccaggcgt
     2161 ccttcgaggt cgacccatgt cacgaaccgg cccgagggcg acacgctggc caggtccgtg
     2221 ggttcgctct ccgacaaacg caccgccgtg cggttcgctt cgagcatgta caccgacggc
     2281 gatccgccgg gatcgccgca gaaaacgagc gcccccgacg cgcggttcca ggacggactc
     2341 cactcgaagt cgctcgactc gaacagcgcg gtcacgcgac cggtcgcgac gtccaccgcg
     2401 aagagatcga gctggcgatc ggtcatggac gcataggcga tacgatgtcc gtccggcgac
     2461 catgccagcc ggtcgcgctc cacgctggtg cgcgcgagct cgcgcgcgtt cgcccccgac
     2521 gcgtcgacca gccagatggc cccggttccg tccggcgcga cgcggcgaaa cgcgacccgg
     2581 ctcccgtcgg gcgaccacgc gggtgcgaag tcgacggact ccccctgggt caggggacgc
     2641 tgcgagagcg gatcgtccgc gcgtgcgcgt tccaagacca tctcacccgt cggctccacc
     2701 aatacctggc gatagcggat ctcgccgcgc acggccgacg cgtcgaacac cagcggcagc
     2761 agcgccggtc gcagggcgac gctcttcgga tggagcgaga ggtacctcac tcccgccttc
     2821 gcgagatggc cgacggtgtc gtcgtagggt gcgactggaa tctcgacgta gcgggcgccc
     2881 gagtagagcg cgagataggg cttgcggtcg gccatcatgt cgccgggcgc cacactcgac
     2941 gccaatcggc gcccgaccgc gcgcgcggtg tcgaaatcgc tctcgggccc gcgctggagc
     3001 aacgatcggt tgatccagaa gcacacgccg gcggacacga cgaccgccca ggtcgctatg
     3061 acgcgcgcgc gcggtcgtcg taccgcgtcc agcgctacca tcgcgtagaa gatcaagggc
     3121 ggcaaatagg gtgtcaacag cgcccagcgc gtctggtcgc gctcgagcat gaacagcggc
     3181 agcagcggga gcggcgccag catggccagc acgaagttcg gcttgcgcac cattcccacc
     3241 agtgcgagaa acggaatcag cggaagcagc tggcgaccga gcgagagcag cgtgcgcggc
     3301 aggcgcgtcg cgtaaccccc gtcgtggtcc atcgcgtcct tttccggaat cgtct
//
LOCUS       DASPGK010000378         5947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_560655, whole genome shotgun sequence.
ACCESSION   DASPGK010000378 DASPGK010000000
VERSION     DASPGK010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560655"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VEC56_10445"
     CDS             complement(<1..1113)
                     /locus_tag="VEC56_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HXV14609.1"
                     /translation="MTQKLYLEEPYQTAFEATVVSSRALDAGTREVVLDRTLFYPESG
                     GQLADAGVLGEARVTGVREGDADAVVHVVAGDVPAAGEVAGTIDWARRFDHMQQHTGQ
                     HVLSRAFIETGGLPTVSFHMGEETCTIDLEGSGFDDAAARRAEDLANRIVAENRPIAV
                     SLVPLAELDQLGLRRKVPEGVTIARIVSVADFDAIPCCGTHVRSTGELALVKVLRAER
                     VKNLSRVHFKVGARALDDYREKHGIVQALANRLTTSAGDVLHKVERMTEESQVASKRA
                     KQLVQRLAEAHKPALLQRAVAAGAHRVVVYRDADAALVRALGTELQSESGTVALLGAD
                     DGTVVCVTARDVTLDLAGAASAMARELGGSGGGKGGF"
     gene            complement(1169..1534)
                     /gene="panD"
                     /locus_tag="VEC56_10450"
     CDS             complement(1169..1534)
                     /gene="panD"
                     /locus_tag="VEC56_10450"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HXV14610.1"
                     /translation="MELLLLKSKIHQATVTGIDMHYEGSIVIDAALMEEVGLTRWEKV
                     LVANMNTGDRFETYAIPAPARSGRIELNGATARLGHIGDRVIIFSFATYTEAEARTHA
                     PKIVQLDDENRVIRRSFVA"
     gene            complement(1612..3162)
                     /gene="lnt"
                     /locus_tag="VEC56_10455"
     CDS             complement(1612..3162)
                     /gene="lnt"
                     /locus_tag="VEC56_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXV14611.1"
                     /translation="MKLLPGRVLPPLPLVLPALLSGVLLTLCFPVASLGPVSFLALTP
                     VFAAVLRHRPGRKDAFRAGFLTGSACFVSMLWWIVKLVPTAGVTIPWLMTPALIVLVL
                     YLSVYPAFHLLVVAAVSRWRLIPFVLSAPATWALMEIARSRGELAFPWGLIGYSLSDH
                     PALLQTADVWGVFGLSFVVVLVNALLAGSATARGPRAKAWCGLFAGLIAGSTWLYGSA
                     RVERERALDEPTLRVALAQPNVDLALKWKPEFKDSTFALIEKQAAQARDSGAVLVIFP
                     ETAAPVYIENSPVNKMRLIELARGLGIPFYIGFLDHRFDGPDKTLNIYNSSGLFLPDG
                     SLEKYDKRHLLPFGEALPLATRYRWIRKIEFGQANFEPGPVRSPLTGDGVTFTPLICF
                     ESVFPDLCRDGVREGSELFVNITNDGWFGDTPGPYQHAQMAIVRAVEFRRWLVRSANS
                     GVSMVVAPTGEVVSSIGLYKEDILMADVALRSGATFYARHGEAPLVWMSLVLVGVAAW
                     LSRRASMA"
     gene            complement(3200..4246)
                     /locus_tag="VEC56_10460"
     CDS             complement(3200..4246)
                     /locus_tag="VEC56_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV14612.1"
                     /translation="MRAPCVTMPAMADKTRIAIVGAGHVAQVAHIPAYRANPDVELVA
                     VVDEDPVKARRIKEQAGFKVWYEDFSEMLKKADVDAVDICTPNYLHAPMTIAALRSGR
                     DVMCEKPLARNAAEAQRMVDTAEEHGRILMVAMNKRFREDAQVLQKFITGNELGDIHV
                     IKAGWLRRATDWKDRAWFTERGKAGGGALLDLGTPLVDLAIWISGLKKITGVSCSVFG
                     KKGRDGVEDSGCALVRFAGGACLMLEVNWNLRDPRDVVYLQVYGSKGAGILTPLQVHK
                     SIQGVLVNVTPALGKQKNFYKESYQAEIDHFIQCVRKQRAPVVTGKDAVAVLRIVDAM
                     YESASSGHEVDLAG"
     gene            complement(4250..5245)
                     /locus_tag="VEC56_10465"
     CDS             complement(4250..5245)
                     /locus_tag="VEC56_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV14613.1"
                     /translation="MKSPHPCFDPPIVGSSAAAQCLRDLVLTLADSSVSVLITGDSGV
                     GKEVLARNLHRFSPRAHAAFVSVNCAALAPGILESELFGHDRGAFTGAVRAHAGLFEQ
                     ASGGTLFLDEIGEIPPYIQAKLLRVLQEGEVRRMGEGGVRRVDVRLLSATNANLAHMV
                     GDGSFRKDLFYRINVVEARVPSLRERREDVVDLVAHFFERRGLARPGVPAETARILSS
                     YPWPGNVRELENEVERIAALHGTPARLGPEMLSERVLCAAAAATLDVAVLHEAPLASA
                     VGHLEESLLKKTLAQTNWNKSHAARRLGLSRQGLLKKIKRYGIERGAEFDKENRA"
     gene            complement(5470..>5947)
                     /locus_tag="VEC56_10470"
     CDS             complement(5470..>5947)
                     /locus_tag="VEC56_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14614.1"
                     /translation="RVRVEGTLSLRGSTPVTTSVLEIDSTEAIPLDSKKPGLLTQLKG
                     LSGMRCAIEGEVLPFVDRNLPRLSATRYELLPLPDGRVPIIGIASLEDGQCVVTTDDG
                     KRYWIHGELVGVLNEYAGARVWVAGDVFDSDAKARPKKSTPLTATGYGVVDEAPAR"
BASE COUNT          994 a         2017 c         2004 g          932 t
ORIGIN      
        1 gaagccgccc ttgccgccac ccgagccgcc caattcgcgc gccatcgcgg acgccgcgcc
       61 cgcgaggtcc agggtgacgt cgcgcgcggt gacgcacacc accgtgccgt cgtcggcgcc
      121 gagcagcgcc accgtccccg actccgattg cagctcggtg ccgagcgccc gcaccagcgc
      181 cgcgtccgcg tcccggtata ccaccacgcg gtgcgcgccg gcggcgaccg cgcgctgcaa
      241 cagcgcgggc ttgtgcgcct cggccagacg ctggaccagc tgcttcgcgc gcttgctcgc
      301 cacttgcgac tcttcggtca tgcgctcgac tttgtgcagc acatcgccgg ccgaggtcgt
      361 gagccggttg gcgagcgcct gcacgatgcc gtgcttctcc cgataatcgt cgagagcgcg
      421 tgcgcccacc ttgaagtgca cgcgactgag attcttcacg cgctccgcgc gcaagacttt
      481 gaccagcgcc agttcgccgg tcgaacgaac gtgcgttccg cagcacggaa tcgcgtcgaa
      541 gtcggccacc gagacgatgc gcgcgatggt cacgccttcc ggcaccttgc ggcgcagccc
      601 gagctggtcc agctcggcga gcggaaccag gctcaccgcg atggggcggt tctcggcgac
      661 gatacgattg gcgaggtcct ccgcgcgccg cgcggccgcg tcgtcgaacc cggagccctc
      721 gaggtcgatg gtgcaggttt cctcgcccat gtggaacgaa accgtcggca agcccccggt
      781 ctcgatgaac gcccgcgata gaacgtgctg cccggtgtgc tgctgcatgt ggtcgaaacg
      841 gcgcgcccag tcgatggtac ccgccacctc gcccgccgcg gggacgtcgc ccgccacgac
      901 gtgcacgacc gcgtccgcgt cgccttcgcg cacgccggtg acgcgcgcct caccgagcac
      961 gcccgcgtcg gccaactgcc cgcccgattc cgggtagaac agagtgcggt cgaggacgac
     1021 ctcgcgggtt cccgcgtcga gcgcgcggga cgagaccacg gtggcctcga acgccgtctg
     1081 gtagggctct tcgagataca gcttctgcgt catgaaacga tgcgcgaacg agtcggcgga
     1141 aacgaccgac gatcgaacga tgggatcgct aagcgacgaa ggaacggcgg atcacgcgat
     1201 tctcgtcgtc gagctggacg atcttgggcg cgtgcgtcct cgcctccgct tcggtgtagg
     1261 tggcgaagga gaagatgatc acgcggtcgc cgatgtggcc caggcgcgcc gtcgccccgt
     1321 tcaactcgat gcgtccggaa cgggccggag cggggatcgc gtacgtctcg aaacgatcgc
     1381 ccgtgttcat gttggcgacg agcactttct cccaccgcgt gagcccgact tcctccatga
     1441 gggcggcgtc gatgacaatg ctaccctcgt agtgcatgtc gatgccggtg acggtggctt
     1501 gatgtatctt cgatttcaag agaaggagtt ccatcgcgac ctatcataac gcggccggag
     1561 accgggcaca agggagcgct ggggaatacg tgggaagcgg gcgggacgcg ctcacgccat
     1621 cgacgcgcgg cgcgagagcc acgcggccac gcccactaga acgagggaca tccataccag
     1681 gggcgcctcg ccgtggcgcg catagaaggt cgctcccgag cgcagcgcga cgtcggccat
     1741 caggatgtcc tccttataaa ggccgatcga cgacaccacc tccccggtcg gcgccaccac
     1801 catcgagacg ccgctgttgg cgcttcgaac cagccagcgc cggaactcca ccgcgcgcac
     1861 gatcgccatc tgtgcgtgct ggtacggacc cggtgtgtcc ccgaaccaac cgtcgttggt
     1921 gatgttgacg aagagctcgg agccctcgcg cacgccgtcg cggcacaggt ccgggaacac
     1981 cgactcgaag cagatcaacg gcgtgaaagt gacgccgtcg cccgtgagcg gcgagcgcac
     2041 cgggccgggc tcgaaattgg cctggccgaa ttcgatcttg cgaatccacc ggtagcgcgt
     2101 ggcgagcgga agcgcttcgc cgaacggcag gaggtggcgc ttgtcgtact tctcgagcga
     2161 cccgtcgggg agaaacaaac ccgacgaatt gtagatgttg agcgttttgt cgggaccgtc
     2221 gaagcgatgg tccaagaagc cgatgtaaaa ggggatcccc agcccgcggg cgagttcgat
     2281 taggcgcatc ttgttgacgg ggctgttctc gatgtaaacc ggcgccgccg tctcgggaaa
     2341 aatcaccaac accgcaccgc tgtcgcgcgc ttgcgcggct tgcttctcga tcagcgcgaa
     2401 ggtggagtcc ttgaactccg gcttccactt gagcgccaag tccacgttgg gctgcgcgag
     2461 cgcgacgcgc agcgtcggct cgtcgagtgc gcgctcgcgc tcgacgcgcg cggatccgta
     2521 caaccacgtc gaccccgcga tcaatcccgc gaagaggccg caccacgcct tggcgcgcgg
     2581 cccgcgcgcc gtcgccgacc ccgccaggag agcgttgacc agtaccacga cgaacgacaa
     2641 gccgaacacg ccccacacgt cggcggtctg caggagcgcg gggtggtccg agagcgagta
     2701 gccgatcagg ccccagggga acgccagctc gccgcgcgag cgcgcgatct ccatgagcgc
     2761 ccaggtcgcg ggggcggaca gcacgaacgg gatcaatcgc cagcgcgaga ccgccgcaac
     2821 caccagcagg tgaaacgcgg ggtacaccga gaggtaaagc accagtacga tgagtgcggg
     2881 agtcatgagc caggggatag tgacgccggc ggtcggcacc agtttcacga tccaccacag
     2941 catcgacacg aagcacgccg agcccgtgag gaagccggcg cgaaacgcgt ctttgcgccc
     3001 cggccggtgg cggagcacgg cggcgaacac cggcgtgagc gcgagaaagg acaccggccc
     3061 cagcgacgcc accggaaaac acagcgtcag cagcacgccg gacagcagcg ctgggagcac
     3121 gaggggaagc gggggcagga ccctgcccgg caggagtttc atcgcggggt gagacgcatc
     3181 tcggagtcgc gcgtcgcgct catccggcca ggtcgacttc gtggcccgac gacgccgact
     3241 cgtacatggc gtcgacgata cgcaggacgg cgacggcgtc cttgcccgtt accaccggcg
     3301 cgcgctgctt acgaacgcac tggatgaagt ggtcgatctc ggcttgatag ctctctttgt
     3361 agaaattctt ctgcttgccg agcgcgggtg tcacgttgac caatacgccc tggatcgact
     3421 tgtgtacctg caggggcgtc agtattcccg cccctttgct cccgtacact tgcagataca
     3481 ccacgtcgcg cgggtcgcgc aaattccagt tcacttccaa catgaggcag gccccgcccg
     3541 cgaagcggac cagcgcgcac cccgagtctt cgaccccgtc gcggcccttc ttgccgaaca
     3601 cgctgcacga cacgccggtg atcttcttga gcccggagat ccagatggcc aggtcgacca
     3661 gcggcgtccc caggtcgagc aacgcgccgc cacccgcttt accgcgctcg gtgaaccacg
     3721 cgcgatcctt ccaatcggtg gcgcggcgca gccagcccgc cttgatgacg tggatgtcgc
     3781 ccagctcgtt gccggtgatg aacttctgca gcacctgggc gtcctcgcgg aagcgtttgt
     3841 tcatcgccac catgaggatg cgtccgtgct cctcggcggt gtcgaccatg cgctgcgctt
     3901 cggcggcgtt gcgtgccagc ggcttctcgc acatgacgtc gcgccccgaa cgcagcgccg
     3961 cgatggtcat gggagcgtgg aggtagttgg gcgtgcagat gtcgacggcg tccacgtccg
     4021 ccttcttcag catctcggag aagtcttcgt accagacctt gaagccggcc tgttctttga
     4081 tgcggcgtgc ctttacgggg tcttcgtcga cgacggccac caactcgacg tcggggttgg
     4141 cgcgataggc gggaatgtgg gcgacctggg cgacgtgccc ggcgcccacg atcgcgatgc
     4201 gggtcttgtc ggccatcgcg ggcatggtaa cgcagggggc tcgcacttgt cacgccctgt
     4261 tctccttatc gaactccgct ccccgctcga tcccgtagcg cttgatcttc ttcaggagcc
     4321 cctggcgcga aaggcccagc cggcgcgccg cgtgcgactt gttccagttg gtctgcgcga
     4381 gcgtcttctt gaggaggctt tcctccaggt ggccgaccgc gctcgcgagc ggggcctcgt
     4441 gcagcacggc gacgtccagg gtcgcggccg cggccgcgca cagaacgcgc tccgagagca
     4501 tctcgggccc caggcgcgcg ggggtaccgt ggagcgccgc gatgcgctcg acttcgttct
     4561 cgagctcgcg cacgttgccg ggccacggat agctcgaaag aatgcgcgcc gtctccgcgg
     4621 gtacgccggg gcgagcgagc ccgcgtcgct cgaagaagtg cgccaccaaa tcgaccacgt
     4681 cttcgcgccg ctcgcgcagc gacggtacgc gcgcctccac gacgttgatg cgatagaaca
     4741 agtcttttcg aaacgagccg tcgccgacca tgtgggcgag gttggcgttg gtggcgctga
     4801 gcaaccggac gtccacgcgc cgaacgcccc cctctcccat gcggcgcact tccccttcct
     4861 gcaagacgcg caggagtttg gcctggatat agggagggat ctcgccgatc tcgtccagga
     4921 acagcgtccc gccgctcgcc tgctcgaaga ggccggcgtg cgcgcgcacg gcacccgtga
     4981 acgcgccgcg atcgtggccg aagagctcgc tctcgaggat gccgggcgcg agtgcggcgc
     5041 agttgaccga cacgaaggcg gcgtgcgcgc gcgggctgaa gcgatggaga ttgcgcgcga
     5101 ggacttcttt gcccactccg ctatcgccgg tgatgaggac gctgacgctg gagtcggcga
     5161 gggtgagcac gaggtcgcgc aggcactggg ccgcggccga agaacccacg atgggcgggt
     5221 cgaagcacgg gtggggcgat ttcacgcggg ccggagacgg caagcccggt gccacgggtt
     5281 tcgtcgcgag gccaacttcg cgtgcgcgag ggactttcat cgccgcggcg ttgccgccgc
     5341 cccggcgccc ggcgcgcatc catacccgtc acgcgttcgc gcgtcgcgtc ggtagcacgc
     5401 gaccgcgacg acgcttgcgg agattcttgg agggcaaatg cgctgtcgac gattcgacag
     5461 cgggcctggt cagcgcgcgg gcgcctcgtc gacgacgccg tagccggtcg ctgtcaacgg
     5521 cgtggacttc ttgggccgcg ccttggcgtc ggaatcgaac acgtcgccgg cgacccacac
     5581 gcgcgctccc gcgtactcgt tcaagacgcc gaccaactcg ccgtggatcc agtagcgctt
     5641 cccgtcgtcg gtcgtgacga cgcactggcc gtcttcgagc gacgcgatgc cgatgatggg
     5701 cacgcgaccg tcgggcagcg ggagcagctc gtagcgcgtc gcggacaagc ggggaaggtt
     5761 gcgatcgacg aacgggagca cctcgccctc gatcgcgcag cgcattcccg acaacccctt
     5821 gagttgtgtg aggagcccgg gcttcttgga gtcgagcggg atcgcttcgg tgctgtcgat
     5881 ctcgagcacg ctggtggtaa cgggcgtgct gcctcgcagc gacaacgtgc cttcgacgcg
     5941 tacgcgc
//
LOCUS       DASPGK010000379         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_678156, whole genome shotgun sequence.
ACCESSION   DASPGK010000379 DASPGK010000000
VERSION     DASPGK010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678156"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VEC56_10475"
     CDS             complement(<1..881)
                     /locus_tag="VEC56_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXV14615.1"
                     /translation="MSLFSKLSNYLTNDVAIDLGTANTLVFVKGNGIVLNEPSVVAVD
                     QRTKKVYAVGLEAKTMLGKTPEYINAIRPMKDGVIADFEITEMMLREFIRKSQKKKHF
                     IRPRIVIAVPSGITEVEKRAVRDSAEHAGAREVFLIAEPIAAAIGVGLPVDKPSGNMI
                     IDIGGGTTEIAVIALDGIVADSSIRVAGDELDEAIVNYVKRTYNMLIGEQTAEQIKMK
                     IGCAVKMDKPYEMEIKGRDLVAGIPKNLRLTSEEIREAISEPISQIVEALKNALEQTP
                     PELAADIVDRGIVMTGGG"
     gene            complement(906..1589)
                     /gene="radC"
                     /locus_tag="VEC56_10480"
     CDS             complement(906..1589)
                     /gene="radC"
                     /locus_tag="VEC56_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXV14616.1"
                     /translation="MGGRGRIGIPSSDRPRERLYTRGAAELSLQELLAIVVGGGSRGS
                     SALVLALGLLREFGDLVVMGRSGVDEMRRVPGIGFARACQLAAAFELGKRFAKESRSA
                     GTSVRAPRDIARMFMDEMKDYDREHFKAAFLNTKNQVIKVVTVSIGSLNASIVHPREI
                     LKPAIAASAASIVLVHNHPTGDPTPSREDVEFTRRFAKCGELIGIELLDHIVIGADRF
                     QSLKEAGHF"
     gene            complement(1705..>2873)
                     /locus_tag="VEC56_10485"
     CDS             complement(1705..>2873)
                     /locus_tag="VEC56_10485"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV14617.1"
                     /translation="PAITIACTALCVGVFVLHKFFAGSVALEFRNYIYFPGYSNLGPA
                     LAAAFLHFDYLHIVTNLVYLVLFGRYLEDRLGPAGFALVFLGAAVVGNVLQGWYNLHV
                     LETNAGIIGASGGVSGVLGAFLVRLRHHRVKIAYWVFAPLLATNKAGKSELHAVAAIG
                     LWILIQLVRGLVQLEGASVNVAYVTHIAGFGFGVFVAIASGGWAKGRVDGHLLKARRC
                     LRRGDFWGANDELEHYAAARPSDGEAHALLARVRAQCGDRAGARASYASACENLLAAG
                     RRGEAERIYQEASRSLPEFVLEPERQLDLCFGLERNLKPDVAIRAYEGFVQVHPNHPE
                     AAFALLRAANLHAKRGDLSRALHCYEIIPAEYPDDPWAEFAAEHARRLTLATQG"
BASE COUNT          505 a          961 c          919 g          488 t
ORIGIN      
        1 cccccgccgg tcatgacgat gccgcgatcc acgatgtcgg cggccaactc gggcggggtc
       61 tgctcgagcg cgtttttcaa ggcctccacg atctgcgaga tcggctcgct gatggcctcg
      121 cggatctcct cgctggtcaa gcgcaggttc ttggggatgc cggcgacgag atcgcgcccc
      181 ttgatctcca tctcgtaggg cttgtccatc ttgaccgcgc acccgatctt catcttgatc
      241 tgctcggcgg tctgctcgcc gatcagcatg ttgtacgtgc gcttgacgta gttgacgatg
      301 gcttcgtcca gctcgtctcc cgcgacacga atcgagctgt cggccacaat gccgtccagc
      361 gcgatgaccg cgatctccgt cgtgccgccg ccgatgtcga tgatcatgtt gccggagggc
      421 ttgtcgaccg ggaggcccac gccgatggcg gccgcgatcg gttcggcgat caggaacacc
      481 tcgcgcgcgc ccgcgtgctc ggcggagtcg cgcaccgcgc gcttctccac ctcggtgatg
      541 ccggacggca ccgcgatgac gatgcgcggg cgaatgaaat gtttcttctt ctgcgatttc
      601 cgaatgaact cgcgcagcat catctcggtg atctcgaagt cggcgatgac gccgtccttc
      661 atgggccgga tggcattgat gtactcgggc gtcttgccga gcatggtctt ggcctcgagg
      721 ccgaccgcgt acaccttctt ggtacgctgg tcgacggcga ccacggacgg ctcgttcaac
      781 acgatgccgt tgcccttgac gaacaccagg gtgttcgcgg ttccgaggtc gatggcgacg
      841 tcgttcgtga ggtaattcga cagcttggaa aacagcgaca ttccgtaaac tcccgggcgt
      901 gacgactaga agtggcccgc ttccttcaga ctctggaacc gatccgcgcc gatgacgatg
      961 tggtcgagca actcgatacc gatcaactct ccgcactttg cgaagcgacg ggtgaactcg
     1021 acgtcctcgc ggctcggcgt cggatcgccc gtcgggtgat tgtgaaccaa aacgatggat
     1081 gcggcactcg cggcgatcgc gggcttcaag atctcgcgcg ggtggacgat ggatgcgttg
     1141 agcgatccga tcgacaccgt cacgaccttg atgacctgat tcttcgtgtt caggaacgcc
     1201 gccttgaaat gctcgcgatc ataatccttc atttcgtcca tgaacatgcg tgcgatgtcc
     1261 cgcggcgcgc gcacggaggt cccggccgaa cgcgattcct tggcgaagcg cttgcccagc
     1321 tcgaaggcgg ccgccagttg acacgcccgg gcgaacccga ttccgggtac ccggcgcatc
     1381 tcgtcgacgc cggagcggcc catcaccacc agatccccga attcccgaag caatcctagc
     1441 gccagtacca aggcactcga cccgcgcgag cctcccccga cgacgatcgc aagaagttct
     1501 tgcagagaaa gctcggccgc gccgcgcgta tacagccgct cacgcggtcg gtcgctcgac
     1561 ggaatcccaa ttcgacccct tccacccata ccccacacct ccgtttttcc gcccgccgcg
     1621 gggggcgcgg ccgcgcgcag gctaccaccg ggaccgatgc aatggcaaga ttattaccga
     1681 cttgggcgta cgggcggcca gcggctagcc ttgggtggcc agcgtcagac ggcgggcgtg
     1741 ctcggcagcg aactccgccc aggggtcgtc ggggtactcg gcggggatga tctcgtagca
     1801 gtggagcgcc cgggacaggt ccccgcgctt ggcgtgcaga ttggcagcgc gcaggagcgc
     1861 aaaggccgcc tcggggtggt tcggatggac ctggacgaag ccctcgtacg cgcgaattgc
     1921 gacgtcgggc ttgagatttc gctcgagtcc gaagcacaag tcgagctgcc gctccggttc
     1981 cagcacgaac tcgggcagcg agcgcgaggc ctcctgatag atgcgctccg cttccccgcg
     2041 ccggcccgcc gcgaggagat tctcacacgc actcgcgtag ctcgcgcggg cgccggcgcg
     2101 gtcgccgcac tgggcgcgca cgcgcgccag gagcgcgtgc gcctcgccgt cgctgggacg
     2161 ggcggccgcg tagtgctcga gttcgtcgtt ggcaccccag aagtccccgc gacgcaggca
     2221 tcgacgcgcc ttgagcaagt gcccgtcgac ccgccccttg gcccacccgc cggatgcgat
     2281 ggccacgaac acgccaaagc cgaagcccgc gatgtgcgtc acgtaggcga cgttcaccga
     2341 cgcgccttcg agctggacca gcccgcgcac gagctgaatc aggatccaca gtccgatggc
     2401 cgcgaccgcg tgcagctccg atttgcccgc tttgttggtg gccagcagcg gcgcgaacac
     2461 ccagtacgcg atcttgacgc gatggtgccg caagcgcacg aggaacgcgc ccaacacccc
     2521 ggagacgccg cccgaagcgc cgatgatgcc ggcattggtt tcgagcacgt gtaggttgta
     2581 ccagccctgc agcacgttcc cgaccacggc cgcgcccagg aacacgagcg cgaagccggc
     2641 cggacccagc cgatcctcga ggtagcgccc aaacagcacc aagtagacga ggttggtgac
     2701 gatgtgcagg tagtcgaagt ggaggaaggc ggccgcgagc gcggggccca agttcgagta
     2761 gcccgggaag tagatgtagt tgcgaaactc cagcgcgacc gaaccggcga agaacttgtg
     2821 cagaacgaac acgcccacgc acagcgccgt gcatgcaatc gtgatcgcag gca
//
LOCUS       DASPGK010000380         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_93101, whole genome shotgun sequence.
ACCESSION   DASPGK010000380 DASPGK010000000
VERSION     DASPGK010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93101"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2928
                     /locus_tag="VEC56_10490"
     CDS             <1..>2928
                     /locus_tag="VEC56_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV14618.1"
                     /translation="GLNGSPLGFRADATDDPSTLPLSLAQIIPLDGATDISPTTVLLA
                     TFSRAIEPTTISSESFYLREAPAGPTIAATFGFTDSNRKVSVTPSAALDVATIYEIVL
                     TNAIEASSGGGALTNPSTSDFTTVASVPLNLVSVWPPSAIAGVAVTLSGQGFDPVPSA
                     NTVLFNGVSATPFEGTTDFLRVLVPNTASTGTIRVATSAATSNPVTFTVLIPSTSTID
                     DVIANITTGSSAKSVVISGDGALCYAVGTDGDVVIPVGIEDAFTYPSIPVGDQPVAIV
                     LHPAGALGYVANFNSGTVSVIDVDPDSPDFNTVVGTISVGSNPIDVAVAPDGDRLAVA
                     NAGSNDVSLIDTDETSEAFNEVVATISAGTSAKSVVISGDGTLYVGTSTGVLVIDESN
                     SVVASITAGSSAKSVVISGDGTLLFVLTSDDEILVVDIQDESPSANEVVATIGAGSSA
                     KSVVISADGTLLYIVQEDTDEVLILAIEVIPGVGVTDPDGANSFTIEWSQVGTLPTGD
                     GPADIAVDPSGSGRVIVANAGDFTLTVYGRPFEAVPANFKIVPGVIIPKLPGFYVLGV
                     LQLPPPYTVHDVDIASVRVFGTVPVSPGKYYFGDVNHDGIPDLSMLFCRDEFLAAMPE
                     NGERVDVACTGFVAGEEFEGTDDILVLRPTILTPEENQRIVGGHPFEITWTAPLQVLP
                     CDKVKIEWRHDGDDADDIDCDFHLAEGDETNGLAGASEIDELQRLNESADVNDSGWIV
                     IANHVNNDGDYVWNVPAGYYPNARLRITLLWFGLKVGSSEVPFMIEMTVPTRLKSFDV
                     TMEDGDAVLRWETSLEIGMQGYDIVRSDAEVGRYDVITKEMVRSSGATSGGSYEYRDE
                     TIAANRTYWYKLREVAEDGTGSEYGPYSVTYRLANQLDQNVPNPFNPTTTIRYAIAGD
                     NPVNLTIYDVAGRRVRTLVNERQRADIYKVTWDGMNDAGVRAASGVYFYKLVAGKFTQ
                     TKKM"
BASE COUNT          552 a          944 c          960 g          472 t
ORIGIN      
        1 ggactcaacg ggtccccgct gggcttccgt gccgacgcga ccgacgatcc gagcaccctg
       61 ccgctttcgc tggcgcagat catcccgctc gacggagcga ccgacatcag cccgaccacg
      121 gtgctgctcg cgaccttcag ccgcgccatc gaaccgacga ctatttcgag cgagtccttc
      181 tatttgcgcg aagcgcccgc gggcccgacc atcgcggcca cgttcggatt caccgattcg
      241 aatcgtaagg tctcggtaac cccgtccgcg gcgctcgacg tcgccacgat ctatgagatc
      301 gtgctgacga acgcgatcga agcgagtagc ggcggcgggg cgctgaccaa tcccagcacc
      361 agcgatttca cgaccgtcgc ctcggtgccg ctgaacctcg tttccgtgtg gccgccgagc
      421 gcgatcgcag gcgtcgccgt gacgctttcg gggcagggct tcgaccccgt accctcggcc
      481 aacaccgtgc tgttcaacgg cgtttccgcg actccgttcg aggggaccac ggacttcctg
      541 cgcgtgctcg tccccaacac cgcgtcgacc ggcaccatcc gagtcgccac atccgcggca
      601 acgtcgaacc cagtaacctt tacggtgctc attccgtcga catccaccat cgacgacgtc
      661 atcgccaaca tcacgaccgg gagcagcgcc aagagcgtgg tcatctcggg agacggcgcg
      721 ctgtgctacg ccgtgggcac cgacggcgac gtggtgattc cggtcggcat cgaggacgcg
      781 ttcacgtatc cctcgattcc ggtcggcgac cagccggtcg cgatcgtcct gcacccggcc
      841 ggcgcgctcg gatacgtcgc gaacttcaac tcgggaaccg tctcggtgat cgacgtcgat
      901 cccgactcgc ccgacttcaa caccgtggtc ggaacgatct cggtcgggag caacccgatc
      961 gacgtcgccg tcgctcccga cggcgaccgc ctggccgtgg ccaacgcggg gtcgaacgac
     1021 gtcagcctga tcgacaccga cgagaccagc gaggcgttca acgaggtggt ggccaccatc
     1081 agtgccggaa ccagcgccaa gagcgttgtc atctccggcg acggaacgct gtacgtgggg
     1141 accagcaccg gcgtgctcgt gatcgacgag tccaacagcg tggtggccag catcaccgcc
     1201 ggcagcagcg cgaagagcgt cgtcatctcg ggcgacggga cgttgttgtt cgtccttacc
     1261 agcgatgacg agatcctcgt ggtcgacatc caagacgaga gcccttcggc gaacgaagtg
     1321 gtggcgacta tcggcgccgg ctcgagtgcc aagagcgtcg tcatctccgc ggacgggacg
     1381 ctcctctata tcgtccagga agataccgac gaagttctca tcctggcgat cgaagtcatc
     1441 ccgggcgtgg gtgtgacgga ccccgacgga gcgaacagct tcacgatcga atggagccag
     1501 gtcgggacac tgccgaccgg cgacggcccg gccgacatcg cggtcgaccc gtcggggagc
     1561 ggacgcgtga tcgtcgccaa cgccggcgac ttcaccctga ccgtgtacgg ccgtcccttc
     1621 gaggccgtac ccgcgaactt caagatcgtg ccgggagtca tcatcccgaa gctcccgggc
     1681 ttctacgtgc tgggcgtgct gcaattgccg ccgccgtaca cggtgcacga tgtcgacatc
     1741 gccagcgtac gcgtgttcgg caccgtgccg gtgtcgcccg ggaaatacta cttcggtgac
     1801 gtcaaccacg acggtatccc cgacctctcc atgctgttct gccgtgacga gttcctcgcg
     1861 gccatgccgg agaacggcga gcgcgtggat gtggcgtgca cgggcttcgt cgcgggcgag
     1921 gagttcgagg gcaccgacga catcctggtg ctgcgcccga ccatactcac gcccgaagag
     1981 aatcagcgca tcgtgggcgg acaccccttc gagatcacgt ggaccgcgcc gctccaggtg
     2041 ctcccgtgcg acaaggtgaa gatcgagtgg cgccacgacg gcgacgacgc ggacgacatc
     2101 gactgcgatt ttcatctcgc cgagggcgac gagacgaatg gactcgcggg ggccagcgag
     2161 atcgacgaac tgcagcgctt gaacgagagc gcggacgtga acgacagcgg ttggatcgtg
     2221 atcgcaaacc acgtgaacaa tgatggggat tacgtgtgga acgttccggc gggctactac
     2281 cccaacgcgc gcctgcggat cactctgctg tggttcgggc tcaaggtggg gagcagcgaa
     2341 gtgccgttca tgatcgagat gacggtaccg acgcgtctga agtccttcga cgtgaccatg
     2401 gaggacgggg acgcggtact gcgctgggag acgagcctgg agatcggcat gcagggctac
     2461 gacatcgtgc gctcggacgc cgaggtgggg cgttacgacg tgatcaccaa ggagatggtg
     2521 cgctcgagtg gggcgaccag cggaggaagc tacgagtacc gcgacgagac catcgcggcg
     2581 aaccggacct actggtacaa gctgcgcgag gtggcggagg atgggacggg ctcggagtac
     2641 ggcccgtact cggtgacgta ccgcctggcg aaccagttgg accagaacgt tcccaatccg
     2701 ttcaatccga ccacgacgat acgttacgcg atcgcggggg acaatccggt caacctcacg
     2761 atctacgacg tggcgggtcg gagggtgcgc acgttggtga acgagcgcca gcgtgcggac
     2821 atctacaagg tgacctggga cgggatgaac gacgcgggcg tgagagcggc gagcggggtg
     2881 tacttctaca agctcgtggc cgggaagttc acccagacca agaaaatg
//
LOCUS       DASPGK010000381         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_210358, whole genome shotgun sequence.
ACCESSION   DASPGK010000381 DASPGK010000000
VERSION     DASPGK010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210358"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VEC56_10495"
     CDS             complement(<1..759)
                     /locus_tag="VEC56_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV14619.1"
                     /translation="MQLAALLLLVAVEFGCSTKSVGLTPIRPTHDDKVDSNSVVYTYR
                     IVEDDSLEAYVFRPSSSTDGRPANAILLFHGGGWVAGSPEWTFGAAERFAGYGLVAIA
                     IEYRLSGPNVTPIDALDDVCHAFSWARQYAKDLGIAEKIVGYGVSAGGQLAAATGTIG
                     CGGISADPDALLLWSPAIDVTRDGWFSKLLDGRAEPIDYSPVDHVNASSPPTCIVQGA
                     EDSLTPLSNAQRFCEELTNAGVVCELNVYSGVGHL"
     gene            complement(765..1259)
                     /locus_tag="VEC56_10500"
     CDS             complement(765..1259)
                     /locus_tag="VEC56_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HXV14620.1"
                     /translation="MRTIVSSIESEYRRYKLLAEAAVHQLDDAQLAEDGNGAVNSIAT
                     LVWHLSENIKSRFTDFLTTDGEKPWRDRESEFLPRRVTKAELLSKWEEGWRVLFASLA
                     ALDDGHLARSVTIRTKSLTVPEALHRSLAHVAYHVGQIVFIAKATRGADWEYLSIPPG
                     KSSE"
     gene            complement(1269..1745)
                     /locus_tag="VEC56_10505"
     CDS             complement(1269..1745)
                     /locus_tag="VEC56_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14621.1"
                     /translation="MRHTPHVAFVILTVVFGACAPKAPVDLAAEREALLALHERILDA
                     HRTDNLDDWLALEADTVVVGSRGELFRSPKSESAGMRRNYLAAAEFSAYRDLQPPIVH
                     VSGDGSLGWVFAQVEIVGRYLVPDTTSIHDVWAWVELYERRPEGWRLVGNVSTAKP"
     gene            complement(1865..>2682)
                     /locus_tag="VEC56_10510"
     CDS             complement(1865..>2682)
                     /locus_tag="VEC56_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HXV14622.1"
                     /translation="GYVARTPELWLRDTDSGDVRRVLPPDSVVMDPAPSPDGTRIAFV
                     IADYFQNTGDIFVMDHDGANFQQITFDSELDDQPSWSPDGTRIAFRSWQAGYLGDIWV
                     MDADGGNQVRLTFDPLPGVWDESHPAWSPDGTRIAYAGNAGGNYDIWTMAIDGSDSLQ
                     ITNDEDFETEPAWSPDGSVIVFRRCDNVEGCDLFLIAAGGGAVLPIPRAGEQRMPVFT
                     PDGARIVFVDQETGFHSPDLFSIRPDGTGAAPLVTDELPGGSLNPAFLRRRSR"
BASE COUNT          479 a          895 c          853 g          455 t
ORIGIN      
        1 caggtgcccc acgccggagt acacgttgag ctcgcacacg actcccgcat tcgtcagttc
       61 ctcacagaag cgctgcgcgt tggacagcgg cgtgagcgaa tcctccgcgc cctgcacgat
      121 acacgtcgga ggcgacgacg cgttgacgtg gtcgacgggt gagtaatcga tcggttctgc
      181 gcgcccgtcc agcaacttcg aaaaccatcc atcccgggtg acatcgattg ccggcgacca
      241 tagcaagagc gcgtccggat ccgccgagat cccaccacac ccgatggtac cggtcgccgc
      301 cgccagctgt cctccagcgg acacgccgta gcccacaatc ttctccgcga tgcccagatc
      361 cttcgcgtac tggcgcgccc acgagaacgc gtggcacacg tcgtcgagcg cgtcgatcgg
      421 cgtgacgttg ggacccgaga gccgatactc gatcgcgatc gcaacgagtc cgtagcccgc
      481 gaagcgctcg gcggcgccga aggtccactc gggcgagccc gcgacccaac ctccgccgtg
      541 aaacaagagg atggcattcg ccggtcgtcc atcggtgctg gacgacgggc ggaacacata
      601 cgcctccagc gagtcgtcct ctacgatgcg atacgtgtac acgaccgagt tcgaatcgac
      661 cttatcgtca tgcgtgggac ggattggcgt gagtccgacg cttttcgtcg agcacccgaa
      721 ttccacggcc accaggagca acagcgccgc aagctgcatt tcggctactc cgacgacttc
      781 cccggcggaa tgctcaagta ctcccagtcg gcgccgcgcg tcgccttggc gatgaagacg
      841 atttggccca cgtgataggc gacgtgcgca agcgatcgat ggagcgcctc cggcaccgtc
      901 agcgacttgg tgcggatcgt caccgagcgc gcgaggtggc cgtcgtcgag cgccgccaag
      961 gacgcgaaca gaacgcgcca gccttcctcc catttcgaca gtagctccgc cttggtcacc
     1021 cggcgcggaa ggaactccga ctcgcgatca cgccacggct tctccccgtc ggtagtgagg
     1081 aaatccgtga agcgcgactt gatgttctcc gacaggtgcc ataccagcgt cgcgatagaa
     1141 ttgacggcgc cattgccgtc ctccgccaac tgcgcgtcgt cgagttgatg cacggcggcc
     1201 tccgcgagaa gcttgtagcg gcgatactcg gattcgatcg agctcacgat cgttcgcatg
     1261 gtgctccttt atggcttggc ggtggacaca ttgcccacca aacgccagcc ttccggacga
     1321 cgttcgtaca attcgaccca ggcccacacg tcgtggatcg acgtcgtgtc gggcacgaga
     1381 taccgcccca cgatctcgac ctgcgcgaac acccagccca gcgatccgtc gccggagacg
     1441 tgcacgatgg gtggctgcag gtcgcggtat gccgaaaact cggccgcggc gaggtagttt
     1501 cgccgcatcc cggcgctctc tgacttgggg cttcggaaca actcgccgcg gcttccgacc
     1561 acgacggtgt ccgcttccag cgcgagccag tcgtcgaggt tgtccgtgcg gtgggcgtcg
     1621 aggattcgct cgtgcagcgc gagcagcgct tcgcgctcgg cggcgaggtc gacgggcgct
     1681 ttcggggcgc acgcgccgaa gacgacggtc aggatcacga acgcgacgtg cggcgtgtgc
     1741 ctcatgacac ggagcttacc acacgtgcta gggtcgccgc cctcgtcctg gggcggtatc
     1801 aaagcgatgg atcgttggcc ggcgcgatgc ggtgccggct catctccatc aaagtgccga
     1861 ttatttagcg cgaccgccgc cgcaggaacg ccgggttgag acttccgccc ggcaactcgt
     1921 cggtgacgag cggggccgcg ccggtgccat ccggccgaat gctgaaaagg tcgggcgagt
     1981 gaaaacccgt ctcctggtca acgaagacga tgcgcgctcc gtccggggta aacaccggca
     2041 tgcgctgctc tcccgcgcgc ggtatcggaa gtacggcgcc cccgcccgcg gcgatgagga
     2101 agaggtcgca gccctcgacg ttgtcacagc gacgaaacac gatcacgctc ccgtccggcg
     2161 accacgccgg ctcggtctcg aagtcctcgt cgttcgtgat ctgcagcgaa tcggagccgt
     2221 cgattgccat ggtccagata tcgtagttgc cgcccgcgtt gcccgcgtat gcgatgcgcg
     2281 tcccatcggg tgaccacgcg ggatgactct cgtcccacac gccgggcaac gggtcgaaag
     2341 tgagccggac ttggttgccg ccgtcggcgt ccatcaccca aatgtcgccc aagtagcccg
     2401 cctgccagct tcgaaaggcg atacgggtcc cgtccggcga ccacgagggt tggtcgtcca
     2461 actcggagtc gaaggtgatc tgctggaaat tcgcgccatc gtggtccatg acgaagatgt
     2521 caccagtgtt ttggaagtag tcggcgatca cgaacgcgat gcgggtcccg tcgggcgagg
     2581 gtgcgggatc catcacgacg gagtcgggtg gtagcacgcg ccgcacatcg ccgctgtccg
     2641 tgtcgcgcag ccacagttcc ggcgtgcggg cgacgtagcc ct
//
LOCUS       DASPGK010000382         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_445406, whole genome shotgun sequence.
ACCESSION   DASPGK010000382 DASPGK010000000
VERSION     DASPGK010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445406"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..898
                     /locus_tag="VEC56_10515"
     CDS             131..898
                     /locus_tag="VEC56_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14623.1"
                     /translation="MRRRLGMAGVGAAALLLAACTTGAHAGLDQSIDNARYPTAGIVA
                     HREYRAMGRATPESCALFGGRVGFKDVAHVGVFYGVFNLIDRGDPVFYDHVGFDVRVR
                     LAAESRWPALAAGFDSQGWGPYNGDLKRYERKSVGFYLVLSKNWRSFAGDLSLTAGAN
                     YSLETRDDDRSPDFFAGADWFIARRVSLLVDFDAARNDNAEDGIYGEGGVYVDAGVRV
                     ILGETVSLMLVFSDLTENMAPGGESGRELQLVFADMF"
     gene            934..1914
                     /locus_tag="VEC56_10520"
     CDS             934..1914
                     /locus_tag="VEC56_10520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXV14624.1"
                     /translation="MVQRAPKRHVLDFEQPLIELEDKITELKQLAVVQNVSVDEEVGR
                     LSEKAERLRGEIFAKLTAWQQVQLARHPLRPYTLDYVSALTTDFVELHGDRTFADDAA
                     IVGGFMTINDRRVMMIGHQKGRDTRENIRRNFGMPQPEGYRKANRLMRLAEKFHLPIV
                     TLIDTPGAYPGLGAEERGQALAIAENLKLMAGLRVPIVSVVIGEGGSGGALALGVADR
                     IIMLQHAIYSVISPEGCAAILWKDQTKVKEAADALKLTAKHLLSFGVIDRVVPEPPGG
                     AHRDPATMADTLGQVLYEEVNYLSHVSANVLRERRLKKFLNMGYFEQESP"
     gene            1991..2164
                     /locus_tag="VEC56_10525"
     CDS             1991..2164
                     /locus_tag="VEC56_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXV14625.1"
                     /translation="MVRPELLEILACPKCKEKVVLDERGRYLVCVRCRIRYPVNEHGI
                     PIMLIERAEPLDA"
     gene            2227..>4946
                     /locus_tag="VEC56_10530"
     CDS             2227..>4946
                     /locus_tag="VEC56_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXV14626.1"
                     /translation="MRSAKTVALIITLALTSGRAMAGVDLRTVSDSNGRVVVELTKND
                     GLIGSQPVTFFCAVSPTGTFSARYRIGPAPAPGARAEAGVAIDSVTVSQPFPMRGTRM
                     IWVTVRIPTDTPISNVTIDYTPAVAFNDAAGADPIVRSMVVNRDVFPVAPRAAQSDPW
                     FSRAPGWAKLTVATRGMYAVRGADLAAEGITLGTIDPSTLRVFTSGGLNQSREYTDPT
                     GSWRVGSAMREVPIRVDAGSDGTFDPGDRIYFYGLPSQDWADYYDPAAPDTLYHEHTH
                     AKSTVYFLAWGGDLGGTPARIVDLASAPTADPDLTTYRHREYRERDLLEDYDYRGDGW
                     LWLAMDPPSASRTRLATVDVRNLVTSRPQTFRTVALAKYVEAGAPRGSNFGHHAVYIN
                     YRSGVEFVMGDKIWDGNSGDNFFESGRPVRINGSFLVPGNNEFRLQMPGDLNDEDRIL
                     FAWFSVWYQRRIAAVGNAVGFSSPDTTGVVNFRADGFGGTDAIHLFDVTDPWNPVRVT
                     GLEEAPGTGGRRVRFSSAHAGTGRHFWAATTSGMPVPSIARFTPTDLRAATSAPHLLI
                     VTHRDFRGAADRLRSYRAAGRVPLVNNPTVDIVTSDLIFDNFSQGLPDPMALRNYIKF
                     LYDNYPDANGNPKLAYVCFLGDASTDFRNNVSAQPDFVPSNLYFARLAPFTFATDEWF
                     GHMDPEDQVPGADMLDIAIGRLPAGTPAEADFLVDRVIDYETASPLEKWREEIILVAD
                     DEISSFAGVCETSWTDQSELLTFNHSPDFARITKIYLTEYPVIGTDKPTSRFEFLDEW
                     SDGALMINFIGHGSSQQMADEKVFLGTDVSQLTNGLKLPMMMAFSCTIGDFANPVAKS
                     LSEDLLLHEAGGIIAAVTASRESYPGPNSVLNQSVFDILLPRHLGGSVLPL"
BASE COUNT          812 a         1651 c         1626 g          857 t
ORIGIN      
        1 aaggcgggcg gcgcgcgtac gtgattcgcg cgcgcagcca gggcgtgcgc gtggacaccg
       61 atctgctttc ggggctggcg gcgtcggtcg gagcttccaa cgtgcggctg gtcgcggctg
      121 ggatgggtgc gtgaggcggc ggttgggcat ggcgggcgtg ggcgcggcgg cgctgctcct
      181 ggcggcgtgc acgaccggcg cgcacgccgg gctcgaccag tcgatcgaca acgcgcgcta
      241 tcccaccgcg ggcatcgtcg cgcaccgcga gtatcgcgcc atggggcgcg ccacaccgga
      301 gagctgcgcg ctcttcggcg ggcgcgtggg cttcaaagac gtcgcccacg tgggcgtctt
      361 ctacggggtg ttcaatctca tcgatcgcgg cgaccccgtg ttctacgacc acgtcggttt
      421 cgacgtgcgc gtccgcctgg cggccgagtc gcgctggccc gcgctcgcgg cggggttcga
      481 ctcgcaaggg tgggggccct acaacggcga cctgaaacgc tacgagcgca agtcggtcgg
      541 cttctatctg gtcctgagca agaactggcg ctccttcgcg ggcgacctgt cgctcaccgc
      601 gggtgcgaac tattcgctcg agacgcgcga cgacgaccgc tctcccgatt tcttcgccgg
      661 cgcggattgg ttcatcgcgc ggcgcgtgtc gctcttggtc gacttcgacg ccgcgcgcaa
      721 cgacaacgcc gaggacggga tctacgggga aggcggggtg tacgtcgacg cgggggtgcg
      781 ggtcatactt ggagaaacgg tctccctgat gctagtattc agcgacctga ccgagaacat
      841 ggcccccggc ggcgaatccg gccgcgaatt gcagctcgtg ttcgccgaca tgttctgatg
      901 cggagcgcgc gcggtaagga aggcgaagcc aagatggtgc agcgagcccc caagcgacac
      961 gtcctcgatt tcgagcagcc gctgatcgag ctggaagaca agatcaccga gctcaagcag
     1021 ctcgcggtgg tgcaaaacgt ctccgtcgac gaagaagtcg gtcgcctgtc ggagaaagcc
     1081 gagcgtctgc gcggcgagat cttcgccaag ctcaccgcgt ggcagcaggt ccagctcgcg
     1141 cggcacccgc tgcgccccta caccctcgac tacgtctccg cgctcaccac cgacttcgtc
     1201 gagctgcacg gcgaccgcac cttcgcggac gacgccgcca tcgtgggcgg cttcatgacc
     1261 atcaacgacc gtcgcgtgat gatgatcggc caccagaagg gacgcgacac gcgcgagaac
     1321 attcgccgca acttcggcat gccgcaaccc gagggctatc gcaaggcgaa tcgtctcatg
     1381 cgtctcgcgg agaaatttca cctgcccatc gtgaccttga tcgacacgcc gggcgcgtac
     1441 ccggggctcg gcgccgagga gcgcgggcag gcgcttgcga tcgccgagaa tttgaagctc
     1501 atggcgggct tgcgtgtgcc catcgtgtcg gtggtgatcg gtgaaggggg cagtgggggg
     1561 gcgctggccc tcggggtcgc ggaccgcatc atcatgctcc agcacgcgat ctattccgtc
     1621 atctctccgg aggggtgtgc cgccattctg tggaaggacc agaccaaggt gaaggaggcg
     1681 gccgacgcgc tcaagctgac cgcgaaacac ttgctgtcct tcggtgtgat cgaccgcgtg
     1741 gttccggagc cgcccggtgg ggcccaccgc gacccggcca cgatggcgga tacgctgggc
     1801 caggtcctgt atgaggaagt caattatttg tctcacgttt ccgcgaatgt cttgcgcgag
     1861 cgcaggctca agaaattcct caacatgggc tacttcgagc aagaatcgcc ttgaccgtcg
     1921 ccgcgcgcgc ggagtaacat ctttttcaga ttgaagttat cccgccccta gttccgtcga
     1981 ggtgtcccct atggtccgac ccgagctcct ggagattctg gcgtgcccga agtgcaagga
     2041 gaaggtcgtg ttggacgaaa ggggacggta tctcgtgtgc gtgagatgcc ggatccgtta
     2101 tccggtcaac gagcacggga ttcccatcat gctcatcgaa cgggcggaac ccctggacgc
     2161 gtagaggagc ccttgagcaa cgggcggcgc gcgatttacg gcggccgcca ccatcgtccc
     2221 ggcagcatgc gttcagcgaa aaccgtcgcg ctcattatca cgcttgcgct cacgtcgggc
     2281 cgggccatgg ccggcgtcga tctgcgaacc gtttccgaca gcaacgggcg cgtcgtggtc
     2341 gaattgacca agaacgacgg cctcatcggc tcccagcccg tcaccttctt ctgcgccgtc
     2401 tcgccgaccg gaaccttcag cgcgcggtat cgcatcggtc ctgcgcctgc gccgggcgcc
     2461 cgcgccgagg ccggtgtggc catcgactcg gtcacggtgt cgcagccgtt tcccatgcgc
     2521 ggcacgcgca tgatctgggt caccgtccgc attcccaccg acacgccgat ttcgaacgtc
     2581 actatcgact acacgccggc cgtcgccttc aacgacgcgg ccggcgccga cccgatcgtg
     2641 cgcagcatgg tggtcaatcg cgacgtgttt ccggtggcgc cgcgtgcggc gcagagtgac
     2701 ccctggttct cgcgcgcgcc cggctgggcc aagctcacgg tggcgacgcg cggcatgtac
     2761 gccgtcaggg gcgccgacct cgctgcggag ggcatcacgc tggggaccat cgatccgagc
     2821 acgctgcgcg tgttcacgag cgggggcctc aatcagtcgc gtgagtacac cgacccgacc
     2881 gggtcgtggc gcgtgggatc cgcgatgcgc gaggttccga ttcgcgtcga tgccgggagc
     2941 gacgggacct tcgacccggg tgaccgcatc tatttttacg gcctccccag ccaagattgg
     3001 gcggactatt acgaccccgc cgcgcccgac acgctctacc acgagcatac ccacgcgaag
     3061 tcgacggtct atttcctggc ctggggaggc gacttgggtg gcacgccggc gcgtatcgtc
     3121 gatctcgcgt ccgcccccac cgcggatccg gacctcacca cgtatcgtca tcgcgagtac
     3181 cgcgagcgcg atctcttgga ggactacgac tatcgcggtg acgggtggtt gtggctggcg
     3241 atggatccgc ccagcgcctc gcgcacccgg ctagcgaccg tcgacgtgcg caacctggtg
     3301 acgtcgcggc cgcagacgtt ccgcaccgtg gcgctggcca agtacgtgga ggcgggcgcg
     3361 ccgcgcggga gcaacttcgg acaccacgcc gtctatatca attatcgcag cggcgtcgag
     3421 ttcgtgatgg gggacaagat ctgggacggt aactcgggcg acaacttctt cgagtcgggg
     3481 cgtccggtgc gtatcaacgg cagcttcctc gtacccggta acaacgagtt tcgcttgcag
     3541 atgccgggcg acttgaacga cgaggatcgc attctgtttg cgtggttctc ggtgtggtac
     3601 caacggcgca tcgcggcggt cggcaacgcc gtgggcttct cctcgcccga caccaccggc
     3661 gtggtgaact tccgcgccga cgggttcggc gggacggatg ccatccacct gttcgacgtg
     3721 accgacccgt ggaatcccgt gcgcgtaact ggcctcgaag aggcgccggg tacgggcggg
     3781 cggcgcgtgc gcttttcctc cgcccacgcg ggcaccgggc gtcatttctg ggcggcgacc
     3841 acgagcggca tgccggtgcc gtccattgcg cgcttcaccc ccacggattt gcgcgccgct
     3901 accagcgccc cgcacctctt gatcgtcacc caccgcgact ttcgcggcgc cgccgaccgc
     3961 ttgcgcagct accgggccgc cgggcgcgta ccgctggtga acaacccgac cgtggacatc
     4021 gtgacctccg acctcatctt cgacaatttt tcccagggac tgccggaccc gatggcgttg
     4081 cgcaattaca tcaagttcct gtacgacaac taccccgacg ccaacggcaa cccgaagctc
     4141 gcgtacgtgt gcttcctggg cgacgccagc accgacttcc gcaacaacgt cagcgcgcaa
     4201 cccgacttcg tgccgtccaa cctgtacttc gcgcggctgg cgccgttcac gttcgccacc
     4261 gacgagtggt tcgggcacat ggacccggag gaccaggtac ccggcgccga catgctcgac
     4321 atcgcgatcg ggcgtttgcc cgcgggcacg ccggcggaag ccgatttcct ggtcgaccgc
     4381 gtgatcgatt acgagaccgc ctcgcccctc gagaagtggc gcgaggagat cattctggtc
     4441 gccgacgacg agatctcgtc gttcgcgggg gtgtgcgaga cgtcgtggac cgaccagtcg
     4501 gagttactca cgttcaacca ctctcccgac ttcgcgcgca tcacgaagat ctacctcacc
     4561 gaatacccgg tgatcggaac cgacaagccg acgtcgcgct tcgagttcct ggacgagtgg
     4621 agcgacggcg cgctcatgat caacttcatc gggcacggaa gctcgcagca gatggcggac
     4681 gagaaggtct ttctcgggac cgacgtgagc cagctgacca acggcctcaa gctcccgatg
     4741 atgatggcgt tcagctgcac catcggcgac ttcgcgaacc cggtggccaa gagcctctcg
     4801 gaggacctct tgctccacga agcgggcggc atcatcgcgg ccgtgaccgc ctcgcgcgag
     4861 tcgtatcccg gtcccaacag cgtcttgaac cagtcggtgt tcgacatcct gctgccgcgc
     4921 cacctgggcg ggagcgtgct cccgct
//
LOCUS       DASPGK010000383         6489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1264372, whole genome shotgun sequence.
ACCESSION   DASPGK010000383 DASPGK010000000
VERSION     DASPGK010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264372"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VEC56_10535"
     CDS             <1..313
                     /locus_tag="VEC56_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14627.1"
                     /translation="SKYASRYHADAPAPALLTISLNPFAPPGYVPPTRFDAMLFGAGT
                     AGTLAMFVGAIGNTLGAFDEDTTWIITGAAAAAGALYSGSRFKVRLAPDDHSPRPPSD
                     R"
     gene            complement(371..3646)
                     /locus_tag="VEC56_10540"
     CDS             complement(371..3646)
                     /locus_tag="VEC56_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV14628.1"
                     /translation="MFYRMLIAACALLFASSHTATAQDAKKKDDKKKWDVEAPLGATN
                     AVEFTTNEGTWMNLDVSPDGKEIAFDLLGDIYIMPIAGGKARLLRGGPAFEVQPRFSP
                     DGTRISYTSDKDGADNVWVMKRDGSDPKQVTKEDFRLVNNAVWTPDGQFLVAKKHFTS
                     RRSLGAGEMWLYHISGGEGLQVTKRRNDQQDVGEPCVSRDGRYVYFSEDMSPSPVFQY
                     NKDPNGEIYAIRRADRETGEVKDVVTGPGGGVRPQVSPDGKSLAYVHRVRAKTVLYVQ
                     DLESGAQRPVWDGLSKDQMETWATFGVYPNFQWTPDNKSIVLWAKGKIYKVDLATRAA
                     TNIPFEVDAKHTINAALRFPQDPAPATFDAKMIRHAATSPDGSWLVFSAVGHLWKKKL
                     PDGVPARVTKDTHWEYWPSFSRDGKWLVYTTWDNDAAGSVYKVKLDGGGKKKLTTRPG
                     FYHAPSFSPDGKRVVFQRGTGNVILGVSYGTEPGLYWISADGGEATLIVEDGEMPRFS
                     KDGERVYFVADAPEQKQAYKSVRVDGGDERVLFSSKYATQFVPSPDEQWLAFTELFQA
                     YIVPFAKTGKEVDLNAKTKAVPIKQVTRDAGSFLHWSGDSKKLHWTIGPEYFTREIAK
                     SFDFVEGAADSIAPPDTSGAAIGLKLPSDVPTGVVAFTNARIITMKGDEVIENGTVVV
                     DKNRILAVGPASSVEVPRGAHLVECGGKTIMPGLIDVHAHMWHPSITPSQSWLYLANL
                     AFGVTTTHDPSNPTETVFTNSEMVKAGELVGPRVFSTGTILYGADGDFKAVINSLDDA
                     RSHLRRMKAVGAFSVKSYNQPRRNQRQQVIKAARELEMMVVPEGGSFFYHNLSMILDG
                     HTGVEHTIPIAPIYKDVTSLWGASKTGSTPTLIVGYGGLWGEEYWYQKTNVWENGRLL
                     TFTPRAVIDERSRRRTMSPDDEFNHIALAKNCKDLTDAGVSVQLGAHGQLQGLGAHWE
                     LWMFVQGGMSNLQALRAATLNGAWYIGMDKDLGSLEAGKLADLIVLDKNPLEDIRSSE
                     SIVYTMVNGRLFDARTMDQIGNHPEKRLAFYWEKPGASEAFVWQGEATGYMPGGCGCF
                     GTH"
     gene            complement(3691..4500)
                     /gene="proC"
                     /locus_tag="VEC56_10545"
     CDS             complement(3691..4500)
                     /gene="proC"
                     /locus_tag="VEC56_10545"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXV14629.1"
                     /translation="MLRIAILGAGNIGTSIAEGLVQSGRFSAPGITLTRRTVQRVEAM
                     KQKGFVVHADNTRAVKESEIVIVAVEPQQVDAVLGEIAPSVTDRHVLISVVTGVSIAQ
                     IEGKVGKRAAVVRAMPNTAIAVRESMTCLASRDPASAAVATATSVFDAVGKTVLINEE
                     EMIAATALGACGVAFFLRAIRAASQGGIEVGLHSHSALAMAAQTAKGAATLLLAMGTH
                     PEQEIDRVTTPKGCTIAGLNQMEHAGFSSAMIRGITVSADKASRLYADDDA"
     gene            complement(4520..4978)
                     /locus_tag="VEC56_10550"
     CDS             complement(4520..4978)
                     /locus_tag="VEC56_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV14630.1"
                     /translation="MAGEIDNLIDRANIVDVINTLFVGTDNRNWDVVRNCFAPSVLFD
                     MSSLGAGEPHHMTPNEIVAAWDAGLKPMQAIHHQVGNYLVTVDGPKSASAFCYGIAIH
                     YLPNPTHANTRTFVGSYDFGLTKENGRWKIEKFKFTLKYVDGNLKLETSI"
     gene            complement(5078..5647)
                     /locus_tag="VEC56_10555"
     CDS             complement(5078..5647)
                     /locus_tag="VEC56_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14631.1"
                     /translation="MTDRPHSGDDIAAECRRIEIHIEELSQLFDSMDPSPFHKKDLDR
                     NAAEYIASSALDLPRRVPLALVVYLDKPPELTDEGRVVGDAVREHFARESLYARRRLH
                     QLLRRGWVSLAIGVTFLATAFVAGTFVSRMVAETLRPFVRESLLIGGWVAMWRPLETF
                     LYDWWPIVGERRRYDRLSRMAVRIEYKGT"
     gene            complement(5698..>6489)
                     /locus_tag="VEC56_10560"
     CDS             complement(5698..>6489)
                     /locus_tag="VEC56_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013051896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXV14632.1"
                     /translation="RNGAWMSEYRQQSRVGGKEVTPIIVNVSNVSRATAEEPALLSLD
                     EVATLFHEFGHALHGMLSDVTYSSLAGTNVARDFVEMPSQVIENWATQPEVLKMYARH
                     YQTGATIPDDLIAKMTNAKLFNQGFVTVEYLAASYLDMDWHTMTGEAPKDVVAFEKAR
                     MDQIGLIPEIVPRYRTTYFRHIFAGGYSAGYYSYIWAEVVDADAFQAFKEKGLFDQET
                     AAKFRKYILSAGGTDDPMALYKKFRGREPEIGPLLERRGLTTTDS"
BASE COUNT         1027 a         2148 c         2095 g         1219 t
ORIGIN      
        1 atcgaagtac gcatctcgct accacgcgga tgccccggct cccgcgctcc tcacgatatc
       61 gctcaatccg ttcgcgcccc caggatacgt cccgccgacc cgcttcgacg cgatgctctt
      121 cggcgcggga acggccggaa cgctggccat gttcgtggga gcgatcggca acacgctggg
      181 cgccttcgac gaagacacga cgtggatcat aaccggcgcc gccgccgccg caggggcgtt
      241 gtacagcggc tcgcgcttca aggtgcgcct cgctcccgac gaccactcgc cgcgaccgcc
      301 ttccgaccgc tagcccgccc acacgatgat gcgggcggga cgcgcgcgcc accgcagagt
      361 gaacgtcgaa tcagtgcgtg ccgaagcagc cgcacccacc cggcatgtac cccgtcgctt
      421 cgccctgcca cacgaaggcc tcgctcgcgc ccggcttctc ccagtagaac gcgagtcgct
      481 tctcgggatg gttgccgatc tggtccatgg tacgcgcgtc gaacaaccgg ccgttcacca
      541 tggtgtagac gatgctctcg ctgctgcgta tgtcctcgag cgggttcttg tcgagcacga
      601 tcaggtcggc cagcttgccc gcttccaagg aacccaggtc cttgtccatg ccgatgtacc
      661 aggcaccgtt caaggtcgcc gcgcgcaacg cctgcaagtt cgacattccg ccctgcacga
      721 acatccacag ctcccagtgc gcgccgagcc cctggagctg gccgtgcgcg cccaactgca
      781 cgctgacgcc ggcatcggtc aggtccttgc agttcttcgc cagtgctatg tggttgaact
      841 cgtcgtcggg cgacatggtg cggcgccggg accgttcgtc gatcaccgcg cggggggtga
      901 acgtcaatag gcgcccgttc tcccatacgt tggtcttctg ataccagtac tcctcgcccc
      961 acaggccgcc gtagccgaca atcagcgtgg gcgtcgaacc ggttttgctc gcaccccaaa
     1021 gactcgtcac atccttgtaa ataggcgcga tggggatggt gtgctccacg ccggtgtggc
     1081 cgtcgagaat catcgagaga ttgtgataga agaacgagcc gccttccggc accaccatca
     1141 tctcgagctc gcgcgcggcc ttgatcacct gctggcgctg gttacggcgc ggctggttgt
     1201 aactcttgac cgaaaacgcg cccaccgcct tcatgcgccg caggtgcgag cgcgcgtcgt
     1261 cgagcgagtt gatcacggcc ttgaagtcgc cgtcggcgcc gtacaggatg gtgccggtcg
     1321 agaacacgcg cggtcccacc agctcgcccg ccttcaccat ctccgagttg gtgaacacgg
     1381 tctcggtcgg gttcgacggg tcgtgggtcg tggtgacgcc gaaggccagg ttggcgaggt
     1441 agagccaact ctgcgacggg gtgatcgacg ggtgccacat gtgcgcgtgg acgtcgatca
     1501 acccgggcat gatggtcttg ccgccgcact cgacgaggtg cgcgccgcgc ggcacttcca
     1561 ccgacgacgc cggccccacc gccaggatgc ggttcttgtc caccaccacg gtaccgtttt
     1621 cgatcacctc gtcgcctttc atcgtgatga tgcgtgcgtt ggtgaaggca acgacaccgg
     1681 tgggcacgtc gctggggagc ttcaggccga tggccgcgcc gctggtgtcg ggcggggcga
     1741 tcgaatccgc ggcgccctcc acgaagtcga agctcttcgc gatctcgcgc gtgaagtact
     1801 cggggccgat ggtccagtgc agcttcttgc tgtcgcccga ccagtgtaaa aagctgcccg
     1861 cgtcgcgcgt gacctgcttg atcgggaccg ccttggtctt ggcgttgagg tcgacttcct
     1921 tgcccgtctt cgcgaacgga acgatgtagg cctggaacaa ctcggtgaac gcgagccact
     1981 gctcgtcggg gctggggacg aactgggtcg cgtacttgga cgagaacagg acgcgctcgt
     2041 cgccgccgtc cacgcgcacg ctcttgtagg cctgcttctg ctcgggcgcg tcggcgacga
     2101 agtagacgcg ctcgccgtcc ttgctgaagc gcggcatctc gccgtcttcg acgatcaacg
     2161 tcgcctcgcc gccgtccgcg gaaatccagt acagcccggg ttcggtgccg tacgacacgc
     2221 ccaggatcac gttgccggtc ccgcgttgga acaccacccg cttgccgtcg ggcgaaaacg
     2281 aaggcgcgtg gtagaagccg gggcgcgtcg tcagcttctt cttgccgccg ccgtcgagct
     2341 tgaccttgta gaccgacccg gccgcgtcgt tgtcccaggt cgtgtacacg agccatttcc
     2401 cgtcgcgcga gaacgacggc cagtactccc agtgcgtatc cttggtgacg cgggcgggaa
     2461 cgccgtcggg gagcttcttc ttccagaggt gcccgactgc gctgaacacg agccaacttc
     2521 cgtcgggcga ggtggcggca tgacggatca tcttggcatc gaaggtggcc ggcgcgggat
     2581 cctgcgggaa tcgcagcgcg gcgttgatcg tgtgcttcgc gtcgacctcg aaggggatgt
     2641 tggtcgcagc gcgcgtggcc agatccacct tgtagatctt gcccttcgcc cacaggacga
     2701 tggacttgtt gtcgggcgtc cattggaagt tgggatacac gccgaaggtg gcccacgtct
     2761 ccatctgatc cttgctcaac ccgtcccaga ccggtcgctg cgcgccgctc tccagatcct
     2821 gcacgtacag cacggtcttc gcgcgcacgc ggtgcacgta ggcgaggctc tttccgtccg
     2881 gcgagacctg cgggcgcacg cccccgccgg gtcccgtgac gacgtccttc acctcgccgg
     2941 tctcgcggtc ggcgcggcgg atcgcgtaga tctcgccgtt gggatccttg ttgtactgga
     3001 agaccgggct ggggctcatg tcctcgctga agtaaacgta acgcccgtcg cgcgagacgc
     3061 agggttcgcc gacgtcctgc tggtcgttgc ggcgcttggt aacctgcagc ccttcgccac
     3121 cgctgatgtg atagagccac atctcgcccg cgcccagcga gcgacggctc gtgaagtgct
     3181 tcttcgccac caggaactgg ccgtcgggcg tccacacggc gttattgacc aggcgaaaat
     3241 cttccttggt gacctgcttg ggatcggagc cgtcccgctt catgacccaa acattgtccg
     3301 cgccgtcctt gtcggaggtg tagctgatgc gcgtgccgtc gggcgagaag cgcggctgca
     3361 cttcgaaggc cggcccgccg cgcaacagcc tcgccttccc gcccgcgatc ggcatgatgt
     3421 agatgtctcc caacagatcg aacgcgatct ccttcccgtc cggggagacg tcgagattca
     3481 tccacgtgcc ttcgttggtg gtgaactcga ccgcgttggt cgcaccgagg ggagcttcga
     3541 cgtcccactt cttcttgtcg tctttctttt tcgcgtcttg ggcggtagcg gtgtgcgacg
     3601 aagcgaacag caacgcgcac gctgcgatca gcattcggta gaacatgagt gctccttgag
     3661 gttggcttgg aaagaggtcc tgcccattat tcacgcgtcg tcgtccgcgt acaagcgaga
     3721 cgccttgtcc gcggacacgg tgatgccccg gatcatggcc gagctgaacc cggcgtgttc
     3781 catctggttc aagcccgcga tggtgcagcc cttgggcgtg gtgacgcggt cgatctcctg
     3841 ctcggggtgg gttcccatcg ccagcagcag ggtcgctgcg cccttcgcgg tttgggccgc
     3901 catcgccagc gcgctgtgcg agtggaggcc gacctcgatg ccgccctgcg aggccgcgcg
     3961 gatcgcgcgc aggaagaacg cgaccccgca tgcccccagt gcggtggccg cgatcatctc
     4021 ttcctcgttg atgaggacgg tctttccgac cgcgtcgaag acggaagtcg cggtcgcgac
     4081 cgcggcactc gccgggtcgc gcgaggcgag gcaggtcatc gattcgcgca ccgcgatcgc
     4141 ggtgttgggc atcgcgcgca cgaccgcggc ccgcttcccg accttgcctt cgatctgggc
     4201 gatgctgacg ccggtgacga ccgaaatgag gacgtgccgg tcggtgacgg agggagcgat
     4261 ctcgcccagc acggcgtcca cctgctgcgg ttccaccgcg acgatgacga tctccgactc
     4321 cttgaccgcg cgcgtgttgt ccgcgtggac gacgaagccc ttctgtttca tcgcttcgac
     4381 gcgctgcacc gttcgccgtg tcagcgtgat gcccggcgcg gagaaccgac ccgactgaac
     4441 cagtccctcg gcgatggagg tgccgatgtt cccggccccc aggatcgcga tacgaagcat
     4501 tgcctctcct tgttgaatac tagatggagg tctcgagctt gaggttcccg tcgacgtatt
     4561 tgagcgtgaa cttgaacttc tcgatcttcc agcgcccgtt ctctttcgtc agtccgaagt
     4621 cgtagctccc gacgaaggtc cgcgtgttcg cgtgcgtcgg gttgggcaga tagtggatgg
     4681 cgatgccgta acagaatgcg cttgcgctct tcggcccgtc caccgtcacc aggtagttgc
     4741 ccacttggtg gtgaatggcc tgcatcggct tcaacccggc gtcccacgcg gccacgatct
     4801 cgttcggcgt catgtggtgg ggctcgcccg cgcccagcga ggacatgtcg aacaggacgc
     4861 tcggcgcgaa gcagttgcgg accacgtccc agttgcgatt gtcggtgccg acgaacaacg
     4921 tgttgatcac gtcgacgatg ttcgcccggt cgatgaggtt atcgatttct ccagccatca
     4981 accctttcct cggcaccccg cgcgtcgcgg tcccctcccg gcccgcgacg gcccgatcat
     5041 aagcgaaccc gcctcccgac gaaagacccg gaccgggtca ggttcctttg tactcgatgc
     5101 ggaccgccat gcggctcaag cggtcgtagc ggcggcgctc gccgacgatc ggccaccaat
     5161 cgtagagaaa cgtctcgagc gggcgccaca tggcgaccca gccgccgatc aacaggctct
     5221 cgcgcacgaa cggacgcagc gtctccgcga ccatgcggga gacgaaggtg cccgccacga
     5281 aggccgtggc gaggaaggtg acgccgatgg ccaggctcac ccatccgcgg cgcaagagct
     5341 gatgcagccg gcggcgggca tacagcgact cgcgcgcgaa gtgctcgcgc accgcgtcgc
     5401 cgacgacgcg accttcatcg gtgagttccg ggggcttgtc gagatagacc accagggcca
     5461 gggggactcg acgcgggaga tccagcgcgc tgctcgcgat gtactcggcg gcgttgcgat
     5521 cgaggtcctt cttgtggaac ggcgacgggt ccatcgagtc gaagagctga ctcagctcct
     5581 cgatgtggat ctcgatgcgg cggcactcgg ccgcgatgtc gtcgccggaa tggggtcggt
     5641 cggtcatcgg cgctacctta gcacaggccg atcgaccccg tcccggcgac cccgtggtta
     5701 cgagtccgtg gtggtcaagc cgcggcgttc gagcagcgga ccgatctccg gctcgcggcc
     5761 gcggaatttc ttgtagagcg ccatcgggtc atcggtgccg ccggccgaca agatgtactt
     5821 gcggaacttg gccgcggtct cctggtcgaa cagccccttc tctttgaaag cctggaaggc
     5881 gtccgcgtcc accacttcgg cccagatgta gctgtagtac cccgccgagt agccgcccgc
     5941 gaagatgtgg cggaagtacg tggtccggta gcgcggcacg atctccggga tcaacccgat
     6001 ctgatccatg cgcgccttct cgaaggcgac cacgtccttg ggcgcttcac cggtcatggt
     6061 gtgccagtcc atgtcgaggt acgacgcggc caggtactcg acggtcacga agccctggtt
     6121 gaacagcttc gcgttggtca tcttggcgat gaggtcgtcg gggatggtgg cgcccgtctg
     6181 gtagtggcgc gcgtacatct tcaggacctc gggctgggtg gcccagttct cgatcacctg
     6241 cgagggcatc tcgacgaagt cgcgcgcgac gttggtcccc gcgagactcg agtaggtgac
     6301 gtcggagagc ataccgtgca gcgcgtggcc gaactcgtgg aacagtgtgg cgacttcgtc
     6361 gagtgacaac aaggccggct cttcggccgt ggcacgcgag acgttcgaga cgttaacgat
     6421 gatgggcgtg acttccttgc cgcccacgcg cgactgctgg cggtactcgc tcatccacgc
     6481 gccgttgcg
//
LOCUS       DASPGK010000384         6976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_211287, whole genome shotgun sequence.
ACCESSION   DASPGK010000384 DASPGK010000000
VERSION     DASPGK010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211287"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEC56_10565"
     CDS             <1..259
                     /locus_tag="VEC56_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14633.1"
                     /translation="GGMQSTPMDLYRWNQGIRTGKTLSASAAQKYWHGGILAGGDDRG
                     FFTLYNEGPGDMFIMCSNAHNRPGDHASTVGRRLAEMAMGR"
     gene            368..3274
                     /locus_tag="VEC56_10570"
     CDS             368..3274
                     /locus_tag="VEC56_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV14634.1"
                     /translation="MTGQRIAHYEILGRLGSGGMGDVYRARDTRLKREVALKVLPAAI
                     SDDPMRRRRFEREAQVASSIKHPGIVTIYEFGEHEGRSFIAMELVEGDSLRELIGKGP
                     LPIDRALDIAIQVGEGLARAHESGIVHRDIKPENVIIDRDSRARVLDFGLAMPKGQTR
                     LTADGTTVGTTHYMSPEQSTETEVDRRTDIWSLGIVLYEMIVGAVPFQADHEPATIYS
                     ILNEPHQPLTERRTNVPVELERVVDKMLAKNRAERYPSVEDLLVDLRRLRGNSTTMGS
                     ALTHRKSKRTRTMAVVAVAAVAVVAVGIWIGRGDPPVAKSIAVLPFVNMSDDPGNEYF
                     SDGLSEELLNALARLGDLRVAARTSSWRFKGRNDDVADVGRELNVATVLEGSVRRSGE
                     RLRITVQLVNTGDGFHMWSETYDRELGDVFAIQEEIANEVVRELKVRLLQEESAQLAR
                     RPTDNLDAYEAYLRGKHEMARRTSAALQSAADHFKRAVALDPEFALAYVGLADAYRLH
                     GHYGVASYDDFVPEIERLINKAIEIDDRLGEAYASLAVLRGIQDRWAEVAPLFERAIE
                     LAPSYATAYHWYGGSLSDSDTDPAKAGAMLRKAVELDPLSPVINLAYSEHLQQIGQLD
                     EALGRIERALELDDSFPGAYASLANLYYREYGRIDEALGSIDRAIALDPGNIGLLYGR
                     ADYLAGAGRVDEAIAACRAAVDAHPDFAWGYKMAGDVHRKLGRLDEALSFYRRAAEIE
                     REDFWGEFYCALTLFSMGDEEAVEAYIGRMPPIQSGIIEVVYRLCRVELGRANENLEE
                     LVALAGSDFLNDTVADVALAEGRYADAMGTYEQAFPELLRDSDVRVHSGNLDPAIRIA
                     AARIGIGETERARWLIGKCATYLESLPETERHYEFGRQLAEVYALQGRKREALDTLEG
                     RVDAGLRDEWCTQMRSPMLVSIRDDPEFVAVFDEVKNDVAGMRARLTATDRTPP"
     gene            3323..4357
                     /locus_tag="VEC56_10575"
     CDS             3323..4357
                     /locus_tag="VEC56_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14635.1"
                     /translation="MSTRSSLRLGMAVLLALLASLDVRAQEAIEVRDSVIAPVQGISH
                     AIESTVAFFIDEKDLLPESVAYDPKDKSFYVGSTRKGKIVRVDKDGNASDFIASRQDG
                     LWQVIGIKINPTRRILWACSFDGESLEGFKPGDTRATGVFAFNLDTGTLVRKWTIEKP
                     GEVHAFNDLVVTRGNDAYVTHMFDDAAIYKITDRTQSLEVLARPAGLKDPNGITITPN
                     EATLFVAGADGIYAVDRATGASRPLQAAEGDPLGAIDGLYFYRGSLIAVHMTSVRRHR
                     LDNANTRVVSTEILEADHPLFDVPTTGVIVGDDFYYVANSQFSAVQKDGTLLPMDQLN
                     EPAILKLRLR"
     gene            complement(4363..4875)
                     /locus_tag="VEC56_10580"
     CDS             complement(4363..4875)
                     /locus_tag="VEC56_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14636.1"
                     /translation="MSARVAVVATLLCAFAGSAHAQAVISRFVVGSGGTQAASTSYAL
                     VGTAGQPAVGGATGASFQVVGGFWASTNPIGTGVETPRARVFRLEQNYPNPFNPRTTI
                     AFAIPSDTHVRLHVFDATGVRVMTVIDRSLPAGAHRVELDASGLASGVYFYRLEAPGF
                     QQARKLVLLK"
     gene            complement(4872..5708)
                     /locus_tag="VEC56_10585"
     CDS             complement(4872..5708)
                     /locus_tag="VEC56_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14637.1"
                     /translation="MRIVLTLAWVLLAFVPAARADVPGTISYQGLLTDAVGTPVPDDD
                     YSIRFRLYDPALVWEETQTVTVTSGRFSVLLGSVVPIDPAILQGAPLSLGIKVGDDSE
                     LSPWTPLTSVPYALTAAHATTAESVTMSPTTRYHTVCAEAFTPNSSATAYTKNSVRVY
                     PTAGTNISMGAVLTLPHGAQLTELHVLFEDAIPDLELSVGLRRVNVTDGTLQTLGVVA
                     SGIPEAAGLTIATRNLFLPSDIVDNQNWAYSLQAFWSTAAGLNLSLRAVRITYTVTEP
                     LP"
     gene            complement(5870..>6976)
                     /locus_tag="VEC56_10590"
     CDS             complement(5870..>6976)
                     /locus_tag="VEC56_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXV14638.1"
                     /translation="INAAVGGGGGCSPLVTANFSGTPTSGTAPLTVNFTDLSTGSPTS
                     WSWNFGDGGTSTAQNPSHAYAAAGTYTVTLTATNACGSDAEVKSAYVSVTSGGVVWTT
                     ITFDQFETNFGSYTDGGVDCSRYTGGTFSYQGVASIDIQDNSGTPSSFSTTTARNLTA
                     YQTQQITFFFRAQSMETGDNFYVEYYNGSAYQIVANYVAGTSFANGSFYTATITLNRG
                     TYTFPTNARIRFRCDAGDNNDDVYVDEITWRATPTVGASSEIAEPVLVREGDRPIDIT
                     SEAVSFETTLEQNYPNPFNPRTTIAFALASESDVTLEVFDTAGRLVARLLDESKGAGR
                     HTVDFDASRLSSGVYFYRLRAGDFVQQRKMVLLK"
BASE COUNT         1247 a         2224 c         2332 g         1173 t
ORIGIN      
        1 cggcggcatg caatcgacgc cgatggacct gtatcgctgg aatcaaggca ttcgcaccgg
       61 caagacgctc tcggccagcg cggcccagaa gtactggcac ggcggcatcc tggcgggcgg
      121 cgacgaccgc ggcttcttca ccctctacaa cgaagggccg ggcgacatgt tcatcatgtg
      181 ctcgaacgca cacaaccgcc ccggcgatca cgcgtcgacg gtggggcgcc ggctggcgga
      241 gatggcgatg gggcgctgag tcgccgacgc gtcccggcaa cgcgatcctt cgcaaccacc
      301 cgttcccgat gatatcctct cgggcgatga cccgcccagc ccgaccagcc ggcgaggagg
      361 gggccgcatg acgggccagc gaatcgcgca ctacgagatc ctcggaagac tcggcagcgg
      421 aggcatgggc gacgtgtatc gcgcgcgcga cacccgcctc aagcgagagg tcgcactcaa
      481 ggtcctcccc gccgcaatca gtgacgatcc catgcgccga cgccgcttcg agcgcgaggc
      541 tcaggtcgcg tcgtcgatca agcaccccgg catcgtcacc atctacgagt tcggcgagca
      601 cgaaggccgt tccttcattg ccatggagct cgtcgagggc gactcgctgc gcgaattgat
      661 cggcaagggg ccgctgccga tcgatcgcgc gctggacatc gcgatccaag tgggcgaagg
      721 cctcgcaaga gcgcacgaga gcgggatcgt ccatcgcgac atcaagcccg agaacgtcat
      781 catcgatcgg gacagccgcg ctcgcgtcct cgacttcggc ctcgccatgc ccaaggggca
      841 aacccgcctg accgccgacg ggacgacggt cggcaccacg cattacatgt cgcccgagca
      901 gtcgaccgag accgaggtcg atcgccgcac cgacatctgg tcgctgggca tcgtgctcta
      961 cgagatgatc gtgggagcgg tgccgtttca ggccgaccac gaacccgcca cgatttactc
     1021 gatcctcaat gagccgcacc agccgctcac cgaacggcgc accaacgttc ccgtggagtt
     1081 ggaacgagtg gtggacaaga tgctggcgaa gaatcgcgcg gagcgctacc cgagcgtgga
     1141 agacctgctc gtcgacctgc ggcgcctgcg cgggaattcg acgaccatgg gaagcgcact
     1201 cacgcaccgc aagagcaaga ggacgcgaac catggcggtg gttgcggtcg cggcggtcgc
     1261 ggtggtcgcg gtgggcattt ggatcggccg tggtgaccct ccggtcgcga agtcgatcgc
     1321 cgtgcttccg ttcgtgaaca tgagcgacga tcccggcaac gagtacttct cggacggact
     1381 ctcggaagaa ctgctgaacg cgttggcgcg cctgggcgac ctgcgcgtgg ccgcgcgaac
     1441 gtcgtcgtgg cggttcaagg gacgcaacga cgacgtggcc gacgtcggtc gcgagctcaa
     1501 cgtggcgacc gtgctcgagg gcagcgtacg gcggtccggc gaacgccttc gcatcacggt
     1561 gcagctcgtc aacacgggcg acggcttcca catgtggtcc gagacgtacg atcgcgagtt
     1621 gggcgacgtc ttcgcaatcc aggaggagat cgccaacgag gtggtaagag aactcaaggt
     1681 gcggttgttg caggaggaaa gcgcgcagtt ggcacgaagg ccgacggaca acttggatgc
     1741 gtacgaagct taccttcgcg gcaagcacga aatggcccgc cgcaccagtg ccgcgctgca
     1801 aagcgctgcg gatcatttca aacgcgccgt cgccttggat cccgagttcg cgctcgcgta
     1861 cgtcggcctt gccgacgcct atcgtcttca cggccactac ggggtggctt cgtacgacga
     1921 tttcgttccc gagatcgagc gcttgatcaa taaggcgatc gagatcgacg accgcttggg
     1981 cgaagcctat gcatcgctcg cagttctgcg gggcatccag gatcgatggg cggaagtggc
     2041 cccgctcttt gagcgcgcaa tcgaactggc tccgagctac gccaccgcct atcactggta
     2101 tggagggtcg ttgtcagatt ccgataccga ccccgccaag gctggcgcca tgcttcggaa
     2161 ggcggtcgag ctggacccgc tttcgccggt gatcaacctg gcctattcgg aacatctcca
     2221 acagatcggc cagttagacg aggccctggg gcgaatcgag cgagctttgg agctcgacga
     2281 ctcgtttccg ggtgcatacg ccagtttggc gaacctgtac taccgcgaat acgggcggat
     2341 cgacgaggcg ctggggtcga tcgatcgggc catcgcgctc gaccccggca acatagggct
     2401 actctacggt cgcgcggatt atctcgccgg ggcgggtcgc gtcgacgaag caatcgccgc
     2461 gtgccgcgcc gcggtcgacg ctcatccgga tttcgcatgg ggatacaaga tggcgggcga
     2521 cgtgcatcgc aaactcggcc gcctcgacga agcgctgtcg ttctatcggc gggccgccga
     2581 gattgagcgc gaggacttct ggggggagtt ctactgcgcg ttgaccctgt tcagtatggg
     2641 tgacgaggaa gcggtcgaag cgtacatcgg gcgcatgcca ccgatacagt ccgggatcat
     2701 cgaagtcgta tatcgtcttt gccgcgtgga gttgggacgc gcgaatgaga atctggaaga
     2761 gctggtcgcg ctggcggggt ccgattttct caacgacacg gtcgccgatg tcgccctcgc
     2821 cgagggacgc tatgccgacg cgatggggac gtacgagcag gcgtttccgg agttactccg
     2881 agattccgac gtgcgcgttc attcgggcaa cctcgatccg gctattcgca tcgccgcggc
     2941 acgaattgga attggcgaga ccgaacgcgc gcgctggctt attgggaaat gcgcgaccta
     3001 tctcgaatcg ctccccgaaa ccgaacgcca ctatgagttc ggccggcaac tcgcggaagt
     3061 gtatgcgctc cagggccgca agcgggaggc actcgacacg ctcgagggcc gggtcgacgc
     3121 ggggctgcgg gacgagtggt gtacccagat gcgctcgccc atgctggttt cgattcgcga
     3181 cgatccggag ttcgtggccg tcttcgacga agtgaagaac gacgtcgcgg gcatgagagc
     3241 acgcctgacg gcgacggatc ggacgccgcc gtgagcccat gtccccgttg agatatcgag
     3301 tcgatcgcgc tatggttgac ctatgagcac tcgcagctct ctccgcctcg ggatggccgt
     3361 gttgcttgct ttgctcgcgt cgctcgacgt gcgcgcgcag gaagccatcg aggtacgtga
     3421 ctcggtgatc gcgcccgtgc aaggcatctc tcacgcgatc gagagcaccg tcgcgttctt
     3481 catcgacgag aaagatctcc ttcccgagag cgtcgcctac gatcccaagg ataagtcgtt
     3541 ctatgtcggc agcacccgca agggcaagat cgtgcgcgtg gacaaggacg gcaacgcgag
     3601 cgacttcatc gcgtcgcgcc aggacgggtt gtggcaggtg atcggcatca agatcaaccc
     3661 gacgcgccgt attctgtggg cgtgcagctt cgacggcgaa agcctcgagg gcttcaagcc
     3721 gggcgacacg cgcgccaccg gggtgttcgc gttcaatctg gataccggta cgctggtgcg
     3781 caagtggacg atcgagaagc cgggcgaggt gcacgcgttc aacgacctgg tcgtgacgcg
     3841 cggcaacgac gcctacgtga cccacatgtt cgacgacgcg gcgatctata agatcaccga
     3901 caggacgcag tcacttgagg tgttggcacg gccggcgggg ttgaaggacc ccaacggcat
     3961 caccataaca cccaacgagg cgactctctt cgtcgcgggg gcggatggga tctacgccgt
     4021 cgatcgcgcg acgggggcgt cgcgcccgct gcaagccgct gagggcgacc cgttgggcgc
     4081 catcgacggc ctctatttct accggggcag tttgatcgcg gtgcacatga ccagcgtgcg
     4141 gcgccatcgc ctcgacaacg caaacacgcg cgtggtgagc acggagatcc tcgaagccga
     4201 tcatccgctc ttcgacgttc ccaccaccgg cgtcatcgtc ggcgacgact tctactacgt
     4261 cgccaactcg cagttcagcg cagtccagaa ggacggaaca ctgctcccga tggatcagtt
     4321 gaacgagccc gcgattctca agctgcgact ccgctagtcc gcctatttca agagcacgag
     4381 cttgcgcgcc tgctgaaagc ccggcgcctc caggcgatag aagtagaccc cactcgcgag
     4441 cccgctcgcg tcgagctcga cgcggtgcgc gcccgccggc agcgatcggt cgatcaccgt
     4501 catgacgcgg actccggttg cgtcgaagac gtgcagccga acgtgcgtat cgctcggaat
     4561 cgcaaacgcg atcgtggtgc gcgggttgaa gggattgggg tagttctgct cgaggcggaa
     4621 gacgcgggcg cgcggcgttt ccacaccggt cccgatcggg ttcgtcgacg cccagaaacc
     4681 cccgaccacc tggaagctcg ctcccgtggc cccgccgacg gcgggctggc cggccgtgcc
     4741 gaccagcgcg tagctcgtgc tcgccgcctg cgtaccgccg gacccgacca cgaagcgaga
     4801 gatcacggcc tgcgcgtgcg cgctccccgc gaacgcgcag agcagcgtcg cgacgactgc
     4861 gacgcgcgcg ctcatggcag tggctccgtg acggtatacg tgatgcgcac ggccctcaag
     4921 gacaagttga ggccggcggc ggtcgaccag aacgcctgca gcgagtaagc ccagttctgg
     4981 ttgtccacga tatcgctggg aaggaagaga tttctcgtgg cgatggtgag cccggcggct
     5041 tccggaatcc cgctcgcgac gacgccgagg gtttggaggg ttccgtcggt gacgttgacg
     5101 cggcgcagcc ccaccgacag ctcgagatcc gggatcgcgt cttcgaacag aacgtggagc
     5161 tcggtcagtt gcgcgccgtg gggcagggtc agtacggcac ccatcgaaat gttggtaccc
     5221 gccgtgggat acacgcgcac cgagttcttc gtgtacgcgg ttgcgctgct gttgggcgtg
     5281 aacgcttccg cgcagacggt gtggtagcgc gtcgtggggc tcatcgtgac cgactccgcg
     5341 gtggtcgcgt gcgccgcggt caaggcgtac ggcacgctcg tgagcggcgt ccacggggac
     5401 agctcggagt cgtcgccaac tttgattccg agcgagagcg gtgccccctg cagaatcgcc
     5461 gggtcgatcg gaaccacgct ccccaagagc accgagaatc ggccgctcgt caccgtcacc
     5521 gtctgggtct cctcccacac cagcgccggg tcgtagagcc gaaagcgaat gctgtagtcg
     5581 tcgtcgggga cgggggtacc cacggcgtcg gtgagcagcc cctgatagct gatggtcccg
     5641 ggcacgtccg cgcgcgcggc cggcacgaac gcgagaagga cccaggccag ggtgagcacg
     5701 atcctcatgg cgaacctcca ggttgtgttg gagagacacc tgaaccggag cggcgtcaca
     5761 cggcgcgcgc ggtaatttgc ggcgaactag ggccaaatag cggagcccgg ccgccgagtg
     5821 agacggactc gacgaccggg ctcctggtcg gcttggaagc ccaagcgctt tacttcagga
     5881 gcaccatctt ccgctgctgg acgaagtcac ccgcccgcaa ccgatagaag tacacccccg
     5941 agctcaagcg cgacgcgtcg aagtcgaccg tgtgccggcc ggctcccttc gactcgtcga
     6001 gcagcctggc caccaaccgg cccgccgtgt cgaagacctc cagcgtgacg tcgctctccg
     6061 acgccagcgc gaacgcgatc gtcgtgcgcg ggttgaacgg gttggggtag ttctgctcga
     6121 gtgtggtctc gaacgacacc gcctccgacg tgatgtcgat cgggcggtcg ccttcgcgga
     6181 cgaggacggg ttccgcgatc tcgctcgacg cgcccaccgt gggcgtggca cgccacgtga
     6241 tctcatccac gtacacgtcg tcgttgttgt cgcccgcgtc gcagcggaag cgaatccgcg
     6301 cgttggtcgg gaaggtgtac gtgcctcggt tcagcgtgat cgtagccgtg tagaacgacc
     6361 cgttggcgaa gctcgtcccc gccacgtagt tggcgacgat ctggtacgcg ctcccgttgt
     6421 agtactcgac gtagaagttg tcgccggtct ccatgctctg agcacggaag aagaacgtga
     6481 tctgctgcgt ctggtacgcg gtcaggttgc gggccgtggt ggtggagaac gacgagggcg
     6541 tgccgctgtt gtcctggatg tcgatcgacg ccacaccctg gtacgagaac gtcccgccgg
     6601 tgtagcgcga gcagtccacg ccgccgtcgg tgtagctccc gaagttggtc tcgaactggt
     6661 cgaaggtgat cgtggtccag accaccccac cgctcgtcac cgagacatac gcgctcttga
     6721 cctcggcgtc gctgccgcag gcattggtcg ccgtcagcgt gacggtgtag gtcccggccg
     6781 ccgcgtaggc gtggctcggg ttctgtgccg tcgacgtccc gccgtcgccg aagttccagc
     6841 tccagctggt cgggctgccc gtcgacaagt cggtgaagtt caccgtcagc ggtgcggtcc
     6901 ccgaggtcgg cgtgcccgag aagttggccg tcacgagcgg cgagcaaccg ccgccaccgc
     6961 cgaccgcggc attgat
//
LOCUS       DASPGK010000385         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1029831, whole genome shotgun sequence.
ACCESSION   DASPGK010000385 DASPGK010000000
VERSION     DASPGK010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029831"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VEC56_10595"
     CDS             complement(<1..735)
                     /locus_tag="VEC56_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HXV14639.1"
                     /translation="MTLRRFSTHALRFVPLLLLALLIAPAAAWADETCMSPYMTKITG
                     QEEFVYVWTLGMDKLGDGSDKVVTVDVRPGSKTFGKVLSTASVGGRHEAHHGGFTDDR
                     RQLWLGGLDTSKIFVFDVATDPAKPKLVKTIDSFVKDSGGVVGPHGHYALPGRMMIAA
                     LSNNKDHSGRTALVEYTNEGKYVATHWMPIDGDTYGAKVEKVADGYGYDLRALPRKNI
                     MLSSSFTGWSNYMMDVGKVISDPEAMK"
     gene            complement(889..1884)
                     /locus_tag="VEC56_10600"
     CDS             complement(889..1884)
                     /locus_tag="VEC56_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14640.1"
                     /translation="MLNAQAAAAQQFQIGIIDFYGLRRVSASESRRALTFAEGDSISL
                     EGDEPPAFLAESERRLATLPGVVRARTNIVCCDQGRLIVYVGVEEEGGATLRFRAAPQ
                     GTARLPADLVQAGDDYWAAFTTAVERGDAGEDGSRGHALCNDPTTRAVQERFVAYAAR
                     DTERLRGVLRESSNADQRALAAQVLGYVADKQSVVDDLVYGMSDPSENVRNNSMRTLM
                     VFTRMTPAAGTSVPRVPYAPFVNLLHSLVWTDRNKSSLALMELTESRDADLLERLRNE
                     AMQSLADIARWKNEGHATPGLLMLGRIAGWADDDVRSAWRSGQREKIIAAALAAR"
BASE COUNT          370 a          678 c          645 g          382 t
ORIGIN      
        1 cttcatggct tccgggtcgg agataacctt gccgacgtcc atcatgtaat tcgaccagcc
       61 cgtgaacgac gacgagagca tgatgttctt gcgcgggagc gcgcgcaaat cgtaaccata
      121 accgtcggcc accttctcga ccttggcgcc gtaggtgtcg ccgtcgatcg gcatccagtg
      181 cgtagccacg tacttgcctt cgttggtgta ctccaccaaa gcggtgcggc cgctgtggtc
      241 cttgttgttg gaaagtgccg cgatcatcat gcgtcccgga agcgcgtagt gaccatgcgg
      301 tcctaccacg ccgccggaat ccttgacgaa gctgtcgatg gtcttgacga gcttcggttt
      361 ggcggggtcg gtggcgacgt caaacacgaa gatcttgctc gtgtcgagtc cgccgagcca
      421 caactgccgg cggtcgtcgg tgaaaccgcc gtggtgcgct tcgtggcgcc ccccgaccga
      481 cgctgtgctg aggacttttc cgaacgtctt cgatccgggg cgcacgtcga cggtgacgac
      541 tttgtcggac ccgtcaccga gcttgtccat tcccagcgtc cacacataga cgaactcttc
      601 ctgacccgtg attttggtca tgtacggaga catgcaggtc tcgtcggccc acgccgccgc
      661 cggggcgatc aacagggcca aaagcaatag cgggacaaat cggagcgcat gcgtcgaaaa
      721 gcggcgcagg gtcatggtcg acctcctgga tgcgaccgac ggcagccggt caatatgggt
      781 tgggtgaaaa cgaacggccg gattctagca cggcgagcac tcaccggcaa gccggttagg
      841 ggaaaatccg cggggcgccg aaccacgcgg cgggccggcg ggacgcgctc aacgggccgc
      901 gagcgcggcg gcgatgattt tctcgcgctg gccgctcctc catgccgacc tcacgtcgtc
      961 gtccgcccag ccggcaatcc gtccgagcat caggagaccc ggcgtcgcgt gaccttcatt
     1021 tttccatcgc gcgatgtcgg cgagggactg catcgcttcg tttcgcagtc tctccaagag
     1081 atcggcatcc cggctctccg tgagttccat gagcgcgagc gacgacttgt tgcgatcggt
     1141 ccaaaccagc gagtggagca ggttgacaaa aggcgcatac ggaacacgag gcacggacgt
     1201 tccggcggcc ggcgtcatcc tcgtgaacac catgagcgtt cgcatggaat tgttgcgcac
     1261 attttcggat ggatcgctca ttccatacac gaggtcgtct acgacgctct gtttatcggc
     1321 gacgtatccc aggacttgtg cggcgagcgc gcgttgatcc gcattcgacg actcgcgcag
     1381 gacgccacgg agtcgctccg tgtcgcgagc cgcgtacgcg acgaagcgct cctgcaccgc
     1441 tcgtgttgtc ggatcgttgc acagcgcgtg gccccgagac ccgtcctcgc cggcatctcc
     1501 ccgctcgacg gcggtcgtga acgccgccca atagtcgtcg cccgcctgga cgaggtcggc
     1561 cggcaagcgg gcggttcctt gcggggcggc gcgaaaacgc agcgttgctc ctccctcctc
     1621 ctcgacaccc acataaacga tgaggcggcc ctggtcgcaa cacacaatgt tggtacgcgc
     1681 cctcaccact ccgggcaggg tcgcgaggcg ccgctccgac tccgccagga atgcgggcgg
     1741 ctcgtcgccc tcgagcgaaa tactgtcgcc ctcggcgaac gtcagcgcgc gacgcgattc
     1801 gctcgctgaa acgcgacgca gtccatagaa gtcgatgatg ccgatctgga actgctgggc
     1861 cgcggccgcc tgtgcgttga gcacaacaac gaagcctgcg acgccatttg ccatctttcg
     1921 tcgggtcacc ggtacagcgc cttgaccgct ccccacgtgc ttgcccgggt ggggagcggg
     1981 ttcttcccga ggaccacgac cattccgatt cggtaatcgc cggaacccac gaccactccg
     2041 tgcaccggat aggggtgtac gcccagcaat cgaat
//
LOCUS       DASPGK010000386         4821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_211821, whole genome shotgun sequence.
ACCESSION   DASPGK010000386 DASPGK010000000
VERSION     DASPGK010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211821"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1212)
                     /locus_tag="VEC56_10605"
     CDS             complement(184..1212)
                     /locus_tag="VEC56_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HXV14641.1"
                     /translation="MRNVAKRTMWVLAGVAGLALVATDSVRSQGQPKIDIPETRFDFG
                     RVFERKQYIHDFIVYNRGDADLIINNVRPSCGCTVTNFDKLIAPGKQGKIEFVLDGEK
                     VHNEFSKNATVSSNDPTHPTMTIAVSGNEIPYLNIDPAGTVYLHGRYGEHIERTMTVS
                     SNEENLDFRILRASSNIDDKITYQVEQAATPGTYQVTVYKNPQLPTLITYGTLYLHTN
                     SKVSPKSEIQVHIITKGDITVSPQAVNFGQVKFAEGAGAGQSVTRSIIVSKSAGEFSI
                     KDVKLSNPNFSAIVEPVSEGKQYRVQVTFTPPLKTLVQQNETSEMIIHTNDAQEPAIR
                     VAVAARAM"
     gene            complement(1222..2283)
                     /locus_tag="VEC56_10610"
     CDS             complement(1222..2283)
                     /locus_tag="VEC56_10610"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HXV14642.1"
                     /translation="MHRRMVASIGVLTFVLAIAGAVIAQEASKPAPAKPAATGPRPII
                     EIPKMRQDFGEVYERDKFEYSFVVKNRGKADLVISEVKPGCGCTVANFDKVIPPGGEG
                     KIELVLDGTRVHGEFSKTAQVHSNDPDHPELTLTIAGKKVAFLNVVPDGVVYLQGRYE
                     EVVEKEVTISSNEKLPDFKVIGVTSDIDDKISYDVKPAAKKGEYTLSIKKNPDLPMST
                     SYGSVTIKTNSTNAPETKLQVQVMTKGNITVSPSSVNFGNVAFAAKDKNAAPVTRTVM
                     VIKNSGEGLDVKDVTVDNPNFKAKVEPVTPGKQFKVEVTFSPPLKRTSSQRETGELII
                     NTNDPREPSVRVHLVARAM"
     gene            2531..3493
                     /locus_tag="VEC56_10615"
     CDS             2531..3493
                     /locus_tag="VEC56_10615"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV14643.1"
                     /translation="MSSIPLRLVRPNQGSESESILSKIGRTPLFRLERIDREFPNASI
                     YAKAEWLNPGGSVKDRPALRMILAAEQSGRLRPGQTVLDATSGNTGIGYAMIAAARGY
                     RVHLTMPANASAERIRILRAYGAELTLTDPALGVDGAIEAAQALHAASPERYCYLNQY
                     DNDDNWRAHYEGTAWEIWNQTAGCVTHFVAVLGTSGSFMGTARRLRELNPRIACISVQ
                     PDSAFHGLEGMKHMPSARVPAIYDASLADRNIEVHTEAAYRAVKRLIREEGMLVGISS
                     GAAVVACLQIARALPAGRPAMIVTIFPDNGEKYLNERFWEEVSE"
     gene            3490..3942
                     /locus_tag="VEC56_10620"
     CDS             3490..3942
                     /locus_tag="VEC56_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HXV14644.1"
                     /translation="MIRIAPELIAAIRAHAERDFPSECCGALLGRAGSAPSPASTKWV
                     LAIEPVENRRETEAASRRFLITADDYRAIERMARAKSLDVLGFYHSHPNQPARPSDYD
                     REHALPSYSYMIVSVRDGRAGELTSWTLDDDRVSFTPESVAEIASASR"
     gene            3948..>4821
                     /locus_tag="VEC56_10625"
     CDS             3948..>4821
                     /locus_tag="VEC56_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXV14645.1"
                     /translation="MATQIMIPSALRRFVGARDSVQVEAATVGEALARLVAEYRDLHK
                     HLYDENGRLRSFVNIYVNDEDVRYLQKDDTRLAPGDVVSIVPSIAGGSPKPNVYAVEG
                     LSTDEVKRYSRHLIMPEVAIDGQLKLKAAKVLLIGTGGLGAPAGLYLTAAGVGKIGLV
                     DFDVVDYTNLQRQVTFGTKDVGRAKLAAARDRLGDLNPEVEFVLHETRLTSDNALDII
                     RDYDIVVDGTDNFPTRYLVNDACVLLGKPYVYGSIFRFEGQITVFAAADGPCYRCLYP
                     SPPPPGLVPSCAEGG"
BASE COUNT          798 a         1567 c         1524 g          932 t
ORIGIN      
        1 cggtcccggc gctcgctgag accggaacgc ccagcggctt ggtctcttcg gcctgcacct
       61 gcgcggtcgc gacctccgtg ggagtggcgg ccttgtcctt ggcgctcagc agcacgacgg
      121 gcaccacgat cgcaatcgcc agcgccgccc cgatccatac gcgtcgattc attcggttct
      181 ccgttacatc gcacgcgcgg ctacggccac gcgaatggcc ggctcctgcg catcgttggt
      241 gtgaatgatc atctcggagg tctcgttctg ctgcaccagc gtcttgagcg gcggcgtgaa
      301 cgtgacctgc acccgatact gcttgccctc cgacacgggt tcgacgatgg ccgagaagtt
      361 cgggttgctc agcttgacgt ccttgatcga gaactcgccc gccgacttgg agacgatgat
      421 gctgcgcgtg accgattgcc cggcgcccgc gccctcggcg aacttcacct gcccgaagtt
      481 cacggcctgt gggctcaccg tgatatcgcc cttggtaatg atgtgcacct ggatctccga
      541 cttcggactc accttactgt tggtgtgcag gtacagcgtc ccgtaggtga tgagcgtggg
      601 cagttgcgga ttcttgtaga ccgtcacctg gtacgtgccg ggcgtcgccg cctgctccac
      661 ctggtacgtg atcttatcgt cgatgttcga cgacgcgcgc aggatcctga aatcgagatt
      721 ctcttcattc gacgacaccg tcatcgtacg ttcgatgtgc tcgccgtagc ggccgtgcaa
      781 gtacaccgta ccggcggggt cgatgttgag atatggaatc tcgttgcccg aaaccgcgat
      841 cgtcatggtg ggatgcgtgg ggtcgttgga gctgacggtc gcgttcttgc tgaactcgtt
      901 gtgcactttc tccccgtcga ggacgaactc gatcttcccc tgcttgccgg gcgcgatcag
      961 cttgtcgaag ttggtcacgg tgcacccgca gctgggtcgt acgttgttaa tgatcaagtc
     1021 cgcgtcgccg cggttgtaca cgatgaagtc gtggatgtac tgcttgcgct cgaacacccg
     1081 gccgaaatcg aagcgggtct ccgggatgtc gatcttgggc tggccctgcg accgcaccga
     1141 atcggtcgcg accagcgcga ggccggcgac gccggccagc acccacatgg tccgcttcgc
     1201 gacgttgcgc atgggacgtc cttacatggc ccgcgccacc aggtgcacgc gcaccgacgg
     1261 ctcgcggggg tcgttggtgt tgatgatgag ttcgccggtc tcccgctgcg acgaggtgcg
     1321 cttgagcggc ggcgagaacg tgacttcgac cttgaactgc ttgcccggcg tcaccggctc
     1381 taccttggcc ttgaagttgg ggttgtcgac cgtgacatcc ttcacgtcga ggccctcgcc
     1441 ggagttcttg atcaccatga cggtgcgcgt gaccggggcc gcattcttgt ccttggcggc
     1501 gaacgcgacg ttgccaaagt tcaccgacga cgggctcacg gtgatgttgc ccttggtcat
     1561 gacctgcact tggagtttgg tctcgggcgc gttggtgctg ttggtcttga tggtgacgct
     1621 gccgtaagac gtgctcatgg gaaggtcggg gtttttcttg atgctcagcg tgtactcgcc
     1681 cttcttggcc gccggcttga cgtcgtagct gatcttgtcg tcgatgtcgg aggtgactcc
     1741 gatcactttg aagtcgggaa gcttctcgtt cgacgagatg gtcacttcct tctcgacgac
     1801 ttcctcgtaa cggccctgca aatacacgac accgtcgggg accacattga gaaacgcgac
     1861 cttcttgccc gcgatggtga gcgtgagctc ggggtgatcg gggtcgttgg agtgaacctg
     1921 cgcggtcttg ctgaactcgc cgtggacgcg ggtgccgtcg agcacgagct cgatcttgcc
     1981 ctcgccgccg ggtggaatca ccttgtcgaa gttcgccacc gtgcatccgc agcccggctt
     2041 cacttcggag atgacgaggt cggccttgcc gcggttcttc acgacgaagg aatactcgaa
     2101 cttgtcgcgc tcgtagactt ccccgaagtc ctggcgcatc ttgggaatct cgatgatcgg
     2161 ccgggggccg gtcgcggccg gtttggccgg agccggcttc gacgcttcct gggcgatgac
     2221 cgcccccgcg atggcgagga cgaaggtcaa tacgccgata ctcgcaacca tccgcctgtg
     2281 catggctact cctcgaaact tgtttcggtg caatcggtaa aagggcccgg gtgaacgcca
     2341 cccatggggt cagggacacc gtattatacg cgccgaaggc gtccggttcc acgctgtttt
     2401 gagcaggaat cacgccgacg cagacccctc ccgttgcgga atccgttgcc ccgtggtatg
     2461 ttatcgctgc gcctgagaga taaccggggg aaatagcacc cagcgagtcc gagatcatgc
     2521 ccaacctgcc atgtcgtcga ttccattacg cctcgttcgg cctaaccagg gctccgaaag
     2581 cgagtcgatc ctttccaaga tcggccgcac gccgctgttt cgcttagagc gaatcgaccg
     2641 cgagtttccc aacgcctcga tatacgccaa ggccgagtgg ctcaaccccg gcgggtccgt
     2701 gaaggaccgg cccgcgctgc gcatgatcct cgcggccgag caatcggggc ggcttcggcc
     2761 cgggcaaacc gtactggacg cgacctccgg aaacacgggc atcggctacg cgatgatcgc
     2821 cgccgcgcgc ggctaccgtg tgcatctgac aatgcccgcc aacgcgagtg ccgagcgcat
     2881 tcgcatcctg cgcgcgtacg gggcggagct gaccctcacc gatcccgcgc tcggcgtcga
     2941 cggcgcaatc gaggccgcgc aagcgctcca tgccgcgtcg cccgagcgtt actgctatct
     3001 caatcagtac gacaacgacg acaactggcg cgcccactat gaaggcaccg cgtgggagat
     3061 ctggaaccaa accgcagggt gcgtgacgca cttcgtcgcg gtcttgggca ccagcgggtc
     3121 gttcatggga accgcgcggc gcttgcgcga gctcaatccg cgcatcgcgt gcatctcggt
     3181 gcaacccgac tcggccttcc acggcctcga gggtatgaag cacatgccgt ccgcgcgcgt
     3241 tccggcgatt tacgacgcgt cgctcgccga ccgtaacatc gaagtccata ccgaggccgc
     3301 gtatcgcgcg gtcaagcgcc tgatccgcga ggaaggcatg ctggtcggga tctcgtcggg
     3361 cgccgcggtg gtcgcgtgct tgcagatcgc gcgcgcgctt ccggccggcc ggcccgcgat
     3421 gatcgtcacc atcttccccg acaacgggga gaagtacctg aacgagcgtt tctgggagga
     3481 ggtctcggaa tgatccgcat cgcgcccgag ttgatcgccg cgattcgtgc ccacgccgag
     3541 cgcgatttcc ccagcgagtg ctgcggcgcg ctcttgggtc gcgcgggctc ggccccgtcg
     3601 ccggcctcga ccaagtgggt gctcgcgatc gagccggtcg aaaaccggcg cgagaccgaa
     3661 gccgcgtcgc gccgctttct catcaccgcc gacgactacc gcgcgatcga acgaatggcg
     3721 cgggccaagt cgctcgacgt cctcggcttc tatcattcgc acccgaacca gcccgcgcgc
     3781 ccgtcggact acgaccgcga gcacgcgctt ccatcctact cgtacatgat cgtctccgtg
     3841 cgcgacggcc gcgccggcga gctgaccagc tggacgctcg acgacgaccg cgtctcgttc
     3901 accccggaat ccgtcgcgga gatcgcctcc gcaagcaggt gagatcgatg gccacgcaga
     3961 tcatgattcc ctccgcgctc cggcgtttcg tcggtgcgcg cgactcggta caggtggaag
     4021 ccgcgaccgt gggcgaagcg ctcgcgcgtc tggtcgccga ataccgcgac ctccacaagc
     4081 acctctacga cgaaaacggg cgcctgcgca gcttcgtcaa tatctacgtc aacgacgagg
     4141 acgtgcgcta cctgcagaag gacgatacgc gcctcgcgcc gggagacgtc gtcagcatcg
     4201 tgccgtcgat cgcgggcggg tcgcccaaac cgaacgtgta cgcggtcgag ggcttgtcga
     4261 ccgacgaagt gaaacgctac agccgccacc tcatcatgcc cgaagtcgcg atcgacggtc
     4321 agttgaagct caaagccgcg aaagtgctct tgatcgggac gggcggtttg ggggcacccg
     4381 cggggctgta tctcaccgcg gccggcgtgg gcaagatcgg cttggtcgac ttcgacgtgg
     4441 tcgactacac gaatctgcag cgccaggtca ccttcgggac caaggacgtg ggccgcgcca
     4501 agctggccgc agcgcgcgat cgcttgggcg acttgaaccc cgaggtcgag ttcgtcctgc
     4561 acgagacgcg gctcaccagc gacaacgcgt tggacatcat tcgcgactat gatatcgtcg
     4621 tcgacggcac ggacaatttc ccaacgcgct atctcgtcaa cgacgcgtgc gtcttgctcg
     4681 ggaagccata cgtgtacggg agcatctttc gcttcgaagg acaaatcacc gtcttcgccg
     4741 cggcagacgg cccgtgctac cgctgcttgt atccctcgcc tccgccgccg gggctggtcc
     4801 cgagctgcgc ggaagggggc g
//
LOCUS       DASPGK010000387         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1617556, whole genome shotgun sequence.
ACCESSION   DASPGK010000387 DASPGK010000000
VERSION     DASPGK010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617556"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /locus_tag="VEC56_10630"
     CDS             complement(<1..1953)
                     /locus_tag="VEC56_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HXV14646.1"
                     /translation="MVSATAVVRARAVRELGLRRGGESWTIWRGHWLGRIMKFSFFGI
                     VLFVACALVAATASVAPAQEDVSERARWGFYFNLGAGDTRGDFDPAFQKAATGEFGIV
                     RQRGVWRYGLGASFGSFGMEPPYDNELEWGFQRIFVSGTRVFRADQGVRPYLQARLGV
                     ARLHPRSLLFEMDPLPDDFEIGDSPTKASNGVHVGVVPGLEWQLSNAVALDFSLMLDY
                     FVVGDTDLSPIGLGSASSGFDWQARLGVLWWTDGGASTESAERDAWGVRPSYGWALGE
                     ALAINFGASALNEYVRNANFNQVSPRSWLSNLEEGFTYDDNEFSTNQFIHPFNGSQYY
                     NAARSNGLSFWPSYGIALIGALHWEWMGETHPMSFNDLVSTGIGGAAMGETMYRFSSM
                     MLDNEATGMRRFWREAGSFITDPIRGFNRLVSGRAWKVSDNPTDRMDTNPIGQFNGLS
                     VGWRRIGNGNYPSLSHDVSDHGFVEYLHEHGNVWDNDRRGPWDYFLFDGQINMGDKTK
                     FGQLIMQGNWVSTPIGESDPVKHMGAFIQLFEYINSNAYEYGGQSVGASVYSRWGSSG
                     RLSFDTRFDLLGTIMGAVNSNAASLADVADPERLREYDYGPGAGGRALLELKWLRKPF
                     VTLGYRLNFLYVTNGSVWSGTDQDTGE"
     gene            1952..3247
                     /locus_tag="VEC56_10635"
     CDS             1952..3247
                     /locus_tag="VEC56_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14647.1"
                     /translation="MVALATPARAQDAHYWSLQVGSRATLLGGSVVALDTDLSAAYYN
                     PGSLVRVAEGSALSMFAKTVTTLTLSLDTSTPLAAESDIGASAPGMFAARVPGVHLFE
                     DDVITFSYLVRQSSKLDLTGAVLSSPTVPAEALDFFAFQDIYDGWYGLSWARDVGGFG
                     AGVSVFFSSVSYRQRLETKDIVLESASEAGALSENVYYSFACRRVVAKGGVSWTGGPL
                     SLGASLTLPSLRLPWSSGTMSVGRNLVAQDSVLVSQIAFSRQEDLDADYREPIAVGLG
                     AQFELGSFGFYAAAEWFGSVDEYDVLETRPLTSQVPPQEFVLPLTQRREDVLNIGGGV
                     SVEATSWLSFYGSVRTDRSYRDPADRTFVGLGAYDITQVTFGIGVSSDEFEVILGGLA
                     GEGDSDGQVVLSPLPGSPSVASRTEFEQRGFIVAFSASF"
     gene            3256..4287
                     /locus_tag="VEC56_10640"
     CDS             3256..4287
                     /locus_tag="VEC56_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXV14648.1"
                     /translation="MRRHTPWIAILLASATCLPAPGEARQKTDLVFLSNGDRMTGEIT
                     QLDRGILKLKTNDIGTLSIEWEDVDSLNSVYQFRVEDHAGAKYFGAIFLNKRGVLEVI
                     QGGGSEQVPHEDVVAITPLEASFWQQLDGSIAIGFSYTKSNSLAQLTSDIYVRRRTPI
                     RLWELDISSIETSQSNDETQRRENLSFTYSRLFKGPLFGTSSAGAQTNDELGLDLRVL
                     FSVGAGYSWVQTNHNDFMSSLGLSVNREWSDDANGDYNLEAYVSATHSVFRYDYPKTD
                     ITAEFTVYPNLTNWGRIRGELDVSASREVIKDFTIVLSFYDSYDSDPLSATAAKNDYG
                     LVTSLGWTF"
     gene            complement(4288..>4696)
                     /locus_tag="VEC56_10645"
     CDS             complement(4288..>4696)
                     /locus_tag="VEC56_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14649.1"
                     /translation="WDSGVELVAQYLKDFGENLTYTSRLSFYQPFNWSKKSVFEDASP
                     DSLAAAGVPTDVADYTTTVDIDWQNTVSAKVTKVIAVQLYVELLYDKYDNTVVPEVDD
                     TAMLENPGPVAASVRKKGQFKETLGIGLAYTFK"
BASE COUNT          834 a         1540 c         1456 g          866 t
ORIGIN      
        1 ttcgcccgtg tcctggtcgg tgccgctcca aaccgagccg ttcgtcacgt acaagaaatt
       61 caaccggtag ccgagggtga cgaacggctt gcgcagccac ttgagttcga ggagcgctct
      121 cccgccggcg ccggggccgt aatcgtattc gcggagccgt tccgggtcgg ccacgtccgc
      181 gagtgaggcc gcgttcgaat tgaccgcgcc catgatcgtg ccgagcaggt cgaagcgggt
      241 gtcgaacgac aatcggccgg acgagcccca gcgcgagtac acgctggcgc cgacgctctg
      301 tccgccgtac tcgtacgcat tgctgttgat gtactcgaag agctggatga acgcgcccat
      361 atgcttgacc ggatccgatt cgccgatcgg cgtcgacacc caattgccct gcatgatcag
      421 ctgcccaaac ttcgtcttgt ctcccatgtt gatctgcccg tcgaagagga agtaatccca
      481 ggggccgcgc cgatcgttgt cccagacgtt gccgtgctcg tgaagatact ccacgaatcc
      541 gtggtcgctg acgtcatggg aaagcgacgg gtagtttccg tttccgattc tccgccatcc
      601 cacgctcaaa ccgttgaact gtccgatggg gttggtgtcc atgcggtcgg tcgggttgtc
      661 gctcaccttc cacgcgcgcc cggacaccag gcggttgaac ccgcgaatgg gatccgtgat
      721 gaagctgccc gcctcgcgcc agaagcggcg catgccggtc gcttcgttgt cgagcatcat
      781 cgacgaaaag cggtacatgg tctcgcccat cgccgcgccg ccgatacccg tcgaaacaag
      841 atcgttgaat gacatcgggt gcgtctcgcc catccactcc cagtggagcg cgccgattag
      901 cgcgatacca tacgagggcc agaacgagag gccgttggag cgcgccgcgt tgtagtactg
      961 cgagccgttg aagggatgga tgaactgatt ggtggagaac tcgttgtcgt cgtaggtgaa
     1021 accttcctcc agattcgaca gccagctgcg cgggctgacc tggttgaagt tcgcgttgcg
     1081 cacgtactcg ttcagcgccg aggcgccgaa gttgatggcc agggcctcgc ccaacgccca
     1141 accgtacgag ggccgcacgc cccacgcgtc gcgctccgca gactcggtac tcgccccgcc
     1201 gtcggtccac cacaagacgc caaggcgcgc ttgccagtcg aacccggacg acgccgagcc
     1261 caatccgatg ggactcaagt cggtgtcacc gaccacgaaa tagtcgagca tgagtgagaa
     1321 gtccagcgcg acggcattgg aaagctgcca ctcgagtccc ggtacgacgc cgacatggac
     1381 gccgttggac gccttggtgg ggctgtcgcc gatctcgaaa tcgtcgggca gcggatccat
     1441 ttcgaacaag aggctgcggg gatggagacg cgccacaccg aggcgggctt gcaggtatgg
     1501 tcgcaccccc tggtcggcgc gaaacacgcg cgtgccggaa acgaaaatgc gctggaagcc
     1561 ccactcgagc tcgttgtcgt agggcggctc cattccgaac gaaccgaagc tcgcgcccag
     1621 gccgtagcgc cacacgccgc gctggcgcac gatcccgaac tcgcccgtgg ccgctttttg
     1681 gaaggccggg tcgaaatcgc cccgggtatc gcccgcgccg agattgaagt agaatcccca
     1741 gcgagcgcgt tcggacacgt cctcttgtgc gggcgcgacc gaagccgtcg ccgcgacgag
     1801 cgcgcacgcg acgaataaca cgattccaaa gaaagagaac ttcatgatgc ggccgagcca
     1861 atgcccacgc cagatagtcc acgactcacc gccacggcgc aaccccaatt cgcgcactgc
     1921 gcgcgcgcgc acgacggcgg tcgcgctgac catggtggcg ctcgcgacgc cggcgcgggc
     1981 ccaggacgcg cactattggt cgctccaggt cggatcgcgg gccacgttgc tgggcggctc
     2041 ggtggtggcc ctcgataccg acttgagcgc cgcgtattac aacccgggtt cgctggtgcg
     2101 cgtcgccgag ggcagcgcgc tgtcgatgtt cgccaagacg gtcactaccc tcacgctctc
     2161 gctcgataca tcgacgccgc tcgcggccga gtccgacatc ggcgcgtccg cgccgggcat
     2221 gtttgcggcg cgcgttccgg gggtgcatct cttcgaggac gacgtgatca cgttctcgta
     2281 tctggtgcgg cagtcgtcca agctcgacct gacgggcgcg gtcctgtcgt cgcccacggt
     2341 ccccgcggag gcgctcgatt tcttcgcctt tcaggacatt tacgacgggt ggtacggtct
     2401 gtcgtgggcg cgcgacgtgg gcgggttcgg cgcgggggta tcggtgttct tttccagcgt
     2461 gtcgtaccgg cagcgtctgg agaccaagga catcgtcctc gagtcggcga gcgaggccgg
     2521 cgcgttgagc gagaatgttt actattcctt cgcgtgccgg cgcgtcgtgg ccaagggcgg
     2581 cgtctcctgg accggcggcc ccctctcgtt gggcgcgtcg ctcacgctcc ccagcctgcg
     2641 ccttccctgg agcagcggca ccatgtcggt gggacgcaat ctggtggcgc aggacagcgt
     2701 gctggtctcg cagatcgcct tcagtcgcca agaggacttg gacgccgact accgcgagcc
     2761 catcgcggtc gggctgggcg cgcagttcga gctgggctcg ttcggcttct acgccgccgc
     2821 cgagtggttc ggttccgtcg acgagtacga cgtgctggag acgcggcccc tcacgagcca
     2881 ggtgccgccg caggagttcg tgctccccct cacgcagcgc cgcgaggacg tgctgaatat
     2941 cggcggcggt gtttcggtgg aggccacgtc gtggctgtcg ttctacggat ccgtacggac
     3001 cgaccgcagc taccgcgatc ccgccgatcg aaccttcgtc ggcttgggcg cgtacgacat
     3061 cactcaggtg accttcggga tcggcgtttc cagcgacgag ttcgaggtga tcctgggcgg
     3121 cctcgccggc gagggcgatt ccgacgggca ggtcgtgttg agcccgctgc ccggctcgcc
     3181 gtcggtcgcc tcgcgtaccg agttcgagca gcgcggcttc atcgtcgcct tcagcgccag
     3241 cttttgaccg tcactatgcg ccgacacaca ccctggatcg cgatcctcct cgcctccgct
     3301 acttgcctgc ccgcgccggg cgaagcgcgc caaaaaaccg acctggtgtt cctcagcaac
     3361 ggcgaccgca tgacgggcga gatcacgcaa ctcgaccgcg gcatcctaaa gctcaagacc
     3421 aacgacatcg ggacgctcag catcgaatgg gaggacgtcg attcgctcaa cagcgtgtac
     3481 cagttccgcg tcgaggatca cgccggggcc aagtacttcg gggcgatctt tctgaacaag
     3541 cgtggcgttc tcgaagtcat tcaaggcgga ggaagcgagc aagtaccgca cgaggacgtg
     3601 gttgccatca cgcccttgga ggcgtcgttt tggcagcagc tcgacgggtc gatcgcgatc
     3661 ggtttcagct acaccaagtc gaacagcctc gctcaactca cgagcgatat ctacgtgcgc
     3721 cggcgcaccc cgattcgtct gtgggagctc gatatcagct ccatcgagac ctcgcagtcg
     3781 aacgacgaga cgcagcggcg cgagaaccta tcattcacct acagccgtct cttcaaggga
     3841 ccgttgttcg ggacgtcgag cgccggcgcg caaaccaacg acgagctcgg cctcgacctc
     3901 cgcgttctgt tctccgtcgg ggccggttac agctgggtgc agaccaatca caacgatttc
     3961 atgtcgtcgc tgggcttgag cgtcaaccga gaatggtccg acgatgccaa cggagattac
     4021 aatttggaag catacgtgtc tgccacccac tccgtgtttc gctacgacta cccgaaaacg
     4081 gacatcaccg ccgaattcac cgtctacccc aatctcacca actggggtcg catccgcggc
     4141 gaactcgatg tcagcgcgag ccgcgaggtc atcaaggact tcaccatcgt gttgtccttc
     4201 tacgactcgt acgacagcga tccactcagc gcaacggcgg ccaagaatga ctacggcctc
     4261 gtcaccagcc tgggctggac gttctagcta cttgaacgtg tacgccagac cgatacccag
     4321 cgtttccttg aactgtcctt tcttgcgtac cgacgccgcc accgggcccg ggttttccaa
     4381 catcgctgta tcgtcgactt ccggcaccac ggtgttgtcg tacttgtcgt agagcagctc
     4441 gacatagagc tgtaccgcga tcaccttggt gaccttcgcc gaaaccgtgt tttgccagtc
     4501 gatgtcgacc gtggtggtgt agtccgcgac gtcggtaggc acgcccgccg ccgccagcga
     4561 atcgggtgac gcgtcctcga acacgctctt cttcgaccag ttgaagggct gatagaagct
     4621 caagcgcgac gtgtacgtca ggttttctcc gaaatccttg aggtactgcg ccaccaactc
     4681 tacgcccgag tcccac
//
LOCUS       DASPGK010000388         9372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_563333, whole genome shotgun sequence.
ACCESSION   DASPGK010000388 DASPGK010000000
VERSION     DASPGK010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563333"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VEC56_10650"
     CDS             <1..1379
                     /locus_tag="VEC56_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV14650.1"
                     /translation="DLGSTNGTYLNGRRVARERLSPGDELIIANVGMLVEASKSSIHD
                     ARPPLNITVSIDQKPTKPSVTMSSAEISGTHEILEGIGADPNAAHRALQVLYNADSAF
                     RNIEDLSLLLDNLLGIIMECIPASRGFVFLIQRDTNQLVPYARRPPGVASDDAEIVVS
                     QTIIQTAVRQKASIISNDALVDERFLHSKSVAALDVRSAMCAPLFNRSNVLGIVYVDS
                     HKANSFSRKDLALFSALALKVAIAVDNARLYDDLRGLFYNTVETLVRTIQAKDQYTAG
                     HSTRVSRYSLMIADKLGLSTKDKHQLYLAAMLHDIGKIGVPDDLLNRPGHLSQEEMER
                     VRNHVVVGASMIEMLGEMHPIVPLIRHHHECYDGSGYPDGLQGEQIPLISRIIAVADM
                     YDAMTSDRPYRKRRTHQFAVEEILSVSGTKIDPKVAEAFLNVLKDIIPGDEKTEPTSE
                     VTSGSPTR"
     gene            complement(1357..2169)
                     /locus_tag="VEC56_10655"
     CDS             complement(1357..2169)
                     /locus_tag="VEC56_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14651.1"
                     /translation="MSIVRFAFLACLCLLAGCSKSSDTADEAKKSPLLELTQKTAAAD
                     QAATLAPVLEARGFRLVTTQRVPAQLSARRAVAAIYRSQDGARGGVVYMQRANNTTEG
                     ITWHWYFADGAPDSLQLVELNGDGLWDARVFMAGGTTRDFIQGETFTLMTDREARFAM
                     NGASSGTDAWKAFDGDTTTAWQSPAKGAFVEIPLPIGLEAGTLSLRVAGGGHVGKVAI
                     YAGDKKVQDVDLKDTGTLRDVPLDAAVKEAPAIRVVVEGRDATVAISELEIR"
     gene            complement(2311..3951)
                     /locus_tag="VEC56_10660"
     CDS             complement(2311..3951)
                     /locus_tag="VEC56_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14652.1"
                     /translation="MSRSLRIVALLALVHALACSRATEPELVPEGRIVMRFEPAALAS
                     AVARDASRATAATTVIDSVVVRVFRPGTPVTQEVAGSAAVGVDPVELSIPCIAENGKR
                     VSVELYQSGYFTHHGYRTGVDVAVGRRTDVTVDAYVFLVASLSVTPSVVVDPESFDLA
                     WTGAPAASRYDVQASGSLDFGTIDWEASVTDTFLTVQLPTGSHYFRVVPRTDYAQGLA
                     SIPEFGYVMGGSGQIAITGFSAPAAIPGDVMTILGENLDFPGTRVWIGSMEMTIVSAS
                     WGSLDVRVPRAATTEYISASSLLGTDTSDDALIVQRVAYVTSSGEWAAEYMEVLFGYW
                     SDYGYSGVAPVPIADLDWRDMSVFDIVIVADDTGTDTGDWGDGVPARALAIAASTANV
                     LALGEGGLAYLQLAVPSISDANSQPRNQTSYYVTTPSASVLTTPHTVTSGGLAQWVDI
                     SSVPERTIGVDIDSASPPAGVNLHACTGLVIVLPNDLWALVDFQLPDIFSNKKRLFYY
                     GHSGSPKRFTKAGADLLGNLMYHLYNDRATVPPVAAAR"
     gene            complement(3948..4583)
                     /locus_tag="VEC56_10665"
     CDS             complement(3948..4583)
                     /locus_tag="VEC56_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14653.1"
                     /translation="MNRTRRITSGLLIVTLFASTVTMPFATAVAGTPGDDLKQIEYKY
                     YFRGKYQQAIESLQTYLARVDLPEPEAMRAREVLAASYVLGGAPAMGKQMFSEMIAKK
                     PSYPGPDPNVFKLEVMDAYAQARSEYAALALKNAPSSAGKNASDRDRGAAALPSDALA
                     EAGKPIYKKWWFYAGAAALIAAVGVAAGGGGDDGTPPPAETGGVTVGVIVH"
     gene            complement(4590..6527)
                     /locus_tag="VEC56_10670"
     CDS             complement(4590..6527)
                     /locus_tag="VEC56_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV14654.1"
                     /translation="MAIRVQIDGYDSLEQIGVGGMAAVYKARKISIDKTVAIKVLFPY
                     LATDASFKERFQREAKAAASIQHENIVNVIDFGESEGSFFIVMEYYQGRTLEDLMKER
                     AGVPFDVATQIILEVAFGLEAAHHLDIVHRDVKPANIIFTDQGGIKVADFGLAKKSDS
                     VTMITQLGKVIGTPAYMSPEQAAGRPVGTASDIFSLGVVAYELFGRRKPFEGKTYSDV
                     LEKIQTYAPPSVIQVNPVIEPDFAAIVSRMLQKNDRDRYPNATALISDLEAAMEKAKI
                     SRDRRRLGSYIKNPDAYDAAVLEKTVANCLSRGTFYLQKGQHHLDDAALEFRRILFLD
                     PNNERARKNLDRIKSQQGGASRTVTIEAATPATASAATTSRVAPVRAGKAIRPARSRR
                     RWVVGVGGVALTVALALGAWFGSRAFDGATARDTAPSSASMSDRPTDGISIATREPEG
                     SLVGTPSDSAADTARVVIGSDKPKRGVQSMDTVQPATPTRRDEKATGIQKDAPLSTTT
                     VASRDAAKSTRTPIEKPAEPRATGENGTKPAISESDAVNGGGEEAKRPARDNPAVAKP
                     ADGTLSVYFLGGVGEFFVNGKRFAQQPPFDKVSLPAGSYRMACRMSGDAAPKEIVVTI
                     RPNQETIIEYELGQDPVVATE"
     gene            6776..7432
                     /locus_tag="VEC56_10675"
     CDS             6776..7432
                     /locus_tag="VEC56_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14655.1"
                     /translation="MKRFLGVAGATLLVFAAVQLVPESSHAQVYQPYWTARPVPDDSP
                     AMGVAMGFGDDMFRIAGHGRFSITSASDLGLELVFDNIENEIGDDDQTFGGGGDFKYL
                     VVGEGERLPFDLAAQAGVGMQWGSDYRLLTVPFGLLGSKSIPVDNGSREITPFAGAYV
                     IVEHATVEVSGADDVSDTDVSAEIRLGGAFQITGRTHAFAAIHAGNGTMFFLGFSAGL
                     "
     gene            complement(7459..8211)
                     /locus_tag="VEC56_10680"
     CDS             complement(7459..8211)
                     /locus_tag="VEC56_10680"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXV14656.1"
                     /translation="MLTLEHASLSDRGRVRQNNEDACAIFVPEGAGELADRGAVFVVA
                     DGMGGHRGGEIASRIAVRTIVAFYTANAEENRSQALARAFREANKTIIEESVADSTLF
                     GMGTTCTALAVNRGRAYIAHVGDSRAYLMRAGRIQQITHDHSIVGEMVRSGILSDEDA
                     RNHPKRNVITKSLGAQDEIAADMPAALELEPGDSFLLCSDGLTAYLSDEDIAAVVSAS
                     PPAEACKKLVKMANDAGGRDNITVLVVTVRSL"
     gene            8458..>9372
                     /locus_tag="VEC56_10685"
     CDS             8458..>9372
                     /locus_tag="VEC56_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14657.1"
                     /translation="MTTTDTPKVRVDETLARYRDLQAQFDRVRDRLRKAEEQKTTVSR
                     RTFDKVRAEYDRELDALRAKMTPLREELESHRSTVEGGLKDANSSLESVEEELAEVVF
                     RHRVGEFSDAELAEKRRSLEARLEDARSRIAEQRRTLDLFDTMREDATPSQPEVVDEP
                     APPMRATERDYDDAPATRSVESRADTAPRPVPRVDPTTDEHARRPVLRPRAGDAHAPS
                     SAPAEPAAAKPAPRSESKPQPSADFENPHDWIKEMGRDTPAKKPAATAPPAPAARPRI
                     SGAPAPSAPTATAVAGKATKRNPARTPSL"
BASE COUNT         1578 a         3186 c         3041 g         1567 t
ORIGIN      
        1 aggacctggg cagcaccaac ggcacctatt tgaacggccg ccgcgtcgcg cgcgagcgcc
       61 tgagccccgg cgacgagctc atcatcgcca acgtcggcat gctggtggag gcgtcgaagt
      121 cgtccatcca cgacgcgcgc ccgccgctca atatcacggt ctcgatcgat cagaagccga
      181 cgaaaccctc ggtgacgatg tcgtccgcgg agatcagcgg gacgcacgag atcctcgaag
      241 gcatcggcgc ggaccccaac gcggcccacc gcgcgctcca ggtgctctac aacgcggact
      301 ccgcgtttcg gaacatcgaa gacctctcgc tgctgctcga caacctgctc gggatcatca
      361 tggaatgcat cccggcctcg cgcggcttcg ttttcctgat ccagcgcgac accaaccagc
      421 tggttccgta cgcgcgccgg ccgcccgggg tggcgtcgga cgacgcggag atcgtggtga
      481 gccagaccat cattcagacc gcggtgcgcc agaaggcgtc gatcatctcg aacgacgccc
      541 tggtcgacga gcgcttcctg cactccaaga gcgtcgccgc tctcgacgtg cgcagcgcca
      601 tgtgcgcgcc cctattcaat cgcagcaacg tgctcggcat cgtatacgtc gactcgcaca
      661 aggcgaattc gttctcgcgc aaggacctcg cgctgttttc cgcactcgcg ctcaaggtcg
      721 cgatcgcggt cgacaacgcg cgcctgtatg acgacctgcg cggattgttc tacaacaccg
      781 tggaaacgct ggtgcgcacc atccaggcca aggaccagta cacggccggt cactcgacgc
      841 gcgtcagccg ttattcgctc atgatcgcgg acaagctggg gctgtcgacc aaggacaagc
      901 accagctcta cctggccgcg atgctccacg acatcggcaa gatcggcgtg cccgacgatc
      961 tcctgaatcg gcctgggcac ctgtcgcagg aggaaatgga gcgggttcgc aaccacgtgg
     1021 tggtgggtgc gtccatgatc gagatgctgg gcgagatgca ccccatcgtg ccgctgatcc
     1081 ggcaccatca cgagtgctac gacgggtcgg gttaccccga cgggttgcag ggagagcaga
     1141 ttccgctgat ttcgcgcatc atcgcggtcg cggacatgta cgacgccatg acctcggacc
     1201 gtccctaccg caagcgccgc acccaccagt tcgccgtgga agagatcctg tcggtgagcg
     1261 gcaccaagat cgaccccaag gtggccgaag cctttctcaa cgtgctcaag gacatcatcc
     1321 cgggcgacga gaagacggaa ccgacctccg aagtgactag cggatctcca actcgctgat
     1381 cgcgacggtc gcgtcgcgtc cctcgaccac gacccgaatc gcgggcgctt ccttgacggc
     1441 cgcgtcgagc ggcacgtccc gaagcgtccc cgtgtccttg aggtccacgt cctggacctt
     1501 cttgtccccc gcgtagatcg cgaccttccc gacgtggcca ccgccagcga cgcgcagact
     1561 caacgtgccc gcttcgagcc cgatcgggag cggaatctcg acgaacgcgc ccttcgcggg
     1621 cgactgccac gcggtggtgg tatcgccgtc gaacgccttc catgcatccg tgcccgacga
     1681 cgcgccgttc atggcgaagc gcgcctcgcg gtcggtcatg agcgtgaatg tctcgccctg
     1741 aatgaagtcg cgcgtggtcc cgcccgccat gaagacgcgc gcgtcccaca ggccgtcgcc
     1801 gttcaactcc acgagctgca acgaatcggg cgcgccgtcc gcgaagtacc aatgccacgt
     1861 gatgccctcg gtggtgttgt tcgcgcgttg catgtacaca accccgccgc gcgcaccgtc
     1921 ctgggaacga taaatggccg cgacggcgcg acgcgccgag agctgcgctg ggacgcgctg
     1981 ggtggtcacc aagcggaagc cccgggcctc gagaaccggc gccagcgtag ccgcctggtc
     2041 cgcggccgcg gttttttgcg tgagctccaa gagcggcgac ttcttggcct cgtctgcggt
     2101 gtcactcgac ttggaacagc cggcaaggag gcagaggcag gccaggaaag cgaaacgcac
     2161 gatactcatg tccgtccttt tcaagcgtat gttgggagtc cacgtgtccg gcggaatccg
     2221 gggcagcgag aaacgtcggg acgaattgtg tgagatcggc cccgaattgt caagggacgg
     2281 cccgcgcggg ggagtttccg aattgggcgg ctaccgcgcc gccgcgaccg gcggcacggt
     2341 cgcgcgatcg ttgtagaggt ggtacatgag gttgcccagg aggtccgcgc cggccttggt
     2401 gaaccgcttg gggctgccac tgtggccgta gtagaacagg cgcttcttgt tcgagaagat
     2461 gtcggggagt tggaagtcga ccagcgccca caggtcgttg ggcaggacga tgaccaatcc
     2521 ggtgcacgcg tgcaggttga cccccgcggg agggctggcc gaatcgatgt cgacgccgat
     2581 cgttcgctcc ggaaccgacg atatatcgac ccactgggcg agtccgccgc tcgttaccgt
     2641 gtggggcgtc gtgagcacac tcgcgctcgg ggtcgtgacg tagtagctgg tctggttgcg
     2701 cggctgcgaa ttcgcgtcgg atatcgaggg aactgcgagc tgcaagtagg ccaggccgcc
     2761 ctctcccagt gccagcacgt tggcggtgct ggccgcgatc gccagcgcgc gcgccggcac
     2821 cccgtcgccc cagtcgccgg tgtcggtacc ggtgtcgtcg gcgacgatca cgatgtcgaa
     2881 tacgctcatg tcgcgccaat cgagatccgc gatgggtacg ggagcgacac cgctgtagcc
     2941 ataatcgctc cagtagccga acaacacttc catgtactcg gccgcccact ccccgctcga
     3001 cgtcacgtag gcgacgcgct gcacgatgag ggcgtcgtcg gaggtgtccg ttccgaggag
     3061 gctggaggcg gagatgtact cggtcgtggc ggcgcgcggc acacgcacat ccagcgatcc
     3121 ccagctggcc gacacgattg tcatttccat cgagccgatc caaacccgcg tgccggggaa
     3181 gtcgaggttt tcgcccagaa tcgtcatcac gtcgcccggg atcgcggccg gcgcgctgaa
     3241 gccggttatc gcgatctgcc ccgatccgcc catcacgtag ccgaattcgg ggatgctcgc
     3301 gagtccctgc gcatagtccg tgcgcggcac cacgcggaaa tagtgcgatc ccgtgggcag
     3361 ttgcacggtg aggaacgtat ccgtcaccga ggcctcccag tcgatagtgc cgaaatcgag
     3421 cgacccgctc gcctgcacgt cgtagcgcga ggccgccggc gcgccggtcc acgccaagtc
     3481 gaacgactcc ggatcgacga ccacgctcgg ggtgaccgac agcgaggcga ccaggaagac
     3541 gtacgcgtcg acggtgacgt cggtccggcg accgaccgcg acgtccacac cggtccggta
     3601 gccatgatgc gtgaagtaac ccgactgata cagttcgacg ctgacgcgct tgccgttctc
     3661 ggcaatgcac gggatcgaca actcgacggg gtcgacgccg acggccgcgg accccgcgac
     3721 ctcttgcgtg accggcgttc ccgggcggaa cacacgcacg accacggagt cgatcaccgt
     3781 ggtcgccgcc gttgcccggg aggcgtcacg cgcgaccgcg gacgcgagcg ccgccggctc
     3841 gaagcgcatt acgatgcggc cctccggcac gagctcgggc tccgtcgctc gtgagcacgc
     3901 gagggcgtgc acgagcgcga gcagcgcgac gatgcgcaga ctccggctca atggacgatc
     3961 acccccacgg tgacgccccc agtctccgcc ggcggcggcg ttccgtcgtc gcccccgccc
     4021 ccagccgcca cacccacggc ggcgatcaac gccgccgcgc cggcataaaa ccaccacttc
     4081 ttgtagatcg gtttgcccgc ttcggccagc gcgtccgatg ggagcgcagc cgcgccccgg
     4141 tcgcgatcgc tcgcgttctt ccccgccgaa gagggcgcat tcttgagcgc gagcgcggcg
     4201 tactccgagc gcgcctgcgc gtacgcatcc atgacttcga gcttgaacac gttgggatcg
     4261 ggccccggat acgaaggctt cttcgcgatc atctccgaga acatttgctt gcccatggcc
     4321 ggggccccgc ccaacacgta cgacgcggcc agcacttcgc gcgcgcgcat cgcctcgggt
     4381 tcggggaggt cgacgcgggc caggtaggtc tgcagcgatt cgatcgcctg ctggtacttg
     4441 ccgcggaagt agtacttgta ctcgatctgc ttcaagtcgt cgccgggcgt ccccgcgacc
     4501 gcggtcgcaa acggcatcgt caccgtcgac gcgaatagcg tcacgatcaa caagccgctc
     4561 gtgattcgtc gtgtgcgatt catctgcatc tactccgtcg caacgaccgg gtcttgcccc
     4621 agctcgtact cgatgatcgt ctcctggttg ggccgaatgg taaccacgat ctctttcggc
     4681 gccgcatccc cactcatccg gcacgccatt cgatacgagc ccgcgggcag ggagaccttg
     4741 tcgaacggag gctgctgcgc gaagcgcttc ccgttgacga agaactctcc gaccccgccc
     4801 aggaagtaca ccgacagcgt tccgtcggcc ggcttcgcga ccgccgggtt gtcccgcgcg
     4861 ggtcgcttcg cctcttctcc gccaccgttc accgcatccg actccgatat ggcaggcttc
     4921 gtgccgttct cgccggtggc gcgtggttcc gccggcttct cgattggggt gcgcgtcgac
     4981 ttcgcggcgt cgcgcgacgc aaccgtggtg gtcgacagcg gcgcgtcctt ctggattcct
     5041 gtcgctttct cgtcgcggcg cgtcggcgtc gccggttgaa ccgtgtccat cgactgcacc
     5101 ccgcgcttcg gcttgtccga gccgatcacg acccgcgcgg tgtcggcggc ggaatcgctc
     5161 ggtgtgccca cgagcgagcc ctcgggctcg cgggtcgcga tcgaaatgcc gtccgtcggt
     5221 cggtccgaca tcgacgccga cgatggcgcg gtgtcgcgcg cagtcgcacc gtcgaaggcc
     5281 cgcgagccga accacgcccc cagggcgagt gcgaccgtca gcgcgacgcc ccccaccccc
     5341 accacccagc gccggcgcga gcgcgcgggg cgtatcgcct tgcccgctcg cacgggtgcc
     5401 acgcgcgacg tggtcgcggc cgatgcggtc gccggagtcg ccgcttcgat cgtcaccgta
     5461 cggctggccc cgccctgctg tgacttgatg cgatcgaggt tcttgcgcgc gcgctcgttg
     5521 ttcgggtcga ggaagagaat gcgtcgaaac tccagggccg cgtcgtccag gtggtgctgt
     5581 cccttctgca ggtagaaagt tccgcgggag aggcaattgg ctacggtctt ttcgagcacg
     5641 gccgcgtcgt acgcgtccgg gttcttgata tacgaaccga gccggcggcg atcgcgcgag
     5701 atcttcgctt tctccatcgc ggcttcgagg tcgctgatga gcgcggtcgc gttcgggtag
     5761 cgatcacggt cgttcttctg gagcatgcgc gagacgatgg ccgcgaaatc gggctcgatg
     5821 actggattga cctggatcac cgacggcggc gcgtaggtct ggatcttctc gagcacgtcg
     5881 gagtaggtct tcccctcgaa gggcttgcga cgcccgaaca gctcgtacgc caccacaccc
     5941 agcgagaaaa tgtcgctggc cgtccccacg ggacggcccg ccgcctgttc gggcgacatg
     6001 tacgcgggcg tcccgatgac cttgcccagc tgcgtgatca tcgtcacgga gtcggacttc
     6061 ttcgcgaggc cgaaatccgc gaccttgatc ccgccctggt cggtgaagat gatattggcc
     6121 ggcttgacgt cgcgatggac gatgtcgagg tggtgggcag cttccagccc gaacgcgacc
     6181 tcgaggatga tttgcgtggc gacgtcgaag ggcacgccgg cgcgctcctt catcaagtcc
     6241 tccagggtgc gcccctggta gtactccatg acgatgaaga acgacccctc gctctcgccg
     6301 aagtcgatca cattgacgat gttttcgtgc tgaatgctgg cggcggcttt tgcctcgcgc
     6361 tggaaacgct ctttgaagct cgcgtcggtg gcgaggtagg ggaagagcac cttaatggcg
     6421 acggtcttgt cgatcgagat cttgcgcgcc ttgtagacgg cggccatgcc gcccacgccg
     6481 atctgctcga gcgagtcgta accgtcgatt tggacgcgta ttgccatttg caagctcttc
     6541 ccgaaagcgg tcgactggga gaccccgcgc ccgcgctatg tcgttttttc ccaattggtt
     6601 aaacgcgaca gacgggggcc gaggcggtat cagcaatttc caagccaccg ggaccgtcgt
     6661 agccccttct cggccatggg ttttcgggtt gtcggcgcga ccgttcggtt gttataaagc
     6721 tcgcacgacg cgcggcattc agccgcgcgc gctaccgaaa cgcggtgcaa acagaatgaa
     6781 acgattcctc ggcgtcgcgg gcgcgacgct cctcgtcttt gcggcggtgc agttggttcc
     6841 cgagtcgtcg cacgcccagg tctaccagcc ttactggacc gcgcgtccgg tccccgacga
     6901 ctcgcccgcg atgggtgtgg cgatgggctt cggcgacgac atgtttcgga tcgcggggca
     6961 cggccgcttc agcatcacgt ccgcgtccga cttgggcctc gagttggtgt tcgacaacat
     7021 cgagaacgag atcggcgacg acgatcagac cttcggcggc gggggagatt tcaagtacct
     7081 ggtggtgggc gaaggcgagc gtctgccgtt cgatttggcg gcgcaggccg gcgtgggcat
     7141 gcaatggggc tccgattatc gcctcctcac ggttcccttc ggactgcttg ggagcaagtc
     7201 gattccggtc gacaacggca gtcgcgagat cacgcccttc gcgggcgcct acgtcatcgt
     7261 cgagcacgcg acggtcgagg tatccggcgc cgacgacgtc tccgatacgg acgtcagcgc
     7321 ggagatccgc ctgggcggcg cgttccagat cacggggcgc acgcacgcct tcgcggccat
     7381 ccacgccggc aacggcacga tgttcttctt gggattttcg gcgggattgt agggacagaa
     7441 ggctgctggg ctcgcggcct acagcgagcg caccgtcacc acgaggaccg tgatgttgtc
     7501 gcgaccgccg gcatcgttcg ccatcttgac cagtttcttg caggcctccg cgggcggcga
     7561 tgccgacacg accgcggcga tatcttcgtc cgagaggtag gccgtcagtc cgtccgagca
     7621 gagcaggaac gaatcgcccg gctccaactc gagtgccgcc ggcatgtcgg ccgcgatctc
     7681 gtcctgcgca cccagcgatt tggtgatcac gttacgcttg gggtgattgc gggcgtcttc
     7741 gtcgctcagg atgcccgagc gcaccatctc gcccacgatc gagtggtcgt gggtgatttg
     7801 ctggatgcgt ccggcgcgca tgagatatgc gcgtgaatca ccgacgtgag cgatgtacgc
     7861 gcgcccccgg ttgaccgcca gcgcggtgca cgtcgtcccc atcccgaaca acgtcgagtc
     7921 ggcgacggat tcctcgatga tggtcttgtt ggcctcgcga aaagcccgcg cgagcgcctg
     7981 cgaccggttc tcttccgcgt tcgccgtata gaaagcgacg atggtgcgga ccgcgatacg
     8041 gctggcgatc tcgccgccgc gatggccgcc catcccgtcg gcgacgacga agacagcccc
     8101 ccggtccgcg agctcccccg ctccctcggg aacgaagatg gcgcacgcgt cctcgttgtt
     8161 ctgccgaaca cgaccgcggt cgcttagaga agcatgttcg agggtcagca taaatcggcg
     8221 ggggccccaa ccacccgttg cgagggatcc agacggccga tgaagactac gaaagacccc
     8281 ttccgtcgtc aacccggaac accccgtcca ttcccccccg atgcttaaag aatgccaaac
     8341 cgggccgatt ccctcccggt tggcacagtt tacgctttaa cagtcgtgat ttggtcgccc
     8401 ccggcagatg cccggcggcc acgaacccgc gagcctcgag tgaaggagcg agaccgcatg
     8461 accacgaccg acaccccgaa agtacgcgtc gacgagaccc tggcgcgcta ccgtgatctg
     8521 caagcgcagt tcgatcgcgt gcgcgatcgt ttgcgcaaag cggaggagca gaagacgacc
     8581 gttagccgtc gcacgttcga caaggtgcgg gccgagtacg accgcgagct ggacgcgctg
     8641 cgcgcgaaga tgacaccgct gcgcgaggag ctcgaatcgc accgctcgac ggtcgagggc
     8701 ggcctcaagg atgccaactc gtcgctcgag tcggtcgagg aagagctggc cgaagtggtg
     8761 tttcgccacc gcgtgggcga gttcagcgac gccgagctcg cggaaaagcg ccgctcgctc
     8821 gaagcgcgtc tcgaagacgc gcgctcgcgc atcgcggagc agcgccgcac gctggacttg
     8881 ttcgacacca tgcgtgaaga tgcgaccccg tcgcagccgg aggtcgtcga tgagccggcc
     8941 ccgccgatgc gcgcgaccga gcgcgactac gacgacgcac ccgcgactcg cagcgtcgag
     9001 tcgcgcgcgg acaccgcacc ccgccccgtc ccgcgcgtgg acccaaccac cgacgagcat
     9061 gcgcgccgcc ccgtcctgcg cccgcgcgcg ggtgacgcgc acgcgccgag cagcgcgccg
     9121 gcggagcccg cggccgccaa accggcgccg cgcagcgaga gcaagccgca gccgagcgcc
     9181 gatttcgaga acccgcacga ttggatcaag gaaatgggcc gcgatacccc ggcgaagaaa
     9241 cccgccgcga ccgcaccgcc cgcaccggcg gcgcgcccgc gcatcagcgg tgccccggcg
     9301 ccatcggcgc cgaccgcgac cgcggtggcc ggaaaggcga ccaagcgcaa tccggcccgc
     9361 acgcccagcc tg
//
LOCUS       DASPGK010000389         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1265767, whole genome shotgun sequence.
ACCESSION   DASPGK010000389 DASPGK010000000
VERSION     DASPGK010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265767"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VEC56_10690"
     CDS             complement(<1..1179)
                     /locus_tag="VEC56_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXV14658.1"
                     /translation="MLKDVLRTKNIARIIETANGHDALRRHLGPIQLTLLGIGAIIGA
                     GIFSTVGTAAAGGASHSGAGPALILSFVLTAITCGFAALCYAEFASMVPISGSAYTYA
                     YATLGEIIAWIIGWDLIIEYAVGNVAVAISWSGYFQELLRVVGLGIPDWLGIDYRSAS
                     QAAEQVKVAMSQGPEALAALGDATIRAGQAFGHAPKILGVPFIMNFPAFAIVGVITTI
                     LVIGIRESAWFNTSMVVLKLAIIGFFVIFGAFYVEPENWRPFAPNGTLGIFSAAAIIF
                     FAYIGFDAVSTASEEAKNPKRDMPIAIIASLVICTIIYIAVAIVLTGMLPWTQLGTAE
                     PLATAFSGRGMTWPTLIIALGAVFATTSVLIVFQLGQPRIFFSMARDGLLPKWAASVH
                     P"
     gene            complement(1334..2995)
                     /locus_tag="VEC56_10695"
     CDS             complement(1334..2995)
                     /locus_tag="VEC56_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HXV14659.1"
                     /translation="MKRLIPTLSIVLAATALASSSHSSGDARTRGLNTITSDDIAAHV
                     RVLASDEYEGRMPATRGEEKTLAYITERFRSFGISPQPNASYLQEVPLVKKSIDAEAT
                     RLVVAGQGKETTLVLGENMTAKTGGAVERVTVARSELVFGGFGIVAEDLGWNDYAGVD
                     VTGKTVVLLIQDPRGAADSLYFKGRALSHYGTGIHKSETAARKGARGVIFIHDQDAIG
                     YPFAAIAANAKRPSFERVRGPESAPIPEFTATISKDLAAEMFASAGQDLATLTEAASR
                     KGFRARPVGLSLSGEIATELEYSTSHNVVGYIPGRDRPDEFVVYTAHWDHVGIGAAVD
                     GDSIYNGAVDNATGTAALLELAQAYRALPDAPSRSVVFIATAAEEQGLLGAYHYADHP
                     VFPLANTIAVINMDALFPFGEFNGMTVVALGSSELEEYMEAAASELGRKLQTDPSPEF
                     GAFFRSDHYPFAKKGVPAIFAVGGPLDTPEPSAEMLERFNTYMSAGYHHPEDEYRDDW
                     DLSGVTGDVKIYFLTGYAIANDTRVPNWYPDSEFRALRDRQREAR"
BASE COUNT          538 a          990 c         1047 g          526 t
ORIGIN      
        1 cgggtgcacc gacgccgccc actttgggag cagcccgtcg cgcgccatcg agaagaaaat
       61 gcgcggctgc ccgagctgga acacgatcag caccgacgtg gtggcgaaca ccgcgcccag
      121 cgcgatgatc agcgtcggcc aggtcatgcc gcgcccggag aatgcggtcg cgagcggctc
      181 cgcggtgccc agctgcgtcc acgggagcat gcccgtgagc acgatcgcca ccgcgatgta
      241 gatgatcgtg cagatcacca ggctcgcaat gatcgcgatc ggcatgtcgc gcttggggtt
      301 cttcgcctct tcggaggcgg tcgagacggc gtcgaatccg atatacgcga agaaaatgat
      361 cgcggcggcg gagaagattc ccagcgttcc gttgggtgca aaaggtcgcc agttctcggg
      421 ctcgacgtag aacgcgccga agatgacgaa gaagccgata atcgcgagct tcagcaccac
      481 catcgaggtg ttgaaccacg cgctttcgcg aatcccgatc accaatatgg tcgtgatcac
      541 gcccacgatc gcgaaggcgg gaaagttcat gatgaacggg acgccgagga tcttgggggc
      601 atgcccgaag gcctggcccg cgcggatggt cgcgtcgccg agtgcggcga gggcttcggg
      661 cccttgcgac atcgcgacct tcacctgctc ggcggcctgc gaggcgcttc gatagtcgat
      721 cccgagccag tccggaatcc ccagccccac cacgcgcagc aactcttgga aataccccga
      781 ccacgagatc gcgaccgcca cgttgccgac cgcgtactcg atgatgagat cccagccgat
      841 gatccacgcg atgatctcgc cgagggtggc gtacgcatag gtgtaagccg agccggagat
      901 gggcaccatc gacgcgaact cggcgtagca cagtgccgcg aagccgcagg tgatcgcggt
      961 gaggacgaag gagaggatga gcgctggacc cgcgcccgag tgcgaggcgc cgcccgcggc
     1021 cgcggtcccc acggtggaga agataccggc accgatgatg gcgccgatgc cgaggagcgt
     1081 cagctggatc gggccgagat gacgccggag ggcgtcgtga ccgttggcgg tctcgatgat
     1141 gcgcgcgatg ttcttggttc gcaggacgtc tttgagcaca aggacctccc gggcaatttc
     1201 gcggagtgta ccgcgcggaa ggccctcgac tgcaacggct ttttgggagc gccggaaagc
     1261 agacgaccgg catcgaagaa gatggtgatg atcaagtgac gaccgccaga cgccgcgcgg
     1321 tcgtcaccga tcgtcatcgc gcctcgcgct gacggtcgcg gagggcgcga aattcgctgt
     1381 cgggatacca attcggcacc cgcgtgtcgt tggcgatggc gtagccggtg aggaagtaga
     1441 tcttcacgtc gccggtcaca ccgctcaggt cccagtcgtc gcggtattcg tcttcggggt
     1501 ggtgatagcc ggccgacatg tacgtgttga aacgctcgag catctcggcg gacggctcgg
     1561 gcgtgtccag cggtccgccg accgcgaaga tcgccgggac tcccttctta gcgaagggat
     1621 agtgatcgga gcgaaagaac gcgccgaact ccgggctcgg atccgtctgt agcttgcggc
     1681 cgagctcgga cgccgcggcc tccatgtact cttccagctc cgagctcccc agcgccacca
     1741 ccgtcattcc gttgaactcg ccgaagggaa acagcgcgtc catgttgatc acggcgatgg
     1801 tgttggcgag cgggaacacg gggtggtccg cgtagtggta cgcacccagg agcccttgct
     1861 cctcggccgc ggtcgcgatg aacaccaccg agcgcgacgg cgcgtcgggc aacgcgcggt
     1921 aggcctgggc cagttcgagt agcgcagccg ttcccgtcgc gttgtcgacg gcaccgttgt
     1981 agatcgaatc cccgtccacg gccgccccaa tgccgacgtg gtcccagtgc gcggtgtaca
     2041 cgacgaactc gtcggggcga tcgcgtccgg ggatataacc caccacgttg tgcgacgtcg
     2101 aatactcgag ctccgtcgcg atttccccgg acaacgaaag ccccaccggt ctcgcgcgga
     2161 atcccttgcg cgacgccgct tccgtcagtg tcgcgaggtc ctgccccgcg ctcgcgaaca
     2221 tctcggcggc gagatccttc gagatggtgg cggtgaactc cggaatgggc gcgctctcgg
     2281 ggccgcgcac gcgctcgaag ctgggacgct tcgcgttggc cgcgatggcg gcgaagggat
     2341 acccgatcgc gtcttgatcg tggataaaga tgacgccgcg cgcccctttc cgggccgccg
     2401 tctcgctctt gtgtatgccg gtaccgtagt gcgagagggc gcgtcccttg aaatagaggc
     2461 tgtccgcggc gcccctggga tcttgaatga gcagcacgac cgtcttgccc gtgacgtcga
     2521 cgcccgcgta gtcgttccac ccgaggtctt ccgcgacgat gccgaaaccg ccgaacacga
     2581 gctcgctgcg cgcgaccgtc acgcgctcga cggcgccgcc cgtcttcgcg gtcatgttct
     2641 cacccagcac gagcgtcgtt tccttgccct ggcctgcgac caccagccgc gtcgcttcgg
     2701 catcgatcga cttcttcacc agcggcactt cctgtagata gctcgcgttg ggttgcggcg
     2761 agatgccgaa agagcgaaag cgctcggtga tgtacgcgag ggttttttct tcgccgcgcg
     2821 tggccggcat gcgtccttcg tactcgtcgg acgcgagcac gcggacgtgc gcggcgatat
     2881 cgtcgctggt gatggtgttg agaccgcgcg tccgcgcgtc tccgctcgag tgggacgacg
     2941 aggccagcgc cgtggcggcg agaacgatcg acagggtcgg gatgagtcgt ttcatggctc
     3001 cagcttacga ccggcggccg gagccggctt tcacgttctt gctcgtttgc tagtggggcc
     3061 gggcgacgag gcggcgaatc gcgctgggcg caagccccat g
//
LOCUS       DASPGK010000390         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_680721, whole genome shotgun sequence.
ACCESSION   DASPGK010000390 DASPGK010000000
VERSION     DASPGK010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680721"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="argC"
                     /locus_tag="VEC56_10700"
     CDS             complement(<1..1017)
                     /gene="argC"
                     /locus_tag="VEC56_10700"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXV14660.1"
                     /translation="MPATVQPAVVGATGYTGAELTRLLIKHPRCRVPLLLHDREDGAP
                     GDFAELYPQCVDATARTGTLTIERFAWDRIREAGVDVLFLCTPHEVSRRLAPEAVERG
                     HRVVDLSGAWRLKNAANRAVYAFEDANPHAASLLDASAAYGIPELNAPDIAGARLVAN
                     AGCYATSVILALAAWVDGGLLDLEHGVIVDAKSGVSGAGKQPTARTHFVEVSDNLSAY
                     SVFGHRHAGEMRERLGLDESQLQFTPHLLPIPRGILSTIYVRAKEGTRAADLDACLRE
                     FYARAPFVRVHGSARLPEIRFALHTNFCDLGFAMEPDGRRVVLVSCLDNLVKGAAGQA
                     VQNMN"
     gene            complement(1017..1457)
                     /gene="argR"
                     /locus_tag="VEC56_10705"
     CDS             complement(1017..1457)
                     /gene="argR"
                     /locus_tag="VEC56_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HXV14661.1"
                     /translation="MSKLSRQKVIQDIVTKKSVASQDELRLHLSRRGHAVTQATLSRD
                     IHELGLVKTPEGYSFLPDDRAKGHLVSVERLVREFVLDAKSAQNLVVVRTSAGSAQGV
                     AAALDAESWPEVLGTVGGDDTILIVSPDARHATLLVRRIREILN"
     gene            1591..2166
                     /locus_tag="VEC56_10710"
     CDS             1591..2166
                     /locus_tag="VEC56_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXV14662.1"
                     /translation="MTKNRLEAFSDGVLAIVITILVLELKIPHGSEFGVLRPLVPVFL
                     TYVLSFVYLAIYWNNHHHMLHTVYRVNGRILWANMHLLFWLSLVPFTTGWMGENHFAS
                     EPTALYGVVLLMAAVAYTILQREIVRAHGPESILAAAVGRDTKGKVSMLAYVAAIALA
                     FVEQWISDGIYIAVALLWLVPDRRIESKLEH"
     gene            complement(2178..3032)
                     /locus_tag="VEC56_10715"
     CDS             complement(2178..3032)
                     /locus_tag="VEC56_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14663.1"
                     /translation="MPKFAFMLAVVLTAVILVPSAGRAQTGDLPAYLEDRGTGQPTSM
                     FGTYIKKGELLVYPFFEYYHDDDAEYTPDELGYGLDMDFRGKYRAEEYLIFLAYGITD
                     WLALEFEMAVYIGAELETSPDDPTAVPGEIEESGTGDVEGQIRARLARETDSRPEFFS
                     YLEIVTPQQKDKVLIGTPDWEYKLGFGAVRGFSFGTMTVRLAAEYSMEESATEFGEYA
                     LEYLNRLSPSWRIYLGIEGSQDEVELIPEAQWHIKTDTIVVKLNSAFGLTSKAADWAP
                     EVGIVFSF"
     gene            3156..3374
                     /locus_tag="VEC56_10720"
     CDS             3156..3374
                     /locus_tag="VEC56_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14664.1"
                     /translation="MRIHSVMLVWAAAAAVCACSKDADPASKAPRALTQAQRDSAIAA
                     SKLPGAGVVGKALEVADSAQARAEDSEP"
     gene            3399..4034
                     /locus_tag="VEC56_10725"
     CDS             3399..4034
                     /locus_tag="VEC56_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXV14665.1"
                     /translation="MRVGILGSGDVGQALAAGFSQHGHAVTIGTRSPAKLAAFASHHK
                     GVRVGSFRDASKAGEIVVLAVKGSAAADALRDADEDNIEGKIVIDTTNPISDAPPKNG
                     VLDYFTAEESLMERLQREFSGARFVKAFSCVGSALMVHPKLAGGPPTMFICGNDADAK
                     KTVTGILDQFGWDVEDMGAVEAARAIEPLAVLWCIPGFLRNDWMHAFKMLR"
     gene            complement(4100..5194)
                     /locus_tag="VEC56_10730"
     CDS             complement(4100..5194)
                     /locus_tag="VEC56_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14666.1"
                     /translation="MKRNRWCMNLLAATLVTVWAFGCDRATDTDPAGDVSSRGQKVDL
                     DDTYGGFSTADEAPGFGDPMLLADYGPDGDVAYLDDVDTTHTDRRHPRRYLMITWGNL
                     RCDSLIDFATDWTGGLCAENGVVRVVRTIRFEPRDYLVPRTSRECVEWVSHTRPHFDG
                     VIVALHKTMDCDSLTDPAVVDTLCDRPLSVTFKTGPLTVTFSEDELADLHRVIPVDDA
                     GNAVAFNVITRAPGECANGFLAGQWKPSPGRNHKGLQGWFRGKWVSENGLHMGYLRGF
                     YGIRAQDEHVFFGKWVSESGRFRGLLKGHYGLFEETDTGAEGWFEGVWFSRQLRSVGG
                     LEGVWGTGDDDKEGGFFRGAWRERCATPAL"
     gene            complement(5289..>5604)
                     /locus_tag="VEC56_10735"
     CDS             complement(5289..>5604)
                     /locus_tag="VEC56_10735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXV14667.1"
                     /translation="DRLLPIMSYPAGVSPYGCFDMAGNAAEWVDGRYAAYERGPDDVV
                     PKGVPDRNELFSKDRHIYRGGSWNTFSKYLRCANRESTPPDKRWVYVGFRCAMDPPWV
                     KK"
BASE COUNT          926 a         1847 c         1895 g          936 t
ORIGIN      
        1 gttcatgttc tgcacggcct ggcccgccgc gcccttgacc aggttgtcga ggcacgagac
       61 cagcaccacg cgacgcccat cgggctccat cgcgaagccg aggtcgcaga aattcgtatg
      121 gagcgcgaag cgaatctccg gcagccgcgc gctcccgtgg acgcgcacaa acggggcgcg
      181 cgcgtagaac tcgcgcaggc acgcgtcgag gtcggccgcg cgcgtgccct ccttcgcgcg
      241 aacgtagatg gtcgaaagga tcccgcgcgg gatcggcaac agatgcggcg tgaactggag
      301 ctggctttca tccaggccga gccgctcgcg catctcgccc gcgtgccgat gcccgaacac
      361 gctgtaggca gacaagttat cgctcacctc gacgaagtgg gtgcgcgccg tgggctgctt
      421 gccggcgccc gagacgcccg acttcgcgtc gacgatgacg ccgtgttcga gatcgagcag
      481 gccgccatcc acccacgccg ccagcgcgag aatgaccgag gtcgcgtagc agccggcgtt
      541 ggcgaccagg cgcgcgccgg cgatgtcggg cgcgttgagt tccggaatcc cgtacgccgc
      601 ggacgcgtcg aggagcgaag ccgcgtgcgg gttcgcgtcc tcgaaggcgt acacggcgcg
      661 gttggcggcg ttcttcagac gccaggcgcc gctcaagtcg accacacggt ggccgcgctc
      721 gaccgcctcg ggtgcgagcc gccgcgatac ctcgtgcggc gtacacaaga acagcacgtc
      781 gacgccggcc tcgcgaatgc gatcccacgc gaaccgctcg atcgtgagag tcccggtgcg
      841 cgcggtggca tccacgcact gtggatagag ctcggcgaaa tcaccgggcg ccccgtcttc
      901 gcggtcgtgc aggagcaacg gcacgcggca acgcggatgc ttgatgagaa gacgggtgag
      961 ctcggcgccg gtatagcccg tggcgccgac caccgcgggc tgcacggtgg cgggcactag
     1021 ttgaggatct cccggatgcg tcggacgagc agggtggcgt gcctcgcgtc gggcgagacg
     1081 atgaggatgg tgtcgtcgcc ccccacggtg ccgaggacct cgggccagct ctccgcgtcg
     1141 agcgcggctg ccacgccctg cgcgctgcct gcgctcgtgc ggaccaccac gaggttctgc
     1201 gccgacttgg cgtcgagcac gaactcgcgc accagccgct cgaccgagac caggtgaccc
     1261 ttggcgcggt cgtcggggag gaacgaatag ccctccggcg tcttgaccag gcccagctcg
     1321 tggatgtccc gcgagagcgt ggcctgggtg acggcgtggc cgcgccgcga gaggtgcagg
     1381 cgcaactcgt cctggctcgc caccgatttc ttggtgacga tgtcctggat gaccttctga
     1441 cgggagagct tcgacatgta taactattcc gtaggtggca ttattatgca tccgccggaa
     1501 acgtcaagaa gaatttcccg gcggtgggtg ctgggcgcga gtgccgcggt cggcggggac
     1561 gtggacggag gaatcgccta tactgcggcc atgaccaaga accgcctcga ggccttcagc
     1621 gacggcgtcc tcgccatcgt catcacgatt ctggtcctgg agctgaagat cccgcacggc
     1681 tccgagttcg gggtgttgcg gccgctggtg ccggtgttcc tcacctacgt gctgagcttc
     1741 gtctatctcg ccatctactg gaacaaccac caccacatgc tccacaccgt ctaccgggtg
     1801 aacggccgca tcctgtgggc gaacatgcac ctcttgtttt ggctgtcgct ggtcccgttc
     1861 acgacgggct ggatggggga gaaccacttc gcctcggagc ccacggcgtt gtacggggtc
     1921 gtgcttctga tggcggcggt cgcgtacacg attctgcagc gggagatcgt gcgggcccac
     1981 ggtcccgagt cgatccttgc tgccgcggtg gggcgcgata ccaagggcaa ggtatcgatg
     2041 ctcgcgtacg tcgccgcgat cgcgctggcc ttcgtcgagc agtggatctc ggacgggatc
     2101 tacatcgcgg tggcactcct gtggctcgtc ccggacaggc ggatcgagtc caagctcgag
     2161 cactgacggc ggcgtcgtca gaacgaaaag acgatcccga cctcgggcgc ccagtcggcg
     2221 gccttcgacg tgaggccgaa cgcgctgttg agcttcacca cgatcgtgtc ggtcttgatg
     2281 tgccactgcg cctcggggat cagctccacc tcgtcctggc tcccctcgat cccgagatag
     2341 attcgccacg acggcgagag ccggttcagg tactcgagcg cgtactcgcc gaactcggtc
     2401 gcggactctt ccatcgaata ctcggccgcc aggcgcaccg tcatcgtgcc gaaggagaag
     2461 cctcgtacgg cgccgaaccc gagcttgtac tcccagtcgg gtgtcccgat gagaaccttg
     2521 tctttttgtt ggggcgtgac gatctcgagg taactgaaga actccgggcg cgaatcggtc
     2581 tcgcgggcca gtcgggcgcg gatctgtccc tcgacgtctc ccgtccccga ctcttcgatc
     2641 tcgccgggga ccgccgtggg atcgtcgggc gacgtctcca actcggcccc gatgtacacc
     2701 gccatttcga actcgagcgc gagccagtcg gtgatgccat acgcgaggaa gatgaggtat
     2761 tcctccgcgc ggtacttgcc ccgaaagtcc atgtcgaggc cgtagcccag ctcgtcgggg
     2821 gtgtattcgg catcgtcgtc gtggtagtac tcgaagaacg ggtacaccag cagctcaccc
     2881 ttcttgatgt acgtgccgaa catcgacgtc ggctgaccgg ttccgcggtc ctcgagatac
     2941 gcggggaggt cgccggtttg ggcgcgaccg gcgctgggaa ccaggattac ggcggtgaga
     3001 acgaccgcca gcatgaacgc gaacttcggc atgggcgtgg ctcctttgga acgaggtgtc
     3061 gtccagggaa cccggcagcg ccgtcgattg tgacggcatc gacgcccgcc gctcgtgtac
     3121 catgggcggg cccggtgcag ggcgaaggag gagacgtgcg gatccattcc gtgatgctgg
     3181 tatgggcggc cgcggcggcc gtgtgcgcgt gctcgaagga cgccgacccg gcgtcgaagg
     3241 cgccgcgcgc gctgacgcaa gcgcagcgcg acagcgccat cgcggcctcg aaactcccgg
     3301 gcgccggcgt ggtcggcaag gcgctcgaag ttgccgactc ggcgcaggcg cgcgccgaag
     3361 attcggagcc gtagctcaca acccaagagg agtcggtcat gagagtcggt attctgggat
     3421 cgggcgacgt cgggcaggcg ctcgccgccg gtttttcaca gcacggtcac gccgtgacga
     3481 tcggcacacg cagccccgcc aagctcgcgg cgtttgcgtc gcaccacaag ggcgtgcgcg
     3541 tcgggagctt tcgggatgcg tcgaaggccg gagagatcgt cgtgctcgcg gtcaaaggat
     3601 ccgcggccgc ggatgccctg cgcgacgccg acgaggacaa catcgagggc aagatcgtca
     3661 tcgacaccac caaccccatc tccgacgcgc cgcccaagaa cggggtgctc gactacttca
     3721 ccgccgaaga gagcctgatg gagcgcttgc agcgcgagtt ctcgggcgcg cgcttcgtca
     3781 aggcgttcag ctgcgtgggg agcgcgctca tggtgcaccc gaagctcgcc ggcggcccgc
     3841 ccacgatgtt catctgcgga aacgatgccg acgcgaaaaa gaccgtcacc ggaatcctcg
     3901 atcaattcgg gtgggacgtc gaggacatgg gagcggtcga agcggcgcgc gcaatcgaac
     3961 cgctcgcggt gctctggtgc atcccggggt tccttcgcaa cgactggatg cacgccttca
     4021 agatgctgcg ctgaacgcga gacgcccccg cggtcgtgcg accgcgaggg cgtctcctgg
     4081 ggagcatgcg tggccggatc tagagcgccg gtgtcgcgca ccgctcgcgc cacgcgccgc
     4141 ggaagaaccc gccttccttg tcgtcgtcac ccgtccccca cacgccttcc aatccgccca
     4201 cggaccgaag ctggcgcgag aaccacacgc cctcgaacca gccttcggcg ccggtatcgg
     4261 tttcttcgaa caggccgtaa tggcccttga gcaggccgcg gaatcgtccc gactccgaga
     4321 cccatttgcc gaagaacacg tgctcgtcct gcgctcggat gccgtagaac ccgcgcagat
     4381 agcccatgtg gagaccgttc tcgctgaccc acttgccgcg gaaccacccc tgcagtccct
     4441 tgtggttccg gcccggcgaa ggcttccact gaccggccag gaatccgttg gcgcactcgc
     4501 ccggcgcgcg tgtgatcacg ttgaatgcga ccgcgtttcc ggcgtcgtcg acggggatga
     4561 cgcggtgcag atccgcgagc tcgtcctcgg agaacgtcac cgtcagcgga cccgtcttga
     4621 acgtgaccga caacggccgg tcgcacagcg tgtcgacgac ggccgggtcg gtcagcgagt
     4681 cgcaatccat cgtcttgtgg agcgccacga ttacgccgtc gaagtgcggc cgggtgtgcg
     4741 acacccactc gacgcactcg cgcgaagtgc gcggtacgag gtaatcgcgc ggctcgaagc
     4801 ggatcgttcg cacgacgcgc accacaccgt tctcggcgca taggccgccg gtccagtcgg
     4861 tcgcgaagtc gatgagggaa tcgcagcgca ggttccccca cgtgatcatg aggtagcggc
     4921 gcggatgacg acggtcggtg tgcgtggtgt cgacgtcgtc gaggtacgcg acgtcgccgt
     4981 ccgggccgta gtcggcgagc agcatggggt ccccgaagcc gggggcttcg tcggcggtgc
     5041 tgaagccgcc gtaggtatcg tccaggtcga ccttttgccc ccggctggag acgtcgccgg
     5101 ccgggtcggt atcggttgcc ctgtcacagc cgaacgccca caccgtcacg agggtggccg
     5161 cgaggaggtt catgcaccat cggttgcgtt tcacgagaac cactcctttt tcgttgtgcc
     5221 gacgcagcgg cgtgatgccg caaagcgtcg ctcgggggca ggttcaatgc cgtaccggga
     5281 cggcgcgatc acttcttgac ccacggcgga tccatcgcgc agcggaagcc gacgtagacc
     5341 cagcgcttgt ccgggggcgt gctctcccgg ttcgcgcagc gaaggtactt ggagaacgtg
     5401 ttccacgaac cgccccggta gatgtgacgg tccttactga acagctcgtt tcggtccggt
     5461 acccccttgg gaacgacgtc gtcggggcca cgctcgtacg cagcataccg gccgtctacc
     5521 cattccgccg cgttccccgc catgtcgaag cacccgtacg ggctgacgcc ggcggggtag
     5581 ctcatgatcg gtagcaagcg gtcc
//
LOCUS       DASPGK010000391         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1735781, whole genome shotgun sequence.
ACCESSION   DASPGK010000391 DASPGK010000000
VERSION     DASPGK010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735781"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VEC56_10740"
     CDS             complement(<1..975)
                     /locus_tag="VEC56_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14668.1"
                     /translation="MKVMHVIRREYVENVRRKSFVISTMLVPLLMAAFFVLPVVFALF
                     VPDRQYRVVMVDQVGGVGAEVAAALADTLKSGERKYAVESIDAPGERFDGEREARIAS
                     VQSGEVDIVVAIPATVLSDGKAEYVTREARNFNIFERFTDVVSDAVVARRLEAEGFDF
                     ARVKGLTEPVEMEMSRVSATGEVEEKNFLAEYGLVFIFVMILYMAIFSWGVTISRSIV
                     EEKGSRVIEVLLSSLTPRDLMLGKLVGVGLAGMTQLSIWALVGLAMSGYAATSAVAML
                     SSLEIPPAVFGYLMLYFLLGFLLYSSLFMTVGAACSTEQDAQQFQGLIT"
     gene            complement(976..1878)
                     /locus_tag="VEC56_10745"
     CDS             complement(976..1878)
                     /locus_tag="VEC56_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV14669.1"
                     /translation="MSQSAVEARDVVKRYGDFVAVDRVSITVPQGTIFGLLGPNGAGK
                     STTIRMLLRIMLPDEGAIRVLGNDVAALDVDRIGYLPEERGLYKKMKVGDLLVFFAEI
                     KGVARAEARRRAIQWLDRMGLLDRAGRPIEELSKGMQQKVQFIATLIHEPELLVLDEP
                     FSGLDPVNANVLKDIILDFHRRGHTIVFSTHMMDQVEKLCEHICLINKGRVVLSGTLA
                     SVKKQYGHNGVSVRFNGDGAFLKSLPEVASFQDNGNELFLRLRDGANAARVLDAARGR
                     VEILRFEVAEPSVHDIFIERVSEA"
     gene            complement(1875..2561)
                     /locus_tag="VEC56_10750"
     CDS             complement(1875..2561)
                     /locus_tag="VEC56_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HXV14670.1"
                     /translation="MKIVLATRNRDKAREIVAMLDRLDVEVVTMDAFPAAPETIEDGD
                     TLEANAWKKAREALAHTGVSALADDTGLEVEALGGAPGVFAARYAGENATYADNCRKL
                     IAALEGVPPARRGARFRTVMALALAPADLARLAGAVPRSAEVAKNFSLDTRGLVSEGA
                     LNGAIAVHARGTSGFGYDPVFVDGNSGRTLAEMTLEEKNAASHRYRALLEMREMLLRY
                     GLAREKEPAR"
     gene            complement(2566..3270)
                     /gene="rph"
                     /locus_tag="VEC56_10755"
     CDS             complement(2566..3270)
                     /gene="rph"
                     /locus_tag="VEC56_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXV14671.1"
                     /translation="MRKDGRGAGDLRPVTIERGYLRHAEGSALISVGETRVICSATLE
                     PGVPRWMAGGGRGWITAEYGMLPRSSRQRITREADRGRTGRTHEIQRLIGRSCRAAVA
                     MEVLGENTIIVDCDVLEADGGTRTASITGACVALYDAISTLELREHPMNFFFSAVSVG
                     VVDGVPMLDLAYQEDSSADVDLNVVMAESGAFIEVQGTAERVPFTGEQFDRMLALARD
                     GCARLLRAQREVLGLP"
     gene            complement(3286..4083)
                     /gene="murI"
                     /locus_tag="VEC56_10760"
     CDS             complement(3286..4083)
                     /gene="murI"
                     /locus_tag="VEC56_10760"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXV14672.1"
                     /translation="MSNRQPIGVFDSGIGGLTVLKELLGRLPDESFVYFGDTARVPYG
                     TKSADAVKRFTRESVRLLLDRGVKMVVIACNTASSEVLPEFETEFPVPIVGVIEPGVR
                     AAVAATKQRRIGVIGTPGTIRSGAYQSKIMARLPDAVVIPQACPLFVPLVEEGWIDTP
                     VTRLVAREYLRVYENAQIDALVLGCTHYPLLKPVIADTVGEGVELVDSAIETAREVSR
                     VLADRGIEGRGGGEFHIVLSDTAPSFGDIATRFLGRRVPDIELVSVV"
     gene            complement(4121..4744)
                     /locus_tag="VEC56_10765"
     CDS             complement(4121..4744)
                     /locus_tag="VEC56_10765"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXV14673.1"
                     /translation="MDERPQHPSHDPADERAAARGSLGGRHLAAIAGVALGILFVGMW
                     FLLRTPREPADDARAPAPARTLEGSRAVTLYFAGADEPMIYTETREVGVGRRFDEQVR
                     QVIDALIAGPDEERGVSAIPAGTQLLAVSFDPDAAALYLDFSAELVAAHPGGSAAEYC
                     TVAVIVRTVGENFPEVRAVQLLVDGSQVDTIAGHIRADEPFLVRDWR"
     gene            complement(4760..>5342)
                     /locus_tag="VEC56_10770"
     CDS             complement(4760..>5342)
                     /locus_tag="VEC56_10770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXV14674.1"
                     /translation="NLAIARVVRDLIQAELGLEVVMTRDDDRTVDLVERTEIANTSGC
                     DLFVSIHCNSWFHRGTSGFESYFLSPARSESEKALQRFENEAGDGAAQTPRGDVEFIL
                     WDLVQNAYIAESSTFAEYVQREMTSRLGIPSRGVKQANFIVLQGAKMPAVLIETAFLS
                     NPTEERLLLDPGFQRRVAEGVVASIRKMQDRYR"
BASE COUNT          891 a         1864 c         1706 g          881 t
ORIGIN      
        1 cgtgatcaag ccctggaact gctgcgcgtc ctgttcggtg ctgcacgccg cccccacggt
       61 catgaagagc gacgagtaca acaggaagcc gagcaagaaa tacagcatga ggtacccgaa
      121 cacggccggt ggaatctcga gcgacgacaa catggccacg gcactggtcg cagcgtagcc
      181 gctcatcgcg aggccgacca gcgcccagat cgagagctgc gtcataccgg ccaggcccac
      241 gcccaccagt ttgcccagca tgaggtcgcg cggggtgagc gagctcaaga gtacctcgat
      301 cacgcgcgag cccttctcct cgacgatgct gcgcgagatg gtgacacccc acgagaagat
      361 cgccatgtag aggatcatca cgaagatgaa caccagaccg tattcggcga gaaagttctt
      421 ctcctcgacc tcgccggtgg cactgacgcg gctcatttcc atctcgaccg gctcggtgag
      481 tcccttgacg cgcgcgaagt cgaagccctc cgcctccagg cggcgcgcca ccacggcgtc
      541 gctcacgacg tcggtgaagc gctcgaagat gttgaaattc ctcgcctcgc gggtgacgta
      601 ctcggccttg ccgtcggaga gcaccgtggc cggaatcgcg accacgatat cgacctcccc
      661 gctctgcacc gacgcgatgc gggcctcccg ctcgccgtcg aagcgctcgc cgggtgcgtc
      721 gatcgattcg accgcgtact tgcgttcgcc gctcttcagc gtgtcggcga gcgcggccgc
      781 gacctccgca cccacgcccc cgacctggtc gaccatgacg acacgatact ggcggtcggg
      841 cacgaagagc gcgaacacca ccggcagcac gaagaacgcc gccatcagca acggcaccag
      901 catggtactg atcacgaagc tcttgcggcg cacgttctcc acgtactcgc ggcgaatgac
      961 gtgcatgacc ttcatctatg cctccgacac gcgctcgatg aagatgtcgt ggaccgacgg
     1021 ctcggctact tcgaagcgca ggatctcgac ccgcccgcgg gccgcgtcga ggacgcgcgc
     1081 ggcattggcc ccgtcgcgca ggcgcaggaa cagctcgttg ccgttgtcct ggaagctcgc
     1141 cacctccggc agcgacttca agaacgcgcc gtcgccgttg aaacgcacgc tgacgccgtt
     1201 gtgcccgtac tgcttcttca ccgacgcgag ggttcccgac agcaccacgc gccccttgtt
     1261 gatcaggcaa atgtgttcgc acagcttctc gacctgatcc atcatgtggg tcgaaaacac
     1321 gatggtgtga ccgcggcggt gaaaatcgag gatgatgtcc ttgagcacgt tggcgttgac
     1381 ggggtccagg ccggagaacg gctcgtcgag caccaggagc tccggctcgt ggatcagcgt
     1441 cgcgatgaac tggaccttct gctgcatccc cttcgacagc tcttcgatcg gtctgcccgc
     1501 gcggtccaag agccccatgc ggtccagcca ctggatcgcg cggcggcggg cttcggcgcg
     1561 tgcgacaccc ttgatctcgg cgaagaacac cagcaggtct ccgaccttca tcttcttgta
     1621 gagcccgcgt tcctcgggaa ggtagccgat gcggtcgacg tcgagcgccg ccacgtcgtt
     1681 gcccagcacg cggatggcgc cttcgtcggg cagcatgatg cgcagcagca tgcggatggt
     1741 ggtcgacttg ccggcgccgt tcgggcccaa gaggccgaag atggtgccct gcggcaccgt
     1801 gatcgacacc cgatccacgg ccacgaagtc gccatagcgc ttgacgacgt cgcgggcttc
     1861 gacggcggac tggctcatcg tgccggctcc ttttcgcgcg ccagtccgta gcgcagcagc
     1921 atctcacgca tctcgaggag cgcgcggtac cgatggctgg cggcattctt ctcctcgagc
     1981 gtcatctccg cgagcgtgcg cccgctgttg ccgtcgacga aaacggggtc gtacccgaag
     2041 ccgctcgtgc cgcgcgcgtg caccgcgatc gcgccgttga gcgcgccctc ggagaccagt
     2101 ccgcgcgtat cgagcgagaa gtttttcgcc acctccgcgc tgcgcggaac cgcgcccgcg
     2161 agccgcgcga gatcggcggg cgcgagagcc aacgccatca cggtacgaaa acgagcgccg
     2221 cggcgcgcgg gcggcactcc ctccagcgcc gcgatcagct tgcggcagtt gtcggcgtag
     2281 gtcgcgttct cccccgcgta gcgcgcggcg aacactccgg gcgcgccgcc cagcgcctcc
     2341 acctcgaggc cggtgtcgtc ggccagcgcc gacacgcccg tgtgcgcgag cgcctcgcgc
     2401 gctttcttcc acgcgttcgc ctccaatgta tctccgtctt cgatggtctc gggcgcggcc
     2461 ggaaacgcgt ccatggtaac cacctcgacg tcgaggcggt ccagcatggc gacgatctcg
     2521 cgcgccttgt cgcgattgcg cgtggcgagc acgatcttca tgggatcacg gaaggccgag
     2581 cacttcgcgc tgcgcccgga gcaggcgagc gcacccgtcg cgggcgagcg cgagcatgcg
     2641 atcgaattgt tcgccggtga aaggaacgcg ttccgcggtg ccttgcactt cgatgaaggc
     2701 gccgctctcc gccatcacca cgttcaaatc gacgtcggcg ctggagtcct cttggtaggc
     2761 gaggtccagc atggggactc cgtcgaccac gcccacgctg accgcggaga agaagaaatt
     2821 catggggtgc tcgcgcagct cgagggtgga gatggcgtcg tagagggcca cgcacgcgcc
     2881 cgtgatcgac gcggtgcgcg tgccgccgtc ggcttcgagc acgtcgcaat cgacgatgat
     2941 ggtgttctcc cccagcacct ccatcgccac cgcggcccgg cagctgcgcc cgatcaggcg
     3001 ctggatctcg tgcgtgcgcc cggtacggcc gcgatcggct tcgcgcgtga tgcgctggcg
     3061 cgagctgcgc gggagcatcc cgtattcggc cgtgatccag ccgcgaccac cgcccgccat
     3121 ccagcgcggc acgccgggtt ccagcgtggc gctgcagatg acgcgcgtct cgcccaccga
     3181 gatcaatgcg gagccctccg cgtggcgaag gtagccgcgc tcgatggtca ccgggcgaag
     3241 atcgcccgcg cctcgtccgt cttttcgcat ggttgatgtc tcccgctata cgaccgaaac
     3301 cagctcgatg tccggcacgc gacgccccag gaagcgcgtc gcgatgtcgc cgaagctggg
     3361 ggcggtgtcg ctcaacacga tgtggaactc gccgccgccg cgtccttcga tgccgcgatc
     3421 ggccagcacg cgcgagactt cgcgcgcggt ttcgatggcc gagtcgacca gctccacgcc
     3481 ttcaccgacc gtatccgcga tcaccggttt cagcagtgga tagtgcgtgc accccagcac
     3541 cagtgcgtcg atctgcgcgt tctcgtacac gcgcaggtac tcgcgcgcga ccaggcgcgt
     3601 caccggcgtg tcgatccagc cctcttcgac cagtggaacg aagagagggc acgcctgcgg
     3661 aatcacgacc gcgtcgggca agcgcgccat gatcttcgat tgatatgcgc ccgagcgaat
     3721 cgtccccggc gttccgatga cgccgatgcg ccgctgcttg gtggccgcga ccgccgcgcg
     3781 cacgcccggc tcgatcacgc ccacgatagg caccggaaac tcggtctcga attccggcag
     3841 cacctcggac gacgccgtgt tgcacgcgat gaccaccatc ttgacgccgc ggtcgaggag
     3901 cagccgcacg ctctcgcgcg tgaaacgctt gaccgcgtcc gcgctcttgg taccgtaggg
     3961 gacgcgcgcg gtgtcgccga agtagacgaa cgactcgtcg gggagccggc cgaggagctc
     4021 cttgagcacg gtcagccccc cgatgccgga gtcgaaaaca ccgatgggtt gtcgattgct
     4081 catgtgtgtt ttctcacgcg cgcgcgctca tcgataacgt ctagcgccaa tcacggacca
     4141 aaaacggttc gtcggcccgt atgtgccccg cgatggtatc gacctgggat ccgtcgacca
     4201 acagctggac cgcgcgaacc tcggggaagt tttcgcccac cgtccgcacg atgacggcga
     4261 ccgtgcagta ctccgccgca ctcccgcccg ggtgcgccgc caccaattcg gcgctgaaat
     4321 cgagatacag cgcggcggcg tcggggtcga acgacaccgc caggagctgc gtcccggcgg
     4381 gtatggcact gacgccgcgt tcctcgtcgg gccccgcgat gagcgcgtcg atgacttgcc
     4441 gcacttgctc gtcgaagcgg cgacccacgc cgacttcgcg cgtctcggtg tagatcatgg
     4501 gttcgtccgc gccggcgaag tacagggtca ccgcgcggct tccttccaac gtgcgggccg
     4561 gagcgggcgc ccgcgcatcg tctgccggct cgcgcggcgt tcgcagcaag aaccacattc
     4621 ccacgaacaa aatgcccagc gcaaccccgg cgatggccgc caagtggcgc ccgccgagcg
     4681 acccgcgcgc ggccgcgcgc tcgtcggccg ggtcgtggga tgggtgctgc gggcgctcgt
     4741 ccatagtacc agcctggggc tagcggtagc gatcctgcat ttttcgaatc gacgccacca
     4801 cgccctcggc cacgcggcgc tggaagcccg ggtcgagcag gagtcgttcc tcggtcgggt
     4861 tggacagaaa cgccgtctcg atcagaacgg ccggcatctt cgcgccctgc agcacgatga
     4921 agttcgcctg tttgaccccg cgactgggta tgccgagccg ggaggtcatt tcgcgctgga
     4981 cgtactcggc gaaggtactg ctctccgcga tataggcgtt ctgcaccaag tcccacagaa
     5041 tgaactcgac gtcgccgcgg ggcgtctgcg cggcgccgtc ccccgcctcg ttttcgaatc
     5101 gctgcaacgc cttttccgac tcgctgcgcg cgggtgagag aaagtacgac tcgaacccgc
     5161 tggttccgcg gtggaaccaa ctgttgcagt gaatgctcac gaacaggtcg cagcccgacg
     5221 tgttggcgat ctcggtgcgc tccaccaggt cgacggtgcg gtcgtcgtca cgcgtcatca
     5281 ccacttccag cccgagctcc gcttggatca agtcgcgcac caccctcgcg atcgccaggt
     5341 tg
//
LOCUS       DASPGK010000392         6604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_681186, whole genome shotgun sequence.
ACCESSION   DASPGK010000392 DASPGK010000000
VERSION     DASPGK010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681186"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VEC56_10775"
     CDS             <1..114
                     /locus_tag="VEC56_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXV14675.1"
                     /translation="NRCGRSRAYLRRFGICRICFRDLALMGEVPGVVKASW"
     gene            255..647
                     /gene="rpsH"
                     /locus_tag="VEC56_10780"
     CDS             255..647
                     /gene="rpsH"
                     /locus_tag="VEC56_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXV14676.1"
                     /translation="MTDPIADYLARLRNAAGAKHKYVDVPASRVKAQMSKLLLDAGYL
                     SGVKYIEDNRQGVLRVYLKYTRDKESVLQGSRRISRASRRVYSPKKNLPRVLGGYGIA
                     IVSTSQGILTDVECRKRGIGGEVMCEIW"
     gene            661..1206
                     /gene="rplF"
                     /locus_tag="VEC56_10785"
     CDS             661..1206
                     /gene="rplF"
                     /locus_tag="VEC56_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXV14677.1"
                     /translation="MSRIGKKPVTIPSGVTVSVEKTKVTIKGPKGALTLAVPATCAVA
                     QQGSEVVVTRANEEKQTRASHGTTRAHIANMMRGVTEGFSKDLEIVGVGYKAEAKGKS
                     LVLTIGYSHLVDFKIPDGITVETPEPTKIKVSGIDRQRVGQVAAEIRAVRGPEPYKGK
                     GIKYVDEHIERKAGKSAASGG"
     gene            1233..1718
                     /locus_tag="VEC56_10790"
     CDS             1233..1718
                     /locus_tag="VEC56_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXV14678.1"
                     /translation="MQKKFKTIEDYKRWRRHKRVRNRVVGTMERPRVCVFRSNQHIYA
                     QLIDDETGRTLLAVSSLKMEAPAAAKKAEAKAEKADAKAEGKKGGKAAKADKPAKGEK
                     AAAPAGGKKTALAREVGRLLAEAAQAKGFKKVAFDRGGYLYHGRVAALATSAREHGLD
                     F"
     gene            1755..2462
                     /gene="rpsE"
                     /locus_tag="VEC56_10795"
     CDS             1755..2462
                     /gene="rpsE"
                     /locus_tag="VEC56_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXV14679.1"
                     /translation="METGTNERTPTTPTTPGSPATPDRPARPGGGGGRGGDRGRGPRG
                     GGGGQGRGKDDRGRRGGPRERERERGGPEYIETTVYVNRVAKVVKGGRRFSFNALVCV
                     GDGMGRVGIGLGKSNEISDAIRKAGENAKRSMIRVPLMGGTIPYQVYGKFGAGKVILK
                     PATPGTGVIAGGAVRAIMESAGVRDVLAKSLGSTNPHNLIKATMTALQQLESAKDVAK
                     RRGISINQLFGIEAKNG"
     gene            2455..2640
                     /gene="rpmD"
                     /locus_tag="VEC56_10800"
     CDS             2455..2640
                     /gene="rpmD"
                     /locus_tag="VEC56_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018917722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXV14680.1"
                     /translation="MAKKIRITQIKSVIGVPPLHKKTMTALGFRRTYQTVEKADTPQV
                     RGMIHQVRYLVRVEEGK"
     gene            2643..3086
                     /gene="rplO"
                     /locus_tag="VEC56_10805"
     CDS             2643..3086
                     /gene="rplO"
                     /locus_tag="VEC56_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXV14681.1"
                     /translation="MRLGELKPAAGSKKKRKRIGCGPGSGHGKTSTRGHKGTQSRTGN
                     TKNAWYEGGQMPLQRRLPKRGFTNIFRIGYQVINLGDLDRFDANATVDVDALLEKGLI
                     RHLNNPVKLLADGELKKPLTIKVHAASKKAKEAVEKAGGQVNLVG"
     gene            3119..4432
                     /gene="secY"
                     /locus_tag="VEC56_10810"
     CDS             3119..4432
                     /gene="secY"
                     /locus_tag="VEC56_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXV14682.1"
                     /translation="MFTKIQEIFSIPELKRRVIFTLGLLIVYRIGSHITTPGVDASVL
                     TEFFARQRGTILGLYDLFAGGNLEKATIFALGIMPYISASIILQLFQAVIPYFEKLAK
                     EGEEGRKKITQYTRYGTVVLSIVQGFGIAIFLESMTGPSGTPAVMFSGWGFRILTVIT
                     LTAGTVFVMWLGEQITERGIGNGISLIITVGILARFPIDALNMFRAIQLGQMTPFKAV
                     ILGLMMVLIIAGVIFVTQGQRRIPVQYAKRVVGRRVYGGQASHIPLSVNIAGVIPIIF
                     AQSLIMFPGTIAALFPNVTILQSITLWLSPGHWIYIVLYSLIIIFFTYFYTAIVLNPV
                     DLADNMKKSGGFIPGIRPGKRTAEYIDRILTRITLPGAFFLAFIAVLPDLLITKANVP
                     FYFGGTGLLIVVGVMLDTLRQVESHLLMRHYEGFVKHGRLKGRRG"
     gene            4433..5095
                     /locus_tag="VEC56_10815"
     CDS             4433..5095
                     /locus_tag="VEC56_10815"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXV14683.1"
                     /translation="MNLVLFGPPGSGKGTQAERLRNRWKLRHISTGDLLREAVAAKSE
                     LGKKVESVLASGQLVSDDIILGLMRGAILAVKQDAALSGWLLDGFPRTVGQAKGLDAL
                     LAETGSRIDAIVVLDATREAVVDRLSGRRTCTKCKTVYHITHNPTRVDGVCDKCGGKV
                     VQREDDRPETIAKRLDVYEAQTRPILAHYAGRVATHVIDGDLPVDDVTAEIERIVGRA
                     AA"
     gene            5092..5835
                     /gene="map"
                     /locus_tag="VEC56_10820"
     CDS             5092..5835
                     /gene="map"
                     /locus_tag="VEC56_10820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXV14684.1"
                     /translation="MIMLKTLDEIDIMEANGAIVVECFALARDFIKPGRTRKELDAAI
                     EKLIVERGGKPAFKGFHGYPASTCISVNDEVVHGIPDDRAFQEGDLVGLDIGVFKDGY
                     YVDSARTIPVGRVGKDAEKLLEATWDALAKGIEQARPGNRLTDISHAVEARARKSGFA
                     VVRSLVGHGIGKRMHEDPQVPNFGPPHQGPVLEEGMVLAIEPMVNVGTADVFTLEDGW
                     TIVTADRKLSAHFEDTVAVTKNGPRILTR"
     gene            5850..6068
                     /gene="infA"
                     /locus_tag="VEC56_10825"
     CDS             5850..6068
                     /gene="infA"
                     /locus_tag="VEC56_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXV14685.1"
                     /translation="MPKDDSITVEGTVVEALPNTMFRVELANKHIVLAHVSGKMRMHF
                     IKILPGDRVTLEISAYDPTKGRIVYRYK"
     gene            6078..6191
                     /gene="rpmJ"
                     /locus_tag="VEC56_10830"
     CDS             6078..6191
                     /gene="rpmJ"
                     /locus_tag="VEC56_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXV14686.1"
                     /translation="MKVRTSIKKICPDCKLIRRKGVLRVICKHKRHKQRQG"
     gene            6219..6599
                     /gene="rpsM"
                     /locus_tag="VEC56_10835"
     CDS             6219..6599
                     /gene="rpsM"
                     /locus_tag="VEC56_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXV14687.1"
                     /translation="MRIAGVDIPNEKRIVIALTYIYGIGPKSAETICKKAGISPETRV
                     KSIGEDQGIKLRGIIENDFKVEGELRTELSFNIKRLIDINCYRGRRHRLNLPVRGQRT
                     RTNARTRKGPKVRPGVGKKKAPAK"
BASE COUNT         1332 a         1980 c         2224 g         1068 t
ORIGIN      
        1 aaccgctgcg gtcgttcgcg tgcgtacctg cgccgcttcg gcatctgccg catttgcttc
       61 cgcgacctcg ccttgatggg cgaagtcccg ggagtcgtca aggccagctg gtaagttcaa
      121 cgggcggatc gcttcaagtg ctctccgctc aggtcctcgc gcgagaacgc aatatcgtgt
      181 tgcatcgcgc tgcggaaatc gcgtcgagca tcttgaagcg atccgcctac ggtaggagat
      241 aagagacaat ggcgatgaca gatccgattg ctgattacct ggcgcggctg cgtaacgcgg
      301 cgggcgccaa gcacaagtac gtcgacgtac ccgcatcgcg cgtgaaggcg cagatgagca
      361 agctgttgct cgatgccggg tacttgagcg gcgtgaagta catcgaggac aaccggcagg
      421 gcgtgctgcg cgtctacctc aagtacacga gggacaagga gagtgtgctg cagggttcgc
      481 gccgcatcag ccgcgccagc cgccgcgtgt actcgcccaa gaagaacctg ccgcgcgtgc
      541 tgggtggcta cggtatcgcc atcgtgtcca cgtcgcaggg catcctgacc gacgtcgagt
      601 gccgcaagcg cggcatcggc ggcgaagtca tgtgtgagat ttggtaggga cggtttagac
      661 atgtcgcgaa tcggaaagaa gcccgtgacg atcccatccg gtgtcacggt cagcgtcgag
      721 aagaccaagg tgacgatcaa gggccccaag ggcgcgctca cgcttgccgt cccggccacg
      781 tgcgcggtgg cgcagcaggg cagcgaggtc gtcgttacgc gcgccaacga agagaagcag
      841 acgcgcgcgt cgcacggcac cacgcgggcg cacatcgcca acatgatgcg cggcgtcacg
      901 gaaggtttct cgaaggacct cgagatcgtg ggcgtgggct acaaggccga ggccaaggga
      961 aagtcgctgg tgctgacgat cggctattcg catctggtcg acttcaagat tccggacggg
     1021 atcaccgtcg agaccccgga gccgaccaag atcaaggtga gcggcatcga tcgccagcgc
     1081 gtgggccagg tcgcggccga gattcgcgcg gtgcgcgggc cggaacccta caagggtaag
     1141 ggaatcaagt acgtcgacga gcacatcgag cgcaaggccg gtaagtcggc ggcgagcggc
     1201 ggctagtcgg ttcgtcgacg ggaggcgtca tcatgcagaa gaagttcaag accatcgaag
     1261 actataagcg ctggcgtcgc cacaagcgcg ttcgtaaccg cgtcgtgggc accatggagc
     1321 gtccgcgtgt gtgcgtgttt cgcagcaacc agcacatcta cgcgcagctg atcgacgacg
     1381 agaccggtcg cacgctcctg gcggtgtcga gcctcaagat ggaggcgccg gcggcggcca
     1441 agaaggcgga agcgaaggcc gagaaggccg acgccaaggc ggagggaaag aagggcggca
     1501 aggcggccaa ggccgacaag ccggccaagg gcgagaaggc ggcggcgccg gcgggcggca
     1561 agaagaccgc actcgcgcgc gaagtcggcc gcttgctggc cgaagccgcg caggccaagg
     1621 gattcaagaa ggttgcgttc gatcgcggcg ggtacttgta ccacggtcgc gtggcggcgc
     1681 tggcaacatc agcgcgcgag cacgggctcg atttctaatc gacgcgcaag cggcatgatt
     1741 cggaggacga actagtggaa accggaacca acgagcgcac accgaccaca ccgaccacgc
     1801 cgggatcgcc ggcgacaccc gatcgtcccg cgcgccccgg cggcggcggt ggacgaggcg
     1861 gcgaccgcgg ccgagggcca cgcgggggcg gcggtggcca gggacgcggc aaggacgatc
     1921 gaggacgccg cggcggtccg cgtgagcgcg agcgcgagcg cggtgggccc gagtacatcg
     1981 agaccacggt ctacgtcaat cgcgtggcca aggtcgtcaa aggtggacgc cgtttcagct
     2041 tcaacgcgct cgtgtgcgtg ggcgacggca tgggccgcgt cggcatcggg ctcggcaagt
     2101 cgaacgaaat cagcgacgcc atccgcaagg cgggcgagaa cgcgaaacgc tcgatgatcc
     2161 gcgtgcccct gatgggcggc accattcctt accaggtgta cgggaaattc ggcgccggca
     2221 aggtgatcct gaagccggcc acaccgggta ccggcgtcat cgcgggcggt gccgtgcgcg
     2281 ccatcatgga gtccgcggga gtgcgcgacg tgctcgcgaa gtcgctgggt tcgaccaacc
     2341 cgcacaacct gatcaaggcc accatgaccg ccttgcagca gctcgagagc gccaaggacg
     2401 tcgccaagcg gcgcggtatc tcgatcaatc agttgttcgg aatcgaggcc aagaatggct
     2461 aagaagattc gcatcacgca gatcaagagc gtcatcggcg taccgccgct tcacaagaag
     2521 accatgacgg cgctcggatt tcggcgcacg taccagacgg tggaaaaggc cgacaccccg
     2581 caggtgcgcg gcatgatcca tcaggtgcgt tacctcgtta gagtcgagga gggcaagtaa
     2641 ccatgcgact cggagaactg aagcccgccg cgggctccaa gaagaagcgc aagcgcatcg
     2701 ggtgcggccc cggctccggc cacggcaaga cctcgacgcg cggtcacaag gggacccagt
     2761 cgcgcaccgg caacaccaag aacgcctggt acgaaggcgg ccagatgccg ctccagcgtc
     2821 gcttgcccaa gcgcgggttc accaacatct ttcggatcgg ctatcaggtg atcaatctcg
     2881 gcgacctgga tcgcttcgac gccaacgcga ccgtcgacgt cgacgcgctc ctcgagaagg
     2941 gtctcattcg tcatctgaac aatccggtca agctattggc ggacggcgag ctcaagaagc
     3001 cgctcaccat caaggtgcac gccgccagca agaaggccaa ggaagcagtg gagaaggcgg
     3061 gcggacaggt gaacctcgtc ggttgacggg ttctcgctga tcgttctttg acaactatat
     3121 gttcaccaag attcaggaga tcttcagcat ccccgaattg aagcgccgcg tgatcttcac
     3181 gctggggctt ctgatcgtgt atcgcatcgg cagtcacatc acgacgccgg gtgtcgatgc
     3241 ctccgtgctg accgagttct tcgcccgcca gcgcgggacg atcctcggac tgtatgacct
     3301 cttcgcgggc ggcaacctgg agaaggcgac catcttcgcg ctgggcatca tgccgtacat
     3361 cagtgcgtcg atcatcttgc agctcttcca ggccgtcatt ccctacttcg agaaactggc
     3421 gaaggaaggc gaagaaggcc gcaagaagat cacccagtac acgcgctacg ggacggtggt
     3481 gctctcgatc gtgcagggct tcggcatcgc gatcttcctg gagagcatga cgggtccgag
     3541 cggcacgccg gcggtgatgt tctcgggctg gggcttccgc atcctgaccg tcattacctt
     3601 gaccgcgggc accgtgttcg tgatgtggct cggcgagcag atcaccgagc gcgggatcgg
     3661 caacggcatc tcgctcatca tcacggtggg tatcctggcg cgctttccga tcgacgcgct
     3721 caacatgttc cgcgcgatcc agttgggaca gatgaccccg ttcaaggccg tgatcctggg
     3781 attgatgatg gtcctgatca tcgcgggcgt catcttcgtg acccaggggc agcggcgcat
     3841 ccccgtccag tacgccaagc gcgtggtggg ccggcgcgtc tacggcggcc aggccagcca
     3901 cattccgctg tcggtcaaca tcgcgggcgt gattccgatc atcttcgcgc agtccttgat
     3961 catgttcccg ggcaccatcg cggcgttgtt cccgaacgtg actattctgc agtcgatcac
     4021 gctgtggctc tcgccggggc actggatcta catcgtcctg tattcgttga tcatcatctt
     4081 cttcacgtat ttctacaccg cgatcgtctt gaatccggtc gacctcgcgg acaacatgaa
     4141 gaagtcgggc ggtttcatcc cgggaattcg cccgggcaag cgcaccgccg agtacatcga
     4201 tcgtattttg acgcgcatca cgctgccggg agcgtttttc ctggcgttta tcgcggtgct
     4261 gccggatctc ttgatcacga aggccaacgt gccgttctac ttcggcggca ccggtttgct
     4321 gatcgtggtg ggcgtgatgc tggatacatt gcgccaggtg gaatcgcacc tcttgatgcg
     4381 ccactacgaa ggcttcgtca agcacgggcg attgaagggc cgccgaggct agatgaacct
     4441 ggtgctgttc gggcccccgg gatccgggaa gggcacgcag gcggagcgac tgcggaatcg
     4501 ctggaagctc cggcacattt cgaccggaga cctcttgcgc gaagcggtcg ccgcgaagag
     4561 cgagctcggc aagaaggtgg agagcgtact cgcgtcgggg cagctggtgt cggacgacat
     4621 catcctcggt ctgatgcgcg gcgcaatcct ggcggtgaag caagacgcgg cactgtccgg
     4681 ctggttgctg gacggattcc cgcgcacggt cggacaggcg aaggggctcg atgcgctcct
     4741 cgccgagacc ggttcgcgca tcgacgcgat cgtcgtgctc gacgcgacgc gcgaagcggt
     4801 ggtcgatcga ctgtcgggtc ggcggacctg cacgaagtgc aagaccgtct accacatcac
     4861 gcacaacccg acgcgcgtcg acggcgtgtg cgacaagtgc ggcggcaaag tggtccagcg
     4921 cgaggacgat cgtccggaga cgatcgcgaa acggctggac gtgtacgaag cgcagacgcg
     4981 gcctattctc gcgcactatg cggggcgcgt cgcgacacac gtgatcgacg gcgatctgcc
     5041 ggtcgacgac gtgacggcgg agatcgagcg catcgtagga cgggcagcgg catgatcatg
     5101 ctgaagacgc tcgatgagat cgacatcatg gaggccaacg gcgccatcgt ggtcgagtgc
     5161 ttcgccctgg cgcgggattt catcaagccc gggcgcacgc gcaaggagct ggacgccgcg
     5221 atcgagaagc tgatcgtcga acggggcggg aagccggcct tcaagggctt ccacggctac
     5281 cccgcgtcga cctgcatctc ggtcaacgac gaggtcgtgc acgggattcc cgacgaccgc
     5341 gcgttccagg aaggcgattt ggtcggtctc gacatcggcg tcttcaagga cggctactac
     5401 gtcgactcgg cgcgcacgat tccggtcggg cgtgtcggca aggacgcgga gaagctgctc
     5461 gaggcgacct gggatgcgct cgcgaaggga atcgagcaag cgcgaccggg caaccggctg
     5521 accgacattt cgcacgcggt cgaagcgcgc gcgcgcaaga gcggcttcgc ggtggtgaga
     5581 tcgctggtgg gtcacggcat cggcaagcgc atgcacgagg acccgcaggt gcccaacttc
     5641 ggcccgcccc accagggacc ggtgctcgag gagggcatgg tgctcgcgat cgaaccgatg
     5701 gtgaacgtgg ggacggccga cgtgttcacg ctggaggatg gttggaccat cgtcacggcg
     5761 gatcgcaagt tgtcggcgca tttcgaagac acggtggccg tgacgaagaa cggcccccgc
     5821 atcttgacga ggtaagcgcg aaaggacgta tgccgaagga cgacagtatc acagtcgaag
     5881 ggaccgtcgt cgaggcgctg ccgaacacga tgttccgggt ggaattggcg aacaagcaca
     5941 tcgtgctcgc ccacgtgtcc ggcaagatga gaatgcattt catcaagatc ctgcccgggg
     6001 atcgagtgac cttggagatt tccgcgtacg acccgaccaa gggccggatc gtgtatcgat
     6061 acaagtagag gattgtcatg aaagttagaa cgtcgatcaa gaaaatctgc ccggattgca
     6121 agctcatccg gcgcaagggc gtgttgcgcg tgatctgcaa gcacaagcgc cacaaacagc
     6181 gccagggcta ggcccgacgc taggatcaga ggagaacaat gcgaatcgca ggcgtggaca
     6241 ttccgaacga gaagcgcatc gtcatcgcgc tcacgtatat ctatggcatc ggcccgaagt
     6301 cggcggaaac gatctgcaag aaggccggca tcagccccga gacgcgcgtg aagtccatcg
     6361 gtgaggacca gggcatcaag ttgcgcggca tcatcgagaa cgacttcaag gtcgagggcg
     6421 aattgcgcac cgagttgagc ttcaacatca agcgcctgat cgacatcaat tgttatcgcg
     6481 gccggcgtca ccggctgaat ctcccggtcc gcggccagcg cacgcgcacc aacgcgcgca
     6541 cgcgcaaggg cccgaaggtc cgcccgggcg tgggtaagaa gaaggcaccg gccaagtgag
     6601 tcac
//
LOCUS       DASPGK010000393        11190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_681382, whole genome shotgun sequence.
ACCESSION   DASPGK010000393 DASPGK010000000
VERSION     DASPGK010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681382"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VEC56_10840"
     CDS             <1..559
                     /locus_tag="VEC56_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14688.1"
                     /translation="DTLNVAAWQTEPSFGAHAFDYDRGEPDHRVGSLTPVSSEVLVRD
                     DRGLVRATVRRAGESARIVSTVLLGGSTGRLGTTREGFVASVLALFDTQPVTKSPPAA
                     LRVVASYPNPVRDAAELLVEAPAAGAASVTVYDVAGRRVSSASARLSAGSNTLRVSAP
                     RASGHYFVVVESGGATARGRFLVLR"
     gene            622..1191
                     /locus_tag="VEC56_10845"
     CDS             622..1191
                     /locus_tag="VEC56_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14689.1"
                     /translation="MRTTRLVLLASLLVAMIFATGQALAGIPNVELITALAFVSGYLL
                     GPALGAAVGAAGMGAHSLFNVMGAVAPPVWLAQVACFALVGCAGGLLGPVLARARGRV
                     RAAALAAAVGALLVLVYQLVVNVVAFYTFANDVEVWVYVWGGVAFGAMQVAWNAAFFA
                     VAMPPMLRVLARHRRELRAGAGQEGEHPR"
     gene            1188..3002
                     /locus_tag="VEC56_10850"
     CDS             1188..3002
                     /locus_tag="VEC56_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV14690.1"
                     /translation="MTRARPPAALLALAVSLAPAVARAQATDSLVAAVARGAGFENFD
                     RIAREHYLRRHAFSLDHFLEFEPGGFLVRLGPIGNEASYSRWGIGRGRALLRMNGIVL
                     NDPQDDSPPLVHTPTSGLGSLSLAGDAGVPRIEGVVDIAESPSPSGRPNTYLELSKGT
                     NDVRQRRVRFSSEASKVGIDLAYDEVLDDGYAFDASGDVPYTVDYGSARSRQSTIVLR
                     GDPDASANFELGVRGFTSTTQGDLTSNIAEGRKSGHLVWVDAGVEETRLTLYARGYKS
                     TAADSSAKNEAVGALASWRHSGPASSTLALRANVEGTDAFQDVGATADDHLVRASVDV
                     DASRAVGERGTLSAAVTVAGDEETPFAWSARARARREGSRAALGVSLARSFRLPNFGE
                     RYLPAHTNAGRTLAGSGDVDPETAWEVAGDWGLRLGGSAVNRVRASWIQAEDAIAFRP
                     RAVGTETWHVASNASDASSMWFVEERVTGDWKASAFRIRADAAVLYTSGDRTEGFASV
                     PRFQTHAAFMLGRDFFEATSALFVGARFMSADSRVDYDGNRLPSFQVWNIEVEGRLLD
                     ARLYVQYLNVTDEQYRTQSDYLMTPGSLVYGIEWTMFD"
     gene            3033..3410
                     /locus_tag="VEC56_10855"
     CDS             3033..3410
                     /locus_tag="VEC56_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXV14691.1"
                     /translation="MRRTMGATMLIMVLGGATVVAWWSGRTAEKTPVDTVRTTPDSTL
                     TPAARPAPPAGIDFLADPLAFLSTAPADSLELLPGVGPVLATRIVAARRDRGPFRSWD
                     DVDRVRGIGPRTIERLKALSTRL"
     gene            3726..5411
                     /gene="pilB"
                     /locus_tag="VEC56_10860"
     CDS             3726..5411
                     /gene="pilB"
                     /locus_tag="VEC56_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HXV14692.1"
                     /translation="MRDRIAQKLVESSLVSQDQLARALESQQTGGGTLSYNLVKTGAI
                     SEMAFAEFMGQVYNVPAVDLDAVLVDPNAVDLIPADVATKFQVVPLKREGRTLTLAMA
                     NPDNIFAIDDIKFITGFDVRPVVATETAIKRCIDRMYDSADSLATIMGEIEEDFEIVE
                     DAIDDAELSAAAAAADAPVVKLVNSLISDAVGRGASDIHIEPYEKQLRVRFRIDGMLH
                     EMMSPPFKMKNAITSRLKIMAELDIAEKRVPQDGRIKIRMHGKPIDLRVSTLPTIFGE
                     KVVMRILDQSNLQIDLARLGFHPGALGRFLNAIESPYGMVLVTGPTGSGKSTTLYSAL
                     QKINKIHSNIMTCEDPVEYNINGINQVNVQEEIGLTFAAALRAFLRQDPNIIMVGEIR
                     DLDTASIATKAALTGHLVLSTLHTNDAASSINRLVDMGIEPFLVSSSVLLIIAQRLVR
                     RLCEKCKQPTNIHEEVLRELGIMENPDELTLMEAKGCPVCNDTGYKGRVGVYEVMEIS
                     PTLREMIVDRASNAEIKAQAIKEGMLTLRMDGLEKFKAGLTSLEEILRETANH"
     gene            5465..6541
                     /locus_tag="VEC56_10865"
     CDS             5465..6541
                     /locus_tag="VEC56_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXV14693.1"
                     /translation="MINLRDLLQEMIEKGASDLHLTAGLPPMIRVDGEVLPTDREALT
                     PEQTQALAYSVLNEEQQKRFESVKELDFSFGVKGMSRFRGNAYLQRGCMGLAIRQIPY
                     EIRTFRDLGLPAVMEQLAKKQQGLILVTGATGSGKSTTLASIIDKINSERRRHIVTIE
                     DPVEYIHQHKKCIVNQREVKADTETFSNALKHVLRQDPDVILIGEMRDQETMAAALTI
                     AETGHLTLATLHTNSTFESINRIVDTFPPGQQNQVRAQLAFVLEGVLAQQLVPRARGK
                     GRVLVTEVMVCTPAIRAVIRDDKVHQIYGLMQAGQKHGMQTMNQALFNAVVSKEISLE
                     EAMRRSLDQNELSTMLGQPVGAGR"
     gene            6580..7797
                     /locus_tag="VEC56_10870"
     CDS             6580..7797
                     /locus_tag="VEC56_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV14694.1"
                     /translation="MATTFVWKGRSPNGELLSGEYQAETKEDLVSHLRKRKIIITSMR
                     EKAKGNLNIKLPGQDRVSVKDIGVFTRQFATMINAGLPMVQCLDILAQQTEKEFFRTS
                     IAKIMSDVEGGSTLAESMGRHPKVFSTLYVNMVEAGEAGGILDVILVRLATFLEKLDQ
                     LQRKVKSALTYPSVVAFVAIMATCFMLIFIIPTFAKMFTDFGGQLPTPTRIVMGLSDF
                     LRGYWWLIIAIITGVVIAIQRYYKTESGRLVIDKLLLRIPVLGTVIRKGAVARFTRTL
                     GTLISSGVPILSGLEITARTAGNRVVENAVIATRESISQGNTIAEPLRASGVFPPMVT
                     QMIAVGEQTGALDEMLEKIATFYDSEVDTAVDALTAIIEPVMIVVMGTVVGGMLVAMY
                     LPMFKLVAVVSGG"
     gene            7814..8176
                     /locus_tag="VEC56_10875"
     CDS             7814..8176
                     /locus_tag="VEC56_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14695.1"
                     /translation="MDAVRTNAASGGAKSHSTPALRAHASVLTGDPQEFERFLVLRSI
                     VTALIVGAGVTIVELTDELTRVGPLYVLLGASVVVGVIAAGLTRLKVPVARSAWVVVV
                     ADVALVAGIMHYSGGVGG"
     gene            8186..9592
                     /locus_tag="VEC56_10880"
     CDS             8186..9592
                     /locus_tag="VEC56_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV14696.1"
                     /translation="MFCLAIAAAAFLLAMPGGLAAALLSSACFVVYQILEAQGIVDPP
                     GRELYAKGRASGIIDAYMHTSMFLLVGTVGGYLADRIRLKGRALQHAETALEQLRVDT
                     NYILENMSSGVLVIDSVCRVVTMNLAAEEILELKKDSVLGRHIDEALGRRVPDLAHEL
                     VQSLSMERGKHRHEIAAHSRDGRHRPLGISISLLTDAAERRRGVIAVFQDLTEVHEMR
                     ERVRKADRLAAIGELSAGIAHELRNPLASISGSIEMLHQELKLDGENGRLMELIMRES
                     DRLDRIISDFLDFAKIRQPKKLPARLDKCLSETMVLLKKNADKSQGISIRLECAEDMP
                     LVYMDDEQMRQVFMNLAVNSCEAMEKGGTLDVVAGPTGAGQVRVAFCDSGPGISPEGM
                     ERLFEPFFTSKEGGTGLGLAIANKIVAAHGGVIEFRNREQGGAVFTITLPIVGSGEHP
                     ARPEKKKNSKQAVAAVAD"
     gene            9622..11013
                     /locus_tag="VEC56_10885"
     CDS             9622..11013
                     /locus_tag="VEC56_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV14697.1"
                     /translation="MAGERILVVDDEKSMCQFLSIMLRKEGYDITTVSTGKKAVEEVS
                     ASKFDVVLTDIKMAGMDGMEVLKEIRRLDATMPVIIMTAYASQKTAIEAVNQGAFHYL
                     IKHAKNDEIKMVVRNALDMKRVRTENVLLKKQLKKSSDLRTIIGKSEEMQKVFQLVDK
                     VADTESTILIYGESGTGKELIARAIHYRSSRSAGPFVSINCGALPESLLESELFGHVK
                     GSFTGAIRDKEGLFKVAQGGTFFLDEIGETSPTIQVKLLRVLQEREVIPVGGTNPIKV
                     DVRLVAATNADLEKAIKQERFRADLYYRLNVIPVQLPPLRKRRDDVPLLVQHFLRKYN
                     EDLPTERQKGITRDAMEVLVNYEWPGNVRELENVMERAVILEETDQITVGSLPEKVRQ
                     RESGASHLITERAQVTLDELEKEYLIKVLNDTNWQKKKASQILGINASTLYRKIQRYG
                     LDKELEKELIESE"
BASE COUNT         2006 a         3581 c         3772 g         1831 t
ORIGIN      
        1 ggacacgctg aacgtcgccg cctggcagac ggaaccgtcg ttcggcgcgc acgcgttcga
       61 ctacgaccgg ggcgagcccg atcaccgcgt gggcagcttg acccccgtgt cgtccgaggt
      121 gctcgttcgc gacgatcgcg ggctggtgcg cgcgaccgtg cggcgcgcgg gtgagtcggc
      181 gcggatcgtg agcacggtgc tcttgggcgg ttccacgggg cgcttgggca ccacgcgcga
      241 gggtttcgtg gcgagcgtgt tggcgctctt cgatacgcaa ccggttacca agtcgccgcc
      301 cgccgcgctg cgcgtggtgg cgtcgtaccc caacccggtg cgcgacgcgg ccgagctcct
      361 ggtcgaagca ccggcggccg gggcggcctc ggtgacggtg tacgacgtcg ccggccggcg
      421 ggtatcgtcg gcgtccgcgc ggctctcggc cgggtcgaac accctgcgcg tgagcgcccc
      481 gcgcgcgtcc ggacactact tcgtggtggt cgaatcggga ggggcgaccg cgcggggtcg
      541 tttcttggtt cttcgttgac gcttagcgca ctcgcttata gcatcgcgcg aaccgcggcc
      601 cctgggcgcg gtggacctgc gatgagaacc acccgcctcg tgctgcttgc gagcctcctg
      661 gttgcgatga tcttcgcgac cgggcaggcg ctcgccggca ttcccaacgt cgagttgatc
      721 accgcactcg ccttcgtgtc ggggtatctg ctcgggcccg cgctgggggc ggcggtgggc
      781 gcggcgggga tgggcgcgca ctctctattc aatgtgatgg gcgcggtggc cccgccggtg
      841 tggcttgcgc aagtggcgtg cttcgcgctc gtcgggtgcg cgggcggact gcttgggccg
      901 gtgctcgcgc gcgcgcgtgg gcgcgtgcgt gcggcggccc tggccgccgc ggtcggcgcg
      961 ttactcgtcc tggtttacca actggtcgtc aacgtggtcg cgttctacac cttcgctaac
     1021 gacgtcgagg tgtgggtgta cgtgtggggc ggggtcgctt tcggtgcgat gcaggtggcg
     1081 tggaacgcgg ccttcttcgc cgtcgccatg ccgcccatgc tgcgcgtgct cgcgcgccat
     1141 cgccgcgaat tgcgcgcggg tgccgggcag gaaggggagc acccgcgatg acgcgcgccc
     1201 gcccacccgc cgcgctcctc gcgctcgcgg tgtccctggc accggcggtc gcgcgcgcgc
     1261 aggccacgga ctccctggtc gccgcggtcg cgcgcggggc ggggttcgaa aacttcgacc
     1321 gcatcgctcg cgagcactat cttcgccgcc acgcgttctc gctcgatcac ttcctcgagt
     1381 tcgagccggg cgggttcctg gtgcgcctgg ggccgatcgg gaacgaggcg tcgtactcgc
     1441 gctggggcat cggacgcggg cgtgcgctcc tgcgcatgaa cggcatcgtg ctcaacgacc
     1501 cgcaggacga ctcgccgccc ttggtgcaca cgcccaccag cgggctgggt tcactctcgc
     1561 tcgcgggcga tgcgggcgtt ccccggatcg aaggggtggt ggacatcgcg gagtcgccgt
     1621 cgcccagcgg gcgaccgaac acctacctcg agctttcgaa agggaccaac gacgtgcgcc
     1681 agcggcgcgt gcgcttttcc tcggaggcat cgaaggtcgg catcgacctc gcctacgacg
     1741 aagtgctcga cgacggctat gcattcgacg cgtccggcga cgtgccctac accgtcgact
     1801 acggcagcgc gcgctcgcgc cagtcgacca tcgtgctgcg cggagacccc gacgcgtccg
     1861 cgaacttcga gctgggcgtg cgcggcttca ccagtaccac gcagggcgac ctcacgagca
     1921 acatcgccga gggacgcaag agcgggcacc tggtatgggt cgacgcgggc gtcgaagaga
     1981 cgcggctcac gctgtacgcg cgcggataca agtcgacggc ggccgactcg tcggccaaga
     2041 acgaggcggt cggggcactg gcgtcgtgga ggcactccgg ccccgcgtcg agcacgctcg
     2101 cgttgcgcgc gaacgtcgaa ggcacggacg cgttccaaga cgtgggtgcg accgccgacg
     2161 accacctcgt gcgtgcgtcg gtcgacgtgg atgcgtcgcg cgcggtgggc gagcgcggca
     2221 cactctccgc cgcggtgacg gtggcgggtg acgaagagac gccgtttgcg tggagcgcgc
     2281 gcgcgcgcgc gcggcgcgag ggatcgcgcg cggcgctggg ggtgtcgctc gcgcgctcct
     2341 tccggcttcc caacttcggc gagcgctatc tgcccgcgca caccaacgcc gggcgcacgc
     2401 tggccggcag cggcgacgtc gaccccgaaa ccgcctggga agtcgccggc gactgggggc
     2461 ttcggctcgg gggttcggcc gtcaaccggg tgcgcgcgtc gtggatccag gcggaagacg
     2521 ccatcgcgtt ccggccgcgc gcggtcggga cggaaacgtg gcacgtcgcg agcaacgcgt
     2581 ccgacgcgag ctccatgtgg ttcgtcgaag agcgagtgac gggggactgg aaggcctccg
     2641 ccttccggat tcgcgccgac gccgcggtgt tgtacacctc gggcgatcgc acggaagggt
     2701 tcgcgagcgt cccgcgcttc cagacccacg cggcattcat gctgggccgc gacttcttcg
     2761 aggcgaccag cgcgctcttc gtcggtgcgc gcttcatgtc ggccgattca cgcgtcgact
     2821 acgacgggaa tcgtttgccg agtttccagg tgtggaacat cgaggtcgag gggcgcctct
     2881 tggacgcgcg cctgtacgtg cagtacctga acgtgaccga cgagcagtac cgcacccaaa
     2941 gcgactactt gatgacaccg gggtcgctcg tttacggtat cgagtggacg atgttcgact
     3001 gatcgtttga cgtcgcccgg gaggaggggt ggatgcggcg gacgatgggg gcgacgatgc
     3061 tcattatggt gctgggcggt gcgaccgtgg tggcgtggtg gtcggggcgc acggccgaaa
     3121 agacgccggt cgacaccgtc cgaacgaccc ccgattccac cctcactccc gccgcgcgac
     3181 ccgcgccccc ggccggcatc gacttcctag ccgatccgct cgcgttcctc tcgaccgcac
     3241 ccgccgactc gctcgagctc ctgcccggcg ttggtccagt ccttgccacc cgcatcgtcg
     3301 ccgcgcgccg cgaccgcggt ccgttccgct cgtgggacga cgtcgatcgc gtacgcggca
     3361 tcggaccgag gaccatcgag cgtctgaaag cgctttcgac gcggctctaa gcggcacgtt
     3421 ctgccgcgtg ccgagcgcca cacgcgcgct ggcaaattgc gaatgggcgg gacgaacgca
     3481 cggcggcatt gaagtccgtg cggcgggtgc gttctcgtcg ttccccatcg tggtttgtgt
     3541 agcgcgaccg gtggcgtccc atgcgcgctc acgcgcgcga accggtggca cggtaattgc
     3601 ggaatcgcgc gcaagttgaa caggttctaa ccacttgtac gtcgtgctcg gtcgacggcc
     3661 gcgacacgct ttcggcgtcg cggccgccgt gtcgcttcgg gattcgttga cgggagggcc
     3721 agtctttgag agatcgcatc gcccagaagc tcgtcgagtc ttcgctcgtt agccaagacc
     3781 agctcgcacg cgccctcgag tcccagcaga cgggcggcgg cacgctttcc tacaacctgg
     3841 tcaagaccgg cgccatctcg gagatggcct tcgccgagtt catgggccag gtctacaacg
     3901 tccccgcggt cgacctcgac gcggtcctgg tcgaccccaa cgccgtcgac ctgatccccg
     3961 ccgacgtcgc caccaagttc caggtggttc ccttgaagcg cgaaggtcgc acgctgaccc
     4021 tcgccatggc gaaccccgac aacatcttcg cgatcgacga catcaagttc atcaccgggt
     4081 tcgacgtgcg cccggtggtg gccaccgaga ccgccatcaa gcgctgcatc gaccgcatgt
     4141 acgactcggc cgactcgctc gccaccatca tgggcgagat cgaggaggac ttcgagatcg
     4201 tcgaggacgc catcgacgac gccgagctct ccgcggccgc tgcggccgcc gacgcccccg
     4261 tggtcaagct cgtcaacagc ctcatctccg acgcggtggg acggggggcc agcgatattc
     4321 acatcgagcc ctacgagaag cagctgcgcg tgcgcttccg catcgacggc atgctgcacg
     4381 agatgatgtc gccgcccttc aagatgaaga acgcgatcac gtcgcgcttg aagatcatgg
     4441 ccgagctgga catcgccgag aagcgcgtgc cccaggacgg ccgcatcaag atccggatgc
     4501 acgggaagcc gatcgatctg cgcgtgagca cgctcccgac gatcttcggc gagaaggtcg
     4561 tgatgcgaat tctcgaccag agcaacctgc agatcgacct cgcgcgtctg ggcttccacc
     4621 cgggtgcgct cgggcgcttc ctgaacgcga tcgagagccc gtacgggatg gtgctggtga
     4681 caggccccac gggttccggc aaatcgacca cgctgtacag cgcgctccag aagatcaaca
     4741 agatccactc gaacatcatg acctgcgagg acccggtcga gtacaacatc aacgggatca
     4801 accaggtcaa cgtgcaagaa gagataggac tcacgttcgc cgccgcactg cgcgccttct
     4861 tgcgtcagga ccccaacatc atcatggtcg gtgagattcg agacttggac acggcgtcca
     4921 tcgccaccaa ggctgctctc accggtcact tggtcttgag caccctgcac accaacgacg
     4981 cggccagctc catcaatcgt ctggtcgaca tgggcatcga gcccttcctg gtgtccagct
     5041 cggtgctgct catcatcgcc cagcgactgg tacgacgcct gtgcgagaag tgcaagcagc
     5101 ccaccaacat ccatgaggaa gtgctgcgcg agctgggcat catggagaac ccggacgagc
     5161 tcacgctcat ggaagccaag ggctgcccgg tctgcaacga caccggctac aagggccgcg
     5221 tgggcgtcta cgaagtcatg gagatctcgc ccaccttgcg cgagatgatc gtcgatcgtg
     5281 cctccaacgc agagatcaag gcgcaggcga tcaaggaagg catgctgaca ctgcgcatgg
     5341 acggcctcga aaagttcaag gcgggcctga cgtcgttgga agagattctg cgcgagacgg
     5401 cgaaccacta ggcgcgacac ccttgcgcgg ctcgatacaa cgaataccga ggagatagaa
     5461 gaacatgatc aaccttcgcg atttgctgca ggaaatgatc gaaaagggcg cgtcggacct
     5521 gcacctgacg gccgggttgc cgcccatgat ccgcgtcgac ggcgaagtgc tgccgaccga
     5581 tcgcgaggcg ctcacccccg agcagacgca ggcgctcgcg tacagcgtac tgaacgaaga
     5641 gcagcagaag cgattcgagt ccgtcaagga gctcgacttc tccttcggcg tcaagggcat
     5701 gagccgcttt cgcggcaacg cgtacctgca gcgcggctgc atgggactcg cgattcgtca
     5761 gattccgtac gagatccgca cctttcgcga cctggggctt cccgcggtca tggaacagct
     5821 ggccaagaag cagcagggcc tgatcctcgt caccggtgcc accggcagcg gtaagtcgac
     5881 cacgctcgcg agcatcatcg acaagatcaa cagcgagcgc cgccgccaca tcgtcaccat
     5941 cgaggatccg gtcgagtaca tccaccagca caagaagtgc atcgtcaacc agcgcgaggt
     6001 gaaggccgac accgagacct tctccaacgc cctcaagcac gtgctgcggc aggaccccga
     6061 cgtcattctg atcggtgaga tgcgcgacca ggagaccatg gcggcggcgt tgaccatcgc
     6121 cgagacgggc cacttgacgc tcgccacgct gcacaccaac tcgaccttcg agtcgatcaa
     6181 ccgcatcgtg gatacgttcc cgcccggcca gcagaaccag gtgcgcgcgc agctggcgtt
     6241 cgtactggag ggcgtgctgg cccagcagct cgtgccgcgc gcgcgcggca agggccgcgt
     6301 gctcgtcacc gaagtgatgg tgtgcacgcc cgccatccgc gcggtcatcc gcgacgacaa
     6361 ggtgcatcag atatatggac tcatgcaggc cggccagaag cacggcatgc agaccatgaa
     6421 ccaggcgttg ttcaacgcgg tcgtgtcgaa ggaaatctcg ctggaagaag cgatgcgccg
     6481 cagcctcgac cagaacgagc tgtccaccat gctgggccag cccgtgggtg cggggcgcta
     6541 agcgcgtccg acgacgcgat ctcgaagggg gtctcttaga tggcgacgac attcgtatgg
     6601 aagggccgca gcccgaacgg agagttgctc tccggcgagt accaggcgga gaccaaggag
     6661 gacttggtca gccacctgcg taagcgcaag atcatcatta cctccatgcg cgagaaggcc
     6721 aagggcaacc tgaacatcaa gctgcccggg caggatcgcg tcagtgtgaa ggacatcggc
     6781 gtgttcacgc gccaattcgc gaccatgatc aacgccggtc tgccgatggt gcagtgcctc
     6841 gacattctgg cccagcagac cgagaaggag ttcttccgca cctcgattgc gaagatcatg
     6901 tcggacgtgg aaggcggctc cacgctggcc gagtcgatgg ggcgccaccc caaggtgttc
     6961 agcacgctct acgtcaacat ggtggaggcg ggcgaggcgg gcggtattct ggacgtcatc
     7021 ttggtgcgac tggcgacgtt cttggaaaag ctcgaccagt tgcagcgcaa ggtcaagagc
     7081 gcgctcacct atccaagcgt ggtcgccttc gtggccatca tggcgacgtg cttcatgctc
     7141 atcttcatca ttccgacctt cgcgaagatg ttcaccgatt tcggcggaca gctgccgacg
     7201 ccgacgcgca tcgtcatggg cttgagcgac ttcctgcgcg ggtactggtg gttgatcatc
     7261 gcgatcatca ccggcgtcgt gatcgcgatc cagcgctact acaagaccga gagcggccgg
     7321 ctggtcatcg acaagttgtt gctgcgcatc cccgtgctcg gcaccgtgat ccgcaagggc
     7381 gcggtggcgc gcttcacgcg cacactggga accctcatct ccagcggcgt gcccattctg
     7441 tcgggcctcg agatcacggc ccgcaccgcg ggcaaccgcg tggtggagaa cgccgtgatc
     7501 gcgacccgcg agagcatcag ccagggtaac accatcgccg agccgctgcg cgcgagcggg
     7561 gtatttccgc ccatggtgac gcagatgatc gcggtcggcg agcagacggg tgctctcgac
     7621 gagatgttgg aaaagatcgc gaccttttac gacagcgaag tggacacggc cgtcgacgcg
     7681 ctcacggcca tcatcgaacc ggttatgatc gtggtcatgg gtaccgtggt cggcggcatg
     7741 ctggtcgcga tgtacctgcc gatgttcaag ctcgtcgccg tcgtgtccgg cggttagtcg
     7801 aagtacggtc gacgtggacg cggtccggac caatgccgcc tccggcgggg cgaagtcgca
     7861 ttcgaccccc gctttgcgcg cgcacgcgag cgtcttgacc ggcgacccgc aggagttcga
     7921 gcgcttcctg gtgcttcgca gcatcgtgac ggcgctgatc gtgggcgccg gggtcacgat
     7981 cgtcgagttg accgacgagc tcacgcgcgt gggaccgctc tacgtgctcc tgggcgcgag
     8041 tgtggtggtg ggcgtgatcg cggccgggct cacccggctc aaggtaccgg ttgcgaggtc
     8101 ggcgtgggtg gtggtggtgg cggacgtggc cctggtggcc ggcatcatgc actacagcgg
     8161 cggcgtgggg ggatagttca cgaccgtgtt ctgtctcgca atcgccgccg cggcgtttct
     8221 gctcgcgatg ccgggtgggc tggccgcggc actcctctcg tcggcgtgct tcgtggtcta
     8281 tcagattctg gaagctcagg gcatcgtcga cccgccgggt cgcgaactgt acgcgaaggg
     8341 gcgcgcgagc gggatcatcg acgcctacat gcacacctcg atgttcctcc tggtgggcac
     8401 ggtgggcggg tatctggcgg atcgcattcg cttgaagggg cgcgcgctgc agcacgcgga
     8461 gaccgcactc gagcagctgc gcgtcgacac gaactacatc ctggagaaca tgagcagcgg
     8521 cgtgctcgtg atcgactcgg tgtgccgtgt ggtgacgatg aacctggcgg cggaagagat
     8581 tctcgagctc aagaaagact ccgtactggg gcgacacatc gacgaggcgc tggggcgtcg
     8641 ggtacccgac ctcgcgcacg aactggtgca gtcgctctcg atggagcggg gcaagcaccg
     8701 ccacgagatc gcggcccact cgcgggacgg ccgccatcgc ccgctgggca tcagcatctc
     8761 gctcttgacc gacgccgcgg agcggcgccg gggggtgatc gcggtcttcc aggacctgac
     8821 cgaggtccac gagatgcgcg agcgcgtgcg caaggcggac cgtctggcgg ccatcggcga
     8881 attgtccgcg ggcatcgcgc acgagctgcg caacccgctg gcgtcgatat cggggtcgat
     8941 cgagatgctc caccaggagc tcaagctcga cggagagaac gggcgcttga tggagctcat
     9001 catgcgcgag tcggaccggc tggatcgcat catctccgac tttctcgatt tcgcgaagat
     9061 acgccagccc aagaagctgc ccgcgcggct cgacaagtgt ctttcggaga ccatggtgct
     9121 tctgaagaaa aacgcggaca agagccaggg gatctcgatc cgactcgagt gcgcggaaga
     9181 catgccgctc gtttatatgg acgatgagca gatgcgccag gtgttcatga acctcgccgt
     9241 caattcgtgc gaggcgatgg agaagggcgg gacgctggac gtggtggcgg ggccgaccgg
     9301 ggccgggcag gtgcgcgtcg ccttctgcga cagcggtccc ggtatctcgc cggagggcat
     9361 ggagcgtctc ttcgagccgt tctttaccag taaggaaggc gggaccggtc tgggcctcgc
     9421 tatcgcgaac aagatcgtgg cggcgcacgg cggcgtgatc gagttccgga atcgcgagca
     9481 gggcggcgcg gtgttcacca tcacgctccc gatcgtcggc agcggcgagc acccggcgcg
     9541 ccccgagaag aagaagaata gcaagcaggc cgtcgcagca gtggcggact agagagcaat
     9601 tcacgagaag ggaagacacg catggctgga gaacgcattc tggtggtcga tgacgagaag
     9661 agcatgtgcc aattcctttc catcatgctg cgaaaggaag gctacgacat caccaccgtc
     9721 agcaccggca agaaggcggt ggaagaagtc tcggcgagca agttcgacgt cgtcctgacc
     9781 gacatcaaga tggcgggcat ggacggcatg gaggtcttga aggagatccg ccgcctggac
     9841 gccaccatgc cggtcatcat catgaccgca tacgcgtccc agaaaaccgc gatcgaggcg
     9901 gtcaaccagg gcgccttcca ctacctcatc aagcacgcca agaacgacga gatcaaaatg
     9961 gtcgtgcgca atgcgctcga catgaagcgc gtgcgcaccg agaacgtgct cttgaagaaa
    10021 cagctcaaga agagctcgga cctgcgcacc atcatcggca agagcgaaga gatgcagaag
    10081 gtgtttcaat tggtcgacaa ggtggccgac accgagtcca cgatcctcat ctacggagaa
    10141 agcggcaccg gcaaggagct catcgcgcgc gcgatccact atcgctcgtc gcgctcggct
    10201 gggcccttcg tcagcatcaa ctgcggggcg ctgccggaga gcctcctcga gagcgagctg
    10261 ttcggacacg tgaaggggtc gttcacgggt gccatccgcg acaaggaagg tctcttcaaa
    10321 gtggcccagg gcggcacctt cttcctggac gagatcgggg agacctcgcc caccattcag
    10381 gtcaagctcc tacgcgtcct gcaggagcgc gaagtgatcc cggtcggcgg caccaacccc
    10441 atcaaggtcg atgttcggct tgtggcggcc accaacgccg acttggagaa agcaatcaag
    10501 caggagcgct tccgcgccga cctctattac cgcctgaacg tgatcccggt ccaactgccg
    10561 cccttgcgca agcgccggga cgacgtgccg ctcctcgtgc agcacttctt gcgcaagtac
    10621 aacgaggacc tgcccaccga gcgccagaag ggcatcacac gggacgccat ggaagtcctg
    10681 gtcaattacg agtggccggg taacgtccgc gagctggaga acgtgatgga gcgggcggtg
    10741 attctcgagg agaccgacca gatcacggtg gggtcgctcc cggaaaaggt ccgccagcgc
    10801 gagtcggggg cgagccattt gatcaccgag cgcgcgcagg tgaccctcga cgagctcgag
    10861 aaagagtacc tcatcaaggt actcaacgac accaactggc agaagaagaa agccagccag
    10921 attctgggga tcaacgcgtc gacgttgtat cgcaaaatcc agcgctatgg cttggacaag
    10981 gaactggaga aggaactgat cgaaagcgag tagcgtgcat cccggcaaac tgcactttgc
    11041 ggcaccggga cgccgcgcgc cgcaacttcg atatccgcgc cgcggggcag gaacggcgag
    11101 cgacgcggca gggcctagag gaacaaacgg gaacggaaag agcgggcaaa tcgagttggc
    11161 ataccaattg ccttaacgtg ggccaggacc
//
LOCUS       DASPGK010000394         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1266823, whole genome shotgun sequence.
ACCESSION   DASPGK010000394 DASPGK010000000
VERSION     DASPGK010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266823"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VEC56_10890"
     CDS             <1..484
                     /locus_tag="VEC56_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14698.1"
                     /translation="PVPAAGLNSATEHDQLPFVARTGGGFTMVWVRHDTSQPKPWLNP
                     KSQLYYATSGDGLAWSTPLQVTNDAGNIVHLFPSIVTRSSGPPSILWLSNRGGDARPY
                     ELSVSNVDQYPVGMDDVSLFPDGYSHRMAETPIGGLFLGAWVQGPDGEQEVFYRFFER
                     "
     gene            490..1005
                     /locus_tag="VEC56_10895"
     CDS             490..1005
                     /locus_tag="VEC56_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HXV14699.1"
                     /translation="MHQHVQFARVVALACVLLVAACGPRKTPEFEPSPGVYATPDGIK
                     FTVKVTPARFALGEPVSLEASMFNGGRDAFEKSFATGCQWDFEVAGDNGRVVAPSRFC
                     TMAGSELRLEPGELRMILREWKGNDDYFGASEPLPPGRYSVTAGFVDQHTRVVPMAEP
                     FWVEIVAPRRR"
     gene            1097..1726
                     /locus_tag="VEC56_10900"
     CDS             1097..1726
                     /locus_tag="VEC56_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14700.1"
                     /translation="MKHRIVGSLFVFLSFGAAAAHARSADSTVADVDSVAATPSSQPV
                     AAPVSAPPPAQTTATKRPLRERVYYGGSVLVSFGDVTRIGVYPMIAYKFTPKLSLGAE
                     VGYEWLKFDDFDQSASNYGGSVFARYRIVPRLYAHAEYQMVNYEIFTSPTTSDRDWVP
                     FLLVGGGFSQPMGRNAWTYVEVLFDVLNDNGSPYDDGEPFISVGVGVGF"
     gene            1752..2144
                     /locus_tag="VEC56_10905"
     CDS             1752..2144
                     /locus_tag="VEC56_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HXV14701.1"
                     /translation="MAALTAASCAIAGLAFAQAPVETAATGHAAPETDYWSMLFVLVL
                     STFIGLGVIIRVSRLLHTPLMSLTNAISAIAVVGSILVTGGEHPKEIRILGAVALFAS
                     MTNIVSGFLITDRMLKMFKQSPAGEKKS"
     gene            2144..3556
                     /locus_tag="VEC56_10910"
     CDS             2144..3556
                     /locus_tag="VEC56_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXV14702.1"
                     /translation="MHNIVQAAYLAAMVLFIFSLHGMNHPRTARRGVFCGIAAMLLAV
                     VATWMQPEVLNHGWIVVAIIAGFVVGVPLSRVPLTAVPQRTALSHAFGGLAAGLVGTA
                     KYYLWVGESPEQLTTFRMTAIVAEIILGFLTFTGSLMAAGKLQELKWIPQRPVTYRGQ
                     NIVNIGLLAIAVIAGLVLIANPTGQYAPFLFPLMMGLSLLFGVLLVIPIGGADMPTVI
                     SILNSYAGLSAVAMGFVLDNKLLITAGALDGSSGLILSIIMCRAMNRSFTNVLFGAFG
                     QVQAAKAGAEQKVYKPETIEGAAQVLEQASLVVIIPGYGMAVAQAQHRVRELHDQLTK
                     RGITVKFAIHPVAGRMPGHMNVLLAEADVPYGDLVEMDEINGDMPQCDVALVVGANDV
                     VNPAARTDKSSPIYGMPIIDADKAKTVYAIKRSKNPGFAGIDNELYFADQTFMLFGDA
                     KAVVGELVKQLAREGAAAAH"
     gene            3572..4234
                     /locus_tag="VEC56_10915"
     CDS             3572..4234
                     /locus_tag="VEC56_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXV14703.1"
                     /translation="MEFLARVFDFFLHIDKHLLEVVTTYGTWTYAILFAIVFLETGFV
                     VTPFLPGDSLLFAAGALAGAGALDVVWVMVLLTIAAILGDSVNYAIGRAFGVRAFTEG
                     SRFFKEEYLLRTQRFYEKHGGKTIVLARFVPIVRTFAPFVAGVGRMRYVRFWAYNAAG
                     GVLWVVSLTLLGYFFGTIPFVKENFTVAILVIVLLSVVPVFVEMAKHRIQRSRAAAGE
                     QS"
     gene            4464..>4701
                     /locus_tag="VEC56_10920"
     CDS             4464..>4701
                     /locus_tag="VEC56_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV14704.1"
                     /translation="MSTNQRPQKGEFNEYYAGYIAKVPDGDIVSILRSQIAESTTMLE
                     RANPERVEYRYAPGKWTLHEVVGHVVDMEWVFTVR"
BASE COUNT          760 a         1543 c         1528 g          870 t
ORIGIN      
        1 tccggtcccg gccgccggtc tcaacagcgc caccgagcac gaccagctcc cgttcgttgc
       61 gcgcaccggg ggcggcttca cgatggtgtg ggtgcgtcac gacacgtcgc agcccaagcc
      121 ctggctcaat ccgaagtcgc agctctatta cgcgacctcg ggcgacgggc tcgcgtggtc
      181 gaccccgctg caagtcacca acgacgccgg caacatcgtc catctgttcc cgtcgatcgt
      241 gacccggagc tcgggcccgc cgtcgatcct gtggctgtcg aaccggggag gcgacgcgcg
      301 gccctacgaa ctctcggtct ccaacgtcga ccagtatccg gtcggcatgg acgacgtcag
      361 tctgttccca gacggttatt cgcaccgcat ggccgagacc ccgatcggcg gcctgttcct
      421 gggcgcctgg gtgcagggac cggacgggga gcaggaagtc ttttaccgat tcttcgaaag
      481 gtgaggcgca tgcaccagca cgttcagttc gcgcgcgtgg tcgcgctcgc gtgtgttctc
      541 ctcgtcgccg cctgcgggcc gcgcaagacg ccggagttcg aaccgtcgcc gggcgtgtac
      601 gcgacccccg atggaatcaa gttcacggtc aaggtgacgc cggcgcgctt cgccctgggt
      661 gagccggtgt cgctggaggc gagcatgttc aacggagggc gcgacgcgtt cgagaagagt
      721 tttgcgactg ggtgccagtg ggacttcgaa gtggcgggcg acaacgggcg cgtggtggcg
      781 ccgtcgcgct tttgcacgat ggccgggagc gaactgcgcc tcgagccggg ggaactgcgc
      841 atgatcctgc gcgaatggaa gggcaacgac gattacttcg gcgcgagcga gcccttgccg
      901 ccggggcgct actcggtgac cgcgggcttc gtcgaccagc acacgcgcgt ggttcccatg
      961 gccgagccct tctgggtcga aatcgtcgcg cctcgccggc gctaaagcat ttgcatcgga
     1021 gcgcaagctc cgttactctg tcgctcaatt atcttcgacc gggccgcggt cgggaccgag
     1081 tgccaaggag gaagtcgtga agcaccggat tgtcgggagt ttattcgtct ttttgagttt
     1141 cggcgccgca gcagcgcacg cacggtccgc agactctacg gtggcggacg tcgatagcgt
     1201 cgccgcgaca ccgtcctcgc agcccgtggc cgctccggtt tcggctcccc cgcccgccca
     1261 gacgaccgcg accaagcggc cgcttcgcga gcgtgtgtac tacggcggca gcgtgctggt
     1321 ctccttcggc gacgtgacgc gcatcggggt ctatcccatg atcgcgtaca agttcacacc
     1381 caagctctcc ttgggcgccg aggtcgggta cgagtggctg aaattcgacg acttcgacca
     1441 gtcggcgagc aactacggcg ggagcgtctt cgcgcgctat cgcatcgtcc cgcgcctcta
     1501 cgcccacgcc gagtaccaaa tggtcaacta cgagatcttc accagcccga ccacctcgga
     1561 tcgcgactgg gttccgttcc tgctggtcgg tggcggcttc tctcagccca tggggcgcaa
     1621 cgcgtggacc tacgtcgagg tcctgttcga cgtattgaac gacaacggct cgccctacga
     1681 cgacggcgag ccattcatca gcgtgggggt gggcgttgga ttctagattc acatatcgaa
     1741 cgattcttac ggtggccgcg ctgacggccg cgtcgtgcgc gatcgcgggg ctcgccttcg
     1801 cgcaggcgcc cgtcgagacc gcggcgaccg ggcacgcggc gcccgaaacc gactactggt
     1861 cgatgctctt cgtgctcgtg ctttcgacct tcatcggcct gggcgtcatc atacgggtgt
     1921 cgcgcttgtt gcacacgccc ttgatgtcgc tcaccaacgc catctccgcc atcgcggtgg
     1981 tcggatcgat cctcgtgacg ggcggcgagc accccaagga gatccgcatc ctgggagcgg
     2041 tggcgttgtt cgcatccatg accaacatcg tgagcggctt cctcatcacg gatcgcatgc
     2101 tgaagatgtt caagcagtcg ccagcagggg agaagaagtc gtgatccaca atatcgtcca
     2161 ggccgcctac ctggcggcga tggtgctctt catcttctcc ttgcacggga tgaatcaccc
     2221 gcgcaccgcg cgccgcggcg tgttctgcgg catcgcggcc atgctgctcg cggtggtcgc
     2281 gacgtggatg cagcccgaag tactcaatca cggctggatc gtggtggcga tcatcgccgg
     2341 cttcgtggtg ggcgtgccgc tctcgcgcgt gccgctgacc gcggtgcccc agcgaaccgc
     2401 gctctcgcac gccttcggcg gattggccgc cggcctggtg gggaccgcga agtattactt
     2461 gtgggtgggc gagagtccgg agcagttgac gaccttccgc atgaccgcga tcgtggccga
     2521 gatcatcctc ggcttcctaa cgttcaccgg cagcttgatg gcggcgggga agctccagga
     2581 gctcaagtgg atcccgcagc gccccgtcac ctaccggggc cagaacatcg tcaacatcgg
     2641 actgctggcg atcgcggtga tcgcgggtct ggtgctcatc gcgaatccga cgggccaata
     2701 cgcgcccttc ttgttcccgt tgatgatggg gctctcgctc ctgttcggcg tcctgctcgt
     2761 tattcccatc ggcggcgccg acatgccgac cgtgatctcg attctcaact cgtacgccgg
     2821 actctccgcg gtggcgatgg gcttcgttct cgacaacaaa ctgttgatca cagccggcgc
     2881 gctcgacggt tccagcggtt tgatcctgtc catcatcatg tgccgcgcga tgaaccggtc
     2941 gttcaccaat gtcctgttcg gcgcgttcgg ccaggtccag gccgcgaagg cgggcgcgga
     3001 gcagaaggtc tacaagcccg agaccatcga aggcgcggcg caagtgctcg agcaggccag
     3061 cctcgtggtc atcattcccg gctacggcat ggcggtggcg caggcccagc accgcgtgcg
     3121 cgaattgcac gaccagctga ccaagcgcgg gatcacggtc aagttcgcga tccaccccgt
     3181 ggcgggacgc atgccgggac acatgaacgt gctcctggcc gaagccgacg ttccctacgg
     3241 cgacctggtc gagatggacg agatcaacgg cgatatgccg cagtgcgacg tggccctcgt
     3301 ggtcggcgcc aacgacgtgg tcaatccggc cgcgcgcacc gacaagtcga gcccgatcta
     3361 cggcatgccg atcatcgacg ccgacaaggc caagaccgtg tacgcgatca agcgcagcaa
     3421 gaaccccggc ttcgccggca tcgacaacga gttgtacttc gccgaccaga ccttcatgtt
     3481 gttcggcgat gccaaggcgg tcgtgggcga gctggtcaag cagctcgcgc gcgagggcgc
     3541 cgccgcggcg cactagggat aatccggccg catggagttc ctggcgcgcg ttttcgattt
     3601 ctttctccac atcgacaagc acttgctcga ggtggtgacg acgtacggca cgtggacgta
     3661 cgccatcctg ttcgccatcg tgttcctcga gaccggcttc gtggtgacac cgttcctgcc
     3721 cggcgactcg ctcttgttcg cggcgggcgc cttggcgggc gcgggcgcgc tcgacgtggt
     3781 gtgggtgatg gtgctgctca ccatcgcggc catcttgggc gattcggtga actatgccat
     3841 cggacgcgcg ttcggcgtgc gcgcgttcac cgaaggaagc cggttcttca aggaggagta
     3901 cctcctgcgc acgcagcgct tctacgagaa gcacgggggc aagaccatcg tgctggcgcg
     3961 cttcgttccc atcgtgcgca ccttcgcgcc cttcgtggcg ggtgtggggc gcatgcgcta
     4021 cgtccgcttc tgggcctaca acgccgcggg gggcgtgctg tgggtcgtga gcctcaccct
     4081 gctcggctac ttcttcggca ccattccgtt cgtgaaggag aacttcacgg tggcgatcct
     4141 cgtcatcgtc ctgctctcgg tggtcccggt gttcgtggag atggcgaagc accggataca
     4201 gcgctctcgg gccgccgccg gcgaacagtc gtaagaacac ggtcgcgacc ccctggcggg
     4261 agcccctccc attcgttaga cttccctcac acgttgcccc ccgaacctcg gcgcgtgtgc
     4321 accgatccac ctactcggag gtttcaccat ggctgtaaaa ccgatccccg acggttatgc
     4381 caccgtcacc ccgtacctcg tcaagggagc gtcacgcgcg aggaaatgga ccgccgcgtg
     4441 cgcggcatga tgggggaatg agcatgagca ccaaccaacg gccccagaag ggcgaattca
     4501 acgagtacta cgccggctac atcgctaagg tgcccgacgg cgacatcgtc tcgatcctgc
     4561 gctcgcagat cgccgagtcg accacgatgc tcgagcgcgc gaacccggag cgcgtcgagt
     4621 atcgctacgc gcccggcaag tggacgctcc acgaagtggt gggccacgtg gtcgacatgg
     4681 agtgggtgtt cacggtgcgc g
//
LOCUS       DASPGK010000395         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1736975, whole genome shotgun sequence.
ACCESSION   DASPGK010000395 DASPGK010000000
VERSION     DASPGK010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736975"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VEC56_10925"
     CDS             complement(<1..987)
                     /locus_tag="VEC56_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV14705.1"
                     /translation="MTTTRTRRACVSAAAVVAALVLAPAAPVRGADPVLDAHTLVVGT
                     RAEPKSLNPVAITASEGHQIAGLVFLKLLEEQDDFLSFEPQLASSWTWSADSLAVTFH
                     LRPDALWSDGTPVTAHDVRFTWEVQVDTTVAWPNASIKSKIRDVEVKDARTVVFHFKE
                     RYLYQLMDANDGVILPKHVLGSVPRAELKTAPFGRAPVGNGPYALSRWESGQYLELSA
                     NPRYFGAAGGSAPQVQRVVFKFVPDAVTLLAQLKAGEIDLLEAVQPGDLASIRKSRPD
                     ATVHTVPSRRMSFIAWNEKRAPFDDRDVRRALTMAIDRAEIIRTVWQGYADEC"
     gene            complement(984..1742)
                     /locus_tag="VEC56_10930"
     CDS             complement(984..1742)
                     /locus_tag="VEC56_10930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV14706.1"
                     /translation="MSVPVSAVVLTQNNASIVARCIHSLAFADEIVVVDAESIDDTAE
                     IARASGARVVQHAWAGFAQQRRFGLSQARHEWVFVVDSDEEVTPELARAVAEAVRSDR
                     APVGYRIRRRNQFLGRWMEVGPWARDTQLRLFRKGAVSVIDASVHESYRVEGRVGVLS
                     APLNHYAHPTIAESVQRLNRYTTLEARDRASRRRVRALDPLVLPAGVFFKYYVAKGCW
                     RAGVEGFLLAAITAMYKAVLYVKIRSIQLAKARP"
     gene            complement(1739..>3020)
                     /locus_tag="VEC56_10935"
     CDS             complement(1739..>3020)
                     /locus_tag="VEC56_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV14707.1"
                     /translation="ERCHDLMKGLGIKHPPCAFASTREATIREANQLGYPVLLRPPYV
                     LGGRSMELVRGEDEILSCVDSALASSGSRALMIDKFIEGAVEVDVDCVSDGENVAIAG
                     IMEHMEEAGIHSGDSSCVLPPLALEDEIVEQIKEHTVRVARALRVIGLMNAQYAIKDN
                     KIYCFEINPRASRTVPYVSKATGVQWVKLATRVVMGEKLDMAKYGETIVPGYMSVKAP
                     VFPFDKFPGVDSILGPEMRSTGEVMGIGETFDEAFIKAQLAAGYRIPKGSHVFISVAN
                     RYKRKVIFPVKALRDMGYKMVATPGMARVLRSHGVPTREVHKISEGDYGLLEMIEDGT
                     IQLVVNMAWSKASIEDDKFIRLAANKMKVPCITTMAGFHALVLGLHTVTGGPVHVESL
                     QNYNARLHAPAATVSDADDAADAGAPPRAVGEAA"
BASE COUNT          469 a         1015 c         1034 g          502 t
ORIGIN      
        1 gcactcgtcc gcgtagccct gccacacggt gcgaatgatc tcggcgcgat cgatggccat
       61 cgtgagcgcg cgccggacgt cgcggtcgtc gaagggcgcg cgcttctcgt tccacgcgat
      121 gaagctcatg cgccgcgacg gcacggtgtg cacggtcgcg tcggggcggc tctttcggat
      181 cgacgcgagg tcgccgggct gcaccgcctc gagcaggtcg atctcgcccg ccttgagctg
      241 cgccaggagg gttaccgcgt cggggacgaa cttgaacacg acgcgctgga cctgcggcgc
      301 gcttccgccc gcggcgccga agtagcgcgg gttggccgag agctcgaggt actgtcccga
      361 ctcccagcgc gagagcgcgt agggtccgtt gcccacgggt gcgcgcccga agggcgccgt
      421 tttcaactcc gcgcgcggca ccgaacccag cacgtgcttg ggaaggatga cgccgtcgtt
      481 ggcgtccatc agttggtaca ggtatcgctc cttgaaatgg aacacgaccg tgcgtgcgtc
      541 cttgacctcc acgtcgcgga tcttggactt gatgctcgcg ttgggccacg cgaccgtggt
      601 gtcgacctgg acctcccacg tgaagcggac gtcgtgcgcg gtgacggggg tgccgtcgga
      661 ccagagcgcg tcggggcgga ggtggaacgt caccgcgagg ctgtcggcgc tccaggtcca
      721 cgacgacgcg agttgcggct cgaacgagag gaagtcgtcc tgctcttcga ggagcttgag
      781 gaacacgagg cccgcgatct ggtgtccctc ggaagcggtg atggcgaccg ggttcagact
      841 cttgggctcg gcgcgcgtgc ccaccaccag tgtgtgcgcg tccaacaccg ggtccgcgcc
      901 gcgcacgggt gctgcgggcg cgagcaccag cgcagccacc accgccgcgg cgctcacgca
      961 tgcgcgtcgc gttcgcgtcg tcgtcatggc cgcgccttcg cgagttggat cgaccgaatc
     1021 ttcacgtaga gaaccgcctt gtacatcgcc gtgatggcgg ccaggaggaa gccctccacc
     1081 ccggcccgcc agcagccctt ggcgacatag tacttgaaga acaccccggc gggtagcacc
     1141 agcgggtcga gcgcgcgcac gcggcggcgc gacgcgcgat cgcgcgcctc gagcgtcgtg
     1201 taccgattga gccgctgcac gctctcggcg atggtcgggt gagcatagtg attgagcggc
     1261 gccgatagaa cgccgacccg accctcgacg cgatagctct cgtgcaccga cgcgtcgatg
     1321 acactcaccg cgcccttgcg aaacaaccgc agctgcgtgt cgcgcgccca cggccccacc
     1381 tccatccagc gcccgaggaa ctggttgcgg cggcggatgc gatagccgac gggcgcgcgg
     1441 tccgagcgga cggcctcggc gaccgcgcgc gccagctcgg gtgtgacctc ttcgtcggag
     1501 tcgacgacga agacccactc gtggcgcgcc tgcgagaggc cgaaacgccg ctgctgcgca
     1561 aagcccgccc acgcgtgctg gaccacgcgc gcgcccgatg cgcgcgcgat ctccgcggtg
     1621 tcgtcgatgc tctccgcgtc gaccaccacg atttcgtcgg cgaacgcgag gctatggatg
     1681 cagcgcgcga cgatcgacgc gttgttctgg gtgaggacga ccgcagagac ggggacgctc
     1741 acgccgcctc gccgacggcc cgcggcggcg cgccggcgtc ggcggcgtcg tcggcatcgg
     1801 aaacggtcgc ggccggggcg tgcaggcgcg cgttgtagtt ttgtagcgac tcgacgtgca
     1861 cggggccgcc ggtgacggtg tgcaatccca gcaccagggc gtggaatccg gccatggtgg
     1921 tgatgcacgg gaccttcatc ttgttcgccg ccaggcggat gaacttgtca tcctcgatgg
     1981 aggccttgct ccacgccatg ttgaccacca gttggatggt cccgtcttcg atcatctcga
     2041 gcaggccgta gtcgccttcg ctgatcttgt gcacttcgcg cgtgggcacg ccgtgcgagc
     2101 gcagcacgcg cgccatgccc ggggtggcaa ccatcttgta gcccatgtcg cgcagcgcct
     2161 tgaccgggaa gatcaccttg cgcttgtagc ggttggcgac cgagatgaac acgtggctgc
     2221 ccttggggat gcggtagccc gcggcgagct gcgctttaat gaaggcctcg tcgaaggtct
     2281 cgccgatccc catcacctcg ccggtcgacc gcatctcggg gcccaggatc gaatcgacgc
     2341 cggggaactt gtcgaagggg aacacggggg ctttgaccga catatagccg gggacgatgg
     2401 tctcgccgta cttcgccatg tcgagtttct cgcccatcac gacgcgcgtc gcgagcttga
     2461 cccactgcac gccggtggcc ttgctcacgt acggcaccgt gcgcgaggcg cgcgggttga
     2521 tctcgaagca gtaaatcttg ttgtccttga tcgcgtactg cgcgttcatc aatccgatga
     2581 cgcgcagcgc ccgcgccacg cgcaccgtgt gctccttgat ctgctcgacg atctcgtcct
     2641 cgagggccag cggcgggagc acgcagctcg aatcgccgga gtgaatgccg gcctcttcca
     2701 tgtgctccat tatacccgcg atggcaacgt tctcaccgtc gctcacgcaa tcgacgtcga
     2761 cctcgaccgc gccctcgatg aacttgtcga tcatgagcgc gcgcgacccg ctcgatgcga
     2821 gcgccgagtc gacgcacgag agaatctcgt cctcgccgcg cacgagctcc atcgagcggc
     2881 cgcccaagac gtaaggcggg cgcaagagca ccgggtagcc gagctgattg gcttcgcgaa
     2941 tggtggcttc gcgcgtggac gcgaatgcgc acggggggtg cttgatgccc aggcccttca
     3001 tgaggtcgtg gcagcgctcg
//
LOCUS       DASPGK010000396         9098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_97459, whole genome shotgun sequence.
ACCESSION   DASPGK010000396 DASPGK010000000
VERSION     DASPGK010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97459"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..749)
                     /locus_tag="VEC56_10940"
     CDS             complement(207..749)
                     /locus_tag="VEC56_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14708.1"
                     /translation="MESRLRILVLALVTAAALGPAPAAAAKKAPPGDVASQSGSSDKG
                     EPSQGEVREAAGPMDVFADIERGWKAGQVDPILRHFGSQKVAISIQGTGPSGGTFSRN
                     QSYYLLKDLFRYTLTKKFEFVQYRKPNEDGNETFAVAERHYQRNDDSQLVKDKVYVSL
                     HLERDGKTERWVVDEIKSIR"
     gene            complement(885..1784)
                     /gene="secF"
                     /locus_tag="VEC56_10945"
     CDS             complement(885..1784)
                     /gene="secF"
                     /locus_tag="VEC56_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXV14709.1"
                     /translation="MEFFRTPDINFVGVARKALVVSGAVLIAGFISMGVQRGLKQSID
                     FAGGALVEVRTETAVPLQDIRSIVAAAGFENAEITRFGADNEFLIKVKSIEEADATAK
                     AIVAAIQNGIGQQQVDLRRVETVGPKIGAELRSSGVQAVIWSMIGILIYVGWRFDFRF
                     AVASILATLHDVLLTLTFFSFTGIEMSLGVLAAVLTIVGYSLNDTVVVFDRIRENLGL
                     KRGVEFGKLVNTSINETLSRTVITGTTTLLVLVSLIFLGGEVIRDFAITLFVGIIIGT
                     YSSVFVAAPIVLEWHRWRPLKSR"
     gene            complement(1794..3491)
                     /gene="secD"
                     /locus_tag="VEC56_10950"
     CDS             complement(1794..3491)
                     /gene="secD"
                     /locus_tag="VEC56_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXV14710.1"
                     /translation="MKIRWKLVVILAVVVWSVWALFPSYRYYSMTNDDRARLTSEQRD
                     QYTEKALKLGLDLQGGVHLVMEVDDSKLDENARRDVMDRAIKIYRNRIDQFGVSEPTI
                     QQQGDKRIIVELPGLEDVERAHRILGQTAQLEFRLVREPDEMNRVLQALDRNLRGVTV
                     TGSIIDTTGVKASDDTSHVHAPGEAPHTPSPSAVDSLLPTLATGEEGNVPLPEASADR
                     PFSSYLITMFDGGVVIDDRQMEVVDALLKTEQAKRAVPSNSAFLWGHEARPMQEGGDG
                     RILYLVEKNPTLDGSSLVDATTRPDPDDPSRLNVGFRLNRHGAIVFSRFTGENIGRRI
                     AIVLDAKVRSAPVVQSKIPSGEGRITGLDSDEEAGDLAIVLRAGALPAPFEIVEERTV
                     GPSLGRDSVEAGTRAALIGFILTLAFMVMYYKWSGALACTAMVLNLVIEMAILATLHA
                     TLTLPGVAGLILTIGMSVDANVLIFERIREELRRGKTVRAAIEAGYDRAFGTILDSNV
                     TTLITAFVLWQFGSGPIRGFATTLSIGILVSMFTALLCTRVVFDLVTTRRHVEKLSV"
     gene            complement(3684..4415)
                     /locus_tag="VEC56_10955"
     CDS             complement(3684..4415)
                     /locus_tag="VEC56_10955"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXV14711.1"
                     /translation="MMDKTFVRLGVNIDHVATLRQVRKALEPDPVAAAVAVEMAGADQ
                     ITVHLREDRRHIQNRDVRLIREVVRTELNLEMAATAEMQEIALALRPDAVCIVPEKRE
                     EITTEGGLNLVDASPSLGEMVATLKDADIDVTAFLDPDLRQVNEAARFRFDAVEMHTG
                     AYANAPRHRRDAELKKLKEAADAIRHLGMRVHAGHGLDYHNISPLLTVPHLEEVNIGH
                     AIVARAIMVGMDRAVRDMLHLLGRI"
     gene            complement(4412..5578)
                     /locus_tag="VEC56_10960"
     CDS             complement(4412..5578)
                     /locus_tag="VEC56_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXV14712.1"
                     /translation="MPRTTRNSNRDERAPKRAKLFVAALVVLALTGGAAVKFLQTTRG
                     AIFLVDHGVESALPRVEREVGATLKRSLEASGLRRQLRTQRGSGPVAWDLPCDESTDL
                     LLVNVALTEAARGIGAVVRTSEEADDGRTLLFHVGTHTRDTHRLTIRRLAPEAMAAQG
                     PAPRTPRIAIVIDDFGYTDGGIAREMLELEMPVTISILPQLRHSTDVLRLAKRSGRCV
                     LLHLPMEANQRYPEDLEPVTVDMSDAEIASLVGEYMESLPGVDGVNNHQGSRVTGDVR
                     VMRAVCRVLGDYDVFFLDSLTSPESVAYNVAIEAGLRAAANGGFIDDATDRRDDVETR
                     LRELADTAREKGVAIGIGHPHPWTLEALRAFESYLLTTDIELVSLCDLIESETP"
     gene            complement(5669..6298)
                     /locus_tag="VEC56_10965"
     CDS             complement(5669..6298)
                     /locus_tag="VEC56_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14713.1"
                     /translation="MKRVILLLLLLSACEDDPAQPQGNQRAQTFQALTTREAVLNNLE
                     LAWTKRNVDKIDELLDDDFTFYFSQGDVGGGLPPSWGRAAELAATTALFISNSSPPPS
                     GPVCTRVLVDIALDDAIWTEVPGPVAALGEVWYSATVSYTATFEMEPDDTYITVPGAT
                     AEFIVRQVEDDQWRLVEWHDLPGDAVGPAFAAAVGESTWGSIKYLYVAK"
     gene            complement(6316..6903)
                     /locus_tag="VEC56_10970"
     CDS             complement(6316..6903)
                     /locus_tag="VEC56_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14714.1"
                     /translation="MKRLAFLLLPILLLAACGDDPINPPIDDGNGGTSLTLQDLSEKW
                     HVLNNVEYAYAKRQSDVYDELLNADFTFFFAPGDVGGEIPAQWNRAEESSATRRLFQS
                     NAQSVPPADPVCRSIRIDLQFDKATMQWVEITPAAFPTETWYTTTVFYTFTFEMEPSD
                     TYIAQPGAKAEFTVRNTGTDQDPHWELVGFRDLGN"
     gene            complement(6989..7585)
                     /locus_tag="VEC56_10975"
     CDS             complement(6989..7585)
                     /locus_tag="VEC56_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14715.1"
                     /translation="MLKRSLMLCGLTAMALFLSSCLLDPDEDPPGDDPPPALELKPLT
                     EKWHVLNNIEYAYSRRVKSAYEELLNEDFTFFFAPGDVGGEIPATWDRNEEFGATGRL
                     FDSNGQPEPPADPVCRSIRLDLQYDPDQIQWVEKIPENFPAETWYYTTVFYTFTFEML
                     PSDTYIAQPGAKAEFAVRNAGTEQDPHWELVEFRDLGN"
     gene            complement(7718..8314)
                     /locus_tag="VEC56_10980"
     CDS             complement(7718..8314)
                     /locus_tag="VEC56_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14716.1"
                     /translation="MKRRITQAVLTCAITVAMSGCLFDTRDAEPPTDGSGGCTAIALD
                     VSNAVFEAMTCALESEQDAAYERVISERFVFSPTFDDSTDQTFAGTPVYDGWNKTVEL
                     DVLRLMLSDAQTLAVEFTPSILINQTTFVRYRVEYLLTVVNVATPGDTTQYGGVAQFD
                     VRNEGGNWRLTFWDEIETVPNLSTWGFLKGILRLRLNP"
     gene            complement(8324..>9098)
                     /locus_tag="VEC56_10985"
     CDS             complement(8324..>9098)
                     /locus_tag="VEC56_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14717.1"
                     /translation="YLDTEANPRDRDQVDTSVNLRLSSTPFTKFSANINLAYSASEFI
                     NIDASQSSNNRTRELYELRPAFTYVFNEMITVSQSYGVAIEYTDFVFTPTSNFLDRNL
                     IFTNRFDFKPTRHIGFVFDYGYNFHDNGSYLPDEVTGEEELSVQGEDRRDRINLRLDY
                     RVMSRTVQKAPSDPQLRQSLGVFAEQRYSRFEDRSVLSDSKTVTTDGQIVVGARGDYE
                     WGNGRTLKFTLARVKRSTKFGSEAEKNYWDMRSEFNYPF"
BASE COUNT         1599 a         2827 c         3013 g         1659 t
ORIGIN      
        1 ccacggccgc cgcgctggcg gccgccagca gcacggcgat cacgggaatc ggtacgcgct
       61 cgctccaggc cggcgaaagg aagtgccgga agaagatcgc gagccacgcg ccgatgagcg
      121 cgagcgcggg cagtatgatc atcaatcggg aagttcgcat cgaacgtagc atagcccacg
      181 ctcgaggtcg ggtcgagcgg ggtggttcag cggatcgact tgatctcgtc gaccacccag
      241 cgctcggttt tgccgtcgcg ttcgaggtgg agcgagacgt acaccttgtc cttgacgagc
      301 tgactatcgt cgttcctctg ataatgtcgc tccgcgacgg cgaaggtctc gttgccgtcc
      361 tcgttgggct tgcggtactg cacgaactcg aacttcttgg tgagcgtgta gcggaagaga
      421 tccttgagga gatagtagct ctggttgcgg gagaacgtgc cgcccgacgg ccccgtgccc
      481 tggatcgaga tcgcgacctt ctggcttccg aaatgacgaa ggatggggtc gacctggccg
      541 gccttccagc cgcgctcgat gtcggcgaag acgtccatgg ggccggcggc ttcgcgcacc
      601 tccccctgcg aaggctcgcc tttgtcggat gagccgctct gcgatgcgac gtcgccgggc
      661 ggggccttct tcgcggccgc ggccggggcc gggccgagcg cggcggcggt cacgagcgcg
      721 aggacgagta tgcgaaggcg cgattccatc ggcagaccga aatcatcggg aaagagcaat
      781 ccccatgccc cgaggttact cgttggggga aaacaagata cgatcacgac cggggggcgg
      841 cctgtcgccg gaaggggaca ccggcgtccc cgaagtggga cagactacct ggacttgagg
      901 ggccgccagc ggtgccactc gagcacgatg ggcgcggcga cgaagacgga cgagtaggtg
      961 ccgatgatga tccccacgaa cagggtgatc gcgaagtcgc ggatcacctc gcccccgagg
     1021 aagatgagac tcacgagcac gagcagcgtc gtggttcccg tgatcacggt gcgcgagagc
     1081 gtctcgttga tcgacgtgtt gaccagcttg ccgaactcca cgccgcgctt gagcccgagg
     1141 ttctcacgaa tgcggtcgaa caccaccacg gtgtcgttga gcgagtagcc cacgatggtc
     1201 agcaccgcgg ccagcacccc cagcgacatc tcgattcccg tgaacgaaaa gaacgtgagc
     1261 gtcaagagca cgtcgtgcag ggtcgcgaga atcgacgcca ccgcgaagcg gaaatcgaag
     1321 cgccagccga cgtagatcaa gatcccgatc atcgaccaga tgacggcctg cacgcccgag
     1381 ctgcgcagct cggcgccgat cttggggccc accgtctcga cgcgacgaag gtcgacttgc
     1441 tgctggccaa tcccgttctg gatcgcggcg acgatcgcct tcgcggtagc atcggcttct
     1501 tcgatgctct tcaccttgat caggaactcg ttgtcggccc cgaagcgcgt gatctccgcg
     1561 ttctcgaaac cggccgcggc cacgatcgag cgaatgtcct ggagcgggac ggccgtctcg
     1621 gtgcgcactt cgaccagcgc gccgcccgcg aagtcgatcg actgcttcaa gccgcgctgg
     1681 acgcccatcg agatgaagcc ggcaattaga acggcgccgg acacgaccag cgccttgcgt
     1741 gcgacgccga cgaagttgat atcgggtgtg cgaaagaatt ccatgtctgt ctcctagacg
     1801 ctcaacttct cgacgtggcg tcgggtcgtt acgaggtcga acacaacgcg cgtgcacagg
     1861 agcgcggtga acatactgac caggatgccg atactcaatg tggtggcgaa gccgcgaatc
     1921 ggtcccgagc cgaactgcca caagacgaac gcggtgatca gcgtcgtcac gttggagtcc
     1981 aagatggtgc cgaaggcgcg gtcgtagccg gcctcgatgg cggcgcgcac ggtcttgccg
     2041 cggcgcagct cttcgcggat gcgctcgaag atgagcacgt tggcgtcgac cgacataccg
     2101 atggtgagga tcaagccggc cacgccgggc agggtcaagg tcgcgtgcag cgtggcgagg
     2161 atcgccattt cgatgaccag gttcagcacc atggccgtgc aggccagcgc acccgaccat
     2221 ttgtagtaca tcaccatgaa ggcaagggtg agaatgaagc cgatcaacgc ggcgcgcgtc
     2281 ccggcttcga ccgaatcgcg gccgagcgac gggcccactg tgcgctcctc gacgatctcg
     2341 aagggcgcgg ggagtgcgcc cgcgcgcagc acgatggcca agtcgccggc ctcttcgtcg
     2401 ctgtccaggc cggtgatgcg gccttcgccg ctcgggatct tgctctgcac gaccggcgcc
     2461 gagcgcacct tggcgtccaa tacgatggcg attcgtcgac cgatgttctc tcccgtgaac
     2521 cgcgagaaca cgatggcgcc gtgacgattc agccggaatc caacgttcag gcggctgggg
     2581 tcgtcggggt cggggcgcgt ggtcgcgtcc accagcgagc tgccgtcgag ggtgggattc
     2641 ttctcgacca gatagaggat gcgaccgtcg ccgccctcct gcatcgggcg cgcctcgtgt
     2701 ccccacagga acgccgagtt ggacgggacc gcgcgcttgg cctgttcggt cttgagcagc
     2761 gcgtcgacca cttccatctg gcggtcgtcg atcaccacac cgccgtcgaa catggtgatc
     2821 aaatacgagc tgaagggccg gtctgcggat gcctcgggga gcggcacgtt gccctcttca
     2881 ccggtcgcca gcgtgggcag cagcgaatcg acggcggagg gagacggcgt gtggggcgcc
     2941 tcgccgggcg cgtgcacgtg cgacgtgtca tcggaggcct tcaccccggt cgtgtcgata
     3001 atcgacccgg ttacggtgac gccccgcagg ttgcgatcga gtgcctgcag cacgcgattc
     3061 atctcgtcgg gctcgcgcac gagccggaac tcgagctgcg cggtctgtcc caggatgcgg
     3121 tgcgcgcgct cgacgtcctc caggccgggc agctcgacga tgatgcgctt gtcgccctgc
     3181 tgctggatgg tcggctcgga gacgccgaac tggtcgattc ggttacgata gatcttaatg
     3241 gcccggtcca tcacgtcgcg gcgggcgttc tcgtcgagct tggagtcgtc gacttccatg
     3301 acgaggtgga cgccgccctg gaggtccaag ccgagcttga gtgctttctc ggtgtactgg
     3361 tcgcgctgct cgctggtcag gcgggcgcgg tcgtcgttgg tcatcgagta gtagcggtag
     3421 ctcgggaaga gagcccacac gctccacacc acgacggcga ggatgaccac cagcttccaa
     3481 cggatcttca tcgatacccc taaagagcga gagtccagcc aggcccgggc tcggccccgc
     3541 ggcactggtt tgttccggag agttgggcgc ccgtagtccc taacgaagaa ttcattcgga
     3601 gggcgttttg gccggaaata aggggggatt atacacgacc ccggggcccc gtcaacgggc
     3661 gactttggga gcgcggcccg gccttagatt cggcccagca ggtgcagcat gtcgcggacg
     3721 gcgcggtcca tcccgaccat gatcgcccga gcgacgatcg cgtggccgat attcacctct
     3781 tcgaggtgcg ggaccgtgag cagcggcgag atgttgtggt agtcgaggcc gtggccggcg
     3841 tgcacgcgca tgccgaggtg ccgaatcgcg tcggcggcct ccttgagttt cttcagctcc
     3901 gcgtcgcggc ggtgccgggg cgcgttggcg tacgcgcccg tgtgcatctc gaccgcgtcg
     3961 aagcggaagc gcgccgcttc gttgacttgc cgcagatcgg ggtcgaggaa cgcggtcacg
     4021 tcgatgtcgg cgtccttcag cgtggccacc atctcgccca ggctcgggct cgcgtcgacc
     4081 aggttcaaac cgccttcggt ggtgatttcc tcgcgcttct cggggacgat gcagaccgcg
     4141 tcgggccgca aggccagcgc gatctcctgc atctccgcgg tcgccgccat ctcgaggttg
     4201 agctcggtgc gcacgacttc gcgaatcagc cgcacgtcgc ggttctggat gtggcgacga
     4261 tcttcgcgca gatggacggt aatctggtcg gcgcccgcca tctcgaccgc caccgcggcc
     4321 gcgaccgggt cgggctcgag cgccttgcgc acctggcgca gtgtcgccac gtggtcgatg
     4381 ttgactccca gccgaacgaa ggtcttgtcc atcatggcgt ctccgattcg atcaagtcgc
     4441 agagcgaaac cagctcgatg tccgtggtga gcaggtacga ttcgaacgcg cgcagcgcct
     4501 cgagcgtcca cgggtgcggg tgcccgatgc cgatcgcgac tcccttttcg cgcgcggtat
     4561 cggccagctc gcgcagccgc gtctcgacgt cgtcgcggcg gtcggtggcg tcgtcgatga
     4621 agccgccgtt cgccgccgcg cgcaggcccg cttcgatcgc cacattatag gcgacggatt
     4681 ccggcgaagt caaagaatcg aggaagaaga cgtcgtaatc gccgagcacg cggcagaccg
     4741 cgcgcatgac gcgcacgtcg ccggtcacgc gcgatccctg gtggttgttg accccgtcga
     4801 cgcccggcag gctctccatg tactcgccca ccagcgacgc gatctcggcg tcgctcatgt
     4861 cgacggtgac gggctcgagg tcctcggggt agcgctgatt cgcctccatg ggaagatgca
     4921 ggaggacgca gcgcccgctg cgcttggcga gtcgcagcac gtcggtcgaa tggcggagct
     4981 gcgggagaat ggagatcgtg accggcatct cgagctcgag catctcgcgc gcgatgccgc
     5041 cgtcggtgta gccgaagtcg tcgatcacga tcgcgatcct gggtgtgcgg ggcgccggtc
     5101 cctgcgccgc catcgcctcg ggtgccaggc gccggatggt gaggcggtgc gtgtcgcgcg
     5161 tatgggtgcc cacgtgaaaa aggagcgtgc ggccgtcgtc ggcctcttcg ctcgtgcgca
     5221 cgaccgcgcc gatcccgcgc gccgcttcgg tgagcgcgac gttgaccaag agaagatcgg
     5281 tgctctcgtc gcacggaagg tcccacgcga ccgggccgct tcccctctgc gtgcgcagct
     5341 ggcggcgaag cccgctcgcc tccagcgatc gcttgagcgt cgcgccgact tctctttcga
     5401 cgcgcggaag cgcgctctcg accccgtgat cgaccaggaa gatcgcgccg cgcgtggtct
     5461 ggaggaactt gaccgccgca ccgccggtga gcgccagcac caccagcgcc gcgacgaaca
     5521 gcttcgcgcg cttgggcgcg cgttcgtctc tattggaatt tcgagtggtg cgcggcatgg
     5581 ggttgtggcc gcgcggagac tatcggcggg cggaagaaat cgtcaagctc gggctaagga
     5641 tcgaggacga ctatgcgagc ggacgacttc attttgcgac gtatagatac ttgatgcttc
     5701 cccaagtaga ttcccctacg gccgcggcga acgcgggacc aacggcgtca cccggcaggt
     5761 cgtgccactc gaccaggcgc cactggtcgt cttcgacctg ccgcacgatg aactccgcgg
     5821 tggctccggg gacggtgatg tacgtgtcgt ccggttccat ctcgaacgtt gccgtgtagg
     5881 aaacggtcgc cgagtaccaa acctctccga gtgccgctac gggacccggc acttccgtcc
     5941 agatcgcgtc gtcgagcgcg atgtcgacga ggacccgggt gcaaaccggc ccgctcggag
     6001 gcggagacga gttcgatatg aagagtgcgg tggtggcggc caactctgcg gcgcggcccc
     6061 acgaaggagg aaggccaccg ccgacgtcac cctgcgaaaa atagaaggtg aagtcatcgt
     6121 cgagaagctc gtcgatcttg tcgacgttgc gcttcgtcca ggcgagctcg aggttgttca
     6181 ggacggcttc gcgcgtggtg agggcctgga acgtctgcgc acgttggttg ccttgcggct
     6241 gggcggggtc gtcttcgcag gcggacagaa gcaagagcaa caagatgaca cgcttcatcg
     6301 aagctcccac cggagctaat tgccgaggtc gcggaacccg accagctccc agtggggatc
     6361 ctggtcggtg ccggtgttgc gcactgtgaa ctccgccttc gcccccggct gcgcgatgta
     6421 ggtatcgctc ggttccatct cgaacgtgaa cgtgtagaag actgtggtcg tgtaccacgt
     6481 ctcggtcgga aaagcggccg gcgttatctc cacccactgc atggtggcct tgtcgaactg
     6541 caggtctatc cgaatggacc ggcacacggg gtcggcaggg ggaacggatt gagcgttgga
     6601 ttgaaacagc cggcgggttg cgctcgactc ttcggcgcga ttccattgcg cggggatctc
     6661 gccgcccacg tctccgggcg cgaagaagaa ggtgaagtcg gcgttgagca gctcgtcgta
     6721 gacgtcggac tggcgcttcg cgtacgcgta ctcgacgttg ttcagcacgt gccacttctc
     6781 gctcaagtcc tgcagagtca gggacgtgcc tccgttgccg tcatcgatcg gaggattgat
     6841 ggggtcgtcc ccgcaggcgg cgagcagaag gatcggcaga aggagaaaag cgagtcgttt
     6901 catggttctc caatcgcccg caatcatagc acgaaacgaa aagaggcacc ggcttggccg
     6961 gtgcctcttc gtgtgatgcg tcgcgaggct agttgcccag atcgcggaac tcgacaagct
     7021 cccagtgtgg gtcctgctcc gtgccggcgt ttcgcaccgc gaactcggcc tttgcgcccg
     7081 gctgcgcgat atacgtgtcg ctcggcagca tttcgaacgt gaacgtgtag aacaccgtcg
     7141 tgtagtacca cgtctcggcg gggaagttct cgggaatctt ctcgacccat tgtatctggt
     7201 cggggtcgta ctggagatcc agacggatgg accggcagac tgggtccgcc ggaggctcgg
     7261 gctgcccgtt cgaatcgaac agtctgcccg tcgccccgaa ctcctcgttg cgatcccacg
     7321 ttgcgggaat ctcgccgccc acgtcgccgg gcgcaaagaa gaaggtgaag tcctcgttga
     7381 gcagctcttc ataggccgac ttcacgcgcc gtgaataggc gtactcgata ttgttcagga
     7441 cgtgccactt ctccgtgagg ggcttgagct caagagccgg gggtggatcg tcgccgggcg
     7501 ggtcctcgtc cgggtccaag agacacgaag acaagaacaa agccatggcc gtgagcccgc
     7561 agagcatcaa actacgcttc aacatcgctg cagatccttt ccgcgagtcg tcgtcgcaca
     7621 agagcaggtg aatcatacac caaccacaat cataacgtca agattcataa tgagttaatt
     7681 catgtcctag gctaccgaat cactacccgt cgttccgtta cgggttcagt cgtaacctca
     7741 agattccctt gaggaatccc cacgtggata ggtttggaac ggtctcgatc tcgtcccaga
     7801 aagtcagacg ccagttgccg ccctcgttgc ggacatcgaa ctgcgccacg cccccgtact
     7861 gcgtcgtgtc cccgggtgtc gccacgttga ccacggtcag caagtactcg acccggtagc
     7921 gcacgaaggt cgtctggttg atcagaatcg aaggggtgaa ctcgaccgcc agggtctggg
     7981 cgtccgagag catcagcctc agcacgtcca actcgaccgt cttgttccag ccgtcgtaca
     8041 cgggtgtccc ggcgaacgtt tgatcggtcg agtcgtcgaa tgtgggcgag aagacgaagc
     8101 gctcgctgat gacgcgttca taggctgcgt cctgctcgct ctcgagcgcg cacgtcatcg
     8161 cctcgaacac ggcgttggag acgtcgagag cgatcgcggt gcatccgccg ctgccgtcgg
     8221 tggggggctc ggcgtcacgc gtgtcgaaca gacaccccga catcgcgacc gtgatcgcgc
     8281 acgtcaatac ggcctgagtg attcttctct tcatgacttc catctagaag gggtagttga
     8341 actccgaacg catgtcccag tagttcttct ccgcctccga gccgaattta gtggagcgct
     8401 tgacccgcgc cagcgtgaac ttgagcgtcc ggccgttgcc ccattcgtag tccccgcggg
     8461 cccctaccac gatctgcccg tcggtcgtga ccgtcttgga gtccgacaac accgatcggt
     8521 cctcgaaccg gctgtaccgc tgttccgcga atacgcccag cgactggcgc agctgcggat
     8581 cgctgggtgc cttctgcacg gtgcgcgaca tcacccggta gtcgagccgc agattgatgc
     8641 gatcccgtcg gtcctcgccc tgcacggaaa gctcttcctc acccgtgacc tcgtccggca
     8701 ggtacgaccc gttgtcgtgg aagttatagc cgtagtcgaa cacgaatccg atatggcgcg
     8761 ttggcttgaa atcgaaccga ttcgtgaaga tgaggttgcg atccagaaag ttgctggtcg
     8821 gcgtgaagac gaagtccgtg tactcgatgg cgactccgta cgactggctc accgtgatca
     8881 tctcgttgaa gacataggtg aacgccgggc gcagctcgta gagctcgcgc gtgcggttat
     8941 tgctcgactg gctggcgtcg atattgatga actcggaggc cgagtacgcg aggttgatgt
     9001 tcgcgctgaa tttcgtgaag ggcgtggaac tcaagcgtag attgacgctg gtgtcgacct
     9061 gatcgcggtc gcgggggttg gcttcggtgt cgagatac
//
LOCUS       DASPGK010000397         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1620288, whole genome shotgun sequence.
ACCESSION   DASPGK010000397 DASPGK010000000
VERSION     DASPGK010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620288"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VEC56_10990"
     CDS             complement(<1..252)
                     /locus_tag="VEC56_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14718.1"
                     /translation="MKHTRPDHGLDSHPSGSAFPATRHSLLSLARSGDHATRHRAHDA
                     LFSCYWKPVYKYIRLRWNQRAEDAEDLTQGFFAGAIDSSF"
     gene            438..>2234
                     /locus_tag="VEC56_10995"
     CDS             438..>2234
                     /locus_tag="VEC56_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV14719.1"
                     /translation="MRPSIRNGFRRLAAVPAALAVVAVAGFGTGASAQRSTDVATLKE
                     LSQKFERSLQAKQTPLYFNLLASTDPAQQALNVNPEIKLMYIRENGVPAYYTVHNLNA
                     ALTTRTYDVWPVGVGNGFYNLTGSTTAAGELAVWDGGAVRAAHQEFGGRVTVMDGGSL
                     LAHSTHVAGTMVGAGINVSARGGSYAAALHSYEWTNDTIEMAAAAAAGLQVSNHSYGF
                     ITGWFLNGATWYWYGDLAVSASEDYGFGYYDGSALEYDEIAYDAPYYLICVAAGNERD
                     DNGPGAANHQHWDNGLGQWVAAVDFHPADGQAGGYDTISYFSTAKNILCVGACNDFPS
                     GYTQPSDITLTAFTSWGPTDDGRIKPDIVANGAGLFSCTSTANNAYTTMSGTSMATPN
                     AAGSVNLIAQDYEVVKGASPWSSTLKALVINAADETGPADGPDYMNGWGLLNTHRMAD
                     IVHAAPGADLGVLEAGLTDGETDTYYFEITSPQDARVTIVWTDPEGSPSPIALDNPTP
                     KLINDLDLRVVHVPTATTTLPWRLDRLAPANAATKADNVVDNVEQVDIDTAPAGLYRV
                     TVTHKGSLDPAGAQNYSLVYRGMHEVQSTPVGG"
BASE COUNT          407 a          748 c          742 g          337 t
ORIGIN      
        1 gaagctagaa tcgatcgcgc cggcgaagaa cccctgggtc agatcttcgg cgtcttcggc
       61 gcgttggttc caccgcaggc ggatgtactt gtagaccggc ttccagtagc acgaaaacag
      121 cgcgtcgtgc gcgcggtgcc gggtcgcgtg gtcgccactc cgggccagcg acaggagcga
      181 atgccgggtc gcgggaaagg cggacccgga tgggtgcgaa tcgaggccgt gatcggggcg
      241 ggtatgcttc atgcgaggtc tcattatagc agtgcgcggg gtggcgcgcg gttgcgcgtg
      301 attccctaat cgaaccgggc ggcgtggcct gtgggcgccg actctgcgcg ggcgcccagg
      361 caaccgctgt gttataattg aggcgctccg tgcgtcccag tcagcaacgt ccacctctag
      421 gaaacaggag agactcgatg cgtccatcca tccgtaacgg cttccgtcgt ctcgcggccg
      481 tgcccgcggc gttggccgtg gtcgcggtcg cgggattcgg caccggcgcg tccgcccagc
      541 ggtccaccga cgtcgccacg ttgaaagagc tgtcgcaaaa gttcgaacgg agcctgcaag
      601 cgaagcagac gcccctgtat ttcaacctgc tcgcgtcgac cgaccccgcg cagcaggcgc
      661 tcaacgtcaa tcccgagatc aagctgatgt acatccgcga gaacggggtg ccggcgtact
      721 acacggtgca caacctgaac gcggcgctga cgacgcgcac ctacgacgtg tggccggtgg
      781 gggtcggaaa cggcttctat aacttgaccg gctcgaccac ggcggcgggc gaactggcgg
      841 tgtgggatgg cggcgcggtg cgcgcggcgc accaggaatt cggcggccgc gtgaccgtca
      901 tggacggcgg aagcctcctt gcacactcga cgcacgtggc gggtaccatg gtcggggccg
      961 gcatcaacgt cagcgcgcgc ggcgggtcgt atgcagcggc tctacactcc tacgagtgga
     1021 ccaacgatac catcgagatg gccgcggcgg ccgccgccgg gctgcaggtg tcgaaccact
     1081 cgtacgggtt catcaccggc tggttcttga acggggcgac gtggtattgg tacggcgacc
     1141 tcgccgtcag cgccagcgaa gactacgggt tcggatacta cgacgggagc gccctcgagt
     1201 acgacgagat cgcgtatgac gccccgtact acttgatttg cgtcgccgcc ggcaacgagc
     1261 gcgacgacaa cggacccggc gcggccaacc accagcactg ggacaatggc ctcggccagt
     1321 gggtcgcggc cgtcgacttt catcccgccg acggacaggc ggggggctac gacaccatca
     1381 gctacttcag cacggccaaa aacatcctgt gcgtgggcgc gtgcaacgac ttcccgagcg
     1441 ggtacacgca gcccagcgac atcacgctga ctgcctttac cagctggggc cccaccgacg
     1501 acggccgcat caagcccgac atcgtggcca acggcgccgg actgttctcg tgcaccagca
     1561 cggcgaacaa tgcctacacc accatgtcgg gcacgtcgat ggcgacaccc aacgcggccg
     1621 gctcggtcaa tttgatcgcg caggactacg aagtcgtcaa gggcgcgtcg ccgtggtcgt
     1681 cgacgttgaa ggcgctcgtg atcaacgcgg ccgacgaaac cggccccgcc gacggccccg
     1741 actacatgaa cgggtggggg ctgctcaaca cgcaccgcat ggcggacatc gtgcacgccg
     1801 ccccgggcgc cgacctcggc gttctcgaag cgggtttgac cgacggagag accgacacct
     1861 actacttcga gatcacttcg ccgcaggatg cgcgcgtgac catcgtgtgg acggaccccg
     1921 aggggtcgcc ctcgccgatc gcgctcgaca acccgacgcc caagctcatc aacgacctcg
     1981 acctccgcgt cgtgcacgtg ccgactgcga ccaccacgct cccgtggagg ctggatcgcc
     2041 tcgcgccggc gaacgccgcc accaaggccg acaacgtggt cgacaacgtc gaacaggtcg
     2101 acatcgacac cgcgcccgcg ggactctatc gcgtcacggt gacccacaag ggctcgctcg
     2161 acccggcggg tgcgcagaac tattcgctgg tgtatcgcgg catgcacgaa gtccagtcga
     2221 cgccggtcgg cgga
//
LOCUS       DASPGK010000398         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1620304, whole genome shotgun sequence.
ACCESSION   DASPGK010000398 DASPGK010000000
VERSION     DASPGK010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620304"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="VEC56_11000"
     CDS             <1..2116
                     /locus_tag="VEC56_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV14720.1"
                     /translation="AVAGTTRGAIADPQGHFVVPELPAGTYALVVSRVGHETLRTADF
                     VVMAGDTTRVDVALVPALIEANPVVVTAARTEQTARMAAASVSVLNREEIAARDPVTF
                     DQAVESVPGLNAFRSTGGISVQSMSIRGSSDVAGGGVGNRVLLLVDGRPALTSDSGGA
                     FWSLVPVQFVDRVEVVKGAFSSLYGSTAMGGVINVITRRPGPVSSGKLEMKLGFYEQP
                     PADIRYTDETLYQSEVTADYSARLGRTSFLVSATRKESDGYAENTAFEFYDVYGKVLY
                     DVSSERKLELTLGGGQADNDYPHAWLNSAQPLQVRDAYTDDLQEKTYGSADLWYWGLS
                     GEDMKYSMRAYYYHHEQRSFFNEDDPNQAIPGNEPYGLRTSIDGDKIGNLLQLDWRLH
                     ERHLLVAGSDIQIDYVHSSPDTVMYGDHQINNYSVFLQDDVEIARWLSATVGGRYDWN
                     HLVGVRTLEQFSPKFGLVWTPDGEVSVRALYGQAFRAPTIAEMFLQKELGGGVEFLPN
                     PDLEPEHLTLSIEAGVRWSPSALFDFDGAVFRYEYEDMIFWEDISAETGIPALYQVRN
                     LNRASMQGVETSLHFRWRALTATASYTYLDARDESDDRDDRTLPYRPEHSAAIGADLG
                     WRRWMLHGDARYRSRIDEVFLYPLQAPDAFWVFNAAAQCRLNGAWTFSIKVNNLLDTP
                     FEELARYRMPGRNWMFGTAMHF"
BASE COUNT          376 a          703 c          690 g          369 t
ORIGIN      
        1 cgccgtcgcc ggcacgacgc gaggtgccat cgccgacccg cagggacact tcgtcgtccc
       61 cgaactgccg gcgggaacct acgcactcgt cgtctcgcgc gtgggccacg agaccttgcg
      121 caccgccgac ttcgtcgtca tggccggcga caccacccgc gtcgacgtcg ccctcgtgcc
      181 cgcgttgatc gaagccaatc ccgtcgtcgt aaccgccgcg cgcaccgagc agacggcgcg
      241 catggcggcc gccagcgtgt cggtcctcaa tcgggaggag atcgccgccc gtgacccggt
      301 gacgtttgat caggcggtgg agtccgtgcc cgggctcaac gcgtttcgat cgaccggtgg
      361 catatcggtg cagtcgatgt cgatacgcgg cagctccgac gtcgccggcg gcggcgtggg
      421 caatcgcgtc ctcctcttgg tcgacgggcg cccggcgctg acttcggatt ccgggggcgc
      481 gttctggagt ctcgtccccg tgcagttcgt cgaccgcgtc gaagtggtca agggcgcgtt
      541 ctcgagcttg tatgggtcga ctgccatggg cggcgtgatc aacgtcatca cgcgccggcc
      601 cgggcccgtc tcctcgggca agctcgagat gaagctcgga ttctacgagc agccaccggc
      661 cgacattcgc tacaccgacg agactctgta ccagagcgag gtcaccgccg actattccgc
      721 gcgattgggc cgaaccagct tcctggtcag cgcgacgcgc aaggagtctg acggctacgc
      781 ggagaacacc gcgttcgagt tctacgacgt gtacgggaag gtcctctacg acgtcagcag
      841 cgagcgcaag ctggagctca cactcggggg cggccaggcc gacaacgact acccgcacgc
      901 gtggttgaac tccgcgcagc cattgcaggt tcgcgacgcc tacaccgacg atcttcagga
      961 gaagacgtac gggagcgccg acctgtggta ctggggcctc agcggagagg acatgaagta
     1021 ctcgatgcgc gcgtactact atcaccacga acagcgctcg ttcttcaacg aagacgatcc
     1081 gaaccaggct atcccgggca acgagccgta cggcctgcgc accagcatcg atggcgacaa
     1141 gatcggcaat ctccttcagc tcgactggcg attgcacgag cgtcacctgc tggtggccgg
     1201 ttccgacatc cagatcgact acgtccactc gtcgccggac acggtcatgt acggcgacca
     1261 tcagatcaac aactactcgg tgtttctgca ggacgacgtc gagatcgcgc gctggctgtc
     1321 ggcgaccgtc ggcggccgct acgactggaa ccacctggtg ggggtgcgca cgctcgagca
     1381 gttcagcccc aagttcggtt tggtatggac gccggacggc gaggtctcgg tgcgggcgct
     1441 ctacggacag gccttccgcg cccccacgat cgcggagatg ttcttgcaga aggagctcgg
     1501 cggcggggtc gagttccttc ccaatcccga tctcgaaccc gagcacttga cgctctcgat
     1561 cgaggccggg gttcgctgga gcccgtcagc cctcttcgac ttcgacggcg ccgtgtttcg
     1621 ctacgagtac gaggatatga tcttctggga agacatctcc gcggagacgg gcattcccgc
     1681 gctgtaccaa gtgcgcaacc tgaaccgcgc gagtatgcag ggtgtggaga cgagcctgca
     1741 ctttcgctgg cgcgcactga ccgcgacggc gagctacacc tacctcgatg cgcgcgacga
     1801 gtcggacgat cgtgacgacc gcacgctgcc gtatcgcccc gagcactcag cggcgatcgg
     1861 cgccgacctc ggatggcggc ggtggatgct ccacggagac gcgcgctacc gcagccggat
     1921 agacgaagta ttcttgtatc cgctgcaggc gccggacgcg ttctgggttt tcaacgcggc
     1981 cgcgcagtgc cggctgaacg gtgcgtggac gttttcgatc aaagtgaaca atctcctcga
     2041 tacgccattc gaagagctgg cccgctaccg tatgccgggg cgcaattgga tgttcggcac
     2101 cgcgatgcac ttttaaggag gccccgacgt gaccgaga
//
LOCUS       DASPGK010000399         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1033268, whole genome shotgun sequence.
ACCESSION   DASPGK010000399 DASPGK010000000
VERSION     DASPGK010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033268"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VEC56_11005"
     CDS             complement(<1..613)
                     /locus_tag="VEC56_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14721.1"
                     /translation="MTARDQKRRLCVVGGMFGLAVLVLWVRLVQVQYVRHDEFRSIAE
                     KQRVAPRVVAADRGGIFDREGRPLAMNIRRSSVAIQPARVSDADAVARALSRTLGVSV
                     RSVRDKLRSRKSFVYVKHDVVLPEATRRELAKLDGVVVELKASRIYPYDAVGSKVIGF
                     VSRENGGLSGVEAAYDRALRGSDGRETVVRNGAYKSDRYYTQIE"
     gene            complement(610..1038)
                     /locus_tag="VEC56_11010"
     CDS             complement(610..1038)
                     /locus_tag="VEC56_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14722.1"
                     /translation="MKRRNRMLVAGQFLLDALAGKLGAPMMVMAYATFAVLLLGYVST
                     QVYTSSLMEDVAARKREELVLRERIGVLTARYATMTSKSRVSRHCEESLGLVGATQSD
                     VVRVAVDGPVPRDAARPGAPVRVDQVLGTGVSELSQVMRR"
     gene            complement(1035..2039)
                     /gene="rsmH"
                     /locus_tag="VEC56_11015"
     CDS             complement(1035..2039)
                     /gene="rsmH"
                     /locus_tag="VEC56_11015"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXV14723.1"
                     /translation="MTMVVVPFGWSGAQARKDNAMQHLPVMVDEVVRYLLHERTRVVM
                     DGTVGFGGHAEAILSARPDIRLIGVDRDPAALEAAAVRLRGYADRVHLVRGLYSDFEH
                     ALAGVGKVDGVLLDLGFSSPQLDDPARGFAHSAHGPLDMRMDGEGETAALMLARLDVD
                     QIGTLLRELGEVRQARRVARAIASAAAAGTLSTTSDLRAAVIAALGRGVAPAELSRVF
                     QAVRIAINRELDALRAFLDGVLDVLNPGGRLVVLSYHSLEDRMVKTFLRDAAATCVCP
                     PNVPVCVCGRVPRLEVLTRKGVRASHDEIAANPRARSATLRAAAKVSNGARVSGGRVQ
                     "
     gene            complement(2049..2492)
                     /locus_tag="VEC56_11020"
     CDS             complement(2049..2492)
                     /locus_tag="VEC56_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14724.1"
                     /translation="MQGTFGEKHVHGIDPKGRLQLARPVRELLKLKKGDRLHLLPNIE
                     DPPFLEVRTTAQWDEYERRFLSQAPGDLKRDFVRFVQLHRETVVADAQGRLILPKSLR
                     DRCQLESTVVVIHMSSCVEVWSPKHIERRYAEMAKAFKHINNTFF"
     gene            complement(2671..3480)
                     /locus_tag="VEC56_11025"
     CDS             complement(2671..3480)
                     /locus_tag="VEC56_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV14725.1"
                     /translation="MTPVLPDIAVPPDLARADMPDLLRHWYLGTRARRHMMMRRFMEV
                     DRHLDLAAGRRVLDVGSAWGFNVMALNALGFHAVGVDIVPDPFVVGKRIADYNRIPFD
                     VAGADARALPFGDARFDHVTLVETFEHIFAEDRPRAMAECFRVLRPGGRIVLSTPNHA
                     SLVERAKRVAVRHPWIRAKLPSMCYPDLGSTRDDYHPYRYHRPLPDREIVSLLEGAGF
                     RVTEVSHFLFMLKSTPDAWYAPWTVLEKTSERTPGVRGLAATVCVVASKPS"
     gene            complement(3477..>3916)
                     /locus_tag="VEC56_11030"
     CDS             complement(3477..>3916)
                     /locus_tag="VEC56_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14726.1"
                     /translation="LYVRVVLPPSRAFSADLTGPMTELALYLRDNSPPDAVVAAADIG
                     YLAFYSQRRVLDLGGLVEPETGKLREAHDYEDIVARGLYFDVPGYPHVDYFVDRDHQA
                     DRFDGGIVGGRRFERVYATEVRNLGIRKPGVFHYVLYRLTVVQ"
BASE COUNT          684 a         1333 c         1310 g          589 t
ORIGIN      
        1 gttcgatctg cgtgtagtag cggtcgctct tgtaggcgcc gttgcgcacc accgtctcgc
       61 gcccgtcgct cccgcgcagg gcgcggtcgt acgcggcctc gaccccggac aggccgccgt
      121 tttcgcggct cacaaagccg atcaccttgc tgccgaccgc gtcgtacgga tagatgcggc
      181 tggccttcaa ctccacgacc acaccgtcga gcttggccaa ctcgcgacgc gtcgcttccg
      241 gcagcacgac gtcgtgcttg acgtagacga atgacttgcg cgagcgcagc ttgtcgcgca
      301 cggatcgcac cgagacgccc agcgtgcgcg agagggcgcg cgcgaccgcg tcggcatcgc
      361 tgacgcgtgc gggctggatc gccaccgacg agcgacggat gttcatggcc agcggacgtc
      421 cctcgcggtc gaagatcccg ccgcggtcgg ccgcgaccac gcgcggcgcc acgcgctgct
      481 tctccgcgat gctccggaat tcgtcgtgac ggacgtactg gacctggacc agacgcaccc
      541 acagcaccag cacggcgagc ccgaacatcc cacccacgac gcacaaccgc cgcttctggt
      601 cgcgggccgt catctgcgca tcacctgcga tagctcgctc acgccggtgc cgagcacttg
      661 gtcgacccgc acgggtgccc ccggccgcgc cgcgtcgcgc ggcaccgggc cgtcgacggc
      721 gacgcgcacc acgtcgctct gggtggcacc caccaggccg aggctttctt cgcagtgccg
      781 cgacacgcgc gacttggacg tcatggtggc gtagcgggcg gtgagcacgc cgatacgctc
      841 gcgcagcacg agctcctcac gcttgcgcgc cgccacgtct tccatgaggc tcgaggtgta
      901 cacctgggtc gacacgtatc cgagcaggag cacggcgaag gtcgcgtagg ccataaccat
      961 catgggcgca cccagtttgc cggccagcgc gtcgagcagg aactgtcccg cgaccaacat
     1021 gcgattgcgg cgcttcattg cacccggcct ccggatacgc gcgctccgtt cgagaccttg
     1081 gcggcggcgc gcaaggtcgc gctccgcgcg cgcgggttgg cggcgatctc gtcgtgcgac
     1141 gcgcgcacgc ccttgcgcgt caacacctcg aggcgcggaa cgcggccgca cacgcacacc
     1201 ggaacattgg gaggacacac gcaggtcgcg gcggcgtcgc gcagaaacgt cttcaccatg
     1261 cggtcttcga gcgagtggta gctcagaacc accaggcgcc cgcccggatt gaggacgtcg
     1321 agcacgccgt ccaggaacgc gcgtaaggcg tcgagctctc gattgatggc gatgcgcacg
     1381 gcctggaaga cgcgcgataa ttcggccggg gcaaccccgc gacccagcgc cgcgatcacg
     1441 gccgcgcgca gatccgacgt cgtggagaga gtgcccgccg cggccgcgct cgcaatcgcg
     1501 cgcgcgaccc ggcgggcctg gcggacctca cccaactcgc gaagcaatgt accaatctgg
     1561 tcgacatcga gacgcgccag catcagcgcg gcggtctcgc cttcgccgtc catgcgcatg
     1621 tcgagggggc cgtgggccga atgcgcgaag ccgcgcgcgg ggtcgtcgag ctgcgggctc
     1681 gagaagccga ggtcgagcag cacgccgtcg accttaccca cgcccgcaag cgcgtgctcg
     1741 aagtccgagt agagcccgcg caccaagtgc acgcgatcgg cgtagccgcg caagcgcacc
     1801 gcggcggcct ccagcgcggc cgggtcgcgg tcgacgccga tcaaacggat gtcggggcgc
     1861 gcggacagga tcgcctccgc gtgcccgccg aaaccgaccg ttccgtccat cacgacgcgc
     1921 gtgcgctcgt ggagcaggta gcgcacgacc tcgtcaacca tgaccggcag gtgctgcatc
     1981 gcgttatcct tgcgcgcttg cgcgccggac catccgaatg gaacgactac catcgtcatg
     2041 cgctgcgcct agaagaacgt gttgttgatg tgcttgaacg ccttcgccat ctcggcgtaa
     2101 cggcgttcga tgtgcttggg actccacact tcgacgcacg agctcatgtg aatgacgacg
     2161 acggtgctct cgagctggca gcggtcgcgc aggctcttgg gcaagatcag ccgcccttgc
     2221 gcgtcggcca ccacggtctc gcggtggagt tgtacaaagc gcacgaaatc gcgcttcaaa
     2281 tcgccggggg cctggctcaa gaatcggcgc tcgtactcgt cccactgggc ggtggtgcga
     2341 acctccagga atggcgggtc ctcgatattg ggcagaagat ggagacggtc gcccttcttg
     2401 agcttcagca gctcgcgcac ggggcgcgcg agttggagcc ggcccttggg gtcgatgccg
     2461 tggacgtgtt tctcgccgaa ggtgccctgc atcagcaaag cctaactcgt tggaaacgtt
     2521 actatttagg acagatttag ggaacttgca ccacttgaat gcgcattcta gggattttta
     2581 gggatcacgg tcaagggaaa atcctgcggc agggataatt atccacggcc cgcccaaaag
     2641 cggtcccgga agcggcgagg aggggtggga ttaggagggc ttggaggcca ctacgcacac
     2701 ggtcgcggcc aacccgcgca cgcccggcgt ccgttccgaa gtcttctcca aaacggtcca
     2761 cggcgcgtac cacgcgtcgg gcgtgctctt gagcatgaag agaaagtgcg acacctcggt
     2821 cacgcggaag ccggcgccct cgagcagcga tacgatctcg cggtcgggca gcggccggtg
     2881 gtaacgatac gggtgatagt catcgcgcgt ggaccccagg tccggataac acatcgacgg
     2941 cagcttcgcg cggatccacg ggtgacgcac cgcgacgcgc ttcgcgcgct cgaccaggct
     3001 cgcgtggttg ggtgtggaga gtacgatgcg gcccccgggg cgtaaaacgc gaaagcactc
     3061 ggccatcgcg cgcggccggt cctcggcgaa gatgtgctcg aaggtctcga ccagcgttac
     3121 gtgatcgaag cgcgcgtcgc cgaacggaag cgcgcgcgcg tccgcgccgg cgacgtcgaa
     3181 cggaatgcgg ttgtagtccg cgatgcgctt gcccaccacg aacgggtcgg gcacgatgtc
     3241 cacgccgacc gcgtggaaac cgagcgcgtt gagcgccatc acgttgaatc cccacgccga
     3301 gcccacgtcg agcacgcggc gccccgccgc gagatccagg tgccggtcca cttccatgaa
     3361 gcggcgcatc atcatgtgac ggcgcgcgcg tgtgcccaga taccagtggc gcaacaggtc
     3421 gggcatgtcc gcgcgcgcga gatcgggcgg gaccgcgatg tccggcagca cgggcgtcat
     3481 tgaaccaccg tcaagcgata caaaacataa tgaaacactc ccggcttgcg gatgcccagg
     3541 ttacgcactt cggtagcgta cacgcgctcg aagcggcgcc cgcccacgat ccccccgtcg
     3601 aagcgatcgg cttggtgatc gcgatcgacg aagtagtcca cgtgcgggta gccgggcacg
     3661 tcgaaataga gcccgcgcgc gacgatgtcc tcgtagtcgt gggcctcgcg cagcttcccc
     3721 gtttccggct cgaccagccc gcccaggtcg agcactcgcc gctgcgaata gaatgcgagg
     3781 tagccgatgt cggcggccgc caccaccgcg tcgggcggcg agttgtcgcg caggtacaac
     3841 gccagctcgg tcatgggacc ggtcaagtcg gcgctgaacg cgcgcgaggg cgggagcacg
     3901 acgcgcacgt acagcg
//
LOCUS       DASPGK010000400         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_214800, whole genome shotgun sequence.
ACCESSION   DASPGK010000400 DASPGK010000000
VERSION     DASPGK010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214800"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VEC56_11035"
     CDS             <1..595
                     /locus_tag="VEC56_11035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV14727.1"
                     /translation="VVYRRPYRSPPDQKNWAVARARNDWVLVLDADEALDESTRAEIA
                     NLDPGTHTGFWIRRRNDYLGRTIRGCGWQRDKVLRLYDRRRGHYPDAWVHEEVVLEGE
                     AGWLSGRLRHTPYRDVAHHLDKIDAYTTRGARQYVERGGRAALLNMLVHPPFRFLRMY
                     ALQWGVRDGHPGLVLCLLSSYSVFLKYAKAWERARRA"
     gene            571..1668
                     /locus_tag="VEC56_11040"
     CDS             571..1668
                     /locus_tag="VEC56_11040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14728.1"
                     /translation="MGARAQSMRGVCVFGGHHAGYPRSAVILAGLARLGVSVVSCVEN
                     PRKKVVRRYAGLVRKWRHLDRGFDVVFVPEFRHKDVPLAHLLTRRAGLPCVFDPLVSR
                     YDTRILDRADADARGPQAWHNKNLDRVSFALADLILADTRAHADLFAEQLAPPDTPIR
                     VLEVGCDDAVFHPAPPPPDAGVATVLFYGSYLPLHGVPVVVEAAERLRDDPRLRFELV
                     GDGQTRAEVERAVRERRLANIVMTPRVPAAELPKRIAGATVCLGIFGSTPKAARVVPN
                     KVYQCMGMARAVVTMDSRAVRETFQVGEEIVATPPGDGEALAFAIRGLIENREYRERV
                     ARAGAARVARDFTPVPIARRFAAYCEEACAR"
     gene            1665..>2722
                     /locus_tag="VEC56_11045"
     CDS             1665..>2722
                     /locus_tag="VEC56_11045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14729.1"
                     /translation="MTRDPRARETPDARVLRVLMVDSEESWRGGQAQVRLLMSGLVER
                     GVAVTLAAPSAGELYRRCEGLAIERRPLSLGWSGIVALRRAMASGQFDLVHSHAARAH
                     GMVAIARVGLSPRPYHVVSRRVDFAVARSPASAWKYRRGADAYIAISQGVRGVLVAGG
                     IPPARVRVVASGIDLAKFDGLKERAAVRAELGLSAGSFAVGNVAALAPHKAQDDLLGA
                     AARVLAQRDDVRFFIVGEGSLRARLEGLAGELGIDGRVFFTGFRTDALDLLRAFDVFV
                     MSSYLEGLGTSIMDAQALGVPVVATRTGGIPELVEDGVTGLLVPPRDPDALAAAILRF
                     LADAGLRGTCAQAARVRS"
BASE COUNT          363 a          919 c         1002 g          438 t
ORIGIN      
        1 ggtggtatac cggcggccgt accggtcgcc gcccgaccaa aaaaactggg cggtggcgcg
       61 cgcgcgcaac gactgggtgc tcgtgctcga cgccgacgag gccttggacg agtccacgcg
      121 agcggagatc gcgaatctgg atccgggaac ccacacgggc ttctggatcc gccggcgcaa
      181 cgactacctc ggccgtacca tccgcgggtg tggatggcag cgcgacaagg tgctgcgcct
      241 gtacgaccgc cggcgcggac actatccgga tgcgtgggtc cacgaggaag tcgtcctgga
      301 aggcgaggcc ggctggcttt cggggcgcct ccggcacacg ccctaccgcg acgtcgcgca
      361 ccatttggac aagatcgacg cgtatacgac gcgcggcgcg cgccaatacg tcgagcgcgg
      421 cgggcgcgcg gcccttctca acatgctcgt gcacccgccg tttcgattcc tgcgcatgta
      481 cgcgctgcag tggggcgttc gcgacggcca tcccggtctg gttctgtgcc tgctgtcctc
      541 gtacagcgtg tttctcaagt atgcgaaggc gtgggagcgc gcgcgcagag catgaggggc
      601 gtgtgcgtgt tcggcggcca tcacgcgggc tacccgcgca gcgccgtgat tctcgcggga
      661 ctcgcgcgcc tcggcgtttc cgtggtgagc tgtgtcgaga atccccgcaa gaaggtggtg
      721 cgtcgctacg cgggactggt gcgcaaatgg cgccacctgg accgtggatt cgacgtggtg
      781 ttcgtgcccg agtttcgtca caaggacgtg cccctggccc acctgctcac acggcgcgcc
      841 ggcctcccgt gcgtgttcga ccccttggtc tcgcgctacg acacccgcat cctcgatcgc
      901 gccgacgcgg acgcgcgcgg cccgcaggcg tggcacaaca agaacctgga tcgcgtctcc
      961 ttcgcgctcg cggacctgat cctggccgat acgcgcgcgc acgccgacct cttcgccgaa
     1021 cagctcgccc cgcccgacac gcccattcgc gtgctcgaag tcggttgcga cgacgccgtg
     1081 tttcaccccg cgcctcctcc ccccgacgcc ggcgtcgcga cggtactctt ctacggcagc
     1141 tacttgccgt tgcacggcgt cccggtcgtg gtcgaagccg cggagcgatt gcgcgacgat
     1201 ccgcgccttc gcttcgaact cgtgggcgac ggccaaaccc gggccgaggt ggaacgcgcg
     1261 gtacgcgagc gacgccttgc gaacatcgtg atgacgccgc gcgtgccggc cgccgagttg
     1321 ccgaagcgaa tcgcgggcgc cacggtgtgt ctcggcatct tcggctcgac gcccaaggcg
     1381 gcgcgtgtcg tgcccaacaa ggtataccag tgcatgggaa tggcgcgcgc cgtcgtcacc
     1441 atggactcgc gcgccgtgcg cgagacgttc caggtggggg aagaaatcgt ggccacgccg
     1501 cccggagacg gcgaagcgct ggccttcgcg atccgggggc tgatcgagaa ccgggagtac
     1561 cgcgagcgcg tcgcgcgtgc gggtgccgcg cgggtcgcgc gtgacttcac accggtgccg
     1621 atcgcgcggc gcttcgccgc ctactgcgag gaggcgtgcg ctcgatgacg cgcgacccac
     1681 gggcccgaga aacccccgac gcgcgcgtct tgcgcgtgct gatggtcgac tccgaggagt
     1741 cgtggcgcgg cggccaggcg caggtgcgct tgctgatgtc ggggctggtc gagcgcggcg
     1801 tggcggtgac cctcgcagcg ccgtcggcgg gcgagctcta ccgtcgctgc gaggggctcg
     1861 ccatcgagcg gcgtccgctc tcgctcggct ggtccgggat cgtcgcgctc cggcgcgcga
     1921 tggcgagcgg gcagttcgac ctcgtgcact cgcacgcggc gcgcgcgcac gggatggtgg
     1981 ccatcgcacg ggtcggcctc tcgccgcgcc cgtatcacgt cgtcagccgg cgggtcgact
     2041 tcgcggtggc gcgttctccg gccagcgcgt ggaagtaccg gcgcggcgcg gatgcgtaca
     2101 tcgcgatctc gcaaggggtg cggggcgtgc tggtcgcggg cgggatcccg cccgcgcgtg
     2161 tgcgcgtggt ggcgagtggc atcgacctcg cgaagttcga cggactgaag gaacgcgcgg
     2221 cggtgcgcgc ggagttgggc ctgagcgcgg gctccttcgc ggtcgggaac gtggcggcgc
     2281 tggcgccgca caaagcacag gacgatctcc tgggtgcggc ggcgcgggtg ctcgcgcagc
     2341 gggacgacgt ccgcttcttt atagtgggag aaggctccct gcgcgcgcgc ttggaaggac
     2401 tcgcggggga actgggcatt gacgggcgcg tgttcttcac ggggtttcgc accgacgcgc
     2461 tcgatctgct gcgcgccttc gacgtcttcg tcatgagctc gtacttggag gggctgggca
     2521 cgtcgatcat ggacgcgcag gccctgggcg ttccggtggt ggccacgcgc accgggggaa
     2581 tcccggaact ggtcgaggac ggcgtaaccg gtctgttggt accgccgcgc gatccggacg
     2641 cgttggccgc ggccatactc cggtttctcg cggacgcggg gctccggggc acttgcgccc
     2701 aggccgcgcg cgtgcgatcg ta
//
LOCUS       DASPGK010000401        10169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_683210, whole genome shotgun sequence.
ACCESSION   DASPGK010000401 DASPGK010000000
VERSION     DASPGK010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683210"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1084
                     /locus_tag="VEC56_11050"
     CDS             62..1084
                     /locus_tag="VEC56_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HXV14730.1"
                     /translation="MPPWFTFAGDGSARPAGEPACAVGGQTKRRDFASLADLGCAVTS
                     PWHNDVHVAIGGSMGGTFTAPEDPVFWRWHRFVNTIADNYLDDRANAPGIPPDLAFTN
                     PPRGYGLASLTSISVDFAEPVTGVVAADLEVNGSPATSVSGSGGGPYLFTGFFAPGAG
                     PVSVELGPGSIVDINDGMPMPSTAAWAYSINACVDADADGVYEDHCWPNPSFDNCPDV
                     FNPDQSDENNNEIGDACEGSTGIDDGDVPPARSKILSIAPNPFSASTRVAFDLASGHG
                     VRIEIFDPQGKRVALLVNRWLEAGNHRYDWNGRNGAGHAVPNGVYFLRLTAGTTRDVV
                     KIVVLR"
     gene            complement(1172..3205)
                     /locus_tag="VEC56_11055"
     CDS             complement(1172..3205)
                     /locus_tag="VEC56_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14731.1"
                     /translation="MNSINHNTIRLLAVCGLLALAACSEQAAAPQEPENAGYQPRTLT
                     RDDLAPEGVVVETADGYQVQGALHVKTDEGEVSFVNADLDVSFDENGRVRNITGTTEI
                     PSPHERIEFANPVQADIGFFSGKFLNDNRDFILLKEDTDYFVFNFDVNLEMRIATGET
                     GEDATKPISVRAPLGGQLLMIIDYNDPMYYVYGEQDLLGAAGMGWSLHSRIPFAPWHP
                     VEDMGTFDGKNTRVGTFPILKVLEVTGQMVDNNYTELHLSEEDPLTSDLRTGYQSGYN
                     GEMALDLFLKDIVGVEIPIAEGSGGVWSEVSVQEVFEGHAYVNGLTSELDWWPAFIPA
                     APVASLRTSAFIRSDLDFDVTVAGEFGWNLAAGGGNAWSGYFNFAPEALTLGGSVRDD
                     DTVFNIEGRVTKETTSVSTRPPQEVLDLVSSGVTGELDDRIAQAEQAWNDLQEATADY
                     EFELSLRGLRSALPNIVDTAKNALSTAIANELRNHEGEPYYDDLRDHLYSADNPYHTA
                     LDRLKAAALQIQDNDQTRAEIEAALRGVAARKIFSTTYVYRVLGVTVKTVNVSRRIMS
                     DTQANQIITAADNVRHIKESWDRKVRMQEIYDTIPDKEIFERVKDDIENGLIVIDDLG
                     EIGFVYGHDDQVFRAYALVGGDRHELGEVDLFSVVDLVAAVTSAIVDDLVSGD"
     gene            complement(3326..5668)
                     /locus_tag="VEC56_11060"
     CDS             complement(3326..5668)
                     /locus_tag="VEC56_11060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV14732.1"
                     /translation="MSTDERFARARDLFERAVEMTTAERAVFLDRECSGDVALRREVE
                     SLVAASENARDFLADSPASPWAGRRFAGYTILGAIAEGGMGVVLRARQEHPARDVAIK
                     LIRRDALAEKSQKRFQLEAEVLGRLDHPGIARIFSAATTDDGQPYFVMEYIEGVPVTH
                     YVRERNLPVRDRVALMIRIADAVHHAHANGVVHRDLKPANILVRSDGQPKVLDFGVAR
                     VTDSDVQLTTLHTEVGQVLGTLPYMSPEQVRGRHQDVDARSDIYSLGVVSYELLTGRL
                     PHDVHGKSLPEAARIISEDEPESLSARGGSFPADLQTIVGKCLEKERPRRYETARALG
                     EDLGRFLADEPVTARPPSASYQLRRFARRHRAVVVAVSSAFVILVAGLAVSIAGWSSA
                     SRERRRASVESERVGVLNQFLTDMLAAPDPYEAGADVRVVDVLDRASARIDSMLADQP
                     EVAVSAHRTLAQTYRSLDIGDKAELHFSRAIAITKSRVSGEASPELLRDYENLGNFYL
                     DQRRMSEADSIGAIVTSMMPQVPAESMEYLESCLFLAWLDEANGRVKEGADRLRPCLA
                     TMVARFGDEHARSVDARVALGNMCWQAGDLDEARTHLEKGLEQMTRLNGADHPATLAT
                     LNNIAFVYRESGDNDRALAAFEQSLRGRMIAYGAESWPTALGYGNLAHQQRIMGRGED
                     ALPNHREAIAIVRRVLGDDDARVAMLRSSYGQTLFATGRLDEAQEEMLAAYAGLEAAF
                     GADHPRCHALAKSLADLYAARGDDERVQYYRMRSGATAAP"
     gene            complement(5665..6246)
                     /locus_tag="VEC56_11065"
     CDS             complement(5665..6246)
                     /locus_tag="VEC56_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXV14733.1"
                     /translation="MKAMPRPDEEFQRLVANSRSGEERSIDRVTALVYDELRRVAASQ
                     LRLERRDHTLQPTALVHEAYARLAEQSNLAWEDRLHFIHIAAAQIRRVLVDHARARNR
                     HKRGGGLERVTLSDEIAQAPPAVDMLALNEALERLDAHSHQDRAIVELKYFGGLTEPE
                     IASVLGIGERTVRRRWTFARAWLFKELSEGGAS"
     gene            6375..8750
                     /locus_tag="VEC56_11070"
     CDS             6375..8750
                     /locus_tag="VEC56_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14734.1"
                     /translation="MRYAQWIATFAVTCLVLLAPVRAAAQWVENGVNMTGSGIGMYNT
                     GTGDHTVLASGAFVFVAAPGILGGDTDHVFVQKFDRYGAKLWGTNGIVPDNGGAVIGG
                     TDLVPDGFGGVILAWEADGFVYAQRLDANGVGLWDAGGVIVDTAGGPPCLVGDSAGGA
                     LVVFRSSVYEAVRAQRLDAAGVPQLGPNGVAVTAQFGQEALLDVVDGVTGGAAPLAIV
                     TYRVQGSNAIYAQKIDGAGNRLWGGFDLSLGPAMTFSIQDQRGQLSADETGGAWIAYE
                     VQVGSVRNIRVVRVSSAGSIAESFAIGTPNESSVLPKLAYVGGGKAHLAWWFGLTPAR
                     VAITELGSLFDAHYVALGAERPTDLVADGTGGSVLVWEHENGGFFDVYAARYHPASGT
                     PFPWGTGLGVPVCVASGSQFYAHMSRANTGVSGDYYLAWNDLRTASHIYVQRLDGGSG
                     VWGHPEPSIVAAADCEGDDEGGCLRLDWIKSQLDGDPFIHPQDPVTFYSIWRAVDVLP
                     PYPPDPICPQGMAAAGAGANRTTCEWEWVMNVNADGLPAYFANVPTTRDATPGDPATH
                     YFAVVAANETDGFYVSSVISGASHDEVAPDAPSGIDGDWIGSYVYVDWSPSVAPDVDL
                     YTVYHAERAGFGDPWSAWLPLGSSEDTTYTHTSADPALMHRYRATAVDESGNESGTVG
                     HVTVSANPTGIDSPGAAPSALRVLDASPNPFGDATTLRVGMPRNEAVEIDVFDARGRR
                     VHHETTKALGAGWHALRFEGRNTAGRLPSGVYFYRVRAGSETTTRKIVIVR"
     gene            complement(8759..>10169)
                     /locus_tag="VEC56_11075"
     CDS             complement(8759..>10169)
                     /locus_tag="VEC56_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14735.1"
                     /translation="VIMAGHFAGTANFGGANLPSAGVFDAFLARYTSAGTHVWSQRFG
                     GISNEFCYTVDINATGDIAIAGSFAQSADFGGGVFVSNGSSDTFVARYDAAGVHEWSR
                     SAGGALPDNAGALVFGADGDLVVTGYFQDVADFGGGDMGSAGLTDAFFARYSASAPEP
                     AITSIADIGNDQGRKVRIRFDRSGVDDEDAAVPVTRYVAFRRDDPPPAGVLAPPTPDA
                     RETLAAGWTEVGSVLAFAEESYGIDVATIGDSTIAQGQYYSVFYVRAATDVPSRFWDS
                     SPDSGYSVDNLAPGVPANLALAAGQLTWDESTAADFDYFTVYGANVDDFGAATVVDYT
                     VTPAMDVTSSPYAFYFVTATDFSGNEGNPAQVGTLTDVGETPRRYVLSISAYPNPFNP
                     ATTIRYTLPSRGRVVVTVYDARGMRIASLVDAERSAGAYTARWDGTDHAGSRVSSGVY
                     FARIAHNGATRTQKLVLLK"
BASE COUNT         1612 a         3408 c         3412 g         1737 t
ORIGIN      
        1 tgcggatggc gcgtacagca ttgcccacgc ggaccgggac tcgggggctc ccggtctacc
       61 catgccccct tggttcacgt tcgccggcga cgggagcgcg cgtccggcgg gcgaacccgc
      121 gtgcgccgtc ggaggacaga ctaaacgccg cgatttcgcg agtcttgccg accttggttg
      181 cgcggtcaca tcgccctggc ataacgatgt tcacgttgcg atcggtggct cgatgggcgg
      241 aaccttcacc gcgcccgaag acccggtctt ctggcggtgg caccggtttg tgaacaccat
      301 cgccgacaat tacctcgacg accgcgcgaa tgcgcccggg atcccaccgg acctcgcgtt
      361 cacgaatccg ccgcgcggtt acggcctggc ctctctcacg tcgatttcgg tcgactttgc
      421 agagccggtg acgggcgtag tggcggcgga cctcgaggtc aatggctctc ccgcgacgag
      481 cgtttccggc agcggcgggg gaccctattt gttcactggg ttcttcgcgc cgggtgcggg
      541 tccggtcagt gtcgaactcg ggcccggcag catcgtcgat atcaacgacg ggatgccaat
      601 gccgagcacc gccgcctggg cctactccat caacgcctgc gtggacgcgg acgccgacgg
      661 tgtctacgag gaccactgct ggcccaatcc ctcgttcgat aattgcccag acgtgtttaa
      721 tccagaccag tcggacgaaa acaacaacga aatcggcgac gcgtgcgagg gttccacggg
      781 cattgacgac ggcgacgtgc cgccggcgcg gtcgaagatt ctctccatcg caccaaaccc
      841 gttcagcgcg agcacgcggg tcgccttcga cctcgcgagc ggccacggag ttcggatcga
      901 gatttttgac ccacagggga agcgcgtcgc gctcctggtc aaccgctggc tggaggccgg
      961 gaaccaccgg tacgactgga acggaagaaa cggcgcgggc cacgcggtcc cgaacggagt
     1021 ctatttcctc cgactcaccg ccgggaccac acgtgacgtg gtgaagatcg tggtactgag
     1081 gtagcggcgt caagcaacgc gaaggcggcg gggttcacct tggaggtgat ccccgccgcc
     1141 tcgatcgcac gttgctatcg gcgccgtggc gtcagtcgcc cgacaccagg tcgtccacga
     1201 tggcgctcgt caccgctgcc acgaggtcga ccacactgaa cagatcgacc tcgcccagct
     1261 cgtgccggtc accgccgacc agcgcatagg ctcggaacac ctggtcgtcg tgtccgtaca
     1321 cgaacccgat ctcgcccagg tcgtcgatca caatgaggcc gttctcgatg tcgtccttga
     1381 cccgctcgaa gatttccttg tcgggaatcg tgtcgtagat ctcctgcata cggaccttgc
     1441 ggtcccacga ctctttgata tgccgcacgt tgtccgcggc cgtgatgatc tgattggcct
     1501 gcgtgtccga catgatgcga cggctcacgt tgacggtctt gacggtgacg cctaacactc
     1561 ggtagacgta cgtggtcgag aagatcttcc tcgccgcgac accgcgcagg gccgcttcga
     1621 tctcggcgcg ggtctggtcg ttgtcctgga tctgcagtgc cgccgccttc aggcgatcga
     1681 gtgccgtgtg atacggattg tccgccgagt acaggtgatc gcgcaggtcg tcgtagtagg
     1741 gctccccttc gtggttccgc aactcgttcg cgatcgccgt ggaaagcgcg ttcttggccg
     1801 tgtcgacgat gttgggtagc gccgagcgca acccccgcag gctcaactcg aactcgtagt
     1861 ccgcggtcgc ctcctgcagg tcattccacg cctgctcggc ttgcgcgatg cgatcgtcca
     1921 attcgccggt gacaccgctg ctcacgaggt cgaggacctc ttgcgggggc cgggtcgaca
     1981 cgctggtggt ctccttggtc acacggcctt cgatgttgaa cacggtgtcg tcgtcccgca
     2041 ccgatccgcc cagggtcagc gcttccggcg cgaaattgaa atagccgctc cacgcgttgc
     2101 ccccacccgc ggccaggttc cagccgaact cgcccgcgac ggtcacgtcg aagtcgaggt
     2161 cgctgcggat gaaggcgctc gtgcgcagcg acgcgaccgg ggcggccgga atgaacgccg
     2221 gccaccagtc gagttcggag gtcagtccgt tcacgtacgc gtgaccttcg aagacctcct
     2281 ggacgctcac ttcgctccac actccgccgc tgccttccgc gatgggaatc tccacgccga
     2341 cgatgtcctt caagaagaga tccagcgcca tctcgccgtt gtaaccgctc tggtatcccg
     2401 tgcgcaggtc ggaggtgagg gggtcttctt cggacaggtg caactcggtg tagttgttgt
     2461 ccaccatctg ccccgtgact tccagcacct tgaggatcgg aaacgtcccc acgcgcgtgt
     2521 tctttccgtc gaaggtgccc atgtcctcga ccggatgcca cggcgcgaag ggaatgcggc
     2581 tgtgcagcga ccatcccatg ccggcggcgc cgaggaggtc ctgctccccg tagacgtagt
     2641 acatggggtc gttgtagtcg atgatcatga gcagctgccc gcccaagggc gcccgcaccg
     2701 aaatgggctt ggtggcgtcc tcgcccgttt cgccggtggc gatgcgcatc tcgaggttga
     2761 cgtcgaagtt gaacacgaag tagtcggtgt cttccttgag aagaatgaag tcgcggttat
     2821 cgttcaggaa cttgcccgag aagaaaccga tatccgcctg cacgggattc gcgaattcga
     2881 tgcgctcgtg cggtgaggga atctcggtgg tgccggtgat gttgcgcacg cgcccgttct
     2941 cgtcgaacga cacgtccagg tcggcgttga cgaaggagac ttcgccctcg tcggtcttga
     3001 cgtgcaaggc gccttggacc tggtagccgt ccgcggtctc gacgacgact ccctcggggg
     3061 ccaggtcgtc gcgggtgagc gtccgcggct ggtagccggc gttctccggc tcctggggcg
     3121 ccgccgcctg ctcgctgcac gccgccaggg ccagcaaccc gcacaccgcg agcagccgta
     3181 tcgtattgtg attgatggag ttcatgacct cgtacctccg gggggccggg ccgaaccatt
     3241 cggccgtcta tagaggaacg caggaaatgg gcgggaaagc gatcacgccg gacgggttgg
     3301 gtgcggggtc cggtcccgac gacgactagg gggcggcggt cgctccggag cgcattcggt
     3361 agtactgcac ccgctcgtcg tcccccctcg ccgcatacaa gtccgcgaga ctcttcgcca
     3421 gggcgtgaca gcgcggatgg tcggcaccga acgcggcctc caaacccgcg tacgcggcca
     3481 gcatctcttc ctgcgcctcg tccaggcgcc cggtggcaaa gagtgtctgt ccgtaactgg
     3541 agcgcagcat ggcgactcgg gcgtcgtcgt cgccgagcac ccgccgcacg atggcgatgg
     3601 cctcgcgatg gtttggcagc gcgtcttctc cgcgtcccat gattcgctgc tggtgggcga
     3661 ggttgccgta gcccaaagcc gtcggccacg actcggcccc gtacgcgatc attctccccc
     3721 gcaggctctg ttcgaacgcg gccagtgcac gatcgttgtc gccgctctcg cggtagacga
     3781 acgcaatatt gttgagcgtc gccagcgtgg cggggtgatc ggctccgttg agtcgcgtca
     3841 tttgttcgag gcctttctcg aggtgggttc gcgcctcgtc cagatccccg gcctgccaac
     3901 acatgttgcc gagggccacg cgcgcgtcca ccgagcgcgc gtgctcgtct ccgaatcgcg
     3961 caaccatggt cgcgagacac ggccgcagcc ggtcggcgcc ctccttcacg cgcccattcg
     4021 cctcgtccag ccaggccagg aacaggcacg actccagata ttccatgctc tcggccggaa
     4081 cctgcggcat catggacgta acgatggccc cgatcgaatc cgcctccgac atccggcgct
     4141 ggtcgaggta gaagttgccc aggttctcgt agtcgcgtag aagctccggc gacgcctccc
     4201 ccgacacccg acttttcgtg atcgcgatcg ctctcgagaa gtgaagctcc gccttgtcac
     4261 cgatgtcgag cgagcgatag gtctgcgcga gcgtacggtg cgcgctgacc gcgacctcgg
     4321 gttggtccgc cagcatagaa tcgattcgcg ccgatgcgcg atcgagcacg tcgacgacgc
     4381 ggacgtccgc cccggcttcg tagggatcgg gtgccgccag catgtcggtg aggaactggt
     4441 tgagcacgcc cacccgttcg ctctccaccg atgcgcgcct gcgctcgcgc gacgcgctcg
     4501 accacccggc gatcgacacc gccaggccgg cgaccaggat cacgaatgcc gacgacaccg
     4561 cgaccaccac tgcgcgatgg cggcgcgcaa agcggcgcaa ctggtaggag gcactgggcg
     4621 ggcgcgccgt gaccggttcg tcggcgagaa aacgcccgag gtcttcgccc agcgcacgcg
     4681 cggtttcgta ccgtcgcggg cgttctttct cgagacactt tccgacgatg gtctggaggt
     4741 cggcgggaaa cgagccgccg cgcgcggaga gcgactccgg ctcgtcctcg gagatgatgc
     4801 gcgccgcctc ggggagcgac tttccgtgca cgtcgtgggg cagccgaccg gtgagcagtt
     4861 cgtaactcac cacccccaac gagtagatat cggagcgtgc gtcgacgtcc tggtgccgcc
     4921 cgcgcacctg ttcggggctc atgtacggaa gcgtgccgag cacctgcccc acctcggtgt
     4981 gcaaggtcgt cagctgcacg tccgagtccg tcacgcgcgc caccccgaag tcgagcacct
     5041 tgggctggcc gtcggaacga accaggatat tcgcgggctt gagatcgcgg tggacgacgc
     5101 cgttcgcgtg ggcgtggtgg acggcatccg cgatccggat catcagcgcc acgcgatcgc
     5161 gcacgggcag gtttctctcg cgcacgtagt gcgtcaccgg aacgccctcg atgtactcca
     5221 tcacgaagta gggctgcccg tcgtcggtgg tcgcggcgct gaagatgcgc gcgatgccgg
     5281 ggtggtcgag gcggccgagc acttcggcct ccagctggaa gcgcttctgc gacttctccg
     5341 cgagggcgtc ccggcggatg agtttgatgg cgacgtcgcg ggccgggtgc tcctggcgcg
     5401 cgcgcaggac gactcccatg ccgccctcgg cgatcgcgcc caggatcgtg taccccgcga
     5461 accgccgccc ggcccacggc gaggcgggcg agtcggccag aaagtcgcgc gcgttctcgc
     5521 tcgcagcgac gagggactcg acctcgcgcc gcagcgcgac atcgccactg cactcgcggt
     5581 cgagaaatac cgcgcgctcg gcggtcgtca tctcgaccgc acgctcaaag agatcgcgcg
     5641 cccgtgcaaa gcgctcgtcc gtgctcacga tgcccctcct tcgctcaact ctttgaacag
     5701 ccacgcgcgc gcgaacgtcc agcggcggcg tacggtgcgc tcgccgatcc cgagcaccga
     5761 cgcgatctcg ggctcggtca gcccgccgaa gtacttgagc tcgacgatgg cgcggtcctg
     5821 gtgcgagtgc gcgtccaacc gttccaacgc ttcgttgagg gccagcatgt cgacggcggg
     5881 cggcgcctgg gcgatctcgt cggacagggt gacccgctcc agcccgccgc cgcgcttgtg
     5941 ccggttgcgc gcgcgggcgt gatcgaccag gacgcggcga atctgggcgg cggcgatgtg
     6001 gatgaagtgg aggcggtcct cccaggcgag attgctctgc tcggcgaggc gcgcgtacgc
     6061 ctcgtggacg agggcggtcg gctgcagggt gtggtcgcgg cgctcgagac gcaactggct
     6121 ggccgccacg cggcgaagtt cgtcgtagac cagagcggtc acacggtcga tcgagcgctc
     6181 ctccccggac cgggagttcg ccacgagcct ctggaactcc tcgtcgggac ggggcatggc
     6241 tttcattata gccgatcgcg cccggccgaa ttctcgagtg gccggattcg acctggattg
     6301 acgcaaaccc attcaggcga gcgagaatcg ccgccgtcgc accgcgcccc ctttcgtgcc
     6361 caggaggaac tcccgtgaga tacgcccaat ggattgccac gtttgcagtt acctgtctcg
     6421 tgctcctcgc ccccgtgcgc gcggcggccc aatgggtcga gaacggggtg aacatgaccg
     6481 ggtccggcat cggcatgtac aacaccggaa ccggagatca caccgttctt gcgagcggag
     6541 ccttcgtatt cgtggcggca cccggaatcc tgggcggcga cacggaccac gtgttcgtcc
     6601 aaaagttcga tcggtacgga gcgaagcttt ggggaaccaa cggcatcgtt cccgataacg
     6661 ggggcgccgt catcggcggc accgacctcg tcccagacgg ttttggcggg gtgatcctgg
     6721 cctgggaagc cgacggcttc gtctacgcgc agcgcctcga cgcgaacggt gtcggcttgt
     6781 gggacgcggg tggggtcatc gtcgacaccg ccggagggcc gccgtgcctc gtcggcgaca
     6841 gcgcgggggg cgcgctggtg gtcttccgca gctcggtcta cgaagccgtt cgagcacagc
     6901 ggctcgatgc ggccggcgtc ccgcagctcg ggcccaacgg cgttgcggtc acggcccagt
     6961 tcggccagga ggcccttctc gacgtcgtcg acggggtgac gggcggtgcg gctccgctgg
     7021 cgatcgtcac ctaccgcgtg cagggatcca acgccatcta cgcgcaaaaa atcgacggcg
     7081 ccggcaaccg gctgtgggga ggtttcgacc tgtcgctcgg gccggcgatg acgttttcga
     7141 ttcaagacca gaggggccag ctctctgccg acgagaccgg cggcgcgtgg atcgcgtacg
     7201 aggttcaggt cggcagtgtg cggaatattc gcgtcgttcg cgtatccagc gcgggctcga
     7261 tcgcggagtc gttcgcgatc ggaacgccga acgaatcgtc ggtcctgccc aaactggcgt
     7321 acgtcggcgg cggcaaagcc cacctggcgt ggtggttcgg tctcacaccg gcgcgcgtcg
     7381 cgatcacgga attgggcagt ctgttcgacg cccattacgt ggcactcggg gcggagcgcc
     7441 cgacggatct ggtcgcggac ggcaccggcg gatcggtgct ggtgtgggag cacgagaacg
     7501 gcgggttctt cgacgtctac gcggcgcgat accaccccgc gtcgggcact ccttttccct
     7561 ggggtacggg tctcggcgtg cccgtttgcg tcgccagcgg aagccaattc tatgcgcaca
     7621 tgtcccgcgc caatacgggc gtgtcgggag actactatct cgcgtggaac gatctgcgaa
     7681 ccgcgagcca catctacgtc cagcgcctcg acgggggaag cggcgtgtgg ggccaccccg
     7741 aaccgtcgat cgtcgcggcc gcggattgcg aaggcgacga cgagggtggt tgcttgcgac
     7801 tcgattggat caagagccag ctcgacggag acccgttcat tcatccccag gatccggtta
     7861 cgttctactc gatctggcgc gcggtggacg tgctcccgcc gtacccgccg gatcccatct
     7921 gcccgcaggg catggccgcc gccggggcgg gcgcgaatcg caccacgtgc gagtgggagt
     7981 gggtcatgaa cgtcaacgcc gacgggctcc cggcgtactt cgcgaacgtc ccgaccacgc
     8041 gcgacgccac cccgggcgac cccgccacgc actacttcgc ggtcgtcgcc gcgaacgaga
     8101 ccgacggctt ctacgtttcg tcggtcatca gcggcgcgtc acacgacgaa gtggcgccgg
     8161 acgccccctc cggcatcgac ggagactgga tcgggagtta cgtgtacgtc gactggtcgc
     8221 cgtcggtcgc tcccgacgtg gatctttaca ccgtctatca cgcggagcgg gccggcttcg
     8281 gcgatccgtg gagcgcgtgg ctgccgctgg gttcgagcga ggacaccacg tacacccaca
     8341 cgagcgccga ccccgcgctg atgcaccggt atcgcgcgac cgccgtcgac gagagcggca
     8401 acgagagcgg gaccgtcggg cacgtgaccg tttcggcgaa tcccaccggt atcgactccc
     8461 ccggtgcggc gccgtcggcg ttgcgcgtgc tggatgcgtc acccaatccc ttcggcgacg
     8521 cgaccacgct gcgagtgggg atgccccgga atgaagccgt ggagatcgac gtgttcgacg
     8581 cgcgcgggcg tcgcgtgcac cacgagacca cgaaggcgct gggggccggc tggcacgcgc
     8641 tgcgcttcga gggccgcaac accgcgggcc gcttgcccag cggcgtgtac ttctaccgcg
     8701 tgcgcgccgg cagcgaaacc acgacgcgga agatcgtgat cgttcgttga ggctcgcttt
     8761 acttcagcag cacgagcttc tgcgtccgcg tcgctccgtt gtgggcgatg cgcgcaaagt
     8821 aaacgcccga gctcacgcgc gatccggcat ggtcggtgcc gtcccagcgc gcggtgtagg
     8881 ccccggccga gcgctccgcg tccaccagtg acgcgatccg catcccgcgg gcgtcgtaca
     8941 ccgtgaccac gacccgtccc cgcgacggga gcgtgtaccg aatcgtggtc gcggggttga
     9001 agggattcgg gtacgcggag atcgagagca cgtagcggcg cggggtctcg cccacgtcgg
     9061 tgagtgtgcc gacctgcgcc gggttgccct cgttgcccga gaagtcggta gcggtgacga
     9121 agtagaacgc gtacggcgag ctggtgacgt ccatcgcggg cgtcacggtg tagtccacga
     9181 ccgtcgccgc gccgaagtca tccacgttcg cgccgtagac ggtgaaatag tcgaagtcgg
     9241 ccgccgtcga ttcgtcccac gtgagctggc ccgccgcgag agcgaggttg gcggggactc
     9301 ccggcgccag attgtcgacc gagtacccgc tatcgggcga ggagtcccag aagcggctcg
     9361 gcacgtcggt cgcggcgcgc acgtagaaca ccgagtagta ctgcccttgc gcaatggtcg
     9421 agtcaccgat ggtggcgacg tcgatgccat acgactcttc ggcgaacgcg agcaccgacc
     9481 cgacttcggt ccacccggcc gccaatgtct cacgcgcgtc gggcgtgggt ggcgccaaca
     9541 cgccggccgg tggcgggtcg tcgcggcgga acgcgacgta ccgcgtgact ggaaccgccg
     9601 cgtcctcgtc gtcgacaccg gatcggtcga aacgaatcct caccttgcgc ccctgatcgt
     9661 tgccgatgtc cgcgatcgac gtgatggccg gctcgggcgc gcttgcggag tagcgggcga
     9721 agaacgcgtc ggtgagcccg gcgctcccca tgtcgccgcc cccgaaatcc gccacgtcct
     9781 ggaagtaccc ggtcaccacg aggtcgccgt ccgcgccgaa aacgagggcg ccggcgttgt
     9841 cgggtagcgc gccgcccgcg ctccggctcc actcgtgcac gccggccgcg tcgtaccggg
     9901 cgacgaacgt gtcgctggac ccgttgctaa cgaacacacc gcccccaaag tcggccgact
     9961 gggcgaagct gcccgcgatc gcgatgtcgc cggtcgcgtt gatgtcgacg gtgtagcaga
    10021 actcgttcga tatccccccg aagcgctggc tccacacgtg cgtgcccgcc gaggtgtatc
    10081 gggcgagaaa cgcgtcgaac acacccgcgc tcgggagatt ggcgccgccg aaattggcgg
    10141 tcccggcgaa gtgtcccgcc atgatcacg
//
LOCUS       DASPGK010000402         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_333474, whole genome shotgun sequence.
ACCESSION   DASPGK010000402 DASPGK010000000
VERSION     DASPGK010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333474"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEC56_11080"
     CDS             <1..279
                     /locus_tag="VEC56_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXV14736.1"
                     /translation="AALQADALVFLTDVPGVRDAEGVVMPSLDIRRLPSMVQEAVVSG
                     GMLPKLEACNRALRRGVPRVHILPAAWADDVRRLSNGGIERGTEVVAR"
     gene            276..1499
                     /locus_tag="VEC56_11085"
     CDS             276..1499
                     /locus_tag="VEC56_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HXV14737.1"
                     /translation="MKTHLDMIRQQESQLLVPTYDRVPVLFERGEGVHLFDSEGRRYV
                     DFLSGIGVNALGHGHPAIQAAIAAQAGRLIHVSNLFFHEHQARLAAKLAALSGLDRAF
                     FCNSGTEAWECALKLARAYGRHTNSNGSKPKSRLVVMTDSFHGRTLGSLATTGNKKYR
                     APFAPMLPGVTFVKFNDVADLESKFDHSVCAVGLETIQGEGGVNPVSREFLARARALT
                     REAGALLILDEIQCGLGRTGAMFAYQKYGVVPDVVTVAKPLAAGLPLGAVLATHAVAA
                     AFRPGMHGTTFGGGPLACAAAIAFLDEIAKPEIREHVATIGDYFRERLLALKSKHRVV
                     VDVRGVGLMLALELESADQAKSIVNTALARGYVINRTHDTVLRFLPPYIIAREHVDGL
                     VAVLDELLSGGSEHE"
     gene            1492..2697
                     /locus_tag="VEC56_11090"
     CDS             1492..2697
                     /locus_tag="VEC56_11090"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXV14738.1"
                     /translation="MNDKVVLAYSGGLDTSIIIPWLKENYDCDVVAYVGDVGQGEDLD
                     AVADKAYATGASKVVVEDLRDEFLNDYVFPAIRAGAVYEHKYLLGTALARPVIAQHQA
                     ELALREGASALAHGCTGKGNDQVRFELAFRAVAPELKIIAPWREWALVSREDCLAYAE
                     SRGIPVEQSRAKIHSRDRNLLHVSHEGGELEDPDHAPLESTWTWTCSPQDAPDRTEAV
                     EIAFEEGIPVSVNGVRLGPVVLMELLNEIGARNAVGRVDMVENRFVGIKSRGCYETPG
                     GTLLLAAYRELEALCLDRDLAHYKQQLSLKYAELVYYGQCFTPLRESIDAFVESTRRA
                     ITGAVTLSLYKGNVAVTGRRSVNSMYRTDVASFTMSDAYDQKDAAGFIRIVGLQTRGR
                     VAQQMEAVE"
BASE COUNT          450 a          910 c          948 g          467 t
ORIGIN      
        1 gcggcgctgc aggcggacgc gctggtgttc ctgaccgacg tccccggggt gcgcgacgcg
       61 gagggcgtcg tcatgccgtc gttggacatt cgtcggctcc cgtcgatggt gcaggaagcg
      121 gtcgtgagcg gcgggatgtt gcccaagttg gaagcgtgta accgcgcctt acggcgcggt
      181 gttccgcgcg tgcatattct tccggccgcg tgggccgacg acgtgcgccg gctctccaac
      241 ggcgggatcg agcgcggaac cgaggtcgtc gcgcgatgaa gacccatctg gacatgatcc
      301 ggcagcaaga gtctcagctc ctggtcccga cgtacgatcg cgtgccggtg ctcttcgagc
      361 gcggcgaagg cgtgcacctc ttcgacagtg agggacgtcg ctatgtcgat ttcctctcgg
      421 gtatcggcgt caacgcgctc ggacacggcc acccggccat ccaggccgcg atcgccgcgc
      481 aggcgggacg gctgatccac gtgtcgaacc tgttctttca cgagcaccag gctcgcctgg
      541 ccgccaagct ggccgcgctc tcgggcctcg accgcgcctt cttctgcaac agcgggacgg
      601 aggcgtggga gtgcgcgttg aagctagcgc gcgcctacgg acgccacacg aactcgaacg
      661 gatcgaagcc gaagtcgcgc ctggtggtca tgacggactc gttccacggg cgcacactcg
      721 gttcgctcgc gactaccggc aacaagaagt accgggcacc gttcgcgccc atgctgccgg
      781 gcgtgacgtt cgtgaagttc aacgacgtcg cggatctcga gtcgaagttt gaccactcgg
      841 tgtgcgcggt cgggctggag accatccagg gcgaaggcgg ggtcaacccg gtgtcccgcg
      901 agttcctcgc ccgggcgcgc gccctgacgc gcgaagcggg cgcgctcctc atactggacg
      961 agatccagtg cgggctcggt cgcacgggcg ccatgttcgc ctatcagaaa tacggcgtcg
     1021 tacccgacgt cgtcacggtg gccaagccgc tcgcggccgg gctgccgctc ggggccgtgc
     1081 tggccacgca cgcggtcgcg gccgcgtttc gacccgggat gcacgggacc accttcggcg
     1141 gcgggccgct cgcgtgcgcc gcggccatcg cgttcctgga cgagatcgcg aagccggaga
     1201 tacgcgagca tgtcgctacc atcggcgact actttcgcga acggttgctc gcgctgaaat
     1261 cgaagcaccg cgtggtcgtc gatgtgcgcg gagtcggtct catgttggcc ctggagctcg
     1321 agtccgccga ccaggcgaaa tcgatcgtca ataccgcgct cgcgcgcggc tacgtcatca
     1381 atcgtacgca cgacaccgtg ctgcgctttc ttccgccgta catcattgcg cgggaacatg
     1441 tggacgggct cgtcgccgtg ctcgacgaat tgctatcggg aggtagcgaa catgaatgac
     1501 aaggtggtac tcgcctattc gggtggtctc gacacgtcga tcatcatccc gtggctcaag
     1561 gagaactacg actgcgacgt cgtcgcctac gtgggcgacg tcggccaggg cgaagacctg
     1621 gacgcggtcg ccgacaaggc gtacgcgacc ggcgccagca aggtcgtcgt cgaggatttg
     1681 cgcgacgagt ttctcaacga ttacgtgttc cccgctatcc gcgcgggcgc ggtctacgag
     1741 cacaagtacc tgctcggcac cgccctcgcg cggcccgtca tcgcgcagca ccaagccgag
     1801 ctcgcgctgc gcgaaggcgc ttccgccctg gcgcacggct gcaccggcaa gggcaacgac
     1861 caggtgcgct tcgagctggc gtttcgcgcg gtcgcgcccg aattgaagat catcgcgccc
     1921 tggcgcgaat gggcgctggt ctcgcgcgag gactgtctcg cgtacgcgga gtcgcgcggc
     1981 atcccggtcg agcagagccg cgccaagatt cacagccgcg atcgcaacct cctgcacgtg
     2041 agtcacgagg ggggcgagct ggaggatccc gaccacgccc cgctcgagtc gacctggacc
     2101 tggacgtgtt cgccgcagga tgcgcccgac cgcaccgagg ccgtcgagat cgccttcgag
     2161 gaagggattc cggtctcggt caacggcgtg cgtctgggac ccgtcgtgct gatggagcta
     2221 ttgaacgaga tcggcgcgcg caacgcggtc ggccgcgtcg acatggtgga gaaccgcttc
     2281 gtcggcatca agtcgcgcgg ctgctacgag acgccgggcg gaacgctgct gctagccgcg
     2341 tatcgcgagc tggaagcgct ctgcctcgac cgcgacctcg cgcactacaa gcagcagctg
     2401 tcgctcaagt acgcggagct ggtgtactac ggccagtgtt tcacgccgct gcgcgaatcg
     2461 atcgacgcct tcgtcgagtc gacgcgccgc gccatcaccg gtgccgtgac gctgtcgctc
     2521 tacaagggca acgtcgccgt caccgggcgc cgctcggtca attcgatgta ccggaccgac
     2581 gtcgcgagct ttacgatgag cgacgcctac gaccagaagg acgcggccgg tttcattcgt
     2641 atcgtcggat tgcagacgcg cggccgggtc gcgcagcaga tggaggccgt cgaatgaaga
     2701 tgtggtcggg aagattcgaa gcgccggtgg aggcgtcgtt cgaccgctgg caacggtcgt
     2761 ttccctatga ccgcc
//
LOCUS       DASPGK010000403         4416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_800758, whole genome shotgun sequence.
ACCESSION   DASPGK010000403 DASPGK010000000
VERSION     DASPGK010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800758"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VEC56_11095"
     CDS             <1..1676
                     /locus_tag="VEC56_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXV14739.1"
                     /translation="TITTFEEGQIVRGTVTDIRENEVIVDVGFKSEGTIPAQEFRTID
                     NLSVGMQFDVYLEKLENQDGLVVLSKEKADFFKVWGQIKDSHDMNQIVQGRVKRRIKG
                     GLVVDLLGVDAFLPGSQIALRQVPNLDDLLGQTLDFKIIKINKRRRNIVVSRRVVLEE
                     KREELKRGLMSELEAGQIREGQVKNVTDFGAFVDLGGVDGLLHLTDMTWGRVGHPSDV
                     VQIGQKIKVKVLDFDREKEKISLGLKQLSPHPWEGVNERFPVTTRVKGRVVSITDYGA
                     FIELEKGVEGLIHISEMSWTKHVKHPSKILSVGQEIEAVVLKVDKDAQKISLGLKQTE
                     PDPWTTIERRYPHGTRLKGIVRNLTNFGAFVEIEEGIDGLLHISDMSWAKRVRHPSEV
                     VKKGDLVDVVVLEISQDKKRISLGMKQLTDNPWERLAVEFGPDTEVTGKITRILDRGA
                     VVELRHDVEGFVPVGQMGFDDIVHPGQYFQPNEEVPLKVIKVDPKNKRIVLSISAYLK
                     ARGDDALREFVAAHPKRDFVIPATPSGEGDDIDDADLGAGDVVETSSDD"
     gene            1846..2166
                     /locus_tag="VEC56_11100"
     CDS             1846..2166
                     /locus_tag="VEC56_11100"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXV14740.1"
                     /translation="MWFIRSFLILVGVIAFLWLGMENADQTVDFTFFTKEYPGLNLNF
                     LMLLVFVSGMVFSFLISVINEIALRRQLAQTRRELTRMDREIAALRSLPLEDTEGLKS
                     GSQL"
     gene            2183..3343
                     /locus_tag="VEC56_11105"
     CDS             2183..3343
                     /locus_tag="VEC56_11105"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14741.1"
                     /translation="MGDAPVIPFLAGVAVALLVVGARWTVRRDQQSRRVERATDPYIE
                     GLKLLVDGKKPEAMVRLHQAIMAGGAPADAYVRIGRMLRENGDASKALQVHKSLTVKA
                     DLTREEKVAVYANVAEDYAALGHPERALETVEVATRRTGLRDAELSAIAARACHALGR
                     TEEAYEHMKELKKAGGTTDREIALYLVTVAEKESDKGRARDAKKTLARALRHDPECAP
                     ALVAMARLEEQADDVGDAIRLWRQAAQLSPDLAPVALRSLERALYQRGTFNEIESVYR
                     DVLETRPNDEHAALGLASFYRKQGRMDDAMHLLEEYRGTNPATVAGTVLLSSLYAARG
                     DTDELESFLDRSDRMLARDERYRCGSCGYEASEMRWHCPRCNRFDTFARTPR"
     gene            3315..4376
                     /locus_tag="VEC56_11110"
     CDS             3315..4376
                     /locus_tag="VEC56_11110"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV14742.1"
                     /translation="MTRSHALRASAAIAALVFAWAPATTARDDAGDLMRRAQRDAEAG
                     RFVAADSGYARAAEIAEGDERAEALFRRAGVVRSGTIAESLYRTILDEYGEGEWGRPA
                     ALELAKIQFAMGRYESARGVLAGADLCAWSDEACVFDGMAAIMLRRFDEAAAPLSSVK
                     RGRHKTWATISLAEAEEGMGHEASACERYAGLARARVHPTAWLRHTECLDAAGDAEGG
                     KRELEALADAFPYSPEALRASAKLSPPPPQAAATPSGDAAASGVEPAQPPLGGTGFTI
                     QFGSFADRGNAIKLAAKIKKTYPGVRIDSELVNYREVFRVRYGQYATRGEAQTAGETM
                     TRELDERYTVMPVTRAADE"
BASE COUNT          799 a         1464 c         1501 g          652 t
ORIGIN      
        1 cgaccatcac gaccttcgag gaaggtcaga tcgtgcgcgg cacggtcacg gacatccgcg
       61 agaacgaagt catcgtcgac gtcggattca agagcgaagg cacgatcccg gcgcaagagt
      121 ttcgcaccat cgacaacctc tcggtcggca tgcagttcga cgtctacctg gagaagctcg
      181 agaaccagga cggcttggtc gtcctctcca aggagaaggc ggactttttc aaggtctggg
      241 gccagatcaa ggactcgcac gacatgaacc agatcgtgca gggccgcgtg aagcgtcgca
      301 tcaagggcgg cttggtggtc gacctcttgg gcgtcgacgc gttcctgccc ggcagccaga
      361 tcgcgctgcg gcaggtcccc aacctcgacg acctcctcgg ccagacgctc gatttcaaga
      421 tcatcaagat caacaagcgc cgccgcaaca tcgtggtctc gcgccgcgtc gtgctcgaag
      481 aaaagcgcga ggaattgaag cgcggcctga tgagcgagct cgaagcgggc cagattcgcg
      541 aaggccaggt caagaacgtc accgacttcg gcgccttcgt cgacttgggc ggcgtcgacg
      601 gtctgttgca cttgaccgac atgacgtggg gccgcgtggg tcacccgtcg gacgtggtgc
      661 aaatcggcca gaagatcaag gtcaaggtgc tcgacttcga ccgcgagaag gagaagatct
      721 cgctgggctt gaagcagctc tcgccgcacc cgtgggaggg cgtcaacgag cgcttcccgg
      781 tcacgacgcg ggtcaagggc cgggtcgtca gcatcaccga ctacggcgcc ttcatcgagc
      841 tcgagaaggg tgtggagggc ttgattcaca tctccgagat gtcgtggacc aagcacgtca
      901 agcacccctc gaagattctc tcggtgggcc aggagatcga ggcggtcgtt ctcaaagtcg
      961 acaaggacgc gcagaagatc tcgttgggcc tgaagcagac cgagcccgat ccgtggacca
     1021 cgatcgagcg ccggtatccg cacggcacgc gcctcaaggg gatcgtgcgc aacctgacca
     1081 acttcggcgc cttcgtcgag atcgaagagg gcatcgacgg cctgttgcac atctccgaca
     1141 tgagctgggc caagcgcgtg cggcacccga gcgaagtggt caagaagggc gacctcgtcg
     1201 acgtggtggt gctggagatc agccaggaca agaagcgcat cagcctggga atgaagcagc
     1261 tcacggataa cccgtgggag cgcctggcgg tcgagttcgg accggacacc gaagtgaccg
     1321 gcaagatcac ccgcatcctc gatcggggtg cggtggtcga gcttcgtcac gacgtggaag
     1381 gcttcgtgcc ggtcggacag atgggcttcg acgacatcgt ccaccccggg cagtacttcc
     1441 agcccaacga ggaagtgccg ctcaaggtta tcaaggtgga tccgaagaac aagcgcatcg
     1501 tgctcagcat cagcgcctac ttgaaggcgc gcggcgacga cgcgcttcgc gagttcgtgg
     1561 cggcgcatcc caagcgcgat ttcgtcatcc cggcgacacc ctcgggcgag ggcgacgaca
     1621 tcgacgacgc cgacctcggg gccggcgacg tggtcgagac gtcttcggac gactagccgc
     1681 agcggcgcgt tccattgctc cacgagggcc ggtcagccaa tgaccggccc tcctcgtttc
     1741 ggggcctggg aaagccgccc ttgacgcccc ccgttccacc ggccatgatg ggttgcccct
     1801 tggagttatc gcggggggcg tcgacccggg aaggaggctt cggccgtgtg gtttatccgc
     1861 tcattcttga tcctggtggg cgtcatcgcc ttcctttggc tgggcatgga gaacgcggac
     1921 cagaccgtgg acttcacgtt cttcaccaag gagtacccgg ggctcaatct gaatttcctg
     1981 atgctgctcg tcttcgtgtc cgggatggtg ttctcgttcc tgatctcggt catcaacgag
     2041 atcgcgctgc ggcgccagct cgcgcaaacc cgccgcgagc tcacacgcat ggaccgcgag
     2101 atcgcggcac tgcgcagcct gccgctggaa gacacggaag gactcaagag cggctcgcaa
     2161 ctctaaccgg ccgaccaacg ccatgggtga cgctcccgta atcccgtttc tcgccggcgt
     2221 cgccgtcgcc ctcctggtcg tgggcgcgcg ctggaccgtg cgtcgcgacc agcagtcgcg
     2281 gcgcgtcgag cgcgccaccg atccctatat cgaaggcttg aagcttttgg tcgacggcaa
     2341 gaagccggag gcgatggtgc gcctgcacca ggccatcatg gccgggggcg cccccgcgga
     2401 cgcgtacgtc cgcatcggcc gcatgctgcg cgagaacggc gacgcgtcca aggcgctgca
     2461 ggtccacaag agcctcacgg tgaaggcgga cctgacgcgc gaggagaagg tcgcggtgta
     2521 cgccaacgtg gccgaggact acgccgcgct gggacacccc gagcgcgcgc tcgagacggt
     2581 ggaagtggct acccggcgca cgggactgcg cgacgccgag ctgtccgcga tcgcggcccg
     2641 cgcctgccac gcgctggggc ggaccgaaga agcgtacgaa cacatgaagg agctcaagaa
     2701 ggcaggcggc accaccgacc gcgagatcgc gctctatttg gtgacggtcg cggagaagga
     2761 gtccgacaag ggacgcgcgc gcgacgcgaa gaagaccctc gcgcgggccc tccgccacga
     2821 ccccgagtgc gcgcccgcgc tggtcgcgat ggcgcgcttg gaagaacagg ccgacgacgt
     2881 aggcgacgcg attcgcctgt ggcggcaggc ggcacagctc tcgcccgact tggcgccggt
     2941 ggcgttgcgc agcctcgagc gcgcgctcta ccagcgcggc accttcaacg agatcgagtc
     3001 ggtgtaccgt gacgtcctgg agacgcgccc caacgacgag cacgcggccc tggggctcgc
     3061 gtcgttctac cgcaaacaag gccgcatgga cgacgccatg cacctgttgg aagagtaccg
     3121 cgggaccaac cccgccaccg tcgccgggac cgtgctcttg tcgtcgctct acgcggcgcg
     3181 cggggacacc gacgagctcg agtccttcct cgatcggagc gaccgcatgc tggcgcgcga
     3241 cgagcgctac cggtgcggct cgtgcggcta cgaggccagc gagatgcgct ggcactgccc
     3301 gaggtgtaac cgctttgaca cgttcgcacg cactccgcgc tagcgctgcc atcgccgcgc
     3361 tcgtgttcgc atgggcgccg gcgaccacgg cacgcgacga cgcgggcgac ctcatgcgcc
     3421 gcgcgcaacg cgacgcggaa gcggggcgct tcgtcgcggc cgattccggc tacgcgcgcg
     3481 cggccgagat cgccgagggc gacgagcgcg ccgaagccct gttccggcgc gcgggcgtgg
     3541 tgcgatccgg aaccatcgcg gaatcgcttt accgcaccat cctggacgag tacggcgagg
     3601 gcgagtgggg acgcccggcg gcgctcgagc tggccaagat tcaattcgcc atggggcgct
     3661 acgaaagcgc gcggggcgtg ctcgcgggcg ccgacctgtg cgcgtggtca gacgaggcgt
     3721 gcgtgttcga cgggatggcc gcgatcatgc tgcgccgctt cgacgaggca gcggcaccgc
     3781 tgtcgagcgt caagcgcggt cgccacaaga cctgggccac catttcgctc gcggaagccg
     3841 aggagggcat gggccacgag gcctcggcct gcgagcgcta cgccgggctc gcccgggcgc
     3901 gcgtccaccc gaccgcgtgg ctgcgccata ccgagtgcct cgacgccgcg ggcgacgccg
     3961 agggtggcaa gcgcgagctc gaagcactcg ccgacgcgtt cccgtactcg ccggaagcgc
     4021 tgcgcgcgtc ggccaagttg tctccgccgc cgccgcaagc ggccgcaacc ccgtccggcg
     4081 acgcggccgc cagcggcgtc gaacccgccc agcccccgtt gggcggaacc ggcttcacga
     4141 tccagttcgg ctcgttcgcg gaccgcggca acgcgatcaa gctggccgcc aagatcaaga
     4201 agacctaccc gggcgttcgc atcgacagcg agctggtcaa ctaccgcgag gtgttccggg
     4261 ttcgctacgg ccagtacgcg acgcgcggag aggcgcaaac cgcgggcgag acgatgaccc
     4321 gcgagctcga cgaacgctac accgtcatgc ccgtgacacg agccgccgat gagtgacgcc
     4381 accccgctca tgtcgcagta ccgggacatc aagaac
//
LOCUS       DASPGK010000404         4170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1034002, whole genome shotgun sequence.
ACCESSION   DASPGK010000404 DASPGK010000000
VERSION     DASPGK010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034002"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VEC56_11115"
     CDS             <1..431
                     /locus_tag="VEC56_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HXV14743.1"
                     /translation="MAACEKKEQAQSGRVENTPDKVAITSPVGFVWKWEGTQTPVESI
                     VPTDPTRYTLEFLPDTSVVAQVDCNRGRGKYQLDGQSITIGPLAVARMACPEGSLDVQ
                     FLQHLEAARHIFFRGDTLYMDLFADSGTMKFSQLPEPPRP"
     gene            428..1474
                     /locus_tag="VEC56_11120"
     CDS             428..1474
                     /locus_tag="VEC56_11120"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXV14744.1"
                     /translation="MKTLVVLLLAGGDSAERDVSLSSSRGIAKALREAGHRVLVADPA
                     QPNVTPTEYDEAIFDGAAIGIEPPQVGGDVAHARSAFVRMLAARDDWDFDVVFNGLHG
                     GAGENGTMQAVLDYLQIPYTGSGAAASALAMDKQRAKIVAAAAGVPVPQGLHVERSAL
                     AAGTLEQQVRETLGLPVIVKPNAQGSSVGLTLVHSFAELDEAARRAFAVDDSILIEPY
                     IEGREITQAMVEGAPDLPLLEIKPKSGLYDYAHKYQSGGSEYLVPAPVDEKIAREVLV
                     SSKRAFAALGLAIYTRFDYRLDPQGRHYLLEANTLPGMTATSLVPKAAAAVGISYTEL
                     CDRIVRESLARFRS"
     gene            complement(1479..2810)
                     /locus_tag="VEC56_11125"
     CDS             complement(1479..2810)
                     /locus_tag="VEC56_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXV14745.1"
                     /translation="MLPTSAQPARVPVKALGIWSCTALVIGNIVGSGVFLLPASLAPY
                     GIYALVAWVLTALGAVALALVFGRLARAIPKSGGPYAYTRAAYGDFAGFWVAWGYWIC
                     LWTGVAAIAVAFGSYLKVFIPALDGNPILCGAASIAAVWVLAFVNGLGLRSASLVQIV
                     TTVVKLLPLVAIAIVGVAWIDLPRIAAPIALEVGPFGAVSSAATLALWAFLGLESATI
                     PAEAVDRPERTIPRATVLGTLVCAVVYIASTVVLMGALPRETLASSQAPFADAARVMW
                     GDWAYYAVGLGAVVSCFGALNGWILVAGQFPTAVARDGLFPSRFATVSARGTPTFSIM
                     AAVVLVTVMLTLNYSGSNSLVSIFNFAILLSTLATLIPYVFCSLAPLLLSRPQVGIMP
                     AARARDRVVMTIAFVYSVWAIYGAGAQTVLLGFLLMLAGIPVYVWQRRALK"
     gene            2863..>4170
                     /gene="cysS"
                     /locus_tag="VEC56_11130"
     CDS             2863..>4170
                     /gene="cysS"
                     /locus_tag="VEC56_11130"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXV14746.1"
                     /translation="MPLRVYDTLKQKKTDFVPIHPGKVGMYVCGMTVQDKPHMGHMLA
                     FVSGDMIRRYLVYLGYDVVYVQNFTDIDDKIITRANEEGVDYRVVVERNIEAYHRIAR
                     EVNILPATHYPYATHHIPEIIAMVAALIEKGHAYAVGGDVYFRVRTKPDYGKLSKRDI
                     DDLISGSRVEVGEKKEDPLDFALWKSSKPGEPAWESPWGQGRPGWHIECSAMAVKILG
                     ETIDFHGGGEDLIFPHHENELAQSECATGKEFVRSWMHNGLLNLRGQKMSKSTGHFFG
                     MEDVLKEFRGEVVRFYLLSTHFRSQMEYSRERLENAKSALERLENACRSLDERIARLG
                     KGPSVSTPAGRTLVEAAREAKVRFLEAMNDDFNSAGAIGYLFELIKTYNVLLDEDEAS
                     VSQSREGLEAVWSALSEFDRILGLFKSGLPRAQEDVPAQIQQMV"
BASE COUNT          806 a         1377 c         1374 g          613 t
ORIGIN      
        1 ccatggccgc gtgcgagaag aaagagcaag cgcaatcggg cagggtcgag aacacgcccg
       61 acaaagtcgc gatcacctcg ccggtgggat tcgtgtggaa atgggaaggc acgcaaaccc
      121 cggtcgagag catcgtccca accgacccca cacggtacac gctcgagttc ctgcccgaca
      181 cctccgtggt ggcgcaagtc gactgcaacc gcggccgggg caagtaccag ctcgacgggc
      241 agtcgatcac gatcggcccc ctggcggtcg cgcgcatggc gtgcccagaa ggatcgctcg
      301 acgtccagtt cctgcagcac ctggaagccg cccgccacat cttcttccgc ggcgacacgc
      361 tctacatgga cctgttcgcg gattcgggga cgatgaagtt ctcgcagctg ccggagccgc
      421 cgcgaccatg aaaacgctgg tcgtgctcct gctcgccggc ggcgactccg ccgagcgcga
      481 cgtgtcgctg tcgagctcgc gcgggatcgc gaaggcgctg cgcgaagcgg gccaccgcgt
      541 gctggtcgcc gaccccgcgc aacccaatgt gacgcccacc gagtacgacg aggccatctt
      601 cgacggcgca gcgatcggca tcgagccgcc ccaggtgggg ggtgatgtgg cccacgcgcg
      661 ttccgccttc gtgcgcatgc tcgccgcgcg cgacgactgg gacttcgatg tcgtgttcaa
      721 cggactccac ggcggtgcgg gcgagaacgg caccatgcag gcggtcctcg actatctgca
      781 gatcccgtac acgggatccg gcgcggcggc cagcgcactc gccatggaca agcagcgcgc
      841 caagatcgtc gcggccgcag ccggcgttcc cgtgccgcag gggcttcacg tcgagcgctc
      901 cgcgctcgcg gccggaaccc tcgaacagca ggtacgcgag acgctgggcc tgccggtgat
      961 cgtcaagccc aacgcgcagg gatcgagcgt ggggctgaca ctcgtgcatt cgttcgccga
     1021 actcgacgaa gccgctcgcc gcgccttcgc ggtcgatgat tcgattctga tcgagcccta
     1081 catcgagggg cgcgagatca cgcaggcgat ggtcgaaggc gcgcccgatc ttcccttgct
     1141 cgagatcaag ccgaaatcgg ggttgtacga ctacgcgcac aaataccagt cgggcggctc
     1201 cgagtacttg gtccccgcgc cggtcgacga gaagatcgcg cgcgaggtac tggtgtcgtc
     1261 gaagcgcgca ttcgccgcac tcggactcgc gatctatacg cgcttcgact accggctgga
     1321 tccccaaggc cgccactatc tgctcgaagc gaacacgttg cccgggatga cggcgaccag
     1381 cttggtgccg aaagccgcgg ccgcggtcgg tatttcctac acggagttgt gcgatcgcat
     1441 cgtgcgggag tcgctggcga gatttcggtc gtagtcgttt acttcagcgc tcgacgctgc
     1501 cacacgtaga cgggaattcc cgccagcatc agcaggaacc ccaggagcac cgtctgtgcg
     1561 ccggcaccgt agatcgccca caccgagtag acgaacgcga tcgtcatcac gacgcggtcg
     1621 cgcgcacgcg ccgcgggcat gatccccacc tgcggacgcg agaggagaag gggcgcgagc
     1681 gagcagaaca cgtacggaat cagcgtcgcg agcgtggaaa gtagaatcgc gaaattgaag
     1741 atcgagacca ggctgttcga tcccgagtag ttgagcgtca gcatgaccgt caccagcacg
     1801 accgcggcca tgatcgagaa ggtgggcgta ccgcgcgccg ataccgtggc gaagcgcgac
     1861 gggaagagcc cgtcgcgcgc gaccgcggtg gggaactgcc ccgccacgag gatccagccg
     1921 ttgagcgcgc cgaagcacga caccaccgct cccaacccca ccgcgtagta cgcccagtcg
     1981 ccccacatca cgcgcgccgc gtcggcgaag ggcgcctgcg acgacgcgag cgtttcgcgc
     2041 ggcagcgccc ccatcaacac gaccgtgctc gcgatgtaga ccaccgcgca caccagtgtt
     2101 cccaatacgg tcgcgcgcgg gatggttcgc tcggggcggt cgacggcttc ggcgggaatg
     2161 gtcgcggact ccagtcccaa gaacgcccac agcgcgaggg tggctgccga tgacaccgcg
     2221 ccgaacggac ccacctcgag cgcgatgggc gccgcgatgc gcgggaggtc gatccacgcc
     2281 accccgacga tcgcgatcgc gaccagcggc agcagcttca cgacggtcgt gacgatctgc
     2341 accaggctcg cgctgcgaag cccgagcccg ttgacgaatg cgagcaccca cacggccgcg
     2401 atcgacgccg cgccgcacag gatcgggttc ccgtccagcg cggggatgaa caccttgaga
     2461 taactcccga acgcgaccgc aattgcggcg acacccgtcc acaggcagat ccaataaccc
     2521 cacgcgaccc agaagcccgc gaagtcgccg taggccgcgc gcgtgtaggc gtagggcccg
     2581 cccgacttcg ggatcgcgcg cgcgaggcgt ccgaacacca aggcgagcgc gaccgcaccg
     2641 agtgccgtca acacccaggc gacgagcgcg tagattccgt acggggcgag ggaagcaggc
     2701 aggaggaaga cgccggagcc gacgatgttg ccgatgacca gcgcggtgca cgaccagatg
     2761 ccgagcgcct tcacgggaac gcgggccggt tgcgccgagg tgggaagcat cgcgcgagcc
     2821 agtagaccac acgcgagcag ccctgctata gtgctttctc ccatgccgct gcgcgtctac
     2881 gacaccctca agcagaagaa gaccgacttc gtccccatcc acccgggcaa ggtcgggatg
     2941 tacgtgtgcg gcatgaccgt ccaggacaag ccgcacatgg ggcacatgct ggccttcgtg
     3001 tcgggcgaca tgattcggcg atacctcgtg taccttggct acgacgtggt ctatgtccag
     3061 aacttcaccg acatcgacga caagatcatc acgcgcgcca acgaagaggg tgtggactac
     3121 cgcgtggtgg tggagcggaa catcgaggcg taccaccgca tcgcgcgcga ggtgaatatc
     3181 ctccccgcga ctcactaccc gtacgccacc caccacatcc cggagatcat cgcgatggtg
     3241 gcggctctga tcgagaaggg gcacgcctac gcggtcgggg gcgacgttta cttcagggtc
     3301 cgcaccaagc ccgattacgg caagctctcc aaacgcgaca tcgacgactt gatttccgga
     3361 tcacgagtgg aagtcggtga gaagaaagaa gatccgctcg acttcgcgct ttggaagtcg
     3421 tccaagccgg gcgaaccggc gtgggagagc ccgtggggac agggacgccc cgggtggcac
     3481 atcgagtgct cggcgatggc ggtgaagatc ctgggcgaga ccatcgactt ccacggcggc
     3541 ggcgaggact tgatcttccc gcaccacgaa aacgagctcg cgcagagcga gtgcgccacc
     3601 ggcaaggagt tcgtccgctc gtggatgcac aacggcctct tgaacttacg cggccagaag
     3661 atgtcgaagt cgaccggcca cttcttcggc atggaagatg tgctcaagga gttccggggc
     3721 gaagtggttc gcttctatct gctatcgacc cacttccggt cccagatgga gtactcgcgc
     3781 gagcggttgg agaacgcgaa gagcgccctc gagcgcctcg agaacgcctg ccgttctctg
     3841 gatgagagga tcgcccggtt ggggaagggc ccttcggtca gcactccggc gggccgcacg
     3901 ctggtcgagg ccgcccggga ggcgaaggtc cgcttcctcg aggccatgaa cgacgacttc
     3961 aacagcgccg gtgcgatcgg ttatctgttt gaattgataa agacttacaa cgttctcctg
     4021 gacgaggacg aggcctcggt cagccagtcg cgggagggcc tggaggcggt ctggtcggcg
     4081 ctctcggagt tcgaccgcat cctcgggttg ttcaagagcg ggctgccgcg ggcccaagag
     4141 gatgtccccg cacagatcca gcagatggtt
//
LOCUS       DASPGK010000405         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1621276, whole genome shotgun sequence.
ACCESSION   DASPGK010000405 DASPGK010000000
VERSION     DASPGK010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621276"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..332)
                     /locus_tag="VEC56_11135"
     CDS             complement(27..332)
                     /locus_tag="VEC56_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HXV14747.1"
                     /translation="MTTTLREKIIEALKTCYDPEIPIDIWELGLIYDIDIADSNDVTI
                     TMTLTTPHCPAAETLPLDVENKVTAVEGVASVKVDITWEPAWSPQMMSEAARLELNL"
     gene            complement(335..790)
                     /locus_tag="VEC56_11140"
     CDS             complement(335..790)
                     /locus_tag="VEC56_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXV14748.1"
                     /translation="MSDMRELYQEMILDHHKHPRNFGRLTGANRHAEGFNPLCGDRVT
                     VYALVEGDEIRDLSFEGSGCAICMASASVMTDELAGQTIDDAERLYVRFHDLVTGESA
                     KEPDLDALGKLAVFMGVREFPVRVKCATLPWHALRAALKDGEKAPVSTE"
     gene            complement(787..2028)
                     /locus_tag="VEC56_11145"
     CDS             complement(787..2028)
                     /locus_tag="VEC56_11145"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXV14749.1"
                     /translation="MTRTTKATIDVTAVRRDFPILEREVHGKPLAYLDNAATSQKPRA
                     VIDAISSYYATVNANVHRGVHTLSVRATELYEGAREKTRAFVNAADAAEIVFVRGTTE
                     AINLVASSFGQARVRSGDEVVISTMEHHSNIVPWQLLCQRAGAKLRVIPMDRTGDLRL
                     DEYRTLLNERTRIVAIPHVSNSLGTVNPMAEMIAIAHEHDIPVLVDGAQAVPHLRVDV
                     RALGADFYAFSGHKMFGPTGAGVLYARRALLETLPPYQGGGDMIASVTFEKTTYNVLP
                     HRFEAGTPDIAGAVGLGAAIDYLDALGFDAIEAYENELVAYAVDVLSSTPGVTLIGTP
                     KHRVGVVSFTIDGVHPHDAGMILDHEGIAIRAGHHCTQPVMDFYGVPATNRASFAFYN
                     TRAEVDRLVAAIAKVKEVFRS"
     gene            complement(2052..>2910)
                     /gene="sufD"
                     /locus_tag="VEC56_11150"
     CDS             complement(2052..>2910)
                     /gene="sufD"
                     /locus_tag="VEC56_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXV14750.1"
                     /translation="IAEGVTVDRPIHIVHMTSASEAPVMTHPRVVVVVGDNSEAVVVE
                     SFVGLGTGAYFTNAVAQFTVGANAGLRHYKIQRETPDAFHVAFQHAALARSARFSTLG
                     VTMGGALVRNDVHTILNGEGIDARVDGLYLADGRRHVDNHTFIRHAQPHCHSFELYKG
                     ILDDKARGVFNGRIYVDPVAQKTDAKQENNCMLLSDEARINSNPQLEIFADDVKCTHG
                     AAIGKTDDTAIFYLRSRGIPFQEARDLMVYAFASEVLERIELEPLKRRLEDDLFEWLE
                     QSRRARARA"
BASE COUNT          443 a          934 c          991 g          542 t
ORIGIN      
        1 tgggtagagc atctcgttcg tcctcctcac aggttcaatt cgagccgggc cgcctcggac
       61 atcatctgcg gcgaccacgc gggttcccag gtaatgtcga ctttcaccga cgcgacccct
      121 tcgaccgcgg tcaccttgtt ctccacgtcg agcggcagcg tttctgcggc cgggcagtgg
      181 ggtgtggtca gcgtcatggt gatcgtcacg tcgttcgagt cggcgatgtc gatgtcgtag
      241 atcagcccca gctcccagat gtcgatcggg atctcggggt cgtagcacgt cttcagggct
      301 tcgatgatct tctcgcgcaa tgtcgtcgtc atggtcattc ggtggaaacg ggcgccttct
      361 cgccgtcctt gagggccgcg cgcagcgcgt gccacggcag ggtcgcgcac ttgacgcgca
      421 ccgggaactc gcgcacgccc atgaacaccg ccagctttcc cagcgcgtcg aggtcgggct
      481 ccttggccga ctcgcccgtc accaggtcgt ggaacctcac gtacaagcgc tcggcgtcgt
      541 cgatggtctg gcccgcgagc tcgtcggtca tgaccgaggc gctcgccatg cagatcgcgc
      601 acccgctgcc ttcgaaggat aggtcgcgaa tctcgtcgcc ctcgaccagc gcgtacacgg
      661 tgacgcgatc cccgcacagg gggttgaaac cttccgcgtg ccggtttgca cccgtgagcc
      721 gcccgaagtt gcgcgggtgt ttgtggtgat ccaggatcat ctcctggtag agctcgcgca
      781 tgtcgctcac gaacggaaca cctctttgac cttcgcgatg gccgccacca ggcggtccac
      841 ttccgcgcgc gtattgtaga acgcgaacga cgcgcgattg gtggccggca ccccgtagaa
      901 gtccatcacc ggctgcgtgc agtggtggcc ggcgcggatc gcgatgccct cgtggtcgag
      961 gatcatgccg gcgtcgtgcg ggtgcacgcc atcgatggtg aacgacacca cgccgacgcg
     1021 atgcttgggc gttccgatca aggtgacgcc gggcgtcgag gaaagcacat ccaccgcgta
     1081 ggcgacgagt tcgttctcgt aggcctcgat ggcgtcgaag cccagcgcgt ccaggtagtc
     1141 gatagccgcg cccaaaccga ccgccccggc gatatcgggc gtaccggctt cgaagcggtg
     1201 cggcagcacg ttataggtcg tcttctcgaa cgtcaccgac gcgatcatgt cgccgccgcc
     1261 ctgatagggg ggcagcgtct ctagcagcgc gcggcgggcg tacagcacgc cggcccccgt
     1321 cggcccgaac atcttgtgtc cggagaaggc gtagaagtcg gctcccagcg cgcgcacgtc
     1381 gacgcgaaga tgcggcaccg cctgggcgcc gtcgaccagc acggggatat cgtgctcgtg
     1441 cgcgatggcg atcatctcgg ccatcgggtt gaccgtgccg agcgagttcg acacgtgtgg
     1501 gattgcgaca atgcgcgtgc gctcgttgag cagggtgcgg tactcgtcca ggcgcaggtc
     1561 gccggtgcga tccattggga tcacgcgcag cttcgcgccc gcgcgctgac acaggagctg
     1621 ccacggcacg atgttcgagt ggtgctccat ggtcgagatg acgacttcgt cgccggagcg
     1681 cacgcgtgcc tgtccgaacg agctcgccac caggttgatc gcctccgtcg ttccgcgcac
     1741 gaacacgatc tcggccgcgt ccgcggcgtt cacgaaggcg cgcgtcttct cgcgggcgcc
     1801 ttcgtacagc tcggtggcgc gcacgctcag cgtgtgcacg ccccggtgca cgttggcgtt
     1861 gacggtggcg tagtagctcg agatggcgtc gatcacggcc cgcggcttct gcgaggtggc
     1921 cgcgttgtcg aggtaggcga gcggcttgcc gtgcacctct cgctcgagga tcgggaagtc
     1981 gcgacgcacg gccgtgacgt cgatcgtcgc cttggtggtt cgcgtcatgg tctggggctc
     2041 cgtatccacg gtcacgcccg tgcgcgcgcg cgccgagatt gctcgagcca ctcgaacagg
     2101 tcgtcctcga ggcgccgctt gagcggttcc aactcgatcc gctccagcac ctcgctcgcg
     2161 aatgcgtaca ccatcaagtc gcgcgcctcc tggaagggaa tcccgcgcga acggaggtag
     2221 aaaatcgcgg tgtcgtcggt cttgccgatg gcggcgccgt gcgtgcactt gacgtcgtcg
     2281 gcgaagatct cgagttgcgg gttcgagttg atgcgggcct cgtccgagag cagcatgcag
     2341 ttgttctcct gcttggcgtc ggtcttttgc gcgaccgggt cgacgtagat gcgaccgttg
     2401 aagacgcccc gcgccttgtc gtcgaggatg cccttgtaga gctcgaagct gtggcaatgc
     2461 ggttgggcat gccggataaa ggtgtggttg tcgacgtgcc ggcggccgtc ggcgaggtac
     2521 aggccgtcga cgcgcgcgtc gatgccttct ccattcaaga tggtgtgcac gtcgttacga
     2581 accagggcgc cgcccatggt gacgccgagg gtcgagaagc gcgcgctgcg cgcgagagcc
     2641 gcgtgctgga aggcgacgtg gaaggcgtcc ggcgtctcgc gctggatttt gtagtgccga
     2701 aggccggcgt tggcgccgac cgtgaattgt gcgaccgcgt tggtgaagta ggcgccggtg
     2761 cccaggccca cgaagctctc caccacgacg gcttcactgt tgtcgcccac gacgacgacg
     2821 acgcgcgggt gcgtcatgac cggcgcctcc gaagcgctcg tcatgtgcac gatgtggatg
     2881 gggcgatcca cggtcactcc ctcggcgata
//
LOCUS       DASPGK010000406         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1385078, whole genome shotgun sequence.
ACCESSION   DASPGK010000406 DASPGK010000000
VERSION     DASPGK010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385078"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..2875
                     /gene="sppA"
                     /locus_tag="VEC56_11155"
     CDS             290..2875
                     /gene="sppA"
                     /locus_tag="VEC56_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXV14751.1"
                     /translation="MSKRIVFISAVAATVVAGLSPDHARAQGYGAIPSYYQSLDFNLT
                     SPSAFTEAVGGFANPSIYPMMPGGEFEYYWSSFDDTFHDPKRWGIFTGFENIGFGANY
                     AKLRLPTGEVSITDYRIGIGGGTKRSTFGLGYGWSGGDEGTFGREDLIQAGLTIRPSS
                     LLSLSGAFNFSADSGKNQQLVDVALRPLGNDRLTIFGDMEFSSDDLSSATPWSAGAML
                     EVPAGLKIIGRWFDGDGAFGDGFSVGLAYTLGPHYQQGVMRASGVPRFDDDNHHTLTN
                     WGVRMGYPERSDLMKPLAEGRGYYQMSLKGAVPYTTFRYFDTRTPLEDILQSIDDARE
                     DYRVAGIAINLSGAKLSRGTAWEIRERLGELRATGKHVVVFVDELGMSTYYLASVADR
                     IVMDPEGLALLPGYAMGRTYLANMVEKIGLGVEEWRFLKYKSAMESLVRHEMSEGDRE
                     QRQALVDQFYATFREDVAQGRSVDAKTVDRWINDVTMFTAQQAVDEKLVDELGRWEEV
                     KEAAKALEGKKQPFIDRGDLANEWYPSKAWGERPQIAVVYAIGACAMDTGIQARKLEK
                     TLRRLRDDRDTKAIVLRVDSPGGSPIASDVVANQLRRCMEKKPVVISQGDVAASGGYW
                     LSMCSNQIVAQPTTVTGSIGVISGWVWDKGVGEKLGMEGDFVEAGEHADLFFSLRPPF
                     LPVPIPHRAITDEERERALAGMKSMYSGFVGAVAKNRKMTNERVEELAQGRVWTGVEA
                     KNNGLVDRIGGLHDAIQVARELAEIGPRDEVKVVEYGTRGLFRMPFESPSLSSPWAAI
                     ASVATFGAFQSLAAREMLGDRGEQADESAPDDYDLIYLQQIIRNNGRGLCMLPPEYLP
                     RDADR"
     gene            complement(3723..>4233)
                     /locus_tag="VEC56_11160"
     CDS             complement(3723..>4233)
                     /locus_tag="VEC56_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14752.1"
                     /translation="SDRDRLILTMFGAFLHQSMNTTQEVYGACRALYCSNQVYRVADL
                     PFFVFAEPATGRSVSEVSGILSDHVDRAVAMLDDDALVATVKGSIVSFVTASMLKPDV
                     PDYPLAHHQVIGQEALNVGMKHLLREGRSAEELTAEVNAITADEVRTTVRKYVTRDKL
                     VQITFGPRT"
BASE COUNT          751 a         1329 c         1404 g          749 t
ORIGIN      
        1 gatcttagcc acgtcgaagg gcgggtcctc ccagttgcgc tcgcggagct tcttcaaccc
       61 ctggaacatc ccgcaatcga ccaggacgtt cttcttgccg atggacagga cgtacttcga
      121 cccggtgacg gtctgcgccg ccccgtagaa cgagagaaac acccgcggcc cccttccctg
      181 gaggtgtaac tgccgccgtg attatccgta tgggtgagta caccgtcaac gctattgcga
      241 ccgtctgcct ccggcactag aatcgccctt taaggagggg ccccacgtca tgtcgaaacg
      301 tatcgtattc atatccgccg tcgcggccac ggttgtggcc ggtttgtcgc cggaccacgc
      361 gcgcgcccaa gggtacggcg ccatcccgtc gtactatcaa tcgctcgact tcaatctcac
      421 cagcccttcg gcgttcacgg aggcggtggg cggatttgcg aacccatcga tctacccgat
      481 gatgccgggc ggagaattcg agtactactg gtcgagcttc gacgacacgt ttcacgatcc
      541 caagcggtgg gggatcttca ccggcttcga gaacatcggt ttcggcgcca actacgccaa
      601 gctgcgcctg ccgaccggcg aagtgagcat caccgactac cgcatcggca tcgggggagg
      661 caccaagcgc tccaccttcg gcctgggcta cggatggtcg ggcggcgacg aggggacgtt
      721 cggtcgcgaa gacctgatcc aagccggcct caccattcgg cccagctcgc tgctctcctt
      781 gagcggcgcg ttcaacttca gcgccgactc ggggaagaac cagcagctgg tcgacgtggc
      841 cctgcgcccg ctgggcaacg accgcctgac gatcttcggc gacatggagt tctcttcgga
      901 tgatctctcg tcggccacgc cctggagcgc gggcgcaatg ctcgaagttc ccgccggctt
      961 gaagatcatc ggccgctggt tcgacgggga cggcgcgttc ggagacgggt tcagcgtcgg
     1021 actcgcgtac acgctgggcc cgcactatca gcagggtgtg atgcgcgcct cgggcgtgcc
     1081 gcgcttcgac gacgacaacc atcacacgct caccaactgg ggcgtgcgca tgggttaccc
     1141 cgaacgttcc gacctcatga agccgctggc cgagggtcgc ggctactacc agatgagcct
     1201 caagggagcg gtgccgtaca ccacgtttcg ctacttcgac acgcgcacgc cgctggaaga
     1261 tattctgcag tccatcgacg acgcgcgcga ggactaccgc gtagcgggca tcgccatcaa
     1321 tctctccggt gccaagctct cgcgcggtac ggcgtgggag attcgcgagc gcttgggcga
     1381 gctgcgcgcg accggcaagc acgtcgtcgt cttcgtggac gaactcggta tgtcgacgta
     1441 ctacctcgcg agtgtcgcgg accgtatcgt catggacccc gaaggtctcg cgctcctccc
     1501 cggctacgcg atggggcgga cctacctggc caatatggtg gagaagatcg ggctgggcgt
     1561 ggaggagtgg cgcttcttga aatacaagag tgcgatggag tcgctcgtgc gccacgagat
     1621 gagcgaaggc gaccgcgagc agcgccaggc attggtcgac caattttatg cgacgtttcg
     1681 cgaagatgtg gcgcaggggc gtagcgtcga cgcgaagacg gtcgaccgct ggatcaacga
     1741 cgtgaccatg ttcacggcgc agcaggccgt cgacgagaag ctggtcgacg agctgggccg
     1801 ctgggaagag gtcaaggagg cggccaaggc gctcgagggc aagaagcagc cgttcatcga
     1861 tcgcggggac ctcgccaacg agtggtatcc ctcgaaggcg tggggcgagc gcccgcagat
     1921 cgcggtggtg tacgcgatcg gcgcgtgcgc gatggacact ggaatccagg cccgcaaact
     1981 cgagaagacg ctgcgccgtc tacgcgacga ccgcgacacc aaggccatcg tgctgcgggt
     2041 cgattcgccc ggcgggagcc ccatcgcatc cgacgtggtc gcgaaccagc tgcgccgctg
     2101 catggagaag aagcccgtcg tgatctccca gggcgacgtg gcggcgtcgg gcggctactg
     2161 gctctcgatg tgctcgaacc agatcgtcgc gcagccgacc actgtcaccg ggtcgatcgg
     2221 cgtcatctcg ggctgggtgt gggacaaggg agtaggcgag aagctcggca tggagggcga
     2281 tttcgtcgaa gcgggcgagc acgcggacct cttcttctcg ctgcgaccgc cttttctccc
     2341 cgtgccgatc ccccaccgag cgatcaccga cgaagagcgc gagcgggcgc tcgccggaat
     2401 gaagtcgatg tattccggct tcgtgggcgc ggtggccaag aatcgcaaga tgacgaacga
     2461 gcgcgtggaa gagctcgcgc aggggcgcgt gtggaccggc gtcgaggcca agaacaacgg
     2521 cctcgtcgac cgcatcggcg ggctgcacga cgcgatccag gtggcccgag agttggcgga
     2581 gatcgggccg cgagacgaag tcaaggtggt cgagtacggc acgcgcggct tgttccgcat
     2641 gccgttcgag tcgccatcgc tgtcctcgcc gtgggcggcg atcgcttcgg tggcgacgtt
     2701 cggggcgttt cagagcctcg ccgcgcgcga gatgttgggc gaccgcggcg agcaggcgga
     2761 cgaatcggcc cccgacgatt acgacttgat ctacctgcag caaatcattc gcaacaacgg
     2821 acgcgggttg tgcatgctgc cgccggagta cctgccgcgc gacgcagacc ggtaaaagag
     2881 cgtccgttgc agcccccgcg cttcgggact atacttgcga tcgaccgcgc aagcggtcgg
     2941 agtcatgcaa gggcgggggc ccgcacggtg taagaccgag cggttcctcc ggactagaga
     3001 cgcgcaccat gggcaaccgt ggtgcgcgtc gttttgttcg gtgcatgaat tgtcgcagtg
     3061 cgcggcgcct gcgagacgcg cacgcgcaaa agaaagaagg agccgtcgga gggcagacga
     3121 ctccttcttt tcccgctatg gcgatccagt tgctgttcaa tgcgcgccga cccattcgac
     3181 gtcggggtgc ccgcacagcc tcatggactt gaattcgtca agatgggccg gcgcgcgctc
     3241 tcgttggcaa agttgatccc tcccccaaga ggaacccacg gtttcgattc gccctactgc
     3301 ggccccgcgc gcggttgcct ccacgaccca ccggctccca cgttcggttc cgacgattcg
     3361 ggcgtctcga gatcacccag gcggaggtta gaccgcgcct cctttcgttc cctgtgtccg
     3421 cgccccggcg cgaatcgaga cgtttttccg ggtgtcgggt tagcgacgcg tccgattcac
     3481 tcgcgccgaa gcgcggacct tgcccctcgc gtgattccga aggatcactc gttcgtgacg
     3541 cctcgacgat gtcgttattg cactgcgatg ttcgaagcga tgcgacgctc gagttactta
     3601 ctagtcccaa gtcccgctga ataaactatc cagtaaacgg attctgtcaa ggggcgaggg
     3661 gtaactttcc ggcagattgt ggggtcgtga atccgctcac gacccggcgg gttcggcccg
     3721 cgctacgtgc gcgggccgaa cgtgatctga acgagcttgt cgcgcgtgac gtacttgcgc
     3781 acggtggtgc gcacttcgtc ggcggtgatg gcgttgacct cggcggtgag ctcttccgcg
     3841 ctgcggcctt cgcgcagcag gtgcttcata cccacgttca acgcctcctg cccgatcacc
     3901 tggtggtgcg cgagaggata atcgggaacg tcgggcttga gcatggacgc ggtgacgaag
     3961 ctcacgatgc tgcccttgac ggtggcgacc agcgcgtcgt cgtcgagcat cgcgaccgca
     4021 cgatcgacgt ggtcgctgag aatacccgac acctcggaga ccgagcgtcc ggtggcgggt
     4081 tccgcgaaga cgaagaacgg gaggtcggcc acgcggtaga cttggttcga gcagtacagc
     4141 gcgcgacagg cgccgtacac ctcttgcgtc gtgttcatcg actggtgcag gaacgcaccg
     4201 aacatggtga ggatcagccg gtcgcgatcg ctc
//
LOCUS       DASPGK010000407        14015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1269713, whole genome shotgun sequence.
ACCESSION   DASPGK010000407 DASPGK010000000
VERSION     DASPGK010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269713"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VEC56_11165"
     CDS             <1..325
                     /locus_tag="VEC56_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14753.1"
                     /translation="GFTSVFGALGWRVGRSIVDDLRADLGLHVGLTEWIFFEEEQTEV
                     RYELELGTEASVRADYEFASRWHAIAEASYQFTFTYERIELAYVSIGLARSFGAPGWI
                     RSVLQ"
     gene            322..2343
                     /locus_tag="VEC56_11170"
     CDS             322..2343
                     /locus_tag="VEC56_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14754.1"
                     /translation="MRRAVWIGAALIALAANGADARRVVVTRERLEAAAATRLSDALQ
                     LVDAWAPASNDGYTWMPTPRALVLPRSTAWSVVLNGQLLDVTVFDAVHLELVPVSIAE
                     VDSIVFVDQVDRSSVGTAWASTSARIEIYAARAPRGWTVGATAMVGNEAGDPGPYRYT
                     DQATPNVDAIGADASLWLARGARDWYLSLSGAMMQHPYTDPAMRDRTVDALSTLRPGA
                     TAPEASEPVSWFYDPNWPAVLRLSSSARVGVRAGGGWHEGIAAVADARRYFHYSEPFG
                     GEVPTDQRALLGSVAGSFGAGARTRVGYRALASREELTDQDDALAFDYEWISRTSAGA
                     VDLTHERGRARTVLFAGLEKRSVDVPDTLTEDEDTFVRAGARVERGFGRANRADVELA
                     ATSDGDDDALSAAARLHWVVRPADTVRVRVALQERLFTENDDLWLWSERGYDVLARQG
                     ESYSIDGPIARTRVLAADAGWSSSGIMGGVALDLGLRRFEDAYVETRAFAYDDTTCSF
                     DAPTRVVTGQSGNVATIETRLYHALGDNSGGDFSWAYVEEFDSDPAFGAMWQTVPRHR
                     LRYTLWARPRPTWAFWARVCHYSPTLWNDYAGVDGATCDVRGVWVTYHSEVGGATFVD
                     AMVQHGMWREQLWVDVIARNVFDADVRYHPAGASLDLTLMVQARLRWRD"
     gene            2331..3158
                     /locus_tag="VEC56_11175"
     CDS             2331..3158
                     /locus_tag="VEC56_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXV14755.1"
                     /translation="MARLMRRGACALLIWIVAWLIALAAPARADECDVSWSSVDDDFA
                     ALFEAPFDMSEESAYVTLGAVTLTGASIIWWDDDIDRMVQRRPDSFPYNAMHQISRLA
                     SWYGESNKNAIITYGVVIGAVALGGVIADDDYVIDTAALMAEAGAFSFVLNVAAKMVI
                     GRARPHTERGPHDFDFLTSPTNSDMHSFLSGHTANAFALAGTAAGRHPHWYVEIPGYT
                     LAVAAGLQRVDSRSHWMSDVVSGAFFGYAIAELLVDRHECDDDAQATAPTVSLTLRF"
     gene            3238..4236
                     /locus_tag="VEC56_11180"
     CDS             3238..4236
                     /locus_tag="VEC56_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HXV14756.1"
                     /translation="MRFTWTVSVCMASAVAASALGCGGSTTFGGAMGGSGGGVGVSTG
                     AVGAGTGGFGMARGKDDHKAAVEEWYRGRIQRLTKEDGYLSLVGLFPLGEGRHRFGSA
                     EDNECVFPASAPAHAGWIVVENGAARLEPDPGARLMIGEERAGAMALATDKDGDPTEI
                     SLGTIKFYVIDRPGTLYLRVKDSESPVRKEFKGIKRYKVDRKWLVPAHLERYDPPKKL
                     TVPNVMGFDEVVDCPGVLVFSLRNQEYRLEPLSESEDDMWIVFGDATNGHDTYGGGRF
                     VYIPAPDAEGNTVIDFNRSYNPPCVFTPFATCPLPTRENVLSVKIEAGEKMWGGAH"
     gene            4293..4862
                     /locus_tag="VEC56_11185"
     CDS             4293..4862
                     /locus_tag="VEC56_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14757.1"
                     /translation="MSAPYGSRFVLAVSAVCLTAVGCYVQSLHSLATDDIAAFDAELV
                     GTWVAEDDEEFVFTFVDTTLGNYTLLCDEGGSQARFDAVLVELGGSTFLDIYPEEPQT
                     ENGFYKDHLMRVHNVLKIERTVDTLWVADFDAEWLSTMVAQKKVAIDHVPLDGAVLLT
                     APTKDLQDLVRKYAKTPEAFSEPVRLRRM"
     gene            5032..6348
                     /locus_tag="VEC56_11190"
     CDS             5032..6348
                     /locus_tag="VEC56_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HXV14758.1"
                     /translation="MTRSIRSSAVLLALVCATESRAQTWVNLTPGAGTAPSARRNASA
                     IWDPVENRMVIFAGTTSGTASVLNDVWGFDLDTQSWVNLTPAAGPPAPAPRLFPASVY
                     DPNGHQMITWSGQTAGNFFNDVWSFDLSGNSWTQFTPTGGPPNIRYGVGYTFDPVARD
                     LVTFAGFTNAGRFDDVWRFNDQAQTWTDVSSAGPGERCLHAACYDAFGHRMIMYGGQN
                     NAGPLGDLWALDLDTDTWTELTALPRPSARFFSSMVYDAANRRATVFGGQGNSGLLNE
                     VWVYDLWTNEWTQLPSTGTPPTPRAGAAGIYDGANDRLIVFGGTDGAVKNEVWALEGL
                     SNTTTGASPSASSALTLHPNHPNPFNPSTTIRFELATRGRAALRIYDVRGALVRTLFD
                     QSRDAGPGTATWDGRDDAGRGVASGVYLYQLSAGGESRTRKMVLLK"
     gene            complement(6352..8004)
                     /locus_tag="VEC56_11195"
     CDS             complement(6352..8004)
                     /locus_tag="VEC56_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14759.1"
                     /translation="MFDAPDIIALTDPGLDNGSKLEVSWELSNDDALVDQYNVYRAAG
                     TLESHDEFRWLRTVPHGTSSIVDSSVVAGVPFVYMVSAAHHGNHGGTGGVNRGIWNDF
                     SAPAQGASTNDFPIIQCYARQSVSGGLDSVDETPAIGCPQSDWDQVVVEILLDPAVFA
                     GVAATAFQLSEPVNETILFHSAGAPITADSSVSVVPPNVEFADGYGRTTFTIHGFSGC
                     GVDSAVVSVYGMQLGYAHLNIKSPDVMLGGVTNGRVDLLDFGTLGASYTSPPKLYNAC
                     VDYEAAAGEINLNDFAAFSAHWGHAAPGGESAPEAASTAVSGTVALEFEESHPLLGEH
                     TLRATMSFEGVPPFKAAMFALRNENPKLEFVGWTELPGYAGETISTETIRDGRREVFI
                     GVFDGDATGGDVTLGTAVFRVVSDQQLVLTDEDLALVTADLLSSTDQRSTLALDAVAV
                     RRSVASVEYKNELAQNYPNPFNPTTTISFSLAQAADASLSIYDVRGALVKLLHNGRAE
                     RGIHRVVWDGSSNSGQKVASGVYFYKLVAGSFTDTKKLTLIK"
     gene            10574..10966
                     /locus_tag="VEC56_11200"
     CDS             10574..10966
                     /locus_tag="VEC56_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14760.1"
                     /translation="MTRTILHILLGALLWVVFGYYWYLVMQQPVTDQTKRTLMIVGSI
                     VAVITIFDALWILHNIRIARFGKRRERRTAAEPPANDFLGRAIIAQSEEAVRAASYIE
                     VHVVEIADANTVVKHKLLRVSELGPEAR"
     gene            10963..12231
                     /locus_tag="VEC56_11205"
     CDS             10963..12231
                     /locus_tag="VEC56_11205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14761.1"
                     /translation="MIDIIGILDAIESNVVYLALLSFFAWYPLISSGVLVLVSMLYHF
                     RRERPINKPEVDPKFTPKVSVVIAAFNEAEHIAESIEGILKIDYPDYEVVIIDDGSTD
                     ETSAKVIPFVRAGQVRLVRKLVNEGKAMALNDGLRCTNGEILLIMDADAVPDPLILRH
                     VVPHFKYPRVGAVTGNPRVYNRDTILSKLQAIEFTAIISLQRRAQRVWGRILTMSGVV
                     GAFHRTALIDVGFYSPDMATEDIDLSWKLQLRFWDIRYEPRAVVWMRVPSTIQGLWHQ
                     RRRWARGLAQVLRRHGMEATTWQRRRLWPLLIESWLSIAWAYCFVVLTALWIFCYGVG
                     YPPVGASPIPNWWGMLIGTMCLVQLLTGVILDRRYDKKIPLYYFTAVFYPLIYWIIMA
                     IVTSISTPGAMMRTPKQSPVRWKPIRGDAL"
     gene            12228..13835
                     /locus_tag="VEC56_11210"
     CDS             12228..13835
                     /locus_tag="VEC56_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14762.1"
                     /translation="MTRAVAVALACASLLGVANADAKRMSRMRAQASDTTRVAIPDPV
                     IAPDLPRAMAVLDSARAAAAADRHKDAIRLYHRAIAFYPPLADDIGVELGNQYTWSDE
                     PDSAMMWYRSHLAKHPDDLDAKIGVARLVSWRDDHEQAERLYDEVLAADSTNLDARLG
                     KAQVINWSGRQRAAAAEYRDILEDHPDELEARAGLMQAQRWMGRPDRAVALADSAPAP
                     ALDAIKTEIEHDAAPGVSYTYERNKDSDDIERRYHTFRGGFSPDLMTRISGVYGHANF
                     EQPGRPEVSRNWFGAVLERQFSEALAMSATAGYQLNSYDRAALGPESFWLDEFNLFVF
                     DAYATLTPRDWTRIDVGVSRGSIDNPDAIFRGISRAELSAGLDQRLRTNLLWISGFES
                     AWYSDDNSSIGLATRVVWEPLWRLPIDLNHRFSSSTGFGYYGFKRTTDGGYYDPRQYL
                     SFHEELAIAMTFSPRVRARLAGRLSLDKENGDDWFWTGRFEASATWAIYRGLGLSAGY
                     TNANSRLDSRPGYEIDGFYVTLDYLFW"
BASE COUNT         2403 a         4667 c         4369 g         2576 t
ORIGIN      
        1 gggcttcacc tcggtgttcg gggcgctcgg ctggcgcgtc ggcaggtcga ttgtcgatga
       61 tcttcgcgcc gaccttgggc tccacgtcgg actcaccgag tggatcttct tcgaagagga
      121 acaaaccgag gtccgctacg agctcgaact cgggacggaa gcgagcgttc gggccgacta
      181 cgagttcgcg tcgcgctggc acgcgatcgc cgaggcgtcc taccagttca ccttcacgta
      241 tgaacgcatc gagctggcct atgtgtcgat cggtctcgcg cgctcgttcg gggcgccggg
      301 gtggattcgg agcgtgctgc aatgaggcgc gccgtgtgga tcggcgccgc gttgatcgcc
      361 ctggcggcca acggggccga cgcgcgtcgc gtcgtcgtga cccgcgagcg cctggaggcc
      421 gcggccgcca cgcgcctctc ggatgcgctg cagctcgtgg acgcgtgggc acccgcgtcg
      481 aacgacggct atacctggat gcccacaccg cgcgcgctgg tcttgccgcg ctcgaccgcg
      541 tggtcggtcg tgctcaacgg gcagttgctc gacgtcaccg tgttcgacgc cgtccatctc
      601 gagttggtgc cggtctcgat cgcggaggtc gactcgatcg tgttcgtcga ccaggtcgat
      661 cgctcgtcgg tcggtacggc gtgggcgtcg acgagtgcgc gcatcgagat ctacgcggcg
      721 cgcgctccgc gcggctggac ggtcggggcg accgcgatgg tgggcaacga ggccggcgac
      781 ccgggcccgt atcgctacac cgaccaagcc acacccaacg tcgacgcgat cggcgccgac
      841 gcttcgctct ggctcgcgcg cggcgcgcgc gattggtacc tgtcgctctc gggcgcgatg
      901 atgcagcacc cctacaccga ccccgcgatg cgcgaccgta ccgtggacgc gctgtccacg
      961 ctgcgacccg gtgccacggc accggaggcg tcggaacccg tctcgtggtt ctacgacccc
     1021 aactggcccg cggtgctgcg actgtcgtcg agcgcgcgtg tgggcgtgcg cgcgggcggc
     1081 gggtggcacg aagggatcgc ggccgtcgcg gacgcgcgcc gctacttcca ctactccgag
     1141 cccttcggcg gcgaagtccc caccgaccag cgcgcgctgc tcgggagcgt cgcggggtca
     1201 ttcggcgcgg gtgcgcgcac gcgcgtcggc tatcgcgcgc tcgcttcgcg cgaggaactc
     1261 accgaccagg acgatgcgct cgccttcgac tacgagtgga tctcgcgcac gagcgcgggc
     1321 gccgtcgatc tcacgcacga gcgcgggcgc gcgcgtacgg tgctcttcgc ggggctggag
     1381 aaacgaagcg tcgacgtgcc cgacacgctc accgaggacg aggacacctt cgtgcgggcg
     1441 ggggcgcgcg tcgagcgcgg gttcggccgc gcgaaccgcg ccgacgtcga gctggccgcc
     1501 acctccgacg gcgacgacga cgcgctctcg gccgcggcgc gcctgcattg ggtggtgcga
     1561 ccggcggaca ccgtgcgcgt gcgcgtggcg ctccaggagc gtttgttcac cgagaacgac
     1621 gacctgtggc tgtggtcgga gcgcggctac gacgtgctcg cgcgccaagg cgagtcctat
     1681 tcgatcgacg gacccatcgc gcgcacgcgc gtcctcgcgg ccgacgcggg ctggtcgtcg
     1741 agcggcatca tgggaggcgt cgctctcgac ctggggctgc ggcgcttcga ggacgcgtat
     1801 gtcgagacgc gcgcgttcgc gtacgacgac acgacgtgct ccttcgatgc gcccacgcgc
     1861 gtcgtcaccg ggcagagcgg caacgtcgcc accatcgaaa cgcgcctcta tcacgcgctc
     1921 ggggacaact ccggcggcga tttctcgtgg gcttatgtag aagagttcga ctccgacccc
     1981 gccttcggcg ccatgtggca gaccgttccg cgccaccgcc tgcgctacac gctgtgggcg
     2041 cgcccgcgtc cgacgtgggc attctgggcc cgcgtgtgtc attattcgcc cacgctatgg
     2101 aacgactacg cgggcgtcga cggtgccacc tgcgacgtcc gcggggtctg ggtcacgtat
     2161 cactccgaag tcggcggggc gactttcgtc gacgcgatgg tgcagcacgg catgtggcga
     2221 gagcagctct gggtcgacgt catcgcgcgc aacgtcttcg acgccgacgt gcgctatcac
     2281 cccgcggggg cgtcgctcga tttgacgctg atggtgcagg cgaggctccg atggcgcgat
     2341 tgatgcgacg cggcgcgtgc gcgctgctca tctggatagt cgcgtggctg atcgcgctcg
     2401 cggcgcccgc gcgcgccgac gagtgcgacg tctcctggtc gtcggtggac gacgacttcg
     2461 cggccctgtt cgaagccccc ttcgatatgt ccgaggagag cgcgtacgtt acgttgggtg
     2521 cggtgacgtt gaccggcgcg tcgatcatct ggtgggacga cgacatcgat cgcatggtgc
     2581 agcggcgtcc cgattcgttc ccgtacaacg ccatgcacca gatctcgcgc ctcgcgtctt
     2641 ggtacggcga gagcaacaag aacgcgatca ttacctacgg tgtcgtgatc ggggcggtgg
     2701 cgctgggcgg tgtcatcgcc gacgacgatt atgtaatcga caccgcggcc ctgatggcgg
     2761 aggcgggcgc gttctcgttc gtgctcaacg tcgcggccaa gatggtcatc gggcgcgcgc
     2821 gtccgcacac cgagcgcggc ccgcacgact tcgactttct cacctctccg accaactccg
     2881 acatgcattc gttcctctcc gggcacacgg ccaatgcgtt cgcgctggcc ggaaccgccg
     2941 ccggccgcca tccccactgg tacgtcgaga ttcccggcta cacgctggcg gtggcggcgg
     3001 gcttgcagcg cgtcgactcg cgcagccact ggatgtccga cgtcgtctcg ggcgcgttct
     3061 tcggctacgc gatcgcggag ctgctggtcg accggcacga gtgcgacgac gacgcgcagg
     3121 ccaccgctcc cacggtctct ctgaccctgc gattctgatc gcggctgggg gcccttgact
     3181 ttcgatgccc ccggtcgcca tcatcgagcc ttcgcgccct accagaagga gattctcgtg
     3241 cgtttcacat ggacggtgtc ggtatgtatg gcgtccgcgg tggcggcttc cgcgctcggg
     3301 tgcggtggat cgaccacctt cggcggcgcg atgggcggga gcgggggcgg cgtcggggtg
     3361 agcaccgggg ccgtgggcgc cggcacgggg ggcttcggca tggcgagggg gaaggacgat
     3421 cacaaggccg ccgtcgagga atggtatcgc gggcgcatcc agcgcttgac caaggaagac
     3481 ggctacctct ccctcgtcgg gctctttccg ctgggagaag gacgccaccg cttcggctcg
     3541 gccgaggaca acgagtgcgt gttccccgcg agtgcgcccg cgcacgcggg ctggatcgtg
     3601 gtcgagaacg gcgcggcgcg gctggagccc gatcccggcg cgcgtctcat gatcggcgag
     3661 gagcgcgcgg gtgccatggc cctcgccacc gacaaggacg gcgaccccac cgagatctcg
     3721 ctcggcacca tcaagttcta cgtgatcgat cgccccggca ctctctacct gcgcgtgaag
     3781 gactccgaga gtccggtgcg caaggagttc aagggtatca agcgctacaa agtcgaccgc
     3841 aagtggctcg tccccgcgca cctcgagcgc tatgaccccc ccaagaagct caccgtgccc
     3901 aacgtgatgg gcttcgacga agtggtggat tgccccggcg tgctggtgtt ctccctgcgc
     3961 aaccaggagt accgcctcga accgctgtcc gagtccgagg atgacatgtg gatcgtgttc
     4021 ggcgacgcca ccaacggcca cgacacctat ggcggcgggc gcttcgtcta cattccggcc
     4081 cccgacgccg agggcaacac ggtgatcgac ttcaaccggt cgtacaaccc gccgtgcgtg
     4141 ttcacgccct tcgcgacctg cccgctgccc acccgcgaga acgtgctatc ggtcaagatc
     4201 gaggcgggcg agaagatgtg gggcggcgcg cactaagccc cttgcgctcg cgcggctcgg
     4261 cgcgctacga tagttgcgga tttcctgccg ttatgtccgc gccgtatgga tctcgatttg
     4321 ttctggccgt ctcggcggtg tgtttgaccg ccgttggatg ctacgtccaa tcgctccatt
     4381 ctctcgccac cgacgacatc gccgcgttcg acgccgagct ggtcggcacg tgggtggccg
     4441 aggacgacga agagttcgtg ttcaccttcg tcgacacgac actgggcaac tacacgctcc
     4501 tgtgcgacga gggcgggtcg caggcgcgct tcgacgcggt tttggtagaa ctaggcggct
     4561 cgaccttcct cgacatctac ccggaagagc cgcagaccga gaacggcttc tataaggatc
     4621 atttgatgcg ggttcataat gtgttgaaga tcgagcgaac cgtggacacc ttgtgggtgg
     4681 cggacttcga cgccgagtgg ctctcgacca tggtcgccca gaagaaggtc gcgatcgacc
     4741 acgtgccgct cgacggggcg gtgctgctaa cggcacccac caaggacctc caggatctgg
     4801 tgcgcaagta cgcgaagacc cccgaggcct tcagcgagcc ggttcgcctg cggaggatgt
     4861 agtttctgcg cgggtcctgt cgccaacagc ccacttgcgc atggccgtaa attcttcgct
     4921 cgcccgtaag gctcccttgc gcgcgcgcga ccaaacgcgc aatccgaacc accccctggg
     4981 ttcgtagatg catcgtcgcc ctggcagctc aacccattcg gaggccatcc catgacgcgt
     5041 tcgatccgct cctcggccgt attgctggca ttggtttgcg cgaccgaatc ccgcgcgcag
     5101 acgtgggtca acctgacccc cggggcgggg accgcccctt ccgcgcgccg caacgcgtcg
     5161 gccatatggg acccggtcga gaaccgtatg gtgatcttcg ccgggacgac gtcggggacc
     5221 gcgagtgtgc tcaacgacgt ctggggcttc gatctcgata cccaatcgtg ggtcaatttg
     5281 acgcccgcgg cgggtccgcc cgctcccgcg ccgcgcttgt tccccgccag cgtctacgac
     5341 cccaacggcc atcagatgat cacctggtcg gggcaaaccg cgggcaattt cttcaacgac
     5401 gtgtggtcct ttgatctatc cggtaactcg tggacgcagt tcacaccgac cggcggtccg
     5461 cccaacattc gctacggcgt gggctacacc ttcgaccccg tcgcgcgcga tctcgtgacg
     5521 ttcgcgggct tcaccaacgc cggtcgcttc gacgacgtgt ggcgcttcaa cgaccaggcg
     5581 cagacgtgga ccgacgtctc gtcggcgggg ccgggcgagc gctgcctcca cgccgcctgc
     5641 tacgacgcat tcgggcaccg catgatcatg tacggcgggc agaacaacgc cggaccgctg
     5701 ggcgacttgt gggcgctcga cctcgacacc gacacctgga ccgaattgac cgcgttgccc
     5761 cgtcccagcg cgcgtttctt ctcgtcgatg gtctacgacg ccgccaaccg ccgcgcgacc
     5821 gtgttcggcg gtcagggcaa ttccgggttg ctcaacgaag tctgggtgta cgacctgtgg
     5881 accaatgaat ggacgcagct tccctcgacc ggaaccccac ccaccccgcg cgccggtgcc
     5941 gccggcatct acgacggcgc caacgatcgc ttgatcgtct tcggcgggac cgacggggcc
     6001 gtgaagaacg aagtgtgggc gctcgagggc ctctccaaca ccacgacggg cgcttcgccc
     6061 tccgcgtcga gcgcactcac cctccatccc aaccacccca atcccttcaa tccctcgacc
     6121 accattcgct tcgagctggc gacgcgcggt cgcgcggcgc tgcgcattta cgacgtgcgc
     6181 ggtgcgctgg tgcgcaccct cttcgaccaa tcgcgcgacg ccggccccgg caccgcaacc
     6241 tgggacggcc gcgacgacgc cggtcgtggc gtcgcttcgg gtgtgtatct ctatcaattg
     6301 agcgcgggcg gcgagtcacg gacgaggaag atggtgttgt tgaagtaggg gctatttgat
     6361 aagggtcagt ttcttcgtat ccgtaaacga cccggcgacc aatttgtaga agtaaacgcc
     6421 gctcgcgacc ttctgaccgc tgttgctcga gccgtcccac acgacgcgat ggatcccgcg
     6481 ctccgctcgc ccattgtgca gcaacttcac cagcgcgcca cgcacgtcat agatcgacag
     6541 gcttgcatcc gccgcttgtg ccagcgagaa cgaaatcgtc gtcgtcgggt tgaacggatt
     6601 cggatagttc tgcgccagct cgttcttgta ctccaccgat gccaccgagc gccgaaccgc
     6661 caccgcatcc aacgccagcg tactccgctg atccgtgctc gataggaggt ctgccgtcac
     6721 caatgcgagg tcctcatcag tcagcaccaa ctgctgatcc gacactacac gaaacaccgc
     6781 ggttccgagt gtgacgtcgc cacctgtcgc gtccccgtca aaaacgccga tgaacacttc
     6841 ccggcgaccg tcccggatgg tctccgtgct aatcgtctcg ccggcgtagc cgggcaactc
     6901 cgtccagcca acgaattcca gtttcggatt ctcgttgcga agagcgaaca tcgccgcctt
     6961 gaacggcggc acaccttcga aactcatcgt cgctcgcagc gtatgctcac ccagcaacgg
     7021 atgcgactcc tcgaactcca acgcgaccgt ccccgaaaca gcggtactcg ccgcttccgg
     7081 cgcggactct ccaccaggtg cggcgtgacc ccagtgtgca gagaaagcag caaaatcatt
     7141 gagattaatc tcaccggcgg cggcctcata gtccacgcac gcgttgtaca gctttggggg
     7201 cgaggtatag ctcgccccaa gagtgccgaa gtccaacagg tccactcgtc cgttcgtgac
     7261 gccgcctagc atcacgtcgg ggctcttgat gttgaggtgg gcgtaaccta actgcatgcc
     7321 atagacactc acgactgccg agtcgacgcc gcacccgctg aaaccatgga tggtgaacgt
     7381 ggttctcccg tatccgtcgg cgaactcaac gtttggcgga acaacgctca cgcttgagtc
     7441 agccgtgatc ggcgcgccgg ccgaatggaa caagatcgtc tcgttgaccg gctcagacaa
     7501 ctgaaaagcc gtcgcagcaa cccccgcgaa taccgccggg tccagcagga tctcaacgac
     7561 aacctgatcc cagtcacttt gaggacagcc gatcgcaggc gtttcgtcca cggaatccag
     7621 gccgccagac acggactgtc gtgcgtagca ttgaataatc ggaaagtcat tggtcgacgc
     7681 gccttgcgcg ggagcgctga agtcgttcca tatcccccgg ttcacaccac ccgttccgcc
     7741 atggtttccg tgatgcgcag cggaaaccat gtaaacgaac ggtacgccgg caacaacgct
     7801 ggagtcgacg atactcgagg taccatgggg cacggtccgg agccagcgga actcgtcgtg
     7861 ggattccagg gttccagcgg cccgatagac gttgtactgg tccaccagcg catcgtcgtt
     7921 cgacaactcc cacgagacct caagcttgct cccgttatcg aggcccgggt cggtcaatgc
     7981 aatgatgtct ggagcgtcga acacaggtct accctccagc caaaaccacc agtcgtagca
     8041 tattggctga tcatcgttcg gcttgagacc atacggatcc cgaagcgccg gaaagccccc
     8101 gggtccgtgg tccaaccaat agtagtagca gtccggctcc gggaataggt agtgaaacac
     8161 tgactcgatc gcaccttcgg gcggcggtgg ttcggaaagg ggcttctcca cgcgtacccg
     8221 gctcgctgga ccccaggggt tatccgcagc gtataaggcc acggaatcgg ctggattcgc
     8281 ggtgtaattg agtccataac aggtcttgag acttgcctgc aactcggcgc ccctgcacag
     8341 gacgtggaag tccgagtacc agaatccggc gaggtcaccg atgctcgtcg cgctacagca
     8401 agtgcacccg taaggctcac cgggtagtgc acgcgagtac gtccccaaca cgacgattac
     8461 tatcacgccg cacaggattc tcgacatgat gccctggcat cctccaaaag gatgaacggc
     8521 gctacggcat tggaaagccc catgcaaact ccagcgtgcc gtctaagagc tgtacaacac
     8581 gccccgagta gtactggtcg ccatcgctcg ttcttgtatg gcttgtcttg gtcccgcaaa
     8641 cctgtataat ccacaccctt tccgttgggc gaattggaac aacttcccca ttaacgaacc
     8701 gggcagggaa ctgtgccgag atccatcgtg gtcgagcgat caattgccca acctgggtcg
     8761 gactcaagcc ttcgttcttc caagccgcgc tcagcgcgtc agccactgtg gacaccatac
     8821 tatcgggaat ctcgggaccc atgttccacc catgagactg caggatttcg tcaagtgatg
     8881 gagattccag gacgggaggt acccacgagt cgctcggatt tgtgtacgct gccaccaagc
     8941 agtttgtctg gtcgtcaatg ataacgttaa gatcaagttg cacgacatcg acctcgttcg
     9001 ataaccgtac gtcggcccga agcaaccaac ctgttccgct tgaaaacggt ccaaaagacg
     9061 cacgatcctc gtactgaaca agcttcgcag tggttacgtc aatggtgctg actccgatga
     9121 ttcctacggc acgcgcgacg actgtcgcag taccaagtcg tgattttgta cccgactcga
     9181 tctcgacgat agaaatcggg ctgcctggcc tggaggcgcc agcaagcgcg ctgacgacgg
     9241 tcacaagtgc ggcgaccccc acaagtcctg cgattggaat cactgccttc tcctgccaac
     9301 acttacatgc gtgcttcgtg cctcagggcg gacacacgtc ttgattcaaa cttcgcctgt
     9361 cccatatcca cacgctaccg ccagctatgc taacatcgcc acatgaccaa gtcgtgagtg
     9421 catacagagt cggcgtagtg tattccagat atccctggtg atggattccc acgttgctga
     9481 tctcacaact attccccggc tcgcccggca cgtgctgatc tgtccgccag gtggaggcgc
     9541 cgacttgaac gtggcacatt gggtagcgat tgaaatcaga ccatggtaga ttcgcataca
     9601 ctagtccata gatcgttatg aaaaggcgat cgccggagcg gaatatcgcg tacgctccgc
     9661 tcaccgcgtt ccctattacg gcctcgggag ggtgatagcc cgaatcggtc ttatactcca
     9721 aatacgcccg gggcacggga ttctgcgggt aacgaagcca tccggtttgg acccagaaag
     9781 cgctgttgta atcaataccg atccagacgt caaactcggc aacctgcttt gtcgggacta
     9841 cttcgatagt cccttgaatt gcatcgatgc cgaagacgga tacctcggtc gcgtgtcctc
     9901 cgcaatagtc gtcgcccagc gctccgccaa ctggccatga cgatgttacg accgcagcta
     9961 tcaagaccca tggaacacgc atcgcggctt cttcttgctc gtctggatct tgtggaaact
    10021 tcacgcttca tctcggcttg ccagttcttc cccacctcac tccccctggc atagctctcc
    10081 gcatcgcgga gactagcgcg tgttctgggt agtgtcaagt atagacttat ctctcgtaaa
    10141 cgcccattag aaatgggctg ggctctctgc tcgtggcatc atccagtttc gttgggcttt
    10201 cggggccgca cgcctcttcc atggatacga atgcgtggcg gtgcggcctg ggccgtgcga
    10261 tagccaacca ctgtcgcgca agcaagtcga tcacccttgg cgcgcaccat gcgtcaaggc
    10321 accgtccccg aaccgccggc gtttccggtt cgaagtagaa gcgttggggc gctatctgcc
    10381 ctatccccga gccgccctgg ggcgaaacgt ggcgcaccga cgcggtcgcc gggtgaccga
    10441 ccccacgcgt cgctccgcga tcgggtagta tccctcccga ttgtcccctt cgaccccgaa
    10501 ttcgctcggg gcatgggcat tgcaattcca gcaaacgcta tccgtatctc tttggaccgg
    10561 tagtcgcttt tcgatgactc gcacgatact tcatattctc ctcggcgcgc tcttgtgggt
    10621 cgtgttcggg tactactggt acctggtcat gcagcagccg gtcacggatc agaccaagcg
    10681 aactttgatg atcgtggggt cgatcgtggc ggtcatcacg atcttcgacg cgctgtggat
    10741 cctccataat attcgcatcg cgcgcttcgg gaagcggcgc gagcgccgca cggcggcgga
    10801 gccgcccgcc aacgacttcc tgggccgcgc catcatcgcg cagagcgagg aggcggtacg
    10861 cgctgcgagc tacatcgaag tccatgtggt cgagatcgcg gacgccaaca cggtggtcaa
    10921 gcacaagctc ctgcgcgtct ccgagctggg gcccgaagcg cgatgatcga catcatcggc
    10981 atcctggacg cgatcgagtc caacgtcgtc tacctggcgc tgctctcgtt tttcgcgtgg
    11041 tacccgctga tctcgagcgg cgtgctggtg ctggtgtcga tgctgtatca cttccgccgc
    11101 gagcgcccga tcaacaagcc cgaggtcgac cccaagttca cgcccaaggt gtcggtcgtc
    11161 atcgccgcct tcaacgaagc cgagcacatc gcggagtcca tcgagggcat cctcaagatc
    11221 gactacccgg actacgaagt cgtcatcatt gacgacggca gcaccgacga gacttcggcc
    11281 aaagtgattc cgttcgtgcg cgcgggccag gtgcgcttgg tgcgcaagct cgtcaacgag
    11341 ggcaaggcga tggcgctgaa cgacggcctg cgctgcacca acggcgagat cctcttgatc
    11401 atggacgcgg atgcggtccc cgatccgttg atccttcgcc acgtggtgcc gcacttcaag
    11461 tacccgcgcg tgggtgcggt caccggcaac ccgcgggtct acaaccgcga caccatcttg
    11521 agcaagctgc aggcgatcga gttcaccgcc atcatctcgc ttcagcgccg cgcgcagcgc
    11581 gtgtggggcc gcattctcac catgagcggt gtcgtgggtg cgttccatcg cactgccttg
    11641 atcgacgtcg gcttctacag ccccgacatg gccaccgagg acatcgatct ctcgtggaag
    11701 ctccagcttc gcttctggga cattcgctac gagccgcgcg cggtggtgtg gatgcgcgtg
    11761 ccctcgacga tccaaggcct gtggcaccag cgccgccgct gggcgcgcgg cttggcgcag
    11821 gtgcttcgcc gccacggcat ggaggcgacg acgtggcagc gccggcgctt gtggccgttg
    11881 ctcatcgaat cgtggctctc gattgcctgg gcgtactgct tcgtcgtgct gacggccctg
    11941 tggatcttct gctacggggt cggttatccg ccggtgggcg cgtcgcccat cccgaactgg
    12001 tggggtatgt tgatcggcac gatgtgcttg gtccagttac tgacgggcgt gattctcgat
    12061 cgtcgctacg acaagaagat cccgctctat tatttcacgg cggtgttcta cccgctgatc
    12121 tactggatca tcatggccat cgtgacgtcg atctcgacgc cgggcgcgat gatgcgcaca
    12181 cccaagcagt cgccggtgcg atggaagccg atccggggtg acgcgctgtg acgcgggcgg
    12241 tcgcggtggc gctcgcgtgc gcgtcgctcc tcggggtcgc caacgcggac gcgaaacgaa
    12301 tgtcgcgcat gcgcgcgcaa gcgtccgata ccacacgcgt cgctattccc gaccccgtga
    12361 tcgcgcccga ccttccgcgc gcgatggcgg tactcgacag cgcgcgcgca gccgccgcgg
    12421 ccgaccgcca caaggatgcc attcgtctct atcaccgcgc gatcgcgttc tacccgccgc
    12481 tcgccgacga catcggcgtg gagttgggca accagtacac gtggtcggac gagccggact
    12541 cggcgatgat gtggtaccgc tcgcacctcg ccaaacaccc cgacgacctc gatgccaaga
    12601 tcggcgtcgc gcgcctggtg agctggcgcg acgaccacga gcaagcggag cgcttgtacg
    12661 acgaagtgct cgccgcggat tcgaccaatc tcgacgcgcg tttgggcaag gcgcaggtga
    12721 tcaactggtc cgggcggcag cgcgcggccg ccgcggagta ccgtgatatt ctcgaggacc
    12781 accccgacga actggaagcg cgcgcgggat tgatgcaagc gcagcgctgg atgggccgcc
    12841 ccgatcgcgc cgtcgctctg gccgactccg cccccgcgcc agcgctggac gcgatcaaga
    12901 ccgagatcga acacgacgcg gcccccggcg tctcgtacac ctacgagcgc aataaggaca
    12961 gcgacgacat cgagcgccgg tatcacacgt ttcgcggcgg cttctcgccc gatctcatga
    13021 cgcgcataag cggcgtctac ggccacgcga acttcgagca gcccgggcgc cccgaggtat
    13081 cgcgcaattg gtttggcgcc gtactcgagc ggcagttttc cgaggcgctc gcgatgagtg
    13141 cgaccgccgg ctaccaactg aactcgtacg accgcgccgc actgggaccc gagagcttct
    13201 ggctcgacga attcaacctg ttcgtcttcg acgcctacgc gacgctcacc ccgcgcgact
    13261 ggacgcgcat cgacgtgggc gtctcgcgcg gctccatcga caacccggac gcgatcttcc
    13321 gcggtatctc gcgcgccgag ctctccgccg gcctcgacca gcgtttgcga acgaaccttt
    13381 tgtggatttc ggggttcgaa tcggcgtggt acagcgacga caacagctcg atcggcctgg
    13441 caacgcgggt ggtctgggag ccgctgtggc gtctgcccat cgatctcaat catcgcttca
    13501 gctcgtcgac cggcttcggc tactacggct tcaagcgcac caccgacggc ggctactacg
    13561 acccgcgcca gtacttgagc ttccacgaag agctggcgat cgcgatgacg ttctcgccgc
    13621 gcgtgcgcgc gcgcctggcc ggccgcctct ccttggacaa ggagaacggc gacgattggt
    13681 tctggaccgg gcgcttcgaa gcctccgcga cctgggccat ctaccgcggc ctgggtctct
    13741 cggcgggtta caccaacgcc aattcgcgac tcgactcgcg ccccggctac gagatcgacg
    13801 gcttctacgt cacgctcgac tacctcttct ggtaacattt ccagtatgac gcgacgtgac
    13861 tgggcgatcg ggttcgcggt gaccgccgtc gcggcgacgg cgttcgtggc cggcatgggc
    13921 ttcttcgtcg cgcggggatt cgattcgagc ggccgcgtgc gcttcgaggc ccccgcgccg
    13981 cgccccgcct ccatggtggc cgaagacggc accgc
//
LOCUS       DASPGK010000408         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_918835, whole genome shotgun sequence.
ACCESSION   DASPGK010000408 DASPGK010000000
VERSION     DASPGK010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918835"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1191)
                     /gene="galE"
                     /locus_tag="VEC56_11215"
     CDS             complement(214..1191)
                     /gene="galE"
                     /locus_tag="VEC56_11215"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXV14763.1"
                     /translation="MKLLVTGGAGYIGSVVAESLVARGHEVCVLDNLTTGHRDAVPSQ
                     ARFVEGDVRDASALGTALGTGTDAVLHFAASSVVAESVARPLDYFDNNVGGSVSLLRA
                     MEKAGVTRMVFSSTAAVYGAPDELPIEEAAPCAPENPYGWSKLMIERVLEASRAAWGL
                     GYVALRYFNAGGATAARGEDHRPESHLIPVVLDAALGTRASVTVFGDDYATRDGTCLR
                     DYIHVSDLADAHVLALDAMASGFSGALNLGSEAGFTVLDVIRATERVTGRSVRYEVGP
                     RRPGDPPALVASSRRASGVLGWRPRASALDEIVRSAHAWRSAHPQGYAR"
     gene            complement(1192..>2005)
                     /locus_tag="VEC56_11220"
     CDS             complement(1192..>2005)
                     /locus_tag="VEC56_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXV14764.1"
                     /translation="GAGGVRGVLMVDQAPIGKTPRSNPVTYVKAFAEVRDVFAAQKKA
                     VKRGYKSGRFSFNVAGGRCARCQGMGFERVEMHFMADLFVRCSECDGKRFNAETLEIA
                     YHGKNLADVLDLTVDDAIGHFAEHRELVDKLGVLSKVGLGYLRLGQPSTTLSGGESQR
                     IKIARELSENVEGGFVYILDEPTTGLHVADVAVLIRVLRELVERGNTVVVVEHNLQVI
                     AQSDWIVDLGPEGGAEGGRVVAEGIPSDVARVEGSYTAEYLRRYLGVRKRRN"
BASE COUNT          322 a          742 c          652 g          289 t
ORIGIN      
        1 ggacacggaa acctcgctac agagtcgtcg agcgggcgga tgccacccgc gaaaggcctc
       61 aaggtctaga gaaatggacg cgcggggagg gtcgcacggc gggtgggaac cccgggtgtg
      121 gttcggcgcc ccacgtcgcg cttcacgaag cccgcgcgcg tgaccgaacc gcaaccactg
      181 taacgaattg cggcgattct gtcaacccat gggctagcgc gcgtagccct gcggatgggc
      241 cgaacgccac gcgtgcgcgg agcgcacgat ctcgtcgagg gcgctggctc ggggccgcca
      301 tcccagcacc cccgaggccc ggcgcgagga cgcgaccagc gccggtgggt cgcccgggcg
      361 tcgcgggccg acctcgtagc ggaccgaccg cccagtgacg cgctcggtgg cgcgaatcac
      421 gtcgagcacg gtgaagcccg cctcgctgcc caggttcaac gcgcccgaga agccgctcgc
      481 catcgcgtcc aacgcgagca cgtgcgcgtc cgccaagtcg gacacgtgga tgtagtcgcg
      541 caggcaggta ccgtcgcgcg tggcgtagtc gtcgccgaac acggtgaccg acgcgcgcgt
      601 gcccagggcc gcgtccagaa cgacgggaat caggtgcgac tcggggcggt ggtcctcgcc
      661 gcgcgcggcc gtggcaccgc cggcgttgaa atagcgcagc gccacgtacc cgagtcccca
      721 cgcggcgcgc gacgcttcga gcacgcgctc gatcatgagc ttgctccacc cgtacgggtt
      781 ctccggcgcg cacggcgccg cttcttcgat cgggagctcg tccggcgccc cgtagacggc
      841 ggcggtcgac gagaagacca tgcgcgtgac gcccgctttc tccatggcgc gcaacagcga
      901 caccgaaccg cccacattgt tgtcgaagta atcgagcggc cgcgccaccg actcggccac
      961 tacactggag gccgcgaagt gcaggaccgc gtcggtgccg gttcccagcg cggtacccag
     1021 cgcggacgcg tctcgcacgt caccttcgac gaagcgcgcc tgcgacggca ccgcgtcgcg
     1081 atgaccggtc gtcaagttgt cgagcacgca gacttcgtgc ccgcgcgcga ccagcgactc
     1141 ggcgaccacc gacccgatgt agccggcgcc cccggtgacg agcagcttca cttagttcct
     1201 ccgcttgcgc acgcccaggt agcgtcgcag atactcggcg gtgtacgaac cctcgacgcg
     1261 ggccacgtcg gacggtatcc cctccgccac cacgcgtccc ccttcggccc cgccctcggg
     1321 ccccaggtcg acgatccaat ccgactgcgc gatcacttgc aaattgtgct ccaccaccac
     1381 caccgtgttg cccctttcga ccaattcccg cagcacccgg atcaatacgg ccacgtcggc
     1441 cacgtgcagc ccggtggtgg gctcgtcgag aatatagacg aagccgccct cgacgttctc
     1501 ggagagctcg cgcgcgatct tgatgcgctg cgactccccg cccgagaggg tggtcgaggg
     1561 ctggccgagg cgcaggtacc ccagccccac cttggacagg acgcccagct tgtccaccag
     1621 ctcgcggtgc tcggcgaagt gcccgatcgc gtcgtcgacc gtgaggtcga ggacgtcggc
     1681 gaggttcttg ccgtggtacg cgatctcgag cgtctccgcg ttgaagcgct tgccgtcgca
     1741 ctcgctgcag cgcacgaaca ggtcggccat gaagtgcatc tcgacgcgct cgaagcccat
     1801 cccctggcag cgcgcgcagc gcccgcccgc cacgttgaag ctgaagcgcc ccgacttgta
     1861 cccgcgcttg accgccttct tctgtgcggc gaacacatcg cgcacctcgg cgaatgcctt
     1921 cacgtatgtc acggggttgg agcgcggcgt cttcccgatc ggcgcctggt ccaccatgag
     1981 cacgccgcgc accccacccg ctccc
//
LOCUS       DASPGK010000409         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1504385, whole genome shotgun sequence.
ACCESSION   DASPGK010000409 DASPGK010000000
VERSION     DASPGK010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504385"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VEC56_11225"
     CDS             complement(<1..426)
                     /locus_tag="VEC56_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXV14765.1"
                     /translation="MKISIFGLGYVGCVSAACFAKNGHTVVGVDVNADKREILAAGRS
                     PVVEKDLDALIAEGVASGRLRTAASAAEAIAHSDVSLVCVGTPSRANASLDLGFVARV
                     CEEIGAALRPKSDAHVVCVRSTMLPGSTEEVVIPALARGF"
     gene            complement(423..1742)
                     /locus_tag="VEC56_11230"
     CDS             complement(423..1742)
                     /locus_tag="VEC56_11230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV14766.1"
                     /translation="MTPVLIVAYFFPPSGAVGIYRSLKFAKYLPEFGWRPIVLTTANG
                     KFPTYDASLLRMIPEGVPVHRARGVEFLNEGFDRPQGAPPAKTLRSRIHTRIYLAWHA
                     VAVPDTRVGWVWNAYRHAKRLVRSEGIRHVYISGYPFSSFLIGAMLKRDRQLRVVIDY
                     RDPWTQSVTYEHKGLKRWVDRRLEAWLVRRADAVISNTRHNDARMAAEFGVGQPREKF
                     VAIHNGFDAEEFASVAREPNPKFTMTYAGAFYYSVGSDYSGPLGDAMKTYSPLYFFEA
                     LEAFFVRRPEAKTRMRVRFMGVLGQGYDPAIRSHGLEGVIERLGYIDYDEHLRVLKSS
                     DALLLVLSRGEKSRGWIPSKFFQYLGTGNPILAMTPAGEVREIVQETGAGVCVEPDDV
                     AGASRAIEGLFDAWRSGAPAQAPDAGAVSRYERRNLTHALARVLERS"
     gene            complement(1747..>2421)
                     /locus_tag="VEC56_11235"
     CDS             complement(1747..>2421)
                     /locus_tag="VEC56_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14767.1"
                     /translation="YLGFGAAPGAIGVGNRRKIQGAGGVQMEHTRALVVSHGTAVVLD
                     SIRARDESPVSPHTHLARICFRTPTPPGTASADGTRAGIADPRRFTRVVEAFSDQRAR
                     VDVVDDPTDRNCWYSRWYGDLQRGVAVRVSAEFESRVVVASVIRGADVSVVPVRLDAA
                     DTLIAVESHGRRRLIRVRFDPFSIDVGGRVLVGRGAQAASFAEARASASRTTSTPDWL
                     DELTTG"
BASE COUNT          415 a          874 c          782 g          350 t
ORIGIN      
        1 gaagccgcgc gcgagcgccg gtatcacgac ctcctcggtg ctgcccggca gcatcgtgct
       61 gcgcacgcac accacgtgcg catccgactt cggccgcagc gccgccccga tttcttcgca
      121 cacgcgcgcg acgaagccaa ggtcgaggct cgcgttggca cggctcggcg tccccacgca
      181 cacgagcgag acgtcgctgt gcgcgatcgc ttcggccgcc gacgccgcgg tgcgaagccg
      241 cccgctcgcg acgccctccg cgatcagggc gtcgaggtcc ttctcgacca cgggtgagcg
      301 gcccgcggcc agaatttcgc gcttgtcggc gttgacgtcg acaccgacca cggtgtggcc
      361 gttcttggcg aagcacgccg ccgaaacgca gcccacgtat cccagcccga aaatgctgat
      421 cttcatgatc gctccagaac gcgcgccagc gcgtgcgtca ggttacgccg ttcgtaccgc
      481 gataccgccc cggcgtcggg ggcttgcgcg ggtgcgcccg agcgccaggc gtcgaagagc
      541 ccttcgatcg cgcgcgaagc gccggcgacg tcgtccggct cgacgcacac gcccgcgccc
      601 gtctcctgga cgatctcgcg tacctcgccc gcgggcgtca tggccaagat ggggttcccg
      661 gtccccagat actggaagaa tttggacggg atccagcccc ggctcttctc gccccgcgac
      721 agcacgagca gcagggcgtc cgagctcttc aacacccgca agtgctcgtc gtagtcgatg
      781 tatccgagcc gctcgatcac accctccagt ccgtgcgacc gaatcgccgg atcgtacccc
      841 tgacccagca cgcccatgaa gcgcacgcgc atccgcgtct tggcctccgg ccgacgcacg
      901 aagaacgcct ccagcgcctc gaagaaatac agcggcgaat aggtcttcat cgcatcgccg
      961 agcggacccg agtagtccga ccccacactg tagtagaaag cgccggcgta ggtcatcgtg
     1021 aacttcgggt tcggctcgcg cgcgacggac gcgaactcct cggcgtcgaa cccgttgtga
     1081 atggcgacga acttctcgcg cggctggccg accccgaact cagccgccat gcgcgcgtcg
     1141 ttatggcgcg tgttggagat tacggcgtcc gcccggcgga ccagccacgc ttccagcctg
     1201 cggtcgaccc agcgcttcaa tcccttgtgt tcgtaggtca cgctttgcgt ccaaggatcg
     1261 cgatagtcga tcacgacgcg aagctgacga tcgcgcttga gcatggcgcc gatcaggaac
     1321 gacgaaaacg ggtagccgga aatgtacacg tgccgtatgc cctcgctgcg aacgagccgc
     1381 tttgcgtgcc gatacgcgtt ccacacccag cccacgcgcg tgtcgggaac cgcgaccgcg
     1441 tgccacgcga ggtagatgcg ggtatggatg cgcgagcgca gcgtttttgc cggcggcgcc
     1501 ccttgcggcc ggtcgaagcc ctcgttgagg aactcgacgc cgcgcgcgcg atgcaccggc
     1561 acgccctccg ggatcatgcg caggagcgac gcgtcatagg tcgggaactt gccgttcgcc
     1621 gtggtcagca cgatcggacg ccatccgaac tccggcagat acttcgcgaa cttgagcgaa
     1681 cgatagatgc cgaccgcgcc cgaaggtgga aagaagtatg caacgatgag caccggcgtc
     1741 atgggttcaa cccgtcgtca gctcgtccag ccaatccggc gtcgacgtgg tgcgcgacgc
     1801 ggacgcgcgc gcctcggcga aggacgcggc ctgggcgccg cggcccacca ggacccgccc
     1861 gcccacatcg atcgaaaacg gatcgaagcg cacgcgaatc aagcggcgcc ggccgtgcga
     1921 ctcgacggcg atcagcgtgt ccgccgcgtc caagcgtacc ggcaccaccg agacatcggc
     1981 gccgcgaatc accgaagcga ccacgacgcg cgactcgaac tcggcgctta cgcgcaccgc
     2041 gacgccgcgc tgcaggtcgc cgtaccagcg cgaataccag caattgcgat cggtggggtc
     2101 atcgacgacg tccacgcgcg cacgctgatc ggagaaggct tccacgacgc gggtgaagcg
     2161 gcgcgggtcg gcgatgcccg cgcgggtgcc gtcggcgctc gccgtcccgg gcggcgtcgg
     2221 cgtgcgaaag cagatgcgcg cgaggtgcgt gtgcggcgag acgggcgact cgtcgcgcgc
     2281 ccgaatcgaa tccagcacca ccgcggtgcc gtgcgaaacc acgagcgcgc gcgtgtgttc
     2341 catctgcacg ccccccgcgc cctggatctt gcgccggttc ccgaccccga tcgcgcccgg
     2401 cgccgcgccg aatcccagat a
//
LOCUS       DASPGK010000410         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1504449, whole genome shotgun sequence.
ACCESSION   DASPGK010000410 DASPGK010000000
VERSION     DASPGK010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504449"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VEC56_11240"
     CDS             <1..621
                     /locus_tag="VEC56_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14768.1"
                     /translation="PAGISRTSKSVWVPITDLERLTGRRDRVSRFVLDVAPGAGVDSM
                     VAALNTAQLGYTAYRADDVAQRSSRTFVVISNFHKAISFLSILAGSAFLAAIVLLQVQ
                     ELRKSLGVLRVIGFSRTRIYATVVGETVLLANVGATIGVLLALVVSRGVNAHYQRFFD
                     TALVFSSVTGSHVALAYGVATLVGVAVGTLATTYLFKLRVSEVLGR"
     gene            618..1847
                     /locus_tag="VEC56_11245"
     CDS             618..1847
                     /locus_tag="VEC56_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXV14769.1"
                     /translation="MMARVAIRNVLRHRLRTAFTVLALAIAVALLADMLMLSRGMEKT
                     FYRVLSSVGYEVRVCPLGTLPFATDAVIVGSSRVTHALDDDARVERWLRILATTLYAD
                     STSLFAMGIDGGAQSLYTVTRGADIDTPRADDLVPILLNENAAAALRADVGDAIDLGA
                     EVPGLTSAFVRGGAERPAAFVVAGIIDIEFDLAQQRTLVLPLSALYRLRPETRDAASF
                     VLVKLRAPGEADPAFAPLEEQRAVAASLAAQFPELSTYSIDVLMEALQGQLAYFKQFA
                     LILSTISVFITFLLIAVVLTIGVGERRGEIAALRSMGFRRSSVRGMVLGESVVLLALG
                     AVVGSVLGWLLAGYLDRILTQSPSLPAGYRFFIPAPREIALAVLITGIAGAAAALLPA
                     VFAARVDIPRTLHEEVV"
     gene            1848..2516
                     /locus_tag="VEC56_11250"
     CDS             1848..2516
                     /locus_tag="VEC56_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14770.1"
                     /translation="MSDLVIRASGVGKTYRVGASVIEAVRAFDLAVKPGEFVALMGPS
                     GCGKSTLLNLFGCIDTPSSGRLEVFGADVAAMTEAQRASVRLRRIGFVFQRFYLLPVL
                     TALENVELPMIEARVAKAERRARAGALLTRVGLSHRVHHRPYQLSGGEMQRVAIARAL
                     SNRPDLLIADEPTGELDRKTGASILGLIQELRSDGLAIVMATHDAHAAQLADRIVDME
                     RLAT"
     gene            2513..>2973
                     /locus_tag="VEC56_11255"
     CDS             2513..>2973
                     /locus_tag="VEC56_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14771.1"
                     /translation="MNTLSFIGKGLRLRPQRTLFLLLGFAVGVMVMTVLLSVGTAVLE
                     QALDEDLTGGGDIVLLPFGIDVSVLKTGGVTSMHFDIPNARFLTRELLSDRRLKDVEV
                     VSPEITRAYATLTHGGREQLVRVDGVVPSRFRALWPAMTASFPDGAGERDY"
BASE COUNT          399 a         1048 c          991 g          535 t
ORIGIN      
        1 ccggcgggca tcagtcgcac gagcaagtcg gtgtgggttc cgatcaccga cctcgagcgt
       61 ctcaccggcc gtcgcgaccg cgtgagccgc ttcgtgctcg acgtcgcccc cggcgccggc
      121 gtggactcga tggtggcggc gttgaacacc gcgcagctcg gatacaccgc gtaccgcgcg
      181 gacgatgtcg cccagcgttc ctcgcgaacc ttcgtcgtga tctcgaactt ccacaaggcg
      241 atctcgttcc tgtccatctt ggcgggctcg gccttcctcg ccgcgatcgt gctcctgcaa
      301 gtccaggagc tgcgcaagtc gctcggcgtc ctgcgcgtga tcggcttctc gcgcacacgc
      361 atatacgcga ccgtggtggg cgagaccgtg ctcctggcca acgtcggagc gaccatcggt
      421 gtgctgctcg cgctcgtcgt ctcgcgcggc gtcaacgcgc actaccagcg cttcttcgac
      481 accgctctcg ttttctcgag cgtcacgggc tcgcacgtgg cgctcgcgta cggcgtggcc
      541 actctcgttg gcgtcgccgt cggcacgctg gcgacgacgt acctcttcaa gctgcgcgtc
      601 agcgaggtgc tggggcgatg atggcacgcg tggcgattcg caacgtcctg cggcaccgtt
      661 tgcgcaccgc attcacggtg ctcgcactcg cgatcgcggt ggcgctgctc gccgacatgc
      721 tgatgctctc gcgcgggatg gagaagacgt tctaccgcgt gttgtcgtcg gtgggttacg
      781 aagttcgcgt gtgcccgttg gggactcttc ccttcgcgac cgacgcggtg atcgtcggaa
      841 gctcgcgcgt cacccacgcc ctcgacgacg acgcgcgcgt cgagcgctgg ctgcgcatcc
      901 tggccacgac actctacgcc gactccacct ccctgttcgc gatgggcatc gacggcggcg
      961 cccagtccct gtataccgtc acgcgcggcg ccgacatcga cacgcctcgc gccgacgacc
     1021 tcgtgcccat tctattgaac gagaatgccg cggcggcgct gagagcggat gtgggcgacg
     1081 cgatcgatct gggcgccgaa gtacccgggt tgacgagcgc gttcgtaagg ggtggcgccg
     1141 aacgtcccgc ggcgttcgtc gtcgccggca tcatcgacat cgagttcgac ctcgcgcagc
     1201 agcgcacgct cgtgctgccg ctctccgcgc tgtaccgcct gcgccccgag acccgcgacg
     1261 ccgcgtcgtt cgtcttggtg aaactccgcg ctcccggcga ggccgatcca gccttcgccc
     1321 ccctcgagga gcagcgcgcc gtggccgcgt cgttggccgc gcagtttccc gagctatcga
     1381 cgtactccat cgacgtcttg atggaagcgc tgcaagggca gctcgcgtac ttcaaacagt
     1441 tcgcgctgat cctttcgacc atcagcgtgt tcatcacgtt cctcctgatc gcggtcgtgc
     1501 tgaccatcgg cgtgggcgag cgacgcggcg aaatcgcggc gctgcgcagc atgggcttcc
     1561 ggcgctcgtc ggtgcgcggg atggtgctgg gcgaaagtgt ggtgctgctc gcgctgggtg
     1621 cggtggtcgg ctcggtgctg gggtggctcc tcgcgggcta cctcgaccgc atcctcaccc
     1681 aatcgcccag cctccccgcc ggctaccgct tcttcattcc cgcgccgcgc gagattgcgc
     1741 tcgcggtgct gatcacgggc atcgccggcg ccgccgcggc actcctgccc gcggtgttcg
     1801 cggcgcgcgt tgacattccc cgcacgctgc acgaggaggt cgtgtaagtg agcgatctcg
     1861 tgattcgggc gagtggagtg ggcaagacct accgcgtggg cgcgtccgtg atcgaagcgg
     1921 tgcgcgcgtt cgacctcgcc gtcaagccgg gcgagttcgt ggccctcatg ggcccgagcg
     1981 ggtgcggcaa gagcacgctg ctcaatctct tcggctgcat cgacacgccc agctccggac
     2041 gcctcgaggt cttcggcgcc gacgtggccg cgatgacgga ggcgcaacgc gcgtcggtgc
     2101 ggctgcgtcg catcggcttc gtgttccagc gcttctactt gctgccggtg ctgacggcgc
     2161 tggagaacgt cgagctgccg atgatcgaag cgcgcgtcgc caaggccgag cgccgcgcgc
     2221 gcgcgggcgc tttattgacg cgcgtcgggc tctcacaccg ggtgcaccac cgcccgtacc
     2281 agctctcggg cggcgagatg cagcgcgtcg ccatcgcgcg cgcgctatcc aaccgccccg
     2341 acctcttgat tgccgacgag ccgacgggcg agctggaccg caagacgggc gcgtcgatcc
     2401 tcggcctcat ccaggagctc cgcagcgacg gcctcgcgat cgtcatggcg acccacgacg
     2461 cgcacgcggc gcagctggcc gaccgtatcg tcgacatgga gcggctggcg acgtgaacac
     2521 cctttcgttc atcggcaagg gactgcgtct gcgaccccag cgcacgctgt tcctcctgct
     2581 cggcttcgcg gtcggcgtca tggtgatgac ggtgctcttg tcggtcggca ccgcggtgct
     2641 cgagcaagcg ctcgacgaag acctgacggg cggcggcgac atcgtgctgc tccccttcgg
     2701 catcgacgtc tcggtcctga agacgggcgg ggtgacgtcg atgcacttcg acattcccaa
     2761 cgcgcgcttt ctgacgcgcg agctgctctc cgaccgacga ctgaaggacg tcgaggtcgt
     2821 ttcgcccgag atcacgcgcg cgtatgccac gctcacgcac ggcggccgcg agcaactggt
     2881 gcgcgtcgac ggtgtcgtac cgtcgcgctt ccgcgcgctg tggcccgcca tgaccgcgtc
     2941 cttcccagac ggggccggcg agcgcgacta cat
//
LOCUS       DASPGK010000411        13756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_216973, whole genome shotgun sequence.
ACCESSION   DASPGK010000411 DASPGK010000000
VERSION     DASPGK010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13756
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216973"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VEC56_11260"
     CDS             <1..364
                     /locus_tag="VEC56_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV14772.1"
                     /translation="WTPSFRELPPTIRFFAGGDNSVRGYDYQSLGPLDDAGYVIGGRL
                     LLTTSAEIQVPIKGKFGLAGFYDAGNAFAHAGEGTMEQGVGSGLRWQSPVGPIRLDLA
                     YAIHHDSWQVHFTMGPDL"
     gene            361..4098
                     /locus_tag="VEC56_11265"
     CDS             361..4098
                     /locus_tag="VEC56_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXV14773.1"
                     /translation="MTRARRITGIAAIGAGALVVLVVLFAAFIVFTTPGAKLALSVAN
                     GRELPVRAVSIDGTLARRFTLYGLEVRTGSVTASIDTVVMAWRPLALRHRRLDLEDVT
                     IAGAGVAITLEPDDSARAVPAPRDTATAPWAVHADRVRVRRASVDAPGDVHLREVEVT
                     ASGGPDGYQADVRVSGSAWRFAEARAFVRASGNSTAATVDSLEARVLGGVVRGHAFVR
                     WDPALSWRARLAGDSLQVGELADVPEDWMGAVSFRARGTGSHGADSLRVGLDLESLDG
                     SLRGKPLSARARVEVGRGRIEASDASVRWGSARATLEGSMAEVADVRLDAEIPSLAEI
                     LPRAQGSASVRGRLAGSPERIEVDVEARGRGVRAGRVDIPDLDATVDATLDASDYAPQ
                     AADVRRADVRVGDGSLEARGKVSWRAGIEWDAAITMSDFETSTLTPARWNLYGPVSMR
                     ATTAGHRRARDLQGSLKLESLSGTLRGHALSGAGNIAFDGDAVDVAALRVAWGDAHVV
                     ADGHAGDTIDIDFDVTAPNLAVIDSSLAGAVSLKGSMRGTRKQPGVTADVAADSVRVG
                     AYAVHQMKGRVDADFAFAAPADVRLLALGVARGGTVLDTVRVDASGPRDAHTASLLVA
                     RKGASAAVTLRGALADSAWSGWIEDVRVHDDMLGDWRTNGRSSVFVSSTAARTDSLVL
                     DSEGARLSARVSWRRGDSTHVAADLRGFELSRLQRRPGRGLKVTGALEATAMAVVDPS
                     GRILARADVGAGPGEIALQDKKVGYRVRASARSGADQVSALVDADVDGAGAKVATIDG
                     SLAIAGFVLGQDSLGAQPIEGRLDVDCRDVGPLLAVLAPSLTNASGSLVAHVTPAGTA
                     GDFRVVGQSRIENARVDLPSGIRLRDIDISVASDGTGSVELEGGVTSGGGRVAIAATS
                     ARSDRGWLNGTFTAKGERFQAVNQPEAQVFVSPDVDVRLEERTATIGGSMRVPFARIE
                     TAQVPASASSASNDVVFVEDTLATKPTVRVSTRVRVELGDSVTFNGFGLRGRLTGGLQ
                     VADEPGRPTAGTGEIQIIDGKYRAFGNELTIDPGRFVFGGGAIDNPGLDVRAYRGLTT
                     QNVMAASGEMVGVNLRGTLRRPEFSVFSNPPMSESEIMSYLVLGRSTSSGSSGEQSAL
                     TNAALMLGMQQGTNIAGDIGKKLALDEAYLDAGSDVSETSFVAGKYLSPKLYVSYAAG
                     LFEHTNTFRTRYSLSSRWTVQTESGTKASSTDLLYWFERGK"
     gene            4212..6668
                     /locus_tag="VEC56_11270"
     CDS             4212..6668
                     /locus_tag="VEC56_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14774.1"
                     /translation="MHRHARVVLTLLAAVIQPFAAHAQSLVHDWSRGFGDADYQYGID
                     VDVDAAGNVVTVGDYFGTMNFGGGPLTSAGDLDVYVAKFDAAGNHLWSVRFGDADRQL
                     AKSVAIDDSDGSIVVVGIFQGSIDFGGGPLVSAGGFDAFMVKFDAFGNYDWSRSYGDA
                     GDQLAYAVAVDTEKYVVLTGPFTNKVDFGGGALTSAGSNDIYIARFDLNGDLQWADRY
                     GDASAQATYSLATDADGNILATGGFGGTLDFGGAPLVSAGLWDAFVVKLDRGGGHMWS
                     ARFGDGLDQYGIGVTADSQGNVLLVGDFEGTVDFGGGAISSAGWFDAFLVKLDGSGGH
                     LWSQGFGEGNGQFTGGVAVDADDNVSMAGYFEVAIDLGGGPIAGVGWTDGYAARFDAA
                     GNHLWSDGFGAALENQYGIACVTDASGGLVVIGYGSGDFDFGGGTIPSGGAEDAYLVK
                     YSTPAPDIQAIVDVPGDQGGWARLFFTRALSDEVGASPYPVINYHVFRRVDSPALVAR
                     VLNEGREAAPSPELPATLGGAPVLTLEGRAFWRAAAGEKNARASSASAGPSFVVWEAV
                     ATVPARQQPFYIGLVPTLADSGAVVQHSVYFVMAQTTTPWVYYDSAPDSGYSVDNLSP
                     ATPLGLAGTPVVSPAALTLAWDPNGENDLSHYAVYRGNTIGFVPGPGNRIGTPASPLF
                     VDDDWEWDSGFYYKVAALDIHGNESGHAVLSPDEVTGVGVGAAPSASFLAQNHPNPFS
                     ASTHIAFGLREDADVRIRIYDVAGRLVRDLVDGRYAADRHLVSWDGRDDAGRAVAGGV
                     YFYRLDAGAFVETRKLLLMR"
     gene            complement(6665..7486)
                     /locus_tag="VEC56_11275"
     CDS             complement(6665..7486)
                     /locus_tag="VEC56_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXV14775.1"
                     /translation="MRFPFTIRVARRVGILVVGMAFLTVAQTSTAQAPFRWEKTRDAT
                     LLSAGVLLGAGSLVLLAGVDPLTIDEIDRLDANDVNDFDRDSIVPFSDAQTGDAMAVV
                     SYLLPLTTLVREDTNRDWKTIGVMWVEATLLNLGINGVVKSTLLRTRPYAYDPETPLD
                     KKTTSSARLSFYSGHTASAACNCFFVARVLSAYVESPKARVAMWAGAATYPALTGLLR
                     VDSGHHFRTDIITGYVIGAAVGYLVPQLHRIGDDRVSLRPAAVGGSTGLEVQIAF"
     gene            7672..9459
                     /locus_tag="VEC56_11280"
     CDS             7672..9459
                     /locus_tag="VEC56_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14776.1"
                     /translation="MSTQANPLRSLGWALACLLLPLPAVAQWTPGGVSLGGYELNGRG
                     AVSDGAGGVIVVWDDRRDGPYPNLSDVYVQRWDATGVEQWGAGGLGVCTDDWEASQSV
                     IVGDGAGGAVVAWVDSRTTGTGYGIYAQHVTAAGATTWTDQGVLASSAGPFIGNINAV
                     ADGSGGIVVVWMDYRTGNNVVFAQRVDATGTPLWAGDVNVSNVATTHAEPCVLETLDG
                     GVIVAWSSGTSSNRDVHAQRLDATGARVWNSGVATVVCGAVASQQSTQMVSDGSGGAV
                     LTWVDRRFGDNIYAQRINASGAPQWMTDGVEVCMDEYPQSYPLACPDGTGGAFIVWSD
                     QRDQANGGGAPRPGNAPGQIFITEPDLYMQRIDGTGTAMWMTDGIAVSAAAAYQYLGG
                     IISDGAGGAVVAWADDSDNDLNDDIVLQRYNSAGSEVWTPGGVVHATGAGHRNVIGLV
                     SDGGSNTIPVWTTYIETALASAPRAEVAAQISSVYVQRVDNTSGNCTCGPSGVGAQRT
                     SRLILTEAVPNPSAGAVELGLFLEREHELAVDVFDVRGRRVLAQRPGPASAGWHALRL
                     DARLLPSGVYFYRVTAGADTGTRKIVIQH"
     gene            9599..12292
                     /locus_tag="VEC56_11285"
     CDS             9599..12292
                     /locus_tag="VEC56_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV14777.1"
                     /translation="MTLTPGSRLGPYEILSALGAGGMGEVYRARDTRLGRDVAVKVLP
                     KHLSSNPEVRIRFEREAKTVSSLNHPHICTLFDVGRERDTDFLVMELVEGETLATRLS
                     KGALPTAELLKLGSEIADALDRAHRAGVIHRDLKPGNVMLTRNGAKLMDFGLARVTGL
                     AGPAGSGVSIGTLTQSPTVAHPLTAEGTLVGTFQYMAPEQLEGKEADARSDLWAMGCV
                     LYEMATGRRAFEGKSQASLITSIMGAQPAPVSQVAPATPAGLDRLVQTCLAKDPADRV
                     QSAHDVKLQLLWMADSGSQAGAPRPTIAGRRRSRDVLAWVVAAVAVAIATGAVVLSKR
                     PVTGSPPVYAYLQPPRGVLFSSSTDRPLPLAVSPDGSMLAFCARDGEGSDLLWVRSLG
                     NDDARPIPGTEGAQGPFFSPDGRSLGFFTASELKRVDVAGGPVITLARGIDQRGGTWN
                     RDGLILFTGDSYGPVSAVPADGGPVTTATALDSIQGETTHRYPCFLPDGRHFLYLARR
                     AGAGAGENPTIYVGQIGSDTRQAVLEVASNVAYASGHLIYVRGGVLVAQRFDPGSLVV
                     DGPAVPLVDDARMDTRFSRGVFAASQNGVLICMTGRDQTRTQLRWLDRAGNSLADVGE
                     PADYTYGGTPRISPDGKGAVMPIANAERGVSDVWIVDLETGRRRKVTVDTADHPAAQW
                     LPDSRSVVVSTNTTRGQNSIDAIQSDGTQRTILRKDDFLWPESIAGGRMLFSFETPGN
                     QGGILLASLSGESPPEPFIDTPANEQGAQFSPDGRFVAYVSDETGRNEVFVAAFPQPG
                     GRWQASQSGGMEPRWNRNGRELFFVDPNNYIVSLELEMDASGFRAGAARQLFQWHGAA
                     GQWRYDVAPDGNRFLVAAALEEDLARPVTIVTDWTRKLENR"
     gene            12303..13151
                     /locus_tag="VEC56_11290"
     CDS             12303..13151
                     /locus_tag="VEC56_11290"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXV14778.1"
                     /translation="MASRVQNEKAAALLALHGGGRLLILPNVWDPLGARILQAKGYPA
                     VATASAAISASLGFQDGERIKRTTLMEAVARIARSVDVPVSADIEAGYARTPSELEDT
                     IRAVIGAGIVGINIEDGLGDGGALRAVDEQCARIAAVREVAAREGLHLVINARIDSFY
                     VSSAFSNSADATEDAVARAAAYAKAGADCVYPLGPGDEKTVRTLRARIVPPINILGSP
                     NAAPLSVLESIGVNRVSFGPYVQRALLRKFADIADGLLNEKDVSGLKGMLTRNEVHEY
                     LLDGPE"
     gene            13170..>13756
                     /locus_tag="VEC56_11295"
     CDS             13170..>13756
                     /locus_tag="VEC56_11295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV14779.1"
                     /translation="MTLAPGTRLGPYEILSPLGAGGMGEVYRARDTRLGREVAVKVLP
                     RHLATNAEARVRFEREASAVAALKHPNIVTIHSIEESAGVHFMVMELVEGRTLADVVS
                     EGQLSLDRFLEMAGPLADGVAAAHAKGIIHRDLKPANILVDANGRVKILDFGLVKFSE
                     RSFSDSDLTVGSDSQTQDGQILGTVAYMSPEQAEG"
BASE COUNT         2119 a         4576 c         4890 g         2171 t
ORIGIN      
        1 gtggacgccg tcgtttcgcg agctgccccc gacgatccgc ttctttgcgg gcggtgacaa
       61 cagcgtgcgc gggtacgact accagtcctt gggcccgctc gacgacgcgg gctacgtgat
      121 cggcggtcgc ttgctgctca cgacgtccgc ggagatccag gtgccgatca agggtaagtt
      181 cggtctggcc ggtttctacg acgcgggcaa cgccttcgcg cacgcgggcg aaggcacgat
      241 ggagcagggc gtgggctccg ggctgcgctg gcagtcgccg gtcgggccga ttcgcctcga
      301 cctcgcgtac gccatccacc acgactcgtg gcaggtccac ttcacgatgg ggcccgacct
      361 atgacgcgcg cgcggcgcat taccggcatc gcggcaatcg gcgcgggcgc gctggtcgta
      421 ctcgtcgtgt tgttcgcggc gttcatcgtt ttcacgacac cgggcgcgaa gctcgcgctc
      481 tcggtggcga acgggcgcga gctgccggtg cgtgcggtgt cgatcgacgg tacgctcgcg
      541 cgccgcttca cgctttacgg cctcgaggta cgcaccggat cggtgaccgc atcgatcgac
      601 accgtggtga tggcgtggcg tccactcgcg ttgcggcatc gccgcctcga cctcgaagac
      661 gtgaccatcg ccggggcggg cgtcgccatc acgctggagc cggacgacag cgcgcgcgcc
      721 gtccccgctc cgcgcgacac cgccacggcc ccgtgggccg tgcacgccga ccgcgtgcgc
      781 gtacggcgcg cgtcggtcga cgcccctggc gacgtgcatc tgcgcgaggt ggaggttacc
      841 gcgagcggcg gacccgacgg ctaccaggcc gacgtccgcg tttccggttc ggcgtggcga
      901 ttcgcggagg cacgcgcgtt cgtgcgcgcg tcggggaact cgaccgccgc caccgtcgac
      961 tcgctcgagg cgcgcgtact gggcggtgtg gttcgcggcc acgcgttcgt gcgctgggat
     1021 ccggcgctct cgtggcgcgc gcggctggcc ggcgactccc tccaggtcgg cgagctggcc
     1081 gacgtccccg aggactggat gggtgcggtc tcgttccgcg cccgcggcac cggctcgcac
     1141 ggcgcggact cgctgcgcgt cggactcgac ctcgaatccc tggatggttc gctgcgcggg
     1201 aagccgcttt cggcgcgcgc gcgcgtcgag gtggggcgcg gccgcatcga ggcgtccgac
     1261 gcgtcggtgc ggtggggaag cgcgcgcgcc acactcgaag gcagcatggc ggaggtcgcc
     1321 gacgtgcggc tcgacgccga gattccgtcg ctcgcggaga tcttgccgcg cgcgcagggg
     1381 tcggcgtcgg tgcgcggaag gctggccggc tcgccggagc gcatcgaggt cgacgtcgaa
     1441 gcgcgcgggc gcggggtgcg cgccggccgc gtcgacatcc ccgacctcga cgccaccgtc
     1501 gacgccaccc tggatgccag cgactacgcg ccgcaggccg ccgacgtgcg ccgcgccgat
     1561 gttcgcgtgg gcgacgggtc gctcgaggcg cgcgggaagg tctcgtggcg cgccggcatc
     1621 gaatgggacg ccgccattac gatgagcgac ttcgagacct ccacgctcac acccgcgcgc
     1681 tggaacctct acgggccggt gtcgatgcgc gcgacgacgg cgggacaccg gcgagcccgc
     1741 gacctgcagg gcagtctgaa actcgagtcg ctctcgggga cgctgcgcgg ccacgccctc
     1801 tccggcgcgg ggaacatcgc gttcgacggc gacgcggtgg acgtcgcggc gctgcgcgtc
     1861 gcatggggtg acgctcacgt ggtcgcggac ggccacgccg gcgacacgat cgatatcgat
     1921 ttcgacgtga cggcgccgaa cctggcggtc atcgactcgt cgctcgcggg cgccgtctcg
     1981 ctcaagggtt cgatgcgcgg gacgcgcaag cagcccggtg tgaccgccga cgtcgccgcc
     2041 gacagcgtgc gcgtaggcgc gtacgccgtg caccagatga agggccgcgt cgatgccgat
     2101 ttcgcattcg ccgcacccgc cgacgtgaga ctgctcgccc tgggggtcgc gcgcggcggg
     2161 accgtgctcg acacggtgcg cgtcgacgcc tccggcccgc gcgacgctca caccgcctca
     2221 ctcttggtcg cgcgcaaggg cgcgagcgcc gccgtgacgc tgcgcggggc gctcgcggac
     2281 tcggcgtggt cgggatggat cgaagacgtc cgcgtgcacg acgacatgct gggtgactgg
     2341 cgtaccaacg ggcgctcgag cgtgtttgtc tcgagcaccg cggcgcgcac ggactccctc
     2401 gtgctcgatt ccgagggcgc gcgcctttcg gcgcgcgttt catggcggcg cggcgacagc
     2461 actcacgtcg ccgcggacct gcgcggtttc gagctgtcgc gattgcagcg ccgccccggg
     2521 cgggggctca aggtcacggg cgcgctggag gcgaccgcga tggcggtcgt cgacccgtcg
     2581 gggcgcatcc tcgcgcgcgc cgatgtgggc gcgggtccgg gggagatcgc gctgcaagac
     2641 aagaaggtcg gctaccgcgt gcgcgcgagt gcgcgctccg gcgcggacca ggtctccgcg
     2701 ctcgtcgacg ccgacgtgga cggggccggc gccaaagtgg cgacgatcga cggcagcctg
     2761 gcgatcgcgg gcttcgtcct cggccaggac tcgttgggcg cgcagccgat cgagggccgg
     2821 ctcgacgtcg actgccgcga cgtcggcccg ctgctcgccg tgcttgcccc ctcgctcacc
     2881 aacgcgtcgg ggtcgctcgt cgcgcacgtc acccccgcgg gcacggcggg ggattttcgc
     2941 gtggtcggac aatcgcggat cgagaacgcg cgcgtcgacc tcccctcggg aatccgcttg
     3001 cgcgatatcg acatctccgt cgcgtcggac ggaaccggca gtgtcgagct ggaaggcggc
     3061 gtgacctcgg gcggcgggcg cgtggcgatc gccgccacgt ccgcgcgctc ggaccggggc
     3121 tggctcaacg gtaccttcac cgccaagggc gagcgcttcc aggccgtcaa tcaacccgag
     3181 gcgcaggtgt tcgtctcgcc cgacgtcgac gtgcgtctcg aggagcggac cgcgaccatc
     3241 ggcgggagca tgcgcgtgcc cttcgcgcgc atcgagaccg cgcaagtgcc ggcgtcggcg
     3301 tcgtccgcct cgaacgacgt cgtgttcgtc gaagacacct tggcgaccaa gcccaccgtg
     3361 cgggtgagca cgcgcgtgcg cgtcgagctc ggcgacagcg tcacgttcaa cggcttcggg
     3421 ctgcgcggcc gcttgaccgg cggcctccag gtcgccgacg agcccggccg gccgaccgcc
     3481 gggacgggcg agatccagat catcgacggc aagtaccgcg cgttcggaaa cgagctgacg
     3541 atcgaccccg ggcgcttcgt gttcggcggg ggcgccatcg acaaccccgg cctcgacgtg
     3601 cgcgcctacc gcggcctgac cacgcaaaac gtgatggccg cgtcggggga gatggtgggc
     3661 gtcaacctgc gcgggacgct gcgccgtccc gagttttccg tgttctcgaa tccccccatg
     3721 tcggagagcg agatcatgtc gtacctggtg ctgggacgct cgacgtcctc ggggtcgagc
     3781 ggcgaacaat cggcgctcac caacgccgcg ctgatgctcg gcatgcagca gggcaccaac
     3841 atcgcggggg acatcgggaa gaagctcgcg ctggatgaag cctacctcga cgccggcagc
     3901 gacgtcagcg agacctcctt cgtggcgggc aagtacctct cgcccaagct gtacgtgagc
     3961 tacgccgccg gcctgttcga gcacaccaac acgttccgga cgcggtactc gctctcgtcg
     4021 cggtggaccg tccagaccga gagcggcacc aaggccagca gcaccgacct cctgtactgg
     4081 ttcgagcgcg ggaagtgacg cccgcgaaag cgggcgcaat gacccgcccg cgaaagcggg
     4141 cggagccgcc gtgatttctc gcccccccat ccgtcccatc tgacgtcgaa caccttttta
     4201 ggaggcctcg catgcaccgt cacgcccgcg tcgtactcac gctcctcgcc gccgtcattc
     4261 aaccgttcgc cgcccacgcc cagtccctgg tccacgactg gagccgtggt ttcggggacg
     4321 ccgactacca gtacggaatc gacgtcgatg tcgacgccgc gggtaacgtc gtgaccgtgg
     4381 gggactactt cggcacgatg aatttcgggg gaggccccct cacgagcgcc ggggacctgg
     4441 atgtttacgt ggccaagttc gacgcggcgg gcaatcacct gtggagcgtg cgcttcggag
     4501 acgcggaccg gcagctggcc aaatcggtcg cgatcgacga ctcggacggc agcatcgtgg
     4561 tggtgggcat tttccagggc agcatcgatt tcggcggcgg accgctcgtc agcgcgggtg
     4621 gcttcgatgc gttcatggtc aagttcgacg ccttcgggaa ctacgattgg agccggagct
     4681 acggtgatgc cggcgaccag ctcgcgtacg cggtggcggt ggacaccgag aagtacgtgg
     4741 tcctaaccgg cccgttcacg aacaaggtgg acttcggcgg cggcgcgctc acgagcgcgg
     4801 gctcgaacga catctacatc gcgaggttcg acctcaatgg tgacctccag tgggccgatc
     4861 gctacggcga cgcgtcggcg caggcgacct actctctggc caccgacgcc gacgggaaca
     4921 tcctggccac gggcggattc ggcgggacct tggatttcgg cggcgccccc ctcgtgagcg
     4981 ccggcctgtg ggacgccttc gtcgtcaagc tggaccgcgg cggcggccat atgtggagcg
     5041 cccgcttcgg cgacggcctg gatcagtacg gtatcggcgt caccgcggac agccagggca
     5101 acgtgctgct ggtgggagac ttcgaaggga ccgtggactt cggcggcggc gctatctcga
     5161 gcgcgggttg gttcgacgcg ttcctggtca agctcgacgg ctcgggcggg catctctgga
     5221 gtcagggctt cggcgagggg aacggccagt tcacgggcgg cgtcgccgtg gacgccgacg
     5281 acaacgtgtc gatggcgggg tacttcgagg tcgcgatcga cttgggcggc ggacccatcg
     5341 cgggcgtggg gtggaccgac ggatacgcgg cgcgcttcga cgccgccggc aatcatcttt
     5401 ggagcgacgg cttcggcgcc gcactggaga accagtacgg catcgcgtgt gtgacggacg
     5461 cctccggcgg cctcgtagtg atcgggtacg gctcgggcga cttcgacttc ggcggcggca
     5521 cgataccgag cggaggcgcc gaggacgcgt acctcgtcaa atactccacg cccgcgcccg
     5581 atatccaggc catcgtcgac gtgccgggcg atcagggcgg ttgggcgcgg ctcttcttca
     5641 cgcgcgccct ctccgacgaa gtaggtgcgt cgccctaccc cgtgatcaac taccacgtct
     5701 ttcgccgcgt cgacagtccg gcgctggtcg cgcgcgtgtt gaacgaagga cgcgaggccg
     5761 cgccgtcgcc ggagctgccc gcgacgttgg gcggtgctcc cgttctcacg ctggaggggc
     5821 gcgcgttctg gcgagcagcc gccggggaga agaacgcgcg tgcatcctcg gccagcgccg
     5881 gcccgagctt cgtggtgtgg gaagccgtcg cgaccgtgcc ggcgcgccag cagccgttct
     5941 atatcggcct ggtgcccacg ctcgcggact cgggtgcggt ggtgcagcac tcggtctact
     6001 tcgtcatggc gcagacgacg acgccgtggg tctactacga cagcgctcct gacagcggtt
     6061 actcggtgga caacctctcg ccggcgacac cgttgggcct cgcgggcacg ccggtcgtca
     6121 gcccggccgc gctcacgctg gcgtgggacc ccaacggcga gaacgacctg tcgcactacg
     6181 cggtgtaccg cggcaacacg atcggcttcg tgccggggcc ggggaatcgg atcgggacgc
     6241 ccgcatcgcc gctcttcgtc gacgacgatt gggagtggga cagcggcttc tactataagg
     6301 tggcagcact cgacatccac ggcaacgaga gcgggcacgc ggtgctctcg cccgatgaag
     6361 tcaccggcgt cggcgtcggt gcggcgccgt cggcgagctt cctggcgcag aatcacccca
     6421 atccgttctc cgcgtcgacc cacatcgcgt tcggcctgcg cgaagacgcc gacgtgcgca
     6481 ttcgcatcta cgacgtggcg gggcgcttgg tgcgcgacct ggtggacggg cgctacgccg
     6541 cggaccgcca tctcgtttcc tgggacggtc gcgacgacgc ggggcgcgcc gtcgccgggg
     6601 gcgtgtattt ctaccggctc gacgcggggg cgttcgtcga aacccggaag ctcttgttga
     6661 tgcgctagaa agcgatttgc acttcgaggc ccgtgctgcc gccgacggcg gccgggcgaa
     6721 gcgagacgcg gtcgtctccg atgcgatgga gctgcgggac gaggtagccg accgccgcgc
     6781 cgatcacgta tcccgtgatg atgtcggtgc gaaagtggtg gccggagtcg acgcgcagga
     6841 ggcccgtgag cgcggggtac gtcgccgcac ccgcccacat cgcgaccctg gccttggggc
     6901 tctcgacgta cgcgctcaac acgcgcgcca cgaagaagca attgcacgcg gcagacgcgg
     6961 tgtgccccga gtagaacgac aggcgcgccg acgacgtcgt cttcttgtcg agcggcgtct
     7021 cggggtcgta ggcgtacggg cgcgtgcgca gcagcgtcga cttgaccact ccgttgatcc
     7081 ccaggttgag cagggttgct tccacccaca tgacgccgat cgtcttccag tcgcggttcg
     7141 tatcctcgcg gacgagggtg gtgaggggaa gcaggtagga caccaccgcc atggcgtcgc
     7201 ccgtctgcgc gtcgctgaag gggacgatgg agtcgcggtc gaagtcgttc acgtcattgg
     7261 catcgaggcg atcgatctcg tcgatcgtga gcggatcgac gccggcgagg agaacgaggc
     7321 tccccgcacc gagcagcacg cccgcgctca ggagcgtcgc gtcgcgcgtc ttctcccatc
     7381 ggaagggcgc ttgcgcggtg ctggtctggg ccacggtgag aaacgccatg ccgacgacga
     7441 gaatgcccac gcgtcgcgcg actcggatgg tgaacgggaa ccgcatggtg gggcagcatc
     7501 gtacttcaga tgcggcgttg aggccagcgg gatccgatcg aacggctccg gacgatgcaa
     7561 gacgcgtgcg ctagtttgtc cgcacgacgc gcttcaccca accttcgcct tcggatcgtg
     7621 tattattgta cgaataggcg cattgccccc ctccgcggag attgatatcc catgagtaca
     7681 caagccaatc cccttagatc actgggctgg gccctggcct gcctgctcct gccgctgccc
     7741 gcggtcgcgc agtggacacc aggcggcgtg agcctgggtg gctacgagct gaacggccgt
     7801 ggcgcggtga gcgacggcgc cggcggcgtg atcgtggtgt gggacgaccg tcgggacgga
     7861 ccctacccca atcttagcga cgtatatgtg cagcgctggg acgccaccgg tgtggagcag
     7921 tggggcgcgg gaggcctggg agtctgcacc gacgattggg aggcgagcca gtccgtcatc
     7981 gtcggcgacg gagccggcgg cgcggtggtt gcctgggtgg acagccgcac aacaggaacc
     8041 ggatacggaa tctacgcgca gcacgtgacc gcggcgggtg cgaccacgtg gacggatcag
     8101 ggcgtcctgg ccagcagcgc gggccccttc atcggcaaca tcaacgcggt ggccgacgga
     8161 agcggcggga tcgtggtcgt ttggatggac taccgcaccg ggaacaacgt ggtattcgcg
     8221 cagcgtgtcg atgccaccgg cacaccgctg tgggcgggtg acgtgaacgt gtcgaacgtc
     8281 gccacgacgc acgccgagcc ctgtgtgctc gaaacgctcg acggcggcgt gatcgtagcc
     8341 tggtcttccg gaaccagcag caaccgggat gtgcacgccc agcgcctcga cgcgaccggc
     8401 gcgcgcgtct ggaattccgg cgtggccacg gtggtgtgcg gcgcggttgc cagccagcag
     8461 agcacgcaga tggtcagcga tgggtccggc ggagcggtgc tcacgtgggt ggaccgccgc
     8521 ttcggagaca acatctacgc gcagcgcatc aacgcgagcg gcgctccgca gtggatgacc
     8581 gatggcgttg aggtgtgcat ggatgaatat ccgcagtcgt atcccctggc ctgcccggac
     8641 ggaacaggtg gcgctttcat cgtgtggtcc gaccagcgcg accaggcgaa tggcggcggg
     8701 gcgccgaggc ctgggaacgc gccgggacag atcttcatca cggaacccga tctctacatg
     8761 cagcgcatcg acggaaccgg caccgccatg tggatgacgg acggcatcgc agtttccgcc
     8821 gcggccgcct accagtacct cggcggcatc atctccgacg gcgccggcgg agcggtggtg
     8881 gcgtgggccg acgacagcga taatgacctc aatgacgata tcgtcctgca gcgctacaac
     8941 agtgcgggca gtgaggtatg gacgcccggc ggtgtggtgc acgccaccgg tgccggccac
     9001 cgcaatgtga ttgggctggt cagtgacggg ggtagcaaca cgatccccgt gtggaccacc
     9061 tacatcgaga ccgcgctggc cagcgctccg cgtgccgagg tggcggcgca gatcagcagc
     9121 gtgtacgtgc agcgtgtcga caacacgagc ggcaattgca cctgcggacc gtctggcgtg
     9181 ggcgcgcagc gcacgtcgcg gctgattctc accgaggcag tgcccaaccc gtcggcgggc
     9241 gcggtagaac tgggtctgtt cctggagcgc gagcacgaac tggccgtgga cgtgttcgat
     9301 gtgcgcgggc gccgcgtgtt ggcgcagcgc cccggcccgg catccgccgg atggcacgcg
     9361 cttcgtctcg atgcgcggtt gctgcccagc ggggtgtact tctatcgggt cactgcgggc
     9421 gccgatactg ggacgcgcaa gatcgtcatc cagcactagc cgcgcaggag cggggatagc
     9481 gcggtgtcgg gtgtgccgta aatgacccgg agaatcgaac cggcgtggac aatgcgcggc
     9541 gcctgcgcta acctgtctcc atgaagggct cctaccaact gcgtctgcac gtttcggcat
     9601 gacgctgact ccaggctccc gtctcggtcc atacgaaatc ctaagcgcgc tcggcgcggg
     9661 aggtatgggc gaggtctacc gcgcgcgcga cacgcgcctg ggccgcgacg tggccgtgaa
     9721 ggtgctgccg aagcacctct cgtcgaaccc ggaggtgcgc attcggttcg agcgcgaagc
     9781 gaagacggtg tcgtcgctca accatcccca catttgcacg ctcttcgacg ttggccgcga
     9841 gcgcgacacc gacttcctgg tcatggaact cgtcgaagga gaaacgctcg ccacgcgcct
     9901 ctcgaaggga gcgctgccca ccgcggagct gctcaagctc ggctccgaga tcgccgacgc
     9961 gctcgaccgc gcgcaccgcg ctggcgtcat acaccgcgat ttgaagccgg gcaacgtgat
    10021 gctgacccgg aacggcgcca agctgatgga cttcgggctc gcgcgtgtga cggggctggc
    10081 gggtcccgcc ggcagcggcg tctcgatcgg caccctcacg caatcgccga cggtcgcaca
    10141 cccgctcacc gcggaaggga cgctggtcgg cacgttccag tacatggcgc ccgagcagct
    10201 ggaaggcaaa gaagccgacg cgcgcagcga cctgtgggcg atgggctgcg tgctctacga
    10261 gatggcgacg gggcgacgcg cgttcgaagg caagagccaa gcgtcgttga tcacctcgat
    10321 catgggcgcg cagccggcgc cggtgtcgca ggtcgccccc gcgaccccgg ccgggctgga
    10381 tcgcctggta cagacgtgtc tcgcgaagga tcccgccgac cgcgtgcaaa gcgcgcacga
    10441 cgtgaagctg cagctcttgt ggatggcgga ctccggttcg caagcgggag cgccgaggcc
    10501 cacgatcgcg gggcgccgtc gaagccgcga cgtgctggcg tgggtcgtcg ccgccgtcgc
    10561 cgtggcgatt gcgacgggtg ccgtcgtgtt gtcgaagcgg cccgtgaccg gatcgcctcc
    10621 ggtgtacgcc tacctgcaac cgccgcgcgg cgtgttgttc tcgtccagca cggatcggcc
    10681 gctgcccctc gcggtatcgc ccgacggttc catgctcgcc ttctgtgcgc gcgacggtga
    10741 aggctccgac ctgctctggg tgcgatcact cggcaacgac gacgcgcgcc ccatccccgg
    10801 gaccgaaggc gcgcaggggc cgtttttctc gcccgacgga cgttcgctcg gtttcttcac
    10861 ggccagcgag ctcaagcgcg tcgacgtcgc cggcggcccg gtcatcacac tcgcgcgtgg
    10921 aatcgatcaa cgagggggaa cctggaaccg cgacggcctc atcctgttta ccggtgactc
    10981 gtacggcccc gtgtccgcgg ttcctgcaga cgggggcccc gtaacgactg cgaccgccct
    11041 cgactccata cagggagaga ccacccaccg gtatccgtgc tttcttcccg acggccggca
    11101 ttttctctac ttggcgcggc gcgcgggggc tggggcgggg gagaacccga ccatctacgt
    11161 cggccaaatc ggatccgata cgcgccaggc ggtgctggaa gtggcctcga atgtcgcata
    11221 cgcgtcgggg cacctgatct atgtacgcgg cggcgtgttg gtcgcgcagc gcttcgaccc
    11281 tgggtcactt gtcgtggacg ggccggcggt tcccttggtc gacgacgcac gcatggacac
    11341 gcgcttcagt cgcggcgtat tcgccgcctc gcagaacggc gtcctcatct gcatgacggg
    11401 acgcgatcaa acccgcaccc agctgcgctg gctcgaccgg gccggaaatt cgctggcgga
    11461 cgtcggcgaa cccgcggact acacgtacgg cggtacccct cggatttcgc cggacgggaa
    11521 gggcgccgtc atgccgatcg cgaacgcgga gcgcggcgtt tccgacgttt ggatcgtcga
    11581 tctcgaaacg ggccgccgcc gcaaggtgac cgtcgatacc gcggaccacc ccgcggcgca
    11641 gtggctcccg gacagtcgct cggtcgtggt gagcaccaac acgacgaggg gccagaattc
    11701 catcgatgcc attcaatccg acgggaccca gcggaccatc ttgcgcaaag acgacttcct
    11761 gtggccggaa tcgatcgccg gtggccggat gctcttctcg ttcgaaacgc cgggcaatca
    11821 aggcggaatt ctcctcgcgt cgttatcggg cgagtcgccg ccggaaccgt tcatcgacac
    11881 gccggcgaac gaacagggcg cgcagttctc gcccgacggg cgcttcgtcg cgtacgtctc
    11941 cgacgaaacc gggcgcaacg aggtcttcgt ggccgcgttt ccgcagcccg gcggccgctg
    12001 gcaggcgtcg cagtcgggcg gtatggagcc gcgctggaac cgaaacggtc gcgagctgtt
    12061 cttcgtcgat cccaacaact acatcgtgtc gctcgaactc gagatggacg cgagcggttt
    12121 ccgggccggc gccgcgcgcc aactcttcca atggcacggg gccgcgggtc agtggcggta
    12181 cgacgtcgcg ccggacggca atcgattcct ggtggcggcc gcactcgaag aggatctggc
    12241 ccggccggtg acgatcgtca ccgattggac gcgcaaactc gagaaccggt agcggtgggg
    12301 cgatggcatc gagagtacag aacgaaaaag ccgccgcgct cctcgcgctg cacggcggcg
    12361 gacgccttct gatcctgccc aacgtgtggg atcccctcgg tgcccgcatc ctgcaggcga
    12421 agggctaccc ggccgtcgcc accgcgagtg ccgcgatatc ggcatcgctc gggttccaag
    12481 acggagaaag aatcaagcgc acgacgctca tggaagccgt cgcgcgcatt gcgcgcagtg
    12541 tcgatgtccc cgtgagcgcg gatatcgaag cggggtacgc ccgcacgcca tccgagttgg
    12601 aagacacgat tcgcgcggtc atcggcgccg gtatcgtcgg tatcaacatc gaggacgggc
    12661 tcggcgacgg tggggcgctt cgcgcggtcg acgagcagtg cgcgcgcatc gcggccgtgc
    12721 gggaggttgc cgcccgagag ggcttgcatc tggtgatcaa cgcgcgcatc gacagcttct
    12781 acgtctcgtc cgccttctcg aattctgcgg acgcgaccga agacgcggtc gcgcgcgcgg
    12841 cggcctacgc caaagcgggg gcggactgcg tgtatccctt gggccccggc gacgagaaga
    12901 ccgtgcggac gctgcgggcc cggattgttc caccgatcaa catcctggga tcgccgaacg
    12961 cggcgccgct gtcggtgctg gagtcgatcg gggtcaatcg cgtgagcttc ggtccctacg
    13021 tccagcgtgc gctcctgcgg aagttcgcgg acatcgcgga cggattgctg aacgagaaag
    13081 acgtctcggg tctcaagggt atgttgacga ggaacgaggt ccatgagtat ctgctcgatg
    13141 gccccgagta aggggcgggg acgatatccg tgacgctcgc tcccggcacc cgactcggcc
    13201 catacgaaat cctctcgccc ctgggtgcgg gcggaatggg cgaggtgtat cgcgcgcgcg
    13261 atacgcggct cggtcgcgaa gttgcagtca aagttctccc gcgacatctc gcaaccaacg
    13321 ccgaagcgcg cgtccgcttc gagcgcgagg cgtccgccgt cgcggcgctc aaacatccca
    13381 acatcgtcac tatccattcc atcgaggaga gcgccggcgt ccatttcatg gtgatggagc
    13441 tggtcgaagg gcgcacgctc gccgacgtag tatcggaagg ccagctgtcg ctcgaccgat
    13501 ttctggaaat ggcggggccg ctcgcggacg gcgtggcggc cgcgcacgca aagggcatca
    13561 tccatcgcga cttgaagccc gccaacatcc tcgtggatgc gaacgggcgc gtgaagattc
    13621 tcgacttcgg tttggtgaag ttctccgaga ggtcgttttc cgattcggat ctgaccgtcg
    13681 ggtcggattc gcaaacgcaa gacggtcaga ttctgggcac ggtggcctat atgtcgcccg
    13741 agcaggccga gggcaa
//
LOCUS       DASPGK010000412         9271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1386473, whole genome shotgun sequence.
ACCESSION   DASPGK010000412 DASPGK010000000
VERSION     DASPGK010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386473"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VEC56_11300"
     CDS             <1..702
                     /locus_tag="VEC56_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14780.1"
                     /translation="TGVSAGVFRSTYITYSLTSGDHTPSFGHYPVAVGVSGTFTSAGD
                     TFVVHGGCPLINDFDVVTPTGSAVMAMTYGAPAADNGAVIRKATGNARVTLSGFSFIY
                     IRDDAPEGIMDRVFHLEDILCSLGDCPDWIQIDVPPSAGTNTLGQNYPNPFNPQTTIA
                     FSLKARGRVRIDVFDVSGARVRTLLDETRATGVHTDVRWDGTNALDQPVASGVYFYRI
                     EAGDFSQTRKMVLLK"
     gene            735..3749
                     /locus_tag="VEC56_11305"
     CDS             735..3749
                     /locus_tag="VEC56_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14781.1"
                     /translation="MRSAILIGSLALHLGVGVAEARKPAEAVPTSEAATRARPTDGSL
                     TPDPRFAITAANTTVLASYNFDIGASCNENSWTKVDATAQIAVYWHVDDFAGANVKPG
                     DAYAALSGAKSLWCGTRHTASGPTCGYLALPGYGNRWNQSWRTKDCIAVTGDLDVSFL
                     MQTDSEAAYDATVLEYTTDCSGPFTGWTELDGGIGVWDGIQGPFAHSASYPVAGSPVK
                     VRLRFESDGAWSDEDANYDSHSGPIVIDNLVAEGLALEDFEDEAQNATTSNDWEGFAE
                     AGYGQFLALFSGAVLLQQDACAKNLSCQWAAIQGSSETYACGGFPLQAAVPKGNSDGQ
                     YLQNEVWSPSFALAGTGSVVDLKFTVYRDMTLDALVFYTWGVRTVSPIGCQSAWRSRG
                     YVYFGDQKDYLVNTFPVGDLVPGSAVSMRVRLGVVDQCGVWCGIFGTGACHSHAPLLD
                     KVRVYRVDVSGPVWSTRDIDMFQDTFPADGTDTGIGRADAALSITPAISPTILPGDSA
                     CFVVSDPITAVPVSNPSGLADDLALGGKQCYLWVHVIDFGVPAPPGKSGSVLSGGPPF
                     PFKDTQTADGKTWTRIQCRLRVESTNTFDVDLNDNLFEAGDVIEFFFGATNTNGETSY
                     CSGSALDYVQSDVDLAAESASEFTILPLYGNGFDSLLYVDGLDGRGGQIFWDTAFDSW
                     GLSVDRYDVRGPTSSVSNRPAARVTDVAAQLNDNYDAILWDAGDLTQSLGDGTGSPEK
                     SNDYALVNSFLAGLTARGGVYICGDDFPSGLNAASGASAVVFKSTYITFTLTTGNHKP
                     SFGTSPIGTPVPGGAFDHTFVIFGGCPLINDFDVMAPTGASIAQISYGAPAANNAAVV
                     SKAIGSDIRTMISGFSLVYIRDDEEDGILDRLQHLWDILTYLGIVLIEIDAPPLPFVD
                     RLEQNYPNPFNPQTTIGFSIKESARVRIDVFDVSGARVRSLLDETRAAGSYSDVRWDG
                     TNDTKQPVASGVYFYRLVAGGFRETRKMVLLK"
     gene            3992..6382
                     /locus_tag="VEC56_11310"
     CDS             3992..6382
                     /locus_tag="VEC56_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14782.1"
                     /translation="MGRIRLFGLIAAAVGALGACASDTLSGDHVENQAPTVWLAAGPP
                     EGSTGTYTVQLFWGGWDPDGEISFYEYLVTDNVTGVFDPADTVGVAWSPVYGNDSTFT
                     FSADSLSETPTGQKAVFMRSHTFFIRAVDEDDKRSKEPAYRSFTSRTISPEVRITTPR
                     AELGLTAADMPPISTLVWNATDYIDSDETKQDPDSVQWAMVSTKTHNNSFLDTIKYLR
                     DNPTASDWYPWSWYRAPGDSGKSWTTPPLEFGDYVFAIRAKDEAGAVTPTLVEPVNVR
                     RIKVAPRISGPTLVVTSTLIGSVIAASCDYPLTIVDVPAGVDLAFSLSACAESYGGTV
                     AGYRYGWDILDLNDPDQWEVDYTPFIGAVATTPARAFNFGVHTFTSEVIDNSGFCTRI
                     EIKVNIVRFTGERNLLVIDDYKSDEVVGQSGWAATNGGVPNDSEHDAFWVDMVSNLDI
                     FDPSRDMVATSRDSELPLTTLAGYKSIIFSVFSDLDTRSPSDLPLLYTYIQYRSKRPP
                     ASTPGACSSSGGVQGKVLPNLIGLAMQAGVHVLIAGNHPAQNALSRTASPPVRWPALP
                     LYEFERGSTQTGSSPQDLDNPPGDHAFAYRDLCLDVIDYGFLTSSRARVRGGGSVANQ
                     RYCPINAWRSPNSQSRRDDGMRAGVPADPNFPRIELRPEAAGTGRLFNPASQAIDVEV
                     YNPAYFRQGQACAWVQPPRPCFEPIYLLECLDTAEATYLQPIAFWTTTYADVVSEDIP
                     GAVAARSAVFGFPPVYFNPAQVKPAIEYIMFDEWQLPRRPVGTASQGRLDGTAGAH"
     gene            6526..>9271
                     /gene="metH"
                     /locus_tag="VEC56_11315"
     CDS             6526..>9271
                     /gene="metH"
                     /locus_tag="VEC56_11315"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXV14783.1"
                     /translation="MLSERILILDGAMGTMIQRYQLDEAAFRGDRFRDHPKDLKGDND
                     LLCLTQPKIIGEIHRAYFDAGADVATTNTFNGTSISQHEYGLEAFVYEISREAARIAR
                     AEADAAEGRDPSRPRFVAGSLAPTNRTASISPDVNDPGFRNVTFVQLRDAYRENARGL
                     IDGGADILLVETVFDTLNAKAALFAIEDEFEARGYRLPVICSGTITDLSGRTLSGQTP
                     EAFYASVSHVPLLAVGLNCALGSAQMKPFLAALASVARVPVSVYPNAGLPNAFGGYDE
                     TPDEMAANLRDMADDGWINIAGSCCGSTPEHTRAIAQAMRGVAPRRAPERPRYTLLSG
                     LEPLEIRPDSNFINVGERTNVTGSARFRSLIKENDYEAALTVARQQVEDGAQLIDVNM
                     DEGLLDSEAAMRRFLNIVSTEPAIARVPIMIDSSKFEVIEAGLQCAQGKCVVNSISLK
                     EGEAAFIAHARRVRRYGAAVIVMAFDENGQADTVERKVEICARSYRILTEQLDFPPED
                     IIFDPNVFAIATGIEEHNAYAVHFIEACRELKQRFPLSHVSGGVSNVSFSFRGNEPVR
                     QAIHAVFLYHAIRAGMDMGIVNAGQLMVYEDIPKDLLERVEDVVLNRRPDATERLLEV
                     AESMRRGADPRKKDDEAWRALPVIERLKHALVHGLDAHIVEDVEEARQAAARPIHVIE
                     GPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAWLTPFMEQEKERTGRREAEARI
                     VMATVKGDVHDIGKNIVGVVLACNNYEILDLGVMVSADAILKAARESGADLVGLSGLI
                     TPSLEEMAHVAREMERQEFKLPLLIGGATTSKAHTAVKIAPAYSQPVVHVLDASRAVT
                     VASQLKSAGQREAFAAQNRAEQDALRRQHQDKRSAKPLLTLAEARRRRPRVDWESYEI
                     P"
BASE COUNT         1597 a         3182 c         2925 g         1567 t
ORIGIN      
        1 accggcgtct ccgcaggcgt gttccgctcc acgtacatca cctattcgtt gaccagcggc
       61 gaccacacgc cgagcttcgg ccattatccg gttgccgtcg gcgtcagcgg gacgttcacg
      121 tctgcgggcg acacgttcgt cgtccacggc ggatgtccgc ttatcaacga cttcgacgtc
      181 gtgacgccga cgggctcggc cgtcatggcg atgacctatg gagcgccggc cgcggacaac
      241 ggggccgtga tcagaaaggc gacgggcaat gcgcgcgtga ccctttccgg attcagcttc
      301 atctacattc gcgacgacgc ccccgaaggg atcatggatc gcgtgttcca tttggaagac
      361 atcctgtgtt cgctcggcga ttgcccggat tggatccaga tcgacgtgcc tccctcggcc
      421 ggcaccaaca ccctcgggca gaactacccg aacccattca acccgcaaac cacgatcgcg
      481 ttctcgttga aagctcgtgg gcgcgtaaga atcgacgtgt tcgacgtgtc gggagcgcgc
      541 gtgcggaccc tcctcgatga gacgcgggcg accggcgtgc ataccgacgt gcgctgggac
      601 ggcaccaacg cgctggacca gcccgtcgcg agcggcgtct atttctatcg aatcgaggcc
      661 ggagactttt cccagacgcg gaagatggtg ctattgaaat agcctcgccc cgaccgacca
      721 ctggaggcca tgtcatgcgt agcgcaattc tcattgggtc tctcgcgctg cacctcggtg
      781 tcggtgtcgc cgaagcgcgc aagccagccg aggcggttcc cacatccgag gcagcgacgc
      841 gtgcgaggcc gaccgacggt tccctcactc ccgatccgcg cttcgcgatc acggccgcca
      901 acaccacggt gctcgccagc tacaatttcg acataggcgc gtcgtgcaac gagaacagct
      961 ggacgaaggt cgatgccacc gcgcagatcg cggtgtactg gcacgtcgat gacttcgcgg
     1021 gcgccaacgt caagccgggc gatgcgtacg cggcgctctc cggcgcaaaa tcgctctggt
     1081 gcggcacgcg gcacacggct tcgggaccga cctgcgggta cctggctctc ccgggatacg
     1141 ggaaccgctg gaatcagtcg tggcgcacga aagactgtat tgcggtgacc ggagacctcg
     1201 acgtgtcgtt cctgatgcag accgactcgg aggccgcgta cgacgcgacg gtcctcgagt
     1261 acacgaccga ctgctcgggc ccgtttaccg gttggaccga gctcgacggc ggtatcggcg
     1321 tgtgggacgg catccagggt ccgttcgcgc acagcgcgtc gtatccggtc gccggcagcc
     1381 cggtcaaggt ccgtctgcgc ttcgagtccg acggcgcctg gtcggacgag gacgcgaact
     1441 acgatagcca ctccggacct atcgtcatcg acaacctggt cgccgaagga ctcgcgttgg
     1501 aggacttcga ggacgaggcg cagaacgcga cgacgtcgaa tgactgggaa ggcttcgcgg
     1561 aagcgggcta cggccagttc ttggcgttgt tctcgggcgc ggtccttctg cagcaggacg
     1621 cgtgcgcgaa gaacctctcg tgccagtggg ccgcgatcca gggttcgagc gaaacctacg
     1681 cgtgcggcgg tttcccgctg caggcggcgg ttcccaaggg caacagcgac ggccagtacc
     1741 tccagaacga ggtgtggtcc ccgtcgttcg ccctcgccgg gacgggatcc gtggtcgact
     1801 tgaagttcac cgtgtaccgc gatatgacgc tcgacgctct ggtgttctat acctggggtg
     1861 tgaggacggt cagccccatc ggctgccagt ccgcctggcg gagccgaggc tacgtgtatt
     1921 tcggtgacca gaaggactac ttggtcaaca cgttccccgt gggcgacttg gttcccggat
     1981 cggccgtctc gatgcgcgtc cggctgggcg tcgtcgacca gtgcggcgtg tggtgcggga
     2041 tcttcggcac cggtgcgtgc cactcgcacg ctcccttgct cgacaaggtc cgcgtctatc
     2101 gcgtcgacgt ctcgggaccc gtctggagta cccgcgacat cgacatgttc caggacacct
     2161 tccccgcgga cggtaccgac accggaatcg ggcgcgcgga cgccgccttg agcatcacgc
     2221 cggcgatcag cccgacgatt ttgcccggcg actccgcctg cttcgttgtc agcgacccca
     2281 tcaccgcggt ccccgtcagc aacccgagcg gtctggccga cgacctcgcg ctcggtggca
     2341 agcagtgtta cctctgggtt catgtcatcg acttcggtgt gccggcgccg ccgggcaaat
     2401 ccggctcggt cctctcgggc ggcccgccgt ttccgttcaa ggacacgcag acggccgacg
     2461 gcaagacctg gacgcgtatt cagtgccggc tgcgcgtcga gtcgaccaat accttcgacg
     2521 tcgatttgaa cgacaatctc ttcgaggccg gcgacgtgat cgagttcttc ttcggcgcca
     2581 ccaataccaa cggagagacg agctattgct cgggcagcgc tctcgactac gtccagagcg
     2641 acgtcgatct cgccgcggag agcgcgtcgg aattcacgat tctgcccctc tacggcaacg
     2701 gcttcgactc cttgctctac gtcgacggcc tcgacggccg cggcggacag attttttggg
     2761 acaccgcatt tgattcgtgg ggtttgtcgg tcgatcgcta cgacgtgcgc ggaccgacct
     2821 ccagcgtgag caaccggccg gccgcgcgcg tgaccgacgt cgcggcacaa ctgaacgaca
     2881 actacgacgc gattctgtgg gacgccggcg atcttacgca gagtttgggc gacggcaccg
     2941 ggtccccgga gaagagcaac gactatgcgc tggtcaattc gttcctggcc gggctgacgg
     3001 cacggggcgg cgtttatatc tgcggcgacg actttccctc cggactcaac gcggccagcg
     3061 gcgcctcggc ggtcgtcttc aagagcacct acatcacgtt tacgctgact acgggaaacc
     3121 acaagccgag ctttggcacc tcgcccatcg gcacgcccgt gcccggcgga gcattcgacc
     3181 ataccttcgt gatcttcggg gggtgtccgt tgatcaacga cttcgacgtg atggccccca
     3241 cgggagcgtc catcgcgcag atctcgtacg gggcgcccgc ggccaacaac gccgcggtcg
     3301 tctccaaggc gatcggcagc gacatccgca ccatgatttc ggggttcagc ctcgtctaca
     3361 tccgcgacga cgaggaggac ggtattctgg accgattgca gcacctgtgg gacattctga
     3421 cctatctcgg gatcgtgctg attgaaatcg acgcgccacc gctcccgttc gtcgaccggc
     3481 tcgagcagaa ctatcccaac ccgttcaacc cccagaccac gatcggcttc tcgatcaagg
     3541 agagtgcgcg cgtgcgcatc gacgtgttcg acgtttccgg agcgcgcgtg agatcgctcc
     3601 tcgatgaaac ccgggccgcc gggtcctact cggacgtccg ctgggatgga accaacgaca
     3661 cgaagcaacc cgtcgcgagc ggcgtctact tctatcgact ggtggcgggt ggcttccgcg
     3721 agacccgaaa aatggtcctt ctcaaatagg cgccggggga agaaactacc cacgggcggg
     3781 tgctccatca aactgtttgc agcacccgcc cgtttgcgtt atcgtccggc cgggttatct
     3841 tatgggccgg cgcgggcatc cgtgtaaatg gggtcctcgg tccgactcca taagttgtgg
     3901 aattgtatcc tccccacgac accgctgggc cgttcgcccg agagtgcggc gtttatcccc
     3961 cacgccgaga aagacttgca ggaggctttt ggtgggacgc attcgtctgt ttggtcttat
     4021 agccgcggcc gtgggcgcgc tgggcgcctg cgcgtcggac accctgagcg gcgaccacgt
     4081 cgagaatcag gccccgacgg tctggctggc cgccggcccg cccgagggat ccacgggaac
     4141 ctacaccgtc cagttgttct ggggcggctg ggaccccgac ggcgagattt ccttctacga
     4201 gtacctcgtc accgacaacg tcacgggcgt cttcgacccc gccgataccg tgggcgtcgc
     4261 atggtccccg gtctacggga acgactccac ctttaccttc tcggccgatt cgctctccga
     4321 gacgccgacc ggccagaagg ccgtcttcat gcgctcgcac accttcttca tccgtgcggt
     4381 cgacgaggac gacaagcgct cgaaggagcc cgcgtaccgc tcgttcacgt cgcgcacgat
     4441 ttcgcccgag gtccgcatca cgacgccgcg cgcggagctc ggcttgaccg cggccgacat
     4501 gccgcccatc tccacgctgg tctggaacgc gaccgactat atcgacagcg acgaaaccaa
     4561 acaggacccc gactccgtgc agtgggccat ggtgagcacg aagacgcata acaacagctt
     4621 cctcgacacc atcaagtatt tgcgcgataa cccgacggcg tccgactggt acccgtggtc
     4681 ctggtaccgc gccccgggcg actcgggcaa gtcgtggacc acccccccgc tcgagtttgg
     4741 cgactacgtg ttcgcgattc gcgccaagga cgaggcgggt gcggtgaccc cgacgctggt
     4801 cgagccggtc aacgttcgcc gcatcaaagt ggctccgcgc atctcgggac cgaccctcgt
     4861 ggtcacgagc acactgatcg gcagcgtgat cgccgcctcc tgcgactacc cgctgacgat
     4921 cgtcgacgtg ccggccggcg tggacctcgc gttctcgttg agtgcctgcg cggagagcta
     4981 cggcggaacc gtcgccggct atcgctacgg ctgggacatc ctggacttga acgaccccga
     5041 ccaatgggaa gtcgactaca cgccgttcat cggggcggtg gcgaccacgc ccgcgcgcgc
     5101 attcaacttc ggcgtgcaca cgttcacgtc ggaagtgatc gacaactcgg gcttctgcac
     5161 tcgcatcgag atcaaggtca acatcgtccg gttcacgggc gagcgtaact tgctcgtcat
     5221 cgacgactac aagtccgacg aggtcgtcgg acagtcgggg tgggctgcga ccaatggcgg
     5281 cgttcccaac gactcggagc acgatgcctt ctgggtcgac atggtctcga acctcgacat
     5341 cttcgacccg agccgcgaca tggtcgccac gtcccgcgat agtgagttgc cgctgaccac
     5401 gctcgcgggc tacaagagca tcatcttcag cgtgttcagc gacctcgaca cccgcagccc
     5461 gtccgatctg ccgctcttgt acacgtacat ccagtaccgc tccaagcgtc ctcccgcgag
     5521 cacaccgggg gcgtgcagct catcgggcgg tgtccagggc aaggtccttc ccaacttgat
     5581 cggactcgcc atgcaggcgg gcgtacacgt gctcatcgcc ggtaaccacc ccgctcaaaa
     5641 cgcgctctct cgcaccgcca gtccccccgt gcgctggccc gcgcttccgc tctacgagtt
     5701 cgagcgcggg agtacgcaga ccggaagctc cccgcaggac ctcgataatc cgccgggcga
     5761 ccacgccttc gcctatcgcg acctgtgtct cgacgtcatc gactacggat tcttgaccag
     5821 ctcgcgcgct cgcgtgcgag gcggcggctc ggtcgccaac cagcgttact gccccatcaa
     5881 cgcgtggcgc agtcccaact cccagtcgcg gcgcgacgac ggcatgcgcg cgggtgttcc
     5941 ggccgatccc aacttcccga ggatcgagct gcggccggag gcggccggga ccggacgcct
     6001 gttcaatccc gcgtcgcagg cgatcgacgt cgaggtgtac aacccggctt acttccggca
     6061 gggccaggcg tgcgcgtggg tgcagccacc gcggccgtgc ttcgagccga tctatctctt
     6121 ggagtgcctc gacaccgccg aggcgacgta cttgcagccg atcgcgttct ggaccaccac
     6181 ctacgccgac gtcgtctcgg aggacatccc gggcgcagtc gcggcccgca gcgcggtgtt
     6241 cggcttcccg ccggtgtact tcaatccggc gcaggtgaag cccgccatcg agtacattat
     6301 gttcgacgag tggcagctcc cgcggcggcc ggtcggcacg gcgtcgcagg ggcgccttga
     6361 cgggacggca ggggcgcact aagataccgg cacgttcgac gtggcacggg tcgtgcaccg
     6421 gttcgaaccc gaggttatcc attcgatccg aagactcgct tcgtgacatc ttcccccgtt
     6481 agcggccccg cgcgcacgcg cgaccgggcc gcgcatctcg cgcgcatcct atccgagcgt
     6541 atcctcatcc tcgatggagc gatggggacc atgatccagc gctaccagct ggacgaggcc
     6601 gccttccgcg gcgaccggtt tcgcgaccat cccaaggacc tcaagggcga caacgacctg
     6661 ttgtgcctga cccagcccaa gatcatcggc gagatccacc gcgcgtattt cgacgcgggc
     6721 gccgacgtcg ccaccaccaa caccttcaac ggcacctcga tctcgcagca cgagtacggg
     6781 ctggaggcgt tcgtatacga gatctcgcgc gaagcggccc gcatcgcgcg cgccgaagcc
     6841 gacgccgccg aagggcgcga cccctcgcga ccgcgcttcg tggcgggctc cttggcgccc
     6901 accaaccgca cggcctcgat ctcccccgac gtcaacgacc cgggctttcg caacgtgacg
     6961 ttcgtccagc tccgcgacgc gtaccgggaa aacgcgcgcg gcctgatcga cggcggcgcg
     7021 gacattctcc tggtcgagac cgtgttcgac acgctcaacg cgaaggcggc cctattcgcg
     7081 atcgaagacg aattcgaggc gcgcggatac cgcttgccgg tgatctgctc gggcaccatc
     7141 accgatttat ccgggcgcac gctttcgggg cagacgccgg aggccttcta cgcctcggtg
     7201 tcgcacgtac cgctcctcgc ggtgggactg aactgcgcgc tcggatcagc ccagatgaaa
     7261 cccttcctcg ccgccctcgc gagtgtcgcg cgcgtgcccg tgagcgtgta tcccaacgcc
     7321 ggcctgccca acgccttcgg cggctacgac gagacgcccg atgaaatggc ggccaacctg
     7381 cgcgacatgg ccgacgacgg ctggatcaac atcgccggct cgtgctgcgg gtcgaccccc
     7441 gagcacaccc gcgccatcgc ccaggcgatg cgcggtgtgg ccccccgccg tgcgcccgag
     7501 cgcccgcgct acacgctcct ctccggtttg gagccgctcg agatccggcc cgattcgaac
     7561 ttcatcaatg tcggcgagcg caccaacgtt accgggtcgg cgcgctttcg ctcgctcatc
     7621 aaggagaacg actacgaggc ggccctgacg gtcgcgcgcc agcaggtcga agacggcgcg
     7681 cagctgatcg acgtcaacat ggacgagggc ctgctggact ccgaagccgc gatgcggcgc
     7741 ttcctgaaca tcgtctccac cgagcccgcc atcgcgcgcg tccccatcat gatcgacagc
     7801 tccaagttcg aggtgatcga agccgggctc cagtgcgcgc agggcaagtg cgtggtcaat
     7861 tccatttccc tgaaggaagg cgaggcggcg ttcatcgcgc acgcgcgccg cgtgcgacgc
     7921 tacggtgcgg ccgtgatcgt catggccttc gacgaaaatg gccaggccga tacggtcgag
     7981 cgcaaggtcg agatctgcgc gcgctcgtat cgcatcttga ccgagcaact cgacttcccg
     8041 cccgaggaca tcatcttcga ccccaacgta ttcgccatcg cgaccgggat cgaggagcac
     8101 aacgcgtacg cggtccactt catcgaggcg tgccgcgagc tcaagcagcg ctttcccctc
     8161 tcgcacgtga gcggcggggt gagcaacgtc tccttctcgt tccgcggcaa cgaaccggtg
     8221 cgacaggcca tccacgccgt gttcttgtac cacgccattc gcgccggcat ggacatgggc
     8281 atcgtcaacg cgggccagct gatggtctac gaggacatcc cgaaggatct gctggaacgc
     8341 gtcgaagacg tggtcttgaa tcgccgcccc gacgccaccg agcggttgct cgaggtggcc
     8401 gagtcgatgc ggcgcggcgc cgaccccagg aaaaaggacg acgaagcgtg gcgcgcgctc
     8461 ccggtcatcg agcgcctgaa gcacgcgctc gtgcacggtt tggacgcgca catcgtggaa
     8521 gacgtcgagg aagcccggca agccgcggcg cgccccatcc acgtgatcga aggcccgctc
     8581 atggacggga tgaacgtggt cggcgacttg ttcgggagcg gcaagatgtt cctgccccag
     8641 gtggtcaaga gcgcccgcgt catgaagaag gcggtggcct ggctgacccc cttcatggag
     8701 caggagaagg agaggacggg gcggcgggag gcggaggccc ggatcgtgat ggccacggtc
     8761 aagggcgacg tgcacgacat cgggaagaac atcgtgggcg tggtcctggc ctgcaacaac
     8821 tacgagatcc tggacctcgg ggtgatggtc tccgcggacg ccatcctgaa ggcggcccgc
     8881 gagtcggggg ccgacctcgt cggcctctcc ggcctcatca ccccctccct cgaggagatg
     8941 gcccacgtgg cccgcgagat ggagcgacag gagttcaagc tccccctcct catcgggggg
     9001 gccaccacca gcaaggccca caccgcggtg aagatcgccc ccgcctacag ccagcccgtg
     9061 gtccacgtcc tggacgcctc ccgggcggtc acggtggcga gccagctcaa gagcgccggg
     9121 cagcgcgagg ccttcgccgc gcagaaccgg gccgagcagg acgcgctgcg ccggcagcac
     9181 caggacaaac gctccgcgaa gccgctgctc accctcgcgg aagcccgccg ccgccgtccg
     9241 cgggtcgact gggaatccta cgagatcccg c
//
LOCUS       DASPGK010000413        26289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_919725, whole genome shotgun sequence.
ACCESSION   DASPGK010000413 DASPGK010000000
VERSION     DASPGK010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919725"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VEC56_11320"
     CDS             <1..303
                     /locus_tag="VEC56_11320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14784.1"
                     /translation="NLGVAYYNLRDEDPNALAESVSYYQKALDLQPDDPATVLNIVLS
                     YTAAKDWDQVVQWGEKLVNISPTDPASWRLLAMGYKELGDMDKFQETMARYDMLAK"
     gene            598..1845
                     /gene="rho"
                     /locus_tag="VEC56_11325"
     CDS             598..1845
                     /gene="rho"
                     /locus_tag="VEC56_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXV14785.1"
                     /translation="MDIADLKGKSISELLEVANSLELEGTSSLRKQELIFKILEAQTQ
                     QNGLIFAEGVLQVLPEGYGFLRSPDYNYLPGPDDIYVSPSQIKKFDLRTGDTVSGQIR
                     SPKDSERYFALLRVEAVNHEHPDVAKTKPFFDNLTPLYPQEAFNLEHDPEDLSTRLIN
                     LMCPIGKGQRSLIVAQPRTGKTILLQKIANAITANHPEVYLIVLLIDERPEEVTDMER
                     SVKGEVISSTFDEPAERHVQVSDMVIEKAKRLVEHGKDVVILLDSITRLARAHNAVVP
                     HSGKILSGGVDSNALQRPKRFFGAARNIEGNGSLTIIATALIETGSRMDEVIFEEFKG
                     TGNMELVLDRRLSDKRVFPAVDIFKSGTRKEELLIDKKDLEKIWILRKFLNDKPLMEA
                     MEFFLDKLGKTKTNAQFLKSMNA"
     gene            1930..2997
                     /locus_tag="VEC56_11330"
     CDS             1930..2997
                     /locus_tag="VEC56_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14786.1"
                     /translation="MKRLHGWLIVGLLGMATLAIAKSVETTSGIIYNRNGQFEEARVI
                     LLKALAKDPKDAEAHFQIGYSYSNLDSVALAYRHFVKARELDPKKSKDVANNIASNYA
                     KHYKLGQNAFGRTDFAAAEHEFALATQADPTQSAAHYNLAVTYARLSASDSTYDVKAL
                     TEADQVLALSDASDPNYTKALQLAARLLAQMGREAEAVERFKPMIEKDPSKYPVVEET
                     GMELLKAGNWEGAAAFLKLSADARAKVGAEDFAVYSNVGVAAFNSRKDDPAMLDEAIG
                     YYEKALAIQPDDAPTVLNLMAAYMAKEDWENASTWGEKYVSVSPSDPKGWQYLARCYG
                     ELGDTDKASEALKRYQELKGQ"
     gene            3194..3454
                     /locus_tag="VEC56_11335"
     CDS             3194..3454
                     /locus_tag="VEC56_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014934191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HXV14787.1"
                     /translation="MKKETHPEYHPVVFVDPSVGAEFITRSTMKSAETRDIDGVKHYV
                     VKMEISSASHPFYTGKRKFVDTAGRVERFREKYKYKDTGSSE"
     gene            3466..4368
                     /locus_tag="VEC56_11340"
     CDS             3466..4368
                     /locus_tag="VEC56_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HXV14788.1"
                     /translation="MPEKRISVGGQAVIEGVMMRSPTVYATAVRTPQGTIVIQKNRFV
                     AITHRFKFLNVPIVRGAINLVEMMWIGIQSLSYSASQAAEDSDPKPKKTATTNLSIFG
                     SVVFALGVGFLLFFYLPLWLTELLGIQSSIWFNLVDGAFRLIIFLGYLFAIAQWKEMK
                     RVFQYHGAEHKTIYVFENGQDLTVANARPYTTLHPRCGTSFLLIVMLVSIFVFALLGK
                     PETWGDRLLRFAFIPVIGGIAFEFIRLSAKEKFARFLRPAIWPGLTLQKVTTREPSDD
                     MLEVAIAALVACLDYQNARLEIPS"
     gene            4420..5499
                     /gene="prfA"
                     /locus_tag="VEC56_11345"
     CDS             4420..5499
                     /gene="prfA"
                     /locus_tag="VEC56_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXV14789.1"
                     /translation="MNPKYDQILARFDELTSLLSDEKVIRDNARWRKLAKERSDMTEL
                     VEVTSRLKRVDETVADNEQIIKAGEDAELVEIARAEMDALRAERTTLEEQFQLLIVPK
                     DPADDKNTIMEIRAGTGGEESALFAGDLLRMYTRFIERKGWKAEILSSNETGMGGFKE
                     VIVLVAGTGAFGTLKFESGVHRVQRVPATEASGRIHTSAASVAVLPEAEEVEVHIDPN
                     ELRIDVYRSSGPGGQSVNTTDSAVRITHIPTGLVVTCQEERSQLKNKAKALKVLQARL
                     LDRARSAQEEKMAADRRSQVGSGDRSAKIRTYNFPQQRVTDHRINFTTHNLSEVMEGY
                     LDELIDALRKADIMERAESAARSAE"
     gene            5501..6379
                     /gene="prmC"
                     /locus_tag="VEC56_11350"
     CDS             5501..6379
                     /gene="prmC"
                     /locus_tag="VEC56_11350"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXV14790.1"
                     /translation="MTAPRPENVREWLKHAESHLVSHGVPNARRNAEWMLCHSLGWSM
                     LDVYVQSAAPIDNESARRYWEIVERRASREPLQYILQGTEFMSLPFEVRPGVFVPRPE
                     TEVLVERAEARLRARPLHEPLAVLDLCCGTGIVGVSLAYRVPNLDVVAVDASPEAVDL
                     TAANANRNAVTDRVRPVQADGFDFLEHFEGRFAAVLCNPPYIPTAELAALPREVRDHE
                     PMRALDGGADGLDFYRRVAPLLARRLHANGFVMFEIGDTQAAAVSALLQSAGFAGVGV
                     DQDLAGCDRVVWANKE"
     gene            6381..7634
                     /gene="murA"
                     /locus_tag="VEC56_11355"
     CDS             6381..7634
                     /gene="murA"
                     /locus_tag="VEC56_11355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXV14791.1"
                     /translation="MDKFVIEGRTPLHGEVSVHGAKNAVLPLMAAALLARGKSVIENV
                     PDLRDVATMVKMLDLLGAVTTRTDGDLVIDTTHANGIEAPYDLVRTMRASIYVLAPTL
                     AVHGRARVSMPGGCAWGPRPIDLHLMAMEKLGAKVELSHGYIEATCERLRGGEVVFPI
                     SSVGATAQTIMAASLADGVSVIENAALEPEITDLVDALVECGVEIEGRGTSRVVVHGT
                     TSVKPLKHRVIPDRIEAETFAAAAAITGGDVTIVDCDPAHMGSVFAVLEAGGCKIDAG
                     ERRVRVVGPERLAATDVVTREYPGFPTDMQAQIIAALCTADGVSTVKETIYPDRFTHV
                     PELRRFGADIRLDGNLAVIRGVPSLSGAPVMATDIRASSGLILAAMAAEGRSEILRVY
                     HIDRGYQRIESKLNALGARIERVVQ"
     gene            7636..9567
                     /gene="selB"
                     /locus_tag="VEC56_11360"
     CDS             7636..9567
                     /gene="selB"
                     /locus_tag="VEC56_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HXV14792.1"
                     /translation="MASSDPRSPRHFILGTAGHVDHGKTELVKALTGHDTDRLKEEKE
                     RGISIELGFAPLPLAPDVFVGVVDVPGHERFVRQMVAGAGGIDLAMLLVAADEGVMPQ
                     TEEHVEVLRSLGVESGLVVISKSDLATADTLAIVREETATLVRGTFLEKAPVVPVSAR
                     TRDGLDALRENLLTLTRALVGRVSSGPFRLAIDRVFHQKGIGVVVTGSCYSGRVRVGD
                     ELALLPAARSVRVRDLESFGEKREEGAAGERLAIALHGTKLDEVARGDMLATPGRFGV
                     TRLLDARVAMGAFAGRPLKNRERVRIHHGAREVLGRVVLLEVDELAAGATSLAQLRLE
                     APLVASAGDRVVLRSYSPARVVGGGVVVDAAPEVHRRSDTAALERVRHQERGEPASVL
                     AQAVARAGANGIAESDANADLATALADRGVLVAIAGRWFHRDALDDLAARAIEIASKH
                     TSRHALQWGVDKEELRRRLSFPHGPAVFHRVLEELASSHALFVREDRVRAGSPEHAIA
                     PALARSLSSLNDTVRAAGVAFPTREESERAWSGTEPFAEAARWLRDAGEWVDVGTGWM
                     HREALARSIEAARLLLSRQPAISVADFKDALGITRKHAIPLLEYFDRQRLTVRRGDLR
                     LPGPGLASPRSPHLPPENL"
     gene            9624..10013
                     /locus_tag="VEC56_11365"
     CDS             9624..10013
                     /locus_tag="VEC56_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14793.1"
                     /translation="MKRLLPRMAVLALGLALVLGAVSSFGQSLPLPLEKKIMAGQTTI
                     EYVGQSLRFTTTVPLYVKLEPVSLTQIRFTVRVHPGYAIPNGPVGSAENNLEIYWVNV
                     GTDVYEGGAPSGTIEGVLDTEGGFVDR"
     gene            complement(10479..11936)
                     /locus_tag="VEC56_11370"
     CDS             complement(10479..11936)
                     /locus_tag="VEC56_11370"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HXV14794.1"
                     /translation="MNASLLAKLVREIRDDVVGRPLGTPSWFAPVLAIPVYGRGHLVA
                     VLESPGPFCYVVGDSPFEGAQAPARMRMLSGATITDVRLGEARVLRVDAETRDGDHVS
                     LLITLFGSAGSASLVRGTSTVETLGKGRVPSASAPPTAVAVLETPFRLVVHGNLGGAT
                     PAHDVTNAEEDVHVLGPFDSARAACASLGARVLEGAHATILLRITRPLRRRVDALAKL
                     SVKLEEDIARAAQHGELRREAETLAAFQTRVPAGAASIELPDVYDASRIRTIAIDPAE
                     SLHLQIEKRFRRAAKLERSAEHAVRRLELVRREHQDLGASLAVLEGSRSFNEALKTIE
                     AIRAKFDIAIDDAPRPQGAPKKREAEKTYRTFDVDPRWFVLVGRSNFENDEITFQVAK
                     PTDFWFHAQGVPGSHVVLRSRGGSDGPPARVIERTASIAAYFSKAKHSSLVPVIYTQR
                     KYVRKFRGARPGQVTCEREKMVVVPPVIPEGADSA"
     gene            12008..12883
                     /locus_tag="VEC56_11375"
     CDS             12008..12883
                     /locus_tag="VEC56_11375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXV14795.1"
                     /translation="MLISMTGFGQGEAANAHSTVTVEVRSVNHRFLDIAFKLPRSLQN
                     REPDIKEIVRGKIARGRVSITISVESASTGRGVVINEIVLEQYLAQLRAFAKRHDLDE
                     RISLDALVTLPEVVTTKETEPGEDELWPLVDKSLGEALDACNRMRFDEGKALEKDLVD
                     RMRLIDKTVAAIEKLAPAVAKRQVETLRKRVAQLAGEVAVPEDRIAAEIVMLSDRTDI
                     TEEVTRLRSHIAQFNGALKDGGEVSKRLTYLLQEMHREASTMGSKASDTEVIQHVVVL
                     KEETEKLREQVQNLE"
     gene            12880..13509
                     /gene="gmk"
                     /locus_tag="VEC56_11380"
     CDS             12880..13509
                     /gene="gmk"
                     /locus_tag="VEC56_11380"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXV14796.1"
                     /translation="MILARASSAFLIVLSGPSGVGKTTLVDRLLADDPLVRRSISATT
                     RTHRAGEVDGRDYFFVNRERFEAMKAGDLVEWADVHGALYGTPRRFLEGELAAGRDVI
                     LNIDIQGGDQVKRSFPGAVMVFILPPSFRVLEDRLRARGDVSGDGLKVRLANARKEIE
                     AGARYDYIIVNDSVERAAAELAAIVTAERCRRQRMAPGFIADLSNQQAK"
     gene            13524..13772
                     /gene="rpoZ"
                     /locus_tag="VEC56_11385"
     CDS             13524..13772
                     /gene="rpoZ"
                     /locus_tag="VEC56_11385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXV14797.1"
                     /translation="MSIPIEKLVIIEKITQKIQNRYEAVRVMAREARRLNSLVIRGAE
                     PEPDFKPTSTAVKRLINDKIQFSYADEPTGSGDFFQES"
     gene            13782..14978
                     /gene="coaBC"
                     /locus_tag="VEC56_11390"
     CDS             13782..14978
                     /gene="coaBC"
                     /locus_tag="VEC56_11390"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXV14798.1"
                     /translation="MSLSGKKILLGVTGGISAYKSVFLLRLFKKAGADVKVVMTEAAA
                     EFVTPLTFESLSEHPVHIRMFEGEGRSAGAISPVEHIDLAKWPDLVVVAPATANTLAN
                     FVHGRADDLLATIVSAYQGPIMLAPAMNDVMWENAATQENMRILSDRGFRVAPPEKGD
                     LACGYEATGRMAEPETIFAAAQTLFSSPLRGKKVLVSVGGTEEDIDPVRVISNRSSGK
                     MGFAVAEAARDAGADVTVVAARTSVAAPHGLRIVRVRTGDEMAKALKAEFKNADALFM
                     VAAVSDFKPASTMAQKKKSGEGWTLELSKTDDILASLGADKGKRVVVGFALETEDEEQ
                     NARAKLAKKHCDFVVLNKPGDAFGLDTNVVSVFDATGAVYQSKAPESKREIARKLLDL
                     IAGSKR"
     gene            complement(15008..15886)
                     /locus_tag="VEC56_11395"
     CDS             complement(15008..15886)
                     /locus_tag="VEC56_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14799.1"
                     /translation="MRILVRTLVYCVLVTAASQASAQYGWLQNDDFEEGQSVGCSFAV
                     IDEKAFAALFTAAPTWYPYEIERIRILHCGAGDGIITFTIWQDAGNLDPGTLLFLSSD
                     TYLFSGTPGGAQFIDIVIPPGTVPMITSGTIRVGIDVLFSSPGFAYDTSGMTAQRNFF
                     RNGTTWSYTENPPLNLTGDWILRLGVVTPPTAVDDEQMPSGFALVGNFPNPFNPTTTI
                     RYDVPVRGGHVNIAVFDVTGRHVQTLVDDHREAGRFDVQWDGRGARGERLGSGVYFLR
                     MRAGTFTEAKKIVLLE"
     gene            16111..16710
                     /locus_tag="VEC56_11400"
     CDS             16111..16710
                     /locus_tag="VEC56_11400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXV14800.1"
                     /translation="MTENRTTSTRVATPALPPGLSGLAKTVSVCTRCDLHKSRTQTVF
                     GVGNPNAKLMFIGEAPGAEEDARGEPFVGRAGQLLTKIIAAMGMTRDEVYIANILKCR
                     PPQNRDPLPSEVECCEEYLRAQIAMIKPKYICALGRIAAHWLLKTEAPLGALRTGSYT
                     YEGIPVIVTYHPAALLRNPAFKAPCWEDMKKLMALLKDA"
     gene            16756..19089
                     /locus_tag="VEC56_11405"
     CDS             16756..19089
                     /locus_tag="VEC56_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HXV14801.1"
                     /translation="MIGQTISHYRIQERLGQGGMGEVFLAEDLRLHRSVALKILRQRL
                     GCEDEERARIMREARAASALNHPNIAVIYDVEETETPDGRVYLLSMEYVRGKTLADLA
                     AGSSLSLDDILDIVAQTADALAEAHARGVVHRDVKPSNLMVAQGRVKVLDFGLAQMQP
                     RLRADDLTWSRDATHAPEGVFAGTPHYMSPEQALGRTLDSRSDIFSLGVVFYELLAGK
                     RPFEGDTFVQIADAILHRDPPPLPVRFSDIRAPEVERLMTRMLAKDAKARPRDLREIV
                     VELQRLRAGASPLTAQSGELTVAVAGFAGIGERAEDEWLGTGLSETVTTALQEVEGLV
                     VWGREQLRESLRRLGVEAAELAPEDAVQLGRMIGARWVLSGAYQRLGEQVRVTARVVE
                     VESGRIIRAVKQDGRLDAIFDLQDRIVTELASGLRGSVAAAHEGEETKIVAAYEALAK
                     GLLNVRADSYESLDRAILFFERAVALDPDYVRAQIELAAALEQKGNYLTAPELHERAA
                     AILRKVLEVRPRLARAWRELGMSLISLGRVRDGVENLRRALSLAPDDARTQAGLARGL
                     FIGMADFRAAAEMFERSVERNPEAGWYWMQLAHCCAFLREFKRGEKAAKRAIELQEAF
                     LSGQQGVQLVGAYMRLGHLLALQGRPRDAHDANASESLFLEKIDHALRSRIRIELQMR
                     LGASLIDLGDQRGARAAFEVGLDAFTQRLALGADDPFTRYYAAAIHALLGQDDEAIAL
                     LERAVTAGTRAFVIARARIEPEWNGLRSNPRFARLTA"
     gene            19104..20210
                     /locus_tag="VEC56_11410"
     CDS             19104..20210
                     /locus_tag="VEC56_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXV14802.1"
                     /translation="MPSTDPNLPVEGWLDIGAAAELSKRAVQQVLLGTTKVALTCKDG
                     EFGAISGNCNHAGGPLGEGTMEGDYVVCPWHQWRFHRSQGVGEPGFEEDAVPAYHVRV
                     REGRVEIHPKPYTKRSKKPHDPHPLSREIRREDGPIRVVGISTTVMNQDYPRYSTSDA
                     LLEEAIRHADATSHETKLIKLRELSFRSCEGYYSKSAHACTWPCSITQMDPNDQLDRV
                     YEAFVHWGDVFVIATPIRWGNASHLYYKMVERMNCIQNQMTIANRTLLRDKVVCAIVT
                     GGQDNVQGVVGQVLGFFAELGCHFPQYPFIAHSRGWSAEDMEANVIAVQHSEELRAGA
                     RGLVDRGIELAERLLATTPHKAERGGRKGKALRD"
     gene            20338..21702
                     /gene="dnaB"
                     /locus_tag="VEC56_11415"
     CDS             20338..21702
                     /gene="dnaB"
                     /locus_tag="VEC56_11415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXV14803.1"
                     /translation="MAEAIRTAQTQLQAPGRVPPQSLEAEMAVLGAVLLDNNAFSIAT
                     ESLSSDAFYKKAHKDIFAAMEGLSARGEAIDVVTLSEELKQRGTFQSSGSATYLTQIM
                     DSVHTAANVEYYANIVLEKFVMRRLITISNDIATQCYSGEREASELLDEAEKHIFEIS
                     QQGMFKGFEPIGKILRDHFKNIEALYQSGSHISGTPSPFEELDSLTSGFQKSDLIIVA
                     GRPGMGKTSFALNLCQHLAIKEKTPVGIFSLEMSAEQLVTRLLCSEARIDSNRLRRGY
                     LKSNEYAELAIVAGYLAEAPIYIDDSAGLSTLELRAKARRLKAEANIGLLMVDYLQLI
                     SVRERVENRQQQISLISRGLKALAKELNIPVIALSQLSRAVESRGGDKRPMLSDLRES
                     GSIEQDSDLVLFLYRPEVYDTSDENRGKAELIIGKQRNGPTGTVNLTFIDSCTRFEPA
                     AFTE"
     gene            21710..22525
                     /locus_tag="VEC56_11420"
     CDS             21710..22525
                     /locus_tag="VEC56_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14804.1"
                     /translation="MSAPRTTITQALRDLGATILNFLRDVGGSSIMLAGVFARLRELP
                     RTAEQTLLQMQLIGIGSIPLVLVTSVFVGAVAAVQAAYQFQDYVPMVFLGTVVGKSVI
                     LELGPVLTALVVGGRVSASIAAELGTMKVTEQIDAMEVLAIDPLRYLVMPRVVACVVM
                     LPVLTIFADFLAILGGMFVANATVGVDYATFVEGLRLFFYVDDVISGVIKAATFGGII
                     GLMGCYNGFRTFGGAQGVGRATMQAVVSSCICILITNYFLASVLLRVIFYKGD"
     gene            22597..25017
                     /locus_tag="VEC56_11425"
     CDS             22597..25017
                     /locus_tag="VEC56_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV14805.1"
                     /translation="MLRPRWTTLALVTTTLALGLHASLAHAAWQQNGNRVSDVSLGQA
                     RPAIAPDGEGGAIVAWVENRSGLFLDIYAQRLDSWGRPVWATGGVAIAVVAWDQDNPI
                     VVSDGEGGAIIAWEDSRNGTDSDIFAQRVSTAGTPMWPPNGVAVCEQPFHQNKLRMTR
                     SDLNGVIIVWQDVRANPDWNIYGQRLDGLGIAQWPANGIAVCAAVGNQFDPDIVSDGF
                     GGAIVAWEHQVGFDYDIRAQRVHVSGGVMWSLDGESLCSNLATQGDVKLVSDDAGGAI
                     AVWSDSRQSPAALFAQRIVQYGYTWWLSDGVEVTTTTGDYETDYQVVADGSGGVIVVY
                     NRAFALIMGQRINPFGSRMWTASGMTVSPALIQEMSPHVASDGLGGAIVSWIDYRNVV
                     PDVFARRVDATGVPVGPAGGTPIAAVPGGQMPAPMVSDGAGGAIMAWQDYRAIDYPHV
                     YAQRMDRLGNWGYPAGDIVSALDVPGDQGGVINLQWTASRLDPWPNQEPDRYTVWRAI
                     DPTLASVFASSGTALVSPGELDPSATEALRVETIAGQTYYWQLVSTVDAFYLNSYAQA
                     VPTLFDNTISSPGPHYFQVIAHGFTPQEYWVSAPASGDSFDNLAPAAPIMLAAQRVGN
                     DVELTWNPSGANELDFAEYAVYRASSAGVQPIPGFFVTGTPDTVLVDSSPPPGYLYYI
                     VTARDVHDNQSAPSNEASVTIPTGIGDDAPALTRLTLDGNFPNPFSRDTELRIGLPRG
                     SDVRLEVFDVAGRRVATRDVPRLSAGWQRVDFDGRDARGNPLASGVYFGRITAAGETQ
                     TMKMVIRR"
     gene            25102..25863
                     /locus_tag="VEC56_11430"
     CDS             25102..25863
                     /locus_tag="VEC56_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14806.1"
                     /translation="MVTIECKDVWKSFGRKSVLRGLNLTIQPGETVVIIGQSGSGKSV
                     LLKHIVGLIYPDKGAILIDGIDITKLNRKKLFELRMGFGMVFQGAALFDSYTVEENVG
                     LAMREHTELSDEEVNRIAEEKLRMVGLDGIGDKHPAELSGGMKKRVGFARAIAMEPKC
                     VLYDEPTTGLDPINADVINNLIVKLSNELHITSIVVTHDLASAYKVGDRIVMLHDGKV
                     LFDGTPEQVKMTTNAIVRQFIEGRAEGPIVVGSGE"
     gene            25860..>26289
                     /locus_tag="VEC56_11435"
     CDS             25860..>26289
                     /locus_tag="VEC56_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXV14807.1"
                     /translation="MTSRGLEIRVGAVVIAAALVAVIGTMWFQKFQLAEKRYPFYVRF
                     DEVGGLVSGDPIQVNGVERGRVDRVDLLPRGVVVQMAVREGVVIPLDSQIALKSIGIM
                     GERFVAIVQGDSSVAIAPGDTVDGSYLMGLSEVMGAAGGIL"
BASE COUNT         4794 a         8521 c         8555 g         4419 t
ORIGIN      
        1 aatcttgggg tcgcgtacta caatctgcgc gacgaggacc cgaacgcgct cgcggagtcc
       61 gtgagctact accagaaagc gctcgatctc cagcccgacg accccgcgac cgtactcaac
      121 atcgtgctct catatacggc ggccaaagac tgggaccagg tcgtgcaatg gggcgaaaag
      181 ctggtcaaca tcagtcccac cgatccggcg agctggcgct tgctcgcgat gggctacaaa
      241 gaattgggcg acatggacaa gttccaggag acgatggctc gatacgacat gctcgccaag
      301 tagggccgaa gcccgtcggg cgacgccccc cgcgcgccgg agtggaacgc gaattgcagc
      361 gtttcgcgga tggcggggtg gggtggcggg caccggggcg atcaccgaga ctcgtaggtt
      421 ctttctctca aaccgattag cactcattcc agcaaccatc gatcgacgcc ctagacggcg
      481 actaagttct caatcaatgt gacggttcgt aaaccaccgt taacacgtta aaacctctct
      541 cccatcgggc ggccaagccc gaacctacct atcgtgtctt cattaggagc aaagcgaatg
      601 gatattgcgg acctcaaggg gaagagcatc tccgagctgc tcgaggttgc caattctctc
      661 gaactcgagg gaaccagcag cctgcgcaag caagagctga tcttcaagat cctcgaggcc
      721 cagactcagc agaacggtct cattttcgcc gagggcgtcc tgcaagttct ccccgagggc
      781 tacgggttcc tccgttcccc cgactacaac tacctgccgg gcccggacga catttacgtc
      841 tcgccgtcgc agatcaagaa gttcgacctc cgcaccggcg acaccgtctc cgggcagatc
      901 cgcagtccca aggactcgga gcggtacttc gcgctcttgc gcgtcgaagc ggtcaaccac
      961 gaacacccgg acgtcgccaa gacgaagccg ttcttcgaca acttgacgcc gctctacccg
     1021 caggaggcct tcaacctcga gcacgatccc gaggatctct cgacgcggct gatcaatctg
     1081 atgtgcccga tcggcaaggg gcagcgaagc ctgatcgtcg cgcagccgcg taccggcaag
     1141 acgatcctct tgcagaagat cgccaacgcg atcaccgcca atcaccccga ggtttacctg
     1201 atcgttctct tgatcgatga gcgcccggaa gaagtgaccg acatggagcg ctccgtcaaa
     1261 ggagaggtca tttcctccac cttcgacgaa ccggccgaac gccacgtcca ggtctccgac
     1321 atggtgatcg agaaggcgaa gcgcctggtg gagcacggca aggatgtcgt aatcttgctc
     1381 gactcgatca cgcgactggc gcgcgcgcac aacgcggtcg tgccccactc ggggaagatc
     1441 ctctccggcg gcgtggactc gaacgcgctc cagcgcccga agcgcttctt cggcgcggcg
     1501 cgcaacatcg agggcaacgg gagcctcacg atcatcgcca ccgcgttgat cgagaccggc
     1561 agccgcatgg acgaagtgat cttcgaagag ttcaagggca ccggcaacat ggagctcgtg
     1621 ctggatcgcc gcttgagcga caagcgggtc ttccccgcgg tcgacatttt caagtcgggc
     1681 acgcgcaaag aagaactttt gatcgacaag aaggacctcg agaagatatg gatcttgcga
     1741 aaattcttga acgacaagcc gctcatggag gcgatggagt tcttcctcga caagctcggc
     1801 aagacgaaga caaacgcgca gttcttgaag agcatgaacg cttaggatct acggcgtcta
     1861 cgtcgtagtg cagggaagag gccccacccg cccgggttgg gcctcttcgt tctttacgga
     1921 ggtaaggcag tgaaacggtt acacgggtgg ttgatcgtcg ggttactcgg aatggcgacg
     1981 ctggcgatcg ccaagtcggt cgaaacgacc agcgggatca tctacaaccg caacggccag
     2041 ttcgaagagg cgcgcgtcat tctcttgaag gcgttggcca aggatcccaa ggacgccgaa
     2101 gcgcatttcc aaatcggcta ttcgtacagc aacctcgaca gcgttgcgct cgcctatcgc
     2161 cacttcgtca aggcgcgcga gctcgatccc aagaaatcga aggacgtcgc gaacaacatc
     2221 gcgagcaact acgccaagca ctacaagctg ggccagaacg ccttcggccg gaccgatttc
     2281 gcggccgcgg agcacgagtt cgcgctggcg acgcaggccg accccacgca gtcggcggcg
     2341 cactacaacc tcgccgtcac gtatgcgcgt ctgtccgcct cggactccac ctacgacgtg
     2401 aaagcgctca ccgaggcgga ccaggtgctc gcgctttcgg acgcgagcga ccccaactac
     2461 accaaggcgc tgcagctcgc ggcccgcttg ctcgcccaga tggggcgcga agcggaggcg
     2521 gtcgagcgct tcaagcccat gatcgaaaag gatccgtcga agtatccggt ggtcgaggag
     2581 acgggaatgg agctgctcaa ggccgggaac tgggagggtg ccgcggcgtt cctgaagctc
     2641 tccgccgacg cgcgcgcgaa agttggcgcc gaggatttcg cggtctactc gaacgtcggg
     2701 gtggcggcgt tcaactcgcg caaggacgac cccgcgatgc tcgacgaggc catcggctac
     2761 tacgagaaag cgctcgcgat acagcccgac gacgcgccga cggttctcaa tctcatggcc
     2821 gcctacatgg ccaaggagga ctgggagaac gcgtcgacgt ggggggaaaa gtacgtcagc
     2881 gtcagcccga gcgaccccaa gggttggcag tatctggcgc gttgctacgg cgaattgggc
     2941 gacaccgaca aggcctccga agccctgaag cgttaccagg agctgaaggg ccagtagcga
     3001 gtgccgtcta gtactccagc tcgaggtggc gggcgcggtt cggctgcgca aggtggttct
     3061 ggttaggtag ttacgccgaa agagcggccc ggcgggtccc ggaggacgtg aaggaggggc
     3121 ccaacagccc cttgccccgg cgggccgatt cggttattct tccggctcca gatttcagga
     3181 gtcgcataga aaaatgaaaa aagaaaccca ccccgagtac catccggtcg tcttcgtcga
     3241 ccccagcgtc ggcgccgagt tcatcacccg ctcgaccatg aagagtgcgg aaacccgcga
     3301 catcgacggg gtcaagcact acgtggtcaa gatggagatc tcgtcggcgt cgcacccgtt
     3361 ctatacgggc aagcgcaagt tcgtcgacac agccggccgc gtggaacgct tccgcgagaa
     3421 gtacaagtac aaagacaccg gttcgtccga gtagcccgct cctccatgcc ggaaaagcgc
     3481 atcagcgtcg gtggccaagc ggtcatcgaa ggcgtcatga tgcggtcgcc gacggtgtac
     3541 gcgacggccg ttcgcacgcc gcaggggacc atcgtcattc agaagaaccg gttcgtggct
     3601 atcacgcacc ggttcaagtt tctgaacgtc cccatcgtgc gcggcgcgat caacctggtc
     3661 gagatgatgt ggatcggcat ccagtcgctc tcgtactccg cctcgcaagc cgccgaagac
     3721 agcgacccca agccgaagaa aaccgccacc accaacctgt cgatcttcgg ctcggtggtg
     3781 ttcgccctgg gcgtgggatt cctgctgttc ttctatctcc cgctctggct caccgagctc
     3841 ttggggatcc agagcagcat atggttcaac ctggtcgacg gcgcctttcg attgatcatc
     3901 ttcctgggct acctcttcgc catcgcgcag tggaaggaga tgaagcgcgt cttccagtac
     3961 cacggggccg agcacaagac catctacgtg ttcgagaacg gccaggacct gacggtcgcc
     4021 aacgcgcgac cctacacgac gctccacccg cgctgcggca cgagcttcct gttgatcgtg
     4081 atgctggtca gcatcttcgt cttcgccctg ctcggaaagc cggagacttg gggcgaccgc
     4141 ctgctgcgct tcgcgttcat cccggtcatc ggcggcatcg cgttcgagtt catccgcctg
     4201 tcggcgaagg agaagttcgc ccgtttcctg cgtcccgcca tctggccggg cctgacgctc
     4261 caaaaggtca cgacacgcga accgagcgat gatatgctgg aggtcgcgat cgcggcgttg
     4321 gtggcgtgcc tcgactatca gaacgcgcgg ctggagattc cctcctagcg catcgtcccc
     4381 tcgctcgacg cgcgccgcgc gtcgtcgcgc catcgaatca tgaatcccaa atacgaccag
     4441 attctcgccc gcttcgacga gctcacgtcg ctgctctccg acgagaaggt gatccgcgac
     4501 aatgcccgct ggcggaagct cgccaaggag cgctcggaca tgaccgagct ggtcgaggtc
     4561 acgtcgcgcc tgaagcgggt cgacgagacc gtcgccgaca acgagcagat catcaaggcg
     4621 ggagaagacg ccgagctggt cgagatcgcg cgcgcggaga tggacgcgct gcgcgcggag
     4681 cgcacgaccc tggaggaaca gttccaactc ctcatcgtgc ccaaggaccc cgccgacgac
     4741 aagaacacca tcatggaaat tcgcgccggc accggcggag aagagtccgc gttgttcgcc
     4801 ggcgatctcc tgcgcatgta cacgcgcttc atcgagcgca agggctggaa agcggagatc
     4861 ttgagcagca acgaaaccgg catgggcggc ttcaaggaag tgatcgtgct ggtcgcgggc
     4921 acgggcgcgt tcgggacctt gaagttcgag agcggcgtcc atcgcgtcca gcgggttccc
     4981 gccaccgagg cgagcgggcg catccacacc tcggcggcgt cggtggcggt gttgccggaa
     5041 gccgaggagg tcgaagtgca catcgatccc aacgagctcc gcatcgacgt gtaccgctcc
     5101 agcggtcccg gcggacagtc ggtcaacacc accgacagtg cggtgcgcat cacccacatc
     5161 ccgaccgggc tggtagtgac gtgccaggaa gaacgctcgc aactcaagaa caaggccaag
     5221 gcgctcaagg tgctgcaggc gcgactgctg gatcgcgcgc gcagcgccca agaagagaag
     5281 atggccgccg accgacgcag ccaggtgggt tccggcgacc gcagcgccaa gattcgcacc
     5341 tacaactttc cccagcagcg cgtcaccgat caccgcatca atttcacgac ccacaacttg
     5401 agcgaagtga tggaaggcta tctcgacgag ctgatcgacg cgcttcgcaa ggccgacatc
     5461 atggagcgcg ccgagagcgc ggcccgctcc gcggaatgac atgacggcgc cgcgcccgga
     5521 aaacgttcgt gagtggctca agcacgccga gtcgcacctg gtgagccacg gcgttcccaa
     5581 cgcgcggcgc aacgcggaat ggatgctgtg ccactcgctg ggatggagca tgctcgacgt
     5641 ctacgtgcag tcggccgcac cgatcgataa cgagagcgcg cgccgatact gggagatcgt
     5701 cgaacggcgc gcgagccgcg agccgctcca gtacatcctg caggggaccg agttcatgtc
     5761 gctgcccttc gaagtccggc ccggcgtgtt cgtaccgcgc ccggagaccg aggtgttggt
     5821 cgagcgcgcg gaggcgcgcc tgcgcgcgcg cccgctccac gagcccctcg cggtgctcga
     5881 cctgtgttgc ggaaccggca tcgtgggcgt gtcgctcgcg taccgcgtgc ccaatttgga
     5941 cgtcgtcgcc gtggacgcct cgccggaggc ggtggacctg acggccgcga acgcgaaccg
     6001 aaacgcggtc actgaccgcg ttcgcccggt gcaggccgac gggttcgact tcctcgagca
     6061 cttcgagggc cgttttgcag ccgtcctctg caatccgccc tatatcccga ccgccgagct
     6121 ggccgcgctg ccgcgcgaag tgcgcgacca cgagccgatg cgggcgctgg acggcggcgc
     6181 ggacggcctc gatttctatc gaagggtggc accgctcctg gcgcgccgct tgcacgcgaa
     6241 cggattcgtc atgttcgaga tcggggacac ccaggccgcg gccgtgtccg cgctgctgca
     6301 aagcgcgggg ttcgctggtg tcggcgtgga ccaggacctc gcgggctgtg accgggtggt
     6361 ctgggccaac aaggagtaag atggacaagt tcgtcatcga aggtcggacg ccgctgcacg
     6421 gcgaggtctc cgtgcacggg gccaagaacg cggtgctccc gctcatggcg gccgcgttgc
     6481 tcgcgcgcgg caagagcgtc atcgagaacg ttcccgacct gcgcgacgtc gccaccatgg
     6541 tcaagatgct cgatttgctc ggcgcggtga cgacgcgcac cgacggtgac ctggtgatcg
     6601 acaccaccca cgccaacggc atcgaagccc cctacgacct cgtgcgcacc atgcgcgcgt
     6661 ccatctacgt gctggcaccc accctcgcgg tgcacgggcg cgcgcgcgtg agcatgccgg
     6721 gcgggtgcgc gtggggaccg cgccccatcg acctccatct catggcgatg gagaagctcg
     6781 gcgccaaggt ggagttgagc cacggctaca tcgaagcgac ctgcgagcgc ctgcgcggcg
     6841 gcgaggtcgt ttttccgatc tcgagcgtgg gcgccacggc gcagaccatc atggccgcct
     6901 cactcgccga cggcgtctcc gtgatcgaga acgccgcgct ggagccggaa atcaccgacc
     6961 tcgtggatgc gctggtcgag tgcggcgtcg agatcgaagg acgtgggacc tcgcgcgtcg
     7021 tcgtgcacgg cacgacctcg gtcaagccgc tcaaacaccg cgtgattccg gaccgcatcg
     7081 aagcggagac cttcgcggcc gcggcggcca tcacgggtgg cgacgtcacc atcgtggatt
     7141 gcgaccccgc ccacatgggc tccgtcttcg ccgtcttgga agcgggcggc tgcaagatcg
     7201 acgcgggtga gcgtcgcgtc cgcgtcgtcg ggccggagcg cctggccgcg accgacgtcg
     7261 tcacgcgcga gtaccccggg tttccgaccg acatgcaggc gcagatcatc gccgcgttgt
     7321 gcaccgccga cggtgtcagc acggtgaagg agacgatcta tcccgatcgc ttcacccacg
     7381 tccccgagtt gcgccgcttt ggcgccgaca tccgcctcga cggcaatctc gcggtgattc
     7441 gcggcgtacc gtcgctcagc ggcgcacccg tcatggccac cgacattcgc gcgagctcgg
     7501 gtttgattct cgcggccatg gcggccgagg gtcgctccga gatcctgcgt gtctatcaca
     7561 tcgatcgcgg ctaccagcgc atcgagtcga agctcaatgc gctcggcgcg cgcatcgaac
     7621 gcgtcgtcca gtagcgtggc ttcgtccgac ccgcgttcgc cccgccattt catcctcggc
     7681 accgccggtc acgtcgacca cggcaagacc gaactggtca aagccctcac cggccacgac
     7741 accgatcgac tcaaggaaga aaaggaacgc ggcatctcga tcgagctcgg cttcgcgccg
     7801 ctgccgctcg cgcccgacgt gttcgtgggg gtcgtcgacg tccccggaca cgagcgcttc
     7861 gtcaggcaaa tggtggcggg cgcgggcggg atcgacctcg cgatgctgct agtggccgcc
     7921 gacgagggcg tcatgccgca gaccgaagag cacgtggagg tcctgcgctc gctcggcgtc
     7981 gaaagcggcc tcgtggtcat ctccaaatcg gacctcgcga cggccgacac gttggccatc
     8041 gtgcgagaag aaactgcgac tctcgttcgc gggacatttc tggaaaaagc gccggtggtg
     8101 ccggtctccg cacgtacccg cgatgggctg gacgcacttc gagagaatct cctgacgttg
     8161 actcgcgccc ttgtcgggcg cgtgtcatcg gggccgtttc ggctggcgat cgatcgcgta
     8221 tttcaccaaa agggaatcgg ggtcgtcgtc accggcagct gctactccgg ccgcgtgcgc
     8281 gtgggcgacg agctcgcgtt gttgccggcg gcgcgctcgg tgcgcgtgcg cgacctcgag
     8341 agcttcggcg agaagcgcga agaaggcgcg gccggcgagc gtctcgcgat cgcattgcac
     8401 ggtaccaagc tggacgaggt tgcgcgtggc gacatgctgg ccacccccgg acgcttcggg
     8461 gtgacgcgtc tcctcgatgc gcgcgtcgcg atgggcgcct tcgccggccg cccgctcaag
     8521 aatcgcgagc gcgtgcgcat ccaccacggg gcccgcgaag tgctggggcg cgtggtgcta
     8581 ctcgaagtcg acgagctcgc ggccggcgcg acgtcactcg cgcagctgcg cctggaggcg
     8641 ccgttggttg cgagcgcggg cgaccgcgtc gtcctgcgct cgtactcgcc ggcgcgcgtg
     8701 gtcggcggcg gcgtcgtcgt ggatgcggca cccgaggttc atcgccgtag cgacacggcg
     8761 gcactggagc gcgtccggca ccaggagcgc ggcgaaccgg cttcggtctt ggcgcaggcg
     8821 gtcgcgcgcg cgggcgcaaa cgggatcgcg gagagcgacg cgaacgcgga tctggcgacg
     8881 gcactggcgg atcgtggcgt gctcgtggcc atcgccgggc gctggttcca ccgagacgcc
     8941 ctcgacgatc tcgcggcgcg cgcgatcgaa atcgcgtcca agcacacgtc gcgccatgcg
     9001 ctgcagtggg gcgtcgacaa ggaagagctc cgtcggcgtc tctcgttccc acacggtccg
     9061 gccgtgttcc accgcgtatt ggaagagctg gcgtcgtcgc acgcactctt cgtgcgcgag
     9121 gaccgggtgc gggcgggctc gccggagcac gcgatcgcgc ccgcattggc acgttcgctg
     9181 tcgtcgctca acgacacggt tcgcgcggcc ggagtggcct tcccgacgcg ggaggagtcc
     9241 gagcgcgcct ggtcgggcac cgaacccttc gccgaggccg cacgctggct gcgcgatgcg
     9301 ggggagtggg tcgacgtcgg aaccggctgg atgcaccggg aggctctggc gcgctcgatc
     9361 gaagccgccc gcttgttatt atcgcgccag ccggcgatct cggtcgcgga cttcaaggac
     9421 gcgctcggga tcacccgcaa gcacgcgatc ccgctgctgg agtatttcga ccgccaacga
     9481 ttgacggttc gccgaggcga ccttcgactt ccgggaccgg gcttggcgtc gccccgatct
     9541 ccccacttac cccccgaaaa tctttgagcc cgtctccagt ttttggcttc ccgaatgggc
     9601 gaaaccctgg tatagttact cccgtgaaac gtctcctccc ccgcatggcc gttctcgctc
     9661 tcggcctcgc tctcgtgttg ggcgcggtgt cgtcgtttgg tcagagcctg cccctcccgc
     9721 tcgagaagaa gatcatggcg gggcagacga cgatcgagta tgtcggccag agcctccgct
     9781 tcacgaccac ggttccgctg tacgtcaagc tggagccggt ctccctcacg cagattcggt
     9841 tcacggttcg ggttcacccg ggctacgcga tccccaacgg accggtcggc agcgccgaga
     9901 acaacctgga gatctactgg gttaacgtcg ggaccgacgt ttacgaaggc ggagcgccga
     9961 gcggaaccat cgagggcgtc ctcgacaccg agggcggatt cgtcgatcga tagtcgtctt
    10021 tccgttagtt tttttatctc cccgtaagtt tttttaacca ccgctcgagt ctctcgaccc
    10081 gaaacggccg ttgcttattg tctaactcgt tgctgctacg cggactaggt cccagcgacg
    10141 ataggcaatt gatgccggct tggcacgtcg attgcagaaa gacatgccga cggcacgaag
    10201 caactgctag atggggtgct ccagcagggg cgccgggata caggaagatg cgacgggccg
    10261 tctactagag ggcgatacca tggaggctgc gtactcctct ccccgatgga ccttcgggtg
    10321 tgttggggat gggtgccttc ccctgagtgg ctcgccaagc cagtgaatca tagcccttgc
    10381 ccttagcgtg attccgagca gtattgctct ctaggaaaag caagaaggag gcacttcggt
    10441 gcctccttct tgtttttggg ggagagtgca ctccgtcgtc acgccgaatc cgcgccctcg
    10501 ggaatcacag gcggcaccac gaccattttc tcgcgctcgc acgtcacctg ccccggcctc
    10561 gcgccgcgaa acttccgcac gtactttcgc tgggtgtaga tcaccggcac cagcgagctg
    10621 tgtttggcct tcgagaagta ggccgcgatg gacgcggtgc gttcgatcac acgcgcgggc
    10681 ggcccgtcgc ttccccctcg cgatctcaag accacgtgcg aacccgggac tccttgggcg
    10741 tggaaccaga agtcggtcgg cttggccacc tggaacgtga tctcgtcgtt ttcgaagttg
    10801 ctccgaccca ccagcacgaa ccagcgcggg tcgacgtcga aggtgcggta cgtcttctcg
    10861 gcttcgcgct tcttcggagc gccctgtggg cgcggcgcgt cgtcgatcgc gatgtcgaac
    10921 ttggcccgga tggcttcgat cgttttcaag gcctcgttga acgagcgcga cccctccagc
    10981 accgccaacg aagcgcccaa atcctggtgc tcgcggcgca cgagctcgag gcggcgcacc
    11041 gcgtgctccg cgcttcgctc gagtttggct gcgcgccgga accgcttctc gatctggagg
    11101 tgcagcgact cggcggggtc gatcgcaatg gtacgaatcc gggacgcgtc gtacacgtcg
    11161 ggaagctcga tcgacgccgc ccccgcgggc acgcgggtct ggaacgcggc cagcgtctcc
    11221 gcctcgcggc gtagctcgcc atgctgcgcc gcgcgcgcga tgtcctcctc cagcttgacc
    11281 gacagctttg cgagcgcatc gacgcgacgc cgaagcggtc gcgtaatacg aagcaatatg
    11341 gtcgcgtggg caccttcgag aacgcgcgcc cccaacgagg cgcacgcggc gcgtgcgcta
    11401 tcgaaggggc cgaggacgtg cacgtcctct tcggcgttgg tcacatcgtg cgccggcgtc
    11461 gcgccgccga gatttccgtg cacgaccaga cgaaacggtg tctcgagcac ggcgacggca
    11521 gtgggcggcg ccgaggcgga cgggacgcgt cctttgccga gcgtctccac cgtcgacgtt
    11581 ccgcgtacga gagaagcgct tcccgccgac ccgaacaacg tgatcaagag gctcacgtgg
    11641 tcgccgtcgc gcgtctccgc gtcgacgcga agcacgcgcg cctcgccgag acggacgtcg
    11701 gtgatagtgg cacccgagag catcctcatg cgtgcgggtg cctgggcacc ttcgaacggc
    11761 gaatcgccga ccacgtagca gaacggaccc ggcgactcga ggaccgcgac cagatgcccg
    11821 cgtccgtaca ccggaatcgc gagtacgggc gcgaaccacg agggcgtgcc caggggacga
    11881 cccacgacgt cgtcgcggat ctcgcggacc aattttgcca gcagcgaggc gttcatttga
    11941 cagcgggttt gcgcgaccta tagtatagaa cgcgccggca acttcaaaca cgcaaggatt
    12001 cagaagaatg ctcatcagca tgaccgggtt cggacaaggg gaggcggcca acgcgcattc
    12061 gaccgtgacc gtcgaggtgc gatcggtcaa ccaccgattc ctcgacatcg cgttcaagct
    12121 cccgcggtcg ctccaaaacc gagaaccgga cataaaagag atcgtgcgcg gcaagatcgc
    12181 gcgcggacgc gtgtcgatca cgatctcggt cgagtcggcc tccacggggc gcggggtcgt
    12241 tataaacgaa atcgtgctcg aacagtacct cgcgcagctg cgcgcgttcg cgaagcgcca
    12301 cgacctcgac gagcgcatca gcctggatgc gctggtgacg ctgccggagg tcgtcaccac
    12361 caaggagacc gaaccgggtg aagacgagct gtggccgctg gtcgacaaga gtctcggcga
    12421 agcgctcgac gcgtgcaacc gcatgcgctt cgacgaaggt aaggcgctcg agaaggacct
    12481 ggtcgatcgc atgcgcctga tcgacaagac cgtggccgcg atcgaaaagc tcgcccccgc
    12541 ggtcgccaag cggcaggtcg aaacgctgcg caaacgcgtc gctcaactgg ccggagaagt
    12601 tgccgtcccc gaagaccgca tcgccgccga gatcgtcatg ctgtccgatc gcaccgacat
    12661 caccgaggaa gttacgcgcc tgcgcagtca catcgcgcag ttcaacggcg cgctcaagga
    12721 cggcggcgaa gtgtcgaagc ggctcaccta tctcctgcag gagatgcatc gcgaggcgtc
    12781 gacgatgggt tcgaaggcct ccgacaccga agtcatccag cacgtcgtcg tcttgaaaga
    12841 agagaccgag aagctccgtg agcaggtcca gaatctggag tgatcttggc gcgagcctcg
    12901 agcgctttcc tcatcgtcct ctcgggcccc tccggcgtgg gaaagaccac cctggtcgat
    12961 cgcttgctcg cggacgaccc gctggtgcgc cgatccatct cggcgaccac ccggacccac
    13021 cgggcgggtg aagtcgacgg gcgcgattac ttcttcgtga accgggaacg cttcgaggcc
    13081 atgaaggccg gcgatctggt cgagtgggcc gacgtccacg gagcgctcta cgggactccg
    13141 aggcgtttct tggaagggga actggccgcc ggccgggacg ttatcctcaa cattgacatc
    13201 caggggggag atcaggtaaa aaggtccttt cccggcgccg tcatggtctt catattaccg
    13261 ccgtcctttc gggtcctcga ggatcgcctc cgcgcccggg gggacgtctc cggtgatggc
    13321 ttaaaggtcc gcctcgccaa cgcccggaaa gagatcgagg ccggcgcgcg ctacgactac
    13381 atcatcgtca acgactcggt cgagcgggcg gccgccgagc tagcggcgat cgtcaccgcc
    13441 gaacggtgcc gccgccagcg aatggccccc gggttcatcg ccgatctgtc gaaccaacag
    13501 gcgaaataac aggagtgtcc cgaatgtcga ttcccatcga gaaactcgtc atcatcgaga
    13561 agatcaccca gaagatccag aaccgctacg aggccgtgcg cgtcatggcg cgcgaagcgc
    13621 gtcgcttgaa ctcgctcgtg attcgcggcg cggagccgga gccggatttc aagcccacgt
    13681 cgaccgcggt caagcgcctc atcaacgaca agatccagtt ctcctacgcg gacgaaccga
    13741 cgggcagcgg cgacttcttc caggagtcgt agccgccgga tgtgagcctt tccggtaaga
    13801 agattctgct cggtgtcacc ggcggcatct ccgcctacaa gtccgtattc ctgctccgtc
    13861 tgttcaagaa ggcgggcgcc gacgtcaagg tcgtcatgac cgaagcggcc gccgagttcg
    13921 tgacacccct caccttcgag tcgctctccg agcatcccgt ccacattcgc atgttcgagg
    13981 gcgaagggag gtcggccggc gcgatttctc ctgtcgagca catcgatctc gccaagtggc
    14041 ccgacctggt cgtcgtcgcg cccgcgaccg ccaacacgct cgccaacttc gtgcacgggc
    14101 gcgcggacga cctgctcgcg accatcgtga gcgcgtacca aggtcccatc atgctggccc
    14161 ccgcgatgaa cgacgtcatg tgggagaacg cggccaccca ggagaacatg cgcatcctct
    14221 ccgaccgcgg cttccgcgtg gcaccgcccg agaagggtga cctcgcgtgc ggctacgaag
    14281 cgaccgggcg catggccgag ccggagacga tcttcgcagc ggcgcagacc ttgttctcgt
    14341 cgcccttgcg aggcaagaaa gtgctggtga gcgtgggcgg gaccgaggaa gacatcgacc
    14401 cggtgcgcgt catctcgaat cgatcgagcg gcaagatggg cttcgcggtg gccgaggccg
    14461 cgcgcgacgc cggcgccgac gtcaccgtgg tggccgcgcg cacgtcggtg gccgcaccgc
    14521 acgggttgcg catcgtgcgc gttcgcacgg gcgacgagat ggcgaaggcg ttgaaggccg
    14581 agttcaagaa cgccgacgcg ctgttcatgg tggccgcggt cagcgacttc aagcccgcgt
    14641 cgaccatggc gcagaaaaag aagtccggcg aaggctggac gctcgagctt tccaagaccg
    14701 acgacatcct cgcctccctc ggcgccgaca aaggcaagcg cgtcgtggtg ggttttgcgc
    14761 tcgagaccga ggacgaagag cagaacgcgc gcgccaagct cgcgaagaaa cactgcgact
    14821 tcgtcgtctt gaacaagccc ggggacgcct tcgggctcga caccaacgtg gtgtcggttt
    14881 tcgacgccac gggtgcggtc taccagagca aggcgccgga gagcaagcgg gagatcgcgc
    14941 gcaaactcct cgatctgatt gccggctcga aacgatgagc gctcgcgatg gcgagcaccc
    15001 gccctcgtca ctcgagcagc acgatcttct tggcttcggt gaaggtgccc gcgcgcatgc
    15061 ggaggaagta gactcctgag ccgaggcgct cgcctctcgc cccacggccg tcccactgga
    15121 catcgaaacg gccagcttcc cgatggtcat cgacgagcgt ctgcacgtgt cgccccgtga
    15181 cgtcgaatac ggctatgttg acgtgccctc cgcgcacggg aacgtcgtag cgaatcgtgg
    15241 tcgtggggtt gaacggattg ggaaagttac ccaccaacgc gaacccgctc ggcatttgtt
    15301 cgtcgtcgac ggcggtcggc ggtgtaacca cccccaagcg caggatccag tcgccggtga
    15361 ggttaagggg agggttctcc gtgtaggacc aggtagtgcc gttcctgaag aagttacgct
    15421 gagcggtcat gccactggta tcgtaggcga acccagggga agaaaacaac acgtcgatgc
    15481 cgactcggat cgtgccactc gtaatcatcg gaaccgtacc cggcggaatg acgatatcga
    15541 tgaactgggc gccacccggt gttcctgaaa acaggtaggt gtcgctggat agaaacagaa
    15601 gtgtgccggg gtccaggttg ccggcgtctt gccagatggt aaacgtgatg atcccgtcgc
    15661 cggccccgca gtgcaggatg cggatgcgct cgatctcgta cggataccac gtcggggccg
    15721 cggtgaacag agccgcaaat gccttttcgt cgatgacggc aaagctgcac ccgacgctct
    15781 gtccctcctc gaagtcgtcg ttttggagcc agccgtactg cgcgctcgct tggctggccg
    15841 ccgtcacgag tacacaatag acgagagttc ggaccaggat gcgcatgggg ttgtcctttc
    15901 tgccctcgcc gccttgcgag atgcatcaat catacactaa acgagcgggc cacgtgcggc
    15961 atcgaacccg cgcttgcgtt tcatcgctcc aagccatagg ctgccagtat gtccaatcgg
    16021 aatcgcaacg cgacagtggc cgcattaagg cgcttggaga gcaaaggcgt tcggtcggtt
    16081 tatctcgaag ggatcgccgc gtccgtaccg atgaccgaga acaggaccac gtcgacgcgt
    16141 gtggcaaccc ccgcattacc ccccggactt tccggtctgg ctaagaccgt ctccgtctgc
    16201 acgcgctgtg acctgcacaa gagccgcacc cagaccgtct tcggtgtcgg caatcccaac
    16261 gcgaagctca tgttcatcgg cgaagccccc ggcgccgagg aagacgcgcg cggcgagccg
    16321 ttcgtcgggc gcgcaggtca gttgttgacg aagatcatcg ccgccatggg catgacgcgc
    16381 gacgaggtct acatcgccaa catcttgaag tgccgcccgc cgcagaaccg agacccgctt
    16441 cccagcgaag tcgagtgctg cgaagaatac ctgcgtgcgc agatcgcgat gatcaagccg
    16501 aagtacatct gcgcgctggg gcgcatcgcg gcccactggc tcttgaagac cgaagcgccg
    16561 ctgggcgcgc ttcggacggg aagctacacg tacgagggca tcccggtgat cgtcacgtac
    16621 cacccggccg cactgctgcg caaccccgcg ttcaaagcgc cgtgctggga agacatgaag
    16681 aagctgatgg cattgttgaa agacgcgtga ttgcagtaag gccctcggca ccgatgcgtg
    16741 cgaacctctg ctaagatgat cggccaaacc atctcccact atcgcatcca ggagcgcctc
    16801 ggtcagggcg ggatgggcga agtcttcctg gccgaagacc tgcggctgca tcgctccgtg
    16861 gcgttgaaga tcctgcgcca gcgtctcgga tgcgaagacg aagagcgcgc gcgcatcatg
    16921 cgcgaggcgc gcgcagcttc ggcgctcaac caccccaaca tcgccgtcat ctacgacgtc
    16981 gaagagaccg agacgcccga cggccgcgtg tacctcctct cgatggagta cgtgcgcggc
    17041 aagacgctcg cggatctcgc ggccggctcg tcgctctcgc tggacgacat cctcgacatc
    17101 gtcgcgcaaa ccgccgacgc gctggcggaa gcgcacgcgc gcggggtcgt gcaccgcgac
    17161 gtcaagccgt ccaacctgat ggtggcgcag gggcgcgtga aggtgctcga cttcggcttg
    17221 gcgcagatgc agccgcggct gcgcgccgac gacctcacct ggagtcgcga cgcgacgcat
    17281 gcccccgagg gcgtcttcgc cgggacgccg cactacatgt cgcccgagca ggcgctcggt
    17341 cgcacgctcg actcgcgctc ggatattttc tcgctcgggg tggtgttcta cgaactgctg
    17401 gcgggcaagc gccccttcga gggcgacacc tttgtgcaaa tcgcggacgc gatcctgcac
    17461 cgcgacccgc cgccgctccc ggtccgtttc tccgacattc gcgcgcccga ggtggagcgg
    17521 ctgatgacgc gcatgctggc caaggacgcc aaggctcgcc cgcgtgacct gcgcgaaatc
    17581 gtcgtcgagt tgcagcggct gcgcgcgggg gcgtcgccgc tcacggcgca gtcgggcgaa
    17641 ctcacggtgg ccgtggcggg cttcgccggg atcggcgagc gcgcggaaga cgagtggctg
    17701 ggcacgggat tgtcggaaac ggtaacgacg gcgctgcaag aggtcgaagg actggtggtg
    17761 tgggggcgcg agcaactgcg cgagagtctg cggcgtctgg gcgtcgaagc cgccgagctg
    17821 gcgccggaag acgcggtgca attgggacgc atgatcggtg cgcgctgggt gctttccggc
    17881 gcgtaccaac gactgggcga gcaagtgcgc gtgaccgcgc gcgtggtcga agtggagtcg
    17941 ggtcgtatca tacgcgcggt gaagcaggac gggcgcctgg atgcgatctt cgacttgcag
    18001 gaccgcattg tcaccgagtt ggcctccggg ctgcgcggca gtgtggcggc cgcgcacgag
    18061 ggcgaggaga ccaagatcgt cgcggcctac gaagcgctcg cgaaggggct gctcaatgtt
    18121 cgcgccgaca gctacgagtc gctggaccgc gcgattctct ttttcgagcg cgcggtggca
    18181 ctcgaccccg actacgtgcg cgcgcagatc gagctggccg ccgcgctcga gcagaagggc
    18241 aattatctga ccgcgcccga gctgcacgag cgggcggcgg cgattctgcg caaggtgctc
    18301 gaggtgcgcc cgcgcctggc gcgcgcgtgg cgcgagctgg ggatgtcgct gatctcgctg
    18361 ggccgcgtgc gggatggggt ggagaacctg cggcgcgcgc tctcgctcgc ccccgacgac
    18421 gcgcgcacac aggcgggctt ggcgcgcggg ctattcatcg gtatggccga cttccgcgcg
    18481 gccgccgaga tgttcgaacg ctcggtggaa cgcaacccgg aggcgggctg gtactggatg
    18541 cagctcgcgc attgttgtgc gttcctgcgc gagttcaagc gcggcgagaa ggccgcgaaa
    18601 cgcgcgatcg agttgcagga ggcatttctc tccggacagc agggcgtgca gctggtcggc
    18661 gcctatatgc ggctggggca cttgctcgcg ctgcaaggcc ggccgcgcga cgcccacgac
    18721 gcgaacgcga gcgagagcct cttcctcgaa aagatcgatc acgcgctgcg ctcgcgcatt
    18781 cgcatcgaac tgcagatgcg cctgggcgcg tcgctgatcg atctcggcga ccagcgcggc
    18841 gcgcgtgcag ccttcgaagt cggactggat gcgttcacgc aacgcctggc gctcggcgcc
    18901 gacgacccct ttacgcgcta ttacgcggcc gccatccatg ccctgctggg gcaagacgac
    18961 gaagcgatcg cgctgctcga gagggccgtg accgcgggca cccgcgcctt cgtgatcgcg
    19021 cgggcccgta tcgagccgga gtggaatggg ctgcgctcca atccccgttt cgcacgactg
    19081 accgcgtagc aaggaggcgt cgaatgccta gcaccgatcc caatctaccc gtcgaaggct
    19141 ggctcgacat cggagccgcc gccgagctat cgaagcgcgc cgtccaacaa gtgctcttgg
    19201 ggaccacgaa ggtggcgctt acgtgcaagg acggcgagtt cggggcgatc agcggcaact
    19261 gcaaccacgc cggcggtccg ctgggcgaag gaaccatgga gggcgactac gtcgtgtgcc
    19321 cgtggcacca gtggcgcttc catcgcagcc agggtgtggg cgagcccggc ttcgaggagg
    19381 acgcggtgcc cgcgtaccac gtgcgcgtgc gcgaagggcg ggtggagatt catcccaagc
    19441 cgtacaccaa gcgcagcaag aagccgcacg atccgcaccc gttgtcgcgc gagattcgcc
    19501 gcgaggacgg gcccattcgc gtggtcggca tctcgaccac cgtgatgaac caggactacc
    19561 cgcgctactc gacttcggac gcgctgctgg aagaagccat tcgccacgcg gatgcgacct
    19621 ctcacgagac caagctcatc aagctgcgtg agctttcctt ccgatcgtgc gaaggctact
    19681 actcgaagtc cgcgcacgcg tgcacgtggc cgtgctcgat cacgcaaatg gaccccaacg
    19741 accagctcga ccgcgtgtac gaagccttcg tccactgggg cgacgtgttc gtgatcgcga
    19801 cgcccattcg ctggggcaac gcgagtcatc tttattacaa gatggtcgag cgcatgaact
    19861 gcatccagaa ccagatgacg atcgccaacc gcacgctgct gcgcgacaag gtggtgtgcg
    19921 cgatcgtcac cggtggccag gacaacgtgc agggtgtcgt gggccaggtg ctgggcttct
    19981 tcgccgagct ggggtgccac ttcccgcagt acccgttcat cgcccactcg cgcgggtgga
    20041 gcgcggagga catggaggcc aacgtgatcg cggtccagca cagcgaagag ctccgtgcgg
    20101 gtgcgcgcgg cctggtcgac cgcggcatcg agctggcgga gcgcctgctc gcgacgacac
    20161 cgcacaaagc cgagcggggg ggtcgcaagg gcaaggcgct gcgggattag cggccctgtc
    20221 cacagaatcg aagcccttat ccaccgcttg tccacagcgc gtccacagct tcccaaggga
    20281 ttgtttcctg tctcccaggc gcgtgagtta gaatccggcc acgaaggaga aaccaccgtg
    20341 gccgaagcta ttcgtaccgc acagactcaa ctgcaagcgc ccgggcgcgt cccgccgcag
    20401 tcgctcgaag ccgagatggc cgtgctcggc gccgtgctgc tcgacaacaa cgccttctcg
    20461 atcgcgaccg agagcctcag tagcgacgcg ttctacaaga aagcccacaa ggacatcttc
    20521 gccgcgatgg agggactctc cgcgcgcggc gaagcgatcg acgtcgtgac actctccgag
    20581 gaattgaagc aacgcgggac gtttcagtcc tccggcagcg caacttatct cacgcagatc
    20641 atggactcgg tccacaccgc ggccaacgtc gagtactacg ccaacatcgt gctcgagaag
    20701 ttcgtgatgc ggcgcttgat cacgatcagc aacgacatcg ccacccaatg ctacagcggc
    20761 gaacgcgagg cctccgagct gctggacgaa gccgagaagc acatcttcga gatctcgcag
    20821 cagggaatgt tcaagggctt cgaaccgatc gggaagatcc tccgcgatca tttcaagaac
    20881 atcgaggcgc tgtatcaaag cggcagccac atctccggta cgccgtcgcc cttcgaagaa
    20941 ctggattcgc tcacgtcggg cttccagaag tcggatctca tcatcgtcgc ggggcgcccc
    21001 ggcatgggca agacgagctt cgcgttgaac ctgtgccagc acctcgctat caaggagaag
    21061 acgccggttg gcatcttcag tctggaaatg tcggccgagc agctggtgac gcgcctgttg
    21121 tgcagcgagg cgcgcatcga ctcgaaccga ttgcgccgcg gctacttgaa atcgaacgag
    21181 tacgcggaac tggcgatcgt ggcgggttat ctggcggaag cgcccatcta catcgatgac
    21241 tccgcggggc tctccacgct cgagctgcgc gccaaggcga gacgcctcaa ggccgaggcg
    21301 aacatcggcc tgctgatggt cgattacctc cagctgatca gcgtgcgcga gcgcgtcgaa
    21361 aaccggcagc agcagatctc gctcatctcg cgcggactca aagcgcttgc gaaggagctc
    21421 aacattccgg tcatcgcgct ctcacagctc tcgcgtgcgg tcgagtcgcg cggcggcgac
    21481 aagcgcccaa tgctgtcgga tcttcgcgag agcggaagta tcgaacagga cagcgacctc
    21541 gtcctcttcc tgtaccgtcc cgaagtctac gacaccagcg acgagaatcg cggcaaggcc
    21601 gagctgatca tcggcaagca gcgcaacggg ccgacgggga ctgtgaatct caccttcatc
    21661 gattcctgca cgcgcttcga gccggccgcg ttcaccgaat aatatggacg tgtccgcccc
    21721 gcgcacgacc atcacgcagg cgttgcgcga tctgggcgcc accattctca atttcctccg
    21781 cgacgtgggc ggctcgtcga tcatgctggc gggcgtgttc gcgcgcttgc gcgagctacc
    21841 gcgcaccgcg gagcagaccc tgctgcagat gcagttgatc ggtatcggct ccatcccgct
    21901 cgtgctcgtt acctcggtgt tcgtgggtgc cgtggccgcg gtgcaagccg cgtatcaatt
    21961 ccaggactac gtccccatgg tgttcctcgg caccgtggtc ggcaagtcgg tcatcctcga
    22021 gctcgggccc gtgctcaccg cgttggtggt gggcgggcgc gtgagcgcgt cgatcgccgc
    22081 cgagctcggt accatgaagg tcaccgagca aatcgacgcc atggaagtcc tcgccatcga
    22141 cccgctgcgc tacctggtaa tgccgcgggt ggtcgcgtgc gtggtcatgc tgcccgtgct
    22201 caccatcttc gcggacttcc tcgccatcct gggcgggatg ttcgtcgcga acgcgaccgt
    22261 cggcgtcgac tacgccacct tcgtcgaggg gctgcgcctg ttcttctacg tcgacgacgt
    22321 cattagcggc gtcatcaagg ccgccacctt cggcgggatc atcggcctca tgggctgcta
    22381 caacggcttt cgcaccttcg gcggcgcgca gggtgtgggc cgggccacca tgcaggccgt
    22441 cgtctcgagc tgcatctgca tcctcattac caactacttc ctcgcctcgg tcctcctgcg
    22501 ggtcattttc tacaagggcg actagtttcc gcgtgacggt gcggctccgc atccgtccta
    22561 tcttccaacc cgattccatc cgcaggagga cttcgcatgc ttcgaccccg atggaccacc
    22621 ctagcccttg ttacgacgac acttgccctc ggcctccacg catcgctcgc ccacgcagcc
    22681 tggcagcaga acggcaaccg ggtgtcggac gtgtcgcttg gacaggcccg ccctgccatc
    22741 gcgcccgacg gcgaaggggg tgcgatcgtc gcgtgggtgg agaatcgaag cggcctgttc
    22801 cttgatatct acgcccaacg gctcgattca tggggacgcc cggtttgggc gacgggaggt
    22861 gtcgccatcg ccgtcgtggc gtgggaccaa gacaacccca tcgtggtgag cgacggcgag
    22921 ggcggcgcca tcatcgcgtg ggaggactcc cgcaacggca cggactccga catctttgcg
    22981 cagcgcgtga gtacggccgg cacgccgatg tggccgccga acggggtcgc ggtgtgtgaa
    23041 caaccgttcc accaaaacaa actgcgcatg acgcgctcgg atctcaacgg cgtcatcatc
    23101 gtgtggcagg acgtgcgggc gaatcccgac tggaacatct acggccagcg cctcgacggc
    23161 ctgggaatag cgcaatggcc ggccaacggc atcgcggtgt gcgcggcggt aggtaaccag
    23221 ttcgaccccg acatcgtgag cgacggcttc ggcggtgcca tcgtggcctg ggaacaccag
    23281 gtcggtttcg actatgacat tcgtgcgcag cgcgtccacg tgtcgggtgg agtgatgtgg
    23341 agcctcgacg gtgagtcctt gtgcagcaac cttgccaccc aaggggacgt gaagctggtg
    23401 agcgatgatg ctggtggtgc gatcgccgtg tggtccgact ctcgtcagtc cccggcagca
    23461 ctattcgcgc aacgcatcgt gcaatacggc tacacctggt ggctatccga cggcgtggaa
    23521 gtgacgacga ccaccggcga ttacgagacg gattatcagg tcgtcgcgga cggctcgggt
    23581 ggggtcatcg tcgtctacaa tcgcgctttc gccctgatca tggggcaacg aatcaacccg
    23641 ttcggctcgc ggatgtggac ggcctccggg atgaccgtta gtccggcgct tatccaggaa
    23701 atgagtccgc acgttgcgag cgacggactc ggcggcgcca tcgtttcgtg gatcgattat
    23761 cgcaacgtcg tccccgacgt cttcgcgcgc cgcgtggacg caaccggcgt cccggtggga
    23821 ccggcgggcg gcacgcccat tgcggccgtt ccagggggcc aaatgccagc gcccatggtc
    23881 agtgatggcg ccggcggcgc catcatggcg tggcaggact accgcgccat tgactacccg
    23941 cacgtgtacg cgcagcgcat ggatcgcctc ggcaattggg gctacccggc aggtgacatc
    24001 gtcagcgcgc tcgacgttcc cggcgaccag ggcggcgtga tcaacctgca atggacggcg
    24061 agtcgcttgg atccgtggcc caaccaggaa cccgatcgct acaccgtgtg gcgcgcgatc
    24121 gatcccacgc tggcctccgt gttcgcgagc tcggggaccg cgctcgtgtc gccgggcgaa
    24181 ctggatccgt ccgcgaccga agcgctgcgg gtcgagacca tcgcgggaca gacgtactac
    24241 tggcagctcg tgtccaccgt cgacgcgttc tatctgaact cgtacgcgca agcggtgccg
    24301 accctgttcg ataacacgat ctcgtcgccc ggcccgcact acttccaagt gatcgcgcac
    24361 ggattcacgc cgcaggagta ttgggtgtcg gcgccggcga gcggcgactc gttcgacaac
    24421 ctggcgccgg ccgcgcccat catgctcgcc gcccagcgcg tgggcaacga cgtcgaactc
    24481 acatggaatc cctccggcgc gaacgagctg gacttcgccg aatacgcggt ctatcgcgcg
    24541 tcgtccgcgg gtgtgcagcc gatacccggc ttcttcgtga cgggaacgcc ggacacggtc
    24601 ctcgtcgata gcagcccgcc gcccggctac ctctattaca tcgtgaccgc gcgcgacgtg
    24661 cacgacaatc agagcgctcc ctcgaacgaa gcgagcgtca ccattcccac cggcatcggc
    24721 gacgacgcgc ccgccctcac gcgcctgacg ctcgatggga acttccccaa tcccttcagc
    24781 cgcgacaccg agctgcgcat tggtctcccg cgtggttccg acgtgcgcct cgaagtgttc
    24841 gacgtggccg gtcgtcgggt cgcgacgcgg gatgtcccgc gtctttccgc gggctggcag
    24901 cgcgtcgatt tcgatggacg cgacgcgcgc ggcaatccgc tcgcgagcgg cgtatacttc
    24961 ggtcgcatca ccgcggccgg cgagacacag accatgaaga tggtgattcg gcggtagcgc
    25021 cagcgctctt cgacgaccca tatgcaagcc ccggtcggcg tcatgccggc cggggctttc
    25081 tcgtgtgcta gaatgccgcc gatggtcacc atcgagtgca aggacgtgtg gaagagcttc
    25141 ggacgcaaga gtgtgctgcg cggcttgaat cttacgatcc agcccggcga gacggtcgtc
    25201 atcatcgggc agagcggcag cggcaagagc gtgctcttga agcacatcgt cgggctcatc
    25261 tacccggaca agggagcgat cctgatcgac gggatcgaca tcacgaagct caatcgcaag
    25321 aaactcttcg agcttcgcat gggctttggt atggtcttcc agggtgcggc gctgttcgac
    25381 tcgtacacgg tggaagagaa cgtgggtctg gcgatgcgcg agcacacgga gctttccgac
    25441 gaagaagtga atcgcatcgc ggaagagaag ctgcgcatgg tcggcttgga cggtatcggc
    25501 gacaagcacc cggccgaact ctcgggcggc atgaagaagc gcgtgggctt cgcgcgcgca
    25561 atcgcgatgg agcccaagtg cgtcctgtac gacgagccga ccacggggct ggacccgatc
    25621 aacgcggacg tcatcaacaa tctcatcgtg aaactctcca acgagctcca catcacctcg
    25681 atcgtggtga cgcacgacct cgccagcgcg tacaaggtgg gcgaccgcat cgtcatgctg
    25741 cacgacggta aggtgctctt cgatggaacg ccggaacaag tgaagatgac gaccaacgcc
    25801 atcgtgcgcc agttcatcga aggccgcgcg gaaggtccca tcgtggtcgg gagcggagaa
    25861 tgacgagccg cgggctcgag attcgtgtgg gcgcggtggt catcgccgcc gcgctggtcg
    25921 cggtcatcgg taccatgtgg ttccagaaat tccagctcgc ggagaagcgc tatccctttt
    25981 atgtgcgttt cgacgaagtg ggcggtctgg tgagcggcga cccgattcag gtcaacggcg
    26041 tcgagcgcgg acgcgtcgac cgggtcgatc tgctcccgcg cggcgtggtg gtccagatgg
    26101 cggtgcgcga gggggtcgtc attccgctcg actcgcagat cgcgttgaag agcatcggca
    26161 tcatgggcga gcgattcgtg gccatcgtgc agggcgattc gtcggtcgcg atcgcgcccg
    26221 gcgacacggt cgacggctcc tacttgatgg gcttgagcga agtgatgggt gcggcgggcg
    26281 gcattttgg
//
LOCUS       DASPGK010000414         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_685406, whole genome shotgun sequence.
ACCESSION   DASPGK010000414 DASPGK010000000
VERSION     DASPGK010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685406"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VEC56_11440"
     CDS             <1..708
                     /locus_tag="VEC56_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14808.1"
                     /translation="EAPAVAATKKLLAFANDVDSFHVHEREVYWLCRKKFSESKFSGA
                     QLERTLAMPTTLRNVNTVRRIAAKFALALMLVAAPLAARADSPPPPPFTSAEGVVREL
                     YRLVTVEKGQETDWEAARALFLPEAVIVLRVSKDASQVFDVEGWINDFKAFNEKARVK
                     ERGFSEKILVLKPRVFRDIANVFVLYEAAITDSERPPTRGVDSIELIQKDGRWWIAAI
                     TNDLPNADNPIPAELQE"
     gene            712..2604
                     /locus_tag="VEC56_11445"
     CDS             712..2604
                     /locus_tag="VEC56_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXV14809.1"
                     /translation="MTDAGKAARTAPSRFSTESLIALLAIVAIAVHLVMRYALRVTGE
                     VRGLPQYEIPLVVALFLGGLPLVAGLARNLLRRQFSSDLLAGISIVTSVILGEYLAGT
                     FVVLMLSGGEALESYAVRNASSVLNALARRVPSVAHVKHDGDMRDTALDDVAVGDTLV
                     IFPHEICPVDGTVIDGHGSMDESYLTGEPYVMSKVPGSGVLSGAINGESALTIRADKR
                     AVDSRYAKIMQVMRESEQRRPKLRRLGDQLGAVYTPVAVGIALVAWAATGDPIRFLSV
                     LVVATPCPLLIAIPVAIIGSISLAARRGIIIKDPAILEKIGTCRVAMFDKTGTLTYGE
                     PKLTEVVAADGFVRADVLALVASLERYSRHPLAGAIVEGAKKEGLALRDAQEVSERPG
                     EGLRGVVDERRIQVTSRKGFVARHPDEADRLPPLAGGMECVVLLDDRYAATFHFRDEP
                     RADGVPFISHLSRKHGFERVMLISGDRESEVRYLADKVGIKEVHASQSPEQKLALVRE
                     ETTKANTVFLGDGINDAPALTAATVGIAFGQGSDITTEAAGAVILDSSLQRVDELMHI
                     GRRMRTIALQSAVGGMVLSVVGMLVAAAGYLPPVAGAVTQEAIDVFAVVNALRVAIKP
                     KALTDY"
     gene            complement(2591..2971)
                     /locus_tag="VEC56_11450"
     CDS             complement(2591..2971)
                     /locus_tag="VEC56_11450"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXV14810.1"
                     /translation="MRVLFVCVENSCRSQIAEAFARMHGAGVDAHSAGSKPSGKVNEK
                     AIASMREIGYDLSTHRSKGLDEIPPGPFDVVVTMGCGDACPWVPATRREDWAIPDPKE
                     MDPDGFREVRFEIGRRVDALLSSR"
     gene            3031..>4055
                     /locus_tag="VEC56_11455"
     CDS             3031..>4055
                     /locus_tag="VEC56_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXV14811.1"
                     /translation="MLLLKDIFVIFGLAVAVALACHRLKIPTVVGFILTGVIAGPHGL
                     GLTTEIDAVERLAEIGVIFLLFTIGLEFSVASIGHVKRLFFVAGPLQVVLTALVVFAW
                     AVAAGVEVPRAILFGLLASLSSTAIILRLLQERAELSSPHGKGTMAILIFQDIAFVPM
                     MLVIPFIAGIGGENPWREVGILVLKVAIIWLVIGLAAPRLAPRVLTRIARTQSNELFL
                     LSIGVVCFFVAWLTSLAGLSLALGAFLAGLILSDSEHSHRALENVLPFRDVFSSFFFV
                     SMGMLLDAGYVADHLPAVLATTLVVMVLKAAVSTGVILAVGLPYRVALLTGLALSQTG
                     EFSLLVL"
BASE COUNT          617 a         1357 c         1341 g          740 t
ORIGIN      
        1 gaggcgcccg cggtcgcggc gacgaagaag ctcttggcgt tcgcgaacga cgtggacagc
       61 tttcatgtcc acgagcgcga agtgtactgg ctgtgccgga agaagttcag cgaatcgaag
      121 ttctccggcg cccaactcga aaggactctg gccatgccga ctacccttcg caacgtgaat
      181 acggtccgcc gcatagccgc caagttcgcg ctcgcgctca tgctggtcgc ggcaccactc
      241 gcggcccgcg ccgattcgcc gccgcccccg ccgttcacga gcgccgaggg cgtggtgcgc
      301 gagctgtatc gcctcgtcac ggtcgaaaag gggcaggaaa ccgactggga ggcggcgcgc
      361 gcgctgttcc tgcccgaagc cgtcatcgtg ctgcgcgtct ccaaggacgc ctcccaagtc
      421 ttcgacgtgg agggctggat caacgacttc aaggccttca acgagaaggc gcgcgtcaag
      481 gagcgcggct tctccgagaa gatcctcgtg ctcaagccgc gcgtgttccg cgacattgcc
      541 aacgtgttcg tcttgtacga agccgccatc accgactcgg agcgaccgcc cacgcgcggc
      601 gtcgacagca tcgagttgat ccagaaggac gggcgctggt ggatcgcggc catcaccaat
      661 gatctgccca acgcggacaa tccgattcca gccgagctcc aggagtagcg aatgacggac
      721 gccggaaagg ccgcccgtac cgctccctcg cgtttttcca ccgagagcct gatcgcgctg
      781 ctcgcgatcg tcgcgatcgc cgtacacctc gtcatgcgct acgcattgcg cgtgaccggt
      841 gaggtccgcg gtcttccgca gtacgagatt ccgctggtcg tcgcgctctt cttgggtggc
      901 ctgccgctgg tcgcgggtct cgcgcgcaac ctcctgcgcc gccagttcag ctcggacctg
      961 ctcgcgggca tctcgatcgt cacgtcggtg atcttgggcg aatacctcgc gggtaccttc
     1021 gtcgtgctca tgctctcggg cggcgaggcg ctcgagagct acgcggtgcg caacgcgtcg
     1081 tcggttttga acgcgctcgc gcggcgcgtg ccgtcggtgg cgcacgtcaa gcacgacggc
     1141 gacatgcgcg acacggcgct cgacgacgtc gcggtggggg acacgctggt catcttcccg
     1201 cacgagatct gcccggtcga cggcaccgtg atcgacggcc acggctccat ggacgagtcg
     1261 tacctgaccg gcgagcccta cgtgatgtcg aaggtgcccg ggtcgggcgt cctctcgggc
     1321 gcgatcaacg gcgagtcggc gctcaccatt cgcgccgaca agcgcgcggt ggattcgcgc
     1381 tacgcgaaga tcatgcaggt catgcgcgaa tcggaacagc ggcgccccaa gttgcgccgc
     1441 ttgggcgacc agctgggcgc ggtctacacg ccggtcgcgg tcggtatcgc gctggtcgcg
     1501 tgggcggcga ccggcgaccc gattcgcttc ctgagcgtgc tcgtggttgc cacgccgtgt
     1561 ccgctcttga tcgcgattcc ggtcgcgatc atcggctcca tttcgttggc cgcgcgccgc
     1621 ggcatcatca tcaaggatcc cgccatcctg gagaagatcg gcacctgccg cgtcgccatg
     1681 ttcgacaaga ccggaacact cacctacggc gagcccaaac tgaccgaggt agtcgccgcc
     1741 gacgggttcg tgcgcgcgga cgtgctcgcg ttggtcgcga gcctcgagcg ttattcgcgc
     1801 cacccgctcg cgggcgcgat cgtcgagggc gccaagaagg aaggcctcgc gctgcgcgac
     1861 gcgcaggaag tgagcgagcg gcccggcgag ggactgcgtg gcgtggtcga cgaaaggcgc
     1921 atccaggtca ccagccgcaa gggattcgtc gcgcgccacc ccgacgaagc cgatcgactc
     1981 ccgcccctcg cgggcggcat ggagtgcgtc gttttgctcg acgatcgtta cgccgcgacc
     2041 ttccacttcc gcgatgagcc gcgcgcagac ggcgtgccgt tcatctcgca cctttcgcgc
     2101 aagcacggtt tcgagcgcgt gatgctgatc tccggcgacc gcgagtcgga ggttcgctac
     2161 ctcgcggaca aggtcgggat caaagaagtg cacgcgagcc agagtcccga gcaaaagctc
     2221 gcgctggtac gcgaggagac cacgaaggcc aacaccgtct tcctcggcga cggcatcaac
     2281 gacgcccccg cgctgaccgc ggcgacggtc ggcatcgcgt tcgggcaggg aagcgacatc
     2341 accaccgagg ccgcgggggc ggtgattctc gacagctcgc tccagcgcgt ggacgaactc
     2401 atgcacatcg gccgtcgcat gcgaaccatc gcgctgcaga gcgcggtggg tgggatggtg
     2461 ctgagcgtgg tggggatgct ggtcgcggcc gcgggctatt tgccgcccgt cgcgggggcg
     2521 gtcacgcagg aagcgatcga cgtgtttgcg gtggtgaacg cgctgcgggt cgccatcaag
     2581 cccaaggcgc tcaccgacta ctgagcagcg cgtcgacgcg ccttccgatc tcgaagcgaa
     2641 cctctctgaa gccgtccgga tccatttcct tggggtcggg gatggcccag tcctcgcgtc
     2701 gcgtcgcggg cacccacggg cacgcgtcgc cgcagcccat cgtgaccacc acgtcgaagg
     2761 gtcccggagg aatctcgtcg agtcccttcg agcggtgcgt ggagaggtcg tagccgattt
     2821 ctcgcatcga cgcgatcgcc ttctcgttca ccttccccga gggcttcgag cccgcgctgt
     2881 gggcgtccac gcccgcgccg tgcatgcgtg cgaaggcctc cgcgatctgg ctccggcacg
     2941 agttctcgac gcatacgaac aggacgcgca tgcacggaga ataaatcgtg gagcgatcgg
     3001 acgcaatggc gctagaatgc gcccgtgcag atgctgctcc tcaaggatat cttcgtcatc
     3061 ttcggcctgg cggtcgcggt ggcgctggcg tgccatcgcc tgaagatccc caccgtggtc
     3121 ggtttcattc tgaccggcgt catcgccggc ccgcacgggc ttggtctcac caccgagatc
     3181 gacgccgtcg aaagactggc ggagatcggc gtcatctttc tcttgttcac gatcggactc
     3241 gagttctcag tcgcttcgat cgggcacgtc aagcgcctct tcttcgtcgc ggggccgctc
     3301 caggtcgtgc tgaccgcgct ggtggtgttc gcatgggcgg tcgccgcggg cgtcgaggtg
     3361 ccgcgcgcga tcctcttcgg actcctggcg tcgctctcca gcaccgccat cattcttcga
     3421 ctgctgcagg agcgcgcgga gttgtcgagc ccgcacggca agggaacgat ggcgatcctc
     3481 atcttccagg acatcgcgtt cgtgcccatg atgctcgtga ttccgttcat tgccgggatt
     3541 ggcggggaga atccgtggcg cgaagtcggc atcctcgtcc tgaaagtggc catcatctgg
     3601 ctcgtcatcg gtctggccgc cccgcgcttg gcgccgcgcg tgctgacccg catcgcgcgc
     3661 acgcagagca acgagttgtt tctgctctcg atcggcgtgg tgtgcttctt cgtcgcctgg
     3721 ctgacctcgc tcgcggggct gtcgctcgcg ctgggcgcgt tcctggccgg gctgatcctc
     3781 tcggactccg agcacagcca tcgcgcgctc gagaacgtac tgccgtttcg agacgtgttc
     3841 tcgagtttct tcttcgtttc gatggggatg ttgctcgacg ccggatacgt ggccgaccac
     3901 ctcccggccg tgctggccac cacgctggtc gtaatggtgc tgaaggccgc cgtcagcacc
     3961 ggcgttatcc tcgcggtggg gctgccgtat cgggttgctc tcctgaccgg tctcgcgctc
     4021 tcgcaaaccg gcgagttctc gctgctcgtg ttgag
//
LOCUS       DASPGK010000415         6256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1270912, whole genome shotgun sequence.
ACCESSION   DASPGK010000415 DASPGK010000000
VERSION     DASPGK010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270912"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VEC56_11460"
     CDS             <1..1384
                     /locus_tag="VEC56_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXV14812.1"
                     /translation="AHPQTGPPQVARTMAVTDTGLRRVLGSASATAVVIGAIVGAGIF
                     FTPTLVARMTGDASLAMWTWVAGGLVAMLGALTFAELGGMYPDTAGQYEILRDAFAPW
                     VGFCFVVCNATAIVAAGTAIISIVCAENIAAIVGTLASPSRVTLTAVVVLAAAAGANM
                     VGVRWGAAIQNTTVMAKIVTLLLIAALAVFLAPASPPVAREAAGEAARGPAGLRLFAG
                     LVPTLFAYGGWQYALWVGGEVKNPRRNVPLAIVGGVAVVVVVYTVVNWAYLKLLGFDG
                     VAGSDALAADAVSVVWPGAGSRLIALAVALSAFGVLNVQILSGPRLLYGMARDGRFFS
                     VFGRTHPRFATPAIAIAFVAAISASLILAAGKGGIDKLLTGLVLIDAVFFGLTGLALI
                     VLRKKRPDADRPVRTPLYPIVPILFLMFECLIIYGAFRLGAAAAWIGVLWIAAASACY
                     ALFFRKRAAA"
     gene            complement(1389..1949)
                     /locus_tag="VEC56_11465"
     CDS             complement(1389..1949)
                     /locus_tag="VEC56_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXV14813.1"
                     /translation="MLRHPRRPLRWILFAALALAVGYAGVRFGRALRDRSAPPVADAP
                     PFPFRPGDPFPDVALSDSVDAIVRSDSLLAGRGAVVLFLDPNCEGCTDMSIRWEHALA
                     EGTIDPTRVFGVSRAGTGVNRSYRAAHRLSFPIYRDVEDAFLARYRVTSYPLEVVVGR
                     SGTIRSLSDDSVSPVDVESVRASMSE"
     gene            complement(2020..2226)
                     /locus_tag="VEC56_11470"
     CDS             complement(2020..2226)
                     /locus_tag="VEC56_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14814.1"
                     /translation="MKKLTAILALVGVFGVGALVGLRATTAQAGPCYYRCICSVAYKC
                     CTTETGTTCKAVPNAPINCPQVAC"
     gene            2395..3582
                     /locus_tag="VEC56_11475"
     CDS             2395..3582
                     /locus_tag="VEC56_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXV14815.1"
                     /translation="MPSNSSSNSSTGRANAILLVVTLVVLIVAAEITLRVVYHPEFLG
                     SVIHYDPVLGWSLEPNASLVSVDSQRGLRYRIDINSLGLRDRDLEIDKRRTARRVLIL
                     GDSFVFGVGVNAGERFSDVLGRALPDDVEVINAGVPGWGTDQEMLFYESSLRRLQPDV
                     VVLTFLGQNDVVNNGLRGPLIEVGTKPRFLCDGDRLLLEPPTPPAKLSFSQRAKRTLR
                     KSRLLLFIKRRFDMREYQHHAVEDPRFVTHGYEAHRHLSHWSVYDARGGEAIDEAWCV
                     TERVIDRLAADCREDSAELVVFAFPSQVEVDEPWREEMMKRTGVDPANMDFARPYRRL
                     SSFCAARGIEYHYPIDAFHAAAQKEPLYFDHDAHPNIAANALAAELLRDVVTLSLDHH
                     ARR"
     gene            complement(3579..4244)
                     /locus_tag="VEC56_11480"
     CDS             complement(3579..4244)
                     /locus_tag="VEC56_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14816.1"
                     /translation="MPKQKDLKRLVRSRMKKTGESYTTARTHVTRKSAPAKKPTDYAA
                     LAGMSDSAIQAKTGKTWAQWVRALDAIDATKLPHREIAEHVHTVFGVPGWWTQAVTVG
                     YERIKGLREIGQRRSGAYETSKSKTLPVPAKVAFEAFANARTRKKWLADIALTVRKAT
                     PSKSVRITWPDGTDVQVWITAKGEKCSVAVQHTKLASKEDVNARKAFWAERLAGLADL
                     LSD"
     gene            4866..6134
                     /locus_tag="VEC56_11485"
     CDS             4866..6134
                     /locus_tag="VEC56_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14817.1"
                     /translation="MLVRETHSNAILHTVDPRCVVSWKGAARRRASAAVVAAVLALAA
                     RDAGAQCAEQQFQNYTGTNQVACPCFAPNEQAGVVFTLPANQYPIEIVKVGIAWGSAI
                     GGTGQSIEEALHVYAGGLPNPGAPIFSLVGPVLNDGFFNEFNLDAFDITIASGPFMVA
                     LEFLNNNAGDIFAASVVHDGNGCQAGKNAVYSNGWFNACALGVTGDWVFYVKYRSLAV
                     TGGASPVRTLFSDPPLATTSCDTVYVVNNGCDDLVISSVTGCGSAPFSIDTTPTANTI
                     APGGSTPILVCVTPTGATTDSCLVSVTSNASNSPFTFRVVLDVTATDVDSPALDGFDI
                     VGVVPNPFNPSTSVRFTLPSALPVSAEVWSVTGAKVRTLADGATLSAGEHALRWDGRN
                     ANGERVASGVYMFRVSTPLGKRVARMVLVQ"
BASE COUNT          943 a         2056 c         2120 g         1137 t
ORIGIN      
        1 agcgcaccca caaactggtc ctcctcaagt agcgcgcacg atggccgtca ccgataccgg
       61 cctgcgacgc gttctcggtt ccgcgagcgc gaccgcggtg gtgatcgggg ccatcgtcgg
      121 cgccggcatc ttcttcacgc ccaccttggt cgcgcgcatg acgggcgacg cgtctctcgc
      181 catgtggacg tgggtcgcgg gcgggctggt cgcgatgctg ggggcgctca ccttcgccga
      241 gctgggcggg atgtaccccg acaccgcggg ccagtacgag atcctgcgcg acgcgttcgc
      301 gccctgggtc ggcttctgct tcgtggtgtg caacgccacc gccatcgtgg cggccggaac
      361 cgcgatcatc tcgattgtgt gcgcggagaa catcgccgcc atcgtgggca ctcttgcgag
      421 tccgtcgcgc gtgacactta ccgcggtcgt ggtgctcgcc gcggccgcgg gcgcgaacat
      481 ggtcggcgtg cgctggggtg ccgcgatcca gaacaccacc gtgatggcga agatcgtaac
      541 tctgctcttg atcgcggcgc tggcggtgtt cctggcgccg gcttcgccgc cggttgcgcg
      601 cgaggcggcc ggcgaagcgg cccgcggacc cgcgggattg cgtctcttcg ccggattggt
      661 gcccacactg ttcgcgtacg gcggctggca gtacgcgctg tgggtcggcg gcgaagtgaa
      721 gaatccgcgg cgcaacgtgc cgctcgccat cgtgggtggt gtggcggtcg tggtggtggt
      781 ctacaccgtc gtcaactggg cgtatctgaa gctgctcggc ttcgacggtg tggcgggaag
      841 cgacgcgctg gcggcggacg cggtctcggt ggtgtggccg ggcgcgggct cgcggctgat
      901 cgcgctcgcg gtggcactgt ccgcgttcgg cgtgctcaac gtgcagattc tctccgggcc
      961 gcgcctgctc tacggcatgg cgcgcgacgg ccgcttcttc tccgtgttcg gacgcacgca
     1021 cccgcgcttc gcgaccccgg ccatcgcgat cgcgttcgtc gccgcgatca gcgcctcgct
     1081 gatcctcgcg gcgggcaagg gcggcatcga caaactgctc accggattgg ttctgatcga
     1141 cgcggttttc ttcgggctca ccggtctggc gcttatagtt ctgcgcaaga agcgccccga
     1201 cgccgaccgg cccgtccgca cgccgctcta tcccatcgtg ccgattctgt tcctcatgtt
     1261 cgagtgcctg atcatctacg gcgcgtttcg gctgggagcg gcggcggcgt ggatcggtgt
     1321 cttgtggatt gcggccgcga gcgcgtgcta cgcgcttttc ttcagaaagc gcgccgcggc
     1381 ctagtcaact actccgacat ggacgcgcgc acggattcga cgtcgaccgg cgacacggag
     1441 tcgtcgctca acgagcggat cgtccccgat cggcccacca cgacttcgag cggatagctc
     1501 gtgacgcggt agcgggcgag gaacgcgtcc tcgacgtccc ggtagatggg aaagctcaag
     1561 cgatgggcgg cgcgatagct ccggttgacg ccggtgcccg cgcgactcac gccgaacacg
     1621 cgcgtggggt cgatggtgcc ctcggcgagc gcgtgctccc atcgaatcga catgtcggtg
     1681 cagccctcgc agttggggtc gaggaacagg acgacggcgc cccggcccgc gaggagcgaa
     1741 tcgctgcgca cgatcgcatc cactgagtcc gagagcgcga cgtcggggaa cggatcgccg
     1801 ggccggaagg ggaacggggg cgcgtcggcg accgggggag cggaacggtc gcgcagagcg
     1861 cggccgaagc gcacgccggc gtatccgacc gcgagtgcca gcgcggcgaa caggatccat
     1921 cgaagcggac gacgtggatg acgcagcatt ttttactccc ccggcgctcg acgcgagggc
     1981 ggacaagcca accgacccgc cgccgcgtcg acgacgcgcc tagcaggcga cttgcgggca
     2041 attgatcggc gcgttcggca ccgccttgca ggtcgtaccc gtctccgtgg tgcaacactt
     2101 gtacgcgacg ctacaaatgc agcggtagta gcacgggccc gcctgcgccg tcgtggctcg
     2161 caacccgacg agcgcgccga cgccgaacac tccgaccaac gccagaatcg cggtcaactt
     2221 tttcatgatt cacctcccct cggttggact tgggaggtta gtcccgctcc ggtgtggtgt
     2281 caacacggag gtcgcagcga ggcgtcgaag ctttcggttg tggcgtccct gtgctacaat
     2341 ggcgcccact ggcgctaggg gcggggctgc gctcgatgcg agcggtgcaa cgctatgccc
     2401 tcgaattcaa gttctaactc gtcgaccggt cgcgccaacg cgatcctgct cgtcgtcacg
     2461 ctggtcgtcc tcatcgtcgc ggccgagatc acccttcgtg tcgtttacca tcccgagttc
     2521 ctcgggagcg tcatccacta cgatcccgtg ctcggttgga gcctcgaacc caacgcgtcc
     2581 ctcgtgagcg tcgactccca gcgcggactg cgctaccgga tcgacatcaa ctcgctcgga
     2641 ctccgcgacc gcgatctcga gattgacaag cggcgcaccg cgcgtcgcgt cctgatcctc
     2701 ggagattcgt tcgtgttcgg cgtgggggtg aacgcgggcg agcgcttttc cgatgtgctc
     2761 ggtcgcgcgc ttcccgacga cgtggaagtg atcaacgcgg gtgtgcccgg gtggggcacc
     2821 gaccaggaga tgctgttcta cgagtcctcg ctgcgccgtt tgcagcccga cgtcgtcgtg
     2881 ctgacgttcc tgggacagaa cgacgtggtc aataacggtc tgcgcgggcc gctgatcgaa
     2941 gtcggcacca agccccgctt cttgtgcgat ggcgatcggt tactcctgga accccccacg
     3001 ccgccggcga agctgagctt ctcccagcgc gccaagcgca ccttgcgcaa gagccggctc
     3061 ttgctgttca tcaagcgacg cttcgacatg cgcgagtacc agcaccacgc ggtcgaggac
     3121 ccgcgcttcg tcacccacgg ctacgaggcg caccgccacc tttcgcactg gtcggtgtac
     3181 gacgcgcgcg gcggggaagc gatcgacgag gcgtggtgcg tgaccgagcg cgtgatcgac
     3241 cgcctggcgg cggactgtcg cgaggactcc gccgagctgg tggtgttcgc gttcccttcg
     3301 caggtcgaag tcgacgagcc gtggcgggag gagatgatga agcgcaccgg cgtcgacccc
     3361 gccaacatgg acttcgcccg gccgtatcgg cggctgtctt cgttctgcgc cgcacgcggc
     3421 atcgagtacc actacccgat cgacgcgttc cacgcggccg cgcagaagga gccgctctac
     3481 ttcgaccacg acgcgcaccc taatattgcc gccaacgcgc tggcggccga actgttgcgc
     3541 gacgtagtga cgttgtcgct cgaccaccac gcgcgacgtt agtcgctcaa gagatcggca
     3601 agccccgcga gcctttccgc ccagaacgcc ttgcgcgcgt tcacgtcctc cttcgacgcg
     3661 agcttggtgt gttgcaccgc gaccgagcat ttctcgccct tggcggtgat ccaaacctgg
     3721 acgtcggttc cgtcgggcca cgtgatgcgc accgacttgg acggcgtcgc cttgcgcacc
     3781 gtgagggcga tgtccgcgag ccacttcttg cgcgtgcgcg cattcgcaaa cgcctcgaac
     3841 gcgaccttcg ccgggacggg cagcgtcttg ctcttcgacg tttcatacgc gccgctgcgc
     3901 cgctgtccga tttcgcgcaa ccccttgatg cgctcgtagc cgaccgtcac cgcttgagtc
     3961 caccagccgg gaaccccgaa cacggtgtgg acgtgctcgg cgatctcgcg gtgggggagt
     4021 ttcgtcgcgt cgatggcgtc gagcgcgcgc acccattgcg cccaggtctt gccggttttg
     4081 gcttggatcg cgctatcgct catgcccgcg agcgcggcgt agtcggtcgg tttctttgca
     4141 ggagctgact tgcgggtgac gtgcgtgcgg gcggtggtgt acgactcgcc cgttttcttc
     4201 atgcgggagc gcacgaggcg cttgaggtct ttttgcttcg gcatgtcgat catcctttcg
     4261 atagccgtgc gcaacccgcc ccccagcagc gagcgtcgct cggcaacgaa agcgtcgacg
     4321 atgcgtgaat ccgggagtgt gccccccttt gccccacgaa ggccctcgct gggggaggac
     4381 cgaaggggtt gggcgtggat cgtcgcctgg tcgctattat ggaggaagca tcccacgggc
     4441 gacaaggggg agaatcatgc cgccccgtcg cgcaaaattc ttctttacga ccgggcgcgc
     4501 cttgcatagc ttttccgccg atggcatcgc gacgaaggtc gcgacggtct cggcggaccc
     4561 gggcggttcg aggggccggg cgaggtgggt ctcgccgatc attgggcacg ccctgaagga
     4621 ggtgatccag tgagtacaga tagtcgcggc ttagtggagg tggcgtcccc gtaaagggat
     4681 gactccacgt tcgtggcttt ctaaggtcac cccgcattcg ggggaaaccc aacgccacca
     4741 atactgatcc cggtcggggt ccctgcaagg ggaccccgac ttttttgccg gatcgcgctg
     4801 gcatgtcgct tgcggccgct cctcggggag ttctctagag ggcccaatgt gcccgaatga
     4861 ggtgcatgct cgtgcgcgaa acacactcga atgccatcct gcataccgtc gacccgcggt
     4921 gcgtcgtttc ttggaaggga gcggcgcggc ggcgcgcgtc cgcggccgtc gtggccgccg
     4981 tcttggccct ggccgcgcgc gacgccggcg cccagtgcgc cgagcagcag ttccagaact
     5041 acaccggcac gaaccaggtc gcgtgcccgt gcttcgcccc caacgaacag gcgggagtgg
     5101 tgttcacgct gcccgcgaac cagtatccca tcgagatcgt gaaggtcggt atcgcgtggg
     5161 gttcggctat cggcggaacc gggcagtcta tcgaggaagc gctccacgtc tacgcggggg
     5221 ggctgcccaa ccccggtgcg cccatcttct cgctggtggg gccggtgctc aacgacggat
     5281 tcttcaacga gttcaacctg gacgcgttcg acatcaccat cgcttcgggg ccgttcatgg
     5341 tggcgctcga gttcctcaat aacaatgcgg gcgacatctt cgcggccagt gtggtgcacg
     5401 acggcaacgg ctgccaggcg ggcaagaacg ccgtctactc caacggctgg ttcaacgcgt
     5461 gcgcgctggg tgtgaccggg gactgggtat tctacgtgaa gtaccgttcg ctcgcggtga
     5521 cgggcggcgc gagtccggtg cgcacccttt tctccgatcc gcccctggcc acgacttcgt
     5581 gcgacacggt ctacgtcgtc aacaacggct gtgatgacct cgtgatcagt agtgtgaccg
     5641 gttgcggcag cgcgcccttc agtatcgata ccacacccac ggccaacacc atcgcgccgg
     5701 gcgggagcac gcccatcctg gtgtgcgtga cgcccacggg tgcgacgacc gacagctgtc
     5761 tggtcagcgt gacgagcaac gcgagcaact cgcccttcac cttccgcgtc gtgctcgacg
     5821 tgacggcgac ggacgtcgat tcacccgcgc tggacggctt cgacatcgtg ggcgtggtgc
     5881 ccaatccatt caacccgtcg accagcgttc gcttcacgct cccgagcgcg cttccggtga
     5941 gcgccgaagt gtggtcggtg acgggtgcga aggtgcgcac gctcgcggac ggagcgacct
     6001 tgagcgcggg cgagcacgcg ctgcgctggg acggccgcaa cgcgaacggc gagcgcgttg
     6061 cgtcgggcgt ctacatgttc cgcgtgtcga cgccgctggg caagcgcgtc gcacgcatgg
     6121 tcttggtgca gtagtcttac ggaggtcgaa cgtgccgatg ttgaagtgga agcgaagcga
     6181 gttcgtcgtc accggtgaga tcatgacgcg gtccatgttc gactcgtggg ccgacgagga
     6241 cggtcgcgcg gcgttt
//
LOCUS       DASPGK010000416         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1387987, whole genome shotgun sequence.
ACCESSION   DASPGK010000416 DASPGK010000000
VERSION     DASPGK010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387987"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..948)
                     /gene="lgt"
                     /locus_tag="VEC56_11490"
     CDS             complement(145..948)
                     /gene="lgt"
                     /locus_tag="VEC56_11490"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXV14818.1"
                     /translation="MHPVLLELGRLQIRSYGFMLAVSFLLGIWLAGRRAKRHGIDPQK
                     ILDLSVVIILAAVVGSRLLYVAFHIDQYRNPLDIFALWQGGATFYGGFLLAVGASYWW
                     VQRNGLPFLTVADIVAPSIALGLVFTRVGCLLSGCCYGQPTEGPLGLVFPPDSPAGSA
                     AAALAQQMGRDHVALHPTQAYSSLKGLVIFSTLMLLQPKLTKPGSTFGLLLVLYGIGR
                     FTIDFFRFYEANARVFLGLSFNQAISIALIALGLVLLLRRAAPEKPADG"
     gene            complement(1013..2260)
                     /gene="rodA"
                     /locus_tag="VEC56_11495"
     CDS             complement(1013..2260)
                     /gene="rodA"
                     /locus_tag="VEC56_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXV14819.1"
                     /translation="MRVGRDFDWVIVLCSGLLSVIGLAVVYSVSRSVGEVSAVAMHGT
                     HFARQLVWLGFGLLTMSLAFVIPFRMFESLAFASYVACLIMLVVVLMLPARGETQRWF
                     IVGPLAIQPSEFTKVAVILVWARVLSTHRADPNRLRTLVPVLVLFLVPFALVLRQPDL
                     GTAMVFAGILLPVLFWRGFRGLHILFLLSPLVSSVLIIYGENVTHNPWPFGIFIVLIL
                     VVAYLRRAYLVESISLVVANVGIGLFLPALWNHLKPYQQRRVTNFLDPDSDKLGAGWQ
                     VIQSKIAIGSGGFDGKGYLEGTQKALEYLPAKHTDFIFSVLGEELGFVGAIGVLVLFS
                     ILITRALLIAQKTKSEFASTLCVGIAAYFAFQVFVNIGMTAGIAPVAGIPLPFVSYGG
                     SSMLVSCFLVGLLLNCAVRWSEY"
     gene            complement(2257..>3041)
                     /locus_tag="VEC56_11500"
     CDS             complement(2257..>3041)
                     /locus_tag="VEC56_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXV14820.1"
                     /translation="RFGNRVFKCWKEEGHGGMNLMSAIVNSCDIYFYRVGEMMDVDVL
                     ADAATAFGFDAKTGIDLPIEVSGNVPSRRYYDKRLGKGRWTQGLMLNNVIGQGEYLAT
                     VLHVARMSAAVANGGWLVTPHFVLPAEGAVPPARTRIPGLEGSTLTFLQRSMLAVVER
                     SDGTAHWTRIPWLEVAGKTGTAQNPHGDHHSWYTAYAPAGKPEIALAILVENAGHGGD
                     VSAPIARAFMAEYFRPGRRTEGAVSSAEQRDAPVPASTGGTP"
BASE COUNT          624 a         1019 c          973 g          425 t
ORIGIN      
        1 gatgccgccg cgtcggggcg cggcagatgc gcacccgagt cgccgcgccg gaggcaggcg
       61 tgacaccgtt catcgacgag gaaatcgacg agcgcttgga ccaccatccg caccccaccc
      121 atcaccaacg cctcccggcg cgcgtcatcc gtccgcgggt ttctccggcg cggctcgtcg
      181 cagcagcaag accaatccga gcgcgatcaa cgcgatgctg atcgcctggt tgaaggagag
      241 tccgaggaag acacgtgcat tggcctcgta gaagcggaag aaatcgatcg tgaagcgtcc
      301 tatgccgtac agcaccaaca agagcccgaa cgtcgacccg ggcttggtca gcttgggctg
      361 cagcagcatc aacgtcgaaa aaatcacgag gcccttgagc gacgagtagg cctgcgtcgg
      421 gtggagtgcg acgtgatcgc gtcccatctg ctgcgccaac gcggcggccg cgctgccggc
      481 cgggctgtcg ggcgggaaca cgaggcccag cggtccctcg gtgggctggc cgtagcagca
      541 cccgctcagc aggcacccga cgcgcgtgaa cacgagcccc agagcgatcg acggcgcgac
      601 gatgtcggcg acggtcaaga acggcagccc gtttcgctgc acccaccaat acgatgcgcc
      661 gaccgcgagc agaaagccgc cgtagaaggt cgccccaccc tgccacagag cgaagatgtc
      721 gagcggattg cggtactggt cgatatgaaa cgcgacgtac aggaggcgcg aacccacgac
      781 ggccgccagg atgatgacga cactcaagtc gaggatcttt tgggggtcga tgccgtggcg
      841 cttcgcgcgg cgcccggcga gccagatgcc gagcaggaag ctcaccgcca gcatgaagcc
      901 atacgagcga atttgcaggc gaccaagttc gagtaagacg ggatgcatgg gtttctctcc
      961 agccgggccc cggcaatata gcgcgttcgc gacgccgtgt cacgcgtccg cgctagtatt
     1021 ccgaccagcg caccgcgcag ttgagcaaga ggcccaccag gaagcagctc accagcatcg
     1081 aactgccccc gtagctcacg aagggcagcg ggatgccggc cacgggcgcg atcccggccg
     1141 tcatcccgat gttgacgaag acttgaaacg cgaaataggc cgcgatgccg acgcacaacg
     1201 tcgacgcgaa ctcgctcttc gtcttctgag cgatcaagag cgcccgcgta atgagaatgg
     1261 agaacaggac caagacgccg atcgcgccca cgaagcccag ctcctcgccc agcaccgaga
     1321 agatgaaatc ggtgtgcttg gcagggagat actcgagcgc cttctgcgtc ccctccaggt
     1381 aacccttccc gtcgaagccg cccgatccga tcgcgatctt cgattgaatg acctgccaac
     1441 ccgcgcccaa cttgtcggaa tcggggtcca gaaaattggt gacgcggcgc tgctggtagg
     1501 gcttgagatg attccagagc gcgggaagga agagtccgat tcccacgttg gcgaccacca
     1561 gcgaaatgct ttcaaccaga tacgcgcgcc gcaggtacgc aaccaccaag atcaggacga
     1621 tgaagatccc gaacggccac ggattatggg tgacgttttc accgtagatg atcaagacgc
     1681 tgctcaccag cggagacagg aggaacagaa tgtgcaaccc gcggaagccg cgccaaaaca
     1741 ggaccgggag cagaatgccc gcgaacacca tcgccgttcc caggtcgggt tgtcgcagca
     1801 cgagcgcaaa cgggacgagg aacagcacga ggacggggac gagggtgcgc agccgattgg
     1861 gatcggcgcg atgggtcgag agcacccgcg cccataccag gatcacggcc accttggtga
     1921 actcgctggg ctggatggca agcgggccca cgatgaacca gcgctgcgtc tctccgcgcg
     1981 ccggcagcat gaggacgacg accagcatga tcaggcaggc gacgtacgag gcgaacgcga
     2041 gcgattcgaa catgcggaac ggtatcacga acgcgagcga catcgtgagc aggccgaagc
     2101 cgagccacac cagttggcgc gcgaagtgcg tgccgtgcat ggcgaccgcg gagacttcgc
     2161 ccaccgaccg cgacaccgag tacacgacgg cgaggccgat caccgacagc agaccggagc
     2221 acagcacgat cacccagtcg aaatcccgcc cgaccctcac ggcgtccccc ccgtcgacgc
     2281 gggcacgggg gcgtcgcgtt gctccgcgct cgacaccgcg ccctcggtgc ggcgcccggg
     2341 ccggaagtac tcggccatga aggcgcgcgc gatgggcgcc gacacgtcgc cgccatgccc
     2401 cgcgttctcg accaggatgg cgagcgcgat ctccggcttc ccggcgggcg cgtaggccgt
     2461 gtaccacgaa tggtggtcgc cgtgcgggtt ctgcgccgtc cccgtcttcc ccgccacttc
     2521 gagccacggg atacgcgtcc agtgcgcggt gccgtcggat ctttccacca cagccagcat
     2581 cgagcgctgc agaaacgtaa gcgtcgatcc ctccagcccc gggatgcgcg tgcgcgcggg
     2641 cggcaccgcc ccctcggccg gcaaaacgaa gtggggagtc accagccacc ccccgttcgc
     2701 gaccgcggcc gacatgcgcg cgacgtgcaa cacggtcgcg aggtactcgc cttgaccgat
     2761 cacgttgttg agcatgagcc cctgcgtcca gcgccccttc cccagtcgct tgtcgtagta
     2821 gcggcggctc ggcacgttgc ccgagacctc gatcggcaga tcgatgccgg tcttcgcgtc
     2881 gaagccgaac gccgtcgccg cgtccgccag cacgtcgacg tccatcatct cgccgacgcg
     2941 atagaagtag atgtcgcagc tgttgacgat ggcggacatg aggttcatgc cgccgtgccc
     3001 ctcttccttc caacacttga agacgcgatt gccgaagcgg t
//
LOCUS       DASPGK010000417         8016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_453449, whole genome shotgun sequence.
ACCESSION   DASPGK010000417 DASPGK010000000
VERSION     DASPGK010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453449"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..589
                     /locus_tag="VEC56_11505"
     CDS             338..589
                     /locus_tag="VEC56_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14821.1"
                     /translation="MELLISLLSGGVGGNLFGALMKNRSMGPMLNTVLGALGGLAAGQ
                     LGAGALGGSMGGNIGVSAIGGILLPLIGSFFKKKSTPAS"
     gene            complement(660..3878)
                     /locus_tag="VEC56_11510"
     CDS             complement(660..3878)
                     /locus_tag="VEC56_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14822.1"
                     /translation="MPRRALRSATLAAVALVLSVVTGTDAAVDPGLEWKTISTEHFDV
                     HFHAGAEWTGAEAARIAEEVYGPITGLYGYQPKRCHIVILDTEDYANGAAYFYDNKIE
                     IWATNLEFHLRGTTPWLRNVITHEFTHIVSIQAAMKMPLRIPAIYFQAVDFESEKRPD
                     VITGYPDLIVSYPITGTVVPAWWAEGVAQYQSPSKQTDCWDTHRDMILRAAVVEDRML
                     SYDEMGFLGHRSLGNEQVYDHGYGLVRYIAATHGPESIAMTSEYLGHWNRLTIDGALE
                     KATGQSGDELYKGWKAYMRDRYDRMLADVRANPREGTILSDQGFMTLDPSFGPDGKRV
                     AVLSNKGSDFASTSLYLVDRDGKNFDRVEGGVSSRAVFSPDGALLLYAKHTRADVYGA
                     RLSDLYTYALATKKEKRLTHKLRASEPQWSPDGRSIVCVLNQDGTHRLVVLNADGTGL
                     REIRAGVSGVQLYSPQYSPDGSRILFGIFERGTRDVASVAPDGNDFRYELRTPNDERD
                     ARWTPDGAGIVFASDRTGIFNIYATNTETGEVAQLTNVVGGAFMPDLARDGTLAYSQY
                     TAKGYRAMTIASDAGTVATLTRDAYATRAAGPFDECTDLKLGGGFGVGDAFGIERVET
                     SIAPGIEGGTAPTETSTGTAQEGSTSLVPRAGGSGEAPSEPTVGVQAPALEPVPYKWD
                     YTGFQFFPRFLIWDGTPRLGLFVASNEILDRQSLFFGGSYGTDGEFDLIFDFELRRLF
                     PVIFMEYYKVRQKYEDQFPLEEFDRYYYVDYRYDVWSADLGLRFEFEDPYSLTQRNDI
                     QVWWNHSEYNIFLEPEYTPLNDPTAPRLPEQPVGWKYFVGNDARARWYYKSITRAIDS
                     DINPRGGREFTVEVMYAMDELFTSGEFEYGVNPDFDKNDFGQYTIDWREYLALPFGRH
                     TLQLRAFGSVIDRNVDDFFWVYMGGMDRLRGYTYYAIGGRKGALASATYRFPIWRRIN
                     KQASWLTFKDIYGGIFYEVASAWNSGNLPSDDPTLEKDYYSNVGVELRLNMGSFYSYP
                     TTVNVAGAYALEEARYVNPVFDVPVVEYDPQWRVYLNLGFGF"
     gene            4075..5415
                     /gene="rmuC"
                     /locus_tag="VEC56_11515"
     CDS             4075..5415
                     /gene="rmuC"
                     /locus_tag="VEC56_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXV14823.1"
                     /translation="MSTGITVALALGVVVGFSAAWLIARSRAAALRAQNEGKDRALAA
                     RDEAVVDLKVQVARLESTLEQERASETRLSNAFKALSADALKSSNESFLELAKATLEK
                     YQAGAKNDLEQRAKSVEEFVKPLKESLEKVNVQIETIEKTRREDYGSLSQHLMGLRTE
                     TSSLNTETRNLTNALRTPIVRGRWGEVQLRKVVELAGMVEYCDFVEQPSVEGEEGRLR
                     PDLVVNLPGGKNVVVDSKVPLEAYLNAQESTDVETARAYMKDHARQIRVHIGKLRAKS
                     YAEQFDSTPEFVVMFLPGESFFSAALEHEPGLIEEGAAQNVIIATPTTLIALLRSVAY
                     GWKQEKIAESAQEIRDLGIELHKRMRVLAEHVMDIGDGLNRAVGAYNKSVGSLESSVL
                     PAARRFPELGLTGQPEIPQLTPVERTTRALNSPELSLFTLHEKPNVIDAADAHP"
     gene            complement(5416..6168)
                     /locus_tag="VEC56_11520"
     CDS             complement(5416..6168)
                     /locus_tag="VEC56_11520"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV14824.1"
                     /translation="MTLEGKVAIVTGASSGIGKSIAQLYAREGAAVVLSDLNGDSGER
                     VAAELRDAGARTIFVKANVAEPADSERTVKAALETFGRLDCACNNAGIGGEQSPTADI
                     SLEGWQKVIGINLSGVFYGMKYQIPAMLKSGGGAIVNIASILGQVGFANSSGYVAAKH
                     GVVGLSKNAAIEYAAKNVRVNSVGPGFIHTPLIGALERDRHVNEMLISLHPMGRLGNP
                     EEVAELVLWLSSDKSSFVTGGYYAVDGGYLAR"
     gene            complement(6165..7088)
                     /locus_tag="VEC56_11525"
     CDS             complement(6165..7088)
                     /locus_tag="VEC56_11525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXV14825.1"
                     /translation="MASINLNHLHYFYEVARHGSFTRAARELLVSQSALSVQIRALEN
                     SLGSRLFDRRSGGVVLTDAGQRAFAVAERVFADVDQLISDFRQSDRLLSGAVSVGTVN
                     SIGIYVLPDILTAYRAKYPDVRIHIDFRESERVMDQLMQGKTDLAVVPWERSYPDLNA
                     MPLTRHKLFIVAPPDHPLARAELTNPRDLEGYPFVGYHEGMHTRAMIDALFKRMSVTV
                     QYAVESTNAATIKHMVMAGMGLGFLPEAAVAPEIRRGQLVRLEIPSLVMSQDVTLYLR
                     KNRTLPRAAVGFVEFLKEYFNPKKRGREGTQ"
     gene            7713..>8016
                     /locus_tag="VEC56_11530"
     CDS             7713..>8016
                     /locus_tag="VEC56_11530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HXV14826.1"
                     /translation="MVFVPSGEFLMGSTSDEVLKQADLDEFPQRRVFVDDFYIDVHEV
                     TNAQYKVFLDSTRVEAPPRWLNGDYGIGEDGFPVISITWDEAVAYATWLGKRLPTEA"
BASE COUNT         1452 a         2563 c         2542 g         1459 t
ORIGIN      
        1 tgacgatgac accccaagca tgtaaaccgc tggatacgat gcggggagcg ttgacgcagg
       61 aagaagccct tgcgcttgaa cggccacgcg ggcgacaacg tgttgaagta tgtgcagttg
      121 gcgttgggac aatgtggagg cacccatccc gtcaatcccg gtcccatgtc tcatccccgg
      181 cacatcccgt gccagggaca ctctcgcggc cacaacctga acaatttgct tgccagtcgg
      241 tgcgggccct ccgacactat ggctcggccc aacgcaatcg acaccgtcgc tcaggcaacg
      301 gcctcaacgt tcgaatgccc tctcttaagg aggactcatg gaactcttaa tcagcctact
      361 gagcggcggc gtgggcggca atctcttcgg cgcgctcatg aagaaccgca gcatgggccc
      421 gatgttgaac acggttctgg gagcgctcgg cggtctcgcg gctggtcaac tcggcgccgg
      481 cgcgctcggc ggatccatgg gcggcaacat cggtgtgtcc gcgatcggcg gcattctgct
      541 ccccttgatc gggagcttct tcaagaagaa gtcgacgccg gcctcgtagc gggcgcgatc
      601 gtacccatcg aagacggccc gcagcgatcg tgcaccgcgg cgggccgttt ccttgtgagc
      661 tagaatccga atccgagatt gagatagacg cgccactgtg ggtcgtactc gaccacgggc
      721 acgtcgaaca ccggattgac gtagcgcgct tcctcgaggg cgtatgcgcc cgccacgttg
      781 accgtggtcg gatacgaata gaacgaaccc atgttcaagc gcaactcgac gccgacgttg
      841 gagtagtagt ccttctccag cgtgggatcg tccgacggca agttgccgct gttccacgcg
      901 ctcgccactt cgtagaagat gccgccgtag atgtccttga aggtcagcca agacgcttgt
      961 ttgttgatgc gccgccagat tgggaatcga taggtggcgc tggcgagcgc acccttgcgc
     1021 ccaccgatcg cgtagtaggt gtagccgcgc aggcggtcca tgccgcccat gtaaacccaa
     1081 aaaaagtcgt cgacgttccg gtcgatgacg gacccgaacg cgcgcaactg gagtgtgtgg
     1141 cgtccgaacg gcagcgccaa gtactcgcgc caatcgatcg tgtactgtcc gaaatcgttc
     1201 ttgtcgaagt cgggattgac gccgtactcg aactcgccgc tggtgaagag ctcgtccatc
     1261 gcgtacatca cttcgactgt gaactcccgg ccaccgcgcg ggttgatgtc gctgtcgatg
     1321 gcacgtgtga tcgacttgta ataccagcgc gccctcgcgt cgtttccgac gaagtacttc
     1381 cagcccaccg gctgctccgg caaccgcggc gcggtcgggt cgttgagcgg ggtgtactcg
     1441 ggctcgagga agatgttgta ctcggaatgg ttccaccaca cctggatgtc gttgcgctgc
     1501 gtcaggctgt agggatcctc gaactcgaag cgaagcccca agtccgccga ccacacgtcg
     1561 tagcgatagt caacgtagta gtagcgatcg aactcctcca gcggaaactg atcttcgtac
     1621 ttttgccgca ccttgtagta ctccatgaag atcacgggaa acagccggcg cagctcgaag
     1681 tcgaagatca agtcgaactc gccgtcggtt ccgtaggaac cgccgaagaa gaggctctgg
     1741 cggtcgagga tctcgttgct cgcgacaaag agaccgaggc gcggcgtccc gtcccaaatc
     1801 aggaagcgcg ggaaaaactg gaagccggtg taatcccact tgtacggcac cggctccaac
     1861 gcgggcgctt gcacgcccac cgtgggctcg ctgggcgcct cgccgctccc gcccgcgcgc
     1921 ggcaccagcg acgtgcttcc ctcctgcgcg gtgcccgtcg acgtctcggt cggggcggtg
     1981 ccgccctcga tgcccggcgc gatcgacgtc tccacgcgct cgatcccgaa cgcgtcgccg
     2041 acgccaaacc cgccgcccag cttgaggtcg gtgcattcgt cgaacggtcc ggccgctcgc
     2101 gtcgcgtacg cgtcgcgcgt gagtgtcgcc accgtgcccg catccgaagc gatcgtcatc
     2161 gcgcggtagc ccttcgcggt gtattgcgaa tacgccaggg tcccatcgcg cgcgaggtca
     2221 ggcatgaatg cgccgcccac gacgttggtc agctgcgcca cttcgccggt ctccgtgttc
     2281 gttgcgtaga tgttgaaaat accggtgcgg tcggaggcga acacgatgcc ggcgccgtcc
     2341 ggcgtccagc gcgcgtcgcg ttcgtcgttg ggcgtgcgca gctcgtagcg gaagtcgttg
     2401 ccgtcggggg cgaccgaggc cacgtcgcgc gtcccgcgct cgaagatgcc gaacaggatg
     2461 cggctcccat cgggcgagta ctggggcgaa tagagctgga cgccgctaac gccggcccga
     2521 atctcgcgca ggccggttcc gtccgcgttc aggaccacca aacggtgcgt gccatcctgg
     2581 ttgagcacgc acacgatgga gcgcccgtcc ggcgaccact gcggctccga ggcgcgcagc
     2641 ttgtgggtga gacgcttctc tttcttggtc gcgagcgcgt aggtgtacag atcgctcaag
     2701 cgcgcgccgt acacgtccgc gcgcgtgtgc ttcgcgtaca ggagtaacgc gccgtcggga
     2761 gaaaacaccg cgcgcgacga cacaccgcct tcgacacggt cgaagttctt gccgtcgcga
     2821 tcgaccaggt agagcgaggt ggatgcgaag tcgcttccct tgttcgaaag cacggcgacg
     2881 cgtttcccgt ccggtccgaa cgacgggtcg agggtcatga aaccctggtc ggagaggatc
     2941 gttccttcgc gcggattcgc gcgcacgtcg gccagcatgc gatcgtagcg gtcgcgcatg
     3001 taggctttcc aacccttgta gagctcgtcg ccgctctggc ccgtcgcttt ctcgagcgcg
     3061 ccgtcgatgg tgagacggtt ccaatggccg aggtattcgc tggtcatggc gatgctctcg
     3121 ggcccgtggg tggccgcgat gtagcgcacg agcccgtagc cgtggtcgta cacctgctcg
     3181 ttgcccaggc tccggtgccc gaggaacccc atttcgtcgt aggagagcat gcggtcctcg
     3241 accaccgcgg cgcgcaggat catgtcgcgg tgcgtgtccc agcagtcggt ctgcttgctc
     3301 ggcgactggt actgcgcgac accttccgcc caccacgccg gcaccaccgt gcccgtgatg
     3361 ggatacgaga cgatcaaatc cgggtagccg gtgatgacgt cggggcgttt ttcgctttcg
     3421 aagtcgacgg cctggaagta gatcgcgggg atgcgcaggg gcatcttcat cgcggcctgg
     3481 atcgagacga tgtgtgtgaa ctcgtgcgtg atcacgttgc gcagccacgg cgtggtgccg
     3541 cgcaggtgga actccaggtt cgtggcccag atttcgatct tgttgtcgta gaagtaggcg
     3601 gcgccgttgg cgtagtcctc ggtgtcgagg atcacgatgt ggcagcgctt gggctggtac
     3661 ccgtagaggc ccgtgatcgg cccgtagacc tcctcggcga tgcgggccgc ctcggcgccg
     3721 gtccactccg cgccggcgtg gaaatggacg tcgaagtgct cggtggagat ggtcttccac
     3781 tcgagccccg ggtcgacggc cgcgtcggtg ccggtgacga cggacaggac cagggcgacc
     3841 gcggcgagtg tggcgctgcg cagggcacga cgtggcaaga gcatggacac cgctggagtc
     3901 tacaagggga gggttaacgg tccgtggaac gttttcgtag cgaacaattt agcgcgttcg
     3961 cgagggccct gtcaattctc gtttttttct ttccgcgcgc gggtacgtcc gtcgttaaat
     4021 actaatgacg aatggggccg ccctccctac actggcggtc atgaaaggaa ttcgatgagc
     4081 accgggatca ccgtcgcgct cgccctcggg gtcgtcgtgg gattctcggc cgcctggctg
     4141 attgcccgct cgcgggcggc cgcgctgcgc gcgcagaacg agggaaagga tcgtgccctc
     4201 gcggcgcgcg acgaggccgt cgtcgacttg aaggtccagg tcgcgcggct cgagtcgacg
     4261 ctcgagcagg agcgcgcctc cgagacgcgc ctgtccaacg cgttcaaggc cctctcggcc
     4321 gacgcgctca agagcagcaa cgagtctttc ctcgagctcg cgaaggccac gctggagaaa
     4381 taccaggccg gcgccaagaa cgacctcgag cagcgcgcga aatcggtgga ggaattcgtg
     4441 aagccgctca aggagtccct cgagaaggtc aacgtacaga tcgagaccat cgagaagaca
     4501 cgacgcgaag actacggctc gttgtcgcag cacctcatgg gtctgcgcac ggagaccagt
     4561 tcgctcaaca ccgagacgcg caacctcacc aacgcgctgc ggacacccat cgtccgtggt
     4621 cgttggggcg aggttcaact gcgcaaggtg gtcgaattgg ccgggatggt ggagtactgc
     4681 gattttgtcg agcagccgtc ggtcgaaggc gaggaaggcc gattgcgccc cgacttggtg
     4741 gtcaatcttc ccggcggcaa gaatgtcgtc gtcgattcga aggttccgct cgaagcctac
     4801 ttgaacgccc aggaatccac cgacgtcgag accgcgcgcg cctacatgaa ggaccacgcg
     4861 cggcagatcc gcgtccacat cggcaaattg cgcgcgaagt cgtatgcgga gcagttcgat
     4921 tcgactccgg agttcgtcgt catgttcctc ccgggcgaaa gtttcttctc cgcggcgctc
     4981 gagcacgagc ccgggctcat cgaagaagga gcggcgcaga acgtcatcat cgccacacca
     5041 accacactca tcgcgctcct gcgctcggtg gcgtacggat ggaagcaaga gaagatagcg
     5101 gaaagtgcgc aggagattcg cgacctgggg atcgaactgc ataaacgtat gcgcgtgctt
     5161 gcggagcacg tcatggatat cggggacggg ctcaaccgag cagtgggtgc atacaacaag
     5221 tcggtaggat cgctggagtc gagcgtgctt ccggccgcgc gccggtttcc agagttgggc
     5281 ctaaccggcc aacccgagat accccaactg acccccgtgg agcgaacgac gcgagcgttg
     5341 aactcgcccg aattgtccct cttcacgctg cacgagaagc cgaacgtgat cgatgccgcc
     5401 gacgcacacc cgtagctaac gcgccaggta gccgccgtcg accgcgtagt acccgccggt
     5461 cacgaacgac gacttatccg agctcagcca cagcaccagc tcggcgactt cctcgggatt
     5521 ccccaagcgc cccatggggt ggagcgagat cagcatctcg ttcacgtggc ggtccctctc
     5581 cagcgccccg atcagaggcg tgtggatgaa tcccggtccc accgaattca cgcggacgtt
     5641 ctttgcggcg tactcgatcg ccgcattctt cgacaggccc acgaccccgt gcttggccgc
     5701 cacgtagccg ctcgagttcg cgaagccgac ctgtcccagg atcgacgcga tgttgacgat
     5761 cgcaccgcct ccgctcttga gcatcgcagg aatctgatac ttcatgccgt agaacacgcc
     5821 cgaaagattg atgccgatca ccttctgcca gccttcgagc gagatgtccg cggtcgggct
     5881 ctgctcgccg ccgatacccg cgttgttgca cgcgcaatcc aggcgcccga acgtctcgag
     5941 cgctgccttc accgttcgct cgctgtccgc cggctcggcc acgttggcct tgacgaagat
     6001 cgtccgggcg ccggcgtcgc gcaactcggc cgcgacccgt tcgccgctgt cgccgttgag
     6061 atccgacaac acgaccgcgg ccccctcgcg cgcgtagagc tgcgcgatcg acttgccgat
     6121 cccggacgac gcgccggtga cgatcgcgac tttgccttcg agtgtcattg cgttccctcc
     6181 cgtccccgct ttttcgggtt gaagtattct ttcagaaact cgacgaaacc gaccgcggcg
     6241 cgcggcagcg tccggttctt gcgcaggtag agcgtcacgt cctggctcat gacgagggac
     6301 gggatctcga ggcgcaccag ttgcccgcgc cggatctccg gcgccaccgc ggcctcgggc
     6361 aagaacccga gccccatccc ggccatgacc atgtgtttga tggtcgccgc gttggtcgat
     6421 tctaccgcgt attggaccgt aaccgacata cgcttgaaga gcgcatcgat catggcacgc
     6481 gtgtgcatgc cctcgtggta gccgacgaac gggtaccctt cgaggtcgcg cgggttggtc
     6541 agttccgcgc gcgcgagcgg gtggtcgggg ggcgccacga tgaacagctt gtgccgggtc
     6601 agcggcatcg cgttcagatc cggataggaa cgctcccacg ggaccaccgc gaggtcggtc
     6661 ttgccttgca tgagctggtc catgacgcgc tccgattcgc gaaagtcgat gtggatgcgc
     6721 acgtcgggat acttcgcgcg gtaggcggtg aggatgtcgg ggagcacgta gatgccgatg
     6781 ctgttgacgg tgccgacgct gaccgcaccg gagagcaagc gatcgctctg gcgaaagtcg
     6841 gagatcaact ggtcgacgtc ggcgaacacg cgctcggcca ccgcgaacgc gcgctgcccg
     6901 gcgtccgtca gcaccacccc gccgctgcgc cggtcgaaca ggcgcgagcc cagcgagttc
     6961 tccagagctc ggatctgcac gctcaacgcc gactgcgaaa ccaagagctc gcgggcggca
     7021 cgcgtgaagc tcccgtggcg cgcaacctca tagaagtagt gcaggtggtt cagattgata
     7081 gatgccatat cttttactta tgtcaccaat atacattttc tattttacga atatcaaggt
     7141 cgaatctata ttcctctgcg aagccggtac cgcgggaaga acatgcggca accgttcgga
     7201 tgatagatgg gttgaggcga ccggtatcgg cttcccagaa tttatccgca aagcagcagg
     7261 aagaggaagc ggcgcgaacc tagtcccaga gtcccagaac gcgcctttct tcagtcccca
     7321 ccagaggaag gcagtctgtc gcaagacggg ctgccttcca ccacattcaa ctcgacccat
     7381 atgaagtccg accacgggct cctggcaacg ggagcccgta ttttttgccg cccgctcggg
     7441 cgagtagtct tgcgggccca tccgactcca taacaccaga ataggattca cgtttactcg
     7501 ctcccgcggc ccggcgtaga gttgccctcc ggcatcctct cccccgccgg aggtcgtcgc
     7561 ccaaagctcc aactatgcga tctgtcttcg tccttgtcgc cgtcccggcc gttgtgatcg
     7621 ctgcgctcgc cctcgccggg caagcgcagg acgttccgtc gcgccccggc gaagcgccct
     7681 tccggcccaa tcccgtccca gaggtcaacg gaatggtgtt cgtaccatcg ggtgaattcc
     7741 tcatgggtag cacgtcggac gaggtgctca agcaggccga cctcgacgaa ttcccgcagc
     7801 ggcgcgtgtt cgtggacgac ttctacatcg acgtccacga agtcaccaac gcgcagtaca
     7861 aggtcttcct cgattcgacc cgggtcgaag caccgccgcg gtggctcaac ggggactacg
     7921 gcatcggcga ggacggattc ccggtgatca gcatcacctg ggatgaagcg gtcgcgtacg
     7981 ccacctggct cggcaagcgc ctgccgacgg aggcgg
//
LOCUS       DASPGK010000418         9387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1624620, whole genome shotgun sequence.
ACCESSION   DASPGK010000418 DASPGK010000000
VERSION     DASPGK010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624620"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VEC56_11535"
     CDS             <1..834
                     /locus_tag="VEC56_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV14827.1"
                     /translation="LALLDAGDEVIYPNPGFPIYESMIDYIGAKRVPLMLSEKNDFSV
                     DPDHLASLVTKRTKLLILNSPHNPTGGVLTREAVARLSELAVKHDFYVLSDEIYARIQ
                     YEGEPVSPYPFPGMRERTIVLDGHSKTYAMTGWRLGYGVMPKEMALKVAKLQTNSNSC
                     TCSFTQIAGMEALHGPQDSVVAMVKEFRARRDLMVEGLNKVRGFRCHRPAGAFYVFPN
                     ITGTGKDSKEMENYLMEEAGVAVLAGTAFGSNGEGFIRLSYANSQANIKRALEKIAAA
                     F"
     gene            1042..2301
                     /gene="purD"
                     /locus_tag="VEC56_11540"
     CDS             1042..2301
                     /gene="purD"
                     /locus_tag="VEC56_11540"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXV14828.1"
                     /translation="MKVLVVGSGGREHALAWAIGRSPRVDRMWIAPGNAGTAGAGENV
                     DIGATDVAALVDFAREQRIDLTVVGPEAPLLAGIVDRFRASGLSCYGPTAAAARLEGS
                     KAFAKDFMRRRGIPTAAFATFEDAARARSYARSLGAPVVVKADGLAAGKGVVVAADVD
                     EAERAIDAMLVEQRFGEAGSRVVVEEFLQGEEVSVHAVCAGGHALLLPSSQDHKRAYD
                     GDRGPNTGGMGAIAPVPWVDAVALARVEREVVRPVLDGMAAEGTPFTGTLYAGIMWTE
                     AGPKVLEFNARFGDPETQSLMPLLVEGDVCGLLASAANGNLPEGVKAAARSAATIVVA
                     SKGYPESAQSGVEIRGHDAAEEGTMVFHAGTRVVDGRVVTAGGRVLGITGWAPTLSEA
                     LGRAYRVAARIHIDGAFYRSDIGRRHL"
     gene            2314..2781
                     /gene="purE"
                     /locus_tag="VEC56_11545"
     CDS             2314..2781
                     /gene="purE"
                     /locus_tag="VEC56_11545"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXV14829.1"
                     /translation="MAKAPRVAILLGSKSDEGHLKATTDLLDRFQIDWELKVISAHRQ
                     PEQLHEYVRAADAGGTLVFIAAAGLSAALPGVIGSISQKPVVGLPIPGGALAGVDALL
                     SMVQMPGGIPVATVGVGSNGSKNAAILAAQIIALGDPKVAERLRAYREELRTG"
     gene            2778..3404
                     /locus_tag="VEC56_11550"
     CDS             2778..3404
                     /locus_tag="VEC56_11550"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXV14830.1"
                     /translation="MTPRASLAGFIDDPRAQVLAAESLVRGAVVVLPTDTLYGLSAAL
                     SHEEAVRRIASIKQAPQERRFIVLASSIEMVERYVASFGCATREDLAARWPAPFTAIL
                     PSGRACPAWVGPTVAVRVPAFDPIRALIERVGEPIVSTSVNRTGREPLDDAPAIMREF
                     GDEIDAVFERRGEGGGASTIVDLCGKTARVVRAGGYAWDATGGGKPSK"
     gene            complement(3362..4228)
                     /locus_tag="VEC56_11555"
     CDS             complement(3362..4228)
                     /locus_tag="VEC56_11555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HXV14831.1"
                     /translation="MIQLAHPKVVQIETTLACNAECPFCSHSTLTRRPRRMEDWLWKK
                     IIDETRGLGITYRPFLINEPLADHRLGEIMRYIRKDDTAKIEINSNGELMKEERAQEI
                     LDVGIDIIRFSIDGFSKETFSKSRVGLDYDLTVERVRRFVEMARERGSAGHVEVRMIA
                     MDYNRHEHEPFVEFWTKVGAVAVITDLYVWPWEPEVKPVQLPCKKVLKEMFFFVNGKA
                     TLCCWDSHERGVIGDATRENVIDIWNGEVNRRYRDLLAQGRRREILLCSNCEAYKGHY
                     FEGFPPPVASHA"
     gene            complement(4251..5492)
                     /locus_tag="VEC56_11560"
     CDS             complement(4251..5492)
                     /locus_tag="VEC56_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HXV14832.1"
                     /translation="MSGARWVYNALLPAAVLGAHAAARLLPKMRAAVDGRRGFRGRWE
                     ALARDLNAPPVWFHVASVGEFEQARPVISALERARPDLPVVLTFSSPSGYAFARKRET
                     PGRGSLRFIDYLPFDTAPNMRFCLEQVKPRLLVFVKFDLWPNLVWEADARGVPIVLID
                     ATLSASSGRLAPPARWLYRDVYRRLDAILAISDDDAARFRATVPSHPSVSVTGDTRFD
                     RVMDRWQQRTASTFALPAGGPTLIAGSTWPPDEERLLPALARLLSEDARLRVVLVPHE
                     PTPAHVEPLRRWAAGTGSSARIVIVDVVGVLAEAYRFADLVYVGGAFTTGVHSVIEPA
                     IAGVPVVFGPKHDNSFEALQLVERGAARVIRTADEAYHALAAWLRDEAARKSAGAAGR
                     AYVESQLGATEKCMAALAPYL"
     gene            complement(5492..6505)
                     /locus_tag="VEC56_11565"
     CDS             complement(5492..6505)
                     /locus_tag="VEC56_11565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV14833.1"
                     /translation="MRVLVIRFSSLGDCVLLCPLLAHLKRAGAAEVVVLTKRAYADVF
                     AHAEGVDRVLAIEPGTSLLGLARIGRSFRQGARPGDVVIDAHSNPRSRIVSAAAGGAH
                     ARFRKYYRERAGLIVFKRPASLPPILAQYAALAEQAGFGATTLLPGGLRIPRAESETA
                     SLRVGGDQPAVAVAPGSRWRAKRWGGFAALCDGLVAAGRRVVLVGDARDREFTAPIAA
                     SLGSHCVDAAGQTTLIQTAALIARCESFVGNDSGLMHLAEAVGVPVTALFGPTVREFG
                     YAPSLLPSTTIERRLACRPCSRNGAAPCPKRTYECLDGIRVDDVLHTVLRPGPGERHR
                     VVD"
     gene            6572..7120
                     /locus_tag="VEC56_11570"
     CDS             6572..7120
                     /locus_tag="VEC56_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXV14834.1"
                     /translation="MPTHSFELGERIKKLRLSQNLTLKQLEAKAGVSATHLSEIERGQ
                     SSPTVGTLERVARALGEEPALLAGERTPRRVAVVRRNERRAWAAGEARVASLGTPLDG
                     AEMSVVEIELGTGETGFDPGPVANEEFLLVLAGAIDLRVGSTSHALAAGDAIHLATNE
                     SRRLHAREAARVLWITLPASTL"
     gene            7139..7702
                     /locus_tag="VEC56_11575"
     CDS             7139..7702
                     /locus_tag="VEC56_11575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXV14835.1"
                     /translation="MDVGSRVKRVRHEKGLTLKAVESLSGVSATHLSEIERGETSPTL
                     GSLARIARALSKPTAYFLEDNELGPVSRVGAKDRVREAVKGSARRGTASIERLTAGVP
                     GGRLHARRVELPPGSEYRAERHAHAGFEAMLVLDGRVRVDAGEQSFDLAEGDAIHFDA
                     SLPHAYANASRDEAAALIWVATRRDVD"
     gene            7829..8170
                     /locus_tag="VEC56_11580"
     CDS             7829..8170
                     /locus_tag="VEC56_11580"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXV14836.1"
                     /translation="MKPDVSRDELLFLHLVSMLQFAAMQQMGKVPNPVSGEIERDLEH
                     ARASIDMVEMLHAKTSGQRTAAESEFIDKVLFELRMNFVDETKRVEKERAGTEGMAPD
                     APGAGGTDAGR"
     gene            8245..>9387
                     /locus_tag="VEC56_11585"
     CDS             8245..>9387
                     /locus_tag="VEC56_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXV14837.1"
                     /translation="MQGLPRSGRMGLFLVLAALFALPEGATASASYNGTRGLIRTKSA
                     DTYRKGSLSFQLSSNYGKQDNEQLSSGLFFPLGDSATVDYHTFITRATITYSLSDFFE
                     IAGDLEVRNWIRTIQEQSPGASEDTYTRGGLGDTDVSAKLNIPLPSKKFKLGAYGNMV
                     FPTGDKNKGFSIDANDITILGLATIDMSDLQSFVPTRLHINAGYRLNKGEVPTDSLGY
                     GIFDPNFPDISGFNPPGYPPVPTGESDSYNDAFLFNAALEFPAPSVTFFVEFDMNQLL
                     NVDLPANAGVNASTYTLTPGVEFKGKGSSLELGADINLNSGDNPSLVNPPDWGLWIAF
                     NYVAEVVPRDSDKDGIADNVDGCPDEAEDIDGKDDKDGCPDLDNDGD"
BASE COUNT         1557 a         3130 c         3250 g         1450 t
ORIGIN      
        1 ctggcgctcc tcgacgcggg cgacgaggtc atttatccga accccgggtt tcccatctac
       61 gagagcatga tcgactacat cggtgccaag cgcgtgccgc tcatgctctc ggagaagaac
      121 gacttctcgg tcgatcccga ccacctcgcg tcgttggtga cgaagcgcac aaagctgctc
      181 attctcaatt cgccgcacaa cccgaccggc ggcgtgttga cccgcgaggc ggtcgcgcgt
      241 ctctccgagc tcgcggtcaa acacgacttc tacgtcctct cggacgagat ctacgcgcgc
      301 atccagtacg aaggcgaacc ggtgagcccg tacccgtttc cgggcatgcg cgagcgcacc
      361 atcgtcttgg acggccactc caagacctac gccatgacgg gctggcgcct gggctacggc
      421 gtcatgccga aggagatggc cctcaaggtc gccaagctcc agaccaattc gaattcgtgc
      481 acgtgcagct tcacccagat cgcgggcatg gaagcgctgc acggtccgca ggacagcgtg
      541 gtcgcgatgg tcaaggagtt ccgcgcgcgc cgcgacttga tggtcgaggg cttgaacaag
      601 gtcagaggat tccgctgcca tcgccccgcg ggcgcgttct acgtcttccc gaacatcacc
      661 ggcaccggca aggactccaa ggagatggag aactatctca tggaggaggc cggcgtcgcc
      721 gtgctggccg gcaccgcctt tggctccaac ggcgagggct tcatccggct ctcgtacgcg
      781 aactcacaag ccaacatcaa gagagcactc gagaagatcg ccgccgcgtt ctgacccgtt
      841 gaggcgagca ccgagggctt ctcccactac tccgaacgtc acccggcggg tgtcgctcgt
      901 ggggcccccg ccgcgccagg cggcggggtg acgagtgaca ggacccgccg ggcagcgttc
      961 tcgtcgccaa cttgacatcg atcgcaagca ctcggtatcg tcggcgtgtt ccacccgtca
     1021 ccctttcccc gggagtctcg tttgaaggta ttggtggtcg gttcgggcgg gcgcgagcac
     1081 gcgctcgcgt gggcgatcgg ccgctcgccg cgcgtcgatc gaatgtggat cgcgcccggc
     1141 aacgcgggca cggcgggcgc gggtgagaac gtcgacatcg gcgccaccga cgtggcggca
     1201 ctcgtcgact ttgcgcgcga gcagcgcatc gatctcacgg tggtgggacc ggaggcgccc
     1261 ctcctggcgg ggatcgtcga ccggttccgg gcctccgggc tgtcgtgcta cgggcccacc
     1321 gcggcggccg cgcgcctgga gggcagcaag gccttcgcga aggacttcat gcggcgccgg
     1381 ggtatcccca ccgccgcctt cgcgacgttc gaggatgcag cgcgcgcgcg ctcgtacgcg
     1441 cgctcgctcg gcgcgcccgt ggtcgtgaag gcggacgggc tcgcggccgg caagggtgtg
     1501 gtggtggccg ccgacgtcga tgaagccgag cgtgccatcg acgcgatgct ggtcgagcag
     1561 cgcttcggcg aagcgggctc gcgcgtcgtg gtggaagaat tcctgcaagg cgaggaagtc
     1621 tcggttcacg ccgtgtgcgc gggcggacac gcgctcctct tgcccagctc gcaggaccac
     1681 aagcgcgctt acgacggcga tcggggtccc aacacgggcg ggatgggggc gatcgcgccc
     1741 gtaccgtggg tggacgccgt ggcgctggca cgcgtcgaac gcgaagtcgt gcgcccggta
     1801 ctcgacggca tggcggcgga aggaacgccg ttcacgggga cgctctatgc gggtatcatg
     1861 tggaccgaag ccggaccgaa ggtcctcgag ttcaacgcgc gctttggcga ccccgaaaca
     1921 cagtcgttga tgccgctgct cgtcgagggg gacgtgtgcg gcctgctggc gtcggccgcg
     1981 aacggaaatc tgcccgaagg cgtgaaagcc gcggcgcgct cggccgcgac gatcgtggtc
     2041 gcgtcgaagg gctaccccga gagcgcgcag agcggcgtcg agattcgcgg ccacgacgcg
     2101 gccgaggagg gcacgatggt gttccacgcc ggcacgcggg tcgtggacgg gcgcgtggtc
     2161 accgcgggcg gtcgcgtgct cggcattacc gggtgggcgc ccacattgtc cgaggcgctg
     2221 gggcgcgcgt accgcgtcgc cgcgcgcatt cacatcgacg gcgcgtttta tcggagcgac
     2281 atcggacgaa ggcacttgta gaaaggaaac acgatggcga aggcaccgcg ggtagcgatt
     2341 ctcttgggca gcaagagtga cgaaggtcac ctcaaggcga cgaccgatct cctcgatcga
     2401 ttccagatcg actgggagct caaggtgatc tccgcgcatc gccagcccga acaattgcac
     2461 gagtacgtgc gcgccgcgga cgcgggcgga acgctcgtct tcatcgcggc ggccgggctc
     2521 tcggcggcgc tccccggcgt gatcggttcc atctcgcaaa agcccgtcgt cggattgccg
     2581 attccgggcg gtgccttggc cggcgtcgac gcgctcttgt cgatggtgca gatgccgggc
     2641 ggcattccgg tggcgacggt gggggtgggg tcgaacggct ccaagaacgc cgccatcctc
     2701 gcggcccaga tcatcgcgct cggcgacccc aaggtggccg agcggctacg cgcataccgc
     2761 gaagagctgc gcaccggatg acgccgcgag cctcgctcgc gggcttcatc gacgacccgc
     2821 gcgcgcaggt cctggccgcc gaatcgctcg tgcgcggcgc ggtcgtggtc ctgcccaccg
     2881 acaccctcta cggcctgtcc gccgcgctct cgcacgagga ggccgtgcgc cgcatcgcct
     2941 ccatcaagca agctccccag gaacgacggt tcatcgtcct cgcgtcgtcg atcgagatgg
     3001 tggagcgcta cgtcgcgtcc ttcgggtgcg cgacgcgcga ggatctcgcc gcgcgctggc
     3061 cggcgccgtt caccgccatc ctgccgtcgg ggcgcgcgtg tcccgcgtgg gtcggtccca
     3121 ccgtcgcggt gcgcgttccc gccttcgacc cgattcgcgc gctgatcgag cgcgtggggg
     3181 aacccatcgt gtccaccagc gtcaatcgca ccggccgcga gccgctggac gacgcgcccg
     3241 cgatcatgcg cgagttcggc gacgagatcg acgcggtgtt cgagcgaagg ggtgaaggcg
     3301 gcggcgcctc gaccatcgtg gacctgtgcg ggaagacggc gcgcgtggtg cgcgcggggg
     3361 gctacgcgtg ggacgcgacc gggggcggga agccttcgaa gtagtggccc ttgtaggcct
     3421 cgcagttcga gcacagcagg atttcgcggc gccggccctg ggccagcagg tcgcggtagc
     3481 gacgattcac ttcgccgttc caaatatcga ttacgttttc gcgcgtcgcg tcgccgatga
     3541 caccgcgctc gtgcgagtcc cagcagcaca gcgtggcctt gccgttgacg aagaagaaca
     3601 tctccttgag gaccttcttg cacgggagct gcaccggctt gacttccggc tcccacggcc
     3661 acacgtagag gtcggtgatc acggccacgg cgcccacctt ggtccagaac tcgacgaagg
     3721 gttcgtgctc gtggcggttg tagtccatcg cgatcatgcg cacctcgacg tgccccgcgc
     3781 taccgcgctc gcgcgccatc tccacgaagc ggcgcacgcg ctcgaccgtg aggtcgtagt
     3841 cgagcccgac gcgcgacttc gagaacgtct ccttgctgaa gccgtcgatc gagaagcgaa
     3901 tgatgtcgat gccgacgtcg agaatctcct gcgcgcgctc ttccttcatg agttcgccgt
     3961 tggagttgat ctcgatcttg gcggtgtcgt ctttgcgaat gtagcgcatg atctcgccca
     4021 gacggtggtc ggcgagcggc tcgttgatga gaaagggccg gtacgtgatc ccgagcccgc
     4081 gcgtctcgtc gatgatcttc ttccacagcc agtcttccat gcgccgcggg cgccgcgtga
     4141 gcgtggaatg cgagcaaaac gggcactcgg cgttgcacgc gagcgtggtc tcgatctgga
     4201 cgactttggg atgtgcaagt tggatcatga tctggggcgt cccgggaata ttacaggtag
     4261 ggggctagcg ccgccatgca tttctcggtg gcgcccagct gcgattcgac gtaggcgcgc
     4321 ccggccgcac ccgcgctctt gcgcgcggcc tcgtcgcgaa gccaggccgc gagagcgtgg
     4381 taggcctcgt cggcggtacg aatcacgcgc gcggcgccgc gctccaccag ctgcagcgct
     4441 tcgaacgagt tgtcgtgctt ggggccgaag accaccggca cgccggcgat ggcgggctcg
     4501 atcacactat ggacgccggt ggtgaaggcg cccccgacgt acaccagatc ggcaaagcga
     4561 tacgcttccg cgagcacgcc caccacgtcg acgatcacga tgcgcgcgga cgatcccgtc
     4621 ccggcggccc agcggcgcag cggctccacg tgcgcgggag tgggctcgtg cggaaccagc
     4681 acgacgcgca aacgcgcgtc ttcggagagc aagcgcgcga gcgcgggcag gagccgctcc
     4741 tcgtcgggcg gccacgtgct ccccgcgatt agtgtcggtc cgcccgcggg caacgcgaac
     4801 gtgctcgccg tgcgctgctg ccagcgatcc attacgcgat cgaagcgcgt gtcgccggtg
     4861 accgacaccg acggatgcga cggcaccgtc gcgcggaagc gcgccgcgtc gtcgtcgctg
     4921 atggcgagga tcgcgtccag tcgccgatag acgtcgcggt acaaccagcg cgcgggcggg
     4981 gccaggcgcc ccgacgacgc gctcaaggtg gcatcgatca ggacaatggg cacgccgcgc
     5041 gcgtccgcct cccagaccag attcggccac aggtcgaact tgacgaatac caataggcgc
     5101 ggcttcacct gctccaggca gaagcgcatg ttgggcgcgg tgtcgaacgg gagataatcg
     5161 atgaagcgaa gcgagccccg gcccggcgtc tcgcgcttgc gcgcgaaggc gtacccggac
     5221 ggcgacgaga acgtgagcac cacgggcagg tcggggcgcg cgcgctccag ggccgagatc
     5281 accggccgtg cctgctcgaa ctcgcccaca ctcgcgacgt gaaaccagac cggcggcgcg
     5341 ttcaagtcgc gcgcgagcgc ttcccagcgc cctcggaagc cgcgccgacc gtcgacggcg
     5401 gcgcgcatct tggggagcaa gcgcgcggcc gcgtgcgcgc cgagaacggc cgcgggaagg
     5461 agcgcgttgt acacccagcg cgcgcccgac atcagtcgac tacccggtga cgctcgcccg
     5521 gtccggggcg caatacggtg tgcaacacgt cgtcgacgcg aatgccgtcg aggcactcgt
     5581 aggtccgctt ggggcagggt gcggcaccgt tgcgggagca gggccggcag gcgagacggc
     5641 gctcgatggt agtgcttggc agaagacttg gggcgtaccc aaactcccgc acggtggggc
     5701 cgaagagtgc cgtgacggga acgccgactg cttcggcgag gtgcatcaag cccgagtcgt
     5761 tgcccacgaa cgattcgcag cgcgcgatca gcgccgcggt ttgaatcagc gtggtctgtc
     5821 cggcggcgtc gacgcagtgg ctcccgaggg acgcggcgat gggggccgtg aactcgcggt
     5881 cgcgcgcgtc gccgacgagg acgacgcggc gccccgccgc aacgagtccg tcgcacagcg
     5941 cggcgaaacc gccccagcgc ttcgcgcgcc agcggctgcc gggggcgacc gcgaccgcgg
     6001 gctggtcccc tcccacgcgc agcgacgccg tctcgctctc cgcgcgcggg atgcgcaggc
     6061 ctccgggcaa gagcgtcgtc gcaccgaagc cggcctgttc cgcgagcgcg gcatattgcg
     6121 cgaggatggg cggcagcgaa gcaggacgct tgaagacgat taggcccgcg cgctctcgat
     6181 agtatttccg gaagcgcgcg tgcgcgccgc ccgcggcggc ggacacgatg cggctgcgcg
     6241 ggttcgaatg cgcgtcgatg acgacgtcgc cgggtcgcgc gccctggcgg aaggagcgtc
     6301 cgatgcgcgc gaggcccaaa agcgacgtac ccggctcgat cgcgagcaca cggtcgacgc
     6361 cctcggcgtg cgcgaacacg tcggcgtagg cgcgcttggt cagcaccacc acttcggcgg
     6421 cgcccgcacg cttgaggtgg gcgaggagcg ggcacaagag cacgcagtcg cccagcgagc
     6481 tgaatcgaat gacgagaact ctcatctcga tgtgtcgggt gcgttgacgc ttgacaggtc
     6541 gcgccgcggg agagaaggtg cgaaggtgtc catgccgacc cactccttcg aactcggcga
     6601 gcgaatcaag aagctgcggc tttcgcagaa ccttacacta aagcaactcg aggcgaaagc
     6661 gggagtttcc gccacgcatc tttccgagat cgagcgcggg caatcgtcgc ccacggtggg
     6721 gacgctggag cgcgtcgcgc gcgccctggg cgaagagccc gcgctcctcg ccggcgagcg
     6781 aacgcctcgc cgggtggcgg tggtgcgccg caacgagcgt cgcgcgtggg ccgcgggcga
     6841 ggctcgcgtc gcctccctcg ggactccgct ggacggggcc gagatgtcgg tggtcgagat
     6901 cgagctcggg accggagaga cgggtttcga cccggggccc gtcgccaacg aagagttcct
     6961 gctggtcctc gcgggggcga tcgatctgcg ggtcgggtcg acgtcccacg cgctcgcggc
     7021 cggcgacgcc atccacctcg ccacgaacga gtcgcgacgg ctgcacgcgc gggaagcggc
     7081 gcgcgtgctc tggatcacac ttccggcttc gactctctag agaggacccg cacaatcgat
     7141 ggacgtcgga tcgagagtca agcgcgtgcg ccacgagaag gggctgaccc tcaaggcggt
     7201 cgagtccctc tcgggcgtgt cggccacgca cctgtcggag atcgagcgcg gcgagacctc
     7261 tcccaccctg ggctcgctcg cgcgcatcgc gcgcgcgctg tccaagccga ccgcctactt
     7321 cctggaggac aacgagctgg gcccagtgtc gcgcgtgggc gccaaggacc gcgtgcgcga
     7381 agcggtcaag ggctcggcgc ggcgggggac cgcgtcgatc gagcgcctga ccgccggcgt
     7441 tcccggcgga cgattgcacg cgcggcgcgt cgagctcccg cccgggagcg agtaccgcgc
     7501 cgagcgccac gcgcacgcgg gcttcgaggc gatgctcgtg ctcgacgggc gcgtgcgcgt
     7561 cgacgcgggc gaacaatcgt tcgacctcgc cgaaggcgac gcgattcatt tcgacgcgtc
     7621 gttgccgcac gcgtacgcca acgcgtcgcg cgacgaggcg gccgccctga tttgggtcgc
     7681 gacgcggcgc gacgtcgatt gaacgcgccc actgggcacg cttcctcgcg acgacgcggc
     7741 cgggagcctc aaaccgccca tgttttcttt tgacagcctt cgcgacccct tcctatgatc
     7801 gcgcgcgtac cagcgtacgg ccaatgcgat gaaaccagac gtctcacgcg acgagttact
     7861 gttccttcac ttggtgtcga tgctccagtt cgccgcgatg cagcagatgg gcaaagtgcc
     7921 gaacccggtc agcggagaga tcgagcgcga cctggagcac gcgcgcgcgt ccatcgacat
     7981 ggtggagatg ctgcacgcga agacatcggg acagcgaacg gcggccgagt ccgagttcat
     8041 cgacaaggtc ttgttcgaat tgcgcatgaa cttcgtcgac gagaccaaac gggtcgagaa
     8101 agagcgcgcc gggaccgagg gcatggcgcc ggacgcaccg ggtgcgggcg ggaccgacgc
     8161 cggccgctga cggccatctt ggaatcaatc ttgctctgcc ggttggattt tccggctaac
     8221 ggcaagaaag gagacggttc gactatgcaa ggattaccgc gttcgggccg catggggttg
     8281 ttcctcgtcc tggcggcgct ttttgcactg cccgaggggg cgaccgcgag cgcgtcgtat
     8341 aacggaacgc gcggcctgat tcgaaccaaa tcggccgaca cgtaccgcaa gggcagcctg
     8401 agcttccagc tttcctccaa ctatgggaag caggacaacg agcagctgtc ctcgggcttg
     8461 ttctttccgt tgggcgactc ggccaccgtc gattatcaca ccttcatcac gcgcgcgacc
     8521 atcacgtatt cgttgagcga tttctttgag atcgcgggcg acctcgaggt gcgcaactgg
     8581 attcgcacca tccaggagca gagcccgggt gcgagcgaag acacctacac gcgcggcggt
     8641 ttgggtgaca ccgacgtctc cgccaagctc aacattccgt tgccgtcgaa gaagttcaaa
     8701 ctgggcgcct acggcaacat ggtgtttccg accggcgaca agaacaaggg cttctccatc
     8761 gacgccaacg acatcaccat cctggggttg gccaccatcg acatgtccga cctccagagc
     8821 ttcgtgccca cgcgcctgca catcaacgcg ggctaccgtc tcaacaaggg cgaagtcccc
     8881 acggactcgc tgggctacgg catcttcgac cccaacttcc cggatatttc gggcttcaac
     8941 ccgccgggtt acccccctgt cccgaccggc gagagcgact cctacaacga cgccttcctg
     9001 ttcaacgcgg cgctcgagtt tccggcgccg tcggtgacgt tcttcgtcga gttcgacatg
     9061 aatcagctcc tgaacgtgga cctgcccgcg aacgcgggtg tgaacgcgag cacctacacg
     9121 ctcaccccgg gcgtggagtt caagggcaag gggtcgtcgc tcgaattggg cgccgacatc
     9181 aacttgaact cgggcgacaa cccgtcgctg gtcaatccgc ccgactgggg gctgtggatc
     9241 gcgttcaact acgtggccga ggtcgtgccg cgcgactccg acaaggacgg tatcgccgac
     9301 aacgtcgacg gttgcccgga cgaagccgag gacatcgacg gcaaggacga caaggacggc
     9361 tgcccggacc tcgacaacga cggcgac
//
LOCUS       DASPGK010000419         5491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1388636, whole genome shotgun sequence.
ACCESSION   DASPGK010000419 DASPGK010000000
VERSION     DASPGK010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388636"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VEC56_11590"
     CDS             complement(<1..872)
                     /locus_tag="VEC56_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HXV14838.1"
                     /translation="MPVTLVVGGQWGDEGKAKIIDYLARDVDFVVRYQGGANAGHTVV
                     VGTEKFAFHQVPSGILYPKVTCVLGGGMVIDPLALVAEIDGIVARGVDVRGRILVSSQ
                     AHLVLPYHVMLDCDSEDRRAEGAIGTTRKGIAPAYADKARREGIRMADLMRKPASLRD
                     LVTDRVRENNKILKARGMKPLSPSKVAEGVLAVRARLAPLVADTRPVLWDAVAAGRRV
                     LCEGAQGSLLDIDHGTYPYVTSSSCTAGGAAIGTGLPPSAFDRIVGIYKAYCTRVGNG
                     PFPTEEKGGVGQRLR"
     gene            complement(1022..1972)
                     /locus_tag="VEC56_11595"
     CDS             complement(1022..1972)
                     /locus_tag="VEC56_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXV14839.1"
                     /translation="MSRVLLADGIAADAVRVLQSFGGIEAVAMGTPSPAELIELIPGF
                     DAIVVRSPTRVTAEVIERGTKLRFIGRAGVGVDNIDLEAATRRGIVVMNSPRSNTIST
                     AEHTLALMLAVAREVPRAHATVSAGKWDRDSFRGVELYGKTLGVVGLGRVGREVATRA
                     AAFGMTVLGHDPLVGVDAARAVGARWVALPELLAQSDWVTLHVPLSDATRRMIDARAI
                     AAMKKGVVVVNCARGGIVDETALADALDAGHVAAVAFDVFEKEPPGTSRLFAHPRSVF
                     APHLGAATVDAQRRVATDVAESIGLALTRGEIRDAVNAPR"
     gene            complement(1969..3111)
                     /locus_tag="VEC56_11600"
     CDS             complement(1969..3111)
                     /locus_tag="VEC56_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXV14840.1"
                     /translation="MRSPKLFTPGPTAVPADVLETQARPLIHHRTDEFRHALTESMRG
                     LQYVLETKNPVVILTASGSGAMEATVLNLTQPGETVIVTEVGKFSERWREIAEVYGVR
                     VVAVRAEYGQVASANDVERAFKQHPGASVLFTTHSETSTGALQDVAAHAKVARAHGAL
                     VAVDAITSAGAHHVRTDAWDLDAVVGGSQKGVMIPPGLGYVALSPRAIAKMRAGRHAV
                     YYFDLLKAVASAEKGDTPYTPAITIVLALKRALEMMREEGIENVIARHDANASAVRAA
                     VKAMGMELLAVIPSNAVTAVVTPGDSAGQITKHIETRYGVKIAGGQGSLKGKIVRLGH
                     LGYYDESDMYTLASAFEATLNDLGLVKTFGAGVAALRSSYQARSAR"
     gene            complement(3205..3792)
                     /gene="yihA"
                     /locus_tag="VEC56_11605"
     CDS             complement(3205..3792)
                     /gene="yihA"
                     /locus_tag="VEC56_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXV14841.1"
                     /translation="MRIQTVEFVGSFGYPGPLPDPRRPEVVFFGRSNVGKSSLINTIL
                     GRGGVARVSKTPGKTRAANFFVVNKTFMLVDMPGYGYAKVSKGEIERLRRLREQYLDA
                     EDRDAALVQLIDARHLPTALDVESVARLSESGRPLCLVFTKADKVAKSAQKGTLARAV
                     ARLEVRPDTGVVAFSSVSGQGKLELWAWIEAHLGA"
     gene            3878..>5491
                     /locus_tag="VEC56_11610"
     CDS             3878..>5491
                     /locus_tag="VEC56_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV14842.1"
                     /translation="MTHVTPESIAATGVAAPQELLHRFHTLARGLDATDAWRRVSREL
                     LRPEHPHALHRLLFEAVFASWPAANGPPPAWTPTPAEVASTHIAGACRRLRLPDYAAF
                     HRWSVENRAEFWGDFIETRGIAFRKAPERTVDLARGPEYPRWLPGARMNVAQSCFLAP
                     PDTTAIVFQKDAGAIESISYAELDALTNRFANALAEFGAKRGDAVAIAMPMTVEAVVA
                     YVGLVKAGCVVVSIADSFAPEEIRTRMEIARASLAVTQDVVVRGDRTLPMFDKLVAAG
                     ARACVVVARDGAPSVTLRAADRRWGDFLSARDRFDPVAAAPEDTTNVLFSSGTTGEPK
                     AIPWSHTTPLKCAMDGYYHHDIREGDVVAWPTNLGWMMGPWLIYASLINRATIGLCYH
                     APNTSAFCRFVADARVAMLGVVPSLVKAWRAHGMTDGIDWTPLRAFSSTGEASSADDY
                     LWLMARAGYKPVVEYCGGTEIGGGFITQSLTQPAAPALFSTPSLGLDLAILDEEGRST
                     SHGELFVVPPSIGLSLSLLNRDHHEVYYAG"
BASE COUNT          830 a         1959 c         1829 g          873 t
ORIGIN      
        1 cggaggcgct ggcccacgcc gcccttctct tcggtcggaa acggtccgtt gccgacgcgc
       61 gtgcagtagg ccttgtagat cccgacgatg cggtcgaagg cggaaggcgg cagcccggtc
      121 ccgatcgccg ccccgcccgc cgtgcacgac gacgaggtga cgtacggata ggttccgtgg
      181 tcgatgtcga gcaacgagcc ctgggcgcct tcgcacaaca cacggcgtcc cgcggcgacc
      241 gcgtcccaca acacgggccg cgtgtcggcg accagcggtg ccaagcgcgc gcgcaccgcg
      301 aggactccct cggcgacctt ggaaggcgag agcggcttca tgccgcgcgc cttgaggatt
      361 ttgttgttct cgcgcacgcg gtcggtgacc aaatcgcgca agctggccgg cttgcgcatg
      421 aggtcggcca tgcgaatgcc ctcgcggcgc gccttgtcgg cgtaggctgg tgcgataccc
      481 ttgcgcgtcg tgccgatcgc gccttctgcg cgccggtctt cggaatcgca atcgagcatg
      541 acgtggtacg gcagcacgag gtgggcctgc gagctgacca ggatgcgtcc gcgtacgtcg
      601 acgccgcgcg ccacgatgcc gtcgatctcg gccacgagcg cgagcgggtc gatcaccatc
      661 ccgcccccca agacacacgt caccttgggg tagagaatcc ccgacggcac ctggtggaac
      721 gcgaacttct cggtccccac cacgaccgta tgaccggcgt tggcaccacc ctggtagcgc
      781 acgacgaagt cgacgtcgcg ggccagatag tcgatgatct tggccttgcc ctcgtcgccc
      841 cattgtccgc cgacgaccaa cgtgaccggc attcgcaacc actcctcgcg cgcacgcggc
      901 gcgcacaaaa atccgccccc actggaggcg gtgccattcg aacctgccgc ggcgcccgcg
      961 gcgttaccgg accgtcatta taggtggcgg gccgggaacg gtcaaccggc cgggcgcgcc
     1021 gctaacgcgg cgcgttcacg gcgtcgcgga tctcgccccg ggtgagcgcc agcccgatcg
     1081 attcggcgac gtcggtcgcg acccggcgct gcgcgtcgac ggtggcagcc cccaggtggg
     1141 gcgcgaagac cgagcgcggg tgcgcaaaca gacggctcgt gcccggcggc tccttctcga
     1201 acacatcgaa cgcaaccgcc gcgacgtgcc cggcatccag cgcgtccgcg agtgccgtct
     1261 cgtcgacgat gccgccgcgc gcgcagttga ccacgaccac gcccttcttc atggcggcga
     1321 tcgcgcgggc gtcgatcatg cgacgcgtcg cgtccgagag cggcacgtgg agcgtcaccc
     1381 agtcgctctg tgcgagcagc tcgggcagtg cgacccagcg cgcgcccacc gcgcgcgccg
     1441 cgtctactcc caccagcggg tcgtgaccca gtaccgtcat cccgaaggcc gccgcgcgcg
     1501 tggccacctc gcggcccacg cgccccaggc cgaccacgcc cagcgtcttt ccgtacaatt
     1561 cgacgccgcg aaacgaatcg cgatcccact tgccggcgct gacggtggcg tgcgcgcgcg
     1621 ggacttcgcg cgccaccgcg agcatcagcg cgagcgtgtg ctcggcggtc gagatggtgt
     1681 tgctgcgcgg cgagttcatc accacgatgc cgcggcgcgt ggcggcctcg aggtcgatgt
     1741 tgtcgacccc gactcccgcg cgtccgatga agcggagctt cgtcccgcgc tcgatcacct
     1801 ccgcggtgac gcgcgtcggg cttcgcacca cgattgcgtc gaagccggga atcaactcga
     1861 tgagctccgc gggagacggc gtccccatcg cgaccgcctc gatgcccccg aaggattgca
     1921 gcacgcgaac cgcgtcggcg gcgatgccgt cggcgaggag gacgcggctc accgcgcgct
     1981 ccgcgcctgg tacgagctgc gcagcgccgc gacacccgcg ccgaaggtct tcaccagacc
     2041 gaggtcgttc agcgtggctt cgaacgcgga ggccagcgtg tacatgtcgc tctcgtcgta
     2101 gtagcccagg tgcccgaggc ggacgatctt gcccttgagc gatccctggc cacccgcgat
     2161 cttgacgccg tagcgcgtct cgatgtgctt ggtgatctgg cccgcggaat cgcccggtgt
     2221 caccaccgcc gtgaccgcat tggaaggaat gaccgccaac agttccatgc ccatcgcctt
     2281 gaccgccgcg cgcaccgcac tcgcgttggc gtcgtggcgc gcgatcacgt tctcgatccc
     2341 ctcttcgcgc atcatctcca gcgcacgctt gagcgccagc acgatcgtga tcgcgggcgt
     2401 gtagggcgtg tcgcccttct ccgccgaagc cacggctttc agcagatcga agtagtagac
     2461 ggcgtggcga ccggcgcgca tcttcgcgat cgcacgcggc gagagcgcca cgtagcccag
     2521 gccgggcgga atcatcacgc ccttttgcga gccccccacc accgcgtcca agtcccacgc
     2581 gtcggtgcgc acgtggtgcg cgcccgcgct ggtaatggcg tcgacggcga ccagggcgcc
     2641 gtgcgcgcgc gcgaccttcg cgtgggcggc gacgtcctgc agagcgcccg tcgaggtctc
     2701 cgagtgggtc gtgaacagca cactcgcgcc cgggtgctgc ttgaacgcac gctccacgtc
     2761 gttcgccgac gcgacctgtc cgtactccgc gcgcaccgcg acgacgcgca cgccgtagac
     2821 ttcggcgatc tcgcgccagc gctcgctgaa cttccccacc tcggtgacga tcaccgtctc
     2881 gccgggctgg gtcaggttga gaacggtcgc ttccatcgca cccgaccccg atgcggtcag
     2941 gatgaccacg gggttcttcg tctcgaggac gtattggagc ccgcgcatcg actcggtgag
     3001 cgcgtggcga aactcgtcgg tgcgatggtg gatgagcggc cgcgcctgcg tctccagcac
     3061 gtcggcgggg acggcggtcg ggccgggggt gaacaacttc ggcgatctca tggtgattcg
     3121 atggctccgg tgataagagg tgaaaacaac cgccggatta tacggcgcgc gcggggccgc
     3181 ttgaaagggc gaacccggcc gcgttcaggc cccgaggtgg gcttcgatcc acgcccacaa
     3241 ctccagcttg ccctggccgg aaacgctcga gaacgccacc acgccggtgt cggggcgaac
     3301 ctcgaggcgc gcgaccgccc gcgccagcgt tcccttctgc gccgacttgg ccaccttgtc
     3361 cgccttggtg aacacgaggc acagcgggcg ccccgattcg gagaggcgcg ccaccgattc
     3421 cacgtcgagc gcggttggaa ggtggcgtgc gtcgatcaat tggaccagtg ccgcgtcgcg
     3481 atcctcggcg tccagatact gctcgcgcaa tcggcgcagc cgctcgatct ctcccttgga
     3541 caccttcgcg tagccgtacc ccggcatgtc gaccagcatg aaggtcttgt tgacgacgaa
     3601 gaagttcgcg gcgcgcgtct tcccgggcgt cttggacacc cgcgcgactc cgccgcgacc
     3661 gaggatagtg ttgatgagcg acgacttgcc cacgttggag cggccgaaga acaccacttc
     3721 cggccgccgc gggtcgggga gcgggcccgg gtaaccgaac gaccctacga attcgacggt
     3781 ctggattctc atagaaacgc gtttgcggtt ggaggctgca tcgccgcgcg attataatga
     3841 ccgtccggcg tttcgtcgct atcgttgttc gacccgcatg acccacgtca ccccagaatc
     3901 catcgctgcg accggtgtgg ccgcgccgca agaacttctg caccgattcc acacgctggc
     3961 gcgcggcctg gacgcgacgg acgcgtggcg gcgcgtttcg cgcgagctgc ttcgccccga
     4021 acacccgcac gcgctgcacc ggcttctctt cgaggcggtt tttgcgagtt ggccggctgc
     4081 caacggcccc ccgcccgcgt ggacgcccac acccgcggag gtcgcgtcca cccacatcgc
     4141 cggcgcctgc cgccgcctgc gccttcccga ctacgccgcg ttccaccgct ggtcggtcga
     4201 gaaccgcgcg gagttctggg gcgacttcat cgaaacgcgg gggatcgcct ttcgcaaggc
     4261 gccggagcgc acggtcgatc tcgcgcgcgg ccccgagtac ccgcgctggc tcccgggcgc
     4321 gcgcatgaac gtcgcccaga gctgctttct cgccccgccc gacaccaccg ccatcgtgtt
     4381 ccagaaggac gcgggtgcca tcgaatcgat ttcctacgcc gagctggatg cgctcaccaa
     4441 ccgcttcgcc aacgcgctgg ccgaattcgg cgcgaagcgc ggcgacgcgg tcgcgatcgc
     4501 gatgccgatg acggtcgaag cggtggtcgc ctacgtgggc ctggtcaagg ccgggtgcgt
     4561 ggtggtgtcg atcgccgaca gcttcgcgcc cgaagagatt cgcacgcgca tggagatcgc
     4621 gcgcgcgtcc ctcgccgtca cccaggacgt ggtcgtgcga ggcgacagga cgctgcccat
     4681 gttcgacaaa ttggtcgcgg cgggcgcgcg ggcgtgcgtg gtggtggccc gcgacggcgc
     4741 gccgagtgtc acgcttcgcg ccgccgaccg tcggtggggc gactttcttt ccgcccgcga
     4801 tcgcttcgac ccggtcgcgg ccgcccccga agacaccacc aacgtgttgt tctcgtcggg
     4861 cacgaccggc gagcccaagg cgattccctg gtcgcacacc acaccactca agtgcgcgat
     4921 ggacggctat taccaccacg acatccgcga gggcgacgtg gtggcgtggc cgaccaacct
     4981 ggggtggatg atggggccgt ggctcatcta cgcgagtttg atcaatcgcg cgaccatcgg
     5041 cctgtgctat cacgcgccca acacgagtgc cttctgccgc ttcgtggccg atgcgcgcgt
     5101 cgcgatgctg ggcgtcgtgc cgagcctggt caaggcgtgg cgggcgcacg ggatgaccga
     5161 cgggatcgat tggaccccac tgcgcgcgtt cagctcgacc ggtgaagcgt cgagcgccga
     5221 cgactatctg tggctcatgg cgcgcgcggg ctacaagccc gtggtcgagt attgcggcgg
     5281 gacggaaatc ggaggcggat tcatcacgca gtcgctgacc cagcccgcgg cgccggcgct
     5341 gttttcgacg ccgtcgctgg ggctcgacct cgcgatcctc gatgaggagg gccggtccac
     5401 ttcacacggc gagttgttcg tggtaccgcc gtcgatcggg ctctcgctct cgctcttgaa
     5461 ccgcgaccac cacgaggtct actacgccgg c
//
LOCUS       DASPGK010000420         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1506859, whole genome shotgun sequence.
ACCESSION   DASPGK010000420 DASPGK010000000
VERSION     DASPGK010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506859"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VEC56_11615"
     CDS             complement(<1..245)
                     /locus_tag="VEC56_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV14843.1"
                     /translation="MSHQFVCTLYKLKKVVPPDRVILDDITLAFFPGAKIGVLGHNGA
                     GKSTLLRIMAGVDTEIQGECQTLPGLKIGYLPQEPQL"
     gene            complement(371..805)
                     /locus_tag="VEC56_11620"
     CDS             complement(371..805)
                     /locus_tag="VEC56_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HXV14844.1"
                     /translation="MTMAAGARWEHFPHGADIGIRGLGPTLDAAFEQVALALTAVMTE
                     PARVEARETIEVSCTASNPDDLLYDWIDAIVFEMATRGMLFGAYDVRIEGNRLASRLR
                     GERVDRARHEPAVEVKGPTYTQLHVSHDEARDEWVAQCVVDV"
     gene            complement(802..>2124)
                     /locus_tag="VEC56_11625"
     CDS             complement(802..>2124)
                     /locus_tag="VEC56_11625"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXV14845.1"
                     /translation="EMDDKMLEQATNVATLPGIVGASYVMPDGHWGYGFPIGGVAAFD
                     PDAGGVVSAGGVGFDISCGVRCVATGLKVDRIRDVQEKLADALFEHVPAGVGSHGAIH
                     LDDAGMDAMLRGGARWALERGWGVHGDLDRIEEKGRASGALPEAVSDRAKERQRGEMG
                     TLGSGNHYLEVQRVSHVFDADAAAAFGLAVDDAVISIHCGSRGMGHQIGTDYLREMER
                     SAPQHGLKLLDRELACAPLSSELGKRYLGAMRAGINCALANRQIILHLARGVFADVLP
                     EATLTLVYDVSHNTCKEEEHVVAGRKRRLFVHRKGATRAFGPGHSELPNEFRDAGQPV
                     LIGGSMGTESWVLAGTREAMQLSFGSACHGAGRRMSRHQATRQWQGRGVIRELAQRGI
                     SIRAVGIRGVAEEAPGAYKDVSVVVDAAHRAGLARKVARLEPVICVKG"
BASE COUNT          347 a          743 c          697 g          337 t
ORIGIN      
        1 tcgagctgcg gctcctgggg cagatagccg attttcagcc cgggcaacgt ctggcactcg
       61 ccttggattt ccgtatcgac gccggccatg atgcgcaaga gcgtgctctt gcccgcaccg
      121 ttgtggccga ggacaccgat cttggcgccg gggaaaaacg ctagcgtgat gtcgtccagg
      181 atcacgcggt cgggcgggac gaccttcttg agcttataga gcgtacaaac gaactgatgc
      241 gacacagggg tccttcttgt ggttcacatg tgaatgggtg gcaaaaaaga gaagggatga
      301 taccccaaag tccgatggaa tggtagcacg cgcagccggg tcaagcgtgg caggagcgaa
      361 gaaatggccg ctaaacgtcg acgacgcact gcgcgaccca ctcgtcgcgc gcctcgtcgt
      421 gtgacacgtg cagctgcgtg tacgtcgggc ccttgacctc gaccgccggc tcgtggcggg
      481 cgcgatcgac gcgctcgccg cgcaaccggc tcgcgagacg gttcccttcg atgcgcacgt
      541 cgtacgcgcc gaacagcatc ccgcgcgtcg ccatctcgaa aacgatggcg tcgatccagt
      601 cgtagaggag gtcgtcgggg ttggatgcgg tgcacgagac ctcgatggtc tcgcgcgctt
      661 ccacccgcgc gggctccgtc atgaccgcgg tgagcgcgag tgccacttgc tcgaaggcgg
      721 cgtcgagagt cggccccagg ccgcgaatgc cgatgtcggc gccgtgcgga aagtgctccc
      781 agcgcgcgcc ggccgccatc gtcatccctt gacgcagatg accggctcca ggcgggccac
      841 cttgcgcgcg agcccggccc gatgcgcggc gtcgaccacc acggagacgt ccttgtacgc
      901 accgggggct tcttcggcca caccgcgaat gcccaccgcg cggatcgaga tcccacgctg
      961 cgcgagctcg cgaatgacac cgcgtccctg ccactggcgc gtggcctggt gccggctcat
     1021 gcggcgccca gcgccgtggc acgcggaacc gaacgagagc tgcatggcct cgcgcgtccc
     1081 ggccagcacc cacgattccg tccccatcga tccaccgatc aagaccggct gaccggcgtc
     1141 gcgaaattca ttgggaagct ccgagtggcc ggggccgaac gcgcgcgtgg ctcccttgcg
     1201 atgaacgaac aaacgtcgct tacgtcccgc gaccacgtgc tcctcttcct tgcaggtgtt
     1261 gtgcgagacg tcgtagacga gtgtcagcgt cgcctcggga agcacgtcgg cgaagacgcc
     1321 gcgcgcgagg tgcaggatga tttgccggtt ggcgagcgcg cagttgatac cggcgcgcat
     1381 cgcaccgagg tatcgcttgc ccagctccga ggagagcggc gcgcacgcga gctcgcggtc
     1441 caggagcttc aatccatgct gcggcgccga ccgctccatc tcacgcaggt agtcggtgcc
     1501 aatttgatgg cccatgccgc gcgacccgca gtggatgctg atgaccgcgt cgtcgaccgc
     1561 gagcccgaag gcggccgccg catcggcgtc gaagacgtgc gaaacgcgct ggacttcgag
     1621 gtagtggttg cccgagccca gcgtccccat ctcgccgcgc tggcgttcct tggcccgatc
     1681 cgagaccgct tcgggcaggg ccccgctcgc gcggcccttc tcttcgatgc gatcgaggtc
     1741 gccgtggact ccccacccgc gctcgagtgc ccagcgcgcg ccgccgcgca gcatcgcgtc
     1801 catgcccgcg tcgtcgaggt ggatggcgcc gtggctgccc acgccggcgg gaacatgctc
     1861 gaagagcgcg tcggccagct tttcctgcac gtcgcgaatg cggtcgacct tcaacccggt
     1921 ggccacacag cgcacgccgc acgagatgtc gaagcccacg ccgcccgccg acaccacccc
     1981 gcccgcgtcg gggtcgaacg ccgccacccc gccgatcggg aatccgtagc cccagtggcc
     2041 gtccggcatc acgtacgacg cccccacgat gccgggcaga gtcgccacgt tggtcgcttg
     2101 ctcgagcatc ttgtcgtcca tctc
//
LOCUS       DASPGK010000421        11021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_922244, whole genome shotgun sequence.
ACCESSION   DASPGK010000421 DASPGK010000000
VERSION     DASPGK010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922244"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /locus_tag="VEC56_11630"
     CDS             complement(<1..2250)
                     /locus_tag="VEC56_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14846.1"
                     /translation="MNEKAASAQQKAMQERKRGHFDKAVKRLEQTIAQFPDELDLYLD
                     AVDACLEGGEVMQATNFLKTAQEKFTKDKDRVGQFVRDKLLAVHDPALARYVVENAVK
                     RRDLDAALEHLEQIPDHVARDLLARTKTKKQSLKSASHGGYSLRAEIFTNDLLSALLS
                     VRLGSMKEGMAALVEIIDEKPVETKMLLPFLAGLESKHAKVGRVRFAHACALWSNGNE
                     NDAIARIVEAARYDSTAIPLCVDRLTSLRDKTKFPPKVERALAEVLLLKGDFDAAAPV
                     LRGYMADNKDTGREVIMLLKPYIDPAHGVNECVWVALDAALGLDQSAVAMEILRPLHQ
                     RGNCTPEVYSRLQERSKDGLFTTEVMLFHASLALELKDFPRAVELLNIVVTSSPQDVH
                     SVVGLADKHRTSHPGLEELCRKHMAPEVVEAIAEEGDFQNFDHNEFSLRRDAKADGLE
                     RSSSAPRPSSPKPQGEKPASRFAEERKKPAEKQSFIETRELSFDDEGTIEGQVPDLPV
                     ENPSDEVDADVFETSREVSFSTPRKEKPAQEPMFKAPEQTPDLAPDPEPEREPEPVVE
                     HVVEQPAVTAFSAPQARPAVKMRASTEAGVTESHVNNVARRLGEVGAAAFFHIDDEPE
                     VPATPPEPAPAARAPIDAMSYVSPVVRPSAPEPVVSLPVEEPVEAEAPAASFEPIEAP
                     EPVVAVEPVETPAPAVAIEPFDSPAPAPKTFDSELARFRKGELSSAESVALVEQAVEI
                     GRVEDLHDLL"
     gene            2495..2938
                     /locus_tag="VEC56_11635"
     CDS             2495..2938
                     /locus_tag="VEC56_11635"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXV14847.1"
                     /translation="MPTVAYPRVVVYDSAAVRSPDMTGTFTLSVQCRLASSHVLAGCP
                     PCDRLHGHTWTVRAYWEFAALDEHGMGANFRDLKAVLRREVHDRFDHRHLNDIAPFDR
                     VPPTAENLAREVFTILRERFNPGPRGALARVEVWEGPEACAAYAE"
     gene            2949..3614
                     /locus_tag="VEC56_11640"
     CDS             2949..3614
                     /locus_tag="VEC56_11640"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HXV14848.1"
                     /translation="MTKPRSIALVSGGLDSLVSLARAVVERDVRIVMFFDYGQRARAS
                     ERVSAMSAANYYGLPFLDADVRWLESLSPSGMRSSDPDSEAGLATLDDVWIPNRNGVF
                     INVAAAYAESYGCDTVVTGFNREEAEEFPDNSREYVERVNAALALSTRNRVRVESFTI
                     DLDKRAIIRMGLESKAPLSIVWSCYRSGPLMCGRCASCARLRGAIDSLPPAERPVIEF
                     ADV"
     gene            3611..4165
                     /locus_tag="VEC56_11645"
     CDS             3611..4165
                     /locus_tag="VEC56_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HXV14849.1"
                     /translation="MTFAYRIIEGETAYTGVELRSGWVAERTGLSGDAAAGFVGKCRV
                     PNENLVDLDDARAGFFIAAASMAHVIAEHACTLEVAVLRQRLLVALLGEILRKRGKRM
                     RREGDDLYYDDRKLTVSIAAPSSATCLIHLGINIDPAGAPVPAVGLTEMRLDPREILN
                     ELLANYQDELASVAHATTKVRTVP"
     gene            4165..5019
                     /locus_tag="VEC56_11650"
     CDS             4165..5019
                     /locus_tag="VEC56_11650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HXV14850.1"
                     /translation="MSTNSEQNHTADAREIAPASTAAAGYLSEMFCSVQGEGLFVGER
                     QIFVRTAGCAATCSWCDTVYSKVQTPRFVVHGEGETPARWRANPVALEEVLDDVVACA
                     RANAPVVTVSVTGGEPLEQPEFVAALSRGLRERGFRVYLETAGIHPDALHTVVDHVDV
                     IAMDIKLPSATGVAHWDAHREFFSVLRGSEFDPARGARACFVKVIVDLKATVAEIERA
                     AELVASFSPRVPFVLQPESETMLSKNATRENRRALLELIDLAARAASLRLDSVRVIPQ
                     THKVLNVR"
     gene            5016..5699
                     /locus_tag="VEC56_11655"
     CDS             5016..5699
                     /locus_tag="VEC56_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXV14851.1"
                     /translation="MNADFLTAAVGLVAFIFSVIVHENAHGLAAERFGDPTARDLGRI
                     TMNPIPHIDPIGSILLPLAAFLSGIPFLGWAKPVPVNGANLRNPVVHSAYVAAAGPAS
                     NFLLAFAAAFLWIAVGLVFKHVPGLMETGERSLYFFNTLCSSLILINCVLAMFNLLPI
                     PPLDGHWILMRYLPPGPREALASVGRWGFFILILLLWSGVLWRILGPPLSLVVSGYHA
                     LVTTVVRAL"
     gene            complement(5765..6205)
                     /locus_tag="VEC56_11660"
     CDS             complement(5765..6205)
                     /locus_tag="VEC56_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXV14852.1"
                     /translation="MAIRKHLVLACALLAVSLVAFGCAEDAAAPQPSEDEAPVLAPTN
                     LQAVVINGGDIQISWNASSQPNVRGYNVYRVDVLESSIERLNAAPLEVTSVVDGGARF
                     GREYDYRVTSVSTRDTESRFAVVTIRNREAPPSDRRGDNPGEID"
     gene            6703..6981
                     /locus_tag="VEC56_11665"
     CDS             6703..6981
                     /locus_tag="VEC56_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV14853.1"
                     /translation="MTKAEIVEDIASKTGLTKKDVATAMDMFLESISKALSEGKHYEI
                     RGFGTFKVKKRKSRMARNPRTGESVPVPDRKVPVFKVSKELKDLVANV"
     gene            7059..8102
                     /locus_tag="VEC56_11670"
     CDS             7059..8102
                     /locus_tag="VEC56_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HXV14854.1"
                     /translation="MPEARRFIDLRSDTVTRPTAAMRRAIAEAEVGDDVFGDDPTVIA
                     LEKRIATLLKKESALFVPSGTMGNFLGVASLTETGDEVILERQSHIMNYEVSSVAALH
                     GVQLNALDGDRGVLTAAQIEPYIKTQSLHTPGTKVIAMENTHNRAGGRVYPFDEMQRV
                     KKLAETHGIRVHLDGARLCNAHVETGVSLADYAACADTVSMCFSKGLGAPVGSILVST
                     QDVVERARRLRKRLGGGMRQAGLLAAAALHALDHHVERMHEDHKNAKRLASFVEQVRG
                     LSLLFPVETNIVVARVDEKVFTVDALLAKLREHGVLAVPFGAGRVRMVTHLDVSSDDI
                     ERAGEALLALGRG"
     gene            8115..8843
                     /gene="rsmI"
                     /locus_tag="VEC56_11675"
     CDS             8115..8843
                     /gene="rsmI"
                     /locus_tag="VEC56_11675"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXV14855.1"
                     /translation="MGDSPGTLTLVATPIGNLGDITYRAIEVLKGADVVLAEDTRHTR
                     ILLNHYGIATQLLSYHDHNEARVTPSIIERLRRGENVALVTDAGTPAISDPGFYLVRA
                     ALEAGIHITMAPGANAVLPALVLSGFPCEAFVFAGFAPRKPGELKRAVETLESEPRTT
                     VFYVSPHQLHKVLDALAARLPERSIAVAREMTKVHEEVARGTAVDVRDHFAKSAPRGE
                     FVVVVKGIGKRRRVADDDAESAQP"
     gene            8878..9486
                     /locus_tag="VEC56_11680"
     CDS             8878..9486
                     /locus_tag="VEC56_11680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXV14856.1"
                     /translation="MDKRALQASARKILEPVVNVLVAFHISPLLVSIFGLAFSVWGGI
                     VVARGWLLAGGIFLLLAGLCDVLDGDVARRLGKASRFGAFIDSMLDRVSEFVYFGGVL
                     MYVVRRPSGFEVYEPVVIMLALMGSVLTSYARARAEGVGIECKVGVMERPERIALLAL
                     GLILGYRFLMAVMILLAVTSLYTVFQRIRHVHRAASDSTHRT"
     gene            9680..10465
                     /locus_tag="VEC56_11685"
     CDS             9680..10465
                     /locus_tag="VEC56_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14857.1"
                     /translation="MELSRLRAWAQGLATVLLLSAAVAGAQPALPHENHYNVYRTIPL
                     TYTRPITLIDQFGPIDVSLFVLDKFANPAEKRIPETGDVYPIINPDIHQLWWRIDAPQ
                     PARTIVGIDQFGIAQWHLGDAAYLLNPALKNVPPPAAPPEWNHYLCYHAQGPTVGRPV
                     IVIDQFGTSNVVVLYGKLFCNPVEKRHADGITYPIVDPVAHLACYLVQNATPFTRSIT
                     AIDQFGYWQLETFENDCLCLPALKEHVLKTQESTWGKIKAMYQ"
     gene            complement(10539..>11021)
                     /gene="tmk"
                     /locus_tag="VEC56_11690"
     CDS             complement(10539..>11021)
                     /gene="tmk"
                     /locus_tag="VEC56_11690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXV14858.1"
                     /translation="IRRLLTGQERASDGSVYRPGEDVMGLLFAADRLAHSRAIASHLA
                     AGEHVICDRYVFSSMAYQTLDSSISAEWVIDVNQGCAVPDLTIFLSVPVEVCLQRLSS
                     RKGTAAIYETRALLETIARNYETLLPRYETHFGRVVTLDGTRSVDDVHAALVEALDWE
                     "
BASE COUNT         1771 a         3656 c         3650 g         1944 t
ORIGIN      
        1 gagcagatcg tgcagatctt cgacgcggcc gatctcgacc gcctgctcga cgagcgcgac
       61 cgactcggcg ctcgacaact ctcccttgcg gaatcgcgcg agctcggagt cgaaggtctt
      121 gggcgcgggc gcgggtgaat cgaagggctc gatcgccacg gcgggcgccg gcgtctcgac
      181 cggttcgacc gccaccaccg gctccggggc ttcgatgggc tcgaacgacg ccgcgggggc
      241 ttcggcttcg accggctctt cgaccggaag cgaaacgacc ggctccggcg cggacggccg
      301 cacgaccggg ctcacgtacg acatcgcgtc gataggcgcg cgcgcggcgg gcgccggctc
      361 gggaggagtc gcgggcacct cgggctcgtc gtcgatgtga aagaacgcgg ccgctcccac
      421 ttcgcccagg cggcgcgcga cgttgttcac gtgcgactcg gtcacgccgg cctcggtgct
      481 cgcgcgcatc ttgaccgcgg ggcgggcctg cggcgccgag aaggcggtga ccgcgggctg
      541 ctctaccaca tgctcgacca ccggctcggg ttcccgctcc ggctctgggt caggagcgag
      601 gtccggcgtt tgctcgggcg ccttgaacat cggctcttgg gccggctttt ccttgcgcgg
      661 ggtcgagaag ctcacctcgc gcgaagtctc gaacacgtcg gcgtcgactt cgtcgctcgg
      721 gttttcgacc ggcaggtcgg ggacctggcc ctcgatcgtt ccttcgtcgt cgaacgagag
      781 ctcgcgcgtc tcgatgaacg actgcttctc cgcgggcttc ttgcgctcct cggcgaagcg
      841 cgacgcgggt ttctcgccct gcggcttggg cgacgacggg cgcggagccg aggacgagcg
      901 ctcgaggccg tccgcttttg cgtcgcgacg caggctgaat tcattgtgat cgaagttctg
      961 gaagtcgccc tcttcggcga tcgcctcgac cacctcgggc gccatgtgct tgcgacacag
     1021 ctcctcgaga ccggggtgcg aggtgcggtg cttgtcggcc aggccgacca cggagtggac
     1081 gtcctgcggc gacgacgtca ccacgatgtt caagagctcg accgcgcgcg ggaagtcctt
     1141 gagttcgagc gccagcgagg cgtggaacag catgacctcg gtcgtgaaga ggccgtcctt
     1201 cgatctctcc tgcaggcgcg agtagacttc cggcgtacag ttgccgcgct ggtggagagg
     1261 acggaggatc tccatcgcga ccgcggactg atcgagcccc agggctgcgt cgagtgccac
     1321 ccacacgcac tcgttgacgc cgtgcgcggg gtcgatgtag ggcttgagca gcatgatcac
     1381 ttcgcggccg gtgtccttgt tgtcggccat ataaccgcgc agcaccgggg ccgcggcgtc
     1441 gaagtcaccc ttgagcagga ggacttccgc cagcgcgcgc tccaccttgg gcgggaactt
     1501 ggtcttgtcg cgaagcgacg tcaagcggtc cacgcacagg ggaatcgcgg tcgaatcgta
     1561 acgggcggcc tcgacgatgc gtgcgatggc gtcgttctcg ttgccgttcg accacagcgc
     1621 gcacgcgtgc gcgaagcgga cccgtcccac cttggcgtgc ttcgactcga ggccggccag
     1681 gaagggcagc agcatcttgg tctcgaccgg cttctcgtcg atgatctcga ccagcgcggc
     1741 catgccctct ttcatgctgc cgagccgcac cgacaggagc gccgagagca ggtcgttggt
     1801 gaagatctcg gcgcgcagcg aatagccgcc gtggctggcc gacttgagcg attgcttctt
     1861 ggtcttggtg cgcgcgagca ggtcgcgcgc gacgtgatcc ggaatctgct cgaggtgctc
     1921 gagcgcggca tcgaggtcgc gacgcttgac ggcgttctcg accacgtagc gcgcgagtgc
     1981 ggggtcgtgc accgccagca acttgtcgcg cacgaactgc ccgacgcggt ccttgtcctt
     2041 ggtgaatttt tcctgcgcgg tcttgaggaa gttggtggcc tgcatgacct cgccgccctc
     2101 gaggcaggcg tcgaccgcgt cgaggtacag gtccagctcg tcggggaact gggcgatggt
     2161 ctgctccaag cgcttgacgg ccttgtcgaa gtggccgcgc ttgcgctcct gcatggcctt
     2221 ttgctgggcg gatgccgctt tttcgttcat gcgatggtca cggttggggg gatcgacggc
     2281 gaacggtcac tcatagatag cgctctaagc gaatcgcata ccaagaaatc gcgcgcgcca
     2341 gcgcgcgcaa aatataatgt aagtcattgt aaaagaaata gatatggctc gctcgaagcg
     2401 ccgacgccca ctcgatcgcg gtccctgacc ggctgcaaat tgcacacgcc aaccggcgcc
     2461 cattgcaccg atcctgatcg ttcaagtatc attcatgccg acagttgcgt atccccgcgt
     2521 ggttgtttac gactccgccg cggtccgctc tcccgatatg accggaacct tcaccctgtc
     2581 cgtccagtgc cggctggcgt cctcgcacgt gcttgcggga tgcccgccct gcgaccgctt
     2641 gcacggccac acctggaccg tgcgcgcgta ctgggagttc gccgccctcg acgagcacgg
     2701 gatgggagcc aattttcgcg acctcaaggc ggtgctgcgc cgcgaagtcc acgaccgctt
     2761 cgaccaccgg catctgaacg acatcgcgcc cttcgaccgc gttcccccca cggccgagaa
     2821 tctcgcgcgc gaggtgttca cgatcctgcg cgagaggttc aaccccggcc cgcgcggcgc
     2881 gctcgcgcgc gtcgaggtgt gggaaggccc cgaggcgtgc gcggcctacg cggagtgaga
     2941 tggtcctcgt gaccaaaccc agatcgatcg cgctggtcag cggcggcctc gactcgctgg
     3001 tgtcgctcgc acgcgcggtg gtcgagcgcg acgtgcgcat cgtgatgttc ttcgactacg
     3061 ggcagcgcgc gcgcgcgagc gagcgcgtct ccgcgatgag tgcggccaac tactacggcc
     3121 tcccgttcct ggacgccgac gtgcgctggc tggaatcgct ttcgccctcc gggatgcgct
     3181 cgtcggaccc ggactccgag gccggcctcg ccaccctcga cgacgtctgg atccccaacc
     3241 gcaatggggt gtttatcaac gtggcggccg cctacgccga gagctacggc tgcgacacgg
     3301 tcgtgaccgg attcaatcgc gaagaggcgg aggaatttcc cgacaactcg cgcgagtatg
     3361 tcgaacgggt gaacgccgcg ctggccctgt cgacgcgcaa ccgcgtgcgc gtggagagct
     3421 tcaccatcga cctggacaag cgcgcgatca ttcggatggg gctcgagtcg aaggcgccgc
     3481 tctcgatcgt gtggagctgc taccgctcgg gcccgctcat gtgcgggcgg tgtgcgtcgt
     3541 gcgcgcggct gcgcggcgca atcgattcac tgccgccggc ggagcgcccc gtgatcgaat
     3601 ttgcagacgt atgaccttcg cttatcgcat catcgaagga gagaccgcgt acaccggcgt
     3661 ggagctgcgc agcgggtggg tggcggagcg gaccggcctt tccggcgacg cggcggccgg
     3721 attcgtgggg aagtgccgcg tgcccaacga gaacctggtc gacttggacg acgcgcgcgc
     3781 gggctttttc atcgcggccg cgtcgatggc gcacgtgatc gcggagcacg cgtgtacgct
     3841 ggaggtcgcg gtgctgcgcc agcgattgct ggtcgccctc ctgggcgaaa tcctgcgcaa
     3901 acgcggaaag cgcatgcgcc gcgagggcga cgacttgtat tatgatgacc gcaagctgac
     3961 ggtctcgatc gcggcgccct cgtcggccac gtgcctgatc cacctcggga tcaacatcga
     4021 cccggccggc gcgccggtcc cggcggtggg attgaccgag atgcggctgg atccccgcga
     4081 gatcctgaac gaattgctcg cgaactacca agacgagctg gcgagtgtcg cgcacgcgac
     4141 caccaaagta cgaacggtgc cctgatgtcg acgaattccg aacagaatca cacggccgac
     4201 gcgcgcgaga tcgcgccggc gtcgaccgcg gccgcgggtt acctctcgga gatgttctgc
     4261 tccgtccagg gcgaggggct cttcgtcggc gagcggcaga tcttcgtgcg cacggccggc
     4321 tgcgccgcca cctgctcgtg gtgcgatacg gtctacagca aggtgcagac gccgcgcttt
     4381 gtcgtgcacg gcgagggtga gacgcccgcg cgctggcgcg cgaacccggt ggcgctcgag
     4441 gaggtcttgg acgatgtggt cgcgtgcgcg cgcgcgaacg cccccgtcgt cacggtcagc
     4501 gttacgggcg gcgagccgct ggagcagccc gagttcgtcg cggcgctctc gcgcgggctg
     4561 cgcgaacggg gcttccgcgt ctatttggag acggccggca tccacccgga cgcactgcac
     4621 acggtcgtcg atcacgtcga cgtgattgcg atggatatca agcttccttc ggcgacgggt
     4681 gttgcacact gggacgcgca ccgcgagttt ttctcggtgt tgcgagggag cgagttcgac
     4741 cccgcgcgcg gagcgcgcgc gtgtttcgtc aaggtgatcg tcgatttgaa ggcgacggtc
     4801 gcagagatcg agcgcgcggc cgagttggtc gcgtcgttca gcccgcgcgt tcccttcgtt
     4861 ctccaacccg aaagcgagac catgctctcc aagaacgcca cgcgcgagaa tcgtcgcgcg
     4921 ctgctcgaat tgatcgatct cgccgcgcgc gcagcgagcc tgcgcctgga ttccgtgcgc
     4981 gtcatcccgc agacgcacaa ggtattgaac gtgcgatgaa cgccgatttt ctcaccgccg
     5041 ccgtcggcct ggtggcgttc atcttttccg tgatcgtgca cgagaacgcg cacgggctgg
     5101 ccgccgagcg cttcggcgac cccaccgcgc gcgatctggg ccgcatcacg atgaacccga
     5161 tcccgcacat cgatcccatc ggtagcattc tcttgccgct ggccgcgttt ctctccggga
     5221 ttccgtttct cggctgggcg aaaccggtgc cggtcaatgg cgcgaatctt cgcaatccgg
     5281 tggtgcacag cgcgtacgtg gccgcggccg gtcccgcgtc gaacttcctg ctcgcgttcg
     5341 ccgccgcctt cttgtggatc gcggtcggtc tggtgttcaa gcacgtcccg ggcttgatgg
     5401 agacggggga gcgatcgctc tacttcttca atacgctctg cagcagcttg attctcatca
     5461 actgcgtgct ggcgatgttc aatctcctgc ccatcccgcc cctcgacggg cactggatcc
     5521 tgatgcgcta cctcccgccc gggccgcgcg aagcgctggc gtcggtgggg cgctggggct
     5581 tcttcatctt gattctgctg ttgtggagcg gtgtgctgtg gcgcatcttg ggaccgccgc
     5641 tctcgctggt cgtctcgggg tatcacgccc tggtcacgac ggtggtgcgg gcgttgtaga
     5701 aaaagcgaag ccctcccggt ttgcaccgag agggcttctg ttcacatccc tacgcagtga
     5761 gaacctaatc gatctcgccc ggattgtcgc cgcggcgatc cgaaggcggc gcctcgcggt
     5821 tgcgaatcgt caccaccgcg aagcggctct ccgtgtcccg cgtgctcacc gacgtcaccc
     5881 ggtagtcgta ctcgcggccg aagcgcgcgc ccccgtcgac cacgctcgtg acttcgagcg
     5941 gggctgcatt caagcgctcg atcgagctct cgaggacgtc gacacgatag acgttgtagc
     6001 cgcgcacgtt gggctgcgag ctcgcgttcc acgatatctg gatgtcgccg ccgttgatga
     6061 cgacggcctg gaggttggtg ggcgcgagta ccggcgcttc gtcctcggac ggttggggcg
     6121 ccgccgcatc ttcggcacac ccgaacgcaa ccagggacac ggccagcaac gcgcacgcca
     6181 gcacgaggtg cttgcggatc gccatgacga ttccttccga acgcggccgc gcggaatgct
     6241 cccgcgcgcg tcgcactcta tgtgaatgag tcgctagctt gcttaggctg cggagcggtg
     6301 cggtgtactc gtacagcgga tgccatgtgc ggcgaccgcc ccgcagcacg gcgggctatt
     6361 cagcaaccca cgtgccgacc cgcgatcttc gcatggcgac gccgactttt ccgcacaccc
     6421 aagagcgcat ccggcgccgc cgccgcgcgc gcgtccgcgt tctgccgcgt ggtggcgcat
     6481 cgcgccgcgt ggtcctgcca gaatgagagg agcgcgttgc attaccggcg gcttaacggc
     6541 ggggcggcgg cgcgcgagcg gccgccggcc ggggcggaca aaaacccctt gacctgtcac
     6601 gagttatggg gcacaatggc ccgcacgttc ccgaaacgtt ttggcccccc gcgggattga
     6661 ttcactgggc ttttgcaatg cgtttgcaag gaggctcgcc cgatgaccaa agccgaaatc
     6721 gtcgaggaca tcgccagcaa gaccggcctc accaagaagg acgtcgccac ggcgatggac
     6781 atgttcctcg aatccatttc gaaggcgctc tcggaaggca aacactacga gattcgcggc
     6841 ttcggaacct tcaaggtcaa gaagcgtaag tcgcgcatgg cgcgcaaccc gcgcaccgga
     6901 gagtcggtgc cggtgcccga ccgcaaggtt ccggtgttca aggtgtcgaa ggaactcaag
     6961 gacctcgtcg ccaacgttta gaactccaaa tctctttgca tacgatggtg cgccgcgcgg
     7021 ctgtgaaccg cgcgggtatc gtcgtttgcg gtgatcccat gccggaagcc cgtcgtttca
     7081 tcgacttgcg cagcgacacg gtgacgcgtc ccaccgcggc catgcggcgc gccatcgctg
     7141 aggcggaagt cggcgacgac gtcttcggcg acgaccccac cgtgatcgcg ctcgagaagc
     7201 ggatcgcaac cctcctcaag aaagaatcgg cgctgttcgt cccctcgggg acgatgggga
     7261 acttcctcgg cgtcgcctcg ctcaccgaga ccggcgacga ggtgatcctc gagcgccagt
     7321 cgcacatcat gaactacgag gtctcgtcgg tggcggccct ccacggggtc cagctcaacg
     7381 cgctggacgg cgatcgcggc gtgctgacgg ccgcccagat cgagccctat ataaagacgc
     7441 agtcgctgca cacgcccggc accaaggtga tcgcgatgga gaacacgcac aaccgcgcgg
     7501 gcgggcgcgt ctatcccttc gacgagatgc agcgcgtcaa gaagctggcc gagacgcacg
     7561 gcatccgcgt ccacctggac ggcgcgcgcc tgtgcaacgc ccacgtcgag acgggcgtgt
     7621 cgctcgccga ctacgccgcg tgcgcggaca cggtcagcat gtgtttctcg aagggactgg
     7681 gcgcgccggt gggatcgatt ctggtatcga cacaggatgt cgtagaacgc gcccgccggc
     7741 tacgaaagcg actcggtggc ggaatgagac aagcggggct attggcggcg gccgccctcc
     7801 acgcgctcga ccaccatgtc gagcgcatgc acgaggacca caaaaacgcc aagcggctgg
     7861 cgtctttcgt cgagcaggtg cgcgggctct cgctcctctt tccggtggag accaacatcg
     7921 tggtggcgcg cgtcgacgag aaggtgttca cggtcgatgc gttgctcgcg aagttgcgcg
     7981 agcacggtgt gctcgccgtc ccgttcgggg ccggccgcgt gcgcatggtg acgcacctcg
     8041 acgtctcgag cgacgacatc gagcgcgccg gcgaggcgct cctcgcgctg ggtcgcggct
     8101 agccgccgcc gcgcatgggc gattcgcccg gcaccctcac gctcgtcgcc acgccgatcg
     8161 gcaacctggg cgatattacc taccgcgcca tcgaggttct aaagggcgcc gacgtggtcc
     8221 tggccgagga cacccgtcat acccgcattc tgctgaacca ttacggaatc gcgacccagc
     8281 ttctctccta tcacgaccac aacgaggcgc gcgtgacgcc gtcgatcatc gagcggctcc
     8341 ggcgaggcga gaacgtggcg ctggtgaccg acgcggggac gccggctatc tccgaccccg
     8401 gcttctacct ggtgcgcgcg gcgctggagg ccgggatcca catcaccatg gcgccgggcg
     8461 ccaacgcggt gctgccggca ctcgtgctct cggggtttcc gtgcgaagcc ttcgtgttcg
     8521 cgggcttcgc gccgcgtaag cccggcgagc tcaagcgcgc cgtcgaaacg ctcgagagcg
     8581 agccgcgcac gaccgtgttc tacgtctcgc cccaccaatt gcacaaggtg ctcgacgcgc
     8641 tggccgcgcg actgcccgaa cgatcgatcg cggtggcgcg cgagatgacc aaggtgcacg
     8701 aagaggtcgc gcgcggaacg gcggtcgacg tgcgcgacca cttcgcgaag tcggcgccac
     8761 gcggcgagtt cgtcgtcgtc gtcaagggca tcggcaaacg ccgccgcgtc gcggatgacg
     8821 acgccgaatc ggcgcaacct tgacggcttc gcgaaacggg tgatagggtc ccgcgtcatg
     8881 gacaaacgag ccctgcaagc gagcgcgcgc aagatcctcg aacccgtggt caacgtactg
     8941 gtcgcattcc acatctcgcc gctgctggta tcgatcttcg gtctcgcgtt cagcgtgtgg
     9001 ggcggcatcg tggtggcgcg cggctggttg ctcgcgggcg gcatcttcct cttgctcgcg
     9061 ggtctgtgcg acgtgctcga tggcgacgtc gcgcgccggc tcgggaaggc gtcgcgcttc
     9121 ggcgccttca tcgattcgat gctcgaccgc gtcagcgagt tcgtctactt cggcggcgtc
     9181 ttgatgtacg tggtgcgccg cccgagcggc ttcgaggtgt acgagcccgt cgtcattatg
     9241 ctggcgctga tgggatccgt gctcacgagc tacgcgcgcg cgcgcgccga gggcgtaggc
     9301 atcgagtgca aggtgggcgt gatggagcgc cccgagcgca tcgcgctctt ggcgctgggg
     9361 ttgattctcg gctaccgctt cctgatggcg gtcatgatcc tgctcgcggt gacgtcgctc
     9421 tacacggtct tccagcgcat ccgccacgtg caccgtgcgg cctccgattc cacccaccgg
     9481 acgtgaccgt ccagttgaca ctgcccgcct gatgtgataa agtcctgcac gcctcaaacg
     9541 ggacgccgcc cctgaacctg cctcgcttct cgaccgtcac cgcgtcccac catcgatccc
     9601 gcgcgaagca cgctctcaat ccacgcagtg ctttttctcc aacctacgga ggaagtcatt
     9661 ctccaaagag gaggctccca tggagctttc gagattgcgc gcttgggcgc agggactggc
     9721 gacggtcctc ttgctgtccg cggcggtcgc cggcgcccag cccgcgctgc cgcacgagaa
     9781 ccactacaac gtgtatcgca cgattccgct gacgtacacg agaccgatca cgctgatcga
     9841 tcagttcgga ccgatcgacg tgtcgctctt cgtgctggac aagttcgcca atcccgccga
     9901 gaagcggatc cccgagaccg gcgacgtgta cccgatcatc aatcccgaca tccatcagct
     9961 gtggtggagg atcgacgcgc cccagccggc ccgcaccatc gtgggcatcg atcagttcgg
    10021 catcgcccag tggcacctcg gggacgcggc gtacctcttg aacccggcgc tcaagaacgt
    10081 gccgccgccc gctgcacccc ccgagtggaa ccactacctg tgttaccacg cgcaaggccc
    10141 cacggtgggc aggccggtga tcgtgatcga tcagtttggg acgagcaacg tcgtcgtgtt
    10201 gtacgggaag ctgttctgca acccggtgga gaagcgccac gccgatggga tcacgtatcc
    10261 gatcgtcgac cccgtggcgc atctcgcgtg ctacctggtc cagaacgcaa cgcccttcac
    10321 gcggtcgatc acggccatcg atcagttcgg gtattggcag ttggagacgt tcgagaacga
    10381 ctgcctatgc ctcccggcgc tcaaggagca cgtcttgaag acccaggaat cgacttgggg
    10441 gaagatcaag gcgatgtatc agtagttctc gaatcgaacg tacccaccgt tcgagacgag
    10501 acggccggtc gctccgcccg agcgaccggc cgttttgctc actcccaatc gagtgcttcg
    10561 accagggccg cgtgcacgtc gtcgacgctg cgcgtgccat ccagcgtgac cacgcgcccg
    10621 aagtgggttt cgtagcgcgg caggagcgtc tcgtaattcc gcgcgatcgt ttcgagaagc
    10681 gcgcgcgtct cgtagatcgc ggccgtgcct tttcgcgaag aaagccgctg cagacacacc
    10741 tcgaccggca ccgatagaaa gatggtgagg tccgggaccg cgcagccctg gttgacgtcg
    10801 atcacccact ccgcggatat cgacgagtcg agcgtctgat aggccatcga cgaaaagacg
    10861 tagcggtcgc atatcacgtg ctcgcccgcg gcgaggtgcg acgcgatcgc gcgcgagtgc
    10921 gcgagacggt cggcggcgaa gagcaaaccc atcacgtctt cgcccggccg atagaccgag
    10981 ccgtcgctcg cgcgctcttg tcccgtcagc aaccgacgaa t
//
LOCUS       DASPGK010000422         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1273438, whole genome shotgun sequence.
ACCESSION   DASPGK010000422 DASPGK010000000
VERSION     DASPGK010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273438"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VEC56_11695"
     CDS             <1..316
                     /locus_tag="VEC56_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14859.1"
                     /translation="ATVAVISLLHVVLTARFYGEWSADALWFLAAGLGLLLLAVVNWA
                     HVGLEPCRLPTAPVVLWANVVYLLLGVAALVAVPQVQAVLLVAGLAAQAVAGRFTLRA
                     PA"
     gene            367..768
                     /locus_tag="VEC56_11700"
     CDS             367..768
                     /locus_tag="VEC56_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HXV14860.1"
                     /translation="MNAEPTPWYAIPILYAVGVLLIALAIPLMGRRIKPNGLYGIRFP
                     ATLEDESVWYDINALGGRHLAAIGVVYLVLLTVAVVFLKTWPDDRRFLVPAVFVVVAL
                     VADAIFLAIASSRLLAKRRASPGTRRREETP"
     gene            768..1157
                     /locus_tag="VEC56_11705"
     CDS             768..1157
                     /locus_tag="VEC56_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXV14861.1"
                     /translation="MHPDTRKYNQDLDAADRKICDLLAKEIDKGLPEAENKVWHRHPV
                     WFLDGNPVVGYSKLKDCIRLLFWSGQSFEEKGLAKEGSFKAAEARFTTADQVNAKDLA
                     RWLKKARDIQWDYKNIVKRKGRLERLK"
     gene            1240..1752
                     /locus_tag="VEC56_11710"
     CDS             1240..1752
                     /locus_tag="VEC56_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14862.1"
                     /translation="MRRSTLAVLFGLAVLFTTAFAQAADKDKVSIRYLPGYPLPDRSS
                     EWLEVRVERMGSPKGDEQEIDRFFASVAATLSENGVDGDWQVVIPDAPSIEITIDLDG
                     RQIRLASAHVSVERSGAYVVTEQGMVALDGRTLDAVLSQQSEEFRRHRLVFERLLELA
                     LDRTRAEFSP"
     gene            1848..>2784
                     /locus_tag="VEC56_11715"
     CDS             1848..>2784
                     /locus_tag="VEC56_11715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV14863.1"
                     /translation="MTLLSGTRLGPYEIVAPLGAGGMGEVYRARDTRLGRDVAVKVLP
                     QHLSSHPEVRARFEREAKTVSSLNHPHICTLFDVGRDGDVDYLVLELVEGETLAHRLE
                     KGALPVHEVLRLGRAIGEALERAHRAGVVHRDLKPGNIMLTKTGPKLMDFGLARSAGL
                     AGGVGSGSVRSNATQSPTVGAPLTAEGTIVGTFQYMSPEQLEGKEADARSDVWALGCV
                     LYEMATGARAFEGQSQASLIAAIMTGEPRAVRDLQPVAPADLEKVIKQCLQKNPEDRM
                     QSAHDLAFALELVGGADAGAAPGSVTTARRGVRWWH"
BASE COUNT          485 a          810 c          999 g          490 t
ORIGIN      
        1 tgcaaccgtc gcggtgatct cgctcctcca cgtggttctc acggcgcggt tctatgggga
       61 gtggtcggcc gacgcgctct ggttcctcgc ggccggattg ggcctgctgc tgctcgcggt
      121 ggtgaactgg gcgcacgtgg gtctggagcc atgtcggcta ccgacagcgc cggtggtact
      181 ctgggcgaac gtcgtctatc tgcttcttgg cgtcgcggcg cttgtcgcgg tcccgcaggt
      241 ccaggccgtg ctgctggtag cgggcctcgc agcccaggcg gtggcgggtc gattcacgct
      301 gcgcgctccc gcctaggagg cacacatgag aaaccccaaa gcgaagtgga aggcgctgat
      361 ggaggcatga acgcggaacc gactccgtgg tacgcgatcc cgattctcta cgcggtgggc
      421 gttctcttga ttgcgctcgc gatcccgctg atgggtcgcc gtatcaaacc gaacgggctc
      481 tacgggattc ggtttccggc cacgctggaa gatgaatcgg tatggtacga catcaacgcg
      541 ctgggaggac ggcacctggc cgcgatcggt gtggtctatc tggttctgct cacggtggcg
      601 gtggtcttct tgaagacatg gcccgacgat aggcgtttct tggtgccggc ggtttttgtc
      661 gtggtagcac ttgtggccga tgccatcttc ctggcgatcg cgtcgagtcg gctgctggcg
      721 aagcggcgtg cgtcgccggg cacgcggcgc agagaggaaa ccccgtgatt caccccgaca
      781 cccgcaaata caaccaggac ctcgacgcgg ccgatcgcaa gatctgcgac ctgctcgcga
      841 aggagatcga caagggtctg ccggaggcgg agaacaaggt gtggcaccgg catccggtgt
      901 ggttcctgga cggcaatccg gtggtgggct acagcaagct gaaggactgc atccgcctgc
      961 tgttctggag tggtcagtcg ttcgaggaga aaggcctcgc gaaggaaggc tcgttcaagg
     1021 cggcggaagc gcgtttcacg accgccgacc aagtgaatgc aaaagacctc gcgcggtggt
     1081 tgaagaaagc gcgcgacatc cagtgggact ataagaacat cgtcaaacga aaagggcgcc
     1141 tggagcgact caagtaagaa ggccaaggcg tcgttcgtcg agtcatcatg ctcgatgaag
     1201 gcgaatctct ctcgtcataa tgagctggag gtcgtgacga tgcgtcgttc tacattggca
     1261 gttctcttcg ggctcgcggt tctctttact accgctttcg cccaggccgc ggacaaggac
     1321 aaggtcagca tccgatattt gccgggttac ccgcttccgg accgcagcag cgagtggctc
     1381 gaggttcgcg ttgagcggat ggggtctccc aagggagatg agcaggagat cgatcgcttc
     1441 ttcgcctcgg tcgcggccac actctcggag aacggcgtcg acggtgactg gcaggtggtc
     1501 atcccggatg caccgtcgat cgagattacg atcgacttgg acgggcggca gatccggctc
     1561 gcctccgcgc acgtatcggt cgagcgaagc ggagcgtatg tggtaacgga gcagggcatg
     1621 gtagcgctgg atgggcgcac actcgacgcg gttctgtcgc aacagagcga ggagttccga
     1681 cggcatcggt tggtcttcga gcggctgctc gagctggcac tggatcgcac gcgagcggag
     1741 ttctctccat gagcgaggcc gtcgctgaat tggagcgtta ggcgcgagcc cccatattat
     1801 gaacccgcca tcgacacgct gccgcgcgac gcggtaggat gccccgggtg actctcctct
     1861 ccggcaccag acttggaccc tacgagatcg tcgcgccgct cggcgcgggc ggtatgggcg
     1921 aagtgtatcg ggcgcgggat acgcgcctgg gccgcgacgt cgcggtcaag gtactgccgc
     1981 aacatctgtc ctcacacccg gaagtgcgcg cgcgcttcga gcgcgaagcg aagacggtct
     2041 cctctctcaa tcatccgcac atctgcacgc tcttcgacgt gggccgggac ggcgacgtcg
     2101 actacttggt cctggaactg gttgagggcg agacgctggc ccaccggctg gaaaaggggg
     2161 cgctgccggt gcacgaggtg ctccggctgg ggcgcgcgat tggggaggcg ctcgagcgcg
     2221 cgcatcgggc gggtgtggtg caccgcgatc tcaaaccggg caacatcatg ctcacgaaga
     2281 cgggcccgaa gttgatggac ttcgggttgg cgcgcagcgc ggggctcgcg ggcggggtgg
     2341 ggagcggaag cgtacgcagc aacgccacgc aatcgcccac cgtgggagca ccgctcacgg
     2401 ccgagggcac gatcgtgggg acgttccagt acatgtcgcc cgagcagctg gagggcaaag
     2461 aggcggacgc gcgcagcgac gtgtgggcgc tgggctgtgt cctgtacgaa atggccacgg
     2521 gagcgcgcgc cttcgaggga cagagccaag cgagtctgat cgcggcgatc atgacgggcg
     2581 agccgcgcgc ggtgagggac ttgcagccgg tggcaccggc ggatctggag aaagtgatca
     2641 agcagtgcct gcagaagaac cccgaggatc gcatgcagtc ggcgcacgac ctcgccttcg
     2701 cgctcgagct ggtgggcggc gccgacgcgg gagcggcgcc ggggagtgtg acgacggcgc
     2761 ggcgcggtgt ccgctggtgg cacg
//
LOCUS       DASPGK010000423         4200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1507614, whole genome shotgun sequence.
ACCESSION   DASPGK010000423 DASPGK010000000
VERSION     DASPGK010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507614"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1645
                     /locus_tag="VEC56_11720"
     CDS             491..1645
                     /locus_tag="VEC56_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14864.1"
                     /translation="MLRLTSALAFALVLVSSPPALATIAKPVSGTALDVFLGETIEVL
                     IKNAAGCTFDHTTSSSNAGVVNIINASGSNETSVKVGFTGAKVGTTNVGISTTGSCPN
                     LTHNYALTVSADYGAIQKEFGAQVKSSLKDYKIDTKLAFTNYRTTLAGLGVDYRDGAI
                     TDVELFQGFHDAGTTLRQQYWGAGLSAYADVINFGSGQLFDASAQFGEAPAGMFAFGW
                     GAFDDFQDFVCGDFAKFHTTYGKESHKAIKGYARDGAPMLGQLVTLPPIVSGGPLYPE
                     QLGAIPMLAPLTGPLTITSLPATNMAGDDGRIAVSGIGDSAQTGNLDVTLSFYQNSTK
                     KSSWTLSPGITSGEWDTEFTGLSEGTYIMTVGYGGDLGKIELPIQVQWNF"
     gene            complement(1811..1888)
                     /locus_tag="VEC56_11725"
     tRNA            complement(1811..1888)
                     /locus_tag="VEC56_11725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1851..1853),aa:Arg,seq:ccg)
     gene            2104..3234
                     /locus_tag="VEC56_11730"
     CDS             2104..3234
                     /locus_tag="VEC56_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14865.1"
                     /translation="MRALTRLAFPFTASLFSLPLAAAAQPVSAGPDVVLECESPEGAE
                     HTLNGTAPVGEGIVNEWSTDPVVALENADTLTPTGTFPLGVTTVTLTSTPGASDPVSD
                     DATITVEDTQAPVVRVYPNPRYLWPPNHTLHVIETDVRVRDRCSGAGDEDFGVELIDV
                     RSNESDNGLGDGNTSNDIQGADLGSDDRSVMLRAERAGPGSGRIYTLVYRITDGSGNT
                     TDAEANVHVPHDFADLKDLIGRDDDDEEDMEAICRRPADAVDQLAEMFPGLGSANDEG
                     VCNDICRAWARSCDDIAKGSGRCVAGEEKALAVIAFAECKDSDDRRDIAECREAVKDE
                     LAAQKAELRVETDEARDRCANMGRRCANACDDMFDDAAFSLN"
     gene            complement(3245..3321)
                     /locus_tag="VEC56_11735"
     tRNA            complement(3245..3321)
                     /locus_tag="VEC56_11735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3285..3287),aa:Val,seq:cac)
     gene            complement(3338..>4200)
                     /locus_tag="VEC56_11740"
     CDS             complement(3338..>4200)
                     /locus_tag="VEC56_11740"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXV14866.1"
                     /translation="LILNKEGLDEKITQVLNIWTGRPDLRAWSEIVQRAKHPESEVRI
                     GMVGKYVELTESYKSLNEALYHGGFANRAGVKIEYFDSEKLDEASVLLGCDGILVPHG
                     FGSRGAEGKTLAVGYAREQRIPYLGICFGMHFSVIDFARNVAGLERANSVEVDPDTPH
                     PVIDLLPEQREVKDKGGSMRLGAWPCVLRPGTNAYRAYGQERISERHRHRYELNPAYR
                     ERLERAGLVIAGTSPDDKLVEVVEIEDHPWFVACQFHPEFASTPFRPHPLFVDFIAAA
                     KRQRERTGPR"
BASE COUNT          716 a         1397 c         1420 g          667 t
ORIGIN      
        1 gatctggacg acggagccct tctcgacgcc gtacggcgcg agcacgctgc tcgcgctcag
       61 cacctcaccg gtgacgttga acgtcttcgt cgcggcgtgt gccgagcccg ccgcggcgca
      121 gagcagcaga cacacgccga gcaaaatgca gcgccgaacc gtcatgcccc cattctatcc
      181 gagatgcgcc cgtgcgaaga ggcagaagtc tcgacaggcg cctgcgttgt gaccgtgtgc
      241 aaccgatcgg gccaaccgct ggattcgcaa acgggatccg ctccccgtgg gctccgcgta
      301 gaacgcagcc gtgagcgcgc gaccccaatg gctgctggtc cgcgccggcc cagcgcggcg
      361 ccattcctcc acgcctccgc gttgggaggt aaacgaacgc ttataattgg ccattgccga
      421 ggaggcggct ggctttgcct gtcgtccgcg ctgggccgat gaccccccat gacggcagga
      481 ggctctgatc atgcttcgcc ttacatccgc tctcgcattc gcgctcgtgc tcgtctcttc
      541 gccaccggcg cttgccacga tcgccaagcc ggtgagcggt accgcgctcg acgtcttcct
      601 cggggagacg atcgaggtgc tgatcaagaa cgcggccggt tgcacgtttg accacacgac
      661 gagctcgtcg aacgccggcg tcgtcaacat catcaacgcg tcgggctcga acgagacatc
      721 cgtgaaggtc ggcttcacgg gcgcaaaggt gggcacgacc aacgtcggca tctcgacgac
      781 cggcagctgc cccaacttga cacataacta cgcgctcacc gtgagcgcgg actacggcgc
      841 gatccagaag gagttcggcg cgcaagtgaa gtcttcgctc aaggactaca agatcgatac
      901 gaagctcgcc ttcacgaact atcgaacgac gctcgcaggc ctgggcgtcg actaccgcga
      961 cggcgcgatc accgacgtcg agctcttcca gggattccac gacgccggca ccacgctgcg
     1021 tcagcagtac tggggcgcgg ggctctcggc gtatgccgac gtcatcaact tcggctcggg
     1081 ccagctcttc gatgcgagcg cgcagttcgg cgaagcgccg gctggcatgt tcgcgttcgg
     1141 ctggggtgcg ttcgacgact tccaggactt cgtgtgcggc gacttcgcca agttccacac
     1201 gacctacggc aaggagtcgc acaaggcgat caaggggtac gcccgggacg gtgcgccgat
     1261 gctcggccag ctcgtcacgc tgccgccgat cgtctcgggc gggccgcttt atcccgaaca
     1321 gctaggcgca attcccatgc tggcgccgct gacgggcccg ttgacgatca cctcgctgcc
     1381 cgcgaccaac atggctggcg acgacggacg catcgcggtg agcggcatcg gcgacagcgc
     1441 acagacgggc aatctcgacg tgacgctgag cttctaccag aacagcacga agaagtcgtc
     1501 gtggacgctg tcgcccggga tcacctccgg cgagtgggat acggagttca cgggcttgtc
     1561 cgaaggcacg tacatcatga ccgtcgggta cggtggcgac ctcggcaaga tcgagctgcc
     1621 gatccaggtg caatggaact tctgaagcga agccgccaat ctggctttgg cgatcggatc
     1681 cgtgtgcaac gcgggaaatg gtgacccggg cgcgatccgc gcggtccgct tcgcggacct
     1741 tgggcgaatc gcgcctgctg agttcgcaag ccgtgacttc ggcaatcgcg ctgcgatgca
     1801 agagtcgacg tggtgacccg ggcgcgattc gaacgcgcga cccccagctt cggaggctgg
     1861 tgctctatcc aggctgagct accgggccag cggtgccgtg gagagccctg tggctctcga
     1921 ggtttctctt ttcatctcgc gggcttgcgc gcgcgaacgg cagcgcgact gtagcgccga
     1981 tcgcgtgcgg cgctagctcg cgcgacgagg cgctccaccg tcggaagtcg cgtgaaacgg
     2041 ggttccagtg gcgcttgagc atgatacgat aaccccgcgt tccaccgtcg gaggtagctt
     2101 ctcatgcgcg cgctgactcg gctggccttc ccgttcaccg cctctctgtt ctcgctaccg
     2161 ctcgcagccg ccgcgcagcc ggtgagcgcg gggcccgacg tcgtgctcga gtgcgagtcg
     2221 cccgaaggcg cggagcacac gctcaatggc accgcgccgg tcggcgaggg gatcgtcaac
     2281 gagtggtcga cggatccggt ggtcgcgctc gagaacgcgg acacgctcac gccgaccggc
     2341 acgtttccgc tcggcgtgac gaccgtgacg ctgacctcga cgccgggcgc gagcgacccc
     2401 gtgagcgacg acgcgaccat caccgtcgag gacacgcagg cgcccgtggt tcgcgtctat
     2461 ccgaatccgc gctacctgtg gccgccgaat catacgctgc atgtgatcga gaccgacgtg
     2521 cgcgtgcggg atcgttgcag cggcgccgga gacgaagatt tcggggtgga gctgatcgac
     2581 gtccgcagca acgagagcga caacggcctc ggcgacggca acacgtcgaa cgacatccag
     2641 ggcgccgacc tcggcagcga cgaccgcagc gtgatgctcc gcgccgagcg cgcggggccc
     2701 ggcagcggac gcatctacac gctcgtctac cgcatcaccg acggcagcgg caacaccacc
     2761 gacgccgaag ccaacgtcca cgtgccgcac gacttcgcgg acctgaagga tctgatcggt
     2821 cgcgacgatg acgacgaaga ggacatggag gcgatctgcc ggcgccccgc ggacgccgtc
     2881 gaccagctcg cggagatgtt tcccgggctc gggagcgcga acgacgaggg agtctgcaac
     2941 gacatctgcc gcgcctgggc gcgcagctgc gacgacattg cgaagggcag cgggcgctgc
     3001 gtggccggcg aggagaaggc gcttgccgtg atcgcgttcg cagagtgcaa ggactccgac
     3061 gaccgccgcg acatcgcgga gtgtcgagag gccgtcaagg acgagctcgc cgcccagaag
     3121 gccgagctgc gcgtcgaaac ggacgaggcg cgcgatcgct gcgcgaacat ggggcgacgc
     3181 tgcgcgaacg cgtgcgacga catgttcgac gacgctgcgt tctcactcaa ctgattcgga
     3241 atcctggtgg gacgtagagg gatcgaacct ccgacctccg cggtgtgagc acggcgctct
     3301 agccagctga gctaacgtcc caggctcccc agtgtactca acgcgggccc gtgcgctcgc
     3361 gctgccgctt cgcggccgcg atgaagtcga cgaacagcgg gtgcgggcgg aacggcgtcg
     3421 acgcgaactc cgggtggaac tggcaggcga cgaaccacgg gtggtcctcg atctcgacca
     3481 cctcgacgag cttgtcgtcg ggtgaggtgc ctgcgatcac gaggcccgcg cgctcgagcc
     3541 gctcgcggta ggcggggttg agctcgtagc ggtggcggtg gcgctcggag atgcgctcct
     3601 gcccgtaggc gcggtaggcg ttggtgccgg gcctgagcac gcacggccac gcgccgagcc
     3661 gcatcgagcc gcccttgtcc ttcacctcgc gctgctcggg gagcaggtcg atcacggggt
     3721 gcggggtgtc ggggtcgacc tcgaccgagt tcgcgcgctc gaggcccgcg acgttgcgtg
     3781 cgaagtcgat caccgagaag tgcatgccga agcagatgcc gaggtacggg atgcgctgct
     3841 cgcgcgcgta gccgaccgcg agcgtcttac cctccgcacc gcgcgagccg aagccgtgcg
     3901 gcacgaggat gccgtcgcag cccagcagca cgctcgcctc gtcgagcttc tcggagtcga
     3961 agtactcgat cttcacgccc gcgcggtttg cgaagccgcc gtgatagagc gcctcgttga
     4021 ggctcttgta cgactcggtg agctcgacgt acttgccgac catgccgatc cgaacctccg
     4081 actcggggtg cttggcgcgc tgcacgatct cgctccaggc gcgcaggtcc gggcggcccg
     4141 tccagatgtt gagcacctgc gtgatcttct cgtcgaggcc ttccttgttg aggatcagcg
//
LOCUS       DASPGK010000424         6325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1273932, whole genome shotgun sequence.
ACCESSION   DASPGK010000424 DASPGK010000000
VERSION     DASPGK010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273932"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VEC56_11745"
     CDS             <1..570
                     /locus_tag="VEC56_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV14867.1"
                     /translation="HLVTRDVDLLASLAEHDAANVYVSVTTLDATLARTMEPRTSSPE
                     RRLDAIATLARAGVPTGVLVAPIIPALTDSEIPRILQRAAEAGATAAGHVVLRLPHGV
                     KEIFEEWLATHYPDRRDKILARVRALRDGKLYQSQFGERMSGTGIFAEQIHALFEASR
                     KKYDLARRSPGPSTAAFRRPPDPQGKLFE"
     gene            complement(579..1229)
                     /gene="can"
                     /locus_tag="VEC56_11750"
     CDS             complement(579..1229)
                     /gene="can"
                     /locus_tag="VEC56_11750"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HXV14868.1"
                     /translation="MAALDHLFANNRAWAARQVAREPDFFARLVAQQAPEYLWIGCSD
                     SRVPANEIVGLLPGELFVHRNVANVVVHTDLNCLSVLQFAVDVLHVKHVIVCGHYGCG
                     GVRAALATERLGLIDNWLRHVQDMCERHASLLDGISDPEARVDRMCELNVIDQVRHVC
                     ETTIVQDAWTRGQPLTVHGWIYGLNDGRLRDLRISVAALPSMADAYTTALRGLGAR"
     gene            complement(1243..2430)
                     /gene="fabV"
                     /locus_tag="VEC56_11755"
     CDS             complement(1243..2430)
                     /gene="fabV"
                     /locus_tag="VEC56_11755"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HXV14869.1"
                     /translation="MIIKPKIRGFVCITTHPVGCDANVRAQIDVVRARPRLHNGPKRA
                     LVIGASTGYGLASRIGAAFGSGAATVGVFLEREPAENRPATAGWYNSAAFEKHARAAG
                     LAARSLNVDAFSSAAKEKTIEVLRAIGPVDLVVYSLAAPRRTLASGEAAKSVLKPIGA
                     PYHGKTVDTDKALVTDVTIEPAIPQEIADTVTVMGGDDWEAWMDALAGAKLLADGCKS
                     VAYTYVGPQLTWPIYRDGTIGKAKEDLERAGRAIDDRLRAKGGAAYVSVMKAVVTQAS
                     SAIPVVPLYISLLFKIMKTKGIHEGCIEQARRMFEERLYSASAPAVDDHGRLRLDDWE
                     MREDVQAEVDRTWPRLTTENLHQLTDFKGYQEEFLRLFGFGIAGVDYDADVNPVVPFE
                     AVS"
     gene            complement(2434..3471)
                     /locus_tag="VEC56_11760"
     CDS             complement(2434..3471)
                     /locus_tag="VEC56_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HXV14870.1"
                     /translation="MSKRRVVLLPGDGIGPEVVEAARRVVEASGPEIEWVPREIGAVA
                     FEKYGEALPPDVLDEIRRCGVALKGPTTTPIGTGHISANVRLRKELALFANVRPVKTV
                     PGLETPYGQTDLVIVRENTEDLYAGLENEVAPGVITSIKVITETASRRIAKFAFEYAV
                     NWGRKRVTAVHKANIMKKADGLFLDCARLEAKAYQGRVQFDDILVDALAMKLVTKPSQ
                     FDVLLLENLYGDIVSDLAAGLIGGLGLAPGANYGPECAVFEAIHGSALDIAGKGIANP
                     IAMILSAAMMCHHLGDDASYLRIRKAVRTITTERRDVLTPDLGGQGTTRGVADAIVSE
                     VERRRREPVDA"
     gene            complement(3599..4108)
                     /locus_tag="VEC56_11765"
     CDS             complement(3599..4108)
                     /locus_tag="VEC56_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV14871.1"
                     /translation="MPPAIDADVRALARLLVDAVESGAAVSFVAPLTPARAEAWWRAT
                     LSESHARAVFLVARSEGAIVGTVQLHPAWAPNQPHRAEIVKLIVHRESRGAGLGARLM
                     QAAEDEARREGFRLLTLDAKRGAAAERLYRRLGWSYVGAIPRFAVDTDGVTPHDAVIF
                     YKELSPTAE"
     gene            complement(4286..5269)
                     /locus_tag="VEC56_11770"
     CDS             complement(4286..5269)
                     /locus_tag="VEC56_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIM domain-containing protein"
                     /protein_id="HXV14872.1"
                     /translation="MRTRIALLVGLVLVIACTAFAAESPTCAECGKPIERDAFQTQGR
                     VYHPDCFVCEICERPINGSYSEQKGKVYHNDCFLDRVALKCSLCNDVLRGEYLQDYWG
                     NSYCKHHDGDAPMCDSCGRFVSEKLTGGGTRYDDGRFICNICKPESMTDVDDILEIVF
                     EVAEHMKTFGMEVDYNGIQIHLVGRDKMQTLSGHHSNGLRGFTDYREDWRVFGRSQNR
                     KMHIYLLYGMPRMEIINTVAHELAHVWQFNRARFNNERAWAEGSCNYAAYLVLGKYPG
                     KESSFFRTSLTRDIDPVYGDGFRRVKTLAEAEGSKSWLRYLAKSADFPKGY"
     gene            complement(5315..>6325)
                     /locus_tag="VEC56_11775"
     CDS             complement(5315..>6325)
                     /locus_tag="VEC56_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV14873.1"
                     /translation="RNREGTLTALDPAPRWVVWPGRGTVALGRTLSDAVVARDIVAHT
                     VPAIARAELLGGWKALPEEDVFDVEYWDLEQAKLKRASAPAEHTGRVALVTGAASGIG
                     RATVDAFLKAGACVAALDIAGAVLSAWNDARVSAFVCDVTDATALDYAVENTVRRFGG
                     IDAVVSNAGTFPKTTRIADMTDELWRKSFAVNLDAHRLLLQKTIPFLEAGVDPAVVFV
                     ASKNVPAPGKGAAAYSVAKAGLTQLARVAALELAASGIRVNVLHPNDVFDTGIWTPEV
                     LAARAKEYGLDVEAYKKKNLLGREVTSADVAALAVALAGRLFAATTGAQIPVDGGNDR
                     VV"
BASE COUNT         1002 a         2194 c         2076 g         1053 t
ORIGIN      
        1 catctcgtga cgcgcgacgt cgatctgttg gcctcactcg ccgagcacga tgccgcgaac
       61 gtctatgtct ccgtgaccac cctcgacgcc accctcgcgc gcaccatgga gccgcgcacg
      121 tcgtcgcccg aacggcggct cgacgcgatc gcgaccctcg cgcgcgcggg cgtgccgacc
      181 ggcgtgttgg tggctcccat catccccgcc ctcaccgact cggagattcc gcgcattctc
      241 cagcgcgccg cggaagccgg cgcaaccgcc gcgggccacg tcgtgctgcg gctcccgcac
      301 ggcgtcaaag agatcttcga agagtggctc gccacgcatt atcccgaccg ccgcgacaag
      361 atcctcgcgc gcgtgcgcgc gttgcgcgac ggcaagctct accaatcgca attcggagaa
      421 cgaatgagcg ggacggggat cttcgcggag cagatccacg cgctgttcga ggcaagtcgg
      481 aagaagtacg atctcgcgcg acgatcacct ggaccgtcga cggctgcgtt tcgacgcccg
      541 ccggacccgc aaggtaaact cttcgagtga cgggcgcgct agcgcgcgcc caacccgcgc
      601 aacgccgtcg tgtaggcgtc cgccatcgac ggcagcgccg cgaccgagat gcgcaagtcg
      661 cgcaggcgcc cgtcgttcag gccgtatatc cacccgtgca ccgtgagcgg ctgcccgcgc
      721 gtccacgcgt cctgcacgat cgtggtctcg cacacgtgcc gcacctggtc gatcacgttc
      781 aactcgcaca tgcggtccac gcgcgcctcg gggtcggaga tgccgtccag caacgacgcg
      841 tggcgctcgc acatgtcctg cacgtggcgc agccaattgt cgatcaggcc caggcgctcg
      901 gtcgcgagtg cggctcgcac accgccgcac ccgtagtgcc cgcacacgat cacgtgcttg
      961 acgtgcagca cgtcgaccgc gaattggagc accgagagac aattgaggtc ggtgtggacc
     1021 acgacgttgg ccacattgcg gtggacgaac aactcgccgg gcaagagccc gacgatctcg
     1081 ttggcgggaa cgcggctgtc ggagcagccg atccagaggt actccggcgc ttgttgcgcc
     1141 accaagcgcg cgaagaagtc gggctcgcgc gcgacctggc gcgcggccca ggcccggttg
     1201 ttcgcgaaga gatggtcgag cgcggccatg cgaaacgcct tcctaggaga cggcctcgaa
     1261 cgggaccacc ggattcacgt cggcgtcgta gtccacgccc gcgatcccga agccgaacag
     1321 ccgcaagaat tcctcttggt agcccttgaa gtcggtgagc tggtggagat tctcggtggt
     1381 caggcgcggc caggtgcgat cgacttccgc ctgcacgtcc tcgcgcatct cccaatcgtc
     1441 gaggcgaaga cgcccgtggt cgtcgacggc gggtgcggac gcggagtaga gccgctcttc
     1501 gaacatgcgc cgcgcctgct cgatgcaccc ttcgtggatt cccttcgtct tcatgatctt
     1561 gaacaagagc gagatgtaca gcggaaccac cggaatcgcg gagctggctt gcgtcaccac
     1621 cgccttcatc acggagacgt acgcggcgcc tcccttcgct cgcaggcggt cgtcgatggc
     1681 gcggcccgca cgctccaggt cttccttggc cttgccgata gtgccgtcgc ggtagatcgg
     1741 ccacgtgagc tgggggccca cgtaggtgta cgccacactc ttgcacccgt cggcgaggag
     1801 cttggcgccg gcgagcgcat ccatccacgc ttcccaatcg tcgccgccca tgaccgtgac
     1861 ggtatccgcg atctcttgcg ggatcgccgg ctcgatggtg acgtcggtga cgagtgcctt
     1921 gtcggtgtcg acggtcttgc cgtggtacgg cgcgccgatc ggcttcagca ccgatttcgc
     1981 cgcctcgccg cttgcgagcg tgcgccgcgg ggcggccagg ctgtacacga ccaggtccac
     2041 cggcccgatc gcgcgaagca cctcgattgt tttctccttg gcggcgctgg agaaagcgtc
     2101 gacgttcaag ctgcgcgcgg cgagtcccgc cgcgcgcgcg tgcttctcga aagcggccga
     2161 gttgtaccaa ccggccgtgg ccggccggtt ttccgccggc tcgcgctcca ggaacacgcc
     2221 cacggtggcg gcaccgctcc cgaacgcggc gccgatgcgc gacgcgagcc cgtagccggt
     2281 ggaagcgccg atgacgagcg cgcgcttggg tccgttgtgc aaacgcggtc gcgcgcgcac
     2341 gacgtcgatc tgtgcgcgca cgttggcgtc gcacccgacc gggtgggtcg tgatgcagac
     2401 gaagccgcga atcttgggtt tgatgatcat cgcttacgcg tccaccggct cgcggcggcg
     2461 ccgctcgact tcgctcacga tcgcgtcggc cactccgcgc gtcgtgccct ggccacccag
     2521 gtcgggggtg agcacgtcgc ggcgctcggt cgtgatggtg cggaccgcct tgcgaatgcg
     2581 cagataggac gcgtcgtctc ccaagtggtg acacatcatc gccgccgaga gaatcatcgc
     2641 gatcgggttc gcgatcccct tgcccgcgat gtcgagcgcc gagccgtgga tggcttcgaa
     2701 caccgcgcac tccgggccgt agttcgcgcc cggcgccaga cccagcccgc cgatcaggcc
     2761 cgccgccaag tcgctcacga tgtcgccgta gaggttctcg agcaagagca cgtcaaactg
     2821 cgacggcttg gtgacgagct tcatcgccag cgcgtcgacc agaatgtcgt cgaactggac
     2881 gcgcccctgg tacgccttcg cttccaggcg cgcgcaatcc aggaacagcc cgtcggcctt
     2941 cttcatgatg ttggccttgt gcacggcggt gacgcgcttg cggccccaat tcaccgcgta
     3001 ctcgaaggcg aacttggcga tgcggcggct cgcggtctcg gtgatgacct tgatcgacgt
     3061 gatcacgccg ggtgccactt cgttctcgag gcccgcgtac aggtcctcgg tgttctcgcg
     3121 cacgatgacg aggtcggtct gcccgtaggg cgtctccaga ccgggcaccg tcttgacggg
     3181 gcgaacgttg gcgaacagcg cgagctcctt gcgcaagcgc acgttcgcgg agatgtggcc
     3241 ggttccgatg ggcgtcgtgg tcggaccttt gagtgcaaca ccgcagcgtc gaatctcgtc
     3301 gagaacgtcg ggcgggagcg cctcgccgta cttttcgaac gcgaccgcgc cgatctccct
     3361 cggcacccac tcgatctcgg ggcccgaggc ttcgaccacg cgccgcgccg cttcgaccac
     3421 ttcgggaccg atgccatcgc ccggcaacag gacgacccgc cgcttgctca ttcgattcct
     3481 ttcacgattg ggaacacgga tgtcttcgca ggatccggcc gacggccgga tgacccgcat
     3541 ggtagcacat gccggcgagg gcgggaaagg cgtctaggtc gcggcgcgtc cgcgcgggtc
     3601 actccgccgt ggggctcagc tctttgtaga agatcaccgc atcgtgcggc gtcacgccgt
     3661 cggtgtcgac ggcgaagcgc gggatcgctc ccacgtacga ccatcccagc cggcgataca
     3721 accgttccgc ggcggcgccg cgtttcgcat ccagcgtcag caagcgaaac ccctcgcgcc
     3781 gcgcctcgtc ctcggcggcc tgcatgaggc gcgcgcccaa ccccgcaccc cggctttccc
     3841 gatgcacgat cagcttcacg atctcggccc ggtggggctg gttcggggcc caggccggat
     3901 gcagctgaac cgttcccacg atcgcgcctt ccgagcgggc gaccaggaag acggcgcgcg
     3961 cgtgcgactc ggagagtgtc gcgcgccacc acgcctccgc ccgcgccggc gtgagcggtg
     4021 caacgaagct caccgccgcc cccgattcga ccgcgtcgac caaaaggcgc gcgagcgcgc
     4081 gcacgtcggc gtcaatggcc ggtggcatca atctctcgat cgtgtgggtt cgctcgctca
     4141 tgctggtgca caacgcggac cgtggcgcgc ccgtactgcc gcccgtcagc gcatcggcga
     4201 actcgagtca gcggcgagcc taggggggaa gacgagcgca ggtcaactat ttcgggaatc
     4261 ggccgtggcc gcgcggcatc gcgggctagt agcccttcgg aaagtccgcg ctcttggcca
     4321 agtagcgcag ccaggacttg ctgccttccg cctccgcgag cgtcttcacg cggcggaagc
     4381 cgtcgccgta gacggggtcg atgtcgcgcg tcaggctggt gcgaaagaac gagctctcct
     4441 tgccgggata cttgcccagc acgaggtagg cggcgtagtt gcagctcccc tccgcccacg
     4501 cgcgctcgtt gttgaagcgg gcccggttga actgccacac gtgcgcgagc tcgtgcgcga
     4561 cggtgttgat gatctccatg cgcggcatgc cgtacaacaa atagatgtgc atcttgcggt
     4621 tctgcgacct cccgaacacg cgccagtctt cgcgatagtc ggtgaatccg cgcagtccgt
     4681 tcgaatggtg tcccgacagc gtctgcatct tgtcgcggcc caccaggtgg atctggatgc
     4741 cgttgtagtc gacctccatg ccgaaggtct tcatgtgctc ggccacctcg aacacgatct
     4801 cgaggatgtc gtcgacgtcg gtcatggact ccggcttgca gatattacag atgaagcggc
     4861 cgtcgtcgta gcgggtgccg ccgccggtga gcttctccga gacgaaacga ccgcacgagt
     4921 cgcacatggg ggcgtcgccg tcgtggtgct tgcagtacga gttcccccag taatcttgca
     4981 ggtactcacc gcgcagcacg tcgttgcaca gcgagcactt cagcgccacc cgatcgagga
     5041 agcagtcgtt gtgatagacc ttccccttct gctcgctgta ggagccgttg atggggcgct
     5101 cgcagatctc gcacacgaag cagtcggggt ggtatacgcg tccctgggtc tggaacgcgt
     5161 cgcgctcgat gggcttgccg cactcggcgc aggtgggaga ctcggccgca aacgccgtgc
     5221 aggcgatcac cagcacgagc ccaaccagca atgcgatgcg agtgcgcatg ttcctcatgc
     5281 taactgtcag caaggaacgt ggcaaggaac cgcgctacac gacgcggtcg tttcctccat
     5341 cgacgggaat ctgggcaccc gtggtcgctg cgaacaatcg cccggccaga gccaccgcca
     5401 gggccgccac gtcggccgaa gtcacttcgc ggcccagcag gttcttcttc ttataagcct
     5461 cgacgtcgag tccgtactcc ttcgcgcgcg cggccaacac ctcgggcgtc cagatgccgg
     5521 tgtcgaacac gtcgttgggg tgcaggacgt tcacgcgaat gcccgacgcg gccagctcca
     5581 gcgcggccac gcgcgcgagc tgggtcaggc ccgccttcgc gaccgagtac gcggcggccc
     5641 ccttgcccgg ggcgggcacg ttcttcgagg cgacgaatac caccgcgggg tcgacccccg
     5701 cttcgaggaa cggaatcgtc ttctggagca gcaggcggtg cgcgtccaag ttgaccgcga
     5761 agctctttcg ccacagctcg tcggtcatgt ccgcgatgcg ggtcgtcttg ggaaacgtgc
     5821 ccgcgttgct caccaccgcg tcgatgccgc cgaaacggcg cacggtgttc tcgaccgcgt
     5881 agtccagcgc ggtcgcgtcg gtgacgtcgc acacgaacgc ggacacgcgc gcgtcgttcc
     5941 acgccgacag aaccgcaccg gcgatgtcca gggcggcgac acacgcgccc gctttcaaga
     6001 aggcgtcgac ggtggcgcgg ccgatgccgc tcgcggcgcc ggtcacgagc gcgacgcgac
     6061 cggtgtgctc cgcgggggcg ctcgcgcgct tgagcttggc ttgctcgagg tcccagtact
     6121 cgacgtcgaa gacgtcctct tcggggagcg ccttccagcc ccccaacaac tccgcgcgcg
     6181 cgatcgcggg gacggtgtgc gcgacgatgt cgcgcgcgac gaccgcgtcc gagagcgttc
     6241 gtcccaacgc cacggtgccg cggcccggcc acaccaccca gcgcggcgcg ggatcgagtg
     6301 ccgtgagcgt gccttcccgg ttgcg
//
LOCUS       DASPGK010000425         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_339318, whole genome shotgun sequence.
ACCESSION   DASPGK010000425 DASPGK010000000
VERSION     DASPGK010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339318"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VEC56_11780"
     CDS             <1..1160
                     /locus_tag="VEC56_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV14874.1"
                     /translation="LNDDAADVLTKLYLAQEKNGDTSGCEDTRALITKLGLSAKLDAA
                     AGKSGKPVDRTVVTEDNLWTKKHSEGERIDIESTPRFAPPNQPTTAPASPQPAGAVSG
                     GPSERDQWLLRLARGVGGGPPKTKAPAPAAAPAASAPVVEKAKPAETPAPPSPPSFAA
                     TEAEDIVDDRLSEDTVATAVEPEEPEVEEDIEAAVEDQEPEAEATSTPADEGLDLDDD
                     LPPIPETPPPSAARAAAPSKAPAAAKEPGVHISALIDEDDSAVGGEEDYRSHYDLGMA
                     YIEMDLLSEAIREYQIASKSPQFQVKCLEMIGVCFLKQNQPQLAIRQLNKGLSLIGNE
                     SEESIGIKYNLGLAYEMIGDLDNARTHFEDVYVVDVTFREVADKISKLSGS"
     gene            1273..>2829
                     /locus_tag="VEC56_11785"
     CDS             1273..>2829
                     /locus_tag="VEC56_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14875.1"
                     /translation="MRVRLVIAFFLVAGSLVPHGAARTLGEPSAPDPGLAGRCGAARS
                     LAVPAAGAALAARPSSPYSIPSEHFVVHYSAESLEDYARGVSGFAEHAYRVLVDTLHH
                     LEPPSDEPAGGDERIDVYLRTPLELGGAYGTTVPDTQVRTPYLNSYSSWIEIVDTMTV
                     AHCAVVTAHEVYHVVQLGYDRFESLSLLEMFSTWFEDRAHDDANWHYIFLKNFFRRPD
                     RGLFIQNYTNVTWAIFLTERHGDAIMQETLRRSADTPGPNPRGAFDGALQSTAGTTLV
                     DEFIEFGTWNYFTSLRDDGAHYSEGAVYPAIRCQRRSDCYPFPRHVTDHPMHELAANY
                     FFFDGDGHRGRLRIRVEPEPAATSYLTVTTFRGTTRERTTTRYDPGAPPDSFLVDDWE
                     HCDSVLAVYQIDRGLPQDNTVAVSARYEYDPPPATPWILVLDRDGCRNPFDGEGDEFA
                     ARDGEESPIATALLAAGETVVVTDRLDSDLSACRAVFVVGGFGEEGVTLSPHEFGTLG
                     GYMDQGGDVYL"
BASE COUNT          489 a          985 c          923 g          432 t
ORIGIN      
        1 gcttgaacga cgacgcggcc gacgttctca ccaagctcta tctcgcgcag gaaaagaacg
       61 gcgacaccag cgggtgcgag gacacgcgcg cgctcatcac caagctcggc ctgtcggcca
      121 agctcgacgc ggcagcgggg aagtccggca agcccgtcga ccgcaccgtc gtcaccgaag
      181 acaatctctg gaccaagaaa cactcggaag gagagcgcat cgacatcgag tcgacgccgc
      241 gcttcgcgcc gccgaaccag cctacgaccg cgccggcttc accacagccc gccggcgcgg
      301 tctccggcgg cccctccgag cgcgaccagt ggctcctgcg actggctcgc ggtgtgggcg
      361 ggggaccgcc caagaccaag gcgcccgcgc ccgcggcagc tccggccgcg agcgcgccgg
      421 tcgtcgagaa ggcgaaaccg gccgagaccc cggcgccccc ttcgcccccg tcgttcgccg
      481 cgacggaagc ggaggacatc gtcgacgacc ggttgagcga ggacacggtg gcgaccgcgg
      541 tcgagccgga agagcccgag gtcgaggaag acatcgaggc ggcggtcgag gaccaggagc
      601 cggaagccga agcaacgagc acacccgccg acgagggact cgacctcgac gacgacttgc
      661 cgccgatccc ggagacgccg cccccaagtg ccgcgcgcgc agcggcgcca tcgaaggcgc
      721 cggcggcggc gaaggaaccc ggcgttcaca tttcggcgtt gatcgacgag gacgactcgg
      781 ctgtcggcgg cgaagaggac tatcgcagtc actacgacct cggcatggcc tacatcgaga
      841 tggacctcct ctcggaggcg atccgcgagt accagatcgc gtcgaagtcg ccgcagttcc
      901 aggtcaagtg cctggagatg atcggggtat gcttcctcaa gcagaaccag ccgcagctcg
      961 cgattcgcca gctcaataaa gggctgtcgc tcatcgggaa cgagagcgaa gagtcgatcg
     1021 gcatcaagta caacctcggg ctcgcgtacg agatgatcgg cgacctggac aacgcacgca
     1081 cgcacttcga ggacgtttac gtggtcgacg tcaccttccg cgaggtggcc gacaagatca
     1141 gcaagctctc cggttcgtag gctcctttcg tatcccccaa gttttggctt taccgcgtcg
     1201 cgcggcggcg cgtaccttca cccatacaaa ccgctgcccc ctaaggtgcg tttgcaccgg
     1261 cggtcgtacg gcatgcgtgt tcgactcgtc atcgcattct tcctggtggc cgggagtctc
     1321 gtgccccacg gcgcggcgcg gaccctcggg gagccatcgg cgccggaccc ggggttggca
     1381 ggccgctgcg gcgcggcgcg gtcgctggcg gttccggcgg cgggggccgc gttggccgcc
     1441 cgcccgtcgt ccccgtactc gattccttcc gagcacttcg tcgttcacta ctccgccgaa
     1501 tcgttggaag actacgcgcg cggcgtgtcc ggtttcgccg agcacgccta ccgggtgctg
     1561 gtcgataccc tccaccacct ggagcctccc tcggacgagc cggccggagg cgacgagcgc
     1621 atcgacgtct acctgcgcac cccgctcgag ctggggggcg cgtatgggac caccgtcccg
     1681 gacacccagg tgcgcacgcc ctatctcaat tcgtattcgt cgtggatcga aatcgtcgac
     1741 accatgacgg ttgcgcactg cgcggtcgta accgcgcacg aggtctacca cgtggtgcag
     1801 ctcggttacg atcgcttcga gagcttgagc ctgctcgaga tgttctcgac ctggttcgag
     1861 gatcgggcgc acgacgacgc caattggcat tacatcttcc tgaagaattt ctttcgccgc
     1921 cccgatcgcg ggctgttcat ccagaactac accaatgtca cgtgggccat ctttctcacc
     1981 gagcgccacg gcgatgccat catgcaagag acgctgcggc gcagcgcgga tacacccggg
     2041 cccaacccgc gcggggcctt cgacggcgcg ctgcagtcga ccgcgggaac cacgctggtc
     2101 gacgagttca tcgagttcgg gacctggaac tacttcacga gcctgcgcga cgacggcgcg
     2161 cactattcgg agggcgcggt ctatcccgcg atacggtgcc agcggcggag cgactgctac
     2221 cccttcccgc gccacgtcac ggaccacccc atgcacgagc tggcggcaaa ctatttcttc
     2281 ttcgacggcg acggacaccg cggacgcctg cgcattcgcg tcgagcccga gccggccgcg
     2341 acgtcgtatc tcaccgtcac gacgtttcgc gggacgacgc gcgagcgcac gaccacgcgc
     2401 tatgaccccg gcgcgccgcc cgactcgttc ctggtcgacg actgggagca ctgcgactcg
     2461 gtactggccg tctaccaaat cgaccgtggg ttgccccagg acaacacggt cgcggtatcc
     2521 gcgcgctacg agtacgaccc gccgccggcg acgccgtgga tcctcgtgct cgaccgcgac
     2581 ggctgccgca accccttcga cggcgagggc gacgagttcg ccgcgcgcga cggcgaagaa
     2641 tccccgatcg cgacggcgct cctcgcggcc ggcgagacgg tggtggtgac ggaccggctc
     2701 gattcggact tgagtgcctg ccgcgcggtg ttcgtggtgg gtggcttcgg cgaagagggg
     2761 gtgacactct ctccgcatga attcggaacc ttgggcggct acatggacca gggcggcgac
     2821 gtctacctg
//
LOCUS       DASPGK010000426        16959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1040020, whole genome shotgun sequence.
ACCESSION   DASPGK010000426 DASPGK010000000
VERSION     DASPGK010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 16959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16959
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040020"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2555)
                     /locus_tag="VEC56_11790"
     CDS             complement(<1..2555)
                     /locus_tag="VEC56_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV14876.1"
                     /translation="MSLASTCIQRPVLTIVLAIVIVVFGIIGYTFLGVREYPNVDPAV
                     ITVTTTYTGTNADVIDSQVTQPLEEEISSVPGIRTISSVSREGRSQITIEFDLAVDLE
                     TAANDVRDKVAGAVGDIPPDVDPPEVSKADADATPIVVLNVSSPSRDLLKLSEIAEKV
                     FKERLQTINGVSEVRIFGEKRYAMRLWMDPLKMAAYGVTPLDVRTALAQENVELPSGR
                     IEGNTMELPVRTLSRLETPEQFNGLILREESGRLLRFRDIGIAELGPENERSISKGLA
                     GPRVAVALVPQPGSNHIAITDEFYKRVEQIERDLPDDIVLGVAWDTTKYIRRSIHEVR
                     ETIFTAFLLVVAVIFLFLRDWRTTMIPVVAIPISLIGIFFVMFAAGFSINVLTLLGLV
                     LAIGLVVDDAIVMLENIYAKVEAGMSPLEAGFKGSREVFFAIIATTVALMSVFTPIIF
                     MQGLTGRLFKEFGVVIGGAVAISSFIALTLTPMLSAHLLKPHAKHSAFYRKTEPFFVR
                     VNDGYRRSLAGFMRRRWLGVAAIVLCAGLMWLFFTSLPRELAPLEDRSRMRMTATAPE
                     GTSFERMSGYMDSVIDLVRREVPEADVVLANTSGFAGGANAGNCTVTLIPPEERERSQ
                     QEIADALSRSVRNLNDARTFVTQEQTISVGGGIARFGLPVQYVIQAPTIDKLKEALPV
                     FQEAAAEHPAFNFVDVNLKFTKPELVIEIDRDRARTLGVSVSDVAQTLQLALSGSRYG
                     YFIKDGKQYQVIGQLARSDRNEPFDLSAIHVRNRDGQLIQLDNVIDTSESSNPPQLFR
                     YNRYVSATVSAGLQPGYTLGDGIAAMDQVADKVLDESFATDLAGAARDFSESSS"
     gene            complement(2552..3649)
                     /locus_tag="VEC56_11795"
     CDS             complement(2552..3649)
                     /locus_tag="VEC56_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV14877.1"
                     /translation="MKFATGRTSLVVAAIVVAALVLIKVGLLQGDSRQGGRAGGGPGG
                     AGGGVTAVRAVVLGTQSMTDRISTVGTILANEDVDVRSEISGRVEAISFAEGSRVAKG
                     QLLVKIADAELRAQTARAESRLAIAESDAERQRQLFEQSLTSQREFDTAMNEFNVAKA
                     EAALVRAQLDKTEIRAPFDGKVGLRFVSEGSYVSPATPITAIQDDTPVKLDFSVPERY
                     AGRLKKGDGIRFTVQGTSRVFEGTIYALEPSIDTATRTMRVRATSPNEDGALVPGAFA
                     DVEIVMQQRDTIAIPAYALIPELKGHRVFVYAGGKAEARSVEIGTRTDENVEIVRGLS
                     AGDTLITSAILQLRTGAPVTVAGIDSMGGGP"
     gene            complement(3756..4817)
                     /gene="nadA"
                     /locus_tag="VEC56_11800"
     CDS             complement(3756..4817)
                     /gene="nadA"
                     /locus_tag="VEC56_11800"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXV14878.1"
                     /translation="MIRGATIEETYRLMVDKLARVVPEAELRIKAELVFEINRLKEER
                     GAIILGHNYMEPALYHTVPDVVGDSLELSRKAARTDKDPIVFCGVRFMAETAKILNPN
                     KTVLLPAKKAGCSLAASITAEDVRELKRQYPGVPVVTYVNCYADVKAETDVCCTSSNA
                     ARVVEALGADCVIFLPDEYLAQNVARETGRQVIFPTREPKSSGARDAAPRNLHYHLIG
                     WHGRCEVHEKFTVADIVAVRKQFPDVAVLAHPECSAEVVQASDFSGSTSKMIEFVKKS
                     NAPRYLLLTECSMGDNIIAENPDKETVRLCSVRCPHMNEITLEDTRDALLHNRYVIEV
                     PEDIRVRAKRALDRMLEIG"
     gene            5008..6087
                     /locus_tag="VEC56_11805"
     CDS             5008..6087
                     /locus_tag="VEC56_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14879.1"
                     /translation="MHRPSPTRALFAAAAILIPATVDFCFAQPLAKPKPGDSPRGVLV
                     LDGSPVHDVGILRVHASNWGAIGSQPGSAGPFSEAPSAEWPSGSRVEYLYVAGIWVGA
                     LLNGIPAVSTAAFATEFRPSSDPRDIVYESHIGVAGGHRVPSANADDDSDGFLDEDPL
                     DGFDNDLDGQVDEDYAAISDQMLARRFRDDDPSVILPDHNPMGLFVREESYQFDDPGY
                     DDFVGFTFTITNDGAGTLRDLYVGVLMDGDVGHRDTPNYWEDDATGLLTRVVYHDPHP
                     FRIYRFPYWYDVDGDGGQADAYAGIVLLDHPVDPTGTTAPTDVDVHTVSYFSGSLPYE
                     EGGDPTNDFERYEVLSNGVIRQPPV"
     gene            6172..6882
                     /locus_tag="VEC56_11810"
     CDS             6172..6882
                     /locus_tag="VEC56_11810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14880.1"
                     /translation="MVTPRDDFTHVDRAAVAYHGLWFDLDDDPATGIDGKEHQEHWYL
                     PNDNPTPIAITRFEARALDPHRVKVEWEASSSDGIESFDLLRATSGDALRRVVPGTFG
                     PATSVFVDETVTPGGHYEYVVVARDVRGAEFISPRIGVTVPSVVLSLGQNSPNPFNES
                     TSFGITLPERADVDVSVYDVSGRRVATIASGVRDAGDYDLDWNGTDDAGRRVAAGVYF
                     YRLEAGGRTLTRKLVLTR"
     gene            complement(6879..8078)
                     /gene="ispG"
                     /locus_tag="VEC56_11815"
                     /gene_synonym="gcpE"
     CDS             complement(6879..8078)
                     /gene="ispG"
                     /locus_tag="VEC56_11815"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXV14881.1"
                     /translation="MRIEAREVPTPANGSIVSQTTYLATRQVMVGAVAVGGGAPISIQ
                     SMATTPTTDVQRTVDEIKRMEDAGVDIARVSVPDIESVRATEKIVRQIRVPLVADIHF
                     NYRMAVEVAGTGIAKLRINPGNIGSKERVAAVVAKAKEYKLPIRIGVNAGSLEKDLLM
                     KYGFPTPEAMVESAFRHIGVLEEHDFTDIIVSLKASHVPLALAAYRLFSRKSQYPRHI
                     GITESGPGLSGAVISSVGLGILLHEGIGDTMRVSLAADVVDEVRVAQKILQGLHIQSN
                     TPRIIACPTCARNRIDLFRIAEEVEKKTQHLRKPINIAVMGCAVNGPGEAAGADIGIA
                     AGDGEGLIYIDGKIARKVQEEDMVSELMKEIEARWGAGATEGSAAKLTASHRDGAVGR
                     WVEEKND"
     gene            complement(8569..9801)
                     /locus_tag="VEC56_11820"
     CDS             complement(8569..9801)
                     /locus_tag="VEC56_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXV14882.1"
                     /translation="MNQHTQESLDAARVALLFQALIRNAFDVIKVVDPQAKTVFVSPS
                     VERVLGYQPRELIGTNPLDFVHPDDLERATQALESVRQSGSVETTGPIRARHKDGSWR
                     TMEAVLQNLVDDVDVRGIVMNYRDISERHESENQLRLLEERFEKAFRNSPDSITITDI
                     ETGRIIEVNDGFERITGYRRSDIVGRTTLELGIWKDLEVRKHLADVVRRDGRVRDMFI
                     EFVARGGAVLSCLVSAEVIHLDGRPCFLAVTRDITDRVIADEKLRQTTEKLQQEHQEV
                     LRKNIAFQEVVDHIDEEKAKYRHEICTNIDNLLRPTMRKLQQDGRLSRRDIDVLVHGL
                     DEITGKHINDYRNNVSKLTPREMDILDMIKTGRSSKQIAEALGLSSQTVHKHRQSIRR
                     KLQLDHREINLAAYLRSH"
     gene            9976..11802
                     /gene="uvrC"
                     /locus_tag="VEC56_11825"
     CDS             9976..11802
                     /gene="uvrC"
                     /locus_tag="VEC56_11825"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXV14883.1"
                     /translation="MSDADSTNRLEEAAEKFPSDPGVYLFKDDVGRVLYVGKAQSLRV
                     RIRSYFRGGDERASIRYLMQRARSLEYIVTDTEQEAYILENNLIKKHRPRYNVVFKDD
                     KTYVNLRLNVDHPFPRLTVVRRPRRDGAVYFGPFASAGSVRQTLRTIGRIFPLRTCTD
                     ADFSSRTRPCLYFYIKRCPAPCVGKIDAQEYAETVKRVTMFLKGRGDELVKSLRDTME
                     RQSAERRYEEAARTRDQVFAIQRVVERQRITSPQRAERDVFAAYRRGERLVIQLLSVR
                     EGEVQDGETFVFGSALEATGEHLASFINQYYQARAVIPDEVLVPEEISDRASLEALLS
                     ERAKRRVEIVVPKRGQGATLLELARKNARVAFEEGRTERNRELLEDLQVILGLEHYPR
                     RIECVDISNIQGTKAVGSAVVFIDGEPAKAHYRRFRIRTVEGSDDTGMMREVLERRVT
                     RGLKEGDMPDLLIVDGGRGQLNVALDVLKRLGADTVAAIGIAKVRNEKNRKIRGQERV
                     YLPAIEEPLLLDRQSQALYLLERVRDEAHRFAIGYHRTLRAKAMGESKLDGIPGVGPT
                     LKQRLLARFGSVERIRKARLDELAAVPGVSVRKAQAVKDALG"
     gene            11958..13283
                     /locus_tag="VEC56_11830"
     CDS             11958..13283
                     /locus_tag="VEC56_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14884.1"
                     /translation="MESKRTLAVLLSRHMAQFAALSLVAVLAACDEETTVEPPAPPPQ
                     IQTTQSYSKSSTGLPNVEVFAMLTLSNGEFWIGTSQGIARYPSVTATQRIPGADGVIN
                     ELNGLPNPKVRDIVEMDGVVYVATWGGGIAIYDITAGTWESRTPADDTADDVHPQNVS
                     LGRFVAPGTGIIVQPGPNGILETVPQGDDRISGNNIIDGAGGNGIAETTAHRGLNGAI
                     ADLEPSPIEGKLYLATNDAISIYDPALDRFSSFTSLTREVVSAVGVRDTPGGIERWYG
                     PRVETVEDDPPPPMPAAGITVWRGVSTFYTYTKVNSGLPEPNVNAIYFDADAGPDILW
                     VATANSGVSRVQVGASTWTTYTALQGLPSNTVYSVVRAAAPGGGSTIWVATQNGVARL
                     RGDGTWQGYNTSGGLAADRVRRVYSDDGLRLWIGYIDAGAARLNASGAK"
     gene            complement(13300..14445)
                     /locus_tag="VEC56_11835"
     CDS             complement(13300..14445)
                     /locus_tag="VEC56_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXV14885.1"
                     /translation="MARASARPSTILLYAASDNDADSLYATRFFAPDPFVFLRTGAGR
                     RILVMSDLEIDRAKRQSNAHRVLSFARYAALAEKRWKRTPHAADTIAEVLRALRIGSV
                     TVPASFPSGVTERLRKRRIAVYVADGPLFPERARKSAAEVKAIRDAMRATEAGMQAAV
                     DTLRRARVRKGWVVLDGRRLTAEDLRRAADTTILAHGCLPAHTIVAPGRQGCDPHDEG
                     SGPIRAGVPIIVDIFPRAQKNGYFADITRTFVKGRASKTVRAMYDAVHDGQRLALRSI
                     RHGARVRDIHDAIHALFAARGFQSGKIDGRMQGFFHGTGHGLGLEIHEPPRVAANDAV
                     LEAGMVVTVEPGLYYWPHGGVRIEDTVLVTRRGITNLTRFPKFFEIP"
     gene            14509..15342
                     /locus_tag="VEC56_11840"
     CDS             14509..15342
                     /locus_tag="VEC56_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXV14886.1"
                     /translation="MNDRRIDLHMHSNFSDGVHAPSVLVDLAKWKGISAIALCDHDSL
                     DGYPELEASARAAGVEVITGVELSCEHAGRDLHVLGYGVDPKSSALQDSLRSFRASRE
                     QRGVRIVALLREQGVHIEMDSVLAKAGGGALGRPHIAEALLEAGYVNDHAEAFAKYIG
                     EGGPAYVEKYKMSPAEAVGSIHGAGGLAFVAHPGYYLDNGDSFGVLLDHGFDGVEVFH
                     PYHRASVIERLLGIARERGLLISGGSDFHGFAGRDNLGEPPVSYALLERIRDTLEARP
                     R"
     gene            15339..15791
                     /gene="bcp"
                     /locus_tag="VEC56_11845"
     CDS             15339..15791
                     /gene="bcp"
                     /locus_tag="VEC56_11845"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:15468..15470,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXV14887.1"
                     /translation="MIEEGKTAPDFALTADDGTKVTLSALRGKPVVLFFYPKADTPGU
                     TAEACDFRDEIAAFRSKKAVVLGVSKDSVKAQAKFRAKYELPFPLLSDPDAAVCNLYG
                     VIQDKSMYGKVVKGIERSTFVIDAGGRVAKIYRKVKVAGHARAVLDDL"
     gene            complement(15748..16485)
                     /locus_tag="VEC56_11850"
     CDS             complement(15748..16485)
                     /locus_tag="VEC56_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXV14888.1"
                     /translation="MAKSARSSPLPTVVVNMAVSLDGRITTRAREHVALGSERDRRLM
                     DELRTRADAVIVGAGTVRHDGHPIVLRYADLRARRAARRGPHPVNVVLSRALDLPLRS
                     RFFASKDTRRIVFTTTNAPKRRVRAFERVAEVIVLPGKDLSPRRVLSTLVRRGYRRVL
                     LEGGGEVHFAFAKAGLVGEVYVTVTPRLIGGKEAPSLLDGEGFLWKDHMRLRLVSVKR
                     AGEEVFLRYRVLASTNRPARRARVRRP"
     gene            complement(16500..>16959)
                     /locus_tag="VEC56_11855"
     CDS             complement(16500..>16959)
                     /locus_tag="VEC56_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV14889.1"
                     /translation="GYVVTRNAKVAKLLRLGNYTQTAGVTTFLQYAAAEAMNNVDESR
                     RAVATMVAEFQRRRDALHDGLKDLPGVAIDRPAGAFYMFPDFSEFIPRELSGPERNHY
                     VYELLMEHGIASVYGSCFGKHFGDNVRLSFSATPVPVIHDAVERFRRVFR"
BASE COUNT         2902 a         5463 c         5605 g         2989 t
ORIGIN      
        1 gaggaactct ccgaaaagtc gcgcgccgcg ccggccaggt cagtggcgaa cgactcgtcg
       61 agtaccttgt ccgcgacttg atccatggcc gcgatgccgt cgcccagggt gtacccaggc
      121 tggagaccgg ccgacaccgt cgcagatacg tagcggttgt agcggaacaa ctgcggtgga
      181 ttcgacgact ccgacgtgtc gatcacgttg tcgagctgga tcaactggcc gtcacggttg
      241 cggacgtgga ttgccgatag atcgaacggc tcgttgcgat cggagcgcgc cagttgtccg
      301 atgacttggt attgcttgcc gtctttgatg aagtagccgt agcgggaccc gctcaacgcg
      361 agctgcaagg tctgcgccac gtcggacacg gagacaccga gggtgcgcgc acgatcgcgg
      421 tcgatctcga tcacgagctc gggcttggtg aacttgaggt tcacgtcgac gaagttgaag
      481 gcggggtgct ccgcggccgc ttcctggaag accgggagcg cctccttgag tttgtcgatg
      541 gtgggagcct ggatcacgta ctgcacgggc aacccgaagc gcgcgatgcc gccgcccaca
      601 ctaatcgtct gctcctgggt cacgaacgtg cgcgcgtcgt tgaggttgcg aaccgatcgc
      661 gaaagcgcgt cggcgatctc ttgttgcgag cgctcgcgct cctcgggggg gatcagcgtc
      721 acggtgcagt tgccggcgtt ggcccctccc gcgaagccgg atgtgttggc gagcacgacg
      781 tcggcttcgg ggacttcgcg ccgcacgaga tcgatgaccg agtccatgta gcccgacata
      841 cgctcgaatg acgtcccctc gggagcggtg gccgtcatgc gcatgcggct gcggtcctcg
      901 agcggggcca gctcgcgcgg gagcgacgtg aagaagagcc acatcagtcc ggcacacaag
      961 acaatggccg cgacgccgag ccagcgccgg cgcatgaatc ccgccagcga gcgacgatat
     1021 ccgtcgttca cgcgcacgaa aaagggctcg gtcttccgat agaacgcact gtgcttcgcg
     1081 tggggcttga gcaggtgcgc cgacagcatg ggcgtcaagg tgagcgcgat gaacgaggag
     1141 atcgccacgg caccgccgat cacgaccccg aactccttga acagccgtcc ggtgagtccc
     1201 tgcatgaaga tgatgggcgt aaaaaccgac attagcgcga cggtggtggc gatgatggcg
     1261 aagaacacct cgcgtgaccc cttgaagccc gcttccagcg gcgacatgcc ggcctcgact
     1321 ttggcgtaga tgttttcgag catcacgatg gcgtcgtcca ccaccaggcc gatggcgagc
     1381 accaggccca agagcgtcag cacgttgatc gagaagccgg ccgcgaacat gacgaagaag
     1441 ataccgatca atgaaatggg aatcgcgacg acgggaatca tcgtggtacg ccagtcgcgc
     1501 aggaacaaga agatgaccgc gaccacgagt aggaaggcgg tgaagatggt ctcgcgcacc
     1561 tcgtggatcg acctgcgaat gtacttggtg gtatcccacg ccacgcccag cacgatatcg
     1621 tcggggaggt cgcgctcgat ctgctcgacg cgtttgtaga actcgtcggt gatcgcgatg
     1681 tggttcgacc cgggctgtgg aaccagcgcc accgcgaccc gcggcccggc cagccccttg
     1741 gatatcgaac gctcgttttc cgggcccagc tcggcgatgc cgatgtcgcg gaaccggagc
     1801 aagcgcccgg actcttcgcg caggatgaga ccgttgaact gctccggcgt ttccaggcgg
     1861 ctcagcgtgc gcaccggaag ctccatggtg ttgccttcga tgcgccccga cggcagctcg
     1921 acgttctctt gcgccagggc ggtacgcacg tcgagcggtg tcaccccgta ggccgccatc
     1981 ttgagcgggt ccatccacag gcgcatcgcg tagcgtttct cgccgaagat gcgcacttcg
     2041 ctgaccccgt tgatggtctg caggcgctcc ttgaagacct tctccgcgat ctccgagagc
     2101 ttcaacaagt cgcgcgaggg gctgctcacg ttcaagacga cgatcggggt ggcgtcggcg
     2161 tccgccttgg agacctcggg gggatcgacg tcgggtggga tgtccccgac cgcgcccgcc
     2221 accttgtcgc gcacgtcgtt ggcggccgtc tccaagtcga cggcgagatc gaattcgatc
     2281 gtgatctggc tgcggccctc gcggctgacc gagctgatgg tgcgaatccc cggcaccgag
     2341 ctgatctcct cttcgagggg ttgcgtgacc tgggagtcga tgacgtcggc gttggtgccg
     2401 gtataagtgg tggtgaccgt gatgacggcc gggtcgacgt tgggatactc gcgcacgccc
     2461 aggaaggtgt agccgatgat gccgaacacc acgatgacga tcgcgagcac gatcgtgagc
     2521 acgggacgct ggatgcatgt cgaagccaga ctcacgggcc gccccccatc gagtcgatac
     2581 ccgccaccgt caccggcgcg ccggtccgca gctgcaagat ggccgacgtg atgagcgtat
     2641 cgccggcgga aagaccgcgc acgatctcga cgttttcgtc ggtgcgggtc ccgatctcga
     2701 cgctgcgcgc ctccgcttta ccgcccgcgt acacgaacac gcggtgcccc ttgagctcgg
     2761 ggatcagcgc gtaggcggga atcgcgatgg tgtcgcgctg ttgcatgacg atctcgacgt
     2821 cggcgaacgc gcccggcacc agggcaccgt cttcgttggg actggtcgcg cgcacgcgca
     2881 tggtgcgcgt ggcggtgtcg atggaaggct cgagcgcgta aatggtgcct tcgaacacgc
     2941 gcgaggtgcc ctgcaccgtg aaacgaatcc cgtcgccctt cttcaagcgg cccgcgtagc
     3001 gctcgggcac cgagaagtcg agcttcacgg gtgtgtcgtc ctggatggcc gtgatcgggg
     3061 tggcggggga cacgtagctc ccctcactga cgaagcgcag ccccaccttg ccgtcgaacg
     3121 gtgcccggat ctcggtcttg tcgagttggg cgcgaaccag cgccgcttcc gccttggcga
     3181 cgttgaactc gttcatcgcg gtgtcgaact cgcgctggct cgtgaggctc tgctcgaaca
     3241 gctggcgctg gcgctcggcg tccgactcgg cgatggcgag gcgcgactcg gcgcgcgcgg
     3301 tctgcgctcg cagctccgcg tccgcgatct tcaccagcag ctggcccttg gcgacgcgtg
     3361 acccttcggc gaaggagatc gcttcgacgc ggccggagat ctcgctgcgt acgtcgacgt
     3421 cctcgttggc caggatggtg cccacggtgc tgatgcggtc cgtcatggac tgcgtgccca
     3481 gcaccaccgc gcgcaccgcg gtgacgccgc cgcccgctcc gccgggtccg ccgccggcgc
     3541 ggccgccctg acgcgaatcg ccctggagca ggcccacctt gatcaaaacc agcgcggcga
     3601 cgacgatcgc ggcgacgacg agcgacgtgc gtcctgtagc gaacttcaaa tctgaacctc
     3661 cggctgcggg tcctgtcgct tccgcacccc gctgcgtacg catcggggac cccgcgtcag
     3721 cgccacccgc ggccagtctc gggacccaaa cgacgtcagc cgatttcgag catccggtcc
     3781 agcgcgcgct tggcgcgcac ccggatgtct tcgggaacct cgatgacgta ccgattgtgc
     3841 agcagcgcgt cacgcgtgtc ttccagcgtg atttcgttca tgtgcgggca ccgcacgctg
     3901 cagagccgca ccgtctcctt gtccggattc tccgcaatga tgttgtctcc catgctgcac
     3961 tcggtgagta ggagataacg gggcgcgttc gacttcttga cgaactcgat catcttcgac
     4021 gtgctgcccg agaagtcgga ggcctgcacc acctcggcgc tgcactccgg gtgcgcgagc
     4081 accgccacgt cggggaactg cttgcgcacc gcgacgatgt cggcgacggt gaacttctcg
     4141 tgcacctcgc agcggccgtg ccacccgatc aagtggtaat ggagattgcg gggtgccgcg
     4201 tcgcgcgcgc ccgacgattt gggttcgcgg gtcgggaaga tgacctggcg gccggtttca
     4261 cgcgcgacgt tctgcgcgag gtactcgtcg ggcaggaaga tgacgcagtc ggcgccgagc
     4321 gcttccacca cgcgggccgc gttgctcgac gtgcagcaca cgtcggtctc ggctttgacg
     4381 tcggcgtagc aattgacgta ggtgaccacc ggaaccccgg ggtactggcg cttgagctcg
     4441 cgaacgtctt cggccgtgat gctggccgcg agtgagcagc ccgccttctt ggccggcagc
     4501 agcacggtct tattcggatt gagaatctta gccgtctcgg ccatgaagcg cacgccgcag
     4561 aacacgatgg ggtccttgtc ggtccgtgcc gccttgcgcg agagttcgag ggaatcgccc
     4621 accacgtcgg ggacggtgtg atagagcgcg ggctccatgt agttgtgccc gagaatgatc
     4681 gcgccgcgct cttccttgag ccggttgatc tcgaagacca actccgcctt gatgcgcagc
     4741 tccgcttcgg gtacgacgcg cgcgagcttg tcgaccatga ggcggtaggt ctcttcgatg
     4801 gtggcgccac gaatcataat ggaagcagac tccccctgaa gatacgccga agtcgtcccg
     4861 tttgcaattg ccgcgtggat cgcaacctaa cgcagtaaaa cgacttgggc gcacgtttga
     4921 cacgtttgta tccggcgcgc tatggttatt gtcacttgcc agcagccccc gcggcacctc
     4981 acgtcgcgga ctgaaaaggg ggctatcatg catcgtccat ctcccacgcg cgcactgttc
     5041 gccgcagccg ctatccttat acccgcgacc gtcgactttt gcttcgcaca accgcttgcc
     5101 aaacccaaac cgggcgattc gccccgcggc gtcctcgtgc tcgacggatc gccggtccac
     5161 gacgtcggca tcctgcgcgt tcacgcctcc aattgggggg cgatcggttc ccagccgggt
     5221 tcggcgggtc ctttttcgga agctccatcg gccgaatggc cctcgggttc gcgggtcgag
     5281 tatctctacg tcgcgggaat ctgggtcggg gcgctgctca acgggatacc cgcggtgtcg
     5341 acggccgcgt ttgcgaccga attccggccg tcgtccgatc cgcgcgacat cgtgtacgag
     5401 tcgcatatcg gcgtggccgg cggccatcgc gtcccgtccg ccaacgcgga cgacgatagc
     5461 gacggattcc tcgacgagga tccgctggac gggttcgaca atgacctcga tggccaggtc
     5521 gacgaagact atgcggccat ctcggaccag atgctggcgc gtcgctttcg cgacgacgat
     5581 ccatcggtga tcctccccga ccacaatccc atgggcctgt tcgtgcgtga ggagagctat
     5641 caattcgacg accccggtta cgacgatttc gtcggcttca cgttcacgat caccaacgac
     5701 ggcgcaggga ctctccggga cctctacgtc ggcgtgttga tggacggcga tgttggacac
     5761 cgcgacacgc cgaactactg ggaggacgac gccacgggat tgctaacccg tgttgtctat
     5821 cacgatccgc atccgttccg gatttaccgc ttcccgtact ggtacgacgt cgacggcgac
     5881 ggcggccagg ccgacgccta cgccggcatc gtactgctgg atcacccggt cgatcccacc
     5941 ggcacgacgg cacccaccga cgttgacgtc cacaccgttt cgtacttctc cggcagcctg
     6001 ccctacgaag aaggcggcga tccgaccaac gacttcgagc gttacgaggt actctcaaat
     6061 ggggtcattc ggcagcctcc cgtatagcca agcgactacc gcgcgctttt ttcggtcggg
     6121 ccgttcgacg agattgcacc cgggcagacc atcgaggtct ccttcgcgct ggtggtaacg
     6181 ccgcgcgacg acttcaccca cgtcgaccgc gcagccgtcg cgtatcacgg actctggttc
     6241 gatttggacg atgatcctgc caccggaatc gacggcaaag agcaccagga acattggtat
     6301 ctgcccaacg acaatccgac gccaatcgcg atcacgcgct tcgaggcacg cgcactcgat
     6361 ccgcaccgcg tgaaggtgga gtgggaggcg tcgtcgagcg acggaatcga gtcgttcgat
     6421 ctcctgcgtg cgacctctgg agacgcgctg cgtcgcgtcg tgcccggcac cttcggtccc
     6481 gcgacgagcg tctttgtcga tgagacggtg accccgggcg gacactacga gtacgtcgtg
     6541 gttgcgcgag acgtccgcgg cgcggagttc atctcgcccc ggatcggcgt gacggttccg
     6601 agcgtggtgc tctcactcgg ccagaactcg cccaatccgt tcaacgaatc gacgagcttc
     6661 gggatcacgc tgcccgaacg cgccgacgtg gacgtgtccg tatacgacgt gtcgggacgg
     6721 cgcgtggcca cgatcgcatc gggcgtgcgc gacgcggggg attacgatct ggattggaac
     6781 gggaccgacg acgccggacg gcgtgtggcg gcgggcgtgt acttctatcg cttggaggcg
     6841 ggtgggcgga cgctgacgcg caagctggtg ttgacgcgct aatcgttctt ctcttcgacc
     6901 caacgcccca ccgcgccgtc gcgatgcgag gcggtgagct tcgccgcgga gccttccgtg
     6961 gcacccgcgc cccagcgggc ttcgatctcc ttcatgagct cggagaccat gtcctcttct
     7021 tgcaccttgc gcgcgatttt cccatcgata tagatcaatc cctcgccgtc gccggccgcg
     7081 atgccgatgt cggcgccggc cgcctcgccg gggccgttga ccgcgcaccc catcaccgcg
     7141 atgttgatcg gcttgcgcag gtgctgcgtc ttcttctcga cttcctccgc gatgcggaac
     7201 aggtcgatgc ggttgcgcgc gcacgtgggg cacgcgatga tgcgcggcgt gttcgactga
     7261 atgtgtagcc cctgcagaat cttctgcgcc acgcgaacct cgtcgaccac gtcggcggcc
     7321 agcgagacgc gcatggtgtc gccgatgcct tcgtgcagga ggatccccaa gcccaccgag
     7381 gagatcacgg ccccgctcaa tcccggcccc gattcggtaa tgccgatgtg gcgcgggtac
     7441 tgcgatttcc tcgagaacaa acgataggcc gcgagcgcga gcggcacgtg cgacgccttg
     7501 agcgacacga tgatgtcggt gaaatcgtgc tcctcgagca ccccgatgtg gcggaaggcg
     7561 ctctccacca tggcttccgg ggtggggaag ccgtacttca tgagcaggtc cttctcgagc
     7621 gagcccgcgt tgacgccgat gcggatggga agtttgtatt ccttggcctt cgcgaccacg
     7681 gcggccaccc gttccttgct gccgatgttg cccgggttga tgcgcagctt cgcgatcccg
     7741 gtacccgcca cctcgaccgc cattcggtag ttgaagtgga tgtcggccac cagggggacc
     7801 cggatctgac gcacgatctt ctcggtggcg cgcacactct cgatgtcggg gacgctcacg
     7861 cgcgcgatgt cgacgcccgc gtcttccatg cgcttgatct cgtccaccgt gcgctggacg
     7921 tcggtggtcg gggtggtcgc catcgactgg atggagatgg gggcgccgcc gccgaccgcg
     7981 accgcaccca ccatcacctg tcgggttgcg agataggtgg tttgactcac gatgcttccg
     8041 ttggctggag tggggacctc gcgggcttcg atccgcatcg aatctacacc ccgtgcgcgc
     8101 ccggacgcaa gtccggaacg gtcctagtct caacgatgat ggcgggttac gaggccgcgg
     8161 caggcgtggg tcgaaaaaac gacgaagccc ggaacggatc cccttcgatc ccgcgcccgt
     8221 gaaagaggga gtaaaaaccg agcgcgagtc gaagagaatc ggttccgggc ttcgcccgaa
     8281 gaatccggcg ggtcggtgcg ggactcccga accagtggtc ccaacaccgg gctgctatcg
     8341 cgagatacca ggtcctaacc cgccggaggc tctcgcctag aaattaaatt ccggtggtcg
     8401 atccctacgt ggacttacgc ccccaccgtc cacgttgctg taccgccaga gatgctcgac
     8461 caccggaatc tgatcgtcaa atccggggga atcaaacgac attcgcggta accctacaat
     8521 aaggcattaa tggggcttga ttgggtatta actccctggt ttatccggct agtgcgagcg
     8581 caggtacgcc gccaggttga tctccctatg atcgagctgc agcttacgcc gaatcgactg
     8641 ccggtgcttg tgcacggtct gcgatgaaag tcccaacgcc tccgcgatct gcttcgagga
     8701 gcgcccggtc ttgatcatat ccagaatgtc catctcacgc ggtgtgagct tggatacatt
     8761 gttccggtaa tcgttgatgt gcttgcccgt gatctcgtcc aggccgtgga ccaggacgtc
     8821 gatgtcccgg cgcgacaaac ggccgtcctg ctggagctta cgcatcgtgg ggcgcagcag
     8881 gttgtcgatg ttcgtgcaga tctcgtgtcg gtacttcgcc ttttcttcgt cgatgtggtc
     8941 gacgacttcc tggaacgcga tattcttgcg caggacctcc tgatgctctt gctggagctt
     9001 ctccgtcgtt tgccgcagct tctcgtcggc gatgacccgg tcggtgatat ctcgggtcac
     9061 ggccagaaag cacgggcgtc cgtcgagatg aatgacctcg gccgagacca gacacgagag
     9121 caccgctccc ccgcgcgcga cgaactcgat gaacatgtcg cgcacgcgac cgtcgcggcg
     9181 aacgacgtcc gccaggtgtt tgcgcacctc gaggtccttc cagattccca actcgagggt
     9241 ggtacggccg acgatgtcgc tgcgacggta ccccgtgatc cgctcgaagc cgtcgttcac
     9301 ttcgatgatg cggccggtct cgatatcggt gatggtgatc gagtcggggc tgttccggaa
     9361 cgccttctcg aagcgctcct ccaggagtcg gagctggttc tcggactcat ggcgttcgct
     9421 gatgtcccgg tagttcatga cgatgccgcg cacatcgacg tcgtcgacga ggttctggag
     9481 gacggcctcc atggtgcgcc acgacccgtc cttgtggcgg gcgcggatgg ggccggtggt
     9541 ttcgacgctg ccggactgcc tgacggattc gagggcttgc gtcgcgcgct cgaggtcgtc
     9601 ggggtgaacg aaatcgagcg ggttggtgcc gatcaactcc cgcggctgat aacccagcac
     9661 gcgttcgacc gacggactga cgaaaaccgt cttggcttgc gggtcgacaa ctttgatcac
     9721 atcgaacgcg ttgcggatca atgcttggaa gagaagcgcg acgcgcgcgg catcgagcga
     9781 ctcttgagta tgctggttca taaaactctc ccagcgctct gccgaggggc gatgaagggg
     9841 ttcggtagag aatcggtacg ccctaaggat attccccacc gcgccaggaa tcaagcacct
     9901 cggtcggtgt acgtatgcta tcctcaattg cgagttggag ttatacctta acaatggctc
     9961 gaatagagcg gccgaatgag cgacgcggat tcaactaatc ggctggaaga ggcggcggag
    10021 aagtttccgt ccgaccccgg cgtctatcta ttcaaggacg acgtcgggcg ggttctctac
    10081 gtcggcaagg cgcagtcttt gcgcgtgcgc atccgcagct acttccgcgg cggcgacgaa
    10141 cgcgcgagca ttcgctactt gatgcagcgc gcgcgatcgc tcgagtacat cgtgaccgac
    10201 accgagcaag aggcgtacat cctcgagaac aatctcatca agaagcaccg gccgcggtat
    10261 aacgtcgtct tcaaggacga caagacctac gtcaatttgc gcctcaacgt cgaccatcca
    10321 ttcccgcgcc tcacggtcgt gcgccggccg cgacgcgacg gagccgtgta cttcgggccg
    10381 ttcgcgtccg cgggctcggt gcggcagacg ctgcgcacta tcggccgtat ctttccgttg
    10441 cgcacgtgca ccgacgcgga cttctccagc cgcacccgcc cgtgtctcta tttctacatc
    10501 aagcgctgcc ccgcgccgtg cgtgggcaag atcgacgccc aggagtacgc agagaccgtc
    10561 aagcgcgtga cgatgttcct gaagggacgc ggcgacgagc tcgtcaaatc gctgcgcgac
    10621 accatggaac gccagtccgc cgagcgccgc tacgaagagg ccgcgcgcac gcgcgaccag
    10681 gtattcgcga tccaacgcgt ggtggagcgc cagcgcatca cctcgcccca gcgcgcggag
    10741 cgcgacgtct tcgccgcgta tcggcgcggc gagcgtctgg tcatccagct gttgtcggta
    10801 cgcgagggcg aagtgcagga cggcgagacg ttcgtgttcg ggagtgcctt ggaggcgacg
    10861 ggcgagcact tggcgtcgtt catcaatcag tactaccagg ctcgcgccgt catccccgac
    10921 gaggtcctcg tccccgaaga gatctccgat cgcgcgtcgc tggaggcgct gctgtccgag
    10981 cgcgccaaac ggcgcgtcga gattgtggtg cccaagcgcg gccagggcgc gaccctcctc
    11041 gaattggcgc gcaagaacgc gcgcgttgcc ttcgaggaag gacgcaccga gcgtaaccgg
    11101 gagctgctcg aggatctcca ggtgatcctc ggactggagc actatccgcg ccgcatcgaa
    11161 tgcgtcgaca tctccaacat ccaggggacg aaagcggtgg gctcggcggt ggtgttcatc
    11221 gacggcgagc cggcgaaagc gcactatcgc cgcttccgga ttcggaccgt cgagggttcc
    11281 gacgacaccg gcatgatgcg cgaagtcctg gagcgccgcg tgacgcgggg cctcaaggag
    11341 ggcgacatgc ccgacctcct gatcgtagac ggcgggcgcg ggcagctcaa cgtggccctc
    11401 gacgtgctga agcggctcgg cgccgacacc gtcgcggcca tcgggatcgc caaggtgcga
    11461 aacgagaaga accgcaagat tcgcggccag gagcgcgttt acctgccggc catcgaggag
    11521 ccgttgctat tggaccgcca atcgcaggcg ttgtatctcc tggagcgcgt gcgcgacgag
    11581 gcgcatcgct tcgcgatcgg ctatcaccgg accttgcgcg cgaaggcgat gggggaatcg
    11641 aagctggacg ggattccggg ggtgggtccg accctcaaac agaggttgct cgcccgcttc
    11701 ggaagcgtcg agcgcatccg caaggcgcgt ctcgacgagc tggccgccgt ccccggggta
    11761 tcggtgcgca aggcgcaggc ggtcaaggac gccctgggat gagtgctatc cgctgtgctg
    11821 cccggccctt gggcgggtgg aaggcgttga cttttgaccg gccccggcct atgatgcctg
    11881 tgattctcgc cgcacgtcgg cggctccccc cacagtctcc aaagcgcacg caggccatcc
    11941 tttcggaggt tcgcaagttg gaatcgaaac gaacgctcgc cgttcttctt tcgcgacaca
    12001 tggcccaatt cgccgccctg tcgctggtcg ccgtactcgc ggcctgcgac gaagagacca
    12061 cggtcgagcc ccccgcgccc ccgccgcaga tccagaccac gcagtcgtac tccaagagct
    12121 cgaccgggct gcccaacgtc gaggtcttcg cgatgctcac gctgagcaac ggcgagttct
    12181 ggatcggcac gtcgcaagga atcgcgcgct atccgtcggt caccgcgacc caacgcattc
    12241 ccggcgccga cggtgtcatc aacgagctga acggtctgcc caatccgaag gtccgggata
    12301 tcgtcgagat ggatggagtg gtctacgtgg cgacgtgggg tggcggcatc gcgatctacg
    12361 atatcaccgc gggaacgtgg gaatcgcgca cccccgccga cgatactgcc gacgacgtgc
    12421 acccgcagaa tgtcagtctc gggcgcttcg tggcaccggg gacggggatc atcgtgcagc
    12481 cgggccccaa cgggattctc gagaccgtgc cgcaagggga cgaccgcatt tcgggtaaca
    12541 atatcatcga cggtgcgggc ggcaacggga tcgcggagac gacggctcat cgcgggctga
    12601 acggcgcgat cgccgatctc gagccctcgc cgatcgaggg caagctctac cttgccacca
    12661 acgatgcgat cagcatctac gacccggctc tggaccggtt ctccagcttc acgtcgctga
    12721 cgcgagaggt ggtctcggcg gtgggcgttc gtgacacccc ggggggcatc gaacgctggt
    12781 acggcccccg ggtcgagacc gtcgaagacg atcctccgcc accgatgccg gccgcgggca
    12841 tcaccgtctg gcgcggcgtg tcgacgttct acacgtatac gaaggtcaac tccggcctcc
    12901 cggagccgaa cgtcaacgcg atctatttcg acgccgacgc gggcccggac atcctgtggg
    12961 tggctaccgc gaatagcggc gtctcgcgcg tgcaagtcgg cgcgagcacg tggacgacat
    13021 acaccgcgct acaggggctc ccgtcgaata cggtctactc ggtggttcga gccgctgcgc
    13081 cgggaggggg aagcacgatc tgggtcgcga cccagaacgg cgtggcccgg ctcaggggcg
    13141 acggaacctg gcagggatac aataccagcg gcggcctggc ggccgaccgc gtgcgccggg
    13201 tgtactccga cgacgggctg cggctttgga tcgggtacat cgacgcggga gccgcaaggc
    13261 tcaacgcgtc gggcgccaag taggaccact cggtgcgtct cacgggatct cgaagaactt
    13321 cgggaaccgc gttaggttgg tgatgccgcg ccgagtcacg agcacggtgt cttcgatgcg
    13381 cacgccgccg tgcggccagt agtacaaccc gggttcgacc gtcaccacca ttcccgcttc
    13441 cagtaccgcg tcgttggccg cgacccgcgg tggctcgtgg atctcgagcc ccagtccgtg
    13501 gccggtccca tggaagaatc cctgcatgcg gccgtcgatc ttgccgcttt ggaacccgcg
    13561 cgcggcgaag agcgcgtgaa tggcgtcgtg gatgtcgcgc acgcgcgcgc catggcgaat
    13621 cgaccgcagc gcgaggcgct gtccgtcgtg gacggcgtcg tacatggcgc gcaccgtctt
    13681 gctcgcgcgt cccttgacga aagtgcgggt gatatcggcg aagtagccgt tcttttgtgc
    13741 gcgcggaaag atgtcgacga tgatgggaac gccggcgcgg atcgggccac tgccttcgtc
    13801 gtgcgggtcg cagccctggc gtcccggcgc cacgatggtg tgggcgggta ggcagccgtg
    13861 cgcgagtatc gtggtgtcgg cggcccggcg cagatcttcg gcggtcagtc gccgcccatc
    13921 cagcacgacc cagcccttgc gaacgcgggc gcgccgcaac gtgtcgaccg ccgcctgcat
    13981 gccggcctcg gtggcgcgca tcgcgtcgcg aatcgctttc acctcggccg cgctcttccg
    14041 ggcgcgctcg ggaaacaagg ggccgtcggc gacgtagacg gcgattcgcc gtttgcgcag
    14101 gcgctccgtc actccggacg gaaagctcgc gggaaccgtc accgacccga tccgcagggc
    14161 gcggagaacc tccgcgatgg tgtccgcggc gtgcggggtg cgcttccacc gcttctccgc
    14221 cagcgccgcg tagcgcgcaa aggacagcac gcggtgcgcg ttcgactgcc gcttggcgcg
    14281 gtcgatctcg agatcgctca taacgaggat gcgccggccg gcgccggtgc gcaggaagac
    14341 gaaggggtcg ggggcaaaga atcgcgttgc gtagagcgag tcggcgtcgt tatcgctggc
    14401 tgcgtagagt aaaatggtag acggtcgggc ggacgctcgg gccacggagg aacctccttc
    14461 gaaggggcca tcatcgcacc cgccgcgccg cgctgtaaag agacgggaat gaacgacaga
    14521 cgcatcgatc tgcacatgca ctcgaacttc tcggacggcg tccacgcgcc ctcggtgctg
    14581 gtcgacctcg cgaagtggaa gggcatctcc gcgatcgcgc tgtgcgacca cgactcgctg
    14641 gacgggtatc ccgagctgga ggcgtcggcg cgcgccgccg gggtcgaagt gatcaccggg
    14701 gttgaactct cctgcgagca tgcgggccgc gatttgcacg tgctgggcta cggggtggac
    14761 cccaagagca gcgcgctgca ggattcgctg cgctcgttcc gcgcgtcgcg cgagcagcgc
    14821 ggcgtgcgca tcgtcgcgct cttgcgcgag cagggcgtcc acatcgagat ggacagcgtg
    14881 ctcgcgaaag cgggcggcgg cgcactgggg cgcccgcaca tcgccgaagc attgctcgaa
    14941 gccggctatg tgaacgacca cgccgaagcc ttcgcgaagt acatcgggga gggtggaccc
    15001 gcctacgtcg agaagtacaa gatgagtccg gccgaggcgg tggggagcat ccacggggcg
    15061 ggtgggctcg cgttcgtcgc ccaccccggc tactacctgg acaacgggga ttccttcggc
    15121 gtgctgctcg accacggctt cgacggcgtc gaagtgtttc acccctacca tcgcgcgtcg
    15181 gtcatcgagc gcctgctcgg gatcgcgcgc gagcgcgggc tcttgatttc cggcggctcg
    15241 gacttccacg gcttcgccgg tcgtgataat ctgggcgagc ccccggtgtc gtacgcgctc
    15301 ttggagcgca ttcgcgacac cctcgaggcg aggccacgat gatcgaagaa ggcaagaccg
    15361 cgcccgattt cgccctcacg gccgacgacg gcacgaaggt gacgctgtcc gcgttgcgcg
    15421 gaaagcccgt cgtgctgttc ttctacccca aggccgacac ccccggctga acggccgaag
    15481 cctgcgattt ccgtgacgaa atcgccgcat tccggagcaa gaaggccgtc gtcctgggcg
    15541 tcagcaagga ctccgtcaag gcccaggcca agttccgcgc caagtacgag ctgccgtttc
    15601 cgctcttgag cgaccccgat gccgcggtgt gcaacctcta cggcgtgatc caagacaaat
    15661 cgatgtacgg gaaggtggtg aagggaatcg agcgctcgac cttcgtgatc gacgccgggg
    15721 gtcgcgtggc caagatctat cgcaaggtca aggtcgccgg acacgcgcgc gccgtgctgg
    15781 acgatttgta gaggcgagca cgcggtaacg taagaacact tcttctcccg cgcgcttgac
    15841 ggacacaaga cgcaggcgca tgtggtcctt ccacaagaat ccctcgccgt ccaagagcga
    15901 gggcgcttct tttccgccga ttagacgagg cgttacggtc acgtacactt ctcccaccag
    15961 ccccgccttc gcgaaggcga aatgaacctc cccgccgcct tcgaggagga cgcggcgata
    16021 gccgcgtcgc accagcgtcg ataagacgcg ccgcggggac aggtcttttc ccggaagcac
    16081 gatcacttcg gcgacgcgct cgaaggcgcg cacgcgccgc ttgggcgcgt tcgtggtggt
    16141 gaacacgatg cgccgcgtgt ccttcgacgc aaagaaacgc gagcggaggg gaaggtcgag
    16201 agcgcggctc aagacgacgt tgactggatg cggaccgcgc cgcgccgcgc ggcgtgcacg
    16261 caggtcggcg tagcgcaaga cgatggggtg gccgtcgtgg cggaccgttc ccgcgccgac
    16321 gatcacggcg tccgcgcgcg tgcgcaactc gtccatcaag cggcgatcgc gctcactgcc
    16381 gagtgcgacg tgttcacgcg cgcgcgtggt gatgcgccca tcgaggctca ccgccatgtt
    16441 gacgacgact gtgggcagcg gcgacgagcg agcggacttc gccaagggtc cgggaccttc
    16501 tagcgaaaca cgcgccggaa gcgctcgacc gcgtcgtgga tcacgggcac cggtgtggcc
    16561 gagaacgaca gccgcacgtt gtcgccgaag tgcttaccga agcacgagcc gtatacgctc
    16621 gcgatgccgt gctccatcag gagctcgtag acgtagtggt tccgctccgg gcccgagagc
    16681 tcgcgcggaa tgaactcgga gaagtcgggg aacatgtaga aggcccccgc gggccggtcg
    16741 atggcgacgc cgggcaggtc cttcaatccg tcgtggagcg cgtcgcggcg gcgctggaac
    16801 tccgccacca tggtcgcgac cgcgcggcgg ctctcgtcca cgttgttcat cgcctccgcg
    16861 gcagcgtact ggagaaacgt ggtcacgccc gcggtctggg tgtagttgcc gagccgcagg
    16921 agcttcgcga ccttcgcgtt gcgcgtcacg acgtagccc
//
LOCUS       DASPGK010000427        11687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1157801, whole genome shotgun sequence.
ACCESSION   DASPGK010000427 DASPGK010000000
VERSION     DASPGK010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157801"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..2308
                     /locus_tag="VEC56_11860"
     CDS             308..2308
                     /locus_tag="VEC56_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXV14890.1"
                     /translation="MQSVDTKSLPENAYKPLKPGEAYHPVIPAGTQVPEVTFRSVFWG
                     TFLCVIFTIASVYSGLKVGQVMEAAIPISILAIGLARMYPRRSSILENVIITGIGGVS
                     GSVVAGAIFTLPALYILKLDPHPLQSTFICLAGGCLGVLFLIPLRRYFVRDTHGQFPY
                     PEATAITEVLVTGEKGGSQAKLLLQATFLSGIYDFFVTTYQVWAEVINFRFIPVVDKI
                     ANRAKVVFQFDAVAFILGLGYVMGLRSSMILCAGGVLSNFVLVPMIWLVGRNFPDTLV
                     APGTTPIVDMTADQIFRSYVRFIGVGAIATAGIFAIVKSLRVVAGSFGVAMKAFRGGE
                     GAAVERTDRDMSVMSILVGVVLSAIGVAVLFATLGTSPMVVVIGLVLAVFFSFFFTSV
                     AALCIATTARNPVSGMTMLTIIVSSVVLLQFGLSGTTGMFFVMMIAGMVCTALSVSGQ
                     TITDLKTGYWLGSTPMRQERVKFIGVIASAVAAALTIAVLARVYAFGETEPGDTRKLL
                     DAPQAAIMKALVEGFMSRDPIPYILFGAGAMVTIMMEMLGVPALIFALGMYLPLYLNS
                     PALAGGILAHYLDKRANRVGGNRGRSIRERGIVIASGLMAGGALGGVIGAAMRLIPGY
                     ADTDIKSPFYDNAAISQIVSTVGFLGFVWYTWWASQRVPKDS"
     gene            complement(2313..3341)
                     /locus_tag="VEC56_11865"
     CDS             complement(2313..3341)
                     /locus_tag="VEC56_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14891.1"
                     /translation="MSLLLVEDLFLRFRGQARPAVDGLSFAVERGEIFGLLGPSGSGK
                     TSTLRMIAGFERPERGRIVLDGRAIEGDGAFVPPERRNVGFVFQEFALFPHLTVMENV
                     MFGLHGLPRGERAGRAMEILERAQLAEHAARYPRQLSGGQQQRVALARAMAPRPHVIL
                     MDEPFGSLDPDLREDARRKIADVFREHAMSAVFVTHDQTEALGFADRIGVMREGRIEQ
                     VGTPEELYNKPRTRFVASFIGGSNILSGIGRGDCVMTPIGRVPIDRDATGNVTIALRP
                     EHLQLEAATAGEPSGQVVSRVFHGHDLTIRVAFAEAEVSVWDDYRCPFRVGEAVRVRP
                     REKGVVVE"
     gene            complement(3341..4897)
                     /locus_tag="VEC56_11870"
     CDS             complement(3341..4897)
                     /locus_tag="VEC56_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXV14892.1"
                     /translation="MKRTPPWFLTVPALAVGLAVLVPIGYLAARAFDADPATLRDLVW
                     RARNLELLANTLALASVVIAASTLVALPLAWLTTRTDLPGRFVVTLLSILPLAIPGYV
                     VGYALLGIGAPHGPLSSIFGVGGPRLSGFVGAAVALTIYNFPLVYLHLRTAFASMDPS
                     LTDAARSLGLSPRRVFERVTLPQLVPAYLSSVLLVLLYVLGDFGVVSLMRFETFSYAL
                     YLQYIASLDRTYAAWLALMLLAITTTLVVAEFRLLRDLALERTGLGAPPKARPRRLGA
                     WTTPAYAFIGVVALASVALPVWSIVYWMGQRGAWTDANELVRSFAGSLSVSAPAAFLA
                     TLLAVPMAFLSRRHASGLTHVLERSAYIGYATPALAFALGWVVVVLRAAPWFYQTAAL
                     LVLAYTLHFLAQAIGPVRAGLHVATPRIEEASRSLGLGPLATFRRVTLPILRPGLTAA
                     AILVFLSCLKELPLTIILSPLDFETLALNMYAFTTEAMFAEAAPYALSIVLLSALLTS
                     ILFHKVGEER"
     gene            complement(4912..5958)
                     /locus_tag="VEC56_11875"
     CDS             complement(4912..5958)
                     /locus_tag="VEC56_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HXV14893.1"
                     /translation="MRTRIGRSTIAAMCIAVLAGGCGGEKKTAEESAPATAASTTLVL
                     YSGRSEGLVEPVIALFEKETGIDVQVKYGNTPELALTLREEGERGGADLFWAQDAGAL
                     GAVSKAGLFADLPADVVAGVPELFKHSGGKWVATSGRARVLAYSPERVKPEQLPKSVF
                     DLTDARFRGRVAWAPTNASFQAFVTAMRKTHGEDTTRTWLEAMKKNGTHAHANNVAIL
                     QAIARGEVDYALPNHYYLLNFKKSEAKFPVDQVAFAPGDVGNLINVAGIGILATATHR
                     DAAERFVRFVLSPTAQQYFASEVSEYPVIDNVMTNARLVTREELLKSAPRVTLDDLDD
                     LDGTLALLRSVGLL"
     gene            complement(5955..6653)
                     /locus_tag="VEC56_11880"
     CDS             complement(5955..6653)
                     /locus_tag="VEC56_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV14894.1"
                     /translation="METRPLIRFLGVSTALHAVAGALAIAFVSIGEGDGAAVQPTVEV
                     TLVAMADEVAATVSLTAATSSHASEAVAEVPAATPFAPIDAPTTSARSEAVDDHGTAD
                     TVASPQPRIADSGTGGAGSPPAIVLGTDPAAAGVAGAGGGGDDTSVWAWLERHKRYPR
                     AALQRRLEGEVTLDLVLDGSGRVHEARIVRSSGHRSLDDEVTRMVARADPFPIAGAES
                     REYRIVIEFHLEDP"
     gene            complement(6659..8938)
                     /locus_tag="VEC56_11885"
     CDS             complement(6659..8938)
                     /locus_tag="VEC56_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV14895.1"
                     /translation="MVAMIPQRALARVLVAFLLVVVPSFAVATEEKSDAPPSETSSKE
                     KRADTPETKLLNRITVVGAPGRAAGIPGSVHRISATELAKENQAYDDIHRVMLRVPGV
                     VITEEEGYGLRPNIGMRAAGTDRSANITLMEDGVLAAPAPYAAPAAYYFPITGRMEGV
                     EVRKGSSQIKYGPRTNGGALNLLSTSIPEQTAANARVSAGSNDTRKLYANYGDTWKRV
                     SWLLEAYQARTDGFKNLDTGGNTGFDVEDYVGKVRVASSTEAKHYQEVTLKLGYKDET
                     SNETYLGLTDDDFARDPYRRYAASQFDQMKNDHTQGLVRHFIVASENVDVTTSLYRND
                     FDRNWYKLDRVDGVSLDDVLADPDAHASQYAHLTGDSASADGALAVKANNRTYYAQGV
                     ESIVGVSFDGFGTRNEMEIGARYHEDEEDRLQHTDGYRMQAGGTMVRTSEGVPGAAGG
                     GDNRVGFARAVAGFVQDEITMGAWTVIPGARVEWIETRQDQYAAGDPDRALAPTVTEN
                     STTAVVPGIGATYRATQWLDAFAGVHKGFAPPGPGADDATLPEESINYEAGVRGRTGG
                     VELSLLGYFNDYENMLGKDTFSSGGTGEGDLYNAGAVRAYGLEASVSRTFDAGPFRTP
                     LSATYTYSHAEFLSSFQSAYEPWGTVESGDELPYLPAHVASVSVGVENERALATLFAN
                     YTSEMRTVAGQGDIPSVESTDARVVLDVSAEYGLVSHARLFASMQNLTDEAYIVSRRP
                     AGVRPGLPRTFIAGLKFDL"
     gene            complement(9123..9620)
                     /locus_tag="VEC56_11890"
     CDS             complement(9123..9620)
                     /locus_tag="VEC56_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXV14896.1"
                     /translation="MNKPLTPNMEMYLKTIFEIVGEGTEPRVKAIADRLGVKMPSVTG
                     ALETLQQRGLVAHDRYGDVQLTIRGRKVAREVKDRNDLIYRFLLEVLRLPAATASRDA
                     CVLEHVVSPKTLERLESFLEFLHVCNRGANETIAHFGNWLDCAEKGESCSACSESGGG
                     PRCTS"
     gene            complement(9786..10256)
                     /locus_tag="VEC56_11895"
     CDS             complement(9786..10256)
                     /locus_tag="VEC56_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXV14897.1"
                     /translation="MLNFNVIVVTTDLSEYSLRALPYAVGLAERFKAKLKVVCVNEPA
                     LQVSDVAFVGIDPLISQREHSAAIRDALAKVVAGLPKTVDIEGKVLEGVAVDQVVKYA
                     SEINADLIVSCTHGRTGLSHVLMGSTAEALVRRSPCPVLTLKQPMPVAAAQRGA"
     gene            complement(10272..10730)
                     /locus_tag="VEC56_11900"
     CDS             complement(10272..10730)
                     /locus_tag="VEC56_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXV14898.1"
                     /translation="MIHSPTCQHALRALIYLAEKDAPGPVLVREIAEAADVPHPSLAK
                     ILHSLRGRGLVRSTKGPGGGYQLARPGDQMRVIEVIEAVDGRVDLDTVCVLGLDTCSD
                     EAPCALHDVWKLFRNNYAATLTKMTLREAANVLKAKRSARGVPEARVAQQ"
     gene            10962..>11687
                     /locus_tag="VEC56_11905"
     CDS             10962..>11687
                     /locus_tag="VEC56_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HXV14899.1"
                     /translation="MPIATPFHPRTSAHCVSLFYKDWAGYYAVRSYDTTHDREYFAFR
                     HACGLIDVTPLYKYDVRGDDAARFLARVMVRDIRKLKVNQVYYVCWCDDHGRMIDDGT
                     VSRLSETDYRVTSSEPAWHWLQRTARGYRVSIEDVSDAIAALSLQGPTSRDVLDAVTD
                     GAVAKLGFFRLTRGKIAKADVLVSRTGYTGDLGYEIWMANADALRVYDALFSAGHAYG
                     LEPAGLDAMDVTRVEAGFILNGVD"
BASE COUNT         1890 a         3885 c         3916 g         1996 t
ORIGIN      
        1 acgtccccaa cccattcaac ccaacaaccc gagttcgttt cgcaaccccg accgagggtc
       61 cggtacgaat cgcgatctac gacgcgcgtg ggaccctggt gagagttctc gtcgacaaat
      121 ggttcccggc agggcatcat gacgcggtct gggacggtcg ggacacgaag ggatcaccgg
      181 tgagttccgg cgtgtatctg tcccggctcg agcaccggtc ggattaccga acgcgtaaga
      241 ttgtgctgat caagtagtcg cgcgaacccg cgcgacgctc ccagaaaaga atacagaagg
      301 agggcgcatg cagtccgtcg acaccaagtc gctgccggag aacgcgtaca aacctctcaa
      361 gcccggggaa gcttatcacc ccgtcatccc cgcgggcacc caggtccccg aagtcacctt
      421 ccgcagcgtt ttctggggca ccttcctgtg cgttatcttc acgatcgcgt ccgtgtactc
      481 cggcttgaaa gttggccagg tcatggaggc ggcgattccg atttcgatct tggcgatcgg
      541 tctcgcgcgc atgtacccgc gtcgatcctc cattctggag aacgtcatca tcaccggcat
      601 cggcggtgtg tcgggctcgg tggtggcggg cgcgatcttc acgctgcccg cgctctacat
      661 cttgaagctc gatccgcacc cgctccagtc gacgttcatt tgcctcgcgg gcggctgctt
      721 gggcgtgctg ttcctgattc cgctgcggcg ctacttcgtg cgcgacacgc acgggcagtt
      781 cccgtacccg gaagcgacgg cgatcacgga agtcctggtc acgggcgaga agggcgggtc
      841 gcaagcaaag ctgctcctgc aggcgacgtt cctctccggc atctacgact tctttgtcac
      901 gacctaccag gtctgggccg aagtgatcaa ctttcgcttc atcccggtgg tcgacaagat
      961 tgcaaaccgc gccaaggtgg tattccagtt cgacgcggtc gcgttcattt tgggtctggg
     1021 ctacgtgatg ggcctgcgct cgtcgatgat cctgtgcgcg ggtggcgtgc tgtcgaactt
     1081 cgtgctggta ccgatgatct ggctcgtggg ccggaacttc cccgacacgc tggtcgcgcc
     1141 gggcacgacg cccatcgtcg acatgacggc ggaccagatc tttcgctcct acgtccgctt
     1201 catcggtgtg ggcgcgatcg cgaccgccgg catcttcgcg atcgtgaagt cgctgcgcgt
     1261 ggtggcggga tcgtttgggg tggcgatgaa ggccttccgc ggcggcgaag gcgccgccgt
     1321 cgagcgtacc gaccgcgaca tgtcggtgat gtcgatcctg gtcggcgtcg tgttgagcgc
     1381 gatcggcgtc gcggttttgt tcgcgaccct gggcacgtcg ccgatggtgg tcgtgatcgg
     1441 gctggtgctg gcggtcttct tctccttctt cttcacctcg gtggccgctt tgtgcattgc
     1501 gaccaccgcg cgcaatccgg tctcgggcat gaccatgctc accatcatcg tatcgtcggt
     1561 ggtgctcctg cagttcggtc tctcgggcac caccggcatg ttcttcgtga tgatgatcgc
     1621 ggggatggtg tgcacggcgc tctcggtctc gggacagacg atcacggacc tcaagacggg
     1681 ttactggctg ggttcgacgc cgatgcgcca ggagcgcgtg aagttcatcg gcgtgatcgc
     1741 gtccgcggtg gcggcggcac tcacgatcgc cgtcctcgcg cgcgtgtatg ccttcggcga
     1801 gacggaaccg ggcgacaccc gcaagctcct cgatgcaccg caggcggcga tcatgaaggc
     1861 gctggtcgaa ggattcatga gccgcgaccc gattccgtac attcttttcg gcgcgggcgc
     1921 gatggtgacg atcatgatgg agatgctggg cgtgccggcg ctgatctttg cgctcggcat
     1981 gtatctcccg ctctacctca actcgcccgc gctggcgggc ggtattctcg cgcattacct
     2041 cgacaagcgg gccaatcgcg tgggcggcaa tcgcggccgc tcgattcgcg aacgcggcat
     2101 cgtgatcgcg tcgggcttga tggcgggcgg tgcgctgggc ggcgtcatcg gcgcggccat
     2161 gcggttgatc ccgggctacg cggacaccga catcaagtcg ccgttctacg acaacgcggc
     2221 catctcgcag atcgtgtcga cggtgggctt cctgggcttc gtgtggtaca cgtggtgggc
     2281 gtcgcagcgc gtgccgaagg attcgtaggc gttcactcga cgacgacacc tttttcgcgg
     2341 gggcgcacgc gcacggcctc gcccacgcga aacgggcagc ggtagtcgtc ccacaccgag
     2401 acctcggcct cggcgaacgc gacccgaatc gtgaggtcgt ggccgtggaa gacgcggctc
     2461 acgacctgac ccgacggttc gccggcggtc gcggcttcga gctgcaagtg ctcggggcgg
     2521 agcgcgatgg tgacgttgcc agtcgcgtcg cggtcgatgg gcacccggcc gatcggggtc
     2581 atcacgcaat cgccgcgccc gatcccggag agaatgttgg acccgccaat gaacgacgcc
     2641 acaaagcgcg tgcgcggctt gttgtacagc tcctcgggcg ttcctacctg ctcgatacgg
     2701 ccctcgcgca tgacgccgat gcgatcggcg aagccgagcg cctcggtctg gtcgtgggtg
     2761 acgaacaccg ccgacatcgc gtgctcgcga aacacgtcgg caatcttgcg gcgcgcgtcc
     2821 tcgcgcaggt cggggtcgag actcccgaag ggctcgtcca tcaggatcac gtgcgggcgc
     2881 ggcgccatgg cgcgcgccag cgccacgcgc tgctgctggc cgccggagag ctggcgcggg
     2941 tagcgcgcgg cgtgctcggc cagctgcgcg cgctcgagaa tctccatcgc gcgcccggcg
     3001 cgctccccgc gcggcagccc gtgcaaaccg aacatcacgt tctccatcac ggtgaggtgc
     3061 gggaacagcg cgaactcctg gaacacgaag cctacgttgc gacgctcggg cgggacgaac
     3121 gcgccgtcgc cttcgatcgc gcgaccgtcg agcacgatgc gaccgcgctc ggggcgctcg
     3181 aagccggcga tcatgcgcag ggtcgaggtc ttgcccgacc ccgacggtcc caacagcccg
     3241 aagatctcgc cgcgctcgac tgcgaacgac agcccatcga cggcgggacg cgcttgcccg
     3301 cgaaagcgca ggaacaagtc ttccacgagg agcaacgaca ttagcgttcc tcacccacct
     3361 tatggaacaa gatgctggtc aagagcgccg acaagagcac gatcgagagc gcgtacgggg
     3421 ccgcctccgc gaacattgcc tcggtggtaa atgcgtacat gttgagcgcg agcgtctcga
     3481 aatcgagcgg cgacaggatg atcgtgagcg gcagctcctt gagacacgag aggaacacga
     3541 ggattgcggc cgccgtcaag cccgggcgaa ggatgggcag cgtcacgcgg cgaaacgtcg
     3601 ccagcgggcc caagcccagc gagcgcgagg cttcttcgat gcgcggggtg gcgacgtgca
     3661 gacccgcgcg caccggtccg atcgcttgcg cgagaaagtg caacgtgtag gcgagcacca
     3721 agagcgccgc cgtctggtag aaccacggag ccgcgcgcag caccacaacc acccacccga
     3781 gcgcgaacgc gagcgcgggt gtggcgtaac cgatgtaggc cgatcgttcg agcacgtgtg
     3841 tgagccccga ggcgtggcgc cgcgagagaa atgccatggg taccgcgagc aacgtcgcca
     3901 agaaagcggc cggcgcgctc acggagagcg atccggcgaa ggagcgcacg agctcgttcg
     3961 catccgtcca cgcgccgcgc tgtcccatcc agtatacgat cgaccacacg ggcagtgcca
     4021 cgctcgcgag tgccaccacg ccgatgaacg cgtacgccgg cgtggtccac gcgccgagtc
     4081 ggcgtggccg ggctttcggc ggcgcgccca acccggttcg ttccagcgcc aagtcgcgca
     4141 gcaatcgaaa ctccgccacc accagcgtgg tcgtgatcgc gagcagcatc aacgccagcc
     4201 aggccgcgta cgtgcggtcc agcgacgcga tgtactggag atacagcgcg tagctgaagg
     4261 tctcgaagcg catcagactc acgacgccga agtccccgag cacgtacaac aagacgagca
     4321 gcaccgacga gagatacgcc ggcaccagct ggggcagcgt cacccgctcg aacacgcgcc
     4381 gcggcgaaag ccccagcgaa cgcgcggcgt cggtgagcga cgggtccatg ctcgcgaagg
     4441 cggtgcgcag gtgcaagtag accaacggaa agttgtagat ggtcagggcg acggcggcac
     4501 ccacgaaccc cgagagacgg ggaccgccga cgccgaagat cgacgagagc gggccgtgcg
     4561 gcgcgccgat gcccaagagc gcgtagccca cgacgtaccc gggaatcgcc agcggcaaaa
     4621 tagagagcag ggtgacgacg aagcgccccg gtaaatcggt gcgcgtcgtg agccacgcga
     4681 gcggcagcgc gaccagcgtg gaggccgcga tgaccaccga cgcgagcgcc agcgtgttgg
     4741 ccagcagctc gaggttgcgc gcgcgccaca ccagatcgcg cagcgtggcg ggatcggcgt
     4801 cgaaggcgcg cgccgccaga tagccgatcg gcaccagcac ggccagcccc accgcgagcg
     4861 cgggaactgt gaggaaccag ggtggcgtgc gcttcatccg tgctccgctc tttacagcaa
     4921 tcccaccgac cgtaacagcg cgagcgttcc gtccaggtcg tcgaggtcgt cgagcgtcac
     4981 gcgaggcgcc gatttcagca attcctcgcg tgtgaccagg cgcgcgttcg tcatcacgtt
     5041 atcgatcacg ggatactcgg agacttcgct cgcgaagtac tgctgcgcgg tcggcgacag
     5101 cacgaagcgc acgaagcgtt cggccgcgtc gcggtgggtg gccgtggcca ggatgccgat
     5161 gcccgccacg ttgatcaggt tgcccacgtc gccgggcgcg aatgcgacct ggtcgaccgg
     5221 gaacttcgcc tcgctctttt tgaaattgag caggtagtag tggttgggca gcgcgtagtc
     5281 cacctcgccg cgcgcgatcg cttgcaggat ggcgacgttg ttggcgtgtg cgtgcgtgcc
     5341 gttcttcttc atcgcttcga gccacgtgcg cgtggtgtcc tcgccgtggg tcttgcgcat
     5401 cgcggtcacg aaggcctgaa acgacgcgtt ggtcggcgcc cacgccacgc gaccgcggaa
     5461 gcgcgcgtcg gtcagatcga acacgctctt ggggagctgc tcgggtttca cgcgttccgg
     5521 cgagtacgcg agtacgcgcg cgcggccgct ggtcgcgacc cacttgccgc ccgaatgctt
     5581 gaagagctcg ggcacacccg cgacgacgtc ggcgggcagg tcggcgaaga ggcccgcctt
     5641 cgacaccgcg cccagggcac ccgcgtcctg cgcccaaaac aagtcggcac cgccgcgctc
     5701 gccttcctcg cgcagcgtca gcgcgagctc gggcgtgttg ccgtacttga cctgtacgtc
     5761 gatgccggtc tccttttcga acagcgcgat caccggctcc accaggcctt cgctgcggcc
     5821 cgagtagagc accaaggtgg tggacgccgc cgtcgcaggc gccgattctt ccgccgtctt
     5881 cttttcgccg ccgcaaccgc cggccagcac tgcgatgcac atggccgcaa tggtcgaccg
     5941 tccaattctc gttctcatgg gtcctccaaa tggaattcga tcacgattcg atactctcga
     6001 gattccgcgc ccgcaatcgg aaacggatcg gcgcgcgcga ccatgcgcgt cacttcgtcg
     6061 tcgagcgagc ggtgcccgct cgagcgcacg atgcgcgctt cgtgcacgcg gccgctcccg
     6121 tcgagcacga gatccagcgt gacttcgcct tccaggcggc gctgcagggc cgcgcgcggg
     6181 tagcgcttgt gcctctccag ccacgcccac acgctcgtgt cgtcgccccc gccgcccgcg
     6241 ccggcgacgc cggcagcagc cgggtccgtc cccaagacga tcgccggcgg cgatcccgct
     6301 ccgcccgtgc cggaatcggc aatgcgcggc tgcggtgacg cgacggtatc ggccgtcccg
     6361 tggtcgtcga cggcctcgct tcgcgcggag gtggtgggcg cgtcgatagg cgcgaagggt
     6421 gtcgcggccg gtacctccgc cacggcttcg ctcgcgtgcg acgacgtcgc cgccgtcaac
     6481 gagacggtgg ccgcgacttc gtccgccatg gcaaccagcg tcacttcgac cgtcggctgc
     6541 acggccgcgc cgtcgccttc accgatactc acgaacgcga tcgccagcgc ccccgcgacc
     6601 gcgtggagcg cggtcgacac gccgaggaat cgaatcagag gcctagtttc catgtcgtct
     6661 ataaatcgaa cttcagtccc gcgatgaacg tcctcggcaa accggggcga acccccgccg
     6721 gccggcgcga cacgatatac gcttcgtcgg tcaggttctg catgctcgcg aacaggcgcg
     6781 cgtgcgaaac gagcccgtac tcggcgctga catcgaggac gacacgcgcg tcggtgctct
     6841 cgacgctcgg aatgtcgccc tggcccgcga ccgtgcgcat ctcgctggtg tagttggcga
     6901 acaacgtcgc gagcgcgcgc tcgttctcga cgccgacgga gacgctggcc acgtgcgcgg
     6961 ggaggtaggg cagctcgtcg ccgctctcga ccgttcccca cggctcgtat gcgctctgga
     7021 acgacgacaa gaactccgcg tgcgagtagg tgtacgtcgc cgagagcggc gtgcggaacg
     7081 gacccgcgtc gaaggtgcgg ctcacggacg cttccaggcc ataggcgcgc accgcgcccg
     7141 cgttgtacag gtcgccttcg cccgtcccgc ccgacgagaa ggtgtccttg cccagcatgt
     7201 tctcgtagtc gttgaagtag ccgagcagcg acagctcgac gccgcccgtg cgcccgcgta
     7261 cgccggcttc gtagttgatg ctctcctcgg gaagcgtcgc gtcgtcggca cccgggcccg
     7321 gaggcgcaaa gcccttgtgc acgcccgcga acgcatccag ccactgcgtg gcccgatagg
     7381 tggcgccgat gccgggcacg accgccgtgg tcgagttctc ggtcacggtc ggcgcgagcg
     7441 cgcggtccgg atcgccggcg gcatattgat cctgacgcgt ctcgatccac tcgacgcgcg
     7501 cgcccggaat gaccgtccac gcccccatgg tgatctcgtc ctgcacgaac cccgctaccg
     7561 cgcgggcaaa acccacgcga ttgtcgccgc cgcccgcggc accggggacg ccttcgctgg
     7621 tgcgcaccat ggtgccgccc gcctgcatgc gatagccgtc ggtgtgctgg agccggtcct
     7681 cctcgtcttc gtgatagcgc gcgccgatct ccatctcgtt gcgcgtgccg aagccgtcga
     7741 aggagacgcc tacgatcgac tcgacgccct gcgcgtagta ggtgcggttg ttcgccttga
     7801 ccgcgagcgc gccgtccgcg ctggccgaat cgccggtcag gtgggcgtac tggctcgcgt
     7861 gcgcgtccgg atcggccagc acgtcgtcga gcgacacgcc gtcgacgcga tccagcttgt
     7921 accagttgcg gtcgaagtcg ttgcgataga ggctggtggt gacgtcgacg ttttcgctcg
     7981 ccacaatgaa gtggcgcacg agcccctggg tgtggtcgtt cttcatctgg tcgaactgag
     8041 aggccgcata gcggcgatag gggtcgcgcg cgaagtcgtc gtcggtcagg cccaggtagg
     8101 tctcattgga cgtctcgtcc ttgtagccca gcttgagcgt cacctcttgg tagtgcttgg
     8161 cctccgtcga cgacgcgacg cgcaccttac cgacgtaatc ctcgacgtcg aagccggtgt
     8221 tgccgccggt gtcgagattc ttgaacccgt cggtgcgggc ctggtacgcc tccaggagcc
     8281 acgatacccg cttccacgtg tcgccgtaat tcgcgtacag cttgcgcgtg tcgttggagc
     8341 ccgcggagac gcgcgcattg gcggccgtct gctccgggat cgaggtggac aggagattca
     8401 gcgcgccccc gttggtgcgc gggccgtact tgatctgact cgagcccttg cgcacctcga
     8461 cgccctccat gcggccggtg atggggaagt agtaggcggc gggcgccgcg tacggcgccg
     8521 gagcggccag cacgccgtct tccatcagcg tgatgttggc gctgcggtcg gtgccggctg
     8581 cgcgcatgcc gatattcggg cgcaggccgt agccttcctc ttccgtgatg acgaccccgg
     8641 gcacgcgcag catgacgcga tggatgtcgt cgtaggcctg gttctccttc gcgagctcgg
     8701 tggccgagat gcggtggacg gatcccggga tgccggccgc gcgaccgggg gcacccacca
     8761 cggtgatgcg attgaggagc ttggtttcgg gcgtgtcggc gcgcttctcc ttcgacgaag
     8821 tctcggacgg cggcgcgtcg ctcttttctt ccgtcgcgac cgcgaacgag gggaccacca
     8881 ccaacaggaa tgcgaccaga acgcgcgcga gggcgcgctg cgggatcatg gcgaccatgg
     8941 agtttcttac ctcacgagtc aaagcgttca acgaccaagt tagggacggt taacttcggg
     9001 atcgaaagaa aagcccgcgg ccggcgaaac ggccgggcgg gcgtcctagc cccagtggtc
     9061 gtcgtgacgg gagaggatgt tcgacggagc gaggtcgagc gggatctgga gcagattcgg
     9121 gactacgacg tgcatcgcgg tccacctccg ctctcggagc acgcgctgca cgattcgccc
     9181 ttctcggcgc agtcgagcca gttgccgaag tgcgcgatgg tttcgttggc gccgcggtta
     9241 cacacgtgga ggaactcgag gaacgactcc aagcgctcga gcgtcttggg gctgacgacg
     9301 tgctcgagca cacacgcgtc gcgcgacgcg gtcgcggcgg ggagtcgcaa cacctccagg
     9361 agaaagcgat aaatgaggtc gttgcgatcc ttgacctcgc gcgccacttt gcgaccgcgt
     9421 atggtcagct gcacgtcgcc gtagcggtcg tgcgcgacca gcccgcgctg ctgcagcgtc
     9481 tcgagcgcgc cggtgaccga gggcatcttg acgcccaagc gatccgcgat ggccttgacg
     9541 cgcggctcgg tgccctcgcc gacgatctcg aagatcgtct tgaggtacat ctccatgttg
     9601 ggtgtgagtg gcttgttcat tcggcgtcgc cgggcgcgag ttcgtcgcac ctaactcggg
     9661 cgccactcta gggcatgggc aaggaagccg tcaagccccc gcgcgagatt ttttggtggc
     9721 tacctggccg cgcgcgggtg gcgtccagtg ggctgctgga cgacaggacc cgcgccttgc
     9781 gcaagttatg ccccccgctg cgccgcggcg accggcatcg gctgcttgag cgtcaggacc
     9841 gggcagggcg agcggcgcac cagcgcttcg gccgtgcttc ccatgaggac gtgcgacagg
     9901 cccgtgcgcc catgggtgca ggacacgatc aggtccgcgt tgatctcgct cgcgtacttg
     9961 actacctggt cgaccgcgac gccctcgagc accttaccct cgatgtcgac ggtcttgggc
    10021 aggccggcga ccaccttcgc gagcgcgtcg cggatggcgg cgctgtgctc gcgctggctg
    10081 atcagcggat cgatgcccac gaaggcgacg tccgacacct ggagcgccgg ctcgttgacg
    10141 cacaccacct tgagcttggc cttgaagcgc tcggccaggc cgaccgcgta cgggagcgcc
    10201 cgcagcgaat actcggaaag atccgtggtg acgacgatga cgttgaagtt caacatggtc
    10261 tcctccttga gtcactgctg ggcgacgcgt gcctcgggga cgccgcgcgc cgaccgcttg
    10321 gctttgagca cgttggcggc ttcccgcagc gtcattttgg tcagcgtcgc cgcgtagttg
    10381 ttgcgaaaca gcttccagac gtcgtggagc gcgcagggcg cttcgtcgct gcaggtgtcg
    10441 aggccgagga cgcacaccgt gtcgaggtcg acgcggccgt cgacggcttc gatcacttcg
    10501 atgacacgca tctggtcgcc gggtcgcgcg agctgatatc cgccgcccgg gcccttggtc
    10561 gagcgcacca acccgcgccc gcgaagggaa tgcaggatct tcgcgaggga cgggtggggc
    10621 acatcggccg cttcggcgat ctcgcgcacg agcaccggtc ccggcgcgtc tttctcggcc
    10681 aggtagatca gcgcgcgcag cgcatgctgg caagtcggtg agtgaatcat agcagtaccc
    10741 tttcgctagt agctggtccg ttactccact aactagtcta gtcacccggc ccgggctcgt
    10801 caatggaata tttagagtgt tttatcctgt ataggagact agaccgcacc cgtatgtggg
    10861 acaacgtttt acgcggtcat ggattccccg attgcgcggc cccaggggat tcggctaagc
    10921 tgggcgcctc gtaagccggc cgccttcgtc cggtgtcatt catgccgatc gccacaccct
    10981 tccatccccg aacctccgcg cactgcgtga gcctgttcta taaggactgg gcggggtact
    11041 acgccgtccg ctcctacgac accacccacg accgcgagta cttcgcgttt cggcacgcgt
    11101 gcggattgat cgacgtcacg ccgctctaca agtacgacgt gcgcggcgac gacgcggcgc
    11161 gtttcctcgc tcgcgtcatg gtgcgcgaca ttcgcaagct caaggtgaac caggtctact
    11221 acgtgtgctg gtgcgacgac cacggccgca tgatcgacga cggcacggtc tcgcgcctct
    11281 ccgagaccga ctaccgcgtg acgtcgtccg agccggcgtg gcactggctc cagcgcaccg
    11341 cgcgcggcta ccgcgtctcg atcgaggacg tgagcgacgc gatcgcggcg ctgtccctgc
    11401 aaggaccaac gtcgcgcgat gtgctggacg ccgtcaccga tggcgcagtc gcgaagctgg
    11461 gcttcttcag gctgacgcgc gggaagatcg cgaaggcgga cgtgctcgtg tcgcgcaccg
    11521 gctacaccgg cgacctgggc tacgaaatct ggatggccaa cgccgacgcg ctgcgcgtgt
    11581 acgacgcgct gttctccgcg ggccacgcct acgggctcga gcccgccggg ctcgacgcca
    11641 tggacgtcac ccgtgtcgaa gcgggcttca ttctcaacgg cgtcgac
//
LOCUS       DASPGK010000428         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1040235, whole genome shotgun sequence.
ACCESSION   DASPGK010000428 DASPGK010000000
VERSION     DASPGK010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040235"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VEC56_11910"
     CDS             <1..184
                     /locus_tag="VEC56_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14900.1"
                     /translation="TIDNHGSSADYEVEIPRGVALLEVSIGERRIVIKDGEAFAAGLP
                     LDDRGRSILSLAAPDE"
     gene            268..993
                     /locus_tag="VEC56_11915"
     CDS             268..993
                     /locus_tag="VEC56_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14901.1"
                     /translation="MKLYRVFLLAVAATASITGCNEDRSPSTPTSADPPNTLEEAGGG
                     SMPFSEAQIFIEYNATANDAGIQVFLDAEPWKEIQIVDHRGRELLEIEASGGMKRLGL
                     TELRFEGAEPEPDEVLDAFPAGEYRFRGRAVERFRLTGTATLSHDIPPAPEFSPSQGE
                     VVDPDNVVIEWDAIGGVDRYQVIVESDANGFVLEVSVSSSTTSLHVPPTFLEPNTEYK
                     AEILAIAPSGNRTITEGTFVTGP"
     gene            complement(1064..1420)
                     /locus_tag="VEC56_11920"
     CDS             complement(1064..1420)
                     /locus_tag="VEC56_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HXV14902.1"
                     /translation="MAYVDGFVIPVPKKNLAAYRRIAKQAGRIWKKYGALEYRECVGD
                     DLAVKYGMPFPRLSKLKAGETVVFSWIVYKSKTHRNSVNKKVMTDPAIAKMMKMKPIF
                     DDKRMTYGGFKVMVDM"
     gene            complement(1455..2744)
                     /locus_tag="VEC56_11925"
     CDS             complement(1455..2744)
                     /locus_tag="VEC56_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV14903.1"
                     /translation="MATPAHRAIEAVWRIESPRLIAGLTRIVRDVGLAEDLAQDALVV
                     ALEKWPESGVPDNPGAWLMATAKHRAIDRLRRGQLMERKHEELARDLAQQETTAPDHD
                     AALDDPVGDDLLRLMFIACHPILSTEARVAMTLRLLGGLATDEIARAFLVPESTVAQR
                     IVRAKRTLAEARVPFEIPRGEELRARLSSVLGVIYLVFNEGYSATAGDDWMRPALCED
                     AIRLGRVLAELAPDEAEVHGLVALMEIQASRSGARVGPTGEPILLLDQDRRRWDQLLI
                     RRGLAALDRAEQLNGALGPYTLQASIAACHARAQTAQETDWRRIVALYDALVQLSPSP
                     VVELNRAVAVTMAFGPAAGLELVDALTAEPALKSYHLLPAVRGDTLEKLDRFDEAHKE
                     FVRAASLTRNERERTLLLDRAAACARRSAARGPNDAA"
     gene            complement(2763..3197)
                     /locus_tag="VEC56_11930"
     CDS             complement(2763..3197)
                     /locus_tag="VEC56_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXV14904.1"
                     /translation="MRFMIIVKATPYTEKGNPIAETLKKHPAVFAAMTAYHEDLAKAG
                     ALLDCSGLHPTSKGWRVKYSGNTRTVVDGPFTEAKELIAGYTLFQAKSKEEAMEWVRR
                     FPNPSVDGSEGEIEVRQLFELDEFGESAEIDRFRRIQDESRK"
     gene            complement(3337..>3836)
                     /locus_tag="VEC56_11935"
     CDS             complement(3337..>3836)
                     /locus_tag="VEC56_11935"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXV14905.1"
                     /translation="SRRTRDRGTVIMNEPAASPRKIYPPIYLLLASLLMIGLHKIAPG
                     RQLVDWPERYWGIVPMGIGVWIAILVNAMFRRAGTTIKPFQESSALVISGPFRFSRNP
                     IYLGMAVFLVGLGMLLGSLTPFVVVPAFVLLIDRRFIRAEEAMLARAFGAEYEQYRQR
                     VRRWM"
BASE COUNT          632 a         1289 c         1240 g          675 t
ORIGIN      
        1 gaccatcgac aaccatgggt cgtcggccga ctacgaggtc gagatcccgc gcggcgtcgc
       61 gctcctcgag gtctccatcg gagaacggcg catcgtgatc aaggacggcg aggccttcgc
      121 ggccgggctc ccgctcgacg accgcggccg gagcatcctg tcgctcgcgg cccccgacga
      181 atagccgtcg ccatcgcgtc acatcccgga gccctggcgg gttcctctcc cagtccggct
      241 atacgcctgc caccaggagg aatcgccatg aagctctacc gcgtgtttct gcttgccgtt
      301 gccgccacgg cgtcgatcac gggctgcaac gaggatcgat cgccgtccac acccacgagc
      361 gccgacccgc ccaacacgct cgaagaggcc gggggcggct ccatgccgtt cagcgaggcg
      421 cagatcttca tcgagtacaa cgccaccgcc aacgatgccg gcatccaagt cttcctcgac
      481 gccgagccgt ggaaagaaat ccaaatcgtc gatcatcgcg gaagagagtt gctcgaaatc
      541 gaagcgagcg gcggcatgaa gcgcctcggc ttgaccgagc ttcgtttcga gggcgccgaa
      601 cccgaacccg acgaagtgct ggacgccttc cccgcgggcg agtaccgctt ccgcggccgg
      661 gccgtcgagc gcttccgatt gacgggaacg gcgacgctgt cgcacgacat cccgcccgcg
      721 cccgaattct cgccgtcgca gggcgaggtg gtcgatcccg acaacgtcgt gattgaatgg
      781 gacgcgatcg gcggagtgga tcgctaccaa gtcatcgtcg agagcgacgc gaacggcttc
      841 gtgctggagg tgagtgtgtc gtcgtcgacg accagcctgc acgttccgcc gacgttcctc
      901 gagccgaaca ccgaatacaa ggcggagatt ctggccatcg cgccgagcgg caatcgcacc
      961 atcacggagg ggaccttcgt caccggaccg tagcggccat cgacctccgc cgcgggtcca
     1021 tcggcccggg tcgtgccgac gcgccgaccc gggccgggcg acgctacatg tcgaccatga
     1081 ccttgaagcc gccgtaggtc atgcgcttgt cgtcgaagat cggtttcatc ttcatcatct
     1141 tcgcgatagc cggatcggtc atgaccttct tgttgacgct attgcggtgc gtcttcgact
     1201 tgtagacgat ccacgagaac acgaccgtct ccccggcctt gagcttgctc aggcgcggaa
     1261 agggcatgcc gtatttgacc gcgaggtcgt cgcccacgca ctcgcggtat tcgagcgcgc
     1321 cgtacttctt ccagatccgg ccggcctgtt tggcgatccg gcggtaggcg gcgaggttct
     1381 tcttgggaac gggaatcacg aatccatcga catacgccat cgttgtcctc cttgccttgg
     1441 tttcgcgtgt gggttcacgc cgcgtcgttc ggtccgcggg cggccgatcg gcgcgcgcaa
     1501 gcggcggcgc gatcgaggag cagcgtgcgc tcgcgctcgt tccgcgtcag ggacgccgcg
     1561 cgcacgaact ccttgtgcgc ctcgtcgaag cgatcgagtt tctcgagtgt atcacctcgc
     1621 accgcgggca ataggtggta actcttgagc gccggctcgg cggtgagtgc gtcgaccagc
     1681 tcgaggcccg ccgcaggacc gaacgccatc gtcactgcga ccgcacgatt gagctccacc
     1741 accggcgacg gcgagagctg cacgagcgcg tcgtacagcg cgacgatccg cctccagtcc
     1801 gtctcttgcg cggtctgcgc ccgcgcgtgg cacgcggcga tggacgcctg gagcgtgtac
     1861 ggccccagcg caccgttgag ttgttccgcg cgatccaacg cggccaggcc gcggcgaatg
     1921 agcagctggt cccatcgcct gcgatcttgg tcgagtaaca ggatcggttc gccggtgggc
     1981 ccgacgcgcg cgccggagcg cgaggcttgt atctccatca acgcgaccag accgtgcacc
     2041 tccgcctcgt cgggggcgag ctcggccagc acgcgaccga gccggatcgc gtcctcgcac
     2101 agcgcgggcc gcatccagtc gtcgccggcg gtggccgagt agccctcgtt gaagacgagg
     2161 tagatcacgc cgagcaccga cgacaggcgc gcgcgcagct cttcgccgcg cgggatctcg
     2221 aaggggacgc gcgcctcggc gagcgtgcgc ttcgcgcgca cgatgcgctg cgccacggtc
     2281 gactcgggga ccaggaacgc gcgcgcgatc tcgtcggtgg caagacctcc gagcagtcgg
     2341 agggtcatcg ccacccgcgc ctccgtggag agaatggggt ggcacgcgat gaacatcagg
     2401 cgcaggagat cgtcgccgac cggatcgtcg agcgccgcgt cgtggtcggg tgcggtcgtt
     2461 tcctgctgcg cgaggtcgcg tgccagctcc tcgtgcttgc gctccatcaa ttggcctcgg
     2521 cgaagccggt cgatcgcgcg gtgcttggcg gtggccatga gccaggcgcc cgggttgtcc
     2581 ggcacgcccg actccggcca cttctcgagt gccaccacga gcgcgtcttg cgccaaatcc
     2641 tcggccaggc cgacgtcgcg cacgatgcgg gtcagcccgg cgatgagtct cggcgactcg
     2701 atcctccaga ccgcctcgat ggcgcgatga gccggcgtcg ccatggttcg gttgtgcccc
     2761 gattacttcc tcgactcgtc ctggattctg cggaaacgat cgatctccgc gctttcgccg
     2821 aactcgtcca gctcgaacag ctgccggacc tcgatttcgc cttcgctgcc gtcgacggaa
     2881 gggttcggga agcgccgcac ccattccatc gcctcttctt ttgacttggc ctggaagagg
     2941 gtgtagccgg ctatcaattc cttggcctcg gtgaaggggc cgtcgacgac cgtgcgcgtg
     3001 ttgcccgagt acttcacgcg ccagcccttc gaggtcgggt gaagaccgga gcagtcgagc
     3061 aatgctcccg ccttcgcgag atcttcgtgg tacgccgtca tcgcggcgaa taccgcgggg
     3121 tgcttcttga gggtttcggc gatgggattg cccttctcgg tatagggcgt cgccttgacg
     3181 atgatcatga accgcatggt gttgcttcct ttcggttttc ggatgctctc cctgccatca
     3241 cgacgaacgg ggggtctcga gatcgacacg gccggcgctt ttttcaccga gctcccaacc
     3301 cgttggccgt cggtcgcttg gagcgctgcg cgcgattcac atccagcgcc ggacgcgctg
     3361 gcggtattgc tcgtactcgg cgccgaaggc tcgggccagc atcgcttctt cggcccgaat
     3421 gaagcgccgg tcgatgagga gcacgaacgc gggaacgacc acgaacggtg tcaacgatcc
     3481 gagcagcatg cccagcccga ccaggaaaac cgccatcccc aaataaatcg ggttgcgcga
     3541 gaagcgaaag ggcccgctta tcacgagcgc cgaagattct tggaacggct tgatggtcgt
     3601 cccggcgcgg cgaaacatcg cgttgaccag gatcgcgatc cacacgccga tccccatcgg
     3661 cacgatcccc cagtaccgct ccggccagtc gaccagctgc ctgcccggcg cgatcttgtg
     3721 gagcccgatc atcaagagcg aagcgagcag gagatagatc ggtggataga ttttgcgggg
     3781 acttgcggca ggttcattca tgatgacggt tccccgatca cgcgtccgac gacttc
//
LOCUS       DASPGK010000429         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1510503, whole genome shotgun sequence.
ACCESSION   DASPGK010000429 DASPGK010000000
VERSION     DASPGK010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510503"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VEC56_11940"
     CDS             complement(<1..207)
                     /locus_tag="VEC56_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14906.1"
                     /translation="MLDEVRGTSGSETQLSHAKGGAGGRSPAYALFEHLDAEQAAVEA
                     TGRPAGAMLIPGLEATITTFEEGQI"
     gene            complement(345..1727)
                     /gene="cmk"
                     /locus_tag="VEC56_11945"
     CDS             complement(345..1727)
                     /gene="cmk"
                     /locus_tag="VEC56_11945"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXV14907.1"
                     /translation="MSRASRSTPRTAEELLRTFSISIDGPSGSGKTTTARLVARELGL
                     RHVDTGAMYRAATLIALERGIDLGDGERLGAVANDLEIFVEQDGRGEPCVRVGERDVT
                     DAVRSPQVTAAVSAVSAHPAVRAAMVRRQRELSEQGGVILEGRDIGSVVLPWADVKIY
                     LDATVRVRAQRRHAELSRGGVQTTIDEVERDLVRRDTLDASRADSPLVRPIGAWLVDT
                     SQATIEGEVARVVSLARAAAAERAASFASREPRQRMRLPYHAVILIVRSLYFVLFGLR
                     WKSLLETEPAQNYLFASNHISYFDPPAVSITLPREVHFVAKDSLFKIWWLGPLIRYLN
                     AFPIKRGVFDREAMATALSLLESGQSVLIFPEGGRIAGGELGAARSGVGYLAVLSGVP
                     VVPVYLAGTDRLKDCLFRKSRVRVIHGKPIRIPAGLIEEYRSQDDRAIYRRHSDMVMA
                     AVQALKERRR"
     gene            complement(1724..>2385)
                     /locus_tag="VEC56_11950"
     CDS             complement(1724..>2385)
                     /locus_tag="VEC56_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV14908.1"
                     /translation="IVFVCNPNNPTSTYVSASEVSALLAGVPDDVLVVMDEAYIDYVD
                     APDYPDSLQIRRTRENVVVLRTFSKFFAVAGVRVGYAIAHPLVVDAIHKVRQPFNVSR
                     LAQAAGLGALACANELKPYAQETIAERARVRDAIVSLGLVCPASQANFVFVDLGASTL
                     DLFAELSQRGVVVRRLGQFGSARNTYRITVGTPPENDRLIEALRAVIHHRPAASRPAS
                     S"
BASE COUNT          406 a          810 c          815 g          354 t
ORIGIN      
        1 gatctgacct tcctcgaagg tcgtgatggt cgcttcgagt cccggaatga gcatggctcc
       61 cgccgggcgg ccggtcgctt caacggctgc ttgctcggca tcgaggtgtt cgaacagcgc
      121 gtacgccggc gaacgccccc ccgcacctcc cttcgcgtgg gatagttggg tctccgaccc
      181 agaggttccg cgaacttcgt cgagcatcaa accgtgtctc ctcctgtgaa ctgaacggac
      241 gacacccgcg ccatgcgagt ggccgtggaa accggcatca taccggctcc aaggggggct
      301 tgcaaccgga atctagggaa ggacttcggg gcgtgggccg aggcctaccg gcgccgctcc
      361 ttcaaggcct ggacggcggc catgaccatg tcgctgtgac ggcgatagat ggcccggtcg
      421 tcctgggagc ggtactcctc gatcagcccg gccgggatgc ggatcggctt gccgtggatc
      481 acgcggacgc gggacttacg aaacaagcag tccttcaagc ggtccgtgcc cgcgagatag
      541 accggcacca ccgggacgcc ggagagcacg gcgaggtagc cgacaccgct gcgcgccgct
      601 cccagttccc cgccggcgat gcggccgcct tcgggaaaga tgagcaccga ctggccgctt
      661 tcgaggagcg acaaggcggt cgccatggcc tcgcggtcga acacgccgcg cttgatggga
      721 aatgcgttca agtaccgaat caacgggccc agccaccaga tcttgaacag cgagtccttg
      781 gccacgaagt gcacctcgcg cgggagcgtg atgctgacgg cgggagggtc gaagtacgag
      841 atgtggttcg acgcgaacaa atagttctgc gcgggctcgg tctcgagcag actcttccag
      901 cgcaggccga acaacacgaa gtacaaggac cgcacgatga gaatcacggc gtgatacggc
      961 aggcgcatcc gttgccgagg ttcccggctc gcgaacgagg ccgcgcgctc ggcggccgcg
     1021 gcgcgagcga gcgacaccac gcgtgcaact tcgccttcga tggtggcctg cgaggtgtcg
     1081 accagccacg cgccgatcgg ccgcaccagg gggctgtcgg cgcgcgaggc gtccagcgtg
     1141 tcgcgccgta cgaggtctcg ctctacctcg tcgatggtgg tctgtactcc gccgcgcgac
     1201 agctccgcat ggcggcgctg cgcgcgcacg cgcacggtgg cgtcgaggta gatcttcacg
     1261 tcggcccacg gcaacaccac gctgccgatg tcgcgcccct cgagaatgac gccaccctgt
     1321 tcggagagct cgcgctgtcg ccggaccatc gcggcgcgca ccgcggggtg cgcgctcacc
     1381 gccgagaccg cggccgtcac ctggggcgat cgcaccgcgt cggtgacatc acgctcgccc
     1441 acgcgaacgc acggctcgcc gcgcccgtct tgctcgacga agatctccag gtcgttcgcc
     1501 accgcgccca ggcgctcacc gtcgccgagg tcgatccccc gctccagcgc gatcagcgtc
     1561 gccgcgcggt acatggcgcc ggtgtcgacg tggcggagcc cgagctcccg cgcgaccaaa
     1621 cgcgcagtcg tggtcttgcc ggaccccgac ggcccgtcga tcgagatgga gaaggtgcgg
     1681 agcagctcct ccgccgtgcg cggtgtcgag cgggaggcgc ggctcaactc gaagcgggcc
     1741 gcgacgcggc gggtcggtgg tggatgacgg cgcgcagcgc ttcgatcagg cggtcgttct
     1801 cggggggtgt cccgacggtg atgcggtagg tgttacgcgc ggacccgaac tggcccaggc
     1861 gccgcacgac cacgccgcgc tgcgacaact cggcgaacaa gtcgagagtc gaggcgccca
     1921 ggtcgacgaa cacgaagttg gcctgggacg cgggacacac gagtcccagc gacacgatgg
     1981 cgtcgcgcac ccgcgcgcgc tccgcgatgg tctcttgggc gtagggcttc agctcgttcg
     2041 cgcacgccag cgcgcccaac ccggccgcct gcgcgaggcg gctcacgttg aagggctggc
     2101 gcaccttgtg gatcgcgtcc accaccagcg ggtgcgcgat cgcgtagccc acccgcacgc
     2161 cggcgacggc gaagaacttc gaaaaggtgc gcaggaccac cacgttctcg cgcgtgcggc
     2221 gaatctgcaa cgaatccgga taatcaggcg cgtcgacata gtcgatatac gcctcgtcca
     2281 tcactaccaa tacgtcgtcg ggaaccccgg cgagaagagc gctcacttcg ctcgcgctta
     2341 cataggtgga agtcgggttg ttggggttgc acacgaacac gatgc
//
LOCUS       DASPGK010000430        11066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_223201, whole genome shotgun sequence.
ACCESSION   DASPGK010000430 DASPGK010000000
VERSION     DASPGK010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223201"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..639
                     /gene="groES"
                     /locus_tag="VEC56_11955"
     CDS             355..639
                     /gene="groES"
                     /locus_tag="VEC56_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXV14909.1"
                     /translation="MKMIPLGDRVIVRLLEQEEKKKGGIIIPDTAKERPQEGEVVAVG
                     KGRRNTAGERIEPEVKKGDRVLFAKYAGNEIEIESTKYLVVKEDEILAIL"
     gene            679..2301
                     /gene="groL"
                     /locus_tag="VEC56_11960"
     CDS             679..2301
                     /gene="groL"
                     /locus_tag="VEC56_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXV14910.1"
                     /translation="MPKQLIYDAEAREKLRAGVDKLANAVRITLGPKGRNVVLDKKFG
                     SPTVTNDGVTIAKEIELEDPYENLGAQMVKEVATKTQDVAGDGTTTATILAQAIIKEG
                     IKNVTAGANPMYIKKGIQKAVDAVVEELKTKSKKVKDDEIGSVATISANNDPEIGKLI
                     ADAFAKVGKDGVITVEEARGMETTLDVVEGMQFDRGYLSPYFVTNAERMEVELDEAVI
                     LIYDKKISSMKDLLPILEKVAQMGRPMLIIAEDVEGEALATLVVNKLRGTLQVCAVKA
                     PGFGDRRKAMLEDIAILTNGRVISEEAGFKLENAQLADLGKAKRITIDKDNTTVVEGA
                     GKPADIKARVTQIRRQIDETTSEYDKEKLQERLAKLAGGVAVINVGAATEVEMKEKKA
                     RVEDAKEATRAAMEEGIVQGGGIALLRASKVLDKVKEAGDAAIGVEIIRRALEAPLRQ
                     IALNAGAEGSLIVEHVLKQVDAIGYNAATGEYVDMFKAGIVDPTKVTRTALQNAASVA
                     AMLLTTEAVVTDIPAEEKAPARGAGGGMGDMY"
     gene            2617..4272
                     /locus_tag="VEC56_11965"
     CDS             2617..4272
                     /locus_tag="VEC56_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14911.1"
                     /translation="MKTIDRPKRRWLLGAALVATFGLFMGACGNDEEGSPTDVTNSQP
                     IQITDIIASPKSADPGDTLLLSAIVTSTSPNVGDVPSMEWTATGGAFLEDNQTSVRWI
                     APASGVYTVTARARNTVSSATGSADLFVGGSATVVTSLAGAITLKANQSDFYYLRTGN
                     NVVTQGTDVWQVVSGTAGDAVTLPAATTGTNSRRVAYAPDLSFQVHSSDSIVIGLTTS
                     PVHLYLGDFATSTYRRISSGTPSGDRYPGFTDAAVAPDNHSIAFGAMLPTPLAAGADT
                     FDIVVYDNAGPTRRNLTAAHTNHRNVFPTWSTDQRWLAFISDRSGRGQWDLYGMPINT
                     LGVINTAQASLVRLSNTSGRLADGDPGQLTFVSPPYEWNPAVPTLAVRDADGILHLII
                     TTPAGATQIDVGAVNGFAWSPDGSLLALALPGLVATIGSDGTGFVYRVGRDDDGNATR
                     PDDNFNDISWSPDGGWIVYRATRASSSWLEVYDVDQSTIVDPIALTPAEPTASLAWSL
                     PVYRVLMSMKPAWGTAGVMYYPTFGTGAATVGIRSVDVSGLTP"
     gene            4289..4660
                     /gene="folB"
                     /locus_tag="VEC56_11970"
     CDS             4289..4660
                     /gene="folB"
                     /locus_tag="VEC56_11970"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXV14912.1"
                     /translation="MADIIRLKDITVFGFYGVSPAEREVGQKIQIDLDVHTDLSRACR
                     SDSLHDTINYESIYSKVIEIVGGEKRYRLLEALGDEICAAMIANFPVSRVEIRLRKLN
                     LPFPNNLSHVEVVLVREAPLI"
     gene            4664..5173
                     /gene="folK"
                     /locus_tag="VEC56_11975"
     CDS             4664..5173
                     /gene="folK"
                     /locus_tag="VEC56_11975"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXV14913.1"
                     /translation="MGTRAFIALGSNLGRREETILRALRTLEARGAANVVAASSLYES
                     DAVGMGAAPAFVNAVAEVVPLLRPRDLLERMQAIEAELGRTGGHNQSRTIDLDLVAYG
                     DEVSDAEGLSIPHPRFRERAFVLVPLSEIAPDFRDPRTHAPMGQLVAALADAASVRRV
                     SSRRWIVQG"
     gene            5188..5889
                     /locus_tag="VEC56_11980"
     CDS             5188..5889
                     /locus_tag="VEC56_11980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXV14914.1"
                     /translation="MADQRTMDKSYAEIRRGILARGYARVAVEGNIGVGKSSLARLIV
                     KRLGGQLVEDRVDDNPFLERFYEDSGAYAFQTQLVFLMNRYKQQLALNQRDLFSDFVV
                     FDYVFARDRIFANVNLGDDELALYERVATELEAKVVAPDLAIYLQASAPVLFDRIQSR
                     GHAFERSLSRDYLEALNEAFNHYFFNYSAGPLLVVNTDAMDFVNDERHFVDLMTRLTE
                     PVVNTEFYVPSFGTR"
     gene            5897..6712
                     /gene="panB"
                     /locus_tag="VEC56_11985"
     CDS             5897..6712
                     /gene="panB"
                     /locus_tag="VEC56_11985"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXV14915.1"
                     /translation="MTPRPKVTVQTLLDMKRRGERIAMLTAYDYATALIEDRAGVDVM
                     LVGDSVGMAVLGYENTIPVTVEEIVHHLRAVARSRPRAMVIGDLPFMSYQAGVADAVR
                     SAGRLVKEGNAQAVKLEGGRRMEPVIKAIVDAAIPVMGHLGLTPQSLHQLGGYRVQGK
                     EPRDADRLVEDATLLETCGCFAIVVEGMPWQVAKRITESIAIPTIGIGAGPHCDGQVL
                     VVNDLLGLSEEFAPRFVKRYAEVGKQMASAFQAYIDDVKAGRFPDLDHSYSTE"
     gene            6716..7615
                     /gene="panC"
                     /locus_tag="VEC56_11990"
     CDS             6716..7615
                     /gene="panC"
                     /locus_tag="VEC56_11990"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXV14916.1"
                     /translation="MEVIRSVEPLARYLDGLGAGERISFVPTMGALHEGHGACVARAR
                     SVARARVLVSIFVNPTQFGPGEDVARYPRPLDADLARLDAWGCHAAFVPSVDTMYPTP
                     QRAWVEVDEIAQPLCGRFRPGHFRGVATVVSKLFHMVRADAAVFGQKDAQQALVIRAM
                     VRGLDFPVRLLLAGTVREPDGLAMSSRNAYLSPGERTQASALYRALGAGRASLEAGER
                     DARAIEGAAADVLRRAGIDRVDYAEVRRADDLSALDRVEGRAILALAAWVGRARLIDN
                     MVFDVESNRVASDVALFDDDARE"
     gene            7629..8387
                     /locus_tag="VEC56_11995"
     CDS             7629..8387
                     /locus_tag="VEC56_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXV14917.1"
                     /translation="MQRVRDIAVVILAAGQGKRMQSDLPKVLHEINARPMIRFVIDAV
                     QALGPDRVVVVVGHQAERVEAACRGTGVSFARQTRQLGTGHAVMQALPLLSGYAGTIV
                     VLNGDVPGLKTETIRRFIEFHRASGFAATVLTARLPDPGGYGRIVRDAAGRLQRIVEH
                     KDASESERAIDEINSGLFCFEAADLAASIGRVGRGNAQNEYYLTDVIGVLASAGRPVG
                     AYCVDDSREVAGVNDLGELEAARRFVGGAPGAAP"
     gene            8384..8869
                     /locus_tag="VEC56_12000"
     CDS             8384..8869
                     /locus_tag="VEC56_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXV14918.1"
                     /translation="MMDRLYAPWRAAYFMTEKDDGCLFCKLVSSTDDRAHMILARGRD
                     WFVVVNRYPYTTGHVMVVCNRHVEKMGDFRGREGEEMVELVGRCERAIARVYAPDGVN
                     LGANLGRSAGAGVVGHFHLHVVPRWQGDTNFMSAVGETRVVSEDLNDTYDKLHRALQE
                     S"
     gene            8899..9348
                     /locus_tag="VEC56_12005"
     CDS             8899..9348
                     /locus_tag="VEC56_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HXV14919.1"
                     /translation="MARRKPVRKRGRKRASIGQRIAVALSTVVLVLCAASISFSVFVR
                     KTGDDGKARPLRVELLNGTGVSGIAESARGALAKLGVDVVSVGNAERFDYSESILVAR
                     ERGTDVRLLGEMIRCRHVVDQLDKAAAADASLILGADYRSLALGWPD"
     gene            9422..9694
                     /locus_tag="VEC56_12010"
     CDS             9422..9694
                     /locus_tag="VEC56_12010"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HXV14920.1"
                     /translation="MAPRRRPLALVTLILFLGIVVGTVLGEAIGVILPEGKVIRDVFV
                     NSTDFHVGPVHVDLVVFSFTFGFSLRVNMMSVLGVFVVFGFLRYYW"
     gene            9709..9918
                     /gene="tatA"
                     /locus_tag="VEC56_12015"
     CDS             9709..9918
                     /gene="tatA"
                     /locus_tag="VEC56_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXV14921.1"
                     /translation="MVFAFLGPIGWNELVIILVIVLIIFGPRRLPDFAEAFGKSIRKF
                     KQATREAREEIESSTDKKTDKPPTE"
     gene            complement(9992..>11066)
                     /locus_tag="VEC56_12020"
     CDS             complement(9992..>11066)
                     /locus_tag="VEC56_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXV14922.1"
                     /translation="VVRIPIAATEADRAELVYGAELVREQAVKGDFAEIAKAYSESHT
                     AAVGGETGFIGAAQRDPAVMAAIASLKPGDVSPVTSTPDGVAIVQLVATKKERGETLY
                     NIREIVMKLAPGPATIDSLSALAQDIQQAAEENRDLAAAANARGREVVTSQPFAKGMP
                     IPGVGYVPALSRFAFAAEIGAVSGVVADENNFYVARLQNRTPAAARPLTEVAQAIKST
                     LEREAKTESARRKARAFLRSAVTPRTTFREVAQQYGYDVAKTDSFSVSTPVADLPPYS
                     TFARAALSGQPDDTVGPIASGNAVYVIHVTGRRDPEPARMSALIPGTRERLYQQKVQA
                     YVLYWFAHLKETSKIEDLREVSS"
BASE COUNT         1920 a         3553 c         3770 g         1823 t
ORIGIN      
        1 ggcgggccgg ggtccgctcg gcgcgtttcc tgaccggcgg gtacgtggac cacgtgaaca
       61 ggtggctccc gaacaggtgg acgcggtcgc cggacttgac tctcctgggg ttggcccgga
      121 tcgcccgtgt tgggacaacg cgaatgccta caaagagata gagccgactc cggtcgccgg
      181 ccaggggatc actcgacccc ggcaagtaaa gtctccttac gaaatttttc acttgccgtg
      241 ccttaaatca tcatgtaatc tcctcccagt tagcagtcgc ggccagcgac tgccaaatca
      301 actcatcatc agcacaccgc tttggtcgat aaccggaacg aaggaggcag ggacatgaag
      361 atgattccac tcggtgaccg cgtgatcgtg cggctcctgg agcaggaaga gaagaagaag
      421 ggcggcatca tcatccccga caccgcgaag gagcgcccgc aagagggcga agtggtcgcg
      481 gtcggcaagg ggcgtcgcaa caccgccggc gagcgcatcg agcccgaagt caagaagggc
      541 gaccgcgttc tcttcgccaa gtacgccggc aacgagatcg agatcgagag caccaagtac
      601 ctcgtcgtga aagaagacga gatcctggcg attctctagt ttccatccac ccaacatcca
      661 ctatcaggag gcaatgaaat gccgaagcag ttgatctacg acgccgaagc ccgcgaaaag
      721 cttcgtgccg gcgtcgacaa gctcgccaac gccgtgcgca tcacgctcgg ccccaagggg
      781 cgcaacgtcg tcctcgacaa gaaattcggt agcccgaccg tgaccaacga cggcgtgacg
      841 atcgcgaagg agatcgagct cgaggatccg tacgagaacc tgggcgcgca gatggtgaaa
      901 gaagtcgcca ccaagacgca ggacgtcgcg ggcgacggca ccaccaccgc gaccatcctc
      961 gcgcaggcga tcatcaagga aggcatcaag aacgtgaccg cgggcgccaa cccgatgtac
     1021 atcaagaagg gcatccagaa ggcggtcgac gccgtcgtgg aagagctcaa gaccaagagc
     1081 aagaaggtca aggacgacga gatcggtagc gtcgcgacca tcagtgccaa caacgacccc
     1141 gagatcggca agctcatcgc cgacgccttc gccaaggtgg gcaaggacgg cgtcatcacg
     1201 gtcgaagagg cgcgcgggat ggagaccaca ctcgacgtgg tcgagggcat gcagttcgat
     1261 cgcggctacc tctctccgta cttcgtgacc aacgccgagc gcatggaagt ggagctcgac
     1321 gaggccgtca ttctcatcta cgacaagaag atctcgtcga tgaaggacct gctcccgatc
     1381 ttggagaagg tcgcgcagat gggccgtccc atgctgatca tcgccgagga cgtcgagggc
     1441 gaagcgctcg cgacgctggt ggtcaacaag ctacgcggta cgctgcaggt gtgcgcggtc
     1501 aaggcacccg gcttcggcga tcgccgcaag gccatgctcg aggacattgc gatcctgacc
     1561 aacggtcgtg tcatctccga ggaagcgggc ttcaagctcg agaacgcgca gctcgccgac
     1621 ctcggcaagg ccaagcgcat caccatcgac aaggacaaca ccacggtggt cgagggtgcg
     1681 ggcaagccgg ccgacatcaa ggcgcgcgtg acgcagatcc gtcgtcagat cgacgagacg
     1741 acgtcggagt acgacaagga gaagctgcag gagcgcctgg cgaagttggc cggcggtgtt
     1801 gcggtgatca atgtcggcgc cgcgaccgaa gtggaaatga aggagaagaa ggcgcgcgtc
     1861 gaagacgcca aggaagcgac ccgcgcggcc atggaagagg gcatcgtgca gggcggcggt
     1921 atcgcgctct tgcgcgcgtc caaggtgctc gacaaggtca aggaggccgg cgatgcggcc
     1981 atcggggtcg agatcattcg tcgtgcgctg gaagcgccgc tgcgccagat cgcgttgaac
     2041 gccggcgcgg agggctcgct catcgtcgag cacgtgctca agcaggtgga cgcgatcggt
     2101 tacaacgcgg ctaccggcga gtacgtcgac atgttcaagg ccggtatcgt ggacccgacc
     2161 aaggtcacgc gaaccgcgct gcagaacgcg gccagcgtcg cggccatgct gctcaccacc
     2221 gaggcggtcg tcaccgacat cccggcggaa gagaaggccc cggcgcgcgg tgcgggcggc
     2281 ggcatggggg acatgtacta agccgatcgc gagcggcacg caagcgactg gtgcgaacgc
     2341 gccgtcgggg caacccggcg gcgcgttttg cttccgctac cccgagcgtc cccggcgggt
     2401 gtcgcgactg gggcccccgc gtcctcggac acggggtgag gcgcgacagg acccgcgggg
     2461 cgggtcgccc gcccagaatc cccttgaaac gcacgcgttg gacggatatc gtagggccag
     2521 gtttacccca cccgagtgat tatttcaggg ttacagaatc gggcgagccc ctcggccgag
     2581 ttcgtgatgc ttggtcctgt tgcggaggtt tagcatttga agacgatcga tcgaccgaag
     2641 cggagatggc tgctcggcgc ggccctggtg gcgacgttcg gactattcat gggggcctgt
     2701 ggcaacgacg aagaaggttc gcccaccgac gtcacgaact cccagcctat ccagatcacg
     2761 gacatcatcg cctcgccgaa gtcggcggac ccgggcgata ccctcttgct ctcggccatc
     2821 gtgacctcga cgtcgcccaa cgtgggcgac gtgccgagca tggagtggac cgcgacgggc
     2881 ggcgcatttc tcgaggacaa ccaaaccagt gtgcgctgga tcgcgccggc ctcgggcgtg
     2941 tacacggtga ccgcgcgcgc gcgcaatacg gtgagttccg ccaccggttc ggccgacctc
     3001 ttcgtgggcg gcagcgcgac cgtcgttacg agcttggcgg gagcaatcac gttgaaggcg
     3061 aatcagagcg atttttatta cttgcgaacc ggcaacaacg tcgtcaccca aggcaccgac
     3121 gtgtggcagg tagtctcggg aaccgccggt gacgcggtca cgctgcccgc cgccaccacc
     3181 ggcaccaaca gccgtcgggt cgcgtatgcg cccgatttga gcttccaggt tcattcgtcg
     3241 gactcgatcg tcatcggact gaccacgagt ccggtgcatc tctatctggg cgacttcgcc
     3301 acctcgacct accggcgcat ttcgagcgga acaccgagcg gtgaccgtta tcccggcttc
     3361 accgacgcag ctgtcgcacc cgacaatcac agcatcgcgt ttggcgcgat gctgccgacg
     3421 ccgctggccg ccggcgccga taccttcgac atcgtcgtct acgacaatgc aggaccgacg
     3481 cgacggaatc tgaccgcggc gcataccaat catcgcaatg ttttccccac ctggtccacc
     3541 gatcagcgct ggttggcatt catctcggac cgcagcggtc gcggacagtg ggatctctac
     3601 gggatgccga tcaacaccct tggcgtcatc aacaccgccc aggcgagcct ggtgaggctc
     3661 tcgaacacga gcggccgcct ggccgacggc gaccccggcc aattgacgtt cgtcagtccg
     3721 ccgtacgagt ggaatcccgc cgtgcccacg ctcgcggtgc gagacgcgga cggcatcctg
     3781 cacctgatta tcacgactcc ggcgggcgcc acccagatcg acgtcggcgc ggtgaacggt
     3841 tttgcgtggt ctccggacgg aagtttgctc gcgctggcgc tgccggggct cgtagccacc
     3901 atcggcagcg acgggacggg attcgtatac cgcgtgggca gggacgacga cgggaacgcg
     3961 acgcgacccg acgacaattt caacgacatc tcgtggtcgc ccgacggcgg ctggattgtg
     4021 taccgtgcaa cgcgcgcgtc atcctcgtgg ctcgaggtgt acgacgtcga ccagtcgacg
     4081 atcgtggatc cgatcgcgct gacaccggct gagcccacgg cgtcgctggc ctggagcctg
     4141 cccgtctatc gcgtgctgat gtcgatgaag ccggcctggg ggacggccgg agtgatgtac
     4201 tacccgacct tcggcaccgg agcggcgacg gtgggaattc gctccgtgga cgtgtcgggg
     4261 ctcacgccct agctcgaaag ccgacgccgt ggccgacatc atccggctca aggacattac
     4321 cgttttcggt ttctacggcg tctcgcccgc ggagcgcgag gtcggacaga agatccagat
     4381 cgacctcgac gttcataccg acttatcgcg cgcgtgccgt tccgactcgc tgcacgacac
     4441 gatcaactac gaatcgatct atagcaaagt gatcgagatc gtgggcggcg agaagcgata
     4501 ccgcctgctc gaggccctgg gcgacgagat ctgcgcggcc atgatcgcga acttccccgt
     4561 gagccgcgtc gagattcgcc tgcgcaagct caacctgccg ttccccaaca acctctcgca
     4621 cgtcgaagtg gttctggtgc gcgaagcgcc gctgatctga tcagtgggca cacgcgcctt
     4681 catcgcactt ggctccaacc tcgggcggcg cgaagagacc atcctgcgcg cgctgcggac
     4741 actcgaagcg cgcggtgcgg ccaacgtggt ggccgcgagc agcctatacg agtccgacgc
     4801 cgtcggcatg ggagccgcgc ccgcgttcgt caacgcggtc gcggaagttg ttcctttact
     4861 tcgacctcgc gatttgttag agcggatgca ggcgatcgaa gccgagctcg ggcgaaccgg
     4921 cggccacaac caatcgcgga cgatcgacct ggatttggtg gcctacgggg acgaggtcag
     4981 cgacgcggag gggctctcga tcccgcaccc gcgcttccgc gagcgagcgt tcgtcctggt
     5041 accgctctcc gagatcgcgc ccgattttcg ggacccgcgg acgcacgcgc cgatgggcca
     5101 actggtggcg gcgctcgccg acgcggcctc ggttcgccgg gtgtcgagcc ggcggtggat
     5161 cgtccagggc tgacctcgag gccgaatgtg gccgaccagc gaacgatgga caagagctac
     5221 gccgaaatcc gtcgcggaat cctcgctcgc gggtacgccc gggtggcggt ggagggcaac
     5281 atcggggttg gcaagtcgtc gctcgcgcgc ttgatcgtga agcggctcgg cggacagttg
     5341 gtggaggacc gcgtcgacga caatcccttc ctggagcgct tctacgagga ctcgggcgcc
     5401 tacgccttcc agacccagct cgtgtttctc atgaaccgct acaagcagca gctggcgttg
     5461 aaccagcgcg acctgttctc cgacttcgtc gtgttcgatt acgtcttcgc ccgcgaccgc
     5521 atcttcgcca acgtcaacct gggcgacgac gagctggcgc tctacgagcg cgtcgccacc
     5581 gagctggagg ccaaggtggt cgcccccgat ctcgcgatct acctgcaggc gtcggcgccg
     5641 gttctgttcg accgcatcca gtcgcgcggc cacgccttcg agcgatcgct ctcgcgcgac
     5701 tatctcgagg cgctcaacga ggcgttcaat cactacttct tcaactactc ggcggggccg
     5761 ctcctggtcg tcaacaccga cgcgatggac ttcgtcaacg acgagcggca cttcgtcgac
     5821 ctcatgacgc ggctgacgga gccggtcgtc aacaccgagt tctacgtgcc gtcgttcggc
     5881 acgcggtagg tttcgcatga caccccgccc caaggtcacg gttcaaaccc tgctcgacat
     5941 gaaacggcgc ggcgagcgca tcgcgatgct caccgcgtac gactacgcca ccgcgctgat
     6001 cgaggatcgc gccggggtcg acgtgatgct ggtgggggac agcgtgggca tggccgtgct
     6061 cggctacgag aacaccatcc ccgtgacggt cgaagaaatc gtccatcatt tgcgcgcggt
     6121 ggcgcgctcg cgcccgcgcg cgatggtgat cggagatctg ccgttcatga gctaccaggc
     6181 gggtgtcgcg gacgcagtgc gcagcgcggg ccggctggtc aaggaaggca acgcgcaggc
     6241 ggtcaagctc gagggcggcc ggcgcatgga accggtcatc aaggccatcg tcgacgcggc
     6301 cattccggtc atgggacacc tgggactcac gccgcagtcg ttgcaccagc tgggcggtta
     6361 ccgcgtgcag ggcaaggagc cgcgcgacgc ggaccgcctg gtcgaagacg cgacgctgct
     6421 cgagacgtgc gggtgcttcg caatcgtcgt cgaagggatg ccgtggcagg tggccaagcg
     6481 catcaccgag tcgatcgcga ttcccaccat cggtatcggc gcgggaccgc actgcgacgg
     6541 ccaggttctg gtcgtcaacg acctgctcgg actgagcgaa gagttcgcgc cgcgcttcgt
     6601 gaagcgttac gccgaggtcg gcaagcagat ggccagcgcc ttccaggcct acatcgacga
     6661 cgtcaaggcg ggtcgctttc ccgacctgga ccacagctac tccacggaat agcgcatgga
     6721 agtgattcgt tccgtcgagc cgctcgcgcg ctacctcgat gggctcggcg ccggcgagcg
     6781 gatttcattc gtacccacca tgggtgcgct gcacgaggga cacggcgcgt gcgtcgcgcg
     6841 ggcccgctcg gtcgcgcgcg cccgcgtgct cgtctcgatc ttcgtcaacc ccacccaatt
     6901 tggacccggc gaagacgtcg cgcgttaccc gcgcccgctc gacgccgacc tcgcccggct
     6961 ggacgcgtgg ggctgccacg ccgccttcgt cccctccgtc gacacgatgt atcccacgcc
     7021 gcagcgtgcg tgggtcgagg tcgacgagat cgcgcaacca ttgtgcggcc gttttcgtcc
     7081 cgggcacttc cgcggcgtgg ccacggtggt gtcgaagttg ttccacatgg tgcgcgcgga
     7141 cgcggccgtg ttcggacaga aggacgcgca gcaggcgctc gtgattcgcg cgatggtgcg
     7201 cgggctcgac tttccggtgc ggctcctgtt ggctggtacc gtgcgcgagc ccgacggctt
     7261 ggcgatgagt tcgcgcaacg cgtacctgtc gccgggcgag cggacgcagg cgtcggcgct
     7321 gtaccgcgcg ctcggggccg gacgcgcgtc gttggaggcg ggcgagcggg acgcgcgcgc
     7381 gatcgagggc gccgccgcgg acgtgctgcg ccgcgccggt atcgatcgcg tcgactacgc
     7441 ggaggtgcgg cgcgcggacg atctttccgc tttggaccgc gtggaaggcc gggctatact
     7501 ggccctcgcc gcgtgggtgg gacgcgcgcg gctcatcgac aacatggtgt tcgacgtcga
     7561 gagcaaccga gtcgcttccg acgtggcgct gttcgacgac gacgcgcggg agtgagggag
     7621 gcgtgaccgt gcagcgagtt cgtgacatcg cggtggtgat cctggcggcg ggccagggca
     7681 agcgcatgca gtccgatttg cccaaggtcc tgcacgagat caacgctcgt ccgatgattc
     7741 gcttcgtgat cgacgcggtg caggcgttgg gacccgaccg cgtcgtggtc gtggtcggcc
     7801 accaggcgga gcgcgtggag gcggcctgcc gcggcaccgg ggtctccttc gcgcgtcaga
     7861 cccggcagct cggcaccggc cacgcggtga tgcaggcgct tccgctgctc tcgggctacg
     7921 cggggacgat cgtggtgctc aacggcgacg tgcccggcct gaagaccgag accattcgcc
     7981 gcttcatcga gttccaccgc gcgtccggat tcgcggccac cgtgctcacg gccaggctcc
     8041 ccgaccccgg cggttacggg cgcatcgtgc gcgacgccgc cggccgtctc cagcgtatcg
     8101 tcgagcacaa ggacgcgagc gagagcgagc gcgcgatcga cgagatcaat tcggggctgt
     8161 tctgcttcga ggccgccgac ctggccgcgt cgatcgggcg cgtcgggcgc ggcaacgcgc
     8221 agaacgagta ctacctcacc gacgtcatcg gcgtgctcgc gtcggccggg agaccggtgg
     8281 gcgcgtactg cgtcgacgac tcgcgcgagg tcgcgggcgt caacgacctg ggcgagctgg
     8341 aagcggcgcg ccgcttcgtg ggcggcgccc ccggcgcagc gccatgatgg accgcctgta
     8401 cgcgccgtgg agggcggcgt acttcatgac cgagaaagac gacggctgcc tgttctgcaa
     8461 gttggtgtcg tcgacggacg accgcgcgca catgatcctg gcgcgcgggc gggactggtt
     8521 cgtggtcgtc aatcgatacc cgtacacgac cggacacgtg atggtggtgt gcaaccggca
     8581 cgtggagaag atgggcgact ttcggggccg cgaaggcgag gagatggtgg aactggtggg
     8641 gcgctgcgag cgcgccatcg cgagggtgta cgcgcccgac ggcgtcaatc tcggcgccaa
     8701 cctgggacgc agtgccgggg ccggagtggt cggacacttc cacctgcacg tggttccgcg
     8761 ctggcagggg gacaccaatt tcatgtcggc ggtgggcgag acgcgcgtgg tctcggaaga
     8821 cttgaacgat acctacgaca agctgcaccg cgcgttgcag gagagctgag tcggccctta
     8881 ggcgctgaaa ggaagacgat ggcacgcagg aaaccagtcc gcaagcgggg gcgcaagcgc
     8941 gcgagcatcg ggcagcgcat tgcggtggcg ctttccaccg tcgtgctggt gctgtgcgcg
     9001 gcctcgatca gcttctcggt gttcgtgcgc aagaccggcg acgacggcaa ggcgcgcccg
     9061 ctgcgcgtgg agctcttgaa cggcaccggg gtgtccggga tcgcggaatc ggcgcgcggc
     9121 gcgctcgcga aactgggcgt cgacgtggtg tcggtgggga acgcggagcg cttcgattac
     9181 agcgagtcga tcctggtcgc gcgcgagcgc gggaccgacg tgcggctgct cggcgagatg
     9241 atacgctgcc gtcacgtggt cgatcagctc gacaaggcgg cggccgcgga cgcctcgctc
     9301 atcctcggcg ccgactaccg cagcctggcc ctgggatggc cggattagca cttggcgggc
     9361 cgcggcggtt cccctatgct cggccggtcg tgtcaatgtc gatgacgtcg aggaggaagc
     9421 cgtggcaccg cgccgccgac cgctagcgtt ggtcacgctg attctgtttc tcgggatcgt
     9481 cgtgggaacg gttctgggtg aagcaatcgg tgttatactt cccgagggaa aggtgatccg
     9541 cgacgtgttc gtcaacagca ccgactttca cgtcggcccg gtgcacgtcg acttggtcgt
     9601 gtttagcttc accttcgggt tctccctgcg cgtcaacatg atgagcgtgc tcggagtctt
     9661 cgtcgtcttt ggtttcttgc gctactactg gtagcgcgcg gaggagtcat ggtgttcgcg
     9721 ttcttgggac cgatcggctg gaacgagctg gtcattatcc tcgtcatcgt cttgattatc
     9781 tttggcccgc ggcgccttcc cgatttcgcg gaagcattcg gcaagtcgat ccgaaagttc
     9841 aagcaggcca ctcgcgaggc gcgcgaggag atcgagtcgt cgacggataa gaagaccgac
     9901 aagccgccca cggaatgaaa acgggcccgg ccgaggccgg gcccgcaggt aactcgccgt
     9961 tgtgcccccg tatcggggcg ccgcccactc gctacgaaga aacctcgcgt agatcctcga
    10021 tcttgctcgt ctccttgagg tgagcgaacc agtagagaac gtacgcctgt accttctgtt
    10081 ggtagagcct ctcgcgcgtg cccgggatca acgccgacat ccgcgccggc tcggggtcgc
    10141 ggcgtccggt gacgtggatc acgtacacgg cgttgccgct cgcgatcggg ccgaccgtgt
    10201 cgtcgggctg gcccgacaag gcggcgcgcg cgaaggtgct gtacggtggc aggtcggcca
    10261 ccggagtcga caccgagaac gagtcggtct tggcgacgtc gtaaccatac tgctgggcca
    10321 cttcgcggaa cgtggtccgg ggcgtgaccg cggagcgaag gaatgcccgc gccttgcggc
    10381 gcgccgattc cgtcttggcc tcgcgctcga gggtcgactt gatggcctgg gcgacttcgg
    10441 tcaaggggcg cgcagcggcc ggggtgcggt tctggaggcg cgcgacgtag aagttgtttt
    10501 cgtcggcgac cacgccgctg acggcgccga tttccgccgc gaacgcaaag cgcgagagcg
    10561 ccggaacgta tcccacgccc ggaatcggca tccccttcgc gaagggctgg ctggtcacga
    10621 cctcgcgccc gcgcgcgttc gccgccgccg ccaagtcccg gttctcctcc gcggcttgct
    10681 ggatgtcctg cgcgagtgcc gaaagagaat cgatggtcgc gggaccgggt gcgagcttca
    10741 tcacgatctc gcggatgttg tacaacgtct cgccccgctc cttcttggtc gccaccaact
    10801 gaacgatcgc cacgccgtcg ggagtcgacg tcaccgggct cacgtcgccg ggcttgagcg
    10861 aggcgatggc cgccatcacg gccgggtcgc gctgggccgc gccgatgaat cccgtctcgc
    10921 ctccgaccgc cgcggtgtgc gactccgagt acgctttcgc gatctcggcg aagtcgccct
    10981 tgaccgcctg ctcgcgaacc agctccgcgc cgtacacgag ctccgcgcgg tcggcttcgg
    11041 tggccgcgat gggaatgcgc acgacg
//
LOCUS       DASPGK010000431         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_575020, whole genome shotgun sequence.
ACCESSION   DASPGK010000431 DASPGK010000000
VERSION     DASPGK010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575020"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VEC56_12025"
     CDS             complement(<1..672)
                     /locus_tag="VEC56_12025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV14923.1"
                     /translation="MRDAFFVMSGGIGLPAMRARLFLPGIVVLAAVLRFWHIGAQSLW
                     IDEYLTLEVATAKPGYPIWQLLLHNIHGPLHALSVFLFGLIGRSDGWLRLPSALAGIA
                     TVPLLFLWMRPRFGQRAALWGALLLAINPLHVHYSQELRNYAFAVLFVMAGAVCVDGL
                     IARATRARAAAFAVCAAAAVLSNFSATFSYAAHAVSYFKRAGVTRTTVARFAAISLLL
                     VVLISP"
     gene            746..1717
                     /locus_tag="VEC56_12030"
     CDS             746..1717
                     /locus_tag="VEC56_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXV14924.1"
                     /translation="MSLESRLHIGPAGWQYDDWYGTMYPSPRPRGFDPLAYVASYFNL
                     IEINSTFYRVPAPATCRRWVERVEIHPRFLFTAKAHQSFTHRDDASPSDRAEFVNAMS
                     VLHDGGRLGAILLQYPWSFRFDASSRRRLDALVADLKALPLALEVRHASWESEQAQAY
                     LAEQPLCVCGIDQPVIGESLRPYHYRPGPAGAYFRLHGRNYRNWFAADAGRDARYDYL
                     YSRTELSPWTEVIRAAARASERVFVVLNNHFRGQAPANAFELAAMLLGRSLPAPATLR
                     RAYPALVASTTHPDDAGETGSLFESPPSGPEEARARPRPRRPRSRPR"
     gene            complement(1653..3368)
                     /locus_tag="VEC56_12035"
     CDS             complement(1653..3368)
                     /locus_tag="VEC56_12035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV14925.1"
                     /translation="MQAVLRIAGYDSFEQIAVGGMAVVFKARKLSVKKTVAIKVLLPH
                     LAADARFITRFQQEAEAAARIQHDNIVNVIDSGKSDGAYFIVMEYYDGLTVEELLSTQ
                     ARLPIDVALSVVLNVCYGLEAAHAQNLVHRDIKPANIIFTRQGGIKVADFGLAKAIDK
                     LTLVTHAGKVVGTPAYMSPEQTRGEAVGTASDIFSLGVVAYEMLAAKRPFEGASYSEV
                     VDRIQHVEPQAVASFNPLVEEPFEQIVRRMLAKPLNARYAHASEIVMDLEEAMDKHGL
                     RRDRRGLEEFFKDPAGYTQKSNTALLARLHGETPRGPDRNRGVGVRHYRKILFLNPDD
                     EAARAALEKMGENASAGSESPAMGEPVRRAPSMPAADPNADYRVILETIDRSIETPDT
                     FALKLSIRLKSPVVRMRQLVSRAPVTMAQRLPYKKARWLMKVIEELGGTARLEVVVDA
                     APVVDEKPAPATSREIAKPLERRSPSGGILCPRCGWEEEPDARFCSLCLQHFNKTDKI
                     DAPNFATRPGFDDPNENPLSEGEATRAAPAPAPSPGLPRRPVLIAGAIGVAAVVVLLV
                     LLLGR"
     gene            complement(3506..3898)
                     /locus_tag="VEC56_12040"
     CDS             complement(3506..3898)
                     /locus_tag="VEC56_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14926.1"
                     /translation="MHFSRSTHRLSFRAAALALPILLLGVAGCPKQEDFPVALDLDVP
                     PTPFNFVISNAGTNYTFAWEVSDPAAVDHYLVYLLGGGLAPNEVIIETALTSVTYDVG
                     FSLAGLQFAVSAVSAQGVEGEWTVRAAQ"
     gene            complement(3910..4452)
                     /locus_tag="VEC56_12045"
     CDS             complement(3910..4452)
                     /locus_tag="VEC56_12045"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HXV14927.1"
                     /translation="MIRGKTLRGIAAALAAVFVISLAAPASAADSLLLSILLPGLGQA
                     QDGHYAKASVFGSAALISWTGLFATQLNYSRTVDRYENQKRNYLAYQDQLENGQTVYI
                     GDINDTYTAMSNAYNQAEDDEKVRNIFIGALIVTYGLNIADILLTGEDTGEVDRAPSA
                     PSTSLEWRGGGLHLVRTIRF"
     gene            4639..>5186
                     /locus_tag="VEC56_12050"
     CDS             4639..>5186
                     /locus_tag="VEC56_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV14928.1"
                     /translation="MIDVHALSKRYGSRDAVREVSFSVGVGEIVGFLGPNGAGKTTTL
                     RMLTGFLPATSGRARVAGFDVVTQSMQVRARIGYLPESVPLYRDLTVSAYLEFVGVLK
                     GVARAERARRIGFVVDACGIGEVRGRRIGTLSRGFRQRVGLAQALLNDPDVLFLDEPT
                     VGLDPKQIVEIRELIRSLAGRRT"
BASE COUNT          888 a         1687 c         1734 g          877 t
ORIGIN      
        1 gggcgagatc aacaccacga ggagcagtga aatcgccgcg aatcgtgcca cggtggtacg
       61 cgtgacgccg gcccgcttga agtacgagac ggcgtgtgcg gcgtaggaaa acgtggccga
      121 aaaattcgac agcacggctg ctgccgcgca cactgcgaag gcggccgcgc gcgcccgcgt
      181 ggcgcgcgcg atcaagccat cgacgcacac cgcgccggcc atgacgaaca ggaccgcgaa
      241 ggcatagttg cgaagctctt gcgagtagtg cacgtgcaaa gggttgatcg cgaggagcag
      301 cgcgccccat agcgctgccc tctggccgaa gcggggtcgc atccacagga acaacagcgg
      361 aacggtggcg atgcccgcga gtgcgctggg aagacgcagc cagccgtcgc ttcgaccaat
      421 cagcccgaac aggaacaccg aaagggcgtg cagcgggccg tggatgttgt gcaggaggag
      481 ctgccagatg ggatagcccg gcttcgcggt cgcgacctcg agggtgaggt actcgtcgat
      541 ccagaggctt tgggcgccga tgtgccagaa cctgagcacc gcggccagca cgacgattcc
      601 cgggaggaag aggcgcgcgc gcatcgctgg taagccgatt cctccgctca tgacgaaaaa
      661 cgcgtcgcgc acgcgacgcg tgctatactt ctttcccgcg tgaggcgtgt cgcgtgcatc
      721 gacacgcgct tgggaggaac gatcggtgtc cctggaatcc cggcttcata tcggtcccgc
      781 gggatggcag tacgacgact ggtacggaac catgtatccg tccccgcgtc cgcgcggctt
      841 cgacccgctc gcatacgtcg catcgtactt caacctcatc gaaatcaata gcaccttcta
      901 ccgcgttccg gcacccgcga cgtgccggcg ctgggtcgag cgcgtggaga tccacccgcg
      961 cttcctattc accgccaagg cgcaccagtc gttcacgcat cgcgacgacg cgagtccgtc
     1021 cgaccgcgcc gagttcgtga acgcgatgtc ggtactgcac gacggcggac gcttaggcgc
     1081 catcctgctc cagtacccgt ggtcgtttcg attcgatgcg tcgtcacggc gccggctgga
     1141 tgcgctggtc gccgacttga aggcgctccc cctcgcgctc gaggtgcgcc acgcgtcctg
     1201 ggagtcggag caggcgcagg cctacttggc cgaacagccg ctgtgcgtgt gcggcatcga
     1261 ccaacccgtc atcggtgagt ccctgcggcc gtaccactac cgcccgggcc cggccggcgc
     1321 atactttcgc cttcacggcc gcaactaccg gaactggttc gccgccgatg cgggccgcga
     1381 cgcgcgttac gactacttgt actcgcggac agagctctct ccctggaccg aggtgatccg
     1441 cgccgcggcg cgtgcgtcgg agcgcgtgtt cgtcgtattg aacaatcatt tccgcggtca
     1501 agcgcccgcc aatgccttcg agctggcggc gatgctcctg ggacgttcgc tcccggcacc
     1561 ggccacgttg cggcgcgcgt accccgcgct cgtcgcgagt acgacgcacc ccgacgacgc
     1621 gggagaaacc ggcagcttgt tcgagagtcc gcctagcggc ccagaagaag cacgagcaag
     1681 accacgaccg cggcgacccc gatcgcgccc gcgatgagga ccgggcggcg cgggaggccg
     1741 gggcttggcg cgggcgccgg cgcggcccgg gtcgcttcac cctccgacag cggattctcg
     1801 ttggggtcgt cgaacccggg tcgggtggcg aagttgggcg cgtcgatctt gtcggtcttg
     1861 ttgaagtgct ggagacacag cgagcagaag cgagcgtcgg gctcttcttc ccatccgcag
     1921 cgcgggcaca gaatgccgcc gctgggcgag cgccgctcga gcggtttcgc gatctccctc
     1981 gaggtcgccg gcgcgggctt ctcatcgacc accggggccg cgtcgaccac gacctccagg
     2041 cgagcggtac cgcccaactc ctcgatcact ttcatgagcc agcgcgcctt cttgtacggg
     2101 aggcgctgtg ccatcgtgac cggcgcccgt gacaccagtt gacgcatgcg caccaccggc
     2161 gacttgagcc gaatcgacaa cttgagcgca aatgtgtccg gcgtttcgat cgagcgatcg
     2221 atggtctcga gaatcacgcg atagtccgcg ttggggtcgg cggcgggcat gctgggcgca
     2281 cgccgcacgg gctcgcccat ggccgggctc tcgctgcccg cgcttgcgtt ctcgcccatc
     2341 ttctccaggg ctgcgcgcgc cgcctcgtcg tccggattca gaaagaggat cttgcgatag
     2401 tgtcgcacgc cgacgccgcg attgcggtcg ggaccgcgcg gcgtctcgcc gtggagacgc
     2461 gcgagcaacg ccgtgttcga tttctgggtg tagccggccg ggtccttgaa gaactcctcc
     2521 aacccgcgcc ggtcgcgtcg caggccgtgc ttgtccatgg cctcttccag atccattacg
     2581 atctcggacg catgcgcgta acgggcgttg agaggcttgg ccagcatgcg gcgcacgatc
     2641 tgctcgaatg gctcctcgac cagcggattg aacgacgcaa ccgcctgcgg ttccacgtgc
     2701 tggatgcggt cgacgacctc cgagtagctg gccccttcga agggacgctt ggccgccagc
     2761 atctcgtagg cgacgacacc cagcgagaag atgtcgctcg ccgtacccac cgcctcgccc
     2821 cgggtttgct cgggcgacat gtaggccggc gtacccacca ccttgccggc gtgggtgacc
     2881 agggtgagct tatcgatcgc ctttgcgagt ccgaagtccg cgaccttgat gccgccttga
     2941 cgcgtgaaga tgatgttcgc gggcttgatg tcgcggtgga cgaggttctg cgcgtgcgcc
     3001 gcctcgaggc cgtagcacac gttgagcacg accgagagtg cgacgtcgat gggcaagcgc
     3061 gcttgcgtgc tcaggagttc ctcgacggtc aagccgtcgt agtactccat gacgatgaag
     3121 taggcgccgt ccgacttccc cgaatcgatc acattgacga tgttgtcgtg ctggatgcgg
     3181 gcggcggcct ccgcttcctg ctggaagcgc gtgatgaagc gggcatccgc cgccaggtgc
     3241 ggcaggagga ccttgatcgc gaccgtcttc ttgaccgaga gcttgcgcgc cttgaagacg
     3301 accgccatcc ccccgacggc gatctgctcg aaggaatcgt acccggcgat gcgtaggacg
     3361 gcttgcatgg tgcggtcccg gcacagggac cgcgcgagga gtgcagggat cgtaccacgc
     3421 gcgccgcggg gggcggacgc ggttcatggc agaggcgagg tgcctcgcat aacagactgc
     3481 aaaaacgact cttagagagg tttagctact gggccgcacg gacggtccat tcgccctcga
     3541 cgccctgagc gctgaccgcg gagaccgcga actgcaggcc ggccagcgaa aacccgacgt
     3601 cgtaggtgac cgaggtcagg gccgtctcga tgatgacctc gttgggagcc aggccgcccc
     3661 cgaggagata gaccagataa tgatcgaccg ccgcggggtc gctgacttcc cacgcgaagg
     3721 tgtaattcgt gcccgcgtta ctgataacga agttgaaggg ggtcggcgga acgtccaggt
     3781 cgagcgcgac cggaaagtcc tcctgtttgg gacacccagc cactcccaac agcagaatcg
     3841 gaagtgcaag agccgcggcc cggaatgaca gacgatgtgt ggaacgagaa aaatgcatgc
     3901 gtcgcactcc tagaaacgaa tcgttcgcac caggtgaagg ccgccgccac gccactcgag
     3961 cgaggtgctg ggggcgctcg gcgcgcggtc cacttcgccg gtgtcttcgc cggtcaacag
     4021 aatgtcggca atgttgagcc cataggtgac aatgagggcc ccgatgaaga tgttgcgcac
     4081 cttctcgtcg tcctcggcct ggttgtacgc gttgctcatg gcggtgtagg tgtcgttgat
     4141 gtcgccgatg tagacggtct gcccgttttc gagctggtcc tggtaggcca ggtaatttcg
     4201 cttctggttc tcgtagcggt cgaccgttcg cgagtagttg agttgcgtcg cgaacagacc
     4261 cgtccatgaa atcagtgctg ccgacccgaa gacggatgcc ttcgcgtagt gaccgtcctg
     4321 ggcctggccg aggccgggca agaggatcga caagaggagc gaatccgccg ccgaggcggg
     4381 tgccgcgagg gaaataacaa aaaccgcggc cagagcggcc gcgatcccac gtaacgtctt
     4441 gccacgaatc atggatacgc cttacgtttc tacgagttga atcgatctag gcggttaccg
     4501 gccgccccga ctgtctacgc ggtcgagcgg tgaatgttac gtgcaaaccc ccaatgaggc
     4561 aatttttttc tccggcgcgc ccacccggcg cgcggccgcc ttcgacacgc gcgtccagcg
     4621 cgagcccggc gaatccgcat gatcgacgtc cacgctctaa gtaagcgcta cgggtcgcgc
     4681 gacgcggtgc gcgaggtctc gttctcggtc ggggtgggcg agatcgtggg attcctggga
     4741 cccaacggcg ccggcaagac cacgaccctg cgcatgctga ccggattcct gccggccacg
     4801 tcggggcggg cgcgcgtggc cgggttcgac gtcgtcacac aatcgatgca ggtgcgcgcg
     4861 cgcatcgggt acctccccga atcggtaccg ctctatcgcg acctcaccgt gtccgcgtac
     4921 ctcgagttcg tgggcgtgct caagggtgtc gcgcgcgccg agcgcgcgcg acgcatcggc
     4981 ttcgtcgtcg acgcgtgcgg catcggcgag gtgcggggcc gccgcatcgg aaccctctcg
     5041 cggggattcc gccagcgcgt gggactggcg caggcgctcc tgaacgaccc cgacgtgctc
     5101 ttcctggacg aacccacggt gggcctcgac cccaagcaga tcgtcgagat tcgcgagttg
     5161 atccgcagcc tggccggccg gcgcac
//
LOCUS       DASPGK010000432        14416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_106777, whole genome shotgun sequence.
ACCESSION   DASPGK010000432 DASPGK010000000
VERSION     DASPGK010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106777"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..602
                     /locus_tag="VEC56_12055"
     CDS             48..602
                     /locus_tag="VEC56_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV14929.1"
                     /translation="MKRLVVPALVALVLLPACSQKGFDGSVKITPQPVAGVDFAAYKT
                     WMSGRESYPATGLEHIDQPQFRVAVARHFVEEMTKLGYTNSTENPDFVILMHVAAEQK
                     FDEQKMDDVYKGYDMAWSQISDDDVWNEGTIIIFAMDAKTGQQVWSSTAQARLQDYVG
                     FQDRLDRFNKVITKMLADFPPRAQ"
     gene            679..1242
                     /locus_tag="VEC56_12060"
     CDS             679..1242
                     /locus_tag="VEC56_12060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV14930.1"
                     /translation="MVGADEVLKRARFVTSYLDHLQLDVRRVLDAGCGTGLWKRALQR
                     IDRDIEYVGIDPSEYLCQRYGWTPASIDAFTSRRKFDLVVCQDVLQYADDDTVGRSFA
                     SIARVCRGALYFDVPTRDDIDDGLLDMRRTDRAIHVRSARWYRARLAKDFVNVGGGVF
                     LRRGARAVALALERWDSAPPRVRRKRD"
     gene            complement(1268..2113)
                     /locus_tag="VEC56_12065"
     CDS             complement(1268..2113)
                     /locus_tag="VEC56_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXV14931.1"
                     /translation="MPEKNERDRTASIAVFCDFENIAIGVRESRYAEFDISRVLERLL
                     LKGSIVVKKAYCDWDRYKDFKRPMHEAAFEMIEIPHVRQSGKNSADIRMVVDALDLCY
                     TKPHVNTFAVMSGDSDFSSLVTKLRENNKIVIGVGVKNSTSDLFTTACDEFIFYDDLV
                     REHEKRRKSRKRAPAKAKAASAPRATAGEADTEERRQKAFDLVLDTIDMLWDERGDED
                     EIWGSMVKQAIKRRQPSFNESYYGFRGFNDLLKSMEQEGLIDIEPDERSGGYIIRDVT
                     TPPAA"
     gene            2391..4298
                     /locus_tag="VEC56_12070"
     CDS             2391..4298
                     /locus_tag="VEC56_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXV14932.1"
                     /translation="MRDRSVYEHISLIAFLAWVGLGADGLSSSSYGPEEAFKALGDHT
                     YLAVGLALLTAITVFVISAAYNGIIELFPHGGGGYFVATKLLGKRVGAVSGSALVVDY
                     VLTITISIAAAGDALFSLFPIEYQVWKLPVEIFLIIVLLLLNLRGVRESVLTLTPIFL
                     LFIVTHIILIVGAIFLHASEMPATAREVQTGFSNGLATLGAGGLALLFIHAYSLGGGT
                     YTGIEAVSNGLAIMREPRAQTGKRTMLYMSISLALTAAGLILCYLLWDIAHVEGKTMN
                     AVLSTAFAGNSTMGQGFTILTLVSEGALLVVAAQAGFVGGPRVLANMAVDSWMPHRFA
                     ALSDRLVTGNGVILMCIASMAALLYTRGDVSHIVVMYSINVFVTFSLSMLGMLLYTLR
                     TKKASRRSKRRVLLFSFGLLLCATILVITTLEKFGEGGWVTLLVTGGVVALCFGIRRH
                     YESVSRKVAKLYESLIEVPRAPDAPDEPLEPKRSDSVAAVLVGDYGGVGLHTCLSVFS
                     KFPGHFKGVVFVSVGVVDSKEFKGEGTVDALRDHLEEDLQRYVAFARKQGIPATYRYS
                     IDTEAVEGAERLCLEVAGEFPQIMFFAGKVIFGKEQWFHPLLHNETAYAIQRRLQWAG
                     QMVVVVPIRVI"
     gene            4491..5069
                     /locus_tag="VEC56_12075"
     CDS             4491..5069
                     /locus_tag="VEC56_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXV14933.1"
                     /translation="MVAIAVVILLVFVAIAGVSGYNRVIKLDQGVAQAWSQVENVYQR
                     RADLIPNLVETVKGAANFEQETLTRVIEARAAATQIKITPEMLNDPQSLQRFEQAQGE
                     LSSALSRLLVTVEQYPELKANANYLALQDELAGTENRIANERRRFTETVQTYNTAVKS
                     FPMNMLAGMFGFAPKAYFEAKEGAEEAPKVEF"
     gene            5075..5860
                     /locus_tag="VEC56_12080"
     CDS             5075..5860
                     /locus_tag="VEC56_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXV14934.1"
                     /translation="MRTSRVTLPRRARLVALATPALVLLAALASAAPSVRVRLPEAPS
                     RFVYDEAGALSSDERRVLEDTLMVYDRRGLEIGVAIFKSLQGDPIEDVSMALAEKWKP
                     GSAERDNGALIVVGMEERSVRIEVGYGLEAVVNDAMAGRIIRNDIAPAFREGRYGLGL
                     SRAVSSLARLARGETLPEPANAELPAWLPLVFILIFILVFISVSRRARRGWISRQGWT
                     GGGWRGPYIGGGGFGGFGGGGSGGGGGSFGGGSFGGGGASGRW"
     gene            5863..6543
                     /locus_tag="VEC56_12085"
     CDS             5863..6543
                     /locus_tag="VEC56_12085"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXV14935.1"
                     /translation="MNGPASSRALRAVAAFLAAAGVVLAILVAAVVMREIVAPPAHTW
                     SAWTRGAVFAMSLVAIVFCVLGGFLSYRAHRARRDSVRTLLTSEERAQVAAAIRDAEA
                     KTSGEVVVHLAERTHHEPTVEARKAFERIGMARTRERNGVLFFVSVRDHRFAVIGDKG
                     IHTHVAPDFWTGVVTHVESRFAQRRFGDGLAEGIATVAAELARYFPRRTDDVNELPDA
                     MSDDAGDD"
     gene            complement(6540..7952)
                     /locus_tag="VEC56_12090"
     CDS             complement(6540..7952)
                     /locus_tag="VEC56_12090"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXV14936.1"
                     /translation="MRTLVWIRGKDLRVSDHPPLLDAASRGEVIPLFVLDPFFFAPAR
                     AQEIPHRIQFLLESLRELSGSIAALGSRLILVEGKSVDVVPRLAAEWNVDRVVAHRWV
                     EPFARERDRRVADALRVPFVLYEGEMLLPPETLRTSAGEPYSVFTPFARAFHATASIA
                     KPLPAPRALPPLPPGVDAPRIPIPTLESVGIVRNDALLAGGEGAARARLDAFLSGPAA
                     HYHTARDRMDRAGTSRLSQDLKFGTLSARTVWTAIDSELGESHAARVFKNELVWREFA
                     HSTLWDRPDLLMRPFRPGFEGFPWVRNEAHWRAWTEGRTGYPVVDAAARQLLGEGFVH
                     NRARMIAAGFLTKHLRVDYRRGEAHYMKYLTDGDWAQNNFGWQWSAGCGCDAQAYFRV
                     FNPIIQGGKFDPEGAYVRRWVTELARVPAKWIHRPWEAPAPLLRHAGVVLGENYPRPI
                     VDHSAAREAFLAAANRHLRT"
     gene            8035..10704
                     /locus_tag="VEC56_12095"
     CDS             8035..10704
                     /locus_tag="VEC56_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV14937.1"
                     /translation="MSLANGTRLGPYEILGTLGAGGMGEVYRARDTRLDRMVAIKVLP
                     AHLVSQPEVRQRFEREARAVSSLNHPNICVLHDVGADFFVMEYLEGESLASRIEKGPL
                     PTAELLQVGIQVADALSAAHRAGLIHRDLKPGNVMLTKSGAKLLDFGLARSTGLEGPT
                     DATQSPTMSRPLTKAGTIVGTFQYMAPEQLEGGEADARTDIFALGAVLYEMATGQRAF
                     SGTSQASLIASILKEQPRAMADLAPMTPPALEHLVKRCLAKDPDDRWQSARDVGLELA
                     WIRDAGSSAGISAPVAAHRRRAARTAWIAAAVCAAAAIGFGSLLLTHRSAPPMPIRFT
                     VEPPANYGFVIGQPHVAVSPDGSALAYCATDTVGVAHVFVRRLDSHESIRINGTQGAS
                     MPFFSPDGAHLAYFSAGKLRRVSIADGVAQALCDAPDPRGGTWSRNDDIVFQPAASGP
                     IVRVSARGGETRVVTEVDTANGDATHRFPMFLPDGKRFVFVVLPPRDQLFDVRVGSLD
                     GETSPVIARMAGAPVYSRSGYLLSFRDEHIIAAAFDARSLEVEGEPVPIGEMAGVGGG
                     YSGAGGATVSENDVLVRTFAGTPNTTVQWLGRDGRPRGRITVPSGFFVEPVFSPDGRR
                     MTLVEWRPDGAANIWMFDVERDVATRFTFENSENFDAMWSPDGKSIVFTSNREGRENL
                     YIKPSDGSREEEKLFDSGELFTKTEAWAPDGSGIVFSVLTEKTNSDLWLFPMTGDRVP
                     RPLLQTRFNEGDAAISPDGRWIAYRSDDAGRAEIYVRSFPDMGPKHRASSEERGVHGV
                     FQAFYAIHWVAGGRELVYLAGDGSTLLSVGVSGGATLTLAPPRPLCRLPLGHAAYAAH
                     PDGQRLIVCGPDVTGTPFGVTVVMNWPALLGRR"
     gene            10801..12399
                     /locus_tag="VEC56_12100"
     CDS             10801..12399
                     /locus_tag="VEC56_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXV14938.1"
                     /translation="MHYLLPHLLTRAASQSPDQIAVVAGSDSVSYRELDRISDRLAAV
                     LSSHGVARGDRVGIFLPKSMGAIVGIYGIMKAGAVYVPVDPAAPPGRVAYIVENCGIR
                     VLLTSAKKAAAVEEILSKGAALDRLILLDDSAWSDGPAVPVVTWTDVLGHDGNAPVLE
                     SIETDLAYILYTSGSTGVPKGVMISHRNSLTFVDWAHDAVGATSEDRFSSHAPLHFDL
                     SVFDLFVACKAAATLVLVPDGLSSFPFRLAEWIEKERISVWYSVPTILSLLVRSGDLG
                     RFRYPHLRTIIFAGEVFPVKYLRDLMALVPKATYFNFYGPTETNVITYYRVPPIPEDR
                     TAPIPIGIACANTEVFAIADDGKRVSRTGEVGELYARGSLVAQGYWGDAEKTQRHFVA
                     NPLSRGFRETAYRTGDIVTLDDDGNYVFLGRRDDMVKSRGYRIELGEVETTLYRHEGV
                     REAAVVALPDPSIGNRLVAFVVLSDEAAADAEDLHEFCGEHLPRYMVPESIELRDHLP
                     KTSTGKVDKVELARRAGGTAPAGN"
     gene            12426..12671
                     /locus_tag="VEC56_12105"
     CDS             12426..12671
                     /locus_tag="VEC56_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXV14939.1"
                     /translation="MDAETSIRRFIVRNLMMKKDENVISLDEPLLEKGIVDSWGIVEL
                     TEFLKKEFGADLDDADILPENFHSVRTIAALVNRGKS"
     gene            12683..13828
                     /locus_tag="VEC56_12110"
     CDS             12683..13828
                     /locus_tag="VEC56_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV14940.1"
                     /translation="MDFSLTQEQTELRDAVIRFARRELSDDVIRRDKDGAFSREHWKK
                     CAEFGIQGMPFPSAYGGQGMDLLATILALEAVGYACKDNGLGFGLGAQMWSVQTPINE
                     FGTEEQKRRYLTKLCSGEWIGAHAMSEPDSGSDSHAMNTLAVRDGDHYVLNGSKTFVS
                     SAPLADVFVTFATLGKDKGFLGITGFVVERETPGVRVSNEIEKMGLRTDPMAQVFFED
                     CRVPVGNRLGRDGQGAAIFRSSMEWERGCIFAPCVGAMQRQLEGCVEHARTRNQFGQP
                     IGKFQAVANRLVDMRMRWEASRWLLYQMAWTKQRDGKAATESAIAKLHISESWVKNCL
                     DAIQVHGGYGYTTEYELERDLRDAVGGTLYSGTSEIQRNLIARQLGL"
     gene            13831..>14416
                     /locus_tag="VEC56_12115"
     CDS             13831..>14416
                     /locus_tag="VEC56_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HXV14941.1"
                     /translation="MCGILAYYNLRGQSIERSQDDIVGLRDMMCHRGPDAFGYAEARD
                     RSWALAHRRLSIIDLSENGRQPMSDTSGRVHLCYNGEIYNYRELKDGLSKRGHRFASE
                     SDSEVILLLYKEMGVDCLPLLRGMFALVIVDEARGCMLIARDPVGKKPLYYGVVGDNV
                     IVASEPGVISRDRGYRKAVDPAGLYSLLTMGAVKS"
BASE COUNT         2355 a         4721 c         4830 g         2510 t
ORIGIN      
        1 ggtttcgtgc tatatctcga cctccaacgt tcgatggagg tcgatcgatg aagcgactgg
       61 ttgtcccggc cctcgtggcc ctcgttctac tgcccgcttg ctcccagaaa ggtttcgacg
      121 gcagcgtcaa gatcacgccg cagccggtgg ccggcgtcga ctttgcggcg tacaaaacgt
      181 ggatgtcggg acgcgagagc taccccgcca ccggactcga gcacatagac cagccgcaat
      241 ttcgtgtggc cgtagccagg cacttcgtcg aagaaatgac gaagctcgga tacacgaatt
      301 cgacggagaa tccagatttc gtgattttga tgcacgttgc tgccgagcag aagttcgacg
      361 agcagaagat ggacgacgtt tataagggct acgacatggc gtggagccag atcagcgacg
      421 acgacgtttg gaacgagggg acgatcatca tcttcgcgat ggacgcaaag acgggacagc
      481 aggtgtggag ctcgacggcg caagcgcggc tccaggacta cgtcggcttc caggaccggc
      541 tggaccgctt caacaaggtg atcaccaaga tgctggccga tttccctccg cgcgcgcagt
      601 aggtcggcca cgaacacggt ccgccgttct ttcgaccggg ctttcttcca gcgctactac
      661 cacgcgccgc gcaccgccgt ggtcggcgcc gacgaagtgc tgaagcgcgc tcgcttcgtc
      721 accagttacc tcgaccatct ccagctcgac gtgcgccgcg tcctcgacgc cgggtgcgga
      781 accggtttgt ggaagcgtgc tctgcagcgc atcgatcgcg acatcgagta cgtcggcatc
      841 gacccgagcg agtacctgtg ccaacgttac gggtggacgc cggcatccat cgacgcgttc
      901 acctcgcggc gcaagttcga cctcgtggtg tgtcaggacg tgctccagta cgcggacgac
      961 gacacggtcg ggcgcagctt cgcgtcgatc gcgcgcgtgt gccgcggagc gctctacttc
     1021 gacgtgccca cccgcgacga catagacgac ggtctgctcg acatgcggcg caccgaccgc
     1081 gcgatccatg ttcgctcggc acggtggtac cgtgcccgtt tggcgaaaga cttcgtgaac
     1141 gtgggggggg gcgtgtttct gcggcgcggc gcgcgcgcgg tggcactcgc actcgagcga
     1201 tgggatagcg cgccgccgcg ggtacggcgg aagcgggact gaacgggcgc gaggggaatc
     1261 gccgcggcta ggcagccggt ggcgtggtca cgtcgcggat gatgtagccg ccggagcgct
     1321 cgtcgggctc gatgtcgatc aaaccctcct gctccatgct cttcaggaga tcgttgaaac
     1381 cgcgaaaacc atagtacgac tcgttgaagc tcggctgccg gcgcttgatg gcctgcttga
     1441 ccatcgaccc ccagatctcg tcttcgtcgc cgcgctcgtc ccaaagcata tcgatggtat
     1501 cgagcaccag gtcgaacgct ttctgccggc gctcctcggt gtccgcctct cccgcggtcg
     1561 cgcgcggcgc ggaggcggcc ttcgctttgg cgggggcgcg cttgcggctc ttgcggcgtt
     1621 tctcgtgctc gcgcaccaaa tcgtcataga agatgaactc gtcacacgcg gtcgtgaaca
     1681 ggtccgacgt cgagttcttg actccgacgc cgatcacgat cttgttgttc tcgcggagct
     1741 tcgtgaccag agacgagaaa tccgagtcgc cgctcatcac cgcgaatgtg ttgacgtggg
     1801 gcttggtgta gcacaggtcg agcgcgtcga ccaccatgcg aatgtcggcc gagttcttgc
     1861 ccgactgccg cacgtgcggg atctcgatca tctcgaaggc cgcctcgtgc atcgggcgct
     1921 tgaagtcctt gtagcggtcc cagtcgcagt acgccttctt gaccacgatg cttcccttga
     1981 ggagcaagcg ttcgaggacg cggctgatgt cgaactcggc gtagcgcgat tcgcgcacgc
     2041 cgatggcgat gttctcgaag tcgcagaaga cggcgatgct ggcggttcgg tctcgttcgt
     2101 tcttttcggg catgaatacc tcgggttggg acggcacgcg gggatcgcga gcgcgggccg
     2161 taggatagca cggcggacgc ggaccggcaa ccgcgcccct tgacgagggc cttgccaggg
     2221 cgtaccgttc gccgattcgc cgccgaatcg gagccgatcg gcggcctccg gaaacgatgg
     2281 aaacaccccg cacgccggac tcgcccccgg acgcaagcga gacgcgatcg gaccccccgg
     2341 tcagtcgtcg cacgcgcctc cgctcgatat tgatcgggcc gccgcgcgac ttgcgtgacc
     2401 ggtcggtcta cgagcacatc tcgctgatcg cgtttctcgc ctgggtcggg ctcggcgccg
     2461 acggactctc gtcgtcatcg tacggccccg aggaggcgtt caaggcgctc ggtgatcata
     2521 cctacctcgc ggtcggcctc gcgctcttga ccgccatcac cgtcttcgtc atttcggcgg
     2581 cgtacaacgg catcatcgaa ttgttcccgc acggcggcgg gggctacttc gtcgccacca
     2641 agctgctcgg caaacgggtg ggcgcggtgt cgggcagcgc gctcgtggtc gactacgtgc
     2701 tgaccatcac tatctccatc gcggccgcgg gcgacgcgct cttcagcttg ttcccgatcg
     2761 agtaccaagt atggaagctg ccggtcgaga tattcctgat tatcgtcctg ttgttgctca
     2821 atctgcgcgg ggtgcgcgag tcggtgctca cgctgacgcc gatctttctg ctgttcatcg
     2881 taacgcacat catcctgatc gtaggcgcga tcttcctgca cgcgtcggag atgcccgcta
     2941 ccgcgcgcga ggtgcaaacc ggcttctcga acgggctcgc gaccctgggg gcgggcgggc
     3001 tggcgctctt gttcattcac gcctactcgc tgggcggcgg cacctacacc ggcatcgagg
     3061 ccgtctcgaa cgggctcgcg atcatgcgcg agccgcgcgc tcagaccggc aagcgcacca
     3121 tgctctacat gtcgatctcg ctcgcgctca cggcggcggg gctgatcctg tgctatctgc
     3181 tctgggacat cgcgcacgtg gaagggaaga cgatgaacgc cgtgctctcc acggcgttcg
     3241 cgggcaactc gaccatgggg caaggattca ccattctcac cctggtgtcg gagggcgcgc
     3301 tcttggtggt ggccgcgcag gcgggattcg tcgggggccc gcgcgtgctc gccaacatgg
     3361 cggtcgactc ctggatgccg caccgcttcg ccgcgttgtc ggaccgcctg gtcaccggca
     3421 acggggtcat tctgatgtgc atcgcctcga tggcggcgct cctctatacg cgcggcgacg
     3481 tgtcgcacat cgtggtcatg tactcgatca acgtgttcgt cactttctcg ctctcgatgc
     3541 tggggatgct gctgtacacg ctgcgcacca agaaggcgtc gcgccgcagc aagcgccgcg
     3601 ttctgctgtt ctcgttcggc ttgctcttgt gcgccaccat cctggttatc acgacactcg
     3661 agaagttcgg cgaaggcggc tgggtcacct tgctcgtgac cggcggcgtg gtggcgctct
     3721 gtttcggcat ccgccgtcac tacgaaagcg tgtcgcgcaa ggtggccaag ctatacgagt
     3781 cgctgatcga ggtcccgcgc gcccccgacg cgcccgacga accgctcgag cccaagcgct
     3841 ccgattcggt agccgcggtc ctggtgggcg actacggcgg cgtgggattg cacacgtgct
     3901 tgagcgtgtt ctccaagttc cccggccact tcaagggcgt ggtgttcgtg tcggtgggtg
     3961 tggtcgactc caaggagttc aagggggaag gcaccgtcga cgcgttgcgt gaccacctcg
     4021 aagaggactt gcagcggtac gtggcgttcg cgcgcaagca aggaatcccg gccacgtatc
     4081 gctactcgat cgacaccgaa gcggtcgagg gcgcggagcg gctgtgtctg gaggtcgcgg
     4141 gcgagttccc gcagatcatg ttcttcgccg gcaaggtgat cttcggcaag gaacagtggt
     4201 tccacccgct cctccacaac gaaaccgcgt acgccattca gcgccgtctg cagtgggccg
     4261 gccagatggt ggtcgtggtg cccattcgag tgatttaggg ccggcgcgat cgtccctcga
     4321 cgcgatttct gcagcgcgaa tgcgacaata ttgtctctct cgcgctgcag tttgagcgga
     4381 ggcgatcgcg ccgacccaag atcctccggg aatcgaagac gacacccttt gccgtgtatg
     4441 ctatcgtgac cggcgccaac cgcgccgata ggaggtcaat cgtgggaaag atcgtagcca
     4501 ttgccgtcgt gatcctgctc gtcttcgtgg cgatcgcagg agtctccggt tacaaccgcg
     4561 tgatcaagct cgaccagggg gtcgcgcagg catggagcca ggtggagaac gtctaccagc
     4621 gccgcgccga cttgatcccc aatctggtcg agaccgtcaa gggcgcggct aacttcgagc
     4681 aggaaacgct gacgcgcgtc atcgaggcgc gcgcggcggc cacgcagatc aagatcacgc
     4741 ccgagatgct caacgacccg cagtcgctgc agcgcttcga gcaggcccag ggagagctct
     4801 cgtcggccct gtcgcgcttg ctcgtcaccg tcgagcagta ccccgagctc aaggccaacg
     4861 cgaattacct ggcgctccag gacgagctcg cgggcaccga gaatcgcatc gccaacgagc
     4921 gacgtcgctt caccgagacc gtgcagacgt acaacaccgc agtcaagagc ttccccatga
     4981 acatgctcgc cggcatgttc ggctttgctc cgaaggctta cttcgaggcc aaggaaggcg
     5041 ccgaggaagc gcccaaggtc gaattctagc gcccgtgcga acgtcccgcg tcactcttcc
     5101 acgccgcgcg cgtctggtcg cgctggcgac gcccgcgctc gtgcttctcg ccgcgcttgc
     5161 ctcggcggcg ccgagcgtac gcgtgcgctt gcccgaagcg ccgtcgcgtt tcgtctacga
     5221 cgaagccggc gcgctctcgt ccgacgagcg gcgcgtcctg gaagacacgc tgatggtcta
     5281 tgaccggcgc ggtctggaga tcggcgttgc gatcttcaag tccctccaag gcgaccccat
     5341 cgaggacgtg tcgatggcgc tcgcggagaa atggaagccc ggcagcgccg agcgcgacaa
     5401 cggcgcgctc atcgtcgtcg gcatggagga gcgcagcgtg cgcatcgaag tgggctacgg
     5461 cctggaagcc gtggtcaacg atgcgatggc cggccgcatc attcgcaacg acatcgcgcc
     5521 cgcgttccgc gagggccgct acggtctggg tctctcgcgc gcggtgtcga gtctcgcgcg
     5581 gctcgcgcgc ggcgagacgc tgccggagcc ggccaacgcc gagctgccgg cgtggctccc
     5641 gctcgtgttc atcctgatct ttattctggt cttcatctcg gtctcgcgcc gcgcccggcg
     5701 cggatggatc tcgcgccagg ggtggaccgg cgggggttgg cggggaccgt acatcggggg
     5761 cggaggcttc ggcggtttcg gagggggcgg ttcgggtggg ggaggcggtt cgttcggtgg
     5821 cggttccttt ggaggcgggg gcgcctccgg gaggtggtga ccgtgaacgg accggcatcc
     5881 tcgcgcgccc tgcgcgccgt cgcggccttc ctggccgcgg ccggggtggt gctcgcgatt
     5941 ctcgtcgccg ccgtggtgat gcgcgagatc gttgcgccgc ccgcgcacac gtggtcggcc
     6001 tggacgcggg gcgccgtctt cgcgatgagc ctggtcgcga tcgtgttctg cgtcctggga
     6061 gggttcctct cctaccgggc gcatcgcgcg cgccgcgact cggtgcgcac gttgctgaca
     6121 agcgaggagc gcgcgcaggt cgcggccgcg attcgagacg cggaagccaa gacgtcggga
     6181 gaggtcgtcg tgcacttggc ggagcgcacc caccacgaac cgaccgtcga ggcgcgcaag
     6241 gcgttcgaga ggatcggcat ggcgcgtacg cgcgaacgca acggcgtgct cttcttcgtc
     6301 tccgtgcgcg accaccggtt cgcggtcatc ggggacaagg gcatccacac gcacgtcgcg
     6361 ccggacttct ggacgggggt tgtcacgcac gtcgagtcgc gtttcgcgca acgccgtttc
     6421 ggcgacgggt tggcggaagg aatcgcgacc gtggccgccg agctcgcgcg atacttcccg
     6481 cgtcgcaccg acgacgtcaa cgagctcccc gatgcgatgt cggacgacgc cggagacgac
     6541 taggttctca ggtgccggtt cgccgcggcg aggaacgcct cgcgcgcggc ggaatgatcg
     6601 acgatgggcc gcggatagtt ctcccccagc acgactccgg cgtggcgcag taggggggcc
     6661 ggcgcctccc acggacggtg gatccacttc gccggaaccc gcgctaattc cgtgacccac
     6721 cggcgcacat acgctccctc gggatcgaat ttccctcctt ggatgatcgg gttgaacacg
     6781 cgaaagtacg cctgcgcgtc gcacccgcac cccgccgacc actgccaccc gaagttgttc
     6841 tgcgcccagt cgccgtcggt caggtacttc atgtaatgcg cctcgccgcg acggtagtcg
     6901 acgcgcaagt gcttggtgag gaagccggcg gcgatcatgc gcgcgcggtt gtgcacgaag
     6961 ccctcgccaa gcagttgccg cgcggcggcg tcgaccacgg ggtagccagt gcgaccctcc
     7021 gtccacgcgc gccagtgcgc ttcgttccgc acccacggga atccttcgaa gccggggcga
     7081 aagggacgca tgagcaagtc ggggcggtcc cacaaggtcg agtgcgcgaa ctcgcgccac
     7141 acgagctcat tcttgaatac gcgcgcggcg tgcgactctc cgagctccga gtctatcgcg
     7201 gtccataccg tccttgccga cagcgtcccg aacttgaggt cttgtgacaa ccggctcgtg
     7261 cccgcacgat ccatgcgatc gcgggcggtg tggtagtgcg cggcgggccc ggaaaggaac
     7321 gcgtccaagc gcgcgcgagc agcgccctcg ccgcccgcga gcaacgcatc gttgcgcacg
     7381 atcccgaccg actccagcgt gggaatgggg atgcgaggag catcgacgcc gggtggaagc
     7441 ggcggcagcg cgcgcggcgc gggcagcggc ttggcgatgg atgccgtcgc gtgaaacgcg
     7501 cgcgcgaacg gcgtgaagac cgagtagggc tcgccggcgg aggttcgcag cgtctcgggc
     7561 ggcaggagca tctcaccttc gtagagaacg aagggcacgc gcagcgcatc ggccacccgc
     7621 cggtcccgct cacgcgcgaa gggctcgacc cagcggtgtg cgaccacgcg gtcgacattc
     7681 cattcggcgg ccagacgcgg aaccacgtcg acgctctttc cctcgaccag gatcaggcgc
     7741 gagcccagag ccgcgatgct cccggatagc tcgcgcagcg actcgagcag gaactggatg
     7801 cggtgcggta tctcctgcgc gcgcgcgggc gcgaaaaaga acgggtcgag cacgaacagg
     7861 gggatgacct cgccgcgcga cgcggcgtcg agcagcggcg ggtggtcgct gacgcggagg
     7921 tccttgcccc gaatccaaac cagcgtgcgc atggccgggc taatctacgc acgccggtgg
     7981 cacccggcaa acccgacctt gccgcaccgc ttcgattggg gcacaatgcc ccgaatgtcg
     8041 ctcgccaacg gcacccggct cggtccctac gaaatcctcg gcacgctcgg cgcgggcggc
     8101 atgggcgaag tctatcgcgc ccgcgacact cggctggacc gcatggttgc gatcaaggtc
     8161 ctgccggcgc atctcgtgag ccagccggaa gtgcgccagc gcttcgagcg cgaggcgcgg
     8221 gcggtttcga gcctcaacca tcccaacatc tgcgtgctcc acgacgtcgg cgccgatttc
     8281 ttcgtcatgg agtacctcga gggagagtcg ctggcgagcc ggatcgagaa ggggccgctt
     8341 ccgaccgccg agctcttgca ggtcggcatc caggtcgcgg acgcactctc cgcggcgcac
     8401 cgcgcggggc tcattcaccg cgacttgaag ccgggcaacg tcatgctaac caagtcgggt
     8461 gccaagctcc tcgacttcgg gctggcacgc tccaccggcc tcgaaggacc caccgacgcc
     8521 actcagtcgc ccaccatgag ccgtccgctg accaaagcgg ggaccatcgt ggggacgttt
     8581 cagtacatgg cacccgagca gctcgaaggc ggcgaggccg acgcgcgcac cgacatcttc
     8641 gccttgggcg ccgtgctcta cgagatggcg acggggcagc gcgccttctc gggcacgagt
     8701 caagcgagcc tgatcgcatc catcttgaag gagcagccgc gcgccatggc ggatctggcg
     8761 ccgatgacgc cgcccgcgct cgagcacctg gtgaagcgtt gcctcgccaa agaccccgac
     8821 gatcgctggc agagcgcgcg cgacgtcggg ctcgagctgg cgtggattcg cgacgccggt
     8881 tccagcgccg gcatttccgc tccggtggcg gcgcaccgtc gaagggccgc gcgcacggcg
     8941 tggatcgcgg cggcggtgtg cgcggccgcg gctatcggct tcgggtccct gctcctcacg
     9001 catcgatcgg cgccgcccat gccgattcgc ttcaccgtcg agccgccggc gaactatggg
     9061 ttcgtaatcg gccagccgca cgtggcggtg tccccggacg ggagcgccct cgcgtactgc
     9121 gccaccgaca ccgttggcgt cgcgcacgtc ttcgtgcgtc ggctcgactc gcacgagtcg
     9181 atccgcatca acggcacgca gggcgcgtcg atgccgttct tctctcccga cggcgcgcac
     9241 ctcgcttact tttcggccgg aaaactgcga cgcgtgtcga tcgccgacgg cgtcgcgcag
     9301 gcgctctgcg acgcgcccga cccccgcggc ggcacgtgga gccgcaacga cgacatcgtg
     9361 tttcagccgg ccgcgtcggg accgatcgtt cgcgtgtcgg cgcgcggcgg cgaaacgcgc
     9421 gtggtcaccg aggtcgacac ggccaacggc gacgccaccc accgcttccc gatgttcctg
     9481 cccgacggaa agcgattcgt ctttgtcgtg ctgccccctc gcgatcagct gttcgacgtt
     9541 cgcgtgggtt cgctcgacgg cgaaaccagc ccggtgatcg cgcgcatggc cggggcaccc
     9601 gtgtattcac gctccgggta tctattgtcg ttccgggacg agcacatcat cgccgccgcg
     9661 ttcgacgcgc gctcgctcga ggtcgaaggc gagcccgtac cgatcggcga gatggccggt
     9721 gtcgggggag gctactccgg cgccgggggc gccaccgtgt ccgaaaacga cgtgctggtg
     9781 cgcacgtttg ccggcacgcc gaacacgacc gtgcagtggc tcggccgcga cggccggccg
     9841 cggggacgca ttacggtacc gtcgggtttc ttcgtcgagc cggtcttctc acccgacggc
     9901 cgccgcatga cgctcgtgga gtggcgtccc gatggtgccg cgaacatctg gatgttcgac
     9961 gtggagcgcg acgtggcgac gcgcttcacg ttcgagaact ccgagaattt cgacgcaatg
    10021 tggtcgccgg atggaaaatc gatcgtgttc acgtcgaacc gggaaggccg cgagaacctc
    10081 tacatcaaac cgagcgatgg gtcgcgtgag gaagagaagc tgttcgactc cggtgagttg
    10141 ttcaccaaga ccgaagcgtg ggcgccggac ggaagcggca tcgtgttcag cgtgctcacc
    10201 gagaagacga acagcgactt gtggttgttc ccgatgacgg gcgatcgcgt gccgcggccg
    10261 ctcctgcaaa cccgattcaa cgagggcgac gcggccattt cgcccgacgg gcgctggatc
    10321 gcctaccgtt ccgacgacgc cggccgggcc gagatctacg tgcgctcgtt cccggatatg
    10381 ggaccgaagc accgcgcctc gtccgaggaa cgcggcgtcc acggcgtctt ccaggcgttc
    10441 tacgcgatcc attgggtcgc gggcggacgc gagctcgtct acctggcggg agacggcagc
    10501 acgctgctgt cggtcggtgt gtcgggtggg gcgacgctga cgctggcgcc gccgcgcccg
    10561 ctgtgccggc tgccgctcgg tcacgccgcg tacgccgccc acccggacgg ccagcgcctg
    10621 atcgtgtgcg ggcccgacgt cacgggcacg ccgtttggcg tcaccgtcgt gatgaattgg
    10681 cccgccctgc tcgggcgcag gtagcccgcc gcgcccgcct tgaggcgtcg ggacggttcc
    10741 tgctataatc gaggtccctt agtaggtccc ggcactcatc ggaagtccaa gcagcctcga
    10801 atgcattacc tattgccgca cctcttgacc cgcgccgctt cccagtcccc cgaccagatc
    10861 gccgtcgtcg ccggatcgga ctcggtgagc taccgggaac tcgatcgcat cagcgatcga
    10921 ttggccgcgg tcctttcttc ccacggcgtt gcccgcggcg atcgtgtggg gatctttctc
    10981 cccaagtcga tgggcgccat cgtcggaatc tatggaatca tgaaagcggg tgccgtctac
    11041 gtgcccgtcg accccgcagc gcccccgggt cgcgtggcct acatcgtcga gaactgcggc
    11101 attcgcgtgt tgctgacgtc ggcgaaaaag gcggcggccg tcgaggagat actctcaaag
    11161 ggtgcggcgc tcgatcgctt gattctactc gacgactccg cgtggagcga cgggccggcg
    11221 gtgccggtgg tgacctggac cgacgtgctc gggcacgacg gaaacgcgcc cgtactcgag
    11281 tccatcgaga ccgatctcgc gtacatcctg tatacatccg gttccacggg cgtccccaag
    11341 ggcgtcatga tctcgcaccg gaactcgctc acgttcgtcg actgggcgca cgacgcggtc
    11401 ggcgcgacga gtgaagaccg cttctcgagc cacgcgccct tgcatttcga cctgtcggta
    11461 ttcgacctgt tcgtcgcgtg caaagccgcc gcgacgctgg ttctcgtccc cgacgggctc
    11521 tcgtcgtttc cgtttcggct cgccgagtgg atcgagaagg agcgcatctc ggtttggtac
    11581 tcggtaccga ccatcctgtc gctcctcgtg cgcagcggcg acctcgggcg cttccgctac
    11641 ccccacctcc gcaccatcat cttcgcgggt gaagtgtttc cggtgaagta cctgcgcgac
    11701 ctcatggccc tggttcccaa ggcgacgtat ttcaatttct acgggcccac cgagaccaat
    11761 gtgatcacgt actatcgcgt gcctcccatt cccgaggacc gcaccgcccc gatcccgatc
    11821 gggatcgcgt gcgccaacac ggaggtgttc gcgatcgccg acgacggcaa gcgagtttcg
    11881 cggacgggcg aggttggcga gctgtacgcg cgcggctcgt tggtcgcaca gggatactgg
    11941 ggagatgccg agaagacgca gcgccacttc gtcgccaacc cattgtcgcg tggctttcgc
    12001 gagaccgcgt atcgcacggg tgacatcgtc acgctggacg acgacggcaa ctacgtgttc
    12061 ctcggacgcc gcgacgacat ggtgaagagc cgtggatatc gcatcgagct gggagaagtg
    12121 gaaacgacgc tgtatcgcca cgaaggcgtt cgggaggccg ccgtggtggc gctgcccgat
    12181 ccgtcgatcg ggaaccggct cgtggcgttc gtcgtgctct cggacgaggc ggccgccgac
    12241 gccgaagatc ttcacgagtt ctgcggcgag cacctgccac ggtacatggt ccccgagagt
    12301 atcgaactgc gcgaccacct gcccaagacg tcgacgggta aggtcgacaa ggtcgagctg
    12361 gcccgccgcg ccgggggtac ggcgccggcg gggaactgat ttcattccgg cctgaaggag
    12421 acgacatgga cgccgaaacg agcatccgcc ggttcatcgt acgcaacctc atgatgaaga
    12481 aggacgagaa cgtcatctcg ctcgacgagc cgcttctgga aaagggcatc gtcgactcgt
    12541 ggggcatcgt cgagttgacg gagtttctca agaaggagtt cggcgccgac ctcgacgacg
    12601 ctgacatcct gcccgagaac ttccacagcg ttcgtactat cgcggccctg gtcaatcgtg
    12661 gaaagtcgta gacgtccggc gcatggactt ttccctcacc caagaacaga ccgagttacg
    12721 cgacgccgtg atccgcttcg cgcgacgcga gctctccgac gacgtcatcc ggcgcgacaa
    12781 ggatggggcg ttctcgcgcg agcactggaa gaagtgcgcg gagttcggca tccagggcat
    12841 gccgttcccg tccgcgtacg gcggccaggg aatggacctc ctcgccacca tattggccct
    12901 ggaggcggtg gggtacgcgt gcaaggacaa cggcctcggc ttcggactgg gtgcgcaaat
    12961 gtggagcgtg cagacgccaa tcaacgagtt cgggaccgag gagcagaagc ggcgctacct
    13021 aaccaagttg tgcagcgggg aatggatcgg cgcgcacgcc atgagcgagc ccgactccgg
    13081 ctccgactcg cacgcgatga acacgctcgc ggtgcgcgac ggcgatcact acgtattgaa
    13141 cggcagcaag accttcgtgt cgagtgcgcc gctcgccgac gtgttcgtca ccttcgcgac
    13201 cctgggcaag gacaagggct tcctcggcat caccggcttc gtcgtcgagc gcgagacgcc
    13261 cggtgtgcgc gtcagcaacg aaatcgagaa gatggggctt cgtaccgatc ccatggcaca
    13321 ggtgtttttc gaagactgcc gcgtgccggt cgggaatcgg ctggggcgcg acgggcaggg
    13381 cgccgcgatc ttccgcagct cgatggaatg ggagcgcggg tgcatcttcg ccccctgcgt
    13441 cggcgcgatg cagcgccagc tcgaagggtg cgtcgagcac gcgcgcacgc gcaatcagtt
    13501 cgggcagccg atcggaaagt tccaggcggt cgcgaaccgt ctggtcgaca tgcggatgcg
    13561 ctgggaagcg tcgcgctggc tgctctatca gatggcgtgg accaagcaac gcgatggcaa
    13621 ggccgcgacc gagtccgcca tcgccaagct ccacatcagc gagtcgtggg tcaagaactg
    13681 cctggacgcg atccaggtcc atggcggcta cggttacact accgagtacg aactcgagcg
    13741 cgacctgcgc gacgcagtgg gcgggacgtt gtactcgggg acgtccgaga tccagcgcaa
    13801 cttgatcgcg cgccagttgg ggctctagcc gtgtgcggca tcctcgcgta ctacaacctt
    13861 cgcgggcagt cgatcgagcg gtcgcaggac gacatcgtcg gcctgcgtga catgatgtgc
    13921 caccgcgggc cggatgcctt cggctacgcg gaggcgcgcg atcgcagttg ggcgctcgcg
    13981 caccggcgcc tttcgatcat cgacctgtcc gagaacgggc gccaaccgat gagtgacacg
    14041 tcgggtcgcg ttcacctctg ttacaacggc gagatctaca actaccgcga attgaaggat
    14101 gggctctcga agcgcggcca caggtttgct tccgagtccg actccgaagt tattctgctt
    14161 ttgtacaagg agatgggagt cgactgcctg ccgctcctgc gcggcatgtt cgccttggtc
    14221 atcgtggacg aggcgcgcgg ttgcatgctg atcgcgcgcg acccggtggg gaagaagccg
    14281 ctctactacg gcgtcgtggg cgacaacgtg atcgtcgcga gcgagccggg cgtcatctca
    14341 cgcgaccgcg ggtatcgcaa ggcggtcgat cccgccgggt tgtattcgct cctcaccatg
    14401 ggagcggtga agtcgc
//
LOCUS       DASPGK010000433         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1393292, whole genome shotgun sequence.
ACCESSION   DASPGK010000433 DASPGK010000000
VERSION     DASPGK010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393292"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /gene="alaS"
                     /locus_tag="VEC56_12120"
     CDS             complement(<1..1830)
                     /gene="alaS"
                     /locus_tag="VEC56_12120"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXV14942.1"
                     /translation="MVSETKNRYAVSRLVQDYLSFFEAREHTRVGSAPLLPEDPTLLF
                     TAAGMVPFKAYYSDPARAPYPRATSCQKCLRAGGKQSDLENVGRTLRHHTFFEMLGNF
                     SFGDYFKKEAIEWAWELSVDVWKLDQARIWVTIFENDDEAFDLWSKHVGFPKERIKRL
                     GKKDNFWGPVGDTGVCGPSSELHYDTGRAGPDGGPGHHDDNDRYIEYWNLVFPQFFLT
                     EAGVYDPLPKPGIDTGMGLERVAFILQDVEDNFHTDEFLPIRRAIAAALPKGSDTKKA
                     ALAVNAASDHVRALTFAIAENIIPSNEGRGYVLRRLLRRALTKMHPFGVREPFLARGV
                     NAVVTSMGGRYPEIAQKVDFIKQVVTGEEARFQDTLEQGMVRLEALFDAAKKSKTVPG
                     ADVFQLYDTFGFPPELTREMAQDRGLEVDMPGFEKAMAEQKTRARKGAKFQRIDIVEQ
                     PIGTSDSTKTASFKRDDIKRTEFDGYDSLDTRAEVAGVADATPSRVARAGGAGDFVEV
                     TTDRTVFYPEGGGQVGDAGRATGAGEVVDTYRAGDLIAHLVKLASGESREALVGRLQS
                     NATVELHVDRERRFATMRNHTATHLLHAALRNVLGTHVAQAGSL"
     gene            complement(1838..2500)
                     /locus_tag="VEC56_12125"
     CDS             complement(1838..2500)
                     /locus_tag="VEC56_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HXV14943.1"
                     /translation="MPETIVERKPRPRDRVWIRLSGGRFFAVPEAAATQFVVGAVLSA
                     EDIERLDRLDQYTRGHDKAMRLLSMRSRSRREIESALRNLGIADAVRRGIIESLEEAG
                     LVDDARFAREFVSVRKDTRRVGPHRLRADMSKLGLHRAVVDKALASYGADEQEALARA
                     LVERSLRGGPVDEKALRRVVALLKRKGYDYSVVNRIAYELARKIPRASDAEATENIPD
                     EW"
     gene            complement(2508..3650)
                     /gene="recA"
                     /locus_tag="VEC56_12130"
     CDS             complement(2508..3650)
                     /gene="recA"
                     /locus_tag="VEC56_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXV14944.1"
                     /translation="MRADEKGKAIELAVQQIEKQFGQGSIMRLGVNHAIVPVEVIPTG
                     SLALDLALGVGGVPRGRIIEVFGPEGSGKTTLTSSIVANAQKLGGMAAFIDAEHAFDV
                     GYAKKIGVDVDNLLISQPDTGEQALEIAEVLIRSGALDVIVVDSVAALVPQAEINGEM
                     GDQHVGLQARLMSQALRKLTGAVSKTKTCLMFTNQIRMKIGVMFGSPETTSGGNALKF
                     YSSVRMDIRRIAAIKEGEAVTGSRTKVKVVKNKVAAPFKTCEFDIIYGEGISYEGDVI
                     DIGSAAGVDIIKKSGTWFAYNDERLGQGKEAARKFLREHPAIANEIAARIRAHFGVDA
                     TKSAPEAGAAKAAEAPAAAMVAETAEGADPLAANPRKKPGVVRARP"
     gene            complement(3811..>4011)
                     /locus_tag="VEC56_12135"
     CDS             complement(3811..>4011)
                     /locus_tag="VEC56_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXV14945.1"
                     /translation="REARDFHAHATVARVKEPLPPSLTDALASVPPLSHPVTRVSSFD
                     LMESRLGRTGASYSVVKRFALP"
BASE COUNT          620 a         1349 c         1296 g          746 t
ORIGIN      
        1 cagcgagccg gcctgtgcca cgtgcgtgcc cagcacgttg cgcagtgccg cgtgcaagag
       61 gtgcgtcgcc gtgtgattgc gcatggtggc gaagcggcgc tcgcggtcga cgtggagctc
      121 gacggtggcg ttgctctgca agcgaccgac cagggcctcg cgcgattcac cgctcgcgag
      181 tttcaccagg tgcgcgatca aatcgcccgc gcgataggtg tcaacgacct cgcccgcacc
      241 tgtcgcccgg ccggcgtcgc ctacttgccc gccaccctcg gggtagaaca cggtgcggtc
      301 ggtggtcact tcgacgaagt cgcccgctcc cccagcgcgc gcgacacggg agggcgtcgc
      361 gtcggcgacg cctgcgactt cggcccgcgt gtcgagcgag tcgtagccgt cgaactcggt
      421 ccgcttgatg tcgtcccgtt tgaacgacgc cgttttcgtc gagtcgctcg tgccgatcgg
      481 ctgctccacg atatcaatgc gctgaaactt cgcacccttg cgggcgcgtg tcttctgctc
      541 cgccatcgcc ttctcgaagc ccggcatgtc cacctcgagg ccgcggtcct gcgccatctc
      601 gcgcgtcagc tcgggcggaa atccaaacgt gtcgtagagc tggaacacgt cggcgccggg
      661 aacggtcttc gatttcttgg cggcgtcgaa cagcgcctcc agtcgaacca tgccctgctc
      721 gagcgtgtcc tggaagcggg cttcctcacc ggtgacgact tgcttgatga agtcgacctt
      781 ctgcgcgatc tccggatacc gtccccccat ggaggtcacg accgcgttga ccccgcgggc
      841 caggaacggc tcgcgcacgc cgaaggggtg catcttggtc agcgcgcgcc gcagcaagcg
      901 tcgcagcacg tagccccgcc cttcgttgga aggaataatg ttctccgcga tcgcgaaagt
      961 cagcgcgcgc acgtggtcgg aggcggcgtt gaccgcgagg gcggccttct tcgtatccga
     1021 tcccttcggg agcgccgcgg cgatcgcgcg ccggatgggc aggaactcgt cggtgtggaa
     1081 gttgtcctcg acgtcctgca ggatgaacgc gacccgctcg agccccatgc cggtgtcgat
     1141 gccgggcttg ggcaacgggt cgtacacgcc ggcttcggtc aggaagaatt gcgggaacac
     1201 gaggttccag tactcgatgt agcggtcgtt gtcgtcgtgg tggccggggc cgccgtcggg
     1261 ccccgcgcgg ccggtgtcgt agtgcagctc ggacgacggc ccgcatactc cggtgtcgcc
     1321 caccggaccc cagaagttgt ccttcttgcc caggcgcttg atgcgctcct tgggaaaacc
     1381 gacgtgcttc gaccacaagt cgaaggcttc gtcgtcgttc tcgaagatgg tcacccaaat
     1441 gcgcgcctgg tccagcttcc aaacgtcaac cgagagctcc cacgcccact cgatcgcttc
     1501 cttcttgaag taatcgccga aggaaaaatt gccgagcatc tcaaagaagg tgtggtggcg
     1561 aagggtgcgt ccgacgttct cgaggtcgct ctgcttgccg cccgcgcgca agcacttctg
     1621 gcagctggtg gcacgcgggt agggagcgcg cgcgggatcg ctgtagtagg ccttgaacgg
     1681 aaccatgccc gcggcggtga aaagcagggt ggggtcctcg ggcaacagcg gcgcgctccc
     1741 gacgcgcgtg tgctcgcgcg cctcgaagaa ggacaggtag tcctgcacga ggcggctcac
     1801 cgcgtagcga ttcttcgtct cgctcaccat gcttgcgtca ccactcgtcg gggatgttct
     1861 cggtcgcctc ggcgtcggac gcgcgcggga tcttgcgcgc gagctcgtac gcgatccggt
     1921 tgaccacgga gtaatcgtac cccttgcgct tcaagagcgc gaccacgcgc cgcagtgcct
     1981 tctcgtcgac ggggccgccg cggagacttc gctccaccag ggcgcgcgcc agcgcctctt
     2041 gctcgtccgc gccgtagctc gcgagcgcct tgtcgacgac ggcgcgatgg agcccgagct
     2101 tgctcatgtc ggcgcgcagg cgatgcggcc ccacgcgccg ggtgtccttg cgcaccgaca
     2161 cgaactcgcg ggcgaagcgg gcgtcgtcga cgagacccgc ttcctcgagc gactcgatta
     2221 ttccgcggcg caccgcgtct gcgattccca ggttacgcaa cgccgactcg atctcgcgcc
     2281 gcgaacgcga tcgcatggac aagaggcgca tggccttgtc gtggccgcgc gtgtactggt
     2341 ccaaccgatc gagtcgttcg atgtcctcgg ccgacagcac cgctccgacc acgaactggg
     2401 tcgcggccgc ttccggaacg gcaaaaaacc gccccccgga gagccggatc catactctgt
     2461 ctcgggggcg cggttttcgc tcgacgatgg tctcgggcat caactagcta cgggcgcgca
     2521 cgcacgacac cgggcttctt gcgcgggttg gccgccaggg ggtcggcccc ttcggcggtc
     2581 tccgcgacca tcgccgccgc gggggcctcg gccgccttgg cggcacccgc ttccggcgcc
     2641 gatttggttg cgtccacgcc gaagtgcgcg cgaatcctgg ccgcgatctc gttcgcgatg
     2701 gccgggtgtt ccctgaggaa cttgcgcgcc gcttccttgc cctgacccag tcgctcgtcg
     2761 ttgtacgcaa accaggttcc gctcttcttg atgatgtcga cgccggcggc cgagccgatg
     2821 tcgatcacgt cgccctcgta gctgatccct tcgccgtaga tgatgtcgaa ctcgcaggtc
     2881 ttgaacggag ccgccacctt gttcttgacc accttcacct tggtccggct gccggtgacg
     2941 gcttcgcctt ccttgatggc cgcgatgcga cgaatgtcca tgcgcaccga cgagtagaac
     3001 ttgagcgcgt tgccgcccga cgtcgtctcg gggctgccga acatgacacc gatcttcatg
     3061 cggatctggt tggtgaacat gaggcacgtc ttggtcttgg agaccgcgcc ggtgagcttg
     3121 cgcagcgcct gcgacatcaa gcgtgcttgc aggcccacgt gctggtcgcc catctcgccg
     3181 ttgatctcgg cttgcggcac cagcgccgcc accgagtcga ccacgatcac gtccaacgcg
     3241 ccgctgcgga tcagtacctc cgcgatctcg agcgcctgct cgcccgtgtc gggttgcgag
     3301 atcaaaagat tgtcgacgtc gacgccgatc ttcttggcgt agccgacgtc gaaggcgtgc
     3361 tcggcgtcga tgaaggcggc catgccgccc aatttctgcg cgttggcgac gatcgacgag
     3421 gtgagcgtgg tcttgcccga gccttcgggt ccgaacacct cgatgatgcg gccgcgcggc
     3481 acgccgccga caccgagtgc gaggtcgagc gccaaactgc cggtcggaat gacttcgacc
     3541 ggaacgatgg cgtggttgac tcccaggcgc atgatcgagc cctggccgaa ctgcttctcg
     3601 atctgctgga ccgcgagctc gatggctttg cctttttcgt cggctctcaa tcccgtagtt
     3661 aatgacttcg ttgacatcgt gtcccccttg gacgttactc gaacggctgg cccttccctt
     3721 ccgcacctct gccccgcgtc cagccgctcg cggggagcct aaactcaagc aatctcatga
     3781 cccacgtgtg agcgaatgct agcacgcccc ctagggcaag gcaaaccttt tgaccaccga
     3841 atacgaggcg ccggtacgcc ctaaacggct ttccatgagg tcgaaactgg aaacgcgggt
     3901 gacggggtgt gacaacggcg gtaccgacgc gagcgcgtcg gtcaaggacg gcggcaacgg
     3961 ctctttgacg cgagctaccg tggcgtgggc atggaagtcg cgcgcttcgc g
//
LOCUS       DASPGK010000434         7495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1042312, whole genome shotgun sequence.
ACCESSION   DASPGK010000434 DASPGK010000000
VERSION     DASPGK010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042312"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1456
                     /locus_tag="VEC56_12140"
     CDS             239..1456
                     /locus_tag="VEC56_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HXV14946.1"
                     /translation="MSSTEANELVHDWNPRPSTPPYALKLNDETLRDGLQSPSVSNPS
                     IPEKLELLHYMEDLGLYSAAIGLPAAGPHAYNDVLAIASEIARHRMKIYPYCAARTHQ
                     NDILPIIDIQQKTGIKVQAALFIFSSPIRQFAEGWTVDKLVDVSEKAIEFAHKNDVPV
                     MYVTEDTTRSRPETLERLYRAAIDRDVERVCISDTVGHATPAGAEAVARFVRSMVTKS
                     GKSIGVDWHGHRDRDVAVANSVAAFYAGADRIHGSMLGIGERVGNTPIDMLMVNFKLM
                     GVFDRDLTKLPRYARRVSEVCRVPIPDNYPVLGRDAFRTQTGVHADAIVKAYKKGDAW
                     LANRIYSGVPAEEFGLAQVIEVGPMSGKSNVRYWLESRSLPADEATVDRIFERAKQVD
                     RVLTDDEIRALVG"
     gene            complement(1517..2182)
                     /locus_tag="VEC56_12145"
     CDS             complement(1517..2182)
                     /locus_tag="VEC56_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14947.1"
                     /translation="MKGTKKGIVLAPFLMVFASLAFAEPSYLIYPNAPAVFRFDASRY
                     ELVLPGDPKFDSNFAVGNQMLWDRVEGRVPVEIYRAPSLLGFEPSTKGQSEFYVVSTD
                     FEIVVDGFGPMQRTIGGLCVRFWPSPAHVQLTIDGSLATGLTHNLDALEVRTAVGGGY
                     YADTSRFMLSWIGAREIRVVAFSDKNGDRAFEGTALYSIVARNETVSTFPTTWGQIKS
                     LYR"
     gene            2551..4218
                     /locus_tag="VEC56_12150"
     CDS             2551..4218
                     /locus_tag="VEC56_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HXV14948.1"
                     /translation="MPRFARWFVVAAWVAVVLAGCGGKDDADNQATSGSPAAQDEQAR
                     SGGSVIRRLESECKTLNWVLYTTAYENFVLRYLYDPVFDYNEAGDMIPVLASQLPDVS
                     PDHLRVTVHLRDDIHWHDGVPITAHDVKFSLDKIQDPGIPALNKEAFFEKLDRLDVVD
                     DRTLVFVWKEPFAPSLFAISQLWPIPKHVYGQGDFLTHASNRKPVGSGPFRFDEWRSG
                     QYISLVRYDDYHGQKAYLDRVIFKVVEDDAVALNMLRTGELDEMRATQPQWERQMSEP
                     EFVARYNKYDYYFPQYNYLAWNCESVWFSDPRIRRAMTMLFDRESINTKIYSGYARLV
                     SGPFYINSWAYDKTIKPYPYDPATAKRLLEEAGWTDSDNDGIRDKNGQKFEFELLHAH
                     GSNTAAQLSQLFQEECGKTGIRVNIRQIEASTFFDRVDKSDFSAAILGWRLDLDPDVF
                     DTFHSAMTPPNGLNHVHYSNPHVDSLLVAGRSEFDQAKRQRIYHQVHRLIHEDQPYTF
                     INTVPDKRPIAKRIHGVKFGWNGPYDFWPGASYWWVDDAPGDAASAR"
     gene            4226..5203
                     /locus_tag="VEC56_12155"
     CDS             4226..5203
                     /locus_tag="VEC56_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14949.1"
                     /translation="MLHYTLRRLLYLVPTLLGITVVTFLIVNLAPGDPVELIHRGQMD
                     ARISPESYQEMLRLYGLDDPVHVRYLTWLKRLATLDFGNSFLDHRPVVDKIMERLPNT
                     VFLNVTSLLIAFLLSIPLGLYAALHQHSKIDKVSGTALYVLYSLPEFWVALLLIMLFG
                     VELRWLPFHGMESLDARKLGFAGYWWDRFLHTILPTVCLTYGSLAYLSRFARGSSLEV
                     VRQDYIRTARAKGLDEASIVYRHVFRNTMIPMVTLLGLFLPTLISGSVIVESIFSWPG
                     VGDLFFTSVRARDYPVVMGLSFITAVMVLLSTLVADLLYAWADPRVTYK"
     gene            5200..6285
                     /locus_tag="VEC56_12160"
     CDS             5200..6285
                     /locus_tag="VEC56_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV14950.1"
                     /translation="MTALAADSLPPAGGIPPATSAPPKSAGFARRVWSRLRRDRYAMA
                     GLVIIGTMFAVSYFAPVIANDKPIVMRWEDRLIFPAVAELFPIRYVVHYPEIAHLDFD
                     IVKYDPAAALVMPPIRYSPIATSLDDRLQKPSRLHWLGTDELGRDVVARMLHGAGISL
                     KVGLVATGISLVIGLLLGALAGYYGGITDILLSRLIEIVTCFPFTFLILAVIAFLPPS
                     IYYIMIVIGITRWTDLARYARGEFMRLKAREFAEAARALGASDAKIIVRHILPNSLAP
                     VLVTATFGVANAILIEAALSFLGLGTQPPTPSWGGILASAREQDFAWWLTTFPGLAIF
                     VTVTAYNLLGEGLRDASDPRVTFAAKR"
     gene            complement(6263..7141)
                     /gene="mutM"
                     /locus_tag="VEC56_12165"
     CDS             complement(6263..7141)
                     /gene="mutM"
                     /locus_tag="VEC56_12165"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXV14951.1"
                     /translation="MPVAPSCILAGMPELPEVETVVRELRAKLPGAVITRAVVTAPDM
                     YRRDSRDVSTLEGARVTGLERLGKAIVIRCESDRGRLDLAVHLGMTGQLLWTARAQEP
                     ALPHLHARWGLSGGAELRLVDPRRFGYVLVGAPETVRASLAIGPDALSITPEELALAL
                     RGRRAAIKALLLDQRIVAGLGNIYVDETLFLARVHPSTGGDRAATRAREILSAARRVL
                     ARAIDARGTTLRDYRRPDGSTGEFQMKLRVYGREGESCPRCRARIRRIEVGQRGTHFC
                     PRCQRAPRRVSASPRR"
     gene            7293..>7495
                     /locus_tag="VEC56_12170"
     CDS             7293..>7495
                     /locus_tag="VEC56_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HXV14952.1"
                     /translation="MSKSMRDILFDEVLPRVSKPGQYAGGERNAVVKDHASVDLKVAL
                     AFHDTYAIGMSHLGLRSLYHLIN"
BASE COUNT         1314 a         2570 c         2320 g         1291 t
ORIGIN      
        1 ccgtctcgag caggactagg aacccgctgc gcgtggactg gaagcggtag acgccgtgcc
       61 gatcgacgag cgccgcgacc accgcgtcgc ggcgagagag gtgacgaacg gccgcgcgca
      121 tctcgtcatc gatgggaggc gcggacgcac cgcgcgggtc gagccaggcc atggcggcat
      181 tatagccgaa ttagttgaag cggcgatgcg cttctgttag cgttagtgcc gatccacgat
      241 gtcctccacc gaagcaaacg aactcgtcca cgactggaac ccgcgcccga gtaccccccc
      301 gtacgcgctc aagctcaacg acgagaccct gcgcgacggc ctccaatcgc cctcggtctc
      361 caacccgtcg atccccgaga agctcgagtt gctccattat atggaggacc tcggactcta
      421 ctccgccgcc atcggcctgc ccgccgcggg accccacgcg tacaacgacg tgctcgcgat
      481 cgcgagcgag atcgcgcgcc accgcatgaa gatctacccc tattgcgcgg cgcgcacgca
      541 ccagaacgac atcctgccca tcatcgacat ccagcagaag accggcatca aggtgcaggc
      601 ggcgctgttc atcttctcga gcccgatccg gcagttcgcc gaaggctgga cggtggacaa
      661 gctggtcgac gtcagcgaga aggcgatcga gttcgcccac aagaacgacg tgcccgtcat
      721 gtacgtcacc gaagacacga cgcgttcgcg gccggaaacg ctggagaggc tgtaccgcgc
      781 cgccatcgac cgcgacgtcg agcgcgtgtg catctcggac acggtcgggc acgccactcc
      841 ggccggcgcg gaggcggtgg cgcgcttcgt gcgttcgatg gtgacgaagt cgggcaagtc
      901 gatcggcgtc gactggcacg gccaccgcga ccgcgacgtc gccgtcgcca actcggtggc
      961 cgcgttctac gccggcgccg accgcatcca cggctcgatg ctcggcatcg gcgagcgcgt
     1021 gggcaacaca ccgatcgaca tgctgatggt caacttcaag ctgatgggcg tattcgaccg
     1081 cgacttgacc aaactgcccc ggtacgcacg gcgcgtctcc gaggtgtgcc gcgtgcccat
     1141 tcccgacaac tatcccgtgc tgggccgcga cgcctttcgc acccagaccg gcgtgcacgc
     1201 ggacgccatc gtcaaggcgt acaagaaggg cgatgcctgg ctcgccaacc gcatctacag
     1261 cggggttccg gccgaggagt tcggcttggc gcaggtgatc gaagtgggac cgatgagcgg
     1321 caaatcgaac gtgcgctact ggctggagtc gcgctcgctc cccgccgacg aggccaccgt
     1381 cgaccgcatt ttcgagcgcg cgaagcaagt cgaccgcgtc ctcaccgacg acgagatccg
     1441 cgccctggtg ggctgaaaaa aaaccgccgc ggcttgcgac cgcggcggtt cccctgcgaa
     1501 cctgcaattc ctgcactcag cgatacagcg acttgatctg cccccacgtc gtcgggaagg
     1561 tagaaaccgt ctcgttgcgc gcgacgatgc tgtagagcgc cgtgccctcg aaggcgcggt
     1621 ctccgttctt gtcggagaag gcgaccacgc gaatctcgcg ggcgccgatc cacgacagca
     1681 tgaagcgcga ggtgtcggcg taatagcccc caccgaccgc ggtcctgact tccagtgcgt
     1741 ccaggttgtg ggtgagaccc gtggccaggc tcccgtcgat cgtcaactgc acgtgcgccg
     1801 ggctcggcca gaaacggacg cacaacccgc cgatcgtcct ttgcatcgga ccgaaaccgt
     1861 cgacgacgat ttcgaaatcg gtgctcacca cgtagaactc gctctgtccc ttggtcgacg
     1921 gttcgaaccc cagcagcgac ggcgcgcggt agatctcgac cggcacgcgt ccttccacgc
     1981 ggtcccacag catttgattg ccaaccgcga agttggagtc gaacttggga tcgcccggca
     2041 gcacgagctc gtagcgactc gcgtcgaacc gaaaaacggc cggcgcattc ggatagatca
     2101 agtacgacgg ctccgcgaac gccaaactcg cgaagaccat aaggaacggg gcgagcacga
     2161 tgcccttctt cgtgcccttc atatgttcga acctccgcgc gagagtcggt gaccggattc
     2221 ttaccggctg accggatccg atgttggtgg agcgctgcga ctcgacgcga ttgatggaat
     2281 agcgcgcggc gttttcaccg cggaatggcc cacttccccc cacaactcgt tgcgggagta
     2341 taacataaac tcatgcgatt tgggaagatg gcgtcgccac aacacgcgta tggcgctccg
     2401 cggtggcttc cttagttcta ttcccacaat aagttagccg cgcacctgcg atcggggttt
     2461 gttgttttcg accggcacgg ccggtgctag actgcgagag gacccgtccc ccacacccag
     2521 gcgggctcga tgttcgaagg aggagtccga atgccgcgtt tcgcccgttg gttcgtcgtc
     2581 gccgcctggg tggccgtcgt gctcgccgga tgcgggggca aggacgacgc cgataaccaa
     2641 gcaacgtccg gctcgcccgc agcccaggac gagcaagcca ggagcggtgg cagcgtcatt
     2701 cgccgcctcg agtccgagtg caagacgctg aactgggttt tgtacacgac cgcttacgaa
     2761 aatttcgttc ttcgctacct ctacgatccc gttttcgact acaacgaggc gggcgacatg
     2821 attccggtgc tcgcgtcgca gcttccggac gtgagcccgg atcacctgcg cgtcacggtg
     2881 cacctacgcg acgatatcca ctggcacgac ggcgttccca tcaccgctca cgacgtcaag
     2941 ttctcgctcg acaagatcca ggatcccggc attcccgcgc tcaacaagga ggcgttcttc
     3001 gagaagctgg accgcctcga cgtcgtcgac gaccgcaccc tggtgttcgt gtggaaggag
     3061 ccgttcgcgc cttcgctgtt cgcgatctcg cagctgtggc ccatcccgaa gcacgtctac
     3121 ggtcaaggcg acttcttgac ccacgcctcc aaccgcaagc cggttgggag cggcccgttc
     3181 cggttcgacg agtggcgctc gggtcagtac atcagcctgg tccgctacga cgactaccac
     3241 ggacagaaag cctacctgga tcgagtcatc ttcaaggtgg tcgaagacga cgcggtggcg
     3301 ctgaacatgc tgcgcaccgg cgaactcgac gagatgcgcg cgacccagcc ccaatgggag
     3361 cgccagatgt ccgagcccga gttcgtcgcg cgttacaaca aatacgacta ctacttcccg
     3421 cagtacaact atctggcgtg gaattgcgag agcgtgtggt tctccgaccc gcgcattcgg
     3481 cgcgccatga cgatgctctt cgatcgcgag agcatcaaca cgaagatcta ctccgggtac
     3541 gcccgcctgg tgagcgggcc gttctacatc aactcgtggg cctacgacaa gaccatcaag
     3601 ccgtacccgt acgaccccgc gaccgccaag cgcctgctcg aggaagccgg gtggaccgac
     3661 tccgacaacg acggcatccg cgacaagaac ggtcagaagt tcgagttcga gctcctgcac
     3721 gcgcacggga gcaacaccgc ggcccagttg tcccagctct tccaggagga atgcgggaag
     3781 acgggcattc gcgtcaacat tcggcagatc gaggcgtcca ccttcttcga ccgcgtcgac
     3841 aaatcggatt tctccgccgc gatcctcggg tggcgtctgg acctcgaccc ggacgttttc
     3901 gacacctttc actccgcgat gacgccgccc aacgggctga accacgtgca ctactccaac
     3961 ccgcacgtgg atagcttgct cgtggccggc cgctccgagt tcgaccaagc gaaacgacag
     4021 cgtatctacc accaggtgca tcgcctcatc cacgaggacc agccgtacac gttcatcaac
     4081 acggttccgg acaagcggcc gatcgcgaag cggatccacg gcgtcaagtt cgggtggaac
     4141 gggccctacg acttctggcc cggcgcgagt tattggtggg tcgacgacgc gcccggcgac
     4201 gccgcttccg cgcggtgacg cgccgatgct gcattacacg ctgcgccggc tgctctacct
     4261 cgttcctacg ctcctcggca tcacggtcgt cacgtttctg atcgtgaacc tggcgcccgg
     4321 tgatccggtc gagttgatcc accggggcca aatggacgcg cgcatctcgc ccgagtccta
     4381 ccaagagatg ctgcgcctct acggactgga cgaccccgtt cacgtgcgct acctcacgtg
     4441 gctcaagcgc ctggcgacac tcgacttcgg caactcgttc ctcgaccacc gtcccgtcgt
     4501 cgacaagatc atggagcgac tgcccaacac cgtctttctg aacgttacgt cgctcctgat
     4561 cgcgttctta ttgtcgatcc cactgggact ctacgccgcc ctccaccagc actcgaagat
     4621 cgacaaagtg agcggcaccg cgctctacgt cctgtactcg ctgcccgagt tctgggtagc
     4681 gctcctcttg atcatgttgt tcggcgtcga gctgcgctgg ctcccgttcc acggcatgga
     4741 atcgctcgac gcgcgcaagc tcgggttcgc gggctactgg tgggaccgtt ttctccacac
     4801 gattctgccc accgtgtgcc tcacgtacgg gtcgctggcc tacctgtcgc gcttcgcgcg
     4861 cggctcgtcg ctcgaagtcg tgcggcagga ctacattcgt accgcgcgcg cgaagggcct
     4921 ggacgaagcg agcatcgtct atcgccacgt gttccgcaac acgatgatcc ccatggtgac
     4981 gctcttgggc ttgttccttc ccacgctgat cagcggcagc gtcatcgtgg agtcgatctt
     5041 ctcgtggccc ggcgtgggcg atctcttctt cacctcggtg cgagcccgcg actaccccgt
     5101 cgtcatgggc ttgagtttca tcaccgcggt catggtgctc ttgagcacac tggtcgcgga
     5161 cctcctgtac gcgtgggccg accctcgagt gacgtacaaa tgaccgccct tgccgccgat
     5221 tcgttgccac ccgcgggagg aataccgccg gccacgtccg cgccacccaa gagcgccggg
     5281 ttcgctcgcc gcgtctggag ccgactccgg cgcgatcggt acgcgatggc gggcctggtc
     5341 atcatcggca ccatgttcgc ggtgtcgtac ttcgctcccg tgatcgccaa cgacaaaccc
     5401 atcgtgatgc gatgggagga ccggctcatc tttcccgcgg tggcggagct gttcccgatc
     5461 cgctacgtcg tgcactatcc cgagatcgcg catctcgact tcgacatcgt caagtacgac
     5521 ccggccgcgg ccttggtgat gccgcccatc cgctactcgc ccatcgctac ctcgctcgac
     5581 gaccgcctgc agaagccgag ccgtctccac tggctcggga ccgacgaatt ggggcgcgac
     5641 gtggtggcgc gcatgctgca cggcgcgggt atctcgctca aggtggggct ggtcgcgacc
     5701 ggcatctcgc tcgtgatcgg gctgttgctc ggcgcgctgg cgggatacta cggcgggatc
     5761 accgacattc tgttgtcgcg cttgatcgag atcgtgacgt gctttccgtt cacgttcctg
     5821 atcctggccg tgatcgcgtt tctgcccccc agtatctact acatcatgat cgtgatcggg
     5881 atcacgcgct ggaccgacct cgcccgctac gcgcgcggcg agttcatgcg gctcaaggcg
     5941 cgcgagttcg cggaagcggc ccgcgccctc ggcgccagcg acgcgaagat catcgtgcga
     6001 cacatcctcc cgaactcgtt ggcgcccgta ctggtgaccg cgaccttcgg cgtcgccaac
     6061 gcgatcctca tcgaggcggc gttgagcttt ttgggattgg gaacgcaacc gcccacgccg
     6121 agctggggcg ggatcctcgc cagcgcgcgc gagcaggact tcgcgtggtg gctgacgacg
     6181 ttccccgggc tcgcgatctt cgtcacggtg accgcgtaca acctgctggg agaggggttg
     6241 cgcgacgcaa gcgacccgcg cgtcaccttc gcggcgaagc gctgacgcgg cgcggtgcgc
     6301 gctggcagcg cggacagaag tgggtccccc gctgcccgac ttcgatgcgg cggatgcgcg
     6361 cgcggcagcg cgggcaagac tctccctccc ggccatacac gcgcagcttc atctgaaact
     6421 cgccggtcga cccgtccggg cgacggtaat cgcgcagggt ggtcccgcgc gcgtcgatcg
     6481 cgcgcgcgag cacgcggcgc gccgcggaga gaatctcgcg ggcgcgcgtc gccgcgcgat
     6541 cgccgccggt cgaagggtgc acgcgcgcca ggaacagcgt ctcgtcgacg tagatgttgc
     6601 cgagcccggc aacgatgcgc tggtcgagga gcagcgcttt gatcgccgcg cgccgcccgc
     6661 gcagtgcgag cgcgagctcc tcgggcgtga tcgagagcgc atccggtccg atcgcgagcg
     6721 acgcgcgcac cgtctccggc gcgcccacca gcacgtagcc gaaccgacgc ggatcgacga
     6781 gccgcagctc cgcgcccccc gagagacccc agcgcgcgtg caggtgcggg agcgccggct
     6841 cctgcgcccg cgcggtccat agcagctgtc ccgtcatccc gagatggacc gccaggtcga
     6901 gccggccgcg atcgctctcg catcgaatca cgatggcctt gcccagccgc tcgaggccgg
     6961 tgacgcgcgc gccctccagg gtcgacacgt cgcggctgtc gcgccggtac atgtcgggag
     7021 cggtcacgac ggcgcgcgtg atgacggcgc cgggcagctt cgcgcgcagc tcgcgcacca
     7081 cggtttcgac ttcgggcagc tcgggcatgc cggcgagtat acacgacggc gcaaccggca
     7141 ttggcgagca tcgagggtcc ccgacgcgaa cttccgcagc gcgataccaa atgtctcgcg
     7201 cgaggacctg agagcggagg ggagccctcg atgcgagcca agccgattga gatggcttgt
     7261 atgcgcgccc gcaacccgct agaatgctcg ccatgtcgaa gtccatgcga gacatcttgt
     7321 tcgacgaagt gctcccccgc gtctccaaac ccggccagta cgcgggtggg gagcgcaacg
     7381 ccgtcgtcaa ggaccacgca tccgtggacc tcaaggtggc gctcgcattc cacgacacct
     7441 acgcgatcgg gatgtcgcac ctcgggcttc gaagtctcta ccacctcatt aatag
//
LOCUS       DASPGK010000435         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1748078, whole genome shotgun sequence.
ACCESSION   DASPGK010000435 DASPGK010000000
VERSION     DASPGK010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748078"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VEC56_12175"
     CDS             <1..555
                     /locus_tag="VEC56_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14953.1"
                     /translation="YVNAVREIIRQTRTGGRRVLGAQDRFFGTAGSDRGKLLTLGLYY
                     LLHNKHTYYMYTSTGDSNTGHISTWGWNPAVQYDIGQPEVNAPGTLDFEGRAGTREHY
                     LFATGADPAVPSLTYRVLARRFTNALVLVKLLPAGSTVDGSSITTHALDGSYGVLQAD
                     GLVGPTVTQASIRNNEALILIPQD"
     gene            complement(552..2372)
                     /locus_tag="VEC56_12180"
     CDS             complement(552..2372)
                     /locus_tag="VEC56_12180"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HXV14954.1"
                     /translation="MLRIATSVPETELTTEKQQLQAKSREVELLAQASRTLNASLEFG
                     ELLGGLLKIVKSAMRAEAVLVSVMDETGSRLVFERALGVDDKEARGSAVTKGAGVMGT
                     VWDKRQPLIINDPKARGEIAFGLEKKLGLKVSALAAIPLMRRGTVRGVLEVVNRKKEE
                     PFGDDDVNLLVPLGEHVAVAVANARLRAEANRRRLEYSLLAEVSADVGKSLTRDEALD
                     RILKNLKKVVDFDAAAIFLVDRKTDSITSVLHSGYPRGATERIHVKMDEGLVGVVAKT
                     KTSIIVPDVRNHPNYHNIRARTRSELVAPMLLRGNVLGLFNLENDRLDAYSENDLRLL
                     EAFASEAAVAIERAQLYEERKVKLEIEEELRIARTVQEFFIPRKSLISGSFRMAGANF
                     PSLEVSGDYYDFFPSMNRLIAFAIADVAGKGVPASLLMASFRAILRTMAPYTATARQI
                     AVRANQILLETVRPGDFVTAFIGVLDPDTGEVSYCNAGHNAPIVMAPDGSYRPLEVGG
                     PVLGILKDAQWLEGRFRLTDEVLVCYTDGATEARNAADEEYGEERFLQSVRANLSLMP
                     YRLCTALYASVRAYTGSSKHTDDTTYLAMRRVDRSAPAAD"
     gene            complement(2473..3564)
                     /locus_tag="VEC56_12185"
     CDS             complement(2473..3564)
                     /locus_tag="VEC56_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXV14955.1"
                     /translation="MTTQDAIRSALEAVIDVYGVGSIVSQNRVERIGVDGDTVMLSLN
                     LPVDDARVKERVEAESREVIKKLGGVREVVVMTKGAKPKLGATAGPQRAGDHAPPGPA
                     RGPSPFESQAPIAGVERIVAVSSAKGGVGKSTVCVNLALALAKRGARVGLMDADVYGP
                     SVHLLMGTNERPQPGNKKEIAPVEKDGLKLMSLGFLTEKGQPVIWRGPIVMGVVKKFL
                     QDVEWGELDYLMIDMPPGTGDAQLTLVQTVPLTGAIIVTTPSELSLVDAEKGLEMYRT
                     VHAPVLGIIENMSYFVCPHCSERTEIFAHGGGRRISERLGVPFLGEIPLDPEVRKVGD
                     EGRPIVQSDPGSPVARAFLEIADRMRAAS"
     gene            3668..4585
                     /locus_tag="VEC56_12190"
     CDS             3668..4585
                     /locus_tag="VEC56_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXV14956.1"
                     /translation="MAADRSYLTRFAWLSIGAAVATIALKAVAYFVTGSVGLLSDALE
                     SFINLVGAVLALAMLTVAARPEDDTHAYGHSKAEYFASGVEGTLIVLAAGAIAFAAVP
                     RLITPRPLEQIGLGLAVSVVAALVNLLVSLVLRRTAKRYDSVTLAASSQHLLADVWTS
                     VGVVAGVGAVGLTGWQRLDPIVALAVATHIAWTGTRIVVESVRGLMDTALPEREQQAV
                     RAALDQYRDSGVHYHALRTRQSGAKRFVSVHVLVPGSWTVQRGHELLEKIESDIRGAL
                     PNVAVLTHLESLDDPASWKDIALERDDTP"
     gene            complement(4589..>5108)
                     /locus_tag="VEC56_12195"
     CDS             complement(4589..>5108)
                     /locus_tag="VEC56_12195"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HXV14957.1"
                     /translation="GRSPMERIQSLVDDLDEEDRELLQNGLFIGRAIDSGKDPLGRGD
                     FLVRGVMGVDSRSGAIAIGDTVRAGERVQFHLRDARTAREDLEMMLSPQALFGAPDGA
                     LLFSCNGRGTRLYDEPDGDISAITSCFRGLDVAGFFCAGEIGPIGGKNFLHGHTASLA
                     LIRTPAPDPKRL"
BASE COUNT          813 a         1739 c         1689 g          867 t
ORIGIN      
        1 tacgtgaacg cggtgcgcga aatcatccgg caaacgcgaa ccggcggccg tcgtgtactg
       61 ggcgcccagg accggttctt cggcaccgcc ggcagcgacc gcggcaagct cttgacgctg
      121 gggctctact acctcctgca caacaagcac acgtactata tgtacacgtc gaccggcgac
      181 tcgaacacgg gacacatctc gacctgggga tggaatccgg ccgtgcagta cgacatcggg
      241 cagcccgaag tcaacgcgcc ggggacgctc gacttcgagg ggcgcgcggg cacgcgcgag
      301 cactacctgt tcgcgaccgg cgccgacccc gcggttccct cgctcaccta tcgcgtgctg
      361 gcgcggcgct tcaccaacgc gctcgtgctg gtgaaactgc tgcccgccgg ctcgaccgtc
      421 gacggcagca gcatcactac ccacgcgctc gacggaagct acggcgtcct gcaagccgac
      481 gggctggtcg gccccaccgt cacgcaggcg agcatccgca acaacgaagc gctgattcta
      541 attccgcagg attaatcggc cgcgggcgcg gaccgatcga cgcgacgcat ggcgaggtag
      601 gtcgtgtcgt cggtatgctt ggaactgccg gtgtaggcgc gcaccgacgc gtacagcgcg
      661 gtgcacagcc ggtacggcat cagcgagaga ttggcccgca ccgattgcag gaatcgctcc
      721 tcgccgtact cctcgtcggc cgcgttgcgc gcctcggtag cgccgtcggt gtagcacacg
      781 aggacttcgt cggtgagtcg aaaacgcccc tccagccact gcgcatcctt gagaatcccg
      841 agcacgggcc cgcccacttc gagcgggcgg taggagccgt ccggcgccat cacgatgggc
      901 gcgttgtgac ccgcgttgca gtagctcacc tcgcccgtat ccggatcgag cacgccgatg
      961 aaggccgtca cgaagtcgcc cggacgtacc gtctccagca ggatctgatt cgcgcgcacc
     1021 gcgatctgcc gcgcggttgc ggtgtagggc gccatcgtgc gcaggatggc gcggaagctg
     1081 gccatcaaga gcgacgccgg cacgcccttg ccggccacgt ccgcgatcgc gaacgcaatc
     1141 aggcgattca tcgaggggaa gaaatcgtag tagtcgccgc tcacttcgag cgacgggaag
     1201 ttcgcccccg ccatgcggaa cgagcccgaa atcagcgact tgcgcggaat gaagaactcc
     1261 tgcacggtgc gcgcgatgcg aagctcttcc tcgatctcga gcttgacctt gcgctcctcg
     1321 tacagctgcg cgcgctcgat ggcgacggcc gcctcggacg cgaacgcttc caggagacgc
     1381 aggtcgttct cggagtacgc gtcgagccgg tcgttctcga ggttgaacag gcccaggacg
     1441 ttgccgcgca ggagcatggg cgcgaccagc tcggagcgcg tgcgcgcgcg gatattgtga
     1501 tagttggggt gattgcgcac gtcgggcacg atgatgctgg tcttggtctt ggccaccacg
     1561 ccgaccagac cttcgtccat cttcacgtga atgcgctcgg tcgcgccgcg cgggtaaccg
     1621 ctgtggagca cgctggtgat ggagtccgtc ttgcgatcga ccaggaagat ggccgcggcg
     1681 tcgaagtcga ccaccttctt gaggttcttg agaatccggt cgagggcttc gtcgcgcgtg
     1741 agcgacttgc ccacgtccgc gctcacctcg gccaagagcg agtactccaa gcggcggcgg
     1801 ttggcctcgg cgcgcaggcg cgcgttggct accgcgaccg ccacgtgctc gcccagcggt
     1861 accagcagat tcacgtcgtc gtcgccgaaa ggctcttctt tcttgcgatt gaccacctcg
     1921 agcacgccgc gcaccgtgcc gcggcgcatg agcgggatgg cggccagcgc ggatactttg
     1981 agacccagct tcttctcgag gccgaatgcg atctcgccgc gcgccttggg atcgttgatg
     2041 atcagcggct ggcgcttgtc ccacaccgtt cccatcaccc cagcgccctt ggtgaccgcg
     2101 ctcccgcggg cttctttgtc gtcgacgccg agcgcgcgct cgaacaccaa tcggcttccc
     2161 gtctcgtcca tgacggatac cagcaccgcc tcggcgcgca tcgccgactt gacgatcttg
     2221 agcagccctc ccaagagctc gccgaactcg agcgaggcgt tgagcgtgcg gctggcctgg
     2281 gccagcagct cgacctcgcg cgacttggcc tgcaattgct gcttttcggt ggtcaattcg
     2341 gtctccggga cgctggttgc gatgcgaagc aagttgtcca tgcttctata actagcgccg
     2401 cggtgtcaag acgatagtaa gagcacgaga agccggaatg aagcctggcc gccgcgcgag
     2461 aaggacacgg cgtcaagacg ccgcgcgcat gcggtccgcg atctcgagga acgctctcgc
     2521 gacgggagag ccgggatcgg attgcacgat ggggcgcccc tcgtcgccca ccttgcgcac
     2581 ctcggggtcg agcgggatct ctcccaggaa cggaacgccc agccgctcgc tgatcctgcg
     2641 gccgccgccg tgcgcgaaga tttcggtacg ctcgctgcag tgcgggcaca cgaagtagct
     2701 catgttctcg atgatgccca ggaccggcgc gtggacggtg cgatacatct cgaggccctt
     2761 ctccgcatcg accagtgaca gctcggacgg agtggtcacg atgatcgcgc ccgtgagcgg
     2821 gacggtttgc accagggtga gctgtgcgtc gccggtcccg ggtggcatat cgatcatgag
     2881 atagtcgagc tctccccatt cgacgtcttg caggaacttc ttcacgaccc ccatcacgat
     2941 gggcccgcgc cagatgacgg gctggccctt ttcggtcagg aacccgagcg acatcaactt
     3001 gagcccgtct ttctcgacgg gagcgatttc tttcttgttc cccggctgcg ggcgttcatt
     3061 ggtgcccatc aggaggtgga cgctgggtcc gtacacgtcg gcgtccatga gcccgacgcg
     3121 cgcgccgcgc ttcgcgagcg cgagcgccaa gttcacgcac accgtcgact tgcccacccc
     3181 gcccttggcc gacgacaccg ccacgatgcg ttcgaccccg gcgatgggcg cttgcgattc
     3241 gaaggggctc ggcccgcgcg ccggacccgg gggcgcgtgg tcgcccgcgc gctgcgggcc
     3301 cgccgtcgct cccagcttgg gcttggcgcc cttggtcatg accacgactt cgcgaacgcc
     3361 acccagcttc ttgatgacct cgcggctctc ggcttcgacg cgctccttga cgcgcgcatc
     3421 gtcgaccggg agattgagcg acagcatcac cgtgtcgccg tcgacgccga ttctctcgac
     3481 gcgattttgc gacacgatgc tgcccacccc gtagacgtcg atgacggctt cgagcgcgga
     3541 gcgaatggcg tcctgtgtag tcatgatgcc accgaactat aacgacccga acgcgggtgc
     3601 gcaaccgcgc atcgttcggg gtcggttgac gccccgttgc gcttcccgta cattcgaccg
     3661 cgcggacatg gcggccgatc gctcatacct gacgcgcttc gcgtggctct cgatcggggc
     3721 cgcggtcgcc acgattgcgc tcaaggcggt cgcgtacttt gtgaccggct cggtcggact
     3781 cctgtccgac gcgctggaat cgtttatcaa tttggtgggc gcggtgctcg cactcgcgat
     3841 gctcaccgtc gccgcgcgcc ccgaagacga cacccacgcc tacggtcact cgaaggccga
     3901 gtacttcgcg agcggcgtgg agggcacgct gatcgtgctg gccgcggggg cgatcgcgtt
     3961 cgcggccgtg ccccgcctga tcacgccgcg gccgctggag cagatcgggc tcgggcttgc
     4021 ggtcagcgtg gtggcggcgc tggtcaacct gttggtctcc ctcgtgcttc gccgaaccgc
     4081 caagcgctac gactcggtca cactcgcggc cagctcgcaa cacctgctcg cggacgtgtg
     4141 gacgtcggtg ggtgtggtgg ccggcgtggg ggcggtcggc ctgaccggct ggcagcgcct
     4201 cgaccccatc gtggccctcg ccgtcgcgac ccacatcgcg tggacgggca cccgcatcgt
     4261 ggtcgaatcg gtgcgcggac tgatggacac ggccctcccg gagcgcgaac agcaggcggt
     4321 gcgcgccgcg ctcgaccagt atcgcgacag cggcgtccac taccatgcgc tgcgcacccg
     4381 tcaatcgggt gccaagcgct tcgtgtccgt gcacgtgctg gtgccggggt cgtggacggt
     4441 gcagcgggga cacgagctgc tggagaagat cgaaagcgac atccgcggcg cactgcccaa
     4501 cgtggcggtg ctgactcacc tggagtcgct ggacgacccc gcttcgtgga aggacatcgc
     4561 gctcgagcgc gacgacacgc cctaggaact acagtcgctt ggggtcgggt gccggcgtcc
     4621 ggatcaaggc caggctcgcg gtgtggccgt gcaggaaatt cttcccgccg atcggcccga
     4681 tctcgcccgc gcagaagaaa ccggccacgt ccaagccgcg gaagcagctc gtgatggcgg
     4741 agatgtcgcc gtcgggttcg tcgtagaggc gcgtgccgcg cccgttgcac gagaacaaga
     4801 gcgcgccgtc gggcgcgccg aagagcgcct gcggcgaaag catcatctcg aggtcctcgc
     4861 gcgcggtgcg cgcgtcgcgc aggtggaact gcacgcgctc gcccgcgcgt accgtgtcgc
     4921 cgatcgcgat cgcgccgctc ctactgtcga cgcccatcac gccgcgcacc aggaagtcgc
     4981 cgcgaccgag cgggtccttg cccgagtcga tcgcgcgccc gatgaacaga ccgttttgca
     5041 ggagctcgcg gtcctcttcg tcgaggtcgt cgaccaggga ctggatgcgc tccatcggcg
     5101 atctcccg
//
LOCUS       DASPGK010000436         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_343519, whole genome shotgun sequence.
ACCESSION   DASPGK010000436 DASPGK010000000
VERSION     DASPGK010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343519"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VEC56_12200"
     CDS             complement(<1..678)
                     /locus_tag="VEC56_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXV14958.1"
                     /translation="MMKAMRENTKIILWIVVVAFVITIFAVWGLDLQSGGMVQQQNTV
                     GRIDGVTITPEAYQAIYSQLSAQFRANNPTGELTGAQQELLREQAWENIVNNVLTGRE
                     VERLGITVTDEEVLNFIRTSPPPEIQQYFQDDKGNFDFAAYQQALNNPEADWTSVEQM
                     VRQRIPVVKLNQYLMSQVQVSQSEITRALQEQHVKLVAEYVAFPIDAETIEGAGPTDE
                     DVAAYYQA"
     gene            888..>2504
                     /locus_tag="VEC56_12205"
     CDS             888..>2504
                     /locus_tag="VEC56_12205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV14959.1"
                     /translation="MYQATRPILAAIAVALAAAAGSSVSAPDEFAWPVALDPGVSSNF
                     CDYRDGRYHAGIDVRTYGREGIPCLAVADGWVSRIRASSRGYGKALHVMLDSGVQVVY
                     AHLAEFVPALEDTLWAEQVRAGRYAVDVTVPKGRFPVRRGDVLAYSGATGATAPHLHF
                     EVRTPEDEPLNPLAHGLRLPDRDRPTIPRIAFVPLSVDATVEGSGFPLELEPRRVAQG
                     RYQIDDTLRFFGDVGVAASVVDRLNGRSGRLAPYELQVWADGTQLSRVTFERFRFDEV
                     KHVDLALDMGALRARGTDVYTLYQRPGDPRLRAEFLRAGRVSPAPSTRRMHEGRLVAW
                     DAAGNRSEVAFHYTDSVVVTVDGRDGRPRLPGERWRQSHLAVDLGGAFFHNDYAVVPV
                     RDPRHFLTQRGHRDEHVVHEDTVLLRARELVGRTRPIARADAADTATVWFSAVPAGEA
                     GRVSFASLGVELEYPADAVYADAVVFARADGDADAGRQRSLRRRSHAVRLGPAGWVLR
                     KNATLRFPVAAPGPRDGVFRSGENGGSWSYL"
BASE COUNT          360 a          854 c          865 g          425 t
ORIGIN      
        1 ggcctggtag tacgcggcca cgtcctcgtc ggtcgggccg gcgccctcga tggtttcggc
       61 gtcgatcggg aaggcgacgt actccgcgac cagcttcacg tgctgttcct gcagcgcacg
      121 cgtgatctcg ctctgcgaaa cctgcacctg cgacatcaag tactggttca gtttgaccac
      181 cgggatgcgc tggcgcacca tctgctcgac cgaggtccag tcggcctcgg ggttgttgag
      241 cgcctgctgg taagcggcga agtcgaaatt gcccttgtcg tcctggaagt attgctggat
      301 ctcgggcggc ggtgaggtgc gaatgaagtt caatacttct tcgtcggtga cggtgatgcc
      361 gagccgctcg acttcgcggc cggtgagcac gttattgacg atgttctccc acgcctgctc
      421 gcgcaagagc tcctgctgcg cgccggtcaa ctcgccggtc gggttgttgg cccggaactg
      481 ggccgagagc tggctgtaga tggcttggta ggcctccggc gtgatcgtga cgccgtcgat
      541 acggcccacg gtgttctgct gttgaaccat gccgcccgac tggaggtcga gaccccagac
      601 ggcgaagatg gtgataacga acgcgaccac cacgatccac aagatgatct tggtgttctc
      661 gcgcatggcc ttcatcatga cgacatcacg actcctggga acgatgggaa caacgcgacc
      721 ggcaacgccg gccgaggggg caagcggcta atctagcaca cggttggcgc gcccgacaag
      781 cacgaccacg gggtgaggga cattcgcgcc cggcacccac tcggttctcg acactcgctc
      841 ggaaccggtg gtagcgtccg acggttccga aaacgaaagt cagatcgatg taccaggcaa
      901 cgagacccat acttgccgcg atcgcggtcg cgctggcggc ggccgcgggt tcgtccgttt
      961 ccgcacccga cgagtttgcg tggccggtgg ccctcgatcc gggggtgtcg tcgaacttct
     1021 gcgactaccg cgacggccgc taccacgccg gcatcgacgt gcggacgtac ggacgcgaag
     1081 ggatcccgtg cctggcggtc gcggacgggt gggtgtcccg catccgcgcg tcgtcgcgcg
     1141 gttacggcaa ggcgctccac gtgatgctcg actccggcgt ccaggtggtg tacgcgcatc
     1201 tcgcggagtt cgttcccgcc ctcgaggaca ccttgtgggc ggagcaagtc cgcgccgggc
     1261 gctacgccgt cgacgtgacc gtgcccaagg gccgttttcc cgtgcgtcgc ggggatgtcc
     1321 tcgcctactc cggcgcgacg ggcgcgaccg cgccccacct gcatttcgaa gtgcggacac
     1381 ccgaagacga gccgctcaat ccgctggccc acggcctgcg gctcccggat cgcgaccgcc
     1441 cgaccatccc gcgcatcgcc ttcgttccgc tctccgtcga tgcgaccgtc gaggggagcg
     1501 gcttcccgct ggagttggag ccccgccgcg tcgcgcaggg ccgttaccag atcgacgaca
     1561 cgttgcggtt cttcggcgac gtcggcgttg cggccagcgt ggtcgaccgg ctcaacggcc
     1621 gctccggccg cctggccccc tacgagctcc aagtgtgggc ggacggaacc cagctctcgc
     1681 gcgtaacctt cgagcgcttt cgcttcgacg aggtgaagca cgtcgatttg gccctcgaca
     1741 tgggcgcgct gcgcgcgcgg ggaaccgacg tctacacgtt gtaccaacga ccgggtgacc
     1801 cgcgcctgcg cgccgagttc ttgcgcgcag ggcgcgtctc gcccgcaccc agcacgcggc
     1861 gcatgcacga gggacggctg gtcgcgtggg acgcggccgg gaatcgctcc gaggtcgcgt
     1921 tccattatac ggactcggtc gtggttaccg tcgacggacg cgacgggcgc ccccgtctcc
     1981 ccggcgagcg ctggcgccag agtcacctcg cggtcgatct ggggggcgcg ttcttccaca
     2041 acgactacgc cgtcgtgccc gtgcgcgacc cgcgccactt cttgacgcaa cggggccacc
     2101 gggacgagca cgtcgtccac gaggacacgg tcctcttgcg cgcgcgcgaa ctcgtcggtc
     2161 gtacgcgacc catcgcgcgc gccgacgccg ccgacacggc gacggtgtgg ttctccgccg
     2221 tccccgcggg cgaagcgggt cgcgtctcgt tcgcgtcgct cggcgtcgag ctcgagtatc
     2281 ccgcggacgc ggtgtacgcg gacgcggtcg tgttcgcacg cgccgacggc gacgcggatg
     2341 ccgggcgcca gcggtcgctg aggcgccggt cgcacgcggt gcgcctgggc ccggcgggct
     2401 gggtgctgcg aaagaacgcc acgctgcggt tcccggtggc cgcgcccggc ccgcgcgacg
     2461 gggtgtttcg ctccggggaa aacgggggat cgtggtcgta cctg
//
LOCUS       DASPGK010000437         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1161248, whole genome shotgun sequence.
ACCESSION   DASPGK010000437 DASPGK010000000
VERSION     DASPGK010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161248"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="nrfD"
                     /locus_tag="VEC56_12210"
     CDS             complement(<1..892)
                     /gene="nrfD"
                     /locus_tag="VEC56_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXV14960.1"
                     /translation="MEHNSAVAAAAAHPASEARLQIERDVIQAMMKPGLLYWGLVGFF
                     ATVFGVCLLGAWAYQIYKGIGVGGQMNPVGWGIYIVNFVFWVGIAHSGTLISAVLFLF
                     RSKFRSSFNRAAEAMTVIAVMCAGMYPLIHLGRVWYAFWLFPYPNQRLLWTNFKSPLE
                     WDVFAISTYLTISVVFFYVGLIPDFAIVKRYTRGLRRHIYSALSLGWVGSKQQWRNYN
                     MLYALLAGFATPLVLSVHSVVSWDFAMGIVPGWHTTIFAPYFVAGAIFSGCGMVLTLC
                     IPMRKIQKLERVMPIDHFEKL"
     gene            complement(882..>3191)
                     /locus_tag="VEC56_12215"
     CDS             complement(882..>3191)
                     /locus_tag="VEC56_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV14961.1"
                     /translation="FSHAAIREANRSLFGVAALPSLRLDRADVVVTVGADVLETFGHP
                     VAFARALSKKHDSHDHFAWFHVEPHASMTGFKASERVSVKPGTEGYFLAYLLHAIAQG
                     RSLDGRASAHLAAVPAVTIDRAAETTGIARETLSKVAEALRNAHAPLLVAGGVSIGHE
                     SGLDVARIAALVQYVAGMHGNTLDFARATDYSRVGSLSDVDRLQARLDAGQVGTAIVF
                     NVDVVSRLSNGDAFGQSLGKASFLVGVGDIMNATLAQCDVVLPLSHAYESWGDVEPVA
                     GVVNVIQPAIKPLFDTLSDGDVLLRILTEMGAVPETTYQQYVMDRWAREHGAAAADLV
                     KTGYIQTSASAPSLSLRDADHRYTFDAVPAKQPTLVIAPSVRWYDGRSAELELLKEIP
                     DPLSTVSWDPWVSVGVETAAALGVDDRDELDIRVGEWSTQLPVRRQPGLQRDVFMIQR
                     GVANPPAGWSRASGEALAVVSDIGVRKTGRKVPTTILSSSLFEDGRGAVPGSKPAHFG
                     ALAKMHEEHAHGGHELKHEDVSFYPVPDYPTYRWAMAIDMDKCVGCSACVAACYIENN
                     VAMSGREQHLRGRELSWIRIEPYYAEDGSSLDTVPTMCQHCDYAPCEPVCPVYATYHN
                     NEGLNVQVYNRCVGTRYCSNNCPYKQRRFNWFSWEHRPYPMDLMTNPDVSMRGKGMME
                     KCTFCVQRIRKARDIAKDEKRDIKEGDFTTACAAACPGGAIVFGNLLDEKSQVHQWAH
                     HERSTRLLEELGTGPAVFYLKKKGSAHGA"
BASE COUNT          565 a         1079 c         1041 g          506 t
ORIGIN      
        1 cgagtttctc gaagtggtcg atgggcatga cgcgctccag cttctggatc ttgcgcatcg
       61 gaatgcacag cgtcagcacc atgccgcagc cggagaagat ggcgccggcc acgaagtagg
      121 gcgcgaaaat ggtggtgtgc caacccggca cgatgcccat cgcgaagtcc caggacacca
      181 ccgagtgcac ggacaacacc agcggggtgg cgaagccggc cagcagcgcg tacagcatgt
      241 tgtagttgcg ccactgctgc ttgctcccca cccatcccag cgagagcgcg ctgtagatgt
      301 ggcggcgaag cccgcgcgtg taccgcttga cgattgcgaa gtcgggaatc aagccgacgt
      361 agaagaacac gacgctgatc gtcaagtacg tgctgatggc aaacacgtcc cactccagcg
      421 gcgacttgaa gttggtccac aacaagcgct ggttggggta cgggaacagc cagaacgcgt
      481 accacacgcg gcccaagtgg atcagcggat acatgcccgc gcacatgacc gcgatcacgg
      541 tcatcgcttc ggccgcgcgg ttgaagctgg agcggaactt ggagcggaac aggaacagca
      601 ccgcgctgat cagcgtgccc gagtgagcga taccgaccca gaacacgaaa ttgacgatgt
      661 aaatgcccca cccgaccggg ttcatctggc cgccgacgcc gatgcccttg tagatctggt
      721 aggcccaggc gccgagcaag cacacgccga acacggtggc gaagaaaccc accagtcccc
      781 agtacaggag cccgggcttc atcatcgcct ggatgacgtc gcgctcgatc tggaggcggg
      841 cctccgacgc cgggtgggcg gccgccgcgg cgaccgcgct gttatgctcc atgtgcgctt
      901 cccttcttct tcagatagaa gacggccggg ccggtgccga gctcttccaa caggcgcgtc
      961 gagcgctcgt ggtgcgccca ctggtgtacc tgcgacttct cgtccaggag attgccgaac
     1021 acgatggcgc cgcccgggca cgccgccgcg cacgcggtgg tgaagtcgcc ctctttgatg
     1081 tcgcgcttct cgtccttggc gatatcgcgg gccttgcgga tgcgctgcac gcagaaggtg
     1141 cacttctcca tcataccctt gccgcgcatc gacacgtccg ggttggtcat gaggtccatc
     1201 gggtacggtc gatgctccca cgagaaccag ttgaagcgtc gctgtttgta cgggcagttg
     1261 ttcgaacagt agcgcgtgcc gacgcaccgg ttatagacct ggacgttgag cccctcgttg
     1321 ttgtgatacg tcgcgtacac cgggcacacc ggctcgcacg gcgcgtagtc gcagtgctgg
     1381 cacatggtgg gcacggtgtc gaggctcgaa ccgtcttccg cataatatgg ctcgatgcga
     1441 atccacgaca gttcgcgccc ccgcaggtgt tgttcccgcc cgctcatcgc gacgttgttc
     1501 tcgatgtagc acgccgctac gcacgccgaa cagcccacgc acttgtccat gtcgatggcc
     1561 atcgcccacc ggtaggtcgg atagtccggc acgggataga acgagacatc ctcgtgcttg
     1621 agctcgtgac cgccgtgcgc gtgctcttcg tgcatcttgg ccagcgcgcc gaagtgcgca
     1681 ggcttgctcc ccggcacggc accccggccg tcctcgaaga gcgacgacga gagaatcgtg
     1741 gtgggcacct tgcggccggt cttgcgaacg ccaatgtcgg acaccaccgc gagggcctcg
     1801 ccgctcgcgc gcgaccagcc cgcgggcgga ttcgccacac cgcgctggat catgaacacg
     1861 tcgcgctgga gcccgggctg gcgccgtacg ggaagctgcg tcgaccactc gccgacgcga
     1921 atgtcgagct cgtcgcggtc gtcgacaccg agcgcggcag ccgtctccac tccgaccgac
     1981 acccacgggt cccacgagac ggtcgagagc gggtcgggaa tttccttcaa caactcgagc
     2041 tcggcgctgc gcccgtcgta ccagcgcacc gacggcgcga tcaccagcgt cggctgcttg
     2101 gcgggcaccg cgtcgaacgt gtagcgatga tcggcgtcgc gcagggaaag cgacggcgcg
     2161 ctcgcagagg tctggatgta gcccgttttc accaggtccg cggcagccgc gccgtgctcc
     2221 cgcgcccagc ggtccattac gtactgctga tacgtcgtct cgggcacggc acccatctcg
     2281 gtgagtatcc gcagcaggac gtcgccgtcc gaaagcgtgt cgaacagcgg tttgatcgcg
     2341 ggctggatca cgttgacgac acccgccacc ggctccacgt cgccccacga ttcgtacgca
     2401 tgcgagagcg gcagcacgac gtcgcactgc gcgagggtgg cgttcatgat gtcgccgacc
     2461 ccgaccagga aggacgcctt gcccagcgac tgcccgaagg cgtcgccgtt ggagagccgc
     2521 gacaccacgt cgacgttgaa cacgatggcc gtgcccacct ggccggcgtc gaggcgcgcc
     2581 tgcaggcgat ccacatcgga cagcgagccc acgcgcgagt agtcggtggc acgcgcgaag
     2641 tcgagggtgt tcccgtgcat gcccgcgacg tactggacga gcgccgcgat gcgcgcgacg
     2701 tccagcccgc tctcgtggcc gatcgacacc ccccccgcga ccaggagcgg cgcgtgcgcg
     2761 ttgcgcaacg cctcggcgac cttggacagc gtttcgcgcg cgatgccggt cgtctcggcg
     2821 gcgcggtcga tcgtcaccgc cggaaccgcg gccaggtgcg cggacgcgcg accgtcgagc
     2881 gagcgcccct gcgcgatcgc gtgcagcagg taggcgagga agtagccctc cgtgcccggc
     2941 ttgaccgaca cgcgctcgct ggccttgaag cccgtcatcg acgcgtgcgg ctcgacgtgg
     3001 aaccacgcga agtggtcgtg cgaatcgtgt ttcttggaaa gtgcgcgcgc gaacgcaacc
     3061 gggtgaccga aggtctccaa tacgtccgcc ccgaccgtca ccacgacgtc ggcgcggtcc
     3121 agccgcagcg acggcagcgc ggccacgccg aacagcgagc ggttggcctc tctaatcgcc
     3181 gcgtgcgaga a
//
LOCUS       DASPGK010000438        17919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_225825, whole genome shotgun sequence.
ACCESSION   DASPGK010000438 DASPGK010000000
VERSION     DASPGK010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 17919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17919
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225825"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VEC56_12220"
     CDS             complement(<1..105)
                     /locus_tag="VEC56_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14962.1"
                     /translation="MTQSIDRLCVDTLRFLAVDAVERAKSGHPGMPMGM"
     gene            459..2177
                     /gene="ggt"
                     /locus_tag="VEC56_12225"
     CDS             459..2177
                     /gene="ggt"
                     /locus_tag="VEC56_12225"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXV14963.1"
                     /translation="MRRFAYVWGLALGLVVVTGSLARAYDRITGRDFASRSEVIAPHG
                     MVATSHPLVTQIALDVLKDGGTAVDAAIAANAALGLMEPTGSGIGGDLFAIVWDAKTK
                     KLYGLNGSGRSPQSLTREMLVSQGLTRIPSHGPLPVTVPGCVDGWIALHERFGKLPLK
                     RVLAPAIAYAKDGFPVPELIAYYWARSAALFGAAPDKYPNFIETYTIDGRAPRKGEIF
                     RNPALARTYEAIAKKGRAGYYEGEVAKTIESFMRERGGHLTAADLAAHHAEWVDPVSV
                     NYRGYDVWELPPNTQGVAALEILNILEGYDFSKIEFGSADHVHYVVEAIKLAFEDAAR
                     YFADPAVAPAPLETLLSKPYAAERRALIDPVRAAKKYEAGGPFGASDTVYLTAADAQG
                     NMISLIQSNYRGMGSGMVPPGLGFMLQDRGELFDLAEGRPNSYAPGKRPFHTIIPGFV
                     TQDGEPFMSFGVMGGSMQPQGHVQVVMNLIDFGMNLQEAGDAPRVRVEGHSESTGERM
                     TDGGTVFLEAGFDTESLRDLAARGHRISFSRDGYGGYQAIRWDRANRVWIGASESRKD
                     GQAAGY"
     gene            2425..3084
                     /locus_tag="VEC56_12230"
     CDS             2425..3084
                     /locus_tag="VEC56_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14964.1"
                     /translation="MVLAASALPAAAGLTVYNCHDHPNGQIIPPPYGLRVDFLLDNGR
                     YTFSFDYVDGSGSAGVTLTHDDVLGTIRIFGRAFGGKDIGGSWDVANRGWIDIDFTYN
                     ANVSVRDNCAGAAGNDLHVIAESALNAGTISLDGWGGNQVFAFTDKADDEGCSFNFDN
                     DWDSKGNSTVANDPSIWSAAGWLKPGDVNGSRDWVFIAEQSTVATEQCSWGHIKAMYR
                     Q"
     gene            complement(3154..3855)
                     /locus_tag="VEC56_12235"
     CDS             complement(3154..3855)
                     /locus_tag="VEC56_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXV14965.1"
                     /translation="MIDFDRIDVISFDCYGTLIDWESGILGALASFRSEYRDRASDDE
                     LLEQYASLESVMESGEYIPYRDVLRGVMRGFARRFSVPEERLDPDLLLNSLPSWRPFP
                     DTVESLRRLKQQYKLAVISNTDDDLFAQTARALEVPFDFVVTAEQVRAYKPSPRNFAH
                     ALEVFGIAKERLLHAAQSRYHDVAPARSLGVATVWVNRRAGKQGEGATALSSAVPDLE
                     VPDLRTLADRAAKSS"
     gene            complement(3859..4350)
                     /locus_tag="VEC56_12240"
     CDS             complement(3859..4350)
                     /locus_tag="VEC56_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14966.1"
                     /translation="MANERMTSAPELSGEFVAASYKQTAIVAGAIATSLVMYAIVAEI
                     ISRSATTGEPPAFLGTLRVALFVVAGAAIFMTTIVKGIMLRNAPADAASRLARLRTAT
                     IASMAVAELPATAGFVLVALGSARADFYMLLAISAYMMVRHFPRRGPWDEYVRRGSTS
                     AVR"
     gene            complement(4340..4768)
                     /locus_tag="VEC56_12245"
     CDS             complement(4340..4768)
                     /locus_tag="VEC56_12245"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXV14967.1"
                     /translation="MRNEWIAVASFSQPVEAHLARTKLESEGIPCLVGDEHLVRVDWF
                     LSNAVGGVKLMVPRSEFERARDALRPRPRLVVVSDRGEPADGEMICPRCRSDDVYYSR
                     YNLRVAGVFILLLGFLIPWRDRRWACTQCGYQWKEASRGK"
     gene            complement(4775..5341)
                     /locus_tag="VEC56_12250"
     CDS             complement(4775..5341)
                     /locus_tag="VEC56_12250"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV14968.1"
                     /translation="MPESQIDVAPYYNQGLSWKQFVDSAGEHASRMRAFYDAFDFDED
                     VLSFFNGRTPLQVLAIAEPWCPDVIQNLAVAARISDEVPGMELSIALRDQSPELMAEY
                     ATGGKHRIPVIAFFDMTFRELARWSGRCKKADAWIFAEVLKGTRDVTSLKGAPAKEFN
                     DEYDRRYREVYVWDTIGEWQHLLEDQEY"
     gene            complement(5351..6235)
                     /locus_tag="VEC56_12255"
     CDS             complement(5351..6235)
                     /locus_tag="VEC56_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV14969.1"
                     /translation="MRRVLWGATSALLLGAGVAVATQTQPDVQVKTIPVAGSVYMLEG
                     QGGNIGACVGPDGILIVDDQYERMADAVRAALTALNPGPLKFILNTHYHGDHVGGNEI
                     FGKEGTIIAHENVRNRVTVPQQRGERTVEPLDPSGWPVVTFLDKVKVHFNGEDIQFIH
                     LPAGHTDGDGIVFFPKSNVIHMGDLLFSGRFPRVDVEGGGSVDGYLKNLDWVSLNLPK
                     DAKVIPGHGPLSTMDDVRAAAGMIRETSEYVKKLIAEKKGLDEIKKAGLPEKFKSFDW
                     QFITTDRWIETLYAAYSK"
     gene            complement(6255..7118)
                     /gene="pgeF"
                     /locus_tag="VEC56_12260"
     CDS             complement(6255..7118)
                     /gene="pgeF"
                     /locus_tag="VEC56_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXV14970.1"
                     /translation="MVGLIERRKDEVRYFAFESLVTHAGVDAVVTTRHGGESTGPFAT
                     MNLGGRTGDDREIVRRNRARAASIVGAAPAWLTFGRQVHGARVAVVGNAERGAVFDDT
                     DALVTNAALVPLVILTADCAAIVLFDPVRRAVGIAHAGWRGTVARVAARTVETMGDAF
                     GSKPEDLVAGIGPSIGPCCYEVGSEVIDAVAAEFPDHADELLVEPEMASAGSFRASVN
                     EEKKHFDLWRANEEVLRAAGVREDTIEVSRVCTSCRTDLFYSHRAEKGNTGRFGGIVM
                     LHEPVLDSRRP"
     gene            complement(7106..8371)
                     /locus_tag="VEC56_12265"
     CDS             complement(7106..8371)
                     /locus_tag="VEC56_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV14971.1"
                     /translation="MRILRASFIVATLALATSGAREPVRDPLDVATAAELEEALSGPR
                     HDAVVRLAPGVYQLTPESAIDSSCGNCADPDTLVPITVGLTISGRNLRIEGPRDGTAT
                     IVTRAGYGLYFKDCADCELERVTLTGGERDTSGLATDAAIVATRSSVTIANCVVRDNI
                     GSADLIAKHTVGIMGVCGREGSDLTIERCEILRNSWDGIALYRAARATISNNLIDGID
                     GAGVEAGGGRGVAIGVTWNGEATIERNLVRKYWKGIGVFVDADVVARGNIVEGMRTWG
                     ISVWDADQGRPRAVIERNVVYDCGACGVAITRKAAYDENEPRGRLVTNIVVHTGQNSK
                     YDAPDYYCYQCALALHAVPEEFSIRGNVFYDNREADGAPFDEDTTREMFWRERRGWVR
                     TFRNTGAGVDERVKFHESAFLTEYPRWWD"
     gene            complement(8424..8966)
                     /locus_tag="VEC56_12270"
     CDS             complement(8424..8966)
                     /locus_tag="VEC56_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14972.1"
                     /translation="MKSDAAAGSGVEKARPVLDARIREGAQRWIPADARASRLSSRAP
                     WWRGFLKPAPMLAGAAAVVVAAVVLWNARGPEEAVLRDDAPVSSPAFSLAPARVEDGS
                     IRLSWTAMPGADAYEVRIYGPDLSEIYRHPATPETSAEVTRSALPADLPSTLDLTWRV
                     YALSGGDVIEASAPGSIRTP"
     gene            complement(9121..9714)
                     /locus_tag="VEC56_12275"
     CDS             complement(9121..9714)
                     /locus_tag="VEC56_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV14973.1"
                     /translation="MPPASKHTWDKTVPDEQLLRIAQGDGDVDRRRAAATELVRRYRD
                     AVYLWCFRYTRDSERALDLSQDVLAAVWEKIGTFEGRSKFSSWLFSVTRNRCIDASRR
                     VDLLADEFELGEVPDPAPLPDAVFEDERDEDWLLRTIRTELEPQEQQAIWLRCVERLS
                     VDDVTRILGVDGASGARAVLQRARRKLRAAIDRRESG"
     gene            complement(9749..12643)
                     /locus_tag="VEC56_12280"
     CDS             complement(9749..12643)
                     /locus_tag="VEC56_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXV14974.1"
                     /translation="MSRTIPAIASLLALLLLTPAPVAQRRDPPRTPADSLAARVEPLF
                     VAAQYDSILAIAALYVRRAEATDDSLLLGRAVAQRGRISLLRGARADAERDIDFCIRV
                     AESVRDTTGLMSAVHFKGFVYRSQGNRDAAMACFQRRLALALAAHAPVDEGWARSSMG
                     LEYHLRGDQSQARDNFTRSIELARASDVRSLEITSLIGLGRVLSAIGEETDAVRCYQR
                     AWVVAREIGDKVNEMWATNNLGVLEETRGDLSRASQYQQRAYELARELKQPYAIVVPA
                     TNLADRAAELGDYETAEAILREMREMCSTQGAETYLPMIDFRLAELQLDQGRSRAAGA
                     SLRRLLAHPERLEPQHRDFAVLDMARILVRSDSTDAAVELISSRLDRADQPAFSDFTI
                     YANMALSSIFLEVGDAGRALAHASAARENAQHAGRRRTVIGAMLRESVCLRALNRGAE
                     AERTFHAALDSIEAFRGGISTAEWREVYGQGVSRGVVDAGRVLLSFPEASAQASRNGA
                     FFDAMQRVKTRALLDRITEPRFGADEIEDRWSRRVATLADVQAVLQPGEALLDFFVGS
                     QESHLAAVTADSLRVVTLPSPDSPLAERVRLFRTLLASEDPDLRGQYPAERMTALQRA
                     LGRDVLGAVGDIIESSTRVFTAPDDYFAAIPFGILITDDGDSPLMARCDIVQVPSASV
                     LVLQRSIRRSECVSNPNLVAIASGEPRLAGARDEVRALARRYGDVEEVTNLAGAESFQ
                     QATQGCDVLHVAAHALVVDRSPWESGIRLASGPAGAIGVPHDEMRAAAASTDILPAAD
                     SLLVARTFQADPYVRAWQIAQLSLPAKLAVLSACETAGGRVTSGEGTLGITAAFLSAG
                     VPVVVSSLWAIDDRVTTDIMRAFYRYLSRGEPVATALRHAQLDLARSRRTAHPFFWAG
                     FTVVGDGSMVVEIEERRGRNPPALLAGSAVMVLAALGWAIRRRRTPASVG"
     gene            complement(12657..13694)
                     /locus_tag="VEC56_12285"
     CDS             complement(12657..13694)
                     /locus_tag="VEC56_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV14975.1"
                     /translation="MATLDRFRLLGRSGLRVSPLCLGTMTFGTEWGWGADKEESRSVF
                     DGYVEAGGNFIDTADYYTGGTSESWLGEFTQGRRDSLVLATKYTMCTTPGDPNAGGSH
                     RKNLVQSLDASLKRLRTDRIDLLWVHAWDATVPIEETMRALDDVVRAGRVLYVGVSNW
                     PAWTTARASALAELRGWTPLVGNQVQYSLVERSIEREILPVSRELGLGVTPWSPLGGG
                     ILSGKYARTGSKAELDQSLRRDSNQRRVNERNLAIVAALEGVAKEVGRSSAQVAIRWL
                     VQKPGVASVILGARTRIQLDDNLQAATFELSPEHMTRLDDAGKIELGYPYDYLRSPTI
                     LKFMYGGAQIE"
     gene            complement(13681..14331)
                     /locus_tag="VEC56_12290"
     CDS             complement(13681..14331)
                     /locus_tag="VEC56_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14976.1"
                     /translation="MDYQSFGAVARLLAVASIFVTVVNAIWRHGDIDLTGRHANMRTI
                     YLRSIVLGVIVAALARINVVAMLRDQAAFDGWLVFPQMDLVDDIVGVLVTGVVIAFLS
                     KLWNDLFDVLYEFKRFLRGKANALRPDEGSSRMRDREGRDGRDGRDRDRDRDMRRGRR
                     GGSRGGRPMGGPGGGDRDNDRFRDRDRDRGDRPERPERPDRDIRRPEIPREPGGNP"
     gene            complement(14519..15823)
                     /locus_tag="VEC56_12295"
     CDS             complement(14519..15823)
                     /locus_tag="VEC56_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV14977.1"
                     /translation="MLTLHSATVTFEGARHPALDRVSLQIEDGSWTAIVGANGSGKST
                     LLGALAGLVSLRSGTLERSGTARVALLQQDADNQLVATSVRHELALSVPVDVAADERA
                     ARIEAAIDRFDLAHPLDRNPHRLSGGEKQRLACATAWLEAPDVLLLDEPLAFLDADAR
                     ARVIDFVREAHARGVTVVWATPDDDAGFAREVVTLDAGRVVNRAPKAAPAVVERPARA
                     ALARGEAVLTIQRASFGYDGRTVLESTDLEVAKGECVGVVGRNSSGKSTLLALAGGAL
                     TPASGRVVRSPATGSVLYLPQTPERMFFAETVREEIAFGVKHLPARRGQASKPVDAII
                     GESLRAVGFDPAEMIARSPFELSFGEMRRVAFAIAHAIAPDLLLLDEPASCLDRAGRD
                     VLAALVASRLEAGAAVVVASHDRTHLDGLCDRVLVLEDGRLS"
     gene            complement(15817..16854)
                     /gene="dusB"
                     /locus_tag="VEC56_12300"
     CDS             complement(15817..16854)
                     /gene="dusB"
                     /locus_tag="VEC56_12300"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXV14978.1"
                     /translation="MVIPRSNGNAGGASVLLAPLCGVTTAPFRRICLEHGADMVVTEM
                     ISSEAMTRGKAEDCKAIRGLDINQGPLSIQIFGGDPVRMGETAARLSELQPVFLDMNF
                     GCPVKKIVNGNGGSAVLKDIDLLGRICREVVRRSSVPVSAKIRAGWDKPTGERVREYA
                     TVIEDAGVSMLSLHARTRSQGFSGVANWDLIAEAKRAVSIPVVGNGDVRSAADVLRMR
                     EHTGCDAVMIGRAAIGNPWVFDEVKTRLAGEDYTQPTPRERVDTMLRHVREAVRIDGE
                     PGGVVAMRKVLAAYLKRLPNARTLRGRLMTVKLLAELEDVMSAYLAEAGPLADVPCGG
                     EVSVEEFASEC"
     gene            16938..17837
                     /locus_tag="VEC56_12305"
     CDS             16938..17837
                     /locus_tag="VEC56_12305"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV14979.1"
                     /translation="MQPTPVIGIPTRDAHPVVADWYAPNGTDHGNVDRVIVMSHGFKG
                     HRRWGFIPIVATRLQFKGFGVLVIDFSHNGHVPADGSVVEEIAEGFVAPELFRANTIA
                     RELDDLTHVLTWLRDPMGGTFLLGLADPAIGLWGHSRGGVISILTALDDPGIAALVTW
                     SALAHPDHYTERQKELWRTKGELAFTDYASGTALALGEAYLDDIEAQRDAYAVGDRAE
                     HLTIPHLIVHGEHDPTVPVADSYKFYDTPTIRADKKLLRLLTGHTYGYDEGRIASDAL
                     DNAIKATEEWFETYLTAPSAQES"
BASE COUNT         2929 a         6069 c         6075 g         2846 t
ORIGIN      
        1 catgcccatc ggcatgccgg ggtggccgga cttggcgcgc tcgacggcgt cgacggcgag
       61 aaaacggagg gtgtcgacgc agaggcggtc gatggactgg gtcaagagag gctcctcggc
      121 tcgaaggggt gcgtggtccg ccgcggatgc ggcggacgag tgcgaaacta ctcccggcca
      181 gcgaggacga tcaagtggat ttgagtgacg gaaagccggg ttgggggggc ggttcgccgc
      241 gctggcgcgc ggtgtctcac ccccgcccgc ggtatttcga acggcgcccc accagaggag
      301 gtcttgggag gtggtcggtt gcccgctcgc gccgctctga aatacgctcg gacggggaaa
      361 tgctgccggt attatacgaa atcgttaacg cttcgtaaac ggcgttcttg gcgtgggggc
      421 gcgggtggac tacactccgg cgcacaggag gaatgctcat gcgccgattc gcctacgtgt
      481 ggggtcttgc tctcgggttg gtcgtcgtca ccggctcgct ggcgcgagcc tacgatcgca
      541 tcacgggccg cgacttcgcg agccgatcgg aagtgattgc gccgcacggc atggtggcga
      601 ccagccatcc gctcgttacc cagatcgcgc tcgacgtgtt gaaagacggc ggcaccgcgg
      661 tcgacgccgc catcgccgcc aacgcggcat tgggattgat ggagccgacg ggctcgggca
      721 tcggcggcga cctcttcgcg atcgtgtggg acgcaaagac caagaagctc tatgggctca
      781 acggcagcgg gcgctcgccg cagtctctga cgcgcgagat gctggtgtcg caagggctca
      841 cgaggatacc gagccacggg ccgctcccgg tgaccgtgcc cgggtgcgtg gacggttgga
      901 tcgcgctcca cgagcgcttc ggcaaactcc cgctcaagcg cgtgctggca cccgcgatcg
      961 cgtacgccaa ggacggcttc cccgtccccg aattgatcgc ctactactgg gcgcgcagcg
     1021 ccgcgctctt cggcgccgcg ccggacaagt atcccaactt catcgagacc tacaccatcg
     1081 acgggcgcgc accgcgcaag ggcgagatct ttcggaatcc cgcgctcgcg cgcacctacg
     1141 aggcgatcgc gaagaagggc cgcgcgggtt actacgaagg cgaggtcgcg aaaaccatcg
     1201 agtcgttcat gcgcgagcgc ggcgggcatc ttaccgccgc cgacctggcc gcgcaccacg
     1261 ccgaatgggt cgacccggtg tcggtcaact atcgcggcta cgacgtgtgg gagctcccgc
     1321 ccaacacgca aggtgtcgcc gcactcgaga tcctgaacat cctcgagggc tacgacttct
     1381 cgaagatcga gttcggcagc gcggatcatg ttcactacgt cgtggaagcg atcaagctcg
     1441 cgttcgaaga cgccgcgcgc tacttcgccg acccggccgt ggcccccgcg ccgctcgaaa
     1501 cgctgctctc gaagccctac gccgcggagc gccgcgcgct catcgatcct gtgcgcgcgg
     1561 ccaagaagta cgaagccggc gggcccttcg gggccagcga caccgtttat ctcacggcgg
     1621 ccgatgcaca gggcaacatg atctcgctca ttcagagcaa ctatcgcggg atgggatcgg
     1681 ggatggtgcc gccgggcttg ggtttcatgc tgcaagaccg cggcgagctg ttcgatctcg
     1741 ccgaaggcag acccaactcg tacgcacccg gcaagcgtcc ttttcacacc atcattcccg
     1801 gcttcgtcac ccaggacggc gagccgttca tgagtttcgg cgtgatgggc ggttcgatgc
     1861 agccccaggg acacgtccag gtcgtcatga acctcatcga cttcgggatg aatctgcagg
     1921 aagcgggcga cgcgccccgt gttcgcgtcg agggtcattc cgagtcgacc ggcgagcgca
     1981 tgaccgacgg cggcacggtg ttcctggaag ccggtttcga caccgagtcg ctccgcgacc
     2041 tggcggcgcg cgggcaccga atctccttta gccgcgacgg ttacggcggc taccaggcca
     2101 ttcgctggga ccgggccaac cgggtctgga tcggggcgtc ggaatcgcgc aaagacgggc
     2161 aggcggcggg atactaggcg ttcccaggcc cgccctgcca ccgcttccgc ggaacgcccc
     2221 gtcgcgacgc catgatcccg ccctgggagg ctggacggcg gaccgcccct cgtgttaatc
     2281 tcccggctat cgcggtgatc tcggtccgca cgggcagccc gtggaacggc ttggcgagcg
     2341 ttcgactaga cccgcttcgc attgaacccg aacctgtgag gtcgatccat gagtcgacgg
     2401 ttaagggctg tgcttctttc ttttatggtg ctcgctgcct cggccctccc ggccgccgcg
     2461 ggactcaccg tctataactg ccacgaccat ccgaacggcc agatcattcc gccgccgtac
     2521 gggttgcggg tcgatttcct tctcgacaac ggccgctaca cgttcagctt cgactatgtg
     2581 gacggcagcg gctcggccgg cgtcacgctg acgcacgacg acgtgctggg caccatccgc
     2641 atcttcgggc gcgcgtttgg cggcaaggac atcggcggga gctgggacgt ggccaaccgc
     2701 gggtggatcg acatcgactt cacctacaac gcgaacgtct cggtgcgcga caactgcgcg
     2761 ggtgcggccg gcaacgacct gcacgtcatc gccgagagcg cgctcaacgc gggcacgatc
     2821 tcgctcgacg gctggggcgg caatcaagtg ttcgccttca ccgacaaggc cgacgacgaa
     2881 ggctgctcgt tcaacttcga caacgactgg gactccaagg gcaactcgac ggtcgccaac
     2941 gacccgtcga tttggtcggc ggccggctgg ctcaagcccg gcgacgtcaa cggctcgcgc
     3001 gactgggtgt tcatcgcgga gcagtcgacg gtcgccaccg agcaatgctc gtggggtcat
     3061 atcaaagcga tgtatcgcca gtaagctcga cgacgacccc ctcgacccaa gcgcccgcgg
     3121 cggttgccgc gggcgtcttt tttgtggaca gttctaagag gacttcgccg cgcgatcggc
     3181 cagggttcgc aggtccggga cttcgaggtc gggcaccgca ctcgacaggg ccgtcgcgcc
     3241 ctcaccctgc ttaccggccc ggcgattgac ccataccgtg gcaaccccga gagagcgcgc
     3301 gggcgcgacg tcgtgatagc ggctctgcgc cgcgtgcagc aggcgttcct tcgcgatccc
     3361 gaacacctcg agcgcgtgtg cgaagttgcg cggggatggt ttgtacgcgc gcacttgctc
     3421 ggcggtgacg acgaaatcga acggcacctc gagcgcgcgc gcggtctggg cgaagaggtc
     3481 gtcgtcggtg ttggagatga cggccagctt gtactgctgc ttcaagcggc gcagcgattc
     3541 caccgtgtcg ggaaacggcc gccagctcgg cagcgagttc aaaagcaggt cggggtcgag
     3601 tcgctcttcg ggcaccgaga agcggcgcgc gaacccccgc atcacgccgc gcagcacgtc
     3661 gcggtacggg atgtactcgc cgctctccat gaccgactcc agcgacgcgt attgctcgag
     3721 cagctcgtcg tcgctggcgc ggtcgcggta ctcggagcga aacgacgcca gcgctcccag
     3781 aatgcccgat tcccagtcga tcagcgtgcc gtagcagtca aaactgatta cgtcgatgcg
     3841 atcgaaatcg atcatggatc agcgaacggc gctcgtgctg ccgcgccgca cgtactcgtc
     3901 ccagggtccg cggcgtggga agtggcgcac catcatgtag gccgagatgg cgaggagcat
     3961 gtagaagtcg gcgcgcgcgc tccccagtgc gacgagcacg aagcccgccg tggcgggcag
     4021 ctcggccacc gccatggagg cgatggtggc ggtgcgcagg cgagccaggc ggctggccgc
     4081 gtcggcgggc gcgttgcgca gcatgatgcc cttgacgatc gtggtcatga agatggcggc
     4141 acccgcgacg acgaacaagg cgacgcgcag cgttcccagg aaagcgggcg gctcgccggt
     4201 cgtcgccgac cgggagatga tctcggccac gatcgcgtac atcaccagcg aggtggcgat
     4261 ggctcccgcg acgatcgcgg tctgcttgta ggacgccgcc acgaattcgc ccgaaagctc
     4321 cggagcgctc gtcatgcgtt catttgccac gggacgcctc cttccattgg tagccgcact
     4381 gcgtgcacgc ccagcggcgg tcgcgccatg gaatcaagaa ccccagcaac aagatgaaca
     4441 cgccggctac gcggaggttg tagcggctgt agtagacgtc gtcgctgcgg cagcgcgggc
     4501 agatcatctc gccgtccgcc ggctcgcctc gatccgacac caccaccagg cgcgggcgcg
     4561 ggcgcagtgc gtcgcgcgct cgctcgaatt cggagcgcgg gaccatcagc ttgaccccgc
     4621 ccaccgcgtt ggacaagaac cagtcgacgc gcacgaggtg ctcgtcgccg accaggcacg
     4681 gaatgccctc ggactcgagc ttggtgcgag cgaggtgcgc ctcgacgggc tggctgaacg
     4741 acgcgactgc gatccactca ttgcgcatgc cgatctagta ctcctgatcc tcaagcaagt
     4801 gctgccactc gccgatcgtg tcccatacgt acacctcgcg atagcgacgg tcgtactcgt
     4861 cgttgaactc tttcgccggc gcgcccttga gcgacgtcac gtcgcgcgtg cccttcaaga
     4921 cctcggcgaa gatccacgcg tccgccttct tgcagcgccc ggaccagcgc gccagctcgc
     4981 gaaaggtcat atcgaagaac gcgatcaccg ggatgcggtg cttgccgccg gtcgcgtact
     5041 ccgccatgag ctcggggctc tggtcgcgca gcgcgatgct caattccatg cccggcactt
     5101 cgtcggaaat gcgcgccgcc acggccaggt tctggatcac gtcggggcac cacggctccg
     5161 cgatcgcgag cacttgcagg ggcgtgcgtc cgttgaagaa cgagagcacg tcctcgtcga
     5221 agtcgaaggc gtcgtagaac gcgcgcatgc gcgacgcgtg ctcgcccgcc gagtcgacga
     5281 actgcttcca cgaaagccct tggttgtagt acggcgcgac gtcgatctgc gactccggca
     5341 tgcgtgactc ctacttggaa tatgcggcgt agagggtttc gatccaacgg tcggtcgtga
     5401 tgaattgcca gtcgaacgac ttgaacttct cgggcagacc ggccttcttg atctcgtcca
     5461 aacccttctt ctccgcgatg agcttcttta cgtactcgct cgtctcacgg atcattccgg
     5521 ccgcggcgcg cacgtcgtcc atggtggaga gcggcccgtg gccggggatc accttggcgt
     5581 ccttggggag gttgagggac acccagtcga ggttcttcag gtagccgtcc accgacccgc
     5641 cgccttcgac gtcgacgcgg ggaaaacgcc ccgagaacag tagatcgccc atgtggatca
     5701 cgttggactt ggggaagaac acgataccgt cgccgtcggt gtgcccggcc gggaggtgga
     5761 tgaactggat gtcttcaccg ttgaagtgga ctttgacctt gtctaggaag gtcaccaccg
     5821 gccagcccga ggggtcgagc ggctcgaccg tgcgctcgcc gcgctgctgc ggaaccgtga
     5881 cgcggttgcg cacgttctcg tgcgcgatga tcgtgccctc cttgccgaag atctcgttcc
     5941 cgcccacgtg atcgccgtgg tagtgcgtgt tcaagatgaa cttcaggggg cccgggttga
     6001 gcgcggtgag cgccgcgcgc accgcgtcgg ccatgcgctc gtattgatcg tcgacgatga
     6061 ggatgccgtc ggggcccacg cacgccccga tgttgccgcc ctggccttcg agcatgtaga
     6121 cggagcccgc caccgggatg gtcttgacct ggacgtcggg ctgggtttgc gtcgcgacgg
     6181 cgacgccggc cccaagcagc agcgcgctcg tcgcgcccca gagtactctg cgcatccgtg
     6241 aacctcgcat ggtgttaggg acgccgcgag tctagcacag gctcgtgaag catcacaatc
     6301 ccgccgaagc gtcccgtgtt tcctttctcc gcgcggtgcg aatagaacag gtcggtgcga
     6361 cacgaggtgc acacgcgcga tacctcgatc gtgtcctcgc gcacgcccgc cgcgcgcaag
     6421 acctcttcgt tcgcgcgcca caaatcgaag tgctttttct cctcgttgac cgaggcgcga
     6481 aagctccccg ccgacgccat ctcgggctcc accagcagct cgtcggcatg gtccgggaac
     6541 tccgcggcga cggcgtcgat cacctcgctt cccacttcgt agcagcaggg cccgatcgag
     6601 gggccgatgc cggccacgag gtcttccggc ttcgacccga acgcgtcgcc catggtctcc
     6661 acggtgcgcg cggccacgcg ggcgacggtg ccgcgccagc cggcgtgtgc gatcccgacc
     6721 gcgcggcgta ccgggtcgaa caacacgatc gccgcgcagt cggcggtgag aatgacgagc
     6781 ggaaccaggg ccgcgttggt caccagcgcg tcggtgtcgt cgaagaccgc gccgcgctcg
     6841 gcgttgccga ccaccgcgac gcgtgcgccg tgcacctggc gcccgaaggt gagccacgcc
     6901 ggcgcggcgc ccacgatgga ggcggcgcgc gcgcggttgc gtcgcacgat ctcgcgatcg
     6961 tcaccggtgc gcccgcccaa gttcatggtc gcgaacggtc ccgtgctctc gccgccgtgg
     7021 cgcgtggtca cgaccgcgtc cacgcccgcg tgcgttacca gcgactcgaa ggcgaaatag
     7081 cgaacctcgt ctttacggcg ctcgatcaat cccaccacct cgggtattcg gtcaggaatg
     7141 cgctttcgtg aaacttgact cgttcgtcga caccggctcc ggtgttccga aacgtccgca
     7201 cccacccgcg gcgctcgcgc cagaacatct cgcgcgtggt gtcttcatcg aacggcgctc
     7261 cgtccgcttc gcggttgtcg tagaacacgt tgccgcggat cgaaaactcc tccggaaccg
     7321 cgtggagcgc aagcgcgcac tggtaacagt agtagtcagg ggcgtcgtac ttggaattct
     7381 ggccggtgtg gaccacgatg ttggtgacga ggcgcccgcg cggctcgttt tcgtcgtagg
     7441 cggccttgcg agtgatcgct accccgcacg cgccgcagtc gtagaccacg ttgcgctcga
     7501 tcacggcgcg cgggcgtcct tggtccgcgt cccacacgct gatcccccac gtgcgcatgc
     7561 cttcgacgat gttgccgcgc gcgacgacgt cggcgtcgac gaagacgccg attcccttcc
     7621 agtactttcg caccagattg cgctcgatgg tggcctcacc gttccaggtc acgccgatcg
     7681 cgaccccgcg cccgccgccc gcttccacgc cggcgccgtc gataccgtcg atcagattgt
     7741 tcgagatggt cgcacgcgcg gcgcgataca gcgcaatacc gtcccacgag ttgcgaagga
     7801 tctcgcagcg ctcgatggta agatccgacc cctcgcggcc gcagaccccc atgatgccga
     7861 ccgtgtgctt cgcaatcagg tccgccgaac cgatgttgtc gcgcacgacg cagttcgcga
     7921 tcgtcaccga gctgcgtgtt gccacgatgg ccgcatccgt cgccagaccc gacgtgtcgc
     7981 gctcgccgcc ggtgagggtg acccgctcca gctcgcagtc ggcgcagtcc ttgaaataga
     8041 gcccatagcc cgcgcgcgtg acgatggtcg ccgtgccgtc gcgcgggcct tcgatgcgca
     8101 ggttgcggcc cgagatcgtg aggcccacgg tgatgggaac gagcgtatcg gggtcggcgc
     8161 agttgccgca ggacgaatcg atcgcactct cgggcgtcag ctggtagaca ccgggggcga
     8221 ggcggaccac cgcgtcgtgg cgcggtccgg agagggcttc ttccaactcg gccgccgtcg
     8281 cgacgtccaa agggtcgcga acgggctcgc gcgcgcccga cgtcgcgagc gcgagggtgg
     8341 cgacaatgaa cgacgcgcgg aggattcgca caccgtcatg ctaaggcagt gcgcgtgcat
     8401 cgccaagcgc cgataccaac gctctacggc gtccgaatcg accccggcgc ggaggcttcg
     8461 atcacgtcgc cgcccgagag cgcgtagacg cgccaggtca ggtcgagcgt ggacgggagg
     8521 tcggcgggca gcgcggagcg cgttacctcc gccgaagtct ccggcgtcgc ggggtgccgg
     8581 tagatctcgc tcaggtcggg gccgtagatg cgaacttcgt aggcgtcggc accgggcatc
     8641 gctgtccacg agagccgaat gctcccgtct tcgacgcgcg ccggcgcgag cgaaaacgcc
     8701 ggcgacgata ccggcgcgtc gtcgcgcaac accgcttctt ccggcccgcg cgcgttccac
     8761 aggaccacgg ccgcgaccac cacggcggcc gcgcccgcca gcatgggcgc gggtttcagg
     8821 aacccgcgcc accacggagc acgcgacgac agtcgcgagg cgcgcgcatc cgccgggatc
     8881 cagcgttggg cgccttcgcg gatgcgggcg tcgagcaccg ggcgcgcctt ctcgacgccc
     8941 gagccggcgg ccgcgtccga cttcatgaac tcaaggtacg actcgaccag actccggcac
     9001 cgcggacacg tctccgcgtg gcgacgatcc ggatgattcg ccggcaagtc ggtgatatcg
     9061 cctattctct caacgtcgat acagtgcgct tccactcgac gttcctccct ccgattggta
     9121 ctacccgctc tcgcggcgat cgatcgccgc gcgcagcttg cgtcgggcgc gctgcaacac
     9181 cgcgcgcgcc cccgacgccc cgtccacccc caggatgcgc gtcacgtcgt cgaccgacag
     9241 tcgttcgacg cagcgcagcc agattgcctg ctgctcttgc ggctccaatt ccgtgcgaat
     9301 cgtccgcagg agccaatcct cgtcgcgctc gtcctcgaac accgcgtccg gcagcggcgc
     9361 cgggtccggc acctcgccca gctcgaactc gtcggccagc agatcgacgc gccggctcgc
     9421 gtcaatgcac cgattccggg tcaccgagaa gagccaggac gagaacttcg accgtccctc
     9481 gaaggtcccg atcttctccc agaccgccgc gaggacgtcc tgcgagaggt cgagcgcccg
     9541 ctccgagtcc cgcgtgtagc ggaagcacca cagatagacc gcgtcgcggt agcgacggac
     9601 cagttccgtg gcggcggcgc gccgccggtc gacgtcgccg tcgccctggg cgatccggag
     9661 cagctgctcg tcggggacgg tcttgtccca cgtatgctta gacgcagggg gcatcgttcg
     9721 tcacaaacga aatgggtcga aggcggaatt atcccacgga cgccggggtg cgtcgacgtc
     9781 ggatcgccca gccgagggcg gcaaggacca tcacggcgga accggcaagg agtgccgggg
     9841 ggttccgccc gcgacgttcc tcgatctcaa ctaccatcga gccgtcaccg acgacggtga
     9901 aaccggccca gaaaaacggg tgcgcggtcc ggcgcgagcg ggcgagatcg agctgggcgt
     9961 gtcgcagcgc ggtcgccacc ggctccccgc gggagaggta ccggtagaac gcgcgcatga
    10021 tgtcggtggt gacgcggtcg tcgatcgccc acaagctcga tacgaccact ggaacgcccg
    10081 cgctcaagaa cgccgccgtg atgccgaggg tgccttcgcc gctggtgacg cggccgcccg
    10141 cggtctcgca cgcggagagc acggcgagtt tggccgggag cgaaagctgc gcgatctgcc
    10201 aggcgcgcac gtagggatcg gcctggaagg tccgggccac caagagcgaa tcggcggcgg
    10261 gcaggatatc ggtcgacgcg gcggcggcgc gcatctcgtc gtgcggcacg ccgatcgccc
    10321 ccgcgggtcc gcttgcgagc cggatgccgg actcccacgg cgagcggtcg accaccagcg
    10381 cgtgggccgc gacgtgcagc acgtcgcacc cttgggtcgc ctgctgaaag gattccgcgc
    10441 cggcaaggtt ggtgacctct tcgacgtcgc cgtaccggcg cgccagggcc cggacttcgt
    10501 cgcgcgcgcc cgccaggcgc ggctcgcccg acgcgatggc aaccaaattg ggattgctca
    10561 cgcactcgga gcgccgaatc gaacgctgga gtacgagcac gctcgcggaa ggaacctgga
    10621 cgatgtcgca tcgggccatg agcggcgagt cgccgtcgtc ggtgatgaga atcccgaacg
    10681 ggatggcggc aaagtaatcg tcgggtgcgg tgaatacccg tgtggacgac tcgatgatgt
    10741 cgcccaccgc gcccaggacg tcgcgaccga gcgcgcgctg cagggccgtc attcgctcgg
    10801 cgggatactg gccgcgcaga tccgggtctt cgctggccag cagagttcga aacaagcgaa
    10861 cccgctcggc caggggtgaa tcgggactcg gtagcgtcac cacgcgcagc gagtccgccg
    10921 tgaccgcggc gagatgcgac tcctgcgacc ccacgaagaa atcgagcagc gcctcgcccg
    10981 gctggagaac ggcctgcacg tcggcgagcg tggcgacccg gcgggaccag cgatcctcga
    11041 tctcgtcggc cccgaagcgc ggctcggtga tgcgatcgag cagcgcccgc gtcttgacgc
    11101 gctgcatcgc gtcgaaaaac gccccgttgc gcgaggcctg cgcggacgcc tccggaaacg
    11161 acagaagcac gcgtccggca tcgacgacgc cgcgcgacac cccctgcccg tacacctcgc
    11221 gccactccgc cgtgctgatg ccgccgcgaa acgcctcgat actgtcgagc gcggcgtgga
    11281 aggtccgctc ggcttcggct ccgcggttca gagcgcgaag acacacgctc tcgcgcagca
    11341 tcgcgccgat caccgtgcgg cggcgcccgg cgtgttgcgc attctcgcgc gccgccgacg
    11401 cgtgagccag cgcgcggccg gcgtcgccta cttcgaggaa gatcgacgat aacgccatgt
    11461 tggcgtagat cgtgaaatcg ctgaacgcgg gctggtcggc gcggtcgagg cgcgacgaga
    11521 tgagttcgac ggccgcatcc gtgctatcgc tccggaccag gatgcgcgcc atatcgagca
    11581 cggcaaagtc tcggtgctgg ggctcgaggc gttcggggtg cgcgagcagc cggcgcagcg
    11641 acgcaccggc ggcgcgggac ctgccctggt cgagctggag ctccgcgagg cggaaatcga
    11701 tcatcggcag gtaggtctcg gcaccctgcg tgctgcacat ctcgcgcatc tcgcgcagga
    11761 tcgcctcggc ggtctcgtaa tcgccgagct cggcagcgcg atcggcgaga ttggtggcgg
    11821 gaacgacgat cgcgtacggc tgcttgagct cgcgcgccag ctcgtacgcg cgctgctggt
    11881 actgcgacgc gcggctcaag tcgccgcggg tctcctcgag taccccgagg ttgttagtcg
    11941 cccacatctc gttgaccttg tccccgatct cgcgcgccac cacccacgcg cgctggtaac
    12001 agcgcacggc gtcggtctct tcgccgatcg cactcaacac gcgacccaat ccgatcaggg
    12061 acgtgatctc gaggctgcgc acatccgacg cgcgcgcgag ctcgatcgag cgcgtgaagt
    12121 tgtcgcgcgc ttggctctgg tcgcccctga ggtggtactc gaggcccatg ctcgagcgcg
    12181 cccagccctc gtcgacgggt gcgtgcgcgg ccagggcgag ggcgagccgg cgctggaagc
    12241 acgccatggc cgcgtcgcgg ttgccctggc ttcgatagac gaaccccttg aagtgcaccg
    12301 cggacatgag cccggtggtg tcgcggaccg attcggcgac tcggatgcag aagtcgatgt
    12361 cgcgttcggc gtcggcgcgc gcgcctcgca ggagcgatat gcggccgcgc tgcgcgacgg
    12421 cgcggcccaa gaggagcgag tcgtcggtcg cctcggcgcg gcgaacgtag agggcggcga
    12481 tggcgaggat cgaatcgtac tgcgcggcga cgaacagggg ttcgacgcgg gccgccaacg
    12541 agtcggccgg cgtgcgcggg gggtcgcgac gctgggcgac gggggccggc gttaggagaa
    12601 gcagcgcgag gagcgacgcg atggccggga tggttcggga catggcggga acgcggttac
    12661 tcgatctgcg cgcctccgta catgaacttg aggatggtcg gactgcgcag gtagtcgtag
    12721 ggatagccga gctcgatctt gccggcgtcg tcgaggcgcg tcatgtgctc gggcgatagt
    12781 tcgaaggtgg ccgcttgcag gttgtcgtcg agttgaatcc gcgtgcgcgc gcccaggatg
    12841 acgctggcca cacccggctt ctgcacgagc cagcgaattg caacttgcgc gctcgagcgc
    12901 ccgacttcct tcgccacgcc ttcgagcgcg gcgacgatgg caaggttgcg ctcgttgacg
    12961 cgccgttggt tggaatcgcg gcgcaggctc tggtccagtt cggccttgct gcccgtgcgc
    13021 gcgtacttgc ccgagagaat cccaccgccc aggggcgacc acggggtgac acccaggccc
    13081 agttcccggc tcacgggtag aatttcgcgc tcgatggatc gctcgacgag cgaatactgc
    13141 acctggttgc cgaccaacgg cgtccacccg cgcagctcgg cgagcgcgct ggcgcgcgcg
    13201 gtcgtccacg cgggccaatt cgacacgccc acgtagagca cccggcccgc gcgcacgacg
    13261 tcgtcgagcg cgcgcatggt ctcttcgatg gggacggtgg cgtcccacgc gtgcacccac
    13321 aacaggtcga tgcggtcggt gcgcaggcgc ttcaagctcg cgtcgagcga ttggaccagg
    13381 ttcttgcggt gcgagccccc ggcgttgggg tcgccggggg tggtgcacat cgtgtacttg
    13441 gtggccaata ccaacgaatc gcgacgaccc tgcgtgaact cgccgagcca cgattcgctg
    13501 gtgccgccgg tgtagtaatc ggcggtgtcg atgaagttgc cgcccgcttc gacgtagccg
    13561 tcgaagacgc tgcgcgactc ttccttgtcg gccccccaac cccactcggt cccgaacgtc
    13621 atcgtgccga ggcacaacgg cgatacgcga agcccgcttc ttcccagcag gcgaaagcga
    13681 tcaagggttg ccaccgggct ccctcggtat ctcgggacgc cgaatatcgc gatcggggcg
    13741 ctccggacgc tcgggacgat cgccgcggtc gcggtcccga tcgcggaagc gatcgttgtc
    13801 gcggtcaccg cccccgggcc cgcccatggg acgcccgccg cgcgagccgc cgcgacgacc
    13861 ccggcgcatg tcgcggtcgc gatcgcgatc gcggccgtct cggccgtcgc gaccttcgcg
    13921 gtctcgcatg cgcgaggatc cttcgtcggg ccggagcgca ttggccttcc cgcgcaggaa
    13981 gcgcttgaac tcgtagagca cgtcgaagag gtcgttccag agcttggaca ggaatgcaat
    14041 gacgacgccg gtcacgagca cgccgacgat gtcgtcgacg aggtccatct ggggaaatac
    14101 cagccagccg tcgaacgccg cttggtcgcg gagcatcgcg accacgttga tccgcgcgag
    14161 tgcggcgacg atgacgccca gcacgatcga gcgcagatag atggtccgca tgttggcgtg
    14221 acgcccggtc aggtcgatgt cgccgtggcg ccagatcgcg ttgacgacgg tcacaaaaat
    14281 gctcgccacg gcgagcaggc gcgcgacggc gccaaatgat tgataatcca cgtgttccct
    14341 cctcctgagg taaggctgga tagtaccggt cggcggcccc gcgcgtcaac gggagcctaa
    14401 accggcggac gctgcccggc gggctccgag cgtcccgcgg cgggtggcgc cggtggggcc
    14461 cccgccgcgc caggcggcgg ggtgaccggt gacaggaccc gccgcgcagc gtgggagctt
    14521 agctgagtcg cccgtcctcc aggacgagga cgcggtcgca gaggccgtcg aggtgggtgc
    14581 gatcgtggga ggcgacgacg acggcggcgc cggcctcgag gcgcgaggcg accagggcgg
    14641 cgagcacgtc gcggcccgcg cggtcgaggc acgatgcggg ctcgtcgagc aggagcagat
    14701 cgggggcgat ggcgtgcgcg atcgcgaagg cgacgcggcg catctcgccg aagctcagct
    14761 cgaagggcga gcgcgctatc atctccgcgg ggtcgaagcc taccgcgcgc agactctcgc
    14821 cgatgatcgc gtcgacgggt ttcgaggcct ggccgcggcg cgcgggcagg tgcttgacgc
    14881 cgaacgcgat ctcctcgcgc accgtctcgg cgaaaaacat gcgctccgga gtctgcggca
    14941 ggtacaacac gctgccggtg gcgggcgatc gcaccaccct accggatgcc ggtgtcagcg
    15001 ccccgcccgc caatgcgagc agcgtcgact tgcccgaact gttgcggccg acgacgccga
    15061 cgcactcccc ctttgcgact tcgaggtccg tcgactcgag cacggtgcgg ccgtcgtacc
    15121 cgaacgaagc gcgctggatc gtgagcacgg cctcgccgcg cgcaagcgcg gcgcgggccg
    15181 gcctctctac gacggccggc gcagcctttg gcgctcgatt gacaacacgt cccgcgtcca
    15241 aggtcaccac ctcgcgcgcg aatccggcgt cgtcatccgg cgtcgcccac accaccgtaa
    15301 ccccgcgtgc gtgggcctca cgcacgaagt cgatcacgcg cgcgcgcgcg tcggcgtcca
    15361 ggaaggcgag gggttcgtcc aagagaagaa cgtcgggcgc ttcgagccag gcggtcgcgc
    15421 acgcgaggcg ctgcttttcg ccgccggaga gccggtgggg attgcgatcg agcgggtgtg
    15481 ccaagtcgaa gcggtcgatg gcggcttcga tgcgcgcggc gcgttcatcg gcggcgacgt
    15541 cgacgggaac cgagagggct aactcgtggc gaacgctggt cgcgacgagc tggttgtcgg
    15601 cgtcttgctg gaggagtgcg acgcgcgccg taccactgcg ctcgagcgtg ccggagcgca
    15661 gcgacaccaa gcccgcgagc gcgcccagca aggtggactt gccggagccg ttggcgccga
    15721 cgatggccgt ccacgatccg tcttcgattt gtagtgagac gcggtccagc gcggggtgtc
    15781 gcgcgccttc gaaagtgacg gtggcactat ggagggtcag cactcggagg cgaactcttc
    15841 gacgctcacc tcgccgccgc agggcacgtc ggcgagcgga cccgcttcgg ccaggtaggc
    15901 gctcatcacg tcttccaatt cggcgagcag cttgaccgtc atcaggcggc cgcgcagggt
    15961 acgcgcgttg ggaagccgct tcaagtacgc cgccaagacc tttcgcatcg cgaccacacc
    16021 gcccggctcg ccgtcgatgc ggaccgcctc gcgcacgtgg cgcagcatgg tgtcgacgcg
    16081 ctcgcgcggt gtcggctgcg tgtagtcttc gcctgcgagc cgcgtcttca cttcgtcgaa
    16141 gacccacggg ttgccgatgg cggcgcggcc gatcatgacg gcgtcgcagc ccgtgtgctc
    16201 gcgcatacgc aacacgtcgg cggcgctgcg cacgtcgccg ttgccgacca ccgggatcga
    16261 cacggcgcgc ttggcctccg cgatcaagtc ccagttggcg acccccgaga agccttgcga
    16321 gcgggtgcgc gcgtggagcg agagcatcga cacgccggcg tcctcgatca cggtcgcgta
    16381 ctcgcgcacg cgttcgcccg tgggtttgtc ccagcccgcg cgaatcttgg ccgacaccgg
    16441 taccgagctg cgacgcacca cttcacgaca aatgcggccg agcagatcga tatccttgag
    16501 gaccgccgag ccgccgttgc cgttgacgat ctttttcacc gggcagccga agttcatgtc
    16561 gaggaagacg ggctgcagtt cggagagccg cgcggccgtc tcgcccatgc gcacggggtc
    16621 gccgccgaag atttggatgg agagcgggcc ttggttgatg tcgagcccgc ggatggcctt
    16681 acagtcttcc gctttgccgc gcgtcatcgc ttcggagctg atcatctcgg tgaccaccat
    16741 gtcggcgccg tgctccaaac aaatacgccg aaacggcgcc gtggtgacac cgcacaaggg
    16801 ggcgagcagg acggacgcgc cgcccgcgtt tccattcgaa cgagggatga ccataggatg
    16861 ggctccataa tagtcgcgcg gcttgcattg cgcaaccgaa ttgcctatcg tagctcgtac
    16921 gtcccggcac caacgccatg cagcccaccc ccgtcatcgg cattcccacg cgcgacgcgc
    16981 acccggtcgt cgccgattgg tacgcgccca acggcaccga ccacggcaac gtcgaccgcg
    17041 tcatcgtcat gtcgcacggc ttcaagggcc accggcgctg gggattcatt cccatcgtcg
    17101 cgacccggct gcagttcaag ggcttcggcg tgctcgtcat cgatttttcg cacaacggcc
    17161 acgtgcccgc cgacggatcc gtggtcgaag agatcgcgga gggcttcgtg gcgccggagt
    17221 tgttccgcgc caacacgatc gcgcgtgagc tagacgacct cacgcacgtg ttgacgtggt
    17281 tgcgcgaccc gatgggcggc acgtttctgt tggggctcgc ggatcccgcg atcggcctgt
    17341 ggggacacag ccgcggcggc gtcatctcga ttctgaccgc gctcgacgac ccggggatcg
    17401 ccgcactggt cacctggtcc gcgctcgcgc accccgacca ttataccgag cgtcaaaaag
    17461 aactgtggcg aaccaagggc gagctcgcgt tcaccgacta tgcgagcgga accgcgctcg
    17521 cgttgggcga agcctatctc gacgacatcg aggcgcagcg cgacgcgtac gcagtcggcg
    17581 atcgcgccga acatctcacc atcccgcacc tgatcgtgca cggcgagcac gatcccaccg
    17641 tgccggtcgc ggactcgtac aagttctacg acacgcccac gattcgcgcg gacaagaagc
    17701 tcctgcgctt gctgacggga cacacgtacg gatacgacga ggggagaatc gcgagcgacg
    17761 cgctcgacaa cgccatcaag gccacagagg agtggttcga gacctatctc accgcgccgt
    17821 cggcgcagga atcgtagtcc gctagtcctt cttcgcgctt ccctggctgc gaaactccgc
    17881 gatccaattc tgcgcgacgc ggatcttgag catccgccg
//
LOCUS       DASPGK010000439         6456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_812093, whole genome shotgun sequence.
ACCESSION   DASPGK010000439 DASPGK010000000
VERSION     DASPGK010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812093"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VEC56_12310"
     CDS             <1..937
                     /locus_tag="VEC56_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HXV14980.1"
                     /translation="LHPELFHRHPLNPILTVADWPYPANTVFNPGATLLPDGTTLLLC
                     RVEDRRGHSHLSVARSANGIDEWAIDAKPTMSPDPERFPEEVWGIEDPRITFLPELKQ
                     YAIVYTAYSRVGPGVALALTKDFAEFERYGMIMVPEDKDAALFPRRIGGEWVLIHRPV
                     SAPGAHMWMSYSPDLRHWGGHKLMLEARVGGWWDANKIGLSPPPIETSQGWLVIYHGV
                     RQTSGGPLYRLGVALFDLHAPDRCIRRGSEWILGPEASYERRGDVDNVVFPCGYTLAA
                     DGDTIHLYYGAADTSIALATGSVRSVLDWLEQHGR"
     gene            complement(949..1497)
                     /locus_tag="VEC56_12315"
     CDS             complement(949..1497)
                     /locus_tag="VEC56_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXV14981.1"
                     /translation="METLVTEKSPPSKKVPAPARQAPDRGAEIDELLRIKQRHKFDAN
                     RVPCPCCGVVVEFTINRCPFCDSDIAAETALARETMRRLRELSGELDAEHAARRDGEE
                     APKKRGFFARLKYLFAGDPDPVDAPKVDPHAKRLLSNLAPGDSFKILAEDGPWVQVKT
                     MGGEIGWVYSTVRENTTSPRSR"
     gene            1691..4783
                     /locus_tag="VEC56_12320"
     CDS             1691..4783
                     /locus_tag="VEC56_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV14982.1"
                     /translation="MQSFPRSRTRLVFLTSLSLLALGWAVTPLEAEPPTPPPYYAIQN
                     VRVVTGVGAPIEGATVLLADGLIEAVGKNVKIPGDARVIDGKDLTLYPGLIDAMTTMA
                     QKQEPESSGERGRQAPAVMGPEDRPATTPWVTAVEQLADDAKIEKWRKAGFTAAVTTP
                     EKGIFAGQAALINLVDKPDRQAVLSTPVAQRVNFTGAGELRSFPGSLMGVLAYIEQTF
                     ADADHYSKVKAAYAKDPRGRVRPEFDRTLEPLETAVVDRTPFLMPANLGREIDRVLAL
                     QKELGIAAIVYGAQGAYARTEVLRTAKVPVLVNLNWPKEEKNRDPEAETPFRTLVHRR
                     MAPTTPAALAAAGVPFAFYSGGLASTSEVFESVRTATENGLSNEAALAALSANAAAIM
                     GVSDRVGTIEKGKIANLVLATDWPWADGAEVRAVFVDGRLYQERMDEGPSDPPAMDVG
                     GTWALSMTTPRGTREMTLDATMSKDGKVKGKMTSDRGESVLDDARMSGDVLRFKTTRE
                     VDGRSITSSWTLNVDGEKVAGTMSAGPMQADVTGSRTKKADADAVAKKTGDKPDVSAE
                     ELRDGVATYQGAAKKMGTFAITNANVWTVSGRTIENGTVVVSEGKIRAVGRDVKIPKG
                     AEVIDAQGGSLIPGIIDCHSHIAIEGGVNEGTLAVTAMVEIGDVLNPDDVAIYRALAG
                     GVTSANLLHGSANPIGGQNQVIKLRWGMDAEGLKFEGAPAGIKFALGENPKRSNFNAA
                     ANNVPIRYPQTRMGVMDVIREAFTEAREYQREWAVYKAGARNGIKPTLPRRDLEMEAL
                     VEILEGKRMVHSHCYRADEILQLLRLAEEFGFRVATLQHVLEGYRVADEIAEHGAGAS
                     TFSDWWGYKVEAYEAIPHNAALMTERGVVVSINSDSGEEMRHLNQEAAKAVKWGGMNE
                     VEALKMVTLNPARQLGVDRRVGSIEVGKDADLVLYDGHPLSTHSVVQKTFVDGDLYFD
                     IEWDQTRETTIAALKERLAGKSKKADAKETGAGAGAKSAPSPEVRWADEPYTCAEDHE
                     "
     gene            4780..6129
                     /locus_tag="VEC56_12325"
     CDS             4780..6129
                     /locus_tag="VEC56_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV14983.1"
                     /translation="MNRLNRVIVSLAVLIMWAATARAETLVIEGGTVHTMTGAPFVGR
                     VVIANGVIVGVGPDATVPPDAKRINAAGLHVYPGLCDAMCTLGLIEVDAVSATNDQAE
                     MGMYNPHLDAATAIHPASEVIPVTRANGVTHALVSPRAAREGVITGQATLVNLDGWTV
                     EEMAIDASAALVINWPGIVTRRFDSTTFSFKETPYEDAKEEAKKRQNELREWVEAARH
                     YRQAKGAEHSRAGADQKLAALAACLEGKKPVVIQADSKRDIEAAIAFADEAGLTMILA
                     GGAEAWKVKETLAEKKIPVILARTQSLPREEDDPYDRPYSNPAMLREAGVRIAFASGA
                     GGGGGGGSGAHSSRTIPYEAAMATGYGLSEEDAMKALTVWPAEIFGVADRLGSLEAGK
                     AANVIVTDGSPLEITSNVRHLIIAGREISTDNKHEASYEKYSSRPHRHPGSTAASRP"
     gene            complement(6212..>6456)
                     /locus_tag="VEC56_12330"
     CDS             complement(6212..>6456)
                     /locus_tag="VEC56_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV14984.1"
                     /translation="FNPATTIKYVLPSAQTVSLAIYDVRGRLVRALENGRRSSGPHTS
                     LWNGRDDAGQAVSSGVYYVRLAGESDIMTRKIVMLK"
BASE COUNT         1191 a         2118 c         2157 g          990 t
ORIGIN      
        1 tctacacccc gaactcttcc accgtcatcc gctcaatccc attctgacgg tcgccgactg
       61 gccgtatccc gcgaatacgg tgttcaaccc cggggctacg ctcttgcccg acggaaccac
      121 actgttgttg tgccgagtcg aggaccggcg gggccattct catctttccg tcgcccggtc
      181 cgccaatggc atcgacgaat gggcgatcga cgcgaagccc acgatgtcac ccgaccccga
      241 gcgctttccg gaagaagtgt ggggcatcga ggacccgcgt atcaccttcc tccccgagtt
      301 gaagcagtac gccatcgtct acaccgcata ctcgcgggtc ggaccgggcg tggccctggc
      361 gctcacgaag gacttcgccg agttcgagcg ttatggaatg atcatggtgc cggaagacaa
      421 ggacgcggct ctgtttcccc ggcgcatcgg cggagaatgg gtgctgatcc atcgcccggt
      481 cagcgctccc ggcgcgcaca tgtggatgtc gtactcgccc gacctgcgcc actggggtgg
      541 tcacaaactg atgttggaag cgcgcgtcgg cggatggtgg gacgcgaaca agatcgggct
      601 gtcaccgccg cccatcgaaa cctcgcaggg ctggctggtg atctatcacg gtgtgcgaca
      661 gacgtcgggc gggccgctct atcgtctagg agtcgcgcta ttcgatttgc acgcaccgga
      721 ccggtgcatc cggcgcggca gcgagtggat cttgggcccg gaggcgtctt acgagaggcg
      781 cggcgacgtc gacaacgtag tctttccttg cggctacacg ctcgcagccg acggcgacac
      841 cattcacctg tactatgggg cggcggatac gagtatcgcc ctggctacgg ggagtgttcg
      901 ctccgttctc gactggctcg aacaacacgg gcgataggca gcccgaggct atcgagaccg
      961 cggcgaagtc gtattctcac gcaccgtcga gtacacccag ccgatctccc cgcccatggt
     1021 cttgacctgg acccatggac cgtcctccgc gaggatcttg aacgagtctc ccggcgcgag
     1081 attgctcaat agacgcttgg cgtgcgggtc gaccttgggc gcgtcgaccg gatcggggtc
     1141 gcccgcgaag aggtacttga gccgtgcgaa gaacccgcgc ttcttgggag cttcctcgcc
     1201 gtcgcgccgc gccgcgtgct cggcgtccaa ctcgcccgac aactcgcgca gacggcgcat
     1261 cgtctcacgc gccaacgccg tctcggcggc gatgtccgaa tcgcaaaagg ggcagcggtt
     1321 gatcgtgaat tcgaccacga caccgcagca cggacaggga acccggttgg cgtcgaattt
     1381 gtggcgctgc ttgatgcgca ggagctcgtc gatctcggcc ccccggtcgg gcgcctgacg
     1441 ggcgggggcg ggcacctttt tcgacggcgg gctcttttcc gtgaccaatg tttccacgat
     1501 tctcacctcg aagggcaaac gacgacgagc gggcagaatg ttcctaccgg ggacgatccc
     1561 ggcgccgcga aaggtgcaaa atccgaacca acgggcgggg ctgctgcgcg cccctgcaaa
     1621 catgctatca tggtgcccgc caagcggcga acccgccgac cgtacctgga agcgcacaca
     1681 ggagtcaccg atgcaatcct tcccccggag ccgaacccgg ctcgtgtttc tgacgtcgct
     1741 ctcgttgctt gccttgggct gggcggtcac accgctcgaa gccgagcccc cgacaccgcc
     1801 tccttactac gcgattcaga acgttcgcgt cgtcaccggt gtcggcgcgc ccatcgaggg
     1861 cgcgacggtc ttgctcgcgg atggcttgat cgaagccgtc ggcaagaacg tcaagattcc
     1921 cggcgacgcg cgcgtgatcg acggcaagga tctcacgctc tatcccggac tcatcgacgc
     1981 gatgacgacg atggcgcaaa agcaggagcc cgagtcgtcg ggcgagcgcg ggcgccaggc
     2041 gcccgcggtc atgggacccg aagaccgccc cgccaccaca ccgtgggtca ccgcggtcga
     2101 gcagctggcc gacgacgcga agatcgaaaa gtggcgcaag gccggcttca cggccgcggt
     2161 cacgacaccc gagaagggga ttttcgcggg gcaggcggcg ctgatcaact tggtcgataa
     2221 gcccgaccgc caagcggttc tctcgacgcc cgtcgcgcag cgcgtgaact tcaccggggc
     2281 gggagaattg cgttcgttcc cgggctcgtt gatgggtgtg ctcgcgtaca tcgagcagac
     2341 gttcgcggac gccgaccact attcgaaggt caaggccgcc tacgcgaagg atccgcgcgg
     2401 ccgcgtgcgc cccgaattcg accgtaccct ggagccgctc gaaacggcgg tcgtggaccg
     2461 cacgccgttc ttgatgcccg cgaacctcgg ccgcgagatc gaccgtgtgc tggcgctcca
     2521 gaaggaactc ggcatcgcgg ccatcgtgta cggagcccag ggcgcgtatg cgcgcaccga
     2581 agtacttcgc accgcgaagg tcccggtgct ggtcaatctc aactggccca aggaagaaaa
     2641 gaaccgcgat ccggaggccg aaacgccgtt tcggaccctg gtgcatcgcc gcatggcgcc
     2701 gaccacgccg gccgcgctcg cggcggcggg cgtgccgttc gcgttctact cgggcgggct
     2761 tgcttcgacc tccgaagtat tcgagagcgt gcgcaccgcg accgagaacg gattgagcaa
     2821 cgaagcggcg ctggccgctc tctcggcgaa tgcggcggcc atcatgggtg tctccgatcg
     2881 cgtggggacg atcgagaagg gcaagatcgc gaacctcgtg ctcgcaaccg actggccgtg
     2941 ggccgacggc gccgaagtgc gcgctgtctt cgtcgacggc cgtctctatc aggaacgcat
     3001 ggacgaaggg cccagcgacc cgcccgcgat ggacgtcggc ggcacctggg cactctcgat
     3061 gaccacgccg cgcggcacgc gcgagatgac tctcgatgcc acgatgagca aggacggcaa
     3121 ggtcaagggc aagatgacga gcgatcgcgg ggagtcggtg ctcgacgacg cccgcatgag
     3181 cggcgacgtg ctgcgcttca agaccacgcg cgaagtcgac gggcgctcga tcacgtcgtc
     3241 gtggacgctc aacgtcgacg gtgaaaaagt cgccggcacg atgagcgcgg gcccgatgca
     3301 ggcggacgtc accggctcgc gcaccaagaa agcggacgcg gacgcggtcg cgaagaagac
     3361 cggcgacaag ccggatgtct cggcggagga gctgcgcgac ggcgtggcga cgtatcaagg
     3421 cgccgccaag aagatgggca ccttcgccat caccaacgcc aacgtgtgga ccgtctccgg
     3481 ccgcaccatc gagaacggaa ccgtcgtcgt ctcggagggc aagattcgag cggtgggccg
     3541 cgacgtcaag attcccaagg gcgccgaggt catcgacgcc cagggcggct cgctcatccc
     3601 gggtattatc gactgccact cgcacatcgc gatcgagggc ggggtcaacg aaggcacgct
     3661 ggccgtcacc gcgatggtcg agatcggcga cgtgctcaac cccgacgacg tcgctatcta
     3721 ccgtgcgctc gcgggcggag tcaccagtgc caacctgctg cacggcagcg ccaatcccat
     3781 cggcgggcag aaccaggtga tcaagctgcg ctggggcatg gacgccgagg gcctcaagtt
     3841 cgaaggcgcg ccggcgggca tcaagttcgc actgggcgag aacccgaagc gctcgaactt
     3901 caacgcggct gccaacaacg tgccgattcg ttatccgcaa acgcgcatgg gcgtcatgga
     3961 cgtgattcgc gaggccttca ccgaggcgcg cgagtaccag cgtgagtggg ccgtgtacaa
     4021 ggcgggcgcg cgcaacggca tcaagcccac gctcccgcgc cgcgacctcg agatggaagc
     4081 attggtcgag atcctcgaag gcaagcgcat ggtgcactcg cattgctacc gcgccgacga
     4141 gatcctgcag ctgctgcggc tggccgagga attcgggttc cgtgtcgcga ccttgcagca
     4201 cgtgctcgaa ggctaccggg ttgccgacga gatcgccgag cacggcgcgg gcgcgtcgac
     4261 cttctcggat tggtggggtt acaaggtgga agcctacgag gcgatcccgc acaacgccgc
     4321 gctcatgacc gagcgtggtg tggtggtctc gatcaattcg gacagcggcg aagagatgcg
     4381 ccacctgaac caggaagccg cgaaggcggt caagtggggc ggcatgaacg aagtcgaggc
     4441 tctgaagatg gtgacgctga accccgcgcg ccagctgggc gtcgaccgcc gcgtcggctc
     4501 gatcgaggtg ggcaaggatg ccgacctggt cttgtacgac ggccatccgc tttccacgca
     4561 ctcggtggtg cagaagacct tcgtcgacgg cgatctgtac ttcgacatcg agtgggacca
     4621 gacgcgagag acgaccatcg ccgcgctcaa ggagcgcttg gcgggcaaga gcaagaaggc
     4681 cgacgccaag gagacgggcg ccggtgccgg cgcgaaatcg gcgccctcac ccgaggtgcg
     4741 ctgggccgac gaaccctata cctgcgcgga ggatcacgaa tgaaccgcct caatcgagtt
     4801 attgtgtctc tcgccgtgct catcatgtgg gcagcgacgg cccgggcgga aaccctcgtc
     4861 atcgagggcg gcaccgtaca cacgatgacc ggggctccct tcgtgggccg cgtggtgatc
     4921 gccaacggcg tcatcgtcgg cgtgggtccc gacgccaccg tgccgcccga cgcgaagcgg
     4981 atcaacgccg ccggcctgca cgtgtacccg ggattgtgtg acgcgatgtg cacgctggga
     5041 ctgatcgaag tcgacgccgt ctcggccacc aacgaccagg ctgagatggg catgtacaac
     5101 ccgcacctcg acgcggccac ggccatccat cccgcgagcg aagtgatccc cgtcacccgc
     5161 gccaacggcg tcacgcacgc gttggtgtcg ccgcgcgcgg cgcgcgaagg cgtgataacg
     5221 gggcaggcga cgctcgtcaa tctcgacgga tggacggtcg aggaaatggc gatcgacgct
     5281 tcggccgctc tggtgatcaa ctggcctggc atcgtgacgc ggcgtttcga ctcgacgacc
     5341 ttcagcttca aggagacgcc gtacgaggac gccaaggaag aagcgaagaa gcggcagaac
     5401 gagctgcgcg aatgggtgga ggcggcgcgc cactaccgac aggcgaaagg ggccgagcac
     5461 tcgcgcgccg gcgcggacca gaagctggcg gcactcgccg cgtgcctcga gggcaagaag
     5521 ccggtcgtca tccaggcgga ctccaagcgc gacatcgaag ccgcgatcgc gttcgcagac
     5581 gaggccgggc tcaccatgat cttggccggc ggcgcggaag cgtggaaggt caaggagacg
     5641 ctggccgaaa agaagatccc ggtgatcctc gcgcgtaccc agagcctccc gcgcgaagag
     5701 gacgacccct acgaccgccc ctactccaac ccggccatgc tgcgcgaggc gggagtgcgc
     5761 atcgcgttcg cgtcgggcgc gggcggcggt ggcggcggcg gatcgggagc tcactcgtcg
     5821 cgcaccatcc cgtacgaggc cgcgatggcg acgggctacg gcttgtcgga agaggacgcc
     5881 atgaaagcgc tgacggtgtg gcccgcggag atcttcggcg tcgcggatcg gctgggatcg
     5941 ctcgaggcgg gcaaggccgc caacgtaatc gtgaccgacg gcagcccgct cgagatcacc
     6001 tcgaacgtgc gccacctgat catcgccggt cgggagattt cgaccgacaa caagcacgag
     6061 gcctcgtacg agaaatacag ctcgcgaccg caccgacacc cagggagcac cgccgcgtcg
     6121 cgaccgtagc gacgcgatct tcttcgaaac gaaacgcccg gacgtcttcg cgacgcccgg
     6181 gcgtttttct tcgccgcgtt ccgcgtgagc cttacttcaa catgacgatc ttgcgcgtca
     6241 tgatatcgct ctcgcccgcg agtcgtacgt agtacacgcc cgacgacacc gcttggcccg
     6301 cgtcgtcgcg tccgttccac agcgaggtgt gcggcccgct cgaacgcctg ccgttttcga
     6361 gcgcccttac cagacgtcca cgcacgtcgt aaatcgccag cgaaaccgtc tgcgccgacg
     6421 gcagcacgta cttgatggtg gtcgccgggt tgaacg
//
LOCUS       DASPGK010000440         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1395794, whole genome shotgun sequence.
ACCESSION   DASPGK010000440 DASPGK010000000
VERSION     DASPGK010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395794"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VEC56_12335"
     CDS             complement(<1..754)
                     /locus_tag="VEC56_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXV14985.1"
                     /translation="MKILLTGGTGFVGSGVRRELRAQGHDVRLLARPQSASKIDPREG
                     VEVVVGEVLDTQACLRATKDCDALVHLVGIRREDPQRGITYEALHTEATYAVVDAARR
                     NGVERAVLMSSLGTRKDAVSGYHRTKFESEEIVKKSGMRWTIFRPSVIFGPGDEFHPL
                     IADLVHRPIVPVIDGGKALIQPVAHHNVVGPMARTLTMPETQGKVFEIGGPERHAFID
                     ILYKIARHYGVWPNTMAVSSRFAKPMVKFAQRF"
     gene            complement(751..1113)
                     /locus_tag="VEC56_12340"
     CDS             complement(751..1113)
                     /locus_tag="VEC56_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14986.1"
                     /translation="MCADPVCSECFSPLFNTVICSNHEGLDDEGEWELVALYATEGPV
                     NSVRYFLDDHAVPSIAVETDEGTVELYVPIAEKDEAWASLDGGEAGDDMLKCDECRVY
                     YAREVGECPICGGGSEAR"
     gene            complement(1166..2431)
                     /locus_tag="VEC56_12345"
     CDS             complement(1166..2431)
                     /locus_tag="VEC56_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXV14987.1"
                     /translation="MIDSHCHLNLQHFEDDREATIARAVSDGVVGFVNIGYDRDSLRE
                     TLELVERYPFVFGAAGVHPHDAATLDDELDAQVRHALDHPRIVAVGEIGLDFYRNLAP
                     REVQIETFRRMIKLARERDKPIVIHCRDAFNEVLDVLESEGGTYRGIFHAFSAGEAEA
                     RRVSELGFHVGIGGVATYRNARLGETVAAIPLERIVLETDSPYLTPHPWRGKRNEPSF
                     VTHVARTVARAKGLSPAEIDRATTENTLAAFGLSPDALPRPVYRIEAAAYIQAAAADP
                     ADLDAVPTEGVSEAIITGFSDPLERIEHVLALATRARERGWRVRVNTTGLANHAAGRD
                     VTAELKGIVDEVNVVLFGASARAHDELAYPAVGNEGWASIRDFVRCSVASGLETVCEF
                     IAVPGFEPEPCREFTRELGATYDIRMYRS"
     gene            complement(2435..>3196)
                     /locus_tag="VEC56_12350"
     CDS             complement(2435..>3196)
                     /locus_tag="VEC56_12350"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXV14988.1"
                     /translation="IGKDITRFHCLIWPAMLMAAGVELPKSIFGHGFVYNRGEKMSKT
                     LGNIVDPINLANYFGADALRYLLLREIAFDRDGDFTVELFVTRYNAELANELGNLFSR
                     TLSMTQRYFEGAVPAYTPEGAKEAERLTRTVAQDYRREMDALAFDRALAAVWRAVQEG
                     NRFVEERKPWAQAKNEREALGDTLRALLEVLRMCSILVQPFMPEKALAMRAQLGLSAG
                     FTLDEAGRFGDGAWKRIGTSAVLFPRIEIDAKKAS"
BASE COUNT          524 a         1075 c         1064 g          533 t
ORIGIN      
        1 ggaaacgctg cgcgaacttg accatgggct tggcgaagcg cgacgacacc gccatcgtgt
       61 tgggccacac accgtaatga cgcgcgatct tgtacaggat gtcgataaag gcgtggcgct
      121 ccggcccgcc gatctcgaac accttgccct gcgtctccgg catggtgagg gtacgcgcca
      181 tagggccgac aacattatgg tgcgccacgg gctggatcag ggccttgccg ccgtcgatga
      241 cggggacgat ggggcgatgg acgaggtcgg cgatcagcgg gtgaaactcg tcgccgggtc
      301 cgaagatcac cgagggacga aagatagtcc agcgcatgcc cgacttcttg acgatctctt
      361 cggactcgaa cttggtgcgg tgatagcccg acacggcgtc cttgcgcgta cccaggctgc
      421 tcatgaggac cgcgcgctcg acgccgttgc ggcgcgccgc gtcgacgacg gcgtaggtcg
      481 cctcggtgtg gagcgcttcg taggtaatgc cccgctgcgg gtcctcgcga cggatgccga
      541 ccaggtgcac gagcgcgtcg cagtctttgg tggcgcgcag gcacgcctgc gtgtccagca
      601 cctcgcccac cacgacctcg acgccttcgc gcgggtcgat cttggatgcg ctctgagggc
      661 gtgcgagcag gcgcacgtcg tggccctggg cgcgcaattc gcgccgcacg cccgacccca
      721 cgaaaccggt tccgcccgtc agcaggatct tcatcgcgct tcgcttcctc ctccgcagat
      781 cgggcactcg ccgacctcgc gggcgtagta gacgcggcac tcgtcgcact tgagcatgtc
      841 gtcgccggcc tcgccgccgt cgagcgacgc ccaggcttcg tccttctcgg cgatggggac
      901 gtaaagctcc acggttccct cgtcggtctc gaccgcgatc gagggcaccg cgtggtcgtc
      961 caggaaatag cgcacgctat tgacgggccc ctcggtggcg tacaacgcca ccaattccca
     1021 ctcgccctcg tcgtcgaggc cttcgtggtt cgagcagatc accgtgttga acaagggcga
     1081 gaagcactcg ctgcacacgg gatcggcgca cacgccgcat gtcccgacga gagtcccgtc
     1141 ggaatgatca catttcattg caccctcacg aacgatacat ccgaatgtcg tatgtggcac
     1201 ccaactcgcg cgtgaactcg cggcacggct ccggctcgaa gccgggcacc gcgatgaact
     1261 cgcacacggt ctcgaggccc gaagccaccg agcagcgcac gaagtcgcga atcgaggccc
     1321 acccttcgtt tcccaccgcg ggatacgcca gctcgtcgtg cgcgcgcgcg gacgcgccga
     1381 acaggaccac gttcacctcg tcgacgatac ccttcagctc cgcggtgacg tcgcggccgg
     1441 ccgcgtggtt cgcgagtccc gtcgtgttga cccgcacgcg ccaaccgcgt tcgcgcgcgc
     1501 gcgtcgccag ggcgagcacg tgctcgatgc gctccagggg atccgagaag ccggtgatga
     1561 tcgcttcgct gacgccttcg gtcggaacgg cgtcgaggtc ggcggggtcg gccgcggcgg
     1621 cctgaatata cgcggcggcc tcgatcctgt aaaccggcct cggcagcgcg tccggggaca
     1681 ggccgaacgc ggccagggtg ttctcggtgg tggcccggtc gatctcggcc ggcgacaacc
     1741 ccttggcgcg cgcgacggtc cgcgccacgt gggtgacgaa ggagggttcg ttgcgcttgc
     1801 cccgccaggg gtgcggcgtg agatagggcg aatcggtctc gagcacgatg cgctcgagcg
     1861 ggatggccgc caccgtctcg cccaggcgcg cgttgcggta cgtcgcgaca ccgccgatgc
     1921 cgacgtggaa gcccagctcg gagacgcgac gcgcctcggc ttcgccggcc gagaaggcgt
     1981 ggaagatacc gcggtaggtc ccgccctccg actccagcac gtcgagcacc tcgttgaagg
     2041 cgtcgcggca atgaatgacg atgggcttgt cgcgctcgcg cgcgagcttg atcatgcgcc
     2101 ggaaggtctc gatctggact tcgcgcggcg ccaggttgcg atagaagtcg aggccgatct
     2161 cgcccaccgc gacgatacgc gggtggtcga gcgcgtggcg aacctgggcg tcgagctcgt
     2221 cgtcgagcgt ggctgcgtcg tgcgggtgca cgccggcggc cccgaacacg aacggatagc
     2281 gctcgaccag ctcgagcgtc tcgcgcagcg agtcgcggtc gtagccaata ttgacaaacc
     2341 ccaccacccc gtcggagacg gcgcgggcga tggtggcttc gcggtcgtcc tcgaagtgct
     2401 ggaggttcaa atggcagtgc gagtcgatca tgggctagct cgccttcttc gcgtcgattt
     2461 cgatgcgcgg gaaaagcacg gccgatgtgc cgatgcgctt ccacgccccg tcaccgaacc
     2521 ggccggcttc gtccaaggtg aacccggccg acaggcccaa ttgcgctcgc atcgcgagcg
     2581 ctttctcggg catgaacggc tgcacgagga tcgagcacat tctcagcact tccagcagcg
     2641 cgcgcaaggt gtcaccgagc gcttcgcgct cgttcttggc ctgcgcccac ggtttgcgct
     2701 cttccacgaa gcggttgccc tcttggaccg cgcgccaaac cgccgcgagc gcgcgatcga
     2761 aggcgagcgc gtccatctcg cgtcggtagt cctgcgccac ggtgcgggtc aggcgctcgg
     2821 cctctttcgc gccctccgga gtatacgcgg gcaccgcacc ctcgaaatag cgctgcgtca
     2881 tcgagagcgt gcgcgagaag agattgccca gctcgttggc gagctcggcg ttgtagcggg
     2941 tcacgaacag ctccacggtg aagtcgccgt cgcggtcgaa ggcaatctcg cgcaataaga
     3001 ggtagcgcag cgcgtccgcc ccgaagtagt tcgccaggtt gatggggtcg acgatgttgc
     3061 ccagcgtctt gctcatcttc tcgccccgat tgtacacgaa gccgtgcccg aaaatactct
     3121 tgggcagctc gacgccggcc gccatcagca tcgcgggcca gatcaaacaa tggaagcgcg
     3181 tgatgtcctt gccgat
//
LOCUS       DASPGK010000441         9860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1162589, whole genome shotgun sequence.
ACCESSION   DASPGK010000441 DASPGK010000000
VERSION     DASPGK010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162589"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VEC56_12355"
     CDS             <1..599
                     /locus_tag="VEC56_12355"
                     /inference="COORDINATES: protein motif:HMM:NF025274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HXV14989.1"
                     /translation="HPRFRTPYKTTIVTGVCVALLASVANINEVVELTNIGTLFAFVL
                     VCIGVTVLRFQEPERVRPFRVPLGPFLFPMLGVVSCVFLMVHLPPASWWRFIGWLVLG
                     MSIYFAFGYNHSAVGRTHGRPEKPTGVQRVVASGFLLAGLGLFVIPHDAGPSELLTLA
                     MDSNARAAAGLTMIAVGVAAAIAGGFRMGTHLSSTPRA"
     gene            610..2292
                     /locus_tag="VEC56_12360"
     CDS             610..2292
                     /locus_tag="VEC56_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HXV14990.1"
                     /translation="MNLTVLAIGFIVLLVAAYRLYGGWVARHFSLDDARETPAHTHAD
                     DVDFVPIRPFYLFAQHFSAIAAAGPIAGPILACQAFGWLPCLIWISLGVVLIGAVHDF
                     STLAASVRHGARSIAEIAREHLGTRAGRALMCFIWIALVYVIVAFADITAATFVVGSE
                     ELQQGGVTFNPGGAVAAASVMYLALSVVLGLVQRYLKPPLWLATILFVPATFAVAWAG
                     THLSHVLILDHKTWGVLILAYCALASVVPVWALLQPRGYLGGFVLYSALIVGVVGLLF
                     GGYSVQQPAFKGWDVGGMTGTLFPFLFVTIACGACSGFHGLVCSGTTCKQIAKESHIR
                     PIGYGAMLAEGFVALIALTTIMIAAPNAIQGLAPGTIYGNGIGQFLTILIGEDKLAFA
                     VTFGAMAFSTFVFDTLDVSMRLGRYLLQELFGWRARAGAFAATVATVAIPCYFIAFGE
                     KGTWVKFWTLFGASNQLLAALSLLVITVWLHQARQRIAFTLLPMLFVLTITLWSLAKL
                     LVANVRAANGFDIAFVNAVAAAALTLLALFLVASALVKVRDERRAGRIPQPA"
     gene            2506..3543
                     /locus_tag="VEC56_12365"
     CDS             2506..3543
                     /locus_tag="VEC56_12365"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXV14991.1"
                     /translation="MATNKNPELTAPKGPAWIQAVKKYRRPHHGRSAWQLINTLGPYL
                     LTWYLMVRSLEVSYWLTLALIPIASGFLIRSFIISHDCGHGAFFRSRKANTITGTITA
                     LLVFTPYEQWRHEHAVHHASGGDLDHRGIGDIWTMTVEEYMQAPRARRFRYRLHRSPW
                     VMLTIGPLLQFLVLQRFARRPMTKREHVSIHQTNVLIFAVVLVMCLTIGWKAYLLIQL
                     PTMFLAGVVGVWLFYVQHQFEGVQWARHEEWDFAKGAVDGSSFYKLPRVLQWITGSIG
                     YHHIHHLSPKIPNYYLEQCYKENEIFQNVQHITLRSSLKSLAFRLWDEEQQKLVGYAG
                     VKAYRERHGLT"
     gene            3727..4689
                     /locus_tag="VEC56_12370"
     CDS             3727..4689
                     /locus_tag="VEC56_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV14992.1"
                     /translation="MIRARMVLCTLLVAAAAPALAANWTEKPIVRAGFGTGADQFGYA
                     GIQSSKKTTRLVSCFAASRDHIAVHDRIKKDVKLFTAAGVFERAIPLALKGAGADTVR
                     ARDIALDGDRLIVLVDDLPAGSKEFPASMRLAVFDLPSAACIEVRTIDTSSLIPPGKS
                     PARGTDAFCLHPNGDSVWIFDTIRQMSFEVAGTEIGKKPRYGWGGERMVRTDDNVGAI
                     LLIDGEGKLVRRLPESGVITAVTESGDGFAVMQSAGGADWAVVVYNGAGDRLGVAPRP
                     NRVWQPFKAPVMERKYELTDTPAGPELYEMYANADGVRVVRWSK"
     gene            4766..7303
                     /locus_tag="VEC56_12375"
     CDS             4766..7303
                     /locus_tag="VEC56_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HXV14993.1"
                     /translation="MRPHVPILISLVGCALCASSALADGGATSATATLITSNPYVDSG
                     NTTGKGNNALLGACGSGGSDTAEDGWYRLQLTTPAVLELWITCGPGGYDTRMAVFDST
                     LAMVACNDDDPGCGAPYYRSRISNLSVGPGRYYVVVDGYSGQEGPYTLNVAWAPPCEG
                     DSGPNNPTLVGTIPFTEASTTVGTCNDVPIECELGPGGSAPDRWYSVSLDTTVLLDVW
                     TDCTPSGLDTRVGVFTAGLSSVYCNDDDPACANGQSRIASAFLPAGDYFVVVEGAGAT
                     SGAFTIHIDTTVVDPSANPALLPDIIVRQDDLYDNQFVTSGVPPRTYIRFSNGTANVG
                     DGKFHVYGTGVDNGDGTENIMQRIYYPGGGFIDRTAGQFVFHPSHNHIHVEEWCEYRV
                     RQVLPNDGVGDIVVKGAKTSFCILDLDVYDPTLPNYDPAGQFFSCSSTVQGLSVGWID
                     IYDKSLSGQSIDITGVPAGTYWLESHADPNDDFLERDETNNTSRVKFTIGGGGSINPD
                     PYETNESFSAVAARTVGIPNSPNLGPCDPVRTITGLTCHVANDLDYFRFYATATGTPD
                     DFVRIDFTNANLNLYLLDASGTTIASSETIGSFERIELDGRPAGWYFVLARPHLPSTV
                     ASYSLTINPPANAAPSIDVIEPAAGTQFRIQGAETFTVTWNATDPESGPMWATVFANT
                     SPVFDGNEFLLPTGLNVDASLGFHVVNTAYLAIDTYWFYVSVSDGGTTLGEWSDGTLK
                     LLDGTTDVDLPSVARTSLHVAVPNPFNPTTTLRLDLRVGGPVQWDIFDVHGRRVRAIH
                     SGSLGAGVHERMWDGHDDAGAPVASGVYFQRVVTPDGVFRNKLVLLK"
     gene            complement(7308..9734)
                     /locus_tag="VEC56_12380"
     CDS             complement(7308..9734)
                     /locus_tag="VEC56_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HXV14994.1"
                     /translation="MRLQSLFLLLVVLAAGPARSEWTWRYPQPQGHTLYDIVFLTNTT
                     AIAVGEAGTVLVTTDAGQSWVVSFQVAGVETHLRRIDRLDASSAVAVGDDGVVLRTTD
                     GGDTWTPIASGTSVDLLDVSFGDAMNGIAVAGVTVLRTGNGGLAWQSTSVPSFLRAVD
                     MITATDAIAVGDWTDVMMTTNGGADWTPKPEPLLPPYNAPLTSVDFIDALNGVVGAAL
                     GDDGWVSFNSAWHVTSDGGDTWAPLYPFGGAVEEYTPNEILYPAVGQIFAAYRMTCCR
                     LSSYPFSDWGEFVAGGAPFAHRNITHSANGLARNDDGLVLVVGDDGLILRRNPDGTFV
                     RTGGVLHREYLAGGWSASSFADPSTGIVMASDDYMYPASGSPETHFARTTDGGLTWAS
                     TTLANVQSKDLAHLSADEVYTIGYDVGGRGIAARSVNGGASWLTVWTSPTIFPGAIAS
                     GSPTHAVAVCSGGWALVIDNGTVTPVAAGVASTDVAFATPSVVVAAGGNTVRRSLDGG
                     LNWTALPNPIQPIRALDFATPSTAFAVSTTGILRSDDQAQSWQVVPSGAHAGLRAIDV
                     SGSGHGVAVGDDAVALITSDGGDTWIPFPPPTNGDFNRVTVFSDGRAFASGPPQTLFE
                     FLQQTVPTLISRFDVEARPFGADLRWEVRDDADLASFSIQRLLDARAETIAAGLSRGA
                     RSYRDDGVLPGRTYQYQLIAVDRDGAMTVSAPISATIPSAALELLQNHPNPFNPSTRI
                     SFVVPAKERIRLDVYDATGRRVATLVDEVKDVGVHTVEWNGSGSASGVYFARLESGKD
                     SVSRKLVLLK"
BASE COUNT         1610 a         3294 c         3165 g         1791 t
ORIGIN      
        1 tgcacccgcg cttccggacc ccctacaaga cgaccatcgt caccggggtg tgcgtggcgc
       61 tgctggcgtc ggtggccaac atcaacgagg tggtcgagct caccaatatc gggacgctgt
      121 tcgcgttcgt gctggtgtgc atcggcgtca cggtgctccg ttttcaagag cccgagcgcg
      181 tgcggccatt tcgcgtgccg ctgggaccgt tcctatttcc catgctcggg gtggtgtcgt
      241 gcgtgttctt gatggtccac ttgccgccgg cgtcgtggtg gcgattcatc ggttggctgg
      301 tgctcggaat gtcgatctac ttcgcgttcg ggtacaacca cagcgcggtc ggtcgcacgc
      361 acgggcggcc ggagaaaccg accggcgtgc agcgcgtggt cgcgtccgga ttcctgctcg
      421 cgggactggg gctgttcgtg attccgcacg atgcgggacc gtcggagctc ttgacgttgg
      481 ccatggactc caacgcgcgc gcggccgcgg gactgaccat gatcgcggtg ggtgtggccg
      541 ccgctatcgc gggcgggttc cgcatgggga cgcacctctc gagcacgccg cgggcgtaat
      601 cgtgcgggaa tgaatctcac cgtcctcgcg atcgggttca tcgtgctgct ggtggcggcg
      661 taccgcctgt acggcggctg ggtggcgcgg cacttctcgc tcgacgacgc gcgcgagacg
      721 cccgcgcaca cgcacgcgga cgacgtcgac ttcgttccca ttcgtccctt ctacttgttt
      781 gcgcagcact tttccgctat cgcggcggca gggcccattg cggggcccat cctggcgtgc
      841 caggccttcg gttggttgcc gtgcctcatt tggatcagct tgggtgtggt tttgatcggc
      901 gccgtgcacg acttctcgac cttggccgcg agcgttcgcc acggcgcgcg ctcgatcgcg
      961 gagatcgcgc gcgaacacct gggcacgcgc gcgggtcgcg cgctgatgtg cttcatctgg
     1021 atcgcgctgg tgtacgtgat cgtcgccttc gccgacatca cggcggccac cttcgtggtc
     1081 ggcagcgaag agctgcagca gggaggcgta acgttcaacc cgggcggggc ggtggccgcg
     1141 gcgagcgtca tgtatctcgc cctctcggtg gtcctcggcc ttgtgcagcg ctacctgaag
     1201 ccgcccttgt ggctggcgac gatcctcttt gtgccggcga ccttcgcggt ggcgtgggcc
     1261 gggacgcacc tatcgcacgt cctgatcctc gatcacaaga cctggggcgt gctcatcctt
     1321 gcgtactgcg cgctcgcgag tgtggtgccg gtatgggcgc tcttgcagcc gcgcggttac
     1381 ctgggtggtt tcgtgctcta cagcgcgctg atcgtgggcg tggtcggttt gttgttcggc
     1441 ggctattcgg tgcagcagcc cgcgttcaag gggtgggatg tgggcgggat gaccggtacc
     1501 ctgttcccgt ttctcttcgt gaccatcgcg tgcggcgcgt gctcgggctt ccacggcctc
     1561 gtgtgctcgg ggacgacgtg caagcaaatc gcaaaagagt cgcacatccg gcccatcgga
     1621 tacggtgcca tgctggcgga gggattcgtc gcgctgattg cgctcaccac catcatgatc
     1681 gcggcgccca acgcgatcca gggcctggcg ccggggacga tttacggtaa cggcattggg
     1741 cagttcctga ccatcctcat cggcgaagac aagctggcgt tcgcggtcac cttcggcgcg
     1801 atggcgtttt ccactttcgt gttcgacacg ctcgacgtgt cgatgcgcct ggggcgctat
     1861 ctgttgcagg agctgttcgg gtggcgcgct cgcgcgggtg ccttcgcggc caccgtcgcg
     1921 acggtggcga tcccgtgcta cttcatcgcg ttcggcgaga aggggacgtg ggtcaagttc
     1981 tggaccctct tcggggcgtc gaatcagctg ctggccgcgc tctcgttgct cgtcataacg
     2041 gtttggctgc accaggcgcg ccaacgcatc gcgttcacgc tcctgccgat gctgttcgtc
     2101 ctgacgatca cgctgtggtc gttggccaag cttctggtgg cgaacgtacg cgccgcgaac
     2161 ggcttcgaca tcgccttcgt caacgccgtc gccgcggccg ccctgacctt gctggcgctc
     2221 ttcttggtgg cctccgcgct ggtcaaagtt cgcgacgagc gcagggccgg ccggattccg
     2281 caacctgcct agaatcagcg tgtcgcggtg gtttacgacg cgatttgtgc ttaccggatg
     2341 gggggttccg gtaattaata gctcgacacg tagagagatg tcggcctata ttgagtacag
     2401 gtagccggac gcacaccggt gcgcaaagga accttcgaga taaatgtcgg ggccttgcat
     2461 gatgcagggc cccgtttttg cttcagaccg aaacgagacc gttctatggc gacgaacaag
     2521 aacccagaat tgaccgcccc caaggggccc gcatggatcc aggcggtgaa gaaataccgc
     2581 cgcccacacc acggccgcag cgcctggcaa ctcatcaaca cgctcggccc gtacctgctg
     2641 acctggtacc tgatggttcg atcgctcgag gtgtcgtact ggctgaccct cgcgctgatc
     2701 ccgatcgcat ccggcttcct gattcgctcc ttcatcattt cgcacgattg cggccacggt
     2761 gcgttcttcc gctcgcgcaa ggcgaatacc atcacgggca cgatcaccgc gctgctggtc
     2821 tttacgcctt atgaacagtg gcgccacgaa cacgcggtcc atcacgcctc cggcggagac
     2881 ctcgatcacc gcggcatcgg cgatatctgg accatgacgg tcgaggagta catgcaagcg
     2941 ccgcgcgcgc gtcgtttccg ttaccgcctg catcgcagcc cgtgggtgat gctcaccatc
     3001 ggcccgctcc tccagttcct ggtgctgcag cgcttcgcgc gccgtcccat gaccaagcgg
     3061 gagcacgtga gcatccatca gaccaacgtg ctcatcttcg cggtcgttct ggtaatgtgc
     3121 ctgacgatcg gctggaaggc gtatttgctg atccagttgc cgacgatgtt cctggctggc
     3181 gtggtgggcg tgtggctgtt ctacgtccag caccaattcg aaggcgtgca gtgggcgcgc
     3241 cacgaggagt gggacttcgc caagggcgcc gtggacggca gctcgttcta caagttgccg
     3301 cgcgtgctgc aatggatcac gggcagcatc ggctaccacc acatccatca cttgagcccg
     3361 aagatcccca actactatct cgagcagtgc tacaaggaga acgagatctt ccagaacgtg
     3421 cagcacatca cgctgcgttc gagcctgaag tccctcgcgt tccgcctgtg ggacgaggag
     3481 caacagaagc tggtcggcta cgccggcgtc aaggcgtacc gggagcggca cggcctcacg
     3541 tagggattgc ctcgatgcga tggtccggga tcgcgatcgt cccccggcct cgattcttgc
     3601 cagatagacg ctgccgctcg ctccgggggt gccctccggc atgggggttg ccaggccagc
     3661 cttacactgg cattcctcgg cctcaacccg tactattccg cctggacgac cttcctggag
     3721 gggttcatga ttcgcgcccg catggtattg tgcactctgc tcgtcgccgc cgcggcccct
     3781 gcgctcgccg ccaactggac cgagaagccc atcgtgcgcg ccgggttcgg caccggcgcc
     3841 gaccaattcg gctacgcggg cattcaatcc agtaagaaga ccacgcgcct ggtgagttgc
     3901 ttcgccgcga gccgcgatca catcgcggtt cacgaccgca tcaagaagga cgtcaagctc
     3961 ttcaccgccg cgggcgtgtt cgagcgcgcg atcccgctcg cactcaaggg tgcgggtgcg
     4021 gacacggtac gagcgcgcga catcgccctc gacggtgatc gactgatcgt cctggtcgac
     4081 gatcttccgg ccggctccaa ggaattcccg gcctcgatgc gtctcgccgt gttcgacctc
     4141 ccgagtgccg cgtgcatcga ggtgcgcacc atcgacacgt cgtcactcat cccgcccggc
     4201 aagtcgcccg cgcgcggcac cgacgcgttc tgcctccacc ccaacggcga ctcggtgtgg
     4261 atcttcgaca ccattcgcca gatgagcttc gaggtggcgg gcacggaaat cgggaagaag
     4321 ccgcgctacg gctggggagg cgagcgcatg gtgcgcaccg acgacaacgt gggcgcgatt
     4381 ctcctgatcg acggcgaagg caagctggtg cgacgcctgc ccgagtccgg ggtcatcacc
     4441 gccgtcaccg agtcgggcga cggcttcgcg gtcatgcaga gcgcgggcgg cgccgactgg
     4501 gcggtcgtgg tctacaacgg cgccggcgat cgtttgggcg tggcaccgcg ccccaatcgg
     4561 gtctggcagc ccttcaaggc ccccgtaatg gagcgaaagt acgagctcac ggatacgccc
     4621 gcgggccccg agctctacga gatgtatgcc aacgccgacg gcgtgcgtgt ggttcgctgg
     4681 tctaagtaag cggccgcaag gccgctggtc gtagggcctt gtcgcgtgct ataatgagac
     4741 gccttcgacg gtgccatcac cgatcatgcg accccacgtc ccaattctca tctctctcgt
     4801 aggttgcgcc ctgtgcgctt cttccgccct cgcggacggc ggggccacca gtgcgacggc
     4861 gacgctcatc acgtccaatc cctacgtcga ttccgggaac acgaccggca agggcaacaa
     4921 cgccctgctg ggcgcgtgcg gttccggcgg ctccgacacg gccgaggacg ggtggtaccg
     4981 gctccaattg acgacaccgg cggtcttgga attgtggatc acgtgcggcc ccggcggtta
     5041 tgacacgcgg atggccgtgt tcgacagcac gctggcgatg gtcgcgtgca acgacgacga
     5101 ccccgggtgc ggcgcgccgt actaccgctc gcgcatttcc aacctctccg tggggcccgg
     5161 gcgctattac gtcgtcgtcg acgggtactc cggccaagag ggtccctata ccctcaacgt
     5221 cgcgtgggcc ccgccgtgcg agggcgactc cggtcccaac aatccgaccc tcgttggcac
     5281 catcccgttc accgaggcca gcacgacggt cggcacgtgc aacgacgtcc cgatcgaatg
     5341 cgagctcgga cccggcggct ccgcgcccga ccgctggtac agcgtctcgc tcgacaccac
     5401 ggtgttgctc gacgtgtgga ccgattgcac gccgtcgggg ctcgatacgc gcgtcggcgt
     5461 cttcacggcc ggactctcgt cggtgtactg caacgacgac gaccccgcgt gcgcgaacgg
     5521 gcagtcccgt atcgcgtccg cctttctccc cgcgggggac tacttcgtcg tggtcgaagg
     5581 tgcgggtgcc accagcgggg cgttcacgat ccacatcgac accaccgtcg tcgacccgtc
     5641 ggcgaacccc gcgctgctcc ccgacatcat cgtgcgccag gacgacctct acgacaacca
     5701 gttcgtcacc agcggcgtgc cgccgcgaac gtacattcgc ttttccaacg gaaccgccaa
     5761 cgtgggcgac gggaagttcc acgtgtacgg aaccggcgtc gacaacggcg acggcaccga
     5821 gaacatcatg cagcgcatct actatccggg cgggggcttc atcgatcgca ccgcgggcca
     5881 gttcgtcttt cacccgtccc acaaccacat ccatgtggaa gagtggtgcg agtaccgcgt
     5941 gcgccaggta ctcccgaacg acggcgtggg cgacatcgtc gtcaaggggg cgaagaccag
     6001 tttctgcatc ctcgacctcg acgtctacga tcccaccctc cccaactacg atcccgcggg
     6061 acagttcttc tcgtgcagct cgacggtgca ggggctctcg gtgggatgga tcgacatcta
     6121 tgacaagtcg ctgtccggcc agagcatcga catcaccggc gtaccggcgg ggacctattg
     6181 gctggagtcg cacgccgacc cgaacgacga tttcctcgaa cgcgacgaga ccaacaatac
     6241 ctcgcgcgtc aagttcacga tcggcggcgg cgggtcgatc aatccggacc cctacgaaac
     6301 caacgagtcg ttctccgcgg tggcggcgcg cacggtcggc attcccaata gccccaacct
     6361 cggtccctgc gacccggtcc gaaccatcac gggactcacg tgccacgtcg cgaacgacct
     6421 cgactatttt cgcttctacg ccaccgcgac cggcacgccc gacgacttcg tgcgcatcga
     6481 cttcaccaac gcgaacctga acctgtattt actggacgcg tcgggcacga ccatcgcctc
     6541 gtcggagacc atcggttcgt tcgaacggat cgagctcgac ggacgcccgg ccggctggta
     6601 cttcgtgctc gctcgcccgc acctaccgag cacggtggcg tcgtattcgc tcaccataaa
     6661 cccgccggcc aatgcggcgc cgtcgatcga cgtgatcgaa cccgcggccg gtacgcagtt
     6721 ccgcatccaa ggtgcggaga ccttcaccgt gacctggaac gcgaccgacc ccgagagcgg
     6781 gccgatgtgg gccacggtgt tcgccaacac gtcaccggtg ttcgacggca acgagttcct
     6841 gcttcccacc ggactcaacg tggatgcgag cctgggattt cacgtcgtca acaccgcgta
     6901 tctcgccatc gacacctact ggttttacgt gtcggtcagc gacggcggca cgacgttggg
     6961 cgagtggtcc gacggaacac tgaagctcct cgacggcacg acggacgtcg acctcccgtc
     7021 ggtggcgcgg acgtccctgc acgtcgcggt ccccaacccc ttcaatccga ccaccaccct
     7081 gcgcctcgac ttgcgcgtcg gcggccccgt gcagtgggat atcttcgacg ttcacggccg
     7141 ccgcgtgcgc gcgatccatt ccggttcgct gggtgcgggc gtccatgagc gcatgtggga
     7201 cggccacgac gacgccggcg ccccggtcgc cagcggcgtg tatttccagc gcgtcgtcac
     7261 cccggacggc gtctttcgca acaagctggt cctgctcaag tagtcggtta cttgagcagc
     7321 accagcttgc gtgacaccga gtccttgccc gactccaagc gcgcgaagta cacgccgctc
     7381 gccgatccgc tcccgttcca ctccaccgtg tgaactccaa cgtccttcac ttcgtcgacg
     7441 agagtcgcca cgcggcggcc ggtcgcgtcg tagacatcca gtcgtatccg ctccttggcc
     7501 ggtactacga acgagatcct cgtcgacggg ttgaacgggt tgggatggtt ctgcagtagc
     7561 tcgagagccg cgcttgggat cgtcgccgaa atcggggccg acaccgtcat cgcgccgtcg
     7621 cgatcgaccg cgatcaattg atactggtag gtccggcccg ggagcacgcc gtcgtcccgg
     7681 tacgaccgcg ccccgcgcga gaggccggcc gcgatcgtct cggcgcgcgc gtccaggagg
     7741 cgctgtatcg agaacgatgc cagatcggcg tcgtcgcgaa cttcccagcg caggtcggcg
     7801 ccgaacggcc gcgcctcgac gtcgaagcgg ctgatgaggg tcggtaccgt ttgctgcaag
     7861 aactcgaaga gcgtctgcgg cggccccgac gcgaaggcac ggccatcgga gaagaccgtt
     7921 acccggttga agtcgccgtt cgtcggcggg gggaacggaa tccacgtgtc accgccgtcg
     7981 gaggtgatca gcgcgacggc gtcgtcgccg accgcaaccc cgtgaccgct gcccgagacg
     8041 tcgatggcgc gcaagcccgc gtgagcgcct gaaggcacta cctgccagct ttgtgcctgg
     8101 tcgtcgctgc gcagtatgcc cgtggtggag acggcgaacg cggtcgacgg agtggcgaag
     8161 tccagcgcgc ggatcggctg aatcgggttg ggcagcgccg tccaattgag accgccgtcc
     8221 aggctccgac gtaccgtgtt cccgcccgcg gccacgacca ccgaaggagt cgcgaacgcc
     8281 acgtcggtcg aggcgacgcc cgccgccacg ggcgtcacgg tcccgttgtc aatgaccaac
     8341 gcccagccgc cgctgcatac cgcgaccgcg tgggttggcg aaccggacgc gatagcgccg
     8401 gggaagatgg tcgggctcgt ccacaccgtc aaccacgagg cacccccgtt gaccgaacgc
     8461 gccgcaatcc cgcggcctcc cacgtcgtag ccgatggtgt agacctcgtc ggccgagagg
     8521 tgtgcgaggt ccttggactg cacgttggcg agggtcgtgg acgcccacgt cagccctccg
     8581 tcggtcgtgc gcgcgaagtg cgtctcgggc gagccgctcg cggggtacat gtagtcgtcg
     8641 ctggccatca cgatacccgt cgatgggtcg gcgaacgacg acgcagacca tccgcctgcg
     8701 aggtactcgc gatggagaac gcctccggtg cgcacgaagg tgccgtcggg attgcgccgc
     8761 agtatcaatc cgtcgtcccc gacgacaagc acgaggccgt cgtcgttgcg cgcgagcccg
     8821 ttcgccgagt gcgtgatgtt gcgatgagcg aacggcgcgc cacccgccac gaactcgccc
     8881 cagtccgaaa agggatagga actcaagcgg cagcacgtca ttcggtacgc cgcgaagatc
     8941 tgacccaccg ccggatacag aatctcgttg ggcgtgtatt cctcgacggc gccgccgaac
     9001 ggatacaggg gcgcccaggt gtctccgccg tcactcgtta cgtgccaggc gctgttgaat
     9061 gagacccacc cgtcgtcgcc gagggcggcc ccgacaactc cgttgagcgc gtcgatgaaa
     9121 tcgacgcttg tgaggggagc gttgtacggg ggcaacaggg gctcgggctt cggcgtccag
     9181 tccgcgccac cgttcgtcgt catcatgacg tctgtccaat ctcccacggc gatcgcgtcc
     9241 gtagcggtga tcatgtccac ggcgcgcaga aacgaaggaa cgctcgtgct ttgccaagcc
     9301 agaccgccgt tgccggtacg caaaacggtg acgccagcca ccgcgatgcc gttcatcgcg
     9361 tcgccgaagg agacgtcgag aagatccacg gacgtcccac tggcgatggg cgtccaggtg
     9421 tcgccgccgt cggtcgtgcg caggacgaca ccgtcgtcgc ccaccgcgac ggcggacgac
     9481 gcgtcgaggc gatcgattcg gcgcagatga gtttccacac ccgcgacttg gaacgatacg
     9541 acccacgact gaccggcgtc ggtcgtgacc aggaccgttc ccgcttcgcc cacggcgatc
     9601 gcggtggtgt tggtcagaaa aacgatgtcg tagagcgtgt gaccttgtgg ttgcgggtat
     9661 cgccacgtcc actcggaacg agcaggaccg gcggccaata cgacaagaag gaggaagagc
     9721 gattggagtc tcatggtggc ctccccgggt cagtaggatg tgagaaacat aatggtacca
     9781 cacacccgtt gtcggtggac acccgcgaac cccacgttga cccggcccgg cacctatgtt
     9841 attctcattg ccgttgttat
//
LOCUS       DASPGK010000442         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_578253, whole genome shotgun sequence.
ACCESSION   DASPGK010000442 DASPGK010000000
VERSION     DASPGK010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578253"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="pth"
                     /locus_tag="VEC56_12385"
     CDS             <1..430
                     /gene="pth"
                     /locus_tag="VEC56_12385"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXV14995.1"
                     /translation="LMEPLTYMNLSGDVLAPWIAQESLPLDRLLVVCDDFALPLGSLR
                     LRKNGSDGGHNGLASIIERLDSLEFPRLRLGVGPVPPRVDPAEFVLAEFLPEEERTVD
                     EMLVVAADCIEAWVMHGIDKAMSRFNTRKEPPAEAPGEAP"
     gene            539..949
                     /gene="rpsF"
                     /locus_tag="VEC56_12390"
     CDS             539..949
                     /gene="rpsF"
                     /locus_tag="VEC56_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXV14996.1"
                     /translation="MSTGRYSVRSYECAVILHPAVNDDGLRASTAKYVEIIAKGGGEI
                     TKVETWGKRRLSYEIMKQPEGTYFFYRFRGGNALVDELGRQMRIDENVLRHMIVRDEL
                     AEGNEPALEPGTVEPTMRHPEREREEVEHRGERY"
     gene            993..1208
                     /gene="rpsR"
                     /locus_tag="VEC56_12395"
     CDS             993..1208
                     /gene="rpsR"
                     /locus_tag="VEC56_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXV14997.1"
                     /translation="MRKKKCKFCLDRVTHIDYKDERRVRRFMTDRGKITPRRITGTCA
                     KHQRMLAAAVKRARLIALVPYVREYYR"
     gene            1222..1665
                     /gene="rplI"
                     /locus_tag="VEC56_12400"
     CDS             1222..1665
                     /gene="rplI"
                     /locus_tag="VEC56_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXV14998.1"
                     /translation="MEVILLESIHKLGDRGQAVKVKPGYARNYLFPRKLALPATPANR
                     RVFQENERVLIKRDIAAVETARARAAKYVDVAVNIAVQVGEEDKLYGSVTALDIARKL
                     KEQGHDVERREIELAEPIRQIGVYNVDLKFHREVSVPIKVWVVKE"
     gene            1673..2743
                     /locus_tag="VEC56_12405"
     CDS             1673..2743
                     /locus_tag="VEC56_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXV14999.1"
                     /translation="MIAVVLAGGVGERFWPSSRRRRPKQLLDLTGRGSMLQLTLDRLD
                     GLASREDTFVMTFAEQRDAVLAALGGRVPDANVIGEPEIRNTAPSIALAAAIARRTRG
                     DQAMLVLPADHVVEPAERFREQVRAAVRYLARADDLLTFGIVPTRAESGYGYIRLGDE
                     RWSEGQDRIHAAAGFVEKPAPEKARELVAGGSLWNSGMFVWRAGAVLDGIAHHAPDLA
                     AVLAPIEAAVGTPGFEGVLKREYPRAPSISIDYALMEKARNVVVMRADFAWNDVGSWE
                     FVRDVGVPDADGNVVVGEHVVVDAKDNTVVAPGRVVAMIGVEGLVVVDGGDAILVCRR
                     DRVQDIKQIVAELKRRGRGDLV"
     gene            2763..>3240
                     /locus_tag="VEC56_12410"
     CDS             2763..>3240
                     /locus_tag="VEC56_12410"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXV15000.1"
                     /translation="MSWIRNLLPMVALVALVATGCSKKTETAKVDTSEEELAARVGDW
                     SITREFVEEYLRRMPEPQRQKFDSPEGRALLAEKLMQEELAYLEAKKLDLASRETVRA
                     RIEEATRSILVAEYLDEYVDSKARPTDEEMHDYYDAHQDMYTQLETIRAQHVFSKDK"
BASE COUNT          587 a         1015 c         1138 g          500 t
ORIGIN      
        1 gctcatggag ccgctcacct acatgaatct ctcgggtgac gtgctggcgc cgtggatcgc
       61 gcaggaatcg ctgccgttgg atcggctctt ggtcgtgtgc gacgacttcg cgttgccgct
      121 ggggtcgttg cgcctgcgca agaacgggag cgacggaggc cacaacggct tggcgtcgat
      181 catcgagcga ctcgactcgc tcgaattccc gcgcttgcga ctcggtgtcg gccccgtgcc
      241 gccgcgggtc gacccggccg aattcgtcct ggccgagttt ctcccggaag aggaacgcac
      301 cgtggacgaa atgctggtgg tggccgccga ctgcatcgaa gcctgggtga tgcacggtat
      361 cgacaaggcc atgagccgct tcaatacccg caaggagccg cccgcggagg cgccggggga
      421 ggctccttga cgcgcggccc cgcatcgcct atagtgtgcc gcacatagtc cttagccaga
      481 tcggctcaaa cgacttctct accccccggc cggccttcgg tcgctgcggg ggggtcacat
      541 gtccacaggg aggtactccg tgagaagtta cgagtgcgcc gtgatcttgc atccggccgt
      601 caacgacgac ggtctgcgtg cgagcacggc caagtacgtc gagatcatcg ccaaaggcgg
      661 cggtgagatc accaaggtcg aaacctgggg caagcgtcgt ctctcctacg agatcatgaa
      721 gcagcccgag ggcacctatt tcttttatcg gttccgcggc ggcaacgcgc tggtcgacga
      781 gctgggccgg cagatgcgca tcgacgagaa cgtgctgcgg cacatgatcg tgcgcgacga
      841 gctggccgag ggcaacgagc ccgcgctcga gccgggtacg gtcgagccga ccatgcgtca
      901 ccccgagcgc gagcgtgagg aggtcgagca ccgtggcgag agatactaag cgcgatccca
      961 agaagaaggg cgagaagcgc gaaaagcgcc cgatgcggaa gaagaagtgc aagttctgcc
     1021 tcgaccgcgt cacgcacatc gactacaagg acgaacgccg cgtgcgtcgt ttcatgaccg
     1081 accgcggcaa gatcacgccg cgccgcatca cgggaacgtg cgccaagcac cagcgcatgc
     1141 tggcggcggc ggtcaagcgc gcgcgtctga tcgcactcgt gccgtacgtg cgcgagtact
     1201 accggtaaag gagagccgat cgtggaagtc attctgctgg aatccatcca caagctgggc
     1261 gaccgcggcc aggccgtgaa ggtcaagccc ggctacgcgc gcaattactt gttcccgcgc
     1321 aagctcgcgc tccccgcgac accggccaac cgccgcgtgt tccaggagaa cgagcgcgtg
     1381 ctgatcaagc gcgacatcgc ggcggtcgaa accgcgcgcg cgcgcgcggc caagtacgtc
     1441 gacgtggcgg tcaacatcgc ggtgcaggtg ggcgaggaag acaagctgta cggctcggtc
     1501 accgccctcg acatcgcgcg caagctcaag gagcagggtc acgacgtcga gcggcgcgag
     1561 atcgagctgg ccgagccgat ccgtcagatc ggcgtgtaca acgttgatct caagttccac
     1621 cgcgaggtgt cggtcccgat caaggtctgg gtggtcaagg agtaagggac gcgtgatcgc
     1681 ggtagtactc gcgggtggtg tcggtgagcg gttttggccg tccagccgcc gccgtcgccc
     1741 caagcagctg ctcgacctca ccggtcgggg cagcatgctg cagttgacgc tcgaccgcct
     1801 ggacgggctc gcgtcgcgcg aggacacctt cgtcatgacg ttcgccgagc agcgcgacgc
     1861 ggtgctggcg gcactgggag gccgcgtccc cgacgcgaac gtcatcggcg agccggagat
     1921 tcgcaatacc gcgccgagca tcgcgctcgc ggccgcgatc gcgcgccgca cccgcggcga
     1981 ccaagcgatg ctggtgctcc cggccgacca cgtggtggaa ccggcggagc gctttcgcga
     2041 gcaggtgcgc gcggcggttc gatacctggc ccgtgccgac gacctgctca cgttcgggat
     2101 cgtcccgaca cgggccgaga gcggatacgg atacattcgg ctgggcgacg agcgctggag
     2161 cgaagggcaa gaccgcatcc acgcggcggc cggcttcgtc gaaaagcccg cgccggagaa
     2221 ggcgcgcgag ctggtggcgg gcggctccct ctggaacagc ggcatgttcg tgtggcgggc
     2281 gggggcggtg ctggatggga tcgcgcacca cgcgccggac ctcgccgcgg tgctggctcc
     2341 gatcgaagcg gcggtgggaa cgccgggctt cgagggggtg ttgaagcggg agtatccccg
     2401 cgcgccgtcc atctcgatcg attatgccct catggagaag gctcgcaacg tcgtcgtgat
     2461 gcgcgcggac ttcgcctgga acgacgtggg gagctgggag ttcgttcgcg acgtgggagt
     2521 acccgatgcc gacggcaacg tcgtggtggg cgagcacgtc gtggtcgacg ccaaggacaa
     2581 caccgtggtg gcccccggtc gcgtggtcgc aatgatcggc gtcgaggggc tggtcgtggt
     2641 cgacggaggc gacgccattc tggtgtgccg tcgcgaccgt gtgcaagaca tcaagcagat
     2701 cgtcgccgag ctcaagcgcc gtggtcgcgg agacttggta tgacgaggag gttcgtttcg
     2761 ctatgagttg gattcgcaat ctgctgccga tggtagcgct ggtcgcgctg gtcgcgacgg
     2821 gctgttcgaa gaagaccgag accgcaaagg tcgacacttc cgaggaagag ctggcggccc
     2881 gcgtcggcga ttggtccatc acgcgcgagt tcgtggaaga gtacctgcgc cgcatgcccg
     2941 agccgcagcg gcaaaaattc gactcgccgg agggccgcgc actcctggcc gagaagttga
     3001 tgcaggagga gctcgcgtac ctggaggcca agaagctgga cctcgcctcg cgcgagaccg
     3061 tacgcgcccg catcgaggaa gccacgcgct cgatcctggt tgccgaatac ctggacgagt
     3121 acgtcgactc caaggcgcgc cccaccgacg aggagatgca cgactactac gacgcccacc
     3181 aggacatgta cacgcagctg gaaaccatcc gcgcgcagca cgtgttctcg aaggacaagg
//
LOCUS       DASPGK010000443        19543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1632736, whole genome shotgun sequence.
ACCESSION   DASPGK010000443 DASPGK010000000
VERSION     DASPGK010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 19543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19543
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632736"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VEC56_12415"
     CDS             complement(<1..342)
                     /locus_tag="VEC56_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15001.1"
                     /translation="MPRRTLLILAVWLTAAPGVRAADGLTIESIFGSRSLLKPIPPTV
                     EWLGNSRGVSFVRKTQTDPPRSELVLREVPSGRERVILVADTVTVAADLRPDHPTFSI
                     GSHQWNRSGDRA"
     gene            653..3457
                     /locus_tag="VEC56_12420"
     CDS             653..3457
                     /locus_tag="VEC56_12420"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXV15002.1"
                     /translation="MFRKLGLLAVACFMVVSAEAASAGQTLIEPSATALEVDAPAAPA
                     ATPAKAEANKGMKPRPYAVELQKKLKKPGPPPSKPKFKPWDEIVTKDHKKVEGLLTLH
                     TKGAEVLLELKKDDLDKPMLAILSLSQGIGSNFVYGGLPIDDVMFDFHRDEDNIQLRR
                     LTANFRAEGDEELQKAIDLTFAESILESFPIKTEREGKVIIEVKDFFLSDVAGMSIWL
                     NGALSQPMRLEAKKGYFGSIKNYPTNTEIDTRLTYAASRPEALFRPNFPDPRAIQVGV
                     AWSIRKLPEEPMTPRIADDRVGYFTTSFKDFTKEKTETFFTHYANRWRLEKKDPAAAV
                     SEPKQQIVYYIDHTVPKEYVPYMIAGVNMWQKAFEAAGFKEAIVGKPAPTKEEDPNYD
                     PVDARYNTIRWNVSDQVSYGAIGPSRVDPRTGEIIDADILFEHNIVHNFGKSWRRVAA
                     PRPALMEVDHNLKQLWMTPEEREREEAIMSLPFFKGRPYMLCSVNDCMGLGAEMMRLT
                     MLINGMIEAGQEVPMEYIGAALTFVSAHEVGHTIGLRHNFKSSGAVPYDQLNDKAKID
                     QIGMTGSVMDYPTPNIAANASQQGYYYTPIVGTYDMWAIEWAYKPIDGATPEEQSKNL
                     EAIARKCVDKAYLYGTDEDTYPMGALDPRSNTNDLSDDPMRWANERMSICNDLLMKGQ
                     LEGRVVAEGGDYVPLRAAVQALFVQKYVCSNIATKTVGGAYTERAHKGDGKAPFTLIT
                     ADEQRKALQFVVDNALKSNAYALSPDMLNKMQDDKMWSWENNLFQQGRRFDFPLSDWV
                     EAMQTGVLFNVMNPFLQARVVDNAYKNNSAFKLSDLYSTLTKSIWTDNLTPNGRTATW
                     DRNLQRVYTDMLIQQMVEPYPVTPQDAVSLSRLNLTRIKTAAQSGLQRKGLDDETNAH
                     LMETIARVDRALNASREANF"
     gene            complement(3549..3929)
                     /locus_tag="VEC56_12425"
     CDS             complement(3549..3929)
                     /locus_tag="VEC56_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15003.1"
                     /translation="MFVIRTLMIIALALMTAAVVIGCSGKVATKSPSSRDVIIVKDEP
                     GPPDHAPAHGHRKKQGNQSVILEYDERMDLYVVSGYDDCYYTAGQFYRKVKGAWEWSV
                     CIDGPWKAVAHRNDLPPGLRKHKA"
     gene            complement(4041..5840)
                     /locus_tag="VEC56_12430"
     CDS             complement(4041..5840)
                     /locus_tag="VEC56_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15004.1"
                     /translation="MTFSSTCKRALTAAALGGVLAACDDSVVYPPGPEQFAFVTEPAE
                     IGALHGVTVTPTGARAAGLNGLVIDWDGNLWSSYPTGTNAEIFGIGSASDGTVFAVGR
                     DGLILRDTGTGFASLASGTTESLNSVSGVRSNSAFAVGNAGVIRYIQSASVIDWPSNT
                     TQNLRGVWMASDTVAVAVGNGGTALHFDGSSWNPRPTPTTENLRAVFGFAEDDVFAVG
                     NTARILHYDGGSWQQMSQPSGITSRALLGVWGQTPSDVFACGEAGVMLHYNGSTWTRF
                     TNTIFTSQNFEGITGTAGDDVIAVGAGGTIVRYDGNEWKKELSDVGSTAVYRGIWGTS
                     ANDVFMVGPFAQVLHRAGGVWRFMVGGRPQQLNDVSGTSPAHVIAVGDQGLIMGYDGA
                     AWTTQTSGTAEGLNGVWAAAANEAWAVGDNGTVLHLSGGTWNPQTSGTTKVLHAVWGV
                     SASNVYAVGNDGLVLNYDGGNWSEHATLNAIALRGIWGASASNIIAVGTGAPGQSSAF
                     RYNGSSWQPESISTVSALESVFGVSSSDIFVVGLGGRHQNNFYDAFFNYDGEAWIRSQ
                     ASTFGSYTDVWAPSGSTAFVVGTGAIVYRGTKN"
     gene            complement(5899..7653)
                     /locus_tag="VEC56_12435"
     CDS             complement(5899..7653)
                     /locus_tag="VEC56_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15005.1"
                     /translation="MRPRYSIAIPLTVALACLLACDDDPIAPSPPEASEPQWSEWREL
                     PPSFTVYSMWAVGPNNVFAVGPAGTAARWNGVRWTSVPVDLFRNVWSVTGTPDGSVVA
                     VGDGGATYSFNGIAFAPTPQVTAEDLRSVWAASDDTLLAVGELGTLLVGNGSTWSLAP
                     PPVRASMLSVWGTSMRDVFVVGPDGLIIHFDGNAWSEQSSGTLETLASVSGTASNDVY
                     AVGTSGTILHYDGADWSPMTSPTGSVLQCVVAGAGDPIAVGANGVALLLQDGAWSRVE
                     VGTTHWLYAACRAGTHTWVGGSRVVRVHDGTAWRSEATGAVPILRGVGADPSGSAIVV
                     GDDGYIARGRGDGWEVDEGVDARDLYCVYLTSGGELFAGGVQRLLRFDGTEWVVEIDD
                     IITWYGFAESPTELYAVGSNGTLRRRSGGGWTTEIMPRFTESLNAVACLSGSEAYAVG
                     DRGVVLRYDGIGWTQLNSRSLVDIRDVIEAPGDDLNRRALAIGESGALFVLNTQGVTA
                     LESPTSANLYALARDPAGDILAFGGGASMLRYRDGTWSSDDSPVLQPLYAAHSGETEV
                     FVVGGGVSGGLVLRYGPP"
     gene            complement(7729..8577)
                     /locus_tag="VEC56_12440"
     CDS             complement(7729..8577)
                     /locus_tag="VEC56_12440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV15006.1"
                     /translation="MDGKTVLVTGASSGIGKATAAGLSALGARVVMVSRTQERGLRAR
                     AEILRTMPGASLEVVVADLSTTAAIRRLADVVAHKCPRLDVLVNNAAVLTSRRKLTSE
                     GFEMQFFVNHLAYFLLTGLLLDVVRASAPARIVNVASTAHSRGVIDFDDLQLAEAYRG
                     WQAYANTKLMNILFTYELARRLEGTRVTANVLHPGVIHTNLLSNFSSVMQLAWHAVGR
                     WFKQPEDGAETPVYLASSPEVEGVSGKYFRHCKPMGTSAQSNDREIQRRLWKESERLS
                     GFTYPI"
     gene            8610..9806
                     /locus_tag="VEC56_12445"
     CDS             8610..9806
                     /locus_tag="VEC56_12445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXV15007.1"
                     /translation="MSEATAKARAIIHVDMDAFYASVEVMDDPALAGRPVIVGGTRES
                     RGVVSAASYQARVFGVRSAMSTARAERLCPHGVFIAPRMDRYAEISHDIRLILDAYTP
                     LVEPISIDEAFLDVTGSQALFGTGPEIGRAIKRRIRDEIGLVASVGVAPNKYLAKLAS
                     DLEKPDGFVVITQEEAEARLAPLPVWRLWGVGKKTEEAFAKAGLRTIADVTRAPDALL
                     EQLTGSYAPRLKQLARGIDDRPVVPDADAKSIGAENTFARDIADGGALRAELDALSER
                     VARRARADDVVGFTVNLKARYADFTTVTRAKTLPDATADSVVIRDVARDLLEKKLDRQ
                     GRALRLLGVSLSNLARAEETSLDLFESNLRAPATRNRTLDAVMDSLRARFGSSAVRRG
                     TKRGPR"
     gene            complement(9840..12152)
                     /locus_tag="VEC56_12450"
     CDS             complement(9840..12152)
                     /locus_tag="VEC56_12450"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HXV15008.1"
                     /translation="MHGPRRFSLHFNTRRALLAASLIFLACGEDGDDYAPLGNGETPQ
                     ATVVAGLVRDQDRSAVADAVVVIEPANEGIPASADFLSKNPDAASASTPGRRVTTTND
                     HGRFAFEDVEAGEYYLQVIADDHLGGMQSVRVPDRMAFVDTVYVDVNLTPTGTFSGVA
                     ALENAAIHQGTVVYVEGTSYVAVTDPTGDYAITNVPVGSYTVRAEHAGYLEDTESGTI
                     TTAGENVSLAGMLLRINSNIPPVANVGNATPLFEDIPVNFTGSGSDADGTIARYEWDF
                     EDDGTFDYSSLTTASTSHVYPEPGAYRAKLRVTDDDGGIGLDVIELDIVPNDPTVVYM
                     SPSGSDGNDGTIATPVLTLARAYQVAQQTAKTVILAREGTYNGQVPAFLPGIDVMGGR
                     TPQWDESPSGYSTFNFTLNRALANNITTPTLIRRVSLYMSQPATGTNSIALYSVGSDA
                     DLQFQDCRFDVDGALSGNSGANGTTGANASSGFTGQAGSCDGARGLGGAGGASPGGCP
                     GGAGGAGGVEGPNDGFPGIAGACTGGAGGVGGQGDAGSGGLTCDAVGGPGGNGSTGAV
                     GISGTDGAAASPNGSIIANEWVPNTSGSGLVGAPGDGGGGGGGGGGQGGTFCNDGGGN
                     GGGGGGGGGSGGTGGTGGVGGRAAFCVMLVNSSPTFDGCTFLRGIGGTGGGGGFGGSG
                     GNGGFGGSGATACLGEVGRGGNGGAGGDGGQGGGGAGGPGGPSYGIYRVGASNPVTIG
                     NTFSGGAGGTGGLGASGAPNGPTGLFGTIN"
     gene            complement(12179..12793)
                     /locus_tag="VEC56_12455"
     CDS             complement(12179..12793)
                     /locus_tag="VEC56_12455"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXV15009.1"
                     /translation="MSRRALRASMAPAGVLALLLFLGAAPCRAGSICGTVRDGVTLQP
                     VPNAAVFLFDNLDQYTGLYAGTDVDGRYCIDNIPAGTYTIQVRVDNYLSAVVGGIVVE
                     EDATGVDVTTRAPLFLDQPWPNPATNEVTFRLDAPDGVETRLEVYDVRGRLVMGWQGD
                     GPASTRTIRWDLRDASGAPVASGIYLIRLRAGGNEIVRRFVRLR"
     gene            complement(12879..14627)
                     /locus_tag="VEC56_12460"
     CDS             complement(12879..14627)
                     /locus_tag="VEC56_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15010.1"
                     /translation="MTHFRHSSAALAAALLLVSCSDDPVGPGHGGSSTTPYQASMDYF
                     DSPIVSLWGGPAGDVYASGSSIMRLDGDTWEVIALPNADDGLLTTWGTSSGDLYAMGY
                     SDMHRFDGAQWHRIARPAQIGDVWGADNGEIFVVGGGNHRLYHYDGSDWFVDSLKVDD
                     RGDPWHLVSGASPDDVYIAGYAGWIGHFDGLGWNASRPRAQHYYQSIWKAPDGPLYLA
                     TYDSLFTYDGTEMTPVDLGMSLWSPRIFGRSADEVYCAAEFDYPTTSLLRFDGSAWSR
                     AADIDRYVRVVWGDQSSNALYAAGDVVWKIAGGEATPSLGDPELFGYDFMDLWGSEED
                     GIYVVGTRAYRYHEGTWTDLKKQDLTSARAYSVWGRSGREIYAVGPSMILEYDGASWT
                     AVSGGAGKELNSVAGNDREIFAVGYNGAIVRHDGDSWAPMESGTSYHLFAVYAWKDGA
                     FAGGQNGALMRYDGREWRPFPSPVGWHILDMFGFGPNSIFAVGSNSTEICRFDGRVWQ
                     AIFIGYTRGSNYSVWGTSEHDVFLGQGRGDVLHFDGSNWSYLPRVTTRPVRSVWGAPG
                     REIIAATEGGVVRYHR"
     gene            complement(14687..16573)
                     /locus_tag="VEC56_12465"
     CDS             complement(14687..16573)
                     /locus_tag="VEC56_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXV15011.1"
                     /translation="MIQFNKVTKSFGAQRLFDDLSFAVNRRERVGLVGRNGHGKTTVF
                     RMLLGDVTPDSGDIVVPKRYRIGHLDQHIRFTQPTLLDEAALGLPPDHSDDLWRAEQI
                     LFGLGFGEDDLARAPELFSGGFQVRLNLAKVLVSEPDLLLLDEPTNYLDVVSIRWLER
                     FLNEWKSELVLITHDRSFMDKVVTHTVAIHRRKAKKIAGDTGKLYAQILQEEEVYEKT
                     RMNEDRKRKDIERFIERFRAKNTMATRVQSRIKMLAKHERLDKLEHLQTLEFSFRAQA
                     FPAKVMMKVDHVSFGYGDAPELISDLRFEIHDRDRICVIGPNGRGKSTLLRMLAGELA
                     PRSGAITSHPRTVSGYFAQTNVARLDERLTVAEEIQSTVPMTSPQIARDIAGAMMFEG
                     DNALKKIAVLSGGERSRVTLGKLIVTPCNLLLLDEPTNHLDMDSCDSLLAAIDAFDGA
                     AVIVTHNEMFLHTLATHFVVFDRGRVRVFDGSYQDFLDTVGWEMDEKLTKPSTAAKLA
                     PAPAAKPAPPPPPAAPIDRKANRQARAKMIQERSRKLAPLEKRVAEIEARIESLERER
                     DETFRLLSEASATGNASAIAELSKKSREVGPKIDAAYEHLESATRDLERETKAFDERE
                     KSAG"
     gene            complement(16570..18168)
                     /locus_tag="VEC56_12470"
     CDS             complement(16570..18168)
                     /locus_tag="VEC56_12470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV15012.1"
                     /translation="MRPIPRLSASPRERMWFAAILVLALALRLWDLGARSLWFDEASE
                     YWVATAPLSQLAHFVSVGSGDPPLFSFLLHFWMKVGTNESWLRLLSVIASLAGVAGVM
                     VLGRRLGGVPAAVAAGVLAAINPPDIRYAQEVGQYALMMGTVSWSLVALHGLWNETGR
                     RWVIGWAAVAFLATTAYYASVFCVLVPCGCALVEALVRRDRARSRRLALALGIYALVT
                     APVLWAVLPDQLERVLNTRAALAEYPQQRPEGIALVWRWLANLFGFFFTGFPFSRVPT
                     WIPVVSALALLGLAMRARPRWTLWFAAAWAAYGIAGLLEIFPFGFRWGLILCPPALVL
                     VSIGFTMGARDRALKLFGALAFTGLIACSLVSLPNRTVRDAIDPQRTVQWPETEDMRP
                     VVEYWHDKWSRSQPTYVFYGAAPAFAYYLQRYPDTRGDLPPTWNLACWHDDTPPDFCR
                     SGNIYYGRWLRSLPTPEAKIKSLSQTIVDQPREFWIVFSHVHGSESADMMVRIKQNGY
                     VMADWVERRAAGAVLMRLERPSSP"
     gene            complement(18176..18967)
                     /locus_tag="VEC56_12475"
     CDS             complement(18176..18967)
                     /locus_tag="VEC56_12475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV15013.1"
                     /translation="MAAARPVALVTGASMGLGAEFARLLASEGYDLVVTARSADRLGA
                     LKKEIEGLHGVGVRVVVHDLADPRAPVAIYDDVRAAGLEIDVLVNNAGFGMYGLFHQS
                     DLQTELDMIQVNIAAVVHLTKLFARDMVARKRGRILNVASTAGFQPGPLQSVYYASKA
                     FVLSFTEAIANELRGTGVTATALCPGPTPTEFQKRAHVGKVRGLRLMMRINPDVVVRA
                     GYAGMMKGKAVVVPGALNNVMAFTVRFFPRRMVTAVVRRIQTASD"
     gene            complement(18967..>19543)
                     /locus_tag="VEC56_12480"
     CDS             complement(18967..>19543)
                     /locus_tag="VEC56_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXV15014.1"
                     /translation="LSLHSCTKYMNGHNDIVAGAVIGRADLVEQVRLNLNHLGGSLDP
                     HACYLLHRGVKTLALRVRHQNVSALAIARFLDEHPEVASVNYPGLIGNLHHARAAEFF
                     DGFGGMLSFHLKGGARAADRLLERVELPEIAPSLGGVDSLMTIPAKTSHRLLSPEERE
                     RIGIGDGLVRLSVGIEATDDLIDDLRQALES"
BASE COUNT         3477 a         6561 c         6396 g         3109 t
ORIGIN      
        1 ggcccggtcg ccggagcgat tccactgatg gctgccgatc gagaaagtcg ggtggtccgg
       61 gcggaggtcg gcggccacgg tgaccgtgtc ggccaccagg atcacccgct cgcggcccga
      121 agggacctcg cgaaggacga gctcggatcg gggcgggtcg gtctgggtct ttcggacgaa
      181 cgacaccccg cggctgttcc ccagccactc gacggtcggc ggtatcggct tgagaagcga
      241 gcgcgaaccg aagatcgact cgatcgtgag tccatcggcc gcgcggacac cgggggcagc
      301 cgtcagccac acggccagga tgagtagtgt gcgtcgaggc atgatttctt cgacctctcc
      361 gaagggcccc agcgaccgaa tgaagggccg gggagccaac agcttagcac gataccggtt
      421 gaatcccacc cggtttccct ctatcatgcc cccgggtggc tgaaacgcgg taacgcggtg
      481 actttccctc tcgagtccgc tctctcacgg ggcccgtgcc cgggaatcga acccgtcccc
      541 gacggcggga tgacgtaaag aaacacgccg gtgcgatgga acgaattcgc ctcggcgctc
      601 gtactagcct gcaacccacc cagcattttc tataacagga ggttagagga gcatgtttcg
      661 gaaactcggg ctgctcgcgg ttgcgtgctt catggtcgtc tcggccgaag ccgcctctgc
      721 cggccaaacc ctgatcgagc ccagcgccac cgcgctcgag gtcgacgcgc cggccgcgcc
      781 ggcagcgacc cccgcgaagg ccgaggccaa caaaggtatg aagccgcggc cgtacgcggt
      841 tgaattgcag aagaagctca agaaacccgg ccctcccccg tccaagccga agttcaaacc
      901 ctgggatgag atcgtcacca aagaccacaa gaaggtcgag ggtctgctga cgctccacac
      961 caagggcgcc gaagtgcttc tcgaactcaa gaaggacgac ctcgacaagc ccatgctcgc
     1021 catcttgagc ctgtcgcagg gtatcgggtc gaacttcgtc tacggcggct tgcccatcga
     1081 cgacgtcatg ttcgacttcc accgcgacga ggacaacatc cagctgcgcc gcctgaccgc
     1141 gaacttccgc gccgagggcg acgaagagct gcagaaggcg atcgacctca ccttcgccga
     1201 atctatcctc gagagcttcc cgatcaagac cgagcgcgag ggcaaagtca tcatcgaggt
     1261 gaaggacttc ttcctctccg acgtcgccgg catgagcatt tggctcaacg gcgcgctcag
     1321 ccagcccatg cgcctggagg cgaagaaggg ctacttcggc agcatcaaga actacccgac
     1381 caacaccgag atcgacaccc ggctgaccta cgccgccagc cgtcccgagg cgctgttccg
     1441 tcccaacttc cccgatccgc gcgcgattca ggtcggcgtg gcgtggagca ttcgtaagct
     1501 gccggaagag ccgatgacgc cgcgcatcgc ggacgatcgc gtaggctact tcacgacttc
     1561 attcaaggat ttcaccaagg agaagaccga gaccttcttc acccactacg ccaaccgctg
     1621 gcgtttggag aagaaggacc cggccgcggc cgtctccgag cccaagcagc agatcgtgta
     1681 ttacatcgat cacacggtgc ccaaggagta cgtcccgtac atgatcgcgg gcgtcaacat
     1741 gtggcagaag gcgttcgagg cggccggctt caaggaagcc atcgtcggca agccggcgcc
     1801 caccaaggaa gaggacccca actacgaccc cgtcgacgcg cgctataaca ccatccgctg
     1861 gaacgtcagc gaccaggtgt cgtacggcgc catcggaccc tcgcgcgtcg acccgcgcac
     1921 gggcgagatc atcgacgccg acattctctt tgagcacaac atcgtgcaca acttcggcaa
     1981 gtcgtggcgc cgtgtggcgg ccccgcgtcc tgcgctgatg gaagtcgacc acaatttgaa
     2041 gcagttgtgg atgacgccgg aagagcgcga gcgcgaggaa gcgatcatgt cgctgccgtt
     2101 cttcaagggc cggccctaca tgctgtgctc ggtcaacgat tgcatgggcc tgggcgcgga
     2161 gatgatgcga ctcaccatgc tgatcaacgg tatgatcgaa gccggccagg aagttccgat
     2221 ggagtacatc ggcgccgcgc tgaccttcgt gtcggcgcac gaggtcgggc acaccatcgg
     2281 gctgcgtcac aatttcaaga gctccggtgc tgtgccctac gaccagctca acgacaaggc
     2341 gaagatcgac cagatcggca tgacgggttc cgtcatggat tacccgacgc cgaacatcgc
     2401 cgccaacgcg tcgcagcagg ggtactacta caccccgatc gtcggcacct acgacatgtg
     2461 ggccatcgag tgggcgtaca agcccatcga cggcgccact ccggaggagc agagcaagaa
     2521 cctcgaagcc atcgcccgca agtgcgtcga caaggcgtac ctgtacggca ccgacgagga
     2581 cacctatccg atgggtgcgc tcgatccgcg cagcaacacc aacgacctct ccgacgatcc
     2641 catgcgctgg gccaacgagc gtatgtcgat ctgcaacgac ctgctcatga agggtcagct
     2701 cgagggacgc gtcgtcgccg agggcggcga ctacgtgccc ttgcgcgcgg cggtgcaggc
     2761 gttgttcgtg cagaagtacg tgtgctcgaa catcgccacc aagacggtcg gaggcgccta
     2821 taccgagcgc gcccacaagg gcgacggcaa ggctcccttc accctcatca cggcggacga
     2881 gcagcgcaag gcgctccagt tcgtcgtcga caacgcgctc aagtcgaacg cgtacgcgct
     2941 gtcgcccgac atgctcaaca agatgcagga cgacaagatg tggagttggg agaacaacct
     3001 gttccagcag ggccgtcgtt tcgacttccc gttgtcggat tgggtcgaag cgatgcagac
     3061 gggtgtcctg ttcaacgtca tgaacccgtt cctgcaggcg cgcgtggtcg ataacgcgta
     3121 caagaataac agcgcattca agctgtcgga cttgtacagc acgctgacca agtcgatctg
     3181 gaccgacaat ctcacgccca acggtcgcac cgcgacgtgg gatcgcaacc tccagcgcgt
     3241 ctacaccgat atgctgatcc agcagatggt ggagccgtac ccggtgacgc cccaggatgc
     3301 ggtgtcgctg tcgcggctca acctgacgcg catcaagacc gcggcccagt cgggtctcca
     3361 gcgcaagggc ctggacgacg agaccaacgc acacttgatg gagacgatcg cccgcgtgga
     3421 ccgcgcgctg aacgcgagcc gcgaagcgaa cttctagttt ctccgcggac actggagccg
     3481 caagcgaaaa ctcccccggt cgattcgctc gaccggggga gtttcgtttt ggcgtgcaag
     3541 cgggcgcgtt atgccttgtg cttgcgaagc cccgggggca ggtcgttgcg gtgcgcgacg
     3601 gccttccaag gcccgtcgat gcacacgctc cattcccatg cccctttaac cttccgataa
     3661 aattgacccg cggtgtagta gcagtcgtcg tatccgctga cgacgtagag gtccatgcgc
     3721 tcgtcgtact cgaggatgac gctctggttg ccctgcttct tgcggtggcc gtgcgccggc
     3781 gcgtggtcgg gcgggccggg ctcgtccttg acgatgatca cgtcgcgcga cgaagggctc
     3841 ttggtcgcga ctttgcccga gcagccgatg acgacggcgg ccgtcatcag ggccagtgcg
     3901 atgatcatga gggtgcggat gacgaacata atcgacctcc attataagga gaccgatgga
     3961 gtactgagca agcggcgggc caggaaacga tggggaatcg cgagtcacgc cgacgcgcga
     4021 tgaggacggc ggcggcgcgg ctagttcttc gtcccgcgat agacgatcgc gcccgtgccg
     4081 accacgaacg cggtcgatcc cgacggggcc cagacgtcgg tgtagctccc gaaggtactc
     4141 gcctgcgagc gaatccaggc ctcgccgtcg tagttgaaga aggcgtcgta gaagttgttt
     4201 tggtgccggc cgcccaggcc cacgacgaag atgtcgctcg acgaaacgcc gaacaccgac
     4261 tccagcgcgg acacggtgct gatgctctcg ggctgccaag acgatccgtt gtagcgaaac
     4321 gcgctcgact gccccggcgc gcccgtgccc accgcgatga tgttcgacgc cgacgcgccc
     4381 cagatgccgc gcagcgcgat cgcgttgagg gtcgcgtgct cgctccagtt gccgccgtcg
     4441 tagttcaaca cgagtccgtc gttgccgacc gcgtacacgt tggaagcgga tacaccccac
     4501 accgcgtgca gcaccttcgt ggtcccgctg gtctgcgggt tccaggtgcc gcccgacaag
     4561 tgcagaacgg tcccgttgtc gcccacggcc cacgcttcgt tggccgcagc cgcccacacg
     4621 ccgttcagtc cttccgcggt tccgctcgtc tgcgtcgtcc acgccgcgcc gtcgtagccc
     4681 atgatgagcc cctggtcgcc gaccgcgatc acatgcgcgg gcgatgtccc cgatacgtcg
     4741 ttcaattgct gcgggcgtcc gccgaccatg aagcgccata ctccacccgc gcggtgcagc
     4801 acttgtgcaa acggccccac catgaagacg tcgttggccg acgtgcccca aatgccgcga
     4861 tagacggcgg tcgagccgac gtcgctcagt tccttcttcc actcgttgcc gtcgtagcga
     4921 acgatggtgc cgccggcacc gacggcgatc acgtcgtcgc ccgcggtccc cgtgatgccc
     4981 tcgaagttct ggctggtgaa aatggtgttg gtgaagcgcg tccacgtcga cccgttgtag
     5041 tggagcatga cgccggcttc gccgcacgcg aacacgtcgc tgggagtctg gccccacacg
     5101 ccgagcaggg cgcgcgaggt gattccggac ggctggctca tctgctgcca cgaacccccg
     5161 tcgtagtgca ggatgcgcgc ggtgttgccc accgcgaaca cgtcgtcctc ggcgaagccg
     5221 aatacggcgc gcaggttctc cgtggtgggc gtgggccggg gattccagga cgacccatcg
     5281 aagtgcagcg ccgtgcctcc gttgccgacc gccaccgcca ccgtatcgga cgccatccag
     5341 accccgcgga ggttctgtgt cgtgttggag ggccagtcga tcacggacgc gctctggatg
     5401 taacggatca cacccgcgtt gccgacggcg aacgcgctat tcgacctgac gccggagacc
     5461 gaattcaaac tctctgtcgt ccccgaagcc aaagacgcga agcccgtgcc ggtatcgcgc
     5521 agaatgaggc cgtcgcgtcc caccgcgaac acggtgccgt cggaggcgga tccgatcccg
     5581 aagatctccg cgttggttcc ggtcggatac gaggaccaca ggtttccgtc ccagtcgatc
     5641 acgagcccgt tgagtcccgc cgcgcgcgcg ccggtcggcg tcaccgtgac gccgtgcagc
     5701 gcgccgatct cggcgggctc ggtgacaaac gcgaactgct ccggcccggg cggatagacc
     5761 acgctgtcgt cgcacgcggc gagcacgccg cccagcgcgg ccgcggtcag ggctctcttg
     5821 caggtcgaag aaaacgtcat gtttgtcgaa gaggaaattc gggggacgac caaggatacg
     5881 cgcgatacga ggtcgtggtc aaggcggccc gtaacggagc acgagcccgc ccgacacgcc
     5941 gcctcccacg acgaacactt cggtttcgcc cgaatgggcc gcgtacagcg gttgcagcac
     6001 cggcgagtcg tcgctcgacc aggtgccgtc tcggtaacgc agcatcgacg cgccgccccc
     6061 gaacgcgaga atgtcgccgg cggggtcgcg ggcgagcgca tataagttgg cggacgtggg
     6121 agactcgaga gccgtcacgc cctgcgtatt cagtacgaag agcgcccccg actcgccgat
     6181 cgcgagcgcg cgacgattga gatcgtcgcc cggggcctcg atgacgtcgc ggatatcgac
     6241 caacgagcgc gagttcagct gcgtccaccc gatgccgtcg taccgaagca cgacaccgcg
     6301 gtcgccgacc gcgtacgcct cgctgccgga cagacacgcg accgcgttga ggctctcggt
     6361 gaagcgcggc atgatctcgg tagtccagcc cccgcccgag cgccgtcgca gcgttccgtt
     6421 cgatcccacc gcatacaact cggtcgggct ttcggcgaag ccgtaccacg tgatgatgtc
     6481 gtcgatctcg accacccact cggtgccgtc gaagcgaagc aggcgctgca cgccgccggc
     6541 gaacagctcg ccgcccgacg tcagatagac gcagtacaaa tcacgcgcgt cgacgccttc
     6601 gtcgacttcc caaccatcgc cgcgtccgcg cgcgatgtag ccatcgtccc cgacgacgat
     6661 ggcggacccg gatggatccg cgcccacgcc gcgcaggatc ggcaccgctc cggtcgcttc
     6721 ggaacgccac gccgtgccgt cgtggacgcg aacgacgcgc gagccgccca cccacgtgtg
     6781 ggtgccggca cggcaggcgg cgtagagcca gtgcgtggtg cccacctcga cgcgcgacca
     6841 ggcgccgtct tggagcagca gtgcgacgcc gttggcgccc acggcaatcg ggtcgcccgc
     6901 gcccgcgacc acgcactgca gcacgctgcc ggtcgggctc gtcatggggg accagtccgc
     6961 gccgtcgtag tgcagaatcg tccccgacgt ccccaccgcg tagacgtcgt tggacgcagt
     7021 accgctgacc gacgccagtg tctcgagcgt tccgctggat tgctcgctcc atgcgtttcc
     7081 gtcgaagtga atgatgagcc cgtccggacc gaccacgaac acgtcgcgca tcgacgtgcc
     7141 ccagaccgac agcatcgagg cccgaaccgg aggcggcgcg agcgaccagg tcgaaccgtt
     7201 gccaacgagc aaggtgccga gctcgcccac cgcgagcagc gtgtcgtccg acgccgccca
     7261 caccgagcga agatcctcgg ccgtaacctg gggcgttggc gcgaacgcga tcccgttgaa
     7321 ggaataggtc gcgccgccgt caccgaccgc aaccaccgac ccatcgggcg ttccggtcac
     7381 gctccacacg ttacggaaca gatcgaccgg gacgctcgtc cagcgtacgc cgttccagcg
     7441 cgcggcggtc cccgcgggtc cgaccgcgaa cacgttgttg ggaccgaccg cccacatcga
     7501 gtagacggtg aacgagggcg ggagctcgcg ccactcggac cactgcggtt cgcttgcctc
     7561 gggaggagaa ggggcaatgg gatcgtcgtc gcacgcgaga aggcacgcga gcgcgacggt
     7621 caacgggatc gcgattgagt aacggggacg catcgattcg ctcaaccgag cacggggcgt
     7681 cgtggtggga tgcatcaatt gtatgccgcg gccccggccg gcgcaagcct agatcgggta
     7741 cgtgaacccc gagaggcgct cgctttcctt ccagagccgg cgctggatct cgcggtcgtt
     7801 cgattgcgcc gacgtgccca tcggcttgca gtggcgaaaa tacttcccgc tgacgccctc
     7861 cacctccggc gacgatgcca ggtagaccgg cgtttcggcc ccgtcctcgg gctgcttgaa
     7921 ccagcgcccg accgcgtgcc acgcgagctg catcaccgac gagaagttgc tcaggagatt
     7981 ggtgtggatc acccccgggt ggagaacatt cgcggtgacg cgtgtgccct ccagccggcg
     8041 cgccagctcg taggtgaaga ggatgttcat gagcttggtg ttcgcgtagg cctgccagcc
     8101 gcggtacgcc tccgcgagct gcaagtcgtc gaagtcgatc acaccccgcg agtgcgcggt
     8161 ggatgccacg ttgacaatgc gcgccggcgc gctcgcgcgc accacatcga gcaacaaccc
     8221 ggtcaggaga aaatacgcga ggtggttgac gaagaactgc atctcgaagc cctcgctcgt
     8281 gagtttgcgg cgcgacgtga gcacggccgc gttgttgacc aggacgtcga ggcgcgggca
     8341 cttgtgcgcg accacgtccg cgagccgccg aatggcggcc gtggtggaca ggtccgccac
     8401 cacgacctcc agcgaggcgc cgggcatcgt gcgcagaatc tccgcgcgcg ccctcaagcc
     8461 ccgctcctgg gtgcggctca ccatgacgac gcgcgcaccc agcgcggaga gcccggccgc
     8521 ggtcgcctta ccgatgccgg agctcgcgcc cgtgaccagc accgtctttc cgtccatgcg
     8581 gtccacgcgc gcatgatacg ctagccggca tgagcgaagc gacggcgaaa gcacgcgcca
     8641 tcatccacgt cgacatggac gctttctacg cgtcggtcga agtgatggat gatcccgcgc
     8701 tggcgggcag acccgtgatc gtgggcggaa cgcgcgaatc gcgcggcgtg gtgtcggcgg
     8761 cctcgtacca ggcgcgtgtc ttcggcgtgc gcagtgccat gagcaccgcg cgggcggagc
     8821 ggctgtgccc gcacggcgtg ttcatcgccc cgcgcatgga tcgctacgcc gagatatcgc
     8881 acgacattcg gttgatcttg gacgcgtaca cgccgctcgt cgagcccatc tccatcgacg
     8941 aagccttcct cgacgtcacc gggagccagg cgctctttgg aaccgggccc gaaatcggcc
     9001 gcgcgatcaa gcgccgaatc cgcgacgaga tcgggctcgt cgcgtcggtg ggggtggcgc
     9061 cgaacaagta cctcgccaaa ctcgcgagcg acctcgagaa gcccgacggc ttcgtcgtga
     9121 tcacccaaga ggaagcggaa gcacgcttgg cgccgctgcc ggtgtggcgc ttgtggggtg
     9181 tcgggaagaa gacggaagaa gcgtttgcga aggcaggatt acgcacgatc gccgatgtaa
     9241 cgcgagcgcc ggacgcgctg ctggaacaac tgacggggtc ttacgcgccg cgcctcaaac
     9301 agctcgcgcg cggcatcgac gatcggccgg tggtccccga cgccgacgcg aagtcgatcg
     9361 gggcggagaa taccttcgcg cgggacatcg ccgacggggg agcgctgcgt gcggagttgg
     9421 acgcgctatc cgagcgcgtg gcgcgacggg cgcgcgcgga cgacgtcgtg ggcttcacgg
     9481 tcaatctcaa ggcacgctac gccgacttca ctaccgtgac gcgcgccaag acactacccg
     9541 acgcgacagc ggactcggtg gtgattcgcg acgtggcgcg tgacctgttg gagaagaagc
     9601 tcgaccgcca aggtcgcgcg ctgcgattgc tcggggtttc gctgtccaac ctggcgcgcg
     9661 cggaggaaac atcgctcgat ctgttcgaat cgaacttgcg cgcgccggcc acgcgcaatc
     9721 gcacgctcga cgcggtgatg gactcgctgc gcgcgcgctt cggatcgagt gcggtgaggc
     9781 ggggaacgaa acggggcccg cgctagcgcg cggaccccct ttcgtgagtc accaaaagac
     9841 taattgatcg tcccgaagag gccggtcggg ccgttcggag caccggatgc accgagtccg
     9901 ccggtaccgc ctgcgccgcc gctgaacgtg ttcccgatcg tgaccggatt cgacgcaccc
     9961 acacggtaga tgccgtagct cgggccaccc ggtccgcccg cgcccccgcc gccctggcca
    10021 ccgtcgccgc ccgccccgcc gtttcctccg cgcccaactt cgcccaggca cgccgtcgct
    10081 cccgatccgc cgaaaccgcc gttgccaccc gatccgccga aaccgccgcc gccacccgtt
    10141 cccccgatgc cgcgcaggaa tgtgcatcca tcgaatgtgg gcgaggaatt cacgagcatg
    10201 acgcagaatg ccgcgcgacc gcccacaccg ccggtgccac cggtgccgcc ggaaccaccg
    10261 ccgccaccgc caccaccgcc gtttccgccg ccgtcgttgc agaacgtgcc accctgaccg
    10321 cccccccctc ctccgcctcc tcctccgtca ccgggcgcgc ccacgaggcc ggagcccgac
    10381 gtgttcggaa cccactcgtt ggcgatgatg gacccgttcg gcgacgcggc cgcgccgtcg
    10441 gtccccgaaa tgccaacggc gcccgtgctt ccattccctc ccgggccccc cactgcatcg
    10501 caagtcagac caccgcttcc tgcatctccc tggccgccga cgccaccggc gccacccgta
    10561 cacgcacccg cgattccggg gaagccatca ttcggtccct ccaccccgcc ggcccctccg
    10621 gctccccctg gacatccgcc gggcgacgct cccccagctc ctcccaagcc ccgcgcgccg
    10681 tcgcagcttc ccgcctggcc ggtgaaaccc gaactggcgt tcgctccggt cgtgccgtta
    10741 gcacccgaat tgccgctaag cgcgccatcc acatcgaacc ggcagtcctg gaactgaagg
    10801 tctgcgtccg acccgaccga gtagagcgcg atggagttcg ttccggtcgc gggctgggac
    10861 atgtaaagcg agacgcggcg aataagcgtc ggcgtggtga tgttattcgc aagcgcgcga
    10921 ttgagggtga agttgaacgt ggaatatccc gacgggctct cgtcccattg cggggtgcgc
    10981 ccgcccatga cgtcgatgcc cggcaagaag gcgggcacct gtccgttgta ggtgccttcc
    11041 cgagccagga tcaccgtctt tgcggtttgt tgggcgacct ggtaagccct cgccagcgtc
    11101 aggacgggag tggcgatagt tccgtcgttg ccgtcggaac cggagggcga catgtaaaca
    11161 acggtgggat cgttgggcac gatgtcgagt tcgatgacgt cgaggccgat gcctccgtcg
    11221 tcgtccgtca cgcgcagttt cgcgcgatac gcgccgggct ccgggtaaac gtggctggtg
    11281 ctggccgtcg tcaggctcga atagtcgaac gtaccgtcgt cctcgaagtc ccactcgtac
    11341 cgagcgatgg tcccgtcggc gtcgcttcct gagccggtga agttcacggg gatgtcctcg
    11401 aagagcggcg ttgcgttccc gacgttcgcg accggcggga tgttcgaatt gatccgcagc
    11461 agcatgcccg ccagcgagac gttttctccc gccgtggtga tagtgccgct ctcggtgtct
    11521 tccaggtaac ccgcgtgctc cgcgcgcacg gtgtagctcc cgaccggcac attggtgatc
    11581 gcgtagtcgc cggttgggtc ggtgacggcg acgtaggacg ttccttcgac gtaaacgacg
    11641 gtaccttgat ggatggccgc gttctcgagc gcggccacac cggagaaggt cccggtcggc
    11701 gtcaggttca cgtcgacgta gaccgtgtcg acgaaggcca ttcgatcggg gacgcggaca
    11761 ctctgcatgc cgcccaggtg atcgtcggcg atgacctgca ggtagtactc gcccgcctcg
    11821 acgtcttcga aggcgaagcg gccgtggtcg ttggtggtgg tgacgcgacg gcccggcgtg
    11881 gacgcgctcg cggcgtccgg attcttcgac aggaagtccg cagacgcggg gataccctcg
    11941 ttggcgggct cgatgacgac gaccgcgtcg gcgacggcac ttcggtcctg gtcgcgcacg
    12001 aggcccgcga cgacggtcgc ttgcggtgtt tcgccgttac ccagcggagc gtagtcgtcg
    12061 ccgtcctcgc cgcacgcgag aaagatgagc gatgcggcga gcagcgcgcg ccgggtgttg
    12121 aaatggagcg agaagcgtcg ggggccgtgc atgggtaacc tcgcggttga ttccgggttc
    12181 aacgaagacg aacgaagcgc cggacgatct cgtttccgcc cgcgcgcagt ctgatcaaat
    12241 agattcccga cgcgacggga gcgccgctcg cgtcgcgcaa gtcccagcga atggtgcgag
    12301 tgctcgcggg cccgtcgccc tgccatccca tgaccagacg accgcgcacg tcgtagacct
    12361 cgagccgcgt ttccacaccg tcgggcgcgt cgaggcggaa cgtcacttcg ttggtggcgg
    12421 ggttcggcca gggctggtcg aggaaaaggg gagcgcgcgt ggtgacgtcg accccggtgg
    12481 cgtcttcctc gacgacgata ccgcccacca ccgcggagag gtaattgtcg acgcgcacct
    12541 ggatggtgta cgtgcccgcc ggaatgttat cgatgcagta gcggccgtcg acgtcggttc
    12601 cggcgtacag gccggtgtac tggtcgagat tatcgaaaag gaagacggcg gcgttgggga
    12661 ctggctgcag ggtcacgccg tcgcggaccg taccgcagat ggaacccgcg cggcacgggg
    12721 cggcgcccag gaagaggagc agggccagca cacctgcggg ggccatcgaa gcgcgaagcg
    12781 cgcgtcgact cacggtgcga cctcagtggt tcgggtacac tgctgagtaa acagtgtatc
    12841 agagggggcg gaggaacttc cagcgggaat tcgcggggtc agcggtggta gcgaaccacg
    12901 ccaccctcgg tggcggcgat gatctcgcgc ccgggggcgc cccaaaccga ccgcacgggt
    12961 cgcgtcgtca cgcgcggcaa gtacgaccag ttgctcccgt cgaagtggag cacgtcgccg
    13021 cgcccctgtc ccaggaacac gtcgtgctcc gaggttcccc agaccgagta gttggagcct
    13081 cgtgtgtagc cgatgaagat cgcctgccat acgcggccgt cgaaccggca gatctcggtc
    13141 gagttcgagc ccaccgcgaa gatgctgttg gggccgaagc cgaacatgtc gaggatgtgc
    13201 cagcccaccg gcgatggaaa cggtctccac tcgcggccgt cgtatcgcat cagggctccg
    13261 ttttgacccc cggcgaacgc gccgtccttc cacgcgtaca cggcgaacaa gtgatagctc
    13321 gtccccgact ccatcggcgc ccacgaatcg ccgtcgtgcc ggacaatcgc gccgttgtag
    13381 ccgacggcga agatctcccg gtcgttgccg gcgaccgagt tgagctcctt gcccgcgccg
    13441 ccgctgaccg cggtccacga cgcgccgtcg tactcgagaa tcatcgacgg acccacggcg
    13501 tatatctcgc ggccgcttcg gccccaaacc gaatacgccc gggccgaagt caagtcttgc
    13561 ttcttcaagt cggtccacgt gccttcgtga taacggtagg cgcgcgttcc gaccacgtag
    13621 atgccgtctt cctcggagcc ccagaggtcc atgaagtcgt agccgaatag ctccgggtca
    13681 ccgagcgacg gagtcgcctc gccgcccgca atcttccaga cgacgtcgcc cgccgcatag
    13741 agcgcgttgc tcgactggtc gccccacacg acgcgcacgt agcggtcgat gtcggccgcg
    13801 cgcgaccacg cgcttccgtc gaaacgaagg agcgacgtgg tcggatagtc gaactcggcg
    13861 gcgcaataca cttcgtcggc ggagcgaccg aagatgcgag gcgaccacag cgacattccg
    13921 agatcgacgg gagtcatctc ggtaccgtcg tacgtgaaca aggagtcgta cgtggccaga
    13981 tacagcggac catcgggagc cttccatatc gattgatagt agtgctgggc tcgggggcgt
    14041 gacgcgttcc agcccagtcc gtcgaaatgg ccgatccagc cggcgtagcc ggcgatgtag
    14101 acgtcgtcgg gactcgcgcc cgacaccaaa tgccacgggt ccccccggtc gtcgaccttg
    14161 aggctgtcga cgaaccaatc cgaaccgtcg tagtgataga ggcggtggtt tcccccgccg
    14221 accacgaaga tttcgccgtt gtcggcgccc cacacgtcgc cgatttgcgc ggggcgcgcg
    14281 atccggtgcc actgcgcacc gtcgaaccgg tgcatgtcgg agtagcccat cgcatagagg
    14341 tcgccgctcg aggtgcccca ggtcgtgagc agtccgtcgt ccgcgttcgg aagcgcgatg
    14401 acctcccacg tatccccgtc gaggcgcatg atcgagctcc ccgacgcgta cacgtcgccc
    14461 gcggggccgc cccacaaact gacgatcggc gagtcgaagt agtccatcga ggcctggtag
    14521 ggagtcgtgc tcgatccgcc gtggcccggc ccgaccggat cgtccgagca cgaaacgagc
    14581 agcagggccg cggcgagagc ggccgaggaa tggcggaaat gtgtcacggt aatcgccgga
    14641 atcataacgg attccgggtt tgggaaccag tggcgcgcgg tgcggtctag cccgcgctct
    14701 tttcccgctc gtcgaacgcc ttcgtctcgc gctcgagatc gcgggtggcg gactccaagt
    14761 gctcgtaggc cgcgtcgatc ttgggcccga cctcgcgact cttcttcgac agctccgcga
    14821 tcgcgctcgc gttgcccgtc gccgacgcct cgctcaagag tcgaaaggtc tcatcgcgct
    14881 cgcgctcgag ggactcgata cgggcttcga tctcggccac gcgcttctcg agcggcgcca
    14941 gcttgcgcga gcgctcctgg atcatcttcg cgcgcgcttg gcggttcgcc ttgcgatcga
    15001 tcggcgcggc cggcggcgga ggaggggccg gtttggcagc cggtgcgggg gcgagtttcg
    15061 ccgccgtgct cggcttggtg agcttctcgt ccatctccca gccgacggta tccaggaagt
    15121 cctggtagct cccgtcgaac acgcgcacgc gcccgcggtc gaacacgacg aaatgcgtcg
    15181 ccagcgtgtg caggaacatt tcgttgtggg tgacgatcac agccgcgccg tcgaaggcgt
    15241 cgatggcggc caacagcgaa tcgcacgagt ccatgtcaag gtggttggtg ggctcgtcga
    15301 gcaacaacaa gttgcacggc gtcacgatca gcttgcccaa agtgacgcgg ctgcgctcgc
    15361 cgccggagag caccgcgatc ttcttgagcg cgttgtcgcc ctcgaacatc atcgcgcccg
    15421 cgatgtcgcg cgcgatttgc ggagacgtca tgggcaccgt ggattgaatc tcttcggcca
    15481 cggtgaggcg ttcgtccagc cgggccacgt tggtctgcgc gaagtagccg gacacggtgc
    15541 gcgggtggct ggtgatggca cccgagcgcg gcgcgagctc ccccgccagc atgcgcagca
    15601 gggtcgactt gccgcgtccg ttgggcccga tgacgcagat gcggtcgcgg tcgtggatct
    15661 cgaagcgcaa gtccgagatg agctcgggtg cgtcgccgta gccgaacgac acgtggtcga
    15721 ccttcatcat caccttggcc gggaacgctt gggcccggaa cgaaaactcg agggtctgca
    15781 aatgctcgag cttgtcgagc cgctcgtgct tggccagcat cttgatgcgc gattgcacgc
    15841 gcgtcgccat cgtgttcttg gcccgaaagc gctcgatgaa gcgctcgatg tccttgcgct
    15901 tgcggtcctc gttcatgcgc gtcttctcgt agacttcttc ctcctgcagg atctgcgcgt
    15961 acagcttgcc ggtatccccg gcgatcttct tcgccttgcg gcggtgaatc gcgaccgtgt
    16021 gcgtgaccac cttgtccatg aacgagcggt cgtgcgtgat caacacgagc tccgacttcc
    16081 attcgttcag gaagcgctcg agccagcgga tcgagaccac atcgaggtag ttggtgggct
    16141 cgtcgaggag caggaggtcg ggttcggaca cgagcacctt cgcgagattc aggcgtactt
    16201 ggaagccgcc cgagaacagc tcgggcgcgc gcgcgaggtc gtcttcgccg aaacccaggc
    16261 cgaacaggat ctgctcggcg cgccaaagat cgtcgctgtg atcgggcggg aggccgaggg
    16321 ccgcttcgtc gagcagggtg ggctgggtga agcgaatgtg ctggtcgagg tgcccgatgc
    16381 ggtatcgctt ggggaccacg atgtcgcccg agtccggcgt cacgtcgccg agcagcatgc
    16441 gaaacacggt ggtcttgccg tggccgttgc ggcccaccaa gcccacgcgt tcacgacgat
    16501 tgaccgcgaa cgacaggtcg tcgaagaggc gctgggcgcc gaaggacttg gtgaccttgt
    16561 tgaactggat cacggcgagg aaggccgttc gagcctcatc aagaccgcgc cggccgcccg
    16621 gcgctcgacc caatcggcca tgacgtaacc gttctgcttg attcgaacca tcatgtccgc
    16681 gctctcagag ccgtggacgt gcgagaagac gatccaaaac tcgcgcggtt gatcgacgat
    16741 ggtctgagaa agcgatttga tcttcgcctc gggagtcggc agcgagcgca gccagcgacc
    16801 atagtagatg tttccgctcc ggcagaaatc cggcggcgta tcgtcgtgcc agcacgcgag
    16861 gttccacgtg ggcggcagat caccgcgcgt gtccggatag cgctgcaggt agtacgcgaa
    16921 cgcgggtgcc gccccgtaga aaacgtacgt cggctgcgac cgcgaccact tgtcgtgcca
    16981 gtactcgacc accggccgca tgtcctcggt ttcgggccac tgcacggtgc gctgcgggtc
    17041 gatcgcatcg cgcacggtcc ggttcgggag cgacaccaga ctgcacgcga tcaggcccgt
    17101 gaacgcgagc gcaccgaaga gcttcaacgc gcgatcgcgc gcccccatcg tgaacccgat
    17161 ggaaacgagc acgagcgcgg gcggacacag gatcagcccc cagcggaaac cgaaggggaa
    17221 aatctcgagc aggcctgcga tgccgtacgc cgcccacgcc gccgcgaacc agagcgtcca
    17281 gcgcgggcgc gcgcgcatcg cgagtccgag caacgcgagc gcggacacga ccgggatcca
    17341 ggtgggtacg cgcgagaacg gaaagcccgt gaagaagaag ccgaacagat tcgcgagcca
    17401 tcgccacacc agcgcgatcc cttccgggcg ctgctgcgga tactcggcga gtgccgcgcg
    17461 cgtgttgagg acgcgctcga gctggtcggg caggacggcc cacaacacgg gcgcggtcac
    17521 gagcgcgtag attcccagcg cgagtgcgag tcgccgtgat cgagcgcggt cgcgccgtac
    17581 cagcgcttcg accagcgcgc agccgcacgg caccagcacg cagaacacgg acgcgtagta
    17641 ggcggtggtg gcgagaaagg cgaccgcggc ccagccgatc acccaccggc gtccggtttc
    17701 gttccacaac ccgtgcagtg caaccagact ccacgacacc gttcccatca tgagcgcgta
    17761 ctgaccgact tcctgcgcgt agcgaatgtc gggcgggttg atcgcggcca agacgccggc
    17821 cgcgaccgcg gccggaacgc cgcccagacg ccggccaagc accatcacgc ccgcgacacc
    17881 ggccaggctc gcgatcaccg acagcaatcg cagccacgac tcgttggtcc cgaccttcat
    17941 ccagaaatgg agcaggaagg aaaacaacgg cgggtcgccc gagcccacgc tgacgaagtg
    18001 cgcgagttgc gatagcggtg cggtcgccac ccagtactcc gacgcttcgt cgaaccacag
    18061 ggaacgcgcg ccgaggtccc acagtcgcag cgcgagagcg aggacgagga tcgcggcgaa
    18121 ccacatgcgt tcgcgcgggg acgcggacaa tcgaggaatg ggacgcatga accggctagt
    18181 cgctcgcggt ctggatgcgg cgcaccacgg cggtcaccat gcggcgcggg aagaaacgga
    18241 ccgtgaacgc catcacgttg ttcagagcgc ccggcacgac caccgccttg cctttcatca
    18301 tgcccgcgta acccgcgcgc acgaccacgt ccggattgat gcgcatcatg agtcgcagtc
    18361 cgcgcacctt gcccacatgg gcgcgcttct ggaactcggt gggcgtgggg cccgggcaca
    18421 acgcggtcgc ggtgacgccg gtaccacgca gttcgttcgc gatcgcctcc gtgaacgaga
    18481 gcacaaaggc cttgctggcg tagtacaccg actgcagcgg cccgggctgg aagcccgccg
    18541 tcgacgcgac gttgagaatg cgcccgcgct tgcgcgccac catgtcgcgc gcgaacaact
    18601 tggtcaggtg gacgacggcg gcgatgttga cctgaatcat gtcgagctcg gtctgcaaat
    18661 cggactggtg gaacaagccg tacatcccga agccggcgtt gttgacgagc acgtcgatct
    18721 cgagtccggc cgcgcgcacg tcgtcgtaga tcgcgaccgg ggcgcgcggg tcggcgaggt
    18781 cgtgaaccac gacgcgcacg cctacgccgt gcagcccctc gatctccttc ttgagcgcgc
    18841 cgaggcggtc ggcgctgcgc gcggtgacga cgaggtcgta gccctcggag gccaagaggc
    18901 gcgcgaactc ggcgccgagt cccatcgacg ctcccgtgac gagtgcgacc ggtcgcgcgg
    18961 cggccactac gactccagcg cctgcctcaa gtcgtcgatg aggtcgtcgg tggcttcgat
    19021 gccgaccgac aagcgcacca gcccgtcgcc gatgccgatg cgctcgcgct cttcgggcga
    19081 cagcagccgg tgcgaggtct tggccgggat ggtcatgagc gagtcgacgc cccccaggct
    19141 gggcgcgatc tcgggcagct ctacgcgctc gagcagccgg tcggcggcgc gcgcgcctcc
    19201 cttcagatga aagctcagca tgccgccgaa gccgtcgaag aactcggccg cgcgcgcgtg
    19261 atgcaggttc ccaatgagcc cgggatagtt gacgctcgcg acctccgggt gctcgtcgag
    19321 gaagcgcgcg atcgccagcg cgctcacgtt ctggtggcgc acgcgcagcg ccagcgtctt
    19381 cacaccgcgg tgcaggaggt agcacgcgtg cgggtcgagc gaaccgccca ggtgattcag
    19441 gttcaagcgc acctgctcga ccaaatccgc gcggccgatc accgcgcccg ctacgatgtc
    19501 gttgtgaccg ttcatgtatt tcgtgcagct gtgcagcgac agg
//
LOCUS       DASPGK010000444         7456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_695318, whole genome shotgun sequence.
ACCESSION   DASPGK010000444 DASPGK010000000
VERSION     DASPGK010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695318"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1602)
                     /locus_tag="VEC56_12485"
     CDS             complement(73..1602)
                     /locus_tag="VEC56_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV15015.1"
                     /translation="MDPKRLGGYELRDVLGEGGMGTVYRAHDPTLDRPAAVKVIRAKA
                     LSNEGKERFLREARASSKINHPNIITIYAAGEEDGAPYMAMELIEGKTLREVIDEGGI
                     DWRVSTRWIVQLLDALQRLHAEGIVHRDLKPENVMVTKEGNVKLMDFGLAHLASTTAL
                     TQEGTTLGTVPYMSPEQVLGRKLDARSDLFSVATIYHEMLTGQHPFRGEHPMAVMYSI
                     RNETPKPLKLQSSDCPIELQSVLDHAFQKEVDKRYADATAFRNAILEVVPELSGAAPT
                     ESRTSPVRMALIIGGISVAVFGFAVSAWNVAQKKTREAARAEAANLNESGGEFELKQD
                     YGSARLKYREAIERDPTYAPPYNNLGVLSEQDGDFEIADDLYRKAVASDPAYAPALLN
                     IGNRFLAQQQKDSAEVYFRRAARGNDVDAGAHQPGALAAANQLGFLLWERGEYHEALA
                     VIDTALAREPAPVVKAYLLKNKGKNVAALGDSTAARVLWAEAAALNPNDDELRSLLAM
                     P"
     gene            complement(1687..2628)
                     /locus_tag="VEC56_12490"
     CDS             complement(1687..2628)
                     /locus_tag="VEC56_12490"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HXV15016.1"
                     /translation="MLKRWIPALVLGLATVLVVTHSPGPAPAQNDSPTAVLTSCKGPV
                     TIVRAGNGPVAATFGAALSEGDEVKTGADAEAEIMFASGNWVQVGANSSMKIKGQPGA
                     KPKEQNFEVVQNFLKLKSTEGTSSISGLRSGDKKSDLRAVSPCQTKVRDARPTFTWTA
                     EDPSLELQFVVYNETGIHWQSTVSGATSVAYPADAPSLVPGVSYSWTLETSDPLVSPP
                     LRTAASFFEIIAPNDVTALDQDLASIEAKKPGATSYHLMRASLFFDRGLVADAIAETQ
                     AAVAADPDNASLHAILGRLYAETGRTDEAMKEMEKSR"
     gene            2849..3250
                     /locus_tag="VEC56_12495"
     CDS             2849..3250
                     /locus_tag="VEC56_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15017.1"
                     /translation="MKKVGIWIDHRRAVVVTIENGSESIKTIEGDVDRQPKAAGRSGN
                     ATKWGPQAPINERRIEENYRLHVVHFYKDVIKSIGKPDQLLVMGPAQAKLEFAEEIEK
                     VAELRGVPLKVETTDKMTDPQVAAKLRATEF"
     gene            3299..3754
                     /locus_tag="VEC56_12500"
     CDS             3299..3754
                     /locus_tag="VEC56_12500"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV15018.1"
                     /translation="MKRLYGMVALLLMLASSAFAMSVEERTKLENEVKAAENAFAKTL
                     ADRDHKAFARFIADDAAFFGRNSVSRGKEAVVASWAGLFEGPTPPFSWASEAAVVLES
                     GTLALSYGPVYAADGKRFGTFNSVWRREKDGTWKVIFDKGCDYCEAGTK"
     gene            complement(3758..4822)
                     /locus_tag="VEC56_12505"
     CDS             complement(3758..4822)
                     /locus_tag="VEC56_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXV15019.1"
                     /translation="MPPTPPPIHVGRIKTGLKLFLAFSVIGFIIVFSRSSVGDTIRSL
                     THLDPRWLAAAVGLVVFDWLVSALRIYIFANRIHPAITYGACMRSCLANVFLGGATPS
                     QSGGAAAQIYVLWAEGMTVLDATVACFLGGFLGTVIVLLGCAVSFSIFAQPDFLGSNL
                     RVISGASLGLFALILVIVLFSLVFPGAFKRAVHGLLRSLPWARRKLESRHAVERLFDT
                     VDRYHELMRRFMVRGKTLFAFGLVLSAIIYFNKFVIAFVILRGLGGTVDLVHVLYMQV
                     VLLLIFYFSPSPGASGFAEVTTIAVMGSVIPKGSEAAFVLLWRFFTLVLNMMIGAGVL
                     VSYLSGKRGRRRSRDDAQAA"
     gene            4912..5304
                     /locus_tag="VEC56_12510"
     CDS             4912..5304
                     /locus_tag="VEC56_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV15020.1"
                     /translation="MNTNQLKSALSTIGQIAVTVRDIPRAVTFYRDVLRVPFLFEAPG
                     MAFFQAGDVRLMLGLPEHEAQRFSSILYFKVDDINGVYGDLKERGVPFTHEPRLIHRG
                     SQTDLWLAFFSDPDGNTMALMSEVARRD"
     gene            5390..5944
                     /locus_tag="VEC56_12515"
     CDS             5390..5944
                     /locus_tag="VEC56_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV15021.1"
                     /translation="MRAVLVAAVIVVCGVAIHAAEDPATTEKRVQRGKYLADTMGCQD
                     CHSPKIFSPEGIPHPDESKAYSGHPGSKLPAVDPKSLAPGNWYLMSPDLTAYAGPWGI
                     SYAVNITPDEQTGIGLWTEEIFLQAIRTGKHMGNGRPILPPMPWPYYRNLSDEDLKSL
                     FAYLKSLPPVKNAVPAPVAAADLQ"
     gene            6100..>7456
                     /gene="miaB"
                     /locus_tag="VEC56_12520"
     CDS             6100..>7456
                     /gene="miaB"
                     /locus_tag="VEC56_12520"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXV15022.1"
                     /translation="MNLNVYIETYGCQMNVYDSELVKSILVTRGHRLVDRFDGADVVL
                     INTCAIREKAHQKIYGRLQQLKRERALRRSELTIGILGCMAQNLKQDLVDGHDEVDFV
                     AGPDSYRHLPELIDAARGSAEATPALAGAALKLSTTETYTGVDPVRSGGINAWIAVMR
                     GCDNMCTFCVVPYTRGRERSRDPEGVIDEVRALVRDGYKQVTLLGQNVNSYRFGEVRF
                     ADLMRRVADVPGVERVRFISPHPKDFPVELIRAIAEHPKLCKHIHLPLQAGSDRVLEL
                     MRRTYTNAEFRALVDEMRGTIANLSLTTDVIVGFPTETAAEFDETMRVIDDVRFDAAF
                     TFKYSERKGTYAQRKLRDDVAAEEKTKRIVALVDLQKRITDEVNRGLIGSTHDVLVEE
                     HDEKRPGFVVGKTDHFKHTILPADGAEIGDTVRVVVENAHGATLYGRALSTHARPARR
                     AV"
BASE COUNT         1360 a         2397 c         2427 g         1272 t
ORIGIN      
        1 tccggctgct tcgagcagcc ccagaacgac gcgacggcga cgatcagtac aacgacgatg
       61 gcacgcatcg gactacggca tggccaagag cgatcgcagc tcgtcgtcgt tgggattgag
      121 cgccgcagcc tccgcccaga gcacccgcgc cgccgtcgaa tcgcccagcg cggcgacgtt
      181 cttgcccttg ttcttgagga ggtacgcctt cacgaccggc gcaggctcgc gcgccagtgc
      241 ggtgtcgatg acagccagcg cctcgtgata ctcgccgcgt tcccacagca ggaaccccaa
      301 ctgattcgcc gccgccagcg cgcccggctg gtgcgcaccc gcgtcgacgt cgttcccgcg
      361 cgcggcgcgc cggaagtata cttccgcgct atccttctgc tgctgcgcga ggaagcggtt
      421 cccgatgttg aggagcgcgg gcgcgtaggc gggatcgctc gccacggcct tgcgatagag
      481 gtcgtccgcg atctcgaaat ccccgtcctg ctccgacaag acgccgagat tgttgtacgg
      541 cggcgcgtaa gtcgggtctc tctcgatggc ctcacggtac ttgagcctgg cactgccgta
      601 atcctgcttg agctcgaact cgccgcccga ctcgttgagg ttcgcggctt ccgcgcgcgc
      661 ggcctcgcgc gtcttcttct gcgcgacgtt ccacgcgctc accgcaaagc cgaacaccgc
      721 cacggatatt ccgccgatga tgagcgccat gcgcacgggc gacgtgcgcg actcggtggg
      781 ggccgcgccg ctcagctccg gcactacttc gaggatggcg ttgcggaacg cggtcgcgtc
      841 cgcgtagcgc ttgtcgactt ctttctggaa ggcgtggtcg agaacgctct gcaactcgat
      901 cgggcagtcg ctggattgga gcttcaacgg cttcggcgtc tcgttgcgaa tcgagtacat
      961 caccgccatc gggtgctcgc cgcggaacgg gtgctggccg gtcagcatct cgtggtagat
     1021 ggttgcgacc gagaagaggt cgctgcgcgc gtcgagtttg cggcccagga cctgctcggg
     1081 cgacatataa ggaacggtgc cgagggtggt gccttcctgg gtgagtgcgg tggtggaggc
     1141 caggtgcgcc aggccgaagt ccatcagctt gacgttgccc tccttcgtta ccatcacgtt
     1201 ctcgggcttc aagtcgcggt ggacgatgcc ctcggcgtgc aggcgctgga gcgcgtcgag
     1261 caactgcacg atccagcgcg tggagacgcg ccaatcgatg ccgccctcgt cgatcacctc
     1321 gcgcagcgtt ttgccctcga tcagctccat cgccatgtag ggcgcgccgt cctcttcgcc
     1381 ggccgcgtag atcgtgatga tgttgggatg gttgatcttc gaggaggcgc gcgcttcgcg
     1441 gagaaaccgt tccttgccct cgttgctcaa tgccttcgcg cggatgacct tgaccgccgc
     1501 cgggcggtcg agggttgggt cgtgggcgcg atagaccgtg cccatgcctc cttcgccgag
     1561 gacgtcgcga agctcgtagc cgccgaggcg cttgggatcc ataccggcag ggtagcaggg
     1621 gcgggcgagg ggggcaagga tgggggaggc agcgcgccgt cggcgagaca gcgcgccgcc
     1681 ggtattctaa cggctcttct ccatctcctt catcgcttcg tcggtgcgcc ccgtctccgc
     1741 gtagagacgg cccaggatcg cgtgcaacga ggcgttgtcc ggatccgcgg cgacggccgc
     1801 ttgcgtctcc gcgatcgcat ccgcgaccag tccgcggtcg aagaagaggc tcgcgcgcat
     1861 caggtggtac gaggtggcgc cgggcttctt ggcctcgatc gacgcgagat cctggtccag
     1921 cgccgtgacg tcgttgggcg cgatgatctc gaagaacgac gccgcggtgc gcaagggcgg
     1981 cgacaccagc ggatccgacg tctcgagcgt ccacgagtac gagactccgg ggacgagcga
     2041 gggggcgtcg gcggggtacg ccacactcgt cgcaccggag acggtcgact gccagtgaat
     2101 gccggtttcg ttgtagacca cgaactgcag ttcgaggctc gggtcctccg cggtccatgt
     2161 gaacgtcggc cgcgcgtcgc gaaccttggt ctggcagggc gagaccgcgc gcaggtcgct
     2221 cttcttgtcg cccgagcgca ggccgctgat cgagctcgtg ccttcggtgc tcttgagctt
     2281 caagaaattc tgcacgacct cgaagttctg ctctttcggc ttcgcacctg gctgtccctt
     2341 gatcttcatg ctgctgttcg cacccacctg cacccagttt ccggacgcga acatgatctc
     2401 cgcctcggcg tcggcgccgg tcttgacttc gtcgccttcc gagagggccg cgccgaaggt
     2461 cgccgcgacg gggccgttgc ccgcgcgcac gatcgtgacg ggacccttgc atgaggtcag
     2521 caccgcggtc ggcgaatcgt tctgcgcggg agcgggcccg ggagagtggg tgaccacgag
     2581 cacggtcgcg agtccgagta ccaaagcggg gatccagcgt ttcaacatgg cctagctcct
     2641 gatttgtaat atgttgttta ataataactt atgctgcttg ctggggcgga tccacacgta
     2701 ccgccgcact cctactacac ccgagacgag cgggaagttg gcgcgtcacg ggccctcgtc
     2761 catctgatag actggcattc taccgcattc tggagcatcg cgtggtaccg ggccgccgtc
     2821 gaaaccctcg tccataggag tgcatgacat gaaaaaggta ggcatatgga tcgaccatcg
     2881 ccgcgcggtc gtggtgacga tcgagaacgg atcggaatcg atcaagacga tcgaaggcga
     2941 cgtcgatcgg cagccgaagg cggcgggccg gtcgggcaac gccacgaagt gggggccgca
     3001 ggcgccgatc aacgagcgcc gcatcgagga gaactaccgc ctgcatgtcg tgcatttcta
     3061 caaggacgtc atcaagtcga tcgggaagcc ggaccagctg ttggtgatgg gtccggcgca
     3121 ggccaagttg gaattcgcgg aggagatcga gaaagtggcc gaactccgcg gagttccgct
     3181 caaggtcgag accaccgaca agatgaccga cccccaggtc gcggccaagc tgcgcgcgac
     3241 cgagttctag aacctttcgg ggcgcattct cgtatgagga cgacgaggtg attccagtat
     3301 gaaacgtctg tatggaatgg tggcgctgct tctcatgctc gcgtcgtcgg cgttcgcgat
     3361 gagcgtcgaa gagcgcacca agctggagaa cgaagtgaag gcggccgaga acgcgttcgc
     3421 gaagacgctc gcggaccgcg accacaaagc gttcgcgcgg tttatcgcgg atgacgccgc
     3481 cttcttcgga cgtaacagcg tgagtcgcgg caaggaagcg gtcgtcgcat cgtgggcggg
     3541 attattcgaa ggaccgacgc cgccgttttc gtgggcgtcg gaggcggcag tggtgctcga
     3601 gtcggggaca ctggcgctct cttatgggcc ggtgtacgcg gcggacggca agcgctttgg
     3661 caccttcaat tcggtttggc gtcgcgagaa agacggcacc tggaaggtga tcttcgacaa
     3721 gggctgcgat tactgcgaag caggaacgaa atagcgatca cgcggcttgg gcgtcgtcgc
     3781 gcgaccggcg acgcccgcgc tttcccgaca ggtagctcac cagcacgccg gcgccgatca
     3841 tcatgttgag caccagcgtg aagaatcgcc acaagagcac aaacgccgcc tccgacccct
     3901 tcgggatcac cgagcccatc accgcgatcg tcgtcacctc cgcgaagccg gaggcccccg
     3961 gcgacggcga gaagtagaag atcaagagca gcaccacctg catgtagagc acgtgcacca
     4021 ggtcgacggt cccgcccaat ccccgcaata tgacgaacgc gatcacgaac ttgttgaagt
     4081 agatgatcgc cgagagcacc aagccgaacg cgaacagcgt cttgccgcgc accatgaagc
     4141 gcctcatcag ctcgtggtag cggtcgacgg tgtcgaacaa tcgctccacg gcgtgccggc
     4201 tctcgagctt gcgacgtgcc cacggcaggc ttcgcagcag tccgtgcacg gcgcgcttga
     4261 acgcgcccgg gaaaaccagt gagaagagta cgatgaccag gatcaacgcg aacagcccaa
     4321 gggatgcgcc cgaaatgacg cgcaggttgg aacccaagaa gtccggttgc gcgaagatcg
     4381 agaacgagac cgcgcacccc aggagaacga tcacggttcc caggaatccg cccaggaagc
     4441 acgccacggt ggcatcgagc actgtcatcc cttcggccca caagacgtag atctgcgccg
     4501 ccgcgccccc cgattgcgag ggcgtggcgc cacccaggaa cacgttcgcg agacacgaac
     4561 gcatgcacgc accgtaagta atcgcgggat ggattcgatt ggcgaatatg taaatgcgaa
     4621 gcgccgacac cagccaatcg aacactacca agcccacggc cgcggccagc cagcgcggat
     4681 cgagatgggt caggctgcgg attgtatcgc cgaccgacga gcggctgaac acgatgataa
     4741 agccgatcac gctgaacgcg aggaacagct tcaggccggt cttgatcctg ccgacgtgga
     4801 tgggtggcgg tgtcgggggc atggcgttct gcgcagttta cacgatcgtt cgccgacctg
     4861 ctatgatagc ggggccgcgc gccggggcgc gcggtcccgg agggactcgc catgaacacg
     4921 aatcaactca agtctgccct ttcgacgatc gggcaaattg cggtcacggt gcgcgacatc
     4981 ccgcgcgcgg tcaccttcta ccgcgatgtg ctgcgcgtgc cgtttctctt cgaagccccc
     5041 ggcatggcgt tcttccaagc cggtgacgtg cgcctgatgc tgggtctgcc cgagcacgag
     5101 gcgcagcgct tctcgtcgat cctttatttc aaggtggacg acatcaacgg cgtctacggc
     5161 gacctcaagg agcgcggcgt tccgttcacg cacgagccgc gtttgattca tcgcggcagc
     5221 cagaccgatc tgtggctcgc attcttctcc gaccccgacg gcaacaccat ggccctgatg
     5281 agcgaagtcg cccggcgcga ttagggcatt gacgcgcgcg acacccttcc gctagcttcc
     5341 ccgaattcgt agttagcttt tggcaacggg gagtcacgga gggaagcgca tgagagccgt
     5401 cttggtagca gccgtcatcg tggtttgcgg ggtcgcgata cacgccgcgg aggacccagc
     5461 caccactgag aagcgagtcc agcgcggcaa gtatctcgcc gataccatgg gctgtcagga
     5521 ttgtcattct cccaagatct tctcgccgga gggcattccg cacccggacg agtcgaaggc
     5581 gtactccggt caccccggtt cgaagctccc cgcggtggac ccgaaatcgc tcgcgcccgg
     5641 caactggtac ctgatgtcgc ccgacctcac cgcgtacgcc gggccgtggg gaatcagcta
     5701 cgcggtcaac atcacgcccg acgaacagac cgggatcggg ctgtggaccg aggagatctt
     5761 cctccaggcc atccgtaccg ggaagcacat gggcaacggc cggccgattc tcccgcccat
     5821 gccgtggccg tactaccgca acttgagcga cgaggacctg aagtcgctgt ttgcgtacct
     5881 gaagtcgctg ccacccgtta agaacgcggt gccggcaccg gtcgcggcgg ctgacctcca
     5941 gtagccggcc ctttccacgc gtgggcgaat ctggtatagt cgttcgcgtc taacgcgtta
     6001 tcccagcacg acttcgctgg tagcgaacgg cggcgtgtga tccacgccgc cgttgcgcta
     6061 ccatgaccgg gtagccgcgg acgcgcctcg cacggaccca tgaatctcaa cgtctatatc
     6121 gaaacctacg gctgtcagat gaacgtctac gacagcgaac tggtgaagtc gatcctcgtc
     6181 acgcgcggcc accggttggt cgaccggttc gacggcgccg acgtcgtgct gatcaacacc
     6241 tgcgccatcc gcgagaaagc ccaccagaag atctacgggc gcttgcagca gctcaagcgc
     6301 gagcgcgcgc tgcgccgaag cgagctgacc atcgggatcc tcggctgcat ggcccagaac
     6361 ctgaagcagg acctggtcga cggccacgac gaggtggact tcgtcgcggg gccggactcg
     6421 taccggcatt tgcccgagct catcgacgcc gcgcgcggga gtgcggaagc gactcccgcg
     6481 ttggcgggcg cggccctcaa gttgtccacc accgaaacct acaccggcgt cgacccggtg
     6541 cgcagcggcg gcatcaacgc gtggatcgcg gtcatgcgcg ggtgcgacaa catgtgcacg
     6601 ttttgcgtgg tgccctacac gcgcggccgc gagcgctctc gcgaccccga aggcgtcatc
     6661 gacgaagtgc gcgccctggt gcgcgacggc tacaaacagg tgacgctgct cgggcagaac
     6721 gtcaactcgt accgcttcgg cgaggttcgc ttcgcggact tgatgcggcg ggtggccgac
     6781 gtgcccggcg tcgagcgcgt gcgcttcatt tcgccccacc cgaaggactt cccggtcgaa
     6841 ttgattcgcg ccatcgccga gcaccccaag ctgtgcaagc acatccacct gccgctacag
     6901 gccgggagcg accgcgtgct cgagctcatg cggcgcacct acaccaatgc cgagttccgc
     6961 gccctggtcg acgagatgcg gggcacgatc gcgaatctct cgctgaccac cgacgtcatc
     7021 gtcggcttcc cgaccgagac cgcggccgag ttcgacgaga ccatgcgggt gatcgacgac
     7081 gtgcgtttcg acgccgcctt cacgttcaag tactcggagc gcaagggcac ctacgcgcag
     7141 cgaaagctgc gcgacgacgt cgccgccgag gagaaaacga agcgaatcgt cgcgctcgtt
     7201 gatctccaga agcgaatcac cgatgaagtg aatagggggc ttatcggctc gacccacgac
     7261 gtgctggtcg aagagcacga cgagaagcgc cccggcttcg tggtcgggaa gaccgaccat
     7321 ttcaagcaca cgattctgcc ggccgacggc gccgagatcg gcgacacggt ccgcgtcgta
     7381 gtcgagaacg cccacggtgc cacactgtac ggacgtgcgc tctcgacgca cgcgagaccg
     7441 gcgcggcgcg cggtcg
//
LOCUS       DASPGK010000445         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1633047, whole genome shotgun sequence.
ACCESSION   DASPGK010000445 DASPGK010000000
VERSION     DASPGK010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633047"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VEC56_12525"
     CDS             <1..813
                     /locus_tag="VEC56_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXV15023.1"
                     /translation="VAAGSLVVREPVAAAQSEVDALRPRVDVVVVLAHMGRSEIKRVA
                     ESLTGVDVVVNGHDGIPMRQVRRWGEPYVVQIAAKGMYVGVADATLGKDKRVARLKNA
                     VVGLDKTFRDDEAVAKLFQAYDMEVVAREKASLPVGAAVTFTGADACRSCHVPIFEQW
                     KGTRHAHAFEVLTAQKREFDRDCTPCHTTGFFKQGGFVNASATPHLSGVQCEACHGNG
                     TAHAKDPKVKTDTVAKSTCRSCHTAEQTPEFDFATFWARIDHGSGSTAAGSR"
     gene            865..2028
                     /gene="alr"
                     /locus_tag="VEC56_12530"
     CDS             865..2028
                     /gene="alr"
                     /locus_tag="VEC56_12530"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXV15024.1"
                     /translation="MKRLPTWLEIDLDRLDRNLEQIQRWVGDDVRMLLTVKADAYGHG
                     AVQVAHAAQARVHVFGVATVDEAVELSDSGIKNQILILSPILETEIAAVARHGFAITL
                     SGADFADAIAHHAVPPIPVHVEVDTGMGRTGVPEEDAFDEIRRVAATRAVRLEGVYTH
                     FPVSDSDPEFTRAQVERFAALIAKLRASGVIIPIVHSANSAAVDGVRDAHLNLVRPGL
                     LAYGLHPSGAQPGVDVAPIMSWKSRIVRVRRVAKGKSISYARSFVTERASMIGVVPVG
                     YGHGYPFHLSGRGSMLVGGHRVPIVGRVTMDMTMVDLTDLAETPRAGDEVVLVGTQGN
                     QSLTFHDLAAWGDTICYEIMCGISKRVPRTYFRRGKVETFKSLLGVIPNHATA"
BASE COUNT          349 a          702 c          732 g          335 t
ORIGIN      
        1 gtcgccgcgg gctcgctcgt ggttcgcgag ccggtggccg ccgcgcagtc ggaggtggac
       61 gcgcttcgcc cccgcgtgga cgtggtggta gtcctggcgc acatgggccg cagcgagatc
      121 aagcgcgtcg cggaatcgct cacgggcgtc gacgtggtcg tcaacggcca cgatggtatc
      181 ccgatgcggc aagtcaggcg gtggggcgaa ccctacgtgg tgcagatcgc ggccaagggg
      241 atgtacgtcg gcgtggccga cgcgaccctc ggcaaggaca agcgcgtggc ccggctcaag
      301 aacgcggtgg tcgggttgga caagacgttt cgcgacgacg aggccgttgc gaagctcttc
      361 caggcgtacg acatggaggt cgtcgcgcgc gagaaggcgt cgttgccggt cggggccgcg
      421 gtgaccttca cgggcgccga tgcgtgccgg tcgtgccacg tccccatctt cgagcagtgg
      481 aaaggcacgc gccacgcgca cgcgttcgag gtgctcacgg cgcagaagcg cgaattcgat
      541 cgcgactgca caccctgcca cacgaccggc ttcttcaagc agggcggctt cgtcaacgcg
      601 agcgccaccc cgcacctctc cggcgtccag tgcgaagcgt gccacggcaa cggcaccgcg
      661 cacgcgaagg accccaaggt caagaccgac acggtcgcca agtcgacgtg ccgcagctgt
      721 cacaccgccg agcagacccc ggagttcgat tttgcgacct tctgggcccg aatcgatcac
      781 gggagcgggt cgacggcggc ggggtcacgc tagcggcgag caatcgcttg acccgatttc
      841 accccgcaag gtagcgtcga cgccatgaag cgccttccca cctggctcga gatcgatctc
      901 gaccgcctcg accgcaacct cgaacagatc cagcgatggg tgggtgacga cgtccgcatg
      961 ctgctgacgg tcaaggcgga tgcgtacggc cacggcgcgg tccaggtcgc gcacgccgcc
     1021 caggcgcgcg tgcacgtgtt cggggtcgct accgtcgacg aagccgtcga gctgtccgac
     1081 tccggtatca agaatcagat tctcatcttg agcccgatcc tcgagaccga gatcgcggcc
     1141 gtggctcgcc acggcttcgc catcacgctc tcgggggccg atttcgccga cgccatcgcg
     1201 caccacgcgg tgccgccgat ccccgtgcac gtcgaggtcg acaccggcat gggccggacc
     1261 ggcgtgcccg aggaagacgc cttcgacgag attcgtcgcg tcgcggccac gcgcgccgtg
     1321 cggctggagg gcgtgtacac ccattttccc gtctccgaca gcgaccccga gttcacgcgc
     1381 gcgcaggtgg agcgcttcgc cgccttgatc gcgaagctgc gcgcctccgg cgtcatcatt
     1441 ccgatcgtgc acagcgccaa cagcgccgcg gtggacggcg tgcgcgacgc gcacttgaat
     1501 ctggtgcgcc cgggactgct cgcgtacgga ttgcacccgt cgggggcgca gcccggcgtc
     1561 gacgtcgcac ccatcatgag ttggaagtcg cgcatcgtgc gcgtgcgccg agtggccaag
     1621 gggaagtcga tcagctacgc gcgctcgttc gtcaccgagc gcgcgtcgat gatcggcgtg
     1681 gtgccggtcg ggtacggcca cggctatccg ttccatttat cggggcgggg ctcgatgctg
     1741 gtgggcgggc atcgcgttcc catcgtgggg cgcgtcacca tggacatgac catggtcgat
     1801 ctgaccgacc tggcggagac gccgcgcgcg ggcgatgaag tggtgctggt ggggacgcag
     1861 gggaaccagt cactcacctt ccacgacctc gcggcctggg gcgacaccat ctgttacgag
     1921 atcatgtgcg gaatctcgaa gcgggtcccg cgcacgtatt tccgtcgcgg taaggtggag
     1981 acattcaagt cgctgttggg agtgatcccg aaccacgcga cggcgtgacg gcaaagagtc
     2041 cggccgaagc gatcgatcct tcgaacgcga tttctgcagc gcgaacagga cacgatactg
     2101 tccggctcgc gctgcagt
//
LOCUS       DASPGK010000446         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_813378, whole genome shotgun sequence.
ACCESSION   DASPGK010000446 DASPGK010000000
VERSION     DASPGK010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813378"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VEC56_12535"
     CDS             complement(<1..650)
                     /locus_tag="VEC56_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV15025.1"
                     /translation="MRRSSEKKHWEEFWSSSPGLDDVYANDGRVVAFLASRLDLAGQR
                     VLEVGAGTGRDSVALSALGAQVWTLDYSDESLRIMREASAGERSAGEELRIVAGDALA
                     LPFRDQSFDVVFHQGLLEHFRDPLALLRENHRVLRPGGYLLVDVPQRYHYYTLMKHAL
                     MALDRWFAGWETEFSARELASLVRAAGFDVVGDYGENLFPPVWYRGARRVLLKAGLR"
     gene            complement(670..1407)
                     /locus_tag="VEC56_12540"
     CDS             complement(670..1407)
                     /locus_tag="VEC56_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXV15026.1"
                     /translation="MKYAIFSDVHGNLESLEAVLAHAAGQGVGFHLCLGDVIGYGANP
                     NECVDRVRALPAVACLKGNHDAAVVDARERAYFHEVALEGIHYSALRLTPDNAEFLRT
                     LPYVYRDHARVMAVHASPYRPEDWEYVLDQVGAERAFQAMAPYRAAFIGHSHSPVVFV
                     DDGTAQRFPNDGPLMLDLAKHRYVINVGSVGQPRDGNPDASYVVFDDQTDAARLFRVR
                     YDREKAAEKILKAGLPPVLAERLLVGF"
     gene            complement(1404..1820)
                     /gene="nusB"
                     /locus_tag="VEC56_12545"
     CDS             complement(1404..1820)
                     /gene="nusB"
                     /locus_tag="VEC56_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXV15027.1"
                     /translation="MGKRRRAREIVLQALYEAEFSERSTHDIIEEQIGRRTPSDETAQ
                     YARALFLKTIEKKSDLDGVIQSLLENWDPSRVSLVDRNILRFALAEVLYFPDVPGRVI
                     IDEAIEVAHRFSSEEAGRFVNGLVDRFVKEFRRDGA"
     gene            complement(1824..2285)
                     /gene="ribH"
                     /locus_tag="VEC56_12550"
     CDS             complement(1824..2285)
                     /gene="ribH"
                     /locus_tag="VEC56_12550"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXV15028.1"
                     /translation="MPNEVRGRFDATGRRVAVVASRFNEVVTRRLVEGAVAALSQHGV
                     SPEAIDIYWVAGAFEVPQLAAKVARDGKADGVVWAGAIVRGETDHYEVLSRAVTSAIE
                     STAVSTGVPMTLAVLTTDTVEQAIDRAGGKHGNAGWNAAVALVELMSQFRN"
     gene            complement(2275..3522)
                     /locus_tag="VEC56_12555"
     CDS             complement(2275..3522)
                     /locus_tag="VEC56_12555"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HXV15029.1"
                     /translation="MSRFSNIERAIEVIRSGGIVIVVDDADRENEGDMILAAERATAE
                     KINFLARYARGLICAPLMPERAEALRLLPMVPDNTCKLGTAFTVSVDLVGGTTTGISA
                     HDRAATIRALVDPSTRPEDFARPGHVFPLVAKPGGVLRRAGHTEAAVDLARMAGLAPV
                     GVLCEVLDDDGSMARLPRLQEIATELDLEMVTIADLIAYRRRNERLVERIESIRFPTE
                     YGEFTLHLYRSLASGHNHIALVKGTIQRDQPALVRVHSQCFTGDVFGSLRCDCGQQLH
                     QAMAKIEEAGSGVLLYMNQEGRGIGLENKIRAYALQDAGHDTVEANEALGFPADLRDY
                     GVGAQILGDLGVGKILLLTNNPKKVVGLSAHGLEVVDRVPIEILANEVNRGYLAAKRD
                     KLGHLLSQTLAAAGPRTRGKHAQ"
BASE COUNT          568 a         1224 c         1190 g          576 t
ORIGIN      
        1 cgcaggcccg ccttcaggag cacgcggcgc gcgccgcgat accacacggg cggaaacagg
       61 ttttcgccgt agtcgcccac gacgtcaaag ccggccgcgc gcacgagcga cgccagctcg
      121 cgcgcggaga attccgtttc ccagccggca aaccagcgat cgagcgccat cagcgcgtgc
      181 ttcatgagcg tgtagtagtg gtagcgctgg ggaacgtcga ccaagaggta tccgccgggg
      241 cgcaacacgc ggtggttctc gcgcaagagc gccaacggat ctcggaagtg ctcgagcaat
      301 ccctggtgga acacgacgtc gaaggactga tcgcgaaacg ggagggccag ggcgtcgccc
      361 gctacgatgc gcagctcttc gcctgcggac cgctcgcccg cggacgcctc gcgcatgatg
      421 cgcagcgact cgtcgctata gtcgagggtc cacacctggg cgcccagcgc ggacagcgcc
      481 acactgtcgc gcccggtgcc cgcgcccact tccagcacgc gctggcccgc gaggtcgagc
      541 ctggaggcga gaaacgcgac cacgcggccg tcgttcgcgt acacgtcgtc gagccccggc
      601 gacgacgacc agaattcttc ccagtgcttc ttttcggacg aacgccgcat gcgggaatcg
      661 accacgcgct cagaagccga ccagcagtcg ctccgccagg acgggcggga ggccggcctt
      721 gaggatcttc tcggccgcct tctcgcggtc gtagcgcacg cgaaacaggc gcgcggcgtc
      781 ggtctggtcg tcgaatacca cgtaggacgc gtccggattg ccgtcgcggg gctggcccac
      841 gctgcccacg ttgatcacgt aacggtgctt ggcgaggtcg agcatgagcg gcccgtcgtt
      901 ggggaaccgc tgcgcggtgc cgtcgtcgac gaatacgacc ggcgagtgcg agtggccgat
      961 gaaagcggcg cggtacgggg ccatggcctg gaaggcgcgc tcggcgccca cttgatccag
     1021 gacgtactcc caatcttcgg gtcgataggg agacgcgtgc accgccatga cgcgcgcgtg
     1081 gtcgcggtac acgtagggca gcgtgcgcag gaactccgcg ttgtccggtg tcaggcgcag
     1141 cgcgctgtag tggatgcctt cgagcgccac ctcgtggaag tacgcgcgct cgcgcgcgtc
     1201 caccacggcc gcgtcgtggt tgcccttgag gcacgcgacc gcgggcagcg cgcgcacgcg
     1261 gtcgacgcac tcgttggggt tggcgccgta tccgatgacg tcgcccaggc acaggtggaa
     1321 gccgacgccc tgcccggccg cgtgcgcgag aaccgcttcc agcgattcca ggttcccgtg
     1381 cacgtcggaa aagatcgcgt acttcacgcg ccgtcccttc tgaattcctt gacgaaccta
     1441 tcgaccaaac cgttcacaaa gcgcccggct tcttccgagc tgaagcgatg cgccacttcg
     1501 atggcttcgt cgatgatcac gcgcccgggc acgtcgggaa aatagagaac ttccgcgagc
     1561 gcgaagcgca ggatgttgcg atcgaccagc gacacgcggc tcgggtccca gttctcgagc
     1621 agcgactgga tcacgccgtc gaggtcggat ttcttctcga tggtcttcaa gaacagtgcg
     1681 cgcgcatact gcgcggtttc gtccgacggg gtgcggcggc cgatctgctc ctcgatgatg
     1741 tcgtgcgtcg agcgctccga gaactccgcc tcgtacaacg cctgcaacac gatctccctg
     1801 gccctccgtc gtttccccat cggtcagttc cggaactggc tcatcagctc gaccaacgcg
     1861 accgccgcgt tccaccccgc gttgccgtgc ttgccgccgg cacggtcgat cgcctgctcg
     1921 acggtgtcgg tcgtgagcac cgcgagcgtc atgggaacac cggtcgacac cgccgtcgat
     1981 tcgattgcag aagtcacggc gcgcgacagc acttcgtagt ggtcggtctc gccgcgcacg
     2041 atggcacccg cccacaccac gccgtcggcc ttgccgtcgc gcgcgacctt ggccgcgagc
     2101 tgcgggacct cgaaggcacc ggccacccag tatatatcga tcgcctcggg agatacaccg
     2161 tgttgagaaa gtgccgccac cgccccctcg accaggcgcc gggtcacgac ctcgttgaag
     2221 cggctcgcca ccaccgccac gcgccggccg gtggcgtcga agcggccgcg cacctcattg
     2281 ggcatgcttg cccctcgtgc gcgggcccgc agccgcgagc gtctgggaga gcaggtggcc
     2341 cagcttgtcg cgcttggcgg cgaggtagcc gcggttgacc tcgttggcca aaatctcgat
     2401 gggaacgcgg tcgacgacct cgagcccgtg cgccgacagg cccaccacct tcttggggtt
     2461 gttggtcaac agcaggatct tccccacgcc gaggtcgccg aggatctgcg cgcccacgcc
     2521 gtagtcgcgc aggtcggccg ggaatcccag ggcctcgttc gcctcgacgg tgtcgtggcc
     2581 ggcgtcctgc aacgcatacg cgcgaatctt gttctcgagg ccgatgccgc ggccctcctg
     2641 gttcatatag aggagcacgc cggagccggc ctcttcgatc ttggccatgg cctggtgcag
     2701 ctgctggccg caatcgcagc gcagcgaccc gaagacgtcg ccggtaaagc actgcgagtg
     2761 cacgcgcacc agcgcgggtt ggtcgcgctg gatggttccc ttgaccagcg cgatgtggtt
     2821 gtgaccgctg gcgaggcttc gatacaagtg gagcgtgaac tcgccgtact cggtgggaaa
     2881 gcgaatcgac tcgattctct ccacgaggcg ttcgttgcga cggcggtacg cgatcaggtc
     2941 ggcgatggtg accatctcca ggtcgagctc ggtcgcgatc tcctgcaggc gcggcaggcg
     3001 cgccatggag ccgtcgtcgt cgagcacctc gcacaggact cccaccggtg ccaggcccgc
     3061 catgcgagcg aggtcgaccg cggcctcggt gtgacccgcg cggcgcagta ctccgcccgg
     3121 cttggcgacc agcggaaaca cgtgacccgg ccgcgcgaag tcctcggggc gtgtcgacgg
     3181 atcgaccagc gcgcggatgg tggccgcgcg gtcgtgcgcg ctgatgccgg tcgtggtccc
     3241 gcccaccaag tcgaccgaga ccgtgaacgc ggtccccagc ttgcacgtgt tgtcggggac
     3301 catcggcagg agccgcaggg cctcggcgcg ctcgggcatg agcggggcgc agatcaaccc
     3361 gcgcgcgtag cgcgccagga agttgatctt ctccgcggtc gcgcgctccg cggccaggat
     3421 catgtcgccc tcgttctcgc ggtccgcgtc gtcgacgacg atcacgatgc cgccggaacg
     3481 aatgacctcg atcgctctct cgatgttgga aaacctgctc atcgcttctc ctgtctctgg
     3541 ggcgcgcagc ccctgggt
//
LOCUS       DASPGK010000447         3609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_930729, whole genome shotgun sequence.
ACCESSION   DASPGK010000447 DASPGK010000000
VERSION     DASPGK010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930729"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..908
                     /locus_tag="VEC56_12560"
     CDS             120..908
                     /locus_tag="VEC56_12560"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HXV15030.1"
                     /translation="MNTETARPEMVVRVENMDDGAIRRVVLNTPRANILDRDKTVALT
                     HVFEKAREDARVKAIVLEGEGPNFSFGASVQEHLPESCAAMLAAFHGLFRAMLDASVV
                     TLAAVRGNCLGGGLELAAFCNRVFAAGDARLGQPEITLGVFAPVASVILCERMGRGGA
                     EDLLLSGRTVTAAEAMRLGLVDEVDDDPFEAALSYARAHLLPRSASSLRWAVRAARLG
                     FGDRLVAELGRLEAMYLEDLMPTADANEGLRAFLEKRTPAWRDQ"
     gene            905..2086
                     /gene="bcrC"
                     /locus_tag="VEC56_12565"
     CDS             905..2086
                     /gene="bcrC"
                     /locus_tag="VEC56_12565"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HXV15031.1"
                     /translation="MSAGASSRLSAVLQRAEDLYRDRTLGEVRAWKARTRGLAIGFMP
                     IYIPREMLHAQGVMPVGIMGGGDDLEIIRGDAYYQSYICHIPRSTIEMGLNGSLDCLD
                     GMIFPATCDVIRNLSGMWQLQFPDRLSCYLDVPQNFDAALGGSFYRRELEHLSHELVA
                     HGARPLEPEVLRASIVTYNENRRRVETLFGLRRRAPWKIPTHELYLLLRAGQVMAVED
                     FTAMLEEYITAVNEDTTRQPLDQARVLLTGSFCEQPPLGLIKTLERSGCYIVEDDFVQ
                     VHRFIRGEVRVEGDPMDNIVRAFVEDGVASPVRYIAEHEKGAELVARVRECAAEGVIY
                     CSASFCDPALLDQPMTVRALEKAGIPYTAFKFAENNGQFQVIREQSGTFADSIKLWSE
                     V"
     gene            2089..3378
                     /gene="bcrB"
                     /locus_tag="VEC56_12570"
     CDS             2089..3378
                     /gene="bcrB"
                     /locus_tag="VEC56_12570"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HXV15032.1"
                     /translation="MAPKTETQVHKEDSQLRQKTMIAEHYTRLANTPDTGEKSVYTFV
                     PGNLTELIRSFDLLPVLPEINALQSAMRGKSREYIAIAEKLGHSEDVCTYVKCDLGMM
                     KSGNVGPTGERLPKPDLLLLSYTGCFTFMKWFEILREEYDCPVAMLHVPYQAGGVITD
                     SMRTYVVEQLRDKVIPLLEQVSGKRYDEARLRAMLTRSARAEDDLVAVLNAARSRPSP
                     IDAYFGGVYYIGPIFSAFRGTEDAVDYYRMLRDEVEERAREGLGPVTPEGPMERERFR
                     LVVEGPPNWTNFREFWKMFADEGAVVVASTYTKVGGVYDMGFRHDPNRPLESLAEYCL
                     GCYTNLSLPRRVEMLARYVQDFDADGLLVNSIKSCNSFSAGQLMILREVERRTGRPGG
                     FIESDLVDPRYFSAANIKNRLESYLQMIEQKRAGRTA"
     gene            3375..>3609
                     /locus_tag="VEC56_12575"
     CDS             3375..>3609
                     /locus_tag="VEC56_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15033.1"
                     /translation="MKICVGIDLGSTTTKAVILGDAGETLGRGITNSRSNYKVACDVA
                     LGEAWRVRPEGPLIERLGAWLAPENVQVVYGVYG"
BASE COUNT          616 a         1164 c         1242 g          587 t
ORIGIN      
        1 ttcttgctgc tgcgccggcg ccttgcggag gggcgcgtat gggacgacgc gctcatcgac
       61 gagatccagc cccaacccga cggcgccggc agccgcaagg tctgaggagg ggagcgggca
      121 tgaacaccga aaccgcgcgg ccggaaatgg tggtgcgcgt tgagaacatg gacgacggtg
      181 ccatccggcg cgtggtactc aacacgcctc gcgcgaacat tctcgaccgc gacaagaccg
      241 tggcgttgac gcacgtcttc gagaaggcac gcgaagatgc gcgcgtgaag gcgatcgtct
      301 tggagggtga ggggccgaat ttctccttcg gcgcgagcgt gcaggagcat ctccccgagt
      361 cgtgcgccgc gatgctggcc gcgttccacg gactgtttcg cgccatgctc gacgcatcgg
      421 tggtgacgct ggccgccgtg cgcgggaact gcctgggcgg cgggctcgag ctggcggcgt
      481 tctgcaaccg cgtctttgcg gccggggacg cgcgcctcgg ccagccggag atcacgctcg
      541 gcgtctttgc gccggtcgcc tccgtgatcc tgtgcgaacg catggggcgc ggtggtgcgg
      601 aggacctgct cctctcggga cggaccgtca ctgccgcaga ggcgatgcgg ctcggcctgg
      661 tcgacgaggt cgacgacgat ccgttcgagg ccgccctgtc gtacgcgcgc gcgcacctct
      721 tgccgcgttc ggcatcgagc ctgcgctggg cggtacgcgc ggcgcggctc ggattcggcg
      781 atcggctggt cgcggagctg ggacggctgg aggcgatgta tctcgaagat ctcatgccca
      841 ccgccgatgc gaacgaagga ttgcgggcgt ttctcgagaa gcgaacgccg gcgtggaggg
      901 accaatgagc gcgggcgcct cgtcgcggct gtccgcggtc ctgcagcgcg cggaagatct
      961 gtaccgcgat cgcacgctgg gtgaggtgcg cgcgtggaag gcacggaccc gaggcctggc
     1021 catcggcttc atgcccatct acatcccgcg cgagatgctg cacgcgcagg gcgtcatgcc
     1081 ggtcggcatc atgggaggcg gggacgatct cgagatcatt cgcggcgacg cgtactacca
     1141 gtcgtacatc tgccacatcc cgcgcagcac catcgagatg ggactcaatg gatcgctgga
     1201 ctgtctcgac ggcatgatct tcccggccac ttgcgacgtg atccggaacc tctcgggcat
     1261 gtggcagctc cagtttccgg accggctctc gtgttacctg gacgtgccgc agaacttcga
     1321 cgccgcactc ggtggtagtt tctaccgccg ggaattggag cacctctcgc acgaactcgt
     1381 cgcgcacggg gcgcgcccat tggaaccgga ggtcctgcgc gcctccatcg tgacctacaa
     1441 cgagaaccgc cgccgcgtcg agacgctgtt cggtctccga cgacgcgcgc cctggaagat
     1501 cccgacgcac gaactctacc tcctgctgcg tgccgggcag gtgatggcgg tggaggactt
     1561 caccgcgatg ctggaggagt acatcacggc ggtgaacgag gacaccacgc gccagccatt
     1621 ggaccaggcg cgggtgcttc tcaccggctc gttctgcgaa cagccgccgc tgggtctcat
     1681 caagacgctc gaacgctcgg gttgctacat cgtggaagac gatttcgttc aggtgcaccg
     1741 attcatccgc ggggaggtgc gcgtggaagg cgatcccatg gacaacatcg tgcgcgcgtt
     1801 cgtcgaggac ggcgtagcga gcccggtgcg ctacatcgcc gagcacgaga agggagccga
     1861 gctggtggcg cgcgtgcggg agtgcgcggc ggagggcgtg atctactgct cggcgagttt
     1921 ctgcgacccc gccctgctcg accaaccgat gacggtgcgc gcgctggaga aggcgggtat
     1981 tccctacacc gccttcaagt tcgccgagaa caacgggcag ttccaggtga tccgcgaaca
     2041 atcggggacc ttcgcggact cgatcaaact gtggagcgag gtgtaaccat ggcgcccaag
     2101 accgagacgc aggtgcacaa ggaagacagc cagcttcgcc agaagaccat gatcgcggag
     2161 cactacacgc gcctcgccaa cacccccgac accggcgaga agagcgtgta taccttcgta
     2221 cccggcaacc tcacggagct catccggagt ttcgaccttc tgcccgtgct gccggagatc
     2281 aacgcgctgc agtcggcgat gcgcggaaag tcgcgcgagt acatcgcgat cgccgagaag
     2341 ctcggtcatt ccgaggacgt gtgcacgtac gtgaagtgcg acctgggaat gatgaagtcc
     2401 ggcaacgtcg gccccaccgg cgaacggctt ccgaagcccg acctgctgct gctctcctac
     2461 accggctgct tcacgttcat gaagtggttc gagatcctgc gcgaagagta cgactgcccc
     2521 gtggccatgc tgcacgtccc ctaccaagcc ggcggtgtga tcacggattc catgcgcacg
     2581 tacgtggtgg agcagcttcg cgacaaggtc atcccactcc tcgagcaggt gtccgggaag
     2641 cgctacgacg aggcgcgcct gcgcgccatg ctgacgcgct cggcgcgcgc cgaggacgac
     2701 ctggttgccg tgctcaacgc ggcgcgcagc cgtccctcgc cgatcgatgc gtacttcggg
     2761 ggcgtgtact acatcggtcc catcttcagc gcgttccggg gcaccgagga cgcggtcgac
     2821 tactaccgca tgctgcgcga cgaggtggag gagcgggcgc gcgaggggct cggcccggtc
     2881 acacccgagg gaccgatgga gcgcgagcgc ttccggctgg tggtggaagg gccgcccaac
     2941 tggaccaact tccgcgagtt ctggaagatg ttcgcggacg agggcgcggt agtggtggcg
     3001 tcgacctata ccaaggtggg gggcgtgtac gacatgggct tccgccacga ccccaaccgc
     3061 cccctcgaga gcctggcgga gtactgcctg ggctgctaca ccaatctgtc gctcccgcgg
     3121 cgcgtggaga tgctcgcgcg ctacgtgcag gacttcgacg ccgacggact gctcgtcaac
     3181 tccatcaaga gctgcaactc gttcagcgcg gggcagctca tgatcctgcg cgaggtcgag
     3241 cgtcgcaccg ggcgtccggg cggcttcatc gagagcgatc tcgtcgatcc gcgctacttc
     3301 tccgcggcca acatcaagaa ccggctcgag tcgtacctgc agatgatcga gcagaagcgc
     3361 gccgggagga ccgcatgaag atctgcgtcg gcatcgacct gggctcgacc accaccaagg
     3421 ccgttattct gggcgacgcg ggcgagaccc tgggccgcgg catcaccaac agtcgcagca
     3481 actacaaggt ggcctgcgac gtcgccctgg gcgaggcctg gcgggtgcgg ccggaaggcc
     3541 cgctcatcga gcgcctcggc gcgtggcttg cgccggagaa cgtgcaggtg gtctacggcg
     3601 tctacggca
//
LOCUS       DASPGK010000448         8672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_579399, whole genome shotgun sequence.
ACCESSION   DASPGK010000448 DASPGK010000000
VERSION     DASPGK010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579399"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..592
                     /locus_tag="VEC56_12580"
     CDS             17..592
                     /locus_tag="VEC56_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXV15034.1"
                     /translation="MKPRIIAFVVASLFVGSQAGASILSASSQAVREPSIEAAGQWTA
                     TTLQNDLNASLRDASDASSAAGPLASTRTPVTGGLVVNGGGTGASYTPFSGSAAFDGL
                     FEMNQESGTVTRGSWDPSGPVIASSGGRGGRDRDRDRDRGRGRYRDRDRPRDRPNPYA
                     TPEPSTWVLLGSGLLAIGAFAGLRRRQALSF"
     gene            754..1914
                     /locus_tag="VEC56_12585"
     CDS             754..1914
                     /locus_tag="VEC56_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HXV15035.1"
                     /translation="MSTGTLYFVLREGSTREEQWSWEEIENLCRSGDLTRRARIFLPE
                     ENRWAELGETRLAAAAAGSKPQEAPEDDAASEAKKKLEAEYQAALEQAEAAPDDLEAR
                     LDAGVIAAQLGNRDEAKNHFQSVLHRYPYHARAAQEVKRRFSKTELRTFRYLDRPAPI
                     WEDLGQLAAMPLARGPLYVIVPAAVIAGLSFVRFGGILASALVFLWMFQVMEYTARGA
                     NKPPEWDRSFADPWRKLGRPSLLMAVVALQLGVIVFAAAKGVMLIEGVKDQTLWRYIA
                     GSPIFVVAASIVLALYLPAAFVSIGGFAGPVAKTLDPRRLVKTIIRMEHEYIYSVVIV
                     AAIGIVAAIVCGITAGAPVAGDMVRGAVLAYATPAVGLVLGRLLGRVGHVIA"
     gene            complement(1959..2507)
                     /gene="pyrE"
                     /locus_tag="VEC56_12590"
     CDS             complement(1959..2507)
                     /gene="pyrE"
                     /locus_tag="VEC56_12590"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXV15036.1"
                     /translation="MTLRDELLQHLLAHSFRTGEFTLASGRKSNYYINGKMTTLDARG
                     AYLVGRTFLAMVADDVPDAVGGLTLGADPIVGAMIALAGLEDLPLKGFIVRKEAKGHG
                     TQSLVEGSLSKGERVVIVEDVVTTGGSSLQAIAAVKALGCEVRKVLAVVDREEGGREA
                     LKQEGLVLEAVFTARELMAAVK"
     gene            2647..4698
                     /locus_tag="VEC56_12595"
     CDS             2647..4698
                     /locus_tag="VEC56_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV15037.1"
                     /translation="MASILDGIERALVAIDRTLAQGRRVPLLLFIAALALKIGYVAHT
                     ADALYVKVPIMDARYYDTMAQDIARGHVVRDEAFFMGPLYPYFLALVYSTVGRDFMWV
                     RVLQAAGGALTVTLAFLIGRRVFRPSAALVGAALLALHGAMTFYETELLMEWMGALLN
                     AAALLLLLRAPAATPRTYAAAGAILGLSALARASILVFAAVVLGWIVRVESGRRRVRS
                     VLAFGLAIVAVLAPAVVHNLAVSRDFVPVTSNAGINFYIGNSLAANGTFVPIRGVDEI
                     DDVTTSEYVERATGRAATPSEVSHYWFERAMTDVRADPARSLRLMARKVILFFNGYEV
                     PQIESFDVHAAESTWLRALFVSTWFVVAVGILGMVLAARSRRGSGLLSGYVLAYAASI
                     VVFFVTGRYRSQVVPVLCLFAGFALVSLPALARRPRAAAAAAFALLLLFLATNPSIFR
                     LDESMVMYREYVHRGRRLSELRSYAPALREIDRAIAIYPKQAEGYVQRAVVHKENGDD
                     FKAIEDYSRALDIEPAAPSVHYDLAQALRRVNLPGEAIREYQLAVRYDPNMVQAYNNM
                     GVTLREMGRFEEAVAAFRKVTEIAPNYAKAYNNLGACYAEMGRPEDAIAVFEETTRRF
                     PEYPNGFKNLAMAYASQKRVRPALAAMRRYALLEPADAEAKEIVRKLEIAAAADSASS
                     N"
     gene            complement(4695..5342)
                     /locus_tag="VEC56_12600"
     CDS             complement(4695..5342)
                     /locus_tag="VEC56_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15038.1"
                     /translation="MRARVLALLVLVVLVGAHARPARALDDLAAQERLGARIGYVETY
                     DGVYQYFGPGWDVLLYFNERIHSRLFLDLHVGAIYLGDILDPELDDFITRYTNVESEM
                     RMFYFSLGLAYGFPLGKSPYTLTTSLAAGVYSVSVAFVSDFVSDDVSDSYFGGNGSIG
                     LMRRLGTGWALEANCTLHYFDTSAGYSDLLWVFTATEAVDPVLFGVSLGLAVDLR"
     gene            complement(5413..7179)
                     /locus_tag="VEC56_12605"
     CDS             complement(5413..7179)
                     /locus_tag="VEC56_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV15039.1"
                     /translation="MVTITTAVAVAWALAPVPAGAEFVRYDFEGTLFSEAPQPVLDHC
                     VVEDNGVYHLFYLRGNPAVNIGHAITTDFVHWDIEPPVLTLGTWDNKAMWAPFLLPLA
                     TGGWAMFYTGVNLLNSQQAGISWSTTMYDWYKWPDPIYHPDPVWAEWTETGFAHGRDP
                     HVVEYQGRYYMFNTAKTNTNRGAVSCAVSDNLLDWEDIGPIYVHDSWHVLESVFILQR
                     PNGKWHMFFTEETVAGTSHMYSDSLLSGWDIGTRRVIDFGHAPQITDTHIGQLFSRHA
                     VYNDAHGTQRYVFRFTPMVWLNDQAVVPRPLVLSGAWTFVPGSGDGFYYQPTFGHNAF
                     VRNENYAATFQGDFWINSYEYYTGPLGYGTPGQYFGDPKMGIIRSNPFTIEGNSMNLL
                     VGGGDYPSQCYVALVDAQTTEVLFKESGRNSNEMDRRYWSLTPHIGRSVYIEIADLST
                     SPFGHICVDDIVESGDVLLPGTGGSGRGKQVPSSRTQASADPRPGATTRLLPNTPNPF
                     NPATTIGYELARDSDVRVDIFDARGAHIRTLVDGRESAGAHRVAWNGTDGRENPLASG
                     VYFYRLTVDGSAVETRKMALLK"
     gene            7630..8361
                     /locus_tag="VEC56_12610"
     CDS             7630..8361
                     /locus_tag="VEC56_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXV15040.1"
                     /translation="MAIAGPVVSFAIAAILFAGLALAGTSPHGDGLLVDLAWANLALG
                     LFNLTPAFPMDGGRILRGALATRMSYLDATRRARAVGQLLALGFATLAFLDFTFAMLS
                     VIAVLVFVGGMLEERAIRARVVLSGRCVGDLVDAETPVLSADDRVVSATRRVGTLAAP
                     AYAVAGEAGSLAGVVAASDLMRALRDGRADQDLLSIARRDFPVAEASTDASRVYRYLR
                     ESNKPFAAVVDGDRFVGLFHAADGW"
BASE COUNT         1366 a         2898 c         2920 g         1488 t
ORIGIN      
        1 caaaaggatt cgaatcatga agccccgcat cattgcattc gtcgtcgcga gcctcttcgt
       61 gggttcgcag gccggcgcat caatcctctc cgcgtccagc caggccgtcc gcgagccgtc
      121 catcgaggcg gcgggtcagt ggaccgcgac gaccttgcag aacgacttga acgcgtcgct
      181 gcgcgatgcc tccgatgcgt cgtccgcggc cgggccgctc gcttcgacgc gcacgccggt
      241 gaccggcgga ttggtggtca acggcggtgg caccggcgcg agctacacgc cgttcagcgg
      301 ctccgctgca ttcgacggcc tgttcgagat gaaccaggag tcgggcaccg tcacgcgggg
      361 ctcgtgggat ccgtctggtc cggtcatcgc gagcagcggc gggcgaggcg gacgcgaccg
      421 ggatcgtgac cgggatcgtg gacgcggtcg gtaccgcgac cgtgaccgcc cgcgcgatcg
      481 ccccaatccg tacgcgaccc ccgagccctc gacctgggtc ttgctcgggt caggactcct
      541 cgcgatcggc gctttcgccg gtttgcggcg ccgccaggcc ctgagcttct aaatatcccg
      601 gttatccaag gactggatac ccctcgggcc cgcccaccgg cgggcccgtt tcttttcggg
      661 atcgtccgcc cctgtcgccc gggttccccc tccgttggca tgcggcgtgc tccaacagct
      721 cgtttgaccc tgtcggggag gagccgactt tcgatgagca cgggaacgct gtatttcgtc
      781 ctacgcgaag gatccacgcg cgaggagcag tggagttggg aggagatcga aaacctctgc
      841 cgctccggcg acctcacacg gcgggcccgt atcttccttc ccgaagagaa ccgatgggct
      901 gaactgggcg agacgcgctt ggcggcggcc gccgccggct ccaagcccca ggaagcaccc
      961 gaggacgacg cggcctcgga ggcaaagaag aagctggagg ccgaatacca ggcggcgctc
     1021 gagcaggcgg aagccgcccc cgacgacctc gaagcccgtc tggacgcggg cgtgatcgcg
     1081 gcccagctcg gaaatcgcga cgaggcgaag aaccacttcc agtccgtgct ccatcgctac
     1141 ccgtaccacg cgcgcgcggc ccaggaagtc aaacggcgtt tctcgaagac ggagctccgg
     1201 accttccgct acctcgatcg cccggcgccg atttgggaag acctcggaca gctcgcggcg
     1261 atgccgctcg cgcgcgggcc gctgtacgtc atcgtgcccg ccgcggtgat cgcggggctg
     1321 tcgttcgtgc gcttcggcgg gattctcgcg tccgcgctcg tgttcttgtg gatgttccag
     1381 gtgatggagt acaccgcgcg cggcgcgaac aagccgccgg agtgggatcg cagcttcgcc
     1441 gacccgtggc gcaagctggg ccgtccatcg ctgctgatgg ccgtggtcgc gctccagctc
     1501 ggcgtcatcg tgttcgccgc ggccaagggc gtcatgctga tcgagggggt caaggaccag
     1561 acgctgtggc gctacatcgc gggcagcccg atcttcgtgg tcgccgcgtc catcgtgctc
     1621 gcgctgtatc ttcccgccgc gttcgtctca atcggcggct ttgcgggacc ggtggcgaag
     1681 acgctcgacc cgcgccgcct cgtcaagacg atcattcgca tggagcacga gtacatctac
     1741 tcggtggtga tcgtggctgc gatcgggatc gtggccgcga tcgtgtgcgg aatcaccgcg
     1801 ggcgctccag tggccggtga catggtgcgc ggcgcggtgc tcgcgtacgc gacaccggcg
     1861 gtagggttgg tgttggggcg gctcctcggc cgcgtcggac acgtgatcgc gtaataccgg
     1921 atcgcaacga gggaagcccc cgttgcgatc cggtgctcct acttgaccgc ggccatcaac
     1981 tcgcgcgcgg tgaacacggc ttccagcacg agaccttcct gcttcagcgc ctcacgcccg
     2041 ccttcttcgc ggtcgaccac ggccagcacc ttgcgcacct cgcaacccag cgccttcacc
     2101 gcggcgatcg cttgcagcga cgagccgccg gtcgtcacca cgtcttcgac gatgacgaca
     2161 cgctcgccct tcgacaacga tccctcgacc agtgattgcg tgccgtgccc cttcgcttcc
     2221 ttgcgaacga tgaagccctt gagcggcagg tcctcgaggc ccgcgagcgc gatcatcgcg
     2281 ccgacgatgg ggtcggcgcc cagcgtcaag ccgcccaccg cgtcgggcac gtcgtcggcg
     2341 accatggcga gaaaggtgcg ccccacgagg tacgcgccgc gcgcgtccag cgtcgtcatc
     2401 tttccgttga tgtagtagtt gctcttgcgc cccgacgcga gcgtgaactc gccggtgcga
     2461 aaagaatgcg cgagcaagtg ctgcagcagc tcgtcccgaa gcgtcatggt cttctcccgg
     2521 agtcttgatg gattcgacaa gcgatgggcg gatgcgccgt aagtcgcgcc agaataggcg
     2581 ttcgcgaccg ccgcggtcaa gggaatccct tgcgagcgcg gggggcatga gctatgtaat
     2641 gacgcgatgg cgagcatcct cgatggtatc gaacgcgcgc tggtcgcaat cgaccgcaca
     2701 ctggcgcagg gccggcgcgt gcctctgctg ctcttcatcg ccgcgctcgc actcaagatc
     2761 ggctacgtcg cccacaccgc cgacgcgctc tacgtcaagg tccccatcat ggacgcgcgc
     2821 tactacgaca ccatggcgca ggacatcgcg cgcggccacg tggtgcgcga cgaggccttc
     2881 ttcatgggac cgctgtaccc gtacttcctg gccctggtct actcgacggt gggacgcgac
     2941 ttcatgtggg tgcgcgtgct gcaggctgcg ggcggcgcgc tcaccgtgac cctcgcgttc
     3001 ctgatcggcc ggcgcgtgtt ccgcccgtcg gccgcgctcg tgggcgcggc gctcctcgca
     3061 ctgcacggcg ccatgacgtt ctacgagacc gagctcctga tggagtggat gggggcgctg
     3121 ctcaacgcgg ccgcgctgtt gctcctgttg cgcgcgcccg cggcgacgcc gcgcacctac
     3181 gccgcggcgg gagcaatcct cggcctctcg gcgctggcgc gggccagcat cctcgtgttc
     3241 gcggccgtgg tactggggtg gatcgtgcgc gtcgaatcgg ggcggcgccg cgtgcgcagt
     3301 gtgctcgcgt tcgggctcgc gatcgtggcc gtactcgcgc cggccgtcgt gcacaacctg
     3361 gcggtgagcc gcgacttcgt ccccgttacg tccaacgcgg gcatcaattt ctacatcgga
     3421 aacagcctgg ctgccaacgg caccttcgtg cccatccgcg gcgtcgacga gatcgacgac
     3481 gtgaccacca gcgagtacgt cgagcgcgcc accgggcgcg cggccacccc gagcgaggtg
     3541 tcgcactact ggttcgagcg cgcgatgacc gacgtacgcg ccgaccccgc gcgctctctc
     3601 cgattgatgg cgcgcaaggt gatcctgttc ttcaacggct acgaggttcc gcagatcgaa
     3661 agcttcgacg tgcacgccgc cgagtcgacc tggttgcgcg cgctgttcgt gagcacgtgg
     3721 ttcgtggtcg cggtcgggat attggggatg gtcctggcgg cgcgctcccg gcgcggcagc
     3781 gggctcttga gcgggtacgt gctcgcgtac gcggcgtcga tcgtggtgtt cttcgtgacc
     3841 gggcgctacc ggtcgcaggt cgtcccggtg ctgtgtctgt tcgcgggctt tgccttggtg
     3901 tcgctgccgg cgctcgcgcg acggccgcgc gcggcggccg cggccgcgtt cgccttgctg
     3961 ctgttgttcc tcgccaccaa cccgtcgatc tttcgcctcg acgagagcat ggtgatgtat
     4021 cgcgagtacg tgcaccgcgg gcggcgcttg agcgagctgc gaagctacgc gcccgcgctg
     4081 cgcgagatcg accgcgccat cgccatctat cccaagcagg ccgagggtta cgtgcagcga
     4141 gcggtcgtgc acaaggagaa cggcgacgat ttcaaggcca tcgaggacta cagccgcgcg
     4201 ctcgacatcg agcccgccgc accgtcggtc cactacgacc tcgcgcaagc gctgcggcgc
     4261 gtgaatctcc ccggcgaggc gatccgcgag taccagctcg cggtgcgcta cgaccccaac
     4321 atggtgcagg cgtacaacaa catgggggtt acgctgcgcg agatggggcg tttcgaggag
     4381 gcggtagccg cgtttcgcaa ggtgaccgag atcgcgccca actacgccaa ggcgtacaac
     4441 aatctgggcg cgtgctacgc cgagatgggt cggcccgagg acgcgatcgc ggtgttcgag
     4501 gagaccacgc ggcgctttcc cgagtacccg aacgggttca agaacctcgc catggcctac
     4561 gcgtcgcaga agcgcgtgcg gccggcgctg gcggccatgc gccgctacgc gctcctcgag
     4621 cccgccgacg ccgaggccaa ggagatcgtt cgcaagctgg agatcgcggc ggccgccgac
     4681 agcgcgtctt cgaactagcg caggtcaacc gcgagtccca gcgacacgcc gaacagcacc
     4741 gggtccaccg cctcggtggc ggtgaacacc cacaggagat cgctgtatcc cgcgctggta
     4801 tcgaagtagt gcagggtaca gttcgcttcc agcgcccaac cggtgcccaa gcgccgcatg
     4861 aggccgatgc tgccgttgcc tccgaagtag ctatcgctca catcgtcgga cacgaagtcg
     4921 gagacgaagg ccacgctcac cgaatagacg cccgccgcca gcgacgtcgt cagcgtgtag
     4981 ggactctttc cgagcgggaa tccgtaggcc agcccgaggc tgaaatagaa catgcgcatc
     5041 tccgactcca cgttggtgta gcgggtgatg aagtcgtcca actcagggtc caggatgtcg
     5101 cccaggtaga tcgcgccgac gtggaggtcg aggaagaggc gcgagtgaat gcgctcgttg
     5161 aagtagagca gcacgtccca gccgggaccg aagtactgat agacgccgtc gtaggtctcg
     5221 acgtaaccga tgcgtgcacc cagccgctct tgcgccgcga ggtcgtcgag cgcgcgagcg
     5281 ggtcgcgcgt gcgcacccac cagcacgacg agcacgagca gggccaagac gcgcgcgcgc
     5341 atgggtccaa tgtatgggga aacgcgctgg cggtaaagga cgattccgtc ctcgaagcgc
     5401 gtgcgtgcgg ggttacttca agagcgccat cttgcgcgtc tcgaccgcgc tgccgtcgac
     5461 cgtgagccgg tagaagtaca caccggacgc aagcgggttt tcgcgcccgt cggtgccgtt
     5521 ccacgcgacg cggtgtgcac ccgcgctctc gcgcccgtcc accaacgtcc gaatgtgcgc
     5581 gccgcgcgcg tcgaagatgt cgacgcgcac gtccgagtct cgcgcgagct cgtagccgat
     5641 ggtcgtcgcc gggttgaacg gattgggcgt gttgggcaag aggcgcgtcg tcgctcccgg
     5701 gcggggatcg gcggaggcct gggtgcgcga actggggact tgcttcccgc gaccggagcc
     5761 gcccgtcccc ggcagcagga cgtcgcccga ctcgacgatg tcgtcgacgc agatgtgtcc
     5821 gaagggactc gtcgacagat ccgcgatctc gatataaacc gatcttccga tgtgcggcgt
     5881 caggctccag tagcgacggt ccatttcgtt gctattgcga ccggattcct tgaacagcac
     5941 ttcggtggtt tgcgcgtcga ccagcgccac gtagcactgg ctcgggtagt cgccgccccc
     6001 caccagcaga ttcatcgaat tgccctcgat cgtgaatgga ttggagcgaa tgatccccat
     6061 cttggggtcg ccgaagtact gtcccggcgt cccgtagccc agcgggccgg tgtagtactc
     6121 gtaggagttg atccaaaaat caccctggaa cgtcgccgca tagttctcgt tacgcacgaa
     6181 cgcgttgtgg ccgaaggtgg gctggtagta gaaaccgtcg ccggatcccg ggacgaaggt
     6241 ccacgcgccg gagagcacca gcgggcgagg caccacggct tggtcgttga gccacaccat
     6301 cggggtgaat cgaaacacgt agcgctgggt gccgtgcgcg tcgttgtaca ccgcgtggcg
     6361 cgagaacagc tgcccgatat gggtgtcggt gatctgcggc gcgtggccga agtcgatgac
     6421 gcgcctcgtc ccgatatccc aacccgagag gagcgaatcc gaatacatgt gcgacgtgcc
     6481 cgcgacggtc tcctcggtga agaacatgtg ccatttgccg ttgggccgct gcaggatgaa
     6541 caccgattcg agcacgtgcc aactgtcgtg cacgtagatg gggccgatat cctcccagtc
     6601 gagcaagttg tcggagaccg cgcacgacac ggcaccgcgg ttggtgttgg tcttggcggt
     6661 attgaacatg taatagcggc cttggtactc cacgacgtgc gggtcgcgcc cgtgcgcgaa
     6721 gccggtctcg gtccactcgg cccatacggg gtcggggtga tagatcgggt ccggccactt
     6781 gtaccaatcg tacatggtcg tcgaccagga gatcccggcc tgctgcgagt tgagcaggtt
     6841 cacccccgtg taaaacatgg cccagccgcc ggtcgcgagc gggagcagga acggcgccca
     6901 catcgctttg ttgtcccacg tcccgagggt gagaacgggc ggctcgatgt cccaatggac
     6961 gaagtcggtc gtgatcgcgt ggccgatgtt gacggcgggg ttaccgcgca ggtagaagag
     7021 gtggtagacg ccgttgtcct cgaccacgca atggtcgaga accggctgcg gggcttcgct
     7081 gaaaagcgtg ccctcgaagt cgtagcgcac gaactcggcg ccggccggca cgggcgcaag
     7141 ggcccacgcg accgccacgg cggtggtgat cgttaccaag gatcgcaaag gatgacgttc
     7201 ggttgcgcgc atgaaacccg acgaccggcg gcgatggccg cggacgggga cagaaactat
     7261 gtcattcgaa cgacttacga aggggccggt tgcccagtca ttctagcaca cccgccgggc
     7321 aggggcaagt gacgtgaagt acaccgccgg catcggtcgc gtctttggca taccgctgaa
     7381 agtccacgcg accttcccct tgattctcgt ggtgtacgcc gcggtcgcgt ggagctccgg
     7441 ctcggccgcc gacgccttgc gcgcggtgat cctcgtgctc gcgatcttcg cttgtgtggt
     7501 gctccacgaa ctcggccact gcctccaagc gcgtcgctac ggcattcggt tccgcgacat
     7561 cctcttgctg ccgatcggtg gaatggcccg cgcggaatca ctccccgagc gccccgccac
     7621 gagatcctgg tggccatcgc gggaccggtc gtcagcttcg caattgccgc gattctcttc
     7681 gccgggctgg ctctcgcggg gacatccccg cacggcgacg gactcctggt cgacctggcg
     7741 tgggccaacc tcgcgctggg gctgttcaac ctgacgcccg cgttcccgat ggacggcgga
     7801 cgcatcctgc gcggcgccct ggcgacccgg atgtcctacc tggacgcgac gcgccgcgcg
     7861 cgcgccgtcg gccagctgct cgcactcggg ttcgcgaccc tcgcgttcct cgatttcacc
     7921 ttcgcgatgc tgtccgtgat cgcggtgctc gtcttcgtcg gcggcatgct ggaagagcgc
     7981 gcgattcgcg cgcgcgtggt gctctcgggc cggtgcgtcg gggatctcgt cgacgccgaa
     8041 acgcccgtgc tctcggccga cgaccgcgtc gtgtccgcca cgcgccgcgt gggcaccttg
     8101 gccgcgccgg cgtacgcggt cgcgggtgag gcgggctcgc tggccggggt ggtggccgcc
     8161 tccgacttga tgcgcgcgct gcgcgacggg cgcgcggacc aggatctctt gtcgatcgcg
     8221 cgccgcgatt tccccgtcgc ggaagcgagc acggacgcct cccgcgtcta tcgttacctg
     8281 cgcgagtcga acaaaccctt cgccgccgtc gtcgacggcg accgtttcgt ggggctcttc
     8341 cacgcggccg acgggtggta gggacgcccc ccaagcgccg cgcggggcgt cggggtgggg
     8401 catccttcct ttccgcggcg gtacacgatt tgctgcctct ctcccgcggt cgttccgacc
     8461 gccgatttcc gtgcaaaacg acgttccgaa ccgcgttcgc cgtgccggat cgcaacccca
     8521 ggagtgtgcc catgagcatt gtcgagacac tcgaggaaac cggcgcccct aacgggacgc
     8581 acgaatccaa cgttctcttc aaccgcgcgc tggaactggt aaatcgcaat cgagccgacc
     8641 aggctcgtaa gcacctggaa atggcgctgg ag
//
LOCUS       DASPGK010000449        10812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1397590, whole genome shotgun sequence.
ACCESSION   DASPGK010000449 DASPGK010000000
VERSION     DASPGK010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397590"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VEC56_12615"
     CDS             complement(<1..963)
                     /locus_tag="VEC56_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXV15041.1"
                     /translation="MRSLWNDADARAFASDPLALRVYASRLIGQDPSLVLHGGGNTSV
                     KAKARDLFGDEHDAIWVKGSGWDLATIERAGFAPLRLDVVRRMADHHSMTDARMVEML
                     RAALLDPNAPNPSVEAILHAVIPFTFVDHTHADAVVTITNTPKGVERIGEIYGNRIFI
                     VPYVMPGFILAREVFGMTKDIDWEEVEGIILMNHGVFTFGDDARTSYERMIQVVTEAE
                     DYLEMHAPLAQDDEEGADATTLTRGDLVTLARIRRAVSKAAQTPLLASHDGSPLHVAF
                     SSRADVGEIASRGPLTPDHVIRTKRAAFVAGADLDAGVERYAKEY"
     gene            complement(989..2740)
                     /locus_tag="VEC56_12620"
     CDS             complement(989..2740)
                     /locus_tag="VEC56_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV15042.1"
                     /translation="MKPAPWLLLILALPAAALAAPPQVVSVQPARQRVDAPANGVIEV
                     QFDADIDPSTVDAVSFRVFGRWSGPASGARQVVAGLITFIPDDPFFAGEYVTVSMSRA
                     IENTLGEAMAFGHAWSFWVETAPASLDLDFVTRYDVRQSGETWVQPYGAYAGDLDNDG
                     WSDLFVPCEQSDDVRIYMNNGAGLYPGGFTITKPTNMNTPSPNEGADFDNDGEIDVVV
                     TNVSSDRVIVMFGDGSGAFPTSVGLLAGATQVRGVGVLDLNGDGLDDIVTASRVLNKT
                     SIFINNGARTFAARVDIEAGVEAETSIAVADANNDGILDVFLGNYNIPRNVTVLLGNG
                     NGGLVAQTPVPTSGQPWMLAAGDLNGDGDVDVFSANSSGNGIGVHYGNGAGGISAVSS
                     LATGGFPLAIDAGDIDGDGDLELVSSDYGSGSWTVWENNGAGGFVDPRSLAASSAGSC
                     ATLHDRDNDGDLDLTGLDEVDDWVYLFANDSSAASAPRTPGAAVTMEQNHPNPFNPST
                     TIRFTLAGEVFVTLTVYDATGAFVVTLANARYAAGPHDVRWNGTDASGRRVASGLYFY
                     RLNAAGSELSRKMTLLK"
     gene            complement(2737..4029)
                     /locus_tag="VEC56_12625"
     CDS             complement(2737..4029)
                     /locus_tag="VEC56_12625"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXV15043.1"
                     /translation="MRASFASGHYIQSIRGRSMTRRFQTPESYQGSIISRVKRARATA
                     DPRKRDLAPTVLDFGPLRVALARHFGFCYGVENAVEIAYRTLAEHADRRVFFLSEMIH
                     NPSVNRDLVERGVRFIFTPAGEQLVPWEEITPDDVVVVPAFGTTLEMQEKLAERGVDA
                     YRYNTTCPFVEKVWKRSAQLGADRYSVVVHGKDTHEETRATFSHSRLGAPTVVVRNLA
                     EARQLGDVIRGTRGREDFRSTFGHKSSPGFDPERDLARVGVVNQTTMLATETHEIAQE
                     LRRALVDRYGPESIDEHFADTSDTLCYATIENQNATYALIEHGADLAIVVGGHNSSNT
                     SHIVELCEQAMPTYFVESADDLLSPSAMRHFSLAKRVVTTSEGWLPRQRPVTVVLTCG
                     ASCPDAILDGVLRRLLSMVPGVRAVDDVLAPFPEVEAV"
     gene            4032..4517
                     /locus_tag="VEC56_12630"
     CDS             4032..4517
                     /locus_tag="VEC56_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15044.1"
                     /translation="MNTRLLRGALALSLLANVWLAASHHRPVGEARADVAPPTEELAI
                     YMSHAQRHAHKLGLSIQAKNKPLAEFYLAELGETFELIQRKFPQYDGLQIAALSKAML
                     DPAKPALARSLAAGDFATASVAYDKYLAACNSCHVAAKHEYVKVVAPTGNPFNQSFAT
                     K"
     gene            complement(4492..5097)
                     /locus_tag="VEC56_12635"
     CDS             complement(4492..5097)
                     /locus_tag="VEC56_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV15045.1"
                     /translation="MVQTPSTMMPIGTRAPDFTLREPATGKTWSLADFRSPTLVVMFI
                     CNHCPFVIHVRDEITRLAREYGAKGVDLVAINANNMESHPQDGPEHMAALARELGWRF
                     PFLFDQTQETAKAYHAACTPDFFVFDRERELFYRGQLDDSRPSNGKPVTGADLRGALD
                     AAIAGRRPPVEQMPSIGCNIKWKPGNEPEYFTSFISSRKTG"
     gene            complement(5099..5578)
                     /locus_tag="VEC56_12640"
     CDS             complement(5099..5578)
                     /locus_tag="VEC56_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV15046.1"
                     /translation="MKSWVLAVLVTAVAASTVVPVHAGPKEELVAAVVQREAERQRAM
                     IEVDLAALDDLFAADATYIHSNGLAQTKAGVVAMLEKKELRYVSFDMKEATYRVYGET
                     IVCTGTQSIQLTSSGRPFTSNSRYTIVYAVIDGKPRVVAYQSTLLPEIVKQETTQGE"
     gene            complement(5628..6794)
                     /locus_tag="VEC56_12645"
     CDS             complement(5628..6794)
                     /locus_tag="VEC56_12645"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXV15047.1"
                     /translation="MRFPVVSGMPRRAWHVAETLVIACAFVACSSEDGGGPVDPPQPE
                     CPDPTPLTLTLPDNFPLLPNPADNPLTVEGVALGRRLFYDPILSADSSLSCGGCHLQS
                     LSFGDSRRFSEGIHGDKSRRQAPTIVNPGWNLRGIFWDGRAAGLEEQATQPVPEPTEM
                     SLPWPDAVAALQAHPEYPDLFCAAFGSSQVTMDRVVKAIAQFERTFVSANSKYDRWKR
                     GEEALTTMEERGFRLFMREGKGDCFHCHDQTLLATGTFHNTGLDTVAIDGGRGEITGN
                     PADYGKFKSPSLRNIMESSPYMHDGRFMTIREVLDHYNRGFRHGKPDTDPVDPQVDAL
                     INKRIQFPPMSSAELDTLEAFLETLTDWEFITNPDLSNPFETAAPVANRMRTAR"
     gene            complement(6812..7660)
                     /locus_tag="VEC56_12650"
     CDS             complement(6812..7660)
                     /locus_tag="VEC56_12650"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family protein"
                     /protein_id="HXV15048.1"
                     /translation="MIFTRFVLAAALGLAFLVACGDDDNPIDGDAPIELTAYFNLNVD
                     GQPIILNNLGYTNSSGTAYSIKALRFVITDVVMQYAGGSVKIADLHYYDLSDPSTQRF
                     EFTGLPHEEWTSVSFTFGLDETDNVRDKYLSRPQFHLDMQWPTTLGANLGYHYMQLEG
                     DYEVSPGTTAAYTTHMGPRQLDGTNPDFPGVEDATPHHFHFAVDLPFTPAHIHNEGRG
                     ELTINFNLNDWYADLDAGDGIDTEYDFADYPTQVIMGNLDAQGKLQHNGRFCFSAELE
                     SIGGHH"
     gene            complement(7845..8816)
                     /locus_tag="VEC56_12655"
     CDS             complement(7845..8816)
                     /locus_tag="VEC56_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15049.1"
                     /translation="MRRVRWAAAVAVALAVIAACDDDSVEPSGTAPLMRGLGGWTEFD
                     QWAVGDGGRIARYRGLGLTAVPSPTTNTLHDVVAFSASDVFMVGDNGTIIGPGLVEQA
                     SPTTHHLYSLFGYTASNLIAVGANGVIVEYDGGSWQTVSSPTSVDLHGVWGLEGGEVI
                     AVGVGGVVIRTDGVGPWAVVPSFTSNDLNAVWADKPGDWFAVGDAGEIWQERGTGWVP
                     MASPRGDDFHDVFGSDSAYVWAVGNADSILFYDQISWRPVAPNASGRIDAVWATICPL
                     SLAAGDPSGTAHCSNTYFAGAGGAFARYTLFNTFEELSAAASGEDAR"
     gene            complement(8818..9345)
                     /locus_tag="VEC56_12660"
     CDS             complement(8818..9345)
                     /locus_tag="VEC56_12660"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HXV15050.1"
                     /translation="MNINRILAVGSVVAIALSVPFFGPKWNRMMHLLGGVMFLGNVLV
                     TAVWASMARRNRDPEVVRFCVRGILLTDAVFTLPGVMLLFVNGGIIGTEWFKAGAPWL
                     MVSVILFTITGIVWGAVLVPLQKKLLRLVDAMPPRGPVPPEYDALFAKWFRWGGIATL
                     LPLVTFVLMVVRPVF"
     gene            complement(9388..9822)
                     /locus_tag="VEC56_12665"
     CDS             complement(9388..9822)
                     /locus_tag="VEC56_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV15051.1"
                     /translation="MTTVRDVLDRKGRNVFAVGPDAPVYRALELMSEKHVGALMVLDE
                     DRHLIGVVSERDYARKVALEGKSSKETPVRDIMTTKLFCVAPTHNIDECMSLMTDKSV
                     RHLPVLDGERLDGVISIGDVVKSMLSQQEFIIEQLEHFIAGR"
     gene            complement(9924..>10812)
                     /locus_tag="VEC56_12670"
     CDS             complement(9924..>10812)
                     /locus_tag="VEC56_12670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV15052.1"
                     /translation="TLGAVGAFFQPAIISVTPSLVPPERLAAANSLREASGELATLVG
                     QALGGVAFRLLGAPVLFLIDGVSYLFAAASTSLAKIPPHAGDANEGTRPSVRQALRQG
                     FAFLGETRGLKSLVVVATLANFFAAPFIVVLPFFVEDTLRAPSDWFGYLVAGIGAGGL
                     AGYALAGSIQRARAAGVLLACLCALSVCIGALALVSTRVQALALLVAVGVTGAVFNVG
                     VVTVLQRRTPDEFRGRVFGLAQTLAMATTPVAMLLAGLVADATGRDARLVFAGCGAAM
                     VVVTACAALHRPLRAYLSE"
BASE COUNT         1845 a         3582 c         3596 g         1789 t
ORIGIN      
        1 atactccttc gcgtagcgct cgacaccggc atccaggtcg gcgcccgcca cgaacgcggc
       61 gcgcttggtg cgaatcacgt ggtcgggtgt gagcggaccg cgcgacgcga tctcacccac
      121 gtcggcgcgc gacgagaacg cgacgtgcag cgggctgccg tcgtgggacg cgagcaaggg
      181 ggtctgggcc gccttcgaca ccgcgcgccg aatgcgcgcg agcgtcacga ggtcgccgcg
      241 ggtgagcgtg gtcgcgtcgg cgccttcttc gtcgtcttgg gccagcggcg cgtgcatctc
      301 gaggtaatcc tcggcctccg tcaccacctg aatcatgcgc tcatagctgg tgcgcgcgtc
      361 gtcgccgaag gtgaacacgc cgtggttcat gagaatgatg ccctcgactt cttcccagtc
      421 gatgtccttg gtcatgccga acacttcgcg ggccagaatg aacccgggca tcacgtaggg
      481 cacgatgaag atgcggttgc cgtagatctc gccgatgcgc tcgacgccct tgggcgtgtt
      541 ggtgatggtg accacggcgt cggcgtgggt gtggtcgacg aaggtgaacg gaatcacggc
      601 gtgcaggatc gcttcgacgg aggggttggg cgcgttgggg tcgaggagtg ccgcgcgcag
      661 catctccacc atgcgcgcgt ccgtcatgga atggtggtcc gccatgcggc gcacgacgtc
      721 gaggcgcagg ggcgcgaacc cggcccgctc gatggtggcg aggtcccatc cgctgccctt
      781 gacccagatg gcgtcgtgct cgtcgccgaa caggtcgcgc gccttcgcct tgaccgaggt
      841 gttgccgccg ccgtggagca cgagcgacgg atcctgcccg atcaggcgcg acgcgtacac
      901 gcgcagcgca agcgggtcgc tcgcgaacgc gcgggcgtcg gcgtcgttcc agaggcttct
      961 cacgcgggag actctagcgg cccgcgttct acttgagaag cgtcatcttg cgcgagagct
     1021 cggagccggc ggcgttgagg cggtagaagt agagacccga ggcgacgcgg cgaccggagg
     1081 cgtcggtccc gttccagcgc acgtcgtgcg ggcccgccgc gtagcgcgcg ttcgcgaggg
     1141 tgaccacgaa ggcgccggtc gcgtcgtaca cggtcagggt gacgaagacc tcgcccgcga
     1201 gcgtgaagcg aatggtcgtc gacgggttga acggattggg gtggttctgc tccatcgtca
     1261 cggcggcgcc cggggtgcgc ggcgcgctcg cggccgagga gtcgttcgcg aacagataga
     1321 cccagtcgtc cacttcgtcg aggccggtca ggtcgaggtc gccgtcgttg tcgcggtcgt
     1381 gcagggtcgc gcacgagccc gccgagctcg cggccagcga gcgcgggtcg acgaagccgc
     1441 cggcgccgtt gttctcccag accgtccacg accccgaccc gtagtcgctc gacaccagct
     1501 ccaggtcgcc atcgccgtcg atgtcgccgg cgtcgatcgc gagcggaaag ccgccggtgg
     1561 cgagcgacga caccgcggag attccgcccg cgccgttgcc gtagtggacg ccgatcccgt
     1621 tgccgctcga attggcggag aacacgtcga cgtcgccgtc gccgttcaag tcgccggccg
     1681 ccagcatcca gggctggccg ctggtcggca ccggcgtctg cgcgaccagg ccgccgttgc
     1741 cgttgccgag caggacggtg acgttgcgcg gaatgttgta gttacccagg aacacatcca
     1801 ggatgccgtc gttgttggcg tcggccaccg cgatcgacgt ctccgcttcc acacccgcct
     1861 cgatgtcgac gcgcgccgcg aaggtcctgg cgccgttgtt gatgaagatc gaggtcttgt
     1921 tgagcactcg gctcgcggtc acgatgtcgt ccaagccgtc gccgttcagg tcgagcacgc
     1981 ccacaccgcg tacttgtgtc gcccccgcca agagccccac gctggtgggg aacgcgcccg
     2041 agccgtcccc gaacatcacg atgacgcgat cgctgctgac gttcgtcacc accacgtcga
     2101 tctcgccgtc gttgtcgaaa tcggccccct cgttggggct gggcgtgttc atgttggtcg
     2161 gcttggtgat cgtgaagccg cccgggtaca ggccggcgcc gttgttcatg tagatgcgta
     2221 cgtcgtcgct ttgttcgcac ggcacgaaga gatccgacca gccgtcgttg tcgaggtcgc
     2281 ccgcgtacgc gccgtacggc tgcacccatg tttcccccga ctgacgcacg tcgtatcgcg
     2341 tcacgaaatc caagtcgagc gaggcggggg cggtctcgac ccaaaaactc catgcgtggc
     2401 cgaacgccat cgcttcgccc aaggtgttct cgatggctcg gctcatcgag acggtcacgt
     2461 attcgccggc gaaaaagggg tcgtcgggga tgaaggtgat cagccccgcg accacctggc
     2521 gcgcgccgct cgcgggcccc gaccagcggc cgaagacgcg gaagctcacc gcgtcgaccg
     2581 tggagggatc gatatcggcg tcgaactgga cctcgatcac gccgttcgcg ggcgcgtcga
     2641 cacgctggcg cgcgggttgg accgagacga cctgcggcgg cgcggcgagc gcggcggccg
     2701 ggagcgcgag tatcaggagc aaccacgggg cgggcttcat accgcttcga cctccgggaa
     2761 cggcgccagc acgtcgtcga cggcgcgaac gccgggcacc atcgagagca gccgccgcag
     2821 cacgccgtcg aggatcgcgt ccgggcacga cgcgccgcac gtcaagacca cggtgaccgg
     2881 acgctgccgt ggaagccagc cctccgacgt cgtcacgacg cgtttcgcca gcgagaaatg
     2941 acgcatcgcg gacggcgaga gcaggtcgtc ggcgctctcc acgaaatacg tcggcatggc
     3001 ctgttcgcac aattcgacga tgtgcgaggt gttcgacgag ttgtgcccgc ccaccacgat
     3061 ggcgagatcg gcgccgtgtt cgatcagcgc gtaggtggcg ttctggtttt cgatggtggc
     3121 gtagcacaac gtgtccgagg tatcggcgaa gtgttcgtcg atgctctcgg gaccgtagcg
     3181 atcgacgagc gcgcgccgga gctcctgcgc gatctcgtgg gtctcggtgg cgagcatcgt
     3241 ggtttgattc accacgccga cgcgcgccag gtcgcgctcg gggtcgaagc cgggcgagga
     3301 cttgtgcccg aaggtgctcc ggaagtcctc gcgcccgcgc gtgccgcgga tcacatcgcc
     3361 cagctgccgc gcctcggcga gatttcgcac caccacggtg ggggcgccga ggcggctgtg
     3421 cgagaacgtc gcgcgggtct cttcgtgcgt gtccttgccg tgcaccacga ccgaatagcg
     3481 atccgcaccg agttgcgcgc ttcgcttcca caccttctcg acgaacgggc aggtggtgtt
     3541 gtagcgatac gcgtccacgc cgcgctccgc cagcttctcc tgcatctcga gggtggtccc
     3601 gaaggccggc accaccacga cgtcgtcggg tgtgatttcc tcccacggca ccaattgctc
     3661 acccgcgggc gtgaagatga agcgcacgcc gcgctcgacc aggtcccgat tgacactcgg
     3721 attgtggatc atctcggaca gaaaaaacac gcgccggtcg gcgtgttcgg ccagcgtgcg
     3781 gtacgcgatc tcgaccgcgt tctcgacccc gtagcagaag ccgaagtggc gcgcgagcgc
     3841 cacccggagc gggccgaaat cgagcaccgt gggcgccagg tcgcgcttgc gcgggtcggc
     3901 cgtcgcccgc gcgcgcttga cccgcgagat gatggagccc tggtagctct ccggagtctg
     3961 gaaacgtctc gtcattgatc gtcctcggat gctctgaata taatgaccgc ttgcaaagga
     4021 ggcacgcaac catgaacacg cgtttactgc gcggcgcgct ggcgctctcg ctcctggcca
     4081 acgtctggtt ggcggcgtcg caccaccgtc ccgtcggcga ggcccgcgcc gacgtcgccc
     4141 cgccgaccga ggagctcgcc atctacatgt cgcacgccca gcgccacgcc cacaaactgg
     4201 gcctctcgat ccaggccaag aacaagcccc tggccgagtt ctatctggcc gaactcggcg
     4261 agacgttcga gctcattcaa aggaagtttc cgcagtacga cgggctccag atcgccgcgc
     4321 tctccaaggc gatgttggac cccgccaagc ccgcgctcgc gagatcgctc gcggccggcg
     4381 acttcgccac cgcctcagtc gcctacgaca agtacctcgc cgcctgcaac agctgccacg
     4441 tcgcggccaa gcatgagtac gtcaaggtgg tcgctcccac cgggaacccg ttcaaccagt
     4501 ctttcgcgac gaaatgaacg acgtgaagta ctccggctcg ttgcccggct tccacttgat
     4561 gttgcacccg atgctgggca tttgctcgac cggcgggcgg cgtcccgcga tcgcggcgtc
     4621 cagcgcgccg cgcaggtctg ctccggtcac cggctttccg ttcgagggcc ggctgtcgtc
     4681 gagttggccg cggtagaaca actcccgctc gcggtcgaac acgaagaaat cgggcgtgca
     4741 cgccgcgtgg tacgccttcg ccgtctcctg cgtctggtcg aacaagaacg ggaacctcca
     4801 gcccagctcg cgcgccaacg cggccatgtg ctcgggaccg tcctgggggt ggctctccat
     4861 gttgttcgcg ttgatcgcga ccaggtcgac gcccttggca ccgtactcgc gcgcgaggcg
     4921 cgtgatctcg tcgcgcacgt ggatgacgaa cgggcagtgg ttgcagatga acatgacgac
     4981 cagggtgggc gaacgaaagt cggccagcga ccaggtcttg cccgtggccg gctcgcgcag
     5041 cgtgaagtcg ggggcgcgcg tcccgatcgg catcatggtg gatggggttt ggaccatgtc
     5101 actctccttg ggtggtttcc tgctttacga tctcgggcaa gagcgtcgat tggtatgcga
     5161 ccacgcgcgg cttgccgtcg atcaccgcgt acacgatggt gtagcggctg ttggacgtga
     5221 acggccgccc cgacgaggtc agctgaatcg actgggtgcc ggtgcacacg atggtttcgc
     5281 cgtacacgcg atacgtagcc tctttcatgt cgaacgacac gtagcgcagt tccttcttct
     5341 cgagcatggc caccacgccc gccttggttt gcgcgagtcc gttcgaatgg atgtaggtcg
     5401 cgtcggcggc gaagaggtcg tcgagcgcgg cgagatcgac ctcgatcatg gcgcgctgcc
     5461 gctccgcctc gcgctgcacg accgcggcca cgagctcttc tttcgggccg gcatggacgg
     5521 ggaccacggt cgaggcagcg accgcggtca cgagtaccgc tagtacccag ctcttcatcc
     5581 gacgacctcc tcttcggcgc gcggccccac gccgtgcgac ccgttcatta tcgcgcggtt
     5641 cgcatccggt tggcaaccgg tgcggcggtc tcgaacggat tcgacaggtc gggattggta
     5701 atgaactccc agtcggtcag cgtctccaga aacgcctcca acgtgtcgag ctcggcggag
     5761 ctcatgggcg gaaactggat gcgcttgttg atcaacgcat cgacctgcgg atccacgggg
     5821 tccgtgtccg gcttgccgtg gcgaaatccg cggttgtagt gatcgagcac ctcgcggatg
     5881 gtcatgaagc gcccgtcgtg catgtagggc gacgattcca tgatattgcg cagcgacggg
     5941 gacttgaact tcccgtagtc ggccgggttg cccgtgatct cgccgcggcc gccgtcgatt
     6001 gcgaccgtgt ccagcccggt gttgtggaag gtaccggtcg cgagcagcgt ctggtcgtgg
     6061 cagtggaagc agtctccctt gccctcgcgc atgaagagtc ggaatccgcg ctcctccatc
     6121 gtggtgagcg cttcctcgcc gcgcttccag cggtcgtact tggaattggc cgacacgaag
     6181 gtgcgctcga actgtgcgat cgccttgacc actcgatcca tcgtcacttg cgacgacccg
     6241 aaggccgcgc agaacaggtc ggggtattcg gggtgcgctt gcagggccgc gaccgcgtcg
     6301 ggccagggca agctcatctc ggtcggttcc ggcaccggct gcgtggcctg ctcttccaaa
     6361 ccggccgcgc gaccgtccca gaaaataccg cgcaggttcc accccgggtt gacgatggtc
     6421 ggagcctggc ggcggctctt gtccccgtgg atgccctcgc tgaagcggcg cgagtctccg
     6481 aacgagagcg actgcaagtg acacccgccg cacgagagcg agctatcggc cgagagaatg
     6541 gggtcgtaga acaaccgccg cccgagtgcc acgccttcga ccgtaagcgg gttgtcggcc
     6601 ggattgggca agagcgggaa attgtccggc aaggtcagcg tgagcggcgt gggatcggga
     6661 cactccggct gcggcggatc gaccgggccg ccgccgtcct cgctcgagca cgcgacgaac
     6721 gcgcaggcga tgacgagcgt ctctgccacg tgccacgcgc gacgcggcat tcccgaaacc
     6781 actgggaatc tcatgtcacc caccgatgcg actaatggtg tccgccgatc gattccagtt
     6841 ccgcggagaa gcagaagcgg ccgttgtgct ggagcttccc ttgggcgtcg aggttgccca
     6901 tgatcacttg ggtcggatag tccgcgaagt cgtactcggt atcgatcccg tcgcccgcgt
     6961 cgaggtcggc gtaccagtcg ttcaggttga aattgatcgt caattcgccg cgaccttcgt
     7021 tgtggatgtg cgcgggcgtg aacggcagat cgaccgcgaa atggaagtgg tgcggggtcg
     7081 cgtcctccac ccccgggaaa tccggattgg tgccgtcgag ctggcgcggc cccatgtgcg
     7141 tcgtgtaggc ggcggtggtg ccggggctga cttcgtagtc gccctcgagc tgcatgtagt
     7201 ggtagccgag gttcgcaccc agcgtggtcg gccactgcat atcgagatgg aactgcggcc
     7261 tcgagaggta cttgtcgcgc acgttgtcgg tctcgtccag cccgaaggtg aacgagacgc
     7321 tggtccactc ttcgtgcggg agtccggtga attcaaacct ttgcgtcgac ggatccgaca
     7381 agtcatagta gtgcaaatcg gcgatcttga ccgatccgcc cgcatactgc atcacgacgt
     7441 ccgtgatgac gaagcgaagc gccttgatgc tgtacgccgt cccggaggaa ttcgtgtagc
     7501 ccaagttgtt gaggatgatc ggttgcccgt cgacgttgag attgaagtac gccgtgagtt
     7561 cgatcggcgc atccccgtcg atgggattgt cgtcgtcgcc gcacgcgacg agaaaagcca
     7621 aacccagtgc cgcggccaag acgaaacgcg tgaatatcat cgatcctccg agacgagaaa
     7681 ccggggcggt ggagcggact tcgggagcgt gtgacgatcc cgctcgcgac gtacttgatt
     7741 atacggtagc ggcgcgactc gcggcaagcc gagttccagc cctcagcgca ggtcgtccgc
     7801 gatgggcagc gacggctcga tgcgccgcat cgttccgtcc cgtttcagcg tgcgtcttcg
     7861 ccggacgcgg cggcggagag ctcctcgaac gtgttgaaca gggtatagcg cgcaaaggct
     7921 ccgcccgcac ccgcgaagta ggtgttcgag caatgcgcgg tgcccgacgg atcgccggcc
     7981 gcgagcgaga gcggacaaat cgtggcccat acggcgtcga tgcgcccgct cgcgttgggc
     8041 gcgaccggtc gccacgagat ctggtcgtag aagaggatcg agtccgcgtt tccgaccgcc
     8101 catacatacg cgctgtcgct gccgaacacg tcgtggaagt cgtcgccgcg cggcgaggcc
     8161 atcggcaccc agcccgtgcc gcgctcctgc catatttctc ccgcgtcgcc gaccgcgaac
     8221 cagtcaccgg gcttgtcggc ccacaccgcg ttcaggtcgt tcgacgtaaa ggagggcacg
     8281 accgcccacg gccccacgcc gtcggtccga atcacgacac cgccgacgcc caccgcgatc
     8341 acctcgccgc cctcgagacc ccacacaccg tgcagatcga cggacgttgg actcgacacg
     8401 gtctgccagc ttccaccgtc gtactcgacg atgacgccgt tggcacccac cgcaatcaag
     8461 ttggaagcgg tataaccgaa aagcgaatag agatgatggg tggtcggact cgcctgctcg
     8521 accaggcccg gcccaatgat ggtcccgtta tctccgacca tgaacacgtc ggaggcggag
     8581 aatgcaacca cgtcgtgcag cgtgttcgtg gtcggcgagg gcacggcggt cagcccgagc
     8641 ccgcggtagc gcgcaatccg cccgccatcg cccaccgccc actggtcgaa ctcggtccag
     8701 cctccgagac cgcgcatcaa gggcgccgtg ccggacggct ccacgctgtc atcatcgcag
     8761 gcggcgatga cggcgagcgc caccgcgacc gccgcagccc atcgaacgcg gcgcatgcta
     8821 gaagacgggc cgcacgacca tgagcacgaa cgttaccagc gggagcagcg tggcgatgcc
     8881 gccccagcga aaccacttgg cgaagagagc gtcgtactcg ggtggcacgg gaccgcgcgg
     8941 cggcatcgcg tcgaccaaac gaagcagctt tttctggagc ggcaccagca cggcgcccca
     9001 cacgatgccg gtgatggtga acaggatcac cgagaccatg agccacgggg cccccgcctt
     9061 gaaccactcg gttccgatga tgccgccgtt cacgaagagg agcatgacgc cggggagcgt
     9121 gaagacggcg tcggtcagca ggatgccgcg cacgcagaag cgaaccacct cggggtcgcg
     9181 gttgcggcgg gccatactcg cccacaccgc ggtgacgagg acgttgccga ggaacattac
     9241 gccgccgagg aggtgcatca tccggttcca cttgggaccg aagaacggca ccgacaacgc
     9301 gatggcgacg accgagccga cagcgagaat acgattgatg ttcatccggg catgctagcg
     9361 ccggacaccg agccgatcaa ccgtttttca gcggccggca atgaagtgct cgagctgctc
     9421 gatgatgaac tcctgctgcg acagcatcga cttgaccacg tcgccgatcg agatgacgcc
     9481 gtccagccgc tccccgtcga gcacgggaag gtgcctcaca ctcttgtcgg tcattagact
     9541 catgcactcg tcgatgttgt gcgtgggcgc cacgcagaac agcttcgttg tcatgatgtc
     9601 gcgtaccggc gtctctttcg acgactttcc ttccagggcg accttgcgcg catagtcgcg
     9661 ctcggatacg acaccgatca gatgccgatc ctcgtcgagc accatcaacg cacccacgtg
     9721 cttctccgac atcaactcga gggcccggta caccggggca tcggggccga ctgcgaacac
     9781 gttgcggccc ttgcggtcga gcacgtcgcg aaccgtcgtc atggcatacc tcccatgttg
     9841 gaccattcca gcgtagaccc gggcgcggtc accgtcaacc gtgtttatct cttagttact
     9901 atattcacga ggcgtcggta ccctcattcg gacaggtagg cgcgcagcgg gcggtgcagg
     9961 gccgcgcacg cggtgacgac aaccatcgcc gcgccgcacc cggcgaagac caagcgcgcg
    10021 tcgcggccgg ttgcatcggc gacaaggccc gcgagcagca tcgcgaccgg tgtggtcgcc
    10081 atggccagcg tctgtgccaa gccgaacacg cggccgcgaa actcgtcggg cgtgcggcgt
    10141 tgcagcaccg tcacgacgcc cacgttgaac acggcgcccg tcacaccgac ggcgacgagc
    10201 aacgcgagcg cctgcacccg cgtcgacacc agcgccagcg cgccgatgca tacagacagc
    10261 gcgcacagac aggcgagcaa gaccccggct gcgcgagcgc gctgaatact ccccgcgagt
    10321 gcgtaccccg cgagcccccc cgcgccgatg ccggccacga ggtagccgaa ccagtcgctc
    10381 ggcgcacgca gggtatcttc gacgaagaac ggcaacacca cgatgaaggg cgcggcgaag
    10441 aaattggcga gcgtggccac cacgacgagc gacttcagtc cccgtgtttc gccgaggaac
    10501 gcgaacccct gccggagcgc ctgtcgcacg ctcgggcgcg tgccctcgtt ggcgtcgccg
    10561 gcgtggggcg gaatcttcgc caacgacgtc gatgccgcgg cgaacaggta cgagacaccg
    10621 tcgatcagaa agaggaccgg ggcgcccagc aggcgaaacg cgacgccgcc cagggcttgc
    10681 cccaccaagg tggccaattc ccccgacgcc tcgcgcagcg aattggcggc cgcgaggcgt
    10741 tcgggcggca ccagcgacgg cgtgaccgat atgatcgcgg gctggaagaa cgcaccaacc
    10801 gcacccagag tc
//
LOCUS       DASPGK010000450         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_814690, whole genome shotgun sequence.
ACCESSION   DASPGK010000450 DASPGK010000000
VERSION     DASPGK010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814690"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VEC56_12675"
     CDS             complement(<1..1171)
                     /locus_tag="VEC56_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV15053.1"
                     /translation="MPTPFVNEPLSNFDDPAVGSKMQQAIDRVRSELGRDYPLVIDGQ
                     KVKTPATIASIDPSHPDTVVGRVAEGKREHAERALQAALERFKTWQHVAAEERASYLF
                     KAAKVIRDRKFDYAAWMVFEVGKSWAEADADVAETIDFLEFYGREALRLAAEQPVTKI
                     KGEDNALVYIPLGAGVVIPPWNFPMAIMAGMTSAAIVAGNTVVLKPSSDSPVIAAKFV
                     EVLEEVGLPAGVVNFVPGGGGEVGGYLVQHPKTRFIAFTGSKEVGLHINQEAATPRKG
                     QVWIKRAVLEMGGKDSIIVDEGVDLDEAAKGVVASAFGFQGQKCSACSRAIVHEKVYD
                     AFVARLVPLVNAIKQGAPHESKDNAMGPVVNEKAAHDILGYIETGKSEGKLLTGGM"
     gene            1307..1708
                     /locus_tag="VEC56_12680"
     CDS             1307..1708
                     /locus_tag="VEC56_12680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV15054.1"
                     /translation="MRVHVETIAVRYAETDRMGVAHHSSYLLWFEIGRTGLLRAIGHP
                     YRELEGSGILLPVVEYGVRFSKAADYDDRLAIETTVREVKSRVATFDYRARRGDDVIA
                     EGFTRHVCTDIHRRARRFPDLLVEAIAPFLR"
     gene            complement(1806..2096)
                     /locus_tag="VEC56_12685"
     CDS             complement(1806..2096)
                     /locus_tag="VEC56_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXV15055.1"
                     /translation="MSASHDHVALHAWISGRVQGVFFRDATRREAIALGLSGWVRNLR
                     DGRVEALFVGPRAACEKALAFVRVGPPRAHVDGVEYAWETPRSDASHAFEIR"
     gene            complement(2093..>2739)
                     /gene="nadE"
                     /locus_tag="VEC56_12690"
     CDS             complement(2093..>2739)
                     /gene="nadE"
                     /locus_tag="VEC56_12690"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HXV15056.1"
                     /translation="LDDALSLAQNLGVRIEVVPIQEAMEAYDRVLAGLFPGRVKDVTE
                     ENIQARIRGNVLMAISNKFGHLVLSTGNKSELAVGYCTLYGDMSGGLAVISDVPKTTV
                     YALARRINEGRSVIPEYILARAPSAELRPDQKDTDTLPPYDVLDPILDAYIEDRLSIS
                     EIVARGFDRAVVEWVVAAVHKNEYKRKQAAPGIKVTSKAFGVGRRFPVAARFTL"
BASE COUNT          438 a          947 c          871 g          483 t
ORIGIN      
        1 gcatcccgcc cgtcaggagc ttgccctcgg acttgccggt ttcgatgtaa cccagaatgt
       61 cgtgggccgc cttctcgttc acgaccggac ccatggcgtt gtccttggac tcgtggggcg
      121 cgccctgctt gattgcgttc accaggggga ccaggcgcgc gacgaaggcg tcgtacactt
      181 tctcgtgcac gatggcgcgc gagcacgcgg agcacttctg cccctggaat ccgaacgcgg
      241 acgcgaccac gcccttggcc gcctcgtcca gatccacgcc ttcgtccacg atgatcgaat
      301 ccttgccgcc catctccagg accgcgcgct tgatccacac ctggcctttg cggggtgtgg
      361 ccgcctcttg gttgatgtgc agccccactt ccttgctgcc cgtgaacgcg atgaagcgcg
      421 tcttggggtg ctggaccaag tagccgccta cttcgccgcc gccgcccggc acgaaattca
      481 cgacaccggc cggaagcccg acctcttcta gaacctccac gaacttggcc gcgatcacgg
      541 gcgagtcgct ggacggtttc aagacgacgg tattgcccgc cacgatggcg gccgacgtca
      601 tgccggccat gatcgccatc gggaaattcc agggcggaat cacgacaccg gcgccgagcg
      661 ggatgtacac gagcgcgttg tcctcgccct tgattttcgt caccggctgc tcggccgcca
      721 ggcgcagcgc ctcgcgtccg tagaactcga ggaaatcgat ggtctcggcg acgtcggcgt
      781 ccgcttcggc ccagctcttg ccgacttcga acaccatcca cgccgcatag tcgaacttgc
      841 ggtcgcgaat gaccttcgcg gccttgaaca ggtacgacgc gcgttcttcg gcggcgacgt
      901 gctgccaggt cttgaagcgc tcgagggccg cttgcaacgc acgctcggcg tgctcgcgct
      961 tgccttccgc gacccggccg accacggtat cggggtggga cggatcgatg gacgcgatag
     1021 tcgccggcgt cttcaccttc tgcccgtcga tgaccaaggg atagtcgcgg ccgagctcgg
     1081 agcgcacgcg gtcgatcgcc tgctgcatct tggacccgac cgcgggatcg tcgaaattgg
     1141 acaggggctc attcacgaat ggcgtgggca tcgaaactcc tcggtattgg aatgacggca
     1201 atcgatgtct ggaagcggcg ccctctacta taagaaaccg gtcgcgtgct gtaaacgggc
     1261 tttctcgctt gcgcgaccac gcgcaccttg gctaccgtgg tggcccatgc gcgtccacgt
     1321 cgaaaccatc gcggtccgct acgccgagac cgatcgcatg ggtgtggccc accactcgag
     1381 ctatctcttg tggttcgaga tcggccgcac cggcctgttg cgcgcgatcg gccaccctta
     1441 ccgcgagctg gaagggagcg ggatcctgct accggtggtc gagtacggcg tgcgcttctc
     1501 gaaggccgcc gactacgacg accgtctggc catcgagacc acggtgcgcg aggtcaagag
     1561 tcgcgtggcg acgttcgatt accgcgcgcg tcgcggcgac gacgtcatcg cggagggttt
     1621 cacgcgccac gtgtgcaccg acattcatcg ccgcgcccgc cgtttccccg acctactggt
     1681 cgaagcgatc gcaccgtttc tccggtaatt ctcgaacgct gcccggcggg tcctgtcact
     1741 cgtcaccccg ccgcctggcg cggcgggggc cccacgagcg ccacccgccg ggggatgctc
     1801 ggaggctagc gaatctcgaa cgcgtgcgaa gcgtcggagc gcggggtctc ccacgcgtac
     1861 tcgacgccgt cgacgtgcgc gcgcggcggg ccgacgcgca cgaatgcgag ggccttctcg
     1921 cacgcggcgc gcggtcccac gaatagggct tcgacgcgtc cgtcgcggag attgcgaacc
     1981 caccccgaga gcccgagcgc gatcgcctcg cggcgggtgg cgtcgcgaaa gaaaacaccc
     2041 tgcacgcgcc cgctgatcca cgcgtgcagc gcgacgtggt cgtgcgacgc actcacagcg
     2101 tgaagcgcgc ggccaccgga aaacgccgcc ccacaccgaa cgccttcgac gtcaccttga
     2161 tcccgggtgc ggcctgcttg cgcttgtatt cgttcttgtg cacggccgcc accacccact
     2221 ccacgaccgc gcggtcgaag ccgcgcgcca cgatctcgga gatcgaaagc cggtcttcga
     2281 tgtaggcgtc gaggatgggg tcgagcacgt cgtacggcgg cagcgtgtcg gtgtccttct
     2341 ggtcggggcg cagctccgcg gagggcgcgc gcgccagtat gtactcggga atcacgctcc
     2401 gcccctcgtt gattcggcgc gcgagcgcgt acaccgtcgt cttgggtacg tcggagatga
     2461 cggccagtcc gccgctcatg tccccgtaca gcgtgcagta cccgaccgcc aattcgctct
     2521 tgttccccgt cgagagaacc aggtgaccga acttgttcga gatcgccatc agcacattgc
     2581 cgcggatgcg ggcctggata ttctcctcgg tgacgtcctt cacacgaccc gggaagagcc
     2641 cggcgagtac gcgatcgtac gcctccatgg cctcctgaat cggcaccacc tcgatgcgca
     2701 cgccgaggtt ttgcgcgaga gagagcgcgt cgtccaggc
//
LOCUS       DASPGK010000451         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_932408, whole genome shotgun sequence.
ACCESSION   DASPGK010000451 DASPGK010000000
VERSION     DASPGK010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932408"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /gene="glmS"
                     /locus_tag="VEC56_12695"
     CDS             <1..1174
                     /gene="glmS"
                     /locus_tag="VEC56_12695"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXV15057.1"
                     /translation="FVTKTIDNVTTNKEVHQVDWDLEQIEKGGFDHFMLKEINEQPET
                     ISNGMRGRLVYDEGIAKLGGLNMTDDELRSTKRIMILGCGTSWHAGLIGEYIIEEHAR
                     IPVEVEYASEFRYRNPIIEPGTVTLAISQSGETIDTLEAMREARRRGSRILGISNVVG
                     STIARESEGGVYIHAGPEIGVASTKAFTSQITVLSLLALLLGRLRVMSKREGQEQIEH
                     LQRLPSQVQQILDQDEAIKEIAQAYCNDTNFLYLGRGANFPVALEGALKLKEISYIHA
                     EGYPAAEMKHGPIALIDKNMPVVVICPKDNAYHKILGNIAEVKARRGRIIVICNEGDD
                     EVAEMADHVITIPQTLDFLYPILTVLPLQLLAYHIAVLRGCDVDQPRNLAKSVTVE"
     gene            1188..1583
                     /locus_tag="VEC56_12700"
     CDS             1188..1583
                     /locus_tag="VEC56_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV15058.1"
                     /translation="MKSPLHSVHAPKPIGPYSQAIRAGDFVFLSGQIGIDPEAGKITA
                     KDAVGQTTQALKNIRAILTAAGLSAEHVVKTTIFLADMNDFAAVNEAYGEFFHDDPPA
                     RSTVQVARLPMDAKVEIEAIAMRRPASSA"
     gene            complement(1584..2690)
                     /locus_tag="VEC56_12705"
     CDS             complement(1584..2690)
                     /locus_tag="VEC56_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HXV15059.1"
                     /translation="MDRFVLRGRVRHRAALAAFSTSFVLVNTALAAPTRVYVFGAKPN
                     ATFEVRKNSGFVATVTATPFGTVSADADAVSGDRVEVTAPDLAPPVPPLFASLATNDA
                     GCATAAWLPSGDPSVMGYVVSFGTQSVAGGGAPAYEQSVDAGTSTLHTECSLTPGTYY
                     FAVRARNAGGLSAYSIERSVVIQTLAVLFSMFEATAGEDGVRLSWRVEADEVVQGYRV
                     YRSEGAAPEVRIADLNAGAESYVDASARAGSSFTYIVAAVNEAGDEVRSVPASVTTPT
                     LALSLGQNYPNPFNPTTKIPFTLESEGRVLIRVFDVRGARVATIFDGTLGQGRHSVEW
                     SGKDDAGRPVASGLYLYTLTTGKRTMSKKMVMVK"
     gene            3023..3481
                     /locus_tag="VEC56_12710"
     CDS             3023..3481
                     /locus_tag="VEC56_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15060.1"
                     /translation="MFTWLGKNPLKSWLRPPSTPTRAVEIELRADGEAAWTPATIVSD
                     RPMVQLVAELVAVNASDAKVLLVSARLRKPATEGLVRVHDPDGGTAATRVLEPFASAR
                     ASVFFFVPLAPPPAGTNVVADVGVLDQFGNTHWLEKVRFRGRALDECDYF"
     gene            3538..>4056
                     /locus_tag="VEC56_12715"
     CDS             3538..>4056
                     /locus_tag="VEC56_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15061.1"
                     /translation="MPHRLGSIVLALLLPIASPASAAPPNVDRADVPTGILYDLVVPL
                     AHLERFDGSAGSPPANAATLRQAVFELARASLDPTAWPDAHAFRDDGGPAVHIGLVDL
                     RYDRIRETARRSGAARVEGDRLVLEPGALETARAFLAAPVRGYTYRGADLAFVLDRGL
                     FVGDAASLTRVEI"
BASE COUNT          718 a         1344 c         1349 g          645 t
ORIGIN      
        1 cttcgtcacc aagaccatcg acaacgtgac gaccaacaag gaagtccacc aggtggactg
       61 ggatctagag cagatcgaga agggcggctt cgatcacttc atgctgaagg agatcaacga
      121 gcagcccgag actattagca acggcatgcg cggccgcttg gtctacgacg aaggcatcgc
      181 caagctggga ggtctcaaca tgaccgacga cgagctccgt tcgaccaagc gcatcatgat
      241 cctgggctgc ggcacgagct ggcacgcggg cctgattggc gagtacatca tcgaggagca
      301 cgcgcgcatc ccagtcgaag tggaatacgc ctccgagttc cgttaccgca atcccatcat
      361 cgagccgggg acagtgacct tggcgatcag ccagagcggc gagaccatcg acacgttgga
      421 agcgatgcgc gaagcgcggc gcagaggctc gcgcatcctc ggcatctcga acgtagtggg
      481 ctccaccatc gcgcgcgagt ccgaaggcgg cgtgtacatc cacgcgggcc cggaaatcgg
      541 cgtggcgtcg accaaggcgt tcacgtcgca gatcaccgtg ctctcgctgc tcgcactctt
      601 gctgggccga ctgcgcgtca tgtcgaagcg cgaaggccag gagcagatcg agcatctcca
      661 gaggctcccg agccaggtcc agcagatcct cgaccaggac gaggccatca aggagatcgc
      721 gcaggcctac tgcaacgaca ccaacttcct gtacctcggc cgcggcgcga atttcccggt
      781 cgccctcgag ggcgccttga agctcaagga gatttcgtac atccacgcgg aaggctaccc
      841 cgccgccgag atgaagcacg ggcccatcgc gctcatcgac aagaacatgc cggtagttgt
      901 catctgcccg aaggacaacg cgtaccacaa gatcctgggt aatattgcgg aagtgaaggc
      961 gcgccgcggt cgcatcatcg tgatatgcaa cgagggcgac gatgaagtcg cggagatggc
     1021 ggatcacgtc atcacgatcc cgcagacgct cgacttcctg tatccgattc taacggtgtt
     1081 gccgctgcag ctgctcgcgt atcacattgc cgtgctgcgc ggctgcgacg tcgatcagcc
     1141 gaggaatctc gccaagagtg tgacggtgga ataaaggagt aacgagaatg aagtcccccc
     1201 tgcattccgt tcatgcgccc aagcccatcg gtccctacag ccaagccatc cgcgccggag
     1261 acttcgtgtt tctctccggg cagatcggga tcgaccccga ggcggggaag atcacggcca
     1321 aggacgccgt cggccagacc acgcaagcgc tcaagaacat ccgcgctatt ctgaccgccg
     1381 ctggtttgag cgccgagcac gtggtcaaga ccaccatctt cctcgccgac atgaacgact
     1441 tcgccgccgt caacgaagcc tacggcgagt tcttccacga cgacccgccc gcccgctcca
     1501 cggtgcaggt ggcgcgactc cccatggacg ccaaggtcga gatcgaggcg atcgcgatgc
     1561 ggcgaccggc ctcgagcgcg tagctacttc accatcacca tcttcttcga catcgtccgt
     1621 ttgccggtcg tgagcgtgta gaggtacaag cccgacgcga ccggccggcc ggcgtcgtct
     1681 ttgcccgacc actcgaccga gtggcggcct tgtcccagcg tcccgtcgaa gatcgtcgcg
     1741 acccgcgctc cgcgcacgtc gaagacgcga atcagcacgc gcccctcgct ctccaaggtg
     1801 aacgggatct tggtggtcgg attgaacgga ttggggtagt tctgcccgag tgagagcgcg
     1861 agcgtcggtg tcgttaccga agccggaacc gatcgcactt cgtcgccggc ttcgttcacc
     1921 gccgccacga tgtaggtgaa ggaacttcct gcgcgcgcgc tcgcgtcgac gtaggattcc
     1981 gcgccggcgt ttaggtcggc gatgcgaacc tcgggggcgg cgccttcgct tcgatacacg
     2041 cggtagcctt gcaccacttc gtccgcctcc acgcgccacg agagccgcac gccgtcttcg
     2101 ccggcggttg cttcgaacat cgagaacaac accgccagag tttggatcac gaccgagcgc
     2161 tcgatcgagt acgcgctcaa cccgccggcg ttgcgcgccc gcacggcgaa gtagtaggtg
     2221 ccgggcgtga gcgagcactc ggtgtggagt gtggaggtcc cggcgtcgac cgactgctcg
     2281 tacgcaggag cccctccgcc tgcgaccgac tgggtcccga acgagaccac gtagcccatc
     2341 accgaagggt cgccgctggg gagccaggcc gcggtcgcgc acccggcgtc attggtggcg
     2401 agggaggcga acaacggcgg gacgggcggc gccaggtcgg gtgccgtgac ctccacgcga
     2461 tcgccggaga cggcgtcggc gtcggcggag acggtcccga acggggtcgc ggtaacggtc
     2521 gcgacgaagc ccgagttctt gcgaacctcg aaagtggcgt tgggcttggc gccgaacacg
     2581 tacacgcgtg tgggcgccgc gagcgcggta ttgacgagga cgaaggaggt cgagaaggcg
     2641 gcgagggcgg cacgatggcg cacccgtccc cgaaggacga atcggtccat gggcgtactc
     2701 ctggcggggc gctcgcttgg gaggcatgcg gcggccacgc gctggtgcga gtccagagca
     2761 tccggacgac gatttatggg gcggtgcgct cggcggctcg gccgggcgcg ggcagggggc
     2821 aactcactcg gaacgaagcg cgcgggggga gggtggcgga gtgcgcgcag acgtccgcgt
     2881 acagtggcgc caatatcacc cggaccgacg acgcgtggcg atcaaataag atgcttgagt
     2941 gtgagcggga aaacagggga ctatcagtta gtcattgggg atctcggcgc ccctgcggga
     3001 gtatgcgcgc ggagtggcaa ccatgttcac atggctcggt aagaatccct tgaagtcgtg
     3061 gctgcgtccg cccagcacgc caacgcgcgc ggtcgagatc gagctgcgtg ccgacggcga
     3121 ggcggcgtgg actcccgcca ccatcgtgag tgaccgaccg atggtgcagt tggtggccga
     3181 gctcgtcgct gtcaacgcga gcgacgccaa ggtgctgctc gtgtccgcgc ggctccgcaa
     3241 gccggccacc gaaggtctgg tccgcgtgca cgatcccgac ggcggtacgg ccgcgacccg
     3301 cgtgctcgag ccgttcgcgt cggcgcgcgc gtcggtcttc ttcttcgtcc cattggcgcc
     3361 gccgcccgcg ggcaccaacg tggtcgccga cgtcggagtc ctcgatcagt tcggcaacac
     3421 ccactggctc gaaaaggtgc gtttccgcgg tcgcgcgctc gacgagtgcg actacttcta
     3481 gagggcgcgc ggcggagtcg tggtatcatc ggggctctct ctccaggagc ctgaaccatg
     3541 ccacaccgcc ttgggtcgat cgttctcgcg ctgctgctcc ccatcgcatc cccggcgtcc
     3601 gccgcgccac cgaacgtcga tcgcgcggac gtccccaccg gcattctcta cgacctcgtc
     3661 gtcccgctcg cgcacctgga gcgcttcgac ggctcggcgg gatcgccgcc ggccaacgcg
     3721 gcgaccttgc gccaggccgt cttcgagctc gcgcgcgcat cgctcgatcc gaccgcgtgg
     3781 ccggacgcgc acgcgtttcg cgacgatggc gggcccgccg tgcacatcgg cctcgtcgac
     3841 cttcgctacg accgcattcg cgaaacggcc cgccgctcgg gggcggcccg ggtcgaaggc
     3901 gaccgcctcg tgctcgagcc gggcgccctc gaaacggcgc gcgcgttcct ggccgcaccg
     3961 gtgcgtggat acacctaccg cggggccgac ctcgcgttcg tcctggaccg agggctcttc
     4021 gtcggcgacg cggcgagttt gacgcgggtg gagatc
//
LOCUS       DASPGK010000452         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1517547, whole genome shotgun sequence.
ACCESSION   DASPGK010000452 DASPGK010000000
VERSION     DASPGK010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517547"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1850)
                     /locus_tag="VEC56_12720"
     CDS             complement(117..1850)
                     /locus_tag="VEC56_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV15062.1"
                     /translation="MRSGTLVVAGLMAALALGACSQMGGKAELGPAEQARAQELFDAL
                     TAAHKASNWDAAASAASELVNQYPRFARMDEALLLAGQTADARERYAEAAAYYDRLSA
                     DYKLSSSRAPALRAAAADYAKLDDPVREASSWLELMKTPLDAASRDEISRRLRHLVDE
                     ELSQGDLDALYHDYPDSPLARGALYKQARMAYAAGDYDRSYELVGRYLDALPRGESDP
                     DARRLLELATERRQAPPPGPTSRVRPDRVGLLLPQTGSLALYGRLFEQGAKLAVDEFN
                     KQHSRRVSLAVADSRGGAIDAVMAVRRLTSEEGAVAIVGDVFTLPAIAGAIEANAWRA
                     PIVSPVVASDELVQIGPWVFQTRVPGTVEATAVAEAAVTKLSLERVAVIAPSRGDRRD
                     AADFFSEEVKRLGRQVVAIEYFEDGATDFRPQLERIREAAPDALFAVGSVEELLQMLP
                     QAKFHDVQVQMLGLSQWNSDKLMRLARAELEGAVFPAESHYGSTRKDLADAVAKLATP
                     NTTDASPVSIAGYYGTRAVLQAIGEGAASREDVRAYLEKHLRGDAETRAARAAAVPLV
                     RVRAGRVEPFE"
     gene            complement(1850..2344)
                     /locus_tag="VEC56_12725"
     CDS             complement(1850..2344)
                     /locus_tag="VEC56_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HXV15063.1"
                     /translation="MALNEKHFVRVAVSGMARDARENPVVLLKSSEGEEVLPIWIGHA
                     EGLAIELTMKGEKFERPLTHDLLKTAIEGLGAVLAKVAITELRNNTFFAKLYLQRDQE
                     LIVVDARPSDSVALALKCDAPIFVARDVFASHKRSLGQESGPGPDSDDELRRYLKDLD
                     PGDF"
     gene            complement(2357..>3069)
                     /locus_tag="VEC56_12730"
     CDS             complement(2357..>3069)
                     /locus_tag="VEC56_12730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV15064.1"
                     /translation="RVVEGGEKFTTMAHLYSEDTITKADGGDLGYFNPGGYIRGVGYS
                     KEFTDAVSQMEPGQIYGPVKWEKGYSLVRVNEVQPAVLRPYDEVREEIAGRLARDKIE
                     NVRTQHFAEVETHYDTRNYLQEAYEKMQRGPQELFNYAQNSTDPRQRIAAFQQIVDKF
                     PQDAYAPQAMFMIGFVYAEELKDYLTADRTFNELIAKYPESEMAQTARWMLDNLEKPL
                     PKFEDLDDLNRQIEEKSN"
BASE COUNT          453 a         1047 c         1028 g          541 t
ORIGIN      
        1 cggactccgc gctcgacgcc gtgcgtcagt tcgtgttcga gccgccgacc gagaacggcc
       61 gcccgaccac gatctgggtg cggctgcgga tcaaattccg cattaacgcc tagccgctat
      121 tcgaaaggct cgacgcgacc cgcgcgcacg cgcaccagtg gaaccgcggc cgcgcgcgcc
      181 gctcgcgtct ccgcgtcgcc gcgcaggtgc ttctccaagt aggcgcgtac gtcctcgcgc
      241 gacgcggctc cctcaccgat cgcttgcagt accgcgcggg tgccatagta acccgctatc
      301 gacaccggcg acgcatcggt cgtattggga gtggcgagct tcgcgaccgc gtcggccaga
      361 tctttccgcg tcgacccgta gtgcgattcc gcggggaaca ccgcgccctc cagctcggcg
      421 cgcgccaggc gcatgagctt gtccgagttc cactggctca agcccagcat ctggacttgg
      481 acgtcgtgaa acttggcctg cgggagcatc tgcaagagct cctccacgct tcccaccgcg
      541 aacaacgcgt cgggcgccgc ttcgcgaatg cgctcgagct gcggacgaaa gtcggtggcg
      601 ccgtcctcga agtactcgat cgcgaccacc tgccgtccca gccgcttgac ctcctcggag
      661 aagaagtcgg ccgcgtcgcg gcggtcgccg cgcgagggcg cgatcacggc cacgcgctcg
      721 agcgagagct tggtcacggc cgcctcggcg accgcggtcg cttccaccgt ccccggcacg
      781 cgggtctgga acacccaggg cccaatctgc accaattcgt cgctggccac caccggagag
      841 acgatgggcg cgcgccacgc gttggcttcg atagcgcccg cgatggcggg cagcgtgaac
      901 acgtcgccga cgatcgcgac cgcgccctcc tcgctggtca ggcgacgcac cgccatcacc
      961 gcgtcgatgg cgccgccgcg cgagtccgcg accgcgagcg acacgcgccg cgagtgttgc
     1021 ttgttgaatt cgtccacggc cagcttcgcg ccctgctcga acaaacggcc gtagagcgcg
     1081 agcgagcccg tttgcggcag caggaggccg acgcggtcgg gccgcacgcg cgaggtgggc
     1141 ccgggcgggg gcgcctggcg tcgctccgtc gcaagctcga gcagacggcg cgcgtcgggg
     1201 tcgctctcgc cgcgcggaag cgcgtcgagg tatcgcccca ccagttcgta ggaacggtcg
     1261 tagtcgccgg ccgcgtaggc catgcgcgcc tgcttgtaga gcgctccgcg agcgagcgga
     1321 gagtcgggat agtcgtggta gagggcgtcg agatcgccct ggctcaattc ttcgtcgacc
     1381 agatggcgca ggcgccgcga gatctcgtcg cggctggcgg catcgagtgg ggtcttcatg
     1441 agctcgagcc acgacgaggc ctcgcgtacc gggtcgtcga gcttggcgta gtcggcggca
     1501 gcggcgcgca aggccggtgc acgcgagctg ctgagcttgt agtcggcgga gaggcggtcg
     1561 taataggcgg ccgcttccgc gtagcgctcg cgcgcgtccg cggtctgccc cgcgagcagc
     1621 agcgcttcgt ccatgcgcgc gaaccgcggg tactgattga cgagttccga ggccgcgctg
     1681 gcggcggcgt cccaattcga cgccttgtgg gccgcggtga gcgcatcgaa gagctcctgc
     1741 gcgcgggcct gctcggcggg gccgagttcg gccttgcctc ccatctgcga gcaggcgccc
     1801 agggcgagcg ccgccatcaa tcccgcaacg acgagggtac cagagcgcat cagaaatctc
     1861 ccgggtcgag gtctttcaag tagcgtcgca actcgtcgtc cgagtcgggt ccggggccgc
     1921 tctcctggcc gagcgagcgc ttgtgcgacg cgaacacgtc gcgggcaacg aagatgggtg
     1981 cgtcgcactt gagtgcgagc gccacggaat cgctggggcg ggcgtccacc acgatcagct
     2041 cctggtcgcg ctgcaggtac aacttggcga agaaggtgtt gttgcgcagt tcggtgatgg
     2101 ccaccttggc gagcacggcg cccagccctt cgatcgccgt tttcagcagg tcgtgggtga
     2161 gcgggcgctc gaacttctcc cccttcatgg tgagctcgat cgcgaggcct tcggcgtgcc
     2221 cgatccagat cggcagcact tcctcgccct cactgctctt gagcagcacc acggggtttt
     2281 cgcgcgcgtc gcgggccata ccgctcaccg cgacacgcac gaagtgcttt tcgttaagcg
     2341 ccacgcgccc tccgggctag ttcgactttt cttcgatctg tcggttcagg tcgtccagat
     2401 cctcgaactt gggcagcggc ttctccaggt tgtcgagcat ccagcgcgcc gtctgcgcca
     2461 tctcgctctc gggatacttc gcgatcagct cgttgaaggt ccggtccgcg gtcagatagt
     2521 ccttcagctc ctcggcgtac acgaagccga tcatgaacat ggcctgggga gcgtacgcgt
     2581 cctggggaaa tttgtcgacg atctgttgga aggccgcgat gcgctgcctc gggtcggtgc
     2641 tgttctgcgc gtagttgaac aactcctgcg gcccgcgctg catcttctcg taggcctcct
     2701 gcaagtagtt gcgcgtgtcg tagtgcgtct cgacttcggc gaagtgctgc gtgcgcacgt
     2761 tttcgatctt gtcgcgcgcg aggcgacccg cgatctcttc gcgcacttcg tcgtaggggc
     2821 gaagcacggc cggctgcact tcgttgacgc gcaccagcga gtaacccttc tcccacttga
     2881 cggggccgta gatctgcccc ggctccattt gcgagaccgc gtcggtgaac tccttcgagt
     2941 agcccacgcc gcggatgtac ccgcccgggt tgaagtaccc caggtcgccg ccatcggcct
     3001 tggtgatggt gtcttcggaa tacaggtgcg ccatggtggt gaacttctcg ccgccctcga
     3061 ccacgcgat
//
LOCUS       DASPGK010000453         7652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_229996, whole genome shotgun sequence.
ACCESSION   DASPGK010000453 DASPGK010000000
VERSION     DASPGK010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229996"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="VEC56_12735"
     CDS             <1..1861
                     /locus_tag="VEC56_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15065.1"
                     /translation="VVIETRDRFRSEVVLEARVMLRRRDYTVNPYDGSILFMEPVPVT
                     DRELNPNTIAVTYETESDGADAYLFGVRGDVVEGKRYRAGVTAVANSGDGPGYALYGA
                     DGEVGWRSLRIGGEIARSEDDVAGAGDAYKVGASASHGSSKLDLYLRRVDGDFSNPSF
                     RGADREHASVKAGFDGRWAVNPQVAVHADGFTHEFQQTDELKESARATVDYRRRLLEM
                     SAGLRVARHDDPTAGGDPDARGVLALAGLTVGNRGSFGVSTTLEQNVAGDIVDDYPNR
                     LKTVLAAPLSERFRAIATYEYATASGLPSTHQVTAGVEGTSSHGTQAYTRYALDRAAS
                     DARMGAVSGIRQQLRLNGTTSATVGVEGFLSMAGRNDDEYVSLTTGLASKRLGSHFVE
                     GGYEYRWEDTGHKHLVRLTGVQQLDGGFAWLTKNVLGVGTHELQPGASRNEAQYYATL
                     AGTYRSPHAPVQSLAMIKSYYDRYAPVEPDAIRWRLVASIDVNAFPNPQHEVRFKYAY
                     KHVEDWSDAEALTTDMDLVLGQYVWRFAPGWDLDAWGRVLRLRDGGSQEYGTGLEIGR
                     LMFRSVRVGVGYSLNGFDDPDVSTTDAWSSGFGVRLQIILSDWLLADFEGLEK"
     gene            1864..5826
                     /locus_tag="VEC56_12740"
     CDS             1864..5826
                     /locus_tag="VEC56_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15066.1"
                     /translation="MSRRFIIGFALALAALSASSARAGELIVNGGFEAGGFGTSWVHG
                     ARQGNTQNPALADHVVALDLPYAGNYSALLGFKYVTQGTNRYGYMYQDVAIPAGVSAA
                     TLNFKIRMQGYDSDFYDPFYVDVRTTGNAQLERVLFYAFSEWNNIYKDSGWLFDDNVL
                     PVGHNMTAYAGQTIRLYFEQANLIDALYETWTYVDDVSLVYRMWVDLAVDGNGDDVFG
                     ALGSGNGGTSIKSGVAGDTLSYDVRVENEGTVADTYRLTATAPIGWSAFVDLGGVLTP
                     LPYVTPSMALGSSTTYRIVVIPPVAAGNGSYSVILNAQSTVQASRVDSARLRADIVSA
                     VLGSDLTVNGNGVGVIGDNGAGGFALTASPWDTTVPYTVVVRNTGNAASAFSVAWTND
                     PNLTASVVYNATTYNAPFTTASIPAGGTATMTLNLRVASPNPGGDYTNILTATSTGDT
                     TKRDSIKSILRLLAPRVDMIVATSGDNIYGATGTGLGGGSSNAGERGTQVQFPIIVQN
                     ESALADSFTLTWTAPAAGWTAVIEIGGVNRNFPVITPRINAFSQASYTLRVSIPGGAA
                     YGTYRSVMNAASRVDNRITESISASISVATPSEIDMTIDGQGLDVYGPIGTGLGGTSI
                     RTVSPGQTTVFSVVIQNVSGVNNFSVGWSAPAGWTVTFNGSATPIASIAAGTYPLSVT
                     VPASSLGGTFDVIVDGRKVDKPFLMDSVTGRVVVIPPAIVDGVIDGNGNDVFGVLGSG
                     AGGASLQVTPAPATLNFTVEIQNQGPTADVYQVTWGAIPAWTALLNGLPSPFTTGSVP
                     AGGSRVYPFSVTVPANEATGSFQYIIDMVSINDPTSFESIAARVDIVGPPRPDLVIDG
                     NGFDVFGPLGSGQGGTSTRSAAPSTVYTSALRVRNAGAFADSMRVQWDVPSGWPLHSV
                     VIGDTAVTYQAPFYTPLLDPGQYVDYIVTVTVPAGANAAHTTIINSWSSRPPNLSESV
                     ALVTETRGLVRGWVFDDRDHDGVLTAGDVALGGVLVSETSLGFSVTTAGDGSFAMQVA
                     GDSITVVEQNPPGFVSLSPDTAGPFVIAAGDTVDVFFADIGGLTITSGTSQPGPTGAF
                     VAFAHRVVAGTAGHVDLSATTTDSLAVLAWYFDANANGLLDGPDRPLFPSDGDLDPDG
                     ANAGVLHVLLRVSVPAGATPGSTIVIAVGAQQAVTNTSLVLTANATDVVVVVDGSAGR
                     LTMQKTSDRADAAPGDVIEYSVRLFNAGADSLANVILVDPVSSWVDLEANAFGAGLDL
                     EWRPPVGAPVHFTFDPTDPDECEYDTPTRTLRVVLSKNTTFYLAPGEAGVVVYRVRVR
                     "
     gene            5986..7587
                     /locus_tag="VEC56_12745"
     CDS             5986..7587
                     /locus_tag="VEC56_12745"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HXV15067.1"
                     /translation="MSHSEFAPKVGLEAVGLTNLGGVLWNPTPATLYEEFVMNGEGVI
                     ANHGPIVAETGKYTGRSPDDKFVVEEASSKDKIWWGKVNRAFDEEKFNRLMHRVQAYL
                     QDGMVYVQDCWAGADHDQRVPIRVVTQIAWHSLFARNMFIPVLPEERKNHAPQFTVIS
                     APGFKADPAIDGTRSEAFIIVHFGRGIVLIGGTSYAGEIKKSIFTVMNYLLPQRGVLS
                     MHCSANEGPSGDVAVFFGLSGTGKTTLSADPKRSLIGDDEHGWSDHGVFNFEGGCYAK
                     AIHLSPEAEPEIHATTRRFGTVLENVVIDEHTREIDLHDNALTENTRASYPITQIPNA
                     KLDGVGGHPRNIVMLTCDAFGVLPPIARLTPEQASYHFLSGYTAKVAGTERGMGKEPQ
                     ATFSTCFGAPFLALPPTVYSDLLAKKMRHHDTKCWLVNTGWSGGGYGVGARMKIAYSR
                     AMVNAALEGKLDRVKYAKDPIFGLDVPAECPGVPAEVLSPRNTWSDKAAYDKKARELV
                     ALFEKNFAQFEQAVSAEVRAAGMSA"
BASE COUNT         1279 a         2580 c         2576 g         1217 t
ORIGIN      
        1 cgtcgtgatc gagacgcgcg accgtttccg gtccgaggtg gtgctggagg cgcgcgtcat
       61 gctccggcgc cgcgactaca ccgtcaaccc gtacgacgga tcgatcctgt tcatggagcc
      121 ggtgccggtc accgaccgcg agctcaaccc gaacacgatc gcggtcacct acgagaccga
      181 gagcgacggc gccgacgcgt acctgttcgg cgtgcgcggc gacgtggtcg agggcaagcg
      241 ctaccgcgcc ggcgtcaccg cggtcgccaa ctcgggtgat ggccccggct acgcactcta
      301 cggcgccgac ggcgaagtgg gctggcgttc gctgcgcatc gggggcgaga ttgcgcgcag
      361 cgaggacgac gtcgcgggcg cgggcgacgc gtacaaggtc ggcgcgtccg cgtcgcacgg
      421 ctcgtccaaa ctggatctgt acctgcggcg cgtcgacggc gacttctcca acccatcgtt
      481 ccgcggcgcc gaccgcgagc acgcgagcgt caaggccggc ttcgacggcc gctgggccgt
      541 caacccgcag gtcgcggtgc acgccgacgg cttcacgcac gagttccagc agaccgacga
      601 gctcaaggaa tccgcgcgcg cgaccgtgga ctaccggcgc cgcttgctcg agatgtcggc
      661 cggcttgcgg gtggcgcgcc acgacgaccc cacggccggc ggagaccccg acgcgcgcgg
      721 cgtgctggca ctcgcggggc tgacggtcgg caaccgcggc agcttcggcg tgtccaccac
      781 actcgagcag aacgtggccg gcgacatcgt cgacgactat ccgaatcgct tgaagacggt
      841 gctggcggct ccgctctccg agcgcttccg cgccatcgcg acctacgaat acgcgaccgc
      901 gagcggactg ccgtcgacgc accaagtgac ggccggcgtc gaggggacgt cgtcgcacgg
      961 cacccaagcg tacacgcgct acgcgctcga ccgcgccgcg agcgacgcgc gcatgggcgc
     1021 ggtgagcggc attcgccagc aactgcgctt gaacggaacc acctccgcga ccgtgggcgt
     1081 cgaagggttc ctgtccatgg cggggcgcaa cgacgacgag tacgtgtccc tgaccaccgg
     1141 cctcgcgtcg aagcggctcg ggtcgcactt cgtcgaaggc ggctacgagt accgctggga
     1201 ggacaccggt cacaagcacc tcgtgcgtct cacgggcgtg cagcagctgg acggcgggtt
     1261 cgcgtggctc accaagaacg tgctcggtgt gggaacccac gagctccaac cgggtgcgag
     1321 ccggaacgag gcgcagtact acgccaccct ggcgggcacg tatcgctcgc cgcacgcgcc
     1381 cgtacagtcg ctcgcgatga tcaagagcta ctacgaccgc tacgcaccgg tcgagccgga
     1441 cgcgattcgc tggcgcttgg tggcgtcgat cgacgtcaac gcattcccga atccgcagca
     1501 cgaggtgcgc ttcaagtacg cgtacaagca cgtcgaggac tggagcgacg cggaggcact
     1561 caccaccgac atggacttgg tgttgggcca gtacgtgtgg cgcttcgcgc ccggctggga
     1621 cctggacgcg tggggccgcg tgctgcggct gcgcgacggc ggctcccagg aatacggcac
     1681 tggccttgag atcgggcgcc tcatgttccg ctcggtgcgc gtgggcgtcg gctacagcct
     1741 gaacggcttc gacgaccccg acgtcagcac gacggatgcg tggagcagcg gcttcggcgt
     1801 gcgcttgcag atcattctat ccgactggct gttggccgac ttcgaaggat tggagaagta
     1861 gcgatgtcgc gcaggttcat catcgggttc gcgctcgcgc tcgccgcttt gtcggcgtcg
     1921 tccgcgcgcg cgggcgaatt gatcgtcaac ggcggcttcg aagccggggg cttcggcacg
     1981 tcgtgggtgc acggtgcgcg ccaaggcaac acccagaacc ccgccttggc cgaccacgtg
     2041 gtggcgctcg atctcccgta cgccggaaac tactcggcgc tgctcggctt caagtacgtc
     2101 acgcaaggga cgaatcgcta cggctacatg tatcaagacg tcgccatacc ggcgggtgtc
     2161 tcggccgcga ccctgaactt caagattcgc atgcaggggt acgacagcga cttctacgac
     2221 cccttctacg tcgacgtgcg caccaccggc aacgcccagc tcgagcgcgt gctgttctac
     2281 gcgttcagcg agtggaacaa catctacaag gacagcggct ggctctttga cgacaacgtg
     2341 ctgccggtcg gacacaacat gacggcgtac gcggggcaga ccatccgttt gtacttcgag
     2401 caggccaacc tgatcgacgc cctctacgag acctggacct acgtcgacga cgtgtcgctg
     2461 gtctatcgca tgtgggtcga cctggcggtg gacggcaacg gcgacgacgt gttcggcgcg
     2521 ctgggcagcg gcaacggcgg cacctcgatc aagagcgggg tcgcgggcga cacgctctcg
     2581 tacgacgtgc gcgtcgagaa cgaaggcacg gtggcggata cctaccggct caccgcgacc
     2641 gcgcccatcg ggtggtcggc cttcgtcgac ctgggcggcg tgctgactcc gcttccgtac
     2701 gtcacgccct cgatggcgct gggatcgtcg accacgtatc gcatcgtcgt gattccgccc
     2761 gtcgcggcgg gcaacgggtc ctacagcgtg attctcaacg cgcagagcac ggtacaggcg
     2821 agccgcgtcg acagcgcgcg cctgcgcgcg gacatcgtga gcgcggtcct cggctcggac
     2881 ctcacggtca acggcaacgg cgtgggtgtg atcggcgaca acggcgcggg tggtttcgcg
     2941 ctgacggcct cgccgtggga tacgacggtg ccgtacacgg tcgtggtacg caacaccggc
     3001 aacgcggcgt cggccttctc ggtggcgtgg accaacgacc ccaacttgac cgcgtcggtg
     3061 gtgtacaacg cgaccacgta caacgcgccc ttcacgaccg cgagcattcc cgccggcggt
     3121 actgccacca tgacgctcaa cttgcgcgtc gcttcgccca accccggtgg ggactacacc
     3181 aacatcctga ccgcaacctc gaccggcgac accaccaagc gcgactcgat caagtcgatc
     3241 ctgcgcttgc tggcgccgcg cgtcgatatg atcgtcgcga ccagcggcga caacatctac
     3301 ggcgcgaccg gcaccggcct gggcggcggc agctccaacg cgggcgagcg cggcacccag
     3361 gtccagttcc cgattatcgt ccagaacgag agcgccctgg ccgactcgtt cacgctgacc
     3421 tggaccgcgc ccgccgcggg ctggaccgcg gtcatcgaga tcggcggcgt gaatcgcaac
     3481 ttcccggtga tcacgccgcg catcaatgcg ttctcgcaag cgagttacac gctccgggtg
     3541 agcataccgg gaggcgccgc ctacggcacg tatcggtcgg tgatgaacgc ggcctcgcgc
     3601 gtggacaacc gcatcaccga gagcatttcc gcgagcatca gtgtcgcgac gccgagcgag
     3661 atcgacatga ccatcgacgg gcagggactc gacgtatacg ggcccatcgg aaccggcttg
     3721 ggaggaacgt cgattcgcac cgtgtcgccg ggacagacca cggtgttcag cgtcgtcatc
     3781 cagaacgtgt cgggggtcaa caacttcagc gtgggctgga gcgcgcccgc cggttggacg
     3841 gtcaccttca acgggagcgc gacacccatc gcgagcatcg cggccggcac ctatcccttg
     3901 agcgtcaccg tgcccgcgtc gtccttgggc ggcaccttcg acgtcatcgt cgacgggcgc
     3961 aaggtcgaca agcccttcct gatggacagc gtcacaggcc gcgtggtcgt gataccgccc
     4021 gcgatcgtcg acggcgtgat cgacggcaac ggcaacgacg tgttcggggt gctcggcagc
     4081 ggagcgggcg gggcttccct gcaagtgacg cccgcgccgg cgacgctgaa cttcacggtg
     4141 gagatccaga accagggacc gaccgctgac gtgtatcaag tgacgtgggg tgcgattccg
     4201 gcctggaccg cgctgctcaa cgggctgccc tcgccgttca ccaccggctc ggtgccggcg
     4261 ggcgggtcgc gcgtgtatcc gttcagcgtg acggtgccgg cgaacgaggc gaccggcagc
     4321 ttccagtaca tcatcgacat ggtgtcgatc aacgatccca cgtcgttcga aagcatcgct
     4381 gcgcgcgtcg acatagtggg tccgccgcgc cccgacttgg tcatcgacgg caacggcttc
     4441 gacgtcttcg gcccgctggg ttcggggcag ggcggcacct cgacgcgttc cgcggccccg
     4501 tcgaccgtgt acacctccgc gctgcgcgtg cgtaacgcgg gcgccttcgc cgattcgatg
     4561 cgcgtgcagt gggacgtgcc gagcggctgg ccgttgcaca gcgtcgtcat cggcgacacc
     4621 gcggtcacgt accaagcgcc gttctacacg ccgctgctcg accccggcca gtacgtcgac
     4681 tacatcgtga cggttacggt gccggcgggc gccaacgcgg cccacaccac catcatcaac
     4741 agctggtcgt cgcggccgcc gaacctctcc gagagcgtgg cactcgtgac cgagacgcgc
     4801 ggcctggtgc gcggctgggt gttcgacgac cgtgaccacg acggcgtatt gaccgccgga
     4861 gacgttgcgc tcggtggcgt gttggtctcg gagacgtcgc tgggattctc ggtcacgacc
     4921 gcgggcgacg gatcgttcgc gatgcaggtc gcgggcgaca gcataaccgt cgtcgagcag
     4981 aatccgcccg gcttcgtgtc gctctcgccc gacacggcgg ggccattcgt gatagcggcc
     5041 ggcgacacgg tcgacgtttt cttcgccgac atcggcggtc tcaccatcac gtcggggacg
     5101 tcgcagccgg ggccgaccgg ggctttcgtc gcgttcgcgc accgcgtcgt cgcgggcacg
     5161 gccgggcacg tcgatctgtc ggcgaccacg accgattcgc tcgcggtgct ggcgtggtac
     5221 ttcgacgcca acgccaacgg tctgctcgac ggccccgacc gcccgctctt cccgagcgac
     5281 ggcgacctcg atcccgacgg cgccaacgcg ggcgtgctcc acgtcctctt gcgcgtgtcg
     5341 gtcccggccg gcgccacacc gggctcgacc atcgtgatcg cggtgggcgc gcagcaggcg
     5401 gtgacgaaca cgtcgctcgt cttgaccgcg aatgccaccg acgtggtggt ggtcgtcgac
     5461 ggatcggcgg ggcgattgac gatgcagaag acgtcggatc gcgccgacgc cgcgcccggc
     5521 gacgtgatcg agtactcggt gcggctgttc aacgccggcg ccgacagcct ggccaacgtg
     5581 atactggtcg atccagtctc ctcgtgggtc gatctggaag cgaatgcgtt cggggccggg
     5641 ctcgatctcg agtggcgtcc gcccgtcggc gcgcccgtgc acttcacgtt cgacccgacg
     5701 gatccggacg agtgcgaata tgacacgccg acgcgcacgc tgcgcgtcgt gctctcgaag
     5761 aacacgacct tctatctggc gccgggcgaa gcgggtgtgg tcgtctaccg cgtgcgcgtg
     5821 cgctgagagc catcgccccg ttcgaccggg ggtctagaat cccctggaat cccacgcctc
     5881 gaggcgctac actctccggc gctttccatc ctatcgatcg aatgcaattc gaaccccggc
     5941 cgcatgccga gggtttcgct ttttctgatt aacggagggt tttcgatgtc gcacagcgag
     6001 tttgcgccga aggtcggctt ggaggcggta ggcctcacca acctcggcgg cgtgctctgg
     6061 aatcccaccc cagccacgct ctacgaagag ttcgtgatga acggcgaggg cgtcatcgcg
     6121 aatcacgggc ccatcgtcgc cgagaccggc aagtacaccg gccgcagccc ggatgacaag
     6181 ttcgtcgtcg aagaggcgtc gagcaaggac aagatctggt ggggcaaggt caaccgcgcg
     6241 ttcgacgagg agaagttcaa ccgcctcatg caccgcgtgc aggcgtatct ccaggacggc
     6301 atggtttacg tgcaggactg ctgggcgggc gccgaccacg accagcgcgt tcccattcgc
     6361 gtggtcacgc agatcgcgtg gcactcgctg ttcgctcgca acatgttcat cccggtgctc
     6421 cccgaagagc gaaagaacca cgcgccccag ttcaccgtta ttagtgcccc cggcttcaag
     6481 gccgacccgg ccatcgacgg aacccgcagc gaagcgttca tcatcgtcca ctttggtcgc
     6541 ggcatcgtct tgatcggcgg cacgtcgtac gcgggcgaga tcaagaaatc gatcttcacg
     6601 gtcatgaact acctgctccc gcagcggggc gtgctgtcga tgcactgcag cgccaacgag
     6661 ggcccgagcg gcgacgtcgc ggtgttcttc ggcctttcgg ggacgggaaa gaccacgctg
     6721 tcggccgacc ccaagcgcag cttgatcggc gacgacgagc acggctggtc cgaccacggc
     6781 gtgttcaact tcgaaggggg gtgctacgcc aaggcgatcc acctctcgcc ggaagcggag
     6841 ccggagatcc acgccaccac gcgccgcttc gggacggtcc tggaaaatgt cgtgatcgac
     6901 gagcacacgc gcgagatcga tctgcacgac aacgcgctga ccgagaacac gcgcgcgtcg
     6961 tatcccatca cgcagattcc caacgccaag ctcgacggcg tgggcggtca cccgcgcaac
     7021 atcgtgatgc tcacgtgcga cgccttcggc gtgctgcccc cgatcgcgcg gctcacaccc
     7081 gagcaggcca gctaccattt cctgtcgggg tacaccgcga aagtggcggg caccgagcgc
     7141 gggatgggca aggagccgca ggcgaccttc tcgacctgct tcggcgcgcc gtttctcgcg
     7201 ctgccgccca ccgtgtattc ggacctgctg gccaagaaga tgcgccacca cgacaccaag
     7261 tgctggctcg tcaacacggg ctggagcggc ggcggctacg gtgtgggcgc gcgcatgaag
     7321 atcgcgtact cgcgcgcgat ggtgaacgcg gcgctggaag ggaagctgga ccgcgtgaag
     7381 tacgccaagg atcccatctt cggtctcgac gttcctgccg agtgcccggg ggttcccgcg
     7441 gaggtcttga gcccgcgcaa cacctggagc gacaaggccg cctacgacaa gaaggcgcgc
     7501 gagctggtgg cgctgttcga gaagaacttc gcgcagttcg agcaggcggt gtcggcggag
     7561 gtacgggcgg cagggatgag cgcgtaacgc ggctcgcggt cgtgcacaag aaaaggggtg
     7621 gtgagcgtgc tcaccacccc tttggtttgg tt
//
LOCUS       DASPGK010000454         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_582468, whole genome shotgun sequence.
ACCESSION   DASPGK010000454 DASPGK010000000
VERSION     DASPGK010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582468"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="pilM"
                     /locus_tag="VEC56_12750"
     CDS             <1..663
                     /gene="pilM"
                     /locus_tag="VEC56_12750"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXV15068.1"
                     /translation="NQMEILLVAAKKEMVNAHAGIVKDAGLNPVIIDVDSFAIQNAVE
                     ATGENGAGRVVGLVNIGSDVTNINIIQNNIPYFTRDLSVGSNVFVEAVQRDLGVSFDE
                     AESLLSGSSEIDDANRFRQIIADAATDISMGIERSIAFLKTAGDAERIDHAVLSGGGA
                     CVPFLREILSEKHGIDFAINRAVSQVARAEGVFESAGDDVEKVGPILTVALGLALRRE
                     AK"
     gene            664..1227
                     /locus_tag="VEC56_12755"
     CDS             664..1227
                     /locus_tag="VEC56_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXV15069.1"
                     /translation="MIRINLLPSDDRKAKRKISLPTISGGAVLWVSLGLLVYAGAVGG
                     IWVLQNKKINDYETKILALKEESARLAPQLAKIRKLQAEREEVNKRLAIIAALDRDRY
                     LHVRMMNDLAVNVPDNCWLTDVAESGGSKVRIEGITFSNFIIADLMTSLEGSGRFGEV
                     ALARAEEGDIEGVRVVKFSVSASLMAN"
     gene            1257..1931
                     /gene="pilO"
                     /locus_tag="VEC56_12760"
     CDS             1257..1931
                     /gene="pilO"
                     /locus_tag="VEC56_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXV15070.1"
                     /translation="MNLKEPKTQRIVFGVIGLAAAGYVYFGTTFLPFNYPVRKAKIEA
                     LEKEHEKLAAEVEKARRVVGDLARLEAEYERLHDQWLSAQELLPEEKEMPELLRQVTM
                     AGSRAGIEFALFQPGAQVGKEFHTEHPITVTVRGNYHQVGSFLGRLSNMERIVNVSTL
                     ALGQPKEAKGKKKTSTKRKKADEAAPATALRSNNTVEAKFTLTAYTLMGGVTSETVDD
                     QHASND"
     gene            1924..2412
                     /locus_tag="VEC56_12765"
     CDS             1924..2412
                     /locus_tag="VEC56_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15071.1"
                     /translation="MTRLAVCLAVLAAVAVAQAQEPAKNAPATAKDAPAPATTKADST
                     APATPAVTQSGKVVLRENLYYYDAFNRRDPFRSLIDGAFNQSDRMDRVNLSAVQLVGI
                     VRGEVDRFALLEDASGYSYILRVGDRVHNGSVVSIGEDELVARVTNFGQTTTVRLRLV
                     GR"
     gene            2424..>4083
                     /locus_tag="VEC56_12770"
     CDS             2424..>4083
                     /locus_tag="VEC56_12770"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXV15072.1"
                     /translation="MMNRLSGTQLTIIATLFAGVVLLASVAGGDGTNLDSIELAQTPT
                     ATTYMINPSGVVQYQEYALEDPKRLVIDLVGVKNVVESTKLQTDGRFVSAVRASQFQS
                     EPDLVTRVVFELAEGTKYKVSRKGTAVEVVFSGPAAGVMGATMGAAAPVAPTTDASPE
                     ENAWFEVPDEPAADAAPAPTEATKPTDAKPADAAKPADAKPAEAAKPADSKPADAAKP
                     ADAKPAETAKPSDAPAPAPKNAPAPAAQSATSAPAPAAQSAPAPAPKSAPAPANAALI
                     SDMPTRSPAYTDDASSEWGDIEAAWPQPSEFAAQASPSDEVVTAQNAPSRVPAEMSTP
                     LYGSALANQPMTIDVQGADIKTVLRSISEFSGVNIVAAPDVEGPVVIHLKNVPWKEAL
                     DTILRSQGYGWRDDYGIIRVAPIEQLQQDEVQAVTVDRKKEENASLQTRVVKLNYLNA
                     KEIKTAMNKVVSPRGSVEYEPGTNSVVIAEVPARIDRIVQMIIDMDTKLRQVEIVAKM
                     VDVDHKYTRELGVSWQALNLSTGQLAGDVVAGQRVLSPFTTVRVG"
BASE COUNT          805 a         1314 c         1351 g          613 t
ORIGIN      
        1 aaccagatgg agattctcct ggtcgccgcc aagaaagaga tggtcaacgc gcacgccggt
       61 atcgtcaagg acgccggctt gaacccggtc atcatcgacg tcgattcgtt cgcgatccaa
      121 aacgcggtcg aggcgacggg cgagaacggc gcgggtcgcg tggtggggct cgtgaatatc
      181 ggctccgacg tgaccaacat caacatcatc cagaacaaca tcccgtactt cacgcgcgac
      241 ttgagtgtcg gtagcaacgt cttcgtggaa gccgtgcagc gcgatttggg cgtgtcgttc
      301 gacgaggccg agagcctgct ctccggctcc tcggagatcg acgacgccaa ccgcttccgg
      361 cagatcatcg cggacgcggc caccgacatc tcgatgggta tcgagcgctc gatcgcgttc
      421 ttgaagaccg cgggcgacgc cgagcgcatc gaccacgcgg tcctctcggg cggcggcgcg
      481 tgcgtgccgt tcctgcgcga gatcctctcc gagaagcacg ggatcgattt cgcgatcaat
      541 cgcgcggtgt cacaggtcgc gcgcgccgaa ggcgtgttcg agtcggcggg cgacgacgtc
      601 gagaaggtcg ggccgattct caccgtggcc ctcggcctcg cgttgagaag ggaggcgaag
      661 tagatgattc ggatcaacct gcttccgtcc gacgaccgca aggccaagcg gaagatcagc
      721 ctcccgacca tctcgggcgg agcggtgctg tgggtgtcgc tgggtctgct cgtgtacgcg
      781 ggcgcggtgg gcggcatctg ggtgctgcag aacaagaaga tcaacgacta cgagaccaag
      841 atcctggccc tcaaggaaga gtcggcgcgc ttggcgccgc agctggccaa gatccgcaag
      901 ctccaggcgg agcgcgaaga ggtcaacaag cgtctcgcca tcatcgcggc gctcgaccgg
      961 gatcgctacc tgcacgtgcg catgatgaac gacctcgcgg tcaacgtgcc ggacaactgc
     1021 tggctcacgg acgtggccga gagcggcggg tccaaggtga ggatcgaagg catcacgttc
     1081 tcgaatttca tcatcgccga cttgatgacg agcctcgagg gctcgggtcg gttcggagaa
     1141 gtggccctcg cgagggccga ggaaggcgac atcgaaggcg tgcgcgtcgt gaagttctcg
     1201 gtcagcgcgt cgctgatggc taactaatcg cggctctgga atagggaagg gcaaccatga
     1261 acctcaagga acccaagaca caacgaatcg tcttcggcgt gatcggattg gccgcggccg
     1321 ggtacgtgta cttcggtacg acgttcctgc cgttcaatta tccggttcgc aaggcgaaaa
     1381 tcgaggcgct cgagaaagag cacgagaagc tcgccgccga agtcgagaag gcgcgccgcg
     1441 tggtcggcga cctggcgcga ctcgaggcgg agtacgaacg cctgcacgac cagtggctct
     1501 cggcgcagga actcctgccc gaagagaagg aaatgccgga gctcttgcgc caggtgacca
     1561 tggccggcag ccgcgcgggc atcgagttcg cgctcttcca gcccggcgcc caggtgggca
     1621 aggagttcca caccgaacac ccgatcacgg tgacggtgcg tggcaattac caccaagtgg
     1681 gatcgttctt gggtcgcttg tcgaatatgg agcgtatcgt gaatgtctcc acgctcgcgc
     1741 tcgggcagcc caaggaagca aagggcaaga agaagacgag caccaagcgg aagaaggccg
     1801 acgaagcggc acccgcgacg gccttgcggt ccaacaacac cgtcgaggcc aagttcaccc
     1861 tgactgccta cacgttgatg ggaggtgtca ccagtgagac cgtcgacgat cagcatgcca
     1921 gcaatgacta ggctcgcggt gtgtctcgcg gtattggccg cggtggcggt ggcgcaggcg
     1981 caagagccgg ccaagaacgc cccggcgacg gccaaggacg cccccgcgcc cgctacgacc
     2041 aaggccgact cgaccgcgcc cgccacgccg gcggtgacgc agtccggcaa agtcgtgctg
     2101 cgcgagaatc tctactacta cgacgcgttc aatcgtcgcg atcccttccg gtcgctgatc
     2161 gacggcgcgt tcaaccagag tgaccgcatg gaccgcgtca atttgagcgc ggtacagctg
     2221 gtcggcatcg tgcgcggaga ggtcgatcga ttcgccctcc tcgaggacgc cagcggatac
     2281 agctatatcc tccgcgtcgg cgaccgcgtg cacaacggaa gcgtggtttc aatcggtgaa
     2341 gacgagttgg tggctcgcgt caccaacttc ggccagacga cgacagtcag actgcgcctc
     2401 gtcggacgtt aagaaggagt ggaatgatga atagactgtc tggtacgcaa ctcacaatca
     2461 tcgcgaccct cttcgcgggc gtggtcttgc tcgcgtcggt cgcaggaggc gatgggacga
     2521 acctcgattc gatcgagctg gcccagacgc cgaccgcgac gacgtacatg atcaacccct
     2581 cgggcgtggt ccaatatcag gaatacgcgc tggaagatcc caagcgtctg gtcatcgatc
     2641 tggtcggcgt gaagaacgtc gtcgagtcga ccaagctcca gaccgacggg cgcttcgtca
     2701 gcgccgtgcg cgcgagccaa ttccagtcgg agcccgactt ggtgacgcgc gtcgtgttcg
     2761 agctggccga aggcacgaag tacaaggtgt cccgcaaggg cacggccgtc gaggtcgtgt
     2821 tctcgggtcc ggccgccggc gtgatgggcg ccaccatggg tgcggcggcg ccggtcgcac
     2881 ccacgacgga cgcttctccg gaggagaacg cgtggttcga ggtaccggac gagccggccg
     2941 ccgacgccgc gcccgctccg acggaggcga ccaagcccac ggatgccaag cccgccgatg
     3001 cggccaagcc cgccgacgcg aagcctgccg aggcggccaa gccggccgac agcaagcccg
     3061 ccgacgcggc caagcccgcg gacgcgaagc ctgccgagac ggccaagccg agcgatgccc
     3121 cggcgcccgc gccgaagaac gctcccgcgc ccgccgcgca gagtgcgacc agcgctcccg
     3181 ccccggccgc gcagagcgct ccggcgcccg cgccgaagag cgctccggcg cccgcaaacg
     3241 cggccctcat tagcgacatg ccgacgcgct cgcccgcgta caccgacgat gcatcgtcgg
     3301 agtggggcga catcgaagcc gcctggccgc agccgtccga gttcgcggcc caggcgagcc
     3361 cgagcgacga agtcgtcacg gcccagaatg cgccctcgcg cgtgcccgcg gagatgtcca
     3421 ctcctctcta cggcagcgcg ctggccaacc agcccatgac gatcgacgtc caaggcgccg
     3481 acatcaagac ggtgctgcgc agcatctccg agttctcggg tgtcaacatc gtcgcggccc
     3541 ccgacgtcga gggcccggtc gtgattcacc tgaagaacgt cccttggaag gaagcgctcg
     3601 acaccatcct gcgtagccag ggctacggat ggcgcgacga ctacggcatc attcgtgtcg
     3661 ctcccatcga gcagctccag caggacgaag tccaggccgt cacggtcgat cgcaagaagg
     3721 aagagaacgc ctcgctgcaa acgcgcgtgg tgaagctcaa ctacctcaat gcgaaggaaa
     3781 tcaagacggc gatgaacaag gtcgtgtcac cgcgcggttc ggtcgagtac gagccgggca
     3841 ccaactcggt ggtcatcgcc gaagtgcctg cgcgcatcga ccgcatcgtg cagatgatca
     3901 tcgacatgga caccaagctc cgccaggtgg agatcgtggc caagatggtc gacgtcgacc
     3961 acaagtacac gcgcgagctg ggtgtcagct ggcaagcgct caacctctcg accggacagc
     4021 tggcgggcga cgtcgtcgcc ggtcagcgcg tcctgtcgcc gttcacgacc gtccgggtcg
     4081 gga
//
LOCUS       DASPGK010000455         6977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_817153, whole genome shotgun sequence.
ACCESSION   DASPGK010000455 DASPGK010000000
VERSION     DASPGK010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817153"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEC56_12775"
     CDS             <1..226
                     /locus_tag="VEC56_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15073.1"
                     /translation="GRVKIVQRRTDRVEVWTSSAVPVFHLARCIVERERESKTIPAVR
                     GIPESGPRRSRTTSVVVAFGKGAKSLVSSP"
     gene            266..2650
                     /locus_tag="VEC56_12780"
     CDS             266..2650
                     /locus_tag="VEC56_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXV15074.1"
                     /translation="MTRSSLRVHWVVVAGWCLAASAALVAPALADEIQPIDIRRPVSL
                     LGAERTYWIGSGATPEDAMALREALVAAGARNVNLFVPDMVVVADVPPAVAVAVSRVA
                     TPAAFYAQRARDLRATAVADLSWGWIVEGYALADRVSRGDGTGALASDPNDPESFRDV
                     VMTLSPARVDEIQRQVEANQRALDVTGTRPAVARRVNQNSEFLSGYILANFIYPESNG
                     VRDPNTENWTDDDLRQAKAGSTGAFVSWQGKFPNMDIAFVINHYERIPTDYEPISHDM
                     NTDGRWITDTMREMGWGLFLDDAQPIVHEFNEAERARWRTQWVVTSFIATSRNTPGYR
                     FKNGSANYTAYAFLGGPYMVEPFPAGSDPNLVGETLVYSQIVNHEIGHLFYTLDEYPG
                     APGVCTSRSGYLDIDNRNVTMTDPGGGQARCIPHVPCIMHTAARLDQNRPWCDWSQGH
                     LGVLDGNGNAIPDIYEAEPEITFVPEGPETVTTNSYTLRFKAKATAVPNRNPMQGEGR
                     VSYTLPLDEGKLIFGALQIRLDPVDGRWDEIQEEAEFSMTIPQAGQHVVLVVQVENRA
                     GFRSTVATKTVYFAGVRYSRLIAIPKRDVVIVSWETIGETFGARFDLYRIEQGQSMPG
                     TLIAQNVRPYETGHSGQPMYHVYDTDVRPGADYRYYVEGVFDLPYEGGTREYRSQSKI
                     VAQTAMLGLVDIVSDVAPNPTRGSVTFSVAVPASFEDTSFGQAPIPTAVDIRVYNVRG
                     QLVRTLKTTSELNSVITMRWDGTYQDGTAAPSGIYFLRVKSGEEEAVRKIVMLR"
     gene            2830..3828
                     /locus_tag="VEC56_12785"
     CDS             2830..3828
                     /locus_tag="VEC56_12785"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV15075.1"
                     /translation="MSDRLRHVNYAETVLDTIGNTPLIKLNRVTEGVACTVLAKLEQF
                     NPGGSVKDRIGIHMIRAEAEKGTLKPGGTIVECTSGNTGLGLALAASILGYQCVFTMP
                     DKVPLEKEYMLKAYGAEVIRCPTNVAPESPESYYSVAKRLVKERPNAILGNQYQNPMN
                     PDAHYRSTGPEIWKDTEGEIDYFVAGVGTGGTISGTGRYLKEQNPKVKVIGVDPVGSL
                     LTHYFHTRELIEAHQYLVDGIGEDFVPSTCQFQYVDDMIQVGDRESLIMTRRLAREEG
                     LLVGSSSGAVLVGALQVARDLDADKIMVVLIPDTGERYLSKVHSEPWLAEHGLSGK"
     gene            3850..4983
                     /locus_tag="VEC56_12790"
     CDS             3850..4983
                     /locus_tag="VEC56_12790"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HXV15076.1"
                     /translation="MGFYTDCVHAGQSSDEKTGAVITPIFQTSTYQQDGIGKHRGYEY
                     GRTQNPTREALERNVAALERAKHGLAYSSGMAATDTILRLFGPGDEIVVTHNVYGGTY
                     RLFAMVLQQYGLVFRFVDSADLREVERALTPRTKLVYLESPTNPMLRVTDIRAVADMA
                     HAIGARVCVDNTFMSPYLQTPIELGADLVVHSTTKFLNGHSDMVGGIVVLNDDAMAQR
                     LRFLQNAVGAVPGPMDCFLALRGTKTLAVRMRQHEENARVLARFLAGHKGLASVNYPG
                     LPDHPHHELAKKQARGFGAIISFDVGSLERADRVLRSVRLFTLAESLGGVESLISHPA
                     SMTHGSVPEGDRAKLGVTPGLVRISVGIEDVADLQADLDSALR"
     gene            5277..5456
                     /locus_tag="VEC56_12795"
     CDS             5277..5456
                     /locus_tag="VEC56_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15077.1"
                     /translation="MEKRDKNGVLMKDKEYVKYVQLLRKAERDGRLDGYPCPLCGSRY
                     VTKHDAEDCCRVIIS"
     gene            5471..5878
                     /locus_tag="VEC56_12800"
     CDS             5471..5878
                     /locus_tag="VEC56_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15078.1"
                     /translation="MIMPSETTSTIRQHCSVCKADFDMDVIKQGSTEDVLWLKCPGCQ
                     GFLPYMPKEDLTDVEDRVPDEQKFALEDLDVEHAKEYAEDQEYQIGEVLHHRSWNDFG
                     KVVAKDKLPGNRRTIWVQFIRQGRVQLLEGVVH"
     gene            5896..6675
                     /locus_tag="VEC56_12805"
     CDS             5896..6675
                     /locus_tag="VEC56_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15079.1"
                     /translation="MMHHSRWTAFACAIATAVLSIRADAAGVYENAATESALLVSPQR
                     TVVRGTLEIGAGGGVDATLFRVLGAFPARSWFLFAVEMPFVSVSAPDGIESGPGDLFL
                     RARARVWGSNGRALSLLGTVGTGTGNRRFFPYSSQTLDVNASVGFVDSVGAMQPFVVV
                     GYEWMNRFDEWRYTPDTSPANHLRATAGTDLDLGENSDLRGGVMFHWYDGGAERTLVF
                     AGLGYQWTPTFRLLAEGQVEAGPQAQRVGNWSLTAGLSVLF"
BASE COUNT         1252 a         2278 c         2275 g         1172 t
ORIGIN      
        1 gggacgggta aagatcgttc aacggcgcac cgaccgcgtc gaagtgtgga cgtcgagtgc
       61 ggtgccggta ttccacctcg cgcgttgtat cgtcgagcgc gagcgggaat cgaagacgat
      121 cccggccgtg cgcggcatcc cggaatcggg accgcggcgc tctcggacga ccagcgtcgt
      181 cgtcgccttc ggcaaaggcg cgaagtcgct cgtctcgtcc ccctgagtct tggagacgag
      241 gtctttgaac ctgggagatt cgactatgac gcgctcttct ctgcgagtgc attgggtcgt
      301 ggtggcggga tggtgtctgg cggcatccgc cgcgttggtg gcaccggcct tggccgacga
      361 aatccagccg atcgatattc gaaggccggt gtcgctcctg ggcgccgagc gcacctactg
      421 gatcggctcg ggtgccacgc ccgaagacgc catggccctg cgcgaagcgc tggtagccgc
      481 gggcgcccgt aacgtcaatc tcttcgtgcc cgacatggtc gtcgtcgccg acgtgccgcc
      541 cgccgtcgcg gttgcggttt cgcgcgtcgc cacgccggcc gcgttctacg cccagcgcgc
      601 gcgcgacctg cgcgcgaccg ccgtcgccga cctctcctgg gggtggatcg tcgaaggcta
      661 cgcgctcgcc gaccgcgtct cgcgcggtga cggtaccggc gcgctcgcgt ccgaccccaa
      721 cgatcccgag tcctttcgcg acgtcgtgat gacgctctcg cccgcgcgcg tagacgagat
      781 ccaacgccaa gtggaggcca accagcgcgc gctcgacgtt accggcaccc gccccgcggt
      841 ggcgcggcgc gtcaaccaga actccgagtt cctctccggc tacatcctcg cgaatttcat
      901 ctaccccgag agcaacgggg tgcgcgaccc caacaccgag aactggaccg acgacgatct
      961 gcgccaggcc aaggccggct ccaccggcgc gttcgtttcg tggcagggca agttccccaa
     1021 catggacatc gccttcgtca tcaatcacta cgagagaatc ccgaccgact acgaacccat
     1081 ctcgcacgac atgaacaccg acgggcgctg gattactgac accatgcgcg agatggggtg
     1141 ggggctcttc ctggacgacg cgcagcccat cgtccacgag ttcaacgagg ccgagcgcgc
     1201 gaggtggcgc acgcaatggg tggtcacgtc gttcatcgcg accagccgca acacgcccgg
     1261 ctatcgcttc aagaacggga gcgcgaatta caccgcctac gcgttcctcg gcggccccta
     1321 catggtggag ccgtttcccg ccggcagcga tcccaacctg gtgggcgaga cgctcgtgta
     1381 ctcgcagatc gtgaaccacg agatcggcca cttgttctac acgctcgacg aataccccgg
     1441 tgcgcccggt gtttgcacgt cgcgctcggg ttatctcgac atcgacaatc gcaacgtgac
     1501 catgaccgat cccggcggcg gccaggcgcg ctgcataccg cacgtgccct gcatcatgca
     1561 cacggcggcg cggctcgatc agaatcggcc gtggtgcgac tggagccagg gacacctggg
     1621 cgtcttggac ggcaacggca acgcgatccc cgacatctac gaggccgagc ccgagatcac
     1681 cttcgttccc gagggaccgg agacggtgac gaccaacagc tacacgctgc gcttcaaggc
     1741 caaggcgacc gcggtcccca accggaaccc gatgcagggc gaggggcgcg tgagctacac
     1801 gctcccgctc gacgagggca agctgatctt cggcgcgctc cagatccgcc tcgatccggt
     1861 cgacgggcgc tgggacgaga tccaggaaga ggcggagttc tcgatgacga tcccgcaggc
     1921 ggggcagcac gtggtgctgg tggtccaggt cgagaaccgg gccggcttcc gttcgaccgt
     1981 cgcgactaag acggtgtatt tcgcgggcgt gcgctacagt cgcctcatcg cgatacccaa
     2041 gcgcgacgtc gtgatcgtgt cgtgggaaac gatcggggag acgttcgggg cgcgcttcga
     2101 cctgtaccgg atcgagcaag gccagtcgat gccggggacg ctgattgcgc agaacgtccg
     2161 tccatacgaa accgggcata gcggccagcc catgtaccat gtctacgaca ccgacgtgcg
     2221 acccggagcg gactatcggt actacgtcga gggcgtgttc gatttgccgt acgaaggcgg
     2281 tacgcgcgag taccgctcgc agtccaagat cgtggcgcag accgcgatgc tgggactggt
     2341 cgatatcgtc tccgacgtgg cgcccaaccc gacgcgcggc agcgtcacct tctcggtcgc
     2401 cgtgcccgcg tcgttcgagg atacgtcgtt cggccaggca cccattccga ccgcggtcga
     2461 cattcgcgtg tacaacgtgc gcgggcagtt ggtgcgtacg ctcaagacca cgagcgagct
     2521 caattcggtg atcacgatgc gatgggacgg cacgtaccaa gacggcacgg cggcaccctc
     2581 cggcatttac ttcctgcgcg tcaagtcggg cgaagaagag gccgtccgca agatcgtgat
     2641 gctccgctga cgcgcctggc cggcgtgcgt cgtgcagccg cggcgcgttt gcgtcggcac
     2701 gaccgatgtg atataccagg gcgagaagcc agcgggctcg ccgccgcaat cgcgagcccc
     2761 gacgcccatc ccgcatacta cccaacgacc caaggaggca ggcgcacgcc cgtgtgccgc
     2821 aaggaccgca tgagcgatcg actgcgccac gtcaactacg cggaaaccgt cctcgatacc
     2881 atcggcaaca cgccgctgat caaattgaat cgcgtgaccg aaggcgtggc gtgcaccgtg
     2941 ctcgccaagc tcgagcagtt caaccccggc gggagcgtca aagaccgcat cggcatccat
     3001 atgatccggg ccgaggccga gaaggggacg ctcaagccgg gcggcaccat cgtcgagtgc
     3061 acgtcgggta acaccggact cgggttggca ctcgccgctt cgatcctggg ataccaatgc
     3121 gtgttcacga tgccggacaa agtcccgctc gagaaggagt acatgctcaa ggcgtacggc
     3181 gcggaagtga ttcgctgccc gaccaacgtg gcgccggaat cgcccgagag ctactactcg
     3241 gtggcgaagc gcctggtgaa ggagcggccc aacgcgatcc ttggcaacca gtaccagaac
     3301 ccgatgaatc cggatgcgca ctaccgctcg accggacccg agatctggaa ggacaccgaa
     3361 ggcgagatcg actatttcgt ggcaggcgtg gggacgggcg gaaccatcag cggcaccggg
     3421 cgctatctga aagagcagaa ccccaaggtc aaggtaatcg gcgtcgatcc ggtgggcagc
     3481 ctgcttacgc actatttcca taccagggaa ctgatcgagg cgcaccagta cctggtcgat
     3541 ggcatcggcg aggatttcgt tcccagcacg tgccagttcc agtacgtcga cgatatgatc
     3601 caggtgggcg accgcgagtc gctgatcatg acgcggcggc tcgcgcgcga agaggggctg
     3661 ctggtgggca gctcgtcggg ggcggtgctg gtgggggcgc tgcaagtggc gcgcgacctc
     3721 gacgccgaca agatcatggt ggtgctcatc cccgacacgg gcgagcgcta cttgagcaag
     3781 gtgcactcgg aaccgtggct cgccgagcac gggctctcgg gcaagtagca ccgtttcgat
     3841 aggagaaaca tgggattcta cacggattgc gtccacgccg ggcaatcgtc cgacgagaag
     3901 accggcgctg tcatcacgcc gatcttccag acctcgacct accagcagga cggcatcgga
     3961 aagcaccgcg gttacgagta cgggcgcacc cagaatccca cccgcgaagc gctggagcgc
     4021 aacgtggcgg cactggagcg cgcgaagcac ggactggcgt attcgtccgg catggccgcg
     4081 accgacacca tcctgcgctt gttcggcccc ggcgacgaga tcgtcgtcac gcacaacgtc
     4141 tacggcggca cctatcggct gttcgcgatg gtcctgcagc agtacggcct ggtgttccgc
     4201 ttcgtcgact cggccgacct gcgcgaagtc gagcgcgccc tgacccccag gaccaagctg
     4261 gtctatctcg aatcgcccac caacccgatg ctacgcgtca ccgacatccg cgcggtcgcg
     4321 gacatggcgc acgccatcgg cgcgcgggtg tgcgtcgaca acaccttcat gagcccctac
     4381 ctgcagaccc cgatcgaatt gggcgcggat ctggtggtgc acagcacgac caagttcttg
     4441 aacggccaca gcgacatggt gggcggcatc gtggtcctga acgacgacgc catggcgcag
     4501 cggcttcgct tcctgcagaa cgccgtgggc gcggtgccgg ggccgatgga ttgcttcctc
     4561 gccttgcgcg gcaccaagac cctcgcggtg cgcatgcgcc agcacgaaga gaacgcgcgc
     4621 gtcctggcgc gcttcctcgc gggccacaag ggtctcgcga gtgtcaacta cccgggcctc
     4681 cccgatcacc cgcaccacga gctcgcgaag aagcaggcgc gcggttttgg cgccatcatc
     4741 tcgttcgacg tgggctcgct cgagcgcgcc gatcgcgtgc tgcgatccgt gagattgttc
     4801 acgctcgcgg agagcttggg cggggtcgag agtttgatca gccaccccgc gtcgatgacg
     4861 cacggcagcg ttcccgaagg ggatcgcgcc aagctcggcg tgacaccggg gctggtgcgc
     4921 atctcggtcg gcatcgaaga cgtcgccgat ttgcaagccg acctcgattc cgcgctgcgc
     4981 tagcggagcc gcgcgccgcg ccaccacgcc gcgtaggaaa attcgccgcc ttgcgcgatg
     5041 cgcgatgcgt ggatatattc catgcgtcga tgttgccgca cgacgactct gttagttgtc
     5101 catgagtgct gattcttccg cagtttttgc ttgacggcct cttgtggctt cccctagggt
     5161 tgccgcaaga tattgtaccc tgggcacgtc aggacacagc atcctgtgta tcccggccat
     5221 tattaacggt ttaggcggat gcctggtttt ttcacctcaa cgcggaggag ttgggcatgg
     5281 aaaagcggga caagaacggg gtgctcatga aggacaagga gtacgtgaag tacgtacagc
     5341 tcctgcggaa ggcagagcgt gatgggcggt tggatgggta tccgtgtccg ttgtgcggat
     5401 cgcggtacgt gacgaagcac gatgcggagg actgctgccg cgtcatcatc tcttgatgga
     5461 cggtcgcacg gtgataatgc ccagcgagac gacgagtacg attcgacagc attgctcggt
     5521 ctgcaaggcc gacttcgaca tggacgtcat taagcagggg agcacggaag acgtgctctg
     5581 gctcaagtgt ccgggctgcc agggattcct tccctacatg cccaaggaag acttgaccga
     5641 tgtcgaagat cgggtgccgg acgagcagaa gtttgcgctg gaggatctcg acgtcgagca
     5701 cgcgaaggaa tacgccgaag accaggagta ccagatcggt gaggtgttgc accaccggtc
     5761 ctggaacgac ttcggcaaag tcgtcgcgaa agacaagctc ccgggcaatc gccgcacgat
     5821 ctgggtccag ttcatccgac aggggcgtgt ccaactcctc gagggcgtcg ttcactagcg
     5881 gcgcctccgg ggttcgtgat gcaccatagc cgttggacgg cgttcgcgtg cgcgatcgcc
     5941 accgcggtat tatcgattcg ggccgacgcc gccggcgttt acgaaaacgc cgccacggag
     6001 tcggcccttc tcgtttcccc ccagagaacg gtcgtgcgcg gcaccctcga gatcggggcc
     6061 ggcggcggcg tggacgcgac cctgttccgc gtcctgggcg cgtttcccgc gcgctcgtgg
     6121 ttcctgtttg cggtcgagat gccgttcgtg tcggtgagcg cgcccgacgg catcgaatcg
     6181 ggcccgggcg acctgttcct gcgcgcccgc gcgcgggtgt ggggttcgaa cgggcgcgcg
     6241 ctttcgctcc tgggtaccgt cgggaccggc accgggaatc gccgcttctt tccctattcc
     6301 tcgcaaacgc tggatgtcaa cgcgtcggtc ggattcgtcg actcggtcgg ggccatgcag
     6361 cccttcgtcg tcgtgggcta cgagtggatg aaccgcttcg acgagtggcg ctacaccccc
     6421 gacaccagcc ccgccaacca cctgcgcgcg accgccggca ccgacctcga tctgggcgag
     6481 aacagcgacc tgcgcggcgg cgtcatgttc cactggtacg acggcggcgc ggaacgcacc
     6541 ctcgtcttcg cgggcctggg ctaccaatgg accccgacgt ttcgcctgct ggccgaggga
     6601 caagtcgaag ccgggccgca agcgcagcgc gtcgggaact ggtcgctgac ggcggggctg
     6661 tcggtattgt tctaatcgat cctgaggacc cgcccgctcc cacgcgaaag cgacgcaccc
     6721 gttcgcagaa tcgtccacgg ggcgccgcgg tgttcccgaa aacgcctcgc gcgatcgttc
     6781 cgattgcggc gcgcgagggg gcgactattc ccgcttcttc cgcatacgga cgcctcaaaa
     6841 tccacatcgt gcgtggcaca tcgattgcca atctaccccc gaccgcggcc actccaggtc
     6901 gcgccaccat ctcggtccaa gacggccggt tgggttacag ggtggcgctg gactccaagg
     6961 aactcctggc ctggtcg
//
LOCUS       DASPGK010000456         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_350046, whole genome shotgun sequence.
ACCESSION   DASPGK010000456 DASPGK010000000
VERSION     DASPGK010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350046"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VEC56_12810"
     CDS             <1..146
                     /locus_tag="VEC56_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXV15080.1"
                     /translation="ARGVAAEMGVAESGYRLVMNTHAGAGQSVFHIHLHVLGGRGFAW
                     PPG"
     gene            complement(151..522)
                     /locus_tag="VEC56_12815"
     CDS             complement(151..522)
                     /locus_tag="VEC56_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HXV15081.1"
                     /translation="MSSEFWEAVERIRAGDARYARDAYAFVMDSLDYTMRAVGERRHV
                     SAAELVRGLCDHARQRFGLFAYTVFAKWGLASSDDVGEIVFQLIDAGILSRQESDSRA
                     DFDGVVDFRDALEASSLEGEG"
     gene            complement(519..1655)
                     /gene="bshA"
                     /locus_tag="VEC56_12820"
     CDS             complement(519..1655)
                     /gene="bshA"
                     /locus_tag="VEC56_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HXV15082.1"
                     /translation="MPPLKVGIVCYPTLGGSGVVATELGKSLAKLGNEVHFITTGHPS
                     RLSGYDERVYLHKVVTGEYPLFQQYAPYSLSLAVKIREVAEQYRLDLVHVHYAIPHAA
                     SAFLAKEMLKPRRLPTMTTLHGTDITLVGMMPSYYEITRFSIEKSDAVTAVSEFLRRE
                     TVREFHIEHPIEVIHNFVDTELFRLEEQPCAARARLSPRGERVLMHISNFRKVKNLPI
                     VLQVFDEIRKHVPARLALVGDGPEREATERQAEEMGIADRVDFLGDQESVSDILPAAD
                     VFLLPSQHESFGLAALEAMACGVPVVGSRIGGLPEVIVHEETGYLCDPTDVPCMTAIV
                     MGLLRNEELRRSIGRAARARAEREFNRDLIVGRYVDAYRRLVGS"
     gene            complement(1661..2383)
                     /gene="bshB1"
                     /locus_tag="VEC56_12825"
     CDS             complement(1661..2383)
                     /gene="bshB1"
                     /locus_tag="VEC56_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HXV15083.1"
                     /translation="MRVLAIGIHPDDVEIGCGGTVALLARRGDEVAVLDLTRGESSTN
                     GTPEERAREAAEAARTLGVARRFNAALPDTGLRSEEPDQVRAIVRFIRAERPHVLLIP
                     NADDPHPDHAAGGLLSRHALFLANVNGYLTEEGKRRQERWRVPRALLYGGRKEVRADL
                     VVDITPVHEAKLAAIRAHASQVGGGEGTLATPLTDPRFLSVIEARDRIAGRKVGATFG
                     EAFELLAPLAVTDFTALVPPGT"
     gene            complement(2385..3980)
                     /gene="bshC"
                     /locus_tag="VEC56_12830"
     CDS             complement(2385..3980)
                     /gene="bshC"
                     /locus_tag="VEC56_12830"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HXV15084.1"
                     /translation="MSALRAEVPIDRLPGTTTLFRDFVARADAPVHRRLRGFRAQDEA
                     WRHVLGTARGVDARLVERMAAENQALGASRETIERVRGLADGRTRAVITGQQPGVAGG
                     PLLSLYKAASAVALAREIEVRWKTPCVAVFWLGSDDDDFAEIRDLFLVSDTLSVVGVS
                     LDGTAHAPGRRVGDVAGTAVARSWDAVSPFLPKGDSAARIDAIVRGGDDLGRIAARVL
                     MELAHGSIAVIDGREPLLRDSARTTILEFFDREDAIRGLVVAGGDALLADGYHAQLDS
                     SSDSGLFLVREGTRQRIPAEARATARAAFAHDVGAASPGVVARNLIQDSVFAPVAVVL
                     GPAEIAYRAQLARVYDIMGVDTPVVFPRLGATFVPPAVAEAVERSGTDATLLATDPSE
                     WVVRVMRSLESPRAAEAARAFEGAFRADAARFIESASGRLDPRAREKLERRVADLANR
                     VASVAQGAVEQDALAGASQWPWLARASDLFARDGAPQERFLSALVPQTFHDHDAWGLV
                     LEVASDHVRDALDGTVLHRVYSR"
     gene            complement(3977..>4527)
                     /locus_tag="VEC56_12835"
     CDS             complement(3977..>4527)
                     /locus_tag="VEC56_12835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV15085.1"
                     /translation="LSPYLLSDAVASRALRGRVVLGDMRVLPFRDGAVGAVVNMFTSF
                     GYFETDLDNVRVMHEAARVMTPGGVFLMDFLNADALAQELRDGKPTERRERDAVVEER
                     REIVDDGRVLVKHVRVRVDAREPVEYHERLRLYRHHDLVAMAETSGLVARAVYGDYAL
                     TSYDARTSPRVIMVCAKPGGNA"
BASE COUNT          698 a         1643 c         1538 g          648 t
ORIGIN      
        1 cggcgcgcgg ggtcgcggcc gagatggggg tggcggaatc gggctatcgg ctggtcatga
       61 acacccacgc gggggctggg cagtcggtgt tccacatcca tctgcacgtg ctcggcggac
      121 ggggattcgc gtggccgccg ggttagcggg ctagccctcg ccttccagcg aggacgcctc
      181 cagcgcgtcg cgaaagtcga ccacgccgtc gaagtcggcg cgcgagtcgc tttcctggcg
      241 cgagaggatg ccggcgtcga tcaactggaa cacgatctcg ccgacgtcgt cgctggacgc
      301 gagcccccac ttggcgaaca ccgtgtaggc gaacaatccg aagcgctgcc tggcatggtc
      361 gcacagcccg cgcaccagct cggccgcgga tacgtggcgg cgctccccca ccgcgcgcat
      421 ggtgtagtcg agcgagtcca tgacgaacgc gtaggcatcg cgcgcgtagc gggcgtcgcc
      481 ggctctaatc cgctcgaccg cttcccagaa ctcggagctc acgaccccac cagccgacgg
      541 taagcgtcca cgtaacgacc gacgatcagg tcgcggttga actcgcgctc ggcccgggcc
      601 cgcgccgctc gtccgatcga ccggcgcagc tcctcgttgc gcaacagccc catcacgatc
      661 gcggtcatgc agggcacgtc ggtcgggtcg cacaggtaac cggtctcttc gtgcacgatc
      721 acttccggga gccctccgat gcgcgatccc accaccggca ccccgcacgc catcgcttcc
      781 agcgcggcca gcccgaagct ttcgtgctgg ctgggcagga ggaacacgtc cgcggccggg
      841 aggatgtccg acaccgactc ttgatcgccc aggaagtcga cccggtccgc gatccccatc
      901 tcctcggcct ggcgctcggt ggcctcgcgc tcgggcccgt cgcccaccag cgccagccgc
      961 gccggtacgt gcttgcggat ctcgtcgaac acctgcagca cgatggggag gttcttgacc
     1021 ttgcgaaagt tggaaatgtg catcagcacg cgctcgccgc gcggggacag gcgtgcgcgc
     1081 gccgcgcacg gttgctcctc gagacggaac agttccgtat cgacgaaatt gtggatcact
     1141 tcgatcgggt gctcgatgtg gaactcgcgc acggtctcgc ggcgcagaaa ctccgagacg
     1201 gccgtcaccg cgtcgctctt ctcgatgctg aagcgcgtga tctcgtagta cgacggcatc
     1261 atgcccacga gtgtgatgtc ggtcccgtgc agcgttgtca tggtgggcag gcgccggggc
     1321 ttgagcattt ccttggccag gaacgcggac gccgcgtgcg ggatggcgta atggacgtgc
     1381 accaggtcga gccggtactg ctcggccacc tcccgaatct tgacggcgag gctcaacgaa
     1441 tagggggcgt actgctggaa cagcgggtac tcgcccgtga cgaccttgtg caggtagacg
     1501 cgctcgtcgt agcccgacaa gcgcgaggga tgtccggtgg tgatgaagtg cacctcgttg
     1561 ccgagcttcg ccagcgactt gccgagctcg gtggcgacca ccccgcttcc ccccagcgtc
     1621 gggtagcaca cgatgccgac cttgagagga ggcaccggcg tcacgtcccg ggcgggacca
     1681 gcgccgtgaa gtcggtgact gccaggggag cgagcaattc gaaggcctcg ccgaaggtcg
     1741 ccccgacctt gcggcccgcg atacggtcgc gcgcctcgat cacggacagg aaacgcgggt
     1801 cggtgagcgg cgttgcgagc gtcccctcgc cgcccccgac ttggctcgcg tgggcgcgaa
     1861 tggcggccag cttggcctcg tggacggggg tgatgtcgac gacgaggtcc gcgcgcactt
     1921 ccttgcgccc gccgtacagg agtgcccgcg gcacgcgcca gcgctcctga cgccgcttcc
     1981 cctcctcggt caggtaaccg ttcacgttgg cgagaaagag cgcgtggcgg gacagcagcc
     2041 cgcccgccgc gtggtcgggg tgggggtcgt cggcgttggg gatcaagagt acgtgggggc
     2101 gctcggctcg aatgaaccgc acgatggcgc gcacctgatc gggctcctcg gagcgcagcc
     2161 cggtatccgg cagcgcggcg ttgaagcggc gcgccacccc cagggtacgc gcggcctcgg
     2221 ccgcttcgcg cgcgcgctcc tccggcgtcc cgttggtcga cgattccccg cgcgtcaagt
     2281 cgagcacggc gacctcgtcg ccgcgacgcg cgagcagcgc gacggtcccg ccgcagccga
     2341 tctcgacgtc gtcggggtgg atgccgatgg ccagtactcg catgctaccg cgaatataca
     2401 cgatggagaa ccgtgccgtc taacgcgtcg cgcacgtggt cgctggccac ctcgagcacg
     2461 agcccccacg cgtcgtggtc gtggaacgtc tgcggcacca gcgcggaaag gaagcgctct
     2521 tgcggcgcgc cgtcgcgcgc gaacaggtcg gacgcacgcg ccagccacgg ccactgcgat
     2581 gcgcccgcga gcgcgtcctg ctcgaccgcg ccctgcgcga cactcgccac gcggttggcg
     2641 agatccgcca cgcgccgctc gagcttctcc ctcgcgcgcg ggtccaatcg tcccgacgcg
     2701 gactcgatga agcgtgccgc gtcggcgcga aacgcgccct cgaaggcgcg cgccgcttcg
     2761 gcggcgcgcg gactctcgag cgagcgcatc acgcggacga cccattcgga cgggtcggtg
     2821 gccagcaggg tggcgtcggt cccgctccgc tccacggcct cggcgaccgc cggcggaacg
     2881 aaggtcgcac ccaggcgcgg aaacaccacg ggcgtgtcga cccccatgat gtcgtacacg
     2941 cgcgcgagct gggcgcggta cgcgatctcc gccggtccca gcaccaccgc caccggtgcg
     3001 aataccgagt cctggatcag attgcgcgcc accacgccgg gtgaagccgc cccgacgtcg
     3061 tgcgcgaacg ccgcgcgagc ggtcgcgcgc gcctcggcgg gaatccgttg ccgcgtgccc
     3121 tcgcgaacca agaacagccc cgagtccgaa ctggaatcga gctgggcgtg gtatccgtcg
     3181 gccaagagcg cgtcgccgcc agccacgacc aggccgcgga tcgcgtcctc gcggtcgaag
     3241 aactcgagga tcgtcgtgcg cgcggaatcc cgaagcagcg gctcgcggcc gtcgatcacc
     3301 gcgatgctac cgtgcgcgag ctccatcagg acgcgcgccg cgatgcgccc cagatcgtcg
     3361 ccgccgcgca cgatcgcgtc gatgcgggcc gcggagtcgc ccttgggcag gaaggggctc
     3421 accgcgtccc aggaacgcgc gaccgccgtg cccgccacgt cccccacgcg ccggccgggt
     3481 gcgtgtgccg tgccgtcgag actcacgccg accaccgaaa gcgtatcgct caccaggaac
     3541 aggtcgcgaa tctcggcgaa gtcgtcgtcg tcgctgccca gccagaacac cgccacgcac
     3601 ggagtcttcc agcgcacctc gatctcgcgg gccagggcca ccgcgctcgc ggccttgtac
     3661 agcgacaaga gcgggccgcc ggccacgccc ggctgctgac ccgtaatcac cgcgcgcgtg
     3721 cgcccgtcgg cgagcccgcg cacgcgctcg atcgtttcgc gcgatgcccc cagtgcctga
     3781 ttctccgccg ccatgcgctc gaccaggcgc gcgtccacgc cgcgcgcggt gcccaggacg
     3841 tggcgccacg cctcgtcctg cgcgcgaaac cctcgcaggc gccgatgaac gggcgcgtcg
     3901 gcgcgcgcga cgaagtcgcg aaacagggtc gtcgtgccgg gaaggcggtc gatcgggacc
     3961 tcggcgcgaa gggcgctcac gcgtttcccc ccggcttggc gcacaccatg atgacgcgcg
     4021 gcgacgtacg cgcatcgtac gacgtgaggg cgtagtcccc gtacacggct cgcgctacca
     4081 gcccggaggt ctccgccatc gccaccaggt cgtggtgtcg gtacaggcgc aggcgctcgt
     4141 ggtactcgac cggctcccgg gcgtcgaccc gcacgcgcac gtgcttgacc agtacgcggc
     4201 catcgtccac gatctcgcgc cgctcttcca cgaccgcgtc ccgctcgcgg cgttcggtcg
     4261 gctttccgtc gcgcagttcc tgcgccagcg cgtccgcgtt cagaaaatcc atcaggaaca
     4321 cgcctcccgg cgtcatcacg cgcgcggctt cgtgcatgac gcgcacgttg tcaaggtcgg
     4381 tctcgaagta gccgaagctc gtgaacatgt tgaccacggc tccgacggca ccatcccgaa
     4441 acggcagcac ccgcatgtcg cccagaacca cccggccgcg cagcgcgcgc gacgccaccg
     4501 cgtcggacag aagatacggc gacaggt
//
LOCUS       DASPGK010000457         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1285371, whole genome shotgun sequence.
ACCESSION   DASPGK010000457 DASPGK010000000
VERSION     DASPGK010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285371"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VEC56_12840"
     CDS             <1..1521
                     /locus_tag="VEC56_12840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXV15086.1"
                     /translation="SLFKTLLVANRGEIALRVVRACREMGIRSVAVYSDADRLAPHVL
                     EADEAHRIGPPPSSESYLRIDSLLGAARKSGAEAVHPGYGFLSEREVFARAVEDAGLV
                     FVGPAPATLAAMGDKTEARRRMHAAGVPIVPGVVDALADPGAAARAAEEIGFPVLLKA
                     AAGGGGKGMRVVRSGSELGRAFEAASREALAAFGDGSVYLERFLDRPRHVEIQVLGDR
                     HGRVLHLGERECSIQRRHQKLVEEAPSPVLTPEQREEMGGSAVRAAAAVDYVGAGTVE
                     FLYDEARGAFYFLEMNTRIQVEHPITECTTGIDLVQWQLRIARGEALAFEQKDIGQRG
                     HAIECRIYAEDERKHFIPSCGVISLLREPAGPGVRVDSGVYQGWEVTSHYDPILAKLV
                     AFGETREVARRRMLRALDEYVVQGVATSVDLHKRILRHPSFVAAKVDTAFLDEHATDL
                     LEPAKADVPDLAFIAAALAAESGSGRGARATHAATNDDDAPSPWETTGSWEIGRAS"
     gene            1523..2023
                     /locus_tag="VEC56_12845"
     CDS             1523..2023
                     /locus_tag="VEC56_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXV15087.1"
                     /translation="MATITIDGVSRAVSVARSKDGFVVTVDGRRYDVREVARVAEGIA
                     FIVGNESHVARVTSGREGIELSIGAHSYVQARDLVDADRPAGAAGRHGDGRLEAPMPG
                     SIIGIHVSEGDRVRSGQPLVVLESMKMHNEIASPIDGVVRKINCRVGEQVAFGHVLVE
                     IGTEAS"
     gene            2020..2514
                     /locus_tag="VEC56_12850"
     CDS             2020..2514
                     /locus_tag="VEC56_12850"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXV15088.1"
                     /translation="MKSYSAPWGKLLIGLTAFSTLICVGVSVGVGLNVRSIPETEGVV
                     RAAAALPMLVVVIAALFMVRGYVVTADEIVVQRPLWSSRFERARLQSAAIDPDALRGS
                     IRLFGNGGLFSFTGWFRSPQLGRYRAYVTDPSRTVILRFADRIIVVSPSDPAAFVRDV
                     TPRG"
     gene            2534..3349
                     /gene="uppP"
                     /locus_tag="VEC56_12855"
     CDS             2534..3349
                     /gene="uppP"
                     /locus_tag="VEC56_12855"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HXV15089.1"
                     /translation="MPDIVLAAILGLVQAVTEFLPISSSAHLILARHVLDFEAVDGLT
                     FDVALHIGTLLAVVVYFWGDLRALARGFLGSVARRSGPADPAGRLAWYIIAACVPAGL
                     VGFFFEKAIEGYFRNPSVIVVTLLLGAFLFLWVERRFRHDGEMQALSLGQAVAIGAAQ
                     SLALIPGVSRSGITIVVGMMCGLRRDQAARFSFLMASPLMLAAGAKKALDLAGQSITS
                     AQLAVLAVGVATSAVAGWLVIRFLLDYLRRRGLDVFAYYRIVLAIVVAIVLLA"
     gene            3470..>4242
                     /locus_tag="VEC56_12860"
     CDS             3470..>4242
                     /locus_tag="VEC56_12860"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HXV15090.1"
                     /translation="MTGSSRRGLAFSLTASLAFTVSTLSVQASVKSHQKISDTAGNFI
                     APLNDDDRFGIGVTPLGDLDGDGVADIAVGAYLDDFYGDRFGAAYVLLLNADGTVKSH
                     VKIGMGLGGFTGDLDFWDEFGVSVTAIGDLDDDGVMDLAVGARLDDDSPSYVVDGVDR
                     GAVWILFLNANGTVKSHQKISDRFGGFTGGLSDGDWFGFYSTALGDIDGDGVEDIAVS
                     ATRDDDGGTDRGAVWILNLQANGMVKSQYKISGTQGGFTG"
BASE COUNT          616 a         1402 c         1486 g          738 t
ORIGIN      
        1 tctttgttca agacgctgtt ggtcgccaat cggggggaga tcgcgctccg tgtcgtgcgc
       61 gcctgccggg aaatgggcat ccggtcggtc gcggtctatt cggacgccga ccgcctcgcc
      121 ccgcacgtcc tcgaggccga cgaggcccac cggatcgggc cgcccccttc gtccgagagc
      181 tacctgcgca tcgatagcct gttgggcgcc gcgcgaaagt cgggcgcgga agcggtgcac
      241 ccgggatacg gcttcctctc cgagcgcgag gtcttcgcgc gcgccgtcga agacgccggg
      301 ctcgtcttcg tcggtcccgc tcccgcgacg ctcgccgcga tgggcgacaa gaccgaagcg
      361 cgccggcgca tgcacgccgc gggtgtgccg atcgtcccgg gcgtcgtcga cgcgctcgcc
      421 gacccgggag cggcggcccg agcggcggag gagatcggct ttccggtcct gctcaaggcg
      481 gcggcggggg gcggcggcaa ggggatgcgc gtggtgcgga gcgggtccga gctcgggcgc
      541 gccttcgagg ccgcgagccg cgaggcgttg gccgcgttcg gcgacggctc ggtctacctg
      601 gagcgcttcc tcgaccgccc caggcacgtc gagatccagg tgctcgggga ccggcacggc
      661 cgcgtgctgc acctgggaga gcgcgagtgc tcgatccagc gccggcacca gaagctcgtc
      721 gaagaagcgc cttcccccgt cctgaccccg gagcagcgcg aggagatggg gggctcggcg
      781 gtgcgggcgg ccgccgccgt ggactacgtg ggcgcgggga ccgtcgagtt cctctacgac
      841 gaggcgcggg gggcgttcta cttcctcgag atgaacacgc gcatccaggt ggagcacccg
      901 atcaccgagt gcacgaccgg gatcgacttg gtgcagtggc agctccgtat cgcgcgcggc
      961 gaagcgctcg ccttcgagca gaaggacatc ggccagcgcg gacacgcgat cgagtgtcgc
     1021 atctacgccg aggacgagcg caagcacttc attccgtcgt gcggcgtcat ctcgctcttg
     1081 cgcgagccgg cgggaccggg ggttcgcgtc gacagcggcg tgtaccaggg atgggaagtg
     1141 acgtcgcact acgaccccat tctcgccaag ctggtcgcgt tcggcgagac ccgcgaggtc
     1201 gcgcggcgac gcatgctgcg cgcgctcgac gaatacgtcg tgcaaggtgt cgcgaccagc
     1261 gtcgacctcc acaaacgaat tctccgccac cccagtttcg tcgcggccaa ggtggatacc
     1321 gcgtttctcg acgagcacgc gaccgacttg ctggagccgg ccaaggccga cgtccccgac
     1381 ctcgcgttca tcgccgccgc gctcgcggcc gagtcgggat ccgggcgcgg cgcgcgcgcg
     1441 acgcacgcgg cgaccaacga cgacgacgcg ccgtcgccgt gggagacgac gggttcgtgg
     1501 gagatcggac gggcgagcta gcatggcgac catcaccatc gacggcgtga gccgagctgt
     1561 gagcgtggcg cgaagcaagg acggattcgt cgtcaccgtg gacggacgtc gctacgacgt
     1621 gcgcgaggtc gcgcgtgtcg ccgaggggat tgcgttcatc gtgggcaacg agtcgcacgt
     1681 ggcgcgcgtg acgagcgggc gcgaggggat cgaactctcg atcggcgccc actcctatgt
     1741 gcaggcgcgg gatctggtcg acgccgaccg ccccgcgggt gcggccgggc gccacggcga
     1801 cggacgcctc gaagccccca tgccgggctc gatcatcggt atccacgttt ccgagggtga
     1861 ccgtgttcgt tccgggcagc cgctggtcgt gctcgagtcg atgaagatgc acaacgagat
     1921 cgcatcgccc atcgatggcg tggtgcgcaa gatcaactgt cgggtcgggg agcaagtcgc
     1981 tttcggccac gtgctggtcg agatcggaac ggaggcgtcg tgaagagtta ctctgcaccg
     2041 tggggaaagc tcctgatcgg gttgaccgcg ttctcgaccc tgatctgcgt cggcgtgtcg
     2101 gtcggggtgg ggctcaatgt gcgcagcatt cctgagaccg aaggcgtcgt gcgcgccgcc
     2161 gccgcgctcc ccatgctcgt ggtcgtgatc gcggccctgt tcatggtgcg cggctacgtc
     2221 gtcaccgccg acgagatcgt cgtgcagcga ccgctgtgga gcagccgctt cgagcgcgct
     2281 cgactgcagt cggctgcgat cgaccccgac gcgctgcgcg gcagcattcg gttgttcggg
     2341 aacggcggct tgttttcctt caccggctgg tttcgaagcc cccaactcgg gcgctaccgc
     2401 gcgtacgtca ccgacccgtc ccgcaccgtg atcctgcgct tcgcggaccg catcatcgtg
     2461 gtgtcgccgt cggacccggc cgcgttcgtg cgcgacgtga ccccgcgcgg atagatacac
     2521 gaacggagac cgcatgcccg acatcgtgct cgccgccatc ctcggcctgg tgcaggccgt
     2581 caccgaattc ctgcccatct cgtcgtcggc gcatttgatc ctcgcgcgcc acgtgctgga
     2641 cttcgaggcc gtcgacggac tcaccttcga cgtcgccctt cacatcggga cgctgctcgc
     2701 ggtcgtggtc tatttctggg gcgatctgcg cgcgctcgcg cgcggttttc tggggagcgt
     2761 cgcgcgccgg agcggccccg ccgaccccgc cggacgactc gcgtggtaca tcatcgcggc
     2821 gtgcgtgccg gccggcttgg tgggattttt cttcgagaag gcaatcgagg gctacttccg
     2881 gaacccgtcc gtgatcgtcg tgacactgct cctgggcgcg tttctgtttc tgtgggtcga
     2941 gcggcgcttc cgtcacgatg gcgagatgca agcgctctcg cttgggcagg cggtggcgat
     3001 cggcgcggcg caatcgctcg cattgatccc cggggtgtcg cgctccggca tcaccatcgt
     3061 ggtcgggatg atgtgcgggc tgcgccgcga ccaggcggcg cgcttctcgt tcctgatggc
     3121 ctcgccgctg atgctggccg cgggggccaa gaaagcgctc gacctggcgg gccagtcgat
     3181 cacgtccgcg cagctcgcgg tgctcgcggt cggtgtggcg acgtccgccg tcgcgggctg
     3241 gctcgtgatt cgatttctgc tcgattatct ccgccgccgc gggctcgacg tgttcgccta
     3301 ttatcgaatc gtcttggcga tcgtggtcgc gatcgtgctg cttgcgtaag aaacgcacgc
     3361 ttgtgtcgcg cccctggttc gcgatagaat aagcgcgccg ctgaccgaca tcgtccggtc
     3421 gtccctccga cgcgaccggc atcccctcac ggggcaggaa ggaagcgcca tgaccggttc
     3481 atctcgccgc ggtctcgcct tttcgctcac cgcctcgctc gctttcacgg tttcgacgtt
     3541 gtccgttcag gccagcgtca agtcgcacca gaagatcagc gacacagccg ggaacttcat
     3601 tgcgccgctc aacgacgacg atcgcttcgg catcggagtg acgccgttgg gcgacctcga
     3661 cggcgacggc gtcgccgata tcgcagtcgg cgcgtacctg gacgacttct acggagatcg
     3721 gtttggagcc gcgtacgtcc tacttctcaa cgccgacggc accgtcaagt cgcacgtcaa
     3781 gataggcatg gggctgggcg gcttcaccgg cgatctggat ttctgggacg agttcggtgt
     3841 ttccgtcacg gcgatcggcg acctcgacga cgacggcgtg atggacctcg cggtgggtgc
     3901 ccgcctcgac gacgactcgc ccagttacgt tgtcgatggg gtcgaccgcg gcgcggtctg
     3961 gatcctgttc ctcaacgcca acgggaccgt gaaatcgcac cagaagatca gcgaccgctt
     4021 cggcggtttt accggagggc tgagcgacgg cgactggttc gggttctaca gcaccgcgct
     4081 cggtgatatc gacggcgacg gcgtcgaaga catcgccgtt tcggcgacgc gagacgacga
     4141 tggcggtacc gatcgcggtg cggtgtggat tctcaacctc caggccaacg ggatggtgaa
     4201 gtctcagtac aagatcagcg gcacccaagg tggattcacc gg
//
LOCUS       DASPGK010000458         9922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1401241, whole genome shotgun sequence.
ACCESSION   DASPGK010000458 DASPGK010000000
VERSION     DASPGK010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401241"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..993)
                     /locus_tag="VEC56_12865"
     CDS             complement(136..993)
                     /locus_tag="VEC56_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXV15091.1"
                     /translation="MNEAVALKAKDYKNDVEPVWCPGCGDFGDLAAFTQALASLQFDP
                     KDICMVSGIGCSGRFSHFVKCYAYHTAHGRALPTAIGVKAANPNLHVFVVGGDGDGFS
                     IGGGHIPHAARRNPDITYMIIDNGIYGLTKGQVSPTSPEGMVSGTTPFGLEDPPLDPL
                     PMLLSYGVSFVARGFSSDVKQVSKLVQAAVAHRGFSIVHLVSPCPTFNQVITFDFMKE
                     RVAPIPDDHDPTSIDAALGLALNRERFFTGVFYRNESLPTMQQKLEHQQRLTMDHQPR
                     SLEKLFAQF"
     gene            complement(1007..2749)
                     /locus_tag="VEC56_12870"
     CDS             complement(1007..2749)
                     /locus_tag="VEC56_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXV15092.1"
                     /translation="MPRSDITVRIAGEGGEGVISTAEILTRAAVDSGLDIFTFRSYPA
                     EIKGGLAMMQVRMSTSPVGSIGRDADVLMAFNQEAFDVWAERMTPGGMLLYDPKHCVV
                     PESFPHARIEVPLHETAMKETGARLAKNIVALAALVGAIDIPPNSARDLIAKQFKRKG
                     DDVVATNLKAFDAGLSLLNGDGRLAVLKLARPSTQGKKSGRVVLSGNQSLSLGAIAGG
                     CRYVAGYPITPATQVLEFMMKHLPAFGGNVLQAEDEIAAIASCLGASFAGVRAMTATS
                     GPGLCLMSELIGMASMSELPVVIVDVMRSGPGTGMPTKTEQADLMYAIHGSAGESPRI
                     VIAATSIEDCFHQAIRAFNLSERYQMPVILLSDQSMGYRTMTMDTPDLAAIRLDARAH
                     PPAESIADYLRFEDTESGVSPIAIPGTAGGMFVTTGLEHDEHGLATYTPENRERMTSK
                     RYRKMDTLASELVERGDGVYDDAGKADIGVIGWGSTEGTIREAIGRLRADGARIAHFH
                     PKVLNPLPVATVEKFLAPLKKVVVLEENHTAQLAQHLRAHVAFNGTEIVTVNQCTGLP
                     FTADEVVAALGSNL"
     gene            2900..3466
                     /locus_tag="VEC56_12875"
     CDS             2900..3466
                     /locus_tag="VEC56_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXV15093.1"
                     /translation="MIRRMQLAILRPMAWLAVAVAGGGGCRRDHGSADAHASYHHAQV
                     VDVAQIEFDDGDTFLYRREPIRVLGIDTPEVAEPDVGIFEDQPYGRAAAESTQALITR
                     AHRVEIAYDGRDIYRRRLAHVFVDGELLGVKLLQMGLAYENVSHFGDNGFPDLADRIL
                     RAAHEGPKPPFEPPYQWRRKNQKKKTNE"
     gene            complement(3468..3815)
                     /locus_tag="VEC56_12880"
     CDS             complement(3468..3815)
                     /locus_tag="VEC56_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV15094.1"
                     /translation="MRHYLLVAVLPVLLSACDGDPVRPENKAPVVTSLSVGLTEVYQG
                     DACPIVCVASDPDGDRLTYEWTVGSGYHTGSGDDIVYVPTACCLGGNPVFVTVRDGRG
                     GVTRAELFIKVTP"
     gene            complement(3933..4568)
                     /locus_tag="VEC56_12885"
     CDS             complement(3933..4568)
                     /locus_tag="VEC56_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15095.1"
                     /translation="MDACIPMEALGTLDTLGPDDPRRRHVERCARCSAMLVAYREFLR
                     ADAPRNAHVGDADTRLAAFIKERIEGIEATAGAPRKPRGRWFEMPSFRFAAAAAVMVV
                     IAGVVAQQMRGPDEPVVRGETRTVVTLDAPRVLADGAIALSWTSVPDADSYQVVLLRD
                     DLSEIVRTPATHEQSLTLDRGSLPSDATRWQVAAFREGAVAAESTPEALPE"
     gene            complement(4608..5195)
                     /locus_tag="VEC56_12890"
     CDS             complement(4608..5195)
                     /locus_tag="VEC56_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV15096.1"
                     /translation="MSHSMKEGGGAPAGTPDRDLVEQAQSHPSSAVGRAAAGELLRRY
                     RRPVYVWCFRYVGEHERALDMAQEVLLSAYQRLGEFEGRAAFSSWLFVIARNRCLNEM
                     RNDARREDIEPDSLVDDGPGFDERLESEMEGQRLLDLAARVLDGDERKAIWLRYSELL
                     PVDEITTLMNLDHKSGARALLQRARRKLRAAWESQ"
     gene            complement(5224..8070)
                     /locus_tag="VEC56_12895"
     CDS             complement(5224..8070)
                     /locus_tag="VEC56_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HXV15097.1"
                     /translation="MSRLVTLIVLVSALAVSAAFPPRSSRHPGPADRTADSLFDAGAY
                     DTMLAFVGRELTRARARGDSLGVGRMTYERGRARQILRVPGGLADLNRAHAIAVTEND
                     SLGWINALGVKSFFAMVDGRLDESLRMNAERIPIAKAIGNTRNEAWGHLLVGYVSLLR
                     ENLPTARSEYEIAATLFRKANRPAQELTALIGLARVLERQGDGAGTKSTYQRALVIAQ
                     ELGDKSQESDVWNNLATMEYEHGDLAVSERYFRRAYLLKRSVDAPDIDAAAANVADIS
                     VLLGSYSAAESVLVDALARSRKWGYTSGVHTLLCELGNVRMAQGRHTGAVSCYRRALS
                     SHGSIENRVEAATGLAAALTAQGDVDAANAALDSHLVDLGRVTPSAWRAAAYVMWART
                     LRAGGNVPRAGRTARAAWDDAASRSDTTGAVVAATELGMCLREAGEVETAYAWFQRAR
                     AMFIARSLRASEFHLREAQRVSLAEPLLELSPVLLDWPREVKRGERERRLFDFIQEIK
                     ARTLLERVADPRRARDIDPSFSIPATATEIQTTVLRPGECLVDFTFAGRNLLAFAVTT
                     EALLMVRIDNADDIQKHVSRYHRLLAQRPAESSEPGDVHAAARSLGMALFGDVAQLVG
                     PSTRVYVATDGWLAALPVETLVCPSDPDVPMLVARDVVRVPSATMLRLQRGRIARPYA
                     TGNYASVLAVASQASELQGARREINRLASRYQNVERLRASDRDAFIEAIGRHDVVHVA
                     SHVRVDAERPWHSGILITDAGPSGAGGERAAGGQETPAPGGVAEASTIDPTRALPVDP
                     YARAAQIAEAQSDARLVVLSGCESALGRATQGEGVLGVAAAFFAAGARSLVASIWEVD
                     DAVTADLMERFYAQLSEGKSVAAALRAAQLDVREDRPHPFYWAGFVVIGDGDVSVRLG
                     EQPAGKRYLFIALIAAVLGVVAWVIVRGRGTIQT"
     gene            complement(8143..8694)
                     /locus_tag="VEC56_12900"
     CDS             complement(8143..8694)
                     /locus_tag="VEC56_12900"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HXV15098.1"
                     /translation="MTRIVVPALTALLCAAPALAQTQATENTFRLDAMENMPKAKLSD
                     VAWIAGWWEGTGIDARAEEVWSEAAGGAMMGMFRLIEEGETVFYELMTIAETDGSLTL
                     VLKHFNDDLTGWEEHDEVVEFPLVRLSDDEAVFDGLTYRRKGDKGLHVFVLVAGDDGT
                     LEEMEFTYDRASGPPAGKGRRSR"
     gene            complement(8748..9272)
                     /locus_tag="VEC56_12905"
     CDS             complement(8748..9272)
                     /locus_tag="VEC56_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV15099.1"
                     /translation="MNTQTEVWLRGPVYGIPPLLMPVAHALLQAKEDAARAAEGLSDE
                     ALWTRPGGAASAGFHLFHMAQSLDRLMTYARGETLTDSQRVALALEKSGEIDAGADDL
                     IELVATSVERALRQLRCTSEAALSGERRIGRSMVPSTVLGVLFHAAEHTTRHAGQLIT
                     TIKVVTGRHDPREE"
     gene            complement(9358..>9922)
                     /locus_tag="VEC56_12910"
     CDS             complement(9358..>9922)
                     /locus_tag="VEC56_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXV15100.1"
                     /translation="GYILIGMAALGIDNTKAASGVMFYLVVYAFMTLGAFALISALEN
                     REDSRGLELEDFSGLGLRRPVLGAVMALFMFAMAGIPPTAGFFGKYYIFSAAIDRGLT
                     WLAVVGVLNSALSLYYYLRVVVYMYMRKPKTDPTPVFDDWGIRTVLFVSVLVTIWLGL
                     GPSGVVPGIETVLDWTSDSLDRIASLR"
BASE COUNT         1683 a         3321 c         3318 g         1600 t
ORIGIN      
        1 aagcgacgag gacttttccg aacggtgacg atagaaacgt gtacgaaatc gacatggctc
       61 ctccttcacg tcgctccatg gatacgcgac gtgtcgggtg cttcgccacc gcggaatgaa
      121 tgcggaattc ccggcctaga actgggcgaa gagcttctcg agcgaccggg gctggtggtc
      181 catcgtgagc ctttgctggt gctcgagctt ttgctgcatt gtcggcaggc tctcgttgcg
      241 gtagaacacg cccgtgaaga aacgctcccg gttcaacgcc aggccgaggg ccgcgtcgat
      301 gctcgtggga tcgtggtcgt cggggatcgg tgcgacccgt tccttcatga aatcgaacgt
      361 gatcacctga ttgaacgtcg ggcagggcga aaccaaatgg acgatcgaga atccccggtg
      421 cgcgaccgcc gcttgcacga gcttcgacac ctgcttgaca tcggacgaaa aaccgcgcgc
      481 gacgaagctc acgccgtagg acaggagcat cggcaggggg tcgagcggcg ggtcctcgag
      541 tccgaacggg gtagtgcccg aaaccatgcc ttccggcgag gtcggcgaga cctgcccctt
      601 ggtgaggccg tagatgccgt tgtcgatgat catgtacgtg atgtcggggt tgcggcgcgc
      661 cgcgtgcggg atgtggccgc cgccgatcga aaagccgtct ccgtcgccgc ccaccacgaa
      721 cacgtgcaga ttcgggttgg ccgccttgac gccgatcgcg gtcgggagcg cgcggccgtg
      781 cgcggtgtgg tacgcgtagc acttgacgaa gtgggagaag cgccccgagc acccgatgcc
      841 cgacaccatg cagatgtcct tggggtcgaa ttgcagcgag gccagcgcct gggtgaacgc
      901 ggccaggtcg ccgaaatcac cgcacccggg acaccaaacc ggctcgacgt cgttcttgta
      961 atccttggcc ttgagggcga cggcttcgtt catgatgcgg attcctttat agattgctcc
     1021 cgagcgccgc cacgacttcg tcggcggtga acgggagacc cgtgcactgg ttgacggtga
     1081 cgatctcggt gccgttgaag gcgacgtgcg cgcgcaggtg ctgcgcgagc tgcgcggtgt
     1141 gattctcttc cagcacgacg actttcttga gcggtgcgag aaacttctcg acggtcgcca
     1201 ccggaagcgg gttgagaacc ttgggatgga agtgcgcgat gcgggcaccg tcggcgcgca
     1261 gccgcccgat cgcttcgcga atcgtaccct cggtggaacc ccagccgatg acgccgatgt
     1321 cggccttccc ggcgtcgtcg tagacgccat cgccacgctc gacgagctcg ctcgcgagcg
     1381 tgtccatctt gcggtagcgc ttgctggtca tacgctcgcg attctccggc gtgtaggtgg
     1441 caaggccgtg ctcgtcgtgc tcgaggccgg tggtcacgaa catgccgccc gccgttcccg
     1501 ggatcgcgat cggcgagacg ccgctctccg tgtcctcgaa tcgcaggtaa tccgcgatcg
     1561 attcggcggg cggatgggcc cgggcatcga gacgaatcgc ggccaggtcg ggcgtatcca
     1621 tggtcatagt gcggtagccc atcgattggt cgctcaacag gatcaccggc atctggtagc
     1681 gctcggacag attgaaggcg cgaatggcct ggtggaaaca atcctcgatg ctggtggccg
     1741 cgatcacgat gcgcggcgat tcaccggcac ttccgtggat cgcatacatg agatccgcct
     1801 gctcggtctt ggtgggcatg ccggtgccgg gaccgctgcg catcacgtcg acgatgacga
     1861 cgggcaattc cgacatcgac gccatgccga tcaactcgct catgaggcac aaccccgggc
     1921 ccgacgtcgc ggtcatggcg cgcacgccag cgaacgacgc ccccagacac gacgcaatgg
     1981 ccgcgatctc gtcttcggct tggaggacgt ttccgccgaa cgcgggcagg tgcttcatca
     2041 tgaactcgag cacctgggtg gcgggcgtga tcgggtaacc ggccacgtag cggcagccgc
     2101 ccgcgatcgc gcccagactc aatgattgat tgcccgacag cacgacgcgc ccgctcttct
     2161 ttccctgcgt ggacgggcgc gcgagcttga gtaccgccaa gcggccgtcg ccgttcaata
     2221 gcgagagccc cgcatcgaag gccttcaagt tggtggcgac gacgtcgtcg cctttgcgct
     2281 tgaactgctt cgcgatgagg tcgcgcgcgc tgttgggggg aatgtcgatg gcacccacca
     2341 gcgccgcgag cgccacgatg ttcttcgcga ggcgcgcgcc cgtctctttc atcgcggtct
     2401 cgtggagcgg gacttcgatg cgcgcgtggg ggaagctctc gggaacgacg cagtgcttgg
     2461 gatcgtagag aagcatccca cccggggtca tccgctcggc ccacacgtcg aaggcctcct
     2521 ggttgaacgc catgagcacg tcggcgtcgc ggccgatgga ccccaccggc gaggtgctca
     2581 tgcgcacctg catcatcgcg aggcccccct tgatctcggc gggataggaa cggaacgtga
     2641 agatatcgag gcccgaatcg acggcggcgc gggtcagaat ctccgcagta ctaatgacgc
     2701 cctcgccgcc ttcgccggcg atgcggacgg taatgtcgct gcgcggcatg ttgctcctgt
     2761 ggccgcggtc cgtggccacg aaggcagtat aacagacgag cacccggtcc gctaccgcca
     2821 gcaccgaccg tcgagcctgc ccgccccatg gggaaaacgg gccgcgcgaa gccgttgcga
     2881 ggcaccatcg gaatcgacga tgattcgccg catgcaactc gccattcttc gccccatggc
     2941 ctggctcgcg gttgcggtcg cggggggggg cggctgtcgc cgcgatcacg gcagcgcgga
     3001 cgcccacgct tcctaccacc acgcccaagt cgtcgacgtc gcccagatcg agttcgacga
     3061 cggcgacacc ttcttgtatc gccgcgagcc aattcgcgtc ttgggcatcg acacccccga
     3121 ggtggccgag cccgatgtcg gcatcttcga ggatcaaccg tacgggcgtg cggccgccga
     3181 gtcgacgcag gcactcatca cgcgcgcgca ccgagtcgaa atcgcgtacg acggccgcga
     3241 tatctaccgg cgccgcctgg cgcacgtatt cgtcgatgga gagctattgg gagtgaagct
     3301 cttgcagatg gggctggcgt acgagaatgt gagccacttc ggcgacaacg gattcccgga
     3361 cctggcggac cgtatcttgc gcgccgcgca cgagggaccc aagccgccct tcgagccgcc
     3421 ctaccagtgg cgtcgaaaga atcagaagaa gaagacgaac gagtaggcta cggcgtaact
     3481 ttaatgaaca actcggcgcg cgtgacaccg ccgcggccgt cgcgcacggt gacgaagacc
     3541 gggttgccgc ccaggcagca cgcggtcgga acgtagacga tatcgtcgcc gctgccggtg
     3601 tggtatccgg agccaaccgt ccattcgtac gtgaggcggt cgccgtccgg gtccgaggcg
     3661 acgcacacga tcgggcaggc gtcgccctga tagacctccg tcaacccgac cgaaagcgac
     3721 gtgaccacgg gcgccttgtt ctcggggcga accgggtcgc cgtcacaggc cgatagcaga
     3781 accggcagca ccgcgactag cagatagtgt ctcatgacgt taccaacgat accgcacgac
     3841 ggttggatcg gcaataccta tgatcggcga agaaaagggc cgggcgatga ccgcccggcc
     3901 cgtcgttcca gcgcttcctc ggcccttgat ggttattcgg gcagtgcctc gggggtcgat
     3961 tccgccgcca ccgcgccctc tcgaaacgcg gccacctgcc agcgcgttgc gtcgctcggg
     4021 agcgagccgc gatcgagcgt cagggactgc tcgtgcgtcg cgggggtgcg aacgatctcc
     4081 gaaagatcgt cccgcaagag gacgacctgg tacgaatcgg cgtcgggcac ggaagtccag
     4141 ctcaacgcga tggccccgtc cgccagcacg cgcggtgcgt ccagcgtgac cacggtgcgc
     4201 gtctcgcctc gcaccaccgg ctcatccgga ccccgcatct gctgggccac gactcccgcg
     4261 atcaccacca tcaccgcggc agccgccgcg aatcgaaagg atggcatctc gaaccagcgg
     4321 ccgcgcggct tgcgcggagc acccgcggtc gcttcgatac cctcgatgcg ctctttaatg
     4381 aaggctgcca agcgcgtgtc ggcgtcgcca acgtgcgcgt tgcgcggggc atcggcgcga
     4441 aggaactccc tgtacgcgac gagcatcgcg ctgcatcgcg cgcagcgttc gacgtgtcgc
     4501 cgcctaggat cgtcgggacc gagcgtatcc agcgtcccca gcgcttccat ggggatgcaa
     4561 gcgtccatcg tgtctcctcc ctcgttgcca gccacccggc ccgtgcgtta ttgcgattcc
     4621 caggcggccc gcagcttgcg tcgcgcgcgc tgcaggagtg cccgcgcgcc cgacttgtga
     4681 tccaaattca ttagcgtggt gatctcgtcc accggcaaga gctccgaata ccggagccat
     4741 atggccttgc gctcgtctcc atcgaggacg cgcgcggcga gatccaacag tcgctgaccc
     4801 tccatctccg attcgagccg ctcgtcgaaa ccgggcccgt cgtccaccaa cgaatcgggc
     4861 tcgatgtctt cgcgccgcgc gtcgttgcgc atttcgttca ggcagcgatt gcgtgcgatc
     4921 acgaacagcc aggacgaaaa ggcggcgcgt ccctcgaact cgcccaggcg ttggtacgca
     4981 gagaggagca cctcttgggc catgtcgagg gcccgctcgt gctcgccgac gtaccgaaag
     5041 caccagacgt agaccggacg tcggtaccgt cgcaagagct ccccggccgc ggctcggccg
     5101 acagcactgc tcgggtgcga ctgggcctgc tcgaccaggt cccggtccgg ggtgccggcc
     5161 ggagccccgc cgccttcctt cattgagtga gacatagaac gcccttagcg atcacacggt
     5221 aattcacgtc tgtattgtac cgcgccccct cactattacc caggcaacca cgccgaggac
     5281 ggcggcaatc agcgcaatga agaggtagcg cttcccagcc ggctgctcgc ccaggcggac
     5341 cgagacgtca ccgtccccga tcaccacaaa cccagcccaa taaaagggat gcgggcggtc
     5401 ttcgcgcacg tcgagctggg ccgcgcggag ggccgccgcg accgacttcc cttcggagag
     5461 ctgggcgtag aatctttcca tgaggtcggc ggtcaccgca tcgtcgacct cccagatgct
     5521 cgccacgagc gatcgggccc cggcggcaaa aaaagccgcg gcgactccca gcaccccttc
     5581 gccctgggtc gcccgcccga gcgccgattc gcaccccgag agaaccacca ggcgcgcgtc
     5641 gctctgtgct tcggcgatct gggcggctcg ggcgtacggg tcgaccggaa gcgcccgggt
     5701 cgggtcgatg gtgctcgcct ccgccacccc gcccggggcc ggagtttcct gccctcccgc
     5761 cgcacgctcg ccgccggctc cgctcggacc ggcatcggtg atcagaattc cggagtgcca
     5821 gggccgctcg gcgtccacgc gcacgtgcga agccacgtgc acgacgtcgt ggcgcccgat
     5881 cgcttcgatg aaggcgtcgc ggtcggacgc gcgcagacgc tcgacgttct ggtagcgcga
     5941 cgccagccgg ttgatctcgc ggcgggcacc ctgcagttcg ctcgcttgtg atgcgacggc
     6001 cagcacggag gcgtagttcc cggtcgcgta cgggcgcgcg atgcgccccc gctggagccg
     6061 gagcatggtg gccgacggca cccgcaccac gtcgcgcgcg accagcatgg gaacgtcggg
     6121 gtcgctcggg cacaccaacg tctccacggg aagggccgcc agccatccgt cggtggcgac
     6181 gtatacccgg gtcgacggac ccaccagctg cgcgacgtcg ccgaagagcg ccatcccgag
     6241 cgaacgcgct gcggcgtgca cgtcgcccgg ctcggaggac tcggccggtc gctgcgccag
     6301 gagccggtgg tagcggctca cgtgcttctg gatatcgtcc gcattgtcga tcctcaccat
     6361 gagcagggcc tcggtggtca ccgcgaacgc gagcaagttg cgacccgcga atgtgaaatc
     6421 gaccagacac tcgcccgggc ggagcacggt ggtctgaatc tcggtcgcgg tcgctggaat
     6481 cgagaagctc gggtcgatgt cgcgcgcgcg ccgcgggtcg gcaacccgct cgagcagggt
     6541 gcgcgccttg atctcctgga tgaagtcgaa gagccggcgc tcgcgttcgc cgcgcttgac
     6601 ctcacgcggc cagtcgagca atacggggct caactccaac agaggttcgg ccagcgacac
     6661 gcgctgcgct tcgcgcagat gaaactcgct cgcgcgcagc gagcgcgcga tgaacatcgc
     6721 gcgggcgcgc tggaaccacg cgtacgccgt ctccacttcc cccgcctcgc gcaggcacat
     6781 gcctagctcg gtcgcagcca ccacggctcc cgtggtgtcc gatcgcgacg cggcgtcgtc
     6841 ccaggccgcg cgcgcggtgc gccccgcgcg cggaacattc ccgccggcgc gcagcgtgcg
     6901 cgcccacatc acgtaggccg cagcgcgcca tgccgagggc gtcacgcgcc ccaaatcgac
     6961 caagtgggag tcgagggccg cgttcgcggc gtcgacgtcg ccctgcgcgg tcaacgcggc
     7021 cgcgagcccg gtcgccgctt cgacgcgatt ctcgatcgag ccgtgcgacg acaacgcgcg
     7081 ccggtagcac gacaccgcgc ccgtgtggcg cccttgcgcc atgcggacgt tacccagctc
     7141 gcacaatagg gtatggacgc ccgaggtgta gccccacttg cgcgagcgcg cgagcgcatc
     7201 caccagaacc gactccgccg ccgagtagct gccgagcagg acgctgatat cggcgacatt
     7261 cgcggccgcg gcgtcgatgt cgggggcatc gaccgaacgc ttgagcaggt acgcacggcg
     7321 gaagtagcgc tcgctgaccg ccaagtcgcc gtgctcgtat tccatggtcg cgaggttgtt
     7381 ccacacgtcg gattcttggc tcttgtcgcc gagttcctga gcgatcacga gcgcgcgctg
     7441 gtaggtcgat ttcgtcccgg cgccatcgcc ctggcgttcg agcacgcgcg cgaggccgat
     7501 caacgccgtg agttcttggg ccggtcgatt ggctttgcga aagagcgtgg ccgcgatttc
     7561 gtactcggaa cgcgcggtgg gcaggttctc gcgcaggagg ctcacgtagc cgaccagcag
     7621 gtgaccccac gcctcgttgc gcgtgttccc gatcgccttc gcgatcggaa tgcgctcggc
     7681 gttcatgcgc agcgattcgt ccaggcggcc gtcgaccatg gcgaagaagc tcttcacgcc
     7741 cagcgcgttg atccacccca gggagtcgtt ctcggtgacg gcgatcgcgt gggcgcggtt
     7801 gagatccgcc agcccgcccg ggacgcgcag gatctgccgg gcgcggccgc gctcgtaggt
     7861 catgcggccg acccccagcg agtcgccgcg ggcccgcgcg cgcgtgagct cgcgcccaac
     7921 aaatgcgagc atggtgtcgt acgcgccggc gtcgaagagc gaatcggcgg tgcggtcggc
     7981 ggggccgggg tggcgagagg aacgcggcgg aaatgcggcg gaaaccgcaa gcgccgacac
     8041 caaaacgatc agcgtgacca gtcgggacat gggctcgggc ttcgagatcc tccttggggt
     8101 caccctaccg gaagtaggcg caacccggcc agagagaact tgttaccggg accgccgccc
     8161 cttgccggcc ggggggccgc tcgcgcgatc gtacgtgaac tccatctcct cgagggtacc
     8221 gtcgtcgccg gccacgagca cgaacacgtg gaggcccttg tctcccttgc ggcggtaggt
     8281 gaggccgtcg aagacggcct cgtcgtcgga taagcgaacg aggggaaact cgaccacctc
     8341 gtcgtgttct tcccacccgg tgagatcgtc gttgaagtgc ttgaggacca gggtgaggct
     8401 cccgtcggtc tccgcgatgg tcatgagctc gtagaagact gtctcgccct cttcgatcaa
     8461 tcgaaacatc cccatcatcg cgcctccggc ggcttcgctc cagacctcct cggcgcgcgc
     8521 gtcgatgccc gtcccctccc accaccccgc aatccacgcc acgtcggaga gtttcgcctt
     8581 gggcatgttc tccatcgcgt cgagccggaa cgtattctca gtcgcctggg tttgcgcgag
     8641 ggcgggcgcg gcgcaaagca gcgccgtgag ggcgggaacc actatccgag tcattcgaga
     8701 gcctccttgg cccgtgagtg tacgggcggg gggcgacggt tgcagcgcta ttcttctcgg
     8761 gggtcgtgtc ggccggtgac gactttgata gtcgtgatga gctggccggc gtggcgggtg
     8821 gtgtgctcgg cggcatggaa cagaacgccc aggaccgtcg agggcaccat cgagcgcccg
     8881 atccggcgct cgccggagag cgcggcctcg gaggtgcatc gcagctggcg aagcgctcgc
     8941 tcgacggacg tcgccaccag ctcgatcaag tcgtcggcgc cggcatcgat ctcgccgctc
     9001 ttttccaggg cgagtgcgac ccgctgcgaa tccgtgagcg tctccccgcg cgcgtaggtc
     9061 atgagccggt cgagcgactg ggccatatga aacagatgga agccggcgga cgcggcaccc
     9121 cccgggcggg tccagagggc ttcgtcggag agcccttcgg cggcgcgcgc ggcgtcctcc
     9181 ttcgcttgga ggagggcgtg ggcgaccggc atcaacaacg gcgggatgcc gtagacgggg
     9241 ccgcggagcc agacttcggt ttgggtattc ataaggagca accggcctcc ttcgcgcttt
     9301 tcgacgcgac ggaggccggg ttccactaag acagtgtagt cggattcgcg cgtgcggcta
     9361 ccgaagactc gcgattcggt ccagcgaatc gctggtccag tcgaggacgg tctcgatgcc
     9421 gggcaccaca ccgctcgggc ccaggcccag ccagatggtc acgagcaccg agacgaagag
     9481 gaccgtgcgt atgccccagt cgtcgaagac gggagtcggg tcggtcttgg gcttgcgcat
     9541 gtacatgtag acgacgacgc gcaggtagta atagagggag agagcgctgt tcaacacacc
     9601 gaccacggcg agccacgtga gcccgcggtc gatggcggcg gagaagatgt aatacttgcc
     9661 gaagaagccc gccgtgggcg gaatgcccgc catcgcgaac atgaagagcg ccatcacggc
     9721 gccgagcacg ggccggcgca ggcccagccc cgagaaatcc tccagctcca ggccccgcga
     9781 gtcctcgcgg ttctcgagcg ccgagatgag cgcgaaggcg cccaaggtca taaaggcgta
     9841 cacgaccagg tagaacatga cgcccgacgc ggccttggtg ttgtcgatgc cgagcgccgc
     9901 catgccgatc aagatgtagc cc
//
LOCUS       DASPGK010000459         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_466744, whole genome shotgun sequence.
ACCESSION   DASPGK010000459 DASPGK010000000
VERSION     DASPGK010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466744"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..848)
                     /locus_tag="VEC56_12915"
     CDS             complement(63..848)
                     /locus_tag="VEC56_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV15101.1"
                     /translation="MLQANTRADIYESHRRELVNYATRLVVRESVAEELAQEAALRLL
                     QDKGLPNDEGQIRAWLFRVVSNLAIDHLRRHSTWKELVLVDARERAVADPTFVAESQQ
                     MRSSPELAAIAREHLAVCFACTLRNLPPQQAAALLLREVYGFSTAETSESLDATVVQV
                     KNWIQQARRTMEERYAHTCALVTKQGVCHQCVELDGFFNGRSRDPLDGTARNLEARLA
                     ILRETREAALGPWHRRMLRLVDEVLEGGPAGERALGSVRPESE"
     gene            complement(867..1394)
                     /locus_tag="VEC56_12920"
     CDS             complement(867..1394)
                     /locus_tag="VEC56_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV15102.1"
                     /translation="MARRLTAEGARVFLAGRTESALKKLAAELHAPWQRVDGTDETAV
                     TVWVDRIARQADGIDVMFNAIGPRPIDADYATPSTTISYERFALPLNLIVGSQFLTAR
                     AVARHMVPRGRGAIVFLSASLTGQFIPFMSGITAACGAVEGLSRTLATEFGPAGVRVN
                     YVRAGGPETRRIVSR"
     gene            complement(1440..2255)
                     /locus_tag="VEC56_12925"
     CDS             complement(1440..2255)
                     /locus_tag="VEC56_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15103.1"
                     /translation="MSGEALLDRCLAAHGGLDRWRQFEAIDVDVTTGGLAFAAHVQPS
                     LRTTARVATREPLVVLSPYRRWPRGVFQRDRVFIEDASGRVMSERKNPRARMGRGRRL
                     LWWDTLDTLYFVGYALSNYVRSPFLFCQGGFHVASAGVATFGSETWSKLRVTFPPDEP
                     THCRTQAYYLDAMHLIRRVDYVAEPIGRWARAAHFCTGYREWRGLRVPTDRWVVPRTR
                     DDRVWSWPPLVQIHVTAVRPIRGAAISNRCARAHLCDVPRPLVAASAQHGVAR"
     gene            complement(2245..>2782)
                     /locus_tag="VEC56_12930"
     CDS             complement(2245..>2782)
                     /locus_tag="VEC56_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV15104.1"
                     /translation="ATNVLTDTGIPLPLQLVRPPVVGDVFARLLFGRTGLSMMWFAAV
                     ARRDRFSLADYRAILRFPQGITSTRRLFQASLRDLPGLYGPVHAALSGIQVPCTVVWG
                     DRDPFFPTAVGERTAAHIPGARFVQLKGCGHFLPAEDPGGFARVVEELVSGSTLTNGH
                     VPVGSQGGSTAPVVANVG"
BASE COUNT          534 a          944 c          864 g          440 t
ORIGIN      
        1 tcacccatcg atcgccggtc ggcatctggg ctcgatcaac acaatccatg cttcatccac
       61 acctactcgc tctcgggccg tacggaaccg agggcgcgct cgccggccgg gccaccctca
      121 agcacctcat cgacgagccg caacatgcgc cgatgccagg ggccgagcgc ggcctcgcgg
      181 gtctccctga ggatggcgag ccgagcttcg aggttgcgcg cggtgccgtc gagaggatcg
      241 cgactccgcc cgttgaagaa accgtcgagc tccacgcact gatgacacac accttgcttc
      301 gtcacgagag cgcaggtgtg cgcgtaccgc tcctccatgg ttcggcgcgc ctgctggatc
      361 cagtttttca cttggacgac ggtcgcatcg agcgactcgg acgtctccgc ggtcgagaag
      421 ccgtaaactt cgcgcaacag caaggcggcc gcctgctgcg gcggcaggtt acgcagcgtg
      481 caggcaaaac acacggccaa gtgctctcgc gcgatagccg ccaactccgg tgagctgcgc
      541 atctgctgcg attcggcgac aaatgtggga tccgcgaccg cgcgctcgcg cgcgtcgacg
      601 agcaccagtt ccttccacgt actatgtcga cgtaggtgat cgatcgcgag attgctgacg
      661 acgcggaaca gccacgctcg gatctgacct tcgtcgttgg gcaacccctt gtcttggagg
      721 aggcgcaacg cggcctcctg cgccagctcc tcagcgacgc tctcccgtac aaccaaccgg
      781 gtcgcgtagt tcacgagttc gcgacgatga ctctcgtaga tgtcggcgcg cgtgttggcc
      841 tgcagcacgg cttcacctcg ctttcgctat cgtgacacga ttcggcgcgt ctcgggccct
      901 cccgcgcgga cgtagttcac gcgcacgccc gcggggccga actctgtcgc caaggttcgg
      961 ctgagcccct cgacggcgcc gcaggcggcg gtgatccccg acatgaacgg aatgaactgc
     1021 ccggtcaggc tcgccgacag gaacacaatg gcgccgcgtc ctcggggaac catgtgccga
     1081 gccacagccc gtgccgtcag gaactgcgaa ccgacgatca gattcagcgg aagcgcaaac
     1141 cgctcgtacg aaatggtggt ggatggcgtc gcatagtcgg cgtcgatcgg cctcggaccg
     1201 atcgcgttga acatcacatc aatgccgtcc gcctggcggg cgattcgatc gacccacacc
     1261 gtgacggcgg tttcatcagt accatccacg cgctgccacg gcgcgtgaag ttccgccgcc
     1321 agcttcttga gtgccgactc cgtgcgaccg gccaggaaca cgcgtgcgcc ctcagccgtc
     1381 aaccgccgag ccaatagcgc cgttcgcgcc gagaatcaga gcgtttttgt tgtgcaggat
     1441 taccgtgcaa ctccatgctg agcgctggcg gctaccaacg gtcgaggaac gtcacacaga
     1501 tgcgcgcgtg cgcaacgatt cgaaatcgct gcgccacgga tcggtcggac ggctgtgaca
     1561 tgaatctgca cgagtggcgg ccaggaccac acgcgatcgt cacgcgtgcg aggcacgacc
     1621 catcggtccg tgggcacacg caggccacgc cactcgcgat agcccgtaca gaaatgcgcc
     1681 gcgcgcgccc agcggccgat cggctcagcc acataatcga ctcgtcggat caagtgcatc
     1741 gcgtcgaggt agtacgcctg ggtgcggcag tgcgtgggct cgtccggcgg aaacgtcacg
     1801 cgaagtttcg accacgtctc gctgccgaac gtagcgacgc cggccgacgc gacgtggaag
     1861 ccgccctggc agaacaggaa cggtgaacgc acatagttcg agagcgcgta cccgacgaaa
     1921 tacagcgtgt cgagcgtatc ccaccagagg agacgacgtc cgcgacccat gcgcgcgcgt
     1981 gggttcttcc gctcggacat cacacggccg gacgcgtctt cgatgaagac acggtctctc
     2041 tggaagacgc ctcggggcca tcgccgatat ggtgacaaca caaccagtgg ctcccgcgtt
     2101 gccactcgtg ccgtcgtgcg cagcgatggc tgtacatgcg ccgcgaaggc caggccaccg
     2161 gttgtgacgt cgacgtcgat agcctcgaac tgccgccatc ggtcgagccc tccgtgcgcg
     2221 gcgagacacc gatcgaggag cgcttcaccc gacattggcg acgaccggcg cggtcgagcc
     2281 cccttggctc ccaactggca catgaccatt cgtcaacgta ctcccggaca caagctcctc
     2341 caccactcga gcgaagccac ccggatcctc cgctggcaag aagtgaccac accctttcaa
     2401 ctgcacaaag cgagcgccgg gaatgtgcgc ggccgttcgc tcaccaacgg cagtcgggaa
     2461 gaacggatcg cggtctcccc acaccacggt gcacgggacc tggatcccac tgagggcggc
     2521 gtgtactggc ccgtataggc ccggcaggtc gcgaaggctc gcctggaaga gacgccgcgt
     2581 cgacgtgatg ccctgaggaa accttaggat cgcgcgatag tcggcgagac tgaatcgatc
     2641 gcgtcgcgcg accgcagcga accacatcat agagagaccc gtgcgcccga acaagagtct
     2701 ggcgaacacg tcgccaacca ccggtggccg gaccagctga agcggaagcg gaatgccggt
     2761 atccgtgagc acgttcgtgg cc
//
LOCUS       DASPGK010000460         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_701590, whole genome shotgun sequence.
ACCESSION   DASPGK010000460 DASPGK010000000
VERSION     DASPGK010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701590"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VEC56_12935"
     CDS             <1..603
                     /locus_tag="VEC56_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXV15105.1"
                     /translation="IWMANADALRVYDALFSAGHAYGLEPAGLDAMDVTRVEAGFILN
                     GVDYYSALRCMIPSRQSTPFELGLGWMVNTEHGCIGSEALAIERELGPIRKLVGLDID
                     WNETEALFRAHRLPPEVHPGAWRDPRPVYDARGAFIGQATSGAWSPLLKKNLALATLV
                     REHTAPGTEVRFEVTVEYERRTVKATVVETPFFNPERKRA"
     gene            626..2212
                     /locus_tag="VEC56_12940"
     CDS             626..2212
                     /locus_tag="VEC56_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV15106.1"
                     /translation="MAKYDAIIVGAGHNGLVAAAYLAKAGRRVLVVEQRPVIGGAAVT
                     EEVFPGFKFSVCSYVVSLFRPHIIRELELTKHGMQLIPLECSFTPHLEGPGLVRWADA
                     NRTRREVSRFSRKDAEIYPEFALAMSKMSFFVKHIIDSPPPDVGSLHPRELSQLSRLF
                     GHLRDLGPDMMELHAQLLTMSGVDFLKMWFESDALLAPMSCSGIIGTFLGVHSPGTAY
                     VLLHHYMGEIDGAFRSWGFSKGGTGGVSQAIANAAIHHGATIRTSAPVARVVVKHGRA
                     KGVVLENGEEIDAKVVISNADPRRTFLTMVGENHFDASFTEQVKRYKYRGSSGKVNLA
                     LDRFPDFTCRPGDGVHVRGDIAVAPSLEYLERAYDDAKYGEFSKRPYLNVVVPSLVDP
                     SVAPPGKHVMSIFVQYAPYHLKAGAETWPKRREEFGDTVVDTLAEYCPGLKDSILHRQ
                     VLTPWDLEHEIGLTEGNIFQGELSLEQLLFLRPVQGWSRYRTPVRGLWLCGSGAHPGG
                     GIMGAPGELCAKSLMNEGEV"
     gene            2214..>2573
                     /locus_tag="VEC56_12945"
     CDS             2214..>2573
                     /locus_tag="VEC56_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV15107.1"
                     /translation="MATRYDVIVIGAGHNGLTTACLLARAGRRVLVLERRATVGGLCA
                     PEEFHPGYHAPGILHDTAQLRTELLHDLHLDEHGLDLAPPESTLFAAPDGGGVLIQTA
                     DPVATAAEIARVSASDAR"
BASE COUNT          413 a          886 c          860 g          414 t
ORIGIN      
        1 atctggatgg ccaacgccga cgcgctgcgc gtgtacgacg cgctgttctc cgcgggccac
       61 gcctacgggc tcgagcccgc cgggctcgac gccatggacg tcacccgtgt cgaagcgggc
      121 ttcattctca acggcgtcga ctactactcc gcgcttcgct gcatgattcc gtcgcggcag
      181 tccacaccct tcgagctggg cctgggctgg atggtgaaca cggagcacgg gtgcatcgga
      241 agcgaggcac tcgcgatcga gcgcgagctg gggccgattc gcaagctggt cggtctcgac
      301 atcgattgga acgagaccga agcgttgttc cgagcgcacc gtctgccgcc cgaagttcac
      361 ccgggtgcct ggcgcgaccc gcgaccggtg tacgacgcgc gcggcgcctt catcggtcag
      421 gcaaccagcg gcgcgtggtc gcccttgttg aagaagaacc tcgcgctcgc gaccctcgtg
      481 cgcgagcaca ccgcgcccgg gaccgaagtc cgcttcgaag tgaccgtcga gtacgagcgc
      541 cgcaccgtca aggcgacggt ggtcgaaacg ccgttcttca accccgaacg aaagcgcgcg
      601 taggcgcgcc cgtcgaccca gccacatggc gaaatacgac gcgatcatcg tgggcgccgg
      661 tcacaacggc ctcgtcgccg ccgcctacct cgcgaaggcc ggccgccgcg tcctcgtggt
      721 ggagcagcgc cccgtgatcg gcggcgccgc cgtcaccgaa gaagtctttc ccggcttcaa
      781 gttctcggtg tgctcgtacg tggtgagcct gttccgcccg cacattatta gagagctcga
      841 gctcaccaag cacggcatgc aattgatccc gctcgagtgt tcgttcacgc cgcacctcga
      901 agggcccgga ctcgtgcgct gggccgacgc caatcgcacg cgccgcgaag tctcgcgctt
      961 cagtcgcaag gacgcggaga tctacccgga gttcgcgctc gcgatgagca agatgagctt
     1021 cttcgtgaag cacatcatcg actcgccgcc ccccgacgtg ggctcgctcc acccgcgcga
     1081 gctgtcgcag ctgtcgcgac tcttcgggca cctgcgcgac ttgggccccg acatgatgga
     1141 gctgcacgcg cagctcttga ccatgagcgg cgtcgacttc ctgaagatgt ggttcgagtc
     1201 cgacgccttg ctggcaccca tgtcgtgcag cgggatcatc gggacattcc tgggcgtgca
     1261 ctcgccgggc accgcgtacg tgctcctgca ccactacatg ggggagatcg acggcgcctt
     1321 tcgctcgtgg ggattctcga agggtggtac cggcggggta agccaggcca tcgcgaacgc
     1381 ggcgattcat cacggcgcca ccattcgcac cagcgcgccg gtggcgcgcg tcgtggtgaa
     1441 gcacggccgc gcgaagggcg tcgtcttgga gaacggggaa gagatcgacg cgaaggtcgt
     1501 catctccaac gccgacccgc ggcgcacgtt cttgaccatg gtgggcgaga accacttcga
     1561 cgcgtcgttc accgagcagg tcaagcgcta caagtaccgg ggcagctcgg gcaaggtgaa
     1621 cctggcgctc gaccgcttcc cggatttcac ctgccggccc ggcgacggcg tccacgtgcg
     1681 cggcgacatc gcagtggcac cctcgttgga gtacttggag cgcgcctacg acgacgccaa
     1741 gtacggcgag ttctcgaagc ggccctatct caacgtggtc gtcccgtcgc tcgtcgaccc
     1801 gagtgtggcg ccgccgggca agcacgtgat gtcgatcttc gtccagtacg cgccctatca
     1861 cctcaaggcc ggcgcggaga cgtggcccaa gcgccgcgag gagttcggcg acaccgtggt
     1921 cgacacgctg gccgagtact gcccgggctt gaaagactcg atcctccacc gccaggtgct
     1981 gaccccgtgg gacctcgagc acgagatcgg cttgaccgag ggcaacatct tccagggcga
     2041 gctgtcgctc gaacagctct tgttcctaag gcccgtgcag ggctggtcgc gctaccggac
     2101 cccggtgcgg ggtctctggc tgtgcggctc gggcgcgcac ccgggcggcg gcatcatggg
     2161 ggcgcccggc gagctgtgcg cgaagtcgct catgaacgag ggggaggtgt agggtggcga
     2221 cgcgctacga cgtcatcgtg atcggcgcgg ggcacaacgg cctcacgacc gcctgcctgc
     2281 tggcccgcgc cggaaggcgc gtcctggtgc tggagaggcg cgcgacggtc ggcgggttgt
     2341 gcgcgcccga ggagttccac cccggctatc acgcgcctgg cattctgcac gacacggccc
     2401 agcttcgcac cgagctcctt cacgacctcc acctcgacga gcacggcttg gacctagcac
     2461 cgccggagtc gacgctcttc gcggcacccg atggcggcgg cgtcctgatc cagaccgccg
     2521 acccggttgc gactgcggcc gagatcgcgc gcgtctccgc gagcgacgcg cgg
//
LOCUS       DASPGK010000461         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_232937, whole genome shotgun sequence.
ACCESSION   DASPGK010000461 DASPGK010000000
VERSION     DASPGK010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232937"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VEC56_12950"
     CDS             <1..223
                     /locus_tag="VEC56_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15108.1"
                     /translation="ENKPDSLLTLDERRQKDVDAAHARWLARVEAGQKRAEELNARFA
                     KWYYVISSDSFDSLRLRRTDLLRDKPKQG"
     gene            complement(328..633)
                     /locus_tag="VEC56_12955"
     CDS             complement(328..633)
                     /locus_tag="VEC56_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV15109.1"
                     /translation="MTYVVTGNCQKCRFTDCVVVCPVDCFHADDEMLYIDPDSCIDCD
                     ACAPECPVEAIFPEEDVPDDQKQWTQINAERAAKLPLITDKQPELPTAQARKKELGF"
     gene            complement(685..1158)
                     /locus_tag="VEC56_12960"
     CDS             complement(685..1158)
                     /locus_tag="VEC56_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15110.1"
                     /translation="MERMRRGRFVALALAAAGAGLLAWAAVVWSRGSDSVETWRVRQA
                     EKESELRSTWDELKEFNLRYQAFQKSFPAVPDSIRMASGKQFREEGRTYEKAIRKLEF
                     AERDLKLEITNCKRKQAKAAAERKDRALPIAAGGAGAWLGAAVAAIASRPRREAA"
     gene            complement(1175..2299)
                     /locus_tag="VEC56_12965"
     CDS             complement(1175..2299)
                     /locus_tag="VEC56_12965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV15111.1"
                     /translation="MTPLDRLRPPRLLLLGDARQVHLRRWAAYFAEHGYDVLAVSLEP
                     GEGFPTAFRRIRVTAALPDAVRYPLAASVVRGIAGSFRPDVVNAHFVPNYGMIAALVF
                     GRRWVMSTWGSDVMTDPAKSAFHRWRVGFVLRRAGWVTSDAAVMTERIRDYGVPADRV
                     LTFPYGVDVSAFHPERPAASNGPRIVTNRKLEDVYSVDTVIDAFAAVREALPDATLTV
                     AGDGSLGASLRRRAERSPAAAAVTFVGAVDHARMPALLQSHHVYVSASRSDTTSVSLL
                     EAMACGLFPVVTDIEANREWIVDGESGRLVPPGEATRLAVAIIDAWRDSELRERALRI
                     NLRAVEQRARWEETMRPVKALFDEMATGASEDAPAAVRAD"
     gene            2470..3168
                     /locus_tag="VEC56_12970"
     CDS             2470..3168
                     /locus_tag="VEC56_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15112.1"
                     /translation="MRRVCLVVFLLATLASCRSKQHPVEVYVGPAMESDAQPRVEKIA
                     VLATATSLHESEDPDKLAPRTMERLLIQELDQRKDYKFIAPNLVNYVIEQKQWEDRYQ
                     KFLRSYAATDKIDMAFLGELSNELGCDAVLIQVVDLWQKDEVDITENATPATYVGATV
                     TIVGAKDGAILFRATDEDYLEGARTETADRSLVTSGSGAVYSDLGEKVHRAPPYDDVA
                     LKVAKALASSLPVR"
     gene            3213..3662
                     /locus_tag="VEC56_12975"
     CDS             3213..3662
                     /locus_tag="VEC56_12975"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXV15113.1"
                     /translation="MNDDARWMGEALAEAERAFAEGEVPVGAVVVLENRAIGRGRNRT
                     VTSGQPFEHAEVVALWDAVAGVGAHALEQAVLYSTIEPCVMCIGAVLLAKIPRVVFGA
                     REPRTGACESILAIPNEPRLLHRVVVVGGVEEKRCRSLMQRFFEARR"
     gene            3714..3803
                     /locus_tag="VEC56_12980"
     tRNA            3714..3803
                     /locus_tag="VEC56_12980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3748..3750,aa:Ser,seq:gga)
     gene            3856..4923
                     /locus_tag="VEC56_12985"
     CDS             3856..4923
                     /locus_tag="VEC56_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15114.1"
                     /translation="MGAFPSPASENRSAAWFCGDGAVTGVGSLPFVDAARAIDFVARH
                     APEVPFWPQLRRLSPREAAIPQVLGPAFKHVVVVRGEYAFHVPADRMDRFAAALARDD
                     AKLEPGNAAGFYAFLDAFARGAFPEARAAKGQIMGPVTTASALESGGHSLLQDVEMRR
                     VIAEHVARMANSQAEALLRVAPSAILVLDEAYLGVALRARPELRPAMVELLRLVVLRV
                     RRPGLLVGLHCCDELPFSVLNEIAPDVFSFDAHHGGAVFAADPDARRFVAEGGHVAWG
                     WVPTRDDLRGVDAAGVADRWWSVAQRMVEAGDDIDIARIRCRSLVTASCGLAGSSEAT
                     CEHSFTLAREVARRFVERADT"
     gene            4933..5133
                     /locus_tag="VEC56_12990"
     CDS             4933..5133
                     /locus_tag="VEC56_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXV15115.1"
                     /translation="MQRETSQHPSSPCTSLCVLDSATGRCQGCGRTLEEIGRWSAMTA
                     AEKWAVLRAVRARRGEQEPLDG"
     gene            5126..5524
                     /locus_tag="VEC56_12995"
     CDS             5126..5524
                     /locus_tag="VEC56_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXV15116.1"
                     /translation="MADIDAALKARIDASETRIAKVVAPATTNLYDTLFGGTLLSWMD
                     EVAFITATRFGRCPFVTVSIERIDFKHPIPAGTIVELIARVESVGRTSLKVRIDVFVE
                     HMLKADRLKAVTGSVTMVAIDEHKRPTPIP"
BASE COUNT          876 a         1838 c         1981 g          897 t
ORIGIN      
        1 cgagaacaag cccgacagcc tgctcacgct cgacgagcgc aggcagaagg acgtcgacgc
       61 cgcccacgca cgctggttgg cgcgcgtcga ggcggggcag aagcgcgcgg aggagctcaa
      121 cgcgcgcttc gcgaagtggt actacgtgat ctcgtcggac agcttcgaca gtttgcgcct
      181 gcgccgcacg gatctcctgc gcgacaaacc gaagcaggga tagcacgccc gcggtaggcg
      241 aggcccctgc tcccctgcat tccgaaagca caaaaggctc cggtcagccg accggagcct
      301 tctttcgaga ttcgcagacg cgccgcgcta gaacccgagt tccttcttgc gcgcctgggc
      361 ggtcggaagc tcgggttgct tgtcggtgat caagggaagc ttggcggcgc gttccgcgtt
      421 gatctgggtc cactgcttct ggtcgtcggg aacgtcctct tcggggaaaa tcgcctccac
      481 cgggcactct ggcgcgcacg cgtcgcagtc gatgcacgaa tccgggtcga tgtagagcat
      541 ctcgtcgtcg gcgtggaagc agtcgaccgg gcacaccacg acgcaatcgg tgaagcgaca
      601 cttctggcag ttgccggtta cgacgtacgt cattcacaac tccttcgaga ggtccgctag
      661 gatagtgcgc gcgaaggacc ggcgtcaagc ggcttcgcgc cgggggcgcg acgcgatcgc
      721 cgccaccgcg gcgccgagcc aagcgccggc gccaccggcg gcgatgggaa gcgcgcggtc
      781 cttgcgctcg gccgcggctt tggcctgctt gcgcttgcag ttggtgatct cgagcttgag
      841 gtcccgctcc gcgaactcga gcttgcggat cgccttctcg tacgtccggc cctcctcgcg
      901 gaactgtttt ccgctggcca tgcgaatcga atctggaacc gccgggaagc ttttctggaa
      961 ggcttggtag cgaaggttga actccttgag ctcgtcccag gtggaccgaa gctcggattc
     1021 cttttcggcc tggcgcaccc gccaggtttc gaccgaatcg ctcccacggc tccacaccac
     1081 cgcggcccac gccagcagac cggcgccggc cgcggccagc gccaatgcga cgaagcgccc
     1141 ccgtctcata cgttccatgt cgttgagttc ctttctagtc ggcgcgcacg gccgcgggcg
     1201 cgtcttcgct cgcgccggtc gccatctcgt cgaagagcgc cttgaccggc cgcatggtct
     1261 cttcccagcg ggcgcgttgc tcgacggcgc gcaggttgat acgcaacgcc cgctcgcgca
     1321 gttccgagtc gcgccacgcg tcgatgatgg caaccgcgag ccgcgtggcc tctccgggcg
     1381 gtaccagacg cccactctcc ccgtcgacga tccattcccg gttggcctcg atgtcggtga
     1441 cgacggggaa cagcccgcac gccatcgcct cgagcaacga gaccgacgtg gtgtcggagc
     1501 gcgaggcgga cacgtacacg tggtgcgact gcagcagcgc cggcatgcgc gcgtggtcga
     1561 ccgcacccac gaaggtgacg gcggcggccg cgggcgatcg ttccgcccgc cggcgaaggg
     1621 atgcacccag cgacccgtcg cccgcgaccg tcagggtcgc gtcgggcagg gcctcgcgca
     1681 ccgcggcgaa ggcgtcgatc acggtatcga cgctgtacac gtcctcgagc ttgcggttgg
     1741 tgacgatacg gggcccgttg gacgccgccg ggcgctcggg gtgaaacgcc gacacgtcga
     1801 cgccgtaggg aaacgtgaga acgcggtcgg ccgggacccc atagtcgcgg atgcgctcgg
     1861 tcatgacggc ggcgtcggag gtcacccagc cggcacggcg cagcacgaag cccacgcgcc
     1921 agcggtggaa cgcactcttt gcggggtcgg tcatgacgtc gctgccccac gtgctcatga
     1981 cccagcgccg gccgaagacg agggccgcga tcattccgta gttcgggacg aagtgcgcgt
     2041 tcacgacgtc ggggcgaaac gagcccgcga taccacgcac gacgctcgcc gcgagcgggt
     2101 agcgcacggc gtcgggcagc gctgcggtca cgcggatgcg acgaaacgcg gtggggaatc
     2161 cctcccccgg ctccagggaa accgccagga cgtcgtagcc gtgctctgcg aagtaggcgg
     2221 cccagcgccg caggtgcacc tggcgcgcgt ctcccaggag cagcagtcgc ggcgggcgga
     2281 ggcgatcgag tggggtcacg cggccagtgt agtgccgcgc cgccgcggtg tcaaagtcgg
     2341 ggggcgcgcg ctcactcggg gccgccgcgc ccggttgaca cccgttcgag cacgggctac
     2401 gctcacggtg acgggaagcg cgtcggaacc gaatcgattc aacggacgca cgaaggagtg
     2461 ctgaatccca tgagaagagt ctgccttgtc gtcttcttgc tcgcgacact cgcgtcgtgt
     2521 cgcagcaagc aacacccggt cgaggtctac gtgggtccgg ccatggagtc cgatgcccag
     2581 ccccgcgtcg agaagatcgc cgtgctggcg acggcgacgt cgctacacga gtcggaggac
     2641 cccgacaagc tggcgcccag gaccatggag cgcctgctca tccaagagct ggaccaacgc
     2701 aaggattaca agttcatcgc gcccaacctg gtcaactacg tcatcgaaca gaagcagtgg
     2761 gaagatcgct accagaaatt cctgcgctcg tacgcggcca ccgacaagat cgacatggcg
     2821 ttcctgggcg agttgtcgaa cgaactcggg tgcgacgcgg tgctgatcca ggtcgtcgac
     2881 ctgtggcaga aggacgaggt cgacatcacc gagaacgcga caccggccac ctacgtgggc
     2941 gcgacggtga cgatcgtggg cgccaaggac ggagcgattc tgtttcgggc gaccgacgag
     3001 gactacctcg agggtgcgcg cacggagacc gcggaccgct cgttggtgac gagcggatcg
     3061 ggtgccgtgt actccgattt gggtgaaaag gtccaccgcg cgccgcccta cgacgacgtc
     3121 gcgttgaagg tcgccaaggc gctcgcgtcc agcttgccgg tgcgctaggt ccgggccgct
     3181 tgggcccgcg gctgccggca cacttcgatc gcatgaacga cgacgcgcgt tggatgggcg
     3241 aggccctcgc ggaagccgag cgcgccttcg ccgagggcga agttccggtg ggtgcggtgg
     3301 tggtgctgga gaatcgcgcc atcgggcgcg gccggaaccg gacggtcacg agcggacagc
     3361 ccttcgaaca cgccgaagtg gtggcgctgt gggacgcggt ggccggcgtg ggggcgcacg
     3421 cgctcgagca ggccgtgctg tactcgacca tcgagccgtg cgtcatgtgc atcggcgcgg
     3481 tgttgctggc aaaaattccg cgcgtcgtgt ttggcgcgcg cgagccgcgg accggggcgt
     3541 gcgagagcat cctcgcgatc cccaacgagc cgcggctcct gcatcgcgtg gtggtcgtgg
     3601 gtggcgtgga ggaaaagcgg tgccggtcgc tgatgcagcg attcttcgaa gcccggcgtt
     3661 aggcccccgg gacacgatcg cgagatttcc ggacacgacg gattcgattc ggcggagaga
     3721 tgcgagagtg gctgaatcgg cacgattgga aatcgtgtga gcgcgaaagc gctccgaggg
     3781 ttcgaatccc tctctctccg ccattcactt tttgcgcgcg ccccgtgcag gccggcgcgc
     3841 gctccagaaa tgaccatggg cgcttttcca tccccggctt cggagaatcg ctccgccgcg
     3901 tggttttgcg gcgacggcgc ggtcacgggt gtcggtagcc ttcccttcgt cgacgcggcg
     3961 cgcgcgatcg acttcgtggc gcgccacgcg cccgaggtcc ccttctggcc gcagctgcgg
     4021 cggttgtccc cgcgcgaagc cgcgattccg caagtgctgg ggcccgcgtt caagcacgtg
     4081 gtggtggtgc ggggcgagta cgcgttccac gtcccggccg atcgcatgga ccgcttcgcg
     4141 gccgcgcttg cgcgcgacga cgccaagctg gagcccggca acgcggctgg tttctatgcc
     4201 tttctggacg cgttcgcccg cggcgccttt cccgaggccc gcgccgccaa ggggcagatc
     4261 atggggccgg tgacgacggc ctccgcactc gaatcgggcg gacattcgct cttgcaggac
     4321 gtcgagatgc ggcgcgtgat cgcggaacac gtggcgcgca tggcgaactc gcaggccgag
     4381 gcgctcctgc gcgtcgcgcc gtcggcgata ctcgttctcg acgaagccta cctcggcgtg
     4441 gcgctgcgtg cgcggccgga gctgcgcccg gccatggtcg agctcttgcg cctggtcgtg
     4501 ctgcgcgtgc gccggcccgg gctgctggtc ggtctgcatt gctgcgacga gctgccgttc
     4561 tcggtcttga acgaaatcgc ccccgacgtc ttctcgttcg acgcgcacca cggcggcgcc
     4621 gtcttcgcgg ccgatccgga cgcgcgccgc ttcgtggccg agggcggcca cgtggcgtgg
     4681 ggctgggtgc cgacgcgcga cgacctgcgc ggcgtcgacg cggccggcgt ggcggatcgc
     4741 tggtggagcg tcgcgcagcg catggtcgaa gcgggcgacg atatcgacat cgcgcgcatc
     4801 cgctgtcggt cgctcgtcac cgcgtcgtgc gggctggccg gctccagcga ggcgacgtgc
     4861 gagcattcgt tcacgctggc gcgcgaagtc gcgcgccgct tcgtcgagcg ggcggataca
     4921 tagtagggcg acatgcagcg cgaaacctcc cagcatcctt cgtcgccgtg cacgagcctg
     4981 tgcgtgctcg attccgccac cggccggtgc cagggatgcg ggcgcacgct ggaagagatc
     5041 gggcgctggt cggcgatgac ggcggcggaa aagtgggccg tgctgcgggc ggtccgggcg
     5101 cgtcggggcg agcaggagcc actcgatggc tgacatcgac gccgccctca aagcgcgcat
     5161 cgacgcgtcg gagacgcgga tcgcgaaagt ggtggcgccg gccaccacca acctctacga
     5221 cacgctcttc ggcggcaccc tcttgagctg gatggacgag gtggcgttca tcaccgcgac
     5281 ccgctttggg cggtgtccgt tcgtcacggt gtcgatcgag aggatcgact tcaagcaccc
     5341 gatcccggcc gggaccatcg tcgaattgat cgcgcgggtg gagtccgtgg gccgaacgag
     5401 cctcaaggtg cgcatcgacg tgttcgtcga gcatatgttg aaggccgacc gcctgaaggc
     5461 cgtgaccggt tcggtcacga tggtggcaat cgacgagcac aagcgtccga cgccgattcc
     5521 ctaggcttgc ctttccgggc gatggtgggt tagtattcgc ccggagagat gccagagtgg
     5581 ttgaatgggg cg
//
LOCUS       DASPGK010000462         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1052631, whole genome shotgun sequence.
ACCESSION   DASPGK010000462 DASPGK010000000
VERSION     DASPGK010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052631"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VEC56_13000"
     CDS             <1..349
                     /locus_tag="VEC56_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXV15117.1"
                     /translation="FEIVPMGEGFTWETKNRDRDTAVAWKTWEACRGAIEENRHDLLI
                     FDEINNAIHYGYLDVGVVIDALKKKPPEMHIILTGRDAHPDLVDVADLVTEMREIKHP
                     FHRGIYAQRGIEY"
     gene            complement(437..616)
                     /locus_tag="VEC56_13005"
     CDS             complement(437..616)
                     /locus_tag="VEC56_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HXV15118.1"
                     /translation="MGVWKDMKSRFSIFGELWAFMRVRKKWWLGPIMIFLVLLGVLIV
                     FTEGSALAPFIYALF"
     gene            768..1562
                     /locus_tag="VEC56_13010"
     CDS             768..1562
                     /locus_tag="VEC56_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV15119.1"
                     /translation="MDFGIRGRAAAVAASSQGLGFACALELAREGAAVAICSRDRGRV
                     DDAARRIREAVRDAKVHAVVADLAKDADCRTFVDEAARTFGRLDILVTNSGGPTPGSF
                     DQITLDDVRRGVDSTLMSAVALMSAAVPHLRKNAWGRIVNILSVTVKQPKTTLLVSNT
                     MRPGILGFAKSISFELAQDNITVNNVAPGYTKTERLDELAEHLSSTQKRSQEDVFAEW
                     ERTIPAGRLGRPEELAAMVAFLCSERASYVTGVTIQVDGGQVQSLL"
     gene            complement(1566..2480)
                     /locus_tag="VEC56_13015"
     CDS             complement(1566..2480)
                     /locus_tag="VEC56_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV15120.1"
                     /translation="MTPTTAKRTRPQPADARREQVEAKIHQSAYANWFLIATVVLLTL
                     VSLVSVLPPLFSEQIASFWLFSKPQMMVITGLTLTLLLLVGLAHQRRYLVALRRQFEA
                     IQRAERERMQRQAARTYALLNVSRVMVEQNDIQTVFDSITKICVDAFSCDQASLMLFD
                     RESTTLVVRAASGKTIPEGMLGTRQPLGKGVAGFVAQQREPLVLRADQRTSIPGLELK
                     NREITAAMIVPVILREELVGVINVSTRSPEVNFDDDDLRALQVFAENVGAAIRHTEQA
                     EWMRATIRKLQSQKDRSNITGVYPAQNP"
     gene            complement(2661..>3224)
                     /locus_tag="VEC56_13020"
     CDS             complement(2661..>3224)
                     /locus_tag="VEC56_13020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV15121.1"
                     /translation="VYDLALPVLARYGFRASLFVVTGRVGTQWDEVNTLDWSRLRELD
                     QSGVFRIESHSHDMHYKVSAEGTARPVFVAASAHGYQVERTANWEDAVRDDLMRSRHE
                     IERHIGRESAFLAWPYGEATPELDRIAREAGFLRTCTLRARPVARMAPAGGDSTPPIE
                     ISRYTITARTSLRDFRAMLDGTYRPNV"
BASE COUNT          562 a         1021 c         1103 g          538 t
ORIGIN      
        1 cttcgagatc gtccccatgg gcgagggctt cacctgggag accaagaacc gcgaccgcga
       61 caccgcggtg gcgtggaaga cctgggaagc gtgccgcggc gccatcgaag agaaccggca
      121 cgatctcctg atcttcgacg agatcaacaa tgccatccac tacggctatc tcgacgtggg
      181 cgtcgtcatc gatgccctca agaagaagcc gcccgagatg cacatcattc ttaccggacg
      241 cgacgcgcac ccggacctcg tcgacgtcgc cgacctggtg accgagatgc gcgagatcaa
      301 acacccgttc caccgcggca tctatgcgca gcgggggatc gagtattgac gagcgtcccc
      361 cggcgggtgg cgctcgtggg gcccccgcgc cgtcagggcg cggggtgacg ggtgacagga
      421 cccgccgggc agcgttctag aacagcgcgt agatgaacgg cgccagcgcg gatccttcgg
      481 tgaagacgat cagcacgccg agcaggacca ggaaaatcat gatggggccg agccaccact
      541 tcttgcgaac gcgcatgaat gcccacagtt cgccgaagat cgaaaaacgg ctcttcatgt
      601 ccttccaaac acccatggaa tgcagctcct gaagcgggtc tagggttatg cgcggtggcg
      661 cctgcggggc gaccgtgcgg cacaatcggt ggcgttcatt atatgggatt cggccgcgtc
      721 gtgtccaccg gggcgacgtc gaagcgcggg catcctagga ggagagaatg gacttcggaa
      781 ttcgaggccg cgcggccgcg gtggccgcgt cgagccaggg attgggattc gcgtgcgcgc
      841 tcgaactcgc gcgcgaaggc gcggcggtgg cgatctgctc gcgcgaccgc gggcgggtcg
      901 acgacgcggc gcggcgcatt cgcgaagccg tgcgcgacgc gaaggtgcac gcggtggtcg
      961 cggacctcgc gaaggacgcg gattgccgga cgttcgtgga cgaagccgcg cgcacgttcg
     1021 ggcgtctcga tattctcgtg accaactcgg gcggacccac gcccggatcc ttcgaccaga
     1081 ttacactcga cgacgttcgt cgcggcgtcg attccacttt gatgagcgcg gtcgcgctca
     1141 tgtccgcggc cgttccgcac ctgcgcaaga acgcctgggg gcgcatcgtc aacattctgt
     1201 cggtcacggt gaaacagccc aagacgacgc tcctggtgtc gaacacgatg cggcccggta
     1261 tcctcggctt cgcgaagtcg atctcgttcg agctcgccca ggacaacatc acggtcaaca
     1321 acgtggcacc cggctacacg aagaccgaac gcctcgacga actggccgag catctttcgt
     1381 cgacgcagaa gcggtcgcag gaggacgtat tcgcggagtg ggaacgaacc atccccgccg
     1441 gccgcttggg acgcccggaa gaattggccg cgatggtcgc gttcctgtgc tcggagcgcg
     1501 cgagctacgt caccggcgtg acgatccagg tcgacggagg ccaggtgcag agcttgctgt
     1561 agctgctaag ggttctgcgc ggggtagacg cccgtgatgt tgctgcggtc tttttggctt
     1621 tggagcttgc ggatggtggc gcgcatccac tcggcctgct cggtgtgacg gatcgcggca
     1681 ccgacgttct cggcgaacac ctgcagcgct cgcagatcgt cgtcgtcgaa gttgacctcc
     1741 ggcgagcgcg tgctgacgtt gatcacgccc accagctctt cgcgcaggat cacggggacg
     1801 atcatggccg ccgtgatctc ccggttcttg agctcgaggc ccggaatgct ggtacgctga
     1861 tcggcgcgca gcaccagcgg ctcgcgctgt tgcgcgacga atcccgccac gcccttgccc
     1921 agcggctgcc tcgtccccag cattccctcg gggatcgtct tgccgctggc cgcgcgcacg
     1981 accagcgtcg tgctctcgcg atcgaacagc atcagcgagg cctggtcgca cgagaaggcg
     2041 tccacgcaga tcttggtgat cgaatcgaac acggtctgga tgtcgttctg ctcgaccatc
     2101 acgcggctga cgttcaagag ggcgtaggtg cgcgcggctt ggcgctgcat gcgctcgcgc
     2161 tcggcacgct ggatcgcttc gaactgacgg cgcagcgcca cgaggtagcg gcgctggtgc
     2221 gcgaggccga ccaggaggag caaggtcagc gtgaggcccg tgatcaccat catctgcggc
     2281 ttcgagaaca accagaacga cgcgatctgc tcgctgaaaa ggggcggcag aacggacacg
     2341 aggcttacca gcgtcaacag cacgacggtc gcgatcaaga accagttcgc gtaggcgctc
     2401 tggtggatct tggcttcgac ctgctcgcgc cgcgcgtcgg ccggctgcgg tctggttctc
     2461 ttcgcagtgg ttggcgtcat ttttcggggt tcccggggcc cttcctgccc gactcgatac
     2521 agaattcgac ggcttagggg caaggagcgt gccaccggcg gaaaccggcg gcacgacgcg
     2581 gaattccagg ggtggatggg ccgcgctgcg ttgcagatcg acgccgccgg ttgcggaccg
     2641 cccgggggtc tagagaagac ctagacgttg gggcggtagg taccgtcgag catggcgcgg
     2701 aagtcgcgca gcgaggtacg cgcggtgatg gtgtagcgcg agatctcgat gggtggcgtg
     2761 gaatcgccgc cggccggcgc cattcgcgcc acggggcgcg cgcgcagcgt gcacgtgcgc
     2821 aggaatcccg cctcgcgcgc gattcgatcg agctcgggag tagcctctcc atagggccag
     2881 gcgaggaagg cggactcacg gccgatgtgc cgctcgatct cgtggcgcga gcgcatgagg
     2941 tcgtcgcgga cggcgtcttc ccagttcgcc gtccgctcga cctggtagcc atgcgcgctc
     3001 gcggccacga acacggggcg cgcggttcct tccgcgctca ccttgtagtg catgtcgtgc
     3061 gagtgcgact cgattcgaaa cacgccgctt tgatcgagct cgcgcagccg gctccaatcg
     3121 agcgtgttga cttcgtccca ctgcgtcccc acgcgtccgg tgacgacgaa cagcgacgcg
     3181 cgaaatccgt agcgcgcgag aacaggaagc gccagatcgt atac
//
LOCUS       DASPGK010000463         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Krumholzibacteriia bacterium isolate
            SMAG_U16449 k141_1403908, whole genome shotgun sequence.
ACCESSION   DASPGK010000463 DASPGK010000000
VERSION     DASPGK010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935444
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Krumholzibacteriia bacterium (soil metagenome)
  ORGANISM  Candidatus Krumholzibacteriia bacterium
            Bacteria; Candidatus Krumholzibacteriota; Candidatus
            Krumholzibacteriia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 18:37:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,607
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Krumholzibacteriia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403908"
                     /isolate="SMAG_U16449"
                     /isolation_source="soil"
                     /db_xref="taxon:2774603"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEC56_13025"
     CDS             complement(<1..706)
                     /locus_tag="VEC56_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV15122.1"
                     /translation="MTATTPPDVFTHPRLEYEALTHTVGLIDLAHVGVLRLRGGDRVR
                     FLNAMVTNDVAALHSGSACEALLTTTKGRVVAELLILARADELLVLVMQGSTQRVFEA
                     LDSHIIADDVQLTDMSAEMSVLSLEGPKSREVVWRIFPRAPLPLESLKFTENEYQGLH
                     AMVVRHSVAGEKGMHVIVSRGQAERLRDYLVQAGVGMDMQVVGRVAWNTRRVEAGLPW
                     YGIDVSEDNFPKEARLD"
     gene            complement(708..1088)
                     /locus_tag="VEC56_13030"
     CDS             complement(708..1088)
                     /locus_tag="VEC56_13030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXV15123.1"
                     /translation="MRTDYGLIALKHIAALPAGDLANAREIAARFNLPPNLLAKILQN
                     LSQSGIIEATKGSGGGYRMVKSPHDVTLAQIFESIEGPVHMVMCTSNDGCCSIEDRCT
                     VKNGLTNLEQRFYQFFDTVTLADV"
     gene            complement(1164..2291)
                     /locus_tag="VEC56_13035"
     CDS             complement(1164..2291)
                     /locus_tag="VEC56_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXV15124.1"
                     /translation="MSTQSSKHSPWLLDEVRRFDPSVPLEAAWLPPKSWYADSRFFDL
                     ECETVFKNNWLIAARSDQFKKAGDFVAGSVAGEPYVVVRGEDGALRAFFNVCRHHAAV
                     VAVGAGCSESLVCPYHGWTYGLDGRLLRAPELGAVKDFDREKFGLVPMKVGEWCGLVF
                     VSMSKEPRPLSNDLTEVARRLQEMNVHQLQFVARRKYTLACNWKVYVDNYLDGGYHVS
                     YLHKGLAGQLDLDSYKTEIFERYSIQSGAGAGDAGEKGGGDFSERIGDKVLYAWIYPN
                     LMINRYGPMMDTNWVIPKGNAETDVIFDYYFAPETARDTDFVEKSLAASDVVQQEDVE
                     ICESVQRGLGSSSYDQGRYSVKREHGEHHFHKLLSADFRAV"
BASE COUNT          416 a          798 c          739 g          432 t
ORIGIN      
        1 tatcgaggcg cgcttccttg gggaagttgt cttcgctgac atcgatcccg taccagggca
       61 gcccggcttc gacccgtcgc gtgttccacg cgacgcgccc caccacctgc atgtccatcc
      121 ccacgccagc ttgcaccaga taatcgcgca ggcgctcggc ttgcccgcgc gacacgatca
      181 cgtgcatgcc tttctcgccc gccaccgagt ggcggacgac catcgcgtgc aggccctggt
      241 actcgttctc ggtgaacttg aggctctcca aagggagcgg cgcgcgcggg aagatgcgcc
      301 acacgacttc gcgcgacttg ggtccttcca atgaaagaac cgacatctcg gcgctcatgt
      361 cggtgagctg cacgtcgtcg gcgatgatgt gcgagtcgag ggcctcgaat acgcgctgcg
      421 tggagccttg catcacgagt actagcagct cgtcggcgcg cgcgaggatc aacagctccg
      481 cgacgacgcg tcccttggtg gtcgtgagca gcgcctcgca cgccgagccg gaatgtagcg
      541 cggccacgtc gttggtcacc atcgcgttca ggaagcgaac ccggtcgccg ccgcgcagac
      601 gcagcactcc gacgtgcgcc aaatcgatca agccgacggt gtgggtcagc gcttcgtact
      661 cgaggcgcgg gtgggtgaag acgtccggcg gggtggtcgc ggtcatgcta gacatccgcg
      721 agcgtcaccg tgtcgaagaa ctgatagaag cgctgctcga ggttggtgag gccgttcttc
      781 accgtgcacc gatcctcgat cgagcagcac ccgtcgttgc tcgtgcacat gaccatgtgc
      841 accggtccct cgatcgattc gaagatctgc gccagcgtga cgtcgtgcgg gctcttgacc
      901 atgcggtagc cgccgccgct gcccttggtc gcctcgatga tcccgctctg cgagaggttc
      961 tgcaggatct tcgcgagcag gttgggcggc aggttgaagc gcgccgcgat ctcgcgcgcg
     1021 ttggccaagt cgcccgcggg cagggccgcg atgtgcttga gcgcgatcag cccgtaatcg
     1081 gttctcatgg agatgcggat catcgcccat ataataatga gcggggcgcg cggattcaat
     1141 gaccggaatc gggcgttggg gggctacacg gcgcgaaagt ccgccgacag gagcttgtgg
     1201 aagtggtgct ctccgtgctc gcgcttgacc gaatagcgcc cctggtcgta cgacgacgac
     1261 cccaggcctc gttgtaccga ctcgcaaatc tccacgtctt cctgctggac cacgtcgctg
     1321 gcggccaggc tcttctccac gaagtccgtg tcgcgcgcgg tctcgggggc gaagtagtag
     1381 tcgaagatga cgtcggtctc cgcattgccc ttcgggatca cccagttggt gtccatcatg
     1441 gggccgtagc gattgatcat gagattggga taaatccacg cgtagaggac cttgtcgccg
     1501 atgcgctcgg aaaaatcgcc gccgcccttc tcgcccgcat cccccgcgcc cgcgccggat
     1561 tggatcgagt agcgctcgaa gatctcggtc ttataggaat ccagatcgag ctgccccgcg
     1621 agacccttgt ggagataact cacgtggtag cccccgtcga gatagttgtc gacgtagacc
     1681 ttccagttgc acgccagcgt gtacttgcgc cgcgcgacga actgcagttg gtggacattc
     1741 atctcctgca agcgccgcgc gacttcggtg agatcgttcg agagcggccg cggctccttc
     1801 gacatggaga cgaacaccag cccgcaccat tcgcccacct tcatcggtac caagccgaac
     1861 ttctcgcggt cgaagtcctt gaccgcaccc agctccggcg cccgcagcaa gcgcccgtcg
     1921 agtccgtacg tccacccgtg atacgggcac accagcgact ccgagcaccc tgcccccacc
     1981 gcgaccaccg ccgcgtggtg ccggcacacg ttgaaaaacg cgcgcagcgc tccgtcctcg
     2041 ccccgcacca caacgtacgg ctcgcccgcc accgaccccg ccacgaagtc gcccgccttc
     2101 ttgaactggt cgcttcgcgc cgcgatcaac cagttgttct tgaagaccgt ctcgcactcc
     2161 aggtcgaaga agcgcgagtc cgcgtaccac gacttgggcg gcagccacgc cgcctcgagc
     2221 ggcacactag ggtcgaagcg acggacttcg tcgaggagcc agggggagtg cttgctggac
     2281 tgagtcgaca tcgcggggaa gtgtatcacg cccgcgaatg tggtcgctac gtctcccccg
     2341 actcttgcac gggcttgtcg ggccacgcga tcgcgaatac aatgg
//
